LOCUS       DAENCV010000001       103546 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAENCV010000001 DAENCV010000000
VERSION     DAENCV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 103546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.001"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            4..918
                     /gene="murB"
                     /locus_tag="KQD82_000001"
     CDS             4..918
                     /gene="murB"
                     /locus_tag="KQD82_000001"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG4378512.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            922..1635
                     /locus_tag="KQD82_000002"
     CDS             922..1635
                     /locus_tag="KQD82_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG4378513.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1666..2523
                     /gene="rapZ"
                     /locus_tag="KQD82_000003"
     CDS             1666..2523
                     /gene="rapZ"
                     /locus_tag="KQD82_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG4378514.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2535..3641
                     /locus_tag="KQD82_000004"
     CDS             2535..3641
                     /locus_tag="KQD82_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG4378515.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3710..4135
                     /locus_tag="KQD82_000005"
     CDS             3710..4135
                     /locus_tag="KQD82_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG4378516.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4152..5099
                     /gene="whiA"
                     /locus_tag="KQD82_000006"
     CDS             4152..5099
                     /gene="whiA"
                     /locus_tag="KQD82_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG4378517.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5256..8825
                     /locus_tag="KQD82_000007"
     CDS             5256..8825
                     /locus_tag="KQD82_000007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG4378518.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9034..9993
                     /gene="pfkA"
                     /locus_tag="KQD82_000008"
     CDS             9034..9993
                     /gene="pfkA"
                     /locus_tag="KQD82_000008"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG4378519.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10045..11805
                     /gene="pyk"
                     /locus_tag="KQD82_000009"
     CDS             10045..11805
                     /gene="pyk"
                     /locus_tag="KQD82_000009"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG4378520.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            11980..13614
                     /gene="rlmD"
                     /locus_tag="KQD82_000010"
     CDS             11980..13614
                     /gene="rlmD"
                     /locus_tag="KQD82_000010"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG4378521.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSENLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDEFDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     MREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            13633..13914
                     /locus_tag="KQD82_000011"
     CDS             13633..13914
                     /locus_tag="KQD82_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378522.1"
                     /translation="MRWILKIILFPISLLLSILTAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALIIGFLVSPYGMPMVGATVIAVLQGVNERIRLV"
     gene            14055..14978
                     /locus_tag="KQD82_000012"
     CDS             14055..14978
                     /locus_tag="KQD82_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378523.1"
                     /translation="MSSYNFNYIIPDNFNRRTFQYLQQKNSNLATTFARCTYEYEDVG
                     LAYYAGLKTGDNWNKHALDFTIEGNQNDIEILQQLNSDLKDAIDKALKPSVSGLIIRH
                     IYYLPSEESTLDLPQSNVARLNADISTAEGVLKDLIQVSERLCSNSLYSDISLENSIN
                     DYFRDALSFMGYNEVKDQTRHGVSISEKDAGEVDLLLTKSGKEIAIFEGLKLDSVNSK
                     YIDEHIKKAIDNYNALGTATFVVAYVNVVNYDRFWEKYYEHISNYAHNMECKSAMKEL
                     THPNAATRIAYEVLSKDGYDFPVYFMTIKIA"
     gene            15124..16140
                     /locus_tag="KQD82_000013"
     CDS             15124..16140
                     /locus_tag="KQD82_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4378524.1"
                     /translation="MSMYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGK
                     NYHDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWY
                     TINNDKLEELYVDMELKKQKIQTEALKKSMPNTLGQNAPMDKGKINQCNNADCTNAQV
                     QNALMQRDNLHQPLPENTTNNTTNNSADISIHPSIKEKNQLFSENMKGRRIDISYKNI
                     YQNCLEELRHQTGYYEHMSIGNKIVAEEFDEILKILADVMVLDDESFITINQTKISVQ
                     VVKERFRELNSGHLEYLVGVLRENEYKIRNIRAFVLTAAYNAPTGIDAYYSALVSYDM
                     RGGY"
     gene            16142..16627
                     /locus_tag="KQD82_000014"
     CDS             16142..16627
                     /locus_tag="KQD82_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG4378525.1"
                     /translation="MINEEIARKTLNMEVRAAKVTGKLILDLLKKLMKEAEKLGGLEK
                     LVNVNGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKDKETGKYSVF
                     FQAKDMKVMDKAFKHALSESEKKAEMKESIHKNIEKFKEIAKNSISKDKIKNKQKEQS
                     L"
     gene            16624..18405
                     /locus_tag="KQD82_000015"
     CDS             16624..18405
                     /locus_tag="KQD82_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG4378526.1"
                     /translation="MIDKILKEIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNIMSFLPSLHPTDILIGIGVAALIKFIVYTKGKNAKKFRQG
                     KEYGSARWGTRKDIEPYMDEKFQNNILLTQTERLTMNGRPANPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKAGEDFWIKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDDETYMNPIDRLFEALEKREPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMKEDELELDTLGDRKAALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLSVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDK
                     RNLFDIESYIKRKGKVKMNRHTVITRL"
     gene            18569..19435
                     /locus_tag="KQD82_000016"
     CDS             18569..19435
                     /locus_tag="KQD82_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4378527.1"
                     /translation="MNIIKSFNSTIDYLETVLDDEIDEKKVTRISGYSYSMFSRLFSI
                     LTEMTLSEYLRSRRLTEAAIILRDTDEKIIDVAFKFGYESSDSFGTAFKNFHGFTPSE
                     VRNGKPFKLVSRVQLALSVRGGRSMNIRIQKKKAFTVAGVNQQNINSSICASLWQKLF
                     SKVSHEEMLNLGTGEAVGVCHDILSLDNHDVLDSNTINYMAGYIVTDVDKARSMGLDI
                     LEIGETEYAVVELTGSVPECIHNGWKYAMEVFFPEHGYVHSGKPDFEYYYEGDMYSKD
                     YKMELWIPVCKS"
     gene            19649..19960
                     /locus_tag="KQD82_000017"
     CDS             19649..19960
                     /locus_tag="KQD82_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG4378528.1"
                     /translation="MKQEMININANLLAEPTFSKFEKEGEKVEVANFTLVKKYGKGKE
                     YINCAAYGEKAEIAKAFEKGDLIHIFGYFKKREKEGKTYKNFVVKSYNKIEKKEKNEE
                     E"
     gene            19964..20179
                     /locus_tag="KQD82_000018"
     CDS             19964..20179
                     /locus_tag="KQD82_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG4378529.1"
                     /translation="MEFFGQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLR"
     gene            20199..21062
                     /locus_tag="KQD82_000019"
     CDS             20199..21062
                     /locus_tag="KQD82_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378530.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGQVFSFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSATVSGDQIVAMMDTLKEKG
                     LGELVMILFETSLIKVAIQVISVVIMLVVYGRMFEIYVYSSVSAIPFATMGNKEWGQI
                     GTNYIKGLFALGLQGLFLMVCLGIYAVLVKTIQITDIHTSTMTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            21082..21345
                     /locus_tag="KQD82_000020"
     CDS             21082..21345
                     /locus_tag="KQD82_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4378531.1"
                     /translation="MNKRKTVFTAVVIGAGIMAVIDRIRFHNKVDELEERTEDIGRCH
                     NDFCLMQQRYNRSTDEQIVSIQEEIGSVYEHIEELSKEKEDGR"
     gene            21349..21738
                     /locus_tag="KQD82_000021"
     CDS             21349..21738
                     /locus_tag="KQD82_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG4378532.1"
                     /translation="MAYVPIPKDLKKVKTKVAFNLTKRQLIGFTIAGLIGIPIYLFMR
                     KVVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     RQKKDSAAKVRTKQKEVKKSKTGLKAK"
     gene            21650..24079
                     /locus_tag="KQD82_000022"
     CDS             21650..24079
                     /locus_tag="KQD82_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4378533.1"
                     /translation="MKDKRKIQQLKLEQNKKKLRSQRQDLKQNKGKSKKDKGGLLDLI
                     FRKEPKRYTVEDTIPYLRLLKSGICQIDEKHFNKSIAFEDINYQLALEEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKGDGFDDIRLEFREMLKSQIAKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERMEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFEFSYKDLKKKESTKTYIAPDEFNFTPSRYFKFGKFIGAASHFQILASELS
                     DRMLAEFLDIDDNINISFHIKAIEQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVTIIFMNFARTIQKLDNTISQISSIANKH
                     NCKLKRLDHSQEQGLISVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSVNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILTTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFENPLPENMPILQNLYDMLKGQEEKVGKKLATEMEIY
                     VTGSLNVFNHQSNVDLNKQLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISQPQLKYVTNSNAGEGLLFFGNTIVPFLDKFPKDTILY
                     QKMTTKPEEVR"
     gene            24084..26183
                     /locus_tag="KQD82_000023"
     CDS             24084..26183
                     /locus_tag="KQD82_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG4378534.1"
                     /translation="MGKKLKRDFKERQRARIEKETLQSSGSIESEESKLKHNDDYRGK
                     IVHDKDRFQDKVHEKTSKIISDSEPVRGISKRNTRYRSSETGSVSREEIGKADYATEV
                     NDGIIDRYDNDFRGSEYFESTYDVEDNLHHKAEDTKGFLKNQKAQKKNYKRKNYSDKL
                     YTRKKDTEAKAEKADNKQGGKEAILDREKKNKLSKEQETIFKNKASKVSALSGLAKGS
                     ETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKNYSDKKRAQKGYDLINKDYKIRK
                     RKSKLEFRDVKEELKKTEEYQKANNFKRFQKRKQMKDSIKRQNKSRLRDRIKEGLIGS
                     LKGSKEIILRKTKGLMIIFIGLIILGTFFINFAGTGMTGFMNSTSIVLTTSYLSKPNV
                     LTEINQQFSSMEQGLQDELESVEENHPGYDEYIVNKEEDIGHNTHELLSYITSRCGEV
                     KNISEVNRILDELFKSMYRVDYKEEIEIRYKTVAETYTDEDGNEYTESHEEPYEYKKL
                     IVTLYKREMDSVVREVFQNYPDNVKHYEALLASLGNMSDFFGSGSNDYSEIINNPNFG
                     NPGIAFNEETVKRLFSEAERHIGKRYVFGANGPSNFDCSSFVCWSFTRSGIKNMPRTT
                     AWRIYTDYCNPVSTGEAKAGDIIFFKNTYNSGSPISHVGIYAGNGMMIHAGDPIQYTS
                     INSPYWREHFYGFGRPR"
     gene            26195..26428
                     /locus_tag="KQD82_000024"
     CDS             26195..26428
                     /locus_tag="KQD82_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4378535.1"
                     /translation="MKKELTAVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITIYDLMAKVNREKSEMKKENGNEKNEE"
     gene            26409..27977
                     /locus_tag="KQD82_000025"
     CDS             26409..27977
                     /locus_tag="KQD82_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG4378536.1"
                     /translation="MKKMKNKKAAGVILGAVLLIVGVVISLFAGSKIVFAQGGLFTTL
                     VNPIEAVAQIEVEVNVKYIFEDEKVFKEEKIKAEKGQLLDSGDLPIIPDDMKFIDDFL
                     FYEIKGDGKDEIIRKVAKVEVKDKETQTENESSKSEQSSQSNASKKENKGTQTELSKD
                     NISKMEKESKELQEKLDKLNQEIKDKDKLSDKQKDKIKDLEDKIDKLKGKLEKSKADK
                     DLSTDMKKEMDKLTDKIKELEKKANEVGRAPVTHNPTVAPINPISGIKQGTGNVSPQA
                     SINSSGKGSASQESKGANTKNIDSDKSKEKEKEVRYPNKLTPKQPANNGSQDSSLDGS
                     SKNVNTNKGIASAPSKARGAVTENKDNANKDYPIHHNDSKDNKEMDKYSADARQFVTF
                     TTKNGKTFHLIINHDENSENVMLLTEVSEDDLLNMVEKKAPEKEVVKEEPQKEELKPV
                     KKEESSNTGIYLILILVVAGALGAGYYFKVIKKKEDKELEGFEEEDDDFFSEAEESEN
                     ESDETEDKEDDEVE"
     gene            28074..29780
                     /locus_tag="KQD82_000026"
                     /pseudo
     CDS             28074..29780
                     /locus_tag="KQD82_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
     gene            29865..30050
                     /locus_tag="KQD82_000027"
     CDS             29865..30050
                     /locus_tag="KQD82_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG4378537.1"
                     /translation="MSEAFMNNAKVMSKGQVTIPKKIREILEIENGDYVTFVVTEGKI
                     QIVNSKTFIEKNIQGRK"
     gene            30084..31130
                     /locus_tag="KQD82_000028"
     CDS             30084..31130
                     /locus_tag="KQD82_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBG4378538.1"
                     /translation="MNKEKNSLQNINIEKNNNSFEEIVMIVENAKDRAYRRVNEELIL
                     MYQEVGKYISEKTKEASYGSGFVDNVAEFFSINYPDLKGFNRRGLYRMKQFYELYKDD
                     GKVSTLLTQLSWSNHLKIMSGAKSREEREFYINLAIKENLTHRELVRQMDSGYYERCM
                     LSNEGNMPAIQRAKQETHNLFMDSYVLEFLDAPKIGNERDFQKSILENLKNFVLEIGK
                     DFSFIGNEYRVQVGNHDYYIDLLFYHRGLSCLVAFELKIGEFKPEYVGKMNLYLEALD
                     REVKKQAENPSVGVILCASKDDEVVEFALSRSLSPTMVSEYRLKLIDKELLQRKLKEY
                     TGIAEQEIERYDEK"
     gene            31240..32046
                     /locus_tag="KQD82_000029"
                     /pseudo
     CDS             31240..32046
                     /locus_tag="KQD82_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            32160..41147
                     /locus_tag="KQD82_000030"
                     /pseudo
     CDS             32160..41147
                     /locus_tag="KQD82_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            41202..41861
                     /locus_tag="KQD82_000031"
     CDS             41202..41861
                     /locus_tag="KQD82_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG4378539.1"
                     /translation="MNYKEIRNTLEQMANESHEDFAKALISFEKGINDKEALDKLYQE
                     YMENDSMSLLNDEFDYLIEELRENGQIKDTAAIEKEDNDLVNIVGNIVGEVDVVEREN
                     KNGEVFKVVNFSVASKDDDGSKTYTNCSAYGDKVEIPKDFKQGDFVKLFGQLRTSIDD
                     NGKEYSNVRILSSKLLKAKEQMKNQEEKKESVLGAIKKYKAEEKAKPTEKKEALKETE
                     R"
     gene            41991..42209
                     /locus_tag="KQD82_000032"
     CDS             41991..42209
                     /locus_tag="KQD82_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378540.1"
                     /translation="MKFNYNGLWKVLIDKNMKKKDLIDRTGISPTTISKMVRGEAVSL
                     SVIGKICEELGTDIGDLIHIEKGIEEDK"
     gene            42210..44387
                     /locus_tag="KQD82_000033"
     CDS             42210..44387
                     /locus_tag="KQD82_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG4378541.1"
                     /translation="MQSTYNALEYNKLISFIWSVADDCLRDVYVRGKYRDVILPMTVI
                     RRFDSIIEPEKANIMKVKEMAEKQGWDVAKTLDTAVGLPFYNTSNFCLKDLKHETNRQ
                     NLKKNFEEYLNGFSENVKEILQKFDFNNQLTKMTDAGILGSVIEKFTSSELNLSPYDE
                     KNSNGEIIKKGLDNHAMGTLFEEIIRKFNEENNEEAGEHFTPRDVIELMADITMYPIM
                     DKIKDGTYSIYDGACGTLGMGTVAEERLKDFAKENGKEVSIHLIGQEVNPETYAISKA
                     DLLIKGGDTDSNNVHYGSTLSEDKTSGQHFDFMLSNPPYGKSWKMDLAILGIGEDKDL
                     KKNIIDKRFVRNYKEQNDFRMIPDVSDGQLLFLLNNISKMKETELGSRIVEVHNGSAL
                     FTGDAGNGASNARRFMIEEDLIEAIIQLPENMFYNTGITTYIWVLSNRKEERRKGKIQ
                     LINANGIKTSLRKNMGKKNCEFSEADRRFILNEYLKFEENEYSKIFSNEEFGYFKVTV
                     ERPLRQAVLCNYENINEVEKELEKIGATTGKIDKKLIAESFVKGTAGSIKELEKKENI
                     KAYLEVLREMESSEKYLDYEAFEKAFNKNLKNKNIKGASFSKLVSTGLLANMIIRDEE
                     AEVQTDSKGNLIVDPELRDTESIPMTFVGGIDEFIRQEVLPYHEDAFVDESKTQIGYE
                     INFTKYFYKAKKLENVEDIVCRIKELEKRSDGMMETVLEGLYE"
     gene            44380..45741
                     /locus_tag="KQD82_000034"
     CDS             44380..45741
                     /locus_tag="KQD82_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG4378542.1"
                     /translation="MNKYERYRDTGLIWINKVPKKWNLQKINAVFDERREKVSDKDYE
                     ALSVTKNGIFKQLDNVAKTIDGDNRKKVKINDFVINSRSDRKGSSGLSRFDGSVSLIN
                     TVLKIKKEYPRYMHYLLKSVPFQEEFYRNGKGIVADLWSTNFQSMKSIILPIPPIEEQ
                     VQIANYLDWKINEINRLIQIEKEKIKELETLRFNAISEFVLRGIGTQNYKKSSINWLD
                     EIPSHWNEVSIRWCVNIIRGNSTFTKDDLQNRGKYVGLQYGKVYKTEIIDSEFDFYVS
                     DKFYKPAQVVNRNDIIIVSTSETVEDLGHTSFYDRDDIGLIGGEQILLKPSNNINSKY
                     LFYLSKIFRMQLQLCATGIKVYRFKISDLKQIYVPLPPMKEQEKIVSNIELKLEQIDE
                     RVKNNYAFIKELELLKQSLISEVVTGKIDVRNIDISEYEKVTIIEDEIEEFDEVGGEE
                     DGN"
     gene            45731..48649
                     /locus_tag="KQD82_000035"
     CDS             45731..48649
                     /locus_tag="KQD82_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBG4378543.1"
                     /translation="MGIRDTKMEAELENNIIEYLTREQKYGAYQYITPDEMKLSYNRK
                     YAFDEIRLLTFIKESQPGEFDILRLDTEDGKDKFFKQLDVSIKQKGIVAVLKNGIKCY
                     PSSGTIIFYHALDPKRPSSYDEFRENIFTVTNQLTYSDKNRGLELDLAIFVNGLPIVT
                     MELKSRASSNGWTYKDAEEQYKNDRDPKETIFSFKRCIAHLAVDENFISFTTRLDGKN
                     TRFMPFNKGTIDGGSGNPINPDGTMTDYLWKNFLDKEILTSLIRDFAYISVDKVKKSE
                     TLIFPRYHQYRVVTRLVDDVQKNGVGNRYLIQHSAGSGKSNSITWLAYRLVEVDYNNQ
                     KAFDSVIVVTDRLNLDKQINDNIRKFIDEKSVVGHANSSTDLKNLLVDGKKIIITTVQ
                     KFPYLLEKIGTDLKGKNFAIIIDEAHSSQSGRAAASLNMAVSGSLSNDDEFEIEDKLN
                     EFIEARKMPENASFFAFTATPKAKTIQMFGNVFDLYSMKQAIEEGFILDVLKNYTHYE
                     NYYKIYKTIEDNPNFDKKKAQKKIRNFVEGQEFPIREKAEVMVNHFLTNTVNKINGKA
                     KAMIITQSILRAIEYYYIVSELLNNSNTGYEALVAFSGEKEYKGKVVTEASLNGFSDK
                     ETPEKFKQDKYKFLIVADKYQTGYDEPLLHTMYVDKVLNDVKAVQTLSRLNRSAKYKI
                     DTCVIDFANEPEHILEAFQPYYKETKLEGETDPNKLNNLLSMLDSKYIYEEDEVDRLV
                     DLFLKNSPRSFIDSIVDKSVERYKVLSEEEQVEFKSGVKSFIRTYNFLASILPIGQVD
                     WEKKVIFFEQLIHRLPTPEGEDLSEGILESVDLESYRLEKKNTIDIILEDEDGIVEGL
                     GISAGKKNEVELDSLENIVSTFNDIFGNIDWQDKDNVARQIKELPEMVMKNEKFKNAL
                     KNSDIENIKREYHSALKEVFRIIMVDNMELFGQWTNNSNFSKWLNDTIFDEIMKKNKR
                     "
     gene            48664..49938
                     /locus_tag="KQD82_000036"
     CDS             48664..49938
                     /locus_tag="KQD82_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378544.1"
                     /translation="MGSISGVTRRDIIDLFKNGITEDNWLTEETIYYPYYGRFDIVDF
                     LKRLYKLDTWKSGDSRLKNAEQEITMHTRNGDYPDDWIFADERFHLLDGEDSVLLGFL
                     CEVFHPEVRDENKEWKKYLERINSLLREDGYELIASSRLSGRDVFTWRVYIKKPDMYI
                     PFSERNKSLIVGRKINFKLPNAVRHQLFKVMDEYDEIFYLTDETNWNYTKYSSDYVLE
                     DINKFYVPKHYVDSNLAEIKKFKDFQEGTSPFVIFDVIESFNRHTTNSEGFENEINSI
                     FNLNNINVELRRGEIHSSTNKTLSLDPSVKINEIGIEELIRAAEDLYRKGKYSYAVEK
                     IWDAFERIKTYYYPELDKKKSANKIIDELSNGNDSIKSMFQAEFKYLTDIGNSYRIRH
                     HEKNKIDITEDLHYEYFYKRCLALISVLVKKI"
     gene            49956..52493
                     /locus_tag="KQD82_000037"
     CDS             49956..52493
                     /locus_tag="KQD82_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG4378545.1"
                     /translation="MVDFKKKVALKSKAKVTNPIDLYNTLDRKSIAGPLRPVQEYVLN
                     EWYENRRNERDLIVKLHTGEGKTLIGLLILQSSINIGEGPCIYVCPNIYLVRQVCAEA
                     NKFGIPFCEIEQNNVLPNEFLSGEKILITHAHKIFNGKSIFGIGNKCVNAGTIILDDS
                     HACIDVLKDAFTITIGKKEKENVYNKILTLFSEDLIEQGEGSFLDIKSGEYETFMTVP
                     YWAWINKKSEILKILSDESASDEIKFAWPLMKDKIIEYSCFISGNKIEITPYNVSVEQ
                     FRTFSSAKRRILMSATTQEDIFFVKGLDFSTNAVANPIKYPKSRWSGEKMVLMPSLID
                     EECDRDLVATQFAALTNAPFGVVAIVPNTKRAVYYENIGANFPKNNKELFEVIDDLRK
                     GKFSNTVVINNRYDGIDLPDESCRVLVIDSLPYLNSYFDKYEEKCCPNSEIINKKIAQ
                     KIEQGIGRAVRGEKDYCAIIVIGSDIEKFMRGILTRKYFSSQTQKQIEIGFEVAKMAK
                     EEILQDDPSMKQIFSLIKQILNRDEGWKEYYASEMNEIEDGVLDTSIYERYAAETKLE
                     KLFSDGEYEKASEETQKFIDKFIHDDLERGWYLEQKARYTFMYSQEESAHLQKVAFQK
                     NSQLLKPNSGIIYDKVSFLNENRLIRFRKYISKFNDFDEFSLHLNEVLNNFSFGIDAD
                     KFESALKSIGEMLGYISQRPDQEIRKGPDNLWCASNKKYAIFECKSEVDENRGAIKKS
                     EAGQFNNHCGWFKTEYDEYVDVLRVIVIPTKKLARDADFTDEVFVMRKNGVKKLKDNI
                     KNFMKEIKKYEIESITDTKLQKYFDMYNLNIDNLIGNYVEEIYHLEK"
     gene            complement(52510..53841)
                     /locus_tag="KQD82_000038"
     CDS             complement(52510..53841)
                     /locus_tag="KQD82_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG4378546.1"
                     /translation="MAITKIHPIKSTLNLAIAYITNEEKTDEKILISTNNCFTSTAHT
                     QFLKTREDNNVKGTVLARHLIQSFLPGEATPEMAHKVGMELCKKILKDQYEFVLTTHI
                     DKGHIHNHIIFNNVNMITGKCYQSNKRSYHKIRYQSDKLCKENKLSVIDEFYETYKKK
                     YKTNGKSWYENEQFKKGTSWKSRLQFDIDRTIKQSKDWDEFLKKMAAIDYEIKYGKHI
                     AFKHKDKERFTRAKTIGDDYTEDKLKERITKNANQKTYTVKKRVGNIIDIANNEKVKS
                     SKGYEYWATKHNLQVATNMVVLMREKGFKSISQLDDFIKESADKRQNLQDEIKILDSK
                     ISALSSTMEQVHTVKIYRQIYQGYKKEPSDKGFFDEHKAEITLYQNALSDLKKSYSKL
                     PNSKDILAALDSLHEKKNTLMQEYSSTKSDMKELYQIRKNYEKYMGKEMER"
     gene            complement(53842..54201)
                     /gene="mobC"
                     /locus_tag="KQD82_000039"
     CDS             complement(53842..54201)
                     /gene="mobC"
                     /locus_tag="KQD82_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG4378547.1"
                     /translation="MANRVRNVQLKINLTEEEKAFFEKKMKMAKCKTMSLFLRKLVSE
                     SDIYVVDLQPFREIQGLLFRYASSVNQIAKRVNSTGVIYSDDIKDMKSQIEGLSKEIW
                     QIHSLLLNRTTDKGDEV"
     gene            54535..55692
                     /locus_tag="KQD82_000040"
     CDS             54535..55692
                     /locus_tag="KQD82_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG4378548.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAFLTFNKMTEEQKNAVINKKGDGYEKWKNGLKRIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGSESNLLDEFKKTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFDKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNMVGVVTDTVDDNGENKEAIEKSKLIQKKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDRNGNIVGDTYSGAKSAKEWKQVIEKELAKIKNK"
     gene            55714..55866
                     /locus_tag="KQD82_000041"
     CDS             55714..55866
                     /locus_tag="KQD82_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378549.1"
                     /translation="MVNKFLKSRTLSLVIVFISMAMTYYGHSHGEARVVLEKAIRICM
                     ECVGIG"
     gene            55859..56749
                     /locus_tag="KQD82_000042"
     CDS             55859..56749
                     /locus_tag="KQD82_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4378550.1"
                     /translation="MDRIKKIINGSTKKDKYKIRDRFRHLFQSVWFLVTNSYFEGFKT
                     GKIYGGDLKEVCVPGMNCYSCPGAKGSCPIGSLQAVIGNSNYKFSYYIVGFMFLIGAL
                     IGRFVCGWLCPFGLIQDLLHKIPFFKKIETFKLDKHLRKLKYIILIVFVIILPLFLVD
                     ILGQGSPYFCKLICPVGMLEGGIPLVLLNKSMRGAIGFLYYWKGVILILTLLLSIVIY
                     RPFCKYVCPLGAIYSVFNPISVFRYRLDEEKCISCGRCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISCGIKK"
     gene            56803..58080
                     /locus_tag="KQD82_000043"
     CDS             56803..58080
                     /locus_tag="KQD82_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378551.1"
                     /translation="MIKNAIAYITRKKNRTFIIFVILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDKFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSLESTDLALNKLKELKIDESKYSIEKDSNVFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLLLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGE
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            58090..58746
                     /locus_tag="KQD82_000044"
     CDS             58090..58746
                     /locus_tag="KQD82_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378552.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLFAGLDKPQTGKILFKNEDIEKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADAADIILELSGKKLKKVNKMNLEVE
                     "
     gene            58746..60122
                     /locus_tag="KQD82_000045"
     CDS             58746..60122
                     /locus_tag="KQD82_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378553.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFFMEINRQVNPGTPRGGGNVKGEDIKKISQTDSVDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     VQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTAGKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            60220..60873
                     /locus_tag="KQD82_000046"
     CDS             60220..60873
                     /locus_tag="KQD82_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4378554.1"
                     /translation="MKILIVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSAI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKVRIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELDVLKCLLD
                     NDGQVLTRMQIIDYVWKDSEEIPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLEIK"
     gene            60878..62200
                     /locus_tag="KQD82_000047"
     CDS             60878..62200
                     /locus_tag="KQD82_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4378555.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEE
                     IHNKANEISSNMNGKEIKYIEQTLDLYSKSSEIKAFIKEKKNNNNEIQIKDNIDANLE
                     SNSNSLIIEEREIKLNDGKKTYLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIIS
                     LIYAKLIKNNIQEIKIVTDKMMKLDKKMRLKVSSNDEVGELKQQINDLYSTLLRTIDD
                     LEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECI
                     EIVDSLTKNISQILSVHSIENLNNDEEYLKINDTIEDILKKYEILANQKKITVNNYIL
                     DENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKI
                     FDVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            62724..63134
                     /locus_tag="KQD82_000048"
     CDS             62724..63134
                     /locus_tag="KQD82_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4378556.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            63623..63775
                     /locus_tag="KQD82_000049"
     CDS             63623..63775
                     /locus_tag="KQD82_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378557.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            63876..65552
                     /locus_tag="KQD82_000050"
     CDS             63876..65552
                     /locus_tag="KQD82_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG4378558.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMMQEK
                     INMKVDTSAIENEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDKDLNISLDNDKQVETVAKIIKQ"
     gene            complement(66030..66668)
                     /locus_tag="KQD82_000051"
     CDS             complement(66030..66668)
                     /locus_tag="KQD82_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378559.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIDVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(67078..67254)
                     /locus_tag="KQD82_000052"
     CDS             complement(67078..67254)
                     /locus_tag="KQD82_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378560.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(67308..67404)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            68200..68601
                     /locus_tag="KQD82_000053"
     CDS             68200..68601
                     /locus_tag="KQD82_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG4378561.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            68598..69665
                     /gene="rsgA"
                     /locus_tag="KQD82_000054"
     CDS             68598..69665
                     /gene="rsgA"
                     /locus_tag="KQD82_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG4378562.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            70201..70899
                     /locus_tag="KQD82_000055"
     CDS             70201..70899
                     /locus_tag="KQD82_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378563.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINENMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYAKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            71077..71967
                     /locus_tag="KQD82_000056"
     CDS             71077..71967
                     /locus_tag="KQD82_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378564.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMNSLFILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(72368..73324)
                     /locus_tag="KQD82_000057"
     CDS             complement(72368..73324)
                     /locus_tag="KQD82_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4378565.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            74027..75022
                     /locus_tag="KQD82_000058"
     CDS             74027..75022
                     /locus_tag="KQD82_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4378566.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIVPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            75000..75863
                     /locus_tag="KQD82_000059"
     CDS             75000..75863
                     /locus_tag="KQD82_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG4378567.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTLVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            75860..76654
                     /locus_tag="KQD82_000060"
     CDS             75860..76654
                     /locus_tag="KQD82_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG4378568.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            76939..78318
                     /locus_tag="KQD82_000061"
     CDS             76939..78318
                     /locus_tag="KQD82_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4378569.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVETFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            78766..79479
                     /locus_tag="KQD82_000062"
     CDS             78766..79479
                     /locus_tag="KQD82_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4378570.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAKNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(79751..81289)
                     /locus_tag="KQD82_000063"
     CDS             complement(79751..81289)
                     /locus_tag="KQD82_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG4378571.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENGQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            82171..82626
                     /locus_tag="KQD82_000064"
     CDS             82171..82626
                     /locus_tag="KQD82_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG4378572.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            82675..83259
                     /locus_tag="KQD82_000065"
     CDS             82675..83259
                     /locus_tag="KQD82_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG4378573.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(83264..83587)
                     /locus_tag="KQD82_000066"
     CDS             complement(83264..83587)
                     /locus_tag="KQD82_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378574.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            83739..84257
                     /locus_tag="KQD82_000067"
     CDS             83739..84257
                     /locus_tag="KQD82_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4378575.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(84433..85101)
                     /locus_tag="KQD82_000068"
     CDS             complement(84433..85101)
                     /locus_tag="KQD82_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378576.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            85216..86631
                     /locus_tag="KQD82_000069"
     CDS             85216..86631
                     /locus_tag="KQD82_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4378577.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKEFSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            86854..87312
                     /locus_tag="KQD82_000070"
     CDS             86854..87312
                     /locus_tag="KQD82_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378578.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            87309..87893
                     /gene="clpP"
                     /locus_tag="KQD82_000071"
     CDS             87309..87893
                     /gene="clpP"
                     /locus_tag="KQD82_000071"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG4378579.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            88205..89281
                     /locus_tag="KQD82_000072"
     CDS             88205..89281
                     /locus_tag="KQD82_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4378580.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            89348..91411
                     /gene="bclA3"
                     /locus_tag="KQD82_000073"
     CDS             89348..91411
                     /gene="bclA3"
                     /locus_tag="KQD82_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG4378581.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGNTGADGATGPTGATGATGV
                     AGATGATGPTGATGNTGADGATGPTGATGATGADGATGPTGATGATGVTGATGPTGAT
                     GATGATGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAA
                     GTLTNFAFSMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGA
                     TVTLAPPLTGILSVGSISSGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASA
                     GIAIN"
     gene            91580..92341
                     /locus_tag="KQD82_000074"
     CDS             91580..92341
                     /locus_tag="KQD82_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG4378582.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            92650..92928
                     /locus_tag="KQD82_000075"
     CDS             92650..92928
                     /locus_tag="KQD82_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378583.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            93221..93796
                     /locus_tag="KQD82_000076"
     CDS             93221..93796
                     /locus_tag="KQD82_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG4378584.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            94235..94945
                     /locus_tag="KQD82_000077"
     CDS             94235..94945
                     /locus_tag="KQD82_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4378585.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            94939..96255
                     /locus_tag="KQD82_000078"
     CDS             94939..96255
                     /locus_tag="KQD82_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG4378586.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDNKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            96267..96407
                     /locus_tag="KQD82_000079"
     CDS             96267..96407
                     /locus_tag="KQD82_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG4378587.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            96466..97043
                     /locus_tag="KQD82_000080"
                     /pseudo
     CDS             96466..97043
                     /locus_tag="KQD82_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
     gene            97300..97674
                     /locus_tag="KQD82_000081"
     CDS             97300..97674
                     /locus_tag="KQD82_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378588.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            97757..99466
                     /locus_tag="KQD82_000082"
     CDS             97757..99466
                     /locus_tag="KQD82_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378589.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITVIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNIKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSYMAYKINLSSKSNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            99481..100353
                     /locus_tag="KQD82_000083"
     CDS             99481..100353
                     /locus_tag="KQD82_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4378590.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            100354..101142
                     /locus_tag="KQD82_000084"
     CDS             100354..101142
                     /locus_tag="KQD82_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378591.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(101238..101876)
                     /locus_tag="KQD82_000085"
     CDS             complement(101238..101876)
                     /locus_tag="KQD82_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG4378592.1"
                     /translation="MIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQ
                     KTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDK
                     AVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCAKKNNRFL
                     EALKILESKMIGNKIVVESVVPKLSKLSFCKKGESSEIAKTHYYEILKNLEK"
     gene            complement(101903..102532)
                     /locus_tag="KQD82_000086"
     CDS             complement(101903..102532)
                     /locus_tag="KQD82_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG4378593.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPNGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            102843..103502
                     /gene="cprR"
                     /locus_tag="KQD82_000087"
     CDS             102843..103502
                     /gene="cprR"
                     /locus_tag="KQD82_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG4378594.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT        39905 a        12192 c        19186 g        32263 t
ORIGIN      
        1 acaatgaata atcaagatat atatataaat ttattaaata tattaagtaa agaggatata
       61 aaagtcgatg aacctatgaa aaaacatatt tcatttaggg taggaggacc agcagatata
      121 ttagttagac ctagaactga agaacaatta aaaaatgtac ttaagttggt aaaaaaagaa
      181 tctattccgt atttaataat tggaaatggt tcaaacattt taattaaaga tggtggaata
      241 agaggagtgg taatagaatt agctgataat ttcaattctt atgagataaa tgatactagg
      301 atgactgctc agtcaggagc tttattatca gttttaggga aagctctcca aaagcaagaa
      361 ttaaaagggt ttgagtttgc atcaggaata cctggcacat tgggaggagc attagccatg
      421 aatgctggag catatggtgg tgaaatgaag gacatagtga agtcagtaag actgatggat
      481 atggaaggca atatatttga attatcaaat gaacagatgg aatttgggta tagaaaaagt
      541 ataatatcga aaaacggata tatagcttta tcagcagaat tagagttaca agaaggaaat
      601 tatgatgaaa taaaaagttt aatggatgat ttagcgacga gaagaataac taaacagcca
      661 ttaaattttg ctagtgcagg aagtacattt aagagaccaa caggatattt tgcaggtaaa
      721 ttaatagaag aaactggatt gagaggtctt actttaagag gagcacaagt atctgagaag
      781 cattgtggat ttgtagtcaa tcaaggagaa gcaagtgcta aagatatttt ggatttaata
      841 tatgttataa aaagtgctgt atatgccaag tttggggtta tgttggaaga agaagtcaaa
      901 atacttggag aggattaaca aatgaaaata ctggctgatt atcatacaca tacattatat
      961 agtcatggca aagggactat tgaggataat gtaaaggtag ccatttctaa aggaataaaa
     1021 acaataggaa tatctgacca tagttataaa catattgcat atggtgtaaa gaaaaaagat
     1081 attctcaaaa tgagagaaga agtagattat ttaaatgata agtacactga tataaatata
     1141 cttttaggta tggagtgtaa catattagat gataaaggaa atatagatat ggatgataaa
     1201 tttattagag attttgatta tattatggct ggatatcact ttggttctac tccaacatca
     1261 ttaagaagct tattaaatca ttgtaataac tatattttta aaactgaaaa agcaaaagaa
     1321 tataatacaa aagctgtaat aaatgcgata agtaataatg atatttttat aattactcat
     1381 ccaggagata aaggcgatgt gtatatagaa gaagtagcaa agacagcaaa aaagacaaat
     1441 actagacttg agataaatag tagccattct tttttgaatg taaatcaatt aaataaaata
     1501 aaagggctag aaaataaatt tataataggc tcagatgcac attgtccaga aagagttggt
     1561 gattttgaac tagctatgaa gactatatct gactctaatc tggatatatc gttagtagaa
     1621 aacattataa tataataaac ataagataaa agggaggagt tagcaatgaa atttgttata
     1681 gttacaggtc tttcaggatc tggaaaaagt gagacaatga gagctttaga ggatatggga
     1741 ttttattgtg tagataatct acctcctgct ttgataacta aatttgcaga attatgttat
     1801 caaccaaatt caagtattga taaagttgct ctagggatag atataagagg gagaaagttt
     1861 tttgaagcgc ttcatgaaag cttaaattat ttagaaaaag aaaattatga atatgaaatg
     1921 gtatatttgg attgtaatga tgatgtattg ttaaagagat ataaaatgac aagaagaaat
     1981 catccattgg caaaagatat gcagattcca gaaggtataa aaatggaaag aaagataatg
     2041 gaacctttaa aggagttatc tacatgtata attgatacaa ctaacatgaa acctaaagac
     2101 cttaaggaag aaataaagaa aatatattct tctggtgaag ataatccaaa cctaactatt
     2161 tctgtagtat cttttggatt taaacatggt atacttgctg atgcagattt agtatttgat
     2221 gtaagatttc ttccaaatcc ctattatgtt gaagagttaa gagctaaaac tggtgatgat
     2281 aaagaagtta gagattatgt tatgaattct aaaatcagtg aagaatttta tgtgaaactt
     2341 ttagatatga ttcatttttt agtacctcaa tatatagagg aaggaaaaca acatctagta
     2401 attggtgttg gatgcacagg tggaagacat aggtcagtta ctataacaaa tcttatagcg
     2461 gaggatttat ctaataaggg atatagagtt gtaaagaaac atagagactc tatgttgcga
     2521 taatggaggc aaacatggct aaattactta ttattgtagg aattttaggt tggattgcat
     2581 taattgtatc gttatttata tataaaaaag caaaaaatga aaaagtaaaa ctatttcaaa
     2641 ggtctattgt tgaaaataga gaaccaaatg tggttgtgat aggtggaggt actggtcaat
     2701 cggtattttt aagaggcctt aaacacacta cgcaaaatat aactgcaatt gtaactgttg
     2761 ctgatgatgg tggaggttca ggtgtattga gagaagactt aggaatgctc ccaccgggag
     2821 acataagaaa ctgtttatta gcacttgcaa atatagagcc aacaatgaat gaagttatgc
     2881 aatataggtt tacagaaggg cttttaaaag gtcagagttt tggaaatttg tttttggctg
     2941 ctatgaatgg gttgtatgga aactttgaaa aagctgtata taaattaagt gaaatttttg
     3001 ctataacagg aagagtttta ccagtaactt tggaagatgt aaatttagtt gccaaattag
     3061 aaaatggcaa tataataaat ggagaatcta gtatacctga agagtcaaaa attcaaaaaa
     3121 gctctattga taaaattttt ttaaatccaa aagatgtaaa accattaaag gatgttattg
     3181 catctattta tgatgctgac attataatta tgggtcctgg aagtttatat acaagtataa
     3241 tacccaattt acttgtagag gggattgtag acgctattaa atcgtctgta gctccaaaag
     3301 tatatatagc aaatataatg actcaaccag gtgagacaga agggtacaat gtattagagc
     3361 atgttaatgc tatagtaaaa cacactgatg agaatttaat agattatgta atcgcaaata
     3421 atgagatatt gccagaagaa atgcttgact tatataaaca agatggagca gagcaagtct
     3481 tgttagataa aaagcaaaaa gataaattaa aagagatggg tataaaaact gtagagaaga
     3541 atttaattga aataaaaaat aattatataa gacatgatgc aaagtatata tctaacatag
     3601 ttattgagtt agccctaaat cataattaca acaaaagtta gtaaaaaagg attttaaaaa
     3661 attgtgtaga aatccttatc attatgttag ttttaaggag tgaacatcga tgagagaaga
     3721 ggttagtgct ggtggtgtag tcctgtttgg caatacgatt cttttactaa ggaagttcaa
     3781 tggagattgg gtgttaccca agggaaaagt agaagaaggg gaaaataacc aagaggcagc
     3841 attacgtgaa gtaagtgaag aaactggagt aaaagcagat atattgaaat atttaggtga
     3901 aatacactat acattcaaag aaaactggga tgaaaataga gcagttcata aaacagtttt
     3961 ttggtatttg atgcaagcaa agaatatgga tactattcca caaaaagagg aaggctttat
     4021 tgatgctaaa tttatccacc tagatagagt agtagactta gctaggtatg atgatgaaaa
     4081 ggagataata aaggttgcct tacaagaaat aaaaaaaagg ttaaagaaaa attagttaat
     4141 aggtgataga tatgtctttt tcaactgaaa ccaaaaatga actagcaaga gttatatctg
     4201 ggaatgaatg ttgtaatata gctgaattgt cagctctagt taaatcaggg ggaagtatac
     4261 aaattgttgg atataagaag ctaaatctaa agataacaac agagttaaat tcaatagccc
     4321 gtaaagtatt taaattatta aaaaagaatt ttaatattaa tactacaatc tcagtaaata
     4381 agaatcaaat gctaaagaga aataacagtt atgtattaat ggtaacaagc gaaatgggtt
     4441 cagagatgtt attaaaaaag ttaggtatac ttgaagataa agaaggattt tttactataa
     4501 acaaaatttc agagaactta attaagcatg aaggatgtaa gagagctttc ataagaggag
     4561 cttttcttgg tggaggttct ataagcgacc cagaaaagaa ctatcatatg gaatttgtaa
     4621 ctaataatga ggagtttgca gattctctaa aagaacttat aaactcttta ggttttaata
     4681 gtaaaattgt ggctagaaaa aataatcacg ttgtttatat caaagagagt gaacaaatat
     4741 cagatttgtt gagtattata ggaggacacc acgctctttt aagtcttcaa aatactaaaa
     4801 tagtaaaaga aatgagaaat aatgtaaaca ggatagtaaa ttgtgagaca gctaaccttt
     4861 ctaaaacagt aaatgcagca gtaagacaag tagaaaatat taagctcata caagagacta
     4921 taggaattag cagtttgcca gaaaatttac aagagatagc taagattaga atcgagtatg
     4981 aggatatgac tctcaaagaa cttggagaga tgttagaacc accaatagga aaatctggag
     5041 tcaatcatag acttagaaaa atagaagaaa ttgcaacaga tttgaaaaaa aaatcatagc
     5101 aataaattga ggggacaatc tcagaataag cattaattta taacatataa tggtatacgt
     5161 tgaagttaat tcctattttg agataaaagt ctctgttttt gttttaggac atatatttgt
     5221 ggtaaaatta aaattataaa gcaggaggtg atttaatgaa caaagatttt gctcatcttc
     5281 atgtacatac tgagtatagt ttacttgatg ggttttcgag ggtgaaaaaa cttataaaaa
     5341 gggctaagga actaaacatg agctcaatag ctataactga ccatggttgt atgtttggtg
     5401 ttatagattt ctacaaaaca gctataaaag aaggaataaa gccaattata gggtgcgaag
     5461 tctatacagc tgcacgtgga ttgagagata aagacccaaa ctatgacaaa tatcaaggac
     5521 atttagttct gttagctaag aatatggaag gatataaaaa tttaataaaa atagtttcaa
     5581 cttcatacgt agaaggtttt tactataagc ctagagttga catggaagaa cttaaaaagc
     5641 ataacaaagg gattatagca ctttctgcat gccttgcagg agatgtagca cgtgctttaa
     5701 tgaatagaaa ttatgaaaaa gcaaaacaat ttgctcttga ctacagagac atatttggag
     5761 aagaaaattt ctttttggag attcaagatc acaatttacc tgagcaaaaa gaagtaaact
     5821 ctggtttagt aaaattatca aaagagactg gtataccact agttgctact aatgatgttc
     5881 actatgtaaa taaagaagat tcgaaaatac atgatgttct tatgtgtatt caaatgggaa
     5941 aaacattaaa tgacccaaat agaatgagat ttggtagtga tgagttttat ttaaaatcaa
     6001 gagaagaaat ggaagaatta tttccatatg cattagaggc tattgacaat actgtgaaaa
     6061 tagctgatat gtgtaatgtg gagtttgatt ttaatactat acatcttcct aagtatgatg
     6121 ttccagaagg atatacacca gaaacttatt taagggagtt gtgttttaat ggattaaaag
     6181 agagatatga taatcctagc aatgagatac tggagagatt aaattatgaa ttagatgtaa
     6241 ttgaaaaaat gggttatgta gagtattttt taattgtttg ggattttata aatttttcta
     6301 aagaaaataa tattatagta ggaccaggta gaggcagtgc ggctggttct atagtagcat
     6361 atacacttaa aataactgac atagacccaa taaagtactc tctattattt gagcgttttt
     6421 taaatccaga acgtatatcc atgccagata tagatataga tttttgttat gagagaagag
     6481 aagaagttat agattatgta aagagaaaat atggtgatga ccatgtagcc cagataataa
     6541 cttttggtac aatgggtgca aaagctgcaa taagagatgt tggaagggta ttggatatag
     6601 gctacaataa agttgacaag gtagccaagg aaataccatt tgctttagga atgacaatag
     6661 ataaggccct agatacaaat cctaatctta aagaattgta tgaccaagac ccggaaacaa
     6721 aagaaataat aaatatatca aaacagatag aaggaatgct aagacatgct tctactcatg
     6781 cagcaggagt tgttatatca aaaaatcccg tagatgaata tgtacctttg tacaaacatc
     6841 aagatgcaat cacaacacag tttactatga caacacttga agaacttggg cttttaaaga
     6901 tggattttct tgggctaaga actcttacag ttataagaga tactcttgat ttaatagaaa
     6961 aaaatagagt tataaaaaac tacacagaaa aaatagactt ttcaaagatg gattatgacg
     7021 acccgaaagt atatgaaatg ctttcatcag gaaatacact aggggtgttt caattggaga
     7081 gtgctggcat gagaagtttt atgaaacagc taaaaccaga caattttgaa gatattgtag
     7141 cgggaatatc cctatttaga ccaggtccaa tggattctat acctgcatat ataaaaaata
     7201 aaagcaaccc aaaagatgta acatatctac atgaaaaatt aaagcctata atggaagtta
     7261 catatggttg tttggtatat caagagcagg ttatgcaggt cgttagagat ttaggtggtt
     7321 atagttatgg acgaagtgac ctagttagaa gagccatgag taagaaaaag atggacgtga
     7381 tggaagaaga aagacaatat tttattcatg gtaaatttga tgaagatggt aatatagaaa
     7441 ttgcagggtg tatcagaaat ggtgtagaag aggaaatagc taataaaata tttgatgaca
     7501 tgatagactt cgctagatat gcatttaata aatcacatgc agctgcatat ggggttttag
     7561 catatgagac agcttatcta aaaacatatt atccagttga atttatggca gcattgataa
     7621 ccagtgtaat gggaaatact gataaggtag ttgagtatat aagagaatgt aaagcactag
     7681 aaatagatgt attacctcca gatataaaca aaagtttttc aaagttttca gttgaaggcg
     7741 acaatataag atttggtcta gcagctgtaa aaaatgttgg tgtaaatata ataaacaata
     7801 taatcgctga aagagaatct aatggattat ttgtagattt agtagattta gtcaaaagat
     7861 tagaccaaaa agatacaaat aagagagtaa tagaaagtct aataaaatgt ggagcttttg
     7921 ataatataag tgaaaataga gctagtctga tggctggtta tgaaagttta ttagaaagtg
     7981 tatctatgga tagaaagaaa aatgttcaag gtcaaatttc tttatttgat gcttttggag
     8041 atagtgagga agatagtgat tttcaacaaa tatacagttt gcctaaaagg caagagtacg
     8101 aagaaagaga gagacttaac cttgaaaaag aagtcttggg aatgtatgta agtggacatc
     8161 ctttatcaca atttgaaaaa gaactacaag aaaaaacatc tatggataat ggaaaattaa
     8221 attcattaaa agaggaagaa gagtcatacc tacaaatgaa tgaaacagat gttataatgg
     8281 gtggaatgat tgtaactaaa actattaaaa caacaaaacg aaatgaaatt atggcattta
     8341 ttgaattaga agatttgtat ggtgctatag aaataatagt ttttcctcaa ttattacaaa
     8401 agtataatgt cattttgaat gaagataata taatatatgt aaaaggaaca ttaagtataa
     8461 aagaaggcga aaatgcaaaa cttatagcta gagagataaa agatataaat gatgatactg
     8521 actttgcaga tagaaactac aattcaggaa gaactagaaa tgtagaaaat attaagccag
     8581 aaagtggtaa aaaattgtat ttgaaaatag atagtatgtc tgatactgat acaactaata
     8641 atattgtaga aatagccaaa caatatccag gtaatgacag tatatacttg taccctatgg
     8701 atgaaaatat aaatggtaaa cgtaaagcat ataaaatgac cggggcattg acttgtatat
     8761 gtgatgaatt tataaataac ttagaagagt tacttcctaa agataacata aaagtaaaag
     8821 cttgattttt tatataaaaa tctaaatata ttataataat atgaaaaata ttgtccgttt
     8881 tgtgatataa tatcacaaaa tggacattta aggacattta atttttttag tcttgatggg
     8941 attaaaaagt gatataggga caaaaaaagt ccattagaat acaataacaa tgattataat
     9001 aaaaaattga cataaaatcc aaggaggtaa aaaatgaaaa ctataggatt actaacaagt
     9061 ggaggagatg ctccaggaat gaatgctgcg attagagcgg ttgtaagatc agcaatatat
     9121 tatggatgca aagtatatgg aatcaataga ggatataagg gattactaga agaagattta
     9181 actgaaatga acttatcttc tgttggagat attatacaca ggggaggaac tatattaaaa
     9241 tcttccagat gtgaagaatt taaaacagaa gaaggtagat taaaagcagt aaaaattctt
     9301 aaaaagtata aaattgattg cttagttgta attggtggag atggttcatt tgcaggagcc
     9361 caaaaactta gtgacttagg attcccagct ataggcatac caggaactat agataacgat
     9421 ttagcatata cagattatac tataggattt gatactgcaa tgaatactat tatagatgct
     9481 ataggtaaga taagagatac atcatcatct catgaaagag ttaacatagt agaagttatg
     9541 ggaagacact gtggagattt agccttatat gcaggtcttg caggaggagc ggaaactata
     9601 atagtaccag aagtagaaat tacagtagat gaagttgctc taagattaaa gactactcaa
     9661 aaaagaggta agagacatag tataatcgtt ttagcagaag gagtcggaag tgctagtgat
     9721 ttagaaaagg aacttaaaaa agagtcagga gcagatttaa gagtcactgt attggggcat
     9781 gtccaaagag gaggaagccc aactgtatct gatagaatac tagcaagtag acttggagta
     9841 agagcagtag aattattatt agatggaaaa tcagctagag ttgtaggaat aaaagaaaac
     9901 aaaataattg atttagaaat aagtgaagct ttagctcaaa agaaagtatt tgataaagaa
     9961 gcttatgaga tggcaaaaat attatctata taagttttag tattaaaaat aaatctgtgg
    10021 aataatatct caggaggagc aaatatgtta aataatgtaa agaagacaaa aattgtttgt
    10081 acactaggtc cagcatcaca aagtgaggaa gttttaactc aacttatgca aaatggtcta
    10141 aatgtgtgta gatttaactt ttctcacggt tcgcatgaag aacataaaga aagaattgat
    10201 atggcaaaga aagttagaga aaaattaaat aagccagttg ctatattatt agatactaaa
    10261 ggtccagaaa ttagaacagg taactttgaa gacccagagg tatttttaga agaagggcaa
    10321 aagttcacta taactatgaa agatattatg gggactaaag aaatgtgtac agtaagctat
    10381 aaaggtcttg ctgaggacgt taaatcagga gattctatac taatagatga tggtttagtt
    10441 ggcttaagag taaaagaaat aaatggagaa gatatagttt gtgttgttga aaattcagga
    10501 atagttaaaa atcataaagg agtaaatgta cctggtgtta agataaattt accagcaata
    10561 actcctaaag atataagtga tatagagttt ggaataagtc aaggaataga ttatatagct
    10621 gcttcttttg taagaaaggc atctgatgta ctagcaataa gagaagtatt agagaacaat
    10681 aatgcaactg atatacaaat aatatctaaa atagaaaacc aagaaggtgt agagaaccta
    10741 gatgaaatat taaaagtttc tgatggtata atggtggcta gaggtgactt aggtgttgag
    10801 ataccaactg aagagatgcc aatagttcaa aaaatgatga taaaaaaatg taatgaatta
    10861 gcaaagccag ttgttactgc cactcaaatg cttgattcta tgataagaaa tccaagacca
    10921 acaagagcag aagttacaga cgttgccaat gctatatatg atggaacaga tgcgataatg
    10981 ttatctggag aaactgctgc tggaaaatat cctgtagagg cagtaaaaat gatggctaca
    11041 atagcaaaaa gaacagaaga gactttagat tatgatagat tattaaagga aaatggtact
    11101 aacaatgtta ctgtaacaga tgcaataagc catgcaactt gtacaacagc agtagattta
    11161 aatgcatcag ctataataac atctacttct tcaggatata ctgctagaat ggtatctaaa
    11221 tttagaccaa aatctccaat aatagctact acaaataatg aaaagactat gaataaatta
    11281 gctcttacat ggggagtata tccagttaag tcttctgttg caggaaatac agatgaagtt
    11341 atagaaaagg caattgaggc agctagacaa gctgattata tagataatgg ggaattagta
    11401 gttataactg ctggagtacc agtaggagta agtggaacta ctaatttaat aaaggttcat
    11461 gtaataagcg aagaaatcgt gcaaggtata ggtgtaggta ctcaaacggt agaaggtaaa
    11521 gttcgtataa taaagaatgg aaatgcttgt gtagacttta atgaaggcga catacttgtt
    11581 tctactgcta ctgatgctga aatgaacaat tatatagaaa aagcaggagc tgtagtaact
    11641 gagaatggtg gaatgacatc acatacagct attgtaggta taaatttaga tataccagtt
    11701 attgtgtctg caactgatat aacaaatcta gtaaaagatg gagaagttgt aactgtggat
    11761 gcatcaagag gtgtggtata tagaggttca actagagttt tataagaaaa ataataaatt
    11821 tatttactaa ggggctttat aagctccttt ttttttggta aaataaaata ttgtagctta
    11881 gtaaaaaata tagtttaatt aatagcaata ttttaatata aatttaaaga aaaaagtttg
    11941 taaagattta aatataatag attgattgga gggtgattta tgctttcgaa aaacgagatg
    12001 tatattgtag atatagtaga cattggtcaa ggcggagtag gagtaggaaa gtacaatggt
    12061 attacagttt ttgtagaggg cggattaata caggataaag ttaaagttag gattaataaa
    12121 gtaaagaaaa attatgctgt aggagatatt gttgagataa tagaaaagtc tccattcagg
    12181 gtgaaaagag tgtgtagtga gaatcttagg gattgtggag ggtgtcagat acaagactta
    12241 gattataata aacaacttga aatgaaaaca aatgaagtta aacaagtact ttcgagaata
    12301 ggtaagttag aggatgtaga agttcatgac actataggaa tggataatcc atatagatat
    12361 agaaataaag cacaatttcc aatacaaaag aataatggta ttccaattat tggcttctat
    12421 aaaaagaaga ctcatgatgt aatatcgaca gaaaaatgcg taatacaaca tgagataaat
    12481 gataaaattg ttaaaataat aaaaacatat ataagggcct ataaggtaag tatatatgat
    12541 gaaaagactc acacggggtt gattaggcat ttagtaacaa aaataggttt tacaactaaa
    12601 gaagtaatgg ttgtattagt agcaaatgga aacaaacttc catatcttaa agaattagct
    12661 tcagtgctta aggaaaatat acctggattt aagactttgg ttgtaaatgt aaatcaaaaa
    12721 gatacgaatg ctattctagg aagagaaaat atagttatat atggagatgg aaaaataaac
    12781 gattatatag gtgacttagt gtttgagatt tcaccattat catttttcca agtaaaccct
    12841 gtccaaacta aagtattgta tgataaggct cttgaatatg caaatttaaa tgatactgat
    12901 actgtttttg atatttactg tgggataggt acaatatctt tatttttggc acaaaaggct
    12961 aaaaaggttt atggaataga aattatagat gatgccataa aagatgctaa aataaatgct
    13021 aaattaaaca atatacataa tgctgaattt tatgttggaa aggcagagga aattgtacct
    13081 aagatgtata gagagggtaa gactgccaat gtagtagttg tagacccacc tagaaaggga
    13141 tgtgacgaaa aggtattaga gactattgtt tcaatgaatc cggaaagaat tgtctatgtt
    13201 tcttgtaatc cttctacttt agcaagggat ttagactatc taaatgttca cggatttaaa
    13261 tgtgttgagg cacaacctgt agatatgttc cctcattcta cacatgtgga gacggtagca
    13321 ctattgtcca aacttgatgt cgataagcat attgatgttg aaattaagct ggatgagttt
    13381 gatttgacaa gtgcagagag taaagcaaca tacgctcaaa tcaaggagta tgtattagaa
    13441 aaatttgggc taaaagtttc tacactctat attgcacaaa ttaaaaagaa atgtgggatt
    13501 gaaatgagag agcattataa taaatctaaa aaagataatc aggctatccc acagtgtacg
    13561 cctgaaaaag aagaagctat catggatgct ttaagacatt ttaaaatgat ttagtataaa
    13621 tggagggtat ttatgagatg gatattaaaa ataatcttat ttccaattag cttgttgtta
    13681 agtattctca ctgcatttct ttcattttta cttggaatag gaacagcaat actttacttg
    13741 ctgatgatgt tttgtatatt cggagcaata gcatcttttc tacaaaaaga agttaccatc
    13801 ggcatagaag cattgattat tggattttta gttagcccat acggaatgcc gatggtggga
    13861 gcaactgtta tagcagtttt gcaaggagta aatgagagaa taaggttagt ttaaattaaa
    13921 gtattgataa atttgaattt gccgtagcaa ttataagtca tttggtaaaa aataaggtaa
    13981 taaaaatagg tgatggaatt gtactattct tggcgaatta atagatgtat caaaaaataa
    14041 aggagtaatc taaaatgagt tcatataatt ttaattatat aataccagat aattttaata
    14101 ggaggacttt tcaatattta caacagaaaa atagtaacct tgcaacaaca tttgctagat
    14161 gcacatatga gtatgaagat gttggattag cctattatgc tggattaaaa acaggggata
    14221 attggaacaa gcatgcttta gattttacta ttgagggtaa tcaaaatgat attgagatac
    14281 tgcaacaatt aaatagtgat ttaaaagatg ctattgataa agctttaaaa ccaagtgttt
    14341 cagggcttat aataagacat atttattatt taccttctga agaaagtaca ttagatttac
    14401 cacagtctaa tgtagctaga ttaaatgcgg atatttcaac agcagaagga gttttaaaag
    14461 atttaatcca ggtaagcgaa cgactatgtt caaattcatt atactcagat ataagtcttg
    14521 aaaatagtat aaacgattat tttcgtgatg cactttcttt tatggggtat aacgaggtta
    14581 aagatcaaac gcgacatgga gtttcaattt ctgaaaaaga cgctggagag gtcgatttgt
    14641 tacttacaaa aagtggtaaa gaaatagcta tttttgaggg gctaaaactg gatagtgtta
    14701 attcaaaata tattgatgaa catataaaga aagctataga taattataat gcgttaggta
    14761 ctgctacttt tgttgtggca tatgttaatg tagtaaacta tgatagattt tgggagaagt
    14821 actatgagca tatttccaat tatgcacata atatggaatg taaatcagcc atgaaagagt
    14881 taacacaccc taatgctgca actagaatag catatgaagt attatcgaag gatggatatg
    14941 attttccagt ttattttatg acaataaaaa tagcttagtc ttataaatgg taattagtcg
    15001 atgagctatt agtaagattc attcttgttt gcaaaataaa aaatttcata aaatcggtta
    15061 caaaaggcgg tagagaaaaa gctatcgtct ttttatttga ataaaaaaag aaaggagtgt
    15121 ttgatgagta tgtatttatt tgatgaacaa cctatagttg cgaataaaac tcttgctaga
    15181 gaaataggat taaacgaagc actcatacta cagcagatta attattggat agaaataaat
    15241 aaaaaatcag gaaagaatta tcatgatggt aaatactgga catataattc aattcgagca
    15301 tggcaagaaa atgattttga ctatatgagc tttgatacag taaaaagaac tttttcaaag
    15361 ttagaaaaag caggttatct tttggttggt aattataata aagatccaag agataaaaca
    15421 aaatggtata caattaataa tgataagctt gaagaattgt atgtggatat ggaattaaaa
    15481 aaacaaaaaa tacaaacaga ggcattaaaa aaatctatgc ccaatacatt agggcaaaat
    15541 gcaccaatgg ataagggcaa aatcaaccaa tgcaataatg cagactgcac taatgcacag
    15601 gttcaaaatg cactaatgca aagggataat ttgcaccaac cattaccaga gaatacaaca
    15661 aataatacta caaataattc agcagatatt tccatccatc cttccatcaa agaaaagaat
    15721 caattatttt cagagaatat gaagggaagg aggatagata taagctataa aaatatatat
    15781 caaaattgtt tagaagaatt gcgacaccaa acaggatatt atgaacatat gagcattggg
    15841 aataagattg tggctgaaga gtttgatgaa atattaaaga tactggcaga cgtaatggta
    15901 ttagatgatg aatctttcat tactatcaac caaacaaaga tatctgtaca agtagtaaag
    15961 gaaagattta gggaacttaa ttcagggcat ttagagtact tagtaggagt attaagagaa
    16021 aatgaataca agattagaaa tataagagct tttgtcctga ccgccgctta taatgcacca
    16081 actggaatag atgcctatta ttcggctctt gtaagctatg atatgagagg aggatattga
    16141 tgtgatcaat gaagaaatag caagaaagac cttaaatatg gaagttagag ctgccaaagt
    16201 aacaggaaag ttaattctgg acttattaaa gaaactgatg aaagaagcag aaaaacttgg
    16261 aggacttgaa aaactggtta atgttaacgg aaatgaagta aagctaaaag atatggttaa
    16321 aaagggacag cttgaagaaa tcccagtgga agaagctgaa cttaaagaac ttaaaaaaga
    16381 gttgaataga tacggtgtta aattttcagt gatgaaagat aaagagacag gtaaatactc
    16441 tgtattcttt caagccaaag atatgaaagt gatggataaa gcttttaagc acgctctttc
    16501 agaatcagaa aagaaagcag aaatgaaaga atctattcac aaaaatattg agaaatttaa
    16561 ggagatagct aaaaactcta tttcaaaaga taaaatcaaa aataaacaaa aggagcaaag
    16621 cctatgatag ataagatact aaaagagatc aaaggcttat ttaaggtgca agataaggca
    16681 aagtttctaa agcagaacat tccctatctt gcatttttct atgttggtaa tatcttctct
    16741 catcatgtaa gagcatatac tggtggcgat gtgatagaca aaatctttca aggaatacta
    16801 gagcttaata ttatgagttt tcttccaagt cttcatccaa ctgacattct aataggcata
    16861 ggagtagctg ctttaatcaa atttatcgta tataccaaag ggaaaaatgc gaaaaagttt
    16921 aggcagggta aagagtatgg ctcggcaaga tggggaacta gaaaagatat tgagccgtac
    16981 atggatgaaa agtttcaaaa caatatcctg cttacgcaaa cagaacgatt aaccatgaac
    17041 ggcagacctg ctaatccaaa gtatgcaaga aataaaaatg tgcttgtaat aggaggttca
    17101 ggaagcggaa aaactcgatt ttatgtaaaa ccgaacttaa tgcaaatgca cagtagctat
    17161 tgcgtaacag atccaaaagg aacgatagtc cttgagtgtg gtaagatgct tgaagataat
    17221 ggatatgaga taaaaattct aaataccata aacttcaaaa agtctatgaa gtataatcca
    17281 tttgcttatc ttagaagtga aaaagacata ctcaaattgg tgcaaacaat cattgcaaat
    17341 actaaaggag agggagaaaa agcgggtgag gatttttgga tcaaagccga aaaactctac
    17401 tatacagctc tgattggata tatcttctat gaagctccaa gggaagaaaa gaactttgca
    17461 acactccttg atatgataga tgcttcagaa gttagagaag atgatgaaac ctatatgaat
    17521 ccaatagata gactctttga agccttagaa aaaagagagc ctacgcactt tgcggtgaag
    17581 caatataaaa aatataaact tgctgccgga aaaacagcaa aatctattct catttcttgc
    17641 ggtgcaaggc ttgctccatt tgatattcaa gaattgagag atttgatgaa agaagatgaa
    17701 ctggaacttg atactcttgg agatagaaag gcagcactct ttgtcattat ctccgatacc
    17761 gatgatacct ttaactttgt agtgtctatt atgtattcgc agttatttaa tctcttatgt
    17821 gataaggcgg atgatgtata tggcggacga ttatctgtcc atgtgaggtg tctacttgat
    17881 gagttcgcta atatcggctt aattcctaag tttgagaagt taattgcaac aatccgttca
    17941 agagagattt cagcaagtat aatacttcaa gcacaatctc agttaaaagc aatctacaaa
    18001 gataatgcag ataccattgt aggtaattgt gatagcacct tgtttcttgg tgggaaggaa
    18061 aagactacat taaaagagct ttctgaaaca cttggaaagg aaacgataga cctttacaat
    18121 acttcggaaa caagaagcaa tcaaaagagc tttggattga attatcaaaa gacaggaaag
    18181 gagctaatga gccaagatga gataacagta atggacggtg gaaaatgtat atttcagctt
    18241 agaggtgttc gacctttttt atcagataaa tacgatatta caaagcataa gaattacaag
    18301 ctccttgagg actatgacaa gaggaatttg tttgatatag agagctacat taagcgtaaa
    18361 gggaaagtga aaatgaatag acatactgta attacaagat tgtaacattc tccttatcac
    18421 tatatgtaga tgtataaatt tggtataatg ttgtttaaat aaatgatatt ttatgtatga
    18481 aacctataat atttgtacta atactgtggg gtaggaaaag aaaacttcct atatactaaa
    18541 atcaatcgaa agaaaggagg aaattagaat gaatattatc aagtcattta acagtacaat
    18601 tgattatctt gaaacagttc ttgatgatga aattgatgaa aagaaagtaa ctagaatatc
    18661 tggatattct tattcgatgt tcagtcgctt gttttctatt ctaactgaaa tgacactttc
    18721 agaatattta agaagcagaa gattaacgga agctgcgatt atattaagag atacagatga
    18781 aaaaattatt gatgttgcat tcaaatttgg atatgagtca tcagattcgt ttgggactgc
    18841 ttttaagaat tttcatggat ttactccttc tgaggtaaga aatggaaaac cctttaaatt
    18901 agtttccagg gtgcaattag cattaagtgt aagaggagga agaagtatga atattaggat
    18961 tcaaaagaaa aaagcattta cagttgcagg tgtaaatcaa caaaacatca attcatcaat
    19021 atgtgcaagc ctatggcaaa aactattttc aaaggtatct catgaagaaa tgttaaactt
    19081 agggaccgga gaagctgtag gagtatgcca tgatatatta agtcttgata atcatgatgt
    19141 tttagatagc aacacgataa attatatggc aggatatatt gttacagatg tagataaggc
    19201 aagaagtatg ggattagata ttttagaaat tggagaaaca gagtatgcgg ttgtagagtt
    19261 aacaggtagt gttccagagt gtattcataa tggttggaag tatgcgatgg aagtattctt
    19321 tcccgagcat ggatatgttc attcaggaaa gccggacttt gaatattatt atgaaggaga
    19381 tatgtatagt aaagactaca aaatggaact ttggattcca gtatgtaaat cttaacaaat
    19441 ttcagtctat agttttaaaa caaaatatat aaaatacaag gtgatagaaa gtaaaagcta
    19501 ttgccttttt tatgtaaaaa tttaaggaga tagaatgata aggataagaa gtcctactta
    19561 aatgaaaata aaatagtaag agtataagcg attggcaaag tatgaagtcc ggtcgctttt
    19621 tttatttaaa agaaaaggag aaattttaat gaagcaagaa atgattaata ttaatgccaa
    19681 cttgttggca gaaccgactt tctcaaaatt tgaaaaagag ggagaaaaag tggaagttgc
    19741 aaacttcacg cttgtaaaaa agtacggaaa aggcaaggag tacattaact gtgcagctta
    19801 tggagaaaag gcagaaatag caaaagcctt tgaaaaaggt gatttaatcc atatctttgg
    19861 ctactttaag aagcgtgaaa aagagggaaa gacttacaag aactttgttg tgaagtcata
    19921 taacaagatt gaaaagaaag aaaaaaacga ggaggaataa atcatggaat tttttggaca
    19981 agcagtcaat gtattaaaga ttttagtaat ggcagtaggt gcaggacttg gagcgtgggg
    20041 tgttatcaat cttatggaag gatatggtaa tgacaatccc ggagcaaagt ctcaggggat
    20101 taagcagtta atggctggag gtggaattgt ccttattgga ttaaagctga ttccacttct
    20161 tgcaaatgta ctcagataag aagaaaagga gaaactaaat gtttggtata ttcgacaaaa
    20221 tagaggagtt tttcaaagag cttctattag gcggtatcca agcaaatctt gagtctatgt
    20281 ttcttgatat caacgataaa gttggtgcgg ttgcaacaga tgtagggaaa acgcctatgg
    20341 gatggaatgg acaagtattt agctttatta aaagtattaa cgattctgta attattccca
    20401 tagctggtct aatcattacg gcagtccttt gtatcgagct tatcaacatg gtaatgcaaa
    20461 agaacaatat gcacgatacg gatacctttg aatttttcaa gtacatcata aagatgtgga
    20521 ttgccgtatg gttagtatct catgcctttg agttttcaat ggcggtcttt gatgtggcac
    20581 aacatatggt aaataaggcg gcaggggtga taaacacctc tgccaccgtt tccggagatc
    20641 agatagtggc aatgatggat accttaaaag aaaagggact tggagaactt gtcatgattc
    20701 tctttgaaac ctctctcatc aaggttgcta tacaggtaat ctctgttgta atcatgcttg
    20761 tggtttacgg aagaatgttt gagatttatg tttactcatc ggtttctgca ataccgtttg
    20821 ccacaatggg aaacaaagaa tggggacaaa tcggaacaaa ttacatcaag ggactatttg
    20881 cacttggact acaaggactc tttttaatgg tttgtcttgg aatatacgca gtattagtta
    20941 agacaataca gataacagat atacacacaa gtaccatgac gatacttgga tatgcggttt
    21001 tgctggggtt aatgatgcta aagagcggaa cactggccaa aagcgtatta aatgcacact
    21061 aaaaaagaaa ggaaagatag tatgaacaaa agaaaaacag tatttacagc agtagtaatc
    21121 ggagcaggca ttatggcggt aattgacaga atcagatttc ataacaaagt ggacgaattg
    21181 gaagaaagaa cagaggacat cggtcgttgc cataatgatt tttgcttaat gcagcaaaga
    21241 tataacagaa gtacagatga acagattgta agtattcagg aagaaatcgg ctccgtatat
    21301 gagcatatag aagaactttc aaaggaaaaa gaagatggga ggtaagttat ggcatatgta
    21361 cctatcccta aagacttaaa gaaggtaaaa acaaaggtgg catttaacct gacgaaaaga
    21421 cagcttatcg gttttacgat tgcaggactt attggaatac caatctattt atttatgcga
    21481 aaggtagttc caaatgatat agcagtcata tttctcattg tgtccacact ccctatattt
    21541 ttcatcactc tttttgaaaa ggacggactg acatttgaaa aatattttaa atatatttac
    21601 cttcataagt tttatcagcc acaaaaaaga gtgagaaagg aggtttatct tgaaagacaa
    21661 aagaaagatt cagcagctaa agttagaaca aaacaaaaag aagttaagaa gtcaaagaca
    21721 ggacttaaag caaaataagg gaaaatctaa aaaagataaa ggcgggctac ttgaccttat
    21781 ctttaggaaa gaaccaaaaa gatatactgt tgaagatacc attccctatc ttagactctt
    21841 aaaaagcggg atatgtcaga ttgatgagaa gcattttaat aagagtatcg cttttgagga
    21901 tataaactat cagcttgcat tagaagaaga tagggatttg atttttaatc agtttgcaaa
    21961 ctttcttaac tcctttgatc caagtatcag catagagttt tcgtatatca atcagcttgg
    22021 cagaaatgaa gaaatgaaat cggcaattca aataccggat aaaggagatg gctttgacga
    22081 tatacgcctt gagtttcgag aaatgttgaa aagtcagatt gccaagggaa ataacggact
    22141 gaaaaaatca aaatatgtaa cttttacagt agaagcggat aatttagaac aggcaacatc
    22201 aaaactggaa agaatggaga tagatatatt atcaagcctt aaaagtatgg gagtaagagc
    22261 agaaagcctt aacggagagg aaaggctaaa gatacttcac gatgttttaa atccgaataa
    22321 gacctttgaa ttttcatata aggacttaaa gaaaaaggaa agtacaaaga catatattgc
    22381 tccagatgaa tttaacttta caccgtcaag gtattttaag tttggaaagt ttatcggagc
    22441 agcaagtcat tttcaaattc ttgcaagtga gctttcagac cgtatgcttg ccgagttttt
    22501 ggatatagat gataacatca atatctcttt tcatataaag gcaattgagc aatcagaagc
    22561 catcaagatg gtaaaaagaa aaaataccga tattgataag atgaagattg aggaaaacaa
    22621 aaaggctgta aggtcaggct atgatatgga cattcttcca agtgatttaa ttacctatgg
    22681 agaagatgta aaaagccttt taaaagattt gcagacaagg gacgagagaa tgtttgttgt
    22741 aaccattatc tttatgaact ttgcaaggac aattcaaaag ctggataata ccatttctca
    22801 aatcagctct attgcgaata aacataattg taagttaaaa agacttgacc actcacagga
    22861 acagggctta atcagtgtac ttccacttgg tgtaaataag attgaaattg acagaggact
    22921 aacctcatca tctacggcag tatttatgcc ttttaccaca gaggagcttt tcataaactc
    22981 ggtaaacagt ttgtactatg gactaaatgc cctaagtcat aacctgatca tggcagatag
    23041 aaagaaactg aaaaacccga acggtctaat ccttggaact ccgggaagtg gtaaatcctt
    23101 tagtgcaaaa agagaaatgg caaatgctat tcttacaacc gatgacgatg tgattatctg
    23161 tgatccggaa ggcgagtacg gaaaccttgt gcgtcagttt aaaggagaag ttataaaggt
    23221 cagcagtaag tcaaaggact atcttaatcc tttagatata aatatgaact atggagatgg
    23281 ggacgcacca ctgaaagata aggcgaactt cattatgtct atgcttgagc ttgtcgttgg
    23341 tggtagtggt ctaacagcag aagaaaagtc ggttatagat agatgccttc ctaaaattta
    23401 tgaaaagtat tttgaaaatc ccttacctga gaatatgcct attcttcaaa acctgtatga
    23461 tatgttaaaa ggacaggaag aaaaagtcgg aaaaaaactg gcaacggaaa tggagattta
    23521 tgtaacagga tctcttaatg tatttaacca tcagtccaat gtggacttaa ataagcagct
    23581 tctttgtttt gacattaagg agttaggctc gcagcttaaa aaaatcggaa tgcttgttat
    23641 tcaggatcag gtgtggaaca aggtatcgca aaacagagga aataaggcta caaggtacta
    23701 tatagacgag tttcacctat tgttaaaaga cgagcagaca gcttcctatt ccgtagagat
    23761 ttggaaaaga tttcgtaagt ggggaggaat tccgacaggc attacacaga atgtcaaaga
    23821 cttacttatg agtaaggaaa ttgaaaatat ctttgataat acggactttg tgctaatgct
    23881 taatcaggct tcaggtgata gagaaatttt agcaaggaaa cttaaaatct cacagccgca
    23941 gttaaaatat gtaaccaatt ccaatgcagg agaaggactt ttgttctttg gaaacaccat
    24001 tgttcctttc cttgataagt tcccgaaaga cacaatactt tatcagaaaa tgacaaccaa
    24061 gcctgaagaa gtgaggtagg cttatgggaa agaaactgaa aagggatttt aaggaaaggc
    24121 aaagagcaag aattgaaaag gaaacactgc aaagtagtgg cagcatagag tctgaagaaa
    24181 gtaagctaaa gcataatgat gattacagag ggaaaattgt ccatgacaaa gacagatttc
    24241 aagataaggt gcatgaaaaa acaagcaaaa taatttctga cagtgaaccg gttcgaggga
    24301 tttccaaaag aaatacaaga tatagaagtt ctgaaacagg aagtgtaagt cgggaagaaa
    24361 tcggaaaagc tgattatgct actgaggtta atgacggaat catagataga tatgacaatg
    24421 actttagagg cagcgaatat tttgagtcaa cctatgatgt agaggataat cttcatcata
    24481 aagccgaaga tacaaaaggc tttttaaaaa atcaaaaagc tcaaaagaaa aattataaga
    24541 gaaaaaacta ttccgataag ctctatacca gaaaaaagga cacagaggcc aaagcggaaa
    24601 aggcagacaa taaacaaggc ggaaaagaag ctatccttga tagagaaaag aaaaacaagc
    24661 tttctaaaga acaggaaaca attttcaaga ataaggcatc taaagtatct gctttatcag
    24721 gcttagcaaa aggaagtgaa accgtaagag attacttatc tcatggaagc gatgaaaatc
    24781 aaggggtaga ggcaggcgaa aagacagccg atacaagctc aaaactcatt catggcatta
    24841 agaactattc ggataagaaa agagcccaaa aaggatatga ccttataaat aaggactata
    24901 aaatcagaaa gcgaaaatcc aaattagagt ttcgagatgt caaagaggaa ctgaaaaaga
    24961 cggaggaata tcaaaaagca aataacttta agcgatttca aaagcgaaag cagatgaaag
    25021 attccattaa gaggcagaat aagtcaaggc ttagagatcg cattaaggaa gggcttatcg
    25081 gcagtcttaa gggttcaaag gaaattatat taagaaaaac gaaaggactt atgattattt
    25141 ttataggtct tattattttg ggaactttct tcattaactt tgcagggaca ggaatgacag
    25201 gttttatgaa ctctacaagt atagttctta caacaagtta tttatcaaag cccaatgttc
    25261 tcacagaaat taaccaacaa ttttctagta tggagcaagg attacaagat gagcttgaaa
    25321 gtgttgaaga aaatcatccc ggctatgatg agtatattgt taataaagag gaagatattg
    25381 gtcataatac ccatgaactt ttatcataca ttacttcgag atgtggagag gttaaaaaca
    25441 tatcagaagt gaatagaatt ttagatgagt tatttaagtc gatgtatcgt gtagattaca
    25501 aagaagaaat tgagataaga tataaaaccg tagctgaaac atatactgat gaggatggaa
    25561 acgaatatac cgaaagccat gaagaacctt acgagtacaa gaaactcatt gtaactcttt
    25621 ataaaagaga aatggatagt gttgttcgag aagtatttca aaattatcca gataatgtga
    25681 aacattatga agcattactt gcaagccttg gtaatatgag tgactttttt ggaagtggaa
    25741 gtaatgatta ttctgaaata atcaacaatc ctaattttgg caatccggga atagctttca
    25801 atgaagaaac agttaaacga cttttctcag aagcagaaag acatatagga aagaggtatg
    25861 tttttggtgc aaatgggccg tcaaattttg actgctcttc atttgtgtgt tggagtttta
    25921 ctcgttcagg tattaaaaat atgccaagaa caacggcttg gagaatttat acggattact
    25981 gtaatcctgt aagtacaggt gaagcaaagg caggagatat aatattcttt aaaaatacct
    26041 ataatagtgg aagccctatt tctcatgtag gtatatatgc cggaaatggg atgatgatac
    26101 atgcagggga tccaattcaa tatacttcta ttaactcacc atattggaga gaacattttt
    26161 atgggtttgg aagaccaaga taggaggata caatttgaaa aaagaactga cagcagtaaa
    26221 aaatagaata aagaaattga aagataaaaa ggctctgatt gatgaagaat tggagccttt
    26281 gttcattcgt gaagaagaac ttgaaaacga agaaattatt gccatttgca gaaagaataa
    26341 cattacaatt tatgatctta tggcaaaagt aaatagagaa aaatcagaaa tgaaaaagga
    26401 gaacggaaat gaaaaaaatg aagaataaaa aggctgcggg agtaattctt ggagcagtgc
    26461 ttttgatagt gggagtagta atctctttat ttgcaggtag taagattgta tttgcacagg
    26521 gagggttgtt tacgacactt gtaaatccga tagaagccgt tgctcaaata gaagtagaag
    26581 ttaatgttaa gtatattttt gaagatgaaa aagtctttaa ggaggaaaag attaaggctg
    26641 aaaaggggca gcttcttgac agtggagatc ttccaataat tcctgatgat atgaaattta
    26701 tagatgattt tctattttat gaaataaagg gagacgggaa agatgaaatt atccgtaaag
    26761 tagcaaaggt tgaggttaag gataaggaaa cgcaaacaga aaatgaaagc tcaaaatctg
    26821 agcaaagctc ccaatcaaat gcttcaaaga aagaaaataa gggaactcag acagaactga
    26881 gcaaagataa tatttccaaa atggaaaaag aatccaagga acttcaggaa aaacttgata
    26941 agttaaatca ggaaattaag gacaaggata aactaagcga taagcaaaag gacaaaatca
    27001 aagaccttga ggataaaata gataagctga agggaaaact tgaaaagagc aaagccgata
    27061 aggacttatc gacagatatg aaaaaagaga tggacaagct aactgataag attaaggaac
    27121 ttgagaaaaa agctaatgag gtcggtagag ctcctgttac gcataatcct actgtagcac
    27181 caatcaatcc tatttccggg attaaacaag gaacaggaaa tgtatcacca caagcgtcta
    27241 taaatagcag tggcaaaggt tctgcttcgc aggaaagtaa aggagcaaat acaaaaaata
    27301 tagatagtga taaatcaaag gaaaaagaaa aggaagttcg ttatccaaat aagttgacac
    27361 caaaacagcc tgctaataac ggtagtcagg attcttcatt ggatggttca agtaagaatg
    27421 taaataccaa taaggggatt gcttcagctc cttcaaaggc aagaggagcc gttacggaaa
    27481 ataaagataa tgcaaacaaa gattatccta ttcaccataa tgacagtaaa gataataaag
    27541 aaatggataa atattccgct gatgcaagac agtttgttac ttttacaact aaaaatggta
    27601 agacttttca tttaatcatt aaccatgatg agaatagcga gaatgtcatg cttctaactg
    27661 aagtatctga agatgatctc ttaaatatgg tagaaaagaa agcaccggag aaagaggttg
    27721 ttaaagaaga accacaaaaa gaagagctta aaccagttaa aaaagaagag agcagcaata
    27781 caggaatcta cttaattctc attcttgtag tagcaggagc attaggtgca ggatattatt
    27841 tcaaggtcat taagaagaaa gaagataagg agcttgaagg ctttgaggag gaagatgatg
    27901 atttcttttc agaggcagaa gaaagtgaaa atgaatcaga tgaaacagaa gataaagaag
    27961 atgatgaggt agagtaagtt tgcacctaaa actatacaat atttggtgca aaacacaggg
    28021 cgggagagta aaatctttcg cccttttata ttgaaaacag gaggaaaaaa agtatgaaac
    28081 ttgtaattgc tgaaaagcca agtgtagcaa tctctattgc aaaagtgata ggagcaacaa
    28141 agaagaaaga cggatattat gagggaaacg gatacaaggt aagctggtgt gtaggacact
    28201 tgattcagat ggcaaatcct gaaagctatg atgaaaagta cgccaagtgg aatatggagg
    28261 atttaccgat tatcccaaga gaatataagt atgagattgc aaagtccaca aagaagcaat
    28321 ttgcgattct taaaaagctg atgaacgata aggaaataga tattgtgata aatgcctgcg
    28381 acgcagggcg tgaaggagaa gccatcttca ggcttgtata taatcaggca aattgtaaaa
    28441 agaagatgaa acgcctttgg atttcctcta tggaagatag tgcaattaga gatggttttg
    28501 ataacctaaa agatggaagt ttttatgata atctctttga atcatcacaa gcaagggcga
    28561 ttgcggattg gattgttgga atgaatttaa gtaggttgta ttcgtgccta tataagcaaa
    28621 attacagtgt tggaagagtg cagacgccta ctcttgccat gattgtaaaa agagatgatg
    28681 agatagcaaa tttcaagaaa gaaaaatatt atacagtgga actttctatg aatggattta
    28741 cacttgcaac agacagaatt gatgatgaag tggtagctga gcagctctta aatttggtag
    28801 gagataagat agaaatcact gatgtcattc agaaagaaaa gataacaaaa cctgacttac
    28861 catttgacct tacaacactt caaagagagt gcaacaaata ttttggatat agtgcaaagc
    28921 agacacttga ttatgcacag agcctatacg agaaaaaact gattacctat ccaagaacag
    28981 atagcagatg attaacggaa gatatgatta caagtacaat caacaacatt attggaaagt
    29041 atgattttga tacagctcgt atcaagacag tatttaatac taaaaaggtt acagatcatc
    29101 atgcaattat accgacagca agcagtatga atgaggactt atcttcactt ccggaaagtg
    29161 aattaaaagt atatgaactt gtcttaaata agctgcatgc aagtgtaggt tatccgctaa
    29221 ttgagaatac aacaaaaatt atagccaaat ttgatggatt tgaatttaca agcagtggca
    29281 aggtgattgt tgatgaaggc tttaccaaaa acttaaaaga atatacaaaa aagaaaaatg
    29341 aagatacagt gcttccgtat gtaaagattg gtgatagtct tgatattaag gataaagaga
    29401 taaaagaaaa atgcacatca ccggctaaac attttactga agatcttcta ctaaaggcta
    29461 tggagcttgc aggaaatgac gcactggaaa aaggtgtaga agttgagcga aaaggacttg
    29521 gaactcctgc aacaagagca ggcataattg aaaatttaat tttcaaaggt tttattgaaa
    29581 gagataaaaa gaatcttatt gccacacata aaggaatcag tcttgtaacg attgtagaag
    29641 atacttttaa atcagcagaa acaaccgcaa agtgggaaat ggagttatcg gatattgcac
    29701 agggaaaatc ttcaaaggaa aattttttag aagcgattga aaatgagata aaaaaagtgg
    29761 tttcaactta tagcaagtaa gatgatacta atttcaaagt ggaaaaaaag ccctaaaaat
    29821 gctataatat tttgtaggaa aagtaggaaa tatggaggtg ctttatgtcg gaagcattta
    29881 tgaataatgc aaaggttatg tcaaaaggac aagttactat tccaaagaaa atcagagaaa
    29941 ttttagaaat tgagaatgga gattatgtaa cttttgttgt aactgaaggg aaaatacaaa
    30001 ttgttaattc aaaaactttt atagagaaaa atatacaggg caggaaataa attatatatg
    30061 tcagatgcag ataggaggaa tttatgaata aagaaaagaa ttctttgcaa aatataaaca
    30121 tagaaaaaaa caacaattca tttgaagaaa ttgttatgat tgtagaaaat gcaaaggatc
    30181 gtgcatatag aagagttaat gaagagctaa tattaatgta tcaagaggtt gggaaatata
    30241 ttagtgaaaa aacaaaggaa gcatcttatg gttcaggatt cgtggataat gtagctgaat
    30301 ttttttctat taactatcct gatttaaaag gatttaatcg cagagggctt tacagaatga
    30361 agcaatttta cgaactgtac aaagatgacg gaaaagtgtc aacgctgttg acacaattaa
    30421 gttggtctaa tcatttaaaa ataatgtcag gagctaaaag tagagaagag agagaatttt
    30481 atattaactt agcaattaag gaaaatttga ctcatcgaga gcttgtaaga cagatggata
    30541 gtggatacta tgaacgctgt atgctttcaa atgagggaaa tatgcctgca atacaaagag
    30601 cgaaacagga aacacataat ttgtttatgg atagctatgt attagaattt cttgatgctc
    30661 ctaaaattgg aaatgaaaga gactttcaga agtcaattct tgaaaatttg aagaattttg
    30721 ttttagaaat tggaaaagat ttttctttta taggtaatga gtatagagtg caggttggaa
    30781 atcatgacta ctatattgat ttattatttt accatagagg tttatcctgc ttggttgctt
    30841 ttgaacttaa aatcggagaa tttaagccgg aatatgttgg taaaatgaac ctatatctag
    30901 aagctttaga tagagaagtt aagaaacaag cggaaaaccc aagtgttgga gttattcttt
    30961 gtgcatcgaa agacgatgaa gtagttgagt ttgcattaag cagaagcctt tcgccaacaa
    31021 tggtatctga atatagatta aaattgatag ataaagaact attacaaaga aagttaaaag
    31081 agtatacagg aattgcagag caggagatag aaagatatga tgaaaaatag ataaatataa
    31141 ctttgtgctc aaaaaatgag tgaagtagtc catgtggaaa caataataaa ataaaagtgt
    31201 ttcaatttag taatatacta tgaaaggaga tgaaaagaaa tgcgtacagg taatgaattt
    31261 aacggtgttg aggatacttt atatatacct ttagctgcca gaatatatgt atctgaaaaa
    31321 ttcccacatt ttttctatga tgaaaaggct ctatctttga aatcgcagat tccaacaggc
    31381 ggtatagata acaatagtac ggaatatttt tatatggcta gtgtttgtag acaacagacc
    31441 atagatatga aaattaaaaa attccttgat gaaaataaaa agggaaatgt ggtattctta
    31501 ggatcaggtt tagagacttc atataatcgc ataggaaatc aaattgtaaa tttctatcaa
    31561 gtagatcttc cggatgtaat agaagtgcga aaaagagtgc ttggaaatgc taaaaatgag
    31621 caacttatat caggagatat gtttgacctt aactggatta aataaataga tacttcattg
    31681 ccgacactca tttctgtagc aggtgtatat cagtatttta acgaatataa aattatagat
    31741 atgattaaaa aaatgaaagc tcaatttcct aatggagaat tgatatttga cgcaacaaac
    31801 tctaaaggtt tggagcttgc taataaatat gtgaagaaaa cgggaaataa aaatgcacaa
    31861 atgtatttta gtgttgataa tcctaaagat ttagcagata agacaaaaac aatactattg
    31921 gaagttgatg gattttttca tggagcattg aaggattgtc agggattaaa actaatgacg
    31981 aggatatata tgtattttgc agataagttg catcgaacag tggttataca tttgaagttt
    32041 aattaaattc tattcaattg gtttttatta cttgtattca acgtataata tatagaatca
    32101 aaaggtgatt agagtaaaat ctaaccgcct tttttgtttg taaaaaagaa ggaggaaata
    32161 tgcgaataaa tgattttcat aacgttttgg agcttgtaaa acaggatatt ctgcaaagtg
    32221 aagcagaata cctgaagctc ttaaaggttg tcggaaacaa tcagagatac gactttagaa
    32281 gtcagttaag tatctatgat agaaatcctg aagcgacagc ctgtgccaag tttgattatt
    32341 ggagggagcg atttaaccgt actgttgtac gagggcaaaa gggtatcccc attttagagg
    32401 actacggcac atataaaaaa gtggactata tctttgatat agagtagaca gtttcaagaa
    32461 acagagatgt caatgaagta aatctttgga gatttgataa agaagtccat agagatgtct
    32521 taaaggaaat gataaaagct gagggatatg aagaaagcga aagtacctta gaaaatatct
    32581 tttctttaag cagactttat ggcgatgaaa agatagacag tcttatgaat gagcttagaa
    32641 tagctgatga agatagaata tcctttacga agtttgtgag ggattcagta agttatgcgg
    32701 tagcttcaag gtttaagtta gattatccga tgaataataa gcttctaaag gaaagcttta
    32761 caatgcttga tagtatttcc cttatgagct taggtgaaac tgtatcggat attagcggaa
    32821 agattattga tgcaatcatt caaaaaagca aaaaactgga gcttcaaaaa gaagttttaa
    32881 gaggaaaaga agcagaatat aataaaataa aagaagaatt agaggaggta gaagaaaatg
    32941 tacttcgacg agatgatcag gaaagaatta gtgaaaacga gcgaattttc cgaaatgaag
    33001 agtacggacg agataataga gaaaatcaag gagaatacgc tgaatcagtt agaggaagag
    33061 atggacttta tgagggaata tccgaatccc acatacgcag tgatgaggct ggagtttctt
    33121 tcaaacagcg aggagcagag tcacttcgag atgttattgg atctatacaa ggagaagaag
    33181 ctgaccaaat acctaatgga tattcagaaa caggcgatag agtttatgag agcagagaag
    33241 ccgaaactga tgatggcttg gaagatagag gacgagaacc atccgcagta caaagcgatg
    33301 atttcagccc tcaaagaaat gacaatcagg gaagtagtgg aaatctaaaa gagaatacgg
    33361 ataaagagat aagagaagct gacagggctt ctttttcttt acctgaaaac agttcaggac
    33421 aaattaaatt tacgatacct cttacacaaa atgatattga tagtattctt gtaaacggtg
    33481 gaaaccacga gggtggcaga cttcctatca ttgctgaatt ttccaaagga aaaacagtag
    33541 acgaattagg agaatacctt aaagatacct tcaaaggcgg aaacggttta cttcttaatg
    33601 aagcagaagt atcttcctgg tattcggata aaggcattca tttagcttat ggaacttcag
    33661 caagagaaga tatgacgcaa attttaagct ggaatgatgc tgcaagaaga ataaatgagc
    33721 ttttgggaaa tggagagttt gccacaaatg tagagctatt agaagcacag gactatgaaa
    33781 gagatagagt ttcagagtcg ctatggtatc tatatcacga tttaagcgaa gaaggaagag
    33841 cacagggcta ttttgacttt atagaaagag gtggtggctt tccgaaagaa acaagacagt
    33901 tatcggaagc tcttaaaaat cccgactatc tgaaagatgt tatttctgaa tacagtcgct
    33961 ttttgaaagg atataaagaa aacaaagatg tactaaggtt tcactatcac aaagtagaca
    34021 gcctttataa gaacttacag gagcttgcac ttccgagaaa ggaatacact tctaacctta
    34081 ctgaacttcc acaggtacat ggctttatta cagaagatga agtccttgct acgatttcaa
    34141 gaggaagcgg cttcgatcaa ggaaaagaga ggattacaaa atattttaaa gaaagccaca
    34201 ctttgcagga aaaagcgaat ttcctaaaag atgagtatgg aatcggaggt cattctcatg
    34261 ctgtttcagg ggcaaaggga agtgatgaat ggcacgatgc aaaggggctt aaattacaga
    34321 agaatcattg taacgatgtt tttcttacat ggtcaaatgt agcaaagcat atagatgagc
    34381 ttatttctaa aaatctttat cttaaagaaa ataaaattga aagtaaggca gaaatagaag
    34441 aacaaaaaga gccaagttat tactctaaag acgatccata taatctaatg actgatgaaa
    34501 tgcttgagcg agtgcctgag ctttatgcac aagaggatgt agctttagcg gataaacagg
    34561 ttcatgcagc atacattatt ccatttagaa gtaactggac ttggtatatg acagagtacg
    34621 atagagaaag taaagatgct tttgggcttg ttttaggtat tgaaccggaa tggggatatt
    34681 ttaatcttga ggagctaaag gaactaaatg cccagaggct tgttttagaa gattttccaa
    34741 agacctttag agaacttaaa aacagtgaac ttaaaaagca gatggatgag caggagcttc
    34801 atcaagtatt taatggagag ctaagttttg aggagaataa tacagagctt gaattacctg
    34861 aagaagtaga agaaagcata caagcagaat cttttcaggc taccttattt gattatctaa
    34921 aggaaagaga agaagtagag cttaatgaaa aagaggacag cctttcagat gagtttgcgg
    34981 ttaaagaagg cgatactgtc tattttaatc atgaggtgta caaagtaaga gagattttta
    35041 aaaatcaaat cactggaaga aatgatttgt ggcttgatcc tgtaagacaa ggtaatcatc
    35101 aaataccgat tgtaggcttt acagataatg aggatttatt aagacaaata agtcttgaaa
    35161 gacctgaatt tatcatcggt gatgaagtga agtacaaggg aaaagactat accattacaa
    35221 gatttgatga tatgggaaat aacctaaaga cagtaacagt caaggatgat accgaatatc
    35281 ttggtggtat gattacaggc tccgatgtaa ttccttatcg ccaagaaagt gagcttgata
    35341 aaatctttga aaatctgacc tatgaaaaag atgagcttta caacattgca gtaagaatag
    35401 atgagatagc ctatgatttt gatccttatg actatgcgga ttccgtagga acagaatcag
    35461 aggaaaggct gaataatatc aaccaaatct atggtgattt acaagcggga aatatttcag
    35521 cgtatataag tggtctgaat gagatgattg aagaagatgt tgaagatgcg gaaagtatca
    35581 agcaagtctt aactaaattg gaagaaaaaa agattgatat tcagcctaaa caagaagtat
    35641 taaacttcaa gattacagaa gaaacgcttc ctcaaaggtt atctccaagt gagaggttaa
    35701 ataataacct tgaagcaata tctatgctta acagagtgga aagcggagaa cgagagcttg
    35761 atattacagc tcaggaagtt ttagcgaaat atgtaggctg gggaggactt tccgatgtct
    35821 ttgatgaaag taaggatgga cagtggaaag aggcaagagc tttcttaaag gaaaatctat
    35881 cgtcatcaga atatgaagct gcaagagaat caaccctaac gagcttttac acaccaaaaa
    35941 ctgtcataga cggaatatat aagacgcttt ccagtatggg atttaaacag ggaaatatcc
    36001 ttgaaccatc aatgggtatt gggaacttta taggcaatat acctgatgaa atgagtaagt
    36061 caaagtttta tggcatagag cttgattcgg taagcggtcg tattggaaaa ctgctgtatc
    36121 cggaaagtga agtacaggta aagggactgg aagaaacggg attctccaat aatttctttg
    36181 atgttgcaat cggaaatgtt ccatttggag agtacaaggt aaatgacaga gaatataacc
    36241 gcaataactt tcttatccac gattatttct ttgccaagtc catagataaa gtaagaaacg
    36301 gcggtgttat cgcctttatt acatcttcag gaacaatgga taaaaaagac gaaagtgtaa
    36361 gaaggtatct tgcagcaaga gctgaatttt taggggcaat cagacttcca aacgatacct
    36421 ttaagggtgt tgccggcaca gaagtaactt cggatattat cttcctaaag aaaagagata
    36481 gcgtattgga gcgagatgaa gattggatac accttgcgga agatgaaaac gggcttatat
    36541 ataacaaata ctttgtcgat cattcggagc aggtacttgg ttctatgcgt gaagtaagcg
    36601 gaagatttgg gaaaactcta acttgtgaac cgatagctta tttagggcaa gagaataaca
    36661 tggcttcatt aaaggatcgt atcgagattg caggagaaag aatttcaaaa gatgccaagt
    36721 atgaagaaat agagctttta gatgatgaaa tagcctctat ccctgcaacc gatgatgtaa
    36781 agaacttctc gtatacctta attgatgatg aagtctatta cagagaaaac tcactcttta
    36841 tcaaaaagga agtatcagac aagaataaag aaaaaatcaa ggattaccta aaactgaatg
    36901 ctgtcttaaa agatgtgatt tacaagcaga aggaagattt tagtgaggaa gaaattaagg
    36961 cttcacagga aaaactaaat gaagtatatg acaacttttc aaagaagcat ggttttgtaa
    37021 ataatttaag taataccaga gcattgaaag aggatagtaa ctttccgctt gtttcctcca
    37081 tagaaatcct tgatgaagaa gaaaatttca aggcaaaggg agatattttc tcaaaaagaa
    37141 ccattacaaa agcgaaagtc atagaccatg tggatacttc tttagaagct cttgttttat
    37201 cggtatctga aaaaggttat gtggactttg actatatggg aagtcttata ggaaaggaca
    37261 gaccgacttt aattgaggag cttcgagggg aaatctacct aaatattaga gaagagcaaa
    37321 acttttatag accactttcc tttaacttgg aggatggcga tttacctttt tcctgtgcaa
    37381 atggcagcaa ttcctataaa tatggctatg taacaaagga tgaataccta agtgggaata
    37441 tcagggataa gattgctata gtcgatagtt atctatcaaa attaagacaa acggaaagag
    37501 aactacctca tcttggctat gaagaagatg gaaaagaaaa agagctgata agctatgaaa
    37561 tgaaccgttt agaatatcaa aaagcagagc ttatcaaagt tcttccaaag gaattagaag
    37621 caagtgaaat caatgtaagg cttggtgcaa cttggatacc gattaaagat attgagaaat
    37681 ttctctttga aactctaaaa actccggggt atgcaagatg ggatattaag gttaaatttt
    37741 caaaccttac aagcgagtgg aatatagaag gaaaaagcaa agatagagga aatgaccttg
    37801 ctgaaatgac ctatggtaca agtagagtca gtgcctataa gctcattgaa gatgctttaa
    37861 accttaaaga aacaaagata tttgaccaga ttgtaaatcc ggatggttca aagacttcag
    37921 ttttaaataa aaaagaaacc atgcttgcag gacaaaagca agagctatta aaagaagaat
    37981 ttaaaaactg gatatttaac gatcaggaaa gaagaaaccg ccttgtaaaa ctgtataatg
    38041 agcgttttaa ctccatccga aacagagaat atgatggaag taacctttca tttgagggaa
    38101 tgaatacaaa gattgattta agacctcatc aaagaaatgc tatagcaaga agcctttatg
    38161 gaggaaatac attgcttgcc catgtagtag gcagtggaaa gacctttgag atggtcgctt
    38221 ctgcaatgga aagtaaaagg cttggaatgt gcagtaagtc cctgtttgtt gtacccaatc
    38281 acttaacagg gcaaatcggt cgtgagttta tgcagctata tccgtcggct aatattatag
    38341 tagctgataa aaaagacttt gaaccgaaaa gcagaaagcg atttatcggt aagattgcaa
    38401 cgggggaata cgatgcggtt gttatcggac atacgcagtt tgaaaaaatc cctatgagta
    38461 aggaatatca ggagaagcac atccaagatc agattgatga aatcgtaaac tatgtagaag
    38521 aatacaagca tgatagaaac caaaacttta cggtaaaaca gcttgaaaag acaaagaaaa
    38581 aactagaaac aaggcttgag aaattaaatg atgattttaa gaaagacgat gtgattacct
    38641 ttgaggaatt aggagttgat aagctctttg ttgacgaggc acataattac aagaaccttt
    38701 atctctatac caaaatgaga aatgttgcag gtatcggaca gtcagaagcc tttaagtctt
    38761 ctgatatgtt tatgaagtgc cgttacatgg atgaaatgac aggaggaaaa ggcattgtat
    38821 ttgccacagg aacgcctgta agtaactcta tgacggagct ttataccatg caaaggtatc
    38881 ttcagtatga gagcctaaag aaaaatcact tggagcattt tgattcttgg gcttccactt
    38941 ttggagaaac acaaagctcc tttgaattat cgcctgaagg tacaggatat agggtgaaaa
    39001 caagattttc aaagttctat aacttgcctg agcttatgag tatgtttaag gaagttgctg
    39061 atattcagac tgcggatatg cttaaccttc ctacacctga agctaactat gaagttatta
    39121 aaacggagcc ttctgaagaa caaaaggaaa ttctaaagag cctttctgaa agagcagatg
    39181 atgtcagaaa tagagttgta gagcctgatg aagataatat gcttaaaatt accaatgacg
    39241 gtaagaaact tgccttagat cagcgtttga taaatcccct acttcctgat aatcccgaaa
    39301 gtaaagtaaa tgtgtgcgtg aaaaatgtat ttgctatttg ggataagaca aaagagagta
    39361 gatcaacaca gctacttttt tctgatatgt ctactccaaa aggagatgga gaatttaaca
    39421 tctatgatga cattagagaa aaacttgtag caatgggcat accgaaagaa gaaattgcct
    39481 ttattcacga agctaattcc gataagcaaa aagatgaact ctttgcaaag gtaagaaaag
    39541 gcgaagttcg tatcttaatg ggttcaactc aaaagatggg agccggaacg aatgtgcaaa
    39601 ataagctgat tgcacttcat gatttggatg taccttggcg tcctgccgat ttggagcaac
    39661 gctctggaag aattgttcgt caaggtaatg aaaacaatga agtaaatatc tatcgttata
    39721 ttacagagaa tacctttgat gcctacctct ggcagacgat agagaacaag caaaagttca
    39781 tttctcagat tatgacaagt aagacgcctg ttcgtgtggc agaagatgta gacgagagct
    39841 cgcttaatta tgctgagatg aaagcactgg ctactggcga tccgaaaatt aaggaaaaga
    39901 tggacttgga taatgaagtt accaaactta aaatgcttga agcaaactat aagtccaatg
    39961 gctatagatt agaggataag atagctaaaa cctatcctga agaaatagcc aggactgaaa
    40021 agctaataga agcagttaag aaagatataa ccaatgttga accacaagga actggagaaa
    40081 ataagtttat ttcgatcact attggtggtg aaaaaatctt agataaaaag cttgctggcg
    40141 aaaaacttct tgaagctatt aagacagtta aaatcaatga aagtaaggtg attggtcagt
    40201 atcgaggtat ggatttagaa gtgagttata acttctttac aaatgagcat aactttagct
    40261 taaatggtgc tacaaagcat acaggtaaac tcggtacaag tgcagatggt aattttacga
    40321 gacttgataa tgcaatcgag aatatgccag aaaaactaaa tagacttgaa gaaaagctta
    40381 tagctactaa agagcaattt gaaaacgcca aagaggaatt aaaaaagcct tttgaaaagg
    40441 cagacgagct aaagactaaa gtgcttagat tagcagaatt aaataagctt cttgatatgg
    40501 gagaagtaga cgaaaaagaa aatcctaatc cacttcttga agatgtaaaa cgagctattg
    40561 tagatttctg taagagtgaa tatgaggaca atagttatac ctatgaaaac ttcaataatc
    40621 tatttcctga ccttgctcat attggaattg cctatacaac tacaccagat gaaaagcatg
    40681 agatacagta tgaaattagc ttagaagatt tcacatctac tcaatatatc aatggtgagc
    40741 ctattaccaa gattgactat ctaaaagacc ttggaagtga agaaaaagca ttagagtttt
    40801 taaaacaaga aatggaatat ggagactttg gcgagtttgt atccattaat gataatgacc
    40861 ttaaaaaagc tctaggactt gaaagagacg attatggtaa tatctatgat ccacttgcaa
    40921 aagacttgga taatgatggt gtaccagata gatatgataa tgactttaga gatagtgatt
    40981 actttgaaac aacctatgat gtagaagata atcttcatgt aaaatctgaa agcacagaaa
    41041 aggtatcgga gaaaccatca atcttaagac agattaagtc atatcagtct gaagaaaaac
    41101 aaactgaagc aagagaaaat aaatcaaaag aacatgatga acgttaaggg gcttatatca
    41161 agctcctttt cgctttttag tagaaaatat ggaggtgtat gatgaactac aaagaaatca
    41221 gaaacacatt agaacaaatg gcaaatgaaa gccatgaaga ctttgcaaag gcacttataa
    41281 gctttgaaaa aggtataaat gacaaggaag cattggataa gctatatcaa gagtatatgg
    41341 aaaatgatag tatgagccta cttaatgatg agtttgatta cttgattgag gagcttagag
    41401 aaaatggaca gattaaagat actgcagcaa tcgaaaaaga agataatgac cttgtaaaca
    41461 ttgttggaaa tatcgtaggt gaagtagatg ttgtagaaag agaaaataag aatggcgaag
    41521 tctttaaagt agttaatttc tctgttgcat ctaaagatga tgatggaagc aagacgtaca
    41581 caaactgctc ggcttatgga gataaagtag aaattccaaa ggactttaaa caaggcgatt
    41641 tcgtaaaact atttggtcag cttagaactt ctattgatga taatggcaag gaatatagca
    41701 atgttagaat cctatcttca aagctcttaa aagcaaaaga acagatgaag aaccaggaag
    41761 aaaagaaaga atctgttctt ggagcgatta agaaatataa ggctgaagag aaggctaagc
    41821 caacagagaa aaaagaagct ttaaaagaaa ctgaaagata agatgtgtcg gtgtggtctt
    41881 cggactgcac cgattatttt ttttgtcttt ttgtgtatag caacagtttg ttatttgtga
    41941 tataatttat gtataaaaaa tccaagttta tggaataagg agaaataata atgaaattta
    42001 actacaatgg tctttggaaa gtattgatag ataagaatat gaagaaaaaa gatttaattg
    42061 atagaacagg tatatctcca acgacaatat ctaagatggt tagaggagaa gcagtctcgt
    42121 tatctgtaat aggaaaaatt tgtgaagagc ttggaacaga tattggagat ttaattcata
    42181 ttgaaaaagg tatcgaggag gataaataaa tgcaaagtac atataatgca cttgaatata
    42241 ataagttaat tagttttatt tggtctgtag cagatgattg ccttagagat gtttatgtaa
    42301 gaggaaagta tagagatgta atccttccga tgactgtaat aagacgattc gattctatca
    42361 ttgagccaga aaaagcaaat ataatgaaag ttaaggaaat ggcggagaaa caaggctggg
    42421 atgttgcaaa aacattagat acagcagtgg ggttgccatt ttataataca tctaattttt
    42481 gcttaaaaga cttgaagcat gagactaaca gacagaattt gaaaaagaat ttcgaagaat
    42541 atttaaatgg cttttcagag aatgtaaaag agattcttca aaagtttgat tttaataacc
    42601 agctaactaa gatgactgat gctggtattt tgggctctgt aatcgaaaaa tttacatcaa
    42661 gtgaattgaa tttaagtcct tatgatgaga aaaactcgaa cggtgaaata atcaaaaaag
    42721 gcttagataa tcatgctatg ggaacactgt ttgaagagat tattagaaaa tttaatgaag
    42781 aaaataatga agaagcggga gaacacttca cgcctcgtga tgtaattgag ttgatggcgg
    42841 atattactat gtatcctata atggataaga ttaaggatgg gacttactca atctatgatg
    42901 gtgcatgtgg tactcttggt atgggaactg tagcagagga aagattgaaa gacttcgcaa
    42961 aagaaaacgg aaaagaagta tctatccatt taattggaca agaagtcaat cctgaaacat
    43021 acgcaatttc taaggcggat ttgcttatta aaggtggaga tacagactca aataatgttc
    43081 attatggttc aactctatct gaagacaaga catctggtca gcattttgat tttatgctat
    43141 ccaatcctcc atatggcaaa tcgtggaaaa tggatttagc aattttggga attggagaag
    43201 acaaggattt aaagaaaaat ataattgata agcgatttgt tagaaattat aaggaacaaa
    43261 atgattttag aatgattcct gatgtaagtg atgggcaatt gcttttctta ttgaacaata
    43321 tttcgaagat gaaagaaact gaattaggct cacgcattgt tgaagttcat aatggttcgg
    43381 ctctttttac tggtgatgca ggaaatggtg caagtaatgc tagaagattt atgattgaag
    43441 aagatctcat agaagctatt atccagttac cagaaaatat gttttataac acaggaataa
    43501 ctacttatat atgggtgttg tctaatagga aagaagaaag aagaaaaggc aaaatccaat
    43561 taattaatgc taatggtata aaaacatcat taagaaaaaa tatgggaaag aaaaactgtg
    43621 agttctctga ggctgataga agattcattc taaatgaata tctaaagttt gaagaaaatg
    43681 agtattccaa aatattttct aatgaggaat ttggatattt taaagttact gttgaacgtc
    43741 cattaagaca agcagtttta tgcaattatg aaaatataaa tgaagtagag aaagaacttg
    43801 aaaaaattgg agcaactaca ggaaaaatag ataaaaagct gattgctgaa agttttgtaa
    43861 aaggaactgc tggttctatc aaagagcttg aaaagaaaga aaatataaaa gcatatttag
    43921 aagtacttag agaaatggaa agttctgaaa agtatttaga ttatgaggct tttgaaaaag
    43981 cttttaataa gaatttgaaa aacaaaaata taaaaggtgc tagttttagt aaacttgtat
    44041 ctacgggact attagcaaat atgattatta gagatgagga agctgaagtg caaactgatt
    44101 ccaaaggtaa tttaattgta gatcctgaat taagagacac ggaaagcatc cctatgacat
    44161 ttgtaggtgg aattgatgag tttataaggc aagaggtctt accttatcat gaagatgcct
    44221 ttgttgatga aagtaagacg cagataggat atgaaattaa ctttactaag tacttttaca
    44281 aagccaagaa acttgaaaat gtggaggata ttgtttgtcg cattaaggaa ttagaaaaaa
    44341 ggtcagacgg aatgatggaa actgtattgg agggtctcta tgaataagta tgagagatat
    44401 agagatactg ggctaatatg gataaataaa gttcctaaaa aatggaatct acaaaaaatt
    44461 aatgcagttt ttgatgagag aagagaaaaa gtatcagata aagattatga agcactttct
    44521 gttacaaaaa atggaatatt taagcaacta gataatgtag ctaaaacaat agacggtgac
    44581 aatagaaaaa aagtgaaaat taatgatttt gttataaata gcaggtctga taggaaagga
    44641 tcatcaggat taagcagatt tgatgggtct gtttctttaa taaatactgt tttaaagata
    44701 aagaaagagt atccacgata tatgcactat ttgttgaaat ctgttccgtt ccaagaagaa
    44761 ttttatagaa atggaaaggg tattgtagca gatttatgga gtacaaactt tcagtcaatg
    44821 aaaagtatta ttttaccaat acctccaatt gaagaacaag ttcaaattgc aaactatctt
    44881 gattggaaaa tcaatgagat aaataggtta attcaaatag aaaaggagaa gataaaagag
    44941 ttagaaacat taagatttaa tgctatttcc gaatttgtat taagaggaat tggtacgcag
    45001 aattataaaa agagtagtat aaactggcta gatgaaatcc caagtcattg gaatgaggta
    45061 tcaattagat ggtgtgtaaa tattattaga ggtaattcaa cctttacaaa agatgatttg
    45121 cagaatagag gaaaatatgt tggattacaa tatggaaaag tttataaaac cgagataata
    45181 gattcagagt ttgattttta tgttagtgat aaattttata aacctgctca agttgtaaat
    45241 aggaatgaca ttatcatagt gtccacttca gagacagtgg aagacttagg acatacatca
    45301 ttttacgata gagatgatat tggacttatt ggtggagaac aaatattatt aaaaccatca
    45361 aacaatatta attctaaata tctcttctat ttatctaaaa ttttccgaat gcagttgcaa
    45421 ttatgtgcta caggaataaa ggtttacaga tttaaaatta gcgatttgaa acaaatatat
    45481 gtacctttgc caccaatgaa agaacaagaa aaaatagttt caaatataga attaaagctg
    45541 gaacaaatag atgaaagagt gaagaataat tatgctttta ttaaggagtt agagttactt
    45601 aaacagtcac taatatcaga agttgtaaca ggtaaaattg atgttagaaa tattgatatt
    45661 tctgaatatg aaaaagtaac tattatagag gatgaaatag aagaatttga tgaagtaggt
    45721 ggtgaagaag atgggaatta gagatacaaa gatggaagcc gaacttgaaa ataatatcat
    45781 tgagtatctt acgagagagc aaaagtatgg tgcttatcag tatattactc cagatgaaat
    45841 gaaactttca tataatcgaa agtatgcctt tgatgaaata cgattactaa cattcataaa
    45901 ggaatcacaa ccaggagaat tcgatatact tagattagat acagaagacg gaaaggataa
    45961 gttttttaaa cagcttgatg tatctataaa gcaaaaggga attgtggctg ttttaaaaaa
    46021 tggaataaaa tgttatccgt cttccggaac aattatcttt tatcacgctt tggatcccaa
    46081 aagaccgagt tcgtatgatg aatttaggga gaatattttt acagttacga atcaactgac
    46141 atattctgat aaaaatagag gtttagagct tgatttagct atttttgtaa atggtctgcc
    46201 tattgtaaca atggaactta agagtagagc ttccagtaat ggttggacat ataaagatgc
    46261 tgaggaacaa tataagaatg atagagatcc aaaggaaacg atatttagct ttaagaggtg
    46321 cattgcacat cttgccgtag atgaaaattt tatctcattt acaacaagat tagatggtaa
    46381 aaatacaaga tttatgccat ttaataaagg caccattgat ggtggctctg gcaatcctat
    46441 taatccagat ggaacaatga cggattattt atggaaaaac tttttagaca aagaaatttt
    46501 aacaagctta attagggatt ttgcttatat ttcagtagat aaagttaaga aaagcgagac
    46561 attaattttc cctagatatc atcaatatag ggttgtaact aggcttgtag atgatgtaca
    46621 gaaaaatgga gttggaaata gatacctaat tcagcatagt gcaggtagtg gtaagagtaa
    46681 ttctataaca tggcttgcgt atcgcttagt tgaggtggat tataataacc aaaaggcgtt
    46741 tgattcggta atagttgtta cggataggtt gaatttagat aaacagataa atgacaatat
    46801 caggaagttt atagatgaga agagcgttgt aggtcatgct aattcttcaa cagatttgaa
    46861 gaacctacta gttgatggca agaaaatcat tattactaca gtacagaaat tcccttattt
    46921 gcttgaaaaa ataggtactg atttaaaggg taagaatttt gctattatta ttgacgaagc
    46981 tcactcttct caaagtggtc gtgcagcagc ttcattaaat atggcggtat caggaagtct
    47041 gagtaacgat gatgaatttg aaattgaaga taaactgaac gaatttattg aagctagaaa
    47101 gatgcctgaa aatgctagtt tctttgcatt tactgctaca cctaaagcga aaacaattca
    47161 aatgtttgga aatgtatttg acctgtattc tatgaaacaa gctattgaag aagggtttat
    47221 tcttgatgta ctaaagaatt atacacatta tgaaaactac tataagattt ataaaactat
    47281 agaggataat ccaaactttg ataaaaagaa ggctcaaaag aaaattagaa attttgttga
    47341 aggacaggaa tttccaataa gagaaaaagc tgaggttatg gtaaatcatt tccttacaaa
    47401 tacagtaaat aagatcaatg gtaaagctaa agctatgatt ataactcaaa gtattttaag
    47461 agctattgag tattattaca tagttagtga attattaaat aactctaata cagggtatga
    47521 agctttagtt gcattttctg gggagaagga atacaaggga aaagtagtga cagaagccag
    47581 cttgaatggt ttttccgata aagagacacc agaaaaattt aagcaagata aatacaagtt
    47641 cttaattgtt gctgacaaat atcaaactgg atatgatgaa ccacttctac atactatgta
    47701 tgtagataaa gttttgaatg atgttaaagc agtacaaaca ttgtcgagac taaatagaag
    47761 tgcaaaatat aagatcgata cttgtgtaat tgattttgct aatgagccag aacatatact
    47821 agaagcattc caaccatact acaaggaaac aaaattggaa ggagaaacag atcctaataa
    47881 attaaacaat ttgctttcta tgcttgatag taagtatata tatgaagaag atgaggttga
    47941 taggcttgtt gatttattct taaagaatag tcctagaagt tttatagaca gcattgtcga
    48001 taaatctgta gaaagatata aagttttaag tgaggaagaa caagtagagt tcaagagtgg
    48061 agtcaaatcc tttataagga catataattt cttggcttcc attttaccta ttggacaagt
    48121 tgattgggaa aagaaagtta ttttctttga acagttgatt catagattgc ctacaccaga
    48181 aggtgaggat ttatctgagg gaattctaga atcagtggat cttgaaagct ataggctgga
    48241 aaagaaaaat acaatagata ttatcctaga agatgaggat ggcattgttg aaggtcttgg
    48301 tattagtgct ggtaaaaaga atgaagttga attagattct ttggaaaata ttgtctctac
    48361 tttcaatgac atttttggaa atattgattg gcaagacaaa gacaatgttg caagacaaat
    48421 taaagagttg ccagagatgg taatgaagaa cgaaaagttc aaaaatgctc taaaaaattc
    48481 agatattgaa aatatcaaga gggaatatca ctcagcactg aaagaagttt tcagaattat
    48541 aatggtcgat aacatggaac tttttgggca atggacaaat aattcgaact ttagtaaatg
    48601 gctaaacgat acgatttttg atgagattat gaagaaaaat aaaaggtaaa ggtggcgatt
    48661 aatttgggta gtatatcagg agttactaga agagatatta ttgatttgtt taaaaacgga
    48721 ataacagaag ataattggct aacagaagaa actatatatt acccttatta tggtcgcttt
    48781 gatatcgtag attttctcaa aagattgtat aagctagata cttggaaaag tggagattct
    48841 agactaaaaa atgcagagca agaaattacg atgcatacac gtaatggcga ttatccagat
    48901 gattggattt ttgctgatga aaggtttcac ttacttgatg gtgaagatag tgttttattg
    48961 ggctttctct gtgaagtttt tcaccctgaa gtaagagatg aaaataaaga atggaagaaa
    49021 tatttagaaa gaataaatag tctgttacgt gaagatggat acgaactaat tgcttctagt
    49081 aggctttctg ggagagatgt cttcacctgg agagtatata ttaagaagcc agatatgtat
    49141 attccttttt ctgaaagaaa taagtcattg atagtaggta gaaaaataaa ttttaaactt
    49201 cctaatgcag tgagacatca gctttttaag gtgatggatg agtatgatga aatattttac
    49261 ttaactgatg aaaccaattg gaattataca aaatatagta gtgattatgt tttagaagat
    49321 attaataaat tttatgtacc aaaacattat gttgatagta atcttgctga aataaaaaag
    49381 tttaaggatt ttcaagaagg cacttctcca ttcgtaattt ttgatgtcat tgaaagcttt
    49441 aataggcata ctacaaattc agaaggattt gaaaatgaaa ttaattctat ttttaatttg
    49501 aataatatta atgtagaact tagaagaggt gagatacatt cttctactaa taagacatta
    49561 tcattagatc cttctgtaaa aattaatgaa ataggcatag aagagttaat tagagctgca
    49621 gaagatttat atagaaaagg taaatactcg tatgctgttg aaaagatttg ggatgctttt
    49681 gaaaggatta aaacgtacta ttatcctgaa ttagataaaa agaaatcagc aaataagata
    49741 attgatgaac taagtaatgg gaatgacagc attaaaagta tgtttcaagc tgaatttaaa
    49801 tatttaacag atattggtaa ttcgtatcgc ataagacatc atgaaaaaaa caaaatagat
    49861 attaccgaag atttacacta tgaatatttt tataagaggt gtttagcttt aatatcagtt
    49921 ttagtaaaaa aaatataata aacgtgaggt aaaagatggt tgatttcaaa aagaaggttg
    49981 ctttaaaaag taaagcgaaa gttacaaatc caattgattt atataatact cttgatagaa
    50041 agagtattgc tgggccgctt cgacctgttc aagaatatgt tttaaatgaa tggtacgaga
    50101 atagaagaaa tgaaagagat ttaattgtta aacttcatac gggcgaaggg aaaaccttga
    50161 taggtttgct aatattacaa tcatcaatta acataggtga aggaccatgt atatatgttt
    50221 gccctaatat atatttggta cgacaagtgt gtgctgaagc taataaattt ggtattccct
    50281 tttgtgagat agaacaaaat aatgttttac caaatgaatt tttatcgggt gagaaaattt
    50341 taataacaca tgcacataaa atatttaatg gaaagtcaat ttttggaata ggaaataaat
    50401 gtgttaatgc aggaacgatt attctggatg attctcatgc ttgtatagat gtattaaaag
    50461 atgcatttac tattactatc gggaaaaaag aaaaagagaa tgtatataac aagatattaa
    50521 cattattttc agaagattta atagagcagg gggagggaag tttcttagat atcaaatctg
    50581 gtgaatatga aacattcatg actgttccat attgggcatg gattaataaa aaatcagaaa
    50641 ttctaaaaat actttcagat gagtcagcaa gtgatgaaat caaatttgct tggccgctaa
    50701 tgaaagataa aataatagag tattcatgtt ttatatctgg taataaaatt gaaataactc
    50761 catataatgt tagtgtagag caatttcgaa cattttcatc agcgaaacga agaatattaa
    50821 tgtctgcaac aacacaagaa gatatatttt ttgtaaaagg gcttgatttc tctactaatg
    50881 ctgttgcaaa tccaattaaa tatccaaaat cacgatggtc tggagaaaaa atggttttaa
    50941 tgccgtcgtt gatagatgaa gaatgtgata gagatttagt tgccacacaa tttgctgcac
    51001 tgacgaatgc accttttggt gttgtagcaa tcgtaccgaa tacaaaaagg gcagtatatt
    51061 atgaaaatat tggggcaaat ttccccaaaa ataataaaga attattcgaa gttattgatg
    51121 acttgagaaa gggaaaattt agtaatactg tagttataaa taacaggtat gatggtattg
    51181 atttacctga tgaaagttgt agagttttgg taatagattc gttgccttat ttgaatagct
    51241 attttgataa atacgaagag aaatgctgcc caaatagtga aataataaat aaaaaaattg
    51301 ctcaaaaaat agagcaaggt ataggtagag cggttcgtgg tgaaaaagat tattgtgcaa
    51361 ttatcgtaat tggttcagat attgaaaaat ttatgcgagg tattttaacg agaaaatatt
    51421 tttcgtctca aacacaaaaa caaattgaaa ttggttttga agttgctaaa atggcaaaag
    51481 aagagatttt gcaggatgat ccttcaatga aacaaatatt ttctttgata aagcaaatat
    51541 taaatagaga tgagggttgg aaagaatatt atgctagcga aatgaatgaa atcgaagatg
    51601 gggttttaga tacttcgatt tatgaaagat atgctgcaga gactaaatta gaaaaactat
    51661 tttctgatgg agaatatgaa aaagcatctg aagaaacgca gaagtttatt gataagttta
    51721 tacatgatga cttagagaga ggttggtatc tagaacagaa ggcaagatac actttcatgt
    51781 actctcaaga agaatcagct catttacaaa aagttgcatt ccaaaaaaac tctcaattgt
    51841 taaaacctaa cagtggaata atttatgata aagtttcatt tttaaatgag aatcgactaa
    51901 ttagatttag aaaatatatt agtaaattta acgatttcga tgagtttagt ctacatttaa
    51961 acgaagtgtt aaataacttt tcatttggaa tagatgccga taaatttgaa tctgcattaa
    52021 aaagcatagg agaaatgcta ggatacatta gtcaacgtcc agatcaagag ataagaaaag
    52081 gaccagataa cttgtggtgt gcctcaaata aaaaatatgc gatttttgaa tgtaaaagtg
    52141 aagtagatga aaatcgtggt gcaataaaaa aatctgaggc tgggcaattt aataatcatt
    52201 gtggttggtt taaaacggaa tatgatgaat atgttgatgt attgcgtgtt attgtcattc
    52261 ctacaaaaaa acttgcccgt gatgcagatt ttacagatga agtttttgtt atgaggaaga
    52321 atggggtgaa gaagttaaaa gataacatta aaaactttat gaaagagata aagaaatatg
    52381 aaatcgaatc aattactgat acaaaattgc aaaaatactt tgatatgtac aatcttaata
    52441 tcgataatct tattggtaat tatgttgaag agatttatca tctagaaaag taaagagagg
    52501 tagttttact tatctctcca tctccttacc catatatttc tcataatttt ttctgatttg
    52561 ataaagttct ttcatatcgg attttgtaga agaatattct tgcataaggg tattcttttt
    52621 ttcgtgtagt gaatccaacg ctgctaaaat atccttagaa tttggaagtt tagaataaga
    52681 ttttttgagg tctgaaaggg cgttctggta caaggtaatc tcagctttat gctcgtcaaa
    52741 aaatccctta tcagatggct ctttcttata tccctgatag atttggcgat aaattttgac
    52801 agtatgaacc tgctccatag ttgaagatag agcagaaatc ttgctatcta agatcttgat
    52861 ttcatcttgc agattttgcc ttttatcagc actttcctta ataaaatcat caagctgaga
    52921 aatggactta aagccttttt cacgcattaa aaccaccata ttggtagcta cttgaaggtt
    52981 gtgtttagta gcccaatatt catatccctt gcttgatttg accttttcat tattagcaat
    53041 atcaatgata tttcctacac gctttttgac agtataggtt ttttgattag cattctttgt
    53101 aatacgctct tttaatttat cttcagtata atcatctcca atagtctttg ctcttgtaaa
    53161 tctttctttg tccttatgct taaaggcaat atgttttccg tacttgattt catagtcaat
    53221 ggcagccatt ttctttagaa attcatccca atctttagac tgtttaatag ttctatcaat
    53281 atcgaattga agcctacttt tccaagaagt acctttctta aattgttcat tttcatacca
    53341 agacttccca ttagttttat attttttctt ataagtttcg tagaattcgt caataacaga
    53401 gagcttattt tctttgcaga gcttatcgct ttgataccta attttatgat agcttctttt
    53461 gttggattga tagcacttgc cagtaatcat attcacatta ttgaagatga tatggttatg
    53521 aatatgacct ttatcgatgt gagtagtaag cacaaattca tactgatcct taagtatttt
    53581 cttacataat tccataccaa ctttatgtgc catttctggt gttgcttcgc ctggtaaaaa
    53641 agattgaatg aggtgtcttg caagtacagt tcctttcaca ttattatctt ctctagtctt
    53701 taaaaactga gtatgagcag tagaagtaaa gcaattattt gtgcttatta aaatcttctc
    53761 atcagttttt tcttcgttag taatgtaagc aatagcaagg ttaagtgttg actttattgg
    53821 atgtatcttt gtaattgcca tttatacttc atctccttta tctgtagttc tattaagcag
    53881 tagtgaatga atttgccata tctcttttga taaaccttca atctgagatt tcatatcctt
    53941 gatgtcatcg ctataaataa cgccagttga attgacacgc ttagcgattt gattaacact
    54001 acttgcatat ctgaaaagta gtccttgtat ttctctgaat ggttgtaaat ctacaacata
    54061 aatatctgac tctgatacaa gctttcttaa aaaaaggctc atggttttgc acttagccat
    54121 cttcattttc ttttcaaaaa atgctttttc ttcttctgtt aagttgattt tgagttgaac
    54181 atttctaact ctatttgcca tattgattac ttcctttcat aaaatttctg gggttttagg
    54241 gtatgcccta acaagttaaa attgataaaa aaagaagcag ttccacgagg ggctgcttca
    54301 tcaatttttc gtaagtgggt acacacttac tgtgcttgct catatactaa tcgatcatat
    54361 actaatcgtg taagaacaac tttaagcata atatttatat tattatacca tagtttggaa
    54421 aaataaaaaa ttatatttca tcaacgtttc ataaaaggtg tgtagaatat tattgaaagt
    54481 agttataaac tgctattgtg taaaaataaa ggaggaataa aggaggaaaa atttatgaaa
    54541 aaaatagtat caagaggagc atgcctatta cttacgttat gtttgagctt aagtacagtt
    54601 gggtgtaaaa aaagtaatga aagcaataaa attaaagaag gacaatctat aagttctgaa
    54661 gaggcaggta tgtcaactaa tgagaaggat aaaaatgaaa catttaagcc atcagattat
    54721 actttaaaaa caaaaaaaga atatgtttat gaatatttag gtcttaaatt taaactttca
    54781 gacaaattta aaaagtatat ggatgataaa aagatagcga tgttagatga tcaaagtcct
    54841 atagataagg aactaaagta tgctttttta acttttaata aaatgaccga agagcaaaaa
    54901 aatgcagtta ttaataaaaa gggagatggt tatgaaaaat ggaagaatgg gcttaaaaga
    54961 attggaacta ttggtatatt cgaaaaaaat acatcagaag aaaaaatatc taaaattaca
    55021 aaatgtgata cccatactaa aataggagtt tcaagtgatg gaaaatacga ttgctattta
    55081 agcacaaata gcggatctga aagtaatctt ctggatgaat ttaaaaaaac tgagattcaa
    55141 attatagata aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa aactgattta
    55201 gaaaatacag aagcatttaa caaagaatcc gttaaagatt tacgtaaact atcaacaaag
    55261 gacataaatg gcaaggattt tacaagtaaa gattttgata agtatgatct tactatggta
    55321 aacgtatttg caacttggtg tacagcttgt gtaaaagaga ttcctgattt agttgaggtt
    55381 cagaatgaaa tgaaaagtaa aggtgttaat atggttggtg ttgttacaga tactgttgat
    55441 gataacggtg aaaacaagga agcaattgaa aagtcaaaat taatacagaa aaaaacaaag
    55501 gcttcatatc ctttcttaat gccagacaag acaaacttta atggtagatt gaatggtata
    55561 caggctatgc cagagacttt ctttgttgat aggaatggaa acattgtagg tgatacttat
    55621 tctggggcaa agagtgcaaa ggaatggaag caggtaattg aaaaagaatt agcaaaaata
    55681 aaaaataagt aaatgtaaag cggtgaatgg tatatggtta ataagttttt aaaaagccgt
    55741 accttatctt tagtaattgt ctttattagt atggctatga cttactatgg acatagtcat
    55801 ggagaggcta gagttgtatt ggagaaggcg ataagaattt gcatggagtg tgttggaatt
    55861 ggatagaatt aaaaaaatta taaacggtag tacaaaaaaa gataaatata aaataagaga
    55921 tcgttttaga catctatttc aatccgtttg gtttttagtt actaattctt attttgaagg
    55981 gtttaagaca ggtaaaatct atggtgggga cctgaaggaa gtttgtgttc cgggtatgaa
    56041 ttgttattcg tgcccggggg caaaaggttc ttgtcctata ggatcgctac aggcagtgat
    56101 tggaaattcg aactataagt ttagctatta tatagttggc tttatgttct tgataggtgc
    56161 acttatagga agatttgtct gcgggtggct atgtccattt ggattaattc aagatttatt
    56221 acacaaaatt ccatttttta aaaaaattga gacatttaag cttgataagc atttgagaaa
    56281 attgaaatat ataatattga ttgtatttgt aataatactt cctctatttt tagttgatat
    56341 tttagggcaa gggtctccat atttttgtaa gcttatttgt ccagttggta tgcttgaggg
    56401 aggaatacca cttgtattat tgaataaaag tatgagagga gctataggtt ttttgtacta
    56461 ttggaaggga gtaattttaa tacttacttt attactttca atagtaatat atagaccttt
    56521 ctgtaagtat gtctgtcctc tgggggctat ttattcagtt tttaacccga tttcagtatt
    56581 caggtataga ctagacgaag aaaagtgcat tagttgcggt agatgtaaaa aagtttgcca
    56641 aatgaatata gatccagttg aaaattgtaa tcatctagaa tgtatcagat gtggacgatg
    56701 taaaaatgct tgtcctgtgg atgcaattag ttgtggaata aaaaaataga gaggagctta
    56761 ttagaaaagt gataaaaaaa tagagaggag cttattagaa aagtgataaa aaacgcaata
    56821 gcatatatta caagaaaaaa aaatagaaca tttattattt ttgttatttt aacaatagtt
    56881 ctttcttgtt tgtattcttg tttaacaata atgaaatcaa gcaatgaaat agaaaaggct
    56941 ttatatgaaa gttctaattc ttcaatatca ataacaaaaa aagatggtaa atattttaat
    57001 gttaatcaat ttaaggatat tgaaaaatta aaagaaattg aagaaaaaat aattcaatat
    57061 gatggattag caaaactaaa agatgctaaa gtagttagcg gagaacaaag aataaataga
    57121 gaagatttat ctgacaaatt taagaatgtt gtttcactcg aagctacaaa taatactaaa
    57181 agaaacattt tatttagtag tggagtattt acaattaaag aaggtaaaaa tatagaagaa
    57241 aatgataaga attcaattat tgttcatgaa gaatttgcta aacaaaacaa tctaaaatta
    57301 ggtgatgaag ttgatcttga attactagat attgaaaaaa gtggaaaaat aaaaagtcat
    57361 aaatttaaaa ttataggaat tttttctggt aaaaaacagg aaacatatac aggactatct
    57421 tctgatttta gtgaaaatat ggtttttgta gattattcaa caagccaaga gatattaaat
    57481 aaatcagaaa ataataaaat tgcaaataaa attttaatgt attctggtag tttagaatcc
    57541 acagatttag ccttaaacaa attgaaagag cttaaaattg atgagtcaaa gtattccatt
    57601 gaaaaagatt ctaatgtgtt tgaagagtct ttagagtcag tgagtggaat aaaacatata
    57661 ataaaaataa tgacttattc tattatgtta ggggggatgg ttgttctttt attaatcttg
    57721 attctatggt taagagaaag aatttatgaa ataggtatat ttttatctat tggaacatct
    57781 aagatacaaa ttataatgca atttatattc gagttaatat tcatatcgat accaagtata
    57841 atatcttcct tatttttagg aaatgtatta ctaaaagtaa ttgtagatgg attcattaac
    57901 tcagaggact caatgatttc cggtggaagt ttaataaata atagtagttt tatgttaaat
    57961 ataacaacac ttggagaaag ttatttaata ttaataagta ttattgtttt atcagttgta
    58021 tttgcttctt cattaatatt aatcaagaag cctaaagaaa tattatcaaa aataagttag
    58081 gagaaaataa tggatatatt agaaataaag aatgtagcat atagttatgc aaattctaaa
    58141 gaaaaagttt tatcaggagt aaatcaaaaa tttgaacttg ggaagtttta tgcgatagta
    58201 ggaaagtcag gaacaggaaa atctacactt ctttccttat ttgctggact tgataaacct
    58261 caaacaggaa aaatattgtt taagaatgaa gatatagaaa agaaaggata tagtaatcat
    58321 agaaaaaata atatatcttt agtatttcaa aactataatt taatagatta tttatcacca
    58381 attgaaaata ttagattagt aaataaatca gcagatgaaa gtatcttgtt tgaactaggt
    58441 ttagataaaa aacaaataaa aagaaatgtt atgaaattat ctggtggaca gcaacaaaga
    58501 gtagctattg ctagagcatt ggtatcagat gctccaataa tactagctga tgagcctact
    58561 ggtaatctag acagtgttac tgctggagaa ataattaata tattaaagac attagctaaa
    58621 gatagaaata aatgtgtaat agttgtaaca catagtaagg aagtagcaga tgcggcagac
    58681 attattttag aactaagtgg taaaaagttg aaaaaagtaa ataaaatgaa tttggaggtt
    58741 gaataatgat aaaaaatgca tttgcttatg taactagaaa aagcttaaaa tcattaatta
    58801 ttatattagt tattttatct atgtcaactt taagtctcat tagtttatct attaaagatg
    58861 ctacggacag agcttcaaaa gaaacatttg ctaatataac aaatagcttt tttatggaga
    58921 taaatagaca agtaaatcca ggaacaccta gaggtggggg aaatgtaaaa ggtgaagata
    58981 ttaaaaagat atctcaaaca gatagtgtag actcctatgt aaaaagaata aacagtgttg
    59041 cagatttagt tgattatgat attattgaaa ctcaagagac tcttgcgaat caatcacctg
    59101 aaagagcgaa gaattttaaa agaacagtta tgttaactgg ggttaacgac tcatcaaaag
    59161 aaacaaaatt tgtatcagaa gcatataaat tagtagaagg aaagcattta gaaaatgaag
    59221 ataaaaataa aatcttaatg cataaagatt tagctgaaaa aaataatctt aaagttggag
    59281 ataagataaa aataaaatct aatttatttg acgctgataa tgagaaaggt gcagatgaaa
    59341 cagtagaagt agaaattaaa ggtctattcg atgggcataa tagcggtgga gtaagtgcag
    59401 tacaagaact atacgaaaat acattaatca ctgatgttca tacagctgct aaagtttatg
    59461 gtaatacaga agatacagct gtataccaag atgcaacatt ctttgtaaaa ggtgataaga
    59521 atcttgatag tgtaataaaa gatcttggga aattagatat aaattggaga gaatataatt
    59581 tgattaaaag ttcatcaaat taccctgcat tacaacaatc tatatcaggt atctactcaa
    59641 tttcaaataa attatttgtt ggctcattaa tatttgcagg agttgtagtt tcattattac
    59701 tattcttatg gatgaatgct agaaagaaag aaatagcagt attactatca ttaggtatat
    59761 caaaattaga aatttttggt caattcctaa ttgagatggt atttatatca atccccgcat
    59821 tcgttggttc ttatttttta gctcaatata cagctggcaa gcttgggaat aatatattga
    59881 ataaggttac tggtgatata gctaaacaaa tagctagaca atctgcatct agccaattag
    59941 gtggtggggc agaagctgaa ggctttaata agacattatc aagtttagat ataaatgtat
    60001 tacctaaatt cataatttat gtagttatat ttatgagttt agtacttctt gtatcattga
    60061 ttatttcttc attcaatata ttaagaagga acccaaaaga attattaatt gacaataatt
    60121 aaaagttttg gtgctttcta gcaccgaaag ttttttgact tttaaatatc aaataatatt
    60181 ctgtataata taagtgtata ggcttaagtg gaggtatata tgaaaatatt aattgttgaa
    60241 gatgataata tgataagaga gggaataagt gaatatcttt cagaatttgg atatactgtt
    60301 attcaagcta aagatggaag agaagctttg tcaaaattta atagcgctat aaatttggtt
    60361 atcttagaca ttcagatacc ttttataaat ggtttagaag tgttgaaaga aattagaaag
    60421 aaaagcaatt taccaattct aatcttgact gcatttagtg atgaagaata taaaattgat
    60481 gcatttacta atttagtaga tggatatgta gaaaagccat tttcattgcc ggtcttaaag
    60541 gttagaatag attctttaat taagaagaat tatggacatt tagagaagtt tgaatataag
    60601 gatctatcgg ttaactttaa tagctataca gctaaaataa atggtgaaga aatagatgta
    60661 aatgcaaagg aactagatgt attaaaatgt ttattggata atgatggaca agtgttaaca
    60721 agaatgcaaa ttattgatta tgtatggaaa gatagcgaag aaattcctta cgatcgagta
    60781 atagatgtat atataaaaga acttagaaaa aaattacaat tagactgtat aactactata
    60841 agaaatgtag ggtataaatt ggagataaaa tgagaaaatg aaaaaattaa agatatttcc
    60901 caaaatgttc atacaaatat tttctgttct tggaataata attatattag ttcattcatt
    60961 agtttttttt atttttccta aaacatattt ggagaccaga aaagaagaga ttcataataa
    61021 ggcaaatgaa atttccagta atatgaatgg aaaagaaata aaatatatag aacaaacttt
    61081 ggatctttat tctaagagta gtgaaataaa ggcatttata aaagaaaaaa aaaataataa
    61141 taatgaaata caaatcaaag ataatataga tgctaattta gaaagcaata gtaattcttt
    61201 gataattgaa gaaagagaaa taaagcttaa tgatggtaaa aaaacatatc tacaatttgt
    61261 ttctacagct gatatgcaaa aagatgcaaa agatttaagt cttaaatttt taccatattc
    61321 tttattaata tcaattttat tttctgctat tatttcttta atttatgcaa aattaataaa
    61381 aaataatata caagaaataa aaattgttac tgataaaatg atgaaacttg ataaaaaaat
    61441 gcgtttaaaa gttagctcta atgatgaagt tggagaatta aaacaacaaa ttaatgattt
    61501 gtattctact ttattaagaa caattgacga tttggaattt aaaaataaag aaattttaaa
    61561 attagaaaag ttaaaatatg acttttttaa aggtgcatcc catgaactta aaactcctct
    61621 tgctagtctt aaaataatac tggaaaacat gaaatacaat attggaaaat ataaagaaag
    61681 agatatttat attaatgaat gcattgaaat cgttgatagt ttaacaaaaa atatttctca
    61741 aatattatca gttcattcta tagaaaattt gaataatgat gaagaatatt tgaaaataaa
    61801 tgatacaata gaagacatat taaaaaagta tgaaatatta gcaaatcaaa agaaaataac
    61861 tgtcaataat tatatattag atgagaatgt ttatatagga aaaacagctt taaagattat
    61921 tctatctaat ttgattagta atgcggtaaa atatactgat gtaaatgggg taattaatat
    61981 tgggatagtt aatgattggt tatatataga gaattcatac ggtaacaata aaatttcgaa
    62041 tatggataaa atatttgatg ttaaatttga tttaaataag gaaaatagta atggattagg
    62101 tttatatatt gtaagtaata ttctgaataa ttacaatata gaatataaag cattgcagga
    62161 tgaagaattt tttatcttta aaattaaaat attttcttaa ataagatata attttgcaat
    62221 agttattgaa aaatagagac ttattattag ccatggaagt aaagactttt ttcttattga
    62281 aagtgaacag agaataattg atacaagaaa tcgggatttg aacacgctaa taaaaatatt
    62341 gttagtaaaa ctatatgagt tagtcttttc ggatgcagag attgtttaaa tatttgatag
    62401 aatagaaact taaaaagttt aataaaactc aattatataa gtagtagatg aatatgatgg
    62461 ttatttagat tattgataaa atggaggaaa taaatatgga agcatctaaa cgtttaatgt
    62521 tagaaatatt tagaattcaa tatattcctc ttttttataa tatgtatagt agatgattgt
    62581 ttcaggtgcg aattgaaaca atcaagttca tataaagaaa tatttttatt ctttcatttc
    62641 agaggacttc ttatgccaag atgcagaagt cctccttttt tatctgaaaa acaagcagat
    62701 aaaaaagaga tggaggaaac tttatggcaa aagagtatta tctttatatc agaggacaaa
    62761 aggtaaaagt cagtgaagat atatatatag tctactggcg agaaaaagaa cacgagaagt
    62821 atttagagca ggtggacagg aaaaaccact tgctcttttt ttcagcattg gatcatgacg
    62881 gacactttac agataatatt gttgatgaaa gtgttgatgt agaaaagatt gttgaaacac
    62941 agatgatgat tgaagcagta agaagtgcta taacaaggct caatgcagaa gaaagagata
    63001 ttattgaacg cttgtatttc aatgatgaaa ctgttcgttc agtagcaaag ctcaaaagta
    63061 ttacacatcc ggctttaatc aaaagaagaa acaaaattct tgaaaagctg aaaaaattta
    63121 tcgaagaact ttaaaacttc ggtaaccaaa gggggcaaat tttccttatg taaagtgaag
    63181 gggagtttga cctcctttac tttcttgagt aggagatacg cctgcttatg gcaaatagga
    63241 aaatgtaaag aaaacagtcc ctttcaaata ttgctctttg acaactgaat agaccaaggt
    63301 aggactttat ctacttactg agagttatga cctacgccaa gacttttctg ctgatgaaaa
    63361 tcggttcaaa tgaataccgt cataatgccg aggttagaag aaaatgagcg atagatagga
    63421 atacataaaa gataagcgtg gcagcttatt tcgatgatgt aagtggtgat aatgatactt
    63481 caatagttta agctgcctcg tctgaaacaa ggggcggagg tgagattcct atgtagcagc
    63541 caatgcacct accaatgtca taaaacttag taagaaaaat aatatttggt tataaagaat
    63601 ttaaagtgga ggaggtgtat tagtggataa tgactggaat ggattggctg atttgatagc
    63661 aaatctgatt acaaaatatg ctggagtttt agatttagag aatcttccaa atcctactcc
    63721 agtaaaaaat acagaggata agaataaatt tgatatagct aaaagcacgg attgagaaag
    63781 agaaataaaa gtgctataat atacttgaga ttaaagtcca aacctttatt ggtaaaaata
    63841 tataaatata taataaggat attgaggtta atgctatgtc aaaagaaaaa ataaaagtat
    63901 atctctatac aagagtatct acatccatac agatagatgg ttattcttta gaggcacaaa
    63961 aatcaagaat gaaggctttt gctctctata atgattatga gattgttggt gaatacgaag
    64021 atgcaggtaa gtctggaaaa tctatagaag gtagagttca gtttactcgt atgatggaag
    64081 atataaaatc cggaaaggat ggagtgtctt tcgttcttgt atttaaacta tcgagatttg
    64141 caagaaatgc tgctgatgta ctttcgactt tacaaataat gcaagatttt ggagtaaatc
    64201 ttatttgcgt tgaagatgga attgattcat ctaaagatgc gggtaaattg atgatttctg
    64261 ttttatcggc agtggctgaa atagaaagag aaaacattcg tgtccaaaca atggaaggtc
    64321 gcattcaaaa ggcaaaagag ggtaaatgga atggtggatt tgctccgtat ggttatcaac
    64381 ttattgatgg gaaattgata ataaatgagg aagaagcaat tgcaataaga actatttttg
    64441 atcaatatgt aaatacaagt attggagcta atggtctttc taaatattta gaaaatcatg
    64501 gtataaggaa aataccaaga caaaatggga aaaatccatt gtttgacgca ggtcttataa
    64561 gaaaaatatt aaagaatcct gtgtataacg ggaaaatagc atttggaaga agaactttag
    64621 aaaaagttca tggtacaagg aatgaataca gacaagtaga gcaggacgat tatttagtag
    64681 cagaaggcat tcatgaagca ataatcccag atgaattgtg gcaggctgca caagttaaac
    64741 tgaaagcaca agctaaaaag tatgaacatg taaataaggg caagaacatg aggacacatt
    64801 tattatcagg aatagtaaag tgtccaatat gtggagctgg aatgtttggg aacaaatcta
    64861 taaaatataa aaaagatggg acgaagtata aagacttcta ttactatggt tgtaagcata
    64921 gacttatgaa tagaggacat aaatgcacct ataacaaaca aattagagaa gaattgttag
    64981 acgatgctgt tgctgaggta ataatcaagt tagtaagcaa ccctaaattt gcgtctatga
    65041 tgcaagaaaa aattaacatg aaggtcgata catctgctat tgaaaatgag atagataatt
    65101 accaaaaaga gctgagaaaa agtcattcta caaagtttaa gctaattgaa gaaatagata
    65161 atttagatgt tgaggataaa cactataaga gaaggaagaa tgatttagat gatagacttt
    65221 atcgtatgta tgataaaatt gaggaattag aaagtcagtt aatagaagct aaagcaaaaa
    65281 aagaaacgat agaggttgag aaacttactg gtgataacat atataaagta ttgatttatt
    65341 ttgacaagct ttataaagtc atgaacgatg ttgaacgcag gcagttaata gaagcactca
    65401 tttcagaaat tcaaatttat gaagaaaagc agcctaatgg acaatggcta aaatccatta
    65461 cttttaagct accgatcatt gataaggatc taaatataag tttggacaat gataagcaag
    65521 ttgaaactgt agctaagata atcaaacaat agtaatgttt atatagatta tatactaata
    65581 atatattgta tttgccaccg atttgccact gtaaagcgat tttggtggca aatttagtct
    65641 acaatgttat caaaaatctc taaagtttta tttttaattc tataaaactt attataattg
    65701 taattttaaa agaaatcgtc aatctacagt agccagattt tatacaactc atttttttca
    65761 tagaataatt tacataatct atagttgaat attatttaat atttttatat tataattcat
    65821 tagcaaatat aggcattaca aatgttgata aacatgtgaa agcacatatc cacttataat
    65881 tatttttttt taaaccataa aatcacctct tcatttgttg tatgttttta taacaaatga
    65941 ggaggtgatt tccaaacaat ttaaaaggtt tttgtatatt gactaaaatt tatttttatt
    66001 atttataatt cttaataata catatgtacc tactgattcc atattaaact gtataatttt
    66061 tgttcatctt tttttataat taaaactaca tagttttcat aatttctctt atatatatac
    66121 ttatcatctt ttttaacttc taacttatat tttttaaaat cttcttcaat tttattttta
    66181 ttacttttat taatgttaac agaatctctt ttaaatttat caatatattt ttctatttca
    66241 ttaatattat ctttgcttac aactatacca ccttttaaat taaaaacttt tttgaaattt
    66301 ttttcagaaa atccttgaat aaaataactt tgtccatctc cattaatact atcttgagta
    66361 tcaaatactt caatttcttt gttaggtatt ggtatatcaa aattccaaga ttcctttagc
    66421 attgaaatat tagctgtttt tcctaaaaaa agattatata ggatacttcc taataacaaa
    66481 acatctatta atagtagtgt gattaataca atttttctct ttttcatttt tcgttcacaa
    66541 ccttttgaaa aaacatgatt taattcatca tttgaaggca tgtcttcaag aataattttt
    66601 gttgtgatag gaatgctttc ttgaaacata tcatttgtaa tatcaaaatc ttttctttga
    66661 ttactcaata tccatttctt ctaatatttc ttttgatttt ttctagtaca ttgaatactc
    66721 ttatatacat tctcctcttt taaaattaaa agttataaaa gaataagtta tataaaaaat
    66781 tattatattt tttaatatag cttatttgca aatttataat tttgttaatc taatccttca
    66841 aactaaaaaa tcatttatgt ttgcagaatt tcattttaag agatgtttat tttcacatag
    66901 ataagagaat ttaagttctc atatttatta tttgatacaa atttacatct ttattatatg
    66961 tattttaaat aaaaaataaa attatgaata ctagaaatct atcttaatta gctttctaac
    67021 ataaggctat ttaaagtaga taaaactaaa aagggttctc tagaaaacca aatctattta
    67081 attatactta cttactaata ccatcacttg atgcaaacaa gattctacca cattttctcc
    67141 gtactctagt ataaaatcat ctagttgtgt atctataaaa tcacaataat catatgaact
    67201 accaatataa gcagtcaaca tatgtgtaca tctatccatt ttatctctaa acatatatat
    67261 tattatcccc ctaaaaaact ttcataaaaa caaaaagaat atctcttttt ggcaactggc
    67321 tgacataact catagtttat tttataagcg ttagtcccta tagctttgcg tcactaaatt
    67381 tctctagttt tgccgattta gttttattct acaattacaa taatacaata gaattgctta
    67441 ttattcaata tacttatttg aaaattaaat aaatttacaa atatgaacgt tattttatat
    67501 caaaaaaata gactatgcat gtagaatata tggaaaaact tgattgtagg ttttgtatga
    67561 aaagacagtt gttattaatt taagtgagat aaattgagca acaggcataa aatgagaaaa
    67621 caaataatag cagttaattt ttattattaa tactaggtga acattaaatt ttaaaatacc
    67681 actagatttt atagaataag aaaatgagac aaacataaat atatgaatat attaaattat
    67741 acttgaacat acttatattt tatgataaaa tttcaataat aattattagt gaaggagtgt
    67801 cttgcctgat gtcagagtta tatataatgg taaccaacaa ataaatagtt ggagacgcat
    67861 atgggtcctg tttaatcaga atttttaagt gtatttttga tacatttgat tgttgtgtct
    67921 tactctgcct ttatagataa ctaacattag acaacaaata ataatattac atattacttt
    67981 gttaaattgt ggtgtagtgg gctttaatct gctacacttt tattaatttt acacagtatt
    68041 attttgaata taattatgtt gaaatagaag atactaaatt ttctagtcca caggggtttt
    68101 ctgtttttgt gtaggtaaat atgtatacca cataaaaagg cagatataaa tacctgtgga
    68161 ctttttttgt agaaaataca agatacggag gaaataaaaa tgaattataa aaatagaaaa
    68221 cgagcttatt atcaggaaaa ttcctgtatg gataacttta tttgcaaaaa ttgtggttgt
    68281 cttgtagttc ctgaaggagc tggtacacaa catagaaatc attgtccaca ttgtttacat
    68341 agtcttcatg tagatatcat tcctggtgac cgtgctgcag attgtgatgc taatatggaa
    68401 cctattggag tgtgggtgag aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc
    68461 tgtggacatc ttagttctaa tcgtattgca gctgatgata attcagtgaa attaatgtca
    68521 attgcattaa aaccactggc acagccccct ttcccactag aaagaatcat tgaaaagttg
    68581 gaaaaggagg aaacagaatg aatggtatga aacaaatcaa tttacaagac tatggtctaa
    68641 atagaaattt tattactcaa agtatggaat atcatgattt ataccttgct cggattatct
    68701 cgcaggaaaa gaacttttat caagctgtat gtgtaaatgg tgtactgttt gcagaggttt
    68761 caggaaagct gcgttttgag gctactatac aatctgattt tcccacagtt ggtgatttcg
    68821 ttatgttgga tcgttgcaat aataaaaatg gtaatgctat tattcatcat gtgcttcata
    68881 gaaaaagctt gtttgtgcgt aaggctgctg gaaaggtaaa tgaggagcag gtggttgcag
    68941 ctaatattga cattgttttt ttgtgcatgt cactgaataa ggattttaac attcatagat
    69001 tggagaggta tctgtcttta acatgggaaa gtggagctat cccagtagta gtacttacta
    69061 aatcagactt atgtgacaat gtgcatcgta aattacaagt agttaattct atagccttgg
    69121 gtgtagatgt agttgttacc tcatctatag agccagatgg ttataaacag atttctaagt
    69181 atattaaacc aggctgtact gttgccttac ttggttcttc aggagttgga aaatcttcat
    69241 taattaattg tctgatgggt ggagaatttt taaatacaaa ggaaattcgc atagatgatg
    69301 ataaaggaag acatgccacg actagacgag agctattttt aatttcaggt ggtggtattg
    69361 ttattgacac accaggtatg agagagttgg gaatgtggga tgtttcagaa ggtttagata
    69421 aaagttttgc agatatagaa gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca
    69481 cctcagaacc aggctgtgct atctgggaag ctattgctca tcaagaactt tcgcctaaaa
    69541 gatgggaatc ctatcgtagg cttaaaactg aagcagtatt tgcagacaat aaaagcgaat
    69601 acttagcaaa aaaagaaaag aaatttaaaa atatctcaaa aataaataag aagattagaa
    69661 gataagtgta gtttttttgt tcattctatg agtctatata ctattaatat attaaatttg
    69721 ccatcaattt gttactttaa tgtgattttg atggcaaatt tggtttgtgg tattatttaa
    69781 agcaatttat taattttttt aataaagttt gattttttat atgatgatat atacatgtga
    69841 taatgtatac atatttgtta cgataggctt ctttataaag aggtcttatg taagataaga
    69901 aaaggcatat ttatactaat acataataga gtatatgctt atgtatgata gagatgtttc
    69961 ttaagagcaa aactgcaata ttgctaattt ataaagtaaa aatatatgaa ttataaaata
    70021 caaataatat atttataaaa attattgttt taatttataa taaaaatttt ttgttgaaaa
    70081 taatgctcga acctataaaa ctattaacta atatttgtaa atgtatactt ttgatgataa
    70141 aatataatta agttaagttg gtttctataa acataaattt attttaggga ggtgaaataa
    70201 atgaaaatat ctagtcaata tagaagtcaa tattcattta gatatgaaag taatataaat
    70261 aatacaagaa taaatgaaaa tatggttaag aaaaatgaaa ctgtaggaaa agacacttat
    70321 ttatctaata ttatgaaaca aaagcaagaa cttaatgata gaattagaga tttaaaatat
    70381 agacaagagg tttatgctaa aaaaataaat gacgcaatta agaacttatg taaatcagaa
    70441 ataagagaaa caactaataa cttttctaat atagaaatag gtattaaaaa tagcattata
    70501 gaagataaaa ataaaagtac aatgttagat gaaaattcaa cttatctaaa tacaaatgat
    70561 gaaaaagaat ctttaatcac taaagagtct aatgaaaaaa ttgaagaaga aatattaaat
    70621 gatgaaaaat tagaagagtt agaacaaaaa aaggattata aagaggattc caataaaaaa
    70681 gagaaagtat ctgaggactt atctttagta ggtaaaactc gtgaagagct tgaaaatatg
    70741 cttaaaaatt ttataaattt aacacaagaa gaaataatga aacttgagtc gagaatagaa
    70801 aagttagata aagatgctga agaatacaaa caaaattcaa agactaatat atttgataaa
    70861 acagatgaac aaaaaaaaca tataaatgta ctgatttaaa caatatgttt taaaaaaata
    70921 agtttattat ataatgaaaa tataaaggta caatttcaat acacttctat taaaccaata
    70981 atttatgaat cagatttaat ttcaatttac taataaccat atatttatga gttaataatt
    71041 ttatgtttta aaaagaatta aataaggggg agtagtatga agaagaatat attaactact
    71101 atgattacca tgattattat attgatgatg agtatgacag taatggcaga gccatatcaa
    71161 gaagatgaaa catctagctt gaaaaaacag agtgcaaatg aacgtaatca gactaatata
    71221 ggcacaatgg atggcagagt gcaaacaaat gcaataagta aaggtgctaa ttatgaccct
    71281 aaatatcctc ttaaaggaat gttaacacaa cttggtctta atacaacagg taaagaggga
    71341 tgggatttac tctatggagg aaatcccaat ggagttgagc ttcgagcaga tggtaaatat
    71401 tattatataa ataaaggtat ggaagggcta ggattttatg cggcagcatt gtcaggtggc
    71461 tcatatcaga gaataggctt tggaaaactt gaccctatag aagtaatagc cgatggaaat
    71521 tggatttctt acaaacaagc tcaagataca cttacagtaa tcaggaattt tttaaatagt
    71581 tttgactgga gaaatgcatc tgagatggaa agagctaata gagctgcaaa attggtaacc
    71641 gaagcaaaat atgcagactc taaatattgt aatatagtct atggtaatct ggttgataaa
    71701 cgtggtgtat gtggaagttt tgcaagtagc tttcatttat taacaaggtt aatgggaatg
    71761 aattcactat ttatattaaa tccgtcatta aatcatgctt ggaactatat acaaattgat
    71821 gggaaatggt ataggtttga tgggtctgaa atatcagctt ttggtggatc attagacttt
    71881 gattaccgta aattaaagga tgcaacaaga gagatgacta cttactatga tgctaaggca
    71941 ttgtctatat taggatttaa tcaataaaaa taggggaggc ttcttataga gccttttttt
    72001 attgatttga aatttaataa ggaattttat atgaaaattt aatcaataaa agcaaaaaag
    72061 gctctttata aagcctttct aagtgttttt ttaagtaaaa ttttttatat caacatcagt
    72121 tttaatcaaa acatataaca ttttggactt acttttaaaa ataaaattat taaaatataa
    72181 tattaatcat gttaataatg tttttagttt attatattct ataatagtgg tcatgttgac
    72241 tattgtagtt ttattaagaa tagttggttt atataaatat atgcaaagat agcttgttgt
    72301 ttactttact tattagtata taggacaaga tactctaata aatttgccta ttttaatatc
    72361 ttttgtacta aaaccaacta aagtttcctt tattatttcg ttttgaataa tacaaagcat
    72421 aatccgattg tttgtataaa gactggaaag tatcagagtt attttctaat ttagatatac
    72481 ctatagaaca agaattactc tctaggcata attctaaaac cttctttgcc ttattttcaa
    72541 cattctcttt tgattgatta ctatattcaa aaacagcaaa ttcatctcca ccaagtcttc
    72601 caataatagc atcatttacg aatactttag tcaaaatatt tgaaaaatgt attagtattt
    72661 catctcctcg aacatgacca aagatatcat ttacctgttt aaaattatct aaatcaaaga
    72721 tgtaaaagta tccatgtgaa acagttttaa gcatttcttt tatgtgtact tcaaaaaaat
    72781 gtctattgta tagtccagtc ataaggtctt tttctgattt tagtttaaat tcatttgcca
    72841 ttgctagtgc ttcttgtgct ttttcagtta ttgttttagg gtaatactca tctttcattt
    72901 ggtcaaagtt tggaatagaa tggtgaacat ggataagttt aatcatatca tctgtaactc
    72961 tgtataccat agtaaatcga gtatccattt ctacatagat atctttttct aggtaatctt
    73021 tttctattac ccaaagagta ccatatacta taaaagcttc gtctgtaact ttggtacatg
    73081 aataccactc atctataatc tcaaagtcta tttgctctga ttctgtttgg tctttttcaa
    73141 gacctataat aaattcttct aatgtttcgt aaaattcatg ttttcctgtt ccaattaatg
    73201 caaggttgtc atctaataag gaaataataa atttaaggtt ttcttcttct ggatttaaaa
    73261 tataaatatg ccaaattttt tttgataaat ctcttaattg tttttgtatt gtattatttt
    73321 ccatagatta cccccttaag atttacagtt tatattttaa tgtattataa gttgaactct
    73381 tattacaata ttattataat atagaactaa agaaattaca tcctgtatag gtaaaagtat
    73441 atcttttaca ataaaaatat tacagtaaaa taaccagttt taatatacgt attatatttt
    73501 taatttataa acgaatatta taaatgaaat ttaaattcaa ccctgaagaa ataagctaaa
    73561 tgtattagta tataaatcca tacttgaaca taaataacct ttatgatatg atatcaataa
    73621 taatattatg agaggagtgc cttgatgtcg gagttatata atggtaacca acaattaaat
    73681 agttggagac acatatattc tgtataatca gaaattttaa gtgtattttt aatacattta
    73741 atttttgtgt cttattctgc ctttatagat aactgacata tgacaacaaa tagtatttaa
    73801 aatatatatt actttgttaa attatggtgt agtaggcttt aatctgctac acttttatta
    73861 attttgtata gtattatttt gataactatt ttgaaataga gagtattaat ttttctagtc
    73921 cacaggagtt ttctgttttt gtgctgttaa atataagcac agaaaaaggc agatatatac
    73981 ctgtggactt tttttatata aattatgaaa tatggaggta gttaaaatga gtataattga
    74041 acttaatgaa ctgactaaat cttatagaag atttgagaaa aaggcaggat ttatggggag
    74101 tgtaaagtct ttatttaata ggaaatatac agaaaaaaca gctatatcgt catttgattt
    74161 aaagatagaa gaaggagaat ttgttggatt aataggtcaa aatggagctg gcaaaactac
    74221 tcttattaag atgttaactg gtattattgt accgtcttct ggagaaatat ctgttatggg
    74281 ttattaccct aacaaattag aaaataattt taaaaaacaa tatgctgttg ttatgggcca
    74341 aaaaagtcaa ctattctttg aattgacacc agcagatacg tttcttttat ttaaagaatt
    74401 atatgatata tctaatgaag aatataaaaa aaacttggat tactttatcg aattatttga
    74461 ggtatctgac tatttaaatg ttcaagttag gacgctttct ttaggagaaa gaatgaaaat
    74521 ggaactaata gtagcccttc tacataatcc aaaaatatta tttttggatg aaccaactat
    74581 aggcttagat gctatagccc aaaaacaaat taggtatttc ctaaaagaag taaatgaaac
    74641 aaaaggaact actattatac ttacttctca ttacatggaa gatattaaga gtctctgtag
    74701 tagaagtatt gttgtagaaa atagccgtaa aatatatgat ggttctacag agaaactatt
    74761 taatcatttt caaaaatata aaaaaataac agttagtttt tccaatgaaa ctaacattgt
    74821 atttccaaaa gattgtaaag ttgttgaaca aacaaatcat aagatatcag taattgtgcc
    74881 aaaagaaaga ggaaatgaaa cgattaaatg gatttttgat aaatatgatg ttaaagatgt
    74941 aatgattgaa gaagaagata taggtagtat tgtagagaga atatataaga aaggaagtga
    75001 tgagtattat gagcaactat aatgaagcaa gttcaaatta ttcaatgttt tataaattta
    75061 aacagagtat tagaaggtat tatgaagtag ccaaaattaa ttttaaagtg caacttatct
    75121 ggagatttga tgtggtggtt aatatggtgt ttacgcttgt aaaaatttta tttgcttata
    75181 ttttatggaa agcaattttt ggtgaaaata aaatagtctc tggttttaca tttaactcta
    75241 tgttatctta ctatataata agctcatttt tagcaggtat tgatatgtca aagagtacca
    75301 gtgaagagat atgtaatcgt gtaaaattgg gcaccttttc aaagtacatg attattccaa
    75361 ttaatgttca aaattatttt attgctaaaa attttggaat gacatgtttt tattttgtat
    75421 ttaatatgat ttctgcattt gtatggattt ttatatttaa aatagatttt gcctttacat
    75481 caaatccagt tcaaatatta atttcaattt tgatgatttt tctagggttt atttttatga
    75541 tgaaactcca cttctttatt gggattttgt catttaaatt tcttgagatt agttttttta
    75601 gaatgataat tgaaaatatt gttgaattta ttacaggaac aatgatacca cttgtactct
    75661 taccagaaac tatagtaaca gttatgaagg tatttccatt ttattataca acatatttgc
    75721 catctatgtt actaataggt agaaacagca atgaggcaat acaaggatta attattctag
    75781 cattatgggt tttatttttt tctgtagtaa ataaagttat gtataaaaga atgagaacat
    75841 tatttgatgg ggtgggtata tgatgaaaaa aaatttgaaa ataatattta tgttattaaa
    75901 aatgaaatta tcaaagatga tggtatttag atttgatttt tttggagcat tttttgtaga
    75961 tagttcatta tttatattac aacttctaat gtttaatagt atatattctc atgtagactc
    76021 aataggggga tggcaacaag gagagatgtt aatatttata ggaactttct cattaataaa
    76081 tgcgataaat atgacgattt tcttttttgg tatttatgat attccacgaa aaatccaaga
    76141 aggagaatta gactactata ttacaaaacc attagatcca ctatttaggt taacatttga
    76201 aaatattaat attggttcat caccattaat tctagcaagt atacttattg ttttatatgg
    76261 aatatctgag ttaaactcag agataacttt agttatgata ataatatata ttttcatggt
    76321 gttattgatg acattacttt tttatgatgt atgtatcata cttagaacag tttcattttt
    76381 tgttatatca tcttctgcga taatgagatt agaagataat ttattgccaa tgaatatgaa
    76441 aattcctggt gtaatatatg aaggtgtttt taaagtatta ttctatttaa tcttaccata
    76501 tggaattatg tcgacaattc cgactcaaat tttggctggg acaataacaa ttaatggatt
    76561 aatatactca attttaattg tatttttgtt tacattgttt atgtggagat tttggaagtt
    76621 aggtatgaga cattataaaa gtgcaagtag ttaataaatt agtaaaaata aacaatgtat
    76681 aactatatat aaatacgttt catatgacac taaactatgt gaacaagtat ttgcgctaaa
    76741 ggcactgaaa gctataaaat tattaaagta agaatttaaa agtttcttta gtagaaatat
    76801 aattgtgtaa aaaagcaaga ttaattaaaa ctatatccaa ataattagat ataattctta
    76861 tgcagaaagg aggtctgtaa atgtctaatt gtttggaata tattttaaaa ataattaatg
    76921 tacagaaaaa taaacaaaat gagtgatgta tattattcag aaaatcatga acaagataat
    76981 ctttatgagc aagataattt actggcacaa agatttaatt tatttactct attgaagttt
    77041 gcatttccta ctatttttat gatgattttt atggggttgt atacaatcgt agatactata
    77101 tttgtatcaa gatttattaa tacaaatgct ctatcagcaa ttaatattgt atgtccaatt
    77161 attaatctta tagttggaat tggaactatg cttgccactg gaggaagtgc tattgtagca
    77221 cgtaagatgg gaaatggtga tattgaggga gcaaataaag ctttttcttt aatcgtatta
    77281 tcaggagttg taattggggt aataattact ggtataggta ttgtatttat ggattatatc
    77341 attatatggt taggagcaag tgaagtttta attaaatatt gtcgttcata tctttttatt
    77401 atcttggttt ttgcttctgc aaatatacta caagtattat ttcaatgttt atttgtaaca
    77461 gctggaaagc ctcaatttgg attatggcta gtgttaggtg ctgggtgtgc taatataatt
    77521 cttgactatg tgttcattgt tctgttacaa atgggtatta gtggagcagc acttgcaact
    77581 ggagtaggat acctaattcc taccatttca ggtatggttt tcttttcaaa aaataaaggg
    77641 ttacttaaat tttgtatacc tagactagat aaaagaatat tatttgaagc ttgttttaat
    77701 ggttcatctg aaatggtggg tcaactatca acagcagtta ctactttttt atttaatgct
    77761 atgatgatga aattaattgg tgaagatggg gttgcagcta ttacaataat tatttattct
    77821 caatttcttt taacttcgct ttatataggt ttctcaatgg gtgttgcacc aataataagt
    77881 tataactatg gaagtgaaaa taatacgcag ttaaatcatg tttttaagat ttgtatatta
    77941 tttatttcag tagcatcttt attggtattt atcttttcta tatcatgttc accaatcatt
    78001 gtagagactt tttctcaaag aggaagccga gtttatgaaa ttgcaaggtc cggattttat
    78061 atattttctt ttagttttat tttttgtgga tataatattt ttacctcatc tatgtttaca
    78121 gcattatcaa atggaaaggt gtctgcaata ttatcttttc tacgtacatt tggatttata
    78181 actataggtt tattgatatt gccaaatata ttaaatataa atgggatatg gttagcagta
    78241 ccaagtgctg agattttaac cttattttta actgtttttt gtatatttaa gtataagaat
    78301 agatataaat atatgtagat attaaataaa tagcttagat agatatttta tgttttaata
    78361 agtaattcaa ttagtggtag atatatacta ttgaaaatta aagttataat tgatataaag
    78421 aattaataaa aataacatat aaaagtttaa aagggagttg aacttaaagt ttgattagaa
    78481 aaagtcaatt ttaagacaac tccttttatt ttactattgt attaatctgt atctgtaagt
    78541 gtattagtct aatattataa actttggatg aaattaattt tctataggtt ttaatgtatc
    78601 atacacttta ttttaaagaa acttatctta attttcatag tgattgaatt tgtctattag
    78661 agatgttact attagtaatg taaaagcaag agtagtgaat ataaaaaaca agatagtgat
    78721 actgcaagat tagaatataa taaattttta tggagagatt tcaatatgga tacatgtttt
    78781 aataaaaaaa tattacttgt tgatgatgaa aaggatatag tagatttaat tgaagaagta
    78841 ctcataaatg atggttttaa aaacattatt aaagcttata atggtctaga tgctatttcg
    78901 ctgtgtaaga tagcatgtcc agatgttgtc attcttgata taatgttacc agatatagat
    78961 ggtatagaag tgtgtaaaaa aattcgagag ttttcatact gttctattct ttttttatca
    79021 tcaaaaaatg atgatattga caaaatacta ggtcttagta gtgggggaga tgactatata
    79081 acaaagccat tcagtccaag agaaattgct tttcgagtta aagcgcaatt acgtagacaa
    79141 cagtatcaaa gcattgtacc atcagatagt gaagttatta aaataggaga tattactatt
    79201 gatatagaag gaaaccgtgt atataaagat agaaatgaga ttgagttaac tggaagggag
    79261 tatcatcttt taagctacat ggctaaaaat gtaaataaaa ttataggaaa agaaagactc
    79321 tatgaacaag tatggggagt atatagtagt atttgtgaca atacaattat ggtgcatata
    79381 cgacatataa gagaaaaaat tgaagataac ccctcaaatc ctaaaatact tataacagtt
    79441 aaaggactag gatataaact tgtgaataga atcgattaga atagatttta atattaatga
    79501 agatatctca agttgaattt ttagttaatg aggcattctt ttatgggtga aatcaaatgt
    79561 attttcgtta tctcaacaat gaagaagagg tctatctcta ttttgagata gcctcattaa
    79621 gtttatatcc tggtaatgta ctttatgttc taataaaatt tttttagttt atttgcagtg
    79681 aaacattaac tagaacatta aaatttgcat tgaaatacca attaaataat attgtatatt
    79741 gtgtataaac ttatactaat tgtttttcta tattaaatac tttatcaatc caacctttat
    79801 aaaatttttc ttcatgggaa actaggatta tacttcctct aaactgtttc aaagcatttt
    79861 gtaaagcatc ctttgtctct gcatcaaagt gattggttgg ttcatccaga ataagaaaat
    79921 tacaaggaga taacatcata caacataatt taaccttaga ttgttcacca ccacttaaag
    79981 tagatacact ccttgaaaca tgctcttcct tcacaccaca tctggctaga tgacgtctaa
    80041 tctcttttgt atttaatttt ggatatttat ctgctactat ttgaagtggg gttttatcag
    80101 gattttccca ttttaaatct tgctcataat aaccaatttt aacttgttct gaaaactgga
    80161 aatctccaga tatacgagga atatctttta ctaatgtctt taacaaggta gatttaccga
    80221 ttccattaaa accagttata actatttttt gacctccatc aacagaaaaa tttaattttg
    80281 gaagtaaaga atagtagtat ccaacttcaa gatttgttat agtaagagca ttttgagccg
    80341 atatttcaat ttctgaaaat tgaatatttg gttttcctgt aaaactagga ggagctattc
    80401 tttctatttt atccaattgt ttttgtcttc ctcttgcaat ttttgaattt actccagctt
    80461 tatttttacg tataaatgtt tcttcttttt ctattttctt ttgttgtgct tgataccttc
    80521 taatataatc ttctcttaaa tgtgattttt gtttaaggaa ctctgaatat tttccatgat
    80581 atttcttaat catgccaaat tctatatcaa gaatacctgt agaaattttt tctaaaaagt
    80641 caaaatcgtg ggaaactaca ataaatgccc cattaaatgt atttaaataa tttgagagcc
    80701 attcaacatg ttctttatct aaaaagtttg ttggttcgtc taataataga acatcatggt
    80761 tagaaagtag taattttgct aaaattacct ttgccctttg accaccacta agctctccaa
    80821 caacacgatt cattcctatt gaatctaatc caagaccatt tgctatttta tttatttcat
    80881 tatctataga atagaaatta tttgctatta gttgttcctg ataatctgat gctttgatta
    80941 gatattgccc attttcagat atagcacttt tttgatagag tagattcatt tccttttcta
    81001 ttttataaag ttcctcaaat gcagtgtgta aatagagtga tattgttgta tctctattga
    81061 tttctgcata ttggtctaaa tggcctatat tgatattagg ttgccacttg atttctcctg
    81121 aatctggaac aacttctccc aataaaattt taattaatgt acttttacct gtaccatttt
    81181 ggccaactac acctatatgt tcacctttga ataaatcaaa gttagctttc tcatacagtg
    81241 atttatccat aaaacattgt gataaattag ttacttttaa taaactcata attcaaactc
    81301 cttttctttt attaaaaaat ttatggaaat aaaaaaagca gaggaatggc attgtaaaat
    81361 gccgacctct gcttttggaa aaacaaaagg tcatagacaa gcattgttgt ctatgaccag
    81421 aaaatctata ttatctatcg gcaaataggt aattttaaat aagcatagcc aaaaaactat
    81481 actaaaccta tatttgccga tgaagataga aagctttaag ctttcagatt aacctctaca
    81541 caacgcttca tcgtatacat ttgtgtagag ttaagttttc taacaagttt aactaattct
    81601 atataaatca taattattca catcctaatc ttagtattaa ttctagttta tctatattta
    81661 aattgtactt ttgtactttg tggattatct tatttattta aaatacgttt tattaattat
    81721 agcttaagtt atgttatcat aatacttagt tatagtcaat aagaaagtta tatttactat
    81781 tattatatta atttagtaaa tctatcatta caaagttatt tgaagactcg ctatcaataa
    81841 aattttatag tctataagaa aattaagatt atgagtttta taattttgat atagggatac
    81901 tattttcata aatattacaa catgtagaag tatatgtcta gtattgttga attttcaatg
    81961 atatattaag gttctaatat attatacaaa ataaaaacat taattcaact taaataattt
    82021 taagtattat atgtgttttg attaaagaaa actaaaattt taaggaaaaa taacatattt
    82081 gtattaaatt ttattgaaat gattaaattt atttacttaa tgtaaattat tagctaaaat
    82141 tgtattaaat aattaaggag gtgagtagct ctggatgaaa tagatttaaa aataattaat
    82201 tctctaaaaa agaatagtag ggcttcaacc tctgaaataa gcagacaaat taatttatca
    82261 gtaccagcag tctcagagcg tataaggaaa attgagcagt caaatttaat tcaaaagtac
    82321 actattcaaa ttaatcggga aaaaacaaaa tataagcttt tggcttttat atttatcaat
    82381 actgatttat ctaaaagtat gaaagaattt aagaaaacta tattggaata tgatactgta
    82441 ttagaatgtc atcatatttc aggagagtat gattacttaa taaaagtatt agcagaagat
    82501 acaagtaatc ttgaagagtt tatatcagtg actttgaagg aaattggggg gattcaaaaa
    82561 gctaatacaa ccattgtttt atctacaatt aaagaagaat ttaatacaat aaaagatatt
    82621 ttttaaataa tttactagta aagtgttaat tatattttag gaggaaaata gaatatgaaa
    82681 gttttagttt ttttagcaaa aggttttgaa actatggaat ttagtgtatt tgtagatgtt
    82741 atgggatggg ctcgtaacga ttatgggcat gatattgatg tagtgacttg tggatttaaa
    82801 aaacaagtta tgagcacatt taatatacaa gtattagttg ataagacgat agaagaagta
    82861 tgtgttgatg attatgatgc tctagctatt cctggaggat ttgaagaatt tggattttat
    82921 gatgaagcat atgattcatc ttttttaaat ctaataaggg agtttaattc aaaagaaaaa
    82981 ataatcgctt ctatttgtgt ggcagcattg ccagttggaa aaagtggtgt tttaaaaaat
    83041 cgtaaagcta ctacatatca tttgaaaaat ggtaaaagac aaagacagtt aagtgagttt
    83101 gatgtaaatg tagttaatga accaattgtt gtagataaaa atattataac ctcatattgt
    83161 ccagaaacag ctccacatgt agcttttaag cttttagaaa tgttgacatc taaagaacaa
    83221 atggatgaag ttaaattagc aatgggattt aaactgtaat ggtttataga ttttctaagt
    83281 aatcagaacc ccagtcacac atggaatcta aaattggaat gatactcttt ccaaaatcag
    83341 atagcgagta ttctactttt ggaggaacaa caggataaac cactcttata attaaaccat
    83401 ctgattctaa ttcacgtaat tgttgagtta acatcttagg cgttgcttta ggaattaatt
    83461 tttttaattc attgaacctc aatatagtgt ctttaagatg ccaaagtata agtgtcttat
    83521 atttaccacc aatcaatgca agtgttgctt ctattgggca actgtaatta tctaaacatt
    83581 ttttcatttt tgggctcctt tatttatttt aaagtatctt tttaggtact atattacaaa
    83641 aaagtgcatt attgtaatat agtaccatag tgataaaatt atagcatagg ttaaataaat
    83701 catcaactta gcctaataaa acaatagggg gtaaataaat gaatttcgtt gaacttgcaa
    83761 aaaaaagata ttcttgccgt aattatcaag atagaaaggt tgaaaaagaa aaacttgaga
    83821 aggttttaga tgttgcaaga atagctccaa ctggtggtaa tcgtcaacct caaagattga
    83881 ttgttattca ggaaaaagaa ggtataaata aactttctaa ggctgccaat atatatgatg
    83941 cgccattagc aattcttgta tgtggagata aagacaaagt ttggacaaga ccttttgatg
    84001 ggaaacaact tacagatatt gacacaagta tagttacaga ccatatgatg ttacaggcaa
    84061 ctgaacttgg attggcaagt gtttgggtgt gctattttaa tccagatata attagagaag
    84121 agtttagttt gccagataat ctagaaccaa taaatatatt attgatggga tatgaatcta
    84181 agataccaga gagcccagag agacatgaaa aaactcgtgt tcctttaagt gaaattgttt
    84241 cttatgaaac tctttaatta taaaattaag ttattcatag gataaatgta ataaagtgtt
    84301 tattataaac aaaattcaaa ataaataata tctacatata attaatttta gtgaagtata
    84361 tctagtttaa ctagaatgta tacttcacta atttatttat tcaaaatatt tatgaggatt
    84421 ttaatatatt aactaaaata tatcaattga attaactttc aaatctggaa aagcacttga
    84481 aatatagtgt tttatatcat ttaaatggtc ttgaatttgc ttttctgata ttttatcagg
    84541 tccatataca aaatacataa tatttttagt atcttttgtt atagctgttc gattatcagg
    84601 gccattaaga atactagata atataccaat attatctgat aaaaataaat catcttttgt
    84661 taaaatatgt tctttaccac ctataccata aaaatgttca cttccatttg ctaattctat
    84721 tttcaaaggt aattccattt tatcaaggtc atgacctgct gttagaagaa gatttttcaa
    84781 ttctgctaaa aacattgact caattgttac acctacatct gggatacctt ttgattttaa
    84841 tagaatagat tctaattgaa gcaatacagg gtaagttttc ttgaattttt tataatactt
    84901 tatataactt gaaacaggct ctgttttaat tttttcttta cgattgtatt ctgggtgttg
    84961 ttctataata tttttaattg tagagttttt aagaactaaa aaattttctt tattaggttt
    85021 tgaatgtgta atatttataa ccataactcc aaatttaata ttgggataaa tttctttaac
    85081 tttatgtgat atttctaaca taatatagac tcctttttat tttatatttt atgatataat
    85141 cttagcaaat taaaagtgga ttttgtagtt ccatttttat agtaatttta tagaaccact
    85201 tttggaggta ttagaatgtg gattacgatt aatagaaata gtaatatatc attatcaaga
    85261 caaatttatt gtgagataaa agatatgatt ctggatggaa gcttaaagtt tgagcaaaaa
    85321 ctgccatcat ctaggacttt atcaaaagaa ctatctgtat ctcgtaatac agttttagat
    85381 gcatataatc aattaattgc agaaggatat ttggaaacaa ggaatggata tggaacaatt
    85441 gtagctaaag gaatatcagg gcataaagtt gattttaagt taaatgatac ttttagagat
    85501 gaaaaagatt tacatactaa taaacaaaaa aattatatag attttcgttc aggagttcca
    85561 gaattaaaac tatttccacg caaagaattt tcaaagctat ataaacaaat actgtatgat
    85621 ttatcagatt cggatttcag atatggaagt acagctggtg tatgggtttt aagagtggaa
    85681 atatctaagt atttatttcg tactagagga attagatgta atcccaaaaa cattatgatt
    85741 gtttctggtt caacacaagg tttatcttta atttcacagc ttttatacaa aagtaataaa
    85801 aaggttgctg tagaagaccc tattcataaa ggcttattaa aggttatttc atctattgga
    85861 tattcagttg ttggaattaa agtagatgat aagggaatga ataccaactt aataaaatca
    85921 ataaaaggga tttcttttgt ttatactaca ccttctcatc aatacccatt aggagggatt
    85981 ttacctattc aaagaagact agagcttatt aaatgtgcaa cagagaatga ttactatata
    86041 attgaagatg attatgatag tgaatttaga tatgagggtc aaccaattag ttctctttat
    86101 gaacttagcc caaataaagt aatttacata ggttctttta gtaaaatatt atctccagct
    86161 attcgtgttg gatttatgtt attaccagac aatattttag aaagctataa aaccttaaaa
    86221 atgtactctg atgttcatac agaaacaata ttgcaacatg tattagctga atttattgga
    86281 aatggaggtt tagagaagta catatttaga atgaagaagt tgtacaatag aaaacgtaag
    86341 tacctcataa aagttttaaa cacggaattt aaaaatgaat ttaaaataaa aggtgaagca
    86401 gcagggcttc atatagttgc tcatttctat aatgttaaat ttacggaaag tttaattcat
    86461 aaaatttttg aagcaggtat aaaagtttat cctatacaac aatatgcaat tgagaatata
    86521 ttagaatatg accatgaaat tatactaggt tatgctcatc ttagttttga tgaaattgaa
    86581 aaaggtgtaa aaatactcag caaaataata catatgaatc aaggtacata gataaaacat
    86641 atagcaatct acatataata gatgtaatta agtttaaact ttaaaagaaa tatatattaa
    86701 tatttttgta taaaaatacc tgatattttt ttatcgatac tttattgcta tacactaaat
    86761 taagatagca atttatatta agaaaaaact ttttgcatag gttataatac ttaaaaggag
    86821 aggatagagt tgaaaacaaa tacagatgtt attatggaga taattgatta tatagaagaa
    86881 aatcttacag aaaatctaaa tttagataaa atagcttata ttgctaatta ttcaaaatac
    86941 catttacaca gaatgtttac aaatgttgta ggatgtacta tccatcaata catacagagg
    87001 aggagactta ctgaagcagc tagacaactt atatttacag acaaatatat tatagacata
    87061 gctattacag taggatattc tacacatcaa tcttttacac tagcatttag aaatttatat
    87121 aaagaatctc cacaagtata tagaaataaa gatgaatttt atcctattca acagagattt
    87181 gttttaaatc taaatcatca aattcaaagc gaaaaatgta tttcttatgc aaatatgaat
    87241 tataaacaaa gaagagatga aatcaattct tatctgaact ttaatattaa ttgtgaggtg
    87301 aaagtagcat gagtttagtg ccacatgtaa ttgaacaaac aggtcaaggg gaaaggtctt
    87361 atgatatata ttcaaaatta ttaaaagata gaattatatt tataggagaa gaaattaatg
    87421 atgctattgc aagtttagta gtagctcaat tactgttttt agaatcagaa gacccagatt
    87481 cagatataat tatatatata aatagccctg gtggctctgc tacatcagga tttgctatat
    87541 atgatactat gaattatgtc agatgtgatg tatcgactat ttgtattggt atggctgcaa
    87601 gcatgagtgc ttttttactt gcaggaggaa cacatggaaa aagatgtgct ttacccaatt
    87661 cagagattat gattcatcag ccaatgggag gagctaaagg tcaagcaact gatgtaaaaa
    87721 tagctgtaga taatatattg aaaataaaag aacgattaga taaaattctt tctgaaaata
    87781 caggtaaatc aatagaagat attaggagag atacagatag agataatttt atgactgcac
    87841 tagaggctaa agagtacggg ctgattgatt atataatgaa taggaatgaa taaagtatca
    87901 aagtatcata ttaaaatgtc tgttttacaa gtatatatat aatattaaat aagagtgtct
    87961 caaaatgaac tttttaattt atgggacact cttttttgta tgaaattaaa tatggtttta
    88021 tcatttcaac aattattacc aaatttcaag tttaataaat tctactataa aaaatacata
    88081 aaagataagg ttctttagta actttctgtc gtcaaaagtt tttataaatt taataacaaa
    88141 acaagagaaa ataccatata ttacttatgt ctgcaatata tagaagacaa aggagttgta
    88201 cattatgatt acaataagtt tgtgcatgat tgttaagaat gaggaagaag ttataggcag
    88261 atgcttagaa tgtgtaaagg atatagtaga cgaaattata attgtagata ccggttctac
    88321 agattccaca aaagaaatag tctcaaaata tacagacatg gtgtacgatt tcgaatggat
    88381 agatgatttc tctgctgcaa gaaatttttc attttctaaa gcttctaaag actatactat
    88441 gtggcttgat gcagatgaca taattttaga agctgataga gaaaaattat taaagattaa
    88501 agaaagcttg gatacttcca ttgatattgt tatggcaaaa tacaatgtat cctttgatga
    88561 aaatggtaac ccaactctct cgtattatag agaacgattg tttaaaagaa gttctaatca
    88621 taaatgggtt gaccctattc atgaagtagt acctcttttt ggagaggtat tttattctga
    88681 cattgcaata tctcataaaa agcttcatag acaggaccct ctaagaaatc ttagaatttt
    88741 tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga caccaattct attattctag
    88801 agagttgtac tataatgcta ggtataaaga agcaatagaa ggttttacta agtttttaga
    88861 ctcatcaaga ggatggattg aggattgtat cagtgcttgc agagatttag ccacatgtta
    88921 ttacttaatt aatgatgaaa aaagtgcatt atattcattg tttagaagtt tcgaatttga
    88981 tgagccaaga gcagaaattt gttgtgacat aggaaagcat atgtttgata gacaaaaata
    89041 taaagaagcc attttttggt ataaggtagc attaacaaga gataagaatg atactaatgg
    89101 aggttttaag tcaaatgatt gttatggata tataccatct atacaattaa gtgtctgtta
    89161 tgacaggtta ggagaatcag ataaagctat ttattatcat gaaaaaacaa aagaaatcaa
    89221 acctaacgat agtgctgttt tgcacaatga aaattatttt agtaaattta aaaatagttg
    89281 ataggtcaca atcatactaa atttgacata ttatatatag agcttaaaag ctcaaatata
    89341 tcaggaggtg cttttaataa tgagtagaaa taaatatttt ggaccatttg atgataatga
    89401 ttacaacaat ggctatgata aatatgatga ttgtaacaat ggtcgtgatg attataatag
    89461 ctgtgattgc catcattgct gtccaccatc atgtgtaggt ccaacaggcc caatgggtcc
    89521 aagaggtaga accggcccaa caggtccaac gggtccaaca ggtccaggag taggggcaac
    89581 aggcccaaca ggaccaaccg gtccgactgg cccaacagga aatacaggga atacaggagc
    89641 aacaggatta agaggtccaa caggagcaac aggggcaaca ggcccaacag gagcgacagg
    89701 agctataggg tttggagtaa caggtccaac aggcccaaca ggagcgacag gagcaacagg
    89761 agcagatgga gtaacaggtc caacaggtcc aacgggagca acaggagcag atggaataac
    89821 aggtccaaca ggagcaacag gggcaacagg atttggagta acaggtccaa caggcccaac
    89881 aggagcaaca ggagtaggag taacaggagc aacaggatta ataggtccaa caggagcaac
    89941 aggaacacct ggagcaacag gtccaacagg ggcaatagga gcaacaggaa taggaataac
    90001 aggtccaaca ggagcaacag gagcaacagg ggcagatgga gcaacaggag taacaggccc
    90061 aacaggccca acaggggcaa caggagcaga tggagtaaca ggcccaacag gagcaacagg
    90121 agcaacagga ataggaataa caggcccaac aggtccaaca ggagcaacag gaataggaat
    90181 aacaggggca acaggattaa taggtccaac aggggcaaca ggaacacctg gagcaacagg
    90241 tccaacagga gcaacaggcc caacaggagt aggagtaaca ggagcaacag gagcaacagg
    90301 ggcaacagga gcagacggag caacaggagt aacaggtcca acaggagcaa caggggcaac
    90361 aggagcaaat ggattagtag gcccaacagg agcaacagga gcagcaggaa cacctggagc
    90421 aacaggtcca acaggagcaa caggcccaac aggagtagga ataacaggag caacaggggc
    90481 aacaggagcg acaggtccaa caggagcaga tggagcaaca ggtccaacag gagcaacagg
    90541 aaatacagga gcagatggag tagcaggtcc aacaggagca acaggagcaa caggaaatac
    90601 aggagcagat ggagcaacag gtccaacagg agcaacagga aatacaggag cagatggagc
    90661 aacaggtcca acaggagcaa caggagcaac aggagtggca ggagcaacag gagcaacagg
    90721 tccaacagga gcaacaggaa atacaggagc agatggagca acaggtccaa caggagcaac
    90781 aggagcaaca ggggcagatg gagcaacagg tccaacagga gcaacagggg caacaggagt
    90841 tacaggagca acaggcccaa caggagcaac aggagcaaca ggagcaacag gtgctagtgc
    90901 aataatacct tttgcatcag gtataccact atcacttaca actatagctg gaggattagt
    90961 aggtacacct ggatttgttg gatttggtag ttcagctcca ggattaagta tagttggtgg
    91021 agtaatagac cttacaaacg cagcaggaac attgactaac tttgcatttt caatgccaag
    91081 agatggaaca ataacatcta tttcagcata cttcagtaca acagcagcac tttcacttgt
    91141 tggttcaaca attacaatta cagcaacact ttaccaatct actgcaccaa ataactcatt
    91201 tacagctgta ccaggagcga cagttacact agctccacca cttacaggta tattatcagt
    91261 tggttcaatt tctagtggaa ttgtaacagg attaaatata gcagcaacag cgcaaactcg
    91321 attcttacta gtatttactg caacagcttc aggtctttca ttagttaata ctgtagcagg
    91381 atatgcaagt gcaggaattg caataaatta gattagataa gaaaaggctt ctaatatcgt
    91441 attgatatta ggagcttttt taagaatgat tgatgaacat agttttaata ttaattagtg
    91501 cgcatattat aaaatactat tatataatat aaaataataa cattaaattg gaaatataga
    91561 attatctagg ggtgatacta tgaaaaataa gattgaaata tcaatgaaca actttgtaaa
    91621 aaattatcat aaatcaaata atataactac aaagtggcaa gaacttttgt ttgcttatgc
    91681 atctgctgaa gatgatatgt ttcagaagct aaaaagtgtt gtaggaacat ctcatgctat
    91741 gccaaaagat tttctggaaa atggaaaaac tataattaca tattttatac cttttgatga
    91801 aagtgtaaat aatagtaata ttggagggtt tgaatgttct gaaatatggg caagagcgta
    91861 catagaaact aatatgttga tttcaaattt aaatgatttc ttatgtgaag aactagagaa
    91921 gataggggct aattcatatg ctattcctgg gtcgtataat tttgacagtg aaaaattggt
    91981 aagtgactgg tcacaaagac atgtagcatt catagcaggt cttggaactt ttggtttaaa
    92041 taatatgctt attacagaaa aagggtgttg tggaagaata ggaagcataa tcacaagttt
    92101 agaattagag ccaactaaaa gaccagaaaa tgaatactgc ttgtataaac ataataatag
    92161 ttgcaaaaaa tgtgtcaata aatgcgtgaa agaagcttta aaagaagatg gatttaatag
    92221 gaataagtgt tatgaaatgt gtatgataaa tgaaaaaaaa tacacttata taggtggatg
    92281 tgatatttgt ggaaagtgta tagttggagt tccttgttca gtaataaacc catcaagata
    92341 atggacttaa gttgactaat aattttaaag atagtttata atgaaaaaat tttagttatc
    92401 aaaataaata taatataaaa actaaataaa tgtgtttaaa aaatggataa ataatttgtg
    92461 ttgattgaca accagttata ctgttgatag acttatatgt atcgctgtga tatataagga
    92521 gggttgagat tttatgtttg atttaatctt tatttttgca tagcaagggc tattgcaact
    92581 atgaaattta atatataaat agccctttgc tattcctaat gaaaaattac tatttaatag
    92641 gagagcaaaa tgagctataa aaaagcaata catatattac cagaagaatt acttgaatta
    92701 atacaggaat atgtagatgg tgaatgtatc tatattccta gaaaatctaa taataaaaaa
    92761 gaatggggaa gtaatacacc tactcgtgaa gagttgtcaa ttagaaatat gcagatttac
    92821 gaagactatc aagttggata tgatttgcaa tatttatcgg aaaagtatta tttatctttg
    92881 aaaagtatcc aaagaattgt attacaagaa aaaagaaaag atatctaagg atgagccagt
    92941 atagttttat aattatactg gctcattttc ttttgataaa agtgtttgag gtatagtttc
    93001 ttttaagtgg atgatataat ttaagtaatt tcaataactt atacagtagc taacaaagct
    93061 ggttgtaaag tatctaataa gaagtagtag ctttttatat gtttgaaatt aaaatttatt
    93121 aaaattagga ggtcttgttt tgaaaaaaat acttgattat attccacctt ttcataactt
    93181 tctattaatt tagtaatctt atagaaagga aattgcattt atgaaaaaaa ttacttggga
    93241 ggtggaatat atctcccagt tacctgtaat taggtattgt aaaaaatgtg gaaaaaagac
    93301 tgaatatatt tgttcaggtt tatttagggt taatgctcaa cataaatatt tggatatatg
    93361 gttaatatac aagtgttcaa attgtgattc aacatggaac tcaactatat attctcgtgt
    93421 taatccaaaa agcttaaatc cagaaatact agaacaattc catacaaatg attataatct
    93481 tgttaagcaa tatgctatga atatggaact tttacataga aatggagcag aagttggtat
    93541 tccagagtat aaaatagtag gacaagaaat taatattaat gaccttacac aagttcatat
    93601 tattagtaaa tatccatgtc agttaaaggt atcaacatta ttacgtgaga aactaggttt
    93661 atctaggaag gtatttgacg aaatgttgga ttgtgggatt attagaagta actcaggagt
    93721 tgatttaaag aaaggtaaat taaattatga aattatgtta gatataggca aggagaagca
    93781 aaagtcttca aattgattag aataactaac ttaatatatt ttcaggaaca tcatttaaaa
    93841 aaatagtgac tttaaaaaat ttaaagccac tattttttaa tatgttttat atgagtattt
    93901 aaaataaagt gtgtattctt caaatttata gcgataatta aaacattact ataaaaagga
    93961 aggtgcatac tttttattta acaaaaacta gatattaaaa acttttcgag gaatatttta
    94021 ttaaaatccc taaatatatt taatatatat taaaaaattg tattataatg aaaatcaaaa
    94081 tagattgcaa atccaaaaag taatacttaa tgtccaaaaa gctaaaatta aaatctaaat
    94141 agctatttac tacagaagta tgttaaaata tgtaaaatga atagaaattt atatattttt
    94201 attaaaaata tataagaaag gatgtttaag aattgtgatt aatataggaa tatgtgatga
    94261 tgaactacat tatcgaataa aaataaaaga tatattaagt gaaatattaa gttcatatcc
    94321 tataaattat aacatatatg aattttcagc aggagaagag ttactaaata actatcctaa
    94381 agatttagac atattaatta tggacataca aatgaaaaca ataaatggta tggatactgc
    94441 taggaagata agagagtttg atcataagtt agaaataata tttgttactt catttgtaga
    94501 gtttatgcaa gaaggttatg aggtaaaagc atacagatat atactaaagc caataaataa
    94561 agaaaaaatt tctaaaagtg tattaccttg tataaatgag atgatgaaaa agagaaataa
    94621 ttacttaact ataaatgtaa aaaattatgt agaccgaatt aaaatagatt ctattactta
    94681 tatagaaaca gatagaccga atatattgat ttatacacat gatgatatgt atattactaa
    94741 aatgagtata tctaaaatag aaaagatatt aaatgaatat ggatttttta gatgtcataa
    94801 tagctatata gttaacttaa aattagtaga atcaatgagt ggaagtactg ttatagtaga
    94861 tggaaaaagt attccaataa gtaaatatag ggttaaaggc ttgaagcttg cgataactaa
    94921 tatattaggg gacatagtat gctaacaaat ttaagtgtag gattttggaa tattattaat
    94981 ttagtttcag gtactataga ttggattata ttttttttag taatccactt agttggaaag
    95041 aggaaaatat ctaatataag atatatattt acaacaataa ttataattat atttatggca
    95101 tacataaatg tagttaaagt gtttccaaat attaagattc ttctatgtat ggtaatagga
    95161 atatcatttt atataacatc atatgaagat aaaatctata aaagtataat agttagttta
    95221 ttgttttggc taggattaat ggttgctgaa ggttctgcag taggtctagt agtatttata
    95281 aataaattaa ataatattaa cataacacaa catgaaaact tatttagaat acaagctatt
    95341 attatgtcaa aggtattttt atttataggt tttatattgt ttaagtattt taaattatct
    95401 ttagatttta aacctaaaga tatgatacta ataggaatac ctatattatc taatatagtt
    95461 agcttattac ttatatttga atataattta agagtcaata caattactac agaaaactta
    95521 tttatattta tatttgcaat tttattgatt ttatcatcaa gtataatact tttaatagta
    95581 atagggaaaa ttgtacaaga cgataaatta aatctagagt atgaacttat aaatgaaaga
    95641 ataaaaacaa actacaggaa ttatgaaaat ataaacgaag ttcatgatag attaaaatat
    95701 gtttatcatg acttgaaaaa ccatatgatt tgtataaaaa attatgacac taaagaagaa
    95761 attatatcat atataaataa cttagagttt cagataagtg aatttgaaca ttttaaaaat
    95821 actggaaata aaacattaga tttaatttta gcagaaaaaa taagtatttg taaaaagtat
    95881 gatattcaaa ttgaagataa cataaatata tctaaattaa actttattaa aaataatgat
    95941 atatgttcaa tatttgcaaa ttcactagat aatgcaattg aagcatgtat ggatataaat
    96001 aatgagatag aaaaaagaat agaagttaaa gctacatata taaataaatt tgcaataata
    96061 aaatttataa atactaaggt aaatgatatt aaacttattg ataaacgtat tcaaactagt
    96121 aaggatgata ataagataca tggaatagga cttgctagta ttaaatatat agtaaataaa
    96181 tatggtggtg aaacaatagt taattactca gataatgagt ttatattaaa aataatgata
    96241 ccaataaaaa gttaggagag tacaaaatga aaaaaatagc gttaaactta cttaaaaaca
    96301 tctcagcatt aagttttggg atagctgtat tatcagcaaa cagtgcaagt tcttgggtag
    96361 cacatcaggc aaaagagcca caagctttac aaaaactaaa aaaataacat taataaaagg
    96421 acctcttctt attaaaggag aggttttaca tgttgaggag gaaatatgtt taaaagatta
    96481 tcatataaat ttgccaatat attagttaat aatgagattg tagaaagtga agattttgaa
    96541 atatatagat atggatttga aactttgatt tatttcatag ttaatatttt ggtagcatta
    96601 tttataggaa ttatttttga tagatttata catacagtta tatttttaag ttgttattgc
    96661 actttaagac agttcacagg aggttatcat gctaggaatt ataaagagtg tacattaaca
    96721 tttgcagtta tttatttgat aactattttt tcagctaata atatagatat aaatataagt
    96781 atttattagt attattaatg attattagta tactaacaat ttacaagtta gctcctttag
    96841 aacatagaaa caaacctctt agtgaaagtg agaaaaagca ttatagaaag actgtgcaaa
    96901 aaatactatt cgtaataata tgtttaatta ttttatgtaa aatacttaat atttttcagc
    96961 aatatatgat ttatgcaata atttctatat attggattgc tatattaata tatattggaa
    97021 tgaaggttaa tagtgatcag taagattata aaacattttc taaattaatt aacttcacta
    97081 attaatatta actttaaaaa aataatgtca aggtaagtat ttgaacttat taaatatagt
    97141 tgaatctatt gtgggttgta tcaaaggaca aatagattca cttaataacc agtttgtaac
    97201 agagtattga attgtaaaag ttaatacgat agtatgaaaa tgtaatttga aattacattt
    97261 ttataaaaat tatattaact aaggggaggg atacatacaa tgaatttgaa gaaaaaaatt
    97321 aaaaagttag gagttacatt tgcaatagga actataattg tatcaagttt agggacatct
    97381 gtatttgcag atgaggttag cacatatgct ttaatatcta aagctttttc aggtaaaaac
    97441 aatattatat ctagttcagt atacacttct ggaagtagaa cctttaaaac gacagattct
    97501 caagggggat tttcttgtag gctaacagaa agattctcag gatctagccc agatagaatt
    97561 gtaaaaagac atgaagcaaa tgcttcttca aaagttagtt tatcaggagg aacatactat
    97621 ataaggtatg aaggttcaac gaataatgct aaaacatatg caactttaaa ttagtattaa
    97681 aagcatccta taattatttt tataggatgt ttttgtaatt gttataatca aaagactggc
    97741 taggaggggc ataaatatga gtagtattaa ggttatatca gactttataa ccaaaataat
    97801 tagaaaaaat attttatttg caataacagt tattattata aattaccatc tcatacaacg
    97861 atggacacat atatccaata gaggaatttc atttaatgat tacttatcct tgagtgtata
    97921 tacttgtatg acaattagta ttttaaccat gttaaatgta attttttata tgagaaaaag
    97981 ttatgatact gtaaaaatat tatgtagaaa taattttgat tattttttag gtatttctgt
    98041 tgctgtaact agaaacaact ttatattttg ttgcatacca attatatatg ctatagcata
    98101 ctcaagttta ataagaacat tgtcaattgt aacgattaaa tcaactatat ttatattgat
    98161 atttgaatgg tttttaccac ttactatttt atctatagtg atatcattta tttcagttat
    98221 tataaataat ttaatactaa agattacctt atcatcagta tttttatatt taacatcaac
    98281 taatatgttg gatagagtta ttgatattaa agaaccattt tttaaagcat tatttaaatc
    98341 acttaacata tttgaagatc aatcatatgc ttactattct gattttgtag ggaaagttaa
    98401 taattcagca tatgttatag ataagattat acctacacta tttgcaatta ctttaataat
    98461 agtagcatat ctgatattaa atgatattag aaagaaacag attaaatcat taatagttat
    98521 agtagtgtac agtattttat ttattggatt aaattatata tcaatagata tgtcaacata
    98581 tagagatgag gagcgtagtt atggagagta tttaaagaaa tctaatatta gtgatattaa
    98641 aataaaatcg catgatatga agattgactt taaaagcaag tcaaatataa aagatagaat
    98701 tgttttatta aacacatcaa atgaagagtt aacaaatatt aatatgattt tagatgaaat
    98761 atttgatata aaatatataa aagtaaattc ggaaaatata aaatttaaca taaaaaagga
    98821 tttaattgaa gttaatttaa aggaaagttt agagcctaat gacgaggtga cattagatat
    98881 agagtatgaa ggctatatta atgtgttgac atcttggaat caaaataaat atatagctac
    98941 taaagatgat attatgcttc caccaggttc tttagcgtgg tacccaaaag taaatcaatc
    99001 aagttatatg gcttataaaa taaatttaag ttctaaaagt aatgtctatt caaatttacc
    99061 tttactgaat aagactagta attttttaaa aaaagattat atatttgaag gaaagtcaaa
    99121 tgatattgca atatatgcag gtgagtttaa agaagaggta aaagatgata taaaaataat
    99181 ctatccatca gatagtaatg taaatatgga tatagaggaa aaaattaaat ttttaattga
    99241 aacaagaaat gaaatattag gtgatagcaa agcatctgta gatgctaaaa aatatgtaga
    99301 tgaagtagat aatgttatgg aatcaataaa aagaaaaaaa attaatatga tattagttgc
    99361 tgacttacct agcaatgaat ttttatatga agactcaaag ggagagaaaa ttagcataac
    99421 tatttatcat tggctttcag gaaatatcct tataatagat aaataataaa ggaggttaat
    99481 atgataaaca agttagaaat aagaaatcta aattttaatt acaaaaataa aaaagctttg
    99541 gataatataa atttaaccct aacagatgga gtagttgcat tattaggtcc taatggtgct
    99601 ggaaaatcaa cattaatgag gttattagtg actctttatg aaacttcaat tggagagata
    99661 gaattaaata atataaagta tagcaaaaac aatgagaaaa tcaaggcaaa tgtaggatat
    99721 gtacctcaag attttgatat gtataataat ataaatgggc aagaatactt agaatttgta
    99781 gctaaaatga gaggtgtatc taagaatgat ttaaagaaac atatacaaaa agtagtatca
    99841 aaagttaatc tggacaagtt tataaataaa aaaataggta catactcagg tggtgtaaag
    99901 agacgactag gtatagcaca ggctttaata ggtgattcta agttaatagt tatggacgaa
    99961 cctacagttg gtcttgatcc agagcaaagg aatgaattta gaagattgtt accagaaata
   100021 agtaaaaatt ctatagtatt aatttcaact cacatagtag aagacataca atttaactgt
   100081 gacaaattaa ttatattaaa tcaaggcaaa atattatatg atggtacaat taataagttt
   100141 attgatatgg ttgaggtgta ttcggtagta gtttctaatg atgaatttga atatttagaa
   100201 cgtaatattg atattattga ttataggaga gttaaagatg gtgtgcaagt aaagtatata
   100261 ggtaaaaata cagagaaact taaagatagc ttaaggatag atacaacttt acaagatgct
   100321 tatataaact ttttacataa ggagttaaaa taaatgtgta tattggagtt aataaaattt
   100381 gagcttaaaa aaatagagct taaacatgtg ttaatatttg taattatttc tatattatgc
   100441 atatttatta caggcatatt agattataat gagttaatat tttataatga tgtacctata
   100501 atggatacat attctatagc aggatggtta ggaattataa agatatcaga tgtttttata
   100561 ccaataatgt cagcaatcac aataatttat gtgttttcaa aagattattc gggtaatata
   100621 aatgagatac ttactttata taataaaaaa aaatataatc aatttatagt atttagatgg
   100681 tgtgttatat tagctatata tactgtatta ttattaattt caattacaat agtatataca
   100741 tttacgtcag tgaaaggttt taacatagac aaaatagatt ttgtttatag tgtaaagcca
   100801 atagatgtgt ttttaaagac gtttcctaca ttattatggt atacaacatt tccactaatg
   100861 atattaacat taacaaaaaa tagatttaca acaatagcaa tattaattac atatacattt
   100921 gctgatatat tttttatttt acatatgtac ccatttgtta gtatgattaa tgtaaatgga
   100981 ttttatattc aaaagatgtt ttatatgcct aacaatatag atgttagatt taatgaatta
   101041 aagaattatt tctttataaa tagaggttct ctagtagtta tttctataat aatagttttt
   101101 tatatagcaa gaaaatcaac ggttttaaaa aagagtaaat agtttaatat attttttata
   101161 ttgttatatt tatatagaat agtgacttta aaaaatttaa agccactatt ttttaatatg
   101221 ttttatctat ataactatta tttttctagg ttttttaata tttcgtaata gtgtgttttg
   101281 gctatttcac ttgactcgcc ttttttacaa aaagataatt tggatagttt tggaactaca
   101341 ctttcaacga caattttatt acctatcatt ttagattcaa gtatcttcaa tgcttctaaa
   101401 aatctattat tcttttttgc acaattataa aatgataaaa cgtagacata aaggaatagg
   101461 ttataattac ggaaaggata ttcaacttgc ataaataagg ttccaattcc atagtgacaa
   101521 gggccgattg gttttttaat agtccaatgg tcaagtagaa aatctacagc tttgtcaagt
   101581 tttgattctt tatttagata attactaaaa cggaatgcat ttaatatatt aagagttggt
   101641 agtggatttg agtattcagt ttctggacct cgaccaaaac taaatttatt gcaacgccac
   101701 ccaccatcag tatactgtgt atctaaaaaa tgttgaaacg ttttttgcat tctaatgtca
   101761 gatgcatatc ccatatgaca gagtacgtta gcagcatttg cagtatgaca agggtaaata
   101821 cctccatttg gatatagttt gaaacgtcca tcttctcgcc atgtgctaaa tatcaaaaac
   101881 tttattataa tattatttat atttataatt gatttatttt atttaaaatt gcatttatag
   101941 ctgtatcagg aacaactgct aaatcttctg cattgaagta ataaactata ttagaatcaa
   102001 ataatatatt tgaatttgga ggaaattcat catctccaaa ccaaagtata aataccatag
   102061 atgtattacc catatatgta aacctataag ctaaatctcc catgtttact ttttcagctc
   102121 caattttctc cataactaac ttatatttgt ctatttgaga tgtaaaaact tctgaaagcc
   102181 tatatagagt cctgtttaaa aaatttggat aataaacatt gcctcctggt atttctttaa
   102241 aagttacata tttattagtt ctaggaatac ctttagcatt gattaaatat ctaactatca
   102301 agattttaag ctcaaagtta ttgcaaaggc taccattact attccaaact tctccatttg
   102361 gatagtctat aatatattgt ctatcaaaaa aattgagtgt gaatctattt aaatcattat
   102421 tgaaaggtat attacttaaa ttactaattg tatgagggtc taattctttg agtttttttc
   102481 gaacatagtc aaatggaaca ttataattat tttttgttat cttttcatcc ataatttttt
   102541 cctccaattt aaacaattaa aagtatattt ataaaaagtt ttcttattat taattttatt
   102601 atatattcaa aatattacaa taataaattg ttaaataaat ttttatattt aagtaaatac
   102661 gtgtaaaatg aatataaagt ttttgaaata taaagttttt gtatctaaaa tattttattt
   102721 gtaaaatagt aattaattat tgtaagctat aatataagag tattatggtt aatgtgaatt
   102781 ttaaagatat tattaaatat ataaattaga atgctattag atggataaat ggaggagtta
   102841 ttatgtctaa gatattagta gtagatgatg agttagatat gttaaagttg attgaaaatg
   102901 tgttgaaaag agatggacat gaagtaaagg taattagtga tgtagaaaag gctatgaaca
   102961 tggattttaa ttattttaat ctaataatat tagatgttat gatgcctaag atggatggtt
   103021 ttgaagtatg taaaactata agaaataagg ttgattgtcc aatactgttt ttgacagcaa
   103081 aaaatatgga aagtgatatt atgtacggtc ttggtatagg tgcagatgac tatataacca
   103141 aaccttttgg tgtaggagaa ttaagggcta ggataactgc tcatttaagg cgagaaagta
   103201 gagaaaaaaa gaatttatta actatctcaa atgttcaatt taacttttta ggcaaagaag
   103261 tttcaattga tggtaaaaaa attaatttta ctaaaagtga atatctaata tgtgaatttc
   103321 tagcaaaaaa taagggacaa gtattttcaa aagaaagaat atatgagtcg gtatatggtt
   103381 ttgatggaga aagtgatata agtactatca cagagcatat aaaaaatata cgaagcaaat
   103441 taaaacaata tgatatttat acaatagaaa ctgtatgggg gatagggtat aaatgggtgt
   103501 aataagtata agtgttgttt agtgaaaata ttatatatat ctacaa
//
LOCUS       DAENCV010000002        68505 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAENCV010000002 DAENCV010000000
VERSION     DAENCV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.002"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            68..1282
                     /locus_tag="KQD82_000088"
     CDS             68..1282
                     /locus_tag="KQD82_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG4378595.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1459..3372
                     /locus_tag="KQD82_000089"
     CDS             1459..3372
                     /locus_tag="KQD82_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4378596.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGIFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            3643..4617
                     /locus_tag="KQD82_000090"
     CDS             3643..4617
                     /locus_tag="KQD82_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG4378597.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            4638..4913
                     /locus_tag="KQD82_000091"
     CDS             4638..4913
                     /locus_tag="KQD82_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG4378598.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            4926..6089
                     /locus_tag="KQD82_000092"
     CDS             4926..6089
                     /locus_tag="KQD82_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG4378599.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            6427..8706
                     /locus_tag="KQD82_000093"
     CDS             6427..8706
                     /locus_tag="KQD82_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4378600.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(8801..10141)
                     /locus_tag="KQD82_000094"
     CDS             complement(8801..10141)
                     /locus_tag="KQD82_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4378601.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFYLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(10153..10935)
                     /locus_tag="KQD82_000095"
     CDS             complement(10153..10935)
                     /locus_tag="KQD82_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378602.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(10950..11972)
                     /locus_tag="KQD82_000096"
     CDS             complement(10950..11972)
                     /locus_tag="KQD82_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG4378603.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGKGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(11978..12991)
                     /locus_tag="KQD82_000097"
     CDS             complement(11978..12991)
                     /locus_tag="KQD82_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG4378604.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(13119..14057)
                     /locus_tag="KQD82_000098"
     CDS             complement(13119..14057)
                     /locus_tag="KQD82_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4378605.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAVPFFSQSYNPIGKELLLESVKNELTK"
     gene            14327..14791
                     /locus_tag="KQD82_000099"
     CDS             14327..14791
                     /locus_tag="KQD82_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378606.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(14903..15220)
                     /locus_tag="KQD82_000100"
     CDS             complement(14903..15220)
                     /locus_tag="KQD82_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4378607.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(15233..15964)
                     /gene="chbG"
                     /locus_tag="KQD82_000101"
     CDS             complement(15233..15964)
                     /gene="chbG"
                     /locus_tag="KQD82_000101"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG4378608.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(15990..17300)
                     /locus_tag="KQD82_000102"
     CDS             complement(15990..17300)
                     /locus_tag="KQD82_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG4378609.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCTNPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(17377..18690)
                     /locus_tag="KQD82_000103"
     CDS             complement(17377..18690)
                     /locus_tag="KQD82_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4378610.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(18712..19023)
                     /locus_tag="KQD82_000104"
     CDS             complement(18712..19023)
                     /locus_tag="KQD82_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4378611.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(19223..19927)
                     /locus_tag="KQD82_000105"
     CDS             complement(19223..19927)
                     /locus_tag="KQD82_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4378612.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(20018..21379)
                     /locus_tag="KQD82_000106"
     CDS             complement(20018..21379)
                     /locus_tag="KQD82_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4378613.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            21878..23032
                     /locus_tag="KQD82_000107"
     CDS             21878..23032
                     /locus_tag="KQD82_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4378614.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(23114..24832)
                     /locus_tag="KQD82_000108"
     CDS             complement(23114..24832)
                     /locus_tag="KQD82_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG4378615.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLTSESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(24837..25826)
                     /locus_tag="KQD82_000109"
     CDS             complement(24837..25826)
                     /locus_tag="KQD82_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4378616.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(25922..26971)
                     /locus_tag="KQD82_000110"
     CDS             complement(25922..26971)
                     /locus_tag="KQD82_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4378617.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(27248..27916)
                     /locus_tag="KQD82_000111"
     CDS             complement(27248..27916)
                     /locus_tag="KQD82_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG4378618.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(27922..29295)
                     /locus_tag="KQD82_000112"
     CDS             complement(27922..29295)
                     /locus_tag="KQD82_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG4378619.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(29292..31070)
                     /locus_tag="KQD82_000113"
     CDS             complement(29292..31070)
                     /locus_tag="KQD82_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG4378620.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(31187..31510)
                     /locus_tag="KQD82_000114"
     CDS             complement(31187..31510)
                     /locus_tag="KQD82_000114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG4378621.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(31497..32474)
                     /locus_tag="KQD82_000115"
     CDS             complement(31497..32474)
                     /locus_tag="KQD82_000115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG4378622.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYFDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(32489..33052)
                     /locus_tag="KQD82_000116"
     CDS             complement(32489..33052)
                     /locus_tag="KQD82_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG4378623.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(33104..33595)
                     /locus_tag="KQD82_000117"
     CDS             complement(33104..33595)
                     /locus_tag="KQD82_000117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG4378624.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(33598..35523)
                     /locus_tag="KQD82_000118"
     CDS             complement(33598..35523)
                     /locus_tag="KQD82_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG4378625.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(35525..35836)
                     /locus_tag="KQD82_000119"
     CDS             complement(35525..35836)
                     /locus_tag="KQD82_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378626.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(36466..36741)
                     /locus_tag="KQD82_000120"
     CDS             complement(36466..36741)
                     /locus_tag="KQD82_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG4378627.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(36856..38265)
                     /locus_tag="KQD82_000121"
     CDS             complement(36856..38265)
                     /locus_tag="KQD82_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG4378628.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(38636..39298)
                     /locus_tag="KQD82_000122"
     CDS             complement(38636..39298)
                     /locus_tag="KQD82_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG4378629.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(39552..41771)
                     /locus_tag="KQD82_000123"
     CDS             complement(39552..41771)
                     /locus_tag="KQD82_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4378630.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(42047..44701)
                     /gene="adhE"
                     /locus_tag="KQD82_000124"
                     /gene_synonym="adhC"
     CDS             complement(42047..44701)
                     /gene="adhE"
                     /locus_tag="KQD82_000124"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG4378631.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            45205..45405
                     /locus_tag="KQD82_000125"
     CDS             45205..45405
                     /locus_tag="KQD82_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378632.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(45612..46202)
                     /locus_tag="KQD82_000126"
     CDS             complement(45612..46202)
                     /locus_tag="KQD82_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG4378633.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(46205..47080)
                     /gene="dpsA"
                     /locus_tag="KQD82_000127"
     CDS             complement(46205..47080)
                     /gene="dpsA"
                     /locus_tag="KQD82_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG4378634.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYTLDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(47156..48547)
                     /locus_tag="KQD82_000128"
     CDS             complement(47156..48547)
                     /locus_tag="KQD82_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG4378635.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENLLSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(48551..49687)
                     /locus_tag="KQD82_000129"
     CDS             complement(48551..49687)
                     /locus_tag="KQD82_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG4378636.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEETKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(49719..50264)
                     /locus_tag="KQD82_000130"
     CDS             complement(49719..50264)
                     /locus_tag="KQD82_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG4378637.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            50588..51367
                     /locus_tag="KQD82_000131"
     CDS             50588..51367
                     /locus_tag="KQD82_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378638.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            complement(51822..52250)
                     /locus_tag="KQD82_000132"
     CDS             complement(51822..52250)
                     /locus_tag="KQD82_000132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG4378639.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   52439..53053
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=52505..52533
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(53237..53503)
                     /gene="cas2"
                     /locus_tag="KQD82_000133"
     CDS             complement(53237..53503)
                     /gene="cas2"
                     /locus_tag="KQD82_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG4378640.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     gene            complement(53506..54486)
                     /gene="cas1b"
                     /locus_tag="KQD82_000134"
     CDS             complement(53506..54486)
                     /gene="cas1b"
                     /locus_tag="KQD82_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG4378641.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            complement(54489..55040)
                     /gene="cas4"
                     /locus_tag="KQD82_000135"
                     /pseudo
     CDS             complement(54489..55040)
                     /gene="cas4"
                     /locus_tag="KQD82_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            complement(55055..57376)
                     /locus_tag="KQD82_000136"
     CDS             complement(55055..57376)
                     /locus_tag="KQD82_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG4378642.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            complement(57387..58088)
                     /gene="cas5b"
                     /locus_tag="KQD82_000137"
     CDS             complement(57387..58088)
                     /gene="cas5b"
                     /locus_tag="KQD82_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG4378643.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKVLN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            complement(58090..59004)
                     /gene="cas7i"
                     /locus_tag="KQD82_000138"
     CDS             complement(58090..59004)
                     /gene="cas7i"
                     /locus_tag="KQD82_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG4378644.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRADGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            complement(59006..60883)
                     /gene="cas8a1"
                     /locus_tag="KQD82_000139"
     CDS             complement(59006..60883)
                     /gene="cas8a1"
                     /locus_tag="KQD82_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG4378645.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLESLVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQEYVSSICDVCKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            complement(60889..61626)
                     /gene="cas6"
                     /locus_tag="KQD82_000140"
     CDS             complement(60889..61626)
                     /gene="cas6"
                     /locus_tag="KQD82_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG4378646.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            complement(61704..62621)
                     /locus_tag="KQD82_000141"
     CDS             complement(61704..62621)
                     /locus_tag="KQD82_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG4378647.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            complement(62900..64816)
                     /locus_tag="KQD82_000142"
     CDS             complement(62900..64816)
                     /locus_tag="KQD82_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378648.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKEDVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            complement(64816..65562)
                     /locus_tag="KQD82_000143"
     CDS             complement(64816..65562)
                     /locus_tag="KQD82_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378649.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     YIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(65666..66517)
                     /locus_tag="KQD82_000144"
     CDS             complement(65666..66517)
                     /locus_tag="KQD82_000144"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG4378650.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQV
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(66765..67769)
                     /locus_tag="KQD82_000145"
     CDS             complement(66765..67769)
                     /locus_tag="KQD82_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4378651.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            complement(67779..68471)
                     /locus_tag="KQD82_000146"
     CDS             complement(67779..68471)
                     /locus_tag="KQD82_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4378652.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
BASE COUNT        22996 a        11586 c         7883 g        26040 t
ORIGIN      
        1 aatgtataat attatttgct atagaaaata atacactcat acataataaa ttttaggagt
       61 gaagtatatg aatcaagaat atgtaaaagg aaatcctatt tttactaata ttaataaagt
      121 tccaagacaa tttccatacc ttactgatga tattgatact gatgttataa ttgttggtgg
      181 tggcgtaact ggatgtattt gtgcttacta tctagctaaa aacaatataa agtctgtcat
      241 tcttgaaaaa ggaaggattg cacatggaag cactagcgtg acaacttctc tattacagta
      301 tgaattagat gacaatttga tagatttaac agaggttatg actctaaaag atgctctaaa
      361 agcatacaac ctttgtactt cagcacttga agaattagat acatttatag agctatatgg
      421 aaataagtgt gattatgcca aaagagatac tctactttat actgccaaca aacttgaagt
      481 taaggctatc aaagaagaat ataacttaag aaaagaaaat ggatttgatg ttgaatatat
      541 tgatgaatcc acaaaccctt ttagctttga tttaaaatca ggattgatag caaagaatgg
      601 aggacgagaa ttagacccat ataaatatag tcaccatttg attgatgtaa gtttaaaaaa
      661 tggtcttcaa gtatatgaaa atacagaagt aaaaaaagta gatttatcaa atgataaggt
      721 tactgctgaa gtatcgtatg gatacaaagt tcatggtaaa aaattaattg tagctacagg
      781 gtacaataca agtttattta caaaaagaaa ttttgcaact aagtcaaata cttttaatat
      841 agcaacgaaa ccacttaaaa acatagcttc ttggaaaaac aacattttaa ttagagataa
      901 ctgtgaccca tataattatc taagaactac taaagacaat cgattaatta taggtggtga
      961 agatgtaagt tttgatgata tagaaaatga aactttagcc aatgaaaagt atgatatttt
     1021 agaacaaaga ttgaaaagta tgtttaagga tataaaagac attgaagttg aatataaata
     1081 ttgtggttgt tttgcttcaa ctttagataa tttaggtttt attggtccag ataataaaaa
     1141 taacaatcta tggtattgct taggttatgg agcaaatggt atactctttg caatattagg
     1201 aggtataatg ttaagtgaat tatatcttgg caaacaaaat aaaaatatga aattatttaa
     1261 agtagataga tttgataaat agatattata tattaaaaga ctgtaaaaag atgaaacttt
     1321 ataattctaa aagttaactt tgatacagtc ttttataatg ttccttttaa aaatacttta
     1381 tttataatta aatataaagt aaaatgttaa aatataacta ataaaattta gtcataagat
     1441 aatatttaag tttatttgat gaaatatgga ggaattatta tgaaggaaat actcgttatt
     1501 gctcccacta aaggaaccta tgaaaaatca attcacatag ttaaaaaaaa caaatataca
     1561 aatattgatg ttgtatttgg caatctaaaa gaaggtatac ctcttgctga aaaaagtatt
     1621 aatcatggta ctagaattat cataagccga ggtggaactt acaatatgtt aaaagctact
     1681 tacaatatac ctatagttga aattaaagta gatgcttatg atattataaa aagttacaaa
     1741 gaggtaaaaa attccaacga accatttgga ataattggat ttaataatgt catctacggt
     1801 ttcgatatta tagaagaaat attgaataag aaaataacta tgattgaaat tgaaaaagaa
     1861 gaagaaattt atgatgctat agaaaagtac agaaagaaag gtataaacac atatatagga
     1921 gataccactg ttgcacatat tgttaagaga cttaattgta agggaatatt aatcgaatct
     1981 agagaagaaa acatacttag agccattcaa caagcagaac aaatactcga agcaaccaag
     2041 gatgaacaaa aaagaagatt acagatagaa gctatgacag attttgtaca tgatggaata
     2101 ataacagttg ataaaaattt taaaataaca ttatttaata agagtgctga aaaaatattt
     2161 ggtataaaaa aagaaaatgc actttatcat aatgttgtag atgttatccc aaacacagtt
     2221 cttccacaag tagttgagac tatggaacct caaattggtg aaatacaaaa tatagggagt
     2281 acaaaaatta ttacaaatag agttccaatc attgttgatg gtcagattca aggagctgtt
     2341 tcaacatttc aagatagcaa ggagataagt tcttttgaac atacaataag aaggagctta
     2401 ttaagcaaag gtctatctgc taaatacact tttgatgata taattcatgc tagtgaaaag
     2461 acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt atgatactcc tatgattatt
     2521 actggagaat ctggagttgg taaagaacta ttttgtcaaa gtatacataa ctatagtaag
     2581 cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca tacctccatc tctaatagag
     2641 agtgagtttt ttggttatga ggaaggttca tttacaggtg ctagaaaaaa aggtaaagca
     2701 ggtatattcg aactagcaca tgaaggtaca atcttcctag atgaaataag tgaaatccct
     2761 cttgaacttc aaggtcgatt attgagagta ttgcaggaaa aacaagttat gagaattggt
     2821 ggtgataaag ttattcctat agatgtaaaa attatctgtg cttcaaataa agacttaaaa
     2881 aatatgatgc gcaatggttt atttagacaa gatctttatt ttagaataaa tatactaaca
     2941 ttatatcttc cttcattaaa agaaagaaaa gaagatatta taaaattatc agaatttttt
     3001 atacataaat attcaagcaa atacaataaa acacccttaa ttatcactcc aaatattaaa
     3061 aaggcacttc ttgaacgaga atatgaaggt aatataaggg aattggaagg tatgatggaa
     3121 agggctgtta ttgtggaatc atttgactcg attttattag aaaaggttag caatgaatat
     3181 acaaataata tagttgataa agataatagt gattttgaat gcagtaattt agatttaaaa
     3241 actttagaaa aaaaatatat ctataaaatt tataacgaaa ataatagaaa tactacaaaa
     3301 acctgtgaaa ttctaaagat aactcgctcc actctatggc gtaagttaaa agaaatagaa
     3361 gatgatgttt aaaaataata catatctata tatatcaaaa cgttttatgt ttaattttaa
     3421 tacataagac gtttatttgt tttcaataaa aaatacaatt atatgataaa aagaatcttt
     3481 gtaagtgaaa ttctcgtcaa tcgtactttt aattataagt aatctacagc atttcaatcg
     3541 attattaaaa ataatcatga taactaaaga tacaaactct atatattggc atattaattg
     3601 cttttataat acctatataa aaatgaaagg gagtgatact agatgcacat cttaaaatca
     3661 gtgaaaaaga taccaggagg acttatggtc tgtcccttgt tattgggagc actatttaat
     3721 acagtatgtc cacaagctct tgaaattgga ggattcacaa catccttatt taaaactggt
     3781 gcaatgagta tacttgctct attttgtcta tgcaatggag ctcaaatcaa tatcagacaa
     3841 gctggtaaac cattgactaa aggtatagta cttacagcaa caaaatttat aattggtgca
     3901 gtattaggta ttattgttag taaatttatt ggacctaaag gtataattgg tattacacct
     3961 cttgccatag ttgctaccat gactaattca aatggaggtc tatttgctgc tcttgcaggt
     4021 gaatatggag actccacaga tgttggagca atatctatct tatctctaaa tgatggtcct
     4081 ttttttacaa tgcttgcttt tggagtaact ggacttgcaa atgtacctat agtagcacta
     4141 tttgcagcac ttataccaat actacttgga tttatacttg gtaaccttga tgaagatata
     4201 cgagaatttc ttgcacctgg tacaacatta ttaattcctt tcttcgcttt tcctttagga
     4261 gcagcattaa actttaatca aattataact gctggactac ctggagttgt acttggttta
     4321 ggagttacta taataacagg tatgggtggg tactttgtaa tgaaacttat acgtgcagaa
     4381 catccagcag taggtgcagc tacaggtaca acagccggaa atgcagctgg tactcctgaa
     4441 gctttagcag ctatagaccc tactctagct acaatagcag ctgtttcaac agtacaggtt
     4501 gctgctgcta ttattgttac agcaatatgt tgtccaatgt tagtttccta tctagataaa
     4561 agagaaaaaa agaaaaaatc tatttttaaa acaaataaaa atattgaagt aaattaatac
     4621 ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa tgaaaaagat aatctagtta
     4681 catgtgtcag acctttagtt aaaggtgaaa aagtttcaat tgatggaaag tcttacatag
     4741 taaatgataa tatacctgtc tttcataaga tggctacaga agatgtaaaa aaaggtgatg
     4801 ttgtatacaa atacggtgaa atcataggaa ttgcaacagt tgacataaaa actggtgact
     4861 atgttcatgt acataacatt gaaagtacac gtggtcgtgg agataaaaaa taggaggaag
     4921 ttaaaatgaa attaatgggt tatttaagag aaaatggtca gtttggtatt cgtaatcatg
     4981 ttttaattat tccaacatct gtatgttcaa gtgagacagc tacaagaata gcatcacttg
     5041 tgcctggagc aatagcaatc cctcatcaac atggatgttg tcaaattggt tctgatatag
     5101 agcttacagc aaagacatta ataggatttg gaaaaaatcc taatgttgcc gcagttttag
     5161 tagttggtct tggatgtgat ggtattcaag caaaagaatt agcatctgaa atagctacaa
     5221 caggaaaaaa ggtagattac gttgttatac aggaatgtgg cggaacatta aaaacagttt
     5281 ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt gtcaaaagaa gtaagagttg
     5341 aatttggtat gagtgaaatt acattagcac ttgaatgtgg tggttctgac ccaacaagtg
     5401 gaatagcttc aaacccttca ataggcgttg catcaaacct tcttgtagat gaaggtggaa
     5461 gtagcatact aagtgaaact acagaagtaa ttggtgcaga acatttgttg gctacccgtt
     5521 ttgaagatga agaaatgaaa gataagtttc ttaaatttgt aagtgatgta gaaaaaagag
     5581 caattgctat gggagaagat ttacgtagtg gtcaacctac tccaggcaat aaaactggtg
     5641 gactttcaac aattgaagaa aagtcacttg ggtgtatgta taaggcaggc aataagccat
     5701 tcaaaggtgc tcttgaatat gctgatattg tacctcctga taaaaaagga ttatatttta
     5761 tggatactcc aggtcaagat atagattcta tcactggtat ggtagcaggt ggtgcacaaa
     5821 ttgtaatctt ttcaactggt agaggtactc caacaggaag ccctatttca ccagttatta
     5881 aaatcactgg gaatagtgat acatacaaca aaatgccaga caatatcgat ataaatgcag
     5941 gtagaattat aacagatggt gctaaaatag cagatatagg tcaagaaata tttaatgaaa
     6001 taatagaagt ctgtaatggt aaacatacca aagctgaaag tcttggtcat agagaatttg
     6061 gcatttacag aatttcatct acattttaga ttgtaacaaa atatctttta taaatataat
     6121 acttatgtag atacataagc taaaagttct ttctaatact ataaaaattt tctatactga
     6181 aaaggaactg tatcaaaatt attttgaaaa cagttccttt ttttaacctc ttttactcaa
     6241 aatattataa aataagattt aaacaataaa cgtatcttga ttaatctcta agaagacata
     6301 aataaagcta aatgtaaagt gggataaata aaatttaata tctttgtatg aaaagatatt
     6361 tggtataata tgtctgagag aattcttatt tgtaaacaaa taaaggatgg aggatgaaac
     6421 aaaagaatga actttgaaaa cacaaaaaat aaaaaaataa tagctacttt actattgaca
     6481 ctccttttaa tcgtgtcatt aacatgtgtc acttttatta tgaatctaaa taaagtaaca
     6541 cataatgaaa cagaacgata tcttgtagaa gtttcacaag gtatttccac tacaattaat
     6601 gtgaaatgta aaagtatatt taggacacta tattctatgg gagatactta ttttgagatt
     6661 aatgatagtg aaaaagaagt caatagatac ttatcaaaaa aattaaaatt atttgaattt
     6721 gattggttag gtattataga ttctaatgga aattttgtaa gtgcccttaa agatacaggt
     6781 aatgttaaag gacaagactt ttatagaaaa gcccttcaag gcaaagaaac aattactcct
     6841 gttaaatcac aatctggtac atatggaatt ttatattctg taccactata caatggcgat
     6901 aaaattgttg gagctgttgc tgcttggaat actattgata ctatgagaaa tttattaagt
     6961 gtagaaagct ttcatggtga aggattttcc caaattatcg accaagatgg aaattttatt
     7021 gtaagttcag caaacaaaaa tgctccaaaa aatgtggtaa atttttttga catgattaaa
     7081 caccaaggaa aacaggttga taaaaataaa cttgaagaaa tgaaaataaa cttaaaaaag
     7141 aacaaaactg gttctatgta ttatacatta aatgatggta tttctaaggc aatgcactat
     7201 cttccattac aagaagaaaa cttatacctc ctttcaattg ttccaacaaa aacagcaagt
     7261 gcacagtttg atttacttct aaaaaaagca atcttcatca atattttaat tgttatacta
     7321 tttttattgc ttattatatt gattatgttt ataaatcaaa agaacagggc acaacttata
     7381 aaaatcgcct atgtagactc tgtaactaaa ggttacagta aagcacgttt tgatattgaa
     7441 gctaaaaaaa ttatacagtc atcttcatca gaaagctatt cattggtatc tataaatatt
     7501 cagaaattca aacttattaa tgattctttt ggtagtgaag caggagataa aacattaaag
     7561 tacatacatg atgtcattag taattatcta gaaccaggag aattgctttg tcgagtttct
     7621 aatgaccagt ttaatgtact tattaaaact attcctcaaa aagaaattct taagtacata
     7681 gaaaatatca ccaatgatat taatagtttc aataaaaatg aagaatgtaa atattttata
     7741 gctctatcta taggtgtata taaaattgat gacccaaatc tacctttagt ttcaattcaa
     7801 gaccgtgcaa atgttgcacg aaaaaataat aaaggaaaca ttggaaatca tccactttat
     7861 acatgtgttt tttatagtga tttagagcgt ttaaaaatga ttaaagaaaa agaaatggaa
     7921 aatagaatgg aggaagcatt aaataataac gaatttatag tatatcttca gcctaaaatc
     7981 agcctaaaag ataatactat tgtaggtgca gaagcattag taagatggca agacccaaat
     8041 aaaggcctta taccaccaga cgagtttatt cctttctttg aaaaaaatgg ttttattaca
     8101 aaactcgacc actatgtttt tgaaaaagta tgtagtatga taagaaaatg gatagataat
     8161 ggagaaactc caatacctgt ttcagttaat ttttcacgtg ctaatttaaa taatatagat
     8221 tttttagaaa aatacagaat gcttcgtaat aaatacaaca ttcctgcaaa gctcttagaa
     8281 attgaattaa ctgaaacact tgtatttgaa aatctccaaa aattaattaa tataatagac
     8341 caaatccatg aagaaggttt tcaatgttca ctagatgact ttggaagtgg atactcttca
     8401 ctcaatatgt taaaagaaat aaaagttgac acattaaaac ttgaccgtgc tttttttagc
     8461 tcacctaatg cagataatat tgctgaaaat catgtaatag aatctatagt taatttggca
     8521 aaaaaattaa atatgaatag tatatcagaa ggtgttgaaa caatcttgca aatggattat
     8581 cttaaaaaaa ttaattgtga tatgatacaa ggatatgttt tttcaaaacc tctacctcaa
     8641 gaaatatttg agaaaatgac ttttggaaaa aaactagaag aaaccagtat ggatagttcc
     8701 gtttaaataa aataaaaaac ttgtctctat aaaataattt taatcatttt ataaagacaa
     8761 gtctttttat tttccaattt aattttattt tctaaatttc ttacactcgg cttgttttaa
     8821 attccctttt taagaataat aatacgatta tcatagcaat aaaatctgca actggctgtg
     8881 ccaaccaaac accatctaaa ttaaattgaa ttggaagtac aatgattaat ggtataaata
     8941 aaataaattg tcttaaaaga gttaaaaaca ttgaatgttt aactttactt atacactgaa
     9001 aatacacagg tcctacaata gaaatcccca ttataggcaa tgtaattaga tttaggctta
     9061 tacctcttac tgctatatcc atcaaatctg aatctttatt aaatatacct acaaatactt
     9121 ctgggaaaac ttgtactact aaaaatccaa atgacaatat gacaactgac actatgatag
     9181 ataatattaa agttttcttt gcacgctcat attgttttgc accataattg tatgatatta
     9241 tagtttgcat accttgattc aatccaaaca caggcatcat aaacatcaat aaaatagatg
     9301 taagtgctgt catagctcca atagctaaat ctccaccata aacctttaga gaattatttg
     9361 taaacatgtg agttatactt atagctactt ccatagcaaa aggtgtcatt cctattgcaa
     9421 ataccttctt tactatatct ttattcaatt ttaagttaca tttttttagt tttaaattgg
     9481 acttccctct tataaaataa tgtattaccc aaaagaatac tactatttga cagagtactg
     9541 ttgctatagc tgctcctttt atacctaaat taaatacaaa tataaatata gggtccaata
     9601 tcaggtttaa tacacatcct acaatcattg tccttgctgc taattttgga ttaccttctg
     9661 ccctaattgc attattaaga taaaatgcaa ctaaattaaa tatacttcca accaatatga
     9721 cactcatata gtcttttgca taaaatattg aatctttact tgcacccaaa ataaaaagta
     9781 tatcttctaa gaaaactaat cctaatatag ttataattaa agagataatt atagataatg
     9841 tcaaagcatt acctaatatc ttctctgctt cttctctatt tctttcacct aactttatag
     9901 acaatcttgt gctagcacct actgatacca acattccaaa agctattatt agagtaaata
     9961 ctggcatagt tacaccaaga cctgcaatag ctatagagcc aataccttcc atagaaccaa
    10021 taaaagctct atctactaca ttataaagtg aagttaccat cattgctatt attgcaggta
    10081 tcgaatacct aaataataaa atccatattt tttcatgtct taatgcttct tgattctcca
    10141 tgttgtcacc atttacacca tgtcataagt tatacaaatt ggtttatttg ttcttgggtc
    10201 tgttgctatt tctgcatcaa tattaaataa ttcttttaga ttatccttcg tcataacatc
    10261 atatgcatca ccttcacaaa ctattttacc tttcttaata cctatcatgt ggtcagcaaa
    10321 tcttgctgca ttattaagtt catgtattac catgacaata gttcttcctt gttttttatt
    10381 tagttcatct aataatttta gtatctccaa ttgatgtgcc aaatctaaat atgttgtagg
    10441 ctcatccagt aataatatat cagtttcttg tgccaaagcc atagctatcc atgccctttg
    10501 tctttgtcct cctgataaat cttccatatt tctatcctta aattctgcaa ttccagtaac
    10561 ttctaaactc caatttacaa tctctctgtc ttctttacta gaattaccaa atccactttt
    10621 atgaggaaat cttccataag aaattaattc tgaaacggta agaccactag gagcttgagg
    10681 gctttgtgga agtacagcca tatcctttgc tatttcttta gacttctttt tgtagatgtc
    10741 ctctccattt atatatattt tcccactatt agggctaata attctaccta tagttttaag
    10801 tattgtcgat tttccacaac cattagcccc tatgatggta gttatttttc cttttggtat
    10861 atttaaattt aaatttttta taatatctgt gtttccatat gatacattta gattcttagt
    10921 atttatacag ttcataacaa caccctttcc taatcattta ccaacattaa ataaataaag
    10981 tacggcactc ctattataga aactactata cctactggta tttctatagg tgctagaaga
    11041 tttcttgata tagtatctgc tattaaaagt aataatgttc ctatcaatgc tgatactggt
    11101 attaatcttt tatgctttgg tcctattatt tttctaccta tatgtggagc tattaaacct
    11161 aaaaatgaaa tactacctgc tactgctgtc gatacaccag ataaaattac tgcatatatt
    11221 ataagtcttc ttctttgttt ttctacatct acaccaagac ttatagatac ttcatcaccc
    11281 aaatttaaaa catctatata tttagattta taaatagtta tacccataaa aatcactata
    11341 aatggaagca cagctattac atatttccaa ctggttcccc aaatgcttcc tgatgtccat
    11401 gccataaccc tattaaattc ttgtgttgta aatttcaatt ggaatatcgt caaaattgaa
    11461 gtaaaagcta tgtttacacc tataccaata agtagtaatc ttgacgaatt tataccacct
    11521 ttccatgcaa gagaatatat aagaaatgct ccaaataaag ctcctataag tgcaacaata
    11581 ggcattgtaa atacagtcaa gttactcata ccttcatata cattaccatt cataagatat
    11641 atataaacta ctacaaacaa tgcagcacct gaatttattc ccaaaattcc agaatcagct
    11701 aaatcattct ttgtaactcc ttgaagtatt acacctgctg ttgataatgc agaaccaacc
    11761 aaaagtgcta ttactattct tggtaatctt aatttaaata ttgcaatttc atgattacgc
    11821 tgacccttcc ctaaaaaagt ctttataaca tccattggtt ctatagaaaa tgagcccata
    11881 tttaaactca ataaaaaagt gataaaaata agcaccatta aaattgatat aacaagtaat
    11941 gttttagaat ttatttttat aatatttttc atagtaccta tttctccccc tttctaacaa
    12001 ggtatataaa tattggcact cctataagtg ctgttataga acctactggt gtctcatatg
    12061 gaggatttat catcctactt aatatatctg tatatacaag caatacagct cctaatacta
    12121 aagagctcgg tattatatac ttataatctg aacctactat tcctttagct atttgaggaa
    12181 ctataagacc tataaaaata atattaccag acactgacac agatgctcca gtcattaata
    12241 ttacaagtac tatacatata aatctaacaa agttagtttt ttgtcctaat cctattgcca
    12301 cttcttctcc aagacttaat atagttattc ttggagccat aataatagct aaaataaatc
    12361 caataccacc agctacaaat agcaatttta taccttccca cttcactcct acaagccctc
    12421 ctgatatcca gaaacttaat tgttgagata ggttaaaata catagatatt gctgatgcca
    12481 aagaaattag caatgtacca agagcgactc ctgcaagagc taattttaca ttattaactc
    12541 ttcctcttga tttagaactt ataaaatata taaataatcc acttatactt gctccaataa
    12601 atgcaaacat cattacacta aaacttccat gtataagttg aggaagtttt ttttgaagta
    12661 ccagtgatat cgaaattgcg aatgtagctc cttgtgtaat tcctataaca gatggttcag
    12721 ctataggatt tctcgttatt ccttgcatta ttgccccaga aactgctaaa aaccctccaa
    12781 caagtgcagc tgctattgcc cttggaagtc taacatcctt tactattctc atatttattc
    12841 catcttccat ttttatcaaa ctatctataa cagttgatag attcatatct tttgccccaa
    12901 tagttatgga taatacaatt ccaaatacaa gtacaactat acttactaat atcaacaaat
    12961 aagttatatt ttttttactc agcttagtca attatcctac ctccttttcc cttttaatcg
    13021 tcagtataat atcatctatt aagtatttta attttaagat ttattaaaaa taggtgtgaa
    13081 aaatgaatat cattatctca cacctactaa gttaagaatt actttgttaa ttcattttta
    13141 actgattcta ataataactc ttttccaatt ggattgtatg attgactgaa aaatggaaca
    13201 gcatcaagta ttgttacatt accatctttt actgctctta tttgagccca gatagcacta
    13261 ttttctaaat cttttttatc tgcttctgta gcaattacaa ctatatggtc tgcatctata
    13321 tctgttaaac cttccatact taccattggt agtgttattc catcctgttt tggcatattc
    13381 ttaggtctag ctaatttcat atcatcattg attagagtac ctattccacc atcactaaat
    13441 accataaatt gacctgaact tgccaacact ggtaaataag ttttttctcc attcttttct
    13501 ataacttcct tacctaactt tgtagctttt tcatcatact tctgtagcca acttttagct
    13561 tcttcttctt tgtcaaataa ctttgaaaca tcagttaact tgcttctcca gtcatttgca
    13621 taatctttca tcataactac aggagctatt tcttttaatt ggtcatatat tttttcttgt
    13681 ctttgactca ttattattaa atcaggattt acttctaata tagcttccat atccatagta
    13741 tccatcattg agtgcccaac tatctttaca cctttaagct cttctcttat gtaacttggt
    13801 aatttatcag tttcatatga gtctacattt gctgttgcaa caggcttata acctgctaat
    13861 aatagttctt cactacttcc tgatatatca actatttttt taggatttga tggtatctta
    13921 acctctcctt taacagattg tacaactctt gtctctgatt tatctttatc ctctgtattc
    13981 ttatctgaac atgctgtaac aagtactaat gtagttataa tagatataaa tattgcaagt
    14041 gattttattt ttttcatgat tacttctcct acttgtttat tatacttaca tgatattgat
    14101 tatcataagc acatatttat tatagcattt ttctattttt agtcaatatt attttgtaaa
    14161 aggataaaaa ttcctaatta atcttaatta tatatagctt agataaggtt ttcttatatt
    14221 aaacagtatt tttttaaaga attggacaca aatacaatga cttttcagtt aaacaatgat
    14281 aaaatgcaat ttatagataa tgaatttagt aaggaggatt caaaacatgg tacatttagt
    14341 atattgtgat aatgcaggaa aaaaaggaga aaaggtctta gataaaatct tggctggaac
    14401 aaaaacaatg gttattagag gtgctgctgg tagaaaaata ccacatagta gagtatttga
    14461 agatgagtta ctttatttta tggaaaaagg aactgcaaaa atatctgcaa aagccactgt
    14521 taaaaaggta gaaaactatg ttaaattaac agaagatgaa atttctaaaa ttttaaatga
    14581 aaatcaacca aaattaaatt tatctgaaaa gcaaaaagtt cgttggcata aaaaatgttt
    14641 atgcttagtt gaatttgaaa atgttgaaga aataacacct cttgattttg accatcaagg
    14701 aaatatggat gattggctta ttgttgaaaa aattgaagat gtagttgttg gtactagtat
    14761 accttacaat tatgagaagt ctaaattcta aagtatcaat aacataatac tatattgcta
    14821 aactctactt aaataaaaaa agagtgcatt taataattat aaaaattatt tttatacact
    14881 cttttttaat aataaattta aattacttac tttctaatct ttcatataca tctataaatt
    14941 cttctgctaa ttgttgaaaa ttcatagctg tcattaaatg gtcttgtgca tgaataagta
    15001 taacacttat ttcagtttta ttaccattag cttcattttg tatcaattct gtttggaatt
    15061 tatgagcttt atgaattgat tctttcgact tttcaataca ttctctagct ttatctatat
    15121 tcccgctctt tgcttctttt attgcttcaa atgctagccc tttcgcatca cctgcaaatc
    15181 caattattcc aaaagatatc tctatcatct tctcatccat ttattcgacc tcctaaatat
    15241 ctctatagct tgatatgact aatccattct tttctaaaaa ttctttgact tttttagatg
    15301 taagtatctt gtgctctttc gttctatcaa tagcataaga tgtagaattt aaaatataat
    15361 catctaaaaa agctgggtga gacataatat ccactacatc atacttcatt atttcgtcta
    15421 tattttttat aaaatattct tcacttacat tttccttata aaaactatct attaaaggaa
    15481 ctactttaga atactctaaa ttatattcaa atccacctct tatcggaaga ttatacttgg
    15541 ttacaatctt ctctattact ggctttaaac ttactaaagt atggatatga tggtgactat
    15601 ctaaatgaga tatatctatt ccaagccctt ttaccctatc tatttgagca caaagttctc
    15661 tataaatctc atcacaatcc attttttcaa taatttcgtc tgtaatcctc ctaataaaaa
    15721 aaccgtcttt atcaacaata gtttttaagt tagataataa tggtctacca caacttaaag
    15781 tcatatgaac tccacaattt aaagttttat tttctttaag taagcctact ccatgttcta
    15841 ccccaggcat atttgccatc atagaagtag acctaactat accattatta tgagctgaaa
    15901 ttattccata gttcacagct tcacaatatc caaaatcatc tgcatttatt ataattttag
    15961 tcataccata acctactttc tatagacaac tatctattaa atctaggaag ataatcttta
    16021 tgagcttcca acatttcgtc taatatagtt tttgctaagt catcagatgg aatcaatggg
    16081 tttgtacaaa gtgccaaaag agcagattca taatcgccat atacagctgc tttagctgcc
    16141 aatacttcaa atgatttgat ttgacttact aatccatgca cagaatctgg taaatatcct
    16201 atacttattg gttttggacc attttttgtt attacactac ttacttctac tgcttgttca
    16261 tctttaaaat ctcttataga acccttgttt aatgtattaa ctacttggat atcttttttg
    16321 tcattgtata tagaacttat taaattacaa gcagcatcac tatagtaagc ccctcctctt
    16381 ttttcaagtt gaggtggttt tacatctaat ttttcatcct tatataattc aaataattgt
    16441 tcttccaatt ctttaactac ttgacctcta gcttttccct ctttaaactc tctcaactca
    16501 tcctcaagca tttctttagt tttatagtag tatctatgat atggacaagg tattgcaccc
    16561 aaagatttta caaattcagc attaaaatca attgccttta tattttgcat acttatatct
    16621 gctttgacaa atttatcaat tgcttctttt gtaacatctt ctccatctaa agatacattt
    16681 agaccataaa ccatatgatt aagacctgca aaatccatac ttattctgtc actttctaca
    16741 ttaaatagtt tggcaacatc attttttaaa tgaattggca cgttgcatag acctatatat
    16801 cttctaaaat tagtatattt gaatacagcc tctgttatta ttcctgttgg atttgtaaaa
    16861 ttcaccatcc atgcatttgg acataattct tccacatctt ttattatatc aaatataact
    16921 ggtattgtgc gaagggcttt aaataaacct ccagcaccat tagtttcctg ccccataact
    16981 ccatgagata aaggaatagt ttcatctttt attctcgcat ctaaaagacc tactctaagt
    17041 tgtgttgtca caaagtctgc atctcttaga gcttctcttc tatctaaagt aagatttatt
    17101 ttcatatcta ctccagcttt ttttaccatc ctcttagcta gatttccaac tatctctaat
    17161 ttttcttttc cagcttctat atctacaagc cataattcct ttacaggtaa ctcttcatat
    17221 ctatttataa aaccctctac aagctcagga gtataacttg aacctcctcc aattgtaact
    17281 atttttagtc cctttttcat ctcattatac catccttacc tataatttaa tatactctac
    17341 aaaaccctat acatttattt accgaaataa aactacctag ttgttaattt tcaatttatt
    17401 agcttccatt ttttcagacg ctatgacaaa tggaatatat attaatattg ataatattaa
    17461 attaaatagt gctaaagctc caccagtcca gtcacctgta gccattgctc cacctaatat
    17521 aggaggtgtt acccaaggta ttttaggtaa aattactggt gctactaatc caaaatctat
    17581 tgctaagtat gatacagcac tacatattac tggtgcaagt acaaatggaa tcataaatat
    17641 aggatttaaa actattggaa gtccaaatat caatggttct cctatgttaa atattgcagg
    17701 tggaccacct aatactatca tctctttacg tcttcttcca gctattaata aagctactat
    17761 taatcccaat accattccag aaccacctaa atacacaaat gcatcaaaga atgcacctgc
    17821 aagtacatgg tatccttccg ttgctcctgc ttgagccaag gcagcatttt ctccacctaa
    17881 tttcataagt atagtttctg taaaaggaag tgctatattt gcaccatgaa gtccaacagt
    17941 ccaaagtata tgaattataa atgcaataag cattgtagaa cctagactat cagctacatt
    18001 tgatagtggt gctcctaaat aagtatttaa aatatctgtt aagaaacttc cattagaaag
    18061 catttttata aatattccta ttacagtaaa tatcattata gttaacattc ctggtattaa
    18121 ctttgcaaat gatcttgaaa ctgctggtgg aactccatct ggcattttta tagttaattt
    18181 ctttacctta gataatctta caaatatctc agttgataat aatcctatta atatagaaga
    18241 gaataaagct gctgtaccta agtaggtttg ttgtatcatt ccccatcctt caactactgt
    18301 agttcctccc tcaggaacta ttttaccaat ttgtggagcc attataaaaa agatagataa
    18361 agctataagc ccagattgta acccatcact ctcataagat tttgctagaa aataagcaac
    18421 acctattact aagaatactg ccattgtacc tattgctccc caccaaaggt cgcctccaaa
    18481 tgtggtccag ttttctccaa atatacttgc catcaaattt ttgtatgctt ccaatggtaa
    18541 gttatttatc aatgttccca atgcacctac catagttata ggtatcatta ctataaatgc
    18601 atctcttact gctactaagt gcctttgagc tcctatttta gcagctacag gcactatata
    18661 tttatccata aatgacatga atttttccat tttacatcct ccagtacaac tttattttga
    18721 actatttaac tctacagctc tatctaacac agcttttcca ttacatcttc cataatccat
    18781 catatttata acttctactg gaatattata tggttttgcc atttcagttg cttttcctaa
    18841 tacatatcta acttgaggcc ctaaaagtaa gacatcacaa ttttctattt cattttttaa
    18901 gttcacttct gatatagccc atatttttgc ttctataccg ttttcctttg ctgcatcctc
    18961 catttttgta actaataaac tagttgacat tcctgctgaa caagctaaca ttatttttat
    19021 cattttttcc cctccaaatt agattcatta aatatttttt aagtcataag ttttttaaac
    19081 tatacattta aaatatgctt ttctattaat tttttcttaa tgtatatatc ttatttttaa
    19141 atgtatatac atttaattct tgatattatt atataccaaa tgtatataca tttcaagaaa
    19201 actttttcat tttatttaaa ttttattcga caacagcatt caaagttaat gtatatccat
    19261 agtgatatgt gtgtgagtac tcaaaaatct cactattttg taacttacaa atttggctta
    19321 tatataaaag tggctctggt tcttctaatt ttagattatc catacactct tcattcgata
    19381 ttacagcttg tacttctctc tctgacatgg ctatcttgta tccacacact tcttcaacaa
    19441 aattataaag agaattttca caatgagtct tatttaaatt tggaaataac ttaactggca
    19501 tataagtaat catatatgaa actcttgcat cattggctaa tcttactctt tttatttccc
    19561 aaacattttc ttctaaactt atattcaact tacttgataa attttcatct gcctgaacta
    19621 tttcaagaga taccaatata ttagatactt tatagccttt actttccatc tctttagtaa
    19681 atccacttac agacgatata ttatcatata tattatttgc aataactata ctacctacac
    19741 ctctttgtct agttatatag ccttctttaa ctagattatt aagtgcagac cttacagtca
    19801 ttctagttac attaaattct tcactcagct gtttttcaga agggattaaa tccccttctt
    19861 tcaaactgtc tgaatttatt aactctctaa catgattttc tataacttta taaattggct
    19921 ctttcattta attcactcct tattaaatct tttatataat aagataattt tatatacaaa
    19981 caaagaaaat gtctatacat ttaagataat ttaaatttta ttcagaaatc ttgtacaact
    20041 ttccgagttt taataaagat ataaatagac ttatcattaa tatacctttt aatatacttg
    20101 ataaggttat actccaccat acaccattta gacccaacat ttctggtcta gatataaagt
    20161 aagccaatgg tactcttgca ccagtcagta tagtaattat aatagaaggt atataggttc
    20221 tccctaatcc tttgaaagct cctgttgtta ttatttctaa acacataaaa aattgagaat
    20281 agcctaatat tttaagataa actgctcctt tttctattgc ctcactttca tctataaaaa
    20341 tagaaaatat actctctcct gcaaacacta atactaaagt agttattata ccaagtacag
    20401 aagatacagt tatagctatt ttgcaacctt tatttattct agaatacttc ttggcaccat
    20461 aattttgtcc aacaaaactg cctaatgcaa ctgatagccc ttcagccgtc atccaggaaa
    20521 ttgcttctat ttgtgagcct actttttgta ctgcaacagc cacaggcccc catgatgcta
    20581 ctattactcc taatagcatt gagaatactg taaacatacc actttgtatt gctataggta
    20641 atcccaattt atacagaact ttataatatt tagtctctat ttctttaaat agtcttaatt
    20701 taaaatattc ttctttactt ttaactatta tatatataaa acatgtagta actactatct
    20761 gagcaaatac agttgcaata gcagctcctg caacaccaag ttttggagca aatccccatc
    20821 caaaaattaa tatagggtca agtataatat tagttattaa tcctactgta ttaatacgaa
    20881 aaggagtttt actatttcca agaccattga aaattgcagt aaatactgga ttaataaaat
    20941 aaaataccat acctagagct actattatca aatactgtct agacatggtt ataacttcta
    21001 agtctcctaa tctaaaaaaa cctatcaatt gtttattaag tactattaaa gaaattgtat
    21061 atattattgc tagtataata tttatttcta ttgctgactt catatatgat ttagtagtgc
    21121 ttatattatt tgcaccaata ctttgtgcta cttttatctc tccacctact tttgaaatca
    21181 taataaatgc cattgcaagc catggaaaaa aaccagctgt tccaactgca gctactgctt
    21241 tacttccggc tttaccaacc catatcatat ctatcatgtt ataccccatc tgtataaatg
    21301 aagtccccat tattggaagt gatagcttaa gtaatttttc tgtaattctt ccttctgtta
    21361 aattaagtct gtctttcatt ttccacctct tttatattaa atttatttgc ttatatagct
    21421 agattaatca ttttatttga ttgagttcac tatccttcaa tttatattat catatctcag
    21481 gattgcatct caatcatact tatgtttaag atacagtatt ttacctatct atgataaaat
    21541 taatacttat gaatataaat ctagccattt catcaataca tattctaacc cttaacttaa
    21601 cttcaattaa gtatacatat cgtaaccaat tagaatattt tatacggtat cttccattaa
    21661 taaacaacca atatcaatga tttatatata attttattta tacataaaat agtatactat
    21721 aataataaaa aaactatatt tgaatgcttt aagcatgttt actaattgta atttccacac
    21781 aaatttgata atatatgaat atattatggt gaaatttata gatatatatt aattatgggg
    21841 agattgtggg aggttctttt cacaagacat ttaagctatg aaaataaata aaaaattcaa
    21901 tagagttgat atgcaggttt caatacttac tgcaattatt gtaataatat cttcctcatg
    21961 tgtatatttt tttaattata cactaactta taagagcatg atatactctt taagtgagag
    22021 gtcaaaacat atctatgaat atgtagaaag aaatattgat aaaaatacct ttactgaaat
    22081 taattctaaa gaagaccaat caaaaaagtc ttataaaagt tctaaaatcc ttttagaatc
    22141 tgtaaaaaat actactggag tcatgtatct atatactgct aaaaaaacaa aagatggatc
    22201 actagtatat gttgttgatg gtttaaattc atctaggagt gattttagaa atgctggtga
    22261 tttgatagaa aaagagattt ggggagacct taacaaagca ttagataatc atatattgtt
    22321 acctaaaaag attaaatcaa catcttgggg atacatattt atatcgtatt ttcctattca
    22381 caatgatgtt ggtaaggttg ttggagtaat aggaattgaa tttgaagcaa aacatcaata
    22441 caatacattt aaatttataa gtatagttac accaataata gcattattaa cttgtctaat
    22501 atcagcattg attgcagtga ttctatttaa aagaatatct aatcctacat atactgattt
    22561 tgctaataca gattatttaa caggcctaaa aaatagaaat gcttttgaaa tagatatgaa
    22621 taacttaaac aattcaaatc taaagaatct aatatctatc atatcaatag atttagacgg
    22681 tttaaaaaag attaatgata aatttggcca tcagacagga gacttatata ttaagcaagc
    22741 aagtaaaatt atacaatatg taataaataa aaaatatata gtttatcgta ttggaggtga
    22801 tgaatacata attattctaa aaaatttatc tcacaaagaa ataaataata ttataaataa
    22861 tattaattta aaaatagttg aagaaaactc aactaatgaa ttaaaattat caatgtctat
    22921 tggatatgct atttttgatg aaaaagtaga tgtttcttta tttgatacat atcgtcgtgc
    22981 agattctcaa atgtatgata acaaaaggaa aattaataaa caatatcaat aataaattta
    23041 aaaagtctat tctttaaata ataaatcaca agaatagact tttttaaatt tgtgttttta
    23101 taagataaat tatttaatta aagtttgttc ttttttctaa acgattcttt ataaattcat
    23161 atattacttt cactaatata tttgtgacta atattaacac agctagtgct gctgcctgtg
    23221 cagtactccc cttatcatct agttgtacaa ttgatattgc agctactttt gaacttgcag
    23281 tatatataaa aactaatgct gatattgtca ccatggagtt taggaaaaag tacataaagt
    23341 tctctaaaac tgctggcaaa caaagtggta tagttacgtt cttaagaatt gatgcgctgt
    23401 ttattcccat tgatttagct accatatcta attctttatc tactttctta atagcagtag
    23461 tagctgttat aaatgctact gaaaagaaat gaactatatt acatataacc attataagta
    23521 atgttccata taatccatta aacaaattct taatgtatat tgtatccata aaattaaact
    23581 ctaatttatt aaaaaataat acataagcta tacctaaaac taatcctggc aatgccatag
    23641 gcaatgtact taaaaaatac ccaaatgatt ttaaatatgg agctttttct gttttttcat
    23701 tcaaatatgc aaataagaaa acaaagctcg tccctagaat agctgtaaat agtgacattt
    23761 ttattgaatt tataaatggt ttaatcccat caatactgtt tttcatagaa aaatgttcca
    23821 gtgtcagtct taaatcatat ggccaaactg atattaatga agataagatt acagttaaca
    23881 ttacagctat tataccaaat gcaataattc ctacaacccc tcctaaaata ctatctctta
    23941 acttattagt tttaacctta tatggaattg actttgaagt tatatatgaa ttttgatttt
    24001 tttcaatttt tttagttact ataaacgata ttattgcagg tattaaaagt aatattgata
    24061 cagttgctcc cattggcata ttctgttgcc ctataatttg cttatataca tctattgata
    24121 atacattaaa ctctcctcct atcacctttg gagctccaaa atctgtaaaa cataatgtaa
    24181 acgatacgaa tgcagaactt attaaagagt attttatact tgggattgtt atgcttataa
    24241 attctcttat cttgcttatc cccatagtat tactagcttc atatagtcta taatcggttg
    24301 aacttagaga tatataaagt atttgaaagg attgaggaaa tacatatata atttgtgcca
    24361 atattattcc cactgctcca tatattggaa catgcactcc aagtaaatta gttaccaaac
    24421 cttgatttcc aaataaatat actagaccta ttgcatgcat cattgttgga gcaaacatag
    24481 gaaacatagc taatgattta aaaaaacctt taaatttaat ctttgttcta gttaatgcat
    24541 atgcatatac taatgcaagt gttagtgata ttacagttga tataactgat acaaatactg
    24601 tattttttag agaatttaat agagattcag aagtcaagta ttcttgaaag ttttgtaatc
    24661 ctacaaagac tccatcttta tctaaaaaag cctgtacaaa tagatatatt ataggtgcta
    24721 ataaaaatat tactaaagac ataactaata ctgtaagcat acattttttt atatctaaat
    24781 tcatttttga tttaagactt atacttttag aacttatagc attattattc atacacttat
    24841 gctcctttaa cttttggata ctttaaatag tatttttcgt ctatacttat ttttacaagg
    24901 tcaccttcta ctaaatttaa attaacccac tcttttatag aaacatcact tattatgaaa
    24961 ttttcattta atttatcttt tatttcaact ctcaataaat tccctctaaa ctctatattt
    25021 gatattatcc cttggtagtt ttcttttgtc gatttctcta tttgaacata ttctggtctt
    25081 acagttaata tagaatctcc attatcaaaa cagtttgtat ctcctataaa ttgagctgta
    25141 aataggtttt gaggattttg gtatatttct tctggagtcc ctatttgcat tatatctcct
    25201 ccatttagaa tagctatttt atctgccata gttatagctt cttcttggtc atgtgttacc
    25261 attattgtcg ttatattaag ttctttttgt aatcttttaa tgtccattct aagcttgtgt
    25321 ctaacctttg catctaatgc agacattggt tcatctaaaa gtaaaaactt tggttcaaga
    25381 gctaatgctc ttgctattgc aatcctctgt tgttgtccac ctgataatgc tttagggtat
    25441 ttatgagctt cattagtcaa tccaactgtt tcaagtactt ccttaacttt attatctatt
    25501 ttttcttttg aaacttttct ctcttttagt ggaaaagcta tattgttata agcagtcata
    25561 ttcggaaata atgcatatga ttgaaatact ataccaaaac ctctctttga tggctctaaa
    25621 tttgtaatat ctttatcctg gagaattata gttccactat ttacatcttc aagacctgct
    25681 attattctaa gtaatgtagt tttaccacat ccactaggtc cgagtaaaca tagaaattcc
    25741 ccttcttcaa tatccaaact aatattattt aaaacctttt tttggtcata acttttaaac
    25801 acattattta tttttaagta actcatatct tatctctcct tatatagtta tttttttatt
    25861 ttaaatttta tatcctactt tttcttctaa aattctttca tctaaattat taatttaaac
    25921 tttacttact ttcagatttt gaaccgtatt ttgattccca agttttaagt actgtacttc
    25981 tgtttttggc agcagagttt aaatcaactt cttttataag ctgttcaaat ggttgtttag
    26041 aataaccttt tggaactgga ttatctgtct ttatggaagt aactgctact tcatgtgcat
    26101 atctatccat agctgaatca cttattgccc aatctaaaaa tagctttgct tcttttttta
    26161 tattatcttt tttaactaag gcatttgctt ctaagtccca acctgaacct tcctttggaa
    26221 atacaacctc tataggttct ccactagata attgttgtgt agctctatat ccaagtgtaa
    26281 tacctattgg atactctcca gagccagcta acttcgcagg ttttgaacct gagtgtgtat
    26341 atgtggctat atttttatgt aatttatcca tgtaattgta agcatctttt tcacccatca
    26401 tttgaataag ccctgatact gtaagatacc ccgttccaga tgatgctgga tttggcattg
    26461 ttataagtcc tttatattct ggttttatta aatcttcata actttttgga atatctatac
    26521 ctagtttctt aagttcctta taattaacta ctattgctgt ctctgggaca ctcattccta
    26581 cccaagacac atcatcttca gtatctttaa attttttatc aactttatca taatttttag
    26641 gcttatatgc ttcaagtgca ccatcttttt ttagctctat catgttcatt gctgataatc
    26701 cccatataat atctgcttgt ggattatctt tttctgctaa tagttttgaa gctattacac
    26761 catgagaatc cataactatc tctacatcta tattgggata ctcttctcta aaaccttcca
    26821 tataataagc tatctgctct tcctctagtg ctgcatatat tgtaagttta ttatttcctt
    26881 ttccttgagt agtttgtggt gttgttgata tgcaacttgt cataaatgta atcactacaa
    26941 tgcacagaca agtaaatata aattctttca tatcttcccc ctttgtgttt tttccttttt
    27001 ttatttgttt tttattgttt ttgcttatgt ttatataata cacctaattt gttaatataa
    27061 agttaagtgt aaattaacag ttcgttaatt tttagaaatt tttgttaaat atattatttt
    27121 tagttaaata cagtctgttt ttaaacaata tattaatttt tatagtatct acattttaat
    27181 ttaaacataa aaaataagtc taacctctat aaagtagaat gttagactta tttttataag
    27241 tatttaatta tacaaccaaa tcattttttt tcttttcttc aagtgctttt ttaagcacca
    27301 tatccttaac cttcataagt ctagtagtac ttgctctctc attttcttca agcttcatag
    27361 ctatatactt aatagtctca atgtaatttg gtatcattac attttcaaga gcattaacac
    27421 gtctacgtgt cttttctatc tcttgtgcaa gaagctgtgc acttttttca ctttctgcaa
    27481 gtcttaaaag tggttgcata gcatctgaga aagcttccat agcagaatct aattcacctg
    27541 atgtaaaagc aagaccataa ggataaatat cactttgatt gttttcagtt ttaaagtcaa
    27601 acacaggaac atcaacactc attatatttc tcgtagaaac atctactgaa actgattgct
    27661 ttggcatcat aagtgctgaa cccagatact cctgactcat aactgctctt gcgattataa
    27721 agtttttata agcagtatca agagcatttt ctgcttcttc acgcaatcgc ttattttctc
    27781 ttacaatctc aagaaattgt ttcataagtt catcaagttt atctttaaga agcttatgac
    27841 ctcttgtagc agttttaagg agtttcttaa gtctagtcat ttccatacgt gttggattta
    27901 tatttaatct agccacagat atcactcctt aggcatatat ttttcaaaat attcatcacg
    27961 gatacgttta agttcacttt ttggtaacat actaagaagc ttccatccga tttctaatgt
    28021 ctgctcaata gttctatcag ttctaaatcc ttgtgaaaca tattctttct caaattcatc
    28081 tgcaaactta gcatacatta aatcaatctc agaaagtgca gattcaccaa gtatagccat
    28141 aagttctttt gcatccttac ctcttgaata agctgcaaaa agctggttca ttgtatctgc
    28201 atggtcttca cgagtcttac ctttaccaat acctttatct tttagacgag aaagtgatgg
    28261 taaaacatct ataggaggct gtatatcttt tttataaagt tcacggctaa gtataatttg
    28321 cccttctgta atgtatcctg taaggtcagg aattggatgt gtcttatcat cctcaggcat
    28381 tgtaagtatt ggaatcatag ttatagaccc ttcatgccct ttcatttttc ctgctctttc
    28441 atacatagta gcaaggtcag tataaagata tcctggatat ccacgacgtc ctggaacttc
    28501 ttttttagct gctgaaactt cacgcaatgc ctctgcatag tttgtaatat ccgttattat
    28561 aacaagtaca tgcatatctt gctcgaaagc aaggtattct gctgctgtaa gtgccattct
    28621 aggtgtagat acacgctcta cagctgggtc atttgcaagg tttacaaaca ctacagaacg
    28681 gtcgattgca ccagttgctt taaaatcgct tataaaaaat tcagcatctt caaaagttat
    28741 accaactgca gcaaatacaa cggcgaactt agaatcagtt ccacgtactt ttgcctgtct
    28801 tgcaatctga actgcaagat tggcatgtgg caaaccagac cctgagaata taggaagttt
    28861 ttgccctcta accaatgtat taagtccatc tatagcagag actccagttt gtataaactc
    28921 ggccggataa tcacgtgctg ctggatttat tggagctcca tttatatcaa gtcttttatc
    28981 tggtataatt tctggacctc catcaatagg tctacccatt ccactaaaaa cacgacctaa
    29041 tatatctggt gaaactccaa agtcaagact ctttcctaaa aaacgaactt tactttcagc
    29101 aaggtttata cctgttgagc tttcgaaaag ctgaacaagt gcagtatctc catttacctc
    29161 tagaactttg caacggcgaa tttctccatt tgaaagttca atttctccaa gttcatcaaa
    29221 ttttacacct tctacgccgt ttattaacat aagaggaccg gctacctctt gtattgactt
    29281 atattccttt atcatagttc ttcagccccc ctctttttaa taaggtctgc aagctcgttg
    29341 tctatttcat cttcaattga atcgtaagct gcatctatat cgccttcttc tacatactta
    29401 gcacgaccaa tcttttcaag aactgaaagc ttgataatat cgtttatagt aacattttgc
    29461 ttgatagcat cttgtgctga cttatagaat tttaatataa gtcccatcat tctatattgt
    29521 ttatgaagtg atgtataagt atcaacttca tggaaagagt tttgatgaag gaagtcttct
    29581 cttatagaac gagtaacctc tagtatcaat ctatctccat ctgaaagtga atcaacacca
    29641 accaattgaa ctatttcttg aagttcagat tcaacttgta aaagtttcat agcttgagct
    29701 ctttgagctg agaaattatc atttacattt ttatctgccc atacatcaac tttttcctga
    29761 tagagtgaat aactagtcaa ccagttaatt gctgggaaat gacgtttata tgctaaagat
    29821 gcatcaagac cccagaatac ttttactata cgaagagttg cttggctgac aggttctgat
    29881 gtatcaccac caggaggaga aactgcacca attgctgtaa gtgttccttc tctttcatcc
    29941 atacctagac aatttacctt tcctgctctc tcatagaatt gagcaagacg tgaacctaag
    30001 tatgcaggat aaccttcttc accaggcatc tcctctaaac gaccactcat ctctctaaga
    30061 gcctcagccc atcttgatgt agagtctgcc ataagtgcaa cactatatcc catatctcta
    30121 aaatattcag ctattgtaat accagtgtat atagaagctt cacgtgcagc aaccggcata
    30181 tctgatgtat ttgctataag cacagttctt tgcataagtg actcgcctgt tcttgggtct
    30241 tttaattcag gaaattcaag aagaacatct gtcatttcat ttccacgctc gccacagcct
    30301 atatatacaa ctatatctgc atctgcccat ttagcaagct gatgctgtgt aacagttttt
    30361 ccacttccaa aaggtcccgg tatagctgct accccacctt ttgtgattgg gaaaaatgta
    30421 tctataactc tttgacctgt aacaagaagt gaatctggtg ttttcttttc tctatatggt
    30481 ctttcttttc taactggcca tttttgcatc atagttacag gtatatcatt acctttttca
    30541 tctgtaataa cacaaactgt atcttcaaca gtaaattcac cagaatatat ttcttttata
    30601 gtacccttaa ctccataagg aaccataatt ttacattcta ctactgctgt ttcttgaacg
    30661 ccaccaatta tatctcctgc tacaacctta tcaccaacag attttttagg tttaaattcc
    30721 cacttagttg ttctatcaag tgaagatact tcaacacctt tagttatatt agtacctgaa
    30781 atatcataca ttttttcaag tggacgttga ataccatcat aaatagttga aataagacct
    30841 ggtccaagtt caacactcat aggcattcca agagaaacta cttcttcccc tggtcctaga
    30901 ccggaagttt cctcataaac ctgtatagaa gctttatctc catgcatttc tataatctcg
    30961 cctataagat gtttatctga aactcttacg acgtcaaaca tattcgcatc tctcatgccc
    31021 tccgctacaa caagcggtcc tgacaccttt acaattgtgc ctgttttcat attctctcac
    31081 ctgcaatcat atactttatc aaaatttaaa tattccctcc tatattttat tagcacatat
    31141 atttagcacc aataaaattt gagattgtct tttattcaac tttcatctat tctttaaata
    31201 atatatctgc acctatagca cgtttactag attctcttac ttcattcata cctaatccca
    31261 tacttcctcc aatacctgga ataactataa tggcaggaag ttggtaatct ttgtattttg
    31321 ctattgattc ttttgcaagc aatgatgcct gttctgtaat ataaattatc gcatattcat
    31381 cttcaaccaa cttatgtata ctttttttaa tttcgtcact atcataggct gggaagatat
    31441 ctattccaag agctaaaaat cccatgatgc tatctttatc tccaactact cctaccttat
    31501 acataagcat cccttagcct ttcttttata gtatctgcgt ctatattatt aagcttacta
    31561 gtcaaaataa ttctaattgt ttttatctct gattctttag catataaata tgcaattagt
    31621 ggttctaatg taagaggctt aaattttgct cctctaacat attccataag atagtcatca
    31681 caaagttttt caaagacagt aaaaccacta tccatgccat ttttacagac atcaaagtaa
    31741 gaagtagatt taaaacatga tgctggagta tctgaggaaa aagcttctaa gaatttttcc
    31801 ttatcaagtg agccacctga aacaaacaca ttcataaaca tatcaaaatc tttcttcata
    31861 tttttgactc ttataaaact ctttagattt gttaaatcac aaactttcat aacataatct
    31921 attacaaatt tgttttggct ttctttagca gtttctttca tactggtaaa ggttgcttta
    31981 tctagaacaa tatcaaccat ctggccattt tgtgtcttat tataaacatc aaatgcctct
    32041 aatatagctg atgacattat cttaggtaaa tcactaaaac tacgatttgc taaagattct
    32101 ctaagcttaa taagaggaat tgtacctcca tcaattaaat atttctcacc atctacacca
    32161 gaaatttctt ctttaatcag cacttttaaa ttgtgatagt catttttata aagaaataca
    32221 tcaacaaatt tttcttcagg agctagactt ttaagaattt catatgtctt tgataattct
    32281 tgagtcaaaa tattctcaaa attatgtata ttattatttg atgtatcagc ataacctgcc
    32341 tcagctataa ttcttaaaga gtcttcaggg cttttggctt ctgccatttg aataaacatt
    32401 tgtctgttaa gaagctgttt ttcaagcact cttattcgtg caacggcata gggataagtt
    32461 ttttcttctg ccatttttta acccctcctt aattaaacaa aatttctgct gcaatttgtt
    32521 caatatattc atgctctact gcaattatgg cttcaaatga ataattgtac tcaatgtcac
    32581 cttttttaac tatgaaacct ccagtaatat ctctagtttc atcagacact ttaatgcctt
    32641 tttttgatag cacatctttt aatgtttttt tatctttttt agataatact atctctgaat
    32701 catctgtaca atttgatttt tcaatcatgt ttagtataat ctcttcatat tcattatctt
    32761 taagatttaa aagtttgttt ttagcttcta aaattacctc ttctaaaatc tcttgttttt
    32821 ttgataatat ttgcttttta gcctccatat aagctcctga tatttcctta gaagccgctt
    32881 tttctgcttc tgcctctgca agctttgtat aggaagccat ttccttttca gctttttcat
    32941 tagctgaatt tacttttaaa tctgcttcac ttttagcttt atccaatatt tcttgtgctt
    33001 cttgtttggc atcagctata attctgtcaa tcattttttg ttcattaccc ataggagtgc
    33061 ttcctccttt tcattttttt tacttatgaa ttataattta taattaacca attgcaattc
    33121 cattgtaagc taagaaagaa acaagtagtc ctaaaagagc atatgtttca actattgctg
    33181 caaatgttat agctttacca agttcttcag gtctttttgc aactattcca actcctgctg
    33241 ctgctgcttt accttgtgct atacctgata ctaaaccaac taaaccaatt ggaagtcctg
    33301 ccataagaag ttgtaaacct tgagctgatg ttggtattgc atctccacca ataatccctg
    33361 cttttgataa aataaggaaa gctatgataa atccatacag accttgtgta ccaggaagaa
    33421 gttgtaaaac tagaagttga ccgaatttac tagggtcttc tgttacaaca cctgctgcgg
    33481 cttgacctgc tattgaagta cctttagcag aacccatacc tgcaaataat gctgctatag
    33541 cagctcctga tattgccaaa aattgaccat ttcctaacgc gtttaaaaat tccatattta
    33601 catctccttt ttattaatgc ttgtatattt tgttttatac ttaaacggaa caaaaggaac
    33661 tcctccgcct tcataaaact tattgaaaaa ttctacatac tgtaacctac ttgtatgaac
    33721 atatgcacct agtgcattta taagaaggtt tattgtgtgt cctacaacac caattacaac
    33781 tgcaagtatc ggaccagcta tagctcctaa aaggttgata acttgtgcta taactcctgt
    33841 tgttaaacaa agcgccataa ttcttgtata agataatata tcagcaaaat aacttgttat
    33901 accatagaga cttgaaaatc ctttaaaaag cttaacagga atacctttaa agcttcttcc
    33961 ttgagtaagt acaagaccta tagcacctat aattgctaaa tattttccta cttctgaaaa
    34021 tacacctatg tcccctgcaa caaaaggtat tataagtaga catacacctg ttatacacaa
    34081 ataccaaaaa cctatgtcaa agattgcatc tattaccttt ccacttctta ttaatgtata
    34141 tgcttttatt cccaaaccta catatatatg gataataccg aacagtaacg acattcccat
    34201 aagtgtcata acatcctcta aaggattaat taatgctttt ataggaatta aatcccccaa
    34261 aatacccccg aatataagac cccatacagt agttgaaact ccacagatac caataagttt
    34321 aattaaattt ccttcacctt tttgtatctt taatttatat acaacaaatc cacaaactac
    34381 tgcaattatt aacccatatg cggcgtcact tagcatcatt ccaaagaata caacaaagaa
    34441 gaaagttaaa attgtatttg ggtcaatatc ttttgtactt ggatagctgt acatgttagt
    34501 tacactttca aaaggtgtta ctaatttatt gttttcaagc agaattggga agccttcttc
    34561 tttacttcct tcttcagtct gaacacaaca atcaaacttt tctgtaattt ctttaattaa
    34621 ttcatctgcc cttttgcttg gtaaccaacc attaagacag aatgttgttt ttgttttgac
    34681 aagtctctct ataatctttt tttggtctct ttctatagtg taaaaatcat ataggttttc
    34741 aagcatgtgt atactatctg catgtctttt aatttcctct gttttgtcct tagaaacgtt
    34801 ttcctgtcta attatcattt cattgtattt cttgatagct tgtaacggtg taccttcttc
    34861 atttggtaat gaaaccactg aaaaattaaa ttcttttaaa acttctaaaa catcatcaaa
    34921 agtttctttt aaagtaatta aataaacata gttcattaac ttgtcagaat tgacaatccc
    34981 aataacactt tctggtattt catcttctaa ttttactctt aaagcatcaa tgtttgcgct
    35041 tatgggcaca gttccaagta tagtttttgt atacttagtt tccatatttt caataggaat
    35101 atcaaagttc acccatggtt ctaacatact tttattgttg ttaagtaagt ttatgttaga
    35161 ccttattgta cctatctcct tgtgcaaatt gtttaactcc agagcttcag cataaacttg
    35221 cccttctttt tcactatcaa ttttgttaaa ctctcttttt ggtgcaagta gggatttttt
    35281 ttgtttcact agtgaagaaa caatattaat tgcttggttt acatgagaca ttttctgctc
    35341 tagcattata acttcagatt cttcactatc ttttacaaga taatttttta attcctcctc
    35401 ctcagtaaga aaggcactat catcaatctg aacaaaccca cggttcataa gtagctttaa
    35461 aatctggctt ttttgagctt ctaagccaac aatagatatt ttgttcatat taactattgc
    35521 catattatct cactaccttt ccaataaccg cttcaatagc cttacttttt ctttcttcag
    35581 cagttttttc aattttcaga cattcacttt tagcattagc taagatttta tcaacctctg
    35641 ctctactgtc ggtgactgcc ttctcaataa gagtttcttc cataagcttt gcttgttttt
    35701 ttacatcagc aaatatgctt tcacatttgt ttacagcatc agctctgata gactctgcat
    35761 cagtaacagc ttttactttg atactgtctg ctgtctgctc tgcctcaaca atgcttttta
    35821 ttatttcaaa agccatccaa atcacctctc ttaccttttt actatgtata taaccaatta
    35881 gattttttta ttctaattag attactacct cattgttaaa gtataaacag catttttata
    35941 taataattaa tatttttcaa cgtttatttc ttaataaaac atattttaga atagtacttg
    36001 cctagattta catccttttt tgacttactt ttagactatg acatgagtat ggtttttaaa
    36061 taccattatt ttcaataatt ataatttaaa gcgatatagt ttgtcaatag cgttattttg
    36121 ttttaaaatt aacgttatgt cgacatttta aatttttatt ttattttttt tataatttta
    36181 atttaattta ttacatataa aagtttttta tattcttttt caacctctaa ccattgaagt
    36241 ttattgtttt ttttgaattt ttagaaatat ctcgcactat tttatctaac ttttataaat
    36301 catcaagcta tatcgatttt tgtcacctta tttttaatct aattttttta tatctagtta
    36361 gaaataaatc aatattttta aagattgata aaacttacac ctagttctaa tagttttatt
    36421 ttaaggcaat gtattaatct taagtagata atatcattag ttactctata tattcgacaa
    36481 cattataaaa aaatcctcgt accaatcact ccaatcttca ataggatatg ctgctcctcc
    36541 atttacactt ttcagtagat tggatgtata ttcttctccc atagaaaaaa gttcccctac
    36601 tgtcatatca taacctctag atgtaacaac cttacaaaat gaatccataa catcacgttg
    36661 ttctcttgtt tttataagtt cctttctaaa ttcatcatca ttcttagctt cttctaaaat
    36721 tttagttaat ttttcattca ttatgtattt ctccttttaa gtagtctttc tgtaaacttc
    36781 aataagcttt atataaaaag gaagccatcc taaataaact aagatgactt cgcttaactg
    36841 ttagcaacac atttactagt ataaaataat tggtgtacct ggttctatat tttcatatat
    36901 tttctttgcc ttagaaaaag gtgtatttac acatccatgt gaacctgaag acttatatat
    36961 acttccacca aaagaatttc tccaagtagc atcatgaatg cctatgttcc cattaaatgg
    37021 aagccaataa cttacaggtg aactgtaacc ttgaccagtt aatgaaacat ttctagcctt
    37081 ataattaaga ggatatatac ctgatggtgt agcaaatcct ctagctaaat cacctgttac
    37141 aacgtctgtt gatacaagca tttctccatt tttaaagaac cacatatgtt ggccagaaag
    37201 actaatctct acatatgtat ttccaatatc atctttatta cgagatacag ctttttgtat
    37261 gtaaataggt tctcttgtaa catttttacc agcttttaca acatcaacta atgcttcagc
    37321 ttctttcttt ttatctatca accaaccata aattccacct gaaattgtaa tatctttacc
    37381 tgtggcagtt ttaaatggtc ttgagtcacc aaaagtactg tattttttac ttaaacctct
    37441 tgtatattct accatagcat cattatcaaa agttatgtca taattctttt ctgagtctac
    37501 ttttaaccat ttattaattt cagaaccatc taaagtttcc ttacggtcac taaaatcata
    37561 tgtaacgact acaccaacat acttatctaa tgtcttaata gcttgtttca acttttctga
    37621 atcctttgtg ttgtttggtg cttcataaac tttactttca tccagagaaa tagaagtatt
    37681 cccagaatta attgagtttt gtactaactt ataaaaatca tcttttttca ctttgtttcc
    37741 aagtacctct ggtactataa cataagcgtt tttagtgcta tcatacttag gataagcact
    37801 cttaggttct acaacatttt tactatcaaa acaagataaa gcattaaacc ttttttctag
    37861 taaatcttta ttatatgaaa gagttatcat attattatac tctttagatt taaatattcc
    37921 accaatccat ccaatagcgc tctgactatc ttttatcttt tgaactttat catctggtgt
    37981 aaattttaaa tcaatatctt ttgcttttat ttgttctttc ttattgtttc tttcctctaa
    38041 gtttaaagta taggaattag tactagatgc taacaaatca tttacttgct caactgtttt
    38101 tcctgaagcc tttacgccat taatagtcgt tccaaagtaa aaatgatttc taaaaaagat
    38161 tgccattcct atataagcta caaacaaaac acctactact atagctatct taaattttag
    38221 atttttttgt ccactttctt gttttacctc ttttccatca atcataatta aatccttcct
    38281 tacattgtaa ttacctatgt accttattgg tttttaaaca aaaccatgta tgccttattt
    38341 tatataccca aattactgta tcattattta tatttttaaa tatatagtta tttctataaa
    38401 accattttat cttttagata aaatgattat tataaatccc ccctttcaaa atcccttaaa
    38461 cacaatctct gctatattaa tatattacca tacttttaat aatcgtggga ctatctatct
    38521 atttttgtaa cactaaacta ctattttaaa aaagaaacta attttgtgtt agtttcttta
    38581 tcttaactta tttttatatt gaattttagc ttaacataac gttgttatat agattctatt
    38641 taattatttt agtttctata tcttttccat ctgcatattc tttaagtaat tccttagcat
    38701 atccaaaata ttcttcaata tcagattctt gactaaaaga aaatatacaa attaatattt
    38761 catttgcatc ttctgttatc attgctcttt cagaatcact agttggactc ccaaatttac
    38821 ccactgaatc agacaatata ggaagatttt ctatatttat taattctttc ccaataccct
    38881 tatactgttc tccttcatct cctacagtaa aacatactgg tgaatgtaat ttgcttacat
    38941 tatatgtacc tactggatat aatgatttta ttgatattaa attgtttatt tctactatat
    39001 tatttattgt ataaagaccc ttcccttgaa taattcttct aaccaatgct tctgaagatg
    39061 ttctatatct acttggattt tttccaagct ctttataagc atttcttgca tctttaattt
    39121 tatctagttt cgtaatgtct tcaactttta gactttgatt tatagtatca caaaactcat
    39181 ttatttcatt aattaatttt tctgagcttt tatttactgc tacttttgct tgaatgcaac
    39241 ccagcttagc attaggccat ctttccttaa gtttgtcatc aatacttata tctatcatac
    39301 ttaaacaact cctatacact aaatttttat taaagttttt atcatctata cattatagta
    39361 ctattctttt gttaataaca tgtttattag caaaataaat ttatttttaa tataattttt
    39421 ataaaataca attatttttt gctttatatt agacaataca ttgataaaat aaaaaaacac
    39481 taattattta gcaatgattt attaaaatca agcattcata atagtgcttt atataaaaat
    39541 caaattttgt tttaaagtaa atctaaaaaa ttcttagctg gcattggtcg tgcaaaataa
    39601 aatccttgaa ctaaatcaca tccaaattgt ttcattaact cagcttgttc tttactctca
    39661 acaccttcta taattacaac taaatctagg gattttgcta gagacactat tgattttgct
    39721 atattgcgac ttctctcatc atgttcaatg ccaccaagaa aagacttatc tagcttcaat
    39781 atatcaattg gcatatctct taaacaacta atagaagaat atcctgtacc aaagtcatcc
    39841 atagaaatag aaaatccata cgtcctcaac tcatttaaaa cattctttaa aatatttaac
    39901 tcattaatag ccacagtttc tgttatctct atttcaatga aatctgaact taaattatac
    39961 atttttattg ttttattgat aaattttaca atatctggtt gatacaactc gaccctagat
    40021 aaatttactg aaactggaac tatcttttta ccttgctttt tccattcata taaatcattg
    40081 caaacccttt caaatacaaa tctcccaatt tttaaaatca acttactttt ctctgctatt
    40141 ggaataaata ctgcgggact tataaaacca tgttctggat gattccatct tattagtgct
    40201 tcactcccta tcatttctcc agttacaagt gaaaacttag gttgataata cacttcaaat
    40261 tgatttttta ccaatgctat ttttatatca tctaaaatca ttgattcctc actaagctta
    40321 ttcctaacat cttcattata aatagcataa tttatatttt tacctttagc aacagaccta
    40381 gccatatttg ctttatttac agccttttga atatctactt caccttcttc aacaaaataa
    40441 atacccacac acagagaaat ttcaaagact atactcaaat tactttcaat attacttcgt
    40501 atattatcaa gttttcttat aattttattt cttccatttt tataatcaca gaaaattgca
    40561 aaataatcac ttgaaagtcg tgcatatacc tcaccttctg ttaattcttc ttttaaagct
    40621 tttgctattg taattaataa attgtcacca aattcatatc caaaagtatc attaatcatc
    40681 ttaaagttat taatatcaaa atatactata atataattta aagatggttt atctaaaaga
    40741 tacttactac attctctata aaatttattt tgatttccta tatatgtaac tttatcttca
    40801 taagccaaac ttaatatttc tttttcatta gattttttta tataaattat ataaaaaata
    40861 attgcaataa atattaatac aatagataac actgcatata cagctctgtt gattatttgt
    40921 tttgaattac taaatacaac agtttctgga attgaaaaaa ttaaattcca gtcttttata
    40981 taattatttt cactattccc attatccata gtggcatatc ctaaatatct tctttcttcc
    41041 aacatattta atacagttgc acctgtttta ttttcttgca attctttttt taatctattt
    41101 acttcttgaa tatcattatc ctgttttaac aatttataaa tattttttga taggctatcc
    41161 ccttgtggat gaaggataat tgtaccatta ttttttacaa cataagatat actattattt
    41221 tcataaaaag atatattaat aagtttcatc agagtattga tattgtaagt acaatataaa
    41281 actccaacta ctttatcatt atttaataat ggtactgaaa tagcaatctt ctttatagac
    41341 ttatttactt catcaacata tggacttgaa acatacttct tacctttaat tgagtttttg
    41401 aaaaattctc tatctttcac tgaaaactta tcacctttgg tattttcagc attaccattt
    41461 atatcaacta ttgccatagt gtaaaatttt tgattttcca tttcatctaa aagatttttc
    41521 attttttcct cattaggtat atctggattt gaagcataat agttagaaaa aatatttaaa
    41581 acatctaaat ctttttcaat ttgtctttgt gctagttgag agccttgtac tgcaacttct
    41641 tttatatgta tttttgcctc tgacataaga agatgttttg tatcttctat atagatatac
    41701 cccattgtca aaaataatga tgttaaaaaa agaattgtaa ttaatgtaaa acttttattt
    41761 ctccctgtca ttcgactcct cccctaatta gtcaatactt catatagata atatatcctt
    41821 tttataccaa ttcatcattt atattgtata taactattct taaatgttac acataaaatc
    41881 aacataataa ccataaaaac attcttttat tattataata acactattta attgaatttt
    41941 aaattaaaga ataaaaacta tccattttct caaacaaaaa aactcttata caataaaact
    42001 ttgttttatg tataagagtt tttattttat ctattttata gcaaacttat cttccataat
    42061 atgaatttaa atacatatct tttatttcat tcataagtgg atatcttgga tttgctcctg
    42121 tacattggtc atcaaatgct tgaattacca tttcatctat tgaatccata aacttagatt
    42181 cttcaacgcc aaactctttt atagatttag gaataccaac tttaagtttt aaatcttcaa
    42241 tagctaccaa taaactttgg aatttctctt catcagaatt tccttttaat cctaaaaatg
    42301 aagctatttc agcatatctc tttaatgctt ctggatattt atattgagag aatgctccca
    42361 ttttctttgg tgcatctgaa gaattaaatt tcataacttc tgttatcaaa agagcatttg
    42421 caacaccatg tggtacatgg tggaaagcac ctaatttatg agccatagag tgacaaacac
    42481 ccaaaaatgc atttgcaaaa gccatacctg ccattgtaga agcatttgcc attttttctc
    42541 tagcttctgg gtcttttgca ccattatcat atgcacgtgg aagatattca aatatactct
    42601 taatagcttg taacgctaat ccatttgtat aatcggttgc aagcatagat acatatgctt
    42661 ctaatgcatg agttaaagca tctataccag aagcagatgt taagcttttt ggcatattca
    42721 tcatcatatc agaatcaact atagccatat ttggcatcag ttcataatct gctaatggat
    42781 attttactcc tgtatcttgg tcagttataa cagcaaatgg tgttacttct gaaccagtac
    42841 ctgctgatgt tggtatagct acaaaattag ctttttcacc cattttaggg aatgtatata
    42901 ccctttttct tatatccata aatctcatag ctaggtcttg gaaatctaca tctggatgct
    42961 catacaatgt ccacattatt tttcctgcat ccattgcact tcctccacct atagatatta
    43021 ttacatctgg attaaattct ctcatagctt tagcgcctat cttagcacat tccaaagttg
    43081 ggtctggttc tacttcaaag aatgtagtgt gcttaatatt catttcatct aataaatcag
    43141 taactgcttt agtatatcca ttattgtaaa ggaatgtatc tgttacaata aaagctctct
    43201 tcttattcat tacatcttta agttctttta atgctactcc taaacaacct tttttgaaat
    43261 aaactttttc tggtgctcta aaccaaagca tattttctct cctctcagct actgttttaa
    43321 tattaagtaa atgcttaact cctacatttt cagatactga atttccaccc caagacccac
    43381 atcctaaagt aagagaagga gctagtttaa aattatataa gtcccctata ccaccttgag
    43441 aagaaggagt attaattaaa attctacaag ttttcatggc tgaagtaaac ttatcaagtt
    43501 tttctctttg atttacatca tcaatatata atgaagatgt atgcccaaat ccaccatctt
    43561 ctactaattt ttctgctttt ctaactgcat catcaaaatc acttgccttg tacattgcaa
    43621 gtactggtga aagtttctca tgagcaaaag cttcttctat ttcaactgat tctacttcac
    43681 ctattaaaac cttagtatct actgggactt caaagcctgc aagttttgct atagtacaag
    43741 ctgtttgccc aactatttta gaatttaatc ctccatttac taaaattata tttcttattt
    43801 tttctgtttc atctttatct aataaataag caccacgctc tttaaattct tttttgactt
    43861 cattgtatat attttctaat actataaccg attgttcaga agcacatatc ataccattgt
    43921 caaaagtctt agatacaaga attgagttta cagcagtttt tatatctgca ctatcatcaa
    43981 ttattgctgg agtattacct gcaccaactc caatagctgg ttttccagat gaatacgctg
    44041 atttaaccat tccaggacct cctgtagcaa gggtcaaatc cacttctttc ataacagttg
    44101 tagttaattc taaagatggc tcatctatcc atcctattat accttcagga gctccagcta
    44161 aaacagcagc ctcaagaaca acttttgcag cctctatagt agaatttttt gctcttggat
    44221 gtggagagaa aattattcca tttctagttt ttaaagctaa aagtgcttta aagatagcag
    44281 tagaagttgg attagttgta ggtacaactg ctgcaataac acctattggc tcagcaattt
    44341 tagtcatacc aaatgcttcg tctttctcta taacaccaca agttttagtt tctttatatg
    44401 cattatatat atactctgag gcataatgat tctttataac cttatcttca gcaattccca
    44461 taccagtttc tttttgtgcc attatagcta gaggaactct ttgcttatta gctgccaaag
    44521 atgctgctaa aaaaatttta tctacctgct cttgactata tgtagcaaat attttttgag
    44581 cttctctgat ttttgctagt tttttaacta agctttcaac gttatttaca gtatctaaac
    44641 caactgacat aacttcattt tcttttttat tttctattat tacttgttct ttatgagcca
    44701 ttttaacaac ctcctgagat agttttttat ttatcatgtt aatttattaa cattatagca
    44761 tttacttttt ctattttcaa gttatttttt taacatttta ttttttatct aatataacac
    44821 catttctact aataatatct agctttacaa atacttaatt taactagata tcactttatt
    44881 ttttacgcaa atcactaatg aaatcatcta aaaacttaag gataacgtta tttttttaac
    44941 gtttatattt ttgtattttt tacaaataaa actttataaa aagaattttt aatatgatac
    45001 tctatcaagt ttaaaaataa aaatacttga aaatgaaaat aaaaacttaa ttaacaagtt
    45061 aaagtaaata tagttaaatg tacaaacatt tacaacatat agtataatta aaaaaatata
    45121 tattcatgat ttttatatac tatgaaataa aatatattta atattatata gataaatttg
    45181 aagtttataa gaaggaggat aagcatgaaa aaattaaaaa caattagcat tttttctttg
    45241 attataagtg taattttaac gattggaggt attggaatag taacttatta tgtagataat
    45301 ttgtttatta gaggactatc ggtatttgtt ttaattatgt cctcttcttt tgtatcaaca
    45361 acagttagat tgatatttga agagtcaaaa cgatataaat tctaatttaa aaattggatt
    45421 aagtaaataa ttttaatata ccaaaaatta cggagattag ctcaaaatag actttttagt
    45481 ccatgaggca tttttttgta tgaaattaaa tatattttca tcatttcaac aagaaaaaga
    45541 ggcttctctc tattttgaga cagcctcttt gctagtattg tatctaatac ataatattat
    45601 aagaaccaat tctattgatg tggacttttt ataacaggct gtagctgttt gttctctaga
    45661 gcaagttcta tagttggaat tattagctca gtatgtgcaa tcatagaatt aggctttgct
    45721 ttgcaattat cttgaccaaa aggtacaaaa tatatatttt tagaattaag gagaatgcca
    45781 atatttttga aattaaagct tagagcatca ttcgttgata ttgatattac tagtggttta
    45841 tcattacgaa gatgaccttt tgctgccatt aatactggtg aatctgtaat accatttgct
    45901 aattttgcag cagtatttcc agtacaagga gctattatta taatatcagc aattccttta
    45961 ggtccaaatg gctctgcctc ttcaatcgta actataggtt tatttcctgt aagttcatat
    46021 gcttttttca taaactcttc agaatttcca aatctacaat cgatattttg tgatacatct
    46081 gaaaatatag tgtgtacatt agctccttct ttaaccaaat tctctaactc tataaatatt
    46141 ttatcataag tacaaaacga accagtaaat cctactccaa ttgtaagtcc ttcaagtctc
    46201 atttctaatc ccttctttca tttataatat tttctatagc gttaacaaga atttcaccag
    46261 atgtctttgg tgaatattta ccaggaagcc caaggcaaag agtcgcatta atccctgctt
    46321 ctttaacaac agaataatct actcctccag gagaagaagc tatatctata atagttactt
    46381 cttgtgatac tttttctaac ctttctttcg ttaaaataca ctctggtatg gtgttaaaaa
    46441 tataatcaaa ttgtaaaagt ttttcatcaa gttgacttaa agaaatactg gaatatccaa
    46501 atgcattagc cgtacttctt gcatcattat ttcttgcgcc aacacataca tttgcatcca
    46561 aagtgtataa tttttttgca agtatttgtg cacatcttcc ataacctaat actaaacagt
    46621 tactttgatg tatattgatt atacttcttt taatagcttc tgctatagcc ccttcagctg
    46681 tagcaactgc atttaaaata gcaacatcat tcattttcat aaaatcataa atctgaatag
    46741 agtgttgctc gcaaaattta caaactttag ttggtatgtt acctccaaat aatatttgat
    46801 taggagataa attaattagc aagttatcaa tagtagcatc aagacaatca tccgtatttt
    46861 caatattaac ttgattcttt gtaaatggaa ttggacataa aataatggaa cttttggata
    46921 gtgcttcagc tagtgaagtt gattttccaa cgattccatt tataatttca tttgatatcc
    46981 cataagtgac tacagaatac cctttattaa ttagtttttt taccatgtac acttgtcgca
    47041 ggtctccccc aataacagtt atatcgtatt gatttgacat attgtaagcc tcctttacta
    47101 tatacaatat gtttttatca atacattggt tcactaaaat tactaaatat tatacttata
    47161 acatttttaa taatgctaat ctatctatct tttcagaaga ggtatatgga aatgaattta
    47221 aaaagataat tttctttggg atttcatttt tcattagatt atctttcatt ttaatcaaca
    47281 aatctttctt cgtaattttg tctatagtta ttacaaataa tacaatttga tttcctctta
    47341 tagtgtctga gtacggcaat acaattgcat cttcaatttg aggaattttt ttaacttcat
    47401 tttcaatctt agtagaacta actttaaatc ctccaatatt taaaatgtca tcacttcttc
    47461 cttcaaatat taagtatcca tcattatcat aatatccaat atcattgaca gaataaggtt
    47521 tggttgcatt atatactgca tattctgtat tcacaaaaat ttttccttct ctaatatata
    47581 tatctattcc tggaaatggt ctgcctacac ttagaggttt ttcaattagt tcatcatagc
    47641 ataaatatgt aatataattt aattcacttg ccccatagta taaaataatt tcagactttg
    47701 gcatatattt ttttagattt cttgcagtat cttcaaataa taattgagac cctgtaaaaa
    47761 tacttacaac tttgagtact ggttctttta agtgtttaac caaaagttga agttttgtag
    47821 gtataaggta tatgttagtt acattatatt gttttattgt ccttatccat gatttacaat
    47881 taagacctga acttattaca atactacctc cttcatataa tactgacatt atagaattta
    47941 gattccctgt aaaactaagt gttccattaa caaacaagat tgattcccca gagatattaa
    48001 aaataccatt ttgtatagga aaaaatccag cccaactctc atatgttcta tataatacct
    48061 ttggaacact tgtagaacct gaagataaag ctcccataca aatactttta tctaaataat
    48121 ttataagctt cttatattga tacatatata tattaaattt aggattaaaa atagaaaaat
    48181 taactttttt tgctacttca ttattgttat catcatataa atcaataacg tccatagatt
    48241 catcagatat aatgattgaa atattatttt ttaataaaat atcatctaat acttttttag
    48301 aaagattata atgacaaata ataggaatat tttttgaata attaatagca aaaaaactaa
    48361 tcaactgaaa cattatattt ttagaatata ttaaaattgg gatattttca cctaaagaaa
    48421 tttgttttcc tagttcctca ctataactca atagattttc ataagtatat ttttctccat
    48481 ctataattaa aaaattttta tttttatgcc tctctttttt tattcttaaa agttcataat
    48541 agttcataat ctacaccctt tcaagcagta aagctgaacc tagaccacca gctgcagcta
    48601 tagagcaaat tccatatcta cccttagtct cttctaatgc ttttaaaaga tgtaatgcaa
    48661 taattgcacc tgaagcacca tatggatgtc catacgctaa agctccacca aatatattat
    48721 atctatctat taactctgga tactttcttt gaaacaacac atctataact gcaaatgctt
    48781 cattaaattc aacagcagat acatctaaat aattaaggct ttctatctct aaaagtttat
    48841 ccattgcttg tatcgctgat gttggactta aggttgcatc tgttcctatc gtgcatgtgt
    48901 taataatttt tgctttaggc ttctttttta catgttctaa atacttctta gaacatatta
    48961 taataaatga agctccatca ttaataagac atgaatttgc tgcatttgta atagtttctt
    49021 ttcctaaaat tgatggcatc ctatctaata acctttgact catcttatca cgtatacctt
    49081 catcataggt actattattt ataggagata taatatcttc aagtattttt tcctctcttg
    49141 cttcttttgc tcttttatga ctttccttta cccaaaaatc caagtctgct ttctcaattt
    49201 gatataactc tgctactctt tccgcacctt cgagcatact attttggcta ttatcatcag
    49261 gagaaaattg agcaactgta taatttggat tatttgtatt atatcgctta tcattcttat
    49321 gatatgtgcg cattggttgt agagaactac tttcaaaacc acctgcaata attaaatcac
    49381 actgaccaga tttaaccttt gaaaaagcta tgtctatact catcatagca gaagcacact
    49441 gcatatccac agtaaaagca ggtacttcat ttgacactcc tgctgttaaa gtcattaatc
    49501 tagctatatt ccctcctgtc ccaactgcat ttccacatat aatctcatca attttgtcaa
    49561 tttcatactt tttaattaaa tcttttaaaa cacttgctcc tagtaattca ggttgaacaa
    49621 attgaaaaat tccatttttt agacctatat gacttctcaa tcctcctaat ataaaaactt
    49681 ccttcataca ttacaccctt ccttatatta tagatttatc atagatttct tctgagtaca
    49741 ttgttcagtt tagtgcctaa aaaagatgca ataactgact ttattgtatc tccaattaaa
    49801 aatggtacta ctgctgcaaa aagtgctgct tctactgtca tttgattaaa atagcacata
    49861 aatactgttc ccataaaata tataataggc attccaacaa aaattgttat tagtatatat
    49921 ctcttaatat cattgtttcg tccttttaat aaactaataa ttagtgctgc aaacaaaaac
    49981 ccaaaataaa aaccacctgt tgggcttaac aactttccaa taccagatgt acctccacca
    50041 aatactggaa tcccaattaa acctatcatt atatatacac caactgtact aaatgcttgt
    50101 cttggtgtta aaatcaaagc tattaaattt atcattattg tttgtgctgt tactcctgct
    50161 ggagtaaatg gcaatggtat atatatgtaa gatgatatac acaataatgc tacacatatt
    50221 gacattttag ttaaatcttg aatatttact ttagtatttt tcatttttat aattcccttc
    50281 atatgtctta tttcatttag tttatatgta catatacaaa gcttctatta aagctttgtt
    50341 ttagtataaa ataaagacgt ttttttgtca actattataa gtatttaggt ttacatttaa
    50401 tttataagta aactttatac tgttacttta tactttaata gtcatttata aaactttgct
    50461 taaaaacagc tctatttaga ctatacttgt aaattataat atgatatatt tttatataag
    50521 attttatttt atataaaaaa tagtaacaaa tagtttatac acaagatatt taaaaggaag
    50581 tgatgatatg aaagttaatg aaaataatat aaataatata gatgaagagt tagatgaact
    50641 tagtaggaaa gtttacttag agtcaccaga tttttggata agtatttctg acaaaataat
    50701 agataaagta tttgatgaaa tgttattatt ttttgcagtt aaatataact atttcaatat
    50761 agtatgcttt gctattgaaa acaattatct ggatttaaac atgccttcaa aaaacaaaaa
    50821 atacactaat ataatggagc atttgttaga tacatcaaaa cagaatgaag ataaaaaaat
    50881 ttataattat cttctaagct taaataaagg taataaccta gatgaaagac caattttaga
    50941 tgaattaaac tgtaatatta gtagtgatac ttttgaaaaa aaagatatat atcttccaaa
    51001 gtacttatgt ccaagttgta attctaatat atttgataca ggttataaag tatcaaatga
    51061 cataacatat aaattttctt ctaaagaagc tgaacctatt gaaatgtgta gtgaagtttc
    51121 ttctatcaga tgttgtaatt gtgatgaatt attagaagat attacacttg ataaactata
    51181 taacctatgc aaaattcaaa actgtaataa ttgtggttct aatttaacta aggtaggaat
    51241 tattgcaaag aaaaaaatga actttgatga gaatactgaa aaatttaaag atgtctcaac
    51301 atcttactgt tgtggaaatt gtgactctga aataaactta tctcaaagag aacattttaa
    51361 gatttaaata tttataggct aaagtaataa gattacatta ttagatactg tataagtttt
    51421 ttaaatctgt aaaaataaat acacttatac gaattaaaat tgctatgaaa ctagttcttg
    51481 atgaactaat ttcacggcaa ttttctataa tctaataatt attaaatttt aacaaacctt
    51541 tattagcatt ccataattat ttaaaattct ttcaaatacc ctatagttta taggtgaaag
    51601 tatctttaat attatatcaa acttattatc tttaaatagt aaatttgtta aatctgaaat
    51661 acaccataca attttatttt cctcttttgt agctatatca attctgatta acttacatct
    51721 ataataaata tctttatctt catatagtct attttaaaaa ttactcttaa aaaatcaaat
    51781 ggcaaataaa ttaattatcc ttttaataga atgtaatcat attatttact atattccatt
    51841 atgtactctt taaatcctga atcttcattt acttgttctt ttactatatt aaaacctttt
    51901 ttattataaa acacaactgc ttttttatta tctttatata ctgctaagct tagactttta
    51961 tattttttag ttgcataatc taaaagttta cctccgatac ctagattttg gtatttgggc
    52021 tttataaata aagctcctat aaagctttta tctattatgc ttataaatcc ttttatttca
    52081 tctccatcat catatacaaa tgtatctgat ataggtatat attcgttttt aacagtatta
    52141 tagttatttt gccagtattc tttacttata aagtcatgtg ctttgattgt acttttttcc
    52201 catatttcca tgattttatt tatgtcttta ttgtttaatt tctttatcat aaattttatt
    52261 tcacctcatt tttgtattga ttttataatg tttataggta agtgtatttt atttagatat
    52321 gatttagtta atataaggtg gatgttttag tatatgctat tgttgattct acaattttat
    52381 atactgcttt attaatataa aataagtttc taaatactcg ttgatattgc taatattcta
    52441 cttatctacc cttagttgat ataaaacctg acactgtgcc ttgtgtgagt cttttcttta
    52501 tcctatttac ataccactta gttaatataa aacgttgtta gataaatcat atgctttagt
    52561 tgctttagat ttacatacca cttagttaat ataaaacact tcgaaattgc agttttcagc
    52621 atgttattgc gcaatttaca taccacttag ttaatataaa acaaattcga ggtcttgatt
    52681 ttgaagtttt tggttatgca tttacatacc acttagttaa tataaaacgg tttctaccaa
    52741 tcaaatcacg cataattata gaatttacat accacttagt taatataaaa caatcatttt
    52801 gtatgattgt tccatttttt ttgaaaacat ttacatacca cttagttaat ataaaacctc
    52861 tgtgactcca tatttttttg caatatataa ttcatttaca taccacttag ttaatataaa
    52921 accatttgta aatctttatg attatgtaat atattcataa tttacatacc acttagttaa
    52981 tataaaactt tttgaatgtt gaagattttg ttaatgcagg gaaaatttac ataccactta
    53041 gttaatataa aaccccaaaa taggtataat tgaatacatt gaaaatactt caatcaagca
    53101 attataattc aataaaaaca tcaaaatttg cagtgagcga gcagtagtgc aattgataac
    53161 agttatcata caccttcaat cccttatatt ccaactgtta acacctattt tagcataaat
    53221 atcgctcact gcaaacctat ataaacattt catcagaata cttatcaatc ccataacatt
    53281 tctttttaat tgaattagga ttttttatct cataaacata gattgaatcc tcgctattat
    53341 caataatatt ttctatttca gaaatacatt tatgtaactt tccttcagta atattgcctt
    53401 caaaaacaga attttgcgtc caagtcaaat attttctaag tatccttcta attctattta
    53461 acgaccttgc ttcaacaata tcataagtaa caataacaaa catacttacc accacacctt
    53521 taaaactttg tacctttcat catttataaa atgtttaatt aatttataac actctaacct
    53581 tataaacatt ctatatgaaa cttttctatt tagatttcta tgttttatag ttgtctgcat
    53641 cttattttca aattcttgca aaaatttctt ctttcctttt tcacttaaat aacaaatttt
    53701 gtcttgatat tcaaaatcat tcttattaat tcttttatta ttaatcaggc taaaaattat
    53761 tgtgtcaact ataattggct taaaaatttc tgcaatatct aaacttaaag aaaatctctt
    53821 tgttgatggt tcatgtaaaa agcttattgt agggtcaagt tgtgtcttat atatctcact
    53881 taaaacattc gtatacatta tgctatttcc aaaagacata agagcattta ctgggtcttt
    53941 gggaggtctt ttttctcttt tataaaattc aaaatcatct tttaatattt gattaaatga
    54001 tttatagtat gtttttctta ttctgccttc tttacccata agttctgata tttctttgac
    54061 atcattcaaa tcatttagtt catcctttat agtatcaatt aaactttcat caactttgta
    54121 atatctcata tttcttaaaa tatggtatga tgcactttcc acaaaggatt ttgctaagta
    54181 taatctttca tttttatcta aatagcaggc tgattgacat actaaactga atcctgatac
    54241 atttttcttc cttggataat atgtacctga ataatatcca tagtaattgt agaaatgaag
    54301 catcacccca tatttactta tgtaatttat aagttttgtg tttaaatcta cttctccata
    54361 tatatgaata tcatcaactt gttctactgg taatattttt ttctcacctt ctaaattgta
    54421 atagtaaatc gtattttctt tcctttttat atccccatta cttataatat aatagtcttt
    54481 atacattgtt actcctcctt cacaaaacaa aattcataat aagcacattt tgtacaataa
    54541 tgttttttct caattttggg aggagataac tacatattta tttcatttat atttataagt
    54601 gtgttttcaa taattctttc tttttcttct gtcaatatca attcttcttg tttcttttcc
    54661 ttaacataat ttatagttcc ttttttctct atacccatac tattctttaa atagtataag
    54721 taatagtata actgcatttc atctgcttct ttcatctttg aacttatttt tacttctttt
    54781 acatactcat cctttattat atctagcttc aagatatcat caatcaatat ttctttattt
    54841 ttcattcttt tatatgaatc ttcatgtact atcttcccag acaatactct actactattt
    54901 tgttccatgg ttattccttt agaaaatagc catagttttc ttttacatac ataataataa
    54961 tttattttta caccttgaac ttttaatact tctaaatcta ttggcacaaa atcaccttct
    55021 ttagtaaatt ttagccacat tgttaatata aaaatcaaat aaattggtct atttcattac
    55081 ttataagcac acctttacca aatatctgtt ttgtgtgctt attttcgtgt atatcatatt
    55141 tatataaaag tatttttaaa tcttcaagac ctttcacctg tatgtcaatt acattttgtt
    55201 tagctttata tattggtaaa tttacagttt ttttaattat ctctcttctt gctttttttc
    55261 tcttagactt tattttattt atcaattgtc tatcatcatt actatatgca tttataagct
    55321 cttgtcccaa ctcttcataa tctttgatta aagtttcttc aattttatta tatatatcct
    55381 ctggaattac tgtatatcct tcaatatttc taagtatatt ttgtgcatct ttagaaccta
    55441 aactatatgg cgttatagta tctaaaatat ttaaagcact ggtaaatctc ttttcaaaag
    55501 ctgtaccttc tagactttct ttagagtata gtatcttaac catttctacc ttatattttt
    55561 ctttcatctt cacacattct tttccattta taaaagtttt cagtagctca agtccttttt
    55621 ctacaatatc cttatcataa atactaccta ttcctgtagc tttttctgta tatataaata
    55681 tatttgggct attttcttca tactcacgac ttctataaca tctaccaaat cgttgaaata
    55741 gactgtcaag tgttgaattt tctgtatgaa gctcatcaaa atcaatatca agggatgctt
    55801 ccactaattg tgtagtaatc caaataccat tactatcact atctgcaaat tcttttatat
    55861 atttttctaa ttttgctcta tcttcttgta tatacataga atgtaaaaga tttaagttga
    55921 catctattcc ttttggttta actatttctt ctattaactc atatttttct acagcacttt
    55981 taactgtatt tactattaca agaacttttt tattcattcc actttgtatt attttaccta
    56041 agttttcatc tattgaattt tctacaatag acacacagtg tcttattttt tctgtgttac
    56101 atgtcaagtc agctaagtta ctattcatta cacctctttt ttttaattca tctatatata
    56161 tggttggcat agtagctgtc attatcataa atctgccacc tatcttatgt atcatttcta
    56221 taccttttac caatacagct gctatttctg gtgaatatgc ttgtatctca tctattacta
    56281 cttttgaata tgctaatgtt gagtacactt tttcatagcc tctatacaaa aaaggaaatt
    56341 taaatatttg gtctattgtg gaaaatgtca gtttacaaga taataacttt gctaaatcta
    56401 caatctcact tgaattttct tgattacttt cttctagata atctattgct gttgaatgta
    56461 acaaacctaa gaatgtatca tttgattctc caactccaac tatatttttt gctctatcaa
    56521 ataatgcatt tatacttact cttaatggaa gtgtaaaaaa tgccttgtct ttatctatcc
    56581 aaattaaggc agtttctgtt tttcccattc ctgtagatgc aatcagtatt atattcttat
    56641 ttctattaga tttagcaaat gattgagcct ctctcaaact accaaactct ttcattaaat
    56701 aattttctgt ctgttctcct atatttataa cattattgca ttccacaact tcatgagcag
    56761 aagcactatg gtctagtcta tgtagtattc cttttaacat aatatataaa ttatagtact
    56821 tatgattttt atctattctt ttttctacac tttgtagata tactttactc aatctttctg
    56881 tttttattgg atatctaact ttaaattcat gctgtagttc ataaacttta tttattaaat
    56941 cttcatctaa tattttttgt attaaatttt taaaatcttt atctataaag atatctcttt
    57001 catgatgata tacaataact tgatttaata ctgctctaag ttctttattt tttttcctgt
    57061 ctatataact ataatcaata aatgcaggag aaagataatt atggcctaca ttattttcta
    57121 aatgagttac tattttaggt tcatttattt tagtatccat tctgcttttt attaactctt
    57181 gaaacggtga aaatgccttt ccaatatcat gaaattctat aacaaagtca agtaattgcc
    57241 aaaatatctc ctcttctaga aaatctaagc tatttatatt ttttccataa ctttctctta
    57301 atacattcat ttgttttaaa agttcatcag tatgctctct aagtgtttcc actggattag
    57361 atttagcata taacatactc tccctcctaa tttatattat taaaatataa taaatctcct
    57421 tcattatcta ataatatatc tccaattgat atagtatttc catcctcaac ataataacta
    57481 tctaccttat tccaaaccct ttttttatct tccacagcat tattatcata gtaattattg
    57541 agcctataac ttataccatc tatatctgta tcttgtaaac ttgtttttgg tatatatata
    57601 ggcattctta tattacattc tgataactta taattttcat aatcatctaa ttgtatatcc
    57661 tcatctactt caaatttatt gacttctaca aatctaatat cattaattct tactaaatct
    57721 tcttttctac ctaatgataa atgctcgtca taatttaata aactatcata tatttcttcc
    57781 aataaatcaa aatccccacc tatatgaatt atcaaattta cattcaatag catatgacta
    57841 ttcattggca tagatgtaat actatctttt ttataaaaat acgttgtctg gtaattatta
    57901 aatatacttt catatgttcc ctgtacactt atattaaacg gtatatattc tgtagcattt
    57961 aatactttgt gaattaatcc atttattgta gaatatggag gcagtggata tgtctctgta
    58021 atcttcatag caaatggttt tttatagcat gcagtttctt gaaataaatc taatttcaaa
    58081 accttcatat taattcaccc cataatattc ctttacttct ttaactagtt gtccaaaaaa
    58141 tttatctaca cttaaaaaat tatcttcaaa caatccctta aattcttctt tatttataaa
    58201 taccccatct accattccaa cataggtaaa ttcttttaaa tcttttccta aaaaagtacc
    58261 ttgaattact tcttctatag cttgcttatt tattttaaat ccatttttat ctccttttaa
    58321 tttaattcta cccaagaaaa atggatttgc tatttcatat actcctccta ctacaaatag
    58381 aggagataag ttttcttgtc tacctctaat atttctattc aatactttta ttatttctaa
    58441 aaattctact actcttctac atttctcttt attatcaagt tctatatctc catccttacc
    58501 tattttagat aaatctatag taaccgtata tgtatagtaa cttaaatgtt gctctatgtt
    58561 ggcaagatta gggtgttctc ctattctatc tgcaaatcct ttattattta aaaaatccat
    58621 atcacttcta tatggctcaa gagaaatcgc attacttaat cttacagtag cttctctagt
    58681 caaagaacct ccttttttat ctttctcatc ttttttattt gttttcatat atccaaataa
    58741 atccatttct tctgattctc ttatacttat attatcttta aattgaatag tacctttttc
    58801 tttatcgaca acctgtaaat tccaattaaa caattctgct gctaatcgta ctatgtcata
    58861 tcttaaacac tggcgagatg caaatgtata catatttcca tcagctctac ttaatttttt
    58921 taattctgat atattgccta tcccttcacc atagtttaaa ctttgagcct gtgctattac
    58981 tgtaaatgtt aatcctcttt tcatcttatt tttcctcctt ttcgtttggt agtttatctt
    59041 ttccttcata ttttccagaa attaatccag atataaaagc atgacctata gattcaaagt
    59101 ctaaatcttt ttcaatttct atatctaaaa taaatgctgg aatctgtttt tcagctgaca
    59161 taaaaactct tataattgta tccataaaat cacttttatt tccgacttta actgaattta
    59221 aaagtttata gcttactcca tctattttat ttttagaatt tttattaaca aaatagtcat
    59281 gtatatcaca acctaaataa taaattattt tcagcttttc atcattcttc tccaaatttt
    59341 tactatccat tttatatacc ccctttttgt agctatttat taatactctt aattggacta
    59401 ctctaaaaca atctattcct gatatatttg atttgttgtt gctttccata tcatttttaa
    59461 ctttatttct taaaatgtta ttagttagat gcttcaagtc cctatttttc aagagtaaat
    59521 ctagtacatt tgatttaagc ttgtaatcat agatactttg tattttttta tttgcatact
    59581 tgtctgtaaa aaattttgct aaataagttg gaatattaaa ataatttatt ttacattttt
    59641 tagaatctat acttgcttta aattccacaa aaagtatatt acgaagttgc cattcacttt
    59701 ttaatgtatt ttcttctact atacttttta taagctgatt aaaaggattt tcatctttgg
    59761 aatctgaaga ctcacttttt tgagctttta aactattatt tctttcaaaa agctcattta
    59821 tagaagtatc catgttaaca aataaataaa actcattttc ttcattaatt aaataattct
    59881 ttcttgtata agtagcccct gctggagtac agaacaatat tagcttacaa acatcacata
    59941 ttgatgatac atactcctga ttccaaaaca tattcctagc attattggtg cttactccaa
    60001 gtggagcaaa attactttct gagtattcat ctaatattcc cttatataaa ccacacattt
    60061 cacatacttc taaactatcc atatactctt ttatatcttc tatgctttta tttttcttta
    60121 taaagttttt atttatttcc ttcataattt tttctatagt atttatacta gacttactaa
    60181 cttttttttc atttatatcc ttagctatac tatttaatct atcatttaaa tagtttttca
    60241 acttatcata gtcattctct tctaataaat cttctaactc tccaaaatat ataatttgtc
    60301 ttaaataatc attaaacatt acctgtttta atccatctaa atctaatttc gccttagcaa
    60361 cattaaaaaa actaacttgt ccaaaataat tatcttgaac tacatattta taaagatttg
    60421 ctgtaagcct gtcatttatt gattttattt taaatatatc tatagtctca tctactaaag
    60481 attctaaact atcaaattca tcataacttt ttatcttaga catactatct agcttctctt
    60541 ttatcaggtt aaatttttct tcatcaaatt ttttaatctt atcattttgt tgtttcacac
    60601 tatctttaat tttcttaatt ccatctttga ttctatcagg cttagattta ataaaattta
    60661 tactataatc aatatttttt ttaattcttt ttgatacatc atattcatcc ataaagtaat
    60721 taaaataata ctcatgaaag ttttcaagaa ataagctatc aaattcgata taatcttcca
    60781 taataaatat ttcagcttgt ttatctgctt tctttattat atttaaaaat ccaactatac
    60841 ccatattaaa aaaccaatct cctctataaa ctcttaactt catttatctc acctccattc
    60901 taaaatatct acatctccaa atccctgagc acgtctaaaa ccaattccta gattgtagat
    60961 aagatttaaa tcatcaatat cacctttcaa tataaattta cctttgtaac cattaatata
    61021 ttgatactct ttacctgtta tttttttaaa ttctcttaaa ctctctttaa ctactacctt
    61081 tttgagacct atattttcaa attctaactt tctttttaat ccattccccc tataattttt
    61141 aaccacttca ttagttatat agtttagttc ttctatgtat ctatcatcag ttatctccaa
    61201 gaattttcct tctttagact ttacacagat tggagacaat gcattaaata gtaccctttc
    61261 ttctgttacc ttcttttctc ttgataaatc gattctaatt cttgtaagtt cataggcttt
    61321 atataaacat tttttagaag tcattaaccc attgtatata tgaaaaccca attctaaatc
    61381 tggagtactt atatttaata ttactttatc ttctataata aaattgtcac cttcaatact
    61441 atactttttt atataaaccg aaaatgtaaa atttttagtt tttttattat tcttttcttt
    61501 ataatagtac atgtttttat agtaatcttc attagatttt cttattgctt cttttataat
    61561 actcataaag agcgaattat aagatattgg tatacaacct gtactaaatt caattttcat
    61621 tttcataata tcacctctct caattaatat attaacacta gaccgtgaag tatattggtc
    61681 caagtataaa ttatttgaaa aaatcagtaa atttttttca ttttttcaat ctctgacaat
    61741 atatcatttc taagtctatc tggttctaca acctcaacat aagctcccat acttaaaatc
    61801 caacttttta tctcctcata acctctcatt ttagctttaa acattataga tttatcatca
    61861 tattctatga tttgttgatt attaacccaa actttctcct ttataattgt tgaaaatgga
    61921 tgactaattt ttataacaac attaatttct tctcccttat aaattcctat actattttta
    61981 ctatattcat cccaactaaa acttttatct acattatatt tttcttctaa aaccttataa
    62041 tctttaatcc tacataattt aaagtctata aacttaaatc ttttttcaca aaaagctacc
    62101 atataagtat ctgatttata attaaataaa ccatatggat gaacaattct ttcactattt
    62161 cctgaattta atgaataata ttctatccaa aatttgtgtt ttgtggtata tgctctaatt
    62221 atttcattac acttattttt ttcagactca taatcacaat tacgttgtgc ttgaactgta
    62281 aaataatcca tatatgtatc gcttttttct cctgtattta atactgcttt tattttttct
    62341 acaatattat taaattcatt tttgtaaata tcattattat actctaactg tgaatttatc
    62401 atatctagaa ttgaaacttc actatctaat aatttaatta atgatatgct attacattta
    62461 tctatttcat aaccaccata aatcccaggt gttgaattta taaatatacc tgcttgctct
    62521 aaatacatct tgtaactttt tatttgcctt tcacttactt ccaactcttc tgataattct
    62581 ttgcatttca ttttgcctct gctttgaatt aatatcatca ttctaagtgt attagctaat
    62641 ttactaatga gtaacccccc ctaataacat taattgatat ttttattatt tatttctaca
    62701 catatgtaca tataacctct ttatatttta tataagtaga atttttttat aattttgaca
    62761 tttaccatat tatattttat atttgtacaa aattttaata gttaatacaa taaaaaatat
    62821 tactgaaaaa ataaacatct ataattccag taatattaat taattttaaa ccctttttat
    62881 tatcatttaa taatagtaac taaaaattga ctaaatttaa ccttatctct tttaaataat
    62941 tatttcttac aattaaaaaa taaactattt gaaccaagaa caagcttccc attaccaaaa
    63001 cagctgatga tgctacttct atatcaaaag aatttttcag tgcagtaagt gcaaatattg
    63061 aatgtataac agccactaca taaggaaaca aaaatactat tccaatctca attgttgaag
    63121 cttttttaat ctcattatat gtcataccca atttatttaa ctgttcatac tttttcttat
    63181 caacttggca atccatataa aatttattat ataaaaaact acttgtagta acaaagaaaa
    63241 ttaaacctat aaatattgct aaaaatataa atgtagaata cattaatttc ccagttccta
    63301 tcattaattc tttagagaaa aacaaatatg gttgcatccc tgactcatta ttaaatttat
    63361 tttcaaattc tctgcatatc tctatagttt tacttgaatc ctctacttca aatgcggtaa
    63421 atctatccac tataaatttt gattctatac tatcataaaa attatccttt accacataca
    63481 tatttttgta atatgctggc ataacacatt cttccacttg ttttgccacc tttaaacctc
    63541 tattccctac aataatattt tctttgacgt tattatttcc tggtaaactt gatgataata
    63601 atgatataca ttcattatct tcaaaactta taggcttaac ccctatagtt tctgtaattt
    63661 ctaagtactt agattctttc ataattctga cattaaaatc acttttcttg ggaaatacag
    63721 ttttaaattc accattagtc cttgtatagt ctacatcctc tttttttaga ttattttcta
    63781 taaacatcat tctttccttg tctttttctt tgctatcagg ctttttaggg tcattatgca
    63841 atgtcattgt tgaataatat attgtgttag gatatattaa attaatttga cgctctacat
    63901 ctttccaaaa ggaataaact gtacctatag cagtaaatgc tactgcagaa gttatagtta
    63961 tcaagaaaaa tattctagca ttatccttta ccttataaat caagttcgaa atccaaagca
    64021 tatttgtatt ttttctataa aatctcttgt ttaattttag cttcttcata aaaaatacac
    64081 taaattgaga aaagaacaag taagttgcta ctatagtaag ggatgtaact ggaactaagc
    64141 gcagatatgt tgtatattgg tcggttgagg taatagcatt ataatacgct attataaaca
    64201 aacaaatact taatatagca agcattagtg atgttttagg ctctggtttt ggagtttttg
    64261 tacctttaag taacttcaac acctgatttt ctttaactat aaaagaagta aatattgatg
    64321 ttaatacagc taaaatcata aaatagatta aagtagacaa tatcgcatta aatggtatat
    64381 agaattttaa tggtgataat tctaaaaaag tagacataac cactaaaaat atctttgcaa
    64441 agattaagcc tattattatt ccaattacag aagatactat atttattatc atattttcta
    64501 taaagattaa tttcattact tggcgctttg atatacctgt aatataaagt attccaaatt
    64561 ctttatatct acttttaaga aacacactaa ctgaataaaa cacaaagaag aataagaata
    64621 gatatgcaat tatcttactc atcttgagac cttcttttaa ataatcagga aattgagata
    64681 catctaaatc tggatgactt gtaaacataa taaaagaaaa tagcagtgaa gatgatataa
    64741 caatacttgc taagtatcct aaatacgctt taatatttct tgaaatattg ttaatagcaa
    64801 actgttttat attcattagt tatcgcctcc caataatgta agtacatcca ttatctcttg
    64861 ataaaactgc tctctactac tacccttata tatttcatta tatatatacc catcttttat
    64921 gaataagatt ctactacagt agctcgctgc taatgggtca tgagttacca tcatagtagt
    64981 aactttattt tctttattta tcttttgaaa tagctccatt acaacctttg atgactttga
    65041 atctaaatta cctgttggtt catctgctaa aagtattgat ggattgttaa tcaatgctct
    65101 agcaatagca gtcctctgag cttgaccacc tgatacttca aaagttcttt ttcctaataa
    65161 atcttttata cctaatattg tagatacttc attcaatctc tcatcttgct cttttacact
    65221 gaccttatct aatgtcaatg gcaatactat attttctcct atagttaatg tatccaataa
    65281 attaaaatcc tgaaatacaa aacctaactc tcttcttcta aataatgcaa gttcctcacc
    65341 tcttaacaat aaaggattct tgccctttaa ttccatagtt cctgtagttg gcttgtcaat
    65401 agtagaaatc atatttaaaa gagtagtttt tccactacca gatggaccca taattcctac
    65461 aaattctcca tcttctatac taaaatttat gtcatttaat gcgttgaaaa ttattttttt
    65521 accatatact ttacttaaat tatttacagt taaaattttc attatttaac tatccttcct
    65581 aatttaattt ttgaaataac atatatataa ttgttaatta taatttatct tagtttaatt
    65641 tttatagata ataaatctat tttttctata cactaattat gttagtaaaa gctaatactg
    65701 caagtactac acccatgatt attctgtata cagcaaacac tctcattggt tttttcttta
    65761 aaaaattcac gaatttttcc ataactatta ttgatacaat aaaagctact ataaacccaa
    65821 ctaccaaagc aatccacaat gttggtgtca ttattgaata atcaaattca aataaatctt
    65881 ttcctgaaga agccaccata gctggtactg ctaaaaagaa agaaaactca gctgctgtag
    65941 gagaattaag tcctgctatc catccaccca taatagtaga tgcactcctt gacatacctg
    66001 gccatgcaga caaaacttgt aatgtcccaa cctttaaaac ttgtattgga gtaattttat
    66061 ctatattatc tgtggaatga ttcttttttc taaacaaagt ttctactact aataataaaa
    66121 tcccacccac tataaatcct ataattacag attgtaaatt aaacaatgat tttatattat
    66181 cgtaaaatag aactcctata atcgcaaatg gtatacaacc tataattaca ttcattccaa
    66241 atctaaaacc aatctttcct tcttttccgc cagtaaatat aaatttaaaa aattctataa
    66301 cgctatcttt tatctttttc caataaagta ctactactgc taatatagcc ccaagttgta
    66361 ttacaacctc aaacatttca gcaaattgcc ccttaaaatc tattaaatta ccaaccaaaa
    66421 tcatatgacc tgtagatgaa actggaataa attctgtaag cccctctact atagctatta
    66481 tgactgattt caatataaaa ataacatcta aactcatcaa taaacatcct tcctttttta
    66541 caaaatttct ttttttatgt tatttctctt atgttatttc ttatattaat tgtaatttag
    66601 attagaaaat ttaactatct gcaaacatta cacaaacctt acatctatgt aaggtttgca
    66661 aataaactct cttaaatttt gattatttaa ctatgaatat ttcatttaat aaattgtata
    66721 ataaaaaagt acaattaact aaaaacccta ttgtaacaga taatttacac ctttattaaa
    66781 ctatttacat ctttattaaa gtagattgaa acttctgtac cttttcctac ttctgatttt
    66841 atacttactt tatgacctaa atcatcacat acctttttta ctagataaag acccatacct
    66901 gtagattctc caaacttacg accattttct cctgtaaaaa atgggtcaaa tacccttttt
    66961 aaatctttag aaacaattcc aattccttca tctattatac ttaaaattac atatttatta
    67021 ttttcttcag attttatagt taggtagtta ctataatctt tagaatattt cactccattt
    67081 gtaattaatt gacctattat aaatttcatc cattttacat ctgtataaac ataagtaaat
    67141 ttgtctactt gaatcttagg aatcattcca ctttttataa ataacctttt ttcttcattt
    67201 acaatactag atacaatttc atttaatttg gctttttcaa ctataaagtc tttctgaaaa
    67261 ttatctgccc ttgcaaaatg catagccata tctaaccctt tatttaatct atctatttct
    67321 tttcttatat tttcagttac ttcttctccc tcataatctt gaagctgaag gcttataact
    67381 gatattggtg ttttcatctg atgaatccat tggttgatga aagttaaatg ttcactatgt
    67441 atctttttat gctcttgtat cattgattga tacaatttat gttgttgttt taaaataact
    67501 gatatatttc tccccaagaa tgagcttcct aaatacaaaa atgactcctc caaagaatgt
    67561 actccttttt caaacagatt atatacttct ctatttttat aatacttata tattaaaaaa
    67621 cataacaata taaaagaact aaataaaaga atatataaac tctctccaaa actaataaac
    67681 ttcataagat tacagtaaat aattgtcata actatgccta tataatatat aaaaatataa
    67741 cctttataat cttttaaaaa taacttcata ctttctcctt accaattctt atttaattta
    67801 tatcctaacc ctcggacagt ctgaacagca ttatctatac ctaattcctc aaatcttttc
    67861 cttatcctgc taacatttac atttaaagta ttttcctcta caaactcaat atcatcccat
    67921 atcttttcta aaagataatc tctacttact acttttgggt atctcttgat aagacattca
    67981 atcagtatac cttctttttt agttataatt agattctcac catttaattc tagttctaac
    68041 ctttctggat aaaactttaa tccatctagc tccactattc tttcgtctat tttaggtgaa
    68101 tattcaccat atacccttct tatatgactc tttatttttg ccattactac atcatagtaa
    68161 aaaggttttg ttatataatc atctgctcca ctttctaaag ctctaatttg gtctacatta
    68221 ccatctctag ctgatataaa aattataggt aaattcgact gttgcctaat acatctacac
    68281 cagtagaaac catcaaattt aggtaaattt acatctaata gaactaagtg tggcttaaaa
    68341 ctttcaaatg taatcattat atcatcaaag ttttcttcaa caatcacttc aaatccatat
    68401 ttctcaatat aatctttaag taatgtactt attgatatat catcttccac aatatatact
    68461 ttataactca tataattcct cctaatatac ctatttactc attat
//
LOCUS       DAENCV010000003        64750 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAENCV010000003 DAENCV010000000
VERSION     DAENCV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.003"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            305..1138
                     /locus_tag="KQD82_000147"
     CDS             305..1138
                     /locus_tag="KQD82_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG4378653.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1231..2163
                     /locus_tag="KQD82_000148"
     CDS             1231..2163
                     /locus_tag="KQD82_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG4378654.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2215..2697
                     /locus_tag="KQD82_000149"
     CDS             2215..2697
                     /locus_tag="KQD82_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4378655.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2709..3578
                     /locus_tag="KQD82_000150"
     CDS             2709..3578
                     /locus_tag="KQD82_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG4378656.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3621..4376
                     /locus_tag="KQD82_000151"
     CDS             3621..4376
                     /locus_tag="KQD82_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG4378657.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4418..5239
                     /locus_tag="KQD82_000152"
     CDS             4418..5239
                     /locus_tag="KQD82_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG4378658.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSGT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5244..6005
                     /locus_tag="KQD82_000153"
     CDS             5244..6005
                     /locus_tag="KQD82_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG4378659.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6166..6423
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6536..6775
                     /locus_tag="KQD82_000154"
     CDS             6536..6775
                     /locus_tag="KQD82_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG4378660.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7077..8072
                     /gene="ilvC"
                     /locus_tag="KQD82_000155"
     CDS             7077..8072
                     /gene="ilvC"
                     /locus_tag="KQD82_000155"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG4378661.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8125..9813
                     /gene="ilvB"
                     /locus_tag="KQD82_000156"
     CDS             8125..9813
                     /gene="ilvB"
                     /locus_tag="KQD82_000156"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG4378662.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVASMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10023..10700
                     /locus_tag="KQD82_000157"
     CDS             10023..10700
                     /locus_tag="KQD82_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG4378663.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10874..12910
                     /locus_tag="KQD82_000158"
     CDS             10874..12910
                     /locus_tag="KQD82_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG4378664.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13101..13667)
                     /locus_tag="KQD82_000159"
     CDS             complement(13101..13667)
                     /locus_tag="KQD82_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4378665.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            13955..14890
                     /locus_tag="KQD82_000160"
     CDS             13955..14890
                     /locus_tag="KQD82_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4378666.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINHEI
                     KKYGRRFKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGNGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15356..16570
                     /locus_tag="KQD82_000161"
     CDS             15356..16570
                     /locus_tag="KQD82_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378667.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16989..17510)
                     /locus_tag="KQD82_000162"
     CDS             complement(16989..17510)
                     /locus_tag="KQD82_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4378668.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYNKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17866..18663
                     /locus_tag="KQD82_000163"
     CDS             17866..18663
                     /locus_tag="KQD82_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG4378669.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18773..19363
                     /locus_tag="KQD82_000164"
     CDS             18773..19363
                     /locus_tag="KQD82_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG4378670.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNKIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19429..21126
                     /locus_tag="KQD82_000165"
     CDS             19429..21126
                     /locus_tag="KQD82_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG4378671.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21340..22179
                     /locus_tag="KQD82_000166"
     CDS             21340..22179
                     /locus_tag="KQD82_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG4378672.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            22696..24552
                     /locus_tag="KQD82_000167"
     CDS             22696..24552
                     /locus_tag="KQD82_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG4378673.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    24635..24875
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    24941..25176
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25261..26478
                     /locus_tag="KQD82_000168"
     CDS             25261..26478
                     /locus_tag="KQD82_000168"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG4378674.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            26595..27077
                     /gene="cdeM"
                     /locus_tag="KQD82_000169"
     CDS             26595..27077
                     /gene="cdeM"
                     /locus_tag="KQD82_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG4378675.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            27342..28607
                     /gene="hisD"
                     /locus_tag="KQD82_000170"
     CDS             27342..28607
                     /gene="hisD"
                     /locus_tag="KQD82_000170"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG4378676.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(28810..29562)
                     /locus_tag="KQD82_000171"
     CDS             complement(28810..29562)
                     /locus_tag="KQD82_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4378677.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(29680..29949)
                     /locus_tag="KQD82_000172"
     CDS             complement(29680..29949)
                     /locus_tag="KQD82_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378678.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            30424..31518
                     /locus_tag="KQD82_000173"
     CDS             30424..31518
                     /locus_tag="KQD82_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG4378679.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKREKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            31520..32470
                     /locus_tag="KQD82_000174"
     CDS             31520..32470
                     /locus_tag="KQD82_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG4378680.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRAKHG"
     gene            32463..33647
                     /locus_tag="KQD82_000175"
     CDS             32463..33647
                     /locus_tag="KQD82_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378681.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRITKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGNVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            33660..35156
                     /locus_tag="KQD82_000176"
     CDS             33660..35156
                     /locus_tag="KQD82_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG4378682.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSTSIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            35179..36129
                     /locus_tag="KQD82_000177"
     CDS             35179..36129
                     /locus_tag="KQD82_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378683.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            36150..37112
                     /locus_tag="KQD82_000178"
     CDS             36150..37112
                     /locus_tag="KQD82_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG4378684.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIAKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            37484..38116
                     /locus_tag="KQD82_000179"
     CDS             37484..38116
                     /locus_tag="KQD82_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4378685.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            38527..38619
                     /gene="kdpF"
                     /locus_tag="KQD82_000180"
     CDS             38527..38619
                     /gene="kdpF"
                     /locus_tag="KQD82_000180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG4378686.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            38660..40342
                     /gene="kdpA"
                     /locus_tag="KQD82_000181"
     CDS             38660..40342
                     /gene="kdpA"
                     /locus_tag="KQD82_000181"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG4378687.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            40367..42424
                     /gene="kdpB"
                     /locus_tag="KQD82_000182"
     CDS             40367..42424
                     /gene="kdpB"
                     /locus_tag="KQD82_000182"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG4378688.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            42443..43069
                     /locus_tag="KQD82_000183"
     CDS             42443..43069
                     /locus_tag="KQD82_000183"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG4378689.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            43306..44214
                     /gene="cysK"
                     /locus_tag="KQD82_000184"
     CDS             43306..44214
                     /gene="cysK"
                     /locus_tag="KQD82_000184"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG4378690.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            44241..44831
                     /gene="cysE"
                     /locus_tag="KQD82_000185"
     CDS             44241..44831
                     /gene="cysE"
                     /locus_tag="KQD82_000185"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG4378691.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            44991..45179
                     /locus_tag="KQD82_000186"
     CDS             44991..45179
                     /locus_tag="KQD82_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG4378692.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(45233..46102)
                     /locus_tag="KQD82_000187"
     CDS             complement(45233..46102)
                     /locus_tag="KQD82_000187"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG4378693.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(46299..47543)
                     /locus_tag="KQD82_000188"
     CDS             complement(46299..47543)
                     /locus_tag="KQD82_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378694.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLGEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            47834..48145
                     /locus_tag="KQD82_000189"
     CDS             47834..48145
                     /locus_tag="KQD82_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG4378695.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKKIEQL
                     T"
     regulatory      48238..48330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            48430..49224
                     /gene="thiD"
                     /locus_tag="KQD82_000190"
     CDS             48430..49224
                     /gene="thiD"
                     /locus_tag="KQD82_000190"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG4378696.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            49248..50036
                     /gene="thiM"
                     /locus_tag="KQD82_000191"
     CDS             49248..50036
                     /gene="thiM"
                     /locus_tag="KQD82_000191"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG4378697.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            50059..50691
                     /gene="thiE"
                     /locus_tag="KQD82_000192"
     CDS             50059..50691
                     /gene="thiE"
                     /locus_tag="KQD82_000192"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG4378698.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            50830..51132
                     /locus_tag="KQD82_000193"
     CDS             50830..51132
                     /locus_tag="KQD82_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4378699.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(51198..51947)
                     /locus_tag="KQD82_000194"
     CDS             complement(51198..51947)
                     /locus_tag="KQD82_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378700.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPFILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(51941..52903)
                     /locus_tag="KQD82_000195"
     CDS             complement(51941..52903)
                     /locus_tag="KQD82_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378701.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNSSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(52907..53365)
                     /locus_tag="KQD82_000196"
     CDS             complement(52907..53365)
                     /locus_tag="KQD82_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG4378702.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            53721..54077
                     /locus_tag="KQD82_000197"
     CDS             53721..54077
                     /locus_tag="KQD82_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4378703.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            54149..54865
                     /locus_tag="KQD82_000198"
     CDS             54149..54865
                     /locus_tag="KQD82_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378704.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            54858..55682
                     /locus_tag="KQD82_000199"
     CDS             54858..55682
                     /locus_tag="KQD82_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4378705.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            55694..56893
                     /locus_tag="KQD82_000200"
     CDS             55694..56893
                     /locus_tag="KQD82_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378706.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            56902..57714
                     /locus_tag="KQD82_000201"
     CDS             56902..57714
                     /locus_tag="KQD82_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378707.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            57725..58993
                     /locus_tag="KQD82_000202"
     CDS             57725..58993
                     /locus_tag="KQD82_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378708.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPDISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGVVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            59359..60540
                     /locus_tag="KQD82_000203"
     CDS             59359..60540
                     /locus_tag="KQD82_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG4378709.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(60682..61599)
                     /locus_tag="KQD82_000204"
     CDS             complement(60682..61599)
                     /locus_tag="KQD82_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378710.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            61852..62646
                     /locus_tag="KQD82_000205"
     CDS             61852..62646
                     /locus_tag="KQD82_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4378711.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIELARKEDI"
     gene            62647..64707
                     /locus_tag="KQD82_000206"
     CDS             62647..64707
                     /locus_tag="KQD82_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4378712.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLILLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSILLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT        25639 a         6718 c        11100 g        21293 t
ORIGIN      
        1 ttttgctatt tattatttgt ttaatatttc taatttataa aaaaaaaatt ataaatttta
       61 aaaattataa atgacatttt aactagaatt aattgtaaaa aacactatat aatactcaat
      121 tttcaacatt ctatacaatt atacagaaat tatacagcat aataatgacc gaaataacaa
      181 attttaaaaa ttaagcaaaa ctttttgtcg aagttcaacg tctatatata tgtagtgtaa
      241 atttttggga taaaatacta caaaaatata aatgtaaaaa tatagtaata ttggaggtcg
      301 aacgatgaga aataaaaaga aaaaaataaa tagaaagaag ttgtatttgc ttttaagtgc
      361 tattgtagtg tgtctggctt ttatagtagt tggtgttcgc gtcagctctt taaatatgaa
      421 ggaaaatgaa attagtagaa atgcaaaact gtcaagttct actattacag atatagtatc
      481 taatacatca atagaagtta gtaaagggcc aagtaaaagt tctggaaaaa tagcatacat
      541 aactatagat gatggtccat ctaaatttac tgacgagatg ataaaaactt taaataaata
      601 caatgttaaa gcaacttttt ttatgataga tgggaatatg aaggaatatc cacagcaagt
      661 taaaaatata attaaaaatg ggaacacagc aggttttcat agtgtatcac atgatattca
      721 caagctttat gttactagta catcagcaaa agaggaattt gatacaaatg atcaaacttt
      781 ttataagata actggtaaac attcaaaagt aataagaata ccttatggaa gcaaaccata
      841 tacaccacaa gcctcttatc aggctttagt tgatgctgga tataaaattt gggactggga
      901 cttagataca gaagactgga gatctaattc ttctcaaatt gtacaaaatg taaaaaatca
      961 tattaagaat agaaaaggtg aagataaaga ccaacttgta gttttaatgc atgagaaaaa
     1021 acagagtgca gaagctttag attcagtttt aaaattctta tcagatgaag gttatgaatt
     1081 tgcgccagta gatcaaaacc aaataccaaa aaattattgg ttacgtaatt tagagtagtt
     1141 aaaatataag aatattgatt gatattttta gaaattgaga ataaaaatga gtgaggtgta
     1201 ggtatattga ataaaaaaat ttatatagga atggtcggaa tattgtcttt gatgatggtt
     1261 ggttgtaata aaagtttagc aaaagtaaat gatgtggaaa tcacaaaaga acagtacaaa
     1321 aaaacaaaag cagttttatc tgctactaat aattatataa atgggcagtc tttagatgaa
     1381 ttagaaaaaa ctttggataa aaaaggtaga aataaattag aaaatgttat aatatcgttt
     1441 atggtggata atgaactttt atatcaagag gctaaggata aaggactaac tccaagtaaa
     1501 agtgaagtag actcaaaata tcaagaacta gaagataaga tgaatttgaa tacaagctat
     1561 aaggaaaaaa tggacaaagc aggtgttgat aaagattatt taaaacaaga aatatctaga
     1621 gatttagcaa tagataaaaa taaaaaggcc tttgaagata gaataaacat aagtgataat
     1681 gatatggaag cctactatac aagtcataaa aaagatttta atgtagaaga agtaagtgct
     1741 tctcagatac ttatatcgac tttagataaa aataaaaaag aagtaagcaa agataaaaaa
     1801 gaagctttga agaagaaggc agataatata ttgactaaaa tcaaaaatgg agaaagtttt
     1861 gaaagtttgg ctaaaaaata ttcggatgat aaagcaactg gaaaaaatgg aggacagcta
     1921 ggatatttta caaaggatga taaaaatgct gagtttacaa aggaagtgtt caaattaaag
     1981 aaaaatgaag tttcaaatgt gtttgaaact agttatggat atcatatagt caaagttact
     2041 gataaaagag aaagacaaaa aagttttaat gagtgtcaga gtttaataag agaatctatt
     2101 ttaaatgaaa agtatattga gcatataaaa aaactgaatg aagatgctaa aatagacaga
     2161 tagaatttct aacagctcaa tcctagcctg taatcggagg gagattttac aggaatgttt
     2221 aagagtttaa agagaacgaa agttgagaaa tacattatcg ataataaaga ctctttttat
     2281 agaatagcat atagttatac gaagaatgaa gaagatgcct tagatgtggt tcaagaagcc
     2341 atgtataagg cactatactc agtggaaaat attaaagaag taaattatat aaagacatgg
     2401 ttttataaaa ttttagttag aacgagtata gattttatta gaaaaaaccg taaatataat
     2461 aatatgacag atattgacct tatagatgag actggagagt atgataaata tactgatttg
     2521 gatttaagaa gagctttgga agaattacct attgaataca aatctattat aatattaaga
     2581 ttttttgaag atttaaagat tgaagaagta gcaataatat tggatgaaaa tgtgaatact
     2641 gttaaaacta ggttatatac tgctttaaaa aaactaaaat taaaaattga agagtaggga
     2701 taaaaagaat gacaagcaaa gaaaggttaa ataagttaaa agatgagtat cataaaattc
     2761 caatacccaa gaaattagat actataataa atcatgaaaa aatagaaaac atatatcgag
     2821 agaaaaataa aaaagtaaat agattaagat ttaaagttgc aatagccttt gcatgtatat
     2881 ttacagtatt agtaaatata agtcctgtat ttgcagacaa tttttctaag atacctgtaa
     2941 ttggagcaat agtagaagta ataactataa aaaattatag tttaaaaagt gaaaattatg
     3001 aagctgagat tgatattcct aaaatacgag gtttaaaaga taaaaactta gaacaaagat
     3061 taaacagtag ttttatggaa gatggaaaaa ggctttatca tcaattccaa gagagaatgg
     3121 agaagataca atcatcaaag aataaaggat ataaatcttt aagtttgagt tatagtgtaa
     3181 aaaataattc taaaaaattt ctatcaatag agatgacaaa aaatgagata gaggcttcat
     3241 catatgtgag taaggtacat tatactattg ataaaaaaag acaaatagtt ttgactttgc
     3301 caatgctttt taaagatgat aagtatataa aagttataag tgataatata aaagaacaaa
     3361 tgagagaaca aatgaaaaag gatagtacaa aatcctattt tatagatcaa aaaaaagact
     3421 tgcctgttga agattttaaa acaattaata agtatcagga tttttatttt aataaaaatg
     3481 aagaccttgt aatttgtttt gatgaatatg aagtagcacc tggttatatg ggcgcagttg
     3541 agtttgtaat accatataaa gtaataaaag atttgtaaat tgaatagtag tttatatata
     3601 aatcttttaa ggaggacaat atgactacat ataaaaaact taaaaaatct atatttaaaa
     3661 ttgctatatt ttcaatatgc ttattagttt cattagtaga tacaaaccct atatttgcag
     3721 gtagttcctt taatgaaact tcaaatagtg atacgataaa ggtgttaaat gctaagagta
     3781 tcaactttaa acaagacaat tatgaagtga atgtgagtat tcctaaaata aaaggtttaa
     3841 aagataaaac tttagagaat aaattaaaca atgagctttt gcaagatggg aaaaaaattt
     3901 acagtgaatt tcaaaataaa atgaaaaagg aaaataataa gggtcataga tacctaaatg
     3961 taatccataa tgtcaaaaat aattctaagg actttttatc aatagaaatg acaaaagaag
     4021 agatagaagc ttcctcatct ataactaaga agcattacac tgttgataaa aatagaggaa
     4081 tactattaaa tttaccaata ttttttaaag atagtagata tattagagct ataagtgata
     4141 atataaaaga gcagatgaga aatcaaatta agaaagatga tacaaaatct tattttatag
     4201 accaagataa agactcgcca gtgaaagatt ttgaatctat aaataagtat caagattttt
     4261 attttaataa aaatggaaat cttgtgatta gttttgatga atatgaagta gctcctggtt
     4321 atatgggagc agtagaattt gtgataccaa ataaagtaat aaaaagacta agatagatat
     4381 atagaagatt tttagataga ctggtaagga gaattatatg aatatatatg aacaagcaat
     4441 aagattaata gaaaataatg aggatttcgc atttgctaca ataacgtctc attcaggttc
     4501 aacaccacgt gaaactggag caatgatgat tgtgaaaaat gattctacta tatttggctc
     4561 agtaggagga ggtagtgtag aagcagaatg tataaaacat gcaattaatg ttataaaaaa
     4621 cagagaatca atgttgtata aatttactct aaacaaatct gatgtggcaa aactaggaat
     4681 gatatgtgga ggtactggag aaatacaaat agattttata gatagtaaat taaaaagtaa
     4741 tatagaaaaa tttaataaga gactaaagga aaatacaagt aaagcatata tatttggtgc
     4801 aggtcatatt tcaagagatg ttgctgtgat actatctctt ttagaattta gaacagtagt
     4861 aatagatgat agagaagaat ttgctaatca tgaaaggttt ccagaatcag aagtagtagt
     4921 tttagattct tttgaaaaca taccagattt tccaactgac gaaaatagtt atataattat
     4981 tttaacaaga ggtcatctat atgattcaag tgctttagaa tgggctctta aaagagaggc
     5041 tggttatata ggtatgattg gaagtggaac aaaaattgga ttgacttacg aaaaactcat
     5101 gaaaaaaggt tttaagaagg aagaattatc aaaagtacat gcaccaatag gcataaaatt
     5161 gaatgctcaa acaccagctg aaatagctgt atgtatagct gctgaactta taaattgtag
     5221 agctaataag gaaaaataaa ctaatgaaca cagggagtaa agatattgat actttaaagg
     5281 ttaattttag agggattgaa ataaaagcag ttttagtata taaaaataga aaaaatattt
     5341 ctataaaaat agatccattt ggcaaaataa ctgtaatgag tcctcctaaa atatccaaaa
     5401 aaataataaa agatataatt attgaaaaag gagactggat acttaaaaag tcagaaaaat
     5461 gtaaaggaag agaggaagta tataaacaaa gaatgtttat aactggcgaa aaatttcttt
     5521 atttaggtga agagtaccat ctagttataa aagaactgtt gagagattct gtcaaaaaaa
     5581 gtaactgtag aataactatt aacgaatatc aaatagttgt tcagacaaat gatacaagta
     5641 cagattttat taaaaaatct ttaaaatctt ggtataagat ggaaagtgaa agaatagtat
     5701 tagagagaat agattttctt aaattaaatt gtgaaataat gaagcaatta gttccagcga
     5761 gtgttaaggt aaaagaacaa aataagagat ggggaagttg tacagctcaa aaaaatattt
     5821 atataaactc aaaaatatct atggcaagat tagatgtaat tgattatgta ataatacatg
     5881 agtttagcca tttagtacat atgaatcact ctaaaaaatt ctatgattta gtaaaatcca
     5941 ttatgcccga ttataaagat aaagaaaatt ggctaaaaat aaatggatat aaaataataa
     6001 tctaaaaacg attattccaa aatataggcg taaagtataa aagaagtatt attgtgaaaa
     6061 aaataaataa tctgaaaatt ttaaaaaaag tcattgacat tgtaaatgat agggaatata
     6121 atctatatta acaacaaaac agaattaaaa tataaaatta aaaaattaaa agcaatgaca
     6181 gagacaacaa tactaatagt gttatccaca gagaaccaat gttgctgaga attggtgtta
     6241 acagtagtat aatgatcact ctggagcttt tgtactgaat atttttagta agtacaaacg
     6301 tatgtcagcg ttacatgact aaggttaagg taattaatta cccgacctga aaaaagaggg
     6361 gtagatgtat tttttatatc tacaaataag gtggtaccgc ggaatataac tttcgtcctt
     6421 ataagtaata tttatattgc ttataaagat gaaagttttt ttattttaag cgatataaat
     6481 aaatttaatc catataaaat taataaaaaa ataataaaaa taaggaggga tttttatgga
     6541 aaatactgta tatgctaaat taaaccaagg aatagattct tttttaagag tggctatgac
     6601 tctaagaaga agagaggttg atatacaatc aataagtatg actgtagata atatgaataa
     6661 ttcaggtata aaattaatag taaatgaaga aaagacatct ttagattctg tcttaaacca
     6721 tatgaaaaaa ctacatgata taagagagat aactgttgaa agaatgagac attaattaaa
     6781 tatattttat aaaactaaag ataaatatgc ttattttatt aaaaaactaa agataaatat
     6841 taaataagaa aaatcttaga ttaaaatata agtgtcattg aatttattag tttatcacag
     6901 ttaataaatt tattagtttg ttgtagctaa taaaaactga aaattctaaa attataattg
     6961 taagattata taaatttaga atttaaatct gttgaataat attaaaacaa tgataatgag
     7021 tatgaatttc taataaaaat aaatatcata aaaataagaa aaagtattgg gaggaaatga
     7081 aaatggcaag aatgtattat gaaaaagatg tggatttaga ggttttaaaa aataaaaagg
     7141 ttgcagtttt aggttatgga agtcaaggac atgcacatgc acaaaatctt agagataacg
     7201 gtgtacatgt aatgataggt ctttatgacg gaagtaaatc agcacaaaaa gcaaaagaag
     7261 atggatttga agttaagagt gtagcagaag ctacaaaaga aagtgatttg acaatgatgt
     7321 taatgcctga tgaaaaacaa aagaaagttt atgaagagag tgtaaaggat aacttaaaag
     7381 aaggtcagac attagctttt gctcatggat tcaatataca ttataatcaa gtacaaccac
     7441 cagaatttgt tgatgttgta atggtagctc ctaaaggacc tggtcactta gttagaaatg
     7501 tatttacaaa gggaagtgga gttcctgcac tatttgcagt atatcaagac tacactaaaa
     7561 aagctacaga gacagtttta gcatatgcaa aagggatagg agcaacaaga gcaggtgttt
     7621 tagaaactac ttttaaagaa gaaacagaaa cagatttatt tggagaacaa tcagtacttt
     7681 gtggtggaat aagtgaactt ataaaattgg gatataaaac gcttgtagat gcaggttatc
     7741 aaaaagaagt agcatatttt gaatgtttac atgaaatgaa actaatagtc gaccttatat
     7801 atgaaggtgg atttgaaaga atgagatata gtataagtga cacagcagaa tatggagatt
     7861 atgtatctgg taaaagagta ataactgatg ctgcaaaaca aggtatgcaa aatgttttag
     7921 aggatataca aaatggaaaa tttgcaaaag cgtggattaa agaaaatgaa gaaggacgtg
     7981 aaaacttcct aaaaactaga gaagaagaat ataatacaga aattgccgaa gttggtagaa
     8041 atctaagaag tatgatgtca ttcttaaaat aaatatatat taaatggatt agtttgaggg
     8101 aaagagtggt gtatagtagt gcgtatgaat ggagcaaagg taattttaga atgtttaaag
     8161 aaagagggaa tagacacaat atttggatat cctggtggag ccgtgatacc tctttatgat
     8221 gctctatacg attattcaga tgattttaaa catataagga catcccatga acaaggttta
     8281 gttcatgctg cagatgggta cgcaagaagt actaatacag taggagtttg ttttacaaca
     8341 tctggtccag gagcaacaaa tgccataact ggaatagcaa cagcttttat ggattcttca
     8401 cctatggttg taatttcagg gcaggtgcca actagtttat taggaaaaga ttcttttcaa
     8461 gaaattgaca taactggagc aacattatct atgacaaaac ataattattt agttagaaac
     8521 actaaagaat tagttcctac aataaaagaa gcttttagag ttgcaaattc aggtagaaaa
     8581 ggacctgtac ttgtagatgt tccaaaagat ttatttttag cagaaatgga ttttagtggt
     8641 gaggactatg atttatgtca aatagatgat tatatggatt ataagagtga ttttgatcta
     8701 gatgatgaaa ctaatattaa gcttttaaat gaagcaatag atatcataaa agaatctaaa
     8761 aagcctgtaa tatatgcagg tggaggtgtt aaatcttcag acagtgagga aattcttgaa
     8821 aaatttgcaa ctaagataga tacacccgta ctaaatacac ttatggggct tggaaatata
     8881 gatagaaaga atgaactatc tcttggaatg gttggaatgc atggaagtag agaaagtaat
     8941 ttagcacttt ctaattcaga tttagtgata gcgataggtg cgagatttag tgatagagtg
     9001 ataagtaaaa gttctgaatt tgctaaaaat gcaaaaataa tacacataga catagaccca
     9061 tctgaaataa gtaaaaatat agaatctaat gtatctttgg ttggagatgt aaagctagtc
     9121 cttagtttat taattgaaag ggttgaaagt aagaataata gcaattggaa agaagagata
     9181 aagacattta gaaagagtga gggtgtacaa acatatgaat ttcatccaca aaatatactt
     9241 aaaaaaataa atgaaaaata tgaaacatta aagaaaccca ctgtggtagt aactgatgtc
     9301 ggtcaacatc aaatgtgggc tgctaagtac tggaatttta aaggtaataa aagttttata
     9361 acatcagcag ggttaggaac tatgggattt ggtttaggtg cagctattgg aactaaagtt
     9421 ggtaatgtag ataaaaatgt agttttagtc actggtgatg gaagttttag aatgaactgt
     9481 aatgaactag ctacagttgc aaattataat gtacctatgc ttattttact actcaataat
     9541 agaacattag gaatggtaag gcaatggcaa aagttatttt ccaatcaaag atactctcaa
     9601 actgacatta atgaaaatgt ggattatgtt aaactcgtaa atgcatataa tattgatgga
     9661 tataaggtag ccagtatgga agaattagga aaggcgctag atatgataga ttttaataaa
     9721 cctgtatttt tgcaatgtga tatagataag gattatgatg tttatcctat tgtagcacct
     9781 aatgatgcat tggaaaattt gatatgtaac taaaacttat actgaatatt gataaaattt
     9841 tttattaaat ttttattggt attcagtttt gttgtttaga gatagattgg cattttttat
     9901 tttttcaaat aaatcaatga ctatataatt gagaacttat taaattttaa ttatacaatt
     9961 tggaatagta ttacaactta agtaaataat aattttgaat aataatttac ggaggtattc
    10021 atatgtttaa acacgataaa gcattattaa aagaagtaaa agtagaaaga ccaaatcctc
    10081 aatatgctgt tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta aaagcagcta
    10141 tgcaatactt gtcccaaagt tttagaataa aagaccctca aataaaagat ttatttcttg
    10201 atatagcagc agaagagctt agccatatgg aaatggtagc acaaactata aatttattaa
    10261 atggacatga tgttgattat aattcagtta atacaggcga aatagaaaca catgtattaa
    10321 ctggtctatc accagtctta ataaactcat caggagctcc ttggactgct aattacgtta
    10381 cagttacagg ggatttagta gctgacttac tttcaaatat agcttctgaa caaagagcta
    10441 aggtagttta tgagtacttg tatagacaaa tagatgataa atatgtaaaa gaaacaatag
    10501 atttcttact taatagagaa gaagcacata atgctctatt tagagatgct ttaaataaag
    10561 ttaaagatac aggttcaaat agagattttg gagttacaga agattctaaa ttatatttcg
    10621 atttgtctac tccagggcca aatcacgata ctaagataga tattaatcct ccttcttttg
    10681 aaaaaccttt aaaaaaataa tttgatattt tttatgttgt tttgttttag tagttataaa
    10741 aattatttac tagaagaatt aattatatga aaaattaaat atgtatagtt aaaaatgggt
    10801 atacacttat tataagtgta tatctatttg tttattgcaa aacatataat aaaataacaa
    10861 atgaggtggt attttggaca gtaatagaaa acctaataat ctaataaatg aaaaatctcc
    10921 atatctttta cagcatgctt ataatcctat aaactggtat agttggaatg atgaggcttt
    10981 taaaaaagca aaagaagagg ataaaccaat atttttaagt gtaggatatt caacatgtca
    11041 ttggtgccat gttatggaaa aagaatcttt tgaagatgaa gaagttgctg aaataatgaa
    11101 tagaaacttt gtggcaataa aagtagataa agaggaaaga cctgatgtag atagcgtgta
    11161 tatgacagtt tgccaagcta tgacaggtag tggtggatgg ccaatgacca taataatgac
    11221 accagataaa aaaccttttt ttgcgggaac atatttccca aaatattcaa ggtataatag
    11281 acctggagtt atagatttat tagagaatgt atctgaaaaa tggaatacaa gcagggatat
    11341 attaataaaa tctggagatg aaataataga agctcttaag gatgattttg gtgtaaaaaa
    11401 tactgagggt gatttatcaa aagagatgtt gagttcatca gttagagtat ttaaggctat
    11461 ttatgatgaa aattatggtg gttttggtaa tgctcctaaa tttccaagtc ctcagaatct
    11521 tatgttttta atgaaatatt atagtataga aaaagataaa gatgtgttaa aaatggtgga
    11581 aaaaacttta gatggaatgt atagaggagg cctgtttgat catattggat ttgggttttc
    11641 aagatactct actgataaaa agtggttggc tcctcatttt gaaaaaatgc tatatgataa
    11701 tgctatgctt actatagctt ttttagatgc gtataaaata acaaagaaag agttatataa
    11761 agaaattgct ataaaaacta tagattatgt agttagagag atgaaggata aagaaggtgg
    11821 gttttattca gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt atacatttaa
    11881 tcctcttgag ataattgaag ttttgggtga agaagatgga attttcttta ataattattt
    11941 tgatataact agtagtggaa attttgaagg caaaagtatt cctaatctta taaaaaataa
    12001 agaatatgaa agacataatg aaaagatagc tgaccttagt aaaaaggtat ttgaatatag
    12061 aaaagaaaga acatctttgc ataaagatga caagatactt acatcatgga atgcattgat
    12121 gatagttgct ttaacaaagg catatagtac tttaaaaaat gacatatatt tagagtattc
    12181 aaataagtgt cttaacttta taaataataa tcttgtaaat gagtcaggta gattattagc
    12241 tagatataga gatggaagtt ccgactattt agcatattta gatgattatg cttttttaat
    12301 ctgggcgtat atagagcttt atgagtcaac atttaatatg aaatatttag aaaaggcttt
    12361 aaatttgaat gaaagctgta tcaacctttt ttgggattat gaaaaaagtg gattttatat
    12421 atatggtaaa gatagtgaaa atttaatagc aagaccaaaa gatttgtatg atggagctat
    12481 tccatcagga aattctgttc aattatacaa tcttataaga cttgcgaaaa ttactggaga
    12541 taatagactt gaagaaatgt catataaaca attaaaactg tatgtggata atgtaaagag
    12601 ttctccaact ggctacagct tttatatgct ttcattgatg tttgaacttt attctactaa
    12661 ggaaattata tgtattttta aggaagattc tgatttaatt gcatttaaag aattgataag
    12721 tgaaaatttt ataccaaatg caactttttt agcaaagaaa tacaatgaag aaaacactat
    12781 tataggtttt ttaaataact atatattgaa ggatgataag actagttatt acgtgtgtca
    12841 aagtaatagt tgtagtcaac caattaatga tttacaaaaa ttaaaagata tgattttagg
    12901 agtagaataa tcataatagc gaaaaaggag atgctttaaa ataaaagtct aatcatctcc
    12961 tttttttgta caaaaaacag aaaaagagtt cctaagaaca cctaatctat gtaatgattg
    13021 ttttgttaaa aaataaaaat gacattatag ttatataaaa tattgtacta caaaaaagta
    13081 gtattataga tgcgatagta ttaatcgttt ttatttactc tctttcttct ttttgcaaat
    13141 tcttcaagtg cttcagattt aattttatct atttcttcat attccataaa attagcatct
    13201 tcttcaaagc catcttgttc ttcaccatta aaacgataca ttttttttag taaactccat
    13261 aaggtctcta attcatcttt ggtaaattcg tgaaatacat ctgccataaa gtttattcca
    13321 gtcctactac atgtcaccat aacctttttt cctaagtctg taactttaac attaattgct
    13381 ctcttatcat gaggacttgg aattacatca acatatccat ttttttcaag attagcaact
    13441 agtctattta tattttgttt acttgtaccc atttttcttg caatattatt taatgttgtt
    13501 tcttcctctg gtaaatgaag aattgataaa atagtcatat actgtcttga agtaagtata
    13561 ccaaaatatt tatctccttc tatttgtatt ttgtttgtaa gggtaaaaag agttgcatat
    13621 gtttgattca ttaaaaagag ctcttttagt tcatttgaat aatccatgaa atatatctcc
    13681 tttattttta gatgttgttt tgtcgaaaac agaatatcat gaattaattc taatgtcaat
    13741 atggtgtcaa aatatattaa agtgttactt ttttatgtaa ctattaatta attaaattat
    13801 tgtataattt tatgtaaaaa cttctatgta ccaatgataa cttctatgag gaattaataa
    13861 ttcttaaatt ttttagaaag attttgactt gaaaatactt gtaatataat atataaatta
    13921 atatactgaa taaaatcaat ctggagggat aattatgaag tgtacaaact ttactttcaa
    13981 aggagaggaa ggtttagata tatatacata taaatgggaa gatgaaaata taaaaaaacc
    14041 aaaggcagtt atccaaattg cacatggaat ggctgaaact gcacaaaggt atgagacttt
    14101 tgccaaggta cttactaaaa atggttatat agtatatatc aatgaccata gaggtcatgg
    14161 aaaaactgca aagataatag aaaatgttgg acatttagct gaaaaagaag gatttagatg
    14221 tcttgtagag gacatgtata ctttaacaaa tattataaag aaagaaaatg aagatttgcc
    14281 aatttatcta tttggacaca gcatgggttc atttgcaagt caaagatata taatggatta
    14341 cagtaataat ttatctggac ttatactatg tggttcaaat ggaaagcaag gaatcattct
    14401 aaatcttgct catctgataa tcaatcatga gattaaaaag tatggaagac gttttaaaag
    14461 taataaaata aataatttga tatttggcgg cgaaataata agaagaaatg aaaagactaa
    14521 gtttgactgg ttaagtagag ataaagaaca agttgaaaaa tatataaatg acccattttg
    14581 tggagtagtg tgtagttgtg gcttttttta tgatttagtt caagggttaa aagaaattga
    14641 ggataaagaa aacttaaaaa aagtgccact tgatatacct atttacataa tatctggaga
    14701 taaagacccc ataggaaaaa atggtaacgg agtcttaaga ctaagggata gatatataaa
    14761 attaggagta aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg aattgttgaa
    14821 tgaaataaat agagaagaag tttttgagga tataatttgt tggttaaata ataaaataga
    14881 aatattgtaa taaaaaaaat ataatgatat atttacttta aatttattta tatataatat
    14941 ttgaaaagtg gattatgtat atgcaaagta gaaaagagag agcctagaag tgatttttcg
    15001 ctacaatgcc atttattgaa taggtgggtg ttattataaa taaattatta acactcagta
    15061 ttattatcat tgtatatgga atttatttta tttaaaattc cttgaaagtt taaatatagt
    15121 aagttgtagt aaagagaaaa tctaaatatt taaatgctat ttaatgtgaa tctattaatt
    15181 aggttatact taaacttaat taatagattt tatatttcaa gctaatgaga tagggtttta
    15241 gattaaaata taaccaatgg ttaaattaca gacattttgt taactttccc tcgatttact
    15301 agtaaatatt tttttaatat acttaaatta caacagatgt actagggagg tatatatgca
    15361 aattaaaata ggtaaagtca tacagcgtct tagaaaagaa cgaaatttaa ctcaagaaca
    15421 attagcaaag tttataggtg tgtcaacacc agcagtatct aagtgggaga gtggaaattc
    15481 ttacccagat atagaattat tgcctttatt agcagacttt tttaatgtgt ctattgataa
    15541 gcttttaaat tacaagattg atttaagtga agaagaagta atgaaaattt ataaagaatt
    15601 agaatcaggc tttgctagaa ttgaaattga tttatcaaca gaagaaccaa aagaagaatt
    15661 tagacaagac ttagaatctg taaaaaagct ttccaatatg tatatagaga agtatcctaa
    15721 aagctattta ttgaagctaa ggatatgttc gctgtatcag atgtattcat ataaatttgg
    15781 taagagtgaa cttaatgaca gagtaaaaga aacaacgaat attcttgaag atatagtgag
    15841 aaatacagat gatatccaaa taaaagaaac agctcttatt atattatcaa acgcatattg
    15901 tatgttggag gattatgaaa aggcagagtt gtatttaaat atgatacata agtcaatagg
    15961 ggatacaagt gttaatttag ctatgatata tttaaagcaa aatagacttg aagaagcaga
    16021 aatattgctt caaaataaat tgtttagtaa tgtatttaat ataagcatgg attgtaaggg
    16081 aataattaat gtatacaaaa atcaatacaa ggaattaaag aaaaaattgg agaatagaaa
    16141 ttttaataaa aatgcaattg agagagaaat ggagtatata aaaaataagt tgctggggta
    16201 tgcaaatctt tctttagaaa taaaaaaaat gctcagtgaa gataaaggtg cattttttag
    16261 tatgtatatg gattatatgg aattatctct aattttttta ttttttaaca tgaaagaaga
    16321 agcaaaaaaa gccttatata gtctaaaaga aatattagaa aaatatccta tacatgaaaa
    16381 tttggatgta agtcaaatga gattttttga taaggtagag tctaaaaatt tatatacttt
    16441 taatatatat acaaacttgt taattgtact taatgatgat agttataatg agcttagaga
    16501 agaaccaatt tttaaggatg taattgaaaa gattttagat atggaaaaaa tgttgaaaaa
    16561 taaagaataa gtaaaaattt aatataggac ttaaagatta atcaaattaa aagaaagtgt
    16621 ttcaaaataa aaatttgaat cacctttttt gtctgtctta aacagttatt tagaaaaata
    16681 gaggtttcaa aatagaaatc tgaattatct gtattattac acaaaaatag aaacctaaat
    16741 ttataaaaat agaataaagt ttgttatata aaatgtaatt ttgtctataa aatctcacct
    16801 gttttctaac tttaagcgtg aaaaagagaa tgtatcatga attttttagt tcatgatgca
    16861 tccttctttg tatgaaatcc aatatatctt tattatttaa acaatggaaa agagtcttat
    16921 ttctattttg agacatctct tttatttaat tatgaagttt tatacagttc aataaatagg
    16981 aattatattt agaaactcat gacattattt cttagatagt gattaaattt atcatattcc
    17041 attttagata acttttctgg gtaagacatt acaatcaggt tatgcccatt aaatttttca
    17101 tggtatggtg gatgaatgtg ttcataatta tttgtcttat attttactct ttcatagaac
    17161 gccttgcgat aaattgaaat ctcatcaatt ggaaggtcaa tttctaaaat aatagttttt
    17221 cctttcttat ttaaaagctc taacgctctt tgtccatatc tattattgcg catttctgga
    17281 aaaatgcaga aatgttctac atagataaag tcatctgttt cccaacataa aattatgccg
    17341 acaaactggt ttttattata aattaaattg aattgatatt ctttattttt caaaatttct
    17401 tcttgcaaag aagattttct ttgttcatga aatgggaaac taattttata taattccata
    17461 gccttgtaat ataatttatt ttcactggtt cttaaccgtt caaatttcac tttactcctc
    17521 ctaatcacga aaattccaat ttatttatga gttcatttta ccatagtcag tatttgcata
    17581 caatgtgttt taagttgagt gtcatttctt tcttttattt atgtttatat ctcatagact
    17641 aagagataat tacttaagac ataactaaaa actattatat taagctttga taatctaaaa
    17701 acagtatatt tatggacttt tctaggtcta aaagtaatct taataatttt agaagttaca
    17761 aaaaagttac ataaatttgg aaagctataa tatacatata taatataagg ttaaaactgt
    17821 aatgaataat attaatttaa aatttgaaca tggaaggaga ttattatgga gaaaagagaa
    17881 aagaggataa taatatcatc tttattaagt gtatcaattt taatgggttt ggtaagtata
    17941 tattctatat taaataaaga agacataatt ttaactgtta agggtcaaga gcaaaaagta
    18001 tcttccttta agaaaacagt tgaggaactt ttggatgaac agggtgtaaa gtataattct
    18061 gaagataaga ttaatccaag tttagataca gaactaaaag atgatatgaa aataaaagtt
    18121 gttaaggtaa ctaaaagtaa aaaagaagag attgaaaaaa ttccatttga tacaaagcat
    18181 gtaaatgata gtaatttgtt aaaaggaaaa tctaaagttt atcaagaagg tcaagaagga
    18241 gaaaaaaaac tagtctataa tttaacttac catgatggaa agttagtcaa aaaagtctta
    18301 tcaaaagaag taatatctaa ggagccaact acaaaaatta taaaatatgg aactaaagaa
    18361 aaagtactaa tagcatcaag aggagcaaat ataagaggag gcaaacatat gaaagtggtt
    18421 gccactgcat atgcaggaga tacaataaca tctactggta caactccaag atggggtgtt
    18481 attgcagttg acccacgtgt aataccatat ggaacaaaag tatatatacc taaactgggt
    18541 atgacttttg tagcagaaga ttgtggaggt gcaattaaag gtaatagaat agacattttt
    18601 atgaacagtg aaggaaaagc ttcaaattgg ggaaggaaaa gtatagatat atatctacat
    18661 taaattagtg aatagaataa ctttacttga aggacaaaaa agtataaaat atgaattaac
    18721 taaactgtta gattattctt aaaacataaa aatatatcta aggggggata agatgaaaat
    18781 agtctgctta ggagacagtt taacgtatgg atttggggta tcacgaagta attcatggac
    18841 aaacattgta aataaagaga ctcgattaga aatagtaaat aagggtataa atggtgatac
    18901 aacaagtggt atgttagcta ggtttaatga agatgttgta aaaaactctc ctgatatagt
    18961 ttttataatg ggtggcacca atgattttat tgctggtgcg ggaaataaaa ttattaattc
    19021 taatataatg gctatggtgc atcagtcata ttcaaaaaat attataccta taataggtat
    19081 accattaaaa ccagatattc caaatgttag ggaagattgg agttgtttta ctgattttaa
    19141 tatagtatct caaaacttag aatcatatag ttattggata aaaaaatttt gtatgacatt
    19201 taatactaat ttcgtagatt tttattcaga atataataaa aatatggaac tagaaggata
    19261 taaaaaatta tattttgatg ggttacatcc aaccaaagag ggtcatagaa taatggcaga
    19321 catttttatt aattcaatta ataagtatat agaagagtta tagattacat taaaatgctt
    19381 gtttatgcgt cagttgcaat aaatacatac agtagagagg ggtttattat ggattatact
    19441 aaaacttatg aagagtggat aaagggttca tattttgatg aagatacaaa gctagagttg
    19501 gagaatataa aaaataatga aaaagaaata gaggatagat tctataagga tttagaattt
    19561 gggactgcgg gtcttagagg aataattgaa gcaggaacga atagaataaa taagtacaca
    19621 gttagaagag ctacttttgg attggcaaac tatatattag aaaacacaac aaaagaagaa
    19681 acaagtagag gagtagtaat agctcatgat aatagacaca agtctagaca attctgtata
    19741 gaatctgcaa atactttggc agcatgtggt ataaaagcat atatatttga tagtttgaga
    19801 actactccag aattatcttt tgctgttaga agcttaaatg caatcgctgg tatagtcata
    19861 acagcaagcc ataatcctcc agaatataat ggatataagg tatactggga agatggagca
    19921 caagttatgc cagagattgc aaatgcaata acagagaaag taaatagtat acatgattat
    19981 agtacaatac caaccttaac ggaagctaat aaaaatttgg tagttttgtt ggacgaatca
    20041 caagatacta aatttataga agcagtaaaa agccaaatta taagaaaaga tttagttaaa
    20101 aatgtaggta aaagctttaa gatagtttat acacctcttt gtggaacagg gaatgttcca
    20161 ataagaagag cattgaaaga agttggtttt gaaaatataa ttgtagttcc agaagaagaa
    20221 aatccagacc caaattttgc agggcttgat tatccaaatc cagaagagaa aaaagcttta
    20281 aatagaggta tattactcgc aaaagaaaaa ggagcagacc ttgtaatagc aactgaccca
    20341 gattgtgata gagtaggtgt agcagtaaaa actactacag gtgagtatgc tttacttaca
    20401 ggaaatcaaa ttggtggtat gctgacacat tatataatag aaggattaaa agaaaataat
    20461 aaattaaaag aaaatcctac aataataaaa accatagtta catcagaatt tggagcagat
    20521 atagctaaag caaataatgt tgatgtatta aatgttctta caggttttaa atttattggt
    20581 gaaaaaataa aattatttga acaaaataaa aatagaagtt atgtatttgg atatgaagaa
    20641 agttatggtt acttagttgg gactcatgct agagataaag atggtgtagt ttcttcattg
    20701 ttaatatctg aaatggcagc attttactat tcaaaaggta tgagtttata tgatggttta
    20761 atagaacttt ataaaaagta tggatttttc aaagaacaga ctatatcttt gactttaaaa
    20821 ggtatagaag gtgtagaaaa gataaaagaa ataatatctt attttagaga aaatcaaatt
    20881 gattatataa atagtattaa agtagttgat aaaaaagatt ataaaaatgg tatagataat
    20941 cttcctaaat ctgatgtttt aaaatacttt ttggaagatg aatcttgggt agctataaga
    21001 ccatctggaa cagaaccaaa acttaaattt tacatagctg taaaaggagc tagtgatatt
    21061 gaagcagata aaaagattca aggtttgaaa aagtatatag atgacatggt cgaaaaatta
    21121 aaataaaata ttattatttt ataagataat tttgtaataa tatttattta gctgaaaatt
    21181 ataatttact ttcattgagt agaatatgga aatgttagta gaattgttat tttcagcttt
    21241 tatttgtgtt atttttactt aaataattat attatagaat gaatctgagt ataattaata
    21301 ttgtataatg aaattaattt gatagaaaag gggatattta tgtttggtat agataagaaa
    21361 ttgattgaat atataattgc aaaatccaat tacagtgaat taagttcaaa gtcagcagaa
    21421 gtatctttct tcttaatgtt gtccatattt ccttttttaa tatttacaat aagttctata
    21481 gcttatatac cgattcttca tttaaataag tatatagctt tgtttagaaa tatgatgcca
    21541 gaaggtgctt ttgctgtgtt atcttcaatc atagtttctg caatagacaa tagaaacctg
    21601 aaatttttag cagtcagttt tgtacttact atgtggacat tttcaagagc agtaaaagct
    21661 cttattaaag gaatgaatag agcctataaa gttaaagaaa ctaggtcttt ttttaaaatt
    21721 ttatctatat catttttatt tacaataatg cttttggtcc ttattttttt atctatgata
    21781 tttttagttt acggggaaaa aataggttat tttattttta atcttgtagg attggatgag
    21841 atatttataa aaatatggga tattctaaga tacactgtag gtataataac aataatagtg
    21901 atatttacac ttttatataa atacactcca aataaaaagt taactattaa agaatctgcg
    21961 ccaggtgcta tttttgctac ttttgcttgg tttttagtat catttttata ttcttattat
    22021 acaaattact atgctaacta tgaagtaatc tatggaagta tagctgaaat aattgtactt
    22081 atgacctgga tgtattttag tagttggtct atcgttattg gctatgaggt aaattcaaga
    22141 ttgtatttta gaaagataag acatgaaatg ttaaagtaaa gtatttaaaa taaaagagag
    22201 tatttagtat tataaatgta attgaattaa taaagaaata ttgacattta agttaaaaag
    22261 tctagaattt ttctgggctt tttttatttt tagtataact tgacaaattt tatttaatgg
    22321 gatagtttta atttataatc atctttaaaa agatgactat tgataagtaa gagataaaat
    22381 tggtaagtat caaaaaatat actaaataaa aattatagtc ctaatgagtt aatttctgta
    22441 ttttcaaata atgcagtatt tagtcatgtg tttttatatg tgcctgtatt tctaattacc
    22501 aatatattag aataagctac tgtaattaga tttagaaaaa agcgacataa ataattataa
    22561 acaaataata aattacataa aattaaattt attcacaagt atatcttata aaatatgtta
    22621 taattaatag aaaattgtca tataattaat taagtagtat tattataaat aacattgaaa
    22681 ggtagtggtt agaatatgga tacccataat aaatatgtaa attttataaa aaatatacct
    22741 gtaccttttt tatattgtag aattgtaaag agacaagaag atatagaata tcgagtagaa
    22801 tatataagta agggtatggg caaagtatta caattagaag aaggtatttg tgataaaaat
    22861 atactagatg tattgcctgt atttaagtct aaaaaatatt tcaaagagtt gttctcaaat
    22921 gaagtggatt gtataaaaag gtacattccg acacttaaaa actggataaa tataaaaaaa
    22981 cagataatag gggattcata cataattttg tattttggta aaattgtatt tgattataga
    23041 cagataatcg attcctttga taaaaaggaa aaagtagctt atattaaaga cgaagaaggc
    23101 atatatatag attgcagtga aaatttaata ccaatattaa ataacaacat taaaacaacg
    23161 aaagatatct ttggtaaaaa tgatatagaa gtatggggag agaatacagg aaaattattt
    23221 agagatgatt atagagaagg agtatctagt aaaaaaagat ttttacaaaa tctttttgaa
    23281 tatgaagaaa cattttttat ggttgaaaaa tattttttgt atgatgaaga cgaactttta
    23341 ggtactatag ggatagtaga caatataata tatagtggtt acagtaatag aaattataac
    23401 tcaaaagatt taatgaagat gatagaacac tcaattccag agaatatgtt ttataaagat
    23461 gtgtatggaa attatattgg atttaattca ggttttttga atttagcttg tatgaataaa
    23521 gaagaattat tagggaagaa tagttataaa atatcagaag aagaagcttt aatagataag
    23581 atatttgaaa gtgataaggg tgtagttgaa aataaaaaag tagttacatt tgagttaaat
    23641 ataagtatga atgatgaaaa caagtgcatt gagattacaa aaaggccgtt ttttgatagc
    23701 tatggaagtg ttattggaat aattggaaca gcaagggata taagtagaag aaaaagatta
    23761 gaagaggaaa tggataaaac taggatggaa ttttttgcaa atttatccca tgaacttaga
    23821 acgcctataa acttaatatc atcttcatta caagtgatag aaaaaaaaga agcagattta
    23881 atagaatcaa atgatacttt aaaaagaaat ttaggtataa taaaacaaaa tggaaataga
    23941 atactgaggt tagtaaataa tgttatagat tttacaaaaa tgcaatcagg atatttagat
    24001 tttaaaccag aagaatctga tataattgca tttatagagg agatatgtat gtctgtagct
    24061 gattttgcaa gccaaaataa tatacaactt acatttgata cagaaataga agaattttca
    24121 atgttatttg attcagaaaa attggaaaga ataatattaa atttactttc taatggaatt
    24181 aagtacaata agaaagatgg aaaaataaat atatttttat atgtgaaaga taatgtattt
    24241 aatatgaaaa tatctgatag tggtataggt ataccaaaag aaaaaataga taaaatattt
    24301 aataggtttg aacagattga caatgaactt tcttacagag ttaaaggaag tggtattgga
    24361 ctttcattag tgaaatcttt ggttgagttg catgagggaa gtatatcttt aaaaagtcaa
    24421 cttggtattg gaagtgaatt tatagtttca ttgcctgtta gaagtaaaaa taatattgaa
    24481 aaatataatc ataaaagaga aatcagcaat gaactaagta aaaaattaga aatagagttt
    24541 tcggatttat aagtttaaat ataaaaaata aaattttaaa aaagtattga caattgattt
    24601 agaagggttt aaaataaatt acaacataat aaataaaaag gcgatgaata ggaagagtaa
    24661 atctaattcg attaccagag agaagagtcc gttggctgga agacttttta aattagaggg
    24721 attgaaaact acctaggagc tactttctga agtcatctgc attagtagat tttagtaaga
    24781 atagtcgtta gctgcgttaa agtgtaaagt gagttttcat gacatatgaa aactaaatta
    24841 ggtggaaccg cgggaataat actctcgtcc ttatgttata aggatgagag ttttttgtta
    24901 tttaaaaata taaacagtaa tcataataat tatagaaaat ataaaagcaa tgaataggaa
    24961 gagtaaattt aattcgacta tcagagagga aagttcacag gctgaaagac tttctaagtt
    25021 tgagaaattg aaaactacct aggagctgtg gtctgaagtt actttaaaaa gttttagtaa
    25081 gactaaccgt taactgcgtt aaagtgtaaa gtggatttta tcaataaatg ataaaatcaa
    25141 attgggtgga accgcgagta tagactcgtc cctttatata gggatgagtc tatttttatt
    25201 gcaataaaag attgaaaata aaaaaaaatc ggaaaattat aaatttagga ggaaaataaa
    25261 atggagaata aagtgaaaat tggagtacta ggatatggag ttgtaggaag tggacttata
    25321 gatataatag ataataataa agaaaaaaga aatatagaaa tagttggaat tttagttaat
    25381 aatttggaaa aacataagga taaaaaatat tctaatataa taactaataa tatagatgat
    25441 atatttaata aagatataga tatacttgtt gaagtcatgg gtggtttaga accatcatta
    25501 tcttatataa aaaaagcatt aaacaataaa attcatgttg ttacagcaaa taaagatttg
    25561 ttagctgaat gtggagatga gcttgcaaaa ttagcaagtg aaaataaagt aagtattaaa
    25621 tttgaagctt cagttgcagg aggtatacca gtattaaaac caataataga atcactagaa
    25681 ggaaataata tagatagtat aaatgcaatt ttaaatggaa ctactaattt tatattatct
    25741 aaaatgtatg atgagaatct atcttatgat atggcattaa gacaagcaca agaattaggt
    25801 tttgctgaag caaatccaga gtcagatgta ttagggtatg atgctgctag aaaactatca
    25861 atattatcaa ctctagctta tgacaataga gtatattgga aagatgtgta tttagaagga
    25921 attacagata tagatgaaaa agatattgaa tatgcaaaaa aattaaattg taaaataaaa
    25981 ttgattggtc aaagtaagta tgaaaatgat aaagtaagtg cttttgttag acctgtttta
    26041 gtagaaaaag ataatatcct tgctagaata gacaatgagt tcaatgctgt catagtgaat
    26101 ggagattcag taggagaagt ttcttttgta ggtaaaggag ctggaagttt agcaacagga
    26161 agtgcggttt attcagatgt tatagatata atagataata gagtttcaag tatagattct
    26221 tttactaaag ataaaataca agttaataaa atagttagag aaaagtgtgg agcacttctt
    26281 agatttaaaa aatgtaataa agatgaaatt ctaaatatag ttgagaattg tttagtaaaa
    26341 ttcgatattt taaatgatga tgatgaactt gctattatgg tttatgctga ttctgaatat
    26401 gaaataaaca acagtttatg tctaataaaa gataaaggat actgcgaaaa aatgaataaa
    26461 atgttgaaaa taagttaaaa aagaaaaaat ataagtttat atgtaacata ttttgacgat
    26521 tttaatacta tatagtatga gatgaaacaa aacatctcat aaaaaaatta aaattaaagg
    26581 gagagatatg ttatatggaa aataaaaaat gttattcaga agattggtat gaaagaggag
    26641 aatctacagc taaatggttc caaaatgata gagaagaata tgaaagagaa gcatatgatg
    26701 aagatagaga aagaagaggt tcaaactgtg gatgttcaga ttcaggagaa aatagaccta
    26761 gaaactgtga aagatttaga agagaagctg agataagaga aagagaagca agagaagcat
    26821 tctgtgaatc ttcagagaaa aagaaagagg cattagcata tgaatgtgaa gctagaaaat
    26881 tatgggaaga agcagaaaaa tactgggatg aatattcaaa atacaactat aaaggaatcg
    26941 aatatttagc agaagctgct agattatttg atgaaggtat ggaatgtgaa gctagaagaa
    27001 atggaaataa tggaggaaac aataataatt gttgccataa atgccataaa tgtaattgta
    27061 actgctgtag aaaataaaaa aattaattga caaatcaata gttaaaatat atcatattat
    27121 gtataataaa gagaaaattc agaaaaaaat aatgattaga agaaatataa gtatatttat
    27181 tagacaaata gtgctcagtt agtgaggtga gtacaatatg attaaactcc catcctctag
    27241 cttttgatag agggtgggag tttttattta tttatatatc ttaaatgagg ataatattgt
    27301 aaaataaatg agtatgagat tgtaaaatta ggaggtaaaa aatgaaaagt attaaaacat
    27361 caagtatgaa agaaaaggta gatgaaaaga tattaagaga gcaagtaagt tctataatag
    27421 aagatataag aaataataag gatgttgcac ttaaaaaata taatgagaag tttgatagaa
    27481 atacaagaga tgaatttaga attactaagg aagaaataaa ggaagcttat aagcatgtag
    27541 atgacgaatt tataaataac ttaaagattg ctgccaaaaa tataagagaa tttgctaaag
    27601 ctcaaaaact aagttttgaa aacccttttg aaaaagaaat ttatccaggt gtaatcttag
    27661 gtcaaactaa tattccaata gagtcatgtc ttgcatatgt tccaggaggt cagtatccat
    27721 tgttttcaac agctcttatg cttataatac ctgcaaaagt agcaggagta aaaagaatag
    27781 tggcatgttc accaactatg aaaaatactg aaaagataaa tcctaagaca ttggtagcta
    27841 tggatatagc aggtgctgat gaaatatatg caactggagg agttcaagct atagcagcat
    27901 ttacatatgg aacagaaaag attaatccag ttgacataat tgtaggacca ggaaataagt
    27961 ttgtaactga agctaaaaga caatgttatg gacaagtagg gattgatttt gtcgctgggc
    28021 caagtgaagt tcttataata gcggatgaaa cttcaaatcc agtttatatt gcagctgatt
    28081 tattagcaca atgtgagcat gatttaaatg caagaggaat attattgaca aatagcttgg
    28141 aagtagcaca aaaagttgaa aaaaatatag aaagtatgct aaaagatttg ccaacaaagg
    28201 acatagcata tagttcatgg gaaaataatg gagagataat attggttgat gatatggaag
    28261 aagccattaa aataagtaat ttttatgcac cagaacattt agaagttgca gtaaatgagt
    28321 ataatgatat ttgtgataga ttaactaact atggttcttt atttattgga aatctttctg
    28381 ctgaggtatt tggcgactat gtttcaggta ccaatcatac attaccaaca cttaaagcat
    28441 caagatatac tggtggtgtt tgggttggaa cttttatcaa aacttgtaca aagcagatat
    28501 ttaatgaaaa agctatacaa tcattagcac ctgtagctga aaagctggca aaagaagagg
    28561 gattatatgc acatgcaaag gctgctgagg ttaggtttaa aaagtagtaa aaaattttat
    28621 tttggcagtc gagatttagt gttagactga gataaggaaa acgctgagaa cccgaataaa
    28681 tactatattt tgtgggcctc ggcgtttctt ttatatctgt atataatagg ataatactac
    28741 ctgtgtgttt aaatactaac ttaatatttc ttttctaaat aatgtttatg aaatagttaa
    28801 tatttttagt taatagttga aaaaaacttt tgctaactgt gcaaattcat ctgcatttgt
    28861 caatatagca gggtgtcctc cctgactaaa aatgtgaata cttgcttttg aaataaaagt
    28921 tgccatttgt ttgtactctt cttcaagatt atgacgacac atttcgtctt ccttacttcc
    28981 tgtaaataaa attggcattt ctaatttaca caattcttta tgaaacaaag ggcgtttctc
    29041 gtttgcacat tgcaatagag cttttgtatc aagattaaca acattctccc aatcttttcc
    29101 atgacaccat tcatagaatt ttcgtgcttg aatatcttgc tttgattttt cccttccagt
    29161 gattagatta tctatgaagt tatcattaag agtacgtcca tcaaagctat ctgctataat
    29221 tgcttcaact aaatcgggac gttctaatcc agtattaaca gctgcccatg ctcctccact
    29281 tgttccaatt aagccaactt taccacaatt caaatgttca atcagtgcta ttgtctgtaa
    29341 ggcttcatca tgccatatgt caggagaaaa ttgagaaact ctatccgact gtccattacc
    29401 taaaaaatca attaagatac atctaaaatt ttctgcatat aatggcacaa gtgctttaaa
    29461 catattagaa gaaccagtat taccatgcag gaaaattatc ggtttacctt gtccatattc
    29521 tttataaaaa caagaattac cttgatactg aaaatatgac atatattttc ctccttttta
    29581 tttgataagt taattataac aatataggta tgaatgaaaa acctcatgtt ctttcttaga
    29641 ataaaaatgt gccacgcata aaacgtgaca catttctaat tataaatttt gctgtttaag
    29701 tattctttgt atgcttttct ctgtcaaaaa gtatttctct gataactgct tgatactcat
    29761 tccattttgg aaatttatac agatttgaga attacgagaa gctaagactt gtttggtgtt
    29821 tgtgttttca ccccaatgct ttttgtgctc ttgctttttg ggaatataaa tcactttacc
    29881 atctatatat tcttgcactt gctcaatcag ttctttaggc agtaagtcaa tcgaattttg
    29941 atagctcatt ttgcacctcc taaataatta ttctttagga ttagcaaagg ctactgccac
    30001 tcacaattaa aaataaaatt ttttattatg tacaatagcc tttgctatgc acaagttcca
    30061 attcccataa actaaatact cctttctggt ttgaaaaatt catctgaaca ttgatttcgc
    30121 ttttaagata gagttcaatg acaaatttac atttttatta tatatattca gatagataat
    30181 tgcaactgtc tttgtatgag gatattttat tatagtcaat actataatat gtatttatat
    30241 catcaaacta atatgaacag ttgaaaggaa tatgaacaat aaaacttagt caggaagaat
    30301 aattatgaag cagataatag agaaatttga ttttaaagtt ttattgaaaa tttattgaca
    30361 aatttaactt gtaaatatta tactaaactt aataaataaa gtttataaag taaggtgttg
    30421 tctatgaaaa atatagttgg aaaaccgcaa acgataaaaa aagtaaatga agatttaata
    30481 aaaaacatta taaaaaatga aggtcctata acaaaaccag aaatttcaag cattactgaa
    30541 ttgagtttag ctacagttaa taagattgta gagcaattag ctttgaaaaa tgaagttaaa
    30601 gtaagtggac taagtgaatc tactggagga agaagagctc aattatttga aataaatgca
    30661 aacttagaac acataatagc tttatattat tatagaaatt gttgtatagg ggtagttgcc
    30721 aatttattag gtgaaataat ttatcaagaa gaatttaata taagaatgga tacgtatgaa
    30781 aacgttaacc aagatacttt ttttgttata gatactctaa taaaaaggga aaaaggaaac
    30841 aatataaggg ctattggaat tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat
    30901 ataccaacta taaaaagttg ggaaggtata aatttagaaa atttaataaa gagtaaatac
    30961 aatactaaag tgtttataga aaatgataca aatattacta ctattgggat ttatcaaaaa
    31021 cagtataaag acaaatataa aaatatggct cttatgtatt tagaagatgg tattggttca
    31081 ggtatagtta taaatggtga attgtattta ggttctacaa attttgctgg agaattaagt
    31141 tatctaaata tagaatttgg taagtataac tctaacaact taaatcttga agattatatt
    31201 atgagtttaa gaaaaagata tttagaaagt aaagataata aatttaagaa agaaatttta
    31261 tctataatat caaactttat aaggactctt gtatgtgtcc taaatccaca agctatgatt
    31321 atacagtgta gtatattaac caagagtgac atggagttta tagaaaaaga tgtaagcaat
    31381 aatgttggag aagaaaattt tcctaaatta ataaaagtag agtcattgag agaaggaagt
    31441 ataaatggag taattagtat gtgtttaaaa gaaactgact atcaatattc cttatctaat
    31501 aaaaaaggag gatattaatg tgaaatattt ggtttcaata gatggagggg gaacaaaaac
    31561 taaattttgt gtaagtgact tagatggaaa catacttaaa gaacatacca ctggttcaac
    31621 taattataag tcagttggaa ttaaaaaaac atatgaaaat attaataatg gatttaaaaa
    31681 aatattaaaa gatttatata tcgattatga cgatattgag tacactgtat ttggtatatc
    31741 tggttgtgat tctcctaatg attataagat tataatggat gaaatattaa aaattgggat
    31801 taacaaagaa aaaatatatc tggcaaatga tgcagtttta gctttttatg cacaagctga
    31861 ttcccctggt ttagtaattg tggctgggac aggttcaatt atactgggaa ttaaagaaga
    31921 tggagaaata tatagagttg gtggatgggg gtataatttt agtgatttgg gttctggata
    31981 tgatataggg agaaaattat taaagaaagt attgctttat tgtgatgaat gtcatgagta
    32041 tagtgattta tttagttgtg tcttggattt ctttggagca aatagttttg aacaacttgc
    32101 atatatgatt actgatataa atgataatgt tgaaatagca aacttagctt cattagtgat
    32161 tgattgtgcc gaacaaggtg ataaattggc tattgaaata ttaagagaat catcaactga
    32221 actatctaaa ttagctcaag taatattgag aaaaatatct aatccaatga aaatggataa
    32281 aagcgaaata aatatagttt tgtctggtgg tacattaaat aaaactgtat atgctcaaat
    32341 gttaatgaat aatttaaaaa cagaaaatac agataaaaat ttaaaattta tatctcaaga
    32401 aaatcagcca gtatatgggg ggataagact tgctatggca ttagctaata ggagggccaa
    32461 gcatggatag aaaaaaggta atagtacaaa tttttactgg aggttttaag gctaaagaaa
    32521 taaagtttaa tgatataaaa tgtaaactac tagaattatt ggactgtatt gatatagaaa
    32581 aggttatcat gggatggagt gtagatgaag aattatatgt agaaactaaa aaactattaa
    32641 ataaatataa tgttgaacta tatctttggt tgccatgttt ttctgaaata ggattacttg
    32701 aaaaaagtaa tttactaatt gattatatgg ggaaggaagt caagggctat gctttacaag
    32761 aggatgaaaa ttttgagttt tattgcccaa ataataagga aaatataaaa aatataaaga
    32821 aaatatatag tacttatttt agtgatatta aatttgatgg aattttttta gataaaataa
    32881 ggtatggttc tttttcgaat aaactaaatg gtgtttttaa ttgtttttgt aaagattgtt
    32941 taaatatata tgaaaaaaat aatattgata aagagtcgtt aatgagagaa atgaataatg
    33001 tgtctaaggg agttatagat aatgaagata ctccatttgg tataacagaa tacattaatg
    33061 gaaaatacaa atttaaaaat aaaatatggg aagatttttt tgaaacaaaa aatcaaaata
    33121 tatacaaggc attattagag ataagtaatt attttaaaag taagggattg aaaataggta
    33181 tagatacttt ttctcctttt atttcttact ttacaggtca agatttagaa aaattacaag
    33241 aagtagcaga ttttataaaa ccaatgatgt atagaattac aaaagctcca gctggtttac
    33301 catttgaatt tgaaaggttt attgatgaat ccatacataa gaataaagag attggaaaaa
    33361 gaaatttctt agatatttta ggaattaagg agtatgaagg aaatgtatac cctatagaat
    33421 ttgtactaga ggaaatcgat tttatgacaa gaaatcttaa taaagatata tatgtaggag
    33481 ttgaaataaa taaaaaggaa attatagctc ctgttacacc taagtacata agtgaaaata
    33541 tttcaaatat aaataagaca acagtaaaag gatatgtttt atcttgggat ttgttatcag
    33601 caccaaaaga aaatattgat gaaattataa aatggtttcg gaattagtga ggtgatatag
    33661 tgagtaagat attggaaatg caacaaatat ataaaagttt tggaaaggta gatgttttaa
    33721 aagggataaa ttttgagtta gaaaagggag aagtaagagc tcttttggga gctaatggag
    33781 caggaaaatc tacacttatt aaaattttag gaggagttca ttcccaaact aaaggtaaag
    33841 taattttgac tggtaatgaa ataatgttta aaaatccaca tcattctaaa gaaaatggta
    33901 ttagtataat ttatcaagaa ttaagcttga taccaacgct tacagtactt gaaaatattt
    33961 ttcttggtag agaaatttta aaaggaagtg tgtttttaaa caagaaagac atgcttgagg
    34021 aatatatgaa aatttgtgag gattttgatt ttaatataga cccacatata gtagtttcta
    34081 atttaagtgt agcaaaccaa caaatggttg agataatgaa agctgtttca tgtaattctg
    34141 aaattatcat aatggatgaa cctacaacat cacttactaa taatgaaaaa gatgggctat
    34201 ttaaaataat aagtagacta aagtctaaag gtaaaacaat agtctatatt tctcacatat
    34261 tagaggaagt ttttaaagta tgtgatagta caagtataat gaaaaatgga attatgattg
    34321 gaacttatga tgtagaggat ttaactaaat taaagataac tcaacttatg acaggaaatg
    34381 ataatcacaa acttattgag aagaaggatt atacatttgc aaaatatgaa gagccaccag
    34441 tactagaagt aaagaatata agctggtcta acaaaataaa aaatgtttca tttaaagttt
    34501 atagaggcga agttgtaggt cttgcaggtc ttgtaggttc aagaagaact gaaattataa
    34561 atttgatatt tggtatagat aataaagata gtggagacat ttttatcaat ggtaaaaaag
    34621 tagacataac ttctcctaag gttgctataa aaaacaaaat aggattgatt cctgaagata
    34681 gaaaaaatct aggattaatt ttagaacaag ctatatacac aaactcatca tcagtacaaa
    34741 ttgataaatt caagaaattc ttgtttatag atagaaataa agaaattgag ttttctaaaa
    34801 aaggtgttaa ggaattagga ataaaagtta gtgatgtaaa gcaagaagta aaagagctta
    34861 gtggtggaaa tcaacaaaaa gtagtagttt ctaagtggtt agagaaagat ttagatttaa
    34921 taatatatga tgagccaaca aagggaatag atattgctgc aaaagaagat attttcaata
    34981 caatcaaaga attttcaagt aaaggaatag gagtaatatt tatatcttct gatttagaag
    35041 aagtaataag agtaagtgat agaattttag taactagaga aggttctatt gtagatgatt
    35101 taaaaaatga gaatatacag gttcaagata taatgaacag tatatttaat gtataaaaaa
    35161 tgaaaatgga ggactgagat gaagaaagct tttgagaagt taaatttaag agattatgga
    35221 gtattattag gttttttagc tttatgtata gctataagta ttgcaactcc atcattttta
    35281 ggtaaacaaa atatacttaa tcttttacga caatcttcta taattggaat aatttcagca
    35341 ggtatgactt ttgttataat atctggaaac tttgatatat cagttggagc agtagcagct
    35401 cttgcaggtg ctataacaat gaaatttgcg actggtggaa ctaatttgtt tgcatccatg
    35461 ttcttaggtg tagcgatttg tgcagtaata ggattaatca atggtatagt agttgcaaag
    35521 ataaatgtac catcattaat agccactatg gctatggtca ctattgtaag aggattgctg
    35581 ttgatgctta caggaggata tccaattact gaaaatatac caatacttga ttacattgga
    35641 aatggatatt tgtttaatat accaatacca gtagtaattt tctttatagt ggtgttagtt
    35701 tcgtttattg tacttaataa aactaagttt ggtagatatg tttattctgt aggagggaat
    35761 caagaagcaa gtaaacttaa tggtataaat gtagattctc acaaagtgaa agtgtttatt
    35821 attaatgctg tacttgcagg aatagctgga atcgtactaa caggaagact tgggactgca
    35881 actgcgattg caggtgaagg atatgatatg gatgcaattg catctgtagt aataggaggt
    35941 acatcagtgg caggaggttc tggttctgta cttaaaactg ttataggagt attacttatg
    36001 agtgttataa acaacagttt taatcttctt ggaatagatg tatatttcca gtacatattt
    36061 aaaggattaa taattttagc agcagttgga tttgattctt atagcaagaa aaaacttgct
    36121 tcaagataaa aaataagggg gattttttta tgaaaaaatt aatgaaacat ttagcattgt
    36181 tgctatcact tgttatgata tttggtttag ttggttgttc aaatggtggt gacaaagaaa
    36241 aaaatggaga aaagaaaata gcagtattac taccgggttc aacaggttac tttgtagcaa
    36301 ctaaacaagg tatagatgca aaatctaaag aattaggtgt aagcgtggaa tatgcagatg
    36361 ctcaatggga tgctagtaaa caattatcac aagctgaaga ttttatggct aaaggggtag
    36421 acatgattgc attatgtgga gttgattctg ctgtaagtga gagaatagct aaagctgcta
    36481 atgattcaga cgttcctata gtagcattta caaattctat aggaagtaac ccaactggag
    36541 aatttaaagg tttagttaca tatataggac aaaatgagga agaaacagga gcattaactg
    36601 gaaaaattgc taaaaactta ttaggagaaa ctggtgggaa agctgtatta atagaaggag
    36661 taccaggaac tactccacaa gtattaagaa aaaaaggtct tgaaaaagaa ttaaaagata
    36721 gtaatataga aatagtttat aatcaaactt caaattggga gaaagagcaa gctttaaaag
    36781 taacagaaga tttaattcaa aagaaaactg attttaatgt tataatatgt caagatgata
    36841 attctgctac aggtgcaggt caagctttaa aagatgctgg tcttaaggat aaagttaaag
    36901 tcataggttt gggtggaagt aaagatggat taaaagctat aactgatggg ttaatagatg
    36961 gtacaactta tatgtctgct gtagaagaag gtggattagt catagaaaag gcatctaaat
    37021 tccttaaagg tgaaaaaata gaacctgtta cacaaattaa acaagttgaa gttaacaaag
    37081 ataatatttc agagttcaag ggtgaatggt aaattgtcat agataataaa taagattttt
    37141 agagttactt tagaaactat ataaagtaac tcttttttta atttaattta gtgataaatt
    37201 attgaatagt atttctaaac aaataaaaaa ttaatataaa caaagactta attatactaa
    37261 attataagta aaactaatat aaacttatgg aaaaaaggaa aacgtctaaa gtattggaat
    37321 tactatagtg taggtgtggt attaaaaaaa cataaaaaaa cataaaaaaa ttgttgaaaa
    37381 aaatatagaa attttgtata attaaagatt tgaaaagtgc ggtatacaaa gccaattctt
    37441 ttgtatataa tatatataaa ttcagaaaaa ggaagggata tgtatggcca ggcggttatt
    37501 tttagaaagg attggactta tatttttagg ttcagcaata ttagcatttg gagtttataa
    37561 tttttattat ttaaacaaca taacagaagg tggagtgtta ggtattttac tactgttaaa
    37621 aaatttattc aatatacaac cagcaatagc aaatgttgta atagatggtt tactactttt
    37681 agtaggatat aaatttttcg gtaaaaaatt ccttatatat tcaatagtag cttctataac
    37741 tttttcagtg ttatatgact tatttgaagc tataggacct ctagtaccac aatcacaaaa
    37801 tatgctttta tcaactattt tggcaggtgt aactgttgga actggtgtag gtatagtagt
    37861 aaaagcaggt tgtgcatcag gaggagacga tgctttagca ttagtaattt ctaagactac
    37921 atctttgaac atagggcaag tttatttagc aacagatgtt attgtattgt tgttatcatt
    37981 attttatcta tcagcgtttg atatctttta ttctttgatt gctgttacaa ttagtggcaa
    38041 ggtaattgat tttatttatt atcatggtaa aagtttggat atggatataa gtaatgatat
    38101 agttccagaa tgttaatata aattaaataa ctttagattt tattaggggt ggcagatttg
    38161 ccaccttttt tgtgtctaaa tctttataga aagtataatc ccatctaggt ttttgtaaat
    38221 ctcaaatata gaattatgtt ggaatgactg ggaatacgat tgctagatgt gcattttaaa
    38281 aaccaaattt tatatttatg aaatactaat cattaatgga tgtttcttaa tcctatcttt
    38341 atacctaaaa tgataagata aatgaagtag caatacaaaa ctaaaatttg taaaagagat
    38401 taaattaaat ggaggagcta aaatgttgga tatagttatg atagcattgt tattcatagg
    38461 atttgtttcc atgaagttac ttgctgattg gtgtgggaaa caaactgaaa aaaaataatg
    38521 taatgtatgg agggattacc tatgtggttt ctggcagttg taattataat gttaataata
    38581 tatttggtat atgcgcttat taatcctgaa aaattttaaa cagtataagg ctaataagta
    38641 agttgaattg gggggaacaa tttttatggt gcagatagta atagtgttag cgatttttat
    38701 gattttagta atacctatgg gcaagtattt atatcatata gctactaatc aaaaaacatt
    38761 tggagataga ctatttgata aagtggacaa ttttatatat aaggtatgta gtatagataa
    38821 gaaaaaagaa atgaactgga aacagtatgc attagcttta ttatttacaa atgcagttat
    38881 ggtatttata ggatatatca tacttagaac tcaaagtatg catattttta atccaagtgg
    38941 aattaaaagt atggaacaag gactatcatt taatacaata ataagtttta tgacaaatac
    39001 aaatcttcaa cattattcag gtgaatcagg actttcatac tttagtcaga tgacagtgat
    39061 aatatatatg atgtttacat cagctgcaac aggatatgcg gctgctatgg catttgtaag
    39121 aggtctagtc ggtaaaaaga aaacattggg aaatttttat gtagatttaa ttaggataac
    39181 aacaagagta cttttaccag gagctttaat tattgggctt atattagtta cacaaggagt
    39241 accacaaact tttgctggga cagaaacagt gactactata gaaggaaagc ttcaagatat
    39301 agctagaggt cctgtggcag ctcttgaatc aataaaacat ttaggaacaa atggtggtgg
    39361 attctttggt tcaaattcat cacacccatt tgaaaatcca acaataatat caaatatagt
    39421 agaaatatta tctatgatga tattaccagg agcttgtgtt gttgcatttg gtcacatgat
    39481 aaagaataaa aaacaaggtt gggttgtgtt tggcgcaatg tcaatcatat ttttaatagg
    39541 tctagtagtg tgctttaaag cagaaagtgc aggaaaccct attttatcac aattagggct
    39601 taatcaaagt atgggaagta tggaaggtaa ggaagttaga tttggtatag cacaatcatc
    39661 attattcaca acagtgacta cttcctttac gacaggtaca gttaataata tgcatgatac
    39721 actgactcct ttaggaggat tagtaccact tttaaatatg atgctaaatg tagtatttgg
    39781 tggtaaaggc gtaggtctga tgaatatgtt gatgtacgct ataatagcag tattcttatg
    39841 tggattaatg gtaggaagaa caccagagtt tttaactaag aaaatcgaag gaaaagaaat
    39901 gaagcttatt gctttactta ttatattaca tccactttta atactaatgt tctctggtct
    39961 ttcagtagca ataccagcag gacttgaagg aatttcaaat cctggattcc atggattatc
    40021 acaggtattg tatgagtttg catcttcagc agctaataat ggttcaggat ttgaaggttt
    40081 aggagataac actatgtttt ggaatataac aacaggtata gttatgttct ttggaagata
    40141 tgtgtctatt atagtactac tagcaatatc aagtttgtta gcatctaaaa aagctgtaaa
    40201 tgaaagtata ggaacactta gaacggataa ttttacattt acaatagttc tagtacttgt
    40261 tgttttaatt gttggtgctt taacattttt cccagcctta gcgcttggac ctatttcgga
    40321 gcatctagtg ttgtggcact aaaaaattgg gagggagtaa agttttatgg aaactaaaaa
    40381 aacaaagttt attacaaaag atattttcaa aacttccatt atagaagctt ttaaaaaatt
    40441 aaatcctaaa tatatgatga aaaatccagt tatgtttgtt gtagaaattg gatttatagt
    40501 aacattgata cttacaatag tacctagttt gtttggtgat gaaggcaata acttaagagc
    40561 ttataatggg attgtaacaa taatcttatt tataacagtc ctttttgcaa attttgctga
    40621 agctgtagct gagggaagag gtaaagctca agcagataca cttaagaaaa ctagaaaaga
    40681 tacagtagca agggttatag atacgttagg aaatgaaaaa atgataagtg caagtgagct
    40741 aaaacttggt gatattgttt tagtaaatgc tggagaagtt attccaaatg atggagaagt
    40801 tatagagggc atagcttctg tagatgagtc agcaataact ggagaatctg cacctgtcat
    40861 gagagaatca ggtggagatt tcgcatctgt tacaggtggt actacagttg taagtgactg
    40921 gttaaagata agaataacaa caaaaccagg agaatcgttc ttagataaaa tgatagctct
    40981 tgtagaaggt gcttcaagac aaaaaacgcc aaatgagatt gctttaaata ctttgttagt
    41041 agggttaaca cttatattta tggttgtttt aatatctctt tatccaatgg caaactatgc
    41101 aggtgtaaag attccaattt ctactatgat agctcttttg gtatgtttaa taccaactac
    41161 tataggtgga ttgttgtcag ctattggtat tgcaggtatg gatagagtaa ctagatttaa
    41221 tgtaattgca atgtcaggaa aagcagtaga agcttgtgga gacgttgata ctatgatttt
    41281 ggataagaca ggtacgatta cttatgggaa tagacttgca tcagaattta tagcagttgg
    41341 aaatgcagat tcaaaagatt taataaatta ttctgttatg tgttcattaa aagatgatac
    41401 accagaagga aaatctatag ttgatttagg tatgaaatta ggttcaacag gtaagacaaa
    41461 agaggcagag gaagctgagt ttgtagaatt ttctgcacaa actagaatga gtggtattaa
    41521 cttagctgat ggtagagcta taagaaaagg tgcttatgat agcataataa aaagagtaaa
    41581 agaatctggt ggtacagtgc cagaagattt agaagaaaat gtaaatcgag tagctaaact
    41641 aggaggaaca cctttagtag tgtgtgtaga taatgagata tatggaatta tctacttaaa
    41701 agatacagta aaatcagggc ttgtagaaag atttgctaga cttcgagaga taggaataaa
    41761 gacaataatg tgtacagggg ataatccctt gacagcagct acaattgcaa gagaagctgg
    41821 agtagatgga tttattgcag aatgtaaacc agaagataaa atagatgcaa taaaaaaaga
    41881 acaattagag ggtaaaatag ttgcaatgac aggtgatgga acaaatgatg ctcctgcact
    41941 tgctcaggca gatgtaggtc ttgcaatgaa tagtggtaca acatctgcaa aagaagcagc
    42001 aaatatggta gatttagatt cagacccaac taagatattg gaagttgttg aaataggaaa
    42061 acagctttta ataactcgtg gggctttgac aacatttagt atagcaaatg atgttgcaaa
    42121 atattttgcg attataccag ctatatttac tcttgcaata cctcaaatgg agatattaaa
    42181 tgtaatgaaa ctttctacgt catttagtgc gatattatca gctttaatat ttaatgctat
    42241 aattatccca tgtttaatac ctcttgcaat gaagggagta aaatatagac ctatgaaatc
    42301 acaagctatg ttaatgagaa atatgcttat atttggtctt ggtggagtta tagtaccatt
    42361 tttaggaata aaattaattg atttattgat tacaccttta gtaatggttc ttaatttagg
    42421 ttaataaatg gaggatataa aaatgaaaac attaaagcca gcattacttg taagtattgt
    42481 attgttagtt gtttgtggac ttgtatatcc acttgttttg acaggtgtaa gtcaagttgc
    42541 atttaaagat aaagccaatg gaagtatgat agaagtaaat ggtgtaaaag taggttcaga
    42601 acttattggt caaagcttta cagatgcgag attctttaag ggaagagtat catcagtaaa
    42661 ctataatact tatacaaaag aagatttagt accagataaa gatggaaata catcttatgg
    42721 aggtgtatca tcagggtctt ttaattatgg agctacaaat ccagaacttc atgatagagt
    42781 acaaaaagat atagaaaagt ttttaaagga taatccaact gtcaaaagag aggatatacc
    42841 aacagattta cttacagcat caggttcagg acttgaccct aatataagtc cagcctctgc
    42901 aaaaattcag ataccagcta tagcaaaagc gtctggaata tcagaaagta aattacaaaa
    42961 aattgtagat gataatactt ccaaaaaact ttttggagtt ttaggtgaag atagagttaa
    43021 tgtattaaaa gttaatgtag aggtcgcaaa aatattaggt cttatatagt attttgtgga
    43081 attttagcaa caaaatatta ctcaagattt aatttaaagg ttaaatttta aagagtgaaa
    43141 atcgataatg attttcactt ttttatttga atattactaa tatttggaat aaagtgattg
    43201 taagtataaa atccaaaaaa aggttgcatt atcaaaatga taatactata atatatttat
    43261 cgttatcaat ttgataatag atttggacat ataggaggaa atagtatgtt atataataac
    43321 gcattagagt tgataggaaa cacacctgta gtaaggctga ataatttagg ctgtactaat
    43381 gggtgttcta acatatatgt aaaattagaa aaagtaaatc cagcaggaag tataaaagat
    43441 agagctgttt atgaaatgtt agagggctta gagcaaagag gagaattaaa aaagggagat
    43501 gtactagttg aagcaactag tggaaatact ggtattgcat tatctatgat aggaaaatta
    43561 aaaggatatg aagtagtaat cgtaatgccc gaaacaatga gcgttgagag aagagattta
    43621 atgaaggctt atggagcaaa tttggtgctt acagatggta aagctggaat ggcaggttcc
    43681 atagaaaaag caaatgaact tttaaaagaa aatccaaact ataaaagctt aaaacaattt
    43741 gaaaataaag ataatccaaa tgcacattat aaaacaacag cagttgaaat tatgaatgat
    43801 gtcaaagatt tagatatatt cgtatgtgga gttggaacag gtggtacttt aataggaact
    43861 gcaagatatt taaaagaaca aaatccaaat ataagagtaa ttgcacttga accaaaaaaa
    43921 tctccagcta tatcagaaaa caagactgga cctcataaaa tacaaggaat aggtgctaac
    43981 tttgttcctg aaaattatga ttctagtgta gttgatgaag taatattagt agatgatgaa
    44041 gatgcatttg atacagttaa attacttgca gcaaaagagg gaattttagt agggatatct
    44101 tctggagcaa atatatttgg tgctatagag atggcaaaaa agtatccaga taaaaagata
    44161 gttacagtag ctccagatgg agttgataag tatatgtcta tgggaatatt ttaataattt
    44221 tatttgaggg agtgaatact ttgtttaaga aaataaataa agatattgaa tatataatga
    44281 agaatgatcc agcagctagg tctaagatag aagttttttt actttatcca tcagttcatg
    44341 caatgataat gcataggatg gctcatgctc tttataagaa gaagaagctt tttacagcaa
    44401 gattaatatc ccagatatcc agatttatga ctggcataga aatacatcct ggtgctaaaa
    44461 tgggggaagg tatattgata gaccatggaa tgggtgttgt aattggagag acagcggaag
    44521 taggtaatag ggtgacaata tatcaaggag ctactttagg agctacagga aaagatactg
    44581 gaaagagaca tccaactgtt ggtgatgatg tattaattgg tgctggaaca aaaatactag
    44641 gacctttaaa tataggttca aattcaaaaa ttggtgcaaa ttctgttgtt gttaaggatg
    44701 tacctaatgg tgcaacagtt gttggtatac cagcaaaaat agtaaaaata agaaacctag
    44761 aacctgtaaa aaagaataaa aaagaagtgt cctatgagta tgatgaatta gataatgtat
    44821 attatatata ggatttatat aactataaaa taaactttat aaaaattatt ttaaaaggta
    44881 tttttttcaa atttgaggac ataataaaaa agaactgaat gttacagttt tggttcatca
    44941 atctttgata aataagattt taaatttgta gcgtctagga ggattttatt atgaaagcaa
    45001 atgttaatca agatacttgt ataggttgtg gattatgtcc atcaatatgt ccagaagtat
    45061 ttgatatgaa ggatgatggg aaatcacatg ttatagttga tgaagtacca agtgatgctg
    45121 aagaatcagc agcagaagct agagaaagct gtccagttga tgcaatagat gtgcattaat
    45181 aaaataaatg cctataaaaa ttgaattttc aatttttata ggcatttgtc ttttattcag
    45241 ttagtatttc aacacctgtt tctgtaacta aaaccataca ttcccattga gcagataatt
    45301 taccatcttt tgtataaata gtccaattat tttcttcatc aacaaaaatt tcgtgagttc
    45361 cttcatttac cataggttct attgtgaata tcatcccagg aaccaatagc atctcagtcc
    45421 cttttttaga tacataactt acaaaagggt cttcatgaaa gcttaatcca actccatgac
    45481 cacctatttc tctaacaaca gagtatccat ttttctttgc ataagagttg atagcatcac
    45541 ctatatctcc taaaaatccc catggtttgg caacttcaag acctttttgg acacattcct
    45601 ttgtaacttg aactaatttt tttgcatttt cattaacatt acctatcata aacattcttg
    45661 aagcatctga atagtatcca ttaaaaatag tagaaacatc tacatttata atatctccat
    45721 cttttaagat aatgtcctta cttggaatac catgacaaac ttcattattt actgatgtac
    45781 atacactttt aggaaatcca ttatagtcta gtggagcagg gatagctcca ttttgtagtg
    45841 taaagtcatg aaccaactta tttatatctt ctgtagacat tccaatttcg attttatctg
    45901 aaatataatc caaaattttt gtgtttatta cagcactttt ttttatttcc tcaatttgac
    45961 tttcattttt tataatatca tgagttggta caatatgacc taacttagca aatgaatcta
    46021 ttttattatc catatccata tgacattttt tatatttttt agtacttcca caccaacagc
    46081 tatcatttct atttaacttc ataacttttc atccttcttt tattatttat ttaaattatg
    46141 ggttacataa actattttaa aacataacca ttataaatat tataacataa atactaagaa
    46201 atcctttata gatagtgatt tattcaaagt ataaattatt atatggttat tgttgatgaa
    46261 aagaggatag ttaaatgtat tattggatat tagcgcactt aatagtttag ttcaagtttt
    46321 tcaagaaaat catatgttag aggacaattt tcctttaaag tattttttga atcttcatca
    46381 agataataat gtacaaatga ttctgcaaaa aattcttcta taaacttgct tatatatgca
    46441 tgtgcaactt gcttagggtc ataaagctta tatctttcct gagcaaatac ttccataaat
    46501 tcttcagaat ttgatttttt atttaaaaca tttttatcta tagcatgtgc agtttcatgt
    46561 aattctaaat tttttgatga atgtacattt gcatagctag gattactata acctatttta
    46621 gccactatag aattatttct atatatacca ggtacatctt tccaagtctt gcctgatctt
    46681 acccaacatg gaggtaattg atattttaaa tcactaaatt ctggttcatc agttagattt
    46741 gaatttatta attttatttt tatatccttt tctttaagtg tctgtaaata tttaatattt
    46801 atttttgcaa gtctgctttt catattttca acttcatctt tatcataatt caagtcatca
    46861 ggaagtataa gcatttgttc aattatatct atactactgt cttttatatt ttcagattct
    46921 tcatcaaatt tatattgaag agtattattt tcatagatag ttatatttgt attattttct
    46981 atagaataag agtgaatatc ctctaaatct aaaatattca tttgttgtaa aatatgtttt
    47041 gtttcgccac tccatactac taaagaaagt gaattaaaat taattagtgg attttctttt
    47101 gcttttatat cattgcttat atctacatca aatacatatt caattgagtc ttctatgtta
    47161 agactatctg tattcttttt aatatataaa tacgcttgtt ttaagtctat ataaatctca
    47221 cctaaagatt cgtcataaaa ttctgataaa ttattagtaa agagttcttc acttaaaagt
    47281 tcatttttat cagttatact ttcaattaaa atattaattt cttttatcat atcgtaaata
    47341 cttatttttc tatttgcttt ttcactagat acattgcatg gcgcaagagc aatgcaataa
    47401 aaatcttttt cttttttata aaaggcttta agtgattttg ataaaagtgt aaagtctaca
    47461 cctatctgta agttgacctt aagtgagtca ttttgtaata attcagtatt atttctttta
    47521 gctgtattta aattgttttc cataatcatt gccccctttt tattagggta tcataatttt
    47581 tttatatgtg tcaatttgtt tttttgaaaa ttataatttt tttgaaaatt ttatttttta
    47641 taatttgtga attttttatt atagaaataa atatgttatt attatgtcaa aatttagcat
    47701 tagattcatg tagagtatgt tttatacata tattataatt aattctttga agattgcata
    47761 ttcaaaatac acaccatttt ttttaattct ataatataat atttatatat ttaatgttag
    47821 gatgtgattt aaggtgtatt ttacatatat tataagatgt aaagatgatt ctctatacac
    47881 tggatatacc agtaatatag ttagaagaat gaatgaacat aagttaggaa ttaattcaaa
    47941 atacacaaga gcaaagggat ttgaaaaact agaagtttat tttgtgacca acacaaaaag
    48001 caatgctatg aagttagaat actatataaa aaaattaact agaaataaaa aattgtctat
    48061 aataaaaaac ccaagtatac ttataaattt aattgataat aaagaagatt atattattgg
    48121 taagaaaata gaacaattaa cataaatatt tatagtaaca ttataatggg tgcatatggt
    48181 gtaaaaactt gacaataaat actattgtaa tataatgaag ttataaatat agtaaattaa
    48241 aatagctagg ggagccaaga gctgagaggt ataccgaccc ttacacctga tctggataat
    48301 accagcgtag gaaagcttag taaaaattca agtatgtatt atttgtgata tatgctattt
    48361 ctaagctata cctattgtag gtatagcttt tttaattaag ttatttaaat taaactttag
    48421 gaggcaacaa tgagtaatta taaaatacca acacttacaa tagccggctc tgattcatct
    48481 ggaggagctg ggattcaggc tgatttgaaa acgtttagtg caattggtac ttatggtatg
    48541 agtgtaataa cagctataac agcacagaat actaaaggag tttttgctgt agaggattta
    48601 aataagaaaa taataaaaaa acaaatagag gcagtctttg aagatatacc tccaagagct
    48661 gtaaaaatag gaatggtttc aagtcctgag attatacttg aaatagttga gaatctaaag
    48721 aaatacaatc caaaatattt ggttgtggac ccagtaatga tatctaaaag tggatattat
    48781 ctattaaagc cagaggcaaa agaaaactta attaagtatt tgataccact tgcatacata
    48841 attacaccaa atataccaga ggcagaagaa ataacaggaa taaaaataca taatgtagat
    48901 gatatgaaga gagtaggaga agaaatacta caacttggac ctaaatttgt acttatgaaa
    48961 ggaggtcatt tagatggaga agcagtggat attttagttg gtaaaaatat atttaaagtt
    49021 tataaaagtg aaagaattga taaaaaaaat acacatggaa cagggtgcac attatcttct
    49081 gcaataacat cttatctagc attaggatat gaaataacag aagcagtaaa tctaagtaaa
    49141 atctatataa cagaagcaat aaaaagaagt tttgatatag gtcatggagt aggaccagta
    49201 catcattttt ataaatttga gtaataaaaa aatattggag gtaaaatatg tataatttaa
    49261 ttaaagatgt aaaaaaatta aatccattgg taattcatta tactaataat gtaactataa
    49321 atgattgtgc taatgtaact ttagctgtag gagcaagtcc tcttatgagt ttttcatatg
    49381 aggaagtaga agaaatggta agtgtagcaa actctgtagt aataaatata ggaactatga
    49441 actcaaatat gttggattta ttcctattag ctggaaaagc agcaaataag tacaataaac
    49501 ctgttgtttt ggacccagtt ggagtatttg caagcaaagc gagagcagaa cttacaagta
    49561 gacttttaaa tgaagtcaaa tttagtgttg ttaaagggaa tgtatcagaa ataaagttta
    49621 taggtggctt taatgtaaag ggtaaaggtg tagattcatt tgatgaagaa gaagattcta
    49681 ctgagataat aagaaagatt gctgaaaagc ttgaatgtgt agttgtagca actggaaaaa
    49741 tagatataat aactaatggt aaaggtacat ataaaataaa caatggaact gataaattaa
    49801 aaggaattac aggaactgga tgtatgactg ctagcttgat tgcaagtttt atggctgtta
    49861 ctgagaatat attagaagca gcaacaatgg gtgttcttac aatgtcttta agtggggaat
    49921 tagctaattt aaataatcca ccaataggta catttaaaga aaatcttatg aatgctattt
    49981 atcaaatgga tatagatact ttaagtaaaa attctaatat tgaattttta aattaataaa
    50041 gagtaaggga gacttaaaat gattgataaa gaatctttaa aaaaatgttt aaagttgtat
    50101 ttagtaactg actctgaaat gcttaaaggt agagactttt ataaatgtct tgaagatgca
    50161 atatcttctg gaataacaac tgtacaactt agagaaaaaa atgcttctgg aagagagttt
    50221 ttaagaaaag ctatgaaact tagagaaata actaaaagat atggtgttaa gtttataata
    50281 aatgatagag tggatatagc acttatttgt gatgcagatg gagtacatgt aggacagagt
    50341 gacattgatg ttagagaggt tagaaagctt ataggaaaca ataaaattct aggagtatca
    50401 gcaagaactt tagaagaagc tatttgtgca aaaaatgatg gcgcagacta tttaggtgta
    50461 gggtctatat tcacaacatc tactaaatta gatgcaaaat cagcttcgtt tgaaactgtt
    50521 aaagaaatta aagaaaaagt agacatgcca tttgtactaa ttggaggtat aaatttagac
    50581 aatatagata aacttaaatg tttagaaagt gacgggtatg ctataatatc agctatatta
    50641 aaagctgaag atatctctaa agaagtggaa aagtggacat taaaaatata aaattggctc
    50701 tggtagtgaa aaaaatggaa aaatttgata taatctaaga gtaaaattta agcaattata
    50761 aatgataaac ttttctacat atacttttta atcaagatat atgtagattt ggttaaaaag
    50821 gagctataaa tgattaggat attgttagtg tgtgttggag gcatgtcttc taccctattg
    50881 gtaaataaaa tggagaagga tgcaaaaaaa agaaatatag actgtaaaat atgggcagtt
    50941 ggagaaggag atataaaatc ggagcttgat aactttgata ttttacttct tggaccacaa
    51001 cttagattta tgcttgatga cgtaaagtct atagttggag atagagctcc agtatctata
    51061 atagatatgg taaactatgg cacttgtaat ggtcatgctg tgttaaattc ggttttagaa
    51121 atattaaaat aaataataaa agtggattct atataataga gtccattttt attatttgtt
    51181 attaataggc ttaaaactta gcttaccatt tcttttttta atctccacat agcaagtgaa
    51241 aaacttgaac agcacaatat taaaaatagt aggataaaag gcagtatatc tccaataaga
    51301 atagtctcac caaatattaa gctacgtaaa gcgttaataa catgggtaaa tggattgagc
    51361 atcactgcta cttttaatcc tccagataaa ctttctatag ggaaaagagc tgaacttaaa
    51421 aagaaaatag gaagtacaat tgcagtcata attgtttcat aaactatttc atttggcaat
    51481 aataaactta tagaataagc tagacttgat aaaaagaagg ctgtcataaa tatcagtact
    51541 atcataagta ggattccacc aatgccagat tctatcctta ctgagaagaa tatacttact
    51601 atacacaaaa tagttacttc tatgaaagaa actaagatag cttctaataa ttgaccaagg
    51661 acgattgaat acctgctgat aggtgcaatt aagactctat agaaacttcc actgttcttc
    51721 attataaagt ttataatacc accactacta caagaagaaa acacaaccag tacaatgata
    51781 cctggtagta taaaagcagt ataattattg atatttatat tattcatagt ttggtttgca
    51841 atagaactat aaagcacaag ccaaagaaaa ggttgtaata tagtaactac aaatgagatt
    51901 ggatttttaa aacgccactt catactacgc cataagatat ttaacattcc cattcctcct
    51961 ttaagctttt tttagaactt gtaagtgcta aaaatacatc ttcgagactt ggctctacaa
    52021 tttctattgc atcaaattct atatcatgtt ctaaaagcca tttatttatt aaagtaaaag
    52081 cagttctact atcgtttaca cttataaaaa ttgaattttc tcgtacgctc ataaacttta
    52141 ctaaaccagt acttttaatt gaatctttat atttttttat atcttcagta ttatgaaaag
    52201 ttatacgtag tatattttgt ctaatgtaac tacgtaaact tgatggtgtt ccttgtgcta
    52261 aatcctttcc attcttcata atacaaatat tatcactcaa ttgttcagct tcttcaagat
    52321 aatgagtagt tagaaatata gttgtgccat actcatctct aatttttaat aacatatccc
    52381 acatagattt tcttgaatcg acatccatac caacagttgg ttcatctaaa aataggattt
    52441 taggagatga taccatattc atagctatgt ccaatctacg ttttacaccc cctgaatatg
    52501 atgaagtagg atattttaag taactagata agtcaaactt atcaattaaa gagtctattc
    52561 tttgttttgc aatctgtggt tctactttat acattttact ttgaaatacc atattttcca
    52621 taagggataa atgttcatca atagagattt gctgtgctac acaagcaatt tgagtacgta
    52681 tccaagaaga attatctact aaatcttttc caaacatttt tacatttcca gatgtaggtt
    52741 tgtaaaaggt agtcaaaata tttataagag aagatttacc agcaccatta ggaccaagta
    52801 aagaaaaaat ttctccagca tttacattaa agcttagacc atttaaagct ttcacaccat
    52861 ttttatattc ttttactaaa ttgtctattt caatcgctaa catatattat tcctcctctt
    52921 taagtggaaa tattatctct gtaatatagt tatctgggtt tccttttatc aacatacctg
    52981 gtcctttaat aaatacttct ctccaaggag tttgtatttt tatattattt tcatgaatat
    53041 atttaaatat catttcatac gtttttggca aattactata agagcctgtg tgagtaaaaa
    53101 ataaagcttt tgttttagga aattcttttg tagttatacc catacgattt ggagtttcag
    53161 cagttggaac acataattcc atttctgcaa caccagtttt ttggtcaaca tcatagtagc
    53221 aaaagaatgg tgcaccgtta gactttcctt ttatagcttt aaatacattt ggaaaatact
    53281 ttgttgcctc agtcataggt ccattataac gcatggtagc cactgtgact gattcgatat
    53341 ttttgatttc aattttgcaa ttcatttgtt tacctcctaa tgtgtgttca tattcttact
    53401 agatttttaa gcattaatac atataagatt ttgattagtt gaaaacatat aatcttatct
    53461 cgtttgcttg ttttcaagtc taacataaca taaaatatat atcctctcat ttgatgctcc
    53521 attttgtaaa gttattattt aagctttact ttagattttt tagcaaatta aaataaagca
    53581 atttataaaa taagtattta caaattaagt gtattaatcg tataatacac ttaataggat
    53641 actagataaa gtctgaaata aaggcgtatt tttttaaagt aagtgtacta tcgtattaat
    53701 acactagaat aggaggaatc atgaattttg aattagataa tacaactccc atttatttgc
    53761 aaatagtaaa gtatataaag agacaaatag taacaggaga gttgaaacca ggagagacaa
    53821 taccatcaag aagagaaatg gcacttaatc taaaagtaaa cttaaataca gttcaaagag
    53881 catataagga gatgggtgat atgaatataa taaatacttt taaaaattat caaagcagtg
    53941 taacagttga tgaaaatata ttaaaaaatt taaaattaga gcttataaat gaatcactat
    54001 ctgtttttat agaagatatg aaagcaataa atgttagcaa agaagaagtt ttaaaaataa
    54061 tagaagataa gtattagttt tttaacaaat agaaaactta gtttagaaag tttatttata
    54121 agcatattgg atgtggagga tagtaaatat gatagaaata aagaatgtca gcaaaacata
    54181 taaaaggatg caagggctta aaataagaaa gatagaagct ttaaaaaatg taagttttaa
    54241 tatagaaaag ggaaaaataa ctgcattact tggtataaat ggtgttggta aatcaaccat
    54301 gttaaaagca atagcaggac ttataaacat tgatagtgga gaaattcgta tagatggaga
    54361 aaaaataaat gaaaaagtat acaataaatt ggcttttgtt ccagatgtac aatctcactt
    54421 tagcaataca acaataaaag aaacatttga atttatggaa attttttatt caaagtggaa
    54481 taaagaaaaa tcaaaagaaa tgatggacat atttaaattg gatgaagatg aaataattga
    54541 taacttgtca aaaggaaaca ttgcaagagt aaaattaata cttggatttt gccaagaccc
    54601 agagtacata cttcttgatg aaccatttac aggaattgat ttgtttaaga gggaagagtt
    54661 tataggagta atagcgcaat atatggagga aaatcaagcc attatcataa ctactcatga
    54721 gatagtagag atagaaagtt tagttgatga ggttgtaatt cttgatgaag ggcagattat
    54781 aacttcattt aatgcagaag aattaagaga aagagaaggt aaatctatat tagataagat
    54841 gagggaggta tataagaatg aataaacttc tatatctaat aaatgtagat ttgagaagaa
    54901 gcaataagtt ttatatagct tatatatcat ttttttcatt aattacactt agtttaaatt
    54961 tattcgaaat taataaattt aaaaattcta aatttattat gaacttagca atagaagatt
    55021 ttggaggtat attttatgga ctagggatac ttagtaatct tggttttatc caaagtatat
    55081 tgttctttgg gatattggga ctttttatat actatatttt tatgtggaaa agagagttct
    55141 tttcaaataa tcaaagtatt tatacactta tgatgttacc tcaaaataag tttaagattt
    55201 gtatatctaa atcaatagcc ttgataacaa tgatatatgg ttttttgata agtcagacag
    55261 caacattatt tatatgtaaa tatatattta atttcatttt tagaaatatg cctataatca
    55321 atatgaactt tgccaaagat ttatattatt taaacttgaa gagtatacca attgatttta
    55381 tctcttttat ggctatttat gtatttttac tacttatagc tatttctatt gtaaattgct
    55441 gtatattatt tatcttttcc tatataaata aattttatgt aattttaatc actttgctga
    55501 ttatagcagg cttatatttt tcaacaatat atataaatat aatcacttta tttagaaaat
    55561 attataatct tataggattt caatttaaca atttagtagc agggttatta agttttttaa
    55621 ttataatgtt tattctaaat ggaatatctt atttgctaat taaaaagaaa gtatctcttt
    55681 aaggaggggt ggtatgaaat taaaatctaa aatatctata tttattatat ttttgacttt
    55741 agcaatatat tttagtatag gtttattaaa taatgaagat aatttaaaga taaaaactat
    55801 aaaaggtgac tctaatgaat tagaccatat aaacataaat tttggatatg ctttgaatgg
    55861 atatataaat tcatattata caataaatgg aagtaacaaa accaaaaaga ctgaattaat
    55921 aaaactacaa aaggatagta agaacacaaa taatttaatt gatgtatcta gtggttcaga
    55981 tttaattgct catgttggat ttgaaatgaa tgaggagtca aaagatataa actttgtgat
    56041 agatgagaaa aaagaagatt tagaaaaagg tgtatttcca tcagaaggta gtgataaaga
    56101 aattgaatat gaaaaattag atatgaataa ttttacaagg acaagatata aagtagataa
    56161 aagtacagat gattattatg caaagatact agcatcaaaa agatataaaa aatacttata
    56221 tgtatctgta ctttatgcta caagtgattc atcagaaaat aacaaagaat attcaagtac
    56281 tatagatata ataaaaatta atccagaaaa taaaaaaatc aagactataa aaagtataaa
    56341 tttaagtaat tatttttccg aaaaatacca aaacatagag aacataggtg taagttatgc
    56401 tagcaaatat gaggataaga tgtatatttt agtaaatgta ctaaatacaa acataggtga
    56461 taatagtaat tctaaatttc atttacttga gtataatctt gaagaaaata aggaaagcat
    56521 aatggaatta aaaactgata gcaaaatggc tttagcaaaa gcaaagttag aagctaataa
    56581 attatatatg gtaatgtcga atgattatga taatagttca attacagagt taaatttact
    56641 tacctataat atattagata aaagcttaga tgaagataaa tatgttttag ataatagaga
    56701 aaaaagagga attagaagta ttgaaattga ttcaaataaa atcgttgcat atacatcaag
    56761 gctaaactct atattatctt gggatgataa atttatgcta tatataataa ataaaaattc
    56821 taaggaaatt gtatatgaag gagaattaca atttaacagt tcaaatagta tgttagaatt
    56881 tgaaataaag taggtgagtt gatgaaagat ttaataagat tattcgaatt tgaattaaaa
    56941 agaaatataa agaattatat atttataata atttgttgtt gttcttttgt aattttaaac
    57001 atagtaaaaa atttaaatga ctataattac ataatagaaa atgcagttaa aagtgaacag
    57061 ttaacaaaaa tcggaaatac gatagaaacg gtaaatgtta caggtttctc aagctttaga
    57121 aatataatgg gttctacaga atcgtggttt atgtttggta taattgcatg tatttgctat
    57181 gcttttttta tttggtatag agactttaat ggtagaagta aatctatata tacacttata
    57241 atgctaccta aaaatagaat caatatttat atatctaaat tattaaatat attatttcta
    57301 gtgtatagct atacagtagt acttacaata tctttgttta ttgcaagtaa gttgttgcct
    57361 agacatatgc ttggtaatgt cacaaactat ggttttgtac aagaaacaat atatgagtta
    57421 aaaatgttac ttccatatag ctttgaaata ctatttgtag agtatatatt tttattgata
    57481 ggatttgtat ctgtagtatt tacatcaatt cttataaata aatctattta taaagtaagc
    57541 actttaataa gttttctatt tttagttata gaaattttag tgtttataat ttctatagaa
    57601 tttattatac cttacgaata cagtgatgta tttactgttc tatattcaag tataaacata
    57661 tttgtatgta gtcttatatc taataaactt ttgaagcaaa agattgactt ttaggaggga
    57721 tattatgaaa gcaaaaaata taggaaaata tacaatatgt tttataatag tatgtttttt
    57781 aatgattact atgaccttaa tattgagtta ttttaataag gataaactat ctgtaaaaac
    57841 tttaaaaggc aatgtaaaag atatagggag agtaagtgta gtatatacac ctgatataag
    57901 caaattttct tatgaggagg taattataag taaaaatgga gtaagaaata atacaaaccc
    57961 aaaatcaaga attagaaatg attatttaaa tattgacaaa aaatacagtg actttgttaa
    58021 tgataaagga aaagaattta tatataaaga tgatttgaat ataggagaag cgtatacctc
    58081 tgttaagtct gatttagtac aaggaatgaa tgctttaaat gattgtataa aggtttcaaa
    58141 taaaaattta aaaagtgggg tagaagaaaa ttttgatatt aatttaaata agtctgattt
    58201 taaagaaaat agttggaaaa ccttatttaa caaaatatat aaaggtaatt tatatatggt
    58261 aattagtgaa actaatattt caggttctaa agtcaataat atttgtatat taaagataga
    58321 tataaaaaat aagtcttata aattattaaa tagatttaat ttaggattac agaatggaga
    58381 acaacaaaat atcaatgaaa ttaaattttc tattggaagt aaattatact tagaaatagt
    58441 aaataataaa agtaaaaatg gatatattga aggacatgat agttactcta actttattat
    58501 atatgatata gaagataaca cttttaatat aagtaataaa tttatggata atctaaacaa
    58561 agaaaagaat gcaaatccaa aggagattgg cttattaagt aaaactaagc tagattatat
    58621 ggtaaaaaat aataaattga atcttatagt tacggatatg agtaattcca taactaatct
    58681 tgtttatgat attaattcta atgagattaa attagaagat tatgagatac ttgatttaaa
    58741 agtagattat aatgaagcat catctggagt taggaaagtt aagctactgg atgataagat
    58801 atattctatt tcaggtatta agaacagtat atcaggagaa ttaaaaggat ttaaaggggt
    58861 tgttgatgta gtcggaacta agccagtaaa atttcaagta tttgatatga aacttaaaaa
    58921 gaatatttat gaagctgagc taataaatgg agatataagt ttacaagaaa agttattttt
    58981 tgttgcaaat taagtgttag aattaatttt agaactttaa acttttatat gtatcacaga
    59041 taagtataag taagggagtg tctcaaaata aactttttag ctcatgagac acttttttta
    59101 tatgaaatca aatatatttt cattgtttag atttattttg ataatgaaaa aaagattatc
    59161 tttattttaa gaaagacatt tatttataat tatatacaaa aatatttttt aatgtttttg
    59221 tgtataatta taacaattta atttatatta atgcacttaa atttgaaata gaatgcttat
    59281 agtggtaaaa ttaattatta gaatattaga aataataaat aaataataca ttgtacttaa
    59341 aagggggaga aatttaagat gaacaaaaaa tatattaaag cagggaattt aattgatgga
    59401 accggaaata gtattatgca aaataagggt ataattattg atggagatac tatagttgaa
    59461 attgaagata taagagatga tttaaatgga tatgatgtgt acgattactt agataaaact
    59521 gttatgccag gcattatgaa ttgtcatgtt cacttgacaa tggagccagt tggaaatcca
    59581 aagatatatt atgataatgt atcagatgta gagcttgtag taaatacgat aaaacaactt
    59641 gatgcttatt tggattcagg agtaacatat attagaagct taggttgtcc caaatatgtg
    59701 gatgtacaac ttaaaaatct tatagacaaa gggtctatag atggtcctgg tatagttact
    59761 tcaggtccag ttatatgtat gacaggaggg catggatatt attttggtat tgaaagtgat
    59821 ggagtggatg aatgtagaaa atcagccaga acagttttaa aaaatggagt agattgtgta
    59881 aagataatgg caactggagg tgtaacaact gatggagttg agccagggtc accacaatta
    59941 actttggagg aaatgaaggc tgctgtagat gaagctataa aagcaggtaa aataactgcc
    60001 acacatgcac aaggaagaac tggtataaaa aatgcagtgc ttgcaggaat tacttctata
    60061 gaacatggtg tttatttaga tgatgaaata attgatttga tgcttgaaag aggaacttac
    60121 ttagttccaa ctgtggctgc accatatttt attctaaatg caggaaaaga aagtggaata
    60181 actgaagcta ctcttagaaa gtgtgagatt gttgccattc cacacaaaga gtcattccta
    60241 aaagcatata aaaaaggaat taaaattgca gttggttctg atgcaggaac ttcttataat
    60301 gaacatggaa aaacatatta tgaaatgaaa ctaatgtcag attatggtat ggataatatg
    60361 gatataatag ttgctgctac aaaaacagca tctgaacttt taagaattga taaaaattat
    60421 ggaactctag aaaaaggaaa gaaagcagat gttatagttg taaatggaaa tccagtagag
    60481 aatatagacg tacttgcaga tgtgttagca gtatttaaac ttggtaaaaa agttagataa
    60541 atagtataaa aataaaaaag agtgtctaaa gaaattttta aataattata gacactcttt
    60601 tttattagta ataattaaat cttttatact ttagaaaata gattctattt tatatttaga
    60661 tataactctt taaaatcatt cttatgcaat ataatctata gaatctacac atacaaagta
    60721 tattcttgaa tcattagaat ttccaagtac tataacatca tttttcttac ctagaacttc
    60781 aacaccagtt agaaccaaac taccagctac tacagtaact tgattattta tatttgacat
    60841 ataaagatta tctaggatac ctttattaca acagcaatca tcacaatcgc agttacaatt
    60901 acattctttg taatgttttt tatcaagata gtaacctaaa gatgctatga aatcttcata
    60961 atctgcaggt gtaatatcaa atgcaacagc tttaagatta cacaatgatg ctaaatctac
    61021 gtttgctaat ggagtagatg cagcaagtat aaaatcaatt atagcagcaa gtattgcatc
    61081 taatatagcc ccaaggtcta tcgtaggagg aataactgca tcaataagtg caattaattc
    61141 agctactcct ggtatagttc ctatagtatt aattaatccc tcaagagtta aagggactgg
    61201 aataggataa gatactctac cagctatatc tattaaatca cagttacaag cagaaaatct
    61261 ttcaaaagta ccatcaagtc cagataaatt atcttttgga ggagctgaaa gatctatacc
    61321 taccaaatta gcacctacta taaagaaatc tgagataaaa gcaaattgat taaagtttac
    61381 aaaaggtctt agagcatcat atcttagaag ttctaaggcc tctctcatac tttttttaca
    61441 acaacattgt tttattttag gttctttata tttatttgga taattatagt atctatcagc
    61501 tacttgataa ttttcatagt atctttcatt tgtatttgga taatcttcct catattcttg
    61561 tgtaaatcta aagtcctctc tacatttatt attttccact aaatacactc cttttatatt
    61621 ttaatttaaa tgtatagata attaagctta tctatatagt aatattatat ttataatgta
    61681 aaaaaatgtt acaaaagata ttataaacaa ataaaaaata ataatttgtc agataaactt
    61741 gtaaattttt atgaataatc cattctaaaa caaaaaactg tgtataaaaa aggtttatat
    61801 gttatattta tataaatata acatatgaac gagaaaggag tggtttgata aatgattgag
    61861 gtagataaat tgtgtaagtc ttttacaagg gttgtaaaag atgataagaa taaaagttca
    61921 ataagaaagc taaagaaagt aaaaactaaa aaagaagaat tttttgctgt aaataatgtt
    61981 tcatttaaag ttatggaagg tgaaattgtt ggaatattag gtccaaatgg tgcaggaaag
    62041 actacattac ttagaatgtt aggtggcata ttaacaccaa catcaggaag tataaatatt
    62101 tctggatatg attattcaat agatagaaat tcagcaaaaa aagaaatagg ttatctctct
    62161 ggaaatacaa aactatatgg aaggctttct ccgagagaac ttctaacaac ttttgcaagt
    62221 ctatatgaga tgtcaaaaga agatatagaa gaatcaattg aaaatattgt aaaaataatg
    62281 gatatgagtg aatttataga taatagaatt gaaaatctat caactggtca gactcaaaga
    62341 acatctatag ctcgttgttt aatacattct ccaaaagtat atatttttga tgagccaaca
    62401 ctaggtctgg atgttttaag tagtgcatct attatagatt ttatgaagaa tgaaaaacta
    62461 aggggaaaaa ctgttcttta ctcaactcat tatatggaag aagcagaaac tttatgtgac
    62521 aagattttta tgattcacaa tgggaaaata attgcatcag gaacaccaca gagtttaaaa
    62581 gaggaatctg gagtaaataa tttgagagat gtattcatag aacttgccag aaaggaggac
    62641 atttaaatga gaagtaagat tgtaaaatac atatttaaaa aagaaatgtt agacatattg
    62701 agagataaaa agactttatt tatgatgatt gttgtgccac ttcttttata tcctttactt
    62761 atgttattaa tgattcaagt aatgaatatg tctgcaagta gtatgaatag taaggacatt
    62821 aatatagctt ttaataataa acctaataag gttttagtat ccatgattga agatgataat
    62881 ttagaaaaag atactaaaaa tacttcaaat gataaaggca aaataagagt gttagatgta
    62941 gataactata aaaagtcatt agaagaaaat aaaattgatg cttatataaa gatggaagaa
    63001 aaaaaatcat ctataaatta tcaaatatat ataaattctt ctcaagaaaa ttcttctaat
    63061 gcactaaaaa gaattgaaac tgttttggat aaatataaaa gatttaccat agaaaaaact
    63121 ttatctgaaa aaggattaga tgtacaagct actttagaac caataacata tagcactgta
    63181 gatgttgcaa agacggaaga agtggcagga tactttttag gtcagatact tccatttata
    63241 ttaataatag gagttttact aggttcaata tatccagcaa tagatgttat ggctggagaa
    63301 aaggaaagag gtacattaga aacattattt acactcccaa tatcaaactt agaactggtt
    63361 atgggaaaat atatggcagt ttctcttagt gcaatagtaa cagcaatttt aaacatttta
    63421 tctatgggac tgactatggc atttatttta gttagtggag gtattagtag ccaatttggt
    63481 tttggaaact ttaattatgg agatttagtc ttaccaatta taactacttt aatttgcata
    63541 tgtctttttt caatggtagt ttcagcaata agtatgtgtg tatgttcatt agcaaaaagt
    63601 tttaaagatg ctcaaaacta cataacacct cttatgttaa ttattatgat accatcatat
    63661 gtttctatga taccaaatat agtattagat agagtaacag ctgtaatacc tgtagtaaat
    63721 atatctttac ttataaaaag tgtattatct tttaaaagcg atttaaattt aatagcctta
    63781 gttttagcat caaatgtagc ctttgttata ttggcaatca tactactgtc taagatgttc
    63841 aattctgagg aaatactttt tggtaatagt aagagtttct ctttcttaga gaaaagaagt
    63901 aatataaaaa aagggtctat accaactgta agtgatggtg ttattttata tgcagtagga
    63961 ttaattctat tgatttatgt tggttcttta gttcaaatta aatttggtat ggcaggtatt
    64021 ataatgacac aattcatgat aatttcttta ccattattat ttgcatacta tattaaagct
    64081 gattttaaag aggtatttag cttgacagtt cctaaattaa agcatgtgtt tgggagcatt
    64141 gttctttgga ttggtggata catacttatt atgattataa cacagctaat tttattctta
    64201 tttcctcaaa atatggaagt tgctgaaagt ttaaataagg ctctttttat caaagatagt
    64261 gttttactga atttactagc aatagcttta ttaccagcta tatgtgaaga aatgtttttt
    64321 aggggattta tattttcttc atttagtaaa tcaaaagata aaaataagtc tatcaagcta
    64381 gctataatat gcagtggggt actatttggt attatgcata tggattttat tagaataatt
    64441 ccaacatcaa tattaggtat tatatttgca tatagtgtgt ataaatctgg ttcaatattt
    64501 gtatcaatat tattgcattt tttaaataat agtgtagcag ttgtgttaaa tcattataca
    64561 actggaaata taaccaactt atataaattt gttgaagttg atttttcgaa cttaaatgta
    64621 cttaaatttg ccttaattct tctaataagt cttatattaa ttatgttagg attaagagtt
    64681 ttagatagaa agtctttaag aaattaagta taaagttatt atatagatta aaatattaat
    64741 aagcactaaa
//
LOCUS       DAENCV010000004        62956 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAENCV010000004 DAENCV010000000
VERSION     DAENCV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.004"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            243..1118
                     /locus_tag="KQD82_000207"
     CDS             243..1118
                     /locus_tag="KQD82_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG4378713.1"
                     /translation="MNVYEKGNGVILERVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1136..2626
                     /gene="cls"
                     /locus_tag="KQD82_000208"
     CDS             1136..2626
                     /gene="cls"
                     /locus_tag="KQD82_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG4378714.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2819..3103
                     /locus_tag="KQD82_000209"
     CDS             2819..3103
                     /locus_tag="KQD82_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG4378715.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3136..4764
                     /gene="groL"
                     /locus_tag="KQD82_000210"
     CDS             3136..4764
                     /gene="groL"
                     /locus_tag="KQD82_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG4378716.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            5026..5766
                     /gene="larB"
                     /locus_tag="KQD82_000211"
     CDS             5026..5766
                     /gene="larB"
                     /locus_tag="KQD82_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG4378717.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEVSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5850..6584
                     /gene="larC"
                     /locus_tag="KQD82_000212"
     CDS             5850..6584
                     /gene="larC"
                     /locus_tag="KQD82_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG4378718.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKFKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6614..7033
                     /locus_tag="KQD82_000213"
     CDS             6614..7033
                     /locus_tag="KQD82_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG4378719.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7093..7194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7415..8950
                     /gene="guaA"
                     /locus_tag="KQD82_000214"
     CDS             7415..8950
                     /gene="guaA"
                     /locus_tag="KQD82_000214"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG4378720.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9489..10919
                     /locus_tag="KQD82_000215"
     CDS             9489..10919
                     /locus_tag="KQD82_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4378721.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(11067..11942)
                     /locus_tag="KQD82_000216"
     CDS             complement(11067..11942)
                     /locus_tag="KQD82_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4378722.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISHLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSKQALSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            12062..12625
                     /locus_tag="KQD82_000217"
     CDS             12062..12625
                     /locus_tag="KQD82_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG4378723.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVIAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            12622..13188
                     /locus_tag="KQD82_000218"
     CDS             12622..13188
                     /locus_tag="KQD82_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG4378724.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13317..13553)
                     /locus_tag="KQD82_000219"
     CDS             complement(13317..13553)
                     /locus_tag="KQD82_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378725.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13863..17291)
                     /gene="uvrA"
                     /locus_tag="KQD82_000220"
     CDS             complement(13863..17291)
                     /gene="uvrA"
                     /locus_tag="KQD82_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG4378726.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSIGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSKC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            17610..19844
                     /locus_tag="KQD82_000221"
     CDS             17610..19844
                     /locus_tag="KQD82_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4378727.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENLEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            20100..22130
                     /locus_tag="KQD82_000222"
     CDS             20100..22130
                     /locus_tag="KQD82_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4378728.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            22127..22579
                     /locus_tag="KQD82_000223"
     CDS             22127..22579
                     /locus_tag="KQD82_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4378729.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22581..23636
                     /locus_tag="KQD82_000224"
     CDS             22581..23636
                     /locus_tag="KQD82_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG4378730.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            23703..24011
                     /locus_tag="KQD82_000225"
     CDS             23703..24011
                     /locus_tag="KQD82_000225"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG4378731.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            24115..25380
                     /locus_tag="KQD82_000226"
     CDS             24115..25380
                     /locus_tag="KQD82_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG4378732.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            25468..26184
                     /locus_tag="KQD82_000227"
     CDS             25468..26184
                     /locus_tag="KQD82_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG4378733.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(26379..26672)
                     /locus_tag="KQD82_000228"
     CDS             complement(26379..26672)
                     /locus_tag="KQD82_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG4378734.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26678..26893)
                     /locus_tag="KQD82_000229"
                     /pseudo
     CDS             complement(26678..26893)
                     /locus_tag="KQD82_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27305..27817)
                     /locus_tag="KQD82_000230"
     CDS             complement(27305..27817)
                     /locus_tag="KQD82_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4378735.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            28267..28743
                     /gene="purE"
                     /locus_tag="KQD82_000231"
     CDS             28267..28743
                     /gene="purE"
                     /locus_tag="KQD82_000231"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG4378736.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            28749..29447
                     /locus_tag="KQD82_000232"
     CDS             28749..29447
                     /locus_tag="KQD82_000232"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG4378737.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            29459..30826
                     /gene="purF"
                     /locus_tag="KQD82_000233"
     CDS             29459..30826
                     /gene="purF"
                     /locus_tag="KQD82_000233"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG4378738.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            30820..31896
                     /locus_tag="KQD82_000234"
     CDS             30820..31896
                     /locus_tag="KQD82_000234"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG4378739.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            31890..32483
                     /locus_tag="KQD82_000235"
     CDS             31890..32483
                     /locus_tag="KQD82_000235"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG4378740.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKIKLQGRRVFINE"
     gene            32476..34008
                     /gene="purH"
                     /locus_tag="KQD82_000236"
     CDS             32476..34008
                     /gene="purH"
                     /locus_tag="KQD82_000236"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG4378741.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            34019..35269
                     /gene="purD"
                     /locus_tag="KQD82_000237"
     CDS             34019..35269
                     /gene="purD"
                     /locus_tag="KQD82_000237"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG4378742.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            35294..39100
                     /locus_tag="KQD82_000238"
     CDS             35294..39100
                     /locus_tag="KQD82_000238"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG4378743.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            39306..40178
                     /gene="rfbD"
                     /locus_tag="KQD82_000239"
     CDS             39306..40178
                     /gene="rfbD"
                     /locus_tag="KQD82_000239"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG4378744.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            40287..41156
                     /gene="rfbA"
                     /locus_tag="KQD82_000240"
     CDS             40287..41156
                     /gene="rfbA"
                     /locus_tag="KQD82_000240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG4378745.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGINITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            41205..41762
                     /gene="rfbC"
                     /locus_tag="KQD82_000241"
     CDS             41205..41762
                     /gene="rfbC"
                     /locus_tag="KQD82_000241"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG4378746.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            41800..42783
                     /gene="rfbB"
                     /locus_tag="KQD82_000242"
     CDS             41800..42783
                     /gene="rfbB"
                     /locus_tag="KQD82_000242"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG4378747.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVNSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKVILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            42813..43481
                     /locus_tag="KQD82_000243"
     CDS             42813..43481
                     /locus_tag="KQD82_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG4378748.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            43490..43918
                     /locus_tag="KQD82_000244"
     CDS             43490..43918
                     /locus_tag="KQD82_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG4378749.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            43942..44256
                     /locus_tag="KQD82_000245"
     CDS             43942..44256
                     /locus_tag="KQD82_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG4378750.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            44408..44683
                     /gene="flgM"
                     /locus_tag="KQD82_000246"
     CDS             44408..44683
                     /gene="flgM"
                     /locus_tag="KQD82_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG4378751.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            44688..45104
                     /locus_tag="KQD82_000247"
     CDS             44688..45104
                     /locus_tag="KQD82_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG4378752.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKKTTTYNSYGNIRK"
     gene            45122..46432
                     /gene="flgK"
                     /locus_tag="KQD82_000248"
     CDS             45122..46432
                     /gene="flgK"
                     /locus_tag="KQD82_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG4378753.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            46451..47383
                     /gene="flgL"
                     /locus_tag="KQD82_000249"
     CDS             46451..47383
                     /gene="flgL"
                     /locus_tag="KQD82_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG4378754.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            47399..47791
                     /locus_tag="KQD82_000250"
     CDS             47399..47791
                     /locus_tag="KQD82_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG4378755.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            47785..47997
                     /gene="csrA"
                     /locus_tag="KQD82_000251"
     CDS             47785..47997
                     /gene="csrA"
                     /locus_tag="KQD82_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG4378756.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            48019..48414
                     /gene="fliS"
                     /locus_tag="KQD82_000252"
     CDS             48019..48414
                     /gene="fliS"
                     /locus_tag="KQD82_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG4378757.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            48414..48770
                     /gene="fliS"
                     /locus_tag="KQD82_000253"
     CDS             48414..48770
                     /gene="fliS"
                     /locus_tag="KQD82_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG4378758.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            48791..50314
                     /gene="fliD"
                     /locus_tag="KQD82_000254"
     CDS             48791..50314
                     /gene="fliD"
                     /locus_tag="KQD82_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG4378759.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            50317..50652
                     /gene="fliT"
                     /locus_tag="KQD82_000255"
     CDS             50317..50652
                     /gene="fliT"
                     /locus_tag="KQD82_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG4378760.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            50754..51626
                     /locus_tag="KQD82_000256"
     CDS             50754..51626
                     /locus_tag="KQD82_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG4378761.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            51719..53860
                     /locus_tag="KQD82_000257"
     CDS             51719..53860
                     /locus_tag="KQD82_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG4378762.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            53884..55761
                     /locus_tag="KQD82_000258"
     CDS             53884..55761
                     /locus_tag="KQD82_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG4378763.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            55754..57868
                     /locus_tag="KQD82_000259"
     CDS             55754..57868
                     /locus_tag="KQD82_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG4378764.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            57877..58872
                     /locus_tag="KQD82_000260"
     CDS             57877..58872
                     /locus_tag="KQD82_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG4378765.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            58964..60196
                     /locus_tag="KQD82_000261"
     CDS             58964..60196
                     /locus_tag="KQD82_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG4378766.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            60216..61160
                     /locus_tag="KQD82_000262"
     CDS             60216..61160
                     /locus_tag="KQD82_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG4378767.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            61254..61661
                     /locus_tag="KQD82_000263"
     CDS             61254..61661
                     /locus_tag="KQD82_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG4378768.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            61748..62839
                     /locus_tag="KQD82_000264"
     CDS             61748..62839
                     /locus_tag="KQD82_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG4378769.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        24904 a         6622 c        11252 g        20178 t
ORIGIN      
        1 ctaataattt ataatataaa taaaaaatga atttcgataa gtttaagtgg gtataaactt
       61 atagaaattc attttttatt taaggtaaaa atttaaagta aaatattatt ttacataata
      121 ataaccttat aaattttgaa attacttatc tttgtgttaa aataagtaaa atgcacatat
      181 tagagctaag tttttaagta aataaacaaa atgaaaatat aaatattaag gagatttggt
      241 atatgaatgt ttatgaaaaa ggtaatggag ttatactaga aagagtaaca gattttgacc
      301 cagtacatat atttgagtgt ggtcaatgtt ttagatggca caaacaagaa gatggctcat
      361 acactggagt tgcaaagggg agaatattaa atgtcaaaaa agagaatgat aaaatatatt
      421 taaataatac taatttaaag gagtttaaca gcatttggta taattacttt gatttaggaa
      481 ctgattatac agaaataaag aataagctaa aaaatatgga tgaatattta aataaggcta
      541 ctgaatttgg atggggaata agaatattaa gacaagatgg atgggaaatg cttatatcat
      601 ttataatttc ctctaataat aggattccta tgatacaaag agcgatagaa aatttatcaa
      661 gaaagtttgg taagtatata ggtgaatatg aaggtaatga atactatgca ttcccaacac
      721 cagaagaact aaataaggcc tcacaagagg aaataagagc atgtcaaaca ggttttagag
      781 ataagtacat aaaaagtact acacaggcag ttatagagaa taatgatgag gtatctgaat
      841 atacaaattt aagtacagag gattgtagaa aagagctttt aaaatttaat ggagtaggac
      901 caaaagtatg tgattgtatt gcattatttg gtatgcaaaa atatgattct tttccagttg
      961 atgtttgggt taagagagtt atgcaagaat tttatataga tgaagacatg agtttaccta
     1021 aaatgagaac ttatggaatc gataaattta aagaaatgtc tggatttgct caacagtact
     1081 tattttacta tgcaagagaa ctcggtatag gaaaataaat taagggggag aaattatggg
     1141 agtaatagga acaatatttc tgttctattt aattatatca tatctagctg gagccataat
     1201 ttctgtgatt atattacttg aaaatagaga ccctgcaaaa acaatgtctt ggctattgat
     1261 gtttattata tttccaggtg taggtttaat gatatatgct atttcaggta gaaatattag
     1321 aaaaagaaaa ttatttaaga ctcaaaaact agctaataat ataaaagaaa agaaactatt
     1381 tgatacatta gaaaagataa cagaaatagt tgaattagaa aaagaatcta taaaacaaaa
     1441 taagctttta agagatgaag aggatggtag ttatagaaaa agggtaataa atatgttgtt
     1501 aaagactggt atgtttccct ttactaaaaa taataaagtg gatgtatttg tagatggaaa
     1561 tgaaaagttt aaaagactta tagaagatat aagagaagct aaagaccata ttcatttaga
     1621 gtactttatc atcaaagata gtgaaatagg tagggtgtta aaagaagaac ttataaaaaa
     1681 ggctaaagaa ggcattaaga taagaatttt atatgatgat gtaggatgct ggagattttg
     1741 gtttaataga aaatttttcc gagaaatgag ggaagtaggt atagaaattg cagcattttt
     1801 acctacaaaa tttcctataa taggagggaa attaaactat agaaatcaca gaaaaattgt
     1861 tgttattgat ggaataattg gatatacagg tggaataaat ataggagatg aatatttagg
     1921 caaaaatgat aagtttggat attggagaga tactcatatt aggattaagg gtatttctgt
     1981 atacatgctt caaatgacat ttctgattga ttggtattat acaacaaaag aagtattagt
     2041 gactaaaaat tatttcccaa gtgtagggaa tgttggtgaa agtatgatac aagttgttgc
     2101 gagtggtcca gacagtgact gggaagatat tcattatgct tatttttctg caatatgtca
     2161 ggctagaaaa aatgtttata tagaaactcc atattttatt ccagatgaaa gtctactaaa
     2221 agctataaag agtgctgctc ttagtggagt tgatgtgaga ataatatttc ctaaaattgc
     2281 agaccacaag atagtaaata tagcatctta ttcatatttt gaagaaatat taagagcagg
     2341 tggtaaagtt tatttatata ataaaggatt tatacattct aaagtcgtaa taatagatga
     2401 taaaattgct tctgcaggta cagctaatat ggatttaaga agttttatgc ttaactttga
     2461 ggtaaatgca tttatctatg atgaagaagt tattagggta atgacagatg atttctttga
     2521 ggatttaagt cactgtgaag aacttaattt ggaggtattt aagaatagaa atatcataca
     2581 aaaaatcaaa gaatctgtag caagattatt ttcaccaata ttataaaaaa ttattaaaaa
     2641 aggtattgaa aattatgtgt gattggtata ttataataat tgttagcact ctattagata
     2701 gagtgataat aaataaaaag tatatatata aatctacata tataagtagt attaatttaa
     2761 aaataactaa aaacataggc gaactagtct atacttaata ttttaggagg cgttaataat
     2821 gaaaataaga ccattagctg acagagtagt aattaaaaaa gtagaagcag aagaaaaaac
     2881 tgcaagtgga atagttttac caggagcagc taaagagcaa cctcaaatag ctgaagttgt
     2941 agaagttgga ccaggtggaa tagttgaagg gaaagaaata aaaatggaat tgacagtggg
     3001 agataaggtt atattccaaa aatattctgg aacagaagtt aagatagaag gacaagaata
     3061 cacaatacta agacagagtg atgtattagc tgttattgaa taaatataga ataaatttat
     3121 taggaggggt ttaaaatggc taaagaaatt aaattttcag aggaaacaag aagagcttta
     3181 gaagctggtg taaataaatt agcagataca gtaaaagtaa cattaggacc taaaggaaga
     3241 aatgttatat tagataaaaa atttggttct ccacttataa ctaatgatgg agtaactata
     3301 gcaaaagaga tagaactaga agatagattt gaaaatatgg gtgctcaatt agttaaagaa
     3361 gtagcaacta aaactaatga tgtagctgga gatggtacta caactgctac agttttagca
     3421 caagctataa taagagaagg tttaaagaat gtaacagcag gggctaaccc aatactttta
     3481 agaaaaggaa tacaaaaagc agtaacagta gcagtagaag aattaaaaaa tcaatcaaga
     3541 atagtagaaa cacaagaggc tatatctcaa gttgcttcta tatctgctgg tgatgaagaa
     3601 gttggaaaat taatagcaga agctatggaa atagtaggta aagatggagt tataactgtt
     3661 gaagagtctc aaactatgaa tactgaatta gatgctgttg aaggtatgca gtttgataga
     3721 ggatttgttt ctgcatatat ggttacagat gtagataaaa tggaagcagt tttaaatgac
     3781 ccatatatat taattactga taaaaaaata tctaacatac aagaattatt accagttctt
     3841 gaacaaatag ttcaacaagg taaaaagtta ttgataatag ctgaagatgt agaaggtgaa
     3901 gcattatcta cattagtagt aaataaatta agaggaacat ttgatgtagt agcagttaaa
     3961 gctccaggct ttggagatag aagaaaagaa atgcttcaag atatagcaat acttacaggt
     4021 gctcaagtaa tatcagaaga attaggttat gatttaaaag aagctgattt atctatgcta
     4081 ggtagagctt catctgttaa ggtaactaaa gaaagtacta caatagtaga tggttctgga
     4141 gataaaaaag ctatagaaga tagagtaact caaattaaac atcaagtaga gcaaactact
     4201 tcagattttg atagagagaa attaatggaa agattagcta aacttgctgg aggagtagct
     4261 gttgtaaaag ttggagctgc tacagaagta gagttaaaag aaagaaagtt aagaatagaa
     4321 gatgctctta atgcaactag agcagctgta gaagaaggaa tagtagctgg tggaggaact
     4381 gcttttgtta gtgttatacc agcaatagga acactaattg agagtttaga aggagaagtt
     4441 aaactaggtg ctcaaatagt taaaaaagcg ttagaagagc cattaagaca aatagctata
     4501 aatgctggtc ttgaaggtgc tgtaattgtg caaaatgttg ttaattctga agcagaaact
     4561 ggatttgacg cattgaatga aaaatatgta aatatgatag aagctggtat agttgaccca
     4621 actaaagtta gtagaagtgc tttacaaaat gctgcatcaa tagcaagtac tttcttaact
     4681 actgaagctg ctgttgctga ccttcctgaa aaagaagatg caggaatgcc aggaatgggt
     4741 ggcggaatgc ctggtatgat gtaatttaaa aaatttaaaa tataaatttt aatataaaga
     4801 actatttcga cttagatttt aaactctaag ttgaaatagt tcttttgtat atatctaatt
     4861 tttgtatatc tataaattca atacacatat ttagataatc agtatctagt ttgtaataat
     4921 attttagagt agtattgtta ttttttaggt tatgatttta gatatatatt aattaaggta
     4981 taattgttga tagagaataa atcaaattta aaaggatgcg ataatatgga cttaagaaaa
     5041 ttgcttgaag aagttaaaaa tgaaaatatt gatatagatt ttgcgttaga aaagttaaaa
     5101 gatttacctt atgaagacct tggatatgca aatatagacc atcatagaga attaagaaat
     5161 gggtatccgg aagttattta ttgtgaaggt aagagtgatg agcatatttt agggattatt
     5221 gataaaatga atcaaaaaag ttctaacata ttggggacaa ggtgcagaaa agaaactttt
     5281 ctgaaaatta aagaaatcta taatcatgct gagtatgaag aggtgtctaa aattcttaag
     5341 attcaaaatc atgatataga gaatatagga aaaggtaaaa ttgtgattgc tacaggggga
     5401 acttctgata taccagtagc agacgaagct tatcatacag ctaagttttt aggtaatgat
     5461 gttgatagaa tatatgatgt tggagtggca ggtatacata ggcttttaaa taaaagacat
     5521 aaaatagata gtgctagagt aatagtagcc gttgcaggta tggaaggtgc tcttgcaagt
     5581 gtaattggtg gactggttga tgttccagta atagctgttc caacttctgt tggatatgga
     5641 gcaaattttg gaggattagc agcattacta gctatgttaa atagctgtgc atctggaata
     5701 tctgttgtaa atattgataa tggatttgga gcaggatatt tagcatcaat gataaataaa
     5761 ctgtaaatac attgatataa attaaacaaa actatataat aaaatttaat ttctattata
     5821 tttttactat taaaattgag gagacatata tgagtgaaaa aatactttat tttgatataa
     5881 taagcggtat atcaggggat atgacccttg caagtttatt aaatttaggt gtgcctaagg
     5941 aaattttttt ggaggaaatt cataaactaa gaatgagcga tgaatttaat ataaacatca
     6001 gctcgaaaac tgaaaatgga atagtaggta caaaagtaga agtaattaca aaagaaaaac
     6061 atacgcatag aaatttagtg gatatatttg aggttataga taaaagcact ctaaacgaaa
     6121 atgtaaaatt taaagcaaaa aaaatattta tggtaattgg agaagcagaa gcaaaagttc
     6181 atggaactac aatagacaag atacattttc atgaggtagg ggctatagat tcaattgtag
     6241 atatagtagg agcttctata ttagttgact tactttgtgt agataaagtt tgtgctagta
     6301 cagtgtctgt tggttcagga tttgtaaaat gtgaacatgg aattatacct atacctgcac
     6361 ctgcaactat agaaatttta aaaggtgtgc caattaaatt aaataatgta aatggagaat
     6421 gtacaactcc aacaggagct gcaatcataa aagtcttgtg tgatgagttt gtagatgagc
     6481 ttgaattttc tccaaagcaa attggatatg gtataggtca taagaagttt gaagtaccaa
     6541 atatactcag aactgtatta ggtgaaaaaa aaaagaagaa ttaatttatg aaattagtgc
     6601 aaacattgat gatatgtctt cagaaatcta ttcttatttg tatgaaaaaa tattagatga
     6661 gaaagcatta gatatttata cagagagcat ttatatgaag aagaatagac cagcaacaaa
     6721 agtatcaatt ttatgtaatg aatcagattt agaaaaattt attaaaatat tacttttaga
     6781 aactagtact tttggagtga gatataatgc atataataga gaaattctag agagaaaatt
     6841 tgtaaaatta gatacaaaat atggagtggt tacagtaaaa ctagggtatt ataaaggaaa
     6901 actagtcaag gctactccag aatataatga atgtaaaact atagcaaaaa atgaagatat
     6961 tcctataaaa aaagtgtttg ctgatataaa ttttagaata aaagaagaat ttagtgaaaa
     7021 cttattgaca taagtaataa attttgataa attaatattc agccttatta aataaaagta
     7081 aacagaaaaa aatatataaa atataaactc actcgtatat gctcagaaat atggtctgag
     7141 agtctctacc aagataccgt aaatatcttg actatgagtg aaattattat accaaaagac
     7201 ctagttaagt aaatgtgtag gctgagttaa actccctatg tgttggctat aagataaagg
     7261 tattttggaa gcctgtatag taatttcact tttgctagaa attatggaag ttactataca
     7321 ggttttttgt gtaaaaagat ttaatataat ttagactgta ttgtgttaaa ataaatatat
     7381 aaaaactata ttatacaagg agagatgtaa atttatgaaa catgaattag tacttgtaat
     7441 agattttgga ggacaatata atcagttgat agctagaaga gttagagaaa acaacgtata
     7501 ttgtgaaata cttccatgta ctgcaagcat agaaagaata aaagaaaaaa atcctaaagg
     7561 gataatattt acaggcggtc caaatagtgc gtatttagaa gattctccaa caatatcaaa
     7621 agaaatattt gaactaggag taccaatact tggaatatgc tatggtattc aaataatgtc
     7681 tcatgtattg ggtggagttg taagaaaagg taataaacaa gaaaaagaat atggaaaaac
     7741 agctataact tatggaaaat catcattatt tgaaggaata actacaaata gtgtatggat
     7801 gagtcataca gatttaatag aaaaagttcc agaaggattt actatagttg caaatactaa
     7861 tgattgtcca gtagcagcta tggaaaatgt agaacgaaac ctatatggag ttcaattcca
     7921 tccagaggta gaacactgtt tggaagggga caagatactt acaaacttct tatataatat
     7981 ttgtaaagta aaaggtgact ggactacaga ttcattcata gaagataaaa taaaagaatt
     8041 aaaagaaaaa ataggcgata aaaaagcttt atgtgcttta agtggaggag ttgattcttc
     8101 tgttgctgca gttcttattc ataaagcaat tggggacaac ttaacttgta tatttgttga
     8161 ccatggttta cttagaaaaa atgaaggaaa tgatgtagaa agaatattta gagagaaatt
     8221 tgatataaat ttaataagag ttaatgcaga agatagattc ttatctaaat taaaaggagt
     8281 ttctgaacca gaagctaaga gaaaaataat aggtgaagaa tttataagag tatttgaaga
     8341 agaatcaaat aaattaggaa aaatggattt cttagtacaa ggaacaatct atccagatgt
     8401 tatagaaagt ggacatggaa atgctgctac tataaagtct catcataatg ttggtggaat
     8461 cccagaagat gtagacttcc aagaaattgt agaaccatta agagagttat ttaaggatga
     8521 agttagaaaa ataggattag agcttggtat agaagaagga ttaatattta gacatccttt
     8581 cccaggacca ggtctaggaa taagagttat aggtgatgta actaaagaaa aatgtgatat
     8641 attaagagaa gctgatgctg tttatatgga cgagcttaga aaagcaggtt tatacagaga
     8701 aatatggcaa gcttttgcaa cattaccaga tgttaagact gttggagtta tgggtgatga
     8761 aagaacatat gcttatttag ttggtttaag agcagttact tcttctgatg gaatgacttc
     8821 tgactggtat aagatgcctt atgatgtatt agaaagaatt tctaatagaa taataaatga
     8881 agttgatgga gtaaatagag tagtttatga tataacttct aagcctcctg gaactattga
     8941 atgggaataa aatatagaat gccaaaaatc cacataaata caggatttga ggtctatctt
     9001 acttctatgt tatactggtt tgatactatc aatatttgcc agtatcttat ggattaataa
     9061 gtaaaaaaca tcctgctgaa tgataaattc agcggggtgt ttttattttg ttttagaact
     9121 cctttgctct gaaactcgaa gtatttaaca ctgatgattt tgttatcttt tagttccttt
     9181 tgtgtcatgc tctgaaagac aaattgatac aataaaaaag taatttctta atcattgatg
     9241 gagatatgta tttatgttac tcttgatggt aatttgagta atattgttca tagcttttta
     9301 tattcactta attagaaaac catttggtat aatcataaat aaacttgatg atataataga
     9361 aaagatttat acttattaga agtatataaa agtattttat gtctatgaaa tgtatgtcta
     9421 taaaacatat agatgacttt tgtatattaa tgtatgaata ttgatattat agaagaattg
     9481 agttgtcaat gatactaaat caaatattta tagaagctgg agggattatg agaaataaaa
     9541 taattatagg aattactttt tcacttgtat tactaatgtc tggatgtgct tcaaaacaaa
     9601 ccgcagaaat aaacgtatta gcaactactg atttacatgg agttatacct tatgagttaa
     9661 cttcttatgt aaaagaagaa agaaaaaaag ataaaaatct aactttagta gatgcaggag
     9721 atttttttga tagtggagaa gtgtttggtt ctagcatgga tagttatttt agtgctagat
     9781 ataaagatgg tgataataac actgaaagtt atattgagac gcctatttca aaagatatga
     9841 aagaggtagg ttatgatgtt gcagttcttg ggaatcatga atttatatcc aacaataaat
     9901 tttatctaga taatatgata tctgattttg agaagcaaaa aataaatatt ttatcggcaa
     9961 atacgtataa aaaaaatgga gacagttata caaaaccata cataataaaa aatattaata
    10021 cacctgaagg aaacgtaaaa ctaggaattt tagggcttac tataaaagaa gttggtgaga
    10081 ggaaacagtg gaaagatgga aaacttgttg actctaagtc tttagaacta aaagaccaag
    10141 agggctacaa tggtgaattg tatatgaatg atttagtaga agatgctaaa aagtgggtta
    10201 gtataatgga aaaagagaag cctgacatta tagttgctgt ggcacataca ggtgagaaac
    10261 ctaagaaacc tagaaaccca ggaaatagaa tacaagactt agctcaacaa gttgatggaa
    10321 ttgatgctat agttgcaggt cataatcatg ttcaaattga gcaacatgat tataaaaata
    10381 aatcaggaga aaatgttata gttactgagc caggaaagca tggagaatgt atttctaaga
    10441 ttaattttaa attagaaaaa aacaatgata aatggaatat agttgacaaa tctagtaaat
    10501 taattaaatt tgaaaaaaat caagaaagtg ataaatttgg agaatttatt tcttcaatta
    10561 ataaagtaag taaagataag aaagaaataa gattatcaga agtaaagact cctgaatggg
    10621 ataaaatata tgtttttaaa tctggaacat ctgcagatga tatatataaa acgataggat
    10681 ataaatggag aaatatatca tctccaaagg gtgatgacat ggtgcagatt gttgtaatga
    10741 ataataataa gcctgttagc tatgtatatg gtgaagctga agttatgcca attaatataa
    10801 aatgtgatga atcagagtat aaagataatg ttgtaactat ttatcctaat aaaaatgata
    10861 aatttaaaat tgaaaaaggt aaacaagaat atagtattaa tttaaaacat atacaataga
    10921 aacttttatc ttatttaagt ttagattgta cttaaaataa agtgtgtatt attttaaaaa
    10981 ttgatgataa ttaaaatcac aacattaagg aatagtacac actttttatg ctaaaaaata
    11041 gaatgttaaa aaattaaacc tttaaattaa agtacattgc ttatattatc caatattatt
    11101 ttcaaaggtt gtgtaatatt tttatcttta tgtaatattg ctaacatttt attttccata
    11161 tctatcccat ctaattcaat caatcgcaat tttttatgta agagtgaatc taataataaa
    11221 ttttctggca aaatggttat acctaaccct gcttctgcag ctttaataag tgcaaaagaa
    11281 ttaacacttt cccaaacagg ataagctttt tggtttgaaa gagataatac actgtccaaa
    11341 gtatcgcgaa tggcacttcc ttgctcacgt aacaataacg gaaacgtgca taactgtcgt
    11401 agagataagg cttgtttaga taaaggaaaa gttggagcac atgcaacaca taacttatag
    11461 gaacctaata aaattgtacg gaatgccccc tgtggttcta taccttccca aaagcatata
    11521 tcagcttctc cactttttaa aatatccata gctgaattag cactttcgac acgtacattc
    11581 actttaagag atgggaatga attttttagt cgacttaaaa ttggtggaag taaaaaagag
    11641 gcaattgtaa tgctagaaac aatgtttata ggtgtacatt cctctaaatg actgattctt
    11701 ttgtctaaat tacgacaagc agttaagata ctacgtgctt cttctaaaag agaaatgcca
    11761 cagcgagtaa gacatacccc tcgtggtagt ctgtcaaata atgctgtttc agcttgttgt
    11821 tcaagttcag ctatagcatg agatacagca gattgtgtga tatgtaattt ttttgcagca
    11881 cctgtaaaag tgcctgtttg ttcaattgct tctaaaatct ctaaatatcg tatgtttatc
    11941 ataataaata tcctcactag acatgaataa aattgattaa ttccattaaa ttctatcatt
    12001 ttacagattg caaagcaaca ggtatactga ataatatgaa gtgaaaagaa ggaggaatat
    12061 tttgaatcaa agcagaaaaa catatttttg gcttttaatt attaacttgt ttattagcac
    12121 attcactttt ggtggagggt atgtagttgt acctatgatt agacgttatt ttgtaaatca
    12181 aaagcaattc tttacagaag atgatttgat gagcatggca gcagttgcac aatcaactcc
    12241 tggagctatt gcaattaatt tatctgcttt ggcaggttat cgagtagctg gtactttagg
    12301 ggttgtcatt agttgtatag cagctataat acctccgctc gttattcttg gattagtatc
    12361 agcattttac accactttta tatctaatgc cattgtaatt gcagtattaa aaggaatgca
    12421 agctggtgtt gctgcactaa ttgtggattt gattattgac atgtgctcta tgatattaaa
    12481 acaacgttct ctatttttat cagctatgat acctattgct ttctttgcaa actttgtgat
    12541 gagtataaat gtaattttta tattatctgt ttgctgtttt gtatgtgtaa tgcaagtttt
    12601 ctacaagaag agggaaaata aatgaataca atttggaatt tattttttat atttttaaaa
    12661 ataggcttgt ttagtattgg tggtggttat gcaatcatac ctttaatcca agagaaagta
    12721 gtaaatcaaa ctggatggat aagtgagaaa atgtttacag atattataac tatttcacaa
    12781 atgacaccag ggccactggc tgttaatact tctacttttg ttggacttca aattggaggg
    12841 ttttggggag cagttgcagc cactttaggt tgtgtattat gtggtgtagt aatatctcta
    12901 tttttatata atttttttca aaagcatcag aactctactt atatatttga aatattaaat
    12961 ggtctaaaat cagcttcatt aggtttaatt atctcagctg cggctacaat tataatcctt
    13021 gcattatttg ggacgaataa agtgtacttc aattctatat ttgagatgct aaactggact
    13081 gcactaataa tcttttttgt tacactactg atagtacgta agtggaaaat taatcctatt
    13141 gtgattataa taataagtgg tatactggga atattttttt atttataagt ttttgctaag
    13201 taaaggatat ctattttttt atatcttaaa tccaaatagc caataaagta aggctatttg
    13261 gatttttaat aaattctgaa aagataatca gaaacccata taacatttaa attatatcac
    13321 tggttttcta caaaccaatc ataagcttct tggcaaaatt tctcactatc tatatagtaa
    13381 ctattatgaa cattattaat ttcaacaagt ttctttttac attttagtga attataatag
    13441 ctatcaaaat attgggtttt aaaatatcta tcatgaagtg ttttaaagac aagagtaggt
    13501 atgttgactc catcttttat cttactatct tctgcaataa agcattctaa cattgtatta
    13561 attatctttg tactattttt ccaaatatga ggatttttct tttcaaatat tttataaaat
    13621 tcattatcat agacttcatt ataaccttta tatttaggtg gttttactgt tatgtgaaaa
    13681 ttaggaagaa acttaaagaa aaaatataat aatttttatg ttctgtttta tatattattc
    13741 atattttatc acagataata tactaaagtt aattttttta ttgtacaact attataataa
    13801 ttcttctata atataagaca ttctataatt ttatatttaa gttcatgtgt atttaattgt
    13861 ttttatataa aatgtccagt aatactattt ggatttattt ttatttcatc aggagttcca
    13921 gaagcaacaa tttcccctcc atttattcca ctgtctggac ctatatcgat aatccaatca
    13981 gaagcacgta taacctctgt attgtgttca ataattaaaa gtgaatgctg ttcatttaca
    14041 agttttctaa aagtaattaa caaattttct atatctttaa aatgaagtcc agtagtaggc
    14101 tcatccaata tgtacagcat tcgtttttta gtttttgtcc ctaattcttt tgctaatttt
    14161 atcctttgag attctcctcc acttaatcct aatgtatttt gtccaagctt tagatatcca
    14221 agtcccactt catctaataa ggagagtttt ctatatatat cagagcaatc gctaaaaaat
    14281 gtttttgctt ctgctacttc catgtcaagt atatctgcaa ttgtctttcc cttgtattgt
    14341 atagacagta tttctttttg atatctctta ccttgacatt tactacaagt tacccattgg
    14401 tctgccatat attgcagaat aatttttgtt ttacctaatc cttgacagtt tgggcattgc
    14461 ccctttttgc tattaaagct aaaatagctt tcatccaata atttttgttt tgcttgtgga
    14521 gtttcagcaa atagagaacg aatttcatca aaaatattga tatatgtagc aggtgtagaa
    14581 cggctagatt ttccaatagg tgtttggtcc attaagataa aatcatcaat agcatcaaat
    14641 ccagtaaatt cagtataatt tttatctggg atagattttt gtttcaattt ttcttctaga
    14701 gcaggaagta aactgtgaaa tacaagagag ctcttaccag aaccactaac acctgtaata
    14761 gagcacatac agtttaaggg aatttctaca tcaatatttt ttaagttatt tgcatggcat
    14821 ccttttaagc ttaaccattt acttgggata attttatttt gtgaacctgt tttattgtat
    14881 gtacttaaat atttacctgt tatagagttt ggatttttta taatttcttg ggttgtacct
    14941 tctgcaataa tgaacccccc ttttgtacca gcacttgggc ctacatcaat aatataatca
    15001 gcagacagta ttgtatcttt atcgtgttcc acaactataa cagtattacc cttatctctt
    15061 agctcttcta tcattttgat gattaaatta tggtctcttg ggtgtaaacc aatagatggt
    15121 tcatctaaaa tgtaggttaa gcctactaat tcacttccta gctgacttga taatcgtact
    15181 ctctgcagtt ctcctccaga taaagttgga gctgttctat ctgttgatat atagctaaga
    15241 cctaccttta atagataact aactcgaatt tttaattctg ttaagatatc atttaccaaa
    15301 gagagctcat ttttttgaag ttgatttggt aattcacaca accaattcca taactgttct
    15361 atcgtcattt ttgttagttc agggaagcga taacctttga ttgtagtgaa tctagcctca
    15421 atgcacagca attcgccacc acatgtattg caaggaattt tgttcatata ttgatgcaag
    15481 gtattatttt cagaactaga ttctctaaaa agtctttgaa tgtggttgat tgctcctgaa
    15541 gcaggtcttc taatctttga ttctcgtccc tttgattcaa agctaaattc atatatctta
    15601 tcgtctgtac catataagat tgcatctata aaatctcttg gtaattcatt ccaaggtata
    15661 tctaggtcta tatttttatc ttttgctatt gcatataatt cacctaccat ccaattacca
    15721 tttggctttt tccccctcaa ttttccaaaa tagtaagtgg ctccatctaa tattgaaata
    15781 gttgggtctt ttacaatcaa ttgaggatta acttgatagg taaaacctaa tccattacaa
    15841 tcgtgacaag ctccagtatg ggagttagca ttgaaggttg aagtatttat cttagaaaaa
    15901 atagtttgac aatgtggaca ataatttttt gtattattct ttttttctaa tggaattttg
    15961 caatatggac agtaactttt tccgattgat acaaataaca ttcttaaata atcccaaatg
    16021 cttgtaagag taccaacagt agagcgagga ttttggcttc cttttttctg ttcgatagct
    16081 atagtagggg ttaatccctg aagtaaatca aattccggtt tttgaagctg ttctgaaagt
    16141 tttgctccat tacttagatt ttcaaaataa cgtctttttc cttcttcata aatcgtatcg
    16201 aaagctaaag aagattttcc actaccactt acacctgtaa taacagttat ttgatttttt
    16261 ggaattgtaa cctcaacatc ctttaaattg ttttgccttc ctccttttaa atagatagag
    16321 cttttattat gacaaagtga atttttaatt gatttattta ttgattttgt aaatttgaat
    16381 ttcttttcga gttcaagagg attgcctaat tcttttattt ccgaaatgcc taatattttt
    16441 aactgagaaa agattgattt caaataatct tcaggcagtt tttctattaa caaattaagg
    16501 tcttctattt ttatatttcc gattgataat atttctgcca tttcatctaa taaatttttt
    16561 tctgctcttg ttaattgatt gtattttttg gaacttaatt ctgtccataa atcccatgga
    16621 agcaattcta aaccaatcat attattaaag tcatgtagaa gattaccctt tacagaatgc
    16681 caacctttcc aatttttatt atatccaaag tcatctggat gagcatgtcc ttgccgacat
    16741 cttatccagg cttctgaacc agttaaaaaa ccatctgttt ttccgacatc atgagtgtca
    16801 aaattaattt gaaaataatt tttttgtatg tcatctattt gtgcatctat tcttatccat
    16861 ctctttgtta gagtatcata gtattctaca agccagtggt cttcataagt aagctcactt
    16921 tcaaaataat tagcaaaacc tgctctcatt ctagcctcaa aacctcgata tcttaggagc
    16981 gatacaagaa gtaaagaata atctctgcac attccaacaa gcctttgttt tgggtttctt
    17041 ggaatagtta gtgggttaga atttatttca aatattcttt ttaacatttg aggaattgta
    17101 cgaagcaatt cctcatcaga ttgttgttta ttaaaggata taccatataa ttttccttgg
    17161 tctccatgaa ttactaggct ttgaaccact tttaataact caattggagt ctttggtaaa
    17221 tcttgatatt tattttcaaa ttttttagga tttgtgatga tactttgatt tttatagtta
    17281 tttattttca tttatatttc acctctctaa atggatgata taccctgaca tggtgtcata
    17341 tgcaaggaca atttagattt tttggccaaa atttaaaaat aaataaaaaa tgatacagtg
    17401 ataaattgta atacaaatat agtttcaaat tgcatataat ataattattt aataaaacca
    17461 ttatctattg aatgttaatg aaatgttaag atggcattct tgaatattaa gaaatagaat
    17521 actataatat agtgtgaagg atttttagta aaaaaatttg taagtgttaa tatacattaa
    17581 aaaataggtt tataatttgg aggaaaaaaa tgaaattatc aaggtataag aaaaatagtt
    17641 tagttctgtc tataatttta tgttcactta ttatttcaat ccttgtaatt actgttttta
    17701 ataccgtaaa actacacaat atatctaata aacaatctaa aaactattta aatgatgtat
    17761 ctacacagat tgtaatgaat gttgattcta aaatcaaatt tattttatcc gacttaagaa
    17821 taatggcaga ttttattaag caatatgaag gtgatagtcg atatgagtat ttatctaaaa
    17881 gaaagactag ttataagtat tatgatgttg gaataataga tttagaaggt aaagctgagt
    17941 ttttaagtgg aaaaaagttt gacttaaaga atactgtttc ctataaaaaa ggaattgaag
    18001 gtaaagaata ctcagaagta atagagtcaa tgggttttgt tctttatagt gtacctatat
    18061 ttaatgataa tcatgaagta tctagtattt taataggtgt atccaataaa gaaagtatga
    18121 atgagattct taatatagat aactttaatg gaaaaggaac tattgaaatt attaatagta
    18181 agggaaaacc tcttttcata ggaaaaaata ctgaattaat aaaggacctt agccataaat
    18241 ataagagttc tggagatgaa ccttgggcac aaaagatgct tgaggatttt aaaaatgata
    18301 aatcagggaa tattacaata acctcatcaa aaggtgtaca atgcttatta acttatcatc
    18361 caattacaag aggtttaaat gattggcatt ttcttctcat agtacctgaa gatgctgtac
    18421 ttggtgaact taataaatta aactctttta caataacaat gacatttata attacatgta
    18481 ttattggaat tataacatta attttatata ttataagaag aaaatatgta aatcaaatag
    18541 aaaatattgc atatttagat agtataacaa atggtataaa ttcaactaag tttagtatgc
    18601 tagtcaaacc tttaatttca gaatcacctg atagtactta tatgatgata gcaatgaata
    18661 taaaagattt taagctaata aatgattgtt tcggtagtga aaagggaaat atgacactaa
    18721 aacatcttta caatattttg tataaaaaca ttgatgaaaa tgaagagttt gtgtgccgtc
    18781 atgatgcaga ccttttctat ttgcttatta agaatagacc agtgttagaa gccctggagt
    18841 tattatataa aatagaagct gacacaaatt attttaataa aaatagggaa aatccatact
    18901 ttttaagact atcagtaggt atttacacaa ttgaaaatca taatgaagat ttaattacaa
    18961 tacaagacca tgcaaataca gcaagaaaaa gctttaataa aactcatcaa agtgattttt
    19021 ctttttatag tgatatagaa agaaaacgat taatatctga aaaggaaatt tcaaatttaa
    19081 tggaaaaagc ccttgaaaat aaagaatttt ttatgtgctt acaacctaaa attgatataa
    19141 aaagtggaaa aattagtgga gcagagtctc ttgtgcgttg gaaaaattca gaaaagggca
    19201 taatttatcc aagtgatttt attcctcttt tcgaaaaaag tggatttata tgtaagttag
    19261 atttatatat actagaagaa acatgcaaat tgatttctaa gtggataaaa gaaggtaaag
    19321 aagtgttgag aatttctgtt aatgtatcac gtcaacatct gaatgataaa ttgtttttag
    19381 aaaaatataa gcatatatgc aataaataca atgtaccaac atgtctgata gatttagaac
    19441 ttactgaatc tattttctta gaaaatctag aagctataga tataatagag gatattcatt
    19501 ctaatggatt taaatgttct atagacgatt ttggatttgg gtactcatca ttagggatat
    19561 taaaggattt taaagttgat ataattaagt tagaccgttc attctttgtt agcaaaaata
    19621 atatagaccg tggtaaggta gttattaagt ctattattga acttagcaaa aggctaggta
    19681 tgaaagtaac tgctgaagga attgaagaat tagaacaagt tgagttccta aaaaatgtgg
    19741 gatgtgatta tattcaaggt tatgtttttt caaaaccatt attaatttct gactttgaaa
    19801 attttgcata tgaaaataat aaaattaaaa atatccatta ttaaatgtac aagataaaaa
    19861 atttaggcaa gttatataat taaaaatata acttgccttt tttatttata tatttaataa
    19921 aaatatattt gcagattttg taaaaataat tttcaaatgt tgtaagttgt ggctaaagct
    19981 ttattgtatt attctgaata tagaaagata gttactgtct tagataaaaa ctgaaacata
    20041 cataataata taaaatttgc tatattaata actctaatta ttttggaagg agggaaaata
    20101 tgataagtgg gagaatgtta ctcatcatga attttctaaa aggaaaaaat aagacaagtt
    20161 acaaggatat tagcaaacaa cttagtatag aagaaagaaa agttagatat gatataaata
    20221 atttaaatat tgttttgcgt tcattaaaca agtcagtaat acaaaagatg aacaaaggtg
    20281 tgttaattat tcctgatgac ttccaagtag tatgtataaa taataacgaa tatgtatttt
    20341 ccttaactga gcgagtatct attatgaaaa ttatatcaat gtttaaaata gataatctaa
    20401 acttagaaaa attaagtaaa gaatttcagg tttctagaag ttcaattaaa aatgacctta
    20461 atgttttgtc aagtgaatta gaaagaaacc aaataactat tacttatgat aaatctttta
    20521 aaataagtgg agaagaagac attatatata agcaaagatt gaacatatta aaatcgtatt
    20581 cttatctttt tggtaaggaa aaatctgatt taagtgtatt tgagaagtat ttagtgaatg
    20641 taattgaaaa aatattcaaa gggatttatt tgattgatat atataattgg tctatgaatt
    20701 tattattgca aatgggatgg actttaaatg atgaatcttt tacatggtat gtgtcgaaca
    20761 ttttcctttt tacatggtat atacattcag atgaaaaaca tcctttagaa tctgcttcat
    20821 taagtgagcc gtgttttgaa aataagtttg accaagaatt tgaaacaatt attcaaaaaa
    20881 agttgactaa ggaacaaatt tttctgttaa agagttttgt attttttaca aataaatatg
    20941 caagtttaaa cgaggatatg gatttgatta gtacagaaat tatagttcaa gacctaattt
    21001 acaatatgtc agaaatatta gagattagct ttaaagaaga tatgattctt tataaaggat
    21061 tactcaatca tattgctcca ttgatagaga gaatcaagca gaatatacag atttatgagg
    21121 aattacttca tgtaatacca gataaatatc aatatgtact tgaagctact aaggtttcta
    21181 ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa tgaaacaata cttttggcaa
    21241 tacatttttt aggaagtata catagaaatg agaggaataa ctacaaaaat atattgttag
    21301 tttgtggatt tggatatggt gtaattgcta tgttaaagga tagtttagta aatgattacc
    21361 agataaatat agtagagagt attccatcat ataagttaca agattataaa gaatgggaaa
    21421 gtattgactt cattataaca acatcaaaga tagtaataga cttgcctaaa acaattattg
    21481 agataaatcc atttttacaa gaagaggatt atttgaagtt tgaagaaaaa gggataaaaa
    21541 gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag gttggacttt ttagaagaag
    21601 atgcccaaaa aagagtatta agtattatat gtcaagaact tggatacagt gatgagagaa
    21661 tattatttag acaacttaat ttaagtgatt taatagggat tgataccata aggataattg
    21721 atggagatat gaaatgggaa gatgctgtaa aatttagttc aaacatactt gctaattgtg
    21781 attttgtatc agcacaatat gctgagaata ttatagatat tttaaaaaat gtgggttttt
    21841 atgctgtaaa agataacgaa tttgctttac tacatggcaa tgatagttca ttagtcaaag
    21901 taagttcgat aagtttatta atatgtaaag aagcagttag ttttggagat aaaaaggtta
    21961 aaatcatatt ttgcctagca agcagagata aaaaagagca aattcctgca ataattaatc
    22021 taactaaaat ggtttataag actaatttta ttgctatgtt agaaagtgca aagacaaaag
    22081 aagaagcaga agttttaatt cataaatatg agaaggaggt cactgtatga ataaattagt
    22141 taatgtaaaa tgttcattta aaatacatgt taattcaaaa gaagaagcca ttatatcgtt
    22201 agttgatgta gtgtcaaaag aaggatattt aatagataaa aatcaatttt taaaagatgt
    22261 tttaaagaga gaagaaacat tgtcgactta tattggacat ggtataggtc ttcctcatag
    22321 tcaaagtgta ggtgttaaaa actcttgtat tacaatagga aagcttgata ctcctattga
    22381 atggacagaa gaaggagaaa aagtggattt aatatttttg atatcagtaa caaaagacaa
    22441 tgaaaataat cttcatttaa agattttatc aaaattagca cgtttattga tgcatgaaag
    22501 ttttagaaat caaattagat gttcagatga acaaactgtt tataatctta taaaagaaaa
    22561 aatagaggag gaagattaaa atgagaattt taaagttttt gcaaaaacat cttatgacag
    22621 caacatctta tatgattcca tttgtggttg caggaggaat attatttgct ttatcagtaa
    22681 ctttaagtgg acaagctgct gttcctgaaa caggctggtt ggctaaactg aatcaaattg
    22741 gagctgctgg cttggcttta ttcattccta tattaggtgg atatatagca tttagtatgg
    22801 cagataaacc aggattagct ccaggtatga taggtgcata tttagcaaaa gaagtaaatg
    22861 caggttttat aggtggtatc atagcaggat ttatagcagg atttgttgtt ttacaattaa
    22921 agaaaattaa attagctcct acaatgagaa ctttaggttc tatatttatt tatcctttag
    22981 ttggtacatt gattacaggt ggtataattg tatttttaat tggagaacct atagcttctt
    23041 ttatgacatg gatgacaaac tggttaaatg gaatgtctgg tgtttctaaa attcctttag
    23101 gtggaatctt aggaggaatg atagcctcag atatgggtgg ccctataaat aaggttgctg
    23161 ctacttttgc ccaaactcaa gttgacacac ttccatattt aatgggtgga gttggcgttg
    23221 caatttgtat acctccaatt ggtttaggat tagctacatt attgtttcca aaaaaattta
    23281 gcaaggaaga aagagattca ggtaaagctt cactattaat gggatgtgtt ggaattacag
    23341 aaggagctat tccatttgca acatcagatc cagttagagt aataccttgt attatggctg
    23401 ggtcgattgt aggaaatatt atggcttttt tattaggatg tttaaatcat gcaccctggg
    23461 gaggtctgat agtgttacct gtagttgaca ataggttggg gtatattgca tcagtattaa
    23521 ctggagcagt agttgttgcg gtattaatga aattagtaaa aaaagacgtg aaagaagatg
    23581 aagaaattga agaagattta gatgaatcaa ttgaattaat atttgaagaa ttataaatat
    23641 aaatggaagg aatttgtata aaaaagtaaa tataaaacta taaatttaaa ggagataaga
    23701 atatgaaaat tgtagcagta acatcttgtc ctagtggagt agcacatact tacatgtcgg
    23761 ctgaatcact tgaattatca gccaaaaaat ttggtataga aataaaggtt gagactcaag
    23821 gaagctctgg aatagataat gaattgtcac ttaaagatat tgatgaagca acttgtgtaa
    23881 tattgactaa tgatgttgag attagaaata tggaacgttt taaaggaaag aaggtagtaa
    23941 gaatgagtgt atctgagata attaagaaat ctgatgcatt aattaagaaa ataaaagata
    24001 cttttcaata aaaattgatt tattttacat ataactcaaa gatgttttta aagaaatttt
    24061 tattaaaagc atctttgaaa atatcttaaa aatagaataa aaggagatat agtcatgaaa
    24121 aagttaccaa taaagactgt cgtgcaaaga ttactaaact tacaagaaga ggggaaaagt
    24181 gcaactcttt taggaattgg tcctatgtca ccaaacttgt tacaagcaag ttttgaacta
    24241 gcaaaagatg atgattttcc tttaatgttt atcgcaagta gaaatcaagt agatgcagat
    24301 gaactaggtg gcggttatgt aaatggttgg aatcaggaaa cttttacaaa agatattaag
    24361 aaggttgccg acaaagttgg atttgatggt ttatattatt tatgtagaga ccatggtggg
    24421 ccatggcaaa gagataaaga gagaaatgac catttgcctg ttgatgaagc aatggagtta
    24481 ggaaaaaaat cttatttagc ggacattgaa gcaggttttg acctattgat gattgaccct
    24541 acaaaagacc catttgaaat tggaaaagta attccactag atgtagtact agaaagaact
    24601 gtagaattaa ttgagtattg tgaaaacgag agaaaaagac ttaatctacc agacataggt
    24661 tatgaggttg gtacagagga aaccaatgga ggtctgactt caactgagac gtatgaaaca
    24721 tttataacta gactaaaggt tgaactcgat aatagaggtt tgccaatgcc aacatttata
    24781 gttggtcaaa ctggtacttt aactagaaag acagaacaag taggaacttt taattttaga
    24841 aatgcttatg atttagctca aatggctaag aaatatggag ttggtttaaa agagcataat
    24901 ggcgattact tagatgatgt aactttatta gaacatatac catctcaaat tattgctaca
    24961 aatgtagctc ctcaatatgg tactgaagaa actagggcat atttaaaatt agcagaagtt
    25021 gagtgtaaat tagaaaaaga gggtttagtt gagaaaactt ctaatatcag acatgttctt
    25081 cttgttaatg caattgaatg tggacgttgg agaaaatggg tagtaggaga acaaaaaaat
    25141 ctaactactg aagagatatt taaggatgaa gttttatcta atgaaatact agatattgca
    25201 ggacattata catttaataa tgatgatgtt aaaaaagaaa tagaagtgct ttatgataat
    25261 ttaagtaaga ataatattga tggtcaaaga tttgttgtag accatattaa acgtcctttg
    25321 cgtgattatg cagaatgcta taacttaaaa ggtgttacta cgaggatttt aaataaataa
    25381 taggacttat agtggtgagt attgttatac taggtattta tagttataat tatacttacc
    25441 actattaaat ttaggaggac taatattttg attaatttaa tttttgatgt agatgatact
    25501 ttatataatc aattaacgcc tttttacact gcatataata aagtgttttc ttcaataaag
    25561 gatatttcta ttgaagattt atatatgagt agtagaaagt atagtgatga ggtatttcat
    25621 atgactgaaa atggagaaat gccaatcaaa gaaatgcata tttataggat aatgaaagct
    25681 tttgaggagt tgggaaattc tattacagaa aaagatgcac aaagttttca agatgagtat
    25741 atatatcaac aatcacaaat tacactaata ccagaagttg agaggatttt aaatttttct
    25801 aaagaaagaa atattaatct aggaattata actaatggcc catcaaacca tcaaagaatg
    25861 aagttaaagc agttaaatat tgaaaactgg gttgataaaa gtaatatttt tatttctagt
    25921 gaggttggat tttctaagcc agatactaat atttttagag tagctgagaa tgttatgaat
    25981 ttagatagag aaaatacata ttatgtgggt gattcttata gaaatgatgt attaggtgct
    26041 aaaaaagctg gttggaagag tatatggttg aaccatagag gacatgaggt agaagagttg
    26101 ttttataaac ctgattttgt aattttggaa cataaggatt tgatatcttt atttataaaa
    26161 atatgtagtt ataaaaatat gtagtaaaaa aatgttaata atagaattgt ataaaataat
    26221 taataataac tatataaaat tatatgttta gattgtgagc aatctatttt atgttaaaaa
    26281 tagattattt agaagaaggg gaaatcttaa aaaaatttga atatcccctt tttattattt
    26341 aattaaaatg aaatagttag aaatatattt tatttagttt agtcaataac attttgggat
    26401 tctttataac tacatgttaa ctgttgaata ggtttatcat atggtttaac ccatccttta
    26461 ttaatactta attctgtaag tgctgtgtga ccttctacca tttgagttaa tgaagctgaa
    26521 tatatagctc ttaattcagg tgtactactt gtcaatgcag aattaagata catattggca
    26581 gatgctttag cagatgaaag catacttaac attattactt tatcgtctat ataagtattg
    26641 ttcttaatta gattttctat catatttctc atgttatcta tacctcctca gaaattagta
    26701 tttcattttc aactataaat tgttgtatag ctttaattct tcattctgtt gctaatatac
    26761 tagattctga ttgtcttttt aggtcttcat ctgatattaa catatttatt gctctttgca
    26821 taagtaatcc gtcagactcc atttttataa ttgctgctaa agatattttt tcagtttctg
    26881 aaagtattct catttaatca tctcctatat tgtaatttat gctttaatag tatgtctttc
    26941 ttttcaagga aataaccatt acactttttt acagtatttt atattctctt tatatcaaaa
    27001 tttagtcgac ctaattaata taattttgta ggatatttat cattaaatat ctataaaata
    27061 aattatttga atattcataa agcaaaattt atttgtatgt acttcttcaa gagtcaatat
    27121 gagttttttc aaaaaattaa tttttatgat aatcattaag ggctatattt ttcttaaagt
    27181 tgtgattatc attattaatt taccttagag gttaattagg tgtttaatca aagttaatgc
    27241 ctttagaata caaatgtatt ctaaaggcat ttttactatg tattttcatt tatattacta
    27301 aacattatct atttttatat acatttctat gtattttatt ttcatcaaat cggtctataa
    27361 actcattttt ttcttcacta tatccaatag atataagtgc ataaggaact aattttaatg
    27421 gtaagtctaa agtttgtcta actttattta ccactttatt tttaggaaat actcctgccc
    27481 aacaagcagc tagaccttga ttagtaactt ctaataataa attttgtgtg caagcaccta
    27541 aatcttgtat ccatttacca ggttttaaga atttatctct atttccaagc actactatac
    27601 ataatggtgc aaattctata tgctttgctc tatgctgtga ctttgagagc tgtttcagca
    27661 gttccttatc atctactata ataaattccc aaggttgagc attttttgat gaaggtgctt
    27721 gcatagctga ttttagtatt tcagttattt tatcttcttc tatattttta tctatatatg
    27781 ttcttattga acgtctattt tttataactt ctaacataca gattctcctt taaataaaat
    27841 tatttctatt tatataatat cattacttta aattttagct attacttata taaagaataa
    27901 actttgtgtt ggttttatct aagtaaaagt aacattcaga actaaaaaac aagactataa
    27961 atccaaagta ttaattaaaa aagaagtaat aaaaaataat atacaagatt taaaaaagag
    28021 tattgggctt aaaatttaat tagaaaacca attttgagat aatctgtttt ttattttaac
    28081 aaaaaaacat aaataaagtt cgatacgtag cgaatgaaaa aattacaaga aatatttttt
    28141 ttaaaaaatt tcaaaaaaga gttgataata tatgtatatt aatataaaat ttaaggtgta
    28201 taattcgtat aataatacga atgatttgta gtgtaaaata acttataaac ggagagggga
    28261 agtctaatga aagtagcagt agttatgggt tcaaaatcag attatccaaa attagaagaa
    28321 gggataaaac tgcttgaaaa atatggaata gaagttgtag caagagcatt atcagctcat
    28381 agaacaccag aacaactttc aatattccta aaagaaatag aagatgatac tgatgtaata
    28441 atagcagcag caggaaaagc agcacactta ccaggtgtaa tagcttcaca aacattaatt
    28501 cctgtaatag gattacctat taaatcatca actatggatg gactagactc acttttatca
    28561 atagttcaaa tgccaaaagg aatcccagtt gcaacagtta caatagactt aggtttaaac
    28621 gcagctttat tggcattaca aataatgact ttaaaatacc caaaattaaa agaggatttg
    28681 aaatcatata gagaagagat ggcacaaaag gtgttagaag acgacaagaa tttaaggggg
    28741 tagcagaaat gttattatat gaaggaaaag caaaacaagt atattctaca gataatgaaa
    28801 atgaatatgt tgtttattat aaagatgatg caacagcatt taatggagaa aaaaaagcag
    28861 aaatatcttc aaaaggtata ctaaataata agatatcaac aataatcttt gaaatgttaa
    28921 aagaaaataa tataaatact cactttataa aaagtttatc agatagagaa atgttagtta
    28981 aaaaagtaga gattttacca ttagaagtaa tagtaagaaa tattgctgct ggttctatct
    29041 gtaaaagagt tggtcttgaa gaaggtgtag ttttcgatga gccaattttt gaaataagct
    29101 acaaaaatga tgcttatggg gacccaatgc taaatgatga ttatgcagtg gcaatgaagt
    29161 tagcaactag agaagagtta aaattcttaa gagaagaaac tttaaaaata aatgagttac
    29221 taaaagcatt tttcttaaaa ttaaacctaa agttagttga ctttaaaata gaatttggta
    29281 aggattctga aggaaatata atattggcag atgaagtatc accagacact tgtagacttt
    29341 gggatgtaaa tacaaatgaa aagttagata aggacagatt tagaaaagac cttggagacc
    29401 ttgtagaagg ttatacagaa gtactttcaa gaatgaataa taaataggag gcaaatgtat
    29461 gtgtggagtt ttagggattt actctaataa agatgtaact aaagaactat attattcttt
    29521 atattctatg caacacaggg ggcaagaaag ctgtggttta gctcttttag atgatggaga
    29581 aattaagtac aaaaaagata tgggattagt tggagatgtt tttaaggaaa atgaattatc
    29641 taagttaaaa ggaaatatag ggattggaca tgttagatat tcaacagctg gaggaagtca
    29701 tgtttctaat tgtcaacctt tagttggaag ttgtagaaaa agacaattag ccatagctca
    29761 taatggaaat ctagttaatg caaattattt aaaagatatg ctagaagaag atggatatat
    29821 gttccaaact aattcagata cagaagtgat tttatatata cttgctagat attacaaagg
    29881 ggatatagta gaaagtttaa aagtgacaat ggattatata aaaggtgcat atgctcttgt
    29941 aataatgagt caagaagaat tggttgcagt tagagaccca catggattca gacctcttgt
    30001 acttggtaaa aagggtgatg aatatatatt tgcatcagaa aattgtgcaa tagatatact
    30061 tggcggagaa gttataagag atgtagagcc aggagagata atagtagtca aagatggaga
    30121 actaaaatca tatttttatt cagaaaatta taaaccagtt aagaaaagtt gtatatttga
    30181 acacatatac tttgcaagaa atgatgcaac tatagacaat gtaaatgcat atgaatttag
    30241 aattaaatgt ggtgagagat tagctcagaa tgaaactgta aaggcagata tggtagttcc
    30301 agttccagat tcaggttggc caggagctat tggttatgca aatgctagtg gattaaaaat
    30361 aagtgaaggc ttagttaaaa atagatatgt aggaagaaca tttataaaac ctacacaaga
    30421 agaaagagaa atagcagtaa aaataaaatt aaatcctctt tcaacaataa taaaaggaaa
    30481 atctataatt ttagtagacg actcaattgt aagaggaact acatctaaac aattagtaaa
    30541 atctttaaga gaagcaggag caaaagaaat tcatcttaga ataacttctc ctccagtagc
    30601 ttactcttgt tactatggaa tagatactcc aaatcgctct aaattaattg catcaagtaa
    30661 caacgtagaa gaaatgagag aatatattgg atgtgatagt ttaaaattcc tagatataga
    30721 aggaatgtta gatgcaacag aacataagtc tactttctgt aaagcttgtt ttgatggaga
    30781 atacccagtt aagaaaattg ataaggagga actactttca tgttaacata taaagaatca
    30841 ggtgtagata tagatgaagg aaatagagca gtagacctta taaaaggtaa aataaaagga
    30901 acttatgatg gaaatgtagt tggagattta ggtaacttca gtggattata cagtttaaaa
    30961 gattttgtag gtatgaaaga accagttttg cttgcatcta cagatggtgt tgggactaag
    31021 ttaaaaatag ctcagatgat ggacaagcat gatactgttg gaatagattt agttgcaatg
    31081 tgcgtgaatg atttaatttg tcaaggagca aaaccattat tcttccttga ctatatagct
    31141 cttgggaaat tagtgcctga gcatatagaa aaaatagttg gtggaattgc agatggttgc
    31201 aaaatgtctg gttgtgcact aattggtgga gaaactgctg aaatgcctgg aatgtatgga
    31261 gaagatgact atgatttagc aggattttct gtgggaatag cagacaaaga aaagattgtt
    31321 tctggaaaca atgttaaatc aggagatgta ttggttggta tatcttcaag tggagtacac
    31381 agcaatggat tttcttttat aagaaagata tttttagaaa cttacaatta taaaatggaa
    31441 caatatgttg aagaactagg aatgacagtt ggagaagcac ttttaacacc aactaaaata
    31501 tatgttaaat tagctttaga tgtattagct aaacatgaca taaaagctat agctcacata
    31561 actggtggag ggcttataga aaatataaca agagttatac ctaaagggtt aggactagat
    31621 atcaataaaa aatcttggga gaagcctcct atatttaaaa tgatagaagg ttttaatgct
    31681 gttgatgaga gagaacttca taagagtttt aacatgggta ttgggctagt tttaatagta
    31741 gataaagaaa atgctgatga tgttgtaaac tttataaata atagagaaaa tgacaatgct
    31801 gattatgtag ataaaaaata tagtgaatta ttagaagata aagcttatat aattggtgaa
    31861 gtagtagata gtcatgaagg tgtagaatta tgctaaatat aggagtttta atatctggtg
    31921 ggggaacaaa tttacaagca gttatagatg gtactgagtc tggagaaata aaaggtcagg
    31981 ttaaagtagt tatctcaagt aagcaaggtg cttatgggct agagagagct aaaaatcata
    32041 acataaaagc aatttgtgaa actgatgaag acaagataat agaaattctt aaggaaaata
    32101 aaatagattt agtagttttg gctggttatt taaaaataat cagcccaaag ctggttaatg
    32161 agtttagaaa caaaatgata aacatacatc catcattaat tccttcattt tgtggagctg
    32221 gattctatgg agaaaaagtt catcaaggtg ttatagatta cggagctaaa gtaactggtg
    32281 ctacagttca ttttgttgat gaaggtgctg atactggacc aatcataatg caagatgtag
    32341 ttaaggtaaa tcaagatgat gatgcaaaga cacttgcaaa aagagtgtta gaagttgaac
    32401 ataggatttt aaaagaaagt ataagtcttt tttgtgaaaa taaaataaaa cttcaaggta
    32461 ggagagtgtt tataaatgag taagagagct ttaataagtg taactgacaa gactggtgta
    32521 gtggaatttg ctaaagagtt aaataaatta gattatgaaa ttatatctac aggaaataca
    32581 tttaaaacat taaaagaaaa tggtgttaat gtaatgcaag ttgaagatgt tacaaatttt
    32641 ccagaaatat tagatggaag agtaaaaaca ttaaatccat atatacatgg tggtatactt
    32701 tataaaagag ataaagaatc tcatgtagaa actgtaaatg aacacaaaat acattcaata
    32761 gatttagtag ctgtaaatct atatgatttt gaaggaactt taaaagctgg aaaaagtcat
    32821 gatgaaataa ttgaaaatat agatattggt ggaccatcta tgatacgttc tgctgccaaa
    32881 aactataagg atgtaatagt agtagtagat ataaaagatt atgattctat aatagaaaaa
    32941 ttaaaaacag atacaatgac attagaagac agaaaaaaat tatcatataa agctttctca
    33001 actacaggaa gatatgatgc gttaatatct agttattttg ctggagaagt tggagatacg
    33061 tatcctgaca tattaaattt aactttccaa aaagaacaaa ctttaagata tggagaaaat
    33121 ccacatcaaa atggtttctt atattcacag tcaaatgcaa aaaatccaat cttaaattat
    33181 gagcaattag gcggtaaaga actttctttt aacaacttaa atgatttaca tggatgttta
    33241 gaagtaatga gagaatttaa agatagtgaa gaagtagttt cagtagctat aaaacatgct
    33301 aattcatgtg gggtagggtt aggaaaagat gcttttgaag catatacaaa atgttatgaa
    33361 gcggataagg tttcaatatt tggaggaata gtaggaataa cttctactat agataaagct
    33421 acagcagaga aattaaatga aatattctta gaaatagtag tagcatatga ttttgaacca
    33481 gaagctttag agatacttaa acaaaagaaa aatctaagaa tattaaaatt agctaaaata
    33541 gaaaatagtt tacaaccata tgaaatgaaa tatttagatg gaaaattatt gatacaagat
    33601 agaaacaata tattagcaga aaaaagtgaa aatgtaacaa aggaaaaacc aacagatgca
    33661 caacttaagg acatggaatt tggtatgaga gtagtaaaaa atatgaaatc aaatgcaatt
    33721 gcaatagtaa aaaatggaca aactctagct ttaggttgtg gacaaacatc aagaatttgg
    33781 gcattaaaaa atgctcttga aaataacaaa gataaagatt ttacaggagc tgttttagcg
    33841 tcagacgcat ttttcccatt tgatgattgt gtaacattag ctcatgaata tggaatatca
    33901 gcagtagttc aaccaggtgg ctctataaaa gataaagatt caatagaagc ttgtgacaag
    33961 tatgatatgg ttatggtatt tacaggaata agacacttca aacactaagg aggatattat
    34021 gaagatattg gttgtaggcg gtggcggaag agagcatgct atatgctgga aactaagcaa
    34081 agaaaaaaat gtagaaaaaa tttattgtgc accaggtaat gcaggaattg caaacgtagc
    34141 agaatgtgta aatataggtg atactaatat agaagaactt ttaaaatttg ccaaagaaaa
    34201 tgaaataggt ctgacaatag taggaccaga agtaccacta gttatgggaa tagtagatga
    34261 gtttgaaaaa gaaggattaa gagtatttgg acctaataaa aaatgtgctc aacttgaagg
    34321 aagtaaagct ttttcaaaag aatttatgat aaaacataac atacctactg caaaatataa
    34381 agaatataca aacttagaag aagctatatc agaaatagat agttttggat atccagtagt
    34441 aataaaagcg gacggattag ctgctggaaa aggtgttgta atacctgaaa atagagaaga
    34501 tgctatagca actttaaaag aaatgatgtc agataagaag tttggagctg ctggagataa
    34561 aatagtaatt gaagagtttt taaaaggaat agaaacttca attctagcat ttgtagataa
    34621 tgatacaata gttcctatgg caagtgcaaa agaccataaa aaagtaaaca actatgagca
    34681 aggtccaaac actgggggaa tgggaacttt ttcacctagt gaaatatata cagaagaatt
    34741 agcaaataaa gtaaaagaga cagtattaga aaaaacttta gaaggcttca aaaaagatgg
    34801 acttaatttt aaaggtattt tatttgttgg tcttatgata actgaagatg gagaaaaagt
    34861 acttgaatat aatgtaagat ttggagaccc tgagacacaa tctgttttat ttagattaga
    34921 aacagattta catgagataa tggaagctat actagataat aagttaaaag atatagaaat
    34981 aaactatagt gatgatgaag ctgtgtgtgt tatgcttaca tcaggagggt atccagacag
    35041 ttatgaaaaa gggaaaatta taactggtct tgaaaacttg gatgatgata tagtagtatt
    35101 ccatagtggc actaagatgt ttgatggtaa tttggttacc aatggaggaa gagtaattgg
    35161 tataactgca aagtcaacta cagttaagga tgcagcagaa aaagtttatg aaaacataaa
    35221 gaagataaac tttgaaggaa tgcactatag aacagacata ggaagataat ttttctaaaa
    35281 tggaggataa ggtatgttga atactgaaaa taaagactca atggttagaa gagtattagt
    35341 tgaaaaaaga gaaggttttg accttgaggc taaagcttta aaaaaagact tagtagaaag
    35401 tttacacata gataatatag aaaatttaag aatattaaat agatatgatg tagaaggaat
    35461 atcagaagaa gtttatgaaa atgcagcaaa gacaatattc tctgaaccaa atttagatgt
    35521 agtatattat gaagaaatac caaaattaaa tgatgaaaga gtatttgcaa tagaatttct
    35581 tcctggtcaa tacgaccaaa gaggtgattg ggcagctcaa tgtgtacaaa ttgtaaatca
    35641 agggataaga cctgctatta atacagcaaa agtttatata ttatcaggca aaataactga
    35701 tgaagagttt tctaaaataa aagattactg tataaatcca gtagatagta gagaagcatc
    35761 tttagaaaaa ccagaaacgt taaaaatgga aactgaaata ccaacaacag ttgaagtatt
    35821 agatggattt atagatttag atgaaaatgg attaaaaaca tttgtaagtg aaaaaggatt
    35881 agcaatgact ttaggagact tacaacacgt tcaaaaatac tttaaagata cagaaaagag
    35941 aaatccaact ataacagaaa taaaagtatt agatacttat tggtctgatc attgtagaca
    36001 tactactttt atgactgaaa tagaaaatgt aaagatagaa gatggtaagt ttaatgatat
    36061 agtaaaagaa gcgtatcaaa tgtatcttaa ctcaagagac aatgtatatg tgaacagaca
    36121 taaagatata tgtttaatgg atatagccac tgttgcagta aaagaattaa agaaaaatgg
    36181 gaaattaaat gacctagatg aaagtgaaga aataaatgca tgtagtataa acgtagatgt
    36241 tgaagttgat ggtaagatgg aaaaatatct agtaatgttt aaaaatgaaa ctcacaacca
    36301 tccaactgaa atagaaccat ttggtggagc agctacttgt ttaggaggag ctataagaga
    36361 cccattatca ggaagaagtt atgtttatca agcaatgcgt gttacaggaa gtgctgaccc
    36421 aagaacgact ttagaagata ctttacctgg aaaacttatg caaagaaaaa taactacaga
    36481 agctgctcat ggatatagtt catatggtaa ccaaataggt cttacaactg ggcaagttgc
    36541 agaagtatat gatgaaaact ttgttgcaaa gagaatggaa ataggtgcag ttatagcagc
    36601 agcaccaaaa gagaatgtag taagagaaag acctgaagca ggagatgtaa tcgttcttct
    36661 tggaggaaaa acaggaagag atggctgtgg aggagcaact ggttcatcta aagaacatag
    36721 tgaagagtca atcttaactt gtagtgcaga agttcaaaaa ggagatgctc ctaacgaaag
    36781 aaaaatacaa agattcttta gaaataaaga agttgctcaa atgataaaaa gatgtaatga
    36841 ttttggagca ggtggagttt gtgttgctat aggagagatt gctgatagct tagatataaa
    36901 cttagactta gttcctaaaa aatatgatgg acttgatgga actgagcttg caatttcaga
    36961 gtcacaagag cgtatggcag tagccataaa aaaagaaaat aaagataaat ttatacaatt
    37021 agcagtagaa gaaaacttag aagctacaca tgtagctaca gtaacagata caggatattt
    37081 aagaatgttc tggaatggaa aagcaattgt agatataaat agagagtttt tagatacaaa
    37141 cggagtaaaa caaactactg atgttcatgt tactaaagta gatgaagaaa atacattctt
    37201 tagctctaat gaaatagtaa aagatgttaa gtgtgcaagt atgaaagaca agtttacaaa
    37261 agtactatct gaccttaatg tttgttctca aaaaggatta gtagagatgt ttgataatac
    37321 aattggtggt aatacagtcc ttatgccatt tggaggaaag tatcaagcga ctccaacaca
    37381 aggtatggtt gctaagatac cagtacttgg aggagaaact aatacttcta caataatgac
    37441 ttatggatac aatccaaagg taggaaaatg gagcccattc catggtgcct tatatgcagt
    37501 agtagaatct gtgtgtaaat tggttgctat tggaggaaat tatagtacaa caagacttac
    37561 attccaagag tattttgaaa aacttggtaa caatccagaa aaatggggta aaccattctc
    37621 agcactttta ggagcatttt atgcacaaag taaatttgag atacctgcta taggtggaaa
    37681 agacagtatg tctggtacat ttaaagatat tgaagttcca ccaacattag tttcatttgc
    37741 tgttgacaca gtagatgcga agaaagtagt atctccagaa tttaaaaaag cagactcaaa
    37801 agtagtaatg ttatgtgtaa acaaggcaga aaatgatgtt gtagattttg aggaattaaa
    37861 gagaaactta gataaagtaa gagaacttat acatggaaat aaagttttat caacatatgc
    37921 attaggattt gcaggtgtag gtgaagcaat aagtaaaatg gcttttggta ataaaatagg
    37981 atttaagttt agtgaagagg ctgaaaaagc atttacagat gataaattat ttgaggctag
    38041 ttatggaaat atagtacttg aattagcaaa tgatgattta agtatgttag aaggatacaa
    38101 ttatgttgtg cttggaagta cagttaaaga agctagtata tttatcaaag gtgaagaact
    38161 tgcattagat gaattatata aagcacattg tagtacttta gagcctatat tcccaactaa
    38221 aacagaagaa gtaaaatcaa agatagaaac tataagttat atatcacaag gagaagctaa
    38281 aaaatcatca ttaagtatag ctactccaag agtatttata ccagcattcc ctggaacaaa
    38341 ctgtgaatat gactctgcaa gagcatttga aagagcagga gctaatgcaa gtataagagt
    38401 atttaagaat ttaacttata aagacataga agattctata gatacaatag taaacgaaat
    38461 aaaatcttct caaataataa tgttaccagg tggatttagt gctggtgatg agccagatgg
    38521 ttctggtaaa tttatagcta ctgtatttag aaatcctaga gtgcaagaag ctataaatga
    38581 atttttaact caaaaagatg gactaatgtt aggtatctgt aatggattcc aagtattaat
    38641 taaattaggg ttagttccat atggagaaat aagagttcca agtgaaagtg ctccaacact
    38701 tacttacaat aatattggtc gccatcaagc taagattgct agaactagaa tttcatctaa
    38761 taaatctcct tggttagctc aaactaatgt tggagatatt cataatatag caatttctca
    38821 tggagaaggt aagtttgttg ccagtgaaga tgttatgaga gaattaattg ctaatggaca
    38881 agtagcaact caatatgttg actttaataa tgaagctact tatgatattg agtttaatcc
    38941 aaatggttca ttctatgctg ttgaaggtat aactagtgct gatggtagag tgtttggtaa
    39001 aatgggacat tctgaaagaa ttggtgagga agtatataag aatattatag gtgaaaaaga
    39061 ccaaaagata tttgaatctg gagttaaata ttttagataa agctttgatg tttaattgat
    39121 agctagaata tataatttag atatttagat ttaaagagat gtaataatat gatgataatc
    39181 atttaatatt acatctcttt tttatttatc taagaaataa taagttcaac ctaagttaaa
    39241 atacaagctg tgttataatt aattattaag taaaattgaa tatttattgg agggcaacat
    39301 aattaatgaa aatattaata acagggtcta atggtcaatt aggaaaagaa ttggttaacc
    39361 aattagaagc aattaatcaa agtataaacc aaccaaaata tgttatatta gctacaacaa
    39421 gaagtgattt ggatatatcc aatcagacta atgtagataa ttttattttg cataataaac
    39481 ctgatgtagt tgtaaactgt gcagcatata caaaggttga tgcatgtgaa gataatatcg
    39541 aaattgcata caaaataaat gctttaggag tgagaaatct agcaatagca tcagaaaaag
    39601 ttaatgctaa gcttattcac atatcaactg actatgtatt caatggtttt tctaaatatc
    39661 catataggga agacaataaa actgaaccca attctgtata tggaaaaagt aaacttatgg
    39721 gagaaaaatt tgttgaacaa ttctcacata agtactttat attgagaaca gcatggttat
    39781 atggtaatgg gaataatttt gtaaagacca tgataaaact ttctttagaa aataaagagg
    39841 ttaatgtggt taatgaccag tttggaactc caacaagtac agtagactta gcaaaaatta
    39901 taataagaat catggaaaca gaacattatg gagtttatca tgctacatgt gaaggtgagt
    39961 gtagttggta tgattttgcc aaaaagatat ttaaactaaa aaatatagat ataaaagtaa
    40021 atccaattaa gagtaacgaa tttaaatgta aggctccaag accactatat tctgtattag
    40081 ataattttat gttgaagtta attggtttaa attcttttag aaagtgggaa gaatccattg
    40141 aagaatattt agaaagatat gatataaatg aaaagtaaat gtattatttt gagaaaagaa
    40201 aataagatat tataaaaaat ataaatattt cttttattta tccgataatc ataatatgac
    40261 aaaaacaaat ttaaggatgg aaaaagatga aaggaattat tttagcagga gggtctggta
    40321 caagactata tcctataaca aaatgtatat caaaacaaac acttccaata tacgataaac
    40381 caatgatata ttaccctatg tcggttctta tgttggcagg gatacaagat atattaataa
    40441 tatctacacc aagagatata aaagcatttg aagaattatt tggagatgga agttcactag
    40501 ggattaacat aacatatgca attcaggaga aagcaaatgg aattgcagaa gcgtttataa
    40561 taggagaaga atttatagga aatgacagag tgtctcttgt attaggggat aatatatttt
    40621 atggatatgg atttagtgaa aggttggaga atgcagttaa aagaaaagga gcaactatat
    40681 ttgggtatca tgtaagtaat ccaaattcgt ttggagttgt agaatttgat aaggatttta
    40741 atgtattgtc aatagaagaa aaacctacag ttcctaaatc taattatgct ataccaggat
    40801 tatattttta tgataatgat gttgtagaga tagcaaagct agttgaaccg tcagatagag
    40861 gtgaattaga aataacctct ataaacagtg aatatctaaa acgaggaaaa ctaaaagtgg
    40921 aacttctagg aagaggtatg gcttggcttg atactggtac acataaaggt ctattagatg
    40981 cttctaatta tgttgaagtg gttcaaacaa gacagggatt gtatatagca tgtttagaag
    41041 agattgcata tacaaaagga tatataagta gagaacaatt aatgaaacta gccaagccac
    41101 tgataaaaac tgaatatggt caatatctat taaaattggc tagtgaagat atgtaatatg
    41161 gtagataaaa tttattataa attttattaa gggagtttat tataatggga aaaatcaatg
    41221 ttataaagac taaaatagaa ggattatata tagtcgaacc aaatgtattt tatgatgaaa
    41281 gaggatattt tatggaaagc tataataaaa aggattttat agagcagggt cttgatatgt
    41341 gttttattca agataatgag tctagctcaa gaaaaggcgt tttaagagga atgcattttc
    41401 aggttaaata tcctcaagga aaattagtca gagttacaga gggagaagtt ttcgatgttg
    41461 cagttgattt aaggaaagat tcacaaacat ttgggaaatg ggaaggtgta atattgtctg
    41521 ataagaataa aaaacaattt tatattccag aagggtttgc acatggattt ttagttttat
    41581 cagaaaaagc agtatttagt tacaaatgta caaattatta ttacccagaa tttgagaatg
    41641 gttttatatg gaatgataag caaatatcta ttgagtggcc tttaaaagga attgaagaaa
    41701 tatttttatc agaaaaggac aaaaaacaaa aaagttttaa agatatttgt acaagacgtt
    41761 aataaaaatt tattagaatt aacatttagg agaaaaaata tgaaaaaaat actgataact
    41821 ggaggagcag gatttattgg gagtaatttt atacattata tgcttaacaa gtatgtagat
    41881 tattttattg ttaacctaga cttgttgact tatgctggta atttagagac attaatagaa
    41941 atacaagaca attccaatta taaatttgtt aaaggagata tttcaaatag ggagcaggtg
    42001 tataagctat ttgaagaaga aaggtttgat atagttgtta actttgccgc tgaatctcat
    42061 gtagataggt cagttgagaa tcctgattta tttattaaaa caaacattat aggaacacaa
    42121 gtacttttag atgcatctat aaaatatgga gttaaaaggt atcatcaaat atctacagac
    42181 gaagtgtatg gtgatttacc aattgataga aaggatttat tctttacaga acaatcttct
    42241 atcaatccaa gttctccata ttcagcttct aaagcatcag cagatttatt ggtaaattct
    42301 tattatagaa catatggatt gttaacaact atatctaggt gttccaacaa ttatgggcca
    42361 tatcattttc ctgaaaaatt gattcctctt atgattttaa atgctttgga aaacaaacaa
    42421 ttgccagtat atgggaatgg ggaaaatgta agagattggt tacatgtata tgaccattgt
    42481 actgctatag acttaataat acataaagga aatatagggg agatttacaa tataggaggt
    42541 cataatgaaa gaagtaatct agaagtagta aaagtgatat taaatctttt aggtaaatct
    42601 gaagagttaa tatcctatgt aaatgataga cctggtcatg atttaagata tgctattgat
    42661 gcaactaaaa tagagaatga gttaggttgg aaggcaaaat atgactttga tttgggtata
    42721 aaagaaactg ttaagtggta tatagaaaat gaatcttggt ggaaagctgt tttatcagta
    42781 taactttaaa atttagaaag gggcgtttta tattgaatga gataagttca ctaattaata
    42841 tattagcatt gactcaatta tctggtagta atactacaaa tcaatgtaat tgtacatata
    42901 ataattcatc tggatttgat atgattatga tgaccttgtt aaaagcatta tcacaaaata
    42961 atcaacatac ttctaatagc tacaatttat tgtctaattc tgagaattta tttaatgaac
    43021 ttgataatgt agtaaataat accactagta gatttataga tgttgataca aaagataaaa
    43081 atgtaaagtt gagaattgaa aatgctgtag atcaagcatc aaaaaaatat aacgtagatt
    43141 caaatcttat aaaagcaata attaaagttg aatcagattt taatccaaat accgtttctt
    43201 ctgctggtgc taagggtctt atgcaactta tgccagaaaa ctgcagagac ttaggagtta
    43261 cagacccttt taatatagaa cagaatatag atgctggaac tagacatatt aaggaataca
    43321 ttgatatgtt tggagggagc atagaaatgg gattgatggc atataatggt ggtcctggaa
    43381 gaatgaaaag tagaggagta gaatcaatct ctgacctgta taaaatgcca aaagaaacac
    43441 aaaattatat tcctaaggtt atgaaatatt atagagggta ggctttatta tgggaataaa
    43501 tatagataag ctaaacataa gtccaagtat aaatatggag gttaaaaaac taaaagagag
    43561 taataaattt tctgacaaat taaactcttt aaaacaatct tcttcattgg aagatataaa
    43621 acgaaatttg gaaagtgtaa aaaaggcagg agaaaggctt gtacttactc aaaactatgg
    43681 cgatattagt atatataaaa atgcagtaaa agaataccta aaatctgttg tagataatat
    43741 gtattcactt gataaaaata gtagcttttg ggagcatcag tattataaga atgtaaagat
    43801 tgttgatagt aaacttgagg atatgactag taagttaatt agtgaagaaa aagaaaacat
    43861 agatattgtt tctactgttg acatgataca aggcttattg atagatatgt atagataagg
    43921 ttatacaagg agaaaaattt tatgaatagc aaaaaagagg atatatatga attgaatttt
    43981 gggcaattaa tagaagaaaa atctggtgtt atagaagaaa atcaaaatgt ttttaatgca
    44041 aaagtaaatg tagctgtttc tataggaaat acaacagaaa caataaaaaa tatattagat
    44101 ttaaaagaag gctctataat aaaattaagt aagaatgtgg atgaaaaatt agatatatat
    44161 gcaaacgata gattgtttgc ttatggagaa agcattatta caaatgagag gttatctgta
    44221 agattatcta aaatagaaga tccagatgta aattagcatt ttaaatagta tccctttagg
    44281 ctattctgta tgagtttagc taaggggata tttttataaa aaatttaaaa tatgtgtgaa
    44341 ttatataaat atttttattt tatgagcgat aactaaatta taagtatgta attttggagg
    44401 tgaaactatg gatattaaaa atgtaagttc aaacattgta gtgaataatt atttaaagaa
    44461 tacatctggt ataaaaaaag tggaagaggt taaaaaaatt aataaaacag acaagataga
    44521 gttatctaaa tttgcaaagg aaatggcaag tgtgtcaaat atgagtgatg agaatattag
    44581 aaataaaaaa gtcgatgaaa taaaaaatgc attagaaaat ggaacttaca aaataaaacc
    44641 aaaagaaatt gcgaaaaaaa tgatagagga tatgcgagga taaagtcatg attacagact
    44701 taaaagttat aatatatgaa gaaaaaaata taattagtaa gatgatggaa ttattaaaag
    44761 aacaatttga ttatatagtt gaaaaagact tagaaaattt aaatatgtta aatcctaaac
    44821 tagaagatat tagcagggag cttgcatcca tagaaataaa gagaagacaa ctttttggtg
    44881 atgatatttc aatgagtgaa gttgttgaaa atagtaatga tggatactta aaagaaatat
    44941 atacagactt gaaacacatt cttgctttag ctaaaaatca acaagaatca aatgattctc
    45001 taataaagaa agaacttatt tttacgaaaa aaatgattaa tttcataaaa ccagctgata
    45061 agaaaactac aacttataat tcttatggaa atattagaaa ataaatatta ggaggagaaa
    45121 tatggcagga ttattttcca ttttaaatac agctaattca ggaatgaatg cgcaccaaaa
    45181 agcaatacag acaatttctc ataatataag taatctagat acagacggtt attctaggca
    45241 aaggaccgag tttgccacaa atagtccaat gtatatgcct agtttgagta attcaattgc
    45301 aagaggacaa ttagggactg gagtacatgt aacggatgta acaagagcta gaaatagttt
    45361 ttatgattac caatttaggg cagaagctca taagtatggt aagacagttt caaaatatga
    45421 ttattacaat actatagaaa ctatactaaa tgaaccatca gactatggaa tatctgcagg
    45481 tatagatgat ttttttaaag gatgggagtc attatcaaag gatataaata gtgacagtaa
    45541 aaaaagtcta gtggtagaaa acgcatctaa tttagcaact ttaataagtg aaagttataa
    45601 gaagcttgat aagttaaaag aagatataga ttctaatata gacactgaga taaaacaaat
    45661 aaatgacatg ttaaaaagtc ttgaagattt aaataaatct attgatatta tatctggttc
    45721 tggctctaca ccaaatgatt tacttgatga aagagataga attttagata acttgagttt
    45781 taaactagac tttgaaaata gtgatgttaa aaatatgctt gcagatggta aattagaact
    45841 taatgaatta aaaaatccag atggaacttg gaaaacaggt atatcaggga ccttacaagg
    45901 cttgtttgaa atgcatggta agattgatac atataaaagc gatttaaaag atgtatctga
    45961 tggattagct aaacagataa atgatgttta taatacgtct gcaggaataa cagtaacaga
    46021 tttttttata acttcaaatg tagatggtga agatattata aagataaatc cagctataaa
    46081 gagtaaccca aatgaattaa agcttactgc tgatgaagct tcaaaaattg ctaagttaaa
    46141 ggatgaaaaa atagatattg gggtggctgg tggaaaagta agtacaataa gtgaccacta
    46201 taaagccttt gcagaatcaa tcggtttaga ttctcaaaaa gtaaatcaag atgaagcaaa
    46261 tcaacgtaaa attataaata atattgataa ctcaagaatg agtgtttcag gtgtatcttt
    46321 ggatgaagaa atgacagagc ttataaaaat acaacgttca tatcaagcta gtgcaaaagt
    46381 tatgtcaaca gctgtgcaac tactggatgt tgtaataaat ggaataattt aatagaaggg
    46441 ggaatttata atgagagtca gtacaggtat gatgtctagc agctatctaa actcactaca
    46501 agataatcta caaagattgg ataaggttaa tagacaagtt aatacaacaa aagaaataaa
    46561 caagctatca gataatccat ataaagcaat aaagatatta aactcaaaaa gtgaaataaa
    46621 gactatggag acttatgttg agaattgtaa agatacaggg aattggttag aaactactga
    46681 tactgcatta gatcagcttg gaaaattatt atcagacata aagaaaactt tggtggcaag
    46741 tggtgatggt gcatactcag atgatgaatt aaagactctt agtaatgaaa ctaatgaaaa
    46801 aatgaaagaa attgcaaatg ctttaaatgc aacacatgaa gggaagtaca tattttcagg
    46861 ttcaaatacg ggggtaccac ctgttgaatg tatagataat gctgatggtt cagtgagttt
    46921 aaagtttaat tctagtctaa atcttaataa attaaatgac tctttgagta tggatatagc
    46981 tcaaggaata agtgtagatt ataacgttaa attgagtagc cttggatttg acccaacaaa
    47041 aactgaccca tttgaagagt taaataacat atcaaaaaaa ttagcaaatc cagatgctga
    47101 tgctataaaa gagcttacaa caacttgttt gggcgatatg gaacaactaa tagaaaatac
    47161 agtaaatgtt aggtctatat atgggacaaa agcaaataca gtagaggcta tgaaggaaaa
    47221 aaacgatgat agtttgatac agttaaagga tattttgtca caaaatgaag aaatagatta
    47281 tggtgaaaaa cttgttcaat taaaggcagc agagttaact tatcaagctt cactacaaac
    47341 aggaggaaag ttatttaatg tatctatatt agattacata taataaaata acgcagatat
    47401 gatgaaggtt acattaaaaa aaggtatatt aggttttgaa aatttaaaag aatatgagct
    47461 tttagatata gaagataatg attatttaaa agaatttaat tctactgaag aagaaggtat
    47521 aggatttgtt gttgtatctc catttgagat tatcaatgaa tatgaaatag ttttaaatca
    47581 ggaaacaata gaaaaactag aagtgaaatc tccaaaggat gttgtattat ttaatataat
    47641 tactcttggt cagacatttg aagaatcaac tgtgaacatg aaagctccta tagtgataaa
    47701 tataaaaact aattttggaa tgcaaataat tttacaggat gataaatatt caataaggca
    47761 tccattattg agaggtgata atggatgcta gtaatttcaa gaaaaaaaga tgaagcagta
    47821 cttataggag aaagcataga agtaaaaatt gtaggagtag atggaaacaa tgtaaaacta
    47881 gctatatcag cgcctaacaa tataagtata ttaagaaaag aaatatacga aaaagtaaag
    47941 agtgagaata taaaagctac aaataaaaat ataaaaattt taaagtcatt aaaataataa
    48001 gcttggagga agtaatatat gtatggagaa aatccttata attcttataa acaaaatgct
    48061 gtttttatgg catcaaagga acaattgtta cttatgcttg ttgatggagc agtaaaatat
    48121 accaaaatag ccaaaacagc tatcttagat aaaaatacac aaaaagcgca tagagagctt
    48181 ataagagttc aggatatatt tacagaacta atggttacat tagaccaaaa tgcaggtcaa
    48241 tgggcaaagg atatgtacag agtttatgat tttgtaagat atgaactttc aagagctaat
    48301 attagaaaag acattcaaat aatagataat gtgttgcctg taatagaaca aattaaagat
    48361 acttggcatg aagctgataa aaaaagtaaa gaagaaagaa gtaggtacaa ataatggaat
    48421 taaatttagc tgaattagat aagttatcaa aagaggaatt actattgatg cttgtagatg
    48481 gaacagtaaa atataccaat atatccaaag aagctctttt aaataacgac tatttgaaag
    48541 cacataatga acttgtaaga gttcaaaata tatttacaga actaatgact acattagacc
    48601 aagatgcagg tcaatgggca aaggatatgt ataaagttta tgaatttata aaatctgaat
    48661 tagcaatagc tgatgaaaat aaagatatta aaataattga tgatatattg ccaatagtaa
    48721 aacaaattag agatacatgg catgaagttt acaatcaact aaaaatataa ttggatggag
    48781 ggataaaggt atgtcaagta taagtcctat aagagttaca ggtctttcag gaaattttga
    48841 tatggaaggc ataatcgaag ctagtatgat tagagacaag gaaaaagttg ataaagcaaa
    48901 acaagaacaa caaatcgtta aatggaagca agaaatatat agaaatgtta tacaagaatc
    48961 aaaagatctt tatgataaat atctaagcgt aaattctcct aatagtatag taagtgaaaa
    49021 agcatactct tctacaagaa taaccagttc tgatgaaagt attatagtag caaaaggctc
    49081 agctggtgca gaaaaaataa attatcaatt tgcagtttct caaatggctg aaccagcaaa
    49141 atttactatt aaattaaatt caagtgaacc tattgttcaa cagtttcctc caaatgctag
    49201 tggagcgagt tctttaacta taggagatgt aaatatacca atatctgaac aagatactac
    49261 aagtactatt gtaagtaaga taaactccct ttgcgcagat aatgatataa aggcttctta
    49321 tagtgagatg acaggtgaat tgattatttc aagaaaacag actggttcgt catcagacat
    49381 taatttaaga gtaattggaa atgacaattt agctcagcaa attgctaatg ataatggtat
    49441 cacatttata aatgatgctg gtggaaacaa agtggcaaat gtatatggaa agaatctaga
    49501 agctgatgta actgatgaac atggaagagt aactcatata agtaaagaac aaaattcatt
    49561 taatatagat aatattgact ataatgtaaa ttcaaaagga actgcaaagt tgacttctgt
    49621 cactgatact gaagaagctg ttaaaaatat gcaagcattt gtggatgatt ataataaact
    49681 gatggacaag gtctatggtt tagttactac taaaaaacca aaagattatc cgcctcttac
    49741 agatgcccaa aaagaagata tgacaactga agaaatagaa aaatgggaaa agaaagctaa
    49801 agaaggtata cttagaaatg atgatgagtt aagaggtttt gttgaagata ttcaatctgc
    49861 attttttgga gatggaaaaa atattattgc attaagaaaa ctaggtatca atgaaagtga
    49921 aaattacaat aaaaaaggtc aaatatcatt taatgcagat actttttcaa aggctcttat
    49981 agatgatagt gataaggtat acaaaacact agcaggttat tcttcgaatt atgatgataa
    50041 gggaatgttt gaaaagctaa aagatattgt atatgaatat tctggaagtt caacttctaa
    50101 acttcctaaa aaagcaggta tagaaaaaac tgcttctgct agtgaaaatg tatattcaaa
    50161 acaaattgca gagcaagaaa gaaatataag caggttagtt gaaaaaatga atgataaaga
    50221 gaaaagactt tatgctaaat attcagcctt agaatctttg ttgaatcagt attcttccca
    50281 aatgaattat ttctcacaag cacagggtaa ttaattatgg ataaattaaa agaaaaatta
    50341 aatttatata aagatatatc tttacaaatt ataaatttga ttgaaaagga agaatatata
    50401 aagataagta ttaagttagg tgaaagacaa gagattataa atagtgtaag tgaaattgat
    50461 agaaatgatt ttatacagct ttataataga atggaattaa ttgaaattga tagcaggata
    50521 agagatattt tacaaggaca attattagaa gtaaaaaaag aattacatga gtataagctt
    50581 actaaacaag tgaacacaat gtattataac ttaaatagag aaaaagtaaa tatttttaat
    50641 aaaaaagtat aaagttatgt accatcttgt ccgattatat aaatgaagaa ttaataaaaa
    50701 agattaaaag tagaaatgac aaggatgtca actatactaa ggagggtaaa ataatgagag
    50761 ttaatacaaa tgtaagtgct ttgatagcaa ataaccaaat gggaagaaac gtaaatgcac
    50821 aaagtaagtc tatggagaag ctatcttctg gtgttagaat taagagagct gctgacgatg
    50881 ctgctgggct tgctatatct gagaaaatga gagctcaaat aaaaggttta gaccaagcag
    50941 gaagaaacgt tcaagatggt atatctgttg tacaaacagc agaaggtgca ttagaagaaa
    51001 ctggtaacat actacaaaga atgagaactt tatcagtaca atcttcaaat gaaactaata
    51061 ctgctgaaga aagacaaaag atagctgatg agttactaca attaaaagat gaagttgaaa
    51121 gaatatcaag ctcaattgag tttaatggta agaaattact tgatggaagt tctacagaaa
    51181 taagattaca agttggagca aactttggaa caaatgttgc aggaacatct aacaacaaca
    51241 atgaaataaa agttgctcta gtaaatactt caagtataat gagtaaagca ggtataacaa
    51301 gttctacaat agcaagctta aatgcagatg gaacttctgg aacaaatgca gctaaacaaa
    51361 tggtatctag cttagatgta gctcttaaag aattaaatac atcaagagca aaattagggg
    51421 cacaacaaaa tagactagaa tcaactcaaa acaacttaaa taatactata gaaaatgtta
    51481 cagcagctga atcaagaata agagatacag atgttgcttc agaaatggtt aacttatcta
    51541 aaatgaatat attagttcaa gcatcacagt caatgcttgc tcaagctaat caacaaccac
    51601 aaggagtttt acaattatta ggataaaata attttgtata ttggatatag aagaaagggt
    51661 aaggctttgc tttactcctt tcttttttta caaatatact tttgaaagga aaatttatat
    51721 gttacttagt atagttatga ttgttaaaaa tgaagaaaaa atacttgaaa aaactttaaa
    51781 atcattaacc acattaagaa attctataga aagtgaactt ataatagtcg atacaggttc
    51841 tacagatgat actataaaaa tatctaagaa gtacacggaa aaagtgtatt ttcataattg
    51901 gaataatgat ttttctagta tgagaaatat atcaatatcc tatgctaaag gtgaatggtt
    51961 acttatatta gatgcagatg aaatactaat tgattcttca actattgtaa aattttttaa
    52021 agatggtctt gataaaaaat ttaattctgc ttcaatacaa ttaaaaaatc tttactcata
    52081 tgataaaaaa ttatatggat attgttcggt gcttagactt tttaaaaacg ttgattttag
    52141 ataccatggt aaagtacatg aacaaccaat atacaaaaac cctatattta ataatgttgc
    52201 tgattttgaa cattatggat atttgtttga agatgaagaa ataagattaa ataaggtgaa
    52261 aagaaatgaa aaactattat ttcaagagtt aaaagagaat gataattctc catatacaaa
    52321 ctatcaatta gggaaaaatt ttattatatt aggaaaatat caggaggctt tatactatct
    52381 tgagaaatct tatgaattat attctaaact agaattggca cctggttact tgataacaag
    52441 ccttgtaaaa acatatttat atttaggaaa acataagaag tgtgaaagat tgtgcttaaa
    52501 gtatattaag aaagacatga ataatattga tatatattat tatattgcac aagcacaagt
    52561 agatttagga aagtatgaaa atagtataga ttcgtataag agatatattt acttgttaga
    52621 taattatgaa ataagtacac aagcaaatag tttattttca gacactgata ctgtagggct
    52681 tagagatgag gctattatta cactgataaa gatttattat aagcttgaaa aatatgagtt
    52741 tgtaatttca gagtataata acattgaaga tgtggaaaag aaaaagaatg tgtactttag
    52801 cttatttatg tcactatata aattgaatag atttgaagat ataaaaaatt attataaaga
    52861 agtttcaaat tctaaaattg aaaaaaatag tttttataga aatatagaag aagttataaa
    52921 taatattaaa gaggatgaaa aagagtatgt atataaaatc ctttgtgaca ttgaaggtaa
    52981 ttatggcatt ttaaatcaaa taaggataaa taaatgtgta tctatagata aatgtaaaga
    53041 aatactcaat gaagaaaagg aaataattta tgcatatatt ataaaaatag cttttgaaga
    53101 gaagattgat ttactagata ttttttatga aatggaatat gtatggatag agaaatatct
    53161 aaaatatctt ttagcattag aaagaagttt taatttagta ctatataagt acataattag
    53221 tcgtccaaat acatcagata tggaaaagat aagagtatat aaagctatat ctaaagtagt
    53281 tttagaaagt ataagcttaa gtgatgaaaa atataaggag attttttatc tttatataat
    53341 gtattcttat aagtacataa gatacatata tagcaattta agtgattttg aattaataaa
    53401 atttgtttgt aatgatttag ataagttcac tatagaattt aagcatctac ttgatattaa
    53461 aaataaagta aataatgcta atgaaaaatt agagtatata catagtatga ggaaattact
    53521 aaatgagtat cctttttata ataaaataat aaaaatactt ataggtgaat tagaagaaaa
    53581 tataaaagaa agtgaagaat ttaaggattt aaaagatagt tttattatta gtatagaaaa
    53641 tatgattgaa actggcaaaa taaatgatgc gaaattattg gtagatgact attcaaaaac
    53701 atttgtggat gatataaaga ttttaaatat aaagggaatt ttatacatgt ttgaaaataa
    53761 gcttgataaa gctgatttta tgttaaaaaa ggcattatca ttagatgtag aaaatgaaga
    53821 tactatatat aatatagaat acttaaaaag tttaagataa gtcaaaaata ggagaattga
    53881 agaatgaata taccaatagt ggttaatgaa aaatctaaag acataataaa agattggcaa
    53941 tatacatgta caaatgaaga tataaaattt acaagcataa taatattgac atataatcaa
    54001 atagaatata ctaaattatg tattgaaagt attaggaaat ttaccccaaa gaataaatat
    54061 gaaataataa taattgataa taactcttct gatggaacag ttgagtggtt aaagggacaa
    54121 tctgatataa aatctgtgta taataacaaa aacttaggat ttccaaaagg atgcaatcaa
    54181 ggtattgagt tagctactgg tgaaaacata ttgttgttaa ataatgatgt aatagttact
    54241 ccaaattggc tctataattt gaataaagct ttatggtctc aagataatat aggtgctgta
    54301 ggacctatta cgaatgcatg ttcatattat cagcaaattg atgttgatta taagtcaatt
    54361 gaggacatgt ttatatttgc acacaaaata aataaatcta atattaacct atggaaatat
    54421 agattgaagt tagttggatt ttgcatgtta ataaaaaaaa ctgttctgga taatgtaggg
    54481 gttttagatg aattattcac acctggaaat tttgaagatg atgatttatc ttttaggatg
    54541 atagagtcag gatataagtt attattatgc caggatactt ttatacacca ttttggaagt
    54601 gtgtctttta aagaaaatcg taataaatat acagatataa tgtatattaa tagaagaaaa
    54661 tttgaaacta agtggggatt taatccagaa tatagtggaa tgataagaac agatttgatt
    54721 tctaaaatag atgatagttt ggaggcaaat attaatgttt tagaaatagg atgcggtata
    54781 ggggctactc ttttagaaat aaaaaatata tataaaaatg catcaatata tggaatagaa
    54841 attagtgagt cagcaggtaa acttgcaaag tatagtgcag atgtacttat agcaaatata
    54901 gaaaaagtaa aattggatta taaaaaagat ttttttgatt atataatatt gggagatgta
    54961 cttgaacatt tgattgaccc atggaagata gtcaatgaat tgaaacaata tctaaagaag
    55021 gatggttatc tggtatctag tataccaaat attatgcatg tttctgtttt aagaaatctt
    55081 gtgttaggta attttacata tgaagattca ggaattttgg acaagactca tttaaggttt
    55141 tttacattaa atgagataac aaaaatgttt aatgatagta attacataat taatagtatt
    55201 acagctacta gtatgccact ttcggaaatt gataatagct tcattgatac aatatgtaag
    55261 gaaagctgta aagaaatgag acaacagtac gaatcttatc aatatatagt aaaagcctct
    55321 aaaatcctag atactaaaag atattctagt attagtatga ttaatttaaa atacgcttta
    55381 atgagaattg ataatgaatt agatgtagat agtaatttaa gttatatatt tgataattat
    55441 aatataactg aaccatgttt tgttgatgat attgaatatc taattgatac atttgtgata
    55501 aataaagcaa ctctattaaa tagattagga gttgaggcat ttaacagagg tctaggagat
    55561 tttagtataa acatgtttat gacagtatta gagatggata gagatgatat tgactcaata
    55621 tataatataa caagtgtatt atgtgaatta gaagaatttg atactgctta taatttaatt
    55681 aaaaatagta gcgaaaaagt acagagtgaa agtgatatac aagagatact ttgttttatt
    55741 gaggggaaga aatatgaata atgataagat atgttttata acctgtgtaa atgatgaaag
    55801 gctttatgaa gaatgtcttg tatatataaa tagcctagac attcctaaaa actttagtat
    55861 agaaacagta gctttaagag gagccgattc tattgcctct gcttataata aagctatgag
    55921 agaaacagat tctaagtata aggtatactt acatcaagat acttttataa ttaataaaaa
    55981 tttcatacat gatattttag agatatttaa aaaaaataca gatgtcggaa tgattggtgt
    56041 agctggagta aaagatatgc ctgtgaatgg aatttggtgg gattctgata aaaaagcagg
    56101 gaaagtatat gatagtcata caggtaaaat ggatatttat gaatttagta actttgaaga
    56161 atgttttaat gaagttatgg ctatagatgg attaattatg attactcaat atgatgtatt
    56221 ttggagagag gataagtttg atgggtggca tttttatgat ttgtcacaga gtatggagtt
    56281 tagacaaaat aaatacaaag ttattgtacc aaaacaaaat aagccttggt gtatgcatga
    56341 ttctggagta gtaaatataa ataatggata tgatgaatat aggataaaat ttgtaaaaga
    56401 gtacataaat cttgatttat atagagaaaa tttttatgaa tttgataaga agaaagcaag
    56461 taccatactt aagagtaata agcatgtaat ggattgtatg aaagaggagc ttacagaaca
    56521 attaagatta aagaattaca aggatgtttt aggtattgtt tctaaaattg ctcatttttc
    56581 tgtttttaat catactggtt tgtattcttg tgaagaatgt gagagagcat tgctagaatg
    56641 tgcaaataat ttaccagaaa tagaaaatac gaaattattt acaaaaaaag atttaaaaag
    56701 aagaaaagta ttacatgttt taagtgaagg atattcaaca ggaggtcata caagactagt
    56761 taaaaattgg attgaaattg acaaagactc tgttcattca gttgtaacta catggcaatc
    56821 agatacactg ccagactggt tattaaaagc aataaatgat agtggtggcg aagttatatc
    56881 tttggattta atttcagatg attatttaaa aagagctgta gctttgagga aaatagctta
    56941 tgactgggct gatatagtta ttttatatgt tcatatgtgg gatacaatac ctgttgttgc
    57001 ttttggagtt gatgggaaaa tacctatttt acatgttaat cactatgata gtactttttg
    57061 gttagggaca agtattgcag atattgtcat tgattttagc gaaactagtc aattaattac
    57121 attagataaa agaggcatag ataaatctga aatattacca atgccattaa aagaaaaaga
    57181 agtattttca aaatctgaag ttagaaaacg atataattta gaggatgatg tgacaataat
    57241 attgactatt gctagtgaaa gaaaatacaa tagtgtaaat gatattaatt actttaaaat
    57301 tgctaaaaca ttgattgaaa atacagataa tacaattatt attgttgtag gagttgatac
    57361 agagaatctc tattggaaaa ctttaagtaa tgttacagat aatagagtta tatgttttgg
    57421 aacaacagca aatatagatg atttttataa gatttcggat atatacctag agtctttcat
    57481 ggtaggaagt catacttcaa aattagatgc aattttatat gatttaattc ctgttaagtt
    57541 taaaaatgaa gtttcaccaa tttttacaaa tatgtggaat tttatagata agtttgatta
    57601 taagaatata aaagaggtta tagacatgat aagaaggtac aatactagtg aatttgacag
    57661 ggataagata aaagcaaaac aatcggaaat aaaacatttt gttatagata aacattgtat
    57721 tgaaataaaa aactatatag acaaaatata taatgaagtt aatcatcata aagttaagta
    57781 tgatttatat aaaaatgatg aaatcgagga ctatcagctt tttgtagcat tatatggcta
    57841 tatggtaaaa aataaaaaca tgtcataggt ggtaaaatga acaatattca tatcaataaa
    57901 tttgaaaaaa gctgtgaaaa aaactgggat tattttgtag aaaataaatc tataaatggt
    57961 acatttctgc aaactagaaa tttccttaat tatcatgagg agggaaaatt cactgaccac
    58021 tcattaataa tatgtaaagg tgaagaaata ctagcagtcg tacctgcatg tgaaacttat
    58081 gaagatggtg aaagaatatt tttttctcat ttagggagta cttttggtgg cattataata
    58141 agaagtggtt ttaatgatat taggcatgta aatgccatta caaataagtt agaaatatat
    58201 ttacaggaac aaaatttcga taaagtatat ttaaaaaata catcaagaat attttgtaaa
    58261 gaggatactt cattactaga atatttttta tttaagaata attacatgtt ttatgatgaa
    58321 ttaagtatgt ttttagaatt aagtaagtat aaagaagata tattagccaa ttttaaatac
    58381 agtaaaagaa gagattttaa atattctttg aagaataatc ttacctttaa aagactgtat
    58441 agtgatgaag atatagtttg tttttttaat ttactttcta attgtttaga tagtaaagga
    58501 gctaaagtaa tacattcagt agaagaactt ttggagttta aaaataatag attaaaagat
    58561 attgtagaat tttatgcagt atatattgat gacattatga tttgtgggag catggtattt
    58621 aactttcaag aaagagttta tcatactcaa tatctagcat caaattcgac atataataaa
    58681 tattatccaa tgatattttt atactataat ctaataaaag atgctttaga gagaaatttt
    58741 aagtatcttt cttttggaat tagcactcta aatagaggca aatacttaga tgagggttta
    58801 gctacattta aagaaggttt tggttcaagt gcaatttgta acagaacata ctataaaaat
    58861 ataaataagt gagaatcatg ttttatagag tttttagagt atagtaaata tctagtacta
    58921 taatattaca taaaaataat tagtttataa agaaggtttg tatatgaaaa gattaatgat
    58981 tttaggctca attaagtatt ttgagaatat tgttatgtca gctaaaagag agggaatata
    59041 cacaattgtt tgtgataata gaattgatac tcctgcgaaa aagatttgtg atgaagcaat
    59101 agatgttgat gtatttgatt ttgataaaat aaagaatata gctatagata aaaagataga
    59161 tggaatttta actggattta cagattcttt aatgaagcca tatgtttata ttgcaaatga
    59221 actaaatttg ccatgtgtga tttcttgcaa tcaacttgaa tctgtaacta ataaggctgt
    59281 tatgaaaaat tattttaaaa ataatggaat acctacaact gattattgta ttattgaaag
    59341 tttgaaagat ttagaaaaaa tacaaggatt aaattttcca cttgtaataa aacctgttga
    59401 tggttatggt tcaagaggtg tatattttgt atcaaacata gaagaattaa tagaaaaatt
    59461 tgaatattct aaggaagcct cgcaagatgg acaagtaata attgaggaat tttatgaaag
    59521 tgaggaaata caaggactag cttgggttca taatggagag tcacatgtat tttatattgg
    59581 agatagagag cttgtaaata tacatacagg aagagctgga aaaccagata gattaattta
    59641 tccatcaaag tattgttttc aatatgaaga agaaataaaa tccatatatc aaaatataac
    59701 gcaggctttt ggcataaaaa atggacctct ttatgtacaa atgttagtgg gagcatatgg
    59761 aataaaggta tctgaagtaa tgccaagatt acctggaggt tgtgactata tggctatttc
    59821 tcaaataaca gatttagaca taggaagttt atttgcaaac tttagtgtga ataatgaagt
    59881 aaattttgat gaaataaaga aatataatat gcatctatca aaatgtgtat atgcattacc
    59941 aatatacata agacctggca caataaaaaa gataaataat atagataaaa tagaaaactt
    60001 agattatgtc actcaatttc tattactagt aaaagaagga gatacaatag gattatatga
    60061 agatgagaga caggattgtg gaagagttta tggagtagca aataatgtat ttgatgcaaa
    60121 tataaagaag aaatgtattc atgaaatgat agaaatactt gatgaaaata atgaaaatat
    60181 gatagaaaat ttttaaataa gggagtatga tagatatgaa gctaataact catgaacaaa
    60241 ttatgtcatt aaacatttct cagaaacaag gatacgagtg gataaaagaa tcctttttaa
    60301 caaaaaaaga ttgtcaatta ccaccaaaaa taagcatggt agaagaaggg catgtatttt
    60361 acaatgtaat gccgtcccta aatcataatc ataagattgg tggaataaaa atggttacaa
    60421 gattgccagg gagaaatcca agtttaaatt cagagatatt tctatataat atggaagatg
    60481 ctaaattaaa ggctatttta gatggcaatt atattacagc ttttaggact gctgctgttg
    60541 ctgccttggc tatcgataca tttgctaaaa aagattttaa tactattgct attatgggtt
    60601 taggtgtaac tgcttctgct ataatgaata tgctttcata tactcttaaa gataaaaata
    60661 taaatataaa gttacttaaa tataaagacc aagcagagtc gtttgtaaaa aagtatgaaa
    60721 aaaatgaaca atttagtttt gaaatatgtg acacacatga agaacttatt gtagattcag
    60781 atgtagttat ttctactgtg acatatacag atgaatattt atcagaattt aaatggttta
    60841 aaaaaggtgc tttattatta cctgtacatg ttatgggatt tggaaatgtt gattatctat
    60901 ttgataaagt atatttggat gattataatc atttgaaaca tctagaaaat atagatagat
    60961 ataaatattc ttgtgaattt tatgatgttt taagtaaaca agctaaagga agagaaaatg
    61021 atgatgagag aataatatct tacaatatag gtttgtcaat acatgatata ttatttgcaa
    61081 ataaaattta cgaattgtgt gaaacactaa gctgtgaaga aatagaatta aaagaaccaa
    61141 aagaaaaata ttggatatag tttatttcat tcatatttac aatagtttat tgtaaatatg
    61201 aatatgaagt aatatattgt tagaaaatta gcatgaatag gatagataaa tttatggata
    61261 aatataattt aattaggttc gtatctattg gaaacgaata tataggaaat ttagttgcat
    61321 tagaatcaaa taaagcaata ccttttgata taaagagagt ttattacata tatgatgtac
    61381 caaacaatgt aaagagagga tttcatgcac acaaaaactt ggaacaagta ctagtttgcc
    61441 taaatggaag tgtgaagata aaatgttatg atggtgtaaa agagactata tttaatttac
    61501 ataaaaatga tgttggatta tatataggaa cgggagtgtg gcatgagatg tttgattttg
    61561 atgaaaaaac tgttttaatt tctatagcat ctgaacatta taatgaagaa gattatatta
    61621 gagactataa agaattttta aagtatttaa ataatatgta gttattatac agttagtgat
    61681 gtattttctt aattaaataa atttaagata gccacataga gaatagatag attaggatgt
    61741 gaatttattg ttggttaaat ttaataattt attaaaacag ttccaaaccc ataaagaaga
    61801 atatgaacag aagattaagg atgtaatgga aagtggatat tatatccttg gaaatgaagt
    61861 aaagcatttt gaagaagaat ttgctaacta tgtaggtagc aaatattgtg ttggtttagc
    61921 cagtggttta gatgcactga taattgcttt tagagcatta ggaataagcg aaggtgatga
    61981 agtaatcgtg caatcaaata catatattgc tagtgttatg ggaatcagta taaatggagc
    62041 aacaccagta tttgtagaac caaatgaata ttataatata gattcatcta agatagaaga
    62101 aaagataaat agcaaaacaa aagctatatt agtagtgcat ttatatggtc aagcaagtaa
    62161 tatgaaagat atacaagaat tatgtgataa gtataacttg tatttaattg aagattgtgc
    62221 tcaatctcat ggagctaaat atgatggaaa aatgacagga acatttggtg atataggttg
    62281 ttttagtttt tatccaagta aaaatttggg tgcatttgga gatgcaggag ctatagtaac
    62341 tagtaatgag gaaatagctg ataaagtgaa aattcttaga aactatggct cagaaaagcg
    62401 atattataat aaatttgtcg gatataattc aagattggac gaaattcagg caggtttatt
    62461 aagagtaaaa cttaaatatc taaatgaaat tgaaattgaa aaatcaaaaa tcgtagatag
    62521 atatcttaat gaaattaata atcccaaagt catacttcca aagatagaaa ataattgtac
    62581 tcatgtatgg catttatttg ttttacaagt tgaaaaacga gatacattta tggaatactt
    62641 aaagaaaaat ggtatagagt ctataataca ttatccgata ccaccacatc tatctgaagc
    62701 atatgcttat ttgggatata aaaaggggag ttttaatata gctgagaact attcagataa
    62761 agttgttagt atacctttat ttatcggtat ggaagatgaa gaagttagtt atataattaa
    62821 aattataaat gaatattaaa ctactcataa taatatcaaa aataaatttt ttatatatca
    62881 tttatttaag tttcgttaat tgacattttt aaatattgat atattgtaca aataaaattg
    62941 aaatatatgg aaaaat
//
LOCUS       DAENCV010000005        61848 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAENCV010000005 DAENCV010000000
VERSION     DAENCV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.005"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            230..346
                     /locus_tag="KQD82_000265"
     CDS             230..346
                     /locus_tag="KQD82_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG4378770.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            510..1577
                     /locus_tag="KQD82_000266"
     CDS             510..1577
                     /locus_tag="KQD82_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4378771.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEAAKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1769..2299
                     /gene="rnmV"
                     /locus_tag="KQD82_000267"
     CDS             1769..2299
                     /gene="rnmV"
                     /locus_tag="KQD82_000267"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG4378772.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRYEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2470..3339
                     /gene="rsmA"
                     /locus_tag="KQD82_000268"
     CDS             2470..3339
                     /gene="rsmA"
                     /locus_tag="KQD82_000268"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG4378773.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3405..4667
                     /locus_tag="KQD82_000269"
     CDS             3405..4667
                     /locus_tag="KQD82_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378774.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4818..5933
                     /locus_tag="KQD82_000270"
     CDS             4818..5933
                     /locus_tag="KQD82_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4378775.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6119..7015
                     /locus_tag="KQD82_000271"
     CDS             6119..7015
                     /locus_tag="KQD82_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG4378776.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7085..7882
                     /locus_tag="KQD82_000272"
     CDS             7085..7882
                     /locus_tag="KQD82_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378777.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7937..9289)
                     /locus_tag="KQD82_000273"
     CDS             complement(7937..9289)
                     /locus_tag="KQD82_000273"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG4378778.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9508..10386
                     /gene="purR"
                     /locus_tag="KQD82_000274"
     CDS             9508..10386
                     /gene="purR"
                     /locus_tag="KQD82_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG4378779.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10542..10820
                     /gene="spoVG"
                     /locus_tag="KQD82_000275"
     CDS             10542..10820
                     /gene="spoVG"
                     /locus_tag="KQD82_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG4378780.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11033..12412
                     /gene="glmU"
                     /locus_tag="KQD82_000276"
     CDS             11033..12412
                     /gene="glmU"
                     /locus_tag="KQD82_000276"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG4378781.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12507..13457
                     /locus_tag="KQD82_000277"
     CDS             12507..13457
                     /locus_tag="KQD82_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG4378782.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14301..14386
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14548..15069
                     /locus_tag="KQD82_000278"
     CDS             14548..15069
                     /locus_tag="KQD82_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG4378783.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGEYKLIKVG
                     SKLVLQIGTNTEGVTLTEAQSAKLLSDIGEKKIYTSATTNSLGDPLTSNTKIDNKVLY
                     IVLIDNTVMDTTK"
     gene            15221..16897
                     /locus_tag="KQD82_000279"
     CDS             15221..16897
                     /locus_tag="KQD82_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG4378784.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16909..18117
                     /locus_tag="KQD82_000280"
     CDS             16909..18117
                     /locus_tag="KQD82_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG4378785.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18132..19844
                     /locus_tag="KQD82_000281"
     CDS             18132..19844
                     /locus_tag="KQD82_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG4378786.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19837..20745
                     /gene="pilO"
                     /locus_tag="KQD82_000282"
     CDS             19837..20745
                     /gene="pilO"
                     /locus_tag="KQD82_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG4378787.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20772..21338
                     /locus_tag="KQD82_000283"
     CDS             20772..21338
                     /locus_tag="KQD82_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG4378788.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21375..21902
                     /locus_tag="KQD82_000284"
     CDS             21375..21902
                     /locus_tag="KQD82_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG4378789.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21923..23461
                     /locus_tag="KQD82_000285"
     CDS             21923..23461
                     /locus_tag="KQD82_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378790.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23561..24643
                     /locus_tag="KQD82_000286"
     CDS             23561..24643
                     /locus_tag="KQD82_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG4378791.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24624..25406
                     /locus_tag="KQD82_000287"
     CDS             24624..25406
                     /locus_tag="KQD82_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG4378792.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            25508..26158
                     /locus_tag="KQD82_000288"
     CDS             25508..26158
                     /locus_tag="KQD82_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG4378793.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26172..26732
                     /locus_tag="KQD82_000289"
     CDS             26172..26732
                     /locus_tag="KQD82_000289"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG4378794.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26749..30135
                     /gene="mfd"
                     /locus_tag="KQD82_000290"
     CDS             26749..30135
                     /gene="mfd"
                     /locus_tag="KQD82_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG4378795.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30166..31161
                     /locus_tag="KQD82_000291"
     CDS             30166..31161
                     /locus_tag="KQD82_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG4378796.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31285..31824
                     /gene="spoVT"
                     /locus_tag="KQD82_000292"
     CDS             31285..31824
                     /gene="spoVT"
                     /locus_tag="KQD82_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG4378797.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31944..33557
                     /locus_tag="KQD82_000293"
     CDS             31944..33557
                     /locus_tag="KQD82_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG4378798.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33664..35112
                     /gene="mazG"
                     /locus_tag="KQD82_000294"
     CDS             33664..35112
                     /gene="mazG"
                     /locus_tag="KQD82_000294"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG4378799.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35201..35479
                     /locus_tag="KQD82_000295"
     CDS             35201..35479
                     /locus_tag="KQD82_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG4378800.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35551..35793
                     /locus_tag="KQD82_000296"
     CDS             35551..35793
                     /locus_tag="KQD82_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG4378801.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35855..36109
                     /locus_tag="KQD82_000297"
     CDS             35855..36109
                     /locus_tag="KQD82_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG4378802.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36110..36643
                     /locus_tag="KQD82_000298"
     CDS             36110..36643
                     /locus_tag="KQD82_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378803.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36649..36933
                     /locus_tag="KQD82_000299"
     CDS             36649..36933
                     /locus_tag="KQD82_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG4378804.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37046..37966
                     /locus_tag="KQD82_000300"
     CDS             37046..37966
                     /locus_tag="KQD82_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG4378805.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38056..40428
                     /gene="spoIIE"
                     /locus_tag="KQD82_000301"
     CDS             38056..40428
                     /gene="spoIIE"
                     /locus_tag="KQD82_000301"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG4378806.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40779..42671
                     /gene="pepF"
                     /locus_tag="KQD82_000302"
     CDS             40779..42671
                     /gene="pepF"
                     /locus_tag="KQD82_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG4378807.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42785..43750
                     /gene="galU"
                     /locus_tag="KQD82_000303"
     CDS             42785..43750
                     /gene="galU"
                     /locus_tag="KQD82_000303"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG4378808.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNESRELEIQK"
     gene            44250..45854
                     /gene="rho"
                     /locus_tag="KQD82_000304"
     CDS             44250..45854
                     /gene="rho"
                     /locus_tag="KQD82_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG4378809.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46007..46207
                     /gene="rpmE"
                     /locus_tag="KQD82_000305"
     CDS             46007..46207
                     /gene="rpmE"
                     /locus_tag="KQD82_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG4378810.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46352..47245
                     /locus_tag="KQD82_000306"
     CDS             46352..47245
                     /locus_tag="KQD82_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG4378811.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47263..48111
                     /gene="prmC"
                     /locus_tag="KQD82_000307"
     CDS             47263..48111
                     /gene="prmC"
                     /locus_tag="KQD82_000307"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG4378812.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48155..49219
                     /gene="prfA"
                     /locus_tag="KQD82_000308"
     CDS             48155..49219
                     /gene="prfA"
                     /locus_tag="KQD82_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG4378813.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49348..50028
                     /locus_tag="KQD82_000309"
     CDS             49348..50028
                     /locus_tag="KQD82_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG4378814.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50057..51097
                     /locus_tag="KQD82_000310"
     CDS             50057..51097
                     /locus_tag="KQD82_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG4378815.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51114..51563
                     /locus_tag="KQD82_000311"
     CDS             51114..51563
                     /locus_tag="KQD82_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG4378816.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51580..52032
                     /gene="rpiB"
                     /locus_tag="KQD82_000312"
     CDS             51580..52032
                     /gene="rpiB"
                     /locus_tag="KQD82_000312"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG4378817.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52068..52697
                     /gene="upp"
                     /locus_tag="KQD82_000313"
     CDS             52068..52697
                     /gene="upp"
                     /locus_tag="KQD82_000313"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG4378818.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52998..53813
                     /locus_tag="KQD82_000314"
     CDS             52998..53813
                     /locus_tag="KQD82_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG4378819.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPFTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54039..54476
                     /locus_tag="KQD82_000315"
     CDS             54039..54476
                     /locus_tag="KQD82_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG4378820.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54661..54888
                     /locus_tag="KQD82_000316"
     CDS             54661..54888
                     /locus_tag="KQD82_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG4378821.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54893..55279
                     /locus_tag="KQD82_000317"
     CDS             54893..55279
                     /locus_tag="KQD82_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG4378822.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55282..55986
                     /gene="atpB"
                     /locus_tag="KQD82_000318"
     CDS             55282..55986
                     /gene="atpB"
                     /locus_tag="KQD82_000318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG4378823.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56055..56315
                     /gene="atpE"
                     /locus_tag="KQD82_000319"
     CDS             56055..56315
                     /gene="atpE"
                     /locus_tag="KQD82_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG4378824.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56438..56935
                     /gene="atpF"
                     /locus_tag="KQD82_000320"
     CDS             56438..56935
                     /gene="atpF"
                     /locus_tag="KQD82_000320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG4378825.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56932..57477
                     /locus_tag="KQD82_000321"
     CDS             56932..57477
                     /locus_tag="KQD82_000321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG4378826.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57494..58996
                     /locus_tag="KQD82_000322"
     CDS             57494..58996
                     /locus_tag="KQD82_000322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG4378827.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59024..59902
                     /gene="atpG"
                     /locus_tag="KQD82_000323"
     CDS             59024..59902
                     /gene="atpG"
                     /locus_tag="KQD82_000323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG4378828.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59915..61309
                     /gene="atpD"
                     /locus_tag="KQD82_000324"
     CDS             59915..61309
                     /gene="atpD"
                     /locus_tag="KQD82_000324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG4378829.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61312..61572
                     /gene="atpC"
                     /locus_tag="KQD82_000325"
     CDS             61312..61572
                     /gene="atpC"
                     /locus_tag="KQD82_000325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG4378830.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25028 a         6596 c        11471 g        18753 t
ORIGIN      
        1 attaaataat tataaactaa agtaaataaa atattgagcg attattaaaa agatatatat
       61 atataattta atttgagcag tttgttatat attataaaaa agagaaaaat aaaaggtaaa
      121 ttgtttctgg ataacttaaa attggaagta agaatattta actagagaag aattatttaa
      181 gctcaggtta gctctaggag tagctgattt tataggagca atagatttta tggatgatga
      241 tataaacatg atagcaatta gaccagaaga tataagggta gaatttaata gagatttcaa
      301 agaaattata gacctcaaac tcagaccgta tcgatataaa aactaaagct agatgatata
      361 gtgttgtata taggaccatc agctgttatg gcagtattag tgcttttaac aaatataata
      421 gtaaaacttt tgtatgaatt gataataaaa aaattaaaaa ccaagacata aaaatggcaa
      481 caaaaagaag ttgaataagg gaggtaaaaa tgaaatttaa gaagttagtc tctttgggaa
      541 tattatcaac acttttattg agtgtaataa ctggatgtag taatgtttca tcaggttcaa
      601 acttaaagtc taataataag acaaaaggag agttaacagt ttatacagca attgaggaag
      661 atagtataga tacatactta gctacattta aagagaaata tccagatatt aaattgaatg
      721 tacttagatt atcagtagga ggtataacag caagattatt agcagaaaaa gataatccta
      781 aagcagatgt gttatggggt gttgcagcta caagtttgat aattttagat gacaaagaaa
      841 tgctagagcc atacgctccg aaaggctttg ataaggtaaa tcctgcattt aaagatgata
      901 agaaagttcc ttcttgggtt ggaatagatg cttggatgac tggtataaca gtaaatacta
      961 aagaattaaa agctaaaaat atatcagctc ctagttctta tgaagattta ataaagccag
     1021 aatataaaga tcttatatct atgcctagtc cagcttcttc aggaacagga tatttaacag
     1081 tatcagaact catacaaatt atgggtgaag aaaaagcatg gcagtacatg gataaattac
     1141 ataaaaatat tggtgtatac actcagtctg gttcagcgcc agctatatta gcagcttcag
     1201 gagaatatcc cattggtata tcttttggaa atagaggtat aaagttaaaa ggtgaaggtt
     1261 atccagtaga agtagtattt ccaaaagaag gttctggatg ggagatagaa tcaaatgcac
     1321 ttgtgaaaaa agataatata aaagaagcag caaaagtatt tttagattgg gctataagta
     1381 aagatgcaat gaatgagtat tctaagaatt ttgcagttac tacaattgac acgggaaatc
     1441 cggtgccaga aggatttcca aagaatccat tagaacagat gatagataat gatttaaaat
     1501 cagcagcaag aaatagagag gctatactaa ataaatggat agaaaaatat gatggaaaga
     1561 cagaaaaaga gagctaagcc ttacaataaa atttagtaca aaaagcgcct agaatataag
     1621 ttttatatct aggctctttt gatatattta tttaaaatgc atatttctat ataaaaataa
     1681 atagtttagt accttgatta aattaaataa aaaccttaaa ataaagagat gtaaagaaaa
     1741 actaaaaacg aataaaaggt gaatttatat gataaaagaa ataatagtag tagagggtag
     1801 agatgacgtt acagcagtta agagagcaat agatgcagaa cttataacta caggtggatt
     1861 tggatttcca aaaggtgtaa tggagcgtat aaaagctgct aatgaaagac gtggtgttat
     1921 aatatttaca gacccagatt ttgcaggtga aaaaataaga aaaaaaatag catcagaagt
     1981 tccaggatgt aaacatgcat ttctaccgag agaggaagct aaaaaggatg gagatatagg
     2041 tattgagaat gctactcctc aaagtataat agcagctctg aataaagtta gaactgaaaa
     2101 tacagaaaaa agatatgaat ttaagcaagt tgacttaatt agaaatggtc ttataggtaa
     2161 tgaagaagca tcacttagaa gagatgcact tggaaaaata cttggaattg gatatggaaa
     2221 tgcaaaacaa tttttaaata gattgaataa ttatggagtt gaaagagaag aatttataga
     2281 tgcattaaaa caaatctaaa cgtaatgttt aatgtatgaa aaatatgtat ataaaataaa
     2341 ttgtggaatt acggttaatt taagataaat tatttataat atatacctta ttttaaatca
     2401 atgtaaatac atataaataa gtataattaa taaaattaaa tatcaaaata agttaaaaag
     2461 gagtaaataa tggatagact atcttcacat aatgcaacaa aggaagtagt acaaaaatat
     2521 aattttaaat tttcaaagtc tttaggtcaa aatttcttga tagatagtaa tataattgat
     2581 aaaatcttat ctggagctag aatcacaaga ggagataata tcatagaagt aggacctggt
     2641 ataggaactc ttactcgtga gatgggtaaa atagcagaaa aagtagtggc tatagaaatc
     2701 gatagaaatc taattcctat attgaaagat accttgtcag atttagataa tacagaggtt
     2761 gtaaatcaag atatactaaa agtagacata caggaattag taaaagataa attaaatggt
     2821 gggccagtta agcttgtagc aaatcttcca tattatataa ctactccaat agttatgaaa
     2881 tttttagagg aagacatacc tgtaacagac atagtagtca tggttcaaaa agaagttgca
     2941 gatagaatga atgctattcc gggtactaaa gattatgggg cattatctat agctgttcaa
     3001 tactattgtg atacagagat tgtggcaaaa gctccaagac atatgtttat accacaacca
     3061 aatgtagatt ccacagttat aggtttacat gtaagagata aaagaaaata tgatgttcat
     3121 aatgaagaca tattttttaa aacagtaaaa gcatcctttg gacaaagaag aaaaactctt
     3181 cttaattcgt tgggaggtct tggttttctt aataaagatg aaataagaga agtattaaaa
     3241 gaagctaata tagacgaaaa aagaaggggt gaaactctta gtatagaaga gttttcagtt
     3301 ctttcaaata taataaatac aaaagtatct tctaaataga aggtactttt tttaattttg
     3361 ttaacatata ttgtttagag aaactgtgtt aaggggggat tgcagtgggt tctaaaagaa
     3421 agtttaaaag agactatata atactagaag ctaaagacat aaactttaga tacaaagaac
     3481 gagtactgcc aaaagctttt gcaaaagttg aagtaaatga tgaaaaagca attattgcat
     3541 tatatgtaga aaatctaaaa tatgttaaag atgggtataa agcagtagca ataaaagatg
     3601 accatggatt aatagactta ggaaatataa ttttaaatga acaaggaaga ggtgaatttg
     3661 ttttagattt agaagattat gatgatgaga taaaaggtat tgcattattg tatgaaagaa
     3721 atataccttt aattggattt aaaggaaata agcttgaaaa ttatgaggac atattatttc
     3781 aagaggtaga ggaagaagaa tatgaagaat atgaagagtt ggaagaagaa gacgaagaga
     3841 tagaggataa tgaggaaagt gatagagacg aaggtagtga agaagaatat attatagaag
     3901 aatatgatga agatgagtat gaagactatt gggaagatga tgaagatatt gaagaaggag
     3961 agttaaaagc aagaacggaa gtagaagatt ctagtgatga ggaaatagaa gagttcaata
     4021 attataagga aatagaaaat tataataggg ttaaagtaca ggaagaatat atagacatag
     4081 aatatgatga agaagaagag gaagaagaag tttttgaagt aaatagagaa gaagataggt
     4141 ttgtttcctc aaaacctact aaatcaaaga aagttcaaaa agatcagaat aatattgaaa
     4201 aaataaaaag agctaatgga gtaagtcaag gagagagtgg aaataaacaa accagtacat
     4261 tacttatgcc aagacaaata aaaaaaggtt tgaaattatt taaagaagta aaaccttttg
     4321 taacagatta tatagataag acaagatggt ggaaaataga gataaatcct acaacactgt
     4381 gtggatatac aatgccttat ttaggatatt taaacgtatt gaattacact atgtatagtg
     4441 atgcagtttt acattcatat aaatacagac actatttatt tggagttcag tatgatgaat
     4501 ataataagag aaaacattat atttatgcta tcccaggaaa tagaaatgaa cagccagaca
     4561 atggaagcac aggattttta agatatcaag cttgtgataa tagaacaaat agtttaggat
     4621 attggctatg ttttatagat tctagacaaa gaaatatagt taggtaaaaa taataaaaat
     4681 gaaaaaattt agactcaatt cttattttac tgaaaaaatt aatgaaatgt agtacaaaac
     4741 tttaaaaact attgataata tttaaatatt acactacaat atatcttgaa atacctatta
     4801 tattatggag gaaaattatg ataaacgagc aaagaatttt aaatgagttt cttgaattag
     4861 tacagataga tagtttatct ttaaaagagg ggaatgttgc taaagtatta gttaaaaaat
     4921 tagaagaaat aggttgttca gtagtaatag acaatgctgg tgaaaaagca aatggagaaa
     4981 ctggtaatat aatagctaca ttaaaaggaa ataaagaagg aaagaaaata ttatttagtt
     5041 cacatatgga tacagtaact ccaggtatag gggtaaaacc tatagtggat gaagcaaatg
     5101 gaataataaa aagtgatggt actacaatat taggttctga tgataaagca ggtatagcag
     5161 ctatattaga aggcttaaga tatataaatg aaaataatat agaacatact gatatacaag
     5221 ttgtattctc tatatgtgag gaatgtggat tagttggagc taaaaattta gactatggaa
     5281 aaatagattc tgaatatgca tttatattag atagtggtgg ttctccaggt gaaataatag
     5341 taaaagctcc tgcacaagat gtaataaatg ttaagatatt aggaaagact gctcatgcag
     5401 gtcttgaacc tgaagctgga ataagtgcta taatggtagc tgccagagca atagaaaata
     5461 tgaatttact tagaatagac gaagaaacaa ctgctaacat aggaattata aacggtggaa
     5521 cagctacaaa tatagtaaca ggagaagtta atataattgc agaagccaga agtctaaaag
     5581 aaaacaaatt agatgttcaa actaaacaca tggtagagac ttttgaaaaa gctgcaaaag
     5641 attttggagc tcaaattgag atagatgtca atagagcata cacaccaata gatgttagtg
     5701 aggattctga aataataaaa cttgcaaaga aggctttttc aaacttaggt atagaaggtc
     5761 atactgaatc tacaggagga ggtagtgata caaatatatt aagtaagaat ggtatcgaag
     5821 ctataacttt aggaattggt atgaaaaatg ctcatacact aagtgaacat atagctataa
     5881 aagacttata tgattctgct aaaatggtag tggagataat aaaggaagca taaatttaaa
     5941 taatagtgta taatatatat atcgcctagc ttaagctagg tgatttttca ttttatagaa
     6001 gattgataat ctctattgaa agcaaagagt atacatttgc atataagaga gtgtttaatt
     6061 tatgttcaaa tgtaggttct taataatatt tagattgttt cataagggag gaataaatat
     6121 ggaaacaagg tatgaagaag caaataaaat aacaatacaa tcaatacttt ggaatatagt
     6181 attaacaata attaaggtta tagctggtgt tattggtaat tctagcgcta tgattgcgga
     6241 tggattacat tcagcatcag atattataag ttctatagga gtactaatag gcaattatgt
     6301 atcttctaga cctggagata gagagcataa ttatggtcat gagaaagcag agacattagt
     6361 atcatttgtg ttatcaatat tgcttatttt cgtttctata acaataggta tagaggcaat
     6421 taaatcttta tttaacttag atgcattaag tgttccatca atattaccat tagtagtatc
     6481 agttatttca atcctgataa aagagtatca atatagaata acaataaaag ttgctaaaaa
     6541 gataaattca ccagccctaa aagctgatgc ttggcatcat aggtcagatg ctttatcttc
     6601 agtagctgct tttataggta taggtggttc tattttaggg tttaaaccat tagacccaat
     6661 tgcgtcagtt gtagtggcta tatttgtagc taaagtagga attagtatac ttataagttc
     6721 tgttaatgaa ttgatggatg tttcagttga tgaagaagag ataaaagaat taaaatttat
     6781 tgtagcagat acagaaggtg ttaaaaatct tggtgatata aagactagaa aacatggagc
     6841 tatggcatat gtagatttga ctatatgtgt ggatgaaaat ttgacagtta agcaaggtca
     6901 tgatattgct accaagcttg aaaaacatat aataaaacat atggaatttg taaaaggcat
     6961 aacagttcac gttgaaccat gtactaattg ccaaggaaat aagtgtaata attaattaaa
     7021 acttaaaaaa aagtataaaa agttatataa ttgttttatg attataaact ctaggggtgg
     7081 ttaaatgagc aagaaaagaa aactaaagaa aaaagctgca atattaatag gagtagttgt
     7141 tttctttata atatacttta tatcctttag aataggaata aacatggaaa aggataaaag
     7201 tgaagtgcca aagaaggtag aaaaagaaga agaagataat aggtctttaa ggactcaaat
     7261 aaaagagaag aagaaggttt atatatcaga taaaaatata gaaaacataa aaattgaaga
     7321 agatatgtgg gatgaaataa agttattttt ctcagatttt aagaaagtaa gaaatactac
     7381 ttcttcttac gaagcagttt atgaaggtta ttctgatgat ggagttaaat tttctactga
     7441 ccttaacttt tttagagtat atactgtaaa taaagaagaa tattataaag taccagttag
     7501 ttctaaggat gcttttaaga aacttttaga tgagagtata tatacttcgt ttgacctttt
     7561 aagtcattat aaagattgga aagaggttac tataactcat ggaaaagaaa ttaaaaaagt
     7621 tcatagatgg aaatttgatg atttagcaca taagatgatt tcaaagaggg ttgtaggaaa
     7681 agttcaacct gagaaaagta aggaaagaag tgactataat tttactataa atataaaagg
     7741 tgataatttt gatgtaaaag ttgatacaat gggaaaagat tatgtaaaag ttacttctaa
     7801 gaaaaatgta tcttactatg aggttcatac aagtttattt gattacctta aaaatgagat
     7861 atttgaaatt ggaaagaaat aagactttaa gaaggctatc aaattagata tttgatagcc
     7921 taaattattt tatatattat ttttcaacca acaagtctgc aaccttataa attggaccag
     7981 caccagcagt tattactaaa tcattatcct taacattttc acgtaagtat tttactatat
     8041 cttcaaattc ttttatatag atggcatcta cattgttttg atataatttc tcaaccaagt
     8101 ccttagaatg tatatctcct gggtcttttt ccctagcagc atatatatct gttatgataa
     8161 ctttatcagc agcatagaat gcctctgaaa attcacctaa aagagatttt gttctagtat
     8221 aagtatgtgg ctggaagata caccataaag ttgatttttt taattttttg gcagcagcta
     8281 aagttgcttt taactcagtt ggatgatgtg cataatcatc tactactaaa gcattcttat
     8341 agtacccttt agtttcaaat cttcttccga cacctttata aatttttata ttttttctta
     8401 tagtctctaa gtcaatacca gaaacaagag ctgccattat ggcagaagaa gcattgtata
     8461 tgttatgaag accatataca gaaagttcaa attctcctaa atctcttcct tcatattcta
     8521 ttctaaatat tccatgacca ctattgtcaa agtgaatatc ttttattaca gcatcattgt
     8581 cagaatctct gccgtatttt ataatagtag ctttaacatc atacaatatg tcatcaatat
     8641 tttcatcatc gccatttatt atgaaatagc cttctggggg taataatttt ccaaatttgt
     8701 taaaagaagc cttgatttca tctattccag agaaataatc aagatggtct tcttcaacat
     8761 ttaaaactat agatatcttt gggttaaaat taaggaaact atctacatat tcacatgctt
     8821 cagttataaa gtggttggaa ttacctatct taacattacc acctatcata cttaagttac
     8881 caccaactag tatagttggg tctaagtctg catattcaaa gattgtagat aacatagatg
     8941 ttgtagaagt ttttccatgt gttccagaaa ctgctataga atttttatat tccctcatga
     9001 tttggcctaa aaaagttgct ctgttcatta ccaatttatt tttttcttta gcagccacaa
     9061 gttcttcatt gtctggatga actgctgctg tataaacaac catatttaca tcatctgata
     9121 tattatcttt tttctgacca atataaatag ttgccccttg gtccctaagt ttgtctaata
     9181 ggtatgattc gtttgaatcc gaacctgaaa cttgataacc tttattgata catatttcag
     9241 ctaatgcact catgctgata ccgccaattc ctataaagtg tatattcata ataaaaccac
     9301 ctttcttttt aattaagtcc tacaatatga actatttctg tggtataata atataatgtt
     9361 caatatttta taattaatat gcatataata ttatatgtat tttatttttg aatataataa
     9421 aacataagta atattatacc acaataagaa ttgttatgca ttagttagtt taacgttata
     9481 taaaggtact tgtaggagga aataataatg aaatttaaaa gaacagagag aattggagca
     9541 atagttaaaa tactgtctga taatccaaat aaaatatata ctttaagtta ttttacaaat
     9601 caatttaatg cagcaaaatc aacaataagt gaagatttat tagttgtaaa aaatgtattt
     9661 gagaagctac atttaggtaa agttataact atttcaggag cagcaggagg agtaaaatat
     9721 atacctaaaa cttctatagc agaaaatgca gagttcttaa tggaattatg cgagagaata
     9781 tgtgacaagt ctagaatact atctggtggt tttttatact tgattgacct aatttacgac
     9841 cctacaatag ctgctaagat aggaaagata ttcgcatcaa atattgagta tgtagatgca
     9901 gattatgttg tgacaatgga aacaaaaggt atacctatgg cacttatgac tgcaaaagct
     9961 atgaatttac ctctagttat aataagaaaa gatataaagg tatcagaagg tcctacactt
    10021 agcatgacat atgtgagtgg aaacagttct aaagttgaaa gtatgagttt gccaagaaaa
    10081 gctcttaaac cagatagtaa agttataatt atagatgatt tcatgagggg tggaggtact
    10141 ataaaaggaa tggtagacct tatgaatgaa tttggtgcag aggttatagg tactggagta
    10201 ttcatatcta caacgaatcc ttcagaaaaa atggtaaaag attatatttc attgattcaa
    10261 ttggatgtta atggagataa aatagtagta gaaccaaact taaagacttt taaagatgaa
    10321 tatagaaatg aagatttaga cgaagaagaa gattcagaat ttgaatttga gatagatgaa
    10381 gattaataaa aaaaatattt agaaaaatat gtaaaaataa aaagaggata tccctagttg
    10441 ttcatagaat tatttaaaaa acagtttata ttaatttatt ccagcgataa taattttaat
    10501 ataaattaat ttctgtcaaa atcaaagggg gatattttgg tatgaagata actgacgtaa
    10561 gagtaagaaa attaacagaa gaaggtaaga tgaaatgtat agtttcaata actttcgata
    10621 atttgtttgt agtgcatgat ataaaagtta tagaaggcca taatgggcta tttatagcaa
    10681 tgcctagcag aaaagtaggc gaaggaaatt tcagagatat agctcatcca ataaatgcag
    10741 aaatgagaca agttttagaa gatgcagttt tacaagcgta tcatgaagca ttagttcaat
    10801 gggaagtagc tgctgaataa taataaatat ataaaatgga gagagcccga acatttaggc
    10861 tctttttaaa tgtagaattt ctattaaaat atgtactttt tagtaaaaaa gtgctatatt
    10921 attagtgata atgtatatgt tgtatacaaa ttgttagata aatgtattat aatgtatatt
    10981 gattaagaga ataatacgaa taaaattaaa ttagattgaa tggagtgtat caatgaactt
    11041 taaagctata atccttgctg ctggcaaagg aacaagaatg aagtctaaat acccaaaagt
    11101 tatacataaa gtatgtggaa aagaaatggt taaccatatt atagatgttt caaaaaaatc
    11161 aggggtaaaa gatactgtcg tgattttagg acatgaagct gatgtagtta aagaaaagct
    11221 tgcagaagaa attataatag ccatgcaaac agaacaactt gggacaggac atgctgtaaa
    11281 gatggctaaa gaatatatta atgatgaaga tacaatagtt gtactttgtg gagatactcc
    11341 acttataaaa gaagagacac taaagagact atttgaatat catatagaaa ataaatatca
    11401 tgctacagtt cttacaacaa gagttggaaa tccaacaggt tatggtagaa taataagaga
    11461 taagaagggt gaccttctta aaatagtgga acaaaaagat gcaaattcag aagaaaagat
    11521 gataagtgag ataaactctg gaatttattg ttttaatgga aagagtctta gagaagcact
    11581 agatttgcta aataataaca attcacaagg tgaatattat ttaactgata ctgctaaaat
    11641 aatgagagat aaaggattaa aagttggtgc atttgcaggt tcaactattg aagaacttat
    11701 gggcgttaac tcaagagtag aattatcaaa ggcagaagaa attatgagac gaagaataaa
    11761 tgaatctcat atggtaaatg gagtaactat aatagacact aactctacct atatagaatc
    11821 tgatgttatg ataggcaatg atactataat atatccaggt gttatgttac aaggaaaaac
    11881 aaggataggt tcagattgta taattggaat gaattcatct ataacaaatt cagaaatagg
    11941 tgatggtaca gaaataaaaa attcaactat aattgatagt aaggttggag aaaattctac
    12001 tgttgggcca tatgcatact taagaccaaa gagtgactta ggaaataatg taaaaatagg
    12061 cgattttgta gaagtaaaaa atgctattat agaagatggc tcaaaagcat ctcacctttc
    12121 ttatatagga gatgcacatg tcggtaaaaa tgtaaatatt ggatgtggag tagtatttgt
    12181 aaactatgat ggtaaaaata aatttaaatc aatagtaaaa gacaatgcgt ttataggttc
    12241 aaattctaat ttagttgcac cagtagttgt agaagaaaaa ggatatatag caacaggttc
    12301 aacaataaca catgatgtcc cagatggtgc tttggcaata gcaagagaaa gacaagttat
    12361 caaagaagga tgggtagaaa aaaagaatca aaaagacgac caaagtaagt aattatgtag
    12421 tttattattt tggctaaaaa atttagctcg aaattattat aaattatatt cattagtatt
    12481 ttaaaatttt caggagggtt acataaatga atactagcgg aagcgagatt aaaattattg
    12541 caggtaattc atcgaaagag ttagcacaaa aaatagctga ctatataggt gtatcagtac
    12601 tagattgtga agtaggtaca tttagtgatg gtgaaatatg tgtaaacatg aatgagacag
    12661 taagaggttg tgatgtgttt gtagtgcaat ctactaacag cccagtaaat gataacttaa
    12721 tggagttatt aatcttaatt gatgcgttaa agagagcatc agcaggtaga ataacagcag
    12781 ttattcctta ctatggatat gcaagacaag acagaaaggc taaggcaaga gatccaatca
    12841 ctgctaaatt agttgcaaat ttaataactg ctgctggagc agatagagta ttaacaatgg
    12901 atttacatgc agctcaaata caaggatatt ttgatatacc attagaccac ttactaggtg
    12961 gaacaatatt agcaaattat tttaatgaaa agaaaataga agatttagta gtagtatctc
    13021 ctgacttagg aagtgttact agatcaagaa aatttgctaa tactttaaat ggagaagttc
    13081 caatagctat aatagataaa agaagaccga aagctaatgt atgcgaagtt atgaacttaa
    13141 taggtgatgt taaaggtaaa aatgttatac tattagacga tatgatagat acagcaggaa
    13201 caatagttaa tgctgcaaat gcacttaaag agtttggagc aaaagatgtt tatgcttgtt
    13261 gtactcatgg tgtgttatca ggtccagcta ttgaaagaat tgctaattca gaaataagtg
    13321 agttaatagt tcttgatact atacaacttc ctgaagagaa aagaatagat aagataaaga
    13381 taaaaacagt agctccttta tttggagatg ctataagaat gatattctct aatgaatcag
    13441 taagtaaatt attctaattt attgtattta tgagagttgt ttaaaaggat ataaaccttt
    13501 tgagcaactc ttttttgatt ttataattat ggaatgttat ttttttcttg ttagaaaggt
    13561 atatattatt gtaaaaagag attatagttt aaaattatgt atatttaagt aaaataaact
    13621 taataaaggg catattattc aaaatattat gaatataaaa aatattacaa aaaagtattg
    13681 aaaaaattca gattatgtag tattatgtaa atataaataa agaaagaaag aggtgtttcc
    13741 aatgggaagg ttagaagatg aacccctaat aattaaatga ttcagtcaca atctaaagtg
    13801 aatataaaaa tctattgaaa gtttatttat aagaattgaa aatagtatct attctatctt
    13861 tagtgtttgt gagagggttc tggattatat aagattactg taaagaagta attttgcaca
    13921 ataagcatat ctgtaaatga agagtttcgt ttatataatg attgtggctg aatcataact
    13981 ataatagata gaatgtttat aatagatgat tttataaaat actatattgt aatagctttt
    14041 agctttcaaa taaatattag caaaggatgg aaatgacaat gataaaacta aatgataatt
    14101 tataaatatc tccttacgat atataataat tgtagggaga tgtttctttt ttttgtagag
    14161 tataattttt ctagaaattc tgtttcaata tgtaaaaagt tggtatatat aaatagtata
    14221 aacagtgttg ctaaaatatg gtagatatta tataatataa atggatatgt atatacatta
    14281 tgagttatat aatttgaatt taataaaata gaaacgttga tttatgttct gtaatgtggg
    14341 caccttggag catattgagt tagtggtgca accggctatg aaattgtatt tatttataaa
    14401 tactattatt ttcatagctt tttttattta aaaaatggtt atatgttaga aataaatttg
    14461 gttagtacgt ttaaataatt ttacttagat ttttaaccag atatattata agtaagtatt
    14521 aattaaaaaa tttaaggggg aataaaaatg aagttaaaaa agaataaaaa aggtttcact
    14581 ttagtggaat tattggtagt aattgcaatt ataggtatat tagcagtagt ggcagttcca
    14641 gctttattta gtaatataaa caaggctaag gtagcaagtg ttgagtctga ttatagttca
    14701 gttaagagtg ctgcattatc ttattattca gatactaata agataccagt tacaccagat
    14761 ggtcaaactg gtttaagtgt tttagaaact tatatggagt ctcttcctga taaagctgat
    14821 ataggtggag aatataaatt gattaaagtt ggtagtaaat tggtattaca gataggtaca
    14881 aatactgagg gagttacctt aacagaagca caatcagcaa aattattgag tgatataggt
    14941 gaaaaaaaaa tatatacaag cgctacaaca aatagtttgg gagatccatt aacaagtaat
    15001 acaaaaatag ataataaagt tctatatata gtacttatag ataatactgt gatggacaca
    15061 acaaaatagt taaaacaata tttttataaa gaatattgta aatttaatga aaaatataat
    15121 ataaaagaca ggaagaaaat cttcctgttt ttttatacta ttttctttaa tgttcaatta
    15181 tgaatgatga tataattgag atagtaaagg agtgaaacct gtggctaaaa aagttagaat
    15241 aggagacaaa ctggtcgaaa aaggatatat aacagaagaa cagcttaaat gggcactatc
    15301 agaacaaaaa aacagtggaa aaagactagg agaatttcta gtacaagaag ggcttataga
    15361 tagtaactta ctaataagtg tattaaaaga actcttggat atagaaagta tatttttaga
    15421 agggacagaa atagacactt tagctacaaa aatggttcca gaaaatatat gcaaaagata
    15481 tactgtattt ccatttaaaa ttgatggaaa taaaatatgt ctagccatgt cagacccaca
    15541 agatagagaa gcagttcaag atgtaaggcg tatgtctggt aaagacgtag agatatttat
    15601 atcaagtaca gaagatataa acaaagcaat aggtcatgct tatgcacatt cagaaataaa
    15661 taaggcaatg actgagtaca acaaaaatag aactggtgga gtaagagaaa cagtaatact
    15721 tgaagaagat gtaaatgctg ctccaatagt acgattagta aataacatac tagaaaatgc
    15781 tgttagaatg gaagcaagtg atatacatat agaacaaagt gaaaattaca tgcgtgttag
    15841 gtttaggata gatggaatgt taagagaata tatgaggatg aactcagcac catataaggc
    15901 agtaatcagc cgtatcaaga taatgtcaga tataaatata tctgaaaaga gaataccaca
    15961 ggatggtaga atctatttaa aagtagataa taaacccata gattttaggg tttctacaat
    16021 gccaactaac agggatgaaa agatagctat gagagttctt gataagagta attttatggt
    16081 tagtaaagaa gtactaggaa tagacgaaca tggctctaag atatatgatg aattgataaa
    16141 cactccttat ggtcttatat tggttgttgg ccctactgga agtgggaaaa ccactacatt
    16201 gtattcaatg ttaaatcaac taaatacaga aaatagaaat ctattaacaa tagaagaccc
    16261 tatagagtat gaattacctg gtgtaaatca atctcaaata aatgaaaagg cagggcttac
    16321 tttcgccagc ggacttagag cttttatgcg tcaagaccca gatataataa tggttggaga
    16381 gataagagat actgagacag cagaaatagc aattagagct tctcttacag gacatttagt
    16441 tttaagtaca ttacatgcaa atacagcagt tggagcaatt tcacgtcttt tagatatgga
    16501 tgtggaaagt tttttaataa cttcatctgt tttaggtgta atatctcaaa gacttacacg
    16561 aaaaatatgt gagcattgta aggtttcata tgaagcagat attggagaaa aaaaggctct
    16621 tggtattgat gtaaatgaat cagtaactat ttatagagga aaaggttgtg aaaggtgtaa
    16681 taatactgga tataaaggac gtctaggtat atttgaaatg cttgaaatta ctccagaaat
    16741 aaaagagtta atagattcat ctgcaaatca aagagaaatt ttaaagatgg ctagaaaaca
    16801 aggtatggtt agcttaaaag aggatatagt taaaaaagtt ttaaatggaa agacaacagt
    16861 agaagaaatg attagaataa ttttgatgac tgattaaagg atgatattat gaaaagtttt
    16921 aaatacacag taataaaaga ggacggaaaa aagcaaactg atgtgattga agccaacgat
    16981 tttaatgaag ctagaaatct tcttagaaag agaaatctta gagttattga aatcaaagaa
    17041 agcaaaaata aaaatataag attatctagt agaaagagaa aaaaagactt gggagctgac
    17101 cagataagcc acttttgcag acagtttgct attatagtat cttctggtat aaatagtatt
    17161 tctggattag aaacattagc tagaaggtca accaacatta cattaagaga agaaattaat
    17221 cgtatagtgg cagaaataaa agtaggttct actattgcag attcaatgct ttcatcaaaa
    17281 tcaaagtttc caaagttact cggtgctatg gtagctacag gtgaagctac aggtaaatta
    17341 gatgaagtat taaaaagtat ggcttcattt tatgcaagtg aacacagggt taaacaaaag
    17401 cttagaaatg ctgcaacata tccattaata gttttgattt cttcatttat aatgattttt
    17461 atatttacaa catttatggt acctaagatg attaattcaa ttacaacagt aggagcaact
    17521 cttccactta taacaagaat tgttatgggg tttggtatgt ttatgaagaa gttttggata
    17581 atagtactct tagttattat attatttatc tatcagttta aaaaatatct aaaaacgtca
    17641 gttggaaggg ctcataaaga tagagttata aataagatac ctgttttagg aaaaggaata
    17701 aattgtatgg tagctacacg attttcaaga gcattgtatc tatttgtatc aacaggatac
    17761 ccattagttc aagggcttga ttatattata gatagtgtaa ataacaccat ggcagagaag
    17821 attttagcat cagctaaaga tggaataaca agaggagaag gcttagcaga aaatttagag
    17881 agatatacgt attttgactc tgtgttggta cagatgatag ctatagggga gcagacaggg
    17941 gaattagaga atataagtag acagatggca gaattttatg agtatgaatc cgagatttat
    18001 ttaaatagga tggcctcaat gatagagcct gttttaataa ttgcagttgg tattatagtt
    18061 gcgatactgg ttgtatcaat atttatgcca atgcttagta tatatgatgc catgtaggag
    18121 ggaaaggaca aatgaacaag gtttatgtat catattatgg cgattctatt tccatagttg
    18181 agggcagata taaacacgat aaggataagt tttatgtaaa aaattttaat gtgttttcaa
    18241 tagaggatat tgcacaagat ttttctaagg aaaagtatgc tctattgaga tacgcattag
    18301 aaaatgttaa gttaaaatca agacatgtag ttttttgttt aaatacaaga gatgtaattt
    18361 taaaacccca aaaacttcct aatatggaca aaaaggattt agatggattt atgaagctag
    18421 agatggacga aatgatggct ttggagtcag atgaatatgt attttcgtat gaagtatcta
    18481 gtgaaacacc tgataattca gatggcgatg aatcaagctt aaatcttatc attggagcta
    18541 tacaaaagga agaggtaaat gcaattgtag agataatgca tgaatttgat ttagtattag
    18601 atagaataga tacattatca acagcttact taagaatgct taaaaattta gactacaatg
    18661 atataatggt aaccaatata agtgacaata gcacaattat aaatatatat aagaaagata
    18721 ctttatttat tagtgataat atacctataa agataactgc taaagatatg gacatgcaat
    18781 tattaaatgt agtagaagaa tcaaaaggtc ttatgaatta ctattcgtca agaaactttg
    18841 gtaaggttac ggatactata ttaatattag gaaaaagata ttccaataaa ttaatttatg
    18901 aaacttttag ctctgcattt tcaagctatg tttctcaagg ccttactgag ataatggata
    18961 tatctgatat gataactgga gatatagatg aagatgatat aaattcaatt gtagagcttt
    19021 taggaagtat gatagaacct aaagggaaaa gagattttga aaatataaac tttttaccaa
    19081 tggatatact tagaaaacag ataaaagcta aacaaatgaa aaacatactg aaaattgctc
    19141 caatagttat tttgattctt gctatacctt atttatgctt aattttttca aataatttag
    19201 caaataagga acttgaaaat gttaatggtg aaataagaaa tattgaatct gcatattatc
    19261 agattggaaa tatagaaaaa atgatacaga gtaaaagcga agaaataaag gtatatgata
    19321 tgttaattgg gaaaaaggta aagtgggaaa atttattgga tgatatagat aaaaatactc
    19381 caagtaaagt tgaactaaca agtttatcaa caacttatca aaatcttgga actaataata
    19441 aagctgataa ttcaaaaagt aataagacta caacagataa atcaagtaac aatgaaacaa
    19501 aatcaagtac gaaaagtgat agtaatggag ataaaacaga taatgattta aatgaagata
    19561 agactacaaa tgtgtcagaa gcattaactg gaaatagtga gactaatagt aataacacgg
    19621 ataaagatga taaaactcca ttgtatgata agataccaaa tgttataaat atagaaggaa
    19681 atgctaaaga ttcctcttat gtaggtcaat ttttatataa attaaaggga ttatactatt
    19741 tcaaggatgt taaattacat agcataaaaa gtaatgcaga agattctaac tatacattta
    19801 gtatgacatt atacctgaaa gaaggtgtat taactaatga ataaagatat atttaaaaaa
    19861 gatataaaag atatatttaa aatggaagtt agtctaaaag ggattcttat aaaggttata
    19921 attttattag ctattttatc atatggttat tttggacttt accctcaata tcagaaatat
    19981 attgctacaa aaaattctgt aaaacaggct actgaaaaat taaatatata taaagagaaa
    20041 actgcactta tgccaaaata tgaaaaacaa cttgaagaag ttgaaagtga attaaaagaa
    20101 aaaagcaaaa atcttttata tgatatgcaa gatggtcttt ttttaatagg tttagataaa
    20161 aaaatgaagg cttataatat tgacttaacc agttatgacg ttggaaatgt taaagaatac
    20221 gaacattttt atggtatatc aacaacaata agtgttagag gtgattatag aaatgtcaaa
    20281 aaattaatgg cttatttaga agaacaaaaa aatgttactc aaatactaga ttatacaatg
    20341 acttcatata tagaaccaga aaaagaagag gaagaaacaa caaacaataa ggatttaatt
    20401 aaaaatgtga acataaataa agatacaaaa atatactggg tggaaggcga aaataagtgg
    20461 catttatata aggattgtcc attgataaaa gagggagaga ttataaaaaa tgacaaactt
    20521 ttagataggt ctactaatta tctatgtaat acttgtgtac ttagagctgc aacagaaaaa
    20581 gaagcaaaaa aagaagtcgt agctccaaag gcagaaggtg tagtagatgc tacatttaaa
    20641 tttataatgt acactaaaga gaatactaca atgatacttg atacagataa tccatcttca
    20701 tggaggccag gaaaatataa tccatttaag tctagtgcga aataaagcta agtaagcttt
    20761 aaagataggt gatgagtttg tacaaaaata atgaaaaagg tcttacttta ttagaagtaa
    20821 taatagctgt ttttatactg acaatagttt taagtatttc ttataaagtg ttcaatggta
    20881 taacatcagc agtaaaaaag caacagatta ttacagatgc tcaagtaaat attaatctaa
    20941 ttaataagta tctaaataga gatttggaaa actgtaaaga actaactaaa actggttcag
    21001 gtaataatta tgaatacaat atagagatgc cagataatgt tgtaaaatat gaagtttcta
    21061 tagaaactaa aaaaaataca gaggtatatt ctgtaacgag aatccaaaac aatacaattg
    21121 atacagaaaa tgaagttaga gaagaaataa tctataatca accactagtt caaaacaata
    21181 aagaaatgaa ggaaacacca tttaaaatag aaaagcaaac tggtaaatct atttatactg
    21241 taagtatata ctataatgaa tcagtgcaag aaagtcataa aaatagcaat ttaaataaca
    21301 aaacttatac atttgatgtt atgtcaagaa tagggtaata agaatttatt aaaaaataag
    21361 taggttgata aaatatgagt aaaaagaata gtgaaagagg attttcctta attgaagtgt
    21421 tagtagctat ggctattatg ggaattgtac tatttgcatt ttttaatatt atcaatacta
    21481 ataacaaagc aaacactaaa aatgacacag atataacttc cttaaactat gttcaaagtg
    21541 aaatagaaaa tctaagagaa aagataaaaa gtggagaatt tgattttgat agtttagata
    21601 aactagaaga tggaactgtc gtatatgaaa aattaataga taaatcaaaa aaagttgtat
    21661 atgataaagt tcttagtgaa ggtgatgtga gcttatatga tactccatat gaaaagatta
    21721 caacaataaa agatgaagat ggtaatttaa tcgacaaagg aaatattaca aataagataa
    21781 agactattgt agaagataaa agtggtcaga tatataaaat agctgtaact gggaaaagca
    21841 tgaatgacta ttcaagtaaa aaagaggtta aaattgtaac tgaaatattt aaagataaat
    21901 aattttactt gggggtgaga ttttgaggaa gtggaataaa tttaaaagtg aaagaggggc
    21961 agctttagtt ttagttttgg ttgtagtagc attgctttct attgttggat tgatattttc
    22021 aaatcagata gctaatagaa taaaatctac aaagactact aatgaaggta tacaagcaaa
    22081 gtatttagca gagacatgtg tggaaaatag tattgataaa gcatatgaaa aattgtatga
    22141 tgagcttgag aaaatggata atgagtttaa aagtgaaaat caagaaaaat ccatatctag
    22201 gtcaaaatta agaaatatat cagatgaaga ttttaataat caagatgagg aaaatattga
    22261 agcagaaaga cttggatata tgaataatat aaatttctat ttaaataaag cttcttcaga
    22321 cttggaaaaa gctagtatgg aattgaaaaa gctatatgat ttagatatgt tggactatag
    22381 agacattgaa tatgtagatg caaatattat ttcacataga gattctatct tagaaatttg
    22441 caagaattat acttctggag atattagtaa aataaatgaa tatattctga aagaagatat
    22501 agactcaact acattgattg aggcaaagtt agtgaataat gatattcttt taaaaatgtt
    22561 tttagaagaa aataaaattg aaaatgaaca tttaaattca gccttttcac atacttataa
    22621 ggcattagat aacatttctt tggcgatgca aaatatgatt gagtatagac atacgtttca
    22681 tatagatgag ccaaaagtag aagtatctaa tgggattcca gactctcaac aatattatga
    22741 actaatacaa aatcctataa taaatagtat ggagtatagc tggaatagta aatgggatac
    22801 attagaaaac ctattggaaa ttttaccaaa tcaaactcaa ggatttaatt ctttaagagt
    22861 tcatttgaga aataatgtaa gaaaatttga gaagttaagc gataatatat ctagtggtaa
    22921 aaaaaataca gcaaaaaatt tcttaaagta taaagagtta ttgtatgaaa tatcagacca
    22981 atgtaatcaa ctaaaaagta tgtcttatga gaaaatacct gttaaatatg ataacatggc
    23041 tttgataaca acttttgact atatacaaaa tgagctttta gcagagataa aatgcagatt
    23101 aaaagaatta aaaccacaag aaattgataa aacagaaggt attactataa aaataccttt
    23161 ttataaggct gactacgaca tgacaaaaga aggatggcct aaattaaaag aaaatggttc
    23221 aggggcggag ttaagcttaa tggtaactgg tgataaaaat ggaataaaag aagtagaagt
    23281 tacagatggt aaaaaaaata ttattggttt aggtgttgaa gaaaattcaa attcaaagta
    23341 taaggttgac gccattgtaa actttaatct aaatatagat acaaatgtag ttggaaatta
    23401 tgatataaaa gataaaattt tgattaatca tgatataagt tcatacaaaa aagtaaacta
    23461 aatataagaa gcaacttagt ataaataaat aataaaataa ttttattatt tactactaaa
    23521 aaaataagta aactaataga agatatggag agtaaatatt atgtatattt atgatttatt
    23581 agagcaaggt ataagactaa acgcatcaga tatacacata acagttggaa caaatcctgt
    23641 agctagagta aaaggtggat ttgtaaaact aagtgaacaa atattgacat cagaagtaac
    23701 aatgcaaatg gcaaaagaca tagcagggga atctatgttt aaagtaatag aagaacatgg
    23761 agaagctgat ttttcagctt ctctaaaaac aggagaaaga tttagggtaa atgcatatag
    23821 gcaaaaagga aattacgcaa tagcaataag gactataaca gcagaaattc caacatttga
    23881 aaaattaggc cttccagaga gtataaaatc ttttactgaa aagcataaag gattagtttt
    23941 agttacaggt ccaacaggaa gtggtaaaag tacaacactt gcaagtatga taaatataat
    24001 aaatgaaaaa caacaaaaac atataattac acttgaagac ccgatagaat atgtacatca
    24061 ccataaacaa agtttagtaa atcaaagaga agtaggaact gatacagaaa gttttcattc
    24121 agcccttaga gctattttgc gtcaggaccc agatgtaata ctaataggag agatgagaga
    24181 ccctgaaact gtttcaatag cattaacagc agcagaaaca ggtcatttag tattttcaac
    24241 actacataca gtgggggcag ctaagacaat tgatagaata gtagacatgt ttcaaccatc
    24301 ccaacagcaa caaataaaaa cacagttatc tacagtttgt gaaggtgtgg tatcacagca
    24361 gttacttcct acagcagatg ggaaaggcag aatagcagca attgaattga tgtttgctac
    24421 tccagcaata aaaaacctga taagggaagg caaaacatat caaattccaa acatgataca
    24481 aactggagtt aaagtaggta tgaaaactat ggaccaagat ttaatggaac tttataaaaa
    24541 tggaaagatt acaaaagata tggcacttag taggtgtact gaccaagagt ttatgacaag
    24601 aatgataggt ggagtaaact acaatggata ttataatcgt taatctatat gtatttatag
    24661 tgggtatagt gtttggaagt ttctttaacg tctgtatttt taggattcca gaaggtatat
    24721 ctatttctag accaccttct cattgtatga gttgtaatac tagactaaag ccaatagact
    24781 taataccagt gctaagctat ttaatgagtg gcagaaagtg tagatattgt tatgagaaaa
    24841 tttctagtag atatgctata gtggaattaa ttacaggact tttattttta acagtattta
    24901 tgttatatgg aatatctaca tcaactattt attatttggt tcttgtgtct ttactgatta
    24961 taattacatt tatagatatt gaccacttta taataccaga taaaatattg gtttttggag
    25021 ctatattttc tatgattttt aaccttttat ttaaagaaat tccagttaag gattcaattc
    25081 taggtgtttt tatttgtgga ggaagtgtct ggattatagt tcttttgatt gaatttgtaa
    25141 taaaaaaaga gtgtatgggt ggaggagata tcaaactatt tgcaatgcta ggactttaca
    25201 tgggtgttaa aaatggtctt ttgacagcct tactaagcgt ttatgtagga gcagtctatg
    25261 gaatctgtgt aataattata agtagaataa aaggaaagga atataattct gttataccat
    25321 atggaccttt tatatctatt ggagcattaa taacaatctt atctggaaag caattacttg
    25381 aactttattt ttctatggtt atatagatga tagtcaagtt aaataaagtt ttaatttaaa
    25441 aaaatgaatt aaagtattta ataaggacaa tattattatt aaccctatta ctaaagtgct
    25501 acaaactatg gagtatataa taataatttc cttatttttt gcacatttga taaataaatc
    25561 tctatgtgtt ttttgtgata gagaaaagaa aaatttatat aatacgtttt ttatttacat
    25621 aataactgta gttttatctg tattaatata tctaaaattt gggattagtg tagaatgtgt
    25681 acaatatatg gttttaattc catttttaat aattatatcc attgtagatt actacacaat
    25741 atatgtatat gatataacaa tagttagtgg tataatagtt caaggtataa tttttttggc
    25801 ctttttcaat atagctcaat ttgattatat aaatcatata tttggactat tgataggctt
    25861 tattatacct tatattattg ctaaactaac aaaaggatta ggctatggag atgtaggtct
    25921 ctatagctta tgttgctttg cattgggaaa taattatggg ttttatgtga tagttttatc
    25981 atttatttta gcgtttttgt tttttattat tttatacata actaaagtat ttaaagatgt
    26041 agatataaga aagcaaattc cttttacacc ttttatttcc ttgtcaacaa ttttaataat
    26101 atttacagag tataatatat ttaattttta ttataatttg ttatacaaat ttatataaca
    26161 ggaggtaaga aatgtacgta gtagtagggt taggtaatcc aggaaagaag tatgagaaga
    26221 ctagacataa cgttggattt gatgttatag atattcttgc taaagaatac aatattagtg
    26281 ttactaaaat aaagcataaa gctttgattg gtgaagggag agtaggtact gaaaaagtat
    26341 tattagttaa accacaaact tatatgaatt taagtggaga aacattaata gatatatata
    26401 aatattataa ggttgattta agtaatatag tagtagtata tgatgatata gatttagaag
    26461 ttggaaagat acgtataaga aaaaaaggaa gtggaggaac tcataatggc atgaaatcta
    26521 taactaagtg cctaggttca aatgactttc caagagtgag agtaggtgta tctaaaccag
    26581 aagctggtca agacttagca gactttgtac tttctagatt tagaaaagaa gaatctgata
    26641 acataaatga agcattagaa aaagcggcag atgctattga ctcaataata agagaaaata
    26701 tagatatgtc tatgaacaaa tataatggat agtgagaggg agatttaaat gaatgatgtt
    26761 tttttatatc ctttgcaaaa ttcaaaagag tataaagata taataaactg tataaaaaat
    26821 acaaaaggct cattattggt taatggactt ttaccagtac aaaaacctca tatatcgtat
    26881 tctatattta atgatttatc acgacaaatg atttttataa cgagtagtga tttagaagca
    26941 aagaaagtat acgaagattt aagtttttat atggaagata aagttgaata tttaggtttt
    27001 caagatattt atttttatca cttggatgca aaggacagaa acgaagaagc aaagaaatta
    27061 aaagtattgt taaaactagc aaataaagaa aaaatcattt tagtaacttc tattgaagca
    27121 gttttaagaa aatacatacc aaaacaagtt ttgctcgata gtgtatctca ttataaggtg
    27181 ggagatagct tagacttaga aaagttaact gagaaattag tttcattggg atatgaaaga
    27241 gtttcaaaaa tagaggggtt tggtcaattt agtataagag gtggaattat tgatgtattt
    27301 tcacttgaat atacaaatcc aattcgtatg gaattatttg atgatgagat agattccata
    27361 cgaacttttg atgtatattc acaaaaatca attgataaat tgcaacaatt ctctataact
    27421 ccatctaggg aatttattta tcccgaaaaa actacagatg cattagttaa attaaaaaaa
    27481 gaaacaacta aaaatactga tgaaggtaca tttcaaaata tagattatat atcaagtaag
    27541 acttattttg aaggtgtaga gaactatata gattatattt atccagaaga aaacaaaagt
    27601 atatttactt atttagcaga tgatgcaata gtatttatca atgatataac gagacttaaa
    27661 gaaagatgcg aaaattatat aaatgagttt agagaaaatt ataaattaaa tcttgaaaga
    27721 ggtctggcta ttaaaaacca aggtaaattg ttatatcact atacagattt agagtactta
    27781 gtaaaagaca agtctgtggt attaaactca ttattgccaa agtctataaa taacttttct
    27841 ataaaatcta taataaattt tgaaagtaga gaagttccta catttaatgg aaaggtagat
    27901 ttgcttgtag aagaattaaa tcggctgaaa tataatggat acaaaataat tttagcaaca
    27961 aatacattag aaagagcaaa taaattagga aaagatttat tggacaaagg cttagaaacg
    28021 accatttcta aagatagaga tattgaaatt aaatcttcac aagttataat tgtacctgca
    28081 cacataaaca gtggttttca gtataaatct ataaagtttg ttgttataac agataacgaa
    28141 atgataggtg tttataaaag agcgtctaag actagcaata aaaaagttaa aaagggaaag
    28201 aaaatagaat cctttttaga tttaagtgtt ggagactatg tagtccatga aaatagtggt
    28261 gtaggtagat acacagggat tgaacaaatt acagtcaatg ctattaaaaa agattatatg
    28321 aaaatagtat atcaaggtgg agataatcta tatgtgccaa tagaccagat ggacaaagta
    28381 caaaaatata ttggtgcaga agtagaaaaa gttaagttaa ataagcttgg aacaaatgag
    28441 tggacgaaag caaaagcaaa agttaaaaaa gaaattgaag atatgacaaa agacttgatt
    28501 gagctttatg caaaacgtga aaaaatacaa gggtataagt tttcaaaaga tacaccttgg
    28561 caggcagagt ttgaatcgtt gtttccatat caagaaacag aagaccaatt aaaggctata
    28621 gaagaaacta aaaaagatat ggaatcaagt aaggtaatgg ataggctagt atgtggagat
    28681 gttggttatg gaaaaacaga ggtagctatt agaagtattt ttaaagcttg tatggaccaa
    28741 aaacaagttg ctgtattggt tccaacaaca atattggccc aacaacatta taatacattc
    28801 aaagagaggt ttgaaaatta tcctttgaga gtagaagttt taagtaggtt taaaacacca
    28861 aaacaacaaa aacagattat tgaagatgct aaaaaaggat tagtagatat tttaattgga
    28921 acacatagaa taatatccaa agatataaat ttaccaaact taggtcttgt agtcattgat
    28981 gaagaacaaa gatttggagt aaaacataaa gaagctttaa agaaaattaa atctacagta
    29041 gatgtactta cactttcagc aactcctata ccaagaacat tacatatgtc tttaagtgga
    29101 ataagagaca tgagtgttat agaagaacct ccacaagaaa gacatcctgt aataacatat
    29161 gttacagaga gtaaggaaag cgtaattcaa gatgagatag agagagaatt aagtagaggt
    29221 ggacaggtat tttttgtata caatagagtt gaacacattg aagaaatggc aagtatgatt
    29281 caaaaactcg taccagatgc tagagtggct gttgcacatg gaagaatgac ttctaaatca
    29341 ttagaaaata taatcctagg atttttaaat aaagattatg atgtactagt ctgtactaca
    29401 ataattgaaa ctggtatgga catatcaaat gcaaatacaa tgattatata tgatgcagat
    29461 aaaatgggat tggctcaact ttaccaattg agaggtaggg ttggaagaag ttcaagacaa
    29521 ggatatgcat atcttttgta tgaaaaagat aaaactttaa gtgagatagc ggagaagaga
    29581 ttaaaagcta tcagagaatt tacagagttt ggttctggtt ttaaaatagc aatgagagac
    29641 cttgaaataa gaggagcagg aaatatttta ggttctcaac aacatggtca tatggcagtt
    29701 ataggttatg atttgtacgt taaaatgctt aatgatgcaa ttaaaaaagt caaaggtgaa
    29761 cctattgtgg aggaaattga tgttgaaata gatttaagtg taaatgcata cattccagat
    29821 aattatatca aagatgagtt gattaaaatt gaaatgtata agaaaatagc ttctattgaa
    29881 aacaaagaag acatgttgga tatacaagaa gaacttgagg atagattctc tgatatacct
    29941 aaaccagtcc agacacttct tacaatagct tatataaaat cgttatgtaa gatattaaaa
    30001 atagaaaaga taagacaatt aaaagatgaa atattattag ttccaataac aaagtataga
    30061 actaaacaga aaataggtta taatatagtt acagaattag aagaattatt agaaaagatg
    30121 tgcaaggtaa aataacttgt aaatttaaga agggatgttt taaatttgaa aaaagtaata
    30181 acattggtaa tagctatgat tttggtagta tctgtgactg cttgcagttc tagtaaagga
    30241 gaaactgtgg caacagttga aggtacaaag atatcatcag atgaatttaa aaaaactata
    30301 gctctttata aagattctat ggaacaaact tatggaaaag atatatggga taaggaagtt
    30361 gaaaagggtg taaaatacaa ggacaagttt aaagatttaa ttcttgacca acttataact
    30421 acagaagtaa tatattctca agctaaaaaa gataacttac ttcctaaaaa agaggatgtt
    30481 gaaaaaagtt ttaaagaatt aaaagacgct atgggtaaag atgaaaaata taaagaacaa
    30541 cttaagaaat taggtataga tgatgagttt ttaagagacc aacaagagaa agatttagca
    30601 atgcaaaact atcaaagtaa ctttgctaaa aagactaaaa tatcagatga agaaatgaaa
    30661 aaatactatg atacacataa agatgaattt aaaaaagatg aggtagaagc ttctcatatt
    30721 ttattgaaaa cagtagatga taataacaag ccactttcag ataaggaaaa agctgaagct
    30781 aagaaaaagg ctgaagaggc tcttaaagaa gttaaatcag gtgaggattt tgcaaaagta
    30841 gcaaaaaaat attcacaaga tacttcagca agtgatggtg gtaaattagg attcttttca
    30901 aggggtcaaa tggttgctga atttgaagat gctgcttttt ctatgaaaaa gggtgaagta
    30961 tctgatttag ttgaaactca atatggatac cacataatta aagtgacaga cagaataaat
    31021 gagcaaacat catttgaaga tgctaaagaa actataaaag accaactgtt gaaaaataaa
    31081 tatcaagaac aaatagaaaa attaactaaa gaagctaagg tagaaaaaga tgaaaaagta
    31141 ataaataaaa tcacaatcta agacttaaaa aaagagtaat atgcttaaaa aagctatatc
    31201 tggataagat atagcttttt ttgtatattt ttcttaacaa tggtaaaaat aaacttaaag
    31261 taatttatgg agggattaaa aaatatgaaa gcaacaggta tagttagaag aatagatgat
    31321 cttggaaggg tagttatccc aaaagagata agaaagacat taagaattag agaaggagac
    31381 cctttagaga tattcacagc aaaagatgga gaagtcatac ttaaaaaata ctctccaata
    31441 ggtgaattaa atgaattttc gcaagaatat gctgaaacat tagctgaagt actaggatat
    31501 ggagtgttga taacagactt agattctata atagctgtgt ctaagcttcc taaaaaagat
    31561 tataaagaaa aaagtataag tgatgagtta gaagcaataa taagtgaaag aacagtaaaa
    31621 tattcaaaag ataaaaacat tttaccatta ttcaaggaag accaaaaaga atatactagt
    31681 caagttatca tgcctataat tagtgcatct ggtgactgta taggttcaat tgtagttgtt
    31741 tcaaaggata aagatacaat tgcagaaagt gaagaaaaat ctttaaaaat tgctgctaac
    31801 tttttaggaa aacaagttca ataattttat cataaagacc ttttacatga aggtcttttg
    31861 aagtttttgt gtaaaatatt gaatatagca ttaaaaatat ggtatattaa agtttgttat
    31921 ataaacagta tggaggttat gttatgaaca ataacaaaaa taaagactcc tttctaaaag
    31981 gagcccttat acttgggcta gcaggtgtaa tcgtaaaaat aatgggtgga ttttttagga
    32041 ttccccttgg aaacatgatt ggttcagaag gaatgggata ctatcaggct gcttatcctg
    32101 tgtatactct atttttaaca ttagctactg ctggatttcc aacagctgtg gcaaaattgg
    32161 tttcggaaaa agttgcaatt ggaaatttta aaggtgcaaa taagatattt aaggtatcac
    32221 atacagtttt attcattacc ggtataatat cattttgtat attatttttt ggagccgact
    32281 acatagtaac aaacgtaatg aaaaatccag gtgcgttata ctctatgaaa gccattgctc
    32341 cagcattact atttgttcct gcaatgtctg catatagagg atattttcaa gggagacaag
    32401 atatgactaa gattgctgtg tctcaagttg cagagcaatt ttttagagtt gtattaggtc
    32461 ttactttggc ctacttgctt atgaagagtc taggtcagac ttttggtgca gcaggtgcta
    32521 tatcaggggc tacaataggt tcaatagcat ctatgcttta tttggtgttt gcttatatgc
    32581 tagggagaaa agagcgtaga gcagagatag atgcaagcca aagatttaaa gatgaaagag
    32641 tgtcttatat ttttaaaaag cttttaactg ttgccattcc tataacaata ggagcatcag
    32701 taatgccact tgtaaatatg atagataatg ttattgtaat aagaagactt atggaagcag
    32761 gctttacata caaagtggca aattcaatgt ttggtcaatt aacaggtatg gcaatggcca
    32821 caataaattt accagcagtc ataacaactg caatgagtat gagcttagtt ccagcaattt
    32881 ctaaagcata tgcattagga aataagtcaa aagctagaaa agatactaag agtgcagtta
    32941 aggtaacttt acttatagta ttgccttgtg cgtttggtat ggcttcttta gcaataccaa
    33001 taatgggtct tttatttcca catgaaccat cttctgtagg aactattttg tttactctta
    33061 ctccatgtgt attattttta ggattaatac agacattgac tggtataatt cagggaatgg
    33121 gaaaaccaat agtacctgta attgctttat gtgttggtat gctatgtaaa attgtaataa
    33181 gttatacttt aacaggtata ccagatatta atgtactggg ttcagcattt ggtacagtta
    33241 cagcatactt tgtggcagcc atgattaact tactatatgt taaaaaacac atgaatgtaa
    33301 acttttctaa aaaagaattt ataataaagc catttataac agttatgaca atgtttataa
    33361 tggttaagtt gagttatggt gctttagttg gattcttagg aaacagcata tctacaataa
    33421 ttgcaatatg tgttggtgga attgtatatg tagtagtaat acttggaata ggtggaatta
    33481 aaaaagaaga gattctaact atgccaaaag gtgataaatt atataaattg ttaaaaaaag
    33541 ttaaacttat aagataatat aggaatatat atgcaaataa aggaaatact atacaaatag
    33601 taaatctcct tatttgtata ataatataaa aataaagtga aatttgctaa taggagtgat
    33661 gttatgggta agataagtat tgtaggtctt ggaccaggag actattctct tataagccaa
    33721 ggtgtgttag aatctttaat aaatagccct aaagtttttt ttagaacaga gaaacatcca
    33781 ataattgata aacttaaaga aactatacaa tacacttctt tagactactt ttatgaaaaa
    33841 gaggaaaatt ttgataatgt ttatttgaaa atatctgaat ttatcataga agaatcagca
    33901 aagggtgacc tagtgtatgc tgttcctggt catccaagag ttgcagagaa gactgtaagt
    33961 ataatagaga gattagccaa tgaaaagcac atagcagtag aaatattgcc ttctatgagc
    34021 tttgtagacg ctatgtttaa ctatttggct atagacccat ctgagggatt taaactggta
    34081 gatgcttttg aaatagaaaa ttcatatata gacttggata caagcatgat aataactcag
    34141 gtatatgata aatttatagc atctaatgta aagttaaaac ttatggaaca ttatgactat
    34201 aatcaagaag tatgtatagt aaatggagca ggtgtcaaag atttagaaag taaaaagttt
    34261 ataaagcttt gtgaattaga cagaaatgaa aacttttttg actatttaac tagcttatat
    34321 atacccaaaa gttctaaaaa gatgtataat acagtacatg acttagaaat aatagttaat
    34381 acacttagaa gtccatctgg atgtgaatgg gataaaaaac aaacacatca atcattaaaa
    34441 aattctgtta tagaggaagc atatgaactt tgtaatgcaa ttgataataa tgatatagat
    34501 gaaatggtag aagagttagg tgatgttctt ttacaggtta tattccattg tcagatagga
    34561 aatgaggagg gctactttga tttaaaagaa gtagtaaatg gcatatgtaa aaaattaatt
    34621 catagacatc ctcatatatt tagtggtgca aaattagata tgtctaagtt tgagaaaact
    34681 tgggaagaat taaagaaaga agagaaaggc gaaagtacta taacagatgg ccttagaagg
    34741 ataccaaaac atttaccagc tcttatgaag gctagtaaag ttcaacataa agcagcactt
    34801 gtcggctttg attgggacaa tgtagaagat gtatttaaaa aaattgaaga agaatatcag
    34861 gaattacttg acgaacacag ggtcgggaat ataaaataca taaaggaaga gctaggcgat
    34921 ttattatttt cgatagtaaa tctagctaga tttttagaca tagactcagg agaagccttg
    34981 aactgtacta cagaaaaatt cataaataga tttgacttta tagaaaaaag tgcaatgaac
    35041 ttgaataaaa aattagaaga catgacactt gaagagatgg atgagttttg gaatcaggct
    35101 aaaaaacaat aaaattgtgg aaggaaattt gtcaaaaatc tagaactttt atcacaaaga
    35161 tagaagtcta tattataaac atttttagga ggtaaatatc gtgaataaag ctgaattagt
    35221 atcaaagatg gcagaaaaaa gtggattaac aaagaaggaa gcagaagctg cgttaaacgc
    35281 atttatgagt tctgttcaag atgcactagt aaataatgaa aaagttcaat tagttggatt
    35341 tggaacattt gagacaagag aaagagctgc tagacaagga agaaatccaa gagatccaga
    35401 gcaagttata gatataccag cttctaaagc accagttttc aaagctggaa aaggattaaa
    35461 ggatataata aatggataat tagaaatctt gtgaaaaagt gtggataaat ttgccacact
    35521 ttttctatag aaccaaggaa ggtgataatt atgagattag ataaattttt aaaagtatct
    35581 aggattataa aaagaagaac tgttgctaaa gatgcatgtg ataaagggat tgtcactata
    35641 aatggaaaag ttgctaaatc ttcttcagag gtgaatattg gagatacatt agaaatacaa
    35701 tttggagaga agaagatgaa gtttaagata aatgaagtaa aagagcatgt attaaaaaat
    35761 gatgcaaaag aaatgtacga aattttagaa taaaattaat agtcctctag tatattaata
    35821 gtagtattat tgaaatattt actgggggga tattatggaa cagaacataa ctttgaaaga
    35881 tagatcaaag ctggttattt cgggagtcga gcacatatac tcttttaatg acaaaagagt
    35941 agagctcaaa acttctgtag gcgagatggt aattgaaggc gaaaacctag atatgagcaa
    36001 gctaagtata gacgaaaata taattagcat agatggaact ataaattcta tggtatatgc
    36061 aaagcctaaa aagccacaag agagtttctt gaaaaaggta tttagataga tgataccttt
    36121 tactcaagat gtaagcattt tttatgctac tatttatggt ggaatactca taggggtgtt
    36181 gtttgatttt tatagagggc taagagggaa ttttaaattc atcaattatt ttgctataat
    36241 ctttgatgta ttgttttggt ttctagctac ggttataata ttcgtaacaa taaaccttac
    36301 agaatttttt gatttaagat attatcattt tgtggctttg tttattggtt ttattttata
    36361 ctataacacc ataagtaaaa ttgttttatc aattatcaat aaaataattc gttttgtgag
    36421 aaattctttt aaaaaagtta cacattacat agtcagtttt ttaaataatt tgtattacgt
    36481 tataatctat tcactacacc tactatttga tattatattt tatataccaa acatatttat
    36541 agcaacaaga aaaagtataa aaagaaaatc aaataaaaaa ttaaagaaca agaaaaaatc
    36601 aaaacctaag aagaaaaaaa ataaaactaa aaaaagggtg taggaaaaat gaacttaaga
    36661 aaaaaatttt caggacagat tattgttatc agtttatttt taggaatatc aatttttagt
    36721 atgatgacag gatttgtatt tgagtataca aaagctaagg aatataaaaa agaaatagca
    36781 agtctaaata aacaattaaa aaagactgaa atacaaataa acagcttaaa gaaagatgaa
    36841 aaatcatatg aaggagactt agaggatata gcaagaaaaa gattgaatat ggtaaaacca
    36901 aatgaaacag tttatgtaga cataaatcgt tgattaaaac ttgctaataa gtttacagat
    36961 gtattgattt taaaatttaa gatttaataa aaaagtcatt cagttaagga tggctttttg
    37021 tttttattga ggagggaatt tcataatgaa agtaggagct atagatattg ggacaaactc
    37081 aatgagatta ttgatagcag attatagaga tgggaatttt ataaacagaa aaaaatttgt
    37141 taatacaact agaattggtt atggtgtgga tagtaaagga tatataaaag aagatgctat
    37201 caaaagaaat ataaaagcat taaaagaatt ttctgataag tgtaatgatg aaaagtgtga
    37261 aaaaatttat tgtatgggga catctgctct tagagacagt aaaaatggag attactttgt
    37321 taaattagct aaagaagaaa ctggaataga tgttgatata gttacgggaa tagaagaatc
    37381 aaatttgggc tttaagggtg ttttagaagg aatacaaggt gatgatacta ttattgttat
    37441 agatattgga ggaggttcaa cagaatttat tgtaggtgat tcagaaggaa ttatatttaa
    37501 taaaagtgag aatgtaggtg ctcttaggat gacagagagg tttttgaagc acgatattat
    37561 tgaagatgaa gagtttgaag ctatgagttc atttatatat ggtgagattg ataaaacgtt
    37621 aaatcatata aaagacagag gattaaaaac tcttataggt ataggaggta cgattacatc
    37681 attatcagca atgaatcaag aattagagat ttactctatg gaaaaaatac ataatagtaa
    37741 aatacacaaa aaagaaatag aagaaattct acaaaaatta ataaagatga cattaagtga
    37801 taaaaagaac ttaaaagggc tacaagaaaa acgtgcggat attataactg ctggtgtaaa
    37861 aatattgaat ataataatgg aaaaattaga aatagaagaa ataaccgtaa gtgaatacga
    37921 taatttggaa ggcttaatat gtcaaaagtc aaaaaaaatg tattaatatt ttttgatttt
    37981 taaaaccgat tatgacaaaa aaaattactc ctctttgtta caattcttaa tataaacaaa
    38041 agaggaggtt tttttatgca aagaagtgta gcagttgtga agaataagac tgtaaaacca
    38101 aatgtcataa aattaataag cggatatatt gattttacta cggcggcatt cgctgtgatg
    38161 ggatttttac ttagtaggtc tattatagta gactctgtag ctcctttagg aattgccttt
    38221 tttatatgtg catcaaagat tgataagtac aagatacctg tattcatatc aacattatta
    38281 ggaatattgt tatcatctaa tgattttgga aatgtagcaa aatatgctgt gtgccttaca
    38341 atatttatgg ctatgaataa aaaattaaaa ttatcagatt caattgctaa ggttgcattt
    38401 ataggtgcaa taatactact tccaatctcc atgggacaag ctttcttttc aaataggtat
    38461 gtatatggtc ttcttatagc aggagtagaa gcaataataa tgtttatatc tatatatgtg
    38521 ttttcttatg ggataggttt aatggccaat gtcaacaata ggatgtctgt acggacagaa
    38581 gaagccatat caataagttt actgctaacc tttagcataa tgggaatagg agatatatct
    38641 ctgtttggaa tatcaataag gaccatttta tcgaccgtat tgatattgct gacctcaata
    38701 gtaggtggag ctaccatggg agcatctagt ggtgtaatta ttggtgtagc atccatttta
    38761 aataatatga catcagctgt ttatatgggg atatattctt tttcaggatt aataagtgga
    38821 gcatttaata aaataaataa atatttctgt atactaggat atattctaag ttggacaata
    38881 atatacctat atacttctgg tattacttct aatatgatgc aattaagaga tatactatta
    38941 ggttgtttga tagtactagt attgccagag cgattattta ataagattga aaaattaata
    39001 aaaagtaatg tggcttcaaa tgaaatcgta tacgattata ttatgagaag caaaaaccta
    39061 actaatagca gattgaacag tatttataaa acttatgatg accttgcaga tacatttgat
    39121 aagataagag aaaaagacaa agtgctagac caaagagaca tagctaatgt aatagatatg
    39181 attcataatg atgaatgtaa aagttgtagt atgagaagaa tgtgctggga gtcaagattt
    39241 aatcatacat acactatgat atataacatt cttgaaaaaa ttgaggagaa aggggagtta
    39301 agtctaaacg atatacctaa aaattttaga aaggaatgta tgaaaccaga gtcaatagtg
    39361 aaaatatcaa atcactatta caaaatgttt gtattagatt atgattggag tgtgaagttt
    39421 tcagaaagta gaaagttaat agcaaatcag ataagaagta tttcaaaatc cattaaatcc
    39481 ttatctcaag atttagaagg cgacataatg ctagatatag aaaaggaaaa aaatatatat
    39541 gaacagttag aaagatatga tataacagtt gataaagtaa gttacttaac aaaaagtaac
    39601 agtgagtttg aaatatcaat agaaaagaaa acttgccatg atggatgtat gtgtgaagat
    39661 aaaattgtaa atataatttc agacctagta ggtgaaaata tgtctgttag aaaaatagga
    39721 tgtcactgtt taggaggtaa atgcaaggca acattcgtta aatctcaaaa atataaggct
    39781 gttacagaag tttcagcaat gtcaagggat gggcatatcc tttgtggaga caattacaca
    39841 tacatggaga taaatgatgg taaatacatg atggctataa gtgatggtat gggaaagggt
    39901 aaaaaagcat atgaagaatc ttctgccact attgatatac tggaaaaaat gatagatgca
    39961 aaaattaaag atgagatagt cattgatact ataaacaata tgttgttgtt aaaatcttca
    40021 gaagagatgt tttcaactct ggatttggga attttagatt taaaacgagg ttgtttagaa
    40081 actataaaga tgggagcgtg ttctacttat ataaaaagag aggatggaga agttgactta
    40141 atatcatcat catcattacc tgtgggtata ttatcagatg taaaaattga tagaaaaaat
    40201 gtaaaagtta aggaaggaga ctatgttata atggtttctg atggaattgt agatgcagga
    40261 agaaataaca atttaggaga caattggtta atatacttct taaagaatat agagactaca
    40321 aatccaaagg aaatttcaaa tttaatattg gatagagcat tagagttaca agcgttacaa
    40381 atcgaagatg acatgactgt tttagttacg aaaatatgta caaaataagg tttacaagat
    40441 tatgtgtata agatgtggat aacttgtgta taatatgtta atagactttt gaaaaaagta
    40501 tagttattaa caaaaataac atataaatca acaaaatttg ttgataagta tggcagaaat
    40561 gttaataaaa tgtaaaaaaa gaaaaaacaa aagctattat caaaagagat tcatgttgaa
    40621 ataactaaat ttctaagtaa agaaaacctg tgaatatgtt gattattatg tggataacta
    40681 ttaattcgat atttatttat atagtatgaa taattgaata atatatctaa aaaaagccat
    40741 cctattatta agtaaactta ttgaaaggat ggctttttat gaatagaaaa ttgtgcaaag
    40801 caataatacc tttatttatt tctatttttt gcatgttaat aataagtata aaagctgacc
    40861 aaggatatca aaagaataat gacagagcaa ttactgtcat gaataataat gatactgaag
    40921 atgatgctga aaaaaaacaa gaaatagact atgaaaaaac atgttggaat ttgcaatctt
    40981 tgtttaaaag tgatgaccaa tggaaaaaag aacttaagag ttttgataaa gatactaaag
    41041 agttgaaaaa ttacataggt aaagtaacaa aatcgccaac acatttgtct tttgccttga
    41101 acattaaaga aaaacttgat ataagactaa ataaactttc tgcctatgta aaattaaaac
    41161 aagatactaa taaaaattcg tataagtact tagatatgaa tgatagtatg agtaaatcct
    41221 atgggaatta tatggggatt tgttcagatt tagagttaga aatactaaaa ttatcaaaca
    41281 aagattacaa gaaaattatt tcaaataaaa atataaatag gaaatatggt gcatatttaa
    41341 gagatataag aaggaataaa gtacattatt tggatgataa atcagaagat attctaagca
    41401 aagtatcttc aataagtagt cttccaagcc aagtatatga attatttaga aatatggata
    41461 gaaaaactaa tttgacacca gctcaatatg cgagtgaatt agagtcatca gatagagaaa
    41521 atagaaaaaa agcttatcaa gatgaactta taacttacaa tgataatata aatacaattg
    41581 caggtttaat tactggtcaa gttaaaaaga atatttttta ttctcaggaa agaggttatg
    41641 agtcttcttt agaaatgtac ctggaatctg ataatgtaga tgaaaaagta tacaataatt
    41701 tgataaggac tgtaaatagt aatatggaca gtctacacaa atatatagat ttaagaaaaa
    41761 aagtattaaa attagataag gttcattcat atgatatgtt tgtgcctatg gttaaggctg
    41821 ttgacaataa tattaattat gaaaaggcac aaagtatagt atattcagcg ttgtcacctt
    41881 taggaaaaga atacaatgat gtagtatata aagctttcaa tgaaaaatgg atagatgttt
    41941 attcacatga aaataaagta agtggtggat actgcttatc tgtttatgaa aatcatccat
    42001 atatactttt aaattataat aattcattag gttcagtttc gaccttatca catgagcttg
    42061 gacatgctgt atatgaatat ttaagttcta agaatcagaa ttattttaat tcaaatccgt
    42121 ctatatttac tcacgaagtt gcatctacaa cgaatgaagc attactttac gagatgctta
    42181 taaaagaagc taaaggcaat gatgaaaaag cttattatat aacacagtat ttagacttga
    42241 taaaagatac tctttataca caaactatgt atgctgaatt tgaaaaaact atacacgagt
    42301 tagtagaaaa aggaaaaagt gtaaacgcat tagtattaaa tgatgtttgg ggacaacttc
    42361 ttaaaaaata ttatggacaa agctatgaat tagaccaatt atctaaggtc ggatgggcta
    42421 gaatcccaca tttttataat agtttctatg tctataaata tgctacaggt tgtagtgcag
    42481 gagtaagttt tgcccaagac atcctaaaga atggttcaga taactatatg agctttctta
    42541 aaaagggaag ttcagattat ccaataaact tgttaaaatc aagtggcata aatttaacta
    42601 atacaaaacc catagaaaat acaatcaaaa aatttgatag tttagtagca gaacttgaaa
    42661 agctattata aacattattg taatccacat ggtaaatata tcgaattaat gataaaatat
    42721 actaaaagtt taaagtccta tcaagatagg actttttatt tattaattag tgggagatga
    42781 ttctatgcaa gttaaagtta aaaaggctgt aataccagca gcaggtttag gaactagatt
    42841 tttgccagct acaaaggcac aaccaaaaga gatgttacca atagttgata agccaactct
    42901 acaatacata atagaagaag ctgtggcctc aggaatagag gaaatactaa taataacagg
    42961 tagaaataaa aaatctattg aagaccactt tgataaatct gtagaattgg aacttgattt
    43021 agagaaaaaa ggtaagaaag aattgttgga ggtagtacaa aacatatcta acatgattaa
    43081 catacattat ataagacaaa aagagccaaa agggttggga gatgcaattt attgtgcgag
    43141 gcattttata ggtgatgagc cgtttgcagt aatgttaggg gatgatattg tagataatga
    43201 tgtaccttgt ctaaaacaac taacagatgc atatgaggaa tatagaacta caatattggg
    43261 tgttcaaaag gtaaatcaag aagatactaa taaatatggg ataatagaag caaaaaatat
    43321 agaaggtaga gtttacaagg taaaagacat ggtagaaaag ccagagtcag gaaaagctcc
    43381 ttcaaatatt gctattttag gtagatatat aataactcct gaaatctttg atattcttaa
    43441 ggatttacca ccaggtaaag gtggagaggt acaacttaca gatgctttaa aaatattatc
    43501 taagaaagaa gctatgtatg catacaattt tgaaggcaag agatatgatg ttggggacaa
    43561 gctaggattt ttagaagcta ctgttgactt tgctctaaag aaagaagacc ttaaggaaga
    43621 ttttataaaa tatttgaaac atgtatgtag tgaatttgat aaaaataaca atgtgttata
    43681 caataataca gatgaaaaaa tagatttaat tgaagtcaat gaatctagag aacttgaaat
    43741 tcaaaaataa ttaatgaaag gtgatgtatg gtggcaaaat caagtcagaa aagaattaat
    43801 agaataatag ctgttgggat tgtgggaatc ctcgtaataa cgagtataac agctgttttg
    43861 agtatgattg caactatgta ataagataaa tgacagcagt tgatatacat aaatataaaa
    43921 accatatata acttgatgta gtcaaaaact tacaatattt tagttatata tggtttttag
    43981 aatttataaa tgtaaaaaaa aatttttata atagttgcat attttaaagg aaaatattat
    44041 tataagatat aaatatagtg ataaatttat aaaaacgtgc taagattgga cttgaaatac
    44101 agtaatatag taggtaataa tgttttaaat tttatttatt gaccataata tataaataat
    44161 gatattatta gacttaactg tttaaataaa acttttcttt ttcatgcctt caatttccaa
    44221 aataagtatg tatataaatg aatatataga tgaggaggat ttttttgatt ttagatgata
    44281 aagtattaga aggattaggt agtaagacct tagttgagct acgagagata gctaaagaat
    44341 taaaaataaa atcaattact acatataaga aaaatgagtt aattgagatt ataaattcca
    44401 aaagtaaaaa tgaggaaaaa gtaaacgaag agactaaaga aaaaaatgaa gatattaaag
    44461 aagacataaa gaatagaaaa gaaaatatag agcgtgaaag tatagaaaaa gaagttgaat
    44521 ataagagccc taccaaatca tacaacaaga aagaaataga tacccaaaat atttcccaaa
    44581 accaagaaca tagaaatcaa agaaatgact ataataagac aacgagtgta aatgatagca
    44641 ataagtcatc aaatatatct aatagaatgg tcagaaataa taataaaaac tattatatgc
    44701 ctaaacaagt tgatgaatct aagatagtag atgaatttaa cacatcaaaa gaggatgaag
    44761 tagtaggtgt acttgaaatt ctgccagatg gatttggttt tttaagaggt tcaaattatt
    44821 tgtctactga aggagatgtt tacgtttcac catctcaaat aagaagattt aatatgaaaa
    44881 caggagacaa gattaaggga ataactagac atccaaaaag tggagagaaa tttagagcac
    44941 ttttatatgt tcaaaaaata aatgatgaaa accctgacac agctatacaa agaaatgcgt
    45001 ttgagacatt aacaccaata tttccagaag aacgacttac actggaaact aatagaaatg
    45061 aaatagctac aagaattata gatttaattt cacctatagg aaaaggacaa agaggattga
    45121 tagtagcacc tccaaaagct gggaaaacag ttcttttaaa aagcgttgca aatagcatag
    45181 ctaaaaatca tccaaatgtt gaacttatag tactccttat agatgaaaga ccagaggaag
    45241 ttactgatat gaaagaatct attgaaggag acgttatata ttccactttt gaccaagtat
    45301 caagtcatca tgtcaaggtt gcagagatgg tgttaaatag agctcaaaga ttagttgaac
    45361 atggtaaaga tgtagtaatt ttactagata gtataactag attggcaagg gcatataacc
    45421 ttacaatatc tcctacaggt agaactttat ctggaggtat tgaccctggt gcacttcatg
    45481 gacctaaaaa gttctttggt gcagcaagga atattagaca gggtggttct ttgacaatat
    45541 taggaacagc attggttgaa actggttcta ggatggatga tgtaatattt gaggaattta
    45601 aaggtacagg aaatatggag ttacatttag atagaaaatt agcagaaaaa agaatattcc
    45661 ctgcaataga tatttacaag tctggtacaa gaagagatga tttattgctt gatgatgaag
    45721 aaaagactgc tctttggaga ttaagaagag aaatgagtaa taattcagta atggaaatta
    45781 cagataaagt cattgaactt ataaagagaa ctaaggacaa taaagagttt gtaaaatcaa
    45841 taaagaattt gtaaaatcaa taatacaaat gcaaatatgg attatataaa ctaatagaaa
    45901 aatcacttga atggcataat aagttgtgct ataataatgt agttgataat gtaaaaagaa
    45961 agttaatata attaattttt ttaaataaag agaagaggtg actataatgc aaaaggaaat
    46021 acaaccaaaa tacaatccag ttgaagtgcg ttgtgcttgt gggaacacat ttgttgctgg
    46081 ttcaactaag gacgaaataa aagttgaaat atgttcagag tgccatccat tctatacagg
    46141 aaaacaaaag aacatagaaa aaggtggaag aatcgacaaa ttcaagaaaa gattcaaaat
    46201 ggactaattt ttataaaagt ggagttggag gaagccaact cctattttat tacaaaaaaa
    46261 cattcatatt ctactttaaa ttgtcttttt gtattataat aataaatatg taaaattgag
    46321 tattctaaat acaataaaat gaggtgagtg gatggcaaaa caagctgtag gtgggcaagc
    46381 tgttattgaa ggtgtaatga tgcagtcaaa agacaaaaga gcggtagctg ttagaaaaag
    46441 tgacggagaa atagcactaa aagaagatag aataaaaagt tgggtaagag ataagaatat
    46501 agataagata ccttttgtta gaggttcttt tgttatgata gatacaatga ttcaaggcat
    46561 aaaaagttta aacttttctt cagagttttt tatggaagaa gctgaagagg ataaatttga
    46621 tttatttata aagaaaattt ttaaagataa ggcaaatgat ataattataa tattttcatt
    46681 ggtcatagca atgttacttt ctgctggatt attcattttt atacctacat tggtaggagg
    46741 agctttttct aaagtaatgc ctaatgattt tatgcttaat ttaatagaag gaatcataag
    46801 gatagctata ttatttgcat atatagtatt gatttcacga agtaaggata tagaaagagt
    46861 ctttcaatat catggagcag aacataaatc tatatattgc tatgaaaatg atttagaact
    46921 tacagtagag aatgctagaa aatttaaaag attacatcct agatgtggta caaatttcct
    46981 atttatagtt atggctgtat caataatatt gtttgcattt tttggttggc caaatccaat
    47041 tcttagaata tttatgagaa ttatatgtgt gcctatagta gcaggtttat cttatgaggt
    47101 aattagagtt cttgggaaat atgataatgg atttactaaa attattgcat atccaggaat
    47161 gatgttgcaa tattttacaa caaaagagcc tgatgatgag caattagagg ttgctttgga
    47221 agcattgaaa gcagtagtag attagagaaa aggatatttg ttatgacgat aaaagatata
    47281 attattaaat attcagataa actaaaagat ataagtgata ctccaagatt agatacagag
    47341 ttattgttac aaaaaacatt gggtgttgat agattatata ttcacttaaa tctgaataaa
    47401 gagctaactg aagaacaaaa aacaaaattt atgggttttg cagaagaaag attaaatgga
    47461 agaccaatag catatatagt tgaaaatagg gagtttatgg gattagattt ttttgttaag
    47521 gaaggcgtat tgattccaag accagataca gaaactttag tagaagaaat aattgaaata
    47581 tgtagagaaa aaaaagatgt aagtatatta gatataggaa ctggttctgg agctattaca
    47641 ataagtttag caaagtacat tgaaaattct aaaatcatgt cttttgatat atcagagaca
    47701 gcattagaaa tagctaaaaa aaatgctatt ataaatgaag ttggtgaaaa aataaagtat
    47761 attaattctg atttatttac agcaataagt gatagtaaca taaaattcga tatcatagtt
    47821 tcaaatccac catatattaa aaaacaagat atagaaactc tgcacaaaca agttaaagat
    47881 tatgaaccat ataatgctct tgaaggtgga gaagatggat tagactttta tagaagaata
    47941 acagaacaag gtaagaaata tttaaataag tgtggaatat tagcatatga agttggtcat
    48001 aatcaagctg aagatgtaat aaatattatg aaaagtaatg gatataaaaa aatatataca
    48061 aagaaggata ttcaaggcat tgatagagtt gttataggtt acaatatatg atataatcaa
    48121 caattgatat gttacattta atcgaggtga aagtatgtta aaaaaattag aggtattgga
    48181 agatacatat aaagacttaa gtgaaaaaat aggtgatccg gatgtaataa atgatcaaaa
    48241 agtttggcag aaatatataa aagaacatgc tgacttggaa cctattgtta tgaaatacag
    48301 agagtataaa agcgttttag atagtataaa agaatcaaaa gaaattttac aagaagagtc
    48361 agatgaagaa ttaagagaat tagctaaaat ggagttagct gagatggaag aaaaagtagc
    48421 acctctggag gaagaaataa aaatactatt attgcctaaa gaccctaatg atgataagaa
    48481 cgttatagtt gaaattagag gtggagctgg aggagatgaa gcagctctat ttgcgggtga
    48541 tttatttaga atgtactcaa ggtatgctga aagaagaaga tggaaaatag aattattgag
    48601 tgcaagtgat actggagttg gtggatataa agaagtatct tttatgataa aaggtaaagg
    48661 tgcttattca agattaaagt atgaatctgg agttcataga gttcaaagaa taccatcaac
    48721 tgaatctggt ggaagaatac acacatcaac ttctactgtt gcagtattac cagaagttga
    48781 agatgtagag gttgaaataa atccaaatga tttaagaata gatgttttcc gttcttcagg
    48841 taatggagga caaagtgtca atactactga ctctgctgtt agggtaacac atatacctac
    48901 aggtgaagta gtatcttgtc aagatggaaa atcacaatta aaaaataaag agcaagcttt
    48961 aaaaatatta aaagctagac tttacgataa agcattggca gaacaacaca aagatatagc
    49021 agcagaaaga aaaagccaag tcggaacagg agacaggtca gaaagaataa gaacatataa
    49081 tttccctcaa ggtagaataa gtgaccatag aataaattta actttatata agttagatgc
    49141 attcttagat ggagatatag atgaaatgat agatgcttta attactgttg accaaacaga
    49201 aaaaatgact gcaatataag taatgtttta aagaagctgt ttcttcctag aaattatttt
    49261 ctattttgaa acagctattt tgcattttag gtcatgttat catattctat tgtatataat
    49321 tgttatatac aagtatagga gggttatatg atactaaaag taacaataat tgggcttttg
    49381 gcaggtgtta tagggacagg attaggagga gtaatatcag ccatttttaa aagagaggtc
    49441 gataaatatc taagtttttt tatgggatta tctggtggaa taatgttggc tgtagtggtg
    49501 tttgatttaa tgaaagaatc catggacaaa atggggataa tcaatacagt tatatttacg
    49561 tttgttggag ctctcataac tatgtacata aaaacaaaat tagatgtgtc tggaaatatg
    49621 gcatctggat atcttatatt cataagtata ttactacata atttaccaga aggactagcc
    49681 attggttctt cttttatgtc aactgaaagt ttaggtatta cacttgctat agttataggt
    49741 ctacataata taccagaagg gttagctatg gcattaggct tagtttgcaa taaaatgaaa
    49801 ctaagtaaag ttattttgtt tactgtaata gctggattac ctatgggtct aggaagtttt
    49861 ttaggtgttt attttggagg agtatttact tctcttatag gagtatttct tgctacagca
    49921 ggaggtacta tgatgtatgt tgtattggaa gaaatttttc cacattcaaa aagtgtgtat
    49981 tgtataattg gtttcttgtt aggtacaatg attgtaaatt atatataaaa ttaaaatgaa
    50041 agcaggcata caaaagatga aaactattat aagtaatatt gatataaata atattgatta
    50101 tgaggaaata aaaatacaag caaaacttct tagagaaggt aaaacagtta tttttccaac
    50161 tgagacagta tatgggttgg gtgcaaatgc attagatgaa aatgcagtaa agaagattta
    50221 tgaagctaaa ggaagaccta gtgacaatcc attgatagtc catatatatg aaaaagaaga
    50281 agtatatgac ttagccaaag atataagtga taaagctaaa cttgttatag aaaagctttg
    50341 gccaggacct ataacaatta tattaaataa aaaagatata attccataca aaactagtgg
    50401 agggcttgat acagttgcaa taaggatgcc ttctaatgtt attgccaggg caataataaa
    50461 agaagctggt atacctattg cagctccttc tgcaaatatc tcaggtcgcc catctccaac
    50521 aaaagcaaaa catgtatatg aagagatgaa tggaagagtg gatggaatag ttttaggagg
    50581 agacagtaat tttggattgg agtcaactgt attggattta acagaagaga ctcctatgat
    50641 actgagacca ggaagtataa ctaaagaagt tttagaggaa cttcttggag aagtaaagct
    50701 tgacccatca ttaagtaaaa aagaagataa tcaaaaagct aaagctcctg gaatgaaata
    50761 taaacattat tctccaaatg cagatgtata tataatatct ggaaaaagag aaaatgtagc
    50821 aacaaagatt aatgatatga taaaatctaa tagagacaaa ggtctaaaga caggtgtaat
    50881 gtgtattagt agaaaccgag agttttatga tggagaagta atcgatttag gaaactctct
    50941 tgaagaagtt gcaagtaatc tgtttgatgc tcttatagaa atggacaaaa agggtgttga
    51001 tgtcatatat tcagaagagt tccctaagac tggagttgga caagcaatta tgaatagact
    51061 actgaagtca gcaggataca aagtgataaa ggcttaagag ggagttgtaa aatatgaata
    51121 tacttattgt gtgtacaggt aatacttgta gaagtcctat ggctgaggct atactaagga
    51181 aagcaattaa agaatctgga agaagtattg aagaatattc tatatcatca gcaggtatat
    51241 ctacagcaaa tggaatgggt gcttctgaaa attctataga agtactaaaa gaaatcggta
    51301 tagacttatc aaatcataga agtaaagtaa ttactaaaaa attaattgat gaatcagata
    51361 taatactaac aatgactaaa tctcataaag aaattttagt acaagcagtt ccaaaatgca
    51421 aagaaaaagt gtatactttt aaagggtttg caaataaaaa tgaggaagat atatctgacc
    51481 catttggtgg aaatttggat atttatagaa gcactatgag agaaataatg tattctgtca
    51541 acgaaatagt aaaaaaaata tgaaacggag gataaacaaa tgaagatagg attaggctgt
    51601 gaccatggag gatataattt aaaaaaagaa ataataagct atttagaaag taaaggtata
    51661 gaatgtgtag attacggaac taataatgct acagattctg ttgattatcc tgtttatgga
    51721 gaaatagttg caaattcagt tataaataaa gaagtagatt atggaatatt atgttgtggt
    51781 acagggatag gaatatcttt agctgctaat aaggttcctg ggataagatg tgcagttgta
    51841 tcagatgtat tttctgcaaa aatgtcaaaa gcacataatg atgcaaatat gttatcatta
    51901 ggcgaaagag ttttaggaaa aggtctagca cttgagatag ttgaagcatg gattaatacg
    51961 gattttgaag gtgatagaca tgctagaaga gtaaacatga ttaaatcaat agaagaaaaa
    52021 cataataaat aataaaaatt acagttttat caggaggaat tatcaaaatg agcaaagtgg
    52081 tagagacaaa ccatccatta atacaacata aattaacttt aatgagagat aaaaatactg
    52141 gttctaaaga ctttagagag cttttaactg aaattgcaat gttaatgggg tatgaaataa
    52201 caaaagatat accattaaaa gatgtagaaa tagaaactcc tattcaaaaa acatcatcta
    52261 aagtagttgc aggtaaaaaa ttagcaataa ttccaatttt aagagcaggt cttggaatgg
    52321 tagatggatt agtgagctta atgccagcag caaaagttgg acatgtagga ctatatagag
    52381 acccagaaac attaaaacca gttgaatatt attgtaagct tcctcaagac ataggagaaa
    52441 gagatataat agtagttgac cctatgcttg caacaggggg ttctgctgta gcagctatag
    52501 atttacttaa gtctaaagga gctaaaagta taaaattagc taatttagta gctgcaccag
    52561 aaggtatagc agaagtgcaa aaatatcatg atgatgttga tatatatgtt gcatcagttg
    52621 acgaaagatt aaatgaacat ggatatataa ttcctggtct tggtgatgct ggagatagat
    52681 tgtttggaac taaataaaaa taaataacta taaatattta ttagggactt tcttatatag
    52741 tgtatataat tttatgtaag aaggttcttt tttatatatt ttttctcaaa ataggaatta
    52801 ttgaattgat aaatttctcg aattgagaat aaaaagtttt taccaattaa aaaagattgc
    52861 ttattttaca aggttttata gaatagtagt ctattttaat gtggcatgtt agttgcttac
    52921 gattaagatt ataggtatta agtgtattag ttaaattgag aattgaatat taataatatt
    52981 attgggagtg gtaatacatg aagaattgta tagtagtaag aggggctgga gatttagcaa
    53041 cagcagttat tcataaatta cacaacagtg gttttaaagt agttgcgtta gaaatagaag
    53101 agccattagc tataagaagg caaatttctt tttgtgaggc tgtttatgaa aaaaaagtaa
    53161 ctgttgaagg tgtagaatgt atattttgca atactgaaaa agaaatagaa gaagttttat
    53221 ctcaacataa agtagccttg attatagacc caaaaggaga aaaattatct aaattaaatc
    53281 caatcgtttt ggttgatgga atactcgcta agaaaaactt aggtacaact aaagatatgg
    53341 caccatttac aatagcttta ggtcctggat tttgtgcagg agaagatgta gatgttgtta
    53401 tagagacaat gagaggtcat aatctaggaa gaataataac taatggtaga gcagaaaaaa
    53461 atacaggagt accaggagca ataaaaggtt tttctaaaga gagagttatg cattcacagt
    53521 atgatggcat aatggaaaat gtatgtaaga taggtgatat agttgaaaag ggagacctac
    53581 tttgctatat taataattat gaaaaaaaac atgaagttag agcaacaata tctggagtcc
    53641 ttagaggttt attaaaggat ggagcaaagg taaacaaaaa tttaaaaata ctagacatag
    53701 accctagaaa agaagaagtt aagaatgctt ttacaatatc agataaagct agatgtatag
    53761 ctggaggggt tttagaagcc atactaaaca atgggattta tccccaaaat taatttatat
    53821 tagataaagc tattaatatt ttattaagat gatataataa atttaataga ttaaagagag
    53881 catatgacgg gtatttaaaa tttaaatggt aaaaatgctc tttttttatt ggacattcaa
    53941 ttaacatgtg tatataatat aaaaataagt atttatttag gttgaaatct aataaatata
    54001 taataccagt acataagcaa agaggagaaa agtaaattat gagaccatca tgggatgaat
    54061 acttcatgga aattgcagaa gtagtgaaaa agcgttcaac ttgtattaga agacaagtag
    54121 gagctgtaat tgtaagagac aaacaaatat taacaactgg atataatggt tctccgagaa
    54181 atcttgaaca ctgtgaaaat attggatgta agaggcagga attaaatatt ccctcaggag
    54241 aaaggcatga attatgcaga gcattacatg ctgaacaaaa tgcgataata caagctgctc
    54301 ataatggtat tagtgtagat ggggcaactt tgtatgttac tacaagacct tgtgttttgt
    54361 gtgcaaaaat gtgcataaat gcaggtatat tgaaaattgt ttatgaaggt gattatccag
    54421 atgatatgtc tacagaatta ttaaaagaag caggaataga actcataaaa ttttagtgga
    54481 taaaatttat aagacaataa atgtcaaatt atgacactga aatacaatga tatcaaatga
    54541 ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa gatatatttt ataattaaat
    54601 aaatttatga gatagattaa ttacatgtta tttattttat aataatgagg gatactattt
    54661 atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt ttagtttaat aagtcaaata
    54721 ggattgatga ttgtaatatc aatattagga tgtactttct taggaaaatt tttagatagt
    54781 aaattgaata ctactccagt tctgacaata atatttttat tgttgggtgt tggaggtgct
    54841 tttagtggtg tttataaaac tctaatagta tatacacgaa ggaagtgata tattggatat
    54901 aaaattactt aaccaggtaa ggtcagtaac aaaaggtata gtaatatttg atgtagtttt
    54961 aattgtgcta atgttgataa catcaacatt aagtaaacca gtattattag gacttatatt
    55021 tggctctata atagctatgt taaattttag attattggcg atatcactgg aaaaatcagt
    55081 gacatttcct gctgggaagg cacaagctta tgcatcagca caatatgctg ttagattatt
    55141 tataatggca gttgttctaa ttgcatcagc tacagtacca catttaaata taatcggaac
    55201 agcattggga ttattgagca caaagtttgt catattatca aaaaacttta taaataaatt
    55261 aaagaggaag gaggcataag tatgaatcaa gcgcattatt atctcatagg aaactttaag
    55321 attagtgaaa caatactagt aagttgggga ataatggcct ttttagcgat agcatcttat
    55381 tttttgacta gaaacttaaa aaaagttcca actagtaaag ttcaaatatt cttagaattt
    55441 gcagtaggtg gtcttgctaa aatggttaca gatgttatgg gtaaaaatac agtaaaaaga
    55501 atgccatata tgataccata cataggaagc ttgtttttat ttttcgcatg ttcaaattta
    55561 agcccattac ttggattaag gtcaccaaca accgacttag acacaactgc tgcatgggca
    55621 atgattacat tttttatgat ttattttgca ggagttaaat ttaaggggtt atcatacttt
    55681 aaagaattgg ctcaaccact tgccataatg ttaccaatca atattatagg tgagttagct
    55741 agaccaatat cattaagttt ccgtccattt gggaatattt taggtggaac aataataatg
    55801 ggtctgtttt atcaactttt aggttttata tctagtttaa taccaggatt atcaatacca
    55861 attggagaaa tattaatacc agtcccatta cacttgtact ttgacttatt tgcaggattg
    55921 ttacaagcat atatatttat aatgttaaca atggtattta tatcaagtgc aggagagtta
    55981 gattaagaaa tcaacttata ttaagataaa aataaaataa tttaaaaaat taaagtttta
    56041 aggaggaaat aattatggaa acagcaatag tagcggcagc atcagcaata ggagcaggga
    56101 tagcagttgc aacaggtata ggtgcaggta taggtcaagg tatagcagca gctaaagctg
    56161 cagaagcagt aggaaaccag ccagaagcta aaggtgatat aacatcaact ttattactag
    56221 gagttgctat agcagagtct tcagcaatat atggtttggt tatatcaata atacttctat
    56281 tcgtaaatcc attctttaaa tacttaggaa tgtagattta gtccttaaat attttgagat
    56341 aggtttatag gtaaatcatc tcaaacttat aatcatgaga tgaactttat catctcgtga
    56401 ttataagtag ttatgattta tggaaaggag gataattatg gaaaaagcac tagtaggtat
    56461 aacgtgggaa tttgtgttcc aaattgttaa tacttttata atatttttac ttctgagaaa
    56521 gcttttattc aagccagtat taaatataat tgaatcaaga gaaaatgata ttaaaagtga
    56581 tttggcagaa ggcgaaaaag caaaaaatga aggtttagct ctgaaaaaag aatatgagtc
    56641 aaaaatcaat tttgccaaag atgaaggtca agaaatcata aaacaagcaa ctataagagc
    56701 agagcagaaa tcagatgata tagtaaatac tgcaaagaaa gatgctcttg atataaaaga
    56761 aaaagcaaac aaagatatag aacaagaaag acaaaaggtt ataaatgaaa tcaaaaatga
    56821 tatatctaac atagcactac ttgcagcatc taaagttatt gaaaaagatt tagataaatc
    56881 taaacatgaa gaattgatag aaaactttat aaaagaggta ggggaagcta aatgataaat
    56941 gtaatagcaa atagatatgc agaagcccta tttcaattag gagaagaaga aaattcaact
    57001 gatgtcttat tcaaagaact agaaaaagta gtagatatga tgactaaagt tagtaaagat
    57061 ttctacaaag ttttaaaatc accattagtt tcaaaatcag aaaagaaaaa cttggtagaa
    57121 attattttta gtaaagaagt aagttctaac ataaaaaact ttttaaaagt tttagtagat
    57181 aaagatagaa tatcatattt agaagatatc gaattagctt ataaagaatt attaaataag
    57241 aaaaacaatg taattgatgg agtagcaata agtgctattc caatgagtga aacagatatt
    57301 aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa atgttactat agaaaatgtt
    57361 gttgacaaga ctatactagg gggagttcta gttagaattg gaaatgaaca aatagatggc
    57421 actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat ctgaagtaat atcttaagaa
    57481 tggcggtgaa cccatgaact taaaacctga agaaataagt tctataatta aacagcaaat
    57541 aaaaaattat gagaataaag ttgagttgac agatacaggt agtgttttaa cagttgggga
    57601 tggtatagcg agtgtatatg gattagaaaa agctatgtct ggtgagttgc tagagttccc
    57661 aggtgaaata tatggtatgg cacttaactt ggaagaagaa gtagttggag ctgttatcct
    57721 tggagatgac agtgaaataa aagaaggcga tatagttaag agaactggaa gaatagttga
    57781 agttccagtt ggagaagctt taataggtag agttgtaaac tcactaggtc agccaataga
    57841 tggaaaagga cctatagcgt acacaaaaac tagaccagtt gaatctgagg cacctggtat
    57901 aatagataga aggtctgttt atgaaccatt acaaacagga atcaaatcaa tagattcaat
    57961 gataccaatt ggtagaggtc aaagagagtt aataataggt gacagacaaa ctggtaaaac
    58021 atctatagtt attgacacta tactaaatca aaaaggaaaa gatgttatct gtatatatgt
    58081 agcaattgga caaaaacgtt ctactatagc tcaattagtt agctctttag aaaaaggtgg
    58141 agctttggat tatacaatag tagtttcagc aacagcatct gaatcagcac cacttcaata
    58201 tatagcacca tatgctggag cagctatggg tgaagaattt atgtacaatg ggaaacatgt
    58261 acttatagtg tatgatgatt taagtaaaca agctgttgca tatagagaaa tgtcattgtt
    58321 acttagaaga ccaccaggac gtgaggcata tcctggagat gtattctatt tacattcaag
    58381 attacttgaa agagcagcta agttatctga tgaattgggt ggaggttcaa tgactgcctt
    58441 gcctataata gaaactcaag ctggtgacgt ttctgcatat ataccaacaa atgttatatc
    58501 tataacagat ggtcaaatat acttacaacc agagttattc tattcaggtg taagaccagc
    58561 agttgaccct ggtatatcag tatcaagggt tggtggttct gcgcaaatta aagcaatgaa
    58621 aaaagttgca ggaacattaa aacttgcata ttcacaatat agagaacttg cagcattctc
    58681 acagtttggt tctgacttag atgaagatac taaaaagaga cttgctcaag gtgaaagaat
    58741 cgttgagata ttaaaacaag gtgagcatca accaataaaa gttgaaaatc aagttatgat
    58801 aatatatgct gttataaata atcatttaga ggatattccg atagataata tagctagatt
    58861 tgaatcagaa ttgtatgcat ttgtagataa taattatccg gaaatatcaa gaaaaatatt
    58921 aggtggagaa gacttcactc atgatttaac agatgcaata aatgaattta aagaaaagtt
    58981 tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat taattggcag gagctggaat
    59041 caaagcaatc aaaacacgta tgggaactgt tgaaagtaca aagcaaataa ctaaagcaat
    59101 ggaattagtt gcatcttcta agcttagaaa agcaaaagaa aaggctgaag attcaagaga
    59161 atattttaat actttataca atacagtaaa agatattgcc caaacaacac gtggaattag
    59221 aaatgaattt ttaaaagaaa gagaagtaaa aaataaatgc tatgtagtag tagctggaga
    59281 cagaggactt gcaggtggat acaattctaa tgtattaaag gaatctgtct ctcatatgaa
    59341 aggtaaaaag gaaacagtga ttactgttgg aaagaaagca catgaatttt tttctaaaag
    59401 aaactacaat atagcaaaag atataaatag tgttgaagct tgtggatatg aagatgcgac
    59461 agaaatttca caaactatta ttgacctgta taaaaaaggt gaagtagatg aagtttatat
    59521 tgcttataca aaatttaaat ctgctttatc acaagaagtt gaaattttga aattgcttcc
    59581 attagtcttt gaaaaagata cagacgaaca aggaatggca gtaaaagaag aaaaacaaaa
    59641 tagatcaaaa gtacaatata taccatcagc agaagcagtt ttaagttata ttgtacctca
    59701 gtttatatca ggtacagtat atggtggaat tttggaatct tttgcatcag agcaaggtgc
    59761 tagaagaact gcaatggaat cagccacaga caatgcgaat gaaatgctat ctaatttaga
    59821 gttaagttat aacagagcaa gacagtcagc tgtaactcaa gaaataacag agatagttgg
    59881 tggagttgaa gctctaaaat agggaggtta ggaaatggca aatgtaggta aagtagtaca
    59941 gattgtagga gctgtactag atgttaagtt tgatagtgaa caaagcctac ctaacttatt
    60001 aaatgcgtta gtaataaagt taggagataa agaaatagtt gcagaagttg ctcaacatat
    60061 aggtgatgat actgtaagat gtatagctat gagtgcaaca gacggacttg taagaggtat
    60121 ggaagttgta gatacaggag ggccaataag tgttcctgtt ggagatgaaa ctttaggtag
    60181 aatattcaat gttcttggaa aaccagttga tgggaaacca gctccaaaat ctgcacctaa
    60241 attacctata catagaccag caccggcgta tgatgaacta gaaactacag cagagatact
    60301 agaaactggt attaaggtag ttgacttact agctccatat ctaaaaggtg gaaaaatagg
    60361 tctatttgga ggagcaggag taggaaagac agttctaata caagagctta taaacaatat
    60421 tgctaagcaa catggtggta tttctgtatt ttcaggagta ggagaaagaa caagagaagg
    60481 taacgacctt tatggcgaaa tgagtgagtc tggagttata aataaaacag ctctagtatt
    60541 tggtcaaatg aatgaaccac ctggagcgag aatgagagtt gctttaactg gacttacaat
    60601 ggcagaacat tttagagatg agcaaggaca agacgtttta cttttcgttg ataatatatt
    60661 ccgtttcaca caagctggtt cagaagtttc agcacttcta ggacgtatgc catcagctgt
    60721 tggttatcag ccaacattag ctactgaaat gggtgcactt caagagagaa taacatcaac
    60781 taagaaaggt tcaataacat ctgttcaagc agtatatgta ccagcagatg acttaactga
    60841 cccagcacca gctacaacat tctctcactt agatgcaaaa acagttttat caagacaaat
    60901 atcatcactg ggtatatatc cagcagttga ccctcttgaa tctacatcaa ggattcttga
    60961 ccctagtata gtaggaaaag aacattatga agtagctaga ggtgtgcaat caatacttca
    61021 aagatataaa gaacttcaag atataattgc tatacttggt atggatgaat tatctgatga
    61081 agataagtta atagttgcac gtgctagaaa gatacaaaga ttcttatctc aatcattcac
    61141 agttgctgag caatttacag gaaatccagg tcaatatgtt cctgttaaag agactgtaag
    61201 aggatttaaa gaaattcttg aagggaaaca tgatgactta ccagagtcag cattcttatt
    61261 tgtaggaaca atagaagacg ctgttagaaa agcgaaggga agtatgtagt catggccagt
    61321 gaattttcat tgaaagttgt aactccaagt aatacttttt atgatggaga tgttgaaatg
    61381 atagtagtta gaactacaaa aggagacaaa ggggtgttga aaggtcatat accatttgtt
    61441 tcaggtatac ctgtaggaac tttaaagata aaaaaagagg gaagttttaa agaggcgaaa
    61501 atagcagaag gattcattaa tgtaactcaa gataaaactg tcatattgac agaatctgct
    61561 gagtggattt aataaattaa tttagagtat aaaaagtcat tttgaccaaa aaaaactttt
    61621 gggtaaaatg gcttttttgc gttttggtgt taataaaata acgataaaat tgtaagtttt
    61681 tagtgataat aaattcacaa aatgtttaaa tttactgcca aattatgttg taattaataa
    61741 gataaagaag atttagatag cttttatgta agtgaaaaag tcttcatata agaaatacat
    61801 ataaaatttt tataaaattt aaaaaatttg cttagttgtg tagtataa
//
LOCUS       DAENCV010000006        60192 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAENCV010000006 DAENCV010000000
VERSION     DAENCV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.006"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..587)
                     /locus_tag="KQD82_000326"
     CDS             complement(<1..587)
                     /locus_tag="KQD82_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378831.1"
                     /translation="MKKITKKEIKKYVREAISNNFNWEIDRCGFYIKNDSIKFFISYK
                     GQGADENIYNNTYEEIIYIDDIIEDYKRKEYNLKDVDSIVHENVNNMIIDYNEEIEEN
                     ERCIRSFIKELKSSVGEEFTVLEYDNFVQEKYNYLVNKTDSWDFFMEGEVYNYLDCGS
                     YTYSGLGKDYDVDINIVFDVIKINSEEDYKSIIKI"
     gene            complement(571..729)
                     /locus_tag="KQD82_000327"
     CDS             complement(571..729)
                     /locus_tag="KQD82_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378832.1"
                     /translation="MIRYMKDINNISLEDADMLAKEGFCFIIKDGKLRGFKIEKIKIG
                     GYENEKNN"
     gene            complement(726..1223)
                     /locus_tag="KQD82_000328"
     CDS             complement(726..1223)
                     /locus_tag="KQD82_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378833.1"
                     /translation="MKFKNIAMGMLLGLIIVGVAGATKVKAAELDNFYVETTDRVVEI
                     LEDESVVLYDTKEQVYNFYPVCLGDWNYSFKNKKDLDRAVATYKELSNNISHSKDVYV
                     INKLNNNGNIKVFLSDGSSIVYIKNDNKYYFYPSCMGDWYLTLDSKIDLSNCVGTYFD
                     VEVAI"
     gene            complement(1542..2126)
                     /locus_tag="KQD82_000329"
     CDS             complement(1542..2126)
                     /locus_tag="KQD82_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378834.1"
                     /translation="MNEYILISYTKGKGACDHCGRTIKNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCMEYYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDMDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            complement(2152..2352)
                     /locus_tag="KQD82_000330"
     CDS             complement(2152..2352)
                     /locus_tag="KQD82_000330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378835.1"
                     /translation="MIISAWQNSDSKKVLIEVKKDKKNIDVLIAYTNKIKGRDMTMVQ
                     SWILEEVYKTKNNGYGIYELID"
     gene            complement(2406..2768)
                     /locus_tag="KQD82_000331"
     CDS             complement(2406..2768)
                     /locus_tag="KQD82_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378836.1"
                     /translation="MNKYLIGCIGNCLRSMKAIKTRFFLIDNNNIEDVTLKLCKGVEH
                     IAPFRYKYGLANYSFGCGLSHYLDTYNLNTVNGLDLNNTHLIMLTLNDLKNIVGCKTV
                     FDIDVEEKLLNYIKSYYR"
     gene            complement(2806..3309)
                     /locus_tag="KQD82_000332"
     CDS             complement(2806..3309)
                     /locus_tag="KQD82_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378837.1"
                     /translation="MNKIIVFYDIEGYPVFQILVRNVKKYTRQNRIFKLVDSFIKKNI
                     NNINLNDGKIYDSSSWIIFTISKTGHYKLKLNLDIDYDILIKKLDIKKSKRESNSKTE
                     RELFYIGTGWCKRKEIYNLLTRQNQIGYEDYDTLTIDGDVYIRTYNEKCRTILLHSNY
                     QKILKEI"
     gene            complement(3344..3580)
                     /locus_tag="KQD82_000333"
     CDS             complement(3344..3580)
                     /locus_tag="KQD82_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378838.1"
                     /translation="MINYSKYMTATDLINICDILEGETIGEAGTYNSLSTRLGNLIGL
                     DWALKGEELRQQLIQNINNMMYLDNENKIQYKEF"
     gene            complement(3625..3846)
                     /locus_tag="KQD82_000334"
     CDS             complement(3625..3846)
                     /locus_tag="KQD82_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378839.1"
                     /translation="MKINSKISNVVWDIKTKELNKQDILFIENFNPTIYHNDVDCYLW
                     SLDEKLPSGVWLENIKFNNEITEAKAIVL"
     gene            complement(3863..4003)
                     /locus_tag="KQD82_000335"
     CDS             complement(3863..4003)
                     /locus_tag="KQD82_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378840.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIILKCGNSNQLKLLKQSN
                     KL"
     gene            complement(4071..4784)
                     /locus_tag="KQD82_000336"
     CDS             complement(4071..4784)
                     /locus_tag="KQD82_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG4378841.1"
                     /translation="MLNIYITNLGKYNEGELIGEWAELPVNEEELQEILDRIGINEEY
                     EEYFITDFETDIEGLEISEHSNIEQLNELAMKLERLEEYEIEELKALLERGYIDFYDM
                     LEGDICRLSGNYTFIQLDDRSPLDDENLGRSYIEEICCGDLSCIENIAYYFDIEAFSR
                     DLRFDRDMIIENLYEEDIEYYENMTDIEFAENYIERLGDIEELGQETLERYFNYELYG
                     TDLRYENICIASNYLAIVS"
     gene            complement(4905..5093)
                     /locus_tag="KQD82_000337"
     CDS             complement(4905..5093)
                     /locus_tag="KQD82_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378842.1"
                     /translation="MYEQRKAKVIFNKGAGNSRGESYTNRVTIPTTWIKHMDITKLDR
                     EVLLTFDGNKIVIEKIDE"
     gene            complement(5188..5421)
                     /locus_tag="KQD82_000338"
     CDS             complement(5188..5421)
                     /locus_tag="KQD82_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378843.1"
                     /translation="MDEKTLELLREIQESIKILNDKLDEFDYTQDKIKSNVEGLLECF
                     SRIDFRIEDLENGQTSLYTKLDIVQNETAKSIK"
     gene            complement(5433..5756)
                     /locus_tag="KQD82_000339"
     CDS             complement(5433..5756)
                     /locus_tag="KQD82_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378844.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDSMDGRFDGIENQL
                     SDLKEGQEEIKKKLDLTYNQVARNMEGITEVGEKIDTLKNDMNFVEMATSKNWNEIAK
                     LKSIK"
     gene            6503..6820
                     /locus_tag="KQD82_000340"
     CDS             6503..6820
                     /locus_tag="KQD82_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378845.1"
                     /translation="MISDRLRELRKEKGYTIKELAQIINVSDISISRYENNKRDADIK
                     TLKELAKLYDVSLDYITCLTDNRENIQLEEEEVKLLDNYRELDTKSKTIVQEQINTLK
                     KLL"
     gene            complement(7034..7225)
                     /locus_tag="KQD82_000341"
     CDS             complement(7034..7225)
                     /locus_tag="KQD82_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378846.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(7478..7636)
                     /locus_tag="KQD82_000342"
     CDS             complement(7478..7636)
                     /locus_tag="KQD82_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378847.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHILISLCSCIGWLL
                     ILIYIIKH"
     gene            8132..8320
                     /locus_tag="KQD82_000343"
     CDS             8132..8320
                     /locus_tag="KQD82_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378848.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            8742..9656
                     /locus_tag="KQD82_000344"
     CDS             8742..9656
                     /locus_tag="KQD82_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBG4378849.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFESVSYFVGSLAEKESITPIRNSDDSKVSFTMRILVAAALNEIAVADE
                     VKLMMGVPYKSFRKTTLKEVVDTYKGKTFKVKDKIKGGHKEIKISDISIFREGDAALF
                     HTLEGKVNEDKAVGMVSIGFRSTEMSFFEKGFVFNDKLSDTLEAGNQDALTMVQKQLK
                     DRGIIRELNEIDSSNDYDELKKVAYIMASESTAQRISSKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMATAKGLFEVGMEQF"
     gene            9669..9941
                     /locus_tag="KQD82_000345"
     CDS             9669..9941
                     /locus_tag="KQD82_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378850.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTNVTEEKKDIKRENPIIKNIFNNMPD"
     gene            10213..11076
                     /locus_tag="KQD82_000346"
     CDS             10213..11076
                     /locus_tag="KQD82_000346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378851.1"
                     /translation="MKRGEVLEIGKRKKSFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            11254..11502
                     /locus_tag="KQD82_000347"
     CDS             11254..11502
                     /locus_tag="KQD82_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378852.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            11505..12947
                     /locus_tag="KQD82_000348"
     CDS             11505..12947
                     /locus_tag="KQD82_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378853.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDSIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFDRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLKIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            13081..13359
                     /locus_tag="KQD82_000349"
     CDS             13081..13359
                     /locus_tag="KQD82_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG4378854.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            13927..14017
                     /locus_tag="KQD82_000350"
     tRNA            13927..14017
                     /locus_tag="KQD82_000350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13960..13962,aa:Ser,seq:gct)
     gene            14073..14690
                     /locus_tag="KQD82_000351"
     CDS             14073..14690
                     /locus_tag="KQD82_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378855.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCISNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSRYQQIEDLIYNYNALDKAINISNAITNVQIGNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLK"
     gene            15327..16526
                     /locus_tag="KQD82_000352"
     CDS             15327..16526
                     /locus_tag="KQD82_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378856.1"
                     /translation="MEDILKKKEDESLIDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKVQDNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRIDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSEIFKIRIQYLKDKIIEYSKLHKVKRLHVMLLGDLISGYIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIVYSIGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKNINNVIFQKNIYDNDIIVAKICGNTCFGVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKL"
     gene            16657..17556
                     /locus_tag="KQD82_000353"
     CDS             16657..17556
                     /locus_tag="KQD82_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378857.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRLPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELADKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYEEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNQPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILEGNKNED"
     gene            17546..19294
                     /locus_tag="KQD82_000354"
     CDS             17546..19294
                     /locus_tag="KQD82_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378858.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            19311..20789
                     /locus_tag="KQD82_000355"
     CDS             19311..20789
                     /locus_tag="KQD82_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378859.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            20792..20983
                     /locus_tag="KQD82_000356"
     CDS             20792..20983
                     /locus_tag="KQD82_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378860.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            20990..22423
                     /locus_tag="KQD82_000357"
     CDS             20990..22423
                     /locus_tag="KQD82_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378861.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FKLAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            22438..22935
                     /locus_tag="KQD82_000358"
     CDS             22438..22935
                     /locus_tag="KQD82_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378862.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYVVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            23023..24033
                     /locus_tag="KQD82_000359"
     CDS             23023..24033
                     /locus_tag="KQD82_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378863.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGVQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            24087..24719
                     /locus_tag="KQD82_000360"
     CDS             24087..24719
                     /locus_tag="KQD82_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378864.1"
                     /translation="MARAKKENISKTEVKANTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEYIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGMEDLYLFS"
     gene            24734..25363
                     /locus_tag="KQD82_000361"
     CDS             24734..25363
                     /locus_tag="KQD82_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378865.1"
                     /translation="MGTPLVKIYKKFLDGINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGGEIIPAAQLSYTSNYSNKNAEITLMGKDTKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLDEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            25365..26354
                     /locus_tag="KQD82_000362"
     CDS             25365..26354
                     /locus_tag="KQD82_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378866.1"
                     /translation="MSYLSDTKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEVP
                     ITDIDEICITEDTKRAIVAINDITNNDKRALDEKNLLVESDLDVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTINNVAWNDLSENDSVIIPFSKIIGDGFINLGEENEYRIDHSKGVKWLLD
                     KQYRYCNIINQDEKKCVIQANILAKYSGLEVLLLAKDKETNETIDTKKITLRG"
     gene            26369..26863
                     /locus_tag="KQD82_000363"
     CDS             26369..26863
                     /locus_tag="KQD82_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378867.1"
                     /translation="MAFPNKMISNIGTTLMSNQDFAKFMIYNDESQKDILSMPDVKNP
                     VKELKNKKVFLNRRVEKVLKDADISVFIIMAEYRPCTTGSREIKKTKIEIGVVCHDEC
                     QNTANGLRDVALVCCIVDIITQNEEIAGIGKIKLENVYQMYNLNTDYNGFVITVSSES
                     FGDM"
     gene            26863..27666
                     /locus_tag="KQD82_000364"
     CDS             26863..27666
                     /locus_tag="KQD82_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378868.1"
                     /translation="MLENYYITGLPIKLSETLGTVYQPIIEELIKFDMANLEIVNPFL
                     VLEKSYSQLCNEESFELKCKYDAIPILDLMMLTSRKDSSEEIELLSDKIKKSLSILYK
                     TDIKNIEYMNKIKIGILIKFDDKKKNAFISREDFGLVSDLILEMFCIDKKNLFKDDED
                     KWIENTGSEREKQLIAHFKEKERKKREKEAYHLCDYINVVQNIDGYVPIDVILKMTYW
                     QLINVYKTKIQFKNYNESLGYAWSFKYQTDMDKMKHWSKEIKIPLSTVK"
     gene            27711..28529
                     /locus_tag="KQD82_000365"
     CDS             27711..28529
                     /locus_tag="KQD82_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378869.1"
                     /translation="MKRFAVKDAGNVIVKNKVTGEVLFYSQDLNAFNFKMDSESVYAK
                     AKGANTIAFDGAITASLTMEQEVIQMAQLAMLLSSDIDEKTAKVGKRKVLTSDSTKKV
                     TLENIKPVANSISVYSIESDGISIIKKLQFTSSVTGANTEITISTADFNAGDKVAVFY
                     LEEIPKAKVVKIKEESTAPNYVVEAEVMVKTVDGEYMVLYMSVPNAKAQRSIELNLTA
                     ENPSGFNMTLDVLPDENKEYATFTFIGDENLNPARMASMLGVELEDEKDTKPKK"
     gene            28628..29413
                     /locus_tag="KQD82_000366"
     CDS             28628..29413
                     /locus_tag="KQD82_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378870.1"
                     /translation="MVFLDDKFLFDNVSSESMNIKLVTLGDDEILNEYGLPYEEAIKS
                     DSSFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQREFKPFIS
                     YDNIEEIYYFKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTREIKLNN
                     VSNVDEEYAPVIDIECLKEGDITIRNSTISDNEEDNLVISGLELNEKITIDNLYYTVL
                     NSNGENRFNIINRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            29427..29885
                     /locus_tag="KQD82_000367"
     CDS             29427..29885
                     /locus_tag="KQD82_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378871.1"
                     /translation="MNKIQVDKLIQDEVRAIIPIIDENGKEEYIEVRNPDKETKEEIL
                     NKIWVGMENPDLALSQEDILKMLIDKLTNIELNIEIEDLVDSNISSELETVMYHICQI
                     GNELTASLLMNTDVKLGQMKNEILQDRVLKEIEETEEIEKMNNIKDKVVN"
     gene            29886..30326
                     /locus_tag="KQD82_000368"
     CDS             29886..30326
                     /locus_tag="KQD82_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378872.1"
                     /translation="MVFKSLDELVAYTKNKIAASMPEVGKEMKEIMKEEVNKQVYSGY
                     TPVGEWNPNVWGGRTGQLLDAIDVTELSSNSVTSEIQNNGNWVDAFTRNSAFPMERLE
                     KGKVWGVGGYRPQTNIMEESNSKAQERVPKCFKSKMNELGVPVK"
     gene            31512..32312
                     /locus_tag="KQD82_000369"
     CDS             31512..32312
                     /locus_tag="KQD82_000369"
                     /inference="COORDINATES: protein motif:HMM:NF024098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG4378873.1"
                     /translation="MGKIYNLYLDESETHDEKDGNWINKVFCIAGIIVEDKYDCNELT
                     KDLNKIKYQIWKDNINYEALILHEVEIKQANNGYDKKNQNLVKPHNRIFRKNQNVKIL
                     YRGLEKIIRNKKITTLAVCIKHDELDKLYNKEIQNDRTLMALQILIENYVHFLKNNDS
                     KGRIIYEHNGDSNSKIMKMKFNMIKTMGTLFINPYAIQERLLDIEFPSKDENIAGLQI
                     ADFIPNIIARKVSNKKLNKYNIYNTIKRFSYDGNVKNKDKFGIKIIKL"
     gene            32757..32996
                     /locus_tag="KQD82_000370"
     CDS             32757..32996
                     /locus_tag="KQD82_000370"
                     /inference="COORDINATES: protein motif:HMM:NF024637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4378874.1"
                     /translation="MSEDENKVSQENDSTKFCISCGEKLLEGQLFCSKCGKKVDEKTV
                     ISKKASINKNCWYNIDGHSGDFHQYQYQGKKTTRS"
     gene            33010..33843
                     /locus_tag="KQD82_000371"
     CDS             33010..33843
                     /locus_tag="KQD82_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378875.1"
                     /translation="MTIKDFQEKSYVAGANLEDIADTIQTYWKENIYEKKHGSNIDAA
                     IFAAMLDKSDEIEKSKKYDDELLKIYSKLKSVPEGSEDLNDILSTIEKFYNSYTDFHS
                     FATEPSGSYVDYSASNNTKTDEFLSNYRALENSIKSNSELNKAKSTSETMMMNIEKAM
                     KEYEIISDSIPKDIDPLDENLYEKVKKIVPDFYSDKNIDVSYIENNKGKFKNDERVSK
                     ALDHFSAAKTSYDNMVKYAKAYKKSNSQDDLEKFKNEIFDFRAYVAIYGLQVEYSDEE
                     N"
     gene            complement(33979..34428)
                     /locus_tag="KQD82_000372"
     CDS             complement(33979..34428)
                     /locus_tag="KQD82_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4378876.1"
                     /translation="MIGEKLKHLRIKNNLKQSDLARILDVTQGAVGMYENDKRTPSPE
                     LIVKLAEYFNVTTDYLLGHTKTNYSVSANIPGMPSIICEDTSIYDIVDEKKDIKSLED
                     INKFLENNNYNDEVKEVLKKYIQLDEMDRKAIDRMIDNAYERLKEDN"
     gene            34731..34907
                     /locus_tag="KQD82_000373"
     CDS             34731..34907
                     /locus_tag="KQD82_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378877.1"
                     /translation="MTPRTGRPPSDNPKKNEYRIRMSDDELEILNHCCKELKLTKADV
                     VRLGIKKVYDELEK"
     gene            35030..36106
                     /locus_tag="KQD82_000374"
     CDS             35030..36106
                     /locus_tag="KQD82_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG4378878.1"
                     /translation="MGIINRENVNEIMSFEESELSKKFEGMDVEIIELNGELLFELYS
                     TGAALGYTRNNPSGGKRYLQIRRDRIDNVIEKAEIKPLDRSGTKYLTEPQLYDFMLEA
                     KTAKCRPFKKWVTNEVLPTIRKHGAYMTNEALEKAINDPDWTIQLLTELKKERATKEK
                     LRVEQEKNKPKLELANAIESSSSSILIAQLSKILNQNGVDIGQNRLFEWMRNNEYLIR
                     KKRADHNTPTQKSMDLKVLEVSESTGIDKDGNTVIRQTPVVTGKGQIYFVNKILKENN
                     KVLKRFMINNDYIRGVMVEIGKTYAIYDHEYYGFIGILKSVRNEKIRVEKIGDVSSSR
                     DIPICMIDYIEEASKEDINKYLSK"
     gene            36207..37367
                     /locus_tag="KQD82_000375"
     CDS             36207..37367
                     /locus_tag="KQD82_000375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378879.1"
                     /translation="MLISEYIKDNRCGKKSKLYFKKLEEIKQEFINRDLEYLLLTTWD
                     EEISCMMKTGRDLDKLKYRDGFKEINNLFDSISEEIRDEYKEKDTYTMFFSSCSKEKY
                     YIACELANSAYKHIILPNIFEDDKSVNHKVVFKKEQKKEIALMLKLISSYMQHGFSGY
                     KIYDTDIKFIENLCLFEGKKVCYPKPNNLTHFLGDISRSDNDPIEILHARNVINKIME
                     KGYFDNEFELVYFLRGKIMTPMQNKYEKIDTSSLEKINYLEEKALIQKKMILNDEMAY
                     KWKSEINMFKVICKKFKDATHQYRPEWLNGQTLDVFVPSINLAFEYQGQQHFKSVDFF
                     GGEETLEKNKLRDKKKKRLCDENNVKLIYWNYDEPITTIVLKKKLKENNVEL"
     gene            37427..38137
                     /locus_tag="KQD82_000376"
     CDS             37427..38137
                     /locus_tag="KQD82_000376"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG4378880.1"
                     /translation="MEEYLGHFILIGLGVGYIIVYCFIRNVMDNAKEKEARLERIAER
                     NREKTKVEKAIQKNISMAKCQAFGNSNSDSVILSNNGKISIIHAGFKTAVKKNIEDLV
                     SIKFSMQVSEKNQMRIISIVPTYDKYTFVEKIYLTLIFELNTYEVFYIPQYKNINNAD
                     IQEKIKEMERFKLVVENEAAKFKNAKIESNNDIKKDDDDISKISNSLKELQSLKDQEI
                     LTEEEFNEKKKVLLEKIK"
     gene            38196..40079
                     /locus_tag="KQD82_000377"
     CDS             38196..40079
                     /locus_tag="KQD82_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG4378881.1"
                     /translation="MAEEFKIKTSIELDDKKARKQLASLKTSAKENNIKLNVEMNASS
                     LNNLKQLENTLKQINKLSRETQNGLFGGKGNSNGNINKLTSQYDNFRKKIESTQRQLK
                     KFTQTNILDNKQIGEINKLSGELKRLSNIKLGGLNSKALSDLSNVQSKLANMKIPDMN
                     TKMASQFKTLQNEAGKLANKIETLGKSGYADTSKLQALSNSLKSIQNLNLKNLTSNQI
                     QSEIQKLEQLKNKVKEVENATKNTKLDAKFNMNLSKVISDLNRLRQKCLELGQSTAGI
                     DKLEKELMQLNGMPLGQKVKELDSIKSRLSNMKSNFTGLGTSVKATNGFFSDLYNSMR
                     TYTLGNMIGMSITNGVRNIKTTIVELDSALRDMMKVAPDNFEGTSEQLKNVKNEAISI
                     GKDTARASEDIIQGASKALQTGVKSVSDSLKIAKQSAVFANVGDLDQETADKYLTSVM
                     SAYGGMTKALKPIKDTRVQIKGMSKDYDNLTKFLDLSNYAGNNFAITTGDVGAALQRS
                     ASMLSSAGVSMEDSVALIVGGNESVQNAEKVGTALKTIGINMSGIKASADSGKISLNK
                     TAKTLQETAKINVLDKQTGQVRDMMSILDELAEKWHSVGEDALTKNQKSGIAEAINFS
                     RLI"
     gene            40389..46592
                     /locus_tag="KQD82_000378"
     CDS             40389..46592
                     /locus_tag="KQD82_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378882.1"
                     /translation="MDNWNQVKKFQSAWLGGEVLGSASKENERFINSAEGKIIKLKES
                     LKQLVTDTISTDMFKTSLDGLSGITGILNGITKAADKMHVSLPVALAGLSSLFMTIKA
                     LGTGKPITNLGGSIFSAFQNNRKSVKFKDFERATKFTNQYTSAINKNTNASKNNAKSN
                     QVVNRTINSQNGIVGKYTSTTNSLKKATAKEIKINEDRTKSYEKYNQKLKVVSKSRKD
                     TISDGLGNIGKAFAGSSIVNFGKGIATTVGNSLVLTAAFAGVSLLAQAMENYANREEN
                     AYQARKKNIQASKQQINSYESQKVQLQALAEEYDNLSKKENKSKEDNNRLNELKQQIA
                     KIKPDAVIGTDENGIPILKGQVTDLIAEIDRAINAKERLMSYDKHDNAKTAAKKLNSP
                     SKDVNKTLEERMRESETGKLVKIEEDYTAEVEKNKKRQEKAIEKYNNSTGRARDKARN
                     ELMSAKAEEEKIYFKYDEMYRNQVDKIQGYSKEIGDGIFSNIKNKTLYSGLEGNDKSS
                     FAGLESLFDFSEVTPDTLVDTEQAVNKLLTAVRSGKVDVGDLSKTLKDANEEFARTQD
                     IEKYNQTIDKTAKSIAKATNTDANIWENLFGQVNPNGIKDMTSINTLLAKFGKTKLDL
                     ANGDKLAMQLQNQFDSIQNILDTTTITGDVKVDANILTDIKNTKEVPSQVKGMIDALL
                     GTGASSTDVLKFTMDVLMELQTGDPDITKLQNDLDNKFGKGKFTITPEILLSNDSGQA
                     NAEQIISSLKQRYEELPEEVITVIKANPTATLEEADSVKRIYDKFPKEVKTIIKEEGA
                     DEGGRKILDLTSKYAEVPSELKTKLEADGVGLEKAVEVSEIYKKVPAELKTYFIAEAG
                     EALYNSVNLKDALEHIPDEKITEIYLKQNDGKLGVQDLITSLDKIPLDKDVRINIYKA
                     LSDGDIDALGKAIESLPPDKRVEIIAEIEKAKDDIETISSSEIANKIFTIEVKDLASG
                     AIEWIQKKLKEINGDKDKKDPVKEAKESTKKVLKDKPNPKSVAFSRDKEAEKLVDDIL
                     NTPPTKQIELKLKKNEELRNTLNSFKQLEGRNDVKLKLESSGIETEQVKIFADIVKNL
                     PTNSTFTNKFITDNVDSLKNLKDYEAVQKWLMDNPTIAMDYSINLNGLDDFDKMKSIY
                     DGLEDKKDKKVFAEVIIKNPEKAEAFQKLYDNVPEETKSKVVSFAVENADELERATKL
                     YESTPDEQKNQVLNFMLNNEDKLYLIEQLYNDFPESKDIIANLIVNNPDALDEVENLN
                     ALDLDKDVKINIIKSLTNGDINSLIAEIEKLPPEKQVEVIAAIEGAIEGIDSVDKKTI
                     KDKWAKLKANNSAALETIRQTENKKLSDKSFTITAHLRTVGTLGGIASQFANNILGGL
                     KSNRKSVQSINSIETTQAIPANLSAQPRTSEPVPISDETPVTKPSLFSRAVSRATSPV
                     KTLTEKFSRITKTPKIALDTKSIDAAVKYSIELLKELENAISKVTDRISLLDKKMKYA
                     SGKDKLKYLEEQNALYKEELTLLDQKDKALNTQKNRLKDRLKKDYKYTFSDDDNLTSY
                     EEKLISLEKELEKLDKQSESDKGSKKSEERKKKIEEEKKVVEEYLKIAFTEIPKVADA
                     QQEVTNSLIESTRAAEEFKKELKEIAREAALTSAQKHVTEIQNEIDLIDILMKNAEGD
                     EKLDLIEKKKELLAKQAKEIKDVIKVYEDTAKELREGLSKEGFEFSADGKNITNYEQQ
                     LAFLKNNKDADKYKEIEENAKKYLDLLLKDLPDANNQIKQNKEEIENLNKEIQDAYKE
                     QLKEAQSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQDAYNDSRKEAKYKDDY
                     EEQQDVISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKLQDLVQDHVDDQVNDM
                     YDKESDRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVGLDGEVKKLQDAIIEY
                     INKYEDGMLAMGSVTKQEWLDRLKEGKETLEDINDILDELDLSKFAMPNYNPPSNSRS
                     RSTSPISAVNYNSPFVVVQGNVTKDVMPEFEKKMRKMIEENNKKIISNTRS"
     gene            complement(47361..47522)
                     /locus_tag="KQD82_000379"
     CDS             complement(47361..47522)
                     /locus_tag="KQD82_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378883.1"
                     /translation="MEYLVLFLIMIIMITRIINHLTKLIDAILNLKASIRKLKNHQTG
                     GSSDSESNT"
     gene            47784..47939
                     /locus_tag="KQD82_000380"
     CDS             47784..47939
                     /locus_tag="KQD82_000380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378884.1"
                     /translation="MINNIEDCKLLIETEIRMKGIDEKELISQSEILKDLNINEKDLE
                     DIEVNFE"
     gene            48281..49996
                     /locus_tag="KQD82_000381"
     CDS             48281..49996
                     /locus_tag="KQD82_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378885.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNSIKRELGGV
                     DEIQLTIPKYITDRFLFNKIINPIFEEVKEERLICLNNKEYFVVKNVVTTDDKLKVVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEEASIISLNDYLKQETGWKLGYVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMNVIGATVTGYDYIENYSYFLDNKEMS
                     EELSRAIKKYQEMNEIREPIWRELIDTKLKKQRERDSKSNEWQMVIEMISKKKDIKKT
                     YDNPQHKDEVNSAKIAVEISELEDKKVILDVQIKHLEEEIAKLNESIKDINILCKRET
                     STDEDGYLIFNEVLLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTREWTL
                     DVINFLDRIIDINFRQHWKGDLSLGDIIVLHSKESKEEELVYFTSFTQNLKNGKLDTL
                     ELTLSNKKIKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            49998..51920
                     /locus_tag="KQD82_000382"
     CDS             49998..51920
                     /locus_tag="KQD82_000382"
                     /inference="COORDINATES: protein motif:HMM:NF025253.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBG4378886.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDKETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPINSSIFNISFDGNSRKNTEEQIWGLY
                     ETDEEHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRAENVNILVKEVTKN
                     FGGSQENIILRENINKAIIKLNTDLGTFSSNMSNYFNDNEITDEEKEKIDIELNLLDT
                     DKTSLYIELQKLIDRTTGVDLVAINTSKTALNAASTNLNSIINSVISDGIITPSDRIL
                     AINANAQYNLKINELKNTVDKIYITGMGGSISEEFSQINATAKEIKLEVAKVDGATKT
                     NAAEIKLTKDDITMMVTRNNSGSIVGIKPDKIEFGFNDISNYVEISRSGLTVNQGAIA
                     CDILTTPSGHEPIIRLFGSSRSGFAIDARQSDGSSQASAIRLKYDSNNYFWVGYDTAE
                     IYVDGEEHFIVERDDTFVRCGGATFTFTNGDSLGVGYSFYPERSVTDLGCERYKWRYL
                     YARSTLSESDKKFKENIVYIKDIKNRTRSSITPTPFLDFIRDDFKPATFDYIAEKDRT
                     IADSQIGFIANDFKDSYVGKTFLYDYGEENGLMFSPSGYTTVVATALQEEIQKREELE
                     MIINELNEKINNLGGY"
     gene            51922..52077
                     /locus_tag="KQD82_000383"
     CDS             51922..52077
                     /locus_tag="KQD82_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378887.1"
                     /translation="MEVNLQKAYTVAFEEIKSLYNELILYKALNMQQQEEIENLKKEL
                     EEQNKEQ"
     gene            52089..54167
                     /locus_tag="KQD82_000384"
     CDS             52089..54167
                     /locus_tag="KQD82_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG4378888.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGIVEIELSRQALASVGINTF
                     QLSLVKDGTLLNTTNLYYRVEEGMINDDDITSTDEYGVLLVIIAQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQEANREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSKEIDNIVDDKMLDVQVQTDNKFQDLDTRANNTFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDESTTKLDTKIKEVDDKIVEVNTA
                     KTDMTTTVSNKITEFENRFEELESLDARGELIQARESVDGTVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVADGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTTVDSNTIEIFNESNIDVKVTVVNGNTNKEVIQATINDNITTLDSTYSSIKIDAK
                     FDESLTKINENKSNIATNLEKINLIQSKVGSSELSTVSKNISDAVNELDASVKVLESG
                     GNTNEQLKILKENYNKMSVEILKILFELELDKEATADEAGYWYDTLYDSKNISNLDGL
                     KLNKFKQQLELLSNSGTVLFKNVTVPFLCNKIRYIHDLGSNYSETKTEESYQKGDTEI
                     KINKYSYEVR"
     gene            54178..59457
                     /locus_tag="KQD82_000385"
     CDS             54178..59457
                     /locus_tag="KQD82_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378889.1"
                     /translation="MKKNKLLQRGSLFGNKEFKIEEFNKNFDTFNVKDIFTDMDSGSV
                     FIIKDDLKLYAAGYNVFGNLGLGNKVSPIRYFSKIDIDNVKYVSSSGTHSLLLKNNGD
                     VYSTGLNTDGELGLGDNIDRNTFTKINISNVKQIACGNGYSMLVTNDNELYVCGNNKF
                     GGIGLGNTSTVNTFTKIDNLSVKEIFAGVSCSYILTLNNEVYSTGTNSYGQLGLNDTT
                     NRNIFTKVNLDNVIKISTAQGAVNFLLENGDVYGCGDNSNGQVGVGGGATVTVLTKLS
                     ISNVKNIESGRYHKFYIANNNDIYVTGANPDGRLGDKINCDRSTYTPRKSSISIKNIS
                     KIKCAHAISFILTSNNELYMTGDNSYSFSYLKRYDKFIYGFEKFEDFDIEECNHIESI
                     DNRLLINKNDERVLDIGLENNFVNISLVKANDTSTFIVVNGNELYAAGYNAYGGLGLG
                     HNRHCYEFTRVPLELEEGVTIKDIYLNLGSYFTFILLSDNTLYSTGYNVFGVLGLGDN
                     INRNTFTKVNISSVDKISVGDNHALLKTTTNELFSTGLNNYGQLGLNDTTNRNIFTKV
                     STVTYVNEIIAYSNSSYCIDNSRISYAAGYNVFGELSLNDTSSRSVFVKMRYSRYKTD
                     TTVSDVSNIAEIIPFNNGCLIRTTSNNYYVSGRHNYYSTSIKNSVTDDYYSRFGEFVG
                     SKNSNANEVAVLIDEPKLSKVLVSNYNLCLLLDASDGGYKEIKFTGRPSTFGASSQDK
                     NLGLRSLISSSMPYYNHYLGTDNLLYTTSKTRMESKFEIRGRNAGGQRGDSSYYSNSQ
                     KYTLSNAFFAYNSQVIYASGLRIVIKDYFGNIYCSGVNSHGCFGIKKPYDVRHDGFVN
                     ISKQFSKKYIISNIKEIKSDRQALYILLNNSEGYVCGNNAGNKLGTIKARSEFIKVPI
                     DNIKEFIPSNDCLFILTNNNQVYSIGTNDLGQLGLGDNIDRGTFTKVPIDNVKKVITC
                     YKSSYILTYDNELFSTGSNGYGQLGLGDNINRNTFTKVNISGTIKDIFCGRYVFIILL
                     ENDNIISLMGCGNRNYISSNTNSNSLTKINIEGLTDPSTISNLMLEEGKTIITMKNRI
                     FVTGYNSLGGLGVGTSYNNSSGYIEGYKDLAFNSNIKEVALGEYTIFVLLENGDLYSG
                     GNADDGIGFNESGTVVLKKLTSNIKHIYANGKNAYYIDNDNALYVFGSRQLGQINNYS
                     DVVQKTPVRNCLNVKQVYTQHNGLIVQYTNNKLEGKGNAVVGQVGIFNGHEENQIANT
                     SGDNPSDLSFSNEMSIIRNNYGLLVNKKIFLHNDDSINYRIPFGTNAGNYSTSLPFDN
                     IKKMSLSSTHSILILENGDVYGCGSNQFGELAINKSELTTTSEFIKINLTNIIDIACG
                     DNFTYFLKNDGSLFSIGKNSEYQLGIGHNDEVNELQEVLTISNIREIYAYSNYILAIT
                     TEGQLYVQGYNINGILGLNENTKNTIIKLFTKVLENVKYIKSYDDKHIFVIKNDKTLY
                     VTGINKETYKIKDVEIGSMLYTFSQIYIPRDVNDIVDALIKDETLYIISKVDSEKTCL
                     EIKNKSYSSIEIDLQNPNNELTKIEMFINNISSSIIEDLSTGTVTFEINPENLVLGEN
                     KIVFKAHSDIGNNLYINVYIYKKETGATIVKDSTVLIDGNTYNVSSIVDNSQDVVLTL
                     NKGLLEDLNSNNPIYHLVNKLKVQLKINESDTFKDMVKVETRKTENGYKEIYELKDMN
                     IQSAQPKVIVEEGNTNTTIKKPSMLFNLDVETL"
     gene            59469..59792
                     /locus_tag="KQD82_000386"
     CDS             59469..59792
                     /locus_tag="KQD82_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378890.1"
                     /translation="MIKIDKEKIEKSRQRKELDDLDSSQKTQDNDITDLMLATAEICE
                     MVLSSQQTSTMSLKNIKLNEGGSSMAAIYVGLIERGLKTIDQVPVKYREEVRKMCEAL
                     EISLS"
     gene            59872..>60192
                     /locus_tag="KQD82_000387"
     CDS             59872..>60192
                     /locus_tag="KQD82_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378891.1"
                     /translation="MLNEDVVKKLKNVPNNTNTEIEKVNTNIETAKTELNTKIDQLIA
                     GGSNVASTQTITIDDWVDDTENGFKATVTHGLLTQRIVVNIIDATTKENVVTNFKIID
                     DNSIE"
BASE COUNT        24736 a         6442 c         9696 g        19318 t
ORIGIN      
        1 tttattttta ttatagattt ataatcttct tctgaattta tttttattac atcaaagaca
       61 atatttatat caacatcata atctttacct aatcctgaat atgtataact tccacaatct
      121 aaataattat atacttcacc ttccataaaa aaatcccatg agtcagtttt attaacaaga
      181 taattatatt tttcttgtac aaaattatca tattctaata ctgtaaactc ttcacctaca
      241 gaacttttta gttcttttat aaagctcctt atacatcttt cattttcttc tatttcctca
      301 ttgtaatcta taatcatatt atttacattt tcatgcacta ttgaatctac atcttttaaa
      361 ttatattctt ttcttttata gtcttctata atatcatcaa tgtaaattat ttcttcgtaa
      421 gtattgttgt atatattttc gtctgcaccc tgtcctttat atgaaataaa aaactttatt
      481 gaatcatttt ttatataaaa tccgcatcta tctatttccc aattaaaatt attacttata
      541 gcttctctta catatttttt gatttctttc ttagttattt ttttcatttt catatccccc
      601 tatttttatt ttttcaattt taaaacctct taatttacca tcttttataa taaaacaaaa
      661 tccctctttt gctaacatat cagcatcttc taaacttata ttatttatat ctttcatata
      721 tcgtatcata ttgcaacctc aacatcaaag taagtgccaa cacaattact taaatcaatt
      781 ttactatcta atgttaaata ccaatcaccc atacaagaag gataaaaata atatttatta
      841 tcatttttta tatatacaat tgaacttcca tcacttaaaa atactttaat gtttccattg
      901 ttatttaact tattaatcac atatacatcc tttgagtgac ttatattgtt tgacagctct
      961 ttgtaagttg caactgctct atctaaatct tttttatttt taaagctata gttccagtca
     1021 cctagacaaa ctggatagaa attataaact tgctctttag tatcatataa aactacactt
     1081 tcatcttcta atatttctac aactctgtcg gtagtttcta cataaaaatt atctaattcg
     1141 gcagccttaa cttttgttgc tcctgcaact ccaacaataa ttaaacctaa taacattccc
     1201 atagctatat ttttgaattt catttttaca ccctcctagt ttatgattat gaccccgtac
     1261 aagcctctat aagctgtttg aaaattatct taatgtattt ataccttttg atttttataa
     1321 tctttaaaat ccttttatca aatcactatc attatcattt gaattataat aatatagcta
     1381 taataatagc gatttgataa ctcatatatc ctattaaaaa tataatttta aactctttta
     1441 ttacttcata ttctatatta aagatatgac actcttttat ataaaatata atcatcatct
     1501 atataaaatt cattgttatt aatttcatcg cctacataca tttattgcat acttttatat
     1561 agttctttta aatcttcatt ttcatctaat attactttta aaggtttcat agtataacaa
     1621 cttttatatt tatatttgtt tttattgtaa ttttcatcaa atttactcat atattcctta
     1681 tacttgataa acaaactgtt taatatatcc atatctatca atccagactt actaagttta
     1741 ttcattctca tacaactatc tatcatacga gccaaagacc aagccacttc cgcaattgca
     1801 tcttctaaca tttgttcatt agatttataa gcataagaac cttctttgta tttagtatta
     1861 ttcttcacta ttttattaag atttgtctct atatctaaac ccttactgta gtactccata
     1921 catttgtaat gtttttttat ctctctcgac aatgctttgt aaaatgtaac attcaatttc
     1981 attatttttt ctacacatgt taatccaaca tgaaaaactt ctcctgtaac attatttttt
     2041 attgtagcta tattttttat agttcttcca cagtggtcac aagctccttt accttttgta
     2101 taacttatca aaatatactc attcatgctt aaacctccaa ttatattaaa attaatcaat
     2161 taattcatat attccataac cattattttt tgtcttataa acttcttcta gtatccaaga
     2221 ttgtaccatt gtcatatctc taccttttat tttattggta tatgctatta aaacatctat
     2281 atttttttta tcctttttta cttctattaa aactttctta gaatcactat tttgccatgc
     2341 tgatattatc atagtaatca aacctccata ttatattaaa attaatattt taagtacttg
     2401 tattttcatc tgtaataact ctttatatag tttaatagtt tttcttctac atctatatca
     2461 aagacagttt tacaccctac tatatttttt aaatcattta aagtaagcat tattaaatgt
     2521 gtattattca aatctaatcc atttacagtg tttaaattat atgtatctaa ataatgagat
     2581 aaaccacaac caaaactata atttgctaat ccatatttat atctaaatgg tgctatatgc
     2641 tctactcctt tacacaactt tagtgtgaca tcctcaatgt tattattatc tattaaaaaa
     2701 aatctagttt ttattgcttt catacttctt aagcaattac ctatgcaacc aattaaatac
     2761 ttattcattt taaaccctcc atttatatta aaagttgaat tttatttata tctcttttaa
     2821 tattttttga taattactat gaagtaatat tgttctacat ttttcattat atgttcttat
     2881 ataaacatcg ccatctattg ttaaagtatc ataatcttcg tatccaattt gattttgtct
     2941 agttagtaaa ttgtatattt cttttctttt acaccatcct gttcctatat aaaataattc
     3001 tctctctgtt ttagaattac tttctctttt actttttttg atatctaact ttttaattaa
     3061 tatatcatag tctatatcaa gattaagttt taatttataa tgtcctgttt ttgatatagt
     3121 aaaaattatc caactagagc tatcatatat tttcccatca tttaaattaa tattatttat
     3181 atttttttta ataaaactat caaccaattt aaatattcta ttttgtctag tatatttttt
     3241 tacatttctt acaagtattt ggaaaactgg atacccttct atatcataaa atacgattat
     3301 tttattcatt ttattttcct cctataaatt aattattttt tatttaaaat tctttatatt
     3361 gaattttatt ttcattatct aaatacatca tattattaat attttgtatt aattgctgtc
     3421 tcaattcttc tccttttaaa gcccaatcta atccgattaa gtttcctagt ctagtactta
     3481 aggaattata agttcctgct tctcctattg tttcaccttc taaaatgtca catatattta
     3541 ttaaatctgt tgcagtcata tatttagaat aatttatcat ttttaaccct cctaatattt
     3601 tacatctatc ttaagaattg aattttataa aacaatagct ttagcttctg ttatttcatt
     3661 attaaatttt atattttcca accatacacc acttggtagt ttttcatcaa gtgaccacag
     3721 gtaacaatca acatcattat gatatattgt tgggttgaaa ttttctataa aaagtatatc
     3781 ctgcttattt aactcttttg tttttatgtc ccatactaca tttgatattt tgctatttat
     3841 tttcattttt aataatcccc ctttataatt tattagattg tttcaataat tttaattgat
     3901 tgctgtttcc acattttaaa ataatacttt ctgtagtact ccaatagcta ctatctaaat
     3961 cctttttaaa caatgatttt atcttaatta ttaatttttt catgatttat tcccctttca
     4021 atttttttat tggaagtggc gtttttaaag aacgccctaa aactttttat ttaagaaact
     4081 atagctaaat aatttgatgc tatacaaata ttctcataac gtaagtcagt tccatatagc
     4141 tcataattaa agtatctttc aagtgtttct tgacctaact cttcaatatc tcctaatcgt
     4201 tcaatataat tctctgcaaa ttcaatatct gtcatatttt cataatattc tatatcttct
     4261 tcgtataaat tttcaataat catatctcta tcaaaacgta aatctcttga aaatgcttct
     4321 atgtcaaagt agtaagcaat attttctata caagataaat caccacaaca tatttcttct
     4381 atgtaactac gtcctaaatt ttcatcatcc aatggtgacc tgtcatcaag ttgtataaaa
     4441 gtataattac ctgataatct gcaaatatcc ccttcaagca tgtcataaaa atcaatgtat
     4501 ccacgttcaa gtaaagcttt taactcttca atctcatatt cttctaacct ctcaagtttc
     4561 attgctaatt cattcagttg ctctatattg ctatgttcac ttatttctaa cccttctata
     4621 tctgtttcaa aatctgttat gaaatactcc tcatattctt catttatccc tattctatct
     4681 aatatttctt gtaattcttc ctcgtttact ggaagttcag cccattcccc tattagttca
     4741 ccttcgttat atttccctaa attagttatg tatatattta gcattttata tcctcccttt
     4801 ttactgactc gttactagtc attttgtaaa aaattttatt ttatgattta attataatcg
     4861 actcgtcacg agttgtcaag tatttttttg agtagatttt ttatctactc atctattttt
     4921 tctattacta ttttattacc atcaaaagtc aacaaaactt ccctgtctag tttagttatg
     4981 tccatatgtt ttatccatgt agttggaatt gtaaccctat tcgtatatga ttctccccta
     5041 gaattaccag ctcctttatt gaatattact tttgcttttc tctgctcata cattcgttat
     5101 acctccaatg atattattgt ttaataatac catactaaga gcttgtagaa aagacactca
     5161 ataattcaag tgtcttttta tgtactgcta tttaattgat tttgctgttt cattttgcac
     5221 tatatcaagc ttagtataca atgaggtttg accattttct aggtcttcaa ttctaaaatc
     5281 aatacgacta aaacattcta aaagtccctc aacattagat tttattttat cttgtgtata
     5341 atcaaattcg tcaagcttgt catttaaaat tttaatactt tcctgtattt ctcgtagcaa
     5401 ttctaatgtt ttctcatcca ttttatctac tcctatttaa ttgattttaa ctttgctatt
     5461 tcattccaat ttttagatgt agccatttct acaaagttca tgtcattttt taaagtgtct
     5521 attttttctc caacttctgt tatgccttcc atatttctag caacttgatt ataagttaag
     5581 tctagtttct ttttaatttc ttcttgtcct tcttttaaat cactaagttg gttttcaatc
     5641 ccatcaaaac gaccatccat actatctagt ctagtattaa tccattttat ttctccttgt
     5701 atatcattaa tactagtatc cattttttgt aataattcta atattttttc atccatgccg
     5761 ttcacactcc tattttatta ttatatcata atttaagtgt tgtattctaa cacttactaa
     5821 aataatctaa taaatagttt aaattatact agtaaatatt agatagttta taaccatgta
     5881 ggtgtcaagc agtacaaact ataattttaa tgctcaatct ttagtacttt ttataacgca
     5941 gtacctcaca actgctatgt catattaaat tgtattgagt gccattatag aagcaactaa
     6001 gttgatacct caattttttg taggctttaa ttggaaacct ttattaattt tatttactta
     6061 attacttaat gttgggaaca cctcaccaac tggtatgact aattcatttt tgtagttaaa
     6121 aaatgaagaa aaaactatgt gtaatatttg tgttgtgtgc ctttcaccat tcacaccatt
     6181 aaaaggtcaa cttaaaggta gtaatttttt aatagtagtt taatattttt taatagaacc
     6241 ctttaagata ttagctagag ttttaagaag gttctttgcc tgttacttca tttgagaggt
     6301 ggctttgtgt tttcttgttc tcccttgcta tgatttaatt atatatccaa acggatatat
     6361 tgtcaatatt tatttttata atttaataaa aaatatatct aaacggatgt taatatattt
     6421 agatatacga ccgattggat atataactga ttgttcttaa taaatatata tgatattata
     6481 ataaatataa agaggtgata aaatgattag tgatagatta agagaactaa gaaaagaaaa
     6541 aggctacaca attaaagagt tagcacaaat tataaatgtt tcagatattt caatcagtag
     6601 atatgaaaac aacaaaaggg atgcagatat aaaaacattg aaagagttag caaaattata
     6661 tgatgtatca ttagactata taacttgtct tacagataat agagaaaata tacaacttga
     6721 agaagaagaa gtcaaactat tagataatta tagagagtta gacactaaaa gtaaaacaat
     6781 agtacaagaa caaataaata cattaaaaaa actactgtaa gctagagcat atatgtctag
     6841 cttattttta tatttaattt taaagattaa aggtataaac ataaggtgaa tgcattgaaa
     6901 tgcgcttaaa atggatttaa atggcttgta caatatgtta ttataataaa atagactaga
     6961 ttatagctag tctattttat attattaaat tattttattt aatgttaagt actaccttga
     7021 gtagattata ttactatatg tcaagtagtt cttttgattc tatatctaaa tagctacata
     7081 ttttttctag ttcacttact gtaaattgat tctctccttt ttcttttctg tgataatttc
     7141 ttaaagatat gtctaacatt ctagcaatat cctcttgaga atattttttt tcttttctta
     7201 tatcaattaa tttttctaat ttcatgctat cacctcatta atattataac aaaaaagagt
     7261 tacttataca ttaagcaact caataaagat attaaagata aacaatatat attgctctaa
     7321 gtgcaaaata taagaagaat aataaaccta gtatctttat tatttttaaa gtgtttttaa
     7381 tatgttgact catgtcgtca cctcgtttac tttcaatatt aggtgtgtta caatatacct
     7441 atagagatat atttttatag gtatattgca ttggttatta atgtttaatt atgtatataa
     7501 gtattaataa ccaaccgata caagaacata aagaaatcaa aatatgttca agtgatttaa
     7561 atacttgtac aaagagcttt tcaagttggt ggcacaactc gattagctct tttattttgc
     7621 cttttgttat tctcataacg tcacccccta atattgaatt gtatagataa gattaattct
     7681 tatcttaatt aaattatata caataatgac aaatttgtca agaaaaatat ggcaatgagg
     7741 ttgtgagttg atattcgacg atgtcagatt aatgctaaaa tgatgtatat aagactttat
     7801 aatccataac aaaaaaattt attctatagc aaataatatt ttatatagat atcaataact
     7861 aaaaattgaa atgcagtgat tacaacaggt ttgtagtcta agaattataa tgactgacat
     7921 tttactgaca aaatagaagg tgtaggtgtt gtaagtgttt aaaatactag gttttagaaa
     7981 aatggcataa aatacttaat tttatgccat tttttgctga caaaaacata gatttttaaa
     8041 ataagataaa agtaatactt ttattaatta aataaagtaa atagagtgtt ttacatatta
     8101 aaataaataa aaatgaagta gggggtattt tatggaaagt ggcttgtttg tcagtaggat
     8161 tttgtgtgta gtcctttcat ttccacattt caaaaaaatt gcagacggat ttacagacga
     8221 aatagctctg tattttcctc ataaaacaaa tatttatcta aagatatttc ttagattttt
     8281 actcttaaat tacattctta acaaggaaaa tataaaataa aagtgtatgt attgattaca
     8341 taaaaacaac taatatatat aaaaatatta aatacaaaaa ataacttgaa atataggaag
     8401 atttattaaa ttagaatgaa aatgtcgtct gtaagaaaat aagtttcaat attttactta
     8461 aaattaaaat tgacatctaa aaaaacataa atagtatagt taaagaaatt gatttattgg
     8521 tttggaaata gttgatatat aatttaaata ttaactaaaa atttttaata ataaattatt
     8581 ggtttaaatt taaattatat atagttctaa tttagtgcta attttaacta ataactaaaa
     8641 aatgtcattt tatagttgaa tttttgtgca aatttaatgt aattatcaag tttttatatg
     8701 gtataatata tttagcaaat atttacttag gaggaatgaa aatggaagaa aattaccaag
     8761 ttataggctt agatatagga agaggatacg taaaaggata tagtaagtac aatggaatgg
     8821 ttaaagaatg tttattcaaa tcagtatttg gtgatggaag aaacatagat tttgagaaat
     8881 atgaaaatcc aatatacata gattttgaaa gtgtaagtta ctttgttggt tcattagcag
     8941 aaaaagaaag tataacacct ataagaaatt ctgatgattc taaagtatct tttacaatga
     9001 gaatattagt tgctgctgct cttaatgaaa tagctgtagc tgatgaagta aaattgatga
     9061 tgggcgttcc atataaatca tttaggaaaa caacattaaa agaagttgta gacacatata
     9121 aaggtaaaac atttaaagta aaagataaaa taaaaggtgg acataaagaa ataaaaatat
     9181 ctgatatatc aatttttaga gaaggagatg ctgcattatt tcatacatta gaaggaaaag
     9241 taaatgaaga taaagcggtt ggcatggttt caataggttt tagaagtact gagatgtctt
     9301 tctttgaaaa aggttttgta tttaatgata aactatcaga cacattagaa gctggaaatc
     9361 aagatgcttt aacaatggtt cagaagcaac taaaagatag aggaataata agagaactta
     9421 atgagataga tagttctaat gattatgatg aacttaagaa agttgcttac attatggctt
     9481 ctgaaagcac agctcaaaga atatcttcta aatggaagaa catagatgaa atggatgtat
     9541 atgtaagtgg aggtacagca ttacatatga cattcgataa tagatttaaa gtttctaaag
     9601 atgcacaaat ggctacagct aaaggattat ttgaagtagg tatggaacaa ttctaggagt
     9661 gatattatat gaaaaaatca tctagctttt atttagaaga agatatattt ggtgaaatag
     9721 aaaagtatca aaatgataaa aatataagca gtagaaatac agctttggaa agaataatat
     9781 tagagtggaa aaatcttcaa gaagaaaata aattattaaa acaatgttta ggcaatggtg
     9841 tatataggac aaataaaaag acaaatgtta ctgaagaaaa aaaagatata aaaagagaaa
     9901 atcctattat taagaatata tttaataata tgccagatta aaagaaaata tctactcaag
     9961 aaattttggg tagatattaa aaaaaatatt gagaacatag agggtatatg atataataga
    10021 aatagaacat agagggtaat gaaatttctg aagcaacatg ttgcgaagaa caattgcaag
    10081 atgattttta tcttgtaatt gaaataaaat ttataagaaa agaggtagat atttatagtt
    10141 agaggtaatg aatttataaa agatgttatt gtaacagaca aaagaacagg agaaatattg
    10201 gagcataaag ggatgaaaag aggtgaggta cttgaaattg gaaagagaaa aaagagtttt
    10261 tctccagagc aaagggctat tatcaataat aaaaaagaat taccatacca taattttaaa
    10321 ttaggaggtt ttgtattttg tacttatgtt aaaaatgaat tattatttaa tgaagtaaat
    10381 ataagtaaag ctaatataag tagattaata tatctcgcaa catatctaaa ctataataca
    10441 ggagaagaaa atttattggt tagatattct cagtttaaag aaatgattcc tatagataga
    10501 aaaaacatga gagaaatatt aaatcttaaa gatagggcat ttagaaattt tttagcagac
    10561 gtaaaaaaat gtgagttatt atatgaatct aataataaat tttatataaa tccaaagtat
    10621 tttactaaag gaattcctaa ttttgaaaat aaagaatata caagaatata tataaatact
    10681 agtagatttc tttttgaaca ttgcacacca agacaacata aacaattaag ttatatatat
    10741 caactcatac catatataca tttcgagagt aatatcttat gtttaaaccc tcatgaaaca
    10801 gatatttcaa aaataaaaaa aattagttta tataaaattt gtcaattatt aaatattagt
    10861 acaataaaac aagatatgaa caaatttaaa aaggaattac ttagatttta tataaaggta
    10921 gaagatgata aatattattt ttttaaatat gtaatcgttg aagggaataa caaaaataca
    10981 gactatttca ttataaatcc ttgtgtaata tggaagggaa ataatttaaa tttagcaaag
    11041 aaaacaattg atttatgctt ttttaattgt atttagaaag tctgtctaag aataagaggt
    11101 aatgaactgt aaaaattaac aaaaatatag gaaaaacagt taaaaaatta aatttagggg
    11161 tggcacagaa atgtgaccaa aaggcaaaaa aacggcacag aaatgtgacc aagaaaatta
    11221 atttaaaatc ttataatata gaaaaggaga agtatgaaag agaaaaaaat agaattaata
    11281 tatttagaag atttagatga atcagagaaa acaggcgtac ataaaaatag gtatcaaata
    11341 ttaaatgagg aatatgaaca aatgatagat acatacacag agatgagtaa aaggtcttca
    11401 tatgatattg taaatgaaag gcataaggag gattttaagg atgaactatg ggattatgat
    11461 gatacagatt atgatgatgg attgcaagga aaggaggtat aatattgata tataataata
    11521 acaattttta tgaagaaata gatggcaaat ctgcacaaga ttacataaag aatttagatt
    11581 atagcataga caatagcaaa gatagaatta aatacataga ggaacgatta ggggtaaaac
    11641 acacgtggtt tgaagagtca aatagaaatt atatagatac attttataat actcttacaa
    11701 agagaaatga gaatattatt actagagtga aatatcaaga aaaaaaacaa ttcaatgata
    11761 aattttggga aaatatattt gagcaaacat cagatagtcc tttagataaa gatggtgtat
    11821 attatgttac tattgatgga gaagaagtag ttatggatta taatagattc attacttggt
    11881 gtggctataa tcaaattaat ccaatcgaat atataaatgt taaaaatcct tttagtaata
    11941 gtaatggtac atgggagtac acacatcata atacatcgaa aattaagttg atattaaata
    12001 aagatgacag tatatattct acttctaata tagcaaaaac attagaaata atggggagct
    12061 atatactctc agtagatgat aaacctaagg aaactaaaat aagaatatac aattcaaaag
    12121 agttatttga tagagcatgt caggaagaag cacttctaaa taaaatagca cctgcaaatg
    12181 gtggaaatat aaattcaaac acttctaata aagatggatt cctagaagac aattcctttg
    12241 cattttttca attacctaaa aactataaaa aaataaaaga tataaaagtt aaacctaaag
    12301 atattaaaaa atatccaatt ataaaaacat actcagatac ttatgaatgg tataaagcta
    12361 aatataaaga attaggacat aaacaattaa gtaaagaaga gttaaaactt aagagaatgg
    12421 ttaggaaaaa tttaaatgca ttaaaaaatg atatgactga tgttaaaaat tctatagaga
    12481 gaccaattat atggaaagca ccattagcag atgcaggaag cccagaatgg gactatttag
    12541 atatgtttga taaatcacat gttaaagagc ttttaaagat tcaaaagggt aatgatttgc
    12601 aagatgattt aacatgtata gttatggatt taaataatat tatatctaaa attgagttta
    12661 ccacagtaca aaaagatatt ttaaatttat atagaaaaga taaatcatta gagtttattt
    12721 caaatttaat gaatataact cctcaagcaa taaataatca aataaataaa atagtgaaca
    12781 aaataataga tgcatatgag aaaaactatg aagaaaatta ttattatata tctgtttgta
    12841 aaggaaaata taaaaaatgt agcagatgtg gaaaaattaa gttgctatct aggtttgata
    12901 aaaatggtaa aaagggatat cgctcaaatt gtaaaaactg taattagctg gtggggaaaa
    12961 gtggtttaaa aaagggtcgc ttaaaacata ttacttatgt aaggggttaa taaaaataat
    13021 aaaattattc cttactatta tcaaatgaaa attatataag aaaagggaga ttaagaagat
    13081 atgaaaaaag tagatttttt aaaagaagta gcagaagaat taggtgtatc acagataaga
    13141 gtaagagaga tacttgatgt gatagaaaaa aaaagaaacg aagtactaca agatggtgaa
    13201 gaattgacgt tgatgggagt taaataccta actaaaatac aaaaaggtaa agaaggagaa
    13261 ataactctta aagatggaac taaaaaacct tggaaaactg aggaaaaaag agttccaaaa
    13321 ttaaaaatag ctaaatcttt aaaagatgca atcatataga gagtgtatgg gttaaaatcc
    13381 ctttcatatg gtgacatatg gcagcaaacc tagaatatgc taaaaattaa tacaattagc
    13441 agagatagct cattttgaga agctctagtg gctggtaata ggtattttac aaatataaga
    13501 tgatgatgct gctaaaatga atatagcgtg gcaaatatac acgaggttga tgagcaacat
    13561 ataacttgaa atagatacgg tgaatgccct agtatgcctt aaaggtgtaa gaataaagaa
    13621 gtagaatttg aggtaacaga ttctacttct gctttttaaa attagaggag tttgttggtg
    13681 tacctggcta ataaaaaact gaagaaattg tgaataaatc aaatagtctt ttgaactaag
    13741 taatttttat gtggatttat ttataaaaaa gtggtgatgt tctaccaaat agtggacaat
    13801 taaaaaagat tctttagtgt gaaaaaacag ccacgctatc cgagctgtcg gagttttaaa
    13861 atacagcact tgaccatggt cgaaggtagc gatactcgac ttgctgtttg taatttatag
    13921 aaatatggag aggtactcaa gtggtaaaga ggatagtttg ctaaactatt aggtcaatta
    13981 atttgatacg agggttcaaa tcccttcctc tcttccaata aaaaaagttt gttattttta
    14041 aataatatct taattaaaag ggagatgttt ttatggttaa atataatgtt cataaagctt
    14101 tagcagaaaa gaagttatta gaagataaga tagatagatg cattagtaat tttaaaattg
    14161 taggtactaa aaaaggctct gataaaaatg tatatgaaac aaaaacaagt gtagaagatt
    14221 ttaatgtaga agtttcaagt agataccaac aaatagaaga tttaatatat aattataacg
    14281 cattagataa agcaattaat atctctaatg ctataacaaa tgttcaaatt ggtaataaaa
    14341 attatacagt tttagaagca ataaaaagaa aaaatagtat agaattagat aaatccttgt
    14401 tgagacaaat ggttagtaac tatgattgta tgatgtctga agtaaatcgt agaaatgaag
    14461 aagtgcaaaa aaatacagac agaatgtttg aggaaaaaga aaaaagtaaa gatggagcag
    14521 aacttatttc attttataaa aaacaacaag aatggtcttt agtagaccct ttaaaagtaa
    14581 gagagaaaat agaaaaatta agggatgaaa tagaagaatt tgaaaaagaa gttgattttg
    14641 cattgagtac atcaaatgca cttacaatta tagatgtaga tttaaaatag tccttaattg
    14701 ggctattttt taatttgcag gttaggcgaa taacataaat tataagttcc cagttgattt
    14761 tgggttaaaa atcaaactac ttatataaaa gtgatgggta catacaaacg aaacgattgt
    14821 actgtataaa agagaacaat agatataaag cttaaagtta aaaattcaaa tataaaatct
    14881 caaggataaa agattaaatt attaaaatag taaaaataaa ataaaaaagt tatagaaaat
    14941 ccttgataaa tgctaaatgc gttaacttat attagctatt gttaactaca aggctgccta
    15001 atttgcaaat aaatatattc ccagtagagc tatagataac ataaaaataa aggagaaaat
    15061 atatgaaaaa taataaagga acgattttag aaaatgtgaa gtaataaaac aagaagatgg
    15121 tacttataaa tttatagagc atacaaaaaa tggcgataat gaagtgttat ctagtgatgt
    15181 ttttgaccag tatattggaa aaacagattt aaatataact ataagattaa gtgaaacaat
    15241 agttaaataa atcaatattg gactatttat tttttactta atacattgat aatgatattt
    15301 atgtgataaa tagggagata tcgaatatgg aagacatatt gaagaaaaaa gaagatgagt
    15361 cactaattga ctataggatt agaatacgac tggcaaaggt taataaagag atagatttgg
    15421 attggggaga gatagttgaa ctattagggt tagaatgttc tccagaccat tgtaggaagg
    15481 tttcttatgg gttgaaagag gcttttgatt atcttaattc taaagtacaa gataattcta
    15541 ctcaagaaga gattgataaa ataaatgaga aaatattaga gctgaaaaag ataaaagtac
    15601 agttatctga tgaaaggtca ttagtaaata agaaaataag agaatattca agaatagata
    15661 atataattga tttatttaat aataaaatag atgatatatc acttcataag ccatttctaa
    15721 gtgattctag ttacaaatgc tatgaatctt caaatcaaga ggctattatg ctaatttcag
    15781 acatacatta tggattagag actattaatg catttaatag atataattca gaaatattca
    15841 agataagaat acaatattta aaagataaaa ttattgaata tagtaagtta cataaagtta
    15901 agagactgca tgtaatgtta cttggagatt taatttctgg atacatacat aattctataa
    15961 gattagaaaa tagagaaaat atagttgaac aaattataga ggtgtctgaa atacttagtg
    16021 aatttatata tgagttatct aaagaaattg ataaaattat tgtttattca ataggtggaa
    16081 accatgatag agttcttcct aaaaaagatg aaaatttaga taaagataat tttacattat
    16141 taatagatga atatataaag ctaagaatta aaaatataaa caatgttata tttcaaaaaa
    16201 atatatatga taatgacata atagtggcta aaatatgtgg aaatacttgt tttggagtac
    16261 atggagacaa agataaaatg tctacagcaa ttcctaaact gacctctctt ataaaattaa
    16321 ttcctgatta tatctttatg gctcatctac acaattgtaa ggaagataat tatggagaaa
    16381 gtgaaatatg tgtaaatgga agtttctccg gaacggattc ttatgctaag aatctaagat
    16441 taagttcaca cgcaatgcaa aaattaatga tatttaatga aagtgggaga atatgtactt
    16501 acaatataaa tttaagtaag ctataagtta tttattaaga gctgagttaa gtttatattg
    16561 taaatttaat tcagctctta attgagttga atctccctat taaccactag agatttaatt
    16621 ctctagtggt cttttttaat ggtggtgatg ttatgaatgg caattagtaa aacaaagtgt
    16681 ttgaattgtg gtgaagaaaa atatacagat agatattttt ggaaaagtca tagtgaaata
    16741 tttactttaa ataaaagact tccagtatgc aaagaatgtt ttagagctag gtttttacta
    16801 ttaaatgggt gttacaatgg agaacttgta aaggcactaa aacatatatg tttcaacttt
    16861 gatgtttatt ttgatgaaaa attagctaaa gaacttgcag ataaaaaaaa taaagatgaa
    16921 ttaatagatg aatatatgaa aattattaat agaaattcaa aatataaggg aaagacatct
    16981 ttagataatt tattgaaaga aatatataca ggaaataata atgataaaaa tatagtcata
    17041 aatgatgaaa ttaaattaaa atggggaaga ggttttgatg attatgagta taaaatctta
    17101 gaaagaaaat ataaggaata tgaagaatat tatgaaccag agtcattaac tgaaagaaaa
    17161 ttgtttgaag agatttgtat tattgaatta gaaaaagata aatcaagaga aaagggagat
    17221 atgaaagctt ttaatgattt atctaaatta gtatcaagta aaatgcaaga tgctgaaata
    17281 aagcctagtc agaaaaagaa agctggagat tcgcaagatg atacatttgg aatgaaaatg
    17341 atgatatatg aaaaaaatca acctgtaaag gataggttaa aggaatatga agatgtagat
    17401 ggatttgaag cttatgtaaa taaacatatg aaaaaaccac ttgctgttgc attaggttta
    17461 gctacaggta agtattcgat aaatgatggc gataaagata ttaaatttaa agatgatgta
    17521 agagacattt tagaaggtaa taaaaatgag gactaaatat gaaaaaagag agctgtcatc
    17581 taatgattca tcccgaaatc tactagagag tataggagag tattggggtg ctttttatct
    17641 cgcaaatcca catagattct gtatggatta ctttggattt aatctacatt tatttcaaca
    17701 gatattaatt tatatgatga tgaagtctga ccaatttgta tttattgctt ctcgtggact
    17761 gggaaaatca tggcttctag gagtgttttg ttgtgttata gctgtattaa aaccaggtac
    17821 ttgtgtttta atagcagcaa aaagaaagaa acaagcaaaa ctacttatta caagtaaaat
    17881 tttaggtgat ttatatttaa aatcagatac tctgaaaaga gaaattaaaa gtttccaagt
    17941 aaatgcacaa gaagtatcta tagatttttg gaatggaagt agaatagaag ctgttgtatc
    18001 taatgatgat gcaagaggtt acagagctaa tgttcttatt gttgatgaat atagaatggt
    18061 ggatgaagga actgtgaatg atgttttagt tccattttta acaaacccaa gacaaccagg
    18121 atatttacag aatcctaaat atagatatat gcaagaagag aataaagaaa tttatcttag
    18181 ttcaggctgg tattctcagc attggagtta taaaaagttt atggaaacag taaaaggtat
    18241 gcttagtggt gaagatatgt ttgcatgtag tattccattc acttgctctt tagaacatgg
    18301 actattaact aaaaaaagaa tacttaaaga aatgaaaaaa gagagtatga gtgatgcttc
    18361 tttcatgatg gagtattgtg gagtgtttta taatgaatca gatgatgctt tcttcaagtc
    18421 atcttgggtg aatccatgta gggtattaga aagtatgttt tatcctccta gtgatataga
    18481 gtatcttgaa aataagaaaa aaagagataa gaaatatcat cttaataaga taaaaggaga
    18541 aatacggatt attggtgcag atatagcttt ggccagagga gttaagaatg ataattctat
    18601 ttatactttg atgaggatgc ttccaaatga aggcacatat aaaagatgtg ttgtccatat
    18661 agaagcttat aatggtatgg aagctgaaaa acaagcaata agattaaaac aattattttc
    18721 agattttcag gcagattata tgatattaga tacacaaggt ataggaacaa ctgtatggag
    18781 ctatatacag aaagcaaatt atgattcaga tagagatgaa tggtatgatg cttacacatg
    18841 ttttaatgaa gataatactg ttgacaagtc actagcaaaa aaatcacttc ctgttgtata
    18901 ttccatgaaa gcttacgctg atgaaaatca taaaatggct atgtctttaa gagatgtcct
    18961 tacaaataga accttagaac ttcctataag tgatattgaa gcaaaagaaa tgatattaga
    19021 aaaagaaatg ataaaggcag atgaaataga taaaaaggca gaattagaag caaaatatat
    19081 tgccccttat cttcaaacaa cggctttagt gaatgagtta ataaatttag aatatagtgc
    19141 tgatggagga aaaattaaaa taaaggaaaa aagtggagca agaaaagata gatattctag
    19201 tttggcttat acgaatttct tagcagatta tctggaagaa aaagagaaaa gaaaaaatag
    19261 aaataatcaa aaaactgtta tgatttattg gtaagagatg aggtgaataa gtgggcaaaa
    19321 aaaataataa taaaagcaag tctaaagaca acaattctac acaaaatcaa atcaatatgc
    19381 taaatgctca aattggaaaa tatgcctcag taaaagaatc cacaacatta gcaacagaaa
    19441 tgtataagtt aagaagtata agcagagaca aattaagaaa agctctctcc aatccatatc
    19501 aaatttcaaa tacagatata cttcaggatg caagtatgat attaaaagct acatctggta
    19561 catatagaag agttttaaat ttaatatcta atatgaatac ttttgaccat atcttatacc
    19621 caaaagacat aagtagatta aaaacaaaag aaaaaataac aaaagcatat atgaattcgg
    19681 cgagtcaatt agaaaaatat aatataaaat ttacatcagc ttggataacc gaaaaagtat
    19741 tggaattagg agaggtttat ttatataaga tagaagataa ttcagggatg gttttacaac
    19801 agataccagc aaaattttgt atgataacaa tgatagaaaa taatgtaatg agatacgcta
    19861 taaatataaa aaaacttact gacaaaaaca taatatcctt tccagaagaa attaaaaaca
    19921 tatataaaaa atataaatct aatttactta cacgagaaga attaattgat aatacatatt
    19981 ttcaattaag tgataaagca gtagcattta attatgattt agattcagtg aaaggagttc
    20041 catttttttg ctttatattt gacgatttaa tggagcttga agatatgaaa gatttaaaaa
    20101 gcacaaatgc agtaatagaa agtattaaat taatacatca aaagatacca tttggcaaaa
    20161 atgatgaacc actagtagac cttaatttaa ttcccatata ccacaattca acaaaagcaa
    20221 atttaccaaa aggaacagca ataactacaa atcctttaga gttggaaaca catactcttt
    20281 cagatggaaa atctaaaata aatgattatg taaaagaagc aaaagagttt atatttgata
    20341 acgctggaat aaatacagcc ttacttaact ctgacaagat aaataatgaa tctatcttaa
    20401 atggtattat tgcagatagc ttaattccta tgaggattca acaaatgata gaaaattggg
    20461 ttaactttga actaaataaa gacaagaagg caaatttatt taatatgaag tttattggaa
    20521 ctactcactt taataaaatg aatttatcca aacagtatcg agaagatatg ggatacggag
    20581 ggagtaaaag cttgtttatt gcaagtactg ggtttactcc acttcaagct ataaacactt
    20641 tacaagcaga aaaattaatg ggatttgatg agtttttaat acctcaacaa acatctcata
    20701 ctcaaagcag tggtagacca gataaatctg atatagggac agataatggt aattctactc
    20761 aagcaaaagg aaatggggag aatgattaat tatgagtaag tttatatatg ctttttctga
    20821 agatgataag aaattattaa tggaaaaagg atatcgtttt atatgtgaaa acaagttgaa
    20881 taataagact ttatatgttt ttgaaaataa atcaaaatta ataaataatt tcagtaatga
    20941 agaaatgaaa agatttatat ttactagcaa aatacgtttt taaaaggagg tgaaaaaaat
    21001 aaaaatatta aaattacctt gtaatttaaa acattattca gataaaaaaa aattggaaga
    21061 actaaatagt aatcttttgc cagtgtatat ctatgttatg catgaaggta caaatccaaa
    21121 tggaactaaa ttttatgaag aagctattga taaagctgaa ccaacattaa aaaatgttcc
    21181 aattctagga tatgtaaaaa taaatgaaga tggaaaatat gactttgatg gtcataatgt
    21241 tttaacacaa gtagttcaaa cagatgaagg gtttatatta gaagaatatt atgaagaacg
    21301 aataattggc gttataccag aaacaaataa atatgaaaaa gttgaaattg atgggcagaa
    21361 gtatgttaaa tgtaaaggat atatatataa atcatatagc aatcatgctt acgatataat
    21421 tatggattct gatgaaatag aaatttcaat ggaaatagat ataaatgatt atcaattaga
    21481 tgattcagat ggattttata atataaaaga ctatgtatat catggaatta catgtcttgg
    21541 ttctgatgta aagggagcta tgggttcaaa ttgttgttta actaaatttt caagaaaaat
    21601 taattataaa gaagaaatat caaaaatatg ctcagaaatt tatgcattag aacatggaga
    21661 gggggaaaag aatttgccaa ataagaatca aaaacttaat aaaaattctg aaggatatgc
    21721 tttagcagtg agtaatctta gtactgaaat aagaaataag ttaaaagaat acaaagtcga
    21781 aactgagaat tggtatggtg aaaaagttga agtacaagct ttttattata atgatttaat
    21841 accagaagag aatattgcta tagttgaaga tgaaattaac tgggggtatt actatggtat
    21901 tccatatata gttaatgaag atgctgtcat tttggattat gaaaataaaa aatcatatat
    21961 acaaacttgg agggaaaaac aagaaggaga aatagtacag gtttttagta gacaagaaaa
    22021 attaaaaaaa gaaataatag agaaatttac tgaaaagcaa aaagaaatat ctaatttaaa
    22081 agccagttta gagccattac aggcattcaa agaagaaaaa gaatttgagt tatttaaatc
    22141 taaagttgat gatgtagctc aaaaatttaa attagcagaa gatgaaatta aagatataaa
    22201 aataaaagct tataataagg aaattacatt agatgaatac aagaaagaat tgggttacat
    22261 atttgcatta aaaactctta gcaataaaca aagcgaaaag gaaaatttta gtataaatga
    22321 tgataaaaat aatacgataa aaatacctat aagtaataat aatgatagtt ttagtgaacc
    22381 agaagaaata tcttttataa aaaaatattc agataaagaa taataggagg aatttacatg
    22441 aagaaagata aagcaataat acaaacagat aaagtaaaaa atcctgatgt tttaacaggt
    22501 aaatatgttg ttccatcaga ggataccaat ccaataaatt tagaaaatgg tgctgtaata
    22561 aatattggag aacttgaaga cacggagtat ggtagggata cccataaaat atataaagtt
    22621 acaaatgaca cattagattg gggtatagtt gatgacccag caactatgta tgatgaaaga
    22681 ttagatgaaa gagattacga agtttctcct ggtcagatat gtagatgtag acgactaaaa
    22741 aaaggagatg aggtaactat atctctatta catatagcag ataaaagtat tgcagtaaaa
    22801 gacaagttac aattaaaatc tgattcgttt cagtttgaaa aattgcctac agcagatgct
    22861 aaaactccag tagcagaagt attagaatta tggaactatg aaggtcaaga ttcggttaca
    22921 ataaaagttt tataaaatca aataaaatta acaaacagga gataacagtc tataactgtt
    22981 atttttttat gcaaaaaaat agaaaaggaa gtgtgtaaat taatggcaac agcaaaaact
    23041 ttaaagagaa tagctcatga gctatataca gatacattta gagaatatac agatagagat
    23101 ggaaacacaa taacatatag agatgctgaa agtgcaataa agaataaatt aaaagaaatg
    23161 atgggaggag aaaaatataa ttattataaa ttccaagaaa ataaatgggg attttataat
    23221 ttaatatcag aattaatatc tgatgatatt aatagattaa atgaggaagt atttagtccg
    23281 ttttgtgatt ttgagaactt tgatttagga gataaaaaag aatttactgt tgaaaatact
    23341 aatttattta aagttgcaaa tatagcagat ggtgttaata gtacaagaag acaaagatta
    23401 cttaataaaa aagttcctac aacagcattt aaattatctg ttgctatata tgaagaagca
    23461 gagagattta taacaggaag aataaattgg gttgtatttg tgaatagggt atcagattca
    23521 tatcattatg atattgctag aagaatagcg aagacatttg aaggtgcata ttcaacaata
    23581 aatgctaagt ttcaagctac aggtaattca gacaaagtat tattggaatt gatagctaag
    23641 gtagaagggg ctactggtaa aaagcctatt atatatggta ctccacttgc attaagtaat
    23701 ttagaaggtg ttcaaactga tttagaaaaa gaagaaaaaa gaaaatatgg ttttatacaa
    23761 acatttagag gtggatataa agtacttagt ttaccaaatg cttatgatga aaatgcagca
    23821 gaaggtaaag agtgggcttt agataataag gctatctatg taatacctga tggagagaag
    23881 ataatcaaac ttggtactga aggagatgta ttggtcatag aaaatactga tgaaaaagaa
    23941 agagatgacc aacaaataga atattttatg gctcaaaaaa tacatttagg agttgtaact
    24001 gctgctaaat ttggagttta taaaatacaa taattgaatt agaaggtggt tatccacctt
    24061 ctttttttta tgggagggag attattatgg caagagctaa aaaagaaaat atttctaaaa
    24121 cagaagtcaa agctaatact ttagaaaaag atataaagtc tactttaaat aaaaaagaag
    24181 ctagattaaa ccgattagaa ctacaaagaa agctaaaaca aaaaaaacag gaaatagata
    24241 tagaaataaa aaatatatcc acatgggatg ttgattatat tgacccaaga gaaaaacaac
    24301 aaattttttc tttatctaaa gtaggttcaa aagattcaac tgaatttata gatttagata
    24361 ctctttatag aatagtgaga agaagtccag gtttttttga agaacataga ttaataattt
    24421 cggatgtaga ttctttagat gttgaatgta ccccaatgga cataatagat tttctaggat
    24481 tgaatagttt atatgaatat ataagaaatc ctaatgaaga ctatttagaa tatttcttat
    24541 ctgataaagt tgatataaat tcttttgaaa aaatattaaa taaaaataat gttgagttaa
    24601 caagaagatt agctgagagg gcaatagatt tacataaaaa gaaaaagttt gattctgggc
    24661 taaaggctaa actattagct aaaagaattg gaatggaaga tttgtacttg tttagttaaa
    24721 ggtggtggaa tatttgggta caccacttgt aaaaatatat aaaaagtttt tggatggtat
    24781 taatgatgaa gagatgttat tactatcaaa tgaaataata gaaaaaatga tgtacagtta
    24841 tttagaagat gctatagttg attttaatca atgtaaaaaa gatttgacta taaaatatgt
    24901 tgatgaaaaa ggtggagaaa ttattcctgc tgctcaatta agttatacat ctaattatag
    24961 taataaaaat gcagagataa ctttgatggg aaaagataca aaagaagaat acgaattaga
    25021 taaagattat acaataagct ttgaagatga aaaatttata ataagttttg ttgtagaaac
    25081 tacagaagag ataattttca aatacaaata tttaggagaa atagtatctg atttagattt
    25141 agatgaaatt aaaatattat catatggtat gcaaatacat tggttacaac ctaagataaa
    25201 tagagaagaa aatttaaaac agatgctaac tgattctgat tataatgcta aatcaggtgc
    25261 taatatgttg gctaaacttc aagcaagaga agagcaattg agaactagat ttaataaata
    25321 tcaacaaaga tacatgctaa aaaattttga aggatggaac tagcatgagt tacttaagtg
    25381 atacaaaaaa aagaattgga cttggttgtt caactcctaa agaaaaaaga atattacaac
    25441 ttagattaag ttttaagaaa tatctaaaag aaacaccaac ctgtattgaa gtaccaataa
    25501 ctgatataga tgaaatttgt ataacagaag atactaaaag agctatagtt gcaattaatg
    25561 atataactaa taatgataaa agagctttag atgaaaaaaa tctattagtt gaatctgatt
    25621 tagatgtaga tgtgggttgt tatcttttct atgataattg ttattggtta actatattta
    25681 aagaacataa agaaatggat acatacaaac atttcataat aaaaagatgc aatcagtttt
    25741 ttaattataa atataaaggt cagatgtata aaatccctat agctgttgaa aatttaacct
    25801 tatactctga tggtatggca gataataaat atacttctat ctcagataca aaaagacaac
    25861 tttattttgg aagtaatcct gtcactaaaa ctatagatat tgacactaga ataatgttaa
    25921 ctggtaaaac tgtatttaga gttactagta ttaatgactt tgagtataat ggcagagaaa
    25981 caggagcaga tggtcttatt aaagctattt gtttacaaga tgcattgatt tcaaaagatg
    26041 atacaataaa taatgttgct tggaatgatt tatctgaaaa tgatagtgta attattcctt
    26101 tcagtaagat tataggggat ggatttatta atctaggtga agaaaatgaa tacagaatag
    26161 accattccaa aggagttaaa tggctcttag acaagcaata taggtattgt aatataatta
    26221 atcaagatga aaaaaaatgt gtcatacaag ccaatatact agctaagtat tctggattag
    26281 aagtattgct attagctaaa gataaagaaa ctaatgaaac aatagatact aaaaaaataa
    26341 cactaagggg gtagatatat gggtctatat ggctttccct aacaagatga taagtaatat
    26401 aggaacaact ctaatgtcaa atcaagactt tgctaaattt atgatttata atgacgaatc
    26461 ccaaaaagat attttgtcca tgcctgatgt aaaaaatcct gttaaagagc ttaaaaataa
    26521 aaaagtattt ctaaatagaa gagtagaaaa agtgttaaaa gatgcagata tttcagtttt
    26581 tataataatg gcagagtata gaccatgtac tactggaagt agagaaatta aaaaaacaaa
    26641 gatagaaatt ggtgttgttt gtcatgatga atgccaaaat acagcaaatg gcttaagaga
    26701 tgttgcatta gtatgttgta tagttgatat tataactcaa aatgaggaaa tagctggtat
    26761 aggtaagatt aagttagaaa atgtatatca aatgtataat ttaaatactg attataacgg
    26821 atttgttata actgtatcat ctgaaagttt tggtgatatg taatgttgga aaattattat
    26881 attacaggat taccaattaa gctaagtgaa acattaggaa ctgtatatca acctattatt
    26941 gaagaactta ttaaatttga tatggctaat ttagagatag ttaatccatt tttagtactt
    27001 gaaaagagct attctcagct atgtaacgag gaatcatttg aattgaaatg taaatatgat
    27061 gctataccta tcttagattt gatgatgtta acatcaagaa aagattcttc tgaagaaatt
    27121 gagttactta gtgataagat aaaaaaatct ttatctatat tatataaaac tgatattaaa
    27181 aatattgaat atatgaataa gattaaaatt ggtattttaa ttaaatttga tgataaaaag
    27241 aaaaatgctt ttataagtag agaggatttt ggactagtat cagatttgat tttagagatg
    27301 ttttgtattg ataaaaagaa tttatttaaa gatgatgaag ataagtggat tgaaaatact
    27361 ggctctgaaa gagaaaaaca gttgattgca cactttaaag agaaggaaag aaagaaaaga
    27421 gaaaaagaag catatcactt atgtgattat ataaatgtag ttcaaaacat agatggatat
    27481 gtaccaatag atgttatttt aaaaatgact tattggcaat taattaatgt ttataagact
    27541 aagatacaat ttaaaaacta taatgaaagt ttaggctatg catggtcatt taaatatcaa
    27601 acagatatgg ataaaatgaa gcattggtct aaagagataa aaataccact tagcactgtt
    27661 aaatagcagt gctatttttt atgcaaaaaa tgagaggatg attttataat atgaaaagat
    27721 ttgcagtgaa agatgcaggt aatgtaatag taaaaaataa agtaacagga gaagtattgt
    27781 tttattcaca ggatttaaat gcttttaatt ttaagatgga ttcagaatca gtttatgcaa
    27841 aagctaaagg tgcaaataca atagcttttg atggagcaat aacagcaagt ttaacaatgg
    27901 aacaagaagt aatacaaatg gctcaattag cgatgttatt atcttctgat atagatgaaa
    27961 aaactgcaaa ggttgggaaa agaaaagtat taacatctga tagtactaag aaagttactt
    28021 tagaaaatat aaagccagtt gcaaatagca tatctgtata tagtatagaa agtgatggaa
    28081 tatctataat taaaaaatta caattcactt catcagttac aggagctaat acagaaataa
    28141 caatatctac agccgatttt aatgcgggag ataaagtagc agtattctat ttagaagaaa
    28201 taccaaaagc aaaagttgta aaaataaaag aagaatctac tgcaccaaat tacgttgtag
    28261 aagctgaggt aatggttaaa actgtggatg gtgaatatat ggttttatac atgagtgttc
    28321 caaatgcaaa agcacaaaga agtatagagt taaatcttac tgctgagaat ccatctggat
    28381 ttaatatgac attagatgtt ttaccagatg aaaataaaga gtatgctaca ttcacattta
    28441 taggagatga aaatcttaat cctgctagaa tggcttcaat gctaggtgtt gaattagaag
    28501 atgaaaaaga tactaaacct aagaaatagt aaaatgccct actcttaatt gagtagggat
    28561 tattttttta cgtttaaatg tggttttaat cggatttgaa cttaaaaaaa tgaaagaagg
    28621 tgataaattg gtatttttag atgataagtt tttatttgat aatgtttcaa gtgaatctat
    28681 gaacatcaaa ttagttacat taggtgatga tgaaatactg aatgaatatg gattgcctta
    28741 tgaagaagca attaaatctg atagcagttt taataaaaat ccttgttaca gtgaagatga
    28801 aactactata gaacctataa cattacaatt ctgtttatgt agcgaacatg gggaggctta
    28861 tgaatgggat gattatacgc tagaaaacat atgcaattgg ttttggcaga gagaatttaa
    28921 accttttatc agttatgaca atatagaaga aatatactat ttcaaagcga gaaaaataat
    28981 aaaaagatat acaaaagata aaaaaggcgt attagaaata gagtttcaac catatacaaa
    29041 ttatgcttat agaagctttc aaaaagttat aactgtcaag gatacaaggg aaattaaact
    29101 aaataatgtg tctaatgtag atgaagaata tgctccagta attgatattg aatgtctaaa
    29161 agaaggagat ataactatta gaaattctac tatcagcgat aatgaagaag acaatttagt
    29221 tataagtgga ttagaattaa atgaaaaaat aacaatagat aatttatact atactgtttt
    29281 aaatagtaat ggagagaata ggtttaatat tattaataga aagtggatta gattaagaag
    29341 aggtgtaaat atattgaagt ttactggaaa ctgtaaagtt tctattaagt gtaaatatcc
    29401 aataataaaa taagggagag gtaaacatga ataaaataca agtagataaa ttaatacaag
    29461 atgaagttag ggctataata ccaattatag atgagaatgg taaagaggaa tatatagaag
    29521 ttagaaatcc tgataaggag actaaagaag agatattaaa taaaatatgg gttggtatgg
    29581 agaatcctga tttagcattg tctcaagaag atattcttaa aatgttgatt gataaattga
    29641 ctaatataga gttaaatatt gaaatagaag atttagtaga tagcaatata tctagcgaat
    29701 tagaaactgt gatgtatcac atatgtcaaa taggaaatga attaactgca tctttattaa
    29761 tgaacactga tgttaaatta ggtcagatga agaatgagat attacaagac agggttttaa
    29821 aagaaattga agaaactgaa gaaattgaaa aaatgaataa tattaaagac aaggtggtga
    29881 attagatggt atttaaatca ttggatgagc tagttgctta tacaaaaaat aagattgctg
    29941 catctatgcc agaagttgga aaagaaatga aagaaattat gaaggaagaa gtaaataaac
    30001 aggtttattc tggttatact ccagttggag aatggaatcc taatgtttgg ggaggtagaa
    30061 caggacaatt attagatgct atagatgtta cagaactctc ttctaattct gtcacttcag
    30121 agatacaaaa taatggaaat tgggtggatg catttacacg taattcagca tttccaatgg
    30181 aaagattaga aaaggggaaa gtctggggtg ttggcggata tagacctcaa acaaatataa
    30241 tggaagagag taattctaaa gcacaagaga gagtacctaa gtgttttaag tctaaaatga
    30301 atgaattagg cgtaccagta aaatagataa tatcgagtgt ctattaagtt atagagtttt
    30361 ctaagggttg tatgttatcc actagtagat gtatatcata agtggaaaat taaaaagcgg
    30421 tgatgctcta acatatagtg agcaatttaa cctagatagg tagtttagtt gatttgctta
    30481 gataagcggt ttagaattgt ttctcaatgt tctaatacat tgattgatgc aaaaacatat
    30541 acaagtatat tgtgaaatat acttgtatat aacatattga tataaggaga gaggtataat
    30601 tgttttaatt aacatttaaa tttgaataca tcgaggaagt attagaggtt acaagaaaat
    30661 tagattatcc taatatacat tttagggaat cttgtggtaa gataatagaa aagtaagaat
    30721 taaaaaaaga tagttttaca aataataaag tcaatattcc aaaatattta atatataatt
    30781 atgatgatga tatttggact ggagacataa taaaaatggc taatcaggca aagaaaaatg
    30841 attatagctt ctttattgtt taacaatata atttattttg tagatgacga atatagaagt
    30901 tataaaatag gtcttataga ttaaattgta aaatttataa gacgtttatt attatattaa
    30961 tatagataaa gcgagtgacc atagtacacc tttaaggtgt ggagatagaa taaacctttt
    31021 attattataa gataatagaa ggtttattct attgtaatat aaatatcaag atgtattaat
    31081 aaatattaaa gataatcaat ttagattgtt ttttaaattg aatcatttat gttgtagtta
    31141 tttcaatgat gacatgtgtg tgacaagtat aatacacttg gaaatattga aagatatata
    31201 ataccttaat tgcttagaat cgaacataag aggttgattt ttatggttga aagtattgga
    31261 atttcaacaa gtgttcctga gaggaacatt ttgtttggtg tttagttgtt ttaatgatga
    31321 cataggtatg acaaagataa ttatgtttat atattataaa taaaaataag catctctgat
    31381 tgttgagata cttattttag gttgttttta tcttttatta gtttatctac ctacttatcc
    31441 acttaaaatt aatgtaaaaa tatgttataa tatagcttgt atattcatat ttaaaataag
    31501 gggattttat agtggggaaa atatataatc tatatttaga tgagagtgaa actcatgatg
    31561 aaaaagatgg taattggata aataaagtat tttgtatagc tggaataata gtggaagata
    31621 aatatgattg taatgagcta acaaaagatt taaataaaat aaaatatcaa atatggaaag
    31681 ataatataaa ttatgaggct ttaatattgc atgaagtaga aataaaacaa gctaataatg
    31741 gttatgataa aaaaaatcaa aatttagtaa aacctcacaa taggattttt agaaaaaatc
    31801 aaaacgtgaa aatattgtat agaggtttag aaaaaataat aaggaataaa aaaataacga
    31861 cattagcagt atgtataaag catgatgaac ttgataagtt gtacaataaa gaaattcaaa
    31921 acgatagaac attaatggct ttacagatat taatagaaaa ctatgttcat tttttgaaaa
    31981 ataatgattc taaaggtcgt ataatttatg agcataatgg agattctaat tctaaaataa
    32041 tgaaaatgaa atttaatatg attaaaacaa tggggacact ttttataaat ccatatgcta
    32101 tacaggagag attgttagac attgaatttc caagcaagga tgaaaatata gcaggattgc
    32161 aaatagcaga ttttattcca aatataatag ctagaaaagt ttctaataaa aaattaaata
    32221 aatataatat ttataatact atcaaaaggt ttagttatga tggtaatgta aaaaataaag
    32281 ataaatttgg gataaaaatt ataaaattat agttgtagga atattgacta attataatat
    32341 atatattata attatagtaa ggattgagga gctattactt gcgagttaat gatttgctgg
    32401 aatggttttt catgtgaatt gattattatt aaggattaat atgatattcc atagctccta
    32461 actagcctta agtttaaaat ttataaatat ttaaaaacta gataaattat attttgatat
    32521 aaaagataaa gtctatttaa agtaggcttt tttttgttat aattaaatga tgacagaggc
    32581 atgataagta tacatttaaa agatttttac atataattct ttattttaca aatatataca
    32641 attagtggag gtaattctac atgtttcctg ctataattat agtgttagta gaataagctt
    32701 tgagggacag taaagctact gtaagcttat gacagatatt atagggggta atttatatga
    32761 gtgaagatga aaacaaagta tcacaggaaa atgatagtac taaattttgt atatcatgtg
    32821 gagaaaagtt attagaagga caactttttt gttctaagtg tggtaagaag gtagatgaaa
    32881 agactgtaat aagtaaaaaa gcatcaataa ataaaaattg ctggtataat attgatggtc
    32941 atagtgggga ttttcaccag tatcaatatc aaggaaaaaa aacaacaaga agctagggcg
    33001 aaatacataa tgactattaa agattttcaa gaaaaaagtt atgtagcagg tgctaattta
    33061 gaagatattg ctgacactat tcaaacttat tggaaagaaa atatttatga aaagaaacat
    33121 ggttctaata ttgatgctgc tattttcgct gcaatgttgg ataaaagtga tgaaatagag
    33181 aagtcaaaaa aatatgatga tgaacttcta aaaatatata gtaaattgaa atctgttcct
    33241 gaaggtagtg aggatttaaa tgatatttta agtacaatag aaaaattcta taattcatat
    33301 acagattttc atagttttgc aactgagcca agtgggagtt atgtagatta ttctgctagt
    33361 aataacacta aaacagatga atttctatca aattatagag ctttagagaa ttcaataaaa
    33421 tcaaatagtg aattaaacaa ggctaagtct acttcagaaa ccatgatgat gaacattgaa
    33481 aaagcaatga aagagtatga gattatttca gattctattc ctaaagatat agacccttta
    33541 gatgaaaatc tttatgaaaa agttaaaaag attgttcctg acttctattc agataaaaat
    33601 attgatgtaa gttatataga aaataataaa ggaaagttta aaaatgatga aagagttagt
    33661 aaagcattag accatttttc agcagctaag acttcctatg acaatatggt taaatatgct
    33721 aaggcatata aaaaatctaa ttctcaagat gatttggaaa aatttaaaaa tgaaatattt
    33781 gactttagag catatgtagc tatatatggt cttcaagtag aatattcaga tgaagagaat
    33841 taatcagata agtaaaaatt attatatata aataaaaggc agtcagttga ttgtcttttt
    33901 taattgagtg atttgtatat tagcgatttc aatagtgaca ggtatatgac aatcataatt
    33961 ttaaaaaaga ggtatatatt aattatcctc ttttagtctc tcatatgcat tatctatcat
    34021 tctatctata gcttttctat ccatttcatc tagttgtata tattttttaa gaacttcttt
    34081 gacttcgtca ttataattat tattttctaa gaatttattt atatcttcta aagacttaat
    34141 gtcttttttt tcatcaacta tatcatagat ggaagtatct tcgcatatga ttgatggcat
    34201 accaggtata tttgcactga ctgaatagtt ggtttttgta tgacctaaaa gataatctgt
    34261 agtgacatta aagtattcag ctagtttaac tatcaattct ggagatggag ttcttttatc
    34321 attttcatac atacctacag ctccttgtgt tacatctaat attcttgcca aatcggattg
    34381 ctttaagtta tttttaattc taaggtgttt taatttttct ccaatcatgt tctaagacct
    34441 cctatacata tattataaca caaaaagtta taaaatatat acttgacata acaaaatgtt
    34501 ggcaatataa tataaatata acaataagta ttataaaata ctaaacgtta taaaaataaa
    34561 taaggatagg aaaaggggct attctcccct atcaagtttg aatagcccct tatgatatat
    34621 aattaaatta aggtgatatt gagcatatta caaaaaatta aatagcatat tgactttttg
    34681 tgtcaccaat aatataataa atgtatcacc aataagtcag gaggtttgga atgacaccaa
    34741 gaacagggag acctccatct gataatccta aaaaaaatga atatagaatt aggatgtcag
    34801 atgatgaact tgagatactt aatcattgct gtaaagagtt aaaacttact aaggcagatg
    34861 tagttagatt gggtatcaaa aaagtttatg atgagctaga aaaataagga taagaaaagg
    34921 ggttattcgc ccgaccaagt ttgaataacc ccttatggta tataattcac cctaatgata
    34981 ttatatacca ttccttataa aaaatacaac ttataaggag gtattaatta tgggaataat
    35041 taatagagaa aatgtaaacg aaataatgag ttttgaggaa tctgaattaa gtaagaagtt
    35101 tgaaggtatg gatgtagaaa taattgaatt aaatggagag ttattgtttg aattatattc
    35161 tactggagct gcacttggtt atactagaaa taatccaagt ggtggtaaga gatatcttca
    35221 aataagaaga gatagaattg ataatgttat tgaaaaggct gaaattaagc cattggaccg
    35281 cagtggtaca aaatatttaa ctgagcctca actatacgat tttatgctag aagctaaaac
    35341 agcaaaatgt agaccattca aaaaatgggt gacaaatgaa gtattaccca caataaggaa
    35401 acatggagct tatatgacta atgaagcact agagaaggca atcaatgacc cagattggac
    35461 aatacaacta ctcacagagc taaagaaaga gagagctact aaagaaaaat taagagtaga
    35521 gcaagagaaa aataaaccta aacttgaact agctaatgct atagaaagtt catcaagctc
    35581 aatactaatt gctcaactat ctaagatatt aaatcagaat ggagtagata tagggcagaa
    35641 tagattgttt gagtggatga gaaataatga atatctaatt aggaagaaaa gagctgacca
    35701 taatactcca actcaaaagt caatggattt gaaagtatta gaggtttctg agagtactgg
    35761 gatagataaa gatggaaata ctgtaataag acaaactcct gttgtaactg gaaaaggaca
    35821 aatatatttt gtaaataaga tattgaaaga gaataacaaa gtacttaaga gattcatgat
    35881 aaataatgat tacataagag gagttatggt tgagataggt aaaacatatg ctatatatga
    35941 ccatgagtac tatggattta ttggtatatt aaagagtgta agaaatgaga aaataagagt
    36001 agaaaaaata ggagatgttt ctagcagtag ggatattcca atctgtatga tagattacat
    36061 agaagaagct agtaaagaag atattaataa atatttgagt aaatgatttt gtggaaatag
    36121 gagttttggc ttctatttcc ttttgtagta taaattttcc tttagaattg atacaattaa
    36181 tgtatcagaa taaaggagat aatgttatgt taataagtga atatattaaa gataatagat
    36241 gtgggaaaaa aagtaaatta tatttcaaaa agcttgaaga aataaaacaa gaatttatta
    36301 atagagattt agaatatttg ttgttaacaa cttgggacga agaaatctca tgtatgatga
    36361 aaactggaag agatttggat aagttaaaat atagggatgg atttaaggaa ataaacaatt
    36421 tatttgatag catatcagaa gaaataagag atgaatataa agaaaaagat acatatacaa
    36481 tgttttttag cagttgctct aaagaaaaat attatatagc ttgtgagtta gccaatagtg
    36541 cttataaaca tataatactt cctaatatat ttgaagatga caaaagtgta aatcacaaag
    36601 ttgtgtttaa aaaagaacaa aaaaaagaaa tagcattaat gttgaaactt atttctagct
    36661 atatgcaaca tgggttttca ggttataaaa tttatgatac agatataaaa tttatagaaa
    36721 atctatgttt gtttgagggt aaaaaggttt gttatccaaa accaaataat ttaacacatt
    36781 ttttaggaga tatttctagg tctgataatg acccaataga aatacttcat gctagaaatg
    36841 taataaacaa aataatggaa aaagggtatt ttgataatga atttgaacta gtgtattttt
    36901 taagagggaa aataatgact ccaatgcaaa ataagtatga gaaaattgat acatcatcat
    36961 tagaaaaaat aaattattta gaagaaaaag cgcttatcca gaaaaaaatg attttaaatg
    37021 atgaaatggc ttataaatgg aaaagtgaga ttaatatgtt taaagtaatt tgtaaaaagt
    37081 ttaaagatgc aacacatcaa tatagacctg agtggttaaa tggtcaaact ttagatgtat
    37141 ttgttccctc tattaatttg gcttttgagt atcaaggaca acaacatttt aagagtgtag
    37201 atttttttgg aggggaagaa actcttgaaa aaaataaatt aagagataaa aaaaagaaaa
    37261 ggctatgtga tgaaaataat gttaaattaa tttattggaa ttatgatgaa ccaataacta
    37321 ctatagtatt aaagaaaaaa ttaaaagaaa ataatgtcga attgtaataa ttttcctaat
    37381 agtatattct tgatataatt gaagtatcag gataggggga gatgttatgg aagaatattt
    37441 aggtcatttt attttaatag ggctaggtgt aggatatatt attgtttatt gctttataag
    37501 aaacgtaatg gataatgcta aagaaaaaga agctagatta gaaagaatag cagaaagaaa
    37561 tagagaaaaa acaaaggtgg aaaaggctat acaaaaaaat atttcaatgg ctaaatgtca
    37621 agcttttggt aattcaaaca gtgacagtgt gatattaagc aataatggta aaatatctat
    37681 cattcatgct ggatttaaga cagcagttaa aaaaaatata gaagatttag taagcattaa
    37741 gtttagcatg caagttagtg aaaaaaatca gatgaggata atatctatag ttcctacata
    37801 tgataaatat acttttgtag aaaaaatata tttaactttg atatttgaac ttaatactta
    37861 tgaagtattt tatattccac aatataaaaa tataaataat gcagatattc aagaaaaaat
    37921 aaaagaaatg gaaagattta aattagttgt agagaatgaa gcagccaaat ttaaaaatgc
    37981 taaaatagaa agcaataatg atataaaaaa agatgatgat gatatatcaa aaatttcaaa
    38041 ttcattaaaa gaattacaga gtttgaaaga ccaagagata ttaacagaag aagaatttaa
    38101 tgaaaagaaa aaagtattat tagagaaaat taaataaaaa tattaagaca gtatattaac
    38161 tgtctttttt tatgctttaa aaaggagttg gttaaatggc agaagaattt aagattaaga
    38221 ccagtataga gcttgatgat aaaaaagcta ggaaacaatt agcatcactg aagacatctg
    38281 caaaagaaaa taatataaag ttaaatgtgg agatgaatgc atcctcatta aataatttaa
    38341 agcaattaga gaacacatta aaacaaatta ataaacttag tagagaaacc caaaatggat
    38401 tatttggtgg aaaaggtaat tcaaatggga atataaataa attaacttct caatacgata
    38461 actttaggaa aaaaatagag tctactcaaa gacaattaaa gaaatttact cagacaaata
    38521 ttttagacaa taagcaaata ggagaaatta acaaattatc aggagaattg aaaagattat
    38581 ctaatattaa attaggtggt ctaaattcaa aggctctatc agatttatca aatgttcagt
    38641 ctaagttggc taatatgaaa ataccagata tgaatacaaa aatggcttct caatttaaaa
    38701 ctcttcaaaa tgaagctgga aaattagcta ataaaataga aacattgggt aagtctggat
    38761 atgcagatac atctaaacta caagctttat caaattcatt aaagtcaatt caaaatttga
    38821 atcttaaaaa tctaacttct aatcaaatac aatcagaaat acaaaaacta gaacaattaa
    38881 aaaataaagt taaagaagta gaaaatgcta ctaaaaatac taaattagat gctaagttca
    38941 atatgaatct ttccaaagta atttcagatt taaataggct tagacaaaaa tgtttagaat
    39001 tgggtcaatc aactgctgga attgataaac tagaaaaaga attaatgcaa ttaaatggta
    39061 tgcctctagg tcaaaaagta aaggaattag attcaataaa aagcagatta agcaatatga
    39121 aatctaattt cacaggatta ggaactagtg taaaagcaac aaatggattt tttagtgacc
    39181 tatataattc gatgagaact tatactttag gtaatatgat aggtatgtct attacaaatg
    39241 gtgtaagaaa tattaaaaca actatcgttg aattagatag tgctttaaga gatatgatga
    39301 aagtagcacc tgataatttt gaaggtacaa gtgagcaatt aaagaatgta aaaaatgaag
    39361 caatatcaat agggaaagat acagctagag catcagagga tataattcag ggggcatcta
    39421 aagctctaca aactggtgta aaatcggtat cagacagttt gaagatagct aaacaatcag
    39481 cagtttttgc aaatgttggt gatttagacc aagaaactgc tgataagtac ttaactagtg
    39541 ttatgtccgc ttatggtgga atgacaaaag cattaaaacc aattaaagat actagagttc
    39601 aaatcaaagg aatgagtaaa gattatgata atcttacaaa atttcttgat ttatcgaact
    39661 atgcaggtaa taattttgca ataacaactg gcgatgtagg agctgcatta caaagaagtg
    39721 caagtatgtt atcatcagca ggcgtttcaa tggaagactc tgttgctttg atagttggag
    39781 gaaatgaatc tgtacaaaat gctgaaaaag taggtacagc actcaaaaca attggtataa
    39841 acatgagtgg tatcaaagct tcagcagatt caggaaaaat cagcctcaat aaaacagcaa
    39901 aaactttaca agaaacagca aaaataaatg tgttagataa gcaaactggc caagtaagag
    39961 atatgatgtc aattttagat gagttggcag aaaagtggca tagtgttggc gaagatgcct
    40021 taactaaaaa tcaaaaaagt ggtattgccg aagcgattaa ttttagtcgc cttatatagt
    40081 aatatataat gataaactcc tttaattgct ggaaactccc gttaggcttt tagtaccaaa
    40141 gtgtgaaaaa ctaaaagata gggacaatca gcagccaaaa ctcaatgaga gtaaggttca
    40201 acgactaggt tataaaccgt acactataag ctattgatag tggaaatggg gagcatcctt
    40261 ataggatgaa gatatagtct catctatatg gaaacatata gcagttcata agagaacgag
    40321 ttaagtatag cgaacttaat tgaagatttt gttcaggtaa aaaccacata aatactttca
    40381 tggctgtcat ggataattgg aaccaggtaa aaaaattcca aagtgcatgg ctgggtggag
    40441 aagtgctagg tagtgccagt aaagagaatg agagattcat aaattcagca gaaggtaaga
    40501 ttataaaatt aaaagaaagt cttaaacaat tagttacaga tactatatct acagatatgt
    40561 ttaagactag tttagatgga ttatctggta ttacaggaat tttaaatggt ataactaaag
    40621 cggctgacaa aatgcatgtt tcgcttcctg tagccctagc aggtttatcg tctttattta
    40681 tgacaattaa agcattaggt actggtaaac caatcacgaa tttaggagga agtatattta
    40741 gtgcctttca aaacaataga aaatctgtta aatttaaaga ctttgaaaga gctacaaaat
    40801 ttactaatca atatacaagt gctataaata aaaatactaa tgcttcaaaa aacaatgcta
    40861 aatcaaatca agttgttaat agaactataa atagtcaaaa tggcattgta ggaaaatata
    40921 catcaacaac caacagttta aaaaaggcaa cagctaaaga aataaaaata aatgaagaca
    40981 gaacaaagtc atatgaaaaa tataatcaaa aattaaaagt tgtttctaaa agtagaaaag
    41041 atacaataag tgatgggctt ggtaatattg gtaaggcatt tgcagggagt agcattgtaa
    41101 attttggtaa aggtatagca actacagttg gaaattcttt agttttaact gctgcatttg
    41161 caggtgtaag tctattagca caagctatgg agaattatgc aaatagagaa gaaaatgctt
    41221 atcaagctag aaagaaaaat attcaagctt caaaacaaca aattaattca tatgaaagcc
    41281 aaaaagttca attacaagca cttgcagaag aatatgataa tttatctaaa aaagaaaata
    41341 agtctaaaga agataataat agattaaatg aactaaaaca acagattgca aaaataaaac
    41401 cagatgcagt tataggaaca gatgaaaatg gtatacctat tttaaaaggt caggttactg
    41461 atttaattgc tgaaatagat agagctatta atgcaaaaga aagattgatg tcctatgata
    41521 aacatgataa tgcgaaaact gctgctaaaa aattaaattc tccaagtaaa gatgtaaata
    41581 aaactttaga agaaagaatg agagaaagtg aaactggtaa gcttgtaaaa atagaagaag
    41641 attatacagc agaagttgaa aagaataaaa aaagacaaga aaaagcaatc gagaaatata
    41701 ataattcaac tggtagagca agagataaag ccagaaatga attaatgtca gctaaagcag
    41761 aagaagaaaa aatatatttc aaatatgatg aaatgtatag aaatcaagta gataaaattc
    41821 aagggtattc aaaagaaata ggagatggaa tattttctaa cattaagaat aaaactcttt
    41881 atagtggatt agaaggcaat gataagtcta gttttgctgg acttgaaagt ttatttgatt
    41941 ttagtgaagt tacaccagat actctagtag acacagaaca ggcagtcaac aaattgctta
    42001 ctgctgttag aagtgggaaa gttgatgtag gagatttatc taaaactctt aaggatgcaa
    42061 atgaagaatt tgctagaaca caagatatag aaaaatataa tcaaacaata gataaaacag
    42121 ctaagagtat agctaaagca actaacacag atgctaatat atgggaaaat ttatttgggc
    42181 aagtaaatcc aaatggaatt aaagatatga catctataaa tacattatta gctaagtttg
    42241 gtaaaacaaa actagattta gcaaatggtg ataaacttgc aatgcaactc caaaatcaat
    42301 ttgatagtat tcagaatata ttagatacaa caacaataac tggagatgta aaagttgatg
    42361 caaatatatt aactgacatt aagaatacta aagaagttcc tagtcaagtt aaaggcatga
    42421 tagatgcttt attaggaaca ggggcaagtt ctactgatgt attaaaattc actatggatg
    42481 tattaatgga attacagact ggtgacccag atataactaa acttcaaaat gatttagata
    42541 ataaatttgg aaaaggtaag tttactataa ctccagaaat acttttaagt aatgattcag
    42601 gtcaagctaa tgcagaacaa ataataagta gtttaaagca aagatatgag gagcttccag
    42661 aagaagtaat cacagtcata aaagctaatc caactgcaac attagaagaa gctgattcag
    42721 taaaaagaat ttatgataaa tttccaaaag aagtaaaaac tatcataaaa gaagaaggag
    42781 cagacgaagg tggtagaaaa atattagatt taacatctaa gtatgccgaa gtaccttctg
    42841 aactcaaaac taaattagaa gctgatggag ttggactaga aaaagctgtt gaagtatcag
    42901 agatatataa gaaagtacct gcggaactta aaacatattt tatagcagaa gcgggagagg
    42961 ctttgtataa ttctgttaat ttaaaagatg ctttagaaca tatacctgat gagaaaatta
    43021 cagaaatata tttgaagcaa aatgatggta aattaggggt tcaggattta ataacatcat
    43081 tggataaaat tcctttagat aaggatgtaa gaataaatat atacaaagcc ttatctgatg
    43141 gagatataga tgctttaggc aaagctattg aaagtttacc accagataaa cgagtagaaa
    43201 taattgccga aatagaaaaa gctaaagatg atattgagac tattagttct tctgaaatag
    43261 caaataaaat ttttactata gaggttaaag acttagcatc aggtgctatt gaatggattc
    43321 aaaaaaaatt aaaagaaata aatggtgata aagataagaa agaccctgtt aaagaagcta
    43381 aagaatctac taaaaaggtt ttaaaagaca aaccgaatcc taaaagtgtt gcattttcaa
    43441 gggataaaga agcagaaaag cttgtagatg atatattaaa tacacctcca acaaaacaaa
    43501 ttgaacttaa gttaaagaaa aatgaagagc ttagaaatac tttaaattca tttaaacaac
    43561 ttgaaggaag aaatgatgta aaattaaaat tagaatcatc aggtattgag actgaacaag
    43621 ttaaaatttt tgctgatata gtaaaaaatc ttccaacaaa ttcaactttt actaataaat
    43681 ttataactga taatgttgat agtttaaaaa atctaaagga ctatgaagcc gtacaaaaat
    43741 ggcttatgga taatccaact atagctatgg attatagcat aaatttaaat ggtttagatg
    43801 attttgataa aatgaaatct atatatgatg gattagaaga caaaaaagat aaaaaagtat
    43861 ttgccgaagt tataattaaa aatccagaaa aggctgaagc atttcaaaaa ctatatgata
    43921 atgttccaga agaaacaaaa agtaaggttg ttagctttgc tgtagaaaat gcagatgagc
    43981 ttgagagagc tacaaaacta tatgaatcaa caccagatga acaaaaaaat caagttctta
    44041 attttatgtt aaataatgaa gataagttat atttaataga acaactttat aatgatttcc
    44101 ctgaaagcaa agacattata gctaatctta tagtaaataa tccagatgca ttagatgaag
    44161 ttgagaattt aaatgcttta gacctagaca aagacgtaaa aataaatata ataaaatcac
    44221 taactaatgg agatattaat tcattaattg ctgaaataga aaaattacct cctgaaaaac
    44281 aagttgaggt aattgcagct attgaaggag ctattgaggg catagatagt gttgataaaa
    44341 aaacaataaa agacaagtgg gctaaactta aagcgaataa ttctgctgct ttagaaacaa
    44401 ttcgtcaaac tgaaaataaa aaactaagtg ataagagttt tacaataaca gcacatcttc
    44461 gtacagtagg gactctagga ggaattgctt ctcaatttgc aaataatata ttaggtggat
    44521 taaagagtaa tagaaaaagt gtgcaatcca taaattctat agaaactaca caagcaattc
    44581 cagctaatct atcagcccaa cctagaacgt cagaaccagt accaataagt gacgaaactc
    44641 ctgtaacaaa accatctcta ttctcaagag cagtatcaag agccacatct ccagtcaaaa
    44701 ctcttacgga gaaattctct cgtataacta aaactcctaa aatagcatta gatacaaaat
    44761 ctattgatgc agcagtaaaa tatagcattg agttactaaa agaattagaa aatgcgatat
    44821 ctaaagttac agatagaata tctcttttag ataagaaaat gaaatatgca agtggaaaag
    44881 ataaacttaa atatcttgaa gaacaaaacg ctttatataa agaagaacta acattgttag
    44941 accaaaaaga taaggcactt aatactcaaa agaatagact aaaagataga cttaaaaaag
    45001 attataagta cactttttca gatgatgata acttaactag ttatgaagaa aaattaatat
    45061 cattagaaaa agaattagag aagttagata agcaatcaga gagcgacaaa ggtagtaaaa
    45121 agagtgaaga acgtaagaag aaaatagaag aagagaaaaa ggttgttgaa gaatatttaa
    45181 agattgcttt tactgagata ccgaaagttg cagatgctca acaagaagta acaaattctc
    45241 ttatagaatc aactagagca gcagaggaat ttaaaaaaga attaaaagag attgctagag
    45301 aagctgcttt aacttcagct caaaaacatg ttactgaaat acaaaacgaa atagacttaa
    45361 tagacattct tatgaaaaat gctgaaggtg atgaaaagtt agacttaatt gaaaagaaga
    45421 aagagttatt ggccaaacaa gctaaggaaa ttaaagatgt tattaaagtc tatgaagata
    45481 ctgcaaaaga gttaagagaa gggttatcaa aagaaggatt tgaattttct gctgatggta
    45541 aaaacataac aaattatgag caacaattag cgtttctaaa aaataacaaa gatgcagata
    45601 aatataaaga aatagaagaa aatgctaaaa aatatttaga tttacttctt aaagatttac
    45661 ctgatgctaa taatcaaatt aagcaaaata aagaagaaat agaaaattta aataaagaga
    45721 ttcaagatgc ttataaagaa cagcttaaag aagctcaaag tctacaagaa aaaattagag
    45781 acatgtataa aaaagagctt gaagagagac ttaaagaaat agataaggaa actaaagcta
    45841 aaattgattc tcttaaaaag caacaagatg cttacaatga ttcaagaaaa gaagccaaat
    45901 ataaagatga ttatgaagaa caacaagatg taataagtga tttagagaaa caaatagcaa
    45961 tagctgaaag agatagttct ttaagtggtc agaagaaact taaagattta caaaaacaat
    46021 taaaagaaga acagaagaaa ttacaggatt tagtacaaga ccatgtggac gaccaagtca
    46081 atgatatgta tgataaggag tctgacagac tacaagaaga agcagataaa ttaaaagaag
    46141 agttagagaa aaaatattca gatgaaaatt tagttgattt aattaatgaa gcaatttcaa
    46201 gcggtaaatt tgtaggtctt gatggagagg ttaagaaact tcaagatgct attattgaat
    46261 acataaataa atatgaagat ggtatgttgg caatgggttc tgtcactaag caggaatggc
    46321 tcgatagatt aaaagaaggt aaagagacat tagaagatat taatgacata ttggatgaat
    46381 tagatttaag taaatttgct atgcctaatt ataatcctcc ttcaaattct cgttcaagaa
    46441 gtacatctcc aatatcagca gttaattata attcaccatt tgttgttgta caagggaatg
    46501 taactaaaga tgtaatgcct gaatttgaaa agaagatgag gaaaatgatt gaagaaaata
    46561 acaaaaaaat tatttctaat actaggtctt aatatatgta attggtcttt atttattatt
    46621 cactcattta cttacgtttt ataatttata gtcagatttt ttttgaaaat atatatttat
    46681 ctaataaaaa atattgttta taaaaaacgt atttatgata taatagtaat ataaatgata
    46741 taaatttata tcataaagac ataaaaaaag agactacaac tatttgtgct agggtgtctc
    46801 ttcataaata agcgaagtgt taattagagg aagaatcacc tgaattgccg ttcgggtgat
    46861 tcttaatttt ttgcaacgtt ttcttaaact catctatttc tttaaagagt ctatttaaat
    46921 tctgaaccaa cttaatcaaa ctaataatta tagttgtaag aaatgttatt atcgttagca
    46981 aacaaattag atatttcata atatcatatc acctcccctc ttgatttgac atgggaaggc
    47041 ttatgtatat gaagattcac cctaatagtt ttttttatgt agtctctaaa aatattatac
    47101 cataaattac caatatgtta aatttaatat tatattgtct acaattgtaa aagaatattg
    47161 tgttctaaga agttattcta ttaataatat agctttttta tttttcaatg aagataatga
    47221 caacattgac tattttgata atattggagt ataatagtaa tataaatgat ataaatttat
    47281 atcataggga cataaaaaag agactacaaa ctatttgctg tagggtgtag ttcttaaagt
    47341 taacaataaa aagaatagat ttatgtatta ctttcagaat cactagaccc gccagtctgg
    47401 tgattcttta gtttcctaat agatgctttc aagtttaaga ttgcatctat tagctttgtc
    47461 aaatgattga ttattcttgt aatcataatt atcattatta aaaacaaaac taagtactcc
    47521 atagtctcac cccctttctt actggggatt ttaactaaag agccatcacc ctaatagtct
    47581 aaactgtagt ctctaaaaat attataccat aattttccat aatgttaaat ttaatattat
    47641 attgacgaca gcagtagaat aaagatataa tacaggtaac agaaaattaa aatattttta
    47701 cataaagaat ttctacttat ctaggccacc ttaaacaagt ggctattttt atactctggt
    47761 atatatttaa ggaggtgtta caaatgataa ataatataga agattgtaag cttcttattg
    47821 agacagaaat aagaatgaaa ggtatagatg aaaaagaact aatatctcaa agcgaaattc
    47881 tgaaagacct aaatattaat gaaaaagacc ttgaagatat tgaagtgaat tttgaataat
    47941 gaaatgattt ataatatacc tatttaaaac atgtattaat tgatatcagt aatagtgatt
    48001 ttttatttat taactgtaaa cgaatctgtt ttacaaaaaa cgtatttatg atataataaa
    48061 agtaaggaat ttaatctact taatacaaag agtgattgtt ccttaaagtt aattaaaaaa
    48121 tcaatttatc ttttgaaacc actcttattg gcgtttgagt ggttttttgc tttatcatat
    48181 atgtaacaag ctattaaact tgttgcaata tttagattaa taaaatattt tagctcatct
    48241 taattgatgg gcttttctaa tacaaagaag gtgataaaat atgcatttta atgtgaatct
    48301 taaacagata aaatctgatt acacattaac cattcacaag atgaacaaat catttttagg
    48361 tcaaatccca atcaactttt taaattctat aaagcgtgag cttggtggag tagatgaaat
    48421 acaactgacc attccaaaat atattacaga tagattttta tttaataaaa taataaatcc
    48481 catttttgaa gaagtaaaag aagaacgtct tatttgtctt aataataaag aatattttgt
    48541 agttaagaat gttgtgacta cagacgataa attaaaagta gtaacagcta aatctaaaga
    48601 agttaaacta ggaaaaattg atgtgaatat tgaggactat ggattacaga tgtttaccaa
    48661 agatgaagaa gcatctatta tatctcttaa tgattattta aaacaagaga caggctggaa
    48721 acttggttat gtggatgatt caattgctta tgaaactgat agtgaaggta ataagagaga
    48781 aaaagtaaga tggcaagaga gtattaattc taattggtta gattatttca ataatgaact
    48841 aaaagaacaa tttgagtgta tagctgattt tgacacttat aataatttag ttaatttgta
    48901 tcatatagac agttttggag ataacattca gttatattta tctcatgata actacataaa
    48961 atcacttgaa agaactacaa atagtgatga tatagtaact agactgaagc ttgaaggtag
    49021 tgaagatatg aatgtaatag gagctacagt tacaggatat gattacattg agaattattc
    49081 ttatttctta gacaataaag aaatgagtga agaacttagt agagctataa agaaatatca
    49141 agaaatgaat gaaataagag agccaatttg gagagaatta atagatacaa agcttaagaa
    49201 acaaagagaa cgtgatagta aaagcaacga atggcaaatg gtaatagaaa tgataagtaa
    49261 gaaaaaagat ataaaaaaga catatgataa tccacaacat aaagatgaag taaattcagc
    49321 taaaatagca gtagagataa gtgaactaga agataaaaaa gtcatattgg atgttcaaat
    49381 aaaacattta gaagaagaaa tagctaagtt aaatgaaagt ataaaagata taaatattct
    49441 ttgtaagagg gagacttcaa ctgatgagga tggatatcta atatttaatg aggttctatt
    49501 agatgaatta aatgagttcc tctactatga cacttataca aatgatgctt ttttaaaagt
    49561 tgaagattta atagcagaag gtaaaaggca attaagtttg aaatgtattc caacacgaga
    49621 atggactctg gatgttataa acttcttaga tagaattata gatattaatt ttagacagca
    49681 ttggaagggt gatttaagtc tgggagatat tatagtgtta catagcaaag aatctaaaga
    49741 agaagaatta gtatatttta catcttttac ccagaattta aaaaatggga aattggatac
    49801 tttagaatta actttaagta ataaaaaaat aaaagaagat gacaaaagaa ctatagctga
    49861 ttatttgact aaagctgaac atgcaacaag aacattgaac tctaaaagac atttatttat
    49921 tcagcaacag aagaaaagaa ttaacctacc agatgaatat attcctaaga aaaatataca
    49981 aaaggagctg atgtaaattg atattggata attcaccagc agattcatgg attagaatta
    50041 ctggagtaat tgttacttat aataacacac tttatcaggt agtagataaa gaaacaaata
    50101 aaaaatatat atactgggat gctgataatc cagggacatt gaaagtttct aatgtaagat
    50161 taccagaagg aaatacacaa tttttagtag ttgtaaatga taatggaaag catacagaag
    50221 ttcctataaa ctcatcaata ttcaatatat cttttgatgg taattcaaga aaaaacactg
    50281 aagaacaaat ctggggattg tatgaaactg atgaagaaca caatgaaaag tttgtagtta
    50341 ttgaaaaaga catagatgga atacatcaaa cagttttaga agtacaagaa gatgcatctc
    50401 atataaaaga aaatatgtct cttatagacc agagagctga aaatgtaaat atattagtaa
    50461 aagaagtaac taaaaatttt ggtggttcac aagaaaatat aatattaaga gaaaacataa
    50521 ataaagctat aattaagtta aatacagact taggaacatt tagctctaat atgtctaatt
    50581 attttaacga taatgagatt acagatgaag aaaaagaaaa gattgatatt gaacttaatt
    50641 tattagatac agacaaaaca agtttatata tagagctaca aaaacttatt gatagaacta
    50701 ctggagtaga cttagtagca ataaatactt caaaaacagc attgaatgca gcaagtacca
    50761 atctaaactc tataatcaat tcagttattt cagatggtat aattacacct tcagacagaa
    50821 ttttagctat aaatgcaaat gctcaatata acttaaagat aaatgaactt aaaaatacag
    50881 tcgataaaat ttacataaca ggtatgggtg gaagtatatc agaggaattt tcacagataa
    50941 atgctacagc taaagaaata aaattagaag tagcaaaagt tgatggtgca actaaaacaa
    51001 atgctgctga aattaaatta actaaagatg atataaccat gatggttaca aggaataact
    51061 ctggctcaat tgtaggaatt aaacctgata aaattgaatt tggatttaat gatatatcaa
    51121 attatgtaga gataagcagg agtggtctaa cagtaaatca aggagctata gcatgtgata
    51181 tattaactac tccatctgga catgaaccaa taatcagatt atttggaagt agcaggtctg
    51241 gatttgcaat agatgcaaga caatcagatg gttctagtca agcctcagca ataagattaa
    51301 aatatgatag caataattat ttttgggtag gatatgatac tgctgagatt tatgttgatg
    51361 gagaagaaca ctttattgtt gaaagagatg atacttttgt aagatgtggt ggagctactt
    51421 ttacatttac aaatggagat agtttaggtg taggatattc attttatcca gagcgttctg
    51481 taactgattt aggttgtgag cgttataaat ggagatattt atatgctcgt tctactctga
    51541 gtgaatctga taaaaagttt aaagagaata tagtatacat aaaagatatt aagaatagaa
    51601 ctcgttcaag tatcactcca acaccatttt tagattttat aagagatgat tttaaaccag
    51661 caacatttga ttatatagca gaaaaagata ggacaattgc agatagtcaa atagggttta
    51721 tagctaatga ttttaaagat agttatgttg gaaaaacatt tctatatgac tatggagaag
    51781 aaaatggatt aatgtttagc ccatctggtt atacaactgt tgtagcaact gcacttcaag
    51841 aagaaataca aaaaagagaa gaattagaga tgataattaa tgaattaaac gagaaaatta
    51901 ataatttagg aggatattaa tatggaagta aatttacaaa aagcatatac agtagcattt
    51961 gaagagataa aaagtttata taatgaacta atactttata aagcactcaa catgcaacaa
    52021 caagaagaaa ttgagaactt gaaaaaagaa ctagaagaac aaaataaaga acaataggat
    52081 gtgatacttt gaaaaattat gaaataaaga atcataccat agaggtcgac tttagtaatt
    52141 atagaataaa caaaagattg ctagattatt ttatttataa tgaaaatgat gttaaaacag
    52201 cttatattga agctatattg aaaaataaag atgaaataat agatttatct gaatatgata
    52261 gagttttagt tagcattaca aaaagtgatg gacaaaaggt taatggtgaa tgtgaagttg
    52321 tagatgcaga aaatggtatt gtagagatag aacttagccg acaagcactt gcttctgttg
    52381 gtataaatac atttcaacta tcccttgtga aagatggaac tttactcaat actacaaatc
    52441 tttattatag agttgaagag ggtatgatta atgatgatga tatcacctca actgatgaat
    52501 atggagtatt attggtaata atagcccaag cagaagagat tataaaaaat aataaagagt
    52561 tgactaaaag agttgaacag cttgaaataa ctatacttgg aaatgaagaa gttagggata
    52621 aagcagagca gattaggatt tataatgaag atataagaaa catacaagaa gaagaaagag
    52681 agtttaatga gttaacacgt caaaatcaag aggcaaatcg tgaagaatcc attcagaata
    52741 tgcaaattca agttgatgat aaacttacag attgccaact ccaattagat gaaatgatag
    52801 atgctaagtc taaagaaata gataatatag tcgatgataa aatgcttgat gttcaagttc
    52861 agacagacaa taagtttcaa gacttagaca ctagagctaa taatactttt gatttgtatg
    52921 ataagacatt tgaagataaa cttacagata accaagagca aattgattac aaacttgatg
    52981 aagtaaatca agctatatct aatgtagaag attgtattga tgaaagtact acaaaattag
    53041 atacaaagat aaaagaagta gatgacaaaa tagttgaagt aaatactgct aaaactgata
    53101 tgacaacaac tgttagtaat aaaataactg aatttgaaaa taggtttgaa gaattagaaa
    53161 gtcttgatgc tagaggcgaa ttaatacaag ctagagaaag tgttgatgga actgtaaagg
    53221 atactttaaa ggatagatta acatatgact ttgagaaagt taacgagaaa atagcagaaa
    53281 tgacttctgc tgcaactaat gtagctttta gtaaatctta tgttgaatcg gattgggttg
    53341 ctgatggaga atattttaaa cttatagtta atcataattt agttacagaa aatatatttg
    53401 tagcaatatt agatgaagca actaagaaaa gtatgactaa ttcttatact acagtagatt
    53461 ctaatacgat agaaatattt aatgaaagta acatagatgt aaaagtaact gttgtaaatg
    53521 gtaatacaaa taaagaagtt atacaagcta caataaatga taatataaca acactagata
    53581 gtacttactc tagtattaaa attgatgcaa aatttgatga gagtctaact aagataaatg
    53641 aaaataaaag taatatagct actaacttag agaaaataaa tctaatacaa agtaaagttg
    53701 gttcgagtga attaagtaca gtatctaaaa atatatcaga tgcggtaaat gaattagatg
    53761 ctagtgttaa agttcttgaa tctggtggaa atacaaacga gcaattgaaa atattaaaag
    53821 aaaattataa taaaatgtct gtggaaatac ttaaaattct atttgaatta gaattagata
    53881 aggaagctac agcagatgaa gctgggtatt ggtatgatac cctatatgat tctaaaaaca
    53941 taagtaattt agatggttta aagcttaata aatttaagca acaattagag ttactttcaa
    54001 atagtggaac tgtattattt aaaaatgtaa cagttccttt tttatgcaac aaaataagat
    54061 atattcatga tttaggaagc aactatagtg aaactaaaac agaagaatct tatcaaaaag
    54121 gtgatactga aataaaaata aacaagtatt cctacgaagt gagatagggg gaattatatg
    54181 aaaaagaata aactattaca aagaggttct ttatttggaa acaaagaatt taaaatagaa
    54241 gagtttaata aaaattttga tacttttaat gttaaagata tatttacaga tatggattct
    54301 ggttctgtgt ttataattaa ggatgattta aagttatatg cagcaggata taatgttttt
    54361 ggcaatttag gacttggaaa taaagtatca cctataagat atttttcaaa aatagatatt
    54421 gacaatgtta agtatgtttc atcttctgga actcattctc ttttacttaa aaataacgga
    54481 gatgtgtatt ctactggctt aaacacagat ggggagttag gtttgggaga taatattgat
    54541 agaaatactt ttactaagat aaatataagt aatgtaaaac aaattgcttg tgggaatggc
    54601 tattctatgt tagttacaaa tgacaacgag ctttatgtat gtggtaataa taaatttgga
    54661 gggataggct taggaaatac atctactgta aatacattta ctaaaattga taatttgagt
    54721 gttaaagaaa tatttgcagg agtcagttgt tcatatatat taactttaaa taatgaagtc
    54781 tattcaacag gtacaaattc ctatgggcag ttaggactta atgacactac taatagaaat
    54841 atttttacta aagtaaattt agataatgta ataaagattt ctactgctca aggtgctgta
    54901 aattttcttt tagaaaatgg ggatgtctat ggttgtggag ataattcaaa tgggcaagtt
    54961 ggtgttggtg gaggagctac agtaacagtc ttaacaaaac tatctatatc taatgtaaaa
    55021 aatatagaaa gtggtagata tcataaattt tatatagcta ataataatga tatatatgta
    55081 actggagcca acccagatgg gcgtttagga gataaaataa attgtgatag atcaacatat
    55141 actcctagaa aatcttctat tagtattaaa aatataagta aaattaaatg tgctcatgct
    55201 ataagcttta tattaactag caacaatgaa ttgtatatga caggagataa cagttatagt
    55261 ttttcttatt taaaaagata tgataaattt atatatggat ttgaaaaatt tgaggacttt
    55321 gatattgaag aatgtaatca tattgagtct attgataata gattattaat aaataaaaat
    55381 gatgaaagag ttttagatat agggcttgaa aataattttg tgaatatctc tttagtgaaa
    55441 gcaaatgaca cctctacatt tattgttgtg aatggtaatg aactatacgc agcaggatac
    55501 aacgcttatg gaggtttagg tttaggtcat aatcgtcatt gttatgagtt tactagagtg
    55561 ccattggaat tagaagaagg agttactata aaagatatat atttaaatct aggctcatat
    55621 ttcacgttta tattactaag tgataataca ttatattcaa caggatataa tgtatttgga
    55681 gtgttaggat taggggataa tattaataga aatactttta ctaaagtaaa tatttcttct
    55741 gttgacaaaa tttctgtagg agataaccat gcgttattaa aaacaactac taatgaatta
    55801 ttttcaactg gtttaaacaa ttatgggcag ttaggactta atgacactac taatagaaat
    55861 atttttacta aagtaagtac tgtaacttat gtaaatgaaa ttattgcata ttcaaattct
    55921 tcatattgca tagacaatag cagaatatct tatgcagcag gatataatgt ttttggcgaa
    55981 ttaagtctta atgatacctc aagtaggagc gtttttgtta agatgagata ttcaagatat
    56041 aaaacagata caacagtatc agatgtatct aacattgctg aaattattcc ttttaataat
    56101 ggatgtttaa ttagaactac gtctaataac tactatgttt ctgggagaca taattattat
    56161 agcacaagta ttaaaaattc tgtaactgat gattattatt caaggtttgg agagtttgta
    56221 ggaagtaaaa actcaaatgc aaatgaagtc gcagttttaa tagatgaacc taaattatca
    56281 aaagttctgg tttctaacta taatttatgt ttattattag atgctagtga tggaggatat
    56341 aaagaaataa aattcacagg aagaccttcg acttttggag cttcatccca agacaagaat
    56401 ttaggtttaa gaagtcttat atcgtcttct atgccttatt ataatcatta tcttggaaca
    56461 gacaatcttt tatatacaac aagtaaaaca aggatggagt ctaaatttga aataagaggt
    56521 agaaatgcag gtgggcaaag aggagacagt agttattatt ctaatagtca aaaatacaca
    56581 ttatcaaatg ctttttttgc atataattca caagtaatat atgctagtgg attaaggata
    56641 gtaattaaag attattttgg gaacatatat tgttctggag taaattctca tggctgtttt
    56701 ggcataaaaa aaccttatga tgttagacat gatggttttg taaatatatc aaaacaattt
    56761 tctaaaaaat atatcatatc aaatataaaa gaaattaaaa gtgatagaca agctttatat
    56821 atattattaa ataatagcga aggatatgtc tgtggtaata atgctggtaa caagttaggg
    56881 acaattaaag ctcggtctga atttataaaa gttcctatag ataatataaa agaatttatt
    56941 ccatcgaatg attgtttatt tattcttaca aataataatc aagtctattc aattggaaca
    57001 aatgacttag ggcaattagg tttaggagat aatattgata ggggtacttt tactaaggtg
    57061 cctattgata atgtaaaaaa agtaataaca tgttacaaat cttcttacat tctaacctat
    57121 gacaatgaat tattttcaac tggttcaaat ggctatggtc aattaggatt aggggataat
    57181 attaatagaa atacttttac taaagtaaat atttctggaa caataaagga tatattttgt
    57241 ggaaggtatg tttttattat attattggag aatgataata taatttcttt aatgggttgt
    57301 ggaaatagaa attacataag ttcaaataca aattcaaaca gtttaactaa aataaatatt
    57361 gaaggattaa cagacccatc aactatatca aatttaatgt tggaagaagg caaaactata
    57421 attacaatga aaaatagaat atttgtcaca ggatataact cattaggagg tttaggtgtt
    57481 ggtacttcat ataataattc atcaggatat attgaaggat ataaggattt agcgtttaat
    57541 agtaatataa aagaggttgc tctaggagaa tacactattt ttgttttact tgaaaatgga
    57601 gatttatatt ctggaggaaa tgctgatgat ggtataggtt ttaatgaatc tggtactgta
    57661 gtattaaaaa aattaacaag taatataaag cacatatatg caaatggtaa aaatgcttat
    57721 tatatagata atgataatgc cttatatgtg tttggtagta gacaattggg tcaaatcaat
    57781 aattattctg atgttgtaca aaaaacacct gttagaaatt gccttaatgt aaaacaggta
    57841 tatacacagc ataatggtct tatagttcaa tatactaata ataaattaga aggaaaaggt
    57901 aatgctgtag ttggtcaagt aggtattttt aatggacatg aagaaaatca gattgcaaac
    57961 acttctggtg ataatcctag tgatttaagt ttttctaatg aaatgtctat tataagaaat
    58021 aattatggac tattagtaaa taagaagata tttttacata atgatgatag tattaattat
    58081 agaataccat ttggcacaaa tgctggaaat tactcaactt cattgccttt tgataatata
    58141 aaaaagatgt ctttatcatc aactcattca attttgatat tagaaaatgg ggatgtctat
    58201 ggttgtggtt caaatcaatt tggggaactt gctataaata aatctgagct aacaacaact
    58261 tctgaattta taaaaattaa cttaacaaat ataatagata ttgcttgtgg tgataatttt
    58321 acttacttct taaaaaatga tggctcattg ttttctattg gtaaaaactc agaatatcaa
    58381 cttggaatag ggcataatga tgaggttaat gagttacaag aagtattaac tatttctaat
    58441 ataagggaga tatatgctta tagtaattat atattggcta taactacaga aggtcaatta
    58501 tacgtacaag gatataatat aaatgggatt ttaggactga atgagaatac aaaaaataca
    58561 attattaaat tatttactaa agtattagaa aatgtaaaat atataaaatc ttatgatgat
    58621 aaacatatat ttgttataaa aaatgataag actttgtatg ttacagggat aaataaggaa
    58681 acatataaaa ttaaagatgt agagattggt tctatgttat atactttctc acaaatatat
    58741 attccaagag atgttaatga tatagtggat gctttaataa aagatgaaac attatatata
    58801 atctcaaaag tagattcaga gaaaacttgt ttggaaataa agaataagtc atactcatct
    58861 attgaaatag atttgcaaaa tccaaataat gaattgacaa aaatagagat gtttataaat
    58921 aatattagtt caagtataat agaagactta tctacaggaa cagtcacgtt tgagattaat
    58981 ccagagaatt tagttcttgg agaaaataag attgtattta aagcacattc tgacattgga
    59041 aataatttat atataaatgt ttatatttat aaaaaagaaa caggagcaac tatagtaaaa
    59101 gattcaactg tattaatcga tgggaatact tataatgtat ctagtatagt tgataattca
    59161 caagatgtag tacttacact caataaaggt ctgttagaag atttaaattc taacaatcca
    59221 atttatcatt tagtaaataa attaaaagta caattaaaga taaatgaatc agatacattt
    59281 aaagatatgg ttaaagtaga gacaagaaaa actgagaatg gatataagga gatatatgag
    59341 ttaaaagata tgaatataca atctgcacaa cctaaagtaa tagtagaaga aggaaacact
    59401 aatacaacta taaaaaaacc atctatgtta ttcaatttag atgttgaaac actttaagag
    59461 gtgattaaat gataaaaata gataaagaaa aaattgagaa gtcaagacaa agaaaagaat
    59521 tagatgattt agattcaagt caaaaaacgc aagataatga cataacagat ttaatgttag
    59581 cgactgctga aatatgcgaa atggtattaa gtagtcaaca gacatctact atgtcattaa
    59641 aaaatataaa attaaatgaa ggaggaagta gtatggcagc aatttatgta ggattgatag
    59701 agagaggttt aaaaacaatt gaccaagtac ctgttaaata tcgagaagaa gtaagaaaaa
    59761 tgtgtgaagc actagagatt tcattatcat agaactgtaa gagttctttt ttttatgttc
    59821 aaaatagaga gttactaatg tagctctcta aataaaaaag aaggtgataa attgttaaat
    59881 gaagatgtag taaaaaaatt aaaaaatgta cctaataaca ctaatacaga aatagaaaaa
    59941 gttaatacaa atatagaaac tgctaaaaca gagttgaata ctaaaattga ccaacttatc
    60001 gcaggtggtt caaatgtggc atctactcaa acaataacaa ttgatgattg ggtggatgat
    60061 acagaaaatg gatttaaagc aactgtaaca catggtttgt taacacagag aatagttgta
    60121 aatattatag atgctactac aaaagaaaat gtagttacaa attttaaaat tatagatgat
    60181 aattctatag aa
//
LOCUS       DAENCV010000007        58935 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAENCV010000007 DAENCV010000000
VERSION     DAENCV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.007"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            248..1324
                     /locus_tag="KQD82_000388"
     CDS             248..1324
                     /locus_tag="KQD82_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4378892.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1441..2283)
                     /locus_tag="KQD82_000389"
     CDS             complement(1441..2283)
                     /locus_tag="KQD82_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG4378893.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2467..3039
                     /locus_tag="KQD82_000390"
     CDS             2467..3039
                     /locus_tag="KQD82_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4378894.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSKNEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3202..3474)
                     /locus_tag="KQD82_000391"
     CDS             complement(3202..3474)
                     /locus_tag="KQD82_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378895.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3601..4473)
                     /locus_tag="KQD82_000392"
     CDS             complement(3601..4473)
                     /locus_tag="KQD82_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4378896.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4769..7876)
                     /locus_tag="KQD82_000393"
     CDS             complement(4769..7876)
                     /locus_tag="KQD82_000393"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG4378897.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7969..8201)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8376..8903)
                     /locus_tag="KQD82_000394"
     CDS             complement(8376..8903)
                     /locus_tag="KQD82_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG4378898.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8938..9723)
                     /locus_tag="KQD82_000395"
     CDS             complement(8938..9723)
                     /locus_tag="KQD82_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG4378899.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9733..9990)
                     /locus_tag="KQD82_000396"
     CDS             complement(9733..9990)
                     /locus_tag="KQD82_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG4378900.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(10009..10467)
                     /locus_tag="KQD82_000397"
     CDS             complement(10009..10467)
                     /locus_tag="KQD82_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG4378901.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     TKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10507..11211)
                     /locus_tag="KQD82_000398"
     CDS             complement(10507..11211)
                     /locus_tag="KQD82_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG4378902.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11221..11856)
                     /locus_tag="KQD82_000399"
     CDS             complement(11221..11856)
                     /locus_tag="KQD82_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378903.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12237..13277
                     /locus_tag="KQD82_000400"
     CDS             12237..13277
                     /locus_tag="KQD82_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4378904.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13740..15095)
                     /locus_tag="KQD82_000401"
     CDS             complement(13740..15095)
                     /locus_tag="KQD82_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG4378905.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDSVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15121..15390)
                     /locus_tag="KQD82_000402"
     CDS             complement(15121..15390)
                     /locus_tag="KQD82_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG4378906.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15680..17650)
                     /locus_tag="KQD82_000403"
     CDS             complement(15680..17650)
                     /locus_tag="KQD82_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378907.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENNNNENDNSD
                     EKQPNTPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLS
                     SKSVFGTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF
                     LFCILTPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17833..19308)
                     /gene="spoIVA"
                     /locus_tag="KQD82_000404"
     CDS             complement(17833..19308)
                     /gene="spoIVA"
                     /locus_tag="KQD82_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG4378908.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19480..20775)
                     /locus_tag="KQD82_000405"
     CDS             complement(19480..20775)
                     /locus_tag="KQD82_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4378909.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20959..22368)
                     /locus_tag="KQD82_000406"
     CDS             complement(20959..22368)
                     /locus_tag="KQD82_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4378910.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22420..23559)
                     /locus_tag="KQD82_000407"
     CDS             complement(22420..23559)
                     /locus_tag="KQD82_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4378911.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23878..24528
                     /locus_tag="KQD82_000408"
     CDS             23878..24528
                     /locus_tag="KQD82_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4378912.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24685..25704)
                     /locus_tag="KQD82_000409"
     CDS             complement(24685..25704)
                     /locus_tag="KQD82_000409"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG4378913.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVSEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25735..26349)
                     /gene="plsY"
                     /locus_tag="KQD82_000410"
     CDS             complement(25735..26349)
                     /gene="plsY"
                     /locus_tag="KQD82_000410"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG4378914.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26384..27703)
                     /gene="der"
                     /locus_tag="KQD82_000411"
     CDS             complement(26384..27703)
                     /gene="der"
                     /locus_tag="KQD82_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG4378915.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27737..29065)
                     /locus_tag="KQD82_000412"
     CDS             complement(27737..29065)
                     /locus_tag="KQD82_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG4378916.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29303..29503)
                     /locus_tag="KQD82_000413"
     CDS             complement(29303..29503)
                     /locus_tag="KQD82_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378917.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29505..30437)
                     /locus_tag="KQD82_000414"
     CDS             complement(29505..30437)
                     /locus_tag="KQD82_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG4378918.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30482..31414)
                     /locus_tag="KQD82_000415"
     CDS             complement(30482..31414)
                     /locus_tag="KQD82_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG4378919.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31469..32758)
                     /locus_tag="KQD82_000416"
     CDS             complement(31469..32758)
                     /locus_tag="KQD82_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4378920.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32787..33479)
                     /locus_tag="KQD82_000417"
     CDS             complement(32787..33479)
                     /locus_tag="KQD82_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4378921.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33494..34255)
                     /gene="pgeF"
                     /locus_tag="KQD82_000418"
     CDS             complement(33494..34255)
                     /gene="pgeF"
                     /locus_tag="KQD82_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG4378922.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34252..34713)
                     /gene="nrdR"
                     /locus_tag="KQD82_000419"
     CDS             complement(34252..34713)
                     /gene="nrdR"
                     /locus_tag="KQD82_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG4378923.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34735..34998)
                     /locus_tag="KQD82_000420"
     CDS             complement(34735..34998)
                     /locus_tag="KQD82_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG4378924.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35102..35875)
                     /gene="sigG"
                     /locus_tag="KQD82_000421"
     CDS             complement(35102..35875)
                     /gene="sigG"
                     /locus_tag="KQD82_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG4378925.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35967..36689)
                     /gene="sigE"
                     /locus_tag="KQD82_000422"
     CDS             complement(35967..36689)
                     /gene="sigE"
                     /locus_tag="KQD82_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG4378926.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36714..37586)
                     /locus_tag="KQD82_000423"
     CDS             complement(36714..37586)
                     /locus_tag="KQD82_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG4378927.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37876..39150)
                     /locus_tag="KQD82_000424"
     CDS             complement(37876..39150)
                     /locus_tag="KQD82_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG4378928.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39434..40591)
                     /gene="ftsZ"
                     /locus_tag="KQD82_000425"
     CDS             complement(39434..40591)
                     /gene="ftsZ"
                     /locus_tag="KQD82_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG4378929.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40781..41134)
                     /locus_tag="KQD82_000426"
     CDS             complement(40781..41134)
                     /locus_tag="KQD82_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG4378930.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41233..41907)
                     /locus_tag="KQD82_000427"
     CDS             complement(41233..41907)
                     /locus_tag="KQD82_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG4378931.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTITGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41912..42610)
                     /locus_tag="KQD82_000428"
     CDS             complement(41912..42610)
                     /locus_tag="KQD82_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG4378932.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42633..43373)
                     /locus_tag="KQD82_000429"
     CDS             complement(42633..43373)
                     /locus_tag="KQD82_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG4378933.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43643..44869)
                     /gene="murG"
                     /locus_tag="KQD82_000430"
     CDS             complement(43643..44869)
                     /gene="murG"
                     /locus_tag="KQD82_000430"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG4378934.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44887..46017)
                     /gene="spoVE"
                     /locus_tag="KQD82_000431"
     CDS             complement(44887..46017)
                     /gene="spoVE"
                     /locus_tag="KQD82_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG4378935.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(46069..47424)
                     /locus_tag="KQD82_000432"
     CDS             complement(46069..47424)
                     /locus_tag="KQD82_000432"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG4378936.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47443..48411)
                     /locus_tag="KQD82_000433"
     CDS             complement(47443..48411)
                     /locus_tag="KQD82_000433"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG4378937.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48452..49825)
                     /locus_tag="KQD82_000434"
     CDS             complement(48452..49825)
                     /locus_tag="KQD82_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG4378938.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49992..51971)
                     /locus_tag="KQD82_000435"
     CDS             complement(49992..51971)
                     /locus_tag="KQD82_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG4378939.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51983..52330)
                     /locus_tag="KQD82_000436"
     CDS             complement(51983..52330)
                     /locus_tag="KQD82_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378940.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52816..53751)
                     /gene="rsmH"
                     /locus_tag="KQD82_000437"
     CDS             complement(52816..53751)
                     /gene="rsmH"
                     /locus_tag="KQD82_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG4378941.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53781..54527)
                     /locus_tag="KQD82_000438"
     CDS             complement(53781..54527)
                     /locus_tag="KQD82_000438"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG4378942.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54921..56207)
                     /locus_tag="KQD82_000439"
     CDS             complement(54921..56207)
                     /locus_tag="KQD82_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG4378943.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56222..57487)
                     /locus_tag="KQD82_000440"
     CDS             complement(56222..57487)
                     /locus_tag="KQD82_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG4378944.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57803..58900
                     /gene="ychF"
                     /locus_tag="KQD82_000441"
     CDS             57803..58900
                     /gene="ychF"
                     /locus_tag="KQD82_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG4378945.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18473 a        10672 c         6342 g        23448 t
ORIGIN      
        1 ttattatttt tcattagtat ataaaaagat aaaatacata actttataga cttaatttat
       61 tacttttata gaattttcac aataaaaagt cattttactt ccgtatttaa tgcaaaagta
      121 gagattaaac ttaatataaa aaatatataa caaatttttt ttatatttct agaagaaaag
      181 tatactcttt tttgtttaat aatgctaaaa ttgatttgta ttgaattttg attaggagga
      241 aattattatg aaagctcaaa gattaaatgc tgtacttgaa caaatgaaaa aagatgatat
      301 ttctcaaatg ttagtaagtg accctacttc tatattttat cttacaggag tactaatcca
      361 tccaggcgaa agactattag cattatactt aaatttaaat ggcaataata aattatttat
      421 aaatgaatta ttcccagttt cagaagattt aggtgttgaa atggtttggt ttaatgatac
      481 tcaaaatcca gtagaaataa taactgaaca tatagacaaa aatgctacta tgggtgttga
      541 taaaaactgg ccagctagat tcttattaaa cttaatagaa ttaggtggag gaagtaagtt
      601 tgtaaattca tcttatataa tagatacttt aagaatgtgt aaagatgaag aagaaaaaga
      661 gttaatgaga atagcttcaa aattaaatga caaggctatg gaacaactta aagctactgt
      721 ttctggtgaa cttacagaaa aacaacttgt tggtaaatta tctaaaatat atgaagacct
      781 aggtactgat ggattttcat ttgaccctat tataggtttt ggacctaatg gagctaatcc
      841 acatggtgaa cctggaaatg cacttgtgaa accaggagat gcaataatct tagatatagg
      901 ttgtataaaa gataactatt gtgctgatat gacaagaact gtattctata aagaaatacc
      961 tgaaaaaggt agagaaatat ttgaaatagt tcttgaagct aataagagag ctgaagctat
     1021 tgtaaaacca ggtgttagat tctgtgatat agatgctgct gctcgtgact acataactga
     1081 aaagggatat ggacagtact tcactcatag aactggtcac tctataggac ttgaagttca
     1141 tgataaagga gatgtatctt ctataaatac agatactgta caaccaggaa tgatattctc
     1201 tatagagcca ggtatatatt taccaggtga gttcggagtt agaatagaag atttagtact
     1261 tgttactgaa gatggttgtg agatattaaa caaacatgac aaagaaatat gtgtagttgg
     1321 ttaattctaa aatcaatttg ataaaattaa aaagccatat aaaaatgttt taaacatttt
     1381 tatatggctt taatttctca atctatgatt tatttcaatc cattatttat attgcagtat
     1441 ttatattgca gttattaaga ttgcaccctt tgaagtatat gtccccatca ctgaaccaat
     1501 ttcacagatt acaacatctt tgtattttac atccttttcc ataacctccc tcaccttttg
     1561 agctttttct gggcaattag catgtcctat aaataaaacc ttttcttctt tatctttaat
     1621 ttttgactct actaaagtaa tcagcttttt caaactgttt ccttcacctc ttgccttatc
     1681 tataggagtt acaacgccat ctgcaacttg tattatcgcc ttaaaattca aggtatttat
     1741 aatttttcct gctattggat ttattctacc acccttaata gcattttcta aagtttcaag
     1801 tgttccaaaa aatggaggat tatctttata tttatttatt attgaaacaa tctcttcaat
     1861 ttcttttcct tcttttatta atattgctgc ttttaacact aattgtccta ttccaattga
     1921 cccactaagt gaatctataa cctcaacttt cttgttattt ccttcctttt caaacatatc
     1981 ttttgctaaa agtgcactat tataagtccc agataatcct gaacttatat taatcactaa
     2041 tacactttct tcctgacaat ggtatgcttc taaaaattta tcaggagaag gacatgatgt
     2101 ttttggtagt actttactct cactcatttt tctatagaaa gtttcattat ctatttcctt
     2161 tccagatata taactttctt ctccaaaaga tacatttaaa ccaacaactt ctatgttgta
     2221 tttttctatt atatcatcag gtaagtcaca tgcactgtcc actatcaatt ttattttatc
     2281 catattaacc tcactataag atattatttt atttattcag tcggtcgtac gaccgattta
     2341 attattattt tatatattga atgaaaaaaa gtcaatagat atagattctc gctttattga
     2401 caataaatat ccataagctt ataatgttat taatttgtta taaatataca tggaaaggta
     2461 ggttttatga aaacaaataa tatgaaacag gctattttat catctgcaac caatttaata
     2521 gccaaaaatg gagttcaaaa tactagcctt gctgatattg caaaagatgt aaatataagt
     2581 aaaggtactt tgtattatca ctacgcatct aaagatgata ttatatatga tattgcggat
     2641 actcatttag aagttataac tagtgctata ctaaactgtg taaaaaatgt caaatcaaaa
     2701 aattctcaaa tagaaatggt taacttaatc ttagagaaaa tatctactat agaaagtcgt
     2761 ggtagggtac atatgtatct tatttgtgag gcaattactt caaataatga tttaaaggaa
     2821 agaataagat taaaatatat tgaatggaga actactctta aatctgaaat tgttgaatca
     2881 ctagaaaaat ataatagtag caaaaatgaa caggatgctg aatcattttc ttttttgctc
     2941 atgtcaatag ttgatggttt agttgtccaa agtttattaa aaacagaaaa aataccttat
     3001 gaaaatatag cttctttttt agttaatcat tggatttaaa cttaatactt agataattaa
     3061 taaaagtgaa ctatttaagt tatctacttt aacagttcac tttcttattt attctcaata
     3121 atttttactt aaaacattca atttttattt aaaccttcat ggaatcacaa aataaaaatc
     3181 tcttataaat ttaaaatgtt tttatttatg ttcatgtgta tgcccatctg aatgttcatg
     3241 accatgtgta tgtgtatgtt cgtggctatg ctcatgagta cctgaaaaac catcatcatg
     3301 attatggtcg tgacctacac catttacatg ctcatgttca tgcccatgac tgtgagaatg
     3361 ttgatgtgaa tgttcatggt tatctcctcc acagcatcct tctcctccag tcccacaaca
     3421 cccacttgta tgtggattat gatgatttct tactacaaat ctttctctta acataattct
     3481 tatacctcct agtatattat tgagtacttc atgtacccca ttaactacag tatacatcta
     3541 actaggtata atctcaatac atatggcatt ttttaacata tataattaac atatataatc
     3601 ttaaagaaat ttatccttaa aacctccacc ttttacttct tcaacgttat tgacagaaat
     3661 aaaagcttca ctatccatat cataaattaa atctttaaat ttacctattt catttgcaga
     3721 tattatgcaa taaatcatct ttttcttatt ttgtgtatat gcaccttctg cctccaaaaa
     3781 agtgactcct ctacccattt tattcattat cactttggat atttcatcat atttatttga
     3841 tattaaaagt atagattttt ttctatcaaa gcaatctttt cctatatcca tagtaaaaga
     3901 aattatatac attgtaatta aagtgtacat caccaattta gctccaaata aaaaagctcc
     3961 tgcacatact acaaaaaaat taataaataa aaatgtattt ttcataggta tatcatactt
     4021 tcttttcatt atggctgcta ttatatcaag tcctccagca gtagctcttg ctttaaaagt
     4081 taaccccata cctataccag ttaatgcacc accataaata cactgtagca tagtatcatc
     4141 tattgggaaa tatttagcaa tatcttgtgt tagccccagt gctagagaaa atagtaacat
     4201 attgacaaaa cttattagca aaaatttatt atcaaaatac tttctagcta taataaatat
     4261 tggtatgttc atcaagaaac ttgatattcc ctgatttata ccaaataatt tgtataatat
     4321 aacacaaatt ccagctactc ctccacttaa taacttggct ggtgctagat ataaatttat
     4381 tcctaaggtt gatagaatca tacccaatat aataagacaa tattgtaaaa atatgttatt
     4441 atcaatactt agctttttac tttcaccata catcaaaacg cacccctttg cataataaat
     4501 aaaccaccct gcagaacaca cgataaattt acaaataaat ttatcagaag tagcttacta
     4561 aaaatattat tcatcctcac ttaaacgttt attcattaat attctatcac atgacataaa
     4621 taatttatat gatttatata gatttattta ttgtaaattt tggaatatta aaatatttta
     4681 ttgaatagat aattatatta cataaaaaat ccctatagaa atctataaac taatttctat
     4741 agggattttg gttaactaac tattctcttt atttttttat aacttttata cctgtcatat
     4801 ggtcatttaa gttttgttcc tctaaagatt tatcatctac tctgtttatt tctaaagcta
     4861 aagtttcaga cttgatatat tcattaaaat ggtcaacagc cttagcaatt tcatcatcac
     4921 cacagtattc tattactata ttatctaata cttcaaagtt attagatttt cttaattgct
     4981 gtactttaga tataaattct ctagcataac cttcattaat caactcttca gttagcttag
     5041 tatctagtat aacaaaaaga ttgttttcaa cagatacatt aaatccttcc ttagcagtta
     5101 tacctattaa tactaaatcc ttattaaatt caaaagcttc accatctaaa tcaactgtta
     5161 aagtctcacc tttttcaagt ttattagcag tttcatgagc atttaattta cttaatgctc
     5221 caacgaataa attcatctta gaacctaata atggccctgc ttccttaaag tttggtttta
     5281 aactaaagtt catatactca tctaaatcct tagcaaatat tacctcttta acattaagct
     5341 cttcttttat taaatcaact acatcactta ttgtatcttc aaacttacca tctactaaca
     5401 ctttttgtaa tggttgacga acttttattc tttctacttc tcttgaagct ctacctaaag
     5461 taactaagtt tttagctaaa tccatttttt cttctaattt agtatctact aactcttcat
     5521 tacatttagg atattcagct aaatgaacag atacttctcc tgttaagttt ctatacattt
     5581 cttctgacat atatggtgca aatggagcta tagctctaca aagaccatgt aacaattcat
     5641 aagttgtatt gtacactgct tttttatctt ctgttaattc agttgcccag aatcttcttc
     5701 ttgaacgtct tatataccag ttagataaat catcatttat aaaatcttgt atcattctta
     5761 aagttttatt taactcaaat atagctaaat tttcttctac atctttcatt aaattattga
     5821 acttagataa tatccatcta tcaagttctg gtctatcttt atattctaca aaaaattcag
     5881 ttggatttat atcatctgtg tttgcatata aagtaaagaa gttatataca ttcttcatag
     5941 tacctataaa tttactttgg atttctttta atccatccat atcaaattta gttggagtcc
     6001 atggtggaga aacatatagt aaataccatc ttaatgcatc tgctccatat tggtcaaata
     6061 attccattgg atttactgta tttcctcttg atttactcat tttcttacca tctttatcaa
     6121 gtacaaggtc tggaactaaa acactcttat atggtgcttt tcctaataca aatgttgata
     6181 ctgcaagtaa tgagtagaac caacctctag tctggtctat accttctgat atatagtcag
     6241 ctgggaacaa ttggtcaaag ttttccttat tttcaaatgg atagtggtgt tgagcaaatg
     6301 gcatagcccc actatcaaac caacagtcta taacctctgt tactcttgtc attggttttc
     6361 cacatttttc acattttaag tgtatatcat ctacatatgg tctatgaagt tcaactgttt
     6421 ctggatttac atcttctata gctttttcag ctaattcagc tcttgaacca actgaatctg
     6481 tatgaccaca ttcacatctc catataggaa gtggagttcc ccaatatcta gttcttgata
     6541 ttgcccagtc atttagattt tctaaccagt ttccaaatct cttctctcct acaaaacttg
     6601 ggaaccattt tacaccatta ttattttcta ttaatttatc tttaagttta gtcatctcta
     6661 tataccagct tggttttgca tagtatacta atggagtttg acatctccag cagtgtggat
     6721 aattatgaac tactttttct ttgctatata atttattttc agaagctagc cattttatta
     6781 tttcaacatc tagcccatct tccattacaa atctaccttc ccaaggagta gatgtaaatt
     6841 taccagtttc atctactggt tgaataactg gtaaatcata tcttttacct gtgttgtagt
     6901 catcttcacc aaatgctggt gctgtatgaa ctataccagt tccatcttca gtagttacat
     6961 aatctcctac tgtaataaaa aatgcttttt tgtcaacttc tacaaaaggc attaattgtt
     7021 catattctaa accttctagg tctttacctt tcatttcttc taaaacttca taatcttcac
     7081 ctagtacttt atcagctaaa ggtttagata cataatatac ctcatcacct tttttaactt
     7141 ttaaataatc tacatcagca tttactgtta atgaaacgtt tgatggtaaa gtccatggag
     7201 ttgttgtcca tgctaagaag tattcatcag catctttttt cttaaattta gctataactg
     7261 tattagtttt tacttcttta tatccttgtg caacctcatg actagcaaga ccagttccac
     7321 atcttgggca ataaggaagt attttatgtc cttcatatat atatccttct ttgttgaatt
     7381 tgtttagtat ccaccataca gactctatat agttattatc taaagttata tatggattat
     7441 ctaaatcaac ttcataagcc attctctcag tcattattct ccattgtttt tcaaatgaga
     7501 aaactgactc tctacatttt tggttaaatt cagctattcc atattcttct atttgttgct
     7561 tgctagtaag acctaactct ttctctactt gtatttcaac tggtaatcca tgtgtatccc
     7621 aaccagcttt tctctttact tgataaccag acattgtttt atatctacaa actgaatctt
     7681 taagcgttct agataaaaca tggtgtactc caggatttcc attagctgtt ggaggtcctt
     7741 catagaatac gaaacttggg tcatctttcc ctttttctaa agttttttca agtatattta
     7801 tgtctttcca ataatcaaaa acttgtgctt ctgcttgttt tacagaactg tctaccaatg
     7861 gcttaaattt cgccatgtaa atcacccttt cctactaaat attataattc caaaattttt
     7921 tattctttca cataaattta aatactaaaa aactcgcccc ctttaaacca aagggacgag
     7981 ttataaatcg cgttaccacc ctaattctat atgtaaacta aatactagct acatataaca
     8041 ctctaatagt ataacggtac taaccggcac agcttagtag aatttaatcg ttcagcctgc
     8101 agctcaagag tgatttttat ctttataaat tcattggttc tcagcatacc caattctctg
     8161 taaaattatc taaagataaa tgtctctatc aacgctttta aaaatttatc tgtgtttata
     8221 tgaaattcta taacaaataa tttgaatagt caatatattt ttgaaaaata taataatttc
     8281 tatttttctt acatttttat cctattttta atctttgtta tagatttctc aaactcacac
     8341 aattattcta cataaaaaat aaacaaatta caagcttatt ctaaagttgt agcagcttca
     8401 tcattactat aattatacac tgttgttcct tcaaatgcgt ttacacattt ttcatctaca
     8461 tcataaaaaa tctcatcaac acttcttatt tcatcttcta acagtgattt aaatttattt
     8521 ctaaatatct taaattcttt gactaaagca tcatactctt ttcttatctc tataacacgg
     8581 ttattagctt gttctataat ttgtctagct tttaaatcag cttcttcaac aattatctta
     8641 gcctttttat tagccgcact acaagtgtct tctgctgcac tttgagcagt tattaaagtg
     8701 gcttttagtg tttcttcaat attttcatac ttgtttactt ggtcttgata taaccctaat
     8761 ttttctttaa gagctgtatt ttccctacat agagattcat aatcttcctt gatgatatct
     8821 aaaaattcat caacctcttc atctctatac cctcttaaac cttttttaaa ctctttattt
     8881 tctatctcaa ttggagttag cattacattt cctcccagct ataatttaaa ttagtcctta
     8941 tacaattaat cttgcttgaa cttttatttt tcctttttta gtaatatctc ctatttgtgt
     9001 taccttagct cttcctttgc ctctaataga aatcaaatca tcatttttta ctattttaga
     9061 agttgaagtt atcttttcat aattgacatg gactttttct ccatttatgt attttgcact
     9121 atcttgtcta gatatattgt aaataccact tatcacacaa tctaacctat ctgaagatac
     9181 tgtaaatgat acttctttat attttgaaga actattaaca atattttctc tggaaatttc
     9241 ctttacaatt acattatttc ttgaaacctt ttctaagttt acaattataa aatcgcaaat
     9301 atcactactt acaactactt gacaaaagct atcatgtata attatatctc caattttttc
     9361 tctttttata cctagaccca gtattgaccc taagtaatct ttatgagata tatttttaaa
     9421 tttgaaattc ccttctattt gtaaaaatct aagagtatct tctatatctt catattccat
     9481 gtaaaatgga taaataaata cagtgcttct ttcagctcgt tcataacctc catccacact
     9541 gtattttatt tcatttgtag aatttaatat tgctacagca tttttcactt catatggatt
     9601 aagaaactcc gtagacttaa catcatagtt ttttatacaa ctattagcct tgtctatcac
     9661 tttaaacatt ttatttttca agtctatatc ctttatgtga tttgttagtt ttaatttatc
     9721 catcctatca ccttagaata taaccaatac taattgtctt aggaacatca ataatactat
     9781 tgcaattaaa ggagaaaaat ctataggtcc atttgtatat ttatacatta tagaccttat
     9841 tggcccttct attggctctg taagattttc caaaatatca aaaattttac tttgtcttcc
     9901 atttggaaac caagtcatca agctttttat aactatcatc catgctatta tatctaaaag
     9961 ataataaagt gcaattctta ttgttcccat atattaaccg cctttttact atttattttg
    10021 ccaagggaag aatgctttac tttctaattc ttttttcata tttgcatcta tatccacatt
    10081 attaggagct agtataaata ttgatttaga aaccttttgt atacttccat ctaagacata
    10141 aactgcccca ttcataaagt taaatataga tttacgaact tcagaattag tcaatccttc
    10201 caaattcact ataactgccc ttctttgttt tagatggtct gctatagttg cagcttcatc
    10261 ataaacctta ggttcaacta ttacaacttt catttggcta gttgtatgta aatttacaat
    10321 tttatttgct tttgtagtac taaagccact gttcatttct tcttcttgaa ctatatcgtc
    10381 catgccactt tcatattcat cttcaatgta atcttcttct tcatcaacta tccaattctt
    10441 aaatttagat attattccat ttgacattat taacttcctc cttaaaattt attcaaattt
    10501 gagtaattat tttttattgt agtttctttc tccaaagatt gatgttccaa ctctaacaat
    10561 ggtagcccct tcctctatag caatttcata atcgtgactc attcccattg acaaaacatc
    10621 catccctaca ttagggatgt ttagttcttt tatttgtttt gataaatctt taagaccttt
    10681 aaatacacct cttatctcat cttcatcttc tataaaaggt gccattgtca ttagaccctt
    10741 tatttttata tttttatatt tttctgacac ttctttgaca aagtctacaa cttcttctct
    10801 atcaagtcca tgcttagact cttctttaga tatattcact tgtactaagc aatctatttc
    10861 tctgtttatt tgttctgcac gtttttgaat ttcttcgcat aatgcaattc tatctaaaga
    10921 gtgtatcata caaactttat cgattatgta cttaacttta ttggtttgca aagttcctat
    10981 caggtgccag tttaatttat cacctataat atcatacttt ctagctaatt cttggggttt
    11041 attttcacca acagaagtga ttccttccct cattgcctca ttgactaagt ctacatcaac
    11101 agtctttgtg actgccaaaa gggtaatctc tttgctgtct ctatctactt tttttgcagc
    11161 tttatcaatt ttgtttctta tctcagacaa attatcttta atagaaccca ttattatcac
    11221 ctactttatc cttatatttg tgtttatact gttaggtctt aaaattattt tgtcatatag
    11281 attaacactt gtaactccat ttatgtcatt tttataataa tccacaaata cagaagattc
    11341 attctcataa gttattcctt ctaatatgac aaattttaca ctttgtgatt gttcatcaat
    11401 tacatataca ccacgtttat tgtcttttgt ttttatagaa gttttaggta tttttaaaca
    11461 ttccatctga ttatatatta tatcaaattc ttcgacactt gtatcctgat ttgcaacaat
    11521 ttggttactt atttttaatt ttaccattat tttattatta ttttcatata ttttgtaaac
    11581 ttttgcatct aatttttgct catgatattc aattattaca ttttgatttt tttcaaagtt
    11641 attagaatct tctttactga tgtatgtaga tagataatta ctctggtcat tggcatcttt
    11701 ttctatactt ccttttacat gaatctttgc atcaactttt tcatttttta acaccaacgt
    11761 atttacattt ttgctaatta tcattactaa tatattaaat actaaatata taagaatacc
    11821 aagtatcact aatctggtat attttacctt tttcaaagac tctcccccta taaaattact
    11881 ttcattataa gtacttcttc taaaaaatgt ttattccttt tttataagct aatattttac
    11941 gttctaataa ttattttttc catattaaat ttatttttta tcaatcttcc tatacaagac
    12001 tgcattattt atatataatt gtattataaa gtataagttt aatttacatt ttatacgtta
    12061 agcttacttt atatgttttt atagttattt ttatttttag atacaatata aaaccaatta
    12121 agatttaaaa taacttttgt tatgttgaca tttatttagg taatcattta cgaatcagga
    12181 taacgttatc ataacaaaag tttatgtcct aaaaaaaata caaggaggat tttcttatgt
    12241 cagaaatttt attaggaaca ggtttacttt tattggttct atgttgtttc acaattttta
    12301 gttacaaggc cccatatggt atgaaggcta tgggagctct agcaaacgct gcatgtgcta
    12361 ctttcttagt agaggcattc aacctttctt tacttggtga tgttttaggt atggaatttt
    12421 ttagaagtgt tggagctgct aatggtagtc ttggtggagt tgctgctgct atacttgtgc
    12481 ctttagcctt aggtgtatct ccagtttatg cagttttaat aggattatct tgtttagatt
    12541 tttctatatt atctggattc gttgctggat actttgtttc tttcttaatt aagtttatag
    12601 agaaaaaagt gccacctgga gtggatttag tggttgtaat tcttctatgt gcaccactat
    12661 ctcgtcttat agcatcaaat gtagacccat tagttaatag tacactacta caaattggac
    12721 aagttttgat agaagctacc aacattagcc ctataataat gggaattata ctaggaggtc
    12781 taataacagt agttggaacg tctccactta gttcaatggc tcttacatct ataattggtc
    12841 ttactggtct tcctatggca ataggagcat taggtgcttt tggttcagct tttatgaact
    12901 caacactatt tgcaaaattg aaattaggtt ctaaaaaaga tattatatct gttgctattg
    12961 aaccattaac ccaatcagac ataatttcag ctaatccaat acctatatat tctactaatt
    13021 tctttggagg agccttttct ggtatcattg tagccttgat gggtcttgta aacatgacac
    13081 caggtacagc tacacctgtg gctggttttg ctataatgtt tgcttataat ccagttcaac
    13141 aagttcttat aacagctggt ttatgtgctt tattcaatgt aattgcagga tttttaggtt
    13201 caaaaatatt cagtaccttt aaagttctta ctgctggtga aatacgtggt actgatgtag
    13261 aagaagaaat tgcttaaaaa acaataagat ttataaattt atatctcaag tagtagagtt
    13321 ttatattgta ggcaatttta ttaattgcaa acgttttatt ttttatttat gtttatgatt
    13381 attatgtgtg ttgaatattt agataaattt ttatttatta tttttattta gatttttgtt
    13441 tagtttttta tttggttgat atattaggaa taaaaattag tatagtttat tactagttag
    13501 atagcataac tttttagata cataaaaaat tttatattaa ttagatatta ttaaaataaa
    13561 agcgatacat gaatttttaa atgtccatgt atcgctttat ttttgccata atcttaacaa
    13621 ctaattttta aagtttatta tcttaacaaa gttcattatc ttatataata tttaaatgca
    13681 tagacttctt taatttaacc atctaaatat ttaattaaat attattatat gtaatataac
    13741 taatttttaa atgaatgtat aggagctgga atccttcctc ctctttgagt aaataattca
    13801 gatgaatttg tatttatagg cattactggt gctcgtccta aaagtcctcc aaattctacc
    13861 atatcaccta catcacatcc tggggctggt ataattctaa cggctgtagt cttattatta
    13921 atcataccta ttgctgcctc atcagctatc atggctccaa gcgtacctgc tgtagtattt
    13981 cctggcacag ctatcatatc aagacctaca gaacaaatag cagtcattgc ttcaagctta
    14041 tctatactta aagcaccttt aataacagaa tcaatcatac ctgcatcttc agatactggt
    14101 ataaacgctc cactaagtcc tccaacatgt gaacatgcca ttactccacc ttttttaaca
    14161 gcatcattta aaagtgctaa tgctgctgta gtcccatgag taccaacaac ttcaagaccc
    14221 atttcctcta atatatttgc tactgaatca ccaatagcag gtgtaggagc tagtgataag
    14281 tctattatcc caaatggaac atccaatcta ctagaagctt cctttgccac aagttgtcca
    14341 actcttgtaa ttttaaatgc agtcttttta actgtttctg aaacaacatc aaaaggctct
    14401 ccctttactt tctctaaagc tctcttaaca acaccagggc cacttacacc aacatttata
    14461 attctatcag cctcaccaac accatggaaa gctcctgcca taaatggatt atcttctact
    14521 gcattacaaa atactactaa ttttgcacaa ccaaaacctt ttgcatcttt tgttaattct
    14581 gcagtttctt ttatgatttc tcccatttct ttcactgcat ccatatttat accacatctt
    14641 gtagaaccta catttactga agcacatact ttattagtac ttgctagagc ttttggaatt
    14701 gacttaatta gtatcttatc accttttgta tatccctttt gaactaatgc tgagaatcca
    14761 ccaataaaat ctatacctaa atcctctgca gccttatcca atgtctgtgc aaactttaca
    14821 tagtcatttt catctgttgc acctgcaata atggaaattg gagttactga taccctttta
    14881 tttacaattg ggataccaaa ttcacgctct atatcttttc caaccttcac caaatcttta
    14941 gcagaactca taatcttatt gtatattttt tctctagctt tatctccatc agggtctata
    15001 caatctaata aagaaatccc catagttata gttcttatat ccaactgttc ttcatctatc
    15061 atctttattg tttcaagtat atttcctaca ttcactatta tcacctcccc ttactgtcta
    15121 ctacagatta tgcattgaat taaatatttc ttctcgctga atttttacat ccactgataa
    15181 tttcttacct ctttcaacca agtcctcaac cgttttctca aaagaattta atgatttctc
    15241 taaatcaact atcataatca tagtaaaata atcctgtaat attttttgtg atatatctac
    15301 tatgttgata ttttgcttgt ataattcatc actaattcct gccactatac ctggtttatc
    15361 ctttccaatt actgttaaaa ttgctttcat aataatatcc tcccttattt ttttataaat
    15421 cttatatttt tacaaattga actatgtgtt aacatatatt tcatttattt gttagtaaac
    15481 cactttttat gtatatattt aatatatttt aatacctata cattcatttg tcaatttttt
    15541 ttcaatattc tattattttt atactattta gaaaattcat atattatata tgttttctca
    15601 acaacaaata cagttttcat aataatgaaa ggtattactt agcaatctca tgattttcta
    15661 aataatacct ttctaaaaat tattttttta attttatgaa tttaattata ctttttctag
    15721 tatcaggatt tacaattaat atagaaccaa tcataattgg tgttagtata caaaataaaa
    15781 aaataatatc tttttttaga ttatattctt tagattctaa tttataatta ttatttatta
    15841 ttccatcata atcacttata tttgacttat ttttatttgt atcttgattt ttgttagaaa
    15901 tactttccct ataaggaaaa gaatgttcat tggtaccaaa tactgatttt gagcttaaat
    15961 cataattatc atcattttct gtaattgaat ttttattgta aaatcctttt tctttatcag
    16021 aattattttc tttattacca tctttaggta tatcatgagg atttttattt tcattgctat
    16081 cagtacttga ttctacatca tctttaggag tatttggttg tttctcatca gaattatcat
    16141 tttcattatt attattttca ttattattat tttcattatt attgttttga ttgttattac
    16201 tatcattatt ttcatttcca ttattcccat cttgattgtt atcatcctca tctctatccg
    16261 ggtcgtctct acctcctcct ccacggtcac catcaatatc atcattttcc cgttcatcat
    16321 ctggtgtctc ttggttgtct ccgcctccca ttataaatcc tggtttaaaa actatatttg
    16381 aatacccttc ttttaatcct cctacaactt ttactctaaa acaacgcaac ttaaaatctt
    16441 caaaaaaaag tatagcttga tctctaatat cctcctgttc ttcagatatc caattgattc
    16501 catcgcttga atattcaaca aaaaccttac ttgcactata cggcatatca taaaataaaa
    16561 cagctacata tccatttttt gtattttgaa attccagttc caaattgtct attggttttt
    16621 tctctataac agatacattc aaaataggaa ctacatcatc atttattata attttttctt
    16681 tatttaacat ttctgtttta aactttcctg ttatattaaa attttctccc ttttccaact
    16741 tttcataata atcagtaaaa tcccattcta catctatatc aagtcgatta ccactagatg
    16801 tttctaatac catatatttg ggaaatttta tattttctat tttatcatct ttacttactt
    16861 ttatttcatc actaaataca ccaattcccc tatgacaaca tttcacaata tcatagccag
    16921 aatcttcagc tatagagggt ataagctcat tataatcatc aattatataa actttcttac
    16981 tttctgatat aattgattgt gccaacttat tattatctct tagtaaccca ctttcattgt
    17041 tgctactata tgcttttatt gaagtttctt ctatctcaac atcaccttta gaataaacac
    17101 ctatagcatg ttctccattc acttcaatat ccttatttag tattctaata tcattttcag
    17161 tataaattcc tattgcatct tttccatttg cctttatttt ttcaacagat tcgtaggatg
    17221 atttaatatg taagtctcct ccttcttcaa cgtataaggc tgtcccatca gtagcaatta
    17281 tgttgtttat accaatactt atacttccac cattttctat atgtattact ccttcttttc
    17341 ctccttcacc tattatattc aattcattat catcaataat aaatctccca tgatttttta
    17401 tcaatatttt atatttgttg gtatctattg taaaattact gtcataagga atataaaatc
    17461 taaactcttc atctattacc atatcacaag aaagagtata aactatttct tctttattat
    17521 ttttattttc ttgaacccac ctttcaaaat cttcaagaca attttcctct tgtccaaaaa
    17581 ctttatgtgg aaaacttata aaacacaaaa ctattatact cataaaaata aatatctcat
    17641 actttttcat cttacactcc tagaaatttt tagctttgaa acaatcctgt cgaaacatat
    17701 acttcaccca ctataatttt tccacaatta tcaattattg tcaatttata taagtatctt
    17761 tattaattgt tataaataaa aagctactta ctaaagcaaa cagacgtttc actttaataa
    17821 gtagctatat ttttataaca aaatagttat aatattagaa cttccttcat ttacaatttt
    17881 ttgtaaagtc ttttgcattt taactcttat ctcttctggc atagtatata atttactttg
    17941 taattgctct tttactaagt catgtagtga tttaccaaac atatttgatt cccataaatc
    18001 tgctggttgc tcttcaaata cttccataag atattttatc atctcttcac cttgattttg
    18061 atttccaact attggagaaa cctctgtaga tatatctgct tttataatat gaagtgatgg
    18121 tgcattggct tttaacttga taccatattg ttttccttgt ttcattattt ctggttcttc
    18181 cagactaagt tcttctaagg aaggagctac tactccatat ccttttatct tggcatctat
    18241 taaagcactt tctattttat catactcatt tttaacttta gatagtctag ttatcagact
    18301 aagcagttga taatctcctt caattttaaa tccacttttt tcttcaagga cattatagaa
    18361 taaatcttgc tttgtctgta aatcaatatt tataacacct tctcctagct ctacattatc
    18421 aacacctgta tcttccaaaa actctaactc agaaaatcct tgtataatac cttctatatc
    18481 ccttatttta cctatatcta taatactttg ttttaatgtt gttattatgc tactttttat
    18541 ccaatgattt ctttcaagac cttcaaccca ttttggtaag ttgattctta tttctgtaag
    18601 tggaaaatca tataatacag cttccataac ctcctctata tcttcttctt ccatttcaac
    18661 cacattcatt ggaagaactg gaacttcata cttttcttcc aattcacttc taagcatact
    18721 cgtttcctca ctttttggac ttaacgtatt taaaaccact gcaaatggtt ttttaagatt
    18781 tttcaattct tgtataactc tctcttctgg ttctacatag ctttttctgt caattccagt
    18841 aactgaacca tctgtcaaga caactatccc tattgtagaa tggtctttta taactttttt
    18901 agttcctatc tctgctgcct tttcaaatgt catagcttct tgtgaccaag gagtggatac
    18961 taatctttgt tttccacctt cctcatgccc taaagctcct tcaactatgt atccaacaca
    19021 atctaccatt cttactttta aagatactgt gtcttttatt tttatttcta caccatctgc
    19081 tggtacaaat tttggttcta ctgtcattat cgttttacct gaaccacttt ggggtatctc
    19141 atctcttgtt ctatcctttt taaattcatt gtctatatta ggtataacca acttttccat
    19201 aaattttctt ataaaagttg attttcctgt tcttacaggt ccaactactc caatatatat
    19261 atccccctga gtccttttgg atatatcttc gtatatgtta ttattcatat tattcctcct
    19321 atacatctaa tcttattaaa ttatatttag agttaagggt taatattctt aaaatagaaa
    19381 aataaagacc agcaatatta atttaattta catatagaaa tttgtaaatt tcaatgtaaa
    19441 ttaaattaat attgctggtc caaatagtag tgtattatat taactgactt tagtagtatt
    19501 ttcatttgaa ttattattca tctcaataag ttctttacgt ctttcttttg tcatgttcat
    19561 ccatataaat gtaaacacca atccaattat ggctactata gtaagaacta tgaagtagta
    19621 cctatatcca gttacacccg gaaatgcatc caatatcttc cctgctgcta aaggacaaag
    19681 tacttctgga agatatccta atgtagcaac tattcctata gcagttcctg atattgataa
    19741 tgaataatta ccttcttcaa gtaacgaata gtgtaaacct tgaattgcat acatagatac
    19801 atatattgca acacatccaa tcaatagtat ataaatcata gatgattttg ttggtgtaaa
    19861 tattacacct aaaagacctg caatcataag tataaatcca taagatataa tctttgatga
    19921 accaacttta tctccaagta atccagaagc agctgatgct actggtctac aatactgagc
    19981 taatattgag attgcagatg caaacaccac agtagctcca aatgcttcag ttgaataagg
    20041 cgtaaaataa taaaatgaca tattagctga atatgaacaa aacattatta ttgttattat
    20101 ccatgtatga ggcattttta ttactgcaaa aaaggcttct ttatcaaatt ttgctgatac
    20161 ctctttttca ttatccttta atttgaaaaa tactaatact ccaactatta catttattgc
    20221 cgagtataat actataatag ctgtaagacc agatttatca cttacttttg atgaaatata
    20281 cccaaatagt atcaatacta gagatagatg aattgcattg gtaattccac gaccactttc
    20341 cataaatcca aaagcttttc cttgttcatt ttcagatgct aacattctaa cagcttttac
    20401 aagagctggc caaaaagtaa gtattgttgt tattccccat aaggcatgga ttaaaaacac
    20461 aacaatatat ggaggataaa gtgttaatac aagaccagat attcctgtaa taattaaaga
    20521 acctgaaagc aattttctgg ctgaaatacg gtctgctaaa aaaccaccaa aaaggtatga
    20581 taccattgca agtagaccat aaatacttcc taagcttccc atttgtgtat ttgtaagatt
    20641 aaatgcttgt gcaaaagtat cataataata attctttaaa tatggtaaag catatataaa
    20701 tgctcctgct acaaagagta ttatcaagac tttgaaattt ttccaaatgc ttggtttatt
    20761 tacttcaact gacatacttc ctccttatgt aaatacctgt aaattttcat gataattaat
    20821 tattgcttat tgtattgttt attcaatatt tcggaatatt tattaatata ttaggttaat
    20881 ctatcaataa tttatggtga gttatgagct aaaagtaaac tcttttagct caccataata
    20941 attatatttt tatttatact aacgtccaga gttattagat atcttttttt catagttctt
    21001 ccatagcatt aaacctgtta ttataacaaa aagacctcct cctatcattt caatctcata
    21061 gattatatta tcttttaaag tctccataac tggtgccgtt gagaaaagta tgccaactac
    21121 agttactaca agtaccattc tggcaatatt gatacaaact gaatcacttt ttgcaattga
    21181 atatggtctt tcttcatttg gtcttgtctt tctaagtcta atataagatg taaaaagcaa
    21241 gacatatgga aataatgctg ttaatgctgt catagtaaca agaacattgt atatattatc
    21301 tactgatggt aataagtttg ttcctacaag aagtagactc actattatag cctgaactat
    21361 tactgcattt gatggtatat tgtatttatt tgtaacagtc aattgcttag gaaatacacc
    21421 ttctcttaca tttccaaaaa gcatctttac tggacctgct atataaagta ttaatgctcc
    21481 tagtactgat aaagttattc caaatgctac tattcttata aaccaacttc ctatacctaa
    21541 atcttgtgca actttagcta atgcatctag tatccctgta gatgctgcaa ttttatctgg
    21601 aggaagtatc attgttattg ctactgaacc taagatatag attcctccta ttaaaacagc
    21661 cacagttaat atagctttag gaaagttttt cttagcatta tccatttcgg ttatgaaatt
    21721 agcagtcgtt tcagctcctg ataatgcaaa tattatagca gatatagaaa ctaatgaatt
    21781 ggcatctatt tttggtatta tattttggat tgtatatatc gatgctgatg gagctttttt
    21841 gagaataact acagacataa atgccatcac tataagtaat atagcaggaa ttgtagaacc
    21901 taatgctcct gtattcgtaa aaaattttcc aaatgccatc ccacgagtac ttactaagga
    21961 caacagccaa aatattatga gtgaaagtgc taaaataaac atcttattgt ttgataaatc
    22021 tggatttcca agcatatatg caatatttat cgctaaaaaa gtcaagaaag aagaatacca
    22081 aaatatcttt gatgtccaat ttagccatga caccataaat ccccacttct ctccataagc
    22141 ttgtttaacc cattctccaa gtccaccatc ttttggatat gttgcagcca actctgcaca
    22201 tataagtgat gcaggaacaa agaatataaa agcaaacaat aaccaaactg gtatagaacc
    22261 aagacccaat ccaaaactag aggcagtaga tttagctatc catctaatcc caaatagtgc
    22321 tgatatattc ataaatacta aatcccaaaa tccaattttc tttttcactg taattcctcc
    22381 aatttcaaat ttattcagac tcatacacaa tgtattgact taaatatcaa gtataagttc
    22441 ttcaacactt cttggataat tagttataat tgtagcacca ttcttaccta ctattgctgt
    22501 atctgagtgt ctaaatcctc caactccagg tatatatatc cctggctcca tagaaaatac
    22561 cattccttct tctaatatta attcattgtc aaatcttaaa tatggctctt catgttctga
    22621 taaacctaaa ccatgaccaa ttctatgatt tgaatataac tctagaccat actttgcaac
    22681 aacagctctt gctgcttcat ccaccattct tgctggtata ccagctttta ttgtatctaa
    22741 tccagcttgt tgagcctcta ctgcaatctt aaacacttct ttttgtcttt ctgttggttt
    22801 gcctataata aaagttcttt cattttctgc tctatagttt tcataccaaa cctgtctact
    22861 atgtattact atatctcctc tttgaagtat tctagtattg gaatctaaat gaggttgtgc
    22921 tgttctatca atccctgaac aagtccagtt tgcaaatcct atatatgtat caggataatt
    22981 ttcagaaaca tattttagta aagcattatc ccctgcaacg tcaaactcca attcactcat
    23041 cccaactcta acattttcta aagacccttt tattcctata tcagataggt aacctgctat
    23101 cttcaaaaat tctatttctt ttgcatcttt tacatatctc atttcaaata ttttacttcc
    23161 aacatctttg atagcaaatg acttatcagt tatatactct gaaaagcttg cagatactat
    23221 atctttttct attccaacag ttgttccttt agggtattta tttaaaatta tatctaagta
    23281 atgtttatgt gatattccat gttctttcat ttctggtatt tcataataca catatacatt
    23341 atcaactttt gcaacttctt tagcatgtaa ttcttctaat ccaggtacta ttaattctat
    23401 tttatcttga gttactactg ttacaattgg tctagaataa gttattgctt taaatccact
    23461 aatataaaat tgattgtctg gttcaaatat taaagcaatt tcaatgcttt tttcattcat
    23521 gtactttctc aatctttcta gtctcatatt atttgacata atgcacctct ttcttaatat
    23581 tcagataatt tattcaatac aacaaggatg aatatattat tttaaaattc ttaatatttt
    23641 tagtacttgt cgtatttgta tgtaaattaa ttttatttgt gattatttta tcataatcat
    23701 gattattttg tcaatcatga ttttatttta aaaaattttt tatattctag tgattttaaa
    23761 ttatctaaaa tataacgagt atacattgta ttttgtaata ttattcttga tttatggtaa
    23821 gtttaatgat aaaatcacga ttatataaac tatattgatt atttgtaggt gaataaaatg
    23881 acaaataaag aattacaaaa gaaaaggata ttgatgtatt tcatagaagc tgctcaaaat
    23941 attatggaaa acgaaggtat agaaaatatt acacttcgta aggttgcaga tatggctggc
    24001 tataatagtt caactctata taaatacttt aaaaatttag accatttaat atcatttgca
    24061 tctataaaat attttaaaga ttataacttg aatatttcaa gatgtataga aaatgtcaat
    24121 gatgaatata agaaatatat aattatgtgg aaattatttt gtaagcattc ttttgataat
    24181 gctcaagctt tttaccaggt attttttaac ttgtctagtg atgaacttag ctatataaca
    24241 aaacaatatt atgatatgtt tccagaagat ttaggtattc atgatagtga catatcttta
    24301 atgattacag gagaaagtat aaaggaaaga aataagatac tattaaacaa ccttgtagat
    24361 actggatata taccagctaa agatttagac ataatcaatg atataattat ttctttttat
    24421 caaaatctat tattcttaaa aaagaacaca ggaaaaaatt ctgatgatga tgaattaact
    24481 atgaaaatga tttcttctat tgaatacata ataaaacatg ctaaataaaa ttaaaatatg
    24541 gctgtctcaa aatagagata agcctttttt attgttgaaa taataaaaat atatttaatt
    24601 tcatataaaa aagagtgcct cgtaaactaa aaagttcatt ttgagacacc cctaaaataa
    24661 atttattatt tattatacaa tagtttatat atcatcacct aaaactactt cttccatttc
    24721 atgcttttta cttctcatca ttagttctat tccaacttct ttaggatttg aacctttctt
    24781 tattacagaa tatatagcat tagttatagg catatctata tctaattttt cagaaacatc
    24841 atgtgctact tcagtagcag ttatgccttc tacaaccatc tttacttctt ttaaagtatc
    24901 ttctaaactc atacctttac ctatgagtat accagctctt ctatttctac tatgcatact
    24961 tgtacaagta actattaaat ctccaatacc tgaaagacct gcaaaggtag acatattagc
    25021 tcccatagca attccaagtc tgctcatttc actaatacct cttgtcataa gggcagcttt
    25081 tgcattatca ccatacccta atccgtcaca tatcccagct ccaaatgcta taatattttt
    25141 taatgctcct ccaagttcta ctccaactat gtctggattc gtataaactc taagtttagg
    25201 gctcataaat aaatcttgta tcatctgtgc tatttttaaa tctttagaag caactactac
    25261 ggtagtagga atatcccttg ctacttcttc agcatgagat ggcccagata gtattacata
    25321 tggattttgt ggtaattctt cttcacagac ttgagataat ctaagcccag tccctttttc
    25381 aagaccttta gctacattaa caataacttg tccttctttt ataaatggtt ttattttttg
    25441 gcaagtactt cttacagcct gtgatggaac tgctaaaact atcaccttac aatcttttat
    25501 agttttttct atatcagtac ttatagttat attatttgga aataatactc ctggtaaata
    25561 gtctgtattt tctttagttc tgtttatttt tttagcttgt tcctcttttc gagtccacat
    25621 gctcacatca ttaccttttt ttgcaagccc taaagctaat gcacttcccc agcttccagt
    25681 acctaataca catactttct ccatgtttaa caccacctga gtataatata tttactattc
    25741 tttcttttga ccaatttttc tctctgtccc atttaataat cgctttatat ttgctctgtg
    25801 attatatatt actgatgcag ttaagaacag agctactatt aaaccagctt tattttttac
    25861 cataatcata aatattggtg aacatcctat tcctacaaca gaacccaatg aaacatactt
    25921 tgttatagct accaccaata taaatactgc tagacacatt agtgttatta ctggatttac
    25981 agctagcatt gcccctagtg aagttgcaac acctttgcct cctctaaatc ctaaaaccgc
    26041 tggccaatta tgacctgcta caacacatat aactgccaga tatccagcta aaagagtatc
    26101 tattccaacc aatcttgctg caaattctga tattaaaaca gccattacac ctttaagaac
    26161 atcacctaaa aatgtcattg ctcctgctct ttttccaagt gtcctaagta catttgtcga
    26221 acctgcatta ccagaacctt gagtccttat atcaacccca gcaattcgct ttgcaactat
    26281 gtatgaggta gatatatttc ctaaaagata tgcaactaca gcaataatta tataactaaa
    26341 tatctccact gaatttcccc cctttagtta tattttaaga tatttatttt ttattttttt
    26401 gtctatactc aaactttatg gaagttcctt caaatccaaa attttctctt atcttattct
    26461 caaggtatct ttgatatgag aagtgagtta aatccttatc atttataaac aatgtaattt
    26521 taggaggtct aatgtcagtt tgagtcccat agtaaatttt taatcttcta cctttgtcag
    26581 atggtggttg atttaacatt acagcttctc caattacttc attcagtgct gaagttgata
    26641 ttctcttaga atgttcattt gacacatatt caactgtatc tagtatcttg ttcattcttt
    26701 gattagtttt tgcagaaaca aatacgattg gagcatacat cataaatgga aatttttctt
    26761 ttatatcttt tgtgtaatta ctcatagtct tattatcttt ctctattaaa tcccatttat
    26821 tgattacaaa tatgcatcct tttccttcat catgtgctat acctgcaacc tttgtatctt
    26881 gttctgttac accttctaat gcatctatta ctatcaatac tacatctgct ctatctacag
    26941 cactcatagc tcttattaca ctgtattttt ctattgtctc atatatttta ctttttcttc
    27001 taagccctgc tgtatctatt agaagaaatt tctgtccatt tttttcaaaa taagtatcaa
    27061 ttgcgtctct agtagttcct gcaattggac ttacaattac tctttcttct ccaagtatct
    27121 tattgagtat agaacttttt cctgcatttg gtttacctgt tatagctact cttattatat
    27181 cttcttcata ctctgtatct aatcctgctg gaaaattttc aactatctca tctaataaat
    27241 ctccaaatcc catactattg gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat
    27301 aaaaatcgta tatattatca aattgacttt gactatcaat cttattaacc actaagatta
    27361 ctggtttttt tgtctttcta agcaattgtg caatttcttt atcagcagct gttattccag
    27421 ctttaccatc aactataaaa agtattacat gtgacatgtc cattgcaagc atggcttgat
    27481 ttctcatttg agataatatt atatcttcgc tatcaggttc aattcctcct gtatccacta
    27541 atgtaaaata tttatctaac cattctactt cagcaaatat tctatctctt gtaactccag
    27601 gtgtattttc aactattgat atcctttttc ctgcaaattt attaaatatt gtagacttac
    27661 caacgtttgg tctcccaact acagctacaa ctggcctact tatactcatt ttatttctcc
    27721 ttccatcttt tgttttctat tttaaaattt tatccacaaa atcttttcct tcattcaaac
    27781 aagggataat ttttattctc ataagttctt ctaattgatt taattcaata ttatctaaga
    27841 aaatttcttc atctgctttt agcatacttc ttgttatata aagagcttct cctaattctt
    27901 tatcttcaag ttgttctttt aaatccttag ctgttaaaag acctgataca gttattgtct
    27961 ctccaaaaaa cttgtttttg atttcatata catttacttg aagattttta aacttgtcca
    28021 tcatagcatc tgccatactc tgtataaatt catatgcaga atgaccagta gctattgata
    28081 ctttcttctc tctttttaga atattctcag gcaatatatt tagatactct tcaatctctg
    28141 tttttaactt acgtatcata cctacaccat cttcaaactg taaaaaacct tcatattctt
    28201 catatccagg caattcacta ttagatagta tataaaattc atctgataaa aatgcaaatc
    28261 gagtacctaa tttttcaaga tatttctgtt gtatttgatg tatttgctct atagttttac
    28321 ttgcgctctt atcattaaat atatttaatt ctactagatt ttctctatgc tttgtaatcc
    28381 caactggaac tatcgcaaca ctgtttacat atggataaag ttgagctagt tcttttattg
    28441 ttctatccaa ttcctttcca tcatttatac ctggacataa aacaatttga cagttcattt
    28501 ctatatgagc atctgccaat ctcttcatta taccatataa ttttcctgca aatttattgc
    28561 ttatcatttt ttgtcttaac tctggattag ttgtatgcac agatatattt ataggactta
    28621 ttctatattt tataatatta tttatatctt cttcactcat atttgtaagt gtgacaaaat
    28681 ttccttgtaa aaaagataat ctcgaatcat catctttaaa ataaagagtt tctctcatac
    28741 cttctggcaa ttggtctata aaacaaaaca tacacttatt tctacagctt ttagcttggt
    28801 caattatcgg atttgtaaat tcaacaccca aatcttcatc atagtctttt tctatctcgt
    28861 atatgtagac ttcgccatct tttttttgta tttctacatc taaatattca tcacttagta
    28921 agaatctata ctctattata tcatgtattg gctgttcatt cacagatata agtaaatctc
    28981 ctacctctat acccatttct tctgcaatac tatctttata aaccttactt ataatattat
    29041 ttactttttt tacatttact tccaatattt tcaccactct tttgtattca tcaatgctta
    29101 ttataatgta ttttaatatg caatttattt atttgttata ttttatgata gcatttttaa
    29161 gcatttcatg tcataataac acattttaat tctaatagtc aatataagta aatagctata
    29221 aaatagatta gaacaaataa tattcaaatt taagtttctt aaaaatttga atattatttg
    29281 ttcttaaaaa aattttataa atttaatgtt ttactatgat taacgtccca tttgtcatat
    29341 catgaaccat tccacctaca cactttgaca tcaacaatgc cttattttgt aagtctttct
    29401 catcatttgc ataaaatatt ggacaacctc caccattttt catattctta tccaacgtaa
    29461 ctatagctag tgtatattca tttaaaccta tatccattcc catcttatgc acttcctttc
    29521 tttcctattt tataattttt tagtgataag ttttttcctt tagaacttga aagtattggg
    29581 catgacttta cggcttccat tactttttcc acattcttat ctactggtaa ataagcaatc
    29641 attatacttt catcctcttt attccttcta ggtaatggta cgaatgaagg ttctccataa
    29701 tctccatata aacctaacct tgaatatata ttgtatacta ttgcctgtct ttgacctgag
    29761 tcaaataata tccctgcatt agcataatct ttatttttag gaattatttg tattcctaca
    29821 cctctattaa tatatacttc tctatcactt tttagaccta tatttgttat tccctttaaa
    29881 tctcctacct tcaatataga ctcatctaca aaactaatct cagcaggcat tacatcagct
    29941 atgtctccaa tactttttct agtaagaagt ttttttaata taaaggctaa cactaatcca
    30001 caaattgaac ttattaccaa actcattcct tcacttattt tcgtttctgc aagtattaca
    30061 taatacaatc ctattgttaa aaaagatgta actatacaca tataatttct tacttcatag
    30121 gttctagcta tctcttctat gaatgcagct cctctcttta caagttgaac ttcctctaag
    30181 ttttgcaatg tatttcttct atttgctctc acttgtctaa attgctctgc tgctagggat
    30241 aaaaaagtta tagatgtata agaacgattt ataagagctg gaaccaataa tgctcctaat
    30301 gaagaagcga cgaatgctaa tataagttga gatagtaaaa catttggttg cgttggatac
    30361 tgtttttggt ctaaattcaa cattataatt ctagatgtta taccaataat aacagcaaca
    30421 aaaaatactc tatccattaa atcactccta aaatatttaa tttgaaattt tacatgtaaa
    30481 tctacaaagt ctttctagaa aaatcactct gctttccttt tgatgtttct atgataggtg
    30541 tacttttcac tacatctatc atgacatcta tatctttgag tattggcata tatggtataa
    30601 ccacagtact tttttctaaa ttggtcttag atatagcaac aatatcaacc tcatctacat
    30661 ctctatctat ccccatatga ataaatagat tatgtattat agcttgtctt tgccctatat
    30721 catttactat tccaaatgca cctaaatctc ttggtataac ttctatagcc agacattcat
    30781 ttctatactt ttctcttgta ctttctagac ctatgttagt tattattaca ccatttacca
    30841 ttaaaatagg tccttcaaaa cttatttttg caggaactat atctgctata tctgctatac
    30901 tgttccttct taaaaatctt ctaaaaatca aaccaacaat agctcctgac acaatagcta
    30961 agattgtgca ataaaaaaag ctaaggccat actttcttgc aaaaacgata taaactatcg
    31021 aagataccag tgcagaaaaa agacttatat aacttctaga ttcatacgta gatgctattt
    31081 cttcaacgta agcgtctcct tttggtacta attcttcatt atcgatattc tttagtgtta
    31141 ttctttcttg ttctgcaaga ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg
    31201 ctgcaaactc cttatctata agtgcaggta atgctatagc acctaacgaa gctgaaagcc
    31261 cagatataat tatctgttct aaataatctt gtggtcttga tgggtattgt ttgtctgtaa
    31321 ctcttaaaac taatcctcta cataatattc ctacaactaa tgcaactata aaagagtgtc
    31381 taaataaatt tgcattcaat aaactttctt ccattttctc agctccttct cttatatagt
    31441 ttgtgcaaaa aacaaaaaaa attcccttct atttaaggaa atttttttgt aatattgtaa
    31501 ctataaattc gctaccaatg ccaagttcac tttttacttt tatacatcca tttagtgatt
    31561 tgactatatg ctttgttata gccaatccta aacccgttcc tcctacatcc ctacttcttg
    31621 ctttatctac cctataaaat cgttcaaata tgcgttctac atcttctttt ggtatcccta
    31681 caccattgtc tataacttta attacgattt tttcttcatc ttttaacact acaacttgta
    31741 caatcccatt atcttgggta tactttatag cattatcaac taggtttaag aacacttgct
    31801 ttatatagtc tttattggat agtatactta ttgatttatc atcacattta tataaaactt
    31861 ctatattttt ttcactagct gaatgttgag ttatttcata tacctcttta aatacatcat
    31921 atactacaac attttccata agaagagtat ctttattttc tacaaatgat aacagcaata
    31981 tatcatcaat caaccttttt aatctatctg attcgctttc tattatttct aagaatctat
    32041 ttctagtatt cacatctata ttttcatttg actttagtgt ttcaacaaac ccacttatag
    32101 atgtaagtgg tgttttaagt tcatgactta catttgcaac aaaatcactt ctcatatttt
    32161 caagtttaac ttttttagta atatcttcta tatttactat cgaacctatt attacgttgt
    32221 tagtattctg aagataaact gggtctactt ttatcttata tacgatatca tctttagtag
    32281 taatattagc agtttcatta tctttagaac caataaattg aagtatttgt tttaatattt
    32341 cttcttcttt tattacaaca ttaatattat gaccttcaac taattgattt ggttcactct
    32401 ttatcatttc ttttgcttca tcatttatca gaagtatatt tccatttaca tctatagcta
    32461 aaattccatg agatatactc tttaatatag accttagctg taaatgttta tactgtactt
    32521 cttctaaggt tttatccata ttctttatca tatgattaaa atttctagct aattcgccaa
    32581 gttctccttt ggctgatatg ttaagcctag catgatattc attattactt actttttttg
    32641 agacgtatat aaactctttt aaatatttcc ttaatgttat tgtgtatctt acagctaata
    32701 taacaatggc tatggacaac ataacaataa aaaaatatat actatttaaa tcttccacaa
    32761 gataacactc ctaaaatata acctttttaa tctattttgt atcctacacc tcttatagtc
    32821 tctatatatt tttccgattt atcttcatct tctatttttt ttcttaaata tcttatatga
    32881 acatctactg ttctagtttc tccatagtat tcataccccc atattttatc caaaagataa
    32941 tttcttgaaa gtacttttcc tttattttga attaataatt ttaacaattc aaattcctta
    33001 agagttaact ctatcttttc agagcctttg gtaacttcat gttttgataa atctagtttt
    33061 aaattacctg tagtaagtat attgttttct tcacctaagc tagtcaaatt atatctcctt
    33121 aaaacagaac taatcctagc taacaactct tttatactaa atggcttagt tatatagtca
    33181 tcagcaccta tttcaagccc ttcaaccttg tcattttcca tatttttagc agtaagcatt
    33241 ataacaggaa tattttttaa gtccttatca cttcttattt ttctcaaaac ttctattcca
    33301 cttatatttg gtaacatcca atctaaaagt attaaatctg gtttaatttc cttagccttt
    33361 ataaatccgt caaatccatc atatgaataa ctaacttcgt aatttgacac ctccaaattg
    33421 aattttaata gctcaactat atgcatttca tcgtctataa caagcacttt agtattcatt
    33481 aaaaagctcc tctctatttt atctgtagta ccgttcctat tcgttctgat gccttattat
    33541 gttttctata agagtggaat ttatctgctc tacaactagt gcataaattt aaatttatta
    33601 tattttcttt tttaactcca ctacatctaa gcatatactc attaattttc cataaatcta
    33661 aatagtaact tccctctttt attatataga acttttcatc attatttgta agaattgtgt
    33721 taaatttttc tactaaatct tcagacactt cataacaaca tggtccaatt gatggtccaa
    33781 ttatacatac taaatcttca ggaactgttt tatagcattc agacattaat tttattgttt
    33841 tatgacctat attactaaaa gtccctctcc aacctgcatg agccagtcct atggctttat
    33901 ttttagcatc tataattgat ataggtacac agtctgctgt aaacagtaaa agtggtactt
    33961 tttccaagtt tgttataagt gcatctccat ctcttctttt ccctatagta tctttattga
    34021 ctatattaac tatatctgaa tgtatttgag aattactagt tagattttca aatgtcaaat
    34081 cacattcttt acatactttt aacatatctt ctctattttt tgcatctata ttcttacatg
    34141 ttataactag atttacagaa tttaattttt catcaagttc ttcacaatta ccacttaaat
    34201 taacatactc tttaatatct tgtacatttt ttatatttat ataatctttc atcatttgtc
    34261 tcctttttct atcagtatac ttttaagttc atttacaaaa gtatttatat ctttaaattg
    34321 tctatacaca gaagcaaatc ttacataggc aacctcatct aactctttca atttatccat
    34381 taccatctca ccgatttttt tggtttcaat ttcttctata aaacatttat ttatttcatt
    34441 ttcaatatgt acaacaatac tttcaatctc gtcaatagaa acaggtcttt tttcacatgc
    34501 ttttaacaat ccatatttta tcttttctct atcaaaatat tctctactat tatccttttt
    34561 aatgaccatt aaaggagttg tttctatttt ctcatacgta gtaaatctct ttttacatga
    34621 ttcacactca cgtcttcttc tgatagattt taaatctgta tgtctagaat ctattacctt
    34681 tgattcttta taattacaat aaggacattg catctatatt cctcctttgt ttaactatat
    34741 ctcaaactca tctaagctta attctgaacc tatatttact attattgtat cacaacctat
    34801 tttaagtata tcattccaaa atataacttc ttcatcccct cctcttgaaa aaaaacttct
    34861 acttccatct ccaaaaatgg taaatgagac taccttacct tcgtgaatat ccatgtctat
    34921 gtctattata aaccccatcc tttttccatt ttttacattt ataatttctt tttccattat
    34981 atcagaaact ctaatcatat tgtactcacc ctttctttaa aaataaatat gtatcgaagt
    35041 aaaaaaaaat gacctatata ggtcatttaa attaaaataa tgtttacagt ccatggatta
    35101 cttatacata ttttctcata ttctttaaag catttttttc aattcttgat acttgtgctt
    35161 gagatatacc gatttcatca gcaacttcta tctgagttct tcctttgtaa aaccttaaat
    35221 ctaataccaa cttttctcta ctatttaatt tcttaatagc ttctttaagt gatatttctt
    35281 gcaaccaatt ttcatcagta tcttttttat cttgaacctg atccataacg aatattgcat
    35341 ctccattgtc ttgatatact gggtcaaaca gagatattgg gtcttgtatg gcatcaagtg
    35401 ccattacaac tgattctact tctaattcta attcctttgc tatttctgat actgtaggtt
    35461 ctttagaatt tgttctaatt aatctttctc taacttgtag tgccttatat gctatatctt
    35521 ttaatgacct acttactcta attgggttat tgtctcttag ataccttcta atttctccta
    35581 taatcatagg aacagcataa gttgaaaacc ttacattttg gcttaaatca aagttatcta
    35641 tagcttttat aagaccaata caacctattt gaaataaatc gtctatattt tctcctctgt
    35701 tgttaaactt ttttattaca ctcaagacta atcttaaatt ccctcttaca aactgttgtc
    35761 tggcttcttc atcaccattt tttatttgta ataaaagttc cttcatttgc ttatttttaa
    35821 gaactggaag ttctgatgta ttaacaccac atatttcaac cttattaact tgcatatatt
    35881 ttcagtcctc tctcaaaagt attcatctat tgaaattatt tacatttcca atttttttat
    35941 actttttaag aagaattaat ttatcattat acaaattttt tcatttcttt ttgcaacctt
    36001 gagattattt tcttttcaag tctagatatg taggactgtg atattccaag cattccagca
    36061 acttcttttt gtgttttttc aataccttta tcaataagac caaatcttaa ttccataatt
    36121 tgcttttctc tgtcacttaa tctgtcaagt gccataacca acaaatctct atctatttct
    36181 tcttcaatta ttttgtatat ttcatcattt tctgtaccca atacatcaga taataaaagt
    36241 tcgtttccat ctaaatctat atttaaaggt tcatcaaatg atacttctgt tttcttttta
    36301 ttattttttc ttaagtacat taatatttca ttttctatac atctcgaagc ataagttgcc
    36361 aattttatat ttttattcag tttaaaagta ttaactgctt ttatcaaacc tatagttcct
    36421 attgaaatca aatcttctac atctatacct gtattttcaa attttctcga tatgtagact
    36481 actaatctaa gatttctttc tattaaaatt gactttacac tttcatccgt ttctagcttt
    36541 tgtaaaagct ccatttcttc ttctggtttc aatggaggag gaagtatatt tgcccctccc
    36601 atatagtata ttccctttgg ctttattatt tttaatttaa tacctaacat agtaataaag
    36661 cttattattc tttctttcaa tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat
    36721 ccaggcaata tatttggatt cattattgct ccatattctc tatcattaaa atttgaaata
    36781 ccaagtatta tatttcctat cttttgctca tctacttcta tatagtcagc ttttaagcct
    36841 attattatgc ttgtcttatt acttccagca tgtttataag gtattattct aactctacct
    36901 gaagttttat catctaaact tcctataatt tcttgtgctt tcttcatatc tatatcttca
    36961 taattaatat ttaatagttt ttctggaata aatccttcta acacactaga cttaactatt
    37021 attacatcac tttgacttat tgggtctttt aacaaattac cactatctac taaagcctta
    37081 cagcaaaaac ttttttctag taagtttata tttatagtct ttgtaagctc cttaatgtac
    37141 ttaagcattt tcaaatcttt ataaatgtat tttaataatt cacaactcac ataagtacat
    37201 gcaattaaga aagatatttt taagtgactt attcctgtaa aataaattat aaaataagtg
    37261 cttccagaaa taaaaatgtt tactagataa aaaactaacg ctacatgaac ataatctttt
    37321 ttgctcttac ttctaaatga aattaaagtt attatactca tgattattat tttaaatggt
    37381 acagtaaata gtatattcat tgatggatac aaataagcaa cagaataggc acctcctaaa
    37441 ctagccccca ataacttatt ttttttagga ttatatcttt tagtaagaat agaagtacaa
    37501 cttataataa tataatttat tactaggttt tctataacat agtactcaat atacaccatg
    37561 tatcccccct taaaaccttt aaacatatta taagatagta tgtacaaaat tttatgtcac
    37621 ttttgatggt cgaatccaaa acaaattcta tttaaaaaga acaatgatat atgtcttata
    37681 ctttatgtcg tcaaaagata tttttttaat accaaacaaa tagacttttt tgtagaatcc
    37741 aatatatcta ggtagaagtt attcgctcta cttttggtat atttacaaaa aaagactaca
    37801 taatttaata atatatcaag taaatctttt acttatataa aatcaaatta tatagtctgt
    37861 aaattcaaat ataaattatt gtgctagtaa tccatttgca tattttgcag attcatttag
    37921 tgcttgttct ggagttatgt ctccagctat catcatttga agttgtttcc ataaatattc
    37981 atatatctct actccatgag gtccaactgt caatggtcta tacatatgtt tatctcttgt
    38041 caccatttct tcaaatcttg ggtctccttg gtatttttca cctttagcaa ctggagctct
    38101 aggtatttct ttgtggaaag cttccattga ttctttgctt agcatatact caagaagttt
    38161 aaatccaagt tctttatttt tacatgctga cattaatgtt aaatcatcta ctgaagcaaa
    38221 acttccaact gtcttatctt ctaatgatgt tacatatccc cagtttaaat ccgggaatga
    38281 tttatcaaaa gtacttgtag cagcagagga tagcattata gtaaatgcag cttttcctgg
    38341 tccaaataca ctttcaatca tttcattact atcttttgat aaagcatctt ctggaatata
    38401 ctttttcata tcatttataa agtttgcagc tttcaagcct tctgcattat taaatttaac
    38461 tgattttaaa tcatcattat atatatctcc accagcttgc cataaatacg gataccagtt
    38521 ccagtttaag cttccaaata ctttagaacc ccaaccttgt gcaagacccc actggtcgat
    38581 ttttccatct ccatcagtat cttttgttgc tttttcacag attcttttaa aatcatccca
    38641 tgtttttgga ggtttttctc ctaatttttc tagtatatct ttattgtagt acaatacgcc
    38701 tggattagca gcttctatag gtaaaccata aataccaccc atcatttttg catcatctaa
    38761 gtatagataa ttatctgaag ttcctgattt ttctaaataa ggtgttaaat cttctaccgc
    38821 acccatttct ataaattgag gaaacatctc tgcatacatg tatcctatat ctggaccttc
    38881 acctgcactt attgctgttg catatttttc tgcgtagtta tcccatggta ctatctgaaa
    38941 ttcaatttca cagttatttt ctttttcaaa tttttcaaat atcggcttcc atgcatctgc
    39001 atcatggtct gttaatggtg cagaccatac aactaatttt tctttaccct ctttgccttt
    39061 agaatcttct ttttttgaac atcctgcaag aattgatact gatagaatcc ctaccataac
    39121 taaagaaaga acctttttta gtttaatcat gcttaatccc ctttcagact ttaatttgtc
    39181 tttttcctca aatgtttaca tttaattcag atttaatatt aacaaataag attactcttg
    39241 tctaatcata aaaaactata ctaactccta taactatatt ttttttctat acctattaga
    39301 tttaattcat actaaaaact tactttttaa attttatcaa tatttaaaaa tatttttaag
    39361 tctagccaca aactaactat ctaaattgaa caaaataaaa agaagtctat tgacttcttt
    39421 ataagcttac atattatctt cttcttctta ggaatgtagg tatctccatg ctctcatcat
    39481 catcttcttc aattatactt gcctttttag gaggctctat tttttcctca gcaattactt
    39541 cttcttcaat ttcttttaca gcttgtttta ctgttgtatt taatgaagaa cgtatgcttg
    39601 atttaggttt agtatctaaa tctaaggcac cattttgtaa tccttcaaat cctgtcgcga
    39661 ttactgtaat cattatttca tcacctaaat cttcttttat agaagctcca aatataacat
    39721 ttgcttctgg gtcacaggat tccataacta aagttgatgc ctcattaact tcaaataatc
    39781 ctaagttagg accacctgta acattaagaa gtactccttt agctccttgt attgatgttt
    39841 ctagaagtgg agattgaata gcttgtcttg cagcatcaat tgctctagtt tcaccactag
    39901 cactacctat acccatatga gcaagtccct tgtctttcat tatagtagta acatctgcaa
    39961 agtccaagtt tattaaccct tcaactgcta taaggtctga aattgattgt ataccttgtt
    40021 ttaatacatc atctgcaact gcaaaagcat ctaacattga tgtatttttt tgtactatct
    40081 gtaaaagtct atcatttggt atcgttataa gagtatctac ttttgattta agttctgcta
    40141 ttccaccttc agcgttcttc attcttatct taccttcaaa tgcaaaaggt ttggtaacta
    40201 cacctactgt aagtatcccc atttcttttg ccaagcctgc aaccactgga gcagctcctg
    40261 taccagtacc accgcccatt ccggctgtta caaagaccat atcagctcct tgaagtaact
    40321 ttactatttc atctttactc tcctcagcag ctctttttcc tacttctgga tttgctcctg
    40381 ctccaagtcc tctagttagt ttttctccta tttgaacttt atattcagct tttgatgtat
    40441 ataaagcttg tttatctgta tttactgata taaattcaac gccttttagt tgagcttcta
    40501 ccattctatt gacagcattg tttccaccgc cacctacacc tattacttta atttgtgcac
    40561 attcttctaa ttctacgtca aagtttagca tcataaaccc ccttctctca tgattcaaag
    40621 ttatcgtatg tattaattat atcattttct tcttttattt tgttaacctt taagtaacat
    40681 attccacaaa aacgtaaatt ttccttttta aatttcttac ttatattgtc aaatattact
    40741 ctttaagtat aaaataattt acgtatatat taaatgctct ttaattttta cgttttttat
    40801 tcataatatt actaataata tatcgtctaa ttatagataa gttattaaac aatctcatac
    40861 caaaaactaa tacagcaaca tagtaaagag aaactcctaa cttttctcct atataagtca
    40921 ataatatagc caatatagca ttacctatga aaccagtaat aaaaattaca ttatcatagt
    40981 tatcttcaaa attggatttt atcgctccaa aaatagaatc cattattgca agaatagcta
    41041 ctgacatata tatggaataa tccatagaat atgtaaatgg aatatagtaa cctactacta
    41101 caccaaatgc taatcctcct aaaacccata acataaatct tcacctcttt attttacttt
    41161 gaaacacaac tacattacaa tcctttttat taattttttt tactttttct ttattttttt
    41221 gtatttttcg ttttattttg caaataaatt cactttattt ccattatttt cctgaacttt
    41281 tatattaatt ccatatatat ccttaagaga tttagcatat gattctggag agtttatagc
    41341 tgcactcaat aaatctatat ctcctatagc ttttattata aatggttgtc catatgtagt
    41401 atcatttact gtaatagtag ctcctgaaca ttttatctta cttgttgcta gtactctttc
    41461 tccatttata gaaattgctc ttgctccagc cttttttaaa tcatttaaaa ctctcaaaat
    41521 atctatatca tgtataatca aatcgtttgg attctgtccg tcttttaatt ctctctcact
    41581 atccctcata gtaatagtta tgcctggccc tgtaatagtt gaataaccac ttaattcttt
    41641 aacttcttca agctcttttt gcataagtgt ttggatagat tcatttccat tcttttttgc
    41701 ttctttatat tcattcaaag tttttttgtt ctttttaaga gtttctttta agttttttat
    41761 ttcttttttt gtattttcta cctcactttc aatactttca gtttgaccta atgttatgta
    41821 tactctattt gggtctagtg attttatata agttgatatg aaaacaccta atatgaatgc
    41881 tcctaatata actattttct ttttcatact attatttccc ctctgctttg tttatatcac
    41941 tatgaccatt tatttctata ttgtcttctt tggtcacttt tactttggca ttataatatg
    42001 tttctatttc ccagacaata ccatacttta tattaagtgc cgaagccaat gtatcagaat
    42061 caccaattgc ttttattata attggttctt ttatgacagt gtcattcata taaagcttgt
    42121 cttcttttaa atacatatat gtattagaaa ctattctctg gtcgtttata gatatggcac
    42181 ttgcttgtgc tgaatttaac ttatttatca ttgacaataa taaatcataa ttatatagta
    42241 tactatcatt tttatcagag tttcctaagt cttttgtaga atttatctca atttttatgc
    42301 ctgttccttc aacatctgta tatccagcta atgtttcata tttttctatt tctgacttaa
    42361 gtatgtcttg ttctccttca ttatttttgc ctctataagt ctctatatct ttcttcacac
    42421 tattaacttc tgattgtaga ttctcttttt ctttttttaa agatttaagt tcttctgtaa
    42481 gttgctctcc tcttttaaaa gttgtcatcc ctctattttc tatattaata attttaagtt
    42541 gtaaactaac aagaacaccc aatacaatac ttcctatgat tatactttta tttataaatc
    42601 tttttttcaa tatatcactt ccttacaatt gtctatcctt ctggactata cactgccaac
    42661 tttccatagt taagatttat agtccctcca ttagtatttt tattttgcag gtcaatcaga
    42721 attttactta ccctagatat attataatct aagttatcat cattagaaag taagattttt
    42781 atattacttc ttgtaagcat ttctatattg ctttcttttt ttaagtttac ataatttatt
    42841 tttctataaa ttccttcttc ttttaaataa tttaaagttt taaatacatt ctccttagtt
    42901 ttataatttt taaattttat agatttatta tcatcaatgc tataatctac atctactatc
    42961 aagtcttttt tactttcatt agaacctctt agttcttcca ataaatttcc ttttttatct
    43021 atataacaat aattatcttc atcttttaat actgcaaata tttctttttc ttttaagctt
    43081 ataattatct tattgggaag ttttcttttt atttccacat tttctatata tggatttttt
    43141 ataagtttat ttttcatatc tttaaaattg tatgcaaaaa tattttcatt tagatttaca
    43201 tttagttctt tcattatatt tgatttggtc accctcttat ttccaattac atctattttc
    43261 tttacatcaa ataaatcact ttttacaatt atacatatac aaaaagataa cataagtata
    43321 aaaactaaaa ctatcatgac attattcgta tttaactttc ttcttttttt caaatataat
    43381 caaccccttt ggttaataaa tacaccttta gagcttgttt ttcctacaaa aaaattataa
    43441 tatttaaagt caaatttttg cattttatta cataacatcc aaataaggta ttaaatttta
    43501 taaataaatc aatatttcat caaaatagac atagcatact acatactatt tatatattta
    43561 aatctgtata catacaaata aacccctaat ttataataat ttagaaaatc taatatatta
    43621 taaattaggg gtaaatattt acttatcttt tctttattcc tattacctta gcttgacctt
    43681 ctatacttgg agtcgtttct ttttttattt cttttacttc cttaataact ttttcttttt
    43741 ttgttttttt agaagttgac ttttgtgttg aattgtatac tttcattatc tcatcatata
    43801 taaggtctat ggcttcaggc ttacctatag ttttacttgc atttgccata tcaaccaaaa
    43861 gttctctatc tcctaaaagt ttaaatactg ctgtatttag gctctctgga gttagatttt
    43921 tttctaaaat agcaattcct gccccttgtt tttctatact cttggcatta tactcttgat
    43981 gattttcagc agtataagct ttaggtatta ttatagatgg ctttccaaga gccgttatct
    44041 cagctaatga aattgctcct gcgctaccta taaccaaatc acttgctgca agtgcatttg
    44101 ccatatcttc taagtaaggc acaacttttt ggtatggttt aagatttata tcgctaatac
    44161 ttcccataaa ttcatcataa tagcttttgc ctgtagcaaa tataaaagct atatcttcat
    44221 ttaccatatt ctttattaca agtctcattg catcatttat ttttcttgaa cctccactac
    44281 caccatagca aagtaccatt cttttttcgt cacttattga tagattcttt cttgcaatat
    44341 tttttcttga taataaaatt tcttttctaa ctggatttcc tgtaaaaact aatttatctt
    44401 ctgctgcctc tggaaatctt ttatgcgaat cttcaaaact tgtaagtacc tttgtaactg
    44461 ttttagataa aatcttatta gttacaccag gaaaagaatt ttgctcatgt attatggctg
    44521 gtattttccc catagatgca ttaaataaaa caggtccact aacatatcca ccagttccta
    44581 taactatatc tggtttaaat ttcttaacaa tctttcttga ttgctccaaa cctttaaaaa
    44641 gcttaaaaac tctttttaca ttatcgaaat ctatttttcg cttaaaacct tgaactgtta
    44701 cagtctttaa ttcaaatcca tacttaggaa ctatttctga ctctattccc ttttccgtcc
    44761 caacaaaaat tatctcagcg tctggatgct catctcttat tttgtttgca atagctatag
    44821 ctggatacac gtgccctcca gttccaccgc ctgataataa aactttcatc aaatcatcat
    44881 ctcctgttaa ttaattttga catgctttga gatgtttaac actattccta cagctcccat
    44941 aaagatggtg agtgaagttc ctccataact tataaatggt agtgctacac ctgtagctgg
    45001 cattgatgat gtcgcaactg ctatatttaa agctgcttgt attccaattt gtgcacctat
    45061 acctataaca accatacaag caaatacatt agaagtcttc aaagctattc ttacacatct
    45121 gtagactaaa actacaaata gcataataac tattatacaa ccaatcaatc ctaactcttc
    45181 tcctattata gcaaatataa agtcattttg tggctctggt atataaaaat acttttgttt
    45241 actctttcct aagcctagac caaacaatcc acctgaacct agtgcatata gaccttgtat
    45301 tacctgatac cccttaccaa gagggtcttg aaaagggtct aaaaatgatg taacacgact
    45361 aagtctatag ggttcagcaa ttactaatgc agcaaataaa gcagccccac tccctatcat
    45421 agcaaataca attttcatat ccattccagc aacaaatatc attacaaaag taactagaat
    45481 tacagtacct gctgttgaca aatttggctg aagtattatc aacgcaaaaa atatccctgg
    45541 aactacgaga agtggtacaa caccttttgt caaagatttt attttatcat aatttttttc
    45601 aattaatttt gcagttaata ttattgtagc aaactttgcg atttctgcag gttgaaatgt
    45661 taaagctcct attcccaacc atctttttgc tccgtttgct tctatcccta aaggggtcaa
    45721 aactaacaat aataatacta ctgctatagc tccaattgct gtagcatttt tcttccaaaa
    45781 actataatct attcttgatg taataatcat aactataaat ccaagtacag catatattac
    45841 attctttttt agaaaatagt acgcatcatt gtgtttaaat gaagattgta taaaacttgc
    45901 actaaacacc ataactattc ctacaaatac taaaagcata gtagtgtaaa acactactcc
    45961 atcaaattca gtcttcatcc ttatgtcaat ttgttttttc aaattttctt taggcatcca
    46021 actaatctac ctccattctt ccatttaagt aagtttagag gtaagagatt attttaaatt
    46081 gtttacatta tctttgaagt cttttcctct tacttcaaaa ctttcataca tatcccaact
    46141 tgcacaagct ggtgaaagaa gcactatatc tccacttttt gaaatttcat aagaagtctt
    46201 aactgcttct tccatatctt ttactatatg tatgtcatta aatcctttat ttttagcaca
    46261 attttctata tttgatgcag tttctcctaa taagaccaat gatttaacat atgactttgc
    46321 agtttcaaga agttcatcaa aattacttcc cttatccatc ccccctgcta taagtattat
    46381 aggtctatca taagactgta tagcttttat agtggaatct ggattagttc cctttgaatc
    46441 atttacatat attacattgt ctaaatttct tacaaactct tgtctgtgtt ccactccttt
    46501 aaatgtcata agtacgtcct ttataacttc taaatcaatt ttgcagacat atgctattgc
    46561 tatagctgcc atacaatttt ctaagttatg acctccaggt aaacttacat catctttatt
    46621 tagaaatttg attttatcat ctatatctat tattatgtca ttattttcta aatatactcc
    46681 tccatttact tttttagttc ttgaaaagta aattactttg gcattgcatt tatcagctaa
    46741 agattttact atctcatcat catagtttaa tacacagaaa tcctcaacag tttggttcat
    46801 aaatatattt gcctttgctt ctatgtattt ttccatagta tggtgtctat ttaaatggtc
    46861 ctctgtaata tttaatattg cgcttacttt aggtctaaat tcatctatac tctcaagctg
    46921 aaaactgctt agttcagtta ctaacacaga ctcttcactt gaagtttcta tagtatctat
    46981 tacaggattt cctatattac caactacata tgtatctcgt tttgctcttg aaaaaatttc
    47041 tcctaccaaa ctagttgtag ttgtctttcc atttgtacct gtaatcccta taaaaattgg
    47101 attattcgat agtttaaaag ctaattctac ctcaccaatt atatacttgc ctgaattttt
    47161 taatttcaat atgaatggta ggtctagtgg tacacctgga gacactacaa ccatatctat
    47221 atcatctaca tcttctgggt gatgtcctaa tatgtatttt atgtcattta tagatttcaa
    47281 ttcatcaagt atatttctca atttattttc atcttttatg tcatttacga ttatacttgc
    47341 acccaattta tctaaatgtt taattgtaga tataccagtt tttgcaagtc ctactaataa
    47401 aacttttttt ccttttaagt ccatttttca ttccccctag ccctaaaata ctgctattat
    47461 acttatccaa gctaaaacaa ctgttataat ccaaaaaata aatacaactc ttgtttctgg
    47521 ccaaccacac tgttcaaagt ggtggtgtat tggagccatc ttaaagaatc tctttcttgt
    47581 tgccttaaaa tatccaactt gaatcaaaac tgatagagcc tccgcaaaat aaattcctcc
    47641 aattataggt attataagca ctgagttagt tagaactgaa aatgctacaa ctgcacctcc
    47701 taatgccatt gaacctgtgt cgcccataaa cactctagcc ggatatgaat taaatcctaa
    47761 gaaacctaaa catgctccaa cagttgctgc tgctaaaact gcgacttctg tatttccagc
    47821 aatagaactt gcaaatagca taaagaatac agaaactatt aatgttactc ctgatgctaa
    47881 tccatctaat ccatcagtca agtttacagc atttactatt gctacgatta taaacatcat
    47941 tattggtata tataatattc caacatttat cacaaaatct gtaaatggta tcataagttg
    48001 tgaagcactt gatgatgatg tatactgata aaaagcaaca taaaaagata atgcaacttg
    48061 aaggattatt ttttgatatg cttttagtcc aagtgatctt tttagcttta tttttataaa
    48121 atcatctaaa aagccaataa aaccaaaacc tgcaatacat ataagaccta ctgccatatc
    48181 atgactaact tttactcttg taagtccagt tattagaatt gcaacaatca ttataattcc
    48241 acccatagta ggtgttccgt ttttagctag atgtgtttgg ggtccatcat ctctaactgt
    48301 ctgaccaaat ttaaattttc taagcattgg tataaatatt ggtcctatta taattactat
    48361 taaaaacgca atcaatgcgg tgtacgtaag ctctgttatt cctaacatca taaaactcct
    48421 ctcccttgtc aattaactca ttagtttaag cttatttatt tagatattcg actatttttt
    48481 ctaatctcat gcctcttgag ccttttacta aaatagtgtc tcctgttttt actaattcat
    48541 ttaacttgtt aaacacatct tctctattct caaagtgata tatattggct gaattgaatc
    48601 ctaattgttt tgcttcttct ccaataaaca ctgaattttc acctatagtt attataatat
    48661 cagtattatt catagatgat tttccaacta atctatgacc atactctgaa atttcaccca
    48721 tttctagtat atctccaagt atcgctaccc ttctaccttt atatcttcca agtatcttta
    48781 aagcagcatc catagaatct ggactagcat tatatgaatc attaattatt gttaattttt
    48841 cattttttat tatgtctaat ctcatttttg tccctttaaa attttttaaa ccttttttta
    48901 tataatctaa agaaatattt agacacaaac caactaatat agcagacatt gcattatata
    48961 tattatgttc tcctattgtt ggtataaata tttcctcttt tttcccattt attacacata
    49021 taaatgttaa actatcctct tccatagtat aactttcaca ataaacatca ttgtttttat
    49081 taaagccaaa tgtttttaac ttatatgcat gttctttttc ttttaatgta gataaaaact
    49141 tatcatctcc atttacaatt aaagtatttg tttcatcaaa gtttgaagta atctccatct
    49201 ttgctttcaa aataccctct tgtgaaccta gtttttctac atgtgataat cctatattcg
    49261 atatgactgc aattttagga tttactatat ccactaaata ttttatttca ttgaatccag
    49321 acatacccat ctctattaca gcacactcat gttctttatt tagattaaat aaagtcaatg
    49381 gaactccaaa atgattattt aagttttttt cattttttag tatatttaac tttgcagata
    49441 tggcagagta aatcatatct ctagtagtag tttttcccac actacctgta acacctataa
    49501 atggaatatc aaatttttct ttatagtatt ttgaaatatc tcctagagcc aaagatgtat
    49561 cttttacctc tattaaattt atatccgaac tttctaattt tattccatta ctttcatttt
    49621 ttataaaagt tttacatcca ctatcatatg cagacttcat aaatgtatgc ccatctaaat
    49681 tttctccaac tatggctaca aatgcatttt gggcatttgc ctccctgcta tctataacta
    49741 tatcacttac acagtcatta taatctccat atattaactt cccattagtt gcaagtacta
    49801 actcttttat tgttaagcac tccattatta aactcctcct ttaaaacctt agctatattc
    49861 tcttagcaac aaaaggcaat gtataccgtt atatatacat tgcctacgat aactcttaac
    49921 tttataatta tatactatat ttctgttttt ttgaagatgt ttatgggtat atgttaatta
    49981 tctgttactt tttagttttc aaaatatagg gttatacttg agtcttcatt aacttttact
    50041 ccaggttttg ggaatatatc ctttaccttt gtatcttctg gaagttctat atctgcatct
    50101 aagtctggtt ttaacttcgc atcctccaat gcctttacag catctcctat ttttagattt
    50161 cttacatctg gcactttaac ttgcttcttt tcatactcag ctttttcttc ttctttatat
    50221 actggtttaa ctcccaaata ttttaaagag tcattcatta tttctttcac tattggtcct
    50281 gctgtagtac taccaaatgc acttactcct gttggttcgt caacaatagc aagaactact
    50341 atttgtgggt catcacaagg tgcgattcca acaaatgaac atatatactt tcctggagca
    50401 tacttaccat ctataacttt ttgagctgta cctgttttac caccaagtct ataacctggt
    50461 atatacgcta tctttcctcc accttcagtt acaactgatt ctgcaatttc taacattttt
    50521 ttagatgttt ctttagatat aacacttctt actttttttg gttttactgt ttcagttata
    50581 tttcctttat tgtcagtata agattttacc actcttggtt gcattaaatc tccaccatta
    50641 gctattgatg atatagctgt tataagttgt attggagtta ctgatataga ttgaccaaat
    50701 gatattgtag ccaattcaac tggtcctaca tttttttcgt tatacagtat tcccttagct
    50761 tctcctggta aatctatacc tgtcttatcc ataagaccaa atgattctat ataatcatac
    50821 atttttccaa cacctaatct gcttcctaac tcaacaaata ctgggttgca agagttttgt
    50881 actgcttgtt taaattcttg tgttccatga ggtctgtaat gtctccaaca tttaatcttt
    50941 cttcctccaa cagttacact acctgtacat gtgaattttt ctccatcttt aattactcct
    51001 tcttcaagag cactggaaga tgtaataagc ttaaatgtag aacctggttc atatgtatca
    51061 cttactgctg gatttctcca catttgataa tagcctttta ttttatcttt atcattatat
    51121 ttttcaagtt cttcttgata atatggatat attggcgttc ttgaatcatt agggtcataa
    51181 tctggttttg atgctaaagc aagtatatca cctgttttag ggttcatagc tattgcagta
    51241 actttttttg cattatttaa ctcataagct ttctgtactg ctttttcagt atagtgttga
    51301 attacttcat ctatagacaa cacaagacca ttaccttgta caggctcata atatttttcc
    51361 attccttgtg gtatttctct tcctgaagca tcagtactta ctataagttt tccaggcttt
    51421 ccttttaact ttttatcata ttgcatttct acaccagata tacctgttgc atctgaagaa
    51481 gtatgtccta acacatatgg tgcaaaattc ccatatgggt aatatctttg attatcttct
    51541 gccacccata ttccacttaa tttagcatct cttatttgac ttgccttatc atcatctatc
    51601 catcttttta cttttacaag agccatattt tttttgctta ttaaagcgta aatatcttta
    51661 taatcttcat ccaaaatctc agctaccttt tcggctgctt cttttttatc ttctacttcc
    51721 acaggtttac accaaacagt atacttagtt acacttactg ctaactcttt catatttcta
    51781 tcatatatag ttcctctttt gggttctatt ggtatatctc ttgtttgttg ttctagtgct
    51841 tttgtactca accaatttcc cttcataagt tgtagatacc cagtcctaat aacaaggcaa
    51901 aaaaacaatg cacatgctag aataagaacg agtaccagcc ttttcttact tatcctcttt
    51961 acttttctca aatctattcc ccctagttat ccacatctat atactttatt tgtccatctt
    52021 ttggatagtc catattgagc aattccttag cctttagctc aatttctttt attgatgttt
    52081 tttcagtttc ttttgccttt agttcctcta atactatctc tttttttctt aaatcttttt
    52141 gtaaataata gatatcgtac ttcatttccg agatttttga ataaccacat agattcaata
    52201 ttattactga aaaagttgca atcagtaaaa tgcatttagc tcctattttt ttcttatttc
    52261 tctttttata attattcttt tttttatttt tatattcatt tagattatgt attttataat
    52321 tttttttcaa attctcaccc cgatagtata tagtttaaat tttcctctat actatatttg
    52381 ttattaatca tatttcttcc tctaaaattt catcgctaaa ttaatacact attacacaat
    52441 ctaattgtat ttttttctat acacaacaaa aacctataaa ttcacttata cttttatatg
    52501 aatctatagg ttagatatta atttctattt atttttatca cttctagtat ttttagataa
    52561 tttcttccat aaatataata tgtaactaac tatctactct ctgattccta agttctccat
    52621 tatattactt aataccatct atatctatat ttttatatct aaattttcat tatatatctc
    52681 aataacaaag aacatttctt atagtgttaa ttgtttttta attgctttac cagtgcaaca
    52741 aaatatataa tactttttaa ttaattataa taatcttatt ttatatataa gattattctt
    52801 tagccttata actacttata ttttttctgc tactcttagc ttagcacttc ttgaacgtgg
    52861 atttacttct atttcttcct cactaggtaa tataggtttt ctagttatta ttttcacctc
    52921 agattcttta tcacattggc atataggtaa atgctgtgga catatacaat ctgatgctaa
    52981 atgcttaaat gcatttttca ctattctatc ttctaatgaa tggaatgtta ttatacatat
    53041 tcttccacct tcattcatta tagaagaagc atcatttatc atctttgtaa ttactccaag
    53101 ttcattattt acttctattc gtattgcctg aaaagttctt ttagctgggt gaggtccatc
    53161 tattctagct ttcttaggga tagctttttt aattacatct actaattctc ctgtagtttc
    53221 tattggtttg tttgctcttt cttcaactat aaatttagct atacgcttag cccagttatc
    53281 ttctccataa tcttttatta tcttagttaa ttcatcttct gtataagtat ttacaacatc
    53341 atatgcagaa aactcacacc taacatccat tctcatatca agtggtgcat cttgcatata
    53401 agaaaatcct ctatcagctt catcaagttg atgagatgaa actcctaaat cagctaatac
    53461 accatcaatt ttgtaaactc ctattttttc taattcctct tttatatttt caaaattact
    53521 gtgaacaaac ttcactctac tttcatattc actcaatctc tcttttgctg tgcttatagc
    53581 atttttatct tggtcaaacc ctataaataa tcccttatct gacagttttt ttactatttc
    53641 tttagaatgt cctgctcctc ccatagtaca gtcaacatac acgccatctg gctttatatt
    53701 taaattctct atacactcat ttaaaagaac tgatacatga tgaaattcca tagttacctc
    53761 ccttaaattc actttttaaa ctattcttca ctaatattat ttttattttt ctttgataaa
    53821 taaacatgtg caattatgcc accaataact ccaattagac ttataacttg tgccattcta
    53881 agtggtccta acattaaact atctgttctt aatccctcta taaaaaatct tcctattgaa
    53941 tataaagtta tataagtaac tataacttga ccttcatatt ttttattttt tctaaatagt
    54001 aataaaacta taaatattcc aaaatcccat atagactcat ataaaaaagt tggatgtacc
    54061 tttactccat caaccattat tccccaaggt aaattggtag cacctccatg tgcttctcca
    54121 ttaataaaat ttccccatct tccaatagct tgtgctaaag gcattccaag tataactgta
    54181 tcagccattt ttaagaagtt aatattttta atcttagtgt atatataacc agctaatatt
    54241 ccaccaatta accctccatg tatagcaagt ccaccacctc taaaattaaa aatttgagac
    54301 atattttgag catagtaact ccaattaaat acaacataat agattcttgc acagattagt
    54361 ccaactggaa ttgctattat agctatattt aatacatcat cttctttaat tcccactctt
    54421 tttgcttctt ttattgctat taaagttcct aaaatcattc cacatgccat aagtattcca
    54481 taccacatga tgtctatgcc aaacagtgta aatgctactc tatccataac gtcacctctt
    54541 tgtattaatt gaaaatattt ccaaagcaat atgctataat tacaattaat tgtaattata
    54601 ctaaattatg ataacacatt aatgttaatt ctacaaaaaa tattaaaatt tattctacaa
    54661 ctccctacac tatattatat ctaattacta taaaattttt taaactgaat ctctcatgac
    54721 taaagttaca agtgtacata gatatttttt aatcaaaaag attgatgcat atatacatca
    54781 acctcttcta attcttatct atagctctta aatttttacc tcaatttata taaatatatt
    54841 ttataaattt gttttaccaa aataaacatt taaaaacata aactaatact taattagtta
    54901 ttcttattat gattaagttt ctatttaggc tctacaatag aaataacaca atactcttct
    54961 ttaaaatgct cttttaatct ttcttcaact tcctcaaaag taatatcttt aataacatct
    55021 aaatactcca atagatttat atccttaaaa acataagata taaaactatt acctataaag
    55081 tttatagaat caaagcactt aataaatgac cctatttttt tctttttagt tctctcaaac
    55141 tcttcttttg ataatccttc tttttttgat ttttctatat aatcaagaat tatttctttt
    55201 acttttttag gctctttaga atctccagct attatgctaa aagcataatc cacttgagac
    55261 gaaaatccag ctccaaaatt ttcatttatt aaaccttgca tatacaaatc ttcataaagc
    55321 ttacttcctt ttttaaataa catccctaca agtatatcag ttacaatctc tttacgtaaa
    55381 agttctttcc cttttaaacc aacattacta tctttaaaac ctatgttgaa cataggcata
    55441 gatataggga atttttctat aacctctttt tcttttacac cttctggttc ttctggataa
    55501 aatctttcta tactcttaga gagtctgtct actttgtaat tgtttgactt tttagttaca
    55561 tctataactt tttctacatc taagtcacca acaacaaata aagccatatt tcctggatta
    55621 tagaaagtat tataacattt atatagctct tccttagtta tcttatatat actatcaaca
    55681 gtacctgcta tatcaattct agcaggataa tttacataca ttgccttcaa gcaattaaag
    55741 tatacattcc aatcagggtc atcattgtac atttttatct cttgtgctat tatacctttt
    55801 tccttttcaa cattttcatc tgtaaaataa ggtgtttgaa catagtcaat tagatgttct
    55861 aaactctcat aaaaattttc agtagcagaa aataagtagg ctgtcattgt aaaattagta
    55921 aatgcatttg catttactcc taattttgaa aatttatcaa atgcatctcc tccatcaggt
    55981 tgctcaaaca tcttatgctc tagaaaatgt gcaatacctt cattcactct aattttctta
    56041 tcttctccaa ttggtacaaa ttctaagtca tttgaaccat aattagtagc taaaatagca
    56101 tactttttca taaaacccct tttaggcata aaatatacat ctaatccatt ttgcaatttc
    56161 tcataataaa cttcttcttt caatatatca ttaactattt tttccataga catctcctcc
    56221 attaatttct caagaagtat acagtatcaa gctctatatt ttttatagct tcaactatat
    56281 ctttttttgt aaccttttct atactagata ttatgtcttg tacttcagaa tttgtctttg
    56341 ccatagattg agagaaaaca aaatcagaca taccacctat attatcagtt atagatttca
    56401 tagaattaac taaagcaagt ttactgtttt ctaattcttc atcagatata tcacctgctt
    56461 ttacactttc tacttgtttt tttatcaatt ctacagcttt atcataatct ttagtttcta
    56521 taccagatga tataaacatg atacttttat acttctctat tgaagagaat atatagtaac
    56581 ataaactttc tttttctctt acattaacaa ataatttaga gtgtggacca ccacctaaga
    56641 cattgctccc aactactaaa gcataatatt tatctatgtc tgcataatcc acatttgctc
    56701 tataacccat aactaactta ccttgagtta tttccatttg ttcatctata acttttacct
    56761 catccacatt ttttataaaa tctgctcttg gtatatttat aatttcttct ctttcaaatt
    56821 ttagattttt acttattata tctacaactt tatcttcatc aaaattacct tctacaacta
    56881 tatctatagg tgatgtttta agtatgtttt tataatgttc atataactct cttgaagtta
    56941 tactatctat ttcatcttca taaccaaatt cacttacact atatctctct cctttacaca
    57001 tagcctcaat acatttatct tgcgcatatt cctttttatc atttattatt gattgtatct
    57061 tttctcttaa attctctttt tcaatattta aatattcttc tttaaaccca ccatctacaa
    57121 caagcgtatt atttattact tcattaaaaa attctactac ttcttcaaat atactctcat
    57181 ctaaatactt ttcacttatg ttaattactt taaaactcaa tacttgacgt tctccacgtt
    57241 ttacagcatc agctcccata gatgaaccat ataaatcatc taatttattg gatattgctc
    57301 tagcactagg atatttagcg cttccacttg ctattattga tggtattaga gcattttttg
    57361 tagtttcttt ttcatccaat agtctctgaa catataaact tataagattc gatttaaact
    57421 tttctgtttt tatcaaagta agattaatat tattacctaa atttattatt tttttagtat
    57481 taaccatatg tacctcctat ttttatttaa acatcaattt taaaattgaa atttatttat
    57541 tttttaatta catatactca catataaaaa ccttctattt aattatatcc tttttattat
    57601 tttctaaaag caatattttt atttaaatta aatacaaata ttatttaaat caatatgctt
    57661 taaagtctag tttacaagct tgatatatat gatgtataat caatatgtat gtctataatt
    57721 aaaacaattt tgcaatccta tagattgatt gtttaaagtt atataaagaa tataaaaaag
    57781 aataagagag aggacgatta atatgaaatt aggcatagtt ggtttaccaa atgtaggtaa
    57841 aagcacacta tttaacgcaa taactcaagc aggtgcagaa tctgcaaatt accctttttg
    57901 tacaatagac ccaaatgtgg gagttgtatc tgttccagat gaaagattaa acaaactaca
    57961 agaattatat aattctgaaa aaatagttcc tactgctata gaattttgtg atatagcagg
    58021 tttagttaga ggagcttcta aaggagaagg tttaggaaat aaattcttat cacatataag
    58081 agaagttgat gcaatagttc atgttgttag atgttttgag gatgaaaatg ttgtacatgt
    58141 agatggttct gtagacccac ttcgtgatat agaaactata aacttagaat taattttctc
    58201 agatatagag attcttgaaa gaagaattaa taaaactcaa aaagctgcta aggctgataa
    58261 aactttaggt tctgaacttg atttattaaa atcaataatg tctactttag aagaaagtaa
    58321 gtgtgtaaga actatggaat ttacagaaga tgaacaaaca ttcgtaaatt cattggattt
    58381 acttacatct aagcctgtaa tatatgcttc aaatgtatca gaagatgatt tagctgataa
    58441 tggagaaaat aataaatatg ttcaacaagt taaagcattt gcagaaacag aagatgccga
    58501 agttgttgta gtatgtgcac aaatagaagc agaaatttct gaacttgatt ctgctgaaga
    58561 aaagaaagag tttttagaaa cattaggtct tgaacaatca gggcttgata agcttataaa
    58621 atcttcttat gctttacttg gtttaatttc attcttaact gctggtccaa aagaagttag
    58681 agcatggact ataaaagttg gaagtaaagc tcctcaggca ggaggaaaaa tacattctga
    58741 tatagaaaga ggatttatca gagcagaaac aatagccttt aatgatttgg ttgagcatgg
    58801 tactatggct gctgctaaag aaaaagggtt ggttaaatta gaaggtaaag aatacatagt
    58861 taaagatgga gatgtaatat tatttagatt taatgtttaa aatgattaat cgtatacact
    58921 ttgactatta tatat
//
LOCUS       DAENCV010000008        54266 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAENCV010000008 DAENCV010000000
VERSION     DAENCV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.008"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(317..2275)
                     /locus_tag="KQD82_000442"
     CDS             complement(317..2275)
                     /locus_tag="KQD82_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HBG4378946.1"
                     /translation="MKRAYYSSSVNEFIVKDIFTIFGEITCNDQFAADDLQKNTWKKE
                     IGILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPRHAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQKDQRIRMMKENILETYCCNEFNIGKYI
                     KKICTTYNRSSFNSDTWINSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKTHNRKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGIRKSEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFESLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSENVSTFVKAVLDVDKVAAKELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQNKIDATNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNNHFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFIIFVPI
                     GDESDITMQPNFYNGVYEYLKEIGIEEL"
     gene            complement(2468..3658)
                     /locus_tag="KQD82_000443"
     CDS             complement(2468..3658)
                     /locus_tag="KQD82_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HBG4378947.1"
                     /translation="MDKNKYKIETLMNHVGENLDGDYGAVNPPIYQSSIFARSTYEDI
                     EAYFEGSKDGYLYSRTKNPTNDILEAKLSALANAEASITFGSGMGAISSAILHFVKNG
                     DHIIASNNLYNPTMNFITKELKEKGNIENTIVEGDRIEDFENAIKQNTTLIYLECPST
                     SVFKMQDIKAIVALAKTHNIKTVIDNTWATPLYQRCIEMGIDLEVHSLSKYLNGHSDV
                     VGGCIIGSKKDIEEIRGSELALYGAKLAPFEAFLVIRGLRTLHARLSVHTKNAKEVVE
                     FLDNHSAVTKVYHPLSKYFNQKELAKKQLLNYTALLSFEIDAKDVNDVKKFVNKLDLF
                     KIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLINDLNDA
                     LSIL"
     gene            complement(3758..5224)
                     /locus_tag="KQD82_000444"
     CDS             complement(3758..5224)
                     /locus_tag="KQD82_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG4378948.1"
                     /translation="MKRKVTIQKQAISIVLFMAVLFCGLLLPMINNKPEYSLPIESIM
                     FISASIISLFIVVTTNKTWEEVFESGLDGVKGALPGLIILLVIGPLIAGFIMSGLMPM
                     LVYYGVKLINPRFVYATALILSALFSSFTGTSWGSAATIGVVMMGVGNAIGANNAIIA
                     GAIVGGAYFGDKMSPLSDTTNIAAIASKVNLYDHVASMLWTTGPATIIALVIYIACGF
                     IFPATNTNLNSPEITRLLADLSGMFNFNIILFIPLLIVLVGSAMKKPAIPVMTIAAFL
                     SLAFAVIFQGFSLADVLIGLNSGFSLDMVTWHEYAKPIEGQAYILGFFQKGGFWELGK
                     LLPISTSILFVVGVLGSIDAMPATVNVIFGWVKSRTAIICSSLLTGIAMIAMTANGIA
                     CSFVTAGIFGDKYDENNIDRRVLSRTTEDTGTLLEVLFPWTPAAIFFTQTLGVNVGEY
                     ALWSIVNWGTPIIAVILAVTGIGTYKNSKDKKQSNKVS"
     gene            complement(5501..6316)
                     /gene="rhaD"
                     /locus_tag="KQD82_000445"
     CDS             complement(5501..6316)
                     /gene="rhaD"
                     /locus_tag="KQD82_000445"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBG4378949.1"
                     /translation="MSIMEMNFIKDFMKITYDAWLKGWHERNGGNITYRLTPEEISGI
                     KEFITEEIEYIPIGVTVENLANEYFLVTGSGKFMRNISINTVQNVGIVKIDEKGENYA
                     IVWGLDKAKPTSEFPSHLMSHSIKKDKTNGLHRVIMHSHTTNLIALTFILPLEGKVFT
                     RELWEMATECPAVFPEGIGIVPWMVPGGIEIAKATQNLMEKHNIVIWAHHGTFCSGET
                     LDLTFGLMDTVEKSAEILVKVISMGGKKQTITGDNFRDLSKNFKVSISEDYLY"
     gene            complement(6327..7583)
                     /gene="rhaA"
                     /locus_tag="KQD82_000446"
     CDS             complement(6327..7583)
                     /gene="rhaA"
                     /locus_tag="KQD82_000446"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBG4378950.1"
                     /translation="MELKQRYEVAKKEYEKWGINVEEVLRVLSKVKISIHCWQGDDVT
                     GFEGNQQRLSGGIASTGNYPGKARNPQELRRDLDKALSLIPGKHKVNLHAIYAETGGS
                     FVDRDELKPEYFENWVKWAKERGLGLDFNPTIFSHPKSSDGLTLSHPDKKIRDFWIKH
                     CIASRKIGEYFGKELGQTCLTNIWIPDGYKDIPSDRLGPRRRLKESLEEILKVEINRE
                     YNLDCVESKVFGLGVESYTVGSNEFYLNFASKNNIISLLDTGHYHPTELVSDKLSSML
                     LFSDKVALHVSRPVRWDSDHVVILDDELKEIAKEIVRNDALDKVIIGLDFFDASINRI
                     AAWVIGTRNMIKALLSAMLMPHEELRKLQDEGDFTKRLAIMEEFKTYPMGDIWNYYCK
                     INNVAVKEDWIEVVKEYELTELLNRK"
     gene            complement(7604..8998)
                     /gene="rhaB"
                     /locus_tag="KQD82_000447"
     CDS             complement(7604..8998)
                     /gene="rhaB"
                     /locus_tag="KQD82_000447"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBG4378951.1"
                     /translation="MRYYLAIDIGASSGRHIISYINNGKLEIEEVYRFKNNLIKKDGH
                     FCWDISKLFSEIKNGIKKCIKINKIPVSVAIDTWAVDFVLLDEKDNILGNCVSYRDNR
                     TDGIMEEAFKIIDKKELYLKTGIQFQKFNTIYQLLAIKNQQPDLLEKAKSFLMIPDYF
                     NFLLTGKKVTEYTNATTTQLVNTTTNNWDEEIINKLGFDKMLFAKIKTPKTILGSLKK
                     ELVEELGCDLEVILPVTHDTGSAFMSTPVKNRSILLSSGTWSLLGVELERPKCKLEAL
                     DYNFTNEGGYEYRYRFLKNIMGLWMIQEARKCYDSKHSFTELVEHARLSEGFKSIVDV
                     NDDRFLNPENMVQEIIKYCIETNQTEPQTVGEVAYCIFNSLAKSYSKSINEIEKIIEE
                     KLETISVFGGGCQNELLNELIAKESGKKVLAGPVEATALGNIVCQLIAKNELNDLEQA
                     RDIIRNSFDIIQYN"
     gene            complement(9205..10176)
                     /locus_tag="KQD82_000448"
     CDS             complement(9205..10176)
                     /locus_tag="KQD82_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4378952.1"
                     /translation="MRQYIHDKLREITEEEKNILEGNYIIDKSIYTDNSQFIIDSNKL
                     LNIDELIHIRKHTRFAQFPKHKHNYIEFNYVYRGKLVQTIDEYKINLKQGELIFLNQH
                     VIHEIEASDEEDIIINFIIKPEFFDYIISLLENDNKIMNFLMTTLYTNYNLGEYIYFK
                     VSEKEEIQDIIEKIIIELYEPSVVNKVKIKLLVGLLLVELVKNSEDVENHAVDNYEKM
                     MIIEILKYIDKDYKKGSLSEIASNLNQGDYKISKLVKKHLGYTFKDLVQEKRLSKACE
                     LLVSTNLSIAEIIENVGYENLTYFYKIFKNKYGITPKEYKKNIKNSQ"
     gene            complement(10300..10581)
                     /locus_tag="KQD82_000449"
     CDS             complement(10300..10581)
                     /locus_tag="KQD82_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378953.1"
                     /translation="MKDETLEDILKQKTQAERYILEYIVTKNVKKLDNKKIVSILHTI
                     LCWEEINGTIVLTDKNILSLDEDIIISKHRRFIDIPKHKHNFIEFHLMS"
     gene            10767..11087
                     /gene="rhaM"
                     /locus_tag="KQD82_000450"
     CDS             10767..11087
                     /gene="rhaM"
                     /locus_tag="KQD82_000450"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBG4378954.1"
                     /translation="MNTIRKCFKMKLFKGFENEYKKRHDELWSEMIDMIHEYGGSNYS
                     IFLDEETNYLYGYIELKDAKLWDKSSETAICKKWWNYMSDIMETNEDNSPVSIELKSM
                     FYLK"
     gene            complement(11751..11891)
                     /locus_tag="KQD82_000451"
     CDS             complement(11751..11891)
                     /locus_tag="KQD82_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378955.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(12424..13311)
                     /locus_tag="KQD82_000452"
     CDS             complement(12424..13311)
                     /locus_tag="KQD82_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG4378956.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIK"
     gene            complement(13482..13685)
                     /locus_tag="KQD82_000453"
     CDS             complement(13482..13685)
                     /locus_tag="KQD82_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG4378957.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(13772..14806)
                     /locus_tag="KQD82_000454"
     CDS             complement(13772..14806)
                     /locus_tag="KQD82_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG4378958.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGIAAVVAGV
                     VYKKKKRKKI"
     gene            complement(15317..16678)
                     /gene="rlmD"
                     /locus_tag="KQD82_000455"
     CDS             complement(15317..16678)
                     /gene="rlmD"
                     /locus_tag="KQD82_000455"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG4378959.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(16705..17427)
                     /locus_tag="KQD82_000456"
     CDS             complement(16705..17427)
                     /locus_tag="KQD82_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG4378960.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPAGYISATTDKYDPTVLDLIDLSYLVFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(17427..17756)
                     /locus_tag="KQD82_000457"
     CDS             complement(17427..17756)
                     /locus_tag="KQD82_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG4378961.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            18086..19012
                     /gene="fba"
                     /locus_tag="KQD82_000458"
     CDS             18086..19012
                     /gene="fba"
                     /locus_tag="KQD82_000458"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG4378962.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(19147..20778)
                     /locus_tag="KQD82_000459"
     CDS             complement(19147..20778)
                     /locus_tag="KQD82_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4378963.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(21261..22298)
                     /locus_tag="KQD82_000460"
     CDS             complement(21261..22298)
                     /locus_tag="KQD82_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG4378964.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(22317..23102)
                     /locus_tag="KQD82_000461"
     CDS             complement(22317..23102)
                     /locus_tag="KQD82_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG4378965.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(23135..24268)
                     /locus_tag="KQD82_000462"
     CDS             complement(23135..24268)
                     /locus_tag="KQD82_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG4378966.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(24316..25443)
                     /gene="hadC"
                     /locus_tag="KQD82_000463"
     CDS             complement(24316..25443)
                     /gene="hadC"
                     /locus_tag="KQD82_000463"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG4378967.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(25443..26669)
                     /gene="hadB"
                     /locus_tag="KQD82_000464"
     CDS             complement(25443..26669)
                     /gene="hadB"
                     /locus_tag="KQD82_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG4378968.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(26684..27484)
                     /gene="hadI"
                     /locus_tag="KQD82_000465"
     CDS             complement(26684..27484)
                     /gene="hadI"
                     /locus_tag="KQD82_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG4378969.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(27523..28722)
                     /gene="hadA"
                     /locus_tag="KQD82_000466"
     CDS             complement(27523..28722)
                     /gene="hadA"
                     /locus_tag="KQD82_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG4378970.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            29399..30397
                     /locus_tag="KQD82_000467"
     CDS             29399..30397
                     /locus_tag="KQD82_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG4378971.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            30759..31286
                     /locus_tag="KQD82_000468"
     CDS             30759..31286
                     /locus_tag="KQD82_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG4378972.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            31313..31909
                     /locus_tag="KQD82_000469"
     CDS             31313..31909
                     /locus_tag="KQD82_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG4378973.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(31988..33571)
                     /gene="asnB"
                     /locus_tag="KQD82_000470"
     CDS             complement(31988..33571)
                     /gene="asnB"
                     /locus_tag="KQD82_000470"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG4378974.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(34173..35009)
                     /locus_tag="KQD82_000471"
     CDS             complement(34173..35009)
                     /locus_tag="KQD82_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4378975.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(35033..36499)
                     /locus_tag="KQD82_000472"
     CDS             complement(35033..36499)
                     /locus_tag="KQD82_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG4378976.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICEIYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(36502..38364)
                     /locus_tag="KQD82_000473"
     CDS             complement(36502..38364)
                     /locus_tag="KQD82_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4378977.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(38611..39474)
                     /locus_tag="KQD82_000474"
     CDS             complement(38611..39474)
                     /locus_tag="KQD82_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG4378978.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(39528..40460)
                     /locus_tag="KQD82_000475"
     CDS             complement(39528..40460)
                     /locus_tag="KQD82_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG4378979.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(40546..41097)
                     /locus_tag="KQD82_000476"
     CDS             complement(40546..41097)
                     /locus_tag="KQD82_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4378980.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(41326..41817)
                     /locus_tag="KQD82_000477"
     CDS             complement(41326..41817)
                     /locus_tag="KQD82_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378981.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(41845..42405)
                     /locus_tag="KQD82_000478"
     CDS             complement(41845..42405)
                     /locus_tag="KQD82_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG4378982.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(42557..43714)
                     /locus_tag="KQD82_000479"
     CDS             complement(42557..43714)
                     /locus_tag="KQD82_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG4378983.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(43751..44692)
                     /locus_tag="KQD82_000480"
     CDS             complement(43751..44692)
                     /locus_tag="KQD82_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378984.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(44779..45471)
                     /locus_tag="KQD82_000481"
     CDS             complement(44779..45471)
                     /locus_tag="KQD82_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4378985.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVEEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(45580..46392)
                     /locus_tag="KQD82_000482"
     CDS             complement(45580..46392)
                     /locus_tag="KQD82_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG4378986.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(46443..47354)
                     /locus_tag="KQD82_000483"
     CDS             complement(46443..47354)
                     /locus_tag="KQD82_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4378987.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEIFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(47413..48144)
                     /locus_tag="KQD82_000484"
     CDS             complement(47413..48144)
                     /locus_tag="KQD82_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG4378988.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            48233..48484
                     /locus_tag="KQD82_000485"
     CDS             48233..48484
                     /locus_tag="KQD82_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378989.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            48717..49136
                     /locus_tag="KQD82_000486"
     CDS             48717..49136
                     /locus_tag="KQD82_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4378990.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            49161..54218
                     /locus_tag="KQD82_000487"
     CDS             49161..54218
                     /locus_tag="KQD82_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG4378991.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNHENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
BASE COUNT        17643 a         9403 c         6237 g        20983 t
ORIGIN      
        1 ttttatatat tattgatttt attaaaaaaa gagatataca aactaataag ttgtaatctc
       61 ttttaaactc taaattagaa ttactctaat atataactta caatataatc caatgtcctg
      121 tttatagtaa aaaccacatt taaattttag attggatgaa aattattaag attatttata
      181 aactcaaatt gttactctcc tttttcttat tatcttttga atgtattctt tagttaaaaa
      241 gttttacttt ctcatatatt ataaaggaag ataatttttt acaaataatc cacttaatag
      301 catccttact ttaaacctaa agttcctcta ttccaatttc ttttaagtat tcatacacac
      361 cattataaaa atttggctgc attgtaatat cactctcatc acctattggg acaaatataa
      421 taaagccttg ccttgccctt gtaagaagaa ctctatatgc attttttaag tatagtatat
      481 tatcttcttt attgatgttc tgccatttag ttccattgaa gttatagtat tcaaaatggt
      541 tattcttaaa tcttaagttc ccatcccaac agacaattgt ccagtctagc tctaaacctt
      601 ggatatcaaa ctctgttgca gtttcttcta aaaaatatga agctcgtaca tcatccttac
      661 cattaagaaa ccagtttgta gcatcaattt tattttgtac ccacactcca tactttcgaa
      721 gccgtttagc tccagaactt gcagtcattc cataacgctg tgaacctttt gatttatctc
      781 tcacccactt tttagctaat tccaaatccc tagtaacaca tatagggtaa tcttttttaa
      841 attgattata taattccttt gcagcaactt tatctacatc caaaacagct ttaacaaagg
      901 ttgatacatt ttcacttcta aaagaacgta acgatacaga taagtgcaaa tcatcaatta
      961 ttctataatt aacaccttct gttaactcat caaaagaatt acctttagaa tattcttcat
     1021 caataatttt atttgatatg taaacatccc aatcagaata attatttctt agtgattcaa
     1081 accaacctga tattccatct gactcacctt tatttatctc ttgacctcca ccaatcaaac
     1141 aaacaatagt cgcccatcct atatgacgat ttaaaatact aataagaaat tcaggctctg
     1201 acatattaaa gtctaatacg cctttttttc ttttcataaa gttagtcaaa ttaggttcat
     1261 cccacgcacg ttgtgcttca tcaaaaattg tgactctttc aataggtggt gtatcaacag
     1321 atatagcttc atctctaaaa tgatgaatta tctgaataaa ttctttcgct tttcgaatcg
     1381 cttcagattt tcgtattcca tttctggtta catcatcacg agctagtgct tcttgtaaaa
     1441 catcaactaa tggaccattt ccagaaagga agactgcatg ttcattttca tctacttttt
     1501 gtcgctcaat ggcaatatta aggccagcaa gtgttttacc tgctcctgga actcctgtga
     1561 taaaacatat tgattttctg ttatgagtct tactatagtc tataatttta tttatagctt
     1621 tagttgtttg atttaaattt ttagcacttg catcatttct tgaaatatct tcaacattat
     1681 ggcccatata taaagcttgt gcagcttcaa taattgttgg agttggcatg tataacgagt
     1741 ttatccaagt gtctgaatta aatgaacttc tattatatgt agtacaaatc ttctttatgt
     1801 attttcctat attaaactca ttacaacaat aagtttctaa aatattttcc ttcatcattc
     1861 ttattctttg gtctttttga ggggcttttg tacaaataag tattggtact aaaagtttat
     1921 catgactttc tttgtgaaaa caattaaggt caagggcata gtctgtaact tgttcaattg
     1981 catgcctagg ataagtgtct tcatcaactt taaattcaag gagaaaaaca atgcccttgt
     2041 aaattaacac attgtcaatt ctactgccta ttcgtggtat tgtatactca aataataagt
     2101 gtccatcttt gaaagttgag agctctcttt ttaatattcc aatttccttt ttccatgtat
     2161 tcttttgtaa gtcatcagcc gcaaattgat cattacacgt tatctcacca aatatagtaa
     2221 aaatatcttt gactataaat tcatttacac tacttgaata atatgctctt ttcatacatc
     2281 ttcccccttt atatctaata ttcattttat agcctacatc tatactacaa tctaaaactt
     2341 gactcctata ctttataaat acactacata gatatgttta tgtatggtac tttgataata
     2401 atttttcttt gctaagtaca ttataaacta tttaattaat atgttttttc catatatgct
     2461 tattatatta taaaatggat agcgcatcat ttaaatcatt gattaaatct tcactatttt
     2521 caagacctaa acttattcta attgtgtatg gtgacacatt taatgccttg gcttgttcta
     2581 aagtcatttc atttaatata gctatatttg gagcaaatac taaactttca tggcctcccc
     2641 aactcactcc tattttaaat aaatctaatt tatttacaaa ttttttaaca tcatttacat
     2701 cctttgcgtc tatttcaaaa cttaataacg cagtataatt aagcaactgt ttctttgcta
     2761 gttctttttg attaaaatat ttagataatg gatgatatac cttagtaact gctgaatgat
     2821 tatctaaaaa ttcaactact tccttagcat ttttggtatg tactgataat ctagcgtgta
     2881 aagttctcaa acctcttata actaaaaatg cttcaaatgg tgctaatttt gcaccatata
     2941 aagctaattc actccctctt atttcttcta tatctttttt acttccaata atacatccac
     3001 ctaccacatc tgaatgtcca tttaaatatt tagataaaga atgaacttct aaatcaattc
     3061 ccatctcaat acatctttga tataatggtg tagcccaagt attatctatt actgttttta
     3121 tattgtgtgt ttttgctagt gctacaattg cctttatatc ttgcatttta aatactgatg
     3181 ttgatggaca ctctaggtat attaaagttg tattttgttt tatagcattt tcaaaatctt
     3241 ctattctatc accttcaact attgtatttt ctatatttcc tttctccttc aattcctttg
     3301 ttataaagtt cattgtagga ttgtataaat tatttgaagc tattatatgg tcaccatttt
     3361 ttacaaaatg aagtatagca cttgatatag cacccattcc tgaaccaaag gttatacttg
     3421 cttcagcatt agctaatgca gataactttg cttctaaaat atcattagta ggatttttag
     3481 ttcttgaata aagatatcca tcttttgaac cttcaaaata tgcttctata tcttcatatg
     3541 tacttcttgc gaatattgat gattgatata taggtgggtt tacagctcca taatcgccat
     3601 ccaaattttc tcctacatga ttcattaatg tctctatctt atatttattt ttatccatgt
     3661 tctaaatctc cttatgaaat atttttatat atgaaaaata catttaacat agcttatttt
     3721 ttataagcta tgttaggtaa taaagttaaa atattcatta tgatacttta ttagattgtt
     3781 ttttatcctt tgaattttta tatgtaccta tccctgtaac tgctaagatt actgctataa
     3841 ttggagttcc ccagttcact atagaccaaa gtgcgtattc tccaacatta actcctagtg
     3901 tctgagtgaa aaatattgct gctggtgtcc aaggaaataa aacttctaac aatgtacctg
     3961 tatcttctgt agttcttgat agtaccctac gatctatatt attctcatcg tatttatctc
     4021 caaatatacc tgctgttaca aaactacatg ctataccatt tgcagtcatt gctatcattg
     4081 ctattcctgt taataatgaa gaacatatta tagccgttct agacttaacc cacccaaata
     4141 tcacatttac cgttgctggc atagcatcaa tacttccaag tactccaaca acaaataata
     4201 tagatgttga tattggcaat aatttaccta attcccagaa gccacctttt tggaaaaaac
     4261 ctaatatata agcttgtcct tctataggtt ttgcatattc atgccaagtt accatatcta
     4321 gtgagaatcc actgttaagt cctataagaa catctgccaa agaaaaacct tgaaagatta
     4381 ctgcaaatgc aagtgataaa aatgctgcta ttgtcattac tggtattgca ggttttttca
     4441 ttgctgaacc tactaatact attaaaagag gtataaataa aattatattg aaattgaaca
     4501 ttcctgataa atctgccaat aatctagtta tttctggact atttaaattt gtatttgttg
     4561 ctgggaatat aaacccacaa gcaatatata taactaaagc tattatagtt gctggacctg
     4621 ttgtccataa catagatgca acatggtcat ataaattaac ttttgacgct attgcagcta
     4681 tatttgttgt atctgatagg ggtgacattt tatcaccaaa gtaagctcca cctactatag
     4741 cacctgctat aatagcattg tttgctccta ttgcattacc tacccccatc attacaactc
     4801 ctatagttgc tgctgatccc caggaagttc ctgtaaatga agaaaacaat gctgacaata
     4861 ttaatgctgt agcatataca aatcttggat ttatcaattt aactccataa tatactaaca
     4921 taggcattaa tccactcata ataaatcctg caataagtgg acctattact aataatatta
     4981 ttaatcctgg taaagctcct tttacaccat ctaatccact ttcaaatact tcttcccacg
     5041 ttttatttgt tgtaaccact ataaatagcg atattattga tgctgaaata aacattattg
     5101 actctattgg caaactatat tccggcttat tgttaatcat tggaagtaat agcccacaaa
     5161 ataaaactgc cataaacaaa actatagata tagcctgctt ttgaattgta acttttctct
     5221 tcatttatta tttcctccca cttttattta ctcattaatt caatttttaa taacgttttc
     5281 ccttatctaa caataaaatt tactatctta tatttatatt caacaatata tttatatata
     5341 taaatatatt gttgaatatt ttattataaa ctccagttta ttcttaaact attttcttaa
     5401 gtgaatttac tttctatatc ttttaatcac ctcctctctc atagtgtttt attaattgta
     5461 cataataagt tatcatagct ttgtttatct tttatcttat ctagtacaaa taatcctctg
     5521 atattgaaac cttaaaattt tttgataagt ctctaaagtt atctcctgtt attgtttgtt
     5581 ttttccctcc catagatatt acttttacta atatctcagc cgatttttct actgtatcca
     5641 taagaccaaa tgttaagtcc aaagtttctc ctgagcagaa cgttccatga tgagcccaga
     5701 ttactatgtt atgtttttcc attagatttt gtgtagcttt tgctatctct atcccacctg
     5761 gcaccatcca aggtacgatt ccaattcctt ctggaaatac agctggacat tctgtagcca
     5821 tctcccataa ttctcttgta aatactttac cctcaagagg cagtataaaa gttaatgcta
     5881 tgagattagt tgtatgtgaa tgcattatta ctctatgtaa tccatttgtt ttgtcttttt
     5941 ttatagaatg actcattaag tgagatggaa attcacttgt tggtttagcc ttatctaatc
     6001 cccaaactat tgcataattt tcaccttttt catctatttt aacaattcct acattttgaa
     6061 cagtatttat agaaatattt ctcatgaatt tgccacttcc tgtaactaag aaatattcat
     6121 tagctaaatt ttcgactgtt acaccaattg gaatatattc tatttcttct gttataaatt
     6181 cttttattcc agatatttct tcaggtgtta atctatacgt tatattcccc ccattacgct
     6241 catgccatcc ctttaaccaa gcatcataag ttattttcat aaaatctttg ataaagttca
     6301 tttccattat agacatatac ccttacctac tttctattta ataattctgt taattcgtac
     6361 tcttttacta cttctatcca atcttcttta acagcaacat tattaatttt acaataatag
     6421 ttccatatgt ctcccattgg atatgttttg aactcttcca ttatagccaa tctttttgta
     6481 aaatcgcctt cgtcttgtaa ttttcttaat tcttcatgag gcattaacat agctgataac
     6541 aatgctttta tcatatttct agttccaatt acccatgctg ctatacgatt tatacttgca
     6601 tcaaagaagt ctagtcctat tattacttta tccaaagcat catttcttac tatttccttg
     6661 gctatttctt ttagttcatc atctaatata actacatggt cactatccca acgcactgga
     6721 cgacttacat gaagtgcaac tttatctgaa aataatagca ttgatgataa tttatctgaa
     6781 acaagttctg taggatgata gtgccctgta tctaataatg aaattatgtt gtttttacta
     6841 gcaaaattta agtaaaactc atttgaccca actgtataag attctacacc taatccaaaa
     6901 acttttgatt cgacacaatc taaattatat tctctgttta tctctacctt taatatttct
     6961 tctagtgatt cctttaagcg tcttctagga cctaatctat cacttggtat atctttgtat
     7021 ccatctggta tccatatgtt tgttaaacaa gtctgaccaa gctcctttcc aaagtattcg
     7081 cctatttttc tagaagctat gcaatgcttt atccaaaaat cacgtatttt tttatctggg
     7141 tgtgacaatg ttaacccatc acttgatttt ggatgagaga atattgttgg gttaaaatct
     7201 aatcccaatc ctctttcttt agcccatttt acccaatttt caaaatactc tggttttagc
     7261 tcatctctat ccacaaagct tccaccagtt tctgcatata tagcatgtaa gttaacttta
     7321 tgtttccctg gtattaaact taaagcctta tccaaatctc gtcttaactc ctgaggattt
     7381 ctagcctttc caggataatt tcctgtagat gcaattcctc ctgatagtct ttgttgattt
     7441 ccttcaaacc ctgttacatc atcaccttgc caacaatgaa tagatatttt aactttagat
     7501 aaaactctta atacttcctc tacatttatt ccccattttt catattcttt tttagctact
     7561 tcataccttt gctttaattc catcacttat ctcctttttc agattaatta tattgaatta
     7621 tatcaaatga atttcttatt atatctctcg cctgttctaa atcatttagc tcattcttag
     7681 caattaattg acatactata ttacctaatg ctgtagcttc aacaggtcct gctaatacct
     7741 ttttaccact ttcttttgct ataagctcat ttaacagttc attttgacat cctccaccaa
     7801 atacacttat agtttctaat ttttcttcta tgattttttc tatttcattt atagatttac
     7861 tataactttt tgctaaactg ttaaaaatac aatatgctac ttctccaact gtttgtggtt
     7921 ctgtttgatt agtttctata cagtatttta ttatttcttg taccatattt tcaggattta
     7981 aaaatctatc atcatttaca tctactattg atttaaatcc ttctgatagt cttgcatgct
     8041 ctactagttc tgtaaaagag tgcttactgt cataacactt tctagcttct tgtatcatcc
     8101 ataaacccat tatatttttt aaaaatctat atctatattc atatccacct tcattagtaa
     8161 aattataatc taaagcctct aatttgcact ttggtctttc taactctact cctaataaag
     8221 accatgttcc tgaactcaat aaaatgcttc tattttttac aggagtagac atgaaagctg
     8281 aacctgtatc atgagtaact ggtaaaatga cttctaaatc acatcctaac tcttctacta
     8341 attctttctt taaactacct agtattgttt tgggagtttt tattttagca aataacatct
     8401 tgtcaaatcc aagtttattt attatttctt catcccaatt atttgttgtt gtatttacta
     8461 attgtgtagt tgttgcattt gtatattctg taactttttt cccagttaat aaaaagttaa
     8521 aataatcagg aatcattaga aagctttttg ccttttctaa taaatctggc tgttgatttt
     8581 ttattgctaa taattgatat atagtattaa acttttgaaa ttgtattccc gtttttaaat
     8641 atagttcctt tttatcaatt attttaaagg cttcttccat aattccatca gttctattat
     8701 ctctgtatga tacacagtta cctaatatat tgtctttttc atcaagtaac acaaaatcta
     8761 cagcccatgt gtctatagcc acacttactg gaattttatt tatttttata cattttttta
     8821 tcccattttt tatttcagaa aatagctttg atatatccca acaaaaatga ccatcttttt
     8881 taatcaaatt attcttaaat ctatatactt cttctatttc caactttcca ttatttatat
     8941 aagagataat atgtcttcca ctagatgccc ctatatcaat tgctaaatag tatctcaaga
     9001 cttttcctcc ttagatttat ttattaattg aattatacaa gatgttagta tattcattta
     9061 atatctaaat ttgtcatatt tattaaccta atttgcaata taactttatt ctcaataata
     9121 attttcaaaa tataatatct tcagagctat taaaatggtt tatttaataa taaaaaagaa
     9181 tctattttat agattctaat tttactattg actatttttt atattttttt tgtattcttt
     9241 tggtgtaata ccatacttat ttttaaatat tttataaaaa taagttaaat tttcataacc
     9301 tacattttct ataatttcag ctattgataa attagtagat actaataatt cacatgcttt
     9361 acttaacctt ttttcttgca ctaaatcttt aaaagtatat ccaagatgtt tttttactag
     9421 tttacttatt ttataatcac cttgattcaa attacttgca atttcagata aactaccttt
     9481 cttgtagtcc ttatcaatat attttagtat ttctataatc atcatctttt cataattatc
     9541 tactgcatga ttttctacat cttcagaatt tttaactaat tctactaata ataatcctac
     9601 taataattta atctttacct tattaaccac acttggttca tacaattcta ttataatttt
     9661 ttctattata tcttgaatct cttctttctc agatacttta aaatatatat attctccaag
     9721 attataatta gtatataatg tagtcattaa aaagttcatt attttattat cattttccaa
     9781 caaagatata atataatcaa aaaactctgg ttttattata aaattaatta ttatatcttc
     9841 ttcgtcacta gcttctattt catgaataac atgctgattt aaaaatatta attctccttg
     9901 ttttaaatta attttatatt catctatagt ttgtactaac tttcctctat aaacataatt
     9961 aaattctata taattatgct tatgttttgg aaattgtgca aatcttgtat gttttcttat
    10021 atgtatcaac tcatctatat tcaataattt gttgctatct attataaatt gagaattatc
    10081 agtataaata cttttatcta ttatataatt tccttctaat atattttttt cttcttctgt
    10141 tatttccctc aacttatcat gaatatattg tctcatgagc tacctcctta gttagttcaa
    10201 ctactagttg tttcaattta aaattatttt tagcattaaa tatataataa ttatcacatc
    10261 tttttccttt gtgtatagtt cctgataaaa aattatatat taactcatta gatgaaactc
    10321 tataaagtta tgtttatgtt tgggtatatc tataaatcta cgatgtttag atattataat
    10381 gtcttcatct aaagaaagta tatttttatc tgttagtact atagttccat ttatttcttc
    10441 ccaacataaa atagtatgca atatagacac tattttttta ttatctagtt tttttacatt
    10501 cttagtaact atgtattcta agatgtacct ttctgcttgt gttttttgtt taagtatatc
    10561 ctctagcgtt tcatctttca tttatatccc cccagattta gctatacttt attacagttt
    10621 taaattatat tacaaatatt ctttatctaa ttgtatcata ttagtttttg acagtaataa
    10681 tgttaaatta tgacatttaa aaataccttt ttttgtttta aaatacattt ataggtaaat
    10741 acttaaaatt taggaggtat ttttttatga atacaatacg caaatgtttc aagatgaagc
    10801 tttttaaagg atttgaaaat gaatacaaga aacgtcatga tgaattatgg tctgagatga
    10861 tagatatgat tcatgaatat ggtggtagta actattctat ttttctagat gaagaaacta
    10921 attatttata tggatatata gaacttaaag atgccaaact atgggataaa agttcagaga
    10981 cagcaatctg caaaaaatgg tggaattata tgtctgatat aatggaaact aatgaggata
    11041 atagccctgt atctatagaa cttaaatcta tgttttattt aaaataagga caaaaattat
    11101 tataaatcac aaaatgggga tatatcaaaa taaaaatttg aattatctcc tttttgtata
    11161 aaaaagataa gtgattccta aaaacctcta gtctatgtga taattgttct gtgaaaaact
    11221 aaaaacatat ttcgattata gtagctaaag atgttataaa caagtggtat ttctactaga
    11281 cattgaaaca attattccca aagatgattc agtaagacta cttaacttta gaataattga
    11341 tatatcttgt gttgtttata ttaaattttt aatttaagta atagtatatg taatctttag
    11401 tcatcttctt accagttttt tatccacttt tctaatgaag ttattttttc atatgttaat
    11461 tcctcttgaa ataatgatac taaaagactt tttataaaat tataacttaa aagtttatat
    11521 atttttttga tatgaattta tagtacttat cttctttaat tgagattgtg tatatttaac
    11581 taaacattac aacaataatt ctatgaaatc aaatcaatta tttacatatt tttttagaac
    11641 aataataagt atatattcac aaaattttgt gttaatattt ttagtttgtg atataataaa
    11701 agcaaggaaa catattttac ttaactttga gtgatgtttc cttcaacaaa ttattttgaa
    11761 gatttacttt tctggaactt tatattaagt tcccagccac tcttagttgg tcgctcagag
    11821 tggctcttta atttatcgta aatgtaactg gctataacac cagttataat actcatcatt
    11881 agattttcca tagttttcac ctcctctcat aagaaagtga agaaacttcc atggaaacac
    11941 cactcatagc tttttcaaat atttattcct tgccatatta attatatcat aaatattgga
    12001 aacataaaat tttaatactt atcgtcttta ttattatttt ttataattaa taattttctt
    12061 tttaataata cataatataa atttatattt cttcaataaa aaagacaatc ttaattaatt
    12121 gtctttttta atatatattt ggctaataca ttattttaca caaacattac aagggtctat
    12181 tttaccttgt gacttagctt gttttaatgt tactttattt attgttttac ttctttttag
    12241 tgtagaacaa ttttttgtta gatgatatga ttttccatta ggagttacat atactgtact
    12301 tttatcagtt aatccaggat tattagagct aggctcatta cttggtttgt tcgtattatt
    12361 tgaattagta tcttttacaa tacctgtatt tgaatcccca acattattag ttatatttaa
    12421 acattatttt ataacaacat catcaatatt tccaatagca gttattttac ttgcattttt
    12481 taatatactc ttattacttc catttccaac taacactata ggtgtttttt gtgcaattgt
    12541 agaaccaact aaagaataaa ctaaatcagt cccacttgct atgtaaaatt cttttgagcc
    12601 attataaaat ttatttatta tttgtttatt tgtatcgtat ctatctactc cacctattct
    12661 tgttgcttta gtttcattta ttaacttatc actcatactg acttttccac caattgcata
    12721 agtttctaat ccatctgtag caaaacttgt agtatttcca tcagttaata ctattggaga
    12781 tttatctcta actgctatag aagcaacact catagcatct ggttcgcctt taaaagcatt
    12841 agttaatatt acttttttaa ccgtacttac tcttcctatt tcttttgcaa ctttatgact
    12901 tgtatctatt ctatcagttc cacttattct atttatgctt atttttttat cacttaatat
    12961 tttctgaact gatgctccta tcgaattttt tcctccaatt agatatactt cttttacctt
    13021 ttctaatctt tttaatgttt ctgaagggat tgtatctttc ttagttaaca atattggggc
    13081 atttttagct cctgacaatc cacttgctga caatccatca gataatccat tttcagaatt
    13141 aataagtatt gcaatatcat aattttgttt atctgctatt aaacctgctg tttcatactt
    13201 atttgtacct tttattgaat taatagaact aagagcgttt gatgttggac aactagcaat
    13261 caataatgaa acagacatcc ctattgccaa tagtgttttg cttaatttca taatgaaatc
    13321 ctcctataaa ttttataata ttacttaaat attataattt tacataaaag aatattttcc
    13381 tttatgaaat tagtttatat ttcaaaattt tgtcactttt taaatctata tactaaaaaa
    13441 tagactattt gtaacagtct atttcttagt atatcaaata tttatttagg tttacgtttc
    13501 tttgtactag tttttgtata tgatattcca gttccaggta tactcaatgt agctgatttt
    13561 cttccttttg aattaactga cactctagca cctttaacac cagcacttat acctacactc
    13621 ttcttaccta aatttaattt gagtcctcct cctagattta tgcttttcct aaagttaaat
    13681 cccatagata taaccttccc ttctatattt taaatattta tacttaaaat tatatttgtt
    13741 atacacttgt catcattggt atatctagca tctatatctt tttacgtttt ttcttcttat
    13801 atactactcc agcaactaca gctgctattc cagctccaac cccataagta gcatttctac
    13861 ttttctcttc ctctcttttt tctaaacctt catcatatac accttttaac ttagtttcaa
    13921 tagacttcat ttcatctggt atactaaaat cttcttcatt gtaaccttga ttatatacac
    13981 tttcttttgc tttaggtaac ttaaccttac cattatcata tccttcttta aaccaattag
    14041 catatttaac attacctata gttgatgtgt caaatctttc tcctttgata gcactctcaa
    14101 atccttgagt agtgtaatca ttttttaatt gttcttgtat ttcgtcataa gcattattat
    14161 aagcatctat aaattcagaa tctatgtttt cttcaaatgt cattttttct ttatctttat
    14221 tgctatcctc tatcccttta gctttatatt cttctatctt tttagtctta tattcattgt
    14281 atccggttga atatccggta tcataagcat tttttaaaac ttctttttca tatgtattat
    14341 tagatttagc acctgtaagc cctaaattat atccagtctc tttagccacc ttttcttcct
    14401 cttctaattt agccttacct ttttcatatc cttctgagta ctttgattca tatgtatcac
    14461 tataatctcc actataattt gatgaactat aatttccttt atatccatct tcataccctt
    14521 gattatatcc tttttctcca acagacctct tttgcttttc ttcattatta accgaagttg
    14581 ttgttccaga actgcttcct cctccagtat aaggacaacc accattatta tgtaaatgag
    14641 ctggataacc accacaatga tagtgataac ttcctaatcc agatttattt ttattatcac
    14701 gatgacctcc tgatgaatca gttcttcctg aatgagcata cgaaaagctc cctacactaa
    14761 tcatagttat actcattgca acagctatta actttttctt aaacataatc tatttccccc
    14821 taaaatattc tatgatttta ttatatcagt caatatatta tgtttaaacc gttaaaattc
    14881 gacaatgtaa atatttgatg aattgtattt gtaaataaat cttttagcat atcttgaata
    14941 tctgtagttg ttttgacatc atattcttca ataagttttt taatatttcg tttttgttaa
    15001 acataaaaag cgtgcacttt cctttgtata gtgatatttg atattttaat tatcactact
    15061 ttagaagaat acacacttta ttttaaaaac cctgatacta tgttattaga aaatggtact
    15121 aatataaaag atatccagca tatactcgga catgctaaaa cttcaacaac tatggataca
    15181 tatttacatg taactaataa catgagaaac cagacggtaa atatttttga aatcattgta
    15241 gaataaattt gccacccaaa aataattatg gtagcaaatc tacttttaag acacactata
    15301 aaagtctgaa accctgttat tctctaataa gtttaactac agtctcacaa tgtggcgtat
    15361 ttgggaacat atccatacac ttaaccatct taatctcata accatacccc ttaaaatcaa
    15421 ccaaatcaac aaccaaagac ttaggattac aagaaatata aacaatctcc ttagcaccaa
    15481 atccacaaat atccctaata gcatccttat gaatgcctgg tctaggaggg tcaacaataa
    15541 tcaaatcagg cttgtccttt aaatccttaa caaccttagc cacatcccca gcaataaact
    15601 cacaattagt aagtccattc aacttagcgt tctcattagc agcaacaaca gcttcctcaa
    15661 ctatctcaat accatacact ttcttagcag caccagccat aacctgtcca atagacccag
    15721 taccactata caagtcaaat acaactttat cattgtaatc tccaataaaa tccctagcaa
    15781 tactataaag aacctcagct cccttagtat tggtttggaa aaacgaaaat ggtgaaatct
    15841 taaacttaag acctaaaatt tcttcttgta tataatctct tccatacaat actcttaact
    15901 catcacattg gactgcatct gctaaaccat cattaatagt atgaagtact cccaccaact
    15961 cagattttaa atcaatccct aaaagcatat ctttaaactc atacatatca aaatcttctt
    16021 gagaagaagt tactatatta accataatct cattagtgtg aatccctttt ctaactacaa
    16081 gatgtctcaa atatcctttg tgattcattg ttctataata aggaagcttt ttttcattga
    16141 aaaactctac actagaaact aatatctttg agaagtcttc atcaaccaac atacactcct
    16201 caacagtttg tatatctata tgctttcctt ttttatgaag acctaatgtc aaaggtccat
    16261 tttttacttc atctccaaaa gtatattcca tcttatttct ataatatttg ttttctggac
    16321 ttccttctat ccctaaaaac tgaaatccaa ataaatcttg ctttaaaaat aaatccataa
    16381 cttgtttttc ttttatttct aattgttttt catatggcac agacaatagt gtacatcctc
    16441 cacactctct aaagtgcttg caagtttctt cagtttcaag tggtgagttc tctaataatt
    16501 ctatcatctt aacttcagct ttcttacttc taactttttt aactccagct ctcacctttt
    16561 gaccacttat tccacccttc atatggataa ctctatctcc aacttggctt aaagatgtac
    16621 caccaaattc cattttatct acttcaaatt ctataacatc ttttttcttc acagacattc
    16681 ctccaattta ctatagtatc tttattatct ttcttcaatc aattttactt cttcatctgt
    16741 taactctcta tactcaccta actctaaact ttcatccaat tttaaattgc ccatagacaa
    16801 tctttttaag tatactactt ttttgcctac gctttcaaac attcttttta cttggtgaaa
    16861 cttaccttca tgtattgtaa gctctatttc tgattcatca tcacttttta atatatttaa
    16921 ctgtgatggc atagttttat atccgtcatc aagcactaca ccttctaaaa aagcttctac
    16981 atcttcttca gtaactactc catcaatttt tgcataataa gtcttaggca catgcttctt
    17041 aggagataaa actctatgag aaagctttcc atcattagtc aaaactaata atccctctgt
    17101 gtctttatct aaccttccaa caggaaatgg ttcaaaaact aagtatgata aatcaattaa
    17161 atctaatact gttgggtcat atttatctgt agttgctgat atatatccag caggtttatt
    17221 catcatcaaa tatatatatt ctctatatat aacttcttct ccattaaata atatctcatc
    17281 attttcagtg tctacttgag ttcctggatt tgaaacttca cttccattta ctactacaga
    17341 cccttgttta caatacttct ttatttcact tctgcttcca taacctaaat ttgaaagaat
    17401 tttatctata cgttgctttt tagccattaa aaatgctctc cattctcata atatttttct
    17461 ttttcaaata ctgtaaatgc taaatccata ctttctacat ctaaattatt tcttatacta
    17521 tcagttatta ttcttgctac tatatcttgt acttcttgtc ctctatcaaa ccaagctact
    17581 tcaacaaaag gatatacatc tgctatttta ccatctctta ttgcaactga ctttatacat
    17641 tctatttcaa agtagtctct tggacatttt acagcttcaa ctaaatcatt tatcattttt
    17701 tcacttattt tacatatatc attttcatta attcctctta tttttatctg tggcatagtt
    17761 tatctccttt attttaaatt tttagtgaat tatcatccac ttaattccct tcgataacgt
    17821 ttgaagtggc aacttcttgg tcaagacaac tattgttatc agtatctcca aactaataag
    17881 gtagttccta cccttatagt tagcattata tagctaattt tcctaaattg tttatattct
    17941 ttattatttt atttaatcct attgtcttct atttaacatt atacttgtaa tttatagtta
    18001 ttacaattta ctatgtactt tttttgattt aacgttataa tataagtgat gggtaaattt
    18061 taaaagttaa taggaggagt ttaaaatggc attaattact acaaaagaaa tgtttaaaaa
    18121 agcttacgaa ggtggatttg ctataggtgc atttaacata agtgacttag aacaattaca
    18181 aggtgtttta aaagctgcta aagctaaaaa ttcttatgtt atgatacaag cttctatgtc
    18241 agctgttaaa tatgctggac ctcatacatt agttgaaatg gttaaggctg cttctgatga
    18301 aataggtgta gatgtggctc ttcacttaga ccatggtcca aatatggatg ctattaaaac
    18361 ttgtattgat gctggtttct cttcagttat gatagatggt tctcattttg actttgaaga
    18421 aaatgttaga ataactaaag aagctgttga atatgcacac tctaaaggtg ttgttgttga
    18481 agcagaactt ggtgttctag ctggaacaga agatgatgtt acttctgatg ttcacaagta
    18541 tactcaacca gctgaggcag ttgagttcgt tgaaagaaca ggtgttgatt cattagcaat
    18601 agctattgga acttctcatg gtgctttcaa attcaaagga gaagctaaat taagattcga
    18661 tatactagaa gaaatccaaa gcaaattacc aggtttccca atagttcttc atggtgcatc
    18721 tgctgttgac caaaatgctg ttgctacatg taatgaattt ggtggaaata tagctggtgc
    18781 taaaggtgta cctgtagata tgttaagaaa agcatcttct atggctgttt gtaaaataaa
    18841 catggatact gacttaagac tagctatgac tgctgctatc agaaaattct tagctgaaaa
    18901 tccaaaagaa tttgacccaa gaaaatacat tggtgctggt agagatgcta tacaagctgt
    18961 agttgaaagc aagatagatg atgttttagg ttctgctaac tctataaact aatataactt
    19021 ttaaatattt ataaaagaaa agagatgact taaagtaatt aaatttattt ttaagccatc
    19081 tcttttaagt tgtataaatt atgtatctta taatatttta attatattgt ctttttaaac
    19141 tgacagctaa acgtgaatat gtccactctt tatctttcta tggatggtag aaggatatat
    19201 acctataact tcagcagcct ttgttattga accatactta tttatagcct tacttatcat
    19261 tatttggtcc actattttat atgcatcatt aagattcata attccattta ccactattgg
    19321 tgataaatca tctgtttcat tatcaatcat atctaaattt atcagcatgt taaattcatc
    19381 ttctccaatt acatttttag ctgataatac tacaaatctt tcaatgatgt tttttaattc
    19441 tcttatgttt cctggccaag aatattcttc taataactca ataactttag gcgaaacttc
    19501 tacttctcta ttgtactttt cattgtaaag ttttaagaaa taatttacta atggtactat
    19561 atcctctttt ctttctctaa gaggaggtat ttttactgga ataacactta atctatagta
    19621 caaatcttgt ctaaatttaa ggttatttct aaggtcttca tgagaaatat tagtagcact
    19681 tatatatcta acatctattt ttataggctt actaccacct attctagtaa tagtattttc
    19741 ttgtataacc ctaagtaatt ttttttgcat ctgcattggt aactcaccta tctcatccaa
    19801 gaaaatagtt ccaccattcg cctcttcaaa aagtcccttt ttaccttttt tcttagctcc
    19861 agtaaaagaa ccttcttcat atccaaaaaa ctctgactct aataattcat ttggtatagc
    19921 tccacagttt atagctataa aaggtttgtc tctccttgaa ctatacttat atattgttct
    19981 tgctattatt tcttttccta cacctgattc tccatttata aatatagaag aatctgaggc
    20041 tgcagctctc ttaacaactt tttttatatt gtccataatc ttactttgac ttatatactc
    20101 tgtgtcttct gatatatcac tatattttat ctgagattca agcttaataa cccttgaaat
    20161 atctctccct gttagtactc ctcctgtaag ctttccctta gaatcccatc ttggaactgc
    20221 cgtatatgca ataagcttcc catctgggta aacgatattt ctataaatct ttttcttcac
    20281 tttaagcatg tttaatatta ttggttcttc aactaatcct tgtctcacaa ggtctgttat
    20341 atttctacca agtacctcag ttcttttaaa aggtaatatc tcatcactga cagtgttcat
    20401 cttttcaatt accccatctt tgttaaacac aacaacaact tcatctatat gctctattat
    20461 atcttcaatt actttatcag atattagaga cttctcaaat aaataaacta tattattttc
    20521 attattatta tctttcatat tgtatatatg tgcagtcatc cttaactctc tgaaagctat
    20581 tttcttttta ctatctgtca gtatttctgt tttgtcaaaa tttgaatcta attcatttat
    20641 atttctaggc ttttttatat ctagaaaact aaaaatacta tctattagat gattacagta
    20701 tattatttcg cctgattctt tacataaaat ggctggattt tctacatgtt ctataagccc
    20761 tattaaatta ttatccatta atattcgcac ctttcattaa gtaatttatt gaactacata
    20821 caaaatttat tgtttgctta caataaatac catcagcaca tataatcaac tccttataat
    20881 attcgtcttt tcaagtagtt tatttcatta aaacgtaact aagatataat tttaatgatt
    20941 atagtttgtt aaaaatttca ttttgctttt aattgtattt tatcacatat ttagtaatta
    21001 tttgtctatt taatacaatt taattaaatt taagatttat ttaaaaaaac aaagataatc
    21061 cttacagaat ataactcaca atttgtaagc aatatacata taaggattat ccagattatc
    21121 ttgtattata aaattttatt caaacagact ttttaataac tgacaaatac acactagcaa
    21181 taacttatca aagggttaat aattatgtta ctaactaata agacttattc aatcagtaat
    21241 atttatatag tttgtatcta ttaagctttt atagcttcta ataaatcatc aacactatct
    21301 aatttttcta taagcattgg aactatttca tgtaagtctc caactactcc atagtcagcg
    21361 atttctaaaa ttggagctgc tgggtttttg tttatagcaa ttatgaaatc tgatgattgc
    21421 atacctgcta aatgttgtat tgctcctgat ataccacaag ctatatatag gtttggttta
    21481 acagtagttc ctgtttgacc tacttggtga gaatggtcta tccatccagc atcaacagcc
    21541 gcacgagaag aaccaactac tccacctaat ttgtcagcta atttcttaag catttcaaat
    21601 ccttctggtc cacctaatcc taaaccacct gatactataa catttgcatc tgtaagagat
    21661 actaaatctt tcttagtttt aactgtttct aaaacagtag ttcttatatc atcttgagtt
    21721 attttgtagt ctaatgctat aacttcacca gttcttgttt cgtctttttc agctttatcc
    21781 ataacaccag gtctaactgt agacatttga ggtctatggt ttggacaaat gattgtagcc
    21841 attatgtttc caccaaatgc tggacgagtt tgttttattt tcttatcttc tgggtctatt
    21901 tctaacttag tacagtcagc tgttaatcca gttccaactc ttgaagctat tctaggtgct
    21961 aagtctctac ctatatgagt tgctccgaaa agcattattt ctggttttat ttcatctatt
    22021 gcatctttta ttacttttgt atatgcatca gttgtatatt tttctaaaag agcatcatct
    22081 gcaacataaa ctttgtcagc tccaaaagtg attaattctt tagctaatcc atcaacatct
    22141 tttcctaata atattgcaca aagttctgca tctacttctt tagctaattt tcttccttct
    22201 cctaataatt ctataactac tggagctatt tttccttctc tttgttctgc aaatatccat
    22261 acgtttttat aagaacttaa atcttttata tcattcatag tttgtactcc tccaacttat
    22321 ataatgtgct tttgttttag accgattatt attttactag ctatttcatc agctttacct
    22381 tctaagattt ctccttttcc tcttggttca ggagtaaatg atctaaatac tttagttggt
    22441 gatgctttta aaccaacttc ttctttattt acatctaaat catcaattgt ccaaactttt
    22501 acatcatgtt tatatgcttt tactatttta tcaacagaca tgtatctagg tgtatttaac
    22561 tcttttactg ctgttaaaag tacaggtgta cttactttta ttaattcgta tccatcttct
    22621 aattgtcttt gaactattat atcttttcct tctattttga aatcttgtac atatgtaact
    22681 tgaggaatgt ctaatttctc tgcaatttgt ggtccaactt gtgcagtatc tccatctatt
    22741 gcttgtcttc ctgcaaatat tatgtcatag tcacctactt tagatattcc cgctgcaata
    22801 gtatttgatg tagcccaagt atcagctcct ccaaatgctc tatcacttaa taatattgca
    22861 tcatcagcac ccatagctaa acactctctt agcataaagt ctgcttgagg aggtcccatt
    22921 gttataactg ttatagttac atcatcatgc tcatctttaa tttttaaagc ttcctctaat
    22981 gcatttgcat catctgggtt taatatactt ggaactccat ctcttataag agtacctgtt
    23041 tctttattta ttcttacttc gttagtatct ggaacttgtt ttacgcaaac taatattttc
    23101 aaagtctata cctccaattt cattagatta tttattattt taatacgttt gatgaaatta
    23161 ccattttttg aacttctgat gttccttcgt atattgatac gattctagca tctctgtaca
    23221 ttctttcgat ttcataatct tttataaatc catatccacc gtgaagttgt aatgctttat
    23281 aagcaacttc atttgcaatt tctgatgcat agtatttagc catagcagat tcttgagcaa
    23341 ctctcattcc tgcatctctc ttttgtgctg catcataaac taatcctcta gctgcacaaa
    23401 cttttgtttc catgtcagct atagtaaatt gagtattttg gaatttagct ataggcttac
    23461 caaattgaac tctttgtttt gtatatttaa cagcttcatc taaagcacct tgagctattc
    23521 ctagagctaa ggcagcaaca cctattctac caacttctag agtacccatt gctatcttaa
    23581 atccttgacc ttcttttcca agtaagtttt cttttggaac tttaacgttt tcaaatatta
    23641 agtcagaagt ttcagttcct cttataccca tcttatcttc atgagctcct gttgagaaac
    23701 catcccattt actttctact ataaacgctg atgttcctct aagtccttta cttctatctg
    23761 taactgctat tactatagct acatcacaga atggtccatt agttatgaaa gtttttctac
    23821 catttaatat atagtggtct ccaactaatt ctgctgttgt ttgttgtcca cctgcatcag
    23881 aaccagctcc tggctcagtt aaaccaaatg ctcctattaa ttcacctgtt gctatacctt
    23941 ttaagtattt ttgtttttgc tcttcagtac cattgtaaag tattggtaat gaagcaagag
    24001 aagaaccagc tgtaacaaaa gtagctgttg aagcacatct tctagctatt tcttccatta
    24061 ctatagcgta agaaacatat cctgctcccc caccaccata ttcttctggg atattactgc
    24121 ttataaattt tgcatcagct aattttttga ttaattcctt aggcattaca cctgttttat
    24181 ccatttctgc tggtaaagta tctaattctt tactaacaaa atctcttact gcttttctta
    24241 aaagttcttg ttctttgtta tataacatag cttcctccaa ttttcatatt ttatagtaaa
    24301 tttattttaa tgaacttacg ctaaactcat catctcagca aaagtttgaa tacgagttct
    24361 ggcttgttca ttattctgag tttgttggtc gatttcaaca tataaagtag gtattccact
    24421 atcttctata tcttttctaa ctaaaggata atcgtattct tctgggtcac agaatttcat
    24481 catacagaag ataacaccat ctatatcttt ctttttaact tcatctacta taagtgaccc
    24541 acgttttttc ttagggtcat aagctaatga acatccttct atgtttgacc attgtcttgc
    24601 taatctctct aacgcatcat cacctgctgg tacatctgtt ctgaattgtc ttgtttcttg
    24661 tgctaagtcg tcagctacaa ctgatatatt gttgtcttct aaaatgtcta atatatcttt
    24721 agaatcagct aatatacctg ttaataaaac tttctttcca gaacagactt cttctggcat
    24781 agcatttaat tttgctatta aatctttcac tagctcagta tgttcttctt ttctcataaa
    24841 gaacccactc ttaattacta agcttcttat tgatggtttt atagtattag aatgtttata
    24901 agctacttca acaaagtctc tcatagtttt tctatgttca ttgtaaactt ctatactttc
    24961 atgaattttt gcttcttcta tttcatatcc acaaatttct tcaagttctc tttttacgcc
    25021 tttgtactca ctgattaagt attttacacc agcttcaagt tttctatttt gtgggtgtac
    25081 taatgatata tattttatat gaggtactgc tgatttccag ttttgtccta aacaaattag
    25141 tgtatcacac atacctggta taataactcc agataattca tcataagcac cttttaatcc
    25201 atattctaaa catgattgca ttattgaaca tgcaaatgca gggaaatatt gtttagctaa
    25261 atctaattct gtttgtcctc cccatatacc aactggaagc attccagctg catgtataat
    25321 ttcttctggg caataaactg ggaaacaacc tatagctttt ttaccagttt cacttttata
    25381 tttttttaca gccgcatttg gattttcaac tacttctttc attttagata aaatagcttc
    25441 cattatatct cacctctatt aagttttttt ctttcttcca ttacttcaac taaaccttga
    25501 attcttgttt caaattgagc attagtgaaa gctctagggt ctgcttggtc accatcaaat
    25561 ccagcatatg gtaatccagt ttcttcagct gctcttcttt gcatttcata ttgtattaaa
    25621 ctcataagtt tacagcttct gttcatatga tagaatgctc catcacattt accctctact
    25681 aaagaatcaa ctctatattt tgtcatacgg tctaggttta cattgttaaa catagtacta
    25741 tatgctactg ccattccatc taaatcatta acttcatatt gtaatgccca agcatgtggg
    25801 taaacactac ctgtcatgtt aactccaaat ttagctaatg ttttcatctt gtatcctata
    25861 tatggccaac aaggtatacc ttccatcatt attctgtatt tttcttcccc tctgaaagaa
    25921 gatttaccag ttttcatgtt gtcctctaat tcttctataa gtaacttaaa tgcttctgta
    25981 gtttcttttt tacctctagc acaaactatt acagccatgt aagtaaataa gtcaaatcca
    26041 ttcattggag aaggagaaga atctgctggt aaactcatag aatacttcca tagtcttccg
    26101 ttttcagctg atattttcat tacttcttca aacttcttag ggtcaaattt ctttcctgat
    26161 ataatttcta gttgttttat agcttcttca aattgagctt taatataatc tattcttgat
    26221 tgagtaactt cgtcttcatt attgaaagtt gtatcaatca ttattaaagg tatatctaat
    26281 tctcttgaaa tattttcata ccattttata acttggttac atatattgtt acagcaaagt
    26341 aggaaatctg gagctggcat atccaaagct tcacatccac cattttctaa aagaccaaaa
    26401 tttgttcttg catatgcaca taggtcaata gaatatcctt tagattcagc tatttcacat
    26461 aattctaaag aacctttttt agctgctact ccagctgctt ggttttctgg atataatacg
    26521 tttaagtcaa atacttctgc taactcttga ggaaatactg atgttgacca acctacaggt
    26581 cttccttctt ctttagcttt aaatgcattt gcatattgtt cagctaataa atcattaatt
    26641 actactctag cttctttttt ttcagacatt ttcatccctc tttttatata tttttcactt
    26701 ctttttgtga ttcttttgct tcatcaaaag catataacgc tgctcctaaa gcaccagtta
    26761 attgaggtat atcaggtaca ataatttctg tgttgatttc tcttgccata gctcttacaa
    26821 taccactatt tctagcaact ccaccaacca taactacatt tctttgtact cctattcttt
    26881 ttactaggct agaaactctc tttgctactg aagtatgaat acctgccact atatcttcaa
    26941 tttttgcatt ttcagataaa tgtgatataa cctcagactc tgcaaatact gtacatgtac
    27001 tgcttattga tacttcattt tgagaattca tagatatact tccgagttca gatacatcaa
    27061 cctctattat ttttgccatt acatctaaaa atcttcctgt acctgcagca catttgtcat
    27121 tcataagaaa gtttaatagt cttccattat tatctaattt taataccttt gcatcttgac
    27181 cacctatgtc aataatggtt cttgtctctg gaattatgaa atttacccct ctagcatgac
    27241 agcttaattc acttatttgc ttatcagcat ctgaatagtt cattcttcca tatcctgtaa
    27301 ctacaacttt tttaatatct tctcttgcaa gacctgtctt gccatataat ttttctaaaa
    27361 ctcttgatgg tccagtagtc ccagtaccag aggatattgt ttcagaagct acaatatctt
    27421 ccccattctt taagattact ccctttgatg cagttgaacc tatatctaat cccattgtgt
    27481 acatctttag cattctcctt ttaaaaaatc tagaattact aattaatatc ttacaacttt
    27541 actatcttta aagttattta ttttctcttc atcgtagcct aaagatttta atacttctac
    27601 agtatgttga cctacttttg gtgctggtgt atattcttta attccttcat ttctaaacat
    27661 aactggagta ttaactaaga cacctgtatt tccgctatcg tatgttttct taaataagaa
    27721 gtcatttgcc caagcttgtt cgtcatctaa taaatcttca cagctttgaa ttttttcaaa
    27781 tggtaagtct gcttcttcta ataaagctga ccactcgtct aatgtttttt ctaacatagc
    27841 ttctccaact atcttaacta aatcttcaac atgattaacc attgaatcta tgttattata
    27901 tctatcgtct tctaatatat attctctatt tataacctta cagaatttgc ctaaccactt
    27961 gttgtattgt attaaagcta attgaatcca tcttccatct ttacatttat atgtagtcat
    28021 taatgggctg tttggatttt ctcttgataa aggcatttca tttccgtatt gtgctgttgt
    28081 tatcattgtt cccattccat atatagctgt atggaaaaga cttactgtta ctctctcacc
    28141 tttaccagtt tgagcttttt tatgtaatgc tgctaaactt cctgctgcta gtgctagacc
    28201 tgcatagtgg tcaccaaatc ctgctgctgt atttgctgga gatgttcctt tttccataac
    28261 agattggcta actcctcctc ttgcgaagta tgcagtatag tcaaatcctg gtttatcttt
    28321 taaaggtcct ttttcaccat atcctaatat ttgagagaat attaatcctg gatacttatc
    28381 ttttatttgg tcataagcta tacccatttt ttctaatgct tgaactctaa cattagtcac
    28441 aaatatgtct gcttctgata ataatttatg taatatttct actccttctt ttgattttac
    28501 attaatactt acaccctttt tatttccatt ttctaattca aacatagggt tttcatcatc
    28561 tgatgctgga gatttaaatg ttccacccat aactcttatt ccatcacctt ctataggttc
    28621 aatcttaata acctctgcac cccagtcacc taacattttt gcacaacatg gtgctgcgat
    28681 gaaacttgaa agttctacta ctttaactcc ttctaaaagc ataacgtttt cctccttaat
    28741 agtatcaatt ataaaatttc gattaaatca ctcataaaat acttttttct gatattaact
    28801 gtctgatgtc aaagttccta atcttatcta aaataactta aaaattatta aaattatttt
    28861 ttattgtttt attgtcttac acctatattg ttaagcattt atcgtgccaa ctttatattt
    28921 aatcaaaata tcgcttgatt tgaatgtttt ttaacaatat gcaattgaat aatctttttc
    28981 atatcgtggc atcttgccac tatccattgc attttgctat tctttctctg cttataaaag
    29041 ttttttttat atattttttt atgaaaaagc ttgacatttc aaaaaaagtt ttcattgcta
    29101 aactcaaaaa cacatgttat cattgtaatt aatcagcaag ttagttaact aatttcaaac
    29161 ttagataatt ttttaataat atttaatcta aaaaataaca atttctttga taaattttag
    29221 agtaattatt acattaaata aaaaattact aaatatcaaa ataacacagg gaaaacaatt
    29281 tcaaaactaa atttttaacc ttaaaaattt tgtataaaaa atataacctt atatgctaca
    29341 tattatttct aaatgaaaaa ttaccataat aatttaataa ttacaggagg gtattgatat
    29401 gaaaatacta gtatttggag cacgcgatta tgaagaacca gtaataaaaa aatggtctga
    29461 agaacataag gatgttcaag tggatattta tcctgaaaac atgactgaag aaaatgtagt
    29521 taaagctaaa gggtatgatg gtatatctat acaacaaact aactatatag ataatcctta
    29581 tatttatgaa actttaaaag atgctggggt taaagttata gcttcaagaa ctgcaggggt
    29641 tgacatgata cattttgatt tagttaatga aaatggactt atcgttacaa acgttccttc
    29701 ttattcacct aatgcaatag ctgaattagc tgttactcaa gctatgaacc ttttaagaaa
    29761 gactcctcta gtaaagaaaa aagtctgtga aggtgattac cgttggatag ctgaacttct
    29821 tggaacagaa gttagatcta ttacagttgg tgttataggt acaggaaaaa taggtgctac
    29881 ttctgcaaaa ttattcaaag gcctaggagc taatgtaatt gcatttgacc aatatccaaa
    29941 tagtgattta aatgatatat taacttacaa agattcttta gaagaccttc taaaagaagc
    30001 tgaccttata acattacata ctcctttact tgaaggaaca aaacatatga taaataaaga
    30061 tactctagct ataatgaagg atggagctta catagtaaat actggccgtg gtggtttaat
    30121 taatacaggg gatttaatag aagcactaga gtcaggaaaa attagagctg ctgcccttga
    30181 tacatttgaa actgaaggat tgttcttaaa caaaaaaatg aatcctggag aattaactga
    30241 cccagaaata aataaacttc tttctatgga acaagttata ttcactcatc accttggttt
    30301 cttcactagt acagcgattg aaaatatagt ttattctagt ttaagcagtg ctgtagaagt
    30361 tataaaaaca ggaactgcta ctaatagagt aaattagtat tttataaata tattttttca
    30421 ttaattagct attatctaac aaaatagcct ttaatttccc aaatataaga gagtcagctc
    30481 agtatatatt gagtagactc tcttatatta ttttttatat taaacttgta ctcttcttat
    30541 tcataccaaa ttaaatttat attaacttat tctaactact cctactctat acattttacc
    30601 tatttttact tttctaatta tatacatttg aatgggtaca atctgtattt tacaaaaaaa
    30661 acacctaaca aactatgaaa attgtatgat taatattaat atatttataa gaaaacataa
    30721 caattatgtt agctctggat ttttagggag ggagtatatt ggaaaaaatg aataaagtta
    30781 cttctaaaaa aactacctat attgttactt cagcattatt tgcatccata atatgcctga
    30841 caatagcata tatattacat attccagttg gtggaaacaa tggatatgtt catataggag
    30901 atgcttttat ttatctagct gcaactattt tgccaacaaa ctatgctata gctgcttctg
    30961 caattggtgc tggtctagct gatttatcta caggtgctgc tatctgggta ataccaacta
    31021 taatcataaa accaatactt gtattatttt tcacttctaa aagtgacaag ataataaata
    31081 agagaaatat agttgcttcg gttgtagcag gtatagtagg attagtttta tacatgtttg
    31141 ctgaaggtat aattattgga agttttacaa gtgcatttgt aatgagctta cttggcttat
    31201 tacagccaat aggaagtttt attgttttta taatcttagg tatggcactt gataaacttg
    31261 attttaaaaa acgttatttt aattaagaat ttaaatttat ggagggaaaa ttatgaatat
    31321 cttatataca attgaaaata gtatctatgt aaatataact aatacatgtc catgtagttg
    31381 tgttttttgt ataagaaacg aaaaagatga agttgcaaat agtggtagtt tatggctgga
    31441 acatgagcca agtgtagatg aagtaaaaga agccttcaat aaatacaatt tagacgacta
    31501 tgatgaaatc gtattttgtg gatatggcga accattaatg agaataaatg aattgataga
    31561 ggtagcaaag tttataaaag aaaaaagtag cataaagata agaataaata caaatggtct
    31621 aagtgattta atacataaca aaaaaacagc tattctgtta aaaaatgtta tagatgcagt
    31681 atcaataagt cttaatgccc caaataaaga agcttataat agagttactc aaccaaagtt
    31741 tggagaaaaa tcatttgatt atatgttaga ttttgcaaaa gattgcaaaa aatatataaa
    31801 agaagtagct ttttcagtag tagatgaaat atctccagaa gaaatagaag aatctaaaca
    31861 actggcaaaa aaacttgata taccattaag ggttagacat aagaattagc aagtaaaatt
    31921 ataaaacatt aattataaaa cattaagaat cacctgaatg gtctttcagg tgattcttcc
    31981 tcttgtatta acattttaca aaatagattt cataccacac taggaatgtg taaatcatcc
    32041 aaatctttct ccaattatca gagtttccac taatgtagtc attaaaaatc tcattaatct
    32101 catctatgtt aaagaattta tctgctatct cactattgaa tttatctcgg atgtccttat
    32161 tatattggtc atcagccatc catatacgaa ttggcacaat aaatcctaat ttatttctaa
    32221 aagcaatctc ttctggtaat gtttttgatg cagctgtacg aaaggcaatc ttattctctt
    32281 ttcctttcac tttatattct gatggtattc tggatgcaat atcaaatatt cttgtatctg
    32341 taagaggcat tctgacttcc aatccaactg cagtactcat tttatctata ttaaaataaa
    32401 tatctccttc caaccatatt tgaatatcta catttgacat gttagtaagt gtatctagtt
    32461 cttctgtttc ttcataaatt ccctttacca cttcaatagg aagcacatca ggattgtatt
    32521 tcttaagaat ttttcttttc tcaccctcat tcataatgtt tgtatttcca atataaaatg
    32581 tcttcaaatt ctctccataa cactctgcct tatggtatat attatatcca ccaaaaaact
    32641 catctgctcc ttctccagag taacagatat ttgtgttttc agctgctgcc ttgcatccaa
    32701 tagcaaaaac gatagcagat gcatctccag ttggttgctc catatggtac attacataag
    32761 gtactgacgc aaaatactct tcaggtgtaa ttatatatct cgaattttta cacccaagaa
    32821 tattagctgt ctgttgtgct aattttgatt catcaaaacg ctcttcctca taaccacatg
    32881 aacctgtttt ttctatatct gacattgcca atatataaga tgaatcaaca cctcctgata
    32941 ggaataactc tgcagtttca ttctctgatt ttacctctgg cataattttc tgaagtgttg
    33001 tatgaatttc atccgtccat tcctcaatag acttgctgtg gtcaggatga aattcaggtt
    33061 tccaataacg accaatacat actgttttat tctgatagac aaggtaatgt cctggcataa
    33121 gcttctttac tccacggaag aatgtatttt cacctgctac atatgtgaaa gacatataaa
    33181 tctgtaacat gtcttcattt aactctttca caaatcctgg ttgctccata atcttacgaa
    33241 tagatgaacc atagagaagc ttcccatttt cagtctcata ataatagaaa ggttttactc
    33301 caaactggtc tcttaggcag aagagtttct tttccacagt atcccataat gcaaaagcaa
    33361 acattccatg catgtgattt gccatatcat atccccatgt cttatatgct tccactaaaa
    33421 ttgctcgttc acgctcatct ctacataatg aagaatcgat tccaagttct tcacagagaa
    33481 ctttccagtt tcgaatatgt ccattgtatt ctagaatttt tatcatatat gtgtcttttc
    33541 gtgttaaata cttttcttct aaatttatca tatgtgtatc ctttcgtatt aaaacattta
    33601 acttctaaat ttattaaatg tttatatata tttattcact tataatcact gaaaaatcga
    33661 cttttttcaa agtaaattct ttttgaatta gtactaatct gctctttatt aatggcatca
    33721 atatgattat caagtattat cagtgttata acactgaatt ttttattctt cataaatatt
    33781 gctcccttta tattaccaac taaatataat atatcttatg ttacttacat attctttaag
    33841 aaattctgaa gattttataa agttttttgt attataatca attaaaaata aaattctagt
    33901 tataaagcat attttccatt aaacaaaaaa atagttaaaa taaattcata actacataaa
    33961 cttattttaa ctatttgtat attttaatta taaatattaa cttaaatttt taacaggata
    34021 cactcataaa ataaacataa tgaacaacaa aataatatgt ctcagtttct ttatacactt
    34081 taccttcttt caattttcta ccatgtagac gtacaagata atattagtaa ttcactctat
    34141 tgctgtaatt tactctattg ctgtaattca ctctattgct taactcgtgc gatatggaca
    34201 accaagaaag ctatttcctc atttgttaac ttttttccat atttcttttc taaaaaatct
    34261 ctaactttta atgcaattaa catttctttt ggatttgacc ttttcatttg attaaataat
    34321 aaatcatcac cactgcttaa catactatct gaaaaaaatc tttccacaaa aaattttata
    34381 tgtgttataa atctcatata atgcatactc tttttatcta aggttctatt caaagaaaaa
    34441 acaaaaatat taattatttc tccaattact ttggcatact taatcgtatc atatgaattt
    34501 ccatctgaag ccatagcatt tgcaaaatgg aaagcaatgt tagctgcttc ttcctctggt
    34561 aattgaatgc ccaatctctc ctctaccata taaattgcaa ccattccaac cttaaactca
    34621 tttggataat aattttttat ttcccaaaat actcgatttg taatttttat tcctttacgc
    34681 atcctttcaa ttgcaaagtt aaaatggtct gctaatatta agtataagtt tttattaaaa
    34741 gatgagttca ataacttttc tgcctctact attatttcct gtgttatctt taaaatgtcg
    34801 acaggaatat catccattaa ttctaaaaat tgttttgatt ttgtattttc aacagggaca
    34861 aacatttgat tatcattgga accacaaata tattctcctg tttttttacc atatccaatt
    34921 cctttcccta gcaaaataaa ttcattatcc tttgtgtcat gaactaaaac aacgctcgag
    34981 ttcaaaactt tctttattat gtagttcatt ttatttgctc accttctatt ggtcatttaa
    35041 aatctatact attattttca attacttttt tataccaata aaaactatct ttcttatatc
    35101 gattatatgt cccctttcca taatcatcag catccacata aatatatcca taacgttttg
    35161 acatttgttt cgttgattca ctgaccaaat caatacaacc ccatgaagtg tatccccaaa
    35221 gttcaacgcc atcttcgata atcgcatcta acatacattt aaagtgttct tttaaataat
    35281 taattctata ttggtcatga acttttttat cttcagttaa aatatcttta gctcctaatc
    35341 cattttcaac tataaataaa ggctttctgt atctatcata taattcaatc aaagatatac
    35401 gtagacctat tgaatcaatt tgccaacccc attcactcat ttctaaataa ggatttttta
    35461 cagctaataa cgtattccca ggtgtaatat ctaatccagt tgtatcagct gcctcacaag
    35521 aagacatata gtaagagaat gaaataaaat ctactggata ctttttcata atttcatcat
    35581 catgttcttc ttttactatt tgaatgttat tatttttaaa atacgacagt aaatatactg
    35641 gatactcacc aaaaacctgc gtatcactat atgcataaat acttctcatt ttttgttgag
    35701 ttgccaaaac atcttctggt ctacatgtat atggataata cgttaatttt gttaacatac
    35761 accctacttt tgaatttgga ataatctcat ggcaaatttt tgttgcaagg gcactagcta
    35821 caaattgatg atgcatagct tgaaaaataa cttcctcaaa gtttttatcc ttaaaacggt
    35881 ctcgaactaa accaccagta gtgtacggat gacgaatcat agaatcaact tcattaaatg
    35941 taagccaata tttcactttg tttttatata tttcacagat agtttctaca tacctaacaa
    36001 acatattgat aacttgtcgt gaataccatc catcatagtt taaaacgata tttaatggtg
    36061 gttcataatg tgacatagta acaagtggtt caattccata acttatcaac tcatcaaata
    36121 catcatcata aaactgtaaa ccttcttcat ttggctcttt atcatctcca tttggaaaaa
    36181 tacgagacca agcaatactc attcgatatg tcttaaatcc catttctgcc attagagcaa
    36241 tatcttcttt atagtgatga taaaaatcac taccatgacg ttttggataa tttacttcat
    36301 cttcaggatg tgctaatcct tcttctatat cttttgttgt gatttcatta ttagatttat
    36361 aatctttcac atctgcatca aagtgtgcct ttgctacatc tgatacagac caccctttcc
    36421 cacctaaatt ccaaccacct tcaaattgat tagcagctgt agctcctccc caaagaaatc
    36481 cttctggaaa ctttctcata tttatattcc tccctttaca tcaaacaaga tacttccttt
    36541 ttttacattg ccataagact ggtttaacac tttataagca taatctgctc cattattgtt
    36601 tacaatcata acagttgaat ctaaattagc atgtgagatt ttgtctaaat caaattgaat
    36661 taataaatct ccttctttta cattatcgcc tgtcttaaca aatgttttaa atccatctcc
    36721 attcatactt acagtattta ttccaatatg aatcaaaatc tcagccccat tttttgttct
    36781 taaaccaatt gcatgttttg tttcaaataa catcacaact tctgaatcac aaggtgcgta
    36841 taaatttcca ttggtcggta taattgcaac tccatctccc aaagtcttac ttgcaaatag
    36901 ttcatcatta accttactta aatctattac ttttccttct actggagtta ctaagatatt
    36961 tacttctttt tcttcttcta gctcttcttc aacaacatct ccaattgatg actctactgt
    37021 atttgtatct tccttccaaa taatcagtgt aataataaac gagcctaata ttgcaataac
    37081 aaaaccaata cttgcaaaaa tcaaattgtt tccattgttt gggtcaataa acatactcat
    37141 actggcaagc cctggtccaa caattacaaa tccttttact gcaaataatc ctaaaaatgt
    37201 accagaaatt gcacttgaga atacaacacc taacattgct cttttatgtt gtaaagtaat
    37261 accatataaa gctggctctg taattcccat caaggcagaa attcctgcag ataatgctgt
    37321 ttgttttaat gtttcatctt ttgttcgcaa ggctactgca aaacaagcac cactttcaga
    37381 aatattgtga gctaatgaag atgtcatata taaagcttca taacccaact ttccaattgt
    37441 attaattgaa taaggtatta gtgctttgtg cattcctgtt gcaaccataa atggaagaaa
    37501 agcagcaagt aatcctacaa ctaagaatcc cattttgtta tacaagaata aaataatggt
    37561 tgtaaaaatt gtaccaacgt tatatcctaa aggacctaaa atagttagag taattggaac
    37621 agtaattgcc aaagacatca taggaacaaa gaaagttcga attggttttg gtgaatactt
    37681 gttaaaaatt ttctccattg ttgccaagaa agacacacat aaaatagctg ggaaaacctg
    37741 tgcattatag gcaatgtttg gaattgtaaa tccaaatagt tcgacacctt cacttaaagc
    37801 agttgttgtt gctggaagca ataagtatcc tacagcggca atagctacta aacgattaca
    37861 tttcaataca ttggcagtag ttacagctac cataagaggt aggaaataga atgttgcatc
    37921 tgaaatagca actaataatg tatatatagt agaatctgat tttaaaatat taaacattgc
    37981 taataacatc aacagtgatt ttaaaatacc agctcctgca atagcaaaaa tcagtggttg
    38041 aaaaatatta ataataaatt ctataaatct tgttcctaag tttatttttt cttcagtttt
    38101 tacagtacta tcatttgtat ctaaagggca aacttttaaa atttcatcat acacatcaac
    38161 aacactattt ccaatgacaa cttgaaattg tgccccatta actatactca taacaccttg
    38221 tacttttttt aaagcatcct gattgacttt acttctatca atcaacttaa agcgtagtct
    38281 agttgaacag tgttctaaag ctaaaatatt ttttgaacca ccaacttgtt caataatgtc
    38341 aatggcagtt tttttataat tcatcttgta atctccttct ccataatttt ttatgaatgg
    38401 ccattcaaga tgcaaaaaag gcagaaccta tataaacacc ttgaaaaatc aaagcaatat
    38461 attcgatcct gcctgcatta ccagtaacat gtcttctatt tatactattt tgtattatta
    38521 tattaacatt tgcctaaaag aattgtcaat acttttttaa attttctgat actaactaat
    38581 agcaccttta taattcattt tttatgtcaa ttaaagtaac agtagccacc caccaattag
    38641 tatcaaaatt gaacctggta ttttttcttt tatattttct ttgaatataa aatgagcaag
    38701 tattaaagtt attatcatag atacttgaga aaatggctct gctatactaa gttctagatt
    38761 atttaaagca attaataatg ctaaataaga atatccatta atagcaccac ttaaaattga
    38821 aagtcccttt ttattagtaa aaatctcaca aattattttt tgtttctttt taaatgctaa
    38881 aaagataaat aaatttaaac ctacgaaaaa atacagtata gttgaatata atataggaga
    38941 tacttgtaca aaaaaagcct tatccaatac tcttcctact gactgcaaaa atatgtataa
    39001 aaacattaat ctacatggta aattgttaaa aattacagag acattcttta aaaaagagct
    39061 tacttttttt aatataaaca ctccaccaat cataattatg attcctgcaa cttttgctaa
    39121 acttaaacct tcacctaaaa atataactga taaaagcagt aaaaacaatg aatttaaact
    39181 atttattgga gtaacaagtg aaacttcccc agttgctaaa gacgttacat acgctactga
    39241 accaattgaa tagactaaac tacttatata acatggtatt aaaaacgacc aatcactaac
    39301 tctatccatt atgctaaatg gaagtaaaca tactccccct ataaagaaaa atagaaatga
    39361 tgaagctata tttttataaa tatctccttc ttcatcacct aataatctta caactactct
    39421 ttcaaaagct aatagcacta ctctaattaa taatgcaaaa taagataata tcattttgac
    39481 ctcgcttaaa aattaatccg acttattgtt atattaactg tataaaacta ccatataggt
    39541 aagtcttttg gattttttaa tattttatgt ttaaatacag gtacattaag tttttcacct
    39601 agtttacggc attgctcttt ataggtacct tttaatttat catgaaaaac tatacaatca
    39661 attcctcctt caatactatt tatacacttt tctagtttct caaatccaga ttcatcatca
    39721 tacattccac ttacaatcca ttgatttggc aatacatgtc caattgatat gttatattct
    39781 ttatttttta taatatctga gaaagatttc tcaaaatgta cccctccaac acataacaaa
    39841 gtcttcaaag gttcatcaca atcaaatact ctaattaatg actttgccaa tacctgtatt
    39901 gctatactat tattaaaact atcactacta cttcctatct caatatccaa cataggtaca
    39961 gaatattctg taattaattt agattcttca ccatatgttg ttccagacca gtgagttgct
    40021 tctgttaatg tagtaaattc atcaagtaga ttttcatttt ttatatcctc aatagcattg
    40081 attagatttt taaagtatct tggatttgaa tttccaaatt ctcctgttgg aacatctcca
    40141 attgtatgag ctgttaggat atgttctgga gcattttctc cctcatgcca atttacaact
    40201 cctgcaaaat cataatctct gaaatgtcta tttaaaattg gaagataata tttataatta
    40261 tgacttatta catcttttgt ttttacaaaa tgaaataagt tattgttttt atccttaaac
    40321 tctaatacaa aattatcatc aataacaata tcagtttgat ttaaatccat tatttcttca
    40381 caacacttta tcacattact ggctactggg tctttcttta aatcactgca aaagaaaaaa
    40441 actgcttttt tattattcat ccataaatca cactcctata acttaaatat aatatatatt
    40501 catacctaaa atcatctaaa tatcactcta attccattaa attatttata tataatatac
    40561 taccatactc attttagcta tacctttgcc tgagtttcta tatatatgtg atacatcagc
    40621 tttaaatctt atagaatttc cactagatac aatgtaatct tctccattta ttttaatggt
    40681 tacttcgcca ctaaaaacag ttataaactc ttgtgttcct ggaagttcag catttgaatc
    40741 taaatttcca ccttcttcta tttcaatcat aaatatctca aatcttcttt tggagtcaaa
    40801 tggaaatatt ggataactta tatactttcc attatcttca ataaaatggt ctatatcact
    40861 tttttgaatc aattctatgt cactttcctg cctctccatc aatgatgtaa atgagatttt
    40921 tagcccatta gttattttct tcaaagtaga tatagtagga tttacctcac acttttcaat
    40981 ttgagatagc atactcttac ttacaccact aagttgtgca atcttgtcta aactaagatg
    41041 tttttctttc cttatctttt ttaaattttc agcgattatt tcatttaaac tttgcataaa
    41101 taccccctaa tacatactaa tataagataa attattttga ttattaaata aacttagtaa
    41161 tttaatagaa actttttatc cattataatg tacattatgt ttaatataca ttactttttt
    41221 tagaatgtaa atagttttct gaaattttgt gaaaatgaat aaataaaaaa ctagtaacct
    41281 atatttttac aaggttacta gcttttatta taataatctt tcattttaac tataaaactt
    41341 agatgcacct ataagctgtc tcttagaaac aatcttagct aggtttgcta atgaagaaat
    41401 ttttatatct ttaaaagaaa ctaaaaatac aaacttaaaa tctgtaaatt cttgatttat
    41461 attgtatata tcaaaataac atctcaaatt atcaaataag taattatcct gtaagtctgc
    41521 ttctatctca tcagtagcat cttttaaata cgctcttaca tcttcaaata aaacactgaa
    41581 ataagactca tctatttctt cacctaactc ttccaatatt tcttctatat ctaaatcatc
    41641 catatcaaaa gaatttcctg tcaactggca atcaaatacc aatatatttt cttgatttat
    41701 agtatttgat atatcttgta catattcgaa gtctggacgt aattctaaaa tgtcttttat
    41761 ttcactgctc tttacatgga tttttccttt tctattttca atttttaaat ctatcatatg
    41821 ctcccccttt caatctacaa atctttaaat accttgtttt ataatctttt caatatctat
    41881 ccaattacta actctaacta tattttgatg tttaacatct ttattatagt tagtatccag
    41941 aagtattact ttaacaccat catcagctag ttgcactgag tttaaaggat tatcttctat
    42001 aaatatgtca caactcagtt ctcttgcctt ttcaacttta taatcacttc ctaataagta
    42061 aacatctata tctgaaaatc cttgtcttga tagccaattt ttagttacat cagttaaaca
    42121 ctcatgtcta gccgttacaa aagataaatt atgtccttta gataatgtgc taattacctc
    42181 tgtagcaccc tctacaatct ttgcttcatc atatgagtgt atatacttat tattaaactc
    42241 cgcatacaca tctcttattc ccgaaccata caattcttcc caattatgtg ttatacactc
    42301 tgcttcagtt atatttttat taaagatatc atttaggtat ggaagaaaat gataagggct
    42361 tgttattgtt ccatctatat caatacatat attcaatttg ttcattttta cctccatgta
    42421 acatttattg atttgattaa tcaaatatta tagcacagct ttaattataa atcaaaactc
    42481 tggatatgac tatacctata taataccaaa aagcttgcat atttaagcaa gcttttcgta
    42541 aattttacat ttttaactaa tatataagtt tttctaaggt tggttccact ccaaattgtg
    42601 atttcagttc tttatattct tttaatgctt tgtcattatt cgttttagtt tttatcgctc
    42661 ttatcattaa attttttggt gtatcaagtg gagatatgta ttctactaca gaagtatcat
    42721 atccattgcc ctccaataac aatgttctta aaccatcagt tattaaatct gcaaatctag
    42781 ctttaaatac accatgtttt aatattggtt ccatagcttc atatctatat tgccctaata
    42841 actccttatg acaacatggt actactacta tagcttcaga ttttgctcta attcctagac
    42901 ctattgccat atctgtagct gtatcacaag catgtagact tattacaagg tcaacatccc
    42961 tattaggtgt ataattagtc aaatcctctt ttataaatga catattttta taacccaagt
    43021 ttttagccat attttttgat gcttctataa ctacatctga ataatctatt cctataaaat
    43081 aacagttttt cttcaatact tcttttatat aaaaatttaa gacaaagctt aagtaagatt
    43141 ttccacaagc acaatccaag atagttatac agtctttatc ctttatttct tttaaaatac
    43201 tatctatcaa ttcaacaaaa tggtctatct gattgtattt tcttatttta tcatttttta
    43261 tcttaccatc ttttgtaagt actccaattt cttttaaaag tgcatttgct tgtcctactt
    43321 ttacataata atctctgttt tttatttgag atgccgtaaa ttcatctatt ttaggtactt
    43381 cttccttatt gtctaaatat tttacattta ccttcttatt gtctccttca attacaacaa
    43441 tagttcctcg ctcaacatat ttaaatacta atccatcata attttctgcc tctttagaaa
    43501 tattcttaag caactcatta aacgtatata aatctgattt tcctttaaaa ttaaatttat
    43561 atttattatc ttcctctatt gcaattcctt taaactcttt tcttccagat ttaaaaatca
    43621 cctctaactc aataaaaaaa tccctattat caaggtttct ttgagtaatc cccataaaaa
    43681 acatatttag ttttcctata ctctgtttat tcatcataat ctccctttta acttatatcc
    43741 ttaaatctta ttaattaata ttatttgttt ctgaattatt tatttcttct tttaaatcgt
    43801 tcaatctatt tttaaaactt tctatctgct tatctgataa atcaagatat tctgttatct
    43861 tccctatata tttagggctt ttagtattta ttaatttcaa agcactttcc atagattttt
    43921 ctctaagctc atatgctttc tttacgtcat ctctagcatt actcaatgtt tctgtatact
    43981 tctccttaga aagttctgga atctgcatat tttcatattc ttttacaact tctttacact
    44041 tgtttctggc atttgtaaca tcttttttta tactagataa tttagttata ttttttgcta
    44101 tatccttagt gtaaggtata cattctctca ttggaactaa agctgctctt tctatattat
    44161 aaatttcttt cttaaaaatt cctatacttt ccatatctat tttttttata tcttcactat
    44221 ttttttcttt acttgtatat ttattatttt cttttgtatt atcttcttgt ttttctgatt
    44281 tattattcac tgaaacatta ctatcagaat tattcttttc ttttatatca ttacttcctt
    44341 cttcattact ttctttattt tgtatatttc tatcttgttt gttgtttaaa tttttattat
    44401 ttgatatttc ttcattcttt ttattatcct gttttaaatc agtactttct ttttccttaa
    44461 aatttgatac atacttttca tcatatgtct catcagaaat ataattgtga taatacatag
    44521 actcttttat tttattaagt ccactcatat aaatattact tatcattatt gttgtagcta
    44581 ataccacata aatactactg tagatacaag acctcttaaa attcttgtaa tttctgaata
    44641 tcactactaa aggaataaga tataaaaata taaaaactaa tatccacctc ataagtcgtg
    44701 tctcctttct caaaaccgaa atccctcaaa tatttatttt taatcatgta tcttatagta
    44761 tttaccaatt tatgtttttt aatcgtgaac ttttattaaa ttaaattctg tatagtcaca
    44821 agatgctaat gtatattcta ctccatttcg aatccccttc aatcccattt taaaagattc
    44881 tttaccatgc aagtgaccat aaatcacctt ttcaacatta tattcttcaa ataactttgt
    44941 aaataaagat tcttccaatt tatcattcgt aggaggataa tgagtaatca caattatttt
    45001 tgagtgacca cttttacgag cagattctaa tgatagtcta agtctatgtt cttctctttt
    45061 ataaactttt tcatcagtct catcaaattt aacatcatta ggacatatcc agcccctacc
    45121 tccacaaatt gcataatctt tatattcata aaaatttgtc tgaataaaac gcatgtcttc
    45181 atatagctta tttaaacttg taacggttgt ccaccagtaa tcatgatttc cctttataag
    45241 tattttctga cctggaagtt tgcttattat atctaaatct tttttagctt cggataaatt
    45301 aattccccaa gatgtatctc ctagtactaa gaccatatct tcttcctcaa caacttcttt
    45361 ccaattatct ataattttct tttcatgacc atcccaatta cttccaaaga tattcattgg
    45421 cttattgact gatgttgaaa aatgtaaatc acctatagca tacaaactca taatatcatt
    45481 cctttcttaa ttacactaaa taggctgtct ctttttagaa attattttcc attaagaggc
    45541 agccccatta atatttagca aattttctac aagtagtttt tactgaacta ctttatcatc
    45601 taccttagta tccttatatt gctctaataa tatatctatt tcttgtttta attccaaaat
    45661 ttcagttata tttttatctg tttcagttct atcaagtttt ctttggtact gcatagcttt
    45721 atgtgtatca cccaagtcat ataactcttg tattctattt agagtatctc taatagctat
    45781 agacttcatt tggtacatag cctgagcttt cattatgtcc tctcttattt cactcatttc
    45841 ttggtctaaa agaaaggcag catcagatgc tcttctaagc ctttcagcaa gagtctcagg
    45901 tatgtaatgg tcatatttat attttaatga atgttctatt gtagcccaga aattcattgc
    45961 aagagttctt atttgtattt cacatataat ttcctttgaa cccgcaatgg agtttatagg
    46021 gtattttatt ataacatgat agcttctata accactatct ttgtaatttg taatataatc
    46081 tttttctcct attatagtca tatcatttct tacttttatc aaatcaacta tagcatatat
    46141 atcctcaaca aactggcaca ttattcttat accagcaata tcatcaattt cagtttctat
    46201 atcttttgca tttatccttt ttaacttaga tattatggaa gatatttttt ttgttctacc
    46261 tgtgacaaac tctatgggag aatattcacc ttttgctaaa tattcttttc ttatattttt
    46321 aaattttatt ttcaattcct ctactgcatg ttcataaggt gctagtatct cattccattt
    46381 ttcgtattcc atattgctca ccctttattt gttttttatg accttcttta ttttatcata
    46441 atttattttg tttttgtata gtaaagcaat tctccatttt ccaatatata ccctatttct
    46501 tctaaatcaa ctaagtaact tatgacagat actaacgaag atttaaaaaa atgatactct
    46561 ttataattat tgcttaaatt attgttattt ataatgtttt ttaataaaat ttctaaagat
    46621 ttcggttcta caagcaactc tcttatttga tataaatatt tttttaaagc attcaaatgt
    46681 ttttctatta atttataaga atcttctttc gatataactt gcttgccatg ccctaaaact
    46741 aagtactcaa aatctatatt ttttaatttt tctaatgaat ttatttgttc ttgtatatca
    46801 tatagaaaca agaaatcgta ttttgataaa atttcatctc ctatcaataa atcgccaaca
    46861 aaaatcacat tatccttcgt caaaacagct atactaccag gtgtatgacc ctgaaattca
    46921 attatatcaa atatttcatt gtttaattca atttttccag cttctataga tttatctatt
    46981 tttatatcat ccaacatttt attttttaag atattatcca taaatacatt gctctttccc
    47041 ccaacaatat actttgaaaa caattctgga ttttctatat ataatttatc gtattcagat
    47101 gacattatac acacatcttt ataatattct ttaaattgat tacaagctcc atagtggtca
    47161 tcatgaccat gagtatttat aatatatcta acctgtatat tattttcatc taatacttta
    47221 tttactctcc taggtcttga accacataac cctgggtcta tcataagtgc tgtgttatct
    47281 tcaaatacat atattcctgt attcgtgcca ccttttataa aaaaggtatt tccttttact
    47341 tttgtaagat tcaccttctt cacctcattt tgacaatatt tatgatacaa tttattactt
    47401 actaatataa aactatacaa gaacaatcct ttttattcta tctatagttc tttttcttat
    47461 tttgtattct ttagatatcc ttattttcac attatatgct aaatttctat aaagtaatga
    47521 ctctataaaa tattcaacta tacctctatc cctaaagtct atatctatat ttccacttga
    47581 ataaatattt gagtctaata tcataagctc atcaaaaaag cttaatctct tagatttact
    47641 taaataatct ttatttggaa taaatctatc acaataatct atcctgtaat ttactacttc
    47701 atcatttatt tcaaatccat attgcttttt cattaactct ctatagttat tatttttctc
    47761 aataacatta tctaaatttt tattataatc ctcttttttc caatgtccaa acatcctaca
    47821 atttaatggt ctaacttcat atattaaaca tctattatta gtatctttaa atggacatgg
    47881 gttctttctt gtatactcct caaaataata atctaatatt ttacttatac agcttttttt
    47941 taaatcaact ctatcttcaa gataacaaaa tatattcaaa aactcaatta aatttatacc
    48001 tactgactcc atacaacaat ttccacatcc agaacaattt cctactggca aggacttata
    48061 tacatcattt agtttgtcaa agagcttatt tttaatggaa taatctatac aatttaaaat
    48121 atctttattt tttatactag tcattattat tactccttgt tataattatt aagttaatac
    48181 aaacaattat tataacatat tttttaaatt taatcgcata tggaggtatt ttatgaatag
    48241 atatacaaaa ataattaata tgatgggaag ttattacaca aaagactttg aaaaagaaaa
    48301 aaagaatgta ataaaagtta gagaagttaa agaagataca gttagaaaat ttttcttaca
    48361 aggtgactgt gaagttcttg tagtatttga agacactggg aaagaaatac ttattgatga
    48421 cttttctcct gaagaagata tcaaaaaata tttaggagca aaatttataa ataaaaaaag
    48481 atagttatca aaaataattc taaaattaaa atttaataaa tgtacagtta taattctata
    48541 taattatagt taaaagttat cttttattgc ttttttagac tttaagatag ctttttttct
    48601 attttttgta aaataattcc ataattcaca ttaataattc acaaaatgat ataatataac
    48661 aaatagataa attttaaatt tctaaataca taattaatat tttgagaggt gattttatgc
    48721 aaatttattt tagagttaag gctgttggca aacgcaaacc tatgctagaa cttacccctt
    48781 ttgatgtatc tgacaacatc aattgcctaa aagaagctat ctctgaaatt gtatctaaaa
    48841 atgttcttga atataatgag aaaatcccag aaaaatcaat aataaacttc ttaactaatg
    48901 aagaaattga atctcaagct gatattggaa aagttggatt tggctctatt tacaatgaga
    48961 ataaacaatc tgtagaaaag gcaatagaag tagcgttaca ggcatttgaa gatggaatct
    49021 acaaagtgct tataaatgaa gatgttattg aaaaactcga ttcctctata aatctgaaaa
    49081 gtggagatat atttactttt ataagactta ccttgcttgc tggaagaatg ttttaaaata
    49141 aataatttgg aggtacataa atgttagaac gtactagagt agaaaaaaat cctaaaataa
    49201 ttgaaaattt taaaaataat tttactaaat cttcttcaaa acagtactac gacttagcaa
    49261 agtctctttg ggattgcata gaaaatagtt catactatta tagagaacct gaagacaaat
    49321 tcttaaatat gataaagaaa tactacaatt gccactacta cgaaattaaa aatatggacg
    49381 agctcctttc tggaccttta aaaaatgcat tagaattaat atttacttct gaacaagtag
    49441 aagaactttg ccaattaatt aaaagaaaga tggattatac atattcagga ggatattatc
    49501 gtaaaggtta tcgctcaaat aaacttgtgg attattttag aggtattata aggtttattt
    49561 caaactatgt tgatttaatt tattatgatt tttcagttat agattatctt actcttccaa
    49621 ataaggtctt gtactatgac tctataatga gtgataaaat agcactagaa atagacaaag
    49681 gaaatcaaaa agtaataagt gctttagaag aagtaatatt tggagaaaat caaactgctc
    49741 tacttacaag acaaatgata attggtatca tgaagagtca tagtgataaa tgctatgaaa
    49801 tgctttctaa actccttttg gcagccaaac aacaagaagg acttagacag caaattgtag
    49861 agagtttaga tgagggtaca atttctgcat caatatacat gttaaaaact attttggaca
    49921 acaatttaga gcgatttagt tctgttataa gagccttgga tacatggaca ggacttggtt
    49981 tttcagacca aaaacctgga ataatacgta aatgtttaga actttcatat aaatcattga
    50041 ctgatgaaaa gttccgtata gaatcaatta atagcaatga taatcttgaa atttattttg
    50101 ccttgtgggc tactgcaact tacaacatat atgactctta ctctcttatt gaaaacttac
    50161 tttcttgtca ggaaaaatat aaaaaacttg tagctcttta ctttttatcg catacagaaa
    50221 gtacatattt taaacattct atagcatcta aaaacattaa gggtgatgat ttagagatac
    50281 tagcatgggt aattacaaat ttctatgtag aaggttctgt tggctactac tatagttggc
    50341 aaaacagaga aactaatttt gacttgtcaa acactcctat tgacaaagac tttgatgcta
    50401 gaaaagaaca atttgaaagt ttaaaacata ctttagaaat tttaaattca aagacaaaat
    50461 cattttcagg aagtgtattt ccatggactt ggattgaaat ttcttctcat gaaatactta
    50521 aatgtatgat gactttaaca gcatacgata tgaatgctca atttatagat gtgcttatgg
    50581 aaaattgtca tcttatgagt tcagatttaa gagcttcact tatagtcaat tttgtaaatg
    50641 aaccaaagac agatagacaa cgtgaatttt tattggaatg tttaagtgat aagagtgttt
    50701 ccaatagaga aacggtattg agtctactta gaaagcttga gctaacagat gtagaattag
    50761 ataagataac caatctattg tctttaaaga caagctctat tagacaaaca gtaataaaaa
    50821 ttttacttga acaagatgat tcaaacttgg aaaatattgc ttcttcactg ataaaatctt
    50881 caaattctca aaaatgcctt ggaggtcttg aaattgtaaa cttattaaaa gacaatactg
    50941 ctagacaaga actatataat aagcttgttt tacttataga agatattgat aaatctaaaa
    51001 agaatacatc tcaagttcaa atactaattg ataatttgtt aggtaaagca aaaaatgatt
    51061 actgcaaaga aaatggtttt ggaatatata accatgaaaa caaagttcaa attccagaac
    51121 aatttgattt taaagatgat tctaatagct catcaaagct taataaaatt atttcaaata
    51181 taagtttatc tactcgtgag gattctgttt ctgtagcaac aaaaaaagaa gcaaaaaaaa
    51241 ttctcgatac ggatataaat aaaataataa aagtttatga aaaatttgat gagcttatta
    51301 ctgaaaatgc agattatgaa tatgaagttg aaagatggtc aggtaatgta gacaaagtac
    51361 ttttaggtta ttctacttat aacttgactc ctattaaata taaagtttct aatgatactc
    51421 ttgataatta tccattagca gaactttgga gagaaactag taaaaaactt gacttagatt
    51481 acaaattaat acttgatatc ttattttata gtataagtac atgtggatat aatgaaacag
    51541 aaaaaagttg gttttcatct atatattcaa atgtccttga ctctaaaaaa caaaataaaa
    51601 tatatacaaa acttgaaaaa cttaaattta atggacttac aagaaaactt ttaagagttt
    51661 tactaaatga gtttgaccat aaagaaatat ttaactattg tttagaaata tactataaac
    51721 ttagaaactt attgactata gaacaatgta aaaaacaaag agttgaattt actaaaaact
    51781 attacagaca aaatgaatac tgccctgctt taaatgcccc tattttaaac tactggcttg
    51841 ataaagcaca ttcgtttatt tatgatgata tttcttttac taagtttttc tacactacgt
    51901 tttcaaatta tcagttgcta gaatataagt ttcctttcta tactagcata aatgtgtttg
    51961 ctcgtgcaca tgaattagga ttaatgactg aagagcaagt taatagagaa atgataagtg
    52021 gatacaacgc atcaaatata ataagaacat tgacaaatcc tagatgggct ggcgacacat
    52081 tagaaaagta cccttggctt aaagataatt taaaaactgt tacaaaccga attatagaaa
    52141 ttgaatcacg tagaggtgat atgccaacag aagtgtctca tctagctaat aatatacaat
    52201 actttgaagg tataaattat tttgtaaata ttttggtcgc tcttggtaaa gaaactttcc
    52261 agagaggata ttcatatggt agcaattatt caaaaaaaga tgttttaagt tctctactta
    52321 aaaattgcta tcctactaaa gaagatgatt caaaaaaatt aaaaagttta cttaaaaata
    52381 ccgacattac tgagttaaga ttgatagaat ctgctatgta tgcccctcaa tgggtaaaca
    52441 taattgaaaa atatttaaac tggaagggtc tgaaaagtac aatttggtac ttccatgcac
    52501 atataaatga aggcttctct gcacaaaaag aatcagaggt tgctctattc tcccctattt
    52561 caccacgtag tttcattgat ggtgcatttg atgtagattg gtttttagat tcatataaaa
    52621 ctattgggga agaaagattc aatatacttt acaagtcagc taaatatata actagtggtg
    52681 gtaactctca tagacgtgca caactctatg ctgatgctac acttggtaag cttgataaag
    52741 acatgttgat gaaagaaatt gaaacaaata gaaatcaaga taaattgaga agtttctctt
    52801 tacttcctat gaaaaaagga gacctaaaag aagcaacttt ccgttatgaa tttattcaaa
    52861 agtttgctaa agaaagcaag caatttggtg cacaacgtcg tgaaagtgaa tcaaaagctt
    52921 gtactatagc gctagaaaat ctagctagaa gtgctggatt tggagatgta aatcgtatga
    52981 tgtgggctct tgagactgaa aaactaaatg aagttgctca tctatttaaa ccaaaagata
    53041 ttgatggtac ttcaatttgg atagatgttg atgaaaacgg aaaagcatct actaaagttc
    53101 aaaaaggcaa caagtcttta aagagtatac caaaagctat tgctaaacat ccatatgtac
    53161 ttgaacttaa atctgtccaa aaagatttaa agagtcagta ttctcgtgca aaacagagtt
    53221 tagaacaagc tatgaaaaat gaaatagtat ttacagttaa agaaattgcc aatatctata
    53281 aaaatccagt tatctgccct ttgattaaaa atctagtatg ggtatgtgat gataatattg
    53341 gatatgttta taatgataag tctaagttct ttatttgcaa tatagaaggt gaaacttata
    53401 agttgacggc aaaaaataaa ttaaaacttg cacatccagt tcatttgttt gaatctggta
    53461 agtggagtga gttccaacat gatttattta gtaagcaaat tgttcaacca tttaagcaag
    53521 tattccgtga gtattataga ataaacagtg atgaattatc tgaaaaaact atatctcgca
    53581 gatatgctgg tcaccaaatt cagccaaaga aaactgtagc acttcttaga aatcagcttt
    53641 ggactgtcga ttatgaagag ggattacaga aagtatttta taaagaaaat atagtggcaa
    53701 agatgtttgc acttgcagat tggttttcac cagcagatat agagcctcct actattgaaa
    53761 cagtagaatt ttttgataga aaaacttata aaagtttaga attagcttct atacctccaa
    53821 ttctattctc agaagttatg cgtgatatag accttgttgt aagtgtcgct catgctggtg
    53881 gtgttgaccc tgaaactagc cattctactg tagagatgcg tatagcaatt gctaaagaac
    53941 ttttattatt attaaaaatt aacaatgtca attttgaagg ctctcatgct cttatagcag
    54001 gtaatttagg tgagtattct gtgcatatgg gttctggtat tgtacataaa aaagggatgg
    54061 gtgctttgaa tatacttcct atacattctc aaagtagagg taagatattc cttccattcg
    54121 ctgatgaaga ccctaaaact gctgagatta tgtctaagat actattactt gctgaagata
    54181 gtaagattaa agaccctagt atcttaagta atttataagt ataaaaacta tctctaaaat
    54241 atattggact ttcttaaaaa aattag
//
LOCUS       DAENCV010000009        53540 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAENCV010000009 DAENCV010000000
VERSION     DAENCV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.009"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            107..1297
                     /locus_tag="KQD82_000488"
     CDS             107..1297
                     /locus_tag="KQD82_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4378992.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1467..2429
                     /locus_tag="KQD82_000489"
     CDS             1467..2429
                     /locus_tag="KQD82_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG4378993.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVVGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2551..3213)
                     /gene="zmp1"
                     /locus_tag="KQD82_000490"
     CDS             complement(2551..3213)
                     /gene="zmp1"
                     /locus_tag="KQD82_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG4378994.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3313..3398)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3584..6502)
                     /locus_tag="KQD82_000491"
     CDS             complement(3584..6502)
                     /locus_tag="KQD82_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG4378995.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPMVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6620..6705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6832..8010)
                     /locus_tag="KQD82_000492"
     CDS             complement(6832..8010)
                     /locus_tag="KQD82_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG4378996.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8385..8570
                     /locus_tag="KQD82_000493"
     CDS             8385..8570
                     /locus_tag="KQD82_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG4378997.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKKAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8635..11409)
                     /locus_tag="KQD82_000494"
     CDS             complement(8635..11409)
                     /locus_tag="KQD82_000494"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG4378998.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11581..12837)
                     /locus_tag="KQD82_000495"
     CDS             complement(11581..12837)
                     /locus_tag="KQD82_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG4378999.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12909..14411)
                     /locus_tag="KQD82_000496"
     CDS             complement(12909..14411)
                     /locus_tag="KQD82_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG4379000.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14502..15692)
                     /locus_tag="KQD82_000497"
     CDS             complement(14502..15692)
                     /locus_tag="KQD82_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4379001.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(15931..17019)
                     /locus_tag="KQD82_000498"
     CDS             complement(15931..17019)
                     /locus_tag="KQD82_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379002.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(17304..18002)
                     /locus_tag="KQD82_000499"
     CDS             complement(17304..18002)
                     /locus_tag="KQD82_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG4379003.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            complement(18116..19621)
                     /locus_tag="KQD82_000500"
     CDS             complement(18116..19621)
                     /locus_tag="KQD82_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG4379004.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            complement(19807..21078)
                     /gene="serS"
                     /locus_tag="KQD82_000501"
     CDS             complement(19807..21078)
                     /gene="serS"
                     /locus_tag="KQD82_000501"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG4379005.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            complement(21894..23498)
                     /locus_tag="KQD82_000502"
     CDS             complement(21894..23498)
                     /locus_tag="KQD82_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4379006.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            23884..24810
                     /locus_tag="KQD82_000503"
     CDS             23884..24810
                     /locus_tag="KQD82_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG4379007.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(24930..25928)
                     /locus_tag="KQD82_000504"
     CDS             complement(24930..25928)
                     /locus_tag="KQD82_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG4379008.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            26032..26469
                     /locus_tag="KQD82_000505"
     CDS             26032..26469
                     /locus_tag="KQD82_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4379009.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            26555..27142
                     /locus_tag="KQD82_000506"
     CDS             26555..27142
                     /locus_tag="KQD82_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG4379010.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(27442..28062)
                     /locus_tag="KQD82_000507"
     CDS             complement(27442..28062)
                     /locus_tag="KQD82_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG4379011.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(28070..28837)
                     /locus_tag="KQD82_000508"
     CDS             complement(28070..28837)
                     /locus_tag="KQD82_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG4379012.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVVNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(28878..29699)
                     /locus_tag="KQD82_000509"
     CDS             complement(28878..29699)
                     /locus_tag="KQD82_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379013.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(30058..31968)
                     /gene="phnW"
                     /locus_tag="KQD82_000510"
     CDS             complement(30058..31968)
                     /gene="phnW"
                     /locus_tag="KQD82_000510"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG4379014.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDIEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(32247..33032)
                     /locus_tag="KQD82_000511"
     CDS             complement(32247..33032)
                     /locus_tag="KQD82_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG4379015.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(33249..33479)
                     /gene="dltC"
                     /locus_tag="KQD82_000512"
     CDS             complement(33249..33479)
                     /gene="dltC"
                     /locus_tag="KQD82_000512"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG4379016.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(33498..34646)
                     /gene="dltB"
                     /locus_tag="KQD82_000513"
     CDS             complement(33498..34646)
                     /gene="dltB"
                     /locus_tag="KQD82_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG4379017.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPSVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEIIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(34646..36160)
                     /locus_tag="KQD82_000514"
     CDS             complement(34646..36160)
                     /locus_tag="KQD82_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG4379018.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGRIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(36281..37471)
                     /gene="dltD"
                     /locus_tag="KQD82_000515"
     CDS             complement(36281..37471)
                     /gene="dltD"
                     /locus_tag="KQD82_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG4379019.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(37759..38778)
                     /locus_tag="KQD82_000516"
     CDS             complement(37759..38778)
                     /locus_tag="KQD82_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG4379020.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(38954..39493)
                     /locus_tag="KQD82_000517"
     CDS             complement(38954..39493)
                     /locus_tag="KQD82_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG4379021.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(39594..40274)
                     /locus_tag="KQD82_000518"
     CDS             complement(39594..40274)
                     /locus_tag="KQD82_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG4379022.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(40303..41592)
                     /locus_tag="KQD82_000519"
     CDS             complement(40303..41592)
                     /locus_tag="KQD82_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG4379023.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMIAEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(42040..43641)
                     /locus_tag="KQD82_000520"
     CDS             complement(42040..43641)
                     /locus_tag="KQD82_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG4379024.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDDILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(43752..44429)
                     /locus_tag="KQD82_000521"
     CDS             complement(43752..44429)
                     /locus_tag="KQD82_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379025.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(44442..45137)
                     /locus_tag="KQD82_000522"
     CDS             complement(44442..45137)
                     /locus_tag="KQD82_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG4379026.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(45189..46388)
                     /locus_tag="KQD82_000523"
     CDS             complement(45189..46388)
                     /locus_tag="KQD82_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG4379027.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVEGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(46649..48694)
                     /locus_tag="KQD82_000524"
     CDS             complement(46649..48694)
                     /locus_tag="KQD82_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4379028.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFYNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQELEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(49104..49913)
                     /locus_tag="KQD82_000525"
     CDS             complement(49104..49913)
                     /locus_tag="KQD82_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4379029.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(49997..50530)
                     /locus_tag="KQD82_000526"
     CDS             complement(49997..50530)
                     /locus_tag="KQD82_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG4379030.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(50680..52029)
                     /locus_tag="KQD82_000527"
     CDS             complement(50680..52029)
                     /locus_tag="KQD82_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG4379031.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGADLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(52041..53423)
                     /locus_tag="KQD82_000528"
     CDS             complement(52041..53423)
                     /locus_tag="KQD82_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG4379032.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFVVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
BASE COUNT        17276 a         9400 c         5744 g        21120 t
ORIGIN      
        1 aagtttatat tttcaaacaa gtaatttttc gaaaattgtc taaattcagt aaaaatatgg
       61 taatatatac ataagcaata taaatctata aaaaggagtg ttacatatga tatctaataa
      121 aatgcaaact ttagttgcaa atagttctgt tataagagct atgtttgaag aaggaaagaa
      181 actttctgat atttatggtg aggaaaatgt ttttgatttt agtataggaa atccaagtgt
      241 tgaacctcca gagactatta aagcagttat aaatgatatt ttaaatgaag aatctccaaa
      301 tttagttcat ggatatatga acaactcagg atatgaggat gtaagagatg ctattgctga
      361 acacataaat aaaaaagatg gattaaatct tacaagagaa aatttaatta tgacttgtgg
      421 tgctgctggt ggtctaaata ttattttaaa aactctttta aatcctggtg atgaagttat
      481 tgcttttgca ccgtattttg gtgagtataa aaattataca gaaaactatg atggaaaatt
      541 aatagaagtt cctacaaata tagagacttt tgagccagat ttagatgctc taaaaaatgc
      601 tattactcca aaaacaagag ctttaataat aaacactcca aataatccta ctggtgtcat
      661 atattctgaa gaattattaa aaaatcttgg agaattattg gattcaaaac aaaaagaatt
      721 taatacaagc atatacctaa tttctgatga gccatataga gaaataattt atgatggtgc
      781 caaagttcct tgtgtactaa aatactatag aaattcattt ataggatact cttatagtaa
      841 atcattatct ctacctggtg aacgtatagg atatattgtt gctaatgggc aaatggatga
      901 ttttgatgat gttatgtctt cattaaatgt tgcaaataga atacttggat ttgtaaatgc
      961 tccttctctt tttcaaaggg ttattgcaag aagtttagat gcagaagttg atgtaaatat
     1021 ttataaaaag aattttgacc ttctatataa tagtttaatc gatatgggat attcttgtgt
     1081 taaacctaat ggtacattct atttgttccc aaaagctcca attgaagatg ataaaaaatt
     1141 ctgcaatgat gcaaaacaat ttaatttatt attagtacca ggttcttctt ttggttgtcc
     1201 aggtcatttt agagtatcat attgtgtatc ttatgataaa gttaaaagtt ctttaccagc
     1261 atttgaaaaa ttagccaaat tatataattt aaaataacta aatcaaagta attcaaataa
     1321 ttaactacct tagtatagaa tacttacatt ctatattaag gtagtttata attttatgta
     1381 ctatatttta tacataccat attattccaa tatgcttgaa tattcttatc tatagtacta
     1441 atataaatta caaggaggtg tttgacttga aaaaaggtta tgtttatata atactgacta
     1501 ccttattttt tagttcaatg gaaattagtc taaaaactgt cactaatgac tttaatccct
     1561 tacaaataac tttatcaaga ttttttgttg gcggtttagt cttactacct tttgctgtaa
     1621 aaagattaag agcccttagt ctttctatta caaaatcaga cttaaaatac tttgcatttt
     1681 taggttttat gtgtgtagta gtaagtatgt cattgtatca acttgctgtt ctaaacacaa
     1741 aggcatctgt agttgctgtt ttgttcagtt gtaatccagt ctttgtaatg cttttagctt
     1801 acttaatttt gaaggaaaaa atatataggc acaatgtaat ttcacttata ttagaagttc
     1861 ttggaattat agttataata aatccattgc atacaaaact aactcttagt ggaatattct
     1921 taacattatc ttctgctatt attttttcaa tgtatactgt atttggtaaa agaaaaacct
     1981 taaaatttgg tggaatagtt gtaacctgct ttagctttat atttggtagt ttggaaatgt
     2041 tgatattggt tttactcact aaaattaatt ttatcgcaga tatattaaat aaaaataagc
     2101 ttcatgtatt tgcaaatatc cctattttta gtggatatac cttacacaat atgcccatta
     2161 tgatttatgt attcgtattt gttacaggtg ttggttatgc gctttacttt atggcaatgg
     2221 aagctacatc tactagtctt acatctttag tattcttctt taaaccagta atatctccaa
     2281 tactagcatt attaatactt aaagaaatta tccctataaa catggttgta ggaattttat
     2341 tgatagtaat tggttcaata atttctatta tacctacgat aattactcaa atgcataata
     2401 aacacgttga agccttgtat aaggagtagt aattgataat ctatcatagt tatacataag
     2461 acaaaattta ttagatttaa acgcaatata aaagagatgc cattataata gcatctcttt
     2521 atttatattt attttaaatt tattaaaact ctatttagct aaattttgca aaaagctata
     2581 agtttgtgga caagctgatt ttaacttact atttgtatct tgatttaaat agtaatatgc
     2641 aaatgattct gcaaaaaatt cttctggata tacacctaag tagtttacat tacctaaaga
     2701 acgaccttct ttagcaaata tttgtttgaa ttgtgctgat ttagatatat catttaatac
     2761 tatatggtct attgcatgtg ctgtttcatg taattctaaa tttattgaat catgcccttt
     2821 acctttgtta ctaaatccta ttctaagagc tactgtactt ccacctaaac ctggaacatc
     2881 atcccaagtt tttccagtac cttcccatcc cttaggaact acacctttta aataagcata
     2941 ttctttttca tctgttaact ttccacttaa taattttatt tttaagttat tttgctttaa
     3001 tgcatttaaa tactttccat ctatattagc taatctattt accatagcat tagcttcgtt
     3061 tttatcgtaa tttccagttg gaaaaactac tatttgacta agtgtgtctt tattttgttg
     3121 tatagtagta ctatctgcat gtgcacttac tggaactgaa cttattaaga atattgatat
     3181 tatagctatt aataattttt tacttggtct catattattc cctcctttga gttaggccgg
     3241 gagtaataaa tttcaaaata aaaaagatac ccaagagtat ctttgtatat acaaaaagaa
     3301 atttttactc ttttcggcaa ctggctggca tggtttacta aactttagcc cctatagctt
     3361 tgcgtgacta ggtttcccta gttttgcctc atttaatttt atatctatat tatagcttat
     3421 atctttttaa ttgtctatat ccctttggaa cttttttcta aaaagttatt atttttttac
     3481 ataaatcttc aaaaaaaaat atcttctaac aatattttca ttattaaata ttgttttaga
     3541 agatattttt taatataact tatatttttt ataatgttag ggtctaattt gtatttttat
     3601 ttcttcttaa tacgataagt cctacaatag ctcctataac caaaagtatt tctccaataa
     3661 ttgtagtact atcccctgtt tttggtggta ctggtggatt taccaaagta tcatctttaa
     3721 ctttattatt tttttcttct gacactgatg gctttactac ttctggtatt ggaataatct
     3781 gtttaacagg agtttctatc atctcttctg atggtggtac tggcggattc aatacctctg
     3841 gtattggcgg atttattata tcttctgatg gtggtactgg tggatttata actatagtat
     3901 cctctgtatc agatgaactt gatggtttcc ttggtttatc tgtgcttggt ggtactggtg
     3961 gatttaccat tggttcatct gtatctggag gtattggtgg atttactggt ggattttctt
     4021 gttttagatt ttctatattt ccaatatcta ctatcttacc aatatcttca ttaccaattt
     4081 caaccttaac tttatccttc agtttttcat atccacgtgg agccattatc tccactaaat
     4141 aatatgttcc tgggtttttt acactagatt gaattatacc atcttcatca ctaactaaat
     4201 ccttaactac tatatttcca aatgcatctt ctaaattgaa cttagcacct gaaagtacat
     4261 ttttatctaa agcatccact tttttagctt caaaagcata accttgctta gtagtctcta
     4321 ctcctggagg acttgaacca ttaatctgag ccacatttcg tatagtctcc ccaactttta
     4381 catttgtgaa gtttgcatca aatgtaaccc aactttgcac aggttcccct tcttttatct
     4441 gtttaaattc gtatttattt actaatctta ttgtattttt tccatctcca gcttcttttt
     4501 taagttcaag attatcagaa tattttatat tagatatttc tagaactgag tttacttcat
     4561 ctaaaagatt tacttgtcct gcatcataaa ctccataagt tgacatattg attttgtatt
     4621 taacaatctg gttatcacta ctgtttaaaa cttttttatc aacttctttt tttgcgctta
     4681 ttaatccaga atttttctta aagtttatct catcattttt atcaatttca ctattatttg
     4741 catcaagaac agctctattt atatagctat cttcaatact aattaccttt aatgtataag
     4801 ctataaaaaa ctttgattcc acttcttctt tactgagatt aattgtaaat ccagttataa
     4861 ctttatctac ttgcccactt tcatttcttt tttcttctac tttatatgaa atattattgg
     4921 caattttttc tttactccaa ggagtccagc tcccattcat tggaatttct acaactctaa
     4981 aatcatgccc agcttctcct gaagtagtta atctcatgcc ttctggtata ttatctgtta
     5041 taacaacatt acttattggt gatttatcat catttattga aatctgatag tttattactt
     5101 gaccaacttg agtaatattt gttgctcctg aatgtccttt agtaagaaga taatcatctc
     5161 ctctatcaaa tatacttaca gtagcatcat cttcttgtat cttcttacca tcagattcaa
     5221 tcctagctac attcttatat ttattaccat gttttgcatt tttgtcactg attctagcct
     5281 tatacttaac agtatacgat ttatctatat ttccaaaatt aactgaaata gaatttttac
     5341 tctgttcaat attaaaatca ctaacttctt ttccatcact tgttactaca cttacagaac
     5401 catttatcaa tgtcaatcct tcaggaagct catcatacaa aataatatct tttaactctt
     5461 cctgcttttc atttacttgt atactgtaat ttatatcttt atttttaact ggcataaata
     5521 cagtagttga tttttttcct tcaacaagct ttttaagtgt tcctttagca tctggaacat
     5581 ccgttttatc tccaggtttt atttctacat ctatatctcc acttccactt tctatttttc
     5641 cagtagtttc aatatgtatt tttgcattag tatcttcatc aaccttttta acatatgctt
     5701 ccaaacctat tttaccttgc attacacttt ggccattagg taattctcta aatgtaagtg
     5761 tatactctcc attactttct gttttatcaa agcattcact tgtataatct aaattaactt
     5821 tattgaatac atctggcacc ttaaaagtta ttgaatctcc tgcttgtatt ccttttgatg
     5881 ttggagtata agttaaatta aatgatattt ttttcattga ttctgtttct attgtattgt
     5941 tattaatttt tacaacaaat ccatagtctt cattttcttc attaagttct tcttctaaaa
     6001 ctttcaataa attttcatct tctaatagat tatcttcctc tggagttttt tctgtatttt
     6061 tattatctag tggtttttca tcttctaaag cttgttctga ctttttatta tctgttgact
     6121 tatcttcttc tggtatctgc tctggctttt tattatctat tggcttttct ccttctggag
     6181 tttgctctgg ctttttatta tctgttgact tatcttcttc tggggtctgt tctggttttt
     6241 tattatctgt tggtttttct tcctctgggg tctgttctgg ttttttatta tctgttgcct
     6301 tatctttctc tggagtctgt tctggctttt tattgtcgct ttccttctcc tctccagttg
     6361 atgaattagt tgaaccactt tgaatctgac tattctctcc taattccgaa gcataagata
     6421 cagttggata tattgtagac accaccatac ttaaaatcat aattaaacta attaataatg
     6481 cctttctatt tcctttcttc atgtaaatct caccccttta ggttacctat aatttataat
     6541 ctaatcttcc attaatatcg tatataaagt aatttttact aaaaaattat acaaaaaaag
     6601 ctatgaaaat aaataaattt atattcatag ccggttgcac cactgactca gtatatccat
     6661 aaggtgccca ataatagaat atacttcaac atctctataa tatttcataa aaacaattaa
     6721 aattgcttta tttatattaa aaatacaatt atatacttct attatattat gcctctatac
     6781 tattgtctat cctttttaca ttttttacct tatgatgaga ttaattaata attaaataaa
     6841 atccttgtct acaacaatta cacaattata ttcaggattt atctccttaa ctaattttgt
     6901 tatatcttct tttaagtcat cttctgacat gtttttggaa aattcggaag gattaactac
     6961 tatatcaaat attaaattct tcttgcttcc ttttccaact attctaaaat catgcataga
     7021 ttttacaaga ctatttttct taagtatttc ttcaagttct tttttggctg acataacttc
     7081 tttatcttca agacatattg ggtccatgtg aattactaga tatatatcta atttttcaga
     7141 tatttctctc tctgcagtat ctataatttc atgtatttcc attatatcta tgttggatgg
     7201 aatttctgca tgtatagatg caatacatct accaactcca tagttatgaa taattaaatc
     7261 atgtgtacct attatatatt tataagataa caatagctct ataatagaat ttaccatttc
     7321 ttcatccggt gcttccccta aaagaggatt tatagtatct tttacaagag agaaaccagc
     7381 ataaagtatc actaatgata ctacgatacc aacatatcca tctatgggaa aattagtaaa
     7441 tctagctaca ataaatgata taactacaca acttgaagta aatacatctc ctaaagcatc
     7501 aagtgaaact gcttttaagg ctgaagaatc aattttattt cccataaatt tattaaaacg
     7561 actcaaccag attttaatca ttatagatgc aattaaaagt ataagtggta ttacttcaaa
     7621 ttttacagga attggattta ctatcctttc tatagatgac tttacaaatt gaagtccaac
     7681 caacatcacc ataaatgcta ctattaatgc tgataaatac tctattcttc catgaccaaa
     7741 agggtgttct ttatcagctg gcttacttgc taatttaaaa cctatcatag ttattgctga
     7801 agatgccata tcagaaagat tattaaatgc atcagccatt atagctatac tagatgtgag
     7861 cattcctatg aataccttta tcacaaataa aagtaaattg gataatatac ctactacacc
     7921 agctacataa ccatacttat ttctaacatc tgtattttga acgttttcgc tatctctaat
     7981 aaatgttttg actaggattt tggaaaacat gaaacttacc tccctgccct ttattttatt
     8041 aacaaatttt ataatctatc tctttattta tgataacata atttatatat aaaattctta
     8101 aaaatcactc tacttcagta aaatattatt ctatatatct atttagatac gaattttata
     8161 tttagtaaag tttttatggc ttagtgtctg tttaatagat tctatataag tttttacaaa
     8221 gattatttat tatgataata tcatttttcc caagtattac ttagaatatg atttttttgt
     8281 tttgtctgta catattttaa atttatacta tatttatcta tattttataa ccttatgata
     8341 cactataact ataagatttt aaatggaaag gaatgaaata aatcatgttt tttatcagtg
     8401 atatttacac aaaatcacct attaaatttg atactccttt gcaaaaaaag gcatacaaaa
     8461 tattacaaaa attagatatt gactttgaat gtgttgacac agatgaagca attacaatgg
     8521 aagattgtgt tcagattaat aaaaaattaa atatgaaaat ggtaatataa aacaacatta
     8581 aacaaacaaa aaccgcaaag ttacaatata tagtacttta cggtttcata agaattaatt
     8641 tttactatta aagttcatag ttctactatt tttcattgtt ctaagaatta gctttacaaa
     8701 ttcatttgac aataggatag tggatgcaaa tagtattatc tttatccaca tatcaaagtc
     8761 caaagaaaca gaattaaaga aactctggaa cacttgtgta aatattattt gaattatccc
     8821 tgtaactact attatttgca atgctagttt atttttaaag aaatttggta ttgtactatt
     8881 taaaccaaac tctctacaat ttaatgcatt aaaaagggca ctgaatgcaa ataaagaaaa
     8941 tataacagta ccttgttctt ctgatgtagc tcccaatata ttaaaagctg attgagtaaa
     9001 tacaattgta attataagaa tagcatttat aataatattt acaaacatag acctagctat
     9061 tattccagaa tgtctattta ttggctttct tttcaataca taatctctaa caggttctaa
     9121 tcctaatgct agggctggag gaccatccat tattatattt acccataata attgtattgt
     9181 agtaaatggc atatccttac cagtaagttg agatattata gcaattataa atgctactat
     9241 atttacagta agctgaaact gtataaatct ttgaaagttc tcatatatac ctcttcccca
     9301 ttttattccc tctactatag tactaaagct atcatcagtc aatattatat ctgctgcatt
     9361 tttagatact tctgttcctg aaatacccat tgcaattcca acatctgctt gactaagtgc
     9421 tggggcatca tttataccat cacctgttac agctacaact tcgccacttt tttgaagtgc
     9481 tgaaactatt ctcatttttg tatctggttt acttcttgca acaatagata ttcctttgat
     9541 ttcttctctc aattctttat ctgtcaatac atctatatat gttgcttcaa ctgccttttt
     9601 cccatcattt aatagtccta attcatttcc aatagctgtt gctgtattta tattatctcc
     9661 tgtaagcatt tttacatcta ctcctgcatt aaatgcttta tcaatggaat ctttgacccc
     9721 ttctctcaat gggtccacta ttcctacaaa tccactaaaa actaaattat cttccttcat
     9781 ataacttctt gaatttccta ttaaattcaa ttcaccttct gaagtcattg caacttctgt
     9841 tttagaatta ctcatttttt tataagcaaa tcctaatgtt ctcatagatt taatttgtaa
     9901 cttcttaatc tcatctaaaa tacttttttc tacttttgga gttattggaa caatattttt
     9961 tccttgttgt acataagaac atttttttaa taatacttct ggagcacctt ttgaaagtaa
    10021 tatatctgaa tcctcttgac gaattaatgt tgacatcttc ttttcttctg aactaaatgg
    10081 tatttgtgat atcaaatacg tttgttttct catttcatta tagttcttat cattatgata
    10141 taaaagtaat gcacactctg ttgcgcttcc tatatattta aatgaattat cttctttttc
    10201 tatatctgca gttgaattta ctatacagtt ttgttcaaaa tagctattac tttgatactc
    10261 tcctccactt atatatttac catcaacata tgctacctct accatcattt tgttttgtgt
    10321 taaagtccct gttttgtcgg aacatattac agaaacagaa cctatagttt cacaggcttc
    10381 tttctttgtt actaatgcat ttatctttgc catcttttgc attgttatag ctaaggtaat
    10441 atttatcata gttggcaaac cttctggaac agttgcaact attaatgcaa tacaaaccat
    10501 atatgcattt ttagcaggtt ctaatgattg taagaaagat aatacactgc ttgtatctaa
    10561 aattaacata ccttttctct gcatctgtat aatcataaac atacacaata aagtagctat
    10621 tatgcctgat attcctgcta ttttagcacc caacttccct aatttaattt gaagtggagt
    10681 ttgctggtct tcatttgata aattctgtgc tatcttaccc atctctgtct tatcaccagt
    10741 atgagtgaca accattatgc ccctaccata tgctacaaga gtccctccaa ataccatatt
    10801 gacttgcttt gcaggtattg catcctgttc gattatttct gttttagaat aaactacttc
    10861 catatctaat acagcatcag cattcttagc tacatcatca gattctcctg ttagcatatc
    10921 ttctctaagt ttcaaattta tagactgtat aagtcttcca tctgctggta tcatatcccc
    10981 agtttctata tatacaatat ctcctggtac taaatcattt tttgatattt taattatctt
    11041 cccatttcta agcaccttta cttctatatt ttctgttaat tttgacaggg catgagctgc
    11101 ttttttagat tttccttcag ttattatgcc tatagaaatt ccaataaaaa tagcccctaa
    11161 aatacctaat gcgtcatgta attctcccac aaaagaactt attactgctg ctcctatgag
    11221 aattaatatc ataggttctg tcaaactttc tcccaactcg tcccaaaatg ttctaccttc
    11281 ttttatagta aattcattta atccatatct aagcttcctt tcttctacct cattatcatc
    11341 aagtccaatt tctggatttg tttttaagta ttttaatact tctttggtag gtttattata
    11401 atacctcata actttatccc ccttgttaat tatctataag gaattatatg cttatgatag
    11461 cttgtatatt acttttcaaa aaaaataagg tgttgattaa tcaacacctt atagtggtta
    11521 tatattatta aattttatgt tacaggcttt ataaattgtg agctatacaa atcaaaatat
    11581 ttaatcttca aaataccctt ctggatataa tggaaactta gatataagtt cttgtgcctc
    11641 tgctttacat tcatctaata cttctttatt atcaatattt tctgctacct tattcattat
    11701 ccctgctatc tgtataactt ctttttcttt cagacctctt tgagatattg atgtaagacc
    11761 tatacgaaca ccactagtta caaatggtga ttgtgggtcg tcaggtataa catttttatt
    11821 aactgttatt cctacatatt caagagcttt ttcaaattgt tttcctgtaa gattcttact
    11881 tcttaagtca accattacta tatgattatc agtacctcca cttacaatac taaaaccatg
    11941 tgaaatcagt tcagaagcta gagtctgagc atttactaca acttgctcca ttatcttttt
    12001 aaatttatct gtacttaaat ataatagtga aaaggctttt gctgcaactg tattaagatg
    12061 tatagaacct aatgttcctg gaaaaacacc tttatcgagt ttctttgcat gttctgcttt
    12121 acaaagtaca ataccacttc ttggtccaca gaaagtcttt gtcgtagatg aagaaacaaa
    12181 atctgcataa ggtacaggac taggtattac tttcgctgcg actaatcctg caacatgtgc
    12241 catatctacc ataaagtaag ctcctacttc ttttgcaacc tttgatattc tttcataatc
    12301 tattaatctt ggatatgaac ttgcccctgc aattataagt tttggtctac attcttttgc
    12361 ctttgcttca agagcatcat aatctatacg tccagtattt gggtctaccc cataaaattc
    12421 atatttataa atgtcatgta aaaaattaac tgcgctacca tgtgtcaagt gtccaccttg
    12481 gtcaagacgc atacttaaaa cagtgtcatt tggttttaat attgatgaat atacacaata
    12541 gttagctgta gaacctgaat acggttgtac gtttacatgt tctgctccaa atacttcttt
    12601 tgcacgtttt attgctaggg tttcaagttt atctgcttct tcagaacctg cttgaaaacg
    12661 agcccctgga tagccttctt ctgttttatt tgtaaataca catccactta attcaagtac
    12721 ttctgttggt gctgtacttt cagaagctat catctcaata ttgtgttctt gtctaaccaa
    12781 ctcatcagct acaatcttgt ataactctgg gtctgaaatt tttgttgttt ttaacataat
    12841 aatcctcctc ttaattaaag aatatatttt attagtttaa tcttcattga taaatttatt
    12901 ataacggtct ataaaacgta agatattcct ccaccaggtc ctaatggtat gccaataccc
    12961 atgaatagta acaatattat tatttctatt aatgtaaata ccactgagta tggaatcata
    13021 ttcgatatga cagtacctat acctatatct ttatcatatc ttctggcaaa ggccaatata
    13081 actggaaaat acggaaaaag tggacttatt ggatttgttg aagcatctcc aatacgataa
    13141 gctatttgag ttaatgctgg attaaagtct agtagcataa acatgggaac aaatataggt
    13201 gctaaaatcg cccattttgc agaagcactt cctacaaata aattgataaa tccagatagc
    13261 aatataaatc ctattatcaa tccatagcta ggtatccccg actctgctag taattttgct
    13321 cctgttattg ataaaattgt tcctaagtta ctaagattaa ataaattaat aaactgggat
    13381 gctataaatg ctaggactat atatccaccc atatcactca tactactacc catcatagat
    13441 actaaatctt tgtcactttt tatcttctta cttacttttc catacactaa tcctggagtt
    13501 aaaaatataa tagtgattat aggaactata cctttcatta aaggtgcatt actagatagt
    13561 atagaaccag tctctggatc tttcataaat gcattgtcac ctatacataa ggcaactatc
    13621 actactacta caactaacaa tgataataaa gcgtatttta tacccttatt ttcaagttca
    13681 ctcaattcct gtatatcctt atcatttgtt gaatcattat attttgtaaa acgaggagct
    13741 ataaattttt cagttacaaa tgctcctgca aaagttaaaa ctatcgctga tataaaagca
    13801 aaataaagat tcattgctgg attgccttgg taagatgggt ccatcatttg agctgctggt
    13861 attgtaaatc cagctaacaa tacatctgta gtactaagca taacatttgc tgcaaatccc
    13921 cctgcaacac ctgcaaaagc tgcaaacata cctacaagag gatgtcttcc aattcctaaa
    13981 aacacaagtg ctgcaagtgg tggtaatatt ataaatccta catctgctgc tgtatgtgac
    14041 aacatacctg caaatacgat tgctacagtt acaagttttt gtggaacttt acttataccc
    14101 tttttcatta acacttccat aaatccagat ttctcagcaa cacctgctcc aagcattgta
    14161 accagtacta atcctaaagg agcaaatgac tgaaagtttg taactatgct acctaaatat
    14221 gattgaatct gctctttctc tagtagattt actaccttta caacttcttt tgtagaagga
    14281 tgtatttctt ctactcctaa attagatata actgctgaca atattacaac aattatacat
    14341 agtaataaaa atatggttac tgggtctgga agtttatttc ctgctctctc tacaacattt
    14401 agtgccctca tcaataatcc actatctttg tttttttcct gtgaattaat tttcctcaaa
    14461 ctaatacccc ctcataaact ttatctccag aatgctctat cttaaatttt attttttaat
    14521 ttatccatat aatttataac agactgacat agtacatttg tacctaccaa tatagaattt
    14581 ttatcagctt taaatttagg attatgccac tgatatatac aatcttgttc tttgttgcct
    14641 acccctaacc agtaaaaaaa tcctggggct ttttccatga atatagaaaa atcttctcct
    14701 cctgtagaag gtattggatc aactatagca tcttctgaat acaaatcaca cacagactta
    14761 caagctattg cgtacatctc tttactattt ataacagctg gtaaatcaaa taaataatct
    14821 agctttgcag ttgcttgata tccacgagct atctcacaaa ctatattttc taaattttct
    14881 gatattttct ttcgtacttc attactaaag cttctacatg tgcctgtcat atctaccttt
    14941 tctgaaatta cattatttgc agtccctcca tttatagtac ctattgatat aacagctgat
    15001 tctaatggac ttatgtttct gcttactatg gtttgaatac ccattataat tgcacttgat
    15061 gctactatag ggtctattgt tctgtttggt atacctccgt gacctccaaa tccatttact
    15121 tctatcttaa tagtgtctac tgctgccatt aacccaccta acttaacacc tatttttcca
    15181 caaggtatat ctggatgatt atgaagtcca aatatagcat caacttttgg attttccatt
    15241 actcctcgtt ccactaacat cttcgcacct ttattgactt cttcggctgg ttgaaatata
    15301 aactttacat ttccatgaag actatctcta atatgagaca atacatatgc acatcctaca
    15361 aggcaagacg tgtgtatatc atgcccacat gcatgcatta taccatcata tctggattta
    15421 tacttaatat caacctcttc atatattggc aacgcatcaa tatctcctct tagtgctact
    15481 gtgggaccat catacttccc ttttaataat ccaacaactc ctgtttccat tcctaaatct
    15541 aatatttcta tgtctaaact cactaataaa tcttttatag cttttgtagt cctaaattct
    15601 ttaaaactta attcggggtg catatgaata tctgtaaaaa aatctaaaat atcttcttta
    15661 tacttttctg agattaaaat aagttcatgc atactaacac ctctccaaaa ttttattttt
    15721 ttatttttta taatttaact acaaattata taagttaata atctttataa agatattttt
    15781 tataaattta attttagttt atatttatat tttactatat tattttaatt aaatatatta
    15841 tcatgttttg caatttattt aactattttg actttttaaa ttcggattat ataaaaaatt
    15901 tttttaaata aaaactatta ataagtggct ctattcctga ctttgtgttg atttttttct
    15961 acatgtaatt gctttataca ccatacagac taaaattaaa agccccatat aacctgtata
    16021 tggatataaa atgtttacta atttaccaaa cggtaaaaaa cctcctatac aagccaaaat
    16081 agctattact gttgttacca atttaaattt aggatgttca tcttcaacaa ttctattggt
    16141 tacagaccat aaaagtggaa cagctgttgt atagatacct aatattaatg caactgaaaa
    16201 acatacacct acaatcggag atatattatc tgctaaataa agtgatggta tttctttcat
    16261 atacaaattt cctatatctg aaatcatagc caaatacata actataccag cagtcataaa
    16321 cactattcca cctaataaac ctccccaaaa agcatccttt ttactttcaa tagaagctcc
    16381 cattcctgtt aaaaaaacaa tcattacaat aatatttaat gtattatata ttacaccaga
    16441 aacgattgga ctttctgatg cttttgtaac ttcaatattt ttcatagctt ctccaaccat
    16501 agataaaccg tcaaaatgct taaacaagct tattaatcca acagcaattg taaatactgc
    16561 tatcactggt ccaatacgtc caagtatgct tactaatttt tctaaaccca tagacactgt
    16621 aattaaagta gcaacagcca tcaatactct tcctaagtaa ggatttatac catagtattc
    16681 agttaaagtt gagcctgcac ctgaaatcat tactataaga atcccaaata aaaatattgg
    16741 actaaaccat tcgtaaaatt ttcctaaaat cttcccacaa taaaaagtgt aaattcttgt
    16801 tggttcttta agttttaatt ctctacctct agttataact tcagcaccaa gaaatgaaaa
    16861 caataccatt gatattaatg ctccaattaa acccatatac ccttgtgagg caaaaaattg
    16921 tagaatttct tgaccagtag caaacccaga accaattgct gttgctagat aagcgcctgc
    16981 aatacagata atatttttca tattcacttt gtctgacata ttaaattacc tccattataa
    17041 agttaccttc ctttaagaaa tataaactct cagtaaatta aatgaaaatt ctgtcttttt
    17101 gaaacagtat ttaattcact ttttttattt tgtatatctt tttcttttta tttatatata
    17161 tttttttata taaataggta cagcttttat aaggtttaat attattaaat atgtttaaag
    17221 cgctttaatt tttatcataa ttaaagtata cttattcaga cacactcaaa caattatcta
    17281 tattgacttt ttatttatca atttcaattt ttagtgttca gtttataaac ttttggagtc
    17341 attccttttt ctatcttaaa gtttttataa aagttattta ggctattata tccaactatt
    17401 tctgcaatct catttatact ttttgtagta ttttcaagta actcaataga tttttctatt
    17461 cttatatgat ttagatatga tataaaagtt gtatttaaat tacttttaaa aatcctagat
    17521 atattagatg gattggttaa aaataatcta gcacaatcat ttaactttaa gtcattgcta
    17581 tagtttagat ttatatattc cagtatctca tttaataaat cattattggt atattgatat
    17641 ccaataattt tttctacaga cttagtaaat tctttcatat ccaatggttt aaggagtata
    17701 tcttttacat ttaatctcaa agctttttga gcatattcaa aatagttaaa tgcagttatt
    17761 actacaaagt ttattttttt agataaatga gatgtttttt ctattacttc taacccgttg
    17821 tatattggca tttgaatatc tataaaaact atgtctggct ctaaaccaat gattttatct
    17881 actgcttcat caccagatgt tgctttacca acaattttta ttggcatatc tttcatttct
    17941 ataaaatgtg ttattatttt tagaacagct tcttcatcat ctattattag tgctcttctc
    18001 atgagaatac ctccctttta gcttaataat ataaaaaagt aagatatata aaacaacata
    18061 aaaacatcta atatatttct taacatttat aaaacttatt aatttttcac aagttctata
    18121 catactcctt ataattttca ataggaattt taatcattat gtaagttcct tcattatcac
    18181 tcgactttat atccatatca aaattatttc cataagcaga tgtgaatttt gtatatatta
    18241 aagacaaacc atgaccatta ttactacgaa tacctgcctt tattttgtct atattatttc
    18301 catctaagat agttccatta ttatgaatct ctataatagc atactgagaa tctaatcttg
    18361 caattatttc aattcgcttt tctgtgtcaa tatctctaaa tccatgtgta aatgcattct
    18421 ctacaatagg ttgaataaag ttaaaaggca cagaaaggtt atataacttc tctggaataa
    18481 gatattttac tttaagtgca tctccataac gtaatttctg taaattcaaa taatttttaa
    18541 tgtataatat ttcagattct aattctacaa atcttaaatc agacctcatt gtatatctaa
    18601 acattcttga taaatctatt attgcactat acaaatcata actaccatca tctagtgcta
    18661 tgcttgccat tgaatttaat gtattaaata aaaagtgatg atttattctt aaattagtaa
    18721 ccatatcttg agctactttt aaattcattt caagttgctc tccataatga tatgttcttg
    18781 ctatagtctt ctctttttct tgtaaatcct ttcttatatc attaaaagag caaaaattta
    18841 ttatattttt agatatttgt ttcaggaggc gttttatact tctagcagct ccctctggaa
    18901 tatcatataa attattatgc tctgtatcta aattcaaatc agataatagt acataccctc
    18961 ctcttataac acctatgcta ttgcttttgt acagtattgg tatattatat atagttatcc
    19021 catattcaca tacaaattga ttgctacttt tttgattcat acaatcacaa ttttgaggaa
    19081 atttcatagg attacatttt tcaaaacaaa aaagtggaaa ataaggattt tttaaaacta
    19141 tattccatga atcatcatat ataattaatg gaattttaaa cgggtctata aattgaccac
    19201 gaattgattc tactgctgca tacaaattta atgtactttc actataattt atcaaggtct
    19261 tctttaaata attattagga ttaatatcta taacttcact ctctcctaca tcatctacat
    19321 tatttgaaac aataagttct tttatttctc tatcacctgt atttatagtt tcatgtgtgc
    19381 taccagattc tatataaaaa atcatacctt gataaaatgg cttccaaact ccatttataa
    19441 tatacaaacc ctctccctct aaaatatata tcatctgctc ttgaccataa tgaacatgat
    19501 taaaatgttt cttaccaggc atagttactg caatgtatat attcatagct tgttttggat
    19561 tattttcatc ataagtatgt ttccattcaa tatatcccca ctcagtattt tgaattttca
    19621 taaaagattc ctttcctata aattatttat gttcatattg tgaaaaatta gtaaaaaaaa
    19681 tgtgtacata ctctttcata tgatagctta ctatatcaat attcattagt ttgtacacat
    19741 ttttatatta attatttttt attacctcta tattattgta ttgcttaaaa atacataatt
    19801 taactcttat ttaataacag aaactcccat atatggtctt aaaacttcag gaacaaccac
    19861 agaaccatct tcttgttgat aattttctaa tatagctgca acacttcttc caatagctaa
    19921 cccagagcca tttaatgtgt gtacatattc agctttagaa tctttatctc ttttaaatct
    19981 tattcctgct cttctagctt ggaaatcttc gcagtttgaa caagatgaaa tctcaacata
    20041 cctattgtaa cttggcatcc aaacttctac atcatattta aaagaagctg taaatcctaa
    20101 atcacctgta catatcttta ctactctata aggcagattc aataattgta gcatagtttc
    20161 cgcattgtta gtaagctttt ctaactcatt gtaagattct tctggtgcaa ctattttaac
    20221 catttctact ttgttaaact gatgctgtct tactaaacct cttgtatctc ttccagcaga
    20281 accagcttct gacctaaaac atggagtata agctgtataa tttatcggta attgttcaaa
    20341 tttaagtatt tcattagcat gtatattagt taaaggaact tctgatgttg gaatcataaa
    20401 atagtccata ccttctattt taaacatatc ttcttcaaac ttaggtaact gtcctgtacc
    20461 taaaaaacta tttctatttg ccataaatgg tggaataact tctgtatatc catttacttt
    20521 tgtattcgtg tctaagtaaa aatttaataa tgctctctct aatctagcac ctaatccttt
    20581 gtaaagagta aatcttgaac cagttatttt actagctgtt tcaaaatcaa gtatacctaa
    20641 ttctgttcca atttcccaat gagccttaga ttcaaaatca aaggtagtag tctttcccca
    20701 agttcttact tcaacattat cttcatcagt ttcaccttgt ggtacttctg gatgtggtac
    20761 atttggtatt ctcattaata ggtattccat cttatcttct acttctttaa gattttggtc
    20821 tattacctta attttatcag ataactcttt taatttaatc ttttcatttt ctacatcttt
    20881 accttctttt ataagatttg gtatattttt agatgctgta tttaattcat ttttcataac
    20941 ttcaacttct tgaagaatct ttcttctttc atcatctaac ttaactactt catcaaggtt
    21001 aaagtctttt tcgccccttc tttccattgc tttttttata caatctaaat tttctcttat
    21061 tctttttata tcaagcattt tttcctcctc agtacctaag attggattta tctcttaatc
    21121 tcaaataact tagataatat acttcatgct tgaggttaag aaaccttttt ctttatccga
    21181 acctgttatt tttattttat ttatatttta ctcacttttt tgcccattgt caaattatat
    21241 ttaattaaat aaatgaaatt taattttagg agaatattgc tagatttaac ttcattataa
    21301 ttatatctat tttgctaatc aattatctat tttgactttt tctttatcaa tcttgtatag
    21361 ttattaactt ttatataaat ttttttatat tataacatag tcctatatat ctattatttg
    21421 gatagaatta ttttttaaat tagaattatt ttgatgaatg attcaagtaa taggtatgaa
    21481 attgaatctt ttagaactgc ttacaaaaaa tactcaaaga aaaatagtaa ttttctcaga
    21541 ttcacaatat attttatatg aaatgttata aggtaatcta tttaataaat ttaaatttgc
    21601 tattatagat ttagttccaa agtcttactg caaagtaata tacatagttt tttaataaac
    21661 taataagtgc aattaataac tacttaattg tttaatatag tacatagata actttaataa
    21721 atctacagtt atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa
    21781 taattaaaat ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc
    21841 taacttatta tataaaacta ttttttatag attataaaat atctaacttt catctacttc
    21901 aaagaataca atacttctcc ttctcttata gtctgcaata ctttaatatc ttttattttc
    21961 atagggtcga ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct
    22021 ttaattgaac cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc
    22081 tttaaagcat ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta
    22141 attctattga cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc
    22201 tgatgaagag tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac
    22261 gctctatctt ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca
    22321 aagtatgaag gtatcatatt tattactttc atatcatcaa tctggtctgc tcttactgtt
    22381 tgagcatgta tcataactgg tcttatatta ttttcactac tttgctcttt taagtttaaa
    22441 actttctcaa acgcatctat caactggtct gctgctgcat ctccattaca atgagttaat
    22501 agttgcatct tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat
    22561 ataggatatc cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc
    22621 ttaccttgtg gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata
    22681 tattttttta taaactccct attattatct actacagact tacttttttt caaatttaca
    22741 tatcctacaa catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat
    22801 tgattttctt ccattaaacc ttcttgtgct gttgtaatac catagcttag atatatgttc
    22861 tgtgcttttt caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct
    22921 acattaaaaa atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta
    22981 cctatatgtc caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc
    23041 aatgtatttg caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa
    23101 gatgcactat cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca
    23161 tatgaaaatc caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt
    23221 atttttttta ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta
    23281 gtagcaaaag ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct
    23341 tgtaaatcta taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct
    23401 atctttttta ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt
    23461 gtgatgatat ttccattgta ataaattttt tctttcataa aattctcctt acgttgtcta
    23521 ctttaataaa tttttttcat tattttctgc ttaattatat tatcttcttt ataaactttt
    23581 tctttagagt ttaatcatat aaaagtttta tttttaatta ttgtttgcca agaagtaaat
    23641 actattcatt gtaaataaga caaattaata ggctttatac taattgaaaa aatattataa
    23701 gttgttaaat ttaaatatct aaactcaatt atatctgtac aattgtaatt ttttaaatca
    23761 tgatgtatta ttttaaatat acagttacat tattattcac aatataagta tcttagttct
    23821 caaataacat ctcatgtaat ctattcaatg caaaaaattt aaatattttt taggaggttt
    23881 tttatgagca aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata
    23941 atggatttag aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc tggtagaaaa
    24001 ggggcaaata tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca
    24061 tttggatttg ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat
    24121 gataattctg taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa
    24181 gcaggagtat atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc
    24241 ctttattgca caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa
    24301 cttaaaccta caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca
    24361 gtagttgata ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca
    24421 ttagctagaa cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat
    24481 tcaagagaag agtggcaagc aactatgaag catattgctt tagaaggaag atgttttgta
    24541 attggatgca atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa ctactataaa
    24601 gagctagatg cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt
    24661 ggaaaatatg tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac
    24721 ctagaaaaaa tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca
    24781 gatgtatttg aattaattgt tcatgaataa tctttttaca caaacatcta cataaatctt
    24841 tttacataaa aaaatgagat actcacaagt aaatttcaag tatctcattt ttgcttaaaa
    24901 cctataatcc taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt
    24961 ccaacatgtt ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact
    25021 acatttacca tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt
    25081 tttaatctta agttcattgg gcaatccaca gaatctatac catataaagt aattcccttt
    25141 aatataaatg gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt
    25201 actgctccac aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta
    25261 tctattactc cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct
    25321 gacctatgta taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat
    25381 tttcctgttg aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca
    25441 gcaactcctc cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct
    25501 ataaatttat acacagataa agctgctgta aaccctgctg taccataatc cattgcttct
    25561 tttagtgaaa gatttttagg aagtttaact acccaactag aaggaactct tatgtattct
    25621 gaaaaaccac cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca
    25681 attttaaaat catcacaact actagattct actataccag atgcatcaat tcctggtgta
    25741 tgaggatact ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt
    25801 aaagaagaat attttacttt tattagaact tcgccttctg gcaatgtatc tacttctctt
    25861 tcaactatat ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt
    25921 ttattcataa atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa
    25981 atagttttat gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat
    26041 aaatatattg tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat
    26101 aaactcagta aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca
    26161 ctttatgaaa ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat
    26221 aaatccactg ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa
    26281 aaatgtacaa atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata
    26341 gaagatactt ttaatagcat ctcaacacaa gttaaagaga cagcatacca taatattaca
    26401 gaaaatgaaa aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat
    26461 gctgactaaa ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat
    26521 attgtaaaaa tttattatga tagagaggta gaatatgaat ttaaaagata gtaaaactaa
    26581 agaaaattta ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc
    26641 tgcatctgta gcaaaaaaag aaggattata tataatagag tctttattta attacactgc
    26701 tgaccaagaa aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga
    26761 aaatataagt atatcaggag cataccctgt agatttatat gacaagacag cacaacacct
    26821 taaagctgca caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa
    26881 aacagcacgt gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat
    26941 agaaaaagtt catggtgata gatttggtag atatgctact gatttagaag aaggtacttt
    27001 attcagaaga aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg
    27061 taaagaagct ccaaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt
    27121 taaaaactca ttatttgaat aaattaaggc aataattcta tttaaacaat ttactatatt
    27181 tatatcaagt atttttataa atactgtatt atcttaataa caataaaatg aaccattgat
    27241 attactataa gtagaaaatg tagtttaaat acaatttata gtaattcatc atggttcatt
    27301 ttattattta ctaatcccta taacctactt aaatctatct ttaactttta cacactcaca
    27361 taaaatctta tctttaaact aaatatttaa ataaagtgta ttttaagtat attattataa
    27421 tacattatat aagataatac actatgataa ctttatactt atatcattta tttcttttgt
    27481 caagtcaatc tcaataatct taactacatc tttttcaaac tttacttttt tataatccag
    27541 acatcctact acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt
    27601 gtatattgat gtaaaatttt cttctattat attcactatc aattttaact cttcaaaagt
    27661 tattatagat ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc
    27721 tatgctttgc tcttttttat ataaattttg aaatatttct ttggtataat tacatataac
    27781 ttctttactg agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat
    27841 atttgcaata tcatcaagag aagtctcctc tttcatagac aatattaact ctattctttc
    27901 tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa
    27961 ccattcctct ggtattatgt tttttctttt ccatctatat aattgaccat aagatatatt
    28021 agttttgctc aataaatctt tcttagatat taattcttcc ataacttttc tagcccacct
    28081 ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg tattcaatat
    28141 ttacaacgtc acaattatct actagagata tgcttttgcc tcgtatatct tttattgatg
    28201 catcttctaa ttcaatagta tctccctcta tcaaattaca tttaaacttt cctgaaaaaa
    28261 tattaatgat tttaattaat gaaaaagcat ctttgcttct tcctattttt atattagttg
    28321 cccctatctc tttacaataa gaacctcctc ctgaataaac atatatattt tctccattta
    28381 aaagaccatc acattttatt gctccatata cctttatatc ttcacattct atatttgatt
    28441 tatttctcat ttcgccatag atttttatat cttgtgcata catattttca tttacactta
    28501 attgtcctct tattgtaaca ttattacatt taagtttgcc atccactttc tgttgagcag
    28561 ttataactaa gtcatctatt tctgcatctc cctctatatt aagttgacca ttcactctta
    28621 cacgctctgc tttaagattg cctcttataa tagtttttcc gttaacaaca aaatttttta
    28681 tattgatgtc accttcaaat tttgcatctc caaggacttt tacttcgtta cttttaacat
    28741 ttccttttga tttagagctt cccacgattt ttatattttc atactcacca ctacttataa
    28801 agccttctcc aagtattttt atagaccctt tttccatatt attacctcat tttaaatttt
    28861 atttttaaac ctacttatta cttatccttc tttaatttta ttttttcact tatattcttt
    28921 atttcaccaa ctattgaatc ttcatcaatc tctatacttt cactgtactc caaactttct
    28981 acataacatc ccttgcctat tttaatattt tttcctctta caactttgac ttcactattt
    29041 tctaagctta tttcatctgc ttctataata tttgcaaaca atttactttc tgtatacttt
    29101 ctaattgata atagacctaa acttattaaa tttgaagaca ctttttcaaa gttgatatgc
    29161 tttttttcta gcatacgaaa ttttttgcaa taatctccat gccccttttt tatatttata
    29221 ctacttgctc caacttcatt aaaattagaa gttccattta aactaatatt aacttcttca
    29281 caattcaaaa atccattaca ctccaagatt ccacttaaat tgatattttc acaatctata
    29341 ttctcatttg cttttaaata tccttctcca tgaataatat ccgattttat acttccttct
    29401 acattaataa ttccactagt atcaatttca acagaattaa tatttcctgt acatctaaga
    29461 agtcccgata tctcaacttt tgatgccagt atgtttcctt ctactttact taaaccagat
    29521 gtaaaaaatt cattacattc tgttgtccca aaaacttttg ctgaaccaga agaatcaaat
    29581 ttactacatt ttacacttcc ttctattttg cctgttcctg atatattaac attatcaaaa
    29641 attccacctt tacattttcc aacaccatca aatcttagtg aacttaattc ttcattcata
    29701 atgatatcct ttctttaccg tattttatct ctaaacctaa tataacaaaa cattgttacg
    29761 ttgtcaagta tgataataat acttaataaa gcaactatca tttattttct ataaataggc
    29821 ataaaaataa tagggaaatt ctttatttaa actataaatc taatttctta gattgtggtt
    29881 taaataaaat atatccctat tactcaatct ttatatattc attgtataaa cttctaaaat
    29941 ataaatcttt aaattctact gtcaatactt tttatacact gtttgtgcta tcatctcaca
    30001 cacctaaagg agtgggcttt ccacactatt ctgtaaccta caagacacta tcttcttcta
    30061 tctattgata aatttttcta ttacatcagc tagtctctcc atatcagttg gataaacttc
    30121 acctatgcta cctattctaa atgtatctat atcagtcaat ttacctggat agataacaaa
    30181 tccattatct tttaaataag tataaaattc cataaattca aactttgcat ttttaggata
    30241 taagaaagtt gttattacag gtgattgagc attatcattc accaaagtat caaatcctaa
    30301 agattttaat cttgaagcta ttgtaaactg attttcttta tatctagcat atcttttttc
    30361 aacagaacct tcttcttcca attctaataa agcttgataa aaagctctta caacatgtgt
    30421 aggtgaggta aatctccatt taccattatt tttctccata gtctcccatt gcgcatacac
    30481 atcaagagat aatgatttag ctataccttt acatttagat aatttctctt tattagctat
    30541 tataaaacca aatccaggta ctccttgtat acatttgtta gaactactaa ctaaaaaatc
    30601 tatattaaaa tcttcaacat caatctcaat tcccccaaaa ctactcatag catcaactat
    30661 ataaattttg ttatattctt ttgcaagttt ccctacctct tgtataggat ttaatctacc
    30721 tgtagtagtt tcacagtgaa ccattgatat atgtgttata tccttgtttt cttttaataa
    30781 attttctact acatttaaat ccactgcttc tatatcttta aaagtgcagt caacaaattc
    30841 tatgttaaga taattacata tatctttcat tctctttcca tatgctccat ttgcaattac
    30901 aagaagttta ccatcttttg aaatagtact tcctattata gcttctactg aaaaagtccc
    30961 acttccttgc attaatacac ttgtgtattt atctgtattt tgggttgcta gagatactaa
    31021 tcttcttcta acatcttgta ctaggttatt atattcaata tcccaagtac accaatcttt
    31081 taacatagat gctcttactg atttagtagt agatagtggt cctggtgtaa gtaatatgta
    31141 atcattttct ggtacaaaat ctgatttaat agtttcattc tctattttta ttagtatatc
    31201 ttctaactca gccatagttt ctattacaaa atgtgcacct gcctctttaa atttctttga
    31261 aactatactc atcttagctt ttaattcttc cttatccatg ttctctactt cttcttgtgt
    31321 aagccctaac tcacttgaac cttttattac agcaacactc cacattcctg catttacgcc
    31381 ttcttttaca tcacttattg tatctcctac ttttaccata gaactcatag gagacacccc
    31441 taaagcttca gcatttttat agcacatcca aggatatggt cttccttgag atacttctga
    31501 tggtgttaca agaaaatcgg gtgagtatcc tttctttgct gcatttggtt caactatgtt
    31561 catcatctct cttgtataac ccgttgtaga acctattttt aatccatttt ttctcaactt
    31621 ttcaatagtt tcaacaacat gaggaattgg agtcgtataa tcttctaatg tctcaaataa
    31681 cattggctca aattcagcat aaagttcatt tacatcatct tcagttggca ctttaccaaa
    31741 tttatctgac caaatattct ttattctatc catttcacac atttctctta tatggtctat
    31801 ttttaatttt cccataggtt ttcttgcttc ttccattgtt acatctatac ctcttctttt
    31861 aaatatctct ataaatacat taagaggtgc aaaacatcca taatctactg ttgttccagc
    31921 ccaatcgaat actactgctt ttattttttc tccatatatc tttttcatat ttatagcctc
    31981 catagattct ttttagaatt tttgtgtttt taattgtttt tacttttaaa gtataacttt
    32041 catatgttaa ttatacatca tatttaggtt aaatatatat taaatttctg tttttagttg
    32101 tatttagttg tttttatttt gtatcatatt cccataaaat cctaagttac aatctaaaat
    32161 ttactttcta atcttaatct atgaactgac tatattttat gaatttagtt gtattgagca
    32221 tagttgtatt atataaaact aagggtttat tttgcttcta tccatgttat atttatctca
    32281 tttaacttat ccttccattc attcggaggt tctacatcac aaataacaaa atctaattcc
    32341 tctaaatctg ctatcttata catactagca attcctattt tagaactatc acataataca
    32401 aaactttcct ttgaattttt tatatattct tttgacaaca atgccttatt ttggtctata
    32461 ctactaattc caaaactatc aagtatacct tcactagata taaaagcttt atctacgtat
    32521 aagtttttca tagtatctat tgacattgga catgctgtcc tttggtgttt taaatttact
    32581 tttccaccaa taaatataac ttctccatca aacaaacctt gattctcata agaaatcaat
    32641 tttgatagaa ctggataaga gtttgtaata atttttaaat tgtttacaga tattatataa
    32701 tctacaatct gcaaattggt agtaccttca tctatgataa ttgactcatt gtcatttaca
    32761 aatttagctg catatttagc aatactttgc tttttatcta ggttagtatt gtttctcttt
    32821 atatgttctg gttcaatcac tccataattt atttttatag caccaccata tacctttttt
    32881 aatttatttt cattttctaa ttcttccaaa tatcttctta ttgtttctga tgatacttct
    32941 aatcttttta ctaattcttt agtgtttacc ttcccttttg aatctaataa tttaagtatt
    33001 tctttttttc tatctatcga atttaaagac attcattttc ctcctaaata taatatattt
    33061 ttctcctata atattacaat gatacctaag atttatgaaa tcatatttat gtattaaatt
    33121 ttttacttaa ttaaagttat tttcaaaaga ctttgtatta tcattaaaaa agtccatcaa
    33181 atattagtat cagatggact ttgtaaaatt ttattttata aattaatatt taaagtctaa
    33241 ctactaattt attttcttgt ctccaagaat tttactaaat tatttacagt agacatatct
    33301 tttctctcta aatcagttgg ttgaagctct atccctaatc tattttcaat ttcaagtaaa
    33361 acttcaatta tagctaatga gtctaaaagt tccgtctcaa ataaattaag gtctaaatct
    33421 tctcttatct catcagttcc taatacatct tcaaatattt ctataactgt ttcttgcata
    33481 aattttcctc cttaaaatta agctaaataa cctgagaata tcagcaatcc aaaacacaca
    33541 atatggaatg taatgaaaat ctgcactctc tcgaaccatt tatctttttt atgtttttta
    33601 taaaatttag attttcgctg atatatatca gttaacacaa gtgccaatcc ttggtataca
    33661 ccataagcaa cataatacca agttagacca tgccaaaatc ccattgttat catggttatc
    33721 atctgagcca catgagcagc agtagtcctt ttcttaaatc ttttctttct cattgatgac
    33781 ataacaaatc tagaaaaaat ataatctcca aaccatcttg aaagacttat atgccatctt
    33841 gtccagaact ctttcatatc tttgctgata aatggcttgt caaagtttat aggtacttgt
    33901 attccaaata tgtaccctgt acctatagca aataaactat atcccgcaaa atcaaagaac
    33961 aggtataaac tatatgcgta catataactt aaaatattta taaaactcat atctttaggt
    34021 attcttgata cccaatatgt atttatcaaa aatgctataa caaacttata acctactccc
    34081 atgacaatat tttttattcc tggcagtaag tattcttcta tatactcttt tctagagatt
    34141 tgtttttcca agtcctgttc aaatcttctt gacctatcaa taggtccaga acttaaagtt
    34201 ggaaaaaata acataaaata aagcatcttt gaaattttaa cttcttttat agctccatca
    34261 taaatttcaa taaccatttg tatagttctg aaatttaagt atgatattcc aataaatcca
    34321 attattccaa aagaagtaac tggagatatt ttatttatta ttattgggag cattgatgca
    34381 aacaagaatc cccaatatat gtatttattt ttagtttttc ttcttacata ttcatatcct
    34441 ttaactatta ttatttcaag aataataaat attaccaagt actttagttg gacatcaatt
    34501 cctactatta agaatatcat aaacaaactt gccaacatac cataatactt tatattcttg
    34561 cccattagac ctaaaattac tgatggtatt gatgttaata ataatatata aagataaaaa
    34621 taatctccat attgtgaaaa aatcattaaa tttcctccat aagtttcttt ctgtctattt
    34681 taccattgat atttgttgga aactcagaga ttattgaaat atttcttgga atcatatatg
    34741 aaggtatata ctttccaagt tcctttttta ttatcctccc atttttaata ttacttaaat
    34801 cattcttttc atttaactca acaatacctt ttaagtaagc aattttttcg tccttatata
    34861 ctggtaaaac tactgcattt tttacattat gtacttttct caaattattt tctatatctt
    34921 ctatttcaat tctaaatcca tttaatttaa tctgaaaatc ttttcttcca caataatata
    34981 tatttccatc taaaagatat cccatatctc cagttttata agctctccac tttacaccat
    35041 ctatttcatc atagaaaaat acttcatcag tcttttcttt attattgaag taacctttag
    35101 aaactgatgg tccaattatt ataatttcac ccttttcatt ttctttaagc tcatttccat
    35161 cttcatcaag tatttttatt ttacaatttg acatagggta tccaactgga agactttttt
    35221 catcatctat agccttttga gtcatatcat ttacacttac accaacagta gcctcggttg
    35281 gtccatatcc attgataatt cttgtgtttg gaaatctact cataagttct ttagtaagat
    35341 tttttgaaag tgcttctcct atgaatatca ttgactcaag gttaggaagc attttactat
    35401 taaactcttt ttcagttata catactcctg caaaagaagg agttgacacc catacagcta
    35461 tatctgagat actaaattgt ctaaatagtt ctctatagtc tgctaataca tcttttgata
    35521 ttgaaaataa tgtagctcca tggataagac cagggtatat tgttgtaact gaaaggtcaa
    35581 aagaatatgc tggttggttc attattactt tttcacttcc atctatgttt agatatgggc
    35641 ttatccaatc tgaaaagcta tctaaattgt ttgaacttat ttgaacccct tttggctttc
    35701 ctgtacttcc tgaagtaaat agtatatatg cattttcatc atcttttacc cagttttctt
    35761 tatctaatga ttttccctga tattcattta ttatataatt taatttatcc atatcaatta
    35821 cattgatatc tccaaaattt ctttcatcac taaagttaaa taataccttt ggttttatct
    35881 ctttagtgac ttcaaaaact ctatcaattg gaaaactaat atcaagaggc acatacgctc
    35941 tgcctgattt taaagctcct atcatacatg ccattatcat gttttcttta tttccataaa
    36001 ttactatagg agtatcttct tctttgtata catcctttag aaatactgat atagcatctg
    36061 aatattcatt taaatcttta taagaaagtt tatctccatt acacattagt gctgttctat
    36121 ctgtatttga atattttttt attccttcaa taattttcat ataagaatgc tcctttaaaa
    36181 tccgttataa ataaatggca attctttaaa tggagtaaat gttagaactc ctatcgccaa
    36241 tattataacc atagttaaac aaatactagt taacaaatct ttattgttct ttaaatattt
    36301 taaataatct ctcacagaca tctacccatc ctttcgtacc aagatgcatt acatccctta
    36361 aataatactt atctgaccct ttatctttaa ggtttagaac ttcaaagcct ttgctttctg
    36421 ctattttttg tgctttatcg tagtactgat ttctttcttt ttcagaaata ccagtcaaat
    36481 tatagaattt atccatactt gggattaata caacaactgg cttaatacct aagtcagtac
    36541 atacatttag catcaattta taattttcaa actccttaga tgttaataaa tttacatctt
    36601 tgtctcttcc tttgtattgc tctataccat ctttaaaatg ctctttataa tagtacttat
    36661 caatatttag tggattttta ccaactcttt ttttagcatc ttcaatggct ttctttctct
    36721 catatgacca atttattggt ctttttcttt ttgttgctct ctttttatct agcttaatta
    36781 atcgtttata aagaataccc ttttctttaa gagctataca atactttctt ccttgaaaat
    36841 aaggctctaa aagcaccttt tcagcttttt caggaaatgt cttaggctca tatagcttag
    36901 catataatgc ttctgcttta tactcatcac ttccccataa taacttactt gatttttttg
    36961 catattcaat cttattttgt ttagatattt ttggattatc taaaaatcga taaaattgta
    37021 ttggtgaaaa tctagactga taatggtgac ttgtgactcc gtctttttcc ataaaccatt
    37081 gcatagaaat taacaagaca actttcttat tatcaatatt aggattcata cttcctaata
    37141 ttgcagcatc ttgtaatgtc tgagtatagg ctcttccaat tgcaaacacc ttatttttac
    37201 tcctatttgt attaaaatag tactttggat gttgttttgt tgaattacta agttctgatg
    37261 accccaaaat cataatatcc ttttctctta aaaaatgatt atttgctgta acccctttat
    37321 cttttatatc acttaaagta tcatccataa ttggaagtag atttttctcc ctcaaaagtt
    37381 catcagtttt agaatcaaga aatttgtcta gcccaaataa aaatactacg cctattataa
    37441 aaggtgtaat aaagtatatc aattttctca tattttctct cctctaaaaa tattcaaatt
    37501 tatgttatta ttaatcatat tagttttgat atgtatattc aactacaaaa ttgtaataaa
    37561 atcgtcaaaa ctttttttga tactgttaca aaattgtaaa aaaaaagaaa aaaacgccaa
    37621 aaataaattt ctttttagca ctttttcata cttataattt gacattttaa cacttggtta
    37681 agtatacgcc ttaccttaac ttaaaattaa atttatttaa gaatatatat tataaattct
    37741 tatattttct aatataaact atatactatc agctggataa acaatactac tttgtcttct
    37801 tatctcatcc ataatttcca ttactattat tgaattttca tgagaattta tatttgattc
    37861 tattttattg ttatttataa gacttataaa ctctacaagt tcataataca ttccttctat
    37921 ttctctaggc ttttcctctg ctttttcttt tggaatagat atatcttctt ctcttccatc
    37981 tctgtattta ataatagctt tttcaaatag attaagcttt tcaattatta tacttccctc
    38041 ttctccttgt atttcagatg gtatataaga atcagctact ttagaatact gaattatcgc
    38101 atccatatca tcatattgaa atacagctga accttctcca tccactccag tttgaagtat
    38161 atgagaaact gcctttacag tttttggagc accaaataaa ttcaccatag gatgtataca
    38221 atacactcct atatccatta aagctccatt tgacaactct tttttaaagg cattttctat
    38281 aattccattt ttatatttat catatcttga agaatactga caatagcttc caaaaaatct
    38341 tcttattttc cctattttat ataagttttc tttaactgct ttaaagtttg gaacacatgt
    38401 aattctcata gcttccataa gcaatacatt attatctttg gctgttttta tcattgattt
    38461 gacttctttt aaattagatg ctaacgactt ttcacaaagc acatgttttt tgtgttttaa
    38521 acaagtaatg gtttgctttg aatgtaatgc atttggagaa gctatgtata cagcatctat
    38581 atccttcgat ttagccattt gttctaaatc atcaaaaaaa atacttgctc catatagagt
    38641 tccaaattct ctagcttttt ctaaatttct agaatataca gcaaccaatt caaagtcttt
    38701 aaccctagat gcagctctta gaaatatatt tgctatattg cttgtcccaa caataccaaa
    38761 tcttatttta cccatcataa atcactcccc attttctaca ctttatttta attttaataa
    38821 aaaactttac aacttataga tatattataa aataatttta tcaaataaaa aagaaagcta
    38881 attttaaatg atttcaccat tttaacatag ctttctttta actacaatct aaattcaact
    38941 tatattttta aagttatctt tctactatat ctttatttcc cctcaaaaat tttatagtaa
    39001 gaatttcatc cacaaacaat gttactaaaa gtatagttac aacaattggc caataaggta
    39061 ttataagact atataaacta tcaactatat acacttgaaa atatctaact ccaataaatg
    39121 tcaatatagt ccaatataca gaaaacttta gacatactat tcctctacta ttgtacttat
    39181 aatctgaata atcccaatag accttattaa aatatacttt cataagtatt cctgtaatat
    39241 attctactac tgtcggcact actagacata ataaaagcaa tgcaaatata ttattggggt
    39301 caataaatct tgagacctct attataatcg acattgcaat tgcatacatt ggtttaaatg
    39361 gaccatttaa aaatccatct tcttgaaaat gcccttgttt ataataacta tataagtttt
    39421 caattatcca acctataaaa ccatacaaga aaaaattaaa taaaaaatat aatagagaac
    39481 ctacattatc catgaatatt cctcctagct gtttatactt attttttctc atattcatgg
    39541 atttattcta aatcaaagaa attatattta atttatttaa ttaatacttt atattataga
    39601 ttgtgacatt ctttagagtt taaacttttt aggtattctc taacttccag taaattttca
    39661 agtatcttat gtgattgtga tttaatttgc tcatctggct cttttaaatc tggtacattt
    39721 atacatctta taccaccatt gtatgcagct tcgactccca taggcgaatc ctctatgaca
    39781 atacaatttt tgggattaac atttatcttt ttagctgctt ttaggaatat ctcagggttt
    39841 ggtttcgagt ttacaacatc atctccacaa actatagcat caaagtaatc ttttaaattt
    39901 gccttagcta atctctttac tgctctttct ctctttgttg atgttgcaac agccattttg
    39961 tatccatttt cttttaaaaa agagattagt tcattgactc ctaatttgat aggagctccc
    40021 ttcctctcca taaattcaat catattttta gttttttcgt catataaatc tattattggt
    40081 acagaactat cgtatatatc tgtaagacct tctattattc ctttacgatt tcttcccata
    40141 actgatgtat aaatttcctt agtcatagta tatccatact tttcaaacgt ctccatccaa
    40201 aattcaagag aaattctctc tgagtcaaac aaaactccat ccatgtcaaa aataattcct
    40261 tctacttttt gcatacaatt cctccaatat tttttgatat aattatatta cactttttat
    40321 gattaatttt acaaccctca atttatccaa ttctaatatt gtaaaattgt aattttcaaa
    40381 agttacactt tggtttactt taatatttga accaagcata taggatatcc acccacctat
    40441 actgtcaaat ccatcatgct ctatatcaag tccaaaatac ttattaatat agttgataga
    40501 ttcagttcca tcaactaagt aagaacctgc tcctattttc ttaatatgag tctcttcttt
    40561 gtcaaattca tcttgaattt ctccaactat ctcttctagt atatcttcta ttgttaccac
    40621 accagatgta ccaccatatt catccacaac tatagcaagt tgaagttttt cctttcttat
    40681 tctctcaaga gctttatcta tagttaaatt ttcagatata tatataatat ctctcaatat
    40741 ttcttctagt tcaattttat tttcatttat cttttgattg tataaatctc ttatatgtac
    40801 aaaacctaat atatcatctt tattttttct gcatactgga tatcttgtaa caccttcctc
    40861 ttttaaaata gctaatatct tttcttcaga atcactttca tatatacata ccatatcagt
    40921 tctaggaacc attatctctc taattttctt ttcttcaaat tcaaaaatat tatcaactaa
    40981 tctttgctct gattcatcta taagtccact tttataactt tcttctacta atagtcttat
    41041 ttcgtcacct gtatggggtt catcaacctc atcagcttgt gaatatccaa atggtttaag
    41101 aaataaatca gtacttaagt tgaaaaaata tattattgga tatgttaact tataaaatcc
    41161 tactaataaa aaagatgtac tcaacattat tttttctgta ttataaagtg ctaaagcttt
    41221 aggaaccaac tctcctatta caacctccat cattgttata attaaaaaag aaatggaaat
    41281 tgatatagta tatataatac tttcactaag attaaataca cttactattg gtaaaattaa
    41341 ctctttaact gtagattctc ccatccaacc taatgcaagt gaacaaagtg ttataccaag
    41401 ttgacatgca gaaagatagg aatttaagtt gtcttttatt attttacatc tttttgcatt
    41461 attattacct tcagctatca tggtatcaat cctagaacta cgaagtttta ccatagcaaa
    41521 ttcacaagct acaaaaaaag catttatcaa aacaagcatt actataagtg ttacttttaa
    41581 taactcccgc ataaagtatt ggttcttata atgtaagaaa tctataagaa ctcaaagttc
    41641 cccccttata tttataaatt taatttataa tatatattct atcatattta ttaatatatc
    41701 ctactttatt acaaataatt tttataaact aaataacccc tttaatattt atgactggct
    41761 atttgataaa tattaaaggg gttatttagt tatcattttt tatttctaaa aatcttttct
    41821 caaatgattt agttatctat ttatccaatt ttttacataa tttaatgatt aaaaatatta
    41881 attttttcac tataaagtac aaatattatc tgattattaa aataaatatt aatactataa
    41941 ataaaaacga agcatgattt ttatttaaat aaatctcaaa cttcgtcttt acttacttag
    42001 atatttattt actttctaaa tcctattatt tttaaaacct tatcttttca atacttcacc
    42061 acatttattc tcagtaatct ctccattttc taacgcaatt tttccattta caattacata
    42121 ttcaacacca ttggctagta attgtggctt tgaaaaatca atatcttctg acctaggata
    42181 actatagtta tccatgtcaa taactgctat atcagctatc aacccttctt taagaatacc
    42241 tctatttttt attttaaatt gcctagctgg taatgaagta actcttctta caatctcttc
    42301 caatctaaca ccagactttc tgtacatatt gaagaaaatt ggaaaagctc ctcttctttg
    42361 tagttcaaac catgacatat ccccacccac actaaacaaa caatctgaac caaccataac
    42421 aaatggatta tcaattattt tatcatcatg tagtttatct gggaaatcag ctctatacat
    42481 tcctcctaaa caaaagataa gttcttcgtc tccatctaaa agtaaatcga gtaataactt
    42541 gtcttcatct attccttttt gtgtagctat ttcacgtatt gatttctttt ccatattaat
    42601 atctttagtg tttagaagta ttatactccc cctatctcca agtataaatg cttcttttaa
    42661 tataagtgtt cttccctctt catttctaag agcatccatt accccatcta tacctaattc
    42721 aaatagacta gatgatattg ccataataaa ttgaattact ctttttttct tcatacaatg
    42781 cccagaactt cttggtactg tgtcaaccat tatttcaaca ccattatacc tagcctttaa
    42841 tatatcatca aatgcttcta tttcaactgg acttaagtga gatacttgaa ccctagcacc
    42901 acttttttgc ccaacctcta tggcttcttt tactgcatta tatcttccta tgtaatctct
    42961 aatatgagat gtatatatac catcatactc tttaatctgc cttgcaatat caacaagttc
    43021 atcaatatca gcatacttac ttggtatata tgccaatcca gtagatattc caaaagcacc
    43081 acttttcatt ccatcattta ttatatctgt aatttcattt ttttcctttt ctgtaggagg
    43141 tctatcctta gaacctccca taacactcca tcttatagtc ccatgcccca acaagaatcc
    43201 catattaaca gaaataccac ttttagatat aaggtcacaa tattctgtaa aattattcca
    43261 acatttattt ttatctataa gatacttttt ttcttcttca agtaatagac catttttata
    43321 catatattca tacacatttt cagaagaata tggtgttata gaatgtccac aattaccatt
    43381 tatagttgtt gtaactcctt gttttagaaa cttttcaaat ttaccatcta gtattgctac
    43441 tatttcttca tgaacatgag ggtcaataaa tccaggtgtt ataacttttc cacaacaatc
    43501 aatctcttta tttgtattgc tatttatctt agaatctatt tttgctatta agttgtcttt
    43561 aattgctata tctccataaa aaccaggatt acctgtacca tctattataa atccattttt
    43621 taagattaaa tcatatatca aaatattccc tccttaaaaa ccaattttta aataatctta
    43681 atgtaatttt tatatttgaa aatcaaatta ttatatttca tatttgaaaa ttcactatta
    43741 tttcattgta attagtcact aagcttatct ttatacatag ctcttgcaat taatatagta
    43801 ccaaatacac atataagaac tactagagac tgtggtatta taccttgctt tgataatata
    43861 atagtcaata cagatattac aattgctact agacctaatt ttttatcttg taaaaatact
    43921 tgtccaaaaa ctgacccaaa tagagctggt aaaattaagt ttaatttttc tactacttca
    43981 gctggaattt tagataatac gcttcctcct aaaattaccc ctatagttag tatacttata
    44041 ttcactaaaa tagatacagc tattcctatt gtagatatta tcgagccttc ttctgtacct
    44101 gattcaactt ctgctgcctt ttgagctgct attgaacatg gtagcctcat attagatata
    44161 ttaccagaaa gaaaactcat ataagttcct gggataccta agattggtga ataagaaact
    44221 ggttctatta tgtaatttgg agcactcata ctgacaattg ccattgtact tgctaataat
    44281 gctgatattg gaggcataat accaaatcca aagctcaaaa ttattccagg aactaatgct
    44341 gctataatac ccaaagatag tgttagtctt ccatacctta tcataaaagg taaatattct
    44401 tcattgtata tagattttac tttactcata aaactccccc cttatattac tgaagctatt
    44461 atcattccgc taaacattga tattgtaagc ccccactctc ttaaccattt tatatttttc
    44521 ttcttttcaa gcatacacaa tattaccatt atagccattc cacttataca tgcaattgtt
    44581 ccattatcaa aacgcaacac cctatcagca tttagatatg caaatgaacc caacattgct
    44641 cctagagata ttattggtat aaatgatttc ttgccactag atataacatt ggttatctta
    44701 tctagtttat gtccaaatag taatgtgaat attatccatc ctaaagaacc taatatcata
    44761 gtccaaactg catttgtaaa tgcaagtttt gtcatttcag gagaacctaa agttatacct
    44821 aatgcatctg ttccaaatcc ggctgacatt gattcaaaaa ttacagaacc tataaatgat
    44881 aatctcatcc atgctatagg cccacccata gttactataa gcgataccat tccagcaaat
    44941 atagtcattg aaggcccaat agctgaaata gcactactct taacagctga ctttatttgt
    45001 ttatctgtta ttcccattgt tttaccaact ttgcaagatt ttatagcaaa tataagggat
    45061 tgtaccacta ctattgatac tgctactgta ctcgctatcc acaaaagtgg atgattagct
    45121 aattttaaat aatccattta taaaacctcc ctataaatcc tcaaatttta ttaatatttc
    45181 taatatatct atttccaaac ctgttttagt actctaagta tatttccacc tagtaccttc
    45241 tctatttcct catcactata tccatgcttt actaaatatc taacaatgtt ttttgaagtt
    45301 tctgttgggt tctcaagacc ttctacatat tctactttgg gatgttcttt tgttcctgtt
    45361 atttttttta tatcaaattt agcagctagt actgagtgta attttacatg gtcgccatat
    45421 actgtatctg gtccaaaagc tacatggtct attcctacta aatttttaat atactcaaaa
    45481 tgttccataa atgactctat tgaatgttct ttattcttct ctgttattgt agtatgaggt
    45541 gctgcttcta cacctataac ccctcctttt ttagcacatg ctattaatac ttcatctgga
    45601 gttagtctct ttgtgttcca taaacttctt gccccaacat ggcttaatat tattgggtcc
    45661 ttgctatatt ctattacatc aagtgtagtt tgaactccaa cgtgagagca atctattgcc
    45721 attccaattt tattcattct ttcaacagct tttttcccaa aatttgtaag acctccatct
    45781 ttctcttctt ttagtcctga acctaatgca ttggattcgc tgtatgttat acccattaac
    45841 cttataccaa atccatacag tatatctatt ctgtcaagtt cattttctat tggtgctgca
    45901 ccttctaatg ttggtatcaa tgctacttta ccttcacttt tagctctgta tatatcttct
    45961 actttttcac actttatgac aaaatcttga tgagctaaat cacatagtct catgcctaaa
    46021 tctattaata tatcatccca tttccaccct gaatttgatg ttatcgtaca tacaccatcc
    46081 attaaattat caaatatagc atcataatat cctcttgata atgcttcata agcgcatctt
    46141 tgtttacctt ccctattata ttcaaatata tctctttcta ggtgttctgg aaataatact
    46201 ggatgttcat gtaatgatat aaatattttt tcatccacta tacttttaac cttcgcttct
    46261 tgaatatcat ctaattcaat aaggtactct ttaactcttt tatctccttt acacatctca
    46321 atagatgaaa tatcttttcc tttttctaaa taactatagg cttcatatcc tttgtaagct
    46381 gacttcataa caacatcctc caagttctac aattttttat atttttccta tgtagcaatt
    46441 gatgtgccaa aatataaatt ttaattgtat aataaatctt gcaaaaaata aaaacagtag
    46501 taaggttgat atttactact gttttattaa aaataatctt cttagattat ttattcaata
    46561 tttttgaatt atcttaatct taaagataat tttatttatt tctatccatt atagggattt
    46621 ttttagggtg ttttgccttt ttagtcccct atatcactcc cttaattttt acatatgata
    46681 taaagttctt tccttcatca gttataaaac taccttgttt ttttactcca gataaaagta
    46741 aaccatggct ctttaactta tctattcttt ttctaatttg gtctacactt aaaactatat
    46801 ctttttcttt taaaatctct tgcagtttat ttcttcctaa taatatattt ttattattcc
    46861 aagtttctat actggtcaat atacatatac tttcctcaaa gaaattagat tgtttgaaat
    46921 ctaagataat tgaatcgaaa ttttcattaa ctaaagtatt gtttttttca aatctaaatt
    46981 gttctggcaa atgctcgtaa tctactctat cttcctcaac tatagtatca atataataaa
    47041 ttaaattttt aagctctctt acatttcctg gccattcata caatgttaaa gcttgcattg
    47101 attcctctgt aaaacatttg ttgctattta tttcatctaa aaaatactta cttataagag
    47161 gtatatcttc cttacgttct ctaagtctag gtatctctat atgtaataca ttaatcctat
    47221 aatacaaatc ttctctaaag gaaccttctt taattttttt ctttaaatct ttatttgttg
    47281 cagctataat tctaacatct attggtataa ttttacttcc gcccattctc ataacttctt
    47341 tttcctgtaa tactcttagc aatctctgct gtacatctaa agatatatca cctatctcat
    47401 ctaaaaaaat tgttcctgtg tgtgctcttt caaatatacc cattttccca ccttttattg
    47461 ctcctgtaaa acttccttca tcatagccaa aaagttcact ttcaataagt gtatctgaca
    47521 aagaagatag attaactgca acaaatggct tgttttttct tagagattca ttatgtattg
    47581 cctgggcaaa tatttctttt cctgttccat tttctccaag tatcaacaca gaaaagtctg
    47641 tagttgctat ttttcttgca atattaattt tttcttttat tattttactc tcacctacaa
    47701 cactctcaaa tgtatacttt gatacaaaac cttttgactg aagtttattt tgaatcttgt
    47761 cctcaagctc ttgaattgca cttatatttt taatgcttat aatactcttt attcgttcat
    47821 tattttcata aacatttact ttattaatta tcagtttttt gttatttaaa tttactactt
    47881 catcattaac ttcatcttct tgaaaaaata tttttttgac aaccttatca ctaaataagt
    47941 ccataaagtt attagatata atttcatttt ggtctattcc aactagatta gaaaatactt
    48001 tgttgcaata tataaatttt cctaatttat caacagaagc aataccttca tctataatct
    48061 ccaaaaaact cttcatggtt ttattcatag ttgtataata cctatatcca cttactgtat
    48121 cttcattgta tttttcctta ataatatgta atttatctat agatatattt agttttgtaa
    48181 agatttctat aactgtattt atatcaatta ccttatcacc tatgtcaatt atttgcttta
    48241 tgttttgtgg tatgctgttt ctgcttcctg taataattcc aatctcacat ttagacttat
    48301 cacaacctgg ataatatggt attagattaa tatgaattaa tcctagtttt ctaattatct
    48361 tggcagtttc atctgctgac tctttataag catctataac cataacatca gaatcatttt
    48421 ctatacttat tatctgattt atattttcaa tatttatagt acgatgtact attacaatag
    48481 gtatattttt atcaatatta ttatgtatat attcctttat atcattgtct gaacatacta
    48541 caagctcata gcttgacagt tcaatattta tcctatttac atataccaag ttatctatat
    48601 tataaaattc tccaaatata ttttccaatt gttttgatat atctatattt attttgtcat
    48661 cagttgatac aaataaaata ttatgttttt tcatagtccc acctcctgtt taacgagtat
    48721 agaaagtaat aatttggtct attgtagcac tttttaaaaa agttttaaat cttaaaaatc
    48781 aaattttgta ccatttacta ttattttcca taataaaata atgtaagcta tttttttcac
    48841 atataattta atttctaaaa cggttagttt gacattattg gaatataaac aaaaatttaa
    48901 tataaattcg ttaatatatt aatacatttt taagaatgat aaggaataaa ttataacaaa
    48961 ttttaggaat atataataat agggtctcag aacttagtta caagttctaa aaccctattt
    49021 gtttagtgta aaaaacatca taaaatttag cttaaatctt ttcacataat attactcttt
    49081 ttctaagtga taataaaata tatctattcc tctaaaaact gtaaaaattc ctgattaaat
    49141 agtttaagct catcatagaa tatagcatga ccactatact caaaagtata taaaattgaa
    49201 tcttctattt tttcattcat aaaaactgca aattcatacg gacatatttc atctaatttc
    49261 ccatgaaata taccagtagg cactcttaca cattttaaat cattaaataa ttcttcatct
    49321 cttaaagata cagcagttcc aatagtgcca atacctgatg cactaaaacc aatatcatta
    49381 aaccatcttc taaagctttc tggtggattt gatgcaaata ctttttctcc aaaatcggtt
    49441 accatttctg gtctatcagt acaggcttgt gctatcaatt tatttacatc ttcacgtgtc
    49501 ataccatatg gaaattctgg aggtctttgt acaaatgatg gtgcagcagc tgcagctaat
    49561 accaattttg acactccata tccattaaat aaactcatat acctaagtac gattgctcct
    49621 cccattgaaa aaccaacaag ggtaaagtct tttaatccaa tagcatgtac caccttataa
    49681 atatcgtctg ctaattggct ataagtatat cctcctgatg ttgcatcaga tttaccaaaa
    49741 cctcttaaat caattgaaac tgttctatat ccgagttttg gcaatatatt tgtttgatat
    49801 tcaaatatct tatgtcctaa gggccaacca tgtataaaaa gtacagtttt tttagcactc
    49861 ggattcaaat catatactgc tatgtttata tcattcacat atacataata catatcaaca
    49921 gcaactccct tcaagaaaat gtatgatggt tattttatat atatgaataa aatgatgaat
    49981 aactaaattt tcaaatttat ttacaatact tctctattgc cttttcaaat aaacttacat
    50041 gaagtttttc atctaggatt attctattta atatagcttt tatataaggg tcttcaataa
    50101 gacttatatc tttataataa ttattaatag ccacatgctc caaatgcaaa tctagtttta
    50161 atctgttaca aatagaattt ccataacata caaaaccacc attccaatat gcaccacaag
    50221 aacttgtaga acctctatat acaggatttc cacccaataa tcttatagtc tttgctaata
    50281 tctccatatg taacatttct gttatagata tatttatcca catttcactt aactctctat
    50341 gcgtttcatc taaatcaaaa ttatggtata gatattgatt tacactcgta aattcacttg
    50401 atactccggc atagtcatcc atcaataatt ctgcatagta cttatttgga cctaaaactt
    50461 ttatctctgg atatggctca tcacttgaat atccatttcg cttgtgcatg tcataatgtg
    50521 catcactcat aatatctcct cctttaaatt taatgtatga tattaattgt atgcatcacg
    50581 actttttcat ataacttaga taataaaatc tacttaaata ataaaaagcc atattaatta
    50641 aaattaacat gaccttttat ccacaaaatt actttttaat tattcatctt ttccagctac
    50701 tgatagctct ttaataacaa ctgaaggcga acctatacta ctcattggaa attctaaatc
    50761 tgctcctata gcttcaatat cttttaacag gtcaaaatag tttcctgcta ctgtaatttg
    50821 ctctactgga aatacttttt ttccttcttt gatataaaag cctttggcag ctaaagaaaa
    50881 gtctcctgtt actgaatttg cacctgaatg aagtcctgca aagctagtaa ccatcaatcc
    50941 ttcatctata tcctttataa tttcatctaa agatttatct cctttctcta tataaaaatt
    51001 agttggatat atgcttatag gtgatgaata tgaagatttt attccatttc ctgtagtctt
    51061 tacacctgcc ttattagccg tttttaagtt atgaagtaaa gttataagct ttccatttaa
    51121 aacaacttct tttttaaatg tagctactcc ttcatcatca aaaggagtag atgccatacc
    51181 attttctaaa agtgggtcat caactattgt aactatagga gatgctatca tgtctccttc
    51241 tctatttttc aataaagata atcctttttg agctgaatct gcattaaata tcccagaaaa
    51301 tgtgctaagc aaagatacca tagcttcatt aagcaatata gttttatatt tacctgatgg
    51361 aatactctta ccacctactt tagataaagc attatcaaca ccaagttttg ctatctcaca
    51421 aggttttatt tcttcaatag aatttgctat attatatcct attccatcat atttttgtcc
    51481 attgtcttct atgataggaa ctacaaaccc tataagcata ttagttttat tacttaaatt
    51541 aagaccttta gtattagata taccattact tgatacagaa taagatatag tacatttaga
    51601 taaatttacc accttatctg aataagcttt agtctctctt tccatttcta aagctaaatc
    51661 aatcaactta tctgcctcta aattttctag ctctttagaa taagtcttta catcattata
    51721 gtgcttatct ccttcatata taaattgtac atcttcattt tcaatagtgt ttactccatc
    51781 ttttacattt tttatcaaca tatcaacagc tttatcatct aaaatttctg tataagaata
    51841 acctatcttt ccatttacct tacctctaaa agataaacca aaagatttat caagattgta
    51901 tttctcaact tctccttcat atatatttat acttaaattt tctcctgtat aataatatat
    51961 ttcacactct tcaaaacctt cacttagagc ctttttaaat aatatatctt taaaattttt
    52021 aagttccaca attatccctc ctatcttcca cctacagtaa tttcttttac ccttatcata
    52081 ggttggccta cattagttgg tatacttcct gatgatgagc cacacatccc ctgagcttgt
    52141 tttacgttat tcccaaccat atctatattc ataagtatat cgctaccttt tccaattaaa
    52201 ctagcacctc taactggctc ttgtatttct ccatttttta ttaagtaacc ttctgaaact
    52261 acaaaattaa actctcctgt tacaggattt acagaaccac cacccatttt tttagcataa
    52321 agtccatctg gtattgactt tataatatct tctggtttat cctctccagc agcaatataa
    52381 gtgttagtca tccttgaagt tggtgcaaat ttgtagttct gtctacgaga acttcctgta
    52441 ggattcattc ccattcgtct accattaagt ttatctatca tataagactt caaaattcca
    52501 ttttcaatca gtatattctt ttgagttgga tttccttcat catctatatt catagaaccc
    52561 cagtagtttg gtattgtacc atcatctata gctgtaatct ttgtggaagc aatctgttga
    52621 cctaatttat ctgcaaatac agagttcccc tttgcaactg atgtagcttc caaagaatgt
    52681 ccacaagctt catgaaatat tactccacca aatccattat ctattgctac catcatatta
    52741 cctgcaggac aatttttagc atgcaacata gtatgcgcta ctctagctga ttcttttgca
    52801 taatattctg ggtctatttc ttcaaatatt tcaaaccctt tacaacctcc aggaccttca
    52861 aatccagttt gattttcatt acccttagat gcaattgaac ttatccctag tctagttctt
    52921 attcttctat cctcaacata tataccttca gtgtttgcaa ttaatatttt ttggtcttta
    52981 tccaaataac taacacttac ttgagatata tcattactaa attcttttgc actcttatat
    53041 gcaaccttca taacacttac tttatcttta tttcctattg aatttggata aatttttata
    53101 ttatgtatgt tatttatttt aatactgtca tttaatatta cactttttcc atcttccaat
    53161 ttacctaaag caacagcagc tttgtatgct acatccaaca gactatttaa tgaattgtca
    53221 cttgtataag catatatact tttcaatcct ttaaatattc ttattcctat accataattt
    53281 cttccaccta ttgcattttc tactttatta tctattaaac taatagaatt atttatagtg
    53341 tcttcttcaa aaatctcagc aaaatctcct ccagtaatta gacatttttc taatacctta
    53401 gaagctatat tttttgaaag cataaattta cctcctactt taaattttta attaactttt
    53461 attcaatctt tttacttgtt aacaaacatt atatactata ttacttaaaa taaggtaatt
    53521 atgaatatat tatacatatg
//
LOCUS       DAENCV010000010        52477 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAENCV010000010 DAENCV010000000
VERSION     DAENCV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.010"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            135..485
                     /locus_tag="KQD82_000529"
     CDS             135..485
                     /locus_tag="KQD82_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG4379033.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            504..2147
                     /locus_tag="KQD82_000530"
     CDS             504..2147
                     /locus_tag="KQD82_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG4379034.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2228..4285
                     /gene="recG"
                     /locus_tag="KQD82_000531"
     CDS             2228..4285
                     /gene="recG"
                     /locus_tag="KQD82_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG4379035.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4344..4898
                     /gene="rsmD"
                     /locus_tag="KQD82_000532"
     CDS             4344..4898
                     /gene="rsmD"
                     /locus_tag="KQD82_000532"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG4379036.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4900..5397
                     /gene="coaD"
                     /locus_tag="KQD82_000533"
     CDS             4900..5397
                     /gene="coaD"
                     /locus_tag="KQD82_000533"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG4379037.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5401..5925
                     /locus_tag="KQD82_000534"
     CDS             5401..5925
                     /locus_tag="KQD82_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379038.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6269..8188
                     /locus_tag="KQD82_000535"
     CDS             6269..8188
                     /locus_tag="KQD82_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4379039.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8201..8488
                     /locus_tag="KQD82_000536"
     CDS             8201..8488
                     /locus_tag="KQD82_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4379040.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8621..9880
                     /locus_tag="KQD82_000537"
     CDS             8621..9880
                     /locus_tag="KQD82_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG4379041.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9883..10623
                     /locus_tag="KQD82_000538"
     CDS             9883..10623
                     /locus_tag="KQD82_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG4379042.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10946..12256
                     /locus_tag="KQD82_000539"
     CDS             10946..12256
                     /locus_tag="KQD82_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG4379043.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12354..13271
                     /locus_tag="KQD82_000540"
     CDS             12354..13271
                     /locus_tag="KQD82_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG4379044.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13287..14096
                     /locus_tag="KQD82_000541"
     CDS             13287..14096
                     /locus_tag="KQD82_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG4379045.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14154..15902
                     /locus_tag="KQD82_000542"
     CDS             14154..15902
                     /locus_tag="KQD82_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG4379046.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16164..17186
                     /locus_tag="KQD82_000543"
     CDS             16164..17186
                     /locus_tag="KQD82_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG4379047.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17191..19497
                     /locus_tag="KQD82_000544"
     CDS             17191..19497
                     /locus_tag="KQD82_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG4379048.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19469..20584
                     /locus_tag="KQD82_000545"
     CDS             19469..20584
                     /locus_tag="KQD82_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379049.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20602..22020
                     /locus_tag="KQD82_000546"
     CDS             20602..22020
                     /locus_tag="KQD82_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG4379050.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22035..23243
                     /locus_tag="KQD82_000547"
     CDS             22035..23243
                     /locus_tag="KQD82_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379051.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23317..24705)
                     /locus_tag="KQD82_000548"
     CDS             complement(23317..24705)
                     /locus_tag="KQD82_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG4379052.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25046..26380
                     /locus_tag="KQD82_000549"
     CDS             25046..26380
                     /locus_tag="KQD82_000549"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG4379053.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPSIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNISK"
     gene            complement(26522..27439)
                     /locus_tag="KQD82_000550"
     CDS             complement(26522..27439)
                     /locus_tag="KQD82_000550"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG4379054.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27647..28531
                     /locus_tag="KQD82_000551"
     CDS             27647..28531
                     /locus_tag="KQD82_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG4379055.1"
                     /translation="MKVLKKITILLLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28744..30585
                     /locus_tag="KQD82_000552"
     CDS             28744..30585
                     /locus_tag="KQD82_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG4379056.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30890..31597
                     /locus_tag="KQD82_000553"
     CDS             30890..31597
                     /locus_tag="KQD82_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379057.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31591..32901
                     /locus_tag="KQD82_000554"
     CDS             31591..32901
                     /locus_tag="KQD82_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379058.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33018..33701
                     /locus_tag="KQD82_000555"
     CDS             33018..33701
                     /locus_tag="KQD82_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379059.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33694..36081
                     /locus_tag="KQD82_000556"
     CDS             33694..36081
                     /locus_tag="KQD82_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4379060.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36361..37503
                     /locus_tag="KQD82_000557"
     CDS             36361..37503
                     /locus_tag="KQD82_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG4379061.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37522..38046
                     /locus_tag="KQD82_000558"
     CDS             37522..38046
                     /locus_tag="KQD82_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG4379062.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIIFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKNCIK"
     gene            38146..38964
                     /locus_tag="KQD82_000559"
     CDS             38146..38964
                     /locus_tag="KQD82_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4379063.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEVVSQLFAKKLEESLSPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            39042..39353
                     /locus_tag="KQD82_000560"
     CDS             39042..39353
                     /locus_tag="KQD82_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG4379064.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39530..40213)
                     /locus_tag="KQD82_000561"
     CDS             complement(39530..40213)
                     /locus_tag="KQD82_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379065.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIHNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40306..40947)
                     /locus_tag="KQD82_000562"
     CDS             complement(40306..40947)
                     /locus_tag="KQD82_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4379066.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41121..43124)
                     /locus_tag="KQD82_000563"
     CDS             complement(41121..43124)
                     /locus_tag="KQD82_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG4379067.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43277..43915)
                     /gene="recX"
                     /locus_tag="KQD82_000564"
     CDS             complement(43277..43915)
                     /gene="recX"
                     /locus_tag="KQD82_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG4379068.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            44191..44880
                     /locus_tag="KQD82_000565"
     CDS             44191..44880
                     /locus_tag="KQD82_000565"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG4379069.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            44881..45453
                     /gene="yqeK"
                     /locus_tag="KQD82_000566"
     CDS             44881..45453
                     /gene="yqeK"
                     /locus_tag="KQD82_000566"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG4379070.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            45570..45920
                     /gene="rsfS"
                     /locus_tag="KQD82_000567"
     CDS             45570..45920
                     /gene="rsfS"
                     /locus_tag="KQD82_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG4379071.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            46274..48694
                     /locus_tag="KQD82_000568"
     CDS             46274..48694
                     /locus_tag="KQD82_000568"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG4379072.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVETFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            49530..50198
                     /locus_tag="KQD82_000569"
     CDS             49530..50198
                     /locus_tag="KQD82_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG4379073.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            50238..50318
                     /locus_tag="KQD82_000570"
                     /pseudo
     CDS             50238..50318
                     /locus_tag="KQD82_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(50681..50953)
                     /locus_tag="KQD82_000571"
     CDS             complement(50681..50953)
                     /locus_tag="KQD82_000571"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4379074.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(51184..51605)
                     /locus_tag="KQD82_000572"
                     /pseudo
     CDS             complement(51184..51605)
                     /locus_tag="KQD82_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(51624..51722)
                     /locus_tag="KQD82_000573"
                     /pseudo
     CDS             complement(51624..51722)
                     /locus_tag="KQD82_000573"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            52005..>52477
                     /locus_tag="KQD82_000574"
     CDS             52005..>52477
                     /locus_tag="KQD82_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG4379075.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNA"
BASE COUNT        20334 a         5818 c         9052 g        17273 t
ORIGIN      
        1 aaaacttttc taggtttaaa ttataccata tttatagtat gatatgatta taattttata
       61 taataaataa gtaaaaatag taaaaaataa attaagaaat gtttttaata aaaataagct
      121 taagggggtc aaccatgagt gcaaaggtaa cgaatcaata tggaaatata gaaatagata
      181 atcaagtaat agctcaagtc gtatatagag ctgcaatgga aagttatggc ttagtaggtt
      241 tagcatataa gtctaaagga atagtagagc tattaaaagg cgagaacgca actaaaggtg
      301 taagagttga ggaagtagaa gaagatgcca tagcaatcga actttatgtt ataatacaat
      361 atggtacaaa tatatctact gttgccaata atataataga tagagtaaaa tatgtagttg
      421 aaaaaatgac tggcgtaaga gttactagaa ttgatattaa tgtacaagga ataagagtta
      481 agtaatttca taggaggaaa caaatgattc aatatataga tggtaaaagg ttaagggaga
      541 tgtttatttc aggtgcaaac aatctccaaa acaataaaga gttagttgac aagttaaatg
      601 tattccctgt accagatgga gatacaggaa ctaatatgtc attaacaata tcttatgcct
      661 taaaagaatt agcaaaagtt gaaaatgata atatatcaga tatcggaaaa gcattatcta
      721 aaggttcttt aatgggagct agaggaaact caggagttat actttcacaa ataatcagag
      781 gtatagctaa atctatagaa ggaaaaagta aattatcaac agaagattta gcaaaagctt
      841 ttaaaaatgg ttcagacaca gcatataaag ctgtaataaa accaattgaa ggaactatac
      901 ttactgtagt tagagaaagt ggagagtttg caataaaaac tgcgaaaaaa gagaaagatg
      961 tagtaaagtt tttaagtatg cttgtgaagg aatcaaattc ttcattagaa agaactccag
     1021 accttctaaa gaatctaaaa gaagctggtg tggttgactc tggtggaaag ggtcttgtac
     1081 ttatatatga aggaatgtta gcttctataa aaggcgataa catagaaata aaaaatgttg
     1141 acctagatac taatatttca acaagtatgg attttacaaa gagtactact agcacagatg
     1201 atataaaata ttgctattgt acagaattta ttttagaaag ttctaaggta gaagatacta
     1261 aaataagaga cataatgatg gcttatggtg atagcctagc agtagttgga gatgatggag
     1321 tcataaaggt acatgtacac actaatgacc caggaaatgt acttcaagaa gctctaaagt
     1381 acggtcaatt attaactata aaaatagaaa atatgaagtt acaacatgaa aatacattac
     1441 tggatgtaga agaaaagaaa gaaaatgata gtgaaccttt agaagaagaa aaagagtttg
     1501 ggtttatagc tacttctatg ggtgaaggtt tggcaaatat atttaaagat ttcggagttg
     1561 accatataat agaaggtggt caaactatga atccaagtac tgaagatttt atgaatgcta
     1621 taaagcatat aaatgctaaa aatatattta ttttcccaaa taacagcaat ataataatgg
     1681 ctgctaatca ggcaaaagaa ttgagtgata aaaatataat agttatacct acaaaaaata
     1741 cgcctcaagg atttgctgca ttagtaactt ttaatggaga attaagtgaa gatgaaaata
     1801 aagaagctat gatgaatgct ttaaactcag ttaaatcagg tcaagtaact tttgctgtta
     1861 gggatacagt gatgaacgaa attgatgtta aagaaggtaa tataattgga attgcagaag
     1921 ggaatttatt atcagcagga gattatgtgg atgaagtaac atcaaacctt attgaaaagt
     1981 tggttgatga agatactgct ataataacat tattctttgg tgaagatgtt acagaatctc
     2041 aagcaaatga actacgtact tctttagaag aaaagtttga agatgtagat gtagagttat
     2101 attatggagg acaacctctt tattattact taatttcagt tgaataatga ttataagaac
     2161 ctgtatgacc aagtagcttt tataaaattg gttgtacagg atttttgtgt agtaggtgat
     2221 atatttgttg gatttatata aagatgttca atatgttaag ggaataggac ctaaaaaagc
     2281 agataaatta aataaactgg gtatatttac actaaaagac ctattgtatt attttccaag
     2341 acaatttgaa gatagaaata atctaaagaa aatagcacaa ttagaagatg gtgagaaagt
     2401 tacgataaaa gcagtaatat caagtataaa tacatttagc ccaaaagaag gtatgacttt
     2461 aacaaagatt gatgtaaaag atgagacagg ctcggctaaa ttagtgtttt ttaataagag
     2521 ttatataaaa aatacttttc gtccaggaga ttcaatatta gtttttggga aggttaagaa
     2581 aaaatttaat aacttagaac ttacatcatg tgaattagaa tatcttacta attcacctaa
     2641 gaatacatgt agatttatgc ctgtgtacca gcttacatac ggtgttacaa ataaagaaat
     2701 tatgagtata attagaacag tacttgaaga taaagaatta attatacaag aatatatgcc
     2761 acaaagaatt atagaaaaat ataggctatg tagtatagat tttgcagtta gaaacataca
     2821 ttcaccaagt agtaaggaat cattaaaaat agctttatat agaattgtat ttgaagaatt
     2881 acttatacta caactagggt tgtttgtatt taaaagtggc agaaataaag aagatggaat
     2941 aaagtttgaa acaagtaaag acttaaaaaa aataataagt gctttaccat ttaaactgac
     3001 taaagctcaa aacagggcat tagatgaaat tatacaagat atgaatttag agaaaataat
     3061 gaatagattg gtacaaggtg atgtagggag tggtaaaaca gtagtagctc tcttagcact
     3121 tgcaaattgt gttttaaatg gttatcaggg cgctttgatg gcacctacag agatattagc
     3181 agggcaacat tatatttcac ttactgagtc tctaaaagat tttggaataa atgtaggtct
     3241 attaataggt agtcttacaa aaaaacaaaa ggatacagta ttggaacaaa taaagaataa
     3301 tgagattgat atattaatag gtacacatgc actaattgaa gataaggttg aatttaataa
     3361 tattggttta gttataacag atgaacaaca tagatttggt gttatgcaaa ggagcaaact
     3421 ttcattgaaa ggagcaaatc ctgatatatt agtaatgaca gcaacaccaa taccaagaac
     3481 tttagcactt atactctatg gagatttaga tatttctatt atagatgaac ttcctccagg
     3541 aagacagcca atagaaacaa ttgctattga aaaaagtaag cgagatagag catataataa
     3601 ccttgtaaga agagaggttg aatcaggaag acaagtgtac attgtatgtc ccttggtaga
     3661 agaaagtgaa gccatagaag ccaaatcagc agttgaactt gttgaggagt taagagcaga
     3721 atattttcac gacttaaggc ttgggttgct tcatggtaaa atgaagtcaa gtgaaaaaga
     3781 tgaagtaatg agacttttta aaaataaaga aatagatatt ttagtttcaa caacagtaat
     3841 agaagttggt gtcaatgtgc caaatgcaac attaatgata atagaaaatg cagaaagatt
     3901 tggacttgca caattacatc agcttagagg gcgagttgga agaggtagtc ataaatcgta
     3961 ttgtgtacta atttatgatt ctaaaacaga tgtatgtaga caaagaatgg ctataatgga
     4021 agagaccaat gatggtttta agatttcaga gaaggattta gaaataagag gacctgggga
     4081 attttttggg acaaggcagc atggtcttcc agaacttaag gtagcaaact tgtttaagca
     4141 tataaaaata ctgaagttag cacagcaaga agctcgatat atattgggtg aagataataa
     4201 tctacaatta aaagaaaaca tggcactcaa aaaagaaatt atagataaat ttaaagatac
     4261 attgaaagaa atttcattaa attaatttta aattgtgtta aaatattact aatgtaataa
     4321 tgtgctgaag ggagattgtc atattgagag taatttcagg aaaagcgaga ggattaaaac
     4381 taaatactcc aaaaaatgaa gatgtaaggc caactacaga tagagtaaaa gagtctctat
     4441 ttaatatgat aaattcatat ataatggaaa gtgaagttct ggatttattt gctgggactg
     4501 gttctttagg aatagagtgc ttatctaggg gtgccaagtc atgtactttt gtagatatca
     4561 gtaaggaaag catagatatt gttaagtcca atataaaaaa ggcaagagtt gaaagtgaaa
     4621 gtattatttt aaaccttgat tttaagactg caatagataa acttaaatta caaaatagta
     4681 aatttgacat aatttttatg gaccctccgt attataaaaa tatgtttata gaagctattg
     4741 aaaaaattga taattctaat ctattgaacg aagatggaat aatagttgta gaacatgata
     4801 cgaatgattt atttccagat aagatatgta aattggagaa aactagagat aagaaatatg
     4861 gaaatactac attaactttt tataagatgg aggcataata tggaaaataa acctagaaaa
     4921 gctatatttg caggaagttt tgacccaatt actaatggac atttagatat tatttgcagg
     4981 gcttcgaagt tatttgacga attacaaata ggagtattaa ataatcctaa taaaaagggt
     5041 ctatttagct ttgatgaaag agtaaaactt atagaaaaaa gtacaagcca tttaaataat
     5101 ataaaagttg tgacttttga tggtttatta ataaattact gtcaggaaaa tgggattgga
     5161 gcattagtta gaggtgtgag aagtggtgct gatgttgatt atgaacttca gatggcacat
     5221 atgaatagag agcttaatcc agatatagag actataattt taccaagttg tacaaaatat
     5281 tcatttataa gttcatcttt aataaaagag gttttgctct ttgatgctga tataaaaaat
     5341 ttggttccaa agattgtttt ggaagaatta aagaaaaaaa ctagtggggg taattgatat
     5401 atgaagatag atttagaaat gattgaacta tttgagcagt tagaggagat atttagaagt
     5461 gcttctacaa tccctttctc acataaatgc acagtggata aagatgaggt tttagcaata
     5521 attaatgata taagaacttt aataccagaa gaagtaacac aggcagtatg gattaataaa
     5581 gaaagaaata aaataataag tcaagctaag caagatgcta caaatatagt tgaacaagcg
     5641 aaaaaagaag ctgagagaat acaacaagaa tatcaagata atatagagga attaaagaaa
     5701 aattctgaag atgtagtaaa ggcatatgta gaatccagcg aacctgttgt tcaagcagac
     5761 caaagagcaa aagatatagt tgatagagct gagagaatag ccaatgagat aagaattggg
     5821 tcaattgaat atgcagagga tgtgctttct agtgttgagt ataatttaag agaaatatta
     5881 gaagaagtaa aaagagataa agctgaactt agacctaaaa aataaatttt aaataaataa
     5941 aaatggagct atcacacaaa aaattccaat ttatgtgtga tagctctctt ttaatatagc
     6001 aattttttgt tatataattg tgctttttag attacttata tcttattttg atattataat
     6061 gtagtttaat cttttatttg ttgaaatcaa cttatttatt tgtataaata atataggtta
     6121 ttttcaaatt tttttaataa ttttaccact gtcttctttt agattagagt tagactgtgc
     6181 aaaattataa aataatacta aatcatgtgc aattatttgg tatggcacta catgtagaaa
     6241 atatagataa taaaattaag gaggtagtat gaaggaaagg gctaaaaaaa tattagattt
     6301 tattattaaa aataacaatg taacatcaca agaactacaa gaaaagttta atgtttcaaa
     6361 gagaactatt tattacgata ttctagctat aaataaacaa ctaggaaaaa gtggtaatat
     6421 aaaaaatgta aaacacaaat ttatatttga aggtaattta tgtgatgcaa ggaaaataat
     6481 ttcaactgaa gaagataaat ttttagatag tgattacaga aaaactttta tattaaacaa
     6541 gatattactt ggcgaaaaaa tatcaataga aaaactaaca aatgaaatgt tactttcaaa
     6601 aaacacagta gtacaaacaa taactgatgt aaaaaaatac cttcaaacaa tgggattaag
     6661 attagagtat aagggaaagt acaaaattat aggtgatgaa tacgttatta gagagctgtt
     6721 tctgataatt gttcaagaaa acgtactgga aattaattct ataagtgagg aagtaagtag
     6781 tttcgataca aaaggacata taaaacttac tgattattct ttattaaatc taactaagtt
     6841 tgtggaattt ctaaataaaa ggataagaga tgggaaaaca ttatactcat ataaatatct
     6901 aaatgaagct aaaaaaatta gttactttag taattgtaaa gaacttttat gtgaagaagc
     6961 taatgaaaat gaacaagcct atatatgcac ttacataagc tcacttccca gcttaaatag
     7021 tgaagttaag gaagatgttg ttgaagaata tgtagataaa cttatagata agtttgaagt
     7081 aaatacagca ataaaattag aaagtaagca tgagtttaag aaaaatattt taaggcacct
     7141 ccactcttct tacaacagaa tacgatttaa gtttcctatt agaaatccaa tgttagatga
     7201 gactaagtat aaacatgagt cactttataa aattataaaa tcaattatag agaatgaaga
     7261 agagtttcct gtatttgagg ggataagaga agaagaaata ggatttatag cagcgtattt
     7321 tggaggatat ttaagagggt ctagggataa tggtctaaga agaaataaag tcttactagt
     7381 ttgtcctaat ggtcttatgg tatcaaagag tttggaaata caactatata aatacatacc
     7441 aaccatagaa atagtaggta tagtatccat taagcaacta aaagaagtaa atgtatacta
     7501 cgattacata attacaacta tagatataca aaatgtaaac aacgtaattg ttgtaaatcc
     7561 tcttttaaca tcatcagatg ttcaattact tatgaacaaa ctaataagtg taaaagaaaa
     7621 cgagaagtat ttcaatcttg aattaataat acaagctata agaaaaaatg gagttatcaa
     7681 taatgaagaa gcactaaaag ctgatttatt aaacataata cacaagatag atgaaggaga
     7741 gatgtatcaa ccaatgttaa aggaattaat taatgcagaa agggtcaata taataaaaaa
     7801 tgttagagat tggaaagaag caataaaaat agcctcaaag ccattgttag aagataactc
     7861 tatagaagag ttatatatag aaaatatgat aaagtcagtt gagaaatatg gaccatatat
     7921 tgttttagct gatagatttg ctctaccaca tgcaagttct aaagaaggag taaacaagct
     7981 tgctatgtct ttactaatag tagaagatga agttgattta ttaggaaaac cagtaaatat
     8041 atttatggta ttagctgctg tagataacac tactcacatt agagcattag catcactatc
     8101 agagatgatg tacgaggaag aaaatgtaaa actaataatt aatggtgata aaagttcaat
     8161 actagaactt ataaataagc aaaattagga ggaatgaaaa atgaagatat tagcagtatg
     8221 tggttttggt gtaggttcat caatggtatt aaaaatgaca atacaaaagg ctctaaagca
     8281 gttaggtata gaatgtgaag tagaaaatac agatataaat tcagctagag gtgctgattg
     8341 tgactgtata ttcacaagtc aagaattgtt aaatgaatta aaaggaactt gtaatgtaga
     8401 tgtatatcca gttaaaaagt acatggattt agcagaagtc acagaagttg ttcaaacttt
     8461 tttaagtgat aaaaatgata agaaatagta taagttttaa aaagtaaatt taagttaatc
     8521 agaataaaat aaaaacttac taagttcaaa aatagtaaga aagagaggag ggatttagtg
     8581 gtagaaaact taaacagttg acttatcact aaaaaatata atgcttgatt ttatttcaaa
     8641 taatatttta cgtaatccat ctatgttaat tggattaata gcagcatttg gtcttgtact
     8701 acagaagaag aaagcatctg atgttataaa ggggtcacta cttgcagctt tcggaattat
     8761 aattatggag actggaacag gtatgttagt tagttcaata gcaccaatta atggagcttt
     8821 ccaaagtata agtggtggtg cagtaacaaa aggtttaagt gatgttactt ttactgcaag
     8881 ttatggtgga attataggtt tagcaatgtt tgcgggttta gctattcacc taatgatagc
     8941 aagatttact cctattaaga caatcttctt aacaggtcac atgctttatt ggttcccatt
     9001 catatttgta gcttcaggag ttgaagctgg attaaatggt ggagtattaa tagcttttgc
     9061 agcagtatta tcagcactct attggtcaat aatgccttgg ttattaagaa aatatgtgtt
     9121 tgatgttata ggagatgatt cttttacatt aggtcatcca tctggttgtt tagcacttat
     9181 atcaggattt gttgctaaat tagtaggaaa taaagaaaaa tctacagaag atataaacat
     9241 acctcaaagt ttatcatttt ttagagaaat atctataagt ggtggaatgg cagtgttttt
     9301 aatgtttata gttgttggga tagtaatacc aggtgcgttt aaagatagta atgagtttat
     9361 tggaacgtat gcaataaagc aaggtctttc ttttggagca ggtcttataa taatgcttca
     9421 tggggtaaga atgctaataa atcaaataat gccagcattt caaggtatat ctgaaaaagt
     9481 tgtgccaaat gcactacctg cttttgactg tccaatatta tttaattata aaccaaatgc
     9541 tgtattaata ggattcttag ttgctatgct aacatctaca gctatgattt taatatgtaa
     9601 ctactttaat gtatttaatt tgctacttat accacttgtt ataacttcat tctttgagtg
     9661 tggagcagca gcagtaatag gagaatcaca aggtggtata cgtggagcta taataggtac
     9721 agctatagct gcaatattta tggtagtttt agtaggaata tctgctgtaa tttactcagg
     9781 aactattcaa aattggatac taatatttgg aggtaatgac ctttcattat ttggtataat
     9841 cagtaggttt ataggttcac tatttggagg aatattatag atatgaaatt taaatacata
     9901 gatgagataa gaagattaat tgatataata gaaaacgaag aaaaagtcaa tatggaaaaa
     9961 gcagtacaaa tccttgttga agcaatagaa agtaaaaatg ctatattttc atttggagct
    10021 tcacatgcag gaatattaac agaagaatta ttctatagag caggtggctg tgttcttata
    10081 aatccagtat ttgcaaaatc tctaatgtta gatacagcac ctataactca cacaagtaag
    10141 atggaaagat tagaaggata tggtacagct ctagctgata aaactccaat taaaaaagga
    10201 gatgtagtta tagttcattc agtatctggt agaaatccag taagtataga gtttgctatg
    10261 aaagctaagg aaaataaggc aatagtaata tgtataacta atttaagtta ctcaaaaagt
    10321 gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag tatgcgattt agtgatagat
    10381 aatcatggtc aaaagggaga tgcctgtgta gagatagatg gattaaacca aaaagttagt
    10441 ccaacttcta ctgtaatagc aactactgtg atgaactcta ttatagctca agctactcag
    10501 gaattagtta ataaagggct aaagaatcct ccgattttct atagtgccaa tattgatggt
    10561 ggagatgagt taaataagaa gatttttgat gaatataaaa gtgtaattca ttatgaatat
    10621 taaggttaaa aaaatatgtt aatgaacttt gaagtttatc aacaaagaat attaataata
    10681 tataataaat gctatcattt aaaagctgta ggtaaaaatc tacagctttt tattattgta
    10741 gattaaaaag atgcgtaatt aaattttaaa ttggagaaaa taaattggaa ggtatcaatt
    10801 ataaatatta gaaacgttgt catagaaaaa aactatatta aaaaaaagta catagaaaaa
    10861 actgattaga caataaatac atgctttgat aatattttaa atggagtgaa aatgtgtaga
    10921 attataatga aaaaagaaaa taaaaatgaa aaaagaaaat aaaatggagg gaataaaagt
    10981 gggtatgtta aaaaaattat gttcactagc tatggtagca attatgacta caacacttat
    11041 aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag gatggcgaaa aagaaaaact
    11101 agttctttgg atgccaccag tagaaaataa tatgaaggaa gtttgggacc ctattttaga
    11161 taaatttgag gaaaaaaata actgtgaagt agatttacag ataattcctt gggataatta
    11221 ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt ccagatgtag gatatatgta
    11281 tgcagaaatg tatccacagt ttatagaaag tggagcagta gaagatttga ctaactatgc
    11341 aactaaagag gataaagagc aatctattta tataaaaaca tctgaaatga tgggtggaat
    11401 gtatggtatg ccattccaag cagcaaatcc aggggtatta tactataaca aggatatttt
    11461 agagaaatta ggagaaaaac ctcctaaaac atgggaagat tttaaaagaa tttgtaaaaa
    11521 agcaacgcaa gatactgatg gtgatggaaa gatagaccaa tggggattag ctcagggatg
    11581 gggagctaaa acttttggta acttaaactg gaactggtat ccttatttat ggcaagctgg
    11641 tggagatata tttaatgatg atttaaaatc ggttaaattt aatgataaga gtggtttaga
    11701 agcagctaag ttcttaaaag aattacaagc atatgtgcct gaagattctt tatctaaaga
    11761 tagtaatgaa atgatagaaa gtgtatttgg accagggaaa gcagcattta caataatgtt
    11821 atcttctgct gcaacaagtg tatttgatga atcattcccg gatttaaatt ggggatttgt
    11881 aactggacta gaagataaga aagcagcaac ttttggagca gtagaccatt tatctttaat
    11941 gtcatctgcg aaaaataaag aacttgcata caagttaata caacatatgt taagtgttga
    12001 atcaatgaca gaatttcata aagcaatacc aagagcacca ataacaaaag gtgaaccata
    12061 tcaaggtgat gaaagattca aagaaatggt ggaaaatgac aaaaatgtat atagaccatt
    12121 agtagtagga cctcatggag ttgaaatata tgaatattta tggaaagaac ttcaaactat
    12181 gatttcagga gataaaactc caaaacaagc gttagatgat gctgcaaaat attcaaatga
    12241 tttattagca caataaaagt ttaaagctgt aatatgttgt aaaggacttt acagattaaa
    12301 attagcattg tatttataaa tacaatgcta atatttatat tggagaggat aaaatggaaa
    12361 acttagttag agagtttgaa gaaaattcaa aaagaaaaaa aacaaaatta aatattaaac
    12421 catatatgta tatattgcca ttgggaataa ttttagtggc gttttatgtg ataccaataa
    12481 taatgtcaat atactttagt tttacaaagt ataatattat tagtccagct acttttatag
    12541 gtttagaaaa ttataaaaaa ttatttacag atgaaacatt aaaagtatct attataaata
    12601 ctattaaatt tactgttgtt gtagtacctt gccaaactat attatcattg attttggctg
    12661 tttggattac aggaaaagga aatagtaaaa tagcttcatt tgctaaaggt gctatattta
    12721 taccagtatt atcatctatg gttttgatag gtatggtatg gagggcttta ttgaatggag
    12781 aaggttctat tatatatcag gtattaggtg cctttggaat agaatcatct aaacttttag
    12841 gagatagtaa gacagcatta cctacactta tgtttatatc tatgtggaaa aatatagggt
    12901 atttcatggt aatttatatt tcagctataa tgaatttacc aaaacactgc tatgaagtag
    12961 cgaaggttga tggggctaca aaacttcaag aatttataaa aataactgta cctttattaa
    13021 aaccaactac aataatggtt gtatttttag ggtcgatatg gtcattacaa gtatttgact
    13081 taatttatac tgtgacaggt ggaggtccag gtatatctac tatgagtata gttatgcatg
    13141 catttaactt aaactttaaa aactttaatt ctggatatgc aatgactgta gcaaacgtat
    13201 tatttttgtt aatagcagta gtttcgattt tacaaaataa gcttgtaaga cgagacaatt
    13261 cagattttta gaaaggtagg tataatatga aaacagcaag aagattatta agtgatggtt
    13321 tacttttatt tatagcgtgt gcaagtttgg taccttttat atacatgttg ataatatcat
    13381 tgaagataac atataactca tatagtttgg atatatcttt ttcaacagta acccttcaaa
    13441 actatataga tatttttact aaaaaaggat ttgcacaata tttttttaat acagcaatag
    13501 tatctttttc aggtgtatta ttaaatttag tatttagtac attagcagga tattcttttg
    13561 cgaagatgga ttttaaaggt agtgataagt tatttttatt tatgataatg actttaatta
    13621 taccttccca agttacaatg ataccactat atataataat gaaacactta ggatggataa
    13681 atagttatct tgcacttatt atgccaatac caactgcatt tggcgtattt ataatgagac
    13741 aagctatttt aggagtacca aaagagttat tagaatcagc aaagatagat ggttgctcgg
    13801 attttagaat tttaatacaa atagtgttgc ctttaattaa accagcatta attacactag
    13861 caatatttac ttttatggga gcatggaacg agtttatgtg gcctcttata gctacaacta
    13921 aagatgcaat gagaacatta acagttggat tatctactct taagacattc cagataacta
    13981 attatggaca gatgatggca ggggctacaa taacattttt accgccattt attttctatt
    14041 tgattttgca gagtaaattt gttgaaggtg tgtctttgtc tggtataaaa ggttaaatag
    14101 tctaatttaa taatatataa agaaacttat ataaatatgg aggaatttaa actatggaaa
    14161 tttcatatgt actgaaagat tgcacattca aacacaataa atattcagtt atagaagctg
    14221 aaacttggaa agataaggat ttggatattg tagtagctaa aggtgaaaaa tttgcatttc
    14281 aaattatgtt aaaagcaaca gaagaattta attgtacaat tgataaaagc agtgctatat
    14341 catggaaagg cctaggaaat agagtaaggt tagcattaaa tgtgcctaat agtttagaaa
    14401 ataattttag tataaatata ttaggatatg ttcaagatga tacaaaggca tttgtgaatg
    14461 atgctatttt aagggataaa gatgtgttag tagagcaata tttgcctcaa acattttgga
    14521 ttgaaggtca agtaccagaa gattttgaag aaaataacct tgatataggt attgatatat
    14581 ttaaaagctt tggatatgaa gacgaagaaa aagtttgtac aattgatgtt cctgtaaaag
    14641 taagaaatgt tgtgctaaag cctttagatg aaagcaagtt ctttttagat ttatggcaac
    14701 atccaagttg tttagctaga atgtataaag ttgagttatg gtctgatata cattttgaaa
    14761 tagtcgataa ctatttaaaa gaattagcat ctttaggaga aaaagttgca acagttatag
    14821 tttcagatta tccatgggca gggcaaagtt gttataaagt ttataaaaat ccatcaaact
    14881 tatatgaata taacatggta agtgtatcaa aaggattgga tggaaagatt aaatgtaact
    14941 ttgagagtat ggataggtat ataggcatag ctgataagta taaaatggct aaggagatag
    15001 atttatttgg tctattaggt aattggtgtg ctggtgagtt tggaaatcct gtagaaggtt
    15061 ataaggaccc tataagagta agatattttg atgaaaagga taaagtattt aaatttataa
    15121 acaatacaaa tgatttaaaa gaatatatag gattagtatt aaatcattta atagagtgtg
    15181 gattatggga cagagtaagg attatagctg atgaaccaaa taatccagaa gtagtcaaag
    15241 agtgtataga atttataaat tctactgtag gtactcatca agttaaatat aaatcagcaa
    15301 ctcacgacca aaacttttta gacagagcta aagatgaaat tgatgatatg tcaataaatt
    15361 taaaacttac tatacaaaat tataaagata tagaaagtct taagaaaaaa atcaatgaca
    15421 agggtgggat attgacttgg tttgtatgct gttttccaga gaaaccaaat agttttttaa
    15481 gttctccttt tgtagaaaat agaataatag gatggtatac ttattacttt ggtcttgatg
    15541 gatttttaag atgggactat aatttatgga cagaagaccc ttggaaagat tcaagttaca
    15601 aattccctat ttggaaagct ggagatatgt tctttgttta tcctggaaaa gatttaaagc
    15661 cagttagaag tgtcagaatg gagaatttaa gatttggaat acaggacttt gagttattta
    15721 caatgttaga aaaagaaaag ggaagagagt atatagaagt tgagcttatg caagaattat
    15781 taaataaaaa agaaaatgct gaaattaaag gttttggtga catagaacta ggatattcac
    15841 ttgataattg gggatatgat aaagttaaaa aatatgtatt agagttgtta gacagagtat
    15901 aaaaaatata agagtgtctc aaaatgaact ttttagttca tgaggcactc tttttgtatg
    15961 aaatcaatat agtttcattg tttcaacaat gaaaaatagg attatttcta ttctaagaca
    16021 gccctttagt tttttagttg tattcaatgt tttaaaacta actataaacg ctataaagtt
    16081 tataaatgac aataattata tataacatga tgtactttaa tttgctataa tagggattga
    16141 ataatattat atggaagata acattgtttg gggggattaa tataaaaaga ataggtatta
    16201 ttataatgtt ttttttaagt atatctttta ttggatttta tcttgtaata agagataaca
    16261 gtgatacagt taagatgcaa cgagggataa atataggtaa tgcactagaa tcacctaagg
    16321 attttccgtg ggatgtaaaa atgtcaaata agttttttga tgatataaaa gatgcaggtt
    16381 ttgacacagt acgtatacca gtaaggtttt ctgattatac gtcagattct gataatttta
    16441 aaattgatga agaatttttt aaaaagattg ataaatatgt agattatgca ttggataaag
    16501 acttaatagt tgtgttggat ttacaccatt ttgaagaaat tatgaaagaa ccaagagttc
    16561 ataaagaaaa gtttttaaaa atatggcaac agatagctaa taggtatcaa aaatatgata
    16621 aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa tttatattca cagttactaa
    16681 acgaatacat tgaagaagca ataaagataa ttagaaaaac taaccctaaa agaactatta
    16741 tagtaggacc atataatttc tatcaaattg attatttgaa tgaattaaat attcctaaag
    16801 attctaatat agtagtttcc tttcactatt acgagccaaa tgattttgca tttcaaggaa
    16861 atatctatca taagggattt gagcacttaa gcaatattac ttgggaagga acaaatgaac
    16921 aaatggatta tttaaaaaaa aggtttgata ctgtggaaaa ttgggcaaat aaaaataagg
    16981 taaaaatctt cttgggtgaa tttggagtaa caaaagaagc accagaaact tcaaggagag
    17041 cttgggtaaa agctgtaaga gaagaagctg aaaaacgaaa cttttcatgg gcttattggg
    17101 agcttgcttc tggatttggt atatacaatc aaattgaagg gacttgggat agagaaatat
    17161 taagtgcatt aatagaaaag aggtgatttt atgaagaaaa attcaaaaaa gatacttata
    17221 actttaacta ttattactaa tatagtatat atattgtgga gaatttttta tacagtacct
    17281 aaagaagaag ggatgtttgc cctaatctgt gccataatac ttctttttgt agagataatg
    17341 ggtatgatgg aaatgtttgt gcattattat ggtatgtcaa atattgagta ccctgaaaaa
    17401 ccaatcataa gtgaagaact ttatccacat gtagatgtat tcattgcaac atataatgaa
    17461 tctgttgatt tggtgagaaa aactgtaaat gggtgtattc atatgcagta tccagataag
    17521 aaaaaggtac atatatacat atgtgatgat ggaaatcgtg aagaaatgtg tattctagca
    17581 gaaaaaatgg gtgtaaatta cataactaga actgaaagag aaggagcaaa agctggaaat
    17641 ttaaataatg caatgcaaca tacaaattcc cctttgattg ctacatttga tgcagatatg
    17701 ataccaatgc atgacttttt aattgctaca gtaccatact ttttgaaaaa tgagcaagct
    17761 aaaaaagatg gagaaaaaga ggagtatgaa aaggttggtt ttgtgcaaac tccacagagt
    17821 ttttataatc cagacttgtt tcaatttaat ctgcactctg aaggaaggat tccaaatgag
    17881 caagattatt tttacagaga cattcaattg gctagaaata ggacaaattc tgtaatttat
    17941 ggtggtagta atacagttat ttcaagggaa gcacttgaag aagttgatgg attttatact
    18001 tattctatta cagaagattt tgcaacaggt atcttaattc aaagtaaggg gtatagatgt
    18061 tatgctattc cagaagtaca tgcatcaggt ttatctccga cagatttaaa aagtcttatt
    18121 aagcaacgtg aaagatgggc acgtggatgt atacaaactg gtagacgtct aaatattttg
    18181 tttaggagag gtttaggttt ttggcagaaa ataagttata tttcttcaat tacttattgg
    18241 tatgcaagta taaaacgctt tgtatatata atggcaccta ttttattttc tgtgtttaat
    18301 gtgattgtag taaaatgtac gttacttcaa gtattggtat tttggttgcc aatgtatatt
    18361 ttaagtagtt tatctcttaa aatattttct caaaatataa gaaatactag atggaccaat
    18421 atatatgaaa ctataatgtt tcagtcttta atgccagcag taatacttga aacgtttgca
    18481 atatctaaaa ataaattttc agtaacaaat aagagtaaat tagaagaaaa tagaatgtat
    18541 aagtttttac agggaatacc ttattttatt tacatggtct tatctatcat aggaatttta
    18601 aaaatgtttg tagcaatatt taaaatgagt tcaatgacat attcagttgt attattttgg
    18661 ttgattggaa acctttttaa ccttgttatg gctacattat ttatatcagg gagacagcag
    18721 ttaagaaagt ctgagcgtta tattgctgaa attgatttta agttaaagca aaattcttat
    18781 gttctttctt caaaaacaat tgatatatca gaaaatgggt ttgcattttt gctagaaaat
    18841 ccagaatata tttccccaga agaagagttt gaggtagagt ttagagaaaa atctggaaat
    18901 gaaatgtata tagctaatat gaaagcaaag attgttaatg ttgtggaagt aaattcaaag
    18961 tggaaatatg cagcttatat tacacatatt gaggatagtg aaatagataa ttggatgtgt
    19021 attgttcacg atagaatacc tactttacca atgacaatat ctaatcaact aggtttcttt
    19081 gatgatttgc aaattaatgt aaaaaaacgt attgaaaaaa ctagaacatt atcacgaaga
    19141 agtccaagaa tcaatatgaa ttttcagatg gatataaaaa atacaggtaa attaagaata
    19201 gtgaatttta attatcaata tgtattatta aattttgaga ataaaagtat ttatcctaaa
    19261 gaaatagcat tggaaattaa tgaaaatatt gtgttagaat gtgatttgtg tgagggtaaa
    19321 attgatgaaa gaggtatctt atataaagta aataacattg acagcataat gcaaaatttc
    19381 tttttaagag atgaaatgat ggattggata ttacaaaata agacaattct tgtttcaaag
    19441 cctagtgaaa agaaggaaaa aagtatagat gagtttgaac ccatggaata tatttaggag
    19501 agatgaaatg aagactttaa aagtgttttt ttgttctaca aaatatctta ttttggtgtt
    19561 tatttttttt ataaatacta cttttataag ttcagcaaca agtgataaaa ttttagattt
    19621 atcctttaaa aagatagaga ctgacttaag ttctaagatt acatatgagg atacaggtgt
    19681 aagaatagaa acggatagtt ctaaaagcga taaggaacgt tatttatata tttaccaaaa
    19741 tataaaagaa aattggagca tgtataataa tttttatatt gaaattcaaa ataaaaataa
    19801 aagttctcag agaataaatt taagcataca aagtaaaaat atgcttgaat ttagattaaa
    19861 agaaggtagt gaagtatttt tagaaggtaa aaatataata tattcagata aaataaaaga
    19921 aggatgcata gaagtaccag gtgaatttga aggcaaaatt tatgtgaact ttaatagcct
    19981 tataaatgag gaaagtaatg ttgtgctgga ctcaaatatg ctttcaaaca ttgttagttg
    20041 gggaattacg tttattccat ctgatgaaga acataatatt gttattataa aaaagatatc
    20101 tttattgtct gaagagaaat taagattttt aaataatatt aaaataattg gcgatgaaga
    20161 agtacaaata ccagtattgg gtcaaagtat aagtcagtat gaagtattag gtcttaaaag
    20221 tgattctaaa attaagtact ctcttatggg aaaacaagat aatgtgagca tatctcaaaa
    20281 aggtaaatta acattaagta ataaagccaa acctggacaa attattttac aagttaatgt
    20341 tgatgataag tttaaaattg gaaagaaaat aacattaact gagtcttgga gtgttaataa
    20401 aaaagataag gatggtgttc catatacatt agtatatcct gagcaatcac caacagtaca
    20461 agatatgaaa aaaataaact ttatgaataa catcataacg tttgttcgaa ttttatttgt
    20521 atctctagta ataatatgct ttggaatata tttatattgg aaaaaatgtt caaaaactaa
    20581 gtaaagagag gaggttagat tgtgttattt tcaagtttaa tttttttatt ttatttttta
    20641 cctataactt tggttttata ttatgtattt aggtttaacc gaacgataca gaacatgata
    20701 ttgcttgctg ttagtttatt tttttatgct tggggagagc caaaatttgt tgttatcatg
    20761 atagtttcca taataatgaa ctatatattt ggtttactgg tagatagata tagggaaagt
    20821 aaaataaaag taaaagtttt tttagttttg atgtgtgcat acaatatagg tgttttattt
    20881 atatttaaat atctagcttt tgcgctaaga aatattagtg aacttattag tactgaattg
    20941 actataccta gtattgtttt acccatagga atttcatttt ttacatttca gggaatgtct
    21001 tatgttatag atgtatatcg tagacatgga gaagtccaaa agaacccttt ttatgtggga
    21061 ttgtatatag catttttccc acaattaata gcaggtccta tagttagata tgagagtgtg
    21121 gcagaacaaa ttctcaatag aaaagaaact tggaataaat tttctattgg tacttgtagg
    21181 tttataacag gtctaggtaa aaaagtatta atttcaaata acatggctat agtagcagac
    21241 tatatttaca ctatgaatag tcaaggagaa atagctgcat cacttgcatg gcttggttca
    21301 atagcctata ctctgcaaat attctttgat ttttctgctt attcagatat ggctataggc
    21361 ttaggtctta tgtttggttt taaatttgaa gagaacttta attatccata tatatcaaaa
    21421 tcaattagtg agttttggag aagatggcac atttctttgg gaatgtggtt taagagctat
    21481 atatactttc cgcttggagg ttctagagta gcaaataaag atattatgat tagaaatatg
    21541 tttatagtgt ggttattcac tggaatatgg catggagcag aatggacatt tgtaatttgg
    21601 gggattttaa actttgtttt cctaattata gagcgattta taatgtttga aaagattgaa
    21661 aatcacaatt ttataaaaca tatttatgct ttattagtag ttaattttgg atgggtattg
    21721 tttaggtcac ctaatttaaa agaagcatat aattatttta aagctatgtt tggaggcaat
    21781 ggagctatat ggagtgatta tacatatatg tttttaaaag aatactgggt tttctttata
    21841 tttgcattta tttttagtgt acctattgca aagaaaatca ataagtttgt tgttgaacaa
    21901 gctaaatata gtatgatatt taacgtattt tatccaatct cagctatttt attgtttttt
    21961 atttctgtga catatttagt aactggaagc tataatccat ttatttattt taatttttag
    22021 ttgggaggaa aagtatgaat agatttttta aagtaagatt aataactgca attatgttct
    22081 taattatttt attcgctttc tctatcttta atgtttgtta ttctggaaaa gatacgatac
    22141 aggaaataaa aaattcaata gctgaaaaaa gtagtttaaa agatacaata tcatctattg
    22201 ataatacagt gaatgacaaa gtaatataca aaaatacgtt cattgaaact tatggctatt
    22261 tacaaaaatt aatgggtaaa aatgagtttt caaaatttag tgttgtaaaa gatacttctg
    22321 gaaagatgca ttatacatat tttgctaaag ctccaaatcc agttggtgtg atagcagata
    22381 gggtaagaaa attttctgat gaaatggaaa agcaaaacac taaattggtt tacctaatga
    22441 ctccagataa gtatataaaa ggagtgacaa aatttcccaa aggaatacct tattcttata
    22501 gtaatgagac agcagataat tttttaaatc aactaaaaaa gtataatgtg gactatattg
    22561 attttagaga aaatatttta aagagtggta ttccaaaaga tgatttattt tttaaaacag
    22621 accatcactg gaaagtagaa acttcttttt gggcatttgg agaattagtt gagcaactta
    22681 ataaaaaata caatatgaat ttagatgaaa atcattatta tagagataag gacaattata
    22741 attcaattgt atatcctaag tcatttcttg gttctatggg aagaaagact ggtatcttat
    22801 atggtggtat agatgatttt acattaattt atcctaaatt taagactaat tatacttatt
    22861 atacagattc taaatctcaa aaatttgaat taaaaggaag gtttgaagag agtttaatat
    22921 tatcatatcc ttttaatgca gaccttaatt taatggatgg tcaatcagat aagtatttta
    22981 catatttatt ggggaacaga ccattagtta aaataaaaaa tatagaaaat ccagatggat
    23041 taaaagtgct gtttgttaaa gattctctaa ttgtaccaac agcagcattt ttttcctctg
    23101 tttgttcatc tatagatatg atagacccta gatattatga tggagatata ctagaatatg
    23161 caaaatccca tgactatgac tttgttttta tatctgtata tccccaaaat cttacaaaag
    23221 aattttttcc gttttgtgag taaatttcta aaaaagtttt ctgataaaat aaatggctaa
    23281 ctggtaaaaa gttagccatt tattttgagt gttattctaa gaaatatttt ttttactaaa
    23341 tacttcttta tctatttttc caagtgattt tcctacagta attgatgata ccatagcacc
    23401 attaacattt atcatagtac gtcccatatc tagtataggg tctattgcaa gtataccacc
    23461 aactaatgga aaataagaac caagaccaac tccagagata acaactgaaa cggccattgt
    23521 agcagttcct ggaagaccag ctattccaaa cgaactaatt gttataacaa ttaacaacat
    23581 aacataaaag ctcatatcca tgtttactcc agccatattg gcaagtgtta cagccattaa
    23641 agcaggatat atacctgcac aaccattcat acccatattt gcacctaaac ttgctgtaaa
    23701 gcttgataca ccctcatcaa ctccgtgatt ttgtactaga ctttctatcg ttacaggcaa
    23761 tgttcctaaa ctagaacgtg aagtaaatgc cagaagtaaa gcttcagttg aattttttat
    23821 ataagtaatg ggatttagac catttaaagt tataaaaatt aaatgtatga taaataatat
    23881 tgcaactgat atataaagag ctaatataaa ctgtaaaaca gatactattg aactaatacc
    23941 ttgtgaagtt attgtatttg caagcagagc aaccacagca taaggcataa atttaataac
    24001 agtcatagac acacttacaa ttattttgta agatgcttct ataaaatcta taaatggttt
    24061 tattgtctca aaatgttttt tacttaatct ctttattgag gtagctataa atgtagcaaa
    24121 tattattgta gctacaatat ttccatttgc catagccatt acaggattag atggtagtaa
    24181 tcctcttaaa gtatccacaa gagagtttat ttctttaagt tcttgatttt caactaaagt
    24241 aggcatatta gctcctacac ctaacttcat tatatttgca acgataattc caacaattgc
    24301 agctatagca gtagtagcaa gaagcatacc taaagattta aatgtcagag ttccaagatt
    24361 gttatctttc atatttataa ttacttttat aatggataag aaaactaaag gtactactaa
    24421 catttttaat aagtccataa atccagaacc aaataggcta taccacttac ttacttcttg
    24481 tagccaaatg acttgagttg tatcattagg gaaaccagca aaagcttgta ttatgattcc
    24541 caatatgagt cctagtactg tagcatatat tgttctcaat gaaaatttta tttttttctt
    24601 ttcaaattgt ctaacaataa agaacgtacc tagaagtaat attatgaaaa aaattgtttt
    24661 aaaatctgta atcataagaa attgtgataa aaatgaactt tccaatattt ttctcctttt
    24721 atttaaaaat aaatttttct aagtttaaat gatatcaata tattaaaaaa atgtcaatat
    24781 tatatcacaa tttacttaaa tagagtaaaa caagttgatt ttgaaaaatg tgattaagta
    24841 cagaaaaata gggtataata aatgtttaaa gaatttgaat tgtggtaaga aaataaaaaa
    24901 tagcttttat aagatttgat ttcaaattaa aatgtgttaa ataaataact aaaaattatt
    24961 ataaaaaatg ttttcataga atatatatca aatgaaaagc ttaaaataag tgaagaaatt
    25021 aactagacat caaaggagag ataaaatgag agtaataata attggtggtg ttgcagctgg
    25081 tatgagtgct gcagcaaaat taaaaagaat aaaacctgag tatgaagttg ttgtttatga
    25141 aaaaactgaa atagtatctt ttggtgcttg tggattacca tactttgttg gtggattttt
    25201 taatgatgct gatgaattat tagcaagaac accagagaaa cttagagaag ctggaattga
    25261 tttaaatata tttagagaag tcgttgaggt tgatagtgaa tctaagaaaa taaaagtaaa
    25321 aaacataaaa acagatgaaa tatatgaaga ttactatgat aaattgatga tagcaactgg
    25381 agcaagaagt attatgcctt ctataaaaaa cataaaattg aagaatgttt ctacattaaa
    25441 aagtttatat gatggagaat atctaaaaaa acttttaagt aatgaagata ataaaagagt
    25501 gacaataata ggagctggtt ttatagggtt agaagcagtt gaggcatgta agaaattagg
    25561 taaagatgta gatgtaattc agttagaaga tagaatattg ccacaagtat ttgataagga
    25621 gatgacggat gtattagaag aagaaataaa tagacataat gttaatttgc acttagatga
    25681 aatggttgta gaactttgtg gagaagataa agtagaaaaa gttataacaa acaaagggga
    25741 aatagataca gatgtagtaa taattgctac tggcgtgaga cctaataccg agtttttgtc
    25801 aaatactaat ataaaaatgc taaaaaatgg agctatagta gttgatgagt atggaagaac
    25861 ttctgtagaa gatatatact ctgctggaga ttgtgcaact ataaagaata tagtaagtaa
    25921 tgaaaatgta tatgttcctt tagctactgg agctaataaa ttgggtagga tagtaggaga
    25981 aaacctagca ggaagagaag tatcttatca aggttcacta tcttcaagct gtataaaaat
    26041 tatggatatg gaggcagcat caactggaat tacagaaagg caagctaaag atttaggcat
    26101 aaatgtcaaa tctaaattta tttctgatta taatcaaact cattattatc caggaagaaa
    26161 taaaatatat gtaaagctta tttatgatgc agatacaaaa gttattttag gcggacaagt
    26221 ggctgggttt aaggatgctg tccaaagagc aaatgtattg gcagctgcta tctttggaaa
    26281 aatgaccaca agtcaacttg gaatgttaga tttatgttat gctccaccat ttgcaagaac
    26341 atgggatgtt ttaaatgtag ctggaaatat atctaaataa tatttagatt atagctaata
    26401 tagttgacaa gcaattaagg gaatgaattt acctaaacta tttttatcat ggtaaaattc
    26461 attcctaaat ttttattcca aatttttatt ccaaattttt agaaataagg attccattaa
    26521 attattcatc aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa
    26581 aaacattagt agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatgag
    26641 tcagtaaaag ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca
    26701 tatgtttgtt tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata
    26761 taaataaatc actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg
    26821 taagacttat cttaactccc tttcttaatt ctcctaagac catatctgga gtgtattgtt
    26881 ttgaatctaa aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt
    26941 cgtttttagt tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc
    27001 ctaaacactc tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt
    27061 tattatctaa agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat
    27121 attctactaa atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag
    27181 ttaaatcact tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata
    27241 tatggtcacc atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt
    27301 ttagcttcat agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat
    27361 agtttataaa tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa
    27421 gtaaagttaa atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt
    27481 tttattatga tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt
    27541 gagtcaagaa atattgtcaa ataaagtttt tatataacaa tgtataaaaa tttggaggac
    27601 aaaaagtgaa aattatattt agaagtattt aaaggaggaa aatttaatga aagtattaaa
    27661 gaaaattact attttgttat tgattacatt aactttagtt ggttgtagta acggagaaga
    27721 gaaaaaagtg gttgaaggtt ctaagaaaac tcaaaattct aaaaaagatg aacagcctat
    27781 aatagtgcat aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa
    27841 aagagttgat gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa
    27901 ttatggagag aaagtaaagt attatgatgg aagaactaag gaatcaaaag gtatttatga
    27961 tagtgtattt gatgttgata ttggaaatag agatttacat caatgtgcag atgcaattat
    28021 gcttatgaga gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt
    28081 tgttgatggt tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa
    28141 agatagtggt tcaagctggt ataaagcaac tgaatattca acatcttatg agagtttcag
    28201 aaagtttatg gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga
    28261 gcctgtaaaa gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg
    28321 acatgcaagt ataattatgg atatggcaga aaatgaaaag actggtgaaa aggtatttat
    28381 gatagcccaa agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc
    28441 aaatttgagt ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac
    28501 ttttaaaaga gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa
    28561 tatagatgtg aaaaaatgta attagattaa aactaattgg atatggacaa aattaaattg
    28621 atagataaat attaaaaatt acactaatta atcttactat agtttaatat ttatctaaaa
    28681 agttttatgt aaaatataaa ttttatatta aagctaaaac taatttgagg aggctaattt
    28741 atattgaaga ttagaaggta tttaattttg gtaattataa ttctaattac aacaagtcag
    28801 agttttattt cttatgggct agagcgattt gaagaaataa ccatttttca cactaatgat
    28861 atacatggaa gatatgctga aggtgatgac catatacaaa taggaaatct agccacattg
    28921 aaaaaagaaa ctccaaactc aattttagta gatgcaggag attgtttgca tggacttcca
    28981 atagtaaata tggatagagg gaaaagtgca attgaattaa ttaaatgtgc tggatatgac
    29041 tatattactc ctggaaatca tgactttaat tatggaaaag atagactgtt tgagttatct
    29101 aaaagtgcta atttaggaga gaatacttta aaatttttat catcaaatat ttttgaaaat
    29161 ggaaaaaaag tttttgatag taatgatata aaagaaatag atggaataaa aataggattt
    29221 tttggactct caacccagga aacaaagagc aaaactggat ttaaaaatgt ggaaggatta
    29281 gagtttaaaa atcctataga aagtgcaaca gaacaagtaa atgaattaaa aggaaagggt
    29341 gccaatgtaa tagttgcaat atgtcatatt ggtacaaata gtttaagtaa gccaaatagt
    29401 atagatatag caaatgaggt tcaaggaatt gatttgatta ttgatggaca cagccacact
    29461 aagtttgaaa atggtaaaca agtgggtgat acattgattg taagtgcagg acaatattta
    29521 gaaaatattg gcgagataaa actaactttg gatactaatg atgtagaaaa tataaaaata
    29581 gaaaataaat cagttagatt aattggaaaa gaagaagcat taaagtataa gcttgactcc
    29641 gctgtaagta aaaaggttga tgaaataaaa gaacaacaag aaaaaatatt agataaagtt
    29701 ataggggtaa ctacaaatac tctagatggt tcatatgaaa atgtaaggac aaaagaaact
    29761 aatttgggta atttaataag tgacatttta ctagataaaa ctaaggctga tatttcttta
    29821 tttaatggag ggaatattcg agatacgata gaaaaaggca atataacaag aagagatatt
    29881 gtagatgtat ttccttttag caatactata gttaccaaag aacttacagg agcacagatt
    29941 aaagatgtat tggagcatgg agtaaaactt tatccagaaa aaagttcagc ctttttgcaa
    30001 gtgggaggaa tttcatatta ctttaatcct aaacaaaaag aaggcgaaaa aataacaaat
    30061 attcaaaaag aaggaaagcc attagattta aataaaaagt atgtagttgc aacgaatgat
    30121 tatatagcat caggtggaga tgaatttcct tgtttttcaa aagagccagt actaaaggaa
    30181 tttggaaatt tagagagtgc agtcattgaa tatatagagt acaaaaaaga aatatcaaaa
    30241 aatgtagatg gaagaattgg tattaaatct aaagatgagt taataagcaa gaatcatgag
    30301 agtgatttga tattaagtga aaatgaaaaa aatatcaatg ataatgaaga gaatctatct
    30361 aataatataa aaagctctga tagtgataaa aataaaagct tagataaaac aagtgctaga
    30421 gaaagttcta atctaggaat atctaaaaga gaagctaaag aaaaatctcc taaaacagga
    30481 gatttaggtt tttcaaatag tataattata tttatagttt caagtacatt gatatgttta
    30541 ttgaatttta atcaaaaaga gttaaaggat aaaaagtcaa aataaaaatt aatgagcaaa
    30601 aattagtaga tagtaattta attttagata atagtaaaca gcaaaggaaa ttagttatat
    30661 aaatactaat ttcctttgtt ttatgtttta acatgattac ttttaaataa aattgtattt
    30721 tatgaaaaat aattacacta aatggcatag tattatgggg atttttagtt tttattcaag
    30781 taaatgaaat catattaaca acttctgata tatttacaaa ttgataaaaa aatgatttta
    30841 ttagtatgat ataatttagc tagtatatat gatgatggag gatataggga tgaataatat
    30901 attacttttg gaagatgaca aatctttaaa tagaggaatt agttttaaat taaaaaaaga
    30961 aggatacaat gtttttagtg catttggtat agaagaagca aaaaatattt tcgcaaaaga
    31021 agagatatgc ttaattattt ccgatatagg tctacctgat ggtagtggtt ttgacttttg
    31081 tgaggagatt agaaagaaaa gtgatgttta cataataatg ttgacagctc ttgatgaaga
    31141 ggtggatatt gtcacaggat atgatttagg tgcagatgat tatatcacaa agccttttag
    31201 cttgatggta ttaatatcaa aagtgaatgc gcttatgaaa agagtaaata cagtaaaaaa
    31261 ttatactcta ttagtttgtg atgatttatt cttttattat atagaaaata aattaattat
    31321 aagaacagat gataaggaag aagagataat tttaagtaag acagagacaa aactattaaa
    31381 atatttgatg gaaaattcta tgcagacatt gactaaagag caactgttag aatctttatg
    31441 ggatagtagt ggtaattttg tagatgataa tacgattgca gtaaatataa gaagacttag
    31501 acaaaaggtt gaaaaaaatc catctgaacc taaatatata aaaacagtaa gaggggttgg
    31561 atatatgtgg ggagaaagga gtataaaaaa atgctaaaaa atatgaacct caaagatggt
    31621 ttaagtgtga aagatgatta tataatgaga agagttattg tatattctgt tttatctgta
    31681 ctaatagtaa cccttgtatt tatacttttt gaatatagca gattacataa attgtatgaa
    31741 catcaaataa aaaatactca aaatattgct ggaatattga ttgaaaaata tcctaatgat
    31801 gaaatagata tagtaaattc tatttataat tctgattata gtaaggtgtc tactggagaa
    31861 aaggcattta agaaatttgg ctatggattg gaaaataaaa tgtcaaatga taaaaatttt
    31921 agcttatact taaaatcttt ttttaaggaa tcatttttta tttttttaat gatgtttata
    31981 gtagttatag gtataatata ttattttata agatacataa ataaaagatt gagtaaaatt
    32041 tattttatag tagataagat gtcacaagaa aactacttga aggacactac tgagtcagaa
    32101 gaaactggtt atctagtaga atataatgaa tactttaaag aaggcatatt tagtaagata
    32161 aataactgtc tatatgagct taatagaagt ttaaagataa agtttattaa attagaaaaa
    32221 gaaaaagaaa gtgtaaaatc attagtaaca gatatttccc atcaattaaa aactccttta
    32281 gcatccttaa aattatacaa tacattactt atagaagaag aattagaaga agatgaagcc
    32341 aacgagttct tattaaccaa taaggcttct ataaataaat tagaaaattt aataaattct
    32401 ttagtgaaca tatcaaggtt agaaattagc atgataaaca taaaaaaaga agataatgat
    32461 ataaaaaata ctattctaaa tgcagtaaaa agtgttaccc ctaaggctaa gagcaaaagt
    32521 ataacattaa atttaaatga atttaacagt agaattatac cacatgataa aaagtggact
    32581 gaggagagta tttttaatgt attagacaat gctgtaaagt atacacaggt aaatggagaa
    32641 ataaatatat ctgtagaaga aactgctaat ttctttaaaa ttataataga ggataatggc
    32701 atagggattg aaaaaaatga gtttaataat atatttaaac gtttttatag aggttctgct
    32761 gatgaaattg aatctgtaga aggtagtggg gtaggattgt atttaagtag aaagatttta
    32821 gaagaacaag gtggaaatat tatagtttct tcaaaagtag gaatagggag caagttttct
    32881 ttgtttctta caactatgta atattaaatg aaaggaaaat gtaagcctct acatttatta
    32941 tataagtata aacagctcca gaaataaaaa attaataaaa tatggagtaa tataacttag
    33001 aatgagagga taattttatg aaaatactat atactgaaaa tttatcaaag cattatggaa
    33061 aaggagaaag cttagtaaga gctttggaca atgttgactt agaaataaat gaaggtgaat
    33121 ttgttgcaat aataggaaaa tcaggttctg gaaaaagtac acttttgcat atgattggag
    33181 gtcttgatat acctacatct gggaaggtgt atatagataa taaaaatata tttacattaa
    33241 aagaagaaga acttgctgta tttagaagaa gaaaaatagg gtttatattt caaagctata
    33301 atttaattcc atctcttaat gtatgggaaa atgtagtgtt gccaatcggt ttagatggta
    33361 gagaagtaga tgaatctttt ataaaagaac ttttaaaatc attaggactt gaaaataaac
    33421 acgacgtttt accaaataca ttatcaggtg gtcaacaaca aagggtggca atagcaagag
    33481 cattagctac aagaccagct ataatacttg ctgatgaacc aactggaaac ctagattcta
    33541 aaacgtctga tgaggttatg agtatattaa aatctatgtc taagaagtac agtcaaactc
    33601 tagttatgat aactcatgat gacagtatag cccaaatggc tgatagagtt atattcatag
    33661 aagatggcag ggtgtcaaag gttggtgata aaaatgatta atactactaa aatagcaaaa
    33721 agtaatttaa agcaaaataa atcaaaaagt attctaataa taattactat aatactttca
    33781 accttaatgc tttcttctat tggaatttat attgttgatg caggagcata tcaaaaagaa
    33841 aacactataa agtattcagg taattatcaa ggtatattag caaatgtaga tgaaaaacag
    33901 gctgatatat tgagtaatca tgcagatgtt gaattaactg gtgaaatgaa tggagtaggt
    33961 gttgaaaaac tggaagatga ttcaaatatt tctctagcat acatgaatga agatgctttg
    34021 aaattaaata gttttgaatt tatcaaagga aaattgccat caaaagagaa tgaaattgtg
    34081 ttagatagtg gagctttaaa ggcacttggt tataaaaata aagatttagg tgaaaagata
    34141 aaaatatctt atgatgacta taaaaatgat aaaaaaattg aaaaagagtt cattataagt
    34201 ggaatcttaa aaactagcga aatatctgaa gcaggtaaat attattatgc tattatatca
    34261 gaatcatata tgagaaatac tagagatatg tcacaagaag attttaatat ttatgtgaaa
    34321 tttaatgata aaagtaattt atcaatagag caagcaaaag aaaaattaga taaaattgct
    34381 aatgatatag gactggatac tattaatact gctgtaaatg aaaattatat aaatgcttta
    34441 aaaccagata tggaaacgat tatgggtgga gtttttgtag ggttagtaat tgtattatct
    34501 agtattttag taatatacaa tatattttat atttcaatag ttacaaaggt tcaagaattt
    34561 ggaaaactta gagcaattgg tgcaactaag aaacaaataa aaaatatagt gtttaaagaa
    34621 gggttcatat tagccggaat agcaatacca ataggaataa ttttaggcta tgtacttgca
    34681 aatattataa taaaatcatt tatggatata gatgtaaaat catcacgatt accagttata
    34741 ttatcggttg tggtaataag ctttatatct gttgttttat cacttttaaa accaatgaaa
    34801 gtagcatcaa aagtatccat tgttgacgca gtaagatata caggaaataa aataagtaat
    34861 aagagtaaga gaaaaggata taaaaatata aatctaaaga gattatcaca tgccaattta
    34921 gaaagaaata aaaaacgtac ttacatgaca ttggcatcac ttatacttag tggaactata
    34981 tttataacag tatcaactgc cttagaaagt tttgatgctg agaaaatggc aagagaacac
    35041 tttccttatg atatagaagt tagactatct ggttatgaaa tgaacagtga taaaaatcct
    35101 aaagataatt tgaatatttt acaaatggat aatcctctaa gtaaagattt ttttaatcaa
    35161 ataaagaaca tggaaggaat taaaagaata gaatctgcaa gaagtgtaaa aattggtatg
    35221 gaggactatg atgttgaatt taagtatgac cttttacaaa gtataaatga aaatgatgta
    35281 aaatcattaa acaagaatat aatagatgga aaaataaatc ttgaaagact tcaaactgga
    35341 gatgaaatag taattactca tgttgataca gcaaaagaaa tgggagtaaa agcaggagat
    35401 aaaataagat taactttata tgatggagat aaaaagataa aaaaagaatt taaagttcaa
    35461 gcaatagcta tgggggttcc aagttttggt atagggaaag attttataga tagaactttg
    35521 aaatacgatt caactagtag cttgggaata tatacagaag aaggaaagta tcaagaaatc
    35581 aaagatagta tcaagaagat tgctaaaaat aatggatttt tagagactga ctttatagat
    35641 agtagaattg agtcaaataa ggctactatt tcatttataa agataatggg atatacgcta
    35701 acaggcataa taggtgtgat tggatttatg aatcttgtca acacaatgat aactagcata
    35761 gttactagaa aaaaagaact tggtatgctt caagctattg gtcttactaa taaacaatta
    35821 gtgaaaatgc ttaattcaga ggcaatgtat tacacttctg gaatgatgat aggaagtatt
    35881 ctatttggag gtatattagg gtatattgct gttatatttc taaaaaagac tggattatct
    35941 tatgcaacat attctttgcc aatagtacca atcttgttga tgattgtgtg tattcttata
    36001 gctcaattta taacaactta tttgatagga cgaagtttta ataaagaatc tcttattgat
    36061 agagtaagat acagtgaata aaatgcaaat atttatggtt agtttaacta gcaaaaaaca
    36121 tataaaaaaa tatagttaaa gaaattcata attaaatagg ttaaagcata gtacagagca
    36181 aacaatttaa acagatgttt gctctgtatt ttattatgta atcaataata tatctaatat
    36241 actgatatta ttcatgttag aaaatgtacc ggtacaattc taaaaaagta taaaattgta
    36301 ccatataaaa tgaaaatttt gtataataat cgtatagaaa ataaatagga gctgatatat
    36361 atgaataaga agattaatta tgcaccagga ccaactgaaa ctagggaaaa tgtaagatta
    36421 gcaagagcag aaaaaacaac aaatccagat atagatattg attttgttga attttataaa
    36481 aagacatgcg agaagtttgg taatataatt ggaacaaaaa acgatgttta catattgagt
    36541 ggtgaaggaa tattaggtct tgaagcagca tgtgcatcac ttacagaaaa aggagataga
    36601 gtacttgtaa ttgataatgg tatatatgga gaaggtttta aagattttgt aacaatgtat
    36661 ggtggggagt atgtgctttt ttcaagtgaa tatactaaaa gtatagatat tgatgaattg
    36721 aaagcatttt tagatgagga tagtaatttt aaatatgcaa cagtagtgca ttgtgatact
    36781 ccaactggcg tattaaatga tgtaagcaaa atatgtccat tacttaaaaa atatggaata
    36841 ttaactgtag ttgattctgt agcaggtatg gtaggagaaa gattaagtgt agatgaatct
    36901 aaaatagata taatattagg tggttcacaa aaagctattt ctgcaccagc aggacttact
    36961 atagtcggaa taagtcaaga tgcaaaaaat tgtataaaaa atagaaaaac tgatgtaata
    37021 ggtttttatt gtaatttaaa tatatgggaa ggctattatg aaaaaaaata cttcccttat
    37081 acaatgccaa taagtgacat aatggggctt gataaagcac ttgataatat attagaagaa
    37141 ggaatagaaa atgtattaaa tagacatgaa aagatagctt atagtgttag aaaatctatt
    37201 gaggaatatg gtctagaact atttttagaa gaaggatatt ctaatacagt cacagctata
    37261 aaagttcctg aaagtatagg agctttagaa cttactgatt atatgcttaa aaattataat
    37321 acacttgttg caacatcact aaatcaatat atggatacta ttttaagaat aggtcatatg
    37381 ggagagaatg caaatctaaa taaaatagaa catgtactaa atgttttgga taaaagttta
    37441 agtgcactag gattcaaagg aaatggaaat ttgttaaatt tatttaataa gtattatttt
    37501 taatagatag ggggatatag tatggaagtt acagctaagc aaaaatttac aacgaaagac
    37561 atggtggaaa cctcactatt aatagcatta gtatttattg caactaaatt tataaacata
    37621 agattaccaa tatctataaa tggtgggctt gtgcatttag gaactgcaat gttgttcata
    37681 tcagcaataa tatttggagg taaaaaaggt gctttatcag gggctattgg tatgtcactt
    37741 tttgatttga tatctgggtg gactttatgg gcaccgttta cctttatagt tagaggagtt
    37801 atgggttatt tacttggaaa gattgcatgg gcaaatggta ataatgggaa taatttttta
    37861 atcaatgtaa taggaatatg tgtctcatca atttggatgc tatttggata ttatgtaaca
    37921 gaagttatac tttatggtaa ttttatagta cctcttactt caataccagg taatttaatg
    37981 caagtattga taggtcttat aatagcgctt cctatttcta aggtattgaa aaactgtata
    38041 aaataaatat aaagtgaact aaaatttatg atagcataaa ttatactgta taatattagg
    38101 atggagtaca taattaaaaa attttagtat agaggttagt tcacaatgtt atcagttaca
    38161 agatattcaa tcaaaaatgt tcgtttaaag ccgtgggtaa agtttatttg gtattttgag
    38221 acaaaatcaa atatactgtt aaataataag ttacttccga cagacagtat agatattatt
    38281 ctcaatcttt ctgatgttat ggaatataaa attgaaaatc aagactatac tgctagtaat
    38341 atgcatttta atggaataag ggataaacat ggatttatta ttcaacatgg aaatatacgt
    38401 gttataggga tttcttttta tccatttggt ttatatccat ttttaaagat accaatttca
    38461 gagtttaaca gacaaattgt tgacttagaa gtagtatcac agctatttgc aaaaaaattg
    38521 gaagagtcat taagtccaac acaatcagta gaaaaaattg ttttatattt agaagaagta
    38581 ttattatcca tattagaaga agatttaatt tcaaataaat atgtaaagct tctaaattca
    38641 tttatatatg ttaatagata tagcaatata aaggcatttt gtgatgatac aaatataaat
    38701 attaaaacat tggaaagaat ctgtttaaaa tatacagggt atacacctaa gattttaaag
    38761 agaatttaca gatttaaaat ggcaagtaat caacttattt ataattataa agatgatgaa
    38821 ctttttgatt ttatttatga aaatgagtac tatgaccaag cacactttat aaaagaattt
    38881 aaaaaatttt cagggacgtc accaattaaa tttattggtg agaataagac gattaaagaa
    38941 aatataaagt acagctattt ataattatac tgtcgatttt ttacaatacc tccaatttat
    39001 tttttgatat gatataggaa aataaattgg aggttttaca tatgaaaatt attacagaaa
    39061 tagttgagtt taatgtggaa gaaaatttat caaaagaaga gtttatagaa attgtaaatg
    39121 acttagaatt aaaatttcat tctattcaat ctggttttat tgatactgag cttttatatg
    39181 atgaaaagtc tcaaaagtgg attatgatac aacattggga atctatagaa gacctcaaga
    39241 gtgcttctag taaaatgttt aaagattctt caactgagaa atttagaaaa gctttaaaga
    39301 atcaaactgt taagatgaat ataataccac aaattaaaag ttggaaatta tagaactgaa
    39361 tgtaaaagat agttaaatag tatcaaacta aattagccaa ataacatttt gtatttaaat
    39421 tgttatttgg cttttagtgt ttattttaat atgagtgtta tatactagaa aaaccaacaa
    39481 actgttagag tgtggattat cataatgtca tattcgggag ctaaaagtct taatactgat
    39541 ttgccaattg gtcaatccat gcatgtccat ctggatatgc tgttattaaa gcatttacaa
    39601 gccaatccct ttgctcttgt gataatagtg ggactgttac atcgaagtct ttttgataac
    39661 caagcctatc tatgtatatt gatttgcaaa acaaaaccac ttcgggagcc atatatggga
    39721 tattatctga tgttatcagt ctggccttat ccatatctct tataattctt tgattttggg
    39781 aaaaaagaaa ttttccatct ttctgtggat tatgtatcaa ttcaataaaa tcgaactgta
    39841 actgttcgtc acactgtata ttcattttat agttatcatc ctcgactttt tttaattcaa
    39901 taaatgagca atcaggctta atcgcaaaaa cacaaatttg ctctatatgt tgcttgttaa
    39961 ggtcgataat aggtcgtaat aaataattac ccaagggctc atatatatcc catccttgat
    40021 taagcatata ctgaatgctc ttttctttat ctgtgtcaga aatccatatg tcaatatcac
    40081 tgtgtgggcg aatatgcata ttcgcaaata attctagggc atatccacca catattgaat
    40141 atgaaaagcc acaatcatta aaaaagttta cacaatcttt aattagtttg ttcatagcaa
    40201 cctccttttg catgtactga aataaattaa atttttaaaa ataccaacta aatgtttgtt
    40261 ttgtgtaaat ataaaactgc cgatattaag gcagttttat atttactatt tagaaaattt
    40321 attaacaaat ttttgatatt cacacattat cttatcattc ataaaagaaa tacccataaa
    40381 agcagcttgc tcaccaaaca aagtaaaacg ttctatcatc tgttgtggat tacctggata
    40441 aactgttgga atacaccatg tgtttagaag aaaaccaaac atttgagcag caatctctgg
    40501 atttttaact tccatagaat tatcttgatt tccttcttca atcaatgctt ttatccaagg
    40561 cacaatatca tttataatct catcaaactg cgtagctaaa tacttaggat tacgcaatag
    40621 tgatgatgac attaaaaaaa tgtcttgttt attgtgttgt tcataagcat gaagcaacct
    40681 atattgtaat ttttctaatg cggtctttcc ttcatcattt tttacctcat acccataaaa
    40741 tccatcatta tacaaccgac ttgtgattgc cttaattatt tcgtcttttg atttaaagtg
    40801 atgataaata gcacctcttg aaaggccatc taaattatct acaatgtctt gaatcgttgt
    40861 ctgctcatac cctttttcta agaataactt atgtgaaata tcaagtattt tttgtatagt
    40921 attatcaggt tctttttttc ttcccaaaaa gctcactcct caaaacaatc atttggtatg
    40981 tttatcttag caaatacata atgatttgtc aatgtgtctt tttagcatgt tgaacaggta
    41041 tattgccatg ctttcattat tttatttttt tgccttatac tggactatat ttgcagtttc
    41101 ctaatatatt tggctcatat ttaaatacca ttatctaaaa gcactattgc tgtacgagag
    41161 gcaataggca ttttagaaga aggaggagtt gagttaaagt atacatcata atctcttaag
    41221 ttttcttttt ttgtgtttcc tccaaactct accatatcag tatttaatat cgttttgtag
    41281 tttgtatttt gaaactttga aatatcaact gattgtatta gattagggtg gaaattaaat
    41341 ataaataaat atttgccctt actgtacacc aaaatctttt tatcattatc catatgtata
    41401 agttcatgac tattttggat aaaaatatca ctgtattttg taagttcgag catagattta
    41461 tcaaaattta ataattgttt atactttaag ttgttatctt ctgaaaggct ccattgtctt
    41521 ctggcatact tatagctcca attgttgcct tctcgtggaa aatcaatcca ctcaggatgt
    41581 ccaaattcat ttcccataaa atttagatat ccttcacctg ctagagaaaa agttattaat
    41641 ttaattaatt taataagaga tattgctcta ttgataacat ggttatttga gtttatttcc
    41701 atattccagt acatttcctt gtcagctagc caaaaaataa ttgttttatc gccaactaat
    41761 gcttggtcat gggattcaca atatcctata tttttttctc caggtcgtct agtagtaagt
    41821 tcataccaca tttttcccaa atcccagtct tcatcagata agttagagat agtttttatc
    41881 caaaaatcag gtactcccat tgcaagtcta tagtcaaatc caattccgcc gtcttttata
    41941 gggatacaca taccaggcat tccactcata tcttctgcaa tacttatcga attaggttta
    42001 atttctttta ttaattcatt tgcaaattgg agatatgtta tagcttcaat atctgtattc
    42061 atactaaaat atttttcata actatcaaat gaaactccaa gaccatggtt atgatataac
    42121 attgaagtta caccatcaaa cctaaagcca tcaaaatggt actcatttaa ccaaaatttt
    42181 atatttgaaa gcaaaaaatg tattacttca ggtttaccat agttaaaaag ttttgtaccc
    42241 caagcaggat gatttccctt tgaacctgag tggaaaaatt gatgttcact tccatcaaac
    42301 tcatttattc cttctaaagt attctttaca gcatgagaat gaactaaatc taataaaaca
    42361 gcaatcccca ttgaatgagc agtgtttatg aggtttttta agtcttcagg agtaccaaac
    42421 ctagatgaga ttgcataaaa gtttgaaact tggtatccaa aagatgcata atatggatgt
    42481 tccataatag ccataagctg aatggtattg tatccagctt tttttatctt aggaagtatt
    42541 ttttcagtaa attcattata agttccaata gattcgcttt cagtagacat acctatatga
    42601 cattcataga ttagtggaga agttatgttt tttaagtcaa aattattgtc agtccagata
    42661 aatggagttt ttggttgcca tatttgtcca ttaaatccac caaaatcttt ttgaattact
    42721 ctttttatat ataatggtat acggtcaaat gtttttccat ttgcagtaac ttgtaccttt
    42781 acctctgatt tgtgaggtaa gctattttga ccaggaataa atatttccca attaccagac
    42841 ccaatttttc ttagaggatg agattttcta ttccaattat taaagtcacc aattagtgac
    42901 aaactatctg catttggagc ccattcccta tagaagaatc catcatttgt ctgatgaaat
    42961 ccatagtaaa gatgagcatt tgcaaaagaa ctaaaatctt tatagttttt caatgttttt
    43021 gatttcatat cctcataatg tttcattcgc ttttttatat cagattcaaa tggttgtagg
    43081 tatacatcaa tatctaaaat tttaaatctt ttttgacttt tcattgtatt tctcctccat
    43141 aataaatatt acatctaaaa aataatattt atctcatact atcttctctg tataattgat
    43201 atatattata taatcctaat aaaaagctta agccaccaat gattaatttc ataatttacc
    43261 tccctagtat ttatacttat atatcgtatt catcaaaatt taaaagtttg tttaaaactc
    43321 ttttaataat atcataatca aagcctttat aggaaagatg ttgagaaatt ttttgatata
    43381 ttttcttttt atcttctttc tttactcttt catacctttt ttttgctaga tacatagcat
    43441 tttcaaattc agtgtccttg tctaattcag atactgcttc attgattgta cttctattga
    43501 tacctttatt atataaattc tgcttaattc tgtttttgcc acatttattt aggttaacat
    43561 ttgtgttaac aattttttga gctaatacac tgtcatttac gagattataa cttttaagaa
    43621 aatctatgac cctctcaatt atattttcat caaattctga tgatagtttt tcttttaatt
    43681 ttttttcaga ttggtcagca tgagataaaa tatttaatgc tttattttta gcttttatat
    43741 acatttcatc atctaaaata ggttttaatt cttcttcatt tatttcattc ccctttttta
    43801 aattaaatgt ataaacaagt tctttaaata tagcaataaa aaatttatca tctacataaa
    43861 tattaactct atcatcattt cttttttgtt gttctatttt tgtaattata cccataagtc
    43921 ctcctttttt atatgtaatt atattaacaa aagcaactaa taaaatttaa caaaaaactt
    43981 atatttgtat gttatatttt atcaaaatta agacaaaata gtaaaaagtt atttttatta
    44041 aaaagtaaag tagaatcatg ttaaataatt atttttttaa gtgtttttta gcatataaat
    44101 ataattatat acccaaaaaa acaaattata agctttaaga aataaaaaat aatggtacaa
    44161 tagtattata acattaaaag gagacaaaat atgagaagtg aaaatctagt taagatggca
    44221 gagttgaaaa atactgaaaa actagagaaa tttaatcgcc ataaaggcaa aatcaaaata
    44281 ggtatattgg gaggtacttt tgaccctata cactatgctc atttagcaac agcagaattt
    44341 attagagata agtatgacat tgacaaaata atctttatac catcaggaaa tccaccacat
    44401 aaattatgta tcactacaga taagtatgat agatataata tgacacttct agcaactgaa
    44461 agcaacgaag attttttagt ttcaaaagta gaaatcgaaa gaaagaaaag gacttataca
    44521 atagatacat taaaatactt aaagaaaaaa tacaaaaatg cagacattta ttttataaca
    44581 ggtgcagatg caatttgtag tgttgaagag tggaaagatg taaagaaaaa ctttgagtta
    44641 gctactttta tagccgcaac tagaccagga attagtttgt taaggtcaca agagactata
    44701 gaaaaactaa ctaagaagta taatgctgac attataacag tttatgttcc atcactggat
    44761 atatcgtcca catatataag ggaacagcta aatgaaggca agtcaatacg ttacttagta
    44821 cccgaaaatg tagaaaatta tttatatgaa aataaactat atcaatatgg agatgattaa
    44881 atgaacttag aaaatattaa ccaaagacta aatcaaatgc taccagtggg gaggcttagc
    44941 cattcaaaaa atgttgcaaa atgtgctgaa aaattatgtg agatttatgg atgtgacaaa
    45001 gaaaaagctt acttagcagg aatgattcat gattgtgcaa agtatttaag tgacaaggaa
    45061 atagaggatt atgtaaataa gtatgaaata tatttagacc caatagagga tggaaatcgt
    45121 tccttgtctc atagtgtaat tggggcgtac atatgcgagt atgaatttga agtagaagat
    45181 gaagatatta taaatgcaat caaatatcat acaacaggga gagaggacat gtctctttta
    45241 gaaaaaataa tttacatagc tgatttaatt gaagaaggaa gaaaattccc agttgtagat
    45301 acattaagag aattagcata tggagggaag cttgatgaag cacttttaac ttcttttaat
    45361 aatactttaa tgtttgtcat caataaaaaa gaagagatac atccaagaac agttatggca
    45421 agaaactatc taattaaaga aaagttatta taaaaaagct atataaaggc aaacatatta
    45481 ataaatttaa atttgactat gttacaaaat cgtattaata tgttacaata actaagtgta
    45541 ttttaattag gaaagtgagg aatttttata tgacagtgga acaaatgaca aaaatagcat
    45601 atgatgccat agaagataaa ctgggtcaag atacagttat aataaatata ggaaaagttt
    45661 ctagtttgtg tgattatttt attattacta cagcatcttc tcagagacag gtaaaagcta
    45721 ttgctgacaa tgtggaagat gaattagcaa aactaggatt agagccaaga ggtaaagaag
    45781 gacaaggaac tcaaacttgg gtactccttg attatggaga cataatggtt catgtattta
    45841 atgaggaaaa tagagggttt tataatttag aaaaattatg gaaagatgca ccttatatag
    45901 atattgacac attagcataa acatgataat atatgttatt ataattagaa aatttcaaaa
    45961 attatattaa attgaataga ctagagcagt aaaagattat attttttcag agagttagtg
    46021 gaaggtgaga actaatatta tataactttg aacttactag attaaaatct attctccatg
    46081 gctggattaa aagctatcta agagagtcta tatatgtagt aggctaatta gggtggtacc
    46141 gcgaagattt atcctcgtcc ctaaacgtaa gtttagtgac gaggattttt tattttcaaa
    46201 gaattatttg gaaattataa gtaaataata ctaaataagg tagttataca caattaaaaa
    46261 taggaggcaa aatatgagcg tatataattt taaagaagta gaatcgaaat ggcaaaaaat
    46321 ctggaaagat aataatcagt ataaaatgga tactgcacaa actgaaaaac ctaattatta
    46381 cacattagaa atgttccctt atccatctgg aaaaatacac atgggacatg ttagaaatta
    46441 ttctataggt gatgttgtcg caagattcaa aaaaatggaa ggatataatg tacttcatcc
    46501 aatgggatgg gattcttttg gattaccagc tgaaaatgca gctataaaac atggaataca
    46561 tccgcataaa tggacaatgg aaaatataga agaaatgaaa gagcaattaa atttattagg
    46621 gattagttat gattgggata aagaagttgc aacttcaact ccagaatact atagatttac
    46681 acaagaaata tttttgaagt ttttagagca tgggcttgca tataagaaaa aatcatatgt
    46741 taactggtgt ccttcttgtg agactgttct tgcaaatgag caagttgttc aaggagcatg
    46801 tgagagatgt aaagcaacag tattaaagaa agatttagaa caatggtatt ttaagactac
    46861 tgaatttgca gaagaattac ttaatgactt agatacatta gatggatggc cagaaaaagt
    46921 taaaacaatg caaaaaaatt ggataggtaa aagtacagga gcagatttag tttttgatat
    46981 agatggaact gataaatcta tgacagtatt tacaactaga ccagacacaa cttatggagt
    47041 tacttatatg gtacttgctc cagagcatga attagttaaa gaattggttg cagggactga
    47101 atatgaagca gatgttgaaa cttttgttca aaaaatgcat actatgacag aaatagaaag
    47161 aacagcagct gatgtagaaa aagaaggaat gtttataggt agatatgtta taaatccttt
    47221 aaatggcaag aaagttcctc tatggatagc taactatgta ttagttgagt atggaacggg
    47281 tgctataatg gcagttccag cacatgatga aagagataga gattttgctg aaaaatataa
    47341 tttagacata attgatgtaa taacagaaga taacaaaatg ataaattcag aagaatttaa
    47401 tggattagat gcttcagaag gatttgaagg aataatcgat aagttagaaa aagaaggtag
    47461 aggaaagaga acaattaatt atagacttag agactggtta gtttcaagac aaagatactg
    47521 gggttgtcct atacctgtgg tttattgtga tgaatgtgga atagttccag ttaaaaagga
    47581 agatttacca gttcttttac caacagatgt tgaatttact ggaaaagggg aatctccact
    47641 tacgacttca aagcaattta tgtcaacaac ttgtcctcac tgtggaaaac cagctagaag
    47701 ggaagttgat actatggata cattcgttga ttcttcttgg tatttcttaa gatatgtgga
    47761 tagcaataat gaaaatgaac catttagtaa ggaattagtt aatagatggc atccagtaga
    47821 ccaatatata ggtggagtag aacatgctat aatgcattta ctttatgcaa gatggtttgt
    47881 aaaggcattt aagagtatgg gaatggtaga cttcaatgag ccatttaaaa acttacttac
    47941 tcaaggaatg gtacttatgg atggttctaa aatgagtaag tctaaaggga atacagtatc
    48001 tcctatggat ataatagatg agtatggagc agatactgca agacttttcg tattatttgc
    48061 agcaccacca gaaagagatt tggactggtc agagcaaggt gttgatggat gctttagatt
    48121 cttaaatagg gtttatagat tggttgacga gttagcagat gtggttaaaa aggatgtaga
    48181 gtttggagaa ttaaattccc aagataagga tatgagatat actatacact ctacacttaa
    48241 aaaagttact gctgatttaa gtgaaaaatt cggatttaat actgctatat cagctcttat
    48301 ggaattaata aatgatatgt ataaatataa agagttagat aatataaacg aagcagttat
    48361 aaaagaaggt gtacaaacta tagtaactat aattgcacca tttgcccctc atttaggtga
    48421 agaactatgg actatgatag gtaaagaagg aagtgtattt gatatagact ggcctaaata
    48481 tgacgaaaaa gctttagtaa aagatgaaat agaagttgtt gtacaagtta atggaaaagt
    48541 tagaggaaaa ttaactgtta actcaaatat atctaaagat gagatggaaa aggtagcttt
    48601 agaagatgaa aaaataaaag gattagttga aggtaaaact atagttaaag ttgtagctgt
    48661 acctaaaaaa ttagtaaata tagttgttaa ataactcaaa aatatgaaca aaaatataaa
    48721 tttataaaat aaatgaggct gtctcaaaat agagataagc cttttttcat tgttgaaatt
    48781 ataaaaatag atttgatttt atacaaaaaa atgattaaag aactaaaaag ttcattctta
    48841 tataccctag ttgttgtttt aaatattatt ataattaatg ttaaatcgag ttaatgtatt
    48901 aagtggtatg taaatcattc agtttccaaa gtgttgttca tcaaggaact gctctaagct
    48961 taaacgaaaa aaatataatt attgtatttt gaaatttttt tctatataat gaaaacaggc
    49021 aaaaaaggtg catatctatg ctttatatga aacacccaga caacgaaatt cagattcata
    49081 taatagctac catgtttcta aaaaggaact tgtaaaaaag atagcgtttc aacttcccag
    49141 ttgattaaaa ttatcagcag gaaaacaagt atggtcagca gtggtattct aatgggtgtt
    49201 gtcgatatat ccaagttggg attgtctgag gatgttatca aataacttgt gataggtgca
    49261 gtagatgtgg agattcctga tacggattta ttctcagggg atggttgctt cgggagatat
    49321 ggagcgtaac cagtttattg atatggtaac cattttttgt ctaatttgat gaaagaatcc
    49381 caagaatacc atacagaagt aagacaggaa ttaactggtg gtataaactg ataaaggcaa
    49441 atttaaaaat tttgatagga tgattttaag atttatcttc tattttgttt tttagaaata
    49501 attgaaatgt tatgaggagg tatgtacaaa tggagactgt aacaaataat caatcaggtg
    49561 tagaaattca actcagtctg tttgaagcta tcaaagattt attgttaatt aatcctattt
    49621 tcttgttggg aattatcttg attggtattt tcttagttgt aatattcaag aaaaatagtg
    49681 atattccaaa ggtaaaaaca tctattttat cactggcaat gtattactac ttatgtttaa
    49741 cactatcaca tattgttggt atacctacat tcggtgaata tataagactg tcacagttag
    49801 gggaaacgtt ctttaatcca aacataaatc ttattccttt tagtgatgga tttagtttga
    49861 gttttatttt gaatatttta ctttttgtcc cattaggttt tttatgtccg cttataagca
    49921 aaacctttga acgtttgaga aatacattct tgattggttt tggattatct ctttttatcg
    49981 aaactattca gctttttacg ttacacagag caaccgatat agacgactta ctcacgaacg
    50041 taattggaac aataataggc tatttttgct tcaaactgat tgctaaattg cgaattgtaa
    50101 aactacattc agatgatgac tctatggaac aggattacac agtatgtatt ccgattgcca
    50161 ttataatagt tgcccttata ttaggatttt ttagttaagt tccaaatagc taatttggga
    50221 attatggagg tcattaagtg aataaaatta cttttatatg tttaggtgta aaaagtatgg
    50281 agaagtcaat taaattttat agagatggat taggatttta gattacaaac ttttagttta
    50341 ccaaggagat agcaaagtcg tgtgccaaag ggtaaccatt tggaaatcca gacaacataa
    50401 cagatagtat gttacctttt acaggagcat actatctgtt atattgtcaa catcctattt
    50461 tatggtttgt gtgtagtttc ctgtaaactg aactaaataa ttaaatagaa gctattagaa
    50521 ttaataataa cagatagata ttaataagca taaacaaata gtaaaatgtg tagatataga
    50581 cgtttttaag tgaaactatt ataaaataat tgactgataa tcaactgaca aaacctattt
    50641 ttttaggttt aactgacatt taactgacag tcagttttta ctatttaaat agaagttcgc
    50701 ttagattgtc ggaagcttct ttgtccattt ctttaagtac atgagaatat ctattcatag
    50761 ttatttttat atctgtatga cccaatcttt cagaaacaac tttcatatta gttcccccta
    50821 aaagcaacag agtggcactt gtatatctta aatcatgtat tctaatcttt tttaaattat
    50881 tttttttgta aaattgtgaa acgtttttct tagtgaagaa ggtagaaacg gatttaaatt
    50941 ggtgtttaga caaactaagt tatttctgtt ttccaggata ccttacaatt ttaatttgct
    51001 ttgtcttagt ttttcaattt tcaatttttc aattaattct tttggagctg ataaagctct
    51061 ttttgatttt ggtgtttttg gctctttaaa agatattttg ccattagaat agacaagaat
    51121 ttgattaaca tttatcagat tgttatctaa gtcaacatct gaccacctaa gcccagtagc
    51181 ttctcacatt cttaaaccta gaagtaacat taaaagaata ggtatttcta tattattacc
    51241 aagtaacttt tctattagca ataatacttc ttctctattg tatatttcat ttgtagaata
    51301 tttttcttta gaaggaagtt ttataaaatc acatgaattt tcttgtattt ctcttaatct
    51361 atacgcttcc cttattacag aacttagaaa gctatacctt actttagcac tagtaaaggt
    51421 agaattatta aagttataat ttacaaaagc ttgtactgaa ctaggattta cttctgtgag
    51481 ttttagattc ttaaaaaagg ttctatgtaa tttttaaccc aagattttgt atttgtaaca
    51541 gtatatggag accaattatt tttattcatg tctatatatt tataacatct ttctacaaat
    51601 gtcatacctt ttggagcaac aaacttatta ttatttatag tagactttat ttcaattaaa
    51661 tgtttttcag catccttttt ctttttatag cttccataac ttttttgttt ctttttgcca
    51721 gtttcttatt ccacatatac atagaatttt tcccatcttt ttcttataaa agctgatttg
    51781 atattcatat atgtacgctc cctttataag ttagttttat aaaagaacag ttggactgct
    51841 ctaattatgt cattatcttt ttaattcttt acagttacat tataacacgt atttgtatac
    51901 acttaaatta acaggaagat aaatatcatt aaaggaggaa aagaaaattt aatgtagaat
    51961 tatgtaagat atatagtatt tcatatataa aagggggaga tattgtgaaa ttaaagaaat
    52021 tattatcact agggttagcc gtgtcaatgt ttgctgctgg tcctatatca gctaatgcac
    52081 ttgatacaat ttatactatt caaggaaaag ataaatatga aacggctgct cttatagctg
    52141 atgaacaaaa ttatactaca gctatattaa ttaatgcaga cagcacaatg gctgatggat
    52201 taagtgcgag tggacttgca ggagcaacaa atgcaccaat ttttttaact aagaaaaata
    52261 acatacctaa tgcaactctt aaaagagtag agaaagctaa aaaggtatac ataataggtg
    52321 gagaaagttc tattgataaa gcaacagaaa ctcttctaaa agataaagga atagaggcta
    52381 aaagacttca aggtagtgat agaattaaga caagctataa tgttgcaaaa gaaataaact
    52441 caattaacaa agtaaataaa gttatattga ctaatgc
//
LOCUS       DAENCV010000011        51596 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAENCV010000011 DAENCV010000000
VERSION     DAENCV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.011"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..808
                     /locus_tag="KQD82_000575"
     CDS             <1..808
                     /locus_tag="KQD82_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG4379076.1"
                     /translation="DKIVKDTNSTRLGGTDRYDTNKKIINKFYSGVKEFYIASGTDLV
                     YALVGSTVAKNNAIVLVDNDSNKSVLKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNT
                     GREYTVEDAANAVKKLLGVKKFYYEPNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQG
                     AEGDVRYCVAKNDMSKVYTYDVNGNMKVCNPDDYKNKGSITEEQAKQIALKECAKRHK
                     VSINNLVVDMIDFRDDIYSGNVYAVKINSKNSNGTFGWFFIDIETGKVYN"
     repeat_region   1399..1626
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1399..1427
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            complement(1668..1886)
                     /locus_tag="KQD82_000576"
     CDS             complement(1668..1886)
                     /locus_tag="KQD82_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379077.1"
                     /translation="MLKKLRKERGMTQNELAEKANYSRAYISDLENKRYKNINISTII
                     DLSLALEIDFLELCRYYCEEELRKRKLM"
     gene            2643..2819
                     /locus_tag="KQD82_000577"
     CDS             2643..2819
                     /locus_tag="KQD82_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379078.1"
                     /translation="MDEMGKNKIITINLEDEIKSLDLDIFIKYIEIFKKKDNDSFLKT
                     LDYMKYSIKKNKAL"
     gene            complement(2825..3145)
                     /locus_tag="KQD82_000578"
     CDS             complement(2825..3145)
                     /locus_tag="KQD82_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379079.1"
                     /translation="MGLEKINELRKERGLTSEQLSKLSGVPKGTLDKILTGVTKDPKL
                     ETLKSIARVLDCTLDDFDDKPNLNKLDDINNKEIKEFAQLFLEVDDETKEYVIGLMKK
                     TLKK"
     gene            3336..3527
                     /locus_tag="KQD82_000579"
     CDS             3336..3527
                     /locus_tag="KQD82_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4379080.1"
                     /translation="MARNDLNRKKLASKLGVSPKTLSNKLKGNTEFTWKEVKQIRDIV
                     APNKTLEELFRHEKESVNN"
     gene            3648..3815
                     /locus_tag="KQD82_000580"
     CDS             3648..3815
                     /locus_tag="KQD82_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379081.1"
                     /translation="MDGVRHRIKMVSNMYQLDGKPSINTVKNLVSPICWGKMTGVGLL
                     GVIQTDTKIKS"
     gene            3829..4259
                     /locus_tag="KQD82_000581"
                     /pseudo
     CDS             3829..4259
                     /locus_tag="KQD82_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4265..5080
                     /locus_tag="KQD82_000582"
     CDS             4265..5080
                     /locus_tag="KQD82_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG4379082.1"
                     /translation="MNNLQVIERNNKRVLTTQQLADVYETDVRNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EESYFNKKKDTLITNQLSPELQMFKQIFDTVAKQEIEQKQIKQEITETKQEIQSIKDV
                     VTLNTTDWREETRRLLVSMSQNLGGNEYINTLRKESYEFLCKRFNVDLKRRLNNRRRK
                     MAEEGVCKSRREKFNYLDVIQEQNYLIEGYVIVIKDMALKYGISSDLSKNCNN"
     gene            5100..5351
                     /locus_tag="KQD82_000583"
     CDS             5100..5351
                     /locus_tag="KQD82_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379083.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDEEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWKEFDEVAKEVAINIAMKKAETVES"
     gene            5466..5627
                     /locus_tag="KQD82_000584"
     CDS             5466..5627
                     /locus_tag="KQD82_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379084.1"
                     /translation="MLSLDISKKNVMTLKKDGKFLADIVFKDIKTGKKISVGTLNKKS
                     AGQSNQHNK"
     gene            5830..6072
                     /locus_tag="KQD82_000585"
     CDS             5830..6072
                     /locus_tag="KQD82_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379085.1"
                     /translation="METARLIAIGQIKQAEKEISRLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASTEFREFMIIQEELERDRPIDVQL"
     gene            6203..6520
                     /locus_tag="KQD82_000586"
     CDS             6203..6520
                     /locus_tag="KQD82_000586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379086.1"
                     /translation="MNVVKGCNKQLHVTLNSTCLQDNNLSWQAKGLHLYLMTKAENET
                     IYLEDLKNKSSNGRDSTANILNELIEKGYVSREIRRDALTGKVLGGYEYKIYEESLKN
                     IEK"
     gene            6529..7464
                     /locus_tag="KQD82_000587"
     CDS             6529..7464
                     /locus_tag="KQD82_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG4379087.1"
                     /translation="MNFDNETYFHINFEDPFTRVPNAILDNKNLTYSAIGVVTQMLRF
                     QKSGSHKIYAKSLISYRKDSKSRISSALNELINEGFVIRSKIRNDKGQIKGYRYDIFD
                     TPQIKIEEKEDVKSQPCFNIPNTVNPEIGKPGSEKTGDSKTEIQFFGNIKENSIKENN
                     NTIITINKEQEESAYIKKYYETYIGLITPNNFIELAGYLDDGMEVDVIIRAIDEATSN
                     GVKNYRYIKTILNNWIESGVKTNLDLTEYQNEYEKKKRKSNSLGGVKNDKQGKRNSQT
                     KSTEFDFNSKETRINDNELERARQALRDIGVEPRY"
     gene            7400..8137
                     /locus_tag="KQD82_000588"
     CDS             7400..8137
                     /locus_tag="KQD82_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4379088.1"
                     /translation="MTTNLKEQDRHLETLELNQDTEYKCLKCKDRLFIETDEGFKECK
                     CRKLRIAENILKNSGVSEEFRKLKFDNFNYQVDSSVAKAFTKAIEYSNYFEQIREIRR
                     NSIIFMGQVGSGKTHLSLAIANSLMNRSISVIYMPYRDSMTLIKQNIMDEETYRREVG
                     KYKSAKVLLIDDLYKGNITNSDINIMFEIINYRYLNKMPLIVSTEKNFDELLEIDEAI
                     GSRLIEMSKNYLIELKGKRLNYRIYGG"
     gene            8142..8795
                     /locus_tag="KQD82_000589"
     CDS             8142..8795
                     /locus_tag="KQD82_000589"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4379089.1"
                     /translation="MDKNKMLKTLVRKAQSGDELAFNDILNVLDKEIKINASIYAKYL
                     EGIFEKDDLIAEAQAGLWKSIMNYKIEIEYVSAYFQKVIKNNILRLVNKSKTKKFTNE
                     ILVLNKNVDSNEYTELIEFIQDEGLLVDEVIIEKERAEEIEAEKKLQMKIVKRKIRYI
                     PQPQKEIIRMYLKEYDTEKISKKINKSYNAIYSSKYRAVQNLKEMCLPEISKYNITI"
     gene            8873..9790
                     /locus_tag="KQD82_000590"
     CDS             8873..9790
                     /locus_tag="KQD82_000590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379090.1"
                     /translation="MITAFKTKEEREKEVLEKALNSFSGIQKDRARIYLTENKVNDRL
                     FLSNLLVKYKFEIRDNLKDMERIRDEIREYKSYLKQELGNKTKIKEYINYLEGLLDSC
                     ESLICAYGNALIPLFDSINKEFTKKEICQLIGANEKILDEYYEFMKKHSKEEMSKDFN
                     FIYELIFVHHGEYRWRKGRYKDFVDCPDWEMPLFNALWSHMDNFIENNKEIQESMHSV
                     FDEVFGDISYFMNGDGTMEKSVQEINIKELISNYRDYGNSTVVFQLKKKSIIDITINY
                     KLKRQRGDVLIYKVFDSEDNELGEVHKII"
     gene            9833..10312
                     /locus_tag="KQD82_000591"
     CDS             9833..10312
                     /locus_tag="KQD82_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4379091.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHIN
                     LALSEAKEVNYLEEDLSDFEDEELYILSIKKSKTRTIIMIAHIDSALKTLKLRQERLQ
                     SYEKYRALELYYFDEKTYEEIAEILNCGVVTSRRWVNEMITELGIYLFGVDGLKSIV"
     gene            10460..10663
                     /locus_tag="KQD82_000592"
     CDS             10460..10663
                     /locus_tag="KQD82_000592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379092.1"
                     /translation="MNMINLREKIIKELNEFNIKADNVFLEYAVKHIENFMNEGIDDE
                     ELLIRGVVLGASYVVNIENKDLD"
     gene            10698..10944
                     /locus_tag="KQD82_000593"
                     /pseudo
     CDS             10698..10944
                     /locus_tag="KQD82_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424164.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11151..11363
                     /locus_tag="KQD82_000594"
     CDS             11151..11363
                     /locus_tag="KQD82_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379093.1"
                     /translation="MKCPNCGSENIEQGISTVSESGFVIYRTGLEYNTRFSTKVSKTY
                     SDLCLDCGEIVRTYIKGETDRNWCKD"
     gene            11885..12904
                     /locus_tag="KQD82_000595"
     CDS             11885..12904
                     /locus_tag="KQD82_000595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379094.1"
                     /translation="MSIKVKVWAKLPYIIKLEEEKDYEFSFLFDDFDLFKLNMRFNDE
                     NDFLIYNNEYPGNVCQHIELEVIYKYVDYTKYKTTKCINSNDNYFEVEVPLDKTTEIF
                     GCMNKRLNEILNFLREKSNMFWIDEFNLTHINYHSENEVRFNFYSPNVNPSSVVTSFI
                     EHTDYYMISKNFKTNNVGEDTFKNFDSNKIKMKVFNEYIDKAEKALYEYNYENFIIYC
                     SISVESFIIQYIDEIANQDDIIYKQLEKTNSNYINLYYNVLLKYLTGKSLIEVDKKAC
                     THLTRLYRLRNSLMHKGIIDSKSLEKAGIMKLDFNECNKILDSAKTSFKLVKSITVKP
                     KNDIN"
     gene            12986..13411
                     /locus_tag="KQD82_000596"
     CDS             12986..13411
                     /locus_tag="KQD82_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379095.1"
                     /translation="MAKYEYWITEEGLIKIEGWARDGLTDEQIAFNIGINVKTLYDWK
                     KKYSNICNALKKGKEVIDRQVENALLKRALGYEYDEITYEEGQETKRVTKQVVPDTTA
                     QIFWLKNRKPAEWRDKRDIEHSGNLGDITIKVGDEEYGN"
     gene            13401..14642
                     /locus_tag="KQD82_000597"
     CDS             13401..14642
                     /locus_tag="KQD82_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG4379096.1"
                     /translation="MAINLEINPDVFNPIYLKHQLNNNNRYQIYFGGSSSGKSFSLAQ
                     RTVLDVFKGNRNYLIVRNVQSTLKRSCLNEITKAINNFKLNEYFQVNKTNMIITCKLN
                     NKQILFCGLDDVEKVKSITPIDGVITDIWVEEATETDYKAVKQLDKRLRGKSKVVKRL
                     TLSFNPILKDHWLYTEYFDIWEDDKQYVEKDNVSILKTTYKDNKFLAEDDIKALENES
                     DKYYYEVYTLGNWGVLGAVIFKNWRVEDFSDIESTFDNFRHGIDWGFADDPFAYVKSH
                     YDRMRRKLYICDEIEAVGLLNREAAPLVSKKANGDLVICDNASPKDVAEFSDLRINAV
                     SARKGAGSIEYGIKFLQGLEIIIHPRCQNFKNEINKYKYKEDKNGNILPIAVDKDNHL
                     IDALRYSLENDMEYGGISFLK"
     gene            14647..16086
                     /locus_tag="KQD82_000598"
     CDS             14647..16086
                     /locus_tag="KQD82_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG4379097.1"
                     /translation="MLNIYISETDLIKAQLKKESTFNLAKVIEHYILKHRPEKYQEGE
                     AYYYGNANIKNKRRYYILDGAKVDDFTKVNNKAVNNYHKLLVDQKVGYSVGKPIVFNA
                     DDNNFTKLLNELLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDNKRQNEVVAFIRFYFIEDIDGNKIKRVEYYTENEVIYFIEKGNSFVQELLYDEYG
                     KLTEIQEGHFRVNNKEQGWGKVPFIPFKNNEKSVSDLTFYKSLIDIYDNNISTLADNL
                     DEVQEAMYILKEYGGTDLVEFANNIRYYKAIKVGANGGVDKLEINIPVEAKKELLNRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDEI"
     gene            16076..17473
                     /locus_tag="KQD82_000599"
     CDS             16076..17473
                     /locus_tag="KQD82_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG4379098.1"
                     /translation="MKYKDYWRKRFEQLEEVQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LSWYNRFTESEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRLALVNALHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPFFSDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            17488..17688
                     /locus_tag="KQD82_000600"
     CDS             17488..17688
                     /locus_tag="KQD82_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379099.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            17754..18317
                     /locus_tag="KQD82_000601"
     CDS             17754..18317
                     /locus_tag="KQD82_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG4379100.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYANI
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            18329..19228
                     /locus_tag="KQD82_000602"
     CDS             18329..19228
                     /locus_tag="KQD82_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG4379101.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            19228..19461
                     /locus_tag="KQD82_000603"
     CDS             19228..19461
                     /locus_tag="KQD82_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379102.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            19471..19872
                     /locus_tag="KQD82_000604"
     CDS             19471..19872
                     /locus_tag="KQD82_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379103.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            19866..20213
                     /locus_tag="KQD82_000605"
     CDS             19866..20213
                     /locus_tag="KQD82_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379104.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            20213..20503
                     /locus_tag="KQD82_000606"
     CDS             20213..20503
                     /locus_tag="KQD82_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG4379105.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            20613..21419
                     /locus_tag="KQD82_000607"
     CDS             20613..21419
                     /locus_tag="KQD82_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379106.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            21768..22205
                     /locus_tag="KQD82_000608"
     CDS             21768..22205
                     /locus_tag="KQD82_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379107.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFICPLNP
                     SKTSYPNGRTLKKNSFDVHYFPRSKDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTEKMNDVELFGGLKRGDNFE"
     gene            22198..22374
                     /locus_tag="KQD82_000609"
     CDS             22198..22374
                     /locus_tag="KQD82_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379108.1"
                     /translation="MNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEEIINNFMKGVS"
     gene            22375..23685
                     /locus_tag="KQD82_000610"
     CDS             22375..23685
                     /locus_tag="KQD82_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG4379109.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEIFTVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDTTLEASAGLVFTGGTNGESVTGAEYQAFLDKIESYSFNALGSLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKVKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            23702..24172
                     /locus_tag="KQD82_000611"
     CDS             23702..24172
                     /locus_tag="KQD82_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG4379110.1"
                     /translation="MFQQIKARDTISASKAECYVTIEGKRYNFMQAINLEAKMEKNKS
                     EVPILGSTTKGNKSTGSKYTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SSAGRQTIILKDCNMDSGIIVKFDADGEYLDEDMDFTCEDWELVEKFNIINGME"
     gene            24243..24683
                     /locus_tag="KQD82_000612"
     CDS             24243..24683
                     /locus_tag="KQD82_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG4379111.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYIASDRFLDEEGKPVEWELKVLSSE
                     EDEVLRRNCTKRVKVIGNNGKPTGQFTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTTFEDKVEEAKN"
     gene            25026..25187
                     /locus_tag="KQD82_000613"
     CDS             25026..25187
                     /locus_tag="KQD82_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379112.1"
                     /translation="MDNFLIGVLASLTASLIGYIVCLCIKKVKSHSVQESDLDVELKF
                     SFKFKKNKH"
     repeat_region   25469..25827
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=25469..25497
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            25897..26094
                     /locus_tag="KQD82_000614"
     CDS             25897..26094
                     /locus_tag="KQD82_000614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379113.1"
                     /translation="MNKDIEFIACSMRLKILIQAREDLIENINKYSTKSYEKNIDNYK
                     KLDMIFEDAIKFEAILLSSLK"
     gene            complement(26069..26434)
                     /locus_tag="KQD82_000615"
     CDS             complement(26069..26434)
                     /locus_tag="KQD82_000615"
                     /inference="COORDINATES: protein motif:HMM:NF022172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HBG4379114.1"
                     /translation="MKRDLELIRDILIKMEESDADRMSISDFMTDVYDERTISYHLQL
                     LLDVGFIEATPMGVQRCLYKHYIVKRITSFGYDYLDNIRDDTVWNKTKKQLGNFASSA
                     SLKVIGSVASSVILKMIGV"
     gene            26922..27110
                     /locus_tag="KQD82_000616"
     CDS             26922..27110
                     /locus_tag="KQD82_000616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379115.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            27231..28097
                     /locus_tag="KQD82_000617"
     CDS             27231..28097
                     /locus_tag="KQD82_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBG4379116.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPAIRQHGAYITNNADPQALREKANEIESLDTVNKTIEILT
                     PFLDNAGIDEKAKLLTAKTIYKKAGIELPLEIEEKEHFFDTVQIATKLNVYSKSNKPA
                     FHAIGEIIKKLDIQDNEKLVVLESKRGWSGSVNKYSQSVIDKIRHWIEENNRPTKIQG
                     EKKNFHVVYKIE"
     gene            28152..28307
                     /locus_tag="KQD82_000618"
     CDS             28152..28307
                     /locus_tag="KQD82_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379117.1"
                     /translation="MCENLLDMDRIELIRELGSIFEKMRNENPDKFYRFVSLVKEECR
                     KKEEKNK"
     repeat_region   28405..28764
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=28472..28500
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            28898..29422
                     /locus_tag="KQD82_000619"
     CDS             28898..29422
                     /locus_tag="KQD82_000619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379118.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            29600..29788
                     /locus_tag="KQD82_000620"
     CDS             29600..29788
                     /locus_tag="KQD82_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379119.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            29906..30727
                     /locus_tag="KQD82_000621"
     CDS             29906..30727
                     /locus_tag="KQD82_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG4379120.1"
                     /translation="MKNELMMFEEKKVEVLEYNGQVLFNPYDCGRCLELSDSAIRNHL
                     SKMNDTQAVLLKNSNVLDKDFRKLHNTGEKFLTESGVYKLIFKSKKEEAERFQDWISD
                     EVLPAIRQTGAYITNNADPDKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLL
                     TAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEE
                     NEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            30790..30966
                     /locus_tag="KQD82_000622"
     CDS             30790..30966
                     /locus_tag="KQD82_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379121.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            31332..32105
                     /locus_tag="KQD82_000623"
     CDS             31332..32105
                     /locus_tag="KQD82_000623"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG4379122.1"
                     /translation="MGLFSGNESCCICGEKGKQKISDGVICSECLKKYKDTFSIVEPT
                     DVIKKISSEEIKKSIRLTLENKKRFESFNASKKVGIYLLVDENKKQLIISDKISNLNK
                     NKRVYDFRDIISFELLEDDESIIKSGLGGAIGGGLLFGETGAIAGSILGKKKIKTYVN
                     SLKIKITINNIKNSTKYIYLINSKISTNSSLYKESYNYAQEILSTLSIITSSKSIEDK
                     KESISSSTADEILKYKNLLNMEAITQEEFDAKKKELLNL"
     gene            32172..34460
                     /locus_tag="KQD82_000624"
     CDS             32172..34460
                     /locus_tag="KQD82_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG4379123.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADEQQKRFNDDVRKGASSTDKLVENAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLAMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSVLLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLEVDIGKIRGMASEGMLTADIVKNSLLSAAEQTNAEFE
                     KMPYTFGQIWTSIKNNAVMIFGVIQKKIEQSMSSRGFRTFIDNFISGLYVLGNVFFNI
                     FNGIISILGSPAFQSFSNTMIVGISLISQALGWIVTQALNLANIFAQNWSIIGPIIAG
                     VVGILGTYLIALGSIWLWETLCKISKNALAIASAIHALAIHAELTETQMATVAQWGLN
                     AAILACPIFWIVAGIIALVAVLFVGVAIFNHFAGTSVSAIGIVAGAISVAAAFVGNLF
                     IATGNLIIDIVALIYNSLANFAEFFANFLDDPIGSVIRAVSRMADAVLGILRSIASAI
                     DTIFGSNLANAVSGWQDKLQGWTDKVAGEAKIKVELMDPNKLHFDRFNCGKAWDAGYK
                     WGDKLETNIKDKFDISKIAEKAKKDLGLDDLWDDKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDTDVD
                     GIVNVLTEKLNDAMIVSAEGIV"
     gene            34476..35165
                     /locus_tag="KQD82_000625"
     CDS             34476..35165
                     /locus_tag="KQD82_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG4379124.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            35158..37050
                     /locus_tag="KQD82_000626"
     CDS             35158..37050
                     /locus_tag="KQD82_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG4379125.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYNNQTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            37064..37324
                     /locus_tag="KQD82_000627"
     CDS             37064..37324
                     /locus_tag="KQD82_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG4379126.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            37329..37748
                     /locus_tag="KQD82_000628"
     CDS             37329..37748
                     /locus_tag="KQD82_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG4379127.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGDIKAEKVVSV"
     gene            37749..38798
                     /locus_tag="KQD82_000629"
     CDS             37749..38798
                     /locus_tag="KQD82_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG4379128.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINEVKSNL
                     TINSNSIVVRCEVVG"
     gene            38791..39408
                     /locus_tag="KQD82_000630"
     CDS             38791..39408
                     /locus_tag="KQD82_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG4379129.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSN
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            39420..40196
                     /locus_tag="KQD82_000631"
     CDS             39420..40196
                     /locus_tag="KQD82_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG4379130.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASITGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELNSKVGDTTLLTTTDKTNIVNALNEVKKTSVDSIETTAEKTSYN
                     NATSKLNATNVQGAIDEIVAEVRGNRSSIISSINDNLIPM"
     gene            40212..41309
                     /locus_tag="KQD82_000632"
                     /pseudo
     CDS             40212..41309
                     /locus_tag="KQD82_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41688..42035
                     /locus_tag="KQD82_000633"
     CDS             41688..42035
                     /locus_tag="KQD82_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379131.1"
                     /translation="MNRGNRIIYNQDGKILLQTGEATGDILEHDTITELNFIDLEFGS
                     IDYSKQYIESINPVTKEPVLKNIEIVLTNEQKRLEVLEKELSMLKEENKNRDSEIVNT
                     AFEIENIKLNNNI"
     gene            42047..42217
                     /locus_tag="KQD82_000634"
     CDS             42047..42217
                     /locus_tag="KQD82_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379132.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTSL
                     LKKEEIPAEPTV"
     gene            42353..44128
                     /locus_tag="KQD82_000635"
     CDS             42353..44128
                     /locus_tag="KQD82_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379133.1"
                     /translation="MATVYEFNYTGSEQRATLKPGKYKLECWGACGGRYKADDFTTCA
                     KGGYAKGEIILKEKTNFRICVGQSGYEKVLSTSSFTRTGYNGGGAGGYSNFAPYKWSF
                     DGGGSTDIRLSSGNSSWDDLEGLLSRILIAGGGGGASESSVSKLGHGGGLNGDNGIGY
                     QGNFAGGGTQYQGGINNSSDFDGLFGKGGSYNKHIGGGGGWFGGAGCVTTQSAAGGGS
                     GYALTKDSYKPQGYIPSSEYWLENVVMTAGGNTTKADGYAKITLLQALPFLNISSYNS
                     TQAIFKADHTDPTLLTKIEYFIDDTLKETITTDLTTEKTINYTLEDNALHTLKIVVTD
                     NANATAEKVVSISKNIMPLEDGASLQDIATKVSEMKDGLKSGKRSIVNVLTLKDIDAS
                     LNNTFVELSEKVKDYFDNADYFFQEANKGKELIATTIGSPATKENTYQELSSYITEFK
                     NTIGTLTTQLESANNKLTDPYITKVNNRGVPSTFTVDFRMYYPSSSFTRRSTCIYVDA
                     TFIHYTTPGDMSTGVHKSVKYVKTDTKDLNGYRIFYDGDGTLSLTYENNSVRVVIQGG
                     YTSSPGRKKSDVYSYNVRMVNTYWD"
     gene            44232..44522
                     /locus_tag="KQD82_000636"
     CDS             44232..44522
                     /locus_tag="KQD82_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379134.1"
                     /translation="MEEISINLLCAVAGVIISYLAFRNSSNRKIQDETETTTKLEQQT
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            44526..44783
                     /locus_tag="KQD82_000637"
     CDS             44526..44783
                     /locus_tag="KQD82_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG4379135.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKEENK"
     gene            44783..45589
                     /locus_tag="KQD82_000638"
     CDS             44783..45589
                     /locus_tag="KQD82_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4379136.1"
                     /translation="MKVVVVPGHTLTGKGTGAVGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCEIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDARGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVVGGGACEKIGSITKEHYTMIKGNDRFDTLYKALDFIDR"
     gene            45842..47101
                     /locus_tag="KQD82_000639"
     CDS             45842..47101
                     /locus_tag="KQD82_000639"
                     /inference="COORDINATES: protein motif:HMM:NF014516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4379137.1"
                     /translation="MRKLDNIKKGTIILDIIPNSANGRDAMKIEKIEIKGIGGIKELS
                     LRFNKGLNIICGANGIGKTTILEVISHLFSIQSSDLKKNAKFDLGEAIADYSFEDIEA
                     VHKSTYQINDFNASVKELIFDNRDKKKYSKFVLFFKTHRELNYSKLDGITGDETYSDD
                     STSYMADLGIDSSKIKNWFINRLLFSKQEGSLTKEQLCNLDLAIEMISILDKNVSYSR
                     IVSDSLDLMINTPQGEIYIEYLSSGYKSCFYILLGIIKELEYRFTKPYIEVKDFDGVI
                     LIDELDLHLHPEWQVKIVNALKVLLPKAQIIATTHSPNMIQTLSPDEIIPLTMDENGN
                     VRKKDLELGEYGLQGWTIEEILTDVMGMKTTSSELYLDTMKKFDRAMDKENVDEIKKY
                     YDILMKMLHPNSTLRTILKIQVAGIID"
     gene            47098..47856
                     /locus_tag="KQD82_000640"
     CDS             47098..47856
                     /locus_tag="KQD82_000640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379138.1"
                     /translation="MIKINRTRKPIELTEEVQKQLTEEFKNNKEKSVWRKKYITNALL
                     EMSHGKCCYCEMKLIEEGKSLNVEHFHHKDKYPDEVVKWENLLPSCGRCNSEKGTHDT
                     KLEPIINPAINNPKDYLYLYNYRYKSKNRNKLGSDTIDILYLNDTDKLIKPRVNICMA
                     LFKRLESIKTFVEDYQNGTGTHTRRKNKIINEVKGVLRCAQPTEEYSAIVANVIVNDD
                     DYKYIKEIMSEFNLWDNELEELESKAKEIMLDSE"
     gene            complement(47929..48417)
                     /locus_tag="KQD82_000641"
     CDS             complement(47929..48417)
                     /locus_tag="KQD82_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379139.1"
                     /translation="MSLKYWFKNIKKKKQIKKDIKYFKGNKESVINYQEYYTKIYNHY
                     NEIKNINTIRKIEIEQTRIKGQLEKNNNPGNALFFSLMAVVFSLLANLFLSSLNIKDN
                     YINLILQSSSIIVLIFIAINTSLDIAVPYRDEIQFYTTALDVLEDMKKKKLKERKYKK
                     RR"
     gene            complement(48752..49135)
                     /locus_tag="KQD82_000642"
     CDS             complement(48752..49135)
                     /locus_tag="KQD82_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379140.1"
                     /translation="MRKKLFLGIFIVLVIAAIVVLNIKNDKNIFIGESEHWTAKFVTT
                     EEQSELEIKYKDSKDVVKQKFNYSYEFIDGEDAIIILKDAVLESGQEKIIKRQRPGLY
                     SNRESNYKVIISWNKNEETINLKKK"
     gene            49282..49611
                     /locus_tag="KQD82_000643"
                     /pseudo
     CDS             49282..49611
                     /locus_tag="KQD82_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49810..50025
                     /locus_tag="KQD82_000644"
     CDS             49810..50025
                     /locus_tag="KQD82_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379141.1"
                     /translation="MYINQTGFVSNGLGIKQQQRKDPTSNSFSYNAPSSWKPVGRDSL
                     QYNCGFESIATLVRGGSSWSFNFIVQP"
     gene            50372..51538
                     /locus_tag="KQD82_000645"
     CDS             50372..51538
                     /locus_tag="KQD82_000645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379142.1"
                     /translation="MLLGDEIKLNLEPSDLINSIPYFKSEYSDLCGLDADFIKEQLLK
                     AENNDRIVSMARGEGRAFVVEPIIKRNNYCKFRNNSYDIFNSIPSYEYAIKIIERYKN
                     HIFNPTEYRNSVMRRVGTYENDIFNWGYKSMHSYMESLPLREFLEPNKVDRTLLFEFL
                     DFMRELFSYQLRTYPGRFDNSKTLDAIETIKNAIIKTESHDDLLIIKEWLCFLLHTGN
                     HRVFKNITPWISLSTGSKRYNIVHKFASRKPFTSYRTISEKHTYNEKEKFIFIDYWEY
                     VDKENLTFRNTNYIVNKLKSMGVNWYENRNNEVMVKYALFPQQIIGYYYFENRKLQYY
                     FINPHYLKAWQKNKDFKIGDELYINQEQVVFPFNNPYRVIYGKKYGHISVVDKR"
BASE COUNT        21190 a         5285 c         9255 g        15866 t
ORIGIN      
        1 tgacaaaata gttaaggata ctaattcaac tagattaggt gggacagata gatatgatac
       61 taacaaaaag ataataaata aattttatag tggagtaaaa gaattttata tagcaagtgg
      121 tactgattta gtgtatgctc ttgttggttc tactgttgct aaaaataatg ctattgtgtt
      181 agttgataat gatagtaata aaagtgtatt gaaaagtaca attaaattaa ctgctatagg
      241 aaatttgagt gatagtatat tacagcagtg tttaaatgta acaaaaaata taggagattc
      301 taatactgga agagaatata cagtagaaga tgctgctaat gcagtgaaaa aactattagg
      361 tgtgaagaaa ttttattacg aacctaatta ttcagaagat gaagatttaa tatttctact
      421 agataaaaat tttgattttt caggaaaaga gtactatgta tttgcttaca ttaaacaggg
      481 tgcagagggt gatgttagat actgtgtagc taaaaatgat atgagcaaag tttatacata
      541 tgatgttaat ggtaatatga aagtatgtaa tccagatgat tataaaaata aaggaagtat
      601 aacagaggaa caagcaaaac agatagcatt aaaagaatgt gctaagagac ataaagtcag
      661 tatcaataac ttagtagttg atatgataga ttttagagat gatatatata gtggtaatgt
      721 ctatgctgtt aaaatcaatt caaaaaatag taatggtact tttggttggt tttttataga
      781 tatagaaact gggaaagttt ataattaaaa ttagaacttt taaacaagtg tttttaatta
      841 tataatttac cataatatgt tataatattt ttagcaagaa gatttaactt acaatctata
      901 gagtggagtt catgtacaaa agattatcct cccaacgatt cgaagggagg tgagttcatg
      961 gataactttt tgtttaatgt tttagctagt ttaacagcta gtgtggtagt ttacttaatc
     1021 agtaaactat tcaaaaaagc aaaaagccac tctcgcacaa agagtgactt acgagttgaa
     1081 tttaaattta tatttaaatc caaaaaataa actctgttta tgatgaactc cactctaacg
     1141 caaaatagat tgtagttctt cttgctttta ttatatcaca aattagtaca gatattcaaa
     1201 aataatattt tttttgatat aataaaaatg tagagatttt gcagtgagcg atatttttga
     1261 taaaataggg cttaacagtt gagatatgag gcattgaggg tatgtgataa atcttatcaa
     1321 ttgcactact catggttcac tgcaaatttg agagagttgt atatgtgtag atattgaaaa
     1381 tacttagttt attttgaggt tttatattaa ctatgtggta tgtaaatacc ttaaaatcgt
     1441 cagatatata acctttttct ttatgtttta tattaactat gtggtatgta aattttatat
     1501 taataaaatc atttacattt aaactcaaca gttttatatt aactatatgg tatgtaaatg
     1561 accaagatac ttgaaaaaat atcgctgact attttgagtt ttatattaac tatgtggaca
     1621 agaatagagt agtttgaact actctatttt ttatttaaaa gtatatttta cattaatttt
     1681 ctttttctta attcttcttc acaataatat cgacacaatt ctaaaaaatc tatttctaaa
     1741 gcaagtgata aatctatgat tgtagaaata ttaatatttt tgtatctctt attttccaaa
     1801 tcagaaatat atgctctact ataattcgct ttttctgcta attcgttttg agtcatacct
     1861 ctttcttttc ttagtttttt tagcatatat aaaatctcct tttattaaat tttatataag
     1921 ataatatttg tagaattata aaatatatga tgttattagc gtcattattt tatggaaatt
     1981 tatggtagaa ttagttaaga atattaagaa aatttatttt gaaggtctat aaataccgac
     2041 caaataattt gtaaattata tgtaaaaata atgcaaaaaa ttgtaagaaa aaaatcaaaa
     2101 ataaaatgat ttatttttag aaaatcatgt ttagtcattt ttagcgttat ataacattaa
     2161 tgtttattaa cgagtattaa atagtactag tttatttaaa atcaaaacta aaatgaagag
     2221 gtgtagagtt tttttgtggg ttgcaggaat ttttggaagt ctatataata tatttacaaa
     2281 gtaattctta gaaattttaa atagagtatt tactggaata aatttaccag agttataaat
     2341 taaggggatt ttcataccta tttctacgaa cataagttct taaaatctta atatttctta
     2401 attttagata aaaataggag atataaaacg tttaaagaat acaagagaat aaagaataaa
     2461 tatataagga gatgcttcaa atgaaaatat aaataatgta ctaaatttat acaaataagg
     2521 aaataatata tagtgtattt aaaaataaaa agactggtgg tgtttaatat taggtgtatt
     2581 taaataatta tttacaatat taggggtaat ataaaatatt tatgagttgg aggaatgtag
     2641 caatggatga aatgggaaaa aataaaataa ttactataaa tttggaagac gaaattaaaa
     2701 gtttagattt agatatattt ataaagtata tagaaatttt taagaaaaaa gacaatgata
     2761 gttttttaaa aactttagat tatatgaaat attcaattaa aaaaaataaa gcactttaag
     2821 tgctttattt ttttagtgtt tttttcatca atccaatgac atattctttt gtttcatcat
     2881 caacttctag aaataactga gcaaattctt tgatttcttt attattaata tcatcaagtt
     2941 tattcagatt aggtttgtca tcaaaatcat ctaatgtgca atctaatact ctagcaattg
     3001 atttcaaagt ctcgagtttt ggatctttcg taacaccagt caatattttg tcaagtgtac
     3061 cttttggaac accagataat ttagacaact gctcagatgt aagacctctt tcttttctca
     3121 attcattaat tttttcaagc cccataaaaa gattactcct ttctagataa taataatata
     3181 taatatttcc attgtcaata ataatatttc cattaacgga aaaaataatt ctaaaaaaag
     3241 gttgaatata tccgttgaag gtgatatagt aaaagagtaa tatccatcaa cggaaaagga
     3301 aggtgagaga attgtacaga gagctactag gtgagatggc aagaaatgat ttgaatagaa
     3361 aaaaattagc atcaaaatta ggtgtatctc caaaaacact ttctaacaaa cttaaaggta
     3421 acacagagtt cacatggaaa gaggtgaagc aaattagaga tatagttgca cctaacaaaa
     3481 cattagaaga gctttttaga catgaaaaag aaagtgtaaa taattaggag gaagatgtta
     3541 tgaaagaatt atcaaatgta acaaccaatg aagaacttgg agttaaagtt aaattaagta
     3601 tgaagatgga agtataggaa ttaatgtaga agatacagct atagggtttg gatggtgtca
     3661 gacacagaat aaaaatggta agcaatatgt atcaattaga tgggaaacca tcaataaata
     3721 ctgtaaagaa tttagtttcc ccaatttgtt ggggaaagat gactggagta ggtttattag
     3781 gtgtcattca aaccgacacc aaaataaaaa gctagggagg aagaaattat gagtaataat
     3841 ctacaaatat ttaaaaataa tgattttgga gaaataagaa cagttgaaat tgatggtaaa
     3901 ctatattttg tagccacaga tattgctaga tgtttaggtt ataaagatac aactaatgca
     3961 attaaacaac attgtaagtg ggtggtgaaa caccacatac cacatccaca aagtaaaact
     4021 aagaccttgg aagtaaatgt aataccagca ggtgatatgt atagattaat tacaaatagc
     4081 gaattaccaa gcgctgaaaa atttgagcgt tgggtatttg atgaagttct accatcaata
     4141 agaaaaacag gttaaagtac atagaaacaa aaaatataca attaatgttt ttctcgaatc
     4201 taagtactga atatcttttt gaaattgaag atgaagttaa agaaaaatat agggggtaac
     4261 aaacatgaat aacttacaag taatagaaag aaataacaaa agagttttaa ctacacagca
     4321 actagcagat gtatatgaaa cagatgtaag aaatataagc aacaatttta acaataacaa
     4381 agatagattt attgaaggta aacattattt tttattacaa ggtgatgatt taaagaattt
     4441 taaaggtatt catacagaat atgaaaacct aaaatttact tcaaaaatgt atctttggac
     4501 tgaaagagga gcaaatagac actgcaaaat attggatact gacaaagctt gggagcagtt
     4561 tgataactta gaagagtctt actttaacaa aaagaaagat accttaatta caaatcaatt
     4621 aagtccagaa ttgcaaatgt ttaaacaaat atttgatact gtagcaaaac aagaaataga
     4681 gcaaaagcaa ataaaacaag agataacaga aactaaacaa gaaatacaaa gcataaaaga
     4741 tgtagttaca ttgaatacaa ctgattggag agaagaaaca agaagattat tagtatcaat
     4801 gtcgcaaaac ttaggtggaa atgaatatat aaatactctt agaaaagaaa gttatgaatt
     4861 tctttgtaaa agatttaatg ttgacttgaa gcgaagatta aataatagaa gaagaaaaat
     4921 ggcagaagaa ggtgtatgca agtctagaag ggaaaaattt aattatctgg atgttataca
     4981 agaacaaaat tatttaatag aaggttatgt aattgtaata aaggatatgg ctttaaaata
     5041 tggaatatca agtgatttaa gcaaaaattg caataattaa ataagtataa gacaagttta
     5101 tgagctcata ttttataaca aggggtgatg ttatgaaaat actacaacaa tttaaaccat
     5161 acaaatatga tgaagagaaa aacatagaat atgatgaaaa aattatagct aatcaaaaca
     5221 cagaagttta tatagtatat ccacgtatat ctgatgaaga gaataggaga agatggaaag
     5281 agtttgatga agtagccaaa gaagtagcta ttaatatagc aatgaagaaa gctgagacag
     5341 tagaaagtta agaaagtaga gaagttgcta atacttagca ctttctcaaa gaagaaaatt
     5401 ggacaactat ttttaaacaa ctaggaataa ataagcgata gtatgtttga aagaaggtga
     5461 tgaaattgct tagtcttgat attagtaaga aaaatgtgat gactcttaaa aaagatggta
     5521 agtttttagc agatatagta tttaaggaca ttaaaactgg taaaaaaata tcagttggaa
     5581 cattaaataa aaaaagtgct ggtcaaagta accaacacaa taaataaaaa aatccaaata
     5641 gagtataaca caaaaagggg gagatttaaa ataggaaatc taacatttta aaagatttaa
     5701 atatgtcata atgatagcaa tttagaatga attaacttta ggttatgctg ctaaagaaag
     5761 gaggtgtaac aattattaac tttagaagat tcttagaact gtaattatga taaattagga
     5821 gggatttaaa tggaaacagc tagattgata gcaataggtc aaattaaaca ggctgaaaaa
     5881 gaaataagta gattacaagg tacaaaaaat aatagtagtt taatgtggtg ggaagccgtg
     5941 aaatttgcta gccaaaatat attagagggt cttgaacatg atattgagtt agaagcatca
     6001 actgaattta gggagtttat gataatacaa gaagaacttg aaagagatag accaatagat
     6061 gtccagctat aagaaaagag cctatgccaa ggctcaattc agtaaaacaa taaattatat
     6121 ttattaaatt atatatcaaa acaataatat tatgcaaata aaaaattaga ttatacatat
     6181 agtagaaagt ttaggtgata ttgtgaatgt tgtaaaaggg tgtaataaac aattgcatgt
     6241 aacattaaat agcacttgtt tgcaagataa taatctaagt tggcaagcta agggtcttca
     6301 tttatattta atgactaagg cagaaaatga aacaatatac ttagaagatt taaaaaataa
     6361 aagtagcaat ggtagagatt caacagcaaa tattttaaat gaacttatag aaaaaggtta
     6421 tgtaagtaga gagataagaa gagatgcctt aacaggtaaa gtacttgggg ggtatgagta
     6481 caaaatatat gaagagtcac ttaaaaatat tgaaaagtag gtgatatatt gaactttgat
     6541 aacgaaacat attttcatat aaattttgaa gaccctttta caagggttcc aaatgcgata
     6601 ttagataata aaaatctaac ttattcagct ataggggtag tgacacaaat gttaagattt
     6661 caaaagtcag ggagccacaa aatatatgca aaatcattaa ttagttatag aaaagatagt
     6721 aaatcaagga taagcagtgc tttaaatgag cttataaatg aaggatttgt aataagaagt
     6781 aaaataagaa atgataaggg tcaaataaaa ggatatagat atgatatatt tgatacccct
     6841 caaattaaaa tagaagaaaa agaagatgtt aaaagtcaac cgtgtttcaa tataccgaat
     6901 acggttaatc ccgaaatcgg aaaaccagga agcgaaaaaa ccggcgacag taaaactgaa
     6961 atccagtttt tcggcaacat aaaagaaaat agtataaaag aaaataataa tactattatt
     7021 actattaata aggagcaaga agaaagcgca tatataaaaa aatattatga aacttatata
     7081 ggtttaatta cacctaataa ttttatagag ctagcaggtt atttagatga tggaatggag
     7141 gttgatgtaa ttataagagc tattgatgaa gcaacatcaa atggagttaa aaattataga
     7201 tacataaaga ctatattaaa taactggata gaatccggtg tgaagacaaa tttagattta
     7261 acagagtatc agaatgaata tgaaaagaaa aaaagaaaaa gtaatagttt gggaggagta
     7321 aaaaatgaca aacaaggaaa aagaaattct caaacaaaga gtacagaatt tgattttaac
     7381 agtaaagaaa caagaatcaa tgacaacgaa cttgaaagag caagacaggc acttagagac
     7441 attggagttg aaccaagata ctgaatataa atgtttaaaa tgtaaggata gacttttcat
     7501 agaaaccgat gaaggtttta aagaatgtaa atgtaggaaa cttaggatag cagaaaatat
     7561 tttaaaaaat agtggagtaa gtgaagaatt taggaaatta aagtttgata attttaatta
     7621 ccaagtggat tcttcagtag caaaagcatt tactaaagct attgagtact caaattattt
     7681 tgaacaaata agagagatta gaagaaattc aattatattt atgggacaag taggaagtgg
     7741 taaaactcat ttatcactag caatagctaa tagtctaatg aataggtcta taagtgttat
     7801 ttatatgcct tatagagatt ctatgacatt aataaagcaa aatataatgg atgaagaaac
     7861 ttatagaaga gaagttggaa agtataaatc agctaaggtt ttattaattg atgatttata
     7921 taaaggcaat ataacaaata gtgacataaa tataatgttt gaaataatta attatagata
     7981 tttaaataaa atgccattga ttgtatcaac agaaaaaaat tttgatgagc ttctcgaaat
     8041 agatgaagct attggaagta gattaattga aatgagtaaa aattatttga ttgaattaaa
     8101 gggcaaaaga ttgaattata gaatttatgg gggataaaaa tatggataaa aataaaatgc
     8161 tgaaaacgtt agttagaaag gctcaaagtg gagatgaatt agcttttaat gatatactaa
     8221 atgttcttga taaagagata aaaattaatg catctattta tgcaaaatat ttagaaggta
     8281 tttttgaaaa ggatgattta atagcagaag cacaagcagg attatggaaa tctataatga
     8341 attataaaat tgaaatagaa tatgtgagtg cttattttca aaaagttata aaaaataaca
     8401 tacttagatt agtaaataaa agtaaaacaa aaaagtttac aaatgagatt ttagttttaa
     8461 ataaaaatgt agattctaat gaatatacag aacttataga gtttatacaa gatgaaggct
     8521 tattggtaga tgaagttata attgagaagg aaagagcaga agaaatagaa gctgagaaaa
     8581 aattacaaat gaaaatagtg aaaagaaaaa ttagatatat tcctcagcca caaaaggaaa
     8641 ttataagaat gtatctaaaa gaatatgaca cagaaaaaat atcaaaaaaa ataaataaat
     8701 cttataacgc aatatatagt agtaaatata gagcagtaca aaatcttaaa gaaatgtgtt
     8761 taccagaaat aagtaagtat aatataacta tataatacct agtataaaaa taaaatagga
     8821 tatacaaata ttgtatatcc tattaaaaaa tgattatttt ggaggaatta atatgataac
     8881 tgcgtttaaa acaaaagaag aaagagaaaa agaagtattg gaaaaagcat taaattcttt
     8941 ttctggaatt caaaaggata gagcaagaat ttatcttact gagaataagg ttaatgatag
     9001 attattttta agtaatttat tagtgaaata caagtttgaa ataagagata acttaaagga
     9061 tatggaaaga ataagagatg aaatacgaga atataaatcg tatttaaagc aagagcttgg
     9121 taataagact aagataaaag agtatataaa ctatttagaa ggtttacttg atagttgtga
     9181 atctcttata tgtgcctatg gaaatgcttt aataccatta tttgatagta taaataaaga
     9241 atttactaaa aaagaaatat gtcaattgat tggagctaat gaaaagatat tagatgaata
     9301 ctatgaattt atgaaaaaac attctaaaga agaaatgagc aaagatttta attttatata
     9361 tgaattaatc tttgttcatc atggagaata tagatggaga aaaggaagat ataaagattt
     9421 tgttgattgt ccagattggg aaatgccact atttaatgct ttatggagtc atatggataa
     9481 ttttatagaa aataataaag aaatacaaga aagtatgcat tctgtatttg atgaagtatt
     9541 tggagatata tcttacttta tgaatggtga tggtacaatg gaaaaatcag tacaagaaat
     9601 aaatataaaa gaattaataa gtaattatag agattatgga aatagcactg tagtatttca
     9661 gttgaagaaa aaatcaatta tagatataac aataaattac aagctcaaaa gacaaagagg
     9721 tgatgtttta atctataaag tctttgatag tgaagataat gaacttggag aggtacataa
     9781 gattatataa gcttaatatg aaatttaaag ataaataaaa ggagctgttt aagtggataa
     9841 agaaagtata aaagaaattg taagagaatt gagaaaagaa gaaagagaag atagaaaaaa
     9901 aggagtctta tataatacta gattattaat gaaacattat aatgatttta aaagacatat
     9961 aaacttagca ttaagtgaag ctaaagaagt aaattattta gaagaagatt tgtctgattt
    10021 tgaagatgaa gagttgtata ttttaagtat taaaaaaagt aaaacaagaa ctattattat
    10081 gatagctcat atagattcag cattaaaaac attaaagtta agacaagaga gattacaatc
    10141 atatgaaaaa tatagagcat tagagcttta ctattttgat gaaaaaactt atgaagaaat
    10201 agcagaaata ttaaactgtg gagtagttac atcaagacgt tgggttaatg agatgataac
    10261 agagcttggt atatatttat ttggtgtaga tggtctaaaa tcaattgttt gataaatata
    10321 tgataaaaag atgatatttt aatgatattt taatcctgtt ataatggtag tatgaaacaa
    10381 gtgtataata tactccttaa aaaagactaa gtacccctaa cttagtcttt tttatattta
    10441 caacaaagga gaataaaaaa tgaacatgat taatttaaga gaaaagataa taaaagaact
    10501 aaatgaattt aatataaaag cagataatgt ttttttagaa tatgcagtta aacatattga
    10561 aaattttatg aatgaaggta tagatgatga agagttatta ataagaggtg ttgtgcttgg
    10621 agctagttat gtagttaata tagaaaataa agacttagat taatttctag gtcttttaca
    10681 ttttataggt taagtttgtg gtaaaatgta ctaaatgaat taggggtgat aactatgaag
    10741 aaaaatatct taggagtatt gctaccatta ttaacaatga ttataatatt ttttctatca
    10801 tttgaaaata caataactaa taatttagga ttaagtgaga tggatataaa aggaatcctt
    10861 atttcaggtg tagtattgta tataccagtt tcatttttag ttaatggtat aatatgtgct
    10921 aaaaaacaaa taaattggaa aattccagct ttaatatcac ttttaggttc agttgttata
    10981 atgatgtatc taagatttga tagtactgaa atggcattgt atttatcata ttattctatg
    11041 tcttattgta ttggatattg gataactagt gtaatgaaga aataaaatta aaagattact
    11101 tttatatatt atggtaatat gaaagaaagt aattattagg gggtataaat atgaaatgtc
    11161 ctaattgtgg tagcgaaaat atagaacaag gtatttctac agttagtgaa tcaggattcg
    11221 ttatatatag aactggttta gaatacaata caagattttc cactaaggta tctaaaacat
    11281 attctgactt atgcttagat tgtggtgaaa tagttagaac ttatataaag ggtgagactg
    11341 acaggaattg gtgtaaagat tagaaataaa aatacataag atttagacta tctttataga
    11401 tggtcttttt tatgcaataa attaaaaaga taatgataaa tatgttatag gttaaggttt
    11461 tgaacttgtg aaacagagta acagaactta ataggaatta gctttattct ttattatatg
    11521 gtataatgta aaatagagat aaggaagtgg ttagtatagg aatattaaaa agtgctgaat
    11581 tagttagaaa gatagcacaa agaattgcta gagaaaaagg aataagagag caagaagcat
    11641 gaaatgatgc tgttacagag tacaagcaaa aacatatata cttagtctag agaatcatct
    11701 ttatagatgg tcttttttta tacaataaat taaagaagag cattatttag aattattttg
    11761 tagttgtcga acgattgttg aaggatattg acctttaaag ttgaagctta tactttggag
    11821 gggataatgc agaataaatt aatgagagtg aataatagat aggtgtattt aggagggata
    11881 aaaattgagc attaaagtta aagtatgggc taaattacca tatattataa aactagaaga
    11941 agagaaagat tatgaattta gttttctatt tgatgatttt gatttattta agcttaatat
    12001 gaggtttaat gatgaaaatg attttttaat ttataataat gaatatcctg gtaatgtgtg
    12061 tcagcatata gaattagaag taatttataa atatgttgat tatacaaaat ataaaactac
    12121 aaaatgtatt aatagtaatg acaattattt tgaagtagag gtgccattag ataaaactac
    12181 agagattttt ggatgcatga ataaaagact taatgaaata ttaaattttt taagagagaa
    12241 gtctaatatg ttttggattg atgaatttaa tcttacacat ataaattatc attctgagaa
    12301 tgaggtacgt tttaactttt attctccgaa tgtaaatcca agttctgttg taactagttt
    12361 tatagaacat acagattact atatgatttc taaaaacttc aaaacaaata atgttggtga
    12421 agataccttt aaaaactttg attctaataa gataaaaatg aaggttttta atgaatatat
    12481 agacaaagca gagaaagcac tgtatgaata taattatgaa aattttataa tatattgtag
    12541 tatatcagtt gaatcattta ttattcaata tatagatgaa atagctaatc aagacgatat
    12601 tatttataaa caacttgaaa aaacaaactc taattatata aatttgtatt ataatgtgtt
    12661 attaaaatat ctaacaggca aaagccttat agaagttgat aaaaaagcat gtacacattt
    12721 aacaagactt tatagattaa gaaattcatt aatgcataag ggaattatag atagtaaatc
    12781 attagaaaaa gcaggtataa tgaaacttga ttttaatgag tgtaataaaa ttttagattc
    12841 agcaaagaca agttttaaat tagtaaagag tataacagtt aaacctaaga atgatattaa
    12901 ttaataaaaa taataattac tggaaaagaa ctcaataaat agggttcttt ttttattccc
    12961 aaaacgacaa aaacgaggtg gtggtgtggc aaaatatgaa tactggataa cagaagaagg
    13021 attaattaag attgaaggat gggcaagaga tgggcttaca gatgaacaaa tagcatttaa
    13081 tattggaata aatgtcaaaa cactatatga ctggaaaaag aagtatagta atatttgtaa
    13141 tgccttaaaa aagggaaaag aagtaattga caggcaggtt gaaaatgccc tattaaaaag
    13201 agcattaggt tatgaatatg atgagataac atatgaagaa ggtcaagaaa ctaaaagagt
    13261 aactaaacaa gtagtaccag atactacagc acagatattc tggttgaaaa atagaaaacc
    13321 agcggaatgg agggataaaa gagatattga acatagtggt aatctaggag atattacaat
    13381 aaaggtaggc gatgaggaat atggcaatta atttagaaat taatccagat gtatttaatc
    13441 caatatattt gaagcatcaa cttaataata acaataggta tcagatttac tttggtggtt
    13501 catcctcggg taaatctttt tctttagctc aaagaacagt attagatgta tttaagggga
    13561 ataggaatta tttaattgtt agaaatgttc aaagcactct aaagaggtcc tgtttaaatg
    13621 agataacaaa ggctattaat aattttaagt taaatgagta ttttcaagta aataaaacca
    13681 atatgataat aacttgtaag ctaaataata aacagatatt attttgtggc ttagatgatg
    13741 ttgagaaagt taaatcaata actccaatag atggtgtaat aactgatata tgggtagaag
    13801 aagcaacaga gacagattat aaagccgtca aacaacttga taagagactt agaggaaagt
    13861 ctaaagtagt aaaaagacta acattaagct tcaatccaat acttaaagac cattggctat
    13921 atacagagta ttttgatata tgggaagatg ataaacagta tgtagaaaaa gataatgtaa
    13981 gtattctaaa aactacatat aaagataata aattcttggc agaagatgat ataaaagctt
    14041 tagaaaatga aagtgataaa tattattatg aagtttacac tcttggaaat tggggtgtac
    14101 ttggtgctgt catatttaag aattggagag ttgaggattt ctcagatatt gaaagtacat
    14161 ttgataactt taggcatgga attgattggg gatttgctga tgacccattt gcatatgtaa
    14221 aatcacacta tgataggatg agaagaaaat tatatatatg tgatgaaata gaagcagttg
    14281 gattattaaa tagagaagct gcacctttgg ttagtaaaaa agcgaatgga gatttagtta
    14341 tctgtgataa tgcaagtcca aaagatgtag ctgaatttag tgacttaaga atcaatgcag
    14401 tttcagcaag aaaaggagct ggttctattg aatatggtat taaattttta caagggcttg
    14461 aaattataat acatccaaga tgccaaaact tcaaaaatga gataaataaa tataaatata
    14521 aagaggataa gaatggaaat atcttaccta ttgcagtaga taaagataac catttaatag
    14581 atgcactgag atatagctta gaaaatgata tggagtatgg aggaataagc ttcttaaagt
    14641 aaggaggtgt taaatattta tataagtgaa acagatttaa taaaagctca gctaaaaaaa
    14701 gagagtactt ttaatctggc gaaagttata gagcattata ttttaaagca tagaccagaa
    14761 aagtatcaag aaggagaagc atactattat ggtaatgcta atataaaaaa taaaagaaga
    14821 tattatattt tagatggtgc taaggttgat gattttacta aagttaataa taaagcagtt
    14881 aataattatc ataagctttt agttgaccaa aaggtaggct acagtgttgg aaaaccaata
    14941 gtatttaatg cagatgataa taattttact aagcttttaa atgaattatt aggagaagaa
    15001 tttgatgata caataacaga actctatctc aatgcaagca ataagggaat agaatggtta
    15061 catccatata ttaatagaaa aggagagttt aaatatgtaa taataccagc agaagaagct
    15121 attcctattt gggataacaa gaggcaaaat gaagtagttg cctttattag attttacttt
    15181 attgaggata tagatggaaa taagataaaa agagttgaat actatacaga aaatgaagta
    15241 atttacttta ttgaaaaagg aaatagtttt gttcaagaac ttttatatga tgaatatgga
    15301 aaattaacag agatacaaga gggtcatttt agagtaaata acaaagagca aggatggggt
    15361 aaagttccat ttataccatt taaaaacaat gagaaaagcg tatctgattt aactttttac
    15421 aagtctttaa ttgatatata tgataataac atttctactc ttgcagataa cctagatgaa
    15481 gtacaagaag ctatgtacat actaaaagag tatggaggta ctgatttagt agaatttgca
    15541 aataacataa gatattataa agccattaaa gtaggagcta atggaggggt agataagctg
    15601 gagataaata taccagttga agctaaaaag gagcttctta atagattgga aaagaatata
    15661 attatctttg gtcaaggagt taatccagaa tctcaaaaca caggtgacaa atcgggtgta
    15721 gcacttaaat ttttatattc actacttgac ttaaaatgtt ctaagactga aaagaagttt
    15781 aaaaaagcaa ttagagagct tttatggttt gtgtgtgagt atttaaagat aagtggtagt
    15841 aagagctatg attataaaac agttcaaatt acttttaatc actctatgat aataaatgaa
    15901 gctgaaaaga tagatatggc agctaaatca actggaattg tatcagatga aactattgtt
    15961 tccaatcatc cttgggttga ggatgttaat gacgaacttg agagacttaa aaaacaggaa
    16021 gatactcaaa aagagtatga tgatttaatt cctaataatc aagatggtgt tatagatgaa
    16081 atataaagat tattggagaa agagatttga acaattagaa gaagttcaaa ataacaaaag
    16141 tgtaaaatat tatcttgaat tagaaaagca atataaacta gctatgaata gtatagaaaa
    16201 agatatatta tcatggtaca acagatttac tgaaagtgaa ggcatatctt tattggaagc
    16261 taagaaacta ctaaatacaa gagaactaga agagtttaaa tggagcgttg aagaatatat
    16321 taaatatggt aaagaaaatg ctataaatca aaagtggatg aaagagttag aaaatgctag
    16381 tgcaagagtt catataacaa gacttgaagc tttaaagtta caaatacagc aacaagtaga
    16441 agttttatat ggaaatgaac ttgatggtat tgataaacta atgagagata tttatacaag
    16501 tggatattat catacagctt ttaatgttca acaaggagta aacgttggtt ggagtttaat
    16561 gagtcttgat actaatagaa taaataaaat tatctctaaa ccatgggcaa cagatggatt
    16621 aaactttagt gaaagaattt ggggtaagca tagacttgct ttagtaaatg cattacatac
    16681 taagctaact caatcaatta ttagaggtga aaatccaaag aacctagtaa atgacttttc
    16741 taagaggttt aatgtatcta aatcacaagc taagaattta ataatgactg aatcagcttt
    16801 ctttgcatca gcaagtagaa aagattgttt tagtgattta gatgtagaga aatatgagat
    16861 tattgctaca ttagatttaa gaacttcaaa tatatgcaga gagttagatg gaaaagtatt
    16921 tgatatgaaa gattatcaag ttggaataac agctccacca tttcattgtc gttgtaggac
    16981 aacaacagct ccttttttta gtgatgaaga aggctataga gcagcaagag gagaagatgg
    17041 aaaaacatat tatgtaccat ctagtatgaa gtataatgag tggtatgaga agtatgttaa
    17101 acataatagt atcttagaaa taaaaaatag tgctataata gatagcataa aagaagatat
    17161 taaaaatggt aaatataatt taaatattca tgatgggaaa caaggaaaac atttaaaaga
    17221 gcataataat tatatagaag gaagaagcta tttaactata acaaaagaag aagctcaaga
    17281 acttgtgaat aagcatgctg gcaatggaat cataaaattt aatcgaagtg gagaatggga
    17341 taaaaaagaa cttatagaag ttgataagaa tataggggtc aatgttaata atattacggg
    17401 tgaaaaaact cttacaaata agtttaagat acattattcc aaaactggaa cacatatagt
    17461 accagcttta taaggaggaa aaataaaatg aaattatggg aatatgtagg gaaaaatgtt
    17521 caaataactt gtgtagataa gcaaataata agaggaaagt gtgatggata tacacaagct
    17581 ttagataatg aaccagaaat agcaagtata tcaatagcta gggatggtta tggaattgaa
    17641 gtttatgaaa atgaaataga atctatcgaa aatataaata aggaatgact aagcatgtac
    17701 ttaaaaataa gtagatgctt ttattttgta aaaaatgaaa ggagaaattt aaaatggatt
    17761 ggttaaaaga attgctagaa ggaataaaag tagaggataa caaaattgat gtagcttctc
    17821 ttcaaaaatc tatagaaaag aaaataaagg agactacagt tactcaagaa gattatgcaa
    17881 atattgaaac acaacttaat acagctaatg aagctattaa aaagtttgaa ggaggtatga
    17941 caaaagaaga tgtagagaat ctaaaaacaa cttatgaaac tgataagaaa actttggaag
    18001 aaacctacaa aaaagaaatt gaagaaaaag attttaatta ctggttaggt gatgctttta
    18061 aatctgttaa atgtagagat gaaatagcat taaaagctca tttagatata gaagcactaa
    18121 gaaatagtaa agatagacaa aaagcttttg aagagcaaat aaaccctttg aaacaggata
    18181 aagattattt gtttaatgca acactagaag gtgaagagcc taaaatagat actataacac
    18241 cagggcaaga gcctaagata aatgattttg gttttaattt tactggggta agacctcatg
    18301 aaaataataa taaataggag gaaataaaat ggcagcacta aattatgcaa aagaatattc
    18361 aaatgtttta gcacaagcat atccttatac tttaaacttc ggggatttgt atgcaacacc
    18421 aaataatgga agatatagat ggactggttc taaaacaata gaaataccaa ctatatctac
    18481 aactggaaga gtagattcaa acagagatac aatagcagta gctcaaagaa actatgataa
    18541 tgcttgggaa cctaaggtat taactaatca aaggaaatgg tcaacattgg ttcatccagc
    18601 agatataaac caaactaatt atgtggcttc aataggcaat ataacaaaag tatataatga
    18661 ggaacaaaag tttccagaga tggatgctta ctgtatatct aaaatatatg ctgattggac
    18721 cgcattaggt aatacagcag atacaactgt tcttacaaca acaaacgtat tagaagtatt
    18781 tgataagtta atggaaaaaa tgacagaagc tagagtacct gaaaatggaa gaatattgta
    18841 tgttactcca gtagtaaata cacttatcaa aaatgcaaaa gagatacaaa gaacagtaaa
    18901 tataaaggat gcaggaactt ctcttaatcg tcaaacaact gatattgaca cagttaaaat
    18961 aattaaagta ccatctaatc taatgaaaac tgcatatgat tttacaactg gatggaaagt
    19021 aggagcagga gctaaacaaa tctttatgtc cttagttcac ccaagtgcaa taattacacc
    19081 tgtttcttat cagttctcta agttagacga accaacagca gttacagagg gaaaatactt
    19141 ctactttgaa gaaagttttg aggatgtatt tatattaaat aaaaaagctg atgcaataca
    19201 atttgttgtt gaaggagctg gagcataatg gcacaagtaa ggaaattaaa tagaatatta
    19261 actatagaag aatgtaaaat agatgatttc ttagagatgg gatatgattt gatagatgaa
    19321 actggtaagg cagtaaagta tggcaagtca ttaaatgtaa aagatttaat agctgaaaat
    19381 aatattttaa ggtcaaaagt tgagtcttta gaagaagaaa ataagcagct taaagagaaa
    19441 aataaactta ctaaaaagta ggtgaaaatt atggaaaata atataattga tgaaatagaa
    19501 aaaagacttg aaagttttgg atatatatta aaagatggag ataagtggtt aataggtttt
    19561 gtaagagaaa aaatagaaaa tattattaaa ctagattgta atataaaaac tatgccaatt
    19621 gaattgaaag aaattgaagt tgatatgata gttggagagt tcttatttac caagaaaaat
    19681 atgggtcaat tagatataga aagcattaac tttgaagctg tagaaaagtc tatatcagaa
    19741 ggtgatacaa aggtagattt tgctatagga agtggttctc aaacaccaga acaacgcttt
    19801 gatagcttaa tagcttatct tactacttat ggtaagaata agatattaac ctttaggtgc
    19861 ttaagatggt aagtaaaact agaaaagcaa tagaaatgtt atatagagat aaatgtacta
    19921 tagttgagta tcagccaatt aaagaccctg taacaaaacg aactaacaat aaagaagtga
    19981 ttgtattaga aaatcaacca tgtaaacttt catataaaaa tatagtttct gctacagaag
    20041 ggaaattagc taagctagag caaactatta aactctttat atctccagat atagaaatta
    20101 aagcaggttc aaaacttatt ataaatgata aagagtatgt aagaagtgga gaatcagcta
    20161 tatatccaaa tcatcaagaa ataatacttg agctatttaa ggataaagca taatggctag
    20221 atggggcagt gttgatttta gagagtttaa aagagtttgt aaaaagatgg agaagcttac
    20281 aaagattgat ttagataagt tttgcaagga tgcagcaaga gaattagcag cacgattact
    20341 tgggaaagta attagaagaa caccagttga tacaggattc ttaagacaag gatggaatgg
    20401 agtggcttac gctaggtcgc ttcctgtgta taaacaagga aataattata ttatagaggt
    20461 tgttaatccg actgaatatg ccttaacaca atggggcata taaaacccag caaaatcggt
    20521 aaacgctaag tataatagtg ttacattata aagtaggtag aatatggaca tagaataact
    20581 cttgatttat aatgtataaa agaggtgatt ttatggcaaa atttttagat attacaggaa
    20641 aaaaatttgg aaggctaaga gtcattaaat tttctaaaga gataaaaagt ggtaaaagaa
    20701 atagaaaata ttggttatgt aagtgtgatt gtggaaattt caaagaaata agaactgact
    20761 ctttaactag tggcttagta caatcttgtg gatgtttaaa aaaagaacaa gataaattaa
    20821 atttaacaga caaataccaa tttaaaaaga aatataaggt tcaaaataaa agactttaca
    20881 gtatatggaa gggtataata tctagatgta cagataaaaa taataaaaga tacaatagat
    20941 atggtgaaag gaatataatt gtgtgtgatg aatggttttg ctatgataat tttgcaaatt
    21001 gggcattgag taatggatat tcagagaaac ttacaattga tagaataaat aatgaaggaa
    21061 attatgaatc aagcaattgt agatgggtgg atataaagac acaatgtaga aataggtcaa
    21121 caaatatatt agtaaagcat gaagaaaaag aaattacatt gatagagctt tctgaaaaaa
    21181 caggtatttc atattcttgc ttaagaagta gatattctaa gggattagta ggaaataagc
    21241 ttatagaaaa agttaagatt attgaagaaa gcagagctaa gttatctatt gaggatgtaa
    21301 aagaaattag aaaaaagtat tctgatggat atacaataaa acaactaagt gaaatatatc
    21361 ctgtaacata ttcatctata tcaaatatag tccatagaag aacatggaaa aatatttaat
    21421 taattatata tgccaatacc gaggaaaacc tatagattgc gaataggcta taggtttccg
    21481 tagagcgtag agagtgaata aatataatct ctccaagagt gctgggcaac taaataaagt
    21541 ttattttgtt gatgatgtac gccgaactta taggaaacta taagagctag aggataaaaa
    21601 gcctttagga taacaaaatg catatgttga gtttgggcat agaactaaaa gtggtaaggg
    21661 atgggttaaa ggacaacatt tcttaacaat ttcagagatg gaactacaaa gccaaattga
    21721 taagattata gagaaaaagt tattaatatt acttaaagga gtatttgatg cttaataata
    21781 taattgatgg aatatctatt aaattagata aaacatttgg agagagttat acaatttata
    21841 gtgaagatgt ggagcaaggt ataaatgaac cttgtttttt tatttgccct ctaaatccaa
    21901 gtaagacatc ttatccaaat ggtagaacac taaaaaagaa ctcttttgat gtacattatt
    21961 ttcctcgttc aaaagataag agttttgaaa taaatgaggt agctgagatg ctacttgagg
    22021 aattagagta tatagaaatt gatggagatt tagttagagg cacaaatatg aattttgaaa
    22081 ttatagataa tgttcttcat ttcttcgttg attataacta cttcactata aaaagtaatg
    22141 atacagaaaa aatgaatgat gttgaattat ttggtggttt gaagagaggt gataattttg
    22201 aataaaacat taagcaaaga agatgactac aagtttacta aggagcaaat agttaattct
    22261 aagaagtatg taaatagaaa agacttatta aatgcaattt taaaagaaaa tgagttatat
    22321 tccttctcag aagtagagga aataataaat aattttatga aaggagtgag ttagatggct
    22381 ttaggtggag gaacatttgt aacacaaaat aaggtcctac ctggtgcata tataaatttt
    22441 gtaagtgcta caagggcaac cagttcatta tcggatagag gtattgttgc aatgccttta
    22501 gagttagatt ggggcataga tgaagaaatt ttcacagtaa ccagtgatga ttttgagaag
    22561 tattcaacta agtattttgg atatgattat actcatgaga agctgaaagg tttgagagat
    22621 ttattcaaaa atataaggtt gggatatttt tataaattga ataaaggtgt taaagctagt
    22681 tgcagtattg ctacagctaa gtactcaggt actagaggta atgatttaaa agttatagtt
    22741 acaacaaaca ttgatgataa cactaagttt gatgttgtaa cacttttgga taataagaaa
    22801 gtagataccc aaatagcaaa agttattaca gacttacagg acaatgacta tattacttgg
    22861 aagaaggata caacactaga agcaagtgca gggcttgtat ttactggtgg aactaatggc
    22921 gaatcagtca caggagcaga gtatcaagct ttcttggata aaatagaaag ctactcattt
    22981 aatgcactag gcagtttggc tacaacagca gaaattaaaa gtttatttgt agagtttact
    23041 aaaagaatga gagataaagt aggagctaag tttcaaactg tactatataa aaagaatgat
    23101 gcagattacg aaggtgtagt atctgtagaa aataaagtta aagatactgg gttattagaa
    23161 tctagtttag tttattggac tactggagct atagcaggat gcgatataaa taaatctaat
    23221 actaataaaa agtatgatgg tgagtttgat gttgatgtaa attacacaca aatacaactt
    23281 gaagaagcac taaagagtgg taaatttata tttcataaag ttggtgatga agttcatgtg
    23341 ttagaggaca taaatacttt tgtatcattt acagatgata aaaatgacga tttttcaagt
    23401 aaccaaagtg ttagagtact tgaccaaatt gctaatgata ttgcaacttt atttaatgaa
    23461 aagtatttag gtaaagttcc gaatgataag gcaggaagaa taagtttctg gaatgatgtt
    23521 gttaaacacc ataaagaatt agagaatata agggcaatag aagattttaa aactgatgat
    23581 gttagtgtag agcttggaaa tgataagaaa actgtcatag tatctgatgc tgttaaggtt
    23641 ataaatgcta tgagtaagct ttatatgact gtttcagtta gttaagagag gagtgtgata
    23701 tatgtttcag caaataaaag caagagatac aataagtgca tctaaggcag aatgctatgt
    23761 tactatcgag ggtaaaagat ataattttat gcaagctatt aacttagaag ctaagatgga
    23821 aaaaaataaa agtgaagttc ctatcttagg tagcactaca aaagggaata aaagtactgg
    23881 aagcaaatac acaggaagtg caacatttca ctacaatact tctatattta gagagcttct
    23941 ttataggtac aaagagactg gtgaagatat ttattttgat atacaggtaa caaatgaaga
    24001 cccaacgagt tcagctggac gtcaaactat aatacttaag gattgcaaca tggacagtgg
    24061 aattatagtt aaatttgatg cagatggtga gtatcttgat gaagatatgg actttacatg
    24121 cgaggattgg gaattggttg aaaagttcaa tataataaat ggaatggaat aaaacacaca
    24181 tttataaatt atagatgtgt ttttttatat aaaaaattaa aataaaagga gattaggata
    24241 atatgagtaa tttaagtgct tttttaagtc aaaatgcaat aaaggttgat aatgtaaaat
    24301 atatagcaag tgatagattt ttagacgaag aaggaaagcc agttgaatgg gaattaaaag
    24361 ttttatcttc tgaggaagat gaagtattaa gaagaaattg taccaaaaga gtaaaagtga
    24421 ttggcaataa cgggaagcca acaggtcaat tcacaagtga aattgattat aacagttatg
    24481 tagctgaatt atgtgtagca tctacagtat ttccagattt aaaggatgcc gaactccaaa
    24541 atagttatgg agtgatggga gaagctcagt tattaaagac aatgcttaca gcaggtgagt
    24601 atgtcaacta tacagtaaaa gttaatgagg ttaatggatt tgatacaaca tttgaagata
    24661 aagtagaaga agcaaaaaac taattagggg cggcgatttt gatgctagta tcacgcatta
    24721 ttgtatccaa aaattaaagt ggaaaccagg tgattatatg ggattagaga ttaatgagag
    24781 agcattagca gccgcctcaa tacttattaa gatagaggat gaagaggaag caatgaaaga
    24841 agctgaaaga gagagaaaga ggggtagaag aagatagcaa aataaaaaaa taaatataga
    24901 ataggtaaaa tatgtaataa ttatatgtta taatattttt agcaagaaga tgtaatctac
    24961 aatttataga gtggagttca tacaaaagat tatcctccca acgtatagaa gggaggtgaa
    25021 tatgtatgga taattttttg attggtgtat tagctagttt aacagctagt ctaatcggtt
    25081 acatagtttg tctatgtatc aaaaaagtaa aaagccactc tgtgcaagag agtgacttag
    25141 atgttgagct taaattttca tttaagttca aaaaaaataa gcattaaatt gtttagaact
    25201 tcactctact tccaaataga ttgtagttct tcttgttttt attataccac aaattggtac
    25261 agatattcaa aaataatata tttatgatat aataaaaatg tagagatttt gcagtgttcg
    25321 atttttgtaa taaaatatgg tttaacaatt ggaatacaag gcattgaggg tgtgtgataa
    25381 atgttatcaa ttgcactact catggttcac tgcaaatttg agagaggtgt gtatgtgtag
    25441 gtattggaaa tgctaagttt attttggggt tttagattaa ctatatggaa tgtaaataat
    25501 ttttcgagag tctcaattta cagaagaaat tggagtttta gattaactat atggaatgta
    25561 aatcaaattg tctaatccta attttttctt tgcatcttct gttttagatt aactatatgg
    25621 aatgtaaatt gtaattaagt tagatatggc aaaagaaata gctatgagtt ttagattaac
    25681 tatatggaat gtaaatttta atgattctac aactcttaca gactcagatg atgttttaga
    25741 ttaactatat ggaatgtaaa tgggtgggag atatggcaac tatccacaca tgaaattggt
    25801 tttagattaa ctatgtggat tcaaaattaa ataaacaaag aaagcactta caaacatgta
    25861 ggtgcttttg ttatgtaaaa aaatagaggt gattaaatga ataaagatat agaatttatt
    25921 gcctgttcaa tgaggttaaa aattttgata caagcaagag aagacttaat tgaaaatata
    25981 aataaatatt caactaagtc ttatgaaaaa aatattgata attataaaaa attagatatg
    26041 attttcgaag atgctataaa gtttgaagct atactcctat catctttaaa ataacactag
    26101 atgcaacact gcctataact tttaaagagg cggaagatgc aaaattacct aattgttttt
    26161 tagttttatt ccaaacagtg tcatctctta tattgtcaag ataatcataa ccaaaggagg
    26221 ttatcctctt aacaatataa tgtttatata agcatctttg aacacccatt ggtgtagctt
    26281 caataaagcc aacatccaat aataattgta aatgataaga aatagttctt tcatcataga
    26341 catcagtcat gaaatcactg atagacattc tatcagcatc agattcttcc attttgatta
    26401 gaatatctct tatcaattct aaatcacgtt tcattatatc accatccttt cataattaat
    26461 ttaggaagta tctactaata tttcctaaat taattatagc atggtatatt agggagattt
    26521 gtttatatta agaataatat gtggatattt tatgtatgta gtatatgaac taaatagata
    26581 atatagtaag cacttacaaa tatgtaggtg cttttataaa atatttgaaa tgatagcaca
    26641 ttcccgtttt gggaacatgg tcaaaactca aactatatta ctagtatatt aaatatgtag
    26701 tatcaaaatt aaataaagaa aaggaagcac ttacttttgg taggtgcttt tgttttgctc
    26761 aaatttggtc ggttgggtaa aataattaga aaaaaagata tagctgaggt agtgttttac
    26821 gacgctagct tcaataaaat tttatttgca aaatattcca aaatagcttg actgtaactc
    26881 gttacaatgt tattattaat gtaacgagtt acagaaaaga ggtgaataaa atagcaacta
    26941 aaagtagagc agagtatatg aaaaatcgtc gaaaagataa aagaggtttt agtgtacttt
    27001 tagacaaaga aaagttagat aaatttgatg aagtgttaga agagaagaat ctaactaaga
    27061 aagaatggct agaagaaaaa atcgacgagg aactggaaca aaaggaataa aaaataaggg
    27121 tcactcccac cgaccaaagt ttgagcaacc cttattgacg tatactatac atcaactaac
    27181 tatagtatac gtcattcctt aaaaaatttc aattaaggag tgtaatagtt atgaaaaatt
    27241 taatagtaaa agagttcaat ggaagtcaaa tttatacttt tatgtggaaa gaaaaatctt
    27301 gttggatagc taatcaaata gttggattat tcgattatgc tgatgtatct aaaacaatac
    27361 aggattgcat aaaagcagaa gactttgaga ttgaacaaga gtatgatgta ttgaaaggaa
    27421 atgaatttaa tgattttgta actactttaa atgtagtcgc aaataatata attagtaata
    27481 aagctagaag tataactatt ttttatgaag atggtttata tggattttta caatacacag
    27541 acaaacctat tggtgtacag tttaggaaat ggcttagacg agaagtttta ccagctattc
    27601 gacaacatgg tgcatacata acaaataacg ctgaccctca agcattaaga gaaaaagcaa
    27661 atgaaataga aagtttagat acagttaata agactataga aatactaact ccattcttag
    27721 ataatgctgg aatagatgaa aaagcaaaat tacttacagc aaagactatc tacaaaaagg
    27781 caggaataga gttacctctt gaaatagaag agaaggaaca tttctttgat actgtacaaa
    27841 tagcaactaa gttaaatgtt tattctaagt ctaataaacc tgcatttcat gcaataggtg
    27901 aaattattaa gaagttagat atacaagata atgaaaagtt agtagtacta gagagtaaaa
    27961 gaggttggag tggaagtgtc aataagtatt cacagagtgt aatagataaa ataagacatt
    28021 ggatagagga gaataataga cctactaaga tacaaggtga gaagaagaat tttcatgtgg
    28081 tttataaaat tgagtaaatt tatcagttgt attaaataat atattttagt ttattttggg
    28141 gggttaatac aatgtgtgag aatttacttg atatggatag aatagaactt attagagaac
    28201 ttggaagtat ctttgaaaaa atgagaaatg aaaatccaga taaattttat agatttgtaa
    28261 gtttagtgaa agaagaatgt aggaaaaaag aagagaaaaa taagtaaata atataaataa
    28321 agcacttgga tattatgttg tttcaagtgc tttgtttgtt aaaaagtggt ataataaaaa
    28381 tatagaaatc tataaacaca agtcgtttta tattaactaa gtggtatgta aatcgtacag
    28441 atagtattgc attagaggaa agtttaaaag cgttttagat taactatatg gaatgtaaat
    28501 gtttttaaag cttcttcaag ttgtatttgt gtataatgtt ttagattaac tatatggaat
    28561 gtaaatccaa ctgaataaac aataatttta tcaatttctt tagttttaga ttaactatat
    28621 ggaatgtaaa ttattttaca gatgaacaat tacaattact tcttgaatgt tttatattaa
    28681 ctaagtggta tgtaaataaa gcaaaagata ggaatgataa ttttaaaact ccaaactttt
    28741 atattaacaa tgtggagaaa aactaaatag aagaaagaag cacttatttt tggtaggtgc
    28801 ttttattttg ctcaaaatat tttaaatcaa tagactaagt tcttattttt agatagaatt
    28861 atatttgaat aaagaattta ataggggaga gttcattatg tggggaaaat ttaaaaaatt
    28921 aagtttgttg aagaaaattt tagtgatatt tttaatatac tttgttgtat ttacagtatc
    28981 aatgatgatt catcaagcga tacgagattc aaaaaataga gatgaagtaa atgaaggaaa
    29041 tattacaaaa gaaaatataa ttagcgaaag agaaaaagaa gatatttata aacaagagat
    29101 gcaagcaaaa gtagactcta tgataccaga agacttaaaa gataaaacaa catattatgt
    29161 taacatatta aacccaacaa aaggtgaggg atatatagtt agtattcaag tggaaaattc
    29221 taggtttaat gatgaaaatg agtgtagaaa ctttactaaa gaatttgtaa acaatataaa
    29281 agatatgaat gatatccatt cagtgagaat aagctttatt gttgatgtga cactcactta
    29341 taatgtattt ttagatgatt ggaataatat aaaaaataat gtaaatttaa ttgatgattt
    29401 ggatttctcg tctggaaatt aggtgaatac tttattttaa aattaaatct gttatataga
    29461 aagcacttac tttttggtag gtgcttttgt tttgctcaaa attggtcggt tgagtaaaat
    29521 aattagaaaa aattagtaaa aactcttgaa aagtgtcgcg atacaatgta taattatatt
    29581 atcgcgatac agaaaagagg tgaaaattat tactgatagc agtagagcag attacttcaa
    29641 gcagagacga cagaataaga aaacttttag tgttctacta gatagagaga aagtagaaaa
    29701 aattgaagaa catttaaaaa agcagaacaa gactaaaact atttggcttg aagaaaagat
    29761 taatgaagag ttagaaaaag aggaataaaa aataagagac gttctccccg accaaagatt
    29821 gaacatccct tattgacgta tattatatac actaactata gtatacgtca ttccttaaaa
    29881 aaattcaatt aaggagtgta atattatgaa aaatgaatta atgatgtttg aagaaaagaa
    29941 agttgaagta cttgaatata atgggcaagt tttatttaat ccatatgatt gtggaagatg
    30001 tttagagtta agtgatagtg caataagaaa tcatttatct aaaatgaatg atactcaagc
    30061 tgtattatta aaaaattcta atgtcctaga taaggacttt agaaaattgc ataacacagg
    30121 tgaaaagttc ttaacagaga gtggagtata taagttaata tttaaatcta aaaaagaaga
    30181 agctgagaga tttcaagatt ggataagtga tgaagtactt ccagccattc gacaaactgg
    30241 tgcatacata acaaataatg ctgacccaga taagttgaga gaaaaagcaa gcgagattga
    30301 aaaattacag ttagcttaca atagtacatc tatgttaaaa gaactattag atggtgcagg
    30361 ctttgacaat aaatccaaac tattaacagc taaaacatta tataagaaag caggaattga
    30421 tttaccaata gagataaacg aagaagaaca ttattttgat acaaagcaaa tagcatctaa
    30481 actgaaaata tattctaaga gtaataaacc agctcagatg gctgtttgtg aaattattaa
    30541 aaagattgat ttagaagaaa acgaagtcaa aggcgtttgg gaaactaatg gttcttggac
    30601 tggtactgta aataaatata caaagagtgt aatagataag gttagaaatt ggatagagga
    30661 aaataataga cctgctaaga ttgcaggtga aaagaagaat tatcatgtgg tttataagat
    30721 tgagtaaatt tatcagttgt attaaataat ttagtttagt ttattttagt tttgaggggg
    30781 attaatacaa tgtgtgagaa tttacttaat gaatataatt taaaaactga tgaagatgta
    30841 gaatactttg taaagtttgc tacattatta tataaattaa aacaggacaa tgaagaaaaa
    30901 tttcaagagt atgcagagat attgagaggt attcttaggg aacaacaaga gagagaaaat
    30961 aagtaaaata atatggataa agcacttgga tattatgttg tttcaagtgc tttatttggt
    31021 aaaaaatggt ataatatagg taggaaatta tattaactaa gtggtatgta aagggagtag
    31081 gcaagtttta taaacaaata acaggaagat tttatattaa ctatgtggta tgtaaatgcg
    31141 tttaggaata tgacagcatc tattaatacg acaagtttta tattaactat gtggacttaa
    31201 aattaaaaat aattcaaaaa cacttacaaa tgagtaagtg tttttttaat gaaaggaggt
    31261 gataatgatg taaaaatttt actgatatag tataataatc ctataaattc attataataa
    31321 agggggatat tatgggatta tttagtggaa atgaaagttg ttgtatatgt ggagaaaaag
    31381 gtaaacaaaa aatatctgat ggtgtaatat gttctgaatg tttaaaaaaa tataaagata
    31441 cattttctat agtcgaacca acagatgtaa ttaaaaaaat atcttctgag gaaataaaaa
    31501 aatcaataag attaacactt gaaaataaaa aaagatttga atcttttaac gcaagtaaaa
    31561 aagtaggaat ttatttatta gtagatgaaa ataaaaaaca attaattata tctgataaaa
    31621 taagcaattt aaataagaat aagagagtat atgactttag ggatattatt tcatttgaac
    31681 ttttagaaga tgatgaatct ataattaaaa gtggattagg tggtgctatt ggaggaggtc
    31741 ttttatttgg cgaaacagga gctatagctg gaagtatatt aggaaagaaa aaaataaaaa
    31801 cttatgtaaa tagtttaaag ataaaaatta caattaataa tataaagaat tctacaaagt
    31861 atatatattt aattaattct aaaatttcta caaattcaag tttatataaa gaatcttata
    31921 attatgccca agagatttta tcaactttgt ctataataac aagttctaag agtatagaag
    31981 ataaaaaaga gtctatatct agttctacag ctgatgaaat attaaaatat aagaatttgt
    32041 taaatatgga agctattaca caagaagaat ttgatgctaa gaaaaaagaa ttgctaaatt
    32101 tataatatat aagcacttac ttaaaaataa gtaagtgctt atattatgtt aaaaaagaaa
    32161 ggaggttaaa aatggcaact atacaaacat ctatccgaat tttcgacgga atgacacctg
    32221 cttttagaca catgactaat gctatgaata ttgtattaag ttcattcgag caattacaaa
    32281 gaacatctag caatgctata gatgctaata gtattagaac agctagagaa gaactagcac
    32341 gtgcagaagc tgggtttgat agattagaac aacaaataag agaagctgat gaacaacaaa
    32401 aaagatttaa tgatgatgtt agaaaaggag caagtagtac agataagtta gtagaaaatg
    32461 ctaaaagatt agtagcaact tatattggat tgagaagtgt tggtggtttg attaatttaa
    32521 gtgaccaaat gacgagtact aatgcgagac tagctatgat aaatgacggg caacaatctg
    32581 atggaggact caacaaaatg atatttcaat cagcggaaag agcaagagca tcttatttag
    32641 atactgcaaa aattgtaagc agagtgggca tgaatgcagg caaagcattt agtagtacaa
    32701 aagaaatagt agcattcgcg gaacaactta acaagaaatt tgtaattgca ggagcaacaa
    32761 cagaagaaac taattcagta cttttacaac ttacacaagg tttaggaagt ggcgtgttaa
    32821 gaggcgagga actaaatgct gtgtttgagt cagcacctaa tattattcag tctattgccg
    32881 attatctcga ggtcgatata gggaagataa gaggtatggc aagtgaagga atgttaacag
    32941 cagatattgt aaaaaactcc ttactttcag cggcggagca gactaatgca gagttcgaga
    33001 agatgcctta tacatttgga cagatttgga catcaataaa aaataatgca gtcatgattt
    33061 ttggtgttat acagaaaaaa atagaacaat ctatgtctag ccgaggtttc cgaaccttta
    33121 tagataattt tatcagcgga ttatatgttt taggtaatgt attttttaac atttttaatg
    33181 gaattataag tatattaggg agcccagctt ttcaaagttt ttcaaacaca atgattgttg
    33241 ggataagttt aatttcacaa gcactaggct ggatagtaac acaagcatta aaccttgcta
    33301 atatatttgc acaaaattgg agtataatag gtccaattat agcaggggtt gtagggattt
    33361 taggtactta tttgattgca ttaggaagta tatggctttg ggaaacttta tgcaaaatat
    33421 caaaaaatgc cttagcaatt gcttctgcaa tacatgcttt agcaatacat gcagaattaa
    33481 cagaaactca aatggctaca gttgctcaat ggggtttaaa tgcagctatt ttggcgtgtc
    33541 cgattttttg gatagttgca ggaataatag ctttagtagc agtcttattt gtaggagtag
    33601 caatattcaa tcattttgca gggactagtg tatctgctat aggtatagtt gcaggtgcaa
    33661 tatcagttgc agctgctttt gtaggaaatt tatttattgc aacagggaac ttaattatag
    33721 atattgtagc tttaatttat aatagtttag caaactttgc agagttcttt gctaactttt
    33781 tagacgaccc gatcggctct gtcataaggg cggtatctag aatggctgat gcggtattag
    33841 gtatattaag aagcatagca tctgcaatag ataccatatt tggctcaaat ctagcaaatg
    33901 cagtgagtgg ttggcaagat aaattacaag ggtggactga caaagtagca ggagaagcta
    33961 agataaaagt tgaactaatg gaccctaata aactgcattt tgacagattt aattgtggaa
    34021 aagcttggga tgcaggatat aaatggggag ataaattaga aactaatata aaagataaat
    34081 tcgatattag caaaatagca gaaaaggcaa aaaaagattt aggtctagat gacctttggg
    34141 atgacaaata tggattagga gatggatttg gttcagcagg tcttaattca cctttaaatg
    34201 acgcagcaaa aggagcaaaa gatacagcag gaaatacagc taaaatggct aaaacaatgg
    34261 ataaaagtca agaagactta aaatatctta gagatattgc agaacaggaa acaataaacc
    34321 gatttacagg ggtaaacata aaaattgata tgaacaatac aaataacata agtaaagata
    34381 cagatgttga tggaatagtt aatgtcttaa ctgaaaaact gaatgatgct atgattgtat
    34441 cagcagaagg aatagtttag aaaggaggga tataaatggc ttatgacttt tatttagatg
    34501 gagtacaatt accaatacct ccgccaaagt tagagattaa agttacaaat aaaaacaaga
    34561 cagttgattt gataaatact ggagaagtaa acatactaaa aaaagaagga ttatctgaaa
    34621 taagttttga agcagaattt acacataata agttgccatt ctatcgtgga gcttttaggg
    34681 atgttcaatt ctttttaagt aaactggaac tattaaaaac tgattgtaag ccatttcaat
    34741 ttattgtatc gagggaatta ggtaataaag tactatttaa cactaatata aaagtatctc
    34801 ttgaagagta tgctatttca gaagatgcag aaaatggctc agatacaaaa gttgcaataa
    34861 agttaaagca atatagagat tactcaacta aaaagttagt tcttgcccct cctaaaaatg
    34921 agactggtag acctaatgta aagatagagc caaaacgagt tgattcagtc aatgccacaa
    34981 acactaaaac atatacagta aaagcagggg atagcctttg gtcaatttgt cagaaacaac
    35041 ttggtaatgg ttcattatat aagaaagtat acgaattaaa taaatctatg atggataagg
    35101 caaataaggg gaagaattta agtaaataca ccatttataa agggcaggtg ttaaaacttg
    35161 gttgatgaat tagtgttagc aaatgataga gatgtaagat tagtaatagc tcattgggaa
    35221 gatttctacg aacctgctgt cattgatggt atcacatggg agatagaaag aagaggaaca
    35281 ccttctaagt tagaatttac aatagttatg gatgatatat tagagttttg cgaaggaaat
    35341 tctgtaaggc tgtattataa aggaataggt atcttctatg gatatatatt tcagaagaaa
    35401 agagataaag aaaatcacat aaaaattgtt gcttacgacc agttgagata ttttaagaat
    35461 aaagatactt atgtatataa taatcaaact gcatctgaac ttgtaaagat gttagctaaa
    35521 gattttaatt taaaatacaa tgtcatagaa gatactaagt ataaaatatc taggatagaa
    35581 gaaaataaaa cactctttga catgatacta acagcactag atgatactct aagagagaaa
    35641 aaagaaatgt atgttttata tgatgatttt ggaagaataa cattaaagaa tgttgcttct
    35701 atgaaacttg atacggttat gaacaatgat gtaattgagg actttgacta caattcttct
    35761 attgatagtg atacttacac aaagattaaa cttgtgagag ataatgaaga aacagggaaa
    35821 agagatgtat atattgcaca agactctaca catatgagga gttggggaat acttcaaatg
    35881 tttgatacag tagataaaaa catgagtgaa gcagaaataa aacaaaagtg tgatatactt
    35941 ctaaaactat ataataagaa aactaagtca ttaagtttaa aaaatgtact tggagatatt
    36001 agagtaagag caggttgttt agtacctgtt tttttaaatc taggagatat taaacttcaa
    36061 aattatatgt tagttgagaa agtaaaacat acatttgaaa ataattcaca tttcatggat
    36121 ttgactcttg ttgatggaga tgaatttgct tcttattctt caagctcata ttcaagtgga
    36181 aatactaaca ataaggatga aaagaaaaat ggtcctgcac aaagtactac gaaaaaaaat
    36241 acaggtaaaa aagttcctgc tatatttact gcatattatc cagggaacaa tgcaatggaa
    36301 ggtggaaaaa cagattgcaa tggaaagcca cttgatgtaa aatcaagaac tgttgctggt
    36361 ccaatgaatc gagaaggagt taagaaaact tggtatactg atgattttct aaagaaacat
    36421 ccagtttttg aatatggaga taaagtaaaa attatacttc ctggtactgc ctatgacaac
    36481 aaagtatata cagttaaaga taatggagga agaatatatg ttgaaacaaa cggaacatat
    36541 catatagata tactattagc taatgctagt gaatgtaaaa aatttggtag aaagaatggc
    36601 tatataatta taggtggaga tgaagaacaa acatatcaag ttgaaggtaa taaccaaagt
    36661 agtacaaata ataactctaa agaagataaa ttaattagta tagcaaaaag taaactgggt
    36721 tgtaattatg tgtatggagc agaaggtcct aataattttg attgcagtgg gtttactcaa
    36781 tggtgttata aacaaatagg tataaaaatt cctcgtactg cttctgcaca aagtaaagca
    36841 ggaaaagcag tagatttaaa agatagaagc aagtggaaag caggagactt attatgtaga
    36901 attggtggag gaagtagtaa tcatgttgta atgtacattg gaaacaatca aataattcat
    36961 tcaccacaaa caggagatgt ggtaaaaata gagtctgtta attcttatag aaaaggaaaa
    37021 gcatacacac atgtgagaag atttatataa gtgaggtggc aatatgagcc aagatttatt
    37081 acagataata aaaaaagctg caatggatgc agtagaaaca agcaacccaa tgagggttgt
    37141 atttggaaca atagaaagta ttagtcctct aagagttaag atagaacaaa aactatctat
    37201 tggtgaaatt tttctaatac aaacagatac atttaaaaga tatacagata aaaaaatagg
    37261 agataaatta gtcttaattc gtatgcaagg aggacaacaa tacttgattt tagataggat
    37321 gtgatgaagt gttaccaagc gataatttag attatgacat tgaagatgta tcaataatta
    37381 attttgatgt aaggcaagaa ccaagtaaga cctttaaatt aaatatagaa aaatctaaga
    37441 tagatggtat ttgtgatgat gttgaagcat taaaacaaac catcttttta attttaaaca
    37501 cagagagata ccaacatcta atatatagtt ggaattatgg agtcgagttg aacgacctta
    37561 ttggagagcc tatatccttt gtaatccccg aacttgaaag acgaatcaaa gaagcactaa
    37621 ttcaagatga tagggttgaa aatgtagata attttgagtt tcaaaatgta aagggtaaag
    37681 tacattgtaa gttcacagtt tacagtaaat atggagatat aaaagcagag aaggtggtga
    37741 gtgtataatt gtttgagtta atgacatttg aaaatataat taaaagaatg ttagatagtg
    37801 taccagatac ttttgataaa agggaaggtt ctataatata caatgctctt gctcctgttg
    37861 cagtggagct tacagaaaca tacattgcaa tggatgaatt actagaccaa acattcgtag
    37921 atactgctag ttattactat ttagagaaga gatgtaaaga gagaggtatt acacctttac
    37981 ctgcaaccaa tacaattgca aaaggagttt ttaatataga tattcctatt gattcaagat
    38041 tcaacttagg agagtacaat tatgtggcaa ttgagagaat atctgaaaaa acatataaaa
    38101 tgaaatgtga aactgctgga cctatatttg agttagggca attaatacct atcgaatata
    38161 tagatggtct tgaaactgct gagttaacag aaatactaat aaatggagaa gatgaagaat
    38221 cagaggacag tctaaggcaa cgatactatg atagtttaaa ctcacaaagt ttcggtggta
    38281 atatacagaa ttataaggat gaagttaata aattgcctga cgttggggga gttaaagtat
    38341 atccagtttg gaacggtgga ggtactgtta agttagtaat aattaattct aacttcaaag
    38401 ttccatctag tgatttagtt aatttagttc aagaagaaat tgatcctcta caaaaccaag
    38461 gagaaggtct tggattagca ccaattggac accgagtcac agttgaagga gttacaagta
    38521 caactataaa tatatcagca gagataacat acaaaaatgg ctacacttgg gagaatataa
    38581 aatcaattgc agaagaagca atagacgact atttaaatga acttaacatg agttgggaag
    38641 atgaagaaaa cttaatagtc cgtatatctc agatagaaac tcgtttgtta agtatagatg
    38701 gagtgttaga tattacaaac acaatgataa atgaggttaa atctaatcta acaataaata
    38761 gtaacagtat agtagtgaga tgtgaggtag ttggataaag agattaatct aataaattac
    38821 ttaccacaaa ttctgcaaga taaagaagaa tatataaaag tatttaatgt agaaaataaa
    38881 gaaataaaaa cactacatga aaaattaaat gacctatcaa atgaccagtt tttagaggat
    38941 ttaactccaa gtggcataaa aagatgggaa aagataatgt ctataactcc taaaagtaat
    39001 gagagtttag aagatagaag gtttaggatt tttagtaaat atataagtaa attaccttac
    39061 tcagagagat ttttaaggaa ctggctagat aatgtagttg gagaaggcaa ttatgaatta
    39121 actattaata atgctactta taacatacac cttgagagtg atgctagaaa tcaagattgg
    39181 tttgaggagg ttcattcttt tgtaagtagt attaaacctt gcaacatgac tttagattac
    39241 actagagtgc ttgtaagtaa agacaattat atgaattttg gtataacaac cctaataggt
    39301 caagaaataa ctatataccc ttggagtcca ccagatatag aaacttatgg agaaattgat
    39361 gtattaactg gcaatggagt tggataccaa gagataacaa tattttagga ggtgatatat
    39421 tggctataga taaaagttat tacactataa ttacagatgt aggaaaagca aagatagcaa
    39481 atgcaagtat cacaggtaat aaagtgggat ttgtaaaaat tcaacttggt gatggaggag
    39541 ggagtgaata tactccaact gagagtcaga cagctctcaa aaatgtggta tgggaaggca
    39601 atatcggaaa tacaaccaca gatgaaactg caccaaattg tataatatta gagagtttaa
    39661 taccatcaag tgtaggcggg tttatgataa gagaaatagg atatttagat gatgaaaata
    39721 atttaattgc catttctaaa tacaaagagt gttataaacc ttctatagaa caaggtgcag
    39781 tggtagacat gaaggttaaa actgtgctta ttgtatctaa tgtaaataat atagaactta
    39841 aaattgaccc aacaataatc tttgcaacac tcaaagatat acaggactta gaaactaaaa
    39901 taggtactgt taatactaaa attgatacaa ctaaaacaga gttaaacagc aaagtaggag
    39961 acacaacact acttactaca acagataaaa caaatatagt taatgcatta aatgaggtaa
    40021 aaaaaactag tgtagatagt atagaaacaa cagcagagaa aacaagttat aataatgcaa
    40081 caagtaaact taatgctaca aatgttcaag gggcgattga tgaaatagta gcagaggtaa
    40141 gaggtaatag aagtagtatt atatctagta taaatgataa tttgatacca atgtagaaag
    40201 gatgtgatta aatgcctcca gcggagacgt tcatatgtaa tagaattgtt aaaaaaagaa
    40261 gaggttatta ttcagaacga gatgtttttc tttcaccttg tccttatgtt tatggagaag
    40321 gcggtatgta tgagtcaact tactatggtg agtttgattt aagtaattct aaatatataa
    40381 cagttcctac aagtaccaaa tatgaaaaaa ctgcaactcg tgtttatttt atatctggtg
    40441 gaaatatgat aactagtagt agtggaatga aacaagctat tactcttgaa cttataccag
    40501 accctaatat tattataaat gatgatttag gtgttattag tgattcttgt aatataaatt
    40561 ataggattcc agacagtaat acaagtgtaa aatttgatgt aactgaaaaa ttaaatggtg
    40621 ttgtaatatc taaaaaaaac tatgctcttg atggcaatta tactttaaat cttacagatg
    40681 aacatttatc aactttaagt tttaactcta caaataatat aactatagaa cttagtactt
    40741 atcaaggggg taagttttta gaaaaaacgg ttacatttac aaaaggtaat actaaaccaa
    40801 aattaaatat aacttcttat aattcaacta ctgcaatctt tacagcaata gacatagata
    40861 ataacttgtc taaaatagag tggttcattg atgatgtatt aaaagaaaca ataacaacag
    40921 atttgtattt agagaaaaca atcaactatg aacttacaga caatgcaata cacacattaa
    40981 aaatagttgc tacagatgca gaaaatgcaa ctgttgagaa ggttttaagt ataagcaaag
    41041 agataatgcc acttcaagaa gatgctagtt taagtgatat atcaactaag ttagcagaga
    41101 ttggagaaga gtttagaaat ggtaaaacaa gtattataaa tactttagca ttaaagaata
    41161 tagaagcaag tttgaataac acacttgttg agttatcaga gaaaataaaa acaagttttg
    41221 atagttcaga cgctagtgtg caggatttgc aaaatagaat aacagaattg aataatcagc
    41281 ttagtcaaag aaaaaaatgg gctacaggta gatatacatt tacagattta gatatttcaa
    41341 attttacctt aaactctgaa tcaattgttc agacgaaatc aattataaca gatttaagtt
    41401 tcacaccaag tataataata attgattcta tacaaatgaa aagtggaact gatagagttt
    41461 attttcgatc tataacaaat ttagatatta ctattggtgc aaaatataca aattcatccc
    41521 ttccagtagg aggaagtgga tatatttata tacaaaaacc tacacctagt aataattttt
    41581 tattaatttt aaccagatta gatggtcagg gaagagaaat aagctttagt ccaattgttg
    41641 gggagacttt gacatggtat gcatttgaat aataaggagg taataaaatg aatagaggaa
    41701 atagaataat ttataatcaa gacggaaaga tattactcca aacaggagaa gcaacaggag
    41761 atatattaga acacgacaca ataacagaat taaatttcat agatttagaa tttggaagta
    41821 tagactatag taaacagtat atagagtcta taaatccagt tacaaaagaa ccagttttaa
    41881 aaaatataga gattgttttg actaatgaac aaaagagatt agaagtatta gaaaaagagc
    41941 taagcatgtt aaaagaagaa aataaaaata gagatagtga gatagtaaac actgcttttg
    42001 aaatagaaaa tataaaatta aataataaca tataggagga attaacatgt ataatttatt
    42061 aaaacttatg atagaacaaa agaactatag tactaaagag gatttgcaac acaaaatgga
    42121 tgtattctat gcagtaaata ggattacaga agaacaatat ttagaactaa caagtttatt
    42181 aaagaaagaa gaaataccag cagaaccaac agtttaagag aggttctttt tttatttcta
    42241 aaaatatata aattcattta cattttgcct acacttcatc tacactttgt agacgtttat
    42301 agttttaaaa ttaggtacaa taaaactata aattaagaaa gaggtggatt aaatggcaac
    42361 agtttatgaa tttaattata caggaagtga acaacgtgct acattaaaac ccggcaagta
    42421 taaattagaa tgttgggggg cttgtggtgg tagatataaa gctgatgatt ttactacttg
    42481 tgcaaaaggt ggttatgcaa aaggtgaaat tatattaaaa gagaaaacta attttcgtat
    42541 ttgtgttggt cagtcaggtt atgaaaaagt tctatctact tcttctttta ctagaactgg
    42601 ttataatggc ggtggtgctg gtggatattc taattttgct ccttacaaat ggtcatttga
    42661 tggtggtggt tcaactgata taagactttc tagtggtaat tcttcttggg atgatttaga
    42721 aggtttgctt tcacgtatac ttatagcagg tggtggcggt ggtgctagtg aatcttctgt
    42781 tagtaaactt ggtcatggtg gtggcttaaa tggtgataac ggaattggtt atcaaggtaa
    42841 tttcgcaggt ggtggcacac aatatcaagg tggtattaat aatagttcag attttgatgg
    42901 tttatttggt aaaggtggta gttataataa acatataggt ggtggtggtg gatggtttgg
    42961 tggtgcaggt tgtgttacaa ctcaaagtgc tgctggtggt ggaagtggtt acgcactaac
    43021 taaagatagt tacaaaccac aaggatatat tccatcatca gaatattggc tagaaaatgt
    43081 agttatgacc gctggaggta atactactaa agcggatggt tatgctaaaa taacattact
    43141 acaagcatta ccatttttga atatatcctc atataattcc acacaggcaa tatttaaggc
    43201 tgaccacaca gaccctacac tattaactaa gatagaatat tttatagatg atacattaaa
    43261 agaaactata acaacagact taacaacaga gaaaacaatt aactatacat tagaagataa
    43321 tgctctacac acacttaaaa tagttgttac agacaatgcc aatgctacag cagaaaaagt
    43381 tgtaagtata agcaaaaata taatgccatt agaggatggt gctagtttac aggatatagc
    43441 aactaaagtt agcgaaatga aagatggctt aaaaagcgga aaaagaagta ttgtaaatgt
    43501 attaacatta aaggatatag atgcaagttt aaacaataca tttgttgaat tatctgaaaa
    43561 ggtaaaagat tattttgata acgcagatta ttttttccaa gaggctaaca aaggaaaaga
    43621 attaattgca actaccattg gttcacctgc aacaaaagaa aatacttatc aagaattatc
    43681 aagttatata actgaattta aaaatactat aggtacactg actactcaac ttgaaagtgc
    43741 taacaataaa ctgacagacc cttatattac aaaagtgaat aacagaggtg ttccaagtac
    43801 ttttacagtt gattttcgca tgtattatcc atcatcatca tttactcgta gaagtacttg
    43861 tatatatgtt gatgctactt ttattcacta tactacacca ggagatatga gtacaggtgt
    43921 tcataaaagt gttaaatatg ttaaaactga tactaaagat ttgaatggtt atcgaatatt
    43981 ttatgatggt gatggaactc tcagcttaac ttatgaaaat aattctgtga gggttgttat
    44041 acaaggagga tatacaagtt cacctggaag aaaaaaatct gatgtttatt cttataatgt
    44101 tcgtatggtg aatacttatt gggattaata aagatattaa aaaagtatag gtgtttgtat
    44161 gccaatagaa ttaaataaaa agagcttaga taattctaag ttcttttttt atgtaaaaat
    44221 taggaggtag tatggaagaa atcagcataa atctattatg tgcagtagca ggggttataa
    44281 tatcctactt agcatttaga aatagctcaa acagaaagat acaagatgaa acagaaacaa
    44341 ctacaaaatt agaacaacaa acaacttttc tgtgtgagaa tgtaagagat ataaagcatg
    44401 atgtagcgaa gtttaatact agtttcttag atattagtga acgagttgca aaagtagaag
    44461 caagtacaaa acaagcacat cttagaattg atgaaattat aaatagaatt ggaggaaaat
    44521 aaaagatgga taatttaata agttttatac cagagcagtt gctaatttta gtagctgctc
    44581 tctctattat aggcaaaggt tgtaaaaaat ataagcaatt agataataaa tacattccaa
    44641 ttatattact tgtgttggga attggatttt caatatggat gctaggattt aacccttcat
    44701 caattttaca aggtatttta tgttggggag ttgcaatagg agcaaatcaa gtttataaac
    44761 agttgaagga ggaaaataaa taatgaaagt agtagtagta ccaggacata cactaacagg
    44821 aaaaggaaca ggggcagttg gttatataga tgaaggaaaa gaaaacagaa ttttaactga
    44881 tttaattgta aaatggttga aacaaggtgg agctattgtg tatactggaa aagtagataa
    44941 atctaataac tatttagcag aacaatgtga aattgccaat aggcaaaatg tagacttagc
    45001 agtacaaatc catttcaacg caaataaaac aactctaaat cctatgggta cagagacaat
    45061 atacaaaact aacaatggta aggtatatgc tgaaagagtt aacaaaaaac tatcaacaat
    45121 atttaaaaat agaggtgcaa aatcggacgc aagaggtctt tactggctta gtcatacaaa
    45181 agcaccagca atattaatag aagtgtgctt cgtagatagt aaagcagata cagactatta
    45241 tatcagacat aaagacatag ttgctaaatt aatagcagaa ggtattttaa ataagacaat
    45301 agataataaa gagaatggtg aggggaaaat catgtacaaa catacaatcg tttatgatgg
    45361 agaagttgac aaaatccctg caactgtagt tggttggggt tataatgatg ggaaaatact
    45421 gatatgtgat ataaaagatt acgtaccagg tcagacgcaa aatctttatg ttgtaggcgg
    45481 tggagcatgt gagaagatag gttctattac taaagagcat tacacaatga taaaaggtaa
    45541 tgatagattt gatacacttt ataaggcatt agattttatt gatagataga ttgaggattg
    45601 aataaaatcc atttacactt ttcttacatt tcatttacac ttttaataca aatatactat
    45661 aaaaatacga tataattaag acagttaaat ttataattaa tattagtaag aatctttaaa
    45721 ataaatatag caacaggtat atcataacaa ataaatgacg agaattagag atagttaatc
    45781 atgaggataa ctgtctcttt tttattgtcg aataaatcag agagtgaagg gattgaatag
    45841 aatgagaaaa cttgataata ttaagaaggg cactataatt ttagacataa ttcctaatag
    45901 tgcaaatggg agagatgcaa tgaaaattga aaaaattgaa attaaaggta ttggtggaat
    45961 aaaagaatta agtctgaggt ttaataaagg acttaatata atatgtggtg caaatggtat
    46021 aggaaagaca actattttag aagttatttc acatcttttt tctatacaat cttctgattt
    46081 aaagaaaaat gcaaaatttg atttaggaga agcaatagcg gattattcgt ttgaagatat
    46141 agaagctgta cataaaagta cttatcaaat caatgatttc aatgcttctg tgaaagaact
    46201 tatttttgat aatcgagata aaaaaaaata tagtaaattt gtgttgtttt ttaagacaca
    46261 tagagaatta aattattcaa aattggatgg aataactgga gatgaaacat atagtgatga
    46321 tagtacatct tatatggctg atttaggtat agattcaagt aaaataaaaa attggtttat
    46381 taatagactt cttttttcta agcaagaagg ttctcttaca aaagaacaat tatgtaattt
    46441 agacttagca atagaaatga ttagtatttt agataagaat gtatcatatt ctagaattgt
    46501 atcagattct ttagatttaa tgataaatac tcctcaagga gaaatttata tagaatattt
    46561 atcatcagga tataaatcat gtttttatat attattaggt ataattaagg aattagaata
    46621 tagatttact aaaccttata ttgaagttaa ggattttgat ggtgtcatac taattgatga
    46681 gttagactta catctacatc cagaatggca agttaaaatt gtaaatgcat taaaagtatt
    46741 gcttccaaag gcacaaatta tagcaacaac acatagccct aatatgatac aaacactatc
    46801 accagatgaa attataccat taaccatgga tgaaaatggt aatgtgcgta aaaaagactt
    46861 agagttaggt gaatatggat tgcaaggatg gactattgaa gaaatattga cagatgtcat
    46921 gggaatgaaa acaacttctt ctgagttata tttagataca atgaaaaaat ttgatagagc
    46981 tatggataaa gagaatgtag atgaaatcaa gaaatactat gatatcttga tgaaaatgtt
    47041 acatccaaat agtacactta gaacaatact gaaaatacaa gtggcaggaa taatagattg
    47101 ataaaaataa atagaacaag aaagcctatt gaattaacag aagaagttca gaaacaatta
    47161 acagaagaat ttaaaaataa taaagaaaaa agcgtgtgga ggaaaaagta tattacaaat
    47221 gctctacttg agatgtctca tggaaaatgt tgttattgtg aaatgaaatt gatagaagag
    47281 ggcaaatcac tgaatgtaga gcattttcac cacaaagaca aatatcctga tgaagtagtg
    47341 aaatgggaaa atttattgcc atcttgtggg agatgcaata gtgaaaaggg tactcatgac
    47401 actaaattag aacctataat aaatcctgca ataaataacc caaaagatta tttatattta
    47461 tataattata gatataaaag taaaaataga aataaacttg gtagtgatac aattgatata
    47521 ttatatttga atgatacaga caaattaatt aaaccaagag taaatatttg catggcttta
    47581 ttcaaaaggt tagagtctat aaaaacattt gttgaagatt atcaaaatgg tacaggaaca
    47641 catacaagaa gaaaaaataa aattataaac gaagtaaaag gtgtattaag atgcgctcaa
    47701 ccaactgaag aatatagtgc tattgtggca aatgttatag taaatgatga tgattataaa
    47761 tacataaaag aaataatgtc agaatttaac ttgtgggata atgaactaga agaattagaa
    47821 agtaaagcaa aagaaataat gttagattca gaatgaaata catattaaaa taactagata
    47881 caattaaatt tgaaagtata aaaaaagaag gtgataaaaa tttctttatc atcttctttt
    47941 tttatacttt ctttctttaa gttttttctt tttcatatct tctaatacat ctaatgcagt
    48001 agtataaaat tgtatttcat ctctgtaagg cacagctata tcaagagatg tattaatagc
    48061 tataaatatg agcactatta tagatgagga ttgcagaatt aaatttatat aattatcttt
    48121 aatatttaat gaacttaaaa ataaattagc aagtaatgaa aaaacaacag ccataaggga
    48181 gaaaaacaat gcattaccag gattattatt tttctctaac tgacctttaa ttctagtttg
    48241 ctcaatttct atctttctaa ttgtattaat attcttaatt tcattgtaat gattataaat
    48301 ttttgtgtaa tattcttgat aatttattac actttcttta tttcccttaa agtattttat
    48361 atccttcttg atttgttttt tcttttttat atttttaaac caatatttca aactcataac
    48421 ccctccataa atccatataa tatactttaa tttataacac acttaataat atatatcagc
    48481 aatttgtcga acgattttaa ataaaaatag agccaggata caagagaaaa atttacagag
    48541 atttattctg atgatgtgtg gtcaacaatg gataaggcta gataatttgt aaagacaaaa
    48601 ttataagttt tatatttata aagataaagc tttgatatat ttcatccata attttcaaag
    48661 acttatataa aaaggagtgt tttatttttc taaacactcc tttttatata agtctttcaa
    48721 taatcaattt ttaaaattaa taatattgct attatttctt tttcaagttt atagtttctt
    48781 catttttatt ccaagatata attactttat aattagattc cctatttgaa taaagacctg
    48841 gtctttgtct ttttattatt ttttcttgac ctgattctaa aactgcatct tttaaaatta
    48901 taattgcatc ttcaccatct ataaactcat atgaataatt aaatttttgc ttaactacat
    48961 ctttactgtc tttatatttt atttctaatt ctgattgttc ttcagtagta acaaatttag
    49021 ctgtccaatg ttctgattct ccaataaaga tatttttatc attttttata ttaagtacaa
    49081 ctatcgcagc gattactaaa actataaaaa tacctagaaa aagttttttt ctcatactga
    49141 gccctccttt gaataattat atgtattata acatgaaaac agttatccaa catcattatt
    49201 atgaattttc tgaatttttt gatatttata ttctatcatg atagaatatg tggtataatt
    49261 aataaaaaag gagggattag tatgaattct aggtttaaaa aatctgcaac aagtatttta
    49321 tctttacttg caatgacaat actattacca gcaagtgtaa atgctagtga agtagaaaat
    49381 tcatctatga aagtaacata ttcagagaaa caagtaacag atatgaacaa gttatatgat
    49441 atggctaaaa atggaatcac tgatgttaaa agtcccaacg aaaaaggtat aatcaaaaat
    49501 gaaagaacag gagaatctat gtatgttgat gctatatcaa caacacaatt attagaagtc
    49561 aaacaatcta aagatttaac atctaagata tattctaaaa cagttttttt agatactgat
    49621 acaataaagc cccttagaaa agcaagtata tcagaagacg gatgggataa aactggtggt
    49681 gtaaaagcat ctggtacagt tacatataca atatcaaaag gagatggagc attccaatat
    49741 atttctgtca gtaaagtttc tggaaactgg tctattgcag atggtcagat tcgtgtaaca
    49801 aatagaaagg tgtacataaa ccaaactgga tttgtttcta atggattagg aattaagcaa
    49861 caacaaagga aagacccaac atcaaattct ttttcatata atgcaccttc tagttggaaa
    49921 ccagtgggta gagattctct tcaatataat tgtgggtttg aatcaattgc aacattagta
    49981 agaggaggaa gctcttggtc attcaatttc atcgttcaac cttaaaacat tttaagtgaa
    50041 ttttattaaa attaaagata tattgacacc ataaagatat aataaaaaat aaaattatag
    50101 taaaaattgt aataataaag cgaacgaaaa ttatcatact aaaacataag acatgttata
    50161 attgtattat atatacatta acatacacca aagcactctt ataaaaagag tgcttttagt
    50221 gtatattttt ttactttttt gaatgatttt tgtcgtatga atatattctg aataaggctt
    50281 tatgataaaa taaatttaaa ggagtctttt tatattaaaa attaatattt taaatattta
    50341 gaatttataa aactttaggg agataaaact gatgttgtta ggagatgaaa ttaagttgaa
    50401 tttagagcca agtgatttaa taaattctat accatacttt aaatctgaat attcagattt
    50461 atgtggttta gacgcagatt ttataaagga acagttattg aaagctgaaa ataatgatag
    50521 aatagtgagt atggctcgag gagaaggaag agcatttgtt gtggaaccaa ttattaagcg
    50581 taataattat tgcaaattta gaaataattc atatgatatt tttaattcaa ttccatctta
    50641 tgaatatgca ataaaaataa tagaacgcta taaaaaccat attttcaatc caactgaata
    50701 tcgcaacagt gtaatgagaa gagtaggaac atatgaaaat gatattttca attggggata
    50761 caaatcaatg cactcttata tggaatcatt acctttaaga gaatttttag agccaaataa
    50821 agttgataga acattattat ttgagtttct tgattttatg agagaacttt tttcatatca
    50881 gttaagaacg tatccaggac gttttgataa ttccaaaact ttagatgcca tagagaccat
    50941 aaaaaatgct atcataaaaa cagaatcaca tgatgatttg cttataatta aagaatggtt
    51001 atgtttttta ttgcatactg gaaatcatag agtatttaaa aatataactc catggatttc
    51061 attgtctaca ggaagcaaaa gatataatat tgtacataaa tttgcttctc gcaaaccatt
    51121 tacttcttat agaacaatat ctgagaaaca tacttataac gaaaaagaaa agtttatatt
    51181 tattgattat tgggagtatg tagataagga aaatttaact tttaggaata caaattacat
    51241 tgtcaataaa cttaaaagta tgggagtaaa ttggtatgaa aatagaaata atgaagttat
    51301 ggtaaaatat gcattatttc ctcaacaaat tatagggtat tattattttg agaatagaaa
    51361 attacaatat tactttatta atcctcatta tttgaaagca tggcaaaaaa ataaagattt
    51421 taaaatagga gatgaactgt acataaatca agaacaagta gtgtttcctt ttaataatcc
    51481 gtatagagta atatacggaa aaaaatatgg acatatttca gtagttgata agagataaat
    51541 atttttaggc tctaattata aataaaacaa tattatttat ctttttttaa aattaa
//
LOCUS       DAENCV010000012        50511 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAENCV010000012 DAENCV010000000
VERSION     DAENCV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.012"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            2..75
                     /locus_tag="KQD82_000646"
     tRNA            2..75
                     /locus_tag="KQD82_000646"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Met,seq:cat)
     gene            90..166
                     /locus_tag="KQD82_000647"
     tRNA            90..166
                     /locus_tag="KQD82_000647"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Ile,seq:cat)
     gene            184..260
                     /locus_tag="KQD82_000648"
     tRNA            184..260
                     /locus_tag="KQD82_000648"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:His,seq:gtg)
     gene            269..344
                     /locus_tag="KQD82_000649"
     tRNA            269..344
                     /locus_tag="KQD82_000649"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Lys,seq:ttt)
     gene            352..425
                     /locus_tag="KQD82_000650"
     tRNA            352..425
                     /locus_tag="KQD82_000650"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Cys,seq:gca)
     gene            433..509
                     /locus_tag="KQD82_000651"
     tRNA            433..509
                     /locus_tag="KQD82_000651"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:467..469,aa:Arg,seq:acg)
     gene            522..597
                     /locus_tag="KQD82_000652"
     tRNA            522..597
                     /locus_tag="KQD82_000652"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Val,seq:tac)
     gene            673..1086
                     /locus_tag="KQD82_000653"
     CDS             673..1086
                     /locus_tag="KQD82_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379143.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1395..4826
                     /locus_tag="KQD82_000654"
     CDS             1395..4826
                     /locus_tag="KQD82_000654"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG4379144.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLEDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5068..6996
                     /locus_tag="KQD82_000655"
     CDS             5068..6996
                     /locus_tag="KQD82_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG4379145.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGALSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7449..7913
                     /locus_tag="KQD82_000656"
     CDS             7449..7913
                     /locus_tag="KQD82_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG4379146.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            7920..8414
                     /locus_tag="KQD82_000657"
     CDS             7920..8414
                     /locus_tag="KQD82_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG4379147.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8430..9455
                     /locus_tag="KQD82_000658"
     CDS             8430..9455
                     /locus_tag="KQD82_000658"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG4379148.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9442..11889
                     /locus_tag="KQD82_000659"
     CDS             9442..11889
                     /locus_tag="KQD82_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG4379149.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12069..13442
                     /gene="radA"
                     /locus_tag="KQD82_000660"
     CDS             12069..13442
                     /gene="radA"
                     /locus_tag="KQD82_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG4379150.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13445..14515
                     /gene="disA"
                     /locus_tag="KQD82_000661"
     CDS             13445..14515
                     /gene="disA"
                     /locus_tag="KQD82_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG4379151.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14604..15698
                     /locus_tag="KQD82_000662"
     CDS             14604..15698
                     /locus_tag="KQD82_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG4379152.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            15827..18004
                     /locus_tag="KQD82_000663"
     CDS             15827..18004
                     /locus_tag="KQD82_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379153.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(18190..19035)
                     /locus_tag="KQD82_000664"
     CDS             complement(18190..19035)
                     /locus_tag="KQD82_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379154.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            19252..20418
                     /locus_tag="KQD82_000665"
     CDS             19252..20418
                     /locus_tag="KQD82_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG4379155.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20440..22086
                     /locus_tag="KQD82_000666"
     CDS             20440..22086
                     /locus_tag="KQD82_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG4379156.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            22183..23556
                     /locus_tag="KQD82_000667"
     CDS             22183..23556
                     /locus_tag="KQD82_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4379157.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LDELNTK"
     gene            24001..24954
                     /locus_tag="KQD82_000668"
     CDS             24001..24954
                     /locus_tag="KQD82_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG4379158.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            24992..25954
                     /locus_tag="KQD82_000669"
     CDS             24992..25954
                     /locus_tag="KQD82_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG4379159.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            25979..26965
                     /locus_tag="KQD82_000670"
     CDS             25979..26965
                     /locus_tag="KQD82_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG4379160.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            26978..28024
                     /locus_tag="KQD82_000671"
     CDS             26978..28024
                     /locus_tag="KQD82_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG4379161.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            28191..29921
                     /gene="lpdA"
                     /locus_tag="KQD82_000672"
     CDS             28191..29921
                     /gene="lpdA"
                     /locus_tag="KQD82_000672"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG4379162.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEKLVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            30339..33287
                     /locus_tag="KQD82_000673"
     CDS             30339..33287
                     /locus_tag="KQD82_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4379163.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            33265..33729
                     /locus_tag="KQD82_000674"
     CDS             33265..33729
                     /locus_tag="KQD82_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4379164.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            33842..34144
                     /locus_tag="KQD82_000675"
     CDS             33842..34144
                     /locus_tag="KQD82_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4379165.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            34206..35570
                     /locus_tag="KQD82_000676"
     CDS             34206..35570
                     /locus_tag="KQD82_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG4379166.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            35605..36078
                     /locus_tag="KQD82_000677"
     CDS             35605..36078
                     /locus_tag="KQD82_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379167.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            36250..36900
                     /locus_tag="KQD82_000678"
     CDS             36250..36900
                     /locus_tag="KQD82_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG4379168.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            37074..38006
                     /locus_tag="KQD82_000679"
     CDS             37074..38006
                     /locus_tag="KQD82_000679"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG4379169.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            38280..38993
                     /gene="ispD"
                     /locus_tag="KQD82_000680"
     CDS             38280..38993
                     /gene="ispD"
                     /locus_tag="KQD82_000680"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG4379170.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            38990..39475
                     /locus_tag="KQD82_000681"
     CDS             38990..39475
                     /locus_tag="KQD82_000681"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG4379171.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    39570..39778
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            39842..41557
                     /locus_tag="KQD82_000682"
     CDS             39842..41557
                     /locus_tag="KQD82_000682"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG4379172.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            41679..43124
                     /locus_tag="KQD82_000683"
     CDS             41679..43124
                     /locus_tag="KQD82_000683"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG4379173.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            43562..45043
                     /locus_tag="KQD82_000684"
     CDS             43562..45043
                     /locus_tag="KQD82_000684"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG4379174.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            45264..45419
                     /locus_tag="KQD82_000685"
     CDS             45264..45419
                     /locus_tag="KQD82_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379175.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            45564..46961
                     /locus_tag="KQD82_000686"
     CDS             45564..46961
                     /locus_tag="KQD82_000686"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG4379176.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            46946..47359
                     /locus_tag="KQD82_000687"
     CDS             46946..47359
                     /locus_tag="KQD82_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG4379177.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            47465..48223
                     /locus_tag="KQD82_000688"
     CDS             47465..48223
                     /locus_tag="KQD82_000688"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG4379178.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            48236..48973
                     /gene="rlmB"
                     /locus_tag="KQD82_000689"
     CDS             48236..48973
                     /gene="rlmB"
                     /locus_tag="KQD82_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG4379179.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            48976..49503
                     /locus_tag="KQD82_000690"
     CDS             48976..49503
                     /locus_tag="KQD82_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG4379180.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            49568..50215
                     /gene="sigH"
                     /locus_tag="KQD82_000691"
     CDS             49568..50215
                     /gene="sigH"
                     /locus_tag="KQD82_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG4379181.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            50289..>50511
                     /gene="tuf"
                     /locus_tag="KQD82_000692"
     CDS             50289..>50511
                     /gene="tuf"
                     /locus_tag="KQD82_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG4379182.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETP"
BASE COUNT        20702 a         5310 c         9503 g        14996 t
ORIGIN      
        1 tggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
       61 ctcctattac cgctactgaa atttaatgtg ggccattagc tcagttggtt agagcgcccg
      121 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacatt aaattaactt
      181 atggtgggta tagctcagtt ggttagagcg ccagattgtg gctctggagg tcgtgagttc
      241 gactctcatt atccacccca acttatatgg tctattagct cagtcggtag agcacctgac
      301 ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaaagg cggcgacata
      361 gccaagtggt aaggcagtgg actgcaactc cttgatcccc agttcgaatc tgggtgtcgc
      421 ctccaataaa atgcgctcat agctcaactg gatagagtgt ctgactacga atcagaaggt
      481 taggggttcg agtccctttg ggcgcaccat atgaatagcg agggattata gctcagctgg
      541 gagagcacct gccttacaag cagggggtca caggttcgag ccctgttaat cccaccagtg
      601 caaacctcta gtaaaactac tagaggtttt atttttgatt gaaaaagttt ttatatttag
      661 gaggtaaaaa atttgtatat agtaatagga aatgaaatag ttgatagtga agaacttaaa
      721 ataattatag ataaaaatag tgaatttaaa gttgaaaaag atttaagtaa aagcacaaaa
      781 agagaagatg taatagcata tcagttaagt atagatttaa attacctaga ttctcttata
      841 aatgagcaat gtaatttagc cagtctatct gatgaagaga aatttgatga atatatgact
      901 ttgtctgatg aacttgcact ggatttagaa gagttaatgc caaaatatac aataataaat
      961 gctagagcat ataaattaga cgaggtatat ggaattgtaa aaataatact agctgtagcg
     1021 tatgcagatt tagggcactt aaaactttct gatgtagtaa agaggctatc tagacaagta
     1081 gattaggata tgaatatgaa ctatattaaa aaaatgtata aaatcttacg taaagatgaa
     1141 aataattatg taaacataga aaaaaacagt ataaaatcaa gtcaattgta gaaattttca
     1201 cataaattca tctgaaattc tataaaaaat gccttaattt taatgaggta aatcgagttt
     1261 acacagtcta aaaattactt atatttataa atttatctta ttaattaagg aactttctta
     1321 aacatcttca aaaataaagt attttagtct ataatcttta atattacaca aacaataaag
     1381 gagaagaata gagtatgctt aaaaagttta ataaaatact agtggctaat agaggcgaaa
     1441 tagctataag aatatttaga gcctgttcag aattggggat aaaaagtgtt ggtatataca
     1501 gtaaggaaga taagtatgga ttgtttagaa caaaagcgga tgaatcatat ttgataggag
     1561 aaggtaaagg gccaatagat gcatatttag atatggatgg aataatagac ctagcaaaga
     1621 gaaaaaaagt tgatgctata catccaggat atggttttct agcggagaat gcagaatttg
     1681 ctcgtaaatg tgaagagaat ggaattactt ttataggacc atcatctaag gtaatgaata
     1741 tgatggggga taagattaac tctaaaaaaa tcgcaaaaga agttaatgtt caaactatac
     1801 caggagtaga aaaagcaata agaagtactg aagaagcaaa ggaagttgca aacaaaatag
     1861 gatatcctgt tatgataaaa gcatctaatg gcggtggtgg tagaggtatg agaattgtac
     1921 atcgcgaaga agacttagag cttgaatatg aaactgcttg tagtgaatca agaaaagctt
     1981 ttggagaaga tattatattt atagaaaaat atatagctga tccaaaacat atagaagttc
     2041 aaatattagg tgataattat ggaaatatag ttcacttata tgaaagagat tgttcagttc
     2101 aaagaagaca tcaaaaaata atagaatatg caccagcttt ttcattagaa gataaagtaa
     2161 gaaaagagat atgtgaagat gcagttaagt tatcaaaaca tgtgggttat tctaatgcgg
     2221 gaacattaga atttttagta gatgcaaatg gtgggcatta ttttatagag atgaacacaa
     2281 gagtacaggt tgaacatact gtaacagaga tggttacagg aatagatata gttcaaagtc
     2341 aaatactaat agcccaaggt tacagcttag atagtgaaga aattaatata aaatcacaag
     2401 atgatgtgga aataagaggt tactctatcc aatgtagaat aactacagaa gaccctaaga
     2461 ataaattcat gcctgataca ggtaaaattc aagtttatag aactggttca ggatttggta
     2521 taagactaga tggaggaaat ggatttacag gggcaaacat aagtcctcac tatgatagtt
     2581 tattggttaa gacaatatct tgggatagaa cattccaagg agcaataaat aaaactataa
     2641 gatctataaa agaacttaga gttagaggtg ttaaaactaa tgtaggattc ctagttaatg
     2701 ttttaaataa tcctatattc tcaaatggta aatgtagtac taagtttata gatgaaaatc
     2761 cagatttatt tgaaataaca gaaagtaaag acagaggaac aaaattactt caatttatag
     2821 gagatgtaat tgtaaatgat aatgcttgta aagaaaaacc attatttgat gcattacatg
     2881 acccaagaat ggataaggat ggttcaaaat cggaaggaag taaaatactt ttcgataagt
     2941 taggtaaatc tgcatatata gaaaagataa aaaatgataa gaaattacta ttaacagata
     3001 caacaatgag agatgcacat caatcattat tagcaacaag aataagaaca tatgacttat
     3061 taaaagctgc taagccaaca gaaaaatatc aaaaggattt attctcactt gaaatgtggg
     3121 gaggagctac ttatgatgtt gcatatagat ttttaaaaga atctccttgg cgtagattac
     3181 aaaagctaag agaagaaata ccaagcataa tgttccaaat gttgcttaga gcatcaaatg
     3241 gagttggata taaaaactat ccagataatg taatagaaga atttacaaaa gaatctgcta
     3301 gacaaggtat agatgtattt agaatatttg attcattaaa ctgggttgaa aatatgaaac
     3361 catctattaa tacagcttta gaaactggaa aaattgttga agcaactatg tgttatacag
     3421 gtgatatact agataagact aaaactaaat acaatctaga atattatatt aaaatggcac
     3481 aagaattaga aagcttggga gcagacataa tagcaataaa agatatgtca ggtcttctta
     3541 aaccatattc agcttacaca ctagttaaag agttaaagaa aaatgtaaaa gcaccaatac
     3601 atttacacac tcatgataca agtggaaatg gtgtagcaac ttgtttaatg gcgtcagaag
     3661 ctggagtaga cataattgat gctgcattag aatctatggc aggacttaca agtcagccat
     3721 cacttaatgc tatagtagag gcacttaaga atacagagag agatacagga atagatttat
     3781 ttggatatga tgagttagga aaatattata aggatttaag aaaagtttat aataaatttg
     3841 aaagtgattt aactaattct tgtgcagaaa tatataattt tgaaatacca ggaggccaat
     3901 atacaaactt aaaacctcaa gctgatagtt taggattagt taatagattt gatgaagtta
     3961 aagaaaaata taaagaagct aatgaagttg taggagatat aataaaagta actccatctt
     4021 caaaagttgt aggagactta gctatcttta tgactaaaaa taaattagat aaagacaata
     4081 taatagaaga aggtaaaaat ttatcattcc cagattctgt tgtggattac tgtaaaggta
     4141 tgattggtca accagaaggt ggaataccaa aagatttaca agaggtagtt ctaaaaggtg
     4201 aagaagctat aacagtaaga ccaggttcat tattaccagc agaagatttt gatgaaatag
     4261 ctaaatatct aaatgaaaag tatgatataa atgctaatat aagaaatgtt ataagttatg
     4321 ctttatatcc aaaagtatat gaagattata tcaaacatct acaacattat aatgatatat
     4381 ctaaacttga aagtgatgta ttcttctatg gattaaataa aaatgaagaa tgtgaagtag
     4441 aaatagaaga aggtaaggta ttaacaataa gattagttga aattggtgaa gttaaggaaa
     4501 atggatttag aactatagga tttgagttaa atggaatggt tagagaagta gaaataaaag
     4561 ataagaattt ctctggaaaa attaataatg ttgaaaaagc tgatatgaat gaccctcttc
     4621 aaattggagc aagcatacca ggaaaagtta taaaaataat ggttaaagaa gaagatgaag
     4681 taaaagcaaa tcaaccatta atcgttatag aggctatgaa gatggaaact ataatagttg
     4741 ctaagactga tggagtaatt aagtcaatca aagtaaaaga agatgatatg gttgaagata
     4801 aacaattatt aatgataatg aagtaattta attaaaataa attaaaaagc actctgaaat
     4861 gtttaataaa aaatgtttta gagtgttttt atttatttta attaaatatt tgttaaaaaa
     4921 aagaggacaa ttacctccag atgtagaaat aaagctagta aggaaaatgt tatctaaaaa
     4981 ttctaaatat agtaaatatt ttccataata tttcttagga aataacagca aagataaaat
     5041 acataaattt aaaaggtggg tgcttatatg aaggtttatg atagtaaaat gttgagaaat
     5101 gtagcggtat taggacatag cggatgtgga aaaacaaatc taatcgaaac tatagcttac
     5161 acagcaaaca ctaataagat acctaaatta acagacaagg taaatatgac ttatagcatg
     5221 ggattaatac ctatagagta taatgattat aagtttaact tattggatac cccaggatat
     5281 tttgacttta gcggagacgt tgtgtcatct cttagagcaa gtgatgcagc aataatagta
     5341 atagatgcaa cagcgcctat tcaagttggt acagagaaat cattagaact aactgagagt
     5401 attcctaaga taatgtttat aaataaaata gataatgaaa aggcaagata caaagatgca
     5461 atagcaatgt taagagaaaa atacaataat aagatagttc ctatgataag tcctatatac
     5521 aaagataaaa actttgtaaa gttacataat gtttttgaaa acatagatga tttagaaggt
     5581 gaatttaagg aacaagctat gagtgttaaa gaagcattaa tggaacttat agcagaaact
     5641 gatgaccaaa tattagataa atattttaat ggagaagaac taactacaga agagatacaa
     5701 aaaggtatta ttataggtat acaaagaggt gatattatac ctgtaatatg tggttcaact
     5761 ataaataata taggcacaaa ggaaatatta gatacaatat caagttattt agaacctatc
     5821 tttacagagg aaagcaaacc atttagggga ttagtattta aaactatggt tgacccattt
     5881 gttggaaaga tgtcatatat aaaaattact gagggtgcac tttctaagga taaagatgtt
     5941 tttaatataa ataaaaatgt aaaagaaaaa atagctaata tatatacatt gagaaatagt
     6001 gaattagtag aaatagaaaa agctaaagct ggtgatatag tagttataac taaagttaat
     6061 tctctaaaaa caggagacac tatatctgca gataaagatg cagaagcttt agaaaagata
     6121 gattttccta aaccacaaat atattatgca gtaactccta agaataaagg tgatgaagaa
     6181 aaagtagcat ctgtattaaa taaactagta gaagaggacc ctacattaca ttggtataga
     6241 aatacagaga caaaacaagc acttttagga ggtcaaggag aacttcatat aaaaacaatt
     6301 aaaaataaaa tgaaagataa gtttggtgtt gatgttgaat tgaatgacct taaagttcct
     6361 tatagagaaa ctataaaagg aacagctgat gtacaaggaa aacataaaaa gcaatcaggt
     6421 ggtcatggtc aatatggaga cgtaaaaata agatttgaaa gatgtgaatc tgattttgaa
     6481 tttacagaag aaatatttgg tggttctgta ccaaaacaat atataccagc agttgaaaaa
     6541 ggtttaaaag actctatgca aaaaggtata ttagcaggat atcctgttac aaacataaaa
     6601 gctacgcttt atgatggttc ataccatgat gtagactctt ctgaaatggc ctttaagatg
     6661 gcagcaagtg cagcctttaa aaaaggtatg gaagaagcac acccaatact actagaacct
     6721 ataatgaaac ttaagataac tgttccagaa gaatatatgg gagatgtcat gggtgatata
     6781 aataagagaa gaggaaaaat atttggaatg gaaccagatg acaaagggaa acaaataata
     6841 tttgcagaag ctccacaagc agagacattt aaatatgcaa tagatttaag agcaatgaca
     6901 caaggtagag gatactttga aatggaatta gagagatatg gagaggttcc atcacagttt
     6961 gcagaaaaaa taattgggtt agctactgca aagtaatgaa caaaataaat agaaaaataa
     7021 ttaatatata ttattaaaaa tatttttagg gttttaacta ggtataggtt tatgttaggt
     7081 aaacttatac ctagttttta tacatttttg atgaataaaa aaatatataa tactttctta
     7141 aatattttaa taaaaagaaa gtattttttt attattattt ttacaaatgt ttagtaataa
     7201 agatgagaag gtatatttaa aatataaagt atttaaagtt ctgaaatcta atcatgaagc
     7261 aaaaaattag catttggtta tcaataaata taataaataa aaactcataa ttaatgaaag
     7321 aataaattca tatctaaatt tctataaaaa atttaaattt atacaattta gggctttaaa
     7381 aatttaaata ataggtgtaa aataatagtc aaagaaagtc aaaaataaaa aggtgtgtgg
     7441 tgatattaat ggcaactatg acagatataa ttgaaaaatt tattaaagat ttgatggaag
     7501 aagataatag catacaaatt caaagaaatg aactagcaaa cttgtttagt tgtgcaccat
     7561 cacaaataaa ttatgtctta acaacaagat ttacaataga tagaggatat tacattgaaa
     7621 gtaaaaaagg tggcggagga tatgttcaaa tagaaaaaat acgaaaaagt aaagatgggc
     7681 atattagaga actactaaat gaaaaaatag gaagtcagat atcctataaa aaagcaaaag
     7741 aattattaga aggtttaaaa gagtcggatt tgattaacga aagagaatta aaattaatat
     7801 tatatgcaat agatgataaa tcattgtgta tgccaatata tgaattaaaa gagaaggtaa
     7861 gatctaatat tttaaagaat ataataatag gattatttag tatagaggag tgataattta
     7921 tgctatgtca aaaatgcaat aagaataaag catctgtata ttataataag atagtcaatg
     7981 gagaaaaaac agagatgtat ttatgtagtg agtgtgcaaa agagaataca gaaatgaatt
     8041 ttaacttaga tatgcctttt tcaatgatgg atatattttc taatttaggt tttcaaccta
     8101 aaaaagaact tgaggaaaag ctagtttgtc ctaaatgcaa tacaacatat agtgagttta
     8161 agaataatgg gagatttgga tgtagtgaat gttataatgc atttagtagt caagtaaatc
     8221 caatgcttca aaatatacat gggcatatag aacatacagg aaaagcacct aaaaaatctt
     8281 tttataagat aagtgtagaa aatgagatta aagaactaaa agaagattta gatagagcta
     8341 taaaaaatga agagtatgaa ttagcagctc aatttagaga taaaattaaa tatttaaaag
     8401 gttctattga ctaagattgg agtgatatta tgaaagagaa catagtcatg aaatcaagag
     8461 ttaggctagc tagaaattta aataactatc catttccaaa taaattagat aaagaatgtg
     8521 ccatggaaat aatagaaaaa gtaaaaaatg cgtttattaa ttctaactta gaacaaaaag
     8581 aagaatttga tttctataaa attgaagatt tagaccaatc taaaaaaatg cttatggttg
     8641 aagaacatat aataagccca gatttagcag aaaatgataa aagtgcagtt atagtaaaaa
     8701 aagataaaac tataagcata atgataaatg aagaagacca tataaggata caaactatat
     8761 gtgatgattt aaacttagaa tatgcatata gtgtagcaaa tgaaatagat gatttgttag
     8821 aatcaagttt agaatatgcc tttaacacaa agctaggtta tctaacatct tgtccaacta
     8881 atacaggaac aggaatgaga gcttctgtaa tgatgcattt accagcatta agtcaactag
     8941 gctatatgga tgaactttat aaaatttcat cacaaatagg tatagcaatt agagggatat
     9001 atggagaacg aacagaagca ttgggaaata tatatcaaat atctaatcaa cttacacttg
     9061 gaagaacaga gtctaatatt atagaaaatg taagtggact tacaaaagat gcaatttcaa
     9121 aagaaataaa agctagagaa atattgcaaa aaaaattagg tataaaacta gaagataaaa
     9181 tatttagatc gatagggaca cttgaaaatt caagagtaat gagtagcgca gaggcaatga
     9241 gccatctatc aaatataaaa atgggaatag aaatgaacta tatagataaa ctagatttaa
     9301 gggctataga acaacttatg ataggtatac aaccagcgca tcagtcaata atgtataaga
     9361 gtgatgatgt tgagaataga gatataaata gagcaacata tattagagaa actttggaaa
     9421 aattaagagg agggatgaat tgtgaacttc aatagattta cacaaagagc taaaaaggca
     9481 attgatttag cgtttgaatc tgctaaaagt ctaggacaca atattgtcgg aagtgaacat
     9541 atacttttag gtcttttaag agaagaagaa ggtatagctg ctaaagttct aagtaaagtt
     9601 ggatttacag aagcttattt agaaggcaaa atagttgata tggaaggtaa gggagaagaa
     9661 atctcagaag atatagtatt aagtccaagg agtaaacaaa tacttgaatt atcaggaatg
     9721 tttgcaaata aattaaaaac aaattatatt gggactgagc atatattatt agcaattatt
     9781 caagaaggtg aaggaatagc taataagatt ttaaattatg ctggagtaaa tgataggact
     9841 ttagcccaat taacaattga tatgatgggt attagtgata aaaatcaata caaagcagaa
     9901 aatagttaca cgggcaacca gaatcaagca gaatctaaag tacttgataa gtatggaaga
     9961 aatcttacac tatatgcaaa acagaataaa atagaccctg taataggtag agaaaaagaa
    10021 atacaaagag taatacaaat attaagtaga agaactaaaa acaatccagt gttgatagga
    10081 gacccaggtg tagggaaaac agccatagct gaaggtctgg ctacaaatat agccttagga
    10141 aatgttccag aaacactaaa aaacaaaact ttatactctt tagagatggg ttcattattg
    10201 gctggagcta agtatagagg agaatttgaa gaaagaatta aagaagttgt agatgaagtt
    10261 gttaaaaatg gaaacataat cttatttata gatgagatgc acacaataat aggggctggg
    10321 tctactggag aaggctctat agatgcttca aatatattaa agccagcctt agcaagagga
    10381 gaaatacaag ttataggtgc aactacaatt gatgaatata gaaagcatgt tgaaaaagat
    10441 tctgctctag aaagaagatt ccaaccagtt atggtagatg aaccaactaa agaggactca
    10501 ataaaaatat tagaaggatt aagagataaa tatgaagctc atcataaagt taaaataact
    10561 gatgatgcta taaaggcagc tgtagaatta tcaactagat atatatcaga cagatatctg
    10621 ccagataaag ctatagactt gattgatgaa gctgcatcaa aagtaagatt aaaagaaaat
    10681 acacctcctt cagaaataaa aaaattagaa ctagaaatag aaaatataga taaagaaaaa
    10741 gaagaagcag taagatgtca agactttgag aaagcagcca aaataagaga tgaacaaggt
    10801 ctacttaaaa aacaattaga agatgttaga gaaagatgga acaagtcatc taaacattca
    10861 gatttagttg atggagaagt tattgctgaa gtagtgggct tatggacagg aataccagtt
    10921 aataaaattc ttgaagaaga ggctgataga cttttgagac ttgaggaaat actgcacaat
    10981 agagttatag gtcaagaaca agcagtaaaa tctatttcaa aggcaatcag aaggtcaaga
    11041 gcaggtctta aagaccctaa tagaccaatt ggttcatttt tatttttagg tccaacagga
    11101 gtaggtaaaa cagaattatc taaggcatta gcagaagtgc aatttggaga tgaaaatcaa
    11161 ataattagaa tagatatgtc tgaatatatg gaaaaacatg ctgtgtcaag aatgataggt
    11221 tcacctccag gttatgtagg tcatgatgaa ggtggtcaat taactgaaaa agtaagaaga
    11281 aatccatatt cagttatctt atttgatgag atagaaaaag ctcatccaga tgtatttaat
    11341 attctgttac aaattctaga tgatggtaga ctaactgatt caaaaggaag aactgtagat
    11401 tttaagaata ctattgtaat aatgacatca aatgttggag catctacaat tggtagacaa
    11461 aaaactttag gatttagtat agctaaagga gacgaagaag aaaaatctca atatgaaaaa
    11521 atgaaagaaa atataatggg tgaattaaaa caaagattca gaccagagtt tttaaacaga
    11581 atagatgata taattgtttt ccactcacta aatgaaaatc atatatcaaa aatagtactt
    11641 ttaatggcag cgaaactaca agagagatta aaagaaatgg atataaaatt agaaatgagt
    11701 gatgaggctg ttaaattaat ttctaaatct ggatttgact tagaatatgg agcaagacct
    11761 cttaaaagag ctttacaaaa agagttggaa gatgagctat ctgaagcaat cttaaaaggt
    11821 aatgtgaaaa agggaagtaa tgtagtagca aaagtcaaag atgaaaaaat agtttttgaa
    11881 actaaataat ttaatagagt tgcattttaa aatattgcaa atataagttt tataatctaa
    11941 acaaaatttt aagggcaatg aatctataat aattcattgt ccttaattat tttatttaat
    12001 ctttacatat tgtctttcag atatgctaaa attatagtat agaataaaaa attaaggatg
    12061 ataaaagtat ggcaaaaata aaaacaaaat atgtatgtca atcttgcgga tatgaaacag
    12121 ctaaatggct tggtaaatgt ccagaatgta caaaatggaa tacatttgta gaagaaatag
    12181 accaaaaaag caccaaaaaa gaggtcttta taatagataa atcttcatct aaaccagtca
    12241 gtataaactc aatagagagt aaagaagaag agcggtttac aacagatata aatgaattag
    12301 atagagtgct tggaggaggt atagttaaag gctctttagt gcttgttggt ggtgaccctg
    12361 gtataggtaa atcaacactt ttaatccaag tatctagtaa tgttgccaat ttaggaaaaa
    12421 ctgttttata tataactgga gaagaatcag aatctcaaat aaaaatgaga gctaaaagat
    12481 taggtattaa ctcagaaaat ttatatatat ttgctgaaaa taatttaagt ataattgaat
    12541 cttatttaga aagtgtaaat ccagaattaa taattctaga ctcaattcaa actgtcttca
    12601 gtccagaaat atcctcggca ccaggaactg tcagccaaat taaagaaggt acttcaaaat
    12661 tcatgaaaat ttctaaaaaa atgggaattt caacgttcat agttgggcat gttacaaaag
    12721 aaggttcact agcaggacca aaattattag agcatatggt tgatactgtt ctatattttg
    12781 aaggagaaag atataatact tatagattag taagagctgt taaaaatcga tttggttcaa
    12841 ctaatgaact tggagtgttt gaaatgaggg atttaggatt agtagaactt gataatccat
    12901 ctaaaatttt aatctctgaa aaaccaaaag atgtagcagg ttcagtaata atatctactg
    12961 tagaaggtac gagaccaatg ttacttgaat tgcaagcatt agtttctcct acaagttttg
    13021 gtataccaaa aagaacatct acaggagtcg attacaatag agtaggtatg ttattagcag
    13081 tattagaaaa acgtgttggt ttgcaaatac aaaatcaaga tgtctatata aacatagttg
    13141 gaggaataaa gataaatgaa ccctcaattg atttagggat agccatttct gtagcatcta
    13201 gttttagaaa tataccgata gatgaagata tagctgttac aggagaagtg ggacttacag
    13261 gagaagttag agcagtaagc tttatagaaa agagaatagc tgagtgtaaa aaacttggat
    13321 ttaaaaagat agtagttcct agaagtaatt atgatgttgt aaaagagacc aaaggcatag
    13381 aaatatggcc agtagataac ttaagacaag ctataaatat agtacttggg aggaatcaat
    13441 aaatatggag aattttctag ataataaaaa tatgctatat gcattaaaaa tgatatctcc
    13501 tggaactcca cttagattag gtctaaacaa tgtactaaga gctaagactg gtggattaat
    13561 tgtaattgca acaaacgaag atgtaatgaa aatagtagat ggaggatttg ctataaatgc
    13621 agaatattca ccatcatatc tatatgaatt agctaaaatg gatggagcta tagttttaag
    13681 tggtgatgta aagaaaatat tatttgctaa tgcacaactt atacctgact attttataga
    13741 aacatcagag acaggaacaa gacatagaac agcagaaaga gtagcaaaac aaactggtgc
    13801 tatagtcata ggaatttcac aaagaagaaa tgttataaca gtttatagag gaaatgagaa
    13861 gtatgtagtc gaagatatat ctaagatatt tactaaggca aatcaggcta tacaaactct
    13921 ggaaaaatat aagacagtat tggaccaagc tgtaacaaat ttaaatgcct tagagtttaa
    13981 tgatttggta actatttatg atgttgcatt agtcatgcaa aagatggaaa tggtaatgag
    14041 agttacaagt ataattgaaa aatatgtgat agaattgggt gatgaaggaa ctttagtaag
    14101 tatgcaatta gaagaattaa tgggtacaac cagaatagac cagaaattaa tattcaaaga
    14161 ttataataaa gaaaacacag aaataaaaga acttatgaaa aaggtcaaaa atttaaattc
    14221 agaagaacta atagaattgg tcaatatggc aaaactatta gggtatagtg gtttttcaga
    14281 aagtatggat atgcctataa aaacaagagg ttataggatt cttagcaaaa tacatagact
    14341 accaacagca ataatagaaa acttagtaaa ttattttgaa aactttcaac aaattttaga
    14401 tgcatctatt gaagaattag atgaggttga aggaataggt gaaataagag caacatatat
    14461 aaaaaatgga ctcataaaaa tgaaacaatt agtcttatta gatagacaca tatgatgaat
    14521 tttaaatgaa ttttactact tttttcttga atataatata cactttggtc tataatagat
    14581 aagtataact agggggtgaa gcattgataa ggaaagtaac aagaatattg tttattttac
    14641 ttggatttac aataggtata acaacctatt taacattaat gaaagatttt gaaatattaa
    14701 catttggaaa agaaacatat ggttatatcg cagctgtagt agcaggtata ataatagcca
    14761 ttttaggata tcttatagaa ccatgggttg taaataaagt aaaagaaatt gctaaaatag
    14821 tggataaaga attgtcaaaa tatcctcaga ctgatatatt gttagggtct atgggtctta
    14881 tagtaggatt tgttattgcg tatttattaa gtggattagt aaatagaata ccaatagtag
    14941 gtggaatact atccttacta ttatatttat tcctaggata tcttggaatg aaagttgcgc
    15001 ttaaaagtaa aaatgattta tttaatattg gaaaactagg aagattagca aatcctatta
    15061 aggataaaga taaggataaa gaaaataaaa aagaagtaaa agccattcct ccaaaagtat
    15121 tggatacaag tgtaataatt gatggaagaa tagctgatat ttgtaagact gggtttatag
    15181 aaggaaaact tattattcct gcttttgttt tggaggaact aagacatatt gcagattctt
    15241 cagatgattt aaaaagagta agaggtagaa gaggtctgga tatattaaat ataattcaga
    15301 aagaattaaa tatagaagtt gaaataagtg agagagactt tgatgatata gcagaagttg
    15361 atagtaaatt attaaagctt gctcaggttt taaatggaaa agttgttaca aatgattata
    15421 acttaaataa agttgctcaa ttccaaggtg ttgaagtatt aaatataaat gagttagcta
    15481 atgctataaa accagttgct ataccaggtg aagatatggt tgttcaagtg gttaaagaag
    15541 gaaaagaggc tcaacaaggt gttgcatatc tagatgatgg aactatgata gttgttgatg
    15601 gaggaagaaa atatatgaat gagactataa aagtactcgt tacttctgtt cttcaaactc
    15661 ctgcaggtag aatgatattt gcaaaaccta aaaattaata tgttcaaaga aacctataat
    15721 ttgggctgtt tattttaaat agatggtcca aatttattta tttataacga taatgtttaa
    15781 tgtattctta aagatgggga tttaaatcag aaagcaggga gaatttatga gaaaaaagat
    15841 tatatctttc ttagcaacat ttatcataat attaacatca gcaagtcagt atagttttgc
    15901 agatgatata tcaacacgtg gaaaagtaat atttatagat atgaatagaa ctagtatgag
    15961 taatatgtta agaataaaat cattaagaga agaactagac aatagagggt atattggctt
    16021 gatgaatata agaggcgata aaggaagtga tgatagaagg tcatatgcta gtatgggtgc
    16081 tggtggtaga gctaatgtgg ctaatgaaga agatattaat tttgaaagct cgagtaagga
    16141 tagaaatata gtttttgaat cagctacagg aaaaagtgca aaaggtataa ataatttaac
    16201 tataaataag tctataaatg aaaatttaaa ctttggagag tatggttctg tattagggtc
    16261 attaggacaa agtttatctg ataatggatt aaaagcatct gtattgggta attcagatat
    16321 aattgaaaat ggtcaactta taaaaaatag aaacttatgt ctaactgcaa tggatgaata
    16381 tggaagaata cctaatggaa atgtagatac tataaataaa aaagacttat ctatgcctta
    16441 tggaatatca actgattatg ataaattgat tgtagagact aaagaagctt acaaaaataa
    16501 tgatgtgatt tttgtggagc ttggagacac atatagatta gatttatata aaccaaattt
    16561 aaatgaaaaa acatatgaga gtatgaaaga taacatagaa aaaaatatag atatatattt
    16621 aaaaaatata ttttctatgg ttgaagaaaa tgacactgtc tacatagcaa gtgcatttcc
    16681 aagtgattta gattataaaa ataaaagaag actttcgcca ataataaaat taaatggaga
    16741 agggaaaggt atattatcat cttcaactac tagaagagaa ggtattgtaa caaatttaga
    16801 tgttggggtt gaaatattag acaactttaa tataaaaaat caaaatatgg ttggaagaaa
    16861 atatgaactt attaacagag atgataataa agaatttttg atggatgaat atcaaaagat
    16921 agtatctatt tcaagcataa ggtctacaat actgaatggt tttgtaagta tagttttctt
    16981 gtcatggata gttgctatga ttgcaatttt atttagaaag catatatcaa aaaattataa
    17041 agaaacaaca ttttttatat taaaggaact tttaaaaata ggaatagtaa tgccactgtc
    17101 atttatgata acaccaataa tgaatttcaa aacaccatta gcaatatctt taggaataat
    17161 tataataaca cttactatat atcttataag taaagtttta ataaagaacg atttaaaaaa
    17221 catgctgttt tttactggat taaccatagt aattatggtt attgatgctg ggtttggctc
    17281 atatttaatg aaaagcaatg ttatgagtta tgattgtata attggagcta gatattatgg
    17341 agtaggaaat gagtatcaag gtgtggcaat cggtagtgca atatttacat ttgcaatttt
    17401 acttacatat aaaaatatac caaagtggtc tataatagtt ttttctttgg ttatcttaat
    17461 tacaagtgca tctccaatta tgggagcaaa tgttggaagt gctatttctg aatgtgttgc
    17521 atttttacta tttatactat tgatatataa tgtaaagata gatttcaaaa aaatcgtatt
    17581 attaggtata gcagtagtat ttgtattagg agtttttgta gcaatagata tgattttggg
    17641 aagtaattca catttaggta tgtttgtaaa agaaatttac tttaatggac caggagaaat
    17701 aatacaaaca tttagtagaa agatagagat gaatttaaag ctagctcaaa ccagtgcatg
    17761 ggtaaatata ctgttaactg gaataggtgt aattttagtt cttatgatta atcagattag
    17821 atactttaag caactgatgg atgagtatcc aattgtattt aaaggattta tagcatcaat
    17881 agcaggatgt ttagtaactc ttttagtgaa tgactctggg attgtgtcat cagctacagc
    17941 ctttatttat gtaattgtac ctatgataac attaagtata aatcttacag cattaaaaga
    18001 ataagtactg tatgaataat atgtaaatat taatacatta atttaacttc tttatttaca
    18061 aaataaagaa gaaagtttat aaaaaataaa ttataataaa aaagagttta tctcaaaatt
    18121 gattattcta atcaaatttt aagataaact ttttttatta taaagataaa taacatataa
    18181 cctcatattt taggtagatt tttttcttat atgtttagga gtgcatttaa atttttcctt
    18241 aaaaattctg taaaataatt ttgtattagt aaatccatga gcttctaata tactgcataa
    18301 ggtatcattt gtgttaatta aatctttata aatatgagaa atacgcacat catttaaata
    18361 ctctaagtaa gtttgtccca tatactgttt aaattttcta caaaaatatt ctggttgtaa
    18421 gtgtgctacc ttagcgattt tgttaataga aataggcatt ttgtaattaa tctttgtata
    18481 ttctaaaact ggttcaaatt ttgacaaatc aagtactgtt tttttcatat ttttattacc
    18541 tacagatatt ttaaagtttg tatataattc aaataaaagt tcaaataata agcttgtaaa
    18601 gagcagatta gctgcaggag gagaactaag tttaatttct ctcatgtttt ctaatataag
    18661 tttaacttta cttgaatttt ttttatagtt attgtcattg gcattaacct taaagtcaaa
    18721 atagcaattt ttaaaatcag ggatatattt gtttagaaaa gacaatggta cttgcaataa
    18781 tatagaggta ttttgataaa tacatctagt tgagtgaata attccagaat taatgaacat
    18841 acactcatta gcttttaaat tataagtgta accctccatg ttaatttgta aatcaccaga
    18901 agttatataa attatttcta atgagttatg ccaatgattt gctacataac taccactatc
    18961 atcaaatagt ttgaaattaa catctaaatt atcaataggt tcaattattt catgtgaaaa
    19021 attatttctg ttcataataa tatcctcctt ataattttaa attataaata gtaaatttat
    19081 aatgttcaat attaaaaaga taatatagac taataaatta gataataaac atacttaaga
    19141 aaacggataa aatcaaatat aataactgta tattaaaata acttaaaaaa attattttat
    19201 tataagtgca aaatatacct atttaaataa gataagaggg gaggatttta tgtgtatata
    19261 aaaggtgtaa atttaggagg ttggttagtt ttagaaaaat ggatgacctc aagtttattt
    19321 gaaggcacag aagctgaaga tgaatactat ttaccaagac agctttcaag agaggcatat
    19381 gaatctagaa ttaagactca tcgtagtgaa tacatcacag agagagattt tgcaacgatt
    19441 aaatctatgg gatttaattc agttcgtata ccagtaccat actttatttt tggagattgt
    19501 gaaccattca taggatgtgt taaagaactt gataaagcat ttgcttgggc agataagtat
    19561 gggcttagta tattgataga cttacataca gttccaggaa gccaaaatgg atttgataat
    19621 ggaggaatat caggtatatg tagctggtca caaaatccag aatatgtagc gtttacatta
    19681 aatgtattag aaagacttgc aaaacgttat gggatgaggc atgaattgta tggaatccaa
    19741 attttaaatg agcctataac agaaagaatg tggaacataa tgaatgttcc aaatcggttt
    19801 aaggctgtag ataaggagat ggcaaggggg agtaaaccta attcattaga gtttttacgt
    19861 gatttttata taaaagcata taaggtaatg cgtccttata tgagagagga aaacgttata
    19921 gtatttcatg atgcatttga attaaaagca tggaaagact ttatgagaga ggaagaattt
    19981 aaaaatgttg ttctagatac acaccaatat ttgatgttag ctgaagcaga tggatgtgaa
    20041 caaagtattg atagctatct caaatatatt agagaaaatt atgctaaaga tattttacaa
    20101 atgcaaaaat attttcctgt tatatgtggg gagtggagct tatttaattc atatgcttgt
    20161 ggtatagata caaatggagg tcaatcacct ctaaatggga ttgaatcaaa tatagataaa
    20221 ttatcaaaag acgataaaag agaattatat aggaagatag caaaagcaca gcttgatgca
    20281 tggagaaatg gaagtggtca ctactattgg aattataagt tattactaga tacagttaat
    20341 gaagaaggtt ggataggatg ggatagttgg gacctaggca aatgtgtagc tcaagaatgg
    20401 tatcctatag aatattaaac taaaattgga ggaaaagata tgattaaaaa ccctatactt
    20461 ccaggtttta atccagatcc ttgtatttgt agaaagggag atgattatta tcttgtagtt
    20521 tcatcttttg aatggtttcc aggtatacct gtttatcact ctaaagactt aaaaaattgg
    20581 gagttatata ctcatatttt gacagatgaa acaaaaatag atttgaagaa gcttccttca
    20641 tctaaaggaa tttgggcacc ttgtttgact tattgtgaag aagaagattt attttacata
    20701 gtttatggaa ttatgaactc tatgaatgca agatattttg atgttgataa ttatttaatt
    20761 acatcaaaag atataaaggg agagtggagt gaaccagtat atctacactc atcaggtttt
    20821 gatgcgtcta tttttcatga tgatgatggt aaaaagtgga ttgcatcatt agactgggag
    20881 actagagaag gatatgaaaa acctggtgtt atatgtttag tggaatactg tacaaaaaag
    20941 aaagaaatag taggatatcc aaagagaata tggtcagggg gaactgatag aggttgtatt
    21001 gaagcaccac atataacaaa gcgtggagat tactattata taatgtgtgc agaaggtgga
    21061 actggatatg gtcatagtgt aactatggga agggctaaaa atatatgggg accttatgaa
    21121 aaagattcaa tgaatccaat tgtaacttca attccaggcg atttttatga aagacatgac
    21181 cctgaccatc taaagcctaa atactataat ccagaatcaa aacttcaaaa atctggtcat
    21241 ggaagctata tagaaacaac atcaggtgaa gtatatttag ttcatctaac atctagacca
    21301 tttgtaccag agttaaggtg tactttaggt agagaaacag ctattcaaaa aatgaaatgg
    21361 acaaaggata attggcttcg aatggaagat gaaagtaatt tggcaaaaga atacgtatca
    21421 gaaagtaaac tagaagaaca tttagtttca tctataccaa gttttgatga ctttgattca
    21481 aatgaattag gtttacagta ctatgctcct aggatttcac cattatcatt tgcagatgtt
    21541 aagtctagac caggatatgt aaggattaga ggacaagaat caagaacatc attaaataaa
    21601 gtgagcatat tggcaagaaa attaacttca gtatatgcaa gaataaccac taagatggag
    21661 ttttacccag aggtacatca gcatagtgcg ggtttgataa tgtattatga taatatgaat
    21721 tatataaatt taagaaagta ttatagtgaa acattgggtc aaagtgcact atcaataata
    21781 catcttgaaa atggagagaa aacggaattt ttaaatacta ggataccaat aaaagatata
    21841 ccaatatatc ttcgtctgta tgtacaaggt cgtaagtcat attttgaatg gagttatgat
    21901 gaaaaaaact accagagaat aggaaaggta tttgatacaa ctaagttttc agatgagtac
    21961 tgtaaatatg gagaatttac aggtacgttt ataggattaa cttgtgcaga tcgtgtaaag
    22021 cataaacact atgctgattt tgattttttt gaatatatag tagatgaatc aaaagatgtc
    22081 gattaataaa atcattacta aaattttcag agcaaaggta aatcttatgc tctgaaataa
    22141 gaaacattag aaggcaaatc ttacaaaaag gagtatggta ttatgaatac agaagttaat
    22201 gttaaagcaa ctacgattag atatgaaaag atttcatttt ttgaaagaat ttcttatggt
    22261 tgtggggatt tggggtgcaa tatcatatac tcagctatgt cagcattttt gctgttttat
    22321 tatactaatt atgcagatgt tagtgcagca gctgtaggta gtattatgct tgtatctagg
    22381 atactggatg ggtttagtga cttaacaatg ggaattatag tggatagaac taaatccaaa
    22441 tacggtaagg ctaggccttg gattttgaga atggcgattc cttttgctat agcagcagta
    22501 ttattatttt ctgttccatc aaatcttggt atcacttcta aattaattta tatttttatt
    22561 acatataact tagtttctac tgttatttat acagcaatca atgtacccta tgcaactcta
    22621 aattcactta taactcaaaa ccagtatgaa agaggagtat taagtatatt tagaatgata
    22681 ttagccactt gtggtacctt actaataaat ggacttacac tacctctagt tgaatacttt
    22741 ggaaataatt tatcagcatg gactaaaaca ttctttgtat ttggaatagc atctataatg
    22801 gtatttttta taacatttac tggaaccaag gaaagagtaa aagcagttaa gcaagatagg
    22861 aatgaagtaa taccatttaa aataggtatt aaatctttat ttagaaataa gtactggata
    22921 caaataacat tatgtttagt ttgcatattt attgtatttg cgataaatgg tggttcatcc
    22981 gtatattatg caaagtttat tttgggagat gaaaaattat ttggtcctat aaatatggtt
    23041 tcaaatatat cccaaattat agctatgttt atggttgctc cttttataaa aaaatttgga
    23101 aaaagaaatg tattaatagt aggttcaata atattaatat cgtcaaatgt tatgtttata
    23161 atagttggac aaaatcatat aggagtaata tttgcaagtg ctataaaggg aataggaagt
    23221 gctggaatag catcaacaat gtttgctatt gtttcagata ctattgaata tggagaatgg
    23281 aagactggat atagaactga ggggcttata aatagtgcat ctagttttgg gtttaaagtg
    23341 ggaaatggat taggttcagc aatattaggt gcagtattat caattggtgg ttatgtagga
    23401 acatcagcta ctcaaagtga tttagctata gtatcaataa aagtatgttt tatataccta
    23461 cctatattta taacaatatt acaaattatc ataatgtctt tttataagct tgataaagaa
    23521 tatagtacta tacttgatga attaaatact aaataatgaa aaagtttctt ataaagtctt
    23581 aagtttaatt aaggtctatt gtaaatgttc cctataaaaa ctattatata tttatgatta
    23641 ttgaagaatt tttagagaag cgaataataa aaatataaga agactttaaa tgatgaaatg
    23701 gttggttttt tagaaatgat ttaataagtt attgttattt aaagcattat aagtggacaa
    23761 ttaattttta atttatggag atgatattta acaattagaa gtaatatata atatgcacta
    23821 agaagcaatt tttcaactat ttatgaaggt ggtggtacaa gaaaagtatc accttttttt
    23881 tgttatacaa aaaaacagtt gactttttat atgaacccct tctataatgt tactgaaatt
    23941 aagtaaaatg attgaacaaa tgtaaagata attgaaaagc ttgattttgg aggaaagcaa
    24001 atgaaaaaaa ttggagactt aagattaatg atgaagtgtt gttcacttta ttacgaagat
    24061 aatttgaatc aacaggaaat agcaaatcaa ttgggaatat caagaccaac aatatcaaga
    24121 atattaaaag aagcttttga acaaggaata gtaaaaatac agatagttga tgttttgaaa
    24181 aatgattacc aaaaaataga gagaagtttg gagagaaaat ataaattaaa agaagtaata
    24241 gtagtagatg ataaacagga tgcactaaca caaaaacaag aactagcaag agctgtatct
    24301 gaatatttaa ctagagtagt aaaggaaaat gatataatag gtgtttcaat aggaacaaca
    24361 ctaaaagaaa taccaagata tgtggaaaaa agtaactgta aaaatgttac atttatacct
    24421 ctattaggag ggattggaga caatgaaata gatattcatg caaatcaaat agcagtaagt
    24481 ttagcgaggg cttttggagg tgattttaaa ttattacatg ctccagctgt tatgtctgac
    24541 ttgtctacaa aagagaaact ttgcaaagac gaaaaaatta aagaagtact agatttaata
    24601 gataaaacaa ctatagcaat tgtgggtata ggaaatccta tgagtttaaa ctcaacaata
    24661 atggcttctg gatacatgaa tgaagcagat atagaagatt taaaaaaata caactcaata
    24721 ggtgcaattt gtttacaagc ctttgataaa gaaggaaaaa cttctatact tgaatttaat
    24781 caaagagtat taggagtgaa actggaagat ttaaaaaaga taaaaagaac aataggtgtc
    24841 gcttcaggag atgaaaaaat agaggcaata aaggcatcat taaaagcgaa atttataaat
    24901 agtttagcaa ttaatcacag tttagcactt aaattattag aagattgtga ctaatagaaa
    24961 gatagataaa agttaacagg agggaatgta tatgtcaata tcaaaagaaa cattgcttga
    25021 aatgtataag agaatgaatc aggctagaaa atttgaagag aaagttagtt ggttttttgc
    25081 aagaggtatg gttcatggaa caactcactt atctgtaggt caagaagcgt caagtgtggc
    25141 ggctgtaatg gctttagaaa aaggagattt agtatcatta actcatagag gacatagtca
    25201 atttatagga atgggaatag acctaaataa aatgatggct gaacttatgg gtaaagaaac
    25261 tggattttgt aagggaaaag gaggctcaat gcatatagca gatattgagt caggtaactt
    25321 aggagcaaat ggagttgtag gtggaggtct tacaatagct ccaggagctg cacttacaca
    25381 acagtataaa aaaacaggca aaatagtttt atgtagtttt ggtgatggag catcaaatga
    25441 aggtactttt catgaaggaa taaatttatc ttcaatatgg aaattgccaa taatatttta
    25501 ctgtgagaat aatttatacg gtatgtcaac aagcataaaa cgtcatatga atatagaaag
    25561 tattgccact agagcagctt cttatggaat agaaggaata tcaatagatg gatataaccc
    25621 aatagaagta tacgaaacag tacaaaaagc tgctgagaag tgtagaagag gagaaggacc
    25681 tgtattaatt gaaagtagaa cttatagatg gttaggtcat tcaaaatcag atgcaaatgt
    25741 atatagaaca aaagaagaaa tagaatcatg gaaagcaaaa gaccctattg aatttttaaa
    25801 aaattatctt atagaaaata atctatctaa tgaagatgaa ttagataaga ttcaagaatt
    25861 tgcaaagcaa tcaatagaag atgcagtaga gtttgctcaa aatagcccaa atccaaaaat
    25921 agagtcttta ttagaggatg tatatgcaga ctaagaatgg taaaggagga aaataaaaat
    25981 gagtacaagg gaattaacat atgcacaagc aataaaggaa gcaatgtcag aagaaatgag
    26041 aagagatgaa aatgtaatat ttatgggaga agatatagga atatatggag gagcatttgg
    26101 tgtatcagtt ggtatgatag atgaatttgg accagaaaga gtaagggata ctcctatatc
    26161 agaagcagct atagcaggag cggcagcagg tgcagcagca actggtttaa gacctattat
    26221 ggaagtaatg tttatggact ttgtaacaat ttctatggat gcaattgtaa atcaagcggc
    26281 aaaaatgaga tatatgtttg gaggaaaagc acaagttcca atggttgtta gatgtccagg
    26341 aggttctgga actggttcag cagagcaaca ttcacaaagt ttagaagctt ggttttgtca
    26401 tgtaccagga gtaaaagtag ttgcaccttc tacaccagca gatgctaagg gattgttgaa
    26461 agcagctata agagataata atccagtcat atttgttgag aataaattgt tatacagaaa
    26521 aaaaggtttt gtgccagaag atgattatgt aattgaaatt ggaaaggcag atattaaaag
    26581 agagggtact gatgttacag taattactta tggaagaatg ttacaaagtg ttgaagaagc
    26641 agcagagact ctatcaaaag aaaatataaa tgttgaaatt atagatttaa gaactttata
    26701 tccattagat aaagagacta tagtaaagag tgtatgtaaa actgggagag ttttaatttg
    26761 ccatgaagca gctaagactg gtgggctagg tggagaaata tcagccctga taacagaaag
    26821 tgaatcattt gattatttag atgctccagt aaaaagaatt tgtggaaaag atgtgccaat
    26881 accatataat ccagagttag aaaaagcagt tgtcccaaga gttgatgaaa tagaagaagc
    26941 aataaaatct ttaattgtta gataaggagg gatagatatg gtggcaaata aaataaaagc
    27001 cacaccagca gctagaagtc aggcaagaaa agacaatata aaattagata ggctgattgg
    27061 aagtggagaa aatggaagga ttcatttagt agatgtgtta aattatttaa aagataataa
    27121 agcaaatact actcctttag ctagaagaat agcagaagat ttaaatatag atttagaaac
    27181 tattgtaggt actggatata atggaaagat aagaaagtgt gatgtggaaa aattaactgg
    27241 aaaagaaact attgtatcaa ctaatacatc taaatctagt gagaaaaaag agctgaaaat
    27301 tgaaaatgaa aattcaagaa tgtttaacac agtagaagga atatttgaaa agccaaatcc
    27361 aatgagagct actgtagcaa aacgaatgtc agaaagttat tttagtgctc ctgtatttac
    27421 attcaatata gaagtagatg ctacagaatt gaaggtactt agagcaaagt taatcgacac
    27481 agtaaaagaa tcaactggag taaaattaac tatgacagat ttaatagtaa tggcagtatc
    27541 taaaatactt ccaaatcatc aagccttaaa ttcagcatgg acagatgagg gtatatttag
    27601 atataaagat gtaaatatag ctattgcagt tggtttagat gaaggcttat atgttccggt
    27661 agtaaaaaat gcaaacaaaa agtctttaaa agaaatagca aaagaaagca aagaacttgc
    27721 ggaaaaagta aagacaggga aacttatgcc agcagaccaa gaaggtaata catttacaat
    27781 aagtaatgtt ggaatgtatg gtataactac atttacacct ataataaata tgccttcaag
    27841 tgcaatctta ggggtaggtg ctacacaaga taagtttgtt ccagtaaatg gagaggctaa
    27901 aataaaacct ataatgaact tatcattaac atcagaccat agagtaatag atggaacagt
    27961 agctgcaaaa ttcttaaaag atttaaaaga attgttagaa aatcctttat caatgcttgt
    28021 ataggttttg atattttgtt actgatataa atagtttgaa tagattatat tattataatt
    28081 gtttcagtaa atttaaagct tttatatatt aagaattatt tttaacagta actatatgtg
    28141 aatatgattt gcttaattaa aatataagaa aataaatagg aggagagcct atgtcagtag
    28201 aagtaataat gccaaaagca ggagtagcca tggaagaagg tactatagta tcttggctaa
    28261 agcaagaagg cgaagaagta aagattggag aacctatact agaaataaca acagataaag
    28321 ttaatatgga gatagagtca gaaggagaag gtacacttgc tgtaatcata cataaagagg
    28381 aaggtgaagt attaccagta tttacagtaa taggtgtaat agcagaaaaa ggggaaaatc
    28441 aagaggaagt aaaagctaaa tatttatctg gaaatgtttc aaaagaagat acagttaaag
    28501 agaatcaaaa tatagaagtt aaagaagaaa aaataaacaa aaaagagtgt aatcatgatt
    28561 atgatgtagt ggtaatagga ggaggacctg ggggatattt atcagcatta aaagcagctc
    28621 ttttaggtgg aagagttgct ttagttgaag aaaatatact aggaggtact tgcttaaata
    28681 gaggttgtat ccctacaaag acttatataa aaacagcaga aatattagaa gaaattgacc
    28741 aattatcaaa gagaggtgta aaagtaactg tagataaaga acaggacatt aagaaggcca
    28801 taaaatataa aaatagagta gttaaaaaac ttacagctgg tgttggcgga ttattaaaaa
    28861 gtagagatgt agatgtgttt aatcttaaag ctagtgttaa agaagagcat aaagtaattt
    28921 tatcagatgg aaaagtttta gatacagaaa atataataat tgctacaggt tccaaagtaa
    28981 ggatattacc tataaaagga atagaatcaa atttaataat aactagtaca gaagcattag
    29041 atttggaaac agtgccagaa aaattagtaa taattggtgg tggagtgatt ggttgtgaat
    29101 ttgcagagat attcaattca agaggttcta aagtaactat agtagaaatg gaagatagag
    29161 taatcccaag aatggataaa gagttaagcg aatctttaaa atattctctt agtaaaaaag
    29221 gtataaatgt attaactaaa aagaaagttt ctgaatttaa agaagaagga aataacatat
    29281 tagtgtgcat tgaaggtgaa gagccaataa aagcagattt atgtctatat gctataggaa
    29341 gagaagctaa tctttctggc atagaggact tagatattaa aatagataaa ggttcaatag
    29401 ttgtaaatag taaaatggaa acaagtatac caagcatata tgcagtaggt gatgttactg
    29461 gaggtgtaat gttagctcat gctgctttta aaatgggaga ggtagcagct tcaaatgctc
    29521 taggtgtgaa taaagaagtt gacttaggtg cattacctag ttgtgtttat acaattccag
    29581 aagtagcatc agttggtatt acagaagagg atgctagaaa aaaatataat gtaaaagttg
    29641 gtaagtttaa ttttgctgga aatggaagag ctttagcttc tggtcaagaa caaggatatg
    29701 taaaagtagt agcagatgct aaatatggag aaattttagg tatacatatg tttggatgtg
    29761 gtgtagcaga acttataaat catgcagcat catttaaagc gttagagata cctactgatg
    29821 aagcaagtga gttgatattt ggtcatccat gtacttcaga agctttaatg gaagctttgg
    29881 cagatgtaaa tggagaatgt ttacacttac ctaaaaaata aatctaaaca taaattatta
    29941 atttaagtct attattaata ttaatttata tcataaaatt ttaaaacact tacttagcca
    30001 taccccttat tatgcaaata atttatttgc ataataaggg gtatttttta tggaaaaata
    30061 tagaatatag aatccttgta gtttttgttg tacaaaaaag atagtaatat aaaataacat
    30121 tatcatttta gaatgatttc aattgtaatt aggaaaagtg atttttttgt ttttaaaaaa
    30181 agtattattt gtaaaatata aaaaaaattt tcttaatatt tgaataaaag aatagcttaa
    30241 tttaaatgtt attactttat aaaaaaataa taagtacaaa aaaaacaata tatggcatat
    30301 aagttgctat ataaataata aggagggaac aattataaat gaggcaaaga ataaaaaact
    30361 taattgaaaa tgaagataag aaaaatcctt taacagatga agcgatttca agtaggttaa
    30421 atattcgaag agaagatgtt acctttttga gaaatgagtt aaaaatagaa gattcaagac
    30481 aaagaagaaa agttgtttta attaaagcta taaaagatat tttaaaagaa gaaaaaacta
    30541 taaataaaaa tgatataaca agaagattaa acagcattgg gtttacagta tcaagattta
    30601 cagtcataca atacttaaaa gagattcaag attctggtga cctaaaactt gataaagaga
    30661 gtgatgataa aaaagaagaa ataatcaata atagagaaaa taaagatata gcatttgata
    30721 aactcatagg tagtagtggt agcttaagta caattataaa actagccaaa gcagcaattc
    30781 tttatccacc tcatggtctt catactatta ttatggggcc aactggtgtt ggaaagagtg
    30841 agttagcaga gtgtatgtat aaatttgcag tagaatcatc cagattccct aagaatgcac
    30901 catttatagt ttttaatgca gcagattatt cagagaatcc acagcttttg ctatcacagt
    30961 tatttggaca cgttaaaggt gcttttacag gagctgatga gaacaaagaa ggattagtat
    31021 caaaggcaga tggaggaata ttgtttatag atgaaataca tagattacct catgaaggtc
    31081 aagaaatact atttcaactg attgataaag gtatggtcag aaagttagga gaaacaagac
    31141 taacacataa aatagatgta atgattattt cagcaacttc tgagccaata gattctcatt
    31201 tacttaatac atttaaaaga cgtatacctg taacaattga aataccagaa ttagttgcaa
    31261 gaccattaaa tgagaggttt gacattataa ataatttctt cttaatagaa gctcaaagaa
    31321 tgggagcaaa catacatata aagtcagatg tattaaaagc tcttatgctt tacgattgta
    31381 taggtaatgt aggtcaactt agaagtgata ttcaagtagc ttgtgctagg ggactattaa
    31441 accaacttac aaacaaacta aaagaagtaa atataagcat atcagatgta cctggatatg
    31501 taaaacaagg tcttataaaa atcagaaatt gtagaggaaa aatagaaaat tatgtagatg
    31561 gggatttaat tatagactca aatcttcaag gtgagataga aaagaaaaat gaagatatat
    31621 atacttttcc agatgaaata tacaaactta ctgaaagaag acatattgat ttattggaac
    31681 aaggtttaga ttttgatgtt ataaatagaa tcattggagg agaattagaa agttgcattc
    31741 aaaaatacat aaaacaagtt aaaaaagttt ctattagtaa caatcttcca gatataagtc
    31801 caattgttgg aaaatatatc gtagagctaa cttatgaagt aatcaaaata gcatcacaat
    31861 acctaagttc actagaaccg aatctacatt tatgtttagc agttcaccta aaagcagctt
    31921 ttgagagatt aaaagaagga aaagtaatat caaatgctca tttgaaagaa ataataggaa
    31981 attatccact agaatattct gtagcagaaa aggtagctca atatttaaag gaaagatata
    32041 acataaactt accaaaagaa gaaataggta taatagcaat atattttaaa atgagtagta
    32101 gaagagatat gtctgaacaa aaaaagatag gtgtacttct tatggctcac ggaagagtag
    32161 catcttctgt atgtgaggtt acaaataaac tattaggaat taatcacgca aggtatttag
    32221 atatgccatt tgagaagaaa ccggaagaaa tgattgaaga agctactaca ataataaaag
    32281 atattgatga aggtagaggt gtgattatat tagtagatat gggttcactt tcatattttg
    32341 gagatattat ttctggaaag actggaatta atataaggac aatatctaga tttgatacat
    32401 tgctttctat agaggttata cgaagagcag ttttaccaga tttaacaata gacgagatag
    32461 ttgatgaagt taaaggtata gagccacaag taaaagaaaa ggttagtaaa tgtaataata
    32521 caaaagtaga ttttagaaaa cctataattt taacattatg tattactgga ttagggggag
    32581 cttgtaagat aaaaaaaatc ctagaggaaa atattaaaag tataactgaa aaggttgata
    32641 tagaacctat aggtatgatt gaatatccaa gtcttgattt agagataaaa caaataagca
    32701 atgataaaaa tgtaatcgcc atagttggaa caattgaccc tcagatagca aatatccctt
    32761 tttttagtct agtagacata aatgataaag caaagttaaa tcaaataaat aacctaattg
    32821 atacaaatca aattggtgaa acaaataaaa aatatgaatt atcagattta atacataaag
    32881 aagatatatt tgtggattta caggttaaat caaaagaaga agttctaaaa tatatgagta
    32941 aaaaacttag agagaaaggt tatgtgacaa aaaatttcta taaaaaagta gtggaaagag
    33001 ataacttatt tccaactgaa ttatcagatg aaatagcaat acctcacaca gatggaatag
    33061 atgtaattag gccagctata agtatagcca tcttaaagaa gcctattgtg tggagtaaaa
    33121 gtaaagtaaa aataatactt ttattagctg tagaccaaaa gtgttttaat ccattaagca
    33181 ctctactctc atttatagaa acagatgaat ttaagaagat aaaaaacatt aaagatagag
    33241 attttattag ggagatgata ttagatggag tcaatgaaaa taattagtag caatttaatt
    33301 tttaaaaata ttgaagtgtc taacaatgaa gatgcattaa agtttttggg acaacgtttg
    33361 tttgacgaac agtatgtaaa agaaagctac atacaagctg ttgtagcaag agaaaaaaag
    33421 tatgctacag ggttaccaac agaaatatac ggagtggcaa taccacatac agatatagta
    33481 catgtaaatg agccaggaat agcaattgga atactgaata aacctgtaaa atttataatg
    33541 atgggtacag acgatactga aattgatgta aaagttgtat ttatgttagc agtgaaagag
    33601 ccacaagaac aattgcaact attagaaaaa cttatgacta tatttcaaga taagaatata
    33661 ctaaataata ttgttgactt aagtgaagaa agtgtgagtg atttattaaa ctctaaattg
    33721 aaaaattaaa catgtaaagt atattaaaaa tgtaaaatta aacatgcaaa gtatgttaaa
    33781 aaatataaaa agattattaa agatttctaa ctatagatat ctttaaaata aaataatttc
    33841 aatgggggtg actaatgtgg ctaaaaagat attagttgca tgtggaacag gagtatgtac
    33901 ttcaacaatt gctataaaca aattaaagaa agcacttcaa gacattggta aattggatat
    33961 ggttaatatt acacaatgta aagtagtaga agttgcatca aaagcacctg attatgattt
    34021 aataatttgt actacacaag tatctagtag tataaaaaca ccagtgataa atggattacc
    34081 ttttttaact ggtgtcggaa tggataaatt aatcaatgat gtattagaag agttagaatt
    34141 ataattaaaa aaattaagga acatggtctc taaaatcata agttttagag acttaaaatt
    34201 aaataatgga gggtctttat atggagatat taaatttcat tgttggtcta ggtgcacaag
    34261 taatgatgcc aataataata tgtatttttg gtttgatatt aggtacaaaa ctaggaaaat
    34321 ctttaagagc tggtttaact gtgggagttg ggtttatagg tcttaacact attatagctt
    34381 tgctaacaga taatttaggt ccagctacac aacaaatggt taagaattta gggctaagtt
    34441 tatcaataat agatgtagga tggccagcag catcagctat agcttttgca tcaacagtag
    34501 gggctttaat tattccaata ggtcttgtgg taaatatagt tatgctaatt accaatacta
    34561 ctcaaactgt tgatgtagac atatgggatt actggcattt tgcttttaca ggagctttag
    34621 tggcaggggc tactcaaagt gtaatgtggg gagtatttgc agcagtagca aatatggtaa
    34681 ttgtattggt aatggcagat ttaactgctc caggtataga ggaatattta ggtatgccag
    34741 gtatatctct tcctcatgga tttacacagg catttgttcc aattgctata gtagtaaata
    34801 aattattaga cttaatacca ggcataaata agattgagat aaatgctgat acattgcaga
    34861 aaaaatttgg attatttgga gaacctttaa taatggggtc tgtaataggt gtaataattg
    34921 gtattgctgc gaaatatgat ataaaaggaa tattacaatt aggtgtaact atgggagctg
    34981 tattaatatt aattccaaag atggcagcat tattaatgga aggattatta ccagtatcag
    35041 aagctgcaca ggaatttatt gagaagagat ttaaaaacag aggcaagatt tatataggct
    35101 tagattcagc agtaggaata ggtcatcctg ttacattatc agttgcacta gtgttggttc
    35161 ctttgactat acttatagca gctatattgc caggaaataa agttttacca tttgcagatt
    35221 tagcagtaat tccatttgca cttgtattaa tcgtaccaat tacaaaggga aatgtgttta
    35281 gaacattaat aataggaata attataatta cttctggatt attaatcgca actaacttag
    35341 ctccattatt tacccaaatg gctttaaatg ctagttttaa aatgcctgaa ggagcaacta
    35401 tgatttcaag tatttgtgat ggtgcgaatc cattaagttg ggtatttgta aaagttatga
    35461 actataaagt tattggagga gtagtttttg gagttatagc tttaggaatg gctatttaca
    35521 ataggaatag aataataaat gaaaataaaa agctatctat agaagaataa ggttaaaaca
    35581 attttattaa ggagtgtata ggttatgaaa aaatataaat atgattctaa aagatactta
    35641 ctcagtgtta caataccagg attttttatg attttaatat tgatttatgc actgtttaat
    35701 aactatacaa attttaattt gaatatatat agtttgttaa taattgtctg tacttatggt
    35761 atattcaata cttttatatc atcaagtaat cctaaaaaaa ttattattga tagcaaatgt
    35821 atacatttta cctcatttat gactacacat aagtatgaaa ttaaagatat agaaaagata
    35881 agaataagag aattttataa taaaaaaatt tatctaaaaa ttaatgatgg aaaacttttc
    35941 aaaggtaggt attggataag aacaaatatg tttaataata gtatagcatt atatagctat
    36001 ttcattgagt tggaagagtg cttatatcca gattcactaa aatttagaaa taaaaaattt
    36061 gagaataaga acatataaaa ataaaaaatt gaattgaagc ctagcttaat cttttttgat
    36121 aagatataag tttgaacttt atactaaatt tttagaattt tcaatcaaga caataacata
    36181 atagctaagc aattagaaca caactaagat gtataaggca tgtatatgat gaatgggagg
    36241 atttaattaa tgttattaga gaaattaaga aaagaagtat tacaagcatc tttagacctt
    36301 ttaaactata atttagtgac attaacagga ggaaatgtaa gtgggagaga tgaacaaact
    36361 ggctatatag caatcacccc aagtggaatg gattatagaa atcttacacc aagtgatatt
    36421 gttatagtag atgtagatgg aaatattata gatggaaaat ggaaagcatc agtggattta
    36481 tcagaccact tgtatatata taagcataga gaagacataa atagtataat tcatacacat
    36541 tctacatact caagttgttt tgctatttta aatgaaccta tagaatgtgc aagtacaact
    36601 cttgcaaatg aagtaggggg ttcagtacca gttgcaaaat tttcgccacc aacatctaaa
    36661 aagatgggaa aatgtgttat tgaagcaatt ggtgacaaaa gagcatgtct tttagcaaat
    36721 catggcgtaa ttgcagttgg tccatctgta ggacatgctc ttacagccgc tgtaatgtta
    36781 gaagattcag caaaagtata ttacttagca aaatcaattg gcacaccagt gctactgcct
    36841 gatgaagaaa ttcaaagagc gagagatgta ttttttaatg tctatggtca ggataaataa
    36901 attagataaa attttaaaag taatatgaaa atatagatat tcaatgatat taaggagatt
    36961 tataatgata taataagttt aatagttaag aagtaaaata tgaaatgtaa tagtatataa
    37021 attcatttaa gactaaaaag aataagataa aataaaaagg agtctgtatt tctatgtatt
    37081 acttattaaa caaaagcaca aatccttact ttaatttggc attagaagaa tatctatttt
    37141 taaatgataa atataatgat gatattataa tcatatggag aaatgaagaa tccattttta
    37201 taggtaaaaa tcaaaatcca tatcaggaag tatatcatga tgttattgaa aagggagaaa
    37261 ttcctatttt aagaagaata tctggaggag gaactgttta tcatgactta ggtaatatca
    37321 atatgtcatt catacaaaaa gataggcaac ttcatgagat agattttttg gagcatacta
    37381 agtttatgca aaagatgctt tcaaccttag gactagatgt atctataact gaaagaaaag
    37441 atttattttt aaatgggaaa aaaatttcag gctctgcaca gtctataaaa agaaaaaatt
    37501 ctctttatca tggaactctt ctttacgatt cagatttaaa taaattaact aaatatttaa
    37561 attcaaataa agcaactgaa tcaaatgcaa ctaaatctgt ttcaagtaaa gttacaaata
    37621 ttaagttttt actcgaaaaa gatataaatt actttttaga ttattgtgtt gaatatttaa
    37681 aatctaatat aaagaatata aaagaacttg aattggatga agaagatatt aaaagtgtat
    37741 acaatatagg agaagataaa tataaaaaga tagagtggac atatggtaaa acgccgaaat
    37801 ttcaagtagt gctaccattt aataatgaaa gtaaaataaa tatacatgtt aacagatgga
    37861 aagtatcaaa attttttatt tcttatgatg ataatgtaat taatatggac aagttgttag
    37921 atttgaattt ctttaaagaa gaaataaaag agagtatttt agaaaattat ccacattaca
    37981 tagatttaat caatttaata ttttaatata aaacctctaa tttgataaaa ttgtcttata
    38041 tcaaactaga ggtttttaat attcttgcat attaacaacc attacaaaat tgtaaaatta
    38101 aaaacaacac tattgaaaaa tcaatatata ttgtaataaa tatatgtgtt tttcattagc
    38161 aaatagacat taactattta tacaactgaa aatttaatat ataattaatc agatggttaa
    38221 aataaaaata ttaaaagttt taataaatga tggttaaaaa taaaatacga ggtgatacca
    38281 tgtatagtgt tataattgtt gcagcgggaa gcggaaggag aatgaactta gatataaaca
    38341 aacagttcat aaaattaaga gaaaaagaaa ttattgctca tacaattcaa gttttttatg
    38401 aaaatataaa tatagatgaa atagtggtat gtataaaaaa agaagaagaa gattttttta
    38461 aagaaaatat aataaataag tataacttta aaaatataaa aatagcatat ggcggaaaag
    38521 aaagacagga ctcaatatat aatggtttaa aaaaattaga taaaaattgt gatatagtac
    38581 ttatccatga tggagcaaga ccatttgtcg accatagaat aatcaatgaa tctataaagg
    38641 tagctaaaga aaaaaaggct gtggttgtag gtgttccagt aagtgatact ataaagatag
    38701 tgtcagatgg aacagtacaa gaaactccag aaagaaattt gctgtgggca gcacaaaccc
    38761 ctcaaacctt tgaatataat ctcataatag atgcttatga acaagcatat aagaacaatt
    38821 attatggtac tgatgattca atgttggtag aaaatatagg tcaaagtgtc actatggtaa
    38881 tgggttctta tgaaaacata aaaataacta gtccagaaga tttaaatata gcagaacaaa
    38941 tattaaatat ggaaaaaaga gatgaagtta acagtagaag gagaattata tgagaatagg
    39001 attaggatat gatgtacaca aattaacaga ggatagaaaa cttataatcg gtggtgtaga
    39061 gataccacac gataaaggtt tgttgggtca ttctgatgct gatgtattga tacatgcaat
    39121 aatggattct attttaggtg ctctagcatt aggagatata gggaagcatt tcccagatac
    39181 agatgaagaa tataagggag cagatagtat gaaattatta gaacatgtgt ataatctaat
    39241 aacaagtaaa ggatataaaa ttggtaatat agacagtact ataattgctc aaagccctaa
    39301 gatggctcca tatattgaga gtatgagaag taatatatca aaagttttaa acacagacat
    39361 tgataatata aacataaagg caacaacaga agaaggtctt ggttttacag gagcaaaaca
    39421 gggaatagct tcacaaagta tttgtttatt attgttgaca agtcagaata attaatatat
    39481 tattagtaat aagtttaatg gatatcaagt aataggccta tatagaatat ctataatgta
    39541 tgaatttaag tgaaattaat ataaatataa aattagctat gataaagaaa tagtaaaatg
    39601 atgaaatctt cacagagaga aaacggtggt gagagttttc aagaggaagt ctttgaacct
    39661 gtcttttagc ttaaaggtat aaaaatcttt acggtaggaa ccgttatatt cccaaaagag
    39721 agctattcat atagctaact agagtggtac cgcggaaatt aaacctttcg tctctatact
    39781 tgtatagaga tgagaggttt tttatatttt caggaagtta atattcagga ggaatctatt
    39841 aatgaaaatg tcaaaaatgt ttatgccgac attaaaagaa atcccagcag atgctgagat
    39901 tacaagccat caattaatgg ttaggtcagg catgataaaa aaaatgactt caggtgttta
    39961 taaccaatta ccaatggggt taagagtgtt caaaaaaatt gaacaaataa ttagagaaga
    40021 attaaataaa aaagattgtc aagaaatact ttgtgcagcc ttacttcctt ctgaattatg
    40081 gaaagagtct ggaaggtgga ctgctatggg tgaagaaatg tttagactaa aagacagaac
    40141 agaaagagag tactgtcttg ggccaacaca tgaagaagca ttcactgata taataagaca
    40201 agaaattact tcatataaac aattgccact gaacttgtac caaatacaag taaaatatag
    40261 agacgagaga agaccaagat ttggtgttat gagaactaag acttttacaa tgaaggatgc
    40321 ctatagtttt gatgtagatg ataaaggttt agataagtca tatcaagata tgtttgatgc
    40381 atatgtaagt atatttgata gatgtggatt ggagaatagc cctgtacagg cagattcagg
    40441 agctatagga ggttctacat cagccgaatt tatggttaag tcagaagttg gtgaagatga
    40501 agttgtattt tgtagtggat gtgattatgc agccaatgta gaaagagcag aatcttgtaa
    40561 tctagcatca caaaaagaag aaatgaaaga attagaagag gttcatacac caggagcagc
    40621 tacaataaaa gagttagaag aatttttaaa aacttctcca gataagtttg ctaaaacttt
    40681 agtatatgag gcagatggaa aaacagttgt agtcgttgta agaggagata gagaagttaa
    40741 tgaaatcaaa gtatctaatg ccataggttc tgttatagaa tttgctcttg ctacagatga
    40801 tgtagtgaga aaagtaacta atgcagaagt aggatttgca ggtccaatag gtataaatgc
    40861 tgactatgta tttatagata aagaaattgt tgaacaaaga aacatagttg ttggggctaa
    40921 taaaactgag taccatataa aaaatgctaa ctatggaaga gattttgaag gaatagttgg
    40981 agactttaga aatgttcaag aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat
    41041 agctagaggt gttgaagtag gacatatatt taaactagga actaagtact cagaatctat
    41101 gaatgcaaac ttcatagaca aagatggaaa atcaaagcct atagtaatgg gatgttatgg
    41161 aataggtgtt gaaagaacag cagcagctat aattgaacaa cataatgatg agaaaggtat
    41221 aatatggcct ttgtcagtag ctccatacca tgttgtgata gttccagcaa atatgaaaaa
    41281 tgaagaacaa atatcaatag ctgaaaatat atataatgat ttgcaagcta tgggtgtaga
    41341 agttttatta gatgacagag atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat
    41401 aggaatacct atgaggatta cagtaggtaa aaatattaat gaagggaaag tagaatttaa
    41461 acttagacat aaagaagata aagagataat tgacatagaa gaaataaatg aaaaagtaaa
    41521 ggcagagttt atcaggaata atgttaggtt aggataataa cttaaataag atattgtata
    41581 gataatcaaa gttgtacttt atagacacca ttttatctat atataaggat ataaaatggt
    41641 gtttttttat aaaacaaata aatgaggagg taattgaaat ggcaaaaaat gaaaaacaat
    41701 ttgttgaaga aataacaaag atggaagatg attttccaca atggtacaca gatgtaataa
    41761 ctaagactga cttagttgat tatgcaccag taaaaggttt tatggttgta aagccatatg
    41821 gatatgcatt atgggaaaag atgcaagaat ttatggataa aaaatttaaa gaaactggac
    41881 ataaaaactg ttattttcca ttattaatac cagagagttt attaaacaaa gaagcagaac
    41941 acgttgaagg ttttgctcca gaagttgcat gggtaacaca tggaggaaat aaaaaattag
    42001 aagaaagatt atgtgtaaga cctacttcag agactataat atgtacaatg tatgcgaaat
    42061 ggttaaaatc atatagagaa ttaccatatc tttataatca atggtgctca gttgttagat
    42121 gggaaaaatc aactagacct ttcttaagaa catctgagtt tttatggcaa gaaggtcata
    42181 cgttacatga aacagcagaa gaagcacaag aagaaacaat acaacaatta gaagtatata
    42241 aagcgctatg tgaagaatta cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg
    42301 agaaatttgc aggtggtgaa agaacatata ctatagaagc tatgatgcat gatggtaaag
    42361 cattacaatc aggtacaagt cacttcctag gtcaacactt tactaaggct tttgatatta
    42421 cttttgcaga tagggaagga aatttagcta atccatacca tacatcttgg ggagcatcta
    42481 caagacttat aggtggactt ataatgactc atagtgataa cagaggttta gtattaccac
    42541 caagagtagc tccaattcaa gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa
    42601 tggagactgt tgataaaata tatgctgatt taaaagctaa gggagtagct gtagaagttg
    42661 atgatagaga taactataca actggatgga aatttaatga gtgggaaatg aaaggtgttc
    42721 cagtaagagt tgaaattgga ccaaaagata tagaaaataa tcaagctatg gtatttagaa
    42781 gagatacttt agaaaaggat tctatgcctt tagaaggatt agctgatgct atatgtgatt
    42841 tatttgatgt tatacacaat gatatgtttg aaaaagcaag aaaacataga gaagacaata
    42901 catctatagt tgaaaatatg gatgagttta gaaaagcttt agaagaaaag ccaggattca
    42961 taaagactat gtggtgtgga gatgcagaat gtgaagctaa gattaaggaa gaaacaggag
    43021 caactataag atgcttacct tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg
    43081 gaaaagaagc agatagtatg gtagtaatgg caaaagctta ctaaaataca tttaaataaa
    43141 aactggggta gaaagtattt aatactttat cccagttttt tgttttttat gagtaataaa
    43201 atgtatttgt aactatgtca taagataagt catagatact tatattaaaa agaatataat
    43261 agagaactat ttttaataat tacatattaa tgaaagcaaa atattttgtt atatagattt
    43321 tcaataaagc aaacaataaa tataaatgaa tattagtaag gagtgctttt aatttttaga
    43381 aaataaatga cttaaaagaa agatttatga aattaaaact aggattttct gatataataa
    43441 acatgtgtat ttttaaataa aaaggatatt attattagat ttgattaaaa ataaacttgc
    43501 aaaacaatgt actagtttaa tattatagtt atattgaata aaatatcagg aggaagtaaa
    43561 aatgaatgta agagtaaggt ttgcaccaag tccaacagga tttgtacata taggtagttt
    43621 gagaactgct ttatataact atttatttgc aaaaaaaatg ggcggagaat atatattaag
    43681 agtggaagat acagaccaaa gtagattagt agaaggtgct atagagaata tgcttaatgc
    43741 tatgaaatgg gctggtgtca accatagtga aggtgttata ttagatgata gtggaaaagt
    43801 agtacaaaaa ggagaatatg gtccatatat tcaatctcaa agattagata tataccaaga
    43861 atatataaag caacttttag atagtggaaa agcttactac tgtttctgta caaaagaaag
    43921 actggatgaa gtaagagatg ctcaaagagc agcaggagaa acagctaaat atgatggtca
    43981 ttgtaagaat ctttctaaag aagaagtaga agcgaatata aaagcaggaa taccatatgt
    44041 tataagatta agattgccag agaatcatac aattaagttt acagacttag ttagaggaga
    44101 tatggaattt aatactaatg acttagatga ccaagtatta atgaaaacag atggatttcc
    44161 aacttatcat tttgcagtag ttgttgatga ctatttgatg aaaattacac atgttataag
    44221 aggagaagaa tgggtatcat ctactccaaa acatgtttat ttatatgaag cttttggatg
    44281 ggaagcacct gtatttgtgc atttaccaaa tatacttaat aaagaaaaga aaaaattaag
    44341 taaaagacaa ggtgatgtag ctgttgaaga ctttaagaaa aaaggatatt taccagaggg
    44401 attagtaaac tatgtagcat tagttggttg gtctcctgaa gataatcaag aattgtttac
    44461 aatggaggaa ttagaaaaag cgttctctgt agaaagagta tctaaaagtg gtggagtatt
    44521 tgatactgaa aaattaaatt gggttaatca gcactatata aaagatggtg atgatgctta
    44581 tctaacagac ttagcaatac catttttaat agaagatgga tttataacag aagaagaagc
    44641 tactaataaa tatgatttct taaagagtat gatatcagta cttaaagaga aattacaata
    44701 tgtaaaagaa gttactgaac atgcaagtat attttttgga gataaaatag aggtagaaac
    44761 tgaagaggga agagagttct taagattaga acatatacct actttaatag atgctttgag
    44821 agaaaaaata gaaaaaacag aggtattaaa tgctgagttt gttcaagcta tgcttaaaga
    44881 aatacaaaaa gagtacaaaa taaaagggaa aaatttattt atgggctcaa gaataatttt
    44941 gacagggcaa atgcatggtc cagatttacc aaaagtaatg gaagttttag gaaaagaaac
    45001 ttgcttaaat agaatagctt atgtaaaaaa taatatatta tagtcataaa agtccgctaa
    45061 tttagaagcg gacttttttt gtgttagtat ttggtgaaaa tatctgctta agaaaatgaa
    45121 ggtatttaag gtaataaatt taatatataa aaagcaaagc aaaaatataa ataaaatatg
    45181 aataataaac taataaaata ttaagcatag aagaagggaa tctattattt tagtttgatt
    45241 taattattaa gggggtaatt actatgtata gtaatattga tgatgttaaa aaggaattaa
    45301 aggagctatg cttagaatat gttacaattt tagaaaaatt aaaagatgaa aaaatgatta
    45361 cagaagaaac ttttgaaaag tgctcttctc aaaaaaaaat atttttagaa gaacaataga
    45421 ggatggtaat agtattatta ttatgtagtt tgaaagatag tacgaaaaat gatagtaata
    45481 ctattattta ttgcaatttt caactataat ataatatata atgttaagaa gataatttat
    45541 aaaaatacag tgaggtgatg agtgtgaaag tttataatac attgactaga acaaaagaag
    45601 aatttgttcc attagaagaa ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata
    45661 actatataca cataggtaat gcaagaccat ttataatatt tgatactcta agaaggtatt
    45721 tagagtatag aggatatgat gtaacttatg tacaaaactt tacagatgtt gatgataaga
    45781 ttataaatag aagtcatgag gagggaatat ctcctgaaga agttgcggct aagtatataa
    45841 aagaatattt tgtagactgt gatgggctag gaataaaaag agctactgtt catcctcagg
    45901 ttacagataa tatacaacag attatagagt ttataaaaga gttggaagat aagggatatg
    45961 catatgcagt aaatggtgat gtatattttg atacaaataa gtttgaaggt tatggaaaac
    46021 tttcaggtca aaaacaagaa gatttagagg caggggctag aattgaggtt aatgaccaaa
    46081 aaagacatcc tatggacttt gttctttgga aggcaaaaaa agaaggtgag ccaggatggg
    46141 atagcccttg gggagaaggt agaccaggat ggcatataga atgttctgtt atgtctaaga
    46201 gatatttagg tgaaacaatt gatatacatg caggtgggca agatttaaca ttcccacatc
    46261 atgaaaatga aatagctcaa agtgaagcta gaagtggaaa aacattctca aaatactgga
    46321 tgcataatgg atatataaat ataaatgatg aaaagatgag taaatcaaaa ggtaatttct
    46381 ttacagtaag agatatatca aaattatatg atttagagat agtaagattt ttcatgttat
    46441 cagcacatta tagaaatcca gtaaacttta gtgatgagat gttaaatcaa gctaaagcag
    46501 gtcttgaaag actatataat actaaagaaa aattagaatt tacgctaagt aatttagtag
    46561 aatctccgtt aacagaaaaa gaagtagagc ttgttaaaga attagatgac tttagacaaa
    46621 aattcataga tgctatggat gatgatgtta atacagcaga cgcagtaagt gtaatatttg
    46681 aattagctaa gttaataaat tctaatgtag atgaaaattc ttctttggag tttgctaaaa
    46741 aatgcttaga tgaatttaat gaacttacag gagtattaaa tattgtaaat aagaaaaagg
    46801 atactgtatt agataaagac attgaagaat tgatacaaaa aagaactgat gcaaagaaaa
    46861 ataaagagtt tcaattagct gatgatatta gacaacaatt actagatatg ggaattgtat
    46921 tagaagacac aagacaaggt gttaagtgga aaaggatata aaaatggaaa aaactgaatt
    46981 agttactatg tcaccattag ttttagcata cttaggtgat acagtgtatg agacatatat
    47041 aagagaatat ttaattagac agaatacaca aagaaaagtt aatgatttgc ataaactagc
    47101 tataaagtat gtaaaagcta aagctcaggc aactataatt catgaaattg aaattgagtt
    47161 aactgaagaa gagagtaaaa tttataaaag gggaagaaat caaaagtcaa atacttctcc
    47221 taagaatgca gatattatag actacaaaca tgcaactggg tttgaggcat tagttggata
    47281 tttgtattta aataatgaaa tagagagact tcaatatatt atcaataaag gaatcaaaat
    47341 tattgaaaga gatatgtaaa tattaaagag tgttgtctta ataataagta ataaggcaac
    47401 acttttttta taactaatgt ataataaata tatagaaatt agtaatgaga aaggataaaa
    47461 agttatgaaa gtaaaattaa tatcacatac acctgaacca gaaaaagtaa tagctatggc
    47521 agctaagcta tgttattcac cagttgggac agacgaaata gaaaaagact taacagatga
    47581 aagtatagag aaatttttaa atatgttatt aagtataggt catgggtcaa tattagaaca
    47641 tgcttcattt acattttcaa tagaaggtat ttcaagggca tgttcacatc aaattgttag
    47701 acatcgtata gctagttttt cacaacaaag tcaaagatat gttaaattag aacaatttga
    47761 gtacataatt cctccagaaa tagaaaaaat agaaaaggca aaagagttat ttatagactc
    47821 catgaaaaaa gaccaagaga attatgataa gttagttgaa atattgtttg aaaatcatta
    47881 taatgaccta ataaaaaacg gtaaaaatga gaaaacagct aaaagacaag ctgagaagaa
    47941 agcaatagaa gatgctagat acgtatttcc aaatgcatgt gaaacaaaaa tggtatttac
    48001 tataaatgct agaagtttgt ttaatttctt tgaacataga tgctgtgaaa gagcacagtg
    48061 ggaaataaga aatttagctg tggagatgtt aagagaggtt aaaaaggttg ctcctatatt
    48121 atttaaaaag actggtccaa gttgtgtaaa tggaagttgt ccagaaggaa ctatgacttg
    48181 tggagatata gttcaagtta gagaaaaatt taaggccttg taggaggtga ataatttggc
    48241 gagtatagaa ggaagaaatc cggtaataga agcaataaaa agcgacagag aaatagataa
    48301 aatattaatt gcaaattcag ctaaagaagg ttcaattaag aaaataatag gaatggctaa
    48361 agataagaat ataattattc agtatgttga taaacataag ctagatgaag taagtacaag
    48421 tcattcacat caaggtgtaa tagcttatgc aagtgagtat aagtattatg aattagatga
    48481 gctaatagat ttagcaaaga ataaagatga agacccattt ttcattatat tagatgagat
    48541 aacagaccca cataatttgg gttcaataat aaggacagca gatgcagtag gagcacatgg
    48601 agttatcatt ccaaaaagaa ggtctgtaca cataactcct gtagttgcca aagcatctgc
    48661 tggagcagta gaatatatgc cagtttgcaa ggtaaccaat atagttaata caataaaaag
    48721 actcaaagaa gaagggctgt ggattgctgc agctgatatg gatggagaaa ctttttataa
    48781 acaaaatctt actggtcctc ttggtgtagt aattggaagt gaaggttttg gaatatctag
    48841 attggttaaa caaaactgtg actttatagt aaaaatgcca atgataggta atgttacatc
    48901 tttaaatgca tctgtagcag gtggaatact tttatatgag atattcagac aaagactgga
    48961 taaaagtaaa taggcatgaa aaaaaatata aatcattact taataataga tggctacaat
    49021 attataaatg cgtgggacaa tttgaaagaa ctggctaaag aggacttaga agattctcga
    49081 gaaaagctta ttgatgacat tatagaattt tctgagttta tgggttataa gactataata
    49141 gtttttgatg cttataatgt taaaaattct cgagaaaaag ttgaaaaaag aaaacacata
    49201 acaatagttt atacaagaga acaccaaaca gcagatagtt atatagagaa atttataact
    49261 tctttatcaa agtatgatga tgtaaaagta gccactaatg actatgcaga acagcaaatg
    49321 attttaggaa aaggtgctac tagaatgtct gcaagagaat taaagttaga gttagatcgt
    49381 tccaagaata aaatgaagga aaaaaatatc ggtttgagga aaaaaattca gcgaaattgg
    49441 ttggaggaaa ggttagataa agaaacattg tcgaaacttg agaacattcg tagaaagcgt
    49501 tgaaattact aggttcttta gactataata tacctataaa tttgttttat atatggggga
    49561 gatttacatg ttggtagcaa aagaaaaaag ttatgagtta gtggacaatt gtcagcagga
    49621 tgagtataac attgtattaa gagcaagtga aggggataaa atagcactag agtacattat
    49681 tactaaatac agaaattttg taaaggcaaa agcaaagtca tattttttaa ttggagcaga
    49741 taaagaagat ataatacaag aaggaatgat aggcctttat aaggcagtta gagattttga
    49801 tggaagtaag accaattcat ttaaatgttt tgcggagata tgtataacaa gacaaataat
    49861 aactgctata aaaactgcaa caagacaaaa acatatacct ttaaattcat atgtttcttt
    49921 gaataaacca atatatgatg aagagtcaga cagaacactt ttagatatta tagctacaag
    49981 tatagtaaca gatccagaag agcttataat tagtaaagaa gagttaaaaa atatagaatc
    50041 aaaaatgaac gaattgttaa gtgacttgga attagaagta ctagaattat atttaaatgg
    50101 aaagtcatac cagtttatag cggacaagct taaaagagat gtaaagtcaa tagataacgc
    50161 tttacaaagg gttaagagaa aattagaaaa acatcttgaa aacagaaatg attaatagta
    50221 aaatacttac cataagctta ttgtgagcaa aattaattta tatcgaaaga taagggagga
    50281 aattcaaaat ggctaaagct aaatacgaaa gaacaaaacc tcatgttaat atagggacaa
    50341 taggacacgt agaccacggt aaaactacat taacagcagc aataacaaaa acattatatg
    50401 acagatatca attaggagaa gcagtagatt tcgcaaacat agataaagct ccagaagaaa
    50461 gagaaagagg aatcacaata tcaacagcac acgttgagta tgaaacacca a
//
LOCUS       DAENCV010000013        49614 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAENCV010000013 DAENCV010000000
VERSION     DAENCV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.013"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..399
                     /locus_tag="KQD82_000693"
     CDS             <1..399
                     /locus_tag="KQD82_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG4379183.1"
                     /translation="TISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVGVIMYLSIAS
                     LIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKFYIVVIYYIGVF
                     FYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            353..1396
                     /gene="holA"
                     /locus_tag="KQD82_000694"
     CDS             353..1396
                     /gene="holA"
                     /locus_tag="KQD82_000694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG4379184.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1583..1849)
                     /gene="rpsT"
                     /locus_tag="KQD82_000695"
     CDS             complement(1583..1849)
                     /gene="rpsT"
                     /locus_tag="KQD82_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG4379185.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            2448..2969
                     /locus_tag="KQD82_000696"
     CDS             2448..2969
                     /locus_tag="KQD82_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379186.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            3003..3779
                     /locus_tag="KQD82_000697"
     CDS             3003..3779
                     /locus_tag="KQD82_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4379187.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            4122..5075
                     /locus_tag="KQD82_000698"
     CDS             4122..5075
                     /locus_tag="KQD82_000698"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG4379188.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            5093..6112
                     /locus_tag="KQD82_000699"
     CDS             5093..6112
                     /locus_tag="KQD82_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG4379189.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            6143..6394
                     /locus_tag="KQD82_000700"
     CDS             6143..6394
                     /locus_tag="KQD82_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379190.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            6676..8481
                     /gene="lepA"
                     /locus_tag="KQD82_000701"
     CDS             6676..8481
                     /gene="lepA"
                     /locus_tag="KQD82_000701"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG4379191.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            8630..9967
                     /locus_tag="KQD82_000702"
     CDS             8630..9967
                     /locus_tag="KQD82_000702"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG4379192.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIHCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            10200..11513
                     /locus_tag="KQD82_000703"
     CDS             10200..11513
                     /locus_tag="KQD82_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4379193.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            11685..12860
                     /locus_tag="KQD82_000704"
     CDS             11685..12860
                     /locus_tag="KQD82_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG4379194.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            12963..13982
                     /gene="hrcA"
                     /locus_tag="KQD82_000705"
     CDS             12963..13982
                     /gene="hrcA"
                     /locus_tag="KQD82_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG4379195.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            14012..14632
                     /gene="grpE"
                     /locus_tag="KQD82_000706"
     CDS             14012..14632
                     /gene="grpE"
                     /locus_tag="KQD82_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG4379196.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            14724..16571
                     /gene="dnaK"
                     /locus_tag="KQD82_000707"
     CDS             14724..16571
                     /gene="dnaK"
                     /locus_tag="KQD82_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG4379197.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            16782..17936
                     /gene="dnaJ"
                     /locus_tag="KQD82_000708"
     CDS             16782..17936
                     /gene="dnaJ"
                     /locus_tag="KQD82_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG4379198.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            18902..19837
                     /locus_tag="KQD82_000709"
     CDS             18902..19837
                     /locus_tag="KQD82_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG4379199.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            19873..21246
                     /locus_tag="KQD82_000710"
     CDS             19873..21246
                     /locus_tag="KQD82_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4379200.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLANAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(21434..22201)
                     /locus_tag="KQD82_000711"
     CDS             complement(21434..22201)
                     /locus_tag="KQD82_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379201.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(22231..23340)
                     /gene="ugpC"
                     /locus_tag="KQD82_000712"
     CDS             complement(22231..23340)
                     /gene="ugpC"
                     /locus_tag="KQD82_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG4379202.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            23598..24335
                     /gene="cas6"
                     /locus_tag="KQD82_000713"
     CDS             23598..24335
                     /gene="cas6"
                     /locus_tag="KQD82_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG4379203.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESVRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            24340..25815
                     /locus_tag="KQD82_000714"
     CDS             24340..25815
                     /locus_tag="KQD82_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379204.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            25835..26848
                     /locus_tag="KQD82_000715"
     CDS             25835..26848
                     /locus_tag="KQD82_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG4379205.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            26864..27661
                     /gene="cas5"
                     /locus_tag="KQD82_000716"
     CDS             26864..27661
                     /gene="cas5"
                     /locus_tag="KQD82_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG4379206.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVALKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            27688..30096
                     /gene="cas3"
                     /locus_tag="KQD82_000717"
     CDS             27688..30096
                     /gene="cas3"
                     /locus_tag="KQD82_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBG4379207.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNI
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKNILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQKEIYDYIE
                     KGILEKEDFNLEDIEDYIKEILYVDFKFNEDVLDYDYIDKLSESMVIYSDYNNGQIED
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            30538..31485
                     /gene="prmA"
                     /locus_tag="KQD82_000718"
     CDS             30538..31485
                     /gene="prmA"
                     /locus_tag="KQD82_000718"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG4379208.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            31504..32262
                     /locus_tag="KQD82_000719"
     CDS             31504..32262
                     /locus_tag="KQD82_000719"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG4379209.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            32264..33562
                     /gene="mtaB"
                     /locus_tag="KQD82_000720"
     CDS             32264..33562
                     /gene="mtaB"
                     /locus_tag="KQD82_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG4379210.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            33605..33955
                     /locus_tag="KQD82_000721"
     CDS             33605..33955
                     /locus_tag="KQD82_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG4379211.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            34118..34297
                     /locus_tag="KQD82_000722"
     CDS             34118..34297
                     /locus_tag="KQD82_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG4379212.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            34327..34770
                     /locus_tag="KQD82_000723"
     CDS             34327..34770
                     /locus_tag="KQD82_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG4379213.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            34881..35378
                     /locus_tag="KQD82_000724"
     CDS             34881..35378
                     /locus_tag="KQD82_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG4379214.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            35571..36134
                     /gene="raiA"
                     /locus_tag="KQD82_000725"
     CDS             35571..36134
                     /gene="raiA"
                     /locus_tag="KQD82_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG4379215.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            36347..36583
                     /locus_tag="KQD82_000726"
     CDS             36347..36583
                     /locus_tag="KQD82_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG4379216.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            36596..37939
                     /gene="yqfD"
                     /locus_tag="KQD82_000727"
     CDS             36596..37939
                     /gene="yqfD"
                     /locus_tag="KQD82_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG4379217.1"
                     /translation="MISFIRGYYVIVVEGVRLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKESYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            37878..38993
                     /locus_tag="KQD82_000728"
     CDS             37878..38993
                     /locus_tag="KQD82_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG4379218.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            39050..39511
                     /gene="ybeY"
                     /locus_tag="KQD82_000729"
     CDS             39050..39511
                     /gene="ybeY"
                     /locus_tag="KQD82_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG4379219.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            39517..40236
                     /locus_tag="KQD82_000730"
     CDS             39517..40236
                     /locus_tag="KQD82_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG4379220.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            40355..40756
                     /locus_tag="KQD82_000731"
     CDS             40355..40756
                     /locus_tag="KQD82_000731"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG4379221.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            40856..41749
                     /gene="era"
                     /locus_tag="KQD82_000732"
     CDS             40856..41749
                     /gene="era"
                     /locus_tag="KQD82_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG4379222.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCEKDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            41761..43140
                     /gene="mgtE"
                     /locus_tag="KQD82_000733"
     CDS             41761..43140
                     /gene="mgtE"
                     /locus_tag="KQD82_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG4379223.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            43213..43368
                     /locus_tag="KQD82_000734"
     CDS             43213..43368
                     /locus_tag="KQD82_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG4379224.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            43334..44107
                     /gene="recO"
                     /locus_tag="KQD82_000735"
     CDS             43334..44107
                     /gene="recO"
                     /locus_tag="KQD82_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG4379225.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            44100..44699
                     /locus_tag="KQD82_000736"
     CDS             44100..44699
                     /locus_tag="KQD82_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG4379226.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            44859..45737
                     /gene="glyQ"
                     /locus_tag="KQD82_000737"
     CDS             44859..45737
                     /gene="glyQ"
                     /locus_tag="KQD82_000737"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG4379227.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            45737..47803
                     /locus_tag="KQD82_000738"
     CDS             45737..47803
                     /locus_tag="KQD82_000738"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG4379228.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMSFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(47969..49516)
                     /locus_tag="KQD82_000739"
     CDS             complement(47969..49516)
                     /locus_tag="KQD82_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG4379229.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        19890 a         5182 c         8755 g        15787 t
ORIGIN      
        1 acaatatcat caaatatatt aactttacct ataatttatt atacatttaa aggtatacca
       61 ttgctttcaa ttataggaaa tttaattata gtaccatttg tgggagtaat aatgtattta
      121 agtattgcaa gtttaatcgt atttaaagta agtgtagtaa tagctaaaat tatatctttt
      181 tttaatagta cattgataga aagcatattt tttttactag aaaaaatcag caatttaagt
      241 tttgcataca ttaatataga aaatcctaaa ttttatattg ttgttattta ttacatagga
      301 gtgttttttt acatatttta catagaagga aaagagataa aggagcaaga gaatgaatta
      361 caaggatatt ataaggaatg taaaagagaa aaactttgaa aaaatgtatc ttttttatgg
      421 aagagagtat tatttaattg agaatgcaat aaaagcattt aaagatagtc taaacgaggg
      481 aatgcttgat tttaatttag atataataga tgggaaggaa atagtcttaa atcagcttat
      541 aagctctata gaaacacttc catttatgga tgataggaag atagtaataa taaaagactt
      601 tgaactatta aaaggaaaga aaaagaattt tacagatagt gatgagaaat acttaataga
      661 gcatttagac aatattccag acaccacaac tatagttttt gttgtatatg gagatgtaga
      721 caaaagaaaa tcattagtta agaaaatagg aaacaatgga attgtttttg attgtgataa
      781 gctttctgat atggatttgt ttaaatggat aaaaaaatct tttgctttaa atgatgttat
      841 aatagataat tctcaaatca tgtattttat tgaacaagaa ggatacagag ataagagtag
      901 tgaaaaaaca ttatctgatt tagaaaatga aataaataaa ataagttcat ttgttggtaa
      961 agggaataat gtaaccaatg atgtaattga taaattatct caaaaaaaag ttgagaatga
     1021 tatattcaaa ttaatcgatt atattggaga acaaaatgct tctaatgcaa tgaaaatatt
     1081 aaatgatatg atacaagaag gcgaatctgt attaggtata ttttctatga tagcaagaca
     1141 atttaaaata attatgcaag taagacagtt acaacttgat ggatattcaa caaaacttat
     1201 agctgataaa ttaaaaatgc atcaatttgt tgtaggaaaa gcattaaagc aaactaaaaa
     1261 tttttctgat gatataattg tggaaatatt aaactatata ttagaaagtg attacaaaat
     1321 aaagacaggt cttataagag atactttagc agttgaaatg ttggttagta gatactgtaa
     1381 aagagaagca atttaaaaaa tctatatatt aataaaaaat aaatagcaag atatgttata
     1441 gttcagagta gatgtaagct aaattaagtt tgatatacaa tcaactataa tatgtctaac
     1501 tatttatttt ttattgtata aatttacaaa ataaaaaacc cctacatagg ggttattata
     1561 tataagaaat ataaactggt aattaagcgt tcataccgtt taatttttga gctaatttag
     1621 ctacttttct agctgcagca ttcttatgta tagttccttt agaagctact tggtatattc
     1681 ttttttgagc gtattggaac ttagcaacag catcctctct gtttccagct gcaacagcat
     1741 cttcgaatct tcttattgca gtttttattt gagattttct aactctattt aaagcagttt
     1801 tcttttcgat aacacttatt cttttcttag ctgattttat atttgccacc gtgttcacct
     1861 ccctgtattt ttttatcata ttcatcgtca acatattaga ttttaacaga aataaatcta
     1921 aaaatcaagg attattattt atatatttta ataatgtttt ttattttgat tttttagact
     1981 gtaaattgtg tataaataat ttgcaatatc ttgtttttaa attggtatat tttgtgtttt
     2041 aaatattttg acatatataa attaatattt tatttttgaa acaaatattt tgagataata
     2101 caaactatta acatacattt tgatagtatt tccaataatt ataagataat acaagaaatt
     2161 actcattttt gtaaatttaa tgaaaaatta taaaaatata aatttatatg cttaattaag
     2221 ataatttttc caataatcaa gataattttt ttcattagta tgtatgatag atgcatcaaa
     2281 caatataact aatatgttag aaggttatat gtaattaatc tcagtgttta tatagaatag
     2341 catttgtagc atttattata gttgtaatta ctacttatat catagacttg aatagtctaa
     2401 aaaaatagac tataatattt ttatagacta aagaaagggc tgataagatg gacatagcaa
     2461 aactaatttt aaatcctgca aggcttcgta tcttacaata tattcgactt catggcagtg
     2521 ttcgtacatc tgatattgta aagtatttaa atgatattcc tcgtgcaaca gtttatcacc
     2581 atgtaaaaat tttagaagag aacaatatga ttgaagtaat taaagaaaat cgtgtgcgtg
     2641 gtactattga aaaggtttat gcactaaagg aatatacaac atctatggaa ggagagacct
     2701 tcgttgcact ttctacagca ttttatgtag gtctgatgca ggaaatgaat gaatacttta
     2761 gcagaaaaga gcaagaccat aaaaaggata atgtattttt ctcttcagca ttactatata
     2821 tgagtgataa tgaatatgaa aatcttctta aaagtatagc tgatttattg aaaccatata
     2881 tagaacaaaa gcctaaatca aatctaaaac ttagaaaact ttcaattata tcttctccac
     2941 ctgtagctga taatgataaa acaaaataac atgcaaccca tttttataaa aggaggttat
     3001 atatgaaatt tcatgagttt ggaaataaaa acagaccaca tattatgttg attcatgggg
     3061 gagggaatgc atggtggaac tatttgcgac aagcagaagt tttgtctaac aaatatcatg
     3121 tgatacttcc tacattggat ggtcatggag aagaatatga aacaacatac atttctacag
     3181 aggatacagc agataagttg atatcatata tagatgaaaa ttgcagtgga catcttttcg
     3241 ctttgtgtgg ggtatcactt ggtggtcaaa ttgtcatgga acttttatct cgaaaaccta
     3301 atattactaa aaaagcaatt attgatggta gtatttgcta cccaagacct cttatggcaa
     3361 ggttttgtat tgctggtatt cgattttttg gaggactttt gtttagtgag aaggcttgtc
     3421 gctttcaaat tgctatgatg ccaaaattgt tacctgaaaa tatgcaatat ccagatgaaa
     3481 taaaagcata ttatatgaca gatatgccac atgtaagaaa agaaacattc tataatatgt
     3541 atcgtactta tatgatgaac tatacactta aagagaatgt taaaaaaacc acagcacaag
     3601 taatgtactg gtatggagaa aaagaaatga agtgtgtcaa aaaatctgca aaactgtttc
     3661 agtcttatgt gccctcttgt gagatttatg aagctaagag ctataatcat gggtatttat
     3721 cactttatct tccaaatgaa tggttggaaa ttgcagaacc atttttccaa aaggaataaa
     3781 ggtgttaggt gattattgta taattattgc tgttttttaa tatagtaaat aaattaggta
     3841 taaatataaa aaagttattt tagttaatat attagtgaaa gcttaatagg aaaattataa
     3901 atagattaaa ggtagcgcct atataaatag ttgctacctt tttctgtttg taaataaatg
     3961 atagataaga aggtttactg acaaatcact gactaaaccc aattttctta cattgaaagc
     4021 cttaatttta cttgttttaa ataaatttac aatttcttgt tataaaatac taaattttgt
     4081 taacaattat aattagcatt taatttatac tggaggttta tatgattagt gtaagaacag
     4141 atttagcttt agaagctagt gaaatgtgtg aaaagtctca agaaggaagt agtattcctg
     4201 gtgtaaaaat agaaactaaa gaattagaaa attgcattgt aactaaagtt gaagtaatag
     4261 acaggcaagg cgcagagata atgaataaag atataggaaa gtatataact ctggaaagta
     4321 atcttatgaa gtttgatgat gatgagtcaa gagaagaaat gataagttat ttaaaagagg
     4381 agttagtaga tatatttggc caagacaaaa ataaaaaaac cctggtaatt ggtcttggaa
     4441 atagaaatat tacttctgat gcgttaggac caaagagtgt atcaaaaaca cttgtaacta
     4501 gacatctttt taaaaattat aataaagact atgatgatga tttcactgag gtatcagctt
     4561 taagcccagg agttatgggt gttactggaa tagaaactag tgaaatagta aaatcattgg
     4621 ttgagaaggt taagccagat agagtagtag caatagatgc attggcttca agaaaaatgg
     4681 agagagtaaa ctctactata cagatatcaa cagcaggaat atcacctggt ggaggtgttg
     4741 gcaatactag aaaatctcta actaaagaaa ctttaggcgt ggatgttatt gcaataggcg
     4801 ttccaacggt agttgatgca gcaactctta caatagatgt cttagatatg gcaatagaca
     4861 atttaatagc tcaatcagaa gaaacagaaa gtttttatga aatgttgaaa aaacttaaag
     4921 aagaagaaaa atatcatcta ataaaagatt cgttagaccc atatgacaag aacttaatag
     4981 taacaccaaa ggatatagat gatacaatcg aaaatctatc tataataata agtgaaggct
     5041 taaataggtc tttacatcca ggtagattgg tataaaataa ggaggagcaa ttatgtttaa
     5101 aaaatgcatt aaagtagtaa ccttgacttt tatattagta tgcattttac caggaaaatc
     5161 attagctttg aatcaagatg attttttaaa gtttttagta aattcatctt atccagaagc
     5221 taaagtcgaa ggaaatgata cagaaaataa aaagaataat aaaaataaag aaacaagtaa
     5281 ggaaaataag gaagaaagta aagaagaaaa cactaaatct aaagatgcaa gtaaagtcga
     5341 taataaaaaa gagagtgaaa aagaatatat aaaactttat gttggtaaag aaaatgttcc
     5401 agatattgag tcaaaaaatt cagacacaac tgaaactaac acaacatcta gttcagatta
     5461 taaggatgat ttaagagtaa ccaaagaaaa tccaagaatt ttgatatatc atactcatgg
     5521 atgtgagact tattcaaatt cacctgatgg aaattatcat tcaagagata agaaaaattc
     5581 agtaatggaa gtaggaagtg cattaactag tgctttagat agcaaaggat ggggtgtagt
     5641 ccatactact aaataccatg actatccatc ttataataac tcttatgcaa gtagcttaaa
     5701 aacaatacaa agtatattac ctaaatataa ctcagtggat atagcgatag acttacatag
     5761 agatgctagg gatttaacta atccagccac taaggaaaaa gaccatttaa aatatacaac
     5821 tatgataaat ggagagagag tatcaaagtt cttctttgtt gtaggaggta aaaatacaaa
     5881 tagaaaacaa cttagagctt tagcagaaga tataactgct tttgctgaga aaaagtatcc
     5941 tggtctagta tctccaatag tagaaaaaga ctatgctaga ttcaatcagt ttgcagttaa
     6001 gaatcatatg ttagtagaaa tagggaataa cgcaacaagt gtagaagaat ctaaggctac
     6061 tacaaagtat ttagccgaga ttttggatga atattttaaa caaaaaaatt agagctttaa
     6121 gttataatta ggagcatgct aaatgagccg attagaaaaa aatatagaaa gtaaaagtaa
     6181 actaagaaaa tacaagtata ttatgagaat cttatttttg attactatga ttatatgtac
     6241 atcagtttgt gtatttatag tggacagtaa tgcaaaaagt atgctaggaa tgaaacaata
     6301 tgattttcca agtattaaat ttgatataac aaaatttgat tttgacatac caaattttga
     6361 tatacatgaa atcataaaaa aatttaataa gtagtaataa gtgaataagg tttattgatt
     6421 aaaataagcc ttattttagt tatatatttt taatgtataa aataaaaatt cattaactgt
     6481 attttatagg atttacttta agtttacgat attatataat atttagtgtt agacttgata
     6541 atccacatat aatgtggata attttgttta gtgccaatta gtttatattc aaaaatttta
     6601 aatttggtat ataaagagtt tttagataat gtaaaataat aactataaga ataatcaaga
     6661 ggagggaatt ttaaggtgga caataaacaa agtagaacta gaaattttag tataattgca
     6721 catatagacc atggaaagtc taccttagct gatagattaa tacaacaaac tgggcttgtt
     6781 agtgaaagag acatgaaaag tcaattactt gacaatatgg accttgaaag agaaagagga
     6841 atcactataa agcttcaaaa cataagatta atgtataaag cgaaggatgg aaatgaatat
     6901 tatttaaatc ttatagatac acctggacat gtagacttta actatgaggt atcaagaagt
     6961 ttagctgcat gtgaaggagc actattagta gttgatgcag ctcaaggtgt tgaggcacaa
     7021 actttagcca atgtttatct tgctatagac caagatttag aaatattacc aataataaat
     7081 aaaatagatt taccaagtgc aagaccagag gaagtaaaaa atgaaataga agatttaata
     7141 gggcttgatt caagtgaagc tcctcttatt tcagctaaaa ctggtttaaa catagaagat
     7201 gttttagaag atattgtaaa aaatgttcct ccacctaaag gagataatga agctccatta
     7261 aaggcattaa tattcgattc ttactatgat gcttacaaag gtgttgtagc ttatgttaga
     7321 gtatttgaag gaactgttaa aaagggtatg acaataaaaa tgatgaatac caataaaaag
     7381 tttgaggtaa ctgaagttgg tgtcatggct ccaggtcaaa ctgaacttag tgaattatca
     7441 gcaggtgatg ttggttatat tgctgctagt ataaaagata taagaagttg tcgtgttggt
     7501 gatacaataa cagattcaaa taatcctaca gaagaacctt taccaggata caaaaaagct
     7561 actccaatgg tatattgtgg tatatatcca ggagaaggag aaaagtatga gaatgttaga
     7621 gatgctcttg aaaagcttca agtaaatgat gctgcacttg aatatgaagc tgagacttca
     7681 gcagcactag gatttggttt tagatgtggt ttcttaggtc ttttacatat ggagataatg
     7741 caagaaagac ttgagagaga atttaacctt gatataatta ctacagctcc atctgttata
     7801 tatagagtta caaaaatgga tggagaggtt gtaatgatac aaaatcctgc aaacttacca
     7861 gaaccatctg agataaagat gatagaagag cctatagtaa aaggagatat aatagttcct
     7921 aaagactatg tgggtgttgt tatggagctt tgccaggaaa gacgtggaaa tatgttaaat
     7981 atggaatata tagatgagag aagagtaatg cttcactatg accttcctct caatgaagtt
     8041 gtttacgatt tctttgatgc tttaaaatcg agaactagag gttatggttc tcttgattat
     8101 gaggttaaag gttatgtagc atctacactt gtaaaacttg atatattaat caataaagag
     8161 caagtggatg cacttagttt tattgtccat gagacacgtg ctttccctag aggaaaggca
     8221 atgtgtgaga aattaaaagg agaaattcca agacatcagt ttgctatacc aatacaagct
     8281 gctgttggta ataaggttat tgcaagagaa acaataagtg ctctaagaaa agatgttctt
     8341 gctaaatgtt atggtggaga tatatctcgt aagaagaaac ttcttgaaaa acaaaaagaa
     8401 ggtaagaagc gtatgagaca aattggttct gttgaagttc ctcaaaaagc atttatgtct
     8461 gtgcttaaat tagatgagta aatccttata aatttagata aagatattta aaagctagag
     8521 tgattttaga attatttcta aattagtcta gcttttttat ggtatatttt aagtaaactc
     8581 atagagaata taagtaagaa tattaaagaa gaataaaaag gtgatattta tgattaatat
     8641 agaaattcct aaaaaagtag actatataat taaagaatta gaaaagaatg gttatgaagc
     8701 ttatatagtt ggaggatgtg ttagagattg tttattagaa agagttccaa acgattggga
     8761 tataacaact agtgctagac cagaagtggt agtagaatta tttgaaaaaa caattccaac
     8821 tggtatacaa catgggactg taacagttat gattgaacat gaaccatttg aagtgacaac
     8881 ttatagaatt gatggaaatt atagtgatgg tagacatcca gattcaatag aatttacaaa
     8941 taacatagtt aaggacttgt caagaagaga ttttactatc aattcaattg catacaattc
     9001 aaagacagga ttagtcgacc catttaatgg ctatgaagat atacaaaata agtatataca
     9061 ttgtgttgga aatccagtag atagatttga agaagatgca cttagaatgc ttagagcagt
     9121 tagattttca gcacaactca attttaaaat agctgaaggt actaagcaaa gtattcataa
     9181 aaaagcagat ttgataaaaa atgtttctat tgaaagaatc cagacagagt ttaataaaat
     9241 attagtatct gattcatcta aattaaactt attaaaatca acaggtttgt tgaaatttat
     9301 aattcctgaa atatgtgaat tagaagatgt tactcaacac aatccatatc acatctatga
     9361 tgtacaaaaa catactttaa tagctacaga agcaattgaa gatgaactgt atttaaaact
     9421 tacaatgttg tttcatgatt taggtaaaaa agtaactaaa acaactgata aaaatggtgt
     9481 tgaccatttt tatactcata gtagagaatc tgtaaagatt gcaaagaaga ttttgaaaag
     9541 acttaagtat gataattata ctattaataa agttttaata ttaatacaat atcatgatta
     9601 tagaatagaa cctaaaagga agataattaa aaagttatta aataaacttg aagatgttgg
     9661 attatttgaa gatttaataa aagttaattg ggctgataca cttgcaaaaa atccaaagta
     9721 tgcaaaacaa aagatattaa atcttattga gtgtgaaaaa gaatttaaac atataataaa
     9781 tcaaaaggag tgctttaatc ttaaagattt agctattaat ggaaaagatt tgatatctat
     9841 aggtataaaa ccaggtaaag atataggata tattttaaat aagatgcttg aaatagtcat
     9901 aaataatcca gagttaaatg aaaaagaaat actaaaagaa aaagcattaa atatatatac
     9961 attttagatt caaatataaa aaatttaatt actttaaaaa acatagaata aaatacttat
    10021 ttagataaaa taaataattt ttagagaata ttttagaagt tacgattgta tgatttttaa
    10081 atgcataggc atttactcta ggactataga ctaatataaa aaaaaacttt gggaatacta
    10141 aaaccataaa tttataaaat taatatttta tatactgtaa aacagtgagg agggacagta
    10201 tgcatactca actacaaaag actattggtc tttcagcagc tctttctact gttgtaggta
    10261 tggttatagg ttcgggagtt ttttttaagc cacaagcaat atacactaca actaatggag
    10321 ctccaggtct aggtattata gcgtggcttt taggtggttt tataacaata actgctgggc
    10381 ttacagctac agaaatttca gctgctatac caaaaactgg tggtatgatg atttatatcg
    10441 aagaaatata tggagaaaag ttaggttttt taactggatg gatgcaaact gtattatttt
    10501 tcccaggtac atcagcggca ttaggagtaa tatttgctca acaagcatct gaacttttag
    10561 gaatgagccc aaataatatg gcaaatgtac ttcctatagc aattggagtt atactatttt
    10621 tagcgttatt aaatataata ggttcatcct taggaggaaa agttcagaca gtagccacta
    10681 ttggaaagat gataccttta attttaataa tagtctttgg atttattaaa ggtcaaagta
    10741 gtgaagtatt aaatccattt gtaggagatg gtgtaaatgc atcaaatgca cttggacaag
    10801 tattaatagc tactttgttt gcatatgatg gttggataaa tgttggagct atatcaggag
    10861 aaatgaagtc tccagagaaa gatttaccaa gagcaatagt tggtggttta tcattagtta
    10921 tggcagttta tattataata aatatagctt atctttgggt agtaccagca agtgaattag
    10981 caacagttac ttccccagct acattagtag caacaagatt gtttggaaat ataggtggaa
    11041 aagtaataac tgtgggtata ttaatatcag tatttgggac attgaatgga tatttattaa
    11101 caggttcaag gataccttat acattagctg aaatgggaac tttaccagca tctaaaactc
    11161 ttttaaaagt aaattctgga ggttctcctg taaattcaat actacttata actgtgttag
    11221 cctgtgtata tgcattatct ggtcaattta atcttttgac agacttaaca atattttcaa
    11281 tatgggtatt ttatgtattg acttttatag gtgttatgag gcttagaaga caaaagccag
    11341 atttacacag accatataaa gtccctttat atccaataat accaattata gctatattag
    11401 gtggactgtt tgtaataata aatcaaatat tgacttcaac agtcatatct ttaggcggaa
    11461 tatttataac actattaggt ttaccagttt attattatat gaaaaaaaga taactactta
    11521 atttaagtta gataaatttg ggtaaaggtg aaaattaact tttatccaaa ttttttatac
    11581 tgttatacaa tataagtatt atatattgct tgatgtgcta ttttgtttga tttataattt
    11641 agtaattgat gttttaagat aatttaagaa atgaggagtg aaagatgcta ggactttatg
    11701 tacatattcc attttgtgtg aaaaaatgta agtattgtga ttttaattct tataaaatgg
    11761 acatagattc aaaaaaaaga tatatagaag acttaaaaat agaaatggaa ttatatagta
    11821 ataaattata taaagataat aaatataaaa ataaagaatg ctgtagtcta aataaaaatg
    11881 acaaaataac gagtatattt gtagggggag gtacacctag tatactaacc tctgatgaaa
    11941 taagagaagt atttataagt ataaaagaaa tgtttgatat agatgagaat gcagaaataa
    12001 ctatagagtg taatcctggg acattaacat tagaaaaatt aaaaactatg aaagagattg
    12061 gcataaatag acttagtata gggctacaag ctattcaaga aaagcatttg aactttatag
    12121 gaagaataca tacttatgaa gaatttgaga aaaactataa agatgcatta agtgtaggtt
    12181 ttaaaaatat taatgtagat ttaatgtaca gtcttccaaa tcaaactcta tgtgattgga
    12241 aggaaactct tgaaaaagtt gtacacttaa atccaacaca tatatcagct tattccctta
    12301 tactagaaga aggaactgaa ctatacaata tgtatgaaag taataaattt gaacttatag
    12361 atgaaaatgt agatatagaa atgtatgagt atacaataaa ctacttaaag tcaaaaggat
    12421 ataatcaata tgaaatatca aactactcaa aagaaggcta taattgtgag cataatattt
    12481 tatattggga atgtgaacat tatataggga ttggagcagg agcttctggc tatattaatg
    12541 agaacagata caataatgta gaatctttag aagactatca tttaagtttg gtaaaaagag
    12601 aaaaaccaat acaagaaaat gaaattcttt ctgagaaaga tatgatagaa gaaaagatat
    12661 ttatggggct tagaatgaat aaaggcataa aatttgaaga ttttaaaaaa aaatttggaa
    12721 tagattttag agagaagtat aataagcaaa tagaaatgct attagctaga aagcttataa
    12781 atcagagctt tgaaggtata caattaactc aaaaaggaag agaaatatct aatagtgtat
    12841 ttatagaatt tatggaataa tttttaaggt gttgacaaaa taaatttata atggtatata
    12901 atatatataa tctttttagc actcgaactt agtgagtgct aacagctagg aggtataata
    12961 atatggaatt aaatgaaaga aaattaaata tcctcaaagc tatagttaaa gattatatag
    13021 aaacagctga agctataggt tctagaacaa tatctaaaag acatgattta ggtgttagtg
    13081 ctgctaccat aagaaatgaa atggctgacc ttgaagaatt agggtattta attcagcctc
    13141 atacttctgc gggtagagtt ccatctgaaa aaggctataa gttatatgta aactctctta
    13201 tgagtaaaag cgaattggat gataatgata agattcttat tgaacaatgt atgaatcaca
    13261 atataaatca tataaaagaa ttgattcatg aaacttctaa gctattatcg caactaacaa
    13321 actatacaac tgtagcagtt actaagagtt taataaatca aagtgttata aagcacatac
    13381 agttggttgc tatgaatgac aataatattt tacttatagt tgtaacagat aaaggggact
    13441 taaaaaaagc taaccttaca actaatgtat atttagatca atctaagtta aatttgattt
    13501 ctgataatct tacaaggaag ctcttaggta aaagtataac agatttggat gataatctaa
    13561 ttgcttttat taaatacgaa ataagtgagt attctgggct tatagatgag cttttaaatg
    13621 ctttgaactc aaatatgaaa gaagaagatt tttctctatc attaaatgga gctactaaca
    13681 tatttagtta tccagaattt aatgatgtat taaaagcaaa gtcattttta aatatgttgg
    13741 aaaaaaagga aacaatagct gacataatta aatcaaaggg aatacaaaaa gacaatctaa
    13801 atataataat tggtagtgat aatgattgtg aactggcaca ggattgtagt atagttacag
    13861 caacttataa tgtagataga gacttggttg ggcgaataag ctttataggc cctaccagaa
    13921 tggattatgc tagaatttac tctataataa attatatgag tttattaatt aatagaaagt
    13981 aaagaaattt taaattgcga ggtgtaaagt cttggataaa aaaaatgagc aacaggaagt
    14041 aagagaagaa aatgatactt ctattaacca agaatcggaa actcaagtag agttagaaga
    14101 ggaagttgtt aatgaagaat gtgaaacatc aagtgaaaaa actgatgaaa aagaagttga
    14161 tgatgaaaat gtaacagata taaattctaa gttagcagaa aaaaaattgc aggatgagtt
    14221 ggatgaatta aatgataaat atcaaagact tcaagccgaa tatgcaaact acagaagaag
    14281 aactcaacaa gagaaagaga caataggcgt atttgcaaat gaaaaaataa taactgaatt
    14341 gataccagtt atagatagta tggaaagagc cttagatgca tgtgaagata aggaagatac
    14401 tatgtataag ggtataagcc ttgtacataa acaattaata gacactttag taaaatttgg
    14461 agtagaagaa atagaagcag aatctaagga atttgaccct aatctacatc tagcagttat
    14521 gcaagaaagt gtagatggag ttgaagcaaa tcaaatagta atggtacttc aaaaaggata
    14581 taagctagga actaaagttg taagaccttc aatggttaaa gtttcttgct aagtataaat
    14641 taaaggttaa attatttaac ctaatataaa atattaaaat aagtaaaact taattatata
    14701 attactcgag gaggatttta gtcatgggaa aaataatagg aatagattta ggaacaacaa
    14761 attcatgtgt tgcagtgtta gaaggtggag aagcacaaat aatagcaaat agtgaaggta
    14821 tgagaactac tccatcagta gtagcattta caaaagatgg tgaaagaata gttggagaac
    14881 ctgcaaaaag acaagcagtt acaaatgcag ataaaacaat aacttctata aaaactcata
    14941 tgggaactga ttataaagta aatatagatg gaaaatcata tactccacaa gaaatatcag
    15001 caataatatt acaaaaatta aaatcagatg cagaaagtta cttaggacaa acagtaacag
    15061 aagcagttat aacagtacca gcttacttta cagatgctca aagacaagca actaaagatg
    15121 ctggtagaat agcagggctt gatgttaaga gaataataaa tgagccaaca gcagcagctc
    15181 ttgcttatgg tatggataaa ttagaccaag aaaagaaaat attagtattt gacttaggtg
    15241 gaggaacttt tgacgtatct atacttgaaa taggagatgg aacttttgaa gttttagcta
    15301 cagctggtaa caatagatta ggtggagatg actttgacca aatagtaata gattatttag
    15361 cagaggaatt taaaaaagct gaaggtgtag atttaagaaa tgataaaatg gctcttcaaa
    15421 gattaaaaga agcagcagaa aaggctaaga aagaattatc atcaacaatg agttcaaaca
    15481 taaacttacc tttcataact gctacagcag aaggaccaaa acacttaaat atagatttat
    15541 caagagctaa atttgaagaa ttaactagag gtttagttga aaaaactatg gaaccaacta
    15601 agacagcttt acaagatgca ggactttcta caggtgatat agatgatgta ttattagtag
    15661 gtggttctac aagaatacca gcagtacaag aagctgttaa gaagtttata ggaaaagaac
    15721 ctcataaagg tataaaccca gatgagtgtg ttgcagcagg tgcttctata caagctggag
    15781 ttttagcagg ggatgttaaa gacttattat tactagatgt tactccatta tctcttggta
    15841 tagaaactat gggtaatgta atgactaaga taatagaaag aaatactaca ataccaacta
    15901 agaagtctca aatattctca actgctgctg ataaccaaac agctgttgat atacatgttc
    15961 ttcaaggtga aagaagtatg gcttatgaca atacaacttt aggaagattc caattaacag
    16021 atataccacc agcacaaaga ggaatacctc aaatagaagt tacttttgat atagatgcaa
    16081 atggtatagt taatgtttca gctaaagatt taggtacagg aaaagagcaa aaaataacta
    16141 taacatcaaa cactaattta tcagaagcag aaatagagca aaagataaaa gaagcagaaa
    16201 tgaatgcaga agctgacaaa caaaagaaag aaaaaataga agcattcaat caagctgaat
    16261 ctactatata tcaaactgaa aaaactttaa atgaacttgg agataaaata agttcaggtg
    16321 aaaaagagga tatagaaaaa gctatagcag atttaaaagc tgtaaaagat aatcaagatg
    16381 ctacagcaga agaacttaag aaagctacag atgaagtaat gactaagttc caaaaagtat
    16441 ctcaagaaat gtaccaaaaa gcagctcaag aacaacaagc ggcacaaggt gcagaacaag
    16501 cacaagacaa tggacctaaa gatgacaatg tagtagatgc tgactttaaa gaagtagatg
    16561 aagataaata agctatataa tatgtaacac taaatgataa ttggaacata tatgatttgt
    16621 gaaaatcata tagatgttaa agatatatgg acaatataca tcataaagaa tataatatat
    16681 catagattat ataacttaaa gtagaatata agaattaatg atagcttgta gtttattatt
    16741 ctacaagcta tcgttttgta aaatgaaggt ggtgtcaatt tttgagtaca aaaagggatt
    16801 attatgaagt cttaggtatc agtaaaggtg cagaagctca agagataaaa aaagcttata
    16861 gaaaattagc aatgaagtac catccagata gaaatcctgg agataaagag gcggaagaaa
    16921 aatttaaaga gataaatgaa gcttatgaag ttctatctga tgatacaaag agaaaaactt
    16981 atgaccagtt tggacatgat gggctaaatg gtcaaggcgg ttttggtggt caaggaggat
    17041 ttggcggtca aggctttggt ggttttgaag atatgttcgg agatatattt ggtgacatgt
    17101 ttggaggaag cttcggaggt ggaagagcaa gaagaagagg acctcaacgt ggtgcagata
    17161 taagacagag tgttactata tcttttgaag aagctgcatt tggtaaaaag atgtctataa
    17221 aagtaaatag aagtgaagag tgtgaagaat gtaatggaac tggagcaaaa ccaggaacat
    17281 caaagaaaac ttgttcaact tgtaatggaa ctggtcaagt tagaactgtt caaagaactc
    17341 catttggtaa tatagcaagt tcaagaccat gtagtgcttg taatggaact ggagaagtga
    17401 ttgaatctcc atgtagtaaa tgtcatggaa ctggaaatac aagaaaagtt aagactatag
    17461 aagtagatat acctgctggt atagatgatg gtcaaatgat aaaactttct ggtcaaggtg
    17521 aagtaggaga aaaaggtgct ccaagaggag acctatatat tgtagttaat gttaagtcac
    17581 atccactatt tactagagat ggaaatgata tttattttga aatgccaatt acatttgttc
    17641 aagcaacttt aggtgatgag atagaagtac ctactttgga tggaaaagtt aaatatagtg
    17701 tgccagaggg gacacaaact ggaactgtat ttagacttaa agagaaagga atccctagaa
    17761 ttagaggtaa ttcaagagga gaccaatatg ttaaggttgt agttgaaatt cctaaaaaat
    17821 taaacgataa gcaaaaagaa ctattgagag aatttgcgaa ggagtgtggg tcgaacgtcc
    17881 acgaaaagaa gaaaaccttc ggacaaaaga tagaagatat gtttaagaaa aagtagttaa
    17941 agtagccaat atataatcat atttttaaaa tcttagttat ttataagatt gtcaaaggag
    18001 atattatgat ttttatattg gctatttttg tataaaaaat aaatgtgcat aaaataaaac
    18061 tgaatataaa taattttctg aaaaatactt gtcctaaaat atataatgtg ctataatata
    18121 atcaaataaa agaaaggggt gattccagtg ggaacggatg tggcactgcc aaatgatgaa
    18181 gaaaacttta ttgtaatata caaaataaaa ttagatactt ttagtgctag attaagtact
    18241 ggaaaaactc atgagctggg ataattattg taattaccta gactcaaact tttagtagta
    18301 aaatagtaat attatataga caaggtatct aatttttaaa tcttttgtat gaattaaaat
    18361 ttttataaag ggtatatctg attagaatgt aattagggaa acctagttgt gaattacttt
    18421 tagtatatac atgtgtaaag aaatatgtat atggtattgg ttattgtgct agatgtgtat
    18481 gtaaagaaat atgtgtctag ttgggtatat gtaaagaaat atacttataa tctatgaaaa
    18541 atttttagta taaattgcta aaacattgag attgtttttt atagaaagtt agtttatacc
    18601 aactaaaaat gtgcttaaga tttgtttgat gtttaattat aaataaggtt gtagataaaa
    18661 aaataagtaa agacaaaggt ttaaaatact tgctttaaat atagtttgtt ttgaacactt
    18721 gccaaattaa aataattaat aaaatagaca tcttctcata gtaaaatata ctgtgagagg
    18781 atgtttttgc tttgttcata aacttttatg aaaaatttgc atagtgtcac attttaatat
    18841 actatatata taataaaata tagataaaat taaatgattc aaaagagaaa ggtgaaaaga
    18901 gatgtattat ataggtgtag acataggagg aacaggtata caagcaggag tagttgataa
    18961 ctatgggaaa ataattttta ggtcagagtg taaaacagta attgaaaaag gatttgaagg
    19021 aatacttaat gacataaaaa taatgattta taagttgttg gaagataata aacttactat
    19081 gagtgacata aaatctattg gatttggtgt tccaggtttt ataaataaag aaggattagt
    19141 aacttgtgta aatttaaaat ggaataaaaa agcttttaat aaagagttaa aaagaagatt
    19201 tccagatgtt gaaattcatg gagaaaatga tgcaacagta gcagcacttg gagaagcaaa
    19261 gtttggaagt atgaaaggtg ctaatgttgg agtattatat actttaggaa ctggtgtagg
    19321 tggaggaata gtaattaatc aaaaagtctt ttctggagca catggattgg gttcagaaat
    19381 aggtcatcaa attataggag aaaactattt taattgtaat tgtggaaata atggttgtgt
    19441 agaaactttt tgttcagcaa ctgcaattat aaagtattct caaaaactga tagaagaagg
    19501 agaaaagagt aggattttag atttggcaga gggaaattta gaaaatgtaa atgcaaagat
    19561 ggtgtttgat gcatatagag aaaatgattt agtggcaatt aaagtaataa atagatttaa
    19621 agagtattta gcaaaaactt ttgcaaacac aataaattct ttagacccag aaattatatc
    19681 tataggagga ggcatttcta aatctagcga tataatactt gatggaatag aagatttggt
    19741 aagaaaattt gtattataca aaacagaaga tatagctaca attacatgtg caactttagg
    19801 ttcagacgca ggaattatag gagcagcttt tttataatgt aaatgaactg cttattggag
    19861 gcgtgatgat atatgggtaa aacaaatgct agaagaacgc tttttttgat agcaataggt
    19921 tcaggaacta tgctgaatcc tcttaattct tctatgattt cacttgcttt acatagtata
    19981 caaaatgatt ttcatattag ttttacaact gtatcttgga ttgtttcagc attttaccta
    20041 gctaatgcta ttgcacaacc agtttcaggt aagataggag atttaatagg tagaaaggtc
    20101 ttatttttga gtggtttgat gcttgtaatt ttatcagcat ccatagtacc acttgttcaa
    20161 tcattcttta tattgatttt tataagagta attcaagcaa ttggaacaag taccctatat
    20221 ccatctggag ttgctcttat acaaaataat ataaaagaaa gacaatcttc tgcattagca
    20281 gttctaacca tttttgcttc aactacagca ggattaggtc cgacacttgg aggattattg
    20341 ttggacttag gaggatggca tgctatattt cttgtaaata tacctgttgt tttggtttct
    20401 atatgcctag ggtatttttt attcccaaaa gaagtaaaag aaaagaaatc tataaaagaa
    20461 actttaaaaa atgttttgtc aagattagat attattggca tacttctatt ttcaattgca
    20521 atgattttta tattactatt tttgctatct atgaaggaat cttttaactt agaacaactt
    20581 gtatttggta taatactgat gtgtcttttt atttggcatg aattgagaac aaaattgcca
    20641 tttatagata ttaagctttt tgtaagtaat cctaaattgt caaaagttta cttgcaattt
    20701 attatattaa atttctttaa ttatattttg ttttttggtc ttcctagtta ttttcaagat
    20761 gccttacatt atagtgcaaa gtcgactgga ttatttatgc tatttatgtc tggtataggt
    20821 atatttatat ctccattaac tggaaaatgg atagataaat ctggaactag atttcctctt
    20881 gttacatcat caatctttat gtttattagt gcagtacttc tttcagtagt ttttatccat
    20941 atacctgtaa taggaaaggg tattatatta tcattagcag gtattagtta tggagttggt
    21001 aatgtagccc tgcaatcctc tatgtttgaa gaaagtcctc gtgacagtat agggacagct
    21061 tctggtctgt ttcaaactag tcgttattta ggttctattt tagcaactgt tttacttgga
    21121 atggtatttg aagtaaatat aacatcagaa caatttcaga tattaggtta tgttatggtg
    21181 gtcttaggta caataagttt tttattaaat tttatattaa agaaagaatt aagaaaagta
    21241 gaatagtgga tttatggatt taataagtct aataactgat aagtttaaaa atattgtttg
    21301 taaaacataa ctggtttgat atttattaat tctaagtttt gaagcataga gtactcgata
    21361 agtatacaat attttgaatt aaaattaagc agaaatttga actagtcttt caaatctctg
    21421 cttaatttag tggttaaatt tttttataga gaaaataaga tttgacactt agataaaaaa
    21481 tcattgcatt ttcaaaattc tttaaatcaa acccaagtat ctcattaatc ttttccaatc
    21541 tatatactag cgtgtttcta tgtaaaaaca tactagtaga tgtcttagaa atatttaaat
    21601 cattatttat aaattttatt cctgtatata gaagttcttc atcaatagat ttaatcttat
    21661 taaaatctat taatttattg agtatattat cctcattaat taaatctaaa gttttatatc
    21721 taataaggtc aaacaaattt aaaaacttat tttcagcgtt gtcttttata atatgaggta
    21781 gtaaatcaga acataactgt aatctaaaat caaaatcagc tttactatct aatttaccaa
    21841 gataaaaaat ataagtactt tgataagttt cagtttcaat tatattttgt gtagttacat
    21901 taatatctaa gttatattta gaatttacta taaaaaaacc attgtgttgt attatattta
    21961 tatcttcaaa taaattataa aaaatatttt ttagatattc aaagtctata tgagattggt
    22021 aaattaaata aaagttgcct tttaaattat caagattatt tttatctaga aaatcaataa
    22081 cataaatagc attattgaaa gtaactaaat ggctactagc atctaacttt tggttttcat
    22141 atggatagat atttattttt ttattaagct gtctttctaa aaaatctttt aactcttcca
    22201 taaagtctcc ttaaaaataa gattatattc ttatcttata gctaaagtag tttctttgtc
    22261 aaatatgtgt actctactat tgtcaactga aagtgttaag tcatcaccaa cattaattct
    22321 gtttttactg tcaaatctaa ttgttatcgt attttctcct agttttaagt gagcataagt
    22381 ttctgctccc ataagttctt ttatttcaat tcttgattta aaacaagtat ctggattaga
    22441 tttaataaaa gtatcttcta catgaatatc ttctggtctg atacctataa caacttcatt
    22501 attaatataa ttgtctttta ttaaatcata gtgtttttct ttattcaatt taagcttaat
    22561 atgttcattt tgtgcataaa tttcatcatt ttcttctatt aatattacat caataaaatt
    22621 catctgtggt gctccaataa atcctgcaac aaatatatta gctggataat cgtatacatc
    22681 ttgtggagtt gcaatctgct gaactactcc atccttcatt actacgattc tatcagccat
    22741 agtcatagct tcaacttggt cgtgtgttac atagataaat gtagttccaa gctgttggtg
    22801 tagcttagtt atctctgttc tcatagcagt tctaagtttt gcatctaagt tggatagagg
    22861 ttcatccatt aagaatacct taggatttct aactattgca cgtcctaagg caaccctttg
    22921 tctttgtcct ccagataatg ttttaggttt tttatttaaa agaggtagta agtctaatat
    22981 tttagcagct tctttaactt ttttgtctat ttcatcttta ggcaattttc taagtttaag
    23041 tgcaaatgcc atgttttcgt aaacagataa gtggggatat aaagcatagt tttgaaaaac
    23101 catagctata tctctatctt ttggttctat atcattgaca agtttatctc caatgtatag
    23161 ttctccttca gagatttctt caagaccagc aatcattcta agtgtagtcg atttaccaca
    23221 accagatggt cctactaaaa cgataaattc tttatcttta atgtctatat ttatattttt
    23281 tactgcatta aatccatttg agtaaagttt tgatatattt tttaaaatta cttctgacat
    23341 aatatcctcc ttgatattta aattttaatt atatagtatc aaattttttt attatattaa
    23401 atgtgtaaaa tgactaaatg tatttgttta atatgtatat ttagttttta gcaaagaata
    23461 aaactaacag taaaatatat ttaaaaaatt aaaaaacaaa attaaatgta ttatcaaaaa
    23521 atcattttat gatatgtttt atttttatat tacaaataga gaataatgaa ttatactaga
    23581 aagtaggagg tgaaaatatg agcagaattt caatttttat ggaatcatgt aatggcaaat
    23641 ttccaaatga aaattcaatg ttgtcggttt cctttataaa aaatatttta aatgcagaaa
    23701 ataaagattt tgctaaaaat atatttaact atggggaaga aagaaaaaac aataagcaaa
    23761 taaaggatat aaatacagcc atttacatac caaatcttat aagaagcaaa aatgagttac
    23821 ttgttgatgg agatataata ttcaatataa gtttttataa ttattctatg ttcagtaaat
    23881 tatacaatgg aattttaaaa gtaaaggagc tagaatataa aggttataga tttaaaataa
    23941 aaaatataaa aatacataaa gaacacgaga taaaagaaaa tggtgttata tttaaaacta
    24001 tgtctcctat aatagtaaag aataaagaag gtaaatattt agatgtagaa gattcaaatt
    24061 atatagaatg tttaaactat atagctaatc taactctgga gagtgtaaga ggtagtgggc
    24121 ttagaaagcc attggaattt atacctctta acttcaaaaa aagagtgtta aaagaaaaga
    24181 ttcgtggatt taaagtgaga gaattttact atataaatgc ttatgcagga acattttttt
    24241 taaaaggaga tatggaagat ttaaatgcac tgtataaaat gggaattgga tatagaagga
    24301 cggaaaatgc aggaatggtt gatatattaa agtagcgagg tgagagaaat aaaagctaaa
    24361 ttttatagat caggtgatat tgtaagagat attggcattg tttactttta tgaattacta
    24421 atggatttaa aaaacgaact agaaaaaaat gattataagc taaaatttac ttgtgagcta
    24481 aatagaaatt atttatcttt agaatctgca gaatatatag accctaagta tatatctgat
    24541 tatatattag aaaatcaact ttttaaggta tttaaaaatg ggaaaaagga aaccttagaa
    24601 aaaatattta gtaatataga taatttaacg tatgtaaatt ttattgaaga attggataaa
    24661 tcttctgcaa ctgctaaaca aaaggaagat attaaaaaag attttaaaaa caggaggttt
    24721 ccatatgtta gaaatagtgg taagtatgga ctaaacacga gtttagaaaa tatggaaaca
    24781 aatgtaaaaa gaatagtaga tactgtaatt aaatcaaata tagagataga tgaaatagat
    24841 aaacttgatt tgacacaata tgaagaaaaa gatagtgtat gtaatgtttg taatataaat
    24901 aaaacaacaa aactagatat tgatttaaga gagcgaaagg actctaagta taacttttta
    24961 tttagaggtg cagaaaaatc aggctttaaa agaagtggac aagttgaaag taatatttgt
    25021 tttgagtgtg aatttttcaa cttaatgtgt cttttatata taaatttaaa aagaccaatg
    25081 gtttttgcat atacgaatga tttaagagaa ttagcatttt taaatcataa gataatgctt
    25141 aaaagaaaaa tgtattcaga taaatcattt tataaaaaac ttttgcatga aaaaataagt
    25201 tcaattagac tatatagatt tgatattgtt aagaataaag gtataattct aaagtttgac
    25261 tctattatag agtataaaga attattaaaa aaaattgaac ttatagatat tatagataat
    25321 tataatttct caagggagcc agtaaacact aaaaatttag gtaaaaaaat gattgataat
    25381 gggaatttat caaatttaaa agaattatta cttgataata taagtttttt aaagcaatca
    25441 actggaactt ctcgagaaat ggattttgta tctagctcat ataatatcgg tttatatata
    25501 aaattgtgtt ggattgtaga tggaggggag gagtataaaa tgaaaaacta tatggaaagt
    25561 aatttaatat attcaagagt aggtaaagac ttatttaata aaatgacaga tgaatccaga
    25621 agaaaaaatt tttctatgaa agtgattcaa ctacttaagt ctaatgatag aactcagcta
    25681 tttcaaacta taatgcatgt gttagtttct aatggaatct taataggaga agggtttgta
    25741 gaaggtctaa tgcagagttc tgaattagaa ttaaatacga atgtaggtct atttattcaa
    25801 gaaatcatga aataaaaaag ggggaaaatt taaaatgaat gaaaaaagag taaaaggagt
    25861 aacattaact atactgacag aaagcccagt tccactttca tatgaccaag ggtatggaaa
    25921 ttatacacca ataaaaaaag aacaatatag agaaaaaata catgcaaaaa ctagtatagc
    25981 cacaataaca tatgatttaa gaagaatgct ccaccaagaa tatggttgga atttaagtaa
    26041 tattgttttt ggaaaaaaag gaaatatata tccatctatt aagaaaaatg taggatgtgt
    26101 agatgaaaat ggattagaga ctgatgtatt tggttatttg ataccactta aagataaagg
    26161 ttctataagt aaagcttctc cacttagaat aatacctttt aggtcattaa atccatataa
    26221 aggttcaaca cagctaataa caaatagagg ttttttaagt agtgaatttg gtagaaaata
    26281 ttatgatgag aaggaagaaa atgaagtacc tagagatgaa aactttccaa catcacaagc
    26341 attagcaata gaagagactt tatcagatta ctacgtttat acaataacat tggagcttga
    26401 tagaatagga gtagtagaag tagaagatgg aaagttactt ttaccagaag aaagaaaatt
    26461 tatgagtaag gagttaagag aaaaagcagt aaaagatata ttagatgcaa ttactgtgtt
    26521 tacaagaaac ataaaacatt catcagtact attaaaacca cttgcagtaa tgggtggagc
    26581 ttttgataaa gttgtaccgt ttttttggga tgatgtagat tataatgctg atagtggtga
    26641 gataaattta gagggagtta tagaaactat agaaagttat agtcttaaag aaagtaatac
    26701 aatacttgct atcaatgaca gattaaaaat ttctaataaa aaggaattaa ataagtttaa
    26761 tttaagtaaa taccctgtaa aagagattaa aaatctagca gatagattgg aaattggaga
    26821 agataatatg tggtatttaa aagagtagga ggttttttga attatgaaag tattacaatg
    26881 taaacttaag caaccaactg ctcactatag agacccaaaa gtatttcaaa atgaatatat
    26941 aagtacttta aatttaccat ctaagaccac aataatggga atgataacgt atttatgtga
    27001 tagaagatta aactctgata tagatatagg aataattgga acacatcatc atagagaact
    27061 agaattttca agaggagaga acattgattt ttggaatgaa tatacaaata tgagaaaagg
    27121 caaagataaa gaaaagtttt tgcttcaagg aaactactat gattactata aagaacataa
    27181 agctcaaaat agcatattaa actatgaagt tcttaaagaa gtagaattga ctatatttat
    27241 ttcttgcaag gatgatgaag aacttgaatt tattagaaaa aagcttgaaa gtccatgtaa
    27301 atatgcaaat ttaggaagaa aagaagattt tgtaatacca agtgaaaaag ggtgttttgt
    27361 aaaagaagtt gcgttaaaag aggttatacc tatgaatact cgtgatgcta taaaagaaaa
    27421 tataaaatta aagaatacat atgtaagagt tgacttaaga gataaagaga atgtcgagac
    27481 tataataaat caaggtgtat tgattgcatt gcctcataaa tataaagatt tagaagcaaa
    27541 tagagatgat agagtttatg agttttgcca ttatatatat gttgataatg atggtatata
    27601 tcctaaaaat attaaggtaa atgtatgtat tgatacaaaa gaagttttta catggcttta
    27661 aattattttg aaagataggt gtttatcatg aaaaatatca tgcaaaaaga aatattagct
    27721 aagtcataca atttaagaac taatcaacaa gaacaaacat tagttgaaca tattaaggat
    27781 ttatttgaag tattagaatc tatattagaa ctaaatatat attcagataa agatgtagag
    27841 atactaaaaa tatgttgtgc tttgcacgat ttaggaaaaa ttaattcaat tatgcaacaa
    27901 aaaatggaga ttaataataa aatatcgtat agttgttcag aagaagagag gaaaaagtta
    27961 gagtctgaca aaaaatcttt aaaaaagata gcaagacaca atatattttc tggagcattt
    28021 ttaaaaaata tactagaaaa aatgaacttg tcagaagaag ataagttata tatatataaa
    28081 agtataatgt tacatcatgg aaactatgaa gattatatga ggttaagtac tagtaaagtt
    28141 caaaaagaaa tttatgatta tattgaaaaa ggtattttag aaaaagaaga ttttaatttg
    28201 gaagatatag aagattatat aaaggagatt ttatatgtag attttaaatt taatgaagat
    28261 gtattggatt atgattacat agataaatta agtgaaagta tggttatata cagtgattat
    28321 aacaatggtc aaatagaaga ttcagtttta aattatagaa agtttaagta tattttatat
    28381 aaagggatgc tgaacttaat agaccatagt gcatcaagta gacagaaagg tataaaattt
    28441 tataacgatt ttactgatga agaaattgat aatatgattt taaatgagat atataagagt
    28501 cagggtaatt tagaaaaaaa taatatagag ttcaatacta tacaaaaaag gttgagagaa
    28561 ctttctggaa gaaatgttct gactgttgca tttacaggtt caggaaaaac agctgcagat
    28621 tatagaaaga cttttaaaag aaaattcttt ttagtgccaa ataaaatatc agcagaaagt
    28681 ttttatagaa aaaatatttt tcaaaataaa aatgatttag atacaagaag tagtaatgat
    28741 tacattggat tacttcatgg agatataaac ttatattcag agaatgaaga taatggagaa
    28801 catgattttg tattgacatt aagggatata gatttgagta taaacttttg caaaccttgt
    28861 gtaattgcaa cagttgatca gttattactt agtatgttta aatttccggg atatgaaaaa
    28921 atattcgcag ccgtaaaaaa tgcatctata actgttgatg aagtacattt attggaacca
    28981 aagatgtttt tgattttgat atattttatg cagtttgcat gcaagtactt agatgttgat
    29041 tttcacttaa tgacagctac acttccaaaa tcatataaag agcaaatgat aaacaaggga
    29101 attatttttc aagaagaaag taatgaaaat gttactgata aaggagaaat agtttttatt
    29161 gagagcaaca aggaagaaga gatttgtgaa ggtaaagatg taaaggtttc ttttataaag
    29221 gaaaaagaaa tcaagtcaat agttgaagat gctttagaaa ataaacaaaa aattcttata
    29281 ataaagaata ctattgattc agtcaataga acatatgagt ttttaaaaga aaatttatct
    29341 gataagtatg gtggtgtaga tatagatgtt ttacatagta gatttaagtt caaagataaa
    29401 aaagagaaat atagtaaaat attaaatgga gagggtgata tttggatatc aactcaatca
    29461 gtagaaatat ccttggattt ggatttcaat ataataataa gtgatttagc aactatggat
    29521 agcttaatac aaagaatggg tagatgcaat agaaataata aatatgaata tggaaacttt
    29581 tatattctac ctagtgaaga taaaatttat gacaacaaac ttaagagcac aactaaaaac
    29641 atcttaaagg acatattaaa aactaactct atttttacaa tgggaattag aaaaactatt
    29701 ttggatgatt actatgataa tagtgtagtc aaaaaatatt ttgaagagaa ttttataagt
    29761 tgtgataaag aaattaagaa tatttatggt gtaaataaag aaatttttga tggattggat
    29821 ttaattttta attttgaacc ttacaaaaat attgttgata gtaagaagga agcagctaaa
    29881 atttttagag atgttgatgt tagttacaaa attattctgg aagaagattt ttataaggaa
    29941 gatagagagt tacaacaaga ttcgattcaa gtatcagggt ttatatttaa tagattatat
    30001 tattatgggt taataagcaa agtagaagga tacatggtac taaaatctag tagtaagttt
    30061 gagtataata gtactgtagg cttaattttg aagtgagcat atgtttaaat ataatatacc
    30121 tttagacatt gaaaatacaa aaagtactac tgtaagctca ctgtaaaaac tattttttta
    30181 aaaggaaaaa taagttgtga gatagagtgt ttacaagtgt atatagataa taaatttgag
    30241 gtttaagatt aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt
    30301 ttgtatgttt aagattaact aagtggattt caaatattga tatagtagat ataatagaag
    30361 ttttattatt aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa
    30421 ggatattatt agcttgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg
    30481 atataataaa agttatggaa aaacaggaat attactatag agaagagagg taaacttatg
    30541 aataattgga tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat
    30601 atactatatg aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc
    30661 caaaagaaaa atgaactaga ctgggattat gttgaagaag aagtatttaa aaagaatgaa
    30721 gaagatgatg ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc
    30781 gaaataataa agcaaaaagt tttaggatta aaagattttg gtatagatat aggtgaagga
    30841 agtgtatcat tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac
    30901 aaaccaacaa aagtgggaca aagggtagta gtaaaaccta catgggaaga ttatgctatg
    30961 caagaggggg atttaatcat agagcttgac cctggaatgg catttggtac agggacacat
    31021 gagactacta gtatgtgcat tagagaatta gaaaagtatg tgaataaaga ttctaaagta
    31081 tttgatattg gatgtggtag tggaatcctt gcaatagcag cagccaaatt aggagcaaaa
    31141 gaagttgtag ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt
    31201 gaaaataaag ttgaaaaaag tgtatctgta atgcatggta atttaaccga tgtaataaaa
    31261 gataaagcgg atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa
    31321 gatgttcaaa actttatgaa agaagatgct atattcatat cttcaggtat aattttggat
    31381 aaagtagaag aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa
    31441 aaattaggtg aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt
    31501 ataatggata gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact
    31561 atagaaggtg aagatgtaaa acatatttca aaagttttaa gatgcaaatt aggtgagaaa
    31621 cttgaaatat gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag
    31681 agtatagtta atttagagat tttagaaaaa gttgatataa atagagaatc agaacttaaa
    31741 gtaaggctat accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta
    31801 actgaagtgg gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt
    31861 gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca
    31921 aagcaaagca aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct
    31981 ttagaagata tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt
    32041 tcaataaaac atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata
    32101 ggtccagaag gtggattttc tgaagaagaa atagaacaaa tacaaaaaaa taattgtaat
    32161 gttgtatcat tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca
    32221 atagctttgt atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt
    32281 ttatacactt gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga
    32341 gaaagatggt tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac
    32401 atgtacagtt actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa
    32461 gaagaatcca gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccggaaga
    32521 aattttggat atagaagaag taaatttagt catgggtaca aatgatagaa gaaagattgt
    32581 tgaagaaatt aaaaagataa attcaagtaa gaaagttagt acagttgacg acataatgaa
    32641 agttaaagct tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat
    32701 gaaaatacaa gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg
    32761 acgcgttaga agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa
    32821 tggctataaa gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa
    32881 ggatagggat attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga
    32941 aagaattagg cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt
    33001 tttaaagatg gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga
    33061 aactctaaaa agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag
    33121 attgagaagt aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg
    33181 agagacaaat gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca
    33241 aatgcatatt tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca
    33301 agttgaccct caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa
    33361 ttttaataaa tttgcaacta aatttatcgg ccgtgagttg gatgttttgt ttgaacaaaa
    33421 tatagaaggc aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag
    33481 tgacaaaaat atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata
    33541 tgtagaagga attttgctat aacatgtaga aattataata tttataagat aggaggtgga
    33601 aagaatggat tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta
    33661 tgaagatgat agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt
    33721 agttgtacct aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat
    33781 tgtgtcacat attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac
    33841 tggattcaga gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca
    33901 ctatcatatt ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa
    33961 attattgaaa aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt
    34021 tataataaac aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc
    34081 ccagcataat tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga
    34141 gaatgaaaca ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat
    34201 catgtcagaa gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa
    34261 agctgaagca gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg
    34321 aaggggatgt cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa
    34381 gatacagtga gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt
    34441 gataataggg tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattaaag
    34501 caacgtagag atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa
    34561 acagaaggtg aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag
    34621 ttagaagaaa ttgtaaaatc tactatatct gaagtagggg caacttctat gaaggatatg
    34681 ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata
    34741 aataagttgg taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg
    34801 ctttttatag tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat
    34861 tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa
    34921 acatatttat accactaata ataggttact taagtagcat cttaacaatg attatcagtg
    34981 gaacagatat ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt
    35041 ttatctttcc tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa
    35101 tattaagaaa gggttacaac ttacctaaag taagagatgc aattttttat tatggattac
    35161 aacttgtatt aaactttatt tggagtatac tattttttgg atttggactt agatttacag
    35221 ctttgataga tataataata atgattttgg tttctataat aatgatttcg aagttttcta
    35281 aaatagataa gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag
    35341 gatttttgaa ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta
    35401 ccctgtactt ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca
    35461 ttttcatata caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga
    35521 ttaacatatt aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa
    35581 tagtatctgg gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat
    35641 taagtaggtt agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga
    35701 aaaaggatag acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag
    35761 cagaagatgt agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta
    35821 gacaagttgt caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa
    35881 gatttgaaaa tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg
    35941 atgaagaaga tgaaaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga
    36001 gttctgagga ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa
    36061 accaagataa ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa
    36121 tagaacaaga ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg
    36181 taatcatgtt gattttttat caatatgatt acctttttct tgtttaaata atatattaga
    36241 tattaggtat ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc
    36301 ataaatacct atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc
    36361 cactgattta caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat
    36421 agaaaattat ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt
    36481 taaaacaatt aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat
    36541 aggtataaaa gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat
    36601 aagttttata aggggatact atgttatagt tgtggaagga gtaagactcg aacaattttt
    36661 aaatcatctt ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa
    36721 aatggagttt catatagata gacaagatat aaaagaattt aagaatgttt atagaggaag
    36781 taagtttgat ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta
    36841 taagcacaaa ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc
    36901 acagtttgtg acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag
    36961 aaatgagctt tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa
    37021 agaagttaga gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt
    37081 aaaaggaaca aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga
    37141 ccaatcaaat tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc
    37201 tagaagtggt aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt
    37261 aaatggagct aataccaagt caataccaga agtatgggca agtacttttt atgagtccac
    37321 aaaaaaggaa agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata
    37381 tacactaagt ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta
    37441 tgttgttgaa aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat
    37501 aaaaacgagt actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt
    37561 aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc
    37621 tagaataata gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat
    37681 aacagttcaa actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga
    37741 aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc
    37801 agaaaaaaga ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa
    37861 agaccaaaac aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa
    37921 taatagcaat aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga
    37981 taatacaaaa gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact
    38041 ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag
    38101 gcaatataat tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg
    38161 aattacacca aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat
    38221 tatctctttt gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca
    38281 taactcaaag gggaaaagct gttcaaccta aaactttagg tcaaaaagaa tatataaaac
    38341 ttatagaaaa taatgatata acttttggtg ttggacctgc tggaacagga aagacttatc
    38401 ttgcggttgc tatggcagta aaagctttta aaagagatga agttagtaga attatactta
    38461 caagaccagc tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca
    38521 aagttgaccc atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata
    38581 aatttaataa gtatttagaa agaggtacta ttgaggttgc accacttgca tttatgagag
    38641 gtagaacact tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac
    38701 aaatgaaaat gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg
    38761 taactcaaac agatttacca caaaataaga agagtggtct aatacaagct acagagattc
    38821 ttaaaggtgt tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg
    38881 agttggtaca gagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa
    38941 aagaagaacg taaaaaagcg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta
    39001 gtaatataga tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat
    39061 actagatgat agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga
    39121 tataatcata gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc
    39181 ttttgtagat aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt
    39241 gactgatgtt ttatcatttc ctcttttaag tgatgatttt gaggatgttg agttagaaga
    39301 agaatcccta ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta
    39361 taaccatagt tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt
    39421 aggttatgac cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat
    39481 attaaataag ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa
    39541 ggtataatta aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa
    39601 agaaatatga aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt
    39661 aacttttcaa aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgct
    39721 gaaatgttta atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt
    39781 cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc tagttgcagc actaaattca
    39841 gtagtagttg gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt
    39901 tataaaataa gagaatcaga attacatata accttgatat gtatattatt ggttcttata
    39961 gctgttgtag ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca
    40021 agtggtcatg ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga
    40081 gttgttgcct ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa
    40141 ggaaaaatac ataccttttg ggagactatt gctggagctt tgttaggtgt gttgatagct
    40201 atattagtat ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa
    40261 aataaaacaa atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa
    40321 taacttttaa ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt
    40381 ggcagaagat gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc
    40441 tcttttaact aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg
    40501 tggaacaaat tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga
    40561 tatctataaa atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg
    40621 tggaatatgc aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata
    40681 tactaaagga gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg
    40741 aaatgattta aaataaattt aaaagtataa ataaatttaa aagtataaat aaatttaaaa
    40801 gtataaataa atttaagtaa ttaaaacaag aagataaaaa gttaggagac agttaatgtt
    40861 caaatcaggt tttgtcagta tagtgggtag accaaatgta ggaaaatcta ctttaatgaa
    40921 taatgtagtt ggagaaaaaa tagctataat gagtgataag cctcaaacta caagaaatac
    40981 aatacaagca gtatatacag atgaagaaat gcagatagta ttcttagata caccaggtat
    41041 tcacaaacca aaaaataaat taggtgaatt tatggtaaag gcagcaacag aagcttttaa
    41101 aaatgtagat ttaatattat ttgtagttga tgattctaaa aaaataggtc ctggagatag
    41161 aaagataata gaggatttaa gaagtgttaa gacaccaata attttagttg taaacaaaat
    41221 agaccaacta gaccaaaaag atgagctatt tgatataatc aaaatgtatg atagagaagg
    41281 tatttttaaa gaaatagtac caatatcagc attaaaagga aagaatacgg ataccttaat
    41341 aaaagttata caaaattatt tagaagaagg gccaaagtat tttccagatt atatgattac
    41401 agatcagcca gaaagagtat taatagcaga acttataaga gaaaaagttc ttcactatct
    41461 taatgatgaa atacctcatg gtgtagctgt tgaaattgaa aaaatgaaag cgagaaatga
    41521 taaggaaata gtagatgtat ctgctgtaat atactgtgaa aaagattcac ataagggaat
    41581 aataatagga aaaaatggta ggaaactaaa aggaataggt aagtcagcaa gacaagatat
    41641 agaattgctt ttaggttcac aaataaatct tcaattatgg gttaaagtca aagaaaattg
    41701 gagaaattta caaaattata taaataattt tggatataat gataaataaa ggtgggttat
    41761 atggatagga aactggatgc ttcccaagat ttattatatg aagtaaaatc attaattgat
    41821 aataataaag tattggagtt aagagaatta atagaggaat atcatataat tgatatattc
    41881 gatataatgg aaaatttaga ggaagacatg aaaattcaat tatttgaagt acttccttta
    41941 gatatggcat cttctatatt agaagaaggt agtgtagagt tttttattag tattttatct
    42001 aaactggatg tagaacactc gaaaaatata cttgaactta tgtctcttgg agatatggca
    42061 gacaagttga gtgaacttga agaagaagaa agagaacaca taataaatct tttgaatcaa
    42121 gagaatgccg attatgtaaa agagttacta ttctatgatg aagattctgc tggtggaaca
    42181 atgaccactg gatatatttc tataaataaa gatatgacag ctcttgaggc tatagaccat
    42241 atgagagaag aagctgaaga agctgaaact atttattata tatatgtggt tgatgatgaa
    42301 gaaaaactag ttggagtatt gtcattaaga gaattaataa tagctagaga cgcaaatata
    42361 gtagaagatt tgatgagtga aaatataatc tctgtttatg tagatgaaga tagagaagaa
    42421 gcagtaagat tagtatctaa atacaattta atagcaattc cagtagtaga cagacaagaa
    42481 aaattaaaag gaataataac agtagatgat ataattgatg taatggaaga agaagcaact
    42541 gaagatatgt ataaatttgc aggaagttca gaacatgaaa gagaagttgc tgaaaaagaa
    42601 aaccctacat tgagagagca aataatatca gcacttagag gacgattacc ttggttaatt
    42661 attaccttag ttggagggtt gttggcttcg ttaatacttt ctaacttaga ctatataatg
    42721 aatcctgtat atgctccatt agtatttttt atacctgtag taattggtat gggtggaaat
    42781 attggaactc aatcatcatc tgttacagta ataacattat ctaataagga tttaaatttt
    42841 agcaatgtgg ttagagaagg tattgtaggt attattacag gtcttttgtg tagtataata
    42901 acaggtattg taatttactt tgttatgaga aagttagaca ttgtattaat tgtttcaata
    42961 tcattattta taaatatggt tttaggtgca acaataggtg cgtttatgcc tgttttatta
    43021 aaaaaaatgg atgcagaccc atctacagtt tcatcaccaa ttatatctac agctcttgat
    43081 ataactggta tagcagttta ctttataata acaacggcat tattgtcaaa aattgtttaa
    43141 taactttatg tatgatttat cctcttctta ctaaaaatat aagtataaat gtaagaaggg
    43201 ggttttttaa aaatgaacaa tatcagttgg gaagttttta aaaaaacagg tagtatagat
    43261 gcatatttat atttctgtga ttgcaaaaac atacatgaag aaattgaaga agtaagggag
    43321 aaaaaagaag acgatgataa tcctgaacac ccaggggata gtacttaaag ctataaggta
    43381 taaggaaagt gacataatac ttacactatt tacaagaaag cttggaaaag tatctgcaat
    43441 agcaaaagga gctaaaaaaa ataagagctc tttgctttca tcatcacagt tattttctta
    43501 tagcaatttt accttaaaaa aacaaggtaa tatgtacaag gttactcaaa gtgaaataat
    43561 aaaaagtttt tataatatat cctatgatat agaggcattt tcttatgcta catatattac
    43621 aaaattagtt gaaaattcta tacttgaaaa tcagactaat aataggctat ttatattact
    43681 tgcgcagact ctgtatttgt atacacagga taatacagac aataggttta taacagcagc
    43741 attcgagtta aaatttttag actatatagg atttaaacct atagtaaata aatgcattaa
    43801 ttgtgaatgt aaaattttac aaaattctgt gtttaatatc tacgaaggtg gtatactatg
    43861 tagtaagtgt agtaaacttt ttgataataa tataaaatta gacttaacaa ctataagtct
    43921 tatggaatat gtattaagaa atgatatttt aacatgtagt aaagctaaag tatcaaaata
    43981 tataacacat gaacttgaga atattctaga caagtatttg aaagtttatg ttgataatat
    44041 taattttaaa tcattacacg tattgcaaag tgtaaaaaat aataagggag tggataatga
    44101 tgagtagtaa tataactatt gaaatggttg attcagtctt tgaaagagtt ccaggagcaa
    44161 cttatgcaga agcaaaagaa gcattagtaa tgtgcaatgg agatgtaatt gaggcagtaa
    44221 tatatttaga aaataaaaaa aatacttgta attgtaaagc aaaatctgct aaagaaacta
    44281 tggaagaagt atttggcaaa gatggagaaa atataaagtt tcaattaaaa gagcttctta
    44341 gaaaaagtag tgtagtgaga ataatagttg aaaaagatgg aaaaactatg atgaacattc
    44401 ctttaacagt tggagttgtt ggattagctt ttgtaccatt agctacttta gttggattat
    44461 cagctgctgt tataagtaaa tatcgtataa aagttcaaaa tgaagaagat ggagaaattg
    44521 tagatttagg tgaattgaat gaagaaaaac ttcacgtttt gaagaatatg ataactaatg
    44581 cagcaaaaga tgtgaaggat gtagtagttg acaaaaaaga agatgacaaa gatgtaactg
    44641 ctgaccttat gaaagaagaa tctgaaaaga ataaagaaga cgaagacgaa aataaataga
    44701 tataaagtga cttttaaaaa atgaagagaa gataattata agaattatca ctaacattga
    44761 caaattttat agaatttgat attctaaaag ttgtagaaga actattaaaa tagcctttcg
    44821 tgtagacgag aggctttatt gttataggag ggatttttat gaattttcaa gagatgatac
    44881 tggcgttgca aaaatactgg tcaaagcaag gttgtatcat gatgcaacct tatgatattg
    44941 aaaaaggtgc aggaactatg aatcctaata cttttttaag atcattagga cctgagcctt
    45001 ggcaagtatg ttatgtagag ccttcaagaa gaccagcaga tggaagatat ggtgaaaacc
    45061 caaacagact ataccaacat catcaattcc aagttatact aaaaccttct ccagataata
    45121 tacaagaatt atatttggaa agtttaaaag agattggaat tgacccaagt gagcatgaca
    45181 taagatttgt tgaagataac tgggaagctg ctacagttgg tgcttgggga cttggatggg
    45241 aagtttggct agatggtatg gaaataactc aatttacgta ttttcaacaa gtaggtaata
    45301 ttgaatgtga gcttgagaca ggagaaatta catatggatt agaaagatta gctatgtaca
    45361 ttcaagaagt tgatagtgtt tatgatttaa aatggaatga taaaataact tatggtgaag
    45421 tatttaataa agctgagtat gaaaactcaa tgtacgcatt tgaattatgt gatgcagata
    45481 tgttgtttaa tctgtttgat atatatgaaa aagaagcatt aagacttatg gaaaatggac
    45541 ttgtaatacc ttcatatgac tatgtgttaa aatgctctca tgcatttaat acattggatg
    45601 caagaggtgc aataggagtt agtcaaagag cttcatttat aggtagagtt agaaatatgg
    45661 caaagactgt tgcagaaact tttgtaaaac aaagagaaga aatgggattc cctcttttaa
    45721 aggacggtga caaataatga aaaattatct tttatttgaa atcggtgttg aagaaatgcc
    45781 atcaagattt gtaggcagta cactagaaca attaaaaaac aatcttagta aattatttga
    45841 tgaaaataga atagcttttg ataaaataaa tgcgtatggg acaccaagaa gacttacttt
    45901 agtagtcgaa ggattgagtg atagacaaaa tgatttagaa gaggaaataa agggtcctgc
    45961 taagaaaatt gctgttgatg cagaaaataa tttaacaaaa ccagcattag gatttataaa
    46021 aagcaaagga ttaagcgaat ctgacatata ttttaaacaa gttggaaaag aagaatatat
    46081 atttgcgact ataaagcaag atggaaaaga aacttcagaa gtactaaaag acatattacc
    46141 agagactata aaatctgtag tattcccaaa agctatgcgt tggggaggaa aaaatcttaa
    46201 atttgcaaga cctataagat ggatagttac attgttaaat gataaagtat tagagtttga
    46261 tttagaagga ataatatctt ctaatgtaac aaaaggtcat agatttttag gtgaaagtac
    46321 aatagaagtt gattctatag aagattattt tgaaaaatta gaaaaaaatt atatagtttt
    46381 agaccaaaac aagagaaaag aaatgataag aaaacaatgt atagaagtag caaattcttt
    46441 aggtggagaa gttgaatttg atgaagattt acttgaagag gtaacttatt tagttgaata
    46501 tccaactgct ttttatggag aatttgatgt ggattatata aaactaccaa aagaagttgt
    46561 tataactcct atgaaacaac atcaaagata ttttccagta cttaaagaag gaaaactgtt
    46621 acctaacttt atagctgtta gaaatggtga ttcttacaga atagacaatg ttaaagctgg
    46681 aaatgaaaaa gttttagaag caagacttgc agatgcttta tttttctata gagaagatac
    46741 taagagaaac ttagaaagct acatagaaaa attaaaaact gttgtattcc aagtaaaact
    46801 aggaactatt tacgacaaaa ctttaagact tgaaaaatta agtgctgata tacttgatag
    46861 tttaggttta gtagatgaaa aggcagatac tataagggct gctaaattat ctaaggcaga
    46921 tttagttact ggaatggtag gagaatttga tgaattacaa ggttttatgg gaaaagaata
    46981 cgcaaaagtt ggaggagaga atgatgttgt aagtgaagct atttttgaac attatcttcc
    47041 aagatttgca ggagatatac ttcctaagac taatgcaggg gttgccttat caatagctga
    47101 taagttggat tcaatagcag gtttctttgc tataggaata caaccaacag gttctcaaga
    47161 cccatatgct ttaagaagac aagctttagg tatacttagt atacttatgg acagaaaagt
    47221 tgctgtggat ataaaagtac ttattgaaca tgctttagat aattattctg aattagattt
    47281 taataaagaa gaagttgttg agcaaataat gagtttcttc aatgagagag taaaaaatct
    47341 atttaaagat ttaggaataa gatatgatgt tgttgatgct gttttaagtt ctgatataaa
    47401 tgatgtttct gatatgcata tgagagcttt agagcttaat aattggctag aaaaagatga
    47461 attagtggag atgcttactg catttaatag agtttctact ctagctcaaa aagcagaaag
    47521 tgataaaatt gatgaatctt tattaaaaga agatgctgaa attaaacttt acaatgaatt
    47581 taaacaagta aaggtaaaag ttaatgaatt attaaaggat aagaaatatg gtattgcttt
    47641 agatgaattt gcatctttaa gaccagtagt tgataattta tttgatacag ttatggttat
    47701 ggataatgat gaagctatta agaacaatag acttgcttta cttaaacaag tatatgatac
    47761 tatgctagaa atttgtgatt tatctaaaat tgtttataaa taaaatttaa actatttata
    47821 agaaataagg ctgtctcaaa atagagatag tcttattttt cattgttgag atgatgaaaa
    47881 tatatttgat ttcatagaaa aagagtgtct cataaactaa aaagttcatt ttgagacacc
    47941 catgtttttt gtaagtaaat ataataaatt atattttata cttatcagtt attttgttta
    48001 aaatattatc atttgaattt acccattcat aaaaattgcc tgatgatgcg tccgatattt
    48061 tataaagcat ttcaggaata tcactagctt taaaatctcc aagtgatttt cccaatctag
    48121 tcttacctat ttcaaaactg cctccaacaa ataattcaaa tgcatctgta gatataccat
    48181 caacattctt ctttttacca cataaaccta tgctaccaac ttgatgaaca ccacatgaat
    48241 ttaaacatcc tgatatatat aacttaggtg ctttatttaa aatttcttca ttattttgta
    48301 atctaaaata atctatgatt tcatgtaaca ttttctgact attttgtatg cccatttgac
    48361 aaattggaac acctatgcaa gatatacttt gttctaattg agaattacaa ctaatagttt
    48421 tacttatttc taatattctt tttgcttcat ttccatttaa atttaaaata tataatccct
    48481 ctgtcattcc tagtctaatc ataggatttt caacattatc taattcttta agtaaagtac
    48541 ttaaatcctt tgttaacaaa atacctccaa ctggatgtat atatacagtg tataaaccat
    48601 cctgtttttg aggtattagt ctagaatctt gtatatctat tttaactcca ggtttagagt
    48661 aatctatttc ttcaatattg agttcaagac taccattttc tttttctaat ttatagtatt
    48721 ctttaaattt ttctaagaat agttcttcac caagtgattc gaccatatat ctaaccctag
    48781 ctctattttt attttcataa tttccataat ccataaagaa tttaatcata ccttcaacac
    48841 aatataaggc gtctttcggc tttataagtt catcaagttc taaagcaact ttgggctgtt
    48901 tacctagacc tccacctaag aaaactctga aatatggttc atcatttttc aaagtagcaa
    48961 caaatcctaa gtcttgtata ctacaatgag cagtatctgt gtaacaatta gaataagaaa
    49021 cttttaattt tctaggtaaa tggtatgtag tagcattttt gataaaatat ttatcagtag
    49081 caactgcata tggagtaaca tcaaagactt cactagggtc aactccagat aatggagata
    49141 atccgacatt tctaggataa ttaccaccac cacctctagt gaatatatca ttttttattc
    49201 cttcttccat gatatcaaca attgaattta tagataaatc gtgtaattgt atagcttgtc
    49261 tagttgttaa gtgtatctta tctaagttgt gtttaatagc tatttcatag attttattaa
    49321 gttgatactg agataaaact ccagcagata ctctaagtct tatcataaaa gattttttgt
    49381 ctctttgagc ataaacacca tatccaccag aaaatccctt atattgcatt ttatttattt
    49441 ctccatttaa aaattttgaa gaatattctt taaaattagg tagttcattt aacaatatct
    49501 ttttaaaatt atccattatt tcacctcatt gttagtcttg tataaaaata tatctattgt
    49561 ataatagata tatatattat tatctagtca ggataaaaat ataacagaat aatt
//
LOCUS       DAENCV010000014        49356 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAENCV010000014 DAENCV010000000
VERSION     DAENCV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.014"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            303..2063
                     /locus_tag="KQD82_000740"
     CDS             303..2063
                     /locus_tag="KQD82_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379230.1"
                     /translation="MDRFEVLKYLKFNVWHSEWPLELLKGAITKDNATGNLLLQLKMC
                     NISDKEIKSAYFTIECFNDAGDLIDNNLKYVYQDINIKPHECLGDDIAIKINDNKTRA
                     INIILEQVVFSDDKVHKYEREEGLIIPKLDKIELLNIELLEELKKDYEEYDFQYEIKY
                     MPNIIEKKAWSCCCGKINKLEKDKCDNCKRSKILQIKKINKNYLESIYKKSLKKKKEI
                     EIKLEKDNRHKLKNINKNNRRTSILLISILLIFIIFIGKNIINKHAFLDEIKYKNEYS
                     KKISVEKDNVYMLKNNGKVTHRKVNGESSSIPGWENIISIASYDNSIIGIKKDGTVVS
                     KGLGNSDSDNISKWRDIIDIECDSGTFIGLKKDGTVVATGNNDYGQCNVNNWFDVVSI
                     EITKSTIEYMKNTTVIGIKKDGKVLLTGYDSLNHRKETDKWRDISKIKCGSYGIFGIK
                     NNGTVVVSGSAYDIKNWKDIVDIHIGLCGIVGIKRDGTIISTDEKFNEIIKNWKDIIS
                     AEIIDNHEGISIVGINSNGDILTSSRGQNLDNIKRLEDVEYINFDDSIDNFLVGIRKD
                     NVIFIRGINNTINEEYNEEI"
     gene            2097..2453
                     /locus_tag="KQD82_000741"
     CDS             2097..2453
                     /locus_tag="KQD82_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379231.1"
                     /translation="MKNYIARCLSNMGVLLAGIQVLAGIIIMCQKVEVPYDYLSDTYE
                     QYSPIMIGIGAGVIVSGIVICLVLKGLAELVENSADQTQYQKEILMLLKNNNVNEKIN
                     EVKIEQQFTNEELPKL"
     gene            2662..3375
                     /locus_tag="KQD82_000742"
     CDS             2662..3375
                     /locus_tag="KQD82_000742"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379232.1"
                     /translation="MIITICENDDMQRDIIYDFVKIFLTKIKVKHYDILMFKNGEELL
                     EEYPNKVDLILMDINLGGRNGFDISKDIRKFDKDARIIFVTVSEKYAIKCYEINAFRY
                     MIKPIYYKEFEKYMLDFAIDWRKREAIITIKRKKQIIKIEEHNILFVEIVNRKLRVVT
                     KNEEHIINDKMDMFEEKLKEDIFFRCHKSYLINLLEVTNFNYNFVYLTSEKIKIKVAP
                     KKTKKLKEKLANFFREIIW"
     gene            complement(3839..4192)
                     /locus_tag="KQD82_000743"
     CDS             complement(3839..4192)
                     /locus_tag="KQD82_000743"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379233.1"
                     /translation="MNENLKELRIKLGLSQKEFAEKLLVSDATISRLEKGERKLTDRF
                     IFQICEVFNVNEDWLRNGNGEMFKNIDDIELAAMMGKTFSSNDEFLKKVFLTFAELTD
                     DEREVIQKVIDKLSK"
     gene            4372..4542
                     /locus_tag="KQD82_000744"
     CDS             4372..4542
                     /locus_tag="KQD82_000744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379234.1"
                     /translation="MYNNNDKKLINLFESFKKIDYTEQSLVYFLSLSFIVNRKCHKDA
                     NLYSRRGECNYE"
     gene            4535..5341
                     /locus_tag="KQD82_000745"
     CDS             4535..5341
                     /locus_tag="KQD82_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG4379235.1"
                     /translation="MNNLNIKGKQKFMDLEIPIIEGGFGEGKKCITDKTISEIHNMEI
                     KHVRELINRNIRRFINAIDFIDLKVVVDNDYNLEVLELLSYRKMQISKANNIYLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMREQLIDSYMIQDSIERAKRWIEEEKERLLL
                     KETIQVQQPKVEYHDKVLNPDKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGR
                     IKCWKPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            5405..5629
                     /locus_tag="KQD82_000746"
     CDS             5405..5629
                     /locus_tag="KQD82_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379236.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKLDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            5653..6189
                     /locus_tag="KQD82_000747"
     CDS             5653..6189
                     /locus_tag="KQD82_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379237.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLSDKIDTIKELKNTLAKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKQKENQGKSRFKKNKK"
     gene            6198..6338
                     /locus_tag="KQD82_000748"
     CDS             6198..6338
                     /locus_tag="KQD82_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379238.1"
                     /translation="MEEYQFNLIKKLCAERKIHKAWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            6349..6582
                     /locus_tag="KQD82_000749"
     CDS             6349..6582
                     /locus_tag="KQD82_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379239.1"
                     /translation="MENQTELTEKDIENTLSALNIIKYICIKSKHCKNCPLGYMKNLD
                     EHNCMLYALIDRGLLPCDWNAKKVTRLLDAIVE"
     gene            6606..7028
                     /locus_tag="KQD82_000750"
     CDS             6606..7028
                     /locus_tag="KQD82_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG4379240.1"
                     /translation="MIHELKILPKYYEAVSNGSKSFEIRFNDRDFKIGDTLKLQEYID
                     GVYTGRSLQREVSYILNDSNYIKNGYIILGLINNTNHYMFKVVRDYKKENYLICRQGK
                     VFIGYILGNFACCKESLEQFTIPLEYIEKISRVEFKPL"
     gene            7221..7763
                     /locus_tag="KQD82_000751"
     CDS             7221..7763
                     /locus_tag="KQD82_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBG4379241.1"
                     /translation="MSKKDKLSIFISLILRHKPETIGIKLDDYGYADVNELIEKINNT
                     GRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSINVNVELRELEPPKYLYHGTA
                     TRFLDNIKNEGIIKQSRLYVHLSRDIDIAVKVGKRHGTPVILKINTGKMYENGYKFYL
                     SENNVWLCEYIPFKYVEIFE"
     gene            7813..8181
                     /locus_tag="KQD82_000752"
     CDS             7813..8181
                     /locus_tag="KQD82_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379242.1"
                     /translation="MSRCKDCFYFEKNKIEESGICNKKEKEVYKENRKCEFYESKKWK
                     SMNIDEFWIEMERWTNEKVNRYIIYFSEHDSIQIDIVQDINRPNNPTDDEVIEEYVEN
                     NYGYVKYEVIDLGTVENVKL"
     gene            8201..8977
                     /locus_tag="KQD82_000753"
     CDS             8201..8977
                     /locus_tag="KQD82_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379243.1"
                     /translation="MENIELTPRDYFEDIKNRKQKNTNEKLDIIYENSLILLNKYIVT
                     GQKKGAKKLMFLLECIEKERNVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKEISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGRQDILKHIITPKSLDELKKELDSLEKNN
                     DTYKQLANIKPLPTTKNSSVEPPKLNIFKKIKNRVFKNEK"
     gene            8967..9371
                     /locus_tag="KQD82_000754"
     CDS             8967..9371
                     /locus_tag="KQD82_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379244.1"
                     /translation="MKSNVDLTENRDFRAVRGRGINILGIEDAPNRMLNGFSDFGIGR
                     LYGKNLEFEFNKNEVFLTGNANDREEKKFLILCEENKVCVRCGDELKERWDKVSIELC
                     NKCYEMLDVEYKNKKIIRELFFRDRDVNIELL"
     gene            9409..9798
                     /locus_tag="KQD82_000755"
     CDS             9409..9798
                     /locus_tag="KQD82_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379245.1"
                     /translation="MKDVWLYSWDDECFRSDEYESKEEAVEAAKEELKRLGEIKRWVY
                     IGKKEEADIPDIDAKDALECIQNRIDDEYGEYGEDWFENIRAEDILILESRISEVFKK
                     WIDDFKYKPYWFAVRDIEEIELKNVVN"
     gene            9858..10178
                     /locus_tag="KQD82_000756"
     CDS             9858..10178
                     /locus_tag="KQD82_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379246.1"
                     /translation="MFIKGFRCICRHKFSRIKDCYLITTKSQQHTCKIWVCKYCGQIV
                     AGSTPVYHPNMKVNEYCVKCELLACSNNYEGYCNSKDKEIEFKSICLEGNKEILQCKN
                     FDLE"
     gene            10209..10394
                     /locus_tag="KQD82_000757"
     CDS             10209..10394
                     /locus_tag="KQD82_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379247.1"
                     /translation="MKINVHVKTNKIGSDCKLEFEIDDDDLKDMSDEEREGYIDKIAW
                     DYVFENFIDWNWYEVED"
     gene            10449..10850
                     /locus_tag="KQD82_000758"
     CDS             10449..10850
                     /locus_tag="KQD82_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HBG4379248.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTGHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIKLGITYKTVDKIMPGLII"
     gene            10952..11455
                     /locus_tag="KQD82_000759"
     CDS             10952..11455
                     /locus_tag="KQD82_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379249.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDEILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            11515..13740
                     /locus_tag="KQD82_000760"
     CDS             11515..13740
                     /locus_tag="KQD82_000760"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG4379250.1"
                     /translation="MKNVIKRDGRIVPFDEKKIINAIEKSFLSLRNKGKSEVFDRELA
                     TLIAKKIKNKPCEKMHIEDIQDIIEFSLMASKKKDVAKEYITYREKRNIARGRKTYND
                     YMSIVNTESNDITKENANMNADTPAGMMMKFASEATKTFTDHVLLSEEAKEAMMDNYI
                     HIHDKDYYPTKSLTCIQHPLDKIFNNGFRAGHGSSRPTKRIETSSILGCISLETVQNE
                     MHGGQAIPAFDFYNAPSVRKTFIEEIKNIEIKDDIDLSHLYNIEIEDYIIKDLKELDS
                     EKRNVQHAINRTVWRVHQSMESFIHNMNTIHSRGGNQVVFSSINYGTDTSAEGRCIIR
                     EILKSTYEGVGNGETPIFPIQIWKVKEGVNYNPTDRNYDLYELACKVSAKRFFPNFLN
                     LDATFNKHVKWKMCDPNRYIYEVATMGCRTRVFEDLHGEKTSIGRGNLSFTTLNLVKM
                     ALEAKEKYPNNEANRVAEFIFLLDKYSDVVARQLYDRYLFQCTAKAKQFPLLMSGLWK
                     NSENLNPNDDVSEVLKHGTLGVGFIGLAECLIALIGEHHGESEKAQKLGLEIISLLKS
                     KVEKYKKKYNLNYSVLGTPAEGLSGKFTQKDKKIFGEIKNITDKDYYTNSSHVPVWYN
                     CNVEHKAKIEAPYHELEGGGHIFYVELDGDATHNPKAIMKVVDLMKKYNMGYGSVNHT
                     RSRCMNCGFENADSELKECPKCGSNSINIIQRITGYLVGTTDSWNNAKLAELKDRVTH
                     G"
     gene            13733..14230
                     /gene="nrdG"
                     /locus_tag="KQD82_000761"
     CDS             13733..14230
                     /gene="nrdG"
                     /locus_tag="KQD82_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG4379251.1"
                     /translation="MDKDYIYILDIKHDTIVDGEGFRTSIYCSGCNHMCRGCHNPQSW
                     DIKNGTLTKVLDIYKEIISNKFSDVTFSGGDPLLQLQGFVHLAKLIKKNTNKTIWCYT
                     GFKFEELITDNEKLELLKLIDVLVDGKFEEDKKSLELIFKGSSNQRIIDVQKTLYEKQ
                     IVLYE"
     gene            14431..14838
                     /locus_tag="KQD82_000762"
     CDS             14431..14838
                     /locus_tag="KQD82_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379252.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNCLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            14859..15170
                     /locus_tag="KQD82_000763"
                     /pseudo
     CDS             14859..15170
                     /locus_tag="KQD82_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15261..15611
                     /gene="tnpA"
                     /locus_tag="KQD82_000764"
                     /pseudo
     CDS             15261..15611
                     /gene="tnpA"
                     /locus_tag="KQD82_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            15629..17068
                     /locus_tag="KQD82_000765"
     CDS             15629..17068
                     /locus_tag="KQD82_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4379253.1"
                     /translation="MSNYILTLQLKTEKFQEDIICNAFDKYRKVYNSCLSELFKRYNH
                     MIESKEHQANCKYKGKDRNKIFSEINKKYNLTEYSLHKFVKFIGKYYKLHSAITQKIA
                     TRCFNAFNKYIYHQAKRVNYIRYNELTSIEGKQNSTGISYKDGIIKFNKMTIPVIIKN
                     NDNYAQRAIQDKIKYCRILKKEIKGKIKWYVQLVLEGIPPKKTTNQGKIKGQIGLGNV
                     GIDIGTQTIAISSKCDVKLLELAPNVNNIDRQIKLIQRKMDRSKRATNLNKFNDNGTI
                     KQGNRDKWIFSNHYLKLKRLRKELYRKQAEIRKQDHYILINKLLNLGNKFYVETMSYK
                     RLQTRVKDTTINEKTGRINKKKRFGKSLANKAPSMFLTMLDNKLKYNNERLYKIDTAK
                     CRASQYNHFSDEYNKKELKDRWNNGMDIQRDCYSAFLIMNVNEDLKSINRELCVKTYD
                     NFKILHDKEINRLKKLKLNGYKLISSMGI"
     gene            17317..17493
                     /locus_tag="KQD82_000766"
     CDS             17317..17493
                     /locus_tag="KQD82_000766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379254.1"
                     /translation="MYDYGMSLSSIMKVILNHQEDIEKLKEEEVAEFIKNKLENEHDC
                     DELKIISEVFNEIY"
     gene            17480..17590
                     /locus_tag="KQD82_000767"
     CDS             17480..17590
                     /locus_tag="KQD82_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBG4379255.1"
                     /translation="MKYIKHVDNGVMRAIGIDNYKPLLNIIKEKVNLESN"
     gene            17619..18086
                     /locus_tag="KQD82_000768"
     CDS             17619..18086
                     /locus_tag="KQD82_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379256.1"
                     /translation="MGLDLGFYSVKTKVTDLDIYLNKTRGEKYKELNNDEEEFYKFEE
                     LLDIDISLEDWSVIRMIYKAKWEKGYESYEDEAILITKEDLEDYIIPFLKQKDINSKI
                     YNKDKEYYYKNDKKVTVDDRYTQEFWDLVLKEFQKMLNIIDFEKHSLIIMYWY"
     gene            18122..18502
                     /locus_tag="KQD82_000769"
     CDS             18122..18502
                     /locus_tag="KQD82_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HBG4379257.1"
                     /translation="MKKMYIAINSDLNMSPGKVGAQVAHAVYDYLYNKILDTVSCSYE
                     TEIIAHELIDLKCDLTSFKNNGDTICILKAKEAQLLKFKEKGYLTIVDRGLTEIPKNS
                     ITCVNLGIFNEDEIPSTIKRLRLY"
     gene            18518..18673
                     /locus_tag="KQD82_000770"
     CDS             18518..18673
                     /locus_tag="KQD82_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379258.1"
                     /translation="MYSKGDVVKVWCYQCERIEFQEFVGYFNSYFDNYKLKKRWKCSK
                     CDVINNF"
     gene            18700..19146
                     /locus_tag="KQD82_000771"
     CDS             18700..19146
                     /locus_tag="KQD82_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379259.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGGFYLDNIHEIIGGFALSYIG
                     KCKKADYLHIQIHKRVDEIQRLHDYKDITNVKIEYENGSVEDIYVPYKQEGDFLGLDN
                     LYQTSFISKRGYLHIVISKDKTVEEVFNEEFEDDEGVEFSFNMYGI"
     gene            19424..19588
                     /locus_tag="KQD82_000772"
     CDS             19424..19588
                     /locus_tag="KQD82_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379260.1"
                     /translation="MTDKEMEEYIINLIKDKIKEEYGENRLPEILMEKFIKLERKGSV
                     TVDVQDLIEG"
     gene            19632..20885
                     /locus_tag="KQD82_000773"
     CDS             19632..20885
                     /locus_tag="KQD82_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBG4379261.1"
                     /translation="MEVQGKYNKAKIFTENVDSITIGQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISDKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRDYIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVHTGSRNLGKQIADYYQDKAIKYCIEKYKKSYDDAKEFLISTLKKDNKEHLINKY
                     LKDLSDRKLNKPHDDLCYLENDLMEDYLHDMSIAQKYAAANRMVIIADILFKYNSLDV
                     NYDHFIRDIKCDVIECIHNYIDMDSKILRKGAISANKDETVIIPVNMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLDEFEESMKEIFTTCVGQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPVYNFKSN"
     gene            20925..21326
                     /locus_tag="KQD82_000774"
     CDS             20925..21326
                     /locus_tag="KQD82_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379262.1"
                     /translation="MGMYTEFVCAIELKKDTPKEVINILNNMIEGEDRYDITPPHPFF
                     ECRRWRWLFIMDSYSFSGRSNTMFEYDDIAKTYYLTIRSNLKNYDDEINKFLNWIKPY
                     IQIHCEDDEFLGYSRYEECRNPELIYYNEIQ"
     gene            21353..21586
                     /locus_tag="KQD82_000775"
     CDS             21353..21586
                     /locus_tag="KQD82_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379263.1"
                     /translation="MKFEDNMIKSFREVRVGDIILAGNNKYLIISILNYFGYVNLDTG
                     DCSSGFRSIEELMKQFDKQTRFIQNDRFKLILD"
     gene            21629..22039
                     /locus_tag="KQD82_000776"
     CDS             21629..22039
                     /locus_tag="KQD82_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379264.1"
                     /translation="MCKFCESIIDNTKDILLTSRSRLLEDNTCEIIAEDNCNNCYDGC
                     YEYFKLNGYKIRGNTYVNVDYYKKIRKIIIAPCSESVHINYCPYCGKQVSKDIKDFNC
                     IPEHIVDVRYKNGDVYDYDMEKSVQEVMDNANNN"
     gene            22070..22243
                     /locus_tag="KQD82_000777"
     CDS             22070..22243
                     /locus_tag="KQD82_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379265.1"
                     /translation="MNLWSKIEEPVMRAVSGIVFNIWGNVCFSIYYIEENKQIELEIY
                     DRPYRYEGKKNSC"
     gene            22353..22496
                     /locus_tag="KQD82_000778"
     CDS             22353..22496
                     /locus_tag="KQD82_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379266.1"
                     /translation="MNKGIKNYITAVNYLLDNLEYMMEDDEFNNEKLQKRIDVIRKFE
                     REL"
     gene            22498..22803
                     /locus_tag="KQD82_000779"
     CDS             22498..22803
                     /locus_tag="KQD82_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379267.1"
                     /translation="MGKIKNGEIDTEDYRLFKNIQKEKDRVYSKYINEEYTMEEFINY
                     LRLEDKDYTTEILERYARYIDENLDNRCPYCVYNICSEHDNCFDGILENLKEKIKIK"
     gene            22829..23182
                     /locus_tag="KQD82_000780"
     CDS             22829..23182
                     /locus_tag="KQD82_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379268.1"
                     /translation="MKLINGDRCSGKTTTLIKYAYENNALILCYSCGNLNYIIEKAKE
                     LKLDIIIPRVFKTYIESNHKIEYMKTINGNIINDTSNIRLVIDEIDCCLESIIREGID
                     CVTGTIQIENLNNHI"
     gene            23262..23546
                     /locus_tag="KQD82_000781"
     CDS             23262..23546
                     /locus_tag="KQD82_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HBG4379269.1"
                     /translation="MKKILLIGALGTMLIGMSGCTGIQSSMKDAESDVSGLNRVVNVY
                     SDNGEVLKTYTSKSMRVKDGDGGTITLDFDGKRVIICNAHVVIEENQSNK"
     gene            23558..23725
                     /locus_tag="KQD82_000782"
     CDS             23558..23725
                     /locus_tag="KQD82_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379270.1"
                     /translation="MFKVLKTILIIIWVVDILNFPQFQFLDTTYPINTLAWLLIWILI
                     PSSSIYIDKKE"
     gene            23903..24055
                     /locus_tag="KQD82_000783"
     CDS             23903..24055
                     /locus_tag="KQD82_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379271.1"
                     /translation="MKITISIEEAMDIIKQHYENKGIEIKDLSFNLEKTGMVRNIKFK
                     GEIILK"
     gene            24103..24705
                     /locus_tag="KQD82_000784"
     CDS             24103..24705
                     /locus_tag="KQD82_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379272.1"
                     /translation="MILISSTNRSNARDFHVSDYYRTPITKIKEFLTEFSRYENILNP
                     NIKILDPCAGGDNTNPMSYPTAIQEFLNQDISIDTVDIRDDSRANIKQDYLKFQPKEK
                     YDVIITNPPFNLSLDIINKALNDVREGGFVIMLLRLNYLGGKVRQRLWGNNMPKYIFV
                     HNRRMSFTDDGKTDSIEYAHFVWQKGYNPKFSQLKVLISQ"
     gene            24720..24929
                     /locus_tag="KQD82_000785"
     CDS             24720..24929
                     /locus_tag="KQD82_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379273.1"
                     /translation="MKVKYVVFEWEITSKNDGQKHFINFRDLIKLYGVSSRECIRAKD
                     YYERDGLDLKDIKFLYPRDDGKYKL"
     gene            24984..25229
                     /locus_tag="KQD82_000786"
     CDS             24984..25229
                     /locus_tag="KQD82_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379274.1"
                     /translation="MDIKIDMCLLEMEINRRRCEYAARTGRYPNSLILWEGYYSLLNI
                     QYIEDKTRGYITKFNGMDVIKCNDFLKIELFEKNINN"
     gene            25258..28329
                     /locus_tag="KQD82_000787"
     CDS             25258..28329
                     /locus_tag="KQD82_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG4379275.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNVLTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQATRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKICELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCNINTMRKYSNKETEKIFKEVDTKELCSYLESQIENTDI
                     PTNEKIQAHFEFVGSCNIKDSNSNPRYCLVIDIDTKYSPKVTLYNISSGNIKIFKVSK
                     PIFEDNKIDVYDLVYLKSIKEKAKSKKVDGKWIRSNTETEWWIQEYWKIEN"
     gene            28432..28683
                     /locus_tag="KQD82_000788"
     CDS             28432..28683
                     /locus_tag="KQD82_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379276.1"
                     /translation="MISILIDEQRYYSLLEEKKDLSMRIDELKEENAFLKQENEEYEE
                     ILNEWECVRKEIVATCKKKEILGEKPSELEMVLQKIEDI"
     gene            28683..29003
                     /locus_tag="KQD82_000789"
     CDS             28683..29003
                     /locus_tag="KQD82_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379277.1"
                     /translation="MSVNKIYEKKEYIIIKSKSGYIVYNTNKEFKEGHTHLKSYNAAK
                     TAIDLVIKRRLPKSYSIYFLTSLMRISNDVQYIKHISDIVYIRNNKGLKEKYINSKNI
                     KKNY"
     gene            29078..30199
                     /locus_tag="KQD82_000790"
     CDS             29078..30199
                     /locus_tag="KQD82_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HBG4379278.1"
                     /translation="MKSYKEKQFLVFEFENGKNVKYNLATGEYVGKSGRIVKDVCTQL
                     RGYNLHEVINSFEDENYKCFLNFVDERVNRSKSSRAWGRRVERIRNIGSFLNEINNYS
                     EYEQLFSAGLKKIIYPIHCDIKSIPKGLISICRNYSITLSDRLIQGYSKNPNLYNNLL
                     SLELNSIRKTNLISILDSGYDRMIFDRLIDIYKYKPQSLINYIDNLMTYEALNGLEST
                     LNELYDYVVMMSVISDKYEKYPRHFLTTHKIASRNYNRLKTQFEEEVFNKRIDKSLEY
                     SYQDYKFIYPKNTNEIKDEAVQQNNCVSSYIQNVIDGKCHILFMRKKDNPSKSLVTIE
                     VRNGKIVQAKGKYNRDVNEREQEAIDKYNNRIERMKKIC"
     gene            30193..30624
                     /locus_tag="KQD82_000791"
     CDS             30193..30624
                     /locus_tag="KQD82_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379279.1"
                     /translation="MLIERTKIRLMKEIQGFEMLKIGDIFVITSVGNNGAIHFKTDYG
                     IGFMNYSEFEAYFEIVQQKKKYEWGAWSIRGDFSGAYLYRTNGKKVEVRKGNFKASAT
                     CHDSDEFELNKGVELCLARIEVKKANKQVNLVLDKINNKEI"
     gene            30634..30789
                     /locus_tag="KQD82_000792"
     CDS             30634..30789
                     /locus_tag="KQD82_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379280.1"
                     /translation="MICKFKERCENKNRDCDYCCYNPNAYLEDAFEWNGEGRKPTDEE
                     LDDALEE"
     gene            30833..31393
                     /locus_tag="KQD82_000793"
     CDS             30833..31393
                     /locus_tag="KQD82_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379281.1"
                     /translation="MRWRNILVTSVLVMGSSIHIFANTKEMSTSRMDKNIVADTAKNI
                     NLMYKYEWEQHKNSSEYKLEIKRINLEKDLGIKIEKLIPVTWEVSYYTSLNCENSKYG
                     AITATGEKLQYGFVANNYLKFGTKILVDGNLKVVKDRGSNKYFGNSNAIDVFVPKLEG
                     ESDYKYYKRVNNMGRHYKEGYIIVEG"
     gene            31405..32073
                     /locus_tag="KQD82_000794"
     CDS             31405..32073
                     /locus_tag="KQD82_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379282.1"
                     /translation="MNVNGIKIPGIIDVSNVYVYGIKESIIASGYPMYEQQVIDMKKI
                     NISEKDLKRANQLGKSKQGSGHDCFSKGIIIQMDLQWSEYMWRQWDRYHHNDYVSSQS
                     KMHRLSKFNLDDMCNKYVDKNTINFLNELIHEYNNFEELKLNNSNNIILRNGEKVEVT
                     KETLWKIIISNCPSGLMLTARVTLNYLQIKSMINQRENHKMEEWRILCDFFKTLPKYK
                     ELMM"
     gene            32119..33012
                     /locus_tag="KQD82_000795"
     CDS             32119..33012
                     /locus_tag="KQD82_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379283.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEIKGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVVSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDKIFSDKNYPFTDKNIEAMREKIKEKDKKE
                     KQRDIDKNGKTIEIRDDDVPF"
     gene            33052..33609
                     /locus_tag="KQD82_000796"
     CDS             33052..33609
                     /locus_tag="KQD82_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG4379284.1"
                     /translation="MEVKEKTMNDGFKKVVNEEIKTYISNLYSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKGKSEEENSTIKKSRV"
     gene            33670..34221
                     /locus_tag="KQD82_000797"
     CDS             33670..34221
                     /locus_tag="KQD82_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379285.1"
                     /translation="MNCKCRFCKKKLNTNDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVINNQKNKMLQEIIKGGYTREELYDCIIEKKDEVIELL
                     NYRKDIEEEYPKLCYVFTILKGSIRDITIKNKQMKYEKENERIYKEVEKSEEYYEIIA
                     PKKVIYNKRQSLFDKVKGVYQDE"
     gene            34262..35788
                     /locus_tag="KQD82_000798"
     CDS             34262..35788
                     /locus_tag="KQD82_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379286.1"
                     /translation="MVLGTMLKSLTLFSEYKISESDFIIDKVKFFFSLGRIMSKTHNE
                     LDEISVAKFVSSNKLKSEYERYGGWNSLSSAMEYGKETNIAAYIDDLAKNNLLIALDK
                     RGFNVIKEMEHNGLKFIPFEIFQSMKCREVEEFYEGLISSCSVNSIKNNMKVENLLLT
                     KDDREKLKQKTEAGTPYNIIFEYTEKEIGLSNNEESKYIYSLPILSNRTNGIGKGGGI
                     NIIAGFSGIGKTTLLFFNYILAMIYRGEKIVIFANEQKSQYFKSMLVSFIAYNIFNYH
                     DLDRNKIDNGDFTDFEEDLMEKIEKFLRDRCFAENLKFIYMEEFEISEILRKSKELVT
                     HQGFTGIAVDTFKSEDSSDAHYTGKLIENSKLLDSFGNKYNVITMLSMQLLTAQENKS
                     SYLSAGDLSEAKAVKNVCGLLKLMRKVVNELELDSTNKKFFLKPYKLKYNKLKKTTER
                     EYIQFDAKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRLEEIGRCEKVYRGQLSY
                     "
     gene            35798..36859
                     /locus_tag="KQD82_000799"
     CDS             35798..36859
                     /locus_tag="KQD82_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379287.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYEFKDAINEIKDFFGISNQPMLRKGF
                     RIKKKVEQIHDIKDIQIELLPTPKKPYVYKTFQQVPIEEWENENISFEVLKTYQVYYN
                     LYENQIVIPHFCWHDRARPVGIRVRNLDEDKAKSFGKYIPLWYDNRCYNHRLSLNLYG
                     LNVNKKSIKNSKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILIDLGIEE
                     LIIAFDRQFKVKDDEEYVIWRDKIYKLVQDIKDVVNISVIWDKYNLLGYKDSPSDKGK
                     TIFEKLLKNRINIENFVKQ"
     gene            36964..37236
                     /locus_tag="KQD82_000800"
     CDS             36964..37236
                     /locus_tag="KQD82_000800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379288.1"
                     /translation="MIKFLIKNVHMGDKLYCINNIGNYKKGEIYTVSDTSFFGLFHKT
                     KGENCLPIECYFIHLPKKEELESNPKSMTKEEIEDELGYKIKIVSK"
     gene            37257..38108
                     /locus_tag="KQD82_000801"
     CDS             37257..38108
                     /locus_tag="KQD82_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379289.1"
                     /translation="MSIFNKKYGRNVKITEEEPPSPPPKQPIYNRDNIQYDKEMERKL
                     LDIDRLKIELEKEKKIEEERKKVERVKENEGKEWAWVEGYKCTDKNMKCKEFQYELNK
                     AYSMDGDDINIWNWGFHLCLDLGDVFQYYAPNLSNRFFKVKALVKKADKYKYGKEYGE
                     VTLLSGSVLRGHKINKIAAKEIIFLEELTFDKLKLHIQNSFPEVNTKSKWYAICKLGE
                     QEYYHRMFMNQMKKIRFSDTFSQLLFDECRNYCEILEILNKAIAYSKENLSKDMIIYL
                     LMKDIRK"
     gene            38132..38575
                     /locus_tag="KQD82_000802"
     CDS             38132..38575
                     /locus_tag="KQD82_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379290.1"
                     /translation="MDKINKIDLIKLKEMNIYDNYQLKKIASKMILHLRYEIMDFCGC
                     GSPEDISFMIKGVLTAIQNKEKNCNLEYTERCYIFEIEFNNVCGIIGSKNDLIQEFIL
                     NALNSYGLLEHGSSIWGSWLSDYGKQILCAFEIVGDCILDVSYLD"
     gene            38602..39261
                     /locus_tag="KQD82_000803"
     CDS             38602..39261
                     /locus_tag="KQD82_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBG4379291.1"
                     /translation="MIKYVMSDLHGMYDKFISMLEQINFNSNDHLYILGDVLDRGDKS
                     LEIIDYIRKYKNITLLKGNHELMFQESYTDVNSSFLWFYNGGKDTFYDLGMKSYEYKE
                     NFYKYIKNLPYLEIIDNFILVHAGLYLPNNYENLSIEQIIELQEEDICVWDRTILNTN
                     KHIKGYTIIIGHTPTQNIKDYEKAEIYKQEGIINIDCGACFDNGKLACLRLDDMKEFY
                     I"
     gene            39272..39799
                     /gene="yyaC"
                     /locus_tag="KQD82_000804"
     CDS             39272..39799
                     /gene="yyaC"
                     /locus_tag="KQD82_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG4379292.1"
                     /translation="MDILKISYNNLNAISKISEVLRDIVNEDTLIVCIGSDRVLADSL
                     APMIGSILEKSTIRNKIFGVLGDSIHALNLEQKIQAIKNNYPNSNIIAIDACISKISD
                     KGTIIISNKPVKPGLGVDKKLLEVGDYSIVGIIGRNKYDIYDTSDPELILDLADVISK
                     SLISILLEKEERMIV"
     gene            39796..40050
                     /locus_tag="KQD82_000805"
     CDS             39796..40050
                     /locus_tag="KQD82_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379293.1"
                     /translation="MIQEVRQEILLKNGLILYTGDLVEIKYKSDEDVIEHTCKGKIKE
                     AKELFIKLDTSKKYKTSERMIYSWELKEIKKVDDEDEKDN"
     gene            40034..40291
                     /locus_tag="KQD82_000806"
     CDS             40034..40291
                     /locus_tag="KQD82_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379294.1"
                     /translation="MKKIINSKYRALYLFIFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEEDAK"
     gene            40302..40511
                     /locus_tag="KQD82_000807"
     CDS             40302..40511
                     /locus_tag="KQD82_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379295.1"
                     /translation="MELKDYISKVELNDMPLTLDNKYMLNEISISEDSKIFAVKDTSG
                     ITSYYTEIEFNKLFRGINSILENII"
     gene            40586..41425
                     /locus_tag="KQD82_000808"
     CDS             40586..41425
                     /locus_tag="KQD82_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG4379296.1"
                     /translation="MEKELYSFSKLNAFNTCPFQYYLTYVKNLNREQSCYGYYGNELH
                     SLLEQLQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDRYYIEEYFEFDINGITMRGYIDLYYIIGNKIYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKIIEYVAFDMCKYMNNEKGVLIERNKIDSINDYERAIVKIKYTKELE
                     KQLIDFVVDTVTQIKQLDSNDESVWCKSDDKSNQFFCKTLCSHYNKGCKHKENKSFRK
                     SKS"
     gene            41463..41759
                     /locus_tag="KQD82_000809"
                     /pseudo
     CDS             41463..41759
                     /locus_tag="KQD82_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41776..42357
                     /locus_tag="KQD82_000810"
     CDS             41776..42357
                     /locus_tag="KQD82_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG4379297.1"
                     /translation="MDKLKNIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRN
                     YLEIINKTYYFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVD
                     ASGYKALEKYFTGTKTKLVPFFINADEDTIRKRLIDRGDNLKEIERRLVDDKIKFKEF
                     LNSENYIVIANNLSITNAVEQVKIHMKEGIQWS"
     gene            42348..42842
                     /locus_tag="KQD82_000811"
     CDS             42348..42842
                     /locus_tag="KQD82_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG4379298.1"
                     /translation="MVLALDVSMSSTGWAVINRNKNILKYGKIVTKKDKFKSEDERMC
                     YICNAIQELIITHNIQIVLIEDQFTSRNSKTILSLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLLNNGKAKKEEVAAYVRENIIDIGEYIDRTCKAKTSDIYDAIALGIAYLNKL
                     DTNR"
     gene            42866..43369
                     /locus_tag="KQD82_000812"
     CDS             42866..43369
                     /locus_tag="KQD82_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HBG4379299.1"
                     /translation="MSINVKIKKLNQNAIVPSYAKEGDSGIDLYTLKDTVIPAKSAVA
                     ISTGISLEIPYGYEMQVRPRSGISLKGMFFTRYVTIYDKVQPKKQIRKIFVAHPTVRL
                     GTVDSGYRGEIKIITYNEEDFDILIPKHTKLAQGVFQQVPRAILKVVDELTSSERGSN
                     GFGSTGI"
     gene            43534..43707
                     /locus_tag="KQD82_000813"
     CDS             43534..43707
                     /locus_tag="KQD82_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379300.1"
                     /translation="MYRYDDYVNLINDLELPKEIRNKCVRVIMTLENMQDIMVVHKFK
                     TYVVNEKYIEKVA"
     gene            43827..44576
                     /locus_tag="KQD82_000814"
     CDS             43827..44576
                     /locus_tag="KQD82_000814"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HBG4379301.1"
                     /translation="MFKEIFEKVFMSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDF
                     ILSRLGKDYIIIKDIIVPGSNKTTQIDSIVISMYGIFVIECKNFSGYIYGNDKDKVWT
                     QIVGKTKNTFYNPVRQNYAHIKAIENIIGNKYNIYSIIVFSDKATLKNVMISGNNIIV
                     INESEILSTISKCKDVTIDKEEIKIIRDKIFDCMKTTNQNTREHVRNLKNITEEDKCP
                     RCQIGHLIKRKGQYGEFWGCSQYPKCKYTRK"
     gene            44865..45977
                     /locus_tag="KQD82_000815"
     CDS             44865..45977
                     /locus_tag="KQD82_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4379302.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLAGFLLDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLAGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEHMLKLLEVAKGTNLELPISLAIGLGLRLSEVLGLTWDNIDFDENTI
                     TVNKITSRLDGSVILKEPKTESSVRKIFAPIELMNLLKNYRLEQNKKLLRSIVRNEYN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            46554..46931
                     /locus_tag="KQD82_000816"
     CDS             46554..46931
                     /locus_tag="KQD82_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4379303.1"
                     /translation="MYLRKLSKSELIVMKFIWNLDTKVKSYQIINYMKEKYSWSEKTT
                     LKTLSKLSNKRFIYVQETSQCTYYTISIKEDKYHEFISQKIHKLLGCNSIKSLLASLF
                     QEELTDEKITSLEEWVKNWEEEE"
     gene            complement(47072..48333)
                     /locus_tag="KQD82_000817"
                     /pseudo
     CDS             complement(47072..48333)
                     /locus_tag="KQD82_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895108.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(48488..48814)
                     /locus_tag="KQD82_000818"
     CDS             complement(48488..48814)
                     /locus_tag="KQD82_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4379304.1"
                     /translation="MSMQKIPQAELKVMKFIWNKNDIVTSKEVMEAMELEYNWKATTT
                     LTLLSRLTIKRFLDSERIKRITHYTILITKEEYKIFETKRFLEEVHSNSIESLISSLE
                     DCYKNK"
     gene            complement(48946..49299)
                     /locus_tag="KQD82_000819"
     CDS             complement(48946..49299)
                     /locus_tag="KQD82_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379305.1"
                     /translation="MLEEDEIYNVIGKRIRNYRRKAGYNQNTLAKKAGLFPAYIGQIE
                     RGESKASLRSIFKIANALEIPLEVLFENIIQNENDVETLSSEAYELIDSLTVKEQKAI
                     IRLIKEIIEYRKFEE"
BASE COUNT        21044 a         4397 c         8260 g        15655 t
ORIGIN      
        1 gtatagtttt ttcttatttt ctaattattg aatttaattt ttgtattaat tgctattaat
       61 aatttctgta ttaaactaat gagtaagtta taactatgaa acttatctta caaagttaat
      121 aaatatatat tgcattaagt aataatataa ttacattcaa agcgttatat aaatatatat
      181 tatttaatac ttcatatctt atatatgtta tcatttttat ttaccttatt tttacaaatt
      241 attccataag tatattataa ttatcttgag ggaaataatc atatagaggg agaaattaga
      301 aaatggatag atttgaagta ttaaaatatc taaaatttaa tgtttggcat tcagaatggc
      361 cattagagct tctcaaaggg gctataacaa aggataatgc tactggtaat ttattattac
      421 aattaaaaat gtgtaatata tctgataagg agataaagtc tgcatatttt acaatagaat
      481 gttttaatga tgcaggagat ttaatagata ataatttaaa gtatgtttat caagatataa
      541 acattaaacc acatgaatgt cttggtgatg atattgctat aaaaataaat gataataaaa
      601 caagagcaat aaatattata ttagaacaag tagtattttc agatgataaa gttcataaat
      661 atgaaagaga agaaggttta attataccta aattagataa aatagaattg ttaaatatag
      721 aattattaga agaacttaaa aaagattatg aagaatatga ttttcaatat gaaattaagt
      781 acatgcctaa tataatagaa aaaaaagcat ggagctgttg ttgtggaaaa ataaataaat
      841 tagaaaaaga taagtgtgat aattgtaaga gaagtaaaat actgcaaata aaaaagataa
      901 ataaaaatta tttagaatct atttataaaa aaagcctaaa aaagaaaaaa gaaatagaaa
      961 taaagcttga aaaagataat agacataaat taaagaacat aaataaaaat aatagaagaa
     1021 catcaatatt attaataagt atattattga tttttattat ttttattggt aaaaatatta
     1081 ttaataagca tgctttttta gatgaaataa agtataaaaa tgaatatagt aaaaaaatat
     1141 ctgtagaaaa agataatgta tatatgttaa aaaataatgg aaaagtaaca catagaaaag
     1201 tgaatggtga aagtagttct atacctggtt gggaaaatat aatatctata gcatcatatg
     1261 ataactcaat aataggaata aaaaaagatg gtacagttgt ttctaaagga cttggaaatt
     1321 cagattcaga caatattagt aaatggagag atataataga tatagaatgt gatagtggaa
     1381 cttttatagg attaaaaaag gatggtactg tcgttgctac tggaaataat gattatggtc
     1441 aatgcaatgt gaataattgg tttgatgtag taagtataga aataacaaaa tcaaccattg
     1501 agtatatgaa aaatacaaca gtaataggca taaaaaaaga tggaaaagta ttacttacag
     1561 gatatgatag tctgaatcat agaaaagaaa cagacaaatg gagagatata tcaaagataa
     1621 aatgtgggtc ttatggcata tttggaataa agaataatgg cacagtagtt gtatcaggta
     1681 gtgcttatga tattaagaat tggaaagata tagtggatat acatattgga ttatgtggaa
     1741 tagtgggtat taaaagagat gggacaataa taagcacaga tgaaaagttt aatgaaatca
     1801 ttaaaaattg gaaggatatt atttctgctg aaataataga taatcatgaa ggaatttcta
     1861 ttgtaggaat taattcaaat ggagatattt taacatctag tagaggtcag aatttggata
     1921 atataaaaag attagaagat gtagaatata taaattttga tgactcgatt gataattttt
     1981 tagtaggtat aagaaaagat aatgtaattt ttataagagg aataaacaat actataaatg
     2041 aagaatacaa cgaagaaata taaaaataaa tattttaatt tggaggaata gaaaatatga
     2101 aaaattacat agcaagatgt ttatcaaata tgggagtatt gttggcaggt atacaagtat
     2161 tagcgggtat aataatcatg tgtcaaaaag tagaagttcc atatgattat ttgtcagata
     2221 cttatgaaca atatagtcct attatgattg gtattggtgc tggggttata gtaagtggaa
     2281 tcgttatatg tttagtttta aagggattag cagagttagt tgaaaattca gctgaccaaa
     2341 cacaatatca aaaagaaata ttaatgttgt tgaaaaataa taatgtaaat gaaaaaatta
     2401 atgaggttaa aattgaacaa caatttacaa atgaggaatt accaaaatta taaaatgttt
     2461 taaattatat actaatactc agtactaatt aaagtactga gtatttttat gtaacagtaa
     2521 attatattaa tataaaaata tattctacat acataaaatg ataaaataac catttataat
     2581 acattaaatt acattaaatc aaaaaagtat atgtcgaaaa catataatat taaataaata
     2641 tatactttaa ggagtgattt aatgattata actatttgcg aaaatgatga tatgcaaaga
     2701 gatatcatct atgattttgt taagatattt ttaactaaaa tcaaggtaaa acattatgac
     2761 attttaatgt ttaagaatgg ggaagaactg ttagaagaat atccaaacaa agtggattta
     2821 attttaatgg atataaattt aggaggtaga aatggatttg atatttcaaa agatattaga
     2881 aaatttgata aagatgcaag aataattttt gtaactgtat cagagaaata tgctatcaaa
     2941 tgttatgaga taaatgcatt tagatatatg ataaaaccga tatattataa agaatttgaa
     3001 aaatatatgc ttgactttgc aattgattgg agaaaaagag aagctattat tactataaaa
     3061 agaaaaaaac aaataataaa aatagaggaa cataatattt tatttgttga aatagtgaat
     3121 agaaaattaa gagttgttac taaaaatgaa gaacatataa taaatgataa aatggatatg
     3181 tttgaagaaa agttaaaaga ggatatattt tttagatgcc ataagagcta tcttataaat
     3241 ttacttgagg taactaattt caattataat ttcgtttatt taacaagtga gaaaataaaa
     3301 attaaagtag ctcctaaaaa gaccaaaaaa cttaaagaga aattagcaaa tttttttagg
     3361 gaaataatat ggtaaattaa agaaaaattt ctataatcga atatatgttc ttgataaaag
     3421 tatgtgtaca agatataata ttattagtag gaaaatagga aaaatattaa tatttttgaa
     3481 cattttgaca ttaattgtga ttttagtgtt gaatttctgt taaaaaaatc ttattattta
     3541 atcatagaaa tctactgtta tagagtagaa gattgttaat tataaatgat tttaaattat
     3601 ctgagataaa attttttgtt tctgaaaaga acgaatgttc gaatgaggtg tagagtgaga
     3661 ggatgtttat taaaagatat tagattatct tacttaattc ataatgcata tatgggggtt
     3721 ctataataat gaaaaaacaa gtcacaaaag aaatacttaa aatgctagaa aaaatggatt
     3781 tatcttattt actcataatt aaaagttttg tatctaaatt aaaatagccc ttattgggct
     3841 atttactaag tttatcaatt actttctgta tgacttctct ttcatcatca gtaagttcag
     3901 caaatgttaa gaatactttt tttaaaaatt catcattact tgagaatgtt ttacccatca
     3961 ttgctgctaa ttcaatatca tctatatttt taaacatttc tccatttcca tttcgtaacc
     4021 agtcctcatt tacattaaaa acttcacata tctgaaatat aaatctatct gttagctttc
     4081 tttctccttt ttcaagtcta gaaatagttg catcacttac taataatttt tctgcaaatt
     4141 ctttttgtga gagacctaat ttaattctta attcctttaa attctcattc atatttacac
     4201 ctcctattat atagtatatt aataaaaatt gacaatgtca ataaaatagg aatgataaat
     4261 tgcaaaaagc aaaaaaaata attgacaaat acagaatgtg gtactacaat gtaaatatac
     4321 aaaacgcaat taataatttg acaaacgcaa ataaataagg aggtagatta tttgtataat
     4381 aataatgata aaaaattaat aaatttattt gaatcattta aaaaaataga ctatacagaa
     4441 caaagtttag tgtatttttt atctttaagc tttatagtaa acagaaaatg tcataaagat
     4501 gcaaatttgt atagtaggag aggtgaatgt aattatgaat aatttaaata tcaaaggaaa
     4561 acaaaaattt atggatttag aaatacctat tatagaagga ggttttggag agggtaaaaa
     4621 atgtataaca gataaaacta tatcagaaat acacaatatg gagataaaac atgttagaga
     4681 attaataaat aggaacataa gaagatttat aaatgctatt gattttatag atttaaaagt
     4741 ggtcgttgat aacgactaca atttagaagt attggagttg cttagttata gaaaaatgca
     4801 aatatcaaaa gcaaataata tttatctttt atctgaaaga ggatattcaa aattaattaa
     4861 gataatggat actgatttag cttgggagat acacgataaa ataatggatg aatacttcaa
     4921 tatgagagaa caactcatag atagttatat gatacaagat tctattgaaa gagctaaaag
     4981 atggatagaa gaagaaaaag aaagattatt acttaaagaa acaattcaag ttcaacaacc
     5041 taaagtagaa tatcatgaca aagtattaaa tcctgacaaa ttagttacga caacagatat
     5101 tgctaaagat ttaggaatga cagcacaagc attaaataaa ttactacatt taaacagaat
     5161 tatatatccg gaaagaataa atggtcgaat aaaatgttgg aaaccatata aaaattattt
     5221 atggttgata cctgaatatg cagattttaa aataacagaa catgatacgc agcttaaatg
     5281 gactgagata ggtagaaaat ggttaataga atttgtagag aacattaaaa aaataagtta
     5341 aatcaagaag ttaaaatgta ccttgaaaag tagatattta aaaatataaa agataggtga
     5401 ttaaatgata aaacaaatac taaataaaag aggtagtcta ttaaatctag caagtgagta
     5461 tttgacagaa caagactacc tcaagtttca aggatggtat acacatagat atagtaaact
     5521 ggatgcttat gatgcttcta taaatgaata tttaaattat aaaaacgaca aaaaattaat
     5581 aacttatgta tttaatttaa aatttcaaga agctctcata gaaatataat attactaaaa
     5641 atggtgattg aaatgaatta taattatgta actaaaattg acttgaaata tggaaataca
     5701 caatttggat ttgagaaata tagcgatgct ttagataagt acagagaact taaagaagaa
     5761 tttaagtatg ttccatgtat tataaaactt cattcatatg tagcacacca agatttatat
     5821 gtgaaagaaa ttaaagctga tggttttgaa aagaaatatt tagatattaa aaatttaata
     5881 aaattgttta aagaacataa acaaaatata tcagatgaac taagtctttg tgataagaaa
     5941 aaggaagaag tattacatgt aatagaagaa atgaatttag acacagcttc tttaagtgat
     6001 aagattgata ccattaaaga attaaaaaac actttagcta agagaaggtt ggttaaatat
     6061 gaactaaata aatactttgc cttttgtaat acattatatg aaattgaaga agagttaaaa
     6121 aaatatgaat ctaaatataa acaaaaagaa aatcaaggaa aaagcagatt caaaaaaaat
     6181 aaaaaatagg tgattagatg gaagaatatc aatttaactt aataaaaaag ctttgtgctg
     6241 aaaggaaaat acacaaagct tggtttagga atattgaaaa atgtggttgt ggaggtaaat
     6301 gtaataagaa aaattgtaag atgttagaaa aattataggg aggtacatat ggaaaatcaa
     6361 actgaattaa cagaaaaaga tatagagaac actttaagtg ctttaaatat tattaaatat
     6421 atttgtataa aatctaaaca ttgcaaaaat tgtcctcttg gatatatgaa aaatcttgat
     6481 gaacataatt gtatgctata tgcattaata gacagagggt tgctaccatg tgattggaat
     6541 gcaaagaaag ttacaagatt gttagatgca atagttgaat aaaattaaca aagaggagaa
     6601 gatatatgat acatgaactt aagatattac ctaaatatta tgaagctgtt tcaaatggtt
     6661 ctaaatcatt tgaaataaga tttaatgata gagattttaa aataggagac actttaaaat
     6721 tacaggaata tatagatggt gtttatacag gaaggagttt acaaagagaa gtatcttata
     6781 tacttaatga ttcaaattat attaaaaatg ggtacataat tttagggtta ataaataaca
     6841 caaatcatta tatgtttaaa gttgtaagag attataaaaa agaaaattat ttaatatgta
     6901 gacaagggaa agtgttcata gggtatattc ttgggaactt tgcttgttgc aaggagtctt
     6961 tagaacaatt tactatacca ttagaatata tagagaaaat aagtagagta gaatttaaac
     7021 cattgtaatt aaaatcaaga ttttaataga aaaggagtga ttaaaaatga agaaaaatcc
     7081 aataagaatt aaaaaatgtg actgtggtgg agatatatat gaacttaata cacaattagg
     7141 tactatgtat caatgtaaaa gctgcgggga gatgtataga tagtgctgaa atattagcta
     7201 aagaagatga ggtgaagtca atgagtaaaa aagataagtt atctattttt ataagtttaa
     7261 tattaagaca taaaccagaa actattggaa ttaaattaga tgactatgga tatgcagatg
     7321 ttaatgaatt gattgaaaaa ataaataata ctggtagaaa tattaatata gaaatccttg
     7381 agcaaatagt taaagaagat aataagcaaa ggtacagttt taatgatgat agaagcaaga
     7441 ttagagcaaa tcaaggacat tcaattaatg ttaatgtgga attgagggaa ttagaaccac
     7501 caaaatatct ttatcatgga acagctacta gatttttaga caatattaaa aatgaaggta
     7561 ttattaaaca aagtagactg tatgtacatt tatcaaggga tatagatatt gctgttaaag
     7621 ttggtaaaag acatggtacg cctgtaattt taaaaattaa tacaggcaaa atgtatgaaa
     7681 atggttataa gttttactta tctgaaaaca atgtttggtt atgtgagtat ataccattta
     7741 aatatgtaga gatttttgag tagttaaata aaattattca gtgaaataaa aaatgaatga
     7801 aggtgtaatg aaatgagcag gtgtaaagat tgtttttact ttgaaaaaaa caagatagaa
     7861 gagagtggta tatgcaataa gaaagaaaaa gaagtgtata aagagaatag aaaatgtgaa
     7921 ttttatgaaa gtaaaaaatg gaaatcaatg aatatagatg aattttggat tgaaatggaa
     7981 agatggacaa atgaaaaagt aaataggtat ataatatatt tttctgaaca tgacagtatt
     8041 cagattgata ttgtccaaga tattaataga cctaataatc ctactgatga tgaagttata
     8101 gaagaatatg ttgaaaataa ctacggttat gtaaaatatg aagttattga tttaggtact
     8161 gtagagaacg ttaagttgta aaattaggag ggaattaaat atggagaata tagaattaac
     8221 acctagagat tattttgaag atattaaaaa tagaaagcaa aaaaatacaa atgaaaagct
     8281 tgatataata tatgaaaact ctttgattct tttaaacaaa tatattgtaa ctggtcaaaa
     8341 aaagggtgct aagaaattaa tgtttttact tgaatgtatt gaaaaagaaa gaaatgtaat
     8401 agagctggga atagatacat ttgtgtataa agaagatata gaaaaatata ttgatgatat
     8461 atctaaaaat acagtaaaaa taatagaact tgaaaattat gaaagagaaa taccagatga
     8521 aatagtagaa ataatagaga aaacaaaaga tatatttgat aaaatgtatg ttttgtttac
     8581 tgattataca ggtaaaatta caaaggaaat aagcaaagag agaagagata aagacccaat
     8641 actatttggt gttttttcag atgagaaaag cagagctatt gtagatagat tctattatct
     8701 tggagattgg gaagatgagt attgtgattt aacattagag aaaatgataa atgattataa
     8761 gaaaaaagga agacaagaca tattaaaaca tataattaca cctaaatcat tagatgagtt
     8821 aaaaaaagaa ttagattctt tagaaaaaaa taatgataca tataagcaac ttgccaatat
     8881 aaagccatta cctactacaa aaaactctag tgtagaacca cctaaattaa atatatttaa
     8941 aaaaataaaa aatagagtat ttaaaaatga aaagtaatgt tgatttaaca gagaataggg
     9001 attttagagc tgtaagagga agaggcatca atattttagg tatcgaagat gcaccaaata
     9061 gaatgttgaa tggttttagt gattttggaa ttggacgtct ttatggtaaa aatttagaat
     9121 ttgaatttaa taaaaatgaa gtatttttaa ctggaaatgc gaatgataga gaggaaaaaa
     9181 agtttcttat actttgtgaa gaaaataagg tgtgtgtcag atgtggagat gaattaaaag
     9241 agagatggga taaagtaagc atagaactat gtaataaatg ttatgaaatg ttagatgttg
     9301 agtataaaaa taaaaagata attagagaat tattttttag agatagagat gtaaatatag
     9361 agttacttta agaggtatat aagaaaaata agtagaggag gaatttatat gaaagatgtt
     9421 tggttatata gttgggatga tgaatgtttt agaagtgatg aatacgaaag taaagaagaa
     9481 gctgtcgagg cagctaagga agaacttaag aggctcggag aaattaaacg atgggtttat
     9541 attggaaaaa aagaagaagc tgatattccc gacatagatg caaaagatgc tttagaatgt
     9601 attcaaaata gaattgatga cgaatacgga gaatatggag aagattggtt tgaaaatata
     9661 cgtgctgaag acatattgat actagagagt agaataagcg aagtatttaa aaaatggata
     9721 gatgacttta aatataaacc atattggttt gctgttagag atatagaaga aatagaacta
     9781 aaaaatgttg ttaattaaaa ctagtatttt caatgatgac aacagtatga caagaataat
     9841 gagtatataa aggagtgatg tttattaaag gttttagatg tatatgcaga cataagtttt
     9901 caaggataaa agattgttat ttgattacaa caaaaagtca gcagcatact tgtaagattt
     9961 gggtgtgtaa atattgtgga caaattgtag ctggttcaac tcctgtttat catcctaata
    10021 tgaaagtcaa tgagtattgt gtaaagtgtg agcttttagc atgttcaaat aattatgaag
    10081 gatattgtaa ctctaaagat aaagaaattg aatttaagtc tatttgctta gaaggtaata
    10141 aagagatttt gcagtgtaaa aattttgatt tggaataaaa tttagcttaa gtaggagaat
    10201 tttagaatat gaaaataaat gtacatgtta aaactaataa aattggttca gactgtaagc
    10261 tggagtttga aatagatgat gatgatttaa aagatatgtc tgatgaagag agagagggtt
    10321 atatagataa aattgcatgg gattatgttt ttgaaaattt tatagattgg aattggtatg
    10381 aggttgaaga ttaagttaaa acatttgttt taatgtgatt ttacctagta gatacaagga
    10441 ggtttttaat gaaagagctt ttagaagtaa aaaaaatatt tgatagttta gcatctacat
    10501 ctagtagaaa agaaaaagag agaatattag aaaaatataa aaataataga atgtttgttg
    10561 aatgtttaca atttctacta gattcaaata ttctcactgg aatatctaag aataaaatat
    10621 gtaagaattt aaataatact ggtcataatg aattagagaa catatatgat atgcttgact
    10681 atttaattaa gaataatact ggcagaaata tagatgtaaa aacaatacaa gtatttgcca
    10741 gtaaagatga aaaattaaaa gattttattt ttaatttagc tacaaaatca attaaattag
    10801 gaataacata taagactgta gacaaaataa tgccaggttt aattatctaa taaattttgt
    10861 attttttaca aaaaactatt gagaacatag aggttatatg atataataaa aatatattga
    10921 gaacagagaa gttaaaaagg ggggcaatga gatgactgac aaaataataa tagcaagtgt
    10981 agatggagag gaaatgatat ttagaactga aatgaaaaag ttagaaaaag gagatagtct
    11041 tatagtttct gtaaatggtg taaaaagatt agctctattc attaggtata taagtacttg
    11101 tgaagaatat atagaagata tagcatttca aaaagtagat gaaatattat atgaaaacac
    11161 aattgccaaa gaaaaagaaa gagttaaaaa ggtatttaaa gataagtgta tgaaaaatta
    11221 taaacctatt gttaatacag aaatagactc ttatttgaga gaatgtggtt tcaattgtga
    11281 tggtagacaa tttttcaaaa gtatagacaa tacacaaatt ttaattagca gggattgtga
    11341 taaagatgtt ttgactgtat cgaaaggttt gacaataact aaaacagata taacaaataa
    11401 aaatttaatt acagttatta aaataataaa taatagtcta ttaatagtta ggtaatagaa
    11461 ataaatattg aacttttaat aagaaaaaag attttaaata aaaggagtga tttaatgaag
    11521 aatgttatta aaagagatgg gaggatagtt ccctttgatg aaaagaaaat aataaatgca
    11581 atagaaaaaa gctttttatc attaaggaac aaaggaaaaa gtgaagtatt tgatagagaa
    11641 ttagcaacac taatagctaa aaaaataaaa aataaaccat gtgaaaaaat gcatatagaa
    11701 gatatacaag atataatcga atttagctta atggctagta aaaagaaaga tgtcgctaaa
    11761 gaatatataa catacagaga gaaaagaaat atagcaagag gaagaaaaac ttataatgat
    11821 tatatgtcta ttgtaaatac tgaaagtaat gatataacta aagaaaatgc aaatatgaac
    11881 gcagatactc cagcaggtat gatgatgaag tttgcaagtg aagctactaa aacttttact
    11941 gaccatgtac ttttatctga agaagcaaaa gaagcaatga tggataatta tatacatata
    12001 catgataaag actattatcc tacgaaatca ttgacttgta tccaacatcc tttagataaa
    12061 atttttaata atggttttag agcgggacat ggctctagta gacctactaa aagaatagag
    12121 acttcaagta tattaggatg tattagttta gagactgtac aaaatgaaat gcatggagga
    12181 caggcgatac cagcttttga tttttataat gctccgagtg taagaaaaac atttatagaa
    12241 gaaataaaaa atatagaaat aaaggatgat attgatttat ctcatttata caatatagaa
    12301 atagaggatt atataattaa ggacttaaaa gaattagaca gcgaaaaaag aaatgttcaa
    12361 catgctataa acagaactgt atggagagtt catcaatcaa tggagtcatt tatacataat
    12421 atgaacacaa ttcattcaag gggtggaaat caggtcgtgt ttagttctat aaattatggt
    12481 acagatactt cagcagaagg tagatgtata ataagagaaa tactaaaatc tacttatgaa
    12541 ggagtcggaa atggagaaac accaattttc ccaattcaaa tctggaaagt gaaagaaggt
    12601 gtgaactata atcctactga tagaaattat gacttatatg aattagcatg taaagtatca
    12661 gctaaaagat tttttcctaa ttttttaaat ttagatgcaa ccttcaataa gcatgtaaaa
    12721 tggaaaatgt gcgacccaaa cagatatatt tatgaagtag caacaatggg gtgtcgtaca
    12781 agggtatttg aagatttgca tggagaaaaa acaagtatag gaagaggaaa tctatctttt
    12841 acgactctta atttagtaaa aatggcatta gaggctaaag aaaaatatcc aaataatgaa
    12901 gctaatagag ttgcagagtt tatttttctt cttgataaat attcagatgt agtagcaaga
    12961 caattatatg atagatactt atttcaatgt actgctaaag caaaacagtt tccactatta
    13021 atgagtgggt tatggaaaaa ctcagaaaat ttaaatccaa atgatgatgt aagcgaagtt
    13081 ttaaagcatg gaacacttgg ggtaggattt ataggtctag cagaatgttt aatagctctt
    13141 ataggtgagc atcatggtga aagcgaaaag gcacaaaaat taggtttaga aatcatatca
    13201 cttctaaagt ctaaagtaga aaaatataaa aagaaatata acctcaatta ttctgtatta
    13261 ggcacacccg cagaaggtct ttctggaaaa tttactcaaa aagataaaaa aatatttgga
    13321 gaaataaaaa atattacaga caaagattat tatactaata gttctcatgt tccagtttgg
    13381 tataactgca atgttgaaca taaagcgaaa atagaagcac cttaccatga attagaaggt
    13441 ggtggccaca tattttatgt tgaattagat ggggatgcaa cacataatcc taaagctata
    13501 atgaaagttg tggatttaat gaaaaagtac aatatgggat atggttctgt aaatcataca
    13561 agaagtagat gtatgaattg tggttttgaa aacgcagatt cagagttaaa agaatgtcct
    13621 aaatgtggca gcaatagtat aaatattatc caaagaatta caggttattt agttggaact
    13681 acggatagct ggaataatgc aaaattagct gagttaaagg atagagtcac acatggataa
    13741 agactatatt tatattttag atataaaaca tgacacaatt gttgatggag aagggtttag
    13801 aacatctatt tattgttctg gatgtaatca tatgtgtaga ggctgccaca atcctcaatc
    13861 ttgggatata aaaaatggaa ctttaactaa agtattagat atttataagg aaatcatttc
    13921 aaataaattt tcagatgtaa cattttcagg aggagaccca cttctacaac tgcaaggatt
    13981 tgttcattta gctaaattaa ttaaaaagaa tacaaacaaa acaatatggt gttatacagg
    14041 ttttaagttt gaggaactca taacagacaa tgaaaagcta gaattgttaa aattaataga
    14101 tgttcttgtg gatggaaaat ttgaagaaga taaaaaatct ttagagttga tttttaaagg
    14161 ttcgtcaaat caaaggataa ttgatgttca aaaaacattg tatgaaaaac aaatagtatt
    14221 atatgaataa gagaggctta gccctctctt tggaggtaaa tgttatgacc aaagaagaat
    14281 ttattactaa atttcttaaa gagcagcaga gaagaataaa ggttttatat tatgaagttg
    14341 aaaatttcaa gatggataga aggtttaatg agaggaaaaa gtctaggaga cattaaaatg
    14401 tttcttttaa tataaggagg aagtagctat atgtattttg aaacggacat tataaaaaat
    14461 aagaatactg taaataaaat aataaaatat tgtggtggaa atactgaaaa attgaaagat
    14521 tggctaaaag aaatagaaaa taaaagtatc agtagtttag aagaattaaa agaatatcca
    14581 ttaaaaagaa attataaatt tgtattttcc aaagaagaag ctcctaattt agataagctt
    14641 ataaaaatga aactaagaga taattgttta gaaaaatgtg agatttgtag tttagaaaat
    14701 ctcttaagta tgctaaatga tgagatttta gatttaaata aagaagatat ctcttatgaa
    14761 gataatattg aatggattga aaattgtaaa gaagaactaa ttaaattaaa ggtaggaagt
    14821 atttgcatag attggtagaa aaataaagga gtgatactgt gagattttgg tgtgagaatt
    14881 gtaataaata cttcaatgta gaagaaacat tacaagaata ctattatttc ttaaatgaag
    14941 atgtaatagt atgtcctagt tgcaaaagag atttaatacc tatagctagt aaaacggaat
    15001 tatccttagg ttctgatagt gatacaaacc aattagctta tgttgaatat gattgtggtg
    15061 attatagttt acttagaaag gtaaatgcag atattgaaga tgtagtaaaa cccataatac
    15121 attacataaa aagtttaaat aaaaatagtt tagatttaaa tgggattaca gaatagtgcg
    15181 gaaagcccac tcctttaggt gtgggatgga tagcacaaac agtgtataaa aagtattgac
    15241 acatacacgt ataatgttaa tactttgtat tctgccctag atataaaaga aaaatattta
    15301 atatagaagg tttagaagat agatttaaaa cattagttaa aaataagtgt aaggaaatgg
    15361 atatcgaaat tgttgcaata gaatgcgata aagaccatac acatatgttt ttaaatagtc
    15421 ttccgactct aaatccttct gatataatgc aaaatataaa aggctataca agtaaaatat
    15481 taagagaaga atttattgaa ttatcaaaaa tgcctagttt atggactaga agctattttg
    15541 tatctacagc aggaaacgta tgtagtgaaa ctattaaaaa atatgtgaaa aaccagaaaa
    15601 caagatacta gaaagtgagg tgaacactat gtcaaattat atattaacat tgcaattaaa
    15661 aacagaaaag tttcaagaag acattatatg taatgctttt gataagtata gaaaagtata
    15721 taattcatgt ttatcagagc tatttaaaag atataaccac atgatagaat ctaaagaaca
    15781 ccaagcaaat tgtaaatata agggtaaaga tagaaataaa atatttagtg aaattaataa
    15841 gaagtataat ttaacagagt attcacttca taagtttgtt aaatttattg gtaaatatta
    15901 taaactacat tctgctataa ctcagaagat agctacaaga tgttttaatg cttttaataa
    15961 gtatatctat catcaagcta aacgagttaa ttatattaga tataatgaat taacatcaat
    16021 agaagggaaa caaaattcaa ctggtattag ttataaagat ggtattatta aatttaataa
    16081 gatgactatt ccagtaataa taaagaataa tgacaattat gctcaaagag cgatacaaga
    16141 taaaataaaa tattgtagaa tacttaaaaa agaaattaag ggtaaaatta aatggtatgt
    16201 acaattagtc ttagaaggaa ttccacctaa aaaaacaacc aatcaaggaa aaataaaagg
    16261 acaaattggt ttaggtaatg taggtataga tattggaact caaactatag ctatatctag
    16321 taaatgtgat gttaagttat tggagttagc tcctaatgtt aataatatag ataggcaaat
    16381 aaagttaatt caacgtaaaa tggatagaag taaacgtgca actaatctta ataaattcaa
    16441 tgataatggt actataaaac aaggaaatag agataagtgg atatttagta atcactatct
    16501 taaattaaaa agactaagaa aagagttata tagaaaacaa gctgaaataa gaaaacaaga
    16561 ccactatata ttaattaaca aattactaaa tttaggcaat aagttttatg ttgaaacgat
    16621 gagttataaa agacttcaaa ctagagtcaa ggatactact attaatgaaa aaactggtag
    16681 aattaataaa aagaaacgtt ttggaaaatc attagcaaat aaagcaccat caatgttttt
    16741 gactatgtta gacaataagt taaaatataa taatgaaaga ttatataaga tagatactgc
    16801 taaatgtaga gccagtcaat ataatcactt ttcagatgag tacaataaga aggaattaaa
    16861 agatagatgg aataatggta tggacattca aagagattgc tattctgcat ttttaataat
    16921 gaatgttaat gaagatttaa aatcaatcaa cagagaattg tgtgttaaaa catatgataa
    16981 ctttaaaata ttacatgata aagagataaa tagattaaaa aaattaaaat taaatggtta
    17041 taaattaata tctagtatgg ggatataaaa actatatagg ttgagaaacg agcctaatat
    17101 taatgttaat ggtgtcaaca gatactttgt taataaaagt cttagggaat atgattagtg
    17161 ataatatgtt gtaggttatg atttattata actgagagta ttgtagaaat tcatgtagta
    17221 cctaagaacc ccacgccttt aggcgtagga gtttcagaat aacaatgaat ggtaatatat
    17281 aagaataaaa agaaggatat tataagagat gagtcgatgt atgattatgg gatgtctttg
    17341 tcatctatta tgaaggttat cttaaatcat caagaagata tagaaaagtt aaaagaagag
    17401 gaggtagcag aatttataaa aaataaatta gaaaatgagc atgattgtga tgaattaaaa
    17461 ataataagtg aggtatttaa tgaaatatat taaacatgta gataatggag taatgagagc
    17521 aataggtatt gataattata aacctttatt aaacatcata aaagaaaaag tgaatttaga
    17581 atctaattaa aacagtttta attagcgagg tgatacatat gggattagat ttaggttttt
    17641 attcagtaaa aacaaaagtt acagatttag atatttattt aaataaaaca agaggtgaga
    17701 aatataagga attgaataat gatgaagaag agttttataa gtttgaagaa ttattagata
    17761 ttgatatatc tttagaagat tggtcagtta ttcgtatgat atataaagcc aaatgggaaa
    17821 aaggttatga aagttatgaa gatgaagcca tacttattac aaaggaagat ttggaagatt
    17881 atataattcc ttttcttaaa caaaaagata taaattctaa gatatacaat aaggataaag
    17941 agtattatta taaaaatgat aaaaaagtaa cagttgatga taggtataca caggagtttt
    18001 gggatttagt attaaaagaa tttcaaaaaa tgttaaacat aattgatttt gaaaaacata
    18061 gtttaattat tatgtattgg tattaagata ataattttaa tttatttgga ggtttataat
    18121 aatgaaaaaa atgtacatag caattaattc agacttaaat atgagtccag gcaaagttgg
    18181 agcacaagtt gcacatgcag tttatgatta tttgtataac aaaattttag acactgtttc
    18241 ttgctcttat gaaacagaaa taatagcaca tgaattaata gatttaaaat gtgatttaac
    18301 gtcatttaag aataatgggg atacaatatg tatattaaaa gctaaagagg cacaattatt
    18361 aaaatttaaa gaaaaaggtt atttaactat agtggataga ggattaacag aaattcctaa
    18421 aaatagcata acatgtgtta acttaggaat atttaatgag gatgaaatac catctacaat
    18481 taaaagattg agattatatt aaggagaaag acaataaatg tattctaaag gtgatgtagt
    18541 aaaagtatgg tgttatcaat gtgaaaggat agaatttcaa gaatttgtag gatattttaa
    18601 ttcttatttt gataattata aattaaagaa acgatggaaa tgctctaagt gtgatgtcat
    18661 caataatttt taagagttta attaatggag gaatgagtta tgaaaataaa gaaaataatt
    18721 ttaggattag ataacttaga gaatgtagag ttgggtattg gagatgtagg aggtttttat
    18781 ttagataata tacatgaaat tattggagga tttgctttga gttatatagg taaatgcaag
    18841 aaggcagatt atttacatat acaaatacat aaacgtgttg atgaaattca aagattgcat
    18901 gattataagg atattacaaa tgtaaagata gaatacgaaa acggaagtgt ggaggatata
    18961 tatgtacctt ataaacaaga aggggatttc ttgggtttag ataatcttta tcaaacttct
    19021 tttattagta aacgaggtta tttacatata gttatctcta aggataaaac agtagaagaa
    19081 gtcttcaatg aagaatttga ggatgatgaa ggggtagaat ttagttttaa tatgtatgga
    19141 atataaaact taaaatggaa ggagatgaac aaatataatt aactctttta agaaattaat
    19201 aaagtcaaaa tatataaaaa ataacgaatc aataaaacca ttagaaagaa tttttgaata
    19261 tgaaataaga atcaaagaaa aaagaacata ttttaattta gaattattga tttatgaaaa
    19321 aagaattaag ataataaaaa aaatattttt aaaagatata ctatgccatt atcgaagaga
    19381 tatagaacaa ctcgtcattt atgacctgga taaatatctt aatatgacag ataaagaaat
    19441 ggaggaatat attataaatt taataaaaga taaaataaaa gaagagtatg gggagaatag
    19501 acttcctgaa atactaatgg aaaaatttat aaaactcgaa agaaaaggtt cggtgactgt
    19561 agacgtacag gatttaatag agggataata aataacaagt taaaatacag gttttaaaag
    19621 gagaataaca aatggaagtt caagggaaat ataacaaagc taaaatattt acagagaatg
    19681 tagattcgat aacaatagga caaataataa atttatgtaa tcaaaaggaa tttaaagaat
    19741 ccagtataag aattatgcct gattgtcatg ctggtaaagg atgtacaata ggaacaacaa
    19801 tgactataag tgataaagta gtaccaaatc tggtaggggt ggatataggt tgtggaatgg
    19861 ctactattcc attagaaaaa tatattaata atataaatta tacaaaatta gataagatta
    19921 taagagatta tattcctcat ggatttaaaa tacataataa gtcaagagaa aaactattaa
    19981 agaatttatt taatatagac ataaataatc ttagatgcga ggtgaataaa aatagagcat
    20041 atatgtcgtt aggaacttta ggtggaggca atcactttat agaagttgat aaaagtgaaa
    20101 aaggtcaaat gtatcttact gttcatacag gttcaagaaa tttaggaaaa caaatagctg
    20161 attattacca agacaaagct ataaaatatt gtattgaaaa atataaaaag tcatatgatg
    20221 atgcaaaaga gtttttaata tctactctaa aaaaagacaa taaagaacat ttgataaata
    20281 aatatttgaa agaccttagt gatagaaaat tgaataagcc acatgatgat ttatgttatt
    20341 tagaaaatga tttaatggaa gattatttac atgatatgag tatagctcaa aaatacgctg
    20401 ctgcaaatag aatggttata attgcggata tattgtttaa atataacagc ctagatgtaa
    20461 attatgacca ttttataaga gatattaaat gtgatgttat agaatgtatt cataactata
    20521 tagatatgga tagtaaaata cttagaaaag gagcaatttc tgcaaataaa gatgaaacag
    20581 taataattcc agtaaacatg agagatggga ttatattagg aaaaggtaaa ggtaattctg
    20641 agtggaatta ttcagcacca catggagcag gaagaatttt atcaagagga aaagctaaag
    20701 aaaaaatatc tttagatgaa tttgaagaat ctatgaaaga aatatttaca acttgtgtag
    20761 gtcaatcaac actagatgaa gcaccacaag catacaaacc tatagaggac atattaaaca
    20821 atataggaga tactattgag ataatggagg tgttaaaacc agtttataat tttaaaagta
    20881 attaaaatga aaaatttaat agatgaattt aggaggaaat actaatggga atgtatacag
    20941 aatttgtatg tgcgattgaa ttaaaaaagg atacgccaaa agaagttatt aatatattaa
    21001 ataacatgat agaaggagaa gatagatatg atattacacc acctcaccca ttctttgaat
    21061 gtcgtagatg gagatggtta tttataatgg atagttattc tttctcaggt aggagcaata
    21121 cgatgtttga gtatgacgat atagcaaaaa catattattt aactattaga agtaacttaa
    21181 aaaattatga tgatgaaata aataaatttt taaattggat aaaaccatat atacaaatac
    21241 attgtgaaga cgatgaattt ttaggatata gtcgatatga agaatgtcgt aatccagaat
    21301 taatttatta taatgaaata caataaaatc ttaattttaa atggaggtac atatgaaatt
    21361 tgaagataac atgattaaat cttttagaga ggttagagtt ggagacatta tacttgcagg
    21421 taataacaag tatttaataa tatcaatatt aaattatttt ggatatgtga acttagatac
    21481 tggtgattgt agttctggct ttagatcaat tgaagaatta atgaaacaat tcgataaaca
    21541 aacacgattt attcaaaatg atagatttaa attaatacta gattaatata acataaaatt
    21601 ttatttttat aagaaaggta aattgaatat gtgtaagttt tgtgaaagta taattgataa
    21661 tacaaaagat atacttttaa cctcaagaag tagattatta gaagacaata catgtgaaat
    21721 tatagcagaa gataattgta ataattgtta tgatggatgt tatgaatatt tcaaactaaa
    21781 cggatataaa atcaggggaa atacatatgt taatgtcgac tactacaaaa aaattagaaa
    21841 aataataatt gctccatgtt cagaatcagt acatataaac tattgtccat attgtggtaa
    21901 acaagtttct aaagatataa aagattttaa ttgtattcct gagcatatag tagatgttag
    21961 atataaaaat ggtgatgtat atgattatga tatggaaaag tctgtacaag aagtcatgga
    22021 taatgcaaat aataattaaa taaaatgata cttttaatag gggataaata tgaatctttg
    22081 gtctaaaatt gaagaaccag tgatgagagc agtaagtgga atagtattta atatatgggg
    22141 aaatgtttgt ttttccatat attacataga agaaaataaa caaatagagt tagaaatata
    22201 tgatagacct tatagatatg aaggtaaaaa aaatagttgt tgagaagtta tttgattaca
    22261 agttaataaa tgcagattat aaagaaatac atgatattgt ttctatgata gaaagatttt
    22321 tactagaaaa ttattaaaag gagatattta aaatgaataa aggaattaaa aactatataa
    22381 cagcagtgaa ttacttactt gataatttag agtatatgat ggaggatgac gaatttaata
    22441 atgaaaagtt acagaaaaga attgatgtaa ttagaaaatt tgaaagagaa ttataagatg
    22501 ggaaaaataa aaaatggaga aatagatact gaagattata ggctttttaa aaacattcaa
    22561 aaagaaaaag atagagtata tagtaagtac attaatgaag aatacacaat ggaagaattc
    22621 ataaactact taagattgga agataaagat tatactacag aaatattaga aagatatgca
    22681 cgatatattg atgaaaattt agacaatcgt tgtccatatt gtgtatataa tatttgtagt
    22741 gaacatgata attgttttga tggaatactt gaaaatttaa aagaaaaaat taaaataaaa
    22801 taaatatttt aacaggagaa aaataattat gaaattaata aatggggata gatgtagtgg
    22861 aaaaactact acattaataa agtatgctta tgaaaataat gcacttatat tatgttattc
    22921 gtgtggaaat ttgaactata taattgaaaa agcaaaggaa ttgaaactag atataattat
    22981 accaagagta tttaaaactt atatagagtc taatcataaa atagaatata tgaaaactat
    23041 aaatggaaat attatcaacg atacttctaa tataagatta gttatagatg aaatagattg
    23101 ttgtttggaa tctattatta gagaaggtat agattgcgtc actggaacaa ttcaaataga
    23161 aaatttaaat aatcatatct gataaatttg tttattacat atcaaaaaat tctaagtgtt
    23221 cttatttaca attcaattaa aactaaagga gtgaaattat attgaaaaaa attttattaa
    23281 taggtgcatt aggaacaatg ttaataggaa tgtctggatg tacaggaata caaagctcaa
    23341 tgaaagatgc tgaaagtgat gtatcaggtc ttaatagagt tgtaaatgta tattcagata
    23401 atggcgaggt attaaaaaca tatacaagta aaagtatgag agtcaaagat ggagatggtg
    23461 gcactataac actagatttt gatggtaaga gggtgattat atgtaatgct catgtagtaa
    23521 tagaagaaaa tcaatctaac aaataaagga gaactaaatg tttaaagtct taaaaacaat
    23581 tcttataata atttgggtag tagatatttt aaacttccca caatttcaat tcttagatac
    23641 aacatatcca atcaatacct tagcatggtt gctaatctgg attcttatac catcaagtag
    23701 tatatatatt gataaaaaag aataaagaag ggagtattag tttgatttta aatattggtg
    23761 caatttttaa tttatggtgt atgttagcta attatttatg gggaagtact agtggaatga
    23821 tacttaatgg attttgtctt atagcattac tattattgga taataactta gattaaaagt
    23881 attattttaa ggagattaaa agatgaaaat aacaatatca attgaagaag caatggatat
    23941 tattaaacag cactatgaga ataaaggaat tgaaatcaaa gacttaagtt ttaatttaga
    24001 aaaaactgga atggttagaa atattaaatt taagggagaa ataatactta agtaggagat
    24061 atttatagtt gcatcagtaa ataaaatatt agacaaatag aggtgatact tataagtagt
    24121 acaaatagaa gtaatgctag ggattttcat gtaagtgatt attatagaac tccaattact
    24181 aaaatcaaag agtttttaac tgagttcagt agatatgaaa atatactcaa tccaaatata
    24241 aaaatattag acccatgtgc aggtggagat aatacaaatc caatgagtta tccaactgca
    24301 attcaagaat ttttaaatca agatatttca atagatacag tagatataag agatgattca
    24361 agggcaaata taaagcaaga ttatttaaag tttcagccca aagaaaaata cgatgtaatt
    24421 attactaatc ctccctttaa tttaagcttg gatataatta ataaagcttt aaatgatgta
    24481 agagaaggtg gtttcgttat tatgctgcta agattaaatt atcttggagg aaaagtgaga
    24541 caaaggctgt ggggaaataa tatgcctaaa tacatatttg ttcacaatag aagaatgagt
    24601 tttacagatg atggaaagac agatagcata gaatatgctc attttgtatg gcagaaggga
    24661 tataatccaa aattttctca gttaaaagta ttaataagtc aatgaaatag aggtgtaaag
    24721 tgaaagttaa atacgttgta tttgaatggg agattacatc aaaaaatgat ggccaaaaac
    24781 attttataaa ttttagagat ttaattaaat tatatggagt gtcgtcaaga gaatgtataa
    24841 gagcaaagga ttattacgag agagatggtt tagaccttaa agatataaaa tttctatatc
    24901 caagggatga tggaaaatat aaactataaa atcatggttt taaatgatta taaaatgtca
    24961 gttttaaagg gggctaatca tatatggata ttaagataga tatgtgttta ttagagatgg
    25021 aaataaatag aagaagatgt gagtatgctg ccagaacagg aagatatcct aattctctta
    25081 ttttgtggga aggttactat agccttctta atatccaata tatagaggat aaaacaagag
    25141 gatatattac taaatttaat ggcatggatg ttataaaatg taatgatttt ttaaaaatag
    25201 aattatttga aaagaatatt aataattaaa atttgttttt taagttgagg tgatgagttg
    25261 agatataata attatcatag tcataaaatt tattcaaata taaggtcgtt agatgtaatt
    25321 actaaaccac aacaatatat agatagagca ataaaactag gtcaaaatac atattttacg
    25381 acagaacatg gatatcaagg aaatgtatat gaggcaaaaa ctttatgtga tgaatataaa
    25441 ttaaaaatga ttattggagc agaattttac tatgtaaatg atataaatga gaaagataga
    25501 ggaaattatc atttaatagt aatcgctaaa aataatgatg gttacaagca aataaataaa
    25561 gctttaagtt tagctaataa aaatggatat tactataaac caagaataga tgaaaaaata
    25621 ttatttgaga tatttaaacc aggggatgta gttattacta ctgcatgtgt agctggaata
    25681 ttaaaacttg agaatagaga agaattaata ctaaaactaa agaattattt taaaaataat
    25741 ttctttcttg aagtacaatc tcatcctcat aaaactcaag ctttgcacaa taaggatgtt
    25801 ttagaattaa gcaacaaata taatatagat ataatacacg ccaatgacag tcattatatt
    25861 tatccagaag aatcaaaata tagaactaaa tttcttaaag ctaaaggtat aaattatcct
    25921 gaagaagacg gttttatttt agattatcca aattcagagg atatatttaa aagatatgaa
    25981 aaacaaaatg tattaacaaa gcaagaagtt gaaagagcat taaaaaatac tctaatattt
    26041 gatgagtgtg aagaaatcac cttaataaat gatgatataa aattaccatc aatatctaaa
    26101 aatccaaata aagaactgaa agaaatatta aataaagaat ggttagagaa aagaaaaaat
    26161 atacctaaaa atagatggaa tgaatactta gatgctataa ggtatgaatt tgatattata
    26221 gagaaaactc atatggagga ttactttatt atagattata aaatagttca aagagcaaaa
    26281 caagaatata atggtcttct tactaaaaca gggagaggtt cagcaccttc atttataata
    26341 actaatcttt tagggcttac tgaaatagat agattaaatg ctccggtacc attattccct
    26401 actagattta tgagtgttga aagaatacta tcagcaaaat ctttgccaga tatagattta
    26461 aatgctgaag atgctgaacc atttatacaa gcaactagag atttattagg agaagaaaat
    26521 tgtgcatgga tgataagtta taagccatta caagatgctt cagcttttag gctatggtgt
    26581 aaagcaaatg atatgaaact atctgagtat gatgaagtag ctaaaaactt agataaatac
    26641 acaaatgatg ttttttggaa agatgtaata aaggaatcaa aggtatttgt aggagtagta
    26701 gaaagtgttt ctttctcacc atgctctatg cttatatatg atagaccaat tgatgaagaa
    26761 gtaggattat taaaaactaa ggatggggta tgttgtaata tagatggata ttattgtgat
    26821 aagtataaat atttaaaaaa tgattattta acagtgaaag tatggtcatt aataagaaaa
    26881 atttgtgaat tagcaaatat tagtattcct acaattgaag aactaaacat cttattagat
    26941 gctaaaacct atgacatata taaggacaaa ttaacttgca ctataaatca ggtagattca
    27001 gactatgcta caaacctggc atctaaatac aaaatatcta gtgtggcgga aacaagtgca
    27061 tttgtagcta gtatcagacc aggatttgca tctttattag acaattttat taacagagag
    27121 tcatatacaa ctaacgttaa agaactagat atattattag aagatagtta tcattatctt
    27181 atgtatcaag aatcaataat gaaatattta atatggttag gaatagagga atctgagagt
    27241 tatgatatta taaataagat taaaaaaaag agatttaagg aaaaagagtt gaaagaattg
    27301 catatcaaac taaaaaataa ctggattaaa aaagtcggaa ctgaaaataa ttttgaagat
    27361 acatggcaag ttgtaaatga tgctgcctca tattccttta atgcttcaca tagtctttca
    27421 tatgcatatg acagcttata ctgtgcatac cttaagtcac attatccatt ggaatattat
    27481 actgtagcat tcaatttata taatgaagat acagagagaa caagaaaatt aacagatgaa
    27541 atagagtatt ttagtattaa attagaaaat cctaaattta gattttcaaa atctgaatac
    27601 ttctttaata gagacacaaa tagtatttac aagggcattg aatctattaa atttttaaat
    27661 tctgatatag gagagtatct atattctctt aaagataata aatatgattc atttttagag
    27721 ctattaatag atttgcaggg gcatataaac tctaaacaat tatctatact gattaagcta
    27781 gatttctttg aggaatttgg aaagtccaac aaacttttag agacttatga tatctataac
    27841 tcaatctata gtaaaaaaca atttaaaaga gataatttac catgcaatat aaataccatg
    27901 agaaaatact ctaataaaga aacagaaaaa atatttaaag aagttgatac aaaagaatta
    27961 tgttcttatc ttgaaagtca aattgaaaat acagatatac caaccaatga aaaaatacaa
    28021 gctcattttg agtttgtggg ttcatgtaac ataaaagata gcaattcaaa tccaagatat
    28081 tgtttagtaa tagatataga tactaaatat tctcctaaag tcacattgta taatataagt
    28141 tcagggaata taaagatttt caaagtaagt aaacctattt ttgaggataa taaaattgat
    28201 gtttatgatt tagtttattt aaaatcaatt aaagaaaaag ctaaaagtaa aaaagtagat
    28261 ggaaaatgga ttaggagcaa tacagaaaca gaatggtgga tacaagaata ttggaaaata
    28321 gaaaattaag aatatgacct cgtatatgcg tttctaagac tttacataag agtctgcttg
    28381 tgtttataca ttgatttctt aaaatggcat atacgaagtt cgtgaaaggt agtgatttca
    28441 atacttatag atgaacaaag atattatagc ttattggaag aaaaaaaaga cctaagtatg
    28501 agaatcgatg aattaaaaga ggaaaatgct tttttaaaac aagaaaatga agagtatgaa
    28561 gaaattctta atgagtggga atgtgtaaga aaagaaattg tagcaacttg taaaaaaaaa
    28621 gaaatattag gtgaaaaacc aagtgaatta gaaatggttc ttcaaaaaat agaggatata
    28681 taatgagtgt aaataaaatt tatgagaaaa aagaatacat tattatcaaa tcaaaatctg
    28741 gatatatagt ttataataca aataaagagt ttaaagaagg ccacactcac ttaaaaagtt
    28801 ataatgctgc taaaacagct atagatttag ttattaagag aaggcttcct aaaagttatt
    28861 ctatttattt tcttacaagt ttgatgagaa tatctaatga tgtacaatat ataaaacata
    28921 taagcgatat tgtatatata agaaataata aaggactaaa agaaaaatat ataaattcta
    28981 aaaatattaa aaaaaactat tgagaacata gaaggtatat gatatactat gaatataaca
    29041 agaacataga aggtaaaaag gaggtagtga ttaatatttg aaatcttata aagaaaaaca
    29101 gtttttagta tttgaatttg aaaatgggaa gaatgtaaag tataatttgg caacaggaga
    29161 atacgttggt aaatctggaa ggattgtaaa agatgtgtgt actcaactta gaggatataa
    29221 tttacatgag gtaataaatt cttttgaaga tgaaaattat aagtgttttt taaattttgt
    29281 agatgaaaga gtaaatagaa gtaagagtag cagagcttgg ggaaggaggg ttgaaagaat
    29341 aagaaatata ggaagttttt taaatgaaat aaataattat tctgagtatg aacaattatt
    29401 ctcagcagga ttaaaaaaaa taatttatcc gatacattgt gatattaaga gtatacctaa
    29461 aggtcttatt tctatctgta ggaattatag tattactttg agtgaccgat tgatacaagg
    29521 gtatagtaaa aacccaaatt tgtataataa tttattaagt ttagaattga attcaataag
    29581 aaaaactaat ttaatttcaa ttcttgattc tggttatgat agaatgatat ttgatagatt
    29641 aatagacata tataaatata aacctcaatc acttataaat tatatagata atctgatgac
    29701 atatgaagca ttaaatgggc ttgaaagcac tctaaacgaa ttatatgact atgttgttat
    29761 gatgagtgta ataagtgata aatatgaaaa atatccaaga catttcttaa caactcataa
    29821 aatagcatct cgaaattata atcgtcttaa aactcaattt gaagaagaag tatttaataa
    29881 aaggatagac aagtcactag aatattcata tcaagattat aaatttatat accctaaaaa
    29941 tacaaatgaa attaaagatg aagcagtaca acaaaataat tgtgtaagta gttatattca
    30001 aaatgttata gatggaaaat gtcatatatt atttatgaga aaaaaggata atccaagtaa
    30061 aagtttagta acaattgaag ttagaaatgg caaaatagtc caagctaaag gtaaatataa
    30121 tagagatgtc aatgaaagag agcaagaagc aattgataaa tataataata gaatagaaag
    30181 gatgaagaaa atatgttaat agaaagaact aaaataagat taatgaaaga aatacaaggt
    30241 tttgagatgt taaaaatagg agatatattt gttattacat ctgtgggaaa taatggagct
    30301 atacatttta aaacagatta tggaataggt tttatgaact atagtgaatt tgaagcatat
    30361 tttgaaattg ttcaacaaaa gaaaaaatat gaatggggtg catggtctat aagaggagat
    30421 ttctctggag catatctata tagaacaaat gggaaaaaag tagaagttag aaaaggaaat
    30481 tttaaagcat cagctacttg ccatgattca gatgagtttg aattaaataa aggtgttgag
    30541 ttatgtctgg caagaattga agttaaaaaa gcaaataaac aggtgaattt agtattagat
    30601 aaaataaata ataaagaaat ttaggaggtt agaatgattt gtaaatttaa agaaagatgt
    30661 gaaaataaaa atagagattg cgattattgt tgttataatc caaatgcata tttagaagat
    30721 gcttttgaat ggaatggaga agggagaaag ccaacagacg aagagttaga tgatgctctt
    30781 gaagaataaa taagtattta aaattatcat tttaatatga ttggaggtct ttatgaggtg
    30841 gagaaatata ttagtaacaa gtgttttagt aatgggtagt agcatacata tatttgctaa
    30901 tactaaagaa atgagtacta gcaggatgga taaaaatatt gtagcagata cagctaaaaa
    30961 tattaactta atgtataaat atgagtggga gcaacacaaa aatagttctg aatataaatt
    31021 agaaatcaag agaattaatt tagaaaagga cttaggaata aaaatagaaa aattgattcc
    31081 tgtaacatgg gaagtaagtt actatacttc tttaaactgt gaaaatagta aatatggagc
    31141 tattacagct acaggagaaa aattacaata tgggtttgtg gccaataatt atcttaaatt
    31201 cggaactaaa atattagtcg atggaaattt aaaggtggtt aaagatagag gttctaataa
    31261 atattttggt aattctaatg ctatagatgt atttgtacca aaattagagg gggagagtga
    31321 ttataaatat tataagagag taaacaacat gggaagacat tataaagaag gatatattat
    31381 agttgaaggt taggagttga taaattgaat gtaaatggaa taaaaatacc aggaattatt
    31441 gatgtgtcaa atgtgtatgt ttatggaatt aaagaaagca taattgcaag tggttatccg
    31501 atgtatgaac aacaggtaat agatatgaaa aaaataaaca tttcagaaaa agatttaaaa
    31561 cgagcaaatc aattaggcaa atcaaaacaa ggttcaggtc atgattgctt ttctaaagga
    31621 attatcattc aaatggattt acaatggtca gaatatatgt ggagacaatg ggacagatat
    31681 catcataatg attatgtgtc tagtcaaagt aaaatgcata gattatctaa atttaatctt
    31741 gatgatatgt gtaataaata tgttgataaa aatacaataa attttctaaa tgaattaatt
    31801 catgagtaca ataactttga agaattgaaa ttgaataatt ctaataatat tattcttaga
    31861 aatggagaaa aagttgaagt cacaaaagaa actctttgga aaatcataat atcaaattgc
    31921 ccatcaggat tgatgctaac tgcaagagtt acattaaatt atctacaaat aaaatctatg
    31981 ataaatcaaa gagaaaatca taaaatggag gaatggagaa ttctgtgtga cttttttaaa
    32041 acactaccaa aatataaaga attaatgatg taatagtttt gttagcaatt aaattttaaa
    32101 tataaaggag aagtgggtat ggcagaaaaa agaataaatt ataatcaagt atttgtatca
    32161 ggagaagttt tagacatttt aaatatagct agaatgaatc aaggaactgg ccaagaagct
    32221 ataagattta ccttaaaggt tgaaacagca ccaaacgaat cagtaaatgt agattacttt
    32281 acatcaatgt ttaagtcaga tggttcacca aatcaaatgt ttttaggaat agagacaata
    32341 gctaatgaaa taaaaactag agcagaagat ggaaaaggtg atattgttag atgtgtttgt
    32401 tcattagaca ataacttata ttataaagat ggtgaaaaga aagagcgatt ccaaatatca
    32461 ggaacttttt gcaatagaga aaaatatgat gacaaaggta atcctattga tgacaaaggc
    32521 gaagtagtaa agtcaataaa agcgagtcag gtctggagag tatatacatt aattgaaaat
    32581 atagaagaaa aagaagatga tttaggtaag tatttagaga ttaagggttt aattaataaa
    32641 tatggtaagc aaggttgtaa tatgacattt agaatacata atgaagatat gatagaagga
    32701 tttaaaagtt tatataaaat aggagatgtt ggccttcttg aaggtacagt aaaatctatg
    32761 gttgttagta aatcagcagg atttggttca agaattaaaa aaagtgcttt tacattttta
    32821 gaaatagaag gtggcgatga acctttgaaa gatggagaca aaatatttag tgataaaaat
    32881 tatcctttca cagataagaa tatagaggca atgagagaaa aaataaaaga aaaagataaa
    32941 aaagaaaaac aaagagatat tgataaaaat ggtaagacaa ttgagataag agatgatgat
    33001 gtaccatttt aaattataaa ttaaataatc tattaaaaag gagagatgtg aatggaagtt
    33061 aaagaaaaaa caatgaatga tggtttcaag aaagtggtaa atgaggaaat aaagacgtat
    33121 atatcaaatt tatattcaga tagaatagac ggattaaaag agagtgatag tcaaattgag
    33181 ataacaaaag cattaatagc ctttcattta gatatagaat caattccaaa ggataataca
    33241 aacccatttt tcaggtctaa atatgcaaac cttgatacaa ttcttaaagc agtaaatcca
    33301 ttattagcaa aacatgattt aatactaatg caatcagcag aagatgcagg taatgataaa
    33361 gtatatataa aaactaaact aaagcattca tcaggcgaat acatagagtc aaattctgca
    33421 ccatttaaac cagctaaaac taatgatata caagctagag gagcattgga aacttatttg
    33481 agaagatatg cagttcaatc agtattagca ttatcattcg aggaagacga tgatggaaat
    33541 tctttaacaa acaaatcaaa gggtaaatca gaagaagaaa attcaactat taagaaaagt
    33601 agagtgtaaa taaaattgct acctcttaaa tgaggtagca ataaatacat aacataagga
    33661 ggggaaatct tgaattgtaa gtgtaggttt tgtaaaaaga aattaaatac aaatgatgct
    33721 tataaagtag agcatattac atctggggga aataagcaga atagatatta ttgcaatgaa
    33781 caggaatata gaaaagagca acaggatata tatttctgga aacaatgtca acttggaata
    33841 gattatatta tgggatatac agttataaat aatcaaaaaa ataagatgtt acaagagata
    33901 ataaaaggtg gttatacgag agaagaatta tatgattgca tcattgagaa gaaagatgaa
    33961 gttatagaat tacttaacta tagaaaagat atagaagaag aatatcctaa attatgttat
    34021 gtgtttacaa tattgaaagg aagtattaga gatataacaa ttaaaaacaa gcaaatgaaa
    34081 tatgaaaaag aaaatgaaag gatatacaaa gaagtagaga aaagtgaaga atattatgaa
    34141 ataattgctc ctaaaaaagt aatttataat aaaagacaaa gtttatttga caaagtgaaa
    34201 ggagtgtatc aagacgagta gttatgaaaa aataatagag gataggggta ttatagaaac
    34261 attggtttta ggcacaatgt taaaatcatt aactcttttt agtgaatata aaattagcga
    34321 aagtgacttt ataatagata aggtaaaatt ctttttctcg ttaggaagaa taatgtctaa
    34381 aacacataac gagcttgatg aaataagtgt agctaaattt gtatcttcaa ataaactcaa
    34441 atctgaatat gaaagatatg gtggttggaa tagtttatca tctgctatgg agtatggaaa
    34501 agaaactaat atagcagcat atattgatga tttagctaag aataatttac taattgcttt
    34561 agataaaaga ggttttaatg taataaaaga aatggagcat aatgggttaa aatttattcc
    34621 atttgaaata tttcaatcta tgaaatgtag agaagtggaa gaattttatg aggggttaat
    34681 atcttcatgt agtgtaaatt ctataaaaaa taatatgaaa gttgaaaatc ttttacttac
    34741 taaagatgat agagaaaaat taaagcaaaa aactgaagca ggaacacctt ataatattat
    34801 atttgaatac actgaaaaag aaataggatt gagtaataat gaagaatcta agtatatata
    34861 tagcttacca atattatcaa ataggactaa tgggattggt aagggtggtg gtataaatat
    34921 aatagcaggg ttttcaggca taggaaaaac tactttacta ttttttaatt acatattagc
    34981 tatgatatat agaggagaga agatagtcat atttgcgaat gaacaaaaat ctcaatattt
    35041 taagagtatg ttagtatcgt ttatagcata taacatattt aattatcatg atttagatag
    35101 aaataaaata gataatggag attttacaga ctttgaagaa gacttaatgg aaaaaataga
    35161 aaaattttta agagatagat gttttgcaga gaatctaaaa tttatctata tggaagaatt
    35221 tgaaatatct gaaatactaa ggaaaagtaa agaacttgtt acacatcagg gatttactgg
    35281 aatagcagtg gatacattta aatcagaaga ttcgtcagat gcacattata caggaaaatt
    35341 aattgaaaat tctaaattac tcgatagttt tgggaacaaa tataatgtaa taactatgtt
    35401 atctatgcaa ttacttacag ctcaagaaaa taaaagctca tatctttcgg caggagattt
    35461 gtcagaagcg aaagctgtca aaaatgtatg tggtttatta aaacttatga gaaaagtagt
    35521 aaatgagtta gaattagatt ctactaacaa gaaattcttt ttaaaaccat ataaattaaa
    35581 atataataaa ttaaaaaaga ctactgaaag agaatatatc cagtttgatg caaaagattt
    35641 gcaaaaagaa tatagattat tatttttaaa taagtcaaga agaggtcaag atggagatgt
    35701 aattttacta agattttatg gtaagactgg tagattagaa gagataggaa ggtgtgagaa
    35761 ggtctacaga ggacaattgt catactaggt gatgactatg gaaataaagg atttgactaa
    35821 tgaacaggtg ataaatttta tggaatattt aggttcggac ttatctccta aaagcaatga
    35881 tagacagtta atattcaata cttgtttatg tcataatggt gattcataca aattatttta
    35941 ttatacagaa agcaaaacgt ttcattgcta tagttcttgt ggccatatag gtagcttaat
    36001 agatttatta atacacataa ataaatatga gtttaaagat gctattaatg aaataaaaga
    36061 tttctttggt atttcaaatc aaccaatgct aagaaaagga tttagaataa agaaaaaagt
    36121 agaacagata catgatatta aagatataca aatagaatta cttcctaccc ctaaaaagcc
    36181 atatgtatat aaaacttttc agcaagtccc aattgaagag tgggaaaatg aaaacatatc
    36241 ttttgaagtc cttaaaacat atcaagtata ttataatcta tatgaaaatc aaatagtaat
    36301 tccacatttc tgttggcatg atagagctag gcctgttggt attagagtta gaaacttaga
    36361 tgaagataag gctaaaagtt ttggcaaata tattccatta tggtacgaca ataggtgcta
    36421 taatcataga ttaagtctta atctatatgg attaaatgta aataaaaaat ctatcaaaaa
    36481 ttccaaaaaa gttattgtat ttgaaggtga aaaatcggtg ttacaaatgg ctactatgta
    36541 taaaaataat ccctctgtag ctatatgtgg gagtaatttt agtagagagc aaaaaaagat
    36601 attaatagat ttaggtattg aagaattaat catagctttt gatagacaat ttaaagttaa
    36661 agatgatgaa gaatatgtta tatggagaga taagatatat aaattagttc aagatattaa
    36721 agatgtagtg aatataagtg taatatggga taaatataat ttattgggct acaaagatag
    36781 tcctagcgat aaaggaaaga caatatttga aaaactatta aaaaacagaa ttaatataga
    36841 gaactttgtt aaacaataaa aaagtaaaga aattaatttt atgagaaaat ttttattgtt
    36901 tagggattta ttagcagatg ctaaataaaa tatgtgtttt aatatgaatt ggaggtagat
    36961 tacatgataa aatttttaat aaaaaacgta cacatgggag ataaattata ttgtataaat
    37021 aacataggaa attataaaaa aggtgaaatt tatacagtga gtgatactag tttttttgga
    37081 ctctttcata aaactaaagg agaaaattgc ttaccaattg aatgttattt tatacactta
    37141 ccaaaaaaag aagagttaga aagtaatcct aaatcaatga ctaaagaaga aattgaagat
    37201 gaactaggat ataaaattaa aatagtttct aaataataaa gtgaggtgat attatattga
    37261 gtatcttcaa taaaaaatat ggtagaaatg taaaaataac agaagaagaa cctccttctc
    37321 ctcctcccaa acaaccaata tataatagag ataatataca atatgataaa gaaatggaaa
    37381 gaaaactatt agatattgat aggttaaaaa tagaattgga aaaagaaaaa aagatagaag
    37441 aagaaagaaa aaaagtagaa agagtaaagg aaaatgaggg aaaggaatgg gcttgggttg
    37501 aaggatataa atgtacagat aaaaatatga aatgtaagga atttcaatat gaattaaaca
    37561 aagcctatag tatggatggt gatgatatta atatatggaa ttggggattc catttatgtt
    37621 tagacttggg tgacgtattt caatattatg ctccaaattt aagcaataga ttttttaaag
    37681 taaaagctct agtgaaaaaa gccgataaat ataaatatgg taaagaatat ggtgaagtga
    37741 ctctcctttc aggtagtgtt ttacgtggtc ataaaataaa taaaatagct gctaaagaga
    37801 taatattttt agaagaatta acttttgata aattaaaact acatatacaa aatagttttc
    37861 ctgaagtaaa cactaagtct aaatggtatg ccatatgtaa attaggagaa caagaatatt
    37921 atcatagaat gtttatgaat caaatgaaaa agattagatt ttcagataca ttttctcaat
    37981 tattatttga tgaatgtcgt aattattgtg aaatattaga aatcttaaat aaagcaatag
    38041 catattcaaa agaaaattta tctaaagata tgataatata tttgcttatg aaagatataa
    38101 gaaagtaaca tataaaaaga agggagataa tgtggacaaa ataaataaga tagatttgat
    38161 taagttaaaa gaaatgaata tatatgataa ttatcaatta aaaaaaatag caagtaaaat
    38221 gatattacat ctaagatatg aaataatgga cttttgtggt tgtggttctc ctgaagatat
    38281 atcttttatg ataaaaggag tattgactgc catacaaaat aaagaaaaaa attgtaattt
    38341 agaatacaca gaaagatgtt acatatttga aatagaattt aataatgttt gtggaataat
    38401 tggtagtaaa aatgatttga tacaagaatt tatattaaat gctttaaatt catatgggtt
    38461 attagaacat ggtagtagca tttgggggtc atggctatct gattatggta aacaaatatt
    38521 atgtgctttt gaaatagttg gtgactgtat tttagatgtt agctatttag attaaaaagt
    38581 tagttttaat aggaggtggt aattattaaa tatgtaatga gtgacttaca tggtatgtat
    38641 gataaattta tttcaatgtt agagcaaatc aatttcaatt caaatgacca tttgtatatt
    38701 ttaggagatg tattagatag aggggataag tctttagaga ttatagatta tataagaaag
    38761 tataagaata taactcttct aaaaggtaat catgagttaa tgtttcaaga aagttataca
    38821 gatgtaaata gtagttttct ttggttttat aatggaggta aagatacatt ttatgattta
    38881 ggaatgaagt cttatgaata taaagaaaat ttttataaat atattaagaa ccttccatat
    38941 ttagagataa tagataattt tatactagtc catgctggac tataccttcc aaataactat
    39001 gagaatctca gtatagagca aataattgag ttacaagaag aagatatttg tgtgtgggat
    39061 agaacaatat taaatactaa taaacatata aaaggataca caattattat tggtcataca
    39121 ccaactcaaa acattaaaga ttatgaaaag gctgaaatat ataaacagga aggaattata
    39181 aatatagatt gtggagcatg ttttgataat ggtaaattgg cttgtttaag attggatgat
    39241 atgaaagaat tttatattta agaggtgtta tatggacata ttaaaaatca gttacaacaa
    39301 tctaaatgct atatcaaaaa tatcagaagt attaagggat attgtaaatg aggacacttt
    39361 aatagtatgt ataggtagtg atagagtttt agctgactca ttagccccta tgattggcag
    39421 tatactagaa aaatctacta ttagaaataa aatatttgga gtattaggag attcaataca
    39481 tgctttaaat ttagaacaaa aaatacaagc aataaaaaat aattatccaa attcaaatat
    39541 aatagccatt gatgcttgta taagtaaaat ttcagataaa ggaacaatta taattagcaa
    39601 taaacctgtt aaaccaggtt tgggggtaga taagaagtta ctagaagttg gtgattattc
    39661 aatagttggg attataggta gaaataaata tgatatatat gatacttcag accctgaact
    39721 tatacttgat ttagctgatg ttatatcgaa atctttaata tctatattat tagaaaaaga
    39781 agaaaggatg attgtatgat acaggaagtc agacaagaaa tactattaaa aaatggtttg
    39841 attttatata caggtgattt ggtagaaata aaatacaaat cagatgaaga tgtaatagaa
    39901 catacttgta aaggtaaaat taaagaagca aaagaattat ttattaaatt agatacaagt
    39961 aagaaatata aaacttctga aagaatgatt tattcttggg aattaaaaga aataaaaaag
    40021 gtggatgatg aagatgaaaa agataattaa tagtaaatat agagcattat atttatttat
    40081 attttttcta attttcaatt taatatatag tttatatttt ggaaggaata cggagattgg
    40141 attttattct agtacatctt gcatagaaga atatatactg gatattataa cctctatagg
    40201 actatttttc tcaatgatgt tagctgggtt tgatgttaca gaaaacttta taaacagttt
    40261 aagggattat tgtgaagaag atgcaaagtg aggtgaataa attggaatta aaggattata
    40321 taagtaaagt agaattaaat gacatgccat tgaccttaga caataaatac atgttaaatg
    40381 aaataagcat atctgaagat agcaaaatct ttgctgtaaa agacacatca ggaataacat
    40441 cttattatac tgaaatagaa tttaataaac tatttagagg aattaatagc atcctagaga
    40501 atattatcta atatatattt ttaaggaact gagggttgag agctattaat taatagctct
    40561 ctattttttt gaaaggggag aattaatgga aaaggaacta tacagcttta gtaaattaaa
    40621 tgcatttaac acatgtccat ttcagtacta tcttacatat gtaaaaaatt taaatcgaga
    40681 acaaagttgt tacggttatt atggcaatga attacatagt ttactagaac aattacaaca
    40741 aagaaaaata acaaatcaag aagctattca aaaatacaat gaagttatag aatatgcaaa
    40801 tctaatggat tacaattttc ctacaccaaa ttcaagaatt aattatttag aatgtatatt
    40861 acattatata gaaaattttg taccaattga atgtgataga tactatatag aagaatattt
    40921 tgagtttgat attaatggaa ttacaatgag gggatatata gacttatatt atataatagg
    40981 taataaaatt tatgttatag attataaatc gagtagtaaa ttttctaaaa aagacctacc
    41041 taaaaaatca atgcagctta tcttatatgc aatgtatctt aaagaaaaat atccagataa
    41101 aataattgaa tatgtagctt ttgatatgtg taaatatatg aataatgaaa aaggagtatt
    41161 aattgaaaga aataagattg atagtataaa tgattatgaa agagctatag taaaaattaa
    41221 atatactaaa gaactagaga aacaattaat tgattttgtt gttgatacag ttactcaaat
    41281 aaaacagcta gattccaacg atgagagcgt ttggtgcaaa agtgatgaca aaagtaatca
    41341 gtttttttgt aaaactttat gctctcatta taacaagggt tgcaaacata aggaaaacaa
    41401 gagttttaga aaatcaaaat cataataaaa tgtagatttt aataagattg gagagtgttt
    41461 aaatgaatat accaaagcaa gtaaaagtag gaggtttatt ctataaaata gaagagacag
    41521 aagaacctat tttagtaaat aatcaattat gttatggatt agctgattat ggaactgaaa
    41581 caataaaatt atctaataat ctacaatcag aaagagtaaa agaagctact ttcttacatg
    41641 agttattcca ttgtatattt agagatagat gtatagagag tgaagatgag gaatatttag
    41701 tagatgtatt aagcaaggga cttcatcaag tgattattga taatcctaat atatttgaac
    41761 ttaaggtgag tatagatgga taaattaaaa aatatatttg tatttttagg ttattctggc
    41821 tcaggtaaag atagtatagt aacagaaatt tcaaagcaat tcaatattcc aattctaata
    41881 tcacatacaa caagacctcc ccgtaattac ttagaaatca ttaataaaac ctattatttt
    41941 gtagataata aattctttaa gaaagaaaaa aataacttta tagaaatgag aaagtatata
    42001 gttcatgatg gcagcacttg gctatatggt atacataaat ctgaattaga gaataaaaaa
    42061 tatgctttga ctatagtaga tgcaagtgga tataaagcac tagaaaaata ttttacagga
    42121 acaaaaacta agttagtacc attttttata aacgcagatg aagatactat aagaaaaaga
    42181 ttaatagata gaggtgataa tcttaaagaa atcgaaagaa gattagttga tgataagatt
    42241 aaatttaaag agtttttaaa tagtgaaaac tacattgtaa tagcaaataa cttaagtata
    42301 actaatgcag tagagcaagt taaaatacat atgaaagagg ggattcaatg gtcttagcct
    42361 tagatgtatc catgtcctca actggatggg cagttataaa tagaaataag aatatattaa
    42421 aatatggaaa aatagtaact aaaaaagata aattcaagtc tgaagatgag cgaatgtgtt
    42481 atatctgtaa tgcaattcaa gagttaatta taactcataa tattcaaata gtcttaattg
    42541 aagaccaatt cacttcaaga aactctaaaa caattcttag tttaagaaaa ctcttaggag
    42601 caataatgag gactgtaaaa ttaaataata tagagattga atatatgtat ccagtttcta
    42661 ttagaaaata tctcctgaat aatggtaaag ctaaaaagga agaagtagct gcatatgtaa
    42721 gagaaaacat tattgatatt ggagaatata ttgataggac ttgtaaggct aaaacaagtg
    42781 atatatatga tgctatagca cttggaatag cttatttaaa caaacttgat actaatagat
    42841 aattttaaaa agggagatta aattaatgag tataaatgtt aaaataaaga aattaaatca
    42901 aaatgcaata gtgccatctt atgcaaaaga aggtgatagt ggaatagatt tatatacact
    42961 aaaagatacg gtgattccag ctaaatcagc agtagcaata tctacaggta tatcattaga
    43021 aattccatat ggatatgaaa tgcaagtcag acctagaagt ggtataagct taaaaggaat
    43081 gttttttaca agatatgtga ctatatatga taaagtgcaa cctaaaaaac aaattagaaa
    43141 aatttttgta gcacatccaa ctgtaagatt aggtacagtg gattcaggat atagaggtga
    43201 aattaaaata attacatata atgaagagga ttttgatatt ttaattccta aacatactaa
    43261 acttgcacaa ggagtatttc aacaagttcc aagagctata ttaaaagtgg tagatgaatt
    43321 gactagctct gaaagaggaa gtaatggatt tggaagtaca ggtatataga tgaataagta
    43381 tataatccta gaaaaatata attattacta tgatgacaaa tgtatgacat tatatcattt
    43441 agaaaatatc tatacaggat taagattttc tattgaaaaa attgaattag agtactataa
    43501 tatatcctag actaactttg ggaggagtat taaatgtaca gatatgatga ttatgtaaat
    43561 ttaataaatg acttagaatt accaaaagaa attaggaata aatgtgttag agttataatg
    43621 acactagaga acatgcagga tattatggta gtacataagt ttaagactta tgttgttaat
    43681 gaaaaatata ttgaaaaagt agcataaaat aattaagtca tctattaatt taggtgactt
    43741 ttttgcattt atgtcgaata tttcatctac ttcatattgt attagatgct ataattaaaa
    43801 tatatgtcga aaggtagatg gtgaggatgt ttaaggagat atttgaaaag gtatttatgt
    43861 cagaagaagt tagtaaaact gtaaaagaag ttaataatat tattaaggat actaagatac
    43921 aagtaaaagg tggatttgga gaacttagat tagattttat acttagcaga ttaggcaaag
    43981 attacataat aattaaagac attattgtac caggctcaaa taagactaca caaattgata
    44041 gtattgtgat ttctatgtat ggaatatttg ttatagaatg taaaaatttt agtggatata
    44101 tttacggaaa tgataaagat aaagtatgga ctcaaattgt aggaaaaact aaaaatacat
    44161 tctataatcc ggtaagacaa aattatgctc atattaaagc aatagaaaat ataataggaa
    44221 ataaatataa tatatattca ataattgttt tttcagacaa agctacacta aaaaatgtaa
    44281 tgataagtgg aaataatatt atagttataa atgaaagtga gatattaagt acaataagta
    44341 aatgtaaaga tgtgactatt gataaagaag aaataaaaat tatcagagat aaaatttttg
    44401 attgtatgaa aaccactaat caaaatacta gagagcatgt tagaaattta aaaaatatta
    44461 ctgaagagga taagtgccct agatgtcaaa ttggacattt gattaaaaga aaaggtcagt
    44521 atggtgaatt ttggggatgt agtcagtatc caaaatgtaa atacactaga aaataaaaaa
    44581 caacctctta aattcgattt taaggctttg taaaaattaa tgcttataat taatcctgtt
    44641 cggaaaatcg attgcttaaa atggaaaata tataaaaata atagcgtgat agtgtaaggc
    44701 atagaattgt gtattctatg tcttatttct ttacaactaa gtaatttcaa taattatata
    44761 aataaaagaa ttaaaaaagt cagttgaaag tcagctaaaa tgactatttt ttgcctcctc
    44821 tgtatggtat aataagtata tgaattttca taggagggat aaccatgaac gcttttataa
    44881 gaaaaagaaa taaaaattat gtagtgtact tagaatttag agatgatgaa tcaggaaaaa
    44941 gaaaacaaaa aaatatggga gcatttgata aaaaaagaga tgctaataaa agattggctg
    45001 aagttaagga cagtatatat aaagatagtt ttcttgtgcc aaatgaaatc actctagctg
    45061 gatttttatt agactttctt gaaaaatata aagataatat ttcagcatct acatataaaa
    45121 gctatattgc tatttgtaaa aatcatatta atccttctat tggaaaatat cgtcttcaag
    45181 agttgagaaa tattcatata cagaattata tagatgattt agctggtaac ttaaaccctc
    45241 aaactattaa agtacatata aatgtattga gacttgcaat aaagagagct tatagaatta
    45301 aattaataaa agaaaatatt atagatggaa tagaaagtcc aagaattaaa aaatttaaaa
    45361 atgaaattta tgataaagag catatgctaa aattattaga agtagctaaa ggaactaatc
    45421 ttgagcttcc tattagtttg gctataggtc taggattgag actttcagaa gttttgggat
    45481 taacttggga taatattgat tttgatgaaa atacaataac agtaaataag ataactagta
    45541 gattagatgg ttctgttata cttaaagagc caaagacaga aagctctgtt agaaaaatat
    45601 ttgcaccaat agagcttatg aatttactaa aaaattatag gctggagcag aataagaagt
    45661 tattgagaag tattgttaga aatgaatata acttactatt ttttgataga aaagggaatc
    45721 caattgctga agatgtaatg agtaagaaat ttagaaaatt cttagagaat aatgacttgc
    45781 ctcatattag atttcatgac ttaagacatt cgcacgttac tttacttata aattctaaag
    45841 tacctataaa agttatatct gaaagggtag gacattcaaa tattaatact actcttaatg
    45901 tatattctca tgtacttaaa gaaatggaca aagaagcttc tgatagaata tctgaaaact
    45961 tatttaaggc taattaataa ttattatcaa taaatacaaa attaataata aaagcaaagg
    46021 tataaacata aaggggaatt tttacaaagc cttagaatgg catatagagc gtcagatttt
    46081 agaagatatt aaaattttat atttccaata tttatgatat aataaacata gcaaggaata
    46141 aatatttgaa aaaagctgtg agtggtgttt ccatagaaat ttcctcactt tcttatgaaa
    46201 ggaggtgaaa actatggaaa atctaatgat gagtattata gctggtgtta tagctagtta
    46261 tatctacgat aaattaaaat gccactctga gcgaccaact aagagtggct gggaacttaa
    46321 tattaagttc cataaaaaca aacattaata actaattaat ggaaacatca ctcaaagtta
    46381 agtaaaatat gtttccttgc ttttattata ccacaaatta agaatattaa aacaaaattt
    46441 tgtaaataca tacttaatta tcaatttaat catttggaaa aaattggatt tgattgtatg
    46501 aaattattgc tgtaatattt aaattaaata aacaaatgat gggagagata attatgtatt
    46561 taagaaaatt atcaaagtca gaattaattg taatgaaatt tatatggaat ttagatacaa
    46621 aagtaaaatc atatcaaatc attaattata tgaaagaaaa atatagttgg tcagaaaaaa
    46681 caacattaaa aactttatct aagttatcaa ataaaaggtt catatatgtt caagaaacaa
    46741 gtcaatgtac atactataca atttcaatta aagaagataa gtatcatgaa tttatatcac
    46801 aaaaaataca taagctctta gggtgcaatt ctataaaaag ccttttagca tcattgtttc
    46861 aagaagaatt aacagatgaa aaaataactt cattggaaga atgggtaaaa aactgggaag
    46921 aagaggaata aagataatat atactattcc ttaaaaatct atttaaagaa taattgaaat
    46981 tttgatttta atataatttc tttaatcaac tctaacatac aaaaataaga ctcttttcaa
    47041 tattaactaa agagccttat tttataattg attatatttt agttaacttt tgtgtatcaa
    47101 aactatactc atatacttct aatgtttcag aatttacaat tagagttcct actgttttaa
    47161 tgctatcttc tttatcagta taagtatcag aaccatcagt gctagattca tcttgtttaa
    47221 catttttaac actgattatt tcaaactcat agtgtggaaa atctatattt tcagcttttt
    47281 tatatcttga gttaccataa ttatatgtat aacaatattc atgtgctttt gaaccttttg
    47341 ctttatatat agcatctaaa gcttttgaga tgtcacttct tttagtatta ccagttaaca
    47401 tataatctgg agttaagcat tggtctaaaa ttcatttttt atatcaccta ttattattgg
    47461 gaaattagca ctagtaacta atgatttgtc attagtttca tttactaaaa atacaggata
    47521 ttctttgtgt attgtagaag ataatagtgc atctattaaa ttatcagaac ttacaataca
    47581 tatttgattt ggatgtttta attcatcttt ataaaaatgc ttaattattt tttcattagt
    47641 gtcaagtctg tcaacaccac caattctagt tgcttttgta ctgttcacta aagagtcgtt
    47701 tattgatgct gttcctccaa tagcatatgt ttttacccca tctgttttaa atggtatatt
    47761 ctttccattt gttaatacaa ctggattttt atatttagca gcaactggag atatacttat
    47821 agaatcagcc tctccttggt aagcatttgt aagatatatt tcatcaacct tttttagatt
    47881 gcttatttct tttgcaacat tataacttgt gtctattctg tcttttcctt ctatcctaat
    47941 aacctttatt tttttgtttt ttagtatatt ttctacattt ttgcttatag aattaactcc
    48001 acctattaaa tagatttttt taaatttatc taatctttta agagtagaat ttggtataga
    48061 attttgttta gtaagtaata ttggcgagtt agaagctcca gctaaaccac tagcactcaa
    48121 cccatctgca agacttttat ctgtgtttat aattattgct tgggtgtatt ttcttctgtc
    48181 tgctattaaa cctgctgttt catacttatt tgctccttgt attctatgag ctggaggtct
    48241 aaattttagt tctttagctg aaactaataa tgtgctagac atgacaatag aagatattat
    48301 agctaaggtt aatgcttttc ttttgaattt catattgtaa aatcctccca tccttttata
    48361 ctatgaatga tagtgtatat tattttagtt gtcaactctc tatgtatatt ttattatata
    48421 tataagaata aattgtatta attatatcgt tacgaaaact ttttgtatat aataaaagtt
    48481 cacttcttta tttattctta taacaatcct ctaaagaact aattaagctt tctatagaat
    48541 tagaatgtac ttcttctaaa aatctctttg tttcaaatat tttatattct tcttttgtaa
    48601 taagtattgt ataatgtgta attcttttta tcctctcaga atctaaaaaa cgtttaatag
    48661 tgagtcttga aagaagagtt agtgtagttg tcgccttcca gttatattct aattccatag
    48721 cttccataac ttcttttgat gttactatat cattcttatt ccatatgaat ttcataactt
    48781 ttaattcagc ttgtggtatt ttttgcatgg atatgtacat tcctttctgc aattaaaata
    48841 tcaacatata tagacattaa aataataaaa tatatgttat aagtatatat taacataaag
    48901 taaaagaaga tgtctcaatc ttaagacatc ttctttatta aatttttatt cttcaaattt
    48961 tcgatattca attatttctt ttatcaatct aattatcgct ttttgttctt ttactgttaa
    49021 actatcaatt aactcataag cttcagacga aagagtttca acatcatttt cattttgtat
    49081 aatattttca aataaaactt ctaaaggtat ctctaatgca tttgcaattt taaaaattga
    49141 acgaagtgat gctttacttt cacctctttc aatttgacca atataagcag gaaacaaacc
    49201 agcctttttt gctaatgtat tttgattata ccctgctttt cttctataat ttcttattct
    49261 tttaccaatt acattatata tttcatcttc ttctaacatt tttatcattc cttattagat
    49321 tatattagtt tatttatctt aatgatatgt tataca
//
LOCUS       DAENCV010000015        47601 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAENCV010000015 DAENCV010000000
VERSION     DAENCV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.015"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            150..1301
                     /locus_tag="KQD82_000820"
     CDS             150..1301
                     /locus_tag="KQD82_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379306.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNDFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1419..2057
                     /locus_tag="KQD82_000821"
     CDS             1419..2057
                     /locus_tag="KQD82_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG4379307.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2058..3080
                     /locus_tag="KQD82_000822"
     CDS             2058..3080
                     /locus_tag="KQD82_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG4379308.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3070..3690
                     /locus_tag="KQD82_000823"
     CDS             3070..3690
                     /locus_tag="KQD82_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379309.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3814..4500)
                     /locus_tag="KQD82_000824"
     CDS             complement(3814..4500)
                     /locus_tag="KQD82_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG4379310.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4514..5059)
                     /locus_tag="KQD82_000825"
     CDS             complement(4514..5059)
                     /locus_tag="KQD82_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379311.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5287..6534
                     /locus_tag="KQD82_000826"
     CDS             5287..6534
                     /locus_tag="KQD82_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG4379312.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6621..8615
                     /locus_tag="KQD82_000827"
     CDS             6621..8615
                     /locus_tag="KQD82_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG4379313.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8615..9937
                     /locus_tag="KQD82_000828"
     CDS             8615..9937
                     /locus_tag="KQD82_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG4379314.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10216..11502
                     /locus_tag="KQD82_000829"
     CDS             10216..11502
                     /locus_tag="KQD82_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG4379315.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11588..12895
                     /gene="grdB"
                     /locus_tag="KQD82_000830"
     CDS             11588..12895
                     /gene="grdB"
                     /locus_tag="KQD82_000830"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12635..12637,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG4379316.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDGAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     DEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13183..13914
                     /locus_tag="KQD82_000831"
     CDS             13183..13914
                     /locus_tag="KQD82_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379317.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13916..15274
                     /locus_tag="KQD82_000832"
     CDS             13916..15274
                     /locus_tag="KQD82_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG4379318.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTRGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15313..15798
                     /locus_tag="KQD82_000833"
     CDS             15313..15798
                     /locus_tag="KQD82_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379319.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QRKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(16061..16297)
                     /locus_tag="KQD82_000834"
     CDS             complement(16061..16297)
                     /locus_tag="KQD82_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379320.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(16452..16676)
                     /locus_tag="KQD82_000835"
     CDS             complement(16452..16676)
                     /locus_tag="KQD82_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG4379321.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16773..18026)
                     /locus_tag="KQD82_000836"
     CDS             complement(16773..18026)
                     /locus_tag="KQD82_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG4379322.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLNNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18286..19275
                     /locus_tag="KQD82_000837"
     CDS             18286..19275
                     /locus_tag="KQD82_000837"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG4379323.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19347..20087
                     /locus_tag="KQD82_000838"
     CDS             19347..20087
                     /locus_tag="KQD82_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG4379324.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20303..21454
                     /locus_tag="KQD82_000839"
     CDS             20303..21454
                     /locus_tag="KQD82_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG4379325.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21479..22234
                     /locus_tag="KQD82_000840"
     CDS             21479..22234
                     /locus_tag="KQD82_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG4379326.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNNNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22426..24813
                     /locus_tag="KQD82_000841"
     CDS             22426..24813
                     /locus_tag="KQD82_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG4379327.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNSNI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(25044..25277)
                     /locus_tag="KQD82_000842"
     CDS             complement(25044..25277)
                     /locus_tag="KQD82_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG4379328.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            25566..26156
                     /locus_tag="KQD82_000843"
     CDS             25566..26156
                     /locus_tag="KQD82_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4379329.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            26270..27115
                     /locus_tag="KQD82_000844"
     CDS             26270..27115
                     /locus_tag="KQD82_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379330.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKLKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTAECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            27112..27795
                     /locus_tag="KQD82_000845"
     CDS             27112..27795
                     /locus_tag="KQD82_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379331.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IVIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            27792..28508
                     /locus_tag="KQD82_000846"
     CDS             27792..28508
                     /locus_tag="KQD82_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379332.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMLGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            28605..28868
                     /locus_tag="KQD82_000847"
     CDS             28605..28868
                     /locus_tag="KQD82_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379333.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            28840..29295
                     /gene="def"
                     /locus_tag="KQD82_000848"
     CDS             28840..29295
                     /gene="def"
                     /locus_tag="KQD82_000848"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG4379334.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            29347..29808
                     /locus_tag="KQD82_000849"
     CDS             29347..29808
                     /locus_tag="KQD82_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG4379335.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            29858..30760
                     /locus_tag="KQD82_000850"
     CDS             29858..30760
                     /locus_tag="KQD82_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4379336.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            31130..31942
                     /locus_tag="KQD82_000851"
     CDS             31130..31942
                     /locus_tag="KQD82_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4379337.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            32989..33750
                     /locus_tag="KQD82_000852"
     CDS             32989..33750
                     /locus_tag="KQD82_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG4379338.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            33815..34597
                     /locus_tag="KQD82_000853"
     CDS             33815..34597
                     /locus_tag="KQD82_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG4379339.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            34864..35283
                     /locus_tag="KQD82_000854"
     CDS             34864..35283
                     /locus_tag="KQD82_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG4379340.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(35386..35652)
                     /locus_tag="KQD82_000855"
     CDS             complement(35386..35652)
                     /locus_tag="KQD82_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG4379341.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            35879..36580
                     /locus_tag="KQD82_000856"
     CDS             35879..36580
                     /locus_tag="KQD82_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379342.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            36662..37270
                     /locus_tag="KQD82_000857"
     CDS             36662..37270
                     /locus_tag="KQD82_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG4379343.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            37578..38042
                     /locus_tag="KQD82_000858"
     CDS             37578..38042
                     /locus_tag="KQD82_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG4379344.1"
                     /translation="MKKLVISLISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNACLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            38679..39695
                     /gene="gap"
                     /locus_tag="KQD82_000859"
     CDS             38679..39695
                     /gene="gap"
                     /locus_tag="KQD82_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG4379345.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            40090..41259
                     /locus_tag="KQD82_000860"
     CDS             40090..41259
                     /locus_tag="KQD82_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379346.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            41640..45893
                     /locus_tag="KQD82_000861"
     CDS             41640..45893
                     /locus_tag="KQD82_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG4379347.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATEEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            46042..46536
                     /locus_tag="KQD82_000862"
     CDS             46042..46536
                     /locus_tag="KQD82_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG4379348.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            46652..47029
                     /locus_tag="KQD82_000863"
     CDS             46652..47029
                     /locus_tag="KQD82_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG4379349.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            47235..>47601
                     /locus_tag="KQD82_000864"
     CDS             47235..>47601
                     /locus_tag="KQD82_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG4379350.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNAD"
BASE COUNT        19148 a         4802 c         8051 g        15600 t
ORIGIN      
        1 ataaattagg tttatatttt ataaaaattt gctaaaaaaa acaattaaaa attgtaaaga
       61 aaaaagatat aattagaact gtagtaataa atgttttatt atagataata gagttttaag
      121 tatcttaaat aatatttgga ggaatatgaa tggaattaag accaagaagt gcaaaattag
      181 aagaatttga taaagtaata gaattaataa actatgtatt tagaatatca agaaatcata
      241 aaccaactat gatggaagaa tttcctcttt tactaagtaa aaataatata gaaaatatga
      301 ttataatatc agaagatgat aaagtggttt ctgatgtaaa ctatctaatt caggatgtat
      361 ctattcaagg aaacagattg aaggttgcag ctataggtgg agtatgtact catccagatt
      421 atgaaaagag aggctattca tctaagatat tggataaagt agaagaaaaa atgttttatg
      481 atggtgtaga tattgtaatt atatcaggaa caagaagtct atacagtaga agaaattgta
      541 gtttggtaaa gagtttttat aaatatacaa taaaacctga agatgtaaaa atagcatatg
      601 aaatagttga atttgatgaa actaattttg aaaaagataa tgatttagat aaaatgattg
      661 aactatataa tcaaaatagt actagattta taagaacgag agatgagttt caaaaactat
      721 tgcatgcagc aactattgcc tggggaccaa ttggttacaa gaaggtattt ataaaggaaa
      781 ataataatat aataggatat ttgataataa gaacaataaa aaaagaagat tctactgtag
      841 gagaagttgc tgaaataggg ttaaactctg taaatgttga aaatatattg aaatatgttg
      901 caaataaatt tggattagaa tatttaaatt ataaagtaca tgtgaaaaat cttaaagacc
      961 agttgaaatg taatgggaca aaatctttgg attatcaaca aggaactatg aaaataataa
     1021 attttactaa gttgtgtgat agtttaagaa gttattttag tcaatatgta gattttgaat
     1081 tactaaaata catggaattt aaacaagttg aaaataaata tattattaaa tacaaagaag
     1141 aagaacttgt aatagaaaat ttggataagc ttaataaact gttttttgaa aaaaatgagg
     1201 aacagtacaa tgactttaaa catctaaaaa atatatatga atttgctaca aaggcatttc
     1261 ctgtagattt tccatggact gcaaatttaa attatcaata gttttagatg atattaaata
     1321 aaattgattg atacctagag ttagtccttg ttaaataaga gaaattattg gatattgaaa
     1381 aagcaatttt gtaagtaatt agaaaggatg agtattatat ggacatactt gatgatatag
     1441 cagaatgtat attaaatatg ggtgtagata atatagagaa tttagtaaaa attgctatag
     1501 ataaaaatat tgatgtggaa gatatttatg aatatggttt aaacaaaggt atgataggag
     1561 ctttagataa gtttgagaat aaggaatact atctttcaga agttatagta tgcacagatg
     1621 cattaaataa aggtattaat cttttgaagg gaactggaaa agtaaagaaa aaatcaaagg
     1681 gagtaattct gatgtctgta gtagaaggag atactcatga gataggaaaa aatattgtaa
     1741 aagtaatggt agaagccact ggatataagg ttattgactt aggtgtaaat agaaaaagtg
     1801 aagatataat agaagaagct ataaaaaaca atgtagatat aataggctta tcttcaatga
     1861 tgacaactac aatggaaaat atgaaatcgg ttatagacga acttaatatg attgagatag
     1921 ataaaagacc aaaagtaata attggtggag gacctgtaag tatggagttt gcagaagaaa
     1981 taggagcaga tggatacagt tcaaatgctc caaaagcagt taaattaata aataaactta
     2041 taggaggtga agtttaaatg ttttcagata tgactttact tcagtatata aatagtgaag
     2101 atagaggttt ttttctgcct gatatgggta caaatggatt gttcttaata aataaaaagg
     2161 catatgaagt atatgatagt gtagatttgc aattagaatt agcaaagact atggacaagt
     2221 attttgaatc agattttata tactcattct gtgatgggat tacattttgt gaaactctgg
     2281 gtttagagac attaagacca gattatgatt ttccaagtgt attgacacat actataactg
     2341 acagagataa attaagaaaa ttagatgttc cagaccctta tacgagtgga cgtatgccac
     2401 tcaatataga gagcttatca gttatatcaa aaagtattaa aaaaccacta tatgaatcga
     2461 tacaaggtcc atttacactt gcaggtcaat tagctggagc tactcaactt ttaagatgta
     2521 taataactga taaaaaattt gtagaagaat tattggaatt tacaactgaa ttggtaagaa
     2581 gatatgctgt agctgcaaat aaagcaggag caaaatatat ttcaatagca gagccaacat
     2641 cagtaaccct aagtaaagat agatttgatg aatttatagt aaaaaattta aataaaatct
     2701 acaatgaact tgattgttgg aaaggtatgc atatatgtgg agacaccaga gaattattag
     2761 ataatatgct aagttgtaat atagatgcag taagtttaga ccaaatttta gactatgaag
     2821 aaattgctcc aatgattcct agagatatag ttttgattgg taatttagac ccaataaaac
     2881 ttcttggacg ttctaagcca gataagatta gaagagagac actaaaattg ttaaagaaga
     2941 tgagaggata tgataacttt ttatgtgatt ttggctgtaa ctgcttaaat accactccag
     3001 tagaaaattt acaagcagct ataaaggcag gaagaattag ttataaagaa ttagatagga
     3061 ttgatattga tgaattataa tagtaataaa aataaatatc ttttaaagga tataaattta
     3121 gatgatattt ttatagataa gaatattgtg ctaaagtttt tggggtatgg aaatagaaaa
     3181 gctccagata caattagtaa aatcgttgaa agagaaataa gaaatataaa tgatatttta
     3241 gatatcaaga tatatataag tgaagttgat attaataaaa cacctaaaga atgtaaaaaa
     3301 gcatttgctg tactatatac aataggggat aaaattgata ttaagatgaa tgactatatg
     3361 gaaaattgca atatgatggc agggctagca ttggataaaa taggtatagt atgtttagat
     3421 tgtattaatg agaaaataaa aatgtattta aaaggaaagt atgatagtct caaaatatcg
     3481 catgaaatat atccaggaga taaagatttt gaagtagaga gacaaaagga tatttataac
     3541 tatgtaaaaa ataaatataa tagtatagaa atagaaatca atgattatca tcaactatcc
     3601 cccataaaaa gtgttgcaat gcttatttta atgggggatg aagaaaattt agaatcaaga
     3661 tgtagcaaat gtcctagaaa atgcttttaa ctagttttca agactaagat ttgttttgtg
     3721 tatgaattgc aataattttt ttggtcattt tatgtataaa ctaaaaaaat tattgcaaag
     3781 taatattcta tttaatttga acagacttac aaactatagt agtttcttta attcaataag
     3841 tgtatttatt tcataagttg gtatatgatt agtataattt atactgttat tggaatttaa
     3901 ccaacaagta tctattcctg aatttattcc acctaagaca tcagaggtta agctatcccc
     3961 aatcattaag gcagaagatt tatcatgaca attaaacttt ttgagagcgt atttaaatat
     4021 ttcaggattt ggttttgata ttttcaactc ttcagataca accattccat caatatattc
     4081 ttttattttt gaattttcaa gtcttttctt ttgaactgca gaaattccat ttgttattat
     4141 aacaatagta tacttttttt taagatattg taatatatcg attgcacctg gaattaaaaa
     4201 tgtacattca cctaaaaaat ttaagtacat tttgctaaga gtttcagaat caacatccaa
     4261 agatatttta tttgcaaaaa gttcaaatct aagtaattta agttcattca aagttataat
     4321 atttttttca aggtcgagcc aaagcttatc acttatattt ctatatgact ctatataatt
     4381 ctcaaaatta aattctaaat caaactcaga tagaagtttt ttaaaagcat gtgattcact
     4441 tttcttaaaa tcaaataaag tatcatctgc atcaaaaaat ataaatttat atgtcttcat
     4501 ggtttttact cctttacaag atgactgcaa aaattacttt actttcacta tttgagtcat
     4561 tttgccattt atgttttgtt cctaaaggaa cttttacact atctcctttg gacaaagtaa
     4621 attcatcatc atccataaaa agtttaactt ctccctccaa aacatatgca atttcatctc
     4681 cattatgagt aatcctatca acacaagaag aagtgtttgg agctaaatcc ataattgcaa
     4741 attctaaatt accactagag tctggaatta aaatctcata tataacttct ttgcttccag
     4801 gtaaaataac tttttttcta ttatcatgtc ttactatcaa gtttttttta tctacttcag
     4861 atacaaaaaa tgtaaataaa ggaacattta gagaagaggc tatagatttt aatgaattta
     4921 cagatggatt agcaagacct cgttctattt ggctcaataa tgatggtgta acatcagcta
     4981 gttttgctaa atctcgaata cttaagtttt gtttttttcg aacttcagct attttttccc
     5041 ctaaatttaa atcattcata taaatatcct ctctttttaa tttaaaaaat attaacttaa
     5101 aataatcaag taaaatgatt atgtagtatg aaattattat aacatataga attaggtttt
     5161 gaaaaaacta tgttcaatat aattaaataa attaaaatat aattgacaaa gtggatacag
     5221 aatacttaaa atatatttaa ataaaattaa gcaaaagata gttaatttat gataggaggg
     5281 gatattatgc aaataacgac ttttggagct atatttggtt tattaatagc aattatttta
     5341 attataaaaa aatttcaagc tgtatatagt ttaatgttag gagcatttat tggaggatta
     5401 gttggaggag caaatataac acaaactgta gattttatgg caaatggagc tatgaatata
     5461 tcaccatcaa ttcttagagc tttggcatca ggtgtattag ctggaagttt aataaaaaca
     5521 ggggcagtag ataaaatatc agaacagatt gtaaaaatat ttggtgaaaa gagggcttta
     5581 ttttcgattg ccatttcaac tatggtactt gctggagtag gcgtaaattt ggatgtatct
     5641 ataataactg tagcacctat aggattatat ataggtagaa aattaaatta ttctaaattg
     5701 tcaatactat tagctatgtt gggagggggt aaagcaggta atataatatc accaaatcca
     5761 aatactatag cagtagcaga taatttttct gtcaatctct ctagtgttat gatggcaaat
     5821 ataattccag caattattgg agttgttatt acagttattt tagcaagtat attaataaat
     5881 aaaggaaata aagtacaatc atatgaaatt ttagagcaaa gagaagattt accaagttta
     5941 tttaaatcat tatgtggtcc aattatagct atatttctat tgtttttagg aaatgtatct
     6001 cctatagtta tagacccaat gatagccctt ccaataggag gaatcgtaac tctaattgtc
     6061 acaggaaact tgaacaattc aagagaatat ttagcttttg gattatctaa gatgcaaggg
     6121 gtatgcatat tattacttgg aacagggact atcgcaggaa ttatacagat gtctgaatta
     6181 caacagagta caataggtgc tttacaattt ttaaatatgc cacaattttt attggcacca
     6241 gtttcgggta tacttatgtc attagcaaca gcatcatcaa cagctggtgc tactatagca
     6301 tcatctactt ttcatgatgc tataataaat ggtgggctat caccgatttc aggtgcatca
     6361 atagtaaatg ctgggtctag tgtattcgag caattaccgc atggctcact atttcatact
     6421 agttcaggta gtataaacat ggatataggt gaaagattta agttaattcc atatgaagca
     6481 ttgataggaa tagtaatgac tataatttca acatctattc aattagtttt ataataaata
     6541 taaatattta atgaaaaaag taggattttg ttattaaata ttgaatttat tatagtaaaa
     6601 aagaagattg gagttttaaa gtgaataaaa aaaagattgt tattataggg attatttatt
     6661 catttttagt agtattttca cttacaaata tgtatgtaaa tatggagtat aatttaaatg
     6721 tatttgaata tattaagaag tcacttccat ttacagaaga agaaaaaaag tggttagaaa
     6781 aacacaagaa tttgatttac agctctgacc aaagttcacc tcctttgaga tataaaggta
     6841 aggaagatgg acagtataag ggaattattg ttgatttaat aaattctctg tcaattcaaa
     6901 ttggtagaga tttttatttt aaaccaaata attggtggaa agaatctttc gtaaattcaa
     6961 tagatgatag tataaagttt tttgacttga ttccatcaaa agaaagggca aataaattta
     7021 tatttactga cccaatatac actttgagtg caaatatatt gaaagataaa aagtcccagg
     7081 atataaatag ttacatggat ttaaagggga aaacagtagc aattccagaa ggtgattatt
     7141 ctataaattt tttaaagcaa aaaattcaag atataaacat tttattgact ccagatataa
     7201 agactggagt caaccattta atgtctggaa aagtagacgc agttgttggt gatgaaccag
     7261 tattgcgata ctatataaat aattatggac tttcaaataa atacagtgtt ttaagtaatc
     7321 ctatatatac taaaaaagca gttcttgctg tacctaaaca atatgaagag ctggttagta
     7381 ttttaaataa aggcattttt aaacttcaga aaaatggagt gtataaagat ttaaagaaaa
     7441 aatggtattc tacgtataat gaagttgatg atatattgta tgagcgagga attgtgccta
     7501 gtatttatct ttttataggt ataattttaa tatcaattta tgttttttat agctatacat
     7561 atttgttaaa gatagaaatc aaaaggaaaa cagaacaggt aatagaaaat aaaaaaactt
     7621 tagaggcaac ttttaatagt attacagata taataatgct tgtagatgaa aataacaata
     7681 tagtagaatc gaacaaagta ctatatgact ttatgggtga aatgagttat aagatagcag
     7741 atttgattag catgataaaa ggtgttattg aaaatacctt ctctgaaaat actaataaaa
     7801 ctagtgaaat agaaatacac aataaaattt taaagataaa cacttttcct gtagagtata
     7861 aaaaaaataa tactgagtat atagtagttc ttattaaaga tataactaat gataaaatag
     7921 tagaggcgaa attacttaga gaaaacaaaa tgatatctat aggacaatta gcttctgggg
     7981 tagcacatga gataagaaat cctttgggta taataagaaa taattgctat ctcttaaaag
     8041 ataacgttac aatggaagaa gtaaatgatt gtgttaaatc aatagaaagt aatgtagata
     8101 gagcaagtaa tataataacc aacttgttaa atttcgcaag aatatcagat gataatttag
     8161 aacatataaa tataagaaat tttattgaaa atatagtgaa acttcaatat aaaatgttac
     8221 agttaaagaa tgttgaaata aaaattgact gtgaacataa tttaatatgc tatataaatg
     8281 gagaatcgtt aaaacatgta tttataaatt taatttctaa ttcaatagat gcaatacatc
     8341 aagatggaaa aattattata tactgttatg aaaaaaatca ttgtttattt atagatttta
     8401 aagataatgg tgaaggaata aaagaagatg ccctaaagga catatttaat cccttctata
     8461 caacaaaacc aattggtgag ggaactggtt taggtttata cattacttat aatgagatta
     8521 aaaagaataa tggagatata agtgttgaaa gtaaattggg ggttggaact tgttttcata
     8581 taaaaattcc attaaataaa gaggtgacta tataatgaaa atactattag ttgatgatga
     8641 gctagaatat ggagtggtaa tgaaaaaaat tctacaaaag aagggctatc ttgtagatat
     8701 agcactaagt ggtgaagaag ctataaatat aattaaaaaa gataaaaatt atgatttggt
     8761 tttatctgat gttatgatga aaaatatgga tggagtacag ctattagata gaattaaatc
     8821 tattaataga gatatagagg ttatattagt tacaggatat ggaagtattg aaaatgcagt
     8881 tgatgctatg aaaagaggag ctttatctta ttttataaaa agtaatccca ttgaaaattt
     8941 attagaagaa gttgaaaagg taaaggcaag taaaatatct gtttctgtac aaaaaaataa
     9001 tttggaattt actttggaat ctaaaaatag agattttaat aatatcataa aaatagcaaa
     9061 aaaagctgct tgtaaagatg ttaatatatt aatcttggga gaatctggtg taggaaaaga
     9121 tatattagct agatatatac atagcataag tccaagaaag aatgagatat ttgtacctgt
     9181 aaattgttgt tctttttcag aaaatctatt ggagtctgag ttgtttggtc atgaaaaagg
     9241 ttcttttaca ggagcagtag atagtagaaa aggtagattt gaactttcga ataaaggaac
     9301 tctatttttg gatgaaatag gagatatacc tctaaatgtt caggtaaaac ttttgaggac
     9361 acttgaggac aaatctatag agaggatagg aagtaataaa tctattaaag ttgactttag
     9421 gttaatttgt gctatgaata aagaaccaaa agtggaaata tcaaatggaa atataagaga
     9481 agattttttt tatagaataa gtacaataac aataactata ccaccgctaa gaaagagaag
     9541 agaagactta actactctta tagaattttt cttaaataag taccaaattg aacatgataa
     9601 aaagatacac tcgattgata aggaagtaaa ggatttttta ttaaattaca attatccagg
     9661 aaatataaga gaattaaaaa atataataaa tagacttgta gttttaagtg aagaaggcaa
     9721 tttatcaaaa gacaatttaa atttaatatc aaataatgta tacatagatg ataaaataag
     9781 tattagacct ttaagagaaa taagaaaaga gtttgaatgt gagtatattg aaaaagtttt
     9841 atctttgtgt ggaaataata tttcaaatac agctaaaaaa ttggagatta gtagaagaca
     9901 gttaaccaat aaaatatcag agtataatat taagtgagaa aaaaacttct cattaagtga
     9961 gaaaaaaatt tctcaaactg tattaaaaga taaacagaaa aaatctgttt atcttttttt
    10021 attataattt taaaaattaa tatttacaat aattattggt aaattcaata ttggtgtaaa
    10081 ttaaaaatga aaatttctat gacattaata catagaatat atattttaaa taattggcat
    10141 agaaaatgct ttatatatta ataaacgtaa taaatacttt taattgcgat taaatatatt
    10201 aagggggatt ttcatatgaa gctagagcta gggaatatat ttataaaaaa tgttgaattt
    10261 ggagaaaaga cagaagtaaa agatggagta ctatatatta gtagcgaaga aatagaaaag
    10321 atagtattag aagatgaaag gattgtaagt gtaaatgttg aacttgcaag acctggcgaa
    10381 tcaataagaa tagctccagt taaagatgta atagagccta gagttaaaat tggagatgaa
    10441 agcaaaatat ttcctggaat tataaataaa gtgaagactg taggaagtgg aagaacccat
    10501 gtacttttag gagcttgtgt tgtaacttgt ggaaatatag ttggatttca agaaggcgta
    10561 attgatatga gtggtcctac tgctaaatat acaccttttt caaaaacaaa taatatatgt
    10621 attgttataa aagcaaagga tggtatagat actcatgatt atgaagaagc agctagaatt
    10681 gcaggattaa aaataggtgc atatgtaggt gaggcaggaa gagaagttga gccagatgaa
    10741 gtcattgtat atgagacaaa gccattatta aaacaagtaa aagaatatcc agacctgcca
    10801 acagtagcat atgttcacat gttacaatca caaggacttt tacatgacac ttattactat
    10861 ggggtggatg ctaaacagat agtgcctaca tttatgtatc caactgaaat aatggacgga
    10921 gctataatat ctggaaactg tgtagcacca tgtgataagg ttacaacatt ccatcattta
    10981 aataatccag ttatacatga cttatataaa agacatggaa aagatttaaa ctttatagga
    11041 gttattttga ctaatgagaa cgtattctta gtcgataaag agagaagctc tgatatggta
    11101 gctaaattaa tagaattttt aggagttgat ggagttctag tgacagaaga aggttatggg
    11161 aatccagata ctgacctaat gatgaattgt agaaaatgta gtgaagttgg agcaaatgta
    11221 gttttgataa cggatgaatt tcctggaaaa gatggcaaga gtcaatctat agcagacgct
    11281 actaaggaag cagatgcagt tgtttcttgt ggtcaaggaa atctagtggt tcatttccca
    11341 gcaatggaaa aaattatagg aactttagat tatgttgaaa tgatgatagg tgggtataaa
    11401 ggatgcttaa atgaagacgg aagcatggat gcagagctac aaattataat agcatcaact
    11461 atagcaaatg gctataatca tttgacagca agatattatt aaataagtat ttgaatataa
    11521 agttctgttg atgtaatcat tggtctaact gatgtaataa ctataaagtg atttggagga
    11581 ttaagagatg agtaaaatca gagtggttca ttatataaac aatttctttg ctggtatagg
    11641 tggagaggaa aaagcagata ttccgccaga aaaaagggct ggtgtagtag gacctggaat
    11701 agcatttcag agtcaattta atgatggtgc agagataatc tcaactgtaa tttgcggaga
    11761 tacgtatttt ggtgaaaata ttgagagtgc aactcaagaa cttttaaaaa tgataaaaga
    11821 agaaagccct gatttattta ttgcaggccc agcatttaat gcaggtagat atggagtagc
    11881 ttgtgggact atttgtaagg cagttgaaga agagttaaat attcctgtaa ttacaggcat
    11941 gtataaagaa aatccaggtg tagatatgtt taaattagat ttgcatatag tgtctacagg
    12001 aaattcagca gcaagtctta gaaaggtagt tccaataatg acgaaactag gtcttaaatt
    12061 ggttagagat gaggaaatag gacctcctga agaggaagga tatataatga gaggaataag
    12121 aaaaaatttc ttccatgaac ttagagggtc tgaaagagca attgatatgt tggttaaaaa
    12181 gatgaaaggg gaaagctttg aaactgagta tccaatgcct gaatttgata gagttacacc
    12241 tatgaaacct gttaaagatt tatctaagat aaaacttgcc ctagtaactt ctggagggat
    12301 agttccaatt gataatccag acaaaattga atcttcaagt gctactaaat atggaattta
    12361 cgatttaaca gatatggatt ctatgtcaaa taaagaattt acaactatac atggggggta
    12421 tgatagagca tatgtccttg aaaatccaaa tttagttgta ccactagatg tagttagaga
    12481 gcttgaaaaa gaaggtgtaa ttggagaatt agtagattat tttataacta ctacaggaac
    12541 aggaacgagt gttggcaatt ctaagagatt tggagaagag ttttctaaaa aattgttaga
    12601 agatgatgta gatgctgtaa ttttaacttc tacatgaggt acttgtactc gttgtggagc
    12661 aacgatggta aaagagattg agagagctgg tatagcagtt gttcatatat gtacagtagt
    12721 accaatatca cttacaatag gagctaatag aattgttcca gcagtaggga taccatatcc
    12781 acttggaaat cctatgttag gagaagagga aagtaaaaaa attagaaaga atatagtttt
    12841 aaaagcttta aatgcactaa cagaagatat agaagaacaa acagtatttg aataataaaa
    12901 atacaacaca acactgaaat aaaaaaagat atcaaaaaat aattttttaa aattatttta
    12961 cgatatcttt ttttattagg taaattcgaa agttgcatat tatttgcata tttttaaatt
    13021 aaatttataa aagtgataaa ttagtttatt ataatattga tacacaaatt caaattagat
    13081 atactatgaa aagtgagatt attacatttt ggaacaaata ttcaacatat taaaccagat
    13141 taattgaaca gaaaaaatta actttatata ataattagaa taatgataag aaagaaggtg
    13201 tatatattga tttcaattgc catttgtgat gatgaatcct atatgctaaa tcatttaaag
    13261 gaaaatatag aattatacat gaagcagaag aatttattat ataatataga gctttttaat
    13321 aaagtagaag atttactatg caatcatgaa aagtttaata ttgtattttt agatatacag
    13381 atggaagaga ttaacggaat gcaggcagct agaaaattaa ggtcatatgg taatgagtgc
    13441 tttataattt tcgttacagt tttaaatgaa tttgtatttg atgcttttga agtagatgct
    13501 ataaattatt tgataaaacc tctatctaat gagaaactat ataaaacttt ggataaaata
    13561 gttacaaaac tcgaggataa tgaaagtaat tatatcacaa tacaaaaagg ttattcatat
    13621 tggaaattaa aacttgctga tattttatat tgtgaagtta taaaaagaaa gatttatgtt
    13681 catttaaaga aagagactgt agattattat gagactattg aaaatttaga gagaagttta
    13741 ccaacaaatt tttttaggtg tcatcgtagt tatataatca atttacaata tgtatatgga
    13801 tatgaaaatg gtaatgcagt tatggaaagt ggagatagaa tacctgtttc tagactacgt
    13861 caacaaatat tttcaaaagt tatgcttgag ttcatgaaaa ataggaggat ataagatgat
    13921 taatttattt tcttccgatt tatttttaaa gtacagtttt ttattttctt ttattgtata
    13981 tagaatttta atggtaatag tgcaatacta ttttctaatc agttttacaa atgttaattc
    14041 aaaattttgg tgttttattt tatattcgat aatttcaatt tttttaatca ttataaatac
    14101 atattttaat ttatataatt tattttcttc acagattcta ttttttctta tgactttttt
    14161 atttgtaaag tacataataa aacaatctac tgttttatct atagtatcaa ctttgttagt
    14221 atatttaatt gaacaattag tatatggatt tacagctcca ttaaattact tgatatttac
    14281 tagtgatata aatacagata tttataaatt tgatataaat atgggactat cttctttaat
    14341 tactattata atagctggat ttatatattg gtatagttct aaaaagttta atattaaggt
    14401 catgaacttt gataaatata tagcaatttt gatgattcca ttgttgctta taatattatt
    14461 tatgcaatca tttgaatata gttcaaatat aaatatagat acaagtcttg ggattgtaaa
    14521 gctgctatta aatacttcta taactgaaga aattcaagct tttctttttt ctataattgg
    14581 aactatatgt gtattttttt ctctatttac ctttaagaaa ctaattcaag ctctcgaaga
    14641 tgacaaggag agggcaatta tgaatcaaca gattcatgct caaaaaaact atattgaaga
    14701 agcgaaatca cgattatcac aaactatatc ctttagacat gattttaata atcatttagc
    14761 tattgtcaat ggattgttga aaaaagacca aattttaaaa gcacaagatt atttaaataa
    14821 gcttgaaaaa gtaacaagtg acctatcatt ttcttgtaat acacagaata ttgttattga
    14881 tgctcttttc aacaataaat taagtatagc aaaacaatta ggcatagagg tagattgtga
    14941 agtaattata ccccaaagca ctaacatgaa cgattttgac ctatgtatag tgtttgctaa
    15001 tgctattgac aatgcaatta aagcttgtag agttgtagat aaacaaaata ggtatttaaa
    15061 gctttccact aagttagaag gtggattttt tatgatagaa ataaaaaata gctataatcc
    15121 aataaataat tatacacgtg gttcaggtat aggtctttta aatataaaag aaattgctaa
    15181 aaaatatcaa ggggctataa gtatagataa gacatcagat tattttcaaa taaatgtatt
    15241 gctggttatt tcattacatt gcgctagtat ttgagaacaa tttcattgaa aatagtattt
    15301 gtaagctata ttatgtattt gaatagagaa agtttgatat atagaaatga ggaggttact
    15361 aaaatgaaag ttggaaatat aaatggtcaa gcaccaatta caaatggttt agatagttgt
    15421 caaagtgagg tacaaggctt agaacaaagg aaaaaatcat tacaggatga aatagagaaa
    15481 gtaagaagtg ataaaagtat tgatggtgaa acagccaaat taaaaataga agctttaaaa
    15541 aaagaaatgg aagagattga aaataaaatt caagagttaa aaggaaataa agcaaaaaaa
    15601 acaaataaag aagaaaataa tgtttcagaa gtaaataata atctattaga gagaaaaaaa
    15661 gaaccttcta ataatgaaaa tgtaaaacaa ctatatgatg tttttgaaag aagcaatgta
    15721 gacgaacaat gggataatac ttatcgtgtt ctccaagatg ataaaaactt aaaagtaaaa
    15781 tttaatagac ctatatagtt tatttataaa aatagttaat attttaatta tcaccacttt
    15841 ttagaagaat acacacttta ttttaaagac ccacatattt atttacagtt agcacattta
    15901 tatgtggttt tataactatt atttacaagt ctatattgat aacttttaca tagtatataa
    15961 tgtttaaata cttattcaat tttatctata cataaacaaa aaaagagatt ttaaaatctc
    16021 tttttagatt gttgacttat ggctttttga tttaataaaa tcaagaagcc gtttgaattt
    16081 tatatgttgg ttgataccct tctgatattg aaacaccatt tttgtttcca aaatcgttta
    16141 aaattaagaa cttattatga atgactattt cattatgaac aataagcaga taacatgaat
    16201 cttcaatcaa tgttttaaca gtagcattaa atgcttgctt atacagttct atcgtttgtt
    16261 tgaactgtcc attaaagtaa atatgagtca ataacataac tatatctaca taaattaata
    16321 taaatttttt atactgtaca ttatctaact gttgattatc tgtattacaa catgattgaa
    16381 tttaaaaagc catcaacaca tagaatgttg atggcctgaa aatagattta aaatctcttt
    16441 tttattatat attaattatt ttttaaattt atattttttg catcagattt tctaatagct
    16501 aagtcaaacc ctctaaatct aatcactata gggtcattaa gaggagctat ctttttcact
    16561 tcaacttcag tgccatctat tagtcctaag gctagaagtc tcttcataag tttttcattt
    16621 ccagctatat tgtctataat tcccttttgt ccaagtttta agttgtaaac agtcattatt
    16681 aacacctcct aatgaaaatc attatcaaac tttatattta taatatataa caaaaagagc
    16741 aaattgtcaa tatataacaa aaaataaaca tactaagtta gtatgtttat aaaaaatagt
    16801 atacatggga tactaatagc atctagcaaa aaaccactta ctaatggtac aaccaaaaag
    16861 gcttttggag aatcaccata tttttgtgtc aaagaactca tatttgccaa agcgttcgga
    16921 gtagctccta ttccatgtcc aatcaaacca cttattatga tggcagaatc aaaatctttt
    16981 ccaagtactc taaagcaaat aaatattgaa aataggatta taaagattac ttgaaaagtt
    17041 actattaaga gaataggagg taataatgta gataattcaa ataaatctat attcataagt
    17101 gataaagtta aaaaaattcc caatgaaagt tcttttaaaa aatctataag tttaaaattt
    17161 aattctagta tatttatctt atcatttaaa tttctaaaaa taacagccga aaacatacaa
    17221 gttacaacag agggtaatag tatattaaac gttatataga agcatctact tataatttct
    17281 cctgtattta tacaaataaa tatgagaaga acctgttcca aaaatacaga tggtgttatt
    17341 gtttcggaga agttattatt ttttatccta ttcttatttc taaaaaattt acttccaaat
    17401 ctctttagat ttctattata tctaggaatc gacaagtcta tatttgatga tggttttaat
    17461 ttatatttat ttattagaaa tttagctaca ggacatccca atataccacc cataataagt
    17521 cctattgttg cagcagataa tccaacccca actgctcctt ctataccgag attttctatt
    17581 gtaagtccaa atgcagcaga ataaccatga ccaccttcca tagatacatt tccacacata
    17641 agccctaaaa gaggttcaag ttttataatc tttgatattg taatagctaa tatattttga
    17701 caaaatgcca atactccaca caaaatccaa tatctaaata atacactacc ccctttcttt
    17761 ataagattta tgcttatttc gagtcctata gttgtaaaaa agaaagttat aaaatttggc
    17821 attaaatttc cacttataga tatatctgct atattaaaat atttaaaaaa taaatttaga
    17881 aaacaaaata atagacctcc tattactggt gcaggtatac agaaattatt taaaaagtta
    17941 actttattat tcaataaatt accaattaaa aagaagaata tagatagtgt caaggtttgc
    18001 actatatcaa aacttaaggt aatcaaaata agtcctcctt tgtaattcaa agttttaatt
    18061 atataactta aattcttatg tattgaaatt ttattatcaa atgtatatta ataaatgtat
    18121 attaaaaaga atcagtagat ataattagat agtcataaat taaattaaaa tattttaatg
    18181 agatatgact tttttagaat taaaaaccgt agtatattga tataatagaa ataagactta
    18241 aaaatctgtt gatagtatta tttataagta cagtaggagg ataaaatgtt tgaaaattta
    18301 cctaagatag atttacattg ccacttagat ggaagtgtta gagtagaaac catgcttgat
    18361 atagctatta aagagaaaat agatttacct tcaaataata tggatgaaat aaaaaaatta
    18421 gcaaaggttt cttttaattg tacttcacta gatgaatatt tagaaaaatt tgatttaccc
    18481 ttaaaagtaa tgcaatctaa ggaaaattta aaaagaatta cttttgaact tttggaagat
    18541 gcaagtagag aaaatgtaaa atatattgaa attagatttg caccattact tcacacccaa
    18601 aaaggtatga gtgttaaaaa tataattgag ggtataattg aaggaattag agaagctgaa
    18661 agtatttatg atataaaagg caatttaata ttaggttgta tgagaacaat gacctcaaaa
    18721 gaggcattat tagtaattga agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta
    18781 gacttgtgtg gtccagagaa agagggattt tgtaaggaat ataaagatgt ttttaaatta
    18841 gcaagagaat atggatataa agttacaata catgcaggag aagctgcaag tggagaaaat
    18901 gtattggatg ctataaatat tttaaaggcg gataggattg gtcatggggt aaaaattaaa
    18961 gaccataaaa aggcgtataa tttagttaaa gataaaaaaa tattgcttga attatgtcca
    19021 actagtaatg tacaaactaa gacagtagat tcttatgagg tacatccatt ttatactttt
    19081 tataaagata atttacatgt aagtataaat actgataata gaactgtatc agatataaat
    19141 ttaagttcag aactaaatgt tatatttgat acttttaaac taggattaga agactataaa
    19201 ataatttata gaaatgcagt agaagctagt tttgcagata aagaaactaa agagtatttg
    19261 aatagtttaa tttaatagat aaaaatattt aaactttaaa ttaagctatt aaattgacaa
    19321 aaatacattg tattaggaga aaaaatgtga aagattatga agatgttgtt tatattaaac
    19381 tagtcaaaaa agaacttgaa gaatggaaga aaaatatatt aaagaaacct tctatatttg
    19441 acaaagcatc aaagggtata cagaataaat ttaatggagt attgccagat aaatatcatg
    19501 aaataattac ttcttctata aaagtaatga caaaaaccgt actgtttgga tataaatatg
    19561 ctacaaagcc accacttaga aatatgtcat tagaagaaag agaaaaatta gttttagaaa
    19621 aagctaaaac ttacaagact acagcaatga ttgaaggggc aggaacaggg gcaggaggaa
    19681 tcctcttagg tttatctgat tttcctttgc ttttaggtat taaagtgaaa tttttatatg
    19741 atgtagcttc aatatatgga tatgatgtta aagattatag agaaaggatt tatatattaa
    19801 atatattaca gttggctttt tctagtcaac agcatataaa caaaactttt aaattgatga
    19861 ataactggga tgaatatgta aatagtctac cagaagacat caatgatttt gattggagaa
    19921 catttcaaca agagtatagg gattatttag atttagctaa gtttttacaa ctaatgcctg
    19981 gaataggagc atttgtagga ttttatgtga atggaaaatt aattgataaa ttatgtgaaa
    20041 ctgctgtgtc tgcttataga ttgaggattc gagagcttaa gttataagaa aaattataac
    20101 ataaaaaatt agttatacaa tatatctata tgtatctaat tttgcattga taaaaacaat
    20161 tatggataaa aggtaaagca tgatagaatc taatgtagag gtttgtattt taatggaaaa
    20221 atttagtttt tattgcttaa gatacaaatt gaattacgaa gagattgaat tatgaagaga
    20281 agtaattggg aggaatattt tcatgacaaa tttgccaaaa caatttgata gcttcaatga
    20341 agctagacaa aaaggattta taaatgcaaa ggaacttaag gaaagtggga aaaagatggt
    20401 tggagttttc tgtacattta ctccagttga aattccaatg gcagctggag caactgtagt
    20461 aggtgtatgc ggtgttagtg aagagccaat acctgatgca gaaagggtgc ttccaagaaa
    20521 tctttgtcct ttaattaaat caagctatgg acatgcaata acagatacct gtccttattt
    20581 ctatttttct gatttgttga taggagaaac cacttgtgat ggtaagaaaa aaatgtatga
    20641 agaacttgca aaagttaagc caacatatgt aatgcatttg ccaaatactt caaaaggaga
    20701 atttgcctat aaactttgga aagatgaaat gataagatta aaagaagagg ttgagaaatc
    20761 tttaggtgta actataactg aagaagatat aagaactgct ataaaagata aaaatgaaga
    20821 aagagaactt ttaaaagagt tttatgcact tggtaaatta caaccatctg cacttacagg
    20881 ccttgagtta cataatgttt tatatcaagc aggttttaaa tttgaccgtg cagaacttaa
    20941 acattcttta agaaaagtta tagatgatat gaaagaaaga tatgaaaaag gtgaatgtcc
    21001 tgttcaaaaa gataagccaa gaatattgat aacaggttct cctattggtg gaataagcga
    21061 aaaaatagtt aaaactttag aagatgcagg ggcatcagtc gttgcatatg aactttgtgg
    21121 agctattaga tcaaacgatt tattggttga tgaagaaata gaagatgtat atgatgcttt
    21181 aacacaaaaa tatataaata taggttgttc ttgtatgatg aataatgaca atcgtattga
    21241 attattagat agaataatag atgaatataa tgtagatgct gtaatagatg tagttttaca
    21301 agcatgtcat acatttaata tagaaagtta tagaataaga gaatttgtta caaaagaaaa
    21361 aaataagcca tttatgtctc ttgaaactga ttattcaaaa tcagatactg aacaattaag
    21421 aactagattt gaagcgtttg tagagatgtt gtaggaataa ataaaagtag gaggaactat
    21481 gtttagtata ggagttgact cagggtcagt ggcaacaaag ggtgtgctat ttgatggtga
    21541 aaaaattatt aagaaaataa ttataccaac tggatggagc cctaaaagta catctaaaca
    21601 ggtctatgag ttgctaagta gtgaaattga caaaaaagat ataaaaaagg tggtaggtac
    21661 aggttatgga aggggagtaa tggattttgc tgataagaaa gtaacagaaa taacttgtca
    21721 tactagaggt atttactttt taaacaataa cataaggact attttagatg taggtggtca
    21781 agatagtaaa gttataaatt tagatagaga tggaaatgta tttaacttta taatgaatga
    21841 taagtgtgct gcaggaactg gtcgattttt agagattact tctaaccttt taggaagcga
    21901 tattgaaagt atagatacat tagctaaagg atatgaacca gttaatatat ctagtatgtg
    21961 tacagttttt gctgagtctg aaattgtaag tcttctagct caaaacattt ctactggaga
    22021 ggtagcagca gggatactta aatctattgc aaataaatcc acctcaatgt tagcaagagg
    22081 tgaggtaata gatgaagttg catttactgg agggcttgca aaaagtaaag aactagtaaa
    22141 aatgatagaa gaaatactgg gtaaaaaaat ttttattgca gaagatactc aaataatagg
    22201 agctttagga gctgctgtaa taggatttag ataaaaacat ataaaaagaa aatgagaagc
    22261 tgaatttatt ataaataaat tcagtttttt attttatttt atttatttat aaaggaaaac
    22321 tttggtataa ttgatttaaa tgattgtata tttgtaaata cttgatattt agtcatatat
    22381 aacattataa ttttaaaaat tagctcaaaa taagggggag aaagcgtgaa aaaatttact
    22441 tcaaaaaaag taacaaggtt tgttgcgctg tttttaatat ctattttagt catgtcaaca
    22501 attcctgtat cagctgacaa tagcaataca ttgaatcaga taaaaaaagc agaatacagt
    22561 gagacgtata aacaatatct aaatgatgct aaaaatggac gtacagaaaa atataatggg
    22621 attataccaa atccatatca gttggaaggt actcaaatcc aaagtaaagg aagaacagct
    22681 ccaacatctt atgaccctag aaaattaggt cttatgacat caataaaaaa tcaagaggat
    22741 ttaggaatct gttgggattt tgctgcaatg gcaaccttag aaagtttctt aaagttaaat
    22801 aattatggag actatgattt atcagaagaa cacttaagat ggtgggcttc agatggtgaa
    22861 tatggatggt ctgtaaatga tatgaatggt gctttaaatt atgaagctat gggatattta
    22921 acatcttggt caggtcctaa attagaaaaa gatatcccat acaatggtag agtatctaaa
    22981 gcacaaggag caaaaaaacc aactaatatg aatacagcac ctactgtttt taatgtgaca
    23041 gatgctgtgt gtgtaagcaa tgacataaat tctacaaaaa atgctatatt acagtatgga
    23101 gctgtaacat ctggttatta tgaagatatt aaatatcaaa gtgatgacca aaattcatat
    23161 tattgtccaa caaaaagttt taatacaaat catgctattt caattgtagg ttgggatgat
    23221 aactattcta aagataattt taattcaaat attagaccat caaaaaatgg tgcttggtta
    23281 ataaaaaata gctggggaga ttataattct gagggtggat atttctggat atcttatgaa
    23341 gacaagacac ttatgtctga tatagataat ttttcaatca aaggtggtaa aaaaccaaat
    23401 gatgatgaga agatgtatca acatgactat gcaagtattg tacctttaat ttcaaaaaag
    23461 ataacagctg caaatgtatt tgatttcaat agaggcgatg aaactttaaa atctgtaatg
    23521 ttcttaactg aaagtatagg agctaagtat gaagtatatt atgctccagt agtaaatggt
    23581 atacctcaag aaaacaatat gaaaaaatta aaagaaggta ctgcccaata ttcaggatac
    23641 ataacagttc caattgattc ttttgatata ccagaaggaa agggtgcaat tgtagtcagt
    23701 atagagggga aaaatggaga gtcaactata ggttctgagt caaatgtacc aggatatgat
    23761 acatttaaag caaaagctaa tttaggtgaa agttatatca tagataatac tggaaaattc
    23821 tttgatataa atagagatag taacttctat ccgtgtaatt ttactataaa agctgttaca
    23881 gaaaagtctt ctggtgagag tatcccaaat gagtctttaa ttggtagtga tagatatgaa
    23941 actgctatca aagttagtca aaatggtttc aattcttcag aaaatgtagt tttagtaaat
    24001 ggcagttcaa tagtagatgc attggcagca actcctttta catctgctat aaattcacca
    24061 atattattga cacaaaaaga agctttaaat tcaaaaacta aagctgagat acagagatta
    24121 ggtgctaaga aagtatacct aataggtgga gaaaattcta taagtaaaga aattgaacaa
    24181 caattaaaaa gcttaaatat atcgatagag agaatatcag gaagtgatag atacaagaca
    24241 agtttattat tagctcaaaa gttaaatggt ataaaaaatg tatctcaaat agcagttgta
    24301 aatggagtga aaggattagc tgatgcaatt agtgtaggcg ctgcagcagc agaaaataac
    24361 ataccaatca tacttgcaaa tgaaaagagc gaattgcaag gagctgatga atttctaaat
    24421 tcattaaata tagagaaatc ttatattatt ggaggaacag catccctatc aaacaatcta
    24481 gaaagtaagt taaaaaatcc aactagatta tctggtagta gtagagacga aactaattca
    24541 aagataatag ataattttta taaaaaagat actttaaaaa atgcatttgt agttaaaaat
    24601 ggaatcaaga atcaaaatga tttaatagat ggattggcag ttggtccttt aggagcaaaa
    24661 actgggtcac cagtaatttt agttggtgat aaattagctg atagccaaaa agaagttctt
    24721 aaaaataaga ctttagaaaa agtaactcaa gttggtggag gagcaaataa aaatgcattt
    24781 aaagagttag taaaattaaa aagtgctaaa taaggtactt aagtattaac atatactagt
    24841 gtctattagt aatttatctt aataaaagct gaaaataatt actttaaatt attgataaat
    24901 aagataatca atgattgtaa aattttattt tcagctttaa atttatgtag tataagttta
    24961 aaaacataaa aaagggttac tttatataga aatatagaaa atcttacatt ttatatataa
    25021 ataacccata tttaattaac tttttatagt gttctaatag atattattga acttaatggt
    25081 aaatataatg tgtaagtttc atttaaagtt ggattagatt catctatata tcttgagcaa
    25141 agtgtaattg ataagctatc tgcttctata atttcaccat atagaggaca actgctaaag
    25201 taagaatgta cttcaacata tttatttaag ttttcttgaa cataatcgta aaaacggttt
    25261 tttgtagtag tagccataaa atcaactcct ttatttttta ttaaagttga aacttttaaa
    25321 aagataaata tgattacttc tattagaaac taagttaaaa aataagcaaa tagatatgaa
    25381 aattagaata atatattatt tatatcttaa ttataacact agataataaa aaatgctata
    25441 taaaattcag aattttcgaa agtttttaag cttattttta aatatactaa taatactatt
    25501 gacattagat agaaaaagta ttatattgac tatataaatt attttagtca aactggagga
    25561 aaaagatgcc taaatcatat tctgataaag aaagagagta tataataaaa agacttaaag
    25621 aagaagcaag attgtgtatg gagcaatatg gtattagaaa aactactgtt gatgagcttg
    25681 taaaacgggt aaaaatacca aaaggaactt tttatctatt ttttcaatca aaagaattgc
    25741 tcttttttga ggtattaaga gatatacatg actctataca aaaagaaatc ttatatgaga
    25801 taaatagagt agatgaaagt attacatgtg aacaattgac agatatattt atgaaattct
    25861 ataggatggt tgatagtact tcaattttaa atctcatgat aaatggagaa tttgagatat
    25921 tggtgagaaa gttaccagat tctataattg aagaacactt tagacatgat gactttgaaa
    25981 ttggagagat tatatcttgc ataccaaatg caaaaaataa agacatagaa agctttagtg
    26041 gagcatttag agcagttttt cttaccatgc tatataagcg cgaggttgga agtaattgtt
    26101 ttgaagatgc tttaagatta atgataaacg gattagttat acaacttatg gaatagaatt
    26161 taaattttgt ttgctaataa cttttattaa aaattattaa caaacaaaaa aattatattt
    26221 tattgatcat atagtcaaaa tagtcaaaaa tttgaaaggg tgatattgaa tgattaaagt
    26281 tgatgattta tcatttagtt atacagatag agatttttta caaaatatta actttggagt
    26341 aggaaaaggg gaaatactag gatttttagg accatcaggt gcaggaaaat ctacattaca
    26401 aaagattcta attggaatga taactaatta tggaggaagt gtcattgtaa atggagtaga
    26461 gagtaaaaga cactcaaata aattttatga aaatatagga gttgattttg aatttcctag
    26521 tttatatgaa aagttgactg caatagaaaa tttaaaatat tttgggtctc tatattctaa
    26581 aaaactacta tctattgatg agttattaaa atcagtagga ctagaaaatg aagcaaataa
    26641 aagggtatct gagtattcaa aaggtatgaa atctcgatta aattttataa aagctttatt
    26701 acataatcca gatattttat ttttagatga accaacaagt ggtcttgacc catcaaatag
    26761 caaggttatg aaagatataa ttttatcaga aaaattgaaa ggtaaaacta ttattttaac
    26821 aactcataac atgttagatg caacagagct ttgtgacaga gtagcgttca ttgtaaatgg
    26881 taaaatatct gctttagata caccacataa tcttattatg tcaaaaggag ctattaaagt
    26941 tagatatact tactttgata atggagaaaa aactgctgaa tgttttctga acaatactgc
    27001 taatgataaa aatttgaata tgcttattga gaaaaataaa cttttatcaa tacatagtag
    27061 tgaaccaaca ttaaatgata tttttattga gataacaggg aggaatttac aatgaggcta
    27121 aaaaatctta ttttaggaga tataaagttt caatttaagt atggttttta ttttttatat
    27181 ctttttttaa gtattatata tatttgtatt atcaatgtat ttccaacttt tatgagagaa
    27241 aaaatagtta taattatgat ttattctgac ccagcagcaa tgggattgtt ttttatggga
    27301 gctattgtac ttcttgagaa aagtcaaagg gttttaaatt ccttagctgt atctccagta
    27361 aaagtatctg agtatatttt atcaaaggta atttcattag gagtaatatc tagtattgta
    27421 gctatgttta ttgctattac attaaattta gataatatta taatatcaac tataggtacc
    27481 tttttttctt ctattatatt ttctttgctt ggattgatac ttgcaagtaa agcatctagt
    27541 ttgaatcagt ttattgtttt aagtattcct attgagatta tatgctttat tccaccaata
    27601 ctcaatgtac ttcttgacac aaaatcatat gcaaatttgt acccttttaa tatatgtatt
    27661 tctcttatta gtggagataa aaattttata atgattaata ttttaatttt aatatcaatt
    27721 ataataataa tttattttat aacttattat tttatttgta gcagttggaa aaaggtaggt
    27781 ggagtaaaac tatgataaag attataaatg cgtttaaaca agagtttgaa caaattaaga
    27841 gagatgctat gctttttata gtatgtgttt ctccaatatt atgtggagta tttataaaat
    27901 ttggaatacc attaatacag aatatatcac ttaacaagtt ttattatcaa ttaaatttag
    27961 agccgtattt cttgatgttt gatttacttt tagcttttat tacacctttt atgttctttt
    28021 ttgcttctac aatggtcata ttgggagaaa ttgatgacag catttcaaga tatttaatag
    28081 taactccgct tggtaaaact ggttatctta tttcacgatt tggaatacca ggtatcttag
    28141 catttattat tactatgatt ctacttatct ttttttctct aactaaaatt tctttcttgt
    28201 taaacttatc aatttcttta ttagttttat tacaaggaat tattatatca atattagtga
    28261 tttctttatc atcaaataaa ctagaaggaa tggtaattac gaaattctca ggtgttttta
    28321 tgctgggtat tttagcacca ttttttattt taaataaagc acaatatatt ctgttcttct
    28381 taccaacttt ttggatatcg aaggcattta aggaagataa ttatatctat atgtttattt
    28441 caattgttat atcattaata tggattttat tattatttaa aaagtttagt aaaaaaatag
    28501 ttaaataaga aaatttaata tataaatgta aggttgactt gacagtaatg ttgaaaaatg
    28561 ttacatttaa gatgtaaagt taaccttaca tgaatggagc aaatatgaaa aataaaataa
    28621 agatattaag agaaaaatta ggattgacac aagaacaact agggagatta gttggaacat
    28681 ctagacaggc tataaatgct atagaaactg gtaaaaatga accttcaata tggttggcat
    28741 atgatatttc aagaattttt aatgaaccaa ttgaaagtgt atttttattt gaagaaagtg
    28801 aaagaaaatc aagagcacag ataagtaggg gagaatatta tggctataag aaagataaga
    28861 acatttgatg atgaaatttt aagaaaaaag agtaagtatg tagaaaacgt agataataag
    28921 ataagagaaa tattaaatga catggcagag accatgtaca atactccaaa tggaggagga
    28981 ttagctgcat gccaagtagg agtattaaag cgattagttg ttatagattt aggagaagga
    29041 cttattaagc ttgttaatcc tgaaattatt aaacaagaag gggaacaaat tgttgttgaa
    29101 ggatgtttaa gttttcctga agtatggggt aaattaaaaa gaccaaaaaa agttacagta
    29161 caagctctaa acgaatacgg tgagaaaata gaaattaaag gttcaggttt tatggcaaaa
    29221 tgtctttgtc atgagataga ccatttaaat ggtatagttt ttacagataa aattattgaa
    29281 catgtaaaat tgtaagatag attggttatt gaatttataa aatattttat agtaaggagt
    29341 taaattatga gattatggca taaggattta attgatgtat tacctaaaaa tcaattagta
    29401 agtcaatgga gagagctact tgctataaaa ggttctatag ataaaaaagg tactccaaac
    29461 catctattag tgaataaagt attgaattat agtatagatg aatttaaatt ttatacaaaa
    29521 attgtacatg atgaaatgtt aaaaagaaat tataaaccta atgaacttaa atatacaagt
    29581 atattaaaat ggaaaaatag aaattttgca aatgatatat ctaatgaaca tagtttaaat
    29641 ttagaaaatt tatatgatga ttggcataat aaaatgtatt taaagcaatg cttatataat
    29701 ttagaagaaa aagcaacatg tggaggaata ccaataaatg aatggaatat attactttgt
    29761 aaatatagta aagattatga attgtggtct ggaaatatca tgttttaatt actatagatt
    29821 aacattatag tattattgta tataaagagg tgattatttg tataataaaa taaaatatat
    29881 tccaataaat tgtgaaatag cttgtaataa attaaaaagg agtatgccat ataaatggga
    29941 tttaaatatt tatcgtggat gtgagcatgg atgtaaatat tgttatgcta tttactctca
    30001 taagtacatc aactccaata attattttga agagatttat gtaaaaacaa atatagtaga
    30061 aaaacttgag agacaactta aaagcagtaa atggaagaaa gaagtaatca atataggagg
    30121 agttacagat agttatcaac caatagaagc tgactataaa attatgccag aaatattaaa
    30181 cctgctgata aaatacaaga caccagctat tatttctaca aagtcagatt taattttaag
    30241 agattacgat ttaattgata agctatctag gattacatat atcaatgtag catctacaat
    30301 taccacagta aatgaagata cacaaaggct tattgagcca aatggtgtgg attcaatgag
    30361 acgatttaaa atgttaaaag aatttagaaa aactaacgca tcagttggtc tacatattat
    30421 gccaattata ccatatatta cagatgattt tgataatata aattcgctat ttagacatgc
    30481 aaaagaatgt aatgtacatt atgttttacc tgggacttta tacttaaggg gtataactag
    30541 aggtgttttt tttgactttg ttaaaaaaga gtttcctgaa ttatttgata aattatcaat
    30601 actatatagt gcaggttcac ttaataggga gtataaaaat caattgtata aaatggttaa
    30661 tgaacttaga tataagtatt ctctatcaag cagttactct aaaataatga aggaaaagtt
    30721 aaaaaactca ggagatgtac agatatcttt ttttgattag ttttatataa aaaaaatttt
    30781 tttactatga tgattaaaat ttattaaaat taaaatttat tgacaaatat aaaatttaaa
    30841 tatataatta attcataaaa taaaaatatg aaaggatgat gttgttcggt gatatttgat
    30901 atcataaaat cgttgatata agtattgctg gaaaagagtg aataaaatca aatttaataa
    30961 gcaaacaaag taaagctata gttttattgt tgtgcttaaa aatatttggt tctattttac
    31021 tcttatttaa actcatcttt ttgaagttaa accacagagt aaactctgtg gtttttttat
    31081 tttaaaaata aattttaagg agtggtatgt tttgttaaac aaattatgta tgtatttaga
    31141 aagaccagag ctttataaac aaagtgaaat taatttttgg gatgatgagt atatttcaaa
    31201 gcaattatta aaagcacact tagacactaa ttttgaagga gcaagtagaa attttaactt
    31261 tatcgaggat tctgtaaatt ggatagttac agttgcaaat ccagctaatt atcctaaatt
    31321 acttgattta ggttgtggtc caggtttata tgcagagaaa tttgcacaaa aaggttataa
    31381 ggtaactgga attgatttct caaaacgttc tattaactat gcacaaaata gaaataagga
    31441 gactaattta aacataaatt acttgtttca aagttattta aatatgaact ataatgagga
    31501 atttgatttg gctaccttaa tttattgtga ttatggagct ttgtcaactg aaaatagaag
    31561 gttattaatg gaaaaaattt atgatagttt aaaacctgga ggaaaattaa tattagatgt
    31621 atttacaata aataaataca ataattttga ggaaattaaa tattgggaaa taaatgaaga
    31681 tggaggattt tggagtaatg aaaaatatat gtgtctacaa gataattgta aatacaatga
    31741 ttataataca ttagaacaaa ctcttgtaat aaccgaaaaa gatgaaaatg tattttatgt
    31801 ttggaatcac tatttttcaa aagaaagttt cttattagaa gttgaaaata ttggatttaa
    31861 gtctgttgag ttttttagta atgttaaggg tgaagcatat tctgacgata gtatgactat
    31921 gggattagtt ttacaaaaat aataattttt ttaaaatgaa taaataaaaa cttgaaacct
    31981 ttaacaataa aataacataa taaaagctta catattgaaa aatggaaact tttatgttat
    32041 ttttagtaca ttacaaattt gcttatataa aaatcaatac ttgaattatt atttttatgt
    32101 gaaatttatc tatagtatat ttttataaac aagtaatgtt attaaaatta agtatatagt
    32161 taaaagtgta tagcaaaaag gctgttatat taatgttaga ggttcaggga aaatatataa
    32221 agcaaagtat ttacagataa tgtagattgc acagctataa gttagataat agaattatgt
    32281 aatcaagaat ttacaacaaa gtataagata aggataatgc taaattgcca tacaggtgct
    32341 agatgtgtaa atggatttaa ttaatactat tgataaagtg caacaagcac ataaaccaat
    32401 tgatgagatt attaaaacta taagagatat agttgacata attaaaatat tggaacctat
    32461 ttataatttt aaatcaaatc aataatgatt aagattaaaa taatattaag gcatcaatgt
    32521 tataagagta caatataagg agtactatat agtactcctt acttatacat attaggcaat
    32581 aggaaatttt tcatagttaa attttcaaat attttttagg gaactttata attgagaatt
    32641 aagggtatag tatgtgatga acttactaaa tttataatta ggttatataa tggaataatc
    32701 atcaatagtt ttactttagg gaaaatatat gcttaaggaa gtatagctta ctaatattat
    32761 taaaatgtat atttaaaatg ataaaaaaag aattagacaa gtatttagta aattgactat
    32821 gcaaaattct atcaagcagg aacactatga ctttagtcat gggaggttca gtaggtaact
    32881 tgttgaatta agtttatatt atttaaataa actacttaat ttagataata taaaaagtca
    32941 gaattttaag aatagagcta tataatctta gctattagga gaagtactat gataggaata
    33001 tattttagtg gaacaggaaa ttcaagatac tgtgtagaaa aatttttaca agaatatgat
    33061 ataacagcaa attcttattc tattgaagat aatgaacttt tacagcacat caataatcat
    33121 gagaatatta tttttagtta tccggtgcaa tatagtaatg tgccaaaaat attgaaagat
    33181 tttataataa atcattcaac tttgtggaat ggtaaacgtg tttttattat tgcaactatg
    33241 gctctattta gtggagatgg ctctggtgtg ttagctagaa tattaaaaaa atacggtgct
    33301 ataactgttg gtggattaca tgtgaagatg ccagatagta taggagatga aaaggtatta
    33361 aaacattctt tagagtataa tagaaagctt gtaataaaag cagaaaagaa agtaactaag
    33421 gctgctaaaa aactgaaaca tgggtcacca ccaaaagaag ggttgggttt tttgagtcat
    33481 attgtagggt tatttggaca acgattgtat tttatcaata aaacaaatat gtatacagat
    33541 aaactaaaaa ttaatactga ggaatgtatt gtatgtggta aatgcgttaa tttatgtcca
    33601 atgaagaatc tagtaattaa aaattgtatg attgttgcaa atgggcaatg tacaatgtgt
    33661 tatcgttgtg taaataattg tgccaaaaaa gcaattacat tgttaggtaa aaaagttgtt
    33721 gaacagagta atattaagaa atatatgtaa gaaatttaaa gttttattga atgtaaattg
    33781 atgtatattt tatgttagaa agagaggtta aataatggaa tactacatag ttgattcatt
    33841 tgcaactaaa ctatttaaag gaaatcctgc tggagtttgt gttcttgaca gaagaatacc
    33901 tttagagtta atgcagaaaa tagcagaaga aaacaattta ccagaaacag catttgtagt
    33961 taaaggtaaa ggaaattatg aattacgatg gtttactcct aaagcagaaa ttgatttatg
    34021 tggtcatgca actttagcag cagcttatgt aatatcaaat tttattgatg ttaatgtaaa
    34081 aaggatagat ttctttactc aaagtggaaa gcttgaagtt acacgaaatg gaaatctata
    34141 tgaaatgata tttccagaaa taatgcctat tgaaatagag ttatcaccac aacaagctaa
    34201 tttgataggc tgtgtaccaa gtgctgttta ttcttcaaga gatttaatct tattgctaaa
    34261 cagtgaacaa gaagttataa attataaacc taattacgca caattgcgta aattgacaga
    34321 ctggttaggt ataataatca cagcacaagg aagtaataca gattttgtat caagatattt
    34381 ttgtccagaa ttagattcag aagaccctgt tactggttca tcacattgta atcttattcc
    34441 atattggtct gaaaagttgg gaaaacacaa aatggtagct gctcaacttt ctaatcgtgg
    34501 tggtataatt cagtgtgaag tgttgaagga taatactgta aaaatttctg gtgaagcagt
    34561 cttgtttatg cagggaacaa taaagattga tatttaaata agtataataa ctaatttatt
    34621 atgaaaaata attaattttc ttagtgttaa caataataaa gtagagttaa aaaacttgat
    34681 ttcacttaaa tcaatatgat agatgttgat actaagattg acaagcatga ttaaaaaaga
    34741 taatatttaa tctataaaga taaaaacaca gtctggttgg tagtccagac gcagcaatag
    34801 ctgtcagtaa cctgcctcct tggttgtccg ttctttagaa agtaatttaa ggaggaacta
    34861 gcaatggaca aaattagtgg aaaattaaca gtcttatttg aagaaccatt ttgggtaggt
    34921 atatttgaaa gacaagatgg taaaaaatat gaagcatgta gggtagtttt tggagcagaa
    34981 cctaaagaag tagaagtgta tgaatttata cttgaaagat ttttctcatt agattttggt
    35041 agcattaaat tagaaaaaaa tgtgaccaaa gacaacattg gatacaaaag aatgcagaga
    35101 aaagtaaaaa aggaacaaga aaaagaaact atagggacta aggctcaaaa tgctcttaag
    35161 cttcaatatg aagaaagaaa acaggataga aaaaacctag ctaaaaatag aaaagaagaa
    35221 gaaaaagaaa gactatttaa tttgaaacaa gaaaagagaa aagctaaaca taagggtcat
    35281 taattttaat taaatatctt tttaataatt gctagaaaaa ggtgtataaa atttttaatt
    35341 ttatacacct taatttattg aaaagaataa ggttttatat ttcaattact ttaaaatata
    35401 aaaatatact aataaaaatt gtaaaaatag tataaatggt ataccaaatg taaatttaat
    35461 gtgtttagtt ttatgtctaa aagaatacat tccaattata gaacctatac tacctccaat
    35521 tactgctata gacataagag tagcttcttt tattctccat tcgtttctaa ttgctctttt
    35581 cttatctatg tacattgaaa agaaagcaat agaattaatc actataaaat atattagaat
    35641 aaaatttttc acagtcttgc ctccataaaa atatattata cattataaaa caagatattt
    35701 gttttggaaa gttaaatttg taattagtac atataaaata tttgataaga ataaatataa
    35761 tattaattgg aaaatttaaa aaaattaaac aattagcaaa tagggggtat atttataaga
    35821 cacaaagtga taaaatctat ttagaaaata atgtaaaata acaggaggat aaagcaaaat
    35881 gaaatttata gtagaaaaag aagtttttga taaattagaa aatgtatgct ttggtgtagt
    35941 agttgcaaaa ggaatagata atactaaaga aatagaaaga ataagcaacc ttttagatat
    36001 aagtatagat agagtagaag attacttcaa agataaaaaa gtaaaggaat cagaagaaat
    36061 tataccatat agagaggcat ttagaagtct aggcatgaac ccaaataaat ttatgagttc
    36121 gatagaagca atgacaacaa gggttgctaa gaataagaaa cttcctcata taaatcctat
    36181 tgtagattta ggaaactcta tttctttaaa atatttactt ccaatgggcg ctcatgatat
    36241 ggattttaga aatgatgatg tatatgttag attttctaaa aaaggggata aatttgtacc
    36301 atttggagaa actgatgttg aattaatgga agaaggagaa ttaatttatt cagttggaga
    36361 catggtaaaa actagaagat ggatatggag acaaggagag gaaggaaaaa ttactaatag
    36421 ctcaaaaaat atattttttc ctatagatgg ttttactgat gctaatttag ataaggtaat
    36481 gagtgcaaga gaagaattag caaaattgtt aaaggaaata tttaattgtg aaattaaagt
    36541 aggttttgta gataaagata atcctgaaat ggaaatataa taagttgttg aatacttgtt
    36601 aagatattat atttacttaa taatgttaaa aaggtattaa aaaagaaagg tcgtagtaaa
    36661 tttgaaaaaa actataataa agaatgtaaa tccaatgatt gaaaataagt ttataggact
    36721 ttttgaaata gagtataaaa acaaattaga tgaagataag gtttggatgg tagcatcaag
    36781 aaaaagtagt gagcaattaa aaaatattta tttgaagaat gaagaagata gtgtagatgc
    36841 agttgtaata gttgggcttc ataaaagtag taaaaaatta attttaataa gacaatttag
    36901 agtacctatt aatggatata tatacgagtt accagcaggt cttgttgatg aaggagagtc
    36961 tatagatatt tccgttgaaa gagaacttag agaagaaaca ggacttactc ttttagaaat
    37021 taaaaaaaat aaaagtattg ataaagttta tttatctcct ggaatgagtg atgaatctgt
    37081 tgcctttgtt tattgtatat gtgatggtga tataacagat gaatttttag aaccagatga
    37141 agagattgaa gcgcttttaa tttcgcaaga agatgcaaaa gagattttac aaagtaatca
    37201 taagatagac acaaaagcat ttttgatttt acaaatgttt gtgagtttag gagctaaatt
    37261 atttgaatag atatatagaa aagtaaaata ataaagttta ttttacttgt ataaattata
    37321 atctcttttt ttatacaata aagtataatt ttgtgtattt ttaaataagt gacttgactc
    37381 ttaaattttc ataatatgtt ataaaccaat ttaagatact acaataatta aaattccaaa
    37441 attatttaac atatttgcaa ttaaaatgag aaaaggtaaa ggctgaatga ttgaaaaatt
    37501 tgatgtaaaa tagtagatta gtttaatact atagtttgta gggttaaaaa ttagaaattg
    37561 taagggagat aaattctatg aaaaaattag ttattagttt aattagtatc attagtgtgg
    37621 ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata taataattta aacaacaaag
    37681 aatcaggaca aacaaccatt gaaatggact tagataaaaa ttatgatact tcagacccat
    37741 ttgttaatgc atgtttgttt tgtgtgtcta atgatataga tgttttagac acagagattt
    37801 cttttaaaat ggatggagat agtggtattg tggaaataaa ggacaataaa acagatgaaa
    37861 ctttatggag taatatatgg catggaagcg ttgataatga tacattcgca atttctcttt
    37921 ctaatataca aaaagagaaa gagtatgcta tatgctttac tggaattaaa ataaatcatg
    37981 cagttgtaaa ggttactttt gaaagcaatt tagttaagga gaaagaaaaa ccctcaaaat
    38041 aaattccaga ttgtctataa cttgatattc agtacataaa agcagttgat atttatgatt
    38101 gactgctttt tcttatcaaa aaggataggt tgtgcatttt tacttgaaat agtttatgca
    38161 catcaaaagt tggtgagcat gatattggaa atggtaagca tgaaatcttg ataacacaag
    38221 gaaataaatt taagttttca attaagtata tttccataat ttagttaaca ataagctaat
    38281 tattatttat attttgatat ataaataata attgtagcaa attttaaaaa atcatatatt
    38341 ttttgaacta aataaaaatt tatttttatt attttaaatt agaggagatt cttgaagata
    38401 tattattttt aaaaataagt atagtttaaa aattcaaaaa aaagatggca aaatatgtcc
    38461 caagggacat tgagtaaaat aaatgatttc agcttcttta cactaaaaag tacatactaa
    38521 atgaggggta aaataaagaa aaatgatata ctaattgtct tttgataata aaaaaacatg
    38581 gaatttgatt atttaatcaa gaaaaagtat tgaacaaatg atttatttag tgtacaataa
    38641 tcatgtaaaa acatagtaat ggagggagac atttcataat gaaaattaag gtaggaatga
    38701 acggatttgg aagaatagga agagctgtat taagaatagc tcaagaagaa ttaggagata
    38761 atatagaaat tgtagccata aatgcaagag caacaacaga atctttggca catttattta
    38821 catatgattc atgctatgga acttttagag gtgaagtaga ggcaaaggat gaagacactc
    38881 tagtagtaaa ctctaaagag ataaaaatat taagatataa tgacccagaa gaattacctt
    38941 ggaaagaatt gggggttgat atagtaatag aatcaacagg attatttaca caaagagaaa
    39001 aggcagaaaa acatattaaa gcaggagcaa aaaaagtaat tataacagct ccaggaaaaa
    39061 atgaagatat aactatagtt atcggagtta atgagaaaga atatgataat gaaaagcaca
    39121 atataatttc aaatgcatct tgtactacaa attgtctagc tccttttgct aaagtattgg
    39181 acgaaaaatt tggtatagta aaagggttaa tgactacagt acattcatat actaacgatc
    39241 aaagaatatt agataaaagc cataaagatt tgagaagagc aagagcagcg gctgaatcta
    39301 taatacctac cactacagga gcagcaaaag ctgtgtcaag agtactacca caattagaag
    39361 gaaaattaaa tggtttttca cttagagtac caacaccaac agtttcactt gttgatttag
    39421 tgtgcgaact taaagaaaac gttacatctg agcaagttaa ctctgtgtta aaagatgctg
    39481 ctgaaggaga attaaaaggt gttttaggat attgtgacaa gccacttgtt tctatagatt
    39541 ataaaggaga ttcaagatca tctattatag acgctctatc cacaatggtt atagaagaca
    39601 atatggttaa ggtagtttct tggtatgata atgaatgggg atattcttca agaactgttg
    39661 acttagtaaa atatgtagct gaaaaattaa aataatatat atttcttaaa gcaaatttta
    39721 aaaaaatttt ttaaaaaaaa tatataaaac taaaaaaaaa gattatgaaa aatattgaag
    39781 gaaaaatttt ttcataatct tttttctttc atgcaaaaag atacaagtta caacttatat
    39841 ttgcatatta ggtcgttaga gaagtatata tatatttagt aaggaatcat actttttata
    39901 aaatgaaaga taagatgtcg aaaacttgca taaatctgaa aaatttttat tattaaaata
    39961 ctgatttcca ttttaaaaat gataaaaaaa tgacaaataa agttgcaaat agtaatactt
    40021 ataagtataa ttaaaaaaga gaaaaggttt tgttaaactt tataacaaaa aaagaaggga
    40081 tgattcaaaa tggagaagtt aacagacagg agacaggtta aggcagtaag catggaaacc
    40141 ttcattttcc taatattact agtagttggt tttgggtatg ttggtagtat tatgggagca
    40201 ggaatgatgt tcaaagtaat aatgagtaca gcacatgctt tactattgga gacagttttc
    40261 ttaattatgg ctatggcagt tcttgcaggc gcattaagcg cactactttc agagtttgga
    40321 gtaatagcgt taattaataa aattttcgct gtatttatga agcctttata tggactacct
    40381 ggtgctagta tagcaggagc tataacaact tatttatcag ataatccagc tattatacct
    40441 tttgctaaag ataaaacatt cacacagtat tttaaacaat accaagttcc agcattatgt
    40501 aacttaggaa cagcctttgg tatgggactt atcttaacaa catttatgat ttctcaaggt
    40561 acagaatatg tacttccagc attaattggt aatttaggtg caattatagg tagtgtcata
    40621 agtgttagaa taatgttgac atttactaag aagttttata aatacaatcc ggaagaagat
    40681 aaagcaacag gaacattaga gaaaaaggat gaatttagag aaatcagaga aggaaatgta
    40741 tttcaaagag ctttagatgc aattcttgaa ggtggtaaaa tgggggtaga tatgggaatg
    40801 gctataattc ctggagtcct agtagtatgt acattggtga tgttattaac ttttggacca
    40861 tctacagacc ctgttacagg tcaagaagtt tatacaggag ctgcatatga aggaataaaa
    40921 ttacttccag ttattggaga taaacttgga tttatattag aaccattatt tggatttaca
    40981 tcaccagagg caatagcatt ccctataact tcattgggag ctgttggtgc agcaatgtct
    41041 ctagttccag agtttataaa gtcaggagct ataactccta atgatatagc agtatttaca
    41101 gctatgggta tgtgttggag tggatattta agtacacata ttggtatgat ggatgcatta
    41161 aatgctagac agttggcagg taaagctatt ctttctcata caataggtgg tttatgtgca
    41221 ggagcagctg ctcactttat atttacactc gtaggataaa tgtaatcagc taagataaaa
    41281 aataatatac aaaaaaatgg ttgttgatat gtaataagaa tttacatatc aacaaccatt
    41341 tttttctgac taaagataat ttataataca taaacattaa gtggatatta gagttaagtt
    41401 atatttatgt aaaaagtaac aaaaatctgt agaaatttat gacaaaattt agtacactaa
    41461 ctatatgttc ttatttaaaa tttaaggtta gaattaggaa ttacctagta gagtaagctt
    41521 tgattaataa aagaatcata aaataaaaat attagtcata aagattataa ataacttact
    41581 taaaataaag ccatagaact tacaaaaatt aaaaaagcat ataaatatgg aggggtacaa
    41641 tggatgaatt atttcatata ggaattgatg taggttctac aacggtgaag gttgtggtac
    41701 ttaataatag taataatata gttcataaag aatatagaag acattattct gatgtaaaaa
    41761 aatcagttaa agaagtacta aatggtatat atgaaaaatt gggagatata aatactacca
    41821 ttataataac tggttcaggt ggaattggaa tatcaaaaaa attaggtgta aaatttgttc
    41881 aggaagttat atctagtaca aaatcaatag agtattttca tcctgagact gatgttgtca
    41941 tagaacttgg gggtgaagat gcaaagataa catatctaag tggaggaata gaccaaagaa
    42001 tgaatggaat atgtgcaggt ggcactggag cattcataga ccagatggca tcacttttaa
    42061 agacagatgc cagtggatta aatgaacttg ctaaagggta taatgttata tatcccatag
    42121 cttcaagatg tggtgttttt gctaaaacag atattcaacc tcttataaat gatggagcca
    42181 atcagactga tatagcaatg agtattttca atgcagttgt cgtacaaact gtgagtgtcc
    42241 tttcttgtgg aagaaaaata gaaggaaatg ttgcgttttt gggtggtcca ttgtactttt
    42301 tatctgaact tagagaagca tttaaaagag tattggattt aaaagatgaa aacataatct
    42361 tcccagaaaa tgcacaactt tatattgcta ttggcgcagg attattatca gtagatgaag
    42421 ataatataca actaaaatct ctaatagaaa aattggatag cataaagaat attgaagatg
    42481 gagaagtaaa tttattagaa cctttattta aagataaaaa tgagtatgaa gaattttcaa
    42541 gaagacatga aaaagaaaaa ataaattatg tggacataaa ttcaataaaa agtaattgtt
    42601 atttaggaat tgatgcaggc tcaactacaa ctaaagcagc acttattgat gaggatggaa
    42661 gacttgtata ttcttattat aatagcaatg aaggaaatcc attaaaaaca accattaaag
    42721 ttataaatga agtatatgat atattaccaa aaaatattaa aatattgagt tcaactgtaa
    42781 ctggatatgg agaaggtctt attaaaaaag ctcttaaaat cgataacgga gagatagaga
    42841 ctatagcaca ttacaaagct gctaaatttt tcaataaaga tgtagatttt atacttgata
    42901 ttggtggtca agatatgaag tgcctaaaaa taaaagatgg agtaattgat aatataatat
    42961 taaatgaggc ttgttcatca ggttgtggct catttttaga gacatttgca agttctttat
    43021 ctatgacaat agaagaattt gcacacgaag gtatttattc tcaaaatcct gtagatttag
    43081 gttcaagatg tactgtattt atgaattcta gagtaaaaca agctcaaaaa gaaggtgcaa
    43141 gtgttggaga tatatcagca gggctatctt attcagtaat aaaaaatgct ttatttaaag
    43201 taataaagat tagagattta aatgagattg gtaataatat tgttgtacaa ggtggaacct
    43261 tttataatga tttggtgctt agaagctttg aaaaactaat tggaaaaaat gtaattagac
    43321 ctaatatttc aggaattatg ggagcttttg gttcagcact tatagctaaa gaaaaatatg
    43381 aaagtggata caaaacatcc ttgttatcta aggaagagtt gaataatatc aagctagaag
    43441 caagtgtaac tagatgtaaa ggttgttcaa atcattgtct tcttacaata aataaatttt
    43501 ctgataatga aatttttatt tcaggtaata gatgtgagaa gggtgaagct atttatggtg
    43561 ataaaaaaat aggattagaa aagaaaaaac caataaattt atttaagtac aaatacaata
    43621 gaatttttag atataaacca ttggaaaaag aagaagcaaa aaatggggaa atagggatac
    43681 caagagtatt aaatatgtat gaagattatc cattttggtt tacttttttc aatgctatgg
    43741 gctttagagt tgtactttca gataggtcat caaaacagtt atatgaaact ggtataacta
    43801 gtattgcatc tgaaacagtt tgttatccag gaaaattagt tcatggacat atagaaaatt
    43861 taattgcaaa aggtataaaa aatatatttt atccaagtgt aacaaatgaa aataaagagg
    43921 attcaaatgc agataattat tataattgtc ctgttgtaat atcatattct gaagtaataa
    43981 aaaacaatgt agaaaatata agaaataaga acataaatta tataaatcca tttataagtc
    44041 taaatgataa ggaaaaattg aagaaaagac tatatgatga attatcaaga catttttctg
    44101 atataaaaat aactaaagaa gaagttaatc atgcagttga taaagctact gaggaacaga
    44161 atttatttaa aatggagata caagcagcag gtgaaagagc acttaaagaa ataaaagaaa
    44221 gaaatatgaa ggggattgtg ttatgtggaa gaccatatca tatagaccca gaaataaatc
    44281 atggaatgcc agagcttata aattcattag atatggcagt gcttactgaa gactctatat
    44341 gtcatttagc caatgtacaa agaccactta gagttgtaga ccaatgggta tatcattcaa
    44401 gattgtataa agcagctagt tttgtgagag ataaaaacta tttggaactt gttcaactta
    44461 attcatttgg ctgtggatta gatgcagtaa ctacagacca ggttcaagaa atacttaatg
    44521 aaaaatcgaa gatatatact attataaaga ttgacgaagg caacaattta ggagcagcta
    44581 agataaggat gaggtcctta aaagcagcta tgtttgagag agaaagcaaa aatatagatg
    44641 taaaaaatat aaaggtaaac aggatacagt atgctaaaaa taatcagata acgaacaaac
    44701 atactatatt agcaccacaa atgtctccaa tacatttcca attccttgaa aaagctgtta
    44761 acttaagtgg ttataatata gaaattctaa aagatactga tagtagtgta attgaagaag
    44821 ggttaagata tgtaaataat gatgcatgtt atccagctat aatagttgta ggtcagctaa
    44881 taacagcatt aaaatctggc aaatatgatt taaataacac ttctgttact ataactcaga
    44941 ctggtggagg ttgtagagct acaaattata ttggattctt aagaaaggct atatatgatg
    45001 ctggatttaa agatgttcct gtcattgcac ttagtgtaaa tggaatagaa gatagtggta
    45061 taatggataa tatatcacta aaattaatca atagactctt tatgtctgtt gtttatggtg
    45121 atttgctgat gaaagtacta tatagagtaa gaccatatga aaaagttact ggaagtgcaa
    45181 atgctctata tgaaaaatgg gttgatatat gcaaatcttc tcttgttaaa gcaaaaattt
    45241 ctgtatttac tagaaatata aaagatatag taaaagaatt tgataattta gagatactag
    45301 atatcaaaaa accaaaagta ggtttggttg gagaaatatt ggtgaaattc catccaattg
    45361 caaataataa tttagtagat atattagagc gagaaggtgc tgaagcagtc gtgccagacc
    45421 ttacaaattt cttcttaagt tgtgccttta atactatata caaacacact catttagaag
    45481 gaagtagaaa gagtagaatg ataggagagg catttatata cataactgga atatatcaaa
    45541 gagtatataa aaaagcactt gataagagtg agagatttta tgctccagcc aatataaaaa
    45601 atgttgctaa aagtacagaa ccagtagtgt cattaggtaa tcaaactgga gaaggttggc
    45661 ttttaacagg tgaaatggtt gaattattaa atgaaggtgt agaaaacatt atttgtatgc
    45721 aaccttttgg atgtttacct aatcacataa ttggtaaggg ttctataaaa gaattaaaga
    45781 ggttgtataa aaatgcaaat ataataccta ttgattatga cccttctgca agtgaggtaa
    45841 atcaacttaa caggataaaa cttatgcttt ctaaggcttt taaaaatata taagatttat
    45901 attttgatat gttaaaagtt aatgaattta aaaatatact ctatattaag tatctataaa
    45961 aaagagttat cctatttttt agggtaactc tttattagtt aaaaaattat atgtatactg
    46021 atattaaagg ggggaagtaa tatgagttta gtatatgatg tttataaatc aaaattagga
    46081 aatttgtaca tattaagtga agatggtttt atagttagta tatacatagg agatgaaaaa
    46141 tttaataatt taaaaaatga atgtattaaa aaaggaattg atataaagag aagtaaggaa
    46201 gatttaaagg aagcaataag ccagatagat gattacttta atggaaaaag aaaagtcttt
    46261 aatttaaata ttcgtattga agggactgaa tttcaaaaat cagtttatag ggaaatgtta
    46321 aaaatacctt atggagaaac attgtgttat tcagatatag caagaaatat aaacaatcca
    46381 aaatcagtaa gagcaatagg acaagcaagt aaaagtaata aaattccaat aataatacct
    46441 tgtcatagag tagttggcaa aaaagatatt ggtggatata tggggaatca ttctgatttg
    46501 aaagagatac tattaaattt agaaagacag agttaactta gaaagaaatg tttttattgt
    46561 taatatttga tataataata gtaaaaagaa gtatttttta caaaaataat aaggaattta
    46621 ttttaattta caaaaatgag gggggatttt aatgtgtgat aaagttatag catcaaaata
    46681 tcatgagaag ggatttaatt gtgcagaatc agttataaaa gcatataatg aggaatttaa
    46741 tacagatata ccagtttgtt taggaagtgg actgggttca gggtgtgggg ttgctagttt
    46801 atgtggtgca gtaaatgcat caaatataat aattggatat gtaaaaggca gaaaccacga
    46861 agatgaaagt actaaagcta aagtatatgc taaagattta actacaacag taagaaaaga
    46921 gtatggttca gagttatgta tagacctaaa aaaagattta gttgcttgta gagaaataat
    46981 ggatttcgca tatgatagtc taaaggaaac tttaaaaaaa gaattataag aaaataagct
    47041 atcaaatata aattatttga tagcttattt ttatagccga tactaaataa atcaaatata
    47101 ttttagaatt attatttgta aataaacagc aaataatttt aggcttttta tttaatatag
    47161 tttttattta tatttatttt tgaatatgtt atactttgtt taaataaata tttgtaatga
    47221 gggagatgag aagattgaag aatctcaatt tacttattaa taaattatat tcaaaaaatc
    47281 ataatgaagc atataaaact tttttattct tagaaaatga aagtttaaaa tcaaatatta
    47341 catattgttt ttttgactca ttcctagaaa tgattgataa cgaaaactca tatattcgaa
    47401 caaggggatt gttattaatc tctgcaaatg ctcagtggga tattgataat aaaattgaaa
    47461 taaatataga tagtatacta agtcatatcg tagataaaaa accttctgta tcaagaatgt
    47521 ttattaaatc catacctaat attacaaagt ataaagaaaa tcttattaat cgcataaaaa
    47581 tggaattaag taatgcagat a
//
LOCUS       DAENCV010000016        46804 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAENCV010000016 DAENCV010000000
VERSION     DAENCV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.016"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            46..120
                     /locus_tag="KQD82_000865"
     tRNA            46..120
                     /locus_tag="KQD82_000865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:tgt)
     gene            complement(280..957)
                     /gene="srtB"
                     /locus_tag="KQD82_000866"
     CDS             complement(280..957)
                     /gene="srtB"
                     /locus_tag="KQD82_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG4379351.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNSESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1089..1423
                     /gene="rnpB"
                     /locus_tag="KQD82_000867"
     ncRNA           1089..1423
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KQD82_000867"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1701..4244
                     /locus_tag="KQD82_000868"
     CDS             1701..4244
                     /locus_tag="KQD82_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG4379352.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4367..6358
                     /locus_tag="KQD82_000869"
     CDS             4367..6358
                     /locus_tag="KQD82_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379353.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6348..6668
                     /locus_tag="KQD82_000870"
     CDS             6348..6668
                     /locus_tag="KQD82_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379354.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6866..7735
                     /gene="galU"
                     /locus_tag="KQD82_000871"
     CDS             6866..7735
                     /gene="galU"
                     /locus_tag="KQD82_000871"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG4379355.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7759..8772
                     /gene="galE"
                     /locus_tag="KQD82_000872"
     CDS             7759..8772
                     /gene="galE"
                     /locus_tag="KQD82_000872"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG4379356.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8884..10818
                     /locus_tag="KQD82_000873"
     CDS             8884..10818
                     /locus_tag="KQD82_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG4379357.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10973..11836
                     /locus_tag="KQD82_000874"
     CDS             10973..11836
                     /locus_tag="KQD82_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG4379358.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(12044..12925)
                     /locus_tag="KQD82_000875"
     CDS             complement(12044..12925)
                     /locus_tag="KQD82_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4379359.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13337..14185
                     /locus_tag="KQD82_000876"
     CDS             13337..14185
                     /locus_tag="KQD82_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG4379360.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14279..16201
                     /locus_tag="KQD82_000877"
     CDS             14279..16201
                     /locus_tag="KQD82_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG4379361.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16283..17146
                     /gene="aroE"
                     /locus_tag="KQD82_000878"
     CDS             16283..17146
                     /gene="aroE"
                     /locus_tag="KQD82_000878"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG4379362.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17346..18563
                     /locus_tag="KQD82_000879"
     CDS             17346..18563
                     /locus_tag="KQD82_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4379363.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVIDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18799..20664
                     /locus_tag="KQD82_000880"
     CDS             18799..20664
                     /locus_tag="KQD82_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG4379364.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWYISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20842..22206)
                     /locus_tag="KQD82_000881"
     CDS             complement(20842..22206)
                     /locus_tag="KQD82_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4379365.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPAAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22384..23727)
                     /locus_tag="KQD82_000882"
     CDS             complement(22384..23727)
                     /locus_tag="KQD82_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG4379366.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23800..23901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24230..24883
                     /locus_tag="KQD82_000883"
     CDS             24230..24883
                     /locus_tag="KQD82_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG4379367.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            25024..26295
                     /gene="brnQ"
                     /locus_tag="KQD82_000884"
     CDS             25024..26295
                     /gene="brnQ"
                     /locus_tag="KQD82_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG4379368.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26497..27684
                     /locus_tag="KQD82_000885"
     CDS             26497..27684
                     /locus_tag="KQD82_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG4379369.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(27914..29938)
                     /locus_tag="KQD82_000886"
     CDS             complement(27914..29938)
                     /locus_tag="KQD82_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4379370.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            30328..30996
                     /locus_tag="KQD82_000887"
     CDS             30328..30996
                     /locus_tag="KQD82_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379371.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            30998..31483
                     /locus_tag="KQD82_000888"
     CDS             30998..31483
                     /locus_tag="KQD82_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG4379372.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            31544..32665
                     /locus_tag="KQD82_000889"
     CDS             31544..32665
                     /locus_tag="KQD82_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG4379373.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            32946..34136
                     /locus_tag="KQD82_000890"
     CDS             32946..34136
                     /locus_tag="KQD82_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4379374.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    34270..34525
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34584..35585
                     /locus_tag="KQD82_000891"
     CDS             34584..35585
                     /locus_tag="KQD82_000891"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG4379375.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            35689..36078
                     /locus_tag="KQD82_000892"
     CDS             35689..36078
                     /locus_tag="KQD82_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG4379376.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVITKKDKHSNIIENVEFSIKDEEE"
     gene            36239..37447
                     /locus_tag="KQD82_000893"
     CDS             36239..37447
                     /locus_tag="KQD82_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG4379377.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(37533..38609)
                     /locus_tag="KQD82_000894"
     CDS             complement(37533..38609)
                     /locus_tag="KQD82_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4379378.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            38838..39365
                     /gene="hpt"
                     /locus_tag="KQD82_000895"
     CDS             38838..39365
                     /gene="hpt"
                     /locus_tag="KQD82_000895"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG4379379.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            39454..40386
                     /locus_tag="KQD82_000896"
     CDS             39454..40386
                     /locus_tag="KQD82_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG4379380.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIAAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNMSD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            40448..41674
                     /locus_tag="KQD82_000897"
     CDS             40448..41674
                     /locus_tag="KQD82_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG4379381.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            41758..41979
                     /locus_tag="KQD82_000898"
     CDS             41758..41979
                     /locus_tag="KQD82_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG4379382.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            42168..42338
                     /locus_tag="KQD82_000899"
     CDS             42168..42338
                     /locus_tag="KQD82_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379383.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(42431..42892)
                     /locus_tag="KQD82_000900"
     CDS             complement(42431..42892)
                     /locus_tag="KQD82_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379384.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            43075..43542
                     /locus_tag="KQD82_000901"
     CDS             43075..43542
                     /locus_tag="KQD82_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379385.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            43585..45348
                     /locus_tag="KQD82_000902"
     CDS             43585..45348
                     /locus_tag="KQD82_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG4379386.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            45356..45964
                     /locus_tag="KQD82_000903"
     CDS             45356..45964
                     /locus_tag="KQD82_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4379387.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            46036..46605
                     /locus_tag="KQD82_000904"
     CDS             46036..46605
                     /locus_tag="KQD82_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG4379388.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT        18180 a         5102 c         8035 g        15487 t
ORIGIN      
        1 taacttttaa aaattagttt tactaattta caataaaata ggtgtgctgg tgtggctcaa
       61 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      121 gtaaagacta tgtttttagc atagtctttt tttattgttt tttattgttt gatgtatttg
      181 ttattttgta ttaaatactt ttagtggtct aatttataaa ttataggtat aaaagatgac
      241 acagtttaaa attattgttt taaactgtgt ctgtgagttt taaatcaatc taccatgaat
      301 caccattctg gcatcatcaa attcatatgt acatgtagaa agtgtaacta ttttatcatt
      361 gctatttacc ttaataggag atttatacaa tgatttagaa gtaatatcat ttatgtaatt
      421 ttgataatca gattcactgt taaaatttgt ttttagataa tcataatcag attctacaat
      481 ataagcagaa aaaacatcat ataaaaattc ttttccattc aaggttattt ttattttatt
      541 atttttttta aagaagtcag catctttaaa cttatttagg ttgttgaaca tagttttatt
      601 tttcatatta tgtccatata ttatgatatt tttgtcatca atagatttat ttctataatc
      661 cataaacagt gtaccagaaa tagaatcttt tttataaaaa tctttgtcaa gatagtaaga
      721 attatcttta gattgtacaa ctggataatt tatatttgta ttctcaacag aaagccaaaa
      781 tttaaaatca ctgttaatct ttgataaatc ctctttttta tattcttttt tttgaagttc
      841 tgaggatatt ttagtatcat gattgtattt ggtaagtttt gagtatatat taaatccact
      901 gtaaagtatt actaatacta gtataatgtt aattactatt ctatataatt ttttcaatat
      961 atcacctcga gttaaatgta gtttatatat ctatattaca ctatttttga ataaatttaa
     1021 atttttagta taatattact ttgacatgta aaagaatttg tgttaacata actaagtaaa
     1081 ataaatatta agtaagccag ataatcgcat gtaatcttac atgaggaaag tcggagctcc
     1141 atagagcagg gtgctaggta acgcctagtg ggagcgatcc taaggatagt gcaacagaaa
     1201 taaaccgcca ctattgtggt aaggatggaa aggcgaggta agagctcacc agcagctagg
     1261 cgactagttg gctatgtaaa ccccacctgg agcaagacca agataggaat aaagagtgct
     1321 gctcgtactc tccggtaggt aggtcgcttg agcctattgg tgacaatagg cctagaaaga
     1381 tgattatcta atacagaact ccgcttatag acttgcttaa ttatttcgat agtcaaaaac
     1441 atcagtttaa actgatgttt ttttatttgt aaatttgtct aatctacttt taaaattttg
     1501 aggaatattg taatataagt tagtacagat attaaaaatg gtataatata agatagatat
     1561 aaaaagaaaa agtaaaatga tgtaaataat atgatatgat ataagatgta gcaaatttag
     1621 taaataatta tttagatatc ataatcaaat tagcaacttt gcacaatgta aatattacaa
     1681 taaaataatt gatttaagga gtgaaggttt tgagtgaaaa taatataata agaaacactg
     1741 ttttaaatgt cacagcaagt tttttaaaac aagagtctaa aataaatgaa aagctagatg
     1801 gtgttttgga aaagaaattt gaaaaagtag aatttaatga agcaaaatat gcagagcttt
     1861 taaaatttaa tatattgttt tataagacat tagcaagaaa tacagaacca cttattggca
     1921 aatggatagt agataagtac atacctgaaa tagatgaatt agaaaaagat ttagaattaa
     1981 ctacagcaaa atgtagaaag tatgtaaata aggctatgaa agagggatta gactctctaa
     2041 aagccaatga tttaaatagt tttttagctt atgataaaat ggaactatca gagagaagaa
     2101 gacgtttaga aaaggattat aaggttttaa acctatataa agaccttctt aatatatctc
     2161 ttagaaaaat aagtttagaa aagaaagaat gtggagattt atttcttaaa aatcaagcag
     2221 atgcgaaaag agaattaaaa agagaaatta tattctgtgt aaacaaaata ttagctagca
     2281 ataaagatgt ggtacctaat aatgtggaag agaataacga acaagacaaa gtagaaataa
     2341 aagcaactga aattgaggat ttgcaattag gtaaagataa ttcagaaaat gacatgctac
     2401 taaatgaaat taaaaattca aaagtaaaac atgaatcaaa atcaaatgat atagaatcga
     2461 agtttataga taagttaaat agcttaaata atgaaagtgt atctcaaaca atacacaatg
     2521 aatataagaa gttatgtgga ttagaagaac ttcattctgt tgaaggatat ggttttggca
     2581 aagaaattat taaagatttt gcttgtgcaa cggttgtatt agaattttta aagagaagaa
     2641 atagagacct tatagaaggt gcaatgagac tgactataat tggagaattt ggtcctgaaa
     2701 attttaagga atttgtggac tatgtaatta aaaataaaac agaaattagt gaagatacat
     2761 ggaataaagc acagaatcta ataaaagata actatagtga attagaaaat catgaaattg
     2821 catcaaaaag aactagaaga aataaagata tcgatattga agaatacatt tatatgataa
     2881 aaaatgcaga taaagatatt tgctttagaa gtagtatttc aatagaagat gatgctgaag
     2941 aaggggttaa agaagaaata gatagtaata atcaagatat aggtgacgtt gtagaagata
     3001 aagatactac agataaagag tacgacagca ataaagaaga tataattgaa ccagaaaata
     3061 aaaagagtaa aaagaaggct aaattatttg gatttattaa aaaagacaat gaagaagttg
     3121 aacaagaaga agagaattta aatgatattt ctcctgatat aattttagat aaaccagttg
     3181 aaaatcatca agtaaaatca gaagaaatgg agcaaaatga attaaaagaa ataaaacaaa
     3241 aagaaccaag tcaacatata gaagaagaac gtaatgtaaa aatagaaaaa ccaataaata
     3301 ataacttaga tgaaaaagta tcttcaaata atgaatcgaa gcttgaaaaa gaaagtaaaa
     3361 acttagaaga taaaaaagca aaagagataa aagaagaaaa attagaaaat gaaaaatcag
     3421 tagtaatacc tattaaaaag aaagaaaata gtaataaaaa atctaaaaat tcttctaagg
     3481 acaaatatag agaaaacaag aaagaaatga aaaattatgt atcagataaa gaagattctt
     3541 tagatgatga agaggttgtt tctaagaaga gtagattaaa agaaactatt atagcagtag
     3601 ttatagtggt tattgttggt gtaggatatt ttataacagt aggcaacaat aagaaaaatg
     3661 ataaagagaa tattccaaag tcatcaactc aacaacaagc taacaataaa ttaactgaag
     3721 aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaag gcaaaagctg
     3781 aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaagaaatg gaagcatata
     3841 aagatggtaa aggtgtatat tacactgttt atgcaggttc acttaaagtt gaaaaaacag
     3901 caaaagaaac agcaaaagaa tatgaggcta aaggtatttc ttcaacaata attcaagaaa
     3961 atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca caagaaaaat
     4021 gtaatgaact agctaagaag tctattgata catatatagc tatgtatgat aagtactatg
     4081 attataagtt agaagaatta aaagaatcag ctccttcttt gagtgctgaa gaacttaaac
     4141 aaaaatatga agatttaaga agtgagttaa agaacaaatc tggatatcgt gaatatgtta
     4201 aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagcatt ataaaaaagg
     4261 agaaactatg tctcagaata aaaagtatta aaaaaaatat tatttgtttt gagacatttt
     4321 attcaccttt ataaaatact tataattctg tttaggtggt gaaggtatgt tgaaattagg
     4381 agaaaaaatc atatatgagc tttcagatgg gtttgagctt agtggcataa gtagatactc
     4441 aaaagaatat aaaaagcttc atataacaaa tctagggaat ataataatca gcaaatcaga
     4501 tgaagttaat gaggatacag gtgtaaaata tatatatagt ttttcagaca ataaagaaaa
     4561 attagaggca gttttagaag attctcaaat aattgtctat ggaaataact tgccttttta
     4621 cataaaaagt gaaaattcaa gtaaaaatgt tacagcaaat ttgactatga aaataacttt
     4681 ggatgaatat gatttaatct gcaaaagtca aagagaattt atattctatc taaaggataa
     4741 tatgactata ttaagtttag atgacaataa gttttatatg ggtggaatta ataaagataa
     4801 tgagaagttt atatttatca gtgggaaaaa tagatttgaa ataaattttg atgatataga
     4861 aagatacatt ttagaagata aaagagtgtc attgaaggga tactttcata tggaaagaga
     4921 ggggattata gtaaggtctg tatctatatt taataacaat atagatagtg tagtcccatc
     4981 tgatttaaat gagagagtta aagataacca aaaaatagga aatcttccta aagattgtga
     5041 aatagtattc tgcaagatat caggaaatat agatggattt gattataaaa acacaaacat
     5101 gcttttagta aaatatcaag accaattaat ttttataaat aagaaatcta agaagactat
     5161 tgtaaagtca gcgaaagata attgttctaa gttaaattta ggagaagata taatattata
     5221 tgataataaa aatgtattta acctacatat aaatgataaa aatagagaaa ttatgcaaat
     5281 agatgaccta aaagatatag aaaatgaaat agtgggatat acattaaaac atgctccatt
     5341 ttttattcaa gaagatttcg atagcttgac tattctaaag tcttttcaaa aagaaatcat
     5401 atctataaaa aatagtgata taaaagacat tgtaattaat aaagaacttg aaaacgaaaa
     5461 ttctaatttt gtagaaacgg aaataaaatt taataatcaa aaagtacttt taaacttaag
     5521 taaaagtatg gttcaaaaac ttatgcaaga tgtatttata tatgcaaagc aacctctact
     5581 taaagaaaat tctattgaag tgatatataa gaattggtct aaagctatga atgatatgat
     5641 aatatttaat ttctttggaa atatttacta tatgaaatct gaatttgata agattttaga
     5701 aaaagagtta aatgatgaaa taagaataga agttataaat tcactttata agcaaataca
     5761 agaacaaaga aacaatttag atttattgtc agcatatatg ccaagaatat tagaaaatca
     5821 agaaatagat ttgtttgaaa agtataatac taaacttgat gtacaagtgt ttaagcagat
     5881 taaaaatcta ttgtctgatt tatcatataa tataagcagt tatttaaatg aagttgaaaa
     5941 aagtttagac aatatcatct ttgtaatttc aggagaagat aagaagaaat acaattatag
     6001 aatgttaaaa gaaagtgaaa gtgcaagtct tgatgtattt ttaaaacaag ccatatcaag
     6061 attaaaccat ttagtagaaa atatgtatcc ttattatgta gatgaaacta gtagggaaat
     6121 gtttaagtta tttgagttat tgtggaaaaa ctatagaaat attgatgatg atagtataaa
     6181 agaaatatta ttcgagagaa taacaaatac atatgtattt aaacaattga cattaaataa
     6241 tagtacaaaa gaaagaagaa aagacataat tgaaaaaata tataactctg ttgattatgg
     6301 aaccaataaa ctagatgaaa atatgttttt cacaggagga ataaagtatg tcaagtaaat
     6361 acaatgtttg gacttttaac tatgagttct taggattgga ctcaggggat gaaaaaaata
     6421 atgaagttta ttatgagatt gatttagatg aaattttaaa tgaaaactat atagatgaag
     6481 atatagatag tggtttagat tctgatggaa atagtatttt aggagaatta atatcagaaa
     6541 tggatataga tgaggatgtc agtgttgata tgacatatca agaactagaa gttgacttag
     6601 aagatttaga ttcttatcta gatgaagata tagatagtga tataaaaggc atcttagatg
     6661 aaatttagta cctgtataag ttttggataa aatataaaca atacaatatt aacttataaa
     6721 actaaaggta gatgaaattt catctacctt atattatctt atagcattaa atattgggat
     6781 aatatagagt ttgaagaaaa agtataaata tggtatatta atttggtaca atagaaaatt
     6841 tacgaagaag ggtgaaaaaa tgcagatgac agttaaaaaa gcaataatac cagcagcagg
     6901 gctaggaact agatttttac cagcaacaaa atcacagccc aaagaaatgc ttcctatagt
     6961 agataaacca actttacagt atataataga agaagctata gaatcgggaa ttgaagaaat
     7021 tcttataata acaggtagaa ataaaaaatc tatagaagat cattttgata aatcagttga
     7081 attagagtta gaactagagc aaaaaggtaa gacagagatg ttagaaatgg taagagacat
     7141 atcaaatatg gtaaatattc attatataag acaaaaagag ccaaaaggtc ttggacatgc
     7201 aatttactgt gcaaaaagtt ttataggtga tgaacctttt gccgtattat taggtgatga
     7261 tatagtagat tcagatgttc cgtgcttaaa acaattaata gatacttata atgagtataa
     7321 aacaactgta ttaggtgttc aaaagatagc taaagaagat actaataaat atggtatatt
     7381 ggatgtaaaa catatcgaag atagagtata taaagttaaa gatatggttg aaaaacctgc
     7441 aattgaagaa gctccatctg atatagctat acttggaaga tatataataa ctcctgcaat
     7501 atttagcata ttagagaaac agacacctgg taaaggtgga gagatacaac tgacagatgc
     7561 acttcaaact cttggaaaac aagaggctat ttatgcttat aactttgaag gtagaagata
     7621 tgatgtggga gataagattg gttttttaga ggcaacaata gactttgctc taaagagaga
     7681 aaatttaaaa gatgatttaa tggactatat gagaaaaaaa gtaagtgaaa aataaatatt
     7741 agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata tataggaagc
     7801 catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga taacttaagt
     7861 aatagcaact caatagtggt tgatagaatc aaggaattat ctaaaaaacc agtaaaattt
     7921 tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga aaataatatt
     7981 gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga aaaaccaatt
     8041 gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat gagagaatat
     8101 ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc acatacttgt
     8161 cctatattag aggatttccc actttctgta accaatccat atggaagaac taaacttatg
     8221 atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat agctctttta
     8281 agatacttta atccagtcgg tgctcataaa agtggtagaa taggtgaaga accaaatgga
     8341 gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt gaaggaattg
     8401 agtgtttatg gtaatgacta tccaacacat gatggaactg gagttagaga ctatatacat
     8461 gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga aaatccagga
     8521 cttgttgttt ataatttagg aactggaaag gggtatagtg ttcttgattt agtaaaagca
     8581 ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag ggctggagat
     8641 gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg gaaagctaag
     8701 tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa tcctaatggg
     8761 tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa atataaggta
     8821 ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga gggggaagtt
     8881 atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt tttagtattt
     8941 gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt aaattcaaga
     9001 aaaaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag agttgcaaca
     9061 ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa gataaaaaat
     9121 agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt aggagataga
     9181 tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca tggaaataat
     9241 aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca caataaagat
     9301 gttagatggt tatttgacca agttcctgta ggttcagatg taattataga ttatagtaac
     9361 gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac aggatggaaa
     9421 acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa gaatagttgg
     9481 ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca aacaggatgg
     9541 aaaacaatta ataacaaaaa atattattta ggaactgatg gagcaagagt atctggatgg
     9601 aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca aacaggatgg
     9661 caagaaaaga atggaaagaa atactatcta ggatctgatg gtttagcagt aacaggatgg
     9721 caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca gacaggatgg
     9781 caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct aactggttct
     9841 caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa tcctacatgg
     9901 gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc agttggaaac
     9961 tggaatgctg atagctcaga tactgtaata ttagtaaatg gtaatgcaat agctgatggt
    10021 ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac taatacagct
    10081 aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac agtaatcttg
    10141 ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac tactttcaac
    10201 acagaaacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag aatagctgaa
    10261 gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa tggggaggca
    10321 gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat attagtcaat
    10381 aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt agaaaatgca
    10441 tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat gaatgatata
    10501 actactgaag atatttcagg aaatagaata tatggagata gtagagtaga tactaatgcc
    10561 aaagtaatag aaaaattcta tggagatact gatttacagg ctgtattagt tagtaaatca
    10621 gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca ttcaccaata
    10681 gttttaatgg ataattcagg attatcatca gagcaacaaa gagtatttgc aaataaaaaa
    10741 gttgaaactc cttatcaaat aggaggcgga gtatcttata tagtaatgga taaattaatg
    10801 gatattcttg caaaatagta aaaatatata ttgaaaaata attataattg agatgtgaaa
    10861 ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa attaagttta
    10921 agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat agatgctatc
    10981 atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt ttattgatga
    11041 aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag taatttttga
    11101 atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag aaatggatgc
    11161 atttaaagag tattgttttg gaaccagtaa cataggaata catttgacag atatatcaag
    11221 aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct atgatgagtt
    11281 gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag ataaagctag
    11341 attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta tacatgttct
    11401 tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa tagttataga
    11461 gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg tatttaataa
    11521 tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg gttttataat
    11581 tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata ttttatgtaa
    11641 tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta tgcaatatgg
    11701 agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa cgagtgtgaa
    11761 tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg gaccatggct
    11821 tgaaaaaatg cgttagatac aattgacaaa ttttaaaaaa tctgatataa tgtaagtaac
    11881 ctagttattc aggaactgaa tccagttgct gttatataac gataattact gagactcgga
    11941 tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta ttttataaaa
    12001 agaataaaag aggatattta aaaataaccc ctttattaat aaattataat tgagcgtttt
    12061 taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct tttataaatg
    12121 ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt acttttgtaa
    12181 agctaagcat attcatattt gggactacag caattccgaa attttttgca actaatccag
    12241 caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt atttttttaa
    12301 acatatcatc aattatatat ctaagaccgc ttctctgatt aaatactatt tgaggataag
    12361 gtatggtttc ttttaaatct atagtatcct ttgcagctag tggatgacta tatggtacaa
    12421 ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct ttttcaagtt
    12481 tcgaacaaaa tgcaatatca tattttcttt ctttaagacc ttgaataatg tcagtagtta
    12541 caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa aatttgtgca
    12601 ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct atttgtcctt
    12661 caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct aatgattttt
    12721 ccacatcttt taaaaatatt tttccatact tagtaaggac tatatttcta ccttcttttt
    12781 caaataaagc caccccaagt tcattttcta gtaaagaaat agcatgactt aagcttggtt
    12841 gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc aatgtaacaa
    12901 aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat tttatttata
    12961 tatttgtaga agttaacctt taagatattt ttacattttt attatatatg attatatagt
    13021 aagaataaaa gcttttaaaa aaatttatcg ttttttgtct gaattataag ataaatatat
    13081 ttatacgacc taatagaaaa atacaagaat tatcttataa ataaaatcta taaacaaaat
    13141 ctatgaatta ataggaaatt atcaatttgt tattagtaaa gtctcgtgat acaataaatt
    13201 tataaaatct agataaatag aaatcaaaaa atacttttat ttttaaggta aaataatact
    13261 actagtagga taaataaaaa tataaaatca aaaatactat taattataaa gaaaataaag
    13321 ggggcaaatt taagctatga ataatcaaaa aaatcttaat attccaatta caattagttc
    13381 ttatacattg ggaacagaag tttcattccg tgatagagtt cgtattgcaa aagaagctgg
    13441 gtttgatggt attggattgc gtgctgaaaa ttatattgat gcaaaaaatg caggtgttac
    13501 agatgaagaa atgattgcaa ttcttaatga atatgatatg aaagttacag aagtagaata
    13561 tataactcaa tggggaagag aagaagatag aacggaagct caaagagaaa aagagcaaaa
    13621 tgtttatcat atggctcact tatttaatgt taaacatata aattgtggat tattagagat
    13681 gataccagaa gaacaaatta taactgcatt gggagaatta tgtgacagag caggtgaact
    13741 tattataggt cttgagttta tgccatatag tggtgtacca gatttggcta ctgcatggcg
    13801 tgttgtgaaa ggatgtaatc gtgaaaatgc aatgcttata ttagatactt ggcactgggc
    13861 tagagcaaag cagacagcag aggaccttgc acctgttcca gcagataaga tagtatcaat
    13921 tcaagtgtgt gatgttttag aaactcctta tgaaaaactt agggatgaat cactacatga
    13981 ccgtttggct ccaggtgaag gatatggaaa cacagaggaa tttgtgaaaa taataaaaga
    14041 acacaatata tcaccaagag tcataggggc agaagtaata tctgattctc taatatctaa
    14101 agggttatcc actgctgctg aaactgtatt taatgcaact aaaaaagttt tggataaagc
    14161 atggtctgaa gtatctccaa aataaactta tataatataa aaatatacag agtattataa
    14221 tataaaactt ttaaatataa aacttttaaa tataaaactt gaaaggaagg aaaaacttat
    14281 gaagattagt tctatgttta cacctataag aattggttca atgacagttc caaatcgttt
    14341 tgtagttccc cctatgggaa acaattttgc aaacacagat ggtactttaa gtgaaacttc
    14401 taaggcttat tatcttgagc gtgctttagg tggttttgga ttaataacta tagaatctag
    14461 tgttgtagac aaaaaagcaa aaggaggtcc tcgtaagcct tgtttatatg atgatagcac
    14521 aatagatagt tttaaaaatg taatagatgc ttgtcatgat gcaggttcga aggtatctat
    14581 acaattacaa catgctggtt ctgaaggaaa tgaaaaagta gcaggacatc cattaaaagc
    14641 tgcttctgca atacctgcat caaatggtcg taatacacca ctagcaataa caacagaaga
    14701 aatatatgaa cttatagaat cttatggaga tgctgcatta cgtgcacaaa aagctggtgc
    14761 agatgctgtt gaagttcact gcgctcatgg atatctggtt agtagtttta tatcacaaag
    14821 aacaaataag cgtgtagatg agtttggtgg atgttttgaa aatagaatga gattacctcg
    14881 tcttatcatt gaaaatatac gtaaaaaagt aggaaattct ctagcaatat tatgtcgtat
    14941 aaacagcact gatgatgttc caggaggaat tgatgtacac gatagttcag taatagcagc
    15001 atatctagaa gattgtggaa tagatggact tcatgtttca cgtagtatac atatacatga
    15061 tgaatatatg tgggctccta cgactttaca tgctggattc agtgcagaat tagttactga
    15121 aataaaaaaa gctgttagta taccagtaat aacagttgga cgttatacag agccacagtt
    15181 tgctgagtta atggtcagac aaggaagatg tgatttagta gcattcggta gacaaagctt
    15241 ggctgaccca gaaatgccta ataaagctaa aaatggaaaa ctagacgaaa tgataccatg
    15301 tattgcatgc ttacaaggat gtgtaccaaa tatgttccag ggcaaaccta tagcctgtct
    15361 tgcaaatcct atattaggac atgaagcaga gcttaagcca gctgaaatta gcaaagaagt
    15421 tcttgttgtt ggtggaggtg ttggaggtat gcttgcagca tgggtatgtg ctaaaagagg
    15481 acataatgta actttagttg aaaaatctga agttcttggt gggcaaatgc gtttagctgc
    15541 atatcctcca ggaaaaggag atattactaa cttagttaga agttatattt caaaatgtaa
    15601 tcaatatggt gtaaaaatat gtactaatac agaagctaca gtagaactta taaaagagaa
    15661 atcacctgat gtagttatta tagctactgg agcaactcca cttgtacttc ctataccagg
    15721 aatcaatgat tcaggattaa ttcatgctgt agaccttctt gatggtaaaa aatcttgtgg
    15781 gaaaaaagta cttgttgttg gaggtggaat ggttggatgt gaagtggcag ccttccttgg
    15841 agaacaagaa catgaggtta ctgttattga gcttagagaa gaagttggtg cagatgtaat
    15901 ttcagaacac cgtaaattcc ttatgaatga ttttgaaaat tataatatac ataccattac
    15961 tggtgcaaaa gtttctaaat tttatgatga tggtgtcgac tatttactta cagatggcac
    16021 agagcatgat ttaaaaggtt ttgattcagt agtacttgct atgggttctc gtaattacga
    16081 cccattaagt gaagttataa aagaagttgt aaaagaaact tatattgttg gtgatgcagt
    16141 aaaagctcgt agggcattag atgcaacaaa agaagctttt gaagtagcat taaatatata
    16201 aataataaat aaatttttct ttgtaaataa ctgaataggc tatttgcaaa gaaaatgata
    16261 atattgggag gagattatag ttatggaaat ttcaggtaga acaggattat ttgcattaat
    16321 aggtacaccg gttggtcact ctaaatcacc agtgatgtat aattatagtt ttaagaaatt
    16381 agatttagat tatagatatt tagcctttga tatcacagta gataaggtaa aagaggcact
    16441 tttagcaata aaaactttta atataaaagg agccaatgtt actatgccat gcaaatctgc
    16501 tgttacagaa tatatggatg aactttctcc agcagctcgt ataattggag catgtaatac
    16561 aattgtaaat gacaatggaa agttagttgg tcatataaca gatggagtag gctatgttcg
    16621 taatttaaaa gaaaatgggg tagaagttaa aggtaaaaag attacaatta tgggtgctgg
    16681 tggtgcagca actgctatac aagtacaatg tgctttagat ggagcaagag aaatatctat
    16741 ctttaatcct aaggatgatt tttataaaag agcagagcaa acagttgaga atataaaaaa
    16801 agatgtacca gaatgtgttg ttaatctata tgatttagaa gatacaaaca aactttatga
    16861 ggagatacaa tcaagtgata tattgacaaa tgcaactctt attggtatga agccctatga
    16921 caatgaaaca aatataaaag atactagtgt tttaagaaaa gacttagtag taacagatgt
    16981 cgtttataat cctaagaaaa caaaaatgat tgaagatgct gaagctaatg gatgtaaggc
    17041 tattggtgga ttaggaatgt tattatacca aggagcagag gcttttaatc tatatactgg
    17101 attagaaatg cctgtagaag aagttaatga attatgcttt aaatagataa gagtaatatc
    17161 ttaaacaatg aaaaaacata cgtatttcaa tatttttaat aataaattac aaattgtcat
    17221 agtgggtttt gcctcatagt ccagttaaat ttgaagcttt aactgactat actttaaatc
    17281 cattatgggg atgagtttat tgtacacaaa aatgttaata aattaacaag aatgggagaa
    17341 aaattatgaa caataaaaaa tattatccta ctgccatagc gttgtatttt tcatattttt
    17401 tacttggtat tggaatttca attttaggac aatacaaacc agaattttca tcaatgtggg
    17461 gggctaaaac attatcagat ggaacattgg atgtaagtat tgttttagct gttattgctg
    17521 cactaggatt agggagactt atatcttatc catttgcagg acctatttca gataagtatg
    17581 gaagaaaagt aagtggtctt ataggaaact ttctacatgc aattttcttt gttggaattg
    17641 tattttcacc aaatttttat atagcatatg tatttgcaat aattggaggg gcagcaaatt
    17701 cattcttaga tacctgtgta actccttctt gtatggagat atttgctagt ttaggaacaa
    17761 tagcaaatat gtttactaaa tttacaatag ctttagcaca atttttgctt ccttttataa
    17821 ttggatttgt tgcagcaaat tcaatatcat ttaaagttat ttttatagta acagcgattc
    17881 ttattgtaat agatgcaatt ttgatagctg tgttaccatt tccaccagct aataatgtta
    17941 ttgataacaa aggcaaaaca gttaaatcag agaagatgaa atttacacca acaagtatag
    18001 ctcttgtttg tattggattt acatgtacat ctacatttgt attatggtta aattgtaatc
    18061 aggaattggg taaattgtat gggatggctg accctacaaa aattcaatcc ttttactcaa
    18121 tgggagtaat atgtgcagta ttaataactt ctttacttat taaaaaatat attaaaccaa
    18181 taagaatatt agttatatat cctataatag ctttacttat gctattagtt gtatattttg
    18241 ttcaaactcc aactatttgt atgttagggg gatttgtaat aggttattca gcagcaggag
    18301 gtgttcttca gttaacaacg tcaactgcaa atgaaatgtt tccaactaat aaaggaaaaa
    18361 taacatctat agttatgatt gcatctagta tagcaaacta tgttatttta aatatagcag
    18421 gtattattac aaaatcaggg ggagttaatg gaccaaaata tgttgtattg tttaatgtgg
    18481 caataacatt tgttggaatt ttacttgcat tgtttgtaaa catgagatat gaaaaagaaa
    18541 aagtgtatga ttatgatgtc taatattagt ataatatacg catagagaag tctaaatcta
    18601 ggatagattt ttattaaaag taattaaaat taataaggat attatttaca gttataagat
    18661 agggtattta cataattaat aataaggata ttactagaat ttcaatttct agtaatattt
    18721 ttttgcgtat aaatgtaaac ttgacgaaat aagtagtata atcaatataa attacatcat
    18781 gtaaaggagg ataatttggt ggagaatgtt tttaagcgat tgcaggaatt taatggttat
    18841 gatggataca aagaatcatt tgaaatgaat tacctatgta tatatgaaag tataccgtta
    18901 agagaacaag tagagcttgc aaataatcta gtagatgaaa ttttaaatat gtataaaagt
    18961 gaatctaatg aaatttattt attggaggat agtaattcta agtctttaat ttgttatttc
    19021 gaaattttta tgaaaaagat aaatactttg gtaaaagaaa tgattattga tgagaaatgg
    19081 ttatataaat taactaaaga attaatatat aagagtaaaa aagttgagta tgtaaaacta
    19141 gggttagttt taagtgagaa atatctaaat gtagaaaact taagagaagt tgtagataca
    19201 ttttctaaat ctggagaata cgtattttat ctgagtaata ctattaaaaa acttgaattt
    19261 tacaatacat atttatttaa tttatctaaa aaggctacag gttcaataaa agtatttgct
    19321 attgttaata tggaaaattt agattctaaa ataaattcct atctaattga agatggttat
    19381 aaggatacaa agtacgaacg attattgatg aactatatta tttcaattgt agatttgaat
    19441 gaatatcttg aaaaacgaga tttagataaa gaaaagataa ataatttagc acgtcttata
    19501 tgcaattact tgctaagtgt agaatttaaa tatataggta ataaattaga attagtgaat
    19561 cgatttttac ctactgtagt aaattatggg actaactttg aaagtttata ttcaattttc
    19621 ctaatagcta taaacgtctt aaaagatgaa aatatagagt gcaataagat tgaatttgaa
    19681 aaagaaataa atggtatctt gctcagtgaa aaatggaaaa atatatattt tgaagcatta
    19741 agagatgcta gtggaaaaac agaagatatt ataaaaatgt cagaaattta tgatgtaaac
    19801 ttatcttttg atgacttgtt gccatatctg aatagagata ttagagattt tgaggtatat
    19861 tggtatatat ctaaaaaggg aacaacaagt tctagattaa aattattaaa tttttttgaa
    19921 gagacgttta aaatagatga tttaattgga aaaatgaaag atatagaaaa agataaattg
    19981 actcaagaat attatgatga tatgttattt tttattgtgc ttaaaggaag taaatcacta
    20041 tatcctgaag gaaagaatat atctttaaaa ggaatatttg gaaatataaa tgaagtaaga
    20101 aaagaatcca taaatatctt aaaaagatac agagagaaac tatctcttga agaattaaaa
    20161 attgttaaag aagcgtatga aaaagaaaaa aatgttatat tgaaggatga attaagaaga
    20221 gtattatatg aaagtaataa tttaaaaaaa gaatttgtaa atattgaaaa aataaaagta
    20281 gatgaacatg gtaaggatat ctatttgact tcaatagctg ttgctggttc aaggtttaga
    20341 aatagagaat atcttgaaaa agaattggag aaaagtaaga tatactattt aactagagag
    20401 aaagataatc tatatgatga aaaagctatt aaaatagttg gtgaaactgg atatgtaata
    20461 ggctatgtcc caagaaaaga aaactatatt ttgagcaatt tattagatgg aggaaaactg
    20521 ctgtattgta gagtaactga atataattta tatgaagatt gtatctacgc aaatgtgtac
    20581 ttgagttata aagatgtaat tgaaacagtt gaaaactctt taaagatggt tttagataag
    20641 agtagaataa aacttataaa ctaagacgtt tatatattgc ttagatgatg taatcttaac
    20701 tgattggtta ttttttctgg aattatggtg tattttatga gcaatgctaa tgaatataaa
    20761 ataaaaaaac aattgttttg tatatatgat gtatataaat atatttatat attcatattt
    20821 acaaaacaat tgaaatttat gttattttat aattttaaat ttagatggaa ttttaacttc
    20881 tgctttatct tttattttag aagcttgttt tttaacttta tttaataatt cagattcatc
    20941 tacaactaat acttttcttt ccttcattaa ccatttacca tcacaaatag tagagtctac
    21001 attttcacta gtcatagaat aaactatatt tccaattgga ttatgcattg gtaaacaatg
    21061 aattgtatct ggatttagaa taatcatatc agcttttttt cctacttcaa gactaccaat
    21121 ttctttttct agtaaagcac attttgctcc atttatagta gccatttcta agacttcttc
    21181 agctggtacg acagttgggt ttaaagttct tcctttatgt attagtgatg taagatacat
    21241 gtccctcata aggtccattc tattatttga aggagctcca tcagtaccaa ttgaaacagg
    21301 tattcctttt tctaacattt caggaattga tgcaaatcct agaactactt tcatggctgc
    21361 agctggatta tgagaaactt ttacatcatg taatctaaat aaatcaattt ctttatgagt
    21421 aagccaaact gtgtgagcag ctaaaagatt tggtcctaat acacctagtt tatcaagata
    21481 ttcaactgtt ccaactccat tgttttgctt tacaaatgaa atttcatcag ctatttcagc
    21541 aatatgcata tgtatacctg tattgtattt atcagctaat tctttagttc taacaagtaa
    21601 ttcttcagtt gtattaaaaa tagttcttaa tgcaaaccat attttaatcc tctcattaga
    21661 tgtgttatta aacttatgga ataagtctaa ttgagtgttg atttcctcat cagtagtttt
    21721 ttgccatgct tcaggaagac catctcctgt atccataact gatttagcta aacaggctct
    21781 aagtccagtt ttatcaacag cttcaaccat agcatctaca tattgaccac cagattctaa
    21841 aaatgtagtg accccagatt ttatcaattc aacacaacat gcagtagatg aaatcaatga
    21901 atcttcatag ttgaagctac tttcgtatgg ccagattctc tctctaagcc aagttaaaag
    21961 tacaacatca tctgcaagac cacgtcctaa ttgttgggat aagtgaacgt gtgtattgac
    22021 taaccctggc attagtattt ttcctttagc atcatatatc tcagcgtctt tatctacaat
    22081 attaatatca aacttaccaa cattttttat aatattattt tcaataagta tacttccagt
    22141 ttcatatacc tctctatctg cattcatagt aattagatat gcatttttta taagtaattg
    22201 tgacataagt acaacctctt ttctatatat ttttatatct atgtattatg ataataaatt
    22261 ttctatacaa agttaatagc aaaatcaatt tatacataaa ttgattttgc tatttataaa
    22321 tttaaaataa ataagatttt attctatata atttaaaata ttgacctaga actatttaaa
    22381 atattaactt agaactaagt ttacaactgc aagtaaacct ataatccaaa gtgtaatact
    22441 gattttatca gtttctttag ctaccacatg cattattata tatgaaataa atccaaagca
    22501 taaaccaata cttatactat aagttaaagg catcaataaa actgtaacaa aagcaggtac
    22561 agctattttg ctatcagcaa aatcaatctg ttttatgctt tcaaacatga atacaccaac
    22621 cattacaagt gcaggagctg cagcgtaagt aggaactata ccaagtaatg gtgtaaatat
    22681 aagtgtagct aagaataatg ctgctgttac tattgaagca agtcctgttc ttgcacctgc
    22741 tgcaatacca gaagcagatt ctccaaatgt tgtaacagta ctagttccta agaatgagcc
    22801 tattaaagta gaagaaacat ctgcatataa cattcttcct aaatctttta tttcaccatt
    22861 ctcatcttca agacccattt gtttacaaca agtaataaga aatgctaatg aatcaaacat
    22921 gtctataaac atgaaagaga atataggtcc aattaatgag aatttcaatg cacctacaat
    22981 atctaattta aaagcaactg gtgctatact aggaggtgtt gagattattg ctgttggcaa
    23041 ctccacaaat ccaaatataa cagatattat tgttgcaact actatactta tcaatattcc
    23101 acctttaact tttttatatt ctagtattgc cattaagata agagcaaaaa gggctattaa
    23161 aactgttggt gtaaaatctg caagtgaaac tatagtatct ggattagcta ttataacacc
    23221 catatttttt aaaccaacaa aagcaaggaa aagacctata cctgctgaag atgctactgc
    23281 caattctcta ggaattgcgt ctgcaattgt ctttcgaaga cctgttactg cgagtgtaaa
    23341 gaagacacat ccagatagaa aaactacccc aagacctgtt tgccatggga ttcctgcacc
    23401 tataaccaaa gagtatgtga aaaatgcatt taatcccata ccaggagcta gagcaaatgg
    23461 tgcatttcct aaaatcgcaa atataagtgt accaatggct gtagctagta tggtaactgt
    23521 tataagggcg gctttatcca ttccagcttc agataatatt gctggattta cgaaaactat
    23581 gtatgccatt gttaagaatg tagtaactcc acctattact tctgttttaa ggctagagcc
    23641 acgttcgcta aatttaaaca ttttgtctaa aaagcttaat gatgaattat ttccacctgg
    23701 ttgtacattg ttagtgtttg gtaccatttt tacctccaaa aaactttaag atataaacaa
    23761 aaaaatatat attcatatag atagaataga acatatattc agagttgaca ttctattcat
    23821 agaaaaccca tttaaggtga gtttgtagaa actattagac cgtattacta atattatatg
    23881 aacattttct atttgttttt ctgttgtatt atatcgtaat attgtattat ttacaataaa
    23941 tataataaaa ttattcgttt ttttgtcgat tttataaaaa attaaggtta tttattgaaa
    24001 aaaattactt tttccaataa ttgaatatgt atgagtataa tgtcaattat tctaacttgt
    24061 tttatactat taatggaagt ttattataaa tgagtttgtg atataattat aaaagtactg
    24121 aatacatgga tatttcgtaa aaacttttta atatatcaaa tatgtatatt aagttaatat
    24181 attaaaaaaa ataatatcaa aaataatata atttatagtg gaggggaaaa tgactaaaat
    24241 tattaaagtg caaaaaccta aatttacagg agaattagaa attattgaaa atcttgaaga
    24301 tatacttgaa gatatattta atgatgaaaa aatatttaat aaaattatag aaaatgaagt
    24361 tataagtgat ttagattctg taagagttag ctttgaatct tgtatattta gaaatgtgtc
    24421 ttttgaaaat tgtaatttaa aaaagattga tttacttgat gtggtatttg aaaactgcaa
    24481 tttatcaaat tcattttttg atggaggaag tatatacaga gttgaattta aaaatagtaa
    24541 attgatagga gttagatttg atgattgtat acttaaaaac gttttgttca aggatgtttt
    24601 aggtaagtat tctaactttt cattctctaa atttaagata gtttctatct tagattcaaa
    24661 ttttgaaaat tctgtatttc aagaagtaaa aggtgacaat ctagtatttg agtatacaaa
    24721 tttaaaaaaa gcaaacttta atggaactaa actttctggc atagatttta ctactaataa
    24781 tattgaggga attgagattg gtatagatga tattagagga gcaatttttg atgtgtcaca
    24841 agcaattgat ttaacgaaat tgatgggaat tatcataaaa tgaaaaaaaa attcataaaa
    24901 caaattggga aaacgttaac aatgtgtgaa tttttaccag attgacattt ttctgaaaat
    24961 aaagtaagat tatcttaata ttcaaaatat tttcaaaggg ggtaatttag tgtgagtaag
    25021 cagatgagag atatttttat agttggtttt gctttatttt ctatgttctt tggtgcagga
    25081 aatcttatgt ttcctccatt tttagggatg gaatccggca aagactggtt aattcctctt
    25141 attggatttg ttgttgcaga tgctggtata gcaatgcttg ttattatagc aacagcaaga
    25201 tgtaagggaa gcatggatga tgtacttagg agagctggta aagggttagc aagagtaatg
    25261 agtgtagctg ctctagcatg tttagcactt cttgtaatac caagaacttg cgcaacaaca
    25321 tatgaaatgg gtattatgcc tatatttgga gaccatggtc cagtagctaa agctgtattt
    25381 tcaataatct tctttgggtt aactttagca tttactatta ggtcttctaa ggttatagac
    25441 ataataggta agtatttaac tccagcgtta ttattagtat tatttgtttt aatagtaaag
    25501 ggtataattt ctccagcagg acctatgagt cctgagcata tgatagataa aaatctattt
    25561 ggtgaaggta tatcacaagg ttatcaaaca atggacgctc taggtgcagc atctatggca
    25621 actataatgt tgatgtctat aatagctaaa ggatatacat cagataaaga ccaaatagga
    25681 atgactatta aagctggttt tgtagcctgt gtatttctag cagtagtata tggtggtctt
    25741 acttatttag gtgcaacagt ttcaacttta tatgatacta gtatacctca agcatcttta
    25801 ttagttgaaa taactagtat attgttatat ggtcaagtag gaaaggtaat tttaggtgta
    25861 attgtggcac ttgcctgtct gactacttct acaggtttaa cagcatcaat atcaacttat
    25921 tttgaaggaa taagtaaggc gagttataaa caactggtta taggtatatg tgtagctagt
    25981 atgcttatat caaatttagg tgttgattct ataatagcca tatcagttcc aatcctacaa
    26041 actatatatc ctgttttatt agcaatagtt gttatggagt tggctggtaa acatataaag
    26101 aatgataatg cttttaaagg tgcagcatat gttacattag tgattagttt attatcagct
    26161 ataaatggaa tgacaggagc tgttccattt atacaaagct tgccacttgc aggtttgggc
    26221 tttaactggg taataccagc tatattgggt ggaatagttg gtaactttat aaaaagcaac
    26281 aaacaagtag cataataatt tgtaattaat ttaattttat aatatattaa tttttagtac
    26341 tatgggtagg tttaatctaa acctaatcta cccatagtac tttttagatt ggattataat
    26401 agcatgtata taatttttta taatataatt tttctttgaa gtatgataaa attagtaata
    26461 gtgataagaa tgtacaaatt taggaggttt taattaatgc ttagaaaaat aacagcattt
    26521 atgctatcca tatgtttaat tattggactt acaggttgta gtttacttgt aggcaagaaa
    26581 gataaagaga aaattataga tgattttaaa gttgcagtgg taactcaacc attatctgaa
    26641 aataaagtac aatacaatat ggtggaagag atggcaaaag aatatgagga agagaataaa
    26701 attgataaag acaaagatgg tcaaactaaa gttaaacaaa ctataaagca tgttgtattg
    26761 ccagagaatt ttacttctaa tatagacagt gctattaata agatagtaaa gttggctgac
    26821 gataaagaag ttcaggctat tgtagtttca actgaccaag cagggctttt acctgcttta
    26881 caaaaggtaa aggaaaagcg tccagaaata ataactattt ctgcccctat gggagatgat
    26941 aaaaaccaat taagccaatt tgttgatgtg aatctagggg ttagtgcaga ggaaagagga
    27001 aaagtattag ctgaacgttc aaaagaaatg ggagcaaaag cttttattca ttatgcctct
    27061 acagatgatt tgaaagatgt taatatagcc aaaagattag aaatgattaa agagacttgc
    27121 aaaaatatag gattgccatt tgtacaagtt aatactccaa atataaatac tgaagaagac
    27181 aaaaacaaag ttaaacagtt tttaaatgaa gatattgaaa aacaagttaa aaaatatgga
    27241 aaagatataa atgtatttgg tgtaaatgag tatatggatg aagttatatt gactaaggct
    27301 ttagaattaa agtatatagt tgcagagcaa agcaatccat caccaataca aacatatcct
    27361 agtgttatgg gtctaaaaat atctgaaaaa gatgctcaaa attatgataa aataaacgac
    27421 atgataagtg aaaaggctaa ggcttttggc atgtcaaata gacttggtgg atatcctatg
    27481 ccaatggatg cttttctacc atcagtagca atttatcttg ctactgaaat ggtgaaacaa
    27541 gatttaacac aagaagatgt atgtgaccca gattatttag aggcgtttac agaattaaga
    27601 tttggaatcg gttcagaatt tactccttta acagaggtac tttataatta tcaatcagta
    27661 attttaagcc aattaattta ttaatattaa ggaagtgtct caaaatgaac tttttagttt
    27721 atgaggcact ctttttctat taaatcaaat atatttttat ctttttaaca attaaaaaga
    27781 agcttatccc tatattgaga tagcttcgtt tttaatataa ataagtttta ttattttata
    27841 aaaaatttat agaaaaataa aaaattataa ttatatataa attttgataa agtaaagatg
    27901 aattactata ggtttaaatt atattatttt ctattatttt tatgcctttc tcagttattg
    27961 atgagccttt tcttccttta ctagaaatta agtatccatt gcgtgtcaaa tattctagca
    28021 tatttcttac ttcgtattct gtgacattat attccataaa tttcattaac tttgtcctcc
    28081 ccattggttt catgtgcaat gattttagga tagaaatgca tatgtttttg tcatattggt
    28141 ttaaatcatt gaatatggac atattacttg acatatgatt attatttaat tttgaagata
    28201 tgttttgagg taaatcattt attgtaagat aattggaacc catgtaagcc atatattcaa
    28261 tacaatttct cagttctcta atattcccca tccaagtgta tgaatttaaa gcttctaaaa
    28321 gactcttatc gagttttatg tttttatagg gaagttcttt taaaaatgat tctaaaagaa
    28381 gaggtatatc ttctaatctc tttctaagag gaggtaaatc taaagtgaac atacttatcc
    28441 tataaaataa atccatccta aatttatttt ctataattaa tttctctaaa tctttatttg
    28501 tggcagctaa tattctcaca tcaattggaa taatacttgt accaccaatt tttctaattt
    28561 ctttttcttg taagactctt aaaagtttaa cctgtaaatc atgtggcata tctcctatct
    28621 catctaaaaa tatagtgcca gtgtgagcta attcgaataa tccttttttt ccaccttttt
    28681 ttgctccagt aaaagaacca tcttcatatc caaatagttc actttcaagt aattcacttg
    28741 gtaatgaagc acaatttatt gctacaaaag gtttattttt tctatttgaa tgattgtgta
    28801 ttgactgagt aaaggcttct ttacctgttc ctgtttcacc tgttattaat ataggattat
    28861 ctgtaagagc catttttttt gcctttttaa tacaatcttt tataatagaa ctatttccaa
    28921 taatattatt aaatttatat ttagctacat aacctctttt gacagaatca cttcttattt
    28981 tttgttctaa tatttctata ttgtttacat ctttcaatgt aattaagcaa ccctctatat
    29041 ttttataaag ataaaatggt tttttagaaa gtatgaattt tttatctatt tcgtctatgt
    29101 atataatcat attatcgata tttaagttct ttgtaatttt atctaagaaa ctatttggca
    29161 tatattccat taaggaaggt gattttataa gtttttcatc tagtcccaac atattaagta
    29221 aagacttatt tacaaaagta accttgttaa aggtgttgaa aaatattaga ccatcaccta
    29281 tttcatcaat ggtcatgtaa agtatagttt ttaatttaga tatattatct aatgatgtat
    29341 ttaagaaatc atgacttagt gtctctttgg acaatttgta tagtttttct ataaatttct
    29401 cattttttag ttttaatact actaataatg acatatatgt atctaatgag atttttctcc
    29461 aacctaaatc aacaatattt tttatgtatt ttggtataaa tctaatctga cctggtgtta
    29521 tagctgtatc taaatcatat atttcatcac aatctggata tgtcggtaca aaatctatat
    29581 gatttatacc aattttatat agataagtag ccatctcaaa tgctgaagtt ttattggttg
    29641 tcataagtaa cgattttgta cctggaggta gtttcatcat tttttcatat ccaagttttg
    29701 aaagtgcttt cgttccatga ataatttcaa tatcttcctt aaaatgtttt attaaaaaat
    29761 tactagttaa aggtgataga gaaagcacta catctccctc taaattttct tttattccat
    29821 caagtatatt ttgtgtttct atgattattt cgtctccaaa aaagtttgtt aattcttctt
    29881 tgtagtggtc actattcaat ttatcatatg ttataaggat taacttcttt ttatccattt
    29941 tatttctcct aacaaaatag tatttatttt taaagtattt aaagtataag gcaagttata
    30001 ttatacaaca aatagtagta taaatttata tatttgatta aataataggt ttttttagga
    30061 ttttatccta taaaaaccta ttttaaaatt atttttgcaa gataaggaaa atagcttttt
    30121 atattgtaat gtatatttta aataattaat aaaaaaatat tatattttta aacttgaaaa
    30181 gtaagtaatt ataagctatt taatcactgg aataaattta caataaaaaa ataaatacaa
    30241 aggctttatc attaaggtat tggcataagt tttgcttatt aaacaagtat actaataaaa
    30301 ttcatttaaa caggagggag aatttatatg agtaaattag aatcaacaac agaaaaagtt
    30361 gaggtaaaat ggtatgggta tgtagcatta atattgggag cattattttt ttcaggaata
    30421 tttaaagatg caccaggacc attaaaagta ttggatttta acaatgtatt aggttctttt
    30481 ggaacattgg gaacaattaa tgatggtgta ggtacattag ctgcaaactt tagaggtgat
    30541 ggaggtactg gccctcgaga tggatggcta tatgcattga cattaatacc atcagttatg
    30601 tttgcacttg gagtagttag agttatagac cacttagatg gaatgaaggc agctcaaaag
    30661 cttcttagtc cactattaaa gccattatta gggttacctg gatttgcagg actaactttg
    30721 atagctagtt tacagtctac agatgcagct gcatctatga ctaaagaact taaagatgat
    30781 gggtatatag atgaaaaaca aaaagctgta ttttgtgcat ttcaattttc aggtgcgtct
    30841 gcaattacga atttctttgc ttctggagca gcactatttc catttatagg agatgtacca
    30901 atatttatac cacttgcttt aatattaatt atgaagtttg ttggggcgaa cttacttaga
    30961 ttatatttaa ataaatttga caagggggaa gcttaacatg agtgaggtag caaaaaaaga
    31021 agtaaaagtt aaaaaaggaa atgtgataga tgcgtttatt ggaggagcta gaaatggatt
    31081 ccaaataagt acaaattcaa tggctccaaa tgtcatcttc ggttttgcta taatttctgt
    31141 gttcaattta acaggtcttt tggatgtaat agggacaata tttacacctg taatgagtat
    31201 ctttggactt ccaggtgttg cagcaacagc tataatgaca atatttatat caatgggagg
    31261 agcatctgga gtaatcgcag gtttatttac agcaggtcaa ataagtcaag cacatgttgc
    31321 aattctattg ccaatgttat tgctttcagg agcgcagata caatttatgg ggagactttt
    31381 aggaacagct gagttaaaaa ctaaatatta tccacatatg tttgttatag caactttaaa
    31441 tggttgtatt gcaatgctta taatgaatta tttcgtagta taggtaatta taataaagat
    31501 ttaatataaa tggaaagttt aaattttaag gaggagctag attatggaaa ctaacaaaga
    31561 ctatattgag tatgtacata aagtgtatga ttatctacac actataccag aaaaaggatt
    31621 tgaagaatat aagacatcag agtttgtttt aaaagagctt gaaaaaatgg ggtttgaaaa
    31681 tattaataaa ggaattgcag gaactggtat tatagtaact atagattcta aaaaagaagg
    31741 accagtattt gctataagag cagatatgga tgcattggaa tttatcatag atggagaaaa
    31801 gaaaatgata catgcttgtg gtcatgatgg tcatacttct atgcttttaa cagctgcaaa
    31861 gttaataaaa gagagaaatt tagtaaaaag aggtaagtta aaaatacttt ttcaaccagg
    31921 tgaagaaaaa ttatatggag cattaagagt aattgattct ggtttgttgg atgatgtaga
    31981 agaaatggta gggatgcatt taagacctgg acaagattgt aggttgggaa aggcgatacc
    32041 tgcaatgtac cattgtgcta gctgtataat ggaatttacg attaaaggta tcacttgtca
    32101 tggttctaaa ccacatttag gagtaaatgc agtagatgct gtctgtttag cagtgaatgc
    32161 aataaatgca ataagagtaa atccagctat accaaattct ataaaagtaa caaatatatc
    32221 agttaaggga gaaacttata ataatatacc agatgaagca tttatggctt tagatataag
    32281 ggcagaaagt aatgatataa tagaagaatt aaaagaaaaa gcaatacatg ctgtaaaaac
    32341 tgctgtaagt tctataaatg cagaagctgt actaatttcc aaagatggag taccagcagc
    32401 agaatttgat gaagaacttg taaacttggc tgaagaaact ataaaagatg tattgggagc
    32461 tgatgcatct ataggtatat ttaaaaattc tggtggagaa gatttccatt attatacaca
    32521 aaaactaaaa tgtaaaacta catatattgg attaggagct gatgctacac caggatttca
    32581 taatccaaat gtaacatttg atacgaaagc tttagaatat ggtgtagata tatggtgtag
    32641 attggttgaa aagagattgg gataacatcc aaactaataa ttctacattt aaaattataa
    32701 ttatagaagt tatagaaaaa gattgcttat gttaattaga aaataattaa tatgagcaat
    32761 cttattttga tataatgaaa ctgattttgg aatattgaaa aaatatgatg ggaaagtaat
    32821 tatattttaa atctattgga caaaaaagtg atattattat aatattaaga attgtaaaat
    32881 aataccaaag tgcatctagt aatagactta acaattataa aaattttatt cttaggaggg
    32941 aaaaaatgag taagtataat tttgacagag taatagatag agtaggaaca gattgtgtaa
    33001 aatgggattt tagaactaat tgttcaacaa aagcacaaaa agatggtttg cctttttgga
    33061 ttgctgatat ggattttgaa tgtgcagaac caataataga agcattacgt aaaaaagttg
    33121 aacataaaat atttggatat agttcaaatg atagtgataa atactttaat gcagtttgtg
    33181 attggtataa aaggagattt aactgggaaa taaatagaaa agatattatt tttagtcctg
    33241 gtgttgtacc tgctgttgct attttagtaa gaatattaac taattctaat gaaggtgtaa
    33301 tagtccaaaa accagtgtat tacccatttg aagctaagat aaagagcaat aatagggatg
    33361 ttgtaaacaa tcctctgata tatgagaatg gtacttatag gatggattat gatgatttag
    33421 aagaaaaagc taagtgtagc aacaataaag tactgatact ttgtagtcct cacaaccctg
    33481 ttggaagagt ttggagagaa gatgaattaa aaaaggttgt agaaatatgt aaaaaatatg
    33541 acctatggat aatagctgat gagatacatt ctgatttaat tagaaaagga tttaaacata
    33601 ctcctttaca gtcattatgc ccagagtaca aggataaaat agttacatgt atagctccaa
    33661 gtaaaacatt taatctggca ggaatgcagt tgtctaatat aatagtgaat aatgatgaac
    33721 taaaaaagaa atatcaagaa gaggttacag ctgttggagt tgcaacttca ccaaatccct
    33781 ttgctattgt agctacaata gcagcatata atgaaagtga agactggctt aatgaattaa
    33841 atgattactt agataataat atacaattta ttgatgaata cttaaaagaa aacctaccaa
    33901 aagtaaaact tgtttatcca gaaggaacat atctagcatg gttagatttt actgcttatg
    33961 gattaaatga agtagaactt gaggatttaa tgtttaaaaa agctaatgta ttatttgatg
    34021 aaggttatat atttggaaaa gaaggaatag gatttgaaag aataaatgta gcttgtccac
    34081 agtctttatt aaaagaatgt atggacagat taaagactat atttgaaaat ttataaagta
    34141 taggtaaaaa tttataaagt gtttaaaaat aaaaaaataa taaacttgac aaattttaac
    34201 taatcactta tcatactatt atgaataaat agaaaacata agagagctaa attttattga
    34261 attaaactct taaagacttt gaaaggaaga gtagttatag aatgtaatta aagcgagttg
    34321 gtgatggtgt gagaccaata tgaagtctat aatgaagaac atcctggagt tgctaaccga
    34381 aattttaaaa gagtaggctt agtcggaacc aaaccgttat catattgggg tgtatcgagt
    34441 aatatctgta cattgtgagc tgggtttgaa tttatttcaa actaatcagg gtggtaccgc
    34501 ggaaaagcaa cctttcgtcc cttttatata gggatgaagg gtttttttaa tggaaaaata
    34561 tataaatata gggagtgaat gatatgagca caataatacc aaaggattat aaaagtagtt
    34621 tgaacgtaat tgatacacaa atagctataa aaaaattaaa agattttttt gaaatgagac
    34681 taagtaatga attaaatctt ataagagttt cttctccgtt atttgtattg ccagaaacag
    34741 gtgcaaatga taatttaaat ggagaaaagg ctgtaagctt tgatatacca tttatgaata
    34801 aaaatgcaga aattgttcaa tcacttgcaa aatggaaaag attagccctt aaaaaatatg
    34861 ggtttgaagt tggtagtggt ttatatacag atatgaatgc aataagaaaa aatgaagaat
    34921 tagacaatat acattctctt tatgtagacc aatgggattg ggaattggtt atagataaaa
    34981 gtagtagaaa tgaaaagact ctaaaagatg ttgtaaaaag attgtatgga gtatttaaag
    35041 atactgaaat ttttgtatgt agtatgtatg aaggtataaa agaaatatta ccagaagaaa
    35101 taacttttat aacatcacaa gagttagaag atatgtatcc agaattatca ccaaaggaaa
    35161 gagaagataa aattgtaaaa gagaagaaag cagtatttat aactcaaata ggaaaaacat
    35221 taatatcagg agaaaaacat gatggaagaa gtcctgatta tgatgattgg gaattgaatg
    35281 gggatatatt attttacaat cctgttttag attcagcatt agaattatct tcaatgggaa
    35341 ttagagttga tgaagaatct cttgatagac aattgaaact tgctggatgt gaagagagaa
    35401 aagagtttga ctatcataag atgcttttaa atggcgaact tccgtataca attggtggag
    35461 gaattggtca gtcaagaata tgtatgtatt tcttacaaaa agctcatata ggagaagtac
    35521 aagttggagt atggccacag gatatgattc aaaattgtag tagtgcagga attgaattat
    35581 tataatttaa atataaacta attgataaaa tttatatcta atatgttcat caaattaatt
    35641 atatcggtta taatgtagaa taacctataa ttataagagg tgataaatat ggagttttta
    35701 aaaataatca ttaatatagt tttagatatt ttaaaaaaaa ttttggtcag atttaaaaat
    35761 gctaaatttg gtttggtttt tgtatttgat ttacttaaac tacctgattt tatgacagat
    35821 aaaagaatca atatagttga taaaataaag gtaatttctg tattaatttt tactatatcc
    35881 tactttgtat ctggggttga tataattcca gagatgattg caggggcatt tggttttata
    35941 gatgatgcta tagttttaat ttggagtata ggtatagtga atgaagaaat taataagtat
    36001 agagttatca caaaaaaaga taaacattca aatataattg aaaatgtaga attttctata
    36061 aaagatgaag aagaataaca atttatatat gttattcttt tttttattaa caaaaaatgg
    36121 ttaaattgtt gacggagagg taaaactcta ttatcataat aagtggatat ccaatattta
    36181 actaagtatt caatgtgcaa gaatgcttaa tttaaaaaag ttatggaggg taaatattat
    36241 gaagaaaaaa ataggtttag tgcccaaact aatcatcggt atcatagtcg gtattttaat
    36301 cggaagttat gcaccagaaa taatagtaca aatattagta acagttagta ctttattctc
    36361 agcattccta aaatttgtta taccatttat aattataggt tttgtaacag caggtatagc
    36421 tgatttagca acaggtgcag gaaagttatt ggggataact acgggaatag catatggttc
    36481 taccttaata gcaggattat tatcatttgc agtatctaca ttaatcttcc caaattttat
    36541 tgatgcaagt gttgcaagtc aaattggaga cccagaggca ggaatgttag ctccaatatt
    36601 cacaattcca ttagagccta tggtagatgt aacagctgca atcgtatttg catttgtaat
    36661 gggacttgga atatcagctt taagaaatca tggaaaagga gagactttat ttaatttctt
    36721 ccaagaattt caaggaatcg taacgaaaac gttgtcaaca gtgattatac ctttattacc
    36781 tttatacata gcaggaactt ttgcaaatat aactatagca ggggaagtat ggacaatatt
    36841 aggggtattc tggaaagtat acttagtagt aataccttta cattttgtat atatggcatg
    36901 tcaattcaca gcagcaggtg tattttcagg aaaaaatcca gtaagaatgt taaaaaatca
    36961 agtacctgga tatttaacag ctataggaac tcaatcttca gcagcaacta taccagttaa
    37021 tgttggttgt gcagaaaaaa atggagtaag taagcaaata agagaatttg ttgtacctct
    37081 ttgtgcaaca atacatttat caggtagtat gatatcaata acaggattta caactgcagt
    37141 tttaatgatg aatggtatgc cacatggaat aggtgtatta ttcccttata ttgcaatgtt
    37201 aggtatagca atggttgcag caccaggagc accaggtggg gctataatga gtgcacttcc
    37261 tttcctacca atggttggaa taccatcaga tggtgggctt gcaagtttaa tgatagcttt
    37321 atacttaact caagacagtt ttggtacagc atgtaatgta tcaggagata atgcaatagc
    37381 agtagtagta gataaaataa atgatggaat gagtaaaaaa ggaaacaaag aagagcaaag
    37441 tgcttagttt tttaataatc aaaaatgagt tatctcaaat agagatagct cattttttat
    37501 ggtataaaaa attataaaat aaagtgattt ttttatttaa aaattgcttt aagagatgat
    37561 attaataatt tattttcatt aggttttaat atacatactc taaagaaata ctcatctaga
    37621 ccttcaaatg aacaacaatc tcttattatt attttttgtg ggagcagttg ctctcgaaga
    37681 gatttagcag tcaattcctt tgtcttgatt ttacacaata tgaaattacc tttagtattg
    37741 tatatatcta agctttttat attcttaagc tctttaagca ggtaatctct ttcagtgttc
    37801 attaaggaaa tagtatttga tataaaatct aaatcactaa acatgatttc acccatttta
    37861 gaagctatta tgtttatatt ccataaatct agatttttat ttatttcatt tttgacatta
    37921 gtgttagata taagaccata tccaagcctt attccaggcg ttgaaaaaaa ttttgatgta
    37981 cctcttataa cgaataaatt agaataatca tcaactaatt gagtacaaga gtatgtatct
    38041 gtatcagtaa actctacata agtctcatca atcattaaaa atgagtctgt attttttaaa
    38101 atttctctca cttctacttt agtaaatgca aatccagttg gattactagg attacagatt
    38161 actaccaaat cataatcttt agcattgata gtttttataa gattttctaa atttatatga
    38221 aaattatctt cttcttttgc aaagtatttt tctatagaac aattaatttt agataactct
    38281 ttttcatact ctgaataggc tggagagagt aaaagagctt gtttaggatt aattgtatgt
    38341 ataaatgatg aaattagttc tgtagctcca ctgccaagta ctatattatc tatagaacat
    38401 ttgcagtaat tggatataga agtttttaag tctatgtaat cagggtcagg atacatagat
    38461 accatatcta tattgtttac aatgtattgt ttggctaaac ttgatgtacc aaaaggattt
    38521 atatttgagc taaaatccat aaattcttcc tttgaaaagc catacttact agataaacta
    38581 tataaattag ctccatggtt tgtgctcata aaagtacctc caaaaataaa attatataat
    38641 cgataaaaat aactgattaa tatatgtaca tatagattat aatacaaagg aatcgttatt
    38701 ccaaattttt aatgtaaaaa gcaaaaaagt atggataagc tgttaattta ggtttaaaaa
    38761 tgatgaaaaa gattttaatt gatattttgg tatgctataa tatataagtt aatagaagga
    38821 aagcgggagg atacaaaatg tacaaagtta caggaaaaat gttgacagaa gaacaaataa
    38881 aagaaaaagt ttatgaatta ggaaaaaaga tagaagaaga ctttaaaggc gaagatttac
    38941 tagtggtagg tatacttaaa ggagctagtg tatttgtttc agatttaata agatgtatag
    39001 atttggatgt aaatatagat tttatgagtg ttacaagtta tggaaactca acagagtctt
    39061 ctggaacagt taaaatatta aaagatttag atgtagatat tgaaggaaag aatgtgttga
    39121 ttgtagagga tataatagat tctggtttaa cattaagtaa tctagttgca gctttaaaaa
    39181 ctagaaatcc aaaatcttta aaactatgta ctctacttga caaacctcag agaagaaaag
    39241 ctaatatacc tgtagactat gttggatttg ttatagaaga caaatttatt gttggttatg
    39301 gaatagacta tgctgaaaag tatagaaatt taccttatat aggtatagtt gaggatgtag
    39361 aatagattta aatgtgaatc aaaagcacta atttctaagg gagttagtgc tttttagtta
    39421 attatataaa attttagtaa aggagacatc tatatggata ttcagttaaa tatattatta
    39481 tttttccaaa atttaagaaa tccaattttg aactttatat ttcttgtctt tacaataagc
    39541 acagaagcac cgttattaat attaattaca acaattatat actggtgtat aaataaaaaa
    39601 tgtggacaga aaatactgtt tgctataatt ggaaattttg ttgtaaattt aggcatcaaa
    39661 gagtttgtaa aagctcccag accaatagga ataaaaggtc ttgaatcatt gagagtttct
    39721 actgctggtg gttattcttt tccaagtgct catactcaaa ctgcaacaac attttgggtt
    39781 agtattatga ctatatttaa aaagagatat cttcatttaa tagctgcagt tatggtttta
    39841 ggagtaggat tgtctaggct gtatttagct gttcattggc caatagatgt gatagcgggt
    39901 tggatattag gaatattttt tacagttgta tttgtaaaaa tatttgacta tattgatgat
    39961 aataaaaatt atattttact tgtagtttta cttataccat ttataatagt ggctatattt
    40021 ctaaatagcc ctgaatattt tgaaaaattt ggtataatag taggatttgt atttggatat
    40081 atggttgagg atagatttgt taaatttaat acagataata ataacaaaaa aataaatttt
    40141 agtaataaaa ataaggggag taaaaatata attttttcaa gaatatgtag gtttttggtt
    40201 gggatagtaa caataggaat gttatatatt ggcattaagt tgtttatttc aatgttaata
    40261 gtcactttaa atatgagtga ctctactatg agtataattt ttatgaattt tattaagaat
    40321 acagtagttg ttttttatgg aatagcagga gctccagcat tatttaaact tttaaaatta
    40381 aattgatttt aaggtataga gtcaatagtt taaattaaaa aaattaaggg tagaaaagag
    40441 tgattatttg aagaaggtag taataatagg tggtggacca gcagggatga tagccgcatc
    40501 aactgcatgt gagaaaggct atgatgtaac attaattgag aaaaatcata aattaggaaa
    40561 aaaacttgca ataacaggaa aaggtagatg taatattact aacgcttgtg aaatagagga
    40621 attaatagaa aatgtaccta caaatggaaa atttctttat agtgcttttt atacttttac
    40681 aaatgatgat gtaataagta tgtttaataa tcttggagta aagactaaaa cagaaagagg
    40741 aaaaagggtt tttccagaga gtgataaagc atttgatatt gtaaatgctc ttgaaagaca
    40801 acttaaaagt aagaaagtaa atatattatt aaattcgaaa gtagaaaaaa taatatctaa
    40861 aaataataaa attgaaaaag taattttaaa cgataaaaaa gaaattaagt gtgattcagt
    40921 tgtagtagca actggtggat taagttatcc acttactggt tctactggtg atggatataa
    40981 atttgcaatt tcacaggggc acacaattat agatacaaaa ccatctttaa ttggtataga
    41041 agtacaagaa agttttacaa aagatttaga aaaattatca ttgagaaatg ttgaaataag
    41101 ggtgtttaat agcaaacaaa agaaagttta tagtgatttt ggagaacttg aatttactag
    41161 atttggttta gatggaccta taatcaagtc tgcaagttgt agaatgaaag atacaaggaa
    41221 agaaaattac actatattat tagatttaaa acctgcatta gatgaggaaa aattggataa
    41281 aagagttcaa aaagattttc agaagtatac aaataaaaaa tttgaaaaag cattagatga
    41341 tttacttcca aaaaaactaa tacctataat aataaactta agtgaaatta atccaaatac
    41401 ggttgttcac caaatttcta gagagcagag aaaaaactta gtccatttat taaaaaattt
    41461 aaagtttaca gtaaagagat acagacctat tgaagaagct ataataactt ctggtggtgt
    41521 aaaagtaaac gaaataaatt caagtacaat ggaatcaaaa ttagtagaag gtttattttt
    41581 tgctggagaa gtaatagata tagatgcata tacaggtgga ttcaatttgc aaatagcttt
    41641 ttctactgga tatttggcag gttttaattg ctaaaaatta cctgaattaa aaatgtttaa
    41701 aatcacataa tattatcagc aacacaaaat aaattaaaca gaaggaaggt aatcaacatg
    41761 gcaagtaaca ataacaacaa cagaacagta gtaccaggag caaaagaggc tttaaaccaa
    41821 atgaaattag aaatagctaa tgaacttggt atgagtaatt atcaacaagt tgataaagga
    41881 aaccttactg caagagaaaa tggttatgta ggtggataca tgactaaaaa attagttgaa
    41941 atggctgaac aacaaatggc tggaaaaagc aacagatagt tataaataat tatttataac
    42001 tattggctat tagtttagct agagctatct caaatataga aagtaatttc taaattgaga
    42061 tagctttttt ttattaaatt attaaataga tatattttta tgtaataaat tttatgtatt
    42121 tggataaaat aatacaaatg aatatgttac ataaggagtg atgtaaaatg agtagtgtaa
    42181 attttgagga tttaaaaaac aaatttatta atagtgactt agatgaaaaa ataagaatat
    42241 atacaacaac agaaggttta tcagtagaac aatttaaaga gctactaaaa tattatccaa
    42301 tacaacattt atctaaacta gaaaaagcac ttggatagca gtatttaatc caaaattaaa
    42361 aaggcaattt atttaatatt aaaaatattt tttattaaat aaattgcctt tttataatag
    42421 gtttaaatta ttatgctttt gatatataat tatagattct ctcatttcca agttcaatag
    42481 acccagtatt aataagagta actattcctt ctatatctct agcagttaag tatttatatg
    42541 gaattaataa attataacca gtattactta gtaagttttt taattgggtt atttgagaga
    42601 tattgaaaca ttttgcagtt ttagtagatt tttttcttaa gttagataaa ttttcatttt
    42661 gaatatttat taaagtttct atatagtttc tataagatat cttaaagttt gtctcattga
    42721 ctattttttc attaagtata ctactgataa aatcttgaga caatatatta tctgcaaatt
    42781 tatctgctgt taattttcca gatgttatgc ctttttgata ttttctaaga atttttttag
    42841 tatagcagta tattttattt ctcgtttcta aatcgagttg ttctatttgc acaaaaccac
    42901 ttccttctaa ttataaaaat atttattaaa aatctatact taactaataa agcttcaata
    42961 acttatttac aataaatact aaatcataaa atatttgtta ttttttatta ttaccaagta
    43021 taaaaaatat tataattgta aaacttacaa atatatgtaa aatatttagg agtgatgaaa
    43081 atgttaaatg ggataataaa gcttttttat tctgaaattg taaaaacaaa gattaataac
    43141 ttagattcaa gtggatattc tttaagagat gaatgtaaag aaatcagaaa tataactcta
    43201 aagatagata gtgtattttt taagctttta aagttagctt ttggttcaat attgcttatg
    43261 gtagctttta atttaaacat ggttttaggt ataggagtgt ttttagttgg tgtagtttat
    43321 atactatata aaagaaatat ggaagaacaa gtaaaagagc atataaataa tgtgaaaaat
    43381 aatatcgaca tctctaaaat aacttttatg gatgaaaaag ggagaacagg tctaaatgca
    43441 ttaattacat tacttcttat aggattattg acaggattta actggatatt agttgctagt
    43501 tttgtcgttg tttttgtttt tactataaag aatgtatgtt aaaatactaa taaatagttt
    43561 aatatatagg gggagaaatt gagtatgact aatggcagtt ataaaatgaa gaatttagtg
    43621 gaaatatccg ctatggtgac tgaatctaca gattttttta atataaaaga tgacataata
    43681 gaaaaaatgt tagaagttgt tcaccctaca aaggcatgtg tgaatctatt ttataaaaat
    43741 gattctaaat atgcctactt agtatgttcg caaaccttag aatatgtacc tcaaattttt
    43801 ccaataaata gtcttaaagg tgcaaaaatt gattttgaca catatccaga gtatatacat
    43861 gaggctgtaa atgaaaaaaa gataatatat gtggaaaatg tatttgaaga ttctagagca
    43921 gaaggtgaac gagatttagc aaaagctgaa ggttatattg ggagaatagt ttttcctttt
    43981 ataataaata acgtagttgt tggatttatg acttgttttt taaaagagga tgattatata
    44041 acagaacaag atatagattt tatatcatct gttgcatcac tcttaagttt atcaatagaa
    44101 ataactaata aaaataataa tgtacaaatt ttaattgata aactaagagg ctcaataagt
    44161 gccataaatg aagctactaa aaaactatac cttaataaga gtattgatgg atttttggaa
    44221 aatttaagta aacaagcgtg taatattact aatagtaaag aagctttaat aattataaat
    44281 agtcatcata ataaggatgc tatagttagt tgttataatg ttgagcaaaa aaagaaaaca
    44341 aatttatatc ctatgattga tatgttttta aagaaagagt cattgggggg gtatttaaat
    44401 aatttaaaac catgctcaaa aaagggaata aatttggaaa gttatatata ttacaaactt
    44461 caggataaaa atgatactat aggatgtata gtttgtgcaa atagcaaaaa ctatactagt
    44521 gatgatttaa atattcttag tatattgtcc aaacaagttt ctgttggtat gcaattatat
    44581 gaacataatg aaaaggaagt aaaacacaaa gttcttgaaa atgagttgaa catattaaat
    44641 aaacaacaac agcttattat ggataaaggt aaaatggatt gtaatgatga gaaagaactt
    44701 tatttttacc ataaaccagc aagggtagta ggtggagatt tttattatgc tacaaaagtg
    44761 gatgacgaac atatagttta tattgttgct gatgtaatgg gacatggaat ggtatcaaat
    44821 tatgttgttg caatgataaa aggtgctttt aaagtattat gcaatcaata caatacacca
    44881 agagaaataa tgacaaattt aaataggatg ttatatgatg aatttgacaa gatgggagta
    44941 tttacaactt gtttgatagg tattatagat actaaagaaa atatgattac tgtgtctaat
    45001 gcaggtcact atagtcctgt agttgtaaag aaagatggca caatagaaaa taatttaaat
    45061 tgtaaaaagg gcatacctat tggaataatg gaagatgcta cttatgaaaa taatactctt
    45121 tcaatggaag attatgcaat ggtatgtatg tacactgatg gaattttaga gataaaaaat
    45181 tcatcaaaag aagagtatgg tataaaccga ttagaaaact ttctaaaaga aaattttaga
    45241 ttaaatcaac agttaatagt agaaaatcta aaattggatt taaagaactt ctcatcaaaa
    45301 gataattatg atgatgatat tttaatagtt atgttaaagg ataggtagat tgattatgga
    45361 ttcaaacgat aaaaaaggac ttaaaagtcc agagattata ataataaatg gaagtccaag
    45421 aattaataaa aattgcagtt ttatagcaaa tgaagtaaaa gctatgctag aagagtgtgg
    45481 attaagttgt aaaatattta atatctacaa tatggatatt gaatattgca cagcatgtgg
    45541 attttgtgaa aaaacagggt attgcaagtt taatgatgat atgaagccat tatataaaat
    45601 gtttgatgat tcaaaaggaa ctatagtaat aagtcctgta cattttgatt caatatcagc
    45661 taaattaaag actgttgttg atagaactca agctatatat gcaagtaaat atgtattaaa
    45721 taaaccttca attgatagaa gtaagaaacg gataggaatg tatattgcag tgggagggag
    45781 taaatcatat gattcacagt ttaaaggtgg acagattgtt atggattttt tctttaagag
    45841 tataaatact aaacttttac acaatatata tttaaacaat acagatgatg tatcttatat
    45901 agataatcaa gaatttaaag atataatata taaaaattta aagatatata ttgattcaat
    45961 ataatttgat tttgtaggta atattatgta taattgtatt gcaataattt aaataattaa
    46021 aaggagggaa tttcaatgaa attaccaata gcattatcta ataaacatgt tcatttgagt
    46081 caagctgata tagacgtatt atttggagca ggtcatgagt taactaaatt taaaccatta
    46141 tctcaaccag ggcaatatgc ctgtgaagaa aaagtggatt tagtaggacc aaaaggaact
    46201 ttaaaaggag ttagagtact aggaccagct agagctcata ctcaagttga aatatcttta
    46261 gcagatggtt tcccattagg agtaaaagca ccaataaaag aatctggtga tatagctgga
    46321 actccaggtg ttaaattagt aggacctgct ggagaagttg aaatgaaaga aggagttata
    46381 gttgcttcta gacatataca tatgagcaat gaagatgctg ctaaatttgg tgtaactgat
    46441 aagcaaatag taaaagttaa aacatctgga ccaagagcat tagtatttga aaatgtatta
    46501 gtaagagcta gtgacagctt taatctagaa atgcatgttg atatggaaga aggaaatgca
    46561 gcaggcgtta aaaatggaga tttagtagaa ttaatagttg attaatatta taaatagact
    46621 ttatgctagt agatatttta tttgctagca tatttttaaa tgtaataaat ttctatagga
    46681 taaatttaat cgattagatt aatttataaa ataaaggggt attataaaaa atgtaataaa
    46741 aacttacaaa aatatgatgt aatatggtaa aattatatat ataaaaattt acatataata
    46801 aaga
//
LOCUS       DAENCV010000017        45610 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAENCV010000017 DAENCV010000000
VERSION     DAENCV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.017"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            166..549
                     /locus_tag="KQD82_000905"
     CDS             166..549
                     /locus_tag="KQD82_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG4379389.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            551..901
                     /locus_tag="KQD82_000906"
     CDS             551..901
                     /locus_tag="KQD82_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379390.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1002..1460)
                     /locus_tag="KQD82_000907"
     CDS             complement(1002..1460)
                     /locus_tag="KQD82_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379391.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEERFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1505..2293)
                     /locus_tag="KQD82_000908"
     CDS             complement(1505..2293)
                     /locus_tag="KQD82_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4379392.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTTVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2432..2869)
                     /locus_tag="KQD82_000909"
     CDS             complement(2432..2869)
                     /locus_tag="KQD82_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG4379393.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3691..4200
                     /locus_tag="KQD82_000910"
     CDS             3691..4200
                     /locus_tag="KQD82_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379394.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4598..4804
                     /locus_tag="KQD82_000911"
     CDS             4598..4804
                     /locus_tag="KQD82_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HBG4379395.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTKLSLCYLTNGAQK"
     gene            complement(5098..5898)
                     /locus_tag="KQD82_000912"
     CDS             complement(5098..5898)
                     /locus_tag="KQD82_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379396.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6093..7691)
                     /locus_tag="KQD82_000913"
     CDS             complement(6093..7691)
                     /locus_tag="KQD82_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG4379397.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8019..8195
                     /locus_tag="KQD82_000914"
     CDS             8019..8195
                     /locus_tag="KQD82_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379398.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8369..9499
                     /locus_tag="KQD82_000915"
     CDS             8369..9499
                     /locus_tag="KQD82_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG4379399.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            9740..10090
                     /locus_tag="KQD82_000916"
     CDS             9740..10090
                     /locus_tag="KQD82_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG4379400.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10098..10547
                     /locus_tag="KQD82_000917"
     CDS             10098..10547
                     /locus_tag="KQD82_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG4379401.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            10649..11224
                     /locus_tag="KQD82_000918"
     CDS             10649..11224
                     /locus_tag="KQD82_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG4379402.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11217..12626
                     /locus_tag="KQD82_000919"
     CDS             11217..12626
                     /locus_tag="KQD82_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG4379403.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            12810..14243
                     /gene="eutA"
                     /locus_tag="KQD82_000920"
     CDS             12810..14243
                     /gene="eutA"
                     /locus_tag="KQD82_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG4379404.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPVVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14261..15625
                     /locus_tag="KQD82_000921"
     CDS             14261..15625
                     /locus_tag="KQD82_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG4379405.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            15638..16519
                     /gene="eutC"
                     /locus_tag="KQD82_000922"
     CDS             15638..16519
                     /gene="eutC"
                     /locus_tag="KQD82_000922"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG4379406.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            16539..17192
                     /gene="eutL"
                     /locus_tag="KQD82_000923"
     CDS             16539..17192
                     /gene="eutL"
                     /locus_tag="KQD82_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG4379407.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17203..17904
                     /locus_tag="KQD82_000924"
     CDS             17203..17904
                     /locus_tag="KQD82_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG4379408.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            17907..19376
                     /locus_tag="KQD82_000925"
     CDS             17907..19376
                     /locus_tag="KQD82_000925"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG4379409.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYKTLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19472..19759
                     /gene="eutM"
                     /locus_tag="KQD82_000926"
     CDS             19472..19759
                     /gene="eutM"
                     /locus_tag="KQD82_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG4379410.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            19886..20647
                     /locus_tag="KQD82_000927"
     CDS             19886..20647
                     /locus_tag="KQD82_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG4379411.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            20660..21289
                     /locus_tag="KQD82_000928"
     CDS             20660..21289
                     /locus_tag="KQD82_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG4379412.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21331..22056
                     /locus_tag="KQD82_000929"
     CDS             21331..22056
                     /locus_tag="KQD82_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG4379413.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            22069..22341
                     /locus_tag="KQD82_000930"
     CDS             22069..22341
                     /locus_tag="KQD82_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG4379414.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22334..22885
                     /locus_tag="KQD82_000931"
     CDS             22334..22885
                     /locus_tag="KQD82_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG4379415.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            22897..23988
                     /gene="eutH"
                     /locus_tag="KQD82_000932"
     CDS             22897..23988
                     /gene="eutH"
                     /locus_tag="KQD82_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG4379416.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            23990..24463
                     /locus_tag="KQD82_000933"
     CDS             23990..24463
                     /locus_tag="KQD82_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG4379417.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(24583..24684)
                     /locus_tag="KQD82_000934"
                     /pseudo
     CDS             complement(24583..24684)
                     /locus_tag="KQD82_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            24979..25500
                     /locus_tag="KQD82_000935"
     CDS             24979..25500
                     /locus_tag="KQD82_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4379418.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            25879..27054
                     /locus_tag="KQD82_000936"
     CDS             25879..27054
                     /locus_tag="KQD82_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4379419.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            27088..28626
                     /locus_tag="KQD82_000937"
     CDS             27088..28626
                     /locus_tag="KQD82_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG4379420.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            28658..29860
                     /locus_tag="KQD82_000938"
     CDS             28658..29860
                     /locus_tag="KQD82_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4379421.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDSHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGEGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(30027..30626)
                     /locus_tag="KQD82_000939"
     CDS             complement(30027..30626)
                     /locus_tag="KQD82_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4379422.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            30796..32154
                     /locus_tag="KQD82_000940"
     CDS             30796..32154
                     /locus_tag="KQD82_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379423.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEKLFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            32147..32779
                     /locus_tag="KQD82_000941"
     CDS             32147..32779
                     /locus_tag="KQD82_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG4379424.1"
                     /translation="MNNKYIRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDIDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            33108..34265
                     /locus_tag="KQD82_000942"
     CDS             33108..34265
                     /locus_tag="KQD82_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4379425.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(34412..35047)
                     /gene="lexA"
                     /locus_tag="KQD82_000943"
     CDS             complement(34412..35047)
                     /gene="lexA"
                     /locus_tag="KQD82_000943"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG4379426.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(35151..36278)
                     /locus_tag="KQD82_000944"
     CDS             complement(35151..36278)
                     /locus_tag="KQD82_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4379427.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(36599..37444)
                     /locus_tag="KQD82_000945"
     CDS             complement(36599..37444)
                     /locus_tag="KQD82_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379428.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNINLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(37474..37845)
                     /locus_tag="KQD82_000946"
     CDS             complement(37474..37845)
                     /locus_tag="KQD82_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG4379429.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(37998..38258)
                     /locus_tag="KQD82_000947"
     CDS             complement(37998..38258)
                     /locus_tag="KQD82_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG4379430.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(38447..39391)
                     /locus_tag="KQD82_000948"
     CDS             complement(38447..39391)
                     /locus_tag="KQD82_000948"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG4379431.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(39412..40266)
                     /locus_tag="KQD82_000949"
     CDS             complement(39412..40266)
                     /locus_tag="KQD82_000949"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG4379432.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(40281..41642)
                     /gene="accC"
                     /locus_tag="KQD82_000950"
     CDS             complement(40281..41642)
                     /gene="accC"
                     /locus_tag="KQD82_000950"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG4379433.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(41658..42110)
                     /gene="accB"
                     /locus_tag="KQD82_000951"
     CDS             complement(41658..42110)
                     /gene="accB"
                     /locus_tag="KQD82_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG4379434.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(42380..42826)
                     /locus_tag="KQD82_000952"
     CDS             complement(42380..42826)
                     /locus_tag="KQD82_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379435.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            43101..43379
                     /locus_tag="KQD82_000953"
     CDS             43101..43379
                     /locus_tag="KQD82_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379436.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(43508..44254)
                     /locus_tag="KQD82_000954"
     CDS             complement(43508..44254)
                     /locus_tag="KQD82_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379437.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(44383..45027)
                     /gene="trmB"
                     /locus_tag="KQD82_000955"
     CDS             complement(44383..45027)
                     /gene="trmB"
                     /locus_tag="KQD82_000955"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG4379438.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(45165..45485)
                     /locus_tag="KQD82_000956"
     CDS             complement(45165..45485)
                     /locus_tag="KQD82_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG4379439.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        17240 a         6106 c         6962 g        15302 t
ORIGIN      
        1 aaagaaaaat atatagaaaa tccttataaa aatttaaatt gtaatgaata ggaagtaatt
       61 aaaaagaatt tttattgatt tttaaaaatt tagttagaaa ctatatcatt atggtaaaaa
      121 atgtattata atgtttagaa atattataat aaggagcgat taaacatgaa tctatcgaag
      181 aaaatagaag ataaaagaaa agctaaacag agagctgaaa aagttaagaa agctaaaatt
      241 gctacagctg gtgtggtatt aggagctgtt actggtgcag tgagtggagt acttttggct
      301 cctaaatctg gaaaagaaac aagagaggat ataaaagatg cttcacaaca aattgctgaa
      361 aagataaata tgaaaactgt agacgtaaaa ggcaaagtat ctgagaagct agaggataaa
      421 aaaggaaact ttatagaatc aaagaaaaaa ataaaaaaat atatagataa caaaaagagt
      481 gttgaaaatt ctagtgagga agataaaata atagatatag ttgaaccagt tagtgttact
      541 gaggaataat atggacaata caataacgat taacataagt ataatatatg tattattagc
      601 tatacttgct tgtattgctt tagtttattt aattattgta cttgttaaac tacataagat
      661 tttaaataat gttggaacta tgttaaatga aaatactcaa aatataaata caactttaaa
      721 taatcttcct aatgttgtag aaacttttgg agaagtagga gaaaatatga aagatgtaac
      781 agaagtagtt actgaagtta cagcagattt tcttgttact aaagacagtg tcaaatcaaa
      841 tatggatgtt atagttgata tattgactat aattaaaaac atttttctag ataaaaaata
      901 ataagttaat ataaaataag cctataagat ataatttaaa cgaccttaaa atagcattta
      961 taaggtcgtt ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc
     1021 ctctttatca tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca
     1081 tctttacatt caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa
     1141 aagatataag aattatatag ccattcttca aatctttctt caagtatatc ccaatacgaa
     1201 ctccaaaata atccacaacc tgatatataa gatgctctac tttgatttat ataaacctca
     1261 tttagaagct tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt
     1321 aattcattta aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta
     1381 taattttcat caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct
     1441 acaacttttg ttcttatcat ttttttatcc taacttgttt attttttata aaataatcat
     1501 gattttataa tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac
     1561 catttattac tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct
     1621 ttttacctaa accttgctta taactagacg cctcaattat taaagaatct ttcaattgcc
     1681 aatgtaaaat ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat
     1741 acactttatt ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg
     1801 ctatcaattt agcaacttta tctttattat taacataata atctgtatct gctttactat
     1861 ctacaaaaca tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac
     1921 ctctaacatc agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc
     1981 tttctgcata ggttttgcca ttattagtct tatatattgt ttctgtccct actgtagttg
     2041 aagttgtagc atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg
     2101 ctatagcaca ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag
     2161 tagctccacc tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc
     2221 tttcgtttat atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta
     2281 ttactacttt caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc
     2341 ataacctaga attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa
     2401 aatatagaat attaagtaaa taatgttaaa attataattt tgtatataag acattacttg
     2461 attgcaataa ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt
     2521 taattccagc ttgaattata gccttagtac aatttaagca aggaaagtga gttacataaa
     2581 tagagcaatt tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag
     2641 cgtgaatggt tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct
     2701 tatctccaga aatactacca ttatatccag tagacactat tctgttttct gaattgacaa
     2761 ttatagctcc tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc
     2821 tcataaaata ttcttgccat gagcatctat tttcaagttt tttattcaaa tcatatacct
     2881 ccaaaattta tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt
     2941 atacgataaa taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa
     3001 agttacaagg aatctaaaat tatatatatc tttcttcgta atatgaatgt tacaatataa
     3061 aaaactgtct caataaaata gagacagttt tacagtcaaa gtttataata caagttcata
     3121 tttttcttta aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc
     3181 tttagctatt ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt
     3241 taatctccct tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg
     3301 ctgcatatac aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct
     3361 ttaaccgaca tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt
     3421 tttatttttt tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata
     3481 aatatttcta cagttttaat tactggacat atctattttt attataaaaa gcgcttaaaa
     3541 atcgagaata tccgcataaa aactatatat aagcaaaacg attaaaggaa aaagaagaaa
     3601 tttacttgaa tgttaaaatg cgttgcttga agcaatgggc agttttgctt ataatatcgg
     3661 taacaactga aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg
     3721 aagtcaaaag gactttcaca acaagaactt gctataaaac tgaatgtagt gcgacaaaca
     3781 atatctaaat gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca
     3841 gaggtgcttg aaacgcccgt aagcacttta cttggagaaa ctattgttga gtcggaagtt
     3901 gataacttaa aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga
     3961 aaaaacatga gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatatta
     4021 atagtttttg tggtcttagg gagactaaat agtccttact tggattggaa ttataccgac
     4081 cctgaaaccg ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta
     4141 gtaccgattg tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga
     4201 agtaactctg ctttttataa tacagcaagc gttccttaaa agcgtcattc tacatggcaa
     4261 tcgaggacga ctatgtaatg aagaaccttt ttgatttcca actaagcgaa gttattgagg
     4321 acgacagtag gcccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat
     4381 actctcatgc gtcagatgca ggaaatgcga ggtctggtag catgatttac aacgatttgt
     4441 acaactattg cgagtagaaa tatatggaga taagtattat ctacaaagtc agaaaagaaa
     4501 tgatttgata atttacccat gttataatta ttgatgtgaa atcaataaat caaaattcct
     4561 ttttgggatt taacaataag gaaaaagtga atgacttttg caaggaggtg ttaatatcag
     4621 acaagtaaag tcttttgttt ttccagttat atccttaata ttcttagacc aaattagcaa
     4681 agttcttata ggattattct taatggactt tgaaattgat ataattggga aatttttaag
     4741 attcaatcct gttcaaaata caaaattaag tctctgctat ctaacaaacg gagcacaaaa
     4801 ataaaaatta aatataaagc acagttaatt ttgattaaaa agttgactgt cttttctttt
     4861 gtagaaattt aagcataggt aataaagaaa tgactgcatt taagcattat gtatataatt
     4921 ggaggcaata aaaagccagc aaagcccata aaatagagct gtgctagcaa catgatgaat
     4981 tataagaaga taatcaaaaa cttacgcttt attttaattt aaaaaaatga ttactgtaaa
     5041 gaaatcattt tttattataa tcactgatac atcaaatatt ctaaataaaa tttttattta
     5101 tctggaattg tatatttttt ctataatatt aactacagcg tacattgccc aagcacagat
     5161 agccaataca aatactccca tcattactaa atctaattta aatacttgag aaccatatac
     5221 tatcaagtag cctattccat atcttgaaac taaaaattct ccaactatta cacctaccca
     5281 tgccatacca atattaattt tagttaaatt tattaaattt cctgtgtttg caggtaatat
     5341 gagtttaaat aatatttgag atttagtagc tccaaaactt tttaacatct taattttttc
     5401 ttcatctata ttcataaaat aattataagc tgaaagtata gtaacaacaa cagaaatcgt
     5461 tacagctgtt acgacgattc cttctattcc agcacctacc caaactataa ttataggagc
     5521 taaagcagtc ttaggaagag catttaaaac taccaaaaat gggtctaata tttttgataa
     5581 tttttctgac caccaaagag caattgctac caaaatacct aatacagtac ctataacaag
     5641 accaagaaca gtttcatata cagatattcc aatatgttta aataattgac cactaaatgc
     5701 atattgaata aataaattgt aaatatcact tggtttgcta aatagaaatg tatttataac
     5761 atttaagtca gctaaaagtt cccagattac aataaaacca attaaaatta atagctggta
     5821 gaataataca tttctttttt ctctttttac acctttaaca tagtttatat atccttcaga
     5881 tcttttatta agcctcatgt gtatctagct ccttccataa tatgtcgaaa tacttttgaa
     5941 aatcaggaac tttacgtgtt ttgagaggcg tttttgttcc atccatgtta agttcaatat
     6001 cgtaaatatt ttttatggta gctggtcttt tagatagtat tgcaacctta tctgatatgc
     6061 ttattgcttc tgctatagct aaaaaagtgc aactaattgc ttaaagaaac agaattatca
     6121 acttcagcac ccatataaac aatattaaga tttcctaaac tgctatccca aacgatacta
     6181 ttaataatag tagataataa caatctctta tcatctaaac tggcattatc aacttcatca
     6241 ttaaatctct tcaaattact aacaataata tcaatattaa tactgtcact actaatattg
     6301 atgttactag attccaaaat attaatctta tcctttaaaa tactaatctc attatttagg
     6361 ctctcaatct gggaaactat ataatcagaa gcagtactac cagtattttt tgcaagctct
     6421 ataactaaat ttttaataag tctttcctta tctaaaattt gatttttaag taaattagtc
     6481 tcagatttaa aaatattaaa aacattatca gtcttaatgt tattactatt gaatgcactt
     6541 ataatagact cttctttaca attcttaatt ttatctataa ctaaactttc aactaatgga
     6601 ccattaagat ttttacagtt gcaactatca gacccaagtg tttttttagt accacaaata
     6661 taataatact ttaaatcctt gtcagttttt ttgcctttat aggtaattct catattagaa
     6721 ccacatttac cacattttat aagaccagat aaaagagcag tttttccagt tgatatcctt
     6781 ggagttttat cacgattttt atctaattga ttttgtactt caagccatat ttcaggttct
     6841 atgactccat ggtgttttgc aattgctgct attgagttag atgtattttt agcataggta
     6901 aggattccac attttctatc tggagtacct acaacatcca taccactatt tttaaggtaa
     6961 tctaaaattt catcattagc ttttacatat gctggatttc taagtattct agataaagaa
     7021 ctagggtcaa atttgccacc acgaggaccc cttattccat tattatagac atatttatat
     7081 aacttagata aggacctaag ttctaaatat ttattgtata ttagctttac aatctccatt
     7141 tgttcatcat ttacttgaag cttatacata cttctttctt ttagattttc atctaagaat
     7201 gtgatttttt cactgttgaa tccatgaggt gtattgcctc caagccatct accactacgg
     7261 gctaattcat acatattgtc ttttatacgt tctgctattg tttctctttc taattgagca
     7321 aatacacttg atataaacat catagctctt cccattggag ttgaagtatc aaattgttct
     7381 ttgatagata taaatgatat gtttagttca tttaattttt ctattaaagt agagaagtca
     7441 gatacatttc tcgatatcct atctaatcta taacaaacta ggtaactaaa tttcttatct
     7501 ttggcatcct gcatcatttt cttaaattgt ggtctatcta tattgctacc actaaatccc
     7561 tcatcttcat atatcaaaaa atcttttaca ccaatagact ttgcataatc aatacataat
     7621 tgtatttgat tttctataga atcacctttt ccagtaaatc ttgattttct tgaataaatt
     7681 gcagctttca aaattaaaac ctcctataag ttaggatata tatttgaaac tatttatcta
     7741 caacctttta agtaaactta ttagaatgaa tttattaatt aggtaaattt atataattct
     7801 actttttata atatatgact atttatttta tttctttact attcaagttc taaatcaata
     7861 ataaattttg atatttaatt gtatcaggac aaatctctaa tttcatagat tattaatata
     7921 aaatcatagg ttagtgtgaa aaagttgaaa tttaaatatt ttaaaagata atttttttga
     7981 actaataggt ataacaaaaa tttatagggg gattgcatat ggaaatcaat gttatagaaa
     8041 ttttccctaa agataaagct aaacttaata aaatagaaat ggataaagct agttggtttg
     8101 taaatataat aagtaaaaaa tatcctaaag aagctttaaa tgaagcattt agtactttag
     8161 aaaaagaatt aaatataagt aaagctaata cataagcttt aattattgat tttatatact
     8221 attggtttta tacactatga cttgacatta tattaatcaa atagtatatt tttaaatata
     8281 gcacaatgaa gtgttaaaag agcaatggag ctccatagat aaagtctatg gagctttttt
     8341 gcgtatttaa cttaaggagg ataggacttt gaataacttt gagattaaaa ctagaataaa
     8401 atttggagaa ggctcactaa aagctttgca agaattaaaa aacaaaaatg tactaataat
     8461 aacagaccct tttatggtga aatcaaaaac aatagataaa ataacatcta atttaataaa
     8521 ctcaacatat caagtttttg aagaaatagt accagaccca ccaatagaag tagtagtagc
     8581 aggaatagaa gcatttaaat ctataaatcc agacgtaata atagcattag gtggtggttc
     8641 tgcaatagat gcagcaaaat ctataatgga cttttctaaa aaaatattaa aaattaacga
     8701 agtagagttt atagcaatac caacaacaag tggaacaggt tcagaagtaa catcatttgc
     8761 tgtaataaca gatagtcaaa aaaaatgtaa gtatccatta gtgagtgatg atttacttcc
     8821 agatatggca atactagacc cagagcttgt aaaaactgta ccaaatttta taacagcaga
     8881 tacagggatg gatgtattga cacatgctat agaagcttac gtatctacaa attctaatga
     8941 cttttcagat gcactagctg aaaaagctat aaagttagta tttaaatact taataaaagc
     9001 atataaagat ggaaatgaca tagaagctcg agaaaaaatg cacaatgcat cttgtttagc
     9061 aggtatggca tttaaccaag catcacttgg cataaatcat tctattgcac atgtattagg
     9121 tggaaaattc catataccac atggaagaac aaactctata ttacttcctc atgtaataga
     9181 atataattca tcaatagtag gatatactaa cacaacttac tctgctacag ctaaaaaata
     9241 tgcacaaata gcagaattag taggacttgg gagtgctaat ataagaatat ctgttagaaa
     9301 tttagtaaat gaaataaaga aactacaaaa agaaatgaat atgccaacta aattgacaca
     9361 atgcaaatta agtttagaag atataactaa tgaagaaaga gaaatagcaa aacttgctat
     9421 aaaagatact tgtacattaa ctaatcctaa ggtaccaact gaagatgata taataaacat
     9481 attaaagaac attaaataac tacattttat tacatgtaaa tataatatta tacaaatagg
     9541 gaaaatgttt ttaaaaatat aaattatata ttgacaaata taaaacataa aactataatg
     9601 aaagtatcac aaaggcgtga tactttagta gaaagcaatg gagcttctta ggaatattaa
     9661 tattattctt atggagctcc ttttatttta atcaaatagc tttttaataa actatattca
     9721 aagaaagcag ggaataaatt tgactgaaga atctaagcaa agagttattc aagaatatgt
     9781 tcctggaaaa caggttacat tagcacatat catagctaac cctaatgaag acatatataa
     9841 gaagttagga ttagtacttg ataaaaaaga tgcaatagga atacttacaa taacaccaag
     9901 tgaagcgtca ataatagcag cagatgttgc tacaaaggct tcaaatgtat ctttaggttt
     9961 tatagataga tttagtggtt cagtagtaat atcaggtgat gtaagttctg tagaatcagc
    10021 gttaaatgat gttttagaag tcttaggaaa tatgttaaac ttctcatcta caaaaatcac
    10081 aaggacataa taatacaatg aagaatataa tatttatggg aaagacaggt agtggtaaaa
    10141 ctaccttatg tcaaaaacta gaccagttag aattaaagta taaaaaaact caatcagtgg
    10201 aactttataa taatgcaata gacactcctg gtgagtatat ggaaaataga aacctatata
    10261 atgccttaat aacaacatca gcagatgcta aaattatagc aatagtgtat gatgccacac
    10321 aagaagaaaa ttatatagca ccaggttttg ccagtatgtt ttgcaaagac gtaataggaa
    10381 ttataactaa aataaataaa gctactgaaa acgaagtcaa tatagcttat gaaagactat
    10441 cactagcagg agcaagtcaa atctttaaaa tagacacagt tgataatatt ggacttgaaa
    10501 aattatttaa atatctagaa aaatataaaa atatagatga agtttaaggg gaaatatttt
    10561 tttattcaaa aatattatta aagtatgata aaataaaaaa atgtataaac tattttaaga
    10621 agatttaatc tttaagcgag gtaggaacat ggggaaaaaa atactattgg ttgatgatga
    10681 accactgtta aggatggata caaaagatat attagaaagt catggctatg aaataatagg
    10741 cgaagcaagt gatggatttg aagctgttga aatgtgtaaa aaacacaaac ctgatttagt
    10801 tataatggat atagagatgc caatacttga tggtataaag gcaggcaaaa taatatgtaa
    10861 agaaaatcta gctggtggag tacttttatt aacttcattt gaagataaag aatacgtaga
    10921 aaaagctaaa agtataggtg cttatggata tttagtaaaa acagcaagtg aaaaatcact
    10981 tatcccaacc atagaaatgt gtttaaataa ggtaaatgaa tttgaaaaaa tgaaaaaaga
    11041 tttagaaaaa gtaaattcta aattgaatga aagaaaatta gtagaaagag caaaaggtat
    11101 acttataaga gagtttaaga tttctgagga tgaagcatac actagaataa gaaaattaag
    11161 tatggataaa agaactacaa tgatggaaat tgccaaaatg attataatag gatacgatga
    11221 ctaatacgat aaaaaaaata tcaactgcac aaacatactt aaataataac gaaattgaaa
    11281 aaattataga agtatcaaaa tccttagatt taatggcttc tttttatgaa gctgatgttt
    11341 ttatagatgt actaagtaaa tttgatgatg aagcaattgt tgtggcacat ggtagaccta
    11401 aagaaaagtc aatgtactgt gaaaatgtta taggtaaaaa ggctttaatt gaaaatgaac
    11461 caggagttat aaagtgctta aaaacaggag aaagagtaag agatattaaa gctttaacac
    11521 aggaatataa actggtcaaa caaaatattc agccaataac actagatgac aaagttttag
    11581 cagtactaat agttgaaaaa gatataagta gtgaagttaa gaatgagttt aatgctagtg
    11641 aagacgaaga aaataaaaat ttacatcagt ttataaaact tatcggaaat aatgactttt
    11701 tagttgataa tttaaattca gccatattaa tatttgataa aaatggaaaa ttaaaaatta
    11761 aaaatcgtat tgcaactgag atatacaaaa atcttgggtt tggatatgat atacaagata
    11821 tgcattacaa tgatttagat atagaaacca acacctttga aaatatatta atggatgaaa
    11881 attatgatga gacaaatgaa gtttcaatag gtaatatgtt ttttaaaata aagaccatat
    11941 acatcaaaga tgatgaattt aaggtttgca aaatagtaca agatataagt gatttaaaga
    12001 gaaaagaaga agaattagtt ttgaaaacag tggctataag agaagctcat catagagtaa
    12061 aaaataatct tcatacagta atatctataa taaaaaaaca aagtagatta tctcaaaatg
    12121 atgaagtaaa gcagtgttta gataacataa taaatagggt atttgcaata ctttcgactc
    12181 attatttact atctaaagaa gttgataata atatatctat acgagaaggt atcaatttat
    12241 tagtgtcaaa tattcaaggt ggctatttat ttagtaagga tatagatata cgtataactg
    12301 gtgaagattt taaaattagt ggagaaaaag caacagcaat acttttggtt ataaatgaaa
    12361 tactacaaaa ttgttatgac catgcatttt cggatagaga acatggtact atacaaatat
    12421 taataaataa agataacgac tatagaaata ttgctattat agatgatgga attggatttg
    12481 atagtaataa tataaataaa aatagcttag ggacatatat aatagatagt tatataacac
    12541 agatgttaaa aggaaatata gagagaaaaa gtagtgagaa tggtacagaa gtattaatta
    12601 ctatacctac acaaattaat atttaaataa taactcatct acaaagtagt agttttaatt
    12661 gagcaaagag gcttaaggtt attaatagat taagttaatt gaatgtattt ataaaaatta
    12721 taagtatata ttcaatttaa caggtattta ttataacttt aagcctcttt gcttgtttaa
    12781 gacatactta aattttagga ggtgagaaaa tgagagaaga gttattaagt gttggtattg
    12841 acattggaac aagtactact cagttaattt tttctaaatt aatagtagaa aatactgaat
    12901 caaatttttc ggtacctaga ataagtatag tagacaaaga aattatttat aaaagcaaag
    12961 tttatttcac ccctcttatt tcaaataatg aaataaatgg tcaacaaata aaagatatag
    13021 ttgaaaatga atataaaaaa gcaaatatat ctaaagaaga tatacataca ggagcagtaa
    13081 taataactgg tgaaacagct agaaaagaaa atgcaaatga tgtacttcat actttgagtg
    13141 gatttgctgg ggacttcgtt gttgcaacag caggacctga tttagaaagt ataatatcag
    13201 gaaaaggagc tggagctcat atttattcaa aagaacattc aactacaatt gtaaatatag
    13261 acataggtgg gggaactagt aacatagcct catttaaaag gggagaagta accgatactg
    13321 ggtgtttgga tataggtgga aggcttgtaa agatagataa aataagtaaa aaaataactt
    13381 atatatcacc taaaatagat aaaatcataa aagataataa tttaaatctt caagtaggca
    13441 caatggctac tgtagaaaat acaaagccag tagtggatat aatggttgac ttattaatgg
    13501 aaagcatagg actaaagaaa cagacacctt tgtttgattt tataataacc aataaatcaa
    13561 taagtataga tgaaactatt aaaaatgttt cattctctgg tggagtagct gattatattt
    13621 actatgatgg agaaataaaa gattattttg aatatggtga cttgggaatt ttacttgggc
    13681 aagcaataaa aaattgtgaa taccttaaaa aagtaaatgt agttaaatct atggaaacaa
    13741 taagagccac agtggttgga gcaggttctc atactactga agtaagtgga agtacaataa
    13801 cttacactag cgaaaagttt ccacttaaga acttgccagt tttaaaatta tcaattgatg
    13861 aagaaacagg cacttcaaaa caaatcagtg aagctataaa aaataaactt aattggttta
    13921 aactagaaaa tgatttacag aaagttgcta tagccatgat aggaaagaaa aatccaacct
    13981 ttttagatat acaagagtat gctaaagggt ttgtagatgg aatgcaacaa atcatagaaa
    14041 atgatttacc ttttatagta attgttgaaa atgatatggc aaaagtgtta ggtcaagcaa
    14101 tatatgcatt attaaattat gaaaaagaaa ttgtttgtat agacagtgtc aaggttgaaa
    14161 atggagacta tatcgacatt ggaaaaccta tagttgaagg taaagtttta cctgtggtaa
    14221 taaaaacatt agtttttaat taatagaagg aggtaagtaa atgattttaa atacaaaatt
    14281 atttggacgt acttatcagt ttaaaggctt atatgatgta atggctaaag ctaatgaaga
    14341 aaaatcagga gataaattag caggtcttgc agcagaatca gctgaagaaa gaatagcagc
    14401 aaaagttgtt ttatcacaca taaaattgga ggatataaga aataatcctg cagtggcata
    14461 tgaagaagat gaagtaacaa gaataataca agatgctgta aatgaaaaag tttatgaaga
    14521 aataaaaaat tggacagtct ctgaacttag agaatggcta ttggattcaa atactacaag
    14581 tcttgatata agaagaatat caagaggatt aacttctgaa atgatagcag cagttgcaaa
    14641 attaatgtca aatatggact tagtttatgc agctaaaaag ataaaggtta ctgctcactg
    14701 taatacaact ataggagaac aaggaacatt atcagcaaga cttcaaccta atcatccaac
    14761 agacgaccca gatggaatac ttgcatcact tcttgaaggt ttaacttttg gtataggaga
    14821 tgcagtactt ggattaaatc cagttgatga ctctgttgag agtgttacta aagtattaaa
    14881 aagatttgaa gaagtaaaac aaaaatatca aataccaact caaacatgtg tattggcaca
    14941 cgttactaca caaatggaag caataagaaa tggtgctcct gcagatttaa tattccaatc
    15001 tatagcaggt tctgaaaaag gaaatgaagc atttggattt aacggcgaca ctatagaaga
    15061 agctagacag ttagccttaa aacacggtac agctacagga cctaacgtta tgtattttga
    15121 aactggacaa ggttcagaat tatcttcaga agcacatcat ggaatagacc aagttactat
    15181 ggaagctaga tgctatggat ttgctaaaaa gtatgaacca ttcttagtaa atacagttgt
    15241 tgggtttata ggacctgagt acttatatga tgctagagaa gttgtaagag caggacttga
    15301 agaccacttt atgggtaaac tacatggctt accaatgggt gttgatgttt gttacactaa
    15361 ccatatgaag gctgaccaaa atgatatgga aaacttagcc atactattaa caacagcagg
    15421 atgtacgtat tttatgggaa tacctcatgg agatgatgtt atgcttaact atcaaacaac
    15481 aggataccat gaaacagcat cacttagaga aatgtttgga ctaacagcaa taaaagaatt
    15541 tgatgcttgg atggaaaaga tgggattctc taaagatggt aagttaacag agttggcggg
    15601 agacgcatct gtattattag gataggaggt atttaatatg aatgagaagg atttaaaagc
    15661 attggtagaa caattagttg gacaaatggt tggagaatta gatactaatg ttgtgtctga
    15721 gactgttaaa aaggcaactg aagttgtagt agacaacaat gcttgtatag atgatataac
    15781 agaagttgat ataagaaagc aattactagt aaaaaatcct aaggatgcag aagcttattt
    15841 agatatgaaa gcaaaaacac ctgcaagatt aggtatagga agagctggta ctagatataa
    15901 aacagaaaca gttttaagat ttagagcaga ccatgctgct gcacaagatg ctgtattctc
    15961 ttatgtagat gaagaattta taaaagaaaa taatatgttt gcagtggaaa ctttatgtaa
    16021 agataaagat gaatacttaa caagaccaga tttgggaaga aaattttctc cagaaactat
    16081 aaataatata aaatctaaat ttggtacaaa tcaaaaggtt ttaatattag ttggagatgg
    16141 gcttagctca gctgcaatag aggcaaattt aaaagactgt gtgccagcta taaaacaggg
    16201 tctaaaaatg tatggtatag attcaagtga aattttattt gttaaacatt gtagagttgg
    16261 tgctatggac cacttaggtg aagaattagg ttgtgaagtt atatgtatgt tggttggaga
    16321 aagaccagga ttagttactg ctgaatcaat gtcagcatat atagcttata agccatatat
    16381 aggaatggca gaagcaaaaa gaacagttat atcaaacatt cacaaaggtg gaacaactgc
    16441 agttgaagca ggtgctcata tagctgaact tataaaaact atgttagata aaaaagcttc
    16501 aggaatagac cttaaataaa gtcgagggag tgtagaatat gaaaaatgat gtaattcgtc
    16561 ctaatatact aggaattaaa ataatttcaa atataagtcc agaaatggca caaaaactag
    16621 aattaaaatc acatcacaaa agcttaggct taataactgc agactgtgat gatgttacgt
    16681 atacagctct tgacgaagca actaaagctt cagaagttga tgttgtatat gcaaaatcaa
    16741 tgtatgcagg tgctgcaaat gcttcaacaa aacttgctgg tgaagtatta ggaataatag
    16801 caggtccaag cccagcagaa gtaagaagtg gtctaaatgc agtagttgat tttctagagt
    16861 atggagcaac ttttataagt gcaaatgatg atgattcaat agcatactat gctcactgtg
    16921 tatcaagaac aggtacatac ctttcagagg tagcaggaat aagagaaggt gaagcattag
    16981 cttatttagt agcaccacca cttgaagcaa tgtatgcact agatgcagca atgaaagctg
    17041 cagatgttaa gatgtgtgaa ttatttgcac ctccaactga aacaaacttt ggtggagcat
    17101 tacttacagg ttctcaatcg gcttgtaaag cagcatgtga tgcatttgca gaagcagtaa
    17161 aatcagttgc agacaatcca acaggatttt aaggatagtt agatggatgc tataggctta
    17221 atagaagtaa taggatatgt tgctgcaata gaagcttctg atgcttgttt aaaatctgca
    17281 aatgttaata ttgtaagagt tgataaggtt ggagcaggta ttgttacttt aacaatatgt
    17341 ggagatgtcg gtgctgtaaa agctgcttta gaagcaggtg aaatagcagc ttccagagtt
    17401 ggtacattaa gaacatcaca tattataccg agaatccata atgaagtaac aaatgcttta
    17461 ttcaaaacta aagaacctaa agtctgtgaa gtttcagaag ataaagaaaa aaactttgag
    17521 attacattgg aaatagctga taatgtatcg aaaaatttat cagaaaaaga aaatgtaatt
    17581 ttagatgtca atgaaaacag tggagatact gaaaatttat atagtgatat agataaaaca
    17641 aacaaaaaaa ttgaaacagt tgatgatagt ttaacagaag ctaatgagga aaaaatagaa
    17701 aacactaatg ataaagaagt attggaacaa gttaaaatag aaaattccga ttttgataga
    17761 gatgacctat caaagaacag tgtaaaaaaa cttaaagcta tggctaagaa attagattct
    17821 tcaataactt ataaagattt aaatgttctt aaaaaagaag aattaataaa cttagttaaa
    17881 aagcttagta gaagggatag atagttatga cacttttaga taaagattta gtatctatac
    17941 aagaagtaag aactcttgta agaagatcta aagtggctca agagaaactt tctaaaatga
    18001 gtcaagagca aatagataaa atagtaaaag ctgttgtaga tgcagcattt aataatgcta
    18061 ctaaattggc taaaatggca aatgaagaaa ctggatttgg aaaatgggaa gataaagtaa
    18121 ttaaaaatac ttttgcatct atgggagttt ataaaacttt aaagaacaca aaaactattg
    18181 gaataattgg tgaagataaa gaagccaata cgatagatat agctacacca gttggagttg
    18241 ttgcaggtct tataccttca acaaacccaa catcaacagt aatatacaaa actataatat
    18301 ctctaaaagc aggaaatact atagttttct ctccacatcc aaatgcaaaa aactgcatac
    18361 ttgaaacagt taatataata agtgaggctg ctataaaggc tggagctcca gaggattcaa
    18421 tattatgtat aactaatcca agtatagttg gaaccaatga attgatgaaa cataaagata
    18481 caaacttaat actagcaact ggaggagaag caatggttag agctgcttac tcttcaggaa
    18541 caccagctat tggagttgga ccaggaaatg gaccagcatt tatagataaa tcagcaaatg
    18601 ttaaattagc aatcaaaaga ataatagatt ctaaaacttt tgataatggt gttatatgtg
    18661 catcagaaca atctatagta atagaaaaat ctatggaaga aactgtttta aaagagctta
    18721 aaaatcaagg tgcatatgtt ttaaatgaag aggaaagtga aagattagca aaattcatat
    18781 taagagctaa tggtactatg aatccacaaa tagttggtaa aagtgttgag gtaatagcaa
    18841 atttagcaga cttaacaaat gtacctaaaa atacaaaagt tctagttgca agagaaaata
    18901 aagttggtca caatgtagcc tattcaagag aaaaacttac accaatacta gcgttctatg
    18961 ttgaagaaaa tgtagaagct attatgaaca gatgtagaga aatattgtta aatgaaggtg
    19021 caggtcatac attctcaatg catgtaaatg atgaagaatt ggtaaaaaga tttgccctta
    19081 atatgccagt atcaagaata cttgtgaata ctccttcagc acttggagga ataggtgcat
    19141 caacaaacct tgtaccagca ctaacacttg gttgtggtgc aataggtgga agttcaactt
    19201 caaacaatat aggaccactt gatttaataa atgttaaaag agtagcttat ggaatcaaag
    19261 aaatagaaga acttggaaga gataaatctc aagttgaaaa atcaatgttt gactcaatgg
    19321 ataaagaaga attaataaat cttcttgttc aaaaactagt caaagaacta gcataactaa
    19381 aataaacaat gaaacttatg gctatcgcta taaatttcta aaaatataaa ctataaaaaa
    19441 ctataaaatt aaatttttgg aggataagat tatggcaagt gcaaacgcat taggaatgat
    19501 agaaactaaa ggattagtag gagcaataga agcagcagat gcaatggtta aagctgcaaa
    19561 tgtacaatta gtaggaaaag agcaagtagg tggaggatta gttactgtaa tggtaagagg
    19621 agatgttgga gctgttaaag ctgctacaga tgcaggagct gccgctgccg agagagttgg
    19681 agaactaata tcagttcatg ttataccaag accacacttt gaagtagatg ctatattacc
    19741 aaaggtatca gctgagtaat ataagaataa ttataaaatt taaattttga ttgaaaaaga
    19801 ggctatctca aaaatgagtc ttactaataa actcaatgta ttttgaggta gtctttttta
    19861 tacatctaat aggagatgtg ataaaatggg tgttttaaca gaaaacgaag taaaaagaaa
    19921 aattggaaaa aacaaaaata atataactga attttatata gaaaaaggtc aaataataac
    19981 tccttctgca aaatcttatt tagctgagag aagtattgag cttaaatatg ttgataaact
    20041 ttcagatgta aacaatgaac ctaaagaagt tgtaaaagaa gtagttaaag agaaaaaata
    20101 taaatatact actgtatttg gcgccaaatt ggatgaaaaa ccagaacata tgactcaatt
    20161 aaatggaaat cttttagttt ttaaagacca taaaagaata atattgagag gaaaaataga
    20221 ttctcttgaa tcagaaatac taaaggtaca gatactttgt caaaaactag atatgccaaa
    20281 gcttgttaag gaattgcaag aagtattaga ctttgtaaga aatataatga gatgtgaagt
    20341 tttagatgaa aagctagggg actttaaact tttaggatta actcctcaag aacttagaga
    20401 acaatctcac tatcctaaaa aatactttgg tataggacat gagttcccaa gttatgaaat
    20461 gggagaagta gtagtagcta taaatagtat aagaagtaat acacgagagg ttgaacttgc
    20521 agcctatgaa gcatttaagg gtgagtatgg aaatgttcac agagaagatt taataaaggc
    20581 acttaataga ttatcgtcag tattttggat aatgatatat aaggttagaa ctaataagta
    20641 taagtagttg gaggacataa tggagaatct attaaatgaa atagtggaag aagtaataac
    20701 tagagttaaa aaagaagcat ttatagaagt tgaagcatca ggaagacacg ttcacttaag
    20761 tagagaagat gtcgataaat tatttggaga aggatatact cttacaaaac taaaagacct
    20821 atctcaacca ggtcaatatg catgtaagga aagagttaca ataactggtc ctaaaggaag
    20881 tataaaaaat gtagttgtac ttggaccatg tagaaatgaa actcaggtag aaatatcttt
    20941 aacagatggt tcaactcttg gactaaaagc tcctataaaa caaagtggag acttagaagg
    21001 tactttaagt attaaaatat caacacaata tggtgaagtt gaacttgata aggggctaat
    21061 ggttgctaaa agacatatac acatgactcc taaagatgct gaaaaattca atgtatgtga
    21121 taaagagata gttgaagcta aggtgatggg tcaaagacct ttaacatttg atgatgtagt
    21181 aataagagtg agtgacagtt ttaaaactta tatgcatata gactatgatg aagccaatgc
    21241 atgtggttat tcaaaaggaa ctgttgctaa aataataaag aaagcataaa tttaacctac
    21301 tatataaatt acctctaggg aggatttaat atgaattatg ataaccttgt agatttaata
    21361 acggaagaaa tctataaaaa aataaatagt aatgaaataa aaatatctaa taaacctaaa
    21421 gctgtgattg tatttgaaca agataacaat aaatttaact tattaaaaga tgaatttgaa
    21481 atagtagaat ttgataaaag tataagggaa tgtgaaatag tgatagtttc aagactttgt
    21541 atgagggggc tatcgaatat agcacttgga aattcaacaa gtgatgaaga aagatttata
    21601 ctaaaaatga ttatgaaggg caaaaaagta tatgtcctag atgaaggaat tgaatacaaa
    21661 aaatataagg atactgcacc aaaagcatta tataagaaat ttatgtcatt tgaagatgaa
    21721 atatgtaaat ttggtgtaga aattataaaa gacttaaatg ttataactaa aaataagtta
    21781 aatataaaga aagaactaga aagtactaga ggttctaaaa aagaagatac aaaagtttta
    21841 gataaagatg ttttaaattt aaccagtgtc gaaagtgtaa aaatagataa tgaatttagt
    21901 ttagatttaa ggaataaaaa acttatatcg gaagctgact taagaaaacc aactataaat
    21961 ggagttaaaa acatattagt taataaaaaa agtataataa caccattagc tgtagatttt
    22021 atgagaatac accatctcaa actaaagaaa ctgtaaggag gtaattttat ggatatagga
    22081 aaagttgtag gtaatgtttg ggctacaaga aaagatgaaa aattatgtgg ccaaaaactt
    22141 ttagttgtaa aaattttaaa aactaaagac acatacaagg aaggcttcat ggttgcagcc
    22201 gataatgcag gagcaggaaa tggagactta gttcttatat ccaaaggttc ctcagctaga
    22261 gagtcgattg aaaaatctca tgttccagta gatgcaacta tagttggagt agtcgattcg
    22321 tttgaggtgt ttgatgaata aaagtatagg agctatagag tttaaaagca ttgctaaagg
    22381 tatagaagta tccaatgaaa tgataaagaa atcttcagtt gatgtattgt acttaaaaag
    22441 tatttgccca ggtaaatttc taataatagt aggtggagaa acttcttata taaatgaatg
    22501 tgtagattat ggaataaaac ttggagaagg atatattgta gataacttcg taataaatgc
    22561 aatttcacaa gaaatattag atggatttaa aaataagtat caaaagctag atagtatagt
    22621 ctctataggc gttgttgaaa gtagtaaagt atgcgctgga ataaaaatgc ttgataagac
    22681 tttaaaatct ggcgacttag tattagtaaa acttcaatta tcttttgcta taggtggcaa
    22741 attagtatat atagtagcag gagatttaag tagtttagaa tatgcattaa aagaaagtga
    22801 aaatgtagta agagaaaaag aagtaatata taagactgta ataccatcag tagacagtca
    22861 aataataaaa agtctaataa aataatgggg ggaattatga gtataaacga aattataatc
    22921 tatgtaatgg tagtcttcat ggtacttgga gctatagata aatgtatagg taacaaattt
    22981 ggtcttggag aacaatttga agaaggtata atggcaatgg ggtcacttgc agttgcaatg
    23041 gttggtgtta tatgtttagc accagttctt gctgatgtat taagaccaat tgtagtacca
    23101 gtatttaata tacttggagc tgacccagct atgtttgctg gaagtttatt agctaatgat
    23161 atgggtggtg cgccacttgc acttgaactt gcgaaagacc ctaatgctgg gttatttggt
    23221 ggattaatag taggggctat gatgggacct acaatagtat ttataatacc agttgcgctt
    23281 ggaataattg aaaaagaaga ccaaaaattc ttagcaacag gaatacttgc aggtataata
    23341 acaataccta ttggagcatt cgttggtgga ttagttgcag gattcgaggt aatgatggtt
    23401 cttagaaact tagtgccaat aataatattt gcagtagtta tagcacttgg attattaaaa
    23461 ttcgaaaatg ctatgataaa aggattcaca tactttggaa aaggtgtagt aatagttata
    23521 actttaggac ttgctgctgc catagttgaa gctttaacag gaatagttgt aatacctgga
    23581 atgaaaccaa tcagtgaagg tatagaaata gttggagata tagcaatagt tttagcagga
    23641 gcattcccat tagtgtttgt tataacaaag gtatttaata aaccattaat ggctttagga
    23701 aaagttcttg gaatgaatga tgtatcagca gcaggccttg ttgcaagttt agctaactgt
    23761 ataccaatgt ttggtatgat gaaagatatg gacaatagag gaaaaataat aaatgtagca
    23821 ttctctgtat cagcagcatt cgtatttgga gaccacttag gatttacggc aggatttaat
    23881 tctaatatga taacaccaat gatagttgct aaattagttg gtggcataac agcagtattg
    23941 ttagctatgg ttatcgcaaa taagacacta aagaaggaga atgtataaga tggatatatc
    24001 aaatatagat aaaaatttaa ttgaaacatt agtaagacaa ataatagaag aaaaaataag
    24061 tggaactaaa gacacagtag attttgttag aaataaagac attagtggaa taacttctat
    24121 aaagttacca actgtaaaag ttagtgaatc tgatagatta gatacaggga atccaagtga
    24181 tgtagtttac acaaaagatt tatttacatt agaggaaagc ccaagattag gttgtggtat
    24241 gatggaaatg aaagaaacaa cttttgattg gactctaaac tatgatgaaa tagattatgt
    24301 aatagatgga actttagata taataataga tggaagaaag gtttctgctt cttcaggtga
    24361 attaatattc ataccaaaag gaagtaagat acaattttct gtacctgatt atgctagatt
    24421 tatatatgta acatatccgg cagattgggc atctcaaaat taataaaaat caagttaagt
    24481 taataataat caaataaata atttaaaata caaactctta aataagcaca catatttaaa
    24541 aaagctatga attaaaattc atagcttttt ttataaagaa tgttatattt tagacgtatt
    24601 ttttctatat ttactgggag acatattata ttttttatta aataatctaa taaagtgctc
    24661 tacatttgga taaccaatac tattttactg ataatctttt ccttatcact aatcttacat
    24721 tttaaaatat tatttttact cgtaatatta tcataagaat ttgtttctta tttaaaacta
    24781 attagaatta taaagtttat tgccatgtta ttaataaaat aaatccagtt aaatcgagtg
    24841 ctagttaaag taataagaaa cttttacaat ggtttccttt atcgattgaa ataaaagtta
    24901 atatggtata atatctaaag agagaatctt atcaattaga atatgcatag aaacaagtgg
    24961 cttaaaagga gacgttatat ggaagagata gtaaagaagg aacaagcaag tatgaatgat
    25021 atttttttgt atcttaaagc taaatcaaac tcattatata aatttgtaat attatataat
    25081 gagtttatca ataaacctaa agattatgga actggggaac ttataaacat ggttgaagta
    25141 catatgctaa cagcaattga agaaaatcca ggaataagtc caactcagtt agctaaaata
    25201 tggaacagaa ctaaaggtgc aatatctcaa actatatcta aattggagaa aaagggttat
    25261 ataactaaag aaaagaaaga aggaaatgca aaaactatat tactatatcc aaccacatca
    25321 ggagtagagt taagtaaggc acataaacta tatgacacca tagatgttac aagaacattt
    25381 gaatacttaa tcaaggagtg tacattagag gagatagata gtttttataa agtaattgat
    25441 ttgtttatta atttattaga agaatctccg caggaaaata atgattctga aaaagtgtag
    25501 ctgagattga agttgaataa agagaaatag atattataga agtagtactt aagtacacat
    25561 tatataaaaa tataataggt ataggattaa aaagtttagt ggtttaaagt ttaaccacta
    25621 aactttttta atgtctataa tctactatta tttaatttac taaaaataaa aaaactcatt
    25681 atattaagat aaaaatagaa aaataactag aaaaatacta taatatatca gtttaataca
    25741 gaaaaaagtt aaaaaatttg agaaaaataa aaatgaaaac gttttatatt gacatagaga
    25801 aaaagtagtg ataccattta aatatagttt agtagctaaa ctatatttaa attataataa
    25861 ctattttagg gggatgttat gaatttaaaa caattaagtg aaaaatacga agaatatgta
    25921 attcaaatga gaagagaatt tcataagtat ccagaattat cttttaaaga aataagaaca
    25981 acccaaagaa taagggaaga attacaaaaa ataggggtcc cttttgtatc tattgaacca
    26041 aatatcgtaa tagctactat acaaggcaaa aatgaaggta agacaattgc aattagagga
    26101 gatatagatg cgcttccaat gcaagaagat tcagatgttg aatacaaatc agaatatgat
    26161 ggaataatgc attcttgtgg acatgatgct catggagcta tgttactagg acttgcacat
    26221 atgctaaatg atataagaaa agatttaaaa ggtagagtat tattagtttt tcaagcagct
    26281 gaagaagttg gtggtggaca caaggagata atacagtact ttaaggaaaa tggaggacca
    26341 gatagattaa tagctactca tgtttggtcc gatattgaag ctggtaaaat ttctgttgaa
    26401 ccaggacctc gtatggctgg tgggtctggt tggagaatag acataacagg tagaggaggt
    26461 catggttcta gaccagatca aagcatagat cctataaagc cagcttgtga tatagtttta
    26521 aaagtatctt ctataccagt taatcatata agtgtactag aacaatgtgt tgtacatgca
    26581 tgtgcaatac atggaggaac tacaatagga aatgttattc caaacaatgc agaggtaata
    26641 ggtggatata gatattttac tgaagaatca ggcaaaaaag tgtttgacat tataaagcaa
    26701 atgagtaatg gtgttggaca agtttatgga gtggatgtga aggttactca tactggaggc
    26761 ataggacctg ttatcaatga tgaagaagca gttaagatgg ctaaagaaat agtttctaat
    26821 atagatggat tggagttaga ttcttatgag ccaatatgtg catcagattg ttatggtgaa
    26881 atattaaaag agtattctgg cttttattgt tatctagggg taggtaacaa agaaaaagga
    26941 ataatatatg cacaacacca tcctaagtac aatatagatg aagatacatt aaagttaggt
    27001 tgcgaattta tggctaaata tgcagttgaa tttttaaata aaaataataa ctaaaataaa
    27061 tcgaatttta taaatggggg gatgaaaatg caaggtgcat taaaggaaaa taagaaaaaa
    27121 agtggtggag gatttttagg atggattgaa aaagttggga ataaaatgcc acatcctcta
    27181 gcattattta catatattac gttgatagta gtagcattat ctgcaatagc aagcttttta
    27241 ggtttttcag caacaagtcc tgctacagga aagttaataa aagttatgaa cttaataagt
    27301 gcaaaaggtt tagtactatt tatgcaagag tttgttaaga acttccaaaa cttccctgtg
    27361 cttggagtag ttttagttat ggcagttgcc acaggagttt gtgaaaagac aggatttttt
    27421 tcaactgcca taaaaatgag tttatcaaaa gttaagggga atgcggttgt atttataata
    27481 gcttttatag gagtatttgc taatcaagct ggtgatgctg cttttgtatt agtaccaact
    27541 cttgcaggag caatatttta tggattaaat agaaatccat tagctggtat attctgtgga
    27601 tttgcatcag ttggtggtgg atttgcaaca gctgtaattc ctgggggatg ggacgttacg
    27661 ctaacgccaa taactgtatc agctgctcaa actattcaac ctgcatttga tatgacatta
    27721 ttagctagtt attatgcact atttgtatct gcgtttatag tagctacagt atcaacaata
    27781 gtaacggtta aatttataga acctaaatta ggtaagtata ctggtaaacc agaaggtata
    27841 gatgataatc aaggttttga ggtcacaaaa gaacaagcaa aagcagcaaa gttagcaggt
    27901 tttacagttt tagcttatgt agcgttatta attgctttgt gtataccagc taatagtttt
    27961 ttaagaagtc cagaaggttc tttaatattt ggagcaccac ttatgtcttg cttacagttt
    28021 tttatagtaa tactattctt cttgccaggg attgtgtatg gaatgagggt tggtaaaatc
    28081 aagactgtaa aagacttaaa tgatatatta tgtcaatcaa tggcagcaat ggcaccgttt
    28141 atcgtacttg caatagttat aggtcaattc ttaacgctat tctcagtgtc taatttagca
    28201 caggtactag ctataaaagg tggagcatta ctcaactcat tgccactacc accacaagct
    28261 ataatactgc tattcttagt actagtagcg tttataaata tatttgttat aagcggaagt
    28321 acaaaatgga tgatatttgg accagtattt gttccaatgt taatgcaatt aaacatacat
    28381 ccagcattta ctcagtttgt atatagatta ggagattctg taaccaatca tttatcacca
    28441 ttaaatgcat tctttataat acttttagca acagtccaaa aatacgataa aaatgcaggt
    28501 atgggaacta tattctcagc tatgatacct tacactatag gttactttgt agtacttgca
    28561 gttttagtag tagtttggat gacaataggt ataccagtag gaataggtgg acaagtttgg
    28621 ttataaaaca tatttaataa ttttgggagg aaagaaaatg gaaacaactg aaataagata
    28681 caattttgat gaagtaatag atagaagtgg aacaaactgt cttaaatatg atcttaagag
    28741 tcaatttatg ccagaatctc cagaagatgc actaccacta tgggtagcag atatggattt
    28801 tgcatgtgcc cctgagataa ttaatgcaat gcatgaaaga atagacagaa aaatatttgg
    28861 atatagctct caacaaactg atgattatta tgaagctgta tgtggatggt ttaagagaag
    28921 atttgattgg aaaatagaaa aagagaatat attctttagc ccaggggttg tgcctgcgtt
    28981 aggatttttc acacaaatat taacacaaaa gggtgatggt atcataatag aaaagcctgt
    29041 atactatcct tttgaagata aaataactaa caatgaaaga aaagtagtta atagtccttt
    29101 aaagtttgaa gatagtcatt attctatgga ttatgatgga tttgaagaat tagctaaaga
    29161 tgaaaataat aaggtgttta tattatgtag tcctcataat ccagttggta gagtatggaa
    29221 aaaagaagag cttagaaaaa tagtagacat atgtaagaaa tatgatttgt ggataatatc
    29281 tgatgaaata cattgtgata ttattcgtaa aggtgaaaag catataccat tagaagtagc
    29341 atgtcctgat tataaagata gaataataac ttgtacagct ccaagtaaga gttttaactt
    29401 agctggtatg caattatcaa atatagtaat caataataag gaattacaag aaaaatggat
    29461 gcttgaaaca gattctaaat tagctatatt cctacctaat ccattcgcaa tagttgctac
    29521 taaagttgct tataatgatt gtgaagattg gatgaatcaa gtaaatgagt atatagatgg
    29581 taatattaaa tatgtagaag attttgttaa tgagtatatg cctaaagtaa agataataaa
    29641 atctcaagga acttacttag tttggttaga ctttagaggg tatggattag atgcaaaaca
    29701 gcttgaatat gtaatgctta acaaggcgaa ggtattgcta gatgaaggat acatattcgg
    29761 agaagaagga gaaggatttg aaagaataaa tgttgcttca cctagaacaa ttatagaaga
    29821 gtgtatgaat agaataaagt gtgcatttga aggattataa tagtaatatc taaataatat
    29881 atttgtgtat tttaaaaaaa gactatgcta aaaaaatatt tcaaagcata gtcttttaaa
    29941 gttttatgat gagtttaaaa aatttcaaaa cgttactatt cacttacatg tttatattag
    30001 aaataattcc acaatatact cattttctaa agttgcactt ttatacacct tgcttcacca
    30061 atatcatgtg taaccaaaat agtagactta ttctcattct taataatact ataaacatca
    30121 tcacaaacta ataatctagt ctgataatca agagctgaaa aaggttcatc taaaagtaga
    30181 atatctggat ttacagaaag tgttctaata agcgcaactc tttgtctcat acctccagat
    30241 aattcttttg gatacatact tctaaaatcc cataatccat aagtctttaa aaggctttca
    30301 actctatcta aagatttttt atcttttttg ccttgaattt ccaatccaat agttatatta
    30361 tccattatat ttctccattc aagcaaatta tctttttgaa acatatatcc aatatttcca
    30421 gtaatcttta tatcaccact agtcggtttt aaaagattag taagaatatt taatattgta
    30481 gatttaccac ttccagaagg accaagcaaa gttaatattt ctccttcttt caagttaaaa
    30541 ttgacatcct ttaatacttc aagctcgcct tccttagtgt agaagttcat atttatattt
    30601 ttaacttcta ctatgttgtc attcattatg ccacccccta taacttttag ctcgtatata
    30661 ttatgttatt aacaatatga cctatttgta ccatttttca taaatataat ataaatattt
    30721 tgtttattta cgtattttct tagtaacttg tacgtataaa ttatagataa ataaattata
    30781 aggtagggga taaatttgag taacataata acgaatttaa tagagtcaat atttactaat
    30841 ccaataacta tattgttttt acttgcaata actgtgtatt ctgtttttaa aataatagaa
    30901 aataacagag tatattcatt tgtaagttca aaattagacg aaatcaatag agactataag
    30961 acaagtgagt tttataaaaa ggtaagggat gactactata catatagtaa agaaaatcct
    31021 tatgcagacg taaatataac ttcatttata gaggaagttg tatctgattt aaagcataat
    31081 aatttgccat tgttagaaaa aattagaagt ataaaaaatt catcttcaat aagtatatta
    31141 cttggggttt taggtacgtt tgttggtctt tctacaatgc ttttatgtgt tgatactaaa
    31201 gacattataa attcacttcc ttcaactatt agtagtatgc aaactgcatt tacaactagt
    31261 atatttggag ttgtattttc tctaattata ggttacttta ctaagataaa agattgtgaa
    31321 catgttttga tacaaataat gttaaaatct gaaaatttac taacatctga aataactcat
    31381 tttaagtcag aaagaatgga tttaaaagta gaagaggtaa aaaatacaat aaaacaaata
    31441 agtaagtcta tagaagctat tgaaaaattt gataaaatat ctaaagattt aaatgacttt
    31501 aatgatgaat ttatcagtgg tatagaagct ttaaaaagcc ttttagaagg ttctcaaagt
    31561 tctattaaga catttgacca gagcgtaaga aaattagata aacagtttaa tattttaaat
    31621 ataaaatttg taaagctttt tgataaatat gataatcaag ataatataaa taaagagatt
    31681 ttatttgata taaaagaatc ttcaaaaaat atatacaatg ccactgaaag ccaattcaaa
    31741 attagagatt atataaaaaa tataaatgca ggatttgctc tatatgaaag aagtgctcaa
    31801 gacttattaa ctaaattaat gacccatgaa aataaaatct ctcaaaatca aaaaatcctt
    31861 ttagatgaaa agtttacctt agatgactca ataaaaaatc tctcaagtat tattgaaaac
    31921 ttttctaatg acctacaagt taaactggat atgatgtttg aaaattcttt agatattcaa
    31981 gataagttag atgttatgtt taataactct tttatgaatg atgaagttcc tcttgatagt
    32041 gaaaaattat ttaaggatga tgttaataat gtatttaatc cttttagtga agaaatttat
    32101 gagatagagg ataaagaaat aaaggtcata ggtgaggatg agttaaatga ataataaata
    32161 tataagaact gttaacaggg aatttgaaaa gagtagcttt tggccaacat ttacagattt
    32221 attatctact gtattgatgg ttgtaattct tatcttattt agctcagaaa gtatttcagg
    32281 ttctgttgaa caagatttag ctaagaatgt caatgcttct gttgaagaaa cttttaaaaa
    32341 aagtggaata cctgtcaagg tagataaatc taatgggcaa gtaacttttg gtgaaaaaac
    32401 catgtttgat atagatagtg atgttttaaa accagaggct aaagaaatgc taaaaatgtt
    32461 tgttccaaag tacatagaga ctatttataa agattatggt gactatatat ccaaaatagt
    32521 tatagaaggt catacagatg atgtgggaag ttatatatac aacttagatt tatcgcaaag
    32581 aagagcatat agcgttgcta aatttatagt gggagatgaa ataggtgact ataaatacaa
    32641 agataaagtg actaagcata ttattgcaat aggaaggtca aaagctgaac tcatcaaaaa
    32701 tggtgacaat agcgtgaata gagatgcatc tagaagagta gaactaaaat acgaaataaa
    32761 tattaatcaa aataaataat taaattttta taaaaaaaaa gaaggcagtt ggtgtgagct
    32821 ttaagccttc tttaaggaaa aagtttattt atttttgttg atacttaaca tattatagtt
    32881 tttagaagca gtttgcaata tattaaacgt aaatggtcgt ttaaaatgca taactggata
    32941 ctactgatgt aaaatatttc attttatata atataaaaat tatacagtgt caattttcta
    33001 tattattgta ctataatcta ttatttaaaa tattatgtaa aaaaatatca taaaaacgca
    33061 tatcatgtta taattactag agtattatga atatggaggc gtagtttgtg atagaaataa
    33121 gtataaaaaa taaaaaaaat tctaaaatta aaactttgat acttttattg atagtatcta
    33181 tatcgattta tttaactggt tgtactacat cagataatgg aaataatcaa tcaaataata
    33241 atgattcttt gtctagtagt atatcaagtg ataagaatgt aaaaattcat tttattgaca
    33301 ctggaaatag tgatgcaata ttgattcaag atggtaaaac ttttacattg atagatgggg
    33361 gagataatga tgatgaggga ttaatggtag attacctaaa caatcaaggt gtaaaagata
    33421 ttaaatatct tatagctacc cattctcatg cagaccattt aggtggactt gattctgttg
    33481 tcaaaaactt taatatagaa aatgtttttg tatcgaatgg ttcagcagaa acaaagagtt
    33541 atagggactt tataaatgct ttagcaaata agggtttatc cccaagtgta ccgcttgaaa
    33601 ataataagtt ctatttagaa gattcttatt ttgaagttct aaatacaaat gggggagata
    33661 ctactaatga acaatctcta gtccttgtat atactaatgg gaatgataaa gttttattta
    33721 caggtgatgc tgaagaaggt accgaaaaag aaatactacc taagcttgag aaagtagacc
    33781 ttttaaaagt agctcatcat ggttctcgta gtagttcatc tcaagagttt ttagataaag
    33841 taaatcctga atatgcggtt cttttagtag gcaagggtaa ttcatatggt catccacatc
    33901 aggagacaat gaataaatta aaaaaaatgg gagtaaaagt acatagaagt gatgagtgtt
    33961 cagatataat atttgaatca acaggtgatg gagtatttac aagttgtaaa gatggaagtt
    34021 ataataaagg cgttagagaa gatggaaact ctggaaaatc aaacagtaat acaagtaaaa
    34081 atgattcttt taaaaataaa caaaatacat ccaattcaga agaaatagtg tattttactc
    34141 caaaaggtaa gagttatcac tctacaaaaa attgtagtgg gctttcaaga agtaaaaaaa
    34201 tattatcagg cacaattgca gaaagtaaga aaaatgaccc atgtgatata tgttatggaa
    34261 aataacaaaa aaacaaccaa tttatctata aagcttctga actttaaatc atttaaaaaa
    34321 taaaataaat gcataaaaaa ataagctata atcaatagat aacacatttt atctatacga
    34381 atagcttatt tttttaagta ttttttaata gctatttaat aactctaaat acaccagtta
    34441 caatacctac aatttcaact tctgagtcat tgagcataat tggttccata aaatcatttt
    34501 ctggctgaag tcttattaaa tttccttctt taaaaaacct cttaaccgta gcagattcac
    34561 catttataag tgcaactact atctgagaat ttaaggctgt attttttttg tctactacaa
    34621 cgtaatcttc atctaaaatt cctgcattaa tcatactttc gccttttact cttaatacaa
    34681 aattgttact gccctttact aaactggcag ggaatggaat gtattcctct atgttttgtt
    34741 gagctaaaat gggttctcca gctgttattt gacctactaa aggaagctct attatttctt
    34801 gattagctcc agaaacatca tttactttac tacgttctaa aacttcaata gctcttggtt
    34861 tagtagggtc ctttcttatg tatcccagtt tttcaagttt atttagatga gagtgcacag
    34921 ttgaagtaga tcttaaccct acagcagtac atatttctct tacagcaggt ggataacctt
    34981 ttaatatgat ttgagacttt ataaattcca atatcaagac ttgcttttca gttagatcta
    35041 aatacataac atcactcatt tccaaacata ttttgttttt atttccatac actttatatt
    35101 tttaattata acataatcca tttatatctg caaacatttg ttcctgattt ttaatcgtcc
    35161 tcaatccatt caaaattctt gactatatca cgttttacat ttttcttatg agctgcataa
    35221 agctgagtag ttgtgacatt atcatggtca agtaagtttt gaacatcata tatagaaaat
    35281 cctgtttgga ttagagaagt tgcagccgtt gctcttaatt tatggggact atatccattg
    35341 tctctagtag tatcaagtgg aatagatgta tattttttta ccagtgttct tatagccttt
    35401 gctgtaattc tcttattttg aagagacaaa aataaagcat ctttttcttc atcatttaat
    35461 agttcatctc ttgttctttc gttttgcaaa taatctttta ttacatgttc acaagtatga
    35521 tttataggca ttaaaacttc tttcccacgt tttctatata ttttaaattc acctcttgaa
    35581 aaattaaaag aggagatatt gagttctcta agctcattta atctaagccc gtatgtaaca
    35641 aataatgcta gtatagcttt gtcacgtaat ttagtttttc tccaatatac cttttctttc
    35701 tctgtaagcc cttcacctgt ttctactgat tcaagcatct tagcaacttc atctatctca
    35761 aggcgcttaa tagcatctgg ctgaggttta ggaagtttta ttggattgaa tccatcagtt
    35821 atattattat ctatctgaga gtttctgtat aagaatttaa atagagtaga gatggatgat
    35881 ttttttcttg ctaaagctct attgttattt tcaaaaatca atgtgttttt ttctgtattt
    35941 ttgtaatatc tactgcaata atcacctaag aataaattta tatctcttga ttttattgta
    36001 ttaaaatcat cttctgtaat gtcttttaca caatctgcat ttgttaattc tttggtttca
    36061 atcaaatatg aacaaaaaaa attaatatct tctaaataag caagtcgtgt agaaactgca
    36121 acagaacctt ttaaatatat aaaataatcc ttcataaata caggaagttt attttccaaa
    36181 agtagacatt tttctatgag ttcattttct ttgataacaa tattatcagg tttatctgat
    36241 ttattatgta cttttataat tttcttatca ttattcaaaa ttaaacctcc taaaactaaa
    36301 atataaatct tttatattat tttttgatat ttataccaag tgagtataaa tatttaattt
    36361 aatcattttt tacattttta agagattagc agtgctttag atgaaatcat cactaactat
    36421 tccctaaatc cacatttaaa gactagttga atttaagtac tcttttttat ttctcaatat
    36481 atttatagaa atattttaaa atatattgag aaatttttta tgctctacta aaatcttttt
    36541 tatataatat ttttatatgt ttctaacgaa accaaatatt agtaaaatat gtatttattt
    36601 aactaaagtt tttaaatggg ctgactaagc ttaaaatata ggatattatg agagttacta
    36661 ttgaaaaaat aaaccctatt gccataaaga aacttataaa tatgttatac ttaactatta
    36721 aactggatat attcaagtta tcaaatccaa ataagtatgt aattgaactt attacaaaaa
    36781 taactataac tataaagctc agaccattta agctattttt taaatcagca ctactaagtg
    36841 acatatgtgt agatatagaa tacattaaaa acataaatat aaaatacttc aatgaatata
    36901 cactaggtgt taacaacatt attcttataa aattggaaat atcacctaaa ataatgtgta
    36961 taaaaccatt tatatttaaa ttattaatca tacttacata taactttaaa tttatatttt
    37021 gagtcacttt catataacta tctggcagta atatcttaaa aattaaaata atcattattg
    37081 ttccacctat aataggtgca attcctataa agaaatttcc tatactttga taaagattat
    37141 cttttttata gctatgactt acatatccta gtataccatc atttttactt tctattggtc
    37201 tgaaaaactt aactgaattt attttatgca tgaaaattaa acacattatc aaatgactca
    37261 attcatgaac aaatgttcct atagctccag ttacaataat tccagttttt ccaaaagatg
    37321 aaaagataaa tttattattt agattttctg taatgctaaa aatcaatcca aatacaataa
    37381 atattcctat gaaacaaaaa atatttatta cacttatttt taaaatttcg attatataaa
    37441 tcataagtta agtcctcatg taaaagtatt taactactct acttcatatc cattacagct
    37501 taaaatttta caagcatttt ctatatcctt ttcttttact aagatatagt ctgtatcata
    37561 cgtagaaatt gcaaatatgc ttattttgtt gtctgctaac aaactactta acttagataa
    37621 aattcctaca agtgaaaagt ctaaaggacc tagtatctta agtattctcc actccttttc
    37681 tgacttgaca tcagaaggaa tattattatt taaacataca acacttaatt catcatcagt
    37741 tctagttata gagtaaaact tctctgtatc tatccatgtt ggtattttac tgtcattatt
    37801 taatctacat actgcatact cttctaccaa taattttaat ttcataatgt atatttcctc
    37861 caatcttatt tgtttactta agattataca ttaaaagtta cataaaataa aacatataaa
    37921 gtaaaaaagg ttcactatag tgtcctattt ctatagccaa cctttaaaac ttaattaaat
    37981 tctaataaag agatatatta tctaggttca attataagtt taatggctgt tttcttatca
    38041 ccttcgattt ctatatcagt aaatgcggga atgcaaacta aatccattcc actaggtgca
    38101 acaaaacctc ttgctattgc aattgatttt atcgcttgat ttagagctcc agctcctata
    38161 gcttgtattt ctgcaacgcc tcgttctctt agtactccag ccaaggctcc agctacagaa
    38221 ttaggattag attttgatga aacttttagt acctccattt caacatctcc ttgttaattt
    38281 gttaaagtat ccattaccta aagtactaaa tcataattgc catttactaa ttacaatcat
    38341 catttaaagt atacttttat aaaaatatac ttctataatc gttttaagaa aacctttatt
    38401 ttttttaacc attttattat gaaaaaacta caaatttctt tatattctat aagcattttc
    38461 caatatttct aaatttttta tatctttgag ctataatttc ctctttatcc atatctttgt
    38521 aatgatttaa tctttctaaa atgtaagctt ttatttcttt tgaaacagct tctacatctt
    38581 tttgtgctcc tccaagaggt tcttttataa tcttatctat tatttttaaa tcatataaat
    38641 cattacttgt aagcttcata acatcacaag cttctttagc tttagatgaa tctttaaata
    38701 gaatactaga aagcccttct ggagaaatca cagaataaac tgaatgttct aacatacata
    38761 catcatttcc tactccgata cctaaagctc ctccactacc accttcacca attacaaaag
    38821 tgattactgg aacagaaagt ttactcattt caagtaagtt tcttgcaata gcttctcctt
    38881 gtcctctttc ttctgcttct agaccacaaa aagctcctga tgtatcaata aacgttatta
    38941 taggtctatt aaatttttca gcttgtttca taagtcttaa tgcttttctg tatccttctg
    39001 gatgaggcat tccaaaattt ctttttatat tttcttttgt atctcttcct ttttggtgtc
    39061 caactatagt aacattaaac tttcctatct gaccaattcc tccaaccata gaagggtcat
    39121 ctttaaaaag cctatctcca tgaagttcta aaaaattaga acaaatatgt tctatgtatt
    39181 ccaatgtcgt aggtctcttt atatctctac ttagtgttac cttttcatat gctgatagat
    39241 gtgaaaaagc atcttcctta agcttttcca atttctcatt aagtgaattt attttatcac
    39301 ttaaatctat attatttgcc ttagaaatag ttattaattg tttaatatca ttttctaaga
    39361 cttttatttt ttcactatta ttttcaatca aaacagttcc tcctttatgc cctatttctt
    39421 gtgcatagca agaatttgat ataatacttc cttcattttt cttctatcta ctatcatgtc
    39481 aataaaacct ttttcaagta aaaactcaga agtttgaaat ccttcaggaa gtttttgatt
    39541 tattgtttgc tctattactc ttggacctgc aaaacctatc aacgtatttg gctcagaaat
    39601 tataatatca ccaagcatag caaaactagc agttactcct ccagtagtag ggtcagttag
    39661 aactgatata tatggcaatg atttttcttc aagcttagaa agtgcttgag aagtctttgc
    39721 catctgcata agagatacca taccttcttg cattctagct cctcctgaag cagaacaaat
    39781 aattataggt aaattatttt cagcagcata ctctattgaa taagttattt tttccccaac
    39841 aatagaaccc atacttccca tcataaaatc aggattcatt acacaaataa ctgttttaat
    39901 tccatttatt cttccataac ctgtgacaac agcttctgac tcttttgttt tttccttata
    39961 agagtctact ttttctttgt atttaggaaa atttagtgga ttttgaaatt ctacatcaga
    40021 attaaactcc ataaaagttc ctttatcaat caataaattt attctttttc ttgctcttaa
    40081 gccataatgt cttgaacatt ttggacatac aaatgaattt tcttctatgt cctttctaaa
    40141 tacatgacta tcacaacctt tgcaataggt ccaaaattta tcatctacac tattcttttt
    40201 aaagttgtca tctaaagaaa tcgtaacata cttagattct tttgatgata aaaatttttt
    40261 tatcataatc tatttactcc ttataatgta gcaaattctt tcttcataaa gacatcttct
    40321 ataaatttgg tatgatgagt atcttctcta aactcctcac ttgttacaag tgccttttga
    40381 aattctacat ttgtattaat accttctaca ataatttcgt ctagagctct atatattcta
    40441 cttatacatt cttctctagt ttcagcccaa catattaact tacctatcat agaatcatat
    40501 aaaggtggta tagtatatcc tgcatataca aaagtatcaa atctaacacc aaaacctcca
    40561 ggtaaattta atgattctat cttacctggt gatggagcaa aattctttga tgaatcttct
    40621 gcatttattc tacattcaat agcatgacct ctaaaagtaa tatcttcttg tttaaaagga
    40681 attttatccc cattagctat agatatttga agttttataa ggtcaagatt tgttaccatc
    40741 tctgtaactg gatgttcaac ttgtattctt gtattcatct ctataaaata aaagttttta
    40801 tctttgtcaa ctataaactc aatagtacct gcaccgatat aacctactcc ttttactata
    40861 tctactgcaa ttttatgaag tttttgtctt tcttcatctg aaaggtatgg acttaaagat
    40921 tcttctatta ctttttgatt tcgcctttgc atagaacaat ctctatcacc taaatgtatt
    40981 gcatttgaat gtttatctcc aaatacttgt acttctatat gtctaggatt ttctataaac
    41041 ttttccatat ataaatcact attagaaaat gctgctagag cttctgaact tgccatatca
    41101 aaatttgaaa aaagttcttc ttcttttcta acgattctca taccttttcc accgcctcca
    41161 cttgaggctt ttatcataac aggatatcca atttctcttg caacttctaa agcttcttcc
    41221 ttagttttca aaacagattc tgaaccagga acaacaggga tattcaattc catcatagtt
    41281 tctcttgctc tagatttatc tcccatcaaa atcatttgtt ctcttgttgg acctataaaa
    41341 ataatattat tatcttcaca ttgctttgca aattcaggat tctctgataa aaatccaaag
    41401 cctggatgaa tagcatcaca ctttaattta agagcaagat agattatatt ttctatatta
    41461 ttataactct tacttatatt atttggtcct atacaaatag attcatcagc tatatatcta
    41521 tgaaaacaat ctttatctat ttcagaatag atagcaacag tttttatacc tagttcttta
    41581 catgttctga tgattctaac tgctatatcg cctctatttg caactagtat tttttttatc
    41641 aacttaaata cctcctacta caatggtttt atcttaaata aaggttgatt atattctact
    41701 agttcttcat tactaactaa tacttcaatt atttcacctt taatttcact tgttatctca
    41761 ttcataagct tcattgcttc taatatacat aaagtgtcgc cttcttcaac tacatcgcca
    41821 actttaacaa agctatctgc atctggactt ggagaatcat agaatgttcc catcaaagga
    41881 gctactacta ctgatagatt ctcattagat gtgacaggtg tatcgtttac aactggcttt
    41941 tctaaagaaa gatccactac tgattcttgt tgtacactta aaacaggtga acttgtagat
    42001 tgagcttttt tagatacttc taatcttaaa tcgctacttt caagttttac atattctaag
    42061 ttagttgagt ctatggcctt taataattcc ttaatttcat tgatgttcat tttgttaccc
    42121 ccttgtatta ttacctggtg ctaatattat atcacaaaaa acaaatttat aaaagaaaaa
    42181 gtgctgacta gagaatcaac acatcaatat atcttttaat tttaaattaa caaccagtaa
    42241 cttatatttg ttttaatttt ccattattaa tataaattat aagtataaaa tgaataaaat
    42301 tcaatatttt ttgcaaaata caagagaata caaattaata cctcgggaat ttttaagaca
    42361 aataccaata ttatgcaatt tagacttcgc tttctttcaa attagtatca ttttctttat
    42421 ttttacatag gatgtacttt aatttagcta tcaaaacatt gctatatagg ttaaaagtta
    42481 ttagaattgc aggaattata aagtaaactc tagcacctat aaaaccaaaa actactgtaa
    42541 taagtattaa tcttttattt aaagtaaaaa aaggtatatt aaaaatactt ttgtcggatg
    42601 tttctggagt ataagaatat ataactccaa aaatcaaagt aagcatacaa agtggaagta
    42661 gtgtataact tgaaattatt cccagaaatc ctatactttc aaaatatttg ctagcactat
    42721 ttgtataaac aaacatgtat ccatttaaat atgcgcatat acatgatata atgccaaaag
    42781 ttccccctaa ggattgagtg taccaattaa aaaatttaga ttccatatac ctcacctcct
    42841 aaaactgtat atataatgat atgcatttaa atagtaaaat atatctttat atgtttctga
    42901 ataaaatttt gttaatttca ttaatttatg tttaagaaat ttgaagtata gtgtagtata
    42961 tattaatgta gatgaagttt tatagtactt atttcatagt gcttaatcaa gttatcttat
    43021 tatggattca aaagttgttt gtagggtttt atattttgtt gagctgaaaa atttattata
    43081 aatgaaggga tgttttcgct ttggtagata cattagaatt tggtcttaaa attttatttt
    43141 ttatattatc aataatttgg atgggtaaaa taatgatttt aagaactgat aagcaaatag
    43201 taatcaatcc tttgcttatt ggtatatctg cagtccttgt gatgttgcat acctcacaga
    43261 gtaacattga attttttgga ttagatgtcc aatatataag aatagttttg tatataatat
    43321 attcactgat aattttaata ggaatatggg ctactaacag aagaaatgga attttttaga
    43381 taatctattt ctataaaatt taaaggttgc tagtaacaaa tcactaatat tagcgttctg
    43441 ttactaacaa cctttatttt gattatacac ttaaattgaa aatagaatga ttgaataagt
    43501 tgacagatta ttttacattg acagtaatta gaacttcttg agatgtattt aaatcatttt
    43561 tgtcgcttag tataactttt accactgctt gacctgtttt attaccttta attatcatat
    43621 cttcacatga aagtacgctc ttatcactta cagatacaat aaaatttaaa tcttttggaa
    43681 ggttatcagt aacttcccag tatttataat aactatcaat tttcttcgta attatttttc
    43741 tcaaatcaat actttcacct tttgctatat caacattatg atatttagag tccagtttat
    43801 actgattatc ttttgtatca ataaaattta aatcgcaatt tacatccaca ccattatgag
    43861 aaagagaatt tactacatcg tcatcaatca aataattatt ggcaagattt attgaatcca
    43921 tatcttttag acttaaagct tccttaggaa cctcttttat attattattc caaaaaataa
    43981 aattgtctac attttcaaga tatgatattc cttttaaatt tgaaatgctc tcaccagttg
    44041 cataaaattc tccatctaaa gcctttatat tgcttatagt catctcatct gggtttatat
    44101 atctttttac aactgctctt aaatttgcat caggaaaaat atctttaagt tgattttcgc
    44161 tcaaaacctt atcatttgaa tttattggac ttaaagctgc tgcagaaata ataaatggta
    44221 ttaaattagc taaattcata gtaccctccc acataaattt tttattataa taatttaatt
    44281 tctatgagag ggttctgttt ccttgttata tatacctact ttcgtacact ttattaatat
    44341 aaattaaaat aaatttctac tattaaactt ataaacatat tactagttac gttttttagc
    44401 ttcacatcta aatattcgca taccttgtga cataaatttg tcttcatact ctgttgtaac
    44461 attattttca tactcactat ttcctaaatc aagagaaata ttctttaata gccaattatt
    44521 tgcagcaatt tcatttaaac taaactcgaa taatttttca ttatctgttt taaagtgaat
    44581 ttcgccatca tcttccaaaa ccctattgta catttctaaa aatccagaat gagtgagtct
    44641 tcttttactc catctatttt taggccatgg gtcacagaaa tttatataaa ttctcgataa
    44701 ttctttagct tcaaagtaat ccaaaatatt gcttacatct ccccataaaa atagtatatt
    44761 attcaaatta gaagcatctg ctttttcaac agcctttaaa agaacttctt ccttaagttc
    44821 catggcaata taatttatgt cagggttctg cttagctaaa gttgttataa acttaccttt
    44881 tccagtccca aattcaacat gtattggatt atcatttcta aactttaaat tccacttgcc
    44941 ttttaatttg tctatatcgt ctcttaaaac atattttgta taacttaaaa gcttttcatc
    45001 tgcacctttt tttcgtcttc ttctcacaat aattctcctt acatataaaa aattagtaaa
    45061 atacattttt attatacatt atctcaccaa aaaataaaaa gaactctctt aaaatagaaa
    45121 tttatttcta taaagagagt tctaaactta ctaaaaactt aatcttagtg gcaccctgaa
    45181 caaccagagc atcctcctcc accttgatct ttcatatttt caggaataac tctaaatcca
    45241 aatcctgtat cttctattat tatatctcca tactgactat attcatcaga gctcattaca
    45301 aattgtaatt ctccaacttc ataagttaag tcatcttctt ttttctcatc taaagctaat
    45361 ccaaaagaag gacctccaca accaacacct gcaaagtata ctcttatatt attaggttta
    45421 tcttgattgt cttttaagat actttttaaa gtatcaatag ctgtttctgg taaagtaatt
    45481 ttcatcaaat cattcccttt caaatatttt attaaaacaa tatctattat atacccatac
    45541 aaggtacata atcaattagt ttttgcatat tttactaaaa cttctaccta atattaccta
    45601 aggctttaaa
//
LOCUS       DAENCV010000018        45025 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAENCV010000018 DAENCV010000000
VERSION     DAENCV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.018"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(93..779)
                     /locus_tag="KQD82_000957"
     CDS             complement(93..779)
                     /locus_tag="KQD82_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379440.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(794..3271)
                     /locus_tag="KQD82_000958"
     CDS             complement(794..3271)
                     /locus_tag="KQD82_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4379441.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(3490..4893)
                     /locus_tag="KQD82_000959"
     CDS             complement(3490..4893)
                     /locus_tag="KQD82_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379442.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLKKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIIFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVHKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            5387..6247
                     /locus_tag="KQD82_000960"
     CDS             5387..6247
                     /locus_tag="KQD82_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4379443.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(6486..7043)
                     /locus_tag="KQD82_000961"
     CDS             complement(6486..7043)
                     /locus_tag="KQD82_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379444.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(7233..8120)
                     /locus_tag="KQD82_000962"
     CDS             complement(7233..8120)
                     /locus_tag="KQD82_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4379445.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            8239..9438
                     /locus_tag="KQD82_000963"
     CDS             8239..9438
                     /locus_tag="KQD82_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG4379446.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(9632..12187)
                     /locus_tag="KQD82_000964"
     CDS             complement(9632..12187)
                     /locus_tag="KQD82_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4379447.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(12184..12870)
                     /locus_tag="KQD82_000965"
     CDS             complement(12184..12870)
                     /locus_tag="KQD82_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379448.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     IVVNTGGEVL"
     gene            complement(12947..13855)
                     /locus_tag="KQD82_000966"
     CDS             complement(12947..13855)
                     /locus_tag="KQD82_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379449.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(13857..14528)
                     /locus_tag="KQD82_000967"
     CDS             complement(13857..14528)
                     /locus_tag="KQD82_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379450.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            14790..15854
                     /locus_tag="KQD82_000968"
     CDS             14790..15854
                     /locus_tag="KQD82_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG4379451.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            16012..16698
                     /locus_tag="KQD82_000969"
     CDS             16012..16698
                     /locus_tag="KQD82_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379452.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTVKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            16688..17719
                     /locus_tag="KQD82_000970"
     CDS             16688..17719
                     /locus_tag="KQD82_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379453.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIHVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            17801..18466
                     /locus_tag="KQD82_000971"
     CDS             17801..18466
                     /locus_tag="KQD82_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379454.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            18479..20896
                     /locus_tag="KQD82_000972"
     CDS             18479..20896
                     /locus_tag="KQD82_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379455.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(21077..21868)
                     /locus_tag="KQD82_000973"
     CDS             complement(21077..21868)
                     /locus_tag="KQD82_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4379456.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(21901..22488)
                     /locus_tag="KQD82_000974"
     CDS             complement(21901..22488)
                     /locus_tag="KQD82_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4379457.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIYNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(22665..24056)
                     /locus_tag="KQD82_000975"
     CDS             complement(22665..24056)
                     /locus_tag="KQD82_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG4379458.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(24246..25544)
                     /locus_tag="KQD82_000976"
     CDS             complement(24246..25544)
                     /locus_tag="KQD82_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG4379459.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIQNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(25903..26778)
                     /locus_tag="KQD82_000977"
     CDS             complement(25903..26778)
                     /locus_tag="KQD82_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG4379460.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(26971..27657)
                     /locus_tag="KQD82_000978"
     CDS             complement(26971..27657)
                     /locus_tag="KQD82_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG4379461.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(27706..28239)
                     /locus_tag="KQD82_000979"
     CDS             complement(27706..28239)
                     /locus_tag="KQD82_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG4379462.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(28283..28915)
                     /locus_tag="KQD82_000980"
     CDS             complement(28283..28915)
                     /locus_tag="KQD82_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG4379463.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLETVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(29005..30084)
                     /locus_tag="KQD82_000981"
     CDS             complement(29005..30084)
                     /locus_tag="KQD82_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG4379464.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(30217..31563)
                     /gene="pepV"
                     /locus_tag="KQD82_000982"
     CDS             complement(30217..31563)
                     /gene="pepV"
                     /locus_tag="KQD82_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG4379465.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTIAAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(31632..32912)
                     /locus_tag="KQD82_000983"
     CDS             complement(31632..32912)
                     /locus_tag="KQD82_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG4379466.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(33039..33299)
                     /gene="hfq"
                     /locus_tag="KQD82_000984"
     CDS             complement(33039..33299)
                     /gene="hfq"
                     /locus_tag="KQD82_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG4379467.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(33413..34348)
                     /gene="miaA"
                     /locus_tag="KQD82_000985"
     CDS             complement(33413..34348)
                     /gene="miaA"
                     /locus_tag="KQD82_000985"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG4379468.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(34386..36353)
                     /gene="mutL"
                     /locus_tag="KQD82_000986"
     CDS             complement(34386..36353)
                     /gene="mutL"
                     /locus_tag="KQD82_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG4379469.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKKSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYSCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(36364..39207)
                     /gene="mutS"
                     /locus_tag="KQD82_000987"
     CDS             complement(36364..39207)
                     /gene="mutS"
                     /locus_tag="KQD82_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG4379470.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(39368..40102)
                     /locus_tag="KQD82_000988"
     CDS             complement(39368..40102)
                     /locus_tag="KQD82_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379471.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIKVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(40071..41084)
                     /locus_tag="KQD82_000989"
     CDS             complement(40071..41084)
                     /locus_tag="KQD82_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4379472.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(41106..41876)
                     /locus_tag="KQD82_000990"
     CDS             complement(41106..41876)
                     /locus_tag="KQD82_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379473.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYYNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(41851..42141)
                     /locus_tag="KQD82_000991"
     CDS             complement(41851..42141)
                     /locus_tag="KQD82_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG4379474.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(42272..42371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      42991..43080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            43133..43309
                     /locus_tag="KQD82_000992"
     CDS             43133..43309
                     /locus_tag="KQD82_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379475.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(43424..43780)
                     /locus_tag="KQD82_000993"
                     /pseudo
     CDS             complement(43424..43780)
                     /locus_tag="KQD82_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            44036..44950
                     /locus_tag="KQD82_000994"
     CDS             44036..44950
                     /locus_tag="KQD82_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBG4379476.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNNLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVPPPLQQTYQKRLLNAFGILKKDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGHYIFTLHNKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKMLV
                     KVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT        15383 a         7027 c         5188 g        17427 t
ORIGIN      
        1 atagcacatt tatagtattt aacttatatc cactttacat atgtctaatt tatatagtta
       61 aaaatgctta tctcaaatac aataattagt ccttatacaa ttaaaccatc tgaaactgta
      121 atagtacgac tactactttt ggctgcttct ggtgagtgag ttaccataat tatagtttgt
      181 ccattgtctc tgtttatttt attcagtaaa tctattattt ctatccctgt tttactatct
      241 aaatttccag taggctcatc agcaaataaa atctctggtt ttccaattaa agctcgagct
      301 atagcaacac gttgttgctg accaccagat aactctcgtg gtgtatgttt acgtctatca
      361 gttaaaccaa ctatatctaa gatttcatct aattgatttt tataatcttt taaattttta
      421 ccatctaata acaaaggaag cattatattt tcctccacat ttaaatttgg tattaagtta
      481 taaaattgaa acacgaatcc tatatttcta cgacgtatta tgctcatttt ctcatcatta
      541 aaatgtgata tatcagctcc attcatatac acttttccgc tcgttggagt atctaaacca
      601 cctaaaatat ataaaagagt acttttacca gaaccagatt gacccatgat agaaacaaat
      661 tcacctttca ttacttgtaa agatacctct tttaacactc gagttgttgt atttccaatt
      721 ttatattctt tgataatatt ttttgcttct acagctaaac tattatgtgt aattttcata
      781 ttaacctcca taattattca aacttaattt cttctatcag tttcattttc ttccctttta
      841 taataggcac tattgaacct attaaagtta ctataattcc caataaccca acaataataa
      901 atgttttaaa atctaaatct ggtttcattg aaatttttgg tcctgcaact ataaagatag
      961 tttgaatctc aagtatagat atcacaatac caataacagc tccaagtaaa cctgaagtaa
     1021 acccttcaat caatgttact tttatatttt gtttatcact tagtcctata gatttataca
     1081 tagctataga acgtcttttt tgaatataat taatcagtag attattaata atacctactg
     1141 tagcaagaag aaaaatgaaa taggtcatct tattcatagg agacaaaaaa ctacttatag
     1201 tatttaaaga atcattatta aattcttcaa ctgtacgact ccaattatat gtatctccaa
     1261 ataaatatct tatctgaatc ataacagcat ctggattgac tgctgtatat actaaaaaac
     1321 cataattagt tttatcaaaa tcattaactg cataatgtga tggtataaca gcttctacat
     1381 catttgcacg ggatttaaaa ctacctacta ttttataaga aaaatcatac ttatcattag
     1441 atagagttat tgtatctcct atagataagc ctactttttt tagagtatct tcattcaaaa
     1501 taacagaacg tttatcatta aaagcttcta ttacttgttt ttttatctca aaatttgtat
     1561 aattgattcc aaacatagag ttatatagtt ttatattatc tgtaccctct agtcttgata
     1621 atgttacacc attaccagaa atctcattat tcataacata aagaggtaat atttttttaa
     1681 ttccatccat atgtctaaca tcttcaataa actcttcatt catctttcca tctgcaaaac
     1741 cttgtaattc tgcgtcacga aacacatctg ttatataagt tttgacaaag ttaccaacaa
     1801 cacttattgc aataactgca gaaatactta taaatagtaa agtgatattc tgtattatat
     1861 ttttgttatt tttcatattt ctagcagcta atttaccctc attccctaaa atatttttat
     1921 aaacaaactc aaaaactata gacatgatat cagtaataag aggaatcaac acaatagtag
     1981 ctacaattaa tcccagtaat gaaaatccac cagctagata caatgtattt tcaggggata
     2041 ttcttggaag taaaatagat aaaataaaca ttatacttcc tataaaaagt atagaacgat
     2101 ttgaaacatt cttttcttct actgttccta gtacaatatt ttttatcggc aaatggctag
     2161 cttttttaac aggtatatat gcactaaatg aagaaacgat tattgctact ataacagata
     2221 taatcactcc aatgggtgat attactactg gaattgatat tccttgctcg agagaatttc
     2281 ctaacccatg aagcattaaa tttaaaacaa ctacaccaat aggaatagca attaatcctc
     2341 ctatacttcc gtataatata ctttcaagca tcaatatacg agttacagtt ttttcattgg
     2401 caccaatact tctaaatgtt ccaataaaag gaagacgttc taatgtaata accttataac
     2461 tgctgtatat tataaatata ctaagggtta atgcaaaaaa acttattaaa aagaaaggca
     2521 ttgtcttttg tctagcatct gaaattatct tagtttcatt tatagtattt gataccatat
     2581 atttttcagt agaaagttct ttggataatt tatttactag attttctgta atttcttctt
     2641 ctgactctat aagaattctt gtatatccac tgcctttatt tataattttt gatagagtct
     2701 ctttgggtag cagtgcattt acacctcttg tgttcctcaa aaatactgta tcataagatg
     2761 caatatcact aacttgaaaa gtataagact ttccataaat ctgcaaagta atagaatcac
     2821 ctttttttat tttatatttt gatgtaaagc gatttggaag aataatttta tcaccagaaa
     2881 aatcagtaat actatcgcca ttttctaatc gtggcttgtt gattttattt agttgactta
     2941 agtctgctga tataagacta aattcttcat agtatccccc tttattataa atagcagagc
     3001 tttccaatac accaaccttt gattttatag catttaaatc aggtatgtct tctaaattgg
     3061 ttaatatatc tggattttta ctttggacag atatagtcgc tgtaccatac ataccttttg
     3121 ccatttttct ttgtgcactt tcataagata atccaattga gaatgaaaca aaaagtaaaa
     3181 ctgttgataa aactatggac aataacataa ctgctgttct aatttttcgt tctttaatat
     3241 ttgtgaaaat atactttaat ataattttca aatttacacc ttctttcctt atctaagata
     3301 tttcaatcta atatttttat actttaatct aaattaaatc tctaatagac tcctccatac
     3361 ttttatcatt ctatttataa tatttttaat caaatcaccc cttaaataaa aaagattgat
     3421 aaactactat ataaatataa taatctatca accttaatat attttttatt taaccttaat
     3481 taaccttaat taaataggaa aggtgatata aaatgagcta cctaatccag gtatactttc
     3541 aacagatata ttccctccat gaacttcaat aatttgcttc gttattgcta gtcctaaacc
     3601 agttccttct attttttgtt ttgtattgct tcctctataa taacgattaa acaaatttga
     3661 tgcttcttct cttgacatcc cttttccatt atcagatata atgatttcaa ttctttcatt
     3721 taatgttata tgtacacaaa ctttagtatt tttatctcca tatgtaaaag cattgacaat
     3781 aagattacta aaagctcgtt ttaatagtgt acaatcaaaa tcaaagttaa tattatctat
     3841 attactctta aattcaacta tatttgattc atattctgga taatttaaaa tatcaatgga
     3901 taattctttc aaaaatctta ctaaatcata attattttta tttaaaggga ttattccatt
     3961 ttttaattga taggttaatt ttaaatcgtt tataagtgta tttgtatatt ctatgttctt
     4021 taacatgatt gaagcatatt tatgtacttt ttcaggtgaa taattagtac tactatctgt
     4081 aagaagctct gcataccctt ttattggtga aagtggagtt tttaaatcat gagttatatt
     4141 tgaaatccac tcttctctca taatatcagt tttattttgt atctcatcac ttttatgaat
     4201 ttcttcattt aaaatattta aactattata tatatcagca aaaacacctt taatattaac
     4261 tggaagatag cttcgttttg aaatatcgct aacagcagat gtaatatttg atatgcactt
     4321 tgttacccag taaccataag caattccaga aaccaaaatt attaaaaata taatccctat
     4381 gattgaaaat ataattgact taccacctat aaatttacct ccatcaaaaa acatattcac
     4441 tttagatata tttaatggaa aatatataat ataaatatag tcatttttat tatttttaat
     4501 tgtattaaag tatacagttc cattacttga attattttgg ttagctaatg ctaataattc
     4561 agaattagaa taataatctt tatgctcttt tggttctaaa aacccataaa ctctatttcc
     4621 actagaatct aaaatttgca accacaattg attttctcga agttttttta atccagactg
     4681 ttttacttgt ggcttattat ctataaatac gatttgttca ttaaagtttt ctgtaaattt
     4741 tttcggccag tcactacgaa taaattcccc atttggtttc tgaatagtaa taagtgataa
     4801 gaaaatacct attactgtca ggattaagca aataaaaatc atgaaaaaaa taatgtatga
     4861 atgaaatact gttttataac taggtttatg catttaatca actcttttct taaactttta
     4921 ttttgattgt tgcttatata aaatagacaa taattattga tatgtaaaaa tttatttaaa
     4981 aatacaacta tctacataaa ttatatttat ataattatat gttatcaagc tgtctgttgt
     5041 atttaataaa tcattttgat aacatataaa agtaagataa atttttccat caaatacctt
     5101 ataattatac acaaaatctc tatatacgtt aaacagtata gagaaaattt tcatagaacc
     5161 atctttatag gggatacttt ataaaagaat aaaaaaatta taatatataa aaaacttcat
     5221 aggagtgtaa caacatcagt aaagatatga aaacactata taaaaattca aaaatattaa
     5281 gaataatgaa atacttgaaa ttgcttatat caagaaattt tatataatat aaactggtca
     5341 aatatgttat aatattaaat tgtgacaagt tcatggaggt gtttatatga aaaaacaaaa
     5401 gtcgccaatt ttacttgaaa tgcttggact attttttggt tgtatatcaa tgagtattgg
     5461 tataaatatg tttttaaaac cacatacaat tgctcctgga ggacttagtg gtctatcatt
     5521 agtactaaac aaggttacag gacttccagt atcagctatc atgttaatta taggtgtccc
     5581 attagtaata cttgctttta gaattatggg tacaaaaaat tcattaaaaa ctttatttgg
     5641 aacagttgta ttttcagcta tagttcaatt aacagaccca ttatcaaaac taagatttac
     5701 taatgactta ttattgtctt ctattgcagg aggaatccta gttggtatag gtttaggaat
     5761 aatgtttaaa tcagatgctt ctactggagg aacagactta attgcactca tacttagtaa
     5821 gaaattccca ggaataaaag ctacaaaatt catgtcatgc ctagatggaa tggttgttat
     5881 atcttctggt atagtaaatc gtagtttaga aactgctctt tactctggta tagcactttg
     5941 tgtattaata aaaatagcag atatgataat ggaaggattt gaccattcaa aagcattttt
     6001 tattatctct gatgagcctg aaagtttaag acaagctata actgaagaac ttgatagagg
     6061 tcttactatt cttgatggta aaggtggata cactagagaa aataaagaag ttctattagt
     6121 cgtagtttct aaaaaacaag aactttatct aaagagatta gttaaaagaa ctgaccctac
     6181 tgcatttgtt attgttacag atgtacatga ggttcttgga gaaggattta aaaatttaga
     6241 gaactaataa aaattaatat tttaagtata tatactattt taattaaaat acgatttatt
     6301 caaaaagagt gtctcataaa ctttttagtt catgagacac tcttttttgt ataaaatcaa
     6361 atgcattttc agtattttaa caataaaaac aggattatct atattttgag gcaaccactt
     6421 tattgaaatt ttatttagaa aacaatcatt actaaataac cagcatagat attaaatgat
     6481 tatatctatt gttttttcaa ttcatattga taacaaggaa cattttttac agtatttcca
     6541 gtatgaattt ttttgatata ctttcctgta catttaaatc ctaataaatc taatgatttt
     6601 gtagaatttt ggttttcttc ataggttatt gcaattattt tatttaagtt tagttctcta
     6661 aatccatact gtattactct ttttgttatc tcagtagcaa aatcatgtcc ccaatagctt
     6721 ggatttatac atatacctat ttctgcttct aatgctttag gatttatatt attaaacccc
     6781 atatttccaa taactttatt gtctctttta tatactatag caaagtggcc aattttccct
     6841 tttttatatg gatttagaaa aaatgatttt ataaactttc ttgtatcttc taaagattta
     6901 tgctcactaa atggtaaatg ttttactact attttatttt tataacagtc aaaaaaatct
     6961 tcaacatcat ctaatgatgc aggtcttagt atacaacttc tagtttctaa ggttttcatt
     7021 tgaggatatt cagaatcaga catttctaat ccccctttat acattaatct aaatatactg
     7081 ttcatattta aaatacgata ttaattatat aaatgttctc aaattaacct tatttttatt
     7141 tttaataaaa actaattact atatgaatct ttcaaattta caaatttatc tctattttta
     7201 tattaaatta cttgtgaaat atgtattcta aattatatat tattagatat catgcttaaa
     7261 aattctttgg ttgctaagga taaagatata tcttttaaat ggcaataacc tatatttctc
     7321 ttaggtattg gctcttttat atctaattta aacaatttct cattttccaa ataatctatg
     7381 ctaaattctt ctataacaca agacactcca agatttatat acgcaaattc taaaagtaat
     7441 tcatgagaac ccaattctat atccgggttt agttttatac cttttgataa aaaatattca
     7501 tttacataaa gtcttgaatt tgctttattt tctagcatta ttaaaggcaa tgtgtttaat
     7561 tcttccaatg atattttcct acctttgtat tctatgtaat catttgcaca tacaaatata
     7621 tcatgtattt caatacattc aacaatattt agtgtatcat cttctattgg catgtttata
     7681 attgctatat ctatattccc atctttgagt aaagatataa gttctctact cgttctattt
     7741 attgtagata catgtatatg tggatacttc ttatgaaaaa tttctaaata ttttaacaag
     7801 aaaaaacgtg cagctgtatc tcctactcct attttcagag aaccgtattc taatttttta
     7861 aatttattta ttctatcttc tcctgatgat attaacttta atgcagtagt gacatgttca
     7921 cttagaattt tcccctctgg agttaatttt actcccttat tgtttctata aaataataaa
     7981 gtatctaatt gttcttctaa ttgctttatt gactggctta cagctggctg tgacataaac
     8041 aattccttag cagcaagaga aaaactctta ttatttacaa ctgcattaaa aaccttataa
     8101 agttcaagtt ttatattcat atataattac tccttatgtc aaatattagt attatttatt
     8161 ttacttataa ttatatttga attataatat acatggtact aatatgacaa tattaattct
     8221 ttaggaggat tttagataat ggatagatta gttggaactg tttcaagagg ggttagagca
     8281 cctattataa gacaaggaga tgacttagtt aaaatagttg ttgattctgt tttaaatgcg
     8341 tcaaaaagtg aaaactttga agttagggac aaagatgtaa ttgccgttac agaagctgtt
     8401 gtagctagag cacagggaaa ttatgctcac gttgacaata ttgcaaaaga tgtaaaagat
     8461 aaatttggag atgatactgt tggggttata ttcccaattt taagtagaaa tcgttttgct
     8521 atatgtttaa aaggaattgc aaaaggatgt agaaaagtcg tattaatgtt aagctaccct
     8581 tctgatgaag ttggaaatca cttaataagt attgatgaac ttgatgataa gggaataaat
     8641 ccttggtcag atgttttaac agaagaaaaa tacagagaac tatttggata taacaagcat
     8701 acatttactg gtgtagatta tgttgattat tataagaatt taatcaagga ttgtggtgca
     8761 gaggtagaaa tagtatttgc aaacaatcca aaaactattc ttaactatac aacaagtatt
     8821 ttaacttgtg atatacatac aagacaaaga acaaagagaa ttttaagaca aaatggagct
     8881 aaaaaagtat attcacttga tgatataatg acagcaagtg tagatggtag tggttacaat
     8941 gaccagtatg gacttttagg ttcaaacaaa gctacagaag aaacagtaaa attattccct
     9001 ataaattgtg atgaagttgt aaacaaaatc caaggtaata taaaagaaat aacagggaaa
     9061 gatgtagaag taatggttta tggagatgga gcttttaaag accctgtagg taaaatatgg
     9121 gaattagctg acccagtagt atctcctgct tatacaaaag ggctagaggg aactccaaac
     9181 gaagtaaaat taaaatactt agcagataat gattttgctg acttatctgg tgatgagtta
     9241 aaagaagcaa tttcaaaata catagttgaa aaagataata aatctgatga tttaacagga
     9301 aacatggttt ctcaaggtac tactccaaga agattaacag accttatagg ttctttagca
     9361 gatttaacat ctggaagtgg agataagggt actcctatta tttacataca aggatatttc
     9421 gataactata caaaatagta tgttttaata agatttaaat tttttggatt atatatatta
     9481 acatgaaaat ttattaaaac ctcttaatag atatgacttt tcaagagtta tatctattaa
     9541 gaggtttatt taatgttcac agataagata tatttaaata atttaaacaa gattgtcaga
     9601 aagttatata atcttaaaaa tttatataac tctaattctt atttatttct gaaataatat
     9661 cactcttcaa aatttgtctt actggaataa ttacagcaac tatactaata gtgatattta
     9721 aaataatcat acctatgtat agaggtgtat ttattttaag aattggattt atatataagt
     9781 atacacgttt taccataaaa taaatcataa attgtccaat aacacttcca attaccatta
     9841 taatacttgc atagaatcca tataaaaaac cttctcttat catcataagt aaaaacttat
     9901 tatccgtaat acccatagct cttaaaattc caaactcatg tcttcttgaa aaaattaagt
     9961 aacttactgt attgattata tggaacaaac ttattgcaag aagtacaaat acaataccat
    10021 atataaataa gagtttttga tttaagtagg tattattttt ttcaatagca atggtataat
    10081 ctgttacaat acatcttttt acatcactta caacactttt tatgttctca gacaattcct
    10141 cactgttact atcttctgtc ttttttatac ttactgaatt ataattttcg atatcaaagt
    10201 tatttttcat ttgcccattc gtcataacta tatccatacc ataatcacca atgaaatata
    10261 tgttatttgc catcacacgt tttacagtag cagcaactac aaattctttt gttgtataat
    10321 cattcttatt ttcttcttta aatttaacaa tttcatctgt tggtttcatt gtctttggca
    10381 ctttaacttt tattgtatct cctggattta tatcaactgc atcatacatc ccagttccat
    10441 ccataggtag tctaactata accttattat ttttaaccat atcatgggta ttaatctccc
    10501 catctataat ataagaattt aatccttcta acataccatt gttataacca taaatatttg
    10561 tttttaaaag atagccacct tcattttgct ttgtacaaat accatcaaaa aaagtcttta
    10621 tcctgtaatc tttttcaaga ggtttaaaga aatttttcca aaatagttgt tcttctttaa
    10681 tttgcatagc tccaaataaa tatcttactg gatagacttt tgatattccc tcaacattgt
    10741 ttaatcttga aattttatct tttggtatac ctacatcaaa atctgttgtt tgcatagtta
    10801 tttggtagtc agaatttaaa ccatcatcag ctttcattgt aagttcattg ttatttttag
    10861 taagctgtat agagtaacta gaacatagaa atataattcc accaagtgat aatgaaaaca
    10921 aaattccaat aaacattccc tttttttgag tcatgtattt atgagataat acttttgtca
    10981 tgttagagtg ttttacactt tctaatattc tctttttctt attagtaatc ttaaagtttt
    11041 tcatagattc tatactagag tatttattca actgatgtac aacacgtaca gcaattaaaa
    11101 ttaacaacaa taagataaat aaaaagccta aataaataga tttttcactt ataaaaaatg
    11161 ctcctggaac aatatcacta ctcaaaaata tatgtggaaa ttgttcatat attgtagacg
    11221 ctactccaat acctaagaag caacctatag gaaatccaac aataaaaagt aaaaataact
    11281 ctaaaaatag tagaaataaa agttgtttat tattagcgcc taatacttct aaaaccccat
    11341 attgtgatat tctctgaagt attgaaacat ggaaaatact atatactgct gctgcaccaa
    11401 acactccaac aagcaatata attgcatttg tatttagttc taaatttttt aagaactcaa
    11461 atatattata tttttctata ttttttggtg gttttactcc aaattcaact gaaagaggcc
    11521 agtttatata agttttttct tttgatatgt tgaatttttt cataaattta taattttgct
    11581 ttttaatatt tttattttca tcatatttta cataaactat ataagaatcc ttttctttag
    11641 gctgaaaata atcgtttaca aatccctcca gagcagtttc tgggtttgaa agacttgata
    11701 aaataccaca taacttatag attttaccat ctaaattgat ttctgttcca atttctttgt
    11761 ttacatttaa attgtttaga gaatatgtgt caagtactat ctctccatct ttactaggaa
    11821 gagttccttt taataatttt ctacctgtca tatctaaata tgaagaatca gcatttaaaa
    11881 attctataat aaaaggttca tctatctgat ttttaggttt tgtaacgcca agtctatcta
    11941 cagtgtattc ctttgatttt ttattttctt taattttatt aagttgttcc ttatctactt
    12001 tatagaaata atgataatct ccactatctt cccttatctt ttcaattcta cttttgtctg
    12061 cactatgaac taaagaacca attcccgtca ataatgctac tgatagtact atgcttaaaa
    12121 ctatagatat agattgtttt ttataatgct ttatatattg aattgaaaat tttactagag
    12181 acatcatagt acttcaccac cagtattcac cacaattcca tcttcaatat ggataattga
    12241 gtcgcatact tgtgctatat tttcattgtg tgttaccata agtatcgttt gatggaattt
    12301 tgcactactt tcttttaata aacataccac ttccattgta gttttactat cgagatttcc
    12361 tgttggttca tctgctaata taagcgcagg tttatttgcc aaagctctag caattgctac
    12421 cctctgttgt tgaccaccag atagttcgct tggaaatttt aaacgcttat cccaaattcc
    12481 caatgtcttc atcagcattt caatatattc atggtctata tggtttcctt tatctaatgt
    12541 aacaggaaga gctatattat cataagtgtt taatacgggc attaaactgt agttttgaaa
    12601 tataaatcct atatttcttc ttctaaacac tgtcaaatct ttttttccta ttttagatat
    12661 gttctttcct ttaattatta catcacctga agttggatta tctaatccac ctataatatt
    12721 taacaatgtg ctttttccag aaccagaagt tccaactata gcagtaaatg tatctcgttc
    12781 aatttctata cttactccat ttaaggcttt tactttagtc tcaccttttc catagtgttt
    12841 tactaaatta ttcgtcacta atatactcat ttaatttctc ctttcaatac agatatatgt
    12901 tctataaaat attgtaagtg acgataatta cactcatcct acaaaattag tttaaactct
    12961 tacaattctg taagaagata ctaaaacaac atccacttcc tagtttagat tctactataa
    13021 tgtttccatt ttctttttct aaaattaatc ttgtaagata tagaccaatc cctgtgccat
    13081 tttggttttc cacattttta cttctgtaaa atcttttaaa aatatcattt agttctctac
    13141 tatcaatacc tattccctca tctttaatag taatcttcgt atatagttcc atttttatta
    13201 cagaaatagt aatcgtgcta tctgatggtg agtattttat tgcattttct aaaacattta
    13261 caattgcttc tatggtccac tttttattat gaactaattt tatgtctgaa aatggttcga
    13321 gatttatcct tatattttta gcttctgctt tattaaagat agtactgata ctttgaataa
    13381 gagtatcttt aattagtagt tcttctactt caaattctat aactccatcc tctaatttag
    13441 tcattttaaa taaggattgt aacagccagt caatcttaaa agtttcattt ttcatctttt
    13501 gtaaaaactc cttcttctgt aatgtggaaa tatttatatt ctccagaagt tcacaataaa
    13561 tggttatatt tgaaagtgga gtttttaatt gatgtgacat atttgatatt aaacctttaa
    13621 tcgcttcttt ttctgacttt gactgttcta cctctatttc tatcatatcc ttaatttttt
    13681 ttacttggtg agaaattttt gacgctcttg tatcctttgt atctggataa gtaacttctt
    13741 taccttccaa tatttgattt aacatcaaat ttaattcatc atatcttctt tgatttattt
    13801 tagtagatac tataaatata acaactgtac atattataaa ggctaatata aacatattat
    13861 tctacctcat tccataaata acccattcca taagctgttt ttatatattt tggattagat
    13921 gggtcatcct ctacttttac tctaagtctt cttatattta cagatacaat attatcatca
    13981 acataatttt cttcgctgtc ccaaatatgg tgtaaaattt gttcctttaa taatatttga
    14041 cctttatttt caattagata acttagaagc ttatattcta cagagctaag ttccaaacat
    14101 tctttatttt tatatacttt tagtaacttt tggtccaatt ttattccatt ggaatatatt
    14161 actttattat tcgatttttt tcttattgct actttgactc ttgcctttaa tacagataaa
    14221 gaaaatggtt ttgtaatata atcatcaagc cctatttcta atccttttac ttcatcaatt
    14281 tctgagtctc ttgcagtaag cattataatt ggtatatctg acttttccct tattatttga
    14341 catacatcaa atccatctcc atctggaaga ttgcagtcta agattataaa atctatattt
    14401 ttactattaa atatatttaa tccttcttgt tttgtataag ccttaaatac attaaatcca
    14461 tcattctcaa atgcataaaa aagaccttca tttaagttta tatcatcttc aataattaat
    14521 aagttcaatt ttactctcct ttagattttt tagataaata attattttct tgcattatca
    14581 tactaaaaat attttaattt tatttatttc actaaaaaga ttgtatttaa atttgttttc
    14641 aaaataaaaa catcataatc aagcatatag ttatatattt cccaatttaa ttacgcttaa
    14701 attgaaaata taactttatt cttatataat taaattatac aacaataaag ttatattttt
    14761 taaatatatt tttactaggg ggaatagaat tgaaaagggt tgatgagaga aatactatgt
    14821 ttgcaagagc aaattacaaa aaagatagta ctgcttataa tgactattat aagaaaaatc
    14881 ctgacaaaaa agaaattgat gatagtataa gaaatagacc aaatttatgt agtgaaggaa
    14941 caatgactta caatgagtta aactctccaa tggcaagtag tgcattcgat tttttatctg
    15001 atatcaaatc tttatgcgaa ggaaaagttt ctgatactaa agtagatgta gatgcaaagt
    15061 caatgacaaa aaaaattaag ggtcttgcaa agcagtatgg agcttctgta gttggtataa
    15121 ctaaattaaa agactatcat atttatacaa ataggggtag acatgaagaa aattacggtg
    15181 aagaaattaa tctaactcat aagtatgcaa ttgtatttgg atgtgagatg gataaagaaa
    15241 tgataaatag agctccaatg atatgtgaag ttatagaaac atcaaagtgc tatgtggatg
    15301 catctatcgt cggcatgatt ttaagttact atataagaaa tttaggatat gatgctagaa
    15361 atcatatgga tgctaattat ttagtaatgc ctgtttttat agctcgtgat gctggtttag
    15421 gagatatagg aagaaatgct atacttacga ataaagatta tggctctaga cttagattag
    15481 gtgttgttac tacagatatt cctcttttag aagatgaata tgttgatttt ggactagaag
    15541 atttttgtaa ggtctgtaaa aaatgctctt ttaactgccc ttctcattcg ctttctaatg
    15601 atattaaaat tggtgatgat ggaaaatata attgggtaat tgaacatgag acatgctata
    15661 ttaaatggag atatttaggt acagattgtg gtatgtgtat atctgcttgc ccatttagcc
    15721 aaaatctcga aactataaag aatactactt catttaaggg taataatgag cttattcaaa
    15781 aagctttaga tgagtatact tcaaaatttg gtaaaagaat atttatacct ggaaatccat
    15841 cttggttgaa ataattttat ttaataaaga atctgtacta gaatttatca taggacaggt
    15901 tctttattaa atatataaat ttttaaatac tttttctcgt ttataacaat tcagcaatgt
    15961 ttttagtcta taattgacat tataaaatat taaactaaaa ggagtaactg tatggaaaaa
    16021 atattagtag tagaagatga ttctatatta aataaaactc tatcatataa cctaattgaa
    16081 gatggatata taattacttc aaaatttaca gctaaatctg cactgaagtc tatttttgaa
    16141 tgtgaatttg atttaataat tttggatatt aatttaccag ataaaagtgg gtttgaatta
    16201 tgtaatgaaa taaaggaaaa ttacaatata ccaataattt ttttaactgc taatgatatg
    16261 gaatgtgata tgattaaagg ctatgaactt ggagctttgg attatataac aaaaccattt
    16321 aatatcaata tatttaaaca gaaagttaaa gcttttttaa atcatcttac agtaaaaaca
    16381 aaacaagatt actataggga tggatatctt gaaattaact tttctgagtt atctgccaat
    16441 ataaatggaa atcaaattat atttacacca ttagaatatc gtactttaaa actgcttact
    16501 gaaaatccta aaagtatttt aacaaggaaa gtacttttag aaaaactctg ggatatagat
    16561 gctaactttg tagatgaaca tactttaaca tctgttataa gtaggattag aagcaaaatt
    16621 gaaaaagaca atcttaagta tatcaaaacg gtatatggta tgggatatat gtggttaggt
    16681 gaaagaaatg aattttaaaa aattttcttt tagttctatt tatttaataa ttgctgttgg
    16741 tataattttt acagctattt tatttagtat ctcaatttat ctaataactg atgatttaaa
    16801 actaattgct ctattattaa tatatacaat agtactttgt ggatttatag cattaacaat
    16861 atttttactt cataaaaaaa tagttatatt ttgttctaaa atatgtcaca ctttagatag
    16921 tatgatgaat agtgaagaca atattgaaat tgaattagaa gaagaaacta ttttatcacg
    16981 tatcaatcac agattaatta gaatgtatga agttattcaa gaaaatcgca atagtattgc
    17041 tatagagaaa gctgatttac aagaactagt ttctgatata tctcatcaaa ttaaaactcc
    17101 tattgcaaat ttaaaaatga ttaatactac tctactaaat caaacattta aaacagaaat
    17161 tcaaaaggat tttttaatgg atatggaaaa ccaattagat aaattagatt ttttaatgca
    17221 atctatggta aaaacctcca gactagagac aggtataatc acactatcta aaaagaaaaa
    17281 ttcaatttat gaaacaattg ctacagcact tagtggaatt ttgtttgatg ctgaaaaaaa
    17341 gaatattgaa gttacagttg attgtgaccc aaatttatat ataattcatg acaagaaatg
    17401 gacttctgaa gctattttta atatattaga taatgctgta aaatacacaa attctaatgg
    17461 taagatacat gtagctacag aatgttgggt tatgtataca aaaatagata ttattgataa
    17521 tggaaaagga atatcagaaa gtcatcaagc agaaatcttt aaaagatttt atcgtgaaga
    17581 agatgtccat gacatagaag gtattggaat aggtctctat ctttcaagaa aaattatatc
    17641 tcttcaaggt ggctatatta aagttacatc agatattgga aaagggtcga ctttttctat
    17701 tttcttacct aataaataaa tttttaaata tttcatttgt aatgattctg caacattttg
    17761 gttttatact gtatttacaa gatgttaagg aggtattatt atgagtattt tacaagtaat
    17821 taatttaaag aaatattatg gacaaagttc aaacattaca aaagctcttg atggtgtaac
    17881 tctatctatt gaaaaaggcg aatttatttc tattgtaggt acatctggta gtggcaaatc
    17941 cacattatta aatatgatgg gaggattaga taatcctaca tctggaaagg ttattataaa
    18001 gggtaaggaa ttatctacta tgactgatga acagcttaca atctttagac gcagaaatat
    18061 aggttttata tttcaaaatt ataatcttgt tcctttactt aatgtatatg aaaatattat
    18121 cttaccaata ggattggatg gagagaccat agataaggat tttataaatg aaattataaa
    18181 tatgctttct cttgaagaaa aattatatga tatgccaagt aatctttctg gtggtcaaca
    18241 gcaacgtgtt gctatagcta gagcacttgt aacaaaacca gctattatac tagctgatga
    18301 accaacaggt aaccttgata gtagaactag ttcagatgta ctgagtttac ttaaagtgac
    18361 aagtaaaaaa ttcaaccaaa caattgtaat gattacacac aataatgaaa ttgcacaact
    18421 aactgataga attattcgta ttgaagatgg aaaaattgtt aactaggagg tgtagtatat
    18481 gtttagaaat aataatcgca catttataag aaaaatagct ttaaatgatt taaaaattaa
    18541 taaacttaaa acatatcttt caggtatcat tataatgatt tctacatgtt tattattaac
    18601 agtgacctta gtttcatata atgcctctgt agatttagtt aatgcatctc catatcatgc
    18661 tatatataaa tctgtagatg aaaaagcaaa aaatatacta tataaagata aaaactttag
    18721 tggcgtagga acttacaaat taattggaag taataaaaag acagattgta ttatgtcgat
    18781 tgtttatgca gatgatacag caattaaact tatgaatttt caaccattga aaggcaaact
    18841 acctacaaat aaaaatgaaa ttgctatttc agaaaaatat ttacaggaat ttggtttaga
    18901 taaagatatt ggagatagca ttaaattaaa ttattatgat gatattacga ataaagaagt
    18961 tcaatgtgat tttattattg ttggatttct agaaaattat tatcaagata atgccaagca
    19021 atactataca gtcgtttcaa atgattattt taagaatata gcttctacac ctttacaaaa
    19081 taaaagtaat acttttgacg agtcaagacc tgatactgta gatgtattag ttaaactatc
    19141 agaagaaaat acagagaaag attcagcttc aattaagaca cagttaaaga aaattggttt
    19201 atctttaggt attaagaaat ataatatata cttaaatgat aactatattg aatcaaattt
    19261 aattgatggt gagcagatta ttacagtttt agtagttgga atagttgttc ttttttctag
    19321 cgtttttgtt atatatagta ttttttatat ttcagttgtt aattcagttc agatgtatgc
    19381 taagttgaaa tcgttaggta tgacttcttt tcagttaaaa aagattattt ctctacaagg
    19441 aaatattttg tcaattattt ttattccatt aggggttata gcttcttgta taattgctta
    19501 cataattcaa ccattagcat ggcaaatgat agctgattta tttataattt taattttatc
    19561 actggtaatg tttttaactg ttagaatttc tttatttaaa ccagctagaa ttatttctaa
    19621 aatatctgct attgaagcca tgcaatatac agaaactaaa tttagaaaaa aagcaaaaaa
    19681 ttttagttat ataaatataa aaaatctagc attaaagaat atcgagtcaa atagaaaaaa
    19741 aaatttgatt gcattaatat cgctaagcat tagtggagtt ttatttattt caatcgctaa
    19801 tcttgctaat tcaatagatt tcaaaaaaca actatctcaa caatttgtct ataacgaaga
    19861 ttatataatt agcattaaga gtgataactt atatgagcat attacagaaa tccaacaaaa
    19921 taatccacta acagattctc ttaagaatga aattaaatct attcatggag taaaaaaatt
    19981 aattgaatcc aaatcaatta aaagtaatat tatagaacct tacatcaaag atagagacga
    20041 aaaacaattt attactctaa ttaaaggtat tacgcctgaa ctttcaaaat cacttgaaga
    20101 atatattgtc agtggaaaaa tagactatac acatttagat tcagatagtt taattataaa
    20161 taaatataga acaaaatatt atggattaaa tttaaaagtt ggagatacag ttacatttaa
    20221 tatctttagt ggaaatgata ttattaaaaa gaaaatgaaa attataggaa tcattgacaa
    20281 ttccaataca ggaggtatgt tttttgcttc agacaaaact ttagaatctc taacaccata
    20341 taatacaaat ttagattttt ctattttaat agataaaaag cattctaaag aagttgaaga
    20401 aaagttgtta agtataattc agaaaaattc aaatttaatg ctttattcat atgaagatga
    20461 ttatagaatg attactagag catttcaata tataattata gcttcataca tttttgttgg
    20521 tttaatttct tgctttggta tactaaatat gaccaatacc ctaataaaca gcgttttaat
    20581 tagaaaaaaa gagtttgcat tacttcaagc cgttggaatg acaagaaagc aactgcgaaa
    20641 tatgctttat agagaaggtc taaacataag tatcaaagct atatgtactt caagtatact
    20701 aggttatttt ggttcaaatc tactttgcac ttttataaaa gatgtaatta gattagactt
    20761 tattaatttt aaattttcta tatttactat tttaattttt tcattcgtat taattggtat
    20821 acaagtttta gtgactgaat tacttgtaag aaatatagaa aaaaaatcac ttacagaacg
    20881 cttacgttct cagtaagaat gtatataaaa aatagccatt ttttatacaa gttttaatat
    20941 gattttttaa aagctatagg gatagaaaat tttgacttta cataattttc tatccctata
    21001 cttgttttta ttttatacag atacacttac ttatatctcc gagcaacatc accagagaca
    21061 taaacaagca tattccttat tttctaaata tgtcaattgc atgacttgta acccctacta
    21121 aatcttcacg ttcacaagta gcaaggtgat attttaaata taatgcaaat gcatcatcat
    21181 ccatagagtc tactgcttca cgcatataca aagccaaacc atctgatgcg acataatgca
    21241 gtctagttac attaaatgca gacatcaaat catcaatgtt ttctttacgt acaagctcaa
    21301 acaaatcctt tggttcagac ttagcagcaa atgtctgtgg atcaatcaaa cctttttcaa
    21361 tatattcaga tacattaata tttcctctat ggaacccctc gtcaatgaga cagccatcag
    21421 atataacata tgcagcaaaa accacaccac caggttttgt tacacgaatt gcctcatgta
    21481 aagcttgttg tttatcttct ttgttataaa gatgatatag tggtcctaac agtaacgtaa
    21541 tatcatattt attatctggg aaaacagata aatccatagc attaccctga gtaatgctta
    21601 tattttcatt tgattgagta tttttatgga aaacctctat attatgttcg actaattcca
    21661 ctgcgtcaac atcatatccc tgacgtgcca gtgtgtgaga ataacgacca gttgcagcgc
    21721 caatttcaag cacacaatca cctgatttta tgtacttatc aatgtaatgc atagtagtga
    21781 gaaattccac cattccatgt tttaatgcaa gtcgactatc ttcatcataa gtattataaa
    21841 agtcaattaa gtattgtttt gtttccataa tttcacctct tctatattag ttaataaaca
    21901 tcaaatagac aaattccaat ctggtttttc ttgtcttata aaagctgtta atatattttt
    21961 caacattttt tcagcactat atctatctaa tgttaatttt tttgttgctg ttaagacagc
    22021 tatgccatga gtataaagaa ataaatctaa aaatattact tttgcacatt ccaaatctat
    22081 tccaatgctt tccgaaaaaa gtcttgcctt tttttcattg taaatttctt tataaaaatc
    22141 attcgcctct ttcatcatca aatctaaatc atttataaat aacaacttaa ataaatacgt
    22201 ttcttcttga gcaaattcaa tgtatgaaag cggaagcagt aaataagggc ttacactttt
    22261 agaattacga taattaacaa catactgatt ataatattca tatgcaaaag caagaaattc
    22321 attttttaat tcattcatgt tttcgtaaca agtaaaaatt ggtcgagtgg aacactgtaa
    22381 tttacttgca atactcctcg catttacagc ttcaaatccc acttctcttg taatatccaa
    22441 gactgtattc aaaatcattt cttttgttat ctttacttta ggtggcatat atgttcacct
    22501 ctccattaaa tttttataaa acacatatgt tatataacta tagtaacata tcaagaaatt
    22561 atgtcaagaa aaagctttgc atttttttgc aattcctaaa acttataaat aaaacatgag
    22621 ttgcaattaa tctattaata gataacagca actcagattt tcctttaaat actcttcttt
    22681 ttgtctaata tttcatatac acaatcttct attgaattaa tatctttttc ttttcttttt
    22741 ttagaatcaa ttaaaatgaa atacagtata aaacctacag caagtcccca tgttccgcct
    22801 ttagcagcaa ttacacctcc cataagtcca atcaatccct tctcaacacc tgtattacat
    22861 agctccattg atagctttgt acataaatat ccttgaacta aaagtgttac tgacaatgct
    22921 aatggtataa gaggtgtagc aatctgcgca attggataaa aacagataaa aattgctgaa
    22981 acccatacta atgtacccat gccactaaaa acagactgca tggcttcctt tccaaccttg
    23041 tacctttgag caacacttcc tgtaagtgtc gcagctaatg gaccgcacat tggtatatat
    23101 ggagaaaaga ttgccataaa aatatttctt atcccactta taacattaga cctatttgaa
    23161 ttgaaatcta tatattcatc ttttctattt tcactttcag aaagtactaa gttttcacct
    23221 gttataaaat caccaaaggc aattatgtat atcataaaag ccattggtat cccttgtata
    23281 aataacatta cacttggaaa acctattcca aaaggagata ctgtatttat cagattttta
    23341 aaatctacta tgtaaactaa attgtcaaaa ctaaatctag gaatttctat ttctttacta
    23401 aatataccta ctattaaact cactaaaata gctgatacta caccatattc tcctaaatga
    23461 aataaaaact tattcttaac ttttagagta tcaaatcttt cagaaaacat tacaaaacaa
    23521 gcaaccaaaa ttcctactgt tatagatata ggataagaat caaatctccc tttaggatta
    23581 aactctgata ttatagtcga tataccgaca cccattagta ttccaccttt aatagaattt
    23641 ggtatcttac taactaactt tccagctaca ccagttatac ccattaaaat aaatattatt
    23701 cctaaatcta tttgtaaagc tgttaatgct tgtattcttt caatgcccat gctgaacttt
    23761 aacaaatatg cagttgtaag agttatagaa gctgtaatcc agccaggtac aactgggtct
    23821 cccataaaaa catgcaaaca gtataatatt gagttaatga tagccatact ccatgccact
    23881 tcatagggta atcccaaaat ttgtgtacta aggccaattg tgcctaaact tgtcatactt
    23941 gatattatac cttgaatagc ttctggtttc tcaaatctgt aatgtataaa tggaattctt
    24001 atcttaaaca ttccaaattt tatatgaggt tgttcccttc tagcgagtct ttccataact
    24061 tcactccacc tttcaataac tcttataagt ttacaacttt ataattgttt ataaattatc
    24121 aatgtcatta aaattagtat ataacatgca aattatataa acaataaatt aaatctataa
    24181 aattgataga ttttgtgcat atatagaaag accgcttcat ttttagttac ttaaatcact
    24241 taatattagt tatttaaatc acttgataaa ggttttttat aaaattcaat agttctattc
    24301 catgattcaa actgtccttt tgaatttcct tctacagttc cacctgaaga aaaaatccct
    24361 ccactcatac tcatacataa agtctccttt agaggcataa tatatggaat tggtaacgga
    24421 tgacccatat tattgtatgt taataattct acgttatatg gataattatt ttcttcaaag
    24481 atttctaatg cctttaaaca agcttcataa ctattccata tgttatcttc ttttccagca
    24541 atcataagta tatctgcttt ggaattttct atttttattc tagcctcttt tctattctct
    24601 gctcttttaa gacttttctt ataagtacta ttaaatccaa atggaatacc ttttgatata
    24661 ttttttttct gttcttcaaa gaatatatca ttgtctactt tgatatatgg aaatggctct
    24721 cctttatatg accatgatga agtatcattt ccactcatca atccattaag agcttggaaa
    24781 caataaatgt gaggttgtga tactacaacc tttttaattt gttcatatct tgaagcaagt
    24841 aaaaaggcca attctccacc ctttgatgtc ccatgtacaa atatttcttt agtactggta
    24901 atttcatttt cactaatcca ctcaaatact ttttcaaaat attctaatgg aacctcttct
    24961 aatttatctg gtaacccttc taaagcaaag tatgcaactg ttagtacatt gaatcctcta
    25021 gatgcaagtg gagctgctaa aaggtctaga gcgtctaaat ttccatcaga accacctagc
    25081 attaaaattg ttttattatt agaattttgt ttataaaata attttcctat aaactcatca
    25141 gatatgtatt ttgtggtaat atcttctgat ttaaatattc tctttaattc aacttctttt
    25201 ctttcaatag aagtttcaaa tatcatattc ataattattg gtttatcaat agatatattt
    25261 tctgcaaaat ttttaccttc agttttagat ttttccagcg aataaattaa ccccatacaa
    25321 ttatcagcct tataagtacc ttctataggt tcaactaaat ctaaatctac ttctcctttt
    25381 tcatttgaac aaaacactcc ataagatgaa aattcttctc ccttgcacca tggaaattcc
    25441 attttcacac ttattttaag tttagtattt tgaattaagt ccgaaaccat aatcttaagt
    25501 ctttcatcca caaatgattc tacattgtat acatttactt tcataatcta tttctcctta
    25561 taataattat ttatgtttta tataaaacag ctttatctaa tgcaacataa ataaattttt
    25621 aaattaagac accatattga ctatttcatt ttatcaacat attccatatt tgtcaacata
    25681 ttgtcttttt tagtaattta aaattatgta gtctatatga aatccagttg atttttggct
    25741 aattaaagat aggaatgcta ttaggtttag aatatatata ttcaaaataa aaaaccactc
    25801 atttaaagaa tggtttttaa tataaactat ttgttattaa taaggatatt tttttattta
    25861 aatttatata atcgaacatc taatatttaa tgtttaataa aattatgcat ttgcaaaagt
    25921 atatatggca ttaactcttg ctacatataa attctcatca ctacatccaa tatatctagc
    25981 atatttttca atcttttctt ttgcattttc aacatcattt tcatcgctct ccattaactc
    26041 atttatataa atatccatta atgacgcttt atattctctt atatcatagc ctaaaaattc
    26101 agtcaatctc tcaatttcct tactaaaatc tccatcttct tttaccaaaa attcgtatac
    26161 atcttcccta tatggattta aagttattat ttgttttact actttgcatt cgtcagcctt
    26221 cccaataaaa ccatctttat aattattaaa taaggcatta gattttttta tagattcttc
    26281 atcaatataa gaatgtaccg cagttatacg attttctata agggcatcta caacagcata
    26341 atgaatttta aaacaatcat tgtataaata attgctcatt ttctttattg tcgattctat
    26401 ttctaagaag ctcgcgtggc catttttttt atttttattt ggtataataa aagttttatt
    26461 caaattatta aaaagtctct taaaatctaa atatttttca caataaacac ttttaaaggt
    26521 atcgatatct atagttataa ttccaaaact tactatagtc tcaattcctt tcttaaaaga
    26581 ctcttctata tatttttctc ctaaacttaa acctctatca gatatttgct ttagattttg
    26641 aaaactattt aaacaatctc tctcaaagtt tttagatatt tccaaagcat ataatttaaa
    26701 gattttcctt atcttattat aattattttt tgtatcatca aatctgataa tagctccatt
    26761 tacatcaaaa gtaatcattg agttttaacc tccccattag tttcatcaat aaaataaatt
    26821 taatataaac tttataatca taatattaaa cttatttata ctataattat taatttgatg
    26881 ataaaagcta ttttccttct aaataaaaaa tttgtctaaa gtttctaaaa aatttagaca
    26941 aatcttaggt tattactctt taaattattt ttaaattatt ccaaatacgc caaataatat
    27001 tggtataaat atagctggta acaaattggc tacttttata ttcgttaatt taagtacatt
    27061 tagaccaagt cctataatta ataaacttcc agctgctgtc atttctgtga ctacaggatc
    27121 acttaaaaat ccatttaaaa atgatgctcc cacacatata ataccttcat acaaaaatac
    27181 agatattgca gaaaacatta cacctatacc taaagaagca gtaaatataa ttgaagctat
    27241 tccatctata acagatttta caaataaagt atcatgactt cctgtaagac cactttctaa
    27301 tgaaccaact actgccatgg ctcctataca gaataataaa ctagatgata taaaaccctc
    27361 tgctatagaa tcttttttat ttcctttaga aaatttactt tgtaaaaaag cacccaactt
    27421 atttacccat ttgtcaatat ctattaattc tcctatcaga gcacctacag ctacagatat
    27481 tattgtcact aatgtgtttt ttccttctag tgctcctgat ataccaatgt acaatacaca
    27541 taaagcaatt ccattcatta tagtatcgct tactctttga ggtaatcctc cttttattat
    27601 aagacctact atacatccac ctgcaattgc tagactattt gctattactc caaacatttt
    27661 ttctcccctc gattagctaa ttgaaattta tcctattatt aaaacctaaa catctatact
    27721 ttgtattgga tattttaaat attccaaaaa ttcttcaaaa ccttttctag atatatttac
    27781 agttttagta tttatgttag gatgaaaatt taagtaatct tctttaatta catctttatc
    27841 tatgtaaagc tcaacttttc tttctgtatc atttattaat gaaaaaggat ttacacttcc
    27901 aggaagaagt ttaagtacat tgtatagtct ttcaggtgaa gcaaacgata atctagaact
    27961 tcctatctta tctttaagtg agtttaaatc aacttgtttt tctcctttaa ctacaataag
    28021 ataatactta tttcctttgg catttcttaa aaataaattt ttacattgac acccctttgt
    28081 tattccatct aaaatgacta attgctctgc tgtatataca gcttcatgct ctactacatc
    28141 atattcaaca tttaatttat ccaaaatatc atatatttta gtttcattca tgctagattc
    28201 attttcatct tgttcttttt tattatttaa atcactcata cttttctcct taaacacttc
    28261 atcataatat cattgtgagg tattattcta ctatagctat tgctcttact ggagctccat
    28321 cagcatcatt atacttaaat ggtgctgcta taaatagaaa tttttctgga acagtttcta
    28381 aatttgtaag attttctatg attatttttc ctttttcaaa taaaatatga tgcacttcaa
    28441 atactgatgt gtcatatcta tccactgata gcatatctat tcctatgcct tttatattag
    28501 tattagctat atagtttgca gcttcttttg ttaatgtagg ataacctaca tagtactgct
    28561 ttttatccca aaattttgac caacctgatt taaatattat aaaatcacta ttttttattt
    28621 catcttcata tttttttaga tattctaatt ctatattctc atttcccttt tcaagttcca
    28681 atacataagc tttcccaaca aaattttcta tatccaactt atctagggtc tcacctttag
    28741 tatacatatg tcttggtgaa tccatatgag ttccattgtg agaaaataca ctaattaatg
    28801 tttcttgata accattttct tcaataatag ctactttctt gatatctggt ctattaggct
    28861 ctgcatatac aggcatgtca ttatgtgtca catgagttaa atcaaaaacc ttcatcttta
    28921 tataccccct atgtttttat tttctattat atacctaaat tcattttatt tctatcagat
    28981 tttattccat tatataccat actactatat attttttata aacctcaagg gatttaccga
    29041 aaaatcttgg aaagaattat cagtaaatat aacatcttca cccaaaaaac gtttatacct
    29101 tccttcttct ttctttaaag taattctctg tttttgtgaa tgattattaa aaaccattat
    29161 cttaagagga ggtttttttc tattagtctg cactcttttt aacatgtatt ttatgtgcat
    29221 atcagcttca ggaaatgaat atccacaaaa tattaaaagg tcagtatctc ttaaagtttt
    29281 ttctgtttca ttccaaacat tacttaaaaa tatgtttgac atattcttaa aatatgttgg
    29341 tggcactata ataggttctg ttaactcacc acactctaaa cattttgtct ctgaactatt
    29401 ttcaataagc ttcatgacac caccttcatg tggtgtaaga gttacactat tacaaatggg
    29461 gcaataaagc caattcaatg acccatgtat cttatataat ttgattaatg gttcttttgg
    29521 ttttctccaa tttaactcct ctttaaaatt tgtaaactcc acaccataat caagcataat
    29581 tggcaaatca ttcttatctc ttaatccagt aactgtattg tctatatgta tatcataatt
    29641 tgcactaata aaagtagtat caagaagcaa atcattttcc aaaagatttt caactaaaag
    29701 tttatgatat ttattatctg ttctaggtgt attatgaatt gcttcagcca ttagtagtat
    29761 taaatattgt cttaaaaaac gtatactatc acttctgtta tttagatttt ctagtccaaa
    29821 atttctaaat gcttcttttc tttgttctgc taagtctaaa agacctaaaa cttcttcaaa
    29881 ggtaggaaaa tttgcattat ctatattatc ttttactata tcaatattga acatctgttt
    29941 aaagaaaata tacaattctc tattcatttt ggtattataa tttgtgggat ttagtttttt
    30001 aaaatattcg ctaaaaagtt cattttgaat aggtagattc tctgctgctg aagctcctgc
    30061 tcctaaaaat attgctattt tcatatagtt cctcctaata taaatattac tcctattttt
    30121 atatccatta aatatccata tcatttattt tatgatacaa caataaagct gtcttttact
    30181 agaaaaaatc ctattacaag acagccttat attatattaa atctttaata attcgtaaat
    30241 agaaagagca tatatttgag tagcttttat aatatcctct actattaagt attcattagc
    30301 ttggtgttct gtttctggtt ttcctggaaa aatagctcca aatgcaacac aattatctaa
    30361 agctcttgcg tatgttgctc ctccagatga aagtggtgtt ccatcaagac cagtttcttc
    30421 ttcatatact tttctaagtg tcttaacaag aagagtatct tctggaacat agattgaatc
    30481 caagaaatca tattcttcat aatttaaatt gtatttatct gtcattttct ttaactcttt
    30541 tacaaaatca tcctttttgt atgttactgg aatccttaca tctattcctg caaattcttt
    30601 ttcgttatca atagttactc tacctatatt tactgttagt ttacctgata catcatcctt
    30661 gcaattagga attatattgt ttccattagc atcttcacca ataacttctg ctaaaaactt
    30721 aattatatta gaatctatac ctatattatt aagagctata caaagtctag ccactgcatt
    30781 tataccagta tcacttgctg ctgaatggac actctttcca attatacaga ttttattacc
    30841 ttcaacagta tattcaaaat ttaatttatc aagttctttt tttaatttat cagagtattt
    30901 tccaatatag attgctttac ctggaactgc atttaaagcc tttccaactg acaactctat
    30961 atcacttttg ctatcacaag tcaaatgaac ttgtaaaaga cctttctctg catttgttat
    31021 tgggaatcta gaatctggag taaatccata gtttggtatt tcttcattat tttctttata
    31081 tttatttata catctccaaa gattttcttc atcagcccca aaaatgaatc ttatcctctt
    31141 attaaagtct acatttaaat ccataagagc tttcactgca taaattgctg ctattgtagg
    31201 tcctttatca tcttgtgttc ctcttccata caatttacca tcttctaaaa cagcttcaaa
    31261 tggagggtaa ttccagcttt ctaaatcacc ttctggaacc acatcaacgt gaccaagtat
    31321 acctatcatt ttttcacctt gtcctatttc tgcatatcca taatatccat ctttgtatac
    31381 tgtcttaaat cctaaagatt tacataagtc taagatacct cttaaagctt tatctacatt
    31441 ttcaccaaat ggacaatttt cagttgcttc agatataaca ctaggtattt ttacactttc
    31501 ttgtattgaa gaaatcattt catcttgtag agaatttact ttttctttaa tttgctcttt
    31561 catattatca cccttcaatt ttcatcataa tatactaata aatattttga cctttaaaaa
    31621 aaaaaatatg cttatttttt cagttttttt ataaattcaa tcgcttttaa agtccccatt
    31681 tttgaatggt caaatgtaag tccaccttga aaataaacat tataaggagg tcttattgga
    31741 gcatctgcac ttaattctat agaagaacct tgtataaatg ctcctgctgc cattatgact
    31801 tcactttcgt atccaggcat gtcccaagga acaggtttta cataagaatc gactggtgca
    31861 gcttcttgta taccttcaca aaaacttatt acttcatcag cattattaag tctaatacat
    31921 tgaataatat cacttcttaa gtcatcatat tttggtaata catcatatcc taatttttca
    31981 aaagctcttg agcaaaagat agctcccatt acagcctgag atactatata aggcgccaag
    32041 aaaaaaccct gaagtacatt tctagttgtt ccaaaagtaa gaccacattc ttttcctatt
    32101 ccaggagaag tcattctata agatataagc tcaatcaaat cttttctacc agctatatat
    32161 ccacctgtca atgcaagccc accaccagga ttttttatta aagagccagc cataacatct
    32221 gctccaacat cagtaggctc tttggtatct aaaaactcac cataacaatt atcaaccatt
    32281 actatagctt ctggtttaac agactttatt acttctatag cttcttttat atcactaatt
    32341 gaaagtgatt ttctccaaga atatcctttt gacctttgta tcataacgag tttagttcta
    32401 tcatttattt tgtttttaat tccttctaaa tctaagtttc catcctctaa aaaatctaca
    32461 tcatcatatg taacaccata ctctttcaat gaaccttttt cttctcttat gcctataact
    32521 ccctctaaag tatcatatgg tctaccagta acagataaaa tttcatctcc tggtctaact
    32581 ataccttgta tacataaagt aagtgcatgt gtaccattta caattattgg tcttacaaga
    32641 gcatcttctg tattaaatac tttagaataa atttcctcta ttttttcacg accaatatca
    32701 ttatatccat atcctgtagt ccagttaaag tgcatatcac ttagtttaga ttcttgcata
    32761 gcttttaaaa ctttatattg attatattct cttatttctt ttatttcttc aaacttatct
    32821 tttatttctt ccattatttc ttgcgatagt ttaaaagtat catcatctat tccatagtag
    32881 tcttttaata attcttttgt ctcattaagc attttatcac ctgtctcttt attattttat
    32941 ttaaacatgt aaaggagact attttactaa aacaaataaa ttatagttta taagttagta
    33001 taagtctcct ctcaatcatc ttaaattaaa ttaattatct atctgttgtt attattattg
    33061 ttgttttggt tttgatttgt tagattaata tactttccag gttgtatagt tgatacagca
    33121 tgtttataaa tcatgttttg ttgtctatta tccccttcta ttaatattat ataactatca
    33181 aaccccttta caagcccttt aacttgcact ccattaacta aatatatagt aacaggtatc
    33241 ctttctttcc ttgcattatt taaaaacaaa tcttgtaaat ttaaaactgt atttttcatt
    33301 agatttgtac cctcccaaga ttaaattgaa cttctaaaat attcctacct tatattctat
    33361 ataagttatg acaaattttc aatattaagt tgtatactta aaaatttata tattatttta
    33421 tagaatcttt aatatataag attatttcat tttttaattc gtctatattt tcatattggt
    33481 ctaaatcaaa ccattttgct gttttatatc ttttaaacca tgttatctga cgttttgcat
    33541 accttctaga atctctttta attatttcaa tagcttcttc gtatgtatat tctccatcca
    33601 gatactttaa tatctcttta taacctatac cttgcattga tatcatatct tttttaaatc
    33661 ccatacttaa aagttttttt acctcttcaa ccaatccatt tttaagcata atatcaactc
    33721 tcatatttat tctttgatac aaatgttctc tatctctatt tagaactatt attattggtt
    33781 gatattcttc attaaatttt aaatcacttg aaaaatcatt cattttctta ccacttagac
    33841 aaacttcaag agctcttatg acccttttag tattgttttt atggattcta ttagcagatt
    33901 cttcatcaag ttcttttaac ttattatgca tgtaatctat tcccttttca gcaagctgtt
    33961 tttgaagttc ttctcttaat tcattattgg catcagattg tgcaaaatcc atattatata
    34021 ttaaagagtt taaataaagt cctgtgcctc cagttactaa aacacatttt tccttttcat
    34081 ttatttcatg tatatatttt tttgctcttt gttgaaactc tgaaactgaa aatggatagt
    34141 caggcttaac ttcatctata aggtaatgct tcactccttg catctcttcc tctgtaactt
    34201 ttgcacttcc aatatccata tattcatata tttgcataga atcagctgaa attatctctg
    34261 cattcatatc ttttgctaat tttattgaca aatctgtttt tccaacagca gttggacctg
    34321 taagtattat aagtggtatt tttttcataa cattcctttc catatatttt aaatatccaa
    34381 atattttaca taattctttt aaacattttt tctatttctg tcttagatat ttcgaccatt
    34441 ataggtcttc catgaggaca tgtaaatgga ttctcacact tttctagctg ttccaataaa
    34501 ctttttattt caatatcgta tattttatca tttgctttta tagcagacct acatgccata
    34561 gatgcaaatc tctctccttt taaatcataa ttacttgtaa tttcttctat attatcaatt
    34621 atttgaagta taaatttctc agtttcaggt actccaaata tagtaggaac acatcttacc
    34681 attatatgat tattgccaaa aatttctagc tcaaaaccaa atttcataaa taactccaag
    34741 ttattttcaa tttgaagcat atctacattt gatacttcaa taaccactgg gtctaaaagt
    34801 atttgcatat ttatatcttg tctatagaac ttctccatat atctttcata tagtattctt
    34861 tcatgtgcag catgttggtc caacaaatac atagaatcgt ctttgctcaa aattatataa
    34921 gtattaaata caactccaat tacagaatat ccgtataaag aaaacttgcg ttttgaacta
    34981 cacgagtatt cttcttcact gtccttaata ctatctccta agtaataatt tccttcattt
    35041 ttaattccat caacttgaaa ctcttcttgt atattatcat ttgcactatc ttcacttaga
    35101 taattaatgt tttttacatc atcttctaca cttgcattta ggacttcact tactgattga
    35161 aattcattta ttggtttttc acttgataaa gtaacaactt caatgacttc atccttacct
    35221 ttagtaatat tactcttttt aggagtagat tctaataaat cattatttct taatttataa
    35281 tctgtacttt tttctctact gttaatagcg attctaggtt gtgttttttt atcactataa
    35341 gtagcatact ttccaataag atttgagtgt attaatttaa cctttaaaaa atctctaagt
    35401 tctatataca cttcttgttc tttttcaaat ttaatttcaa gtttattggg gtgtatattt
    35461 acatctatac atgatgggtc tacttctata tttaagaagc acactgcatg tttacctatt
    35521 ggaattatag acttataact ttctgttatt gcatctatga ttattttact ttttacaaat
    35581 cttttattta tatagatatg ttgtaagttt ttatttgaac ggtatatatt gttattacct
    35641 atatatccat tcattttaaa gtgattgcac ttaaactcta catctattat attttccgtt
    35701 atttcttttc catatatact tctaatagtg tttacaagtt ttccatcccc tggagtattt
    35761 agcatctgtt tattgttatt tgtatattta aattgaatgt ttggattacc tattgcaagt
    35821 ttatttatca aatcacttat gtttatagtt tctgcatgag tagattttaa aaatttctgt
    35881 cttgctggtg tattaaagaa aatatctttt ataatgatgg tagttccatt agtagaacct
    35941 attggttctt tcgatattat ttttccacct tcaacatata ttttagttcc tattatttca
    36001 tctttagttt tagtcgtcat ttctaatttt gatacagctg aaatagatgc caatgcttct
    36061 cctctaaaac caagagagta taaatcatac aaatcatcaa tttttttaat tttactagta
    36121 gcatgtctca aaaatgattt ttcaacttca cttgaaggta ttcctattcc attatctgta
    36181 atcttaatta gactttttcc tccatcaatt atatctatgg aaattttatt tgcacctgca
    36241 tcaatggagt tttcaataag ttcttttact acagaagaag gtctttctac aacttcacct
    36301 gctgctatct tatttatagt caaatcatct aggatgttaa ttatattttt catttttaca
    36361 cctctaagat tttatttctt ttgcttttct ttgaagatta taaagagagt ttatagcatc
    36421 taatggagtc atatttaaaa tatccagatt taaaatttct tctgatagta tatctctatt
    36481 tacactatca aaagagattt gagttaaggc tacttctttt acagaatcct cttgattaat
    36541 tttctcttta tcatcatttt gcttcattaa agcaacttca tcatttaatg ctttaaattt
    36601 agaatttaat ttatcataat ccttttttat atgtttgtaa tcttttacta taagttcatg
    36661 ttctattttt agagattcat atttattctt aaaattactt tgatttacct taactaattc
    36721 ttcgtccaaa ttactattaa ttatattatt gttttcttta tctccattta ttgcaacact
    36781 gttgtaaaca tgattttttt ccaagtcttt aagtatatat tttgctcttt ctatgacttc
    36841 gtctggtaat ttagctaatt tagcaacata tattccataa ctcttgtctg ctccctgagg
    36901 aattattttt cttaaaaata ttattccttc cccatcttct ttgactgcta ttgaataatt
    36961 tttaacctct ttaaactctt cttctaaatc agtaagttca tgataatgtg ttgcaaataa
    37021 tgtcttacat ctaatatttt tttgaatata ttccactatt gaccatgcta aacttatacc
    37081 atcataggta ctagttccac gacctatttc atctaatata actaaacttc tctctgttgc
    37141 attttttaat ataagggata cttcgttcat ctctaccata aaagtagatt gaccttgaga
    37201 taaatcgtca ctagctccaa ctcttgtaaa tattctatct aatattggaa tatcagcaga
    37261 ctctgctggt acaaatgagc ctatatgtgc cataagagct attatagcag tttgcctcat
    37321 atatgttgat ttacctgaca tattaggacc agttattatg ttaataatgt tttctccacg
    37381 atttaaataa gtatcatttg gaacgaaatt ttcctctcct actatatttt cgacaacagg
    37441 atgtctacca tttcttatat caagtttatt attttcattt attgctggtt ttacgtagtt
    37501 atttatatgt gcaactgttg caagggacac aaaaacatct atattagcta tagtcttagc
    37561 cactttttgt attctatcta tgtttttata tattgtatct cttatctcta caaatatctc
    37621 gtattctaaa gatttaatct tttcctctgc atgtagtatc ttttcttcta tttcctttaa
    37681 ttctggagtt atatatcttt ctgcattact aagtgtttgc tttcttatat atgtctcatc
    37741 tagctttgcc tgtttaaagt tagcttttgt tatttctata taatatccaa aaactttatt
    37801 aaagcctatt tttaatgatt taactcctgt tttttctctt tccctgtttt ctatttcctt
    37861 tacaagaaag gctccattct tagaaatctc tcttagttcc ttaagttcat cactaaaatc
    37921 tgatttaatt atgtttccat cttttatggt tatcgtaggt tcttctagaa tagcttcatc
    37981 tattaaattg tatatatcat ctaatttatc catttctgaa acatactctt ttaggatttt
    38041 tgcatttgat aaatttatag tatcttttaa attaggtaac ttttctatcg aattttttaa
    38101 gtgaattaac tcttttggtg ttactctctc aaatgctatc tttccacata ttctttctat
    38161 atcgtatata tttttaagta tatcatttag gtcttctctt aatataaagt catcttttat
    38221 ttcttctatt acatctaatc tattttcaat tttagattta tttatcaaag gttcttctac
    38281 atattttctt aaaagtctac ctcccattgc tgtagaagtt ttatcaagta cgtgtaataa
    38341 tgaacctttt ttcttattcc ctcttatagt ttgagtaagt tctaaattag ttctagtaaa
    38401 catatctaaa accatatatt ctgatgaatt gtatatattt atattattaa tgttagatgt
    38461 tatttgtttt tgagtattgt atatataatt cagaagaatt gataagcttg actttattag
    38521 acctttatca tcaaacttta gtttttgtag ataaacatct ggaaaatatt cttttaatat
    38581 atttatatcc aaatagttat cactaaaact ttcatttatg tatatattac ttacagtagc
    38641 tatatctcta agcttttcta taaagtctaa atcatttata attatttcag ttggatggat
    38701 ttttgcaatt tcttcaatta ccttatcttc atttaaacat gttgcattag tttctcctgt
    38761 acttatatca acataagtta gacctatatt agtaccatcc ttatacaaag ataataaata
    38821 attattcttt ttattttcaa gtaaatttcc atctaaaact gtaccaggag tgatgaccct
    38881 tataacctct cttctaacaa ttccttttgc tgtagatggg tcttccattt gttcacctat
    38941 tgctacttta tatccatttt caactaattt tgatatatat gaattggctg agtggaaagg
    39001 aacaccacac ataggagcac gttcttctaa accacaagct tttcctgtca atgctatttc
    39061 aagagctttt gaagctacta atgcatcttc aaaaaacatt tcataaaaat ctccaagtct
    39121 aaaaaacagt atacagtctt tatatctatt ttttacttca agatactgtt tcatcatagg
    39181 agttaattta ttcatatcta tttgcattat caaacctcct tcttttctaa ttaaatatta
    39241 tatcatagta gatgattatt attaacacaa tatataaaat tttgacatat aactaaaaac
    39301 aatccaaaat gtatactcac attttggatt gctaagttta atgcttatta aataaaaata
    39361 ttttttttta caataaagat attatttctt ttttaagttt aatgatattt tcaaggttat
    39421 cttcatttaa ctttctacta tctataacaa attctttctt tactattgat ggtttttttg
    39481 aaataacata aattctgttt gatagcatta tagcttcgtc aatatcatga gttataagaa
    39541 gtattgttga atttactttt ttcttaacat ctaaaagcca tttttgcatg gatgttttag
    39601 ttaatgaatc aagtgcccca aaaggctcat ctaaaagcat tatgtcatta gaatttataa
    39661 aagttctcaa gaagttagcc ctctgtctca ttcctccaga aagttctgaa ggatatttat
    39721 cttcatatcc gcttaatcca aacacctcaa aataaggttt taccattgac ctagctttac
    39781 tctttttttc tttttttaaa attagtggta gtgacacatt atctattata gttttatatg
    39841 gcaagagtaa atctttttga tacatataac tatacttacc atttatattc actttgccac
    39901 tatcataatc tgtaagttta gttataatat taaaaatagt actttttcca catccacttg
    39961 gccctaatat agaaacaaac tcaccttcat aaacatcaat acttatatct tttaaaactt
    40021 taatatcttt ataagttttt gatacatttt ctatagatac ttttactttt ttattgtggt
    40081 aaaaattcat ttgtgtaagc atcacttgcc ttcatatcct tatctattaa tttatattct
    40141 tttaagaatg atgtatagtt gttccataca ctatctttca tttctcccca tcgtggtgca
    40201 tcatctttgt acttgcttgc taaatattct tgactcttta aagctagttt ttcatctact
    40261 tcaggtgcat gttttactaa aatttttgct gattcttctg gattttttat tgcatattca
    40321 taaccttttg ttgtagcttt taagaatttt ttagcaagct ctggattatc tttaagtaca
    40381 gtttcattag aaattataag tggtgtataa taatccaatc tctcatctaa atcttttact
    40441 ggtataaagt ttaagtcata tccaattaaa tcagctttaa ctgcatccca accttcaaat
    40501 atccatgcaa aatctacagt tttcatagct gcaaagaaat catcttgacc agtattaact
    40561 atctttaact tattaaaatc agctccattt ttcttcataa ctgctttaaa aacagcttct
    40621 tctgatggag agccccaacc accataagtt tttccttcaa aatctttagc tgttgttata
    40681 ttcttttctt ttggtgatgc aaacccagaa gtattatgtt gtataacagt tgctacagct
    40741 tttattggaa gtggatcttc actagtcttt gcataagtaa cttgttcttg atagcttata
    40801 ccaaagtcag cttttcctgt tgctactaaa gtttctgctc cactttcagg tggttgtact
    40861 atttcaacat ccaaaccttc ttctttatag taacccttat caagtgctac aaataatcca
    40921 gtatgatttg tatttggtgt ccaatcaagc accatagtta cttttttaga agaagtatct
    40981 ttatcattgt ttttgtcttt attgttattt gaagaacatc cagttattgt tgccaatgta
    41041 aaaacactta ctaaacctaa tattcctaac tttttaaaat tcataattgc ctccctcaaa
    41101 tacctttaat ctattaatct atgtcttata acaacttttt ttacagcctg tacaattcct
    41161 acaaacatta aactaaatat aactactaat atagtagatg caaatacttt atcaagtgca
    41221 tatgcacttt ttgacctaag catataaaca ccaagtcctt tcgttccacc tagccattct
    41281 gcaacagttg ctgccattac agcatacgtt gcagatattt taagtccaga aaaaaattta
    41341 tccattgcca taggaaattt aaggtgaatg aatgtattaa ctttatttga gtccatagtc
    41401 ttaaataaat tataataatc tttatcaata ttctcaatgc catctacaaa acttattaat
    41461 ataggaaaaa aacaagtaag cattaccatc aatacttttg gcattgttcc aaatccaaac
    41521 catatcataa agagtggtgc tataattatt gtaggtatca tctgtgtaac caacataata
    41581 ggataaagac atttttttat tattgataca aaatccatca atattccaat taccaatgat
    41641 attataattg cgactataaa accaagtatt gcttctccta tagtaaccac agcatgcatg
    41701 gataaatttt gataatcttt aaaaaacaca ttaattatat ctactggtgt gggtagtata
    41761 tactgaggaa cttctaaaac tcctaccata gattgccaaa gaataagtat aaacaaaaat
    41821 gttgctattg gatatatact atctttgact ttatctatga tttctaagtt tttcatctat
    41881 agtcagacct ccagcttttt catctactct agctactgtt atgactcttt cacatccaag
    41941 attaaaaggg attttatgta ccttttttaa taattcaaaa acttcatcaa aatcaccttc
    42001 aacagtagtg ctcattgctc ctatttcaca tttaagacca cttttttcaa tttcagcaat
    42061 acaagcatct actactttat acatctcttc ttcacctttg taaggtctta gtggcattac
    42121 tgctatgtct gcgattacca tttaaatcac tcctttaaaa ataattaata cctactaggt
    42181 atattttgtt ttttattaaa aaattttatt actaaaaaga tattatattt aaataaaaga
    42241 aaagtcttat ccaaaatagg ataagactta tttttatctt aactggtctc cctacactgg
    42301 cattatccaa acaggttcaa agggtcgaga taaaatctcc tctcagcaca ttagctcccc
    42361 gaaatatatt atatcttttc tacttgatta tactacactt atcatatttt aacaaatttt
    42421 ttatttatat tagatatttt atccgttcaa aaatatctaa tataaattgt acattattac
    42481 accaaataaa ttatcttaaa atgctaattt atcaacatct tccaaaacta gattaactga
    42541 attttctagt ttttattttg ctatttcaag catatttgga tgtatatcaa ttccatacaa
    42601 atattgtgct aaatttctgt tttcactcat ctaatttgca atcgttacag attacagatt
    42661 agaaatagct tttagactct acttatcttt atctacaaaa aatccttcaa taaattcagt
    42721 aataatattc agtaatctaa aaataaacct ctatctagat ttttaagata caagcctttt
    42781 aataagtacc ttttgtgata atataaaaat tactcgtcat tatcatcaaa atcctcagca
    42841 accaacattt tagataagaa tcaaactcta taatataatt attttatcca atttattttt
    42901 gagaaataaa atattttttt aatttttaaa ttttttaata tgcaccttga aaaaagaaac
    42961 gttttcctat attatggtaa tagtagaata aaactaaata ggcaaaacta gagaaattta
    43021 gtgacgcaaa gctataggga ctaagactta tgaaatgagt tatgtcagcc agttgccaaa
    43081 gagatatttt ctttttgttt ttatgaatgt tttttagggg gatatatgat atatgtttat
    43141 ggataaaatg gatagatgta cacatatact aactgcatat atatgcagtt cacgcgatta
    43201 ctgcaatttt atagatacac aattaaatga ttttatacta gaatatggag agaatgtggt
    43261 agaatcttgt ttgcatcaag tgatggtatt ggtaagcaga tataattaaa gaaattttat
    43321 tttctagaaa acccttttta gttttatcta cgttttataa ttcattaaag aactaattaa
    43381 aataagcttc attatatatt tttcatgaag taaacagttg agtttatctc atatactgtt
    43441 taccccaatt acataattct aataatatag attccaatga tttaccacgt ttagtagaca
    43501 aatattcaac ttttggagga atagttggat aagaaatcct cttaataagt caatctcttt
    43561 caaaatactt taattcttgt gacagtatct tatgacttat atgaacaact tcttttttta
    43621 attgactgta acgtattggc tcttgtttta aaatatgcca taaaataatt gccctccatt
    43681 tacttcctat cacagtcatg gtaaaaccaa ttgaacaatt aaaatctttt tgtctttctt
    43741 caatatttgg aaattcttca aaattattat ctatatccat ctcaatccta tctttcaaaa
    43801 tagtaagtat ctaactaaaa agtgcattct tgtttagcgt actaattcgt aatataatct
    43861 tgattgtagt atagagcctt acataatact acttttttag atgtatatgt ttaaatttta
    43921 atatagacat aggttatttt attaatttta tttaaacaga taataataaa taataattta
    43981 tctcaagttt tctatgtaga taaactaatc taatgtttag aaaggattat ataatatgaa
    44041 taaaaaattt cgtctttatc aagttgactc atttacaaag gaaaaattta caggtaatcc
    44101 agctggtgta attacaaatg ctgatggctt atctgaatca caaatgcaaa aaatagcaag
    44161 agaattaaat aattcagaaa ctgcttttat tttcaataac ttggctaaaa atcatcaaga
    44221 aaattatgat gtacatatca gattttttac accaactaat gaggttccta tatgtggtca
    44281 tgctactatt gctgcccatt atgttagagc agtagaaaac caattcaata acaaaacagt
    44341 atttcaaaaa actggtgctg gaatattacc agttgatatt ataaaaaaag ataatgatta
    44401 tgaaataatt atgacacaag gaaaaattga agtacctcca ccattacaac aaacctatca
    44461 aaaaagatta cttaatgcat ttggtatttt aaagaaagat attcgtacag attgtcctat
    44521 tgccatatca tctacaggac attcaaaagt tatgattgga ataaaggaat tatcaacatt
    44581 aaatagttta tctccaaatt tatatgaatt atcgaaaata agtcaagaaa taaaatgtaa
    44641 tggacattat atttttacat tacataataa agaaactcta caagttcatg gaagaatgtt
    44701 tgctcctgca attggtattt cagaagaccc tgttacagga aatgctaatg gacccttagg
    44761 tgcgtacctt gtacattata aagtaatacc tacacccaac acagagttta tatttgaagc
    44821 agttcaagga gaagcaataa aacgcaaagg caagatgtta gtaaaagtaa ttattttaga
    44881 taataagcca gttgaagtaa aaataattgg aaaagctgta attgcattct caacaaacat
    44941 agaaatctaa ttatagattc cttctaagta aatttttgaa ctttttaata ctaatttaat
    45001 aacaaactaa tttatataca atcaa
//
LOCUS       DAENCV010000019        44322 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAENCV010000019 DAENCV010000000
VERSION     DAENCV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.019"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            57..1253
                     /locus_tag="KQD82_000995"
     CDS             57..1253
                     /locus_tag="KQD82_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG4379477.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1346..1870
                     /locus_tag="KQD82_000996"
     CDS             1346..1870
                     /locus_tag="KQD82_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG4379478.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1895..2071
                     /gene="rpmF"
                     /locus_tag="KQD82_000997"
     CDS             1895..2071
                     /gene="rpmF"
                     /locus_tag="KQD82_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG4379479.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2278..2835
                     /gene="fapR"
                     /locus_tag="KQD82_000998"
     CDS             2278..2835
                     /gene="fapR"
                     /locus_tag="KQD82_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG4379480.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2846..3868
                     /gene="plsX"
                     /locus_tag="KQD82_000999"
     CDS             2846..3868
                     /gene="plsX"
                     /locus_tag="KQD82_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG4379481.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3894..4880
                     /locus_tag="KQD82_001000"
     CDS             3894..4880
                     /locus_tag="KQD82_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG4379482.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4989..5918
                     /gene="fabK"
                     /locus_tag="KQD82_001001"
     CDS             4989..5918
                     /gene="fabK"
                     /locus_tag="KQD82_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG4379483.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5934..6884
                     /gene="fabD"
                     /locus_tag="KQD82_001002"
     CDS             5934..6884
                     /gene="fabD"
                     /locus_tag="KQD82_001002"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG4379484.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6884..7633
                     /gene="fabG"
                     /locus_tag="KQD82_001003"
     CDS             6884..7633
                     /gene="fabG"
                     /locus_tag="KQD82_001003"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG4379485.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7687..7911
                     /gene="acpP"
                     /locus_tag="KQD82_001004"
     CDS             7687..7911
                     /gene="acpP"
                     /locus_tag="KQD82_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG4379486.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7940..9178
                     /gene="fabF"
                     /locus_tag="KQD82_001005"
     CDS             7940..9178
                     /gene="fabF"
                     /locus_tag="KQD82_001005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG4379487.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9404..11122
                     /locus_tag="KQD82_001006"
     CDS             9404..11122
                     /locus_tag="KQD82_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4379488.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11153..13153
                     /locus_tag="KQD82_001007"
     CDS             11153..13153
                     /locus_tag="KQD82_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4379489.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKNIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13504..13749
                     /locus_tag="KQD82_001008"
     CDS             13504..13749
                     /locus_tag="KQD82_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG4379490.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13784..14509
                     /locus_tag="KQD82_001009"
     CDS             13784..14509
                     /locus_tag="KQD82_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG4379491.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGMIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14531..15844
                     /locus_tag="KQD82_001010"
     CDS             14531..15844
                     /locus_tag="KQD82_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4379492.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            16050..16589
                     /locus_tag="KQD82_001011"
     CDS             16050..16589
                     /locus_tag="KQD82_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379493.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16678..18663
                     /locus_tag="KQD82_001012"
     CDS             16678..18663
                     /locus_tag="KQD82_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG4379494.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18887..19855
                     /gene="spoIIIAA"
                     /locus_tag="KQD82_001013"
     CDS             18887..19855
                     /gene="spoIIIAA"
                     /locus_tag="KQD82_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG4379495.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGSYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19845..20366
                     /locus_tag="KQD82_001014"
     CDS             19845..20366
                     /locus_tag="KQD82_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG4379496.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20383..20580
                     /gene="spoIIIAC"
                     /locus_tag="KQD82_001015"
     CDS             20383..20580
                     /gene="spoIIIAC"
                     /locus_tag="KQD82_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG4379497.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20596..20982
                     /gene="spoIIIAD"
                     /locus_tag="KQD82_001016"
     CDS             20596..20982
                     /gene="spoIIIAD"
                     /locus_tag="KQD82_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG4379498.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21067..22272
                     /gene="spoIIIAE"
                     /locus_tag="KQD82_001017"
     CDS             21067..22272
                     /gene="spoIIIAE"
                     /locus_tag="KQD82_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG4379499.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22332..22949
                     /locus_tag="KQD82_001018"
     CDS             22332..22949
                     /locus_tag="KQD82_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG4379500.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22961..23515
                     /locus_tag="KQD82_001019"
     CDS             22961..23515
                     /locus_tag="KQD82_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG4379501.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23542..24231
                     /locus_tag="KQD82_001020"
     CDS             23542..24231
                     /locus_tag="KQD82_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG4379502.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24341..24703
                     /locus_tag="KQD82_001021"
     CDS             24341..24703
                     /locus_tag="KQD82_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG4379503.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24788..25294
                     /gene="nusB"
                     /locus_tag="KQD82_001022"
     CDS             24788..25294
                     /gene="nusB"
                     /locus_tag="KQD82_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG4379504.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25278..26348
                     /locus_tag="KQD82_001023"
     CDS             25278..26348
                     /locus_tag="KQD82_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG4379505.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVILNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26345..27550
                     /locus_tag="KQD82_001024"
     CDS             26345..27550
                     /locus_tag="KQD82_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG4379506.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKVKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27552..27767
                     /gene="xseB"
                     /locus_tag="KQD82_001025"
     CDS             27552..27767
                     /gene="xseB"
                     /locus_tag="KQD82_001025"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG4379507.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27770..28657
                     /locus_tag="KQD82_001026"
     CDS             27770..28657
                     /locus_tag="KQD82_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG4379508.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28687..29124
                     /locus_tag="KQD82_001027"
     CDS             28687..29124
                     /locus_tag="KQD82_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG4379509.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29158..31023
                     /locus_tag="KQD82_001028"
     CDS             29158..31023
                     /locus_tag="KQD82_001028"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG4379510.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31156..31968
                     /locus_tag="KQD82_001029"
     CDS             31156..31968
                     /locus_tag="KQD82_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG4379511.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31988..33667
                     /gene="recN"
                     /locus_tag="KQD82_001030"
     CDS             31988..33667
                     /gene="recN"
                     /locus_tag="KQD82_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG4379512.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33882..34748
                     /locus_tag="KQD82_001031"
     CDS             33882..34748
                     /locus_tag="KQD82_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379513.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKYGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34841..35332)
                     /locus_tag="KQD82_001032"
     CDS             complement(34841..35332)
                     /locus_tag="KQD82_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379514.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35393..35833)
                     /locus_tag="KQD82_001033"
     CDS             complement(35393..35833)
                     /locus_tag="KQD82_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379515.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35994..37052
                     /locus_tag="KQD82_001034"
     CDS             35994..37052
                     /locus_tag="KQD82_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG4379516.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFAI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37194..38018
                     /gene="spo0A"
                     /locus_tag="KQD82_001035"
     CDS             37194..38018
                     /gene="spo0A"
                     /locus_tag="KQD82_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG4379517.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38431..39552
                     /locus_tag="KQD82_001036"
     CDS             38431..39552
                     /locus_tag="KQD82_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379518.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39570..40445
                     /locus_tag="KQD82_001037"
     CDS             39570..40445
                     /locus_tag="KQD82_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG4379519.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40469..41161
                     /locus_tag="KQD82_001038"
     CDS             40469..41161
                     /locus_tag="KQD82_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379520.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41169..41993
                     /locus_tag="KQD82_001039"
     CDS             41169..41993
                     /locus_tag="KQD82_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG4379521.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41998..43065
                     /locus_tag="KQD82_001040"
     CDS             41998..43065
                     /locus_tag="KQD82_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG4379522.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43067..43603
                     /locus_tag="KQD82_001041"
     CDS             43067..43603
                     /locus_tag="KQD82_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG4379523.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43671..44276
                     /locus_tag="KQD82_001042"
     CDS             43671..44276
                     /locus_tag="KQD82_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG4379524.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVIGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
BASE COUNT        17950 a         4472 c         8304 g        13596 t
ORIGIN      
        1 tacaaatcaa aatttattac atattattta taaaaaaatc taaggaggaa ataataatga
       61 aaatattagt attaaactgt gggagttctt cattaaaata tcaattaata gatatgaata
      121 atgaagaagt tttatgtata ggattggttg aaagaatagg aatagaaggt tctatattaa
      181 aacatgaaaa agcaggtaga gatgataaat acgttgttga acaaccaatg aaggaccaca
      241 aagatgcaat agcattagtt ttagaagctg ttgctcatcc agaatttggt gcagttaaag
      301 aaatgaaaga aatagatgca gtaggacata gagttgtaca tgctggagaa aaatttgcaa
      361 cttcagtagt aataactcca gaagtagaag aagctttaaa agaatgtata gatttagctc
      421 cacttcataa tccagcaaac ataatgggaa tagatgcttg taaagctata cttccagatg
      481 taccaatggt aggtgtattt gatactgctt tccatcaaac aatgcctaaa tcttcatact
      541 tatatggttt acctcatgag ctatatacta aatatggggt aagaagatat ggattccacg
      601 gaacttctca taattatgta tctcaaagag cagcagagat actaggaaaa gatataaaag
      661 atttaaaaat agtaacttgt cacttaggaa atggtgcttc tatagctgct gttgatggtg
      721 gaaaatgtgt agatacatct atgggattca ctccattaga aggattaata atgggaacta
      781 gatgtggaga tatagaccca gctatattac cattcttaat gagaaaagaa ggtttagatg
      841 ctgatggatt ggataaatta atgaataaag aatctggagt atatggaatg actggaattt
      901 ctagtgactt cagagatata gaagatgcag ctaaaaatgg agacgaaaga gctcaagcta
      961 cattagaagc gtatgttaaa aaagtacaaa aatatatagg cgcttatgct gctgaaatga
     1021 atggattgga tgttgtagta tttactgcag gcgttggtga aaatggaaag gctataagag
     1081 cagatattgc ttctaacatg gagtttttag gaatgaaatt agataaagaa gctaatgatg
     1141 ttagaggaaa agaaacagta atatctactg ctgattctaa agtaaaaatg cttttaatac
     1201 caactaatga agaattaatg atagctagag atacattaag attggtaaaa taattaaatt
     1261 aaacctatgc ctatcaagta aaaatacttg acaaacaaat gctagatttg tataataaat
     1321 ttggtgatat gttgaggtga acttaatgaa agttagcata gaaaaaataa atagaaaaga
     1381 aactgacaaa atagacttga atttttgtga aaaaattgat actattagtt attgtgatga
     1441 gatttacaaa ttagtatcac ctgtaaattt aaaagggaaa gtgtcaaaga ctaataaagg
     1501 tttgtatcta gatatagatg tcaattttac aattgttgac aattgttcaa gatgtcttaa
     1561 agaagtagaa ataccactag agtattctat acaaggtttt ttagtaaaag aagaagatta
     1621 tgatgaagat gagtttgagg aatttgatcc ttttatattc gatggtgaag aaatcgacct
     1681 tattgatata atagaacaga cattagattt taatgtacct cataaagttc tttgtagtga
     1741 aaactgtaaa ggtctttgtc aagtatgcgg ggcaaactta aatgaagaag agtgttcttg
     1801 cagtgaaatt acaaacgatg aggaatacat agatcctcgt tttgctaaat taaaagattt
     1861 atttaattaa attttgacta aggaggtggc gtaaatggca gtaccaaagc gtaaaacgtc
     1921 taaatcaaac acaaaaatga gaagagcagc taactcaaag atggaagcaa caggatttgt
     1981 aagttgccca caatgtcatg agccaaaatt accacataga gtgtgtccag actgtggata
     2041 ttataaaggt aaagaagttg tgtctaagta atagcttcta aaaagcatgt agagtatact
     2101 ctacatgttt tttttgtaca tataaaaaaa taagatgatt tactagagaa aataagtgga
     2161 ttttatgcat attaaaaaat agaaaaataa taatataagt attgaaaatt tttgtgtgtt
     2221 actatatact actattagta gctggtacta attacagcta taaaatttgg agggaacatg
     2281 aaaaagaaaa gtaaagccca aagacaaaaa gagcttatag atatgctaaa gacagatcct
     2341 ttttatactg atgaagaatt atcaagtctg tttgatgtaa gtatacagac cataagatta
     2401 gatagaatga gtctaaacat accagagcta agagaaagag tcaaatcaat agcagaaact
     2461 cagagttcaa aagtaaagac tttaggagta aaggaaatta caggagaaat cattgattta
     2521 tctgtagggc gattaggtat atctatgtta gaagttacac aggacatgat ttactctaag
     2581 actaatactt taaaagatac gtatattttt tctctagcag attcattggc aatggctata
     2641 atagatgcac ctaaggttat tatgagagtt gcaaatgtaa aaagttttaa gttgatagaa
     2701 caacaagata gacttatagc aaaagcagaa gtgtatagaa atatagataa aaagcattat
     2761 gtaaaagttg ttatcaacaa taaagcccaa gagcaaatat ttagaggaaa gtttatcttt
     2821 gaagaattag attaggaggg aaattatgaa aatagtaatt gatggaatgg gtggagataa
     2881 tgcacctaaa tctaatgtag aaggcgcagt aaatgctata aaagaatatc aggttgattt
     2941 aataatcaca ggagataaag atttacttga aaaggaattt tcaaattatg aatttgatag
     3001 aaataaatta gagatagttc atactactga gattatagaa aacgaagata aaccagtaaa
     3061 ggctataaga agtaaaaaag attcatctat ggtagtagca ttaaatcttg taaaagaagg
     3121 aaaggcagat gctataatat cagctggaaa tacaggagcc ttacttgctg gtggattatt
     3181 tgttgttggt agaataaaag gaatcgatag accttgctta tgttcagcaa taccaaatgt
     3241 aaagcgagga atgacactta tagcagactg tggcgcaaat gcagattgta aacctaagaa
     3301 tctagttgaa tttgcagcta tgagtaatat ttattcaaga aaagttttag gtcttgaaaa
     3361 tcctaaggta gcattagcaa atgttggact agaagaaggc aaaggcaatg atttagtcaa
     3421 aaggtcatat gaagaaatta aaaaattaga tttaaatttt atagggaatg tagaagctag
     3481 agaagttata aatgcataca cagatataat catatgtgat ggatttacag gtaatatatt
     3541 acttaaatct gctgaaggtg tagcactttc agttatgagt cttataaagg aaacttttat
     3601 ggccagtaca aaaagtaaaa taggagcatt acttataaag gatgatttaa gaaaattaaa
     3661 aagttttata gattattctg agtatggggg agctccactt ttaggtctaa atggaggagt
     3721 tataaaagct catggtagtt ctgatgctaa agctataaaa aatgctataa atcaaggaat
     3781 aaaattttca aagggaaaag tcgttgaaga tattaaccaa tttataagca aatacaatga
     3841 agaaaataaa aataacgaag atgaataaac tcgttttgga ggtttaatat ataatgaata
     3901 caaaggctgg aatattagga gtaggaagtt atctacctga gcaatcatat gataattttc
     3961 acttcgaaaa aataatggat acatcagatg agtggataag tactagaact ggtattaagg
     4021 aaagaagatt tgccaaagag tctgaagcaa cttctgactt agcatctaaa gctgccttaa
     4081 aagctataga atgtgcaaag ttaaacgtag aggatataga gctgattata ttagctacaa
     4141 ttactcctga tatgtctttg ccttcaactg catgtatagt acaagatgca ataggagcag
     4201 tgaatgctac tgcatttgat atatcagcag cttgttctgg atttgtatat ggagtaacta
     4261 ttgcaaagca atttgtggaa acaggttgct ataaaaatgt gcttgtaata ggagcagaaa
     4321 cttgttctaa gtttttaaat tatgatgata gaacaacagc agtattgttt ggagatggag
     4381 ctggagcagc tgttattggc cctgtaaatg agggtggaat actgtctaca catatgggct
     4441 ctgatggtaa aggaaaagat tgtttaaaag tacctgctgg aggttcaaga cttaaggcaa
     4501 gtaaagagac tgtagaagct aatctacata caatagaaat ggcaggtagt gatgtgttta
     4561 aatttgcagt tagaaagatg gcagaaactt ctttaagagc tcttgaaaaa gcaaatctaa
     4621 atacaactga tatagactac ttagtgccac atcaagctaa tataagaatc atacaagctt
     4681 cttctaagag attagagctt gatatgaaaa aagtttatgt aaatatagac aaatatggaa
     4741 atatgtctgc tgcatctata ccagtagcat tagatgaagc ttatagagaa ggtaaaatta
     4801 aaaagggtga taatgtagtt ttagttggct ttggtggagg tctaacttgg ggggcttcag
     4861 ttgtcaagtg gactttatag tataaaatat ctaaaatgat ttaattaaca aaattaaact
     4921 tatacacaaa ataaagttga ttaataataa aaagtttact tttatgattt caaatgaagg
     4981 agaattgtat gaataaaatt tgcaaaatat taaatataaa atacccagtt atccaagggg
     5041 gaatggcatg ggtagctact gcatcattag caagtgctgt atctaatgca ggaggacttg
     5101 gcataatagc agcaggaaac gcaccaaaag aagctataaa gaaagaaatt gttgagtgta
     5161 aaaaattaac agataaacct tttggagtaa atgtaatgct tatgtcgcca tttgttgatg
     5221 atataattga tttgattata gaagaaaaag ttcaagttat tactactggt gctggaaatc
     5281 ctgcaaagta tatggataga ttaaaggaag ctggaacaaa ggttattcct gtagtaccta
     5341 caatagcttt ggcacaaaga atggaaaagc taggagctac agcagtaata gcagaaggta
     5401 ctgaaggtgg aggacatata ggagaactta ctactatggt cttagttcca caagttgctg
     5461 atgctgtaaa catacctgta atagctgctg gaggaattgt agatggtaga ggaattgcag
     5521 catcatttgc attaggtgcc agtgcagttc aagtaggaac tagatttatt tgcagtgaag
     5581 agtgttctgt ccattcaaac tataaaaact tagtactaaa agcaaaagat agagatgcaa
     5641 ttgtaacagg aagaagtact ggtcatccag taagaacatt aaaaaataaa ctatcaaaag
     5701 aatttttaaa gatggaacaa aatggagcta ctcctgaaga actggataaa aaaggtacag
     5761 gagctttaag atttgcaaca gtagatggag acatagaaaa aggttcattt atggcaggtc
     5821 aaagtgctgc tatggtaaaa gaaataacac cttgtaagga aattatagag gctatggtaa
     5881 atcaagcaag agagattatg ccagcaatag aactgtaaat caggaggagc aagatgggaa
     5941 aagttgcact agtattccct ggtcaaggag ctcaatatgt aggtatggcc aaggatttat
     6001 atgaaaataa tgctgttgct aaaagtgtaa tagatgaggc tagtgatgcc ttaaatatgg
     6061 atttaaaaaa ccttatgttt aatggaaatg aagaagaact ttctaaaact gaaaacactc
     6121 aacctgccat ggttacacat agtgtagcag tattaaaagc ggtacaagct caaatagatt
     6181 taaaatataa tgcatgctta ggattatcct taggagagta tagtgcgttg gttgcagcag
     6241 atgcaattga ctttaaagat gctgtatgtt tagttaaaaa aagaggtaag tttatgcagg
     6301 aaacagttcc agcaggtgtt ggagctatgg cagcagtttt aggtttagat agaaatgtac
     6361 ttgaaaatgt ggttaaagat atacaaggag gtattgtaga agtagcaaat tacaattctc
     6421 ctggacaaat agttatatct ggtgaaaatg aaaagataga agaagcaatg gtaaaatgca
     6481 aagaagcagg agcaaaaagg gcagttaaat taaatgtaag tggacctttt cactcatcaa
     6541 tgttaaaaga agctggtgta aaattggcca gtgaattaga aaaagttaac ataactaaac
     6601 ctaaagttga tgtggtggca aatgtaaatg cagattacta taaaaacgaa ggtaaagacc
     6661 tgcttataaa acaagtaagt tcatctgttc tttgggaaga ttctatagaa agacttttaa
     6721 atgatggata tgatactttt atagaaatgg gacctggaaa aactttaaaa gcatttatta
     6781 agaagatagc ttccaataaa aaagcaaatg tcaatattta taatatagat ggtatagatt
     6841 ctcttaatga atttgtacaa aattatagaa atggagagat ataatgataa atcttacagg
     6901 acaagtagca gttgttactg gtggctctag gggtatagga aaagaaatag caaaaaaact
     6961 agcatctttt ggagctgatg tagtaatcaa ttatacttct aaagaagatg aagcactaaa
     7021 aactaaaaat gaaatagaaa gtatgggggt aaagtgtacc tctataaaat gtgatgtatc
     7081 taaatttgat gaagtgaatc aaatgataga ttctgttgta agcgaatttg gaaaaattga
     7141 tatattggtt aataatgcag gcataactaa agatggtctg cttatgagaa tgaaagaaga
     7201 agattttgat agagttatag atataaactt aaaaggtgtc tttaattgta caaaagcagt
     7261 tactaaacct atgatgaaaa agaagtatgg aagaataata aatatgactt cagtagttgg
     7321 aattatgggt aatgcagggc aaactaatta ttgtgcatca aaagcaggtg taattggatt
     7381 tacaaaagct tctgcaagag agttagcatc aagaaacata aatataaatg cagtagcacc
     7441 tggatttata gaaacagata tgactaaagt actaagtgat gatgtaaaag aatcaacact
     7501 agcaaacata ccaaagaaat cttatggtaa accagaagat gtagccaatg ccgtagcatt
     7561 tttagttagt gacatgtcaa gttatataac aggacaagta ataaatgtag atggtggaat
     7621 ggtaatgcaa taataattta aaaagtagag aaatctatta aaataaaaaa ttttaggagg
     7681 tacacaatgt ttaataaagt tgtagaaata ataatagagc aattaggagt agaagataaa
     7741 gaaataacta tggaaacatc attaatgaag gatttagaag cagattcatt agatgcagta
     7801 gaaataataa tggcacttga agatgaattt ggtatagaaa taccagatac agaagcagaa
     7861 aacttcaaat ctataggaga tatagttaag tatatagaag ctaataaata gtcgagcttt
     7921 aaaaaagtgg aggtatccga tgaataaaag agtagtaata acaggacttg gatgtgttac
     7981 gccgttggga acaggaaaag aagagttctg gagtaatata aaatctggag tatctggtat
     8041 agataaaata actaattttg atgctagcac atatcaaact cagatagcag gagaagttaa
     8101 gaattttcat ccagaagaat atataagtaa aaaagaattg aagagattag ataaatttgc
     8161 tcaatttgca atagtatctg ctaaattagc agttgaagat gcaaatcttg atttagataa
     8221 agttgataga gaacgttttg gtgttataat aggttctgga ataggtggag ttgaagccat
     8281 agaaactcaa cataaaattc ttttggaaaa aggaaataaa agagtaagtt cactttttgt
     8341 acctatgatg ataggaaata tggcagctgg tcaagtttca atatttttag gagcaaaagg
     8401 gcctaacact aatgtatgta cagcttgtgc atcaggaact cattcaatag gtgatgcttt
     8461 taaggtaatt caaagaggtg atgcagacat tatggtagca gggggttctg aagctgctgt
     8521 aacaggtctt gcttttgctg gattctgtaa catgaaggct atgtcaacta gaaatgatga
     8581 ccctaaaact gcatcaagac catttgataa agacagagat ggttttgtaa tgggagaagg
     8641 agcaggtatt gttatcttag aagacttaga acatgcttta gcaagaggcg ctaaaatata
     8701 tgcagaagta gttggatatg gtttaactgc tgatgcatac catatgacaa ctccagctga
     8761 aaatggagag ggagcagcta ggtctatgaa tatggctttg aaagatggaa atgttccatt
     8821 agaagaagta gattatataa atgctcatgg aacttcaact tattacaatg atttatatga
     8881 aactatggct ataaaaactg tatttggaga aaaggcatat gacttatgtg tgtcatcaac
     8941 aaaatctatg actggtcact tattaggagc atcaggtgct atagaggctg ttgtttgtgc
     9001 tatgagtata gaagatagtt ttgtgcctcc aactataaac atacaagaag ttggtgaaga
     9061 tttggattta gattatgttc caaatcaagg taaagaaaaa aatattagat atgcactatc
     9121 aaattcttta ggatttggtg ggcataatgc tacaatagta ctaaaaaaat atgtataaat
     9181 aaatattaaa tataaaacta aaaaacaagc acttgtttta actgtataaa tttatattag
     9241 cttaaattta aagtctccta aattatttta ggagactttt ttattaagat ttaagtctag
     9301 gatatgttgt aaatcatttt gatatattgt attattaatt attaagaggt tatttatata
     9361 tgcaagtttt cattctacaa tatacaaaag gaggactaaa attttgaatt ttagactacc
     9421 aaagaaaagg ttacccaaaa agataatttt tatatgtata atacttatta gtattatttt
     9481 gtttataaag tatgaccaaa atggattgaa agaaagagaa attataaata agggaaaata
     9541 tatgtctgaa agccttgtgg cagataagga gtatgatgaa gcaaaaaaag tggttaagac
     9601 ttcttttgaa tctattccag aaaaaaaata caataaagta tataatgaag tttgggatat
     9661 gttaaagact atttcatatg ttccagatgg aacgaagata gtcattgatt ctttagaaaa
     9721 gatgagagaa tcagatgtga aattatctga tgaatgtaga tttaatatag aaaaaagatt
     9781 agcatcagtg tatataatgg ataataatta ttctaaggca gtagatttaa ctgtaaagtc
     9841 tataaagttg gctgagaaat tggggaataa ttatgaaaaa gcaagactag atgttgattt
     9901 ggcgagcata tttttaaaga taggtggcta tgaaacagga gagaaattaa taaaggattc
     9961 ttttaaaata gatattgatg aaggtgaaaa aaatggaatg gttagattat atgctattat
    10021 aaatctagca gaaatatatg tagaagaggg agaatatgat aaagctattg aaataagtag
    10081 caggataaaa gattataaaa agtttgtaaa tacggataat tacaatgatt ttgatataat
    10141 ggcctctata atgcagtcaa atgcgtatgt gggcaaaaat aatatacaaa aggcaaagac
    10201 ttttttagaa aaagctgatt atcttataaa atcagataga acagtttata tattagataa
    10261 agatatgtac tattatatgg caatgggaaa cttagaatat aaaagcgaaa attataattc
    10321 tgcaatagat aattataaaa aaagtttaga gatatctacc aaaagaaagt taacccagga
    10381 gaaaataaaa aatttaaata agatattaaa tatatatgaa aaacaaggaa atatagaatc
    10441 tttaaatgag tatcaaaaga tgttaataaa tgaatataaa gagaacaaaa gtataagaga
    10501 ttcagagact agtttctata taattgataa agtatcaaac gaaagtaagc tatttgaaaa
    10561 aactaaaaaa gaaataaaat attataaaat attatttacc gtaattttaa ttgctatatt
    10621 agggtctact tttttataca accgattgaa gtattttaaa gcacaaaatt tacatgatgg
    10681 tcttacaaat gtttataata gaaaaagttt tgatattatg tatgaaaagt acagagagaa
    10741 agatgataat tttgcacttg tgatgataga tattgataat ttcaagctca taaatgataa
    10801 ttatggacat aaatttggtg acactgttat aaagggaata acaaatacca tttgtgttat
    10861 gttagaacaa gatgataaag tctttaggta cggaggagaa gaattttctg tattaataag
    10921 aaataaatct ggtgaggaag tagcagatat aattgacagt ataagggttg caattgcaaa
    10981 taaaaagtgg aatgatgatg tgactgtgac tataagtgca ggtgtagctc atatttcaga
    11041 tagcaaagat gtactagaag aagctgataa aaatttatat aaagcaaaac aacttggtag
    11101 aaataaagta gtatataagt aattatatac tatataaata agaggtataa atatgaaaca
    11161 atcaatagct atagtaactg ataaaataac taaactatca tctttcttag aagaaaatat
    11221 acgtttggtt ttaggtaatt atattgatat aaaccactat tatctggaag ttttaaaaga
    11281 gaatgataaa atacaaggtg attttgtact agtaatgaat gacgatagat taaacagtat
    11341 gagaaagcat ttagtacaag atagtaagat tatagtgata aggagaacac ttaaagaaaa
    11401 tgaaatatat ggattatttt caataccaaa tggaactgaa gtattggttg taaatgatac
    11461 aaaagaaact accttagaaa cgataagctt attttacaaa ataggagtaa aaaatttaaa
    11521 gtttactcca tatattgaag gaaaaattta taaaaatata gacatagcta taacgcctgg
    11581 agtaaaggga aaagtaccaa gttatataaa taaagtaatc gaccttggaa atagatatat
    11641 tgatatatct acctttatag aaataataaa tggtcttaga atagatgtta aagaaatacg
    11701 taaaaattta atggcatatt ctgagagttt aataagttta gataatggag ttaaagataa
    11761 ttataggcaa ctgtttttaa aaatagaaga acaagatgtt atattgaatc tatcaaaaga
    11821 tggaataata tttacttcag cagatggtat aataaacaca tttaataaag aagctttaaa
    11881 aattttaggt ataaatgaaa atccacaagg caaaaacata gaagatatca tttctgaaga
    11941 tttaaatatt ttacttgaag acaaagaaat aattgatgaa gtggtattta caaataaaaa
    12001 atatataaat gtaaataaaa aaaatatttt ttctatgggg aataaagctg ggatatatta
    12061 taacttacaa gaaataacgt atataaaaaa attagaacaa aatcttacta ataaattaag
    12121 agaacaaggt cagatagcaa gatatacatt taaagatata aaaactaaaa ataaaaatat
    12181 ggtaaagtgt gtagaattgg ctaaaaaaat atctaaatca gacctttctg tattaataat
    12241 aggagaaagt ggtactggta aagaattatt atcacaatca attcataatg cttccaatag
    12301 aagtaatcaa ccgtttatag cagttaattg tgcagcagtt ccagatagtc tattagaaag
    12361 tcaactgttt ggatatgaaa aaggctcttt tacaggagct ttaagagaag gaaaaaaggg
    12421 tctatttgaa cttgctaata atggaactat atttctggat gaaattggag atatgcctcc
    12481 attgctacag acaaagttac taagggtatt acaagaaaag caggttatgc ctataggttc
    12541 ccataatgtt ataaacatag atgttagggt catagcagca acaaacaaaa acttactaaa
    12601 gatgattaga gaaggaaaat ttagagagga cctgtattat agactaaatg tattgccaat
    12661 agatgtacca tcattgagga atagaaaaga agatataata acacttatga atttcttttt
    12721 gaaaaacaat ataagacttt cgccagaagt tataactatt ttagaaaaat ataattggcc
    12781 aggaaatata agagagttac aaaatgttgc ttcttatgta agtctaatgt gtgatagtat
    12841 ggtttttaga gatgacttac caccatatat aagggtagaa tgtgctaaca attatgaaga
    12901 tggatttggt tatacaagaa aagaatttga ggaaatactg aggattctat atgaaagaag
    12961 agagtcatcc tctggaattg gaagagggtt tataatcgaa aagcttatac aggaaggtat
    13021 tgagatttct gaaagcaaag ttagaaaggt gctatcttat ttaagtgaat gtggatacat
    13081 atcatctaaa tctggtagag gaggaagtca aataacctca gaggggataa aactttataa
    13141 taatataaat taagctaagt atttgatatt gaaatcacat attttgaatt tagatattta
    13201 aataataata aacttttata tttatagaga ttaatttata ttatgtcaga aagaatattg
    13261 taatttacac ttaaatatta ataggttttt aaaataggat tagataggta ataggttaag
    13321 ttagaataac ccattaccta tttttatttg taagaaaagt gtacatagaa ttagaagtaa
    13381 aaaatacaac taataaaact attgaaaata ctagataaat aataaataaa aataattata
    13441 gattttggca tacatattgc taattataga gataagacga caaaataatt gggaggttta
    13501 atgatgaaat acaaaacaac agggacttgt gctacagaaa ttgaatttga agtaaaagaa
    13561 aataaagtta caaatgtaaa ctttataggt ggatgtgatg gaaatttaaa agggctaaaa
    13621 gtattagttg aaggcatgaa tatagaagat gtaatacaaa agttaaaagg aatagagtgt
    13681 aaaacaaagc caacatcttg cccagaccaa ctgtctctag cattggaaaa ttatatgaat
    13741 ataaagtaat acataaaaca atcgattagt taggagttta attatgaaac caataatagg
    13801 gattttagga aatttaataa taatggaaaa tggtatgttt ccaggtcttg aaaggtcata
    13861 tgtaaataat gattatataa atgcagtgct aaaaggtggc ggtagtccag taataattcc
    13921 tgtaaataca gataaagaag ttataaaaaa acagattgaa atggttgatg gagtcttaat
    13981 atctggggga tgggatatca atcctcaact gtatggagaa gaaacaagag aagaaacgac
    14041 atttatatat ccagaagtgg atgaatttga tttaattgca ataagtatag cattagaact
    14101 taaaaagcct atattaggtg tatgtagggg actacaaatt ttaaatgtaa gtttaggagg
    14161 aactttatat caagataata atcttataga aggaagttat ataaaacata cacaatcttc
    14221 taaaagacat gtagctactc ataaagttga tgttaaagaa ggaagtattt tagagggaat
    14281 tttaggaaaa caattactta caaatagtta tcatcatcaa agtgtcaatc agcttggaaa
    14341 aggattaaaa gccatagcat actcaaaaga tggtatgata gaagctattg agaaagagga
    14401 tgaaaatttt gtagtaggag tacagtggca tccagaaatg atggtagatt actgtgataa
    14461 aatggaaaaa ctatttaaat attttataaa tatttgttcg aatttataat ataccttggg
    14521 gggattgaac atgaacgaaa agaacaaaat gggattaata agtattatac ttttaggtat
    14581 taatgcagta gtaggagctg gagtttttct attaccagga gatgcaatga aatcatttgg
    14641 tgtagcaagt atatttgtat atatatttga tatgttgtta gttttatcta tggcattctg
    14701 ttttgctgag gttgcaggta aattcaataa aaatggtgct gcctatgtat atacaaaaga
    14761 agcttttgga gatttttgtg gttttgaagt aggtcttatg aaatgggtaa taggatgtat
    14821 ttcttgggga gctttaatag taggttttcc tacatcatta tcagcagtat gggctccagc
    14881 aggtgaacca catatacaaa aaattataat tgtagccatg atagttgggc ttacaattat
    14941 aaacttatta ggtgtatctc tatcaaaaat tgtacaaaat gttataactg taggtaaact
    15001 aattccatta atactattta taggaatagg tatatttttt ataaaaggag ttaattttac
    15061 ttcttcaaca atggtacctc caggagctgg agcaacagag tttggagcag cagcactttt
    15121 aatgttttat tcatttacag gatttgaatc tatagcagtt gcagcagaag atatggagaa
    15181 tccacaaaaa aatataccaa tagcaattat aagtgtaata gtaattgcat caataatata
    15241 tatattaaat caagttgtat gtgttggtat actaggagat tcattatcta gtacatctac
    15301 accagtggca gatgctgcaa gaatatgctt tggaaatatg ggagctggat tggttacctt
    15361 tggaacattg gtttctgttg gtggaatttg tatgtgtggt gcttttgtaa atccaagaag
    15421 ttgtgtagct ttagctgatg ataaaatgtt accgagaata tttgcaagaa aagataaaaa
    15481 aggtacacca tatgtagcga taatagctac tatgttaatc actataccaa tagctctatc
    15541 tggaagtttt gctgaattag cagcaataag tgcagttgct agattcatac aatacatacc
    15601 aacatcttta tccgtattag tgtttagaaa gaaaagacca gaattagtag gtacttttaa
    15661 aactcctttt ggagcagtga ttcctctaat tgctgtatgt gttggaacat ggttattatt
    15721 ccaagcatct atgcatcaat taattatggg attaggagct ttggcaattg gagtaccatt
    15781 atactttata atgaagtctt ataatagaaa agtctatggt gaaaatttaa agaaaatagt
    15841 ttaattatgt tataacagtt tattgagtta aagttagaca ttggcagaaa attaaataca
    15901 aataagtatc tataaggcta tattaaaata ttattaattt tttattttaa tatagcctta
    15961 aattgttatc aataagtgaa aaatattgta acaatagaag aacaatagta ttatgtttat
    16021 agacaaaaaa cttattagga ggtaaattta tgccaacaat aacattaaaa aatggtgtag
    16081 acgtacttat tagagaagga gtaagggaag atgctcaaag cattatagat ttttataatg
    16141 aagtgggtgg agaaactcat tttctgtcat ttggaaagga tgaatataaa atatctttgg
    16201 aggaacaaga aaatgctata gaatcagcaa aagcatctga taattctgtt aaactgattg
    16261 catttataga tggtgaaata gttgggattg caacgattga ttctaatcaa aaagcaaaag
    16321 gaaagcatgt tggagtatta ggaattgttg taaaagaaaa atattgggga attggattgg
    16381 gtaaaaggct tatgttagac cttatagaat ggtgtaaatc aaatggaata accaagaaga
    16441 taactcttgt cactaatgaa gaaaattata atgctatagg attatacaaa aaagttggat
    16501 ttgaagtaga aagcatacta aagaaagaat gttattataa tggtgtttat actgatttaa
    16561 taggaatgtc acttttattg ggaatataat tagtgtacat tttatctcga aaaaagtata
    16621 ttaagtatgg tataattaaa aaagaatata acttaatata attttggagg acgaattatg
    16681 aaaaatctgt gtaaaatatc agatgattat ttaaacagca agcttaagta tttaaagtta
    16741 ttatcaaaac aatatccaag tatatcaaaa gcaagtacag aaataataaa tttagaggct
    16801 atattaaatc ttccaaaggg aacagagcat tttataacag atgtacatgg tgaatatgaa
    16861 ccatttgtac atgtattaaa aaatggttct ggtgtaataa agagaaaaat agaagaattg
    16921 ttttcaaata caataagaga cagcgaaaaa aaaatgttag caactcttgt atattatcct
    16981 gaacaaaagt tagacctaat aattaagcaa gaggaaaata tagatgattt ttatagaata
    17041 aatatttata gacttataga actttgtaaa tatgcttcta gcaaatatac taggtctaaa
    17101 gttagaaaac tgttaccaga aaattttaaa tacataatag aagaactttt gcatgagcat
    17161 gttaaaagtg aacataaaga agagtactat aaaagtatag ttgaaacaat agttgacata
    17221 ggaatagcta aggagtttat aattgctatt tctacagtta tacaaaaatt ggttgtagat
    17281 agacttcatg taataggaga tatttatgat agaggtccaa gaccagatat aattgtcgat
    17341 aagcttattg aacatcattg tgtagatatt caatggggaa atcatgatat attgtggatg
    17401 ggtgcagcct caggtgaaaa aacttgtata gcaaatgcac tgaggatatc agctagatat
    17461 gcgaatttag acattgtaga agacatatat ggaataaatc tgttgccttt ggccactttt
    17521 gctatagaaa tgtataaaga tgacccttgt aaagaattta ttccaaaggt taatgaccaa
    17581 agtgtaacaa caacagaaaa gtctttaatg gcaaaaatgc acaaagcaat aagtataatt
    17641 caatttaagc ttgaaggtga ggttataaga cgaagacctg aatttgaaat ggaacataga
    17701 ctcttgctta atatgataaa ctatgatgaa ggtacgatta atttaaaagg taaaacatat
    17761 aaattaaaag atacttattt acctacaata gacaaaaaag acccatataa attaactatg
    17821 gaagaaagaa atgttattga taaattagta tcttcattta gaggtagtga aaaattacaa
    17881 aaacatgttt catttttgtt ttctaaagga agtatatatt taaaggctaa ctcaaattta
    17941 ctgattcatg gatgtgtacc gttaaatgaa gatggaagct ttatgtctat gaatataatg
    18001 ggaaaagagt ataaaggtaa ggctcttatg gataagatgg agtctctagc aagagaagga
    18061 tttttcttta aagataaggc agaagaaaaa ctgtatggca tggatattat gtggtattta
    18121 tggactggga aatgttcatc attatttggt aaagatgata tgactacatt tgaaagatac
    18181 tttatagctg aaaaagagac tcataaagaa aataagaacc catattttaa acttagagaa
    18241 aatgagatgg cttgtaaaag attatttgaa gaatttgatt tagaacttga tgaatctcat
    18301 ataataaatg gtcatgttcc tgtggagagt aaaaatggag agagcccaat aaaagctaat
    18361 ggtaaaattc tcgttataga tggtggtttt tctagggctt accaaaaaac tactggtata
    18421 gctggataca ctttgatata caattctaga actttgcaat tagtatcgca tgaaccattt
    18481 aattctgctg aagaagccat tgcaaatgaa agtgatatct tatcgacaac tgttgtagta
    18541 gagcataagg caaagagaaa aatggtgaga gatactgatg aaggagtaaa gatacaagag
    18601 gaaatagaag atttaaaact acttttaatg gcgtataaga aagggctaat aaaagaaatg
    18661 tagatgttat agaggcactt catgattgaa gtgtcttttt gttaatactt attaaatata
    18721 ttataaagat taataatagt agtttatatt atacttacta agtgaatcca agataaatgg
    18781 gttcactttt tttatttata aacattatta aaaaaatata gatgttttgg ttatattcct
    18841 tgtcctttat aaatagaatt atataaacaa aggaggagga caagagatga ataaactttc
    18901 tgatgaaata ataaactctc tttctacaac tataagagaa aaggtagaaa aagtctcaaa
    18961 caataatctg aacatagaag aaattaggtt acgctcacaa aaaccattga tactaaatgc
    19021 aaactctaaa gattattttt acaatcaaaa aactatgaca cttgatttaa atcaacaaaa
    19081 ctcatatgta gtaacaagag aagatgtaga gcaaactttt caaataatat gtaaatattc
    19141 aatacattca tttatggatg acataaaaaa aggattcata actttgagag gaggtcatag
    19201 agtagggtta gttggaaaag caatagtaga agatggacaa gtcaaaaata taaagcatat
    19261 atcatcttta aatataaggg tatcaaggga aattattgga tgctcggata agattttaag
    19321 tcatatcata aagggaaaaa atcagattaa taatactttg ataatatcac cacctcaatg
    19381 tggaaaaaca actttaataa gagatatagt aagaaattta agtaatggta atgaagatta
    19441 tggatttaaa ggtttaaaag tggctttagt agatgagcgt aatgagatag caggatctta
    19501 tctaggtgtt ccacaaatgg atgtgggaat aaggactgat ataatagaaa cttgcccaaa
    19561 agacttgggt attacaatgc ttttaaggtc tatgtcacca aatgttattg tgacagatga
    19621 aattggaagt gaaaaagaaa taaaggcatt atacacagca ttaaatggag gtataggatt
    19681 aataactact gtgcatggag attcaataga ggatatacaa aatagaaaag aattaaatag
    19741 acttctggat aaagagttat ttaaaaaggt tataattctt tctgcaaaaa gaggagcagg
    19801 aacaattgaa aaaatttatg atttagaaga aaagagatgg tattttgcaa attaaaataa
    19861 ttattatagc tttcttgata ggaagtagtt atttgatagg tgaacaaata tacaaaactt
    19921 atactcgtag acataaacaa ttaaacgatt taataagagt tttagaaatt ttgaggatgg
    19981 atttatcttt tggactttat acattagaag aaatctttaa taggataggt ggaaataaag
    20041 agttttgttt ttggaaattt ttctatcaaa tatcagaagg acttcacaat gaacaaagta
    20101 aaactttgga aataataata tcagaaaata ttgatgtatt aagtaaagaa acttatctag
    20161 gcaataaaga aattgaggaa ctgaaaaatc taatacttac tttagggaaa agcgatatag
    20221 agtcacaaca aagaatgata gatttatcta tagaaaattt gaaaaaacaa acctatgaga
    20281 caaaggaaga tataaacaaa aaaggcgtat tgtataaaaa attagtgaca ttcataggta
    20341 taggtatatg tattatattg atttaatatg gggaggttta ttatggaaat atcattgata
    20401 ttaaaggttg caggtgtcgg tattctaata tcagtattga atatgatatt ggaaaaaaca
    20461 gatagaaaag attgggcagg tcttactaca ttagcaggtg taataatcgt attgggtatg
    20521 gtaataacag aaataagtga cttgtttaat actgtaagaa caatgtttca actttactaa
    20581 agaggaggga gtaatttgga aataatgcaa ttaataggaa ttgcaatcat ttcaactaca
    20641 ctctgtttag taattaaaaa agatagacca gagatagcaa atttcatagc tataataact
    20701 ggagtaataa tactattgtc agttatgttt aagctaaatt ttatagtaga tagtatacaa
    20761 gatttggcta acaaggctaa catcccgact atgtatatat cattaataat aaaattaatt
    20821 gggatagcat acttaatgga atttgcgata caactttgta aagattgtgg ggaaggaaac
    20881 attgcttcaa aactagaatt tggaggtaaa ataatagtta tgtcaatgtc tttccctata
    20941 cttttgtcca ttgtagaaat ggtggtgaat ataattccat agatgtgtat cttggaatga
    21001 atataagtat attagagagc tttcgttgta ttgaggttta tagagtgaag agaggcatgg
    21061 ttgcttatga aaaaaagatt tttaccaatg attataggtt ttttatttac tttctttttt
    21121 gtaaatatct ttgctattat gatatttgct aatgaagtac caagtagtga agataaagaa
    21181 tatggtgaaa caaaaaatag catagataag tatatagatg gtcaattaga caagctagat
    21241 ataaatgaga ttcaagacta tataaataaa gaaatagtta ttaatgatgt taatttaaaa
    21301 tcatttgtaa aagatttaat tagtggagaa aaaaatatct tagatttatt caataaagat
    21361 ggattgaaga tattgatgtt tgatgaattt aaagcaagtt taaaggtagt agcagtaatt
    21421 ttggtactgg ctctgctatc atctatttta aagagtctgg aaaactcttt ttcatcaggt
    21481 gcagtaagtc aaatagcaac atatataata tttatcacaa tggtatcact tacacttatt
    21541 ggttttaaag atgtattaca gatttgctat gatgcaatag accatactgt aggtcttatg
    21601 caagttataa tgccaatatt aataacattt ttacttttaa ttggattccc aataacgtcg
    21661 actactttga atcctatctt cataggagga gtaacattta taaatgtatt ttttaaaaat
    21721 tttctatttg tatctattac tgttgcattt ggaattttaa taataaacaa tttatcaaaa
    21781 aatataaggc taaagagatt tttctccttt gtaaaacaaa taaattatgt ttctattgga
    21841 gctatgttta ctgtttactt aggtcttgta tccatacaag gactgtatgt aaccagtttt
    21901 gataaattca gtgtgaagac tgctaaattt gctataggta attttattcc tgttgtaggt
    21961 ggatttgtat ctgactctgt ggatatttta ttatcttcat ctcagcttat aaaaaatata
    22021 tttggaggaa tagggcttat cttacttgtt ggaatttgct tacttccagt aataaaaatt
    22081 ttatcagtaa tagtagtgta caaattagca gcaataattg tagaacctgt tggagaggat
    22141 ggaatatcta attttttaaa tgaagtggca aatttaatga taataatgct agcatcagta
    22201 attgctataa cagtaatgtt ctttgtaact gtagctatct tgacctcgat aagtgtcgta
    22261 agtcaagggt aatttatata tcagtgaaaa ataattacat ttaatctaat atttttagag
    22321 aggaggatgc tatgttagag ggcataaagg catggatagt tagtgttttg ataggagcat
    22381 ttatagtaaa tatagtagat atgattttac caagttctaa gataaaacct tatgtcaacc
    22441 tagttttaaa ctttatgttt gtatttattg taataactcc agtagtagga tttttttcaa
    22501 aagatatgag tttagaagat agaatattaa aatctatggg aaactacaac aagcagtatg
    22561 tagatagtac aaatgctttg gcaaaagaga ctggaaataa tagtctatcc aagggatatg
    22621 aggatgggtt aaaagaagta cttaaattaa aacttgatga atatggttat gatttagaag
    22681 atatagagct taatggggca aatataaata atataaaaat aaaagaaaag aataatagtg
    22741 caaaaagtaa taacagttcc aatgaagaaa ataaaaataa tagtactaaa agagaggaag
    22801 aaaacataaa ctctaatgac aaagaaaatt ctaaacaagt cttcaaaaaa ggtacagagt
    22861 atgggttgaa cctaaatgaa gaaaaattaa aaaacgactt aataaaggtg ctggatgttt
    22921 ctatagaaga tatacaaatt gataaatagg agttgggaga atgttgaaaa acttaaatga
    22981 caaagataag agaaaggtat attcactaat aactatagca ggaatatgtg tggtttcatt
    23041 agtattatta tcttgttttc ctagtagcaa aagcgataaa caagttggaa aaacagagac
    23101 taataaaaat gctgaaaagc aagtcactaa agaacaagaa aaagatgatt tagagtcaaa
    23161 attaacagca atactatcta aaattgatgg tgcaggagac gttgatgtaa tggttacatt
    23221 tgaatcaagt gaagaaatac aacctgcttt taattcaaat aatacaacag aaacaacaga
    23281 ggaaaaagat gcacaaggtg gagaaagaac agttacgact tctagtgaaa ataaaacaat
    23341 gataacttca aattcaagtg accctgtagt tataaaaact acagagccaa agataaaggg
    23401 agtgatagtg gtagcaagtg gagcaagtga cccaagtgtt aaagagacac tttatagtgc
    23461 agtacaaaca tcattacaag tagctggtca tcaagtagaa atatattcta aataaataca
    23521 tataaccggg gggataaatg aatgaagttt aattataagg gaagaggatt tgtaataata
    23581 actttaactg caatgttagt ggtagtagga acagtgaatt atcaattaag taagaaatct
    23641 ttattagaaa catcaaaaga gtttaaggca tatgaagaag cacagttaca aaaaaatact
    23701 gatgagagtg atagtagtaa gacagaggat tctagtaata tagataaaca agatggtaag
    23761 gaaagtgctg atattgatat agttgatagc aaagctagta aagtaaaaga aaaggcaact
    23821 gagacaagta aggaaataaa agctcaatta tcatcagaga aaaatatgaa aaaggcatct
    23881 tatattttag atatgaaaat gaatagagaa aaacaaagaa atgagttagt tcaagattta
    23941 aatgaaatga taaataatcc atctacaaca gaagaatcta gaaaagaagc ttctaatatg
    24001 aaattaaata tagttaaaat tcaagaaaaa gaacttcaaa tagaaaacct tttgagtaca
    24061 aaagggtatg aagaagcgtt agtttatata agcgataaca aagttaatgt agttgtaaat
    24121 gaagctaaat tagaaaagaa agatgcagca aaaatatttg atttagttgc agaacaagcc
    24181 aatgtgaagt atgaaaatat taaacttaca aataataata gtaataagta aagtataaac
    24241 ctcagaatta actgaggtta ttttcttgca aagtagactt gtatttggta ttattatact
    24301 tataaaaata gttccttaaa atcaggaggt aagttttaat atggaagata ataaatttgg
    24361 acaagtaaaa atatctaatg atgtaatagc tacaatagct ggtttagcag cattagaagt
    24421 tgaaggcata gaaactacag caacattaac agataagttg ttaaaaaata atggagtaaa
    24481 aatacaaatc gaagaagagg atgttaattt agatgtaatg gttacgataa agtatggaat
    24541 gtcaatacca gatacagctt ttaaagttca agaaaatgta aaaaatactg ttgagacaat
    24601 gacaggactg aaggtttctc aagtaaatat acacattcaa ggaatcagct ttaagaagga
    24661 taaagttgat aaagaagaag caaaagcaac taagaaaaat taatattagc cctctgaaaa
    24721 gtcagagggt tttttgctgg taattgattt tatatggata attaaactta aaggagaatt
    24781 ttgattgatg aaaaaagata gggcacaaaa gagtacaaca agagaatata taatgaaact
    24841 tatataccaa ataaatatta ataaagaaga ttttgaaact ttagaagata aagtagataa
    24901 ttttttaaaa gataattcag aacatattat aaatagatat aaagaacttg cacttcaata
    24961 ctcaaaaaat acaaatttaa agttagaaga tactgaaatt gaagatgtta tagacaaaaa
    25021 atatataaat acagtatgca aagcattgaa agaaaatcat gacaaaatag atgagttaat
    25081 aaataagcat gctaaaaact ggacagtaga tagaatgcca aaggtagatg tttctatact
    25141 tagattgtct gtgtgtgaaa tactttatct agacactcct aataaagtat caataaatga
    25201 agcagtagaa cttgcaaaaa tatattgtga tgataagtct cctaaattta taaatggtat
    25261 attgggaagt gttgttgatg aaattgggaa ataatataat aattggaatt gatactagct
    25321 gctatactac ttctatagca gctatctctt tagataagaa agttatatta aatgaaaaaa
    25381 taatgttaga agtaagggat aattcaaaag gtttaaggca aagtgaagct gtatttcaac
    25441 atatcaataa tttagggatt cttagtgata ggataaaatc ttttaaagat aagtttaatg
    25501 tagaaggagt ttgctcatca aaaaaaccga gacctgttga aaactcatat atgcctgtat
    25561 tcaatgtagg tcataatttt ggaaaattat tgtctagtat atatggttgt aggttttatg
    25621 agactactca tcaagagaat catattgaag caagtttatt aaatagtaaa ttaaaaaata
    25681 ataataaatt tatttctgtt catatgtctg gtggtactac agagatactt ttaacaagca
    25741 aacaagatag tcatcacaat gtatgtgata ctaatttagg taaaattgcc aaaataagta
    25801 ttaaaaaaga tgataaaagt aagttataca ataattttgg atataatata gatataattg
    25861 gaggaagtaa agatattagc tttggtcaac ttatagatag agtaggaatt aagcttggat
    25921 ataaatttcc ttcaggaaag tatttggatg aaaatgcttt aaattgtaat cttaaaatag
    25981 aaagtggtct gaaaacttct gtaagagatg ggtatatgaa tttatcagga ttagaaaatc
    26041 aggtaaataa gattataaat gataatggag ataatactaa tcaaaaagaa tatatatcta
    26101 aattggtatt agattcagta gttagaaata tgtttaaatc tttagtgtat ttatgtgaaa
    26161 cttataatgt aaatgaagta gtatttgctg gtggagtttc tgcaagtaag tatattctta
    26221 gagaattaag catgaagtta agaaaaaagc atatagaagc ttattttaca gaaccacaat
    26281 attcaactga taatgctgtc gggtgtgcta taataggatt gaataacttt ttaggagaaa
    26341 gagtatgaaa ctaagagctt tagatataag tgaagcaaac tcatatatta aaagaatttt
    26401 aataaacgac ccaatacttt ctaatcttaa agttaaagga gaaatttcta attttaaggt
    26461 tcatagtagt gggaatgtat atttatcact taaagatgaa acatcaaagt taaactgtgt
    26521 aatttttaaa agtaatttta atcgcaattt aaaattagat aatggtgtaa aaattatagc
    26581 taatggatat atatcagtct atgaaagaga tggtgcgtat caattataca ttaatgaaat
    26641 tgaaatagag ggaataggaa atttacacat agagtttaat agattaaagg aaaaacttaa
    26701 taaagaagga ttatttgacc ctaaatacaa gatacctata cctaaaatgc caaactcaat
    26761 tggcgttata acatctccaa caggtgctgt tataagagat ataataaatg taataaaaag
    26821 aagatatccg aaagttaata taaagcttta tccagtaacg gttcaaggag ataaatcagc
    26881 tgaagaaatc tgtgaagcaa ttagattttt caatcatatg aagaatgttg atacacttat
    26941 agttggtaga ggtgggggtt caatagaaga actttggtct tttaatgatg aaatggtagc
    27001 aagagaggtt tttaattctc aaattccgat aatatcagca gttgggcatg aaacggattt
    27061 tactatttgt gactttgtgt ctgatatgag agccccaacg ccatcagcag cagctgagat
    27121 tgctactcca tcattggatg atataaacta taaacttgga aacataaaaa gtaggatgag
    27181 taagtctctt acaaatcaaa tagagctaga ccaatatagg ttagaaacag tatttaataa
    27241 gataaataac tatttagatt cgtacacaat aaaagataaa gttatacaat tagacaaaat
    27301 atatgataaa ataatttttg gaatagaaaa taatttaaaa ttagaagatg aaaaattagt
    27361 aaaaattgga gcattactcc ataatttaag tccattagct actatggata gaggttatag
    27421 tataactcaa aaaaatggaa aagtaataaa tagtataaaa ggattaaaag taaaggatag
    27481 tatagatata gtattaaaag atggaaattt agaatgtatg atagataaaa ttgaaaataa
    27541 agagggatag tatgaattta acctacgaag aagcttataa aagattagaa agtattttaa
    27601 gtgaattaga atctaaaaat gctagcttgg atgaatcttt aagcctatat gaagaaggta
    27661 taagccttta taaacattgt aataagcttt tagatgatgc taaattgaaa ataagtaaat
    27721 ttaatcaatt gggaatagaa gaagacttta aaatagagga ggaataaaat tggaatttaa
    27781 acaatgtcta aaagaaaaag caagttttgt agaaaaagta cttaaagaat atatgcctaa
    27841 agaagaaggg tatcaaaaaa ctgttattga agctatgaac tatagcttaa gtgctggagg
    27901 taaaagatta agaccaatac ttacattgga ggcttgtaaa atagttggag gtaatgaaga
    27961 tgaagctatt ccttttgcta ttgcaattga aatgatacat acatattcac taatacatga
    28021 tgatttgcca gctcttgaca atgatgattt aagaagagga agaccaacaa atcataaagt
    28081 atatggagaa gctatgggga ttttagcagg agatgctttg ttaaactatg cttttgaagt
    28141 aatgcttgca ggctcaatca ataaagaaaa tccagaaaag tatctaaaag ctataaatga
    28201 aattgccaaa ggtgctggta tatatggaat gataggtggt caagtagtag atgtagaaag
    28261 cgaaaataaa caaattgaaa aagaaaaatt agattatata catatgaata aaactgctgc
    28321 aatgatggta ggctgtatga gagcaggagc tactataggt ggagcaaatt ctgaacaaat
    28381 ggaagaaata actaaatatg caaaaaatat cggattatca ttccagatag tagatgatat
    28441 actagacata gtaggagatg aagctaagtt aggtaaaaag gttggaagtg atatagaaaa
    28501 tcataaatca acatacccat ctcttttagg tttagataaa tctaaagaaa ttgcacataa
    28561 tttgattgat gaagcaaaga aaagtataga aaaattatct gatgatgtag attttttaaa
    28621 gggattggct gaatatataa tagatagaga atactaaaat atacagctaa aatcgaggag
    28681 ggaaaaatgg attttttttc ggagattttt aacaatgggg ctttgggaat aagtctgatt
    28741 gcttgtttct tagcacaatt tataaaaata tttactggta aggaaaaaag aattgagtta
    28801 tcaagaatac ttatttcagg aggtatgcct agttcccata gttcttttgt tacaagttta
    28861 gctacagtgg taggtattga aaaagggttt aattctactg attttgctat aattaccgtg
    28921 cttgccttaa ttataatgta tgatgcagca ggtgttagac gggctgttgg taaacaagct
    28981 actatattaa atcaaatggt ggcagatata cagcatggaa agcatataga gcagaagaaa
    29041 ttaaaagaat taataggtca tacaccacta gaagtttggt ttggagcttt acttggaata
    29101 gtaacagctt taatattgat gtaaattgaa aatttacaga ataaaggtga ggataatatg
    29161 tataaatatt tagataaagt aaattctcca aaggatataa aaaatatgag tatagaagaa
    29221 atggatttac ttgcaaagga tataagaaag ttcttagtaa aatctgtttc aaagacagga
    29281 ggacacttag cttctaatct aggagtggtt gagttaacat tagcacttca taaagtattt
    29341 gatagcccta aagataagat tgtatgggat gtagggcatc aatcttatgt tcataaaata
    29401 gttactggaa gaaaagattg ttttgtatct ttgagacagt ttaatggact aagtggtttt
    29461 ccaaaggaga atgaaagtcc tcatgatatt tttgatacag gacatagcag tacatctatt
    29521 tctatagcta caggtattgc ctgtgctagg gatataaaaa aagaaaacta tagtgttata
    29581 tctgtgatag gagatggttc tataacagga ggtatggcac ttgaagcttt aaatcaatta
    29641 ggatatatag ataccaatat gatagtaatc cttaatgata atgagatgtc catagataaa
    29701 aatgttggag gaatgtctaa atatctgtca agtattataa gaaactcaac agttgagaaa
    29761 atgacagatg aagttgataa aattttaaat gttacacaaa caggtgaaat tttatctaag
    29821 actgcacata gatttaaaga taaactaatg tatagctttt ctcctcagga ctgttcattt
    29881 tttgattcat taggtataag atattatgga cctatagatg gccataatac taaagaacta
    29941 atagatatat tgagaaaagc taaacacaaa aaagggcctg ttcttttaca tgtaattacg
    30001 aaaaaaggaa aagggtacag atttgcagaa gaacaacctg ataaatatca tggagtatca
    30061 aagtttgata taaagacagg tgttacatct gcaaaagtta agtctatgtc aattagtgtt
    30121 ggagaaaaac ttgttgatat ggctaatagt aatgaaaata tagtagcaat aacagctgca
    30181 atgccatctg gtacagggct aaacctgttt gaaagtgcat atccaaaaag atattatgat
    30241 gttggtatag ctgaacaaca tgcaacagga tttgcagcag gtcttgcaaa aaatggtatg
    30301 aaaccttact ttgctgtgta ctcatcattt ttgcaaaggg catatgacca agttattcat
    30361 gatgtatgca taacaaaaaa gccagttact tttcttatag atagagctgg attagttgga
    30421 aatgatggag aaactcatca tggtatgttt gatttaagtt acttaaattc tattccaaat
    30481 attgtagtaa tggctccaaa agacacaaga gagatggagc ttatgatgga tttgtcatta
    30541 aaattagatt gtccactagc aattagatac ccaagaggaa gtagttatta cctagataag
    30601 ggagagtatg gagagattgt actgggtaaa tatgaggtat tagatgacgg tcaagataca
    30661 gtcatacttt gtattggaag tatggtaaaa catgctttag aggctaaaga aatattatca
    30721 agagaaggaa taaatccgac aatagttaat gctagattct taaagccaat tgatgaaggt
    30781 atgttaaaag cattattaaa aaatcataaa aatgtagtta ctatagagga taatattgta
    30841 actggaggat ttggaagtag aataaataag tttattatag ataatgaata taacgtaaat
    30901 atacttaata tagcaatacc agaagaattt gtaaaacatg gcaatataga tgaattatat
    30961 gattttgtag gattatcgcc aaaaagtatt gctgataaga taaggaaatt agttattgag
    31021 taaaagtcta atgtagatat tgtaaattaa aatttatata aaatatagaa tttatcaatt
    31081 agaataaata tcaagtgtgt tacttcaagc tactttagtt atcattaaag tagcttttaa
    31141 cagaaaggta attgtatgaa aaaaagaata gatttattat tagtagaaca agggtacttt
    31201 gaaagtagag aaagagccaa aaaagcaata atggctggat tggtttttgt tgataatcaa
    31261 aggtgtgata aggctggtac agaagtaaaa gaagattgta gtatagaagt aaaaggaaat
    31321 ccaataccat atgtcagtag aggtggttta aaacttgaaa aagcaatgaa aaactttgat
    31381 ttgactattg atggaaaggt atgtatggat ataggggcat ccacaggtgg ttttacagat
    31441 tgtatgctca aaaatggagc aataaaagta ttttctatag atgttggtta tggtcaactt
    31501 gcatggaaat tgagacaaga tgatagggtt gtgtgtatgg aaagaaccaa tataagaaat
    31561 gtgacaatag aagatacaaa gcagtttgct gattttgcgt caattgatgt gtcttttata
    31621 tcgttaaagt tagtacttcc aaaggcaaag gagttagtac gtcatgatgg ggaagttgtc
    31681 gctttaatta aacctcaatt tgaagcagga agagaaaaag taggtaaaaa aggagtagtt
    31741 agagaaaaat ctacacatat agaagttata aaaatgattt ctgatttctc agtcgaaaat
    31801 ggatttgaga ttttagggtt agatttttct cctattaaag gtcctgaagg taatatagaa
    31861 tatctaattc atttgagaaa tggaaatgaa ggatatgaat ttgatggtga gacatacaat
    31921 aacaaaatag tagaagttgt tgaagcttct cataatttag acaaataggt taaaaggggg
    31981 cttagatttg atcctcgaat tatatatgaa aaattgtgcc ttagttgaag aactaaggtt
    32041 gaatattgat aaaaacttaa atatacttac aggtgaaact ggttcaggaa aatctataat
    32101 aatagatgca cttggacttt gtttaggtga taaatatgat aggtcttttt taagaaaagg
    32161 aacagataag ggacttgttg aggctgtatt tttttcagac aatagatatc ttaaaaaaat
    32221 attagaagaa aatgatataa gtatggaaga tgataattta ttggtaataa ctaggttgat
    32281 atactcagat ggtaagagta ctgctagagt aaatggaagg actgttaaag tgtctcttct
    32341 taaagaaatt gcatctacac ttatagatat acatggtcaa catcaaaatc aagctttgtt
    32401 taataaagat actcatttaa aatttctaga cctttttgga gaaaatgaat tggaagagtt
    32461 taaaattgct tataaaaagg tgtaccataa gtatagtgaa gtaaaaaaag ctttaaattg
    32521 cttgacagaa aataaagatg agatgcaaat tcaacgagaa atagatttat taagatttca
    32581 aataaatgaa attgaagctg caaatctaaa taagaatgaa tatgaagatt tattaaaaca
    32641 aagagaagtt tatagaaata gtgaaaagat atataataat ctaaattcga gttatagcaa
    32701 gttacataat ggtgagtata atgttattga cctaataggt cttgcatcta aggaacttaa
    32761 tgacatatct aaatatgaca gtgttttaag tgaatatagt gatactgtag aaagaatcat
    32821 gtatgagtta caagacatat ctggtgagat aagaaattac aaagataata tagactttga
    32881 accttatgag ctagagcaga ttgagcttag aatcgacgag attaataact taagaagaaa
    32941 atatggagac agtatagatg atatatttga gtattatgga aaaattaaag atagattaga
    33001 tgaaatatta aacagagatg aaagagttga acagttaaga agcaaactga tgaatataga
    33061 agaagattta aaaataaaag ccagtaaatt gactaaagca agaatagaag tagcaactac
    33121 tttagaaaaa atacttcttg atgaattaaa aagcttaaat atgaaaaatg taatgttcaa
    33181 ggttaatttt gaaaaaggtc catttacttt agatggagta gatgatattg aatttatgat
    33241 atcttttaac ttaggagaag atattaagcc tatatataaa gttgcatcag gtggtgagat
    33301 gtccagattt atgttagcgt ttaaaaccat attggctgat atagatgata ttgatacatt
    33361 agtgtttgac gagatagata ctggaataag tgggatagca gcacagatag ttggtgagaa
    33421 attgagtgat atagcaaaga aaaaacagat aatatgtata actcatttac ctcaaattgc
    33481 tgcaaatgca gacactcatt attgtataga gaaagatact tcaaataata ggacgtttac
    33541 caatgttagt aagttaaatg aatctcaacg aaaaaatgaa attgccagac ttatagcagg
    33601 taataatata acagaaaaaa ctattgaaca tgcaagtgaa atcattgaat tggctaaaaa
    33661 atgttagtga aaaacaagca ttttaatagg tgtaaagaat ttttatagaa atgctttaca
    33721 cctatttttt ataatgaaat tattttaaaa tatctagtgc ctatattgaa gaaaagtagg
    33781 tttgaaaatg gaataaaatt tgtataactt tgtaactttg aaatttctaa atgcttaagt
    33841 ataatattat ttattaaggt agtagtgagg ggaggcataa aatgaataaa aaagatacta
    33901 tgcacttaat ttttataatt gtaattttta ttttattatg gtatattgtg gtagcagtaa
    33961 gtgagattaa taaaaatatt gaaagttgta gtaacaaatt agattatttg tcacaagagc
    34021 ttatgagtac taagtcggat attagtaata ctataaatga agaaatgtca aaaagttata
    34081 taacaaaaag tattgactta aaagtaaaaa aaatcgagaa aaaagaatgt attattgatg
    34141 tagatgttca attaagtaaa cttggaaaaa atggaaaagt attttttatg tataaagaag
    34201 atagtgataa atggaatgaa actgagatga caaaatatgg agaattatca tatgcatgtg
    34261 aaataaagat aaatacagga agtgaatatg actataaagt agttacaagt ggagctatat
    34321 ctgaaagttc tgatgtgcaa aaatttacta aatctgatta tatgcctgaa caacccatat
    34381 ttaattctgg gataagagat aatagagaat actttattga aatagtagaa aatagtaatc
    34441 ttttcaatca tgagagtgaa aagtcaaaaa ataaagctta cgataatatt agattagaaa
    34501 aagtagatat tattgtaaat gaaggaaaaa aagacactat atacaaagcc aaattagctg
    34561 aaggcttaga tgatggcaaa actaataatt caaatagaaa tcaagtgaat tatgaagtaa
    34621 gttttcctaa aagggatatt gatgacataa tttatattaa agctaaactt acatacaata
    34681 atggagtaga atatacaaag gatataactg aagatataac tatgggatta caagatttag
    34741 aaaaataaat acatatttaa ataaatattc aaatagattt gttcaataaa atagagtata
    34801 aataaaactg tagtagtttt acttatactc tattaaaaaa ttataatttt aattccatat
    34861 atctagcccc ttcaattgga ttataagtat aaggttctat atcataaaaa ccaaactttt
    34921 catataatcc ttgtgcactt ttcatgcttg gaagagtatc taatctcatg tgtgtatatc
    34981 caaatttttt agcttcttca atgatttctt ccaatagtat ttttcctatt tttaatcctc
    35041 taaattgatt tcttacatat aatcttttta attcacatat tttaccttca agttttttta
    35101 aagcaacaca acctgccaaa ttttcatcaa caaatgccaa tatcaatgag cctgaaggtt
    35161 ttttatattt tcctggaagt gtttttaatt cattattaaa gtcttgaaaa cataagtcta
    35221 tatttagaga atttgaatat tcagtaaata gaagttttac attttcaagg ttttctaaat
    35281 ctttttcttc aacaaattgt aaagttatat tttgagccat tttatccgcc attatataca
    35341 catgtttata taatgacttc acctccttga tatatgtata tttttcttaa tatcaattag
    35401 attttaatac cattactttt tcaccaccat agtcaagctc tccattaaac tcaaatccaa
    35461 aatctttata aagcttaata gccattttat tgtcttcgaa tatactaaga tatagttcat
    35521 cacagttata ttcattttta agatgattta caagaaaatc taatgatgct tttccatatc
    35581 ctttaccttg atattttgaa tcaatcataa atctatcaag ccaaactcta tcatcttcta
    35641 aacgaccata catagcaaat cctattaaag aattttcatc atatattcca acaggtaccc
    35701 aagctgttat aaatttagat tcagcaatag aaagtgcatt gctttctata taatctaact
    35761 gacttttatc tacagacaaa ctaactaaat catgccaatt atttatgtct gcaattctta
    35821 catttaattt caagatatta ccgcccttca aactttttgt tacaatctaa tactatcata
    35881 ttatgtatta aaaatcactt attaagattt taaataaact ttttaaatga ataaattctc
    35941 aggaaaaagg aattataaat acataataat acagcatagg agtagatgga tatatgaaca
    36001 aaataaataa aaatttcaaa aatatttgga taatattagg agctgtatcg atttttacca
    36061 tggttataat ttttaataaa aattgtaatg ttaataaaga gactcaaata gaagcttttg
    36121 caattaagaa tcatgataaa aaatttgtat atcctatggg aaatataatt ggagtaaaag
    36181 caaatacaga tggtgtattg gtcttaggat atgaggaaga ggatgtagac tatattggag
    36241 gcatacaaat cggtgataat atagtaaaaa tagataataa aaggataaaa aatagtcaag
    36301 atgtatcaga gattttgaat aagattaaaa aaagtaaagt tgaagtgact tttgagagaa
    36361 aaaataagta taaaacagaa gtaatagaga ctaaaaaaga aaatggcaag tataaacttg
    36421 gattatgggt aagagataaa atatctggaa ttggaactat gactttttac gaccctagta
    36481 tggaaaagtt caaagcaatc ggtcatgcca taaaagattc ggatactaat gaactattaa
    36541 agataaagca aggatatata tataagccag aacaattaaa aatagtaaaa gctagtaatg
    36601 aaaaagtagg taaaataaaa ggagatttta atgacagcaa tttaatgggt aatttctcaa
    36661 ataattcaga gctaggtata agtggtaata ttacagaaaa ccataataaa gagtttaatg
    36721 tagcaaataa agaaaagcaa ttaatagaag ttggaaggcc gcaagatgta aaaataggag
    36781 atgcagtaat tctttttgag gataaaaaca aaaatataac aagctatgat ataaaaatag
    36841 aaagtatagt atatgataaa ggaaattata gagatatggt aataaaagta gtagatgaca
    36901 agttattgga atacacagga ggtatcgtac aggggatgag tggagctcca ataatacaaa
    36961 ataataaaat tattggtgca ataactcatg tttttagaga taatccgaaa aaaggatatg
    37021 gtatttttat agatgaaatg ataaaattgt aggtgaggca ttaaaaaatt ttattatttt
    37081 atcaattatc taggaggaat ataattttgg agtgtcgaat atgctttaga gtagataatt
    37141 aggaagcaat tgtgtaaaaa gtttagtttt ctgtaataag aagatgtttt ttaatggggg
    37201 gatttttagt ggaaaaaatc aaaatagttt tagcagatga caataaggat ttttgtcagg
    37261 tattaaaaga gtatttgtct aatgaagatg atatcgatat attaggcata gctaaggatg
    37321 gaattgaagc attagactta gtaaaaaaga cacaaccgga cttattaata ctagatgtaa
    37381 taatgccaca tctagatgga ttaggtgtaa ttgaaaaatt gaatactatg gatataccta
    37441 aaatgccaaa aataatagta ctatcagcag taggtcaaga taagataact caaagcgcaa
    37501 taaatctagg agcagattac tacatagtaa agccatttga ttttgttgtg tttataaaca
    37561 gaattagaga gctagtttct aatagagtta cacaagttga gccaaaacct agaccagttc
    37621 aagagactca aatgactaga agtgattttg ttaagaatgt aggaaatata gagacagaaa
    37681 tcacaaatat aatacatgaa ataggagtac cagctcatat aaaaggatat ttatatttaa
    37741 gagaagcaat aaaaatggtt attgataatg ttgagctttt aggtgcagta acaaaagagt
    37801 tatatccaag tatagctaaa aaattcaata caacaccaag tagagttgaa agagcaataa
    37861 gacatgcaat agaggttgca tggagtagag gaaaagttga cacaataaat caattatttg
    37921 gatatacggt acacaatact aaaggaaaac caactaattc agaatttata gcaatgattg
    37981 ctgataaatt aagactagaa catagtatgg ttaaataaac aagacataaa aagtaaggct
    38041 ttttaattaa ggcattggct ataaatgcgt attacaagca gcgaaacggt ataaccacta
    38101 gggttatacc gtttcgctat tttaataaat ataaaaattt tctttattat ttgcttacta
    38161 tatcaatata ataattttat tatactatgg atatagtatg tgtctttaca agttgtaaac
    38221 tgacagtggt ttatttttta atataaatat tgactttgat gcaggtaaac tttgtatttt
    38281 taagcgtatt atggaatatg ttaaataaaa aaatgatgaa atatagtatt gtaaatgcca
    38341 aagatgcaaa acaaacttaa aacatttatt ttattgttaa gtaatgctat aatataatgt
    38401 gattttaata atgatagtgg aggtttaaat atgagagtcg aggcccctat aaaagtagat
    38461 cgaaaaacca aaaaacttgc taaaagagtt gaaagtgggg aaatagcagt tataaatcat
    38521 atagacatag atgaagttgc tgcaaactct ttagtagaag ctaaaataaa acttgtcata
    38581 aatgcggctc cttctataag tggtaggtat cccaataaag gtccagggat attaactgaa
    38641 aataatatac taataattga taatgttgga gaagagttat ttgaagaatt aaaagaagga
    38701 gaaactatag aagttgtaga tggaaagata tatagaaaag gaaaattttt aggtgcagga
    38761 gaagttttag acaagtatga ggtttcacac caaataaagg ctgcctatga gaatttagct
    38821 gtagaattgg atagatttat agataatact atagattatg ctaaaaaaga aaaaggattt
    38881 atacttggag aagtagaaat tccaaaagtg aaaactaatt atgctaataa acatgtattg
    38941 atagttgtaa gaggtcagga ctacaaagaa gatttaagta ctatgctttc ttatatagaa
    39001 gaggtaaagc cagtattggt tggtgttgat ggaggagcag acgctttaat tgagtttgga
    39061 tatactccag atgtgattgt aggtgatatg gatagtgtaa gtgatgaagc tttaaaaaag
    39121 gctagtgaaa tagtagtaca tgcttataca gatggaagag caccaggtct taagagggtt
    39181 gaggaattag gcttagatgc agttgtattt cctgcgccag gaactagtga agatatagct
    39241 atgcttatcg cctatgagta taaggctgag ttaatagtag cagttggtac acattcaaat
    39301 atgattgatt ttcttgaaaa aggaagaaaa ggtatggcaa gtactttttt agttagacta
    39361 aaaataggtt caaagcttat agatgctaaa ggcgttaatt tgttatacag aagtaaatta
    39421 aaaataaagt acatatgggc attaatagca acagcattat ttcctgtgtt ggtagttgca
    39481 tcattgtcac caggagttca acagttcata caactaatgc aattaaagtt tagagtatta
    39541 ttacagatgt aaattttagg aggataaata tgcatattaa tatgaaatat tatatagtca
    39601 ctataggggc tatatttata gctttgggta taggcatgtt agtaggattt aatttaaata
    39661 ataatcaaca attaagtgaa caacaagcaa atataataaa tgatttggat gataagtttg
    39721 atatcttaaa agaaaaaaat gataaacttg acagtgactt agcaagtgta aatagagatt
    39781 atgaagaggc agttaatttt ataaataaaa atgtagataa gattttagct ggttcgctga
    39841 atggtaaaag tataggaata atatctgcta atgaaaatga cgattataca aaaaatatag
    39901 aagacataat aaataaatct aatggaagca ttgcatttaa tataatatta aaagaaaaca
    39961 taacaaatcc agaaaagtta aaggaaatgt caactaaact aggtacagat gtaaaaaatg
    40021 caaatgatgc agttaattat ataattgaca ctcttaaaaa ggaagatgca agtgacattt
    40081 taacatatct tcaagaacta gatgtaataa agtttaattt tattggagat acatatctaa
    40141 aatatgattc cgttgtaata gctggtggaa atgatgcaaa agacagtaca aaacagtttg
    40201 aaaaaataga aaaatttgta gtttcaaaac ttaaatcaga gaataaatat ttagtagaag
    40261 ttcaaaatac aggagttaaa acttcttatg tagaactata ttctaaaaac aaagttgcaa
    40321 ctatagacaa tattgatgaa ggtataggaa gtatatctct agcgattctt ctacaacaag
    40381 gtaatatagt tggtaatttt ggtagattgg atactgcaac ttcattgttg ccatctatta
    40441 aatagaaata attttaagga gagaaaaaat ggattcttat ataagtataa taataccagc
    40501 ttataatgaa gaaagtagaa taaaatatac attagaaagc atattggaca ttaagcaaat
    40561 aaatgaaata atagttgtgg atgatggttc aattgacaat actgctaagg ttgtatctga
    40621 gctagaaaat gaaaagatta aattttttaa gttagataaa aatagaggta aaggatatgc
    40681 tttaaattat ggtatcaaat tagcaatgaa aaatgcagat attataggtt ttctggatgg
    40741 agatttagga agttctgcta aagaagttga aaaattaata gtacctatat taaatggtga
    40801 ggcagatgtt acaatagcca agtttcctcc tgcaaagaga aaaggaggtt tgggatttgt
    40861 aaaaggttta gctaaaaatt ctgtgtttga gatgactggt gttgagttag atgcaacttt
    40921 gtcaggtcaa agaatattta aaagagaagt tttagaaaag tttgatgaaa taccttttgg
    40981 atatggagta gaagttggaa tgaccattga tatattaaag tatggattta aaattaaaga
    41041 agtattagta aatatgactc ataatgaaac aggaagaaat ttaaaaggtt ttattcatag
    41101 agggaaacag tattatcaca taaaaaaggt tttaggacaa aaaaagaaag aatggaggta
    41161 atatcttagt gaaagattat atactttata ccattttatt attaactgga ttatttggaa
    41221 cctatgctgt aattccacta tttaaaaatt tattgataaa tggaaatgta cttagaccaa
    41281 actataaaaa agatatgata ccagtaagta tgggaattgt atttttgcct atgattgtaa
    41341 tcaatggaat aatacttgct ttctttacta cagaatttaa ggatttagcg tatatattta
    41401 tgtttatgtt tggtatgata tccatgtttt ttgcagggat actagatgat gtcattggaa
    41461 atagagatgt aagtggttta aaggggcatt ttaaaagttt atttaaagga agtcttacaa
    41521 caggaggttt taaagcttta tttggtggat ttgtagggct tatagtttct gttgctatat
    41581 caaaagacat aattgatata atagtaaata cactaataat agctctatca actaatttaa
    41641 tgaatctttt ggatttaaga ccaggaaggg caataaaagc atatttattc ataatggtaa
    41701 ttatattttt aacattgaca ggatttgttc aagttcttcc attacttata gtaccaaatg
    41761 tacttgcata ctttaattat gatttaaaag caagggctat gatgggagat actggttcaa
    41821 acgtccttgg aatatctatt ggaatgctca tttcttttgg gtatccattt aacattagat
    41881 taggatggtt aatatttgta gttattatgc atatattcac tgagaaatac tcattaacta
    41941 agataataga aaaaaataaa ctattaaatt ttatagataa attgggaagg tagtattatg
    42001 ataagcaaaa gagtggggat tgttgaatca atagtaagtc aaactgaaac tctagatgat
    42061 ataagagtaa atataaatgg agaaatacaa agggcatata attatcccaa aatatctgga
    42121 actataaata taggtgacga agtggtttta aatacaacag cagtagaact gagcttaggt
    42181 acaggtggat accattttgt aataactaat ttaaataata tagaatctac tttaactgaa
    42241 ggtggacata taatgaaact tagatatacg cctcttcaaa taaaagtgga ttctgttgag
    42301 gaacaagaaa gtatttatca tgataaattt gcgaatttta aaggattaga aggtctttct
    42361 gttgtagtag ggactcttca tagtatgtta actccatttg cagcttcatt taaaagaaat
    42421 aatccaaata aaaaattagt gtatataatg actgatggag catctcttcc aatttattta
    42481 agtaaaaatg tggatacttt aaaagaaaaa aaattaatag atagtacaat aactatagga
    42541 aatgcgtttg gtggagatta tgaatgtata aatatatata ctgcattaat tacagcaaaa
    42601 gaaatactaa aagcggatgc agtatttgtc agtatgggac ctggaattgc tgggacagga
    42661 acaaaatatg gatttacagg gattgaacaa ggctctattc ttgatgccgt aaaaaaactt
    42721 gaaggaagag caattgcaat tccaagaatt agttttgctg acaaacgaga aagacatcaa
    42781 ggtatttcac atcatagtat gactgtattt aaagaaattg taaatgtaaa tgtagacata
    42841 ccaataacta tatatgatga tgaaaaattg aactttataa aagagcaaat caatttaaat
    42901 ggtttagaca aaaagcataa tataatttat atagataata ataaaacgaa agaggattta
    42961 gactatttta atctaaaagt aaagagtatg ggaagaaatt atgaacaaga tgaggcattt
    43021 ttcaaagctg caagcacagc agcatattac ttaatggagg tttaagatgg tattagaaga
    43081 aaaaactatt agtagtgata gggtttatac aggtaaagta attactttaa aggttgatac
    43141 tgttgaaatt ccagggcaag gatatcaaaa aagggagtta gtagaagttg gtggagcagt
    43201 aggaatagtt gcaatcacag atgataataa agttgtgtta gttaagcaat ttagaaaacc
    43261 tatagaaaag ccaatctttg aaattccagc tggtaaacta gaaaaaaatg aaagtccaaa
    43321 agaatgtgct gaaagagaat taaaagaaga aactgggtat agtgcaaaaa atattaagct
    43381 aatacataaa tttttcactt cagcaggttt ttctaatgag ataatgtttg tatatttagc
    43441 aacaggtctt actccaggag agaataattt agatgcagat gagtttttag atgtctatga
    43501 aattgagctt gaagaagcat ataacatggt attaaaaaat gacgttgaag atgcaaaaac
    43561 atctattgga ttattattag taaaagatat gtttaaaaat taatatttat aaaatgaata
    43621 accctttgtc ctaaagcata ctattttata aaggacaagg gggaattaaa ttgagaagaa
    43681 catatagaaa aaatgatttt agagaattaa atagacaaat aattattata ggattgttgt
    43741 ttatgatgtc tattgttatt gggtcttaca taaataaaat attgccaggt tctagtaaca
    43801 atatattaaa caatataaat cctgctgtag agtattataa tctaaatata agcataaaag
    43861 atacagtgat acaaaattta aaatcagatg caatctttat gggctcaata gctctattaa
    43921 gtctatttgt agtaacaatt ccagcagtgt tggtagcttt tgtattaaaa gggatgtcaa
    43981 ttggttatac aataaatagc tgtatattgg cattaaaatt taaaagtata aaaatgattt
    44041 taataatatt atttaaaaat ttgattatta ttccatgtgc aatcatatta gcattaatta
    44101 gtttaagtta ttttaaagaa atggtttatg aatttaaaaa gaaaaataga aaaaatatgc
    44161 aatttttaat taagaggtat atactaaata ttataattat aatagcacta tcattggggt
    44221 tgcaattaat attaaatact gcaagtattg gtattattaa gttcctagct aaataaagga
    44281 gtttataatg gatattatag agggatatat agattacata aa
//
LOCUS       DAENCV010000020        44113 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAENCV010000020 DAENCV010000000
VERSION     DAENCV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.020"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            369..1208
                     /locus_tag="KQD82_001043"
     CDS             369..1208
                     /locus_tag="KQD82_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG4379525.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1205..2386
                     /locus_tag="KQD82_001044"
     CDS             1205..2386
                     /locus_tag="KQD82_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379526.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2690..3592
                     /gene="ptb"
                     /locus_tag="KQD82_001045"
     CDS             2690..3592
                     /gene="ptb"
                     /locus_tag="KQD82_001045"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG4379527.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3614..4693
                     /gene="buk"
                     /locus_tag="KQD82_001046"
     CDS             3614..4693
                     /gene="buk"
                     /locus_tag="KQD82_001046"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG4379528.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4683..5360
                     /locus_tag="KQD82_001047"
     CDS             4683..5360
                     /locus_tag="KQD82_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4379529.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5403..5618
                     /locus_tag="KQD82_001048"
     CDS             5403..5618
                     /locus_tag="KQD82_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4379530.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5647..6726
                     /locus_tag="KQD82_001049"
     CDS             5647..6726
                     /locus_tag="KQD82_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG4379531.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6726..7478
                     /locus_tag="KQD82_001050"
     CDS             6726..7478
                     /locus_tag="KQD82_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG4379532.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7479..8036
                     /locus_tag="KQD82_001051"
     CDS             7479..8036
                     /locus_tag="KQD82_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG4379533.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8206..9552
                     /locus_tag="KQD82_001052"
     CDS             8206..9552
                     /locus_tag="KQD82_001052"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG4379534.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSLPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATDLANLIQEKLS"
     gene            9819..11651
                     /gene="glmS"
                     /locus_tag="KQD82_001053"
     CDS             9819..11651
                     /gene="glmS"
                     /locus_tag="KQD82_001053"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG4379535.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12103..13398
                     /locus_tag="KQD82_001054"
     CDS             12103..13398
                     /locus_tag="KQD82_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG4379536.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVRRGGHIKGAVDFSANWLSVYLDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13854..14591
                     /locus_tag="KQD82_001055"
     CDS             13854..14591
                     /locus_tag="KQD82_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG4379537.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14612..15865
                     /gene="murA"
                     /locus_tag="KQD82_001056"
     CDS             14612..15865
                     /gene="murA"
                     /locus_tag="KQD82_001056"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG4379538.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15952..17016
                     /gene="spoIID"
                     /locus_tag="KQD82_001057"
     CDS             15952..17016
                     /gene="spoIID"
                     /locus_tag="KQD82_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG4379539.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17082..17750
                     /locus_tag="KQD82_001058"
     CDS             17082..17750
                     /locus_tag="KQD82_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG4379540.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17848..18108
                     /gene="spoIIID"
                     /locus_tag="KQD82_001059"
     CDS             17848..18108
                     /gene="spoIIID"
                     /locus_tag="KQD82_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG4379541.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKENKKM"
     gene            18305..19324
                     /locus_tag="KQD82_001060"
     CDS             18305..19324
                     /locus_tag="KQD82_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG4379542.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19340..19768
                     /gene="fabZ"
                     /locus_tag="KQD82_001061"
     CDS             19340..19768
                     /gene="fabZ"
                     /locus_tag="KQD82_001061"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG4379543.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19879..20388)
                     /gene="yyaC"
                     /locus_tag="KQD82_001062"
     CDS             complement(19879..20388)
                     /gene="yyaC"
                     /locus_tag="KQD82_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG4379544.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20577..20680
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20817..22010
                     /locus_tag="KQD82_001063"
     CDS             20817..22010
                     /locus_tag="KQD82_001063"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG4379545.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22326..23423
                     /locus_tag="KQD82_001064"
     CDS             22326..23423
                     /locus_tag="KQD82_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379546.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23687..25906
                     /locus_tag="KQD82_001065"
     CDS             23687..25906
                     /locus_tag="KQD82_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG4379547.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDT
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEILKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVEKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25909..26691
                     /locus_tag="KQD82_001066"
     CDS             25909..26691
                     /locus_tag="KQD82_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG4379548.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27023..28936
                     /locus_tag="KQD82_001067"
     CDS             27023..28936
                     /locus_tag="KQD82_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4379549.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29077..29385
                     /locus_tag="KQD82_001068"
     CDS             29077..29385
                     /locus_tag="KQD82_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4379550.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29408..29734
                     /locus_tag="KQD82_001069"
     CDS             29408..29734
                     /locus_tag="KQD82_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4379551.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29773..31107
                     /gene="celB"
                     /locus_tag="KQD82_001070"
     CDS             29773..31107
                     /gene="celB"
                     /locus_tag="KQD82_001070"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG4379552.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            31118..31636
                     /locus_tag="KQD82_001071"
     CDS             31118..31636
                     /locus_tag="KQD82_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG4379553.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31732..32691
                     /locus_tag="KQD82_001072"
     CDS             31732..32691
                     /locus_tag="KQD82_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG4379554.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32740..33792
                     /locus_tag="KQD82_001073"
     CDS             32740..33792
                     /locus_tag="KQD82_001073"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG4379555.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33795..34469
                     /locus_tag="KQD82_001074"
     CDS             33795..34469
                     /locus_tag="KQD82_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG4379556.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35022..35573
                     /gene="raiA"
                     /locus_tag="KQD82_001075"
     CDS             35022..35573
                     /gene="raiA"
                     /locus_tag="KQD82_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG4379557.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35705..38380
                     /gene="secA"
                     /locus_tag="KQD82_001076"
     CDS             35705..38380
                     /gene="secA"
                     /locus_tag="KQD82_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG4379558.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38395..39502
                     /gene="prfB"
                     /locus_tag="KQD82_001077"
     CDS             join(38395..38469,38471..39502)
                     /gene="prfB"
                     /locus_tag="KQD82_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG4379559.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39646..41787
                     /locus_tag="KQD82_001078"
     CDS             39646..41787
                     /locus_tag="KQD82_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG4379560.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41935..43101
                     /locus_tag="KQD82_001079"
     CDS             41935..43101
                     /locus_tag="KQD82_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4379561.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43161..43278
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43403..44008
                     /locus_tag="KQD82_001080"
     CDS             43403..44008
                     /locus_tag="KQD82_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG4379562.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT        17855 a         4719 c         8430 g        13109 t
ORIGIN      
        1 tgataagtaa tatttagatt ttgtatttgt ttatagttaa taagtggatt atagaattta
       61 taataattaa tatatattaa acaaagatat attaagatat tttagaacac aaaactgcat
      121 taaggcgcga gtactatttt gtattcgcgc cttgatggtt tgagttattt gagtaaaaac
      181 taactttaat aagaaatctg tatttattaa gttgatattt ttaatgtatt tagagattat
      241 ttaacaatct ccaaatataa aactagaatt atttacataa aagttaaata gtttgtatag
      301 agttagattt atatagtata atacttagtg taaataatta agaagttatc aaacaggagg
      361 ataaaactgt gttagatata aattcttttt taaatggatt ttttaatata atattaagaa
      421 tgagtatata tgatttaatt gacatatcta tagtagcgta tattttttac aaaatattta
      481 tgtttataaa agatacaaga gcagaacaag tgtttaaagg tataatattt cttttattag
      541 caactcaact tagtaacacc tttaaattgc ataccgttta ttggatatct cttaaagctt
      601 tagattatgg agttatagct gctcttataa tttttcaacc agaatttaga gcaggacttg
      661 aacatatagg aagagcaaag tttaatttat ttggaaagaa tgtaaatact tctgaagaaa
      721 ctctcaatag aaatatagaa gaaattgtag aagctttata ttcactttct agacaaaaaa
      781 taggagctct tatcataatg gagagagaaa ctagaataag tgatataata aatacaggaa
      841 ctattattga tgctgaaatt tctaggcaac tattaataaa tatatttata ccaaatacac
      901 cattacacga tggagctgtt gtaattagag attctaaggt aaaagcagct gcatgttttt
      961 tgccattgac agaaagtaag gatttaagta aagatttggg tactagacat agagcaggaa
     1021 taggggtaag tgaggtgtct gactgtataa ctttgattgt atcagaggaa acaggtggcg
     1081 tatcaattgc taaagcaggt aagttatata gagatatttc tagagaaaga atgatgaata
     1141 tattacgtag taatttaaaa acaaatacag aaacaagaag ttttttcaaa ggtggtatat
     1201 ttaaatgata aatagactaa aaaataatac caaaataaaa ataatatcat ttttaagtgc
     1261 aattgcatta tggatgtatg ttatggctgt agttgaccct gaagaaacta aagggtttga
     1321 agatatacct gtatctataa gtaatatgag tgatttaaag gataaaaatc ttgttatata
     1381 tccagacgaa aaattaacag cagatgtata tataaaaggt agtttatcaa aggtacaaag
     1441 tgttaaaaaa gaagatatac atgtatatgg aaccattgaa gacccaatac aaggacagaa
     1501 ggaagtatat ttaaaagcaa atattcctca aggtgttact ctagaattta agcatgatgt
     1561 tttagtagta aatcttgaaa aaaacataaa agaaaaaaga gatataaagg tagaagtaag
     1621 tggaaattca aaaagaaata taaataaaat tgaacttagt aaggaatttg tcgatgtatc
     1681 aggtccaaga actctagtta aagaagtcaa aagtgtaaga gctaatctag atgttggtaa
     1741 tgagacagat gatttttcta aaaaattgca attaatacca ttaaatagtg atggaactaa
     1801 ggttgaagga gtaacattaa gttcatcctt tgtgactgca aagatagaac tattgaagga
     1861 aaaaattgtc ccaataagag taagtctaaa tgataataaa gataatgatg tactgaaaaa
     1921 ctataaatta agtaaggaac aagttacaat aaaaggaaag aaagaagata tagacaaaat
     1981 aagttatatt aagacaaaac ctattgactt agctgatttg gctaatggtg aagaaaaaga
     2041 agttagctta gaaattccag agggtataac tcttgaggat agctctataa caattaaatt
     2101 aaatgataca aaacaattga gtgcagaatt tttatataat gcgggagata ttgaattaag
     2161 aaatatccca acaaatatgg ataatccaaa tgtaatttct gatgtagata taaaagttgt
     2221 tgtagagtca acggaggatt tgagtaagtt aaataaaaaa gatatttcat tatatattga
     2281 tttatcacaa ggccctgagg aagatagtaa atataaaata aaatatgaaa caacatatca
     2341 atttaaaaaa ataaatatag aacctaatat tgttgaagtg gaatgaacaa atatttcgaa
     2401 tatttagttg ttacaagatg cgacaaaaaa acctataaaa ttaagtttgt taattttatg
     2461 tttttgttgc atcttgttta ttttagttat attataaaat aaggacaatg agatatttgt
     2521 taaatctaaa aagcgctaga tatatgaaaa aagactgata aatagattta aataaatatt
     2581 ttgaattaaa tatttcaatt atctctagga aaacgttgct gaacgagtta taatttaatt
     2641 gagatataaa aagttctatt tcaaaaaatt caagatgagg tggaaatgca tgagaagttt
     2701 tgaagaagta attaagtttg caaaagaaag aggacctaaa actatatcag tagcatgttg
     2761 ccaagataaa gaagttttaa tggcagttga aatggctaga aaagaaaaaa tagcaaatgc
     2821 cattttagta ggagatatag aaaagactaa agaaattgca aaaagcatag acatggatat
     2881 cgaaaattat gaactgatag atataaaaga tttagcagaa gcatctctaa aatctgttga
     2941 attagtttca caaggaaaag ccgacatggt aatgaaaggc ttagtagaca catcaataat
     3001 actaaaagca gttttaaata aagaagtagg tcttagaact ggaaatgtat taagtcacgt
     3061 agcagtattt gatgtagagg gatatgatag attatttttc gtaactgacg cagctatgaa
     3121 cttagctcct gatacaaata ctaaaaagca aatcatagaa aatgcttgca cagtagcaca
     3181 ttcattagat ataagtgaac caaaagttgc tgcaatatgc gcaaaagaaa aagtaaatcc
     3241 aaaaatgaaa gatacagttg aagctaaaga actagaagaa atgtatgaaa gaggagaaat
     3301 caaaggttgt atggttggtg gaccttttgc aattgataat gcagtatctt tagaagcagc
     3361 taaacataaa ggtataaatc atcctgtagc aggaagagct gatatattat tagccccaga
     3421 tattgaaggt ggtaacatat tatataaagc tttggtattc ttctcaaaat caaaaaatgc
     3481 aggagttata gttggggcta aagcaccaat aatattaact tctagagcag acagtgaaga
     3541 aactaaacta aactcaatag ctttaggtgt tttaatggca gcaaaggcat aattttaagg
     3601 gggaaatagg caaatgagca aaatatttaa aatcttaaca ataaatcctg gttcgacatc
     3661 aactaaaata gctgtatttg ataatgagga tttagtattt gaaaaaactt taagacattc
     3721 ttcagaagaa ataggaaaat atgagaaggt gtctgaccaa tttgaatttc gtaaacaagt
     3781 aatagaagaa gctctaaaag aaggtggagt aaaaacatct gaattagatg ctgtagtagg
     3841 tagaggagga cttcttaaac ctataaaagg tggtacttat tcagtaagtg ctgctatgat
     3901 tgaagattta aaagtgggag ttttaggaga acacgcttca aacctaggtg gaataatagc
     3961 aaaacaaata ggtgaagaag taaatgttcc ttcatacata gtagaccctg ttgttgtaga
     4021 tgaattagaa gatgttgcta gaatttctgg tatgcctgaa ataagtagag caagtgtagt
     4081 acatgcttta aatcaaaagg caatagcaag aagatatgct agagaaataa acaagaaata
     4141 tgaagatata aatcttatag ttgcacacat gggtggagga gtttctgttg gagctcataa
     4201 aaatggtaaa atagtagatg ttgcaaacgc attagatgga gaaggacctt tctctccaga
     4261 aagaagtggt ggactaccag taggtgcatt agtaaaaatg tgctttagtg gaaaatatac
     4321 tcaagatgaa attaaaaaga aaataaaagg taatggcgga ctagttgcat acttaaacac
     4381 taatgatgct agagaagttg aagaaagaat tgaagctggt gatgaaaaag ctaaattagt
     4441 atatgaagct atggcatatc aaatctctaa agaaatagga gctagtgctg cagttcttaa
     4501 gggagatgta aaagcaatat tattaactgg tggaatcgca tattcaaaaa tgtttacaga
     4561 aatgattgca gatagagtta aatttatagc agatgtaaaa gtttatccag gtgaagatga
     4621 aatgattgca ttagctcaag gtggacttag agttttaact ggtgaagaag aggctcaagt
     4681 ttatgataac taatgataaa agaataagaa tcataactgg tcactatgga agtggtaaaa
     4741 gtgaatttgc gatgaactat gttgttaagt taagagatat ggtttctggc aaagttgcta
     4801 tagccgattt agatgtggta aatgtatact ttagaactag agaaaagaaa gagttaatga
     4861 aatccttagg tatacaacca atagatagct caattaatgc tccaacatta gatttaccag
     4921 cagtatcagc agaggtaatg tctcctatgg tagaccattc atacaattct gttatagatt
     4981 taggtggaga taatgtaggt gctagggtta taggaagatt tagtcatttg ttaaaagaag
     5041 gcgattatga tatgcttttt gtaataaatg caaataggga gaaaactcaa acttctgaag
     5101 aagttatcca atatataaaa gaaatagaaa aatcttctaa attaaaggta acaggtctaa
     5161 ttaataatac tcatcttatt aggtttacta ctattgatga tgttttaaga ggtcagaaag
     5221 ttgcaaaaga agtttctgaa aaatgtaata taccgataag atatgttgca tgtttagaaa
     5281 atcttgttga acagttacca aaagatttag aaggagaaat ttttcctata aaattatata
     5341 tgagagaaga ctggatgtag tttgagctca aatatataga aagtttaagg aggcttaggt
     5401 aaatggctaa aggaaaagta tcttttaatc aagaacgttg taaaggttgt ggattgtgtg
     5461 ttgaagcatg tccagtaaaa atcatacaat tagactcaaa tgtaataaac aaaaaagggt
     5521 ataacccagc aactgttttt gaaatggaaa aatgcatagg atgtgcaagt tgtgcaacta
     5581 tgtgtccaga tgtagttata acagtagaaa gagattaatt taattaatat ttaaggggga
     5641 attttaatgg ctaagatact tatgaaaggt aacgaagcat ttggaaaagc agctatagaa
     5701 gctggttgca aatatttctt cggttaccca ataactccac aaagtgaatt accagaatat
     5761 ttatcaagag agttacctaa aataggtgga gcgttcgttc aagctgaatc agaagtttct
     5821 gcgataaaca tggtttatgg tggagcaggt gcaggagcaa gagttatgac ttcttcatct
     5881 tcaccaggag ttgctttaaa gcaagaaggt ataacatatg cagtaggagc agaagttcct
     5941 tgtgttgtac ttaatgtaat gagaggtggt ccaggacttg gaagtataca accttcacaa
     6001 gctgactact ttatgtctac aagaggtgga ggaaatggag actacagaac tccagtattc
     6061 gcaccagcta cagttcaaga agctgttgat atgataatgg aagcttttga cgttgcagat
     6121 tattacagat caccagttat ggtagttgca gatggtatga taggtcaaat gatggagcca
     6181 gttgaattta gagctcctga gaagaaaaga gaattgcctc ctaaagattg ggcaactgtt
     6241 ggaactaaag gaaaaagaaa acctaatgta ataaactctc tatacttaga gccagaagta
     6301 ctagaagacc attgttggca tttacaagaa aaatttgatg caatggaaaa gaatgaagta
     6361 caatacgaaa tgtacaaaac agaagatgct gaatttgtat ttgcagctta tggaactact
     6421 tcaagagtag taaaaagcgc aattgatata ttaagagaag aaggaataaa agcaggtctt
     6481 ataagaccta aagtattatg gccattccca ttcgaagcat tcaaccaaat tcctaatgct
     6541 agaaacatat tgactgttga aatgagtatg ggacaaatgg tggaagatgt taaaatggca
     6601 gtagaaggta aattaccagt ttacttccat ggaagaccag gaggaatgac tccaacacca
     6661 gcagaaatag ttgaaaaagc taaaaaaatt attgccggag aattggttgc aggaggtgct
     6721 agataatggc tgttgtattt aagaaaactg aaggattaca agatactcaa actcactact
     6781 gtccaggatg tacacatggt ataatccata gattagttgg tgaagtatta gaagaactag
     6841 gagtattagg tgatgctgta ggtgttgttc cagttggatg ttcagtttta ggatataaat
     6901 attttaactg tgacactcaa gaagctgctc atggtagagc gccagctgct gctacaggaa
     6961 taaaaagagt tcatcctgaa aatacagtat tcacttatca aggtgatgga gacttagctt
     7021 caattggtac tgctgaaata gttcatgcag ctgctagagg agaaaaaata actactatat
     7081 ttgtaaacaa tacaacttat ggtatgactg gtggacaaat ggctccaact acattagttg
     7141 gacaaagagc tactactgct caatcaggaa gaaatgctga aactcaaggt tatccaataa
     7201 gagtaagtga aatgttagct acattaacag gagctgtatt tgtagaaaga gtagcagttg
     7261 atactccagc tcatgttaga caagctaaaa aagctataaa gaaagcattc caagttcaac
     7321 aagcaggatt aggatttgga atagtagaag tattatctac ttgtcctaca aactggggac
     7381 tagctccaaa cgatgcttta caatggttaa gagacaacat gataccatac tatccattag
     7441 gaaatttcaa aaatgttgaa gttgaggagg tgaagtaaat gtctacagct agagtaatat
     7501 gtgcaggctt cggaggccaa ggtgttatgt ctatgggaca attacttact tatgcaggaa
     7561 tgctagaagg aaaagaagtt tcttggttac catcttatgg accagaaatg cgtggaggta
     7621 cagcaaactg tgcagtaact gtatctaatg agccagttgg ttctccactt ataacagatg
     7681 atgcaactgc agctatatta ttaaatatac cagcatttga aaaattcaaa gatgatgtta
     7741 ttcctggtgg aaaaataata gtaaatagtt ctttaataaa agaaaaagtt gatagaacag
     7801 atgtagatgt ttattatata ccagcaaatg aattagcagc tgaattagga aatgataaag
     7861 ttgctaacat gataatgtta ggcgcttact taaaagtatc tgatactgta gatatagaat
     7921 ctgtactaga agcattcaaa aaagttttcg gacctagaaa agaaaaattc gttccattaa
     7981 acagagaagc tttacaaaaa ggtatggatg ctacatgttc taaagcaact aactaataaa
     8041 gaaaaagatg tcaaaaaaaa gacaatagaa ttatttctat tgtctttttt gctgataagt
     8101 attaaaatat ttttatgact gaaattaagc ctataatgat attttatgaa ctataaaaat
     8161 ataatacaaa ttacaagctt ttcaattatg aaaggagaat ctttagtgag aaagtatttt
     8221 ggaacagatg gagttagagg agtagcaaat acagaactaa catgtgattt agcatataaa
     8281 cttggtagag ctggtggatt tgtcttagct caaggagacc acagagtaaa agtagtagta
     8341 ggtaaggata ctagaatatc aggtgatatg ttggaagcat ctttaatagc aggacttatg
     8401 tctgttggat gtgatgttat tactgttgga ataataccaa cacctgcagt ggcatattta
     8461 actagaaaat atggtgctga ttgtggtgtg gttatatctg catctcacaa tcctgttgaa
     8521 tataatggaa tcaaattctt taataagaat ggatataaat tagatgatga aattgaattg
     8581 aaaatagaag aatatataga cgatatagac aaaattgatt gtcttccaat tggtgaaaat
     8641 gtaggaagaa aattacatga acattgtgca caaagagact atgtagacta cctaaaatca
     8701 ataataagca ctgactttaa gggattaaaa gtagttttgg actgtgctaa tggtgcgtca
     8761 tataaagtgg caccaatagt gtttgatgaa ctaggagcaa gtgtaatttc aataaatagt
     8821 ttaccagatg gaaataatat aaactataaa tgtggctcta cacatccaga acaacttcaa
     8881 agggctgttt tggaacataa tgctgactta ggtcttgcat atgatggaga tgctgataga
     8941 ttaattgcag taaatgaaaa gggtcaaatt gttgatggag accatataat gatattgagt
     9001 gccttaaatt taaagaaaaa taataaattg gcacaagata ctttggtagt tacagttatg
     9061 agtaatatag ggttgacaat agctgctaag gaaaatggaa taaatttatc tactacagca
     9121 gttggagaca gatatgtttt agaagatatg gttaaaaatg gatataatct tggtggagag
     9181 caatctggac atatgatatt tttagattac aacactactg gagatggagt gcttagttct
     9241 ctaatacttg caaatataat tttgcaagaa aagaaacctt tatcagaaat tgcatcaata
     9301 atgagccaat acccacaagt attagtaaat gcaacaataa aaaatgaaaa taagaacaaa
     9361 tatatggaat atccagaaat aaaaactgag atagaaagaa tagaatctat tttagacgga
     9421 aatggtagag ttttgataag accatcagga actgagccat tagttagagt tatgttagag
     9481 ggtaaagagg aaggtcaaat aaaagaatta gcaacagact tagctaatct tatacaagaa
     9541 aaattatcgt aatttaaagg gtattctcag atacaaatac tgaggacacc tttctaagcg
     9601 ccagaactta gttatagaca taattattga gtaatatagg aactaagttg acgaggtcag
     9661 agttaatcga attgtcggcg gatgctctgc ggtgtgttta ccatcgaaag acttctttaa
     9721 ataataggag caatcctatg gacaaaggga agccaaagta aacttatatg acctaactct
     9781 taaaatatat aaataatatt tttaggagga cacaaataat gtgtggaata gttggatatt
     9841 taggaagtag aaaggcagca gaagttatag tagaagggct ttctaagcta gagtacagag
     9901 gttatgactc agcaggagta gcagttaata gttctaatga aaaagaactt aatattagaa
     9961 agtttaaagg tagactttct gtacttgcag aagacttaga aaagaatcct attgatggaa
    10021 atttaggtat agggcatacg agatgggcaa ctcatggaga accatctgat gtaaactcac
    10081 atcctcattt taatcaagcc aaaacaatag cagttgttca taatgggata atagaaaact
    10141 atatggaaat aaaagaagaa ttaatttctg aaggtgttaa gtttgaatct caaacagata
    10201 cagaggtaat agcacattta gtagataaat actatgaggg taatttatta gatgctgtat
    10261 acaaaactat atcaaaatta agaggagctt atgcattagg tgttatatgt aaagaacatg
    10321 gaaatgaatt agttgcagta agaaaagata gtcctcttgt agttggtgtt ggtgaaggtg
    10381 aaaactttat agcatcagat atacctgcat tattaaaata tactagagat gtttatttct
    10441 tagaaaatgg agaagtagtt catctaaaag acgaaaatgt aaccgtttat gactctaata
    10501 gaaatcttgt tgaaaaagaa gtatttcatg taacttggga tgtagaagct gcgtctaaag
    10561 gtggatatga ctactttatg tcaaaagaaa ttcatgagca accaacagga gttagagaaa
    10621 ctttagaaag aagacttgat gataatggaa atataattct agacagtata aatatttcta
    10681 aagaagactt agaaaaaata aataaagttt atatagtggc ttgtggaaca gcatataatg
    10741 ctggattact tggtaagtat gccatagaaa aatttgtaaa tataccagta ataactgata
    10801 tagcatcaga atttagatat agcgacccat ttgtagatga aaattcatta gtaatattag
    10861 ttagtcaatc tggtgagaca gcagatactc tagcagtatt aagagattca aaggctaaag
    10921 gagcaagaat actttcaata actaacgttg taggttcatc tatagcaaga gaatcagatg
    10981 atgtattcta tacttgggca ggacctgaag tagcagttgc atcaactaag gcatatacta
    11041 ctcaaataac ttctttatac atgatagctt tagactttgc tatcaaaaaa ggaacaataa
    11101 ctagagaatt ttatgatagt atgatttcta agatgaaaga aataccatca aaaatacaag
    11161 aaatactaga caatgaagaa tatataaaag aagttgctaa aacagttgtt agttctgaac
    11221 atgcattcta tttaggaaga ggtatagact atagcttagc aatggaaggt tcattgaaat
    11281 taaaagagat ttcatatata catgcagaag cttttgcagc aggagagcta aagcatggaa
    11341 caatagcttt aatagaaaaa ggaactcctg taattgccat agctactcaa gaaaagttat
    11401 ttgaaaaaat ggtttctaat atggaagaag taagagcaag aggagcttat gtagtagcaa
    11461 tagctcaaag ccataataag gatgttgaga aagctgctga taaaataatc tatataccaa
    11521 attcagatga tattttatca ccaatattag cagttgtacc aatgcaatta cttgcttatc
    11581 atgtctctgt actaagagga tgcgatgtag ataagccaag aaatctagct aaatcagtaa
    11641 cagttgaata aaaacagatt aaaaattaaa tagataatat ttattgaaaa gaactcatcc
    11701 tgttatttgt attttatagg atgagttttt tagataaata ttaaatttgt tttgtttttg
    11761 ctataataaa ttttataaaa agattgatat taaatcatct gtaaaatgat aaatatatag
    11821 accataccag gtatagggaa cgataaatat agttcatata aaatatagtg agtacttaat
    11881 aaacatatag tcaatatcag ataaaaaaaa taatagctta tgagaaaaat taaaatacaa
    11941 ggtattagta accttgtgat aagataaagt gagtagttta aaataagaat ataaggaact
    12001 atttataaaa gaatataatg aagctaatag agtataagaa aattacttta ttttagtaaa
    12061 ataatttcaa ataaaaattt ttttaaattg ggagtataaa ttatgacaaa tgattttaaa
    12121 aaagaaaaga aagaagatta ttttgttaat cttaaggcaa tatctactga agttttacaa
    12181 gaaaaagtgc aagataacgc ttgggttata gttgatactc gcttaaatga tgcttataat
    12241 ggctggaagt tagatggagt aagaagaggt ggacatataa aaggagcagt agatttttct
    12301 gctaattggt taagtgtata cttagataga aaagatgaag tgttagaaca agcattgaaa
    12361 actaaaagaa ttgatttaga taaaaatata gtattgtatg atgcaaatgg aaaagatgct
    12421 ttggttgtag cagattattt atctaaaaaa ggatataaat atctatataa atatgatatt
    12481 aaacaatggg cagacgacga aaatcttcca atggaaagat ataaaaatta tcaaatgata
    12541 gttcctgctt ttataataaa agatatatta gatggaaaaa ttcctgagac atttgaagat
    12601 tcaaagaata taaaaatgat tgaagcgagt tggggagaag aatcgtatac aaaagggcat
    12661 atacctacaa gtgttcatgt aaatactgat attatagaac ctcctccaac atggatgctt
    12721 gataatgatg ataacttaac taagtttgct ttagactatg gattaacaaa ggatgatact
    12781 gtaatagttt caagtagtac acctatggca tcttatagac tagctgttat attgagatat
    12841 ataggagtaa aagatgtaag agttttgaat ggtggaacaa actcatggtt atcagcagga
    12901 tatgaattag aatttattag taatccaaaa cattcatgta ctaattttgg agcagatata
    12961 ccagttaact cacaattagt tgttactaca tctgaattaa gacaaaaact aaaagaaaaa
    13021 aataagttta tactagtaga taaccgtact tgggatgaac atataggaaa agtatctgga
    13081 tatacatatt atgataaaaa agggcgtata ccaggagctt tatatggaca ttcaggtagt
    13141 gatagtgttt cactagaaga atatcgtaat attgataata caatgagaaa taaatatgag
    13201 attttagaaa tgtgggacaa ggaaaatata gatgtgaata aacagcttat atttatgtgt
    13261 ggaagtggat ggcgtgctgc tgaagtatta acatatgcaa atgtcatagg agtggaaaat
    13321 acctcccttt atagtgatgg atggatgggc tggagtcttg ataattcaaa tttaattgaa
    13381 gtgggagaac acaaataaag tttttgaaag tacaatagag ttgtttaaaa caaaggtgtt
    13441 tttgatttat taggaagagt gtgtaaattg tgtattggga agtgtactta ggtatgttca
    13501 tttggagcta tggaaattga taaaaaagta ttatctgttt attttgttgg taaaatatga
    13561 atagattata gattatagat tatagattat agattataga tttaagtata gactaagtaa
    13621 tttatattac agaagagaat atataagaat taatgcaaga tgtatttgat acatataaga
    13681 aattggcaag tccaggagaa ggatagagaa aattgagatt gaagagtttg aaagagatta
    13741 ttagatgaaa aatacaaaaa tgtataaaaa tacatattga gaaagtatat ctctcttaaa
    13801 tttgttaata attctattat acagaaaaac atatttaagg gggatttttt actatgaaaa
    13861 aagtaaaatt agctataagc ttttttatat taatgattat aggaatgaca atatcttatg
    13921 cagatacaag tataaatact agatacaata gattaagtaa agctcttgaa atatcagaag
    13981 caaattttaa attttataat gttaaaatta attctgttat aaatcataat ttatcaaata
    14041 aagaatttca taatattttt atagaaataa ttaacaatct aaagttggat aaaaaaggaa
    14101 taaattggaa tcaaaaaggg tctggcgata aaatacaatt atgtgctact ataaaaggta
    14161 gcgataaaga aatttctata aaaggaataa ataaaaataa caaagaaagt tacatagtag
    14221 ttgacatatt ggataacaaa gtatataaac ataaagaggt tatttatagt aacgtagaaa
    14281 atgtgttaaa taaatattca tctcaagtag atgtgtctgc ttgtattgtt ggagaataca
    14341 caaaaaggtt acaaaacaac aaatatgatg atattttaca aaaaatatta tataatatga
    14401 gtgctaagca aatagatgaa gttaaagacg taaacttttt atctataaca gcatatagta
    14461 aagtattaaa tgattatgaa ttagaatatt taggggaaaa aattaattta aatataggaa
    14521 ttaaatataa tgaggatgac ggaaaaacac tcatatatat tgccacacca attataaaat
    14581 tagattatta attatgagga gatcaaaaaa tatggctaag ataatagtaa aaaagagtaa
    14641 tccacttaaa ggtagtgtta aaatagatgg tgcaaaaaat gcagttttac caataatagc
    14701 agcgacttta ttagcaaacg gaaaatcaac attaaatgga gtaccaaatt taagagatgt
    14761 tcatgttata tcagatttat taagacatgt aggagcagaa gttgaatata aagaaaatac
    14821 acttactgtt gatgcaagca atataaaaac ttgtgaagct ccatatgagc ttgtaagaaa
    14881 gatgagagct tcttttttgg ttatgggacc attgcttgct agatttaata gtacgaaaat
    14941 ttctatgcca ggaggatgtg ctataggaac aagacctata gatttacatt taaaaggatt
    15001 taaagcctta ggtgcaaaga tagaaatgga ccatggtttt gttgaagcag caacagaaaa
    15061 gttagtagga aataaattat atttagattt cccatctgtt ggagcaactg aaaacataat
    15121 gatggcagct tctttagcag aagggactac aataatagag aatgcagcag aagagccaga
    15181 aatagtagat ttagcaaact tcttgaacga aatgggagca gatgttaaag gtgcaggtac
    15241 taatactata aaaataaaag gtgttaaaga gttaaaagga gcagaacata atgtaattcc
    15301 agacagaata gaagcagcaa cttatatggt tgcagcagct atgacaaaag gtgatataac
    15361 tgttgaaaat gttttaatgg agcatttaaa accagttgta gctaaattaa gagaagctgg
    15421 ttgtgaaata acagaaatgg acaattctgt aagagttgta ggtccaaaag tattaaaacc
    15481 aatagatata aaaactttac cacatccagg atttcctaca gatgttcaag cacaatttat
    15541 ggctatgctt actgtagcca atggaactgg tgtagttata gaaactgttt ttgaaaatag
    15601 atttatgcat gttgctgaat ttaatagaat gggtgctaat ataaaaatag atggaagaag
    15661 tgcggttgtt aatggtgtag atgagctaca cggtgcagca gtaaacgcta ctgatttaag
    15721 agcaggggca gcccttattt tatgcggtct tatagcagaa ggagaaactc aaataggaga
    15781 aatataccat atccaaagag gttatgttga tatagataag aagataacag cactaggtgg
    15841 tcaaatcgaa atagtagaag attaataatt ggtttataga agaaagttat aaattaatag
    15901 ggttgttcat atacttaaat acaaggtttt aaaagctaag gaggagagta tatgaagaac
    15961 ccattggttg ttttgttagg atttgtgact tgctctgtgt tagttccatc acttataact
    16021 ttagtttctt ataaaaatgt agaattaact gaaaaaccag agagcccagt atctataaac
    16081 aaaactatta aaaaaagtga tattgaagac aaaggtaata aagaagaaaa aaatacagtg
    16141 aactatgaaa ctgtaaataa aaaagcacca attatcaatg tatataatca tataacaggg
    16201 aaaaccgaaa agatggacat ggagaactat ttatgtggag tactagctgg tgagatgtct
    16261 tcagagtttg atatagaagc tttaaaagct caatctgtag cagctagaac atatgtagta
    16321 tataaacagg aacatggtaa atctagtaaa cataaaaatg cagtagtatg tactgattat
    16381 aaacattgtc aagaatataa gagttatgat actcttaaaa aattaaatgg tgaagaatgg
    16441 attaagaata aatattccaa aatccaagaa gcagttagag gaactaaagg tcaaataata
    16501 acttataatg ataaggcgat acttcctctt tatttttcta catcttcagg aaaaacagaa
    16561 aatagtgaag aagtattttc tgcaaaatat ccttatttaa agtctgtgga aagtccttat
    16621 gataagtatt cccctaaatt tgcgtcaaca cttaaaataa gtaacacaga ttttgttaaa
    16681 agtcttagaa gggcatatag cactattgtg atagatgtaa ataatttaag taaacaagtt
    16741 tcaataacta aaagaagtga tgccggaact gtagaaaaaa taaaattagg aaataaagaa
    16801 ttgactggaa aagacattag aactgttttt aaattgaact ctgcaaactt tgacataaaa
    16861 tttggagaag gatacataga tttcgtggta aaaggatatg gtcatggtgt tggtatgagc
    16921 cagtggggag cagaaggaat ggcagaagag ggctataaat attatgatat attatcacat
    16981 tactacacag atacaaaaat aaaagatata tactaataat tgtataaaat gccaaaaaca
    17041 cgtcaataat ccaaatgtag aaatatattt ggaggtgcaa aatgaagaaa aagctgttag
    17101 aaaaagatgg tttttattta tctttgtttg tatgtgtttg tttattagca gtaggtggag
    17161 tttggtttac aaataataat gtagataaat tggcttctaa taaaggaatt atggaaaatg
    17221 ctaataaaga tagtgaagag gaaatacatt taattgaaaa agacaagaaa gatgctatcc
    17281 ctactgcaac ggattcaaaa caaaacttag aaaaagctaa gtcaaaagag gaaaataaat
    17341 cgagtacaac aaaattaaat tacattggag ataaagtaat aagaggatat tcagaaaaag
    17401 agcctagtta ttctaaaaca cttgatgttt gggaaactca taaaggtgta gatattagtt
    17461 gtactaaagg caaagaagta aaatctctat taaacggaat agtagtagat gtatttgatg
    17521 atgaagaata tggacaatca gtcaaaataa aaagtgacaa taacattgta gtagtatatt
    17581 caaatctaga taaaaatgtt agtgttaaga aagaacagaa ggtaacagaa ggacaatctc
    17641 taggtactgt aggtagtact tctcaaatag aaagtgaaga aggtatacat gtgcatttag
    17701 aggcgtatag tggagaaaaa tctatagacc caatgagtct aattaagtaa tagtataaca
    17761 agtttaatca tatttggtta agcttgttat attttttggt gtttttacat atatatcatt
    17821 aaagctatta tgtgaggggg gtaatctttg agatctcata tagaggaaag ggccatagtg
    17881 gtagcaaaat atatacttga aaaaaacact acagtaagac aaaccgccaa gacatttgga
    17941 gtaagtaaaa gtacaattca taaggatgtt acagaaagat tgaaggaaat aaatccatcc
    18001 cttgccaaag aagttaaaaa tgtgctggac aaaaataaat cagagagaca tattagaggg
    18061 ggattagcaa cgaaattaaa atatgagaag gaaaataaaa aaatgtaggt taaaaaccta
    18121 ctttttttat tgaattaaat tataaaaaga aatatattaa gtaattataa atagcaaaat
    18181 aataatactc tcaaaagatt tattttctct tgattaaagc gagatttttg tatatgcttt
    18241 attataggag tattaaaata ggcttataat ataatctaat attataaata aaggagttga
    18301 aaaaatggct ggagctgata taggcataga tttaggtact gcaaatgtgc ttgtttatgt
    18361 tagtggtaag ggaatcgttt tagaagaacc atctgttgtg gcgatagaca aaagaacaga
    18421 ttctgtttta gctgtgggag aagaagctaa aaagatgata gggagaacac caggtaatat
    18481 agtcgctata agacctttaa gagatggtgt aatttcagat tatgatgtaa ctgagaagat
    18541 gttaaaatct tttatagata agatagttga taaaaaggga tttggaagat tttttatgcc
    18601 aagaattatg gtatgtgtac caacaggtgt cacagaagtg gaaaaaagag cagtagaaga
    18661 tgctacgaga caagctggag ctagagaagt atatatcata gaagaaccaa tagcagctgc
    18721 cataggagct ggtgtagaca tatcacagcc taatggtaat atgattatag atattggtgg
    18781 aggtactgta gatatagctg taatatctct aggaggagct gttgtaagtg aatctataaa
    18841 ggttggtgga gatagatttg atgatgctat agttaaatat atgaaaaaac aacacaatct
    18901 tcttatagga gaaagaacag cagagaaaat caagtttgaa ataggttctg catttaagag
    18961 agaagaagaa aaatatatga agataacagg aagaaattta attacaggac ttcctagttc
    19021 tataactata aattcaacag aaatgttgga agcacttaga gaatgtgttg agcaaatagt
    19081 agttgctact catgctgttt tggaaaaaac gcctcctgag ttagcagcag atataggaga
    19141 tgtcggtata ataatgactg gaggaggttc attattatat ggattagata aaataataga
    19201 acagaggaca ggaataactg ttacaatagc agatgaacca ttatcttgtg ttgcaaaagg
    19261 tacaggaata gctttaggtt caatagactt acttgaaact ggtgggtctt tcaaaagaaa
    19321 ataattagga gagtttagaa tgttaaatat agatgaaata aaaaaattaa taccacatag
    19381 atatccattt ttattagtag acaagattac agagctagaa gttgggaaaa gagctgttgg
    19441 tataaagaat gtaacagtta atgagccatt tttccaaggt cactttccag aatatccttt
    19501 gatgccagga gtactaatag tagaagctct agcacaagta tgtggagtgg caatgatgtc
    19561 tgtagaggaa aacaaaggaa aactaggtgt atttgcaggt atagataaag ttagaatcaa
    19621 gagagaagta agaccaggag atacattaac tatggaagtt gaaatgacaa ctcttagaaa
    19681 aaatatagct aaggcagatg ctaaggcata tgtaggtgaa gaacttgtct gtaaaggtga
    19741 attaatgttt gctttagtag aaaaataaaa acttgaaata taaaagtaaa aaggtagata
    19801 atttggagta tttaatcaga tactctaaaa tatctacctt tttatataaa gaattttatc
    19861 atttagttta tatttagatt aggtggatac taataaagcc aaggctattg tttcagctaa
    19921 atctaatata aagctaaggc gtatattatt aaatgaaagt ttattatttt catcaatttt
    19981 atctacaatt cctactatag aataatttcc tatttgaggt agttttttac caacaccttt
    20041 tccgggcagg ataggtcctt ctctaatctg tatattacct atgttactct gcgaccccaa
    20101 gcaagcatct atagccaaga agttaccttg tatatgtgta ttttttatag tatctaaaga
    20161 ctcatatata tttaaggcgt gaatagggtt atctagtgta ccgtaaacgg ggtatttaaa
    20221 gttactgttc ttaagtattg ttccaactaa agggcctaaa gtatcaccta tagccctgtc
    20281 tgttcctatg caaacaacaa tcgtattttc attaattata gtttttagaa ctgaacttag
    20341 catctgtata acatcatcat cgctgtagtt tactttagct aaatacaata ggaacacccc
    20401 cttcaaaaaa atatatgaat aaaaagcatt aaaaagacct aactttaaat ttcagataga
    20461 tagaaaattt tatttgtctt acaaaaaaag gaatagaaaa aatatgacta aaaatattga
    20521 caaaatttta aaaaaatagt aagctagtaa agtaaaacaa aacttaaatc ttgattacct
    20581 tatcaagaga ggctgaggga taggccctgt gaagcccagc aaccacccaa atgggaaaag
    20641 gtgctaattc ctacaagaca aaagtcttga aagatgaggt cagttaatta gtatgtaatt
    20701 acgattatta acctcttctt ataaaggaga ggttttttta ttttgagtaa atttctaaaa
    20761 ttaaaaataa ggtaaaacct ataataaaaa ttaagaataa ataaggggga ataaaaatgg
    20821 caagacattt atttacgtca gaatcagtta cagaaggaca tcctgataag atatgtgacc
    20881 aaatatcaga ttcaatatta gatgcattat tagaaaaaga cccacaatct agagtagctt
    20941 gtgaaactac agttactact ggtttagttt tagtagcagg agaaataagt acatctgctt
    21001 atgtagacat tcctaaatta gtaagagaaa cagtaagaga aattggatat acaagagcaa
    21061 aatatggatt tgattgtgat acttgcgcag ttataacttc tattgatgaa caatctggag
    21121 atatagctat gggagttgat gaaggtctag aaagtaagac tggagaagag atagaagaag
    21181 aaatagaaaa agttggagct ggagaccaag gtataatgtt tggatttgca tgtaatgaga
    21241 ctccagaatt aatgccactt ccgatatctt tggcacataa attatcaaga agacttacag
    21301 aagttagaaa aacaggttta gttgattatt taagaccaga tggaaaaact caagttactg
    21361 ttgaatatga aggaagtaaa gctgtaagag tacatacagt tcttatatca gctcaacatt
    21421 gtgaaacagt atcaaatgat aaaataagag aagatttaat taatcatgtt attaaggaag
    21481 ttataccagc agaattactt gatgaagaga ctaaaattta tataaatcca acaggaagat
    21541 tcgttatagg aggaccacaa ggagatacag gtcttacagg aagaaaaata ataatagata
    21601 cttatggtgg atattctaga catggtggag gagctttctc aggaaaagac cctacaaaag
    21661 ttgatagatc tgctgcttat gcagctagat atgttgcaaa aaatattgtt gcggctggtc
    21721 ttgcagacaa atgtgagata gaacttgcat atgctatagg tatagctaga ccattatcta
    21781 tatttataga tacttttggg acaggcaagg tttcagaaga aaaacttgtt gaattagtaa
    21841 ataaacactt tgatttaaga ccgggagcga taataagaga cttagattta agaaagcctc
    21901 tttataaaaa ggttgctgct tatggtcact ttggtagaac tgatatagat ttaccttggg
    21961 aaagaactga caaggttgag cagttaagaa aagatgcttt aggtgaataa tttttaacta
    22021 aaaagctgtt agaatgtaat tttatgatta ctttctaaca gcttttttat tatgagttaa
    22081 atatttttaa taaatgttaa tttatcatca tataatgaat aaacagtatt taatctggtc
    22141 aatacatttt atataatact ctactaaatt tatacatatt atgggatata tgttaaagga
    22201 tggttaagaa tgtgttaact aatgttaaat aattaaaaat atattgaaat atcacaataa
    22261 tgagtctaat ataatcttaa taaacttaca ctttaaaaat aattttaatt gggggggagt
    22321 agtgtatgtc aggcaaagta aatattaaaa cggttataag ttttgctgga gcttatgttg
    22381 ctactgtaat aggttctgga tttgcaactg gtcaagaaat actgcaattt tttacgtttt
    22441 atggatatgc gggtattata ggtggaataa tctctatggt actattttca tggtttggag
    22501 cagaagttat agataaggga agagaattga aacttaagga gccaataaaa atttatcaag
    22561 tatattgtgg aaaatatctt ggaacatttt ttgaatggtt tggtccattg tttttatttg
    22621 gaacattagt aataatgatt gcaggagcag gagctacatt atcagagtac tatggattaa
    22681 atccatatgt aggtaggatt ggtatggcaa tcgtttccct aataactgtt tcattaggtt
    22741 tgacaaggct ttctaaaata ctaggtaata taggacctat aataataata tttacacttt
    22801 tagtaggagc aattagttta tttagcaata ttgatgcttt atcaaatgct ggaaatatgg
    22861 ttgatagttt aaatattaaa actgctacat caaatggata tttctcggga gtactttata
    22921 cttgttacaa tgtaataata gtaataacat tcttgacagg aatgggagct tctgcagtaa
    22981 ataagaaaga tgctgtatgg ggtggtatag taggtggagt agcacttatg gcagcagcta
    23041 taatgatgaa tttggcatta ctttcagaca taggaaatat atacacaaaa gagataccag
    23101 cactttactt agctgataag atatctccaa taataggtat tttattctca gtagtacttt
    23161 tactaggaat atacactaca gctgtaccat tgttatggtc agtaacaaac cgatttgtag
    23221 aagatgacca ccctaagttt aaaattataa caatagtggt aagtatatta gcttgtatag
    23281 gaggtttact gccatttgat aagttagtag gtacactata tccatataca ggttacatgg
    23341 gaattttaat tttactatgt atgctttata gaagaattac taaaactgag gggtacaagg
    23401 agaataaatc tgaaataagt tagtactgta atatacttat gaaatttaat ttatactcta
    23461 aatatagagt aattaaaata aaatagctat ttaaatatgg tacctgtatt tcatggtacc
    23521 atattttatg gatgcagatt ttttttgttt ctgataaaat gtataatagt tggaaattac
    23581 gttaagtata aaaatataaa acttttctac gattatcata gttttaatat ataaaattat
    23641 actaacattt aaatggagcg attattagta tttttaggag ataaaaatgg aaaaattaga
    23701 aggaatgata agtgaaatag tctttaaaaa cgaagataat ggttatacaa tagctcacct
    23761 ggtaaatgaa aatgatgaaa ttgtggttgt aggatgtatg cctactttgg caattggaga
    23821 aagcatagaa gttgaaggaa agtgggtaaa tcataagatt tatgggactc aatttgaagt
    23881 gaatagtttt atgccagtaa caccatcatc cttagaagga atttatgttt acttatcttc
    23941 tggaatgata catggtattg gtgaaaaaat ggcaaaaaga ataattgata cgtttggtgt
    24001 agatacgtta gaggtgatac aaaattcacc agaaaaacta caagaagtag aaggtatagg
    24061 gagtaaaaaa gtaaaacaaa tagtaaaaag ttatgaagaa gacagagaat tgagaaatat
    24121 aataatacag ctatctcctt ttggaataac accaaattac tgcctaaaaa tatataaaaa
    24181 atataaaagc agtgcaatag aagttataaa taaaaaccca taccaacttg ctgaagatat
    24241 aagaggaatt ggatttaaag tagctgatag tatagcaagt aaaattggta tagataaaaa
    24301 ctctaaagat agaatttgtc agggaatctt atacacttta aataaatctt taagtaatgg
    24361 gcatacatat ttaccagagc atgttctaat acaagattcg gaaaaattgt tggagctaaa
    24421 tggagaaata ctaaaagagt gtgtaatgat gctagtttat aatcaaaaaa tacatataga
    24481 gaaagtaaac aatgaaaatc ttatatacct tatgccatac tatttagctg aaaatggagt
    24541 atgtagtcaa atagtaaaat tatcacagta tgaatttgaa gacttaaaaa tagatataga
    24601 taacgagatt aatgttttag aagaagataa gaaaattaag ttagcagaaa aacaaatttt
    24661 agcagtcaaa gaatctgtta atagtggagt attaattata actggtggac caggaactgg
    24721 taaaacaact accattaatg ctatcataga tatatttgaa aataatggta aaagtgtgac
    24781 tttggcagct ccaacgggaa gagcagcaaa gagaatgagt gaaacttcta ataaagaagc
    24841 aaaaactata cataggttgc tagaaatggg attttcaaca gatgatgatt taactttttt
    24901 taaggatgaa gaagacccaa taaattcaga tgtaataatt gttgatgagg tatcaatggt
    24961 agacattatc ttaatgtaca atttacttag agctataaag ttaggaacaa gagtaatttt
    25021 agttggagat agtgaccaat taccttctgt aggtgctgga aatgtactta aggatatgat
    25081 taattctaat attataaatg tagttaaatt aaatgaaata tttagacaag cgcaagaaag
    25141 tatgataatt gtaaatgccc ataagataaa caatggagaa ccactttatc taaatacaaa
    25201 aggtaaagat ttctttttta taagaaaatc aactaatgaa gaaattttaa atgaaataat
    25261 aggattggtt aatgaaagat tacctaaatt ttataaagtt gagaaattaa aagatataca
    25321 agttttatct tcaatgagaa aaggtgaatt gggagttact aatctcaata tagagttaca
    25381 gaagtatctt aacaaaaaag aaaaatttaa agttgaagaa agtttttcta aaagattatt
    25441 tagagttggc gataaggtaa tgcaagtaaa aaataattat acaaaaaaat gggaaacaga
    25501 agaccaaaaa gaaagtggag aaggtatcta taatggagat ataggatatg tttatcacat
    25561 agataaagat aagaaaacta tttatatatt atttgaccag actaaaattg tatcatacct
    25621 ctatgatgaa ttagatgaaa ttgaccatag tttttgtaca acaatacata aaagccaggg
    25681 aagtgaattt ccagttgtag tattgccaat agcgtgggct cctcctatgt tacttagccg
    25741 taatttatta tatacagcag taacaagagc aaaaaaatta gttgtattag ttggagatgt
    25801 gaaatatctt gaatatatga taaaaaataa tagagttaat caaaggtatt caaatttagg
    25861 atataagcta aataaattta aacaagaagg gttgttgata gaataaatat gaaaatatgc
    25921 aatgataatt ttattaagaa ttttaaaaat atgattaata aatgtttgga ttttatatat
    25981 cctgagaata taagttgcat aatttgtgat aaatccataa agaaaacaaa tacttattca
    26041 atatgtaaaa gttgttttaa agaaatgaat tttatacaag atggttgcat gaagtgtgga
    26101 aaaccaataa tacgtcattc aattgaaaaa gaatttattg aagagtgtag ttattgtttt
    26161 aataaagact tttattttga taaatcaata tcttgtattg aatacaatga tgtgacaaaa
    26221 aagatggtgt tagggctgaa gtataaccaa aaaaccttta tggctaagta tatagctcaa
    26281 ataatgaaag aaaaattata tttagaaaac ataaaatttg attatatatt atttgttcca
    26341 cttcataaaa aaaggctaaa taaaagaggt tttaatcagg cacaaaaaat agcatttaat
    26401 ttaagtaaaa tagtaaatat tccattatta gattgcataa gtagaaaaaa atatacaaga
    26461 atgctatata aattgaataa gaaagaacga aaagaagaat taaaaaatgt atttgttgtt
    26521 aaagaaaatg ttaaattaat aaataataaa aatatacttt taatagatga tatatttact
    26581 actggtctaa caacaaacga aatttctaaa cttttaaagt tatcaggtgc taataaggtt
    26641 tttgtattga ctctacttac aaaagcaaat gataattata ttatggaata atcttatata
    26701 ttgacaattt tagtttaaaa tattaaaatg aataagtgat atttaggtct gtggttgaaa
    26761 gtccaagcca gtcgcaggca aagggatcca cgtaagttaa cactaaaaat tgggttaatg
    26821 atcatggtgc ggcttagaag taagacctac cgataataaa atcgagagag agtagtagtg
    26881 cggcgaaccc aagcgaaccc tccagtaggc gagtgtgggg tcaaaaacca ggtcagctaa
    26941 atatcatatt aaaagagagc tagcatacct ttatgtatag ctctttttta tatataataa
    27001 atttaggttg agtcataaag aggtgatatg tttgaataaa agacaagaaa atttattaac
    27061 aacattgcta ttaaaagaag agtttataaa tgttaataag attgccaatg attttgagtg
    27121 ttcagaaagg acaataagaa atgattgcaa atatattgat gaatggttat ctactttttg
    27181 taatgcttat atcaaaagaa aacctaatat tgggattaaa tttagtggag atggaacaga
    27241 caaaatactg gtaaatcaga agttgcgagg tgaagttaaa agagtaaata gtgagcttca
    27301 taagcaagtt gagatattaa atttactact ttgtaataat aaaaaagtta ctttaaatga
    27361 tttatctaat aagttgtaca taaataaaaa tattgtcaga gaagaaataa gtaaattatc
    27421 agtagcatta aataattata atttaaatat ctcgactaaa aaaggcagtg gtatatatat
    27481 tgaaggggat gagaaagaca taagaataat gttagtatca ttcttattta aatatataga
    27541 taaatacaga ttaaacatag atgaaataga gtacttccat atagtagata taataattgt
    27601 aaaaaacata atgcttacta tagaggagga tatggatata agacttacag atatatcatt
    27661 caagcaattg atgctatttt tgcttataaa tataatacga ataaggctgg gaaattcttt
    27721 aaaagtatca aatactaaga aagagcttaa gctaaaaatg cttatattgg atatagaaga
    27781 tgaattgaaa tctaatttat ccatagtatt aaattatgaa gaaaaattgt ttatagaatc
    27841 tttaatattg ggtatgaaca aacaaataaa tagtaataat attaagaaat ctttatatct
    27901 aaatgaagaa ttagcaagtt atacaaaaaa aattataaat ttagtatcag aagaatcagg
    27961 aataaatttt aataatgata tattacttta tgaacaatta atatgtcatt tgaatgttac
    28021 aatgcatcaa atgaaaagca atgtttactt agaaaatcca cttttagata acataaaaac
    28081 taaattttgt tttttattta cagtaatatc aagttcaata gaaattgaat taaaaggaaa
    28141 acaaataacg gaagatgaaa ttggttatct tacacttcac tttcaaacct ctctagaaag
    28201 aaaatacagc aaaagggaat caaataaaaa ggcaagtatt gtatgtccat ttagttttgg
    28261 agtaagtatg ctattaaaag taaagataga aaagagattt gataacataa aaattattga
    28321 aacattaaga gaggaagatt taaaaagaga taactttaat aagactatag attttataat
    28381 tgcatttcaa gagtatgaaa atatccaaaa accaattttt ataacaacac cattatttac
    28441 tgatgaggat gagaataaac ttaaagagtt cacaaacaaa ataaaagaaa aagacacttc
    28501 atataaactt atgaacagat taatgatgtc tgattattta ataagagagt tagaacatga
    28561 tgatatttat gaagctataa catatttaac taatttatta attgaaaaac attatgcaga
    28621 aaaagagtat ttacaatcta ttataacaag agaaaagtca tatccaacca atgttggaaa
    28681 aggaatatta ttccctcatg gagacatgaa atatataaaa caatctgttt tatgttttgc
    28741 aaggttaaaa acaccaataa aaattagaaa tggaaaggat ataaagatta tacttttgtt
    28801 agcatataaa aaagatgatg atagaaaagt atttagagaa ttatttaaag aaatatctaa
    28861 tttaacagag gatgaaaata tgctagatat tctaagtaaa tgtgacattg aaaaggtaaa
    28921 agatattctt atataaaatt catagtgaac tatggataag cacaaaattt tccatgtgat
    28981 ggaaaaagtt gtgcatgttt ttttgttttt ataagatatt atatgtttgt aaggtataaa
    29041 taatctaaaa gtagagaagc aacttaggag gaaaaaatgg aagaaatagt tttaaagatt
    29101 ataattcatg ctggaaatgc aaaatctatg ctttatgagg cacttgacta tgcaaaggaa
    29161 aacgatttta aaaaagcaga tgaattaatt gaaaatgcaa atgaagaaat tttaaaagca
    29221 cataaagtac aaactgaact tatacaaaag gaagctgggg gagataaatc agatatatct
    29281 attttattga ttcactctca agaccattta atgacttgta tgagtgagag aaaccttata
    29341 aaagaaatga ttatgttaag aaaagaaatt caaaaactaa aataaaaaat taagggggat
    29401 ttaaaaaatg aaaagaaaag tatatttatt ttgtagcttt ggtatgtcaa caagtttact
    29461 tgcagataaa atgcagaagg tagcagatga acacaattta ccaatagaag tagaggcatt
    29521 tccgatagca gaaattgaca aaatagttga agaaaaaaat ccagattgta tattattagg
    29581 accacaagtt aaatacatgc taaaggagtt aaaaccaaag tttgaagctc aaggaaaact
    29641 tatagatata atcaatgaag ttgattatgg aactatgaat ggggaaaaag tattaaaact
    29701 tgctataaag ctaataaaat ctaaacaagc gtagaattat agtaaaaaat aataggaaat
    29761 gagggtggat agatgaatag attggaaaga atacttatgc cattggctga aaaaataggt
    29821 aaaaataaat tattaattgc aattagagat ggttttttag tatcatcacc attattaata
    29881 ataggttcat tatttttatt agtagcaaat tttcctataa aaggatggaa tgaatttgta
    29941 ggtcaaattt ttggtccaga ttgggctgta aaattgcaac aaccaactgt agcaacattt
    30001 gaagtaatga ctctacttgc agttttaggg attggatatt cttatgcaaa gcaaatagat
    30061 gtagacccaa tagcatcagc agcagttgca atagttgcct tttttataat aacaccattt
    30121 gtaataccat atacaccaga aggaacagaa tcagtttaca tggtaacagg tataccacta
    30181 ggttggatgg gttctaaggg aatgtttgta ggtatgataa cagcaataac ctctgtaaaa
    30241 ttatttgctg cagttgtaaa aaaaggatgg acattgaaaa tgccagatgg agttcctcca
    30301 actgtatcaa aatcatttgc agcattaata ccaagtgcaa tagttatggt agttttcttt
    30361 ttagttaaga tagcttttga agctacacca tatggaagta tacatgaatt tatatttaag
    30421 tttttacaaa tgccattatt aaaattagga aatagtttag gtgctatggc aatcgcttat
    30481 atattcttac atttattctg gttctttgga ataaatggaa gtagtgttgt tggggctgtg
    30541 tataatccaa tattaaaaat tctatcagct gaaaacttag ctgcttttca agctggttca
    30601 aaaataccaa acataataac aggtcaattc caagatatgt ttgcgacatt tggtggagca
    30661 ggtagtacac tatcattagt catagctatg ttattaatat gtaaaagtaa acgtataaag
    30721 tcaatgggaa aactatcaat tttaccagga atatttggta taaatgagcc attgatattt
    30781 ggtctaccaa taatgcttaa cccaatacta ttgataccat ttgcaatagt tccaacaata
    30841 aatataataa tagcttactt ctgtatgtca gcaggattag tgcctcttac aaatggagtt
    30901 cagttaccgt ggacaactcc aattatattc tctggttttt taacaacagg atggcaaggt
    30961 gcagtcttac aattagtact tcttatacta ggaatattta tgtatatacc atttataaaa
    31021 atgctagata agcaatactt gagagaagaa aatgaaactg ttgaagagga agatgatgat
    31081 atatcttttg atgatttagt attataggag gaagattatg aagaaaatag ttatgatatt
    31141 ggaccaaata caagctggag ctggaggaaa agagaaaagt aatatacctc ctgctggaaa
    31201 atcaagccca ttaggaccag gtgtaatgat ggagcaattt ttaaatgaat ctaaggtaat
    31261 agctacctta ttttgtgggg atgaattttt tgtaaataat caagaagaag taacgagtaa
    31321 gatgatagct atggtaaaaa aattaaaccc tgatgtagta atctgtggac caagctttaa
    31381 ttacgaaaac ttttctaaga tgtcagcaat actgtctaaa aatataaatg ataaaacaga
    31441 tataccagct tttgcagcta tgtcagaaga aaatatagat gtgataaacg aatataaaaa
    31501 tgacatatgt atagttaaga ctccaaaaaa aggtggaatt ggtcttaatg attcgttaaa
    31561 taacatatgt aaattggcaa aagcaatagc aaataaagaa gatataactt tgatgaaaga
    31621 agaattttgt tattaataag agtattaatt attagcaagt ttaaatatta tactatttat
    31681 aataagcata cattagtaaa atctatataa tttaaggagg ggaattttaa catgtgggga
    31741 atgatagcga catggagaat ggccctagaa ggaataactg aggcatcaga acatttagaa
    31801 aaaggcatgg aagcaggtga tgctgtagag cttgctgtaa gaagagttga agactatcca
    31861 ttctataaat cggttggata tggtggactt ccaaatgaaa attgtgaggt agaacttgac
    31921 gctgcattta tggatggtaa cacattagat attggcgcag ttgctggtat aagagattat
    31981 tctaatcctg tcagcatagc taggaagtta agttatgaga aggtaaataa ttttttagtt
    32041 ggaataggtg ctgaagatta tgctcataaa aatggatttg aaagaaaaaa tatgttgact
    32101 gatagagcga agttacatta taaaaagaga aaaaaagaga ctttagacaa aggattaagc
    32161 ccatatgctg gtcatgatac tgttggtatg atatctttag acaaaactgg aaaaatgtgt
    32221 gcagctacat caacaagtgg attgtttatg aagaaaagag gtagaatagg agattctgca
    32281 ctatctggtt caggattcta tgttgatagt agtataggag gagctacagc tactggtttg
    32341 ggagaagatt taatgaaggg atgtatatct tatgagatag ttagacttat gaaagaaggg
    32401 aagcatccac aagaagcatg tgatattgca gtaaatgaac ttgataaaca gcttatcaaa
    32461 agaagaggaa aagctggtga tttatcatta gttgccatga ataataaagg tgaatgggga
    32521 gtagctacaa atatagctga ttttactttt tcagtagtaa ctaaagaaga atctcctact
    32581 gtatatattt caaatagagt agatggaaag actatacata aggttatatc aaatgagtgg
    32641 atggaagcat atgaggctag aataaaagca ccaatagaag taagtgagta aagtagtggt
    32701 tacataatta aaatagagtt ttataataag gagattttta tgaatatcat aagtaataaa
    32761 atagatgagt taaaagaaga tttattaagt gatattatag atattgtaaa aatacctagt
    32821 gtcaaagggg agtcagaaaa tggcttccct tttggcgaaa aagtagggga ggcacttaat
    32881 aaagcattag aaatatcaga aaaattggga tttaaggtta gaaacttgga taattatatt
    32941 ggttatgcag agcatggaga tagtgatgat tatgtttgtg taattggaca tgtagatgtt
    33001 gtacatgaag gagatggttg gaagcatcag ccttataagg gagaagaaac taatggaaga
    33061 atttatggac gaggagtact agacaataaa ggacctataa tgtctgcctt atatggatta
    33121 tatgctatta aagaattaaa cttaaaatta gataagagag ttagaataat atttggtaca
    33181 aatgaagaaa gtggatttga agatatacca tattatttag aaaaagaaaa agcacctatt
    33241 atgggattta caccagattg taagtatcct gttgtatatg gtgaaaaagg catggcaaag
    33301 attagaataa aaagtaaaat caattatgaa gaagatgtat acctaggttt tatagaaaat
    33361 atgtctgaaa atgtgcttgt tacatataaa gagctgaata tagaaaatag tgatactata
    33421 ttagatataa aggtaaaata cgattttagt tataaattga aagatgtatt agatgaaata
    33481 aaagctagtt ttccaaatag tatcgatata gaggtaattt ctaattttaa tcctgtatat
    33541 tttgataaag agagtaatct tgttaaaaag cttcagctag cttatgaaag ggttacatca
    33601 ttagatggaa ctccagtaac aactaatgga ggtacgtatg caaaagtaat gccaaatata
    33661 gttccgtttg gaccttcatt tccaggacaa aaaggaatag ctcataatcc tgatgaatac
    33721 atggatatag aagatataat attaaatgca aagatatttg caaatgcaat atatgaatta
    33781 gcaaaggagt agttatgtta gaaataatag gaatgtcagt agaagacgct aaaattatag
    33841 aagattgtgg tgcagataga atagagttag ttagtgcttt aactgaaggt ggattgacac
    33901 caagttttgg tcttatagaa agtgttgtta acagtgtaaa gatacctgtg aacgtgatga
    33961 taagacatca tgcgaaaagt tttgtataca gtaaagaaga tataagtata atgcaaaaag
    34021 atataagtgt ggttaaagag attggtgcta atggagttgt atttggtgta ttggataaaa
    34081 acaatagtat agatgagaaa aatctaaatg ttttattgaa atgttgtgat aatcttgatg
    34141 ttacatttca tagagcaata gatgaatcaa atacaataga tagtgttaaa attttgaagg
    34201 attatgataa aattacgaat atattaacat caggagggaa aggaagtata gttgataaca
    34261 tacaaatgat aaagaatatg atgcttagtt ctaatcacat aaaaattctt ttaggtggag
    34321 gtcttaattt taataatata gaaaagataa aggaattgac taaggcaagt aattttcatt
    34381 ttggtacagc gattagaata aataacagtc cttttgaaga tatagataga caaaagctta
    34441 aacaactcgt aaatataatt tcaagatagt atatgaatat aaaatactta gtttaaataa
    34501 agagagtaag ttaattataa tttgggtata tgaatgttaa taggtattaa atatgaaatt
    34561 attgttgagt atgctttata acttttaagt agttttaaat ataaataatg aaagaaaaga
    34621 tagattatgg aggttttaga gggtttatat ttattaaaaa attataaagg tatattggca
    34681 tataatacac ataatttcct aaaattacca tcttatgaca tgaattcaca tattaataaa
    34741 caattaatgg agatttttgt ggatagaaat aggagtaaaa atagctttaa ataaacaaaa
    34801 acaagagtta ttaacagttt tatccacatt atccacaggt gtttgtaata atattaatcc
    34861 acataagtat caatgtaact aaaaatgata aataattcta tcaattgaat agaaaaaatc
    34921 ataaaagtaa ataataaaaa gaggattatg agagttcgtg tagaatatat tattaaagta
    34981 taaattttct tatactggca taattgaagg gagatgtatt tatgaacata attatatctg
    35041 gaaaacaaat cgaattaact gatgcaataa aaaataaaat cgaaactaaa ctaagtaagt
    35101 tagataatta cattcatcca gacactgatg taaaagttac agtaagtgcg agaaaagcaa
    35161 gacaaaaaat agaagtaaca atagctactg taaatggtcc tataattaga gcagaagata
    35221 tgcaggaaaa tttatacaca gctatagatg ttgtatatga taaattaagt aaacaattaa
    35281 aaaaatataa aaagagatta caagataaac ataaagacaa caagagtata agattccaag
    35341 atgcagagat aaatctagct gatgaacaag aggattatgg ggatgaaaat caagaattag
    35401 taattcaaag acgtaagaaa tttagtgtta agccaatgag tgaagaagaa gctgtattac
    35461 agatggaatt aataggacat gatttttata tgttcaagaa catagattca gatgaaatag
    35521 cagtagttta taagcgtaat aatggtggat atggtataat agaacatgaa taaagcaatt
    35581 tagatttaat catattagct atcgataaat aaattatcga tagcttttta aattaatatt
    35641 aaaagtatag tttttatggt aaaatgaata atagtacaat aaagaacgag gagagatgta
    35701 aaatatgagc tttatggata atttattcaa catggcagat aaaaaagaat taaaaaagtt
    35761 caataaaact gttgatataa tagattcatt agaacctaaa tttgaatcta tggcagactc
    35821 ggaacttaaa aatatgacaa atatatttaa agaaagatta gcaaatggtg aaagtataga
    35881 tgatatactt ccagaagctt ttgcagtagt tagagaagtt tctaagagag tgttaggact
    35941 tagacactac agagttcaaa tgattggagg tatagtacta catcaaggaa gaatagcaga
    36001 aatgaaaaca ggtgaaggta aaactttggt tgcaactgct cctgtttatt tgaatgcgct
    36061 tactggaaaa ggtgtacatg ttgtaacagt aaatgattac cttgcaaaac gtgatagaga
    36121 ccaaatggca aaaatctatg agtttttagg aatgagtgta ggcgttatta tacacggtca
    36181 aaatccaaaa gttagaaaag aacaatatga ttgtgatata acttatggta caaataatga
    36241 atatggattt gactatctaa aggacaatat ggtaatacac aaagagcaaa gagttcaaag
    36301 aggattaaat tatgctatag tggatgaggt tgactctata ctaatagatg aggctagaac
    36361 accacttatc atatctggac ctggagacaa atcaactcac ttatattcag atgcaaacac
    36421 atttgtttta accttaaaac cagatgatta tgaattagaa gaaaaagata aggctgtatc
    36481 tctaacagca tcgggtatac aaaaagcaga agtgtatttt aatgttgata atataacaga
    36541 tatatctcat acagaattat accatcatat aaatcaagct ttaagagcac atgtaataat
    36601 gaagaaagat gttgattatg ttgctaaaga tggagaaata gtaatagtag atgagttcac
    36661 aggtagactt atgtttggta gaagatattc agaaggttta catcaagcaa tagaggctaa
    36721 agaaggtctt aaaatacaaa gagaatctaa aacactagct actgtaacat tccaaaatta
    36781 ctttagaatg tacaaaaaac tttctgggat gactggtaca gcaaaaacag aagaagaaga
    36841 gtttaaagca atctacaaaa tggatgtttt ccaagtacct actaataagc ttatgataag
    36901 ggaagactta ccagactgtg tatacaaaag tgaaataggt aaatttaatg ctgtagcaca
    36961 agaaataatt gaaaggcaca aagtcaatca accaatactt gtaggtacag tatcaataga
    37021 aaaatcagaa ttattatcac aaatactaaa gaaaaagggc ataaagcacg aggtattaaa
    37081 tgctaagcat catgataaag aggcagaaat aatagcacaa gctggtagac ttggagctgt
    37141 tacaatagct actaatatgg ctggtcgtgg tacagatata gttctaggtg gaaatccaga
    37201 ctttttaact aagagagaaa tgagaagaaa tggatttaaa gaagaaatag taaatagagt
    37261 agatactcca atagaaggta tacctgtaaa aggtaatgaa attctatttg aagctagaga
    37321 agagtatgaa aaactatttg aaaagtttaa gcaacaaaca caagaagaac aaaaacaagt
    37381 tgtagaagca ggtggacttg ctataatagg tacagaaaga cacgaatctc gaagaataga
    37441 taaccagttg agaggtcgtg ctggacgtca aggtgaccca gggagttcaa gattctatat
    37501 aggtcttgat gatgacctta tgagattgtt tggtagtgat agaatatctg gtattgtaga
    37561 taaaataggt cttgaagaag acatgccgat agaacataga atattatcta agtctattga
    37621 aggagcacaa aagaaagtcg aaggtaaaaa ctttggaata agaaagcatg tacttcaata
    37681 tgatgatgtt atgaataaac aaagagaaat aatatatgca gagagaaaac gtgtattaga
    37741 aggtgaagat ttacaagaac aaatacaatc aatgactcat tctataatag aagaagcagt
    37801 tactctttat acacaagata aaggatttga tgaagaagga ttcaaagaac atatgtataa
    37861 tctgttcttg ccaaaaggta gcatagaaat accagaaata gaaaaactta atccagttga
    37921 aataacagaa aaagtttatg aaatagctat gaagatatat acttcaaaag aagaacaagt
    37981 tggctatgag agaatgagag aagttgagag agtaatctta cttcaagctg ttgataacca
    38041 ttggatagac catatagatg ctatggacca attacgccaa ggtattggtc ttcgtgctgt
    38101 aggtcagcaa gacccagtta ttgcttataa aatggaaggt tttgatatgt ttgatgaaat
    38161 gaataaacat atcaaagaag atactgtaag atatttattt aatataacaa tagaaactcc
    38221 agtggaaaga aaagcagttg tagatgttga aaatctatct tctccatcag atggaacact
    38281 tccaacaagt aaaactgtta aaaaagatga aaaggttgga agaaatgact tatgcccttg
    38341 tggaagtggt aaaaaatata aaaactgttg tggaagataa tctcggagga ttaaatgtta
    38401 aagttacaag agtatagaca tagtatggag cagatggctt caaatttaga agaattgagg
    38461 gtttctcttt gacatagctt cattgatgaa aaatatacaa attaatgaag aaaagatgaa
    38521 tcaacaagat ttttggaatg ataatgaagt cgctcaaaga gtacttcaag aaaataaatc
    38581 tttaaaagag acattagaag aatatgaaag cttaaaaagc ttattagaag atatagaagt
    38641 attaattgaa ataggtttag aagaagatga tgattctgta gaaagagata tagaaaagtc
    38701 catagaatca atggaagaaa aattatctga aatgaaaata aagactcttc taaatggaga
    38761 gtatgataaa aataatgcta tattatctat aaatgctggt actggtggct tagatgctca
    38821 agattgggct caaatgcttt tgagaatgta tataagatgg tcagaaagta agggttataa
    38881 agttaagctt ttagatataa tatcagaccc tgaagcaggg ataaagacag ctacaattct
    38941 tgtagaagga acaaatgctt atgggtattt aaagagtgaa aaaggagttc atagattagt
    39001 gagaatatct ccatttgacc catcaggaaa aagacatact tcatttgcat ctatagatgt
    39061 aacaccagag ttagatgaaa atatagaagt agaaattaat ccatcagatt taaagataga
    39121 cacatataga gcttctggag ctggagggca acatgtaaat acgactgatt cagcagttag
    39181 aataacacat atccctactg gtgtagtagt tcagtgccaa aatgaaaggt ctcagcatct
    39241 aaataaggac agagctatga gacttttaat ggcgaaattg atagaattaa aagagttaga
    39301 gcaaaaggaa aaaatagaag atatacaagg caaatactca caaataactt ggggtagtca
    39361 aattaggtca tatgtttttc aaccatataa attggttaaa gaccatagaa caaatgctga
    39421 gtttggcaat gtagatagtg taatgaatgg aaatatagac ttgtttatta atgaatactt
    39481 aaaaatgaat aaaatagttt aataaatatg cactgtaatt aagatatatg tataagaagg
    39541 ttagtgctta atatgcactt acagcactta ggctgaggac gataaccctc agccttcgtt
    39601 ctgttttgtt atacttatga aaaactaaca ttaggagtga ataaattgga tataaatcaa
    39661 atcttaaaaa aagagtttaa tcttagagat gaacaaataa ataatacatt aaaacttatt
    39721 gatgaaggaa atacaatacc atttatagct agatatagaa aagaaatgac tggcgaaatg
    39781 agtgatgtta cactaagaga attttatgaa aaattaatgt atttgagaaa tcttcaaagt
    39841 agaaaagatg atgtagttag actaatagat gaacaaggga aattaacaga tgaaataaca
    39901 caaaatatag aaaaagcaaa aacattacaa gaagtagaag atatatatgc tccatacaaa
    39961 caaaagaaaa gaacgagagc aacaatagca aaagaaaaag gattagaaaa tttagcattg
    40021 tcaatactag agaataattt agataatata gaaatagagg caaagaatta tcttgatgaa
    40081 gagaaagaag ttctttctat tgaagatgct ctaaaaggtg ctagagatat aattgcagaa
    40141 ctagtctcag atgatgctaa gattagaaag tatataagag aattagcttt aagagaaggt
    40201 atgatagtta gcaaaagtgc tactgatgag aaatcggttt atgatatgta ttatgattat
    40261 agcgaagcag taaaaagtat ggctccacat agagttttgg caataaacag aggagaaaaa
    40321 gaaagcttcc taaaggttaa gttggaaata aataatgata aagttttaaa ttacataata
    40381 aacgaatatg taaatgataa aaatttcaaa aataaagaag aaatcgtaag ttcaatagaa
    40441 gattcttata aaagattaat tttcccatca atagaaagag aaataagaaa tcatttaaca
    40501 gaaatagctc aagaaagagc tataagtgta tttggaaaga atgttaaaag tttattgctt
    40561 caaccaccag ttaaagacaa agtagttatg ggatttgacc ctgcatttag aactggatgt
    40621 aaaatagctg tagtagataa aaatggtaaa ctactagatt atacaacagt atatccaaca
    40681 gaccctcaaa atgatgtaga gggagcaaag aaagttttaa aaggattgat tgaaaaatac
    40741 gatatagata taatttcaat tggaaatggt actgcatcta gagaatcaga aacttttgta
    40801 tcagaaatga taaaagaaat agatagcgaa gtgcagtatg tgatagttag tgaagcagga
    40861 gcatctgttt attcagcatc tgaacttgca aatgaggaac atccagatat aaatgtatca
    40921 ataagaggag ctatatctat agctagaaga ttacaagacc cattagcaga gcttgtaaaa
    40981 atagacccta aaagtattgg tgttggtcaa tatcagcatg atttaaacaa aaagagatta
    41041 gaagaagtac tagatggagt tgttgaagat tctgtaaata gtgttggagt agatttaaat
    41101 actgcatcat actccctact agaacatgtt gcaggaataa gtaaagctat agcaaaaaat
    41161 ataatagcat atagagaaga aaatggagat tttacgtcaa gagcacaatt gaaaaaagta
    41221 aaaagactag gacctcaagc atttactcaa tgtgctggat ttatgagaat cttagagggt
    41281 aaaaatccac tagataatac aggtgtgcat cctgaaagtt atgatatatg taaaaaaatg
    41341 attgagataa ttggatattc tttagatgat gtaaagaata agaatatagg tgaaatagat
    41401 gaaaaaataa aagaaattgg cttgagggaa ttaagtgaaa aactggaagt aggacaagtt
    41461 acattaaaag atatcatagc tgaaatcaaa aaaccaggaa gagacccaag agaagaaggt
    41521 ataaaaccta tacttagaac tgatgtctta aaaattgaag atatacaaga gggtatgact
    41581 ttaaaaggta caataagaaa tgttgtagac tttggtgcat ttgtagatat tggaataaaa
    41641 aatgacggat tagttcataa atcagaaatg agtaatagtt ttgtgaaaga ccctatgagt
    41701 attgttacag taggtgatat agtagatgta aaagtcattg gaattgactt aaacaaaaaa
    41761 agagttgcct tatcaatgaa aaaatagata agtttatgta tggttataat acttagattc
    41821 tatatttgta aataagatta ctcgagtgta taaatgttaa aaatttgtaa aagtatatag
    41881 taagaaaata tattaaaata agaacgatag tcttgaaaat caggaggaaa atgaatgatt
    41941 tttataaaaa atggtaaaat aaataccata acaaatggga taattcatgg agatatactt
    42001 atagatgaag gaaaaataat agaaattgga gaagatttga tagctccttt agatgtagag
    42061 gtaatagatg ctagtaataa acttgtgttt cctggattta tagatgctca tactcactta
    42121 ggtttatggg aagatggtat agggtttgaa ggagcagatg gaaatgaaga gactgaccca
    42181 ataacaccac aattaaatcc tatagatgga attaatccaa tggatagaac ttttaaagaa
    42241 gcttttgaag gtggaataac ttctgtttgt acaacccctg gaagtgcaaa tgttatgggt
    42301 ggtcaatgta tagctataaa gacatgtgga aaaagaattg ataaaatggt aataaaaaat
    42361 ccagttgcat ctaaaattgc ttttggagaa aatccaaaga gctgttatgg tcaggatgat
    42421 aaatctcctc aaactagaat ggctatagct gctttactta gagaaaacct aaaaaaagca
    42481 gaagaatatt tagaggatat tgatatgtat gagagtcatg atgatgaaga ctgtgaaaaa
    42541 cctgaatatg atataaaaat ggaaagttta ataccagttt taagaagaga aattcctttt
    42601 aaggcacatg ctcatagagc agatgatatg tttacagcaa taagaattgc aaaagaattt
    42661 aatttaaagc ttacattaga ccattgtaca gaaggtcatc ttattgtaga tgaattagtg
    42721 gaagaagggt ttcctgtaat agtagggcca tctttgtcag aaaggtctaa atttgagctt
    42781 agaaatttga catttaatac agcaggaata ctttcaaatg caggtcttga tgtatgtata
    42841 atgacagacc atccagtaat accagttcaa tatcttccaa tatgtgcagg tattgcagta
    42901 aaacatggta tgaaagaaga aaaagcaata gaatcaatta cgataaatcc agctaagact
    42961 ttaggaatag aagatagagt aggctctata gaagttggta aagatgcaga cctagtaatt
    43021 tgggataatt ctccactaga gattcaaagt aatgtacttt acactataat aaatggcaaa
    43081 gtagtgtatg agaaaaaata acttgacaat tattaaaaaa ttaactaata taagagtgta
    43141 aggaaaatta aaaaatcaaa gaagatcttc ggggcagggt gaaattccct accggcggta
    43201 tagcccgcga actacttaag tggttgattt ggtgagattc caaagccgac agtaaagtct
    43261 ggatgagaga agatatttat ggaagataaa taagtttatt ttagtttgtt taaactatat
    43321 ccataaaaac tcgaagatag ggtcttcgag ttttttgttt ttcctaaaaa atataaacat
    43381 attgattagg gggaaaataa ttatgcaaaa cacagcaaaa agaggcaata tgatttcaac
    43441 aagaacttta gtagtaatgt caattttatc tgcaatatct tatttattaa tgtttattca
    43501 agcaccaata ccaggtatat ttccagattt cttaaaaatt gacttatcag atattccagc
    43561 aatattcgga ggaatgtctc taggaccatt agttggattt ggaatagttg tagtaaaaaa
    43621 tgtgttacaa gcaataacag caacaacaac tggaggaata ggagaatttg cgaatatatt
    43681 aattggtggt tcttatgtaa tgatactttg ttattcatat aagagaagtg gagatttaaa
    43741 aggtgtactg attggttttg tattaggaac aatatctatg actataattg gttcattagt
    43801 aaattatttt gtaatgttgc cactttatgg tcaacttatg ggactagatg ctataatagg
    43861 tttaggttca gctataaatc ctcaagttca tgatttattc acttttgtaa tttggatgat
    43921 agctccattt aatgtgctga aggctgttat aatatcttta gtaacattgc cactttataa
    43981 aaaaatggga aatataataa agaaataatt agacaagaaa atataaaatt aaatgtattg
    44041 aatagccata atttatttat aacttcagtc ctatattgtg attgatatat taaatatatt
    44101 atggctttat taa
//
LOCUS       DAENCV010000021        43964 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAENCV010000021 DAENCV010000000
VERSION     DAENCV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.021"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(72..845)
                     /locus_tag="KQD82_001081"
     CDS             complement(72..845)
                     /locus_tag="KQD82_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG4379563.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1231)
                     /locus_tag="KQD82_001082"
     CDS             complement(845..1231)
                     /locus_tag="KQD82_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4379564.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFYIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="KQD82_001083"
     CDS             complement(1256..1573)
                     /locus_tag="KQD82_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG4379565.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="KQD82_001084"
     CDS             complement(1585..1845)
                     /locus_tag="KQD82_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG4379566.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="KQD82_001085"
     CDS             complement(1845..2141)
                     /locus_tag="KQD82_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG4379567.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="KQD82_001086"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="KQD82_001086"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG4379568.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="KQD82_001087"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="KQD82_001087"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG4379569.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGNSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="KQD82_001088"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="KQD82_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG4379570.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="KQD82_001089"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="KQD82_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG4379571.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8034..9140)
                     /locus_tag="KQD82_001090"
     CDS             complement(8034..9140)
                     /locus_tag="KQD82_001090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG4379572.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="KQD82_001091"
     CDS             complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="KQD82_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG4379573.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="KQD82_001092"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="KQD82_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG4379574.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="KQD82_001093"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="KQD82_001093"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG4379575.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="KQD82_001094"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="KQD82_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG4379576.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="KQD82_001095"
     CDS             12234..12941
                     /locus_tag="KQD82_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG4379577.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /locus_tag="KQD82_001096"
     CDS             12910..13539
                     /locus_tag="KQD82_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG4379578.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="KQD82_001097"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="KQD82_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG4379579.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15125..17020
                     /gene="mnmG"
                     /locus_tag="KQD82_001098"
     CDS             15125..17020
                     /gene="mnmG"
                     /locus_tag="KQD82_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG4379580.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="KQD82_001099"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="KQD82_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG4379581.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17895..18680
                     /gene="noc"
                     /locus_tag="KQD82_001100"
     CDS             17895..18680
                     /gene="noc"
                     /locus_tag="KQD82_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG4379582.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18815..19588
                     /locus_tag="KQD82_001101"
     CDS             18815..19588
                     /locus_tag="KQD82_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG4379583.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENIDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19590..20453
                     /locus_tag="KQD82_001102"
     CDS             19590..20453
                     /locus_tag="KQD82_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG4379584.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20486..21628
                     /locus_tag="KQD82_001103"
     CDS             20486..21628
                     /locus_tag="KQD82_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG4379585.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21762..22337)
                     /locus_tag="KQD82_001104"
     CDS             complement(21762..22337)
                     /locus_tag="KQD82_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG4379586.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22597..23889
                     /locus_tag="KQD82_001105"
     CDS             22597..23889
                     /locus_tag="KQD82_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG4379587.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23994..24596
                     /gene="yedF"
                     /locus_tag="KQD82_001106"
     CDS             23994..24596
                     /gene="yedF"
                     /locus_tag="KQD82_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG4379588.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24669..24932
                     /locus_tag="KQD82_001107"
     CDS             24669..24932
                     /locus_tag="KQD82_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG4379589.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24947..25132
                     /locus_tag="KQD82_001108"
     CDS             24947..25132
                     /locus_tag="KQD82_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG4379590.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTRCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25235..26431
                     /locus_tag="KQD82_001109"
     CDS             25235..26431
                     /locus_tag="KQD82_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4379591.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26645..26923
                     /locus_tag="KQD82_001110"
     CDS             26645..26923
                     /locus_tag="KQD82_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG4379592.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26960..27394
                     /gene="ssb"
                     /locus_tag="KQD82_001111"
     CDS             26960..27394
                     /gene="ssb"
                     /locus_tag="KQD82_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG4379593.1"
                     /translation="MNKVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27413..27640
                     /locus_tag="KQD82_001112"
     CDS             27413..27640
                     /locus_tag="KQD82_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG4379594.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27783..28103
                     /locus_tag="KQD82_001113"
     CDS             27783..28103
                     /locus_tag="KQD82_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG4379595.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28111..29067
                     /locus_tag="KQD82_001114"
     CDS             28111..29067
                     /locus_tag="KQD82_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG4379596.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29078..31072
                     /locus_tag="KQD82_001115"
     CDS             29078..31072
                     /locus_tag="KQD82_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG4379597.1"
                     /translation="MSNKQAFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31073..31522
                     /locus_tag="KQD82_001116"
     CDS             31073..31522
                     /locus_tag="KQD82_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG4379598.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31541..32869
                     /gene="dnaB"
                     /locus_tag="KQD82_001117"
     CDS             31541..32869
                     /gene="dnaB"
                     /locus_tag="KQD82_001117"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG4379599.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33074..33382
                     /locus_tag="KQD82_001118"
     CDS             33074..33382
                     /locus_tag="KQD82_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379600.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33464..34753
                     /locus_tag="KQD82_001119"
     CDS             33464..34753
                     /locus_tag="KQD82_001119"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG4379601.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35079..35154
                     /locus_tag="KQD82_001120"
     tRNA            35079..35154
                     /locus_tag="KQD82_001120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35112..35114,aa:Lys,seq:ttt)
     gene            35161..35235
                     /locus_tag="KQD82_001121"
     tRNA            35161..35235
                     /locus_tag="KQD82_001121"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35194..35196,aa:Glu,seq:ttc)
     gene            35240..35315
                     /locus_tag="KQD82_001122"
     tRNA            35240..35315
                     /locus_tag="KQD82_001122"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35273..35275,aa:Val,seq:tac)
     gene            35349..35423
                     /locus_tag="KQD82_001123"
     tRNA            35349..35423
                     /locus_tag="KQD82_001123"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35381..35383,aa:Thr,seq:tgt)
     gene            complement(35806..36798)
                     /locus_tag="KQD82_001124"
     CDS             complement(35806..36798)
                     /locus_tag="KQD82_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4379602.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36807..37865)
                     /locus_tag="KQD82_001125"
     CDS             complement(36807..37865)
                     /locus_tag="KQD82_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG4379603.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37990..39453
                     /locus_tag="KQD82_001126"
     CDS             37990..39453
                     /locus_tag="KQD82_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG4379604.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39555..40352
                     /locus_tag="KQD82_001127"
     CDS             39555..40352
                     /locus_tag="KQD82_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4379605.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40365..42467
                     /locus_tag="KQD82_001128"
     CDS             40365..42467
                     /locus_tag="KQD82_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG4379606.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42535..43014
                     /gene="rlmH"
                     /locus_tag="KQD82_001129"
     CDS             42535..43014
                     /gene="rlmH"
                     /locus_tag="KQD82_001129"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG4379607.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43163..43474
                     /locus_tag="KQD82_001130"
     CDS             43163..43474
                     /locus_tag="KQD82_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4379608.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43522..43788
                     /locus_tag="KQD82_001131"
     CDS             43522..43788
                     /locus_tag="KQD82_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4379609.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNF"
BASE COUNT        16584 a         5543 c         6997 g        14840 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 actttatctc taaaatactt ttttatttct cctactatag ttggtgttgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatataa aacacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttctt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat catccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttaacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctatagcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcatccttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatctcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 gcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aacgagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagc tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctcttagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc aatatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaagtt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctagtaaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccgtctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 ttcccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttca tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atatattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggcaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcctca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccttt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatatat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat ttcaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtat tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagta tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aattttcgta
     8461 tcattaatta taaaaatagc atttttttcg ttaaatccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caaccgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta ggatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatta acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctgagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt gctattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag aattttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag aaggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg cggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgagccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta cagatagctt agttgagctt ggccttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag taagatttag tgataaaaca tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaaaggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggtttctaaa taaaaaggct gctgtagaga aagaatttga
    16561 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaagg cgctggagat gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa actagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgactgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaaaatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
    17761 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
    17821 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
    17881 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
    17941 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
    18001 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
    18061 aactaattgc aggagaaaga agattaagag cagctaaatt agcagaactt aaaaccatac
    18121 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
    18181 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
    18241 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
    18301 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
    18361 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
    18421 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
    18481 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
    18541 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
    18601 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
    18661 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
    18721 cttgtaatat aaactgattt atagacatta tttatactta aaaaacacct attcaacatt
    18781 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
    18841 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
    18901 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
    18961 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
    19021 tattaaagaa gcaataataa gtactgaatt tgagaatata gatgtagttc catcagctac
    19081 tgaactatca ggtgcagaga tagagcttac atctaaaact aatagagagt atattttaaa
    19141 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
    19201 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
    19261 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactataa aattagtaaa
    19321 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
    19381 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
    19441 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
    19501 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
    19561 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
    19621 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
    19681 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
    19741 atgaagaaaa gataaaagta ttgtctgact ctataaaaaa ttatggtgtt ttacagccaa
    19801 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
    19861 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
    19921 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
    19981 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
    20041 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
    20101 gacaagatgt tatagaaatg atagacagtg gtagaattac agcaggccat ggtaaggctt
    20161 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
    20221 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
    20281 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
    20341 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
    20401 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
    20461 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
    20521 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
    20581 aggtagagcc ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
    20641 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
    20701 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
    20761 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
    20821 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
    20881 atcaataaag aataatacta aattgattgt aacaacacat gcgtctaatg tatgtggaac
    20941 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
    21001 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
    21061 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
    21121 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    21181 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    21241 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    21301 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    21361 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    21421 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    21481 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    21541 agtaaggttc agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    21601 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaaag
    21661 gggatatttc aaaataaaaa tttgaatcat ctcctttttt gtacaaaaaa gaaaaggggt
    21721 tcctaagaac ctctaatcta tttgataata gtttcaagaa attactttaa gtaatctcct
    21781 tcttcaagaa ctatatgacc actttcgtaa ttagaaccat caacagtaat ttttatttgt
    21841 tcaacattca tattttttat gtaagtacga gcaactgaat caagcattaa agtttctgca
    21901 tcactaccta agtttgactt tgtaaagtca gagctaacat ctaatatacc agtttttaca
    21961 ccatcaatgt cttttgtagt aaatgaattt aattttgcag attctggaac aactttaagt
    22021 ttttgaagtt cttcaaataa agtgttctca tcaattttat ttaaatcaac tttattttca
    22081 attaaatctg tcttatctac atcaaaagag taaatagtag ctgattgagc tttagtttct
    22141 tcttttggag taggtttagc tttgttatta ggaacctctt tttcagtatt gttttcttct
    22201 tttggttgct caacatttgt attgctctta gtttcttttt tagatgaatc acttccattt
    22261 tgagcattag tacaaccaac tgcgaatata gatatagata atatacttaa cactaacatt
    22321 attttcttat ttttcataaa aatttctcct ctcttaaaaa ctattcttat atataattat
    22381 agtcgttatg agcaaatata tgaatacaaa atagtaacaa atttaaaaaa tgatttattt
    22441 aaattaataa aactcaaaat aaactaaatt ttagtcattt ttatgggaaa tcatttaatg
    22501 tacagtgaaa atattgtaaa ataaaagtag tgtttttaaa ataaatataa gttataatta
    22561 tattataaaa aaatgtcttc aaaaggtggg aatagtatgg aaattttgag cttaggagaa
    22621 aagattaaaa agttaagaaa agaaaaaaac atgacattga aagaattagc tggagatagg
    22681 ataacagcag cacaaataag ccatatagag agagataaat ctcatactag ttatgaactg
    22741 ttggaatatc taggcgataa gctagatgta agtgtagaat acttattaga gaccaaggaa
    22801 atgcaatcaa aaaagataac tgataattta atactacaga gcgaaattta tataaaagaa
    22861 aatgaattaa acaaggctga ggaccaaata aaagaagttt tagatatatg taagagatac
    22921 gatttaatgg agaactatgg cagatgtaat tttttaatgg ctaatattaa tttgaaaaaa
    22981 tctaattata atgatgctat agaaaacttt gaaaaggcat tgtacttctt tataaaaaat
    23041 agtgatagtg aaaatatact taagtgttat ttaaacatag ggaaaatata tatgcaggaa
    23101 gacttttata aaggtgctat aagccacttt aattttgcag aggaaatttt gtcagagagt
    23161 aaatttgagg atgttaacat acacaaggaa ctatatagta agatggcata ttgttacata
    23221 aaactagata atccaaatat gtcattgaag tacatagaaa agataaatga aatagataat
    23281 agaaatgata gaaaagaaga tgtcgatatt ttggtgttaa aagcaaataa tatgcttgag
    23341 attggaaaat atgaagaatc aagagattac tttaaaaaag cactaaaaat acttgagagt
    23401 gaggataata aaactggatt agcaaatgtg tatttaacaa tttgtgatgt ttacagaaaa
    23461 ataggtgaaa cagatagagt acttgaatat tcacaaaagg tatatgacat aaagaaaaat
    23521 gatgaagatg aatacatgat gtcaggatta tttaagataa tagaagcata tatagataaa
    23581 gaagattatg atttagctag aaaatattgt aagatagcct tagcttcatc aataaaaaat
    23641 aaaaataaat tcaatgaata taaagcattg agattctatg ctaatatgta taaaaatcag
    23701 aatgaagtaa ctcttgcaat tgaatatctg attaaatgca taaaaatagt atcagattta
    23761 ggcaacaata gaatattggc caatttatat atagacttag gtcaattata ttcaaatata
    23821 tcaaaagaaa aagagttaga atattatcaa aaaggtgtat ttatgtacaa aaacttagaa
    23881 ataatgtagg gtaaataaac ctataagggt atagaaacaa tctataacaa gcaagataat
    23941 atctatgata aaatataaat tgtatgaata tttttaagga gatgaatcag acaatgagta
    24001 taaaaataga tgcgagaggg cttgcttgtc ctaaaccagt tataaataca aaaagagaat
    24061 tggataacct agaagaaggt gttgtagtta caatagttga taatgaaaca gctaaggaga
    24121 atatattaaa acttgctaaa tctttaagtt gtgaagcaaa tattgttgac gaaaaagaga
    24181 attttatatc tatagaaatt agaaaaggta aaaatatact agataaacaa aatgttgtag
    24241 ataataaaga aagtgaatta gacaatacat gtatatttat atcttcagat aagatgggac
    24301 ttggaaatga cgaattagga aaagtgttaa taaaaggatt tatatataca ttaacagaat
    24361 ctaaacctta tcctaagtat gtacttttag ttaatggagg agtaaaatta agtgctgaaa
    24421 atgaagaaac catagaaaat ctaaaaatac ttgagaatat gggtgtagaa gttttatctt
    24481 gtggtacttg tttagattat tataatttaa aagacaaatt acaagtaggt acagttacaa
    24541 atatgtatac tatagttgag acattaaaga atgcatcaaa taccatttct atataatgta
    24601 tgtatgttta ataatataaa actaacaata aagattaact caaatagata gtcagaaagg
    24661 attaagtaat gaatcaaatg tatatagtgt catttaattc gacacatcat gcgataagat
    24721 cagaaaagtt atttggtgaa aatagtctaa aagtaatggc tcttccaaca ccaagagaaa
    24781 taacagcaag ttgtggaata tcaattaaat tttcttttga agatatggaa aagataaaaa
    24841 ctattttggt tgaaaataat gtagatataa aaggaattta ttgtataagt aaattagaaa
    24901 atggttcaaa agaagtagag aagttagatt aggaggctaa ttatttatgc caatggattt
    24961 aaaaataggt gatattgttg aattaaaaaa gcaacatgca tgtgggtgta aagagtttga
    25021 aatcataaga acgggaatgg atataaagat aaaatgtact agatgttcaa ggctaataat
    25081 gcttgataga gagaccttag aaaagagagt aaaaaagcta ataaaaaagt gataaaaatt
    25141 taacacttaa aaaatgtcaa ataccgtata ttaatatcca aaattttggt ataataagaa
    25201 ttgaagaaaa cattttcatg ggggtgcttt aattatggca gttaaatatg caaaaagaat
    25261 gcaaggatta caaggatcag aaatacgtga gcttttaaaa cttactcaac aaccacaaat
    25321 aatatctttt gctggtggaa tgccggctcc agagttattt ccagttgagg aaatgaaaaa
    25381 agtatcagtt gcagtgcttg aagaaaatgg aagatcagct atgcaatata ctacaacaga
    25441 aggatatgaa ccattaagag aaaaaatagc agctagaatg aatgataaaa acaaaactaa
    25501 tgtaaacaaa gatgatatat tagttacaag tggttctcaa caaggtctag attttgcagg
    25561 aaaagtattt attgatgagg gtgatgtaat tttatgtgaa agtccatctt acataggagc
    25621 tataaacgca tttaaatctt atcaacctaa attcatagat gttccaacag attctgatgg
    25681 aatgataatg gaagaattag aaaagatact agaaacaact gatagaataa aaatgattta
    25741 cgtaatacct gacttccaaa atccaacagg aagaacttgg ccacttgaaa gacgtaaaaa
    25801 attcatggaa atagtaaata aatttgaaat accagtaata gaagataatc catatggaga
    25861 tttaagattt gaaggagaga ctttaccatc tttaaaatca atggatacaa aaggattagt
    25921 aatattctta ggaactttct ctaaaatatt ctgcccagga tacagattag gatggacttg
    25981 tgcttctcca gaaattttat ctaagtttaa ctttgcaaaa caaggtgcag acttacaagc
    26041 atcaactata tctcaaatgg aagtaagcaa atttatggat atgtatgacc tagatgcaca
    26101 tgttgacaaa ataaaagcag tttatgttaa acgtagagat gtaatgctta agactatgga
    26161 agaagagttc ccagaaggat tagtatttac tcatccagaa ggtggattat ttacatgggt
    26221 agagcttcca agtaatttaa atgctaaaga attaatgcca aaatgtttag ataagaatgt
    26281 tgcttatgtt gctggaggag gattcttccc taatggtggt agagaaaata ctttcagact
    26341 taactattca aatatgccag aagaaaaaat aatagaaggt ataaaaaata tagcagctgt
    26401 attaaaagaa gctatgggtg tagaagctta attaaaaata ataacttata tataatacaa
    26461 gggatggttg ttgccatccc ttgtttgttt gaaaagtata taattcacct tatactaaac
    26521 tttgtccaaa atttaaattg acagaaataa gaataaatga taaaatgtgt aggtatgaga
    26581 ttttagtctc atttctctgc tctaagagtg tagagccgtt aagtccaaag ggaggtgaat
    26641 tatagtgaga aattatgaat tagtttatgt ggtaaagcca aactctgatg aagaagtaag
    26701 agaggctata ctaaacaaag ttaaggaagt tgttgcaact gacggagaaa tagttaaagt
    26761 tgatacttgg ggaactaaaa aattagctta tccaatagct aagtttacag aaggtttcta
    26821 tgtattagtt aacttcaaat cagcagtaga cgttcctaaa gagatagaca gaaacttaaa
    26881 gataaatgaa aacgtaataa gacacatgat agttgttgct taataactgg ttttattata
    26941 aaaaataggt ggtgttttta tgaataaagt tgtattagtg ggaaggttga ctagagaccc
    27001 tgaattaaga tacatcccag gtacaggaac accagtggca tcctttacaa tagccattga
    27061 tagagactat gtaaaaaaag atggatctaa agaaactgat tttatacctg ttgaagtaat
    27121 gggtaaatct gctgaatttt gtgctaacta cataactaaa ggtagattag tagcacttca
    27181 aggatctata agagtagaca attatcagac acaatcaggt gaaaagagaa cttttacaaa
    27241 agtaagtact agatcagtac aagctttgga tagtaagaat aaatcggaaa attcatatag
    27301 agagagtgct ccagcttttg agccaagctt tgaacctcaa ggtttagacc cacaaggttt
    27361 ccaagctata gatgatgacg atataccatt ttaaaaagga gggaaaaaag tcatgatgaa
    27421 taaaaaaaga cgtaagaaaa aaagagtttg tcaattttgt gctgacaaaa acgctaaaat
    27481 agattacaaa agtactcaaa gattacaaaa gtatataact gaaagaggaa agatattacc
    27541 aagaagaata tctggaactt gtgctaagca tcaaagagaa ttaacagttg ctataaaaag
    27601 agcgagaaat atagcacttt taccatatac attagactaa aaaaatgagc cttgtgctca
    27661 ttttttatta tatgtattta tatatctaaa tgaggcgagc tataaagctc gtttttttgt
    27721 ttatatactt aaatatatta ttatttatga tataatatta tatattaagg agggttgtaa
    27781 acgtgaaaat agataaaggt tctgagataa cgagaaacat aaagataata gaatggatga
    27841 aaacagaaat attaatgagt gtaagtgatt tgtttaattt attgtttaaa ggggtaaaac
    27901 cattagatga agcaattcaa gatactttag caaatataat aatgataaca tatctcttag
    27961 caaagagatt aggaattagt tttagagatg tggattataa agtcaaggaa aaaataaaaa
    28021 ttggtataga tgaggaccat agcgttgaaa gatggtatgg ggatttatct aatttaaaaa
    28081 aacatatgga taatagggag tgatattaaa ttgaataata aaattagact atctaaggca
    28141 atgtctataa tagttttagc tataataatc gctttagtaa tagcctatat tccaatgcta
    28201 ggaatattta gtatgttagc agctgttcca tatgtaatta taggtgctat tactgataga
    28261 aagtattctc ttatatctat tttggttaca ttttgcgtac tgattctatt tgcagatatt
    28321 tcttacgcat taaatatatg tataatgtat tctttaccag gtatagtaat aggaaagatg
    28381 ctaaagagaa gctttgaaca agaggacagt aataaattcg aaccgatata tggtggaacc
    28441 ataatatttg tactttcaat gcttgtatat ttttttgtat taaagacatt tttaaatgtg
    28501 aatctattag atgaagtttc taagatgata tcagaaattg taaatattca aaaaaatagt
    28561 ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc cagatgagat tgtaagttat
    28621 tttacaaata tgctaccaat gatgttattt ttacaagggt tgttatcagc gtttgtcaca
    28681 tattatttga gtgtattttt tataaaaagg attacaaaga tgaatataag atttcctaag
    28741 tttgctgatt tttatttacc gggaaatgct atactcacaa ctttcttact ttatttatta
    28801 gtcttattta ttcaaattat tggttcaaaa ttatatacag aattaataat gactaattta
    28861 cagctagttt ttaatttaat gtttgtaatt caaggtatag cagtttgcat ttatttctta
    28921 aagaaatgga taagacaggg accaaataaa attatgtttt tttcagggat aattttatgt
    28981 ttatttggat ttatgggaat atcttttgtt ggtatggtag atagtataat tgactttaga
    29041 aaggtaagaa gctataaatc cacttaggag gatagaaatg agtaataaac aagcctttaa
    29101 attaaatatg ccagaaataa atttatatat aattgttatt ggtatttcta gtataatttt
    29161 gctttattac aacttgtatg tagggtgttt atttttctgt atttttgtat atatggtttt
    29221 tcacaattgg agaactacta atattagacg tcatgaatgg acagagtata ttcaaaattt
    29281 atctttagat atagatgaaa caactaaaaa agccataata aatcttccaa taccattatg
    29341 tattttagaa tttgatggaa atatatcctg gtataatgga aagttttatg atatgattgg
    29401 tcaaaaagat ttacttgata aaaatataga ggatatagtt aaaaatctta atttaagaaa
    29461 agtattaaat gaaaataaag agatgtacac agaaataaac tataaagaaa aagaatatac
    29521 aataatttat aatgttataa aaaatgatca agaaaaaaat cctaaatatt tgatgatatt
    29581 atactggata gacaagactg aatatttaaa agtaaaacaa aattatgatg atgaaaaaaa
    29641 tgcaatgatg ctaatacaag tagatggata tgatgaagta ttaaaaagtg cagcagaaga
    29701 taagagagct ttaattaatg ttgaagtaga gaaaatactt tctgctttag aattaaattc
    29761 aaatggtgca ttgagaagaa cttcaaaaga caaattcttc ttggtaatgc ataaaaaaga
    29821 gttaaaaaaa ttggaagctg agaagttttc tattttagat acaataagac atattgatta
    29881 tggaaataat cttcctgtta ctataagtat aggaattggt atagatggag atacattaaa
    29941 tgagaaccta aaacttgcta cgggtgctct cgatttagca cttggtagag gtggtgacca
    30001 ggctgttgta aaaacaaaag ataaatttgt gttttatgga ggaaaatcta aagctgtaga
    30061 aaagaaaacc aaagtaaaat ccagattaat aggtcatgct ttaagagagg ttatacaaca
    30121 aagtgaccag gtttatataa tgggacataa atatcctgat atggatgcta tgggagcagc
    30181 agttggtgtt tatgatatat gtaaatcctg taacaaaact gcaaacatag ttttacaatc
    30241 tgtaaatgaa tctatagaaa tatttataaa taaaataaat gaaaataatt actataaaaa
    30301 gttgtttatt ggaaaagagg aagctattga caactgcact aaaaatacat tagttgtagt
    30361 agttgatact catagaccaa attatactga atgtgaagaa ctgttaaagc tatcagagaa
    30421 ggtagtagtt atagaccatc atagaagagg tgtagagttt ataaatgatg cagtgctttt
    30481 attccatgag atatatgtat cctcaacgtg tgaaatggtt acagagttag ttcagtatat
    30541 ggatgaagat gtaacaataa ataaacttac agctgaaggg cttttagcag gtataagtct
    30601 agatactaaa aactttgcat tcaaaacagg tgttagaact tttgaagctg cttcttacct
    30661 tagaaaagtt ggagcagata ctatagaggt aaagaaattc ttcaattctg atgtaaaaga
    30721 tttcataata aaagctgaaa taattcaaag tactaaaata attaataata gaatatgttt
    30781 agcatattca agtactgaga tagatagtat aaatgttata atagctcaaa ctgctgatga
    30841 gttattaaat atcaaagaag tagaagcttc ctttgtatta ggtgaaaaag atgatactat
    30901 atttataagt gcgaggtctt taggacaaat aaatgtacat gtacttatgg aaaaattagg
    30961 aggcggggga cacatagata tagctggagc acagttaaaa aatgtttctc taaaagaagc
    31021 ttataagatg gtaaataaaa taatagagga gtatttggag gaggaagaat agatgaaagt
    31081 tatattatta aaagacgtaa agggaactgg taaaaaggga gaaatgaaag aagtaagtga
    31141 tggatatgca agaaatttct tatttcctaa aaagatggct gtgcaagcag atagtgtagc
    31201 aataaaagaa ttaaatgaaa agaataaatc taaagaaata aaagcacaaa aagagtatga
    31261 agaagcagtt ttattaggta agcaaatgga agaaattaat atagaaatat attcaaaagc
    31321 aggagaaggt ggaagattat ttggttcaat aacttctaaa gaaattgctg agcaattaaa
    31381 gaaacaaaaa gacatagatg tagataaaag aaaaatatta ttagatgaac caataagatc
    31441 tttaggttca actttcgtag aaataaaaat acatcaaaaa gtaactacta aaataagagt
    31501 ggatgtaaaa gaaaaacagt aataactaga ggtgattaag atggaagata tgacgagaat
    31561 tcctcctcat agtgtagaat cagaacaatc aatattgggt tctatacttc tagataaaga
    31621 tgctataata acggttacag aaactataaa gcctgatgat ttctataaag aggctcacaa
    31681 aataatatat gaatgtatga taactttaag taataaaggt gagcctatag acttgataac
    31741 attaacagaa gaactaagaa aacaagggca tcttaatgat attggaggaa tcagttatat
    31801 tacaagtctt tctactatag taccgacgac ttcaaatgta aaatactatg ctgatatagt
    31861 aaaagaaaag tctgtactta gaaagcttat aaaagcttct aatgaaataa taaatctagg
    31921 atatagtgga gcaactaaga ttgaagatgt tttagaacaa gctgaaaaga gtatatttga
    31981 tatatcacag gagaaaacta gtgatgattt taaatctata aacttagttt tgatggatgc
    32041 ctatgatatg atagaaaaat tatacactaa caagagtgat gtcactggta taactactgg
    32101 ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg actgatttaa tattaattgc
    32161 agctagacca gcaatgggta aaactgcctt ttctttaaac ttggttcaaa atgcagcctt
    32221 aaaaggtgat gcctctgtag ctgtatttag tttagagatg tctaaggaac aattagtaca
    32281 acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa ataaagactg gaacacttaa
    32341 tgataatgat tggcctagaa ttatagatgc tatggcagta ttatcagatg ccaaaataca
    32401 catagatgat actcctggta taaaaatatc agaactaagg tcaaagtgta gaaagttaaa
    32461 aatagaaaaa ggactagatt tagtgcttat tgattatctt caacttatgg aaggtgaagg
    32521 taataatgaa agcagacaac aagagatatc taagatatca aggtcactaa aaatattagc
    32581 aaaagaatta aactgtcctg tagtagcact atctcagtta tcacgtgcac cagaacaaag
    32641 agcagaccat agacctatgt tatctgattt aagagagtct ggagcaatag aacaggatgc
    32701 agatatagtt atgttcttat acagggacga atattatcat gctgattctg agagtaaaaa
    32761 tataggagaa gtaataatag caaaaaatag acacggtgaa actggttctg tggaacttgt
    32821 ttggcttggt gaagttcaaa gatttgggga taagttgaga gacctataat ctaacgaata
    32881 tccacagaac gtcctttctt tctacaggaa aaatgtggat acatgtcact aaatatgtcc
    32941 aaacattgaa atttagctaa aaaactatcc acactatcca cagatggcct gttgataagt
    33001 gtgaataagt agataaagca agcttttggc ttgctttatc tgtgtataaa tattaattat
    33061 taggaggaat aatatggaaa aaataagctt aaaatatata tatcccaata taattaaagt
    33121 acttgatgaa ataaacttat ttagagtaat tgacaataat ttaagagaat ctattgttgt
    33181 ttatgctaat aatgtggata atcagtatca tataaacatg actaatacaa actttggaaa
    33241 cataattaac atatgtaaat tagaaaaact tttagatgtg gataaattta tggaaaaagt
    33301 tataaaatat gaaaaagaaa taatagagaa agaagagttt tcaaaaatag aagaatatat
    33361 gttaaatatt ggagaatact aaaaaagttg aaaaaaatac gaatgtttga tagaataagt
    33421 aagtaaagtg ttcgagagta tataaaatat agaggtgaat tacatgaaaa cagttgcaat
    33481 tgttggttct caatggggag atgaaggtaa aggtaaagtt atagattatc ttgctactca
    33541 agcagatgta gtagtaagag ggcaaggtgg aaataatgca ggacatacat tagttgtaga
    33601 aggtaaaaaa tacgcattac atctaattcc atctggagtt ttaaatccaa atacagtgaa
    33661 tataatcgga aatggaatag tgtttgaccc taaaggattt ttagaagaat tggaaatgtt
    33721 taaaacggat aatataagta cagaaaatat aaaaataagt gatagagcac atgtcatatt
    33781 cccatatcat aaagaactag atgcattgtc agaagaagcc agaggcgatt taaagatagg
    33841 tacaacaaaa aaaggtattg gaccttgtta tatggataaa acagagagat ctggtattag
    33901 aatatgtgac ttaatggata aagataaatt tgctataaaa ttaaaagctc aaatagatgc
    33961 taaaaatgaa atagttaaaa atatatatgg aaaagaagaa ctatttgatt ttgaaactat
    34021 atataacgaa tatctaggat atgcagaaca aataagaaaa tatgtggcag atacgtctgt
    34081 tattgtatat gatgcagtaa gagcaggtaa aaaagtattg tttgaaggag cacaagggac
    34141 tttattagat ttagatttag gaacttatcc atttgttaca tcttctcatc caacatctgg
    34201 tggatttgca attggtgcag gtataggacc aaatatgata aaagacgttg ttggtatagt
    34261 aaaagcatat actacaagag ttggagaagg accgtttgtt acagagcaaa taaatgaaac
    34321 tggagataag ataagagaac aaggacatga atttggggta actactggta gaccaagaag
    34381 atgtgggtgg tttgatgcag ttatagttaa gtatgcagct agagttaatg gattgactag
    34441 tatttcattt atgttacttg atgtattaac tggatttgat aagataaaag tttgtacgtc
    34501 ttataaaatg ggcgataaga taataacgga tttcccagct tcattagatg atttagcaaa
    34561 atgtgaacct gtatatgaag aattagatgg atggaatgaa gatataactc aaatagataa
    34621 ctttgatgac cttccagaga atgcaaagaa atatgtagca aaaatagaag agctagttgg
    34681 agtaagtgta gatatggttt ctgttggtcc aaatagagct caaactataa taagaagaaa
    34741 tatatttgct taatatttta tcaattaaat aatttaaaat agttgaagaa gagtatctca
    34801 agataatgtt tatagttatt agagatgctc ttttatattt gacctacatt gattttttat
    34861 taagttaaag ctacttatac atattctaac tgaaattaaa gttgaattta tgtagataca
    34921 tataggtttt ttaaaaagaa ttttgttgaa aataaatggc taaagatgat tttaattata
    34981 ttctaaaaaa agtaaaaaaa actattgact aaaaattata tagacgctat aatataactt
    35041 gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg tctattagct cagtcggtag
    35101 agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaatat
    35161 ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc
    35221 ccctacgagt caccagtatg ggactatagc tcagctggga gagcacctgc cttacaagca
    35281 gggggtcaca ggttcgagcc ctgttagtcc caccaaattt tgaattagag ataaatacta
    35341 gattatatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc aggttgtagg
    35401 ttcgattcct atcaccagct ccacaaagta cataaattca aattaatata attttctatg
    35461 ttacaggata cttttaagta tcttttttta atttcatttt tattgtatta aaaatgaaat
    35521 taaacataca acaaaatttt gaattacttg caatgtgata gttttaaata taaaatttag
    35581 aatcattcta gcatagtatt atgtaatata gcaaatttaa tttatagtat caataaatat
    35641 agactactaa gaatgtaaat gatttgttag attaagatac attagatatg taaatagaaa
    35701 aataaaaagt atgttttaat ttatgtgata taagttttaa acagagaagc aagtttgctc
    35761 ttacctctct atttaaaaat aaaaaaatgt tttttaaggg ctattttatt taaatctttc
    35821 ccatctcaaa tcatctccag taaattctaa aatcctaaac ttatctaata ttctagagag
    35881 agttctttga gtgtaagtat ttcctatttg agatggtgtt aagttggttg aaataatgat
    35941 ttttttacca gcaacaagtc ttgtatttac aatattgaag atttctccac tagtaaaaga
    36001 attattaagt tcagttccaa ggtcatctat tattaataaa tcacaatcaa acaagttttt
    36061 ataattatct tcacttattt gattatttgt gtctcttcta aatttataat cttcaagaat
    36121 atctaaaatt ctaaaagaag tttgatagat tacaacattg cctttatcta acaattcctt
    36181 tgcaatacaa ttgcacatat aggtctttcc tagtccagta gaaccataaa acagtagatt
    36241 ttcactatta tccttcttaa aatcatgaac aaaattttcg cagatactta aattatttag
    36301 catattttct ctaggagata tttcaccatc agagcctttc ttaggagaaa atatattcaa
    36361 gttaaaattc gaaaaatttt cctgacttaa aatcctatct aaattagaca ttttataagc
    36421 ttcatttaca atttcttgtt tgaggcaaga acatttttct ccattaggta aaaatccttt
    36481 atctttacaa gaaatacatt gaaactttat ttccaagtaa tcaagaggta tgttactttc
    36541 agctaaaagt ctttctttct ttacttttaa agactctata ttctctttag tcttctttac
    36601 aatttcatct ttagatttgg gatttaaaag tactatctta gctaaactga gacctatttt
    36661 agaaatttca tcatctatag attttatctc cgggattcta ttatatacct cattttttct
    36721 atgttctaat aagagttcat tatcatctct tctcttagca tatttagcaa gtatttttct
    36781 tattttatcc tcattcattt aatcacctac ttaaactttt ctttttgact ttttttaata
    36841 atctcatcga gctcatcaga ggtatactga gtgaatgttt cgttgaaatt atgaaactta
    36901 gtttttttgt aagtattgtt gttttgagta gtattaatct gcttcttatt aatattctcc
    36961 ttaactatac gttcttcctc tttttgcttt aaatcattta aattttttat atttttttca
    37021 ttccaatttt taattatacc atttatataa gatatacttg ggtttgaaat attcttagac
    37081 ttagaacatg catttattat caaatctata tccatattaa atttatcaaa ccaggtgtcc
    37141 ataagttctt tttctgattt tgatggttgt cttgaaaatc ccaattcatt gaaaatcgtt
    37201 ttatataaaa tatatcgttc acttcttacc aaaaagcttt cttcaacatc ttttggtgta
    37261 tataaatttg aatcatacca atttctaata atgccttcaa tgtatttaac gggctttgat
    37321 gtaccagttt tatctttgac gtattcatat gcgcaaagta tcatatcggg gctcatattg
    37381 tatttattca ttatatccat aatactcatc ttttcacttg ggtcgagata tcgcccaact
    37441 attttattta tagaattaaa catttttcta atacttggat tttcagaagt agatacaact
    37501 ttatctgtat tagattttat agatgaatta ttgcttaata tattctcaat atagaaattt
    37561 tttaaatcta aaaattctat tgaataatta aaattatcat attctccatt gtcatgtatt
    37621 tttataatct tcttttcttc ccagaatttc caagcagata acacatcaga caatggtata
    37681 tttaggtttt tggctattga attattgtcg aacttaggat tagaagtaat atcacatgct
    37741 tgtctatatc ctaaaagata aacttttacg tataacccgt ccgccattgg cataaatata
    37801 tctataaaga tattgggaat agtgatttca cctaaatcta tttcattgct ttctttaaaa
    37861 aacattttat cacctcagat taaattatat cattttttat attatatttg tctgtaaaca
    37921 aataaaaggt cacaagatta gcatgtaaat attttctaaa agtattcatt ttgaaaagga
    37981 ggagatattt tggttataaa attcaaccaa aaagtaagaa gaacattatc agtagcgatt
    38041 ctatttatgg tattaataat aggttgcttt atagaaaagg atgacagttt agaaacatac
    38101 aaagaaaaaa ctaattcaaa aataaatcag tataatatgg atgttatatt tgatgatgag
    38161 actaagaggt taatgtgcaa tcagaatgtt gactacataa ataatacaaa atcaaacata
    38221 gataaaatat atttccatat atatccgaat gctttttcca agaaagattt tgctcctttt
    38281 gaaaaaagtg aaatgacaag ggcttatcct aatggattta acgaaggata tatagatata
    38341 aagaatatat taaataataa tagtaaaatg gagtataaaa ttaaaggaga caaaaatgac
    38401 atattagaaa tagaattagg gaaagagtta aaaccaaatg aaagaatgtc attagatata
    38461 aaatataacg taaaaatacc aaattcaatt ggaagatttg gatatggaga aaatactata
    38521 aatgttacta attggtttcc tatagcttgt gtaaatgatg aaaggggatg gaatttaaag
    38581 agttatgaaa ctattggcga cccattttat agtgagacta gcgattttca tgtgaaacta
    38641 ttgattccaa ataaatataa aattgctcat acaggaaaac ttattgatga taaacatgat
    38701 aataagaaaa tgctatatga aatagaagct gaaaacgtta gagattttgc atttatacta
    38761 agtagcaaat ttgatgtaaa caaagtagat tcaaagggag tagctatcaa tacatataat
    38821 ttaaataaaa agctttctaa aaaagcaact gaagtggcaa aagattcaat aaatatattt
    38881 agtgaattat ttggaaagta tccttacaaa gaattttctg ttgtagcaag tgactttttt
    38941 atagggggga tggaataccc tatgcttgtt atgatagacc aaagtttata taatgaaaaa
    39001 aatgaatttt tactagaata tgttattgcc catgagacag cccatcaatg gtggtattct
    39061 gctgttggaa atgatgaaat aagtgaaccc tggttagatg aagcacttac tgaatattct
    39121 acaatagttt attttgaaca aaagtatggt aaagatatgg caaacaagct tataaaaact
    39181 atggagatac agacaaaaag ttatttgagc gaaaatatat ttaagccagc aaatgagtat
    39241 aaaaattcta ctgaatacag cttgaatgtt tatacaaaag gcgcaatagc atttaatgaa
    39301 ataagaaaag aagttggaga taaagtattc tttgagactt taaatgagta ctataataaa
    39361 tataagcata aaaatgctaa tggaagagct tttgtggaac tatggaatag taaaggtgta
    39421 gatataaata agattataag tgaatgtaag taaacagtga atgtaagtaa attttgtgca
    39481 gagtattaaa ttttatttaa tactctgttt ttttggtata atataattta taaggcagaa
    39541 aaagaggtga aggaatgcta aagtattgtt cagtagggag tggtagtagt ggtaattgcc
    39601 actatatagg gtataaaaat actaatatac tagtagatgc aggattaagt ggcaagagaa
    39661 taactacagg tcttaaagac attggtgtag atgcagataa attaaaagga atatttataa
    39721 cacatgagca tgttgaccat ataaaaggag caggtatatt atcaagaaag tttgatatac
    39781 cagtatttgc taatataaaa acatggtgtt ctatgaagga caaattaggt gacatcaaag
    39841 ataaaaatat gaaagtattt gaaaatgata aaacctactc attaggtgat atcatagtaa
    39901 gacctttttc aatagaacat gatgcgtctg acccagttgg atataatttt tatacagaga
    39961 gtgatgaaaa aatgtctata gctactgata taggatgtat aactcaaaat ataaaaaaac
    40021 atctatataa atctaaatta gtagtattgg aatcaaatta tgaccctaat atgcttatga
    40081 tgggttcata tacatatgct ttaaagaaaa gagtaatgtc aagtactggt catttatcca
    40141 atgaagatgc agctaatttc tgcgttgaac ttattaatga aggtacagaa tctatattat
    40201 tagcacattt aagtaaagaa aataattttc ctgagttagc gtatgagact tcaaaaggag
    40261 tattagcatc aaatgatata gtagttggac aagatgtaaa actagatgta ctatcacgaa
    40321 atgatgtttc taatgtttat gagatgaagt agaggtgaat aactttgcta aagagaaatt
    40381 ttgaaaatgt tgaggataat aaaattttat actctataaa gcagggtatg attttagcga
    40441 tacctgctat tatgactggt tcaactgctc ttgtaatttt gaatcttcca ataaaggctt
    40501 atcaagaata tttatcaggc ttgtttaatg gagaggttac aaatattctt aactttataa
    40561 atgattctac tttaggaatt atttcattaa taatattatt aacaataagc tatagttatg
    40621 gaaaaatata tgggagcaag tatacagtac ttgttcctat agttgcaatg tgctcttttt
    40681 tagtattttc acatggtaat gagcttaatt cttatataga aatatttaaa acaaagtggt
    40741 tatttacatc tataattgtt tctatgacat cttctgtatt gtttgtaaaa cttacagaat
    40801 cttttgttac aagtgtaaag tttcatacag aaggggctga tgcagacttt aatatggtag
    40861 tttcggctat agttcctttc ataatagtag tatttttgtt ttctattttt agagtgataa
    40921 tgataagtct aattggcagt tcaaactttc aagatatatt ttataattta ttttcaaatg
    40981 tttttaataa aatgggaaca aaccttatga gtgctctgtt gtttattttt ttaatgcact
    41041 ttatgtggtt ttttgggatt catggtagta atgtattaga taccgtcgca aaaaatttat
    41101 ttgaaaatag tatggcaatt aacataaatt tagttaataa taatcaatta ccaacagaaa
    41161 tttttactaa gacctttttt gatacaatgg ttttattggg aggatgtggc tctttactat
    41221 gtttagtaat tgcaattttt cttagtgaaa aacgtataaa tgtaagaaag ttagctaaaa
    41281 ttgcttcaat accagctctt tttaatatca atgagatgct agttttcggg ataccaattg
    41341 tttttaacta tattatgttt gtaccttttg taattactcc tataatctta accattacaa
    41401 gttatgtggc tatgtctaca ggtattgtac catgtacagt ttcagctgta gaatggacct
    41461 cacctatatt tttaagtgga tatatgtcta caggttcaat aagaggtagt ttgttgcaag
    41521 tatttaattt atgtgttggt gttatgattt atattccgtt tataaaaatg tcgcaaaata
    41581 gatatacata tatgtttaaa aataatatag aaaatttaac aaacttatta aaaaggtatg
    41641 aattaacagg tgaacagcct aatcttctaa accataatgg taaaataggt tcaatttcaa
    41701 aaatgttggc atcagacttg aagtttgcta tgaaaagagg agaaatagag cttttttatc
    41761 aaccacaagt caattataat ggaaatgttg tgggagcaga gggattactt agatggaaac
    41821 attcaatagg agggttcatt tatccaccac tagttataat tttggctaaa gaagaagggt
    41881 tcttagatga gcttggagag tatattatta ataaagcatg tattgattta aagaagagtg
    41941 aaaagctatt gagaaatcct gttaagttct ctgtaaatat atctccagac caacttgatg
    42001 accctaagtt accagagcaa ataaaaaata tcattagtag taatgatata aatcctaata
    42061 tgttaggtat agaaataaca gagcaaattg cattatcagg ttcacaaata attattgaac
    42121 gtatcaatgc gatacataac ttgggaataa agttaattat ggatgatttt gggatggggc
    42181 atagctctct tatatatctt caaaataata actttgatat agttaaattg gatggttcat
    42241 tagttaaaga gatacttaca aataaaagaa gtagtgaaat tataatgact attgtaaatc
    42301 tttcaaaaaa tttagatttt gatatagttg ttgagtatgt tgaaaatata gaccaaaggg
    42361 ataagttata tggtttagga tgtgaaatat atcaaggata ttattatagc aaagcaatac
    42421 cttttgaaga gtttgtagaa tatgcaaatc aagaaagaga gatataattt tataagagct
    42481 tgtaaattct agtaatgcat agatttgtaa taaaattaag tttaggagaa atatatgaat
    42541 ataagtatag tagtagtagg taaaattaaa gaaaaatatt taaaattagg tattgatgag
    42601 ttcaagaaaa ggctttctaa gtattgtaag ttagaaatta tagagttgga tgatgaaaaa
    42661 gctccagaga atttgagtgt taaggaaatg gaaattataa aagacaaaga aggaaagaaa
    42721 attttaggga agataaagca taatagttat gttatagcct tggctattga tgggaagggt
    42781 ttatcctctg aagaacttgc taagactatg agtgatttag cagttagagg taatagtagt
    42841 ctctgtttta taataggagg ttctctaggg ctttctgatg aggttttagg aagggctgat
    42901 tataggttat ctttttctag gatgactttt cctcatcaga tgatgagatt gatattgtta
    42961 gaacagattt atagggctta taggataaat aatggagaac cgtatcataa ataaatgata
    43021 cggtttttgt tagtttgcaa cttaataata ttaaaataac aaaaaagtat tgaaagtata
    43081 ttgcatttaa ggtagaatta aataaaatat tataacatta taatgtttat atggttgtta
    43141 ataaaaattt ggaggaaaat atatgataaa aataatgttg gcctgttcag caggaatgtc
    43201 tacaagttta ttagttacta aaatggaaag tgcagctaaa gaaaatggta tagagtcgca
    43261 aatttgggca ataccagaat caactataca aaatgaaata gaaaaatgtg atgtattatt
    43321 actaggacca caagttagat atgtattacc taaggctcaa gaaattgcta agccatataa
    43381 tataccagta gaagttataa atatgatgca ctatggaaca gttaatggag aagctgtttt
    43441 aaatagagcg atagaactta acaacactaa gtaatataaa ttataatgta tttttaatat
    43501 ataaattggg agaatcaaag gatggatgaa aagattatag aaatatcttt taatataata
    43561 ggttatgcag gtgaaggtaa aggattagct tttgaagcaa taaaagaggc taagaatgga
    43621 aatatagaaa aagcaaaaga attattaaaa gagtctaggg aagttataaa taaagcacat
    43681 agatatcaaa cagaattaat acaaaacgaa gctagtggaa acaggactga aataagtgtc
    43741 atacttatac atgcacagga ccacctaatg aatggcatga atttttaaca gttagctgag
    43801 gaaatcgttg atttacattt gaaattacaa gataaataaa aattaaagag aataaattaa
    43861 gagagttctt aaaagataca ttatgtgtag cacactaatt gttagattat tgattcaata
    43921 tttagtgagt aatacattag gattattttg aggactcttt ttat
//
LOCUS       DAENCV010000022        42900 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAENCV010000022 DAENCV010000000
VERSION     DAENCV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.022"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            446..3994
                     /locus_tag="KQD82_001132"
     CDS             446..3994
                     /locus_tag="KQD82_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4379610.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRWFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4179..6113
                     /locus_tag="KQD82_001133"
     CDS             4179..6113
                     /locus_tag="KQD82_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG4379611.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6219..7214
                     /locus_tag="KQD82_001134"
     CDS             6219..7214
                     /locus_tag="KQD82_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4379612.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7186..7971
                     /locus_tag="KQD82_001135"
     CDS             7186..7971
                     /locus_tag="KQD82_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG4379613.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7959..8657
                     /locus_tag="KQD82_001136"
     CDS             7959..8657
                     /locus_tag="KQD82_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379614.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8780..9253
                     /gene="ybaK"
                     /locus_tag="KQD82_001137"
     CDS             8780..9253
                     /gene="ybaK"
                     /locus_tag="KQD82_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG4379615.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9450..10289
                     /locus_tag="KQD82_001138"
     CDS             9450..10289
                     /locus_tag="KQD82_001138"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG4379616.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10302..10850
                     /locus_tag="KQD82_001139"
     CDS             10302..10850
                     /locus_tag="KQD82_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG4379617.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10916..12097
                     /locus_tag="KQD82_001140"
     CDS             10916..12097
                     /locus_tag="KQD82_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG4379618.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12392..12778
                     /locus_tag="KQD82_001141"
     CDS             12392..12778
                     /locus_tag="KQD82_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379619.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYNMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12928..13191
                     /locus_tag="KQD82_001142"
     CDS             12928..13191
                     /locus_tag="KQD82_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4379620.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13320..14162)
                     /locus_tag="KQD82_001143"
     CDS             complement(13320..14162)
                     /locus_tag="KQD82_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG4379621.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14457..15191
                     /locus_tag="KQD82_001144"
     CDS             14457..15191
                     /locus_tag="KQD82_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4379622.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15201..16334
                     /gene="nagA"
                     /locus_tag="KQD82_001145"
     CDS             15201..16334
                     /gene="nagA"
                     /locus_tag="KQD82_001145"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG4379623.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAKVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16433..17182
                     /gene="nagB"
                     /locus_tag="KQD82_001146"
     CDS             16433..17182
                     /gene="nagB"
                     /locus_tag="KQD82_001146"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG4379624.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17357..17629
                     /locus_tag="KQD82_001147"
     CDS             17357..17629
                     /locus_tag="KQD82_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379625.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18289..18993
                     /locus_tag="KQD82_001148"
     CDS             18289..18993
                     /locus_tag="KQD82_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379626.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18995..20482
                     /locus_tag="KQD82_001149"
     CDS             18995..20482
                     /locus_tag="KQD82_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379627.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20687..22300
                     /locus_tag="KQD82_001150"
     CDS             20687..22300
                     /locus_tag="KQD82_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4379628.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22663..23493)
                     /locus_tag="KQD82_001151"
     CDS             complement(22663..23493)
                     /locus_tag="KQD82_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4379629.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23565..25292
                     /locus_tag="KQD82_001152"
     CDS             23565..25292
                     /locus_tag="KQD82_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379630.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25329..27191
                     /locus_tag="KQD82_001153"
     CDS             25329..27191
                     /locus_tag="KQD82_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379631.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27524..28213
                     /locus_tag="KQD82_001154"
     CDS             27524..28213
                     /locus_tag="KQD82_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG4379632.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDDNMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28318..29004
                     /locus_tag="KQD82_001155"
     CDS             28318..29004
                     /locus_tag="KQD82_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG4379633.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            29021..30928
                     /locus_tag="KQD82_001156"
     CDS             29021..30928
                     /locus_tag="KQD82_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG4379634.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31381..32070
                     /locus_tag="KQD82_001157"
     CDS             31381..32070
                     /locus_tag="KQD82_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379635.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32506..33045
                     /locus_tag="KQD82_001158"
     CDS             32506..33045
                     /locus_tag="KQD82_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379636.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            33064..34107
                     /locus_tag="KQD82_001159"
     CDS             33064..34107
                     /locus_tag="KQD82_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379637.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            34109..34945
                     /locus_tag="KQD82_001160"
     CDS             34109..34945
                     /locus_tag="KQD82_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379638.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            35050..35724
                     /locus_tag="KQD82_001161"
     CDS             35050..35724
                     /locus_tag="KQD82_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379639.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35724..36776
                     /locus_tag="KQD82_001162"
     CDS             35724..36776
                     /locus_tag="KQD82_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4379640.1"
                     /translation="MIKFKKAISLIAVGMMIVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            37006..37854
                     /locus_tag="KQD82_001163"
     CDS             37006..37854
                     /locus_tag="KQD82_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4379641.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37844..39508
                     /locus_tag="KQD82_001164"
     CDS             37844..39508
                     /locus_tag="KQD82_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4379642.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39557..40813
                     /locus_tag="KQD82_001165"
     CDS             39557..40813
                     /locus_tag="KQD82_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379643.1"
                     /translation="MRDLNVSEYLFIFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40836..>42900
                     /locus_tag="KQD82_001166"
     CDS             40836..>42900
                     /locus_tag="KQD82_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG4379644.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIK"
BASE COUNT        17049 a         4353 c         7417 g        14081 t
ORIGIN      
        1 tattatctgg taacttaagt tttgagtatt aatctaaagt atatgttttt atatggttta
       61 aattttatgt atacaaaatt aatttgctaa ctgaatcata taaaaaaaaa caaaatattt
      121 tttagaggga atgattagat agaatttttt tgttatgaaa ttaattgaag agtaattaat
      181 tagaaaagga atgaataata atgaatatat tacattatta ctttttgata aatcaaaaat
      241 aaagttataa tttttaatat taattaagaa tgatgaaata tgaataaaga ttttacaaat
      301 aaatcttaag ctatcttatt taattctaat acaggaagaa ggtaaatgta tattgaaaaa
      361 atcatagaaa tagaagtaaa caatttaaat aaatacaaag gtttgcaaaa gaagtaatat
      421 aattatatac aatgaggatg tgagtatgat gaacagacta aagccaagaa ttttaggtat
      481 tagtatcttg tttattatta taattatttt aacatgtgga gctgttttca agtttaaatt
      541 taaattagat aataaattaa aagaaaccac acatacatat ttgtctgagt ttacagaaca
      601 caatataatt aatctcaaga caagacttag tggtcaattt gacatgcttg aatctattgc
      661 taacttcata ggaaacttga ataatataaa tgatgaaatg gtgattgatt taatggattc
      721 tgatgtaaag cgaagttctt taattagaat gactatctct acattagatg gggaatgtta
      781 ttctaatgat ggaattaccc ataatgttca agatagagat tattttaaaa aagctaaaaa
      841 tggagaaaag aatatatcag agcctttaaa atctattgtt aataataaag aaataatagt
      901 tttatctacc ccagtttata aaagtgggaa aatagttggt gttttaagtg gaatttatga
      961 ttcaaaaaag ttaaatgagt tattaggatt atctgcattt gatgaaaaag cagatgtttt
     1021 tgttgcaaag aaaaatggag atatagttgc tcaaaaaaca aataaaaata gaaagacgct
     1081 atcatgtata tttcagcttt ttgacaaaga taactgtgat gaagatgatt gtctaaaaac
     1141 tataaaagaa aatatgaaaa atgataagtc tggatatttt aattataact tagataatga
     1201 aaaatttatg ttaaactatc agccacttgg agttaatgat tggtatgttt tttctgttgt
     1261 gccaaataaa atagtatcta ttcagtcaga gaatatatct tatgatgtat atatttttgc
     1321 attagaaata ctactagtta tattgatttt tataatatat atagcatgta ttataaataa
     1381 taacaccaaa attattataa atgaaaaaga aaaatttaaa gtactcactg ataatatatt
     1441 aggaggggtt aaaacctgtt taaaagatga atatatgact atgacatatg taagtcaggg
     1501 gtttttagag cttacaggtt actcttgtga agatttaaat aaaatattta aaaatagatt
     1561 taaagacatg atttataaag atgatttaga caagtttaat tgtattattc taaatcaatt
     1621 agaaaaacaa gataaaattg aagttgaata tagaattgta agaaaagatg gaagtataat
     1681 ttggattttg gagagaagta agtctataaa tgataataaa ggaagtgagt gtattcaggg
     1741 agttttaact gatataacag aacttaagaa aatacaagaa gaattagatt caaaaagatt
     1801 tgaaatacag gctatcaatg atagtattgt tggtggaata gtgataactg agataaatga
     1861 tgattttgat agtatatatt taaatgaagg ttatttaaat atgattggat atacacgcaa
     1921 acaactggaa gatgagctaa ataataaatt aatcaaactt atttttaaag atgatagaac
     1981 agaattaatt aatagtataa acaaacaact caaattaagt gatacatttg catctgaaca
     2041 aagagttgaa aaaagggatg gcactattat atggatttta ttaaaaggaa gaaagatgat
     2101 ttatgataat ggaaaatctg tactttgttg tgttattgta gatattactt ctgctaaatt
     2161 attagagcaa gaacttaaaa agagtgaaga acgtcatgaa atcattttaa agcagacgca
     2221 aaatattata atagaatggg atatagaaaa agataaaatg aatatatcac ctaattggta
     2281 taataagttt aaatatgacc ctataactga aaacgcaaca aaaaatataa ttaaatcagt
     2341 agatatatat catgatgata tggataagtt tatttcatta attaaactta cttcacaagg
     2401 tagtgagtat gaagagtgtg aaattagact tagaactaga gatactattt acgcttggtt
     2461 tagtataaag gcaacaactt tatttgataa taatggtaaa ccttataaag tagtaggaag
     2521 tttgactgat attgatgaat caaaacgaga agctgaaaag ttgaaagaaa gggcagaaaa
     2581 agaccctctt acagatttgt ataataaaaa agcatcacaa tcacttatag aagagtacat
     2641 aacattggat aaaaaaagaa aaggtgcttt aatgattatt gatattgatg actttaaagg
     2701 aataaatgat aatttaggac atttatatgg ggatgctgta ttaagtgaaa ttgcaaatga
     2761 cctaataaat ctatttagag caaccgatat agtaggaaga attggtggag atgagtttat
     2821 tgtatttata aaagatattt ctgaaacaag agatattata aataaagcag aagaattggt
     2881 aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat tataaaatat cattaagtgt
     2941 aggaattgct aagtttccac aagatggtat ggactatatg acactattta gaaatgcgga
     3001 tatggctctt tactcagcca aagggaaggg taagaattgt tatacattgt atgataaaaa
     3061 aattgataaa gtccaatata taactaatac aagaaatgca ggagatagtg ataatgtaga
     3121 gatgtctttt aaagaacata tcacaaatta tatatttgat atactatatg aatcaagtga
     3181 tatagaagtg gcagtaaatt taattctaag tatcgtaggt aaacacttta atgtgagtcg
     3241 catgtatata tttgaaaatt cagatgataa tcgagtatgt aaaaatacat ttgaatggtg
     3301 taacgaaggt gtaacctcgg aaattaataa tcttaaaaat atttgttaca aggatttagg
     3361 caactatctt ttaaatttta atgcagatgg gttattttat tgtaatgata ttgaaaattt
     3421 aggtagtgat gtgaaaaaag tacttaagcc acaaggcata cgttcaatat tacaatgtaa
     3481 aattttagag ggtaatgaat ttagatggtt cataggattt gatgaatgtt catataatcg
     3541 tatgtggact aatgatgaag tgaacacttt gacatttata tctaaaacgc tcagtatgtt
     3601 tttattaaaa atgcgtattc agagtaagct agaagagtct ttaacaattc aaaaatctgt
     3661 gatggataat atggatatat gggcatatgt tgttgacagg aatacctatg aattactatt
     3721 tatcaataaa aaaatgcttg agtcagcacc aaacactcat gtaggtgatt tatgttataa
     3781 agcgatatgg aataatcaag ataaaccttg taaaaaatgc cctatgatat atttaaaaga
     3841 tggtgatgat acttataaat cattcatgca taataaaaag tttgaggtac aggcaaatat
     3901 aactacaact actttgaaat ggtttgatgg taaagatgca tgtttaatat ttggaagtga
     3961 aataactagt attgaagctg aagaaaatac ttaaaatact gtgttatata tatacttaaa
     4021 atcgcaattt gaatttatta gacttaaaat gtctatataa atcatttaag ttgtggtttt
     4081 aagtattttt tatgatataa ttaagcgtta agaacaaaat taaaagatac ttttgtataa
     4141 aaaaatattt gttaaatata tattgagcag gtgataatat ggaaaataag tttttaccaa
     4201 taagcaaaca agatatgata gatagaggat gggaagaatt agattttgtt ctagttacag
     4261 gagatgctta tgtggaccat catagttttg gaacagcaat aatatctaga gttttagaaa
     4321 gtgctgggta taaagttgga ataatagctc aaccagactg gagaactaca gatgatttta
     4381 tgaagctagg aaaacctaga cttgcatttc tagttaatgc aggaaatatg gattcaatgg
     4441 tcaatcatta ttctgtaagt aaaaagcata gagaaaagga tatgtattct ccaggtggga
     4501 aaatgggtta tagaccagat agagctacta tagtatattg caataaaata agagaagcat
     4561 acagtgatgt tgcaatagta ataggtggaa ttgaagcgag cttaagaaga tttgcacact
     4621 atgattattg gagtgataag gttagaaagt ctatgttaat agatagtgga gcagatttat
     4681 tagtttatgg tatgagtgaa aagcagattg tagaagtagc caatgcactt aatgatggat
     4741 atgacccaaa atacattaga catattgatg ggacatgcta tatttcagat acattggaag
     4801 aaatatatga taaatatata ttaattccat catataaaga aatttgtgaa gataaaatga
     4861 agtatgtaga agcttttaaa attcaatatg atgagcaaga cccatttaga ggtaatataa
     4921 ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa gccagaaaaa ccacttagta
     4981 gagaagaact agatgaagta tatggattac catatcagaa gacataccat cctgtgtatg
     5041 aaaaatttgg aggaatacca gctatagaag aagtaaaatt tagtatagta agttcaagag
     5101 gctgttttgg tagttgttca ttttgtgcaa taacatttca tcaaggtaga gcagtgcaaa
     5161 gtagaagtga aaaatcaatt attgatgaag caataggaat aacaaattta gatgatttta
     5221 aagggtatat acatgatgtt ggaggtccaa cagctaattt tagaagacca gcatgtaaaa
     5281 aacaaattac taaaggtgct tgtaaaaata gacaatgctt atctccatca ccatgtaaaa
     5341 atttagatgc agaccacagt gaatacttac atttacttag agctgtaaga aaattaccta
     5401 aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga ttatgtaatg gcagataaga
     5461 acaataaatt tcttagagag ttgatagagc atcatgtaag tggtcagtta aaggtagccc
     5521 cagaacatat atcagaagaa gttttaaagt atatgcaaaa accagcagga aaaacttatg
     5581 ataaatttag acagaagttt tttgctataa atgaagaatt aggtaaaaag caatatttga
     5641 taccctattt aatgtctagt caccctggtt ctaccttaaa tagcgctata gagttagcag
     5701 agtatttaag agatactcac taccaaccag agcaggttca ggatttttat ccaactccag
     5761 ggactctatc tacaaccatg ttttatacag gtattgaccc gcttacaatg aagccagttt
     5821 atgttcctaa atcaaaacgt gacaaggcga tgcaaagggc tttattacaa tatagagcac
     5881 ctagaaatta tgatttggta tactctgcgt tagttgaagc aggaagagaa gacttaattg
     5941 gttttggaca tagatgtctt ataaagccaa aaaatgaaaa gccttatttt aatagaaata
     6001 attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa aaaaactaat acaaataaca
     6061 gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa gaaaaaacat taaagtataa
     6121 ataaaaatac ttaaaaaaga aggattattc gataaattaa cagaaattag tataaataaa
     6181 tataattatt ttaatgacgt tacctttggg aggaagagat gaaaaagtat atttctataa
     6241 tattattagt ggtgttgact atggttgttt tggtaggatg ttcaccagga aaagataacc
     6301 ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt agctgttcca gatggactac
     6361 cagcagtggc aatagctaaa cttgcaaatg aaaatccaga gattaaagaa ggatatgaaa
     6421 cagtgtatag cattgaaaag acacctgaag cgatttccac tagagtgatg aaaaaagatg
     6481 cagatatagc tatagttcct tcaaacatgg ctgcaatagc gtataataaa acttcatcat
     6541 ataatattgt tggaactgta ggaatgggtt cactttattt ggtgtcaaca gaaaatataa
     6601 aagattattc tgatttagtt ggtaaagaag ttggatgtac tggaaaagga cttactccag
     6661 acataactat aaaaagtctt ttgttacaaa aggatataaa ttattcagat ataaaattta
     6721 attatgtaaa ttcagcaagt gaactggtac cattattggc tactggtaaa gttaaaacag
     6781 gtattgttcc agaacctgca cttagtgctt tgatgtctaa aaatccagac ataaaaatta
     6841 taaaaagttt aaatgactca tggaaggaag taagtggttc aaaagatggt tatcctcaat
     6901 ctactttaat tgtaaagtct gaatttttaa gagataataa agattttgtt gattcttttg
     6961 taggtcaatt atcaaatagt atagattggg caaacaaaaa tcctgaagaa ttaggtgcat
     7021 attctgaaaa aataaaaata tctactgaat caaagataat aggaaaatct cttgaaagag
     7081 ctaatttaaa atatattcca gttaaggaca tgattaaaga ttataaaaat tactatgaga
     7141 aattagctaa ttttgatgat aagacattag gaggcaaagt accagatgaa gcaatatact
     7201 ttgttgagaa ataaaaaaaa taaagaacaa tttatagata aaatggaggt aacactttct
     7261 tgtgttatcc tcttattttt atggcaaatt attgctttaa aaattgataa tgatattttt
     7321 ttaccaacag taggtcaagt atttaatagt ataaaagaga ttatgctaaa ttctagtttc
     7381 tatatagata tactttactc tatggggaga tgttttttta gttttttact ggctatgatt
     7441 tttgctatta tactttcaat agtgtcttat ttaaatagat tttttagaaa cttattaaag
     7501 ccaatcaatg ctttgacacg ttcaatacca actatgatat tggtagtact tgcacttatt
     7561 tggtttgaaa aggatagtac cccttttata gttggattta ctatagtttt tcctatattg
     7621 tatgataacg ttttgggcgc tatattgaat atagacaaga acttattaga aatggctaat
     7681 gtgtataaaa taaggtttat agataaggtg ttaaaaattt atttaccagc cataaaattt
     7741 caaattatat ctatactagt atcaacattc tctttaagtt tgaaagttgc tatagcagga
     7801 gaggtatata gtcaacctac atatggtatt ggaactatga tacagactga aaaaattaac
     7861 tttaacacat ctggaatatt tgcatggata attattgttc ttttaatatc tgctatttta
     7921 caaatagctc aaaagttttt agcaaggaga gcttttttat ggaaaagata aagttgagta
     7981 ttgaaaatat aaataaaaga tatgatagtc gtattatatt tagagatttt aatattgatt
     8041 tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg ttgtggaaaa actacactct
     8101 tgaacataat aagtgggatt attaaaaatg acaccaacaa tctaaatatc aaagaaaatt
     8161 taaatagagt aggaaataag cttgaagcta gttacatatt tcaagatgat agacttatag
     8221 actggttaac agtagaagaa aatataaaga tagttgtaaa taaatactat aataaaactc
     8281 aattaaataa aatttgtgat gaatacttgg aattggttgg aattagtgat tataaaaaat
     8341 tctatcctca gatgcttagt ggaggtatta gacaaagagt aaatattgct agagctttta
     8401 tatatccatc aaaaaatatt ataatggatg aaccttttaa atctattgat gcaaagaata
     8461 cacagcttat aatggataat ttcagaaata tattaagaaa agaaaaaaga actgtgttat
     8521 ttgtaactca taatattgag gaagcattgt ttttagcaga taggatattt attttaggag
     8581 actctccaat tagaataaaa aaaatattaa aaaattctaa ggaattggaa aaaaatgaag
     8641 ttttgaagtt gatatagagt tataataata ctgtcgtttt aaaatatatt tggttaaaat
     8701 ctataagact atattaaaat agtacagaat attcaaaact gtgtataatg ttcgtatttt
     8761 ataaaaagga agtgaatgta tggttaagac taatgctatg agaatattag attcaaataa
     8821 gattgattat aaagtaatgt cttatgaagt gaaaagtgaa catgtggatg gtgttgaggt
     8881 ggcacatgat ataggaagag atgtaaatga agtgtataag acattagtaa ctcaaggagt
     8941 tagtaagaat atatatgtat atgtaatacc agtacatgaa aacttagact taaaaaaggc
     9001 tgcaaaagta gctaaagaga aaagtgtaga gatgattcat gtaaaagata taaataagct
     9061 tacaggttat attagaggcg gatgttctcc tataggtatg aaaaagcttt ataaaacttt
     9121 tgtaaatgaa agtgcaaaga atttagatac aattattgtg agtgcaggaa aaataggata
     9181 tcaaatagaa ctatctccat ttgatttaca aagacttata aaagtagaat ttgtagatgt
     9241 aataaaaaaa taagaattac atataaaata tagagcttat attcttaata ataggtgttg
     9301 tagtattaat aaaatgaata ataattttaa aataaatatt cagttactta tgtgtaattg
     9361 gtataaaaat attgagaaat tttaaaaaca atggtaaagt gtaagtataa tcacgaatat
     9421 tttttataaa gtaatggagg gatgttcgct tgagaaaaat aaaatctgaa caaatcgtcg
     9481 agcaggtaaa aaaactttgt atagaagcga gcctatattt aggagaagat gttttaagct
     9541 gtataaaaga aaaagcgaaa agtgaaaaga gtgaagttgg aaaaaatata ttgaatatat
     9601 tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc aatatgccaa gatactggta
     9661 tggctgtatt ttttgttgaa ataggacaag aagttttaat tgaaggagac acacttactg
     9721 atgctataaa tgagggagta agacaagggt atgaagaagg ctacttaaga aaatctgttg
     9781 taagtccaat taacagagta aatacaaaag ataatacacc tgctgttatt cattatgata
     9841 tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa gggatttgga agcgaaaata
     9901 tgagtaagat gaaaatgtta aagccatcag atggattgga aggaattaaa aaattcataa
     9961 tagatacagt ttcagaagct ggacctaacc catgccctcc tatggtaatt ggagttggta
    10021 taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa ggcacttttt agagaactag
    10081 gtgaatttaa taaggacgaa aatatagcaa aacttgaaag tgaactatta acagctatta
    10141 ataaacttgg tataggacct caaggcttag gtggtactac aacagctttg ggattaaaca
    10201 tagagacttt tcctacacac atagcaggtt tacctgttgt tgtaaatata aactgccatg
    10261 catcaagaca taagaaggta gtaatttagg aggaaaataa aatgataaaa ataactacac
    10321 ctgtaaatga aattgatata gcaaaattaa attgtggaga tacaattagt ttgagtggta
    10381 tactttatac agcaagagat gctgctcata aaagattgat agattgtata aataaaggtg
    10441 aagaattacc atttgacgta tatggtcaag gaatatatta tgtaggacct actcctacaa
    10501 agccaggaga agttataggt gctgcaggtc caacgacgag ttatagaatg gatgatttga
    10561 ctatacctct tttagaaaga ggtcttagat tgatgatagg caagggaaaa agaagtgatg
    10621 aggttataga aggtatgcaa aaatacggag cagtttatct tgctgcaata ggaggagctg
    10681 gagcatatat ttcaaattca ataaaatcat gtgaaattat tgcatatgaa gatttaggag
    10741 ctgaagctat aagaaagatt gtagtagaag accttaagct tacagtagca atagatagct
    10801 atggaaataa tatatatgag caaggtcgag caatttatga atgtaaataa tgtttagaaa
    10861 taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt taaggaggaa aattgatgtc
    10921 taaggattat gcaaaattgg cattagaaat gcatgaaatc aacaaaggga aagtaagtgt
    10981 agaaagcaag gtagaagtta aaactaagga tgatttatca acagcatata caccaggggt
    11041 tgctgaacct tgtttaaaaa tacatgaaaa tcaagatgat gtttatagat acacttctaa
    11101 agggaatcta gtagcagtag ttagtgatgg aagtgctgta cttggattag gtaacattgg
    11161 tgctgaagca tctatacctg ttatggaagg taaagctata ttatttaaac aatttgcaga
    11221 tgtagacgct tttccaatat gtttaaagac taatgatgta gatgagatag ttaaatctgt
    11281 agagttgatg gagcctgtat ttggaggtat aaatttggaa gacatatctt ctccaagatg
    11341 ttttgagata gaagagagat taaaaaaatc tttaagtata cctgtttttc atgatgacca
    11401 acatgggaca gcaataatag ttgcagcagc cataataaac tcaataaagt taattgaaaa
    11461 taaaaaaata gaagatttag aaatagttat aaacggagca ggagctgctg gtatagccat
    11521 agcaaagata ctacttaata tgaatgttaa aaatataatt ttatgtgata gaagtggagc
    11581 attggaagct agtatagaaa atctaaacta tgttcaaaaa gaaatgttaa aagtaacaaa
    11641 tataagaaat gaaaaaggac ctttaaaaga tattataaaa ggaaaagatg tatttattgg
    11701 tgtttcaggt ccaggagctg ttacaaagga aatggttgaa agtatgtctg agaagccaat
    11761 aatattggca atggcaaatc ctactccaga gataatgcca gaagaagcaa aattaggtgg
    11821 agcatttata atgggaacag gtcgttcaga cttccctaat caagtcaata atgtactggc
    11881 ttttcctgga atatttagag gtgcacttga tgttaaggct aaagagataa atgaagaaat
    11941 gaagatagct gctgcatatg caattgctaa tacaataagt gatgaagaaa tatgcccaga
    12001 gtatatatta ccagatgtat ttaataagaa tgtagtaaaa aatgttgcta aggcagttaa
    12061 agaagcagcg ataaaaacca aagtaaatag aatataaatt tttatatgtt catatatcct
    12121 tatcttatcg ggtatatggg catttttatt ataggtttat actcatatat ccttactttt
    12181 atatacatat ctttatgatt aaaggtgtta gaaaatgtag taaattttat actatcagac
    12241 tctatcttta ttactttagt agatacatgg tggtaaaggt ttatagaaaa aaatatagga
    12301 aggcagattt ttattgttgc gtatatatga gaatgatata atatgaatat tataaattaa
    12361 taaatttaaa tttatggagg tacaagagat gatggaatat aaagttaatg accaagaact
    12421 taatgcttca atgtttatcc cattcgtcaa caggatatgg ccaggagatt ataatatgga
    12481 taaaacacag actgcattat caaaaacttt gaatataact gcttatgatg ataaagtgct
    12541 tgtaggatgt ctgcgagttc tttcagatgg ttattatttt ggaacaatta cagaattgct
    12601 tgttcttcca gagtatcaga aacaaggcat tggtagtaag cttttccagc ttgctaaaga
    12661 gaacacccca actatgctat attttggtgc acagccagga gtagaagagt tctatgaaaa
    12721 aaatgggtgc caaagaagtc tgcaatccta cataataaaa aagaaagata gttattaaag
    12781 ttccagcttt caataatctt aaaagtacaa tattttagtg gaatcatttc attttttatt
    12841 actaatatga cataaacatg tcatattagc attgatatac ttttaatatg gttaagagcc
    12901 aaagaaaaat ataaggagtg atttaaaatg gaagaaacta aattttgcca atgctgtgca
    12961 atgccaatgg gacaaactga tgaattatat gggacaaata aagatggaag taaaagtact
    13021 gattattgta gttattgttt taaagatggt gagtttacag ctgatataag tatggaagag
    13081 atgatagaag tttgtatccc tcatatgctt caaagtaata aggatatgac tgaagatgaa
    13141 gcaagaacaa tgatgaacaa tttctttcca acattaaaac gctggaaata attttatgca
    13201 aaattagtta tatgcaaaac atgaaatatg gtgttgagaa taaggggtgg tgtgctttat
    13261 agcataccac ctgttttgtt acttggagtt cagttatttt cctaaatatt ttaaatatac
    13321 tattttagat aagttccttc ttcatcttga taaattaatc cttttttcat aagcactcca
    13381 agagctcgtt taaaattttt cttacttgaa ttaaataata tagaaatatc tttagggtca
    13441 gatttatcat taaatctcat atatccatca gaaccttcta aataacttaa aatcttattt
    13501 tctaaagtat caagttcatc ctttctattt cctctaggag aaagaccaag ttttccatct
    13561 tcatatattt ttatgacatg taaattttca agaacatcac cagtttttag ttcagtaaag
    13621 tactcattat gaagtattac accagcatat ttattgtcta cacatatcat tgcagaatta
    13681 ttagtttgga atccatatac aactccagtt acaatatctc ctacattgta agtatggtct
    13741 gttaataagt atgaatctat atctgtagta gcagcaattc ttttgctttt gtctaaatat
    13801 atataaaata aatatttttc gtctctaaat agtggataag tcttagcttt aaaaggaact
    13861 aatatgtctt taggaagtcc catatctata aaagtaccaa tgtctgtatt atcaactact
    13921 tttaaatgtg ctacatcccc aaccttagct aaaggcggta ttaaagttgc agcagttctg
    13981 tcatcagaat ctttaaatat gaaagcattt acttcatcac ctatgtttat ttcatttttt
    14041 ccgattaatc tattatgaag aagtatatcg tcttttgtgt tattagtttg tccatccaaa
    14101 aaatatccaa attcagtttt tctttttact gtaagtttat taaaatcacc tatgtttatc
    14161 aaattaaaat catcctctca ttttatattt ataatttagc cataatatac ttatgtagta
    14221 gtttgtcaca gtatctatat tagtattagt gaaatttaca ataaataata tagttattta
    14281 tgtatttttc aattctctag cttcttatat agtataaact ccaatttctt ttttgtcttt
    14341 aggttttatt aaaattttta caaaataagt taaaaagtat tgaaataaaa tttcttttaa
    14401 agtataattg gtatatacca aaattggtat ataccatatc gaaaagtggt gactatatga
    14461 aaatagtaaa taaatctagt aatataccat tgcatactca attaagttca ataataagag
    14521 agatgataga aactggtgag cttaaagagg gcgatgcaat aatgccagaa agagaacttt
    14581 gcaacataca aaatgtaagt cgtatgacag taaataaaac cattgttgga ttagttacag
    14641 aaggacttct ttatagggta caaggtaaag ggacattcgt tgcaaagcaa aaaaagaagt
    14701 atcagttttc aaatgtaaaa ggttttactg atgttatgaa agaaaaggga gtaaacataa
    14761 aaacggatat attatccttt gagatggaac taccagatga tttagttaaa agaaagcttg
    14821 gaataagtga taatactaca aatatatata agattgtcag acttagatat actgatggag
    14881 agccatttgg attagaaata gtatatctat cagaagaaat gtgtaagggt ctaacaaaag
    14941 ggatattgga taattcttca ttatataggg ttttaaatga aaaatatggc tataagatac
    15001 aaaaagctga acaggtaatg gagccagtaa tactttcaga tgaagaaagt aagcttcttg
    15061 aaacagatga aggtgcttta gctcttaaac ttcatagaaa ttcctataat agagaaggta
    15121 gccctataga atataccata tcaatattta gaacagataa atatcaatat gaaatagtat
    15181 tgagtgagta ggtgaataat atgaaatgtt taattaatgg aaagataata ttaaaaaatc
    15241 aaatcttaga aaataaagta ttagtatttg atgaaaaaat aattgatata gctgatagtg
    15301 taccaaaaga ttgtgaagtt atagatgctg atggaaaata tatatctcca gggcttattg
    15361 atattcatat acatggaaac atgggaaaag atacaatgga ttcgactgat gaatctattg
    15421 aaacaatttc aaaatcaatt atgagacatg gagtaacatc ttttctgcca actacaatga
    15481 ctatggataa agaacatgtt tatgatgcac ttgaagttat taaaaaagcc caaaatagaa
    15541 agcttgaagg ggcaaaggta ttaggagcac atttagaagg accttttata aatgagaatt
    15601 ataagggagc acaaaatgaa aaatttataa taaattcaaa atacgaattt ataaaagaat
    15661 ataaagatgt tataaaagtt ataacttatg ctccagaaaa agatattgat tttgatttta
    15721 ctagagaaat aaaaagatgt acagatatag ttttatctat tggtcatagt aatgcaaatt
    15781 atgaccaagc taaagaagct ataaatttag gagtaactaa tgtaactcac atgttcaatg
    15841 caatgactgg tttaaaccat agagacccag gcgttgttgg tgcagcactt acaactaatg
    15901 tgtacagtga gttaatagca gatacaatcc atataaataa agatttattt caatttatac
    15961 taaataataa agggaaagaa agactgattt taataacaga ttctatagaa gctggtggtc
    16021 ttgaagatgg caattattct cttggaggtc agaaagtcat agttaagggt aatgaagcta
    16081 gacttgaaaa tggagcatta gctggaagtg tgttgtcttt aaataaaatg gtatttaact
    16141 tcttagataa tacaaacctt aaagtgaatg aagcaattaa tttagcttct ttaaatccag
    16201 caacatcttt gggaataaat gacaaaaaag gaagcttaga aataggtaag gatgcagata
    16261 tagctgtatt tgatgagaat ttagattgta aaatgacact ttgcttgggg gaagtagtat
    16321 ataaaaatat atagtataca aaacaaacct taaagacagt aaaaacaata taaaaaataa
    16381 taaattaaac ttacattagt aaaagtagta gttaaaattt aggaggaata agatgagagt
    16441 attagtgtgt aaagattatg atggaatgag taaaaaagcc gctgaaatga tagcagctca
    16501 aatagtttta aaaccaaatt caatactggg actagctaca ggaagtacac cagtaggtat
    16561 gtatagagat ttagtaaaaa aatacaatga taatatagta gatttttcag atgtgatgag
    16621 ctttaattta gatgaatatt ataagttacc aatatctaat gaccaaagct atgattattt
    16681 tatgaaagag aatctattta atcatgtaaa cataaagcca gaaaatactc atctaccaaa
    16741 tggaatggct gatgatattg aaaaagagtg tatgaactat gaagcttcta tagatgcagc
    16801 tggtggtata gatgtacaag tacttggaat aggtcgtaat gctcatatag gttttaatga
    16861 acctgatact aaatttgcaa aaagaactca tgtagttgaa cttacagagt ctacaataga
    16921 agctaatgca agattcttta aatcaagaga agatgttcct aaaaaagctg taagtatggg
    16981 aataggttca atattgaagt ctaagaaaat attgctttta gcttcaggag aagaaaaagc
    17041 tgatgcagta tataatactg tttacggaga tataactcca gaagttcctg gttctatact
    17101 tcaacttcat aaagacacta tagttatagt agatgaagca gcagcaagta aactgaatcc
    17161 aaaagattat aaattagtat aagaatagaa tgtagtttaa gtaaaaaata gctatcattg
    17221 agtaatcaaa tttatttaat gatagctatt tttatttaaa ttttttactt ctttttattt
    17281 aaattagtta ttattgttta aattacttgc ttattacttt ttacgaaaac tcgttatcaa
    17341 gaagtatcca aataagatgt taaaaattat tatgtttagt gggattctat caagcataat
    17401 aaaattacct ataattggga tattaccaac tggaaacata aagaagaata caggcatcag
    17461 tgatagtaaa aaagatggaa aacaaagaag aagtaatctt ggaatatcaa tataccaact
    17521 accatccttt ttgtattctc taatcaataa ttcagttcct agtaataaac caataccacc
    17581 agaggaaatt aaattaataa aaatgtatct tttaaagtct tgcatataac cagtgtaaaa
    17641 ttgagtatag aaatcataat ttaatttaac ggctaaaaaa tacataagca atagaatgat
    17701 taaaaataaa agatatctaa gagctttttt cattttaaat tctcccttca gaaatacttt
    17761 atttagtttt attttgttgg aatctgattt tatcatttag tttaagaaat ccataatatt
    17821 agtgaagaat gaaataagta ttatatatga ataaatattt ttagaattaa ggtgaattgt
    17881 agtgcttaat ataactataa tattaattgt ttgaatattc aataaaaagt ttgaaaaata
    17941 tcaattttga tataaactga ataaatacag ttgaatatta aattattatt ttatcaaaaa
    18001 tatgcaaagt actacacctt caaatatcac ctaaagtaat taaaaggcaa ttaaggtgaa
    18061 gtagttcaag tatttctaaa ataagtttaa taaattctat aataatttat tttttgcctt
    18121 aaaattgcct taaaatccaa acaattttaa aactaatgta ctataaaaag ctggtataat
    18181 ttaaatatag taaaaaataa gatttatctt atctattggg atttaattat ttaatcaata
    18241 tatttatacc atttaattta aataaaaagt ctattaagga gtgagcttat gagtaagtta
    18301 atttatatag cagatgatga agataatata agaaatttgg taaagacatt cttaaaaaat
    18361 gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac ttttagaaca atttaatata
    18421 aaggagtgtg acttagtaat actagatata atgatgcctg gctcaagtgg ttttgaagta
    18481 tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca tgcttactgc aagagataca
    18541 gatatagatt atattacagg gataacctta ggtagtgatg attattttac aaaaccattt
    18601 agcccaatgt ctttggttat gagagtaaaa tctatattta gaaggataga gtttgaaaag
    18661 aaacaaaatt atgataaata tagtaactcg atagatatgg aattaaaatt tggtgatgta
    18721 ataataaata aaaagaataa aatcgttaca agtaaaaatg taaatataga ccttacacca
    18781 aatgaatata acctattgac ttatttgttt gaaaatatag atagagcagt ttccagagat
    18841 gaattattaa acaaaatatg gggatacgat atagaagttg agacaagggc tgctgatgat
    18901 acagtgaaga gattacgtaa aaaaatttta aatacaaata ttttaataga aactgtatgg
    18961 gggtttggat ttaggttgaa ggagaaaagt taagatgaag aatggaaatg acaagaatag
    19021 aattggaaag attaaatcca aatttaaatt aaagttctca aagaaaaata taagcataaa
    19081 aactaggatt ttgttgagta tactttgttt gataattata gtatttatga taatattagt
    19141 atcttttaat gtatttcttg atacatatat aaaaacaact gcgaatgaag aattgacaaa
    19201 gtctacagaa atagtagaac atatggatag taattttaga cctattaagc caccacaaga
    19261 tgatggaaag cttcctccca aagtattatc taattttatg agaaatgtac aagataaagt
    19321 taggatggca gaaacacaat cagatgctga tgctatggta gttgattcta agtataacct
    19381 gatatttcca acaagagagg atgatttctt aaaaaatgtt gatgagatgg agctgattag
    19441 ggagtgtatt caaaatgaaa aattagattt aaattcagat gcaaatacac ggatatctac
    19501 cagaaataag gattattata tttcaactgt aaaaattact agtatatatg gtgaacaaga
    19561 caagtatctt atacttttta tagacatatc aaaaactcta aatctagcac aaaagataaa
    19621 tattgtatta atatctgtaa tgtgttttgc tggaatatta gcaatattta cagctgtgat
    19681 tttatctgag aaaattgcta aaccgataaa agagttgtgt gagtttgcaa aaaaaatggg
    19741 tcaaggagat ttcaaaagaa cctactttga cttttcagat aaagaattag tagagctctc
    19801 aactgttatg aataagagtg ctgagtatct ggataagtat gataatgaac aaaaaatatt
    19861 ttttcaaaat gcatctcatg aattaaggac accacttatg tctattaaag gttatgcaga
    19921 agctataaaa tataatgtta tagatagtaa acatgcaagt gatataattc ttgaagaaag
    19981 tgataggtta agtgatatgg ttgaagattt attatacatt tctaaaatag ataatataac
    20041 taaagatttt gaacttgtag aatgtgattt aagagaagta ctttcaaatt gtggagctag
    20101 gcaaaatgta agagctataa ataaggggat aaaatttatt tatgaatttg atgaagaaat
    20161 ggttttattt gaatgtgatg aaaaaaacat ttccaaagca tttatgaatc ttatagaaaa
    20221 tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt aagtataatc aaaaaaatat
    20281 agttgtgata atagaagatg atggaattgg aatcaagaaa gaagatttac ctcatgtttt
    20341 tgaaagattc tataagggag tagggggaaa tcatggaatt ggattatcaa tagttaaatc
    20401 tattgtaaat aagcatggtg ggcgtatata cgtagaaaat ggcaaaaaag gagctaaatt
    20461 tactatatta tttaaattgt aaaaaaatta gagaaaatat agagatgttt gaagttgttt
    20521 tttatatata tctgttgtat actgttgaaa aaagaacaat attaagctga catatattac
    20581 gatagttgac atttaaagta aatgcttaaa tatagatacg aaaggaaaag taaaatctga
    20641 agtgtattta ggtttaagta agcttcttat tttataaaaa atatgtatga aagaacagct
    20701 gatttttaat attacaaatt tagtaacggt tgtatttgaa gcttttgtca tccatatgtt
    20761 tttatcagac tttttaggcc agaaagaagg gtatataaaa gttgtaagat atgctaaact
    20821 gggttttata atatgtttag gattctgtaa tttaataact ttaaacccta aaatcacaat
    20881 gcctttaatt ttcatcctta ttttttcaac atcattttta tataaaggaa atttaaagac
    20941 acgattgttt actactgttt tattaagtat attctttata ctgtcagaaa ttgtagttac
    21001 aagtattttt gttttatttg taaaagaagg ctttgaaata atgcttgaaa ataatagtat
    21061 aagagtattg gcgacaattt tatctaaaat tgtctttttg ttgacttgta aaattatttg
    21121 tctatttaaa aaagatgtac atttagatat gcccataaag tattggctcc ctttattttt
    21181 aatacctata tttagtttat ttctgtctgt ttctatcttt gatgtaagta aattttttag
    21241 tcttgaaagt ttaaaatttc taagcttaat atctagtgtt ggtattttat atattaattt
    21301 tattgtcttt tatttattta aatttattat agataaaact aaactatcta tgaaatatga
    21361 actacttgaa aaagaaataa ttcataagga agaattaaga cttagtaatg agtgttataa
    21421 aattattgtt gaacagactg attctgtcgt tttcgaatgg aatatcaaag aaaataaatc
    21481 atttgtatcg caggcttgga ctgaaaaatt tggatataac aatgcatgta agaatatttt
    21541 taaagaaatt aaagataaag acttagttca ttctgaagat aaagctatat ttgaaggttt
    21601 tcttgaaagt attaaaaaaa agaatatgca caatcaagca gtatatcgtt taaaaaaatc
    21661 taatggtgaa tatatttggt gtagaacatc aattacaagt atttataatg atgaaaatga
    21721 acttttaaga gttgtcggag taattgtaga tgtagatagt gatataaaaa aatatgaaga
    21781 gttaagaact agagcagaaa gtgactcatt gaccaatata tacaataaag gtacatttga
    21841 aaaattagtg gaagaaacta tagttatgaa tacaggtgat aaaaaagatg cattattcat
    21901 tattgatttg gatgatttta aagaaataaa tgataatttt ggtcatccat ttggtgattt
    21961 tgtactaaaa acatttgctg ataagataca aactagtttt ggtagtaaag acttagtcgg
    22021 aagaattggt ggagatgaat ttgttgtata tatgcaagat tatgttacag aagtaaattt
    22081 gcataaaaaa gctaaagaat taaatagagt actttcggat aattacactg atttgagttt
    22141 tagttttgat gcttctgtca gtataggtat tgcaaggtat cctcaagatg gaacttcatt
    22201 ttttgaattg tttaaaaatg cagatagagc actttatagc ataaaagcat caggtaaaaa
    22261 ttcatattgt ttatttgaag aagaactata tgtacagtga atttgaattt agattttatg
    22321 tgagagtaag atttatatat acttgtggct gtctgattta taaaatatgt tactttgtat
    22381 atttttatat gtttcagtaa ggttaattaa catgagcaaa ggtgtaagta aatttatatt
    22441 agtttaagtt tacttacact tttataaaaa tacaaataat agattcattt acattcttaa
    22501 ataattaaag ctctttacct tctaaacatg cataacatat cgttataatt ccagtaaata
    22561 agactaaaat gcccattcct aagattacaa tttcatcatc tattactcca aatgttttta
    22621 aagtaactta tttcgctttg tatcataata atttgtctcc ttttacattt taacttctgc
    22681 tgagatttga gttagatatt cattagaatc tcgcacactt atatcatata gaagatattc
    22741 ttcataaaca aaatccccaa tacgtaaatt attctgatta aaaaaattta ataacttttc
    22801 ataagtcttg taaatagttt cataactccc tttatgataa gcagttacat aaagtccctt
    22861 tggtttgatt gatacaggat atttcttgta ctctttatta acttttgtaa ataaatatga
    22921 atatgattca aagtctttat taattatatc ttcaccttta atgattgagc caatcgtacc
    22981 ataatcactc aattcatagt tgtccataaa gttaatacat tcatataaaa aattttcttc
    23041 ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt ttgaccaagt actcttcttc
    23101 atgttctttt aaagtaatag tattcgcatc tatattacaa gcatttttag tgaatgaaat
    23161 agtagattcc attagagctt gaatattatt tagtttttca atttcatttt taatatcaat
    23221 ggttttcttt tctaaaagct caattagtaa attaggtgtt cttttgtcta tataagcttt
    23281 aatttctttt aaaggcattt ttacttccct aagtgaagaa attataccaa aaatgctaag
    23341 ttgatggtat gagtagtatc tatatccatt ttctttcttt aattctggtg agaaaagacc
    23401 tatatcatca taatggaaaa gtgttttttt attgattcca caaatctttg caaattctcc
    23461 agttgtaaaa taattttttg aaacttgact cataattata attcctcctt gactatcctg
    23521 ttacagtata gtttatcata gtatttgtaa aggagtgtga ttatatgaaa aaattaattc
    23581 attttttaaa accatataga gttttaattg ttgtagtttt gatatttacg tttttacaaa
    23641 cacttggaac tttgtatata ccaacactta cagcaaatat agtaaacaat ggtgtagtaa
    23701 agggagatat agattatatt gtaaaaacag gtttaatgat gatgatagta gctggtataa
    23761 cagctttatc agctgtgtta gtttgtaaag tatcagcaaa tctttcatct ggattttgta
    23821 gagatattag agaagcagta tttataaaat cacaagattt atcaataaat gattttaata
    23881 atataggtac agcatcaatg attactagga gtactagtga tattacttta ataggtcagt
    23941 ctgtgtttat gtttattcaa ttagtattac cagcaccaat tataactgtt tctggtctat
    24001 tcttagcata ttctatagat aaagcaatga caattattat tgttgtagta atgtttttat
    24061 tcatgctttc agcattttta gttggtaaaa agcttataaa gttgtttaaa atgatgcaaa
    24121 taaaaatgga caatatgaat agggtgttac gtgaggtagt aactggagta agagttataa
    24181 gagcttttaa taggagtcgt tttgaaaaaa agagatttga tagaactgca atagattatt
    24241 ctgaaacagc aatttcaata aataaaatat ttgcagtact tatgcctatt gtaatgttaa
    24301 taatgaattt aggtatagta agtattattt ggtttggtgg gatgagagtc agtaatggaa
    24361 atatggaaat aggtcatata atggcacttg ttgaatattg tatcttaatt ttattctatt
    24421 taatcatggg agtaatggta tttatgtaca tacctagagc aggtgcatgt gcagatagag
    24481 taaatcagat attagatata gaacctgaaa ttgtagatgg aaatggtcat aaagatactg
    24541 tctcagagag aggacattta gtatttaaaa atgtaacctt ttcatatgca caatcagaag
    24601 aacctgtgtt aaataatata acatttgaag caaaatctgg agaagtaact gcaattatag
    24661 gaagcactgg ctcaggcaaa tctactattg caaatataat accacgtttt tttgaaattc
    24721 aatctggaga aatatctata aatggtcagg atattaagaa gattcctcaa aaagaactta
    24781 gagataagat tggatttgta ccacaaaaag cttttttatt tagtggaaca atagaagaaa
    24841 atataaggta tggaaaagaa gatgcaagta ttgaagaagt aaagcatgct gcaagtatag
    24901 ctcaagctga tgaatttatt tctgatatgg aagacaagta tgactcattt gtagcacaag
    24961 gtggaaataa tttgtcaggt ggtcaaaaac agagaatatc cattgcaaga gcgttagtta
    25021 gaaagcctga agtatatgtc tttgatgata gtttttctgc attagatttt aaaacagata
    25081 aaagacttag aaaagcgttg aaaaatgaaa tcaaagattc atcagctata attatagcac
    25141 aacgtataag tacaatcatg gatgctaatc agattatagt tttaaatgat ggaaaaattg
    25201 taggtattgg aaaacataaa gatttacttg aaaattgtga agtttataag caaattgcgg
    25261 attcacaact tagtaaagaa gaacttgcat aagatgaaga attaatctga tatttggagg
    25321 tgtatgatat gagtgaaaaa agaatagatg aacaagatat agaagtactt agttttgaag
    25381 atggcatgtc agcagaaaaa gctaaggatg caaaaaaaac tactaagaga ttattaaaat
    25441 atatggcaaa acaaaagttt aaaatattaa ttatttttat ttcagtattg ataagttcag
    25501 cgcttacagt tttagcacct atggttatgg gaaaagctat tgaccaacta tttaatggaa
    25561 taaaaactgc agtccaaaca ggtacaaaat tcagtgtgaa ttttagtact atggggggta
    25621 tagtatcaat acttctaggg ttgtacttaa ttagtgcagt atttatatat attcaacagt
    25681 atattatgtc aggagttgca caaaatctag ttcttagcat gagaaaagat ttaagtgata
    25741 aactcaataa acttcctctt aagtactatg attctcataa aaagggggaa acacttagta
    25801 tagtaacaaa tgatttagaa aaagtagcag atagtttaca agaaggatta atgcaactta
    25861 ttacagcagt agttacagta gttggttcta ttgtaatgat gataagtata agtgtaccac
    25921 ttacaatagt atcagctata acattacttg tttcattggg tattactgtt gtaattgcaa
    25981 gaaaatcaca agaaagattc tctgaaaatc aaaaggcact aggagagtta aatagcaata
    26041 tagaagagat atttactggt caaatagtgg ttaaagcatt tagtaaagaa aaggatacta
    26101 taagaaactt taaaatatta aatcaaaatc tttataatgc aagtagaaaa gcacagtttt
    26161 cgtcatatgc aatatctcca attattagat ttataaacct aattggctat gttattatag
    26221 cagttgtagg tgggatattt gcatcaactg gagcaatgac tcttggtagt atacaagcgt
    26281 ttattcaata tgtaaatcaa gcatcagagc ctactacaga aatatcttat attgtaaata
    26341 tgcttcaagc agcaatagca tctgcagaga gagtttttac agttatggac gaggtggaag
    26401 agataaagga tgaagaatct tcaaaggtta ttagcatgcc taaaggtaaa gtacaatttg
    26461 agcatgttaa atttggatat agtgatgatt ttattcttat gaaagatatt aatatcaatc
    26521 taaatgcagg agataagatt gcaattgttg gacctacagg agctggaaag acaactcttg
    26581 taaatttact tatgagattt tatgagatac aaggtggaag aattactatt gatggtgtta
    26641 atataaaaga tttaaagcgt ggagaactta gaactatgtt tggtatggtt cttcaagata
    26701 catggctgtt taatggaagt ataaaagaaa atattgcata tagtaagtca gatgccacaa
    26761 tggatgaaat tgtaagagca gcaaaatcag caagagtaga ccattttata aggactttac
    26821 cacaagggta tgacactata ttgactgagg atgcttctaa tctatctcaa ggtcaaaagc
    26881 aacttctaac aattgccagg gcaatacttt cagacccatc agtattgatt cttgatgaag
    26941 caacatctag tgtagatact agaacagagg tagaaattca aaaggcaatg aataacttga
    27001 tgaagggaag aactagtttt gttattgcac atagattatc aactattcga gatgctgatt
    27061 taatattagt aatgaaagaa ggaactatta ttgaacaagg aagccataag gaacttattg
    27121 aaaagaaagg attctatgaa gaactttata acagtcaatt tacaagtgaa tatgatgaag
    27181 atgttgtatg attatcaagt tttaggcaac taaatgattg ataaatatta agttaaatat
    27241 agtaacatgt aaggttatat ggaataagag tatttctcta tttcatataa ccttttttat
    27301 ttatcaaaat aagataaaat ttcctttcta aattagattg aagaatgtat ttatcaatat
    27361 aaaagtaaaa taatcattat tattagtgaa gtaaatattg tttgccacat atatgccata
    27421 aagatgcact tattttacgg agatttaaca aagtagtgta aagtatactc taaatataaa
    27481 ataaatcaaa tagagttaaa taaattgaga ggtgatataa atagtgaata aaaaattgaa
    27541 ctatagattt gaaatgaagc acaaaataac tgaagcagat gtgttagctt taaaatcaag
    27601 actctatcca ataatgaaga aagatgaaaa tgcatctaaa gatggaaaat atcttattag
    27661 gagtttatat tttgatactc ctgaagataa agcacttttg gataaattaa atggcgttgc
    27721 aataagagaa aagtttagga taagatttta taataatgac tgctcttata taaggttaga
    27781 gaaaaaaatc aaacattaca acatgacatc aaagctatca actaatttaa ccaaaaaaga
    27841 agtaataaat atactaaata atgatattga atttctaaaa gagtcgacaa attgtttatt
    27901 aagagaattt tatttgaaat taaaatgtga aagattagag gcaaaaagca tagttgatta
    27961 caatagagaa gcttttattt atccagttgg aaatgtaaga gttacaatcg atagtgatat
    28021 taaaacttct gtaaattcgg tagatttatt taataaagat ttgccaactg tttcggtaat
    28081 tgatgataat atgactgtat tggaggttaa atatgatgaa tttataccag attttataaa
    28141 ggatttaatt caaattaata agactacctc tacagcagtt tcaaagtatg cttcaagtag
    28201 attatatatt taagaataaa ggctatgatt tatataaaga tatacataaa taaggttaaa
    28261 aaacaaataa gttaaaaata aataaaataa aaaataaata agttaaaaat aggaaggatg
    28321 attttaatta tgacatttaa tgatattttt aaatcaagtt ttatagaaaa cgtatcaggt
    28381 ttttcatttg tggacagtgc tttagcacta ggttcagctt ttttagtagg tttatttata
    28441 tatatggtct ataaaaagac ttatatggga attatgtatt ctcgaccatt caatgtgtcg
    28501 cttgtagcac ttacaatgct tacaactttt gtaatattgg cagttacttc aaatgtagtt
    28561 ttatccttag gtatggttgg tgctttatct atagtaagat ttagaacagc aattaaagac
    28621 ccaatggatt tagtatttct attttggtca ttgggttcag gtatagtttt aggagcaggt
    28681 ttgattccct tagctgtcat gggttcaata attatgggat tgattctaat attcttttca
    28741 aataaaacaa tatctgaaac tccatatata ttgatggtaa attgtaataa tgaagattca
    28801 gaagatatag caactgataa aattaaaaaa gtatttacta aatatcaaat aaaatcaaaa
    28861 agtgttacac cagaaaaagg aatagaatta gtgtttgaag taagaatgaa agatggagaa
    28921 acaagtctta taaatgactt gagtcaagta gatggagtta ctaatgcagt tcttgtaagt
    28981 tataatggtg actatgtagc atagagagga gtaatctttt atgaaagata aaaaatttac
    29041 ccttcttatc tcgattatga ttatattttt atgtgctgta gttggagttt atagtacatc
    29101 tagcaacaaa agtgttgatt tatatagtga tgtatatatt gaaaaatatt ttaacagaga
    29161 caaggttatg gaagttaata tagagataga tgaaagtgac ttgaaggata tgaatgaaaa
    29221 tgctataaaa gaagaattta aggttgcaaa agtaactgta gatggagata catatggaaa
    29281 cgtaggtata agaactaaag gaaattcaag tcttatatct gtagcaaata gtgatagtga
    29341 tagatacagc tataagatta attttgataa gtataatact agtcaaagta tggaagggct
    29401 tactcaatta aatcttaata actgttactc tgacccatct tatatgagag agtttttaac
    29461 atatagtatt tgcgaggaaa tgggattagc gactccagaa tttgcatatg ctaaagtctc
    29521 tataaatggc gaatatcatg gtttgtattt ggcagtagaa ggattaaaag agtcttatct
    29581 tgaaaataat tttggtaatg taactggaga cttatataag tcagatgaag gaagctcgtt
    29641 gcaatataaa ggagatgacc cagaaagtta ctcaaactta atcgttgaaa gtgataaaaa
    29701 gacagctgat tggtctaaaa tcacaaaact attaaaatct ttggatacag gtgaagatat
    29761 tgaaaaatat cttgatgtag attctgtcct taaaaatata gcaataaata cagctttatt
    29821 aaaccttgat agctatcaag ggagttttgc ccataactat tatttatatg agcaagatgg
    29881 agtattttct atgttaccat gggattttaa tatgtcattt ggtggattta gtggttttgg
    29941 tggaggtagt caatctatag caattgatga acctacgaca ggtaatttag aagacagacc
    30001 tctcatatcc tcgttattaa aaaatgagac atacaaaaca aaataccata aatatctgga
    30061 agagatagta acaaaatacc tagattcaga ctatttagag aatatgacaa caaaattgca
    30121 tgacatgata gcatcatatg taaaagaaga cccaacagca ttttatactt atgaagaatt
    30181 tgaaaaaaat ataacatctt caattgaaga ttctagtgat aataagggat ttggtaataa
    30241 agggtttgac aacaataact ctaataacag tgattctaat aataattcta atagtgaaaa
    30301 taagcgctct ggaaatcaaa gtgatgaaaa agaagttaat gctgaattaa catcaagcgt
    30361 agtcaaagct aatacagata atgaaactaa aaataaaact acaaatgata gtgaaagtaa
    30421 gaataataca gataaagata aaagtggaaa tgataataat caaaagctag aaggtcctat
    30481 gggtaaagga ggtaagtcaa taccaggggt tttggaagtt gcagaagata tgagtaaaac
    30541 tataaaatct caattaagtg gagaaacttc ttcgacaaag caaaactctg gtgatgaaag
    30601 ttcaagtgga attaaaggta gtgaaaagtt tgatgaggat atgagtggta tgccagaacc
    30661 acctgaggga atggatggta aaatgccacc aggaatgggt aatatggata agggagatat
    30721 gaatggtaaa aatggcaata tgaatatgga tagaaatcaa gataatccaa gagaagctgg
    30781 aggttttggc aatagaggag gaggctctgt gagtaaaaca acaacatact tcaaattaat
    30841 tttaggtgga gcttcaatga taataatgtc gattatgtta gttggtgtat caagggtaaa
    30901 gagaagaaga tttataaagt caaaataaga ttaaaattga atataaataa ttttaatact
    30961 gttaagtcta aatatttact tagcagtatt tttttagaaa ataatgtatt tggttaataa
    31021 tataaggtaa taaattgata aaaattaaat acagcatatt aataatacat aataaagtaa
    31081 taaaaaaact gtttaagtat aataacttat atttagacag tttttttcat ttataaataa
    31141 tataaatgaa attgaaaaca tttgaaggat agaaattggt tggataaatg tataaattac
    31201 ttcaccaaaa aataatgaca aaaattgtaa ctaagttgta ataaaatggt agttatttag
    31261 cataaaataa tgtatcatag aagtatatta taaaattaat aaaataagat ttttgtaaga
    31321 aaactgctgg cttttgatat ttagagtgag tagttaaatt atgtttagga ggggtagaat
    31381 ttgttgcaaa aagtattatg gaaattttta aagctagtag tcggtttatt tatatgttca
    31441 gttggtatag tattgacgat aaattgtaac cttggattat ctccctggga tgtatttcat
    31501 caaggacttt caaatcatat aggaataact ataggaactg cttctataat tgtaggttct
    31561 atagtagtta ttgctgatgt cgttcttgga gaaaatgtag gatggggaac tgtttttaac
    31621 atgctactaa ttggattttt tatggattta cttttatata gtaatttaat tccagaggct
    31681 gatagtttat ttgtaggaat aattatgttg atattaggat tggttctttt gtctgttgga
    31741 atggtatttt atatgggaag tggtttagga agtggaccta gagatggttt gatggttgca
    31801 attcaaaaga aaacaggaaa atcacttaaa ttaataagag gtactataga agttggagca
    31861 ttaatagtag gatttttatt aggtggaaaa gttggtatag gaactataat aagtgcattt
    31921 ggattggggt atttcacaca aatggtattt ggcttattta aattagattg tagtaagatt
    31981 aaacatagat ttatagtaga tgatataaaa tttatcaaag tgtatatgag tggtgagaaa
    32041 aaggctgctg ttcaaaatat agttaagtaa tttaagaata aattcaacga aatgttaagt
    32101 attttaaatt aacattttgt ttagatagat aaattgaata ttaaaagaat attaaaatat
    32161 gaggattgct agaacactat aaaatataga tttctagcaa ttttttattt gttcaatatt
    32221 tttttagtac tagaagtctt attctctatt taatgaatga attaatttat aattagagat
    32281 actaaacttt atagatatta atataaacac ctttaaactg tgtattttcc aaagccttta
    32341 aaatgcttgt atttattcta aataaaaaaa taaaaaaatg tcaaaaaaac tcttgtatta
    32401 tatccaaatt ttatatataa ttacctatgt tgtttagaaa ttttaaactc aatgtttagt
    32461 aaacaaaatg tttagtaaaa taaaacagaa actaggaggt gcaacatgga tataggtgaa
    32521 aagataaaaa gattaagaac agaaaaacaa ctgacccagg aagagttagc taatagatgt
    32581 gaattgtcaa agggatttat ttctcaatta gaaaataact taacatcacc atccatagct
    32641 acccttatag atatacttga aatactaggt acaaatttaa gggaattttt taatgaaata
    32701 gatgatgaaa gaatcagttt tacaaaagaa gatatgtttg aaactgaaga tgaagattta
    32761 aaatataaat taaagtggtt aattccaaat tcacaaaaaa atgaaatgga gccaataata
    32821 ataacattgt atccaggagg acagtataaa gaagaaaagc cacatgaagg tgaagaattt
    32881 ggatatgtat tagcaggttc aatatatgtc catataggcg aaaagaaaaa taaagtaaaa
    32941 aaaggagaga gtttttactt tagaccgaaa gcaaatcact atatatcaaa cgagggcaaa
    33001 actacagcaa aagtgatttg ggtaagtacg ccaccatcat tttagaatag aggtgtttaa
    33061 taattggtag aaaatataat agaattaagg gatatatcta aacattatga agaacttact
    33121 gttttagata atttcaattt agatataaag aaaaatgagt ttctaacatt acttggacca
    33181 agtggatgtg gaaaaactac aacattaaaa ataatagcag gatttgagta tgctgatgat
    33241 ggtaaagttt tatttgaagg taaggagata aataatttgc caccatatga aagacaagta
    33301 aacacagtat ttcaaaaata tgctttattt ccacacatgg atgtttatga aaatgtagct
    33361 tttggattaa aaattaaaaa gactccaaaa gatgtaatag atgctaaagt aaaagaggtt
    33421 ttgaaacttg ttgcactaga aggttttgaa agacgacaag ttgaatcatt gagtggagga
    33481 caacaacaaa gaattgcaat agcaagagct ctagttaatg aaccaaaggt attattacta
    33541 gatgaaccac ttggagcact tgatttaaag ttaagacaag agatgcaaat agaattaaag
    33601 agaatccaaa aaaaattagg aataacattt gtttttgtaa ctcatgacca agaagaggct
    33661 cttactatgt cagatactat tgttgtaatg tataagggca aaattcaaca aatgggaaca
    33721 ccacaagata tatacaatga acctaaaaat tcatttgtag ctaagtttat aggtgaaagt
    33781 aacatatttg atggaattat gatagaagat tataaagtta atttctgtaa tagagatttt
    33841 gagtgtgttg ataaaggatt cgaaaaaaac gaaaatatag aagtagtaat aagaccagaa
    33901 gatataaaaa tggtaaagcc agaagaagga atgttaaagg ggaaagttac atctacagtg
    33961 tttaaaggag tgcattacga gatagaattg aatgaaaatg gaagattgtg gatacttcat
    34021 aatactaaaa atgctgaggt tgggactgaa ctaggcatgg atatatatcc agaagatatc
    34081 catataatga gaaaagagaa taactaagat gaaaagaaaa tcttttttag catatccata
    34141 tgtagtttgg agtgcgatat ttgtaataat tcctttaata ttagtagtat tttttagttt
    34201 tacaaaagaa tcaggtggag gttatgcctt tacacttgag aattataaag aagtaattga
    34261 ccctatatat atgaaagttt ttgggcgttc tattctttta gcaggaggag ctacattaat
    34321 atgccttata gttggttatc cagtggcata tataatttca aaggctcgtg taagtagaag
    34381 aggttcatta atattattgt ttattcttcc tatgtggatg aactttcttt taagaacata
    34441 tgcatgggtt gctatattag gaaaaaatgg acttttaaat acatttttag gttggtttgg
    34501 aatacaacca ttagcaattt tgtatactaa ttttgccata cttttaggta tggtgtataa
    34561 ctttttacca tttatggttc ttccaatata tacagcacta tcaaagatgg ataatgattt
    34621 gataaacgct gctcatgact tgggtgcaaa taatatgaca gtttttagaa aaattatatt
    34681 tcctttgagt ttgccaggtg taatgtctgg aataactatg gtatttatgc cagcagtcac
    34741 tacttttgca atatcaagac tgttaggtgg aggtaaaata atgctggttg gagatttgat
    34801 tgaacaacag tttacagttg ttggagattg gaactttggt tcagctatat cgatatttat
    34861 gatgatagta atattaatat ctatgtctat aatgtcaaaa tttggagatg aatctgacaa
    34921 agaaggcggt gggttactat tttaaaatta aagaaaataa cttctaatat atatttagca
    34981 ttagtattct tatttttgta tgcacccata tttgctttgg tactattttc tttcaatgat
    35041 tcaaaatcaa tggcaagatg gaatggattt acatggaagt ggtatggtca attacttcaa
    35101 aatgagagta taatgagtgc tttatattat actattgtaa tagctatatt agcatctgta
    35161 atatctacaa tagtaggaac tataagtgct ataggaatac ataagatgag aggtaagagt
    35221 aagaaattaa tactaaatgt aaactatctt cctattttaa atacagagat agttacagca
    35281 gtagctctta tgagtctgtt tgtatttgta aaaatggaat ttggatttac aacaatgcta
    35341 ttggcacaca taatgttttg tttgccatac gttatacttt cagttttgcc aaaaatgaag
    35401 caattacctg ataatataga agatgcagct atggacttgg gagcaacacc tatatacgct
    35461 cttagaaaag taattttacc acaaataaaa ccaggtatag tttctggatt tttaattgca
    35521 tttactatgt caatagatga ctttataata agtttcttta atgctggaaa tggtgtaagc
    35581 aatctttcta tagaaatcta tggaatggct agaagaggta taaaacctga aataaatgca
    35641 ttatcaacaa taatgtttgc agtggttcta ggtttacttt tactagctaa taaaaaagaa
    35701 tctatagtaa gggggattaa ataatgatta agtttaaaaa agcaatatcc cttatagctg
    35761 taggtatgat gatagttact ttatttacag gatgtaataa agcaactgat tctacaaaag
    35821 ttttaaatgt atataatgtt ggagattata tagatgagtc tcttatagat aagtttgaaa
    35881 aagaaacagg aatagatgtt caatattcaa cttatgatac taatgaaatg atgtatcaaa
    35941 aagtaaagag tggtagtaca cactatgatt tagtatttcc ttcagactat atggtagaga
    36001 aaatgaaaaa tgaaggtctt ttggaaaaat tagattttaa gaatatacct aatatgaaat
    36061 atttagataa gagtttttta aatcctgtat acgacaaaac taatgaatat agtgttgcat
    36121 atatgtgggg aactcttgga atattgtaca ataagaaaga agttaaagac cctatggata
    36181 gctggaatat actttggaat ccaaagtata agggaaatat aatgatgttt gactctgtta
    36241 gagatactat agggataact cttaaaaaat taggatatag catgaatagt gtaaatccaa
    36301 aagaaataaa tgaagctaaa aatcttttaa tgaaacaaaa ggatttagta ttggcttatg
    36361 taaatgatga gggtaaggat agattacttg gtggagaagt ggcaatgggt atgctgtatt
    36421 ctggagatgc agttacacta atggagcaaa atcctgacct tgattatgct atacctaaag
    36481 agggaactaa taaatgggta gatgctatgt gtgtacctac aacagcacaa aataagaaag
    36541 aagctgagct gtttataaac tttttattag acccagagaa tgcaaaagta aatgctgagt
    36601 acattgggta ttcaacacct aatacaggag cactaaaatt attagaccca gaaataactc
    36661 agaatcctgt tgcatatcca tctaaagaag tacttgataa atgtgaaact tttgaagata
    36721 ttggtgaaaa tataaaatta tatgacaagg cttggataga gttaaaatca aaatagggtc
    36781 ttgttggttt aagtaaattt acttaataaa tacttaataa aactgttctt ttttagaaaa
    36841 tttattttct aattattgaa cagttttatt ttttgtttaa gttatattca tatgcaattt
    36901 aaatagttta ttaataaatg aaaacttatt tgtataatga ctttaataat aattagaaaa
    36961 actgggcaac ttaatagaat aattggaggt gtaaatttat aaaaaatgaa taggataaat
    37021 aaaaaaatag atttattaat attggctttg ctcgttttta tatttttaat tgttggaata
    37081 gtaatactat cgattaggac agaaaataaa ttgaatgtgt tcataatgtt agcaataatt
    37141 ttttttataa taatgttaac ttatttatca aattcagtag tggggttgat aacaagttca
    37201 ataattatat ttatgtatac atcctatatt ttatataaca atattactca caatatggat
    37261 gttgaattta tatcatatat gtggataata gcaacaccag tatcttcaat tataatgggt
    37321 aatctgaaca aaagcataaa tgaattgcaa aatacaaata aaaaactatc tgaacagtat
    37381 aaggaattag tgactattga tagtgaaact ggacttagaa atttaaagat tttttataat
    37441 gatgtaaata tggagataag taaatctatt agacataata ctgatttttc tcttatgata
    37501 gtaaaattac catactacgg aaacctacag actatatttg gtgaaaacaa aactaataaa
    37561 atagtaaaat atattggttc aaatattata gaatgtacaa gaaatgaaga tataatttat
    37621 agtttacaaa aagatatgat agggatacta atgccaaata catctttgga aggttcaaaa
    37681 gttgttaaag acagaataaa gaaaaggata aaagaattaa acttagattt aaacaatagg
    37741 ggaaaatatg taaatataga tgtcaaaatt gcatttttac aatataagag tagttttgga
    37801 gatagtataa attttaagaa tatagttgaa gaggaacttc aatatgatgt ttaaaatatt
    37861 taataaaagt ctattaaaat tagtagttgt attatcaata gtactgacaa tatcaaattt
    37921 tagcttatct tcatacgcat cagaaattac ttccagcaca gataaggtat taatcatata
    37981 tgattctaag aaagaaacag cttataatag agatatttta aatattatga ggactctatt
    38041 gggaagattt agttcagata tagagctatt aaaattgagt aactatgatg gtgagataaa
    38101 taaaaattat tacagtcata tatttatttt aggaatcaat gaaaactcat ataataatga
    38161 taaaaacact aaaaacttga ttagttcctt aaattcatat aaaggaacta tatgttggtt
    38221 aggatatggt atagaaaatc ttttggaaca taaaaaatac aatttagact atgtaggaaa
    38281 aacaaacaat atagttagtg taaattatag aggtaaatca tataacttgg atgaacatta
    38341 tgtttttaac attgtagaat caaaagatac aagtaataaa gtaataggaa gtattaatga
    38401 taccttaaat aagtatccat atataataaa tgataaaaat cttttttatg tatcaaaatt
    38461 agatttagat ggagttttat tttatatatt ttgcgattca cttaatgata tttttaacat
    38521 taagacgttt gataaaggac gtatatttgt aagaatagaa gatgtacatg cttttagaga
    38581 accaaaaaat cttgtagaga tagcggatta tttaagtagt aagaatatac catttacgat
    38641 tgcattaata ccagcatatg taaatcctaa aaatcataaa gtaataactt tatcagaaag
    38701 ccctgaaatt gtcaaagcaa taaaatatat gcaagataaa ggtggaactg tgatattgca
    38761 tggatatact catcaataca agaaagaaga agtttctgga gaagggtatg aattttggga
    38821 tggtaaaaaa gatgaaccct taaaagaaaa tatgaagata tttgtcaaag atagagtttt
    38881 gaatggtctt agagtatgta tagagaatgg aatttatcct cttgcatttg aagcgccaca
    38941 ttatgcaatg gaatcagagg gatataaaga attaaaaaaa tatttttcta cttatatggg
    39001 gcaacatcaa aataatgata aaaaatttag cacaaataca tatccttata ttattaggga
    39061 tacagaggag tttaatatat ttatacccga aaatttagga tatatagacc cagaagataa
    39121 gtttactttt caaaatataa aagaaaattt agataaatta tctattgtaa gaggttttag
    39181 tggagggttc ttctttcatt catatttaaa catagagtat ctaaaaaaca caatagaata
    39241 tttagaaaaa cagaatatag aatttatgaa tttaagagat tttaataatt gggtaaaagt
    39301 tgatgaaata caaataagaa ataatggaga tgaaattata gtcaactatg ataaggattt
    39361 agatgaaata actaaaagtg atactagatt taaatccata tccaatatta gtaaaatttt
    39421 aatattcata gtatctataa gtgttttaat atttgtgatt atatttatat attttaagag
    39481 aatagataaa aagaaatttt taaaataaat gattttatat tagaatcaat attttaaaat
    39541 attttaggag aaatttatga gagatttaaa tgtatcagag tatttgttca tatttagtct
    39601 gtttagtata tggtctttat tgcttataaa tattatttta gctatgggtg gatatatatt
    39661 ttattttaaa aactttgata aagaaataaa agaaattgat gaatatccta tgatatcaat
    39721 acttgttcct gctcataatg aagctaaagt gataggtaga actgtggagt ctttgctatt
    39781 actcaattat cctaagagta agatggaact tattgttata aatgacaatt cttctgataa
    39841 tagtaaagaa attttagaaa atataaagga taggtacaac aattataatt tcactataat
    39901 aaatacagat agtttaacag gtggaaaagg aaagtctaat gcattaaata ttggatatac
    39961 gatttcaaaa ggagatttta tagctgttta tgatgcagat aacacaccag ataaaaatgc
    40021 acttagatat ttagtacaaa ctatagtaat gaatgatgaa ttaggtgctg ttattgggaa
    40081 atttaggact cgaaataaaa ataaaaacct attaaccaaa tttataaaca tagaaacatt
    40141 aagctttcaa tggatgtcac aagctggtag atggcaactt tttaatctat gtacaatacc
    40201 aggaactaac tttatattga ggagaagtat catagaagaa attggaggat gggatagtaa
    40261 agcaattgca gaagatacag aaataagttt taggatatat aaattgggat acaagataaa
    40321 gcttgtacca caatctataa catgggaaca agagccagaa actgttaagg tgtggataaa
    40381 gcaacgtact agatgggcaa aagggaatat atatgtatta atgaaatata taaaaaatat
    40441 atttaaacag ggaagaaata agattgtgtt tgatatagct tacttttttt ctgtatattt
    40501 tctattttta acatctgtaa ttatatccga tattttattt gttttaagta tatctaaact
    40561 agtagaaatt tctattccaa ttaacttttt tctaatatgg atactatctt atcttttatt
    40621 tattatagaa gtgagtataa gccttactat agaaaaaggt gaagcaacaa ttgaaaatat
    40681 attcatagtt gcaataatgt attttacata ctctcaacta tggctgtttg tagctataaa
    40741 aggaatgata gagtatctaa aagatataat ttttaaaaga gaggttaaat ggtataaaac
    40801 agaaagattt tgattcatgt acaggaggaa ttaacatgaa aaaatttatt atatcaatta
    40861 tatctttagt attgtttttt tcaaatatat cacttatata caaagtaaat gctgatgaga
    40921 ctaaagttaa aaattataag tttgaaagag atataactat tgatggtgtt ataggaagta
    40981 atagtacttt ttttgaagta aataaaaatt gggatattga agaagtttta ttgcatctta
    41041 attttagtaa gagccaaata ttaaatggag atgtttcaag tttaactgta ttgataaata
    41101 atgtaccaat aaaatctatt aaattaaatg ctaagacaaa ttataaaaat acattagaag
    41161 tattagttcc aaaagattat ataattcaag gatataatga aataaaaata aaaacataca
    41221 aaacaatatc tgataaaatt tgccaagatg attccaatac tgggaactgg atggttattc
    41281 acaaagaatc atatatatca attagatata agcagaaaaa ggtggaaaac tcaataaatg
    41341 aatatccgta tccatatgca gaaattgaaa ataatcataa attagataca actatagtag
    41401 taccagataa tatgactaga ggagagacta cagcagtgtt taatttggca tcagcatttg
    41461 gaaaaataac aaaaaatgat gatttaaagt tagatgttaa attatattca gaaatgaaaa
    41521 attggtcaga tgataatatt atatatattg gtaaacctga aaatacagca gaagaaattt
    41581 tggatatttt aagtataaag gaacaaaccc tattatcttc aaactgtata atcaagcaag
    41641 tagattcacc atacaataag aataagaaga tgatggtggt aattggtagt aatgaagatg
    41701 atttaataaa ggcatcaaat ttattaatag aaaatagact ttctaaccaa gttttatcat
    41761 catcagtact tgtaaacaaa gagactaata taaaaattaa tagggaacaa aaattaaatt
    41821 taggtcattt aacattaaaa gacttaggat attctgactt tttgttggaa ggagcattta
    41881 atcaacaagc tttgtttgat gtcaaaatac caacgggtaa ggtattagat gatggttcaa
    41941 agattatctt aaatttaaga tattctgaca atttagattt tgaaaaatct ttagttacag
    42001 tgtctataaa tgatgttatt gtgggaagta aaaaattgga taggtcacat tctaataatg
    42061 ataagttaga attaaaaata cctaaagata tagataataa aaactattat caagttaaat
    42121 tgacatttaa tttgagtata aaaaattcga attgtgttac tagagagagt aataatcctt
    42181 gggcatatgt atcaaataat tcttatttgg cgttatctac gaaagaaaat gaaaccttat
    42241 cttttgaaaa ttatccgtat ccatttgtta gagatgatga atttaatgat ttaactgtga
    42301 taatgccaga ttattcaggg tcacaagcta tgacctggat gtttagatta ggtgttactt
    42361 taggtgcgaa tataaattca cataatggaa atataaatgt aattagagga aaagaattta
    42421 gtgacaagta taaggatact aatattgtag tttttggagt gccacataat aattctgtta
    42481 taaagatgtt aaataataat ttaaatataa agttcgataa aaattactcc aattttatat
    42541 caaatgataa gataagcttt attgatgatt atggaaaaaa tatttctacc attcaattaa
    42601 taaaatcacc atataataat caaaaaaata tcatggtaat tagttcaatg aatgaaaaaa
    42661 acttatattt aggaatggat tatttgttga ataaaagtaa agttaatgat ttaaaaggag
    42721 atacattgat aattgatgaa tacggagagg tagaagattt agcttataat ttaaaatcta
    42781 agaaagaagt aaaagattca agttggaata tgagtataaa taagacaact aaagtatttt
    42841 taatgatttc ttttattact ataattgtag tcatgatatt atcaatgcta tatataaaaa
//
LOCUS       DAENCV010000023        42871 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAENCV010000023 DAENCV010000000
VERSION     DAENCV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.023"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(526..702)
                     /locus_tag="KQD82_001167"
     CDS             complement(526..702)
                     /locus_tag="KQD82_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379645.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1277..2299
                     /locus_tag="KQD82_001168"
     CDS             1277..2299
                     /locus_tag="KQD82_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379646.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGTMSLCFLVIK
                     SDYISR"
     gene            2313..3200
                     /locus_tag="KQD82_001169"
     CDS             2313..3200
                     /locus_tag="KQD82_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379647.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDILSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3206..3979
                     /locus_tag="KQD82_001170"
     CDS             3206..3979
                     /locus_tag="KQD82_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379648.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            4091..4816
                     /locus_tag="KQD82_001171"
     CDS             4091..4816
                     /locus_tag="KQD82_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379649.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4820..6223
                     /locus_tag="KQD82_001172"
     CDS             4820..6223
                     /locus_tag="KQD82_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379650.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6234..7058
                     /locus_tag="KQD82_001173"
     CDS             6234..7058
                     /locus_tag="KQD82_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG4379651.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYALMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7383..8003)
                     /locus_tag="KQD82_001174"
     CDS             complement(7383..8003)
                     /locus_tag="KQD82_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4379652.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8268..8600
                     /locus_tag="KQD82_001175"
     CDS             8268..8600
                     /locus_tag="KQD82_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379653.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8720..9361)
                     /gene="pcp"
                     /locus_tag="KQD82_001176"
     CDS             complement(8720..9361)
                     /gene="pcp"
                     /locus_tag="KQD82_001176"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG4379654.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9382..10314)
                     /locus_tag="KQD82_001177"
     CDS             complement(9382..10314)
                     /locus_tag="KQD82_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG4379655.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10336..11001)
                     /locus_tag="KQD82_001178"
     CDS             complement(10336..11001)
                     /locus_tag="KQD82_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG4379656.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11548..12024)
                     /locus_tag="KQD82_001179"
     CDS             complement(11548..12024)
                     /locus_tag="KQD82_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG4379657.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(12027..12431)
                     /locus_tag="KQD82_001180"
     CDS             complement(12027..12431)
                     /locus_tag="KQD82_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG4379658.1"
                     /translation="MKIRNKATNKKKKYVRISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGGADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12406..13035)
                     /locus_tag="KQD82_001181"
     CDS             complement(12406..13035)
                     /locus_tag="KQD82_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4379659.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13400..13443
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13544..14512
                     /locus_tag="KQD82_001182"
     CDS             13544..14512
                     /locus_tag="KQD82_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG4379660.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFISMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14514..15077
                     /locus_tag="KQD82_001183"
     CDS             14514..15077
                     /locus_tag="KQD82_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG4379661.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15085..15774
                     /locus_tag="KQD82_001184"
     CDS             15085..15774
                     /locus_tag="KQD82_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4379662.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15901..16629
                     /locus_tag="KQD82_001185"
     CDS             15901..16629
                     /locus_tag="KQD82_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG4379663.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKKFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16643..17317
                     /locus_tag="KQD82_001186"
     CDS             16643..17317
                     /locus_tag="KQD82_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG4379664.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17343..18179
                     /locus_tag="KQD82_001187"
     CDS             17343..18179
                     /locus_tag="KQD82_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379665.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18195..18866
                     /locus_tag="KQD82_001188"
     CDS             18195..18866
                     /locus_tag="KQD82_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379666.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18856..20088
                     /locus_tag="KQD82_001189"
     CDS             18856..20088
                     /locus_tag="KQD82_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379667.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20238..20555
                     /gene="trxA"
                     /locus_tag="KQD82_001190"
     CDS             20238..20555
                     /gene="trxA"
                     /locus_tag="KQD82_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG4379668.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20572..21450
                     /locus_tag="KQD82_001191"
     CDS             20572..21450
                     /locus_tag="KQD82_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG4379669.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTMMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21720..22088
                     /locus_tag="KQD82_001192"
     CDS             21720..22088
                     /locus_tag="KQD82_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG4379670.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22091..22462
                     /locus_tag="KQD82_001193"
     CDS             22091..22462
                     /locus_tag="KQD82_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG4379671.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22606..23061)
                     /locus_tag="KQD82_001194"
     CDS             complement(22606..23061)
                     /locus_tag="KQD82_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379672.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23100..24365)
                     /locus_tag="KQD82_001195"
     CDS             complement(23100..24365)
                     /locus_tag="KQD82_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG4379673.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            24835..26286
                     /locus_tag="KQD82_001196"
     CDS             24835..26286
                     /locus_tag="KQD82_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG4379674.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(26506..26967)
                     /locus_tag="KQD82_001197"
     CDS             complement(26506..26967)
                     /locus_tag="KQD82_001197"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG4379675.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(27072..28265)
                     /locus_tag="KQD82_001198"
     CDS             complement(27072..28265)
                     /locus_tag="KQD82_001198"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG4379676.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(28288..28953)
                     /locus_tag="KQD82_001199"
     CDS             complement(28288..28953)
                     /locus_tag="KQD82_001199"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG4379677.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(28987..30099)
                     /gene="ribD"
                     /locus_tag="KQD82_001200"
     CDS             complement(28987..30099)
                     /gene="ribD"
                     /locus_tag="KQD82_001200"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG4379678.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(30231..30352)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            30541..33024
                     /gene="recQ"
                     /locus_tag="KQD82_001201"
     CDS             30541..33024
                     /gene="recQ"
                     /locus_tag="KQD82_001201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG4379679.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAAIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      33113..33224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33366..34667
                     /gene="thiC"
                     /locus_tag="KQD82_001202"
     CDS             33366..34667
                     /gene="thiC"
                     /locus_tag="KQD82_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG4379680.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            34721..34915
                     /gene="thiS"
                     /locus_tag="KQD82_001203"
     CDS             34721..34915
                     /gene="thiS"
                     /locus_tag="KQD82_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG4379681.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            34908..35711
                     /gene="thiF"
                     /locus_tag="KQD82_001204"
     CDS             34908..35711
                     /gene="thiF"
                     /locus_tag="KQD82_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG4379682.1"
                     /translation="MVKIKIFVNELLTDVEENITAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            35774..36544
                     /locus_tag="KQD82_001205"
     CDS             35774..36544
                     /locus_tag="KQD82_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG4379683.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            36561..37664
                     /gene="thiH"
                     /locus_tag="KQD82_001206"
     CDS             36561..37664
                     /gene="thiH"
                     /locus_tag="KQD82_001206"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG4379684.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            37665..38354
                     /locus_tag="KQD82_001207"
     CDS             37665..38354
                     /locus_tag="KQD82_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG4379685.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            38542..39936
                     /gene="yfcC"
                     /locus_tag="KQD82_001208"
     CDS             38542..39936
                     /gene="yfcC"
                     /locus_tag="KQD82_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG4379686.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            40453..41820
                     /locus_tag="KQD82_001209"
     CDS             40453..41820
                     /locus_tag="KQD82_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG4379687.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            41845..42453
                     /locus_tag="KQD82_001210"
     CDS             41845..42453
                     /locus_tag="KQD82_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG4379688.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            42501..>42871
                     /locus_tag="KQD82_001211"
     CDS             42501..>42871
                     /locus_tag="KQD82_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG4379689.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
BASE COUNT        16450 a         4773 c         6879 g        14769 t
ORIGIN      
        1 tataattact aaataaaata aattaatcaa tatagttttt taatataaac aaaaaataaa
       61 aaacttatat aaataaaatg cttgtttttc ttgtattatt tatataaata ttgaaatttg
      121 tctagttata cgtttttatt gaccagttat gcagttgata ttgaatattc taaatattgg
      181 tgttatttta taggtggttt aaacatatta gggggatttt atatatgatt tgtatgattt
      241 atagagaatg tataaaatag taaaggttta gagccatgtt tagtggattg cagaatatgc
      301 atatagaatg attcttgtaa attaagttag acataggaaa tatataattt gtattggatg
      361 attgatttta tggtgtatgc ttttaacttc tataatggga aagtaaagat gtactcccaa
      421 tacatcttta ctttagcaga agttatagtt acttataaag tacaaaaaat tatatattta
      481 ttagagtaga taaatctaaa aaggtttttc tagaaaaatt tagttttaat tgtatctact
      541 tactaataat aagacttgat gtaagcaggt ttcaactacc gtttctccat attctgatat
      601 aaaatcatct aactgtgtat ctaaaaaatt acaataatca tatgaactac tgatgtaagc
      661 agttagtata tgtgtacatt tgtctatttc tccaataaac atattatcca tccccctaaa
      721 aaatttagta aaaataaaaa gtcgcattct attagtcaat taacataatt tataaaattt
      781 tttagtacta gtttctataa cttaactcct taaggttaaa tattcccagt tttaccaatt
      841 tagttttatt ctacaagtat ataatactag aagatgcttc attattcaac atgcttgttt
      901 gagaatagaa aatattctaa aattataatt gtttttaaaa ataagattca agataaataa
      961 agttatatgc aactatttaa taatctttcc ataataagga cagtaggttc tattcttaaa
     1021 aaactccatc tttaaattta aagaataact acaatttgaa caacaataaa atttccaatc
     1081 aataaaaata tcaaaaataa taaaaataat tgaaaatgta taatttccca gaaaaataat
     1141 aaatgtaact ccaataataa acacacctat aaattataaa ataatttaag ctttatttaa
     1201 ggttagtatt atatttatat aagatttaag ttttaatata tatgtgtaat taaaatgatt
     1261 taggaggaga aatatattgg taaattggga aataaaaaaa ttggctaaat ctaagagtat
     1321 gtttatatct ctaggcatat tagtgttaat actaatgatt agtatcttta ttgcgcctgt
     1381 tttagaaaca gaaagttatt atatagataa agaaaaagga cgtatagaag atacgagaag
     1441 tggtatcgac ataggtaatg agaaatttca aaataagatt aatgttttaa agcagttttc
     1501 aaatcaaaca gatacaggtg aattttctaa aaaaattgaa ttaatgtcaa agaaaaaact
     1561 tgataatcta agtgtgaatg aatataagga tattagtttt tggaaagtat ttaactatag
     1621 agttacaaat tcacttataa atgtaggaat gttaatcatt atatctataa ttatatccaa
     1681 tatttacgta gatgaagtcg ttacttcggt aaaagatatc atactatcat caaaagagaa
     1741 gaaaaaagca ttaaagtcta aaatatttgt tgctttatta gttccattag taatttattc
     1801 tgtttattta atgggaatat ttattataac ttgtatgcaa caaggagtac ctgttaatgg
     1861 agacttagaa tcttttagaa tagtagacaa tgtagttgca ttgaaaacta atttaagtat
     1921 aacaaagtat gtaatattaa atataggcat agagcttttg atgtttgaag gatgggccat
     1981 gatagccata tttgggtctt ttataagtaa gtcttcaata tcttcaataa gtttttttat
     2041 atttattata tctataacta agataatgtc ggtaatccat atattaccta aaaaattact
     2101 ttcagttgta tcaaatgtaa attattatga tttgatattt ggttttaata aaataatagg
     2161 aaattatcta ggtgatatta tgatatttaa agtgaatata gacattgtca atatagcaat
     2221 aggacttttg atggcaatat tgataggtac tatgtcatta tgttttttag taataaaatc
     2281 agattatata agtagataaa aggggagtta agatgaataa attagaaata gaaaatcttt
     2341 caaagatata tggtaaaaaa gtagcaaacg acaagattac agtaactctg gaaaatggag
     2401 tttatggtct tctaggacca aatggagcag gaaaatctac attaatgaaa caaataacaa
     2461 cgttgataaa acctgatgaa ggtcagatac tttataatgg tgaggatata tttaaaatgg
     2521 atgctgatta tagaaatatt ttaggatatt tgccacaaga atttggagtt tataaaaatt
     2581 ttacagcaaa agaattttta cagtatattg gagctttaaa aggtttaagg ggaaaatatt
     2641 taaatagtaa gataggagaa ttattagatt tagtaggtct ttatgatgtg agaaataagt
     2701 ctataggaaa attctcagga ggtatgaaga ggagagttgg gatagcacaa gttttactaa
     2761 acgaccctaa gataatagtc cttgatgagc caacagcagg tcttgaccca caagaaagag
     2821 ctagatttag gaatttaata gcaaaaatgt ctagagataa gataataata ctttctactc
     2881 atataatttc cgatatagaa tcagttgcaa aggaaacaat aatgattaaa gatggcaaac
     2941 tcctaatgaa aggaacacac aaagacatat taagtcacat ggaaaataaa gtatataatg
     3001 ttcaagttaa aaatgaatgt gaagttgatg atatacaatc taaatataag gttgtaagta
     3061 ttcaaagtga tattaattca atagtactta gaattgtttc agaggatatt ctaagcgagg
     3121 ataatataca accagttcaa gcaaaattcg aagatgtata catgttttat tttgatttag
     3181 aagatgcaag ggaggtgtag tagaaatgat gtatttagtt aaagctgaaa tgataaaaat
     3241 attaaaatca aagagtgttt ggataatttg gctgttttta atattttttg gattttttct
     3301 gattagaaag tttgatgttt tagatactta tgcagatata ttttacaaga tagaaggctc
     3361 aatacctcta ataggtttga taatgtttat aataacttct ggaaattaca ttaaagaata
     3421 tgattctaat atgacaggtt taataaatac aactaaaaag ggaaaaaaat cagtagtatt
     3481 atcaaaacta gtagctaatg gattaatttt atctattatt aatatttctt ttatattgtt
     3541 agtaggaatt agaggtttta gtttttttga ttttaaaaat ttaaatgaac cgattcataa
     3601 cttatggtac tttggaaata gtaatttaaa tcttacagta atacaaatgt atataattgt
     3661 tatattgact actatttttg cttcttttat atttgcacaa ataggcttaa cactttcatc
     3721 tatatttaaa tcggctgtaa ttccatttat actaggtgga ttgataatgg caattccata
     3781 ctttagtgta ggttttatac cagataaagc tataaaattt atgtcagtga ctccaaattg
     3841 gattatgatg agccaacaaa tggttaagta taatgtacca agtattttga tagttttttc
     3901 tatagtaatc tcaattatat taatgatagt gttaactaaa ataacatatg aaaattttac
     3961 atcaagcaaa aggttttaac taaaaataat agtaatttta tttatatttt agtttaatta
     4021 aaaataagat aaatgaattt tagataaaat atatgctaat atatatgtaa attattacag
     4081 gaggttgttt atggaaaatc gtgttttaat aatagatgat gaagtagaaa tattgaagct
     4141 tttagaaact gtcctaaaaa aagaaggcct taataatata tatactgcaa aaactaaaaa
     4201 agaaggatta gaattattta aaagtattaa tcctgatttg attgtattgg atattatgtt
     4261 accagatggc gaaggttatg atatttgtaa agaaataaga aaaacctcca attctccaat
     4321 aatattttta tcagctaaaa ccgaagaatt agataagtta cttggactag cgattggagg
     4381 agatgattat gttacaaaac cttttagccc taaggaagtg gcttttagag ttaaagcgca
     4441 tttgagaaga ttaagttatt ttagtgatgc tcaaaacgaa agtaaaaatc taaataataa
     4501 tgaagaaaaa attatatcat ttggcccata tatattaaat gaaagtaggg cagaacttat
     4561 aaaagatgga aaatctatag gactgtctgc aaaggaacta aaaatcctat ctttatttgc
     4621 acataatcaa aatcaaatta taagtaaaga aaaactatgg gataaagtgt ggggagaaga
     4681 ttatgttgga tttgacaata ctataatggt tcatataaga aaaattaggg aaaaattaga
     4741 ggataatcct tcaaaacctg aatatattct gacaataaaa ggtcttggat ataaattagc
     4801 agtaaaggaa gattagaata tgaagtggaa gattactaga aattttatat ttacaattgt
     4861 gtttgtagct atatcagtag taattataaa tatcatatct atattgtatg tgatttcaac
     4921 taatagcttt tttaaagttg tagattctgg aaataatcct gaagaatttg cacgttcttt
     4981 tgaaaaagat ttatatgaaa aagatggaga atttaaacta tctaagattg gagctgaaaa
     5041 attagaaaaa tcaaattcct ggatacaggt tttaaatgat ttaggagagg aagtttatgg
     5101 agtaaatgtg ccaaaggata ctccaaaaaa atatacaccc tttcaaatgg tgaataatta
     5161 caagtatata gaaacaaagt atgttaattt tgtactagaa aaatatttaa acaaaaaaca
     5221 tctaaacata atagtaggta tacctagtag agatatatca agaataattt taacctattc
     5281 tcaaaataac attaaaaaaa ctttaaataa agttatcatt ataacactag taattgatag
     5341 cgtggtagct ttaggcgtag gatatttatt tagtagaaaa cttacaaaac caatttcaag
     5401 cgtactatgg agtattgaga ctatggctaa tggaaattat agtttatatc ttaaggacag
     5461 aggtatatat gaagaagttt ttaaaaatat aaatatgtta gctgatactt taagagtaaa
     5521 tgaagttgag agaaaagaaa atgaagagtt gagagaggaa tggctggcaa atataacaca
     5581 tgatataaag acacctctag catctataca agggtatgca gagataatta atgacaaaga
     5641 ttatgagttt gaagaagatg aaatacaaga gtatacagaa attatatata ataagtctaa
     5701 gtatataaaa gatttagttg atgatttaaa tttatctact cgattaaaaa ataatactat
     5761 agttctggac aagaaaaaaa taaatttagt cagtttagtc agagatataa taattgatat
     5821 tttaaatgat aatagatata aaaataggaa tattgaattt gaaagtaatg aagatttaat
     5881 tgaagtatat gtagattcaa tcttattcag aagggctata accaatctta tatttaattc
     5941 aatagttcat aatagcgaag gaactttgat aagtgtagaa attgtaaaaa aagataatat
     6001 agaaattata ataaaagata atggaattgg aataagcaaa agtgatttga aacatatttt
     6061 taaaaaatat tatagaggta ctaatacagg tgagatgcat aaaggttcag gtcttggtat
     6121 ggctatttct aaagaaataa tagagattca taaaggtaaa atttatgtaa gtagtgaaat
     6181 tggaatagga acaaaaatta ttatagagat aaaacaaaat taggagatat gatatggatt
     6241 tttttaaaaa aggtataagt gggtttacta ttaagatttt agcattaata tttatgacat
     6301 ttgaccatat agcagcattt atgccacaaa caatgcaaat accaatttgg tttcattggg
     6361 taggcagaat atcagcacca ctgtttatat ttatggctgt tgaaggattt tatcacacta
     6421 gtaacagaaa aaaatatata agtagattgt atatttggtc tgttattatg gctataggaa
     6481 atcaaataat taataatgta ttttcacatc cagaaggagc tataattata aataacattt
     6541 tttcaacatt atttttaata gcgatttatt tacaagcaat agagttcata aagaaattta
     6601 gaaaagagaa agaaattaaa tattttatta taggattatt gatgataatc attcctataa
     6661 ttttaggaat atttacagta gctttacttt ttaaagtcac taacagagtt attgctttat
     6721 ttatgatttt ggtaccagta ccatttttag tagaaggtgg accaatttgg attatacttg
     6781 ggattatttt ttatttgtgt agagggaaaa agttcagttt gagtatttgt tatgctttaa
     6841 tgtgtatatt tatatttaca acaatgtcaa atggagatta tagtttaaaa aattctattt
     6901 tacaaaatta ccaatggatg atgattgctt ctctaccttt aatgttactt tacaatggag
     6961 aaaaagggaa gagtatgaag tatttatttt atttatacta tccaatccat gtatatatat
     7021 tgtatatctt aggaatatac ttaataaatg gtttttgatt gtaagtgata tttttaatac
     7081 aaaatgaaca atatgaggat tgttttaggt ctattaaaat tgttatttag atgagatttg
     7141 tagaatattt aaatttatga gcagaaattc agttattgta atttcaataa cattagaata
     7201 taatattata atttatttag aaatatatga tgttatacat ggtttggtta ttaatgtaat
     7261 aataaaacag aggtttgata taaaattcaa tacctctgtt ttagtgcttt ttatcctaca
     7321 tttatgctta ttttatttat aaattagtgt tttgttttaa ttaatattag tacatttttt
     7381 atttatttta taaaattagt cataattgtt tcttcatatt gtggttcttt aattccagct
     7441 tttttacctg ctgatataca cttaagaagc catgccatgt tttctcctag agttcgcata
     7501 gtctgtaatc cttcttcatc cttcataaca tcctctggag taaagccatg tacctggttc
     7561 caatactgtg atgatacaat aggcatattg ctgattgaaa aatatttatt tagttggtca
     7621 aatgttgcac ttgcaccacc acgtctacat gacacaactg aagcacctaa ttttgtagcc
     7681 atgcgacttc cagctatgaa aaatagacgg tcaagaaaag aagttaattg accagttgca
     7741 ctagaaaagt aaacaggaga accaataata ataccatcaa tattatccag atcttcaatc
     7801 aattcattaa ctttatcatt tcgaatgcat tttccagttt caaaacagga tgcgcaacta
     7861 atacatcctg ggattgtttc tttacctaaa tacagtattt ctgttttaat atcgtgttta
     7921 ctcaatactt tagtaatctc atttagtgct gtataagtac atccatattg gtttgggcta
     7981 ccgtttatca ataaaacttt cattataaat ttcctccaat gaattttatt taatagattt
     8041 tctaagagat attatttttt ataaaatagg attttcatga atattatcaa tttatataga
     8101 aatgtctaat cttagaatcc tatttaaaat aacttttttt tagtaaatac acctcctatt
     8161 ataaaattga ctttcaatta ttcttacact ataattttat tataaattat tctaaaaaca
     8221 agtacgcaca tttttgttca atactaacca aaaggagagt gtaatatatg agtatagact
     8281 gtatatcaaa tacagattta agtgaaacag gatttagtta tacactttca ctgattagtg
     8341 gtaaatataa gatgataatt ctttactgtc ttgtagaatt tgaagttgta agatataatg
     8401 cattaaaacg ttatataaat actatctcct ataaaacttt gagtttatca ttaaaagaat
     8461 tagaagcaga taacttgatt attcgtactg aatatcctca aatacctcct aaggtagaat
     8521 atagtctatc agaaagggga aaatctctga ttccaatttt agatgcaatg tgtgagtggg
     8581 gagagaggaa tagaccttaa ttttgtatct gtagatacca taaaaagact gtgagtaaaa
     8641 ttgtttgaat aatctactca cagtctatat ttaaattaat agttagtatt tttaatttat
     8701 aaaaatacta aaaaatttat tatgaaattt caccaccaat ttcttttaca tcttctttat
     8761 atataataca agcttctata gctaattcta agccttttac tatttcttct aaagacatga
     8821 atggtgtatt ttttttatct attacttgtg aagttgtata tgggatgtgt ataaatccac
     8881 cccttatatt aggatatttc ttgtctatca tataaagaat tccatacatc acatgattgc
     8941 aaacaaatgt tcctgcagtg tttgaaacac tagctggaat tttattttca ttcatatgtt
     9001 ttaccatagc ttttacaggc aattttgaaa aatatgcagg ttcaccatct tcttttataa
     9061 tagtatctat aacctggtta ccctcattat cttttattct aaaatcatct atatttatag
     9121 caactctctc aggtgtaatg tcaaatctac ctccagcttg tccaactgat attactatat
     9181 ctggattgtg ctcttgaata gctttatcta tagcttcaac agattttgtc ataactgttg
     9241 gtatagttat ctttatgatt tctgcaccgt ttatattatt attgaccctt tcaactgctt
     9301 cttgagctgg gtttattggt tctccgccaa atgggtcaaa tcctgttaac aaaattttca
     9361 ttattttacc tcctaattat attaaaatcc taaaaagtac attactaata tgtgggctat
     9421 aattaatacc aaagcaactg gagcttgata ttttataaca atataatcat ttttacattc
     9481 tagcaaggct gctggaacta tattaaaatt agcagccatt ggagtaagta gagttccaca
     9541 atatcctgct gttaatgcta atgctccaat tatagcagga tttccacctt gagacaatac
     9601 aaaaggaata ccaattcctg ccgttatgac tgaaaatgcg gcaaatgcat ttcccattat
     9661 catggtaaat ataaccatac ctaatacata agctataaca ccaaataatc tgctatcagc
     9721 agggactaca ccagatatca tggttgaaat aacttcaccg actccggctt gagtaaataa
     9781 agctcccaat gcaactaaaa gttgtggtaa catactagca ggtcccattt gttgaagtaa
     9841 tcttgagcca tcttgaactg atgtagcagg ttttgcctta gttattataa gggctgatat
     9901 taatgcaatt attgaaccca cacctattgc aaattgagaa gaattatcaa aattagaaag
     9961 gctcatagct actactactg cagcaaatgc taatattaat gatggtataa atattttatt
    10021 tttaagtttg tctgcttgtt cttttccaaa attttcatct ggttcttcat actttgcaaa
    10081 tacaacttgt ttagaaagtg ttaataaact agataatatt aatattattc ctattatatt
    10141 ggctggaatc aatctaccaa atatgaatgg caatgatata agtatccaaa ataatgatgt
    10201 accatatttc ttattgttat ttatggtagt aaaagactta tatgctataa caatcattaa
    10261 aaatcccatt agtgcgtaaa agatttctaa tgatatgttt ataaaatttt gcatttttat
    10321 tcctcctaaa taaaactatt ttttatgact tttgtttaat tttttatcat ataaggtaaa
    10381 ctggattgta gccactacaa gtgctattac tgccataata gtactagcct ttacaatatt
    10441 aagtccagtt acattgtatc caaattgagt cagagtagat gctataagta atactccact
    10501 actacctgca aataagtttt gaccataaaa atttccatag ttttctgatg cagcagataa
    10561 tgcctttatg gcttcttcat ccgattcttt tagttcatca ctattactta aaccagcagc
    10621 ttgtgccata ggattaacaa gtggtcttac aaattgagga tgtccactca ttcttattga
    10681 tagagcacca gctatttgtc tagctatcat gtatatagta agtacttttc ctgttgtaag
    10741 tctttttaat ttttctatta aagaaacagc tctttgtttt aaaccatatc tttctaatac
    10801 accgattact ggcaatgtta atatgaataa tgatacacct ctgtttgaga caaaagaatc
    10861 tcctaaaatt gtaaaaattt gattaaaatc taatcctgca acaattcctg ttgccactcc
    10921 tgctaataat acagtaaata gtgtgtcaat tttaagtata aatccaatta caactatcaa
    10981 tataccgatt agttttatca taaattgttc tccttccatt tctatatgtt ttaatttaaa
    11041 tttatttaat ggataaagtt aaactctttt tatagtattt gcgttaatag gactattatt
    11101 ataaaattta ataaaaaata gggaagatgt ttttaagtat caaagtaaat aatggttgtg
    11161 tataggaatt tgaataacaa agattatatt ttaagctaat atgaattgat tttgtaagtc
    11221 tatagtttaa gtatatgtca atatttgaaa taggttttaa attagatatt atattataaa
    11281 aagagaatgt gccaaacata gagacaagtg tatttttcac ttttgaaatg ataaaaatat
    11341 atttgatttc atacaaaaag agagtgtcta atgaattttt tagttcatta gacactctct
    11401 ttttgttatg attaataaag catatattaa gtgctagttt cattagattt cactaagaaa
    11461 aataaaattt tgataaagtt taaagactat tttgagcaat attacttata aataaaagaa
    11521 tttatcatag agataattag ttttatttta gctattaaga tttattaaaa aattatctat
    11581 attatcctgt gatattctaa aactgtcata attacctttt gaaacagcct tcataataag
    11641 tttatctaaa aatttcattt tttctaatcg agcttcacta ccaaaaattc caatagcatt
    11701 tgcactttct aataaggatg atggaaaatt tgattttaaa tatttatctg cttgttcaga
    11761 aaagccacag caaaggaaaa tgccaacttg ttttttgttt aataactcta cattatcatt
    11821 acatagcact tgtatttttt ttgaaactga accaacatat atagaacttc caataataat
    11881 tttatcaaat tttgataact caattttatt attattatta ttaatatcaa caatagtgac
    11941 attatctgat agtttatttt ttagtatatt tgcacagtct tttgtacaac catatttact
    12001 tgaatatata ataatagtat tcataattat tctccctttc ttaaagcatt ttctacagct
    12061 tttcgtatag tattactgct ataagttgca gaagttatag catccacatc taaagactgt
    12121 tgtttaataa tacttgttgt aattttttca gcaggtttac ctaaaagatg gtcgtgtttt
    12181 aagatattaa tattttggat tttaccatta ttaacttcaa cagacacagt agcttttacc
    12241 aagttattat cagcacctcc agtataaatg ccatttttaa cattattaat atctatatct
    12301 tttactacca agttttccgg ctttaagttg cttacaatta caataataat gcatataaat
    12361 acaaagaata gagcaataga tattcttaca tattttttct ttttattagt agccttgttg
    12421 cgaattttca ttgtaaagtc ctcccactaa aatatctaag aaattttcta taagttgaga
    12481 aaaattatgt aaagaatata gtttattttc aacaacacat aaaacgaatc ctaacaatgc
    12541 acttataagc atccatgagc ttttatctat attttttttg aatattcctt gttcaattcc
    12601 aatggaaagg aatttatcaa gcatttttat accattattg ctaggcgtat tgtttgtatt
    12661 ttcagatgca aatataatat tcattctact atgcatgata gattttgcca tctctggttc
    12721 actagaaaga gtttttataa agctagtcat aatttctatg atttgtttat ctattggtag
    12781 tgatgagcaa ttattcataa tgtcaatggc attactttct actgtgttat aaagtttatt
    12841 ggtcatatcg ctgataattt ctgctttgtc tttgtagtaa agataaattg tagttggaga
    12901 atattctatt tttgtagcaa tttttcttat tgataaattt tcatatccat gttggtttat
    12961 aatttcgatg ctagcatcca taatcttttt cttcatttct tctttttcat ttttacgtct
    13021 ttctttgata ctcatataat tacctctttg cttaacggtg ttaagttaat ttacactgtt
    13081 attgtataat ttaattttaa tactgtcaat aaattattaa ataatataag ttaatatatt
    13141 tttatggata ataaattaat gttattaatt gtaatattta agatattatt gtataataat
    13201 aatcggttta aatgcgccac atcttattaa agttggttca taattctaaa taaactttaa
    13261 taagatacac taaaatataa taaattcttt tatgctataa aatttatttt atagctatta
    13321 aatcagatgt ttaaaaattt tttaacggtc tatagttttt aagagtctat agtaagtaga
    13381 ctaaaaatat gattttacat ctgtacttgt tcgagaactc caagtataaa aaactaagga
    13441 attatatagt ggcagtatat aattaatacc cttagacaca cctttttgaa agtggaaaaa
    13501 agatgtgtag ttctcctcga ccacaaatca aggaggataa aaaatggaaa agagaaaagt
    13561 aataattgat tgtgacccag gaattgatga ttctttggca attcttctgg ctttaaactc
    13621 accagagcta gaagtgattg gaattaccac atgttgtgga aatgttccag caaatatagg
    13681 tgcagaaaat gcactaaaaa cacttcaaat gtgttcttca ctaaatattc cagtatatat
    13741 aggagaagaa gcaccactaa aaagaaaact tgtaacagct caagatacac atggagaaga
    13801 tggtattgga gaaaactttt atcaaaaggt tgtgggagct aaagcaaaaa atggagcagt
    13861 ggattttata ataaatactt tacataatca tgaaaaagta tcaataatag cacttgcacc
    13921 acttacaaac atagctaaag cacttattaa agataagaaa gcatttgaaa atctcgatga
    13981 gtttatatct atgggaggag catttaggat tcatggaaat tgctctccag tagcagagtt
    14041 taattattgg gtagacccac atggagcaga ttatgtttac aagaatttat ctaaaaaaat
    14101 ccacatggta ggtttagatg taactagaaa aattgtactt actcctaata ttattgagtt
    14161 tataaataga cttgataaga agatggcaaa gtatataact gaaataacta gattttatat
    14221 tgatttccat tgggaacagg agggaataat tggctgtgtg ataaatgacc ctctagcagt
    14281 agcatacttt atagatagaa gtatatgtaa aggatttgaa tcatatgtag aagttgtaga
    14341 agatggaata gctatgggtc agtctatagt ggattctttc aatttctata aaaaaaatcc
    14401 taatgcaatt gttctaaatg aagttgatga gaagaaattt atgtacatgt ttttaaagag
    14461 gctttttaaa ggttatgaag acattataga ctctgtggaa ggagtgatat agtgtgaaaa
    14521 gaaaaatatg tgtaaaaact attacactta tagcttttgg tatttctata aatataatag
    14581 gcgcttttat agctatggga ttaagattac ctgtatactt agattcaata ggaactataa
    14641 tgatagcttc acttttagga cctaaatacg ctgttgttac aggtgtgttt ggaagtttaa
    14701 taagtggaat tacgtttgat gtgtattctc tctattttgc accagttcaa atatcaaccg
    14761 gattacttgc tggaatagca tttaaaaaga attttttaag aggtttaaaa actccaatag
    14821 gagtactttt atttgccatt ccaacatcta taataagttc aataatttca gcatttttat
    14881 ttggaggtat gacctcttct ggttcatcat atatagtgca aatcttgaaa gtgttaggac
    14941 tgggagatgt ttttagtgtg tttgtaaccc aagtatttac tgattatgga gataagctgt
    15001 tagcagttgt tttagtgaat ttaggattaa atgcagttcc aaaaacttta aaggtatcat
    15061 tgactggagg aaaatagata gaaaatggat agatatagtg agataacaaa taaaaatcaa
    15121 agagaaattg ttctgcttaa aggatttcct tgtatatggg gaaagtgttc tttttgtgat
    15181 tatatagatg ataattctaa tttagaagaa gagatgaata aattaaatct aaaggtttta
    15241 aaaaacgtaa ctgggaaata tggagttctt gaagtcataa attctggaag ctgttttgaa
    15301 ttacctaagg atactttaga aaaaataaag tgtattataa aagagaaaaa tataaaaaaa
    15361 ttatttttag aaagtcattg gtcttacaaa aataggctta aggaaatgag agagtatttt
    15421 gaaattcctg tggtatttaa aattggagtt gaaacctttg acaatgattt tagaaataat
    15481 attttaaata aaaatgcaaa ttttaagact ccacaagatg taaaagaata ttttgattca
    15541 ccttgtatta tggttggaat aaaaggacaa acaaaagaga tgatagataa agatatagag
    15601 attatcctaa atacttttga aaaagcgaca gtgaatgttt ttataaataa ttcttctagt
    15661 ataaaaagag atgaagagtt agtgaaatgg tttgttagta agtataaatt cttggatgaa
    15721 aatccaaata tagaagtttt atacaataat acagactttg gtgtaggaga ttagcattaa
    15781 tttagtttaa atttaaaatt gaaaagtttc ttatgttttt gaaaaagctt ctaatagtta
    15841 gaagcttttt ttgtagagtt ttatgttaca ataagaaaaa aggaattaag gagcaattaa
    15901 atgatgtttg ataggggaaa atggaataaa ttggattata atgaactttt agaatatatg
    15961 atgtctatac aagatataaa atatagagat tttaataaaa agcttatacc tggaacagaa
    16021 aatataatag gtattagagt tcctaattta agaaagttat caaaagaaat aagccaagga
    16081 aattggaaga aatttttaga agtagcagag gatacatact atgaagaagt taggctacaa
    16141 ggtatggtaa ttggcaatat aaatagtaat tttgaagaaa ctttatacta tgtaaaaaga
    16201 tttataccta aaatcgataa ttggtcagtt tgcgatggat tttgttctga tttaaagtct
    16261 atgaaaaaat ataaagaaaa tatgtatgat atacttaaaa aatatgttta ttcaaagaat
    16321 ccatgggaaa ttagatttgc attagttatg tttttaatat attatgttga tgataaacat
    16381 attaaagaaa tatttgaata ctgtaataat atacaaagtg aagaatatta tgttaaaatg
    16441 ggtatggcat ggttgctatc aatttgtttt attaaatgtg aagaggaaac ttttctctat
    16501 ataaaaaata ataatttaga tgatttcact tataataaaa tgctacaaaa aataattgaa
    16561 tcaaatagag tagatttaga aaagaagaat ataataagaa gtatgaaaag aaagacaaga
    16621 agatgttgac ggaggaatat taatgaataa caatgaagac agaattgaca aatttttaga
    16681 acttcaaagc aaaggattta aatttgcaga gatagctcaa aatctaaaga ttagtcaaag
    16741 tactttaaga acttttttaa ataaaaaagg atataagtca gaaaaaggta aatacatatt
    16801 aaaagacgaa ttaaattata ataaaagttt ggataataga aacttggaag aagagaaagc
    16861 taaggagtat gaaaaaagta aaaagaatac tataagtaat agaaaaaaag taagcaccaa
    16921 aaaaaagatt tctaatgaaa aggctttaag taaaagtgtt gaacaaattg agtttaatga
    16981 tacaggctta aaaaatgcaa agccaaaaca aaatattaag cctaagaaag atagaaaaat
    17041 aaatataact caagaagatt tagataagct ttgtgaagta tatgattggt atttagaagt
    17101 aaaagattac aatataaagc ctaaaaaagt atctaataaa aaagatgtac atatagatga
    17161 tgaacacata gatgaattaa aaacaacaag aatacgtata gataaagaaa tatggaatga
    17221 ttttgaaaga ttatgtagta attcgaattt tgaaaaacat caaatactta cacaggcatt
    17281 gaaagaattt atgaaaaagt ataaaaatct actataaaat actactatat gaggtgctta
    17341 atatgaataa aatagtaaaa aaatcaattt ctctaatgat tatattaaca atatttattt
    17401 ttatgttgac agcatgtgaa aaagatgaac agcctgattc agttgatatt caaacagaag
    17461 ataaaaatga gataaaaata gatgagggaa atgcaaaagt attgaatata ggaaaatcag
    17521 aaattataaa tatagatgaa ggtgataaag tagatacatc aacaaaggta gaaaataatt
    17581 caacatttaa tatatccaat gttgaattga tatataatga atatgatgag aataaaaaaa
    17641 ttatatcttc ggatagtaag gctttgttgg acatgacttt aatgcctggg aaagttgctt
    17701 acatagaatg tgggcataaa acttttgtaa agggtgtaga agtttatgca tatgaatatg
    17761 aggcagaggg taagattgtg tatgttaatt taaaagaaaa tactattaat ataagaaata
    17821 ataatattaa gctagaaaat agtagtcagt atgaggtgct atctacatca gaacttaaaa
    17881 aagtaaatga atcaaatgag ggaattacgt atcaagtaaa agtaaaaaat tcatcatcaa
    17941 aagatttagg aaatattatt cttaagacag cagaagttaa tgaaaatgga gaatatttaa
    18001 cagtaaatcg tgtgccatca tataagattt taaagccttc agaagagaca gatatagata
    18061 tattgtgttc aacaaaggca aaaagtgttg aaatagtggg atatacgtat gatgatataa
    18121 aagaaaaagc caatgtggat attgacttaa aatctcataa agttaagata gacaaataaa
    18181 ggtaggggat acttatgaag gttttaatag ttgaagataa taagatactt ttagaaagtg
    18241 tagtagaaga acttagcaag cattttgaaa ctgaaaagtg tgaagatgga gaagaagctt
    18301 tatacttagt aaatcaaaat atatatgatt tagtcatttt agacttaatg cttcctaata
    18361 taaatggatt tgatatccta aagaaaatga gagttaataa tatagataca cctgtactta
    18421 tattgacagc aaaagaaaca ttagatgaca aagtagaagc atttactata ggagctaatg
    18481 attatttgac aaaacctttt tatatggaag agttagtagc tagggtatac gctatattaa
    18541 ggactaatgg aaaaataaaa gaaagaaacg gattagaatt taaatcttta tatttagata
    18601 cactagaaaa aagagtgtat attgaaaaag aagaaataaa acttcaaaac aaacagttta
    18661 acttgcttga gtattttgta ttaaataaag gttcaatttt attaaaagag cagatttatg
    18721 atagaatatg gggaattgat tcagatgcga ctattgaaat agtggaagta tatgtaagta
    18781 atctaagaaa aaaactaagt aaatatggat atgataaata tattaaaact aaacgtaagg
    18841 taggatatat attcgatgat aaataagaat gtatttacca gtactaaaaa tcatttaata
    18901 aaaatgtata ttattgtagt aggtagtttt ttaataatct tttctatatt tatatactca
    18961 tattttaggg gacttacata tagtggaatt gatagtgaga taaatgatga acttgaatat
    19021 atagtctctc aatttaaaag aacttcattt ttgaacccaa taagactgaa agatcctaaa
    19081 gatatggtgt atgtatatga agatggaagg ataagttact atactcaaaa tgaatatttt
    19141 gatgagttac ttccagatag aagactagat aaaaaaaatt cgttttttaa atacacagaa
    19201 aatggatata cttttagaga attaaatgta gatgtaggta gataccaaat acagataatt
    19261 agaaatatag attctgagat gaattcttta agacagctta catctgttct tataattgga
    19321 atattaattt ctgtaataat tacttacttt gtggctgtat atctcactag aaaagcttta
    19381 attccaatag aaacagcatg gaaaaatcaa gctaagttta tacaagatgc ttctcatgaa
    19441 ttaagaacac caataacaat agtttcatca aaattagaaa gtatgttaaa atccccagaa
    19501 agtacagtaa atgatgaagt tgaaacaata gctactgcaa tgaaggaaac tagaagactt
    19561 aaaaaaatga taactgattt attgtctctt actaaagagg actctattgt taaagttaat
    19621 ttagaagaaa tagatttaga aaagttattg gaagaaatat cagaagacta tattgacatt
    19681 gctgagtttc aggaaaaaag attcgtgttt aattctaaac tgaagaataa agttataata
    19741 acagataaaa ataaattaag acaacttata ttaattttca ttgataatgc ctttaagtat
    19801 acaaaattgg gtgatgagat agcattagaa ttgaaagaag atatagagga tgaggtaact
    19861 ttacttataa gtgatactgg tataggcata aaaaaagaag agatacctct tatatttgat
    19921 agatttttta gaagtgaaaa tgtaagaaat aaagatttag aaggttctgg aataggactt
    19981 tcaatagctc gcatgattag tttaaactta tctattgata taaatgtaac ttctgatgta
    20041 gatattggaa ctacatttga attgtcaata ccaaaaaagt taaaataata aaaatataaa
    20101 aaattgtttt tagaaatgaa gaatgggtat actaattagt ataaaatata aaaagacctt
    20161 ataaaagtaa atttatattt ataaatttca tagtataatt atatagggat aataaagttt
    20221 aataggagga tttaaaaatg gcaaaagtta taaatacatc tgaatttaga agtagtgttg
    20281 aaggtagtaa gggagtagtt gtagttgatt tctttgcaac ttggtgtgga ccttgtaaca
    20341 tgttgggacc tgtatttgca gaattaggtg aagagatgaa ggataaagct agatttgtaa
    20401 aagttgatat agatgagagc ttagaaatag ctcaacaatt caatgtatct acagtaccaa
    20461 ctatgattat atttaaagat ggaaaaccag tagaaacttt aattggattc atgccaaaga
    20521 acaaaattga aatgcaagta aaatcatatt tataaaaaat gggtgagtat tatgagatat
    20581 gatatagcta taatcggcag tggtccagct ggattgtctg ctgctataaa tgcaaaaata
    20641 agaaataaaa caattatcat gtttggtaat gataatttaa gtaataagct tgttaaagct
    20701 ccttcaatag ataattattt aggtttttat gatataagtg gtgatgaatt aaaagacaag
    20761 tttaaatcac atatagatag tatggatatt tcaattgaaa ataaaagaat aaataatatt
    20821 tatgctatgg gagaatactt tacaatgatg tctggaaatg atatgtatga agctactact
    20881 gtaatccttg caactggggt agagtatact agaccaatca agggtgaaga agaatttcta
    20941 ggaagaggtg tgggctactg tgctacatgt gatgcaccac tttatagaaa taaaaaggta
    21001 gcagttatag gatataatga agaatctaaa gaagaagcaa attttttaag tgaactcact
    21061 tcaaagactt attttatacc tatgtacaaa aaagataatc ttatgagaag ttcagataac
    21121 ctagatgatt ctattgaagt tatacatgac agaccagtac agatagatgg agataaactt
    21181 gtaaacaaag tatcttttaa agaaaaccac attgaagttg atggagtatt cgttataaaa
    21241 gatagtacgg ctccaagtgc attggttcct ggaatagaga ttgatggcat tcatattaaa
    21301 gttgacaata atatgaagac tagtatagat ggatgctttg ctgctggaga ttgtgttgga
    21361 aaaccatatt cttatataaa agcagctggt caagggcaaa tagcagcttt aaatgctgtt
    21421 tactatttag ataaattaaa gagggcttaa tatttatatt gattaacttt ttaaagatat
    21481 ttataaaaaa ctgtcttgaa ataattttat tatttcaaga cagtttttta tttttaagtt
    21541 attaataaaa gtattttaaa atctatatgt aattactttt attaataaaa ctcaaagtta
    21601 tattagtaaa aattggaatt atttataata tactccaaaa tttaaaaata atggttgaaa
    21661 aatctaatta agagtactat aatttaatta agtaattact taatcaaaac gaggtactta
    21721 tggaaaactt ggttaaaata tttaaagcat tgagtgatga aactagactt aacatactta
    21781 tattagtatc caagagaaat atatgtcaaa aggggatttc taaatatcta gggatttcag
    21841 attctgctgt ttcacaacat ataaaaatat taaaagatgt tgggataata acaggatata
    21901 aagaaggata ctatgtttta tatcatatta ataaagaatc atttaatata tgtgtagatt
    21961 ttataaattc tatgttaagt aattctagtg aaagctttat agatgtattt gatgtaaata
    22021 caatacatct aggatgcagt aaagaatgta aatctataaa aaaatgttgt aagagaaggg
    22081 agaaataatg atgaaagtat gtataccagt agaggagaat aaaggattgg atagtaaacc
    22141 atatggacat tttggttctg ctccaatttt tgttgtgtgt gatttagaaa gtggagaagt
    22201 taaatcttta gataatggag atttagatca tgaacatgga aaatgtcaac cattaaaagc
    22261 attgtcaggg acagctgtag atgcagtagt tgttggagga ataggacaag gagctataat
    22321 taaattaaat ggaatgggaa taaaagttta cagagcagaa ggtgatacta taagtgctaa
    22381 tttagactta cttaaaaatg gaaaattagt agaatttcca tcagaccata cttgctcaca
    22441 tgacggatgt gggcatcatt aaaaaaaaca gctgatgaaa tatatacttc atcagctatt
    22501 ttttctttta ataaatacga ttttattctt ttaaaaccaa aatttagatt tttatgtttt
    22561 ttgtagtatc cttaataata tttcaagttt acaagtttac tacttttaga tttttttggt
    22621 atgtttttct atatagtcaa tatcatttaa tatgtcattg agttctttat taagtgtttt
    22681 taaatcatca ccatctacat tgtagtttaa aaatagttcc tctacattat caatgataga
    22741 attgaccttt ctagtaactt caacaaatgt cttaaagtta ctactatttt tagcatttag
    22801 aaattttttt aaatcctttg atataaatga tactatttca tattcatcat taaatagacc
    22861 tctaccaata ggatatggtt caattctctg taaaaaatat tggtctttag cattaatatt
    22921 ataagtataa gatatttccc aaccgtctcc taaatcaaac ttgataaaat ataatgtact
    22981 acttactttt gatattcttg tggcaccaag ctgtcttaaa agaacttcaa tcgaatcacc
    23041 ttgaacagta ttaatattca tatatacaca tcccctttag tattattata ctatattttt
    23101 taaaatttta taattaaata aacagtagat ataataatgg atataatcat aagtggaaaa
    23161 cctattttga aatactcctt aaaagagata ggatgaccgt gtttattacc tatacctgct
    23221 agtacaacat tagcagatgc tcctattagt gttccatttc ctccaagaca agctccaaga
    23281 gatgttgccc accatagtgg cattacatca ataccttcag cttgcatagt caaaataaga
    23341 ggaattagtg ttgcaacaaa tggaatatta tctaagaaag aagagactat agctgacaac
    23401 cataatatca acaacattgt aaatacaaga tgtccttctg taagtccaat caatgcttga
    23461 gccaatttgt ttattatccc aacttctact aatccaccaa ctactacaaa taatcccata
    23521 aagaataaaa ttgtagacca ttcaatacct gccataattt catctacatc ttgttttcct
    23581 attaaaagca ttatagatgc tcctgtcagt gctacaacag aagaatctat gtggattgta
    23641 ctgtgaaaca taaaaccaaa aagtatgaat gctattaata taaggctttt tataagtaga
    23701 ggtttatctt taacagattt tttttcatct agttttaata ttgcattttt agctctttca
    23761 ttcacaacaa gttcttttcc ataaataaat ctaaagcata ttatagttat agcaagtatt
    23821 actataacag caggacctaa atttattaca aaatccataa aactaagatt ggcagcacta
    23881 ccaatcataa tatttggagg gtctcctata agagtagctg ttcctcctat atttgaagct
    23941 aaaatctgtg ttattaaaaa tggaactgga ttaagtccta atatttgtgt aataactata
    24001 gtcattggtc caataagtaa gacggttgta acattatcta atacagctga taaaatagct
    24061 gttataatag caaagcaaat cattatcttc catgggtcac ctttggcttt ctttgcagtc
    24121 cagatagcta tatattcaaa taaccccgag tttttgacaa cagctacaaa tagcatcata
    24181 ccaactaata caccaatagt attgaaatct atatggctta atccttcatc aagtgtaagt
    24241 atattgaata ttatcattaa tagtgcacca ccaacagctg ctacggatct gtttaccttt
    24301 tctgtaataa tagctgccat aactattaaa aatatacata tagctattat ttgttgaaat
    24361 tccataatat aagtacctcc atttgtatta ttttgtgcct aatgctagta ctatatatag
    24421 tattactttt ttattgtttt ttcaatggat gtatgaaagt atattattta acaaagtttt
    24481 tgaaaaattg acagaattta aagaaaattt ttgttattta aataaatttt aaaattattc
    24541 cattgataat aatattaaac gacaaacgaa aaaatatacc ctttaaacta gaatttattg
    24601 tatccaataa caattataaa atattaaata tatctatatt ataaaaacaa taaaaaaagg
    24661 tttgatttga atataaaact gcaatatatt aaaaaaatta ttcattttaa accttgtaat
    24721 ttgattttat aaatattata attaaattaa tgaattttta acaaggaatt attcattttt
    24781 ttgattttaa gatttattga gaaaacaaaa ctaaataggg gggtaattaa tataatggat
    24841 tttttcaatg gtattttaac gagtattaac aatgcagtag tatatgtaaa caattactta
    24901 tggtcgtata tcttaattat aatgttagtt gtaattggta tttactttac tattaaaaca
    24961 aattttgtac aattcagata ttttatagaa atgtttagac ttctgggtga tggtacagcg
    25021 aataaaaatg caaaaaagga aggaaaaatc tcttcatttc aagcattttg tataagtaca
    25081 tcttcaagag ttggaactgg aaatatagct ggaatagcaa ttgcagtggt agcaggagga
    25141 cctggtgctg tattttggat gtggcttata gcattaattg gttctgcatc aagctttgtt
    25201 gagagtacat tagctcagat ttacaaagtg aaagatggtc aagcttttag aggtggacca
    25261 gcgtattata tggaacaagg tctaaataaa aaatggatgg gtattttatt ctcaatactt
    25321 ataactatat gttatggttt tgtatttaat gcagttcaag caaatactgt ttcacttgca
    25381 tttaataatg catttggaat aagtaaaatg gctatgggaa taggtcttgg aattttaact
    25441 gcaatagtta tttttggtgg agtgcataga gttgcaaaag tttctgaaat aatagttcct
    25501 gtatttgcag gattatatat attagttgct ttggctatag ttgtaatgaa tataacagag
    25561 ataccaagtg ttatagcatt gatagttgaa agtgcatttg attttaaagg aatggcgatt
    25621 ggtacgttta tgggagttgt aatgactgga gtgaagagag gtctattttc taatgaagca
    25681 ggtatgggta gtgctcctaa tgctgctgca acagcacatg taactcatcc tgtaaaacaa
    25741 ggtttaattc aatctttagg tgtttttaca gatactataa taatatgtag ttgtacagca
    25801 ttcatggtat tattatattc aggttatagt agtagtggag caactggaat tgaattagct
    25861 caagaagctt taactcatca tataggacca gttggaaata tatttatagc agtttgtata
    25921 ttcttgtttg catttagttc tatagttggt aattactatt atggtgaatc aaatatggag
    25981 tttatgagtg gaagtaagac taaacttaat gtatttagag tttttgttgt aggtatggtt
    26041 ttatttggtt cacttactca agtagatgta gtttggaatc ttgcagactt atttatggca
    26101 ttgatggcga taataaactt aatagcaata gcacttttag gaaaatatgc atttattgct
    26161 ttaaaagatt atacaagtca aaaaaaatca ggaataaaag accctgtgtt tattgcagat
    26221 gaaatagaag gcctagaaaa ggtatcagag tggcataggg aattatctga ggaacattta
    26281 ggataaaaaa tagtataaaa ttataaaacc attttgaaag actagagtat gtatatactt
    26341 tatatgaata taatattgaa ttaatttata ttactggaat ttttaattcc agtaatattt
    26401 ttttattata taatttagct ttattaaaag tttttattat taaaaatagc ttatttatcg
    26461 taaagtgaat gatattgggc ttttaattat tatgaatgtt agtatttaat ttaaagactt
    26521 aaataaattt gccatttcta gcgcattcat agcacaatca taacctttat ttccagcttt
    26581 tgtacctgct ctttcaatag cttgttcaat attttctgta gttacgattc caaatataac
    26641 aggtaattca ctgtctaaag atactttggc tatcccttta gagacttcac tacatacata
    26701 atcataatga gaagtagccc ctcttataac acagcctaaa cagattatag catcatactt
    26761 accactttta gccatttttt gagcaacaag aggaatctca aaagctccag gtacccaaac
    26821 tatttctata ttttctggag aaacgtcatg tcttaaaaga cagtcttcag ctcctgataa
    26881 aagttttgat gttataaatt cattaaaacg agaatttatt atacctattt ttaaatcttt
    26941 tccaataagt tttccttcat atatcatttt tatttcctcc aattttattt atgttataga
    27001 tttatattta taaatatctt taatttaatt ataaaaaact acttagtaga aaaatatcat
    27061 catataatca tttatataat atctaaaaga tgtcccattt tatctttttt agtttttaaa
    27121 tatagcttat taaagtcatt aatttcttca ttaataggga ttctattttc gatttttatt
    27181 ccatatttct ctaattgatt tattttatct gggttattac ttaaaagatt tatacttttt
    27241 atgtttaaat tttttaatat ttgagcagcc atatagaaat ctctcatatc ttcttcaaat
    27301 ccaagcatta aatttgcttc aacagtatcg taaccttctt cttgaagttt ataagcctta
    27361 attttattta aaagaccaat acctctacct tcttgcttca tataaattaa aactccactt
    27421 ccattttcca caattttctt catagaagat tctagctgta atccacaatc acattttaga
    27481 gagtggaaca catctcctgt taaacattca gaatgaagtc taactagagt attttctata
    27541 tttatatttc cataagtaag ggcaatgtgt tcttcatttg aataagtatc tctatatcct
    27601 atgatttcaa aatttccata ttttgtcgga aggaaagcac tagatacttt ttctatagta
    27661 gtttcattta ttttgcggta ttctataagg tcttttatag tgatgatttt taaattatgc
    27721 ttttctttga aaatcattaa atcatcaact cttgccattg taccatcatc tttcattatt
    27781 tcacagataa gaccaatctc tttaaaacct gcaagtctag caaggtctac tgaggcttct
    27841 gtatgtccat ccctgacaag aactccacca gtttttgcta ttaaaggaaa gatgtgacca
    27901 ggcgttttga aatctttact tgtagaattt tcatcacaga ataattttac agtttcagct
    27961 ctttcatatg cagatatacc agttagagta tctgctccat ctatagtaac agtaaaggct
    28021 gtttgaaatg tatctgtatt ttctctaacc ataagaggta tattaagact tttaaatttt
    28081 ttttcagtgg caggtagaca aattagacct ctaccatatg tagccataaa atttattgat
    28141 tcaggagtag ccatttcagc tgccatcaat aaatctcctt cattctctct actttcatca
    28201 tcaactacga ttaccatttt cccatttttt atatcttcaa tagcttcttc aatagtatta
    28261 aacatgtaat tcactccttt attattttta aaaaccattt ttaaataaaa aatctttatc
    28321 tatatttgat ttatggttac tactattaga atttaattcc ttgtagttat tagccataaa
    28381 attattaaca tactttccaa ttacatcgct ttctaaatta acgaaatctc caacattctt
    28441 tgtcaagaga atagtgttaa ttttagtatg aggtataata gaaaccttaa atatttcatc
    28501 atctaaatat gatatagtta aacttacacc atcaatagca acagaacctt tgagtatcat
    28561 tgaggataaa agttttttat caggtcgtat acttactaaa actgcatttt catctttttc
    28621 aaatttgcag atagttcctt taccatcaac atgacctgtt actatatgac caccaaatct
    28681 tgaagaaaga ctcatagctc tttcaagatt taccttgtca tttgcattta ataaacctaa
    28741 atttgtggaa cgaattgtct ccatcataac atcggctgta aattcatttt ttccgaggtt
    28801 tgaaacagtt agacaaactc catttacggc tatactatct ccaagatttg tgccatctaa
    28861 tattttattt gatagtatgg ttacttttcc actcttacca ttagcagtaa tttttctaag
    28921 tattccaact tcttctacta tgccagtaaa catcctaacc tcctatatga atgtattaac
    28981 aaaaatttaa tttaaaacat ctgattctat aagaatatcg ccatctatca aagttacatt
    29041 attgattgtt aactgtgttg aatctgacaa atgatttatt ccttctccag aaacgaatgt
    29101 aggtgcatct ttaccaccta taatcttggg tgatagaaat aatttgactt tatcaactaa
    29161 cttatttcta aagagactgt cattgagagt agcaccacct tcaactagaa cagaatcaat
    29221 gtttaactct cctaatttat acattacaaa ctccaaatct actaagttgt tttttgaagg
    29281 agaaattatt gtttcaacat tattttcttt taacatagat aattttatac tttctttgca
    29341 acatgtgaaa actatagttt tactggtttt atctttaacc aaattactat taagtggcaa
    29401 ctttaggtgt gtatctatta ctattcttgt aggatgagac actttttctt gattgagcct
    29461 acaagttagt aaaggattat cgttcaaaac tgtatttatg ccaaccatta tactttggta
    29521 tttatttctg tatttatgag ttagatacct tgatgattca ttagatatcc attttgattc
    29581 tagtgatttt gtggcaattt taccatctag acttactgct gattttacaa tacaaagagg
    29641 tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa tctcttgctt catcttctaa
    29701 tattcccaca gtaacatcaa tattgttatc ttttaatttt tttactccat taccacaaac
    29761 aagtggattt gggtctaatg tagatattac aactctctta atcttatttt gtataatttt
    29821 atctacacaa ggaggcgttt tcccataatg agaacatggt tctaagttta cataaagagt
    29881 agagtctttt agactttgtt tagcactgtt tatagcattt acttcagcat gattagagcc
    29941 aaatttctca tgatatcctt tgcctattat attactatct ttaacaatta cacacccaac
    30001 taatggattt ggatttacaa agccttctcc atttttagcc aattcaatag cttttttcat
    30061 ataataaata tctttttctt tttgattcac aaaattcaca atatgtacac ctcctgttta
    30121 attggataat ttttaaacaa aaaaccctga gaattaactc agggtttaga ataatctaca
    30181 ataaatgttt aataaatgta gaacaaaatt gctctacaaa aacactcatt aaatatcttc
    30241 ttccatccag actttactgt cggctttgga atttaaccaa atcaaccaag ttttacattg
    30301 gctcgcgggc tataccgccg attgggaatt tcaccctacc ccgaagatta agtattaaat
    30361 ttatatttag aatgtatcat aaattatttt actttgcaaa ctattttttt aaaaaatata
    30421 tgttaatata taatatagaa tgtctataaa ctttgaaata ccacgattgt gaatataaaa
    30481 ataaaaattt gaacataatc aggaaagaag tctaattaat ttaaattagg agagtaatat
    30541 atgaataaaa aacttttaga aatactatca aaatactatg gatatacaag ttttagaaaa
    30601 ggtcaggaat ctataataaa ttctatctta agcgaaaaag atgtgctagc tattatgcca
    30661 acaggagggg gaaaatcaat atgttatcaa attccagctt tgattttaga tggaatgaca
    30721 atagtaattt ctccacttat atcacttatg aaagaccaag tagatgcctt aaagactatg
    30781 ggaattagtg ctgcttttat aaatagttca ttaagcagca aagaatttaa tgaaatatta
    30841 aataatatta gaaaaaataa ttataaaata ctctatattg ctccagagag attagatgca
    30901 caagagttta ttgagctaat aaataataac aatgtaagtc aagttgcgat agatgaagca
    30961 cattgtgtat ctcaatgggg gcatgatttt aggttgagtt atagaaggat ttctgatttt
    31021 ataaaaaatt tgcctaaaag acctatagtt acagcattta ctgcaacagc ttcagaagag
    31081 gtaagaactg atataataaa tttactgtgt ttagaaaatc cagattatta tataactgga
    31141 tttgatagag agaatttatc aataaatata gtaaaatcat ctagtaaaaa taagtatatt
    31201 ttagattata ttcaaaatca taaaaatgag tctggaatta tatatgttgc aactagaaag
    31261 gaagttgaaa atatatataa tggtttatct aagagaaata tatctgtatc aaaatatcat
    31321 gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag attttataaa tgataatgtg
    31381 gatataatgg tagcaacaaa tgcatttgga atgggtattg ataaacctaa tataagatgg
    31441 gttatacatt acaatatgcc tcaaagtata gaaaattact atcaagaaat aggtagagct
    31501 ggtagagatg gagaaaaaag tgagtgtgta cttttatttt ctccaggaga tgtacatata
    31561 caaaaatatt taattgatat tggagttgaa aatcctgaaa gaaaattatt tcagcataag
    31621 aagcttcaat acatgattga cttagtttac agtaattcat gctatagaaa gacaatattg
    31681 agttattttg gagaaaatta tatagacaat tgcaataatt gtagcaattg tttagtagag
    31741 ggtgaaatag tagataaaac tatagatgca caaaaagtca tttcatgtgt agctagaatg
    31801 aaaagaagtt atggtgttac tactattgta gatgtgctta gaggttctaa gaataaaaag
    31861 attttacagc ttggattaaa tactttgtct acatataaca ttatgagaga ttattctagt
    31921 gaagacttaa agaattttat aaatacattg gtttctcatg gttttttaga tttagttgag
    31981 actctaggca atggaaatag aggtagtttt cctactataa gattaaatga gatgtctatg
    32041 aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa ttgttatgac aaaatcattg
    32101 gaagtagagg atgagttata taatgcttta agagaactta gacattcaat tgcaagtgaa
    32161 gaaaggatag ctccatatat ggtatttgga gatggtacac ttagagccat gtctagtagt
    32221 tatccaatta ataaagaaga aatgctagat atctctggag taggagaaat taaatatcaa
    32281 aaatatggta gagattttga agcagcaatt aaagaatatg tcgaaaaaaa taatataaat
    32341 aaaaaaatag atgaaaaaaa tgtcgattca aagaacgtta atagtgattt cttagaggtt
    32401 aatacagata aagttttata tgaaaaactg ttagtcatta gagaagaata tgcaacaaag
    32461 gaaaaattac ctgcatatat gattttagcc aataaggctc tgaaagaaat aagtggaaga
    32521 tatccacttg atgaagaaca attaaaagat atttctggca taggtggagt aaaatttgaa
    32581 aaatatggag ctagtatttt ggcagaggtt aataaatatg taaaagaaaa taatattaat
    32641 gttatttggg agaaaaaggg caaaaggaag ttaatattag atggagaaag tagaaaaaat
    32701 aatgaaatag cattagattt gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa
    32761 atagaagttt cactgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat
    32821 agtatatcat ttgaattagg actagaaaat ttttataatg aagataaaaa gaagatgatt
    32881 ttagaagttt gtgaaaaagt tggctatgat aatttaaata acataaaaag aaaacttcca
    32941 gattcaataa agtatgaaaa tataagagct gtgatacttg actcttatct tgaaaactta
    33001 aaaaataata ttatagcaga gtaattttta acttaataaa aaataaaatt tgtattattg
    33061 tgttgaacta ataattttgc tgtgttatac tgaatttgta aaaataatga aaaattagag
    33121 ctaggggcgc tagaattggc tagctgagag ataaagccgt aacttacaaa ccctaataac
    33181 ctgatcaaga taatgcttgc gaagggaagc tttttaatat aattttatta tataaatagg
    33241 caaattattc aatgttagga gaaatttgtc taagtgtata tacgattata agagtgcttt
    33301 ccaaacgagg aaaagcactt tttttatttt caaaaactat aactttaatc ttggaggatt
    33361 gtaatatgaa ctatacaaca caaatggatg ctgctagaaa aggcataata actaaagaaa
    33421 tggaaatcgt ttctcaaaaa gaacaggtag atgtaaatga attaagagaa ttaatagcaa
    33481 atggacaagt tgttatacca gctaataaaa atcataaatc tttaagtgca gaaggtgtag
    33541 gtaaaaattt aagaacaaaa ataaatgtaa atctaggaat ttcaagagat tgtaaggata
    33601 tagaaaaaga acttgaaaaa gttagagtag caatagacat gaaagctgaa gctataatgg
    33661 atttaagtaa ttatggcaaa actagagaat ttagagaaaa ggttgttgaa atgtcacctg
    33721 caatgatagg ttcagtacct atgtatgatg cagtaggata tttggaaaaa gaattaaaag
    33781 atataacaga agaagagttt ttaaatgtaa taagacaaca tgctattgat ggagtagact
    33841 ttattactat acatgcaggt cttacaagaa gtgtttgcca aaaaataaaa aatcatgaga
    33901 gattgactca tatagtatct cgtggaggtt ctttattgtt tgcatggatg gaattaaata
    33961 ataaggaaaa tcctatttat actaattttg ataaaatatt ggatatatgt gaagaatatg
    34021 atgttacatt gagtttagga gatgcttgta gaccaggttg tataaaagat tctactgatg
    34081 gtgttcaaat tcaagaactt gtagtattag gtgaacttac taaaagagca tgggaaagaa
    34141 atgtacaagt aatgatagaa ggtcctggac acatggctat tgatgaaata gaagcaaatg
    34201 tagtattaga aaagagatta tgccatggag caccttttta tgttcttgga ccattggtta
    34261 cagatatagc accaggttat gaccatataa caagtgcaat aggaggagct ttagcatgtg
    34321 ctaaaggagt agatttttta tgttatgtaa ctccagcaga acatttaaga ttgcctaatt
    34381 tagatgatat gaaagagggt ataatagctg caaagatagc tgctcatgca ggagatatag
    34441 ctaaaaatgt taaaggtgct cgtgaatggg ataataagat gagtaaagct agagctgatt
    34501 tagactggtg tgagatgttt agacttgcaa tagaccctga aaaagctaag agatacagag
    34561 atgaatcaac tcctactcat gaggatagtt gtactatgtg tggaaaaatg tgctcaatga
    34621 gaacagttaa gaaaatatta aacaatgagg aacttaatct aatataattt ttggctagta
    34681 tgactggaat aaaaagaagc tgaaaagagg aggtgattta gtgaaggtta atgggaagga
    34741 aattgaattt gaaaaagatt tgactgttat agatttatta aataaatata atttaaaaag
    34801 tgatagagtt gtagttgaag tgaatttaga gataattgaa gaatctaact acaatacata
    34861 tgtattaaaa gacgaggaca ttgtagagct tattagcttt attggaggtg gttaaaataa
    34921 aaatatttgt aaatgaatta ttaacagatg ttgaggaaaa tataactgct tataaagtta
    34981 gagatagcta taacaataaa tgtgatgtat tggtgttaaa tggatatcca attaaaacag
    35041 atatgcccct ttgtgaaaat gataaattaa cacttataca aaaaggagtt aagccatcac
    35101 ttgatgaatt ggaaagcttg ttgattgcta gacatactcc aaatatacat aataaattaa
    35161 aaaaagggaa agttgctata ttgggattgg ggggacttgg ttccaatata gctatctctt
    35221 tagctagaat aggagtggga gaacttttac ttatagatta tgatgttgta gaaccatcta
    35281 atttaaatag acagcagtat tttatagatg atataggaaa actaaaaact gatgccatga
    35341 tagaaaatat aaaaaaaata aatccattta taaaattaaa taaacgagat atttttttaa
    35401 acaagaacaa tatggatact attaaggatt cagatttaat aattgaggcc tttgatgatg
    35461 cttcatgtaa agcacaggta tgtaattatg tattaataaa tttaaaagat aaatatttaa
    35521 tagcatcatc tggtatggca ggttactatg attcaaatat aataactact aagaagataa
    35581 aagacaggtt ttatatatgt ggggattttg tgaatgaagc taaatttggt gaagggctta
    35641 tggctccaag ggttgccatc tgcgcaaatc atatggcaaa tttggcaacg caaatactta
    35701 tagatatgta aatattaaaa ttagatataa aaaagattga atattaaaaa aatttaacgt
    35761 ggagggaaat aatatggata aattgatact tggtggacat gaatttaata gcagattatt
    35821 ggttggaaca ggtaaatatg gttcaaataa tatattgcct gaggtaataa aagaaagtgg
    35881 aagtgaaata ataacaatgg cacttagaag agtggattta gacaataaac aagaaaacat
    35941 attaacgtat attccaaaag agatgacaat acttccaaat acatcaggag ctactaatgc
    36001 agaagaagct gtaagaatag ctagaatatc tagaaaaatg ggatgtggag attttataaa
    36061 aatagaagta atatcagata ctagatattt acttcctgat aatgaagaaa ctataaaagc
    36121 tacaaagata cttgctgatg aggggtttat agttcttcca tatatgacac cagaccttta
    36181 tgctggaaga agacttatag aagctaatgc agcggcagtt atgccacttg gagctccaat
    36241 tggttcaaat agaggtcttc aaatgaaaga aatgataaga ataatgatag atgagttgga
    36301 tataccaata atagtagatg caggtattgg aaaaccatct caagctatgg aggctatgga
    36361 aatgggagct gatgcagtac ttgtcaacac tgctatagct tcagcaggag accctgtaca
    36421 aatggctaga gcatttaaat tagctgtaga aggtggaaga gaagcatata ttgcaaaaac
    36481 aggaaatgtg tcagaatttg caaatgcatc ttctccttta acaggttttt taggcaattt
    36541 atagaaagtg gtgattggtg atgagttttt atgatgtaat agaaaaatat agggattttg
    36601 attttgatgg atatttaaat aatgttactg ataatgatgt tttaagaagt ttatcaaaag
    36661 acaagcttga agattttgat atattaaatt tgctttctaa aactgcagta aaacatttag
    36721 aagatatggc acaaaaagca cataaattaa gtgttcaata ctttggtaaa acagtatgtt
    36781 tatatacacc aatgtatata gcaaattatt gtgttaatca atgtgtttat tgtagctata
    36841 atataaagag tggaataaaa agaaaaaaat taactatgga tgaaattaga gaagagggag
    36901 aagcaatttc aaaagaaggt ttcaaacatc tacttgtttt gactggagaa agtagttttc
    36961 actcaagtgt agaatatata ggtgaggcta tagaaatatt aagagagaaa tttccctcta
    37021 taggtataga agtttatcca atggaagtag aagaatataa gtacattgta gataaaggtg
    37081 ttgaaggatt aacagtatat caagaaactt atgacgagga aatatataag agagtacata
    37141 taaaaggccc aaaatcaaat tataaattta gactagatgc tcctgaaaga ggggcaaaag
    37201 caggtatgag gactttatca ataggtgcac ttttagggct aaatgatttt agaaaagaaa
    37261 ctttttttac aatattgcat gggaaatact taaaaacgaa atatcctcat attgagcttt
    37321 catattcaac accaagaatg agacctttta aagggtgttt tgaggaattg gtagatataa
    37381 gtgatactga tttagttcaa gctatggtat gtatgagatt atttgaccct catgcagcta
    37441 taaacatatc cactagagaa aatttagaaa tgagaagtca tataatacct ttaggagtaa
    37501 ctaaattgag tgcaggtgta tctactgatg taggaggtca ttctcaagat gaacatgata
    37561 ctgctcaatt taagataaat gatgaaagta ctgttaaaga tgtggagaaa atgctaaact
    37621 caattggtta tcagcatgtg tttaaagatt gggaaagatt ttaaatgtat ctgataacaa
    37681 acagaaaatt atgtagtgag gaacgatatt tagaagttat taaagagtct atattgagtg
    37741 gtgtagaaaa tataataata agagaaaagg atttagaata tcaagagcta agaaaattat
    37801 atatgaaaat aaaaactaaa atcaattgta tagattttca agagcaaata tcagatgaaa
    37861 gtttgaaaac taatataaat caaaaagagt ttagaaataa attcaaggtt aattttataa
    37921 taaatagtaa cattgaattt tttgaaaaag tggattgtca aggtatccat cttcctttta
    37981 aattattctt gaatttaatt gaaaataagt ataattttaa tgaaaataaa atactaggat
    38041 tatctttaca taaagtagag gaagtagatt atttagaaaa attaataaga aatcaaaata
    38101 taaaaataga ttacataaca ttatcacata tatatgaaac taaatgtaaa gaaggattaa
    38161 atccaaaagg aattgagctt ttaaaagaag ctaaaaagat tactgatatt aaaataattg
    38221 cacttggggg aattttacca tctaatgtca aggaaacatt aaaatactgt gatgattttg
    38281 ctataatgtc aactataatg aggtctaaag atattaaaaa aaccatatca aattataatg
    38341 aaaagttgaa ttagtaaata tagtatattg aatttttaac ttaaataaaa ataaaaaagt
    38401 tatcttttaa attttaaaag gtagcttttt tattttatat ctttgctaat aaaaaattgt
    38461 aaaaagggaa aactttagtc agagagatta aacaatatcc tagaaagatt taaaatttag
    38521 taaagtatgg aggacattat aatgactgac agtaaaaaga aacgatttat tataccacac
    38581 acttttacaa taatattttt actaatagtc ttgatggcaa tattgacttg gatagtacca
    38641 agtggtgaat ttagtagaga agatgtagaa ggtagaatgg tagttgtacc agggacttat
    38701 caaagtgtag aaagtaatcc tcaaggattt gcagatgttt ttaaagcacc aatagaaggc
    38761 tttatcgatt cagctgaagt tgtaggtttt gttttaatag ttggaggtgc ttttggtatt
    38821 gtaaatagga ctggagctat agaagcagga atagcttcgg ttatctctaa gtttaaggga
    38881 aaagaacttc ttataattcc aatatcaatg attttatttg gacttggagg tacaacattt
    38941 gggatggcag aagagacatt accattttat atgatatttg ttccactcat gacatccttg
    39001 ggatatgatt ctctaacagc aatagcaatt gtatttatag gagcagcagc aggagcagcc
    39061 gcttcaacaa taaatccatt ttcagttggt attgcacagg cactagctca acttgctcct
    39121 ggttcaggag taacttacag agtagttatt tttatagcat atatactaat aagtatagca
    39181 tttgttatgt actatgctaa gaaggtaaag gaaaatcctg aaagttcaat agtatatgaa
    39241 atagataaaa ataataaatc aatacttaat caaacttcaa cagaaataaa agaatttact
    39301 tttaaagaag tatctgttct tgctatattt ggaataggga tggcaataat gatttggggt
    39361 gttttaagtc agggatggta cattccagaa atatcaatgt gttttgctgc aataggtgtg
    39421 ctttctggat tggttggtag attatctcaa attgaaatct cagaaagttt tatagatgga
    39481 gcaaaagatt tagcatctgc agctcttgct atagctttgg ctcgtggaat cgtaataatt
    39541 gctcaaaatg gatttataat agatactata ttaaattccg cagcagcctt tctaggtggg
    39601 cttccaaagg ttatatttgt atatttatca tttttcttgg aagctgcatt ggcattttta
    39661 attccatcat cttcaggtct tgcttcactt acagtacctg tacttgctcc tcttggtgat
    39721 ttggttaata tatcggctca agtgatagta accgcttatc aatttggtgt gggtcttaca
    39781 aactttataa ctccaacttc aggagtactg atgggagcac ttgcagtagc ccatattcca
    39841 ttttcaaaat gggtaagatt tataatgcct atcttgttga ttttgacagc tgtatctcta
    39901 gtctttttaa caataggtat ttatataggg ttttaattaa gtgtagtttt atttaagata
    39961 tttagtaatg gtataaaatg ataatatgtt ttcaataaat tgccactaat ttaattagtg
    40021 gtaatttgtt ttttgtattt aaaaataaat gtatttactt gtattttgaa ttaatatcta
    40081 catagacatt gttttaattg ttatattttc atttaattaa gcattgattt atcatttttt
    40141 atatttcata taacaatgca aaaaataatt tttatatagt aaatgacaaa agtttacaat
    40201 aaatgtatat tttcttactc attccttaaa attactcatt tagtttaata actctcattt
    40261 catatgtaaa gttggtaaaa aaattaaaat taaaagtaaa aaactaaata aattaatgtt
    40321 aatttgaaat atatatgaag gtttttggag aatacttact tatgatagga aaaaatgtta
    40381 aaataatagt gtaatcaaat ataaaggact gaaataatta acagttatta aatattagga
    40441 gtgtaaaaat ttatgggatt aaaagaattt gattttattg aagagtctat tgatatgttg
    40501 aggacaatgt caccaacatt agaaactata tcagatgaaa tagaagagta ttttgaaaat
    40561 atcttggatg aaaagaatca agaatatata aatgtaacct ctagaataaa atcagaatca
    40621 agtttaagag aaaagataat tagaaataga tatttaaaaa aatatggaga agcaagtaat
    40681 cttattcata atgtttctga cttaataggc ctaagaattg agtgtagatt tattgaggat
    40741 gaaaacaaaa tatatagact tttgagaaga tattttaata aaacagatga caaaataaac
    40801 tattataata aagagaataa gaatattaaa ttaaaactat ctgagaggca acccaataaa
    40861 caaaaaaatg gatttgaaat atataaaata gatggagttt ttgcgtattt agatagagaa
    40921 gtaaaatttg aacttcaaat taaatctctt gtaaatgtgt tttggagtga aatagaacat
    40981 aaaataattt ataaaaacaa tacatattta cttgctgata aatttataaa ggatatgatg
    41041 gactctataa agaataatct tactatgata gataatcagc ttttaagtat ttataaaaac
    41101 tttcattcag ggaagtcttt taatatgaaa gtaagtaaaa aagaaattga aaaattattt
    41161 gcgaaattag tctatgacgc tttttctgaa aagatgaata aaagtatagg ttttgttgta
    41221 gattttaaaa aaccatgtga gactatattg aactattctt ttaataaaca agatataagt
    41281 gatgaagttt taggtaattt tatgcttgat gagtttgcaa gattaaatga aattgtaaac
    41341 aaggatatag attttaatga gcaaatagaa tttgaaagag aaccaacatt tgatgataag
    41401 ttttgtaaag acctaggaaa tcattttaga agtagattaa acacagaatt tccatggaac
    41461 ctatttttta gaatattatt tgaaatagag ccatataaca ataccaaaga ctttgaaaac
    41521 tttgtagagt ttataaaaga aaatgtgcta ctagaagaaa atagggagca attattatgc
    41581 caatttgaag aagatagcaa acttataatt gaagatatat atgattgtat atttaatagt
    41641 ataagtgaga ttgatagtgt tgaaatttta tataactata atcttgaaaa aataaacttt
    41701 acatcaagtg aaatcataaa ttgtatatgt agagaatatg agtgttatgg tgaatactta
    41761 gaagaaaaag aaaagcttat gaatacattt aaagaaaaga ttattcaaat ctttgaataa
    41821 taggttaatg ctgaagtggc gctaatgcgc catttttcaa atatatatga attaagtaaa
    41881 attattttgg aggaggcgat ttgtatagag aaaacaggtg taattttggc agggggaaga
    41941 aattctagaa tgggtagaga taaagccttt ttagagttac atgataagct ttttattgaa
    42001 attgcaatag aagcatttaa aaactttgat gagttgataa taatatctaa taatgaagaa
    42061 ttatattcta aatacgatat taaagtttat aatgatatag taaaagatgt tggtccaatt
    42121 ggaggaatat atactgctct tgaatatgca aagtatgata tagttacaat tgcatgtgat
    42181 atgccatatc taaataatca aatagtcgaa agaatagcta ataaaatgaa tgataaaagt
    42241 gtgatttcag tgacaaatgg aaagttacaa ccattgtgtt ctggatataa aaagagtata
    42301 ataaataaag tatctttatg tataagggaa aatgatttaa agctcagaag ctttatagat
    42361 aaaatagata aaagttatat atactttgat gaagaagatt tatttttaaa tgtaaacact
    42421 gttgatgagt atgaaaaatt aactaaagtt taattagcta tggttgtaaa atagagtagg
    42481 atttatatgg aggagagttt atgaatttac ttaaatgcga ttcttgggca gtaatattaa
    42541 ataatagcga taaagaaagt aaagcatata aaatattaga tgaacttaaa aggaatatgt
    42601 acaaagttgt tgctatagat gaagagaaaa aacctattga aggaatagat gtgtatgaat
    42661 gtttaaaaga tgttccacac aatattgatg ttgtagctat tattgataaa caatctaaga
    42721 tggatgtgat tttagaagaa gtagagcttt tggatataca aaatatgtgg tttgaaaaag
    42781 gaagctttag tgaaatgatg attaaaaaga ctaaagattt aaaattaaat atagaatata
    42841 atttaagttt atatgatgag ttgactagat a
//
LOCUS       DAENCV010000024        42842 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAENCV010000024 DAENCV010000000
VERSION     DAENCV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.024"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(88..1095)
                     /locus_tag="KQD82_001212"
     CDS             complement(88..1095)
                     /locus_tag="KQD82_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG4379690.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1323..2207
                     /locus_tag="KQD82_001213"
     CDS             1323..2207
                     /locus_tag="KQD82_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4379691.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2409..3959
                     /locus_tag="KQD82_001214"
     CDS             2409..3959
                     /locus_tag="KQD82_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG4379692.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETEDLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3988..4635
                     /locus_tag="KQD82_001215"
     CDS             3988..4635
                     /locus_tag="KQD82_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG4379693.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(4777..5037)
                     /locus_tag="KQD82_001216"
     CDS             complement(4777..5037)
                     /locus_tag="KQD82_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG4379694.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5254..6537
                     /gene="hflX"
                     /locus_tag="KQD82_001217"
     CDS             5254..6537
                     /gene="hflX"
                     /locus_tag="KQD82_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG4379695.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6545..7222
                     /locus_tag="KQD82_001218"
     CDS             6545..7222
                     /locus_tag="KQD82_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG4379696.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7308..7751
                     /locus_tag="KQD82_001219"
     CDS             7308..7751
                     /locus_tag="KQD82_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG4379697.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8056..8700
                     /locus_tag="KQD82_001220"
     CDS             8056..8700
                     /locus_tag="KQD82_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG4379698.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8786..9163)
                     /locus_tag="KQD82_001221"
     CDS             complement(8786..9163)
                     /locus_tag="KQD82_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG4379699.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9163..9549)
                     /locus_tag="KQD82_001222"
     CDS             complement(9163..9549)
                     /locus_tag="KQD82_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG4379700.1"
                     /translation="MKKTDDLKQKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9728..10483
                     /gene="nadE"
                     /locus_tag="KQD82_001223"
     CDS             9728..10483
                     /gene="nadE"
                     /locus_tag="KQD82_001223"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG4379701.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10566..11285
                     /locus_tag="KQD82_001224"
     CDS             10566..11285
                     /locus_tag="KQD82_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG4379702.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11452..12456)
                     /locus_tag="KQD82_001225"
     CDS             complement(11452..12456)
                     /locus_tag="KQD82_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG4379703.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12723..13619
                     /locus_tag="KQD82_001226"
     CDS             12723..13619
                     /locus_tag="KQD82_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG4379704.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHINIKSDSCNC"
     gene            13757..15019
                     /locus_tag="KQD82_001227"
     CDS             13757..15019
                     /locus_tag="KQD82_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379705.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15095..16288
                     /locus_tag="KQD82_001228"
     CDS             15095..16288
                     /locus_tag="KQD82_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG4379706.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16917..17711
                     /locus_tag="KQD82_001229"
     CDS             16917..17711
                     /locus_tag="KQD82_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG4379707.1"
                     /translation="MESVMENLNNLKVELKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17806..19146
                     /locus_tag="KQD82_001230"
     CDS             17806..19146
                     /locus_tag="KQD82_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG4379708.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19283..20515
                     /locus_tag="KQD82_001231"
     CDS             19283..20515
                     /locus_tag="KQD82_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG4379709.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20555..21697
                     /locus_tag="KQD82_001232"
     CDS             20555..21697
                     /locus_tag="KQD82_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG4379710.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21708..22502
                     /locus_tag="KQD82_001233"
     CDS             21708..22502
                     /locus_tag="KQD82_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG4379711.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22513..23535
                     /locus_tag="KQD82_001234"
     CDS             22513..23535
                     /locus_tag="KQD82_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG4379712.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23634..25010
                     /locus_tag="KQD82_001235"
     CDS             23634..25010
                     /locus_tag="KQD82_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG4379713.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25342..25686)
                     /locus_tag="KQD82_001236"
     CDS             complement(25342..25686)
                     /locus_tag="KQD82_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379714.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            26174..27184
                     /locus_tag="KQD82_001237"
     CDS             26174..27184
                     /locus_tag="KQD82_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG4379715.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            27320..27808
                     /locus_tag="KQD82_001238"
     CDS             27320..27808
                     /locus_tag="KQD82_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4379716.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            27984..29912
                     /locus_tag="KQD82_001239"
     CDS             27984..29912
                     /locus_tag="KQD82_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4379717.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALEEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30089..30499
                     /locus_tag="KQD82_001240"
     CDS             30089..30499
                     /locus_tag="KQD82_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG4379718.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            30521..30823
                     /locus_tag="KQD82_001241"
     CDS             30521..30823
                     /locus_tag="KQD82_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG4379719.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(31040..31732)
                     /locus_tag="KQD82_001242"
     CDS             complement(31040..31732)
                     /locus_tag="KQD82_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG4379720.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(31758..32945)
                     /locus_tag="KQD82_001243"
     CDS             complement(31758..32945)
                     /locus_tag="KQD82_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4379721.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            33281..35158
                     /locus_tag="KQD82_001244"
     CDS             33281..35158
                     /locus_tag="KQD82_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4379722.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQENQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEIIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            35468..36874
                     /locus_tag="KQD82_001245"
     CDS             35468..36874
                     /locus_tag="KQD82_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG4379723.1"
                     /translation="MNTGFPKDFLLGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHSFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            36902..37750
                     /locus_tag="KQD82_001246"
     CDS             36902..37750
                     /locus_tag="KQD82_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4379724.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            38411..39103
                     /locus_tag="KQD82_001247"
     CDS             38411..39103
                     /locus_tag="KQD82_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379725.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            39100..40035
                     /locus_tag="KQD82_001248"
     CDS             39100..40035
                     /locus_tag="KQD82_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379726.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            40183..41106
                     /locus_tag="KQD82_001249"
     CDS             40183..41106
                     /locus_tag="KQD82_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379727.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            41090..41860
                     /locus_tag="KQD82_001250"
     CDS             41090..41860
                     /locus_tag="KQD82_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379728.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            41870..42652
                     /locus_tag="KQD82_001251"
     CDS             41870..42652
                     /locus_tag="KQD82_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379729.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT        15957 a         5080 c         7908 g        13897 t
ORIGIN      
        1 tggtctgtag gttaaataaa agtaccttta gaccaaatat attattaata acttaataac
       61 atcttaaaac ttatttattt atattattta caacatccat ttaataaata caccataacc
      121 tacagctggg tcgttttcaa ttgcatgtga cactgctccg attattttat tatcttgaac
      181 tattggagta ccactcatac cttgtacaat accaccagtt tgagctaaaa gctttttatc
      241 tgtaatttta attttaaaac ttttactttc tggatgtttt tggttttcta tatctataat
      301 ttcaatatta tattttttac acttattaaa gttatcttgt aaaattattt gagcttttcc
      361 taatttaact tcatctaaag atgctacttt atattttttt aacttggaaa tatcaaagtt
      421 attaatttga cctcttattc caaaatcagt attttctgta aagctaccaa taatattttc
      481 tcgttttgca tttatacatc caacactacc tttataagat ttttctatat taaggtgctt
      541 agttgtagaa attgaacctg ttttaatagg tatttttctt gcattaccaa cacttattgg
      601 atgacctaca gctccaaact ctgatgtatc tggattgata tacgttaaag tagcaaatcc
      661 agaaagtaaa ttgttaaaac taattttttc taatatgtgt ttggtagttt tcattgttat
      721 aagttgtcca cctctattta ataaaataga tacttgtttt tcttctggta aagcatttaa
      781 ggtattggca aagtcactat atccatcaac ttcagcatta tttaccttta ctatagcatc
      841 acctaatcta aatggagatg cttctccagg atttctaact atgatatttt ttaattctgc
      901 gtcaatttga agtacgtttc caattggtat taaatattca ggttcatttt tttgtaaatt
      961 ttgtgcataa attaaattat ttgagaaaaa atataataaa aataaaaatg ttaaaacaat
     1021 tgtaaatttt gacttagcta agttggtttt tttacttaaa gtattatttt taaaatgaaa
     1081 atttttaagt tgcataaaac acctctttct tattaaatta ttttaataat agaattgaag
     1141 ttttcttgct gcttctatat tatgttgtgc aagaatcatg ttttgatgct tggaaaacta
     1201 gctcaataac accttagttt tcataaaaat aaataaaata aatagtgtat atgataaaat
     1261 aaatacatat tcatataaat aaaaataaac aataactgtc tagcataaga aaggagcaaa
     1321 aaatgaaaaa atatagattg ttagttactt gtataattgc catgggaatc ataattatag
     1381 gaacatcaaa tccagttagc aatttttaca agactacaaa tttgattagt tcaggaaata
     1441 tcacaaatga aaaagcagat aaagatgaaa attcaaacaa aaataataaa gataaaaatg
     1501 aagacaaatc taattctaaa agtaataaag ataagtcatc agaggacaaa tcgtcagata
     1561 ataaaaatga aattcaaaaa aacaaaaaac ttttaatttg tatagaccca gggcatcaag
     1621 gaaaaggaga tagcaattta gaaccagtag ctccaggttc ttcctctaaa aaagcaagag
     1681 tatcttcagg aacagagggt attgctacaa aaaagccaga atatgtttta aacttagaag
     1741 catctcttgt tttaaagagt atattagaat ctaaaggtta taatgtaata atgactagag
     1801 agacacatga tgtaaatata agtaattcag aaagagcaat acttgctaat gacaaaaaag
     1861 cagatatggt tgtaagaata catgcagata gtttaaataa ttcttcaaaa actggagcat
     1921 ctatattgat tcctgaaaaa gatggaaaat atacagctcc tatatatgaa gaaagtaata
     1981 aatgtgctga atttatcaaa cagaatatgg aacaatcagg tatacaaatc aatggaatcg
     2041 ttcaaagagg tgatttgact ggattcaact ggtcaaaagt accagctgta ttagtagaaa
     2101 tgggatttat gagtaactat aatgaagacc aaatgatgtc aaatccagac taccaaagaa
     2161 aaatgatgca gtgtatagct gatggattgg atgcgtattt taaataagat atgaatagag
     2221 aaaaagttag gttggtatta ttttcctaat ttttatctct attttttatt gtacataaca
     2281 caaagaagaa gtttatttta ggttaaattg tcgtaaatat aactaaaaat attgggaata
     2341 cttgatttaa gtttaaaaag aatatattat agtatagaat gtgtaaaatt tttttgagag
     2401 gtgttgaaat gctagtagta gaaaatgtga gtcatggatt tggtgcgaga acaatattag
     2461 aaaatgtatc ctttagactt agaaaaggtg aacatattgc actagttggt gctaatggag
     2521 aaggaaaatc gagtttttta aatataataa caaagaagct tatgccagat gctggaaata
     2581 taaaatggtc ttcaagagct acagtaggtt acttagacca acatacggta ttaagcaagg
     2641 gaaaaactat aagagaagtg cttagagagg cttttaagca tatgtttgac ttagagcaag
     2701 aaatgattgc tatgtatgac aaaatgggag aagctagtga tgatgaaatg agtaagcttc
     2761 ttgaagaaac agcagagata caaactatac ttgaaaatag tggtttttat atgatagatg
     2821 caaagataca agaagttgca aatggtcttg gtcttggaga aattggtctt gataaagatg
     2881 taactgactt aagtggaggg caaagaacaa aagtacttct tactaaactt ctacttgaaa
     2941 atccaactat tcttatattg gatgagccta caaactattt agacgaagag catataacat
     3001 ggcttactaa gtatcttcaa gagtatgaga atagttttgt cttagtttct catgatattg
     3061 aatttataaa taatacatgt aatgtaatat atcatatgga aaatggagag ttaaatagat
     3121 ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat aaagaaacgt caagaagaac
     3181 aagcttatga taaacaagtt gaagaaagaa aaagactaga ggattttgta gctagaaata
     3241 aagctagagt agcaactaga ggaatggcta acagtagaca aaagcaactt gataagatgg
     3301 aaatattaga aagaccaaaa gaaaaaataa aacctacttt tgcttttaaa gatgctagag
     3361 cagctagtaa aataattttt gaaacagaag atttagtttt agggtatgat gaagcattga
     3421 caaaaccact taatttccat ttagaaagag gaaaaaagat tgccttaaaa ggaatgaatg
     3481 gtattggaaa atctactcta ttaaaaactt tacttggaat aataaaacca tttgaaggaa
     3541 atgttaaatt aggtgactat ttggaagttg gatattttga acaagagagt tcaagagaaa
     3601 atagtaatac acctatggat gaagtttgga gtgaatttcc agggcttaca aactttgaag
     3661 ttagacaggc tcttgcaaaa tgtggactta caaatgaaca tataacgagt caaatgagag
     3721 tattaagtgg tggagaagct gctaaggtta gactttgcaa agtaatgtta aaaaatataa
     3781 atttcttagt attagatgag cctacaaatc atttagatgt tgaggcaaaa gatgaattga
     3841 agaaggctat caaagagttt aagggaacag ttcttttagt atgtcatgag ccagaatttt
     3901 attcagaaat tgttgatgat gtatggaata tagaagattt tacaactaaa atcgtgtagt
     3961 gatttaactt attgctgggg ggatttcatg aaaaagtttc taaatgtact tttttcaatt
     4021 tgtattagta ttgttataat agtaggtgtt attaatttta ctgttggatt taaacaactt
     4081 tattattttg acattgatta cttaaatata tcagagttat ctggtttatc taaagatgat
     4141 ataaaattga actacgatta tctaattgat tataacttaa ataagaatgt gtctgagttc
     4201 aaattaccaa cattaaaatc ttctccacaa gggaagatac attttgaaga agtcaggaat
     4261 atatttcaaa atataaataa attagctaaa ttattattgg tagtttcttt agtaggaatt
     4321 atattgagtg taaaaaataa aaatataaaa atcttaaaaa ctacatcaat aacacttata
     4381 attatgccat tattattaac agttccaatt ttattaaatt ttgaaaagtc atttattatt
     4441 tttcataaat tattatttag aaatgattat tggatattta atcccgattt agacccagtt
     4501 ataaatatat tgccagagga attttttttt cactctggaa tgatgatttt aatattaatt
     4561 ttgttagtaa gtatagtact gtttgtaatg tataaattat gtaaatcttt atcaataaaa
     4621 atatttcaat gttaaataga gggatatttt tatttccaaa ataagataat aaaaaaggat
     4681 tagattaaaa gttatacaat aaaaatagct gtgcaataat atttagacta tgaaagtagt
     4741 taaaaaatat ttatgcacag ctatttataa tagaatttac tttttactag tgattttaac
     4801 taaatcttga aatttactca taatgtaatt gtaattatct tttttatgag gtaatataca
     4861 actactgcaa tctttgatac ctttatctgt atacttaaag tttccaccac agttttctcc
     4921 taaagcatac aaaggacaat agcaaaatag acagttaaac tcttctggtt tattagtttt
     4981 atgacaaggg aaaaattcgc aatctttgtg attaaaaaac ttataatttt cgcccatatt
     5041 ttcctcctaa ttaaataaaa tttaaatttt ttatactata taagattttg gtgataatat
     5101 aattatacta aataatttta tgaatagtcg ataatatagt attattaatc atagtattaa
     5161 tggtataatt agtcaacaga aaaataatgt aaaaataatg taagtgtatt ttgtaattag
     5221 aatatgtttt ataacaaaaa atgaggtgat taagtgaaag aaattcaaga aaaagcactt
     5281 cttgtaggtc tgaatttgac tacaatagtg aaaaaaaatg atgatataga cactaatgaa
     5341 tctatggaag aattaaaaga actaacaaaa gctgctgggg cagaagttgt aggtagctta
     5401 attcaaaata aacactctgt agatgcagct tattatatag gaaaaggtaa agtagaagaa
     5461 ataagagcct atagtgactc tttagatgct actttagtaa tttttaatga tgaattatct
     5521 ggtgctcaaa tcagaaatat agagaatgtt gttggaagaa aagtaataga tagaactact
     5581 ttaattttag atatatttgc tcaaagggca cttagcaaag aaggtaaatt acaagttgaa
     5641 ttagcacaat taaaatatag attgccacga ctttatggaa tgggtggtga gatgagtaga
     5701 acaggtgcag ggattggtac tagaggtcct ggagaacaaa aactagaaat agataagaga
     5761 catatactta ataaagcagc agatataaga cgtgaactta aagaagtaaa aaagaataga
     5821 gagacacaga gagttaagcg tttaaagtca aatataccga ttgtagcact tgttgggtat
     5881 acaaatgcag gaaaatctac cctgttgaac gaattaataa agacacacaa ggattatgaa
     5941 caagaaaaag aagtttttgt aaaagatatg ctttttgcaa ctttagatgt aaccttgaga
     6001 aaggctcttt tgccgaataa aaaagaattt ttagtagtag atactgttgg atttgtaagt
     6061 aaacttcccc atgatttagt agaagcgttt aaggcaacat tggaagaagt acagtatgca
     6121 gatttaatct tacatgtaat tgatgcaaca aatactagtt atgaattgca aaaaagtacg
     6181 actgaaggtg tattaaaaga acttggtgta aatgataaaa aacatatact tgtatataat
     6241 aaagtggaca agctagaatt ggatatatac cctaaaagtc aagaagatat tgtgtatatt
     6301 tctgcaaaac aaggcataaa tatggataag ttattaaata tgatagaaat tgctttaatg
     6361 gaaaatactt attcagtaag tttgatgtta ccttatgaga gaggtgatat atttagtaga
     6421 ataaaagata aatataatgt ggagaatttt gagtatgggg aaagtggtat aacgctagat
     6481 gtagatttag atgaagaaga ttttaatatc tatagagagt acatactaga aaaataggtt
     6541 gaatatggat ataaactggg aaaatataga taatcttgaa gattatttta taacctatct
     6601 tctttataaa gaatctaaaa cagtatctca aattagtaaa attagaaata tttcaagtac
     6661 tgaagcaagt gagcaactta tacaagcaaa gttaaaaata aaggaaatgc aaaaagatga
     6721 ttttgaggca tctaaggata ttttggataa atttctagaa ctagataaaa ttaagagact
     6781 tgattttatg gatagcttag atgacgaaaa aatggtatac tttaagagaa aggttttcaa
     6841 aagaatcctt gtagaaaaaa atgctgaaga tttaattgtt ttaatttggg cgacaggtga
     6901 gctaaaagat gataggtttt tgaaattgct tcatcagctc acaaaccaca gacactcaga
     6961 tattagaaga ataacttatt cagcaatcag aaagattgaa tcaccaagta gtagagaagt
     7021 actgcaaaaa ggtctctacg acaaaaatgc acagactagg cagtactgtg caaaagctct
     7081 ttcaaaattg ggagatgaaa acagtttaaa gattttacag cagttgaaag acaagaataa
     7141 gaattttgaa aaagaatatg tgcttagagc ttatgacgaa gccattaagt cattggaaaa
     7201 tgcaaaaaat ttgaaaaagt aattttttat taaagcaatt tgtttgctta atgatgtatt
     7261 tctaaagttt aacaaggtaa taaataaatt aatgggaggg gtttcaaatg atagtaagac
     7321 gttgtgcagg tggagtagtg ttttatgcta ataaagtact tattgtaaaa aatgataggg
     7381 gtgaatggac acttccaaaa ggaaagatac taggaggggg acttccatat gagagtgctg
     7441 ttcaaagagt taaagtagaa actggcatag atgctaaaat gatagatgtg gctggcgata
     7501 ctatgtatga atttttctca agaagtagac aacaagaagt ttgtaatgca ataatgtggt
     7561 atgttatgga agcttgtaac actgattgtg tacttgctcc tgaatttcaa gaaggtggat
     7621 tttataaagt aaaggatgct ttagaaatgt tatcacatca taaagagcaa gctttagtag
     7681 aagtatctta taaaaagttt aaggaattaa agaaattagt ttcagagggc gatgatgttc
     7741 cagcaaatta attgtgaatt tagaggattc tgaggaaggg atagcccctt ccttttttaa
     7801 ttgaagcagt tttaagcgta aattatatag tctctttaaa ctatgattta tcaatttaga
     7861 gatttagtag tttatattta aaatcagtaa gatatattta aaataaaaat taaaaagtat
     7921 acaattttat tatataattg tcttatgatg ttgattaaag aattattaaa agtccattta
     7981 tttttaaata gaataatttt ataaatatat atttgatgtg tatattagaa aatgattaaa
     8041 ataggaggaa atacaatgag caattacagg acattacatg aatttggaac ggatgaaata
     8101 acgattgaaa agtctgtttt tattggatat gctaagccta taaagagtga agaagaagca
     8161 ttagagttta taaatgaaat aaagaaaaag cataaagatg caactcacaa tgtgtgggct
     8221 tatactgtag gtaaaaatat gaatatacaa agatatagtg atgatggaga accacaagga
     8281 acagctggga tacctacatt agaggttata aaaaaagaag atttgagaga tgtagcggtt
     8341 gttgttacta ggtattttgg aggaacaaaa ttaggtgcag gtggtctagt tagagcttat
     8401 acaaaaggtg ctaaactggg acttgaagca ggtaagataa tttataaggt aatgtatcaa
     8461 gaagttaagg ttaagataga ttatactcaa cttggaaaag tgcaaaatga gttaatgaat
     8521 ttggggtatt ttataaaaga tactgtatat gaggataatg ttgaaatagt agtatattca
     8581 agattagaag atgtagagaa attatcagaa aaaatgattg atataacaag tggaacagga
     8641 aaaatagttt taggagagga attttatttg tctgaacaaa atggagaaat tttaatataa
     8701 tctaaaaggt tattagaata gttaatacct caaatagcca attagtattt ttaagtgaaa
     8761 agccattaaa ttgatttaat ctaaatcaat ttaatggctt tttatcggat tttgcacaga
     8821 atcctttttt tacttcaaat aaaccgacta aaagtaaaaa tatcccaaat atttttttta
     8881 atatatctga agacagataa ttagctaata gtgaaccaaa tacagctcct aaaaccccaa
     8941 acacagctat aggtataacc aatttaaaaa ttacattttt attttttata tgtatagcaa
     9001 gtgctataag tgtcataggg atagatgaaa gtaaatttac gctttgtgct attttagtgt
     9061 ctatgccagc aagtaaaacg agtgcaggta taaggattgt tcctccaccc attcccattc
     9121 ctccaataat tcctgcaaaa aatccaatta gagcaaataa cattaaaaca ccatccttaa
     9181 tgctgcaatt atcataatta ttccaaaaga aattctaaga aactttccag taaccttatt
     9241 tagtaattta gctcctataa taccaccaag aatacttcca actgcaactt taattgttaa
     9301 gttaacatca taagtacctt tagatacata taaaacagaa cttgctgtcg ttaagaatat
     9361 tattatagaa agagcagttg catgagattt atgttcttct accttaacga tgtcatttaa
     9421 tattggaact aaaagagtac ctcctccaga accaaaaact ccatttataa atcctgtaaa
     9481 aactccaatt atcgtatttt ttaagttaaa atcaaagtat ttttgcttta aatcatcagt
     9541 ttttttcata aaaatcacct tctgaaatag ttttaccaaa ataggcaaat tatatttata
     9601 taaaataaga ttaaatataa tctttcatgt ttgtttaatt ttgcataaaa aaaaacatat
     9661 taaaacaaca tattaatata tggtataata tattaaaaca aagtagcaaa tttgagagga
     9721 gataacaatg tcaaatataa aaatacaaat agacaaaact gtagaatggc ttataaataa
     9781 ggttaatgag gcaaatgcaa aaggattaat tgttggtgta tctggaggaa ttgattcggc
     9841 agtagttgct aatttaatta aaaaggcatt tccagaaaac tctatgggag ttataatgag
     9901 cataaaaagt aatcctcaag atagagaaga tgcacttaag gttatagagg gatgtgatat
     9961 agaatacctt gatttagacc ttatagaacc acaaagtgct atattagaca tggtagtagg
    10021 aaatttaaaa gataaacatt tatatagaga agaatattta aagatgacag atgcaaactt
    10081 aagagcaaga gtaagaatga gtacaattta tacaattgct aacaatctag gatatctggt
    10141 tgttggtaca gacaatgctg cagaaataca cacaggatat tttactaaat ttggtgatgg
    10201 tggagttgat attttaccta ttgcaaattt aactaaagga gaggtgtatg aatgggcaaa
    10261 agagcttggt gttcatgaag atttaataaa caaagctcct tcagcaggtc tttgggaagg
    10321 tcaaacagat gaagatgaaa tgggaactac ttacaatatg atagatgctg tattagaagg
    10381 tagattggat gaagttccta aaagagacca agagataatt gaaagacttc atagattaag
    10441 tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc taatttacta tttataagat
    10501 ttgtgataaa atttacaagt gatgaaaatt agaataatta attgtacgta ttaaggagtg
    10561 gaaatatggg acgtataggt aatataatta atagaaaagg taaacaagat gctcaaagag
    10621 caaagatatt tactaaacat gctagagcaa tagcagttgc agctaaagaa ggtggagcag
    10681 acccagagta caatgcagcg ttaaaaacag ctatagaaaa agctaaggca gataatatgc
    10741 caaatgataa tatagataga gctatcgcta aaggtgctgg agcaggtgct ggagaagact
    10801 atgaaactat agtttatgaa ggatatggac ctggtggagt tgctgttata gtagaaactt
    10861 taactgacaa caaaaacaga actgctggta acgtaagata ctattttgac aaaaacggag
    10921 gaaacttagg aactagtgga tgtgtgtctt ttatgtttga taaaaaagga caaatattag
    10981 ttggtttagg agatggagtt tcagaagaag aattgatgga tgttgcatta gaagcaggag
    11041 ctgaagattt cataacagaa gaagatggtt atgaaataat aactacacca gaagattttt
    11101 ctagtgttcg tgatgagtta aaagctaaag gatatgagtt tatatctgct gatgtgaaga
    11161 tgatacctca aactacaact gtattgactg aagaatctca tttaaaaatg atgaataaat
    11221 tagttgatat gcttgaagaa gatgatgatg ttcaagatat atatcataac tgggaagtag
    11281 aataaatagt ataagcatat tgaaatttat aagtatttaa tcaattttaa catgtgataa
    11341 attttataga ataagaaaca ctaaaataga catacgcacc tctaacctat atttgcataa
    11401 taatatgtaa gcataggtaa ggggtgtttt tatatttata ggttaagaaa tttaaagtaa
    11461 attttgagct ggactcgatt gtctataaat aattgatggg tatataactt gttgctgaat
    11521 accatttata cgcccagtta ctgtatcaat aatcatccta agactacgtt ctgctgtctc
    11581 ttccattttt aaatctataa ctgtcaaagg aggaatactt cttgccatta gtcgtgaagg
    11641 tccattactt gctgcaaata tttgaatatc ctctccaatt acaattcccg attcatgaaa
    11701 tgcattcatc ataccaagag ctaccacatc atgattacaa aaaattactt tttttagtga
    11761 attttttgca agcatttttt gagcaatatc atatccacta tctattttat tagaacaata
    11821 atatatagaa tctgatagat gtattccatg ttctttacat gtttttaaaa aagatttacc
    11881 ccttaaagtc atacatgtaa aggaagttgg gttgaataca acagcaatat catttttacc
    11941 attttgaata gcatgtaaag ctgctatacg accagtttcc tcattatcaa tagatacact
    12001 tgaaaaacca ggtatttcac gatttacaac aactacagga attttaggtt tattttttac
    12061 tagatattct atatcatcag cattgatacc tgttaaaaca attccatcat aataattatt
    12121 tgcaaaaaag tattcatcat cacatagatg accaactttg taaggtcgta tgatgatatt
    12181 tatgtttaaa tgttctaaag aaattatgtg gtttattgct tgaagtatat tggttgatgc
    12241 caactcaaat ccctcatcct gccaataaaa agctatttta atattattat gtttaatagg
    12301 agtagagcgt ttatatccaa gcttatcagc tgaatttaat ataagctctt gtgtatgtag
    12361 cggaatcctg cgttcatttc ctttaccact taagacgtac gagacagttg ctgaagaaag
    12421 accacagtct tttgcaatat ctttgattgt tgccatgata aaatcctcct taaatagttg
    12481 cttaaatttg cttataccaa taatcttaac atatattcaa ctaaagaaca atattctaca
    12541 aggtgcatat tttacaggat tagaataaat aaattaaaaa aaaacttctt acttatgttg
    12601 acataattta gtttatagga ttataatatg cttaaataag cttatgatta acttaaatta
    12661 agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgtaaa gggggaaaag
    12721 gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa aacctgaaag
    12781 agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct tcagggatta
    12841 aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg aaagatgcaa
    12901 aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat gcattagttc
    12961 caaatttata cggtgctaaa gttgcatggg agtgtggtat aagagatata tcctatgtta
    13021 tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa gagtctattg
    13081 atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt ggaatggcta
    13141 ctgtatttgg atgtcctttt gaaggtttac caaaaattga agatgccata agatttgcta
    13201 aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata gggattgctg
    13261 accctaagca agtaagagaa actattagtg cattagttga agctttacca gagtgcagat
    13321 ggcaagtaca tattcatgat actagaaata tgggaatggt taatactctt gtggctattg
    13381 aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc ccatttgctc
    13441 ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa cgtatggggt
    13501 atgatacagg agttgatttt gttaaacttc ttgaattatc aaagtatcag aagagttttg
    13561 taaatggagt atatagtggt catcatataa atataaagtc tgatagttgt aattgttagt
    13621 tctattgatt aatcgattaa aaaaatatgt tgattaaaaa aatatgctga ttggtaaaat
    13681 agatttattg ataaaaaata taaattataa aatacattat ctaataaaac aaataaaaaa
    13741 attgaggggg attagtatgg atattatagc tttactttta tttttaggag ttattattat
    13801 tgcatttgta cgtaagaata atgcaggtat tttggctcta gctattggtg tcatagctgt
    13861 tagaattttt ggaatggatg ataaagaact ttatggagct ataagcaact caatgtttac
    13921 tacacttgtt ggtataacac tactctttgc aatagtgaac tcaacaggag cattaaagct
    13981 tgttgcagaa aaaattgtag ctgcttctgg taaacgtgtt tggcttatac ctatagcagt
    14041 ttttatagca ggatttgttg ttgctggtgt tggacctggg gcaattccag cacttgcaat
    14101 tatcccagca atggctgttc ctgtagccat aacagttggt tataatcctg taatgcttgc
    14161 acttataggt gaatgtggat taatggcagg tcgtatgact cctattactc cagaaggtca
    14221 aattataaca accgttgcag aaggggttgg aattgaaaat gttatgccta ctatcttagc
    14281 ctgtcaaact ctttctacaa ttgtattttc aatagtattg tttataattt tcaaaggata
    14341 taaacttaaa aaacctataa atgttataga tagtaagcaa atagaaaaat tcaatataaa
    14401 gcagataatt tctctaataa gcatagtagt tatgattgct tttatcataa tttttgatat
    14461 gaatgttggg cttgcagctt ttgcagtttc agctgtactt atttttttca atattgctga
    14521 tgaaggagaa tgtatcaaat caattccatg gagcacgata gtaatggttc ttggagtagg
    14581 tgcaatgatg acaatcgtag atgcagcagg aggtattgac ttgatgagta atgcattatc
    14641 atcactgatg aatagtaaaa cagctactcc aattatgagt atttcagcag gacttatgtc
    14701 tatggttagt tctgctttag cagtagttta tccaactatg atgcctatgt gtgttgatat
    14761 agctaaggca gttggaggag tagacccact agcacttatt gctgcagttg gtgttggagg
    14821 ttctcttgct ggtgtaagcc cactttctac tggtggggca ttgattttag cagctatggg
    14881 ttcaagtaag aaagacttta ataaggaaga acaaaataag gtttttgtac agcttttcat
    14941 tatgagtgca gttggcttat tggttattgc agtagtatca atattatcat ttaatgcaat
    15001 aacacattta atgaactaat tagaataact gaaaataaat cattaaattt aacttttaat
    15061 attaatcaat ataatataat ttggagggga agttatgaaa aaaggagcat taagcaatat
    15121 aaaggtactt gaccttacaa gagttcttgc aggtccatat tgtacaatga tgttggcgga
    15181 ttttggagct gatgttataa aaatagagat gccaagaaaa ggtgatgata caagaggttt
    15241 tggacctttt gtaaatggag agagtttata ctatgcaaat ataaatagaa ataaaaaagg
    15301 tatcactcta aaccttaaat cagaagaagg aaaaaatata tttttagaaa tggtaaaaca
    15361 tgctgatgtt gtagtagaaa attacagacc aggtgtaatg gaaaagttag gtctgggtta
    15421 tgatgagttg gctaaagtta acaatcaaat aatttatgga gctgtatcag gatttggatg
    15481 ttatggacca tattcagaac gtccaggtta tgatattatt gcacaggcaa tgggtggtct
    15541 tatgagtata acagggcaag aaggaggaga accaacgaga gttggtaatg ctatgggtga
    15601 tgtactaggt ggaatgaatt taactattgg tatactcaca gcactcaacg ctagaaaaat
    15661 aataggaaaa ggacaaagag tagatgtttc cctcgttgat tctgttgtat cgtctctcga
    15721 aactggtata cagagatact ttgattcagg acaacctgaa cttatgggta atagatatgc
    15781 ttcagcctat ccttatgact cttttaaggc aagtgatgga agttttgtaa taggctgtgg
    15841 taatgacaaa ctttttgaaa agttatgtaa aaaagttctt tttagagaag accttttaat
    15901 agatgagaga tttaaaacaa atcttaatcg ttgtgaacat tacaaggctc ttaaaccaga
    15961 aatagaaaaa tggtctgtta agcatacaat aaaagaagca gttgacttaa tatcagaggc
    16021 tggagttcct gctgctccta tctataatct tgattgtgtt acaacagatg aacatatagc
    16081 aaaagcaaga gaaatgattg tagatattag tcatcctatt ataggaaata tgaaagtcaa
    16141 tggaaatcca gttaagttga tgacaacaaa agctaaaatt gatatgccag caccatctct
    16201 aggggaaagt aacgaggaga tttataatgg ttggcttggt tttgatagtg ataaaataaa
    16261 agatttaaaa gaaaatggag ttatttgatt aaaaaatagc taaacacact tgtgacttta
    16321 gtcatgggag gttcagttag aataatatca aaaaatatta aatcaatact gtaaatcaaa
    16381 tatagaattg tcagaagcat ttaagatggg aaaagctact tagacattga gtctttgtgg
    16441 cttttcctat tttcattaag aattttatat tatcagaata gatatgtact aaactaaagt
    16501 attaacaatt taaattaata gaaaataaac tacctaatta tgttgaataa agactacttt
    16561 agtgaaaata aacttataga gatgctattt aaattatttt aatggaaata tcaaaaatga
    16621 gactgcattc tcaaaaatga gactgcgttt tttaagtatt ttctcgaaaa tgagacaaaa
    16681 atattgtttt atgggtttta ttgaagaaaa attcttctgt gaattacaat ttaatcaatt
    16741 ttaaagttga aaattattaa aaatttagtg aaatttatgt atatttcgtt tattttatta
    16801 aaaataacaa ttttttacta aaaataaaga acttcaatgg tgattggcat agtacttgct
    16861 attttatatt aacaagaaat gtagaaaaaa taacaatgta taatgggggg aatatgatgg
    16921 aatctgtaat ggaaaatttg aacaatctta aggtggagct taaggacaaa gtctgtgtta
    16981 ttactataaa tagacctaaa gctttaaatg ctcttaatag tgacactctt agggaactat
    17041 cacaagttat tgatgtagta tcagaaaatg aggcaatatt aggagttatt attactggag
    17101 aaggtaaagt ttttgtagct ggtgctgata tacgccagat gcaaaactat aaaagtgagg
    17161 aaggcagaaa atatgcaggt tatgctcagg gaatttttga taaaattgaa gctctggaga
    17221 aaacagtaat agcagcagtt aatggatatg cattaggtgg tggatgtgaa cttgctatga
    17281 gttgtgatat tagaattgca tcagaaaagg ctatatttgg acaacctgaa gttaatttag
    17341 gagttattcc atgttttggt ggaactcaaa gattatcaag acttgttgga actggaatag
    17401 ctaaagagtt aatttttacg gggagacaag taaatgcaga agaagctaaa tctattgggc
    17461 ttattaataa agtggttcca agtgatttac ttttagaaga gtctatgaag atgatgaacc
    17521 aaatagttga aaaagcgcca atagcaataa gatatgcaaa agttgttatt aacaaaggga
    17581 tagatatgga tttaaagaat gctcttgaac tagagaaaga tattgcaggt ttaacttttg
    17641 ctacaagaga taagcaagaa ggaatgaatg catttataga aaagagaaag ccagtttttg
    17701 aaaataagta gctattataa ctaaaattgg aacaatgtag aatatattca aaatctaaat
    17761 ttcgtgaaaa aatacatata gtttaatttt aaaaggagtt aatttatgaa tacgggagta
    17821 attataagtt tagtagggat tatcatagct ctatctcttc tagttgtaat ggttatgaag
    17881 ggaataaata tttttattat agcaattgta tgttcatcat tagttgctat aacaggaagc
    17941 ctaaatgtgt atgatgcact taaagtggat tatatgacag ggtttgttgg atttcttcag
    18001 gcaaatttct ttattttcct aacaggtaca ttaatgggaa aaataatgga gattactggt
    18061 ggagcaaaat ctattgctaa gatgattgta agatggattg ggaaggataa agcactactt
    18121 tctattccaa tagcttgtgg aatattagcc tatggtggag taagtgtttt cgttgtaagt
    18181 tttgcagtgt ttccgatagc tttagaaatt tttaaagaag ctgatttgcc acgaagattt
    18241 ataccagcag ctttgacttt tggatgctca acttttgcta tggttgcacc aggggcacca
    18301 caaattcaga atattgttcc agcatcaaca ttagggacag atattatggc tggagcagta
    18361 aatggtttta tatcatgtgc agtaatgttt atagttggaa gtacaatttt gtacaaaatg
    18421 gtttcaaaag aaaaagccaa tggtggtcat tttatagcac atgagtcaga tatatttgat
    18481 gaagcagaag caactaattc taagagagaa aatggtccca atggtgtaat tgccttaatt
    18541 ccattgatag tgtcaatttt aataattaat gtaaaagtta atggaaaagc gatagtacca
    18601 attgaggtgg gagtatttat tggagcttta ttagtatatt tattgttaaa taaatatcaa
    18661 gataatacta aaatagttgg acatgttggt gatgcatgta aaactacagt agtagcaata
    18721 tgtaatactt gtgctgtagt tgcatttggt tcagtagtaa aatcagcagt tggttttgac
    18781 ttcatagtta atgccatgac taatattcct ggtcctccta ttgcaggggc tgcagttgga
    18841 actacagtta ttgcaggtat ttgtgggtca gcttcaggtg gactaggaat tgcagctcca
    18901 cttcttggac cagtattttt agccaagggt gtttccgctg cagcattaac taggactatg
    18961 gcaatttcat cagcagcatt agattcactt ccacacaatg gatatattgt tactgtaaca
    19021 aatggtcttt gtaaagaaac acataaggat gcctatatgc caatattctg ggtaactgtt
    19081 ttaaccccat ttattggaac catagtagca gtcctactgt ttacaatgtt cccgggattg
    19141 ccataagaga aaagataaaa ggtataaatt atattttgat attataaaat caattttcaa
    19201 aatatataca ataaatttca tgttttaata ctgtaaatat gtatttaaaa taacaaattg
    19261 accatataag gaggaattga aagtgttaaa taaaccatta gagggagtta aggttattga
    19321 cctaacgtat tttgtagcag gtccagggac ttctaaaata ttagctgact ggggtgctga
    19381 cgttattaag gtagagccaa gctttggaga cccaggaaga aagacagggg caacaatgac
    19441 aatgccaata gatgattata ataatccttt ttacagcaca tataattcaa ataaaagagg
    19501 tttgtctata aatcttaaga gtgaaactgg aattgaaatt atggataagc ttttaagcga
    19561 agcaaacgta tttgtttcaa gttatagaac aggagcgtta aaaagactag gtcttgatta
    19621 tgaatcatta agtaaaaagc atccacacct tatctggggt cagataaatg gttttggcga
    19681 ttttggacct gctaaggata atgcaggatt tgatacagtt gccttttggg caagaagtgg
    19741 ggctatgctt gatatagcag aaaaagatac aagtccaata aatccaccta tagcctttgg
    19801 agatgcaaca acttcttgta gtttatctgg aggaatatgt gcagctttgt accagcaagc
    19861 taagactggc aaaggacaaa aagtaatggc atctttattt gggcaagcaa tatggaatgc
    19921 atcatcttta ttagcatcta cacagtttag tgatgagtat ccaaaaacta gaaaaaatgc
    19981 tatttcacca gtaataaatt cttatcgttg taaggatgga aagtggatat tcttaagtat
    20041 tttagagcat gaaagatatt ttgaggcact ttgtagatta tttggaagag aagacttaat
    20101 aaaagaagag aaatttgcat cttcagtaga aagtaagaaa aatgccacag aacttattag
    20161 tattttggat ggtgaatttg caaagtttac tcaaaatgag atggttgaaa aacttactgc
    20221 tgctgatata gctcatgaaa aaattcaaca tgttaatgat gtagtgactg atgaacaggc
    20281 tattgctaat aactatatat atgaacatac aagtaaaaat gggaataaga caatgcttgc
    20341 atctactcca gttaaatttg gaaatattga agttaacatg acttgtgatg ctccacttat
    20401 aggagaacat tcagatgaga tattgagaga acttggatac agtgattctg atatagaagc
    20461 tctaattgaa tctggaattg taacaatgaa taaagaagca tgtgaaatat gttaataaac
    20521 atgtgaagta tgttaataaa taggagggaa ttttatggat tttagattaa cagaagcaca
    20581 attaatgctt caaagagtag caaaagaatt tgcagagaat gaaatagcac caatagctgc
    20641 tgaaacagac aaaacaggta tttttccaag ggagcttttt agcaaaatgg ctaaaatagg
    20701 atttaatgga attggaacac ctgtagagta tagtggttct ggtggggcag atatagaaaa
    20761 ggtaattgtt gttactgaga ttgccaaaaa atgtgcagca tctgcagcaa tattatcaat
    20821 acacacaata tatgctcaag ctattttgaa atttggtact gaggaacaaa agaaaaaata
    20881 ccttccaatg atggctgaag gtgggtgtgt aggtgctttt gccttaaccg aacctaatgc
    20941 tggttcagat gctgcgagag ctgcaacaac tgcaattatt gatgaagaaa ctgatgaata
    21001 tgttttaaat ggaactaagt gttttatttc tggtggaggt caagcagaat cattaataat
    21061 atttgcattg acagacccat caaagggaat aaagggaatg tctgctatca ttgtcgacaa
    21121 aggtactcca ggtttttcaa taggaaaaat tgaagaaaaa atgggtattc atggttcaga
    21181 aactgcagag ttaatatttg ataactgtag agttccaaag tctaacttat taggaaaaga
    21241 aggtaaagga tttaatatag ctatgacttg tttagatgga gcaagaattg gagttggagc
    21301 tcaagctgta ggaattgctg aaggggcttt agaagaaagt atcaaatatt ctaaagaaag
    21361 agtccagttt ggcaagccaa tttcagcttt gcaaggtatt caatggtata tagcagatat
    21421 ggcgacaatg gtggaatcag caaaattatt agtatattat gctgcagatt tgaaagctag
    21481 aggagaaaaa cacactaaag aagctgctat ggcaaaatac aatgcgtcac gtactgcaag
    21541 agaggttaca aatttagccc ttcaaatcca tggtggatat ggatatatga aggattatcc
    21601 attagagaga atgtacagag atgccaaaat aacagaaatt tacgaaggta cttcagaaat
    21661 acacaaagtt gttatttcaa gagcagtact aggataggag gaaaagaatg aatatagttg
    21721 tttgcttaaa gcaagttcca gatactaatg aagttaaaat aaataaagag acaggtactc
    21781 taataagaga tggtgtacca agtataatca atccagatga tagaaatgct ttagaagaag
    21841 ctctaaaaat gaaagatgaa ctaggagcag tgattaaggt tataagcatg ggacctcctc
    21901 aagctaaaag tgctttaaaa gaagctttgg caatgggagc tgatgaagct tatttaatct
    21961 cagatagagc ttttggtggt tcagatactt gggctacatc tacaattatt gctgctgcta
    22021 tagagaaggt tggaaagtat gatgtaatat tctgcggaag acaagctata gatggagata
    22081 cagctcaggt tggaccagag gttgcagaat ttttagggat accacaagtt acttatgcga
    22141 aagaagtaaa ggttcaagat gacaaattac tagtaactcg ttatacagag acaggggatt
    22201 atttaataga agcaaagatg ccagttttat taactgctat aaaagagtta aacaatccaa
    22261 gatatccaag tgtcaaggga atcttagaag cttataataa tggagatgca aagattactg
    22321 tattaacact tgcagattta gatgttgata caactcaaat tggattaaag ggttcaccta
    22381 caaacgttta taagtctttt gtaccagtta aagacaagca taatgaaata atagaaggta
    22441 taaataaaaa agaaaaagct gagaaactaa tagaaatact attcgatttg aaactagtat
    22501 aggagtgtta agatgatgag agcaaaagtt aatcaaggta taaatttaaa tgattataat
    22561 ggtgtttggg taataggaga gcaaagggaa ggtaaaatta atcctgttac gatagaactt
    22621 attggtgaag gaagaaagct tgctgaccaa ttaggaaaag agcttgcagt tgtgatagct
    22681 ggatatgaag tagagaaaga agtgaaggaa ttacttcact atagtgttga taaagtatat
    22741 tacatcaatg accctcttct taaagatttc actactgatg gatatgcaat ttcaattgca
    22801 aatttaatcg aaagaaaaaa gccagaggtt gtattggttg gtgctacttc tataggaaga
    22861 gatatagcgc ctagaattgc aggtaaggtt ggaacaggtc taactgctga ctgtacaaaa
    22921 cttgaaatag attcaacaga taataagtta ttacaaacaa gaccagcatt tggtggaaat
    22981 ttaatggcca ctattgtttg tccaaaaaat agaccacaga tgtcaacagt tcgtccagga
    23041 gttatggcaa aagctgtaag aaacgaatca gagactggaa ttttagaagt tgttactcca
    23101 gaacttactg aaaaaatgat tagaactaga ttagtagaga tacttccaca agaaaagaaa
    23161 tctgtaaatc taactgatgc tagaataata gtatcagggg gaagaggatt aaaaagagca
    23221 gaaggatttg agcttataaa agaattggct gataaattgg gggcagagat tggtgcttca
    23281 agagcagcag tagattcagg gtggatagaa cattctcatc aagtgggaca aacaggaact
    23341 actgttagac cagaactata cattgcatgt ggaatatctg gagcaataca acatttagca
    23401 ggtatgagtg attctaaata tatagttgct ataaataaag atgcaaaagc ccctattttt
    23461 agtatatgtg actatggaat agtaggagat ttatatgaaa ttatacctga aatgatagaa
    23521 tcattaaata ggtaactttt agaaaacatt gaatatgtag aatgataagt cttataatat
    23581 tgtattgtaa gacttattat ttttgctgaa ggttatagtg gggagatgaa acaatgaaag
    23641 agcagtggta taaggatata tttgcaagag tattatctat gactgatgat ggttttattg
    23701 ttgtaaatac aagtggagta ataatagata ttaatgataa atattgtgac tttttaggaa
    23761 aagaaagaaa agatataata ggacaaaata tacaaagtat tataccaaat acaaaaatgc
    23821 tagatgttat gaaaaataag tattgtgaag aaggagctat acatcattat tcaggtggaa
    23881 atacaaaaga aaaaagtgta atagtaagta ggtcttatgt ggaaaatgac aatggagaag
    23941 tagttgcagg agtagctcaa gttaaattta gattgcaatc atttgatgta gctaaaaagt
    24001 taatgtcaga atatatggag ctccaatatt ataaagagca gtttaaggat aactgtggtt
    24061 ttgacaagct cataggtgaa aatagagact tcatagaact taagaaaaca ggtgtgaaag
    24121 cttctaaaac aaactttcca gtacttttaa cgggtgaaac aggcacaggt aaagaagttt
    24181 ttgctagagc aattcacaat aatagcagta ggtcagataa acctatggtt agtataaatt
    24241 gcgctgcaat accagaagaa ttattagagt cagagctatt tggctatgac gaaggagctt
    24301 ttacaggagc aaaaaagggt ggtaaaaaag gtaaattttt agttgcgaat aatggtacaa
    24361 tctttttaga cgagataggt gacatgcctt tgaccatgca agcaaaactt ttaagagttc
    24421 ttcaggaaag tgagatagaa ccagtgggag gtcttaaaac tataaagata gatgtaagag
    24481 taatctctgc cacaagaaag aatttaagta aaatggttga agagggatta tttagagaag
    24541 acctatacta tagattaaat gtaataaaca ttcatatgat ggaattgaag gacaggcaag
    24601 atgatatttt attgctagca aattatatat taaacaaatt aaatgttgaa tacaaggaat
    24661 tgaaggtttt aagtgataag gttaaaaatt gttttattaa ctatacttgg ccgggaaata
    24721 taagagaact acaaaatgta ataaaaagtg cctatgctgt aagtgatgat atggtcatta
    24781 tgatgtgtga ccttccatct aagatggata atatcagcag agtagctcaa tgtaatgtgg
    24841 atagcaattg ttcaattcat gaaatggttg agaattatga aaagagctta attattgatg
    24901 tgttaaggaa gtataattgg aaatgttcta aggcagcaga ggttatggga atacacaaga
    24961 gtcttttata taagaaaata aaaaagtatg aaattgaatt aaataattaa gataacttga
    25021 attaaggcaa tgctaaaaaa gcgcccttat tcaaagcgaa ctgtataaac tgttttatgc
    25081 agttaaaacg tgtaaaagta gatgtgaagt actaatgtca atcattagta cttttttatt
    25141 atatttataa ttactatcta tatttcagta tcttttgata tctaagatta ttatgtttaa
    25201 tttagtatat aacaaaaccc tcttagttat ggacagtttt ttatatttta actgtctact
    25261 aagaggggat agtttagttt aaatttagag taaattttat ctagcattta taaaaaatat
    25321 tttgaatagc ttttttgtta tttagtcata attttctact tctttatcaa gttctttgcc
    25381 acaagaatca cacttatata tagcattgta tccgtaatca tattcatcaa ctattttctt
    25441 ttgtctaaat acaccaccac actcacatag gaaaccaatt tttataagtg taacgataaa
    25501 ttgtgatttt ataaatttta tatcattgca tttatcgtta gtgtaaaatg tataaatata
    25561 agagttatcg cactcatctc ttaattcaaa tctttcaata tatgtgttat tttttaattc
    25621 ctcaaattct tctttagtta aatatgattt ttgttgcaat tcatgtaaac gcttaatttg
    25681 gtccattatt tatatcctcc caataatttt agtattttaa gcttagataa ggaatctaag
    25741 ttttattaaa tttatcccaa ggaattattt aaatcatatt tagttttatt aattacaaca
    25801 tatataaatt acacgaaaat aaaactaatt gtaaaaataa tatcctgtga aaacctaaac
    25861 tactaacccc ttactaaccc gaaaactaaa aaaagaaaat atatccaaat ataagtgata
    25921 tataaataaa gatgtttcat ataaatttaa ctttaaatat tctgacaata atatatataa
    25981 atatattata gttaaaatat accacatatt ttgggtattg tatactggaa ttagagtaat
    26041 taatttattt tttaacatac aaatgatttt atgaaaaaaa acaagactca aaaattttta
    26101 tattctttga tagaataaaa ttgagagtaa atttattgaa ttttataaaa tttataaggg
    26161 gtgagtctaa gatatgcatg aagataaaaa tgatatattt gaatcaatag atactgagga
    26221 ttttgataat aaaacagaag aagaatgttt agagctagta agaaaaaatg gattgaattt
    26281 aatatacata aaaaatcaaa cagaagagat atgtctagag gctgttagac aaaattgtaa
    26341 tgcaataaaa tgtgttcaga atcagacaga aaaaatatgt atagaagcag ttaaacaaga
    26401 ttggagaatg ttagaatttg tggaagagcc aacagaagaa atttgcatgt tggctattaa
    26461 ccaagatgga acagcattaa aatatataga aaatcagaca gaggagttat gcttaagagc
    26521 cgttgaaaaa aatggtgcag ctttggagta tgtaaaagaa caaacagaag aaatatgtat
    26581 agaagcagtt agaaacagcg aatttggatt ggcaagagtg aaaaatcaaa cagaaaaaat
    26641 atgtatggaa gcagttaagc attgtagcta taatctaaaa tgggtagaaa atcaaacaga
    26701 agaaatatgt atggaagcag tgagacaaaa tgggcttgat ttgaaatttg taaaaaatca
    26761 aacagaaaca atctgtttaa gagcagtaag acaaaatgga atggcattag aatttgtaaa
    26821 ggaacagact gttggtatat gcttaaaggc agtaagacaa tatggaatgg cattaaaatt
    26881 tgtaaaagaa caaacagaag aaatttgtac agaagctata aaacaagata aaagagcact
    26941 tagttttgta aaaggagata aggaaaaata caaggctcta tatgataata atgaaccatt
    27001 tgcaaagaga tatgttagaa atgtaataga aaaagaaaac agagcattga ttaaaaaagg
    27061 agaagaaaat gcaaaaatca aaattgcagg aaaactttat gatagaggca tgtcttttga
    27121 agatatatca gatatagttg agattgaaat aagcaagtta aaagtgagtt taggtgtttt
    27181 ttaaagtaat aaatgtaggt gttttttatg ggaaatagtt aataaaaaaa taatcgacta
    27241 gctctttgta ggacagcaaa attagtgata taatttgatg tataataaat tatttttatt
    27301 agataagggg gaattaaagt tgaagattac agtattatac caaacaagaa gtggaaacac
    27361 agaaagggtc gctaagctga tagaagatgg agctaaaaag gtggatggag tagatgtaaa
    27421 gcttatgaga cttgataata ttgatttaga ttatttaaat gaaagtaaag cagtaatatt
    27481 tggaacacct acttatttag caaatacaac ttgggaagta aaaaaatggt ttgatgaaga
    27541 ttcaaaaaaa gttaatttag ctggaaaatt aggagctgtt tttgctacat gtgattatat
    27601 atgtggaggt ccagatgttg cgatacttac tatagtagga catttgatgg ttaaagggat
    27661 gcttgtgtat tctggaggtg gttctttagg aaagccattt atacatttag gacatgtaca
    27721 ttcaattgaa ggtccagagt tacaagatga gaaggctata atatttggtg aaagaattgc
    27781 tactaaggca aaagaactat ttgcttaatt tctatatgca taataatgat tagagtaaat
    27841 ttagggtttt aatggagatt tgaagtaaaa gttataaaat aaaaaatttt ccataaaatt
    27901 actcaagatg aattataata taattataaa taaattatat tattaaaata tttaaaatca
    27961 aacgtcaata gcgagggaag tttatggtat ggcatataga atatgagatt ttttctgcaa
    28021 ttatagttat ttttttaatg gtatactttt ttagaagtaa atttatacct actttacaaa
    28081 acaaaatata ttgtgcactt ttaatattct catttttatt tataatttca aatattttag
    28141 gttcattttg tctaaataat atagacaaaa ttccaatttt tattactttt ttattgaatc
    28201 agatatactt attgttattg ccaataccag cagcacttgt atcattttat gttatggcta
    28261 ttatttatca agatataagg tatatgaaaa aaagattatt atttttatct ataccactta
    28321 taatttgtat aatcttatct attacaaatc cttttacgca tattctattt ggattgtcaa
    28381 aagaaacagg ttatatacga ggttgtggat atgttgcaac ttttgcatct tttttctttt
    28441 atgcaatata ttcagcattt ttatctttta aatatagaaa agcaatgcat gaatcaaaaa
    28501 tatgggctat tagaggtttt cttatagttt caattgtggc aattatctta caggcaattt
    28561 ttttacagta tcttcttaca ggaactgcat gtgcatgtag tttacttttg acatatcttt
    28621 ctatgcagaa tcgaggtctt attattgatg accttactgg tgtactcaat agacaatctt
    28681 ttattcaaga gctagatttg aatatcaata ctgaacaatc aggatttatt attacaattg
    28741 cgcttgatga ttttaagttt atgaatgaaa cttttggaac aaaaaatgga gatattgcac
    28801 tagaagaagt tggaaaatat ttgattcaga tacatgatgt aaaccatgta tatagaatag
    28861 ggggagatat attttctatt gttgtcaata gtcaattagg tatagaacca gatgatatta
    28921 tcagaaagat agaagaaaga tttaaaaagc cttggctagt agaagagata agttttaatt
    28981 tagcaactag tattgctgtt gtttattacc cagaaaatgc agaaacgaca gaagatgtaa
    29041 ttgttgctat agatttctct atacatgaag caaaacgctc tgagtcaagg cgagttgttt
    29101 atgcagatgc gtctataagt gaaaagataa agaagaaaca tgttattaaa aattgtttaa
    29161 aagaagcatt aaaaaatgat gggtttgaag tgtactacca acctatattt tctaatagca
    29221 ggggcaggtt tacttcagca gaggcattac ttaggctaga acacaaagag ttaggtttta
    29281 tacctcctga tgaatttatt ccaattgcag aacaaacagg attgattaac tcaattggat
    29341 tagtagtctt tgagaaggta tgtagattta tagctagtga tgagtttgaa gatttaaaat
    29401 tggataatat agctgtgaat ctttctgtag tacaatgtat gcaaaaaaac ttagctgatg
    29461 atttacttta tcttatgaat aaatataatg tatcaccaac taaatttaaa cttgaaatta
    29521 ctgagactgt ggctatgggc tcttttaata taattaaaga aacaatggaa agattaattg
    29581 atttaggtgt taagtttgca cttgatgatt ttggaattgc ttattctgga gttactaata
    29641 tgctttcttt accattttca gtaattaagt tagataagag tttgatttgg tctatgaatg
    29701 aagatagtag gcataaactt acagttgaga cgattatagc acttataaat aaattgaata
    29761 tgaaggctgt agcagaaggt gttgaaacca ttaagtgtgc tgaagattta atagctatgg
    29821 agtgtgaata tttgcaaggg tattattttt ctaaaccagt tccagaagat gtttttaaaa
    29881 atttgttgaa agaaaatact tttacaagtt aacacccttt atttttttaa ttacaacttt
    29941 gttcattgac actgccagcg atatattata aaattaacac aataataaat attaaattta
    30001 ttatacatat tattaatatt aattaatgga gtaaagattt attatatatt ttatttaatt
    30061 atcctaaagt gagaaaggaa gatgtgacat gaataaaaag aaaatattag tgccaattga
    30121 tgggacagaa agaagtatgc attctttgga atttataaaa ggaatattta aaaaagatga
    30181 agttgaagta gaaataatga atgtaaaaga actagtattt attgatggca tatccctggc
    30241 tgaagagata aaaaattctg aaaatttagg aagaagaatc ttggataaag cagcagagat
    30301 aatgggagag tatgatgtta aagtacattt tacttttgga tatccaggag atgagataat
    30361 tagaaaagca aaagaagata atgtagattt tatagttatg acaaaatcta ctaaaaaagg
    30421 tcttacaaga atgataggtt cagtaacagc aagtgtagtt aaacaagcaa aatgtatagt
    30481 gatgattgta ccggagtaga attagaaagg tatttgaaat atgaataaaa agatagtagc
    30541 agtttgtgaa agtgaaagaa gtttaaattc tcttaagaca gctgcaaatg atttaggatt
    30601 tgatttagtt tatgaaattc aagaaaatga taatataaaa aatgaacttt ctatttatga
    30661 tatagaagat gcatccatag tgttatttgt tacaagtgat tctatagaaa gcatagagaa
    30721 gattgataga tttatagata gagaatttta tgaagttgat ccaaagtata taatagagga
    30781 tgcaaaatct atagtaagtg aaattttaat agacttaaat taatcgtggg tgtcgcacta
    30841 agaatagagc gacgcctttt tatatatttt acacaagaaa taaagaggtt gtatcaaaat
    30901 atagataaga ctctttttca ttgttgaaat gatgaaaata tatttgattt catacaaaaa
    30961 agagtacctc ataactaaaa agttcacttt gagacactcc atttttatat gttagttgca
    31021 tttaaggtaa aatataagtt taatcatgtt gaaataactg aattacatct tctaaatgat
    31081 tgtacattga tgtataacct aagtaagagt ctttactatc aaatgccatc attatcttgt
    31141 tatggtggct aatcatatcc ttgagagaat ttctatattt aaaaccgaag tattggttag
    31201 aagaaactgt tttaggtatt aactcataat attttataaa aattggatta tgagaagcta
    31261 aaattatctc tttgtgaaat tgcaagtcag catcataaca ttcttctatc aaaccatgag
    31321 aaaccttatc caattcctct attgacttta ggtaagtata ttttattttt tgaagttctt
    31381 tagatgtagc atttaaaatt gctagtttag tagcttcgct ttcgataagt tgtcttgctc
    31441 ttaaagattc tacataaaat tcttgtatgt tatgattttt atagatttca ccatcatcaa
    31501 ataggtcaac ttttttaaat ccttcaacta taggacttga ataaggcaca gctctaagta
    31561 ttccttcctt acagagtata tttatggcat ttcttacagg tattctactt atgttaaata
    31621 ggtcagcaat ctttctctca cttggtaatt tactattaat tttaaaagtt ccatttagaa
    31681 tgtttaattt aatataatca ataatttttt cagtggcatt taagcttttc ataaaaactc
    31741 ctaaacatat aattttttta tgattgtatt atatcatagt taagagttaa atctactttt
    31801 ttataatatg tataagacag gatagaggcc aaaatagcta taatagccaa gataaagtat
    31861 ccatattgta ttgaagtata ttccgatatt ttaccaacta tcattggaaa tacagacatt
    31921 cctatagcat aaattgcttg aaaaaaaccc attgcagtag atttctgatg ttttggtaca
    31981 tttttcatag actcgctagt aaggtaagaa aggagtatac ctgtagatat acctggtagt
    32041 acttgtagta caaatattat attaatattt tttattgttg ggactaaaaa gcaataggct
    32101 gaaactaaaa taaaaactaa aggaatccaa aattttggtc catacttagt acaaaattta
    32161 gtagaagcaa agccagaaga agcaacagca cacaccatgt aaaatataga agatagacct
    32221 atgagggtag aactagaacc taagttttta agaatttgag tagtaaatga cattgttgta
    32281 gacatttgaa taccctgttg aattaatgcc ataaaggaaa aaaatacaag tcttttatcc
    32341 ttgcagatag ataaatattt tttaattgat attttttcag gtattttttt tgaattatga
    32401 ttttttatat ttaaggctag aatcgttcca ataactgaag acaatacact catgatacat
    32461 aaggctgtca ttcctaattt ttcataaaat aaagtactca taacaaatcc aatcaacatt
    32521 cctagattgt ttaccaatat tatcgtactt gtagcttttt gttgatgttc tgtagaaaaa
    32581 aagttggtat agtgaaccat gtaagaaatc caagttgatg atgatattcc agataaaatg
    32641 ttagctataa gaaaaccatt gcctccagga gaaaagactc ttataagaga tgctaaacca
    32701 gcaaagaaaa ttccagacag tataaatatt ttatgtttat ttatagaatc agcaaatact
    32761 ccaacaggaa gccttaataa acattgtgat attccatatg cacctattat tataccaatc
    32821 atatttgtag atacattaca tgcacttaaa tatggatttt ggaaaggtat atatacatat
    32881 tgtgaaaacc agaaaaatgt aacaatagaa agtaaaagta atttttgatt agatattttt
    32941 ttcatattat cctccatatg gtatttttat aatgcaaata atataccatt tagatttttt
    33001 aatcaagtac tttctctatt aaaaaaaatt taaatatata attattattt acaaaaaaat
    33061 tatactttat agttgaatta agaaaacgct tatgatacta taaagttaac aaatttgaat
    33121 atataacagt agacgtgtta ctggtaatgc aggcaagatc gaatcaattt ttatagatta
    33181 tctatagagt actatgggta taatctattt atttggttcg atttttttgt ttaattggcc
    33241 ataaaacaaa aagcctatag ttgaaaatgg aggatttttt atggattata aaaatatagc
    33301 tcaagaaatt ctactaaatg taggaggaaa agagaatgta aatgaagtga ctcattgtat
    33361 gacaagactt agatttaaag tcaaaagcgc ttctaaagtg aataaggata aattatctaa
    33421 aactgaagga gtaataactg ttgttgagag tatgggtcaa atacaagttg taattggaaa
    33481 taaagtaaaa aaagtatatg acgaggttat aaaaattgta cctcaatcaa ataacgtagg
    33541 taaaaagaat gaaagtcaag aaaaccaagg tataattaat tctatattat cagcagtagc
    33601 tggtatattt acacctacaa tacctgctat agcaggggtt ggtatgatta aaggtatttt
    33661 atcagttctt gctatgtatt atatgaataa aaatggagta gatataaaag aaacgcaaag
    33721 ctatattatc ttaaatgcta tggctgattc tatattctat tttatgccta ttatactagg
    33781 atatactgcg gctaaagtat ttaatgcaaa taaaataata tctatggtat taggagctac
    33841 actttgttat ccaactttta ctgctttgat ggcaggtgaa gaatctgtta gatttttagg
    33901 attggcagta acaaaagcta cgtatacatc atctgttatt ccaataatta ttgctatatg
    33961 ggctctttca tacgttgaaa aagtattaga aaagtatata cctgagataa ttaaaataat
    34021 aatggttcca actttatcat tggttgtaat gttacctgca acattatttt tatttggacc
    34081 aatcggaatt tatataggaa atgtcataaa cttttcttat aagtatattt atgaattaag
    34141 cccagcattg tgtggagcat ttgtaggtgg gttatggtgt gtgttggtta tatttggtgc
    34201 tcatagagca ttattaccaa taggtataag tgatgttgct caaacaggaa gacagaatct
    34261 attggcgttt gcaggagctg caaacttttc acaagcaggg gctgctctag gagtattctt
    34321 taaaactaaa aatcaaggac taaaaacaat ttctatgtct gcaactataa cagctttatt
    34381 tggtataact gaacctgcaa tatacggtgc caatcttaga ttaaaaaaac ctatggtatg
    34441 tgccgtaata tgtggtgcat ttggaggtgc gataatggga atgggtggag cttatggtaa
    34501 tgcatttgca aaccaaggtg ttttaactat acctgtatat gctgaagcag gtgcattagg
    34561 tttcttaagt tatctaggtg gttgtgctat agcatttttt ggttcagcta tttcaactta
    34621 tctagttgga tttgaagatt tggaagagtc ttctaataag gaaaattcat ctataaaagt
    34681 agaaacgaag gatggtgtta ttgatataac ttcacctgta gagggcgaat gtatagaatt
    34741 gtcagaagta aaggatgatg tatttgcttc aaaagctatg ggagaaggta tagctgtgtt
    34801 acctacaaaa ggagtaataa cagcccctac tgattgtgaa gtagcttctt tatttccaac
    34861 attacatgca ataggattaa aattagataa tggagcagaa atgttaatac atgttggaat
    34921 caatacagta gagttaaatg gtaaatactt tacaaaacat gttaatcaag gagatttagt
    34981 gaaaaaaggt gataaactta tatcctttga tatagataaa ataaaaaagg ctggttatga
    35041 tgtaactacg ccagttatag taaacaatac atttgatttt gggcaagtag tttcttgtaa
    35101 atctagttat gtttcaacca atgataatat aatttcatta gtattagctg gtaattaagt
    35161 taatttgact agagataagt aaattgattt aaaaggaaat aagtaaatca acaattaaat
    35221 acatctaaaa gtaagtttat tgcaatgaat tgttaaagaa aataattaat atggagtaaa
    35281 tctattgcaa tgaagtaaca aagaaaataa ttaatataga gtaagtttat tgtaatgaat
    35341 tgttaaagaa aataattaat atagggtaaa tctatctcaa tgaaataaca aagaaaagag
    35401 ttaatataga gtgaacttat tgcaatgaat caacaaagaa aataattaat aaaaaggaga
    35461 aatatttatg aatacaggat ttccaaaaga ctttttattg ggagcatcat cttctgcatt
    35521 tcaagtggaa ggagcttggg ataaagacaa taaaggaaaa accgtagccg actataattc
    35581 ttttaaaaag tctcatttac aggcagatac taaagtcgca tcagactttt accataatta
    35641 tgaagaagat attgaattaa tgaaagaatt agggatgaaa acatatcgtt tttcaatttc
    35701 atgggcaaga ataataccag atggtgaagg agagataaac cagaaaggtt tagaatttta
    35761 taataagata atagacaaac taatagagtg tgatattgag ccattcgtaa ctttatacca
    35821 ttttgacctt ccatttaaat tagtagagaa atataatggt tgggaaagta gagaaactgt
    35881 ctatgctttt gaaagatttg ctaaaatatg ctttaagcat tttggagata gggtaaagta
    35941 ttggcaacct cataatgaac agaatttaat tgttagagtg gaggagagaa ttaatatata
    36001 tgatgaaaca gattcatgga agatagataa aatgcgtgct cagatggatt ataatctttg
    36061 cttagcacat gcattagcag tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg
    36121 agcagcagta tcatcatctg taacatatcc tcttacttct aagccagaag atgtatatgc
    36181 agctagaatg aatgataatt ttaaagtgta ctatatgctt gatatgcatc attatggaga
    36241 atatccaggg tattatatga agtatcttga aaaaagaaat atagttcctc acatggaaga
    36301 tggggataaa gagatactta aaaaagctaa aatggatttt atagctgtaa attattatag
    36361 gacaaattgt gcagaagctt tacctgaaga ttcacaacat tcatttggat taagagaagg
    36421 aactgttgat tttagtatgt atggactatt taaaatgtca atgaatccta acttagaggc
    36481 ctctgagtat ggagcagcaa tagacccatc aggtcttaga gttgccttaa atgaatattg
    36541 gcaaagatat cacttacctg ttataataac agaaaatggt ctaggagcta aagatatatt
    36601 agaagatgga aaaattcatg atgactatcg tatagactat ttaagaagtc atataaatgc
    36661 atgtaaacta gctattgaag atggtgtaga gatgattgga tattgtccat ggtcatttac
    36721 agacctttta agttcttctc aaggttttaa taagcgttat ggacttgttt atataaatag
    36781 aactgaccat gaaattttag atttaaaaag aatcaagaaa gatagttttt actggtataa
    36841 agaagttata gagaacaatg gaatagtaaa ataaacataa atatatattg agggttttag
    36901 tatgtacatt acaaaggtat taaataattc gttgctactt gcaaaggatg ataatgatga
    36961 agaaataata ttgatgggga aaggcattgg ccataattat aaggccgggt ctgagttaaa
    37021 taaaaaagat atagaaaaga tttttgttct tcatgatgaa aatataaaaa agagtattat
    37081 acaattggca acagaaatag atgcagaata ttttggtata gctcaaatga taattgcata
    37141 tggaatagaa aaatataact taaaacttat gaaccatata tatttggcct taactgacca
    37201 cattgccttt gcagtgagac gttttaaaga aggtataatg attgaaaatc actatttgtt
    37261 cgaaatcaag gaatttaatc caaaagagta tgatatagga aaatatgcta ttactgtttt
    37321 taaggaggtt cttggtttag acctaccaga agaagagatt ggtaatatag caggccattt
    37381 tataaatgca caacaagata atccttatag tgatagaaat aagagaagtg ctaaaattgt
    37441 aaatgctatt ttgcaaatag ttcattatca cttttctatt gtatataata aagagtcatt
    37501 ttattatagg agatttgtaa tgcatctaaa ggccttttca caaagatttc tggctaatga
    37561 accatcaaga gaaaaaatag attttatata tgagcaagtt caaaaaaatt gtaaggaaga
    37621 gtatgaatgt gtgaaaaagg taggagctta tatgtataag gaatactcca gagaattacc
    37681 aagacaagag gagttatatt tgatgattca tattcataag attttaggag aattatgcga
    37741 aaatagttag aatatcttaa tatgctagta atattataaa aaattaaaaa ttaactgcac
    37801 tttaaatatg aataattgca ttacttaagg gggttgtctt aaaattgatt tttttaatta
    37861 aatttgaagc tcaactcctt ttttaaacct ttatatcatt acaactttaa tgtttatacg
    37921 cctgtacttt agatgtatat ctacttttta catttctcaa catgcaaacc taccattttt
    37981 tgcagtatgt taattgaaat aaaccattga gttaacccta aatttattct ggacttatag
    38041 gaatagttcc aaagatactt taaaatattt tctggaataa ttgcttattt cacatatgtt
    38101 ttattgtgat gttttgattt aataaattct tcaactctgt atatagtaaa tgtattatca
    38161 tgtttgtttt attcaagcac acttataata cgcttgattt tttcacaatg atgaaatata
    38221 ctaatgggtg ttataggtat aagaatcgca ttacacttga agaagtaagt gataaatgag
    38281 atataaatcc tcatatgata aactactatt tcattgagga acatattcta agtgatgtca
    38341 agatactaca atatgactaa attcctagtg ataaaaaagt ctacgaatga gtgtagaaag
    38401 gggaactaag atgaataaag ttttaattat agatgatgat aaagatcttt gtattttgtt
    38461 aaaaaagagt atttcaattg aacagattga agcaaactat tgccattctg ggagagatgg
    38521 actggttgaa ctttcaaaac aagattatca gttagtagtt ctagacgtga tgatgccagg
    38581 tatggatggt tttgaaacta tggaacaaat acgaaaatat agcagtattc ccatcttaat
    38641 gctgacatca aaaagcgata attacagcaa agtgcatgga ttgcgagctg gtgcagatga
    38701 ctatctaacc aaaccatttg aaattacaga atttactgct cgtgtactgt ctttaatccg
    38761 acgttacacc cgttttaatc caaaaaatga ggagccacag caactttctt tcaaaggttt
    38821 gttgattgac cttgaccagc atacagttac taccaatggc gaacctatgt tgcttccacc
    38881 caaggaattt ggtgtacttc tttactgtgc aaggcatcag ggtaagattt tgacaaaaca
    38941 gcagatttat gaagccgtgt ggggtgagcc atatgtatat gatgacagca atatcatggc
    39001 tattatcagt cgcctaagaa aaaaaattga actagaccca tcatctccca tctatttgca
    39061 aacaataaaa ggaattgggt atcaattcaa cacggaggta tgaaaatgga tgaacaaacg
    39121 ataattcttt tcttactctt gctgtcgatt ggtctacttt ttctttccat atttgctttt
    39181 tccaagcttg ggcatattta caagcgccta aaagatatag aggagatact ggctgatgta
    39241 caaattggag acgaaaatcg taaaatacta attaagccat gtgatgttat ggctccattg
    39301 gtttatcagc ttaatgagat tgtctacgat tatgaaaata agttactttc tctgaaaaag
    39361 tcggataaag ctagcaaaca acttatgaca agtttatctc atgatgttcg tacaccatta
    39421 acaactctga ttggttatct ggatgctgta cacagtggta tagttatcga acaagagcgg
    39481 gaggaatacc ttgaaatcgc taggcgtaga gcctacgacc tcaaggatta cattgatgtt
    39541 ctttttgact ggtttcggtt gaactctgat gaatttacgc tgtcaataga atctgtggaa
    39601 attgcagagt tgtcacgcaa tattttgaaa gactggattc ctatattcca tgaaaagaaa
    39661 ctgaattttg aaattgatat tccagaaaat cgactaatgg taaacctcga cccagatgga
    39721 tattcccgtg tggtcaataa tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa
    39781 atcaaaatta ccatgagtga ggattccaga atggtattac tccgtgttga ggatgatggt
    39841 gttgggattg ccagagaaaa tttaccacat atttttgagc gactgtataa gtgtgataaa
    39901 ggacgttcag aaaaaggaag tggtcttggt ctatctattg tcagtcaaat ggtggagcga
    39961 atgggtggtc aggtttccgt agaaagtgag attggaaagt ataccgtatt tacagtgagc
    40021 ttaccattaa tataaaattg tttttccccc atgtgacatg tatacacagg gggatttttt
    40081 gtaagttata aagttgcaag gttaatacaa ggttgttgca aggttaatgt aagattgagg
    40141 tggtatgata actttaataa acaaaggagg cttttattat ttatgaataa tttaattatt
    40201 gaaacaaaaa atctcactaa gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt
    40261 cacgttcaga aaggtcgcat ttacggattg ctaggaagaa acggtgcggg taagacgaca
    40321 actatgaaaa tgttattggg tctaacaaaa ccaactactg gagaaattaa aatatttggt
    40381 caggacattc acaataacga gaagaagctt cttcctcgaa ttggatgttt gattgaatca
    40441 ccagggtttt atcctaatct gacaggaaca gaaaatctca aaatctttgc aaggcttaga
    40501 ggaataccaa aaaaaaatgc agtagaatca gcactggatg tagtcggttt gccaaacaat
    40561 gacaaaaagt tattttctca atactctctt ggtatgaaac agaggttggc gattgctctt
    40621 gccattatgc atgacccaca actgttgatt ttggacgagc ctatcaatgg acttgatcct
    40681 attggtattg cagaagtaag aacatttatt cgtgatctct gcacagaacg aggtaaaact
    40741 attttgattt ccagtcacat tctttctgaa attgccttgt tggcagatga cattggcatt
    40801 attgaccatg gtattttgtt ggaggaagaa agtcttgccc aattagaaga aaaaaacagt
    40861 aaatacatac attttgctgt atctgatgca gcacaggcag caaggcttct ggaaacccaa
    40921 cttaacctga aaaatttcaa agtagcagat aattacaata ttatgctata tgatacagat
    40981 gtttctgttg cagaaataaa tcgtgctttc attctaaaca acattgaggt atcagaatct
    41041 catttatgcg aagatactct tgaagattac ttcaagaaag taacaggggg tgaaggaatt
    41101 gcttgatata atttcatgtg aattttcaaa actcaagcgg ttaaaattta tactgatttc
    41161 tattttaggt gcttgcttat ttccaattcc cgcaacaatt ttgattgcaa aggataactt
    41221 gccctttgag caattgttca aattagttgt caattttgga tattttttgc tattaccaat
    41281 tgtattaagt attgttgcta gccaactatt ttttatagaa agagataatg atgtattaaa
    41341 aaatcttgca acagtgccag tgccaaaagg aaaactggct ctggcaaaat tggctgtgtt
    41401 gctgtttata tcattgttct actctgttgc agggttagga gctactatca ttggaggttt
    41461 cattgttgga atagtagagg gggttgctgt aaaattagga atgagcattg cttttggaat
    41521 tatgtttttt gttactgtac ttcctgttgt ggtactcatt gtatatttca atcgcagcta
    41581 tattttttct attatacttt cttttgtata tgctattttc aattttagta tatccttaaa
    41641 tattatcaat tttgaaccaa ataacccaat attaagtgta ttacctgctc ctgttataat
    41701 gcgttggtgg atggcatttt ggggagatcc cactggcgaa tacacggcac ttagacagcc
    41761 atatttgctc tcaactcctg cttgtgtagg tattttatgt ttgataacca ttatagcagt
    41821 cctgctgatt tgtatgatat acaaaaagca ggagaattag gaggtatcta tgcttgatat
    41881 aattactaca gaatttcaaa aaataaaaag atataatatt ctatggattg gtgttgttgc
    41941 tgttctgttt tcagcactat tggcagtttt tcaggttgta agttcacacg gcagtgaccc
    42001 tctaacgtat gagagctttg caaacggagt tatctggaat aatttcagtc ttagttttcc
    42061 atttgggatt acacttattg gaggatatct tattaaccgt gaatatacag accaaacctt
    42121 aaaaaacatc ctaactgttc ctatttcatt acgcaaattg ctagtgggaa aattaattgc
    42181 ggttggagga attactgtat tgttaagtct tttcagcttc ttatgcacat ttattttggg
    42241 gttgttattt tgtaatatca atatatctgt tacactcgtt gtaaaatcct tagttcagat
    42301 tttgtctgtt aatctgtatt gttatgttgc tgttttgccg attattgctt attttggtcg
    42361 taaacaaaat gcatttctga caggtgtagg gattgcattt gtatacggtt tttgtggtgt
    42421 gtttgttgct ggaagaaatc tgacagactt ttatccaatc acagcaggtc ttggtctagt
    42481 tggatacaac aatggtgctg gtactgttta tcagccactt atcggtgcta tgactttgat
    42541 tgtaattcta atcttgacaa ctattctact tgtatttaca cctaattatg ataaagttat
    42601 gaccatatct aagaaaaaag ggataaagca taaaaagtat tctcataaat aacataaata
    42661 ttgtttctat tatttaagtt gctagacagt gaaaagaaaa aatctgtcgt acagtggcta
    42721 tatgctcatg tattcaagaa gctacagttt tgatttttca taactgtagc ttctttttgt
    42781 atttattaaa tcacgactat cataatactt gcaataggta tagcgacaag agagagtact
    42841 gt
//
LOCUS       DAENCV010000025        42537 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAENCV010000025 DAENCV010000000
VERSION     DAENCV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.025"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(99..653)
                     /locus_tag="KQD82_001252"
     CDS             complement(99..653)
                     /locus_tag="KQD82_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG4379730.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILDK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(731..1096)
                     /locus_tag="KQD82_001253"
     CDS             complement(731..1096)
                     /locus_tag="KQD82_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379731.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            1560..2345
                     /locus_tag="KQD82_001254"
     CDS             1560..2345
                     /locus_tag="KQD82_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4379732.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(2442..3266)
                     /gene="pdaA"
                     /locus_tag="KQD82_001255"
     CDS             complement(2442..3266)
                     /gene="pdaA"
                     /locus_tag="KQD82_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG4379733.1"
                     /translation="MKKDSLKKYIMIGAFALVLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            3487..6606
                     /locus_tag="KQD82_001256"
     CDS             3487..6606
                     /locus_tag="KQD82_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG4379734.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIHTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKGGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            6624..7223
                     /locus_tag="KQD82_001257"
     CDS             6624..7223
                     /locus_tag="KQD82_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG4379735.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(7465..9594)
                     /locus_tag="KQD82_001258"
     CDS             complement(7465..9594)
                     /locus_tag="KQD82_001258"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG4379736.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            9860..10108
                     /locus_tag="KQD82_001259"
     CDS             9860..10108
                     /locus_tag="KQD82_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379737.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            10274..11665
                     /locus_tag="KQD82_001260"
     CDS             10274..11665
                     /locus_tag="KQD82_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG4379738.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            11776..13245
                     /locus_tag="KQD82_001261"
     CDS             11776..13245
                     /locus_tag="KQD82_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG4379739.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            13310..13903
                     /locus_tag="KQD82_001262"
     CDS             13310..13903
                     /locus_tag="KQD82_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG4379740.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            14085..15431
                     /locus_tag="KQD82_001263"
     CDS             14085..15431
                     /locus_tag="KQD82_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG4379741.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            15613..15984
                     /locus_tag="KQD82_001264"
     CDS             15613..15984
                     /locus_tag="KQD82_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG4379742.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            15977..16666
                     /locus_tag="KQD82_001265"
     CDS             15977..16666
                     /locus_tag="KQD82_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG4379743.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            17186..18052
                     /locus_tag="KQD82_001266"
     CDS             17186..18052
                     /locus_tag="KQD82_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4379744.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            18294..19103
                     /locus_tag="KQD82_001267"
     CDS             18294..19103
                     /locus_tag="KQD82_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379745.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            19247..20311
                     /locus_tag="KQD82_001268"
     CDS             19247..20311
                     /locus_tag="KQD82_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG4379746.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSIKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            20339..21058
                     /locus_tag="KQD82_001269"
     CDS             20339..21058
                     /locus_tag="KQD82_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4379747.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            21082..21666
                     /locus_tag="KQD82_001270"
     CDS             21082..21666
                     /locus_tag="KQD82_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG4379748.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            21668..23017
                     /gene="pabB"
                     /locus_tag="KQD82_001271"
     CDS             21668..23017
                     /gene="pabB"
                     /locus_tag="KQD82_001271"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG4379749.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTSIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            23007..23750
                     /locus_tag="KQD82_001272"
     CDS             23007..23750
                     /locus_tag="KQD82_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBG4379750.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYTKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDVPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            23784..24485
                     /locus_tag="KQD82_001273"
     CDS             23784..24485
                     /locus_tag="KQD82_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379751.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            24511..25083
                     /gene="folE"
                     /locus_tag="KQD82_001274"
     CDS             24511..25083
                     /gene="folE"
                     /locus_tag="KQD82_001274"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG4379752.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            25147..25938
                     /gene="folP"
                     /locus_tag="KQD82_001275"
     CDS             25147..25938
                     /gene="folP"
                     /locus_tag="KQD82_001275"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG4379753.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSYGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            25949..26314
                     /gene="folB"
                     /locus_tag="KQD82_001276"
     CDS             25949..26314
                     /gene="folB"
                     /locus_tag="KQD82_001276"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG4379754.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVRVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            26307..26813
                     /gene="folK"
                     /locus_tag="KQD82_001277"
     CDS             26307..26813
                     /gene="folK"
                     /locus_tag="KQD82_001277"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG4379755.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            26794..27579
                     /gene="aroF"
                     /locus_tag="KQD82_001278"
     CDS             26794..27579
                     /gene="aroF"
                     /locus_tag="KQD82_001278"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG4379756.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            27834..29624
                     /locus_tag="KQD82_001279"
     CDS             27834..29624
                     /locus_tag="KQD82_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG4379757.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            29642..30808
                     /gene="rpoD"
                     /locus_tag="KQD82_001280"
     CDS             29642..30808
                     /gene="rpoD"
                     /locus_tag="KQD82_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG4379758.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            30973..31662
                     /locus_tag="KQD82_001281"
     CDS             30973..31662
                     /locus_tag="KQD82_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG4379759.1"
                     /translation="MKLTDRLLKIASLASDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            31676..32773
                     /locus_tag="KQD82_001282"
     CDS             31676..32773
                     /locus_tag="KQD82_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG4379760.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            32836..33408
                     /locus_tag="KQD82_001283"
     CDS             32836..33408
                     /locus_tag="KQD82_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG4379761.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKMGIRDYLKRLNI"
     gene            complement(33468..33935)
                     /locus_tag="KQD82_001284"
     CDS             complement(33468..33935)
                     /locus_tag="KQD82_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG4379762.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(34321..34797)
                     /locus_tag="KQD82_001285"
     CDS             complement(34321..34797)
                     /locus_tag="KQD82_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG4379763.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(34917..36008)
                     /locus_tag="KQD82_001286"
     CDS             complement(34917..36008)
                     /locus_tag="KQD82_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG4379764.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKLEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(36001..36390)
                     /locus_tag="KQD82_001287"
     CDS             complement(36001..36390)
                     /locus_tag="KQD82_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4379765.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            36582..37028
                     /locus_tag="KQD82_001288"
     CDS             36582..37028
                     /locus_tag="KQD82_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379766.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            37292..38017
                     /locus_tag="KQD82_001289"
     CDS             37292..38017
                     /locus_tag="KQD82_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379767.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            38004..39248
                     /locus_tag="KQD82_001290"
     CDS             38004..39248
                     /locus_tag="KQD82_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379768.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            39328..40005
                     /locus_tag="KQD82_001291"
     CDS             39328..40005
                     /locus_tag="KQD82_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379769.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            40006..>42537
                     /locus_tag="KQD82_001292"
     CDS             40006..>42537
                     /locus_tag="KQD82_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4379770.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEHKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENI"
BASE COUNT        17292 a         4308 c         7228 g        13709 t
ORIGIN      
        1 tattatgtat ataaaaatga tagaaagcat ctaattttaa atgattaaag agcaagctta
       61 aataaatcaa acttgctctt ttaacatagt gtatgttttt atttgtgata gttagataaa
      121 aatttatctg tgttttttac atctgcaaaa ctgttctgta gtgatgccat gaatacttga
      181 tgtacagtat ttgcattaat ttctatatta ttccaagata aattcttcgt tgtacatgca
      241 tcgcttatta gagttatgtc gtatcgaagt ttctttgctg ttcttacact tgtatcaata
      301 cacatatggc tcatcattcc acagattact agattagtaa tatttttttt atcaaggata
      361 ctttgcaaat ctgtttcaaa gaaactatct ggtgtgtgtt taattataat ttcttcatta
      421 tctagtggaa aaacaatttt atttatttct gaaccatgag tattaggaag aaaaaaagaa
      481 gctgttttat ctgttgatat atgctgtata tgaataactg gtaaattgtt ttttctaaaa
      541 aacagtagta tttttttagc attttcagca gtttcttctg attgaaataa ttcacatttt
      601 ccatttttaa aataatcatt ttgaatatca atcaatatta aagctataga catgattata
      661 cctcctattt atttttatta taatactatt gtaaaaaatg taaattactt actttttggt
      721 atgtatagga ttacttattg tagcaaattc ctttattatc tagaatttct tgttgaccat
      781 gttcaagcat atattcgatt cctatttctt gcattatttc cactgctttt aacatttttt
      841 taccacgatt agtaagaaaa tattcaacat gtaatggata tccagcagaa cttattttat
      901 caataatccc aaaatcacgt aactctttta attgttgaat caacattttt tgatttattc
      961 cataaatatt atgttgcaat tcagaaaatg ttttattaga ttgtcttaat tcaaatataa
     1021 ggatagtttt ccattttcct ttgattatgt catgtacaat ttcaagagga catgtatatt
     1081 cagctcttat tttcatagca tatcaccccc agtaaaatga taataaatta tatcacttgt
     1141 cttgtgatat gacaaatata tatgaactgt atataaattt tatgttttat taagttctaa
     1201 atttcttcta ttgtagttca tatttagtac ataaataatt agtaagatat aagtaaataa
     1261 atatataaca tgactataga aatttggata aaaataactt atgataattt agaggaggaa
     1321 tatatatgaa tatatcaaga aaagctatga aaataataga attagcacaa aaaattgcaa
     1381 ataagagggg gatttcagta gaagaagctt ggagtgaagc agtaacagaa tataaaaata
     1441 aatacgaaca tattgcatag atatttaaaa atttagaata tttggtagaa tataatgatt
     1501 ttaaatgata ctataaatta taaggtatat cattattaac atagaaggag gatatctgct
     1561 tgattgtaaa aatagataat actttagaaa aagaattttg gcaatatgta tcacatgaag
     1621 aaagtctaaa tttattcata attggatacg ttgagaatta tggatttagc tctcaatatc
     1681 aagatatatg gtctcaggta gaagatggaa atataacctc tataatatta aaaaataagt
     1741 caacacttat aatttatagt tttaaaaata attttaatat aggagaaatg aagaatcata
     1801 taaaagattt agatgtagaa agcataagtg gaaaaaaatg tgtaatagat agactaataa
     1861 gtaaatacaa agatttttat gaaaaactag ataataaatt ttgtgtatta aaagaaataa
     1921 aagaaataga tttttccaac atgaaggagt ataaaataga aaatgctcag gaaaaagata
     1981 ttgatgaaat aggaaaactt ttaaataggt cagactataa agtaagtaaa aattacatag
     2041 aagaaaggaa agaacattta aaagagggaa atgtaagagc atattttatt agaaatgatg
     2101 atactatgat tagcactgtg tcaactggta tggaaactag ttttttggca atggtggtct
     2161 ctgtaagtac agataaaaga tataggggaa aaggacttgc aagttatatg gtttacaatt
     2221 tgagtaaaga attattgttg gaaggaaaag ttccatgtct tttttataat aatgatgtag
     2281 caggtaaaat atatcataat ataggataca aagagataaa tgaatggaca attcttttta
     2341 aatagctata aaaaataaaa aactctatcg agaattaaag tatttataaa taactatatt
     2401 tttactataa ttcatgatag agttttttta aatttatgat actatttttt attatttaaa
     2461 taatcaagtg ttttaagttt ataaccttct ttttcccaat gtgtaagaac ctcatctaat
     2521 atctcagtat ttgttgatga atttggatgc agtaaaacta ttgcaccagg atgagttctt
     2581 gaataaattt tgtttttggc aaattcatga gtaggttgtt tcttttcata ccaatctaca
     2641 taagcaaaac tccaaaatat tgatttatat cctaaatctt gtgtatattt taaagattgt
     2701 tcgctaaatt tacccattgg aggtctaaaa tacttaggca tttcttttcc agtaacatct
     2761 ttgtatgctt tttctacact tgtaatttct tctttgaatt tttcaaaatc tgtaatacct
     2821 gccattgatg gatggctttt tgaatgatta caaactaaat gtccttcttt ttccatacgc
     2881 tttattaact ctggattgct ttttatataa ctttcaacta cgaaaaattg agctttagca
     2941 ttgtgttttt taagcgtatc taataatttt ggagtgtttc cactttcata tccagcatca
     3001 aatgatagat atataacttt ttcattggga tttcctacat aataagctcc gtatttttta
     3061 aaaaaatcag cttcttttat aggtgatggc tgtttaccat cctccctagg gttaaaatac
     3121 caatcatatt catgtgtgtc gagtgttgga ctagaaattt gagtttttgt tttatctaat
     3181 gatttaaaat ttatactagc aattccaaaa agtactagag caaatgctcc tatcataatg
     3241 tattttttta ggctatcttt tttcacaatt tcacctctta tttaaaattt aaaatatatt
     3301 tcttataata tagtttgaat atttatagtt aaaaatattc acatatacaa ataataaatg
     3361 aaagaagttt actgtaagat ataaagttga tattaattat cttaaataaa gtgtatataa
     3421 atgataaaat ataaagagaa atgattgtaa ataagttagg ctggtttaac ttgaagggat
     3481 ataaaaatgg ataatataat aagtgtattg aatgatgttg tttttttaga tatagaagtt
     3541 agtggattag attgtttaaa ttcagaaatt ttagaagttg gagctgtcaa ggttaaagac
     3601 tggaaaatac atacctatga atcattaata aaaaacaaat ttgaggtacc tatagaagta
     3661 ttctcagtat gtaaaaattt agataaaaat gatttagaaa tagctaatga aatagaactg
     3721 gtggaagata gacttgtaaa ttttgtagaa gatagtttca ttatatgtca tgatttaagt
     3781 ctgaaaaaaa agttttttga gtatcatatg ccaaagttaa aaaacaaatt tattgattta
     3841 attgaattag cagttatttt agaaccatat cataaagatt atagcttaga atacttaaaa
     3901 aatactttaa caaattgtaa ttctaaagtg gaaaatagag ctttatcaga tgctattgat
     3961 ataataaata tagtaaattg ccttttagta aaatttaata actatgaaaa aactactttg
     4021 gagccattga gttttaaaat aaattcatat cttaaaaaat ttaatcttcc aacatgggaa
     4081 tggagcaaat ttttagaaga ggcaaattat gatttatcaa ataatataaa tataaaaaaa
     4141 gaatataata tatttgactc aaaagaagaa aagaagaagg aaagagaaac cttaaaaatt
     4201 ttaaatgaag aagaaaaaaa ttatgaagaa ttattaaaat acaaaactat ttgggagaat
     4261 aaagaaggat ttacatatga atatagacct ggtcaatatg agcttactaa aacaataaga
     4321 gagttgttta gaaatagcga agatgaagaa aaaattgcat gtatagaggc accaactggt
     4381 ataggtaaaa gtgtaggata tttattgcct gcaatattag aggctagaat taataaaaaa
     4441 agattattaa tttctactga cacaaaggaa ttgcagatac aattaataaa taaagatatt
     4501 ccaaatgtgt tagattcttt gggtctaaat ggaaaagtaa gttacggata tataaaggga
     4561 aaaaataact atatttgtat agatagactg gaagcatata tagatgatta tgagtctcaa
     4621 aaccctacta aaggagaaat tttatcttta atatttttga agagattagt tgaaggtggc
     4681 aagtatggag atatagaaga aataaattat ttagtgtttg acaacttcaa ggaaataagt
     4741 actcacttaa gaaatgtaag ttgtgaccca aatatgtgca gacctaaaaa atgcaaaaaa
     4801 gattgtttat ataaaaacag aatagaagaa ttaaaagaag aacatataac agtagtaaat
     4861 cattcactat tagctaaatg gccgtataag gatgaaaaac ctttagaaaa tataatagta
     4921 gatgaagcac ataatttgac tgaaaaagga tatgatttct tttcaagcat tataaattca
     4981 aagtctttaa ggtatttatt acaagaaatt tatccttacg agtttattca aaatagtagc
     5041 tttatatata aaaaatatag tagaaatatg agaaaaataa aggcttttga taaattttat
     5101 aatgtattaa aaattggaag agaagataaa caaaaaattg ctagaagcat aaatctcatt
     5161 attgaagaga tagattcaat attaaatttt ggtaattgta atgaatataa taatgtaagt
     5221 aattataatt taagatggga actgaatttg caaatagatg aaattgtggg aaaactaaaa
     5281 aagggtggta tagatactga aataagttat agagcctata gtgaaaagat aaagttgagt
     5341 tgtgagaaaa taataaaaaa tttagtttca ataataataa taatttatag gaatattgat
     5401 gatgatagta tagataaaga agcagatata tataaatttg gaaaggcaaa gacaagagat
     5461 ttagaggata taaagattat atttgaaatc tttttagagt atgatgaaaa ggatgattat
     5521 gcaaggatag ttgaaattga taagaattat aatgactttg aatttcgagt agtgccatta
     5581 aaaatagcag atttatttga agaaaatatt ttatctcaat tagaaaaagg aatattttta
     5641 tcagcaactt tgagtttatc tgaaagtatg tcttatttta aaaatacatt aggaatagac
     5701 agagttaaga atgtggaaaa gattatagaa ccaatttttg attataaaaa tagagtctct
     5761 gtagttggat tttctgatat ttgtgagtac agaaattctg aatttccaaa tgagatgagt
     5821 aaaattatat ctaatatatc aaagattaca gaaggtcata cattagctct ttttaatagt
     5881 aaagatagac aagaaaaaac ttatgaaata ttgaaaaaat acttacatag ttttaactta
     5941 gaaatttatg ctgataaaaa gggcattaga caccttaatg atttaaatag aaaatgtgta
     6001 gtacttggct caaaaggatg ttttgaaggc gtagacattc caggggatgg tttggtttgt
     6061 gtaactttag acaagcttcc gaatctaaat ccaaaagacc ctttgtattt tactataatg
     6121 aagaagtatg gtatagacta ctatactata aattatcctc aaatgaccat aaaggtaaag
     6181 caagctatgg ggagaatttt aagaagcaaa tatgactatg gatgttttgt gatttttgat
     6241 gtaggaacta atataagtgt tttaaagaga ttagagaaag atttacacga ttgtaaaatt
     6301 tcaaaggtta atagcaatga gttttatact tatataaggc gacatttaaa taaatctaga
     6361 agtttaatac taaaatctgt tatatttgat actataaaag cgttgaatgt tgatgctaaa
     6421 atggataaca atgatgttga taaggatatt ataaaaaaag atataaatga aaatataaga
     6481 caaagagctg taaaaggaga agtgtaccat attgatatta taaaaaaaga tatgaaagta
     6541 aaatattttg atagaaacta tcttataaat ttagatatat ttatgagaga agaagataaa
     6601 aattaagagt gggagtgata tatttggcaa aacaaaagtt ttatgctgtg aaaaaaggta
     6661 aaaatatagg tgtatataat acatgggatg agtgcaaaaa acaagtaaat ggattctcag
     6721 gagctgaata taagagtttt tctacttttc aagaagctaa agaatatatt gatgggtcag
     6781 aaaaattgag ttttcaagaa gataaggagt ttatagaagc atatgtagat ggaagttatg
     6841 aacattcagt aaaaatgtat ggttcgggtg ttgtgatttt aaaaaataat gaagttataa
     6901 aaacttatag tgaaaaagga aaagagaaaa ctttagtaag tatgagaaat gtagctggag
     6961 agatagaggc ttctaaaatt gcaatgcagt actgtattga caataatgtt caaaacttga
     7021 ttttatactt tgactatgaa gggattgaaa agtggtgtac tggagtttgg aaaacaaata
     7081 aagaaggaac tatagcatat aaaaatttct atgacagtat taagaataag cttaatgtaa
     7141 aatttacaaa ggttaaagct cattctggaa ataagtataa tgaggaagct gataaattag
     7201 ctaaaaaagc tattggtgta taataaaaaa atatttatac ataattacat ggtataagag
     7261 tatatctaaa ttcctacttt ataattaaac aacttgtaat taaacaactt ataattaaat
     7321 aaattttgat taaaaaagca gattgtatta tgaaaaaata agtatctaat ttataaacaa
     7381 ttaataatag tattaattag atacaactta tttaatataa taaaatctgc tttttatata
     7441 actataaaat tttcctacat tctattagaa ttgcccataa ataccttcaa gttcttcttt
     7501 taatacttca gggtcattta taccaataga catggttcta gccagtatat ttgcttggtc
     7561 ataagttgat gtccatgaga atacaccacc tagacaattt ctaagaacat attgagtttt
     7621 taagaatatt gataaagcat catcatatga cattgcaaaa tctccatcct taactaaata
     7681 tggaacttga gcagtattat caaatctata ttcatatcca tttttattta tataatccct
     7741 tctaagctca tcataagttc ttgttatagt agcacctaat cttccataaa atggtatacc
     7801 aagaaggatt ttttcagaag gcattccagc attctcaaga tttcttacca ttgcatcaac
     7861 actatatcct ggcaaagata agtctgaatc aaaaagattt gcttgatgtt ttctaccatt
     7921 tgggcctgtt tcacctgctg taaaatcata actcataaga ttaaaataat ctattatagg
     7981 agctatttta tctatttcag cacttgaatt tatatatcct ctatctcctg ttccagctac
     8041 actaagccat ttatcatctc ctataacatc tcttatggca gttagtaaga gtgtaaagtt
     8101 ttctctatct tgaggtcttg atgttattcc agatgcacta cttccaggat attcccagtc
     8161 tatatctatt ccatctaaag catattcatt tatcatctga ttgacctgtc ttgcaaaatt
     8221 atatctagat gtaggtgtta atgctgcatc agagaatcct tcagctcccc aacctccaat
     8281 agctacaatt acttttaagc taggtttttc acctttaaga gcaagtaatt gatttaaaaa
     8341 tctaggataa ggaataaaca aatttccaga ctggtctatc tcagcaaaag caaatataac
     8401 tgcatctaat agttgagcat ctacattagt tggatttcca agaacatatt ctgctacaat
     8461 tggatttata tctggacagt ttgcattttc aactattgga ttgtatgggt ctgttatttg
     8521 tggtctaact aatttttttg aatcttttaa agttttcata gctatttttt tagtattttt
     8581 ttcgctagta taatcgtagt ttttataatt atgtttatag aaatcttcat aactactatc
     8641 ttctttatca tagctagaat ttattttgtc gcaagattca tctttatcat agttaaaatt
     8701 catttcatca caagacttat ctttaaactt atcttgtttg gagttgtgaa ctttgttagt
     8761 atttttagta tgttttttct catggttgca atcatcttta atatcagtct tgtgttcttg
     8821 tttacaatca agagctttgt tcaggtagtc atgatgttca tgttcacagt tacattcatt
     8881 ttcaatgttt ttagtatgtt cttttttcca gctacaagta ttatcaattt tcttgctcac
     8941 ttcttcatca ttataattat ctggtacaaa acataagtac cagtccatac atgaatattt
     9001 cttattacaa ctgttaactc tattttttaa ttccttatag tttttaggat aaggtaaaat
     9061 cactggcatt ccaggaaacc agtttgcagg agtaactata ttatctctat cactagtctg
     9121 aagtgcatct actattctaa gtatttctgg aatatttctt cctgtagtta gtggataata
     9181 aagaatcgtt cttagaattt gattattatc tataataaat acagagcgaa cagttgatgt
     9241 atcactcatt ggttttgata tcatgccgta taacttggca attctcatat ctctatcttc
     9301 tataatagga aatggaattt ctacacctgt aagtaaagaa atattataca tccaagctaa
     9361 atgtgaactg ttactatcaa cacttagacc aattagttct gtatttcttt ttttaaattc
     9421 gtcataatat ttagcaaaac ataaaaattc tgtagtacaa actggtgtaa aatcaccagg
     9481 atgtgaaaat aaaacaatcc aattaccctt atagtcagag agtctaatag gaccatttgt
     9541 tgtattggct ttaaaatcag gagcctttga ccctaaactt ggcaaatttg gcatgtaaat
     9601 cacccctcaa taaattatca tttattttat tctattaatt aatacatatt agtgttacta
     9661 ttttattaaa agttaagtaa tccttatgtc aaaaaaagat atttattaaa aagatttaat
     9721 attttcctaa tataaatgac aatagtaatt gctatttttt tactaatttg gtatagtaat
     9781 ggataagggg tagtgtcaat tttagtgatt atacgaggtg agtggattga aaaaaattct
     9841 aagaatttta gttatctcca tgatgatatg ttttgcaatg acttttgcat tagtgttaat
     9901 aaattcagtt atgggagtac atataggatt ggattattac tttttgatgg gaagtgtgct
     9961 tacagtttgt ttaacaattg gaattgtttt atgcaatgct agaaaaatat ttgatagcaa
    10021 agctatacac aagacaagaa cgaaaactgt cagtaataat aaaaaacaaa agagtcattc
    10081 tagggatata aaaagaaaga tatcataatt tatgtaaagg aaatatttat gtataaaata
    10141 atgattgata ggtaaaataa aaacacgatt taacttgtgt ttttattttt tttcaagaat
    10201 tatgatataa taaccaataa gaaatttagt ttaccataaa atataaagat tattaaatgg
    10261 agagggaact ataatgaatt ttagagattt atttgagaag gctgtagatt ttattgatga
    10321 aaaaaggaaa tctatagttg cgatttcatt atcaacatta ggtgtaattg ctttgataat
    10381 agtatttttc ttaagtagta acgagtttag tgtaggaaat gaagctaatg agttgttaaa
    10441 gattatagag aaaagacagt attctatagc tgttgattac tacacaagta tagaaaaaaa
    10501 gttttctgat tcaaaaatgg aaaggtttaa taagtctgtt tctaaaaaaa taaataaatt
    10561 gctattaaat agtggtgata aatatttaga tggagacata agtcaagaaa gttttattgg
    10621 attaataaat accgtgaaag aattaaacaa aataagtata gatgtggatg acttattagc
    10681 tcaagctgat agagttagag aaatgtataa agaagaaaat actacttatg atattgccat
    10741 taactacata agtactgttt caatattaaa tggtatgggt agtaacctag atgtatacaa
    10801 acagaatata gaaacaatca aagagtctag agatgtttat gactcagcag taaaagacca
    10861 aaaagcatat aagtattatg aagctattga aaagtataat aaagtattaa aagaagatga
    10921 aaagtattac agtatggctc aaaatgggaa agaacaatgt attgaagaaa tgtatgatta
    10981 ctatataagt agagctgaag aggcaaatac ttctggagat tatgaaagag cattacaata
    11041 tattgagtat ttaaaagacg attattcaga tgatgaaaag gtacagagtt tagaaaaaaa
    11101 atataaaaag aatttatcat tgtatacttt aacatctgat gacattataa atgtaatagc
    11161 taaaaaaagt ggcaaagaca aggcaaatct atctataaat tcacttccac aaatgataaa
    11221 aaataataaa tactattatg ctgaagtata tgagtatgat aaattaataa atgaagtttt
    11281 ggtaagtgca aaaactaaag atttatattc atataaagat ggtaaaaaag attacaaagt
    11341 agattatggt gatggatact ttagaatatt agaagatgga agttatcaat ttggcattac
    11401 aaaagataaa gctaagtttg tgttaactaa tactctcgat gaaaaagaaa ataagtataa
    11461 aaagattgag atattagata tagagaaagc agatagatat gtaaaaagta aaaagagttt
    11521 agaagaattg tttggaaaat ataaaaatat ttattattat gctgtagtaa ataaaggctt
    11581 atttagaggc aaagaagttt atgctattaa tatttacaat gaaaaaatat atgccatatc
    11641 agaaaatgga ctaaatgaat attaataata ttaaattaat atataattaa aaagtttttg
    11701 gggttttaag gcgcattatg tagtaatata ataataggga tatataaata attttattgt
    11761 aaggagaagt atgggatgag taatgtgaac aaaaagctag ttatagctat tggagctgta
    11821 gcattagtag taataatttt tataggcata atgtgcatgc aatttaaagg ggagaaaata
    11881 gcaaaaaaca catatgttaa tggagtaaat attggaaaat taactaaatc acaagcaaaa
    11941 caggaattag ctaaaaaata caaattagag aatgttgatt ttaattacaa tgataaaagt
    12001 tggaaagtaa agagcaaaga tttaaattta tctactgacc tggataaaac agtagaaaac
    12061 gcatataatt taaatagaaa gagtgctttc tttggcaatc tatcaaaaac tatttcagca
    12121 aattttggca aaaaaagcaa cttagtagtt gtgataaatt atgataaaaa taaattaaaa
    12181 gcagaaatgg aaaagatagc taaagaaata gatgtagatg taaaagatgc tacacttgat
    12241 ataagtggtg aaaaagtcaa ggttatacca gattctgatg gtcttaaaat ggatatttct
    12301 aagagtatgg agaattttga taatcaaact aaaaaaggta actataaaaa tgagttagta
    12361 gtaaaagcta ctccagcaaa agtaaaaaaa gagcaacttg ctaatataga tacaaattta
    12421 ggtacgtatt caacaacatt taaaacttct caaataaata gaagtataaa cataaaatta
    12481 gcaacagata atataagtaa tgtattactt atgccaggag aaactttctc atttaataaa
    12541 catacaggga aaagaagtaa ggaaaatgga tataaaagtg cacctgtaat aatggaaggt
    12601 gagatggaag aagattatgg tggaggagtt tgtcaagttt catctacgtt atacaattct
    12661 gtgttatacg caggtttaga aattgttaat gtaaaaaatc atactatacc gtcaagttat
    12721 gttccaaaag gaagagatgc aactgttgca gatagtggaa tagatttctt atttaaaaat
    12781 aatctaaagc atccagttta cataaaaaat tacgtttcag gaaatcaaat tgtttgcaat
    12841 atatatggaa gtgcagaaga taaacaaaat ataacaatat caacaaaatt agatggcgtt
    12901 tcacaaacta caatgaaaag agttaatgac cctacaatgc ctaaaggtaa agaaaaagta
    12961 gacaaaagtg gaagaaatgc atattctgta tcaacatata gaacatttaa tgatgcaaat
    13021 ggtaaaaaaa ttaaaactga aaaaatagct aattcatatt atcctaagaa ggaaggtatt
    13081 atattagttg ggactatgga accaaaacca gaggaaaaac caaatacaga cgaaaataag
    13141 aataatcaaa atacaaacaa tcaaaatcca aataatcaac aaaaaccaga aacaccacca
    13201 actgataata agcctaatga gacacaacca caaccgcaag cttaaagttc tcaagaatag
    13261 gatttatgta gagtaataaa aaatataaat ctaaatagga gggattactt tgaatttaaa
    13321 taatataacg aattacgttc aaagtatttg cgagaatatt tctaatgtct tggatgtaga
    13381 tgttacttta gttacaaaag atttaactag gatagcaggt acaggaatat tcagaggtaa
    13441 aataggagag acaatatcag agaagtcagt gtactctctt gttttaaaag atgggaaatc
    13501 ctatgttata aataaagaaa tagaagagaa ctgtaataaa tgtgcaaata gagaaaattg
    13561 taaagagtta gctgatatat gtactcccgt taaaatggga gagcatatac ttggaatctt
    13621 gggtatagct gcgtttaatg aagaacaaaa agaaaagata ctttcaaaag ataaggagtt
    13681 aactgctttt gtaaggagta tgtctgattt aatatctttt aaactagatg aactttataa
    13741 acaagaagtt aagactatag atgaccttga gagggaagct atagaaaatg ccataaagaa
    13801 atatggaagt aatacagaag gtatgaaaaa agtagctgag gcattaaata taggtatagc
    13861 tacactctat agaaaagtaa aaaaattcaa cattaaaagt taatagtatt gaaaaagagg
    13921 taaccttttt atgaaaagaa gaggattacc ttttttatta tgagaatggt ggaaaaatat
    13981 gtatagtttt gacttgacat attaaatatg ttatcttaaa tatatatttt agagtggagg
    14041 tgagtattat tttagttaaa gatataaaac aagacataga aagcatgcct agaaaaattt
    14101 tagatatatc tttgatagaa gataataaaa gtgatttgac tttttttaat atagaagaag
    14161 aagacggtga tgaaacatta aaagcattaa aacagagtta tgtgactaca aattataaag
    14221 aagaaactct attaaacttg caaggagctt taaattcaaa gtcagataca acgtctaagg
    14281 agttaaaatt agaatcatat aaaagatata acaatgcatt ggatttagca tataaaaatt
    14341 atataactag tgccattaat atggttaata aagctctgga gataaatcct aaagacattg
    14401 atatactaaa tcttagaggt cttttaaaat tattaaaatg tgattttgct aaatcatttg
    14461 agagttttta tactgcttta tgttatgaaa ataatcatat atctagaaaa tatgtaaatc
    14521 taatttcatc tgaagatttt aaaacttttt taggaagata caatcattct ataagattta
    14581 taaatgaaga attaaattat gagtcaatac atattttaga gaacataatt gaagaagaac
    14641 ctgaactaat agagccatat gtaattttat ctttgttata tgataaacta ggtaatgtaa
    14701 aaaagagaga aggatattta gacaaactta aagagctaga taaagataat cctgtatttg
    14761 aaaaaaatga agaagaaaaa gaagatacaa gtaaaaagga agaaataaaa aaagtaaaaa
    14821 gaaagaaaag tattttgcca tatattatag ttggctgtgt tttaataggt atggggatat
    14881 atttgattca aagcaaaaag agaatagaaa acttgaataa ccaaataagt agtaaagagg
    14941 aaaagttaag tgaaactgat aaaaagttag gagaaacgag taaaaaatta gaaaaaacga
    15001 ataaggaatt aaatgaggtt aaaaataaag aaccagaaag agagataact gtagctgatg
    15061 aggaagattt atattatcaa gctctaaatt taaagaaaaa caaagaatat gaaaaagcca
    15121 tagataattt taaaagtgta gtttcttctg gtaaaactaa aaaatatatt tctgagtcaa
    15181 tatatcaatt agcaataaca aataaattat taggaaataa ggatgaagct ataaaatatt
    15241 acaaaaaata tataaatact tatactaaaa atgaccagta ttatgatgat tcatattacg
    15301 agttgggtat gctttattat gataatggcg atttaaaaaa tgcacaacag actttttata
    15361 gtttgagaag tgaagttcca gacagtatgt ataacaattc aaaaataaaa gaaattttaa
    15421 gtgaaaaata ggtttatata gttaagtttt ttgaaacaac caagtatgta ttttattaaa
    15481 ttaaaataaa aataaattta aaaagagatt gtgtcaaatt ataactttct attttaaaga
    15541 aagatatttg cttgttgata aaactacaaa tatatgattt gaagcaatct ctttaatttt
    15601 tggaggtgga aaatgaaagt tttcaatcaa ttagtaataa ttttatctat ctgggtaata
    15661 ggagagtata taagttcctt tatacaagga attatactta ttcctgggag tatagttggt
    15721 atgctattat tatttctact actacaattt aaagttttag acttgagtag tatagaaaat
    15781 gtaagtggat ttttcctcga taatatggct atatttttta ttccagcagg tgtatcactt
    15841 ataaagtcat tagacctaat aagggaaaat gtattagtgc ttttattagt catatgttta
    15901 agtacactaa tagtaatgta tactactgga attattgtgg agaaaatgat aaaaaagaag
    15961 gaagaaggac aaaaaaatgt ataatgtatt acagacacca gtatttggta taatagtaac
    16021 aatagttttt ttcaatttag gaagatacat tcaacgaaaa acaagtaatc ctatatgtaa
    16081 ccctttatta attgcaatcg taggaataat attgttttta agcatatcta aaatccctta
    16141 tgagaattac aagataggag ctgatagtct aaatttcttt ttaggtcctg tcacagtagt
    16201 acttgcagtt ccactttata aacaatttga attatttaaa aagcatatgt ttgagattat
    16261 tgtaggaata ggttgtggta tagtgatttc ttttgtatct gtattgataa ttggcaaaat
    16321 agctaattca gatgtaagta taataaattc actaattcca aaatctataa caacaccaat
    16381 gggaatatct ttagcaaatt ctttaaatgg tatagaatca ataacagttg tagccataat
    16441 ttttacaggt atttttggtg gtatggttgc ttctacagta ttcaaactgg ggaatataaa
    16501 tcatccagtt gctaaaggaa ttgctcttgg aacatctgca catgctttag gaactacaaa
    16561 ggcttttgaa cttggagaaa tagaaggagc tatgagtggt gtttctattg gagtctctgg
    16621 tacaattaca gtaatactaa ttccaattat aatgaatttt atataaaaaa taaaattaag
    16681 acgagtacct tgaatactta aaacaaaata aaataaaaaa aatttaaaat aaaaactgaa
    16741 cgaaaaaaat gcaaataatg aaataaaaaa atcatgtact gaaattataa aaagaaaaat
    16801 ttcatttagg aattaattat aaagagacaa ttactaaact tgaatttgct aaaataaaat
    16861 aaattactgc gaaaatagat atttagaaaa aaatactata aatttactat attgcaagtt
    16921 aaataataag cagtatcata ttatgctaga aaaagtttca tttaaggcat aaatgagatg
    16981 atttgaacgt ttttaaagat tagaatataa aagattgcac tagacatata tgaaaaagat
    17041 aatataatag atgatgtaat agatgacagt tcaagaaatt ataaaaaaaa tttatttgaa
    17101 atattatcta ttacatcagg aataatatat gtaagagtaa gcttttaaaa ttctaaagat
    17161 ttagaaagat tgaagggaga aagaaatgag tgaagttaag aataccctac aaaaacatcc
    17221 agttttaaga tattcaacaa tgctatttgg gatgttgatg acatctatag gaataaatgg
    17281 gttcttaaga ccagcacatc tacttagtgg aggtgctaca ggtatagcaa catctataaa
    17341 ctatttgaca aatataaatg taggtttatt aacattttta atcaatattc caatatttat
    17401 cttagggttc atataccttg aaaaagagtt ttgtatttca agtttggtaa atatgattgt
    17461 attttcatta ttattaggag caactcagga aataagtaat attataccta tacatgatat
    17521 attgcttcaa agtgtatatg gtggtatatt atctggttta ggagtcggag ttgtatttag
    17581 aactaggtct tctcaaggtg gtacagatat aatagctgca atactaaaaa ttaagaagaa
    17641 tatagaaatg aaagacactg ctttagcaat aaatggcttg atagtactta caggaagttt
    17701 tttatttggg ttggatttag cattatacac tcttataggt ctatttctaa atgcgtattc
    17761 aatgagtttt attaaagatg caatgaatta ccaaaaatct gttatggtaa tgtcaaatga
    17821 agtcgattta atagcagaag atataatgaa gagtttagtt agaggtgtaa cttttttaga
    17881 tgcagaaggt gcatacactc atcaaaaaaa gaagattata tatacaatag tttcatcaaa
    17941 tgagatacca aagataaaag atatagcttt gaagtatgat aaaaaggcct ttatatctgt
    18001 aaatgatgtt acagaagtaa agggaagagg gtttaaagca aaagatttat agaaaatttt
    18061 acaagctggg agtttataat gtaaatttgt atattatatc tctcagctta tttaaattta
    18121 gtatacgaaa ataaaggttt tattaatgat ttgtgtatca ctttaaaaga tatcatttta
    18181 aaattaaaat tgaaaaaatt acttaatatg tagaaaacat gaccactatt aggatataat
    18241 aaatttaata gatttagtaa aatatctagg aataacgttg gaggaatggc attatgaatt
    18301 tatctaaaga atatgttaat ttgtatgata attatttgaa atatataaat gaagtaaaca
    18361 aagagattag aagtataaag aacatgaaga aacgtgtttt aaaaaggggc gattttgctc
    18421 cagaaaatga agtattagaa ttggaaggaa aaacaatacg cgatatagat gatgtcgata
    18481 caaaaaaacc aaaaaataaa atctataaat tttcaaatgg ggatgtatat gtagggaaat
    18541 tcttagaggg aaagatggag gggaaaggct catatacttt ttttgtagat gaaggaacag
    18601 ttatggagta cataggagaa tttaaagaag ataaaaaaaa tggaaaagga aactttatat
    18661 tttcaaatgg aaatgaatat ataggttatt ttgaggaaga tatgatgagc ggtattggaa
    18721 atatgttata tagtagtaaa gatgaatata ttggaacttg gaaaaacgga aaaaaagatg
    18781 gaaaaggtat ttataagtgg agtgatggat gtatatatgc aggtgaattt aaaggtggaa
    18841 agatggaagg gtacggtatt tgctataata gtaaagggga agtactttat gaaggagaat
    18901 ggaagaataa tttgattcat ggtaagggaa cttatatatg ggaaaaaggt aaaaagtata
    18961 taggtgaatt tatgcatggt aaaaaacatg ggcaaggaac tttttatcta aataatgaat
    19021 tggtttatga agggacttgg aaatttgata agccatctat atttgataga acattagatg
    19081 aaatattttc cttgagactg tagctttatc taattagtag ataaagcttt tttctaatac
    19141 ttactgtaaa taggtatgaa tgatattgat tagtatacac tatcaatatt aacttaataa
    19201 atgcaatatt aaaaaatttt gattgatttt aagaaggaga gatatattga agagattaat
    19261 tgctttagtg ttagtagtac ttattgtttt attttatagt cctgaattat taaaagaagg
    19321 atatgattta aaagaaaagt tttttcaaag tgaaatatgg aatgaatttt tagacaaaat
    19381 aaatataaat gataattcta agaaagataa aaaagaaaaa agtcaaaatt tacaatcaaa
    19441 taatactgaa ggggagaact taggagagtc agatataaag aattttgaaa aaataagctt
    19501 aggagataat ttaagctatg ttttatcaag tattggtaaa cctggtagaa ttgatattag
    19561 tgaatatggt tttgattggt acgtatataa tcaatatgga aaagaatttg caatggttgg
    19621 acttgaaaat gatgaagtag tagcacttta tagtaattct attaattcat gtgaaaatca
    19681 agatataaaa ctaaatcaag atagacaaac agtgagaact aagattacac cattaaaata
    19741 taagagaaaa ggaaatacca gatatataat aaactcagaa aatcaatatg atataataag
    19801 taaggaaggt aagtatatta caattttcta cgatattcat gaagaaaata gagtctgttc
    19861 atatttaata attgataaaa gcactgaaga tgagtttaaa aatttatatc cagatgatag
    19921 cgaagaactt aaaaaatgtt ttgaacttga agttatagac cttgtaaata gtgttagaaa
    19981 tcaaagaggg ttaaactcat tgagatacag tgaacaggca actttgagtt caataaaaca
    20041 tagtgaagat atgagagata ataatttttt tgaccatgta aataagaaga atgaaactcc
    20101 atttgacaga atgaaaagag aaggtatagt atatacttca gctggagaaa acatagcagc
    20161 aggacagatt aatgcaatat atgcgcatga agcttggatg aactctgaag gacatagaaa
    20221 aaatattttg gggaactata ataatattgg agttggagta atatttggag gaagctataa
    20281 aacatattac actcaaaatt tttataaata actaaaatat ttgaaaggaa gttaaaaaat
    20341 gacatcatgg aaaaaaaaga cagtatataa gtgcttaata gcagtagtct tgttttgtgg
    20401 aatagtgtta atatctaatt ttagtaaagt ttctgctttg atgatggata ctaatggaaa
    20461 tgttttgatt aaacatggtt caagggaaaa aaagctaata gcaattacct ttgatgatgg
    20521 tcctcatcct aaagaaacta gtcaagtttt agatgtatta aagaaataca atgtaaaagc
    20581 tacttttttt atagctggaa aacatgcaaa atggtataaa gaaccattag taagagcaag
    20641 taaagaaggt cacgagatag gaaatcatac tttcaatcat cctgatataa gtaatttaag
    20701 ttctagtcag atagaagagg agattgttaa gtgtgaagac attttgaaag aagtaactgg
    20761 aaaaaaaccg acattattta gaccaccatt tggaagttat agagaaaaag aattaataga
    20821 aattgcaaaa aagcatgact ataaagttgt tttatggaca ggtgtagatg tgaaagattg
    20881 gaaaaatcca ggagctaata gcattgcaga taaaataatt aataaagtac aaaatggaga
    20941 tataatactt ttgcatgatt atgcaactaa tgatacagtt gaagctttag atatgtttat
    21001 tccaaaaatg attgaaaaag gttttaaatt tgtaacagtt tctgagttga taaaataatt
    21061 tttagggggg agatttacag tatgattctt atgatagaca attatgattc ttttgtatac
    21121 aacctagtcc aatatataga agaattaggg gaaacagttg tagtaaaaag aaataatgaa
    21181 atcaaaataa gtgacattga agaactaaat ccagaggtaa ttgtgttatc tccaggaccg
    21241 tgttctccaa aagaagctgg tatttgcata gatattgttg agcattttaa ggggaaaaaa
    21301 cctatactag gaatttgttt aggtcaccaa actattggac atgtttttgg aggagatatt
    21361 ataaaagcac aacagcctgt gcatggaaaa gtatatagta tcaaccatac taataaagga
    21421 gtttttaggg gacttaaaaa tcctttaaat gtcactaggt atcattcact aatagttgac
    21481 tccaatacag ttccaaaaga attagaaatt acagcaatta ctgataaagg ggaaataatg
    21541 gggattaggc ataagaaata tttaatagaa ggagtacagt ttcatccaga agctattttg
    21601 tcagagtatg gtcatgaaat gcttaaaaat tttataacag aagctagaga aagggtacat
    21661 atgtaatatg ataagagaga taaatactaa attaaattct tttgagatat ttactatttt
    21721 tagaaatgaa catgacagtt ttatattaga cagtgctatg gataaagaaa aattgggaag
    21781 atattctttt ataagtagcc aaccatttaa ggtattaaag tataaagata ctgatgaaaa
    21841 tcctttagaa gttttaaaag aagaactaca taagtatagg gttgtaaatg atactaatct
    21901 tccatttgta ggaggtgctg tgggatattt atcttatgat ttaggtaatt acatagaaaa
    21961 tttacctaga actgctgtag atgatattga gatgcctgat atgtattttg gattttataa
    22021 tcatgtaata gttatagacc atcttgtgca aaaaacttat atagctactc caaacataga
    22081 tatcgaatta gaagaaaaga taatagatga tatagaacaa agaatattaa aagaagagaa
    22141 aaaaggtata gatagcatat gttatgaaga aaaagaagta acatctataa gacttaaatc
    22201 taattttaca aaggaagaat ttaaaaatgc agttcaaagt gttagagagt acataagaca
    22261 aggagatata tatcaagcta atttaacaca aaggttcagt ggagaaacag aacttacaag
    22321 ctttgaattg tatagggact taagaagatt tagcccagca ccatttgggg cattcttaaa
    22381 ctttgaagat gcacatatat tatcaaattc tccagaaaga tttataagat gtgttaataa
    22441 gagaatagaa actagaccaa taaaaggtac tcgtccaaga gggaaagata aagaagaaga
    22501 tttaagactt caacaagaac ttagaaatag tgaaaaagat agagctgaac tacttatgat
    22561 agtagattta gaaagaaatg atattgggag aatatctaaa actggaagtg ttaaagttcc
    22621 agagctattt gtaattgaac catatgcaaa tgtaaatcac ttagtttcca cagttgtagg
    22681 tgagttaaaa gatgataaag atgctactga tgtaataaaa gcaacttttc caggggggtc
    22741 tataacagga gcacctaaga ttagagcaat ggaaattata gacgaattag agccaacgca
    22801 aagaaatgta tatactggtt caataggtta tataggtttt aatggtgata tggattttaa
    22861 tatagcaatt agaacaataa tcaaaaatga caaaaaagtc tactttcaag ttggaggagg
    22921 aatgacttgg gattctgacc cagatgaaga atatcaagaa acacttgata aagctaaatc
    22981 cataatgaag gctttgaggg gatattatga agaataatgt aggttttgat agtagtttaa
    23041 gtaaatttgg aattggtttg tttgaaacta ttagagttaa aaatggaata gcagtagatt
    23101 tagatatcca tatagaaaga atgctaagct caataaattg tttggattta gatataaact
    23161 atacaaaaga tttcttgata aatgaaattg ttacatatat aaaaaaagaa aatgtcatta
    23221 ataaagcact tagaattact gtttttgatg aaggttataa tatatctatt agggatgttc
    23281 catataatca agagacgtat gagaaggggt ttaaattagc tatatctcca atagttaggg
    23341 ggaatagttt aattcataga cataagacta ctaattattt tgaaaacata tataccaaaa
    23401 atatcgcaaa taagactggt tataatgatg gtatattttt aaattcagaa ggtgtaatat
    23461 tagaatgttc tatgagcaat attttcttta taaaaggaga aagagtgtac actcctagtg
    23521 atagattacc gatattaaat ggaataataa aaaaaaggat tatagagatt tgtgacgaat
    23581 tacatataga gttgatagaa aatgaaatta atatatcaga aattagtagt tttgattttg
    23641 tttttgtcac aaatagtctt atgggcgcta taaaggttac agaaattgac aaaataaaat
    23701 ttaataaaga aaatgttgtt tttgataaaa tagtgaagtt attagaataa attaaaatat
    23761 taacaatttg ttggagacaa aagatgatag gttgtattta tgaagtatac aatgaagaga
    23821 taactaaaat aatagaaaac caaaacactt tggctgtata tttagtgggt tctagtaaag
    23881 acgtagattt tactctttat gatgtagaga taaacgatat tgatgtgttc gtttttgtaa
    23941 aagaagggga aaaacaagag agaatactgt ttaaaaaaaa gggaatagaa tttgatgtaa
    24001 attacttttc aaaagatggt gtaaaaaaat tattatcaaa tagggaatat ttctttgtga
    24061 aagaaatgaa agatgctaaa gtactgtttg atagggagaa tatatcacat attataaaag
    24121 atataagtag aaatatttac ttagaaggac ccagtaaaat gtcattagag gaaaagtctt
    24181 ttataaagca agatattgga gcaaaaatat cgaatttaaa aaataaagaa aaatttgatg
    24241 tttttgaata tcattttttg acgaatttat atttaaaaga tataataatt gggtacttta
    24301 atataaatga taaatgggta ccaaaagaca aaaaattgct aaaagtttta aaaaaagaaa
    24361 ataatgagct ttttgaacta gtgtcaaaag taagtgaaaa ttatgactat caaagacttt
    24421 tagatgttta taattatatt tttaaagaaa ttgagacaaa agaggttata aaactaatct
    24481 tctagtcaca aatcaatggg gggagtttaa atgaataaag ttgataaaga aaagatacaa
    24541 catgcagtaa gagaaatact agaggcaatt ggggaagacc cagatagaga aggtctaatc
    24601 gaaactccaa atagagttgc taggatgtat gaagagatat tctctggtct tagtgaagaa
    24661 ccaagagacc atttaaaagt tctttttgca gatgaaaaac atgaagagtt agtattggta
    24721 aaagatattc cattttattc atgttgtgag catcatctag ttcctttctt tggaaaagct
    24781 catattgctt atttaccaaa aggaggaaga cttacagggc tttctaaact tgctagagta
    24841 atagatacat tggcaaaaag accacaatta caagaaagaa tcactaaaaa tgctgcagat
    24901 ataataatgg aagaattaca accttatggt gtcttagtag tagtagaagc tgaacatatg
    24961 tgcatgacaa tgagaggcgt aaaaaaacca ggttcaaaaa ctgttacatc agcagtgaga
    25021 ggaatatttg aaaaggacat tgcgtctaga gccgaggcta tgagtttgat tactatgaag
    25081 tagcaattta ttatagatat aatcatttat tgagataaaa tttaatttaa gggagtgtca
    25141 ttaattgtgt ttgattatgg taaaagaact tatataatgg gaatattaaa tgtaactcca
    25201 gatagttttt ctgatggagg agattttaat aacttggaca tagcaataca acatgcaaaa
    25261 gatatggttg accaaggtgc agatataata gacctaggag gagagtctac acgcccaggt
    25321 catagctatg tagactcaga cgaagagcta agaagagtaa tccctgtaat aaaaaaactt
    25381 aaacaggaat tggatatacc aatatcaata gacacttata aggcagatgt tgcagaagaa
    25441 gcattaaaat taggtgtcac tatggtaaat gatgtttggg gccttagaaa agataaaaat
    25501 atggcttctg taataggaaa atatgatgca gaagtgtgta taatgcataa tcaagatggg
    25561 acaaactatg acaaagatat aatggagtca ataaaagact tttttaaggt aagtatagaa
    25621 atggcaatga gttatggtgt aaaaaaagaa aaaatagtac ttgacccagg tgtaggattt
    25681 ggcaaggatt ttgaacaaaa tattgaagta ttgagaagat taaatgaatt aaaagattta
    25741 gggtatccta ttttgcttgg gacatccaga aaatcagtaa taggaaaagt acttccagtt
    25801 gagcctaaaa aaagattaga aggtacaata gctactactg ttcttggtat aagagatggc
    25861 gtagatatag ttagagtaca tgatgtttat gaaaatttaa tggcagctag aatgactgat
    25921 gcaatatata gaaagtaggt ataatataat ggataaaata ttacttagta atctaggatt
    25981 ctatggatat catggtgttc ttaaagaaga aaattttcta ggtcagaaat tctttgtcga
    26041 catggaactt tatattgatt caagagaagc aggtcttagt gacgatataa ataaatctgt
    26101 aagctatgcc gaggtttaca atgttgttaa agatattact gaaaataaac aatttaatct
    26161 tttggaggct ttagcggaaa atattgctga agaagtcttg aataaattta tacttataaa
    26221 tggagttagg gtcagagtta gaaagcctga ggctcctgta aatggtattt atgactattt
    26281 tggtgttgag ataaggagag caagagatga ataaggctta tttgggaata ggtactaaca
    26341 tgggtgatag atttgataat ttatcaagag catgtgaact tttgaaaaat agtgattcta
    26401 tatataaagt aaaagaatca agcttatacg aaacaaaacc atggggatat acagaacaag
    26461 cagattttct aaatatgtgt gtagaaatag aaacagagtt tgaaccttat gagttgttag
    26521 aatattgtca agaaatagag agagaactac acagagagag aatagttcat tggggaccaa
    26581 gaactataga tgtagacgta ctatttttta atgatgtagt ttccactgat gaaagactaa
    26641 caatacctca tccaaggatt caagatagag ctttcgtttt gataccactt atggatttaa
    26701 atgaaaaact tataataaat gaaaaaacaa taaaagaaca tttaaacctc ctatctgctg
    26761 aagaaagaga agaagtaaag gagcttgtag gttatgaaag aaaacctatt taataataat
    26821 tcaaagatag agataaatac aaataacaaa aagataactg taaaaaaaga taaacttata
    26881 atagctggac catgtgcaat agagagttat gagcaacttt tggagactgc taaatttgta
    26941 aaaagtcagg gagcaaatat tttaagaggt ggagcataca agccaagaac atctccaaat
    27001 tcatttcaag gattaaaaaa agaaggtctt gaaatattaa aagcggttaa agacgaagtt
    27061 ggtatggctg ttattacaga acttatggat gtaagagata tggacgaact gtatagtata
    27121 agtgatataa ttcaaattgg gtcaagaaat atgcaaaact ttactctttt aagtgaagtg
    27181 ggaaaacaaa ataaacctgt tatgttaaaa agaggaatag catccacaat tactgaatgg
    27241 ataggtgctt ctgaatatat agcaatagaa ggaaatagta atattataat gtgtgagaga
    27301 ggtataagaa cgtataatga ttatactaga aatacattag atttagctgc ggtacccatt
    27361 attcaaaagg aaacaggtct tcctgttgtg gtagacccaa gccatgccac tggagttaga
    27421 tatttggtaa aacctatgtc actggcatct tttgcatgtg gagcagatgg aataatggta
    27481 gaagtacatc ctgacccaga aaatgctttg tcagatggtg ctcaatctct ttgctttaat
    27541 gaatttgaag atttaatgaa atcaattaat aattattaaa agaggcaaga gcctcttttt
    27601 tattcgtaat cgttgctgag gtaaatttat aagttataat ttgatagtta gattatttaa
    27661 tataaataaa gatttaatat tgatagaatt aatataaaaa ataaaaattt tttgattaat
    27721 aagaaggata tcaataaaaa aagtcgaatt gtgtatattg agtgattgac aaatttagaa
    27781 tatagtgtag aatagaaatg ttaacacaaa aaattacacc agaaggtgat aatatgaatg
    27841 atataaaaga cattattgaa gaaataaaag ctagatgcga tatagcaagt ataatatctg
    27901 aatatatgaa tataaaacaa tctggagcta attacaaagg attatgtcca tttcatggcg
    27961 aaaagacacc ttctttttat ataaatacat caaaacagat ttataagtgt tttggttgtg
    28021 gtgaaggtgg agacgtaata aattttgtca tgaaaatgga aaatttggat tttatggatg
    28081 ctgtaaaaat tttagcaaat aaatgcggta tagaaattaa tactaacatg aatgaagaaa
    28141 ctaggattaa aatagaaaaa tctaagaaat ttcaagatat tcatacggaa gcagctagat
    28201 tttatctttc aaacttattg ggaagcaaaa atcttgggta tgaatattta agaataagag
    28261 gactagatga taaaattatt aagaaatttg gtttaggatt ttctttggat tcatggaatt
    28321 cacttatgaa tactctaatc agtaaaggat ataaaaaaca agacttattg gagtgcggtc
    28381 taattgcaaa aaatagagat ggaactaatt gctatgataa atttagaaat agagttatgt
    28441 ttcctatatt cgattataga ggaaatataa taggatttgg gggaagagta ttagacgatt
    28501 ctttgccaaa atacttaaat tctccagaca cattaatttt taataaaaaa cagaacttat
    28561 acggattaaa ttttgctaga aaaaatttag aaagtaaaac tatagtctta gttgaaggat
    28621 atatggattt aatttcactt tatcagtatg gtataaaaaa tgtagtagca acccttggaa
    28681 cagccttgac tgaacaacaa ggcattttaa taaagagata tgcggacact gctattatat
    28741 cttatgattc tgatgaagca ggaataaaag cgactttaag agctatagat atacttacta
    28801 aattaggtat caatgtaaaa gtgttagact taaaagatgc taaagaccca gatgaatttg
    28861 taagaaaata cggacttagt gattataaaa aagctatgga tgtttctact cactacataa
    28921 aatataaaat agatcatctt aaaaaagaat ttaacattca aaaagatgaa gaacgagtga
    28981 agttcgcaaa agaagcttct aaaattataa aacaattgac aagtcctgtt gaaattgatt
    29041 tttatacaaa gtatttaagc aatcaaatag atattaatgt cgagtctata aagagagaag
    29101 tttatggtaa aaattacaat aagccatata ataacaaaaa tcaaaagaaa atagaagaaa
    29161 aagtaattga aaaagtcgaa gtcagacaag acggaaaaca attagttgaa gaaactctaa
    29221 taaaaataat gttagaagac aaaaaaatta gagaaatagc gttacttaaa gtagaagaaa
    29281 gtgatttttt attgaaagaa agtaaagaaa ttttaaatta catgattaaa aatcaagaat
    29341 tggacaaaat aactattgac aaattgaaaa gtttaaacat atccgaagaa tacttaaaag
    29401 aattaaattc aatttctttg aatagtataa acttagagaa cacaaaagaa atagaaggaa
    29461 taataacaaa tatcagaaaa aactcattag aagagcaaat taatagcttg ttgagagaac
    29521 aacaagaatt agaaaataat aatgatatga aagaggtaga tggtagagtt atggaaattg
    29581 ctttaaaaat agttgagata aataaaattt taaagagctt gtagagaaag gaggtttgag
    29641 tgtggaaaat aaatcgaata aaaaagagtt aaaaaaggtt actgctaaga cattaataga
    29701 aaaaggaaaa aagcaaggtt ctctgacact tgcagagata atggaagctt tttcggagac
    29761 tgaacttgat aaggatcaag tagaaaatct ttatgagact ttaggaaatt tgggaataga
    29821 aataacagaa acaaaaaact ataaagctga tatagatttt tcggttgctg atgacgattt
    29881 aagtataggc cacttagatg aagatgcaga ggcaatttca catgatgact cttctgcaat
    29941 agaaatagaa actgtggatt tatctttacc aaaagggata agtatagatg accctgtaag
    30001 aatgtactta aaagaaatag gaaaaattcc tctacttaaa ccacatgaag aagtagaatt
    30061 tgctagaagg atgcacgaag gtgatgagat agcaaaacaa aggttagttg aagcaaactt
    30121 aagactagtt gtaagtatag caaaaagata tgtaggaaga ggtatgcttt tcttggattt
    30181 aatacaagag ggaaatttag gtcttataaa agcagtcgaa aaatttgact atacaaaagg
    30241 atataagttt agtacttatg caacatggtg gataagacaa gctataacac gtgctattgc
    30301 agaccaagct agaactataa gaataccagt tcatatggta gagactataa ataagctaat
    30361 aagagtatca agacaactac ttcaagaact tggaagagat ccaaaaccag aagaaattgc
    30421 aaaagaaatg gaaatgacag aagataaagt aagagaaata atgaaaatag ctcaagaccc
    30481 tgtgtcttta gaaacaccaa taggagaaga agaagatagc catttaggtg actttattcc
    30541 agatgatgat gctccagccc cagcagaggc agcagcatat tctttattaa aagaacaaat
    30601 agaagatgta cttggttcat taaatgatag agaacaaaaa gtattaaagc ttagatttgg
    30661 tcttgaggat ggtagagcca gaactcttga ggaagttgga aaagagtttg atgtaactag
    30721 agagagaata agacagatag aagcaaaagc actaagaaaa ctaagacatc caagtagatc
    30781 aaaaaaactt agagattatt tagactaaaa agatacatca ttgatgtttt tgtgataaat
    30841 gccatgagat aagtgatatc tcatggctat tttgtagtaa agatttaatt ttatttttgt
    30901 agttatttca gtctaatatc tataacttgg attaaatcct ataaataaag ggaattgaaa
    30961 ggagaattat atttgaaatt aacagataga ttattgaaaa tagcatcatt agcgtcagat
    31021 ggtaaaaaaa tagctgatat agggacggac cacggataca taccagtata tttgttaaaa
    31081 gagggaaaag taccatttgc agtgctagcg gatgttaata aaggacctct tgacaatgca
    31141 cataaagaag tcatacagaa caatctttta gataaagttg atttacgatt gggttctggt
    31201 atagagatac ttgaaatagg ggaagtagaa gaagtgataa ttgctggtat gggtggaatc
    31261 ttgataagtg agcttctcga agctaaaaaa gaagtagcac ataatgtaga aaaattaata
    31321 ttacagccaa tgcaagcaca agaagaactt agatattacc ttttaaacaa cggatatgaa
    31381 attttagaag aagttttagt gagagaagat tttagaatat atgagataat tgttgccaaa
    31441 tatacaggta aaaatactat aatagaagat gagatatatt atgaggttgg aataaagctt
    31501 ttagaaaaca aagattctct ttttaatgat tttatagaaa aaaagataaa gacatatagt
    31561 tctatagtta ataaattaga agggaaaaat ggggaagcta tagataagaa gagaaaggaa
    31621 agcgaagttg caataaaaaa acttgaaaat ttaataaaat aaataggagg tatatatgct
    31681 attaaagagt ctaactagaa aaattgaaaa aaagtatcct ctaaacttag cagaggattg
    31741 ggataatgta ggtcttatag ttggagattt tgatatggat gtaaaaaagg tattggtatc
    31801 tttagaagct aatgaagatg ttattgatga agctatatcc aaaaatatag atttaattgt
    31861 gacacatcat ccttttatat ttgggaaaat taataaaatt aatagtggag atttaaaagg
    31921 cagacttatc caaaaactta ttaaaaatga tatatcactt tattctatgc atacgaattt
    31981 tgatatagca tttgatggtc taaatgacta ttttatggaa attatggaat ttggtaattc
    32041 aaaggtttta gatataacta aaagcgaaaa tttgtataaa ttagcagtat acgttccaca
    32101 taactattca gatgaactta gaaaggtttt aagtaattca ggagcaggtc atatagggaa
    32161 ttatagtgat tgtacgtttt caacagaagg tgaaggacaa tttaagccat tagaaggctc
    32221 taatccgttt cttggaagtg taaatgatat agaaactgta aatgaagtta aaattgaaac
    32281 tgtagtacca cagaagcttt taggtggggt aataagttct atgttagatg cacatcctta
    32341 tgaagaagtt gcatatgatt tgtacaagtt agaaaataaa ggtgagatat ttggactagg
    32401 tagaataagt aaacttgata aaagtatgac attagaaagt ctttcaagga aaataaaaga
    32461 gaaattaaat atgaaacaca taagagtagt tggaaacctt agtacagaca taacgaaagt
    32521 agccgttgta acaggagctg gttcagaatt tgtaaaaaaa gctaagagac aaggtgcaga
    32581 agtactaatt acgggtgatg taaagtatca tgaggcacaa gatgcacttg atattggaat
    32641 gtgtatagtg gattgtggtc attttgatac agaagatata ttcaaaaatg ttatgaagag
    32701 atttttagat gaatttagtg aaattgaagt aataaagagt aatgtatatt tgaatccatt
    32761 tagtactatt tagcagtgtt tgataataaa aatactatat aaaaaataaa tttgagttta
    32821 attaggggga taatgatgaa agtagcagta atttttcata gtgtttgtgg gagtacatat
    32881 ttattagcta gagaatataa agaagcgctt gaagaaatga atatcgaggt agatatattt
    32941 agagtaagtg acgaagttgc aaaaacactt cctcaatatt atttgataaa ttccaaagaa
    33001 tacaaagacg aatttgaaag tataaatgta attaaatctg gaaaagaaat attagattat
    33061 gatgctatat ttatgggttc accaacatat tatggaaatg tatctggacc aatgaagatg
    33121 tttatggata gtttttctga tatttgggta ggtgctcctt tgagtggtaa aatatttggt
    33181 tgttttgcta cagcaggttc acaacatgga ggaggagaat tagctttgca agctatgaat
    33241 atatttgccc aacatatggg aatgactctt ttgtcagttc catgttcagt aagaggaggt
    33301 tatccagcgt atggaatact tcatatagca ggagataatt ctgatataag accaaatgat
    33361 gatgccaaga tgggtataag agattatcta aaaagactta acatataaga atatcatata
    33421 aaaataccta aaagcgactg tatatacttt taggtatttt tacttaatta taaacgtgaa
    33481 gctttaccag aaatatggtc aatttctatt ttaacaacag ttgttaagtt catatccttc
    33541 tctatgtact tcatgccttc ttcaaagaat cctttagaat atttttttat tatttctaca
    33601 agagcatttt tcttttcttc attttctaca gtgcaagctt ttccaaaaac tatagcacta
    33661 gaataagtag tactaaattt atctggaata acactttcat tagcaactac taaaaaagaa
    33721 actttgttgt ttttcgatat attatctaac ttatgcccat ttcttgcaca atgaaagtaa
    33781 atagaatcat taaagtatac ataatttaca gcaactccat aaggataacc attttcagat
    33841 atagtggaaa aagtaccaaa ttcaccattt tttaatactt caacagtatc ttcttttgtc
    33901 atttctcttt ttttcaatct catttcttta aacataatat catcctttct aattttgatg
    33961 cttaaattaa attttagcac atttataaag attatgctat gttttttaca aattttaaga
    34021 aaaaaatata gtttaagata aaaggtagtg aaagagaagc tatttggagg tttatttatg
    34081 atattattgt tagagtagta taaacaatac taataaattt aatagctggt ttcttgttat
    34141 ttaaggtagt aagcatgtct ataagaaggg gaagttatcg agggtagaag ttgttattta
    34201 taacgatatt ataaagaaaa tattattata taaaataata aatagtaaag aggtatcaag
    34261 ttgaatataa tacttaatac ctcttcaaaa ttaagttata aaagtgttgt gggaattgtc
    34321 ctatacatgg taaggagata ataaagaatg tatctttaaa gtctcaacta aacagcaaaa
    34381 tttatccaac aaacatacaa taaatgcttc tttgtatcta ggtggcttaa cagtaagtgg
    34441 ccacatgtgt tttaaaatta tgtccgtttc tttttcattt atttgaaaaa ataaagttgc
    34501 attttttaat gcttctctag ggtgagtaaa tccatgtaat ccttttctat cacctttgta
    34561 atgccaatca taaagaaaaa agtcgtgtag taaagctcct ctaataacac tttgaatatc
    34621 aacacttaag tgcaattttt tacataatag ataactataa tatgctactg acaaactatg
    34681 ttcaagacat gaaacattgc catgatgagg aaatgttctc ataagttgaa ctttttcaga
    34741 agaaagtatc tcagaagcac attcattaaa atatttgatt tcagcttttg acaacattat
    34801 gaaaatctcc attcttagta atatataaat actatacttc aacgataata tgaaatattt
    34861 cttatcctac aaaatataat acccaataat acaagtaaga taccttttat ggtattttaa
    34921 agtaagagag attttattag gctaaacaat gaaaaaatta aaagtaaagc tatagcaata
    34981 tatagtattt ttccaagagg attgccaaaa tttatagtcc atccaatacc aaacctttta
    35041 ttaaccatta atgatgggtc atttggatta ttatacatga tgcctgctat ccaatacttc
    35101 tcatcttctt ctggagaata agatgagttg aattttaaat ttggggaatt aatatatgtt
    35161 gctattaaat aaattataac taaaagcgtt gtaatattca tagcagttga tagttttgtt
    35221 tttattgata aaagagtagt aaaaaactgt gttgtcatta taatcatcaa tattaaaaac
    35281 aaataaccaa ttttatttaa atatttaatg ttttcaactc tactttcttc aattctattt
    35341 gtatctatct taatccttga ctttatggat ccaatggaag ttatatataa taaaaccatt
    35401 aaacaaaagt cagtaccgat taatttaaat acattaagaa atgatttatc tataaaaaca
    35461 tcaggagacc catttatgtt ccaatgagtg gcaattgagt ctggcaattg attataatta
    35521 aatgctataa aaatactcat tcccaaagta aatagtatcg gtattaaata gattatttta
    35581 aattttttta ttattttatt tttcttattt ataaaatcca tatctactat taattttgaa
    35641 tctatatcta tatgacctaa tttagcataa atttctaatt taagcttctt agctttttta
    35701 tgtgtatgaa tatatagtat actttcatat aataaaaaac ctataatacc aataaaatag
    35761 gaaagttcca atttattaaa tgtaaaaacc agaataaaaa ctaatataaa gtctataata
    35821 aaacctaaag ataatagttt tttaaatttt ttatctaact ttataaagta attataattt
    35881 ttatgctctt ggtctataga aactccatag atgtgttttt tacctacaaa atagggcata
    35941 aaataaaatg tagcatacat caaaattaga actggaaata ttgtaaatag acattcaatc
    36001 ttactcataa tatttcacct catacttgtc aaataaactt gaacttagtg acaaaaattc
    36061 ttctttacta atacctgata aataagattt tgccaccaat aattccaagt caagtttatt
    36121 tttttgacga gttgtattat ctattttcat tggtagttga gctacaatag caccttttct
    36181 tctatcaata tttaagaatc cctcttcttt taattcattg taggatttgt tcactgtatg
    36241 taaatttaca ccaatatttt cagccatact tcttacagat ggaagtgatt catttatttt
    36301 caattctcct gaagctatag ccttagttat ttcttcttta atttgtacat aaatagattt
    36361 atcactatta aaatctaaat ttaaaaccat aaaaaacctc cattatgctt tataaatgaa
    36421 aacgattgtt atatacatag tataacataa aaaataataa caaacacaaa atataagttt
    36481 atattttgta aaaattgtaa tttaaaaaat aacataattt ttattaaaat aatacagata
    36541 ctatttatag atagaaataa aaattagaaa ggagaaaaaa tatgttaaca tatttaatga
    36601 aaagacaaat gcataaaaaa gatgatggaa tgagtaaacc aatgatgatt acaggagctg
    36661 taattacagg tgtaggagca tatgctgtat ataaaatggt aaaaagccat aaatctaatt
    36721 ctcaaatggt caatacaaat tattatggaa atagcgatta tgatgattat aaatatgacg
    36781 aatttgatta tgactgtgat tgtagagaca caaaacaaga ttatgaatat gaagaattaa
    36841 aaaaaaaagt aaaagaattt aattcaagaa gaataaattg tgaaaattgt ccacatgttt
    36901 ctcaagaaga aatgatgcaa tatgtaaata gtgttaaaga tgtaaaggaa gagattgatt
    36961 tacatgaaga tgctaaaaaa gataatattt acaaagaaga tgaatacaaa aaatatgaag
    37021 aagaataagt aatattttgt ataactgaac aaatagtaat gtcaaattat ttaatatcta
    37081 gttttaatat gtaagttgta acttaacttg aaagaatcag tatcattctt tagatagtta
    37141 agtaacaact tacattttat ttttataaat tttaatagtt ctttgtgact attttgtaat
    37201 tttagaaata ttttagtatc catggtaatt gtgtataata aaacttgtat agatagatag
    37261 tatgaaatta agcagatagt ttaaaattaa aatgaaaaga aaggaaatag ttatgttaaa
    37321 gttgttgata gttgaagatg acagtactat atcttttgga ataaagtatg ctttagaaca
    37381 agaaggattt agtatagata tatcaaagga tttatctagt ggaaaagaaa tgatttcttc
    37441 taatgaatat agcatgatac tgttggatgt tacattgcca gatggaactg gttatgagct
    37501 ttgtcaatat ataagaggat tctctcaggt tcctataata ttcttgactg cttgtgatga
    37561 agaagtaaat attgtaatgg gtctcgatat aggtggagat gattatataa caaaaccttt
    37621 tagaattaga gaattaatat ccagaataaa agctgtttta agaagaaaag gtaatacttc
    37681 agaagaaaat aagaaaatac ttaaatttgg agatttaagt atatatacat tagaagctag
    37741 agtatataaa aatgataaag aaatatttct gacatcagtt gaatataaac ttcttttaat
    37801 tcttatacag aataaaaata ctgttttaag tagaactcaa atattggaaa aactttggga
    37861 tgtaacttat gattttgtaa acgacaatac tctaactgtt tatataaaaa ggcttagaga
    37921 aaaaataggt gatgatttat gtgaaccaat ctacatagaa actgtaaggg ggattggtta
    37981 taaatggata ggaagtgaaa atagtgtttc tatataatcc agaagtgaag aagtttttaa
    38041 gtaagtatgt tactttgatg tttattgtta tcataatatc tattggattt agcataatca
    38101 acgtgtcctt aactaaagag atggttgtaa gaaataatca ggctataata ggaactttat
    38161 caagcaagta tccaaacttg gaaagtcaga tagtagacat aataactcaa gggaagtcta
    38221 tggagaacat agattatggt cagaaaatat taattaagta taattatgat aaatctatta
    38281 ggataaactc tgagcctatt atttctaaat tagttttaga gactatcaaa ataaatataa
    38341 tattagtatg tataatattt attttgatat ttgtgttggt tgttagatat tttacgtcaa
    38401 tttacaatga tttatcagat atgactaagt atgtttacta tagttcagag ggtaagccat
    38461 ttgatatgaa aaacaagaat caagaagggc agataggtct tctaaaaact gaactcttaa
    38521 agatgacaac aattcttaat gaaaaggttg aacttttaaa aagagaaaag atatttttaa
    38581 acaatacaat ttccgatata tcacatcaat taaaaactcc tatgacttct ttaataatgt
    38641 tgaatgattt attatataat gatgtaccat acgaagtaaa aatcgatttt ttaaataaga
    38701 taaaaaatca attaaataga atggattggt tgattaaaag catgcttaaa ttatcaaagg
    38761 tagaagcaaa agtgataaat tttaaaaaag ataaggtaaa atttagtgaa ctcatacata
    38821 gagcaatgct atctatgaaa atacctatgg agattaaaaa tcaaaaactg actatagagg
    38881 gaaatgataa tgtatcttat attggtgata ttgattggtc cgtggaagca ttagtgaata
    38941 taatcaaaaa ttgtgtagaa catactcctg aatttggaaa tataattata acctataaag
    39001 aaaatccatt attttctgaa ctaataataa aagatgaggg tgaaggtata gataaaaaag
    39061 atataccaca tgtatttaaa cgattctata gaggaagaag tagttcaaaa gaagatagtg
    39121 taggaatagg tcttgcaatg tcaaaatcta taatagaaag ccaaaatggc gatatatatg
    39181 taaacagcga aaaaggtaag ggtacagaat ttcatataat atttcataaa atgtatgata
    39241 gtgactaatc tgtaatattt aattcacttt atagtaatag ccaaaaccta atatatgaag
    39301 tatagaatat aaatattgga ggtagttatg gaaattttaa gagtagaaaa tctaactaaa
    39361 agttatggta aaaatgaaac aaaagttgat gctataaaaa atgtaagttt atcagttgaa
    39421 aaaggaacgt ttatagcaat aacaggtcca agtggaagtg gtaaaagtac cttattacat
    39481 ttattaggag gagttgacag accaactagt ggtaaggttt atataaatga tgttgatata
    39541 tataatttaa aaactaagga tttggcgata tttagaagaa gaaatatagg tcttatatat
    39601 caattttaca acttaattcc agtcttaaat gtgaaggaaa atatattgct tccagcagag
    39661 cttgacaata gagatattga tagagaatat tttgatgatt taatgaaaac attaggtcta
    39721 attgatagag aaaagcacct tccaaaccaa ttatcaggag gtcaacaaca gagaacctct
    39781 ataggacgtg cattaattaa tagaccttct ataatattag cagatgagcc aacaggtaat
    39841 ttagatagta aaaattcaaa ggaaatatta gaattgttaa aattgtcagt aaaaaaatac
    39901 aaccagacac taataatgat aactcatgac aaaaatatgg cacttcaagc tgatagaatt
    39961 ataagcatag aagatggaat cataaaaagt gatgaggtga tgtagatgaa tttatataca
    40021 tcactaacta ttagatactt aaaacaaaat aaaagaagaa ccattgtaac cataattggt
    40081 ataatacttt caacagcttt aatttgtggt ataggaaata tatttgaaag ttttatggat
    40141 tatcaaatga gagaaactat aaagaatgat ggaagtttcc atgttacatt ttatgatgta
    40201 aatagaaaaa atgtggaata tgtaacgaaa tcagctgaaa tagaaaaaca tgcatttacc
    40261 aaacaacttg gatattctaa attagaaaat agtgaaaatg ctatattaag tataaagcaa
    40321 tatgataaaa atgcaataaa cggatataaa gtttctataa aagaaggtag atttccagct
    40381 aaggaaggag aaattgtttt aagtgaaagt attattaatc ttatagatga taagttaaaa
    40441 ataggagata agataacttt aaaagtaggg gatatgtttg actcaagcaa gaaaaagatt
    40501 gatagtatga catttcatga aggtgattat atagcaaatg aaaaagatag aacttttaaa
    40561 gttgttggta taattgaaaa acctgggttt gaacgttata atggaattgc aacagctata
    40621 gcctacttta atcctatgaa taattacaat gatacaatta atgtatcagt agcagtaaga
    40681 aatcctaaag atgtgtataa gataagtaat aaaataagta aaaatatata ttcaaataaa
    40741 gataaagaag cagtttccga tatgataaaa tacaatgaac atttattgag gttacaaggt
    40801 gctagtaagt attcaaatat aaatagctct ataaagtcta taattatagc agtaactatt
    40861 ttagtaatca tatgtacaat tgctacagtg tacaattcct ttagtatttc tataaatgaa
    40921 cgaaaaaaac agtttgggat attaaattct ataggtgcta catctagtca aattaaaaga
    40981 ttagttttta tagaaggtat tataattagt ttaattggta taccaatagg actaatatct
    41041 ggaacagtag ctatagacct attgtttaaa attataaata agtattttac tgaatcggtt
    41101 gttactaaga tgagtttgca gatagtgtat aatccaataa taattattgt tagtataata
    41161 atagttttgc ttacaatctt tatatcaatt ctactacctg caatatcggc ttctaatatt
    41221 tctcccctta atgtgattaa aaatactgga gagcataaag tgggcaaaat caaaagctca
    41281 agacttatta aaatgatttt taaaactgaa ggagtacttg catataaaaa tattaggaga
    41341 aatagaaaaa aatttataat aactcttttt tctttaatgg taagtataat aatatttata
    41401 tcttttagtg gctttacatt acttttacta agaggtgaag aaattagaaa ttctcaaaga
    41461 aaatatgatt tgtatttaac tgcaaagaac acagctaggt ctgtagagga tactataagt
    41521 gaactagaag atattgatgg gataaaaaac tttgaattgg caacaggaca atatgtgtct
    41581 ataagagtaa gtgaaaataa aataaataaa tccaaggaag atttaatcag gaaatattat
    41641 caaaaatata agattggaga tagttatgaa tatgatttca gtaataatga attaattttt
    41701 ccaggagatt ttgcagtaaa aaacaaaata aataacttag tacagggctc ttttaataaa
    41761 gagagagcaa ttgaagaaaa tggtgttata ttagttagaa aaagtgcatt tgaagaaaat
    41821 gggaagaaag gtgtagtaga acttaccaac tataaagtag gagatacagt aaattgtgaa
    41881 tatcttgatg aaaatgcctt aagtaaaaaa gtaaaggtca agattcttgc tataactgat
    41941 gaagaacgtc taggtcttgg ttatcaaaat atggggttac aatttataac ttatgatgaa
    42001 gttgctaaaa atttaaattt aaaattaagt aaaagcctta tatttattga ttcaggagga
    42061 agcattaaga ctaagaagga aatagaagct ttggcaaata aaaataattt taacttttat
    42121 gatgaaagtg caagcaatga aagtgaaaaa caagatttaa aggttattaa aatatttgta
    42181 tatggtttta tagtagtgat atctttagtg agtgttacaa atattctaaa tacagtaagt
    42241 acaagtataa atctaagaaa aagagaactt gcagtaatac aatctatagg ggttactcct
    42301 aaaggattta ggaaaatgat atatttagaa agttttatat atggtatact gtcattgttg
    42361 tttggaatac caattagtat tggtataatt ctaattatga ataaacttat ttcaggtgta
    42421 attgaatttt ctccgattat accgtggaca gccatagtta tctgtatagt aagtgttttt
    42481 ataataactt ttatagcagg gtatatacca atgagtaaat taaataaaga aaatata
//
LOCUS       DAENCV010000026        41561 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAENCV010000026 DAENCV010000000
VERSION     DAENCV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.026"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            243..1673
                     /locus_tag="KQD82_001293"
     CDS             243..1673
                     /locus_tag="KQD82_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG4379771.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2041..2619
                     /locus_tag="KQD82_001294"
     CDS             2041..2619
                     /locus_tag="KQD82_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG4379772.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2649..2795
                     /locus_tag="KQD82_001295"
     CDS             2649..2795
                     /locus_tag="KQD82_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG4379773.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3100..4479
                     /gene="scfB"
                     /locus_tag="KQD82_001296"
     CDS             3100..4479
                     /gene="scfB"
                     /locus_tag="KQD82_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG4379774.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4508..5005
                     /locus_tag="KQD82_001297"
     CDS             4508..5005
                     /locus_tag="KQD82_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG4379775.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5303..6967
                     /locus_tag="KQD82_001298"
     CDS             5303..6967
                     /locus_tag="KQD82_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG4379776.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6980..9430
                     /gene="recJ"
                     /locus_tag="KQD82_001299"
     CDS             6980..9430
                     /gene="recJ"
                     /locus_tag="KQD82_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG4379777.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9443..9955
                     /locus_tag="KQD82_001300"
     CDS             9443..9955
                     /locus_tag="KQD82_001300"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG4379778.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10029..12236
                     /locus_tag="KQD82_001301"
     CDS             10029..12236
                     /locus_tag="KQD82_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG4379779.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12261..12710
                     /locus_tag="KQD82_001302"
     CDS             12261..12710
                     /locus_tag="KQD82_001302"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG4379780.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12725..13339
                     /locus_tag="KQD82_001303"
     CDS             12725..13339
                     /locus_tag="KQD82_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4379781.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13430..14986
                     /locus_tag="KQD82_001304"
     CDS             13430..14986
                     /locus_tag="KQD82_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG4379782.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIKNMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14976..16238
                     /locus_tag="KQD82_001305"
     CDS             14976..16238
                     /locus_tag="KQD82_001305"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG4379783.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16312..18099
                     /gene="aspS"
                     /locus_tag="KQD82_001306"
     CDS             16312..18099
                     /gene="aspS"
                     /locus_tag="KQD82_001306"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG4379784.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            18558..19790
                     /locus_tag="KQD82_001307"
     CDS             18558..19790
                     /locus_tag="KQD82_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG4379785.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            19804..20610
                     /locus_tag="KQD82_001308"
     CDS             19804..20610
                     /locus_tag="KQD82_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG4379786.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(20722..21591)
                     /locus_tag="KQD82_001309"
     CDS             complement(20722..21591)
                     /locus_tag="KQD82_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4379787.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            21963..23390
                     /locus_tag="KQD82_001310"
     CDS             21963..23390
                     /locus_tag="KQD82_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG4379788.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNDLNSQTEKELK
                     RLGVKKVYIIGLSSSISNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(23466..23735)
                     /locus_tag="KQD82_001311"
     CDS             complement(23466..23735)
                     /locus_tag="KQD82_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379789.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(23738..25180)
                     /gene="nhaC"
                     /locus_tag="KQD82_001312"
     CDS             complement(23738..25180)
                     /gene="nhaC"
                     /locus_tag="KQD82_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG4379790.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFVVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(25205..26371)
                     /locus_tag="KQD82_001313"
     CDS             complement(25205..26371)
                     /locus_tag="KQD82_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4379791.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(26596..28716)
                     /locus_tag="KQD82_001314"
     CDS             complement(26596..28716)
                     /locus_tag="KQD82_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4379792.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMEYDYNFKKSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILIGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            28944..29345
                     /locus_tag="KQD82_001315"
     CDS             28944..29345
                     /locus_tag="KQD82_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379793.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            29388..29681
                     /locus_tag="KQD82_001316"
     CDS             29388..29681
                     /locus_tag="KQD82_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG4379794.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            29696..30136
                     /locus_tag="KQD82_001317"
     CDS             29696..30136
                     /locus_tag="KQD82_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG4379795.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(30228..30701)
                     /locus_tag="KQD82_001318"
     CDS             complement(30228..30701)
                     /locus_tag="KQD82_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG4379796.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(30701..31489)
                     /locus_tag="KQD82_001319"
     CDS             complement(30701..31489)
                     /locus_tag="KQD82_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG4379797.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            31817..33061
                     /locus_tag="KQD82_001320"
     CDS             31817..33061
                     /locus_tag="KQD82_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG4379798.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            33384..34481
                     /locus_tag="KQD82_001321"
     CDS             33384..34481
                     /locus_tag="KQD82_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG4379799.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            34472..35170
                     /locus_tag="KQD82_001322"
     CDS             34472..35170
                     /locus_tag="KQD82_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4379800.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            35173..36237
                     /locus_tag="KQD82_001323"
     CDS             35173..36237
                     /locus_tag="KQD82_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG4379801.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            36420..37124
                     /locus_tag="KQD82_001324"
     CDS             36420..37124
                     /locus_tag="KQD82_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4379802.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            37117..38439
                     /locus_tag="KQD82_001325"
     CDS             37117..38439
                     /locus_tag="KQD82_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4379803.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            38605..39978
                     /locus_tag="KQD82_001326"
     CDS             38605..39978
                     /locus_tag="KQD82_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379804.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISASAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(40350..41288)
                     /gene="pdaA"
                     /locus_tag="KQD82_001327"
     CDS             complement(40350..41288)
                     /gene="pdaA"
                     /locus_tag="KQD82_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG4379805.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        16023 a         4716 c         7073 g        13749 t
ORIGIN      
        1 agaaaaaatt tgaaaaaaat cactataaat gagtaataaa tataaatagt gtatactaat
       61 gggatggaaa atgtccacta ttaactttat taccccacat atataagata aaattattaa
      121 tataaaatat aatctatcga caatgaatag acttttaaat aaacttaata taaaattaat
      181 taaataagtg cttaaaaacg atttacttta atgatagaat catattaaag gaggataatt
      241 ttatggatat aaagaataaa attaaatcta tatttttacc agaaaaaaat gactatttag
      301 atgatgaaga atttgagtat gatatagaaa ataatgaaga agatttaaag gaagataaca
      361 aagtacattt aaatgattta agcagagtaa aatatgatta cagagagtta aaagacatag
      421 aaaaccaaac agaagagatg tgtctgctag cagtaaagca ggatggtact ataattgagt
      481 ttgtaaagga caagacttat aaagtatgta tggaagcagt aaaacagaca tataaatctc
      541 ttaagtatat aacaaatcaa aatgaggata tttgtataga agcagtaaaa cagaactaca
      601 gagctttgta ctacataaat aataagacag aaaatgtact aattgaggct ataaaaaatg
      661 catcaactta tgatgtcatg gaagtattta aatttgtgga agagcaaaca gaagatgtat
      721 gtctagcatt tatagaaagg gcatctaaaa atgatgttgc agagatatta aaaggaataa
      781 aagagcaaac tccagccata tgtttagaag ctgtaaaaaa agatggaaaa tctttagctt
      841 atgtaaagga acaaagtaat agtatatgtt tagaagctgt aaaagaaaac tacagtgcac
      901 tatcttgtgt aaaagagcaa actgaagaaa tatgcataga agcagtaaaa cagaatgact
      961 ttgcattgta ttatgtaaat gaacagacag aaaaaatatg tatggaagca gtaaaaagaa
     1021 gttatatggc tctacagtat gtaaataagc aaacagaaga gatttgtttg gaagcagtaa
     1081 gaattgatgg aagagcatta cagtacgtaa aagagcaaac agaagaaatt tgcttggagt
     1141 cagttagaca aaatggaaaa gtactgcaat atgtaaaaaa acagactgaa aatatatgta
     1201 tagaagcagt aagaggttca tttgaagaac ttgaaataaa agaaatatta agttatgtaa
     1261 agataccaac agaaagaata tttgtagaag cagtaaagca aaatggaaaa attcttaaat
     1321 atgtagaaaa tcagacagaa ttaatatgtt tagaggcagt aagagaaaac tataatgctt
     1381 tagcttatgt aaaggaacaa acagaaaaga tttgtttaga agcagttaat caaagttatg
     1441 aagcgcttaa atatgtgaaa gaacaaacag aagaagtttg tctaaaagca gtaaaacaag
     1501 attatagaat gttgaaatat gtaaataatc aaacagaaaa aatatgttta gaagcagtaa
     1561 agcaaaatta tagagcttta gaatttgttg ataatcaaac agaaaaagtt tgtcttgaag
     1621 cagtaaaaca gaatagaaaa gcactacagt atgtaaaaca aaaacaaagt taaaaggcta
     1681 aaatatatag aaaatatatg caaataaata aaatttgata ttttgtaata aataaaatgg
     1741 gtatctcaaa atgaactttt tagtttgtga gatactcttt ttatatgaaa taaaatatat
     1801 ttttagcctt ttaacaatag ggaaaaggtt tatttctatt ttgacatagg ctttttatgc
     1861 atataaagct tgataaaatt gttttattat aattgttttg ttatatgaat aataagaaga
     1921 aaattatata ggtctttttt tgaccagttt tacattttta acaactagtt ttgtaaagta
     1981 tattgaattt tctcaaaaaa gaatgtactc tatatatgta aaattttttt ggaggtaaaa
     2041 atgtttaaaa gatatgctga aaaaatgaca tctgttttaa tttgtaataa tatgatagat
     2101 aataatgaat ctaaagtcta ttcttatgga tttgaaatat taatagcttt catagttaat
     2161 ataaccacca tgctgtttat aggtttttta tttggtaaat ttacatatgt attatttttt
     2221 ttaatgtgtt attgtccaat aagacaattt tcaggagggt atcatgcaga caattatttt
     2281 agatgcttat taacctttat ttttataatt ttatctacaa tcttaattat agaaaatata
     2341 aatatagact tatttaaaaa tattattatg attattgcaa gtgttagttg ggttggaatt
     2401 tgtgttctgt gcccaataga acataggagt aaccctataa gtgataggga aaagttggtt
     2461 tataagaaaa ctgctatatt tatatctaca gtagtgttat tgataacaat tttaagtctt
     2521 tctttgagta tatttgtaga ttatttcaca tatagcgcat ttgccatgtt ttggattttt
     2581 gtgatgttag tattaggtaa gttaaaagct aaagtataag taaaatatgt atgagggaga
     2641 gttaagttat gaaaaagttt attgtaagat ttatgaagtt tgctagctca ttggcactta
     2701 gcactgctat tttgtcagct aatagtacct gtccttggat tattcatcag cctaaagtac
     2761 ccaaagaaat cagcaatcta aagaagacaa attaaataca agcacttatt tatacaaaat
     2821 tatcaggaaa aaagaaatta ttatatttta ataagattaa aacaatataa aatcagatac
     2881 aaaatttatt ttatttattt tataggagcc taatacctat ttggtaatat attagtgcgc
     2941 caatttaggg ggatatagtg tatttaatga aatgacttac ggagagtaag tcatttttgt
     3001 attgtatgta ttttatgtaa acttatgata taatgaaggg taaagtttaa ttaaaataat
     3061 aaaaaacttt agtatagata gtaatttagg aggtagggta tgagtatgat acataaattt
     3121 tctatgaatg gatataatat cgttttagat gtgaatggtg gagctgttca tgtgttagat
     3181 gatgttgcat acgatttgtt agatttttat aaagaaaaaa gtaaagaaga aatattagag
     3241 atattaaaat caaaatatca agaagaaaaa ataaatgaag catatgaaga aatattaaat
     3301 ttggaaaaag agggtctttt atatacagaa gatacatatc aatatcatcc aagctttgta
     3361 catagagagc ctgtagtaaa agctttatgt ttaaatgtag ctcatgattg taacttaaaa
     3421 tgtaaatact gttttgcagc tcaaggtgat tttggaggag aaaaagaact tatgagtttt
     3481 gaagttggta aggcagcaat tgactatttg attgctaatt caggaagtag aaagaaccta
     3541 gagatagatt tctttggtgg agagcctttg atgaactttg aagttgtaaa acaactggtt
     3601 gactatggaa gaagtgtaga aaaagattat aataaaaata taagatttac aataactaca
     3661 aatggtgtac tgctaaatga tgagattata gattatataa atgaaaatat gcataatgta
     3721 gttttaagtt tagatggaag aaaagaagtt aatgataata tgagaccaac tttaaatgat
     3781 aaaggaagtt atgatattac attaccacga tttaagaaac ttgtggaaaa aagagcaaaa
     3841 gataagtatt attatataag aggtactttt acaagagata atttagattt ttcaaaagat
     3901 gttatgcatt ttgcagattt aggatttaaa ttaacctctg tagagccagt cgttggagat
     3961 gagagtaatc catatgcact tagagaagaa gatttaccta agatatttga agagtacgaa
     4021 aaatttgctg tagagtatgc agatagacaa ttacaaggag atggatttaa atttttccat
     4081 tttatgatag acctaaatca aggtccatgt gtaataaaga gaataactgg atgtggagct
     4141 ggaaatgagt atctttctgt aactccaaat ggagatatct atccatgtca tcaatttgtt
     4201 ggaaatgaag aatttaaaat ggcaaatata tttgatgaag aaatagtact tccagaaaat
     4261 ttaaaaaata tgtttagaga agctcatgta tatactaaag aagagtgtaa acaatgttgg
     4321 aataagtttt actgttcagg tggatgtcat gcaaatgcga taaactttaa taatgacata
     4381 tcaaaaccat atgaattagg atgtgaaatg caaagaaaac gtacagagtg ctctataatg
     4441 atacaagcta agttaatgtt agaaggagca accaactaaa tttatatttt aaaggagttg
     4501 tttatatatg attagagatt atttagaaga taaaccttta attgacgaaa gtgtatttgt
     4561 ggcaaaatca gcagatgtaa ttggaaatgt taaaataggc aaagattcaa gtatatggta
     4621 caatgcagta gttaggggtg atgaaggacc aataactatt ggagaaaaca ctaatataca
     4681 agattgttct atagtgcatg gagatacaga aactataatt ggtaacaatg taacagttgg
     4741 gcataggtct atagttcatg gttgtaaaat tagtgataat gtgttaattg gtatgggttc
     4801 aataatattg gataatgctg aaataggaga atatactctg ataggagcag gtacactaat
     4861 tacatctaat aagaagtttc caccaggtgt tttgataatg ggttctcctg gtaaggttgt
     4921 tagagagctt acagaagaag ataaaaaata tattgatgaa tcttatgaat ggtatttaga
     4981 ggcagcacaa aatcaaaaat attagtttaa agagtggttg tttcaaaaca tatttaaatc
     5041 tgttttgagg caaccactaa tttattttat tagattttgt tgatatttta gttttcaaaa
     5101 agtatttgat taaaccagat ataaaatgtc aaaaataaaa attaactgag ttaaataatt
     5161 ttaataaaat tggtagtata tgtgataaaa ttaaatatta aagaggatat aggatattag
     5221 aatatcaatt ggtgaaataa tgtcatatac gataaattaa ggtgtaagtt tttgggtata
     5281 cctatcttag gagtgaaaag atttgagaat aagttataga aatttaaagc gatatagaga
     5341 aattggatat gttttaataa agtatggttt tagttttatt gtagaaagac taaatataga
     5401 aggaattgcc tataaaatac cactatttga cccaccagaa gaaataaaaa atatgacaac
     5461 tggtgagaga atgaaaagag ttttagaaga actaggacct acatatataa aaataggaca
     5521 gatattgagt acaagaaaag acttgttaga ccaggatata attgatgaaa tatcaaaact
     5581 cagagatgat gtagagaaat ttgattcaaa tatagctatt gatatattta aagaagaagt
     5641 gggtttaagt atagaagaaa tattcttaga atttaaagaa gaaccaatag cagcagcttc
     5701 aattggtcaa gtttatgaag gtgtacttaa aacgggagaa gaagtaatcg taaaaattca
     5761 aagaccaaat atagagaaaa taataaaatc tgatttagag attcttagaa ctattgcaaa
     5821 tactttaaaa gatttaaaaa aagattttaa tttagattta gtacagatga ttgaagaatt
     5881 tcaaactcaa ttaatgaggg aacttgatta tacatttgaa gctataaatg caactaaatt
     5941 ctctaggata tttaaaaata gtgatgaagt atatatacct aaagtatatt cagaatataa
     6001 tactaagaaa attttagtaa tggagaaagt taatggtaca aagttaagtg atgtagaaaa
     6061 aataaggaga ctaggttata acacaaaaac tattgttgaa ataggcgtta ggtcattttt
     6121 tacacaggta ttatctcatg gattttttca tgcagaccca catccaggta atatattcgt
     6181 agttgctaaa aataagatag catatataga ttttggaatg attggaatta ttgataataa
     6241 aactttaaac caattaaatg aaattgcatt agcaggtgta gaaaagaatg ttgacaagat
     6301 aatatatcta ttgatagaaa tggatgcatt aaatggagag gctgatatta agggacttag
     6361 acaggattta ctttatctaa tacattatta ctatgatatt tctatagaaa aaataaatgt
     6421 aacagatata ttaaatgaac tatttaggtt ttttagacag tataaaatag tcatgcctgc
     6481 acaatttgta actcttgcaa agacagttat aacattagaa ggaacaagta gaactttaaa
     6541 cacagatttt tcttttggtt ctatgggaaa agagtttatg aaacatcatt ataagagtaa
     6601 atttaaccct aaaaatgttg ttttaagttc aagacaaaat gtagaagaaa tattactgga
     6661 cataaaaact attccaaagc aattaaaagc aattttaaaa aatatagaaa gaaataatat
     6721 aaaaatgcaa attgaagatg ttaaaatgac gaggttagaa aattgtataa tagagctaac
     6781 atctcaaata tctttgagtt tagtgttggc atctattata gtaggttctt ctcttataat
     6841 tgcatctcca aatatagaaa ataatatatg gataaaattt actgcaatag cgggtttctt
     6901 tatatccttt ataataggcc tttgcttagt tataagaagt attaggtcaa aatacaaaaa
     6961 agattagggg gaattaaatt tgaaaaaatg gatattgaag tataaaggta agatagagga
     7021 aagtgaattt agcaaaaaac ttaatatttc cccggaaatc tgccaaattt taaaaaatag
     7081 agggatatgt actgaaaaag attcagaaat atttatgaat ccatctttag attatttaag
     7141 agaccctttt ttaatgaaag atatgcaaaa agctgttgat agaataaagt tagcagttga
     7201 gaagaacgaa aaaatttgga tttacggaga ttatgatgtt gatggtgtat catctacatc
     7261 tatcttgtgc ttatatttta agagtatagg ttacaatgtt gattactata ttcctaatag
     7321 attagaagaa gggtatggta taaatgaaga tgctattaag cttataagct ctaggggatg
     7381 tgacttgatt ataagtgttg actgtggaat tacatcagtg tctgaggtca atacagcgaa
     7441 tgatttgggc atagatgtta taattacaga ccatcatgaa tgtcaaagtg aaattccatc
     7501 agcctttgca gtaataaatc caaaacaaga agattgtaat tatccatttg attcactatg
     7561 tggatgtggt gtagcattta aaatgattca agccttgact ccaaaagaag aatttaaaac
     7621 tagtatgtat aattatttgg aaatagtaac actagcaact atttgtgata ttgttccact
     7681 tatagatgaa aatagaataa ttgtaaaaaa tggtttaaaa tcaatgaaag aaggcaaaaa
     7741 tattggatta agggagctta taaaagtttg tggtgtagaa agtgataaga taggttcttc
     7801 tcatataggt tttgcaatag gtcctaggat aaatgcttca ggtcgattgg gatattctta
     7861 cttaggagta gagctattta ctactcaatc acaagaagag gcagtagaaa tagctagtat
     7921 attggaagaa aaaaataatg aaagacagat gatagaagct aagatgtatc atgaagctga
     7981 agaaatgcta aagtctaata gcagatacaa tgatgacaag gtgttggttt tagctaaaga
     8041 gggttggcaa catggaataa taggaattgt agcatcaaag ttaactgaaa aatactacaa
     8101 accaacaatt cttttaggta tagaaaatgg ggaggctact ggttcagcga ggtcaataaa
     8161 aggatttaat atatttgaag ctctcataaa gtgcaaagac ttgatgacta agtttggtgg
     8221 tcatgaacaa gcagcagggt tatccttgga ttcagacaat gtagaaattt tagcaaatga
     8281 aataaataaa tttgcagatt ataatttaac tgaagatgat atgatagaga atgtaaatgt
     8341 cgagtttgaa ttacaagaaa atgtaataaa tttaaatctt gtagaagaat tacataagtt
     8401 agaacccttt ggattaaata atcctaatcc tagatttata gttagaaatt atattttaaa
     8461 agatttgaaa gttataggta agaatcaaca acatctgaaa ttaagcatag aaaaagagaa
     8521 aagctatgaa tgtataggat ttaatatgag tcatttaaaa tcaatgtata aagtaggaga
     8581 caaggtagat gtactattcc aattagatga aaataactat atgggaaata ggaaagtaca
     8641 atttttactt aaagatatac gattggctcg acctaaaagt gcaagtaatg acaaactttc
     8701 attgaaatta atgtcaaaaa taataccaaa agatactcaa agcctgtata atatatctgt
     8761 gtctgatttt gaattatttg atggaaatac agatataaat atatttgatt attttgaaaa
     8821 agatacacta ataatatcta attctataaa tggattttat agagcaatgt cagatatatc
     8881 acttatagat ttggatttta acataaatta caatattatt gaagatgtta gcaaaaatac
     8941 tgataaatta gagcttatat tttctcctaa tattgataaa atagacttaa aaagatataa
     9001 taatattatt ctttatgatt atttatacaa taaaggcgaa tattcgtata tatatgagaa
     9061 taaaagagaa gaaagtgaaa ttataaaata ttataataaa acagacctat tatatttaaa
     9121 gaatgtagtt agtaatatag ttccatcaag agatgaattt ataacaattt ataaacaagc
     9181 actcataaaa aaagaaattg atttggatat ggtaaatata agggaaactt ttaatgtaat
     9241 tccattaaag ttttttacga tattaaatgt atttagagaa ttaaatttac ttgactttaa
     9301 tttaaattat gaaaaaaatt ctgtactcat aagaatattg ccaaaaccac agaaaaaatt
     9361 agacttaaat gagagtttaa tactcaataa tctaaaaaat ttagaaaaac aatacaatag
     9421 cagttattag gaggcgtttc ccatggattt aaaaaacttt ataaggaata ttgatgattt
     9481 cccaaagcca ggcatagatt ttaaagatgt gacaactcta tttaaagatg gagatgcatt
     9541 taaatatgca gtggattcta tagttgaaga attgaaagat aaagatgtag atttagtaat
     9601 aggaccagaa gcaagaggat ttttgatggg tacacctgta gcatatgctt taggagtagg
     9661 atttgttcca attagaaaac ctggaaagtt accaggtgaa gttgagagtt atgagtatgg
     9721 attagagtat ggtacagata ctttagagat acataaggat gcaattaaaa agggtcaaaa
     9781 agtagcaata gtagatgatt tattggctac tggtggaact atggaagccg ctgccaagct
     9841 tgtagaaaaa ttaggtggag aagtagtttc tatgcaattt ttgattgagc taaaattctt
     9901 aaatgggaga gaaaagttgt ctaactatga tgttaattct cttataaaat actagtactt
     9961 ttataatagt tggtataaca ccaactattt tcatatatat taaaatttta tacaaaatag
    10021 gtggttatat gcatgataaa gaattacaag aaataattga aaaaataaaa gtttatgcac
    10081 ctaatgcaaa ttttgaaatg gttgaaaaag cctataatct tgcaaaaaaa gcgcatgaag
    10141 gacaatatag aaaatctggg gaaccatact ttattcatcc attagcagtt gcaaatattt
    10201 taatagagat ggagttagat attgaaacta taacagcagg tttacttcat gatgttgtag
    10261 aagatacaga atatacctat gaagacataa aaaaagaatt tggcaaagaa gtagcggatt
    10321 tggttgatgg agtaactaaa cttggtcaaa taaaatatcg ttcgaaagaa gaaactcaat
    10381 ctgaaaattt aagaaaaatg tttttggcaa tggcgaagga tataagggtt atacttataa
    10441 aacttgctga tagattgcac aacatgagaa cattaaaata catgcctcca gaaaaagcaa
    10501 agtataaagc aaaagagaca cttgaaattt atggtggtat agctcataga ttaggaattt
    10561 ccaagataaa gtgggaacta gaagacagag cattaagatt tatggaccca gaagggtatt
    10621 atgatttagt tagtagagta tcaatgaaga gaagtcaaag agaggattat attcaaggaa
    10681 ttgttgaact tcttaaagat aagtttaaag atttaagtat accatgtgat gtatatggta
    10741 gaccaaagca tttttacagt atatatagaa agatgcaaaa aaaacataag acttttgaag
    10801 agatttttga tttaacagca gtaaggatat tggttgataa tgtaaaggat tgctatgcag
    10861 tacttggtat agttcataca ttatggagac ctattccagg aagatttaaa gattatatag
    10921 caatgccaaa acccaatatg tatcaatcat tacacaccac tgttgtaggt cctgatggag
    10981 aacctttaga gatacaaata agaactcatg agatgcataa tatagctgaa aatggtatag
    11041 cagcccattg gaaatataaa gaaggtatct ctagcaaaga gtcaaaggtt gaagaaaaac
    11101 ttcaatggtt gagacagatg atggagtggg aaaaagattt aaaagaccca caagagttta
    11161 tggatgcact taaagaagat gtatttaata gtcaagttta tgtatttacg ccaaaaggag
    11221 acgtaattga attaccagca gaatcaacac ctatagattt tgcctatagg gttcatacaa
    11281 atgtaggtaa taaatgtgta ggagctaaaa ttgatggaag aatagttcct attgattata
    11341 agcttcaaaa tggtaatatt gtcgaggtac ttacatcatc taattctaat ggaccaagta
    11401 gggattggct taatatagtg aagaccccaa atgcaaaaag tagaattaga caatttttca
    11461 agaaggaaag aagagaagaa aatgttgacc gtggaaatac aatattagaa agagaattta
    11521 agaagcatga tataccacaa aaagaccctc ttgttgaaaa atatatggca ttagttgcta
    11581 agaaatttaa tcagccaagt gttgaggact taattgccac tgtaggttat ggtggtataa
    11641 tgtcatctca agtggtatct aaggtaaaag acctatattt aaaagaggtt aaaaaagcta
    11701 aaaaggaaca acagatacaa gaagaagatt taaataaaca caatataagt gaaacagaat
    11761 acagtaaaaa gagaaagaaa aacgcatcac aaggaattat agtaaaaggg cttgacaata
    11821 tcttagtaag atttgctaag tgttgtaatc ctcttccagg agatgaaatt gtaggatata
    11881 ttactaaggg taggggagta gccatacaca gagctgattg cccaaatgca aacaataatg
    11941 gtgatttctt ccaaaataga ttggtggagg tttcatggag ttcagcagga aaaggtaagt
    12001 ttgaagcgga aattcaaata aaagctattg atagaagagg aattgttaat gaaataactc
    12061 atattgttgc aatggataaa cttagtttaa acggaataaa tgcaagaaaa ggtaaggata
    12121 gtattgtaag tataaactta cttgtagagg ttaatgacac agaggaatta aaaattctaa
    12181 tgaaaaaatt aaaagcaatt cctggtgtag aaagtattta tagaatgaca aattagagtt
    12241 aaaataatag ggaggaaact atgagagcag tagtgcaaag agtatcttca tctaaagtta
    12301 cagtagatga aaacactata ggtcaaataa ataaggggtt actagtattg ttaggagtga
    12361 ctcatgatga taaatcaagt gatgtagatt atatgattga taaaatattg aatctgagaa
    12421 tttttgaaga tgaaaatgac aagatgaatt tatctctaat ggatataggt ggagagcttt
    12481 tagtagtatc tcaatttact ttatatggag attgcagaaa aggaagaaga cctggattta
    12541 gtaatgcagc tagaccagaa cttgcaaata acctttatga agaatttgta aaaaaagcaa
    12601 aagataaagg tgtgacagtt ggtacaggtc aatttgcagc tcatatgatg gtagagttaa
    12661 ctaatgatgg tccagtaaca atactattag actcaagtaa atctttttaa gggggaaaac
    12721 tttaatgatt ataaaaaaat ttgtagatag ttattttgga gtaaatactt atgtattagg
    12781 tgatgaaaag actaaaaaat gtgcagttat agacccaggt ggaagtttag tagagacatt
    12841 aagttttgta aaagaaaatg aattaaatat tgaatatatt atccttactc atgggcatgg
    12901 agaccatata ggatatgtta aggatattaa agaaaaaact ggtgctaaag tagttgcaca
    12961 tgttgatgaa aaagaattat taaatgataa aaataaaaat ctaagttata caatgagatg
    13021 tggagcgcaa gagtttgatg cagatatcta tgtgaatgat aaagacaagc ttgaacttgg
    13081 tgaattaaaa atgactttct tgcatactcc aggacatact caaggatgta tgtgtataag
    13141 agttgaagat gaaatgttca caggagatac tttgtttgct ggaagtattg gtagaactga
    13201 tttatatagt ggggatttta accaaatgga aaaatcactt aagaaattat gcaaatatga
    13261 agatgctgtt agagtatatc ctggacatgg accaaccagt actctagggg ttgaaaagaa
    13321 gacaaatcct tacatgtaat tttatattag tatttaaaaa agtataaaat atttaataac
    13381 tatattagtg agggggttct tctactgtta aatagaagga gttaagttga tgttgtatgt
    13441 ttttttaaaa ggacatgatt ataagtatga agtagcagaa cttataaaat tgtttaccag
    13501 tgaatttaag tttaaagact ctaatgctaa caataatata gagtcaaagc atttaataaa
    13561 tagattgatt tatgagaatg gtgtattatt ctcaagtact gaatactatg aaaatggaaa
    13621 cttaaagtat gagtcaatac agaatataaa aaatatgaat cttgaatttt tagatggatt
    13681 tgatagtagc aaaacgattg aagaatttct taatagccta gatagtgaaa gtaagaggaa
    13741 ctttaaaaag ctgtgtaaag aaaatatcaa aaaaagcatg tttttagtgc ttaaacaagt
    13801 atttaattct tatgtaccat ggggaatttt gactggtatt agaccagtca aaattgtcca
    13861 caatttaatg gataaaaagc tagatgataa tagcataaga accattctaa aagataatta
    13921 ttttataata gatgagaaaa tagatttagc tcttgaaata gctaaaagag agcgattatt
    13981 tatgtatcct atagataaaa ataaaatttc tctttatgta agtattccat tttgtccaac
    14041 tagatgtgta tattgttcat ttccttcaaa ttcattaaaa caatttggac atttaaaaag
    14101 agaatatgtt tataaattga ttgaagaaat taaggggttt gccaaagtta ttaaggagac
    14161 taagaaagaa gttgaaactc tatatattgg tggtggaact ccaactacac ttgatgagga
    14221 agaattggat ttgcttataa attcattgtt caatgaatta gatttaagta aaattaaaga
    14281 atttactgta gaagcaggta gacctgacac tattacagaa caaaaactaa gagtgttaaa
    14341 aaaacacaat gttagtagaa ttagtataaa tcctcaaact atgaatgatg atactttagt
    14401 aaagattgga agagagcata gagtatctga tttagtagat tgctttaata tggctcgtag
    14461 aatgggattt gataatataa atatggatat aattttaggt cttatagatg aagatttaaa
    14521 tatggtaaag aatacattgg aggagataaa aaaactttct cctgaaagcc ttacagttca
    14581 tacattagcc ataaagagag catccaactt aaatataaat atggataggt ataaagaaca
    14641 tctaacacaa tatgaagaaa tggtaaaaat gatagattta tcaatgaaat atgcaaaaga
    14701 tatgggatta aatccatatt atatgtacag acaaaaacat atgttgggaa atttagaaaa
    14761 tataggttat gctaaagaag gatatgagtg catttataat atacagatta tggaagaaaa
    14821 acaaagtaac tatgcattag gtgcaggtgc tatatcgaaa tttgtttatg tagatgaaga
    14881 tagaattgaa agagtagaaa atgtaaaaaa tgttgaacaa tacattgaaa gagtagatga
    14941 aatgataaaa aggaagaaag aagaggtata taaaaatgtt gactaaagct cctagaggaa
    15001 ctaaagacat aactccaaaa gaggcttaca aatggcgtta tgtagaaaat aaatttagag
    15061 aaatatgtgc tttatatgga tatgaagaaa tggtaacacc tatatttgaa catacagaac
    15121 ttttcaagag aagtgttgga gatactacag atatagttca aaaagaaatg tattctttta
    15181 aagataaagg tgatagagaa attaccctaa agccagaagg tacagcaggt gtagtaagag
    15241 catttataga aaataaatta tatgcagata ctcaaccaac aaaattattt tatgtgacac
    15301 cttgttttag atatgaaaga ccacaagctg gtagacaaag acaatttcac caatttggaa
    15361 tagaagcact tggaagtgat actccttcaa tggatgctga aataatcgca ctagctgttc
    15421 aattttttaa tgaagttgga ttaaatgatt tggtagtgag tataaattca gttggctgtc
    15481 cagtttgtag aaaagaatac aatgctttat taaaagagta tcttgactca aaagctgata
    15541 tattgtgtga tacttgcaat gagagaagag aaaaaaatcc aatgagggtt atagactgta
    15601 aaaatcctac atgtaaagaa aatatcaaag atataccatt tattgcagac catttgtgtg
    15661 atgattgtaa atctcacttt gataaacttc aagaatactt aaaggaaatg aatataaact
    15721 ttgtaattga taaaacaata gttagaggct tagattacta tagaaaaact gcttttgaaa
    15781 ttatatctaa tgatatagga gctcaaagta ctgtatgtgg tggaggaaga tatgatggtc
    15841 ttgttgaaca attaggagga ccaaaaggta taagtggaat tgggtttgga ctaggaattg
    15901 aaagactttt acttacactt gaaggtaatg gaattgaaat agaaaaccct caatctacgg
    15961 atatatttat agtaactata ggtgaagaag caaatacaag aagctttaaa ttgttaaaag
    16021 acttgagaca aaatcatata agtgcagata aagaccatat agagagaagt gttaaagcac
    16081 aatttaaata ttcagataaa ataaattcaa aatttacaat agtaattgga gatgatgaac
    16141 ttaaaaatga cactgcgaca ttaaaaaata tgaaaacatc agagcaaact actgtaaaat
    16201 taagtacatt agttgaagaa ttaaagcaaa agctgtaaaa taagaaagta tgatgttttg
    16261 tgtattagat aaatatatag cattaaaatt aaataaaatg gaggtagtat cttggaaact
    16321 ttaaagggtt taaagagaac tcactactgt ggagagttga gagaaaaaaa cataaatgaa
    16381 gaagttgtac ttatgggatg ggtacagaaa aaaagaaatt taggtggtct tgtatttgtt
    16441 gatttaagag atacaagtgg attatgtcaa atagtttttg atacagatgt agataaagaa
    16501 gcttttgaaa aagctgaaaa attaggggct gaatttgtaa tagctgtaaa aggaaaagta
    16561 tgcgaaagac aatctaaaaa ccctaatatg ccaactggtg atatagaaat atttgcaact
    16621 gaacttagac ttttaaacaa gtcagaaact ccgcctattt atataaaaga tgatgatgat
    16681 gtttctgaag cactaagatt aaaatataga tacttagatt taagaaaacc atctatgcaa
    16741 agaaatttaa aattaagaca taaagtaatg aatattacaa gaaattactt atcaaacaat
    16801 agattttgcg aaatagaaac accattttta atagctccaa caccagaagg agcaagagat
    16861 tatctagtac caagtagagt taatccagga aaattttacg cattaccaca atcaccacaa
    16921 ttatataaac aattattgat ggtaagtggt atggatagat atttccaaat agttaaatgc
    16981 tttagagatg aagacttaag agcagataga caaccagagt ttacacaaat agactgcgaa
    17041 atgtcttttg ttgagcaaga agatgttatg agtatgattg aaggtttgct tgaagcgata
    17101 tttaaagaag ttttagatgt agaattggca cttccacttc ctaagatgac ttatgcagaa
    17161 gctatgtcta aatatggttc agataaacca gatactagat ttggatatga gttaacagat
    17221 atatctgatg ttgtttgtaa ctgtggattt aaagtgtttg ctgatgctac acaaccagga
    17281 aaaagtgtta gaggtataaa cgtaaaaggc aaagctgacg actttactag aaaacaaata
    17341 tcatcactag aagaacatgc taagacatat agagcaaaag gtcttgcttg gatgaaagta
    17401 ggacaagaag gagtaacttc tccaatagca aaattcttta atgaagaaga aatgaacgct
    17461 atacttacaa gaatgaatgc tgaagtagga gatttacttt tatttgtagc tgataaaaac
    17521 tcaatagtat ttgatgcttt aggacaagtt agacttgaag tagcaaatag gcttaatcta
    17581 ttggataaaa atgtatataa cttactatgg gtaactgagt tcccagtttt tgaagaagat
    17641 gaagaaacag gcacattctc agcaatgcat cacccattta catcacctat ggatgaagat
    17701 ttagacaagt tagaagaagg tgataagtca tctttaagag cgaaagctta tgatattgta
    17761 ttgaatggtt atgaaatagg tggaggaagt gttagaatat caaattctga tgttcaatca
    17821 agaatgttta aagcacttgg ttttacagag gaaagagcta atgaaaagtt tggttattta
    17881 ttagaagcat ttaaatatgg tactcctcct catgctggtc tagcttttgg tcttgataga
    17941 cttgttatgt tacttgcagg aactgataat ataagagaag ttattgcatt ccctaaaaat
    18001 caaaatgctg tttgccctat gacaaatgct cctacattgg cggaagatga gcaattagaa
    18061 gaattaagta taaaagttga tataaaagat aatgaataat atgtagttgc aagaaactaa
    18121 aatgtaaaaa gtctggatat aaagtatatt tatattcaga ctttttttat tagaattatt
    18181 ttatattttt tctctatata ttttcataaa ttatacttgt attctcaacc aatataatta
    18241 aaatactatg tctaaaaatt ttacataaac ttggttgttt tagaaactac ttctgtaatg
    18301 agtacacgat tttaattaaa gaatatataa caaatttgaa ataagtctaa tatatgactt
    18361 aatagattaa attaaaataa taaatttgga ctatttatct atttataact taaattatat
    18421 caaattgata acaaactaga tattatactt ttttgtagaa tagaattaca attatataaa
    18481 gaaaaattct attaatagtg aaaaaaaagt agaaaaaagt agaaattatt cttttataaa
    18541 gaaagggagg gataatattg caagagagag aagaattagt tttaaatcca gttccaaaag
    18601 aggaaagagt tggatggtta gctccattgt ttaatatgct tggttctaac atagctatat
    18661 ctgaactaat ggttggggga actttaattt taggaatgac tctttctaat atgattatta
    18721 catcaattat agggaactta atattagttg caattattat gatacaaggt tatataggtt
    18781 acaaagaagg gttaaatacg tatgtattag ctaagggggc atttggagag ataggaggaa
    18841 agtatcttat atctctatta cttggtataa ctagttttgg atggtttggt gtacaagcag
    18901 gggttgcagg actatcagta caaaagatat tcccaagtgt aaacctaaca ttagttacag
    18961 ttatacttgg attatttatg gttgtttttg cactgtatgg ttttaaagct atggctaaat
    19021 ttaattactt agttatacca ccacttatga tattaatggt atggggagtt tttaaagctt
    19081 tttctactta tggagttgaa gctatttata attatactcc tcaaacaact atgagtatgg
    19141 ttgaaggtct taatattgta gtaggtcttg ttatagttgg tgcaattata tcacctgacc
    19201 agttaagata tacgagacga gtaaaagata tttggattat cagtttttta ggtctaggga
    19261 ttatttcatt attccaacaa gttgcagctg gtgtaatgtc tatgggagca cctacttggg
    19321 atataacaga agtattggct aatttaggtt ttggatgggt agcgtttgtt attttaatac
    19381 ttgcatcttg gtcaacaaac gtatcaaatg cttattctgg tggattagca ttaaaaacta
    19441 tttttccaaa tgtaaaaaga aatgttttga ctttagttgc aggtctaata ggtacaataa
    19501 tagcagctac tggtataata tttaaatttc agtcattttt atctttcttg ggaatcgcag
    19561 ttcctgcaat agcaggaatt atgtggtgtg agtattattt tatacaagga agaacttata
    19621 agcatagaga aggaataaat tggattgcag taataagctg gttaataggt tttgcagcgt
    19681 cgtattatag ttcaaaaata aatttcttaa tacctccgat aaatggaatt gtagtgagta
    19741 tggttattta ttatatatta atgaaatgtt ttggaatcaa agataaataa agggggcaat
    19801 tacatgggtc gtaaggtgaa aataggtata attcaacaac acagtgtact tggagatgtt
    19861 aaaaaaaata tagaaaaggc agtagagatg atagatgact taggaaaaca aggggcagat
    19921 ataatttgtt taccagaact atttgctaca ggatataatt tagagtcttt aggtggagtt
    19981 aaaacattag aattaataag agaacataat aagtacatag aggaatcaat gtcagaagct
    20041 gctaaaagaa ataatgtata cctgatatct ccatatggaa ccttagaaaa agggtctaca
    20101 catgtttata attcagcagt tatatttgat agaaaaggta agattatggg agaatactgt
    20161 aaaaatcatc tgtggtcatt agaagcagta tactttaaag gaggggaaaa agtagaggta
    20221 tatgatgctg attttggtag atttggtgta atgatatgtt atgatgcagg ttttccagaa
    20281 gtatccagag aattgacatt aaaaggttct gaaataatat ttataccatc tgcatggaga
    20341 atacaagatg aagatatgtg ggatttaaat gtatctcaaa gagctttaga gaacactgta
    20401 tatacagtag gtgtaaattt agtaagtaat gactctaatt taatactatt tggaaagagt
    20461 aaaatatgca atcctagagg tactgtaata actcaattag atacatacag ggaaaaatat
    20521 gtattatcag agattgattt agatgaaata aaaaagtata gggaagatat cccctatttg
    20581 aaagacagaa aaagtagcta taacatataa ttctatacta taatacaagt tcaatataaa
    20641 atgataaaaa gaggatgtat caatattaag attaaattta ttatactttg atatatcctc
    20701 tttaatatta tcaactttga tttaaatatt tttaaaatag ttgataagaa agtttttgaa
    20761 caacaacaca gctttagatt catagttttc gctaaaccat gaaagattta aatatctttt
    20821 aaattcaata tctttgatat gtaacaactt aatctcaggg tcttgagata atccccatga
    20881 ttttccacat ataaatccaa ctccttgaag tgattttata agagcataaa ttgtatatgg
    20941 tgaatcagac tcaaatgcta ttttgggttt gaaattagct gattcacaca ggatatcaat
    21001 tacctctcta taattctcac ccttcgttat aactataaag ttttcatctg aaacttcgct
    21061 tagatatact tcatccttta atgataaagg atgatttatt gaaactccta atagtatttc
    21121 ttcgcatgtt aaagttatgc tattttctga gtttagcttt gtaaatgaag aagatatgta
    21181 caaatcaaaa tcactttttt tataagatga tggcaaagta tgtgacacat taaaagtaat
    21241 attgggatat agttttctga aattactcaa aagtgatggt aacacattag ccgctgcagg
    21301 agataatatc tttacttctc ctgaattttc taaagaagtg tcttgtaact ccttttttga
    21361 actttctact aaatctaaaa tgctatctac tctttttaga aacaatcttc catactcatt
    21421 taaaacaata tatcttcctt ttctgtcaaa taactgaact ccaagttctt tttctaagga
    21481 agatatagtt ttgcttaaag ctggctgtgc tatatttaac ttgtgtgatg cttttgttat
    21541 gtgctccatt ctagcaacag tttgaaaata cttaagatgc aataaatcca tagtataaaa
    21601 tacacctctt ttaatatgac ttgagttata tcaattttat aatcaactcc acattataca
    21661 ttataatata aagtttatat aataaactac aatatattaa aaagttattt aaattgttat
    21721 ttatattttg tacataatct aattaaaaaa agtaacttta aatgattaac taaggaaatg
    21781 ttgttacaaa ctttagttat ataaaaaatt catttgatga gcaaagtatt aatgtcaagt
    21841 aagaataaaa gcttattaga taagaaaaat aatattttat aataatgatg ttacttaaat
    21901 tattatgtta tactatttta aataggaaac atttagatac tataatttta gggggagtca
    21961 agatgaaatt aactaaatca atatcatcaa tgctgtgctt atctctagtt ttaagcttat
    22021 caccagtagc atatgcaaat gaaataagta gaacaacagt tgagaatact ttagtaggtc
    22081 aggatagata tcaaactgct gttgaagtaa gtaaaaaagg atggagtagt gcaaatgagg
    22141 ctgtagttgt aaatagtgaa tctattgtag atgcattagc tgtaacacca tttgctaaat
    22201 taaaaaatgc tcctatatta ttgacagaaa aaaatgatct aaattcacaa actgagaaag
    22261 aattaaaaag attaggcgtt aaaaaagttt atataattgg tctatctagt tctatatcaa
    22321 atgatgtgca aaaacagtta gaatctaata atttaactac tgatagagtt ggtggtagtg
    22381 atagatacca aacagctttg agtatagcta aaaaaataga aggattaaaa gatatatcag
    22441 aaatagctgt agtaaatggg tacacaggtc ttgcagatgc agttagtata gcttctgtag
    22501 ctgctactaa tggaatggtt atacttccag tatctgatgc atctggtata tctagcttta
    22561 aagattttat agaatctaag aatataaata aatcatacat aataggctct actaatgctg
    22621 tatcagataa aataaaacaa agtttaccta attcagaaag aataggtgga gcagatagaa
    22681 atgaaactaa tggtaaagta atagaaaagt tttatacatc taataaatta aacaatgtat
    22741 ttgtggctaa ggatggaatg aaaaatcaaa atcaacttat agacgctcta gcagtaggag
    22801 tactagcatc aaaagaaaat tctcctgtac taatcgtagg agaacaatta agtgcaacac
    22861 aaaagagtgt attttcgaat aaaaatgcta cctctataac tcaagttggt agtggaggaa
    22921 atgaaaatgc atttaaagaa ttaaaagatt tacaggcaaa tagtaacaat aagagagtaa
    22981 tgtatgtaac taatacagat aaggtaaata taagaaatga tgctacaata gaagcttctg
    23041 ttataggata tctaaataat ggtgatgaag tagaagtttt agatgtgtta aaaactggat
    23101 gggtaaaaat aaaatacaat gaaggtattg gatatgttag tgggtcttat ttgacaaata
    23161 ataagcctga taatagtaat gaaaatataa aaataaagta tgtaaaagaa aaagatggtc
    23221 taaatgttag gaaaggacca agtacagaag atgaaaagat aggtcacctt tcatatggaa
    23281 gcaaagtaga aacaatagaa atgtttgcta ctggatgggt aaaaataaag tataatggtg
    23341 gatatggtta tgtaagtaat gattacttat ctgatacacc agttatttaa ttataagtaa
    23401 caataaaaaa ctgctttaat ttgtaaagca gtttttttat aaaaatttaa gtctaataac
    23461 tatttctatt ttgatacctt aatcctggga tttattatta tggatattat caacatacaa
    23521 acactccaaa tccagtgtct aattccaaac ttagatatta ataaattcag tcctgctatg
    23581 gtaaataagc agtatttaat tacaattaaa agtgtattta tagaaagccc cttcatagaa
    23641 agtatcctaa atccaataat agacattcta agtcctataa atgccaataa tatacctgca
    23701 atatcctgaa ttaagtaata gtattcaaat ttcatattca atcaactctt ctctgagcag
    23761 tatcaaatat ttctaactct tcaatttcat catctgtgta tgtaagcata gtaaaaccag
    23821 ttacaccata aataaatgaa attataggtg taatccaact taagaaacaa tatggaataa
    23881 attctagtgg gcttacacca agggtctgag cacagaaaac agcatttgaa ttccatggta
    23941 ttaatggacc accaagtgta gctgtatctt ctataattct agatagattt tgtggtttta
    24001 atttaaattc tttgtatatt ggagacatta gagttccgac aaatacagaa gtgaacatca
    24061 ttgatgaact aaatgcattt gcaaagtagc ttactacaaa agtagaccca accaatttag
    24121 ttcttgattt tcctatttta gttataagag gttgaagcag tacttctaag aatccagttt
    24181 tttggagaat accagcaata acgaaaacta caaatactag taaaacggtt tcagccatac
    24241 tcattattcc accacgattt aatagtttgt cggcatagac aaatccagta tctatagtaa
    24301 atcctgatag catgtaattt aaaacatcac ctattttttc tccttcttga aatactgcta
    24361 taataagtcc catgatagct gaacttagta tagatataat gggtggcttt tgtagcaata
    24421 aaagtaataa taaaaatatt attggaatca ttgctactaa accaatatga aagtttgaat
    24481 ttaatacatc cttgatttga tttatttgaa catagtctat agtattattt gaatacttaa
    24541 atccaattac tgtatacaga actatacata ttacgtatgc aggtcctgtg gtataaaaca
    24601 tatgcttcat atgtgtaatt acatctgtct tacaaactgc tgcagctaag tttgtagaat
    24661 ctgatagagg tgacatctta tctccaaaga aagcaccaga aatgactgca ccagctgtta
    24721 aaccaagagg aatacccata ctagtaccaa tactcatcat tgctatacca gaagttccaa
    24781 gagtccccca agatgtacca gtagcaacag aagttaatga acatacgatt aatgttgtaa
    24841 gcagaaaata tttaggggtt attattgtta gtccactata tactactgtt ggtattgtac
    24901 cagcagctat ccaagctgct attagtacac caacagaaag tataattaag tttgattgaa
    24961 aggcatcttt accaacttca aatccaaaag cttctatttc atcattagta taaccaagtt
    25021 tcatggctgc aggaactact ataagccatg aaagtaaaaa cattgtagtt atagaagcat
    25081 taaatacaac tataccaact gatgttagag ctattattgc aagcattact aacaaggaat
    25141 atctaaatga aggtattttt ctttctgtat tcattttcat agtgcaaatt ctccttaaat
    25201 atttttagtt aattgcattt tttattctta taagagcttc ttcaagcata cttcttggac
    25261 aagctaagtt tattctttga tacccacttc ctccaatgcc aaagctgttt ccttgattta
    25321 gagcaacttt acctttttgt acaagtatac tttctagttc ctcatcactt aatccaagtg
    25381 cactaaagtc tacccataat aaataagtac cttctggttt tctaacttta agttttggca
    25441 tattttcatt tatatatttt atagcaaaat ctatattaga ttctaaatat tctaaaaatg
    25501 attctagcca agattcacca ttattatatg aagcttcagt tgcaactaag ctaaagcaat
    25561 tatttctctt tatatcaatt ctggtaaagg catcatctaa caatttatag tctttttcat
    25621 caggtagtat tacatatgat gattgaagac cagctatatt aaaagtttta gttggagcca
    25681 tacaagtaat tgtatttttt tcaaattctt tagaaataga tgccatagga atatgcttat
    25741 gtttttttaa tataatgtca ctatgtattt catcagatat gatttttaca ttatgcttaa
    25801 gacaaatatc tcctagtttt tttaattcat cttttgtcca tactctacca actggattat
    25861 gaggattaca aagtatgaat aattttacat ctttaatctt gttttcaatg tcttcataat
    25921 ccattatgta gtttccgttt tctagttttt gtaatgggct tataattagt tctctattgt
    25981 tattttttac aacactatta aaagggctgt aaactggttc ttgtatcatt attttatcat
    26041 ttgctttcgt gagttcattt attaaaagac ttatagctgg gataacacct ggactataaa
    26101 ttaaccactc actttttatt ttccagttat gtctcctatc tagccagttt actatagatt
    26161 cattgtaaga atctggtctt gttgtgtagc cataaatctc ttgttctagt ctatttttta
    26221 aggaatcaat tatacaaggt gcagccttaa aatccatgtc agccacccac ataggaagta
    26281 aatcattagt tccatacttt ttttccattt ctgaccattt agatgaaaaa ttattgcttc
    26341 tatcaacaat ctcattaaaa ttataattca ttgtaaatac ctccagtaat tttaatttta
    26401 tgttaataat attaagcaaa caacgtgcca atattgataa taaaaaatat ttattcacaa
    26461 gttaatattt aatattaagt aatttcagag catagataag ataaaaatat taattatact
    26521 taaaactttg tggaaaaggt tatttctaat aaatttttaa atatatgaaa atgtaattaa
    26581 ataggttaaa taggtttaac tgtttaactt gttagaccta tttatatttt ttagaaattc
    26641 tactcctttt gatgttataa ttgaacctcc acgaccaata agtatgtcta caagcccaaa
    26701 gtcttgtagt tctaaaagca tatttctaat ctgttgttct gtcaaaaatc tattttcctc
    26761 taaagctatt ttacataagc tttttctacc tattctttct tttaaatcat aagccttttt
    26821 taaattatcc aatataaata tgtattcatc tagtgacttt ttaaaattat agtcgtattc
    26881 catgatgttt ttgtctttgc caatgttgaa ttgatacttt tttatattat cacttacttt
    26941 attacaaacg gttctactat aatttgaatc tatagtatcc aatatatatt caggtaaatc
    27001 acatatttct ataatatctt tttccaagta acagaagtat tctcctaagt ttcttaattc
    27061 tcttacattt ccttcccatc tatacatttt aaagatttct tttacttcct cacttagaat
    27121 aaaattacat ggaatatcat attttaatga accaaaaatt tcaaatatat cagcaggtct
    27181 ttccctaagt ggatgtattt taaggggaag aacattcaac ctaaaatata aatctcttct
    27241 aaacatattt ttagatacaa gttcttttaa atttctatta gtagctgcaa ttattcttac
    27301 atcaatgtca atgacattat tagaaccaat acgtcgtact tgtttttctt gtatgactct
    27361 aagtaattta gattgagaat ttaaatccat ttctccaatt tcgtctaaaa atatagttcc
    27421 attgttggct aattcaaata aaccaatttt cccacctttt ttagcaccag taaaagcccc
    27481 ttcatcatag ccaaataatt cactttgcag tagattttct tgaaaagtac tacaatttac
    27541 tgcgacaaaa ggaccatctt ttctcctaga agcactatga atggattgag caaataattc
    27601 tttaccagta ccactttcac caattataag gatagatgaa tttgattgag ccattttatt
    27661 agcaattttt tttgtattaa tagtttgtat actagaacct aatatgtcat caaatgtgta
    27721 tttactgata ttaccagagt ttactagttg tgctcttaat ttagcttgct ttttttctaa
    27781 ttgagaaaac tttgtcatct ttagtataaa tccatcaaat acatttaatt gtatatgttt
    27841 aatctctaaa tttatatcaa tatgatttat tttaatcaat ttctcaaaaa atggagtttt
    27901 attggtcaat atgctttgaa aattgatgtc ttttatgcaa tcaccaacaa atgagtcaat
    27961 catttcattt gcatttatag acaaaatttt tctagccata tgattataga attgaattat
    28021 tccatcgtta tttgtgcata taattccatc atcaattata gatagtaaca aatcaaattg
    28081 tctttcaaac ttatttgcat ctaacaataa ctcacctgta ctataacttg taggaattat
    28141 tttttctacg tattttttta ttaggtcatc ttttattaga tgttttaatt tggtttttgt
    28201 agcaatttca acaatacaac ttaaatctat tattctgtat tttaaatcta taacttcaca
    28261 atttttaaac ttaggcaaga ttttttcgcc aggagttagg attacagcat ctgtgggagt
    28321 aaactcaatc tctggatagc agggcaggat atttatattg tctattccta atctatgtat
    28381 taaagaaatt gtttctattg ccatatcttt acttaagtta tatacataaa taattttatc
    28441 tttttctaat ttggatatca tatcaatact gttcttttca aatgtcaact tgggaacaat
    28501 gatttgagca tcatagtgag ataattttac tatttcatca tattttaaat aagcagatag
    28561 gagtataagt ttttctttaa aaaactttaa atttccctct tcaaatgagt ataaatttat
    28621 ctttagatta tcacctaaga attgccttat ttgattttca taaatttctc cagcgtcttt
    28681 ttttaaactt acaatagcta attccatttg tttcatttta acctctcata caaatttgac
    28741 ttattttaga ttacataata attaaattaa ataatattat ttaattatta ttataaattt
    28801 attatagact acacattgaa gtaaaagaag tagctaaaat tattcctttt atttttgttt
    28861 tttgtatcta tttggtataa tagtattaga gcgtataata atttaggcaa aattttttaa
    28921 ggaagactag aggagaatat aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt
    28981 caccaaataa taaattagct ataaaatatg atgacaatat gaatatatat ccatgtcgtg
    29041 cagaatgtaa ggaaaaaatt gaagaatata tagcaaaaaa acctacatat aagtttataa
    29101 atatattaga ggttctttta ggtgtgggcg gaatattctg ctttttgagt aaatggacta
    29161 ttgcagccca agttgtactt ggaattgatg cactggttat atttttattc ccattggcag
    29221 gatttaagat gaatggaaag ttaagtatgg aacaaactaa gaatcaaagt aggtctattg
    29281 caatatcaat tttggcattt atagtaataa taactgtatt atattttaaa caagttggta
    29341 agtagagcat aattgtataa gacatcatta gagtatgagg tgagtttatg gaagaaagaa
    29401 aattagcacc aaacaatgag agggaagctc tcataaaaag attaaaaaga atagaaggtc
    29461 aagttaaggg tatacaaaag atggtggaac aagaacgtta ttgtgttgat atattggttc
    29521 aaatatctgc tataaggtct gctataaata gagtaggcac aattatctta gagaatcata
    29581 taaaaggatg tgtagcagaa agcattaaac atgatgatgc tgataaaact gaagagatga
    29641 taaaagagct tatggatact ataaataaat ttacaaaata aggaggcggt tagttatgaa
    29701 tcaacaagga tttattatgg aaattgttga tgatagaact gcaaagttaa aaatgcaaag
    29761 acattcagct tgtgctgctt gtggaaaatg tgctactaca aattcagcag agtcaaaaga
    29821 aatattagta gaagtagaca acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag
    29881 tatggataat atgaatgtat taaaagcagc tgtaatggct tatataatac ctcttatagc
    29941 acttcttgta ggaacaatag ggacttatta tatattgggg gcaattggaa tgacaactgg
    30001 agttgaagcc ataagtggat tagttggaat ttgtcttaca ataatctgtt atttatactt
    30061 aaagaagaat gacaaaaaat ttagagacag tagagaattt ataccagtaa taacaagaat
    30121 attaattgat ctttaagata attataatta aaaaatagca atgtgctttt taaataaata
    30181 ctcaatacgt atttgtagag tttaagtaca ttgctatttt tatattttta ttttgatttt
    30241 ctgtaaggaa taaagttttt tctgattttc aaaagattca atgttcttac aaaagaagtt
    30301 acaactaata caccaagtgc tattgcagca cttagaaata gcgcatctgt accttttgac
    30361 atagccaaag aataattttt ctgaatcaaa gacaacattg tattatatag cccaattcct
    30421 ggtattagag gaagaatacc tggaattacg aacacaatag ctggttgctt tagttttctt
    30481 gctaaagtct cactgcacgc tgagaccaaa gcagcagcga taaaacctgc aaaaactgca
    30541 tttgtagtaa aattaaataa ataaacatat acaatccaac caattccgcc agttactcca
    30601 gctggaagaa gtagatatag tggagcatta aagaaaacag caaatccagc agttgcaaca
    30661 cctgaaaata aaaaatgcaa aatcatagac atatcagtca ttagaaagga cctcctattc
    30721 tataccatgt atcaaggaca aaaccaactc cagaagcaat tgcaataata atcatagcag
    30781 catcaaaagc acgagaaatt cctgatatta aattccctga tattaaatct ctaatagctt
    30841 ttataaaagc aactcctggg agtagtggca ttatagcgcc gactataagc attttagggt
    30901 catctaaaat gcctatttca actagtagaa ctcctgaaag tgcaattaaa aaagaagata
    30961 ctaaacagga aaatgcagat attgcactta tcttcatcgt tttatcataa gtaattgcag
    31021 caaagattgc tattataaac gtaagtataa aatttaatac ataattccct ccaagcaaac
    31081 aagcaaaaca agcagatgca attcctgtac aagagtaaaa taaccataaa ggataaggtt
    31141 ttacatcttg gatttcataa agacgtgcaa tggcattatc aatatcatac tctttgtcac
    31201 taacaaattc tcgagaaaaa ctgtttaata aagaaatctt atttaagttt atacctcttg
    31261 attgaatcgt tttcataaac gaaaagccat caaatttctc atctgaaata attattactg
    31321 taggagttgt aaaaatattt acatgataaa accctcttga cctgcatatt ctaaggacac
    31381 tgtcttcaac cctagatgtt tcagctccat tttttatcat cagttcccct acaaatagag
    31441 ctagttttag aatatttttc ttatattcaa gttcctcatt ttgattcata aatacacctc
    31501 caaattaaaa aacgtaatat tgaaattata tcaaattttt aagtaatatt ccttaatatc
    31561 tatataaata tattatcttt tttttgtttc aaaatagtca catttaaatt atatccaatt
    31621 aaaagaatta gacaatatta tttattatat tttaacttaa aaataaagaa agtaactatg
    31681 ttaaatatgg aaaaatggca aaacacttga ctattaattg gtaaaggact ataataataa
    31741 attatcatat tgataaaaac gaaaattatg aaatgtctgt aaaaataaaa tttaaataat
    31801 aactaggagg agtaatatgt ttgagaactt gaaaaaagcg gaccctgaga tttatgaaag
    31861 tatgaaaaga gagttaaaaa gacaacaaag aaatattgaa ttaatagcat ctgaaaacat
    31921 agtatcagtt ccagtaatgg aaactatggg aagtcatctt acaaataagt atgcagaagg
    31981 ttactcaggc aaaagatatt atggtggatg tgaatacata gatgaagttg aaactcttgc
    32041 aatagataga atcaaaaaaa tatttggagc agaacatgct aatgttcaac ctcattctgg
    32101 agcaaatgca aatattggtg tatattttgc tatgttaaaa ccaggtgatg ttgttatggg
    32161 aatgaattta tctcaaggtg gacatttaac tcatggtgct ccagttaata tttctggtca
    32221 atattataaa ttttatgaat atggtataga taaagacagt ggacttatag attttgatga
    32281 agttagaaag atagcacatg aagtaaaacc taaaatgata gttgcaggag caagtgcata
    32341 tcctagagaa atagatttta aaaagtttag agagatagct gatgaggttg gagcattgct
    32401 tatggtagat atggctcata tagcaggtct tgtggctgct ggtcttcacc aaaatccatg
    32461 tgaagtggct gattttgtta caacaacaac tcacaaaact ttaagaggac ctcgtggggg
    32521 tgttattctt tgtaaagaaa aatatgctaa agacatagac aaagcaatat tccctggtat
    32581 tcaaggtgga cctcttgaac atataattgc ttctaaggca gtttgcttta aagaagcttt
    32641 aagtgatgag tttaaagaat atcaggtaca agttgctaaa aatgcaaaag ccttagctga
    32701 agaacttatt aaaagagatt ttaaattgat ttctggaggt acagataatc atttaatact
    32761 attagatttg actaataaaa atataactgg taaagcagca gaaaagagat tggatgatgc
    32821 ttatataaca gctaataaaa atacaattcc atttgaccca aatggtgctc ttgttacaag
    32881 tggaataaga cttggtactc cagctgtaac tactagaggt atgaaagaag aagatatggc
    32941 aataatcgct gaggctatag acctatgttt aacttatgat gaagaatcta aggctagaac
    33001 attggttgtt ggtttaactg aaaaatatcc tttatatgaa gaatataata ttatggatta
    33061 attataaaga acaactaaat aaaatacatt taaataatta caatgattgt gtatgtattt
    33121 gaatatattt agatataaaa aaggtgtctc aaaatgaact ttttagttta tgaggcactt
    33181 ttttgtatgg aatcgaacat atttgtatta tttaaataat aaaaaatatg gttatctcta
    33241 tttttagatg gtctctttgt attttttaat taaaacttta agatttctta agaattatac
    33301 taggtttttc taaagattta aagtctatac ttataagtat agtaatcaca tgaaaagaaa
    33361 agctttaacg aggagggaat cttatgaagg tattaattct tacaggaaaa tttggtatgg
    33421 gtcattactc agcatcaaat tctttaagtg aagatataaa agcaaaattt gataattcag
    33481 aaataataat taaagatata tttgagtaca ttatgcctaa ctattcagat aaaatgtaca
    33541 agactttttc tattcttgta aatcggggaa gtagtcttta taatttattt tataaatgtg
    33601 ctgaaaatgg aaagaaagat ataaaattta ctttttcaga ttatttttta aataaattag
    33661 atacattact tcatgaagta caaccaacag tagttatttc aacttttcca ttttgttctc
    33721 aattagtatc aagatataag gaaaaatata actcaaatct tcctttaata acatgtataa
    33781 ctgatataag tagtcactca gaatggatta gtaaaaatac agactgttat ttagtagcta
    33841 gtaaatctac taaggaagaa cttgttttta aaggaataga tgaatctaag attaaagtta
    33901 atggtattcc agtaaaaaaa gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa
    33961 aaaatattct tataatgggt ggaggattag gattactgcc taaatcagaa caattttaca
    34021 aagaattaaa tagtttagaa ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa
    34081 tgtactataa actatatggt agatatgaaa atatagaggt agtaggttac accaatgaag
    34141 tatataaata tatgaaagat tctgacctta ttatttctaa accaggtgga ataacactat
    34201 ttgaaacaat ttattcagaa cttccaatat tggcatttaa tccattttta caacaagaga
    34261 tagataatgc tagttttata ttaaataatg aaattgggag aatacttggt aaaaataaaa
    34321 aatactatgt agatgagata aaagatttaa tttatgatga tgctactctt aaagaaatga
    34381 gcagtaacat gaaagaatta aaaaaacaat ttgataataa taccttagag aatattttat
    34441 tttcacttga tgaacaagga gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt
    34501 tgcattgatt tttttagtcc attcaattat acctacatac tataataaac tattaaataa
    34561 agaggtatta aaaaatatgg ctggagaaaa tgaaattgca cttacatttg atgatggtcc
    34621 agataagaga tatacagaaa aattactaga tgtgttaaaa gaaaatgata tacaagctat
    34681 gttttttgta gtagcaaaga atgctgaaaa agagcctgaa attataaaaa gaatgttgag
    34741 agaaaatcat atagttggtt tacattcatt ggaacacaga aatgcatggc tttatagtta
    34801 tagttatgtt aaaaaagatt ttattgaaag tacaaatata atgaaaaatt taggtgtaga
    34861 tgtaaactac tatcgtccac catggggtca tactaatata ttttcaaatt catttgttaa
    34921 aaaatataat ctaaaaatga ctttgtggga tgtaatggca gaagattggg agaaagatag
    34981 tacagtagac attattataa acaagttaat gagtagaaca aaagaaaatt ctataatttg
    35041 tttacatgat gcaggtgaaa attcaggtgg tgcagtaggt gcacctgagc gtactattga
    35101 agctttaaag atagctattc caaaacttaa agccagtggt ttaaagttcg taacaccaga
    35161 aaggatgtag ttatgataag taaaactgaa aagaaaaata aatgcctagg tagtgtagct
    35221 tttttagttt tactaatggg cataacagga tattttgttt ttagagggca gtcagttgaa
    35281 tcattgataa aatcattaaa aggtgcaagc cctatgttta tactaattgg atttgcaatg
    35341 atgtttatat atgttgcctg tgaaggtata aatatatact taggtatgaa agctttaaac
    35401 caaaagacta ctcttttaaa atgtatggga tatgctttta ttggatttta ttttagttct
    35461 attacacctt cagcatcagg tggtcaacca gcacaagttt attatatgaa aaaagacgat
    35521 ataaatatat catattcttc acttatacta ttagtcattg ttgttattca ccaagtagtt
    35581 atactggcat atagtggaat aatgttcatt atggaacgtg agtttatatt aaataatgta
    35641 agtggtatga acattttact aatatatggt gtaataacaa atgttgctct tgtcattgga
    35701 gtaattgcaa ttatattttc aaaaaaactt gtaaacaatt ttataatatc aataacaaat
    35761 ttattaggta agttaagaat aataaaagat gttgaaagtt caagaaaagt tataatatct
    35821 caaattgaag agtatgttaa aggtgctcaa tatataaagc agaatccaaa acttgtagtc
    35881 caaattttag tcataactat tgtacaaata acagctatgt ttttagttcc tttctttgta
    35941 tacaaagctt ttcatctatc aacatatact gtatttgaga tattagcgat acaatccttg
    36001 cttaatattg ctgtttcttc attaccattg ccaggggctg ttggagcttc tgaaaatagt
    36061 tttatgacat tatttaagat atttttccct ggtcacttat tagttccggc aatgttactt
    36121 agtaggggga taagttttta tgcatttgta gctataagtg gattgatatg tatagtagta
    36181 catgtaaaat cttctgaaaa agtaaaagca gtgaaaaaag ttgcttatgt aagataatat
    36241 tatatagatt atttttatta atatgtcaat ttgtgtgtat attttaatta agctttaaac
    36301 atgtttacag gagtagaaac tattaaaacg tgtgacattg ctaagctttt gctaataaag
    36361 tataatattt tatatagttt gaaatattta agaaatatag caaaaaaagg agcttggtga
    36421 tgaataaaga gattccaaaa aaaattttag tagctgatga taatcctgag attagggaaa
    36481 tcgtagaaat acttttaact ggtgaaggtt atgaagttat tatggcaacg aatggagaag
    36541 aagctgtaaa cttagtagat agtacgattg atttgattat tttggatgtt gtaatgcctg
    36601 taaaaacagg atttgttgca tgtaatgaaa ttagagaaaa aacgacagca ccaatattat
    36661 ttttaactgc aaaaactcaa gactctgata aagttattgg attttcagca ggaggagatg
    36721 actatttatc aaagcctttt tcatattctg aactaatttc aagggttaag tctctactta
    36781 gacgttatta tatataccag ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa
    36841 aagagttatg tgtcaattta gatactcaag atgttacact aaatggagaa cctgtgtttt
    36901 tgacaactat agaatatagt atattaattc ttatgttgaa aaatagaaaa aaagtttttt
    36961 cgtctgaaaa tttatatgaa actatttggg atgagcagta tttttataca gcaaataata
    37021 caataatggt tcatattaga aatttaagaa aaaagttaga aaaagaccaa aaaaatccac
    37081 aatatataaa aaccgcatgg ggaaaggggt attatattga ttaacaaaat taagccatct
    37141 aagttgggag ttatactatt gatatatgta gtaatctcag ctgcagtggc tttcatttta
    37201 aatataccat tttactctgc tgttgaatct atagtaaaag atgtatcaga agggaaagta
    37261 tattttaaaa gagaagtaaa tcaacttatg gataattttc aaacttatgt gaataacaat
    37321 ggtataagag cagatgatgg agatgatata gcaaaatgga atagtgataa ttggtttgtt
    37381 tctatgaaga tatataaagg aaataaggtt ttttatgata cattatacta tcctaatgaa
    37441 gtacctgata agagtgagga aaatgagttt tatagcagtc ccatggctag aaactatgag
    37501 atacaatttt ctgatattaa agctaatata gttgtagtag ctttatttag atttagattt
    37561 caaaatttta tagaagcaat ttctattaca tttatggcac ttacatttat tataacattt
    37621 ttactgttgt ttagtagaaa gataaagtac ctaaggaaaa tagaaatggg tataaagata
    37681 gtagagagtg gcagtttaaa gtatagaatc ccagtaaaag gtaatgatga attatcatca
    37741 cttgcatcta gtattaatga gatgagtcat acattggaga atcaacttat aaaagaaagg
    37801 gagatgaaga ataaaaataa agatatagta acttcgatat cacatgatgt tagaactcca
    37861 ttaacgtcag taatttgtta tttagatttg attaaagacc ataaatacaa tagtcctgag
    37921 caattggaac aatacataaa aaatgtaagg tctaaagcat atcaaataaa agatttaaca
    37981 gatgaccttt ttacgcattt tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa
    38041 ataaatgcta atgaatttat ttttcaatta cttttagata gttcatatac attggaggag
    38101 aaaggatttc atataacaat agaaaatgaa ctttataacc aatatcatat ttatgtagat
    38161 gttacacaat ttagaaggat atttgataat ttatgttcta atataataaa atatgctagg
    38221 aaagataagc ctatagtatt tttaattaaa gtagaggatt ctaaattact aattatacaa
    38281 aaaaatagag taagtaattt ttctaagaat gtagaaagtt ttggaattgg tataaagaat
    38341 tgtgagaaaa tagttgcata tcataatgga agcataaatg taaattcaga tgataaagaa
    38401 tttgctattg aaatatctat tccagcaaaa gaatcataaa aatttaaatt atatattgtg
    38461 taattgctta tgtaaaattc tatgaagcag gaagcatgtg actttagtta tgggatgttc
    38521 aatattgtgt acctctaggc aagtaacaag tttacttaca agaccatata atattattat
    38581 cttaaaaaaa ggggacataa taatatggtt ttgacaggag cacattatat ttacattcta
    38641 tttatggtaa ttatattaat tactatgatt atgaaaaagg atacaatagt accatgtata
    38701 ttaggtgttt tttttatggg gttgttcttt gaaaaaaaca tttttggtgc tataacagca
    38761 gtgtttaact cttttattat atctttaaat gagttgggtc caattatctt gattatagct
    38821 attatggttg cattatctaa agccctagaa gccaataatg ctatacagta tatggtgaga
    38881 ccattttcga gagttataaa aaattctaat acagcatttt ttgtaactgg atttgttatg
    38941 ttagttcttt cttggttttt ctggccaact ccagctgttg ccttagttgg agctgtattt
    39001 ttacctgttg ctatgagagc tgggctacca gcaattggtg ttgcagtagc attaaattta
    39061 tttggtcatg gtcttgcctt atctacagac tttgttattc aaggagctcc aagtataaca
    39121 gctggtgcag caggtgttgc agtatcatat gttataaatg atggtatgat tttgttttgg
    39181 gtgatgggaa tcgtaaccat atctatggca ttttatacat taaaaagaga tattaacaaa
    39241 ggaatgttta gagaagaact aaaaggtttt gaaagtgaag aagtagaaga gtttaatgga
    39301 aaatctaaaa tagcaactat tttagtagct ttgggatttc ttgctgatat aattgctatg
    39361 tatgtatttg acctaaaagg aggagatgca tcagctcttt tgggtggaac agcagtattt
    39421 ttaataataa ttataaatgt tataaatttt ggcaaagata gtcttgaaaa tgtatgtgag
    39481 aatataattg atggttttgt gtttgggata aaaatatttg gagcaattat accaatagca
    39541 gcatttttct atatgggtga agttgcacca ttgacaggtg tatttggaaa agtattagca
    39601 ccaggttcac aaggtttatt atcagatata ggcatagctc tttctcaaac aataccactt
    39661 aataaatttg ccgtatcagg tatagaaact gtagtgggag caataactgg gcttgatggt
    39721 tctggatttt ctgggatttc attagttggt tcacttgcct ctgtatttgg aactgctata
    39781 aatgcaagtg ttggtgcact agctgcatta ggacaaatca gtggtatatg ggtaggtgga
    39841 ggctgtctag ttccttgggg tcttatttca gcatcggcaa tttgtggagt tagtccaata
    39901 gaattggcaa agcgaaactt tgtcccagtt ataaccggat tggttgtaac aaccatagta
    39961 gcaatgttta ttatttaata aatcgaataa atctttaatt gtataaatta aaaatagatg
    40021 tgtagctata atttattata attgttttac taattctaag ccatacatct atttttattg
    40081 atatttttat tagtattctt taataaaata atatttttca ttatactata cataacatct
    40141 aaatataaca tctaaaatac tgttatctat atttaatatc ttgccaaatt ttttgatgtt
    40201 ttattgccct gtcccaccaa catatataag ctatcacaag tttttaccta tactatcacc
    40261 aaacaaaaat tctaatttta atttattatt ttataaccta tattatatta actatattag
    40321 tttgtagact ttagatgtaa ttataaatat taatactcca agagttcaaa ttcatatcct
    40381 agttttcttg catttgatat aaaatcattt aaaatttctg tactagtttt ggaaactgca
    40441 tgtagtaaca agatagaacc atcatgtaga ttatccatta ttttttgcgt tgcttcttca
    40501 tgtgatggtt gtttatcagt atcccagtct ctgtatgcaa aactccaaaa tacagtcttg
    40561 tatcctaaat ttttagtcat tgccaagctt ttttctgagt attttcccat aggaggtcta
    40621 aaaaatttaa ccatatcttt cccagttaca tctttatata acttttcaac atcatataat
    40681 tcgtcattaa agttttttag attgcttgtt tttgttggca tagatggatg ggttttacta
    40741 tgatttccaa ctatatgtcc ctcagaaacc attcttttta ctaaatcttt attttctttt
    40801 atatatggag ctgtaacaaa aaaaactgct ttaacctgat tttgttttag agtgtcgagt
    40861 atttttgtag tgtatccatt ttcataacct tcatcaaatg taagatatag tgttttttga
    40921 ccatcttttg taggtccatt gtatagggca tcatagtcag aaaatttaaa ttcaatgtcc
    40981 gtatttactt ctggtgtttt atgcttattg tttggtatat aaaaccaatc aagagtagtg
    41041 ttatctaaag aagatacatc taatttatta ttatcattat tagtagtggc ttttgatggt
    41101 ttatctgttc cgtctgaaac tattacattg gaagtatctt taccactatc tattttttcc
    41161 ttatcttctt gtaactgtgt ttccttattt tgattttcgt tttgattgtt ttgtgaattt
    41221 gaacatccaa caataaaaaa aatagaaata gaaagtagtg ttaaataatg caatactttt
    41281 ctaaacattt tgaagctcct ttctaaatta ataataattg catataataa tgattatatg
    41341 attaattaca attgcatata gtattattat aattggaaat tactattaat aatagttata
    41401 tctttaaaaa taaagtcaca atagtatgac aaagggtgaa taatcaataa atttaaaaaa
    41461 aaatgaaaaa aatagttgac catattatga gaagatgtta ttataataaa tgtgctcaac
    41521 aagaagtaac aaaataacaa gcacaaagaa gttaattgaa t
//
LOCUS       DAENCV010000027        39933 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAENCV010000027 DAENCV010000000
VERSION     DAENCV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.027"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..130
                     /locus_tag="KQD82_001328"
     CDS             <1..130
                     /locus_tag="KQD82_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG4379806.1"
                     /translation="KEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQLSMLS"
     gene            187..324
                     /locus_tag="KQD82_001329"
     CDS             187..324
                     /locus_tag="KQD82_001329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379807.1"
                     /translation="MSNKKKYTLSITEELYNKCKEKANQKGMSVNEYILSVISKNLKN
                     I"
     gene            complement(336..503)
                     /locus_tag="KQD82_001330"
     CDS             complement(336..503)
                     /locus_tag="KQD82_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG4379808.1"
                     /translation="MPSKLPRYTLRIDNNLLEKIKYIAESEGRSANKEIEQLIKKHVE
                     SYEKINGKINV"
     gene            656..802
                     /locus_tag="KQD82_001331"
     CDS             656..802
                     /locus_tag="KQD82_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379809.1"
                     /translation="MPDELNEELHRQSQRKGLSKNAFIINILWKEFEDLQDLKDEQEV
                     DKYE"
     gene            795..1109
                     /locus_tag="KQD82_001332"
                     /pseudo
     CDS             795..1109
                     /locus_tag="KQD82_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1374..1647
                     /locus_tag="KQD82_001333"
                     /pseudo
     CDS             1374..1647
                     /locus_tag="KQD82_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1637..1786)
                     /locus_tag="KQD82_001334"
     CDS             complement(1637..1786)
                     /locus_tag="KQD82_001334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379810.1"
                     /translation="MIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQTK
                     KGPKK"
     gene            1848..2045
                     /locus_tag="KQD82_001335"
     CDS             1848..2045
                     /locus_tag="KQD82_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379811.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            2075..2518
                     /locus_tag="KQD82_001336"
     CDS             2075..2518
                     /locus_tag="KQD82_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379812.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            2519..2665
                     /locus_tag="KQD82_001337"
     CDS             2519..2665
                     /locus_tag="KQD82_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379813.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            2668..2985
                     /locus_tag="KQD82_001338"
     CDS             2668..2985
                     /locus_tag="KQD82_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379814.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEEIERDRPID
                     IQI"
     gene            3063..3572
                     /locus_tag="KQD82_001339"
     CDS             3063..3572
                     /locus_tag="KQD82_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG4379815.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            3582..4190
                     /locus_tag="KQD82_001340"
     CDS             3582..4190
                     /locus_tag="KQD82_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG4379816.1"
                     /translation="METNNVYIKLVNIQNTLKAPKNQYNNFRKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKISTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            4200..5081
                     /locus_tag="KQD82_001341"
     CDS             4200..5081
                     /locus_tag="KQD82_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG4379817.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDAKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDSTANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            5142..5315
                     /locus_tag="KQD82_001342"
     CDS             5142..5315
                     /locus_tag="KQD82_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG4379818.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            5331..5738
                     /locus_tag="KQD82_001343"
     CDS             5331..5738
                     /locus_tag="KQD82_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG4379819.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            5808..5969
                     /locus_tag="KQD82_001344"
     CDS             5808..5969
                     /locus_tag="KQD82_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379820.1"
                     /translation="MSRTAICSVCGEFLVESYDDQFTNTKVMDLHGREFVIVICKSCL
                     EELLRKCED"
     gene            5974..6195
                     /locus_tag="KQD82_001345"
     CDS             5974..6195
                     /locus_tag="KQD82_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379821.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQHKKYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFWFNFGVVSEWCVEVK"
     gene            6213..6593
                     /locus_tag="KQD82_001346"
     CDS             6213..6593
                     /locus_tag="KQD82_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379822.1"
                     /translation="MAKIWLDAGTFLEKTIDIEDMFELNLRKVRKANESKKIKLKLNE
                     SKFKKIRKKPTNDTKSKPIKVFNIETGEVRIFKSAKAASKYLKISGDYASCLARENRV
                     TREGWKAEYIQEVSDGISKCGASN"
     gene            6562..6711
                     /locus_tag="KQD82_001347"
     CDS             6562..6711
                     /locus_tag="KQD82_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379823.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNESN"
     gene            6698..7147
                     /locus_tag="KQD82_001348"
     CDS             6698..7147
                     /locus_tag="KQD82_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379824.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKENCEWQKVS
                     NVGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLS"
     gene            7250..7426
                     /locus_tag="KQD82_001349"
     CDS             7250..7426
                     /locus_tag="KQD82_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379825.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            7540..8229
                     /locus_tag="KQD82_001350"
     CDS             7540..8229
                     /locus_tag="KQD82_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379826.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            8247..8456
                     /locus_tag="KQD82_001351"
     CDS             8247..8456
                     /locus_tag="KQD82_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379827.1"
                     /translation="MSKYVLRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFKNL"
     gene            8468..9463
                     /locus_tag="KQD82_001352"
     CDS             8468..9463
                     /locus_tag="KQD82_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG4379828.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFEDDKFNIQMSKNEINIQETKIVKIAVIVG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKMFKAFVDSYIAHLY
                     SDMKQGEDVRGMLLYMLREKQKLDINEFADKAIKDDLKDSFKDHIEEKGIESFNIDKK
                     WVEKNLKNRHIKTDTGFEIKGKMDDFEDFMKYGIRHNGNGTVDIVIKNVNFYNEK"
     gene            9472..10197
                     /locus_tag="KQD82_001353"
     CDS             9472..10197
                     /locus_tag="KQD82_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBG4379829.1"
                     /translation="MKYVASFSGGKDSAAMLLLILEKKLPLDEIVFIDTGLEFEEIYD
                     IIDGFEKRINFKITRIKAEKTFEEYFYTVNKQGKHKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNSIGEHKRYIGIAFDEPNRYKRLEKNCIAPLYEAKMTEKDCLKYLEEKGFYYE
                     IHHRFKRTGCYLCPKQSLDSLRTLRKYYPDLWGSMLKLDKDSPVPFRANGVTVHDLEK
                     RFSNEDIENERQISFFQTEKVSI"
     gene            10194..10355
                     /locus_tag="KQD82_001354"
     CDS             10194..10355
                     /locus_tag="KQD82_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379830.1"
                     /translation="MTNKEMCKSNNLDEREVYKKFGKEICGSCINDKVDCESKDCDTT
                     YKNWLEKEI"
     gene            10358..10666
                     /locus_tag="KQD82_001355"
     CDS             10358..10666
                     /locus_tag="KQD82_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379831.1"
                     /translation="MKINLNDIPEGYKVAVIPILEAIKRAKEDEKELREYLKEIEECK
                     KINISIVRKNGTKIPLTFTNKELNIRLKEEIKLAIKCIKEEATEIKELLIKMESEGNK
                     "
     gene            10759..11046
                     /locus_tag="KQD82_001356"
     CDS             10759..11046
                     /locus_tag="KQD82_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379832.1"
                     /translation="MITTQMYKINNEIYLNNICLEENKEITIWVEKNNTHELIWLKIA
                     NVNGKLAIFVQDIENAVIKEWQGRKEYERVLGQIEDKQVEKGEILYEEYWK"
     gene            11027..11233
                     /locus_tag="KQD82_001357"
     CDS             11027..11233
                     /locus_tag="KQD82_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379833.1"
                     /translation="MKNTGNKVEINNIRELNDALNKYDIPFGILSDVDRRICDWMATG
                     GNEDDAYIKQQYRYVENFINRFCD"
     gene            11264..11449
                     /locus_tag="KQD82_001358"
     CDS             11264..11449
                     /locus_tag="KQD82_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379834.1"
                     /translation="MVITEFKVTWQTTREWEKGNVYSSIFTDLEEARAFKKDEENIKE
                     NYNVKLLSRAIVEIEID"
     gene            11587..11994
                     /locus_tag="KQD82_001359"
     CDS             11587..11994
                     /locus_tag="KQD82_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG4379835.1"
                     /translation="MKVFLVIDGEPIGKERPRFILATKRTYTPNKTRDYEELIRWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            12096..12944
                     /locus_tag="KQD82_001360"
     CDS             12096..12944
                     /locus_tag="KQD82_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG4379836.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKAYK
                     NKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNSYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            13097..13579
                     /locus_tag="KQD82_001361"
     CDS             13097..13579
                     /locus_tag="KQD82_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379837.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGTTYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            13893..14648
                     /locus_tag="KQD82_001362"
     CDS             13893..14648
                     /locus_tag="KQD82_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379838.1"
                     /translation="MSNKIKLEVPNEIGYIIVQCFNKAKEEIREYIGKQNLITKNGVP
                     FQFWDLLNDAIDTAFNSSNFKAYKSKRGRWRMIFIYDKNSKYLYVLMREQRYKELHNN
                     VNKREKMHYVDILTKVFNGDLKAPIEQLSFFSLEFDNEDKIEIYLQKLIDSINKDGAI
                     IEHHVLLLFNQTAEYELTSIRAVMVDTNLNIVSEQNLSSYISIEESTVVEKVDKTNPV
                     ANNPTLGLKLTAKADARKKTHPLKKAKKEEDAK"
     gene            14682..15830
                     /locus_tag="KQD82_001363"
     CDS             14682..15830
                     /locus_tag="KQD82_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG4379839.1"
                     /translation="MEINFNGERLKKARIYRGMTVAELAERIDCQRQTVSMYENNKSK
                     PNDNNVVKRIAKELGFPVKFFLETGNNIAIGSTYFRALLTTNKKYRAEQIQKMEFLAE
                     IYFFLQDYIEFPTLNLPDCSGKTPEEAALLLRETWGLGLKPIDNIIYEVEQHGILVTS
                     FSTSTDDIDAFSQMVDISGETFYLIGYSSNKTSASRIHFDIAHELGHICLHEWSEDVE
                     ALEKQEFKDRESEANRFASAFLLPEETFKIDAKRTPLRIPNYTELKRKWKVSIQAMIR
                     RSYSLGIISMDEYHSMIRTLQRRGLRKSEPLDDELLTSLPALLKTAVLMLLNEKVFTP
                     KEFMDELSFSYNFSLEPEEVEYLLSLPKNTLTSAKVIPFPDLQLKKDV"
     gene            16463..17140
                     /locus_tag="KQD82_001364"
     CDS             16463..17140
                     /locus_tag="KQD82_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG4379840.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            17203..17886
                     /locus_tag="KQD82_001365"
     CDS             17203..17886
                     /locus_tag="KQD82_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379841.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKGKEEMVKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLNKDDTDKEITVRV
                     MKASGENGK"
     gene            17876..19219
                     /locus_tag="KQD82_001366"
     CDS             17876..19219
                     /locus_tag="KQD82_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG4379842.1"
                     /translation="MESKFISNDHFYDFIFNWDYKFYFLVGGYGSSKSYHVATKLIIK
                     LLEEKRKALVVREVYDTMRDSCFSLLEEVAESMGLGYILSFKTSPMRVIFPNGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVTKNNTYYHHSVADDNFFLPESYIEQLDEMK
                     EYDYDLYRIARKGRFGVNGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQELIKADSAEPKTIKYF
                     RQQGFNIFGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYGLDGYEVSDIKELKYSNDIYNKGLGLKKNNQYNKKGGTVF"
     gene            19219..20742
                     /locus_tag="KQD82_001367"
     CDS             19219..20742
                     /locus_tag="KQD82_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG4379843.1"
                     /translation="MNIKNILLNLDEKELRDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIVDNKVKPLINKQARFMFGKEPTIILKAYEKEHKEACEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSINDFKYQVDSN
                     DITKLKSVVFVRFDSSTIKEVTAKQIWYRYTYYMKKSDVSNQESCFIKIEKFKGDNLS
                     KPIKITENDTKLSKIPCWVIVNEQSITNIRGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKSKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDNIDEELNSKSNNSDE"
     gene            20755..22344
                     /locus_tag="KQD82_001368"
     CDS             20755..22344
                     /locus_tag="KQD82_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379844.1"
                     /translation="MKDNTYTKKVLEVRKKLLLLDKKVQLEILNVYKDASKTILSDIA
                     KNKELNLSTKYLKKLNKSIEKYINELNQRLVPVTEKSITEASNIAKDLQMYYYQSIVP
                     NKSINLACDAMCINTTTNVVEKIVAGNFYKDKRSLDGRIWGYNNKNRKDIDRLIKANI
                     ARGANAKTLAKSLDNYVNPVKKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCVGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRFGLGEGVFPPEKYPIPHP
                     NCLCYPTQIIVPINEASKFMNDWLNGMDNSNLDSWYDEINTETTNLYIPKIKYSSNDV
                     KSKYGKTLNKKINSFESKIENTSSKYKKYTTGVKYEVTNELKTAFAYSIKDDIIKINP
                     LSPIFKEYNFELSMMHELGHRVDLKELEIYKNIEFKDAILKSSNYVLNNLKEIQIEYD
                     YLVNINDSPFLSDILGALSFNKIEGLLATHNITYWQKQGNIEKEIFANLFALDYESDK
                     HILSFIKKNIPDIYNVFNKVI"
     gene            22357..22527
                     /locus_tag="KQD82_001369"
     CDS             22357..22527
                     /locus_tag="KQD82_001369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379845.1"
                     /translation="MGEQMPIMKRLRNDKELLELRRLYKEKYNENAPGFNYDEYSSYD
                     EYKEKLKELIQK"
     gene            22596..23249
                     /locus_tag="KQD82_001370"
     CDS             22596..23249
                     /locus_tag="KQD82_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG4379846.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRYVEKTE
                     LENANKEIKEYKKQMGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIRESAEKEIE
                     VLKFNTAFERVIESYNPRNSKALAALINKDNISFVNGKFIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDEIGGTGGLEGGTTSLLDNDSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            23261..23617
                     /locus_tag="KQD82_001371"
     CDS             23261..23617
                     /locus_tag="KQD82_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379847.1"
                     /translation="MSIEKSEIYMGENKTTLKFAGNLFQNVNIKVKKTDVAIVDGKRI
                     LKAGTLVSKDGKLVDGTTVTNDKAFGLVYRNIDFTYSNGNESIPVLIFGFVDEKTLPS
                     VIPEEAKQAMKMIMFL"
     gene            23641..24669
                     /locus_tag="KQD82_001372"
     CDS             23641..24669
                     /locus_tag="KQD82_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG4379848.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGNPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            24686..24886
                     /locus_tag="KQD82_001373"
     CDS             24686..24886
                     /locus_tag="KQD82_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379849.1"
                     /translation="MAKKKENLMQVKALVYLKYDNECYKIDDIFEIRKADQELMEEKG
                     YIEVIGEVEEKNNNELLNKDGE"
     gene            24889..25206
                     /locus_tag="KQD82_001374"
     CDS             24889..25206
                     /locus_tag="KQD82_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379850.1"
                     /translation="MSITDLDKLKLNLQEEEYPYFTDEQLEMLLESNENNVLKASWRG
                     CLLKGATDDEIKIGPIETKSSNSSYWLTLADIYKTDYLEEKSKNETTITGYKTSMTRA
                     DGQ"
     gene            25203..25586
                     /locus_tag="KQD82_001375"
     CDS             25203..25586
                     /locus_tag="KQD82_001375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379851.1"
                     /translation="MRKLRADKIIKTINRGIALNPQTITIEQEVKNIVDGAIDITNEV
                     KKLTVVIYPEKTNDTVINSETIGTAYKNKNFGMVADKEADLRLNTENEITFKCIEGTM
                     KLNYVNPIVVEEQVCGYICGLEKLD"
     gene            25599..26009
                     /locus_tag="KQD82_001376"
     CDS             25599..26009
                     /locus_tag="KQD82_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379852.1"
                     /translation="MSVFTKAINEIDRKKATMPILCMNIAFMLEGEAKNNAKWTDRTG
                     NARQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHVIRPRNAKALFWNGASHPVK
                     QVQHPGTKGTHSLESIVSKNMPKIGKLVEGHWSK"
     gene            26011..26835
                     /locus_tag="KQD82_001377"
     CDS             26011..26835
                     /locus_tag="KQD82_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379853.1"
                     /translation="MRAGIRKALIENIPRIKDCYEPTVPNKKTIKPYIVIVQGEDADN
                     EGNAIGFRRTINIWLYEKRTTFNKLDELTKEVVEALDFKTITDDTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALEDKEDTTNDRWVEALSKHTKDLLEIESYKDNWKKN
                     FIAPCVLWRTTNVKNKRINYHLIEITKTMKCHVVSKNKDEIVKLLEALETSLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLR"
     gene            26848..27051
                     /locus_tag="KQD82_001378"
     CDS             26848..27051
                     /locus_tag="KQD82_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379854.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIENSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            27053..28474
                     /locus_tag="KQD82_001379"
     CDS             27053..28474
                     /locus_tag="KQD82_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG4379855.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGEFGKVVTIKNDLRQLKTLFGDDVNYSAYKLGKLALLGNVKELLLYRLVDGNQKVGT
                     LTLKDTTENTAKDVIKLETKYPTSRNFNITIKSNLVDADKKDFIFFEGTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTTLANVVNQALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KGFNDENIVNIGSSAYYENIKYTPSEVAVYIAAISVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLR"
     gene            28490..28903
                     /locus_tag="KQD82_001380"
     CDS             28490..28903
                     /locus_tag="KQD82_001380"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379856.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            28987..29481
                     /locus_tag="KQD82_001381"
     CDS             28987..29481
                     /locus_tag="KQD82_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379857.1"
                     /translation="MSEIYKRELENEVVEEDNDLDEEIEETNEDRLKMKEDEIIAKLL
                     EDSPVPQRTVFLDRLGIPITLKALTEKEISKIRKECTKIVKVQGRREEKLNDDEFTLA
                     LIEKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDD
                     DIKN"
     gene            29658..33932
                     /locus_tag="KQD82_001382"
     CDS             29658..33932
                     /locus_tag="KQD82_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG4379858.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPIPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSTLGGLTGKIDSFV
                     SGAISKFARLATTTGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYIGSVGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGS
                     LREKLGGLEKVEKGAAIQSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRGFVEDLIKKMPQIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLASTFGTAKLAAFGL
                     TVGIGAIVIAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSSDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLNFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNIGGLKDGVTGMLNSLKATGPQALETLKGLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNSLGLEGTQAIDTLRTIFSQASSALDTTNLKQGLSNTFN
                     SFKEGFNSGGIKNAINSMLGTINQAGPQMQQALGKMDGKMGQVFANVDFSTPIETQAS
                     KVLENLNNLGIEGPKALETVRSIFAQASSQIQGSASQTAQQANQEVANALTQGNPAVQ
                     QAGQQLGTDLTNGVVNGVQAGVPVVQQKSNELATATQNGVTNAVNSATPQIDNSNLTS
                     GIDTAFNQATATVQQGASSMYNGAKQSFTQLAQIGREAGSSLYNGATTSFNMLATNVR
                     VACSSMYNGARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPI
                     TASFNVKTTQTTVKRTVNESSGGILGNILDRFADGGVATRTSICGENGAEMVIPLTNT
                     RRSRAIELYEQTGKMLGLGSTPSQKVGNSNIVNNVRQFPLANVLDTDNKEYKEATPNS
                     VNSSNNTINLGGISINVQNSDNKEEMIQEILSQVESEIREALQDIG"
     gene            34014..34742
                     /locus_tag="KQD82_001383"
     CDS             34014..34742
                     /locus_tag="KQD82_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379859.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLVVFSIEFLMKAIEAKN
                     KGKIVLGCFLALLTLSCARTVYLTRGLDDKNSTQVNQVSNNKDKEDSEKKIEEETKKQ
                     EEVKKKEEIEKNSKEENKGNVETKKQTLTGEELKKKVDSIIPAEYKGNYYTNDVLDTD
                     GSYVLSLQVQNASFDNESSCKAFTKDLINKLKEFRIDSAEVYFVGSSGQTTYQINIDD
                     FLKVQDNINSVDNMEFFSFKDFKN"
     gene            34812..35483
                     /locus_tag="KQD82_001384"
     CDS             34812..35483
                     /locus_tag="KQD82_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG4379860.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPFDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDASYCRYSELKNPIEVVVMLEKWVDQAEPLRLIVT
                     GFGYNGLVTISNFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNNKTTPKIYTVKSSDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            35480..36442
                     /locus_tag="KQD82_001385"
     CDS             35480..36442
                     /locus_tag="KQD82_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG4379861.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIVLIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNISYYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKESKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGTLEYIRRKFYTND"
     gene            36435..36800
                     /locus_tag="KQD82_001386"
     CDS             36435..36800
                     /locus_tag="KQD82_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379862.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVSG
                     YKDEIQDYLVLENLTLKEDYFTFSDEVSSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            36775..37233
                     /locus_tag="KQD82_001387"
     CDS             36775..37233
                     /locus_tag="KQD82_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG4379863.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRHKRSAYSNIYGSEVKELIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVECEFNVLTIDDENIVIDGNIKR"
     gene            37242..38375
                     /locus_tag="KQD82_001388"
     CDS             37242..38375
                     /locus_tag="KQD82_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG4379864.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKAIGTITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGANEKVDIKAECRIAGTIGNVSNNTITVL
                     LGSISGIKSVTNKEDLKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWAGAGTVKVLILDKNRKAATQELINKVQEYIYPNVEEGQNRDGKAPIGAI
                     VTIATPQTLLINVKASFVFSNGFSQETVLNILKNKIDKYLDKIDIGGTVSYNAIYSLA
                     GSMMLADEGIQDFSNLTINDGTTNIILNDQVVGIGEIINEVIA"
     gene            38372..39142
                     /locus_tag="KQD82_001389"
     CDS             38372..39142
                     /locus_tag="KQD82_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG4379865.1"
                     /translation="MITSKKGREMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLDEE
                     IELQLFPQSATWGLEFWENRVGLYTNIDEDIEARRRKVIAKLQMKYIVNPDRLATIIK
                     SYTGADVYIKENIAPYVFKVTANVDDVINYEDFKYITNKTKPSHLHWMPSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWLLNGSKNLSAYILYNEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGIEMLNGNKLLNAELREEVL"
     gene            39143..39523
                     /locus_tag="KQD82_001390"
     CDS             39143..39523
                     /locus_tag="KQD82_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG4379866.1"
                     /translation="MANAVTTDIARQKMCKARAGDITLPTIVSMVFGDGGIGNDGNII
                     APLSSDIALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKTFGNKYKDSDMEMVFQIDDEF"
     gene            39628..>39933
                     /locus_tag="KQD82_001391"
     CDS             39628..>39933
                     /locus_tag="KQD82_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG4379867.1"
                     /translation="MLDNDKYLNNKIDTTKTELNTRIDTENEKQNIKIDQLIAGGSNV
                     AYTQRVAIDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIE
                     "
BASE COUNT        16655 a         4121 c         7660 g        11497 t
ORIGIN      
        1 aaaagaaggt acaagagtac atccagatgg tcatacaagt attactaaaa cacctaagat
       61 tactggtaag ggacaaatat actttattaa taagtttaaa aagaacaatc aattatcaat
      121 gttaagttaa agattaatag taccttgaaa actaaataca gaatattttg aaaaggaagt
      181 gactacatga gtaataaaaa gaaatatact ttgagtatta cagaagaatt atataataag
      241 tgcaaagaaa aagctaatca aaaaggtatg tcagtaaatg agtacatact atcagtgatt
      301 agcaagaatt taaaaaatat ttaattttta ttaacttaaa cattaatttt gccatttatt
      361 ttttcataac tttcaacatg ttttttaatt aattgttcta tttctttgtt ggcagaacga
      421 ccttcacttt cagctatata cttgattttt tcaagtagat tgttatctat tctaagtgtg
      481 tatctcggta attttgatgg cataaatata gcaccttcct taagtctaaa tgatgtcact
      541 attataccac gtaaattctt tcaaaaaaag tattgacgca aaagtgacgc aatgataata
      601 taataaacaa ggaggtggtt caaaagtgac ggcaaagaag agggtaactg ttagaatgcc
      661 agacgaatta aatgaagagc tacatagaca atcccaaaga aaaggattaa gtaaaaatgc
      721 ttttataata aatatcttat ggaaagaatt tgaggattta caggacttaa aagatgaaca
      781 ggaggttgat aagtatgaat aacttacaaa tatttgaaaa gatggagttt ggacaaataa
      841 gaatggcaga gattgataat aagccttact ttgtaggaaa agatattgca aagtcattag
      901 gttataaaaa taccaatgat gcaattttaa gacattgcaa aggggtagtg aaacacgagg
      961 ggtttaaaat taatggtatt aagattgctt tgataactga gggtgatgtt tacagattaa
     1021 tagtaggttc aaatcttcca aacgcagaaa agtttgagag ttgggtattt gatgaagttc
     1081 ttccaactat tcgacaaact ggtcaatatc aagcacaaca aaatgtaata acagaactta
     1141 caggaactat aggagattta aaaggaacaa taaaagaata taaaaagttg tgtaagataa
     1201 catgctctaa gaaacaacag tattctaaat acataaagaa tagactggat atagacaaag
     1261 ctaataaaga atacaaccaa gtgaaagcaa ggctattcct aattcttgga gttgagaagt
     1321 gggaagacat agacttcgat acatctaata atttaataca aattatagat gattgtataa
     1381 aggttataaa gtcagaaaga ccatacaagc agttgagtta ttttgaaaac taaatacaga
     1441 atattcaaaa gaggtggtta aatgaacatt aaaaaattag tactagcaac aggtgaagaa
     1501 tttgagaaca tagagctcaa agagtttgaa aataaaggag aatgcttacc atcaaatatg
     1561 gtaaaggtta ttgatggtaa gcaggaactt ctaataaata aagagttcat attatcatta
     1621 gaagtcaaaa gaaagactac tttttaggtc ctttcttagt ttgtgacaga gcactaccag
     1681 caaccgattt ggaagcagca ctatatcttc cgtctttaag aatcttacta gccttagaag
     1741 caacagtttt acttgtttgt ttagtatttt tagctatagg tatcaccacc tttaaatgta
     1801 tttataggat atatcctaca aatatagtat atcaaaggag gaaaataatg gcaattaatg
     1861 acaacataaa taaaatttta aaagatagag atttaaaagc atggaaatta gcaaaagaaa
     1921 taggtgtaga ttcagggaat ttatatgcaa ttttaagagg agaaaataaa aatccaacta
     1981 tagatacatt aataaaaata gccgactatt tagacattac attagatgaa ctagttggaa
     2041 gataaaaatt aaatacagaa tattttgaaa aggagtgagt aaattgggca atatatctaa
     2101 tttcaattta gataaacaag aagataaaag ttttaatgac ctagataata tatcaatttg
     2161 tttttcagaa ggtatccgta aagttgtaga aatgaattta aacaactata aaaataaaat
     2221 ctcaaagtac ttaaatgaaa cttcgaaaat agaattatta gaaccaaaag aactaacaat
     2281 tgttataagt aaaggttatc ccgattatct tatgtctgtt gaagaagcaa gtaaaagatt
     2341 gaaaatagat aaagtatttg gatatgagtt aataaaaaat gggcttttga agtcagttga
     2401 tataggggca actaaagttt ctagttatga attagatgat tttataacta gaaatcaagg
     2461 aaaaaacatc aaagaaatgc ttagagaaat gaaagaaatt agagaggggg tgatttagtt
     2521 gaatgtaaga gtactaatag cttatgtaca gttttgcaat gataagcaaa taaaagcaag
     2581 ttttgaaggt cttagaaaat acaacagaga tatagagtta gttaacttgt atttaccaag
     2641 taaattaaaa attaaggggg attaatcatg aaaagtttaa ctatagtaag aaatgcagta
     2701 gagcaacaac taaatagagc taatttagaa ataaataaaa atgaggaact ttatacaaaa
     2761 cttagaaaaa aagaaaaaag agatgtatta gatgaaatag agttaagtaa tgctttaaga
     2821 gaaaaaagtg taaatgaaag attaaaaata tttgctgagt cattactaaa aattatagat
     2881 acacaaattg aaataaaaga atatgaagaa agcgaggatt acaagatatt tgaattaatt
     2941 tcagaagaaa ttgaaagaga tagacctata gatattcaga tatagaaaag agccattagg
     3001 agtggctcga ttccaaaata atataaaata taagctaatt atagcacaaa cggagggaaa
     3061 ttatgagtac tttatatgaa ttaactacag atttattaga aatagaagaa ggtttaacag
     3121 aaacaacagg aaatgaagct gaaaaactag aggaaataaa agaaataata aaacaagaga
     3181 tacaaaataa aaacactaga atagtgtcag taatattaaa cattgacagt gatataaact
     3241 ctatagattc agagattaag aggttacaag agttaaagaa ggttaagaag aatactcttg
     3301 atagattaaa aagcaatata aaagactgta tggaattact tgggactaaa aaagtagaaa
     3361 cagttttagg aaatataagt ataagaaagt cagcaggtag cttagtcata gaagatgaag
     3421 aaaagatacc tgctatatat aaaacagtag agcaagttgt aaaagtagat aagaacacta
     3481 ttaaagattt tattaaaaaa ggtcatgagg ttgaaggttg caggattgaa tatggaacta
     3541 cactaacaat tccaaaagct aaaaaagagt aggtgaggac catggaaact aataatgttt
     3601 atataaaact tgtaaatata cagaatactt taaaagctcc taaaaaccaa tacaataatt
     3661 tcagaaaata caattacagg agttgtgagg atattttaga aggtttaaaa cctattctaa
     3721 aagaagaaaa agcattggtt atattggatg ataatattgt tcagatagga aatagatttt
     3781 atgtagaagc tacagcaact ttaatagatg cagaaacagg agaaaaaata tctacaaagg
     3841 cattagctag agaagatgaa actaaaaaag gtatggattt agcacaagta actggaagtg
     3901 tatcaagtta tgcaagaaag tatgctttaa atggattatt ttgtattgat gatacaaaag
     3961 acagtgatgc aacaaataaa catggaaatg agcagaaaaa aaaagaagtt aatgagagtg
     4021 aattaaatat actatattcg ctaggagaat ctatagaaaa agataaaaat agagttgata
     4081 gtgaagtata taagaagttt ggaaagttag cagtagattt gactaagcag gagtatgaga
     4141 aagttttaaa tggatataag agcattttag agaagcaaaa acaagagtag gtgatagtat
     4201 tggggattat aagagtaagc aaagacaaag ataatccata tgtagtttta aataaaactt
     4261 gtttggaaga tgcaaaatta agctggcaag caaaaggctt acattcatat ctgattagta
     4321 agccagacca ttggaaaatc tatgttaatg acttatataa aaggagtaaa aatggcagag
     4381 attctacagc gaatatttta aaagaactta tagaaaatgg atatataaca agaaaacctt
     4441 gtagagattc taatactaat aaaatgcttg gagggtatga ttacgaagta tatgagatac
     4501 cacttgaaaa tccacaaaaa ttaaaatccc gaaaaactga tttcccggaa accggatttc
     4561 ccggaaaccg ggtttctcgg aaaccggaaa acacggaagt agtaagtaat gactttaaag
     4621 taaataatga tattactact attgttatta atgaacaatc caataaagac aaaaccacct
     4681 acataaaaaa atactttgaa aaatatatag gtgtgattac tcctaataac tttatagagt
     4741 taatgagtta cttagatgat ggaatggaag ctgatgtaat tataagagct attgatgaag
     4801 caatagcaaa tggagttaag aattataagt atgtaaagac aatattaaat aattggatag
     4861 aagcaggtgt aaaaactaat ttagaactta cagagtatca aaatgagttt gagaggaaga
     4921 aaaagaataa acaggataag aagcagtcta atagtaaagc tgtgaatact cctaatgtga
     4981 gtaaaaataa gtttcataac ttcaatgaaa catttactca atattcacct gacgaactag
     5041 atgacataat taagaaaagc caaaaggtta aatttaaata aaattaaact tctaggaagt
     5101 aaatatcaat atattgcttc ctagaaaagg ggaggtataa aatggcgaga atatatgcac
     5161 aaagaagtgg ttctttaaat gaacaagata gattggagtt attaagatta cttgggaaag
     5221 ctggatatac agtaaaggtt gctagagaga agcaaaatag caagacaact tatacttact
     5281 ttgttgagta tacagaagaa caggaagaaa aatagaaggg ggctagttaa atgaatacaa
     5341 taactttagt tggaagatta gttgcagatg cagaattgaa gtaccttcca aattcaggta
     5401 ctccaaaaat aaccttttca atggcagtag atagaaggtt taaagataaa aatggaaata
     5461 aaataactga ttttattcaa tgcgagcaat taggaaaaca tgtagagaat ttagtgcaat
     5521 atcttgttaa aggtaagcct atatatgctg ttggagagtt aaatatatat aattacaaag
     5581 atgaaaatgg ttgctggaaa tctattacta aggttaatgt aaatgcttta gaactacttt
     5641 ctagtaaaaa tgataataat gctaaacaag aatatgtacc accaggatta gacccacaag
     5701 gttttcaagc aatagatgat gacgatatac ctttttaatt aagttaaata gtctagggag
     5761 taattataca atattacttc ctaaaagtta aaaatggagg gataaaaatg agtagaacag
     5821 ctatatgtag tgtttgtggg gagtttctag ttgagagtta tgatgaccag tttacaaata
     5881 caaaggttat ggatttacat ggtagagaat ttgtaattgt tatatgtaag agttgcttag
     5941 aagaattatt aagaaaatgt gaggactaac aatatgagat ttgagatagg taaaacttat
     6001 aagtttgata aagagaaatt tatggaaatt aatggtgtag aacaacataa aaaatataaa
     6061 gaactttgga ttgatgatat tgaaggtgtt gaatttactg ttgaaaaaac ttttgatgat
     6121 ggctatgttt gctatccaaa tgaattttgg tttaattttg gtgtagtttc ggaatggtgt
     6181 gttgaagtta aataaggtag gaggaattag atatggctaa aatttggtta gatgcaggaa
     6241 cgtttttaga aaaaactatt gatatagaag atatgtttga gcttaattta agaaaagtaa
     6301 gaaaagcaaa tgaaagtaag aaaataaagc tgaaacttaa tgaatcgaaa ttcaaaaaaa
     6361 taaggaaaaa accaactaat gatacaaaaa gtaaacctat aaaagttttt aatattgaaa
     6421 ctggagaagt tagaatattt aaaagtgcaa aggctgcaag taaatactta aagattagtg
     6481 gagattatgc tagttgttta gctagagaaa atagagtaac tagagaaggt tggaaggcag
     6541 aatatattca agaggtgtca gatggtatta gcaaatgtgg agcaagtaat taagttagct
     6601 gaaaagatat taaataagaa aaagtgttct gttaataaag ctattgatat agctataaaa
     6661 atattaagca aatatgagtg cgaggggatg attagaaatg agtctaatta agtacagagg
     6721 ttatgatttt gagaatgaga agtggattta ttctgaaaca ataaagtgga gtgatgcagt
     6781 agattgttta tttatgctaa aagagaattg tgaatggcaa aaagtatcta atgttggagt
     6841 attctcgaaa tgttggtctg gaaataatga agaaatttat gagggagata tattgaaaga
     6901 accctataat actaaaaata aatatgaata tggaattgta agacaagaaa attatgctct
     6961 tgaattatat gttgaatggc attatttaaa gcaatttgaa ggtaaatggg aggaaattac
     7021 aagtaaagca actataatga atagcaaaaa atatgtagta gttggtaatg aatgcgagaa
     7081 tttggaagaa attaaaaaag agttcttaga acgtaaggag atgttagaaa atgaacatct
     7141 tagctagttt aatacttata gtaggaagtt ttatagctgg tagggtttat gagtatagat
     7201 tgaatctaaa agagtgtgaa aattgcgaca ataaaagagg tgtataagaa tggatgatag
     7261 attagaaatt ataaatgctt ctgtaaatta tatacagatg atatgtgaaa gttcaaatat
     7321 agctattata gcagagcgag gaagagttag aatattagat ttagaaacta aagaaaaata
     7381 tgatttatta aaaaataaac tcgaggaaat gttagaagaa atataagtga aaatatctaa
     7441 ttaagacagt ttagagagtt gcaaaatgtc ttttggtata aattattgtt gaagtgtttt
     7501 gtgactctca aaaatgaaaa tagagagggg aataatataa tggatttaaa taaaattatg
     7561 aatgatgcac tagtagagtt agaggaaaat ggctttgtag aggaagtagt taaaaaacaa
     7621 ttggcagaaa caataaaaag agttgtgaat gaggtttttg gaaattgggg tgattttagt
     7681 aaaaaaattg aaaaacatct aagtgaaaat ataaatataa atttagataa attagatata
     7741 caaaaatata atgtgctcgt agcaactaca gtaaaagaaa agattgatat gacaatgaag
     7801 gtagaagcta ttgaacactt aaaaagaaat ttagataaca tgcttgtagg tgctgaaaaa
     7861 gaatataaaa tgagcgaatt gttggaaata ttaaaaatgg ataaatatga tattgatgaa
     7921 tatgaaggct atgaagattg cataacattt attcttgatg aaaataaata tggttcggga
     7981 tggattgaaa ttgataaaga accaaataaa agtaaataca gatgcgaaca ctccatattg
     8041 ttgagaaaag atggaactat tgcatcatat agatacgaga atagagaaat atcatctaaa
     8101 gatataatga gtggatttga taattttgga gatttgctat ttaaaatatt tgctcatggg
     8161 tcaaaaataa ttttggattt aggaaatgat atagaagatt atgatttaac aaatggagaa
     8221 gattattaaa ataggggatg gaacaaatgt caaagtatgt attgagatgg caaataggat
     8281 tatcgttaga aaatagaaaa atacattata catatggaag taaagaagct ttaagaaaga
     8341 aagcaaaggc attggctaaa gatgaaaata tagtactaat aactatagat aaggtagacg
     8401 aagttataaa aaatactata agcgagaaga ttatagaacg ctttaaaaat ttataagggg
     8461 tgaaattatg ataatacaca aatttataat acatgtttta gataagaaca gcgatacacc
     8521 aatactaaat gattttgaag gtagagttag tcaagatatg gacctatttt ttcaaaagaa
     8581 aataagcaaa gtatcaagag ataatgacat cagaacagca gtatttaatg actatagtaa
     8641 caatctaatt aagaagtgtt gtgaacaaat tatttatgat gaaagttcat ttttaaataa
     8701 ctctaaagag attgcagctt atttatttga tgttatgaaa ttgaatgcta cattagaatc
     8761 ttgcgactta gcaatttgct tatactctca aaaagatgaa aagaaagttg ctatattaaa
     8821 acttgattac aataagtcat atactcattc aattgagttt gaagatgata aatttaatat
     8881 acagatgtct aaaaatgaaa ttaacataca agagactaag atagtcaaaa ttgccgtcat
     8941 agttggatta agtggaatga atgacgaata tcatctaaaa gtattggata aggatgcaga
     9001 aaaggaagaa gctaattcta agtttgttac agagttctta aatgctacta agataaaaga
     9061 tgataagtat aagactaaga tgtttaaagc ttttgtggac tcttatatag cacatttata
     9121 tagtgatatg aagcagggcg aagatgtaag aggaatgtta ctttatatgc taagagaaaa
     9181 acaaaaactt gatataaatg agtttgctga taaggcgata aaggatgatt taaaagatag
     9241 ttttaaggac catatagaag aaaaagggat tgaaagtttt aatattgata aaaaatgggt
     9301 tgaaaagaat ttaaaaaata gacatataaa aacagataca ggttttgaga taaaaggcaa
     9361 gatggatgat tttgaggatt ttatgaagta tggtattaga cataatggga atggaactgt
     9421 agatatagtt attaaaaatg ttaatttcta taatgagaag taggtgctca tatgaaatat
     9481 gtagctagtt ttagtggagg aaaagattca gcagcaatgc tccttttgat attagaaaag
     9541 aaattacctc ttgatgaaat tgtatttata gatacagggc tagaatttga agaaatttat
     9601 gacataatag atggttttga aaaaagaata aactttaaaa taacaagaat taaagcagaa
     9661 aaaacctttg aagaatactt ttatactgtt aataaacaag gtaagcataa agggcaaata
     9721 tggggttttc catacacctt gggagcatgg tgtaatagtc gattaaaact tgctcctgct
     9781 aataaatatt tcaattcgat tggagaacac aagcgatata tcggaattgc ctttgatgaa
     9841 cctaacagat ataaaagact ggaaaaaaac tgtatagcac cactttacga agcaaaaatg
     9901 actgaaaaag attgtttgaa atatttagaa gaaaaaggat tttattatga gatacatcac
     9961 agattcaagc gaactggttg ctatttatgc cctaaacaaa gcttagatag cctcagaaca
    10021 ttaagaaaat attatcccga tttgtggggg agtatgctta aattagataa agatagtcct
    10081 gtacctttta gagctaatgg ggtcacagta catgacctag aaaaacgatt tagtaatgaa
    10141 gatatagaaa atgaaagaca gataagtttc tttcaaacag agaaggtgag catatgacta
    10201 ataaagaaat gtgcaagtca aataatcttg atgaaagaga agtgtataag aaatttggga
    10261 aagagatttg tggtagttgc ataaatgata aggtagattg cgaaagcaaa gattgtgata
    10321 caacatataa aaattggtta gaaaaggaga tttaaaaatg aaaataaatt taaatgatat
    10381 tccagaagga tataaagtag ctgttattcc tatacttgag gctataaaga gagcgaaaga
    10441 agatgaaaaa gaattaagag aatatttaaa ggaaatagaa gagtgtaaaa aaataaatat
    10501 atcaattgta agaaaaaatg gtactaaaat accgcttact tttacaaata aagaattaaa
    10561 tattcgctta aaagaagaaa ttaaactagc tattaaatgt ataaaagaag aagctactga
    10621 aataaaggaa ttattaatta aaatggaaag tgaaggaaac aaatagtcta ggtttaaata
    10681 atttaagtag tcaaggtaag tttatgaatg aaactagaat gttatagact tactttgact
    10741 tataaaagag gtgcgattat gattactact caaatgtata agataaataa tgagatatat
    10801 ctaaataata tatgtttgga agaaaataag gaaattacca tatgggtaga aaagaataac
    10861 acacatgaac ttatatggtt aaaaatagcg aatgtaaatg gaaagttagc tatatttgta
    10921 caagatattg aaaatgctgt aataaaagaa tggcaaggtc gtaaagaata tgaaagagtg
    10981 ctaggacaaa tagaagataa acaagttgaa aaaggggaaa tattgtatga agaatactgg
    11041 aaataaagtt gagataaaca acatacgaga gttaaatgat gctttaaata agtatgatat
    11101 cccatttggc atattaagtg atgtagatag aagaatatgt gactggatgg ctactggtgg
    11161 taatgaagat gatgcttata ttaaacaaca atatagatac gtagagaatt ttataaatag
    11221 attttgcgac tagggagtga gattatgaaa aggagcagtc aatatggtta ttacagaatt
    11281 taaagtgact tggcagacaa caagagaatg ggaaaaaggg aacgtataca gctctatatt
    11341 tactgactta gaagaagcaa gagcttttaa aaaagatgag gaaaatataa aagaaaatta
    11401 taatgtgaag cttttaagta gagctatagt cgaaattgaa atagattaaa tatgtgagtt
    11461 aaaaggaagt gattctttga aaaggataag atgcagttgg tgtggcaaat tattttatct
    11521 taaggaaaaa tctaaggatg tttattgttg taaagaatgt aggaaaaagg ctaagaaggt
    11581 gaaaaaatga aagtttttct tgtaatagat ggagaaccaa ttggtaaaga aagacctagg
    11641 tttattttgg ctactaaaag gacctataca cctaataaga ctagagatta tgaggaacta
    11701 ataagatggt tatatcaatc taaagttaaa cattactttg aaggttatat aaaaatgact
    11761 ttaagatgtt actattctat agctaaaagt aatagtaaaa aggttaagga gcagaaaaga
    11821 aataatgtgt taagacctag taaaaaaccc gatattgaca atgttgttaa gattatagct
    11881 gattcactca atgagatagc ttataaagat gatacgcaga ttgttgaggt tgtagctagt
    11941 aaatattaca gtgataaacc aagagttgag gttatattag aagatgttat ttaaccaacg
    12001 gaaaaatccg ttcgttagat taggtactca gtttttcata aaaatgtgaa aaagctgaat
    12061 agagaatata tcaaatggta aaggagagat aaattatgaa tgaaaatata aataaagaaa
    12121 taacagtact tggaacttta gaaatcgagg gaatgaaatt tcataacatt gagggtggat
    12181 ttggagaaca taagaaagca atgctagtaa aagatatagc tgagatacat aatagagaat
    12241 ctagacagat taatgagctt ataaataaga atagaaaaag atttaaagat ggaaaagata
    12301 tattagattt gttaggtgtc ggtttggacg ataccaaaat aaaagaatta ggatttactc
    12361 aacaatcaat taattcttat agagggttaa aaaacaaagg gttattatct gggatttata
    12421 tattatctga aagaggttat gcaaaattat taaaaatatt agaagatgat atagcttggg
    12481 aattatatga gaagttagtt gatggatatt tctctatgag aaaagaacta aataatcctc
    12541 ttttaagtgc atcaaaggag ttacaagcta tatttatgct agataagaaa caagaagtct
    12601 tagaaactaa aatagagaat gttaatgaga agttagagaa ctttatggat gatgcaccat
    12661 tattcaatat cgagtgtgag tgtattgtta aagaggttaa gagagtagca acaaaatcac
    12721 ttggtggaca tggaagtaag gcttataaaa ataaatcttt aagaggtaaa gtttatagtg
    12781 atatatacca tcagattaaa cgagaatttg gagtagatag ttataaggct ataaagcgtt
    12841 gtcagttaga taaagtatta gagattgtaa atagttataa gttacctata gtgtttgaag
    12901 aagaaataag acttttgaat agtcaattat caattgtgag ttaagacagg aatagatttt
    12961 tctcctttaa aggggaaaac ctgcaatagt tagttaaaag aaaaaaggag tgctttcaca
    13021 ctccacttgt caaaaatata aaacttttat atacaaatat tattataaca taaaaaattg
    13081 ataggagtgt ggaagtatga ataaaaagac actatttcaa gaggttgaag gtagattata
    13141 taactataaa aaactagaaa gtcaaataag aataaaagat atatatatta aaaaattaga
    13201 gaatgaattt tgcggatgca aggctcaaag ttatgaagaa aaaacaggaa ctacctataa
    13261 tataagttca agtgttgaaa atgaagttat taaaagagaa gaagatttaa atagattaaa
    13321 agaagataag aaaacattag aaattgaaaa agaaactata gaatgtgcat taacaagttt
    13381 gaacagcttt gaaacagagt tcttcaatga aatgtatatg aataatgaaa agataaatat
    13441 ggattatatg tctaatgcta tgcacataga tagaagtcat tgctttagaa taagaaaaag
    13501 gatagtttgt aagattatgg atatgttata tccaaagata aaggagtttg aattacccat
    13561 tttttcatgg aaagcttaaa aatgagacta ttttgagact ttttggagac tatcttgaga
    13621 ctttttattg gtaaaaacat gagataataa tattgtggaa atgaagattt ccctctcaaa
    13681 attaaatata tattaggcta gagttaaggg attgtctttg ctctagccaa agtgaataat
    13741 ttttataatt aaggacatca tataaaaagt tgaaaaattt tcaaatgacg ctttaaatat
    13801 aaaaaataaa tatatttgtc ttttgatatt gacactttta cataaaacgc ctacaataaa
    13861 tatataaaaa tatataggag gtgtatgcct gagtgtcaaa taaaataaaa ttagaagttc
    13921 ccaatgaaat aggttatatc attgttcaat gctttaataa ggcaaaagaa gaaattagag
    13981 aatatatagg aaaacaaaac cttataacta aaaatggagt gccttttcag ttttgggatt
    14041 tgttaaatga tgcaattgat actgcattta atagttctaa ttttaaagcg tataaatcta
    14101 aacgtggacg ttggagaatg atttttatat atgataaaaa tagcaagtat ctttatgtat
    14161 taatgagaga gcaaagatat aaagaacttc ataataatgt caataaaaga gaaaaaatgc
    14221 attatgtaga tattttaact aaagtattta atggagattt aaaagctcct attgaacaac
    14281 tatcattttt ttcattagaa tttgataatg aagataaaat tgaaatatat ttacagaaat
    14341 taattgatag tataaataaa gatggtgcta tcattgaaca ccatgtatta ttgttattta
    14401 atcaaacagc tgaatatgaa ttgacatcta ttcgtgctgt gatggttgac acaaatttaa
    14461 atattgtatc agaacaaaat ttgagttctt atatatctat agaagaaagt acagttgtag
    14521 aaaaagttga taagactaat cctgttgcaa ataatcctac tcttggattg aagttaactg
    14581 caaaagcaga tgcaagaaag aaaacacatc ctttaaaaaa ggcgaaaaag gaggaagatg
    14641 ccaagtagtc actttttagt gaatagatgg aggaaataat tatggaaatt aattttaatg
    14701 gagagcgttt aaagaaagct cgtatatata gaggaatgac agtagctgaa ttagctgaga
    14761 gaattgactg tcaaagacag actgtttcga tgtatgagaa taataaatcg aaaccaaatg
    14821 ataataatgt agtgaaacga atagcaaaag agttaggttt cccagttaaa ttctttttag
    14881 aaacaggaaa taatattgca ataggttcaa catatttcag agcattattg actacaaata
    14941 aaaaatatag agcagagcaa attcaaaaaa tggaatttct agcagaaata tatttcttct
    15001 tacaagatta tattgaattc ccaacattaa atttaccaga ttgttctggg aaaacaccag
    15061 aagaagctgc tttactttta agagaaactt ggggattagg attaaaacct attgataata
    15121 tcatatatga agtagaacaa cacggaatac ttgtaacaag tttctcgact tctacagatg
    15181 atatagatgc attcagtcag atggtagata tttcgggaga gacattttat ctgattggat
    15241 attctagtaa taagacttct gcctctagaa ttcattttga tatagctcat gaattagggc
    15301 atatatgctt gcatgagtgg agcgaggatg tagaagcttt agaaaaacaa gaatttaaag
    15361 acagagaatc agaagctaat agatttgcat ctgcattctt attaccagaa gagactttta
    15421 aaattgatgc taagagaact cctttacgta ttccaaacta tacagaattg aaacgtaaat
    15481 ggaaagtttc tattcaagca atgattcgtc gttcatattc tttaggaatc atcagcatgg
    15541 atgaatatca ttctatgatt cgtactttac aacgtagggg actaagaaaa tcagaaccat
    15601 tagatgatga attattaact tctctaccag cattgttaaa aacagcagtt ttgatgttat
    15661 taaatgagaa ggtatttact ccaaaagagt ttatggatga actttcattt tcctataact
    15721 ttagtctaga gccagaagaa gtagaatatc tattaagttt acctaaaaat actctaactt
    15781 ctgctaaagt tataccattt cctgatttac aacttaaaaa agatgtttaa atcatatttt
    15841 aaatagagcg aggacttatg acctcgctta aattatatag aataggggga aatatgaaat
    15901 gggaatatta gaaggtgcaa ctaaaataag agagatagcc aaaaagattg ctatagaaaa
    15961 aggaataaca gagcaagagg catggaatga tgctattaaa gagtacaagg aaaaatatga
    16021 atttaattaa gaccaatgta tggtcttttt tttattacta tgaacagact aggtagggcg
    16081 tgaggacgct gttagttcaa ttctaactat gttcaaatat taatcaacgt atacactaaa
    16141 agtagagaaa ttgagggcaa aattttatat tttgtatctt aattcagagg tctaaaaaac
    16201 gagtggggct tggtaacctc actcaccatg caagtactgg tttaatctag gttcgattcc
    16261 tggaacttgc tccctttaat aatatgtatc ccccattaaa aaggcttaga ttaacttcta
    16321 ggtctttttt aatacaaaaa tttattaaaa atgcacggca tgcactattc agatatgcat
    16381 gtcatgcatg tctaaagtcg aatatttaat attttcgatg gctcaaattg aggggtcgaa
    16441 aataaaaaca ggaggtagta gtatgttgaa aattttacaa gagaaaaatg taaaagtaat
    16501 gtggtccaaa aatggagaag aagtttggtt taatgcaaat gacgtaggag aggaactagg
    16561 catagtaaat attcgtgata cattaagaaa tatagataga gaatataaaa agaaatttaa
    16621 tgagtctact gtcggagatt cctacactag aaactttaaa gataaattgc ctaacttcgg
    16681 tactactttt gttacagaag aagctgtgta caatatgtca tttagaagta ataaaccaga
    16741 agcaaagtta tttacaaaat gggttacaaa aacacttaaa caaattagaa tacatggtta
    16801 ttatattgct acagaaaaag accaggaatg gctggatata aggacagaag gcaaaaaagt
    16861 aagaaaagat tttacagatg aaatacaaga gtttgtatat tatgctacta gtcaaggtag
    16921 caataaacct cagatgtatt ataaacattt tactgaactt gtaagaaaaa aattaggtat
    16981 tccaaaaggt gtgaaaagag atgagttaaa tcaaagcgaa ctatttgata tacaagcact
    17041 tgaaagaatt atatctatga aattacctaa gttaatagat aaagatatga attataaaga
    17101 ggtatataaa aagattaagg aattaataga aatgatttaa gggactgtct tgatggaggg
    17161 tcttttttaa ttcccaaaac gacaaacaaa cgaggtggtg atgtgcaaga tgtcaaagaa
    17221 aaggtaaaac aagattacat aaaaggtatg aaacaaaagg aaatatcagc aaagtatgac
    17281 attagtttaa acactttaaa gtcatggata aaaagataca attgggctag tgagaaaaag
    17341 aagggtgcac ctaaaaataa aagaggtgca cccataggta ataaaaatgc cactggtcct
    17401 cctggaaata aaaatgctga aaagtttggt ttcttctcaa aatatctacc tgaagaaacc
    17461 caagacttaa ttaatgaaat aaagaataaa gataaatttg atattctttg ggaacagata
    17521 acaattcaat atgcagcaat aataagagca cagaagataa tgtatgttaa aggcaaggaa
    17581 gaaatggtta aggaattaaa gaaacatgaa agcacagaaa atggtgagaa gatagagtat
    17641 gaatttcaat ttgcatggga taggcaagca tcttttctta atgcacagag tagggcaatg
    17701 agtgaattaa gaagtcttat taaacaatat gatgaaatga ttcataagga ttggaatttg
    17761 gctacagagg agcagaaaaa tagagttgag aagttaaaat gtgaagttga taacctaaat
    17821 aaagatgata cagacaaaga aataactgta agagttatga aagcgagtgg agaaaatgga
    17881 aagtaaattt atatctaatg accattttta tgattttata tttaactggg attacaaatt
    17941 ttattttttg gttggaggat atggaagttc aaagagttat catgttgcaa ctaaattaat
    18001 cattaagctt cttgaagaaa aaagaaaagc tttagtggta agagaagtat atgacacaat
    18061 gagagatagt tgtttttctt tattagaaga agttgcagaa agtatgggtt taggatatat
    18121 attatcattt aaaacaagtc ctatgagagt tatttttcct aatggaagta agataatttt
    18181 taaaggaatg gataatccag ctaaactgaa atcaataaat ggtgtatcta taatatggct
    18241 agaagagtgt tcggaagtta aatacactgg ttttaaagag cttttgggac gtttaagaca
    18301 ccccagttta tccaatcata taatttgttc aactaaccca attggcgaag ataactggac
    18361 ctataaacat ttttttagag atgaagaaaa agacagattt gtattagatg atactgtgtt
    18421 atataaaaat agaatagtaa caaagaataa tacatattat catcattcag ttgcagatga
    18481 taattttttt ctgccagaaa gttatattga acagcttgat gaaatgaaag aatatgacta
    18541 cgacttatac agaatagcaa ggaaaggaag gtttggtgta aacggaacta aagtattgcc
    18601 acagtttgaa gttatggagc atcaagaagt tataaataaa atatccaata taccacaaag
    18661 atattataaa gttggattcg actttggatt tgaaaaatca tataatgcac ttttgagatt
    18721 agctatagac catgaaaata aatatttata tatttactgg gagtattata agaatcaaac
    18781 tacagatgat gtgactgtta aagatattaa agaatttaaa gatactcaag aactaataaa
    18841 agctgattct gctgaaccta aaactataaa atattttaga caacaaggtt ttaatatatt
    18901 tggagctaaa aaatatgctg gaagtagatt acaaaacact aagaaggtta agagatttaa
    18961 aaaaatttat tgttcaagtg agtgtaagca tacaataaag gaattaaaga acttaacata
    19021 tgctacagat aaaaacggaa acttaatata tgatgaattt aatatagacc ctcatacatt
    19081 tagtgctata tggtatggac ttgatggata tgaggtatca gacattaaag agttaaaata
    19141 tagtaatgat atttataaca aaggtttagg attgaagaaa aataatcaat ataataagaa
    19201 aggagggact gtattttagt gaatataaaa aatatcttac tcaatttaga tgaaaaagag
    19261 ttaagagata gaaagcatgc agaaagagat ttcttatttt atttgggtga atgtagaaat
    19321 aaaactatgg gattgttaga tgatgatttt ttaggtcaaa gttggattac ttttgataac
    19381 ctagactata caccttctca aattgtagat aataaggtaa aaccacttat aaataaacaa
    19441 gctcgtttta tgtttggaaa agagccaact ataatactta aagcatatga aaaagaacat
    19501 aaagaagcat gtgaggaatt aaggcagtat attgattcaa tattaaatgc cagcaagttt
    19561 tggagcaaca cactaaaagc ttttaagata gcaactataa cgaaaagagt cttattaagg
    19621 ttagaagctg aaccaaatca acctataaga ctcttttatc attctataaa tgatttcaaa
    19681 tatcaagttg atagtaatga tattacaaaa ttaaagtcag ttgtatttgt tagatttgat
    19741 tcatcaacta taaaagaagt aacagcaaaa caaatatggt atagatatac ttactatatg
    19801 aaaaaaagtg atgtcagtaa tcaagaaagt tgctttataa agatagaaaa gttcaaaggt
    19861 gataacttat ctaaacctat taaaataaca gaaaatgata ccaagctttc taaaatacca
    19921 tgttgggtga ttgttaatga acaaagcatt acaaatataa gaggaattag cgatattgag
    19981 gacttaaaac ctttacagga cacttataat aaaaggttgt cagattttaa tgattcttta
    20041 aaatttctaa tgtttggtca aacagttgta gtagatgcga cagaagaaac agttaatgct
    20101 tgcaagattg ctcctaatgc tttaatggca ttaaaaacac ttgaagaagg ttcagaaaaa
    20161 tcaaaacaag cacaagcgta tagggttgag agtagttttt caaatgcaga ccctgtaaac
    20221 tcttttttta aaagacttga agatagtatg tatgaaaaat tagcaatacc tagacctgaa
    20281 caattacaga atatacctag tgccaaagct ttgaaatatt tatatacaga acttattgca
    20341 agatgttcag aaaagtggaa tgattgggaa cctgcaataa gaagcatgtt aagattaata
    20401 gttgaagctt gtagtaaatt taattgttat gatgattgga atcatgattg ggatgattta
    20461 atgttttcta ttgtattaaa taaaaattat ccaattccag aagatgaaga agattcaaaa
    20521 aggttggcgc ttgaagaagt taataataat gttagaagtc atagaaatta tataaaagaa
    20581 tttggagatg atgaagatta tgaggaagca tttaatgaag tactagaaga taatgaaaag
    20641 atacaatcag tagagcaaga ccagttcaga aaagatgcag aaatagaaat tgataatatt
    20701 gatgaagaat taaatagtaa atctaataat agtgatgagt aatgaggtgt tgttatgaag
    20761 gacaatactt atacaaagaa agttcttgaa gttagaaaaa aacttttatt attagataag
    20821 aaagtacaat tagagatatt aaatgtctat aaagatgcta gtaaaactat tttaagtgat
    20881 attgctaaaa ataaagagtt gaatctaagt actaaatact taaaaaagct aaataaatca
    20941 atcgagaagt atatcaatga gctaaatcaa agattagtac ctgttacaga aaaaagtata
    21001 acagaagctt ctaatatagc taaagattta caaatgtatt actatcaatc aatagttcca
    21061 aataaatcta taaatcttgc atgtgatgct atgtgtataa atacaactac taatgttgtg
    21121 gaaaaaatag ttgcaggtaa cttttataaa gataaaaggt cattagatgg cagaatttgg
    21181 ggctataata ataaaaatag aaaagatatt gacagattaa taaaagctaa tattgcaaga
    21241 ggtgctaatg caaagacgtt agcaaagagt ttagataatt atgttaatcc agtcaaaaaa
    21301 acagaagcaa agacgttaga agttggaatg aataaaagta tatcttatca agcccaaaga
    21361 cttgctagga cttctataac acatgctttt gtagaaacaa gtgttcaaaa tgcaataaat
    21421 aatccttttt gtgtaggatt acaatggaac ctaagttctc aacattatat tagacaggtg
    21481 aaatggagag gcgaggatga gtgtgatgag tatgcagaac aaaatcgttt tgggctaggt
    21541 gaaggagttt tcccaccaga aaagtatcct attccacacc ctaattgtct ttgttatccc
    21601 actcaaatta tagtgccaat caatgaagct tcaaaattca tgaatgactg gttaaatggc
    21661 atggataata gcaatttaga tagttggtat gatgaaatta acacagaaac tactaatcta
    21721 tatataccta agataaaata ttcatctaat gatgtgaaaa gtaagtatgg aaaaacttta
    21781 aataaaaaga ttaatagttt tgaaagtaaa atagaaaata catctagcaa atataaaaaa
    21841 tatacaactg gtgttaagta tgaggtaaca aatgagttaa aaactgcttt tgcatactca
    21901 ataaaagatg atataattaa gataaatcct ttaagcccca tatttaaaga gtataatttt
    21961 gaattgtcaa tgatgcatga attaggtcat agagttgatt taaaagaact tgaaatatat
    22021 aaaaatattg aatttaaaga tgctatttta aaatcatcca attatgtttt aaataatctt
    22081 aaagaaatac aaattgaata tgattactta gtaaatataa atgatagccc ttttttaagt
    22141 gatatattag gagcattatc atttaataaa atcgaggggc ttttagcaac tcataatata
    22201 acctattggc aaaagcaagg taatatagaa aaagaaatat ttgctaattt atttgcttta
    22261 gattatgaaa gtgacaaaca tatattaagc tttataaaga aaaatatacc tgacatatat
    22321 aatgtattca ataaagttat ttaggaggtg tgcattgtgg gtgaacaaat gccaataatg
    22381 aaaaggctta gaaatgataa agaactattg gaactaagaa gattatataa agaaaaatac
    22441 aatgaaaatg ctcccggatt taattatgat gaatattcta gttatgatga gtacaaagaa
    22501 aagctaaaag aattgataca aaagtaaagt aagcacttat taattaaagg ttaatgagtg
    22561 cttttattat gcttaatttt aaaggaggaa attgaatgtt agagtatttt aaaaagttac
    22621 ttggagatga agaagggcaa aaagtttatg aaaaattatc taaagataaa gaaaataagc
    22681 ttcttttaga taatatcaaa agtcctagat acgttgaaaa gacagaacta gaaaatgcta
    22741 ataaagagat taaagagtat aaaaagcaaa tgggggatag agataagcaa ctaaatgatt
    22801 tacaaggcaa agttaaagat aacaaagagt tatcagatga aattgaaagt cttaaaaatg
    22861 caaataaaga aattagagaa agtgcagaaa aagaaattga ggttttaaag tttaatacag
    22921 cttttgaaag agttattgaa agttataatc ctagaaactc aaaagcttta gctgctttaa
    22981 taaataagga taatattagt tttgtaaatg gtaaatttat tggattagat gaacaaataa
    23041 aagcttatca acaaagcgat tcttatttat tcaataatga aaaaaacaaa ggagatgatg
    23101 aaattggagg aactggagga ttagaaggcg gaacaacttc attacttgat aatgatagcg
    23161 aaataagtag tataggtgag ttgttagcta actctaaagt aaaaagcgaa aatatagaag
    23221 ctcaaaagaa attttttgga gaagaatagg aggaaaatat atgagtatag aaaaatccga
    23281 gatttacatg ggggaaaata aaactacatt aaaatttgca ggaaacttat ttcaaaatgt
    23341 aaatattaaa gtgaaaaaaa ctgatgtagc tatagtagat ggaaagagaa ttttaaaagc
    23401 gggtacatta gtatcaaaag atggaaaatt agtagatggt acaactgtta caaatgataa
    23461 agcttttggt ctagtatata gaaatataga tttcacttat tcaaatggta atgaaagcat
    23521 tccagtacta atatttggat ttgtggatga aaagaccttg ccatcagtta taccagaaga
    23581 agcaaaacaa gcaatgaaaa tgattatgtt tttataaaat aaatattagg aggaattaaa
    23641 atggattgga aagactttat agactctaag gagatagcta aatatataaa aaagttgcca
    23701 ctagaaatgt taataggcga atctttgttt ccacgaaaaa agcaaatagg tatggacctg
    23761 aaatatatta agggagcgaa gaaaaaacca gtagtgttaa aacaaagcac ttttgatgtt
    23821 gctgtaaaaa taagagcatt aaaagctcaa atagaagtta aatcaaagag aatgccattt
    23881 tttaaagaga gtgttcttgt aaatgaagaa gatagacaac agttactttt agcttctaaa
    23941 gctcaaaaca aagaactatt attaatgata atttcacaaa tatatgataa ttatttagct
    24001 cttgtagatg gtggcgatat gcaaatggaa agaatgagaa tgcaagcttt agctgatgga
    24061 gtaataaata tcgtatcaga agatggagac ttagtatttg actttgaagt tccaagcaat
    24121 cataaagaag tgttaactgg aagtgcaaca tgggataatc cagatgcaga tattatagga
    24181 gatattcaaa gatggatgag aataatgaga gatgaaggga atccgttacc taagagaatg
    24241 gtaatgacaa gtaaaacttt tgggtatttt gctaaaaata aagctattaa attagatata
    24301 gataaagatg gtagagttat tttaactgat gaaatgatta aaaattatct taaaaataaa
    24361 gttggtttat ctgttgccat agtaagtgga acatataaat tagaagatga aagtgaggaa
    24421 tcttatttcc ctgataacaa aataactttt attccagatg gagatttagg aaaaacttat
    24481 tatggtacaa caccagaaga agctgacaaa gtgtatggtt ctaaattaga ttgttctgtt
    24541 gttagaactg gtattgctat aacgacaatg agattaattg acccagtaac agttcaaacc
    24601 aaggtatcac aattaggtat gccaagcttt gaacgtgctg atgaatgttt ctttgctaca
    24661 gtagcataat taaggggtga taattatggc taaaaagaaa gagaatttaa tgcaagtaaa
    24721 agctttagta tatctaaagt atgataatga atgttataaa atagatgata tatttgagat
    24781 tagaaaagct gaccaagagc ttatggaaga aaaaggatat atagaagtta taggagaagt
    24841 tgaggaaaaa aataataatg agcttcttaa taaagatggt gagtagatat gtctattact
    24901 gatttagata aactaaaact caatttgcaa gaagaagagt atccttattt tacagatgag
    24961 caacttgaga tgttattgga atctaatgag aataatgttt taaaagcttc ttggagaggt
    25021 tgtttattaa aaggtgctac agatgatgaa ataaaaatag gtcctattga aacgaagtct
    25081 agtaacagct catattggtt gactctagca gatatatata aaactgatta tttagaagaa
    25141 aaatcaaaga atgaaacaac tattacagga tataaaacgt ctatgacaag agctgatgga
    25201 caatgagaaa attaagagct gataagataa taaagactat taatcgagga atagctttga
    25261 atcctcaaac aataactata gagcaagaag tgaaaaatat agtagatggg gctattgata
    25321 ttacaaatga agtaaaaaaa ctaacagtag ttatatatcc cgaaaaaact aatgatacag
    25381 taataaatag tgaaactatt ggcacagctt ataaaaataa aaactttggt atggttgcag
    25441 ataaagaagc tgatttaaga ttgaacacag aaaatgagat aacttttaag tgtattgaag
    25501 gaactatgaa actaaactat gtaaatccta ttgtagttga ggaacaggtt tgtggttata
    25561 tctgtggtct tgaaaagcta gattagaggt ggtttagtat gagtgtgttt actaaggcta
    25621 taaatgaaat tgatagaaaa aaggctacaa tgccaatttt gtgtatgaat atagctttta
    25681 tgttagaagg agaagctaag aataatgcaa aatggactga taggacagga aatgcaagac
    25741 aaggcataac aggaacaagt ttaggtggag gaaatcaata cattgttagg ttaggtcatg
    25801 gcgttgacta tggaactgtt ttggaagaag gttcagcacc tcatgttata agaccaagaa
    25861 atgcaaaagc tttattttgg aatggagctt cccatcctgt taaacaagtt cagcatcctg
    25921 gtactaaagg aactcattct ttagaatcta tagttagcaa gaatatgcct aaaataggca
    25981 aattagtaga aggacattgg agtaaataat atgagagcag gaataagaaa agccttaatt
    26041 gagaacatac caagaattaa agattgttat gaaccaactg tccctaataa aaaaactata
    26101 aaaccttata ttgtaattgt ccaaggtgaa gatgctgaca acgaaggcaa tgctataggt
    26161 tttagaagga ctataaatat ttggttatat gagaaaagga ctacatttaa taaactggat
    26221 gaacttacaa aagaagtcgt agaagcttta gattttaaaa ctataacaga tgatacttct
    26281 aatgaggtat ttacttgtat ttatgagggt gcagttggtc aagatgttat agacgaggaa
    26341 tgggaagcta taataaggtg tctaagattt agtgtaatag ctttagaaga taaagaagat
    26401 acaactaatg atagatgggt agaagctcta tctaagcaca caaaagattt attagaaata
    26461 gagagctata aagataattg gaagaaaaac tttatagctc catgtgtatt atggcgaact
    26521 acaaacgtta aaaacaaaag aataaattat catctgattg agattacaaa aactatgaaa
    26581 tgtcatgttg taagtaaaaa caaggatgaa atagttaagc ttcttgaagc attagaaaca
    26641 agtttaataa tagataaaag agtaagactt agagaagata agaatatgta tttaactctt
    26701 gttagcgtag ttgaggatag ggaatcagat atgtttacaa ctggacaatt aacagctgtg
    26761 tttaaaatga taggaaagat aaaaagagaa ggtcctacta tggataaaat ttatggtaat
    26821 ggaaatttaa gataggaggt gcaagggttg gctgaaacaa ataataaaaa gattaatgtg
    26881 agtaagcagg aagaaaaata tttaaaaagt gattttatag aaaatagcga agcacttggc
    26941 tacaagaaag aagtagttgc aggtgcttta tttaattgta agaaagaaga acttacaaaa
    27001 tcagagtttg agaaagcaat aaaagagttt ttagaaagag aggtgaagta aaatggcaac
    27061 tggtacatgg aatgaaaaag aaaaaaagga gatacctggt ttttacaatc gctttaaaac
    27121 acaagcagaa aagtctacaa acacaggatt aaagggtaga ttagcaatgc ctattagggc
    27181 taattgggga gaatttggca aggttgtaac aataaagaat gatttgagac agcttaaaac
    27241 tctattcggg gatgatgtta actattcagc ttacaagtta ggaaaactag ctttactagg
    27301 gaatgtgaag gagctattac tatatagact tgtagatggt aaccaaaaag ttggaacatt
    27361 aactttaaaa gatactacag agaataccgc aaaagatgta attaagctag aaactaagta
    27421 tccaacatct agaaacttta acataacaat aaaatctaat ttagttgatg cagataagaa
    27481 agactttata ttctttgaag ggactaagca gttatttagt tcaagtatca aaggtactat
    27541 agatgaaata gtactagaaa taaactctaa tttagacaat gaatatgtaa ttgcaactaa
    27601 agtagctgat agcgatacaa cactagcaaa tgtagtaaat caagctttag agggtggcaa
    27661 tgatggttgt acatctatta ctaatgagtc ttatttaaaa gctttggagg agtttgaaag
    27721 atatagtttt gactcttttg tacttgatgg tgtggctgag gaagcattgc aggaaactac
    27781 aaaagcttgg gtagctaaga ataaggagct aggaaaagat atattattat ttcttggtgg
    27841 aaaaacagag gataatataa agcaaattaa tgataaatca aaaggtttca atgatgaaaa
    27901 tatagttaat attggaagct cagcctatta tgaaaatata aaatatacac ctagtgaagt
    27961 agctgtttac attgctgcta tttctgtaag taaaggtata acaggaagta tatgtaatgc
    28021 taagactata tttgaagaag tagaaccaag attaagccaa agtgaagtaa aagagtgttt
    28081 gaaatcaggt acactaatac ttgactttga tgatggagat gtaattatag ttgatgatgt
    28141 aaacacattt aagaagtatg tagatgataa aaacgaagca atgggatata tctctaatat
    28201 catgtttatt aatactataa ataaagatac ttcattaaaa agaaaagagt ttgtaggtaa
    28261 gatatttaat gattcgacag gtcaaacaac tgttatatgt gcattgaaga aatattttga
    28321 agaattgatg agtcaaggta ttatatcaga atttaatgtt gatatagata cagagcttca
    28381 agcaactgcc aaagcagatg aattttactg gaagtgggat gctgttaagg ttgatgtcat
    28441 gaaaaaaata tatggtactg gatacttaag ataaaggagg ttatagatta tgtataatga
    28501 tgattatata gaagaagcca gttttctgaa tggttctgat gtagttatac ttattgatgg
    28561 tgtagaagag ctatacatgg aagaaataaa agctgatttt gagcaagatg agcaaagtat
    28621 taaactgtta gggtgtcaaa atgaaatatc aagggttggt actactaaag gttcattctc
    28681 cttgaatgga tataagacag attcaaaatt tgcaaaatta ggatttaggt cttttgaaat
    28741 aatatataat ttatctaatt ctgaaacatt aggatatgaa agtattagat taaagaattg
    28801 tagattaaaa aaattgcctc ttataaattc taaagctggt gaaattgtaa aaatagaagt
    28861 agagggaagt tttagaggat atgatttgtt aaatgaactt taaaacaaaa taatgttgta
    28921 ctttaaagct atgaacaatt aattttgttt gtagcttttt aaaattaaaa atattggagg
    28981 aatagtatgt cagagatata taaaagagag ttagaaaatg aagtagtaga agaagataat
    29041 gatttagatg aagaaataga agaaacaaac gaagatagat tgaagatgaa agaggatgaa
    29101 ataatagcaa agctattgga ggattcacca gttcctcaga gaactgtgtt tttagataga
    29161 ttaggaatac caattacttt gaaagcatta acagaaaaag agattagtaa aataagaaaa
    29221 gaatgcacta aaattgtaaa ggtccaaggg cgaagagaag aaaaattaaa tgatgatgag
    29281 tttactctag cattaataga aaaaggaact gtaaaaccta atttttctaa tcaaaaatta
    29341 cttgatgcta tgaaagtaac aaatgctaga gaatttataa agagaaaatt cttagctgga
    29401 gaattaagta aaataagtga ccaaatatta gaattgtctg gtttttatga tgaaataagt
    29461 gatgatgata taaaaaacta ataaaaatgg ggggacgact gactgtttta aataacatct
    29521 ttgtcaagca tcatgttccc ccagatattt atgttaagaa aaatttaatg tctcaacgct
    29581 taatgaaagt atttactcaa aatgaaatag agcaagagaa taaagctatg aaaaaataaa
    29641 tatctacaga aaggcaggtg agggaaatag ctaaaaagga aatgtatcat attgatgttg
    29701 tcataagtgc aaaaggcgac ggagaaacaa aaagcaaact aagtgctatg gaaaaataca
    29761 tgaagcagac agaaaaaagg atgcaaacac ttaataggat aaaggttaat cctgctataa
    29821 aagctactga taaagcttca agtgttgtaa atagagttaa taacaatatg aataaagcaa
    29881 aaaaaactgt tacagctaga ataaaagcta cagataatgc gagtccagta gccaatagag
    29941 ctagcaataa tgttaataaa gctaaaaaga cagtaacagc aaggttgaaa gctacagata
    30001 atgcgagttc tactgttaac aaagttaata ataagataaa agaagttgct aagcctatac
    30061 ctcctgtaat catacgagga caagatgaat ctagttctat aatagataaa gtaaaagcta
    30121 agattcagaa tctaaaagct gatactatca taaaaataaa atcacaagct gatgaagcta
    30181 taaacactat ttctcgaact aaaaataaat tacaggaatt tgtgagtaag agatatgaag
    30241 cagcagttaa gattagagat gaagctagtt caactttagg agggttaaca ggaaaaatag
    30301 attcttttgt aagtggagct attagtaaat ttgctagact ggctactacc acaggagctt
    30361 taataggtgg aattggtgta ggttctgctg taaaaggatt tgcaactttt gaacaaagta
    30421 tgaagaatac acaagccgta agtggagcaa caggaaaaga aatggaagct ttaactgcaa
    30481 aagctagaca acttgggaga gaaactagtt ttacagctaa agatgcagga gacgcaatgt
    30541 attatatggg tatggcagga tggaagtccg agcaaatgat aaaagcaatt cctgacgttc
    30601 ttaacttggc agcagcagga ggaacagact tagcactaac gagtgacatt gtgactgatg
    30661 gactaaccgc gttaggaatg actgcaaatg acacaactga atttgttgat gtgatggcag
    30721 caactataac aaattcaaat acaagtgttg agttaatggg tgaaacattt aaatacattg
    30781 gaagtgttgg tggtgcattg ggtgtatcta tgaaagattt atctcttgcg acaggtctaa
    30841 tggctagtgc aagtgtcaag ggaagcatgg caggtacttc gctaagagga ggtctagtta
    30901 gattaataaa gccaccagaa gaagctgcat ctgcaataaa gaagtatgga atagaattaa
    30961 agaaaaataa aaatgggagt ttagatttag caggaactat aggaagttta agagaaaaac
    31021 ttggtggatt agaaaaagtt gaaaaaggag ctgctataca atctattttt ggtcgtacag
    31081 ctatggcagg ttgggcggct gtagtaaatg ctagtgaaag tgattttaat aagttaacta
    31141 cagctattgc agaaagtgaa ggagaagcta agagaattgc tgacatgaaa ctagataccc
    31201 tgtcaggaca atttgaaatt ttaaaaagcg ctattgatga tgtaagaata agtgtaggtc
    31261 aaagactagg acctatgaca agaggatttg tagaagattt aattaagaaa atgccacaaa
    31321 tcggtgatgc tatagttgga gtagtagaaa agtttgttaa taatttcgat aaaataaaag
    31381 caggatttca agtattattg cctgcaattg gttccgttat agcctctgtt atggtactta
    31441 aagccacatt tgcttttgga ggagccatta aaagtttaag tttacttgca agtacttttg
    31501 gtacggctaa attagctgcc ttcggattaa cagttggaat aggggctatt gttatagctt
    31561 ttgcaggaat gactgttgct atttctaata ataaaactgc tatgatggac ttacaaacac
    31621 gttttggttc gtttggtgag tatgttacta caataatgga gactgttggt ggagtcataa
    31681 agcttacgtt aggtaacttg cttataatgt taggtggaat tggaaaagga ataggaatac
    31741 ttttatctga taagagctgg gatgaaaaag cttcatcact taaaaatcta tttggcaaaa
    31801 caacagcaga aattaaaaca aatacaaaag aagctttatc agatataaat ggagaaactt
    31861 ctaatgcaac agctctatta aaaaagtcta cttcaaaaga gttacaaggt gttactaaag
    31921 ctttctcaac agcatttgac caatctaaaa atgtaacaga gaagaaatct agcgatatag
    31981 ctagagcatt aacaaatgga ttaaagggtt tagatgacca atctcttact atgatgagag
    32041 gtttgaatga caatatggcg ataattttat ctggtgtgac agcagatatg aaacctagtg
    32101 acaaagttaa taaaatcacc aaaaatcttg atgatgcttt caaagcaggt aaattaagtg
    32161 cacaagaata cagaagtagc atacaagaga ctttaaattt tataagcaag tatagtgcag
    32221 attcttcaaa taatttaaaa caaggtatga gtgatgcttt caatgccttc aaggaaggga
    32281 caaatatagg cggattaaaa gacggagtaa caggaatgtt gaactcttta aaagcaactg
    32341 gtccacaagc tttagaaacc ttaaaaggtt taggaggaaa agcaagcgag atttttaagg
    32401 gagtggattt taattcttct atagatgcac agaaaactaa agtattacag aatttaaata
    32461 gtttaggatt agaaggaaca caggctatag atactttaag aactattttc tcacaagcat
    32521 catctgcttt ggatactaca aatttaaagc aaggtttaag caatacattt aactctttta
    32581 aagaaggatt taatagtgga ggaataaaaa acgctataaa tagtatgctt ggaactatca
    32641 accaagcagg tccacaaatg caacaagcat taggtaaaat ggatggaaag atggggcaag
    32701 tatttgctaa tgtagacttt agtactccaa tagaaacaca agctagtaaa gttcttgaaa
    32761 atttgaataa tctaggaata gaaggaccaa aagctttaga aactgtaaga agtatatttg
    32821 ctcaagcttc aagtcaaata caaggttcag cttctcaaac ggcacaacaa gcgaatcaag
    32881 aagtggctaa tgctctaact caaggtaatc cagctgtaca acaagcagga caacaattag
    32941 gtacagattt gactaatggt gtagtaaatg gagtacaggc aggagtgcca gttgtacagc
    33001 aaaaaagtaa tgaacttgct acagcaacac aaaacggagt aacaaatgct gtaaatagtg
    33061 caactcctca aatagataat tcaaatctta caagtggtat agatacagca ttcaatcaag
    33121 ctactgcaac agttcagcaa ggggcgagta gtatgtataa tggtgctaaa caaagtttta
    33181 cacaactagc acaaataggt agagaagcag gttcaagtct ttataatggt gctacaactt
    33241 cttttaatat gttagcaaca aatgttagag ttgcatgtag tagcatgtac aatggagcta
    33301 gaacatcttt tacgagttta agcagttcag ctataagtgc catttctgct atgtgtagtt
    33361 ctgtagtaag ccaagttagt gccatgtcga gtcaaattat cagctattgg aatagtgtaa
    33421 gagcaacagt ttcagcacca attacagcat cttttaatgt taagactact caaacaacag
    33481 ttaaaagaac cgtaaatgaa agtagtggtg gaatactggg taatatacta gaccgttttg
    33541 ctgatggtgg agtcgcaaca agaacttcta tctgcggaga gaatggagct gaaatggtta
    33601 tccctcttac aaacactaga agaagtagag caatagaatt atatgaacaa acaggtaaaa
    33661 tgttaggatt aggttcaact ccttctcaaa aagtaggtaa ttctaatatt gtaaataatg
    33721 ttagacaatt tcctcttgca aatgttttag atacagataa taaagaatat aaagaagcta
    33781 caccaaacag tgttaattct tctaataata ctataaattt aggcggaata tctataaatg
    33841 ttcaaaatag tgataacaaa gaagaaatga tacaagaaat attatctcaa gtagaaagtg
    33901 aaataagaga agcattacag gatattggat aatgtcgaat tgttgttaaa aaaatcttcc
    33961 tcataaatga tataatattg ttaaaatatg caaatttatg agggggaaat tttatgtggc
    34021 agaaatttaa aagtctaaat actttcttaa aagttattat aggtattata gcaatatgtt
    34081 tcttaccaat cactttagtt gttttttcaa ttgaattttt aatgaaagct attgaagcta
    34141 aaaataaagg caaaatagtc ttaggatgtt ttttagcatt attaacatta agttgtgcta
    34201 ggactgttta tcttacaaga ggtttagatg ataaaaattc tactcaagtc aatcaagttt
    34261 ctaataataa agataaagaa gattctgaaa agaaaattga agaagaaaca aaaaaacaag
    34321 aggaagttaa gaaaaaagag gaaatagaga aaaattctaa agaggagaat aaaggaaacg
    34381 tggaaacgaa aaaacaaaca ttaactggtg aagaattaaa gaaaaaagtt gattctataa
    34441 ttccagcaga gtataaagga aattattaca caaatgatgt attagataca gatggtagct
    34501 atgtacttag tttacaagtt caaaatgcaa gttttgacaa tgaaagcagt tgtaaggcat
    34561 ttactaaaga cttaattaac aagttgaaag aatttagaat agactcagca gaagtatatt
    34621 ttgtaggttc aagtggtcaa acaacatatc aaattaatat agatgatttc ttgaaagttc
    34681 aagataatat taatagtgta gataatatgg agtttttctc tttcaaagat tttaaaaatt
    34741 agatatataa gaaaaagacg tttatttatt aggaatagat gtctttttta ttgtaaaaag
    34801 gaagtgataa tttggtaata gacatatacc taaaaaatga aaaagaaaaa atagattttc
    34861 attttccagt aaatccattc gatagtttat ctattaaaaa agaaaaaagg tttgaaactg
    34921 tagatatagt aaacttaggc gaatttgaca ttaaaaaaga aggagagaag ataagagaaa
    34981 tatcatttaa aacatttcta cctttccaat atgacgcttc ttattgcaga tacagtgagt
    35041 taaaaaatcc aattgaagta gttgtaatgc ttgaaaaatg ggtagaccaa gctgaaccat
    35101 tacgattaat agtaactggt tttggctaca atggattagt tacaatatct aattttagta
    35161 atactcaaac agcaggaaga gaagaagaca gagatattga gataacattt agaacttaca
    35221 gagaactgaa gatagagaca ttaaagaaag aaactaaaag taatactaaa acagatttaa
    35281 aagacaatag acctaataat aaaaccactc caaaaatata tactgttaag tcaagtgata
    35341 cattatataa gatagctaaa aatcttttag gcaaaggttc gagatggcca gaaatttaca
    35401 atatacccga aaacaaaaaa gtcattggta aaaatccgaa tgtgattaaa aaaggacaaa
    35461 agttggtgat accttctaaa tgaaaataat attaaatgga aaatatgata ttgcaaattt
    35521 taacgaggga ataacattaa gtgaggctat agacggagtt gcatataaga tggatgtatc
    35581 attagtagaa cctaaacaac ttaaagatat aaatattaaa aaaggtgata aaatagttct
    35641 aattgatata gcatatgaga gtaaaaaaga agagacaata tttgatggag ttgtatggga
    35701 aactagaagg agtgaaaaga gtaagaaact gacattgtct tgtagagaaa gaactgttta
    35761 catggaagaa tcagaagaac aatatcagtt caaagaaaat acagcaacac agagaattga
    35821 atattactgc aaacaatgga acatatcata ttacaactta gctaatacag ggaagaaact
    35881 tgctaaagta atacataaga ctaatatact agatatgatt aaaaaagacc taaaagaaac
    35941 agcaagtaag ggtggagact tatttagagt aaggatggat aataagttga aattatttaa
    36001 acttggtact aatgcaaatg tatataaatt agatagtata ttagaagatg ctaactttac
    36061 aagtagtttt aatgatgcag taacaagtgt aaaagtttta ggtaagagta aagacgaaaa
    36121 tacaaaagca cctataattg ggacatataa aaaagatgct gataagtttg gaacactaca
    36181 aaagattaaa caagatgaaa agataaaaaa tgctaaagaa tctaagaaag cagcagaagc
    36241 aatgttcaat agtggagagg aaacaataag tgtagattgt gcagtagata taaatagaat
    36301 aagagcaggt gacaaggtaa gtttaaaaag taaagaatat tatgttatag atgtcactca
    36361 tacactagat tctagaccga aaatgaagct caatataggg actttagaat atataaggag
    36421 gaagttttat acaaatgact gatgctagat ttaatggaat tgctagaata ttgaaagaaa
    36481 atatgaataa aagtgtagca aatggcactt ttggaatggg ttgtgaactt gcagaaataa
    36541 cagcaaatgg acttaaggtt agtggctata aagatgaaat acaggactat ctagtattag
    36601 agaatttaac attaaaagaa gattatttta ctttttcaga tgaagtttca agtggagaat
    36661 atagacataa acataaaata gaaactccaa aggaattgaa gccactacgt ataggtgata
    36721 acgtgctagt agctgtcatg ggggctgaat ttgtagtaat tgggagggtt gtaaatgcca
    36781 aacctatttc ctcaaagtga aacttttgaa actgtagaat taaaaaataa tgatgaaaat
    36841 gaattggacc taaagggttc ttttttattt gattttgaaa aaggtgaatt tgttaaaaac
    36901 gcagatggaa cactaaaaaa atgtgataag gtgcaggcgt acaaacaatg gtgtcaaaag
    36961 gctatattaa cacctaggca caaaaggtca gcttactcta atatatatgg aagtgaagta
    37021 aaagaattaa tcgctagtaa cttatctcaa agtgcaaaag agcttgaaat aactagatta
    37081 ataaaagaaa ctattttggt tcatccttac acaaaagaag taggagagtt tagttttaat
    37141 tggctggaga atagcaggtt agtagagtgt gaatttaatg tattgacaat agatgatgaa
    37201 aatatagtaa ttgatggcaa tataaaaagg taggtgatta tatggaaaga gagctaccca
    37261 taccagtatt tttaacagaa gatgaggact ctgtacatga aaggatgtta agcaactttc
    37321 aagatgtttc tacattagaa ggtgacttta tctatgatgc aacaagacct acagcagagc
    37381 aaatagccga attaaaacaa ctaggattac aaaataattt aaagatagca tttcctcaaa
    37441 ctagctatgg aacttactta gagtggcttg gtgaatgtaa gggagtattt aaaaatcaac
    37501 ctacaaaagc aataggaaca attacattta caggtgtaca aggaactata attacaaaag
    37561 gaactattgt tacaactata gctacagatg aaaaacagag catagaattt gagcttcttg
    37621 aaactaaaac tataggagca aatgaaaaag tagatattaa agcagaatgt aggattgcag
    37681 gaactatagg aaatgtatct aataacacta taactgtttt actaggttct attagtggca
    37741 ttaaatctgt tactaataaa gaagatttaa aaggtgggac agatatagaa gatgaagaac
    37801 attttagaga aagagttctt gtagcagagc aagaagacaa acttagtgga gctagttcag
    37861 attatattcg ttgggctaaa gaagttgatg gagttggata tgcttatgta gttcccgaat
    37921 gggctggagc aggtacagta aaagtattaa tactagataa aaatagaaaa gcagcaacac
    37981 aggaattaat aaataaggtt caagaatata tatatccaaa tgtggaagag ggtcaaaatc
    38041 gagatggaaa agctcctata ggtgctattg taactatagc cactccacaa acattattaa
    38101 ttaatgtaaa agctagtttt gttttttcaa atggatttag tcaagaaact gtgctaaata
    38161 tcttaaaaaa taaaatagac aaatatctgg ataaaataga tattggaggt actgtttctt
    38221 ataatgcaat ttattcacta gcaggttcta tgatgcttgc agatgaagga atacaagatt
    38281 tttctaactt aacaataaat gatggaacaa caaatattat tttaaatgac caagttgttg
    38341 gaataggtga gataatcaat gaggtgatag cgtgataact tctaaaaaag gtagagaaat
    38401 gcttctaaca ttatctccta tctatgaaca atctatcata atgcaaagct tatatgaagc
    38461 tataggaagc gaatttgata atctagagtt attggatgaa gaaatagaat tacaattatt
    38521 ccctcagagc gcaacatggg gacttgaatt ttgggaaaat agggtaggtt tatatactaa
    38581 tatagatgaa gatatagaag ctagaagaag aaaggtcatt gctaagcttc aaatgaaata
    38641 tattgttaac cctgacagat tagcaactat aataaaaagc tatactggtg cagacgtata
    38701 tataaaagaa aatatagctc catacgtttt taaagtaact gctaatgtag atgatgttat
    38761 taattatgaa gattttaaat atataacaaa taaaactaaa ccaagtcatc ttcattggat
    38821 gccttctttt gcactcaaat ttacagatat agaaaaattt gaggtaaaga tgattaatcg
    38881 aatatttata gattttagag ggaatataag taatttctta gatggtatgt ggttattaaa
    38941 tggtagtaaa aacttaagtg cttatatact ttataatgag ccaataagct taaacatgaa
    39001 aaataagtta tttgtaaaag aaagtgaagt atttacaaat cttaaagtaa taattaaaaa
    39061 gaatttatat tacctaaatg ggatagaaat gctaaatgga aataaactgc ttaatgcaga
    39121 attaagagag gaagtgttat aagtggcaaa tgcagtaaca acagacattg caaggcaaaa
    39181 aatgtgtaaa gctagagcag gtgatataac attgcctact atagttagta tggtgtttgg
    39241 agatggaggg attggaaatg atggaaatat aatagctcca ctttcaagtg atatagcgtt
    39301 aaaaaatgag gtgtttagga aagatataga aaattatgta tatccaatcc caactacttg
    39361 cagatattca accactttat taaaaaatga agcagaagga aaaaacataa acgaaatagg
    39421 tttaatagat tctgatggtg acttaatagc aataaaaact tttggaaata agtataaaga
    39481 cagtgatatg gaaatggttt ttcagataga tgatgagttc taggaggtga ataaatgcct
    39541 gatgaattaa attttaataa tgaaatagaa gagtatttaa taactacacc agctcatgcg
    39601 aatgagttta ataatcgaca acaaaaattg ttagacaatg ataaatattt aaataataaa
    39661 attgatacaa ctaaaacaga gttaaatact agaattgaca cagaaaatga gaaacaaaat
    39721 attaaaattg accaattaat tgcaggtggt tcaaatgttg catacactca aagggtcgca
    39781 attgatgatt gggtggagga tgcagaaaat ggattcaaag caactgtaac acatagtttg
    39841 ttaacacaga gaatagttgt aaatattata gatgctacta caaaagaaaa tgtagttaca
    39901 aactttaaaa ttatagatga taattctata gaa
//
LOCUS       DAENCV010000028        39785 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAENCV010000028 DAENCV010000000
VERSION     DAENCV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.028"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            80..1138
                     /locus_tag="KQD82_001392"
     CDS             80..1138
                     /locus_tag="KQD82_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG4379868.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            1389..3533
                     /gene="fdhF"
                     /locus_tag="KQD82_001393"
     CDS             1389..3533
                     /gene="fdhF"
                     /locus_tag="KQD82_001393"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1803..1805,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG4379869.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            3582..4415
                     /gene="fdhD"
                     /locus_tag="KQD82_001394"
     CDS             3582..4415
                     /gene="fdhD"
                     /locus_tag="KQD82_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG4379870.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            4426..4599
                     /locus_tag="KQD82_001395"
     CDS             4426..4599
                     /locus_tag="KQD82_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379871.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDENN"
     gene            4636..5187
                     /locus_tag="KQD82_001396"
     CDS             4636..5187
                     /locus_tag="KQD82_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4379872.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            5212..6588
                     /locus_tag="KQD82_001397"
     CDS             5212..6588
                     /locus_tag="KQD82_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG4379873.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            6588..7103
                     /locus_tag="KQD82_001398"
     CDS             6588..7103
                     /locus_tag="KQD82_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4379874.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(7181..8464)
                     /locus_tag="KQD82_001399"
     CDS             complement(7181..8464)
                     /locus_tag="KQD82_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG4379875.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     KISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            8652..9074
                     /locus_tag="KQD82_001400"
     CDS             8652..9074
                     /locus_tag="KQD82_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG4379876.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            9176..10114
                     /locus_tag="KQD82_001401"
     CDS             9176..10114
                     /locus_tag="KQD82_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG4379877.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            10300..12324
                     /gene="ligA"
                     /locus_tag="KQD82_001402"
     CDS             10300..12324
                     /gene="ligA"
                     /locus_tag="KQD82_001402"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG4379878.1"
                     /translation="MSIKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKASDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            12484..13833
                     /gene="rph"
                     /locus_tag="KQD82_001403"
     CDS             12484..13833
                     /gene="rph"
                     /locus_tag="KQD82_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG4379879.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            13833..14303
                     /locus_tag="KQD82_001404"
     CDS             13833..14303
                     /locus_tag="KQD82_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4379880.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMNIEEDDIKIEEIRI"
     gene            14497..15783
                     /locus_tag="KQD82_001405"
     CDS             14497..15783
                     /locus_tag="KQD82_001405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG4379881.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRANLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            15933..16517
                     /gene="clpP"
                     /locus_tag="KQD82_001406"
     CDS             15933..16517
                     /gene="clpP"
                     /locus_tag="KQD82_001406"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG4379882.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEIFSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            16536..17786
                     /gene="clpX"
                     /locus_tag="KQD82_001407"
     CDS             16536..17786
                     /gene="clpX"
                     /locus_tag="KQD82_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG4379883.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(17857..18384)
                     /locus_tag="KQD82_001408"
     CDS             complement(17857..18384)
                     /locus_tag="KQD82_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG4379884.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(18372..18884)
                     /locus_tag="KQD82_001409"
     CDS             complement(18372..18884)
                     /locus_tag="KQD82_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG4379885.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19064..21427
                     /gene="lon"
                     /locus_tag="KQD82_001410"
     CDS             19064..21427
                     /gene="lon"
                     /locus_tag="KQD82_001410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG4379886.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            21417..22028
                     /locus_tag="KQD82_001411"
     CDS             21417..22028
                     /locus_tag="KQD82_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG4379887.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(22293..23663)
                     /locus_tag="KQD82_001412"
     CDS             complement(22293..23663)
                     /locus_tag="KQD82_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4379888.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(23838..23953)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            24241..25320
                     /locus_tag="KQD82_001413"
     CDS             24241..25320
                     /locus_tag="KQD82_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4379889.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            25711..26391
                     /locus_tag="KQD82_001414"
     CDS             25711..26391
                     /locus_tag="KQD82_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG4379890.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYNNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            26384..27763
                     /locus_tag="KQD82_001415"
     CDS             26384..27763
                     /locus_tag="KQD82_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG4379891.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            27934..29019
                     /locus_tag="KQD82_001416"
     CDS             27934..29019
                     /locus_tag="KQD82_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG4379892.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISIVVTNNLAYIIFSFILFSIGNIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVVDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29073..29432
                     /locus_tag="KQD82_001417"
     CDS             29073..29432
                     /locus_tag="KQD82_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG4379893.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            29485..30357
                     /locus_tag="KQD82_001418"
     CDS             29485..30357
                     /locus_tag="KQD82_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG4379894.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            30358..30801
                     /locus_tag="KQD82_001419"
     CDS             30358..30801
                     /locus_tag="KQD82_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379895.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            30816..31400
                     /locus_tag="KQD82_001420"
     CDS             30816..31400
                     /locus_tag="KQD82_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379896.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            31653..32690
                     /locus_tag="KQD82_001421"
     CDS             31653..32690
                     /locus_tag="KQD82_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG4379897.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            32771..33136
                     /locus_tag="KQD82_001422"
     CDS             32771..33136
                     /locus_tag="KQD82_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379898.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            33141..33458
                     /locus_tag="KQD82_001423"
     CDS             33141..33458
                     /locus_tag="KQD82_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379899.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            33506..35110
                     /locus_tag="KQD82_001424"
     CDS             33506..35110
                     /locus_tag="KQD82_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379900.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            35234..36862
                     /locus_tag="KQD82_001425"
     CDS             35234..36862
                     /locus_tag="KQD82_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG4379901.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            37127..38476
                     /locus_tag="KQD82_001426"
     CDS             37127..38476
                     /locus_tag="KQD82_001426"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG4379902.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            38632..39711
                     /locus_tag="KQD82_001427"
     CDS             38632..39711
                     /locus_tag="KQD82_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG4379903.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16361 a         4250 c         7155 g        12019 t
ORIGIN      
        1 tataaaatat taagattcat agtgtatact aataatatag tatttagtat aaatcttgag
       61 caaaggatgg ttatttctaa tggatagact aaagaatatt agaaagagtt tactacagta
      121 tacattttta tttttgttaa taggtataac aatttaccta gtgtttaaaa cattggatat
      181 taaaatgcta tcaaatgtta tggttatggt agataagaag tttttattca taggtgcttt
      241 ggcaataatg tgtcacataa tgcttgaagg agtagtaatg aagataatta tagaaagcac
      301 tcacaaagtt aatattagat ttattggttt taagctagcc attatgggat tttattataa
      361 tttaataact ccttttgctt caggaagcca accagtacag atatatgtac ttaaaaaatg
      421 taaaatgcca cttagtaagg caagtgcagt tgttaccaat aagtctatta tcttccaaat
      481 agtagttact ttttattgta caatacttgt tttaatgaat tttgctatgt tagaaaaaca
      541 aatgaaaatg attatgcctc tagtgtttct agggatagcc attaattcat ttgtcttaat
      601 tatgataata ttagttattt taaatcctaa aaaaataaaa tattttatga gaattactat
      661 aaagcattta tcaaaaatta agtttttaaa atttttagat aataagatcg aatccataga
      721 aagttttata gatgattata gtaaagcaat aaattttttt gcaaagaata aaaaggtatt
      781 aatttcaact ataatagtaa cttttattca attaagtgct tactttagtg tttcattttg
      841 gatatataaa gcatttaatt tacaaggtta tagctatatt tatatactga cattacaagc
      901 atttttatat atggcaatat ctccaatacc aacacctggg aatgtaggag ctaatgaatt
      961 agcctttttc actatattca aatcagtatt ccctcaacct ttgatgggat atgcagtatt
     1021 tttatatggt ggatttatgt actatttgat tctgattgga agtggaatat ttactgtaat
     1081 tactcactat agaatgaaaa atagaattgg cagaaatatt gttttaagtg aaaactaaat
     1141 aagtttaaag aatatatatt gagtatatta aaaaatatgt cataagaact agaatatttt
     1201 aattaaatgg ttgaaatata aagattatta tgacatattt tttttgaaat ttaagaggaa
     1261 ttgtgagaaa attgttgaat ttaatagata gtattgcaaa atatttatga ttttgcatat
     1321 tataaaaaca ctcattatct ttaagataaa ttttacttat caggggataa atctaagggg
     1381 ggaattgtat ggagaaaaaa attttaacag tttgtccata ctgtggagct ggatgccaat
     1441 tatatctagt agtaaaagat aataagatat taagagctga accggctaat ggtagaacaa
     1501 atcaagaaag tttgtgttta aaaggtcgtt atggatggga ttttctaaat gaccctaaaa
     1561 ttctaacacc taggctaaaa aagccaatga taagaaaaca aggtgtacta gaagaggttg
     1621 aatgggatga ggctataaac tatacagctt caagactaaa tgaaattaaa gaaaaatatg
     1681 gacctgattc tattatggga acaggttctg caagaggacc tggtaatgaa gcaaactata
     1741 taatgcaaaa atttatgaga gctacaatag gaactaataa tgttgaccat tgtgctcgtg
     1801 tctgacatgc accatctgta gccggtctgg cttactcatt aggtagtggt gcaatgtcta
     1861 attctatacc agagatagaa aatgcagatg ttttatttat atttggatat aatggagcag
     1921 attcacatcc aatagtggca aatagaatag taaaagctaa aaaaaatggt gctaaattaa
     1981 ttgttactga ccctagagta actgaatctg caagaattgc tgatatacat ctacctataa
     2041 aaggtggaac aaacatggtt ttagtaaatg cttttggaaa tgtattaata gaagaaggtc
     2101 tttacaataa agaatttgtt caaaatcata cacaaggatt tgatgagtat aaagaaattg
     2161 ttaaaccata tacagctaaa tatgctgaaa agattacagg aatcccagaa gagcttataa
     2221 gaaaagctat gagagaatat gcaaaaggta aaaaagctat gatactttat ggaatgggtg
     2281 tctgtcaatt tggtcaagct gttgatgtag ttaagggact tgcatcaatt gctctactta
     2341 caggtaattt tggtagagaa agcgttggaa taggacctgt acgtggacaa aataatgttc
     2401 aaggagcttg tgatatggga gctcttccta atgtatatcc tggctatcaa aatgtaactg
     2461 atgataagat aagagaaaag tttgaaaaag cttggggggt aaaattatct cctaataatg
     2521 gttatagttt aactcaagtt ccagacttag tactaaaaga gaaaaagctt aaagcttatt
     2581 atatatttgg agaagaccca gtgcaaagtg acccagatgc atcagaagtt agagaagcat
     2641 tagatgaatt ggaatttgtc attgttcaag atatatttat gaataagaca gcacttcatg
     2701 ctgatgttat acttccagct acttcttggg gagaacatga gggagtatat acatgtgcag
     2761 atagaggatt ccaattgatg agaaaagcta tagagccaca aggagatgta aaaccagact
     2821 ggcaaattat aagcgaaatt tctacagcaa tgggatatcc tatgaactat aaaaatacta
     2881 aggaaatatg ggacgaactt agacaattat gtccaagttt cttaggtgct acttatgaaa
     2941 agatagaaac tcaaggatgc gttcaatggc cttgtaagag tgaaagtatg gaagataaag
     3001 gaactatgta tctgtatgaa ggtcaaaagt tttctactcc taatggtaaa ggtaatttgt
     3061 tcgctgctga atggagaccc cctatggaag ttgaagatga tgaataccca tttagtttat
     3121 gtactgtaag agaagttgga cactattcag taagaacaat gactggtaat tgtagaacac
     3181 ttagttcatt agaagatgaa ccaggacgtg ttcaaataaa ttctaatgat gctgaaaaat
     3241 taggaattga agatgatgaa ttagttagaa ttagttcaag aagaggaagt gtaataacaa
     3301 gagcaacagt tactgataga gttaaggaag gtgcaacata tatgacttat cagtggtggg
     3361 ttggagcttg caatgaactt acaatagcaa atttagaccc tatatctaaa acaccagagt
     3421 ataaatattg tgctgttaaa cttgaaaaac ttgaagacca agaattagca gaaaaatgtg
     3481 taagagaaga gtatcaaagt ttgaaagaca aaatgacagc tacaaatatc taatgattta
     3541 ttagatttaa gatttataaa aggcagaaag gctaatattt tatgaacaat atatactcaa
     3601 aaactaaatt acaagaaaat agtaaggata aagtttttga aaatagttat agtctagtgg
     3661 actacaaata tttaaattct tttaatgtag aagtaagcaa aataaatgaa catgaagtat
     3721 caatagataa tgaagaaata attgcagaat atccacttag ttttatttta aatgataaat
     3781 atataaatac atttttatgt accccatata atttagagga acttatagta ggattcttga
     3841 aaacaaaagg atacatagaa aacaaaaaaa gcttattaaa atttgagata aatgagagtg
     3901 aacgttttgc aaaagtaact attcaagaga aacaaaaaaa tcaagatgag caaattatat
     3961 ttttaaattc actagactat ataacatgtg ctcctgtaaa gagcaatata aaaataaatt
     4021 atgatacgat ttacagtata atggataaaa atttaaattc atcagaatta tttaaaaata
     4081 ctggtggggt acatagtgta tctgtattta attttgataa aaaaatagtc acttgtgaag
     4141 atgtagcaag gcataatgca atggataagg ctattggatt ttgtgtttta aatgacataa
     4201 cattaaaaga taaaattgtg gctgtaagtg gaagaatatc attggaaatg atattaaaag
     4261 cagctatgac tcaaattcct attgtagtat caaaatcagc accgactaat ttatcaatag
     4321 agctttctaa aaaattaaat ataacgctta ttggatttgt aagaggaaga cggatgaata
     4381 tttatgcaaa tccacagaga gttatcaaaa agtgagaatg aatatatgtg tactaaaaaa
     4441 gaagagcaaa gatattcaaa gtgtgaaatc tgtggagtat taattgatga tactgataag
     4501 gtatataata acacatacaa tttgtgcaaa aaatgtaaac gaaatataag tactgagaaa
     4561 atagtgaaat atataaatat atatgatgaa aataactaga gggttggaaa tatacaaatt
     4621 gaaaggtggg ataatatgaa aaataaaata ggctcttttg taatagcaga cccaaacaaa
     4681 tgtacaggtt gtagagcttg tgaagtagct tgttttacaa tgcataatca gcataataat
     4741 gtgggttata cagttggaac agttgatata cctgttatac ctagacttta cttagtaaaa
     4801 ggtgacaatt tctgtatgcc aatacagtgt agacattgtg aagatgctcc ttgtttaaac
     4861 tcatgtccac aaaaagctat agttaaagaa aacaatatta tgtctgtaaa tgaagaaaag
     4921 tgtataggat gtaaaacttg tctgctagca tgtccttttg gagctataga cttacttcca
     4981 caatatgaag atggtaaaga agtagaacaa gtaattttag atgaaaataa gaagatagct
     5041 tataaatgtg acttatgtaa agacaatgaa aaaatagctt gtataagtgc atgtcctcag
     5101 gaagcattaa aacttgttac tccaatagat gacaaaaaag ctaagaatag acaagctgca
     5161 cttagtttat tgttaacaaa taaataaagg atacaggggt aggtgacgtt tatgataata
     5221 aatatagata aggatttatg tactggatgt agagaatgtt caaaggtctg tccagtaaat
     5281 gctattgaag gtgaagaggg aaagccacaa gagataaatt tagatagatg tgttatgtgc
     5341 ggtcaatgtg ttcaaacttg taaatcttat gcctcagtta tagatgaagg atttgagttt
     5401 ttacaagaga aaaaacaaga aagagagata ccagagtcta taaatgaacc tatatttgca
     5461 gcatacaatg tatgtgatat agataaggtg aagaaagctt taagtgaccc aaatgtattc
     5521 actatggtcc aatgtgcacc agcagttaga gttgctttag gagaagattt cggatattat
     5581 ttaggatact taggtgctgg gaaaatggca gcagcactta gagctttaaa ttttgataga
     5641 gtctacgata ctaattttgc agcagattta actataatgg aagaaggtag tgaattaatt
     5701 aaaagagtga cagaaggtgg aactcttcca atgtttacat cttgttgtcc agcttgggta
     5761 aaattcatgg aaaaaaatta tcctaaatta actaatcatt tatcttcttg taaatcacct
     5821 cagcagatgg gtggtgcaat atttaaaact tatggtgcag agataaataa tgtagatgca
     5881 tcaaagatat ttagtgtttc tataatgcca tgtacatgta aaaaatatga atgtgataga
     5941 gaagaaatgg attctagtgg atatagagat gtagatgtag tattaactac tagagaacta
     6001 gcttatttaa taaaagacat gggaattgat tttaaaaatt taagagaaga aaaatttgat
     6061 agtcctttag gtagttatag tggagctgga actatctttg gtgttactgg tggggttatg
     6121 gaagctgcta taagaacagg atatgagctt ataactggag aaagtattcc tgatgtagaa
     6181 gtaaaacaag taagaggaga aaatggtttt agaaagtcaa ctattaaaat aggagactta
     6241 gacttaagag ttggagtagt aagtggatta aaaaatgtaa taccagtgct tgaagacttg
     6301 gaaaaaggaa aattagatgt agattttatt gaagttatga cttgtcctgt tgggtgtgta
     6361 agtggtggag gtcaaccaaa aattttacta gaagaatata aggaattggc ttatgaaaat
     6421 agaacaaaag caacatacgt gcatgatgaa catttagcaa ttagaaaatc tcatgagaat
     6481 cctgatatta aaaaactgta tgatgaatat ctggtagaac cattaggaga aaaatctcat
     6541 catctattac acactacata ttgtatagga aaggaagtgg ctaagtgatg aactattttg
     6601 ttatagcaga ccctgataaa tgcataggat gtagaacttg tatgatagga tgtgtagttg
     6661 cacattcaga tgaagatatt ttttaccaaa atccagatga aataaatttt aatccaaaac
     6721 tttctgtcat aaagacaaaa gaagtaagtg ctccaataca gtgtagacat tgtgaagatg
     6781 ctccttgtgc aaaggcatgt ccaaatggtg gtatagttag ggttggtaac acaataaaaa
     6841 tcaatgaaga aaattgtata ggatgtaaga cttgtatgtt agcatgtcct ataggtgcaa
     6901 tagacatagt tacattaaaa gatgtagatg aaggaaagct ttgctttaga gaaagaatga
     6961 cagcaaataa gtgtgatttt tgtatagaat cgccagaagg gcctgcatgt gtaaatgttt
     7021 gtccaactaa ggcatttact atagttaagg atgaggatat agatagtaaa gttaaaaata
     7081 agagaaaatt agctatattg taatataaaa aagatggagt tttatttatg attaaaagct
     7141 ccatcttttt tattataata tttatatatc taaaaatcaa ttattgcatt agtttatcta
     7201 atctttcttt attcaatttt ttcattccta tagaagttat aataagtaaa gtataggcaa
     7261 ttataactga taaaattata atagaccaag aaaatctgta tccaaaatta tcaactaaca
     7321 atccaaataa tgttgaacct aatgcaaatc ccactgcaaa aattaattgt acgattccat
     7381 atatgctacc aaactccttt tgaccaaaga aagagccagt taaataagca ggaccaatca
     7441 tataagcaaa tacagaaaga cctttcaaaa tggcaaatac aaatgcaaaa gtagaagatt
     7501 tatctaccaa tagtaatgat aaagttgcta taagaaccaa tacaaaagat ataataagtg
     7561 gctttgtttg accgaattta tcaaacaata atcctccaaa taaattacca atgagagcaa
     7621 ataaagcaaa tgtggagcct actgttccta ttagaatagg atttagataa tttctaagat
     7681 aagttggata ttgtacagat aatgatgaaa catatatacc aacaaatata aatccaatag
     7741 tcataatcca aaaatattta atttttttag cttcttttaa tgtataaccc catactgaag
     7801 gagaaatttt atcagatgct ttctcttttt tacctttaga ttttaccttt tcattaatat
     7861 ctttaggcat cctgagtata aataatatta caggtatacc aactattaat gctataattc
     7921 caaatattag ataagaacga ctgtatcctt gagttgctaa taagcgaact gcaagtggtt
     7981 gtagaaatat actaccaata gaaccaccag caaaagcaaa gcccaaagct tttcctttgc
     8041 tttcttcatc aaaccaagca cttattaaaa caggtattcc tatagcagat ataattgtag
     8101 cgccaatttg aacaatacat gatacagtgt aaaattgcca tagttcagta catatagaaa
     8161 aagtagcaaa agcaatacta gaaattatac aacctaataa atatagtatc ttaatattga
     8221 ctttagaaaa taatacacct ataaaaggtg atgcaattgc tgaggcaata gtaccaatag
     8281 tgaatattag agaaaaagaa gctaaactaa agccttctcc ttctacaaca taacttataa
     8341 attgaggctg tatacttgct acaatactaa atggaacagc ttgtattaac atacaaccaa
     8401 gtactataac ccaagcattt gaaaagtttt taatgctaaa tttattgttt agtaatgtat
     8461 tcatggtatc tctcctttag gttatgattt gtagatatta atctaattat tctaatttta
     8521 tatattagtt ttggatagcc taactattta aatataattt gggtattaat ataaaaatta
     8581 actataaaaa ttgattattt taaatgtatg gggtaaatta aagtatataa ataaatgaaa
     8641 ggatggataa aatgtcattt gaaaaaataa ataataataa cctagataaa atacacaaca
     8701 ggaagtgtat tatgcttgta aactttaata aaaaagaatt atcattaatt aaaaacgtaa
     8761 gtaactttgc tggaataaga gaccatatca tcttaagtga taaaaatgga gagaatataa
     8821 taagagacat tttagatgga aatatatcaa atgaatgtga aaatgggatt aagagtaagt
     8881 ctgtaatatt taataacact ccaagcacac aagtaaatgc atttttagaa gggctaaaaa
     8941 aagtgagaat aaatagacct tttacagcta tggtaacaga gacatctatt gattggacat
     9001 tgaataattt aatagaaaat ttagttcaag aacgtaatgc tatgtcttta ggaaaaacaa
     9061 ttgaacataa gtaaatttaa gatatcttat agtaatttta actaatgtac attgagtatc
     9121 atattatatt ttagaatata aaattttaaa tgtgggtaga tggaggagat ttgagatgaa
     9181 agttttattt acaataaatt atggaaaaga aaaatttgat aaaataagag aattaggcta
     9241 tgatgtcata cattactctg aagatagtat tgaaaataat gaggatgtaa atacagcaga
     9301 tgtactagtt acatacaatc catttgagag attagatatt tctaagatga aaaatttaca
     9361 atatatacag acaactagtg taggtataga ccaacttcca aaagaagagg tattgaagag
     9421 aaatattaag atagctaata atagaggcgg atatagtgta cctattggtg aaagtattgt
     9481 attgtatatt ttagaaatat ataaaaatag tgcaaaattt tttaaacaac aacaaaataa
     9541 agaatggaag atggattttt ctgtatcaga attgtatgga caaagagttg gatttttagg
     9601 aacaggtact atatcttcag aagcagcaaa aagattaaaa gcttttggtg tagaaatatg
     9661 gggtgtcaat actgatggaa gtagtaaaaa atactttgat aagtgttttg ctactgagga
     9721 tatgaatatt gtatttgaag aatgtgatat agtagttgta gcaatgcctt ctacaaaaag
     9781 tacagatgga atagtaaaca aagatatgtt taaacttatg aaagatggtt ctgtatttat
     9841 aaatgtaggt agaggaaata cagttaatca gaaagattta gaagaatgtg tagagaaatt
     9901 tagaggggta gcattagacg tatttgaaag tgaaccttta aacaaggaca ataagttgtg
     9961 ggagtgcgaa aatgtcatag taactcctca taattcttgg gtgtcagata aaaacaaaga
    10021 gagaacattt aatatggttt ataataattt gaaacactat ataatggaga ataaacctgt
    10081 agataatgtt gtagacatat caagaggcta ttaaaaataa aaaattttga taaaaataaa
    10141 aactcttatt agtcgtatta aagatactaa taagagtttt ttttgtaata aatttaaagc
    10201 caaaaaagtg atatactaaa aaatatataa ataggagata attaattata gggaaatttc
    10261 aaaataattt aattttaaaa atatgaagag gtgcaaaaag tgagcataaa agatgaaatt
    10321 aataaactta tagagacaat aaactaccat aatgaaaggt attataatca agatagccca
    10381 gaaatatcag attatgaata cgataagtta atgaaggagt taatttcttt agaagaagaa
    10441 catccagaac taaagagaat tgattctcca acaaataggg taggaggtaa gccattagat
    10501 aagttcaatc aaattgtaca taaaactcct atgttgagtt tatcaaatgc tttttcagag
    10561 caagatttga gagattttga taagagagtt caagaatatg tgggagaaaa tgttgaatat
    10621 gttgttgaat ttaaaataga tggtctttct gtaggtctaa cttataacaa tggagagttt
    10681 gaaaaaggag caactagagg agatggagtt gtaggagaag atatttctca aaacttaatg
    10741 acagtaaaaa gcatacctct taagataaat gataaaaaag aactaatagt tagaggagaa
    10801 gtatatatat caaaagataa ttttagaaaa gtaaatgaac aacaagaaga acaagaacag
    10861 ccattatttg ctaatccaag gaatcttgca gctggttcat tgagacaatt agacccaaaa
    10921 ctaacagcaa agagaccatt agatatattt gtatttaata tggaaaatat agaagaatac
    10981 gatttagaaa gtcacagtga atcactagag tacttagaaa aactaggatt ttcagtaagt
    11041 cagagttata aagtttgtaa aagtatagat gaagttattg agcatattga atactggaca
    11101 gataacaggg gtaatttaga ctacgaaata gatggtatgg taataaaagt aaacaatata
    11161 aaacaaagag acgaaatggg atatacagcc aaaagtccta gatgggcaat tgcatataaa
    11221 ttcccagcag aaaagaagaa aactaaaata gtagatataa tagtagaagt tgggagaact
    11281 ggtacaataa caccttcagc aattcttgaa ccagtcagac ttgcaggtac tactgtaagt
    11341 agagctacac ttcacaatga agattatata agagaaaaag atataaaaat aaatgataca
    11401 gtattagttc aaaaagcagg ggacataata cctcaagttt tagaagttat aaaagaagaa
    11461 cgtactggag atgaaataga ttttaaaatg ccagaaaagt gtccagtttg ttcagagcca
    11521 acaataagat tagaaggaga ggctgctgta aaatgtatca atatgtcttg cccagcccaa
    11581 ataagaaggg gaattataca ctttgtgtca agggatgcaa tgaatataga tggattggga
    11641 gaatctataa ttacactgct attaaatgaa aaaattattc aagatgtatc tgatttatat
    11701 tatataaaaa aggaagatgt agtcgaccta gagagaatgg gcgaaaaatc tgctgacaat
    11761 ttaataaatt caattgaaaa atcaaaagaa aatgatttat ggagacttat aaatggatta
    11821 ggtattaaat ttataggagt taaagcagct aagatacttg catataactt taaggattta
    11881 gatgaagtaa tttctgcaac tagtgagcaa ctagaagaat tagaggaatt tggaagtata
    11941 atggcaaaca gcgttgtcga gttttttaag gaagacaaaa atatgaacgt tataaacaag
    12001 ttaaaaaatg taggagtaaa tacaaaatca ttggataata caggtgaaaa agtagaaaaa
    12061 atatttgaag ggatgaaaat cgtattgact ggtacacttc ctacattaaa aagaaatgat
    12121 gcaaaagata tgatagaaaa aagaggtgga aaagctacat ctagtgttag taaatcgaca
    12181 acatttgttt tggcaggtga agaagctggt tctaaactta ctaaagcaag tgatttagga
    12241 attaaagtta tagatgaaga gagattttta gaattattaa agttatcatc gaaagatgaa
    12301 gtaaaagctg tattagagag ttaaaaagtc gcttaattta gtagaaaact actataatta
    12361 atgatagaat agttcagtaa gctaataaga ttacgaaaaa tgacaataat taatatttga
    12421 caaaattaaa aattataaaa ataaatttat aaaaaataaa aaatatgaga aaggatataa
    12481 aaaatgacca gaagttatga tagaagtaat gaacagataa gacctgtaaa gataactaga
    12541 gattatacaa agtatgcaga aggttctgtt cttatagaaa tgggagagac aaaggttata
    12601 tgcactgcat cagtggaaga aaaagttcct ccttttttaa gaaatagtgg tactggatgg
    12661 ataaatgctg aatattctat gctaccaaga gcaacacaac aaagaaagat aagagatgca
    12721 tctaggggaa aaattgatgg aagaacacaa gaaatacaga ggttaatagg aagagcaata
    12781 aggtctgttg tagacttgaa taaattagga gaaagaacta tttgggtaga ttgtgatgtg
    12841 attcaggcag atggtggaac tagaactgca tctataacag gggcatttgt agcagttgca
    12901 gaagcaatat ataaattata taaagcaaag acaataaaag agatgccaat aacaaatttt
    12961 gtatctgcaa ttagtgttgg tattgtaaat ggaatacaca tgctagattt atgttatgaa
    13021 gaagattcta aagcacaggt agatatgaat ataataatga ctgataagtg tgagtttgta
    13081 gaagtacagg ggacaggaga agagaaacct ttttcgagag cagatttaaa tttattgtta
    13141 gagttaggtg aaaaagggaa taaagaatta ataaggcttc aaagagaagc tttaggtgaa
    13201 atagcagatg agatattggg tatggattat ggtaatgaag tagtgattgc tactaataat
    13261 gctcataagc tagaagaaat aggtgaaatc ttaaaagatt ttgaatataa agtgtactct
    13321 ttaaaagatg ttgatttagc aggtatagag atagtagaag atggaaaaac atttgagcat
    13381 aatgcgttga taaaagctag aacaatagca aaaaagacta aacttatagc aatatcagat
    13441 gattctggct tagaagtaga tgctttaggc aaaaaacctg gtgtttattc agctagatat
    13501 gcaggagaac atgcaacaga tgaagaaaat agaaaaaaac tgttaaaagc tatgcaaaat
    13561 gtaccaatga gcaaaagaaa tgcaagattc gtctctgcaa tagcagtagt ttttcctgat
    13621 ggaaaagaat ttgtagtaag aggaatttgt gaaggaatga taggatttga agaaaaaggt
    13681 aaaaatggtt ttggatatga tagcttattt atagtaaaag gatatgataa gacttttggt
    13741 gaaataccta gtgttataaa aaattcaatc agtcatagag caaacgcact aaaacttatg
    13801 aaacaagagt ttataaaaag ggtagtgaag taatgaaaat aggcatagta agcgacacac
    13861 atatgatgat taaaaatatg gaaaaaacaa taccatattt gaaagaatgt gacctaataa
    13921 ttcatgcagg agataacttt actgattcaa gatatattca tagcatgact aatgtaggta
    13981 ttattgcagt taaaggtaat tgtgactttg atgctgttga ggaagaagtt gtctttgaag
    14041 tggcaaataa aaccatattc ttatgccatg gagataagta tggtgtaaaa tatggaacta
    14101 atatgttaga aaaaaaggca actgaagttg atgctgatat agttatattt ggccataccc
    14161 ataccccatt tagagaaatt aaagatggtg tactttacat aaatcctgga agcacttcac
    14221 ttccaagagg agtatcgtat aaaagttttg tcattatgaa tatagaagaa gatgacataa
    14281 aaattgaaga aatacgcata taaaggtgta tttatctaaa aaaatggcaa aaaacgctca
    14341 aaaaattaaa ttttatcaaa taagtacaac gatattaatg gaacacttgt aaataaaaaa
    14401 acttatgtga tataattaag tggttgaatt ataaatagaa tgaattataa taccgttatc
    14461 gggtattaca taaatatcac taggaggatt agaaggatga aagcagaact aattaaaaaa
    14521 gagggtaata aagttacttt aaaaataaca gtagataaca ataaatttga agcagcagta
    14581 aataaggctt ataataaaag cagaaataaa tacaatatac ctggatttag aaaaggtaaa
    14641 gctccaagaa ttgttataga gactcaatac ggaaaaggta tattctacaa tgatgctata
    14701 gaaatattat tcccagaagt ttatccagaa gctatcaagg aattagacat agacccaata
    14761 gacaatccag atttagatat agaagaaata agtaaagaca atgggttggt tatggttctt
    14821 aatgtagaag ttaaaccaga gtttgaatta ggaaactaca agggtataga aatagctaag
    14881 gttgagaata ctgtaagtga cgaaaatgtt gatgctaaac tacaagaaat ggttgaaaaa
    14941 aatgccagat tagtaagtgt agaagacaaa gctttagaag atggagatac agctataata
    15001 gacttcgaag gttttgaaaa tggtgtagct tttgatggtg gtaaaggtga aaattataac
    15061 ttagttatag gttcaaacac atttatacca ggatttgaag aacaattagt aggtaaaaaa
    15121 gctggtgaag aagtagaagt taatgttact ttcccagaag aatatcactc acaagattta
    15181 gctggaaaac cagttgtatt taatgtaaaa ataaatgatg taaaagtaaa agaattatct
    15241 gcacttgatg atgaatttgc taaagatact agtgaatttg attctttaga tgaattaaaa
    15301 gcagatgtaa gagctaattt agaagaagaa gctaaaaata gagcagatgc agaaactaga
    15361 aattcagtag tagaaaaagt tgctgaaaat acagaaatag aaataccaga agttatgata
    15421 caacatcaaa tagataatat gttaaatgaa cttaactacc aattacaata tcaaggattt
    15481 ggattacaac aattactaga gatgactggt agaactatgg aagaattaag agaagagaga
    15541 aaagaagatg ctaaaaaatt agttaagtct tctttagtat tagaagcaat aactaaagct
    15601 gaaggaattg aagcaacaga agaagaattt aaggctgagt tagaaaaaat ggcttctgct
    15661 tacaatatgg aagtagaaaa aatagaagct tctttaagag atgctgacaa agaagatata
    15721 aaaggtcaaa taaaaataag aaaaacaata gatttattag ttgataatgc aacaatagct
    15781 taataaaata aacgctaact aatatagttg acgttatgaa atgtttagta tataatgatg
    15841 tgcaaatata atttagttaa atgcgtataa taatttacag tagcttaaaa aaagctactg
    15901 taaattatat agaataaagg gggtataaaa ccatggcatt agtacctgta gttgttgaac
    15961 aaacaggaag aggagaaaga tcttatgata ttttttctag actattaaaa gacagaataa
    16021 tcttcttagg agatcaagtt aacgatgcca cagcagggct tatagtagca cagctattat
    16081 ttttagaagc tgaagaccca gacaaagata tacatttata tataaattct ccaggaggaa
    16141 gtataacttc tggaatggct atatatgata ctatgcaata catcaaacca gatgtaagca
    16201 ctatatgtat aggtatggct gcttctatgg gagcattctt gttggcagca ggagcaaaag
    16261 gaaaaagact agcactacca aatagtgaaa taatgataca tcaaccatta ggtggagctc
    16321 aaggtcaagc aacagatata gaaattcatg caaagagaat tttaaaaata aaagagactc
    16381 taaatgagat attctctgaa agaactggtc agccattaga aaaaattaaa atggatactg
    16441 aacgtgataa cttcatgagt gcactagaag ccaaagaata tggtttaata gatgaagtat
    16501 ttacaaagag accgtagata gagagaggtg ttaatatgtc taaatatgaa gaaaaacgac
    16561 aattaaagtg ttcattctgc ggtaaaaatc aagatcaagt aagaagatta attgcaggtc
    16621 caaatgtata tatttgtgac gagtgtgttg aactttgtga cgaaattatt caggaagaaa
    16681 tagaagatac tatagatgaa gatactacat ctttaccaaa accaaaggaa atgatggaga
    16741 ttctaaatga ttatgtaata ggtcaagaaa aagctaaaaa agctttatca gttgcggttt
    16801 ataatcatta taagagaata tacagtaaaa aatctagtag taaggatata gaaattcaaa
    16861 agagtaatat acttttatta ggacctacag gttctggaaa gacattactt gctcaaactc
    16921 tagcaagaac tctaaatgta ccttttgcaa tggcagatgc tacatcattg actgaagctg
    16981 gatatgttgg agaagacgtt gaaaatatac ttttaaaact tattcaagct gctgattttg
    17041 atatagaaaa agctgaaaga ggtataatat atatagatga gatagataag attgcaagaa
    17101 aatctgaaaa cccatctata actagagatg ttagtggtga aggtgttcaa caggcacttc
    17161 taaaaatatt agaaggtact gttgcaaatg ttccaccaca aggaggaaga aaacatcctc
    17221 atcaagaatt tttaaagata gatactacta atgtattatt tatattaggt ggagcttttg
    17281 atggattaga gaagataatt caaaaacgtg gtggagataa aacactaggt tttggagcta
    17341 aaatagaaag taaaaaagaa ttagatttag ggaagctata tgaaaaagta cttccagagg
    17401 atttattaaa atatggtata ataccagagt ttataggaag aataccagtt ttagctactt
    17461 tagagttgtt ggatgaagat gcattaatgc agatattaca ggaacctaaa aatgctctag
    17521 taaaacagta taaaaagtta ttggagttag atgatgttga gctagaattt gaagaaggtg
    17581 ctcttcgtgc aatcgctaaa aaggccatag aaagaaatac aggagctaga ggacttagaa
    17641 gtatagttga aagcgtaatg atggaaacta tgtttgaagt accatctaga gataatataa
    17701 agaaggttat agtaacagag aaatctgtga acgaagactc agtaaatcct atcatagtac
    17761 ttaaggatca agaggaaagt gcataagatg atgaaaagga gctttaaaag ctcctttttt
    17821 tatgttaata tgctaaatat aaaataggta tcattattat aaaaatgtga gtaatatacc
    17881 tagaatacca aagacgaata caattgcaat ggaaccaatt tttcctttat ataaaagata
    17941 aaatgatgct ataaatgcaa ttattccata taaatcccaa ttaacttctt tggcagatga
    18001 aaaagctgct gataatatga atcctagagt aattggtcta agaactgtca ataaattttc
    18061 taccttttta ttatttttaa atttagaaaa taactttgat ataatagtta gacctattaa
    18121 tgcaaaaatt gtaacaccaa tagttgcagc tagagaacca aagaagccag cagtcttaaa
    18181 tcctacaaat gtagcactat ttattgctac aggacctggt gtagattgtg atatagcaag
    18241 taaatccaaa aactcttgat tagttataaa gtgatattta gatataaatt cttgctggat
    18301 aaatggtagc atggcataac cgccaccaaa actaaatgcc cctattttaa ggaatattaa
    18361 gaagagtagt attatttctg acatagacta tatacacctc ctccaattat taaataaata
    18421 gggcttatat tgaatacacc aacagataaa actgctataa ttaatagtag tatgttagtt
    18481 ttagactttg gtagtttctt gaacatgctc gtaaaagaat acaatataag agctattaca
    18541 acaggcgcaa ctcctttaaa aaatccatct aaaattttag atgatgaatt tttaaagtat
    18601 agatacgata agactaagac tatacaaaat gatggtaaag cagagcctaa ggtacatata
    18661 agaactcctg gtataccata aagtttatat cctaataata cagatatatt aactgccagt
    18721 actccaggat aactttgagc tatggataaa taatcaataa attcttcctg ctctaaaagt
    18781 ttttgtttgt ttacaagttc atcttgtata agaggaacca tagcgtatcc accaccaaat
    18841 gtaaacgcac caatttttaa aaaggttaaa aacaatttca acataataga aacctccaaa
    18901 agataaataa catctaagta tactaatatt aatattatat cataataaaa agataatata
    18961 gtatgatagt attctaagaa tttatcatta atgttgaaaa ttaacttgtt caaatatata
    19021 atattaaaag caaagcaatt agactagaaa aggagtaatg ttgatggaac aaaattatac
    19081 taaaatagat catgaattac cattaattcc tctaagagga ttagctatat ttccatatat
    19141 gatattaaat tttgatatag gtagagagat atctcttaaa gctttagacc aagctatgat
    19201 ggatgaagaa cttatatttt taacttctca gaaagaagca gaggtagatg agccaggaga
    19261 agaggatttt tatcatgtgg gaacaatctg taaagttaag caaatgataa aattaccagg
    19321 agatacagtt cgtgttttgg ttgaaggtgt atcaaggggt agagtaaaga aaatagaaca
    19381 agaagatggg tatttcagag cagtaataga agaaatagta tttgatagtg ataatttgga
    19441 tagtgaaaca gaagttgaaa ttgaagcatt tgtaagaaat gtatttgatg cctttgaaga
    19501 gtatataaat attggaaata gggtatcacc agaaatatta atatctttag ctgatataga
    19561 agatgtagat agatttatag atacgatagc tgcaaacata tatctaaaat caagccaaaa
    19621 acaagaaata ttagaagaat ttgatattag aaaaagactt gaacttattt attctatatt
    19681 attagaggaa atagatatat taaaaataga aaagaaaata actttaagag taaaaaaaca
    19741 gatgaataag gttcaaaaag aatattatct tagagaacaa ttaaaagcta tacagaaaga
    19801 attaggtgaa gaagaagata taaactctga agcagatgag tatagagaaa agcttaaaaa
    19861 aataaaagca cctaagacaa ctaaagagaa aatagaaaaa gagatagata aattttcaaa
    19921 aatatcttct atgtctccag atgtatctgt aagtagaaac tatttagata ctatattctc
    19981 attgccatgg aataaagaga caaaagataa attagatata actaaggcaa aagatatatt
    20041 agatgaagac cattatggat tagaaaaagt taaggagaga atactagagt atctagctat
    20101 aagaactctt gctaagtcac ttaaaggtcc tataatatgt ctagttggtc ctccaggaac
    20161 aggaaagaca tctatagtaa aatcaatcgc cagagcatta aatagaaaat ttgtaagaat
    20221 atctttgggt ggagttagag atgaagcaga aataagaggt catagaagaa cttatgtagg
    20281 ttcaattcca ggaagaataa ttaatggggt aaaagaagcc caaacaaaaa atcctgtatt
    20341 cttatttgat gagatagata agatggcagc agattataaa ggtgacccag cttcagcaat
    20401 gctagaagta ttagacccag agcaaaataa ggattttgtg gaccattact tagaaatacc
    20461 ttttgattta tcaaaaatac tatttgttac aacagcaaat agtctaggta atataccaag
    20521 accattatta gatagaatgg aagtaataga agtatctggg tatatagaag aagaaaaatt
    20581 aaatatagca aaaaaatacc tacttccaaa acagattaaa gaacatgctt taaaagaaag
    20641 ctttataaaa atagatgatg aaactttaag aagtataatc aaccattata caagagaagc
    20701 tggagtaaga acactagaaa gaactattgg taagatttgt agaaaggttg caaaaaaata
    20761 cgttgaagac ccaactcttg aagaggttgt tataaataag tctgacctag agacatatct
    20821 tggaaaagac atgtttaagt atcaacttgc agaagttaac cctcaaatag gattagtaaa
    20881 tggtcttgca tggacagaag ttggtggagt tacacttgaa gtagaagtaa acgtacttaa
    20941 aggtaaagga gaaattgtat taactggaaa attaggagat gttatgaagg aatctgcgaa
    21001 gacaggtatc tcttatataa ggtcaatagt ggacaagttt gatatagacc ctgaatttta
    21061 taagacgaat gatattcata tacacattcc agaaggtgct gttccaaaag atggaccatc
    21121 agctggtata actatggctc ttgcagtaat ctcagcactt actaaaagac ctgtgcctgg
    21181 aaatatagca atgacaggag agataacttt aagaggtaga gtcttagcag taggaggagt
    21241 taaagaaaag ttattagcag ctcatagagc tggtataaca aaagtattaa ttcctaaaga
    21301 atgtgaagct gatttagatg aaataccaga aaatgtaaaa gaaaaaatgg agtttgtgct
    21361 tgtagaacat atggatgaag tattagaaca agctttatta aagagtggtg aaaataatga
    21421 aaattagaag ttcagagata actatgagtg ctgtgaataa atcacagtat ccagcagaag
    21481 gaatcccaga gatagcattg gctggtaggt caaatgttgg aaaatcatca ataataaata
    21541 cattgcttaa cagaagaaac tttgctagaa ccagtcaaac accaggaaaa actagaacta
    21601 taaattttta tctaataaat aatgaatttt attttgtaga ccttccaggc tatggatatg
    21661 caaagatagc taagtctgaa aaagaaaagt ggggaggtat aatggaacga tatctagaaa
    21721 gtagacaaga gttatgttct atattcctac ttgtagatat tagacatgag cctacagcag
    21781 atgataagct tatgtatgaa tggataaagc actttggata taactgtgta gttattgcaa
    21841 ctaaggcaga taaaatatct agaggacaat atcaaaaaca cataagtata ataagaaaaa
    21901 aattgcaaat ggaaagtagt gaaaaagtaa ttcctgtttc ttcactaaaa aagactggtg
    21961 ttgaagaatt atgggaagag attgtaaatc aatacaatca acatggatat gaaataacag
    22021 tagattaaat ttaaaatgtt aaatattgac ataagatttg tttgaataat aaatgtaata
    22081 atagttagtt ttaattatat taatattaaa gcaaaatata ggatgcctta aaatagtttt
    22141 aatgaacttt ttttggcatc cttattgttt ataaacaata aatatcagta attttgctaa
    22201 atactaataa attttagtaa ggtatgggaa aattagtata aattagttca actaaaaaat
    22261 tattgactta tgtagtcagc aataaatagc atttaagaaa tagatttatc tgtttttctc
    22321 ataaagaaaa cagttagtat aagccctata aaacatactg atgctgcaat ccaataagac
    22381 tgattgctag ctataacata ggctgatgtg tgacttttaa acttttgatt gtatgtaaga
    22441 ctttgtgcaa aaaaactact aaaaaatgct gttccaagag caaatgataa attacgaata
    22501 gttgcaagaa aactgcctcc gtatccctgt ttttgaggag gaacatctgc taaaatacta
    22561 ctattgtttg gtgaactaaa tgctcctagc ccaagtccaa aaataactaa acatacaatt
    22621 aaaagataca atggagaatc tgcttttaaa agtgccattc caactagtga gatggtcata
    22681 aatgaaaaag atgctgttag aatgtttttt gcaccgtgtt tatcagaaag tgaacctgca
    22741 ataggtgcta taagtaccat aataagtgga tgtacagtca ttacataacc agccatcata
    22801 ggagataaat tttttaattg ttctaaataa aatggaagta gaaaagatac tgccatttga
    22861 ggaaaataac aagctactcc aatgatattg cctacagtaa agtttctatt tttaaataga
    22921 ggtaggtcaa taaagctttg ttcaaatttt acttctctta atgccaacaa taagaagatt
    22981 ataagtgctg ttatacctaa atacaatctt gtattaccct tctcattcat tgcaagtaat
    23041 ataagcgtag tgcaagatgc gaaaagtaga agacctggaa tatcaaagct cttgttttgt
    23101 tcatccaaaa caggtttagg cagaaattta atgccaagaa agactgctat aataccaata
    23161 ggtatattga taataaaaat agaaggccat ccaaaatgtt ctaaaattat acctccaatt
    23221 acaggaccac tcatatttcc tataccaact acagctccag tgattcctat agcttttcct
    23281 ttttcatttg ctggaaatgc attggaaact ataccaagcc cagtagccat taaaatactt
    23341 gctccaactg cctgaaagat tcttgaagaa attaatgttg ataaattgtt tgacatacta
    23401 cacagaaaag aacctattgt aaatataaaa aatcctaatg tgtatagacg atttgaacct
    23461 actttatcgc caagttttcc gaaaaataac atagtagctg ttacaacaag catataagct
    23521 gttacaaccc actgaatttg tgacatgtta attttaaagt cagcagcaat tgtaggattt
    23581 gcgatattta gtatacttcc atcaagagta gacataaata caccaataca gatgataatc
    23641 aatactatcc atttttgttt catgataaca ctccaatctt aatagatttt ataagtacga
    23701 tataattgta aaataaacaa caaaaaaagc acctgtatat taggtacctt gaatatgtct
    23761 aatttcatga ataaatacgg tagttgaagg atttgtatag attactcatg taaaattaca
    23821 taaaggtact catatactat tttcttctac catccagatt ttactgtcgg ctttggaata
    23881 tgaccaaatt atgccataag gctcgcgggc ttttactgcc gatagggaat ttcacccgtt
    23941 tatgaagaaa aacagatgta ttttgtaata ttattttaac ataaaattat aatggaaaca
    24001 attggattta agtaatatcc agctgtatta caatacaaat atttcaatat ggacacttaa
    24061 attgtattta tagatagaat aaggaacagt atgagaatct ataataatta gataaaatta
    24121 acctgttatt agtattagat ggcaagtttt aaatttatca aaatcagaaa gctgtaataa
    24181 aattcttata atatgaatat cttaattaag agatattgta aattatgagg aggcgctaat
    24241 atggaaggaa aggtaaagaa ggcattcccg ggtggaaata catcacaagg ctcatattct
    24301 ttttttgact atataattcc tgaaaatgta aaaagagttt tttgtttaaa gggtggacct
    24361 ggagtaggaa aatcctcact tatgaaaaaa atttataaag agtttttgca tagaggatat
    24421 gatattgatt tatatcattg tccttctgac ccaagttcgt tggatggttt agttataaag
    24481 aaattaggcg tggtacttat ggatgcaact gccccacata ctatggaccc aaagctacct
    24541 ggagctttgg atgagataat taatttaggt gattactgga atacagaaga attagaaaag
    24601 aataaacaag aaatatcagt ttatgataaa gatattagta attcatttaa aagagcattt
    24661 aagtttttaa aatcagcaga accaatcttt agagacatag aagaaaaata ttctgattgt
    24721 atggattatg gaaaagtaaa tttggtcaca gaagaattta taaaaaggtt atttgatgga
    24781 gttaaaagca caggaaatct aaaaagagaa aagcatttat ttggttcagc tataactcct
    24841 gtagggtgtt tagattatac agaaaacatt ttaaaagatg taaagaaagt ctattattta
    24901 gatggagaaa taggaactgg gaaaactaca ttattaaaca aagtatataa gaaagctatt
    24961 gaaaaaggtt taaatgtaga agtttatcat tatccactta ttccagagaa aattgaaact
    25021 gtactattaa ctgatttaga tataggcata actataagta cgacttttaa agaagaagat
    25081 acaattgatt taaatcagtt tttaaataga gaaaaactac ttaaatatga agaagatatt
    25141 aaatttgatg aaaaagtttt agatgattta atttgttggg cagtactaaa tcttaagaga
    25201 gccaagtcta aacatgatat tatagaaagt tactatagtc ctaatatgag atttgatgaa
    25261 gtagggaaat taagagataa gttaattaaa agaattttaa aatatgagga aaacttataa
    25321 acgtatatgt ttaaaattaa tgttaataat aatataattt ttagtaaatt ggtgtagctt
    25381 tgaggctatg ccattttact ttttttgcta aaaaatgata ttaataatct ccaaagtcat
    25441 tatttgtttt tcataattat tatttccgtt cctaatttat gtttttttat aatatcacaa
    25501 caatgtttat tgaaagttta tataatttta agaacatcta aaaatgcata cttgatattg
    25561 gtaaattagt gataggagtt caatgtatga tattatatac taaagatgaa ttttaatata
    25621 taactttgat ataataagca tatgtaaatg tttaatattt aattaaagaa aattatcata
    25681 taaaaataaa tttgtaagga gcaatacagt atgaaaaaac aatatgtgtt tataatacta
    25741 gcaagtacta ttttaggtat acttgttgga accatattta gagattataa caatgaagaa
    25801 acagtttata caagcaaaga caagtcaata agaaaagaaa taaaaatgac aagaaaatct
    25861 ataaaagaat taaccaaaga gaaagacaag ttagataaag aaattaattc aatgaaacaa
    25921 aatcaaaaaa attctgaaga aatatcagaa atagaaacac ttagagatag tctatcatat
    25981 tcagatgtaa agggtagtgg gataatatta agtatagatg ctatagatga aaaaacaggg
    26041 aatatagcta atagtataga ctataataag attcttgtaa aagttataaa tgaattaaaa
    26101 gtaaacggag gaaaatacat agaaataaat ggtcaaagaa ttaatcaata ttcagaagtt
    26161 gtacttgcag gaagccatat aaatataaat ggagttcctg tagcacaacc atatgaaata
    26221 aagtgcattg gagataagga gaaactatct aattatgtag ataaaggtag taaatattta
    26281 gatagcatac aaaccaatta tccaatgaaa ataagcataa aaactgaaga aaatatctct
    26341 atgaaaaaaa tgaatatacc taataaatta aagtatataa agggtgatta agattatatt
    26401 agaggaaagc agtttgccaa tgttaatacc agaaaaactt gcaaaaaaat ataatgtatt
    26461 tccagttgag tttcaagatg ataatttagt tgtagaaata gaagatgagg atatttatgc
    26521 tctacaggac ttgagattag ctacaggtaa agaaattata ttgaagaagg agaaaaaaga
    26581 acttataagt gaaaagattg aaaaatatta taatagcttt gaacttgatg aggattatgc
    26641 aaaaaaatta tttgaaaatc ttttagaaaa agctataaaa gaaaacgcaa gtgatataca
    26701 tatagaacca tttaaagaac atttaatagt gagaatgagg gtagatggag aactaaagga
    26761 agtatcaaaa tttttagttg atgtctatcc atcattgtct acggttataa aattaaaggc
    26821 atcaatggat ataacagaaa aaaggcttcc tcaggatggt agggtggata taaagttagg
    26881 tggaaattta attgacatta gagtttcatc aataccaact gtctatggtg aaaaaatagt
    26941 tttgaggata cttaatagag atactttttt aaaagacaaa ttagaacttg gtttttcaga
    27001 ggaagctata aaatctataa atcgtataat aaataaaaga gcaggaatat tattagtgac
    27061 aggtcctaca ggtagtggaa agactacaac agtatattcc ttattaaatg atttaaaagg
    27121 aataaataaa aatattatga ctatagaaaa tcctgtagag tataagatgg atggcataag
    27181 tcagattcaa gtaaattcaa aagtagggtt gagctttgat gtaggtctta gagcgatatt
    27241 aagacaagac ccagatataa taatggtcgg agaaataaga gatgcagaaa cagctaagat
    27301 agcagtaaga gcagctataa caggtcattt agttataagt actttacata ctaatgatgc
    27361 agtatcttcc atagctagat tactagaaat gcaaatacca ccatatcttt taaatgcctc
    27421 tcttataggt gttatatctc aaaagttggt tagaaaagtc tgcaatcatt gtagccatga
    27481 gattatgata aaagataatt ttagtggaga tgtaaatact aaggtagctg tagggtgtga
    27541 aaaatgtaat gataaaggat actttggaag aacagcaatt tatgaaatct tagaaattaa
    27601 tgatgatata aagacatgta taagaaatat ggaggattct agtactatta aagatgtggc
    27661 acaaaataat ggaatgataa catttgaaga tagttgtaaa agactaataa atgaaaaaat
    27721 aacgacatta gaagagtgtt tagtagtaaa tgaaatgatt taactgaatt gtatagttaa
    27781 acttcgtttg gtcagttgtc tttatttaat ctattttaat taagatgttt gagtataaat
    27841 tatatttaag tgaaataaaa ttaaatgcat taaattagaa aatattaagt ttatttaatt
    27901 gtaattaata taatttggaa ggtattatag tacatgagat tatctaaaaa tatattttat
    27961 aatttagaaa agttttattt agtattaaat agaatcagtg ataaagaaat tagagtaatt
    28021 tgcaaagaga taggcatatt gctggaatct ggatgtgaaa ttacaaaaat atttgaaatc
    28081 attgagagtc agtcaagtaa aaaagtaaag aatttgctta gtatagtatc aaatcatatt
    28141 caaaaaggta actcaatagc agaatcattt caaataacag gtatattttc taaattcttt
    28201 ataagtatga taaaagcagg agaaactagt ggaaacttag atataataat gagtgattta
    28261 tctaattatt atgataaaga atacaaatta aaaatgaaaa taataactat ttcaatatat
    28321 ccaataatac ttataatatt atctatctta tcaatgttgt ttatattcgt atttgtaata
    28381 cctaattttc aggtggtttt taccaacaat ggaattgaac cacctttaat aactagagtt
    28441 ttaatgggaa tatctatagt ggttacaaat aacttagctt atataatatt tagttttata
    28501 ttgttttcaa taggaaacat ttatttcttt ataacaaatg ataatataaa aaaattgatt
    28561 aattcgttaa aattcaaaat accttttata aaaaaaatta atcagttagt agctacaaca
    28621 agattttcaa gggcattgtc cgttttaatt agcagtggaa tacaaattgt ggaagctata
    28681 gacatgtcag ctagtgttgt agacaacgaa tttatttatg aaaggttaaa gatttccaat
    28741 aattatataa aaaaaggaaa tagcataggt aaatctttga atttagcaaa tatttttcca
    28801 aaattattta tttcaatggc aaatacagga gaagaaagtg ggaaactaga taaaagtcta
    28861 aatgtaataa ataaatttta tgaaaatgaa ttaaataaca aaatagagca aataatgaaa
    28921 tttatagaac ctatgctaat tgtatttatg ggattaatta ttggtacttt tattttagca
    28981 atggttgttc ctatgtttga tgcaattact aatttttaga gagaaaaata aattttatta
    29041 gatgaaactt ttgaaaagga gagaatattt aattgataaa tttgataaat aaaaaacgaa
    29101 aaggatttac acttgttgaa atgattgtag tagtaactat tttaggcgtt atatctagta
    29161 tagcattagt taagtatagt aaggttcaag aaagtgccaa attaaatgca gactatacga
    29221 atgctgctaa tatagtaacc gcagctagca tggcaattaa tgatgatgaa aagacaatag
    29281 actctctaag tgtagagaca ttgaaggaaa agggatacct aaatactgtt ccagttcctc
    29341 agagtacatc aggtaaattc gaacttgtca taaatgatag cggaacagat ataagcgtaa
    29401 atataaattc taagcaattt tatccaaaat aatattttat attgattgct gtaagtttta
    29461 aaattatttt tatttaagaa ggagatggct ataattaaaa aaattctaaa cttagaaata
    29521 aatgatgatt atataaggct tgtattttta agaaaaagtc ataaaaaagt ttttatagat
    29581 aaatcaatta taaaagagat taactttgat ataaaaaatg atagtaattc gattgagaag
    29641 attaaatata tagaagatgt agttagattt gtaaaagagg agattggtaa atcaaaaata
    29701 ttatataaaa atttatactt taacattcaa attcaggata tagtcattag aaatgttgaa
    29761 gttctaaata ctaagaaaaa aagagacata ttaagcatga ttgaatttga aataacacaa
    29821 tacattccaa taaacataaa taactattca ataaaatata aagttataaa ttctaaaaat
    29881 gataaattga atatacaagt aattcttttc ccaaagagta ttattcaatt aattaagaac
    29941 atttcagaaa atctaaatat gaatccaaag actataaata taaattttga tatattacaa
    30001 aagcttataa acttaaattt aatagagaat ttttcagaag aaggggtatt tatagaatgt
    30061 aaaaaaagtc agttaattat aaatataaca aaagataaaa aaataagaga aacatacttg
    30121 ctatcaaaag agggaaaatc atatgagttc ataaaaagat tattaaggga ttttaagtat
    30181 gtttattact atggtataga aaattgtttt attgaagaat actttaaata tacagataaa
    30241 tctattgaag taaatccatt aagattaaaa gataaagtaa aagtagaaaa taattttgat
    30301 gcagatttag aagacaatag gataaattat ataaatagta taggaatgat tatttaagtg
    30361 aaagtggata aaacaataaa ttttcttaat ggatttaaaa aaactaaacc atgtaataaa
    30421 atctataaaa taataatagt cttaatttta acgtgtattt tatttaaatc atttatgtat
    30481 gtgaaatcta taaatgaact tgaagattat ataagtcaaa ataagctagt ggataaatca
    30541 aagaacactt taccaacaac agacaaaact agagagaaat tttctaaaat aaaacttaat
    30601 gaaataaaaa aaatatatga aacaattgga ataaatagta ttactaaaat atcaatagat
    30661 aaaaataagc tagagatgga aggtgtgtgt agtgatttag atgttttaaa caatttaagg
    30721 aaaataaagt acataaaata ttattacata gacagtattg taagagatgg agaatattat
    30781 agatttagaa taaaaagtta gatgggtgat tatttttgag attaaataat agagaaaaaa
    30841 atctacttaa aatattgtta atattagtta ttatattggc aagtgatata gcagtaagtt
    30901 atttggaaga aaacctaact agtttgaaga atgaaaagca aaatataata aataaaataa
    30961 ataatcaaga taattatcaa ataaaggata ataagcatat agatgataaa tatatagaaa
    31021 gtgttgaatc aaataaaaat gaagaattaa aaaataattt taataactca tttttagata
    31081 aaaaaacaaa taaagatata gtgttattag tagaaaatga actgagagat attgtgaatg
    31141 tagaatatat taatcaagaa agtagtttag ataatcacaa taaaaaacaa gttaatttag
    31201 aaataaaagt taatggagaa cttgcaaatg tgttgaaact tgaaggtgta atgaagaagt
    31261 taagactaaa taataaactt gaaaagatag aaattaataa attaaaagtc aataattttg
    31321 atgttcaaaa tattccaaat gaatataaac aagaaaaaga caatattgtt gaatgtaaaa
    31381 tgacagttag agtcatataa caaaaaagaa tagataaagt aaaaaactat tatcaattaa
    31441 taaaatttta tagaggaatt agttttacga taattcattt ctacaagagg gattgttgat
    31501 gattttatcg aaatcctaat taaagttaat atattgtacc aaaatataag tgaatttaat
    31561 agaatattta cttatactat agattgatta ataaaaaaat ggtataataa caaattgtac
    31621 tttaatttaa taataaggga gtggaagtca tattgttagg gagaaatgag ctatgccctt
    31681 gtggaagtgg caaaaaatac aagaaatgtt gtttaaataa agatattgtt tcagaaagag
    31741 catgcagaaa aactgaactt tcacaaaaac aatatacaga tttatattca aagttatatg
    31801 attattcaag acaggataag tttaaagaag aatgtgaaaa agctaaagaa atgttttata
    31861 taatgcaaaa tgatagtgta aatgaaaaat ttgaaagatt ttttaatact tatttaatac
    31921 aagaccacat aatggaaaat aaaaaggtta tgacagttgg ttttcttgaa gaaaatggtg
    31981 caaacctaag tcaatcagaa gtatcaatat taaaaagtat gtttgaatca tacgtaagta
    32041 tttatgaggt aaaagaagtt tctgctggaa aaatagtatt aaaggactgt ttaagtggaa
    32101 atgaactttg tacagaagat gtaaaacttc tgagaagttt taaaatcgga agttgtatga
    32161 tagctagaat agttgatatt gaaggaacta acatattgat agatataact ataagtatat
    32221 caaatgaggt taaagatata attttaaagg atattatgaa tctattcaat caatatcaag
    32281 atgtatacaa agatatgaag tcgttcttaa tttatcatac acatatacta tacaagtata
    32341 tacaacagct tttagaccca agtatagctg aatatttaaa gagagaaaga gataaaaacg
    32401 atacaaaagt taattcagaa aatgaattag ctgaagaaga atgtaaagtt ctagatactc
    32461 ttaaagaaaa tgtagaaaaa gaaaatatag atgcatgtat agagttttgg aatgaatata
    32521 agaaaaatca tgaaaatata aaaggagctg aaaatggttg ggcagctgct gttgaatact
    32581 atgttaaaaa agaagcagga caacctataa ctcaagttca aatatcaaag aaatacgata
    32641 taagctctag tacactagga aagagataca aggatttaaa aatatcttaa ttaattataa
    32701 aaagctttaa gtcatagata aagaatctat agtataatat atgtataatg tttagaaaga
    32761 ggtattaata atgagtctta gtttaggaaa tgcaattgct gtatcaacga actttataaa
    32821 atctaaccca gctgtacctg tgtacaacgg agaagaaata tatgcagtta tttatcatat
    32881 agaaatagga tataataatg gtgtatcacc tgataccaaa caatatataa taatagacta
    32941 taaagagttt gatttatcta tggataaaaa aaatgaacta ataaatattg ctaaagagta
    33001 ctgtaaatct aaaggtattg tagaaaacga aaaccttgaa ataaaccttt tagacaatga
    33061 agaagcagag aacttcttag aaaaggtgtt taataatatg aaggttataa gaggtcttat
    33121 aactaaaaaa ggataaataa atggactata taggaataga aaatataaca ccttatgaaa
    33181 atacatatga atttagtgta tatgaatatg atgatgaaat caccttaggt agtgaaaagt
    33241 tatatgtatg tgaattaagg gttgtattga ttaaagttaa ttctctgtat gttgaaagat
    33301 tgcataaatc agttgaagca atggtcttag taaaaaactt gaaaaaagat ttagataaaa
    33361 cacttgttgt aaacaaaata aagaattttg tgctagatga gatttgggta gaaaatctag
    33421 taaaagagaa tatagaagtt atatttgtag aaagctagga ataaccctag cttttttata
    33481 ttacaatcta agagaggaaa acaatatgtt aagagtatct aatgtaaaac ttggaattga
    33541 taaagatata tctttattaa aagatgttat acttaagaag ctaaaaataa aagaaaaaaa
    33601 cttagtcaaa tatagtatat ataaagaatc tatagatgct agaaaaaaag gaaaaatgga
    33661 atttgtttat tctgtagatg tagaagttaa agatgaaaat aaaatactta actcaaaaat
    33721 aaaagatgta acaaaaataa aggaaatcag atacgtaaat gtacctgtgg gtgataaaaa
    33781 attaaagaat aatcctttgg taattggtag tggaccagct ggcttgtttg caggtctgct
    33841 tttagctcaa atgggttatg aaccaatcat acttgaaaga ggtcttgatg tagacagtag
    33901 aaccaaagat ataagtgatt tttggacaac tagaaaatta aaaaataatt caaacgttca
    33961 atttggagaa ggtggagcag gaactttttc ggatggtaaa cttacaacta gaataaaaga
    34021 tatacgatgt agaaaagtat tagaagagtt agttcagttt ggagcaccag atgaaatatt
    34081 atactcacat aagccacatg ttggaacaga tatattaaaa aatgtagtta aaaatataag
    34141 aaatgaaata ataagtttag gtggtcaagt tagatttaat tcaaaagtaa cagatatagt
    34201 tgtatcaaat gattctatac aatctgtaat aataaatgat actgaaaaaa tagatacaga
    34261 tacaattata ttagctgttg gacatagtgc aagagatact tatgaaatgt tgtattcaag
    34321 aggtataaaa atagttcaaa aaccttttgc aataggagct agagttgaac atccacaaga
    34381 tttaataaat aaagcacagt acaaggattt ttataatcat cctaggttag gagctgcaga
    34441 ttatagactt atagagcatg tttcaaatgg aagaacagca tatacttttt gtatgtgtcc
    34501 aggaggtagt gtcatagcat ctgcatcaaa tgattttgaa atagttacaa atggtatgag
    34561 tgaacatgca agagataaaa taaatgcaaa tagtgcattt ttagtaaatg taacgccaga
    34621 agattttaag agtgataatc cactagcagg tgttcatttc cagcagaagt atgaaaaatt
    34681 agcttttgaa ttaggtggaa aaaattataa tgcaccagtt cagcttgttg gagattttat
    34741 aaataataag acttctttga atttaggaag tgttgagcca tcatatagac caggatatac
    34801 acttacaaat ttaagtgaat gtttgcctag cttcgtaaca gagactatga gagaagcgtt
    34861 tatatctctt gataaaaaat tgaatgggtt tgcaatgtat gatgctgttt tgacgggagt
    34921 agaaactagg tcatcatcac caataagaat agtaagagat gaaaatactt tagaatcagt
    34981 gagtattaag aatctatatc catgtggtga aggtgctgga tatgcaggtg gtatagttac
    35041 agcagcagtt gatggtataa aatgtgcaga aaaaataata caaaaataca ccaataatac
    35101 aaaaatttaa cataaactta atgatttttt aatatttaac atgaatttaa cattatcact
    35161 cctataattt gataaaatac aataggttat aaaattttta gatacagtaa tctctttaca
    35221 aggaggaaat aatttgaaca ttataataag tctaatgggt ggattaggtc tgttcttata
    35281 tggtatgaac cttatgggag aaggtcttca aaaatctgct gggacaaagc taaaaaagat
    35341 aataaaacta cttactagta acctatttat gggagttttg gtaggtacag gagttacagc
    35401 agtaatacaa agttcaagtg ctactactgt aatggttgtt ggtttcgtaa atgcaggaat
    35461 aatgacttta aaacaggcaa taggagttat aatgggtgct aatataggga ctactgttac
    35521 agcccaattg gtatctttcg acttaactgg tatggcccca gtagcattag gtgtaggtat
    35581 tatactttat ttatttggaa ataagcctag aataaaaaat atagccgaaa tacttatagg
    35641 ttttggtata ttatttacag gtatggattt tatgaagatg gcagtggagc cactaagaga
    35701 ctatcaagga tttactgact tacttgttac atttggtaga tatccactac ttggattatt
    35761 attgggattt gggattacag caataataca aagttcaagt gcatctatgg gtatgttggt
    35821 tgcactggca gcagaaggtc tagtgccact atcagcagca ctgccaattc tttatggcca
    35881 aaatattggt acatgtgtaa cttcactgtt atcaagtatt ggtgctaaca aaaatgcacg
    35941 aagagcagct atgatgcatc taatcttcaa tgttttagga acagtgatat tcttaatatt
    36001 cttgaataaa ccagtagtaa gcatggttac aagttgggac ccaagcaatg ttgcaagaca
    36061 aatagctaat acacatacat tgtttaatat aatatctgta ttgatacttt taccatttac
    36121 aaatcttatt ataaaactag ctataaagct tgttcctgat agagcaggtg atatagatga
    36181 agatgaaact aaaactatta aatatataga tgatagaatg atagaaactc cttctatagc
    36241 tcttgcaaat acgataaaag aagcacttcg tatgggtgag aaggcgaagg aaagcctaaa
    36301 tgcatctatg gaagctttag tagaacattc aactgaaaaa atagataaga catataggcg
    36361 agagagatta atcaatgatt tacagaaagc tattttaaat tatttactta aactttcaaa
    36421 agcaccttta gatgatgatt caagagaagt tgtggatact ttgtttaata cagtcaatga
    36481 tatagaaaga attggtgacc acgctaaaaa tatagctgaa ttatcgcaag ttgcaataga
    36541 ttcaaatatt tctttctcag aagaaggaca atctgaactt gatgttatgt ataatagagt
    36601 tgtttcagca tatacttatg cattagaatc aatgagaact gataatgttg atttagcttg
    36661 taaggttata aaaattgaag aacaagttga tataatggag aaatcttgta gagctaatca
    36721 catgtataga ttgaacaata atctatgtag tatagaaaat ggagttattt atttagacgt
    36781 aataagtaat ttagagagaa tatctgacca tgctgtcaat atagcacaac aagttatagc
    36841 aaagagatta ggaaatgatt aaaaataatt aatttaaaat aatataaaat ttaaaataga
    36901 ggataaataa aagagaaact ttaaatgata cgtttagagt ttcttttttt gtaaatttat
    36961 ttgactaaaa tttttaaatt tatacatagt tatagcattt agaagaatcg atttcctgat
    37021 aataaatgat gagatatgta tgattttgtg gcaaactttt aaaaaaaaca cctatatttt
    37081 gtgttataat ctctttaaaa cgaatatgct gaaaagaggg atttaaatgg gaaaaataaa
    37141 ttttgattat agtaaagcta cagagttttt ttgtcaaaac gaaatagatg tcatgcaacc
    37201 atatgtagat gttgcgcacg acatgttaca taataaaact ggtttaggaa atactttttt
    37261 aggatggata gaccttccta aaaattatga taaagaagag tttgacagaa taaaaaaatc
    37321 tgctgataag ataaaatcag actcagatgt tcttttggta ataggtatag gaggctctta
    37381 tttaggttcg agagctgcta tagatatggt aagtcattct tttaggaatg gacttaaaaa
    37441 agagcaaaga aaggctccag aagtatattt tgtagggcat aatataagct caacatatat
    37501 tatggattta ttagatataa ttgaaggaaa ggatatttct gtaaatgtaa tatctaaatc
    37561 aggaactact acagaaccag ctctggcttt tagaatattt aaagattact tagagaaaaa
    37621 gtatggtaag gaagaagcaa gaaagagaat ttatgcaaca acagatgcaa gtaaaggtgc
    37681 acttagacag ttagctacag aagaaggata tgagactttt gtaataccag atgatgtagg
    37741 tggtcgtttt tctgtattga cagcagttgg gttacttcct attgctgctg ctggacttga
    37801 tattgatgct atgatgaaag gcgcaaatga tgctagagaa gcatttcaaa atcctgactt
    37861 aaaaagtaat gatagttata gatatgctgt tgctagaaca attttacata gaaaaggaaa
    37921 agatgtagag ttattagtga actatgaacc tcaactacat tatgtaagtg agtggtggaa
    37981 acagctttat ggagaaagtg aaggaaaaga aaataaaggt ttattccctg cttctgtaga
    38041 tttctcaaca gatttacatt ctatgggtca atacatacaa gatggaaaaa ggcttttatt
    38101 tgagacagta cttaatgttg aaaactgtaa gagaaatata actataagtt cagaggaagt
    38161 agacttagat ggtttaaatt atttagcagg caagactgta gattttgtaa atcataaagc
    38221 ttttgaagga actctattgg ctcatacaga tggaaaagta cctaatttag taataaatat
    38281 acctcaacta gatgagtata attttggata tttagtttat ttctttgaga aggcttgtgg
    38341 aataagtgga tatttattgg gagttaatcc atttgaccaa ccaggagtag aagcttacaa
    38401 gaaaaatatg tttgctttac taggcaaacc aggttatgaa aaagaaaagg aagagctaga
    38461 aaaaagactt aaataatttt aaatagttaa ggtttaatta agattaaaat aatataatga
    38521 aatcatcgaa aaggagttta ctcaaaatag atgatttcat tttttttatg aaaaattggg
    38581 aaaaatatat tttgataatt tatattatat taagataggg agtggtatga gatgtcaaga
    38641 agaaagaaag ggataagttt agtaatatta gttgctataa taagttcaat acttagtagt
    38701 tttctaacaa taattttagt taaggacaat ttagtaagta aatctacagg aagttctacg
    38761 ccgatagttg taaatgatga tggtaagagt caaaatattt atcaagcagt tgcagaaaaa
    38821 gctacgccat ctgttgtagg tattacaaca actagtgttg atactagtaa tatgtttgca
    38881 ataccaactg aaacacaagg tgtaggaact ggaatcatag ttgattcaaa tggatatata
    38941 cttactaatt ctcatgttat atcagatggt caagctacaa gtgtaaatgt attattcaat
    39001 gatggttcaa caacttcagg taaagtagta tggtttgacc aacaacttga tttagctata
    39061 gttaaggttg ataaaactgg actaacacca gcagaatttg ctgatagtga caaagtaaaa
    39121 gttggagata tatcaatagc aataggtaat cctcttggat tagattttca aaaaacagtt
    39181 actcaaggga taataagtgg attagataga actatacaaa cagaaaaaac taatatgact
    39241 ggtttattac aaactgatgc aagtataaat gcaggaaata gtggaggacc attgttaaat
    39301 caaaaaggtc aagtaattgg tataaacaca gcaaaagctt ctcaagcaga aggattagga
    39361 tttgcaatac caatcaatac agctaagagt atagttgaag aagtaatcaa aaatggaaaa
    39421 tatgaaaaag taactttagg cataaaaggt acagatgtat caaattatga agcagcaaca
    39481 ggtacaaaat taagtactga taaaggtgtt tatgttgcag aagtaatatc tggttcatca
    39541 gcagaaaaag ctggagtaaa ggtaggggat attataacta aagtaggaga tacagatata
    39601 acaggtatga atgatttaaa taaaaagttg tatactttct caaaaggtgc tagtacaaaa
    39661 ataacagtaa atagaggcgg aaaagcagta acaataaatg tgaatttcta aatatgacca
    39721 gaaagataga gactacatgg taaagtgtag tctctatttt tatttaattt ttatagtata
    39781 tacat
//
LOCUS       DAENCV010000029        39539 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAENCV010000029 DAENCV010000000
VERSION     DAENCV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.029"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            32..1144
                     /locus_tag="KQD82_001428"
     CDS             32..1144
                     /locus_tag="KQD82_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG4379904.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KQD82_001429"
     CDS             1398..3422
                     /locus_tag="KQD82_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4379905.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KQD82_001430"
     CDS             3642..4328
                     /locus_tag="KQD82_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG4379906.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KQD82_001431"
     CDS             4423..5478
                     /locus_tag="KQD82_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG4379907.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="KQD82_001432"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="KQD82_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG4379908.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="KQD82_001433"
     CDS             7195..8901
                     /locus_tag="KQD82_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG4379909.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFKEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDSVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="KQD82_001434"
     CDS             8923..9996
                     /locus_tag="KQD82_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG4379910.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="KQD82_001435"
     CDS             10008..10730
                     /locus_tag="KQD82_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG4379911.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDLNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="KQD82_001436"
     CDS             10746..11903
                     /locus_tag="KQD82_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG4379912.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGKVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="KQD82_001437"
     CDS             11906..12745
                     /locus_tag="KQD82_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379913.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="KQD82_001438"
     CDS             12760..13935
                     /locus_tag="KQD82_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG4379914.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="KQD82_001439"
     CDS             13964..14713
                     /locus_tag="KQD82_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379915.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="KQD82_001440"
     CDS             14735..15643
                     /locus_tag="KQD82_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379916.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="KQD82_001441"
     CDS             15691..16443
                     /locus_tag="KQD82_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG4379917.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="KQD82_001442"
     CDS             16397..17764
                     /locus_tag="KQD82_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG4379918.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="KQD82_001443"
     CDS             17770..18861
                     /locus_tag="KQD82_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG4379919.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="KQD82_001444"
     CDS             18945..20858
                     /locus_tag="KQD82_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG4379920.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="KQD82_001445"
     CDS             21057..21764
                     /locus_tag="KQD82_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG4379921.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="KQD82_001446"
     CDS             22066..24177
                     /locus_tag="KQD82_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG4379922.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="KQD82_001447"
     CDS             complement(24375..25340)
                     /locus_tag="KQD82_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG4379923.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="KQD82_001448"
     CDS             complement(25625..26581)
                     /locus_tag="KQD82_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG4379924.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="KQD82_001449"
     CDS             26949..27737
                     /locus_tag="KQD82_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4379925.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="KQD82_001450"
     CDS             complement(27860..28330)
                     /locus_tag="KQD82_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG4379926.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="KQD82_001451"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="KQD82_001451"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG4379927.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="KQD82_001452"
     CDS             29156..30061
                     /locus_tag="KQD82_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4379928.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="KQD82_001453"
     CDS             30376..31923
                     /locus_tag="KQD82_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4379929.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="KQD82_001454"
     CDS             31926..32324
                     /locus_tag="KQD82_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG4379930.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="KQD82_001455"
     CDS             32364..34097
                     /locus_tag="KQD82_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG4379931.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNITQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="KQD82_001456"
     CDS             34329..35540
                     /locus_tag="KQD82_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG4379932.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35732..35992
                     /locus_tag="KQD82_001457"
     CDS             35732..35992
                     /locus_tag="KQD82_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG4379933.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36346..38058
                     /gene="ptsP"
                     /locus_tag="KQD82_001458"
     CDS             36346..38058
                     /gene="ptsP"
                     /locus_tag="KQD82_001458"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG4379934.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLEN
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(38850..39515)
                     /locus_tag="KQD82_001459"
     CDS             complement(38850..39515)
                     /locus_tag="KQD82_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379935.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT        15785 a         4145 c         6911 g        12698 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaagataaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa atctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgaaata ttaagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 gatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacgcaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccatagtaca aacttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gccatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaacct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtccat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt aaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ttatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatagtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcgacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtact atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacctaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatctatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccga
    11461 taatacttac tttatttagt gtaattattg gtaaagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgagaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaggtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggctca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagatact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatatgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgattct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag atattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcttaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgcaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaaaacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttgta tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aatcatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggataattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcagctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta taaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctgtgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaaataat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtgctat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagtattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggtcagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tatttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtaac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactaatcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aatattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaatcttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agataatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtaaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataactcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataacaat ttttcaagtg
    33781 cagatgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttagag
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatataattc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaactg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaattt gtaattatac tcgaaaaaag
    35581 tgtatattag tagtataata tttttgtagc actagtggga acaaaaaatg accataggac
    35641 aaaaaagtac cacttagtac cattaaagta aaaaacagga aaacaatttt aaaaaatata
    35701 aaatgtaaag tttataggag gatttaaaaa aatggaaaaa gtagttagta tcaaaaatgc
    35761 aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg aatttaaatc
    35821 aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa tgggaataat
    35881 gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag aagatgaaga
    35941 agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat agatataata
    36001 attttttaaa aatttgatat ttagaatcta atattactta aagtaaagta acagagaagt
    36061 attaaacata tacagaccaa catttgtaga tatttaattt ctttgttact ttcatgttta
    36121 tttaatcaga agattaagac tattgttaat tgggtataaa aatataaata ttttagaaaa
    36181 tgaaataaga tatttttaat cataaattca gagtaaaaat acagtaacaa atagtttata
    36241 aaattttata aaaaattata agtaaataaa actataaaaa tgtaaattat ttataaaatt
    36301 ttatagagtt tatgtgctag atgatatttt ttaggaggta gggttatggc ttacaaagga
    36361 ataggtgctt ctcctggtgt tgctttaggg aaagcgttag tagttgaaca tagtgaatta
    36421 gtaattgaaa aaaaatctat agataatgtt gaagctgaaa ttgctaaatt agaaaatgca
    36481 gttgcagttt ctaaagaaga attagtaaaa gttaaggaaa aggcttctga agaattagga
    36541 gcagaggaag cagaaatatt tgaagcacat ttattagtct tagaagaccc agaattaatt
    36601 ggttctgcaa tagataaaat caaaactgaa agtgtaaatg cagagtatgc tttaaatgaa
    36661 ataaaagaga tgtttgttag catgtttgag tctatggaca atgagtacat gaaagaaaga
    36721 gcggcagata taaaagatgt tacaaataga atacttagac atatattagg aattaaagtt
    36781 gttgatttat ctgctttaag cgaagaagtt gtattaatag cacatgattt aactccatca
    36841 gacacagcaa ctatgaataa aaaaatggtt ttaggatttt taactgatat aggtggaaga
    36901 acttctcata ctgctatcat gtctagaact ttagaaatag ctgcaatcgt tggattaaat
    36961 gatataactt caaaagttaa ggatggagat tttgtagttt tcaatggaga cacaggtgaa
    37021 gtaatcgtga atcctgatga agagactata aataaatata ctgaattaaa agctaagtat
    37081 gaagatgaga gaaaagcatt acagctatta aaaggaaaac catctgtaac tttagatgga
    37141 aaacatgtag aattagctgg taacatagga acacctaacg atatagaagg tctaataaag
    37201 aatgatgctg aaggtgttgg tctttataga acagaatttt tatacatgga tagagattct
    37261 ttcccaacag aagaaataca atatgaagca tacaaagctg tattagaagg aatggatgga
    37321 aagcctatag ttataagaac attggatata ggtggagata aagaattaag ttatttaagt
    37381 atggaacctg aaatgaatcc attcttaggt tacagagcaa taagactttg cctagataga
    37441 aaagatatat ttaagactca attgagagct ttatatagag ctagtgttca tggaagatta
    37501 agaatcatgt tcccaatgat ttcatcttta gaagagttgt tacaagctaa agaagttgta
    37561 aaagaagttt tagcagaact tgattctgaa ggtgtggctt atgctaaaga tgtagaaatt
    37621 ggtatgatga tagaagttcc atctgctgct gtgatatctg atgtattggc taaacacgta
    37681 gatttcttct ctataggaac taatgattta attcaataca cttgtgcagt tgatagaatg
    37741 aatcaaaaga taagttatct ttacaatcaa tttaatccag ctgttttaag acttataaag
    37801 actgttatag acaatgcaca caaagaagga aaatgggcag gtatgtgtgg agaatctgct
    37861 ggagaccaaa aaatgatacc aatactttta ggtatgggat tagacgagtt ctctatgtct
    37921 ccaatatcaa tacttcctgc tagaaagtta ataacttcag ttaaagagtc agatatgaaa
    37981 aaattagctg atgatgtact taatatgggt actgctgaag aaataaaaag ctatatagaa
    38041 aagactttta atatataatt aatgttatat agttactaaa attcattttt ctaaataaca
    38101 aaattatatt ttacgaatta aaaagaagat gtttcaaaat aaaaatttga agcatctctt
    38161 ttttataaaa gattactaag aattacttaa tctttgtaat agtcattttg tgaaaaagca
    38221 aaactgcatt gtgactatag tatatatgga tgttattatc aataaaagta ctattaaaat
    38281 gaagattcat tctcaaatgg tatcaaaagt agttgtgatg gagtaagata atattttgtg
    38341 atttaatagt aaaaattaaa aaaatatggt attgaaaaat aattaaaagt atttaataat
    38401 agaattaggg aagaataagt ttataagtaa aaataattga ttagtagtta ataaaataaa
    38461 tgtgatatcc aaataaatcc taaatctaat ttgagtttta atatagatag tctaaagttt
    38521 gattattaaa gatttaattg taagttgtta cagttgatga tattgattag agattaaagt
    38581 ttagagtaga aagttacctt aaatttaaaa atgcaagata acccaatcta aaaattataa
    38641 tcactcgata ggtttaagga tttatttgtt tattataaat aggagggtgt ctcaaaatga
    38701 actttttagt ttatgaggca ctctctttta tatagaatta aatatatttt attatttcaa
    38761 caatgaaaaa gaagcttatc tctattttga gacagcctct ttgtatacta ttattaattt
    38821 aatcaataac ataatttaaa attatattat tagttaagat ttcctgcttt attagtaaat
    38881 aattctttaa tagcattgtt tctggaaact aaatcgttat cagagttgtt tgtttttttc
    38941 ataatcactg aataacgttt tttatttata aaaaatatta aaatcttatc atcatcaaaa
    39001 ttgtattctt tatatctgtc aatagactca taacctagat ttagaatgtt tttgtctttt
    39061 atactatcta catctaaaat atatgatggg tcagccttta gtaatatatt aattaaacat
    39121 ttttcttcac cagcacagaa taactccatc ttacttgata tagatttaga ttgctctgaa
    39181 gttatttttg caggaataag gttgtcatta tcatctctta gaagataacc atgtgtgtct
    39241 tgttgatata ctaaattaac ctttttctta ggaacactaa tcattttatc taaatgtaca
    39301 taatgatgat tttcaacaaa accttcattt aatatttcaa caaagttatt aattttacct
    39361 aggcaaaaag attctataga ttctttacag aatttatttg taaatggtat tatgtttacg
    39421 ttttcctcgt ctgtattcca tgctatagca taatccaacg aatatacttc ttcctctata
    39481 tctgcaaaat tatattgtac tatgtttcct ttcaaaatta ctcgacctcc atcttataa
//
LOCUS       DAENCV010000030        39530 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAENCV010000030 DAENCV010000000
VERSION     DAENCV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.030"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            223..2091
                     /locus_tag="KQD82_001460"
     CDS             223..2091
                     /locus_tag="KQD82_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4379936.1"
                     /translation="MEKSSIKILIGLFIIGVIIILIFLNRTKTKNYLYQIFNVIINST
                     KLIFNYSWIYIFLCIIFSVFQGFFPIISLLIMQKMINAIQMKNIGQDIFLKLIFIYIV
                     LEMIDLMAVEIYNLYSTTFMKRFTRHIEILMLEKANRLTTKDFENKEIYDLINRAQSQ
                     NGATVIDYFNSWILIFQQAITIFSSIIIIISYQYWIVILIIIAPVIQFYYSQKLGHEQ
                     YEISMKRTSEERKCWYINFLMLLGNSIKETILYGLGNLFINQYGKLKEKFILQDYKIM
                     KKTFEMNFIIIIIGQLFTGVIYFHILLNGFWQKIYMGDVTTYITCITTIKNGIEVILS
                     MMSTIYSKSLYMELLFRFFDIPEKTELNKTKLKIDNIEKIELKHLYYKFTEDSGYVLK
                     DINLELKKNSPIALVGRNGSGKSTLIKIILGIYDDYEGDILINGINLHNIEKENYYNK
                     ISCVFQDYTKYEASVKENVGYGNLDQINNEDLIKESLIKSGLDINQLGSNKLNTILGY
                     WFGENQLSEGQWQKIAISRAMMKDADFYILDEPDAALDGIAENDMLLTYKKMFENKFG
                     IFVSHKTHHLNLLCENIIVLNNGQIAECGNHEELIEKEGIYHQLYSSQIISKLETN"
     gene            2120..3547
                     /gene="ccpM"
                     /locus_tag="KQD82_001461"
     CDS             2120..3547
                     /gene="ccpM"
                     /locus_tag="KQD82_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG4379937.1"
                     /translation="MEQKPFIHLFQTSTGFYFYDVNTDSILNIDENVYDYLKKEQLGE
                     SSSNTLEYVEFLRKQGYLKSNKVKITEHPETEVLKYHCENRVEGIILQVTQNCNLHCD
                     YCTYSGGYINRIHTNKRMSKETAKRGIDYLISHSRDCQYVSIGFYGGEPLLEFDLIKW
                     CIEYSKANIEGKNLKYNLTTNATLITEEIIELFEENNVSIMISLDGPAEIHDKNRKFA
                     NNNVGSFSTIMKKLDMISTKYPNYFKNNVHFNSVLATDNFSCVNKFFTKEELFQNSLF
                     LSSIVSEVNAKVKTEKSQSFYEESQYALFIGYLTLLGRIKKEDSSKLLQTQISTIGDT
                     RNGKQGKQRLVMPEKWHHGGPCVPGVMRLFINVDGYFYPCEKASEASDSMNIGNLDQG
                     YDLKKVEKLLNIERINGDKCHSCWAYENCRICAVSLSVDSTQEDIAKECEYIRSSIES
                     DFQDYCVLKELGFDFETKELIENTI"
     gene            3610..4686
                     /locus_tag="KQD82_001462"
     CDS             3610..4686
                     /locus_tag="KQD82_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG4379938.1"
                     /translation="MKKVLIYPFTEEMAPFVKQENLLKDIDITSIVSLKGNGILGDKY
                     KFNGKSLEVSENFDKELEKCTYILLNNFYSDESENIILENIKKAVNHGKKIIFFSNSN
                     SNIDNLKMIIPKEQQFEINSSKNMVKDFQGRIYNIDTPVIFVCSIFEGLEKFDIQLAL
                     RNKLLIDGYKVLQIGTRQVCELFDFYSFPEFMYSNNIEEQDKVLMLNNTLKKLELEKK
                     PDLIIIGIPGGILPFSSKAFGDFGITMYKVLQAVTPDCTILSIPHGKYSDEEISYFGK
                     ITKSKFNIEVDYYNIAAKSILSHPTETMGKPFYLTLGKSLVEKKIATLQNKNVFCLSN
                     KSEIDRLIELMIKEFIKYGTVASI"
     gene            4720..5010
                     /locus_tag="KQD82_001463"
     CDS             4720..5010
                     /locus_tag="KQD82_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG4379939.1"
                     /translation="MVNTEVMNKLDIYTVLVNIFEKRSKINFDENCYLKNENLFESKI
                     MMPARELVLTLYDIEDYFSISIPEDVILSGRFNSFNNIYDIVKKLENSINFN"
     gene            5052..5270
                     /locus_tag="KQD82_001464"
     CDS             5052..5270
                     /locus_tag="KQD82_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379940.1"
                     /translation="MSNFGKRNLYDSANGEIDPKGGCNCGALCANLCSCSCSGGTWSF
                     YHTNQVKGSKINNGQQFRSPGEIPGQKK"
     gene            complement(5474..7141)
                     /locus_tag="KQD82_001465"
     CDS             complement(5474..7141)
                     /locus_tag="KQD82_001465"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG4379941.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNSY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(7338..8060)
                     /locus_tag="KQD82_001466"
     CDS             complement(7338..8060)
                     /locus_tag="KQD82_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4379942.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            8451..9878
                     /locus_tag="KQD82_001467"
     CDS             8451..9878
                     /locus_tag="KQD82_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4379943.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            9871..10650
                     /locus_tag="KQD82_001468"
     CDS             9871..10650
                     /locus_tag="KQD82_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG4379944.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            10913..11638
                     /locus_tag="KQD82_001469"
     CDS             10913..11638
                     /locus_tag="KQD82_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4379945.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            11768..13708
                     /gene="mngA"
                     /locus_tag="KQD82_001470"
     CDS             11768..13708
                     /gene="mngA"
                     /locus_tag="KQD82_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG4379946.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIHKKIRY"
     gene            13837..16452
                     /gene="mngB"
                     /locus_tag="KQD82_001471"
     CDS             13837..16452
                     /gene="mngB"
                     /locus_tag="KQD82_001471"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG4379947.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEIGHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNFV
                     NVECLMNQVKTILVK"
     gene            16712..17866
                     /locus_tag="KQD82_001472"
     CDS             16712..17866
                     /locus_tag="KQD82_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG4379948.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKSYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTSTTTQIFNLITSIQQSKNM"
     gene            complement(17989..19902)
                     /locus_tag="KQD82_001473"
     CDS             complement(17989..19902)
                     /locus_tag="KQD82_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4379949.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDDHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDKKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            20144..20461
                     /locus_tag="KQD82_001474"
     CDS             20144..20461
                     /locus_tag="KQD82_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4379950.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            20469..21716
                     /locus_tag="KQD82_001475"
     CDS             20469..21716
                     /locus_tag="KQD82_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4379951.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTIVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            21745..22098
                     /locus_tag="KQD82_001476"
     CDS             21745..22098
                     /locus_tag="KQD82_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4379952.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEADGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            22120..23565
                     /locus_tag="KQD82_001477"
     CDS             22120..23565
                     /locus_tag="KQD82_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG4379953.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVIEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            23957..24709
                     /locus_tag="KQD82_001478"
     CDS             23957..24709
                     /locus_tag="KQD82_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4379954.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            24811..25731
                     /locus_tag="KQD82_001479"
     CDS             24811..25731
                     /locus_tag="KQD82_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG4379955.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            25734..27650
                     /locus_tag="KQD82_001480"
     CDS             25734..27650
                     /locus_tag="KQD82_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG4379956.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            27670..28539
                     /gene="gatY"
                     /locus_tag="KQD82_001481"
     CDS             27670..28539
                     /gene="gatY"
                     /locus_tag="KQD82_001481"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG4379957.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            29049..30512
                     /locus_tag="KQD82_001482"
     CDS             29049..30512
                     /locus_tag="KQD82_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG4379958.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
     gene            30516..31700
                     /locus_tag="KQD82_001483"
     CDS             30516..31700
                     /locus_tag="KQD82_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG4379959.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            31733..32041
                     /locus_tag="KQD82_001484"
     CDS             31733..32041
                     /locus_tag="KQD82_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379960.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            32074..32454
                     /locus_tag="KQD82_001485"
     CDS             32074..32454
                     /locus_tag="KQD82_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG4379961.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            32444..32872
                     /locus_tag="KQD82_001486"
     CDS             32444..32872
                     /locus_tag="KQD82_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379962.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            32921..36256
                     /locus_tag="KQD82_001487"
     CDS             32921..36256
                     /locus_tag="KQD82_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG4379963.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLYGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEYRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            36637..38700
                     /locus_tag="KQD82_001488"
     CDS             36637..38700
                     /locus_tag="KQD82_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG4379964.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(38917..39483)
                     /locus_tag="KQD82_001489"
     CDS             complement(38917..39483)
                     /locus_tag="KQD82_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG4379965.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
BASE COUNT        15595 a         4232 c         6844 g        12859 t
ORIGIN      
        1 taaatttaaa ttgttgttga aatttttttg atttcatgct accattgtag tataaataat
       61 gattagaaac gtttcgagtt aattactatt tattttaaac tatgtcttaa ataaaatata
      121 tatttattgt tatataataa gcaataaatt gcttgataat atatttttat gtgaaaaaaa
      181 catcaaaaaa atacttttaa gaatttaatt tcaggtgaaa taatggagaa atcatcaatt
      241 aaaatattaa ttggtttatt tattatagga gtaattatta ttttaatctt ccttaataga
      301 actaaaacta aaaattatct ttatcaaatt tttaatgtta taataaacag tactaaatta
      361 atctttaatt atagctggat atatattttt ttatgcatta ttttttcagt atttcaaggt
      421 ttttttccaa taatatcttt gttaataatg caaaaaatga taaatgcaat tcagatgaaa
      481 aatatagggc aagatatctt tttaaaatta atttttatct atatagtctt agagatgatt
      541 gatttaatgg ctgtagaaat ttataattta tactctacga catttatgaa aaggtttacc
      601 agacatatag agattttaat gttagaaaaa gctaatagat taactacgaa ggattttgaa
      661 aataaggaaa tttatgattt aatcaataga gctcaatctc aaaatggagc tacagttatt
      721 gattacttta actcctggat tttaattttt caacaggcaa tcacaatatt ttcctcgatt
      781 attataatta tatcttatca atattggatt gtaatattaa tcataatagc cccagttata
      841 caattttatt actctcaaaa attagggcat gagcagtatg aaataagtat gaaaagaact
      901 tctgaagaaa gaaaatgttg gtatattaat tttttaatgc tattaggaaa ttcaatcaaa
      961 gaaacaattt tatatggatt aggaaattta tttataaatc agtatggaaa attaaaagaa
     1021 aaattcattc ttcaagatta taaaataatg aaaaaaacat ttgagatgaa ttttataatt
     1081 ataattattg gtcaattatt tacaggagtt atatattttc atatacttct taatgggttt
     1141 tggcagaaaa tatatatggg agatgtaact acttacataa cctgtataac aacaataaaa
     1201 aatggtatag aagtgatatt atcaatgatg agtacaatat atagtaaatc tctatatatg
     1261 gaactattat ttagattttt tgatattcct gagaagacgg aacttaataa aacaaaactt
     1321 aaaatagata atatagaaaa aatagagcta aaacatttat attataaatt tactgaagat
     1381 tcaggttatg ttctaaagga tattaatcta gaactaaaaa agaatagtcc tattgcactt
     1441 gttggtagaa acggttctgg taaatcaact ttaatcaaaa ttatactagg aatttatgat
     1501 gattatgaag gagatatctt aataaatgga attaacctac ataatatcga aaaagaaaat
     1561 tactacaata aaataagttg tgtttttcaa gactatacca aatatgaagc tagtgtaaaa
     1621 gaaaatgtag gatatggaaa cctagatcag ataaataatg aggatttgat aaaagagtca
     1681 ttgataaaat ctggactaga tattaatcaa ttggggagta ataaattaaa taccatttta
     1741 ggatattggt ttggagaaaa tcaactttca gaaggccaat ggcagaaaat agcaatatca
     1801 agagctatga tgaaagatgc tgatttttac attttagatg aaccagatgc cgctttagat
     1861 ggaatagctg aaaatgatat gcttttaact tataaaaaaa tgtttgaaaa taaatttggt
     1921 atattcgtat ctcataaaac acatcattta aatttgttat gtgaaaatat aatagtttta
     1981 aataatggac aaattgcaga atgtggaaat catgaggaat taatcgaaaa agaaggaatt
     2041 tatcatcagt tatattcaag tcaaattata agtaaactag aaacaaatta ataattactt
     2101 tgaaaaggag ttaagaatta tggaacaaaa accttttatt catttatttc aaacatcaac
     2161 tgggttctat ttttatgatg tgaatactga ttctatatta aatatagatg aaaatgtcta
     2221 tgattatcta aagaaagaac aacttggaga gtcaagtagt aatacattag aatatgtaga
     2281 atttttaaga aagcaaggat acttaaaatc aaataaagtg aaaataacag aacatccaga
     2341 aactgaagta ttaaagtatc attgtgaaaa tagggtagaa ggaataatat tacaagtaac
     2401 tcaaaattgt aacttacatt gtgattattg tacttattct ggaggatata taaatagaat
     2461 acatacaaac aaaagaatgt ctaaagagac tgctaaaaga ggaattgatt atttaataag
     2521 ccattcaaga gattgtcaat atgtgagtat aggtttttat ggcggagagc cattactaga
     2581 atttgattta attaaatggt gtattgaata ttcaaaagcg aatatagaag gtaaaaattt
     2641 gaaatacaat ttaacaacta atgcaactct tataacagaa gaaattatag agttatttga
     2701 agaaaataat gttagtatta tgattagtct agatggtcct gctgaaatac atgataaaaa
     2761 tagaaagttt gcaaataata atgtaggatc ttttagtact ataatgaaaa aactagatat
     2821 gatatctact aagtatccaa attattttaa aaataatgta cattttaatt ctgtattagc
     2881 aactgataac ttctcttgcg ttaataaatt ctttactaaa gaagaattat ttcaaaattc
     2941 attattttta tcttcaattg ttagtgaagt aaatgctaaa gttaaaactg agaaatcaca
     3001 aagtttttat gaagaaagtc agtatgcttt attcatagga tatttaactt tacttggtag
     3061 aataaaaaaa gaggatagtt ctaaattatt acaaactcaa atctctacta ttggagatac
     3121 gcgaaatggt aaacagggca aacaacgttt agtaatgcca gaaaaatggc atcatggggg
     3181 tccatgtgta ccaggagtta tgagattatt cataaatgtt gatggttatt tttatccatg
     3241 tgaaaaagca tcagaagcat cagatagtat gaatatagga aatttagatc aaggatatga
     3301 ccttaaaaaa gtagaaaaat tattgaatat tgaaagaata aatggtgata aatgtcatag
     3361 ttgttgggct tatgaaaact gcagaatttg tgctgtttct ttaagtgttg attcaacaca
     3421 agaagatata gcaaaggagt gtgaatatat tagaagtagt atagaaagtg atttccaaga
     3481 ttattgcgta ttaaaggaat tgggatttga ttttgaaaca aaagaactta tagaaaatac
     3541 catttaattt aagatagcat cttaaattat tacattatga aactaattag aatctattgg
     3601 aggattaaaa tgaaaaaagt attaatttat ccattcacag aggaaatggc tccatttgtt
     3661 aaacaagaga atttactaaa agatatagat ataacatcaa ttgtaagttt aaaggggaat
     3721 ggaatcttag gagataaata taaatttaat ggtaagtctt tagaagtttc tgaaaatttt
     3781 gataaggaat tagaaaaatg tacatatatc ttacttaata atttttatag tgatgaaagc
     3841 gaaaatatta ttttagaaaa tataaaaaaa gctgtaaatc atggtaaaaa aataatcttt
     3901 tttagtaatt caaatagtaa tattgataat ttaaaaatga ttatacctaa agaacaacag
     3961 tttgaaatta atagttcaaa aaatatggta aaagattttc aaggaagaat atacaatatt
     4021 gatacacctg ttatttttgt atgtagcata tttgaaggct tagaaaaatt tgatattcaa
     4081 ttagcattaa gaaataagtt actaattgat ggatataagg ttcttcaaat tggaacacgt
     4141 caagtttgcg aattatttga tttctactca ttccctgagt ttatgtactc aaataatata
     4201 gaagaacaag acaaggtact aatgcttaat aatactctaa aaaaattaga attagaaaaa
     4261 aaacctgacc ttataattat cggaattcca ggtggtatac ttccattttc ttcgaaagca
     4321 tttggtgatt ttggaattac aatgtacaaa gtacttcaag cagtaactcc agactgtaca
     4381 atactatcta ttccacatgg taaatatagt gatgaagaaa tttcttattt tggtaaaatt
     4441 acaaaaagta agtttaatat agaggtagat tattacaata ttgctgcgaa atcaatatta
     4501 tctcacccaa cagagactat gggaaaacca ttttatttaa cattaggaaa atcattagtc
     4561 gagaaaaaaa tagccacatt acagaataaa aatgtatttt gtttatctaa caaatctgaa
     4621 atagacagat taatagaatt aatgatcaaa gaatttataa aatatggtac tgtagcatct
     4681 atataaaata ttttacataa aggatggtga aatactgata tggtaaacac agaagttatg
     4741 aataaactag atatctatac agttttagta aatatatttg agaaaagatc aaaaataaat
     4801 tttgatgaaa attgttattt aaaaaatgaa aatttatttg aatctaagat aatgatgcct
     4861 gctcgtgaac tagtattaac attgtatgat atagaagact atttttctat atctattcca
     4921 gaggatgtaa ttttatcagg aagatttaat tcttttaaca atatttatga tattgttaaa
     4981 aaacttgaga attctattaa ttttaattaa ttaaaatata ataatataac aaatgagagg
     5041 ggaaaaatga tatgtctaat tttggaaaaa gaaatttata tgattctgca aatggagaaa
     5101 tagatcctaa aggaggatgt aattgtggtg ctctgtgtgc aaatctatgc tcttgtagtt
     5161 gttctggggg aacatggtca ttctatcaca caaatcaagt gaaaggttct aaaataaata
     5221 atggtcaaca atttagatca ccaggagaaa tacctggaca aaaaaaataa aaagagttaa
     5281 atataattag ttttagcttt atcaattaac ataattaact ttattaaaat taatagaatc
     5341 taattacaat tgtttattat atctataaaa gcaactttaa aataccttgt tttatatatg
     5401 ttaattaaaa atggtaggag ctgtcgtgct agaaaatagt gctacagctc ctaccatttt
     5461 tacataaata actttattta taatatatca ttatactttc gtaaggtttt atttccattt
     5521 tattttcaat tatatctcta gtgtaattgg tttcaagtat tgtgtagttt aatttattca
     5581 aataagtttc aggtaattta tatactacat tatcatgagt aaaattactt ataacaagaa
     5641 gattttggtt tttatactct ctttcataaa caaatagatt tttatgattt tcatctaaca
     5701 atctataagt accttctgta aaaatatcat attgtttacg atatttgatt aatctttgat
     5761 aagtataaaa gactgaacga gaatcattaa gctcattttc tacactaata ttgtcattat
     5821 taacttttaa ccacggttta tgattagaaa accccttata aatagtatta tcccattgca
     5881 taggagtacg ggcattatct ctggattttt gttgaagtat aagcaatgcc tccttttcat
     5941 caataccaga tttaatcata tgatagtatg aattcttact ttcagcatca tcatattcat
     6001 ctattttagt aaagtaggca tttgtcatac ctatttcttc accttgatat atgtatgtag
     6061 taccttgtag accaaatagt gttatggcta acatcttagc actttcttga cgaaaaaatc
     6121 tatcatctcc aaatcgagat aaagctctag gttggtcgtg attagagtaa aatagagcat
     6181 tccatccctt tccttcatac attttatatt gaaagtcaga ttgtgctttt tttaatttat
     6241 ccagagagaa catttcatta gtccattttg cgcctccttt ataatctgtt ttcatatgat
     6301 ggaagctaaa aaccatagat agttcttctc gattttctct agtatagttg atacactctt
     6361 caggtgtagt agatgacatt tcacctacag taactatatt tttaggtttg aaaacttttt
     6421 gattcatatt atgtaaatat tcgtgtatac gaggaccatc agcgtagtga gttcttccat
     6481 ctccttttgg tcctgttata gggtcatcag gtaatccagg tgttttggag agtaggttga
     6541 ctacatctaa acgaaaacca tcaatacctt tatcagccca aaattctagt attttgtaac
     6601 attcttcacg aaccttttca ttttcccaat ttaaatcagc ttgagtttct tcaaataaat
     6661 gtaaataata ttggttagtg gtttcatcat atttccaagc agttccacta aatctggata
     6721 tccagttatt aggaacagaa ccatcaggtt tagcatcttt ccagaaataa taatcacgat
     6781 atggattgtc tttgcttttt ttagattctt taaaccattt atgttcagta gatgtatggt
     6841 ttaggaccat atccatcatt atttttatgt ctcttttatg agcttctttt aagagttttt
     6901 caaagtcatt cattgtccca tattttgggt ctatattata ataatcttct atatcatatc
     6961 cattatctct ttgaggagaa acgtacatag gagtcaacca taataaatca acacctaaag
     7021 aatacagata atctaatttt tctataatac catttatatc acctatacca tcattgttgc
     7081 tatctttgaa actttttgga tatatttgat agacagttgc ttttttccac caatttttca
     7141 tacttgatac ctcctgtaat ttataagtta attataatat gtttaaacaa attgtcaagt
     7201 attaaactga tattaaagct aaaatataca ataaataatt atttgtataa acatataatt
     7261 gtatatagaa tagtgaggtt agtatgggat aaagtgtcta aacgtaaaaa aattatgttt
     7321 aattaaacat aattttttta atatctttga gtaacatcca tgaatacaaa tgtttcaggt
     7381 gtatgctttg attcagtata ttcaaaaagc tttccatcat cagtatatac actattttta
     7441 ataacaccaa cacaattata atcatttaga ggtagcgttt ttaactcaag ctctgtagct
     7501 ttttcaattt taaatatctt tctggatgaa acaatttttg tacctagaca ctcttctata
     7561 tatttataaa tagagccttg tgctatagaa acatcaaggt ctttaactat actacataga
     7621 aaatagttaa tgtcgagaat tttaggtatg ttatcaatat atcttagtct gtgaaggtaa
     7681 tatacttcac taccaacttt aaaacctgtc tttttagata acttattatc aattaagatt
     7741 ttgctgaagt ggagaacact agtagcagta ttaacctttt tatcttctac aatcgattct
     7801 acacctttga aattgtgcat atttagaaaa aaatcattag aacaactatt ttctaacact
     7861 acaacacctt tgccttttat actttgaaca tatccttctg tgtttaattg agaaattgct
     7921 ctacgtacag tgtttctgct acatgaaaac ctttcaataa gttgaagttc agtaggcagc
     7981 atcatattac ttgtatattt accatcaagt atttcctctt tcaatacttc atatatctct
     8041 gaaaatttat ttttagccat atacccctat ccctccttaa aggtctttag taatcttatt
     8101 gaactgatat gaacaaatta tatcaaatgt gtttagacat gtatagtgaa aatagaatca
     8161 tatcaaccat aaaaaggaca aaatagaatt attttgtggg ttgtttcaaa tacatggtta
     8221 aacatatttt taaaataaaa aaaatgaatt aaaaaaatag gctagatata gcaatacact
     8281 taaaaggatt tatatgttaa accaaatgat aaaaataaaa tgaataaaaa atattgacaa
     8341 tttgtttaaa caaatataat atttaaaata taaaatgtga ataaatcgtt ttcataaaaa
     8401 attaaaccga aatagaaagg ggattgtata tgaagaggtt cttaaacaaa atgatgcaac
     8461 aactgtcact tttaggaaag actatgctgg tgccaatagc tgtaatgcca gcagcaggta
     8521 tattaggatt attatttggt ccaaatatgc tcaatgtgcc agctatatct aatatatcta
     8581 atatagtatt ttcaaatgtg gactacttat ttttattagg agcagctagt gcctactcaa
     8641 agtctaaaga caagacaagt gtaatggttg cagccgtagt tgcatatatg atatttaagt
     8701 caaatttaga gttattgaat gatacattaa atgcaggtgt atttggtggg ataattgttg
     8761 gagcttgtat agcatggact tacaataaaa catataaagt taaagttcca gcatttttaa
     8821 gcttctttgc aggagaaaaa tgtgtaatta cactatcacc agtggttgct ttaattttat
     8881 catatatatt tagtttatta tggatatatc cacaagctgc aatgaatcaa tttggttttt
     8941 ggttgggagg agctggtgca ataggaatat tcttgttttt attctgtaac cgtgcactaa
     9001 taccaactgg gttacatcaa gttttaaatg cttacatatt atttgaaatg ggtgaatata
     9061 caacatcaac aggagaagtt ataagaggag aaattcctag atttatggca ggagacccaa
     9121 atgcaggatt cttctggaca ggattttaca taattatgat gttttcaata cctgctattt
     9181 cttatgctat atataaaaca gcacaagata aaaagaagca agaagtaaaa ggtgcaatga
     9241 ccgctggagc attgacttct tttatggcaa cagtaactga accaatagaa ttttcgtttt
     9301 tatttgcatc tccaaagtta tatataattc attcttttta ttctggtcta ggtggagttg
     9361 tactatattt actgggtgct cgcttgggtt catttaatgg agcaagtata attgactatg
     9421 tcttaagttt tagttatgga gataaagcgt ggttagttat acctgtagga gttgtattct
     9481 ttgtattata ttatgtaaca tttaaatata taataataaa agataatgtg caaacaccag
     9541 gacgtgaagt tgagatagag ataggtgata aagttagtga aaaagaaaag aatttaaaac
     9601 tttctcatgg taatttttca tatatggcta aacagataat taaaaattgt ggtggttatg
     9661 aaaatatagt gactcttaat aattgtatga cgagattacg tttagaagtg aaggatgcta
     9721 ctattttgaa tgatgataat attaaaaaaa caggagccaa aggggtaatt aaattgagta
     9781 atacatcagt tcaaatcatc ataggaacgg atgttgttaa agtaaaggat gagatggaaa
     9841 tgcaacttga tgagttgaga aagcaggcag atgcataaaa tgaagaaatt agtagttagg
     9901 gcagatgata taggatattc agaaggtgtt acactaggga taatatcttc ttataagaac
     9961 ggtattgtta caagtgctgg aatcatggtg aatatgcctt tttcaagaca agctatagaa
    10021 atggtaaaaa atgatgatgt atgttttggt ttacatgtta atttagtaat aggagaacct
    10081 tgttgcaaag aacatattaa gataaagaat ttgttagatg aaggtggaaa atttgtttct
    10141 agcaaaataa gaagaacgca actactgaca ggtaaagagg tttttaatta tgaagaagta
    10201 tatgaagaag tttgtgctca aattgaaact tacaagttgt atatgagtaa attgcctgag
    10261 tatattgata tacatggtat agaagaagat gtatcaatat cagcaatgtg tgatgctgca
    10321 aaagaatacg gtataccaag ttgtccctat tatttagatt cgtgtattag catgccaagt
    10381 aaatatcctc agaatgattt ttacaagtta aataaatctt ttgttaaatt atttgaagaa
    10441 aaatatataa atttagataa agatataaca ttgatggtta ctcatccagg atttatagat
    10501 tatagtctta taaaatcatc ttctcttatt caagagagag tatatgaaca tgcaatgcta
    10561 tgtgataagg aagttaaaat gtggttaaaa agaaataata ttgaattggt tgattttaaa
    10621 gatattttca agtctaataa ttatatataa agaatagatt acctaaagtt ttaattaaaa
    10681 ataagtattt gaaaaaaatg tgtattttct aaaaaaatat tgatagagat gtatcaaaat
    10741 aaaaacttga atcatctcta tcaatatagg aagtacacat tttttatgta taataaaaag
    10801 agtagtatgc attaataaat taaaaatatt aaattataat tatattaacg aaaattttag
    10861 ctaatatttt tgaattttac ttatgaataa taaaatagat ggaggagaaa ctatggaatt
    10921 ttatgaaaga gcaaaaaaat atgagtataa atttacagat acagaagaca tgataattga
    10981 atatatgaga aaaaacaaag aagaaatagt aaaattatca atacaatctt tagcagataa
    11041 attttttact gtcccaaata ccatagttag gctatgtaaa aaaatggagt atgatggttt
    11101 ctctcaacta aaaaataatt taaaagaaga gttaaaaaat gagaataaag agccaaaaaa
    11161 tggttttgaa tcagatataa aaaagacatt agagctaata gattatgaaa aaataaataa
    11221 agtagcagat ataattcaaa aatctaggag tattatagtt tatggaatag gtcaaaacat
    11281 tcctttatgt gagatattag taaaagaaat tagaggtggg catgataata taacttacta
    11341 tagtcaaaga catgaagtac ttcatgatat aggaaaatta ggtgaacaag atatgttttt
    11401 tgcacttagt ttaagtggag aaacgaaaga agttatagaa gcagcaaaga cagcagttaa
    11461 taagggttgt aaggtagtag ctctaacaca tatatgtgaa aatcaattgc aacaaattgc
    11521 agatataagt ttatattgct attcaacaat taaacaagta aatgggtaca atatatcaga
    11581 taaggttcca attatgatga ttatgagagc aataatagaa caatactggt taagatagag
    11641 atttgtgtat aaatacacaa atctttattt tttaagaaga aattgtgtgt ataatttcaa
    11701 aaaaacattt tcataattta ttttatgcta tattcatagc attaaatgat ttaaggaggg
    11761 cgtaattttg gaatttaaga aaatcacaaa tgaaaacctt gtttttttaa aacaaaaaat
    11821 aaaaacaaag aaagacgcaa taaagttctt agcaagaaag ctatatgatg aaggcaagtt
    11881 aacatcttat gaggaatatt tagaagctgt aatgtacaga gaaactttat ctccaacagg
    11941 ttttgaaaga gggcttgcaa ttcctcatgg aaaatctaag tcagttaaag aagctgcatt
    12001 tgcagctatg accttagaga caccaataga aaattgggaa agcgtagacc caaataataa
    12061 agtagagata atatttttaa tagcagtacc agaaaatgaa ggtggaaata cacacataca
    12121 aatactttct gagttagtta ctagactgtc aaatgaagat tatatgaata tgttgttaaa
    12181 ttcaaaaact aagaaagagt tatttaataa cttagatata aaagaaaaga aagaacaaat
    12241 aaaagaagag attaggattc aaaatgaatc aaaaaaaatt atattagcaa taacagcatg
    12301 ccctgcaggt atagcacata catatatgtc agcagaagca ttaattaaag ctggtaaaga
    12361 aatcggtgtg gatgtgtatg tagaaaagca aggagctaat ggtatggtgg accctcatac
    12421 accagatata ataaaaaggg cagatgcaat aatatatgca acagatgtag cacctaaaaa
    12481 tacagaaaga tttgaacatt tgccaaacat aaaaacgtca gtagcagctc cactaaaaag
    12541 agcaaaagaa ataatatacg aagctttaga agtagcacaa aaacaaggta aaggtgatta
    12601 tatagaaaaa tcatctgata tatcatattg tgaaaaatca tcatggaaaa aagatgtaaa
    12661 atcagcaata cttacaggag tatctcatat aataccactt attgtggcag gaggtatgat
    12721 acttgcattt gcagtattag catctcaagc atttggtcta caagaactat acaaccaaga
    12781 aggttcttgg ttatggcaat taagaaagct ttctggaggt atgttaggta cattgatggt
    12841 tccagtatta tcagcatata tgtcttactc actagctgaa aaaccaggtc ttggaccagg
    12901 atttgcagca ggtctagcag ctgatttaat aggtagtgga tttcttggag gtatgttagg
    12961 aggtttactt gctggttata tagtaaaatt tttaaagaag aatataccag ccaaaggcac
    13021 attagctgga ttcgtaagtt tttgggtata tccagtagtt ggttctttgg tagtaggttt
    13081 attgatgtta tttgtagtag gaaagcctgt agcagcactt aacaatggtc ttataaattg
    13141 gttaaatggg atgactggag taaatgcaat actactaggt gcaataatag gagctatggt
    13201 ttcatttgac ttaggtggac caataaataa agcagcctat acatttgcaa taggagcaat
    13261 agcaagtgga aattttatac cttatgcaat atttgcatca gttaagatgg tttcagcttt
    13321 tggagttaca atagctactg ttttaggcag agatttattt tcagaatcag aaaaggaaat
    13381 aggaaagcaa acttggatat taggattggc tggaataact gaaggtgcaa taccatttat
    13441 gatagaggac ccaatccgtg ttataccatc attggtagct ggttcagcca taactgggtc
    13501 tatagtagca ttcttcaata taggtcttca agtgcctgga gcggggatat tctcattggc
    13561 gcttttagag ggaagtatgt caggatttat tatagcatca atatggttct ttgcggcagt
    13621 acttggagct ataatatcag caatattatt aataataact aggaaacaaa aaaatcaaag
    13681 aaagatacat aaaaagatac gttattagat atttaaatca aaggtttaat tatagtgata
    13741 aaataaatat taaaaaattt tgtaaactca gtagtatttt tattggattt ttaattagac
    13801 agaaaatatt atcagataag ttttggagga taagcaatga aaaaaaatgt acacatagtt
    13861 cctcatatgc attgggatag agaatggtac ttctcaactg aggaatctag aatattactt
    13921 gtaaataata tggaagaaat aatggatatg ttagaaaata atcataacta tccatattat
    13981 gtgatggatg gtcaaactgc tatattagaa gattatttag cagttaagcc tgaatgtaag
    14041 gagagaataa aaaaattagt tcaagaagga aggctaataa taggtccttg gtatactcaa
    14101 actgatgaaa tggtagttgg tggagaatcc atacttagaa atttactata tggaataaaa
    14161 gattgtgatg aatttggaga atatatgaaa ataggatatt taccagattc atttggtcaa
    14221 agtgctcaaa tgccacaaat actaaatggg tttgatataa aatactcaat gttttggaga
    14281 ggatgttctg aaagaaaagg aactaataaa acagagttta attggaagag tgatgatggg
    14341 tcttctgttt tggttcaaat acttccatta ggttatgcaa taggaaaata tctaccaatg
    14401 aatgaagatg agctaagaac aagaatggat aaatatttac cggttcttga taagggtgca
    14461 actactgacc atataatact tccaaatgga catgaccaga tgccaattca aaagaatata
    14521 tttgaagtta tatataaatt aaaagaatgt tatccagaga gaaaattttt cttaagtaga
    14581 tatgaaaaca tatttaaaga gctagaaaaa aatgaggata tagatacaat aaaaggggaa
    14641 tttttagatg ggaaatatat gagggttcat agaagtatat tctcaacaag aatggatata
    14701 aaatcagcaa atgctagaat agaatcaaag ataaccaaca tattggagcc actagcttct
    14761 attgcatatt ctctaggatt tgaatatcat catggattaa tagagttaat ttggaaagaa
    14821 ataatgaaaa atcatgctca tgacagcatt ggatgttgtt gttcagataa agttcaccat
    14881 gaaataatga atagattttt cttagctgag gaaaaagtag accaattaat aacattttac
    14941 aaaagaagaa tagttgatgc aatgagttgt gaaatggctt tagataagtt agtagcattt
    15001 aacttaatgc cttacgatag gaatgaaata gtgaacgccc aagttataac taaaatgaaa
    15061 gcatttgaaa tatttgataa agataataat aagttggatt ttgaagtggt acataaagaa
    15121 gtaatagatg caggattaat agaccgtcaa attgttcatt atggaaacta tgattcattt
    15181 atggtatata caataaactt taaagataaa atacctgcaa tggggtataa ggcgtatatg
    15241 ataaaagaaa ttggacatat gttagaaaaa gcttatgaag tatgtgacat ggtagataat
    15301 gacttttata ctattcaagt taatgaaaac gggacattaa agatatttga taaaaaatta
    15361 aataaaactt ttgaaaatgt attattgatg gaaaatggag gagatgaagg agatgagtat
    15421 gatttttcac cacttccaaa tgagaaatta atatttaacg ataatatagt agcaaattcc
    15481 actataaaga aaaatagatt taataatgag ataaagataa attatagatt aaaagtacca
    15541 aaagatatac aagctagaaa aaataataga gcagattcat ttattgactt tgatatagtt
    15601 ataaatgttc caaatgataa agctataata gatgtagtat tcaacataaa taaccaagca
    15661 agagattgta gggttagaac ttatatacca acaaatatag cttcaaagtt ttctgtgtct
    15721 gataatcagt ttggacatat aaagagaagt gtgtatgatg atgctatgga agtttggcaa
    15781 caggaagaat ggagtgaaag accagattct atatatccaa tgctaacatt cgttggattg
    15841 tctgacaaag agcatggagt agctgttctt acaaacagta caagagagtt tgaaatagta
    15901 ggggaaaaat ttgacacaat agctataaca ttatttagaa gtgtaggatt cctaggtaaa
    15961 gaagaaatgg caagacgccc aggtcgtcca tcaggaataa agttgcctac tccagattct
    16021 caaatgatag gagatataaa aatagactta gcaatagtta ctcatgaaaa atctacacta
    16081 gaagcaaatg ttgcaaatat ggctaaagag tatttaacaa aaatacaaac ttataataaa
    16141 atgccatata atgctatgaa attgaatcaa tcagaaatag aagtagatta ttcatatagt
    16201 ttacttaagg aaactaataa ggaccttgtg ttaagtgttg taaaaaaggc tgaaaaacaa
    16261 gaggcattag tagttagatt ttacaattct aaagaagaaa caaaatcagc taacatagta
    16321 tttaacaaaa atattaaaac agctaaatgg attaacctaa atgaaaaaga attagaatct
    16381 atatcaatag ataataactt tgtaaatgta gagtgcttaa tgaaccaagt taaaactata
    16441 ttggttaaat agtgctaaaa aggaattgtc ttaaaataga gataatcctc cttatcattg
    16501 ttgaaataat aaacatatat ttgattttat acaaaaagag tgtctcatga actaagaaat
    16561 ttattttgag acactccctt tttataagat aaaagaatat taatggtata agttgtatac
    16621 aaagagagat gtagagtata aaataaaaga taaattaagc ttataaaatt atatgtttaa
    16681 aataaggagt aataaaaagg agtagataag tatgaaaata ttaatttcta tagattcttt
    16741 gaaaggaagc ttatcttcaa tagaggcagg aaatgcaata aaaaagggga tattgaaggt
    16801 taaagaagat gcgcaagtaa agatattgcc acttgctgat ggtggtgaag ggacagtaga
    16861 tgctttagtt caagggatga atggtaaaaa agagactata gaagttactg gaccaatagc
    16921 taaaaaagtg gatgcaacat atggattatt aaaaaataca agtacagcaa taatagaaat
    16981 agctcaagca tcaggactaa cattggttcc gactgaactt agaaatcctt tatacactac
    17041 aacttatggt gttggggaaa taataaaaga agcaataaat aaaggctata gaaattttat
    17101 agttgggata ggtggaagtg ctactaatga tgctgggata ggtatgcttc aagccttagg
    17161 gtttgagttt tatgatgaaa ataacaagtt agtaggatta ggtggaaaag ttttaaatga
    17221 aataagacat ataaaaatag aaaatagatt aaaggaatta gatgaatgta agtttaaaat
    17281 agcctgtgat gtaaataatc cgctgtttgg gaaaaatgga gctgcataca tatacgggtc
    17341 tcaaaaaggt gcaacatcag aaataattga ggagttagat aatgggctta gaaatttttc
    17401 caaagtagtt aaaagttact taagtaaaga tgtagctaat gtagaaggag caggagcagc
    17461 aggaggatta ggttttgctt ttttagcatt tttaaattca aaattagaat ctggaataaa
    17521 aataatatta gaagaaatta aattagagga agagttaaaa gatgcagatt ttgtcataac
    17581 tggtgaaggt agattggaca atcaaactgc tatgggcaag gctccaatag gagttgcaaa
    17641 actggcaaaa aaatatgggg taaaagtaat aggtttggct ggagccacaa cagaagatgc
    17701 agttaaatgt aatgaagagg gtatagatgc ttatttttct atagtaaata gagctatgac
    17761 aatagaagaa gcaatggata aagcaactgc tagtgagaat atgacatcta caacaacaca
    17821 aatattcaat cttataacta gtatacaaca gtcaaaaaat atgtagtatt aaaacaaggt
    17881 atattaggta aaaagctgtc acatattaat ttagactttt tagtgtgaca gcttatctat
    17941 tttatgttaa taacttaaga aacatatatt ggaaataaat taacggattt agatattttg
    18001 aataagataa gctattctat tcataaaact actatagtta tctgccttag taagttcttt
    18061 aacattattc cactcagata atatttcaat aaagctttca aatatgtaat tgaatttttc
    18121 tttttctttt tcatttatag gtatcatagc tataatctga actttttcct caccccattt
    18181 aactggttta tcgttaacaa ttaaacatac tccagttttt aaagcatcca ttttcatgct
    18241 atgaggaatt gctacattgt tattaaatgc agttgaagac attttttctc ttataagaac
    18301 atcatctata taattgggtt tgacatattt attttcgtac agaatatttc ccataaattt
    18361 tatataatct ttagcactat ctaaatatat atttttcata aacaaagatt cactaaagta
    18421 catttctaaa tgattttgta cagtaagtat atttttttta tctttaatct gaataaattt
    18481 actttggata ttatcataat cctttctatt taggtaagga tttacataaa caaaaggtat
    18541 agggcatcta tcgttaattg gtaaagtagt aataaccatg tctaccttat tgtcaaaatc
    18601 aagattatga gtatctgaaa aaatctgaat aatttctatg gattcattaa atctcttctc
    18661 gaggtctctt actattttga attgtaaatc atagtaattt ggagtaatta atgcacatag
    18721 aactttatct tctaataatt tttgtctatc aaagaatgaa cctacatgaa gagatatata
    18781 agatatttca tcttcgtcta tatctacatt ttctaaagtt tgaatctgat tgcaaatata
    18841 aacagatata tcatatatca aagggtagga atcttttaac ttttgaggta tttgatttct
    18901 taaaacttga ttatttttgg ctctactgat taaatttttt acatgaagag taaattttac
    18961 aacaaattcc tcatcagata aatctataag atataagtca tatacatttt taattatttt
    19021 tttagttaat ttaacaaagt gatcatctat ataatttggc aatgacttag tatccatttc
    19081 atgataattt aagactgtgg ttttgtttgt taactgaaat actaagtagt ataattctgc
    19141 actagttaaa gttatattgt attcagactc taaaaaattt gatatatctc ttgaaatgct
    19201 tatatcatta ttattatcta tgtagtttac tacttcgaca gattctatgt agttattctt
    19261 ttttattctg tctatagtta tcattaaatg aagaagtatg ttatttatag catactcatt
    19321 tataaacaaa ttgtattttt ttaatatata tattatattt tctttcagtt tggtcaaatt
    19381 atactcttga tatatttttt gtacatcaaa taagcttaaa aaatcacttc ccgtttcttt
    19441 aaatattata gagctcatta atttgcgctt tgcagactca tttccaatta gtttcaataa
    19501 aaatttcgat tgctttatct ttaaattata acttcctata aatttattag ctttgactat
    19561 atccccttca atagtagctg ggctaacaaa caattcttca cttaaatcaa atatattaat
    19621 tccattacta ttaactataa gttcttttaa aattaaattt aacctgttat caggtgatat
    19681 attttctgaa ctatcaacct tggtttgaca attattattg agatttactt tatatccttt
    19741 tttagaggat aatataaatt caccattgga attaatttcg tttacatatt ttctaattga
    19801 acgcgttgag acattaagat aattagccaa tgtagatgaa tctatccaat catgttgtgt
    19861 ttttatgaaa tttaaaaata gttctttttt agatgttttc attagattcc ctcgcatagt
    19921 tttaaaataa aatctggatg atatgataat tatataattg aatgtacgta ttataagata
    19981 cattatacta tattaatatg tttaattaag agtaactgat ttcctattat taggaaatat
    20041 atttatgtga aatgaactta tttggtaagt acaatataaa cataataaaa taaaaagttc
    20101 attaagaaat aaattaatgg ataaaaatat tggaggtatt attatgaaaa atataagagt
    20161 gcttttggcg tgtggttcag gagcaagtac aggttttatg gcaactagta tgagaaagag
    20221 tgcaaagaaa aaaggtatag aagcagatat aaaagcgaga agtgagtcag agatagataa
    20281 gtatttatca gaaatagatt gtttattact aggaccacat ttagattata tgaaggaaga
    20341 aattcaacaa aaggctaatg aatataacgt tccagtagca tgtatatcta aaactgcata
    20401 tggaagacta gatggtgcaa aggctttgga agaagcacta gaactaatat caaacaaata
    20461 aaatatttat ggggagagtg tttatgaata aatttataga ttttttaagt gaaaaattta
    20521 ctcctgttgt aaataatatg actaagaata tatgggtaca atcagttcaa tcaactataa
    20581 tgaaagtttt gccaatggta tttgtaggct cattagtaac tatagtttct gtcttgaaga
    20641 attacataag ctttttacct gatttaagtc caataaatca atatacattt ggacttttag
    20701 gattatttat agcattttta ttgcctatgg aaataatgaa gaataagaaa tttgaaagta
    20761 tgagtgtagt cgctggtctt gcaggtgctg gattaatgtt aatgatgatt cgtccagaga
    20821 ttacgaatga aggagctata tttaacttca acaggtttgg aggagaagga atgcttgtat
    20881 cgctagttgc aggattattc tctggattaa taatgtcctt atttggttct ttttcatttt
    20941 tcggagaaga ttcagcactt ccggattttg taagcaagtg gtttgataat atgttaccta
    21001 taacagtgat aatgtttgta ggctggggat taatttatgg actcaacttt gacatgtttg
    21061 aaataatagt tagagtattt aaaccagtat cagatatagc acagacatta cctggatttg
    21121 tactcttaaa ctttttagta gtatttatat attctttagg aataagtggg tgggtactat
    21181 cccctataac ttattcaatt caacttggtg caattgcagc taatgcagca gcagtagcat
    21241 caggaggagt agcaactaat atatatgttt acgaagttat tcaaacaggt tggataactt
    21301 taggagggtt gggtgctaca ttaccattag ttataatgat gtgtttatcc agagttggaa
    21361 gaataaaagc aataggaaga gcatgtattg tcccttctat aatgaatata aatgaaccag
    21421 taatatttgg agctccagtg gcatggaacc catttttaat gttacctatg tggataggta
    21481 caatagtaat atcaataact acttatttat caatgagtat gggatttgtt acaattccaa
    21541 gtaaggtgtt taatttatgg tatgttcctt ttggtatatc tacttttatg gtaaatcaag
    21601 atgttagagg tcttattgtt ttagcaataa atatagtatt gctatttatg atatggtatc
    21661 cattttttaa agcttatgag aaagaagaga taaaattaga gagccaagag gtttaaattt
    21721 agaaattgaa aggaaattat aaatatgaca attgaagaac atataataga tgaagaatta
    21781 gtagaggtgg caatgcaaat aatacttcgt gcaggtgagg ctagaacaga aattaaacat
    21841 gctcttaatg atttggaaaa gtttgattat agaaatgctg atttaaaact agctaaagca
    21901 aaagggttta tgacagaagc acatagagct caaaccaaga ttatacaagg ggaagctgat
    21961 ggagagaaaa gagcacattc gctgttattt gcacatgctc aagatactct tatgaccata
    22021 ttcagcgagt taaatataac tatgtcttta gtaggaatag taaagtctat agaatataag
    22081 gtaaataata gtaaatgatt taaataaata aaataaggag tgattaaatt gggtaagttt
    22141 cctaaaggtt ttttatgggg aggagctgta gcagctaatc aattagaagg tgcatggaat
    22201 gaaggtggca aaggttggtg tttagctgat gtacatatgt atgataaaga tagagatata
    22261 tctaaacctt atgagtctga tatgactata gaaagaataa agtttgctat gaaagataat
    22321 aatggatatt atcctaagcg tagtggaata gacttttatc atacatacaa agaagattta
    22381 aagcttttga aagagctagg gttaaagtgt tttagatttt caataaattg ggctagaata
    22441 tttccaaatg gagatgattt agagcctaat gaagagggat taaagtttta tgataatctt
    22501 ataaatgaag taatagataa tggtatggaa cctattgtaa cactacttca ctatgagact
    22561 cctttgaatt tagtattaaa atataaagga tggtctaata gaaaagtggt agatttcttt
    22621 gttagatatg cagaattaat actaaataga tataaaaata aagttaaata ttggatagtt
    22681 ataaatcaaa taaatttaat acattatgaa tcatttaatt cagttgcatt ttgtgtagac
    22741 caggtagatg atgtagaaga ggccaaatat caagctattc atcatcaatt catggctaca
    22801 gctttaacta agaagcttgg aaaagaaata aattcagata tgatgatagg gatgatgatg
    22861 gcagattgta catcttatcc agaagattgc gaccctgaaa atgtggtgtt tgctttaaaa
    22921 agaaatagaa tacaatattt tttcactgat gtaagtttta ggggagagta tccagtctat
    22981 attaagagat ttttcaaaga aaataacata aatatagtta tagaggaagg agatgataaa
    23041 atcttaaaag aaaatactat ggattttcta gctttatcat actattattc tacaacagta
    23101 tcagcaagta aaaacaccat gaatccaact gataatataa agaatccata tttaaaagca
    23161 aatccttggg ggtgggcaat tgacccaaaa ggattataca atactttatc tcaatattat
    23221 gatagatacc aaaagccaat aatgatagct gaaaatggat ttggaatgca tgataaattt
    23281 gaagatggaa aagttcatga tagttacaga atagattatt taggaaaaca tatagaacaa
    23341 ataggtgaag ctatagctga tggaaccgaa atattagctt attgtatgtg gtcgccaata
    23401 gatatagtaa gttgttcatc tcaagaaatg gaaaaaagat atggatttat atatgtggat
    23461 atagataaca atggaaatgg aagtaaagaa cgttataaaa aagatagttt ttattggtat
    23521 caaaatgtaa taagaactaa tggagaagaa ttaagagaaa agtaaatagt ttttaaatat
    23581 aaacaacccc ccttataaat actgtatata ttattaatat agttttataa ggggggttat
    23641 aattgtaaat aaaatgattt attaattata tgattcatag cgaaatttat atttatactt
    23701 ttaatcataa acatatcagt atgaggaata tctattgtac ttcatccaaa ttttggtaga
    23761 ggtacaattc ttttttattt taaaatgcta taagataaca taataaactt taagcaaaaa
    23821 attattatat tataaattta catatataga cattgactaa atcttaattg tatataaaaa
    23881 taagtagtta agaggtaata tattatatta ctcgataaat ttagacaaat aaaacttata
    23941 taaggggaga taacatatga taattgagct aaacaaaagt aattctgaaa atctaacgaa
    24001 gacggaactt gaaataatta aatttataaa tgaaaatgaa tcaatattac cagaattatc
    24061 tatagtagag attgctcatg aaacatattc atctccagca accgtttcaa gagcaattag
    24121 aaaatgcggt ttaaatggat tcaatgaact taggtataga ttaacagtaa aagatgaaaa
    24181 tgatgatata caaaatatgg gggatataat aaataaatcc tttatagaag cacagagagt
    24241 tatagaacaa atatctttaa ctacactaat caatataata aaaactataa ataaatctag
    24301 taagatatat attttagcaa gagggcttac tgaatatgta gcaaaagagt ttagtcttaa
    24361 attgcaatta cttgatttta atgttgtttt tataaatgac cccaacataa tgaaaataaa
    24421 atctaaatca ataaaaaaag atgaacttat aattatattt tcattgaatg gaaataccat
    24481 agagcttgta gaatcagcac agaatgccaa tttagttgga gctaaggtta taacttgctg
    24541 ttgtagtgaa agtgcaaaac ttataaagta ttcggattta tatattgtag gatacaaaca
    24601 tgaacatata tcaatttctg aatatgaagt atcttccaga ttgcccttgt atattatatc
    24661 taggataatt atagattata tggtcagata taataattct gaaaattaaa ttgaaaagtc
    24721 tatgaaaatc gattttctat ttgtgaaaag attttcatag acttttttat tataatgaaa
    24781 acatacacag cacaagcaag ggggaagaca atgatatata cattaactac aaatcctgca
    24841 atagatatga atatatctac taatgggata aagagaaaat tagtaaatcg tacatcaaat
    24901 gctgtatatt ctcctaatgg aaagggatta aatgtaactt ttgtactagg tcattatggt
    24961 atagaatcta agatattagg attttttgga ggattttcag gaaaatatat agttgaagaa
    25021 agcgaaaaga gaggatttga cgtattgcct acttgggtag aagatacaac tcgtataaat
    25081 atatttttaa atgatggaag tgatgaattt aaatttgtaa actctgggtc atatgtaaat
    25141 gaaaaacaaa aactagatat gctagataag atacagtcat tagcagatat aagttactta
    25201 tcaataagtg gaagtctacc tcctggtatt gatgataatt actatgagga tatatttaaa
    25261 atttgtaaaa ataaaagtat aaagacaata ctcgatataa gttcaccaaa attaaaggag
    25321 ttactacagt ataatccata tttgataaaa ccaaatgatg aagaaatcaa agatatattt
    25381 ggaattattg taagagatga agaagatatt aaggatgtac ttaaattact acatttaaaa
    25441 ggtgctcaaa atatattgct aactttagga gaaaaaggtt cttattttta taatggaaaa
    25501 tcaatttatt atgctagtgc tcaaccagtt acagtagtca gctctgcatg tgctggtgat
    25561 tctgcattag ctgctttttt aagtatatgg ttggaaaatc cagaacatat tgaagaagca
    25621 ttaaaaaggt cagcagcaac aggagcaagt gtggctgaaa gtaatggtat aggtaatttg
    25681 gagaatgtgg aagattatat aaaaaatata agagtcagaa aggtggtata aaaatgggaa
    25741 agaaaatgat tctaggggta acaggttgtc caactggaat agctcatact tttatggcag
    25801 aagaagcatt aaaaaattca gccagggaat tagggtgtga cataaaagtt gaaacgaatg
    25861 gagctatagg tgtagaaaat aagttaacag caaaagatat cgaaatggca gatgcaataa
    25921 tagttgcttg tgataaaaat gttgatatgg acagattcaa tggaaagcca gtaatagaag
    25981 tacctgtcaa agaaggtata cataaagcta atgaacttat acaaagatgt attgatggaa
    26041 aagtagctat tagaaaagga aacttatcaa gcagtaaaat tgcagaagaa ggaaacttat
    26101 cgtttggtca aaagttgtat aaagacttaa tgaatggtgt atctcacatg ttacctttag
    26161 tagtagcagg tggagtactt actgctatat catttttatg ggggatttat tcatttgacc
    26221 caaattcaga acaatataat caaatagcag ctacattaaa atctgttggt ggatattcta
    26281 tgaacttgat ggttcctgta ttggcagcat ttatagctca atctatatcg ggaagagctg
    26341 gtatgttggc aggacttgtt ggaggaatga tatcatttga tacaggttcg gggtttttag
    26401 gtggaatcat atcaggattt ttagcaggtt acttagttaa actatttgta catttgttaa
    26461 gaaaattacc acgacaatta gaaggtctta aatcgatttt tataattcct attgtcagcg
    26521 taggtcttgt gggtgtagca atgttgttac ttggtgggcc atgttcagca cttaacaatg
    26581 ctatgatgaa ctttctatcg gggttacaaa attcaagccc tataattttg ggaatagtaa
    26641 taggatgtat gtcagcattt gatatgggag gtccagtaaa taaagcagca tatgtaactg
    26701 gaactatgct tctaggacag ggaaattatt tatttatggc aggtgtatct gcagcttgta
    26761 taacaccacc attggtaata gcaatagcat ctactttatt taaaaataga tttacagaag
    26821 aagatagagc agcaggatta atcaattata tactaggtag tactcatata acagaggggg
    26881 caataccttt tgcagcaaaa aatccattaa aagtgttgcc agtacttatg attgcatcat
    26941 caatatcatc aattttaact tatataatga aaatagaagt accagctcca catggagggt
    27001 ttttggttct aggattagtc aataagccac ttttatgggt agggtgtata tttgtaggtt
    27061 cattagtagg tgctgtactt tatatgatgg ttacacctaa agtagtaaat gagaaaaata
    27121 ctatagaaaa tacaaatgca acaaaagatg aacataaaaa tgaaagtgca gaatcggaaa
    27181 acgtatctaa taatatctct ttatatagcg aagaaacagt aattttagat gttaagggaa
    27241 aaaataaaac agatgtaatt gatgaaatga ttggaatact tgataaatct ggtgttcttt
    27301 tagataaaaa gaagtttaaa gaagaaatcc ttaaaagaga agaaatatca tctacaggtt
    27361 ttggtatggg gatagcgata ccacatgcaa aaacagatgc agtaaaggta ccacgtgtag
    27421 cagtaggtgt atcaaaagaa gggtttgact ttgaatcaga agatggaagc ttggcacata
    27481 ttatatttat gatagcagct acagataatg gtgataattt acacttaaaa acattatctc
    27541 aactatcttc aaaattaatg aatgaagaat ttttaaatga gttattaaat agcaaaacaa
    27601 gtagagaaat agtatcaaaa ttaaataacg aagaaattaa atcagtataa acatatagga
    27661 ggagaaatta tggcattagt gacaactaaa caaatattat tagatgcaca agaagggcat
    27721 tatgcagtag gtgcatttaa tgttgaaaat atggagatgg ttatggcagt aatagaggct
    27781 gctgaagaac ttaaatcacc agtaatattg caaacaacac cttcaacaat aaagtatgca
    27841 ggactagatt attatttagc gaatgtaaag gtagcatcag agaaggcaag tgttcctgtg
    27901 gcaatgcact tagaccatgg ttcaagcttt ggactagcta tgcaggcact tagagttgga
    27961 tatacttcta ttatgattga tggttctcat gaaagttatg aagataatat ttcaatatct
    28021 aaatctgttg tagatgcatg tatgccttca gatatacctg ttgaagctga attaggtaaa
    28081 gttggaggta aggaagacga tttagatggt ggtgatgggg gagcatatac agacccatta
    28141 gaagcaaaag agtttgtaga gagaacaggg gtatcatctt tagcagtagc aataggtacg
    28201 gcacatggat tatataaggg agagccaaag cttgatttag atagattatc tgaaattaga
    28261 gatgttgtat cagttccatt agtattacat ggtgggtctg gaataccaga tgaagttatt
    28321 aaggaatcta taaaaagagg gatttgcaag gttaattatg caactgaatt aaggattgct
    28381 tatagcaatg gagttaagaa tgtgcttaat tctgacccag aggtaattga ccctaagaaa
    28441 tatggtaaaa aaggtttaga gtcagttaaa gattttgtta agagtaggat ggaagtttgt
    28501 ggatgtgtag gaaaagcaat agagacagtg aagtgttaat aataaaggga aagaataagt
    28561 aagattaaat taaggggtgt ctcaaaatga actttttagt tcatgaggca ctcttttttg
    28621 tatgaaatta aatatatttt catcattctg acaataaaaa agaagattat atctattttg
    28681 agacagcatc ttattttatt ttttgattaa tattgtaatt gagaaatttg aaataaggaa
    28741 gatgatataa ttaaataaaa aagttctttc taagtttaaa ttaatattaa aaagttataa
    28801 tatggtaaga attatccagg tagatgactt ctatgatgga tatatgataa aataattttg
    28861 tatggtatat ataataatca taaaataaga gtattgcatt agttttttaa ctaaaacatg
    28921 taaataggaa aaataattta ataactgtgt agtaaaaact tggatttaac ataagacact
    28981 aactattaag gcgtacgact aaaatatata tttaaattat tttatgaaca ctggagggaa
    29041 agtcatatat gattacagga gaattaagga gcaaagtaga caagatttgg gagacatttt
    29101 ggactggtgg attaactaat ccattagaag ttatagaaca atttacatat cttttattta
    29161 ttaaaggatt ggatgaagta gaaacaaaaa atgaagcaga agcaactctt ttgggagttt
    29221 cttttgaaag gatatttaat gatgaacatc aacatttaag atggagtaag ttttctaatg
    29281 aaggaaattc agaaaaaatg tatgaaatag tacaaaatga agtgtttcct ttcataaaaa
    29341 aattacatgg aaataaagac tcagcttatg caaagtacat gggagatgca atatttaaaa
    29401 ttcctacgcc tcttatgtta tctaaaatag ttgatggtat tagtaattta gaattaagta
    29461 aggatagaga tacaaaggga gatttgtatg agtatttatt atccaaagtg gctacagcag
    29521 gtactaatgg tcaatttagg actccaagac acataataga tatgatagta agacttatta
    29581 aaccaacacc agaagatatt atagtagacc cggcagcagg ttcagcaggt tttttagtat
    29641 catcacaaca atacttaagg gataatcatt caagtctttt cttagtgcaa gggctgaagg
    29701 agcattttaa taatggtatg ttctatggat ttgatatgga tagaactatg cttagaatag
    29761 gtgctatgaa tatgatgttg catggcgttg acaatcctaa tatagagtat aaggattctt
    29821 tatcggaggt taatacagat aaagataagt ttacattggt gttagcaaat cctcctttta
    29881 agggaagttt agattatgaa gcagtgtcag cagatttatt aaaagtgact aagacaaaga
    29941 aaacagagtt attattcttg gcactttttt taagagtttt aaaaattggt ggaagatgcg
    30001 catgtatagt gccagatgga gtattgtttg gttcaacagg tggacataaa tcgataagaa
    30061 aagaaatagt tgataatcat aaattggaag ctataatatc aatgccaagt ggagtgttta
    30121 agccttatgc aggagtatct acagctataa tgatatttac taaaacagga acaggtggaa
    30181 ctgataaggt atggttctat gatatgaaag ctgatggtta ttctttggat gataagagaa
    30241 atgagataaa ggatagtgat atagaggata ttattaagag atttgataat cttgatggtg
    30301 aagtagatag aaaaagaaca gagcaaagtt tttttgttga tgtggaagag ataagagaga
    30361 atgggtatga tttatctatt aataagtata aagaggtagt gtatgaagaa gtagtttatg
    30421 atgctcctag tgttattatg ggtagggtta aggcgttaga gaaagagata gtggatggat
    30481 tagaggaact agaaagaatg ttagaggtgt agagtatggt tgatgaagca aaaattttag
    30541 atgttgttag tataagcggg gaaaatgtaa aaaagtttga tggagaacgc tcatatctta
    30601 gtactggaaa cttagatttt aacaagataa gcaatctaga aatagttact tatgaaaata
    30661 aaccttctag agcaaatcaa actgtaaata taggagaagt tatatttgca aaaatgaagg
    30721 acacaaagaa aacattagtt attaataaaa ctaataaaaa tataatagta tcgactggat
    30781 tttatgtttt aaaaccatca aaagaaatat tgcctcaata tttataccat tacttaaata
    30841 gtagttattt tctaaatcaa aaaaataggt taagtaaagg tgctacacaa tcagctttaa
    30901 ataatgaagg attagctaat ataaaaatac gcatgtataa tttaaaagtg caagaaaagg
    30961 tagtgcgtgt tttagacaag gctcaagagt taatagataa aagaaaagag caaatagaag
    31021 tattggatga attagttaaa tcaagattta ttgagatgtt tggtactcca tcaaagaatg
    31081 aaaaaaactg ggaaatttca gaaataggga aatatttaga tgttttaact gattatcatt
    31141 caaatggtag ttatgaaacc ttaagagata atgtaactct tttagataca aaaggttatg
    31201 ccttaatggt aagaactaca gatttagaaa ataataactt tgaaaaaggt gttaagtata
    31261 tcgatgaaca tgcatataat tatcttgaaa aatcaaaggt gtttggtggt gaggttataa
    31321 taaataagat aggtagtgca ggtaaagttt atttaatgcc atttttaaat aaaccagtat
    31381 ctttagctat gaatcaattt atgcttagat ttaatgaaga taaagtgaat catatattct
    31441 tatataacct tttactaaca tcctatatgg aaagtaaaat aaaagagaaa gtaagaggag
    31501 ctgtaacaaa aacgataact aaagatgctg ttagaaaaat aaatattatt gtacctccta
    31561 ttagacttca aaatcaattt gccaacttcg ttaaacaagt aaactctttg aagtttgaaa
    31621 tggaaactag cttaaaagaa ctagaagaca actttaactc attaatgcaa aaagctttta
    31681 aaggagagtt attcaactaa aatacaatta ttaaaatggg ggaataaaat ctatgaatca
    31741 aatagagcta gatttatacc ataacatggt aaatatctat aaagaagctg atttacaatg
    31801 taactataag ccaacaagat ttttacaact gataaataac aaaggagcct tattagctgc
    31861 taaagaatta atcaataaag aaggagctac tgaaggattt acaagactat gggagtgtaa
    31921 gagattagat ttatcattag aagtattagt attaaaagaa aagtataaag agttgtttac
    31981 tgatgaagaa agagaaatat gtataaatag attaaaagat tatggatata aatttaatta
    32041 agtaatatta cctaataaac aagaggtgat taaatgagaa atataccaaa tgagattaaa
    32101 aactcaataa taaatgtatt tccaaaagaa aatactctag caatcataat atttggtagt
    32161 tatggaacag ataaacatac atcagaaagt gatatagaca tagcttggat tcctatcaaa
    32221 aaagtaccaa ttacagaact tgcaactaag acagaagctc taagaaatat attagacatt
    32281 gatgtagacc taaaaattgt tacagataat tatacaatag cacttaaaaa agctatttta
    32341 gaaggtgaag ttatttatga atctaaagat tttaaggatt atataaataa attctatttt
    32401 gaaaacagtg atgtaataga tatcttagat tggagggaca tgtatggtaa ttagaaacaa
    32461 attatcttgg aactcaatag aagaagtaaa tagggatttt ggttcttgct tgtatgacct
    32521 gcaaaacttc aaaatcttat atgataagga agaaatgcct gagttatggg ctagatacat
    32581 aaaagaaggc tttaaaagct atatggtatc atttctagaa ttaacaaaag caatgttgta
    32641 ttataaatca attgatttaa acataaaatc taaaaacttt tatgactacc ttttagcttg
    32701 tgagtatcac aatttattac ctaaaaattc ttctatagtt atagaaacac ttagaaaatt
    32761 gagaaatgat gattctcatg gatatgatat accacagttt gaagatatgt acgaattgtt
    32821 tacagaaaat gaagaagtat ttgttagtat aagaaacagc tgtaagaaat agtttttaga
    32881 gagatttatt ttaactaagg gacaaaaggg ggataaaagc ttgggtaatt ttgatttttt
    32941 acaaaagaag aaaaatttca atagttttaa tactgcttgc ttagaagcag aaaaaagtat
    33001 actagttagt ccatctactt gtgcaactat aacaagaaga gctttagagc ttgcagtaaa
    33061 atggctatat gctaatgata gtgatttagt attgccttat caagataatc tcagtacgtt
    33121 gattcataat aatagtttta tagaacttat agactatgat atgttacctt tattaaaata
    33181 tatagttaaa ttaggtaatt tatctgtaca tactagtgcg aacatagaga gaggagaagc
    33241 aatactttca cttaacaatt tgcatcaatt tgttagttgg atagactatt gttattcaga
    33301 tgaatataca gcagaagact ttaatgaaga tttattactt tatggggaag agaaaaggac
    33361 gagaccggac gaacttaaaa atctttatga gagattaagt tcaaaagata aaaagcttga
    33421 agaagtaata aaagaaaatg aggaattaag aaagtcacta actgaaaaga gaaaagttaa
    33481 tactgtaaac tatgatttca agatagacga agtaagcgaa tttgagacaa gaaaaagata
    33541 tataaatata gaattaaagc ttgcaggttg ggaatttgga aaagatatag gggaggaaat
    33601 agaagtacaa ggtatgccta atgaaagtgg tgtaggatat gtagattatg ttctttatgg
    33661 tgaaaatggt aagccattag cagtagttga ggcgaaaaga acgagtaaag atccaaaaat
    33721 tggtcaacaa caagctaaat tatatgcaga ttgtttggaa aagagatata gtcaaagacc
    33781 agtaatattt ttgactaatg gttttgatat gtatatttgg gatgactatt cagatagaaa
    33841 agtttatgga ttttataaaa aatcagaatt acaacttatg atagatagaa gaaagagtaa
    33901 gaagtcttta agttctgtaa caataaatga tgaaatatcc aatagatatt atcaaaaaga
    33961 agctattagg gctgtatgtg aggctcttga aaataagcaa cgcaaaactc ttttggtatg
    34021 tgctactggt acaggaaaaa caagaattgc tatatctata gttgatgtac tttcaagaca
    34081 taattggatt aaaaatatat tatttctagc agatagaaaa gcattggtaa agcaagctaa
    34141 aaaaagtttt actaagctat tacctaactt ggcgttatgt aatttattgg atagcaaaga
    34201 cagcccagaa gatgctagga tgatattttc tacatatcca acaatgatga atgcaattga
    34261 tgatacgaag tctaaggatg gaaagagatt gtttacacct ggccattttg atttaattat
    34321 aatagatgaa tcgcatagaa gtatttataa aaaatataag tcaatctttg attattttga
    34381 ttcttactta atgggtctta ctgctacacc aaaggatgag atagataaaa atacttattc
    34441 agtttttgac atggaaaatg gagtacctac atatgcttat gagtataata aggcagtcga
    34501 ggatggatat ttagtagatt atactagtat tgagtttaag actaagatta tggaagatgg
    34561 aattaagtat gatgagcttt ctgatgaaga gaaggaagaa tatgaaaata catttaatga
    34621 tgatgagagt attggagacg aaatagggaa taatgcagta aatgagtggc tatttaattc
    34681 tgacacaata gatttagttt taaataagct aatgacagag gggttgaaaa tagaaggaga
    34741 agaaaagatt ggtaagacta taatttttgc aaagaataca aagcatgcta gggcaatagt
    34801 tgaaagattt aataagctgt atccaaagta tggtgggaac tttgtaaaag cagttgacta
    34861 tagtataaat tatgtagatt ctataataga tgatttttct gataagaata aattacctca
    34921 aatagcagtt tcagtagaca tgcttgatac aggaattgac atacctgaaa tactaaatct
    34981 tgtttttttc aagaaagtga gaagtaagac taagttttgg caaatgatag gtagaggaac
    35041 tagattatgt ccagatttat taggtgttga tatggataag gagagattct taatatttga
    35101 tttttgtaat aattttgaat tttttaaatt taatcctaaa ggatttgagg gaaataaagc
    35161 agaaacactt actgaaaaat tatttaatat aaaggtaagt atggttaaag aacttcaaga
    35221 tattaaatat atagaaggtg agtatgcaga acttagaaaa gaattgttag aagaacttat
    35281 aacttctgta aaagctttaa atgaggatag ttatatagtt aggatgaatt taagttatgt
    35341 acataagtat aaaaatgaaa atgtttggag caatattgga gcagtagcac aaaatgagat
    35401 aagagaatat atatcaccac tcataacttc atatagtgat gatgaattgg ctaaaagatt
    35461 tgatgtagtt atgtataata tacaacttgc atacttacaa aataataatg caagtaaagg
    35521 tataaggcat gtaatggcta cagcagaaaa actttcaaaa cttgggacta taccacagat
    35581 tcagcaacaa aaatatacta ttgaaaaagc aatgacagag gatttttggg aagattcaga
    35641 tatatttgag gtagaagaag tacgtatatc attaagagag cttattaagt atcttgaaaa
    35701 gtctagtcaa aagatatatt atacaagttt tgaagatatg attgtagcag aatatagaaa
    35761 tgattctgta tataatgcaa acaatcttaa gaattataaa aagaaggtag agtattattt
    35821 aaatagccat aaagatgaat tagcaatatt taaattaaga aataataaaa agataacaaa
    35881 gcaagatgtt gaaacgttgg aggaaatact cttaaaacag ttaggtaatt gtgatgatta
    35941 taagaaggag tttggagata ctccagtaag ccaattagtg agaagactag tgggattgga
    36001 tagagaagct gcaaatgaag ctttttcaga gtttttaaat aataagagct ttaatactaa
    36061 gcaaatacat tttgttaaac ttatagtcga ttatgtagtc aagaatggat ttatagagga
    36121 taacaaagtg ttgatggaag acccatttag aactgttgga agtataattg atttatttga
    36181 aaatcatatt gaagagagaa ataaattgat taaaactata aataaaatta aagaaaatgc
    36241 atcagagata ggctaatgat gttatttagt gcaaaattat tgttaatgat gtagagaata
    36301 aatttattag taggtagtct aatattgggt tgaaatagat aactagatat ggataatgta
    36361 aatatacaaa gagaggtatt gaataattat gcaatatctc tttttaattg ataaataact
    36421 atgaattact catgaagaag attaaataat aatttttaaa attaagtatt tgtatatttt
    36481 atagaataat catgtattta ctttaataaa ataaaaacag gtataaaaag gtatataata
    36541 aggctttcat atacatttat gagtcaaaat tttgatataa tatatagata catggagtta
    36601 tgaatggaat ttatttaact tataggggga gttaatatga aagcaggaga aattgaattt
    36661 cttagttatt tagaggggtc aaacaaaagt tttgtagtac ctgtatatca aagaaattat
    36721 aactggaaga aagaacaatg taaaagattg tttgatgatt tagaggatat aatagattct
    36781 ggatttagaa ctcatttctt agggtcagtt gtatctatat atagtatggg aaaagaatat
    36841 ttaataatag atggacaaca aagagtgact actgtgtcaa tattattgat tgccatgtgt
    36901 caaataataa aagagtttgg ggaatcaaaa ggaattatta caacagaaga acagattaag
    36961 gagtgttatc ttataaacaa gcactcaaga gataaagatg gaaaaataaa gttaaaacca
    37021 atagcagaag atagaaaagc gtactatgaa ctatttgaag aagaaatgaa tacagataga
    37081 aattctaata tagtttcaaa ttatttatat ttttataata gaataaaaga agataatata
    37141 tcaatagatg atttgtttga tgctatacag aaattaacta tagtagaaat agagttaaaa
    37201 aatggtgaag atgaccctca attaatcttt gaaagtctaa attcaactgg cttagattta
    37261 agtgaagctg atagagtaag aaactttgtg ttaatgggta gaagctctaa gttacaagag
    37321 gaattttata ataattattg gtataaaatt gaaaaaaata caagtagcaa cgtaagtagt
    37381 tttataagag actatttgac tataaaggag agaagtatac cgaataaaaa taaagtatat
    37441 tttagtttta aaaggtatgt atcagataat ggtatagata tagaaatttt attaaaagat
    37501 ttacttaaat tttctaacta ttatagagag atatcttatt catgtactga tgataatgat
    37561 gtaaacaaag ttttgaaaag gataaacaaa cttgaaatat ttgtatcata tccgtttata
    37621 ttagaattat ttgatgatta taatgaaggt ataataaata aaaaagtact tgtagaatcc
    37681 cttaatttag tagaatgttt tatattaaga agaatagtat gtgatgtacc tccgaatgca
    37741 ctgaataaag tatttatgaa cttaggaagg gaaattaaaa aatttagtga ttataaagaa
    37801 aactattcta atatattaag atatattttt ataaataaga aatcatctca aagatttcct
    37861 actgacagtg aattttcaaa tgcttttgaa actaaagata tatataatac taagtcaaag
    37921 aataagttat atatacttga gaggttagag aattataata ataaagaaat tgttgattta
    37981 gaaaatttaa tcaataataa tatgctaaat atagagcata taatgccaca aacattaaca
    38041 aataaatgga aggaatcact aggagataac tataaggaga tacatgataa atatttacat
    38101 acaattggaa atttaacttt gacaggatat aattcaaaac taagtaataa aacatttgaa
    38161 gaaaagaaag aaatggaaga tggatttaaa gatagtaggc tttatctaaa taaatatatt
    38221 tcatctatag ataaatggaa tgaagaagaa ataaaaaata gaactaagat attactagat
    38281 agagctgtag aaatttggaa atatccaagt gttacatatg aacctccaag taacagtgaa
    38341 aatatattta cgttagagga tgatgatgtt aattttacaa atgaaagtat agtatcattt
    38401 agtatattag gagagtcata taaggctaaa aattggacag aattttatga acaagttgct
    38461 cttactttat atgatttgga ttctgtaagg tttaacagta ttgtagacaa aacttataaa
    38521 agagatttta tagataatat taagtctaga gatgcatata aattaagaaa aggtattaaa
    38581 atatcagaaa atatatattt agaaacaaat ttaaatacag aggctaaatt aaatataatt
    38641 agatttttac ttaatgagtt tgatatagag ttaaatgaag taatattttc cataaaataa
    38701 tgaaatatat gcattttttg cgtaatagaa tattaattat attaaaaata aaatagcacc
    38761 tcttgatata gatttatagc tcaaatttat atcaagaggt tacgtttaat taaagtaata
    38821 caaatactat ctaattaaat tattaataaa aatgttatat aaaaatgagt atatagcatt
    38881 ttaaagagta ataaacctta cataataaat gatttattaa tatgaactag aagtattttt
    38941 actatattgt tttataaaac ctttaggcat tttacttata aaatatacca ccataataca
    39001 agacaaaggt ttgtctacta tattactaag aatccttgga aagaacgcag cagtaaatat
    39061 cttctgacca gactgaacta aaaaaccaac caataaatca gcaccacttc cacttaaacc
    39121 accaaacaat aaaactgaaa taggagtacc aattaaagga caaacaatag ataaaattac
    39181 tcctgtaata atagcttctt tataaccaaa accttttttc ttagaaatat atccaacaat
    39241 aagaccaaga acaatattta caagggcaaa aggtatattt gtaggacctg atataacacc
    39301 taaaactaag tttgtacatc caccaataag agcaccccaa aaaggaccta atacgacagc
    39361 cccaagtaca gtaccaactg tatctaaaaa taataatggt atatttatca tagatataac
    39421 catacctaag acaatattaa taactatgca cattgaagaa aatgtgactt ttttagtatc
    39481 caattataat acctcctaaa aaaatatata catttatcat agtatagatt
//
LOCUS       DAENCV010000031        38297 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAENCV010000031 DAENCV010000000
VERSION     DAENCV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.031"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(205..618)
                     /locus_tag="KQD82_001490"
     CDS             complement(205..618)
                     /locus_tag="KQD82_001490"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG4379966.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(659..1789)
                     /gene="rodA"
                     /locus_tag="KQD82_001491"
     CDS             complement(659..1789)
                     /gene="rodA"
                     /locus_tag="KQD82_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG4379967.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1874..2158)
                     /gene="minE"
                     /locus_tag="KQD82_001492"
     CDS             complement(1874..2158)
                     /gene="minE"
                     /locus_tag="KQD82_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG4379968.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2176..2973)
                     /gene="minD"
                     /locus_tag="KQD82_001493"
     CDS             complement(2176..2973)
                     /gene="minD"
                     /locus_tag="KQD82_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG4379969.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(2997..3680)
                     /locus_tag="KQD82_001494"
     CDS             complement(2997..3680)
                     /locus_tag="KQD82_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG4379970.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3778..6756)
                     /locus_tag="KQD82_001495"
     CDS             complement(3778..6756)
                     /locus_tag="KQD82_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG4379971.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVVRQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6768..7256)
                     /gene="mreD"
                     /locus_tag="KQD82_001496"
     CDS             complement(6768..7256)
                     /gene="mreD"
                     /locus_tag="KQD82_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG4379972.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7271..8164)
                     /gene="mreC"
                     /locus_tag="KQD82_001497"
     CDS             complement(7271..8164)
                     /gene="mreC"
                     /locus_tag="KQD82_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG4379973.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8174..9184)
                     /locus_tag="KQD82_001498"
     CDS             complement(8174..9184)
                     /locus_tag="KQD82_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG4379974.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9305..9964)
                     /gene="radC"
                     /locus_tag="KQD82_001499"
     CDS             complement(9305..9964)
                     /gene="radC"
                     /locus_tag="KQD82_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG4379975.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10009..10593)
                     /gene="maf"
                     /locus_tag="KQD82_001500"
     CDS             complement(10009..10593)
                     /gene="maf"
                     /locus_tag="KQD82_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG4379976.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10769..11740)
                     /locus_tag="KQD82_001501"
     CDS             complement(10769..11740)
                     /locus_tag="KQD82_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG4379977.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11756..12331)
                     /gene="rsxA"
                     /locus_tag="KQD82_001502"
     CDS             complement(11756..12331)
                     /gene="rsxA"
                     /locus_tag="KQD82_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG4379978.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12346..12939)
                     /locus_tag="KQD82_001503"
     CDS             complement(12346..12939)
                     /locus_tag="KQD82_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG4379979.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(12952..13521)
                     /locus_tag="KQD82_001504"
     CDS             complement(12952..13521)
                     /locus_tag="KQD82_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG4379980.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13521..14498)
                     /locus_tag="KQD82_001505"
     CDS             complement(13521..14498)
                     /locus_tag="KQD82_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG4379981.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14521..15858)
                     /gene="rsxC"
                     /locus_tag="KQD82_001506"
     CDS             complement(14521..15858)
                     /gene="rsxC"
                     /locus_tag="KQD82_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG4379982.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16074..17018)
                     /locus_tag="KQD82_001507"
     CDS             complement(16074..17018)
                     /locus_tag="KQD82_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379983.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17129..18403)
                     /locus_tag="KQD82_001508"
     CDS             complement(17129..18403)
                     /locus_tag="KQD82_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG4379984.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18535..18906)
                     /locus_tag="KQD82_001509"
     CDS             complement(18535..18906)
                     /locus_tag="KQD82_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG4379985.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19134..19733)
                     /locus_tag="KQD82_001510"
     CDS             complement(19134..19733)
                     /locus_tag="KQD82_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379986.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(19934..20146)
                     /locus_tag="KQD82_001511"
     CDS             complement(19934..20146)
                     /locus_tag="KQD82_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG4379987.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(20219..21934)
                     /locus_tag="KQD82_001512"
     CDS             complement(20219..21934)
                     /locus_tag="KQD82_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4379988.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(21934..22488)
                     /locus_tag="KQD82_001513"
     CDS             complement(21934..22488)
                     /locus_tag="KQD82_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG4379989.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22481..23083)
                     /locus_tag="KQD82_001514"
     CDS             complement(22481..23083)
                     /locus_tag="KQD82_001514"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG4379990.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKIFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23092..25740)
                     /gene="polA"
                     /locus_tag="KQD82_001515"
     CDS             complement(23092..25740)
                     /gene="polA"
                     /locus_tag="KQD82_001515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG4379991.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            25892..25979
                     /locus_tag="KQD82_001516"
     tRNA            25892..25979
                     /locus_tag="KQD82_001516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25926..25928,aa:Leu,seq:caa)
     gene            complement(26166..26609)
                     /locus_tag="KQD82_001517"
     CDS             complement(26166..26609)
                     /locus_tag="KQD82_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379992.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(26612..26809)
                     /locus_tag="KQD82_001518"
     CDS             complement(26612..26809)
                     /locus_tag="KQD82_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4379993.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(27001..27867)
                     /locus_tag="KQD82_001519"
     CDS             complement(27001..27867)
                     /locus_tag="KQD82_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4379994.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPN"
     gene            complement(28093..28962)
                     /locus_tag="KQD82_001520"
     CDS             complement(28093..28962)
                     /locus_tag="KQD82_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4379995.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(29468..29689)
                     /locus_tag="KQD82_001521"
     CDS             complement(29468..29689)
                     /locus_tag="KQD82_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379996.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(30190..31170)
                     /locus_tag="KQD82_001522"
     CDS             complement(30190..31170)
                     /locus_tag="KQD82_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4379997.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(31334..32206)
                     /locus_tag="KQD82_001523"
     CDS             complement(31334..32206)
                     /locus_tag="KQD82_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4379998.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(32370..32921)
                     /locus_tag="KQD82_001524"
     CDS             complement(32370..32921)
                     /locus_tag="KQD82_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4379999.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(32941..33843)
                     /locus_tag="KQD82_001525"
     CDS             complement(32941..33843)
                     /locus_tag="KQD82_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG4380000.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(33858..36230)
                     /locus_tag="KQD82_001526"
     CDS             complement(33858..36230)
                     /locus_tag="KQD82_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG4380001.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(36451..37140)
                     /locus_tag="KQD82_001527"
     CDS             complement(36451..37140)
                     /locus_tag="KQD82_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380002.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(37383..>38297)
                     /locus_tag="KQD82_001528"
     CDS             complement(37383..>38297)
                     /locus_tag="KQD82_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4380003.1"
                     /translation="FFRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGS
                     LHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSI
                     QESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDI
                     IINEKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNK
                     YIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNG
                     ELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT        11808 a         7236 c         4014 g        15239 t
ORIGIN      
        1 tatttcatat tattaaaata gagtaatagt taaattctac tactctatct ataatctatt
       61 tcctaattat ttaatctcaa atttagctct tcatcattta aatctttcat aagatttcta
      121 caggtactag gtttattcca acagaaagac tagggcatta aacacttata tatctgtttc
      181 tttagactgt aatattaata ttttttaaat acgttgactt ttgctttttc taacttctct
      241 ccatgatagt tctcctctat caagagcttt tataaatatt tctgctgaag ctagatttgt
      301 agctattgga acatgataaa catcacaaag tcttattaac gcttgtatat ctgtttcatg
      361 agcctgagat gtcaatgggt ctcttaaaaa aataactaaa tctatttctt gtgttactat
      421 taaagaacct atttgttggt ctccacctaa aggaccagat gctaatctat ttatatttaa
      481 ttcagtttca tccattattc tttttcctgt agttccagtt gcatatagtc catacttttt
      541 taaaattgat tcataaccta tacaaaatcc gaccatagtg tttttcattt ggtcatgtgc
      601 tactaatgct atattcatat atctaatctc ctcaatttat gtttagatgt atttaaattt
      661 agaaatttat tttctttctt ctcatacaca catttagtac taatcctaag tttgctaaag
      721 atgtcaataa cgaacttcct ccataactta caaaaggtaa tgttactcca gttactggta
      781 ttaacgcaac cgtcatacct atattttgga ttatctggta accaaacatt cccataacac
      841 caaccactat gagggttcca taaaaatctt ttgcatctct tgcaattgct aaaagcctta
      901 ataggaatat cataaataag attatcaaca ctgccattcc tattacacca agttcttcac
      961 ctacaacagc aaatataaaa tcactatctt gtacaggtaa aaaatcttct tgattttgac
     1021 ttccattgta aagtccttta ccagttacgc ctccagaacc aattgcaatc agagattgca
     1081 taacttgata gtttcctttt agagttacat cttctggatt tagaaaagct tctattctta
     1141 ccttttgatg gtctgccatc attaaataca tcagtggcat tgacactaat agtatgatta
     1201 ttcctctttt tatcaacttt gagctaagac ctgctgtgaa cagcattgca aatatcatac
     1261 aacaaaatac aattcctgtt cccaagtctg gttgtgcaat caataatccg ataaatggaa
     1321 gagaataaac tacaacaggc ataacttcct ttaacgtatt taatttatcc tttttggatt
     1381 caagtatctt tgcataactt agtacaaatg ttaatttgac tatttcagat gtctgcaagt
     1441 caagtggtcc taagttaatc cacgacctag ctccaccttt tactgtccca attccaggta
     1501 ataaaactat ggctaacaaa ataagactta ttatataaag cgctttataa tacctgccta
     1561 gaaggttata gtcaaaaaat aatatgacta taatcattcc tatcccaagt acaaaagcta
     1621 atccttgttt aatcaattga ttatatgaac ctgttgaatt agcatgtgtt gcactactaa
     1681 gtataacaag cccaaaaata aagatagcta atactgtaac tataagtttc cagtctaact
     1741 gttttatcaa cttaattgtc gacttataat taatattgat ttttttcaaa gaatcaccgt
     1801 ctttctatct ttaaacatat aattaaataa aaggggcata taccaataag gatatccccc
     1861 ttttaagcac taattacata catctatctt ttatattctt tagtggtata ttagcaacta
     1921 atgcagatac aggtttgtca tcatgctcac ctctgcattt agacattttt atttcaagac
     1981 catcatcttc tatttctgca taattagcta ttactttcaa aatgtctgtt tttatcatct
     2041 ctaaaagttg tggtgaacaa tctactctat catgaactaa aactagcttt aacctctctt
     2101 tagcaacaga tttactagtt ttagcctcgt tagaaaaaac tctaaataaa tctaacacag
     2161 atgtatcctc ctttgctact tagccatgct aaacattttt ttaagtctac caaagaatcc
     2221 atcttcaact tcaagatcaa gtagaggaac ttcttcattt agtatccttt ttgcgatatt
     2281 tttgtatgct tgaccagcaa gtgacttaga atcaagtata gctggctctc ctttatttgt
     2341 tgatataatt atgctttcat catcaggaac aagacctaac aaatctattg ctaatatttc
     2401 tattatatct tgtttatcca tcatgtctcc acgcttaacc atatcatttc taattctatt
     2461 tataactaat cttatctctt ttatttcatt tgcttctaat agacctatta ttctatcagc
     2521 atctcttact gctgatattt ctggattagt aactacaata gctctatctg ctccagctac
     2581 tgcattttta aagccttgct caatacctgc tggacagtct attattatat actcgaaaga
     2641 ttcttttagt ttctcacaca ggtcaatcat ctgctcaact gagacagcat ttttatctct
     2701 agtttgtgct gctggtaata aatatagatt atcaaatctt ttgtctttta ttagagcttg
     2761 cttaagccta caagttcctt caactacatc tactatgtca taaactattc gattttcaag
     2821 acccattact acatctaagt ttctaagtcc aatatctgca tccacaacta ctgttttttt
     2881 attttctagg cttagcgcag ttcctaagtt tgctgcggta gtagtttttc caaccccacc
     2941 tttaccagat gttataacta taacttcgct cataatacta ccccctattt ttctttctat
     3001 ttgtctaact ttgacaaata gctttcgatt acaattcttc cattacttac aaaggctatt
     3061 tctgggctaa tttctgattc actttcataa ttttcttcat catctggagc ttctgcaata
     3121 ttatctgcta tttgtaatat tttaggatta agatttccag caacaacata tgcaaattca
     3181 ttgccttctg cacctgcatg tacaaaacct tctattttac ccattacaaa aacattacct
     3241 cctgctacaa ccttagcacc agcatttata tctcccataa caactgtatt tgattttgaa
     3301 aggacttttg aaccaggttt catatcattc aatataacta catcaccttc aaattcaata
     3361 ttttcaccag atcttagggt attgacatat tttgttggaa aattactatt ttcttcaatt
     3421 ttttgatctt caacaaattc cacatcaaat ctagaagtaa ttccttcttt aagttctaga
     3481 atctccatgt cagttaaaca atcactatta attttactaa ttttagcccc attaaaaaag
     3541 ccaacatatg cttccaattt gtttattatt ttttcctgta tttcctcaaa tggagcttct
     3601 ctttttatat ttactattat ccctctttta ttacccttga actctactag ttcttgacta
     3661 caaatttctc ttaaagacat aacaacctcc agagtgatat atcatttata ttcttcaaat
     3721 acgctttaaa cccttttatt ttttctatat aaagctaaag tttatacata gattgagcta
     3781 tctattaatt tgtgactcga aatttatatt ttcgtcagaa gaattgttaa catcattcgt
     3841 cttgttgtca tttttactat tagttggctc taatcccata taagttccaa taacttctct
     3901 agttggtaaa agtgagaata cacttgaatc accttgagga attacacata caacagctat
     3961 ttctgggtca tctgctggag caaaagcaac agtccacgta aaagaaccat aatcttgttt
     4021 aaatctatct atttggtcat ctgttatttt tggatttaat tcttttatag cttttcttag
     4081 atatgaagaa ttcaccttat cagtgtcctc taatctaact tttactccat cttctaattc
     4141 ttcctctaat ttctttcttt cttcgtcttt caaatcttta ttttctaatt tcttttttat
     4201 ttccttttcc ttagctaatg aaagctcttt ttctttttct gctttcatct tatcagctag
     4261 tttaatcgca tcatttaggt tgacattata agatgacata tgacttttta aatattcata
     4321 ctcgttatct gtaggtattt tcccactttt ctcagctgta ccagtttttg ctgctacatc
     4381 tattggaaaa tcagcaaaag cactcttcgc tgtaccttgt cttacaacca atttcatacc
     4441 ttttgttaat tcttttaagt tatctggatt tttaaaaggt attttttcga ctttcttctg
     4501 gtcattaatt ttaactgatg aataatcact tgaaacagct ctatcaacta cagataattc
     4561 tactaaatta cctccatttg ctatagcagc tacatatctt gatatttgag ctggtgtata
     4621 agcattttca ccttgaccta tagctaagtt aaatgtatct gcagtactcc attttgcaaa
     4681 gttaaaataa ctaaatactg ctaaatctgc tacttcttca attctatctt ctttaacatc
     4741 caattttttt agcctattca ttgtttcaac tctaccagga gttttctttt ctgctgccca
     4801 gcttacaatc tcattgattc ttttctcata ttctttagga tttttttctc tagttatatc
     4861 aacaaaatca tttgccattt ctctttctaa atttgacttt agaagagctt gtgttgattt
     4921 caatttatct tctgtgctag gaactttacc tgctctttct tctacttcat cttgaagccc
     4981 tgtattttgg tcaagcccaa ataattttgc atactcaagt attttatttg cattcatatc
     5041 tataccaatg ctttttccat ctggccatgt tttacctgta ccaattgttg ccatatatat
     5101 attacaagac tcttgtattg ctttatataa gtttgtcata ccatggtttc ctctaccatg
     5161 gttccataca tagtcaccaa aagatttttt acctaacatt ataaccccag ggtcatttat
     5221 agtaaagttt ggatccagac cattttctaa cgctgccatt gaagttagca tcttaaatga
     5281 agaacctggc tgaaattccc cttgtgtaac caagtttaaa agtggacttc ctgctagtaa
     5341 gtcatttgga ttttttggtt gaagtgcttt ataatcctct gtagaaatac cttttgcaaa
     5401 tttatttggg tcataattag gataacttga tgaagctaat acttctccat ttttaacatc
     5461 aatagctata agagctgctg actgtgcttt tcctgcatat gcactaattg gtttgtcccc
     5521 aaatttactt ttaaaagtac ctcctttaga agcaacttcg attatctgtt ttaatgcatt
     5581 gtctgaaact tcttgtaaat ctttatctag tgtaagatac acagtatctc ctgatttagg
     5641 ttttttagat tcaatttctt tactgattct acccaatgca tcaactttaa ccattttata
     5701 accatcagtt cctcttaatc tactttcatt actcttttct actcctgcaa gacctaccat
     5761 gtctccagtt tcataacctt tttgaaggta tgattctatt tgagtggatg gcatttttcc
     5821 tacatatcct aacatatgag aagctaatga cccattagga taatatctta ctggttctac
     5881 tgctattgaa acaccaacaa ggtccatagc actttcttca atttgagcaa tcgtagtttc
     5941 tcctacatcc ttagcaatag ttactggatt atattgagaa tatccttttg attttatcaa
     6001 atcccttacg accattattt ttcttgcatc ttcatcactc aaacttttat caatttctaa
     6061 tgcatcacta tttcttactt tttcaaacgc ttctttggca ctcagtttag tttcttttat
     6121 cttataacta gccaaccaat ctctcttatc tctttctgct gtgaacatcc atttactaac
     6181 taaaataggt ggataatatc cattttcatt tagttttgcc tgtaatcttg tcgcatccaa
     6241 tcttttatct tcttgagata atattccagc tgatataagt ttatccacca agtaataaaa
     6301 actttcctta gcattataat catttggaat accattttct gatttatatt ccctaatatc
     6361 tctgtcatat gtataatagt actttccatt ttctacatat ataggaaact catctacata
     6421 ttcttcacca tttctctcca gcaaatttat aagttttaat gatatttcat ttgctcttga
     6481 atgcttattt gcatctttct tattaatatc gttacctgaa acttgaacag taaataagtt
     6541 tttatttcct gccaacagtc ttccatatct atcttttatt tctcctcttg gagcttctat
     6601 tgccaacttt ttatatgttt tattctcagc taactcattg taatattcat atttaaatgt
     6661 agtcatatag attattttta ctaaaatagc taaaaatatc acgcccatga ctatcttaat
     6721 tatttttagt ctatcaactt gtttattttc ttccatgaaa tcaccaacta atcttcttta
     6781 agttttaaaa ctgctctttt agataagcga tatattacta aaccaattaa actattcact
     6841 attggtgcta ctagtagacc ttttacaaaa agtgttgata tgttgtagct ttgataaatt
     6901 atactagatg tacctatatt gacaattgaa tcaattaaac tgcctataaa aattaaaacg
     6961 aaagtaataa tataactacc tttatatagc tgatctctca tgtatccaat tgcatatgca
     7021 ataataaaca aaattaatgc atttacacca aaaattccac caacggttat gtctttaata
     7081 agccctatta ttgctgctat aatcccacct tctaattcat ctaaataaag agagattatg
     7141 gttacatata taagaactaa attaaagctt atgccaaaaa tatctatata atttgttata
     7201 ctattttcta tagtcataag cacaatcccc aacagacaaa gtaaaacttt tttcattgtt
     7261 tttatcccct ttatcctata tttcttggtt ctatgactac aacgtcattt atattcttaa
     7321 aatcaacata aggttttaca actacatatt ttaatgactt atctttatca tcaaccactt
     7381 tttcaacttc gccaattggt ataccatctg ggaagaatcc aagacctgat gttgttatta
     7441 tatctccttg aattacatta tatgatgagt caaacatata tccttgcaaa tatcctttac
     7501 tattataact atctttatta tctaaagtag tattttgagt aattgtacct ttagcattgg
     7561 catcctttgc tagtttaaag cttacagatg ccttagaatc caatagtgaa attgccttac
     7621 tataattgtt ggaaacctca taaactatgc caactaatcc actaccattc ataactatac
     7681 tttctttttt tacacctgaa ttttttcctg cacctatgac aaaactctca taccagtttc
     7741 catcattttt accaacaaca cttgctgaaa tacttgtcgc cttatactct tcttgtacaa
     7801 agttcagtgt cttttttaac tcttctagcg attctgtttt atctagtttg gcatttaaag
     7861 ctatcatatt ttttttaagt ttttcatttt cttcttctaa tttttttacc tttttagaat
     7921 tatctttaaa atttgctaca tctttaaaac catttttgat aaaagaaaat ccatcattta
     7981 ttcccttacc tacacttgta atagcatctg aagctactcc taaatttact ggactaccac
     8041 tagaaaattt accaattgat atacccacaa ttccaattaa agtgatagca actacgcccg
     8101 ttgctatcac tttaacatta atcctttttt tctcattttt gttcttgtca aatctcaagg
     8161 ccatcaccaa cttctaattc ttagtattca tcattaatac tttttcaaag atttcttggt
     8221 cttcaacaga tttacctgtt ccaagtgcaa cacaatcaag aggtgtttct gctatttgta
     8281 cagacatacc agtttcctgt gtaataagct tatcaagacc tctaagcaaa gcgcctcctc
     8341 ctgttagcat tattccgttt tccattatat ctgaggctaa ttctggtgga gttctttcta
     8401 gtgtagattt tattgcatct actattgaac ttacaggttc ttttaatgct tctctaactt
     8461 ctgtagagca tatttctatt gttttaggta atcctgagat taaatctcta cctcttattt
     8521 gcatatttat ttcttcatca tctttaaatg tagaacctat gttaattttt acattttcag
     8581 cagttctttc acctatcata agattatatt ctttttttac ataagccact atagactcgt
     8641 caaattcgtc tccacctatt cttatagatt tagcagtaac gattcctcct aaagatatta
     8701 cagctatttc tgaagtacct ccaccaatat caactaccat attaccttct ggttcttcaa
     8761 cttttaatcc agcccctata gccgctgcca ttggctcttc tataaggaat gcatctttag
     8821 ctcctgcttg tcttgcagcc tcttctatag ctctcttttc tacttctgta actccaaaag
     8881 gaacacatat agctattctt ggacttacaa cacctttttt atcagccgct ttttgtataa
     8941 aatagctaat cattgattga gttatgtcaa aatcagctat tactccatct ttcataggtc
     9001 ttatagctac tatatttcct ggtgttctac ctatcatttt tttggcttct tcacctactg
     9061 ctaatacctc tttactatca tctcttattg cgacaactga tggttctcta acaacaatac
     9121 cttgtccttt tatgtaaact aatgtatttg cagtccctaa atctattccc atatcctttg
     9181 tcatcttatt aaaactaaaa aaaccttttt tttctttctt tttttcctta gccatgattt
     9241 tactccttcc tactattaag aatcatgttt aaaacattct cctattattt tatcacatat
     9301 aaagtcaaat tatcatattt tctttaaagc taaagtataa tccatctcca ataattatat
     9361 ggtctattat ttctattcca attatttctc cacatttaat aagtcttgat gtgatatttt
     9421 tatcttctct tgatggttct acacttcctg aagggtgatt atgcatcact attatcttac
     9481 ttgcacttct tcttatagct tctttaaaaa cctctcttgg atggactaat gacgagctta
     9541 aagtccctat agatacatct acatcagata ttatctcatt ttttgtgttt aaaagaaccg
     9601 ctttaaaaac ctcttttttt aaatacctta ggctatccat ataatacctc tgtatatccc
     9661 aaggattcct tattttatac tttataggtt taaaactagc tactctagag cctagttcta
     9721 aagctgcttt aatttgggtt gattttgata acccaattcc atctatatta cacagctcct
     9781 ctattgtcat atcttctaaa tgtctaatac cctgaatatc tctatttatt atatagttgg
     9841 ctaattcaat agcattttta ttcttgttgc cagttcttaa aagtatagcc aacaactcag
     9901 catttgataa gctttttacg cctttggcaa gcatcttttc tctgggtctt tcctctggtg
     9961 ccatttcttt taccttaatg gttgaattaa atgacttttt cagataaatc acactccata
    10021 aaaaagattt atactaaaat atttttttaa taggtcgcct aaccttgaaa ttggaagacc
    10081 aactatatta aaataatctc ctctaatttc ttcaactaac aaagctccat aaccctgtat
    10141 accataggct cctgctttgt caaatgactc attagttttc agataatcct caatatcctg
    10201 ctctgataac gttttaaatt tcacattgct gattacatag tcaattacct ttttatcttc
    10261 gcacaagttt attaagctaa tacctgttat gacttgatgc tctcttccag atagtctttt
    10321 taacatatct cttgcacaag actcatcttt tggttttcct aaaatagcat tgtctaaaac
    10381 aacaactgta tcagctccaa taacaacatc ttcattatgt tcagatgcta cagacatact
    10441 tttttcaaaa gctaatctca taactaaatg ttttggtgtt tcaccttcca atataatctc
    10501 atcaatttca tttttaatta tatcaaatct tacattcgta ttctctagaa tttctttcct
    10561 tcttggagag gctgatgcca agattatatt catttattca cctcatttgt acgtaatttg
    10621 tacctattta gtctatcact atattgtaaa aaattcaaca gaaatgtcga accttttttg
    10681 ttacactatt gttatattta ttaccattag aataaatata cataaaatag aggtatggaa
    10741 attattccat atccttattt tgtccatatt acaaaatttt aatagctttc tttggacatt
    10801 tttccataca taaatggcat cctacacatt tatctgcatc taccttatgt ttttctttta
    10861 attctccctc tattgcatca aacttacatt gttttttgca cactgtacat ccaacacaaa
    10921 gttcttggtc aatagtaact ttctttttct tagttatgtc acctgatatt acttttgtag
    10981 ggcattttgc tacacattgc atacaaccta cacacttatt agggtctatc ttagctattt
    11041 tgtcttcaaa tattattgca tcaaatttac aagcttttac acacattcca cagccaatac
    11101 atccagcagt acatttttct ttaacagctt taccaaactc tttgctatta cactctacaa
    11161 ctacttcttg actttctggt tttgtgatta ttataccctt aggacaaact tctttacatt
    11221 ttccacaatt tacacacttt tcttcatcta ttactgctat tccatcaact atagaaatag
    11281 catcaaattt acaagcatct ttacatgttc caagacctaa acatccaaat ttacacatct
    11341 tagcacctga gtttaaaaca tttgctgctc tacaatctga tattccttca tactcatatt
    11401 tatcttttgc acttgaacaa gttcctttac aaataacttt tgctacttgt ttctcacctg
    11461 cttcagcact tattcccatt atttctccaa cttttgcacc aacatctgct ccacctacag
    11521 ggcaaccatt aactggtgca ttgccttcaa ctatcgcatt tgcaagacct ccacaacctg
    11581 gaaatccaca acctccacag ttggctcctg gaagtactcc tagtatagct tccactctct
    11641 cgtctacttc tacagcaaac tttttagatg caaaatctag tactatcccg aagattaagc
    11701 ccataattcc taaaactaat acagctgtaa gtatcaccat tttcacccct cttttttata
    11761 gctttataag tcctgtaaat cctaagaaag ctattgacat taaacctgct gatataagcg
    11821 ttatagggaa acctttaaat gcttctggta tatcagctaa ttctagtctt tctcttatac
    11881 ctgcgaatat tactattgct aaagtaaatc cagctccggc tccaaatccg tttattattg
    11941 tttctactaa attataactt ttttgaacat taactagagc tataccaaga actgcacagt
    12001 ttgtagttat aagaggtaaa taaactccta atgcttgata taaagatgga ctcatctttt
    12061 gaattaccat ttcaacgaac tgaactatag aagctataac caatataaaa gctattgttt
    12121 gtaagaatcc agttccagtt tttactaata aaatctgtat aaaataagtt ataattgaag
    12181 ctagagttaa aacgaatgta actgcaactc ccatccctac tgctgtatca accttcttag
    12241 aaactcccaa gaaaggacat atacccaaga attgagatgt tataacgtta ttaacaagaa
    12301 cgatacttaa aaataaaagt attaaattca tttattacac ctccactatg cttttttact
    12361 tcttaattta ttaaatcctg caaataagaa tcctaatgtt aagaatgcac ctggtggtaa
    12421 tataaatatt aatactggtt ggaaagatgc tccaaataat gtcattccaa ataaactacc
    12481 atttccaagt aattctctta ctgcacctat tattgtaagt gcaacagtaa atccaagacc
    12541 ttgacctaca ccatcaacta ttgaaggcat agctcctgtt ttgaaagcaa aactttctgc
    12601 acgagctaat attaaacagt taacaactat taatggtata tatataccaa gagccttatc
    12661 aagagcagga acatatgctt ttaaaagcat acctactatt gtaacaaatg tagctataac
    12721 tactataaat ataggtattc ttatcttatc tggtgtaatt tttcttatta aagatataac
    12781 taaatttgca catattaaaa ctgctgctgt tgaaagaccc ataccaattc catttattgc
    12841 tgaagttgta acggctagtg ttggacacat acctataact tgaacaaatg ttgggttctc
    12901 atctattagc ccatttttaa atactttagc taaattcata attaaccctc cttatttatt
    12961 aagagcacta ttgaaaacat ctatagcagc atttactcct gtagtaactg ctgtagatgt
    13021 aatagttgca cctgatattg ctaatatttg attatctgct gttgtacttc cttttgctac
    13081 ttcaagaggc tcagcagttt ttcctttgta ttgaccctta aatgcatcat ccttagcttt
    13141 tgctccaaga cctgctgttt ctgacatatt accaatgtca acacctgtaa cttgaccttc
    13201 agatgatata cctaccataa cttcaatttc tccaccatat ccacttggtt tagcttttaa
    13261 agtatatcca accacttcag aaccatctaa accttcatac acttcagcta taagaccatc
    13321 tccaaggcct ttgtaagtat cttccttcat ttctttaaat tcttttgctt ctggaaggac
    13381 tgcttttcta agttcattat ttgcttgtat atttctttgt tctattactg gagctgtaac
    13441 ctgattagta gctccaagag ctaatgcagc tattgcactt atagcaagta aagtaccacc
    13501 taatctcacc atactgttca ttatttagcc acctccccaa atactctatt tctaacaaac
    13561 ttatctataa gtggagcaag acagttcact aatagtattg agtaagatac accttctgga
    13621 tatccaccat acattcttat aacagttgtt ataagaccag ctaatacagc gtatattatt
    13681 tgaccttttt tagtcatagg cgaagttgta taatccgtaa gcatgaagaa tccacctaac
    13741 ataagtcctc cagcaaacag ttgatatatt gcgaaagtta tattaaatcc acctaaaagg
    13801 aatgttaata taaatacagt tgctatgtaa aatactggta taacataatt tattatgcct
    13861 ttatacatta ggtatactcc acctaataat aataatattg ctgatgtctc acctatacat
    13921 ccaccaatat tccctatcat catgtcagca agactaattc cattgctaga aaggtctgcc
    13981 agtcctgctg ctccattttt caaaaagctt aatggagttg ctgtagtaac tgcatctaaa
    14041 tttttaccta tccagttaac acctggttga gtccaagcag tcattgctac tgggaatgat
    14101 gctaataaaa atgctcttgc agcaagtgct ggattgacaa agttacatcc aattccacca
    14161 aataccattt taactactaa tattgcaaat attcctccta ctagacacat ccaccaagga
    14221 agtgatgctg gtacgttaaa tgctaataat aatcctgtta caacagctga aaaatctcct
    14281 atagtacttt tttgcttcgt aaatttttga tataaaaatt cagtacctac tgtaccaagt
    14341 atacaaaaaa acatagcact caaagcatta agtctaaaga agtatactcc tgctactgct
    14401 gctggaagga gtgctataat aacttgtttc attatatatg aagtatcttc attacttctc
    14461 acatgaggag aagatgatac tatcaactta ttttccatcg tttttccccc ttaactttct
    14521 ttatttctga gcagcttgct ttcttctctt tgctcctatt tctctctttg ctgatcttat
    14581 agattgaagt aatggtcttc ttgctggaca aataaatgag caagacccac attctataca
    14641 atccattgct ctatgatatt cagctgtgtc atagtcatct tttaaagaat atgcacttat
    14701 gaaaagtggt tgtaagaatg atggacatac atctgtacat cttccacatc ttaaacagtt
    14761 ttgaatatct ggagtacgtg attcttcctc atccaaacat aaaattcctg aagaaccttt
    14821 gttagtcgct atttcagtag tatattgagc tattcccatc ataggtccac ccattataac
    14881 cttaccaatc ttcttatctt ctttaaatcc accacattgt tctattattt ctgaaactaa
    14941 tgttccaact tttgttatta ggttctttgg ctctttgatg caactacctg ttattgtagt
    15001 gattctttca accaaaggca tacctgtttt aatagactta gctatctgtg ctgctgtggc
    15061 aacgttgtca acaactgcac ctgctgctat tggtaatcca ccagatggaa cttctttgcc
    15121 tgtacacgca taaataagtt gtttctctgc tccttgtgga tatttcactt gaaggccaac
    15181 aacttcgatt tcgctatagt ctttagctac attttgaatt gcttctatag catctggttt
    15241 attcgtttca attcctataa atcctttttt gacatctaat accttcatta atgctcttaa
    15301 accaaacaca acatcttctg gattttcaac cattaatctg tggtctgcag ttaagtatgg
    15361 ttcacattct gcaccattta atataactac ttctgccttg ctatctggtg gaggagatac
    15421 ttttacatgg ttagggaatg tagctccacc catacctact attcctgcct cttttatgat
    15481 acctactata tcttctttgc tcaaatcctc taactgacct tttggttgaa cactctcatg
    15541 taattcttcc ttaaagtcat tttcgataac tacacattga gctgaacctc ctggtacttc
    15601 atgttgttca atagcaataa ctttacctga tactgaagaa tgaacgttac atgaaacaaa
    15661 accttgttgt tcacctattt tttgcccaaa ctttacttca tcaccaactt caacaatagg
    15721 tttagctggt gcccctatat gttgctgaag aggaatgtaa actatctttg gagcttgagc
    15781 tttttcaagt gcctttgtgt tagagtactc ttttctatat ggaggatgta tccctccctt
    15841 aaaagttaag agtttcattg ttttaccccc ttatctataa aaaaacattt tttctgcctt
    15901 ctataagtat actatatata ttaaaaaatc ttaacaattg ttaaaaaaat atccgtattt
    15961 ttataattta ttttataaat caatacctct atattaagtc aaaccgagga aaaggattgc
    16021 ttaattaaac aatccttttc atatagcttt ctaaagtcca aattttctgt attttatttt
    16081 atagaattta taaacataga gtactcctgc atggatgata aaagcagact tttacttaca
    16141 ctatattttt cttcatttgc tgcttttgaa gctgtatcaa ttttttcatt cacattcttt
    16201 acatactcta ttaaattatc tttaaataaa cgcatttcag aatagtcaat cttttcagct
    16261 tcttttgata tattttgtga tattgccttt ctatttgtca aaatagtatt atatttttcc
    16321 atatctacat tattttcagc attcgaccaa aaacttgatt cttctttaaa gtttgttatt
    16381 aatttattta attctttaga tatactctct atataagcgt agttacttgt atactctaaa
    16441 gacaacatag gtgcatctaa gtgtgataaa aatgcatctg gaaacacctt tcttacactg
    16501 cttatctgct ttctaacatc attttcatca aaagaggaat aggtctgtat tttttttact
    16561 ccatccttct ccataactga aaaaggtatg tcattattaa ctaatgatgt ctgtattttt
    16621 ttcaaatcat cattattatc tattgcagca acttgaatag tatacatatc ccagccttct
    16681 atagtagcta atttgacttc ttctggctct ttagtgttat ttaccgcttt ttctggatta
    16741 gtttcttgtt tatcatctga attggtttgt gatttagatt ttttcccttt ttttattgaa
    16801 tttaattcat cagatatatc tttataagtt atatctgatg aattatttag aaatggtatt
    16861 ttagccgata catactccaa tatcttgcta tcttttattt ttgtataaga ataatagcca
    16921 attagacaca agcaagcaat cgttataaca tatttacgta aatcaatctt atgtctatta
    16981 ttattaaatc ctctgtatat cgtttttctt ctattcatta gtttgtccct cccaaaatta
    17041 tattattaat aaagtatatt tttaagagtc acaatttatg aatataaata attatttctt
    17101 ctaatttttt aataagaaat tgtacttact aaacaaatct tcttgcacca agtaatactt
    17161 tagaccaata tgaactagtt aaattatcta ctttaacctt attaggatta gtctgagtac
    17221 cactacaatg gataaattta ctatttccta catatatacc tacatgatta gttgttccat
    17281 caccatcagt gtcaaaataa actaaatctc ctggtgcata attccccata gaaattgcac
    17341 ttccaaaatt agcctgttct ctagaaactc taggaataga aacgcctaca gaatgtttaa
    17401 aaacatactg tgtaaatcca gaacagtcaa atttatcagg ccctgaagct ccccattgat
    17461 atggtattcc aataagagta tatgcaaaat ctacgattgt tgaacctttt gctgtagatt
    17521 tatctgaatt tccagtaaat gatggctttg agttactatt agattgagaa ttttggttag
    17581 atgatgaatt tggtttttct tttgtatcct cattagtctc agaaatataa ctggcactca
    17641 cccatccaat tgttccactc gataacttta ccttatacca accattactt ttagctttta
    17701 attcaacaac atctccacca tttaattttc ctattataga atagcttgtc ccaggaccac
    17761 ttcttacatt taaactcaca gcagatgtta ccttaccatt ctttccttca attgattctt
    17821 cgctagatgg ttttttatca gaattattag tactatttga attactagaa ttgttcgttc
    17881 caacatcctc tgatgtttta gagatatatt ggctactagc ccatccagta actccatttg
    17941 atagttttat tttataccat ccattgcttt gttctagtaa ctttactaca tctccattat
    18001 ttgctttacc aactaatgaa tagttagtac ctgcaccact tcttacattc agtctagaac
    18061 tcacatttac tttaccattt ccactaatag ttgtcccact tgaagttgaa ttattttgac
    18121 ttgatgtacc ttcagacgaa ccacttgttg atatgtattt tgcacttccc catccaacaa
    18181 cagaactcga tacccttact ttataccatc cattagactt ttctaaaatc tctacagttt
    18241 ttcccttgta taattttgct accttatcgc aatcagaact tggtccactt cttatattca
    18301 aggcacttgc tgttactgtt cccttttcaa gagcacttgc atttatggag ctaacagata
    18361 ctgctactgc cccaattcct aaagctgcta tagctttttt cactactatc actcccccca
    18421 acaaattttt atagtcataa attcataaca ttattatcca tttttagtcc ctacaattta
    18481 atgataactt attttcttat taattgtaaa gaaattataa acatatcctt aaaattagtg
    18541 atttttagga agtatttcaa tccaataatt atctgggtcc gctataaaat aaattcccat
    18601 actttcattt tcaaaacata tgcaatctaa atctttatga agtttttttg cctcatcaaa
    18661 ctcctcaact tctaaagcca gatgaaattc attgtctcct aaattatatg gtctatccca
    18721 atctctaagc caagttaatt ctaatgtatg actagtaatg ccatctccta aataaactaa
    18781 tataaaactt ccatcttctg cttccttgcg ctttacctct ttaagcccta aagctttttt
    18841 ataaaaatct aaactttttt ctaaattagt aacattgaaa ttgttatgac aaaaattaaa
    18901 cttcattttg aaatcactct ccaattttta ttttttttat attatatcat tattataaat
    18961 ttatttaaat catattaagt taaaaatttg aatgatgatg accaattacc aaatcatggt
    19021 aaattattta aaagaatatg tcaaaaaaag aataagctct ttcaaatgca taaaaaattt
    19081 atacccaagt tatacttaat catgcaaatt ggcagaactt attctttaaa actctaactt
    19141 ttaagttttt ctttaataat ataatttatt actttttttc taatatcttc agcattataa
    19201 tcatcttcta cttctatatc caaaagtttt gctattgacc tcatatcttc tctagtcttt
    19261 gacctatagc ttgatattaa ttcatctttt attttctctg gttctaaaag atattcttca
    19321 tctcctcttt tgaaaacaac gtatttttga gcatatgaac ttctattgta gtatatatat
    19381 cttgatattt ttcttaaaat ttgattatat gaaagtcgct tgttaatatc aagttttaag
    19441 tactctgaaa aagcaaatag ctcttttttt gatttaaata atgcttcttt ctttatagtc
    19501 cttgatattg aagttacact ttcttgatat gcacactttt ttaacatttt taaatatact
    19561 ctattaagac caatactttg attgaatttt tttatattat tattaataat cttatccaag
    19621 ttaccttcaa agtatttttt tgctcctttt tgtgatacat aactctcaag ttctttgtgg
    19681 gccatatcaa gtatcatttt ttttaaatcc tctttatatc ccctagattc catgatactc
    19741 cctcccttag ctatttatag tatattcaaa aagctcaaga aatgtgagtc attacaaaat
    19801 ttttttactt tttatgtata tattaattta aaaggggaga taatagtaat gtaaaatcaa
    19861 acatactcaa tctcccccat ttttagacct ctattactcc cccaatagag gtcttttttt
    19921 agtttatcaa tttctattca attcccttta gttcaaatcc taattctgct atcacttctt
    19981 ttaatttttc aatagataca tcatctttca tatctacaga tgcacactta ttttctaaat
    20041 ctacatctaa aacttcaata ccatctatat cttctgttaa tgcagtttta agatggttta
    20101 cacaatgacc acaactcata ccttctacta atagtttctt cttcatttaa ttacctcctt
    20161 ataccctata agggtattct atcatatagt ttatactttt taaatagaaa ttaatcattt
    20221 aatttgattc attcgatttt atatctaaat tagatattcc ctcatataca tcaaaatcat
    20281 ttggagtaat tttacctttt aatctactat tacttacaat atagttgttt ctcacagcta
    20341 aacttataaa tgttgcatta tcttttatat atgtctgtac atctttgtat gctttttttg
    20401 tttcaatttc agacgtagaa cttgtaagtg cagacatata tccatttaac ttatcatcag
    20461 tatatccact actcgctata atactacttg catctggtat aattgatagt ttccatccaa
    20521 ctaatgctaa atcataattt ttagaattaa gagcattttc catatcctta tctgacagct
    20581 gatttactgt actatttatt cctatagctt ttaagttttc atttattatg tttgcagttt
    20641 ttattctctc actattatct ctgtttacta ttatctttaa gttcatttca gaaagacttt
    20701 tctttacttc ctctttttct ctattttttt tctcttcagc ttcttttttg attctatctt
    20761 cttctgcttt tttagctttt gcttcttctt ctggcgtaag cttctttgag ttctttttag
    20821 ctgatttttt ctgatttttt actttattgt cattttgatt tactgagttt acatttgcat
    20881 attctacctt agcaagacac tctatcgcct tatctttatt aaactctaaa tccttcattt
    20941 cactattata gtattttgat tttgaattaa gtggaaaatt aactggagta gcatcatcca
    21001 tataaccttc actaataatt ctatctttat ctatagagtg tgcaattgct tttctaaaat
    21061 taacatcttt gaaaaaagga ttattataat taaataatac acactcataa tctcttcctt
    21121 gatattttgt tatattaaac tctttttctt gaaacttact taaatcattt aatgtaacat
    21181 tggttatatc actatcaaga gccataacca tagatgtcct tgcatcttca ttaggcacca
    21241 tacctacttt tatgttcttc atggtttttg gaacttcttc atagtaatct ttgtttacag
    21301 ataaaaccat tccttctctt tctgtatatg attctatttt atatttccca tttcctatca
    21361 tattattatt cttgtcattt acatcttttg aatctaaatg attttgagat acaattggaa
    21421 aaattaaact atcaattgaa aaagcatatc tagctttaaa ttttattgta aagtctttat
    21481 ctgatttaat attcacagaa gctatatttt tagtaaattc attatatgga ctatctgcac
    21541 ttttttgaat taaaccaata gtatagttta catcatttga agtaacattt tttccatcat
    21601 gccactttgc atcttttagc tttatatcta tactcattcc atcctgagct atattatact
    21661 ccttaactaa ttgaggaatt acattgtagt tctcatctat agtaaataaa ctatcataaa
    21721 ctaaattcat tatataacca acagacttat cagtattagt tataggattt atagtttttg
    21781 gagttaccat tgttaagttt atatacttag catctataga actttgactt tgtaaacttg
    21841 aatcactact actttgtttt gttttagtat tactacaacc agctaccata agagttatag
    21901 ctaaaacgac tgttaagact tttattctct tcattagttt tttcctttct tgccatatat
    21961 cttattataa tgttcgtaat tattttttac tttttctaca tattttgaag tttgcttaaa
    22021 aggtatatca tgtagatttt cgccatcttt actatattca ttattttcta accatttttt
    22081 cacattacct gaaccaccat tatatgcagc tatcactaaa tctaatttac caaattcttt
    22141 gtataactta cttaaatacc aacagccaag tcttatatta gtctctgggt cgaaaatatc
    22201 cacatttttt aaatttagtt cctctgctcc ccaatctcta gttatgtcta atatttgcat
    22261 taatcctttt gcttcctttt ttgaaacagc agaactatta aacttacttt cggctttaat
    22321 aacactgtaa actatatttt catctaaatt aaattctttc gaatactttt ctacatactc
    22381 tgaatatttt ttaggatata aaaatttatg tattactttg ctttccatta atagtgcccc
    22441 aaataagatt ataaaaatag atagaatcaa tactttctta ctattcacgc caattctcct
    22501 tcatgtactc aataaattta tgtgctttac tttctaattc agttattgtt cccgaattat
    22561 ctattatata atcaccatat ttggattttt cttcctgact catctgtgaa tttatcctac
    22621 taagtgcttc ctgctcactg caattatctc tcttttgtat tctactaatt tgtacttctt
    22681 gtttacaagt aactactaat agcttgtcaa ccatatctaa aaaaccactt tctaccaata
    22741 ttgccgcatc taacactact atattttcgc ctttttttct caatttttct atttcactta
    22801 ttattttttc tcttattcgt ggatgagtca agttatttaa ttccttaagt ttttcttcat
    22861 cactaaaaac tattttccca agagcttttc tatttagagt tccatcatca ttttttatac
    22921 tttgcccaaa atggacaaat attttttcca ataacaactt atcctcaaat atcttcctgg
    22981 atataatatc agcatccact ataggaatgt taagatttct aaatatattt gataggctgc
    23041 tttttccaca gcctattcct cctgtaagtc ctaatatcaa cataaaatca cctactttgt
    23101 ctcataccaa gaactaccta catttaaatc tacatctaag tgaacatcca tatttacagc
    23161 attttccatt tcttctttta ctattttctt aactatatct atttcatcat caattgcttc
    23221 aactatcaac tcgtcgtgaa cttgtagtat aagttttgat ttcaaattgt tctcttccaa
    23281 ttttttatga acatttatca tagcaatttt tattatatct gctgcacttc cttgtattgg
    23341 tgcattcatt gcaaatctct ttcctctatt tctttccata aaattgcttg actttatttc
    23401 tggtatatat cttcttctgt ttaaaattgt tacagcatat ccatcctcac tagccttttc
    23461 tactgttgtg tccataaact cttttatttt atggtattta gcaaaataac tttcgatata
    23521 ttctttcgcc tttggaacag gtatatttaa atcatcagct agaccaaagt cacttttacc
    23581 atatataata ccaaagttta cagctttagc tgcacctctc aattcactag taacatcttc
    23641 cataggaaca ttaaaaactt gtgaagctgt tttggtatga atatcttcat gatgtttaaa
    23701 tgcttctatc atattttcat cctgactcat atgtgcaaga actcttagct ctacttgtga
    23761 gtaatcggca tcaaccaact tacagttatc atctgcaata aacacttttc ttatttttct
    23821 acccatttcc atttttatag gtatattttg aagatttggt tctgtagatg aaattcttcc
    23881 tgtagtcgtt atagtttgat taaatgatga atgtattcta ttactttctg gatttattat
    23941 atttaaaagt ccttcaacgt aagtagaatt taactttact atttgtctat actcagttat
    24001 cttatctatt attggatgct tatctcttaa tttttctaaa acatctgcat tagttgaata
    24061 tccagtttta gtttttttaa ttattggaag ctctaatttt tcaaataata taacacccaa
    24121 ttgttttggt gaatttatat tgaatggctc tcctgataat tcataaattt ccttttctaa
    24181 tgtagctatt atttctttaa attcaacact caattcatcc aatatacttt tgtctacttt
    24241 tataccttca tactccatat gacctaagac ttctactaaa ggcatctcta catgataaaa
    24301 aagaccatcc atactcatat ccattagact tctttccatc ataggaactg tttctttaac
    24361 tgtgcatatt atttgaccca tatactctgc taaatcctca aattctaaat cctcatagtt
    24421 tttagattta acaccctttc ctaataattc ttcttttgat ttaacctttt ttgataaata
    24481 cttcatagct attgcactac aatcatatga ggtagatgaa gttgaatcta ttaagtactc
    24541 agctattgtt atatcaaaat acatattttc taagtttata ttatatggtt ttaaagatat
    24601 ataatcatct tttaaattat atccatactt tttaatttca ttatttgaaa atatactatc
    24661 taactttgac acttcatttg aatctacata atatattttt tctccatcta cacttataaa
    24721 aatatatatt atattttttt caagtatatt accttgtcct ctaacagttt ttaaaataac
    24781 ttgtcctttt ttgtttactt catctataaa tttttctata tcttttaatt ttaatatttc
    24841 tattttttct tctacgtgtt ctgtgtcatc tgaatcgacc aaatcaacta atcttgataa
    24901 gagacttgta aatccaaagt atctaaattt atctaaaagt tcatctttat cataatcgcc
    24961 aaaaatcata ctatctaaat ccacttctat tggcacatct cttataattg ttgcaagtct
    25021 tttactttga atagctattt ctttgttttc ctctattttc ttttttacac tacctttaag
    25081 cttatctgta ttctctatta gattctctat actagaaaac tcttttataa gctttatacc
    25141 tgttttttca cctattccag gtactccagg tatattatca gacttgtctc ccataagacc
    25201 ctttaaatcg ataaattgtg ttggagtcat ctcgtatttt tcaattacag aattaaagtt
    25261 gtattcttca acttctccaa ctcctttttt tgttataaga gtagttgtat tgtctgaagc
    25321 aagttgaata gcatctttgt ctccagttac tatgtatacc ttatatccat tttcttctgc
    25381 ttttttagaa acagtaccta taatatcatc tgcctcatag ccatctattt ccaatctatt
    25441 tattctaaat ttatctaaaa ggtcttttaa tggctgtaat tgttctgcca gttcatctgg
    25501 cattcccttt cttccagcct tatactcttt atattctaaa tgtctaaatg taggtgcttt
    25561 tctgtcaaat gctacactta tatgtgttgg tttatatata tctatcattt taaatagcat
    25621 tgttgtaaat ccatatattg cattggtctt tagcccttct ttattattca tttctggaag
    25681 agcataaaaa gctctattta ttattgaatt tccatctatt ataattaatt ttttttccaa
    25741 attatatcac tcctaaaaat tatctatttt tgtaacatat atattatata ccaatatact
    25801 gttatttaat attcccataa taataatagt atattaaaat taaatatgtt gcaaacaata
    25861 tatttttttg ctataataat atacatgaca tgcccgagtg gtggaattgg tagacgcagt
    25921 ggactcaaaa tccaccggac taatactccg tgccggttcg agtccggcct cgggcaccat
    25981 taaaaactcc taaagctatt tattcaatag attaggagtt tttttatttt tgatttaaat
    26041 tttaagttat tttttgaaat tactgtgtct ttttaaaatt tatataatta tataaattta
    26101 tcttctagca ttaatttaaa aactaattta cttttatata attttctatt ttatatcata
    26161 acttattaca accttttatc taatttattg tatacaacaa cactacaaat aaattgaaat
    26221 aaaataagca atccaagaac aactaaaacc cacttgctta ttaccattac agaaaataca
    26281 ctcaatatag aaacaataaa tataagaatt acaagaacat agcacatgat ttgattagtg
    26341 atacgtccag ctttatcacg taacataatt cttctttcat cattcatctc tatcctttga
    26401 ttttttatct tttcttcata atctactgct ctttcaggtt tactccaata catatattga
    26461 agaatcatac atacacctgg acctaaagca gctccagata atccccaaat aaaggcctca
    26521 aatgaaaact cagtaaatgt agccaataaa acaagtaaaa ttccacaaat aaaatatata
    26581 attccaacat ataacatatt ctttttcatt tttactcctc ctcataaata aatatttctt
    26641 caatactcat atcaaagtat ctagctatct taaaggctaa gatgatagaa ggattatacc
    26701 taccattttc taatgaacct atagtttgtc ttgatacttg caaaatagaa gcaagttctt
    26761 cttgttttat gcctctttct tttcttattt cttctaatct atttttcaca tctatccccc
    26821 tttaatatct caactacgga aagtctactt tccataatta tacaactata ttccatttat
    26881 gtaaagctta ctttccatat ttacaaaaaa ataacagact attttcagcc tgtaatttta
    26941 gaatttcatg ttgtttatta attttaatgt tatttattaa ttccatatta tttattaatt
    27001 ttaatttggt ttctgaattg gaatccaaac ttctgtagta taatcaggag cattgatatc
    27061 gccattggga taaagttcaa tatctggtaa attagcatac acatacccag aacttggtaa
    27121 ccactcagta ataattcttt tttgcaattc ctgtattgcc agagcatttg gcattggaga
    27181 aacacattca aatactgccc atgtgcctga tggtattaag tattcatgag tatcttctaa
    27241 tacaggctta tctgttggag cagcaatata ataatctaaa cccttactat ttgtacacac
    27301 actaactcca agcaatccac aaggttcgtt atttattaac tctgaaagtt tatcaattgt
    27361 tccatttttt atgcatttag cccataactt aggaactttc tcaaaacact cttctacaat
    27421 catgttcata ctttctttta taccaacaat acgaaaccct ttcttttcta caatacgata
    27481 ttgcatttct atatttcctt ttacataaat gcttaatgtc atcttaggat atgcttttaa
    27541 aacaacaccc tttgctctag ctgcagatgg agaaatgtta tgtatatttt gaaaagctct
    27601 attaaaagat gttggtgaat cgtaaccata cttctcagat agttctaaaa ttttgatgtt
    27661 actgttctga agttcaaatg cagccttagt catacgtctt cgtctaatat attcagataa
    27721 tggtacttct gcaatatatg aaaacattct ttgaaaatga aaggttgaac aacatgcaat
    27781 ttttgctgct tctgcataat ccactccact ttcgagattt tcttcaatat aagtaatagc
    27841 ttgatttaat ttatttatcc attccatttg atattacctc caagcaaatt atacagttta
    27901 ataaaataat atacctctct attcatgcac aaaattgtaa gcatttaaat tcataagtat
    27961 ttttcaatca atacaataaa tataaaaatt gtattataca ataaaaaagt tcaataacat
    28021 tatattataa aatgttattg aactttttat aaaaattaat ttaagtgtag ttaaattaag
    28081 tattacacta tcttatatat tacaatatcc tactatctta tagtcttctg gcactttata
    28141 atctttgtct ggagtaccta aattccattt taaatcaaac atagttgcac ttattattgt
    28201 tgcaaaataa agcataacta taccaatgtc tattttttct tcatataaat tagtatgacc
    28261 atcttttcta acagctaaaa ctacagtgcc tccatcaact ataaatctcc atggttgtct
    28321 atttaatgta gatggagcta atcttgcata gtgaaatgca tcaagaagtg ctctttcttc
    28381 taagatttct atacttgcaa catttcccca ttcgttcata taaacaacat cttctacacc
    28441 tagtctagta gaccccttat ctgttacaac ttgcatatca gattttgaat aattttgccc
    28501 agtttttgta gcatttaata ctttagcatt acttacattt tctccatatc caatagctat
    28561 aattcctgtt acctctttat ctgaattaat tccaagtttt tcttttacct tttttccatc
    28621 ttcaaaagta acccaacaag aaccaattcc aagttctgtt actttaaaga ttatactttc
    28681 tcctatgtac cctgaatttt caatgtaatt atcttttatg tcagataata ttataatata
    28741 acttggagaa tctatcatat ttcctttgta ccctgctact ttatctaaat tattaaaaac
    28801 ttcacttcta ttcataattc ttacatcaac atctatttct ggaactagtt tcttacaaga
    28861 ttctgagtat ttcttaattt cactaagata ttcttcttta atatctgtgt tcttatattc
    28921 tctaacagac tttctattta atatgattgt tttataatcc ataattttat cctccccaaa
    28981 ctagtattgt cttaattata tacccattaa tatattattt tactcattta tttaataaat
    29041 aaagattaca attttgtaat tattatttat taataatatt ccacttttac attaataatg
    29101 accagttgtg caaaatccat tgccaaccac caaataaaaa catattataa aggtaatata
    29161 aaataacaaa caaatcttaa caaaaaatta aacttttttt acacgaaaga aggcttcccg
    29221 ttgccttctt tatttttata aaaaaataag ctgtttctaa aatgatttca catttttgaa
    29281 acagccaagt tattctataa tgacaactat aatttgagga tgtgagggag agttgtcatt
    29341 ataaatttag ggtaattata ttatgtttta agctagttct ttctatattt atataataca
    29401 ttttatatat gaattttata tgagattggt ataaatgata tatgaaatta catatgttta
    29461 tattatatta aattttatta ttacattttc cacaacaata ctcatatgtc tccacattat
    29521 ttggaatatt tacaaataca aattctttac agttctcaca atactttaat atagatatag
    29581 ttccacaagt gtaacattca aatattacac aatcctcatt ttcctctact atttcaaact
    29641 ttttactatg ttcaccacac tctgggcact tgtgattatt tacattcata aatactcctc
    29701 cttattagta tagttacatg tcaaaaactt aattaatgta ttctatatta cttattatat
    29761 attctataat aaatcatatt ctaatataat aaaacaaatt aaataaaaaa aaaccataaa
    29821 ttttaaattt atggattaat tcttgtaatg ataactctat ttgaggatgt gagggagggt
    29881 tatcattaca atagggtatt atattatggt ttttaagcta gtctttctac acctcatatg
    29941 atacatatgc tatatgaatt ttatatgatt tttacataaa tattttatga aatttaaatt
    30001 ttttagtcat tttgaaattt gtaaaaagtt ctctaaaaat ccacagcatc ataaacagcc
    30061 aaaatttaag taaaacatca taaaaaaatt tgtccaataa ttttaacaag taatctcacc
    30121 tcattataat tattgacaca aacttaaata aacaatctat aaatcttcta taaatcttaa
    30181 actttccccc tattgataca caatttctag tttgtcaatt ttattttgca tttctaagaa
    30241 aatttcttca acagaattgt taaatatatt gcctaaatag attcttttat ctttttgaaa
    30301 tgaataagat tttacactac catcagaaga aatatataaa tgactccact ctgctccata
    30361 ttttgaaaac ctctcctttg tatctttaaa cctcatatct tctattttta ttgctttttc
    30421 tctgtctcct cttactacag tgtgacccct ttttattaaa ctggttattt ttatatcttc
    30481 ttttaattta atacgtgtat catcattttt tgaatatact ttccatatca ctttatgacg
    30541 ataattatct aattccttta ttaagcttct atcaatagta aaactatctt tagttaccac
    30601 aattaaatca aaatggtcta acatatattg aaatatttct ctcatacttt tattttttaa
    30661 aatatcctca ccaataacct ctataaatct acagcctttc tcatacatgc agttcaataa
    30721 acttgtgatt tcagaaaaat aatcatttgt attattatct ccatctattt ctatagtaaa
    30781 gtacaaagga gtaatgtatg atttatttcc tgttatctca aatacacttg atagtgtctg
    30841 tatatcatct tcaaacgtta agtatacatt ctcataaaac ataaaaaatt ccataacttt
    30901 ttctttcata ttatggatat ttctatatct attggataat atatttatta tatcgtcagt
    30961 agtatttttc ccatcacatt cagccagtat aacaatttgc ttccaactta agctatccac
    31021 atttccgata ctttttttat atccatcttc ttcatcataa atagggccaa ttataatgtt
    31081 ttcttttggc cacagatgaa ccctagttcc atcttttaat ttcggatatt tattttttac
    31141 agactcataa aatctacttg tatcacccat gattctttac taccccccat agtttaaata
    31201 ttctatgcta aaaatagcac tttattttta ttaattcaac aattattcaa aattggtcat
    31261 tatatatgtt taaatggatt taacatcaac cttatttata tgttattgat gtttatatat
    31321 gttatataaa atcttatttc tcatacttca atgtaatctt aactatctct ggtttacaat
    31381 taagcctaat taaaactttt gagcctccta agcctttatt taaaatcata gtgctattat
    31441 ctttttcata ttttccccag tcatacttag gaaagaactc tctatttgga gataatacac
    31501 ctccaacaaa aggtattctt attatacctc catgaacatg tcctgctaaa accaaatctg
    31561 ctccccattt tgcatatttt tcaaagaaaa atggtgtatg tgctaaaaga atgttatatt
    31621 cattttcatt aactttacct agattgtctt ctacaaaatt gtcacttagt tctaattttt
    31681 tgttattagc aaataaatat ggatagcatt ccaatggtat aattaaacca tataaattaa
    31741 gatggctatt acctctatat attcttacag attcattttc catatttatt attttctttg
    31801 aatatagttc cttaaaataa tccttatata gatgtttata cttttttatt aaagattttt
    31861 gttcatgatt tccaattata tgatatactt catatttttc aaccaaatta tctattaaat
    31921 ctaatgcaac ttgaaaattt ttattttctc catctactaa atctcctgtt ataaatataa
    31981 catctgggtt taatgattca attttatcta tcaatacctt attattttta tcaaacacct
    32041 tattatgtaa atcactcact tgaactattt taaagttatc aaattccttt ggaatttttt
    32101 tgttgaaaat agtatattct gtcgtttcta tatgctgagt ttcatactta tacagaatat
    32161 acaataatat taatactaat actgtcattg aaacctttat aatcatattc tcttcctctt
    32221 tattctattt tgttaatatt attataatgc ttttatgttt tttttaaaac aataacatca
    32281 tcacatacat ataaatcttt tcgacattat aaaaataagt tatccttttc taaaattttc
    32341 atttatttta gaaaaggata actataaaat tatctataaa cctctatatt gtgaaatata
    32401 gtttttcctt tatttaaatt tttatatata tgctctaaag ttccatcaaa cctaatagaa
    32461 tgtccagact ctaaaatata agttttccca tccacattaa ggtcaagttt accttctatt
    32521 acgagtacat actcttctaa aacatgacta tgtgtagatg aaacatgagt acagttttcc
    32581 tcaagctcaa ttataaaaag ttcaaacctt ctttgagggc taaaagggta tattggatat
    32641 aacttcatgt tccctttttg ctcaacaata ggctctactt cttctttctt tataacctca
    32701 taggtatttg tattttcatt taatagtgta gtaaaattta ttttaaatcc agatgaaatt
    32761 ttccataaag tagatactgt cggacttgat tcacctctct caatctgtcc aagcattgct
    32821 ttactgacac ctgtcatctt tgaagctttg tctaaactta tgcccatttc ttgtctcaat
    32881 aaccttaaat tttctcccac attaggaaat ttattatcca tgttaaaatc ctcctgatag
    32941 ctatttgtat tctatgattt ttactcctgt aagttctact atctctttta attttaacat
    33001 atgattgtta caaatttcat caaattcata tatataatct tcaagcccta atgccttata
    33061 tttatcgatt ccaaatttat gatatggaag taactccatc ttgcctcctg gaacattttg
    33121 tttaacaaat aatgcagtat ttcttatatt ttcttcacta tcatttactc cataaataat
    33181 tggcactctt ataaccatag atttattcaa ctttgaaagc ctacatatat tatctaatat
    33241 aattttatta cttactcctg tatattcttt atgaatatta tcatccatag attttatatc
    33301 tacaaatatg tggtctattt tctcaaatac atcatttacc ttattccaat caaaatatcc
    33361 acaagtttca attgcaatat ttatgccttt atcataaaaa atatcaacta attctcttaa
    33421 aaaatcaatc tgaagtgtag gttcacctcc agaaaaagtt atccctccat tagattcaaa
    33481 gaaaaatata aaatcttttt caacttcctc tactatttct tcaacactca tttcttcggt
    33541 cattatacaa atggcatctt taagacatac cttgatacac tctccacata aatcacattt
    33601 attatttatt tgcgttctat caaatagtga acttatattt tgaggacaaa catctataca
    33661 tctattacag gatacacatt tatctttcat aacacctaat tttactatgt tactccagga
    33721 atctggatta gaacaccact tacattttaa tttacatcct tctaaaaata tagtagtcct
    33781 tattccatct ccatcattta ctgaaaaatg ttgtactttt acaactctac cttttttact
    33841 cataatctac ctcataatta aaaattgcta tgtgaatttc tagctattat agcatcttgc
    33901 atttctcttg ataaattaac aaattgagta ctatatccag caaccctcac taataaatct
    33961 ttatattctt ctggattttc ttgcgctttt aatagagtat catttgatat cgtattaaat
    34021 tgtacatgga atcctcctaa agcaaaatat gatttaatca tagatgttag atttctaagt
    34081 cctctttcag tttccaccaa atcttgattc agtcttaaat ttagaagtgt tccaccagta
    34141 tgaacatcat gatttatttt tgatgcagat ttcattgctg ctagtggact tgttgtatca
    34201 gtaccaacaa atggagaaac tccctctgtc aaaggttttg tcgcttttct tccacaagga
    34261 gttgcaccta ctatttttcc agttggtaca tagtttgata tacccataaa tgcactatta
    34321 aatttagagc caaaaatatc cttgtatttt ctagtttcag tataataaaa atcagaaact
    34381 tcaattgcta atgaatctac ataatcatta tcatttccat acttaggaac atccaacgca
    34441 agttttctta attcttcata accttcccaa tcattattta gtgcttttgt taattcttct
    34501 aacgtagtca ccttatcctc aaatactaat ttttttatag ctgccaatga atttgaaact
    34561 acacctaacc ctataccagt taatacagga cctatattat attttgctcc tcctttactt
    34621 aaatcaactc ccttatccaa acatccatct acacaagtag ataaaaatgg tcttggtacc
    34681 atttctttat gaatttgttg agcaacagta gttcctataa cagaactttt tacaagatat
    34741 gctaattgct ttaaaaactc atccttaagt tcttcaaaag aagtaaattc tgtgatattt
    34801 tttgtatcta accccatttt ttctccagtt aatctactct taccttcatt catagcatat
    34861 tctaaagctg ctgcaaaatt gatatttacg gctgaagtcc actgtcctgt ttttctaaaa
    34921 tgaggaacta cacaaccaca gttactccaa tctctagcat cttctgcatc atatccatct
    34981 tgtaataaca tttgtgcccc tgcactatcg ctgtgtatag cagggaaacc agttccttgt
    35041 tttaccaatt tagcaactgc cattacgaaa ttatctggtg ctccttgact cactctaaca
    35101 cttaatcctg gttggtgagt ttttacactt tctgtagcct ttaaacacat atatgagata
    35161 tcattcgttc catcagtacc atttctcttt ttaccaccaa cagtaagatt ttggaattga
    35221 ttatatcctg caaagtaatt tgctgtattt gctgatatag tccataccca ttctgataac
    35281 ttaatccaaa gtgcttctat caattcttga gcttctgatt cagttatttt accttctcta
    35341 gcatcatttt cataatatgg atacatatat tggtcaaatc tacctaaatt taatgccaat
    35401 ggattttctg atagaatacc acctaattga acaaaccata caaattgtat tgcttcataa
    35461 aaattagttg gagggtttgc tggtacattc atacatatct ctgaaatttt tataagttct
    35521 aactttctct tttcatctgt ttcttgctta ctcatctctt ttgcaagatt tgaatatctt
    35581 tgtgccaatg tcataatacc ctgtgcaact atagaaacag atttgtaaaa atctatcttt
    35641 tcaatatttt ctgaaactga tatatctaat tctttaattt ttttatctgc atcttcctta
    35701 atccctttat atccttttac aaacaatata tcttgataat ctggtgttac ttcaccaact
    35761 gcctgtctcc acttagaatc attatctatt attcctgtat ctactaatat cttagctgta
    35821 tcttctggga ttcttgctaa gaaaacttct tccaaagatt ttcccttcca atatggaaat
    35881 atttcttttc ttataaattc tatttgttct tttgatatta catatgggtc ttgagttctt
    35941 ttatcaaaag tatccatttc tttgtctacc cattgccaag aaaactctgg tgtcaatatc
    36001 cctgttcttc taaattttcc tgctgtacct actattaatt cattttcaaa gatttttata
    36061 ggaagttctt tacatgtctc caaaaaagct ttagctcttc ttatacagat agcttctccc
    36121 tcagtttttt tatgtgattc tgtaaaaatt ctagcccttt cacaacatat cataggttta
    36181 gaattaatat attcttgtct aaatgcttct attctattag tttgactcat ttaaaatccc
    36241 cctttggata atatatacta catttttgta tgttataaca tactttaatt atattatact
    36301 gtcctttagc ataaaatgga agcaaaataa cttatttttt ttcatctacc ttgaacacct
    36361 atatattaat actttttttg ttaaatttat aaaatctaaa aaaattttct atgatatatt
    36421 ttctatattg ttatagttat gtctaatata ctaatgattt gaaatatcaa atttgtatac
    36481 tagacttagt tttaaaacct tagttgattg tttaactttg ttaacttcaa catgtatatc
    36541 cttcatataa ggaagctcta caacacctac tcccttatta aagtcttctt caaccataga
    36601 aaaaatttct ttctcagtta agttcgtatt agtcaatgat ttaaataaat tgtatataaa
    36661 tttaatatat ttatcactct cagataactt atcatcttct ggatactctt tagcacagag
    36721 aatatctatt gttacttttc cttctttatc gtcttcagac atagaataga ataaacttcg
    36781 aatatttttt tcaggtggtt tttcagcacc atttatagat actacaaagc taccatctga
    36841 ttttgagtta gtaatactat aaccactgtc tattatagct ttcgaaaatc tttgagataa
    36901 atcttttttt acttcttctc tacctttcgc atcattaatc ttttgtgagt aattattaaa
    36961 tagctcgata gattcatctt ttttatcttc aacttgttct ttcaatgaat ctttactaat
    37021 ttgttctgag ctacttaagt tcttatcaga tacaggagac atacacccta ccatcaatgc
    37081 taagctaaga tttaaagcca caattattgc cactctattt atattccttt ttactttcat
    37141 acacaatcct cccatcacac taaccgctct aaagtattat tcattgcact agaatatatt
    37201 tatattttct ttatattaaa gatttattat tcaatacata tcaccttcct atccaattct
    37261 tataacttac tattagtaaa atattataag ctccttaata cagaaataag gatgttatct
    37321 tttgttatta aaagataaca tccttatttt tttgtatcaa tacatatgtc ccaactggta
    37381 acttaatcgc cccaactgtc agtatcttgg tccaatggta tatatatatt tagtataaat
    37441 ttatcgatac tattttcaaa taataattct ccattatatt tttgtaaaga agatttaata
    37501 ctctgaatac ctattccatg tacgaactta tccattttgt ttgttaacaa tatattttta
    37561 ttaaaagtaa gctgatttag tttactattt tcacatttaa gtacaaaaaa tgaccttgtt
    37621 atagttcctt taattctaat atatttatta acattttcat ccactatttt atcacatgct
    37681 tctattgcat tatccaaaat attagaaaat atgcttgaca catctatagg ttttacaaaa
    37741 tttacttttg aaaaatttat agagcaagta aatttaattc ctttttttaa acatatttca
    37801 ctcttttcgt tgattataat gtctaatatc atatttcctg tattatagat atatttaaat
    37861 gtttttattt catcttttaa attgttaaca tattcattaa tttctttgct attgtttcta
    37921 aggttgtcta tacaataaat gtgattattt atatcgtgat atagtttctt aactcgcata
    37981 tgagattctt gaatacttaa ataatgcgca tattgcatat ctaacttatt ttttattaat
    38041 tcagcttgag attttaatct attctctttt attatttctt taaacaatag tataagaaat
    38101 atactactag ctcctaccaa ttttggatta acaaaattat caaaatagta tccttctgaa
    38161 tgtaatgacc caaaccgttc taattttttt aatacataat ttacaagcat attcattgta
    38221 ttaactaata aggcaaataa taaatataca taatttctct tatcttggct aattaatttt
    38281 atagactttc taaaaaa
//
LOCUS       DAENCV010000032        37634 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAENCV010000032 DAENCV010000000
VERSION     DAENCV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.032"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            63..1607
                     /locus_tag="KQD82_001529"
     CDS             63..1607
                     /locus_tag="KQD82_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG4380004.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLIRG"
     gene            1783..2151
                     /locus_tag="KQD82_001530"
     CDS             1783..2151
                     /locus_tag="KQD82_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4380005.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            2153..2878
                     /locus_tag="KQD82_001531"
     CDS             2153..2878
                     /locus_tag="KQD82_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380006.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2872..3636
                     /locus_tag="KQD82_001532"
     CDS             2872..3636
                     /locus_tag="KQD82_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380007.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3858..5108
                     /locus_tag="KQD82_001533"
     CDS             3858..5108
                     /locus_tag="KQD82_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4380008.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDTKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5299..6183
                     /locus_tag="KQD82_001534"
     CDS             5299..6183
                     /locus_tag="KQD82_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG4380009.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVKFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6545..7162
                     /locus_tag="KQD82_001535"
     CDS             6545..7162
                     /locus_tag="KQD82_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG4380010.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            7270..9801
                     /locus_tag="KQD82_001536"
     CDS             7270..9801
                     /locus_tag="KQD82_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG4380011.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            10235..10936
                     /locus_tag="KQD82_001537"
     CDS             10235..10936
                     /locus_tag="KQD82_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG4380012.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10986..12068
                     /locus_tag="KQD82_001538"
     CDS             10986..12068
                     /locus_tag="KQD82_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4380013.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            12329..13429
                     /gene="vanG"
                     /locus_tag="KQD82_001539"
     CDS             12329..13429
                     /gene="vanG"
                     /locus_tag="KQD82_001539"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG4380014.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13426..14232
                     /locus_tag="KQD82_001540"
     CDS             13426..14232
                     /locus_tag="KQD82_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG4380015.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            14250..16388
                     /gene="vanT"
                     /locus_tag="KQD82_001541"
     CDS             14250..16388
                     /gene="vanT"
                     /locus_tag="KQD82_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG4380016.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGVKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16702..17322
                     /locus_tag="KQD82_001542"
     CDS             16702..17322
                     /locus_tag="KQD82_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG4380017.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17488..18024)
                     /locus_tag="KQD82_001543"
     CDS             complement(17488..18024)
                     /locus_tag="KQD82_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG4380018.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(18137..18811)
                     /locus_tag="KQD82_001544"
     CDS             complement(18137..18811)
                     /locus_tag="KQD82_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG4380019.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            19014..20888
                     /gene="asnB"
                     /locus_tag="KQD82_001545"
     CDS             19014..20888
                     /gene="asnB"
                     /locus_tag="KQD82_001545"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG4380020.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(20980..22416)
                     /locus_tag="KQD82_001546"
     CDS             complement(20980..22416)
                     /locus_tag="KQD82_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG4380021.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKGSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIVFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            22745..23323
                     /locus_tag="KQD82_001547"
     CDS             22745..23323
                     /locus_tag="KQD82_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4380022.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            23362..23940
                     /locus_tag="KQD82_001548"
     CDS             23362..23940
                     /locus_tag="KQD82_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4380023.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            24026..24655
                     /locus_tag="KQD82_001549"
     CDS             24026..24655
                     /locus_tag="KQD82_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4380024.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            24645..27404
                     /locus_tag="KQD82_001550"
     CDS             24645..27404
                     /locus_tag="KQD82_001550"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG4380025.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGRDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            27491..29053
                     /locus_tag="KQD82_001551"
     CDS             27491..29053
                     /locus_tag="KQD82_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG4380026.1"
                     /translation="MGDSKVFEKIFSNQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            29054..30190
                     /locus_tag="KQD82_001552"
     CDS             29054..30190
                     /locus_tag="KQD82_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG4380027.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            30254..31330
                     /locus_tag="KQD82_001553"
     CDS             30254..31330
                     /locus_tag="KQD82_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG4380028.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFADALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            31308..32546
                     /locus_tag="KQD82_001554"
     CDS             31308..32546
                     /locus_tag="KQD82_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG4380029.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENKYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            32600..33688
                     /locus_tag="KQD82_001555"
     CDS             32600..33688
                     /locus_tag="KQD82_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG4380030.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIKTIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            33716..34507
                     /locus_tag="KQD82_001556"
     CDS             33716..34507
                     /locus_tag="KQD82_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG4380031.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(34622..35176)
                     /locus_tag="KQD82_001557"
     CDS             complement(34622..35176)
                     /locus_tag="KQD82_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG4380032.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            35372..36574
                     /locus_tag="KQD82_001558"
     CDS             35372..36574
                     /locus_tag="KQD82_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG4380033.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            36650..37630
                     /locus_tag="KQD82_001559"
     CDS             36650..37630
                     /locus_tag="KQD82_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG4380034.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKYILK"
BASE COUNT        14563 a         3914 c         6533 g        12624 t
ORIGIN      
        1 aaacttttga gatgtactta tttgctaata aaatagaggt gttttaatgg gaggaaattt
       61 ttatgaggaa tttacaaaaa aaattgatgt tttttttatt tatttgtata attttacaaa
      121 ttacatacac aacaccagtc tattctttat gcaaactcta taaaaacaac tccataaata
      181 actttgagtc tattgtgtct aataatcagc ttattagcaa acatgcaata caaataatac
      241 ttattatggc tataatatgt gtaatactta tattctatat aatatatgat aaattaaatt
      301 ttaaaattaa attgcaaagg atagcatata cagataatct tacaggagca aatactatag
      361 ataaatttgt aattgatgct aacaaaatac tatgtaaaaa tactcaagta aagtatgctt
      421 tattatatat agatattgat aaatttaaat atataaatga tttatttgga tatgaagttg
      481 ggaatgaaat tttgcgtaat ttaacaaaga ttattaagag taatatattt gaagaggaaa
      541 tgtttgcaag aatatctgcg gataatttta ttataattat gaaatatata gaagaagaag
      601 atataacaaa gaggttaaaa actatatttg aagaattaga cttgtttaat aataatcaag
      661 aagaaaaata taagttagtt ttaagttgtg gaatctattt tatacttcca gaagatagag
      721 atatcaactc aatcatagat agggctaata tacctcataa aatggcaaag ggaggacata
      781 aaagttcata tgcattttat gataataaga ttcatgacca agagataaaa gaaaaagaga
      841 tggaaaatac aatgttttca agtctagaaa ataaagaatt tattatatac ttacaaccta
      901 agatagaact taatacagga gagatacaag gttcagaagc acttgttagg tggaaacgtc
      961 ctgataaagg gctaatacct ccaaatgaat ttatcccatt ttttgaaaga aacggttttg
     1021 ttataaattt agatttatat gtgcttgaag aagtatgtat ataccttaga aaatggattg
     1081 atgcaggaat aaatccagtt actgtttcag ttaatgtttc aaggatacat ttatattgca
     1141 ataattttat agaaacatac aaaaatataa ttgataaata caatatacct gcaaagtata
     1201 tagaacttga attaacggag agtatcatat ttgataattt tgatatttta attgatataa
     1261 tgaataattt aaaaaaaata ggatttttaa tatctatgga tgattttggt tcagggtact
     1321 catcattaaa tatgttaaaa gaaattccta tggatatctt aaaacttgac caaaaattta
     1381 ttatggaaac ttataatagt aaaagaagca aaataattgt tactaaagta atagaaatgg
     1441 caaaagagtt gggtatgaaa gtcatatcag aaggagttga gacagaagaa cagtttaaac
     1501 ttttaaaaga agtaaagtgt gatatggcac aaggatattt gtttggaaag ccaatgccaa
     1561 ttgaagaatt tgaacattta attgtatcca acttgataag agggtaatta atctgtaata
     1621 taaagaattt aataaattgt gttaaaaatg gactctaatg tagtgttcat tttttttgta
     1681 aaatttttta tacttttact aaaaagtaat tgacatatac tttgtattat tatattattg
     1741 tattaacaac ttaatacaat aatataataa ggaggcatga acatggaatg ggaacttgat
     1801 aataacaaac ctatatatat acaattagta gaacatttaa agttaaaaat aatttcaggg
     1861 gaaattaaaa taggatctaa attagaaaca gtgagagcac tagcagaaga tgcggaagtt
     1921 aatccaaata caatgcaaaa ggctctaaca gaacttgaaa gacaaggttt agtttatagt
     1981 cagagaacta aaggaagatt tgtaacagat gataaggaaa aaataaaagc tatgaaagag
     2041 gaaatagcta atgtggagat aaatacatta aaagtaacct tagaaaagct tggatatgat
     2101 agagatgaaa tgctaaaact tattacagaa aatctgaaag gagaattata aaatggataa
     2161 aaatataagc aatgtttctc aagatggaat agttgaattt aaaggtgtaa acaagagtta
     2221 tggaactaag aatgttttaa aaaatattga tttaaatata ccaaagggaa aaatagtcgg
     2281 gctgttagga cctaatggaa gtggcaaaag tacaatgatt aagctaatga atggattatt
     2341 acagcctgat aatggtgaaa ttatgataaa tggaatgaaa ccatccatag atactaaaaa
     2401 aatagtttca tatttaccag aaagaactta tttaaatgat tggatgaagg tatcagattt
     2461 acttaagttt ttctatgatt tttatagtga ttttgatgtt agaagagcaa atgagatgat
     2521 taaaagctta gatattgatg taaatgaaaa gctaaagaca atgtctaaag gaactaaaga
     2581 aaaagttcaa cttattttag taatgtcaag aaatgcgagc atatatatat tggatgaacc
     2641 tataggtggt gttgacccag ctgcaaggtc atatatactt aagactatat taaaaaatta
     2701 ttctgaagat agtactttac tgattgctac ccacttaata agtgaaatag aaaacatatg
     2761 tgatgagata atattcatat caaaaggaga aatagtgtta caaggagacg ttgaagcaat
     2821 aagagaagaa aaaggaaaat ctatagatgc attatttagg gaggaattta aatgttaggt
     2881 aaattattaa aatatgagtt aaaagcaagt ggaagaatat ttataccgtt atatatagcc
     2941 atattgatag tagctgtatt taatggaata tttatgaata caaatatact tcaggtacag
     3001 ggaataggaa tactagtatt aacaagtctt tttatggcat taggagtttt aactattgta
     3061 gttacaattc aaaggtttag aaaaaatctt ttgggggatg aaggttattt aatgtttact
     3121 cttcctgtta gcactagttc acttatatta tctaaatgta taacagcact tatatacgct
     3181 gtacttagtt ttatagttgc agtattcact tttggagttc ttatgctttt tggtacttct
     3241 ggaattttac tacctgaaat tttagatttg tttaacacta gttttaaatg gatttctgaa
     3301 aactttttag atattttatt attagtagta gtaatgttta tttcatattc atcatttatt
     3361 ttgttattat atacatcaat ctcaatggga caattgccaa aattcaataa acatagaaac
     3421 atagtggcat ttgcttcttt tatagctata aatatagtaa tatcaattgt tggagatgca
     3481 gttggaagca tcttaccaaa tgaagataca aatatggtat atcatcttta tcagagtcct
     3541 agttttatgc ttgcaatact tggtagtctt gtagttgcaa tagcgttatt tttcgctaca
     3601 aaatttatat tagataaaaa attaaattta gaataaatgt ttttatatgg agctatcttt
     3661 tgagtaatgt taaaagatgg ctcttattat ttatgtagtt aattcatgta tataatatga
     3721 atagtgtttt atttctagtt aaatatatac atatgttgta taatgatatt gcgtgtaaaa
     3781 atttgtaaga ataactatac actttatata agttatatca agaaaattta aaaatatatg
     3841 gatttgaggg agaagatatg aaaagttata caaaggctaa gtggagtgtt tggggtataa
     3901 ttacattttc atttgtttta gtattatttt taaggatgtc aactgctgtt gtttcagaca
     3961 atttagcaaa tgagttaggt tttaattcta tacaaatttc aaatatagca tccttttgcc
     4021 tatatgcata tgcatttatg caaatacctg caggaatttt gattgataaa tatggagcaa
     4081 gaaaaattag cagtcttgga ataataatgg caagtttagg gtcaatttta tttggtttga
     4141 ttcaaagtat agaattagca tatatttctc gtgtaattgt aggggcagga acatcagtaa
     4201 tattactttg tattttaaaa attcaaggga gatggtttaa taagtcagaa tttgcttctg
     4261 caacagctaa attttcattt gtaggaaatt taggtggagt tcttgctact tttccacttg
     4321 tgtttttgtc tgaacttgtt ggttggagaa attcattttt attaatcggt ataattggtg
     4381 ttgtaattgg atgttttatg tatattattg ttagagatac accaaaagag tatggattca
     4441 atgtagatac tgaaccctat gaaaagtcag agaaggttaa tattgttgat ggtattaagt
     4501 ctgttataaa gaacaaatca acatggtata attcaatgat aatgttctct tttgttggtc
     4561 ttacaagtgc atttataagc ttgtggggag taagatacat aatggacgtt tatggtgtga
     4621 gtaagagttt ttctgcgttt atagtatcat tttttacata tggatttata tttggttcaa
     4681 taattatgga ttttgtattt gctaaaataa ggtctagcaa atttaatata ataaaatttg
     4741 gagcaatgat agatttgttt atatggattg taattgtagt tgtataccag gtaaaaccac
     4801 caattatagt tttaccaata tcattcttta ttatgggatg tatagttatg tcacatctac
     4861 aggtttttaa tgataccaag tataaaaata aagaaattta ttctggactg gcaactagtg
     4921 ttattaatac ttttgaattt ataggaagtg gaattattaa tttgattata gctatctctt
     4981 tacaagtaaa ttcatataat atagttgatg gatataaaaa aggttttgta gtatttatag
     5041 tactaagtat tataactata gtatcatctc atataggggt aaaaaatgat gattttaagg
     5101 ctatatagtg ttttattaat agtttaattt agtattatat tttgcatata tataacaaat
     5161 agtttgcatt ttcctaatat gtataattgg aatataaatc ttgtttacta tgtacaaatg
     5221 gtattgtatt atattaaata gaaaataaga actttaaact ataatattgg ttctaaaata
     5281 taaggtaagg agttttttat ggatacgacg aaaatagatg atataagcaa gttagattta
     5341 aataatttag atattaataa gatggatatg ggaggtataa tggaaagtct gctagaatgg
     5401 gctactacaa gcggagtaaa acttataata ggcatactta tactttctat tggatttaaa
     5461 ataataaaaa aatttgtaaa ccatgttatg ttattactta gtaaaagaga tattgattta
     5521 actttaagaa agtttttaaa gtctcttctt ttgagtgtac ttaaagttgc cgttataatc
     5581 attgttctgg aatattgggg aatgagtttg tccagttttg ctgctgtgat agcatcagca
     5641 ggggtagcaa taggtttggc tttacaaggt agtctatcaa attttgctgg aggatttatt
     5701 atactattga ttagaccttt taaagtagga gattacgtag aagcagcagg tcatggagga
     5761 acggttgaac aaataggtct tttttataca caattggtta ctccagataa taaacagata
     5821 cttataccta atggttctgt atcaaatgat agtctaataa actattcagc aaagaatact
     5881 agacgagttg atttgacttt tagtgtaggg tatgaagatg atatactaca tgttagaaga
     5941 gtacttaaag atattgttaa taatcataaa ttaataataa atgaaccaga accatttata
     6001 ggagtagtag aacatggaga taatgctatt aaatttgcaa ctagagtttg gtgcaagaca
     6061 gaagattatt ggacaattta tcttgattta ctagaagagg ttaaagttaa gtttgacgaa
     6121 gaaggaatta cgattccata tcctaaaatg gatttaacgg taaaagaatt aaacaaaatt
     6181 taatttttta atatagtgtt taaatttaag aatatcatat agatatactt ataaggtttg
     6241 taaaaaatta ataagtactg ttattttgta agtaaaaata taaaaaaatg ggagtgtctc
     6301 aaaatgaact ttttagttca tgaggcactc tttttgtacg aaataaaata tatttttatt
     6361 atttcaacaa tgaaaaagag gcttgtttcc attttgagac agtataattt ttatgtaact
     6421 ttaattttag taaatataaa gttttaaaat tgataaaata ggaggaattt ttaatataaa
     6481 agggtaaata aagggtttag agggcataat atagaataaa ttaatatatt taaggagggt
     6541 ttttatgtgt aaaaaaacgt atttattagt ggtaagtatg cttatagcgg gtttattaac
     6601 tgcatgtagt gggagtccaa ctaaggattc aggacaagca caagataata caaaaaagga
     6661 ggcaaatgca tcaaataacg ctttaaaaga tgaaaaaaat aatgaaaatt taatggaaca
     6721 agattttaaa gtgccataca cagatgcaat aaacatattt aaagataaat ataaggatgc
     6781 tgatatagta gatttaagtc ttgagagaga tttaaataaa tttgtttata ctgtggaagg
     6841 tgtagatgat aataatgaat ataagatgaa aattgatgct aatacaaaag atgtattaga
     6901 agataaaaca gaaaagttgg attcagaaga tttaaatgga gttgctagaa aagaaaaatt
     6961 agatttaaat gacataatga cacctcaaca agctatggaa attgcattaa aagaacaaaa
     7021 tggaatagtt aaagaatgga gtttagacaa agatttagat gttacttttt ataaaataag
     7081 aatagataaa gataaaaatg aatatgacat aaaagtagat tctaaaaagg gaactgtatt
     7141 gaaggtagaa aaagaagatt agaagattta aactatcttt aaaataattt cttttttgaa
     7201 taaattttgt ttaaaatata acaagcaggg tataaagaga ctaaataact attttaggag
     7261 gagtacggta tgtcattgaa atcactaaaa ataaaagaaa atttatattg ggtaggttcg
     7321 cttgaccctg atttaagagt atttgacata attatgtata caccttatgg aactacttat
     7381 aattcatatg tattaaaagg aacagaaaaa acagttttat ttgagacagt taaagataag
     7441 cattttgaca attatattga aagattaaat gatttaaata tagattttga aaaaatagac
     7501 tatattgttg taagtcatac tgaaccagac catgcaggaa gcgttgaaaa gcttttggat
     7561 ttagctaaaa atgcaaaagt agtagcttca gaaacagcaa ttaaatatct taaggaaatc
     7621 gtaaacaaag actttgaata tgttgcagtt acagatggag atacattatc aattggggac
     7681 aaaactttag aatttttctc tgtacctatg cttcattggc cagataccat atacacatat
     7741 ataaaagagg ataaaactct tgtaacatgt gattcatttg gtagtcacta tagtaatgat
     7801 aagatagtaa atacacttga tgaaaatgaa gaaaaggatt acttggatgc tttaagatac
     7861 tattatgatt gtataatggg tccatttaaa ccatctatgg taactgctat agaaaagata
     7921 aaagatttag atatagatac tgtatgtcca ggtcatggtc cagtattaac tgaaaatcct
     7981 agaaaaatta tagaccttta ttacaactgg agtgtaaatg aacaaataaa gttagaaaaa
     8041 gaagttacaa tttgttatgt ttctgcacat ggatatacaa agattatggc agaagctata
     8101 aaagcatata ttgaaaaaaa tagcaactat aaagtaaatt tatttgatgt aatagagcat
     8161 aagcaagaag atattttggc aaaaatagca gtatcacaag gtgtattatt tggaacacca
     8221 acaatattag gagatgcgtt aaagcctata tgggatatat taatatcgtt aaacccagtt
     8281 cttcatggtg gaaaagttgc ttcagtattt ggttcatatg gatggagtgg agaaggtata
     8341 gaaaatgcta tggagagaat aagtcagctt agaatgacag cagtaaaacc ttttgctgtt
     8401 aactttaaac catcaaatga agagattgat aaattacatt cttatacagg taaattccta
     8461 gataagttaa attctacttt tggaagtaaa aagaaaacta agaaatttaa atgtgtaatt
     8521 tgtaatgaag tttttgaagg tgatagtgct cctagtgtat gccctgtatg tggagctaag
     8581 gaagaccaat ttatagaagt tgaagaagat gaagttactt ttagaaaaga tactgatgaa
     8641 tatttcgtaa tagtaggaaa tggagcagct ggtttttatg cagcagatgc tataagaaaa
     8701 agaaataaaa cttgtaagat aactatgatt tctaatgaag atgagttgac ttactataga
     8761 cctgcacttt ctgatggtat aaatgaagaa cttggttcag atttctacat ggaagataaa
     8821 gattggtatg ataaaaataa tatagtagta atattaggaa cgaatgtaga taaattagat
     8881 gaagtgaata aaactattat agtgaatgat ggagctatta aatttgataa gcttgtaata
     8941 gctacaggaa gtagaaactt tataccacca attaagggtc atgatttaga aaatgtgttt
     9001 actcttagaa atataaaaga tttatatagt gtaaaagaag cactagaaaa atctaagaaa
     9061 gttgtagtga ttggtggcgg tttattggga cttgaagctg catgggaatt tagactaaaa
     9121 ggattagaag tagtagtagt tgaagccatg gatagcatac tatcaaaaca acttgataaa
     9181 gaaggtagta aaatattaga acaatgtgta agagatacag gaatagatgt aagattgggt
     9241 gtagcagttg atggtattga aggtgatgga aaagctcaaa aagttgtatt taaagatgga
     9301 gatagtgttg actgtgatat ggtagtattt tcaattggtg taagagcaaa cactcaaatg
     9361 gttcaggata catctgtaaa aattgataga ggtattgttg tagataagac attacagaca
     9421 aatattaaag atatatatgc ttgtggagat gttgcacaag tgggaaatat tagtttagca
     9481 atatggccat cttcagtaga aatgggaaaa atagcagggg caaatgcaag tggagataat
     9541 ttgacatttg aaagtgaagt atatccagtt tcattagatg caatgaatgt aaaagtattt
     9601 agtattggta atatacaaaa ttttgataaa gaaatttcat caaaagatga aggtcaaaga
     9661 atatataaaa aattatttat gaaagatggt tcattagttg gagctatact tataaatgat
     9721 ttaagctgta ctgttaaatt gataagatta atatctgaaa aaggtgattt tgaggatatt
     9781 atgaaggctg atatactata atttaaatac attaaaataa aaaggagttg tctcaaaatt
     9841 gatttttttc taatcaaatt ttaaattcaa ctcctttttt gaatttttat ataaaataac
     9901 atggaaattt tagtaatgtc tgtggttaca tacataggtt taattatttt ttatatggat
     9961 tgtctctggt ataataaatt gaattgtgtt tgactgatag atatttttat tgaaagttaa
    10021 tacctgttat gcgataataa ggacgtgtta ggggagtttt tataataaca attaatctca
    10081 gtttttgtgt tccaatggta gcatcaacac attctgatgt agtagttctt ttcttataaa
    10141 aattagttac aaatatgctt gtaattattt gatatgtaaa tttaccaata ttataaatga
    10201 tgacctcgat aataaggaga gtgaaaaata atctatgaac gaaaatattt tgattgtgga
    10261 tgatgaaaaa gaaatagcag atttaattga agtatattta aaaaatgaag gttttacagt
    10321 atacaagttt tatacaggta aggaagctct agaatgtatt gaatctaaaa atttggatat
    10381 ggctatttta gatattatgc ttcctgatat tgatggattt catatttgcc agaaaattcg
    10441 agaaaaatac tattatccta tcattatgtt aacagctaaa attgaagata tagacaagat
    10501 aatgggttta accataggtg ctgatgatta tataacaaag ccatttaacc ctttggaagt
    10561 tgttgcaaga gtaaagacac aattaagaag atatatgcga tataataatt cttatgaaca
    10621 acagtcgata attgttaatg agtatgatat taaaggattg attatcaata aagaaacaca
    10681 taaatgtagt ttatttggga aagaagtagc attaacaccg attgaatttt ctattctttg
    10741 gtatttatgt gaacatcaag gaaaagtggt tccttctgaa gaattgtttg aagctgtatg
    10801 gggagaaaaa tatcttgata ataataacac agttatggct catatagggc gattacgtga
    10861 aaaaattcat gaaccttcta aaaacccgaa atttataaaa actgtatggg gagtaggtta
    10921 tacaattgaa aagtaaaaat attaaagtca atcctgactt tactcaagtg agaaataaaa
    10981 tttttatgag aacagcttta atggcattta tagccattat tttcatatgg attttatatt
    11041 cttttatatt gtatggtaag ttttctatct ggatagtatc attttttgaa aatgcttttc
    11101 gtatggaata taaagctgca ttgaatttat atcagcagat tttccgtaat agcatggagc
    11161 tcttcatgat actagctatg gtgattgtgt tttttataat attccgtgtt tatttaaatt
    11221 ggtttacaaa atattttagt gaaataaatc aaggtattga ctctcttatc aaagaagatg
    11281 ttggagaagt ggctttatct ccagagttat tggcaataga aaagaaaata aactcaatta
    11341 aacatatact tgaacaacgt aaatttgaga cacaaatggc agaacaacgt aagaatgaac
    11401 ttattgttta tcttgcacat gatttaaaaa caccacttac atcagttata gggtatctga
    11461 cactacttcg agatgaaagt cagatttctg aagaactgcg aaaaaaatat ttatctattt
    11521 cgttggataa ggcagagcgt cttgaagatt taattaatga attctttgaa attacaaggt
    11581 ttaatttatc aaatatatca cttgaataca gtacagtaag cttaactcgt atgcttgaac
    11641 aactaacata tgagtttaaa cctactttga tgagtaagaa tttaaaatat acactgaatg
    11701 ttacaccaaa tatgatgata aggtgtgatg ttaataaaat gcaacgtgta tttgataact
    11761 tattacgaaa tgctgtttat tatagttttg aagatactac tattgaaata acagctgtac
    11821 aggaggatga ttatataaag cttaaattta tgaatcatag caatacgatt ccagaggaaa
    11881 aacttgagcg tatttttgaa caattttaca ggttggatac atctcgtaat tcaaacagtg
    11941 gtagagctgg gttgggactt gctatagcta aagaaatagt ggagcttcat aacgggacaa
    12001 taacagctca tagcgagaac aatataatta cctttaaggt tattattccg attgtaggaa
    12061 atttgtaaga atttaagtat aaaaaaatag gaaattcttt aggataaata aataaagagc
    12121 actcttgtta ttgatataat tactgtatca aaacaagagt gcttttattt tgatacagta
    12181 attatatcaa tagaaaaatg tgaaaatata ttatgtggaa cagatgcaat tgtaagtgaa
    12241 ggttgattgg atttgaaata gaaaaagtat tttaacatgt ttctgtagat tgtataaaaa
    12301 taaataaatc aacaaaggag aatcaaagat gaataataaa aaaattgcag ttatatttgg
    12361 tggaaactca acagaatatg atgtatcatt acaatcagct tttgcagtac ttgagaattt
    12421 gaatacggaa aaatatgata ttataccaat tggaattaca agggagggta attggtatca
    12481 ttatattgga gattataata atatccagaa taacacttgg tttgaaaggg atactgatat
    12541 aattccagtt attgtttcgc agaaccgtgt caaaaatggt attattgaat ttttaattcc
    12601 tgatgctgtt tctcagaatc atattccaaa tagtgctatt gaatcatgtg aaaatgatat
    12661 taaatacact aaacttgatt ttgtgtttcc tgtattgcat ggaaaaaatg gagaagatgg
    12721 aacagtgcag gggttatttg aattggcagg tatacaggtt attggatgtg gtactctttc
    12781 ttcagcatta tgtatggaca aagacaaatc acataaactt gttcattcag aaggtattaa
    12841 agtaccaaaa tcaatattac taaaatactc agataaattt aatgtggctg aactgtcaaa
    12901 agacttaact tatccattat ttataaaacc tgtgcgtgca ggttcatcat ttggtattag
    12961 taaaatttat aaaaaagaag agttacaagt agcagtagac ttggcttttg aacatgataa
    13021 tgaagtaata attgaggaaa atattgatgg ttttgaggta gggtgtgcag ttttaggaaa
    13081 caatgactta cttgttggaa gagtagatga aatagaattg tcaaaaggat tttttgatta
    13141 tacagaaaaa tatacattga aaagttcaaa aattcatatg ccagctagaa ttgatttgga
    13201 ttgtgagaaa agaatacaag agacagcaaa aattatttat agaactcttg gatgttctgg
    13261 atttgctaga gtagatatgt ttttaactcc atctggtgaa attattttta atgaagtcaa
    13321 tacaattcct ggctttactt ctcatagtcg ttatccaaat atgatgaaag gtattggact
    13381 gtcttttgaa gaaatattgg acaagataat tgaggtgtac ataaaatgat gaataatatt
    13441 ttattagata aaaataaaga agataaaatg acaaatacta tcttattgac taaagataaa
    13501 gtttatgatg ggaatctaat tctagtaaat gcttttttac cagttaaaac atctgaagat
    13561 atagatttaa ttcctgtaga tacaaggttt ccaagtattc ttatgaaacg tgaagctaca
    13621 aacattttac aaaatatttt aaaaagtata tgtggaataa atgaaatagt acctgtaagt
    13681 ggatatcgta ctgcagagga acaacaagat atatattcaa gttctctgcg tgacaatgga
    13741 aaagacttta ctaaaaagtt tgtagcactt ccaaatcaca gtgaacatca gacagggctt
    13801 gctattgatt tagggttaaa gaaagaagtt atagatttta tctgccctga atttccttat
    13861 aatggtattt gtaatgagtt tcgtaaggca gcactacatt atggttttat agagcgttat
    13921 cagtgtaaaa aagaagaaat aacaggaata tctcaagaac catggcattt tcgttatgtg
    13981 ggatatccgc attctgagat tatcaataaa atgaatctcg ctcttgaaga atatatagaa
    14041 tatattaaga gattttcgta tacaaattgt ttaaaaatag aagataaaga aaaaattata
    14101 gaaatctttt atgtgcctat tcatactaaa gaagaagtga gaatctctat acctgaacaa
    14161 tgtgtatatc aaatatcggg taacaataag gatggtttta ttgtgacatt ttggagaaat
    14221 agaaaggact aagaaatgga ggaacaagaa tgattaggaa taaatcttat gcagggcttg
    14281 attattttag gattatagct gcattactta taattgcaat tcatacatct ccattattat
    14341 catatagtaa aatggctgac tttgtgttaa ctagtataat tgctcgtatt gctgtgccat
    14401 tcttttttat gctatcaggc ttttttctaa tttcaacata taattataat tgtaataaac
    14461 tgaaatcttt tctaaaaaat gtattattta tttacgggat agctattgta atttatattc
    14521 ccttaaatat ttacactgga tatttttcca tgaataattt agtgcccaat attattaagg
    14581 acattgtatt tgatgggact ttatatcatt tatggtatct tcctgcatca attattggtg
    14641 caagtattgc atactttctt gtaaaaaaac taggtttaaa gtactcacta gtaatcacta
    14701 ttgttattta tgtaattggt atgtttggtg atagttatta tggtttttta gaaaaaatac
    14761 cactattaaa gaatttatat gggtatattt ttgaagtatc agattataca cgaaatggca
    14821 tattttttgc acctgtattt ttcatacttg gtggaataat atctaataaa tctaattata
    14881 tgtcaactaa aaatagcttg attggatttc ttatatcatt cttacttatg ttatgtgagg
    14941 gaatgttttt acataaacta ggggtacagc gtcatgatag catgtatatt atgttattgc
    15001 catgtatgta tttcttattt acagccttaa catttttgaa aggttctcga gctaggttat
    15061 tgcgaacatc atcattaatt atttatatta tacatcctat gatgattgtt gttattcgta
    15121 tgttttctaa aatattcgga atacaaactg tcttggttga caatagcctt attcattata
    15181 tagtggtaag tatagcatct tttatagctt ctataattat tatattgatt cttaaacctc
    15241 taaaaaaaga cttgaaagga gaacataagg taaataccaa tagagcttgg attgaaattg
    15301 atttaaataa tttggaacat aatgtaaaga tgatacaaaa agctatgcca gaagattgtg
    15361 aattgatggc agttgtaaaa gctaatgcct atggtcatag tgcttttgaa gtagcgactt
    15421 gtgcaaacca aataggtgta aaagcatttg cagtagcaac aatagatgag gggattgatt
    15481 tacgtcgata tggaatacta ggagaaatat tgattcttgg atatactaac ccaataaggg
    15541 caaaggaact tcataaatac aatcttacac agagtgttat tgaccttaat catgctatct
    15601 ctttaaacaa acagaaatac aacataaaat cacacattaa aattgataca ggtatgcaca
    15661 gactgggttt tgatgttaat gatgtgaggt caattttaaa ggcatttaaa ttaaaatact
    15721 tagatatatg tggcatattt acacacttgt gtgtttcaga tagtctgaaa gatgaagatg
    15781 tagactttac aaataaacaa attgaatgtt tttataagtt aattgatttt ctgttaaaaa
    15841 aaggtgtaaa aattcctaaa gtacatattc aaagtagtta tggttttttt aattatccca
    15901 atcttaagtg taattatgta agaattggaa ttgcattata cgggatatta agttctccaa
    15961 atgatacgac taaattgcaa cttgatttac gacctgtttt atccttaaaa tcacaagtaa
    16021 ttcttattag aaaaatacaa aaagatgata gttttggata tggaagagtc tttattgcaa
    16081 gcagagatag cgtaattgct atattatcta ttggctatgc cgatggtata cctagaaatt
    16141 tatcctgtgg aaaaagttac gtaataataa atggttgtag agcagcaata gtgggtcgaa
    16201 tttgcatgga ccaacttgct gttgatatta cagatatacc taatgtagaa gttggtaata
    16261 cggcaataat aataggaaga gataatttat ctgaattatc tgcttctgaa gttgcaaata
    16321 actcatgtag tatatcaaat gaattattga gcagagtagg aaggaggtta aatgttataa
    16381 agaagtaact caattaaata gtaatatttg ttgtactgtg taatttattt taatctgact
    16441 agacattttt agtaatatta tgtctaatat atatttttta taacttacat gacaatttaa
    16501 gctataaaaa ggtgatgttt cagattttta ttttgaaaca tcaccttttt tgttataata
    16561 tataataaaa taattatacc ttaactgtaa acttatatag attatttact ttttaatatt
    16621 agattcattt tttgagattg gtatataata aacatataga gtatataata atagttaaag
    16681 ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaat tacttgattt gactcaaaat
    16741 tattgggtgt tagccacaat aatcggctta ttaagtgcat ttatagaaag ctttatacca
    16801 gccttaccac ttgttgctat agttacagca aatgctgcaa tacaaggatt atttattgga
    16861 tgtttgttat catggattgg ctcaggactt gggacaactt cattattctt attaataagc
    16921 agatttaatg atagtaagct ttttaataag ttgagaaata gtaaaactga aaaagcaata
    16981 agttggatgg ataaacaggg atttaagtta ttatttatag cgtatgcctg tccatttatg
    17041 cccggttgtt tagttactat cgcatcagct tttggtaaaa aagatttaaa agattttgtg
    17101 ccagcaatgt tggcaggaaa atttgtaatg tttatagtta taagttacgt ggcaagtgat
    17161 atagaaggtt ttataacaag tcctttaaag atagcatcat ttatattatt ggtgtttttg
    17221 tcatggaaga ttggaaatag agtaaataaa aatcttgaaa atcataatta tgatttccat
    17281 cataaaaaac atcacaatga taaagatgat aaaatgattt agcttgtacg aaataaaacg
    17341 tatcttaaaa tgatttagta ctaaattatt ttaagatacg ttttattttt tgtattaagg
    17401 tacaaaattt ttagttcttt tgtaaactaa aatatataaa acttatttat acttagttca
    17461 aaatattgtt atttattttt tctacaacta taataaaata taaaattgat atccaatcaa
    17521 aaaaataact atgaaattta atagcttaga tatgaatttt tctgtttttg agttagtttt
    17581 tataggtagt aaagaaatgt ttatggtaat gggataaaac aattcaatac cttctttagt
    17641 aataaggtca agacacaagt gagatagata tcctattata aacccactgg acaaacatga
    17701 gaaaactata ttattgttag tgaagattgt cagagattta aaaaaactat aaataagtaa
    17761 taaggcaatt agactgtgag taaaccctct atgtctaaac ctacttccaa ataatttgta
    17821 tattaacata aattttttac ttatgtaaga acctctcata tctatatctg gaaataaaga
    17881 acctaaatat gcaaaataaa tataaattat aattaatact attttatatg caatatcgta
    17941 tttaactagg tatagattgt ttataaaggg gagtatcaaa agtgcgaata tataacctcc
    18001 cctttcatgt gtttctttta tcatataaaa atcctcctga atcgtcacta taaacattat
    18061 attggtaaat ttagactatc ttttattata ttatatagca aacataagtt tttttcaaaa
    18121 ggattttaat atttaattaa tcttgagatt ttaaattttt gtaattttct aaagcaccat
    18181 tccaatttac tacattaaac cagttgtcaa tgtattcatt tcttctattt tgatatttta
    18241 aatagtaagc atgctcccaa acatcaagtc ctattatagg agttaggttt ttacttacag
    18301 ggctatcctg atttggagta gtcataatag ataatttccc atctttagta gctacaagcc
    18361 aagcccaacc agaaccaaag acatctaaag cagatttttg gaactcttgc ttaaattttt
    18421 caaaagaacc aaagtctcta tcaatagctt cttttaaaga ttcagaaggt atggtttttt
    18481 ctggtgtcat tatatcaaaa aagaatttat gattataagc tccacctgca ttatttctta
    18541 cagttgtagc aatatcttta ggtaaagaat ctaaattttg caataattca cataaagaat
    18601 aattataaag ctcaggatat ttttcaagag cagcatttaa tttatcaaca taagcttgat
    18661 aatgcttatc atgatgcagt ttcattgttt ctttatctat ataaggttca agtgcatcat
    18721 atgcataagg taatggttta accttaaatt tgttattttc aggtgtaaaa gcaaatgaag
    18781 ttatgcactg tgagattata aataaagaca taataactgg tattaatatt tttttcttca
    18841 taaaaatcac ctcctaatat agtatttcta gaaataaaaa aaatatgaaa tattaacaga
    18901 taatttataa gaatttaata gcaatttcat tttaaattga ataagtttta aagccaaaga
    18961 caaaactatg aaatatagaa taagactaaa ggacagaaag gaattttgat tatatggacg
    19021 gcaatatttc aatttattat aaaggtgata tatctaaaaa agaagatatt aaatttaaaa
    19081 taaaagaatc tgtaaataat acaagacttt ctagtagaat agagtataag gatgaggaaa
    19141 aaacttttga ttttgaaaga tttaatgtaa ttgatgctat ttatggaaat aaaccatttg
    19201 aaaaagatgg aaaagttatt gtttttaatg gaaatatata taactatcat gaaataaaag
    19261 aagaattaat taaaaaagga cacaatttta ctacaaatga tgatattgaa gtcttattgg
    19321 catcttatat agaatttggt aagaattgtg tacataagat taaaggaatg ttcaacttta
    19381 taatctatga tagagaaaat gaaagtatat ttggagcaag agatttattt gggataaaac
    19441 cactctatta tataaataag gaaaatgcaa taattttttc ctcagagtat aagtttttac
    19501 ttgaatatat gaaaaattta aatataaatg aaagaagctt acaaagttat ttttcatttc
    19561 aatatgtact tcctgaagat actatgatac aaggaataag gctgatacca gcaggtcact
    19621 attttagggt agaaaatggt atactatctt taaagagata taataaactt gagtttagaa
    19681 gtagtacaaa atttttttat actaaaaatc acttaggcaa tagagatgtg aacgaggatg
    19741 atataagaaa tatagtagta gattcaatag taactcatat ggaagaggag aaagaaattg
    19801 gaacattttt gtctggtgga atagattcat ctattataac tactgtggct tcacaaatta
    19861 atccaaatat aaaatctttt tctgcaggct ttagtgtcaa aggatatagt gaattagaag
    19921 tagcaaagaa aacagctgat aagttaggta tagaaaatat ccagattaat ataacacaag
    19981 atgaatatat aaaatcatta ccgaatgtaa tttattcttt ggatgaccct atagcagacc
    20041 cttcagaagt aggtctttat ttcttaatta aagaggcagg taaacatgtt aaagtagcat
    20101 tgtctggaga aggagcagat gaattatttg gaggatataa tatatataaa gagtatagta
    20161 ctatgaaatc agttgtcaat tcacctactt atattaaaag tatattagga agagtttcag
    20221 aacttatgcc aaacataaaa ggaagaaatt atctttatag agctacaaca ccattagaaa
    20281 aaagatatat aggaaatgca aaagtttttg aaaacagcga agtaaaaaga ttctttttta
    20341 agtataagga aaagaatata tatgaatacc tgttgtctaa tttgtataga gatgcacaaa
    20401 aaaataatta cgattatata agtaaaatgc aacatataga tgtgaataca tggcttcaag
    20461 gcgatatact tcaaaaaatt agcaaacttt ctacagctga gcaggttgaa cttagagtac
    20521 catttttata taaagatgta tttgatgtag caaaaaattt aagaatggag caaaaaataa
    20581 ataagaataa tacaaaagtt ttacttagag aggcgtttag agaaatagtc ccagaacatg
    20641 ttgttcaaag gaaaaaatta ggatttccaa ctccaataag agtttggtta aaagattctc
    20701 taggggatat tgtaaaggaa actatttata attcaaatgt agatgaattt atagataaaa
    20761 aatatgcaat aaaactttta gatacgcatc ttaaaggtca tagagataat tcgagaaaaa
    20821 tatggactat atttacattt tgtctatggc atcaattatt tattgagcat aaaaatgtcg
    20881 agtattaata cttttttcaa tattaatcag agggcaaaag catgcaatgt tttcaccctc
    20941 ctgattatta tggaatagga taagtagaac tttgattgat tagtttacta tctcaataaa
    21001 attattggca aatattttga caagattact ttcaacagct aaatttaagt tttgtccatc
    21061 atcatatcca gcggaaatca atcctagtaa gtttccaaac atatctaaaa gagctcctcc
    21121 tgaactacca ctagagattg gagctgtaaa ttgtatcatt tgaaccgttt caaaatcacg
    21181 aaaagctgaa ataattccat ctgaaactgt attaaaaaga cctaaagggc ttccaattgc
    21241 aactattttt tgacctctca caggtttttt actgacttta acaggtattg gcttgcattt
    21301 tctattaacc tttatcacag ctaaatcata atctgaatgg tattttataa tttggtaaga
    21361 tgtataaact ttatcatcat tttcaaatct aactaaaaat gaatacccat cattgactac
    21421 atgaagattc gtaagtatat aaccttcatt atttattact actccagaac cgctcttaaa
    21481 cggtttattt tcgttattgc acacaacaat cataacaaca gaagctgata actttgcaag
    21541 gtcttcataa gaaagttcta cttcctcatc ttgtacaatt tttttagatg ttagatttgt
    21601 gttattaagt gtatttttag agttaggttt attcgtagta ggagaagtct tattggtatt
    21661 ttcactgtaa tttatatttg taatatgcct ttcaaaaggt atttctaata tatttggagt
    21721 cttcctagaa agttcaaata gagtagaaaa ttttgaacat tcaggatata aatcaagaat
    21781 atctttattt ggatttatat ataaaatatc acacccaagt tgtgataaaa aatataagaa
    21841 aaaaagttct tgccttttaa tatttccaaa gtatactatc tttggtgaaa tagtagtctt
    21901 caccttgtta taaaataatt taggtagaaa atagtccatc caacacaaaa gtttaactgc
    21961 aaagtttttg atgatagagg atgtacaatt tggcatactt gtggaaaata aatctaaagt
    22021 atttttaaat acaatctttt tagtccaatt taaggtgtca ttttctattt tacatggaaa
    22081 gtcgatatta tataaatccc aactgttaaa cttagaacta tcataaatat taaaaatttt
    22141 aagcatctta tctgtttgtt ctttgtcaaa atttattttt agattgttta atctaatata
    22201 gggaattgta ggattattaa aaaaataagt atctatattt ttaatttttt ctatgtattt
    22261 atcaattgaa tcatcaacac ctatatatct tgtaaagtag acttctttaa tatatgaaga
    22321 acccttattt ttataggtag tatcagatat aaattcaatt agaggttcag tagacttttt
    22381 tattgaaatc tttaatggtt taatagtaca aatcatattt tttctcctat aataagatga
    22441 tataatctat tataaaacaa cttaaaacaa tatagtacaa taattttgta gtttcaaaat
    22501 aaaatatgta cttgtttata taaatgatat ataattacat attaaacata gcttttatac
    22561 atttaataaa gtatgtaaaa tctatgtaaa aagtaagaaa tataaacatt tttttaatta
    22621 tattttaaaa atatcaagca agttttcttc acaaatgaat aaaaaatgtt ataatatgat
    22681 taaagaaaaa gataaataat tagcaagatt tgtttagcta aaattaacaa gggagcgata
    22741 aattatgggt ataacattat caaaaggcca aaaggttgat ttaactaaag gaaatccagg
    22801 tcttaaaaat attttagtag gtttaggatg ggatactaac aagtatgatg gtggatttga
    22861 ctttgattta gatacagcag catttttaac aggagcaagt ggaagtgtta ctaatgatgg
    22921 agattttgta ttttacaaca atttaaaaca tacatctgga gcagttgagc atctaggaga
    22981 taacagaact ggcgaaggtg atggagatga tgaacaaata gttgttgatt tatctaagat
    23041 tcctggagaa ataagcaaga tttcttttac tgttacaatt catgatgcaa cagaaagaag
    23101 acaaaatttt ggacaagtaa gtaattctta tataagaata gtagataaag acacaaatga
    23161 agagttaata aagtatgaat taggagaaga ttttagtata gaaacagcta tagtagtagc
    23221 tgaaatatat aagcacaatg gagaatggaa gtttaatgca ctaggttctg gctttgaagg
    23281 tggtcttgct gcattatgtg gaaactttgg aataaactta taatttcaac tataacaata
    23341 aactaaaggg ggatattttc catggctata gtattaaaaa aaggacaaaa aatcgattta
    23401 actaagggaa atccaggtct taaaaatata aaacttggac taggatggga tacaaattca
    23461 tttgatagtg gctatgatta tgatttagat gtaagtgttt tcatggttgg agaatctcaa
    23521 agagttgaaa aagatgaaga ttttatattc tacaataact taaaacatcc atctggagca
    23581 gttgaacact taggtgataa tagaactggt gaaggtgatg gagatgatga ggaaatatta
    23641 gtgaatttag agacaatgcc aaagcatata caaaagatag ctgtaacagt tacaatatat
    23701 gaggctgctg aaagaagaca aaattttgga caagtaagca actcatatat aagagtatta
    23761 aattctgaaa atgatgaaga aatattaaga tatgacttag gtgaagaatt tagtatagaa
    23821 actgcaatta cagtatgtga gatatataag tataatggag aatggaagtt tagtgcagtt
    23881 ggagcaggat ttgaaggtgg cttagaagca ctttgtagaa actatggctt aaatgtttaa
    23941 tttaagcaat ataatatatt aaatataaat aacctataca aataatgtat aggttatatt
    24001 atataaaatt ttgtggaggt ttagtatgtc gattaattta tcaaaaggtg ataaaataga
    24061 tttaaaaaaa tctaacccag gactgagtaa catacttgta ggtctaggat gggaccctgt
    24121 gcaacaatct ggaggaggtt tttttaaatc gttatttggt ggaggacaag ctgatataga
    24181 ttgtgatgca tctgtattta tgcttaatca agaaggtaaa ttaagtggaa taaaagattt
    24241 aatttatttt ggaaatctaa aaagtgcctg taaatcagtt ttgcatacag gagacaatct
    24301 aactggtgag ggtacaggtg atgatgaaca aatcctagtt aatttggata aagttccatc
    24361 aaatattcat aaattattgt ttgtagtaaa tatatacaat tgtgtagata gaaaacaaca
    24421 ctttggaatg atagaaaatg cctatataag agttgaagac caaggtaata agaaagagat
    24481 agcaaaatac aatctatctg ataattattc tgaaaaaact actcttatag taggagctat
    24541 atatagaaaa gatggaagtt ggcaatttaa agcaattggt gaaggaacaa aagatgcggg
    24601 tcttaaagaa gtaatgcaaa atttagacag gattgagtgt gcatatggaa tttaataatg
    24661 atatcaaata ttataatgta gataaagaag aagttacaaa agacttatct gttaatcctg
    24721 aaaaaggttt atcagaatca gaagtcaaga ctagaagaga aaaatatggt ttgaatgaat
    24781 ttactccaaa agaagagggt agtttttggg atgatttaaa ggaaagttta agtgaaccaa
    24841 tgatagttat cttgattatt gcagcagtag taagtgcagt aattggggaa actcatgatg
    24901 caatagggat tgtaggtgct atagcaatag gtatagcaat aggtatgatt acagaaggtc
    24961 gttctaagaa agcagcagag gctctttcta agttgactga aaacatagaa gtaaaagtat
    25021 tgagagatgg ggaagtacat caaatttcta agagtgaatt ggtaccaggt gatatagtat
    25081 atattgaaac tggtgacatg gtacctgctg atggtagact tatagaaagt ataaacctta
    25141 aaattagaga agatatgtta acaggagaat ctgatgacgt atctaaaaag tgtgatgtag
    25201 tagtttctat ggaagagata gaatctaaag gagcagtagt agtacaggag ccaatacctg
    25261 caaaacagat aaatatggtg tttggtggta cacttgtagc ttatggtaga ggagctctag
    25321 ttgtaacttc aattggagat aatagtgaaa tgggtaaaat tgctcaaaac ttatcagaaa
    25381 cagatgagca aactccactt cagataaaac ttggaaattt aggtggtttg atagccaaag
    25441 tttctagtgc agtagcagga ttactattta tatttatggt tttccaaatg ataagtaaga
    25501 atgtattaaa tgtagatatg tctggaatat taccgttttt agaatcaata gacccagtta
    25561 aaacagcatt tacagtatgt gttgcgttga tagttgcagc agtgccagaa ggtcttccaa
    25621 ctatgataaa tatgacactt gcaataacaa tgcaaaaaat ggctaaaata aatgcattag
    25681 ttaccaaaaa agaagcttgt gaaacgatag gttctgtttc tgtaatatgt tcagataaaa
    25741 ctggtacatt aactgaaaat agaatgactg tagaagttgc atatgttgat ggcagatata
    25801 ttgaaagttc agaagaggaa ataaatagtt attttgaaga aaactgtatg ataaactcta
    25861 cagctgatgt agagcacaat gatggtgata ttaagtattt aggaagtgct actgaatgtg
    25921 cattgctttt atattacaaa aacgtagatt atagacaagt tagaaaaaat tctaatatag
    25981 tagctcaaaa tccttttact tcagattcaa agagaatgac ttctgttata ggtcgagata
    26041 atcatcatgt gctattatca aaaggagcac cagaagtatt acttgaatta tgttcacata
    26101 ttcaaagagg aaaagacata gttccattaa ctgaaaatat aaaacatgag attttagaag
    26161 aaataaagaa attgcaaata aaatcaatga gaactttagg gtttgcatat aaagaaatct
    26221 cacaagctga agaagaagca gcagtaactg ctgaaagtga tgctatgaat gtaagtgcaa
    26281 tggaaaacaa tttagtattt agtggttttg ttggtataag agacccactt agaaaagatg
    26341 ttatagagtc tgtaaataca gcaaataaag ctggtgtttc tgtaaaaatg cttacaggag
    26401 ataatataaa tacagcaaga gctataggtg aagagttagg cctattaaaa aataatatga
    26461 gagtggttga agcttcatat atagatactt tagatgatga agaattaaaa caagagatac
    26521 aaagtatttc aatcgtggca agaagtaaac cagatagtaa aatgagaata gtttcagctc
    26581 tgcaaaagag cggagatgtt gttgcagtaa caggtgatgg tataaatgat gctccagcac
    26641 tttcaaaagc agatgttggt atagcaatgg gtatatctgg tacagaggta tctaaaaacg
    26701 cagcagacat aattttaact gatgatagtt ttagtaccat tgtaaaaggt attaaatggg
    26761 gtagaggaat ttatgataac ttccaaagat tcgttcaatt ccaattaact gtaaatgtaa
    26821 ttgcattttt agtggctata atatctcaag ttatgggaca agagatgcca tttactacaa
    26881 tacaattatt atgggtaaac atcattatgg atggaccacc agcactagca ttaggtttag
    26941 agccagttag ggaccatgtg ttaaacagaa aacctgttga tagaaaagcc aatataattt
    27001 ctaagtcaat ggtgtataca atagtattaa atgcatttta tataacagca atactaatgt
    27061 tacaatctac atttaatttc ttaggagttg atgaagcgca taagggaaca gtaatgtttg
    27121 cgttatttgc ttttagtgca ctgtttaatg catttaactg tagagagttt aatgcagata
    27181 gtatcttccc taattttaca cataacaaat tagcactaca aataatagtg ttgacaggaa
    27241 ttgcacaagt aatatttact cagctattcc aagatttctt taactctgta gcaatggatt
    27301 ttatgacatg ggtaaaagtt ttatgtgtag cagcaagtgt aattgtggtt aatgaagttg
    27361 taaaatgtat aataagactg gtaaaaccaa aaaagactgc ttaaaacaaa ttttttagaa
    27421 gaaaacagac tgactcaaaa tatgaatttt gggtcagttc tgaataaaca tttaaattgg
    27481 gaggatagtt atgggtgact caaaggtatt tgaaaaaata ttctccaacc aatcagatag
    27541 aggaaattac actccttcaa agggatatct aagttatttt atttcgtata ttggtttaga
    27601 agatgaagtt ttgtacaatc tcgagatatt taaaacaaaa caaaatattg atagcaaaaa
    27661 agatattgct ttatttactg atgttatagc aaatcctagt gattttgata ttataaatta
    27721 ttttaaaagt ggacttcaaa aatatagaac tagcatggaa gatgtagata taaatatttt
    27781 gggatttgaa gagattgatt ataaaataaa gcaagcaatg gatagagtgt taaaagaaga
    27841 ggaaaaagaa tttactaatg atagagttaa acaaaatttt atagtgaaaa taatggcatg
    27901 gataaaaatt tatatcggtg ctttagatat aaataaaaat gaagctccaa aagtaatatt
    27961 ttatggagat ataaaaaaac atgaagttta tttactctta atattatact tggcaggttt
    28021 tgatgtttta tatcttaatc caaattcaaa gagtaatata gatattttaa aatcagaaag
    28081 atacaatata gagtttgaag aagccaacat aatagaagaa aaaatttctt ttgaagaaag
    28141 ggttatttta ggggaaaaaa tagataaaag ttctgtaaaa aaagctttta ctgttggggc
    28201 agaagcatca aagcgtataa gtgaggaatt gttaaatgat gcaggattta taaaaccttg
    28261 gcaactccaa gatagaaaaa taaaaaatct acttctaagt tcaactgttg atgaaatatc
    28321 aatatactgg aatcaaccac ttaaattaag accaggattt aagtttaatg atgctattgt
    28381 agaagctcca aattttcttt ctaagattaa tggaatatat aacgataaga atgaatatat
    28441 taagttttta gatttattaa gagattcgga aagttcaaca tttattgaat ttaatggaga
    28501 tgtagataga ttttcaaaag cttttacaag agaagctttt agtttatcct ttttattgga
    28561 ttcaaaaggt gcaatagata aaaactctgt tttaaataat aaagattatt ccatatcaac
    28621 actagcattg aatcaacaaa ttatgatttt agagaaagtg gaagagttgt tagaaggaag
    28681 tatgtttttg aatggtttaa gtggagaaga caagataaaa ggtctattta cagtacttca
    28741 catggataag aaatttgttc atatgatgaa caatttcgat tactcactta taaacccaaa
    28801 attgattata tacatgtaca agagtattgt atttgataaa gaaatagttt ttcttatgct
    28861 tttacttagt aagattggat ttgatattat catattatgt ccaggaggag aaaacaatat
    28921 agagaatgtt ataaataacc aattgattga tgtacacaga ttggataaaa tggtatatga
    28981 ccttaaatta aattcgcttg aaaatgatat acctttactt aaaaaaattt ttggaaagag
    29041 gagacgattt tagatgggga taaatataaa tagtttttca aaagataaga actctgtaga
    29101 ggttactgag aatttgccta cagcagttca agaagaaaac ttcgatataa tggaatatac
    29161 aaataataaa aaaaatgaac tgagaaagtc taaggaagtt gaggcattga catctcttat
    29221 agaagtagaa aatccagata cgattttaca gtttggtagg aaagcatctg aaggagtagc
    29281 aagagtttct gatagccttt taaatacaat aaaattaaat agaaatgaag aaaattctaa
    29341 aatgctggtt catttaacta aaataatgga taagtttgat ttagacgatt ttcaagaaac
    29401 taaggaacca aatttcgttc aaaaactttt caaaaaagct aataatgcca ttgaaatgat
    29461 gtttcaaaag tatgaaacat tgggaggaga agttgagaaa attcaaattg agcttgagca
    29521 atacgaaaga gatatagcac tttctaataa gcaaataggt gctatgttaa atgaaaactt
    29581 tgagttttat aatgaacttc aaaaatatat tgttgctgga gaaatggcaa tagaagaaat
    29641 ggataatgaa atactacctt tctttaaaca aaaatcagag actagtggag atcaaatgga
    29701 tgttgtcaat tatcaagaac tattaaaagt ttatgatatg ttaaatcaaa gagtctatga
    29761 tttaaggata gcagaaaata tagctatcca aacaatacct atgcttagag gtatgcaaca
    29821 taataattat ggtttaatta gaaaaataaa tagtgcattt gtcgtaactt taccagtatt
    29881 taaacaatgt ttatctcaag ctatattact taaaaagcaa gaattacaag caaaatctct
    29941 aaaagcactt gatgacaaaa ctaatgaatt attgcttaga aatgcacaaa atgtaagtac
    30001 tcaaagtgca caaatagcta gaatggctgg aacaagttct gttcaaatag aaacattgga
    30061 aaaaatgtac aatactatca aatctggtat agatgaaact atgagaatag aagaaaacaa
    30121 tagagcttta attaaggata atacaaagag attggaagaa ttaaatacga ctataattta
    30181 taataagtag tattatatta agggctaatt tgtataaagt tggccctttc tatgtatatg
    30241 aggaggctag acattgaaat atttcgatta tatctgcaaa gatgatttag agagaatttt
    30301 tttgaaggag ccagaagatt ttagtgctaa gactgaaaaa gatgtgttga aatatgcact
    30361 gggcgctttt ttatacgttc cagctactca atataatatg atttataaga gtattatagg
    30421 ggatgttaag ggagttagac cattagctat ttgtttagaa gatgctgttg gagtaaatgg
    30481 agagttagag gctatagaga atcttagatt aattttaaaa aatattagca atgaaagtat
    30541 aactaataaa gatggaatac cactaatttt tgtaagaata aaagatgtgg agcaactgtt
    30601 gagaattaaa gagattataa ttaaaaatag tcattttata acaggaatat taattcctaa
    30661 agcaaattcg gagttaattg aaaattgtat agaagcttta gattctatga atcttcaaga
    30721 tatgtatgtt attccgatag tagagacaaa agaatttata tataatgaaa aaaaagaact
    30781 tagttttact aatttgtaca atgctatttt aagacataag tcaagaatat taagcattag
    30841 agtaggcata actgatatat tagggatgta tggtataagg agagataaaa acttctctat
    30901 atataataat ttgatttgtt cttcatttat attagatata atcacatatc ttcaaagacc
    30961 agagttagat atacctataa gtggtggagt atcagaattt tttgatatga cgaataagga
    31021 tataagaaat aagtatatag aagaaatatt attggataaa tatcatggat taattggaaa
    31081 gaccgtaatt catccaatgc aaattcaaat tgtacaagcg ctatctacag tatcatatga
    31141 agattttgct gatgctttgg atatactgga tagtacagac agtaagtatg gtgtgagcaa
    31201 gggtgtatta ggagagagaa tgaatgaaac aaatccacat tttttatggg ctaaaaaaac
    31261 attgattcta tctaaaatat atggtgtatt aaataaagga gtagactatg aagaattgct
    31321 taaattttaa ttgtgagatt gacattattg ataatccatt gaatttagaa ctaaatgatt
    31381 ttttagacgt aggaataaga aataatagca aaagacgttt tttgtttatc tcaaagaaac
    31441 tcggtaaaca tttagcatgt aagccaagtg agatggataa tttgggaaaa ttaatggcta
    31501 caatttataa tgaaaaaaat aaggaagaat caaatggtac tgttatagct tttgctgaaa
    31561 ctggtacagc tgttgggcat agtttttttg attacttatg tggagattat gaatttattc
    31621 atactactag agagcagttt gacgagttag aaaatctgga atttttagag gaacattccc
    31681 atgcaacaga ccagaattta tactttggta tgcttaacaa ctttaaattt ggagatgaga
    31741 taatattggt tgatgatgag attaccacag ccaatacatg catgaatata attaggaaaa
    31801 ttcaaagcat ttatcctaaa aagaaataca ctatatgctc aatactcaat tgggtaagtg
    31861 atgaaaattt aagtaaagtt actcgtttag aaagagagct agattgtaag atagattttg
    31921 tatacttatt taaaggaaac tttaaatttt atatagatga agatattatt tcaaattaca
    31981 acgataatta tagtattact aaattagaga ctatttcaag tgataaagta aatttagtag
    32041 taaatcatat ttatgttgat ttagaagatt atattggaaa taagaaatat gtaaaatata
    32101 caggaagatt tggaattaat agaagagaac aatttagatt aaaagaaatc gttaagagag
    32161 aaagtaaaaa aataactatg gccagtaaag aatctttaaa aaatgaaaaa gaaaagattc
    32221 tatttttagg tactgaagaa tttatgtata tacctatgct gtttgcaaaa gaaaaagaaa
    32281 ataaatatga tatatactat cattcaacaa caagaagtcc aattattaat attgataaga
    32341 ataattatcc tattaaaagt aaatttatgc taaagagttt ttataatata gatattcaaa
    32401 attatattta caatatagat aaacataatt tttctaagtg ttatttattt gtagagctga
    32461 ataaaagtga aaattcttat ctagaattta ttgatataat taaaaaaaca agtataaaag
    32521 aattaactat agtatgttgt agctaaagtg ttttaacagg ataaaatata tcacgatttt
    32581 aagataggag ttgagtcata tgagagattt tagcacgtat tctaaagatg atgttatttt
    32641 tttattaaaa gatatatcaa atttaattga agaagaagga aataaagaaa gagaattaaa
    32701 aatacaaaat ggtagacatt attcagaaat gataccaata gagtatgaag ttagtgaaga
    32761 ttatctgaat ttatatcatg agaaactaag agaaaataaa gataaacttg cattttctat
    32821 aggagttatg tgtgaaaaaa taattaaaaa aaatggaaat gatgtagtac tagtttcact
    32881 tgctagagca ggaacaccta ttggtatttt ggcaaaaaga tatataagaa gcaagtataa
    32941 tataagttta cctcactata caatctctat aataagagat aagggaatag atatgaatgc
    33001 aattgaatat atagtaaaga atcatccgag ctcaaaaatt caatttttag atggatggac
    33061 tggtaaggga actatatcaa aagaacttga aaaagcatgt gatgaactta atgttaaatt
    33121 taaaaaatat tttgactcaa cacttgctgt attagctgac cctgctggat attcaggact
    33181 ttatggaatt agagaagatt tcttaatacc aagtgcatgt ttaaattcaa ccgtttctgg
    33241 tttagtaagt agaactgtac taagagagga tttaattggt aaaaatgact ttcatggagc
    33301 taagttttat agacatttaa aagataaaga tgaatctatg aactatatca aaactataga
    33361 agaatgtttt aaaaatcaat ttaagaatat atctgatgaa gttgaaaact gggaaagtga
    33421 cattataaca agagatggat actttgatgt tttaaatata aaagaaaagt ataacataac
    33481 agatattaac tttatcaaac caggtgtagg ggaaacgaca agggttttac ttagaagagt
    33541 tccgtataag attcttgtta aagacttaaa tgataaatct ttagaccata tattcatatt
    33601 agctaaggag aaaaatgttg aagttgaaca aatggattta aaagcctata aatgttgtgg
    33661 aataataaaa aatatgaaag atatttagga aatatattag acaggagaat attgaatgat
    33721 tgttttttca gatttagaca ggagtatcat ttactccaat aaatttttaa atgctgatag
    33781 taaatatact aatatagaaa tttataggga aaaagaaata tcttatatat ctttagatac
    33841 tataaattta ataaagcaaa tacaatatta tggtatgttt atacctacaa ctactagaac
    33901 agtggagcaa tttaagcgta tagaatttaa taaatatggg atttattttc cttggagtat
    33961 aacttcaaat ggaggagtta tacttaaaga taatgaaata ttgaaatcat ggagtgaaaa
    34021 aatagataaa ctaaaatctg attatgagcc cattgaaagt atgattcata aatttaaaga
    34081 ctatttgaat gtagatggaa taactaattt taaagttgca gaagatacat ttttttatat
    34141 agttgttgat ttatcaagat ttaatttgga ttcaatcaaa gaatatacaa atatattgga
    34201 atcaaaaaat tggaagtttt atgtgagtgg cagaaaaata tattttatac caaaagaaat
    34261 ttcaaaagaa aatgctataa aatacttgac taaagaatta ggaatagaat atttttatgc
    34321 agttggagat tctattatgg actatggtat gcttaatatc tcaaataaat cctatgtatt
    34381 gaagcatggt gatataaata aaaatgaaat agaaaattca tttatatcaa gttctttcaa
    34441 tggaatgagt ggaactgaag agatactatc taacatttta gacgagaact gcctacttaa
    34501 catataatga ctgatatata aataataaac tagaaaaatc tgaaataaat tatagtaaag
    34561 tttatcatac taatttattt cagattttta tcaatttata tttaagtaag aactcaatat
    34621 gttaaaatga aggtggagtc attgcccaga ctgagataca tacctcatca tcataatttt
    34681 catacacatg tggtatagta ctgttaaaat aaaaactatc tccttcctcc aatagatatt
    34741 ccttatttcc aagtgttatc ttcattttac cttttattat atagccacat tcttctcctt
    34801 cgtgtgtcac aaattcactg tttttttgtg tatgaccatc tagcgtaaca agtataaatt
    34861 ctattttttt cccagataaa ttaggaacaa gaagttcata aaattttgta gaatcattag
    34921 ttactatttt ttttcgatta tttttcttta ctactatatc agagtcttct cttccaatct
    34981 cttcaaaaaa ataacctata tttatatcaa gagcttttga tattttccac attgctacaa
    35041 tagatggaac aaccatatta cgctcaattt gacttattaa gcctgtactt aaatctgaat
    35101 tttttgctaa agtagagata cttatatttt tagattttct aagagatcta attttgcttc
    35161 caacatctat attcattaga tacaaacccc ctcagacatg attataacat ataatttata
    35221 aagattaata aaataaaaaa aaagttttac aatacaagta ttttttatgc tatattgaat
    35281 tttctgaaaa gatgcattat aatagtatta atattcaata tattgaatat tgataacgat
    35341 attttaattt aatgaaatgg aggtaattaa gatgaagagt tttgtaagtc ctgataatat
    35401 ttgggtatta tgggcagttg taactggatg ggcagcattt agtatttatt tggaacaaaa
    35461 gtacaattgg gcatcaaaag tgtcgggagc aataatagct cttgtaggag caatgttatt
    35521 atcaaatcta aatattatac cagtagagtc tgttgtatat gaccaagtat ggggatatgt
    35581 tgttccttta gccatagctt tattgttata tcaatgtaac attaaaaaga tatggaaaga
    35641 aagtggaaga ttgctcatta tatttcttgt aggttctgta ggaactgtac ttggagctat
    35701 gattggattt ttggcattaa aaaatgtagt accagattta aatattgttg cagctatgat
    35761 gacaggttct tatataggtg gaaatgtaaa ctttgctgct atgtcaggcg cgtttgatgc
    35821 gccaggagaa ttagtatcag caacagttgt agcagataat cttttaatgg cattatattt
    35881 ctttgtctta atagctatac catcaattgg attttttaga aaacatttta aacatccaca
    35941 tgtagatgaa atggaaagta taggtataac tgaaggagaa acagtagcag caaattattg
    36001 gggaagaaaa gaaatatctt taaaagatat agcaatagcc attggtagtg cttttataat
    36061 tgtcgcagta tctgtagaac tatcaacttg gtttaaagct ataataccat tatcaaatcc
    36121 ttttttagca atgttaaata ctttatttgg aaatcaatat ctaataataa ctactttgac
    36181 tatgttgttt gccacattta tgcctaattt ctttggtgat ataaaaggag cacaggaatt
    36241 gggtacattt ttaatatata tattctttgt tgtagtaggt gttcctgctt caataacatt
    36301 aataattcaa aaatcaccat tactactttt atactgcgga ataatggtac taataaacat
    36361 gctagttaca tttatagttg caaaaatatt taagtttagt ttagaagaag ctattcttgc
    36421 atctaatgca aatattggtg gtcctacaac agcagcagct atggcaatat caaaaggatg
    36481 gtctaagcta gtagggccaa tacttatagt tggaacattt ggatacatag tcggtaatta
    36541 ttttggtctt ttagtaggaa atattttgat ataattttac gtgaaaaaaa gttgtttttt
    36601 agaaagattt ttatttatta gaaagatttt tatttaattg gaggttgtta tgattttcga
    36661 tggacattca gatacttggg cagatgtaac aataagaagg cttaacgggg aaaaagatgt
    36721 atttagaaga atccatcttg ataattttaa aagagcaaat atagaaggag ctatttttgt
    36781 aatatggata gacccaccat atgataaaga gcctgaaaaa agagttaaac aaattataag
    36841 ctctataaaa gatgaaattg aagatatgaa agatgttgta aacatagtaa aaaaatattc
    36901 agatatagat aaaggtataa gtgaaaacaa aataaatatt ttaattggtc ttgaaggatt
    36961 aagccatatt ggaagaaata tagatttact agattatttt tataaagaag ttggtgcaag
    37021 acatgcatca ttaacttgga atgaacagaa tgaacttgct acaggatgtt taggggatga
    37081 aaatcgtggt ttaactgaaa taggtaaaaa agcagtcaat aaaatggaaa aattgggcat
    37141 gatagtagat gtgtcacatt taaatgaaaa atcattttgg gatttaatgt ctgttgcaag
    37201 taagccagtt atagcttctc attctaatgc taggagttta tgtgatgcta aaagaaatct
    37261 tacagataat caattaaaag aaatttcaaa tacaggtgga ttagttggaa taaatagttt
    37321 tagaggattt actaatagta atcctaagaa gcaagattta atacatttgg ttgaccatat
    37381 agactatatg gtaaatctta taggtataga acatgtatct tttggttttg attttagcga
    37441 ttacttaggg aaagagacta taggttcttt tactggaaat gctagtgtgc caagtgtaga
    37501 tggtcttgaa agcacacata cagcacagaa catagtagta gagcttgaaa atagaggtta
    37561 taaaaagaaa gatatagagt taatctctag tggtaatttt tataggctct ttaaatatat
    37621 actaaaataa tata
//
LOCUS       DAENCV010000033        37247 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAENCV010000033 DAENCV010000000
VERSION     DAENCV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.033"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            90..632
                     /locus_tag="KQD82_001560"
     CDS             90..632
                     /locus_tag="KQD82_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4380035.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSYISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            646..3138
                     /locus_tag="KQD82_001561"
     CDS             646..3138
                     /locus_tag="KQD82_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4380036.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            3559..3957
                     /locus_tag="KQD82_001562"
     CDS             3559..3957
                     /locus_tag="KQD82_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380037.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(4174..4383)
                     /locus_tag="KQD82_001563"
     CDS             complement(4174..4383)
                     /locus_tag="KQD82_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380038.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(4415..4624)
                     /locus_tag="KQD82_001564"
     CDS             complement(4415..4624)
                     /locus_tag="KQD82_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380039.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            4923..5342
                     /locus_tag="KQD82_001565"
     CDS             4923..5342
                     /locus_tag="KQD82_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380040.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            5570..6025
                     /locus_tag="KQD82_001566"
     CDS             5570..6025
                     /locus_tag="KQD82_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380041.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(6181..6993)
                     /locus_tag="KQD82_001567"
     CDS             complement(6181..6993)
                     /locus_tag="KQD82_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG4380042.1"
                     /translation="MAEQKYYTLLTKAGKASIANATALGKKVDLVKLQLGDGGGSEYS
                     PTEEQTSLKEVVWEGNISNVKIDDDNSNWIVIETVIPGSIGGFMIREVGIFDSENKLI
                     AISKYPETYKPTADSGSVKDLIIRIILVVSNTSSVNLKVDPTVILATLKDIQELDKKI
                     DTTKITLTSGIETTKIELNTKIDNTKTDVTSKIGDTTLLNTTDKTSLVNAVNEVKEGL
                     DSIETTAEKTSYDNTVSKLAATNVQGAIDELCTSVNTARISLINDIKDMLPI"
     gene            complement(6995..7612)
                     /locus_tag="KQD82_001568"
     CDS             complement(6995..7612)
                     /locus_tag="KQD82_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG4380043.1"
                     /translation="MKLIDKLPSFYKNYMVEAIQDSYDSELSSLKENIDDTINQMFVN
                     TSTWGLDMWESILCIQNNEDLNYETRRSNIKAKMRSLGTTKLKVIKNICEAYTKTDVE
                     VTVLSNEFIFILEFIVNNCSYNSIVELDKVLENVKPCHLEHKFKMILLNKNELFCGTA
                     INTGETVTVYPWTPSNIEIFGEITISTGNDRSMEKVILYPKQEAI"
     gene            complement(7612..8661)
                     /locus_tag="KQD82_001569"
     CDS             complement(7612..8661)
                     /locus_tag="KQD82_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG4380044.1"
                     /translation="MYSNQTYETIKNRTLKNINLDVYKGEGSFLNDMVSGNTFELSKM
                     YVELSNIYKMAFIEDTYNQLLDKRVNEFGVYRKLGTEATGEVEFIGENGVEISNGTII
                     SYKDLSFVVIKDIVIGGEEGNISPVEALEIGTRYNLPSDCEFKVNDNIFGIEKITNIN
                     KFSGGTEIETDEELRERFFRVQKNQATSGNKAHYEEWALEVDGVYNAKVYPRWNGAGT
                     VKVLLFGQNNQAVEEEIITKCREHIEEEMPIGATLTVTTPIPLNITISASMELESGYT
                     LDMVKNSFIENINQYFRSITGEIVYTKIMGILISTSGVHDLSNLLVNNSTDNITIEDD
                     KIPSVTTLDFSEVEV"
     gene            complement(8654..9085)
                     /locus_tag="KQD82_001570"
     CDS             complement(8654..9085)
                     /locus_tag="KQD82_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG4380045.1"
                     /translation="MSNTIFPFIGVPEDYEIPKKEELGLFREVAWNFEKDEPIVENGD
                     FKIIEKNEAIKVWVYKCIKTNKNEHEIYSNDYGTELTDLIGQKYSKGLTESEASRYIK
                     EALLMNPYILQVDVVNTNFKKDILGAYIKISTIYGEVEINV"
     gene            complement(9085..9411)
                     /locus_tag="KQD82_001571"
     CDS             complement(9085..9411)
                     /locus_tag="KQD82_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG4380046.1"
                     /translation="MANSINEFIGIIRKEGKYYNKPSFFVGKIINKLPDLKVSMNNIV
                     LDKDNLLIDNWLFDRATKSFIITQSNNHTHELNESLTNQLDKGDTVIMFKVGEKFAII
                     SKLVSL"
     gene            complement(9425..10942)
                     /locus_tag="KQD82_001572"
     CDS             complement(9425..10942)
                     /locus_tag="KQD82_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG4380047.1"
                     /translation="MIINRSKDSSSVNINFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFADNKLAIGNIPKTNVKYTKMFISVNGYDTIMSAYTEASKTTKKKYMIEANLDKFN
                     IIEKGTVTLNIIFEEGFNLINTTFSESMENVKNKVLLVDQYGNKISEKVNNSLFKEVN
                     VIMQKVIQQQENSTTDIENEFKGIERTCSLKGYGDTSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGDYQIELELNFQNIMDEKSVGQDEQKEEISDLSGNGALNGREVKAEFT
                     AYYPSNDPMEGGLYQKMDGKKLVPSNNTCAAPEVLKFKTQIQVKGTGTNIDGKTYTVT
                     DRGGAIKVVNGIYKIDILMSSKKECYNFGRRKGTIIIGDGTGYTNSTGKVKELISIAK
                     GKKGCKYVWGASGPNTFDCSGFTQWCYKKIGINIPRVSRAQGSVGKQVEKKSLQPGDL
                     VFFSSKGPKGTIDHVGILIGNNEFIHSPHTGDVVKISSLKGSYYSKNYVGARRFL"
     gene            complement(11211..11633)
                     /locus_tag="KQD82_001573"
     CDS             complement(11211..11633)
                     /locus_tag="KQD82_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380048.1"
                     /translation="MEMWLRQAEDRFRFPVIPPSISINGKSTINSSNILKVGEIATFG
                     GVALKSINISSFFPNKEYSFCNYKGFPSPYDCVNKIQRWMNEGLILRFTVTETNINME
                     VIIEGFSYEERDGTRDVYFSLDLREYKRLQILRANPKK"
     gene            complement(11633..13963)
                     /locus_tag="KQD82_001574"
     CDS             complement(11633..13963)
                     /locus_tag="KQD82_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380049.1"
                     /translation="MGNVREEGINMYITDNYTPKMNQIISVTEALKRVTVSVSPSVNT
                     MANSIKSSMASASSSINSINSSIKKMTSSIKSLSNSIEKMNGSMNNSGSSNKDFSEGL
                     ETLGRSAENARSKLVRLSQVANSKKGRDTHKNVKAKNNESKNNKSDKPEKIKTVQDIV
                     KRVKTQTVDTSKKIVTGKLPELKNKVFNSNLVMGVKGNNIVQNTLNILNKIKNSKWIS
                     SISVKDLAKNVLIGTLSKLKQFAGRMWTGAISIKDKASSILGGIKDKISDITNGFSIG
                     GAVKKGVDLVGKGQNQKAVLTNVVQRTTGVKDKIEAKSEANNYYNDVIKDIALKSPFD
                     PSEVISMSTKAMTISGGKIDKSKDITQAMVNVRALNMETSSDQDVSAAFVSASKGNMD
                     ALNTLVGENYKTLDDALEGIKEKQNGLANEMAGTIPGLLSSASTYIDMGLNSMIEPFD
                     SILEGGLKKINSFLEKVPSGIGGFSQKVADVMNGKKIAENKQNPIETQVATALGHVPQ
                     SIGNTSQMLVQNIDFTALTNSLLPVVNLVNVLLDSINNKSPVAQGIISIFGTVVTTVF
                     QLIGPVVEAVSPIIMRIFTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIE
                     PILGAFFNILDKVCKIVKDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAG
                     TKAGDSGKAYSSKKGNNAFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNIN
                     IAKLADTIVIREEADIEKITSKLVASIQVAQLGGVL"
     gene            complement(13963..14130)
                     /locus_tag="KQD82_001575"
     CDS             complement(13963..14130)
                     /locus_tag="KQD82_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380050.1"
                     /translation="MYYYWKKKGIRPSLFYTMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGI"
     gene            complement(14157..14603)
                     /locus_tag="KQD82_001576"
     CDS             complement(14157..14603)
                     /locus_tag="KQD82_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG4380051.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(14717..15145)
                     /locus_tag="KQD82_001577"
     CDS             complement(14717..15145)
                     /locus_tag="KQD82_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG4380052.1"
                     /translation="MANMEARNVMSGTWGELWLDGDKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15159..16223)
                     /locus_tag="KQD82_001578"
     CDS             complement(15159..16223)
                     /locus_tag="KQD82_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG4380053.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIINNDIKKVIIDDYIGRVPNSYDNKCILI
                     VAIKEYLEELEKEELIEVGSLVDIDIEAQKQYLKSKNIDISNMKEQEIREANTGSKVF
                     LKAKIKMLDAMEDIDLSIEI"
     gene            complement(16228..16671)
                     /locus_tag="KQD82_001579"
     CDS             complement(16228..16671)
                     /locus_tag="KQD82_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380054.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(16920..17360)
                     /locus_tag="KQD82_001580"
     CDS             complement(16920..17360)
                     /locus_tag="KQD82_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380055.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17465..17662)
                     /locus_tag="KQD82_001581"
     CDS             complement(17465..17662)
                     /locus_tag="KQD82_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380056.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            17831..18151
                     /locus_tag="KQD82_001582"
     CDS             17831..18151
                     /locus_tag="KQD82_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG4380057.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18233..18739
                     /locus_tag="KQD82_001583"
     CDS             18233..18739
                     /locus_tag="KQD82_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380058.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19440..20195
                     /locus_tag="KQD82_001584"
     CDS             19440..20195
                     /locus_tag="KQD82_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG4380059.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20338..20766)
                     /locus_tag="KQD82_001585"
     CDS             complement(20338..20766)
                     /locus_tag="KQD82_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG4380060.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21187..21387)
                     /locus_tag="KQD82_001586"
     CDS             complement(21187..21387)
                     /locus_tag="KQD82_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG4380061.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(21570..22739)
                     /locus_tag="KQD82_001587"
     CDS             complement(21570..22739)
                     /locus_tag="KQD82_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG4380062.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            22975..23796
                     /locus_tag="KQD82_001588"
     CDS             22975..23796
                     /locus_tag="KQD82_001588"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG4380063.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIHIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(23872..25260)
                     /locus_tag="KQD82_001589"
     CDS             complement(23872..25260)
                     /locus_tag="KQD82_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG4380064.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25395..26153)
                     /locus_tag="KQD82_001590"
     CDS             complement(25395..26153)
                     /locus_tag="KQD82_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG4380065.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVGYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26155..26871)
                     /locus_tag="KQD82_001591"
     CDS             complement(26155..26871)
                     /locus_tag="KQD82_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG4380066.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(26873..27580)
                     /locus_tag="KQD82_001592"
     CDS             complement(26873..27580)
                     /locus_tag="KQD82_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG4380067.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(27696..28232)
                     /locus_tag="KQD82_001593"
     CDS             complement(27696..28232)
                     /locus_tag="KQD82_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4380068.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28334..29404)
                     /locus_tag="KQD82_001594"
     CDS             complement(28334..29404)
                     /locus_tag="KQD82_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG4380069.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHDEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29397..30479)
                     /locus_tag="KQD82_001595"
     CDS             complement(29397..30479)
                     /locus_tag="KQD82_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG4380070.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDLNKDINEDSYNNDYYNREEESIKETDEYN
                     DDSINFEAIYKDLEDIENKKGEDKHE"
     gene            complement(30466..30798)
                     /locus_tag="KQD82_001596"
     CDS             complement(30466..30798)
                     /locus_tag="KQD82_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG4380071.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            30997..32118
                     /locus_tag="KQD82_001597"
     CDS             30997..32118
                     /locus_tag="KQD82_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG4380072.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32246..34144)
                     /locus_tag="KQD82_001598"
     CDS             complement(32246..34144)
                     /locus_tag="KQD82_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG4380073.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            34459..35235
                     /locus_tag="KQD82_001599"
     CDS             34459..35235
                     /locus_tag="KQD82_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG4380074.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(35327..36079)
                     /gene="xth"
                     /locus_tag="KQD82_001600"
     CDS             complement(35327..36079)
                     /gene="xth"
                     /locus_tag="KQD82_001600"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG4380075.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(36240..37190)
                     /gene="galT"
                     /locus_tag="KQD82_001601"
     CDS             complement(36240..37190)
                     /gene="galT"
                     /locus_tag="KQD82_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG4380076.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT        12482 a         6045 c         4172 g        14548 t
ORIGIN      
        1 tatacattat agcttacata ttgattaata aattttaaag gagtataata aacagtgttc
       61 ataataattt taataaaaga ggtggtaata tgccaaagat tttagaaaat gtaaaagaag
      121 atatattaaa agtgtctcgg gatatgattt tagaagaaga ttactcgtat attagcataa
      181 ggaaaatagc tcaaaggtgt ggtataagtg caggtacagt gtataattac tttaattcaa
      241 aacaagaaat aatagaatat atcacaaaat ctgaatggga tttattgata aggcgaattg
      301 aatactccaa taaaaacact gaagattaca taaaaaaact aaacattatt tttactgaaa
      361 taagaaattt tataaacaaa gttcataata tccaatacaa tgacttttta aacacttttg
      421 aaacagagag attttttgaa atgaaaaaac acaaagacga ctttcatgaa cagctttcaa
      481 ataaagtata tgaagcttta gaaaaagaaa gtttttttaa taatgacaaa ttagtatgca
      541 atatcataat aaaaatgttt ttttcatatt caacatatct tcatatagaa tttgaagatt
      601 taaaacccta tattgaaaaa ttaatacatt aaggtggtgt ttaccttgaa tcataatact
      661 agtattaaga acaaggacag taatccaaaa atatttttag tacttacact ttatctatta
      721 ggtatattta tgggagctat tgatactgga atagtatcac cagcaagaac gattatacaa
      781 aatagccttg gagtaaatga aaaaacggga atttggatga ttaccatata cacattaaca
      841 tacgcttctg taattcctat ctctggaaaa ttagcagata aaataggaag aaaatatgtg
      901 tatctagtta gtatatttct atttggtagt ggttcattaa tatgtggatt atcatcacta
      961 ttttccaatt tctatatttt attaattggt cgtgttattc aagcaattgg tggtggtgga
     1021 atcatgccaa ttgctacagc agaatttgga actaccttcc cagaaaataa aagaggcatg
     1081 gctttaggtt tagttggggc aacatatggt attgccaata ttttaggttc aagcattggt
     1141 agcacaattc taagtatctt tggaactcaa aattggaagt ggctattttt tgtaaatctt
     1201 ccaatttgtt taatcatact tattggtgga gttttctgta ttaaaaacaa taagtctgaa
     1261 tctaaagaaa aaatagataa gcttggtaca ttgatgttga tatgtataat cgtatcacta
     1321 ttatatggtc ttatgaatat agactttttt aattttaaaa attctataca agatatatca
     1381 gtttatccat acttattgac atttattgta cttataccta ttttcatatt tattgaaaat
     1441 agagctaaag accctatttt aagctttgaa tattttttaa atccgagaat attgatagta
     1501 cttattttat ctttaattgt tggtattgga atgatgggta tggtattcgt tccacagtat
     1561 gctgaaaatg cattaaagat aaatgctggt tctgggggat attttgtagc tatattggga
     1621 ttatttgcag gtattgctgc tcctttaagc ggaagactta ttgacaaatt tggggcaaaa
     1681 aaaatactat tactaggatt ttctgtatct atgataggtt ctctttacct agttttaatt
     1741 gctctaaaaa ccaatactgt atttagtgta tgtgtaagtt taatatttat gggattagga
     1801 atgggattta ctatgggaac tcctctaaac tatatgatgt tatcaaatac aagattagaa
     1861 gaatctaatt cagcacttgc aacattatcc ttgatacgct caattggtac ttcaatttct
     1921 ccagctataa tgattggatt tatagcacat gcaggattat ctgtgcaaga taatattatg
     1981 gacttagttg gaaagccctc aactcctaaa atagttcaac ttgaagaact tgaaaacatg
     2041 atagatgact taaaatcaga ccctgaaatg gcaaaaaatt taaaaaatgt ctctatccca
     2101 aatatgaatg atacttcaaa tataaatatg gacatgacag gtggcaaact tccaaaatcc
     2161 ttacttaata aagttcagtc agcagataca accaacatta ccaatataac aaaagaaata
     2221 tcaacaacaa tgtttgatga aaaagttcca tctataatta ctaaaataca gaaaaatgta
     2281 caaaaaggaa tagatggaac acaaagtgga ataaacggta ttgaagatgg tgaaaataaa
     2341 ctaaatagtg gtattaaagg agttcaaact ggtattgaca atatggtaaa agctagagct
     2401 ggtattaatc aaggaataga tggtgtaaaa aaaggtattg ctggtgttga caaaggtata
     2461 aaaggtatgg aacaaggtct tgaaaaacaa gatgaggcca tcaaggaact tacaactgct
     2521 tataataaaa tacctactac agaaaaaact cctactgata acgaaaataa ctcaaatgaa
     2581 caaaaccaga gtaataccag taattctgaa tcagatgaca ctaagaatga ttctacagaa
     2641 aataagaata acaattccag cgagaataaa ccaaatgtaa atgaacaaaa agaaagttta
     2701 aatgctcaaa tacagcaact aaaaaagaca agagatgact taaatacaaa gttacaaaaa
     2761 aataaaactc aaaagaacga attaagtaaa aaactaagat caatggaatc tcaaaagaaa
     2821 gagctacaaa gcaagttaaa taattctata agccaaaaga aagacatgga aaaaagcctt
     2881 gatacaatgc aacagcaaaa aaaatcactc caagcagttt tagcaaagac acaagaggta
     2941 aaaactgaaa taccaaaggc ttttgataaa tcaaaattgg attatataaa ttccatagaa
     3001 aataatagaa caaaaataga aaatacacta caatctactc taaactcagg atttaaacag
     3061 atgtatataa cagtattctg tgtaaatcta ctagcattta taatattatt gttctataaa
     3121 gaaaataaaa ctagataaaa gagtgttata gtattatatt gtaaatttat cttattctta
     3181 aatattttat tctaaagaat aaattcataa aatttttgta attgattaat ataaattaca
     3241 atataatact atttttctct taatcttttt ctcttttaca gacaatcaaa aagcaatctt
     3301 agtttatcga aataaattta tacaatctat tcatacaccc ctttacattt ttataacaat
     3361 taaacctatt gttatatata caaatcaaaa taaaccatat attttcaact ataatatgtt
     3421 aaaatcatat tatgagcata ttaatttaag caaaatgtca tttagacttt aatttagtag
     3481 ttttatatta taaatttata tatttttaat tttaaatgtc atttagacat tactcataaa
     3541 aagaaaggag tgtattttgt ggatacttta ggaaaaagaa ttgcttattt gagaaactcc
     3601 aaaaaactta ctcagcgcaa acttatggat attttaaaat ttgaaaactt aggcaagtat
     3661 gaaacaggag atagaaaacc taattgtgat atactaatgt caatagctga ttattttaat
     3721 gttacgactg actggcttct ctatggtaaa gagaaggtaa atgtcaatag tagtgtaaaa
     3781 gaagataaag aagattactt acatgttact aatgatgaaa tgatgatatt aaatctatac
     3841 agacaactaa acgaacgtga taaaataaaa atagaaggaa tacttgaact taagatatct
     3901 gaatataaag acttaaaaaa gcattcctct aataataatg aagataaaat ggtttaaaca
     3961 tacataattt gatacgattt tacatcatct attaaccttc tttttttgta gtatattact
     4021 aaaatatgga tattggatta tttaaaaaca agtatagtct aatttcaagc acatttataa
     4081 tatttagatt atcttatcca tattttagtt taagtacttt gaaaaatata atacatctct
     4141 atgtataact tagattccta agaaatgcat tactcatgtt tttaattctt ccaacatctt
     4201 tctatacaca attgtacatc cttcataatt cccctcttta agaaggattg aacatgtact
     4261 agaatattta gttaaagtat ttaatctttc catatttaag ctttttatat tactgagtgc
     4321 tgtaataaaa tcatcaattg ctctacaaca ctctataacc ccatctgaat ccaatttaca
     4381 cacaagaata ttcacctact ttatatacta tttattaatc atcttctgaa tattctatat
     4441 taagtaattg ataaattgat gatttatatt ttttacctgt tcttcttcca tttaaaatag
     4501 ctgacaaata gttttcatta acacctattt tcttcgctaa ttccctttga gtcatattta
     4561 attcaataag tctttgcttg acttgcactc ccaaaaaagg tatccatttt tttcgagcat
     4621 ccatttatca acttcctttc taagtgattt atatttactt ttatattatt ttagcttaca
     4681 caccccctct atttagtata tatttttaat atgtacacat attaagtata taaatttatt
     4741 taatactaaa taagattttt tgattacttt gtgctaaaat taggatgtaa attcgtatca
     4801 ttaagttaat tataaagtca ttttgacctt aagtcaatgg tttttatgtc attttgacct
     4861 ttaatttgaa actatttaca tcttaaaaag tctttttgac attaattgaa tggagagtgt
     4921 ttatggatac tttaggagaa agaattgttt atttgagaaa agcaaaaaac cttaaacaat
     4981 atgaactaga agaaatgttg ggctgtgaca acttaagtaa atttgagaga aacattagaa
     5041 aaccaaatta tgaaatatta aaatctatag ctgagatatt taatgtttca gtagactggc
     5101 tattaaatgg agataatcta tcacataaat cagatttaat ttgcgattcc tcctcaaact
     5161 atccacttaa tagcataaac tctaatgaaa taaaactttt aaacaacttt agaaagttaa
     5221 atgattatga taaagccaaa atagaaggta tgattgaatt aaaacttcat gaatatgaaa
     5281 aagagaagga tttaggaaaa attgagtata acaaaaataa agatgagaaa atagataaat
     5341 aatttttatg ttgcttttga tacgatttta catcatttct ttgttctaat atttttattt
     5401 catttatttt cttttttata attttatgat aaaataatta gtatattttg ctttacttga
     5461 atgtaattat ttatacaaaa gtcaaacata atataaatgt agtaaatttt tagtttcaag
     5521 taatatctcg ttattataac taaatattta ttaaaattag gaggataaaa tgggcttaag
     5581 agacaaattc gcgcaatcat ttgctagatc aaaaactatg agtggccctg agaaaaaggc
     5641 taatgaaata atgggtaaat tgcttcttaa gaaagctata ttacctatcg ttttaatgtt
     5701 cgtgataata atcgctggag ctatgcttaa aatcaattct tgggttacac ttggcataaa
     5761 tttagttatt gctgttggtg ctttcttcta cataagaaat tctagtaaaa agtatcagaa
     5821 ctttaaacct tatgttggca atttgattag tttagagaaa aaaggtaaaa aagagtacgt
     5881 tgctattata aaacaaggta agttaccagt taagttacaa atagcttatg gtggtgaaga
     5941 ccttgaacat gttaagaaaa atcaaatggt tcaaataagt tataatccag atgctaaaat
     6001 agctattttg gttaatagac aataatttaa taaaaaaaaa gagttgtctt aaaattgatt
     6061 tttcacaatc aagtttaagc tcaactcctt ttttaatttt tatatgttat ttttattact
     6121 tttttattaa tatctctaag ttataaaatt ccagtctata caatattcca ttttttattt
     6181 ttatattggt aacatatctt ttatatcatt aatcaaacta attctagcag tatttacact
     6241 agtacataac tcatctatag ctccttgaac atttgtagca gcaagtttac ttactgtatt
     6301 atcataactt gttttctctg ctgttgtttc tatgctatca agaccttctt ttacctcatt
     6361 gactgcattt acaagacttg ttttatcagt tgtattgagt agagttgtat ctcctatttt
     6421 actagtgaca tctgtcttag tattatctat tttagtattt aactctattt tagtagtttc
     6481 tataccactt gttaatgtta ttttagttgt atctattttt ttatctaact cttgtatatc
     6541 ttttaaagtt gccaagatga cagttgggtc aacttttaaa tttacacttg aagtattaga
     6601 aactaccaat ataattctta taattaaatc ttttacactt ccagaatcag cagttggttt
     6661 ataagtttct ggatatttag atatagctat aagcttattt tctgaatcaa atataccaac
     6721 ttctcttatc ataaaaccgc caatactacc tggtattaca gtttctataa ctatccagtt
     6781 agagttgtca tcatctattt ttacattact tatattgcct tcccagacaa cttctttaag
     6841 agatgtttgt tcttctgtag gactatactc acttcctcca ccatctccaa gttgtagttt
     6901 aactaaatct acttttttac caagagcagt tgcatttgca atagatgctt tacctgcttt
     6961 agttaaaagt gtatagtatt tttgttctgc caatttatat cgcctcctgt ttaggatata
     7021 agattacttt ttccatactt ctatcatttc ctgtagaaat ggttatctct ccaaatatct
     7081 ctatatttga tggtgtccac ggatatacag taacagtttc tcctgtattt atagcagttc
     7141 cacaaaatag ttcattttta tttaacaaaa tcattttaaa tttatgttcc agatgacatg
     7201 gctttacatt ttctaatact ttatccaact ctacaataga attataacta caattattaa
     7261 ctataaactc taatataaat ataaattcat tgctgagtac tgttacttca acatcagtct
     7321 ttgtataagc ttcacaaatg ttttttataa ccttcaattt agttgttcct aaacttctca
     7381 ttttagcctt tatattgctt cttctagtct cataatttaa atcttcatta ttttgaatac
     7441 acaatatact ttcccacata tcaagacccc atgtggatgt attgacaaac atttgattta
     7501 tagtatcatc tatattttct tttaatgaag ataattcact atcataagaa tcttgtattg
     7561 cttcaaccat ataattttta taaaatgatg gtagtttatc aattaatttc attaaacctc
     7621 cacctcacta aaatctaaag ttgtaacact aggtatttta tcatcttcaa tagttatatt
     7681 gtctgttgag ttattgacaa gtaaattgct taaatcatgg actccagaag tacttattag
     7741 aatccccatg attttcgtgt atacaatttc tcctgttata cttctaaaat attgatttat
     7801 attttctata aaactatttt ttaccatatc taaggtatat ccactttcta gctccataga
     7861 tgcacttata gttatattta gtgggattgg agtagttact gttagtgtag ctcctattgg
     7921 catttcttcc tcaatatgtt ctctacactt agttattatt tcttcttcaa ccgcttgatt
     7981 attttgtcca aacaataaaa ccttaactgt ccctgctcca ttccatcttg gatatacttt
     8041 tgcattatat acaccatcaa cttctaaagc ccattcttca taatgtgctt tattcccact
     8101 tgttgcttga tttttttgta ctctaaaaaa cctttctctc agttcttcat cagtttcaat
     8161 ttctgtacca cctgaaaatt tatttatatt tgttatcttt tcaattccaa atatattatc
     8221 atttacctta aattcacaat cagatggtaa attatatcta gttccaattt ctagtgcttc
     8281 gactggactt atatttcctt cttcaccacc aatgacaata tcctttatta ccacaaatga
     8341 taaatcttta taggatataa ttgttccatt tgatatttca acaccatttt cacctataaa
     8401 ttctacttct ccagtagcct cagtacccaa ttttctatat acaccaaatt cattcactct
     8461 cttatctaat aattgattat aagtgtcttc aataaaagcc attttatata tgttagaaag
     8521 ttcaacatac atttttgaaa gttcaaaggt atttccagaa accatatcat ttagaaaaga
     8581 accttctcct ttatacacat ctaaatttat atttttaagg gttctatttt ttattgtttc
     8641 ataagtttga ttactataca tttatttcca cctccccata gatagtggat atttttatat
     8701 atgcacctaa tatatctttt ttaaaattag tatttactac atctacttgt aaaatatatg
     8761 gattcataag caaagcttct tttatatatc gagatgcttc actttctgta agacctttac
     8821 tatatttttg acctattaaa tcagtcaact ctgttccata atcatttgag taaatctcat
     8881 gttcattttt atttgttttt atacacttat atacccaaac ttttatagct tcattttttt
     8941 caataatctt aaaatctcca ttttctacta ttggttcatc tttttcaaag ttccatgcta
     9001 cttcacgaaa taaccctaat tcctcttttt taggaatttc atagtcctct ggaacaccta
     9061 taaaaggaaa tattgtgtta gacattataa actcaccaac ttacttatta ttgcaaattt
     9121 ttctcctact ttaaacataa taactgtgtc acctttatct agttgattag tgagagattc
     9181 atttaattca tgagtatggt tattagactg tgttattata aagctttttg ttgctctatc
     9241 aaacaaccaa ttatctataa gtaaattgtc tttatctaaa actatattat tcattgatac
     9301 ttttaaatct ggcaatttat ttattatttt tccaacaaaa aaggaaggct tattataata
     9361 tttcccttcc ttccttatta ttcctataaa ttcatttatt gaattagcca ctataccacc
     9421 tctcttataa aaatcttctt gctcctacat aattcttaga ataatagctt ccttttagac
     9481 tagatatttt aaccacatca ccagtatgtg gagaatgtat aaattcattg ttcccaatta
     9541 atataccaac atgatctata gttcctttag gtccttttga ggaaaagaaa actaaatctc
     9601 ctggttgcaa acttttcttt tctacttgct ttcctacact tccttgtgct ctggatactc
     9661 taggaatatt aattcctatt tttttataac accactgagt aaacccactg caatcaaaag
     9721 tatttggtcc acttgctccc cacacatatt tacatccttt tttaccctta gctatgctaa
     9781 ttaattcttt tacctttcct gtggaatttg tatatcctgt tccatctcca ataattatag
     9841 taccttttct tcttccaaaa ttataacatt cttttttact agacattaat atatctattt
     9901 tataaatacc attaactacc ttaatagctc ctcccctatc tgttactgta tatgtttttc
     9961 catctatatt tgtaccagtg ccttttactt gtatttgtgt tttaaactta agcacttcag
    10021 gagcagcaca agtattgtta gatggtacaa gctttttgcc atccatcttt tgatataagc
    10081 caccttccat tggatcattt gatggataat atgctgtaaa ttctgctttt acttctcttc
    10141 catttaatgc tccattacca cttaaatctg aaatttcttc tttttgttca tcttgcccta
    10201 cagatttttc atccattata ttttgaaaat tgagttctaa ttcaatctga taatctccat
    10261 tttgccatgt atgtttatct gtatctatat agaataatcc taccaatttt gtatacgaat
    10321 cttttacctt tacacctcta ccagttatac aacttgtatc tccatatcct tttagagaac
    10381 acgttctttc aattccttta aactcgtttt ctatgtctgt tgtactattt tcctgttgtt
    10441 gtatgacttt ctgcattatc acatttactt ctttaaataa agaattatta actttctcac
    10501 taattttatt tccatattgg tcaactaata atactttatt ctttacattt tccatacttt
    10561 cagaaaaagt cgtattgata agattaaatc cttcttcaaa gatgatattt aatgtaacag
    10621 ttcctttctc aataatatta aatttatcaa gattagcctc tatcatatac tttttctttg
    10681 tagttttgct tgcttctgtg taagcactca ttatagtatc atatccgttt acagatataa
    10741 acattttagt atattttaca ttagtctttg gtatgtttcc aattgcaagt ttgttatctg
    10801 caaatacttg ctttgcaata tcctctacta acttatcctt aaaattatag gacacttcac
    10861 tttgtaaaag taaaaatccc atatccttgg ctgtaaaatt aatattaaca ctgcttgaat
    10921 ctttagatct gttaattatc atccctctaa aaagttcttt ttcatctaca taaaagcaaa
    10981 ctgtgcttgc tattggaata tcaatttgtt gaaaattaat atcagaagct gactgtacta
    11041 tagaaaattc taatgttctt gaaggagact tataatctcc agaccaagaa actgaatcta
    11101 ctatattact tatatcatag atatttccgt tttttatgtg tacccaaatc tttataataa
    11161 aaaatcacct acctttttct ctgattttga gtaatttttt ataaattgta ctacttttta
    11221 gggtttgccc ttagtatttg aagtctctta tattctctta aatctagtga aaagtacaca
    11281 tctcttgtac catctctttc ttcataacta aaaccttcta ttatcacttc catatttata
    11341 tttgtttctg taactgtaaa tctcaatatt aaaccttcat tcatccatct ttgaatttta
    11401 tttacacaat cataagggga tggaaatcct ttataattac aaaaagaata ttctttattt
    11461 gggaaaaaac tagatatatt aatactttta agagctacgc ctccaaaagt tgctatttct
    11521 ccaactttaa gtatatttga tgaatttata gttgattttc catttatact tattgatggt
    11581 ggtataactg gaaatctaaa tctatcttca gcttgtctaa gccacatttc cattataaga
    11641 caccccctaa ctgtgccact tggatacttg caactaattt tgatgttatc ttttctatat
    11701 cagcttcttc tctaatcact attgtatcag ctaacttagc tatgtttata tttccaccat
    11761 tttttctgct tctatattga tttgcttctt gtttagttaa aaccatttca ccttcatgaa
    11821 gtctggtttg atagtcatta taaggaacat agttcaatcc aaatgcatta ttacctttct
    11881 tacttgaata agctttacca gaatcaccag cctttgttcc agcatatgga ttatctttgt
    11941 aatttttttt actcttttcc actagattta aaactgttcc tgtgatgctt ccatttttta
    12001 tcttattaat catagtttgc caccatttgc atatatcttt aactatttta catactttat
    12061 ctaatatgtt aaaaaaagct cccaatattg gctctataat tttccatcca gtttccaaca
    12121 gaggtcctaa ggtctcccat acagttttcc aaataacacc cagtgtctct ataaaattgt
    12181 ttatttgagg tgcgtattct cccaaaaagg taaaaattct cataataatt ggactaacag
    12241 cttctacaac aggtccaatc agttgaaaaa ctgttgttac tacagtccca aatatactta
    12301 ttataccttg tgctactggt gatttgttat ttatactatc taataataca ttaactagat
    12361 ttactacagg aagtaatgaa tttgttaatg cagtaaaatc aatattttga acaagcatct
    12421 gacttgtatt acctattgat tgtggtacat gtcctaatgc tgtagctacc tgagtttcaa
    12481 ttggattttg tttattttca gcaatttttt tcccattcat tacatctgca actttttgag
    12541 aaaaaccacc tattccgctt ggcacttttt ccaaaaatga atttattttt tttaatccac
    12601 cttctaatat gctgtcaaaa ggttcaatca tactgtttaa ccccatatca atatacgtgg
    12661 atgcactaga taataagcct ggaatggtac cagccatttc attagctaat ccattttgct
    12721 tctcttttat cccctcaagt gcatcatcaa gtgttttata attttctcca actaaagtat
    12781 taagagcatc catattacct tttgatgcac ttacaaaagc tgctgataca tcttgatcac
    12841 tactagtttc catatttaaa gctctgacat ttaccatagc ctgagttata tctttcgatt
    12901 tatcaatctt acctccacta atcgtcattg ccttagtact cattgaaata acttcactag
    12961 gatcaaaagg gctttttagt gctatatctt ttattacatc attataataa ttattagctt
    13021 cagatttagc ttcaatctta tccttaactc ctgtagttct ttgaacaaca ttagttaaaa
    13081 cagctttttg attttgacct tttcctacta aatctacacc tttttttaca gctcctccta
    13141 ttgaaaatcc attagtaata tcagatattt tatcttttat tcctcctagt atactactag
    13201 ctttatcttt tattgaaata gctccagtcc acattcttcc agcaaattgt tttaattttg
    13261 atagtgttcc tattaaaaca tttttagcta aatctttaac tgaaatagaa ctaatccatt
    13321 tactattttt aattttattt aatatattta aagtgttttg aacaatatta tttcctttaa
    13381 cacccattac taaattgcta ttaaaaactt tatttttaag ttcaggtaat ttaccagtaa
    13441 ctattttttt agaagtatct actgtctgag ttttaactcg cttaacaata tcttgtacag
    13501 tttttatttt ttcaggttta tcgcttttat tatttttaga ttcattattt tttgctttta
    13561 catttttatg tgtatctctt ccttttttac tatttgctac ttgtgataat ctaactaact
    13621 tacttctagc attttctgca cttcttccaa gagtttctaa gccttcacta aaatctttat
    13681 ttgatgaacc tgaattattc atacttccat tcattttttc tatagaatta cttaaacttt
    13741 ttatactact agtcattttt tttattgaag aatttatact atttatacta ctacttgcac
    13801 tagccataga actcttaata ctattagcca ttgtatttac tgaaggagaa acagatacag
    13861 taactctttt aagggcttcc gttactgata taatttgatt catctttgga gtgtaattat
    13921 ctgtaatata catatttata ccttcttctc ttacatttcc cactatatac cacctcctgt
    13981 caaaggacaa actccatatc catgcttcat tttttcaact tcttcttcaa tttctaaggc
    14041 gaaaaaagct tcaataagct ttaattcgcc tttatccatt gtataaaaaa gggacggtct
    14101 tatacctttt ttcttccaat aatagtacat tatcgtagtc ttggtatccg tccctattag
    14161 ttttttacct cttctattac tgcattttta ccataaccca tcacatcttg aaggattcta
    14221 tataagttag taatttcacc tggtaataat agttttcttg ccaattcttt tggtgttgga
    14281 gccttaaatt tatccattag ctccttattt ttaaataaaa gctttccatc caaatcacaa
    14341 acaccttcta taattgtaag catctgcatc tctggtaagt ctatatccgt tttcatattt
    14401 tcatcaattt taagacaagt attttgtatc tcttcatctt tacttattgt taaagctcta
    14461 cacattactt taaattcctg cccaaatata gttgataatc tcttaagttc aactatagta
    14521 cttggtcttt ctaagttttc tgtatctgca tttaaaagta aatctactat gtttatattt
    14581 tttgataatc cattttcatt cattattatt tgctccttta tcaatcaaat tcaatcaact
    14641 aactttttta cttattaact acctgaattt attaagtata tatctactaa atatcagtat
    14701 aaaaccaaat ataaaactaa attatgtcaa gaaaatcata ctcagtaaat gtgaaaggag
    14761 cttctatctc tccctttact ccaacctccc aatcagctaa agttaaatca tcaaatgcta
    14821 tattttttac tgctattctt tctgctccat aagaatctgg gtcatttaat ttagatatag
    14881 caacaaatct aggttcagaa cctctcttta tcttttctcc aattaacttg tgcattcttg
    14941 aactaacatg gtatagagtt attgaacctt ttcctttata tcccatatac tttgtatcag
    15001 tacccatttg acctgctatt ataatatcct cttttgtaaa ttccatcttt gcttgaaact
    15061 tctttacttc tgctacttta tctccatcaa gccaaagttc tccccaagta ccactcatta
    15121 catttctagc ttccatattt gccatattaa taatcctcct atatctctat tgataaatct
    15181 atatcttcca tagcatctaa catctttatt tttgctttta agaatacttt tgaacctgta
    15241 tttgcttctc ttatttcttg ttctttcata ttagatatat ctatattttt agatttcaaa
    15301 tactgttttt gggcttctat gtctatatct acaagtgaac caacttctat taattcttcc
    15361 ttttctaatt cttctaagta ttcctttatt gccactatta gtatgcattt attatcataa
    15421 ctattaggaa ctctacctat atagtcatct attattactt tcttaatgtc attatttata
    15481 atatctaaag tgtcaactat ttttattttc tggaacattt ctcctttttc tgctgttaat
    15541 tcagttaaag aatttactcc tctagcaatt cttatagctc ctgcttcttt aataagtata
    15601 agctctcctt tattaacctt tgattctgca tcaactttcg tcatcttagg tatatcgact
    15661 acatcactaa gtttagtata agttactgat tgacttaaag gtgtacctgc tataagtcca
    15721 gccaccctac ttgtaaactc atcaacactg tatttctttt ctccaactaa cacatcttct
    15781 gtagtaaaat taattatccc ttcatgattt cctacaactt ttcctaatac agctttaacc
    15841 ttaaccttat ctatatctct aagtttaatt atccaatttt ttatagcagt cttatcagct
    15901 tctactgctt ttggcataca tagataatta aattccttag tctctaaaaa atctaatgca
    15961 gtttgaatat ctgcttctcc ttctattaca taaactaata atttatttgg agtgttaaca
    16021 tttcccatca aggctaaatt tatatattct ttattttcag cagataaatc aactggtata
    16081 tcctcttttt catgtatttc attaagacct agtgccttag catctttaag caccattgca
    16141 attattcctc tagctgaacg ttctttaaca gttgtagcta gctccttaaa tgatatgttg
    16201 atacttggta atcctatagc catactatca ctctcctaaa tttaaattga tttcttccat
    16261 attttcatat actacatctt cttcatatac actatccaga tagtttagtg taagtgtaaa
    16321 gttcaataca taaattgact cttctttttt aatactttgt tctagctttg ttatatttaa
    16381 ttttctgtct gttaccttta tatttctgtt aaatagctta tttaattcat ctgaaacttc
    16441 atacattcta atactctttg actgtggttt tggtaagtat tttatagaga taacaatatc
    16501 tttgttattc gtatttaaca ttacaggtgt acaaataact gatagtatac ttacaaaaaa
    16561 acaagaccct tcagagcctt gcacactttc atcatcaata aaaatacttt cagtaaagtt
    16621 ttttttgaga atctcaataa ttgtttctaa tatttccttg tatttaagca aataatcacc
    16681 acactatcac taaatattct tgcttaatct gataatctta ctataaatga tataagtcat
    16741 ttttaaatat atatctcatt tattcctgtc aagtatcttc tatattttta ggtaatatta
    16801 ttcttttcaa tctatcacct ccttcaatat ggatttcttt cacaataaca ttatctcaca
    16861 tattttattg cctattgttg caatggtgta tataaagtgt tgtaaaattg tcctattttt
    16921 tataatatct cactcaattt aaatatcatc tttttcttta aattgtagca agatgttcta
    16981 tctaagtgta gtttcataga aatatatgtc atattgtttt ttgtcttact attataaaaa
    17041 agattaaaaa attccatttc tatatcattt agacacgtta gagcattttc tatcttctct
    17101 ttttctattt ctatatcttc tttttccatt tttaattttc ttaatctttc ctctcttttt
    17161 aacacctcat tttccacaga agagcttatg ttatatgtgt tactagtctt ttctgtatat
    17221 accattgctc cacatccaga gtattcattc tcacatcttt ttatgtccaa gttaatattc
    17281 tttatcttta tatccaaata cttataatta tgtaatcttt tatctgtttc tttaaataat
    17341 atatcttttt tgttttccat agtacttccc cttttttata tacttattat tagcctcaaa
    17401 tttacaataa tctctttagt gcacagtttt ttactaatct catagctttc aagaacatat
    17461 tgttctataa ctgtataaaa aaaacttctt ctatattttg ttctaaaata ctggaaatct
    17521 tcttagccat aagaagtcca ggatttttta tgcctctttc tatttgagat atataatcat
    17581 ttgtaattcc agcctttctg gcaagttgaa tttgtgtcat acctttgcat tttctataat
    17641 atttcaagtt atttttaaac attgttttat acctccatta ctattaagaa tattttgttc
    17701 ttcttcactt agattatata gaattatttg ttctttatca atagatttta gaaaaaaata
    17761 ttcttttata ttttattagt tttatttttt agaacaattt gttataatct atacataagg
    17821 tggtgttata atgtttgcca aaagattaag agaacttaga aaggaatttg gattgactca
    17881 aagagaactt ggagaaaaag taggcgtttc ccaaagagtc ttaggctact atgaaacaga
    17941 aaacagattt cctgatgaac acattttaaa taaattggct gatgtattta atgtatcagt
    18001 tgattacctt cttggaagaa ccttagttaa ggaaaatatt gatacagtag ctgcacatag
    18061 aaaaaatcca catgaagagt tacctgaaga agcacaagaa caacttaatg attatataga
    18121 atttttacta aataaatata aaaaaaaata aaccatatga tgagcagtct atttttctgc
    18181 tcttttaata taatttaaag gcaaacatac attctatttt agggggattt ttatgaataa
    18241 tttggataag ctttttgaat tagcttctca agaagagata ataattcatt ataccactta
    18301 tattgcaggt gatttagaag gactatatat aaacaaacat ggtattaaaa ttatatcatt
    18361 actcagtaat ttaaaacaaa actcaaaaaa attgacatcc atcttagctg aagaacttgg
    18421 acatcatttc actagccttg gatactatgt atcctcttat aacgattatt atacaaaaat
    18481 tattatagat aagtgcgaaa ataaagcctt aaaatgggct tgtgagttct taattactga
    18541 agaagatata ataaacataa ttaactctgg catcacttgc gtttatgaaa tggcagacat
    18601 acttaatgtg gatattactt tctttcaaaa aagattagaa tttttatcat taaaaaaaca
    18661 gtctttacaa cttggaaata ataaatattt aatattaact aatttgccgt atttctacat
    18721 atttgatcca atttcataat aaaaatagta gtaattttaa ctggcttaac agtaaaatct
    18781 atatatttat tttaacaaat tttggtttct ataaattcaa tattaatata taataatgat
    18841 atcatattat ttaaaattct aaaaacaaat atatgctttt gttaagaaag gttgtttgat
    18901 gaatgtataa agaaaagttg tttgataatt attttaagtt cttagcacta ttattttggc
    18961 caattatgtg gtataagtgg atagtaattt caaatggtac tcttgaaaat atgttattta
    19021 ccatatatgc aatcgtcgca attgttttca tcattttata ttcggtgttt atgataaaat
    19081 ataaagacat tacacaaata gatttctttt acaggataag tactctactt gcttttatat
    19141 ttacactttt tagcttttta atatacccaa agagtttatt tttcttgtat ttaaagatta
    19201 tttttactgg aatatacctt tattattcaa tagtaaaaac tcttaaattt aaagatgacg
    19261 aaggtgtagt gggtattatg agctcacttt tattaattgt aataactttg ttctactaaa
    19321 tttaattcta tttgacttaa tatggcttta aatattagac atctacatta ttttctgata
    19381 ttatttaaga tggtagtctt atattaataa tgaaatacac tatgaaggag ttaactatta
    19441 tggaaaaaaa agttttagca acagttggag aaaaagagat aactaatatt gatattgaaa
    19501 atgcattaaa atcattagac ccttatcaag ctatgcaatt taaaactgaa gaaggaaaaa
    19561 aacacttatt aaatgattta gtgaatcaag aattattctt cttagatgct aaagaagaaa
    19621 aattagatga agaagaaata tttaaattag aaatgaaaaa gatagaagaa aatgtattaa
    19681 agcaatttgc cataaataaa gttttatcta gtgttaatgt aacagaagat gaaaaagtaa
    19741 aattctttga agctaataag agctctttct ctaaaccaga aagtgcaact gctaaacata
    19801 tattagtaga tagtgatgaa aaagctaaag aaatacttgc tcaaataaaa tcagaagaaa
    19861 tatcttttga agatgcagct cttaaacatt cttcttgccc atctaaagat atgggtggtg
    19921 atttaggaac attcggtaga ggacaaatgg ttcctgaatt tgaagaagct gtgttctcta
    19981 tggctaaagg tgaagtaagt gaaccagtta aaactcaatt tggataccat ataataaaat
    20041 tagaagacct tcaagaaagt acagagtcta catttgatga agttaaggct gaagttgaaa
    20101 aaagtttatt atatcaaaaa caaaatgaag tttatggaaa taagataaat gctcttaatg
    20161 ctaagtacgg aaaccttgta aaatacaacg attaatcata aggtcacata taaaaaagct
    20221 gtcttttcac aatatttatt agaaaagaca gctttttata ttaaagttta aatattcagt
    20281 ttcatattat cattctttta ttgtaaagaa ctcagcttaa aagtttaaat aagattttta
    20341 attatcatta gctaaatttt tatatccacg tattatctca taacagtaac ctattacatc
    20401 tcttcttttt attatcccaa taaatgtgtc ttggtcatct ataacaggaa tgaaattttg
    20461 atttaaagat tttataacta aatcttcaat atctgcgttg atggaaacag gagaattatc
    20521 catttttcta tttatatctg tcactggaac atcttccaat ccctttaagt ccagatttaa
    20581 atcatttttt aatgtccata aaaaatcacc ttctgttata gttccaacat atttaccatc
    20641 cttacttatt attggaattg cagagtattt gtgatattcc attttttcta gggcttgcct
    20701 tatagtgtag tcttcatata tatatgctac ttcacttttt ggtgttagaa agaataatat
    20761 gttcattaca ctacccaccg ttactgaaaa cttataaata ttactcaata tttataatgt
    20821 tacttttttg cagtaacttt ctcctttctt attttctaag tattcttaat actatacgct
    20881 gtaacaagcg tttttccttt gctcatttta atcttattat gctttattaa tatttttata
    20941 tggtctatca gtatcactta ttctcttttt agaaaattta ctaaaaatta attgacttaa
    21001 acttagccat aataatttta ctctacacta ttaattttaa catatttatc tcattattaa
    21061 tgattttatt ttaataattt taatttattt tgctattaat tttatttaca aaaactgaaa
    21121 aaaccgctaa atgattagcg gcttatctct catattacta gaacactaat atatatgaat
    21181 ttttaattat aatctaacta cgttagcagc ttgaggacct ttagctccat ctactacttc
    21241 gaactcaacg ctttgtcctt cttctaatgt tttgtatcca tctccagtta tagctgagaa
    21301 atgtacgaaa acatctcctt caccttctac agatatgaat ccaaatcctt tttcgttgtt
    21361 aaaccattta actacaccag ttttcattaa taaatcctcc taaaaaaaca caaatattta
    21421 gctactcttt tctgtaacct tattactact ctatcatata tagctatgtt tgtcaattat
    21481 tattattttt atacattaat aatcaatttt tttactttat aattttaaat gattattatc
    21541 ttttacactt tatttttctt tataattttt taataattta tttttattat atggaatctt
    21601 atggacttat cactccaacc taaatcccat ggcaccaaca ttctatcagt attatgacaa
    21661 gttaccaaag gataccctct tgagtctaat cctgtcactg tcgaaacatg tgttattctg
    21721 cctttcttct cataaccaac aaaatcccct ggacgtaaat tataagcttc tttgtatact
    21781 tcttcatagg aacctttagc aattaatgaa cctcttccac tatacagtgc ataattttta
    21841 aaagcttgtg catttaccca tgcttttgta cctgctctct cgctataatt ccaaattgag
    21901 ttctttttga accttccact ttcaaacatt atctgtgatg caaagtttgc acaatctcca
    21961 ccttctggat tataatccct atagtttgga ttaaatttca taccatgttc ttcatcagct
    22021 gcggctccac agtatttatg tgcataatct atagcctttt tttgttcatc tgtaagctgt
    22081 aactctacac catcttgttg accaatataa tttcttatat catcagattt tatattttct
    22141 aaatttaaag agtctgcaaa agggtctgta taccactctt tagttatgac atattgatta
    22201 tctataattt ttacatttag ataatgatat gtacctattc taaaggcatt ttctttgtcc
    22261 ttttgatttt catatgaata cttatattca gtcgatactg aacagattat accatagagg
    22321 tctttttctt tttcttttat ttttttaatc ttaacccttg tctttatgtc tttaaatttt
    22381 acaccttgct tactagacca gttttctaaa tacttcatct tttttacttc attttcataa
    22441 gcccacagac caaatttttt atccgtctca taaagacctt ttaaaatgtt ttcatttttc
    22501 tctaatatcg ccttatttct gtagtcaaat aagttttcaa gcaatagttt atattggtct
    22561 tttacatcat caccttcaac atttgcagat tccatgactt tttcttccat atttttcata
    22621 cttaaatcat tgattaaaaa tacacctgct gtagtagcta aagacaaaaa tacaaatgcc
    22681 attatacctt tgatgtactt attcttattt ttcagtattt tcaaagctac acctcccaaa
    22741 tagtacacct tacaaatcaa attacaaaga atattctgtc acatatagta attttcatat
    22801 ttaataattc tctattttat tatgtccaat attaaaaatt aattgtatca atacactgtt
    22861 ttttttacaa ttttttattt tataatctac ttttttcttt tttttatagt aatatatatg
    22921 tatataaata ttttggtatc attataatta atacatacta atggagggtt ttttatgcaa
    22981 aaaaaagtcg cagcaattaa tgacttatct ggaataggaa aatgctcatt atcggttgct
    23041 atacctatac tttcagcatt aaaggttcaa tgttgtccat ttccaacggc catactttca
    23101 agtcaaactg gttatcctga atttactttc ttagacttta ctgatgaaat ggtaaaatat
    23161 tcaaatgttt ggaaaaattt aaaagtcaat tttgatagta tatatagtgg ttttttgggt
    23221 tcaaagcatc aaattgagat agtggctaat tttataaacg attatcccaa tgcttttatc
    23281 gtagttgacc ctgtgatggg tgataatgga gttatgtatc caatattcac agaagaaatg
    23341 agacaggaaa ttaaagaatt agttaaacat tcagatttaa ctactcctaa cttaacagag
    23401 gcttgtttct tgactggaaa tgattatact aaaagcgatt ataatagaga tgagcttata
    23461 catattgcaa aatctgtatc tgatttggga cctagcaaag tcgttattac tggtatatta
    23521 gaagatgata atatattaaa tttggcttac gatagagata atgaccatgt attctttaca
    23581 tcagtcaaat ataataattg ttcttacagt ggaactggag atatattcac atctatactt
    23641 tgtggtatgt tagtaaataa acatgatttg ggagtagcgg ttaatactgc aacagatttt
    23701 atatataaaa ctataaatta cacatctcaa tttgatactg atagaaatga tggagttatg
    23761 tttgaaaact ttttatcaga tttaactaat atataaaatc aaaaaaacta tccattttat
    23821 aaatacctat aaatcataaa atttaggtat ttacaaaatg gatattattt actaaactat
    23881 tggtatttca attgttacca tagcaccacc agttttcaca gaattttcaa tacataaagt
    23941 cccattatgt ttttgtacaa tattatttac aatatagagt cccattccat aatgagattt
    24001 agaatttcta ctcaaatctc ctgtataaaa ctgttctgta gcttttgaca aatctgcttt
    24061 agaaaaacca cttccacaat ctgtaactac aaattttaag tatttattac atactgatac
    24121 acttatgtat agtttactct ggcttggtga ataatctata gcatttgaaa ttatattcat
    24181 aatagctctg aataacagtt ctctatctat agttatattt ttaggcaaat tatcttgttt
    24241 ataaaactcc acatctaagt ttttagtact agctaatgct tctatttgca ctaagacatc
    24301 ttttataaac tcactcatac tgattgcttc tttatttaat atatagccat tctctgtttt
    24361 tgatatatct attaaagtat taatatattt ttctatttga gtgacaccct ttgagatata
    24421 ttccatgtat tctaataact caggattttc atttatttca attaataagt cagtatttcc
    24481 atgtataatt gttagtggtg tttttatatc atgtgccagt gcagatatct gttcctttct
    24541 attttcttct agcatccatt gtttttctaa agaatctttt aacgccaact tcattttatc
    24601 catagaaaaa aggacattat taatttcata aattttacta gattctacaa caaaatctaa
    24661 atcctgttgt tctatttttt ctgtagtatt ttttaaaagc tccatttcaa tattaaattt
    24721 cttgccaaat acttttgata atataacaat ttcaataaaa aatattatac taaaaataat
    24781 catacccaat gtctctggtt ttggtaaata tgctcttaaa attggagaac tatactctga
    24841 tactattttg tattttatta taaaaacttc atctttctta aaaaacttta tataactttc
    24901 tgaatatgca tgcctttctt caatatctct cattaaattc catacttctt ttgattcttt
    24961 tttattaaag gtacctgaaa ttaaattccc atctaaagta taaaccccat actcatatat
    25021 acttggaatt atatcttcag ttactttttc acttgaaata atctgttctt tatatttatt
    25081 gaattgtttt tctgcataat tagctggtaa aattacacct gatgataata atacagaaaa
    25141 tgataacatt aaaaggagta caattgaaat tgtcataata aagaataaag ataaatattt
    25201 tataaaaata gttcttaact tttcaacttt tttgttgtta gattttttac ctccaagcat
    25261 ttgtaccctg tcctccaata gtttaattaa ttcataatta atacctagcc agttttttat
    25321 ttgattattt tatatctttt tattgctaag ttatatttta acataataat ataaataaac
    25381 agtcaatttt tactctattc ttctgatttt ttaccttccc atttagaaaa ccaaatacat
    25441 aaaattacaa atgctaaaat tgttacaaaa ccacaaatta aacctgttga ttgatatgct
    25501 tttattgatt caacctttat atattcaacc gttttagaac ttgaaaaaga cgaccaaata
    25561 gttataaatc taacacccca tgcacaagga aaatactgcc aaataccaga acccaatcca
    25621 gttaataata atgctgaaag taacatttca actataccta ctccaattga tgctcctttt
    25681 ccgaacctta aacttaagaa taaatgtaat atgtattcaa ataaattaca cccaattaaa
    25741 attagaattg aaatcatata aaagcttgat gtaaagtaag ccttagatat accaaattta
    25801 aaacctaata cagacactaa tgttgctaca aaaccaagac atactaagat tatatattta
    25861 cttataagag taagatattt tggattagat gttgttaaca tatgtttata attccctgca
    25921 taatattctt gttcaacaaa cattgaacac acaacacctg acaataaagg aaaagctatt
    25981 gctataactt gtaaatatcc tactatcttt agtatattat cagctcctga aaccttataa
    26041 taccataaaa acaatcccat ccccattaaa gcaatgcaaa tgtgcattag tattataaaa
    26101 tttctcttta attttattaa atcagaacaa aatattctta ttaactgtct catattactt
    26161 cacctctcta ttattgaacc aaacccttgt agctaaagca aataatacaa ataaaacaat
    26221 agatattaat actcctggca aaataacagc attatttaaa aattcacttc cctctggtac
    26281 aggaagacca tttggcagaa tatgcaatac aacagccatt aatcttgaag gaatcgaata
    26341 tggaaacata taccacattt ctgtagttga gaaaacagca actccaacaa tagtgaagaa
    26401 catatttatt agaactgtaa caaatatacc aaattttgaa gctaaaaaca tacaaagagg
    26461 aatctgccac ataaatagta aaataattaa tatagttgca atgatatttt gacctattga
    26521 aatagtttct ttatagatta tcccaacaaa aactacacct attgcagaaa taattgatgc
    26581 tataaaacaa caaataatac agtaaatcat ttttccagtc caaacgtctt tcatttcaat
    26641 tggcaagggt aaaataccac gatatttact tttttcatct tttaatacta ttaaagaaga
    26701 tattaatgtt aatgctcctg gaagaaatgt cgtataccac cagttgaaag cagcattttg
    26761 ccctccacct agtccacaca aaagtataca aaacactgga actatccata tcaacttcat
    26821 tccaaaagtt cctttcatct tttgaaattc cactctaata aatctttcca tattactccc
    26881 ctctctttct attttcagct acaactttca taaaaagttt ttctaagttt tgttcttttg
    26941 atacttctcc ttgataaccc attactccat tagaaattat tcctatttca tctacaactt
    27001 gttcaacttc agacaagata tggcttgata aaataactgt aattccttgt tttggaaaag
    27061 aacgaatcaa ttctctaagt tcttcaatac caatagggtc aagaccattt gtaggctcat
    27121 ctaagattaa aagttttggg tgatttaata atgcaatagc aataccaagg cgttgcttca
    27181 tgcctagaga gaattgacct gaacgctttt taaaggtatc tcttaaatct acagtctcta
    27241 aaacttcttc aatgcgagag tcaggaagac caagtaaagt agtatgaact tttaaatttt
    27301 ctcgtgctgt taaattctca tatatagcag gagactcaat aagagaacca atatttgata
    27361 aatcctttct agtccatcta tgtccctcaa acattatttt tcctgaagtt ggatttaaaa
    27421 tcccagttat catttttaat aaagtagatt tcccagctcc atttggtcct aataatccat
    27481 atatagaatt agatttaatt gctagtgaaa tgttatcaac tgccatctgc tccttaaatt
    27541 ttttagaaac tccttttacc tgcaaaatat aattactcat aattttcatc ctttcttttg
    27601 tcaaacttat ttacaagaat cattctaaat cataaatata aggattttat aagaattaaa
    27661 aaacaaaaaa agatgtgtta aaaacacatc tttttttatc ttaattctat taattttcca
    27721 ttgtagtata tctctacttc ttcaatccaa atgctattat cttcttcttg gatgacattt
    27781 ttaataatat tatttatagt atatattata ttttgttcat tcaaataatc atctttccaa
    27841 gaaactctac tttttttatc tggttctatc aagtttattt taacaagatt atctttttcc
    27901 ttaacttcta cttccatgtc taaaccatta aaatactcat ttgataatgt atctaagatt
    27961 aatcgcagtt tatccttaag tgaactttct tcatcaatct gtaagtactt tactattttc
    28021 tcatcctttg aaattatctc tataggtatt ttagtagttt ttttagaatt aatcatttta
    28081 ctagttgttt cttcttttgc atcttcatcc ttctttacaa ctgctgattc tttagaatca
    28141 actagctcaa ctgcactgat gctataatcc atacatgccg tactcaataa agtagtagca
    28201 attattaaaa aacttactgc tattttattc ataagtatcc ctctcctaat tctctttcga
    28261 tacttattat accatagtat agattatatt tttatacttt taaatcaatt tataaattat
    28321 tatagtaaat tatttacaat tctaagctat ttgaatttat ttttatagta tattctttct
    28381 ctaaattcga cagattttta ttcaaaagcc cttttatttc cctcgtattt ctatacttgt
    28441 tgctatgttc ttcatcatgt tcatagtcat atgaatcatt gtcatattct tcatcattat
    28501 taaattcttc aattgcttct tttgacttta tatcacaatt aaatttatac ttatttactg
    28561 gtatatcaag tcttacctta gaatttggaa cttctatgtc caggaactta gcaattggca
    28621 tatcagagta caagtaaaat tcactttcat aatttgaatt tctacctatt ttctctttta
    28681 tatttacatt ttcaggtata tactcttcta tattattgat aaatacatct tcattctctg
    28741 aagatatata caatgactct gattctatgt ttacattttt aacgcctaaa attgattttg
    28801 ttgacaagtg aagattgtta ttagatatta agtttaggtt ttctaatcta tttactttgt
    28861 tctctgttat agctctacta aaacctccat aagaagtttt atatgttata gtatttgaag
    28921 ctacattatc gtatacaaat aagtgtgagc ttatacttga tgcatttatg tctacattat
    28981 ttggtacata tattataatt tctctataat catgtgaaaa catactattt atagatgtat
    29041 ttaataattg gtcgaaattt ttaatctttt taattttatt ttcatatttt ctttcacctt
    29101 ttacaactaa agtcttatct tttaaagtta cattgtattt gtaaaactca ttatttcctc
    29161 ttgttgttac acgcacatct tcacttgaac ttttctttat tgtaacgttt atattgtcaa
    29221 cagctatatc taacttgtca atatttactt tttcttgata aagtttattt tcttttttaa
    29281 attcagcttc cttttctaaa ccaaagttta ttaggcttgg cataactaaa atccctgacc
    29341 atgttgtccc tattatacct attaccaata aaactgcagc aaatatagca agttttttat
    29401 tcatgtttat cttctccttt cttattttct atatcttcaa ggtccttata tatcgcctca
    29461 aaatttatac tatcatcatt atattcatca gtttctttta tgctttcttc ttctctattg
    29521 tagtaatcat tattatatga atcttcatta atgtctttat tcaaatcttc attaatgtct
    29581 ttattcaaat cttcatcatt taaagtagct ttttttcttt tttccatctt ttcatttgcc
    29641 tttatataaa tattttttct ctttatccaa tgtatataat tatttagtaa ttgtttacct
    29701 agttttatgg caaataataa aatttgccat gcaagtatct caagccctac aaatgctatg
    29761 gttgcgaaaa tacaaaacat cataacatct ggaaccattg ctataaattt aatcacaaat
    29821 ggaattaata taacaaaagc aattaatgag catactaatg caatagtagc agatacaata
    29881 gcacatacaa acgcaaatgc tggaacaatt agaaataaag ataacactac taataacccc
    29941 tttataactt gtctggataa tcctcctacg ccttcttcta tacttggtgt aagtttatca
    30001 tttatatttt cttttgattt attgtacttt tccttagctt catctttcaa gatagagtaa
    30061 tcctttttta catttgattt aaatctacta aatttaccat ttaatatgtc tgtattcatt
    30121 atttttccat ctatattttc ttttgattta ctatactttt cttttgcttc atcttttaag
    30181 atagagtaac cttttttcat atctgattta aatttactaa acttatcatt caaaacatct
    30241 gtatttatta ttttttcctc ttcaatattt ttttctttag tttgtaagat aagttctttt
    30301 gctatagatt ttggtgagcc aagtgatgct attatctcca tatcactctt tccctctata
    30361 agaccatcta cgaaatactc ttcataatct ctcaatatat cattaacttc gctctcagaa
    30421 aaatcttttt ttagataatc ctttaaaatt tcaagaaact cagttttatt caagttcatt
    30481 acctccctca tcttcactta ttaaaatctc aacagccttt acaaattctc tccactcttc
    30541 catcagattg gataaattct cttttcctag tgaagttatt ctatagtatt ttctagcagg
    30601 accttcatta gattctagta tatatgtttc gaaatacaaa tcttttgtaa gtcttcttag
    30661 aataggatat attgttcctt catttacatc tattacttta gagatgttat gtactatctc
    30721 atatccatac atatctttct tagatatgag agcaagtaca caaatttcta aaacaccctt
    30781 tctaaattgt gtgttcatat actccatcct ttctttaaat acacagtatt gtgttttaca
    30841 aggtactatg ttaaaacttc ttatatcctc ataataacac agtactatgt tctgcacaat
    30901 agttattttt aaatattctt gttgattaaa tccttctata aatgtataat actctaatca
    30961 gatatatttt atgaaagtgg tgataaattg gctaaaatga aagatgataa aacaatatta
    31021 atcctaactg ctcaatttgg tgctggacat ataagtgctg caaaagcagt caaagaatgt
    31081 attattgaaa aatatagtaa ctataatgtt gttatacaaa actttataaa tgcaagtatt
    31141 cccatgatga ataagcctat ggtaaaactt tatgaaaaca atacaaaata tactcctgga
    31201 ttatataatt actattatta ctttaagaaa tcgtttgatt caagacatga tttatctcat
    31261 aaattatata cacctaaact ttctgagtat attgctgata taaatccaga tttgattatc
    31321 tctacatttc cactagctgc tgcttgtgta aataacttta agataaaaaa tcctgatata
    31381 aatataccta cacttacagt tataacagat gttgtagata gtatggaatg ggtttttgaa
    31441 aatacagatt tgtattttgt tccttctcct gaaataaaaa atagattttt tcagaaagga
    31501 ataaatcctg actccataaa ggttactgga gttcctgttg ataagagatt tcaaattgaa
    31561 agtaaagaac tatgttgtga taaatataga ctattacttc taggtggagg aagaggtcta
    31621 tttgatatag atgaggattt tatgcattgg atagatgaat ttattgaaga acatagtaat
    31681 tctatagaaa ttacaatagt tactggtaaa aacaaaaaat tatatgacaa tctaactcat
    31741 aaaaaacctc taaaaaatat taaagtactt ggatttgtaa acgacatgta taatctaata
    31801 agagaatgtg acctaatgct aactaagcct ggaggagcaa ctatatttga agctattcaa
    31861 tcacaaactc cagtactcgt aaaaatgcca aaagttggac aagaaattga aaacgctaaa
    31921 tttataatag ataagggtct tggcatgatt tatagtgatg acctagacct taaaaacata
    31981 ttttataaat tagtatcaaa tgaatttgat tctataataa actttatgaa aaaaaattta
    32041 aaagaattta aaacagtcat tcatccagag aaaattgctg attatatatc agaattaata
    32101 gataatcatt acagttaaat tgtactatta aaaaatttca aacataaaat taaaggcaat
    32161 caagtatgct tttacacttg agaatttact catagtgttt aataatactt gattgccttt
    32221 atcatatcaa aatcaaattg ttatattagt atatattttt cttgtactgc atatacagtt
    32281 gttgtatttc ttccatactt ctctttaatt ctatttgtaa atctgaagca gtctggtaat
    32341 ccttattttc tatagcttct ctcactctag tgaatagtga ttctctagtt aaagtatcct
    32401 tcataatatt gaactttaaa tccgatattg agttccaaac tacctcatca agcgcatcca
    32461 tattttcttt tgtgtgtatc ttgacaatac ttcttatttt ttgaatccca gcctctatta
    32521 ttctagtttt taatttcttt tgccacttat ctatagctcc aatcttaaat gattctatta
    32581 tactatcagt aaatacatca ccttgtttta aagattttaa ttttgaagca tatatttcta
    32641 agtttttcat attttcataa actgtagctg gtggtattcc aaatcttgca tttctttctt
    32701 ccaaactata atgttcaaat acatcatttt catctctata tgctctatct ttttctaaat
    32761 agaaactttc ttcacctaca tttttagata actctttttc aagttctttt gtactaagac
    32821 cactcttagc tgctgctttt ataccatcca acattgtttg ataacatcct gctattacta
    32881 aatacgtgtt agatagaggg cttggtgacc ttaattcaaa tcttacagtc tttggatttt
    32941 tcatatctct tattaatcca acaagcactg acctatttct tgatggaatc tcataactat
    33001 gccctaaaga tgtaactatg catacaggag cttcaaatcc aggaactaat ctattaaatc
    33061 catcattaga agctgttaca attgggttta atacttcata attgtaaaga agtcccataa
    33121 gagccccata tcctagttca cttagataat cttcttttaa atcttttgga gcaaatagat
    33181 tttttatact tccatccttt aacttcgcac ttactcctac atgtgtatgt ccaccacttc
    33241 ctgctactcc atgaattggt tttgctttaa atgtaacttc tagtccatgt gatgtaaata
    33301 catcctctat aacatctctt actaataatt cattgtctgc tgcttgtaat ggtgtagaaa
    33361 acttccaaga aacctctaat tgctccatag catggtttgt tcttccatct atactaatag
    33421 aactttgtat tcctccgacc tctttgtgag ccatttcagg gtttacacct agcttttgaa
    33481 gtataattaa acttttctct aaacaagttc ttataatacc atgagttctt ttccagtatt
    33541 gctcttttag gctttgtgat acatataatt tctctaagtc agctttatcc tcaggagtat
    33601 taacccagaa ctcaagttct gtagctgctg ttaacattat ttcctctata tcatctactg
    33661 aatctattcc tatgttattt attacatgtg gatattctct aaatatttca tacattgact
    33721 ttttaaagta cttatctgct ttttgaagta ctcctctaga acatactttt ttattatcat
    33781 gtattaaaaa tgcaggtatc tttaatgttc caacaggtag acctacttct tcgtctatat
    33841 attccatatt gtaatctaca taccaatgac aacttttatc tggcattaaa tctactttcg
    33901 cattatttaa tgtagctata ttataaagct caacacttga accatcagtc tgtatagctg
    33961 attccaaaaa tttatctata tcctccaaaa ataattctac aggaatcttt tcatctgttg
    34021 catttcctcc taaatcaacc cccattaagg aaacaaatct tatattttta ttgcttttta
    34081 ggatttctct taattcttca ttagtgtgtt tatctttctc tattacgtat aatagactat
    34141 ccatttttcc actctcccat aatttttatg tatattttat tttgttaaat acaagataaa
    34201 tttcattttc cttacattta tgaatttttc ttgcaaatta ttcgtgtttt attcgtgttt
    34261 ttatttatat tattatatat caagtataaa aagttcgcaa tattttttat tggaaaaaat
    34321 attatacata caatagttta acatttttac aaatatgggg tatactaaaa taaaagtatt
    34381 tttatttgaa aatttaattt tatttataat ataatctata gtaaaagaaa taataaaaaa
    34441 atttaggaga taaactttat ggaatacata attttcgatt tggaatttaa ccaaggattt
    34501 gataaaaaac tcaataaaac tgtatccaat gaaaagtgtc cttttgaaat tatacaaata
    34561 ggagcaatta aattggattc taaattcaat ataatagata cgtttaatag ctatgtaaaa
    34621 cctacaattt ataaagacat acatcccttt attagtcgta tgactaatat taaaaattct
    34681 gattttaatg attcgcctac tttccctgag gtctacaaca attttataaa atttatatca
    34741 tcacaagacc ctatattatg tgtttgggga gctggagatt taaaggaatt atacaggaat
    34801 atcaactatc acaagctacc atcaaattcc ttacctaaat cttatattaa tatacaacaa
    34861 catgcatcta aatatttcaa taatccagca ggaaaaagca ttggcctaca aaatgctatt
    34921 tctatattag aacttgatga aaaaatgtct tatcacaatg ctttaaatga tgcatattat
    34981 actgctaaag tttttattaa gatatacaat ccaagtatag tcccagatat atatctttat
    35041 acgagtataa aacctaaaac tataagatat tctaataaaa aaagagttga ttatgataag
    35101 ttatttgatg aatttaggaa aattttaaat cgtgaattaa ctaaagacga gaaaaaaatc
    35161 attaacttag cttataatat ggggaaaaca aatcaattta cattagaaaa tgttaagcaa
    35221 agaaaaaaca aatagagttt atttagataa aaaataggca tgtagattaa aaaactagtt
    35281 aactttaaat ttatatctac agcccaattt ttatcataat taatttttat tttatctcta
    35341 attcaactgg acaatggtct gaacctaata tttcagtgtg tatatccgca cttactaatc
    35401 tatcttctaa ttttttggat gcacaaaaat aatctattct ccaccctgca ttattttttc
    35461 ttgcattaaa tctatatgac caccaactat aaacaccttc tttatcagga ttaaaatatc
    35521 tatatgtgtc tataaaccca ctgtctaata atttagaaaa cttctctctt tcttctttag
    35581 taaaacctgc atttttcata ttatttttag gattttttaa atctatttct ttatgtgcaa
    35641 cgttcatatc tccacacaca attacaggct tatgattatc tagtttggtt aaataatcta
    35701 taaagtcgtc ttcccatcta gttctatatt ctagtctctt taattcagat tgagaatttg
    35761 gcgtatatac tgtaacaaag taaaaatctt caaactctag agttatcact ctaccctctt
    35821 tatcatgttc ttctatattt attccataca ttacttttaa aggctctttt tttgtaaata
    35881 tagcagtacc tgaataaccc tttctctcag cataattcca atattgaaaa tatcctggta
    35941 aatctaactc tatttgccct tcttgcaatt tagtttcttg taaacaaaat atatctgcat
    36001 ctacttcttt gaaaaagtct aaaaatcctt tcccaacaca agctcttatt ccattaacat
    36061 tccatgatat aaatttcata cttatactcc tcatctaaac attgacacac tccccatagc
    36121 taaagatggg ggcttttcaa caattttatt gtaaattctt aattcacttt tattttaaca
    36181 atgctatact ataatttcat ctaaaattac tatttactta atacctaaaa atatatgttt
    36241 taatcaattt ttaacttttt tgttaaatct tcagggctca ttgaagaaac atacatattt
    36301 gttccaagtt caaatcctcc aaaattatat tttcttggga ttatgtgcat atgaacatta
    36361 aaatacgatt ttgtttcaat ctttgttgga tgagtgtgaa tacataaatt aaatggcata
    36421 tatccattga tattgtataa cttcttaaac aacttttcaa atattgtaga aagctcctgt
    36481 atattattat catttaactc accaaactct attttatctt taaatattat tcttacttct
    36541 ccactatact tggttgcata tggtataagt accaaaaaag cttctccatt ataaacaact
    36601 cttctatcta aacttatttc attttctatt aaatcatcat acaaagagct tttattgctg
    36661 tcataatact cctttgaaac caacagttca ttcgtaattt caggtggaat aaaagacata
    36721 gacaatattt gtgcatgtgg atgcatcaaa gatgctcctg cctttctcaa gaagtttttg
    36781 aagatactaa catactctac tttatcatct ttcgacaaat ctctaaatct atttctatac
    36841 ataaaaaaaa catcttcaaa ttctttttga ctcatgttat aaaaactacg attatgttta
    36901 tcgctctcta tcattacttc atgaacacca tatacatctc ttgccatatc atcaattata
    36961 ggaaatttat tgtagacaga ttttacaagc catccttctt cactatctat ttcaaataat
    37021 gcatcagttg catgagcttc atttcctttg caaaatgggc attcttcatc atattcatta
    37081 gtttcttcat cttcatttgg gatatctgct gtatctaaag gtctttttaa cctatctgta
    37141 gcaaatataa caacatcatt ggtaattgga tctattctta attctttcat aattaacacc
    37201 tcttagttaa tagcctttaa tataattata tgatatttat cgtatct
//
LOCUS       DAENCV010000034        35999 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAENCV010000034 DAENCV010000000
VERSION     DAENCV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.034"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            382..1203
                     /gene="yidA"
                     /locus_tag="KQD82_001602"
     CDS             382..1203
                     /gene="yidA"
                     /locus_tag="KQD82_001602"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG4380077.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCMNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1340..1489)
                     /locus_tag="KQD82_001603"
     CDS             complement(1340..1489)
                     /locus_tag="KQD82_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380078.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1496..1981)
                     /locus_tag="KQD82_001604"
     CDS             complement(1496..1981)
                     /locus_tag="KQD82_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380079.1"
                     /translation="MSQIEKYNSLNNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1994..2746)
                     /locus_tag="KQD82_001605"
     CDS             complement(1994..2746)
                     /locus_tag="KQD82_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380080.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2923..3552)
                     /locus_tag="KQD82_001606"
     CDS             complement(2923..3552)
                     /locus_tag="KQD82_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380081.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3513..4076)
                     /locus_tag="KQD82_001607"
     CDS             complement(3513..4076)
                     /locus_tag="KQD82_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380082.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4086..4310)
                     /locus_tag="KQD82_001608"
     CDS             complement(4086..4310)
                     /locus_tag="KQD82_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380083.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4315..5406)
                     /locus_tag="KQD82_001609"
     CDS             complement(4315..5406)
                     /locus_tag="KQD82_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4380084.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5435..5893)
                     /locus_tag="KQD82_001610"
     CDS             complement(5435..5893)
                     /locus_tag="KQD82_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380085.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5886..6971)
                     /locus_tag="KQD82_001611"
     CDS             complement(5886..6971)
                     /locus_tag="KQD82_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG4380086.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6998..7183)
                     /locus_tag="KQD82_001612"
     CDS             complement(6998..7183)
                     /locus_tag="KQD82_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380087.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7345..7677)
                     /locus_tag="KQD82_001613"
     CDS             complement(7345..7677)
                     /locus_tag="KQD82_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG4380088.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7933..8769)
                     /gene="nadC"
                     /locus_tag="KQD82_001614"
     CDS             complement(7933..8769)
                     /gene="nadC"
                     /locus_tag="KQD82_001614"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG4380089.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8750..10051)
                     /locus_tag="KQD82_001615"
     CDS             complement(8750..10051)
                     /locus_tag="KQD82_001615"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG4380090.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10064..10978)
                     /gene="nadA"
                     /locus_tag="KQD82_001616"
     CDS             complement(10064..10978)
                     /gene="nadA"
                     /locus_tag="KQD82_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG4380091.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11396..12853
                     /locus_tag="KQD82_001617"
     CDS             11396..12853
                     /locus_tag="KQD82_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG4380092.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13076..13504
                     /locus_tag="KQD82_001618"
     CDS             13076..13504
                     /locus_tag="KQD82_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380093.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13564..13872
                     /locus_tag="KQD82_001619"
     CDS             13564..13872
                     /locus_tag="KQD82_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG4380094.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(14019..15041)
                     /locus_tag="KQD82_001620"
     CDS             complement(14019..15041)
                     /locus_tag="KQD82_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG4380095.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15216..15722)
                     /locus_tag="KQD82_001621"
     CDS             complement(15216..15722)
                     /locus_tag="KQD82_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG4380096.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15782..16483)
                     /locus_tag="KQD82_001622"
     CDS             complement(15782..16483)
                     /locus_tag="KQD82_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380097.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16701..17771)
                     /gene="buk"
                     /locus_tag="KQD82_001623"
     CDS             complement(16701..17771)
                     /gene="buk"
                     /locus_tag="KQD82_001623"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG4380098.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17824..18399)
                     /locus_tag="KQD82_001624"
     CDS             complement(17824..18399)
                     /locus_tag="KQD82_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG4380099.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18401..20188)
                     /gene="iorA"
                     /locus_tag="KQD82_001625"
     CDS             complement(18401..20188)
                     /gene="iorA"
                     /locus_tag="KQD82_001625"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG4380100.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20249..21508)
                     /locus_tag="KQD82_001626"
     CDS             complement(20249..21508)
                     /locus_tag="KQD82_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4380101.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKVLDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21770..23770)
                     /locus_tag="KQD82_001627"
     CDS             complement(21770..23770)
                     /locus_tag="KQD82_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380102.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23778..25664)
                     /locus_tag="KQD82_001628"
     CDS             complement(23778..25664)
                     /locus_tag="KQD82_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4380103.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYPFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCHSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            complement(25691..27532)
                     /locus_tag="KQD82_001629"
     CDS             complement(25691..27532)
                     /locus_tag="KQD82_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4380104.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(27663..28082)
                     /locus_tag="KQD82_001630"
     CDS             complement(27663..28082)
                     /locus_tag="KQD82_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG4380105.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            28395..28577
                     /locus_tag="KQD82_001631"
     CDS             28395..28577
                     /locus_tag="KQD82_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380106.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            28906..29928
                     /locus_tag="KQD82_001632"
     CDS             28906..29928
                     /locus_tag="KQD82_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG4380107.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKIRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(30013..30615)
                     /locus_tag="KQD82_001633"
     CDS             complement(30013..30615)
                     /locus_tag="KQD82_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG4380108.1"
                     /translation="MKSLKKILIVTGCLTLISTSLVFASTNNDVNVSKSNEEQTTNSF
                     TITGYEFIYENAPQPIKSDYEKKCKELNINPSKDDTIFINEKFIDSYNINIDDYNIEP
                     KAFIIRYKKDLNRFDVTGPISYSVNINTTVVGYGHVEKGNPVHLVQLLCQQINGSDID
                     PDSKFGPATYNQVKILQGRLGVTKDGVVGKATWQAAGERY"
     gene            complement(30666..31700)
                     /locus_tag="KQD82_001634"
     CDS             complement(30666..31700)
                     /locus_tag="KQD82_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG4380109.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKIYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKDPKENTVGNNITQIDISIDNDFMLYLVYLWLFVTI
                     AIAVYTFIEYTKFKNLVVDVSYDIEDNDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            complement(31693..32070)
                     /locus_tag="KQD82_001635"
     CDS             complement(31693..32070)
                     /locus_tag="KQD82_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380110.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            32573..33485
                     /locus_tag="KQD82_001636"
                     /pseudo
     CDS             32573..33485
                     /locus_tag="KQD82_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(33681..34268)
                     /locus_tag="KQD82_001637"
     CDS             complement(33681..34268)
                     /locus_tag="KQD82_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG4380111.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(34334..35368)
                     /locus_tag="KQD82_001638"
     CDS             complement(34334..35368)
                     /locus_tag="KQD82_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG4380112.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVRNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELVSPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLIDCSLKNKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKSGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            complement(35361..35738)
                     /locus_tag="KQD82_001639"
     CDS             complement(35361..35738)
                     /locus_tag="KQD82_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380113.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT        12055 a         6041 c         3825 g        14078 t
ORIGIN      
        1 taatcatgtt gaataataac atatgattaa tggaattaca atattttata acacaaaact
       61 aactaatata acaaattacc atcagtctat ttattcaaac ttctaactat ataatataca
      121 tttatttata ttaacaatgc aaacgatacc attatttgta attaagaatt ttggaattat
      181 tgattacaaa gtttttacat tctcatctca cataagtaat atttttatca aaaaattaat
      241 attattaaac tttataaatt acttatctct tgatataata gtatacaaaa caaaccatat
      301 ttcccactga aactttgttc aacactagct gtgtcaacgt tttcacagga aatttgagta
      361 tataatttat tggaggttga tatgtacaaa ttaatcgcac tagacattga tggaacaata
      421 ttaaatacac aaaaaaggat tactcctgaa gtctttgagt ccattcagga ggcaaaaagg
      481 gctggggcaa aagtagttat aacaacaggc agacctcttc ctggtgtaaa agaacttcta
      541 aatcaactaa atttaacaga tgaaggtgat tatgttatat gctttaatgg agcaattatt
      601 caagaagtta aaagtgagaa aatcatacat gatgtagaaa tgagtttaga tgattttgat
      661 tttatatata ataacgtttg taaaaaatat aagactaaaa tacacataaa tacaatgaca
      721 aatttaatta caccaaatga aacgcctggc aaatatacac ttcatgaagc gaaattaaat
      781 aatatagaag tcaaatatat acaaaaagat aaaattgatg aatctattaa aatctgtaaa
      841 attatgatag ttgatgaacc agaaagattg gaagaaatca tacagcaact acctaaaaat
      901 ttattcaata aatatactat agtaaggtct gctccttttt atctagaatt tttaggtaaa
      961 actactaata aaggaactgc tcttaaaaca ttatgtatga atctaaatat acctatagaa
     1021 aatgctattg ctgttggcga tgaagaaaat gaccaacata tgataaagta tgcaggtctt
     1081 ggtgtggcta tggggaatgc acgtaatagt ataaaggaaa ttgcagatta tgttactgat
     1141 actaataatg aaaatggagt tgcaaaggtt ataaataaat atatacttaa taaggctatc
     1201 taatcatcta aaaaaaacta aaaatatacc tttagattta aatacttttc acatccctac
     1261 tctatactaa tttatatagt atggatatta cctatcattt ttatctaaag gtcattttta
     1321 gtttttcata tatgtatatc tatgatagta ccttttgttt gagtttaaat attttatatg
     1381 attctactat tgcctcatta atatctaaac aatatatatc ttcatcatca gataatcccg
     1441 cttcgtttgt ccaagcatca aaatcactct tcgtccagaa gaaattcatt atatttcagc
     1501 aactagcagc ccaatttaga tgcttatcaa gatttatatg aagcactctt aagtttggat
     1561 tattcacata ctcaatcttt tcatttttta taattacttc tatttcctta cctgtagcac
     1621 tacaaataga gtttattttt atatcttggt tgaatgtgca agaggttcct atagcatcta
     1681 tagcacacat tgcactgaaa cttctattat ctctcaaagt tactttatga tttgttggca
     1741 atgctgatac aggatatata aaatttatac tattcccatc aacaaccatt atatttttat
     1801 aaataaaata ttgtagtgtc ctttctatat attctttttc tatattcaac tttttactta
     1861 catattcagt cacttcttga agtgtaacac tagaaccttt atctattatc atatccataa
     1921 tagagattct tattttctgt tgtgtattat tcaaactatt gtatttttct atttgactca
     1981 tgtctactcc tctttaaact tcataacact ttgataaagt atcagttaat ttgtttttgt
     2041 actcttcata tttttggctt cctctaattc taggtcttgg aatatctata tttatatcat
     2101 caataattct tcctttatct ctcgacatta ctataactct atcactcata tatacagctt
     2161 catcaacatc atgagttacc attaatgcag ttattttctt ctttatccat attcgttcta
     2221 gctcttcctg taaattttgt ctcatttgaa aatctacagc tcctaatggt tcatccatta
     2281 gtaatacctc tggattacat gctaaagctc ttatcatggc cactctttgt ttcattcctc
     2341 cagataattg atgcggaaac agtttttctt ttcctttcaa gtctgacata accaaaagtt
     2401 cttctagcat ttcttctctt ttttcttttg gtattttctg ctgtttcata ggaaatagaa
     2461 cattacctct tatagttttc caaggaaata aagcataatt ttgaaatata aaagctctat
     2521 ctatgcccgg ttttttaact cttttatcat ttataaaaac atctccacgt tctgattttt
     2581 gaaacccacc tattatagta agtaaagttg tttttccaca acctgatggt cctaaaagac
     2641 agacaaattg tccatcatct atatccatac ttatatcttc taaaaccttt gtatgtactc
     2701 tattatttac aaatgtctta tttatatccc ttacacttaa cttcattaat ctgcaaacct
     2761 ccatctacaa aggattttat ttataaactt taatacccta tctattataa atcctattaa
     2821 agctgcaaat atcataagcc caattaatac atctgtatgc attaatgttt gagcttgtac
     2881 cattgagtaa ccgagaccgg cactcgtagc tataaactcg gctcagataa cagacatcca
     2941 tcctgtactt atagcaatcc ttgaaccagt tagtatatct ggaagagatg cgggaacaat
     3001 tacatttgta aatatcacaa ttggcgatgc tcccatactt ctagctgcat tataataatc
     3061 ctttgatatt gctcttactc cttgaatagt atttaaaatt atagggaaaa cccctgaaaa
     3121 agctattaaa aatattgttg gaccatctcc tataccaaac catactatag taagaggaac
     3181 ccatgccatt attggaactt gccttacaga atctactact ggagataaaa ccctttcaaa
     3241 agttttagaa aatcccataa taaatcctat tggaagccca aacaataatg catacataaa
     3301 acctttaagt actctctgca atgttataaa aagattagtg actgtctctg catctgtcac
     3361 acagaaaaag aaacctttta ttacatctac tggcattgga agtaacaaag aactccctat
     3421 atcttttgct gcaaaatgcc agactaaaaa tataataact aatagtatta ttttatataa
     3481 aatatccata ctaccatcag tttttttctt agttaatcta tcgcttttat ctttagaaat
     3541 tttaatttcc aattgcttat ttcctccata tctatatttc ttatattcct gtactctttt
     3601 aatactttct taaatgtagc tttattcttt aaagcatcaa cgcttttatt gtaaagtgtt
     3661 ataaaatcac tatatgcttt acttttttta aactctttat ttacaactaa tacataggta
     3721 tcataatctc ctaatttagt tgtagatttt cttttaccct ctaacccaat tgactttatg
     3781 caatctatta caacaccatc tactaaatta ctttctaatc catatgctag tgcagtccct
     3841 aataagggag ctacttccac attcttgtag tattttttaa ccaattctga ctggtaatct
     3901 ctattttgaa tgactcctat cttttcagga ttattatcac ttaatagaaa tatatcagaa
     3961 ttttgaacta cagtaccaac aatttcaaat ccactattta catttacata ttgttttgca
     4021 gcatctttac atatgattgc tatatctatt tcctctgaac tcagagccca ctgcattgta
     4081 cttgctcagc aatcattaat tgtaaaaata tccattatat cacttacttt tatattttca
     4141 aagtagtctc tatttaccat ataatttatt acaaaaccag aggtatcatc agatacccct
     4201 attcttaatt ttccttttat ttcttcttta tcttcaaaat taaaaaatat tataaatata
     4261 cttcccaaaa taatgataaa aagtattatt atatttttca tattctccat ttatttataa
     4321 tacctttgct ttccagtctt cataactcat accttcagga aactctgggt taactttttc
     4381 ttttataaat ttgtcaaaat catcagctcc acaagagtcc aataagcttg tatcaatcgt
     4441 atcttcatat ttaggtatat catctaaagc ctttaaatct ttatacattt gtaaattatt
     4501 tttatattct tcacctgtaa ctttccaagt aagagttcta ccttctccaa cagtcttctt
     4561 atagatagtt ctaagtgcaa cttcttcttc tacattataa tattttgcaa atattttagc
     4621 tgcttcagct ggatttgtat atatatattc tattgctttt gtatgtgcta gaaccaattt
     4681 ttttgctaaa tctggatgtt cttttataaa gtttttgttt aatgagaatg aacaacagtt
     4741 atattctttg ccattagctt tttctttata ttgagtagat gctattattt ttcctgcacc
     4801 gtcattttca gctacagaac cccaagggtc acaagatgta aaagcttgaa ttttcccagt
     4861 tttaagagct aaatatttat ctttgtctga atccatattt acaacttcat attttgatgg
     4921 gtcagttggt agacctgttt ctggaccata atcacttgtc cataagaaat cactcatatc
     4981 tgttgcaatt tttttaccta ttaaatcttt aggttctttt atatcttttg aaactattaa
     5041 atattcagaa ccaccagtat ggttgttagc tgctattgtt attggcgaac ccttaggtat
     5101 tgctccaact aaccccttag ttcctatgta acctgcatcc atttttcctg ctgccatagc
     5161 ttcaggaact ttaccatttc caacagtttt tacattaaga cctaaatctt tatatattcc
     5221 tgccgattct gctactggtc ccgcagtcat atggtcacaa ttataataac ctactgttat
     5281 tgtataatca tcttttccag tttttgcttc ttctttttct tttggtttac tactacatcc
     5341 tgttaatata gatgtaccca ttacaccaaa taagattaat gcttgaatct tcttagataa
     5401 tttcatataa atttcccacc tttatgtatt atttttagca acatttgtcc atatcttcgc
     5461 caaataaatc aacaaatttt ttaccctcta ctctgtaaat agcatatcct acattaccat
     5521 ttttagaccc tgatacaatt acttctattg gtgctttatc atcaatattt ttaaaggtta
     5581 tcgtttgatt ctctataggt gctggtattg gctttgttat atagtggcta tttttatcag
     5641 aaactacgac taccatttca tttgaattat tgcttttttt atatataact actaaatctt
     5701 tttttccatc attatttaag tcttcttcag cacaagttat tatgtcttct ttataatttt
     5761 ttctaaaata ttctaatgtt tctttattca catttggaat agaagcatct tctttgatgt
     5821 tatcatcaac tatttttgat aaaatgatta acacaactac tatacaggat attataacta
     5881 tctttttaag catgatttcc tatgacctct ttcatctttg gtattattga tttaaatgca
     5941 aataaaataa ttaaaatagt acatacatac tcaaaactat caggtatatt taacattagt
     6001 ttatttgcca actgtattga agaatttgtt gcatctaaat ttactgagtt attattcact
     6061 aaaagtaaat ttgttatata tccaattatt attgaagaca gtgtcaaagt tacagaatat
     6121 ataataacag ttctttttcc aataagttta aacatactta taagttctgg tagatttgta
     6181 gctgctccag ctattagaaa tgttatcgct ataccaggag atgctccact tgcaacaagg
     6241 gctgctataa acggtatatg tcctacagca catacataca taacacatgc aagtattgct
     6301 atacttccaa gagatagcaa acctggattt cctaaatatt tttgaattat actatttgga
     6361 aaaatagttg ttataaatcc tgcaaaaacc atacccatta ctacatattt actaatagta
     6421 agggctaatt caccaaaaga ccatttaaga ccatcaatca tatttcccat aaaacttctt
     6481 ttttcttctt ctaattctat tacttcttcc tctgaatcat tatgaagtga taattcttct
     6541 tttccaaaac aatttcctaa aaatcctact aaaaatggta ctaaaaaacc agctattaga
     6601 taaatgattg taactttagg acctaaaaga cctagacaaa gaactacagc tattggattt
     6661 attattggag ttgatgtcat aaaagcaagt actggtccta aatatgctcc tgaataatac
     6721 atacttattc caagtggtat tactccacaa ctacatattg gtaaaaacat tcctgagata
     6781 gtacttttaa ttaaagaaga aaatttctta tttccaagtt gtttttgaaa ccttcttgga
     6841 gttaaaacat tgtgaagtac acctgccaat ataaaactta tcataagcca tcctgatgct
     6901 ccattcagtg taactataga agctactata gtatctttaa ctccatttaa aacaatttct
     6961 aatatattca tttttgatac tcctaatttt taaactttta attattgttt atagcttcta
     7021 ctattgccct ttcaattgta tctttattta atctatcata tttctttttc tgatttatta
     7081 ttaaagttcc ttttgttata atcccatatt tttttatata cgaaaaatct ttccctgctt
     7141 gatataattt tatttccaat ttatcagcat atttttctga catttttttt ataaacactc
     7201 catgttcatc acaagatggt caagtgttta taaattcaac taaaatttta gacatttgtt
     7261 aatcccctac tttaaacaat ttatctttta cttaaaataa tccacagaaa ataaattttt
     7321 ctatggattg tatcctatac taatttataa cgttataact tggtccacac ctgcactgcc
     7381 taaacaagca tataggtttg ggaaacatcc aattgtagct ccatcaacca aattattctc
     7441 tatacctctt tctatagcac attggtcaca agccattatt atcatatcag ttttttcatg
     7501 taatttactt agtctctcgc ctacaggatt acctttagat aaaaataagg tattatccat
     7561 aaagaaaaac atccctagaa cgtctactcc atgagttcct tgttcaagtt gtggtattat
     7621 cattttgtct agaattttat gtgtattagt actactaaaa atataagcta ctttcatttt
     7681 tattccccct atacttttta tattttacaa aattttacag atacaattat attataagtt
     7741 tattcttatt tatctgtaaa atagatatta actatattat taaaagtgta tatagtattt
     7801 atctattttt attaaattat tgattgattt ttttctattt ttattaattt taatgaataa
     7861 aaaatagact ccttaaattt ataaaacaaa tttaaggagt ctattttaat taagtttatt
     7921 tcaatataaa atttatatat ttctcaaatt cttcatactt atatcgagag ctttaacaga
     7981 atgtgtaagc gctcctacag aaacaacatc cacaccaatt tttcctatat cttctatagt
     8041 atctaaattt acatttccgg aaaattctac aatggctctt ccatttataa tatctactgc
     8101 ttgttttgcc atttccaaac tcatattatc caacattatt atatctgcat tagcttctat
     8161 tgcttctttt accatgtcta aagtttccac ttccacttct atctttctaa caaaagaagt
     8221 attttttcta gctaattcaa ctgccttctt aacactacca gcagccccaa tatggttgtc
     8281 tttaagcaat attccatcag ataaattaaa tctatgattg catcctccac ctactttaac
     8341 agaatactta tctaatattc taagattagg tatcgttttt ctactatcta ataattttgt
     8401 atttgtacct tcaagctttt caacatacct acttgtaata gtagctatac cactcattct
     8461 ctgtaaataa ttcaatgcta cacgctctcc tacaagtaag tttctagtac tacctgttaa
     8521 tacagctatc ttttcacgat tctttactct atcaccatca tttttatata attttacatc
     8581 tacatcacca agtatctcaa aaactctctt aaatacacct aagccagcta ttataccatc
     8641 ttgtttacaa attaaatcta cagtagattt agaattttca tccacaatag aattagttgt
     8701 tatatcttca cttggaacat cttctatcaa agcatcctgt atcattctat caataatcaa
     8761 ataattcatc gccaaaatcc tcacttgttc tttcaatttc ttttatgact aatattttat
     8821 tttctttttt tacctgttct atatctatat ccatatctgg tacatctata aacttaactt
     8881 ttacattatc tataactgag tttatatttt tagctgctct ttttgaaaat acaagccctt
     8941 caagtaatga attacttgcc aatctattgg ctccatgaac acctgtacaa ctaatttcac
     9001 caactgcata taaattttcc atagatgttt ttgaatccaa atctacatgt atcccgccca
     9061 taaaataatg ttgtgcagga gataccttta tacattcttt cgttatgtct gttcctctag
     9121 ctaaacattc attataaata aaagaaaatc tattaattaa atattcacta tctaaatgtg
     9181 ttgcatctaa atacacataa ggtaatttat ctttttccat ttgctcaaaa atagcttttg
     9241 aaactacatc tctaggaaga agttcatcca caaatctttc tttattttta tttaaaagtt
     9301 tagctccctc tcctcttaat gattcagata taagcattct cctaccttca ttattttctt
     9361 cataaaaggc tgtaggatgt atttgaatat attctaaatt ttctattttt atattatttc
     9421 ttaaagctat tgcaattcca tcacctgtaa gatgtctttg attagtagaa tttttaaaaa
     9481 gcccaccaat tccacctgta gctaaaacta ctatttttga aaaaacatga atctcttttt
     9541 tagatttaaa aattctagct cctatacatt tattgccatc ttttaatata tctaaaagat
     9601 aggcatcttc tataagagtt atattttctc tagttttagc ttcttttaat aaagttttaa
     9661 aaacaacttc tccagtatta tccttactat gaactattcg atttacacta tgtgctcctt
     9721 ctcttgtgta atctatctct ccattttctt tgtctaaagg cataccatat tctactatct
     9781 gccctacatt ttctatagat tcatctgcaa gtacttgaac tgcttcaact ctatttttat
     9841 actgcccagc cttcattgta tcttctacaa aggattctat atcatcaata tttctagcag
     9901 ttgaaattcc accttgtgca agatatgtat tgttgttttc aattgttgat ttcgatacta
     9961 ctagcacatt taaactttta tctaaattta atgcacagta taatcctgaa actccacttc
    10021 caactattaa aacatcttgt tctaaattca taagtctatc cctctatcct tctgctaatt
    10081 tgtgcatgtt aagcagtgaa tttaaagcct tttccatgat ttcatcttct acaataactt
    10141 cattttccat atttttaagt gtactatata aattttctaa tgttgtcttt ttcatatctg
    10201 tacatagtat ttttccacca ggaatataaa agtttttatt aggattattt ttctttaatt
    10261 catgtaatat accttcttca gttgctatta taaagtcttt gtttttacta ttagtagcat
    10321 attttattat tcctgatgta ctacctacat aatctcctaa atctcttatt tcttttttac
    10381 attctggatg tactaatact tctgcatttt tatacttatc ttttaactca attatttctt
    10441 cagctcttat attgttatgg tatttacaat atccgtccca aaatataaag tttttatctg
    10501 gaaattgttc tgcaatatat cctcctaaat ttttatctgg aaggaatata atttctttat
    10561 tatctatgtt ttctagtatt ttaattgcac tagaagatgt aacagaaaca tcacaatgtg
    10621 ccttaacttt agctgtagag tttatataac atacaaccaa agcatttgga tactgttgtt
    10681 ttaattttat cacgccttct tcatctacca tatctgccat agcacaccca gcgttactta
    10741 ctggcatcaa tacagttttt tcaggtgata atatcttagc actttctccc ataaacctta
    10801 ctccacaaaa tacaaccact tcttctttac aatctctagc aatctcactt aaataatatg
    10861 agtcaccaac tgcatctgct aattcttgta tttctggtgg ttgataaaaa tgggcaagta
    10921 ttattgcatt tttttctttc tttagctctt tgatttgata tgttaaatct ttatccataa
    10981 ttgcaccgct ttctatatgt actagtgtat atacacctgt aaactcatta tatcacttga
    11041 ctattctttc ttcaagaatt ttaaacctct taatattgtt aagatataga catttatatt
    11101 ctcaattgat tttccttttt tctccatcta tttaaactaa tttttatatt ttttaatttt
    11161 tttgtaaaat attatcagaa ttttgtttat attctttatt gctataacat tgaaaatatt
    11221 accaaatacc tgtatactaa gtacatggtt atgtatatgt atttatttct gaaattatat
    11281 tagttgtaat actttataga atttgtaggt tgtccagaat taaattcaat ataaatctac
    11341 acatttagat aatatgtata caaagaccaa aaaattttat aaatggggtg atattatgac
    11401 aacattctta atcggcttgg ctatattact aataggaggt gctttatacg gtgcttattg
    11461 tgaaaaagta tttggcccag atgacagaaa aacacctgca cttgctcaaa gtgatggagt
    11521 agactatgtc ccaatgaaaa aatggaaaaa tagtctaatt gagttgctta atatagctgg
    11581 tacaggacca attctaggac ctatccaagg tatattgttt ggtccaatag cattcatact
    11641 aatacctatt ggctgtgtat ttggaggagc tttgcatgac tatatgagtg gaatgattag
    11701 tataagagaa aaaggagcac aaatgccttc tcttatcagt agatttttag gtaataaagt
    11761 atttcaagtc tataatatat tcctttgtct tttaatgctt ttagttggtg cagtttttat
    11821 ttatacacct ggggatttag ttgttactca aattttaaat atgaaatcga ctataaataa
    11881 tccagttgtc tggatagttt atggacttat ctttctatat tacttgtgtg caacactatt
    11941 tccaattgat aaaatcatag gaaaggtata tccaatattt ggagctattc tacttttatc
    12001 tgctgctggt gttggaattg gaatctttac acaaggttat gatttagcta atttaagtct
    12061 tgcaaattgg aaaggtattc atcctgatgg tattcctctt attcctacat tctttgttac
    12121 agttgcttgt ggtattgttt ctggattcca ctcaactcaa gctactctta tagcaagatc
    12181 agtttcaaat gaaaaagaag gaaaaactac tttttataat atgatgattt tagaaggttt
    12241 aattgcaatg atttgggctg cagctgctat gggtatttac aataaaggaa tacctaagga
    12301 attagtaggt tctcctgatg taataggttt ggttgcaaga gatttacttg gttctattgg
    12361 tggtatcata gctataatag gtgtaattgt tttaccaata acttctggag atacagcttt
    12421 aagatcttta agattaatgc ttgcagatta ctttcattat gaccaaaagg aaaaaaaaca
    12481 tcgtgttata ttgtcaatct gtatatttat tcctgtcata gccatcctta tatttgcaaa
    12541 gcttagtgct tctggattta atatattgtg gagatacttc tcttggagta atcaaactat
    12601 tgccatattt gcatttgcaa tgattacagt ttaccttata ataaaagaaa agaattatat
    12661 tataagctta attcctggta tgttttattc ctttgttata ttcagttata tatttaatgc
    12721 tcaaattgga tttaacttaa acataaatat atcatatgtt ttagctgcca tatttactgt
    12781 attatatgct attttaactg tacgctctgg tagaaagtta aaaagcaaag cagatactaa
    12841 gttggctgac taaatctaaa taaaataata cttagaataa attttattaa aaaatgataa
    12901 aaaagtaggc aatctcaagt tagatttgcc tcttttttat ttaatacagt aataaatact
    12961 taagtattta ctccttattg gaatatttat cctaaatctc taatataaat aaataaaagt
    13021 tttaaaataa tctattcgtt tatatactta aaataaattt tttggaggaa tacttatgaa
    13081 taataaatta catgaacttg aaaaaaatct accagaaaca tcttgttctt tttgcacaca
    13141 tctttcattt aaggggccta gcctagacta tagatatgat attagatgtg ttatttcaga
    13201 tacaaagccc gattttagag gatgttgtga atattttgaa ccagaatata cagaattaaa
    13261 tacaggagat ttagacaatc tatacataaa ctttcttgag acatgcctca aggtaaatta
    13321 taatgattac atcaattcta tgtattggaa gttatttaaa gaaagaaccc tactagaaaa
    13381 caattttaaa tgttctatat gtggatcaac tgaaaatgtt gatgtgtatc atgttaataa
    13441 aaatttagga agggaaactt ccaaagatgt agttgttaaa tgtgataaat gtaattgtaa
    13501 ataagtgtat aaaatttaga taacttcaca taataagttt aaataaaact taggaggtta
    13561 tttatgaaaa ataataattt aaattgtgct gctactaatt gtgcttataa tactagtgga
    13621 tattgttatg ctggtagtat aaaagtagat ggaatgcaag caactactac agaaaatact
    13681 tattgtgctt cttttgaaga caaatataca tctggtataa ctagtcgttc taatgaaact
    13741 aaccaagttg atacagataa cattcattgt gaagctgtaa aatgtaaata caataaaaat
    13801 gaactttgca aagctgaaaa agttcatata aatgaaagaa atgcaagttg tgaaactttt
    13861 gaaatgaaat aaatttcttt ttatttagtt ttatagtatt tatacaaagc aaaattgaac
    13921 ctatcaaata gatgtcttta ttaatgatgt ctatttatag gttctttttt aaaacaattc
    13981 ataatttata tataaaaata aattattatt ttaatatatt acctagaagt aaatttaaca
    14041 cccagaatat ccataaggaa cataaatatt ggaactccaa ttagtaatcc ccatacacct
    14101 aaataatgct ctcctacaag taaaataata aaaacaaaac aaactggaag tttagttcta
    14161 ttcgacatca acttagggtt taatatgtat gtttctactg catgcactat acaaatcata
    14221 agtaaaactc caaaaacctt tgttattcct cctgtattaa atgctattac agttaatgga
    14281 atcaaagaca ctataactcc tgctactgga ataagaccca acacaaaaat cataaatcct
    14341 aaagcccata tttgagggaa tcccatgaaa cctaagaaaa tcatagaaac aacagaattt
    14401 ataaaggcaa tcataacttg tactttcatt acttcaccaa aatttttaac aaagctcttt
    14461 ccaaaaaata caagcgaatt ataaataaaa gaaatcttac tatcagaaag tttatctcca
    14521 aaatttttaa tttcattttt ttcaagtaaa aggagtaaac tcagtaaaaa tgccaataat
    14581 atatttactc caaaacttcc aacttttgtt gctgttgatg ctataagtag ccctgcttgt
    14641 gaaatatatt tattaaaatc cagctttgag acaataggat acaattttgg tcctaaagat
    14701 tgagcaaatt gatttacatc aaaatcacta aaaaccctac tcaactctgt aagttgaact
    14761 ataattattg gtacaactgc atagctaatc aatactaaca atatagcaaa tagtgcatat
    14821 aacactataa ttatcaatga atctggaata tttaaagaag aaaacttctt aattcgtttt
    14881 cttgtggcta caagcaagtt atagaaaata aaagtcataa taaatgttaa aagcaccata
    14941 tttaacatat accaaaatgt aattagaata attactacga cagatataga aacaatttta
    15001 tattttgaac tgtcacgtgc caatcctttg ttatcttcca tagtttaaat cctccccaaa
    15061 tttttatgac tttataatga ctgccctttt tattttaatc tcaaaaaaca agtagaaaaa
    15121 tactaaaaat aacttattaa ttttcataaa acaatattaa aatttagaga agatacattt
    15181 taaataatct tacttatact gaaatctgct ctacatcata tatttaattt attattatct
    15241 atctcatata gcaattcgta tatctctgga tattcaaaaa acaaaccctc ttttagtaat
    15301 tgtcttattt catctgtgct tacccacttt atatcactta cttcttcctc ctgtaaaata
    15361 gctttggata tatcatattc ttttttaact aagtatacat cccaaagagt atcttcatga
    15421 atcatacttc taaaaacttt catctcatct ttagttatat ctatccctat ttcttccttt
    15481 gcttcgcgaa ttgctccttc taaactgtct tccccagaaa caatacatcc agttgtcatt
    15541 ccccacatat ttggcaatgt ctttttactt ttagaccttt tctgaattaa aatttgagag
    15601 ttactattta aaatccacac ttcaactgcc aaatggtaat aaccttcttc aatagaatta
    15661 cctcttttta taacatttcc tgttttaatt ccatcagcat tatataaatc ccataattcc
    15721 atttcaaaat ctccttgact ttactataaa ttagataaac tacaaatata ttttagcata
    15781 attaattctg ctcatttttt attacaatac ttgatttaca tttatgttca ttgcttttct
    15841 tttccttcca ttctcttata tcatctataa taaatctatg tttcaacaat tttgtgtcaa
    15901 atttaaatat tctaaacact atttgtaata catatcctaa acctaaagca cttacaagcg
    15961 taccaatacc tacagaacct cctaataacc acccaacaac taatgctagt atttcaagaa
    16021 ttgttctaac aagacgtaca gatttattag tttttttctg aagagcaatc ataagcccat
    16081 ctctcggtcc acttcctaaa caaacaccta agtatagaaa acttgctata gctgccaaaa
    16141 taattcctat aacaaccata aacacaccta tataagtgtt gcttgcatga ggtatcactc
    16201 cagaaaatat tactaaatca ataaaaattc ctataaatgt catgttgagt aaagtacccc
    16261 atcctatatt ttcaccaaaa actgcatcca atataacaat tacaactcct accattatag
    16321 aagcctgacc tatagttatg cctagcttta tagataaacc ttggtgaagt acatcccaag
    16381 gcataagacc tacatgtgca ttaatcataa gcacagaaga aattgcaaat ataaaaaaac
    16441 ctgaaaataa tcttatcata ctttttaaac ttatttttcc catcaaaaat cactcctcat
    16501 aataataata acaataaata ccaataaaat atatatataa atacgaaatt ttacagaatt
    16561 atttattagg ttaagataaa attttaatta taaaaagtgg caatccaaat tttatacatt
    16621 ttcacctata ctctaagctt tcgtgtataa aaaacggata taccactttt ttagtatata
    16681 aacattttta gtttttctta ttaattttca ctataaattc tagcttcttc ttctccattt
    16741 agtactctta aagttccaaa agcaagtgat tccaactcat tttctcctgc cataatttct
    16801 actggtgcta taaactcaac tttcttttta accattgaag taaacatttc agaatatgca
    16861 attccacctg ttataattat agcatctacc tttccatcta ctacagttgc taactctcca
    16921 ataccttttg ctaactgata agccattgct tcataaataa tttttgcttc ttcatttcct
    16981 tctgctatca ttttttcaac ttctctagca tctacagtat ttaggtatga aactatacca
    17041 cctttacctc ttatcttttt agtcatttct ctttctgaat attgacctga ataacaggca
    17101 gccactaatt ttgtagcagg tactcttcct gacctttctg gagaaaatgg tccttcatca
    17161 tcagatagca tatcaaccat tcttcctttt tcatgaagat aaatacttac tcctccacca
    17221 atgtgagcta ctattaagtt taagctctta taatcttttc ctttatcctt agcatatttt
    17281 aaagccattg ctctagtatt taatgcatgc cctgcacttg atctatccat tccacaaagt
    17341 ccagatattc tcgctacatc tataagctca tctactgcaa cagaatcata aatatatgag
    17401 ttgattccaa gaggttctga tattgcatgt gccacaacag cacctaaatt ggaagcgtgt
    17461 tcaagtactg gtctatgtct tagtacatca atcatttctt catttactaa atatgctcct
    17521 gattttactg gtggtaaaac tccacctctt cctactattg cactaagaga ttttaagtct
    17581 atactatttt ctttaagaat attcaaaact gcttccttac gcatttcaaa ctggtcttgt
    17641 atagtattat attttgcaat ctcttcagct ggatggtcta tcgtcttcac aagaatttgt
    17701 tcttctccat catatactgc tatttttgta gaagtagaac ctggatttat ggctaatatt
    17761 ctgtaagtca taattttctt cctttcacct taatttctat agtctattta tatctattat
    17821 aaactatttg ctaaccattt ccataccttc atggaaagct tttatgttta attcaacaaa
    17881 ttttggttta acattttcac ttataatttt ttcccaatca atagactcta attgcataga
    17941 tttaactaga catcctaata aaataacatt cattattttt gaatttccta gttcttcagc
    18001 tttatcagct gcatttataa cttttgcatt taatctactt aactctgcat ctatatcact
    18061 ttcttgatat tcagctcctc cgattaaaac agtcatagaa tctatcctat gattatttac
    18121 taaagctttt ccttctggtt ttaaataatt gaaccatctt aaagcttcca ttttttcaaa
    18181 agatactaat atatctgctc caccaatttc tataactgga gaatgaacac aatctccata
    18241 tctaacttgt gaggaaactg aacctcctct ttgactcatt ccatgtattt cactcatctt
    18301 tacatcatat ccagcttcca tcaatcccat agttaataat ttactagcaa gtatagttcc
    18361 ttgacctcct acacctacca aaagtatact cttagtcata ttatttttcc tccttaacta
    18421 ttgcttgttt tggacaaact tgtgcacata ctccacatcc tacacactga tttctatcta
    18481 tatttgataa cttattctct ttatcaaatg atagagctgg acatccagtt ttcaaacata
    18541 atttacatcc tatacattta tcatggtcaa ctttacactt agtcttaaat ggattattaa
    18601 attcttctat atcttcttta gagaattttt taagtacaca tggccatctt gttattatta
    18661 ctgatggttc atcttctata gctaaagccc aatctagtgc ttcatttact tcttttaaat
    18721 tattagggtt tataactctt acatgttcta ttccacaagc tttaactaat ccttctatat
    18781 caacttcttt agttttagca ccttgtaaag tatatcctga acctgggttt tcttggtgac
    18841 ctgtcattcc agttattcta ttatctaata taaccgaaat agaattacct ctattgtaaa
    18901 caacatctaa aagtccattt attcctgtat ggaagaatgt tgagtctcca agtacaccta
    18961 ccaatctttt ttcattatca tccttcatat taagaacttt ttgagctcca tgtgcagttc
    19021 caaaagcaga acccatacaa acaacatagt ctattccatt atatggagga gcaaatccaa
    19081 gagtataaca acctatatct ccaccaacta ttaaattttt tctctttcca agttcataga
    19141 agaatcctct atgtggacat cctgcacaga aacttggtgg tcttggtgtt actaattcag
    19201 atttgtattc tatagtatca tttgtttttc caaatatagc ttttcttaaa acatctggtg
    19261 tcatttctcc atatggtggt attacatctt ttccaataca atctattcca tatgctttta
    19321 tttgttcttc tataaatggg tcattttctt ctataacata tattttatca actttttctg
    19381 caaattcttt tattttttca taaggtaatg gatttgtaaa tcctagtttc atatatgatg
    19441 cattttttcc aaaaacttct ttggcaaaat tacaacacat accagatgct ataacaccta
    19501 tttttgtatc atttatttca tagtagttta aatcagtttc atttgagaat tttttaagaa
    19561 tttccattct ttcttcaaca acgccccttc ttatttgagc atttgctgga acagttacca
    19621 ttttcttagc atttttaaca tactctttaa tttcaacttc ttctctatca tagcactcca
    19681 ctaatccttt tgaatgacaa agtcttgttg taactctata taaaactggt gtatcgtatt
    19741 tttcactaac ttcaaaagct tcttttatca tatctttagc ttcttgacta gttgatggtt
    19801 caaataaagg tatctttgca aattttgcat acatcctatt gtcttgctca ttttgagatg
    19861 aatgcattcc cggctcatct gctgtaataa gtaccatacc accatttacc cctgtatatg
    19921 cataagtaaa tattggatca gctgcaacat tcaaaccaac atgtttcata gaagccattg
    19981 ttcttgctcc tgcaattgaa ccccctatag cagcctcaag tgctaccttt tcgtttggag
    20041 cccactctgc aactattgca tctttatatg ttgctatatt ttctaatatt tctgtacttg
    20101 gagtccctgg atatgcagat gcatatttaa caccagcctc ataggctcca cgtgcaattg
    20161 cttcattacc tgtcatcaat tgtttcatat aaaattccct ccatctacaa ttattaaatt
    20221 catagtccac ccaatttgtt caatgaaatt aattatttat ttttacttct aaactttcaa
    20281 gtgcttcttt tataatactt ggtgctttct tacatttatc ttcagaaact gcacaaactg
    20341 caaatcttaa gcccattttt aatggaactg caaataaatt atgtttagtc aattcttcac
    20401 atacttcttt tggattatca catggtatac ttacaaagaa acctgctata tatggaagaa
    20461 tttctaatcc tactctttca gcttctttta caaatacatc tgctcttcta gtaagcatgt
    20521 ttttgtatat ttctttttca tcctcatatt ctttgaattt tttagggtca tttacaatgt
    20581 tagataatac tgccatagca cttctattac aatttgacca atttgctcta catgaatgaa
    20641 cacatgagta atggaactct tcagctacat cttcacttga agatatacaa attatagctc
    20701 ccattctcat accataagct gtaaatcctt ttgacatact gaatccaacc aatacgagaa
    20761 cgttttcagg caaattagaa aatttcttaa aaaactttct acatacatca tcatcacctg
    20821 caaagtcaat atatgctgaa tctacgaaaa atattatctt tttgtcttta tcttttgcga
    20881 cttcttttga taagtctaat attttatccc actcatcatc tgcaacacta tatccagttg
    20941 gattatgcgc tggtgtgttt ataattgtaa agactctacc ttgtttttct gctatattta
    21001 caaatttttc tttaaacgac tcaaaattaa accttctatt ttcatcaaaa agttggtaat
    21061 taactacttt tctattagcc tcttcagcta tacttacata tggactccag aaccaatcag
    21121 atgttaatac ctcatctcct tcattagtat agttccatac agctaatttt attgcaccac
    21181 ttcctcctgg tgaagctaat actcttatat gtccttctgg taagtaatct ctgaaaaata
    21241 cttttttaac tgcttctaag taatctggtt gaccttcaag agctgcatat gcacctatct
    21301 cacagttatc taatacttta tattcttcat atacagtttt cattgttatc aattttcctg
    21361 aatcatccat tagagcgcct attgttgcat ttattacatt atccatacca aaagccttct
    21421 ctgctgcttg agctctgcca gatatgctaa atataacatc attttctttt ggccacattg
    21481 catgcttacc aaccatactt tgactcatac catcacccct aaaattgtat ttactcttac
    21541 attatatatt aagcaattac tatgccaata aatcccactt tatgtaaaat tgattttgtt
    21601 tagaattatt atttttttca ttattaaatt aagtgtttta actatttttc ttataaatat
    21661 atatgcctgt ctaaaaaaat aaaaatttgc aaaaaagtaa tgttaatttt tcttaacact
    21721 tgttttaaaa aattaacatt acttcaaaat aatttatatg taattatatt taaatatttt
    21781 tattgtattt cttccataaa gatgtcctgc ttattccaag aacttgtgca acttcatttt
    21841 tagaaagtcc ctgagcaaaa agcatactta ttattttttc ttcaacatac ttatttactt
    21901 cttttatatc tatacttatc ttaccatcat ttatcttact tatatttata gattcatctt
    21961 ggacttctac acttgctgtc gtttcagaat taaatttgtc tacttcagag ttaggaagta
    22021 attttttagt tttaaaatct ctatcttgtc tcaatttaat tttacctgtc acaacatatt
    22081 tttgagctat tgtctttaac tctcttacat ttccaggcca gctataatta tatagcttat
    22141 ccatttcatc tttagtcaaa acaaaatttt catctatact gtttattcca ttgagaccat
    22201 catcctttaa tacttcattt acaaaattgt taaacaaagg tattatatct tctcttctgt
    22261 ctcttaaagg aggtatattt atctctaaac tactcaatct ataaaataag tctgctctaa
    22321 atgtacccat aactattttt tcggttaaag attcattcgt tgctgctata atccttatat
    22381 ccagaggtat tatataatca gaaccaattc tcattatttg acgttcctct attactctta
    22441 atagttttgt ctgaatatta aaggatagac tatttatttc atctaaaaaa agagtccctc
    22501 catgagcaag ttcaaatagc cctctttttc caccctttct agcaccagta aatgcacctt
    22561 cttcatagcc aaaaagttca ctttcaagta aattttctgc tatagtagca caatttatgg
    22621 caacaaaagg cctatctttt cttttactta tattgtgtat actatgagct ataatttctt
    22681 taccagaccc actttctcca taaagtaaag ttgtataatc actttttcca atttttttag
    22741 cttcttcaat aaattcttta gttaatttat ctttaaataa aaaatcatca aaagtatatc
    22801 tagcatagag acctttttga ttcaagtcaa atcttatttt tctttctaag ttttgtaact
    22861 tggttatatc ttgtattatt cctaaaactc cataagtact attatcaact tttatcaatg
    22921 ttgtcctagt attaacaatt agattattta tatttcttat ctttctatct tctacgtcct
    22981 ctttttcatg taaacaatct aacatccact ccattttagg aaatacatct aagatgtatt
    23041 tattaagtgc acattttctc tctactttaa gcatgttttt tgcactttca ttgtacaaaa
    23101 ttatactgcc attactatct atagcaatta ctccatcttt tataccatca agtatatttc
    23161 gtaacacttc attattaaac ttttcttcac ctaatgtatc taataatttt ttacaatatt
    23221 ctacagcttc tcttatagat tcatcactag cagttgcaaa tacactatca attccatatt
    23281 gccttgcaaa agaacatgcc aaacctcctc ctacgattac aacttcatct ttttggtcta
    23341 tatattttac aactttactt cttatctcat acttagattc aaaccattct tcagttattt
    23401 cagtacttat gacatttctc cagccaacat agtcaaaaga aacttcattg tctccaagaa
    23461 tcaatactac ttttttagaa tatttctttg ctatttctat tgctcttaaa aggtcagttg
    23521 acttcatatt cattggtata acaggtacat ttactgtatc tattactaaa ctataacctc
    23581 cacctctacc aattatagct tgggcacctt tttcaacaag tatacgacct tgattttcca
    23641 ttaagtctag gttaagtatg tcgattatta tagttccatt ttctacatct tctctatata
    23701 attcttctat tctttctttt aattcaatat cagaggctat aattcctatt ttttttcgca
    23761 acaaaatcac ctcaatttta aattataatg tattgatttc tacctgcttt ttttgattta
    23821 tataggttct tatctgcatc ttcaattaat atttcaacat cttttctact atgacacctt
    23881 tttatactta ctccagcact tatagttata tagtttttta ttttagaata tatatgttca
    23941 ataccctcaa attcaagaag tttgaaaact cttttaatat ataattctat attatcaatc
    24001 tcttcaacac cgattgattc tatagaatct actaatatta aaatttcttc tcccccatat
    24061 ctgattatgt gttcggtaat atctataaat acacttctta taatatcagt taccttaact
    24121 ataactttat cccctgctag atgaccataa ttatcattat aacttttaaa acaatctata
    24181 tcgaccagta ttgcaaccat atttttgtct ctggttccat taaataaatc caactgctgt
    24241 tccaaatatc ttctattata tagtttagta agtccatctt ttaaaatatc attttctaat
    24301 ttcttattat catccactag taatcttaaa ctctcttgtt tttgtctgag ttcatattgt
    24361 ttgtgaattg cttgagatgt tttataggaa ttattttttt cccacctatc agaataccaa
    24421 tccatacttt tataatattc tattgccagt ttaaaatctt tttttagctc atatccattt
    24481 gctattagcc cataaaatat agatattttt ctaagattca atgaactctt tttaactaaa
    24541 tttaaacctt ttatataaca acttatacct tcatctatat taccaaattt tatatataaa
    24601 tctccataca acttatatat gtaatctagt ttagaattat atataaaaac atctccaaaa
    24661 cttttatata tattttttga taaaattaaa taggtgttta ccttttcaaa actacaatta
    24721 tctttcttta actcaatttc agctgaatat aaaaatataa atggatataa ataatcctta
    24781 tatacacctt caacaacttt cattaatttt atagtcttta ataaaataac ttctgcttct
    24841 ttattcatat cttcttttaa atatgcccat ataaggtcta tttttataga cactctttca
    24901 gttactgata ttgagttatt actgtgtgca acatctaata cattatttat gtattttata
    24961 gcttctacat tatttccact ctctaaataa cacaaactta ttatacattt acaaaaagcc
    25021 tctatagagt ataaattatt ttcttgagcc aattttagac attcaattaa gtattccata
    25081 ctttttataa agttattaaa taaatataga taaactctcc caaatatata atttgcccta
    25141 cttacaagag ctttattttt taaagtttta aaaaaagaca cttcttctac taaagctatt
    25201 acatccgttt tggtctgata taaagcactt atttctacac ataaactcat atttaacaat
    25261 accaacaact catttaaatg attttgtctt ataatttttt ttgctgagaa ataaaaatta
    25321 tttaccttat catattttct tatagaaata gctgataaag taagataaat ataaatttta
    25381 gatattatat ctgaatcatc ttttaatgac aattttttaa tatactttaa tgactctatc
    25441 aaatagttgt atgatgcttc tctatctttt tttatatagt ttataagact aattaaatat
    25501 aaagtataga ctctactatt atcttctaat tttgtattta aaaggtcttt gtgataatcc
    25561 aagatgtagt ttaaatctgt caaatttaga tagaatttag tcaacatatc gtattctatc
    25621 ttgtttatat ttctctttaa atcttctatt aaactccttt tcaagtaaat caccaatcct
    25681 atttcaacag ttaaaaattc ttaaaatcat gataatgcaa acatctattt ctaccacttt
    25741 cctttgcttt atataaagct ttatcagcat tttcaattat ttcttttata tctatactac
    25801 tatttttaca tgtatcaatg ccaatactaa ctgtcaaatt attgtttaaa aatttcttta
    25861 caatcctaca taattttctt ccaaatattt tacttttttt ataatcttta catacagata
    25921 atatcagaaa ttcttctcca ccatacctta tcataagcat gtcttttttt agatattttt
    25981 ttattattat agttatattt ttaagagcaa tatctccatt gtagtgtcca taagtatcat
    26041 tgtattcttt aaagtaatct acatctatca taaatgctgt gcaatattca ctaacttctt
    26101 gtttttctaa aaaatcgttc aaaaaagttc tattatatgc acttgtcaaa tggtcaacat
    26161 actttttttt atctaaatca agttttactt ttctcatgtt atttatttct ttttctatat
    26221 actcatgtct ataatttaag gaatctatat aatttctatt tcgagctttt atatacatca
    26281 tttttaactc ataatacatt ttaaagtatt tcatactcat ttcataattt tgaatctttt
    26341 caaaacacaa atatatactc ttatatgcta acttcacata attataattt ttagcatttt
    26401 tagatttttc aaggagtttt ttatttgtgt ttaaggcatt gtcataatat ccaaagatat
    26461 aatatatctt tgcctggtga taaattattt ctaaatcata tattatatat tcttctatat
    26521 tttttatcat atttaatatg tttttagctc tatctaaata gcatattgat tgttcaaaag
    26581 atttttcatt actttttgca taatacatgg ctaaacttat aaataaatca aacttctcta
    26641 cttcttttaa gctatgattt ttaatttttt ctagatatac ttcagatttt gctaaattat
    26701 ttattgcgac aggataattt ctagtcttta ttaatatttt agttatgttg tttgatgctc
    26761 tatatttttg aattatattt acatttctat actttttatt gcttattact tcattgaaat
    26821 attccatgga acattcataa ttttcaaacg aacattcaca ctcacctatc tttatattac
    26881 atagtgaaga tacttgcata tcctttagta aatctgcata ttttcttgct tttaaaaaat
    26941 ttaccttagc aattagagca tcatccaaaa acaaaaaata tattttacca ataataaaaa
    27001 aatatatttt tttacaatta aaatcgttaa tttcttttat ctttatatac aacgtttcta
    27061 tctctttaat tattacagaa gattctatac acttacttgc cttaatgtaa ataagttgtg
    27121 atttaaaaat aatttcttca attagaaaat tattttcatc acaaataaga ttaaatttat
    27181 ccatatataa atttgcctta tttacatctt cattttttaa acttaaaata gctatttgtg
    27241 aataaaatcg tgctaaaaat ttataatctt tgggtaagca tttatcaata tatttacagg
    27301 aaatcaaaat atactctttc attttttcac taaacccttg aataccatat aataaagcta
    27361 gcaaaaaatt tgattttaat aacattaaac tattttgttc agttttatag caatcaatta
    27421 aattctttac tacatcaatt ttattttcaa taaaatcgtt attagtcaat aatctttcat
    27481 tcaaggttag tttaaacatt gaatctaaaa ttgtatcatc tatctcttcc aaatatttac
    27541 tatatattgt aactcacctc cgacttacat tatctcataa atttagtaat tttccatact
    27601 atttttgaaa aaagttgatg tctcaattta gaaatttctc ttaaattaag acatacttta
    27661 cactaaacta tacaattttc tatctgaact atataatcct gacaattttc gcaaattatc
    27721 acacttacaa tataactatt atcaacttta tttgcccttg atagtttatt aaaagatagt
    27781 cctttataat caacagatat attgcttatt acatattctc catctttact gaaaatataa
    27841 aagtgatatt ctaaaaaagg aaagaatttt ttcttttctt ttactccttc taaacataat
    27901 ggacatgctt cttgataata ttctacatca aaatcaactt cttctgcttc attaagtata
    27961 tcaattattt ttgaaatact tactccttga actggatttt cttcatcatt tattaaagca
    28021 ctacagttct cctcttttaa taaatattcc ttatcatcat atataaactt atataaactc
    28081 atattatatc ctccttataa ttattaatct ccaagttatt ctaccattta aataaagtta
    28141 ataaaagtaa ttgtttataa tctttataat taagttatgt atacatatat tcctatgcgc
    28201 tatcttaatt aatgatttta aaatccaagt ttgcaatttg aacatccagt ttcccaaaat
    28261 ctattgtcaa aactcacttt agttattaat ataatgatat aaatatataa ctgcatagat
    28321 gttacacact taactattta atatgatacg atttggtatc taaatttata tattgtatat
    28381 agggggagat atatatgtat aaagataaaa gtgatgaatg tatacactta atgactgcat
    28441 acatcgatag tataagtgga tattattcat ttattgacac acaattagag gattttatga
    28501 tgaaatatgg tgaaaacatt gttgattcaa atttacacag tataatgatg ttgctttgta
    28561 aatggggatt atcttagcat ttcaagaaac ttttttgtat attgtatcct atattattta
    28621 ttcattaaca aaaaagattg acataaactt aaaagctata tatagatttc tttaaaaatc
    28681 tatatatagc ttttttaatt ttttcaaaat gtatacttaa ataagttagt ttttttcaaa
    28741 atttgactgt ctaatatgat ataatttaac tatctacaac aataaaaaat atctaatatg
    28801 tattatctag tttagctcat cataataaca tccatatatt tacaattaag atattttcaa
    28861 tatagtaata ctcaatttta atattataaa aaggagagat tttgtatgaa ttacaatgac
    28921 ttactaaaat cagctagaga gaactttaat ggaacatgta aggtatgtaa aatttgtaat
    28981 ggattagctt gtgctggaga tgttcctggt atgggtggta aaggaagtgg ttcgtctttc
    29041 attgaaaata gaaaaagtct agaaaaaata aagataaata tgagagtcat acataatgtt
    29101 tcaaagccag atacatccat agaattattt ggtagaaaaa tgagttctcc tatattcgct
    29161 gcacctgtat caggtactat attgaacatg ggtggtaaag tttccgaaaa agaatacata
    29221 gagcctgttg ttagaggttg ttctaattct ggaatttatg ctatggttgg cgatactaat
    29281 gtagatactt ttttactgga taatttagat gtattgaagg ataatagggg aaatggaatt
    29341 gtatttataa agccttggaa taattcaaag ataatagaaa aaattagatt gtctgaagaa
    29401 gcaggtgctt ttgcagttgg tgtagacctt gatgcttgtg gacttattaa taatcaattt
    29461 caagaaaatc ctttttctcc taaaacaatt gatgagatta gagaattggt tgaatccact
    29521 agattacctt ttattataaa aggaattatg actgtagatg atgctttgat ggctgtagag
    29581 tctggtgcta gtgccattat tgtttcaaat catggtggaa gagtacttga ttatactcct
    29641 ggtacttgtg aggtacttcc agatatagct aagtctgtaa agggaaagat aacaatactt
    29701 gttgatggtg gagttaggac tggtgttgat gtagttaaga tgcttggatt gggtgctgat
    29761 gctgttttga tgggaagacc ttttgttact gcttcttttg gcggtggtct tgatggtgtg
    29821 gagtttttta tagagaagat taggaatgag ttatgtgaga ctatgatttt gactggttgt
    29881 caaaatgtta aggatataga tggtagagtt atatggaatt tattgtaaaa tatttctaga
    29941 ctctaagata ctgattaata tattctatat aaaaaggttg tcgccctatt tcttagtgcg
    30001 acaacctctt ttctaataac gctctccggc agcttgccaa gtggcttttc caacaacacc
    30061 atcttttgtt acaccaagtc ttccttgaag tatttttact tgattatatg tagcaggtcc
    30121 aaatttacta tctggatcaa tatcagaacc atttatttgt tgacataaaa gttgtactaa
    30181 atgcacagga ttcccttttt ctacatgtcc atatcctact acagtggtat ttatatttac
    30241 gctataactt ataggaccag taacatcaaa acgatttaaa tcttttttat atctaataat
    30301 aaatgctttt ggttcaatat tataatcatc tatattaata ttatatgaat ctataaattt
    30361 ttcattaata aagattgtat catctttaga tggatttata tttaattctt tacatttttt
    30421 ttcataatca gattttatag gttgtggtgc attctcataa ataaattcat aaccagttat
    30481 tgtaaatgaa tttgttgttt gctcttcatt actttttgat acatttacat cattattagt
    30541 acttgcaaat actaaactag tagatattag agtcaaacac cctgtgacta ttaaaatttt
    30601 ctttagactt ttcatttcaa aattcctcct aatttgttaa taaattataa tactataaaa
    30661 atctattaat ttacaactct catgctaata tcatcttctt ccttaagatc tgcgcctgtt
    30721 ttttctaaaa tccctatatt tcttaaataa tttgaattat caaaactaat actttcaaaa
    30781 ttaaaatatg taactgaaaa taatgtaaac aaaatcaata acatcaatat tccacttttc
    30841 ccttttttct taataatact atcaagtctc ttttccaaat ttgtcttatt aaattcagtt
    30901 gttaaaatac cactattata attcttacta agtttaattg actttataag tgctaacgca
    30961 tatgctttct tatcttttaa agagcaatca gtcaaaacac tttcatcaca acataattca
    31021 caatctagat ttactttatt gctcattata taaacaagtg gattaaacca atacattatt
    31081 ttaacagaca aaactaaaaa ctttatcaaa atatctttgt ttttaaaatg cattaattca
    31141 tgtttcaaaa tccaagttaa ctcatcttta ctataagtat agtctggtaa gaatatgtac
    31201 gatttaaata atcccatacc tgctggactt atcaattcat cagaacccct taactcaatt
    31261 ttctttttta tgtctaattc ttttaacaaa tttttataga tacaatttac atcattatct
    31321 tcaatatcat aagatacatc aacaacgaga tttttaaatt ttgtgtattc tataaatgta
    31381 taaactgcaa tggctatagt tacaaataac cataaataaa ctaaatacag cataaaatca
    31441 ttatctatag aaatatcaat ttgtgtaata ttatttccta ctgtattttc tttgggatct
    31501 tttacttcat acattactgt ataattaaat agaaaaagta acattctaaa gactattacc
    31561 aaccaaatat aataattaaa tctcttacta aacctcttaa aaaaatatat tttcaaaaat
    31621 aataatagac aaataccaaa actacataca atagttgtct ttataagatt gtcaaaaaca
    31681 tatagcatat aattattcat tctttaaatt atcaaaataa ctttctaaaa aatctagcgt
    31741 gtctttacct atattttcat catcatgcaa tgctgaaatt aagcttttca aagaatcatc
    31801 atgtatatta cttaggaaat cctttgtctc aaaacttaaa taatctttgt gttttactat
    31861 tactgtgtaa tgtgtatatc tgtctatttt ttgagcatct aagaatcctc tttttactaa
    31921 cctagataaa agtgttagtg tagtggtttg tttccaacca tatttttgtt ccataacttc
    31981 aataacatct tttgatgtca ctgtagcatc tactttccaa ataaatttca ttacttttag
    32041 ttctgcttgt ggtatttttc ttatcatcat ttacacctcc ctcaaaactc ttgctacttt
    32101 aagtctacaa tatgtaaatt ttttagtcaa acgctttatg aaaaaatgtg aatttttgta
    32161 ctttttatta attttctaat ttttttaaat attaaaagtt agattttaaa atatagttat
    32221 tcaagtattt tgctttttac gtatttaatt ataatagtgt agaatgttat ttccacatta
    32281 aaattattta acaaaactac ctatattttt acacaaggaa tattataata cttttattta
    32341 aataaactag agtaatatct aataaatttt aacaatctcc taattgtacc tttgtgttat
    32401 taaaatatct gttttatata agtttatgtt ataagtaaaa ttatacatat caaataaagg
    32461 tataaaaaca ccattttgag tgtctttaaa ataaagtttg tattcttcta aaaagttata
    32521 ataattaaaa catcactact atacaaagga agatgtacac tttttatgtt taataaaaac
    32581 taaaatatta aaaaaattta ttgaagaata tgatgtcaaa acaattacag atattcaaga
    32641 tatactaaaa gatttatttt aaagtacaat ccattaaatg ttagaagcgg agcttgatga
    32701 acttttaggt tatgatagaa cgataataag aacaaaaata ctaaaaactc tagaaatgga
    32761 tatagaaata aaaatattaa atcagatttt ggagaaataa ctttaaatat ttcaagaaag
    32821 cgaaatgaag attttgaact aaaggtaatt agaaactatg aaaatgatat attaggaata
    32881 aaaaatcaag tgattggaat atacttaaaa ggcatgtcta ctcaaaatat attaagtcat
    32941 cttaaaagta cttatggaat tgatattatc tcattcacta ataactaaaa ctactgataa
    33001 agttattcct ttagctcata aatggtaact tagacctctt aatataattt attcaattgt
    33061 atttatagat gctatacatt ataaagttag aagtgaggtg gaataataaa caaaacagta
    33121 tatatagcta tatgtattaa cttagaaggc ataaaagggg ttttaataat atgaaatagt
    33181 acaatcaata tacaatagta ttaagaggtt ggtgaaacaa ttggaatgaa ttagtaacat
    33241 attttaagta tcctcaagaa ataaatacat cgatatatac tacaaattca atgtaaagtt
    33301 agaatagatt attaagaaaa gttactaaat tgaaatctat ttttactttg gataattatt
    33361 tgcacacatc actatactta acaaccatgt atatttctta aaaatggact cagaaaatta
    33421 gaaattgtgc tcaaatttta gctcatttaa gtatttattt tgaagaaaga aatttttcaa
    33481 aataaacaag aatttttctt aaagggttaa tagtttttgc aaaaatgaaa aatatatcaa
    33541 aataaggcta tgatttttaa agccatagcc ctattagtaa aaatttaatt atcacttaaa
    33601 aaataaattt ttgtttaaag aatacatgta aatgtttaca gattcagcaa tttaaaatta
    33661 tctgtgcaat agctttcttt ttaaagacgt cttccagctg catcccaagt acttcttcca
    33721 acaattccat ctcttgttaa tccaagtctt ccttgaagtg acttaactgc attatatgta
    33781 gcaggtccaa attcactatc atcatcaata cttgaaccat ctaaggttct acataaaagt
    33841 tgaactaaat gcacaggatt acctgattca acacgattat atccgactac agtattactt
    33901 acatttaatg tataactctt aggtccagta acttcaaaaa cataaccttt tttatatcta
    33961 ataatatatg cttttggctc aacatagttt tctacatcat ttgcatctat agaatctttg
    34021 ttaacaaaaa ttatatcatt tttagatggt tttatattta gcgcttcaca ttttttttca
    34081 taatcaaatt ttacagattg tggtgcgttt tcatatgtaa attcataacc agttatagta
    34141 catgaactta ttattgaatc atcatccttt gatgtatcta catcagtagt agtatttgca
    34201 aataccaaac tagtagatat tagagttaga caacctgtaa ttattaaaat tttttttaaa
    34261 tttttcattt cataattcct cctaatttat taataatatg acaatatacc ttttcattac
    34321 atatattaat ctattaattt ataactctca tgctaatatc atcttcttcc ttaagatcta
    34381 ctcctgattt ttgtaaaatc cccatattcc ttaaagaatt tgaattatca aaactaatac
    34441 tttcaaaatt aaaatatgta actgaaaata atgtaatcaa aatcaataac accaatattc
    34501 cacttttacc ttttttctta acaatactgt caagtctctt ttccaaactt gtcttattaa
    34561 actctgttgt taaaatacca ctattataat tcttactaag tttaattgac tttataagtg
    34621 ctaatgcata ctccttctta ttctttaaag aacaatcaat caaaacactt tcatcacagc
    34681 ataattcaca atctagattt actttattgc tcattacata tacaagtgga ttaaaccaat
    34741 atattattct aacagacaaa actaaaaact ttattaaaat atctttattt ttaaaatgca
    34801 ttaattcatg tttcaaaatc caagttagtt catctttact ataaggatag tctggtaaga
    34861 atatgtaaga tttaaataat cccatgcctg ctggacttac caattcatca gaacctctta
    34921 attcaatttt cttttttata tttaattctt ttaacaagtt tttgtagata cagtttatat
    34981 cattatcttc aatatcataa gatacatcaa caactagatt tttaaatctt gtatacttta
    35041 taaatgtata aactgcaatg actatagtta caaataacca taaataagct acatacagca
    35101 taagattatt atctgtagaa atatcaattt gtgtaatatt atttcttact gcattttctt
    35161 tgggttcttt tacttcatat actattgtat aattaaatag aaaaagtaac attctaaaga
    35221 ctattatcaa ccaaatataa taattgaatc tcttactaaa cttcttaaat aaatatcttt
    35281 tcaaaaataa taatagacaa ataccaagac tacatacaat agttgtcttt ataagatttt
    35341 caaaaacata tagcatataa ttattcatcc tttaaattat caaaataact ttctagaaaa
    35401 tctagcttat ctttacctat attttcatca tcatgtaatg ctgaaattaa gcttcttaaa
    35461 gagtcatcat gtatattact taggaaatcc tttgtctcaa aacttaaata atctttgtgt
    35521 tttactctta ttgtgtaatg tgtatatctg tctatttttt gagcatctaa gaatcctctt
    35581 tttaccagcc tagataaaag tgtcagtgtg gtggtttgtt tccagccata tttttgttcc
    35641 atagcttcaa taacatcttt tgatgtcact gtaacatcta ctttccaaat gaatttcatt
    35701 acttttagtt ctgcttgtgg tatttttctt atcattattt acacctccct caaaactcct
    35761 gctaccttaa ttctacaaca tgtgaatttt ttagtcaaac actttatgaa aaaaatgtga
    35821 atttttgtac tttttgttaa atttctaatt tttttacaat attaaaagtt agattttaaa
    35881 atatatctat ttaaatattt tactttttac atatttaatt acaatagtgt agaatgttat
    35941 ttacacatta aaactgttta ataaaaattc ctatattttc acacaaggaa tattataat
//
LOCUS       DAENCV010000035        33593 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAENCV010000035 DAENCV010000000
VERSION     DAENCV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.035"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     regulatory      88..178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            588..905
                     /gene="flgB"
                     /locus_tag="KQD82_001640"
     CDS             588..905
                     /gene="flgB"
                     /locus_tag="KQD82_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG4380114.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            913..1317
                     /gene="flgC"
                     /locus_tag="KQD82_001641"
     CDS             913..1317
                     /gene="flgC"
                     /locus_tag="KQD82_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG4380115.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1327..1641
                     /gene="fliE"
                     /locus_tag="KQD82_001642"
     CDS             1327..1641
                     /gene="fliE"
                     /locus_tag="KQD82_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG4380116.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            1666..3216
                     /gene="fliF"
                     /locus_tag="KQD82_001643"
     CDS             1666..3216
                     /gene="fliF"
                     /locus_tag="KQD82_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG4380117.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3229..4299
                     /gene="fliG"
                     /locus_tag="KQD82_001644"
     CDS             3229..4299
                     /gene="fliG"
                     /locus_tag="KQD82_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG4380118.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4292..5023
                     /locus_tag="KQD82_001645"
     CDS             4292..5023
                     /locus_tag="KQD82_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG4380119.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYNEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5027..6346
                     /gene="fliI"
                     /locus_tag="KQD82_001646"
     CDS             5027..6346
                     /gene="fliI"
                     /locus_tag="KQD82_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG4380120.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVYATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRGIFE"
     gene            6378..6824
                     /gene="fliJ"
                     /locus_tag="KQD82_001647"
     CDS             6378..6824
                     /gene="fliJ"
                     /locus_tag="KQD82_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG4380121.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSIGKNNDSVQNQKITINYLLALNNSIMDLSEKLDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6852..8057
                     /locus_tag="KQD82_001648"
     CDS             6852..8057
                     /locus_tag="KQD82_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG4380122.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNDKFEDNLYKTTDHKKV
                     KDRRETSKQNVSSDRPENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8075..8683
                     /locus_tag="KQD82_001649"
     CDS             8075..8683
                     /locus_tag="KQD82_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG4380123.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8710..9693
                     /locus_tag="KQD82_001650"
     CDS             8710..9693
                     /locus_tag="KQD82_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG4380124.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9706..9909
                     /locus_tag="KQD82_001651"
     CDS             9706..9909
                     /locus_tag="KQD82_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG4380125.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9909..10727
                     /locus_tag="KQD82_001652"
     CDS             9909..10727
                     /locus_tag="KQD82_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG4380126.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10717..11412
                     /locus_tag="KQD82_001653"
     CDS             10717..11412
                     /locus_tag="KQD82_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG4380127.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11424..11867
                     /locus_tag="KQD82_001654"
     CDS             11424..11867
                     /locus_tag="KQD82_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG4380128.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11882..12256
                     /locus_tag="KQD82_001655"
     CDS             11882..12256
                     /locus_tag="KQD82_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG4380129.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEVLKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12257..12922
                     /gene="fliP"
                     /locus_tag="KQD82_001656"
     CDS             12257..12922
                     /gene="fliP"
                     /locus_tag="KQD82_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG4380130.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12935..13204
                     /gene="fliQ"
                     /locus_tag="KQD82_001657"
     CDS             12935..13204
                     /gene="fliQ"
                     /locus_tag="KQD82_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG4380131.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13216..15030
                     /locus_tag="KQD82_001658"
     CDS             13216..15030
                     /locus_tag="KQD82_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG4380132.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYSVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFNMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15039..17114
                     /gene="flhA"
                     /locus_tag="KQD82_001659"
     CDS             15039..17114
                     /gene="flhA"
                     /locus_tag="KQD82_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG4380133.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17089..18105
                     /gene="flhF"
                     /locus_tag="KQD82_001660"
     CDS             17089..18105
                     /gene="flhF"
                     /locus_tag="KQD82_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG4380134.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDAM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18102..18980
                     /locus_tag="KQD82_001661"
     CDS             18102..18980
                     /locus_tag="KQD82_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG4380135.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNVISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18994..19695
                     /locus_tag="KQD82_001662"
     CDS             18994..19695
                     /locus_tag="KQD82_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG4380136.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19711..20133
                     /locus_tag="KQD82_001663"
     CDS             19711..20133
                     /locus_tag="KQD82_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG4380137.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            20118..20303
                     /locus_tag="KQD82_001664"
     CDS             20118..20303
                     /locus_tag="KQD82_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG4380138.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20344..21117
                     /gene="flgG"
                     /locus_tag="KQD82_001665"
     CDS             20344..21117
                     /gene="flgG"
                     /locus_tag="KQD82_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG4380139.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21138..21899
                     /gene="flgG"
                     /locus_tag="KQD82_001666"
     CDS             21138..21899
                     /gene="flgG"
                     /locus_tag="KQD82_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG4380140.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21922..22803
                     /locus_tag="KQD82_001667"
     CDS             21922..22803
                     /locus_tag="KQD82_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG4380141.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22806..23180
                     /locus_tag="KQD82_001668"
     CDS             22806..23180
                     /locus_tag="KQD82_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG4380142.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23212..24615
                     /locus_tag="KQD82_001669"
     CDS             23212..24615
                     /locus_tag="KQD82_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG4380143.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24731..26668
                     /gene="htpG"
                     /locus_tag="KQD82_001670"
     CDS             24731..26668
                     /gene="htpG"
                     /locus_tag="KQD82_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG4380144.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKHYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLFIEL"
     gene            27056..28186
                     /locus_tag="KQD82_001671"
     CDS             27056..28186
                     /locus_tag="KQD82_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG4380145.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNSGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28413..29435
                     /gene="splB"
                     /locus_tag="KQD82_001672"
     CDS             28413..29435
                     /gene="splB"
                     /locus_tag="KQD82_001672"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG4380146.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29617..30528
                     /locus_tag="KQD82_001673"
     CDS             29617..30528
                     /locus_tag="KQD82_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4380147.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30543..30959
                     /locus_tag="KQD82_001674"
     CDS             30543..30959
                     /locus_tag="KQD82_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG4380148.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31059..31451)
                     /locus_tag="KQD82_001675"
     CDS             complement(31059..31451)
                     /locus_tag="KQD82_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380149.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31592..31987
                     /locus_tag="KQD82_001676"
     CDS             31592..31987
                     /locus_tag="KQD82_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG4380150.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            32390..32596
                     /locus_tag="KQD82_001677"
     CDS             32390..32596
                     /locus_tag="KQD82_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380151.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(32753..33484)
                     /locus_tag="KQD82_001678"
     CDS             complement(32753..33484)
                     /locus_tag="KQD82_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380152.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
BASE COUNT        13707 a         3579 c         6065 g        10242 t
ORIGIN      
        1 ttggtaacta aatataaaat ataaaatatt tgattataaa caaaaaagat atttaaaaaa
       61 tttgttattt attgtatact taagttaaaa ctaaataggc aaatctagag aaatctagtg
      121 acgcaaagct atagggacta aggtttatat acatgaacta tgtcagccag ttgccaaaaa
      181 gagtcctagg tgtattgtat acctaagaaa agtctagaat gactgccttt ttggcagtca
      241 ttttgttatg aaaaaatcac tttatttcag tagaaaatac ctataattaa catactttac
      301 caaattataa cataaaataa agaaaaatta tcttaaaaag ttactatttt acaaaaaaga
      361 gcaacttttc gaagaaatat ttaaatacat attaaaattt ttttatattt tcatgggact
      421 tcattgaaaa aatattagtt ttcttactaa aatgatacat tattgtatta atgtaatgga
      481 aatgcgatta atttaaatga ataaagaagt cattttttgt aatatagcaa cttcattttt
      541 ttattaataa gcaaaataaa taaaatatat tttgaggagg agtgagtgtg aatacatatg
      601 acattataaa aatgggatta gatgcaacta atttaagaag tcagacaata gcaaataata
      661 tagcaaatat aaatactgaa ggatataaga gacattatgt aacttttgaa gaaacattaa
      721 atgatgctat gcccaaaata gaagaaaaaa cagataattc taaaggatta agagtggatg
      781 gcaataatgt agatatagaa gaagaaaaag taaaccaagc aatgacaaca ttacaatata
      841 atgctttgat aagttttgca aatggaaaaa tagcaatgac taaatcaata ataagtggga
      901 ggtaattaaa aggtgagtgt gttcagtgga atgagaatat ctgctagtgg attaacagca
      961 gaaagcttga aaatggatat catatcgtcc aatgtagcta atataaatgc cacaagaggt
     1021 ccaaatggag aagcatatag gagaaaagtt gtttcatttg aagaaaacta tgataataaa
     1081 ttaggtatgc taggagtaaa agttacatct gttgaagatg ataaatcacc tcttaaaaaa
     1141 gtatatgacc catctcatcc agatgcaaag ccaaatggat atgtagaata tccaaatgta
     1201 aatatattgg tagaaatgac agatttaata acagcttcac gagcatatga ttctaacgta
     1261 gatacattga atgctcagaa gtctatgata tcaaaagctt tggaaatagg aaagtaggga
     1321 taaattatgc caattacaca aattaacaat ttaatgcaag gtgcaaagtt agataatttg
     1381 attggagaaa caaaaccatt aactcttaat aaagaagaaa aaaagaattt tgaagatgta
     1441 ataatagata ctatagataa agttaatttt caacaagtga cagcagatga tatgaaagaa
     1501 aaatttataa aaggcgaaga tgtttctatg catgaagtta tgttaatagg tcaagaagca
     1561 cagctatcta tgcaatattt aatagaagtt agaaataaga ttcatgatgc atatcaagaa
     1621 atgaataagg tacaaattta gccttttgaa caggagtaat aatcaatgat agccagtttt
     1681 aaaaataaga ttaaagagca agttgctaaa tctaggacaa agataagttc tctaaaaaag
     1741 ggaactaaaa tagcattagg tataggggtt attgctatat gtttagcgct agtatttaca
     1801 gcaatatctg cgagcaaaaa taagtatgca gtcttatttt cagaactaga tgttcaagat
     1861 gctgcagata tatcagcaga attagagtct aaaaagatag atactaaggt aaagggaagt
     1921 acgatttatg tacctagaac agaagttgat aagttaaggc tggagttagc accaagtctt
     1981 acaagtggtt ctaaaggatt tgcactaatg gatgatagtt catcaatagg acttactgat
     2041 gaagagtttc agattaagaa gcaaagaatg atacaaggtg aaatagaaaa aacaataaag
     2101 agttttccac aagtggaaga cactagagta catataacat ttggagagtc atcagtattt
     2161 gagaaggagg ctgttccagg gagtgcagca gtttatttga tgctaaaacc agggacgact
     2221 ttagaaaaaa accaaataaa gtctgttatg gcacttatat caggaagcgt tacaaatata
     2281 ccagagaaaa atgttcaggt tattgataac aaaatgaatt tattatctga aggtattttg
     2341 gaggatgaaa atgttaatga aaataatcaa ccaataaata gtggcattag tgttgataaa
     2401 aataaggcat ctgaacaagc attgaataaa caactagaaa agtctatttt agaaatacta
     2461 gaaccaatat ttggtaaggg aaaggtgaaa gctacagtaa atgctgattt aagttttgat
     2521 acagctgaaa aaactgagat aaaaattgac cctgataaag ttgcaataaa agagacaagg
     2581 tcgaaaaatt catctaaatc tgctacaaat aatgttcaag gtgtagatgc taacatgaat
     2641 aaccaaggaa ataacaatgg tactaataca gaagatagtt tagatgagag ttttgagtat
     2701 gaaacaggta agattgaaac tcatactata gttgcgcctg gagaacttaa tagattgaca
     2761 gcttctgttg caatagatgg aaaaatagct aaaggtgttc aagctgatgt agaggatatt
     2821 gttaataatg ctataggtat ggatggagcg aggggagata ctcttagcgt tgtttcaatg
     2881 gtatttgacc cagaaggtaa gaaagaggct aaacaagagc tggaagagat taaaaaagaa
     2941 ggatttaaaa agaacataat aaaagcagtt attggagtag tggcagtagt agcaattgga
     3001 gctattggat acttgatata taagaaaaga agtgaacgag aagatatgga ttatgaagat
     3061 gaagattata tttctaaatc tgattctgtt gatagaaaag ataatcctct attaggagat
     3121 gacccagaac tgacattaga ggaagctgtt aaaatgtatg cagaagaaaa gccagaccaa
     3181 gtaacagaga taataaaaac ttggttaaat gagtaagggg gatagaaagt gtccacggaa
     3241 attacaaatg ttaatgcgtt tgacttaagt gatataaatg agagtgataa gaaagagaag
     3301 atagaaggtg agggtttaaa aaaagctgct gtattattaa tgacattagg accaaatgtt
     3361 tctagtgata taataaaaag cttgcctgat aaacaagttc aaaaaatagg tttggaaatt
     3421 gcaaatattt cttcaatagc ttcaaaagaa agaagagaaa tcttaaaaga gtttgtagaa
     3481 ataaataggg caaaagatta tgttatagaa ggtgggcttg aatatgcaag agagttgtta
     3541 agcaatgctt taggaaatgc caaagcaaac aaattacttg aaggtatatc aataaataca
     3601 agcacaaagc cttttaaaca tattataaaa ctagatgtaa agaaaatatt agaaatactt
     3661 aggaatgaaa gtgcacaaac tatagccgtt ttacttgcac atatggagcc agaaaatgga
     3721 gcagaagttt tatcatactt agattttgat ttacaaaatg aagtagctat gaggataagc
     3781 tcaatgggtt caatatctcc agaagtaata aaagtaattg aaagttcatt agaagataaa
     3841 ttacactcaa tgagtggtga tgattttaag gattatggag gagtgtctac cttagttcaa
     3901 atattgatta attctgatag aaaaacagaa aagaatattt tgggaactat tgaagaacaa
     3961 gacccggatt tagcagctca aattaaatct catatatttg tctttgaaga tattgtcaag
     4021 ttagatgata taagtataca aaaagtgtta aaagaagtga ctatgaaaga tttagtatat
     4081 gccttaaaag gtgtggatga tgagatttca gatgctatat atagaaatca atcttctagg
     4141 gcagcagagg cacttagaga agaaatggaa atggttggtg tcataaaagt tacacaggtt
     4201 gaggctgctc aacaaaagat agcaaatata gtaagaaaac ttgaaagaga aggtgccata
     4261 actattgtaa ggaattttgg tggtgacttt gttgaataat aagattatta aaagtaaaaa
     4321 agcattatat aaaggagtgc tagatttaac cataatagat aaagttagag tgggagaatc
     4381 aaatgtaaat aaagaaaatg attgtagatt tagggatgag gtaataaaag aagctataga
     4441 atctgctgag ataaaaaaga atgagatatt agaagaagca aaaatgaatg cacaaaatat
     4501 agaaaagcaa gcatatgaaa aagggtatag tcaaggtcaa aaaaatggat atgaagatgg
     4561 atataaagag tcatataatg aatataccca aaaagctaaa gatgaagcag attttataaa
     4621 aaatcaggca gacttaatac tattacaagc taatgagaag atgactgagt acataaaaga
     4681 aaaaaataaa gagattgtaa gacttgctat taatatggct agttctgttc taaaaaagca
     4741 tttcgaagat gaagaatcta tgagtgattt gttaaagcaa attattcaag attatgaagg
     4801 aagtagttca tttatagtaa gatgtaattc tttctataaa gataatatag agaaagagtt
     4861 tgaattattc aaagaagaaa catcactaaa atcaagtgta tttgttattg cagatgatag
     4921 tatagataaa ggcaatgcta tcatccaaaa ggaaaatgga agaataatag ttggtatcga
     4981 ttgtgctatg gaaaaactaa aagaagaatt gcttggtgaa taaagtatgt ataaaactga
     5041 tatatctata gattttgaca aaataaataa gaaaatagaa aatttagaac tcactatatc
     5101 agaaggtaga gtcaaaaaga taataggact tactgtagaa gttgaaggaa taaaagcttt
     5161 tgttggagag ttatgtgtta tatacaatca agtcaataaa ccagtgaatt gtgaagtagt
     5221 gggttttaaa gataaagaag tcatattaat ggcgttggga gagttaacat tgatagctcc
     5281 tggttgtaaa gttatttcta aggggatacc tttaagtgtt atgtgtagcg ataatttact
     5341 aggcaaagta cttgatggtc ttggaaatcc tatagataac tcagatgctg ttttaggaga
     5401 tagatacaac ttgaacaatg aacctcctga tccaatgaag agaaaaaaga tacgaaatat
     5461 aatggaaaca ggtgtaagag caatagatgc atttactact tgtggtgaag gacagagaat
     5521 agggatattt gcaggaagtg gagtaggtaa aagtacgact ctaggtatga ttgctaggaa
     5581 tgcaaaagca gatgtaaatg taattgccct tattggagaa agaggaagag aagtattaga
     5641 ttttattgat aaagatttag gtgaagaggg aatgaaaaaa tctgttgttg tatatgcaac
     5701 atcagataaa gctcctttgg ttaggctaaa aggtgctctt actgctactg caatagcaga
     5761 gtattttaga gaccagggca aaaaagtaat acttatgatg gactcggtaa ctagatttgc
     5821 tatggcacaa agagaagttg gtgtagcaat aggagagcca ccagctcaaa aaggatatac
     5881 accatctgtg tttgctattt taccaaaact tatggagaga acagggacat cagataaagg
     5941 ttctataact gcattttata cagtattagt tgatggagat gactttaatg agccaatagc
     6001 agatacaaca agagggatac ttgatggtca tatagtttta tctagagact tggcaaataa
     6061 gaatcattat ccttctatag atgtattgaa cagtttaagc agacttatga atgagattgc
     6121 ttcaaaagaa gacataaaaa tagcatcttt tgcgagagat atgcttgctg agtatagaga
     6181 agcagaagat ttgataaata taggtgcata tgcttcaggt acaaacaaaa aaatagatga
     6241 agcaatctac tatcatgagc atattattaa ttttttaaaa caaggaataa atgaaaagag
     6301 ctcttttaat gaaactataa gtagtttaag aggaatcttt gaataaacaa tttaaaaaaa
     6361 tatttgaata ggttgtgatg gaattggata aaaattttaa gtttagatta cagaaagttt
     6421 tagacttaaa aataaaagat gaagaagaga taaaaatgga atttgctaag attcaacaaa
     6481 aaaaaataga tatagaaact aatttagaaa acttagaaag taattacagc aaatattcta
     6541 taggtaaaaa taatgatagt gttcaaaatc aaaaaataac tattaactat ttgctagctt
     6601 taaacaactc tataatggat ttatcagaaa aactggataa gtcgacaaat gaacttgaaa
     6661 aagctagaaa acagcttata agcaaacaga tagagagaaa gtcattagaa aaattaaaag
     6721 aaaaaaaata tggacagtat tataaagaag aacagttaaa agaacaaagc actaatgatg
     6781 agtttgcaag catgagttat ttgagaaata gacaagttct ataaaatagt aggaagtgag
     6841 gatattattg tatgaagata caagcagatt cttataatat tattaaagat attgggaata
     6901 gaaaaaactc atctaagaat aaaaatataa aaaatgataa atttgaagat aatctatata
     6961 agacaacaga ccataaaaag gttaaagata gaagagaaac aagtaagcaa aatgttagct
     7021 cagatagacc ggaaaatagt gataatgagt gtcatttaga tgaagataaa agtaatcact
     7081 tagaagatga aaatacatat gaaaaagatt gtagtaataa taatgaagct ttgcagcaat
     7141 tactgaatct tttaaaagag aattataaaa aagataaaga tataaacact atattgaata
     7201 atataaaaaa actaagtata tcagatgaat taaaacaaga acttaagctt aataaaaata
     7261 tattaaataa gattgcaaat caaaattcag agctaaataa agctaatata aataactcta
     7321 atgatataga aaatttagac atagttaaat taagagaact tttggaggat aatgctaata
     7381 aagatattga agtaattact aaaactacag aaaatgatac taaaaatata gatttgtata
     7441 gttttgatag caatagaatg gataatctta actcaaataa acagaaagat gatagctctg
     7501 atattttgga aaaactagca ggggtaaatg gaaataagag tgggaatttt ggacaagtaa
     7561 taaataaaaa tgttgatatg aataaaatta aaaatgataa tgtcaattta aaacagatag
     7621 aatcaaatgt aatggatgac tctataaaag ctattaagca tatgaaaaca aatgatatac
     7681 aagaattgac aattaaatta agaccaaaag aattaggaga tatgaatata caattgttaa
     7741 aagatggtga aagtatgaga gctgttgtga ctgttttcaa taaagatgtc tttgactcta
     7801 taaataaaaa catagcagat ttaaagcaac atcttgaact tactaatgta aatataaaag
     7861 atgtatcagt gcaaatgcac agcgataata caaatacatc tgataccttt gataaagcat
     7921 ttgaacaaca gaatagacaa aataaccaag aaaacatgag taagaagaat acaaatgata
     7981 agcataataa tgtagtcata gaagatgaca ttaaagaaaa tactattgat gatgatagag
     8041 tagatttact agcttagtaa gggggaagta ttgcatgtac ggaatagcaa cggctttggc
     8101 aggaactcag ggaaactata ggtcaaataa cttaagccaa cttgaaagaa atattaatga
     8161 tattgccaat aaacaagtta gaagcaaagg aaatcagaca gtaacagaca atgggactcc
     8221 aattatagga caaggtgatg atgctgataa agacttgttt atgaagatac ttgtggcaca
     8281 gatgggtaat caagacccaa tgaatccgca agacccaaca gaatatgtgt cacaattagc
     8341 acagtttgca actttagaaa agatgtcagc tatgaatgag aagctagatg tacttttagc
     8401 cttaagtaat tccactctaa taaactctgc tttaagtatg gcaacatcat taattggtaa
     8461 agaagtagaa gtatctgggg aagagaaaga agatgaaaac attgtaggag tagtaaagtc
     8521 cacatatata aaagatgggg aagtacactt agaattagaa gtagatggaa gtgacaaaac
     8581 tgtaggtgtt aaatatgaaa aattacttaa aataggagag gtagatgaaa attcaggaga
     8641 agaaaatgat tcttcagaac acgaatctcc agaagaagca taatattaaa tgagaaaaga
     8701 ggtaaattca tgataaaagc aatgtattca ggtgtaagtg gaatgaaagc aaatcaaaca
     8761 aaattagatg taataggtaa caatgtagcc aacgctggaa ctacttcttt taagaaaagt
     8821 tcagctagaa taacagattc attttatcaa actgtactat atgcaagtgc tccaacagct
     8881 gcattaggag gaacaaactt aggacaagta ggtgtaggta gtaagatatc aagtataaat
     8941 aaagatatgg tacaaggaaa tgtacaacca actaatagac catctgattt aatgatagat
     9001 ggagatggat atcttccagt agtaagacaa ggaacagtta tgtatacgag agatggttct
     9061 tttaatttag atacaggatt taaagcagct gatggtgctt tgaagaatat taaaggtgga
     9121 agacttgtaa atggtgatgg atatatgtta caaggtatga tttatgatgg ggaatataaa
     9181 gctgatactg gagaatttgt taaaaagggt gatgctaaag gaaaagagcc tattgtaata
     9241 ccacttcaaa gtataagtcc taaggatgga acagttcaaa atgtactaga gtataatata
     9301 tcaaaggatg gaacagtaga atttttattg agtgatggtc aaagaactca atttgtctat
     9361 gatatacctg atggtgctaa ggtaggagat acacctgctg gaatagataa attaaaaggt
     9421 acaatacaaa aagtgcaaac atatgcattc caaaatccag caggactaga tgcagaagga
     9481 ggaaacttgt ttaaaccatc agcaaactca ggagacccac aaatagcagg agctacaggc
     9541 acaataatac aaggggcaat agaagcttcc aatgtagata tagcagaaga atttacagaa
     9601 atgataatag caagtaggtc tttccaagca aacagtaaaa taatatcgac atcagacgag
     9661 atacttcaag aaataataaa tcttaagaga tagtaggtac atattatgat aagagtaacg
     9721 gatttaaacg gaagaaaata catattaaat tcagacctta tagtgaggat tgattctgta
     9781 ccagaaagta ggattctcct tacaacagga gaaaaaaacc ttgtaaaaga gtcggtagat
     9841 gagatagtag ataaagtaat aaagtataaa agaaaaataa atgtaggtta tattaggagc
     9901 gaaaaataat gaaaaaaaca gattcactga catttattgg actaatagta tctacagtat
     9961 tagttttagt tggtgccgca aaaggaagta gttcagggtt aaagaatttt tttgatgttt
    10021 catcaatatt aatcacagtg cttggttcat ttggagctct aatgattaca tttactatag
    10081 atgatataaa acttataaaa aatgcattac aatactcatt taagacaatg agtgtatcaa
    10141 agttagattt actagaacaa tttaaaacac tttctaaaaa agctagaaaa gaaggtcttt
    10201 tatcaataga gagtgatgta atgggaatag aagatccatt tatgaagaaa gggttagagc
    10261 tgtgtattga tggattggaa atcaatgaga taaggtccat cttagaacta gagatagatt
    10321 ctgttgaaga tacaaataac agatactcta aactatttaa aacttgggga acctttgcac
    10381 ctgcttttgg gatgttggga acactaatag gtttaataca aatgttagct gaccttacat
    10441 ctccagattt aatagcatct ggcatgggaa aggcattaat tacaacgttt tatggttctt
    10501 tacttgccaa tatcgctttg aacccaattg catataatat agatgagaag actgaaaaag
    10561 aaatttatgt aaaggaaatg atgttggaag gtataataag catacaatct ggggagagca
    10621 gtatagtagt tgaggaacgt ttagccacat acctttcaaa caatgagaag ttagaaatta
    10681 tgaaatccaa taaaaataca gaaagagcaa tgagcaatgg cgcgtaaaaa aaataagaaa
    10741 aaggatgaga taaatccaga tgcatggctg gctacttatg cagatactat aacactgatt
    10801 cttacatttt tcgtattatt gtatgcaaca tcagttgtag atatggaaaa atttaaaaac
    10861 atagcagatg cgttaaaagg tcagttttct gggacaagta tatttagtac gggttcaagt
    10921 acaagtgaga aatctccaat agacagtgtt ttagtggata aaaatgataa atcaaattta
    10981 gaacaggatt taaaagataa agttaataat tctaacttac aaaatagtgt aacagtcaaa
    11041 gatgatagta gagggatttt actagagtta gatgattcaa ttttatttga ctctgggatt
    11101 tctgagttaa aagtagatag taaagctact cttaataaag tatatgacat gataaagatg
    11161 atgaaaaata agatagttat agaaggccat actgataatg tacctgtaaa atcaagtaca
    11221 catgaatcca attgggattt atcttctagt agagcagtaa gtgttgtaag atactttgta
    11281 gaagaaaagg gcatggaccc tagactattt tcggcaacag gatgtggaga gtatcaacct
    11341 ttggtttcaa atgaaacagc aaagggaagg gctgagaata gaagagttaa tatacttata
    11401 actattgact aggaggaaga ggtttgttca aaaataaaaa aattattata attattttga
    11461 tactgattat tttctcagcg ggagcaggtt tttttatatt aggagaaggt ggaaataaac
    11521 aagttgacaa gattgcaggt ttgtttattc caacaggaaa aggtaaagat gaagtaaaga
    11581 ccgaaaatct tgacttagaa gagatggtat taaagttagc tgatgaaggt gtaagatatt
    11641 taaaagtatc aataactgta tcttatgatt cttcatataa agatatagaa aaaaatacgc
    11701 ctttaattag ggataatatt ataaattgtt ttatggcaaa aagggcagat gattttactg
    11761 ctgataattt aaagaacata aaagaagaaa ttaaaaatac attaaatata caactaggtg
    11821 aaaatgtaat tcaaaatata tatttcacaa atatagtagt gcaataaaag gatggtaaga
    11881 catgatactt tatttactaa aaatattaat atgtatccca ataattttaa tattgatagt
    11941 cttatcttta aaaattagcc aaggaacttt attaaaaaaa tataatgata aatatgtaaa
    12001 aatactagat attgtaaata taagtaaaaa caatagcata ataattttaa aaataggtga
    12061 agaaggatgc attatggcat cttcttcttc tagcatgagt aaaataaaag atttgaccaa
    12121 agaagaagta ttaaaaatag aaaacgacat taaagaaaga aattttaaaa ttcaagattt
    12181 taaaatggaa agaattaaaa agaaatatga tttcagtaga tatattggaa aatttacaaa
    12241 aaaggaagat aagtaaatgg aactattatc aaaattacct ttggctgatg taccatatac
    12301 aagttcaatg caattgtttt tatttctaac tgcattaatg tttttaccat ttgtaatgtt
    12361 catgatgaca agctttgtca gaatagttat aagtttatca tttttgaaat cagcattagg
    12421 agcacagcaa gcaattccaa gtcaaatttt ggtaggactt gcaatagtat tgacaatatt
    12481 tataatgaga ccagtattaa atgagataaa cgaaaaggca ttacaaccat atatgaaaga
    12541 agaagttact atggaagaag ctatgaagga agcagaagga cctattaaag agtttttatt
    12601 aacacaaacc agacagacag atttagattt atttgtagaa caggctggtt taaaagaaaa
    12661 gaaattaact cgtgaaaata taccactatc tgttgtagtt ccagcatttg ctataagcga
    12721 actaaaaact gcttttcaaa taggtttttt aatatatata ccatttttga taatagacct
    12781 tgttgtggca agtgttttga tgtctatggg tatgtttatg ttgcctccag tcatgatatc
    12841 attaccattt aagttattat tatttgtaat ggtggatgga tggaacttga tagtaaaaac
    12901 attgatattg ggatttggat aggagaaaaa tagtatgagt gaatttatga taatgagtgt
    12961 tcttaaaaat ggcatgttta caggagctat gattgcagct ccagtactca ttgtttctct
    13021 tgtagttggg cttttaataa gtgtgtttca agctacaact caaatccagg agcaaacatt
    13081 gacttttgta ccaaaactaa tagctattct tttagtagta gctatcggtg gaagctggat
    13141 gttacatact cttattagtt ttacttctga gatattcaat atgatatctc ttataactaa
    13201 ataagtagga tacatatgat ttttgtattt attagaatag tagcattctt tggaacacta
    13261 agaatactat ttccagctgg tacacctgct ttatttaagt cactctttgc aatcattata
    13321 tcaatattga tttcaagtac tatgcaaatt gaatatgcaa caaatataga caacattgta
    13381 ttgtttacct tatatagtgt aaatgagacc ataactggga ttatgcttgg atatataacc
    13441 aacctatgtt tttatagtat cagaatggcg ggttctatga tggaccaaca gatgggatta
    13501 tcaatgatta atatgtttga cccaaattca atgactcaga caactttaat tgataatctt
    13561 atgaactgga cagcacttat gatatttttt aacatggatg gtcatcatgt tttaattaga
    13621 ggtataaggt atagttttga gttaattcca ataggaaaac catttgtaga taataacata
    13681 gattatataa taaacatatt tgtgcagtgc tttttaactg gattcaaaat agcaatacct
    13741 attgtacttt gtctgcttat ggcagatttt attttagggc ttatatcaag aagtgtacct
    13801 caattaaatg taatgatagt tggtatgcca ttaaaaatat tggttggtat tgcattattt
    13861 atcatatcaa tacctttaat tgtcaatcaa ataagtcatt tattaagtca aataccaaag
    13921 atgtatgagg gaacttttgc actagctcct atgtttttta tgggttcagc agataaaaca
    13981 gaagaagcta ctccaaagaa aaagggagaa caaagaaaga aaggtaatat agcaaagagt
    14041 agagaattac ccgttgcaat gactctactt gcattcacct tattagtacc tacattattt
    14101 tcatatgtag tagatacact taaatcttcg cttaattatt ttttgagtct agatttttat
    14161 atgaacataa attattcaaa cctagagaaa ttagttatag caggattaat ggattttttt
    14221 aagatatttt taccaatagc tattcccttt ttagttttag gcataatagc aaacctattt
    14281 caggtgggaa tactgtttac tggtgaaact ttaaaaccaa atttatcaaa attaaatcca
    14341 attagtggat tcaaaaacat gttttctatg agaagtttgt caaccttaat aaaggataca
    14401 gcgattatat ctattttggc ttacatagga tatacattct tccaagataa ttatttagat
    14461 atattaaagt taggtaacat atacctacca actttaatgt acacagttaa agacttagta
    14521 tattcaatat tgagtaagat ttgtgtagct atgatagcta tagcagttgc agattatgtt
    14581 tatcaaagat acagtcataa aaagcaactg agaatgacaa aacaagaagt taaagatgaa
    14641 tataaaaact cagaaggaga cccagaagta aaagctaaga taaaacaaaa acaaagacaa
    14701 atttcgtctc aaaggacaat gcaagcagta ccaagtgcta ctgtaatagt aaccaatcca
    14761 acccacttat ctatagcagt gagatatgaa aaagggaaag accaagctcc agtagtggtg
    14821 gcaaaaggtg cagattattt agcttttaaa ataagggaaa ttgcaaaggg aaatgacata
    14881 cctataatag aaaataaacc tattgctagg ttgttatata agcaagtaga aatagaccaa
    14941 gaaattccag aggacatgta tcaagctttt gcagagatat tagtagcagt atataaaatc
    15001 aaaaatagat acaaagtccc caaaaggtag gataagtaat gaataagttt aatttaaagg
    15061 aaaatatgga cgtaatagtg gcatttggtg ttgtagggat tgttcttatg atgatagtgc
    15121 cgttacctac atttatatta gatattttac tggcaattaa cataagttta tctgtgctaa
    15181 ttttactaat gacactattt acaacaaatg tacttgattt atctatattt ccaacagtgt
    15241 tacttgtgac aacactattt agattgggat taaatctttc atcaactaga ttgattttaa
    15301 cacagggata tgcaggtgaa gttatagagg catttggaag ttttgttgta ggtggaaatt
    15361 acgtagtagg aataattata ttcttaatta tattaattgt tcaatttgtt gttataacca
    15421 atggttctgg aagagtatct gaagtaacag ctagatttac gttagatgcc atgccaggta
    15481 agcagatgag tatagatgca gatttaaatg caggagttat agatgacaaa actgctagaa
    15541 aaagaagaaa agagcttcag caagaagcag atttttatgg ctctatggat ggagcaaata
    15601 agtttgtaaa gggagatgcc atagcaggtc ttatagtaac agcaataaac ataataggtg
    15661 gaattgtaat aggagctgta atgctgaata tgactcttat gggtgcagca cagacatatg
    15721 taagacttac tataggggat ggtcttgtaa gccagatacc tgctctatta atatctacat
    15781 cagcaggtat aatcgtaaca agagcttcta ctgatgaaaa ctttggtgga ttagtaggta
    15841 aacagttaac agcaatacca aaagcagtaa tgatgactgg ctgtgttcta atcgtacttg
    15901 gtatgatgcc tggattacct aaaccagcat ttttttcact aggtataggt gctatagcag
    15961 caggttactt tttaggaaga gaaaagaaag atactttaga agacttggag tttgaaaaac
    16021 ctgatatggt aaatccagat aaaaatgaag tagaagatgt aacaaactta ataaatgtag
    16081 aatcaataga agtagaaatt ggatatggtt taatatcttt agctgatgaa tcagcaggag
    16141 gagatttact tcagagaata gcatcaataa gaagacaatg tgcaatagac atgggtatag
    16201 tcgtacaacc tataagaata agagataatt tacagttaaa tccaaaccaa tacaatataa
    16261 aaataaaagg gaatgtagtt ggtgggtatg agataatgcc taatatggtt ttatgtatgg
    16321 atcctatgaa tcaaggattt agtatatttg gaataaagac aattgagcct acttttggaa
    16381 tagaggcact ttggatagaa agtgacaagg tagaagatgc agagctaaag ggttatacag
    16441 ttgtagattc atccacaata cttataactc acttactaga tataataaaa tcaaatgcac
    16501 acgaattact gggaagacaa gaagttaaga ctattataga tgcagcaaga gaaaattata
    16561 gtgctgtagt agatgaattg ataccagatt taatgacact tggtgaaatt caaaaagtac
    16621 ttaaaaatct gttaagagaa aaagtaaata taaaggatag agttacaata cttgagacac
    16681 ttgcagacca gtctagaaat acaaaagata tagagcttct tactgaatat gtaagaatag
    16741 ctttggctag aagtatttgt aataatttag ttgatgaaga taaggcaatt gtagtggcta
    16801 cattgtctcc agaaactgaa gaattggtat ctaataattt acaaaggtct gtaaatggaa
    16861 cttatccagc agttgaccca gaaaatacaa ataaaatatt tgagagtata caagaagtaa
    16921 tgaacaatgt atatttcaat cataatatac cagtaatagt agtatcacca aagataagag
    16981 ctccatttag aaaattagtt gaaattgtat atcctaattt gactgtatta tcattaaatg
    17041 aaatacctaa cgatattaaa atcaaagcag aaggtgtggt gagcatatat gatgacgaaa
    17101 aagtatacag ctaacacaat tcaagatgct atgaatctag ccaaaatgga attgggtgac
    17161 aatattacat taatagataa aaaagaggta agaaaatctg gaataagagg cattttttca
    17221 aagaaagata ttgaattgac tattggatgg gaaaagaaag ataatttaga acaaaaagat
    17281 ttaaaacgag agatagaaca actaaagtca attataaata atatggggtt tgataataaa
    17341 aatgataatg atatagataa aatatgcaag aatttactaa acttagattt aaatcaagaa
    17401 attgtagaat ttataaaagc tgatttacaa gaaatgaagt tcaatggaat tgatacaagt
    17461 aaaaatttag tagaaagtct aaagaaaaag attaaaatag aaaatcaagc aataaatggt
    17521 aaaattgctt tagttggacc accaggagta ggtaaaacga ctacaatagc aaagttggct
    17581 gccaaattag tttttgagga aaataaaaaa gtaggagtaa taactataga tacatacagg
    17641 attggtgctg tagaacagtt aaagatatat actgatataa tgaatattcc ttttaaaggg
    17701 gtaataagcc ctgatgagat ggaattggca cttgatgcga tgaaagattg tgatgttgta
    17761 ttaatagata caacaggtag aggctataaa aactctatgc aaatattaga aatcaaaaat
    17821 cttatagata aagctgaaac tgataatata catttagttt tgaactgtac aacaagagaa
    17881 agtgatacaa aagcaattat agattcatat agaaatgtga attttaaaag cttaataatt
    17941 acaaagttag atgaaacaat aacatatggc tcaatattta acataatgaa ttatgcacaa
    18001 aaaccaatat catatattac aacaggacaa aatgttcctg atgatattat aaaacctaat
    18061 gaagataaga ttataagatt actattagga gttgaaagta tatgatagac caagctcaaa
    18121 ttttgagaaa aatggcaatg gaaaaaagag gttctgatga gattatagta gagaatgaga
    18181 atatgcctaa gataattaca atagcttctg gtaaaggcgg cgttggaaag agtaatctcg
    18241 ctacaaattt gtctatatgc ttaaccaaac ttgataaaaa agttcttata ttagatgcag
    18301 atataggaat gtctaatata gatataataa tgggggtaaa tgtaaaaggg actataattg
    18361 atgtaattaa tggcgaaaaa aatatagaag atataatttc acagactaag tatggtgtaa
    18421 atgtaatatc tggaggttct gcccttaatc atatagaaga ctttacagaa gctcaaagaa
    18481 ataagtttat acatagcata gaacagattc acaatgtgga ttttataata attgatacag
    18541 gagctggtat gagcaaaagc ctattgtcat ttatatactg tagtactgaa tttttcttaa
    18601 taacaactcc agaaccgaca tcattgactg acgcatatag tttattaaaa gctataagta
    18661 attttggcat aaagaaaagt gcaaatataa taatcaatag ggtaattgat ttagaagaag
    18721 cgaaatctac atacaaaaga ataaaaatgg ttgtggacaa atttctaaac cttaatctgg
    18781 aattgtatgg atatataata gatgacaaaa aagtaagttt atgtgtaaaa aaacagatac
    18841 cattcatttt agaatatcct acaagtattg catcaaaatg tattatagcc atagcaaaga
    18901 gaatggtaag tcagtcaaga gaagatattg ttgataataa aagttttcta agaaagatgt
    18961 ttagtatatt taaaagctaa ggaggcgtag ttaatgaata gagaagaatt aataaaagag
    19021 aatatgcctc ttgtaaagag tatagcaaga aaatttttta taccagggaa aggttttgaa
    19081 tatcaagact tagtaaatag tggagtcatt ggattgattg atgcaataaa taaatttgat
    19141 gctgaaaaag gggcaaagtt ttctagttac tcttatataa aaataaaatc tgctatatta
    19201 gatgaaatta gaaatcaaag tccaatttct aaacataatc taactaaagt aaataagtat
    19261 aatagagtgg tagaaaagtt gcaatctaaa ttattaagag agccaacttc atatgaaatt
    19321 gcaaaagaat taaaggtttc agaaaaagaa cttcatgata tcgaaagtaa tatagatatg
    19381 ttaaatattg tatcattaaa ctatgtaatc tttgaagata caaatgaaac tgtacaagat
    19441 gtaataagtg atagagaaga ggaagctcca gaaaatataa tagaagaaga agaaaagtta
    19501 gagattctat ccaaagcaat tagtaattta aatgaaagag aaaaattaat actttcactg
    19561 tactattatg aagatttaaa tctaaaggag atagggaaag tgctaggtgt ttcagaatca
    19621 agagtttcac aactccacag aaaatcaata agaaacttaa gaaacaagat aaaagaactt
    19681 aaatattcta tatagatggt gattgataag atgatacaaa ttatattaat agtagtgtct
    19741 attttaataa tagtaactat agttataaat ttacataagg aatcaaaaaa taaagataac
    19801 agatactttg ataaaatatt tgagcaagaa tatgaagcaa atgatagatt aataattgca
    19861 gaaaaaagaa gattcttttt agacaaagta cttgctgaaa taagaagtaa taatcatata
    19921 aaagtttatg aaaattataa cctaataaag gctaatgaag aaagaaagga aaacatattt
    19981 atagaatcaa aaaaggaagt aagacaagaa aatgagttat ctcttaaagt gaaacatctt
    20041 ataagttcag gttatgatga tgtggaaata tgtaagatgc tagatattgg aaagggggaa
    20101 ctgtcattaa taaggagttg ttacaaaata taaggatatt attatgtgat aaaaaagtat
    20161 tcttaggaat aggtattggt atattaatat caactttatg tataatgctt tttagtaata
    20221 atgatatgag taaggcagag atagaaaaaa aggccagagc gttaggcatg aaatatacaa
    20281 gtgagatgaa agtcttagaa tagatgatac tgtttagaca tttacaaaaa aggaggtcat
    20341 aatgtgataa gaggattata tacagcagtt tctgctatga taactaatca acaaaagcaa
    20401 aatgttgttg tcaataacct agctaatatg gatacaaatg gatataaaag taaacaactt
    20461 ttgactaaaa gttttgatga attaaagttg gaaggttaca ataactacgc aaatggaact
    20521 aaacaaaagc agattatagg cgatataagt ccaggtattt ctatggatga aactatcaca
    20581 aattttaatc aaggacctat aaaaactact gataataaaa tggatgtagc tattcaaggt
    20641 aagggatttt ttctagttag tgatgcagct ggaaatcaat tttacactag aaatgggaac
    20701 ttcagaacag acaatcaagg tgaccttata acaagtgaag ggtattatgt acttgggacg
    20761 aatactgcta ctggtgctgt aggtcatata aatgtaggaa atcaaaaatt tgaagtggca
    20821 aaagataata caatatcact agacggaaat gctatgtata aatttaatat tgtagatttt
    20881 aataactaca ataacttaaa aaaagaagga gataacttat attctggagg aggagctatg
    20941 aaagtaaata atgcacttac aatacaaagt gcgatagagt cctctaatgt agatatggta
    21001 tctgaaatta ataatatgat gacaatatca agagaatatg aggcaaatca aaagataatt
    21061 caggctatgg attctaaatt agcaaaaata gcaagtgaga ttggttcagt aagataggta
    21121 ataggaggtg aatttttatg tacacaatgt tttatactag taaaacagct ttatcagcaa
    21181 atcaaagtaa actagatata atatctaaca atatagcaaa ttctgattca acaggatata
    21241 aaaaaataaa tatggaatat tctgatttag tggatgaagt actaaataga ccatcatacc
    21301 ctacaaatgg taaagatata tcgactggaa caggagctaa ggcaacagct cccatgagaa
    21361 attatgaaca aggtgcattg gctcctacag atagtaagag taatttggcg atagatggag
    21421 aaggattctt tagagtgata agaaaagatg gtacatatgc atatacaaga aatggtggat
    21481 ttaatgtaga tgctttaggt aagattgtag atgataatgg aaatatatta gatgtccaat
    21541 ttgatgcagg atataactat aataatacaa atatagatag tacaaacctg acagtttcaa
    21601 gaaatggaga gctattcgta gataataaaa aaataggaaa aatcaactta tatcaaccta
    21661 taggaactca aaattttata tctgaaggag atagtttatt cgtcgcaact gatgcagcac
    21721 agattaaaca agttgagaaa gtcgatatag ttcaaggcta tagagagagg tcaaatgttt
    21781 ctttacaaga agaatttgtt gacttaatag ccactcaaag agcatttcaa atgaactcaa
    21841 agggaataaa gacagcagat gaaatgtggc aaatagcgaa taatttaaga gctaaataat
    21901 tatgtaaggt gaggaatatt catgaatgat atgaaagagt acgattttgg caaacctcag
    21961 agttattcaa gagaaaattt aacttgttta aattttttat tagctgaatt ttgtaaaaaa
    22021 tataaaaact atataattta tgaacttaag tgtaattcta gtatggttgt tgatagtata
    22081 gaccaagtaa attatcagac ttttttagat agagtaactt cttcgtgcgt agtagtacaa
    22141 aatgcaatgg aacctgttat gaaaaacttt tcttttagaa tagatagaag tgtagctgat
    22201 atgtggattg atataacttc tggaggaact ggagttgtaa aggataatga tagagagctt
    22261 acagaacttg ataaaaaagt gcttttacac cttatggatg atttagtaag aaatatgcac
    22321 ttatttgagg gttttgaaaa tatacaagta ttggatacac atacacatat taatttacca
    22381 caattatgtt ctccaactgc accagtatgc gttgtagatt taaaagtgct aaatgataat
    22441 gagtatattg gaagtgtttc tctttgtttt ccatacaatg gtttagaagc tctgttagaa
    22501 agtatatctg ttatagaatt ttttgataat gatataggag atgattcaga ggaatttacc
    22561 aaaaaaatat ataatagtgt atctaatata gaattatcgg ttattgcaga gataggaaaa
    22621 gtatcaatta atgttgaaga cttacttaaa ctagaagttg gagatgtaat agtaacaaat
    22681 aaaaaaataa atgattatat agatatattt gtagaaaatt caaaatcata tacagcaaca
    22741 ccaggattta tatcaagtaa aaaaggtgta aaaataaatg atgctgttgg aaaagaggtg
    22801 tagttatgga caatagtaat cttatttcag atgaagaagc aaagatgcta ttaaatgata
    22861 aagttgaaag tgaagttgat ggagatgatg agttagcctt agatgtgtct gaagttaatc
    22921 gaaataatat acaaaggata cttgacctta agttagaatt atctgtggtt ataggaagaa
    22981 ctaagatgac tctaagagat attttaaacc tacataaagg ttctcttatt gaactggata
    23041 ctttagcaga ccaagatgtg gaaatactaa tagataataa agtgttggct tatggaaaag
    23101 tagtggtggt agatttaaat tttggtgtaa agataacaag tatagtaagt gaagaagata
    23161 tggtaaaatc tcttgtttag agataacatc aattaatact taaggcatga ggtggtactt
    23221 attttaaact taaatgatat aaaaaaagat atattaaaac tcaataaaaa agactcatgt
    23281 tctctatata atgaatctat taataatata aataataatt gtatatcgag tggattgaaa
    23341 tttttaaaac aagcaagaga tttaaaccca gatgatgttg atatattaaa tctcacaggt
    23401 cttgtaaatt tactaaaatg taattttgat gaggctgtag aaagtttcta taagagttca
    23461 ctttgtgaaa aaactgctct gtgtgaaaag tatataaaca tgcttacatc tgaggagttt
    23521 ttaattttct ttgaaaggta caatcaactc ataaggttaa taaacagagg aatgtataaa
    23581 gaagctatac aagggtttaa acttataact gagcaatact gtgatttaat agaaccttat
    23641 gaattgcttg ccttactgta tgacaaggaa gaagaatata taaaatttga tgaatgttta
    23701 gaagtattga agattattga taaggaaaat tatttattaa atcaagaaaa taacacatta
    23761 gatatgtata atagtgctaa tatacaaact aaaagtaata taaaagtaga tgattataag
    23821 aatgagttct tacataaaag tagtagtaac cataaaatta atgattataa tgatgtaact
    23881 aaagaaaaga gttcaagcaa aaaccagaaa agttcagatt ctaataagaa aaaaagtatt
    23941 aaaaaaaata aatacctata tatagccata atattaggaa tattattagt aggacaaagt
    24001 atatatagtt cttataagat gaataattta actgataaaa tatctaaaaa taatgaaacc
    24061 aaaaatgaag agtcaataga ttctaacttg actaagaaag aaaataatat agaagaaaat
    24121 gaagatatga gtaaggcttc taatataaat aagagtaata aaaacattaa taaagaggat
    24181 gatgtaaaat ctaaaaaaaa tactgtagaa aatgaagatg ataaagattc tgacttattt
    24241 acagaggatg aattgatgtc taaggcgaat actttaaaat cagaaaagaa aactaaatct
    24301 tctatacaac tatataagaa agttgcagat ataggtgaaa ataaagggaa tacatctgaa
    24361 gcaacttatc aagttgctat tctaagtgaa aagttaaaag actataaaac tgctgaagaa
    24421 tattataaaa tgtatgtaga aaattactca gaaaaagatg cttattttga tgaatcttat
    24481 tataatttag gtatgatgta ctataacaat ggtgatttaa aaaattctaa attgacatta
    24541 aagaagttgg ttaataaggt accaaatagt atgtataata attcaaaagt aaaagaaatt
    24601 ttaaaagaag aatagttaaa ttagataaga aaaataaatt attttatttt aaaataatat
    24661 gattataata tattgacata ccattttaaa aaatttaaaa tattaattat aaaatgagga
    24721 gggataaaaa atggaatttg aaaaagggag tatttcaatt catacagaaa atatttttcc
    24781 tataattaaa aaatggttat actctgataa agatatattt ataagagagc ttataagtaa
    24841 tggatgtgat gcagtaagta aacataaaag acttgtatca ctaggagaaa tatcagaaaa
    24901 taagtcatca gattataaaa ttacagtatc tgtaaataaa ggagaaggta cacttaaatt
    24961 tattgacaat ggaattggta tgactgaaga agagataaaa aaatacataa atcaagttgc
    25021 tttttcaggt gctgaagatt tctttaataa atataaggat aagatggaag agtcaaatga
    25081 cataataggt cattttggat taggatttta ttctgcattt atggtgtcta aaaaggtaca
    25141 aatagatacc ctttcatata cagaaggtgc aactcctgta agatggatat ctgaaggtgg
    25201 tacagaatac gaaatatctg aatcagatac tagaaatgat agaggaacta ctataacact
    25261 atttattgat gatgatagta aagaattttt agatgaattt actgtaagag gaataataaa
    25321 taaatactgt tctttcttac cagtagaaat atatttagaa gatgttgaaa gattagaaag
    25381 agaagctaaa gaagcagaag aaaaagctaa aaaacaaaag gaagatggaa aagaagagat
    25441 tgtagatgca aaagtaattg agcctttaaa cgacactaat cctctatggt taaaatctcc
    25501 aaaagactgt actgatgagg agtataaaga gttttataga aaggtattca atgtatttga
    25561 tgaaccttta ttttggatac atttaaatgt agattatcct ttcaatttaa agggtatact
    25621 ttacttccct aaattaaaaa atgagtttga actgactgaa ggaaaagtaa aattatacaa
    25681 caatcaagtg tttgtagctg acaatataaa agaagttata ccagaatttt tacttttatt
    25741 aaaaggtgtt atagactgcc cagacttacc attaaatgta tcaagaagtt tcttacaaaa
    25801 tgacagagac gtaagcaaaa tttctaagca tataattaaa aaagtagctg ataagttaaa
    25861 atctctttgt aaaaatgaaa gagaagaata caataagttt tgggatgata tacaaatatt
    25921 tattaaatat ggttgcttaa aagatgaaag tttctatgag aaggtaaaag aatgtatatt
    25981 attcaaaact attgatgatg agtatataac attacaagat tatttagaaa aatgtaagga
    26041 caaacatgaa aataaggtat tttatgtaag tgataaggaa cagcaatctc aatacattaa
    26101 actatttaaa gaatacgact taagtgctgt tgttttaaac tcatcaatag atactcattt
    26161 tatttcattt atggaatata aagagaatgg agttaagttt aatagaatag atgcagacct
    26221 ttctgatgta ttaaaagata aaaatgaaaa taaagatagt gaagaaaaca aagaagagat
    26281 agctaagata gaaggtctgt ttaaagaagc tgttggagaa agagtgaagc attattctgt
    26341 agaaggattg aaaaatgaag acactccagc tatggtgtta gtatcagaac aatctattag
    26401 aatggcagaa atgcagtcaa gatttgctgg aatggattta ggaatgaatt ttgaggaaga
    26461 aaaaactctt gtaataaatg agaacagtcc aataatcaaa aaattggttt ctcttaaaga
    26521 tgatgaggaa aagaaagata agattacgct tatttgtaac cagatagcag atttagcttt
    26581 actttctaat aaagaattaa agccagatga gttagattca tttgtacaaa gaagtaataa
    26641 gcttatgagt cttttcatag aattataaga ttgaatttga atacattgaa gcaaatttat
    26701 aaaaagtatt caatatcagt gatgataaag aagtgtctca aaatgaactt ttcagttcat
    26761 ggggcgcttt ttttatgtga aattaaattt attttaatca tttcaacaat gaaaaatata
    26821 tttatctcta ttttgagaca gaccattctt tattttttta tctttggtta atttttaaat
    26881 atttaagtag gttctaataa aaaactctat ttagttaaat atagttaact tgatatataa
    26941 taagataata catataatta agtaaaagat tgttttttag tttatgttta aatacataat
    27001 tacactttgt taaaaacata aaattattac taaagtagaa ataggaggtt ttagcatgaa
    27061 ttttaattat aatttgccag taaatatctt atttgggaga ggcagaatta atgatgtggg
    27121 aaaagaagta tctaaatatg gcaaaaaagt attgatagta acaggtaaaa atagtacaaa
    27181 gaaaactgga ttacttgata aaacaataga tttactaaaa gactctaaaa tagaatatga
    27241 agtttttgat agagtagaac aaaatcctct tataactact atatattcag gagtagaaat
    27301 tataaaatca actggatgtg attgtgtttt agggcttggt ggtggaagta ttatggatgc
    27361 tgccaagagt atagcttttt cttataaaaa ttcaggagat ttaaatgatt atatatttgg
    27421 aattagacaa ggtgaagaag ctttacctat aatcttagtt ccaacaacat caggtacagg
    27481 tagtgaagga aattgttttg cagtacttac aaattcagaa actaaagata aaaaatctat
    27541 aaagaagaat agtatgtatg caaaagcatc aataattgac ccagaactta tggttacaat
    27601 gccaaaacat ataattgcat cagttggatt tgatgcactt gcccataata tggaagcata
    27661 tctttctaat ggaagaaacc ctcttgcaga tgtacaagct atttatggga ttgaacttat
    27721 atcagaaaac ctaataaagg tatataatga tgttaatgat ttagaagcat gggaaaagat
    27781 aacacttgca agtactattg gaggaatgac gattggtaca gcgggtacag ccttgccaca
    27841 tggaatggag catcctgtaa gtggtcttag agatgtaatg catggaaaag gacttgcagc
    27901 tttgactcct actattatag aaagttcatg ggatagtgat attgaaagat ttggtaatat
    27961 ttcaaaggta ttaggaggaa cttgtgcaga agattgttca gatgcaatta gaaatttcct
    28021 taaaaaaata gatttagata taaagctaag tgatttaggt attgaaaaat cagatataga
    28081 ttggttaact gataattgta tgaaaatatc agttgcaaat attaagagac atccaaagta
    28141 ttttaataag gaacaaataa aggaaatgta ccataagtcc ttataatttg actttacata
    28201 tttagtttta tataaaaatt ataaattaat aataaaaaat tataagtaat taatagtaaa
    28261 aaaattgtgt atagaagtta tatatataat aaatttgtct taagctttta ttttactttc
    28321 taataaataa aggagtttaa aaaagggaag attaattaag taatatttta taaaattctt
    28381 cgatttaaaa actttaagta gaggtgaaat aaatgtttaa tcccaaaaga ataatattcg
    28441 aaaaaggaac tttagacact gatgtgggta aaaaaatata caataaattt agagataata
    28501 aagatgtaga gataattaat atatcatcaa ataggattaa agaacatatt ccaggtgaaa
    28561 atctatatga acaatataga agtgggaagc agactatggt agttgggatt agaaaaagtc
    28621 tgaagtttca aacatgtaaa ccttctgcta attatcaact accacttgtc agtggatgta
    28681 tgggaagatg tgagtattgt tatttgaaca cacaattagg tgataaacca tttataaggg
    28741 tatttgtaaa tgtagatgaa atacttgaac agacaaaaaa atatattgaa gatagaaagc
    28801 cacaggtaac catatttgaa ggggcagcta catcagaccc agttccagta gagccatata
    28861 ctaattcttt gaagaaaaca atagagtttt ttggaagaga ggaaaaagga agatttagat
    28921 ttgttactaa atacagtgat gtggatagct tattggatgc tgaacataat aatcatacag
    28981 aaatacgatt tagtatcaat actcctagaa ttataaatga acatgagcat tatacagcct
    29041 ctgcaaataa aagaatagag tcagctgtaa aacttgctag ggctggttat cagattggtt
    29101 tcataatagc acctgtgttt ttatatgaag gttggaaaga agagtataaa aatctgatta
    29161 taagtataaa agaagcttta ccaaaggatt ttgataaaaa aattatattt gaagttatat
    29221 ctcatagata tacaactaaa gcaaaaaata gaatactaga gatttatcca gatacactat
    29281 tgccaatgga aaatgaagat agaaagttta aatacggtca atttggatat ggtaagtacg
    29341 tgtatgataa agaacaatta gatgatatga aagagttctt tcttaaggaa ataaatggag
    29401 tatttaaaaa tagtttaata aaatatataa tatagcaaat cttacctaaa gatttgctat
    29461 attttatttt tatatatttt atgtttataa taaagcaatg agatttagca ctatcatata
    29521 agaaaattaa aaagggcact atctatcaag aacatttttt ctagttttga tactataaag
    29581 gataagagta acaaaaatat caggaggagt tatagtatgc atgcatggga gtctattcaa
    29641 attacattgg atttaataga agcaaattta tcagaagaaa tatcaattaa tgaattagca
    29701 aataaagcaa atttatcacc attttactat caaagactat ttaaaagatt ggtaaataag
    29761 actgtaatgg agtatataaa gcttcgtcgt cttgcaagag cttctgaata cttagcagaa
    29821 cataagaatc gtatacttga tgtagcttta aattttggat ttgctagtca tgagacattt
    29881 acacgttcct ttaaaaaagc atatggatta actccagaaa aatatagagc aaatccagta
    29941 aaactcaatc actttattaa gccagaatta attttaaatt atgctatggt tgatgaaggt
    30001 gttccactga ttgcagatga tatagtgatt gaagtcaatc gtaagacatt gagtaatcca
    30061 aggacatttg ttggaataga aatagaagta cctatttgcc agttggtggg cggtgaaaca
    30121 actggaattg caatagtaga ggaattatgg atgaaacttg gaagtcaaag acataatata
    30181 ccaaatcaaa ttccaggtgg aaatgaattt gctgctcttt atatgggaaa tgcaaaagaa
    30241 ggaaattgta tgtatatggc aggtgtagag gtagagtcag gtacatcagt agaaggatat
    30301 agtacttttg agttgccagc taaagaatat ttagtatgtg gatttgaggc agaaaacttt
    30361 aatgagttag ttaattcagc tgtttttaaa gcagataaat ttatggaaag atggatgaag
    30421 aaacataatt taacaactac tgattttgca attgagatgt attaccccac tacacctgaa
    30481 gcagcatatc tggaacattg gattgttccg gttcctattg aacaatagaa aggatgatga
    30541 atatgaattg gcaagatgcg ttttttcaag atactaaacc tacatttgag caaattaatg
    30601 aatttattaa taatccatta tgggacaagt taaataaaac tttggtttct acttataatg
    30661 tagaacctaa gcttgaatac agtaaatgtt caatgcaaag aggttggaat gtaaaatata
    30721 aaaaaagagg taaatcacta tgtactttat atccacaaga aggttatttt aaagctttgg
    30781 ttattgtaag tgaaagcaat agagtagagg ttgatttatt tattaatact tgttgtgatt
    30841 atatcaaaaa aatatataat gaggtaaact tttttaatgg aagtaaatgg ctaatgattc
    30901 aagtagatag tttattggta ttaaacgata tgcttgaact aattaaattt agagcttaac
    30961 atatagaaca ggatgaatat ataagtcatc ctgtttttat attcaatcag ataaattaat
    31021 atatagtcta taatatgaat aatttagtaa atttaaattt actttatctt tctacattct
    31081 ttgcaactaa gtccacatgt gtttttttct tgcatattag tagcacccca attagcaaat
    31141 tgttgtaaaa taggcacaat acttttacct ttacttgtta atgtatattc aacttttgga
    31201 ggaatttcat tatattgttt acgatgaaca ataccagtag actctaaatc ttttaatgat
    31261 tgagctagca tagtatttgt aatgccatcc aattttcttt ttatgctact atatctaatt
    31321 ggattttcaa ctgctaacat acatataata ggtaatttcc acttaccacc gacaatgtct
    31381 aatgcataac ggattggaca ggttaattct tcaacgtttt tagttatatt tttttcagta
    31441 tttatactca ttttaattac tccttagtta tagtagtatc ttttttatac tttatcagaa
    31501 aaaactgcat acttgttttt tctgtatttt atattaaaat tatatcaaag gataatcctt
    31561 aatacaagta tttaaaaggg gtgtaagaat tatgaaagca ttagaatttt tgaacaaggc
    31621 acgagtttat tatttagcaa cagttgatga gaacaatcaa gcacatgtgc gtcctttagg
    31681 atttgttatg gaatacaatg gtaaacttac attttgcaca tctaatcaaa aggatatgtt
    31741 taagcaactt atttttaatc caaaggttga actttgttgt atagaccaaa accttaatac
    31801 tctacgtatt ttaggtgaag cagtatttgt tacatcaaaa gaaacacaag ctaaggcact
    31861 tgaaataatg ccaagtttag gtcagatgta ctctgtagga gatggtaagt ttgaaatatt
    31921 ctctattgat aaggcaaaag ttagttgttg ttcaatgagt ggtaaaaaga ttgatgtaga
    31981 gctttaatta taattgaatc gacatccata gtgaaaatta agaaaacgtt attactgaaa
    32041 tgttgataga tttaatttaa atgcaataaa aagattttta aataatgaat atgtaaaaat
    32101 ttttagttat tagatgtgta tttcaataat gatatactat tttgaataat tacatactag
    32161 aaataagata attaaaattt actttataat gggatagatt tcttttaaaa aatattttaa
    32221 ctataattga tttttattta atctgaaaaa aatattatga ctataaaaaa ttgctactta
    32281 ttatttggat atttttatat ataaataata agtagcatga ctttatacat taataatgat
    32341 agaatataaa ttaatagata agatatgatt ttaaaaggag tgatataaga tgagtaaata
    32401 tagttcttta tgggaatatg ttaagaaaaa caatagtcaa tcgtttaaat taacttttga
    32461 agaaataaaa gatattgctg ggatagagat agaccattcg tttttgaaat ataaaaagga
    32521 attaaatgaa tatggatatc aagtaggaaa aatctcttta aaagagaaga ctgttatttt
    32581 cgataaaatt ggttaagaca taaataatta catataataa aataatttgt attgatgatt
    32641 attacaaata aaaaagggcg tagcactatt ttttagtgtc acagcccctt ttttgtttat
    32701 aattatgtac atttcacatt atcacgatat aagcattttt cttatgagaa aattaattag
    32761 aattattttt attcatattt aaattattta aattgttttc ccagtttttt atctcctttt
    32821 taacttcttc acttaaagta tcacttgaag atattttctt taatttatca ttcaaagatt
    32881 tcttttgttc ctctgaaagg tctgttttta ttgtaggatc aataatatct attacatctt
    32941 ttacatcaaa atcaacaaat ttgcacagtt cttctagatg atcaattaga aaatctagac
    33001 cttcatatga ccattcttta taatcttttc cgttttttaa tagaaactca ttcacttggt
    33061 aattctgatt tgctaattca aagaatgaac ctgaaggatc cattatatca tttatagagt
    33121 tttcatagtt aacaatatta atttgtctta ttaatttctc tttttctgtt aagttgctat
    33181 aattcttgtt aacaaaagct actaatccaa attcaaagtt taaaattgaa tctttacgtt
    33241 tttttactga atcttctcca aattccctaa gaccttctaa atttttatca atggctttat
    33301 ctctaatctc tattaattgt tctatactta aaccttctaa gtttttaggt actatttctt
    33361 cattattctt agtaacttct ttcaatttat tgttagtatt tttattattg aaaataaata
    33421 tagcagttaa taaacttaaa attattacta aaataattaa acttacattt tttttactta
    33481 acatattaaa cctccttttt taaataagta ttgtacttgt gaaaaataaa tttgtttatg
    33541 aagataacat ttactactgt ttgttctaaa gattataata gaatattttt ttg
//
LOCUS       DAENCV010000036        33365 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAENCV010000036 DAENCV010000000
VERSION     DAENCV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.036"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(68..1867)
                     /locus_tag="KQD82_001679"
     CDS             complement(68..1867)
                     /locus_tag="KQD82_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG4380153.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNSSSASKITLSILNDKKIYNP"
     gene            complement(1870..2256)
                     /locus_tag="KQD82_001680"
     CDS             complement(1870..2256)
                     /locus_tag="KQD82_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380154.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYTPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVAKESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            2489..3166
                     /locus_tag="KQD82_001681"
     CDS             2489..3166
                     /locus_tag="KQD82_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4380155.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSKV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            3142..3603
                     /locus_tag="KQD82_001682"
     CDS             3142..3603
                     /locus_tag="KQD82_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380156.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            3671..4450
                     /locus_tag="KQD82_001683"
     CDS             3671..4450
                     /locus_tag="KQD82_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG4380157.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVVEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(4696..5322)
                     /locus_tag="KQD82_001684"
     CDS             complement(4696..5322)
                     /locus_tag="KQD82_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG4380158.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(5465..5722)
                     /locus_tag="KQD82_001685"
     CDS             complement(5465..5722)
                     /locus_tag="KQD82_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380159.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            6048..6560
                     /locus_tag="KQD82_001686"
     CDS             6048..6560
                     /locus_tag="KQD82_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380160.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            6815..7519
                     /locus_tag="KQD82_001687"
     CDS             6815..7519
                     /locus_tag="KQD82_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG4380161.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            7516..8286
                     /locus_tag="KQD82_001688"
     CDS             7516..8286
                     /locus_tag="KQD82_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG4380162.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            8283..9062
                     /locus_tag="KQD82_001689"
     CDS             8283..9062
                     /locus_tag="KQD82_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG4380163.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            9114..9806
                     /locus_tag="KQD82_001690"
     CDS             9114..9806
                     /locus_tag="KQD82_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380164.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            9825..11249
                     /locus_tag="KQD82_001691"
     CDS             9825..11249
                     /locus_tag="KQD82_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4380165.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNNINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(11489..13957)
                     /locus_tag="KQD82_001692"
     CDS             complement(11489..13957)
                     /locus_tag="KQD82_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380166.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIGLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13960..14628)
                     /locus_tag="KQD82_001693"
     CDS             complement(13960..14628)
                     /locus_tag="KQD82_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380167.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(14914..15441)
                     /locus_tag="KQD82_001694"
     CDS             complement(14914..15441)
                     /locus_tag="KQD82_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG4380168.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(15658..16818)
                     /locus_tag="KQD82_001695"
     CDS             complement(15658..16818)
                     /locus_tag="KQD82_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4380169.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(16806..17480)
                     /locus_tag="KQD82_001696"
     CDS             complement(16806..17480)
                     /locus_tag="KQD82_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380170.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            18569..18805
                     /locus_tag="KQD82_001697"
     CDS             18569..18805
                     /locus_tag="KQD82_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HBG4380171.1"
                     /translation="MRTIFAEYNPQCNSIDVYTNTGYILRIDCLEAEKNLRTTPGSDC
                     ALTSLAIDEPLEYARLYLDGNLQMWVDAEDSLEL"
     gene            complement(18939..20258)
                     /locus_tag="KQD82_001698"
     CDS             complement(18939..20258)
                     /locus_tag="KQD82_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter MatE"
                     /protein_id="HBG4380172.1"
                     /translation="MNHKSLFFKYVIPSIIAFALSGIYAIVDGYFVGNTIGDAGLSAI
                     NIAYPIEALIQALGTGIGMGGAVYYSINAAEKKGKRAEEFIATSWWLLVIVSVISTII
                     IYSFSSKILGLLGADGIILTNATDYIKIIALGAILQILGTGMMPFIRNYGNSFWSMFA
                     MVGGFITNIVLDFIFVWIYEMGMKGAATATIAGQGVTAAIAIIYALYIKKFYVYVSLK
                     NVKEMCGAIFRVGLAPFGLALTPNISLVFINRFSASYGGEKAIATYACVSYIICIIYL
                     ILQGVGDGSQPLMSRFYGEKDQESLRGVQRMAYIFAIILAVCGGIIMYVNRANIGGVF
                     GASYEVSIEISKIVPIFLVSLPFVAITRIATAGFYATEKSGLSYILTFIEPVLMLIFM
                     LVLPPLFGGQIMIWWSMVLARVFSAILAFILIKYCEKGDLQYGIQKI"
     gene            complement(20248..20706)
                     /locus_tag="KQD82_001699"
     CDS             complement(20248..20706)
                     /locus_tag="KQD82_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380173.1"
                     /translation="MRKDNNMIIQLQRYYALWKECTAMYEEWSKDQGLPSNGVFALHS
                     FYESNGRCTQKMISEKWNIPKQTVNTILKDFQKKGYINMVSDDSDKRNKLICLTESGM
                     EYTKDIIEKLHSKEIYVIEKMGLENIESLNDNTELFIRLFKEGDCKKNES"
     gene            complement(20862..21566)
                     /locus_tag="KQD82_001700"
     CDS             complement(20862..21566)
                     /locus_tag="KQD82_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380174.1"
                     /translation="MAWKFKYEKAEQEKLYAQTHQKTVEVAVEKKVPYEKCEKCDRTA
                     YRKAKEKCDSRKSQLEKKYKNMTIGYESILFLLAWYSITTTLFTAILSPVFLNDCIVF
                     FGALGKGMGSLFREFVTGADSFGQLSSGIPNSILSGIVYWLIAGTVMAILFVITGWLI
                     IGIGYQVGKIYRKYCWDIISIVVAITSTAVVIYFGEWIKNANPINLIVLLLLSHIIYI
                     GIRCYVKDWMEERGYY"
     gene            complement(21550..23271)
                     /locus_tag="KQD82_001701"
     CDS             complement(21550..23271)
                     /locus_tag="KQD82_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBG4380175.1"
                     /translation="MAIFHYTVKIVGRSKGKSIISASAYLNGDVMKNEETGRISYYTS
                     KKEVVYTSLMMCENAPQEWQNVPAENIRRFQKSVRYKRADNQNAALEKFKLTFQKQRL
                     WNEVLRIEKSSDAQLGRSFEFSLPKEWSRQEQIDYTTEYIQKTFVDKGMCADWSIHDK
                     GDGNPHVHLLVTMRPFNSDHSWGNKEIKDWEFVRDENGNIVVDESHPDWWQDKKDPDR
                     HGIRIPVLDENGVQKVGTRNRKQWKRVLTDATGWNNPKNCELWRSEWANVCNAHLKTE
                     NHIDHRSYARQGKLEIPTIHEGADARKIDEKFQNGQTSSTSWKVEENQIIKRQNALLD
                     KIQISFGKVSSALTQWKERLRDIRRKPGSHSHDGDNDKPDRGTAEYYGRDGTGIAGTG
                     QAAPVFSGAEPEFKKLKQRIIQDAKSFARYRRTALTDRATENQNRTVGKRESAMAGIN
                     AEAEQREQLIAETEQRIADLKQQIEKAREIDERMQKLRERRAGGRTSADDRTDAGRTG
                     SERPDNPGTKQAAKRIADLEREIEQRKQSREYRSIADKIKANRGTIDQRDRQQEKSRR
                     RSRGMEI"
     gene            complement(23600..23914)
                     /locus_tag="KQD82_001702"
     CDS             complement(23600..23914)
                     /locus_tag="KQD82_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBG4380176.1"
                     /translation="MARSTKSYEERMLQLEKKEQESLEKAKQYAAQKRELKKRQKDVE
                     TKKRTHRLCQIGGAVESVLGSAIEEDDIPKLIGFLKRQEANGKFFSKAMQKEPVANTE
                     EV"
     gene            complement(24059..24289)
                     /locus_tag="KQD82_001703"
     CDS             complement(24059..24289)
                     /locus_tag="KQD82_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380177.1"
                     /translation="MTQRKIALSIEEAADYTGIGRNTLRKLVEWKKLPVLKVGRKVLI
                     KTDMLELFMEANEGRDLRDKGNVKAVTRNGST"
     gene            24465..25094
                     /locus_tag="KQD82_001704"
     CDS             24465..25094
                     /locus_tag="KQD82_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380178.1"
                     /translation="MKDKELRKLIGSRAKQRRLELNLTQPYVAEKMGVTASTILRYEN
                     GSIDNTKKMVLEGLSEALHVSIEWLKGETDEYETDITDKKELQIRDVMGDILKQLPLD
                     LNKTEDAFSKDLLLLMLKQYELFLDSFQFACKNYKGSTKDVDIAKVMGFESKDEYNEI
                     MFLREITHTVNAFNDMADVVRLYSKKPEAAEQRLANLLSEVMYDDSESV"
     gene            25255..26550
                     /locus_tag="KQD82_001705"
     CDS             25255..26550
                     /locus_tag="KQD82_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4380179.1"
                     /translation="MAKGSVRKKGKKWYYRFYVEDASGKMVQKEYAGTESKSETEKLL
                     RKALEDYEDKKFVAKADNLTVGELLDMWAEEELKTGTLSNGTVENYLGAIRCIKKHPI
                     ADRKLKTVTAEHLQGFLDLLTFGGEFPDGKVRKGYSKDYIHSFSAVLQQSFRFAVFPK
                     QLISFNPMQYIKLKRQAEEVDLFSDDEVEESTQPISHEDYERLIKYLEKKNPPAILPI
                     QIAYYAGLRIGETCGLTWQDINLEEQCLTIKRSIRYDGMKHKNIIGPTKRKKVRIVDF
                     GDTLTEILKATRKEQLKNRMQYGELYHRNYYKEVHVKNRVYYEYYHLDGTQEVPADYK
                     EISFVCLRPDGSLELPSTLGIVCRSVSKKLEGFEDFHFHQLRHTYTSNLLSNGAAPKD
                     VQELLGHSDVSTTMNIYAHSTRKAKRDSARLLDKVASNA"
     gene            complement(26751..27578)
                     /locus_tag="KQD82_001706"
     CDS             complement(26751..27578)
                     /locus_tag="KQD82_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG4380180.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            27960..28280
                     /gene="sugE"
                     /locus_tag="KQD82_001707"
     CDS             27960..28280
                     /gene="sugE"
                     /locus_tag="KQD82_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG4380181.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(28413..29093)
                     /locus_tag="KQD82_001708"
     CDS             complement(28413..29093)
                     /locus_tag="KQD82_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG4380182.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            29489..30541
                     /locus_tag="KQD82_001709"
     CDS             29489..30541
                     /locus_tag="KQD82_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG4380183.1"
                     /translation="MKSVRFYGIRDLRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGIWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            30588..31004
                     /locus_tag="KQD82_001710"
     CDS             30588..31004
                     /locus_tag="KQD82_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380184.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            31033..31506
                     /locus_tag="KQD82_001711"
     CDS             31033..31506
                     /locus_tag="KQD82_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380185.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIIIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            31553..32311
                     /locus_tag="KQD82_001712"
     CDS             31553..32311
                     /locus_tag="KQD82_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380186.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIAPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            32335..33174
                     /locus_tag="KQD82_001713"
     CDS             32335..33174
                     /locus_tag="KQD82_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG4380187.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKDELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT        11224 a         4999 c         5558 g        11584 t
ORIGIN      
        1 gtatatgaat ttaattgata attaatatga aaattatgtg aattttttag aatgtacagt
       61 aaatatatta aggattatag atttttttgt catttaatat ggataatgtt atcttagatg
      121 ctgaactact attagaataa tctttgcttt gaatatttgt agcaaagaaa taagtatttt
      181 catttttttc cacataacct ataaaccaac catttacatc ttttccattt atattagcag
      241 tgcctgtttt accaaaaagt gataccccat ccttttttga tattaacaat gaatctttta
      301 ctgtttctat atttttatct tcaaatttaa acttatttgt ataaaaactt tcaagtaatt
      361 ctacctgttc aataggtgat atttttaatg aagattctag ccaaaagttt gagataccac
      421 ctgaaacatc ataattgcca tatccaattt gcctaagata agtttgtatg ttttttagcc
      481 ctgttttttt atctagagtc tgaaagtacc agtttacgga gttttccata gcagaaaata
      541 agtcttggtc ttcattccaa gaactattag aatgatgctt tccatcccaa gggatatgtg
      601 aattatttct tgttattatc ccattttcca atcccaataa ggcactataa atcttataag
      661 tagaatttgg agaaattctt gttttacttt tttctttatt gtaaatatga tattgcttgg
      721 attttaaatc atacataaca aaacttccat caaaaccttt aaaatactga cttaagtcct
      781 cgtacacagt ttgttcattt gtaggtctat atttttcgtt gatatctgcc ataatagata
      841 gtttgggaat atttccaaat acaattaaag ctatcaatgt aaaaattaaa atgcttttga
      901 attttaacgt atttgattca ttatgaaaat ccattatctt caatagtcgc ttttttattt
      961 gtttttttga cccaccaata tctgatgtaa gtgtaagatt tgatggtctt gaaatattat
     1021 ccacaaagtt tattagagtt tgtccatatt ctatatgact atctttatca agctttttta
     1081 aaactgaaat atcacatgca atttcacggt ctatacgcat ttctttaaat gcataccata
     1141 caagaggatt aaaccaatat aatatttgaa ggaagcacat tatataattt atcagtatat
     1201 ctttattttt aaagtgttgt aattcatgaa gtaagatata ttttatatct tttaaagata
     1261 atttagatat agacttatct ggtaatataa tgtgtggttt aaagtatcca aatgtaatag
     1321 gtgtatttat aataggtgat ttgcatagta taaaatcctt atttatacca atatcttttt
     1381 tacacttttc aaatataaat actatctcct catctcttaa aatttgtatt gattttttca
     1441 tattttttat tttaatgttg caatgaatag taatagctat catgattaaa attccaatta
     1501 tccatgatat tagtaataaa atatttaaac tatcaaaatt aacttggttt agggatactg
     1561 aaaaatcgtt taatgcattt gagttattaa ttatagaatt tggactattt tttatcatag
     1621 aagtcctatt agtactgctt ccaagttgat taaaagtata taaatagtta aatacatttc
     1681 caagattaat atatttaaga gggacaaatg gaattattaa cattataagc aatataaatc
     1741 caatattgta ttggtacttt gcagaaatat gttttttaaa tacttttttg gcaagtaaaa
     1801 ttacagcaat taatattgat aaggttaatg tactgagtaa aaatcgtata aagaatgggg
     1861 catacatatt tatttatccc ccttagtcgt ccttttattt aagatttctc tcagttcctc
     1921 aatatcatct tcagatagtt tatcattttc taaaaagttt agtaccatag aattaagagt
     1981 accattataa aaacgattta aaaaagaact actttcttta gctacgtatt cttcttcttt
     2041 aacaagtgga gtataaacga atactctgct atttttttca tatgttaaag ctcctttttt
     2101 aactaatctc aataacattg tttgaattgt tttaggactc catgtacttg tttctaccaa
     2161 cttttcaatt acttcagtag tgctgatagg agtatatttc caaataattt tcataacttg
     2221 atattctgct tctgaaatat gtggtaactt tttcatacta tacctccttc tacttacatt
     2281 tgtaatacat aaagtatatt atcattaatt tagtttgtca attattaaaa ataattcatt
     2341 tatagttgat taaataagca catgtagtta aataaaactt ttatttaagc tgtgtaagta
     2401 tagctttaaa taaatggttt atattgacag ctaaaaatat agttgatata cttattgttg
     2461 atataacaac taaaaaggag atatagaaat gataattaca gttataaatg gaagccctag
     2521 aaaaaatgga gctacatcaa aagttttaac ttacttgtat aaagatatag aaagattaat
     2581 accagatgta aaaattaatt attttgattt aagtaaggta aatccatcat attgtattgg
     2641 ttgtttaaat tgttataaaa tgggcaagtg tatcaatcaa aatgataaag tagagtatat
     2701 tcatgatata attactaaga gtgatggtgt tatatttggt agcccaactt atggaagtag
     2761 tgtaactgga cttttcaagg tgtttacaga tagagcacat atgatgcttg aaagattatt
     2821 gtatcgtaag ccatgtatag cagttactac atatgaaaat gcacgtggtt caaaagcaat
     2881 ttcttttata aaatccatgg tcttagattc gggaggttat gtttgtggta gtttatctat
     2941 aaaaactgga tttaatcaaa atcctataac tgaaaaagta gaatcaaaga tacaaaaggt
     3001 atctaaaaaa tttatttatt gcatagagga aaagaaaaat ccacctgtgc tttctcaaat
     3061 ctataatttt attgctataa atgctgtttt aaagccaatg gcttttaaag atattgagca
     3121 atataaggga ataattgata gatgggagga gcaagggatt atttaaatgg aggtaaaatg
     3181 aaagattttg agaaactata tgaagctaca actaagttag taagtacttc gttaaaagta
     3241 aaagatgatt taaaaaagat gtttaaacaa aatggatatg atataacaac tgaccattat
     3301 gcgttattaa ggtttctatg ggagcaagat ggaatttctc agatagattt atgtgaaaaa
     3361 tcatgtaagg ataaatctaa tacgacaaga atcttagatg ttatgaaaaa taagggatta
     3421 attgtccgta aagtagatgt taaagataga cgtaagtttc aaatattcct aacagattta
     3481 ggtagagaat tagaagaacc attaaatgaa attgcaagta tatatgcaac agagacattt
     3541 aaatctatct ctgatgaaga attgcctttg tttactgata ttttggatag aattggaaag
     3601 taaatataaa tcctatttta agagactttt taactattga tgatgtagaa atatagaaag
     3661 agggataaga atgaatagta agatttatta tttttctgga acaggaaatt cgctagtagt
     3721 ttcaaatatt ttaaaagaaa cgttaaatgg aagtaaagaa gttataccct tagctataca
     3781 tagaaaagaa aataatatag acataaatga agatatattg gtaattgttt ttcctgttta
     3841 ttttgctgat gttcctgata tagtaaagtc ttttgtagag aaattaaatt ttaatattaa
     3901 tactcatata tatgccattg ccacttgtaa tggggtagct ggacatagtt tatttactat
     3961 agataagtta cttaaaaaaa gggggaaaaa attatctgct ggttttttaa tcaatatgcc
     4021 tggaaatgct ttgataacac ctccagatgt tgaagaagaa agattgaaaa atcagactaa
     4081 tagggtagtt gagattgcaa aatgtatcaa taacttggag attggaaata tagaaggaaa
     4141 aaacaattta aagtcacata tagatagtgt tgtacttcgt ttagtaggta agaatatgca
     4201 aattgcaact actaaatttt ttgcagaaaa taaatgtaat ggatgtggga catgtaaaag
     4261 ggtttgtcct gtagagaata taagtatagt tgataaaaag ccaaagtggg gtaatgagtg
     4321 tgaaagatgc ctagcttgtt ttcactggtg tccaaaagag gctattaatg taaaaaaatc
     4381 ttttttaacg aataggaaga agtatcacca tccagatatt actgtaaaag atatattttt
     4441 agataaatag atatatttga cttgtattaa taattgtttt attaaataaa aacgttagag
     4501 atataaaaga aatataaagt aatcttgtaa agagtaatgt tacctagact aaatggaata
     4561 gagattaaaa agaacaatat aaataataaa ttatgttaat agtatatagt tacactaata
     4621 taaaataaaa gtgcctcata aatagaagtt tttgatgcac ttttatttta tattagtttt
     4681 tattattttg taatatcaac cgtttataat ctcgaacatt ttttttatat cttctgatgc
     4741 catttcaaat tttgattttt caatcagtat gtttccacca gtcataatat atgatggaaa
     4801 tactcttaaa ttatcataat aaagttcatt attacgaagt ttatatgttg aaatggaagg
     4861 gatgacattc ttttttatat gctttttagt aatcatatta aaagattttt ttgcaacatc
     4921 tcccattaac attactacct ctaggttttt aaataattta atttcttctt ctaaaatagg
     4981 catatgtaat ttaattgtat cacgagttat tgtatattct gatttaggaa gttttactgc
     5041 attagttata tagataccca tatctaggat atcatttatg ttagatactt ttattcctgc
     5101 actgtcaaat aaaggaatag tagtttttaa ataatcagca tctttatcta cactataaaa
     5161 atcgtcattg gtgttagttg ggacaacctc attaatcatt atagttttta ttttgtctgt
     5221 ttctattgtt atattaggaa ggtaggatat tattctgctg tcatcaaaaa gattgttctg
     5281 tttaataaat tcacttaact ttgattttat atttattttc atataacgag tctccttagt
     5341 tttaagcttt aaattactat aaaaagctag ttttaaagca cacaattttg aaaatatagt
     5401 agcatatttg aaagtataat aacatataaa acatgacaac agaatgtcat gttttataaa
     5461 taatctatcg caacttagaa tttattagag actctgcctg ttcataatcg tctttatgaa
     5521 cataaattaa gtactcataa gagaaatctt ccttttgacc taaattgcca agtgttattt
     5581 cattaaaaaa aggtttttta gagtaagcat tatttttcac tttatactta tatttaattt
     5641 tatttactga taatatattg catatctcgt taaatctatt taaagaatat cctcggaaaa
     5701 gctcagctct atttaaaatc ataaggaaaa cctcctttta aaagtaatat attgaaaagc
     5761 ataggagaaa tgtactataa gttgttataa ataaaaggta gcaaatttaa attgatttgt
     5821 aaagttttta agagtattat attagtgata aaataaataa tttttagaat ctaactaata
     5881 acaatttaaa ataagtaact tatgtaagaa ttgtaggaac tacggagata tcctgtagag
     5941 aattaataag acatatttaa gtatgcaaaa ttctattaag tagaaacctt gtaactttag
     6001 tcatgtgaga ttcataagaa tatttaagta ttgaaaggag agtttaattg aaaaaaatat
     6061 ctcaagaaaa aataagtaca catatccaag aaataccaca ggaaaaaatt gaacagtgga
     6121 aatctttata tgaaaaatat aaaagtattt taaaagtaaa ttctaagtct gttttagaaa
     6181 taatagagtt tttaaagcga aaatatgtaa tagtagaaga aacttcagag gaatttaaac
     6241 atgtagttat caataatatt aagttaaaca agcctttttc acaaaagatt tccagtgata
     6301 aagaattaaa acctattgta tttacaattc caaatgaagg aaatgcaaaa aaattgtacg
     6361 atactcaaga ggaaatttat agtggctgta agattattgt aggtatggaa tttgaaacag
     6421 gttatgtttt tgtagaaggt agtgatgagc ttaatgatga aataacttct tttaaaggat
     6481 tagataaata tgatataaat aattattatt tagtagcaaa ctatattaga tgtctaaaga
     6541 aatataaatt aatagattag tgtttgatta ataaattagt gtttgattta aggattatag
     6601 agtaaatttg aagttagtgt gataatgaaa ctaagcttaa atctaatata actgtaaaac
     6661 ataaacttaa atagagttgc caaaataaaa ttggtaactc tattttttat tttaaatttt
     6721 attaaaatta gtaggtgtta tagaattgtt attttttcat gttagtattc tcttagtaac
     6781 agatttaagg tattacaaga agggagaaat taatatgaaa gatttaattc ttgaaacaaa
     6841 aaacttatca aaaagatatg gagaacaaat ggcagttaac aatgtttctc tacaaattga
     6901 acgaaatagt atatatggac ttttaggacc aaatggagca ggaaagtcca caacacttaa
     6961 aatgcttgtt ggtcttcttc gcccaacaaa tggtcgtatt atttttgata gaaagccttg
     7021 gaaacgagaa tctcttgcaa aaataggttc tttaatagaa tcccctgcat tgtatggaaa
     7081 ccttacagca gaggaaaatt tgttagtaca tacaaaaata ttgggaatac ctaaagataa
     7141 aatttatgaa gtgttggaaa tagtaaattt gaaaaataca ggtaaaaaga gagcttcaca
     7201 attttctatg ggtatgaaac aaaggttagg aatagctatt gcacttttga gtgacccaga
     7261 gttgttaata ctagatgaac caaccaatgg tcttgacccc tttggcattc aagaattgcg
     7321 tgaattgatt gcttcttttc ccaaaaaagg tataactgta attctttcta gccatattct
     7381 atcagaggtt gctcaagttg tagataatat aggaattata aatggaggac gactattatt
     7441 tcaaggaatg cctgacccta atgaaaatct agaagaattt tttacagatg ttattcttaa
     7501 aggaggacaa aacagatgaa tgctttacaa tcagaacttt taaagtataa aagaacattt
     7561 atgggtaaac ttattgtttt tttcccagtg tcttttgcag catatgcatt tataatgcaa
     7621 tcaacactca tgcaaaatcc attatctcaa acaacttctt gggcgtggca aaatctttta
     7681 gcattaattt ttaactggtg gtcattttta tttctgccta ttggatttgc attgttcgct
     7741 actttggtag ctttccagga aaaaaaagct ggaaattatc gtgctttacg tacacacaat
     7801 gtgtctccta tgacactttg gattaataaa gttatagcta tggcagtcta cagttttatt
     7861 tcaacgctag ttttaatagt cgtaacaatt ataactggtt taatattgaa agctggtcca
     7921 gttccttttg gacagattat aggggcaagt attgtatgct gggttgtttc tcttgctata
     7981 cttccacttc agctatggct agctacatgg aaaggtatgt tcctaagtat gggagttgga
     8041 gctttaggta tgatatttgg tgttcttgct gcgacaaaac cattttggat tgcagtacct
     8101 tggagttggg cagttagaat ggtatgccca atcatcaaca tacatcctaa cggtactata
     8161 ttagaagctg gagacccact tttgaatact tcagtgattc ctattgggat tgttgtttct
     8221 ttggttgtgt ttatagtatt aacagcactt actgcagctt ggttcaatag gagggatgac
     8281 aaatgataaa actattatca tcagaatggc ttcgagcaaa gaggacagct gtccagtggc
     8341 ttactctttg tatgcctatt attttttcat tatgtgtggt tttttatctc acaactaaat
     8401 ctggaagtac acaagaattt gcttttgaag gattttttac agtatggact gtattcatca
     8461 ttcctattgg agtaggtatt ttagcaggat ttatagtgca ggaggaagag cttgctggaa
     8521 attttaatgg ttttttatgt gtaggtattt ctcgtgtcag attatatctg ggaaaatttc
     8581 tttttcttct gttttgccta actatatgca catttattgc tacgttgata ctttgcatag
     8641 gaatgagtat tgcagtgcca tctggagcaa caatatggtt attcctgtca gcagcaggac
     8701 ttgtggtgat tgggacactt ccactattag ctattcatct ttgggtgagt tttgcatggg
     8761 gtatgggtgc atctattggt attagtattg gtggaatttt gatggcagca atacttggat
     8821 taactagcgt tggtgaaaaa gtatgggcat ttgttccatg gacatggcca gtaaaactag
     8881 gtatgcttcc aggaatttat tttattaaag aagctggaac tatttcaact gaggcatttt
     8941 actctaaagt gatgcaaaca gctagtatag agcttatagt agtagcaata ggtttgatta
     9001 tattcttgat aggaggcgtt atttggttta aaatgtggga gggaaggaaa agttatgagt
     9061 agacgaatat tgttttaata ctgaccttcc cataagagaa aggtggtgtt atattgagta
     9121 aaatattgat tattgatgat gaaatggacc tagtaatgct acttgaggat gaattgaagg
     9181 caaaaggaca tgaagtgttg atagcttatg atggtcaggc aggtatagag ttatcaaaac
     9241 aggagcctga ccttattata ctggatatta tgatgcctaa aatgaatgga tttgaggtat
     9301 gtcaggcaat tagagatgat gttttatgcc caattatatt tttaagtgca aagcaatcag
     9361 aaaccgacaa aatcaaaggg ttgacctttg gaggggatga ttatgtaaca aagccatttg
     9421 gactgcgtga acttatggca aggattgaag ctaatctgcg tagggaaaag cgttcacagt
     9481 atatcaatga agagaacaag agatctactt tgtattttgg gaagttatgc ttatatatga
     9541 aggaacgtgc aattaaaatt gatggtaaga atattgattt aacaaaaata gagtatgata
     9601 ttgttgaatt actagcactc catgcaggac aagttttctc aagagaacaa atctatgaaa
     9661 aagtatgggg gtgtgattct gaaggtgatt cctcaactgt tgttgaacga gtaaaaaaaa
     9721 tacgagctaa gttttctgct gtgacaccag aaaaagaata tatttccaca gtttggggta
     9781 ttgggtataa gtggaataaa atgtaaagag agagaggtta ttaaatgaaa aatcaatccc
     9841 ttattaccca atttagacat acttttattt ttattataat agccagtata gttgccacag
     9901 tgattactta tgtatttgct ctctatttgt atattcattc cttaaataag gatatctatc
     9961 caccaaacta ttatgagcga caagttccta gaattgaaaa atacatcaat gaaaaaaaca
    10021 tagctttgtt atcacaatcg aatgaagaag gtttaaaaag aacgattcgt ggggatgata
    10081 tgctatatca agttgtggat aataatggca atattttata tggtactaac cccaaaaaac
    10141 tatttaaaac aaaagaagaa ttatttaata ataatatcaa taaaactgtt cgtaaaggtg
    10201 gttatatcca tactgttcca atcaaagggg ataatggaaa gattgagggt gctgttattc
    10261 tattctacca agtgaaaatc acctttgcaa atattagagg gagatttgta tttgcagtta
    10321 ttataatggc tctgttttct ccatttctat atatcgtggg ttttactaga tggctttcta
    10381 agcgctttgt aaagaatatc aatcaaccat tacatctatt gattgatgcg tccaagaaga
    10441 taaaagaaaa ggaccttgat tttgaaatcg actattattc agataatgaa ctcggtaaac
    10501 tttgtagtgc cttttctgaa atgaaagacg aattaaaagg ctctttatct gcacagtgga
    10561 agatggagca ggaacgtgtt gaaatggtgg aagctctagc acatgattta aaatctcctc
    10621 tttctattat tttgggatat acagatgcac ttattggtaa taatacagat gacaatgaaa
    10681 aacttcatag gtatcttaca gtgataagag aaaatacaga aaaaagtgct gctcttgtcc
    10741 aaaagatgca atatacgtct gacttagaaa agtcaaatat acaattaaat cttgttccta
    10801 taaatttacc tgaatttttg aggcaaaagg tgcaagacta tgaattacag gctcatcaaa
    10861 aggaagttga acttatattg aaaatgcagg ggaatataca atccccaatt caaatcgacg
    10921 tggataggtt gacacgaatt tttgataata tcatatccaa cagtctgcaa tacactccat
    10981 caggtggtaa tattagtatt actgtaaagg atgaaaaaaa ttgtatttct tatgagattt
    11041 gtgattctgg tagagggttt agctctaaag atttaaaaaa ggctcttgat aaattttatc
    11101 gaggagatga agctaggcag acaaaaggtg gacattctgg tcttgggctt tacattgtca
    11161 aacaattagt ggaacagctt ggaggttctg ttaaaataga aaattcaaaa tcaggtggag
    11221 catgtgtgaa attttggcat agcatttaac tatatatttt aaattaaagg ggagtgtctc
    11281 aaagcgaaat ttttaattcg tgaggcactc tttttttgtg aagttaaata tattttcatt
    11341 atttcgacaa tgaaaaaagg cttatctcta ttttgagaca ggcctttttt aaagcttata
    11401 ttatagttaa aaagatttat tagttagatt tgaaagaaaa tagagacagt cattgataat
    11461 ttaatttata ttattatctg taaaatagtt ataaattgat atctatacag tcaattatat
    11521 tcatcttttt aacttttctt aacggaataa gagtagctat aatacatgca agtacagaaa
    11581 taatagtagc ttgtaaaatt gaagtaattg gtatattagt gaaatcaagc ttttctatag
    11641 ttgccatgtt tataataata gcactggcat acccagcaat tgaaccaaat atagcagcaa
    11701 aaattccata ataaacacct tcccataaaa acattttata taagctacta ttactcatac
    11761 caatagctct tttaactcca atctcattag tcctagtatt tatatttgta tgagttgtgt
    11821 taatgatgtt taaagtacca atcaatccca caaataatat aactgcccat gctagaaatt
    11881 ctatttgttt aaaactctct ttaagttgtt tatctgattc aatatatgat atccaatgag
    11941 agccaggatt attttcacaa atctgttcga tagacaatcc tatttgttct aattttgatt
    12001 tatctttagc ataaatattt atctggttga atttgttttt atttgtcaag gtgttataaa
    12061 cagaatcata tacaataaca tcaacaccat ttatccagcc attaccttgt aaaaagaaca
    12121 ttttatctac tgtactatat atatctagtt gttttttgtt gattgtgata ctatctttag
    12181 gttttaaatt tgtatgtcta gtttttacac ctggtgtaga aatagcaatc ggatttttta
    12241 taaaacagat agaaccattt ttaaagtctt ctagtataga atctgtaata gaaggcatta
    12301 agtcttttaa agtttgctca tcaattccaa tgatttttaa catctcacca ctattttcaa
    12361 aatttatatc tgtatctatt tccccctgtt tgtattcaga gtactttata aaggaagtgc
    12421 gattgacgtt tttcatttta gttattttat ctagtgttga tttatcaaaa ccagatattt
    12481 catttgttaa ggaaaaatct ccttctttta aattacttac tgacctagat acatctaata
    12541 cattagagaa ctcactcagt gcaacgaata ctgttatact cataaataat gatagtatag
    12601 ttattatagt tcttccttta tttcttttta agtttagcca tgctagatgt ctttcaaagt
    12661 tttttattgt tttttctctt ttaatttttc ttttgatttt ggtggtacta ccaaccatag
    12721 caattttggg agagacatgt gaagcatata ttgctgatgg taatgctgaa ataaatgaaa
    12781 aaatcaaaga tacattagtg cttaatacta atgggaacat atatgctgtt gtatttttat
    12841 gtataagctc agtaatttct ttattatcat ttgctagaag tatatctgga tttaagatat
    12901 tagtgaccat tccagttatg cctttagaag atataattcc aattacagct ccaataggta
    12961 tggcaacagt acataaaatt aagatttgta aaataactat cttatatatc tggtttggtt
    13021 cagctcctat tgctcttaaa catccatact ctttaatttt ctttgtaaca gagattttta
    13081 aaatattata gattacaagt ccagaggcca gtaaaaccaa taaggcaaca aataaagata
    13141 ccaatataat catagatata ccatcactat tagaactatt ttcatctttt tcaaactgta
    13201 tcttaagagc atttaagtaa atccaattat attgaattga attgtgagaa agatttattt
    13261 ttttagctaa ttgatttact atttcttgaa acttttcttg ctgttttatt ttaaaatcca
    13321 aagaggataa aatatatctc ttaggtaata gattttctga tgtaccttta cctacaatac
    13381 catttactat accacttaca tatcctgtat aatcatcctc taaaatgcca ctcaatttaa
    13441 agttagctgt gtaattatat ggtggtatgg tatcatttaa aagtgaaata tctaaattca
    13501 taggtatagt cactcctagt cttggtttta tacccataag ctttagtgtg ttttcatcta
    13561 aggctatttc gtttttatct ttaggcaaat gtccagattt taatttcatt aatttggggt
    13621 agttagaaag tcctgtttta tcatactctt ttacaagtac agagatacca gaatccttta
    13681 ttttactact gccaatagtt attgaagtac cagcttgata aactctatca tctttcttta
    13741 tgtcatcaac ttgattttta gtgagttggt gaaaggatac atgtctatca ccattaaaag
    13801 aacgggcttg ttcaattctt aaattttgta ggataccaat tgactgtcca accacagttg
    13861 tcataatagt tgatagtact attgctataa tgatgaatgt agtcataagt ttctggcttt
    13921 ttaattcttt tattgaaagt gaaaggtaat tcttcatttc tatttcacct ccataagaac
    13981 accatcaata attgaaattt ttctatcagc aagttttgca attctatcat catgagtaat
    14041 taccactaaa gtctttccaa actgttttga agtttttata agaagattca taacttcact
    14101 gccactttta ctgtccaagt ttcctgtagg ctcatcagca aatattatat ctggttttgc
    14161 aataagtgct ctagcaattg caactctttg ttgctgaccg cctgaaagtt catggggaag
    14221 atgattgagt ctatctgcaa ttccaagtaa tgatgaaatt tcatttaaat agcctttatc
    14281 aggttgtttt ttatctagaa gtacaggcat tgaaatattt tcagatgctg taagaacagg
    14341 taaaagatta aattgttgaa agacgaatcc aaaggatttt cttctaagtg cagatagttc
    14401 tttttcagtg tgtttatata tatctttatc atcaagtaac acttgtccag aggtaggatt
    14461 atctaatcca cttaaaatat gtaaaagtgt gctttttcca cttccagaag gtccaattac
    14521 agaaacaaat tcacctgagt ttatttctaa atttacatta ttaagtgcga taactttatt
    14581 gttattagaa ccatatattt ttgataattg ttttgctttt ataatcataa aaaacactct
    14641 ccttttctat ataagaagag tgtaagtttt aaaactcaat tttttcccaa cttttagctt
    14701 aaatctaaaa agtcttcact atttgcggat aattcgcagt gctgataagc agtttcagta
    14761 tcatttttaa tttaaaatgt aatttatgca ctaaaggctt tacaatacaa ctctattgct
    14821 ttaaaactat tttacacata aacttgtgga taatttttta tgttttactt cctcattatt
    14881 atataaatta tttttgtcac aaattctaag ttgtcacata aatatacttt ttatatcacg
    14941 atttaattct tttgatttta tcataattgc tatttcttca aaagaagaaa gaaaagccac
    15001 gatacatatc ataattcctg caacatcaag accaaagcaa atatagagta atggaaacgc
    15061 aaagatagaa attccagtga ttttattcat caaagtatga agcgatgaaa atgtgcgata
    15121 cttaaaaaaa ccaatactgt aactaatcaa ccgtaacaat gctattgcaa caatacacca
    15181 ccatagatac atgggaagag caatattttt tccgacagta accaaaattg taaccgcaaa
    15241 aatgaaatca gcaatactat ctaactttgc acctattcca ctttgttgtc tcaactttcg
    15301 ggcaataaga ccatcaagta tatcactaat tccacaccaa aaataacata tccagaaagg
    15361 cagtgaacag aatgtagaac taagcattag caatgcaaac acaatacgag agaatgtaat
    15421 attattagca aaacttttaa tataatcacc tccaaaattc taatttcata ctattctaca
    15481 tatttaaata tatatttcta ttattgtttc aaatgccttt tgccaaaata ttataattga
    15541 agcaactctt acaacttcta caaaatagta tatatattgg ttgtaaattg aaatacattt
    15601 tataaattct aaactaacaa attttatata atagcagtga aagcttacaa aatcatttta
    15661 atttcttgtt gagaatttag cagtaatttt agcactgtta gtagaattat tttcaataga
    15721 aatagagcca ccatgttttt cacataatat ttttgagata taaagaccca taccaaagtg
    15781 tgcattgctg tcatttaaag tcttatctct atagaaaggt cttaaagcta attttagaga
    15841 ttccttacta aatcctatac catcatcttc aattacaaag gtaaggagtt cttgttcaag
    15901 atatataagt atggatacat tatttctagc atatctaaaa gcattcgata ttatattttc
    15961 gacgactcta agaatgatat tttcatcaaa aaatagattt atattattta tttgattttt
    16021 aagtgtaaaa gattttcctt cttgtttagc aatgatagat atattttcat ctaagttttc
    16081 tataaacttg tttatattcg acatagattt tgaaacaact aaatcttcta atcgttgagc
    16141 attactcata ttttctacat agtattcaat tctgttaata tgctcagaca taagttgtgt
    16201 ggttgataaa atcttctcat cactaagctt acctgttggt atgtattttg ttaaatagtt
    16261 tgagtaacct tttagaactg ttaggggatt tcttaaatca tgagaaaaag cagcattaag
    16321 ttgttttcta tcattaatca tagtccacat ctttatgttg tttttattta gttcagacct
    16381 cattttttca aaagcattac atagattacc taattcatca ttattattat attctaagca
    16441 aaagtctaaa ttgttgttgg atatttccat tgcaccttta ttgagtattt ctatgggtgt
    16501 ttttaatttt accttgtaaa aaattatatc agctaataat aaaagtccta taaaaaatat
    16561 aagtggaagt acaagctgga gtatgttgat tatattaagt aatgttttat cataatcact
    16621 aaattggttc acattgtttt gaactatggt tataatacct gtatttatag gtgtttttaa
    16681 atacttatct tgtatagaat tatatagatt attaagtaaa atgtatgcga aagtagtaag
    16741 tactgatgct ataattagtg aagataaagt aagaacaaag aaagcttttt ttaatgacat
    16801 attatttatt ttatccattt ataaccaact ccccagactg tttctatata attttcaaga
    16861 gtatgttttg atagtttgat tcgtattttt ctaatgtgtt ccattataac atcactattt
    16921 ccattgctat tgtatcccca gactctatca tatatttttt ctttatcaaa tacttgtcca
    16981 ggattcatag agagaagctc tactatatca aattcctttt ttgaaagtga aatctgatta
    17041 ttatatatac aaattaacct tttttcgtaa tctattgtta aatcattata aaatttaatt
    17101 ttagtttgat ttgacttcct ttgttcacgt cttaaatggg cagatactct agctccaagt
    17161 tcgtctaaat caaaaggttt tacaatataa tcatcagcac cagcacgaaa accatttatc
    17221 ttgtcagtgt tttcaattct agctgtaaga aatagaatag ggcatgatac ataatctcta
    17281 attctattac aaacttcaat tccatctatt tcaggcatgt ttatatcaag tagaatgata
    17341 tctggatttt tggatatttt ttttaatgcg tcttttccac tttgagcagt gtaaacctca
    17401 taaccattca tttcaaagta atcttttaag agtgttatta taccttcctc atcatctacg
    17461 acgagtattt tattgttcat aagaatgctc ccttctaaat taagtttgta tagtatgtat
    17521 attttagtat aaaaatctca ataaattatc aaatttttag ttatatttca aaacttaatc
    17581 attaaagtta gtatattttt atatattaaa taaatcaagg atgaaattat tagaaatttt
    17641 aatagaatat atatttgata tttaatactt attggtatgc aaaaatatag ctgatgaaat
    17701 cttataattt aagttgaaaa tggataaata gaaaggaagg attttaatta aataaggtag
    17761 attaattaaa aagtatataa attataaaaa tgaattaatc tacattaatt agttgatata
    17821 tcaattatta ttgttttttt cagttgctta aaatatactt gatatatcaa ctaatgttgt
    17881 tttttaatta aaagaaattt taggaaagat aatatcaact aggagagata atatcaacat
    17941 tgaagaaggt agagcaagat ttctacataa ttgtgaacat tatctggcat gtgtgcattg
    18001 gtgttttaag caagtaattc aatagaaaga tgtgactaaa aatagaaaga aataccataa
    18061 tccaaatgta aagctagaag acataataag taaaaaagta aagatattta ataaaagaaa
    18121 taactaatag tgattaaatt agtaaatgct gataattagc ataatcatta gtaattatta
    18181 gtattattta attaaattaa ttttagcatt atttttatga aattaatttt aaatcaaatt
    18241 aatgagaacc ccataaattt ttttaacact taatttccga ttgagatttc acctgtttta
    18301 agaaatttaa taactgttat attttattat taaattcttc gcaacccctt gaaaacactg
    18361 gatttgtcgc aggtgagctt tcccttatta tttcccttgt gttatatcgt cccatcagtg
    18421 tttatgcact ctgataaggg caaatacaag ggcaagaaaa atctataaga cagtataaca
    18481 caaaataaaa gtgacatggt gaactgattg aaatgcttct taatttttgc tactactgat
    18541 tgattaaatg ataaggagat gggatacgat gagaacaata tttgcagaat acaatccaca
    18601 gtgcaacagc attgatgtat atactaatac gggctatata ctccgtattg attgtttgga
    18661 agcagaaaaa aatttaagaa ccacacctgg atctgattgt gctttgacat cgcttgcaat
    18721 cgatgagcca ttagaatatg caaggttata tcttgatggg aatttacaga tgtgggtaga
    18781 tgctgaagat tcattagagt tatgattatt ccaatcaaaa tctactagaa tttattaaaa
    18841 attagcttca taattttaaa aggacagccc tattgctatg aggctgtcct taaaaataca
    18901 atagataaac ctagattatt cttgctacta tctgattgtt aaattttctg tattccatat
    18961 tgcaaatctc ccttttcgca atattttatt agtatgaatg ccagaatcgc tgaaaaaaca
    19021 cgtgccaata ccatactcca ccatatcata atctgtccac caaataaagg tggtaaaaca
    19081 agcataaata tcagcatcaa tactggttca atgaatgtaa gaatgtatga caatccactt
    19141 ttttcagtag cataaaatcc agctgtcgct attctggtaa tcgcaacaaa tggtaatgac
    19201 actaaaaata taggtacaat tttagaaatc tcaatactta cttcataaga agctccgaaa
    19261 acccctccaa tatttgctct gtttacatac atgataattc caccacaaac agcaagaatt
    19321 attgcaaaaa tgtatgccat tctctgaact cctctcaggc tttcttgatc cttctcacca
    19381 taaaaacggc tcattaaagg ttgacttcca tcacctacgc cctgcaatat gagatatatg
    19441 atacagataa tataggatac acatgcataa gttgcaatag ccttttctcc cccataagaa
    19501 gcggagaatc ggtttataaa caccaatgaa atattaggag tcaaggctag cccaaatggt
    19561 gcaagaccta ctctgaatat agcgccacac atttctttta cattttttaa ggatacatac
    19621 acataaaact ttttaatata taatgcgtat atgattgcaa ttgctgcagt cacaccctgt
    19681 ccggctattg tagctgtggc tgcacctttc atgcccattt catatatcca tacaaagata
    19741 aaatccagaa caatattggt tatgaatcca ccaaccatcg caaacattga ccagaaagag
    19801 tttccataat ttctaatgaa tggcatcatt ccagttccaa gaatctgcaa tattgctcca
    19861 agtgctataa ttttgatata atctgtcgca tttgttaata taattccatc tgctccaagc
    19921 aatcctaaaa ttttagacga aaaggagtaa ataattatgg tactgataac acttactatt
    19981 acaagaagcc accagcttgt agcaataaat tcttctgccc gttttccttt cttttctgct
    20041 gcattaattg agtaataaac agctcctccc ataccaattc ctgttccaag agcctgaatc
    20101 aaagcctcaa tcggatatgc aatattaata gccgaaagtc cagcatctcc tatggtattg
    20161 cctacaaaat atccatccac tatcgcatat attcctgata atgcaaatgc tataatagat
    20221 ggaataacat atttaaaaaa taatgattta tgattcattt ttcttacaat ctccttcctt
    20281 gaaaagtctg ataaacagtt ctgtattatc attaagactt tctatattct ctaatcccat
    20341 tttttcgatt acatatattt cttttgagtg cagtttttct atgatatctt tcgtatattc
    20401 cattcctgat tctgtgaggc atatcagctt atttctcttg tctgaatcat cagataccat
    20461 attgatgtat ccttttttct gaaaatcttt tagtatcgta tttaccgttt gcttaggtat
    20521 attccatttt tcacttatca ttttctgcgt gcatcttcca ttactttcat aaaatgaatg
    20581 caaagcaaaa actccatttg aaggcagtcc ctgatccttt gaccattcct catacattgc
    20641 tgtacattct ttccataaag cataatatcg ttgcaactgt attatcatat tattatcttt
    20701 tctcatcttt ttcctcctta aattagtacg aatccggact tttatattat agtccgagtt
    20761 cgtactaatt gtcaagattt atttatatac tttttcttta tataaacaat caatagtccc
    20821 ttactaagga caggaagatt atgaaatttc cctgtcctct tttaataata tcctcgttct
    20881 tccatccagt ctttcacata acatctgatt ccaatataaa taatatgcga cagtaatagc
    20941 aatacaataa gatttatcgg attagcgttt ttaatccatt ctccaaagta aattacaaca
    21001 gctgtactcg ttatcgctac cacgatactg atgatatccc aacaatattt ccggtagatt
    21061 tttcctacct gataaccaat cccgattatc agccacccgg ttattacgaa taaaattgcc
    21121 atgacagtac cggcaatcag ccagtacaca atcccggaaa gaatactgtt aggtattcca
    21181 ctgcttaact gtccgaaaga gtctgcacct gtcacaaact cccgaaataa acttcccatt
    21241 ccttttccta acgcaccgaa aaagacgata cagtcgttaa ggaaaaccgg cgaaagaatc
    21301 gcagtaaaca gtgttgttgt tatgctgtac catgccagta gaaacaagat actttcataa
    21361 ccaatcgtca tatttttata tttcttttct aactggcttt tacggctatc gcatttttct
    21421 tttgctttcc ggtaagcagt acggtcacat ttttcacatt tctcataagg cacttttttc
    21481 tcaacagcta cctctaccgt cttttggtgt gtctgtgcat aaagtttttc ctgctctgct
    21541 ttttcatact taaatttcca tgccacggct tctgcgtcgg ctcttttcct gctgtctgtc
    21601 tcgctggtca atcgttcctc tgtttgcctt aatcttgtcc gcaatgcttc gatattctcg
    21661 gctctgtttt cgctgttcaa tttctcgttc aaggtcagcg attcgcttag ctgcctgttt
    21721 agttcctgga ttgtctggtc tttctgatcc agttcgtcct gcatctgttc ggtcatcagc
    21781 agaagttctt ccacctgccc gacgttctct aagtttctgc attcgctcat cgatctccct
    21841 cgctttctct atctgctgtt taaggtcagc aattcgctgt tctgtttctg caataagctg
    21901 ttctcgttgt tcagcttctg cgttaattcc tgccattgct gattctcgtt ttccaactgt
    21961 tcgattctgg ttttctgttg ctcgatctgt gagagcagtt cttctgtatc tggcaaaaga
    22021 tttagcatcc tggataattc gctgtttaag ctttttaaac tctggttctg ctcctgaaaa
    22081 gacaggagct gcctgtcccg ttcctgcaat tcctgtacca tctctgccat aatattctgc
    22141 tgttcctcga tctggtttat cattgtctcc atcatgggaa tgacttcccg gctttcttct
    22201 aatgtcacgc aatcgctcct tccattgtgt taatgcactg gatacttttc caaaagaaat
    22261 ctgtatttta tccagcagtg cattttgtct ttttatgatc tggttttcct ctactttcca
    22321 tgaagtagag gatgtctgtc cgttttgaaa tttctcgtca atttttctgg catctgcgcc
    22381 ctcatggatc gtcggtatct ctaattttcc ctgccttgca taagaacggt ggtcaatgtg
    22441 attttccgtt ttcaaatgtg cattacagac gtttgcccat tcgctccgcc atagctcaca
    22501 atttttcgga ttgttccacc cggtagcatc ggttaataca cgtttccact gtttgcggtt
    22561 ccttgtcccg actttctgga ctccattttc atcaagtacg gggattcgga ttccatgacg
    22621 gtcagggtcc tttttatcct gccaccagtc cggatgggat tcatccacca caatatttcc
    22681 attttcatct ctgacaaact cccaatcctt gatttctttg ttaccccatg agtggtctga
    22741 attgaatggt cgcatcgtca ccagtaaatg cacatgggga ttaccatctc ctttatcgtg
    22801 gatactccaa tccgcacaca ttcccttatc tacaaaggtc ttttgaatat attcagttgt
    22861 ataatcaatc tgttcctgtc ggctccattc tttcggaagt gagaactcaa atgacctgcc
    22921 gagttgtgca tctgaacttt tttctatcct caagacttca ttccataaac gctgcttctg
    22981 aaaagtaagt ttgaattttt ccagtgcagc attttggtta tctgcccttt tatatcggac
    23041 agatttctgg aaccgtctga tattttcagc aggtacattc tgccattcct gtggtgcatt
    23101 ttcacacatc atcaggctgg tataaacgac ttcttttttt gaagtatagt agctgatcct
    23161 gcctgtttct tcatttttca tcacatcgcc attgagataa gcagacgccg atatgatgga
    23221 ttttcctttg ctgcgtccaa caatctttac tgtataatga aaaatcgcca tgctccgttt
    23281 cccccattct gccgtatccg gctttgattt ccggcaggta acgtttttcc taaaagaaaa
    23341 tggtatctgc cggagcaccc tctgcgtaag agtgccctgt gcataacagc ctgcatggaa
    23401 tgtgcccctc tggcgaagag tgcctcctgc ggcatgagca ggtctggctg aaagccccgc
    23461 cgacggaacg agcccggcaa gcgtaagcgc agaccggttg ccacctgtgg caaacttata
    23521 cggacgacct ctggttgtcc ataagtgcgc ccttcataaa aaagcaatga agggaatgga
    23581 aactccactc cctccggcat tacacttcct ccgtatttgc aactggctct ttctgcattg
    23641 cctttgagaa gaatttccca tttgcttcct gcctttttaa gaagccgatc agctttggga
    23701 tatcatcttc ctcaatcgca gaacccagta ccgattccac tgcaccgcca atctggcaga
    23761 gcctgtgtgt ccgttttttg gtttccacat ctttctgacg tttcttcagt tcccgtttct
    23821 gtgctgcata ctgttttgct ttctcaaggc tttcctgttc tttcttttcc aactggagca
    23881 tacgctcctc ataactcttt gttgatcttg ccataaatat catcgtcctt tctttttttc
    23941 caaacataag tttcctgttg tctgtcagac gaagtacatg gtataatctg cttgcgtgta
    24001 ggtatatcat gactccggtc tgatagaacc tttggcggtt tcttcgggaa ccgctttttt
    24061 aagttgaacc gtttcttgtg actgctttca catttccctt atccctcaag tccctgccct
    24121 cgttggcttc cataaaaagt tccagcatat ccgttttgat caggactttt ctcccaacct
    24181 tcaataccgg aagtttcttc cactctacca actttcgcag ggtgttcctg ccaattcccg
    24241 tataatcagc agcttcctca atagataagg caatctttct ttgcgtcata tgaccacctc
    24301 ctttcgaatt gcattatgca atttcatttg tgctgtcagt atagcgtgtt tatattgctt
    24361 tgtcaagcat tatgaaattt tgctgctttt cttaaaattg catattgcaa tatattttgt
    24421 tgcgtggtat agtaatgaca taccgaaaaa ggaggcacat cagcatgaaa gataaagaac
    24481 tacgcaagct gatcggcagc agggcaaaac agcgccgtct ggaattaaat ctgacacagc
    24541 cttatgtcgc agagaagatg ggagttaccg cttccacaat cctgcgttat gagaatggtt
    24601 cgattgacaa taccaaaaag atggtactgg aaggtctttc ggaagcactt catgtatcga
    24661 ttgaatggct caaaggggaa accgatgaat atgaaactga tattacggat aagaaagaat
    24721 tgcagattcg tgatgtgatg ggcgatattc ttaaacagtt acctcttgat cttaataaaa
    24781 cagaagacgc tttttctaaa gacttactgc tgttaatgtt aaagcaatat gaactgtttc
    24841 tggattcgtt tcagttcgcc tgtaaaaatt acaaaggcag tactaaagat gtggacattg
    24901 ctaaagtaat gggatttgaa tcgaaagatg aatataatga gattatgttt ctcagggaga
    24961 tcacacatac tgtcaacgca tttaacgata tggcagatgt tgtaaggctc tattcaaaga
    25021 aaccggaagc ggcagaacaa aggcttgcaa atcttttatc cgaagttatg tatgacgatt
    25081 ctgaatcggt atagtaaggc aaccggagtt gtgatacaat gagcacaagc gagtcaggct
    25141 gtttacagat ttgacgagcg gcgaattggc ttatggactt gtccataaga tgcctacacg
    25201 caagaagcat ttcaacagtt ccgattgtct gatatcgaaa ggagacatac gattatggca
    25261 aaaggatctg ttagaaaaaa aggaaagaaa tggtactatc gcttctatgt agaggacgca
    25321 agcggaaaaa tggttcagaa agaatatgcc ggaaccgaaa gtaaaagtga gacggaaaag
    25381 cttctccgca aggcactgga agactacgaa gataagaagt ttgttgcaaa ggcagataat
    25441 cttaccgtag gggaacttct ggacatgtgg gcagaagaag aactgaaaac tgggacactc
    25501 agcaacggta cagtggaaaa ttatcttggt gcaatccgat gtatcaagaa acacccgatt
    25561 gcagaccgaa agctgaaaac agttactgcc gaacatttac agggtttcct cgacctgctt
    25621 acatttggtg gtgagtttcc agatgggaaa gtaagaaaag gatacagcaa agattatatc
    25681 cattcttttt ccgcagtgtt acagcagtca ttccgttttg cagtatttcc aaaacagtta
    25741 atcagtttca atccgatgca atatatcaag ctgaaacgac aggcagagga agttgacctc
    25801 ttttctgatg atgaagtgga agaaagcaca cagccgattt cacacgagga ttatgagcga
    25861 cttatcaagt atctggaaaa gaaaaaccca cctgccattc tgccgattca gattgcgtat
    25921 tatgctggac tccgtatcgg ggaaacctgt ggtcttacat ggcaggacat taaccttgaa
    25981 gaacaatgcc tgaccataaa acgcagtatc cgctacgacg gcatgaaaca caaaaacatt
    26041 attggaccaa cgaaacggaa aaaagtaaga attgttgatt ttggagatac tctgactgaa
    26101 atactgaaag ctaccagaaa agagcagctc aaaaaccgga tgcagtatgg cgaactctat
    26161 caccgcaatt actacaaaga agtgcatgtg aaaaacaggg tctattatga atactatcat
    26221 cttgacggta cacaggaagt cccggcagat tacaaagaaa tctcctttgt ctgcctgcga
    26281 ccggatggct ccctggaact gccgagtaca ctcggcattg tatgcagatc agtgtcaaag
    26341 aaactggaag gatttgaaga tttccatttc caccagttac gtcacaccta caccagcaat
    26401 ctgctttcca atggggctgc accgaaagat gtacaggaac tgttaggaca ctctgatgtc
    26461 agtaccacaa tgaatattta cgctcactca acaagaaaag caaaacggga ttccgcaaga
    26521 cttcttgata aagtagcgag caatgcttaa atcaaaattg gttcactgaa ccaattattg
    26581 atatgcaatg cgacaaaaat tccccttatt tcccttgcgg attccgcata agggcaaaaa
    26641 taagggaaaa tacatatcat ttcactattt aatgggctga aaagcctgta aaataaggaa
    26701 agttcaggaa atctctccac aaattacgat ttatggggtt atgttctgtt ttatttcaaa
    26761 gtattaagtt catataaatc agttcttctg tgcttaagaa gtggtaactg ttgtcttatg
    26821 tcaggaatta aatctctatc aatgtcagca taaataatac attctttttc atcagcttga
    26881 gcaattattc ttccccaagg gtctgaaatt cttgaatttc caaaagctat ataagaagca
    26941 ttcatatttc tagcaggtgc agcaccaaca tagaatatct gattatctaa agcacgcatt
    27001 cttatagata actcccaatg ggcgggacca gtagtcatat taaatgcagc aggtaagata
    27061 acgatttcag cacctttaag agccattaaa cgtgataact cagggaaacg aatatcatag
    27121 caaatagcta taccaacttt tccatattcg gtatctatta cagtgacatc atttccagct
    27181 gtaagagtat ctgattcttt aaaactaact tttcctgtaa cttcaatgtc aaataagtgc
    27241 attttacgat gttttccaat tagagctcca tttttatcaa atacatagca agtattatag
    27301 atttttccat cttctaactc aggaatacta ccagaaacta gataaattcc atgtttctca
    27361 gctaatttac ttaatatcgt tgttgtttca cctggatatt cttcagcaaa tttaggaaaa
    27421 tatttatttt catatgggca gttaaacatt tctggcaaaa ctacaatttt agctccattg
    27481 atggcagctt ctgttaccat atctacagca tgattgatat tttcaatttt attttctgta
    27541 gtaatcatct gacatactgc aattttatag aaattcatag aaattactcc tttcaatatt
    27601 tttatgttat tataacatat taaataaaaa ttagatattc ttagattgac ctaaatgggt
    27661 atggagagaa gtgaataaga atattaaaag gtatataaaa gttattttta ttatagatgg
    27721 aacccatata ttgacttttt ttatatatgt tgatagtatt attatagata aaatagaata
    27781 attaatagag ttttataggg ttccgcaatt attaatgatt ggtctggtcc gagataaaac
    27841 ccacaacata gtttttgttg tgaacacgga aggacaaaaa cctgggagat attttataat
    27901 atttctcagg ttattttatt ggaaaaataa gagacatatt tgaaaagagg gatgaattta
    27961 tgaaatggtt atttttgttt gttgcaggtt tgtttgaagt gttttgggct tttcaactaa
    28021 aaaattcaca tggattttct aaattaactc caagtatact tactgttgtt ggaatgattg
    28081 taagttttta ttttttgtca ttagcactaa aacatcttcc acttggaact tcatatgcaa
    28141 tatggactgg aattggtacc gtcggtacag ctattgtagg catggctatt ttaaatgagc
    28201 ctattagtat atcaagaatt gcatgtatag gttttattgt ggtgggaatt gtagggttaa
    28261 agctattatc tactaattag agtatattaa tttaaataag ctatgaaatt aaaggcgaga
    28321 aaatgtttaa aaataatcaa catttaaaaa gtctttactt catagcttat cttatttata
    28381 agtttattat atttgtaaat tatatgtata tactatttat ctaataaaag agcagttaaa
    28441 tccatagggt caactattac accatcttta tagacacgca tatttccaga tgctatttca
    28501 tctattaaga ttacttcatc attgtagaat ccaaattcaa atttaatatc ataaagctca
    28561 agaccttttt cagctatagt atcacgaact atgttagaaa ttttttgagt catgcttttc
    28621 attgaatcaa attgagcttg agtcataatg tttaaagctt ctaaaccttc agatgttata
    28681 agagggtcac aacggtcatc atcttttaaa gttgcttcta cataacaatc tagtttagca
    28741 ccttcttcaa cacatgaacc ataacgacgt aagaaactac ctacagctct ataacgacaa
    28801 ataacttcta atccattacc aaaaactttt gctggtagaa cttccatagt tgcttcttct
    28861 aaattagcac ttacataatg agttttaatt ccagcattgt ttagaatttc aaagaactta
    28921 acagatgtct ctaaattagc tctaccaatt ccatctatac ttagacctac actattttcg
    28981 cctgggtcaa atactccatc tttaccagtt acatcgtctt taaatttaag taagtaattt
    29041 ccattatcta attcaaaaac atctttagtc tttccttgat acactttttt cataaacaac
    29101 ctccaaaaag ttaaattatt atattgaata aggcattagt gtcaaaaaag ttctgatatt
    29161 cgcatgaaaa tttatattga taccttatat ttaatattat atcacaaata gaagggaaat
    29221 ttccgaacaa ttttataaaa ataacaaaaa aagtacattt tataaaactt ataataagag
    29281 aactaatata aaatgttagt gtcgtttata ttgacacaca taagttttaa tataagaaac
    29341 ttttgacaca ctttgacaca catttaatga taattttgta aaatgcatga atacaatgct
    29401 tacatatatt ggcatgaaag ttgctttaga tatttaacat ataaacaata ggaatatatt
    29461 ctcaaataaa aagtaggggt ggttaatagt gaagtcagtt agattctatg gtataagaga
    29521 cttaagagtg gaggatgtgg atgtaccaaa aatattagaa aaagatgatg taataattaa
    29581 agtaaaagta gctggaatat gtggttctga tatatcaaaa tattcaaaga caggtccaca
    29641 tatggttgga gaaattttag ggcatgaatt ttctggagaa gtggcacagg ttggtaaaga
    29701 agtaaggagt tttaaaatag gagatagagt tgctgtttgt ccagctatgc catgttttga
    29761 atgtgatgaa tgtaaaaaag gcttgtattc aagatgtaat aatgtagcaa taataggaaa
    29821 taaagaactt ggaggttgtt ttgcagagta tacaaaggtg aaagagagaa atttaataaa
    29881 aataccagat gagataagtt atgagactgc cgctgcacta gaaccagtat gtatagcagg
    29941 acatggtctt tttaggtcag aagctaaagt tggagatact gttgtagtac ttggtacagg
    30001 tccaatagga ttattttcaa tacaatgggc aaaaatattt ggttctacaa aagtaatagc
    30061 tgtggatgta tttgatgaga agttagactt ggcaaaagag ttaggggcag atatttgtat
    30121 aaatgcaaaa gaaaagaata ttgtagaaga aataaaaagg ttaactgatg gagatggagc
    30181 agatattgtt attgaatcgg ctggtactcc actgacatgt ggtcaagtac tgttgctagc
    30241 taaaaaggga ggtacagtat tgtatgcagg tgtaccatat ggggatgtag ctttgactag
    30301 agagcaattt gaaaagatag taagaagtga attaactgta aagggaattt ggtttggaaa
    30361 ctcctttcca ttcccaggaa aagaatggag tgctggattg tatcatatgc aaaagggtga
    30421 tatgaatgtt gaaaaattag taacacatag aataaattta gaagaagtac cagcttattt
    30481 tgaaaaagta tacaaaaggg atattttctt tggtaagata atgattaata tagataatta
    30541 gattagagta ctaaataata atccatataa aaaaggttaa gaaaggtatg attcatatgg
    30601 caggaataat tatagccaca catggaaata tggctaaagc aatgttagaa agtgctgaat
    30661 taatagtagg caaacaggaa aatgtagaaa ctttaggatt aaaccatggt gacagcatag
    30721 atgagtttag tagaaagtta gaagatggaa ttgagaaata taaggatgaa ggggttttag
    30781 tattgcttga tttttatggc ggaactccat ttaatacatc tgctattata ataaataaat
    30841 ttgctaaggt atgtgagtta gaatgtttga caggtgtaaa tttacctatg cttttagagt
    30901 tatttttaaa cagagatact atgaagttaa aagacttgaa gaatttatgt gaggaagttg
    30961 ggatatcagg aatcaaagat gttaaaactg ttttaaagtt gtaaaataaa ttaaaaatct
    31021 ggaggaataa ttatgagtaa tatagcatta acaagaattg atgatagatt aatacatggg
    31081 caagttatta ctgcttggtg taagattaca tcagcaaaaa ggataataat cgtggatgat
    31141 ttagtagtaa aggacccatt tatagttcaa gttttacaaa tggcagctcc atcaactgtt
    31201 aaggttgaag tacatgatgt agaatcaggt gctgaagtct taaaatcaca taatggagat
    31261 gaaaatttaa tagtattggt caaataccct aaaactgtct taggtttagt aaatagcggg
    31321 gtagatttaa aggaattgaa tgttggaggt atgggagcag ggcttggtag aaaaagtttt
    31381 tataaaaata tatctgtgtc agatgaagaa aaagaaatat ttagagagct aatatcaaaa
    31441 gatgttaaat gctttataca aatagtacca gatgcaaaga aaattgatgt aggaacatta
    31501 ttataaagaa gactatcttg taaatcttta aaagaaaata gggaggaaga atatgcacat
    31561 ttcactaatt caagcagtat taatagcaat tttttattat ctttcttgga gtccgtggtt
    31621 gacttatgtt ggatttttta catggaatag acctctattg gctggatttg taacgggaat
    31681 tatattagga gaccctgttc aaggagcaat tataggagca ggtataaata tgatatatct
    31741 aggctttata tcagcagggg gagctcagat gggggaccca gcttttgcag gatatgtagg
    31801 tactgcttta gctatagcat ctaagctaga tgtaagtact gcaatggcaa tagctgtacc
    31861 attgggaact gttgcaactg tactttggat tggaaagatg acagtaaatt ctttttttgc
    31921 acattgggct gatagagagg ttcaaaaagg gaatgttgat agagtagcat ttataaatat
    31981 agcaccacca cagatactgt tatttgcaat gagttttata ccagcattat tagttgttta
    32041 ttttggtcca aatgcgatag atggtatgtt ggaagtaatg aatgataatg tgttacatgt
    32101 atttaatgta attggggcta tgttgccagc acttgggatt gctatgaatt taaaattgat
    32161 tggaaacaag tttactatgc cttttttcat attaggaata ctaatggctg tttattttaa
    32221 agtagatata atagtaatat ctgttattgg agtaatactt gctcttacaa ttacttcaat
    32281 taagtacgga aaagatagtg ctacatctta aatacatgtg agggggaaaa tattatgtca
    32341 gatataaaaa atgaagtatc taagaaaacg ttaagcaaga aagatgtaat aaaatcatgg
    32401 ttaagatggt ttttctttgc acaatctaat tacaattatg aaagattgca atcaacagct
    32461 ttttcacatt caatgttgcc agtacttaaa aaattatatc cagataagga tgagttaaaa
    32521 caagaagtgg agactcatct agcatttttt aacacagaac ctatatgtgg atgtgttata
    32581 catggaataa caatagctat ggaagaagaa aaagctaatg gagcagatga aataagtgga
    32641 gatggtatga atgctatcaa aacaggcttg atgggtccac tagcaggaat tggcgatact
    32701 ttaactcaag gagttataac tcctatagtg ttggctgttt gtattggttt aactgaggga
    32761 ggagcatcaa ttgcaggacc tatattattt gtaatagctc aatatataat aatgacttca
    32821 atttcttttg gaatgtggac aaatggatat aagtatggga aaaaagctgt tgaaagtata
    32881 cttcaaggtg gaatagtaaa taaggttata gaaggggctt ctatactagg taccttagtc
    32941 atggggggac ttgtaggtag gtttattaat ttatctacac caataagtta tactagtggt
    33001 gattttaaat ttagtcttca aacggattta cttgataaga tatttccagg attaattcct
    33061 ttggtactta ctttattagt actctttgct ttaaaaaaag gtctttctcc aataaaaata
    33121 atgtttatac tgatagtagt gggagctatt acaggtattt taggactatt ttaaataaat
    33181 atatattggt gtaagttagg attctatatc tattgattat atttgtagga tgttggaaaa
    33241 tttatattaa aaagtttggc aattagggta attttataat agattaaatt ttaaacttat
    33301 gtaagctata tgtagtattt aatattttaa aaagtatctc agaaagattt ttaatcagaa
    33361 tgagg
//
LOCUS       DAENCV010000037        32820 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAENCV010000037 DAENCV010000000
VERSION     DAENCV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.037"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            20..721
                     /locus_tag="KQD82_001714"
     CDS             20..721
                     /locus_tag="KQD82_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4380188.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLFKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNENSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            945..2246
                     /locus_tag="KQD82_001715"
     CDS             945..2246
                     /locus_tag="KQD82_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380189.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            2383..3477
                     /locus_tag="KQD82_001716"
     CDS             2383..3477
                     /locus_tag="KQD82_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG4380190.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFLKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            3479..4612
                     /locus_tag="KQD82_001717"
     CDS             3479..4612
                     /locus_tag="KQD82_001717"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG4380191.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            4630..5739
                     /locus_tag="KQD82_001718"
     CDS             4630..5739
                     /locus_tag="KQD82_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG4380192.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            5789..6544
                     /locus_tag="KQD82_001719"
     CDS             5789..6544
                     /locus_tag="KQD82_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG4380193.1"
                     /translation="MERINMNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGV
                     LSKNYSTVEVAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFT
                     GVATSRALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMG
                     GSSIKFFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKV
                     IPHVYTSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            6549..7541
                     /locus_tag="KQD82_001720"
     CDS             6549..7541
                     /locus_tag="KQD82_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG4380194.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(7729..8610)
                     /locus_tag="KQD82_001721"
     CDS             complement(7729..8610)
                     /locus_tag="KQD82_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4380195.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            8884..10272
                     /locus_tag="KQD82_001722"
     CDS             8884..10272
                     /locus_tag="KQD82_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380196.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            10300..11253
                     /locus_tag="KQD82_001723"
     CDS             10300..11253
                     /locus_tag="KQD82_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG4380197.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            11313..11537
                     /locus_tag="KQD82_001724"
     CDS             11313..11537
                     /locus_tag="KQD82_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380198.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            11530..12042
                     /locus_tag="KQD82_001725"
     CDS             11530..12042
                     /locus_tag="KQD82_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG4380199.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            12074..12928
                     /locus_tag="KQD82_001726"
     CDS             12074..12928
                     /locus_tag="KQD82_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG4380200.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            13462..14256
                     /locus_tag="KQD82_001727"
     CDS             13462..14256
                     /locus_tag="KQD82_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4380201.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            14360..15184
                     /locus_tag="KQD82_001728"
     CDS             14360..15184
                     /locus_tag="KQD82_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4380202.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            15815..17902
                     /locus_tag="KQD82_001729"
     CDS             15815..17902
                     /locus_tag="KQD82_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4380203.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            17917..18204
                     /locus_tag="KQD82_001730"
     CDS             17917..18204
                     /locus_tag="KQD82_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380204.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            18231..19502
                     /locus_tag="KQD82_001731"
     CDS             18231..19502
                     /locus_tag="KQD82_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG4380205.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            19560..20159
                     /locus_tag="KQD82_001732"
     CDS             19560..20159
                     /locus_tag="KQD82_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG4380206.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            20164..21069
                     /gene="dapA"
                     /locus_tag="KQD82_001733"
     CDS             20164..21069
                     /gene="dapA"
                     /locus_tag="KQD82_001733"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG4380207.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            21175..21807
                     /locus_tag="KQD82_001734"
     CDS             21175..21807
                     /locus_tag="KQD82_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG4380208.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            22285..24792
                     /locus_tag="KQD82_001735"
     CDS             22285..24792
                     /locus_tag="KQD82_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4380209.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            25134..27296
                     /locus_tag="KQD82_001736"
     CDS             25134..27296
                     /locus_tag="KQD82_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG4380210.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYGDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIALDKIISNSSFNKIIKGEH"
     gene            27301..27918
                     /locus_tag="KQD82_001737"
     CDS             27301..27918
                     /locus_tag="KQD82_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380211.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            28107..28451
                     /locus_tag="KQD82_001738"
     CDS             28107..28451
                     /locus_tag="KQD82_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG4380212.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            29021..29887
                     /locus_tag="KQD82_001739"
     CDS             29021..29887
                     /locus_tag="KQD82_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG4380213.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            30000..30404
                     /locus_tag="KQD82_001740"
     CDS             30000..30404
                     /locus_tag="KQD82_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG4380214.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            30571..31020
                     /locus_tag="KQD82_001741"
     CDS             30571..31020
                     /locus_tag="KQD82_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4380215.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(31176..31553)
                     /locus_tag="KQD82_001742"
     CDS             complement(31176..31553)
                     /locus_tag="KQD82_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG4380216.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            31815..32732
                     /locus_tag="KQD82_001743"
     CDS             31815..32732
                     /locus_tag="KQD82_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG4380217.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNVTLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        12784 a         3563 c         5810 g        10663 t
ORIGIN      
        1 ataatttaag aggtgaatca tgttaagaaa tcaaccatta tatatacaag ttgtagatat
       61 attaaagcaa aaaatattaa ataacgaata taaaattgat gaaaatattc caacagaaag
      121 ggagttagag caagagtttg gagtcagtaa aattactatt agaaaagcta ttgagatatt
      181 agaatcagaa ggatatgtag agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag
      241 cctttttaat aagctatcaa aagcagagtc tttttcaagt atacttttat ctaaaggtca
      301 taagttattt aaggagacta tttcaattga aaaaataata aatgatacaa attcacaagt
      361 atttagtata tttggagaaa aatgtagcaa gattactaga atgtattatt tagatagtaa
      421 gccatatata ttttttactc attatattcc ttttgatgaa aatctaactg ttgaaagttt
      481 taataatgaa aattctttat atatgtactt atataggaga aatataaata taactagatt
      541 tgaagatgaa ttttttatag aagaatcaga actagaggta tcaaaggctt taaatctagc
      601 taaagacata ttattaggaa gaaaaagaaa gacctatgat gagaatgata atatagttga
      661 gctatcattt gcaagatata atacagaagt acaaaactat gtaattaaat acgaaatata
      721 ataagaatcc tgagtttatt gaaatgataa tatgctcagg atttttgttt agttgtaaaa
      781 ataaaaaata ttataacatt ataatgttta caaccaaaca ttataatgtt ataatatttt
      841 taataaacaa gtgatattat caattacata aaaattagtt tatgttatga aatcgttatt
      901 gcattataaa taaaggataa tatcaataat acagggggat aactatggaa aaattcgcaa
      961 gttttttaga gcgcaagatt atgcctgtag cagcgaagat aagtaatcag agacatatga
     1021 gagctgtaag agcaggtata atagcaacat taccactaac aatagtgggg tcatttttca
     1081 caatattatt aaacatacct attgatgctt atatggattt tatagctcca tttaaagcaa
     1141 tattagatat accatttaga tatactgtag gatttttatc tctatatgct acttttggga
     1201 tagcatcatc acttgctaaa agctacgatt tagatacaac aggtgttggt atgcttgcag
     1261 ttatggcatt tttagtttct acaatagttc caactcaagt agttgaacca gtaggtggag
     1321 ttatagaagc tggtagatgg atggatatgg caaaacttaa ttcgccatca ttatttggag
     1381 ctatcgtaac atcattaatt acagttgaaa tatatcgttt tatgaaggaa aagaatatta
     1441 caattaagat gccagcagga gttccagtag aggtttcaaa ttcatttact gcattattac
     1501 cgacagcagt aattttaatg ttattctggg taataagaca tattttagga tttgatataa
     1561 gtgttgcact aagcgctatg ttatcacctt taaaaggatt cttatcagga aatagtttag
     1621 gtggtgggtt actgacagta ttcctaataa ctttattctg ggtacttgga atacatggtc
     1681 cagctataat ggggccagta ataagaccta tatgggacat gtctatagca gaaaatataa
     1741 ctgcattcac agaaggagta tcagctcatg caatgccaaa catatttaca gaacaatttt
     1801 tacagtggta tgtatggata gggggagcag gggctactct tgcattagtt gtaatgttta
     1861 tgttttctaa gtcacaatat ctaaaaagtt taggtagatt aggattttta ccagggttat
     1921 tcaacataaa tgaacctata atatttggag caccaatagt tatgaatcca atactaggga
     1981 taccatttat aattggacct ataataatga caattctatc atatgttctt actattacag
     2041 gggttatacc tatgatggta gcaagattac catttgcaat gccagcacca atagcagcag
     2101 taatgagtac taactggagt atactagcag gtatcttagt attaatcaac tttgtgatat
     2161 catttgctgt ttattatcca ttcttcaaag tttttgaaaa acaacaatta caaagagaac
     2221 aagaagaaat gagtgaaaat ttataaaaat acatagatat agaggttttg tactatataa
     2281 aatatagaaa aaacttataa gttattaagg gtaaaggtag gaggaatgac ctttgccttt
     2341 aattttatat attttaaata atgtaatttg aggaggagaa agatggaaag aagattagga
     2401 atatcagtat atccagagca tagtaacatg gaagatgata aggcatatat aaaaatggct
     2461 tcaaaatacg gatttaaaag aatatttatg tgcatgcttt caattacaag acctaaggaa
     2521 gaagttaaaa aacattttaa agaaattata acctatgcaa gagaattagg atttgaagta
     2581 atccttgata tagctccaaa catatttgat atattacaaa tatcttatga tgatttaaca
     2641 ttctttagtg agttaaatgc tagtggaata aggcttgact atggatatga tggaagtaaa
     2701 gaagctatga tttctttcaa tccatatgat ttaaaagtgg aactaaatat gagtaatgat
     2761 gtagcatact tggataatat aattacttac aagccaaatg taaataatat atatggttgt
     2821 cacaattttt atcctcaaag gggtacaggc ttgccatatg atttttttgt aagatgttca
     2881 aatagattta aaaagtatgg aataagaact gctgcttttg ttaattcaaa atatgcaaca
     2941 atcggacctt ggaatataaa tgatggattg tgtacgcttg aaatgcatag aaacttagat
     3001 attgctacag caactaagca cttattcgca acaggattga ttgatgatgt aattgtaggg
     3061 aatgcatatg caagtgaaga tgaattaaaa agaatgtctg aagtaaataa atatcaagtt
     3121 gtatttaatg tagtaactga ggaaaaaact tctgaagtag aaaagacaat aatggcaaat
     3181 gagcaacact ttagaagagg ggatataacc aaccaagtag caaggtctac agaagtaaga
     3241 aaaaaatatg ctaaagaaaa tttccttaag catgataata cgaccatctt taaaagagga
     3301 gatgtagtga ttggtaatga tgactttgga aagtataaag gggaattaca gttgatactt
     3361 caagattgtg cagatgaaag aaaaaataag gtaggtagaa taaaggaaga cgaaataatg
     3421 cttttagact ttattgaacc ttggagtaaa tttaaactta ttttagaggg agagtaaagt
     3481 gaagatagac atattaataa aaggtggaaa aacagtagat aaaagtataa ttgatatagc
     3541 tatattagac gataaaataa tagaagtaaa aaatcagata gatgattcta agtatcaggc
     3601 aaatcaaatt ataaacctag atggagaaaa atttattagt gctggttgga tagatataca
     3661 tacgcactgc tacgaaagac tagaccttta taaggattat ccagatgagg ttggaataaa
     3721 aagcggtgtt actactgtag tagatgcagg aacaacagga gctttagata taggtaaatt
     3781 ttatgaagat actaaaaaat ataaaacaaa tgtatatgct ttaataaata ttgctaagca
     3841 gggaataaca tctcaagatg aactttcaag tatgatgaat attgatgaat atgaacttaa
     3901 aagagcagtt aaaaaataca aagattttgt tgttggaatc aaagctagaa tgagtaaaag
     3961 tgttgtaatt tcaaatgatg ttgaaccttt aaaagtagct aaaagaataa aaaatgaact
     4021 taatctacct atgatggttc actttgggag ttctcctcca acgatagagg atatatttga
     4081 ctatatggaa aaaggagata tattaaccca tatatataat ggaaagccaa atggaatact
     4141 tagaggcaat gaggttaaaa aagaaatcat agaagctaga gaaagaggta taatccttga
     4201 tgtaggtcat ggaaccgaaa gttttagtat ggatattgca atgaaatcta aggatgcagg
     4261 aatttttcca gacactataa gtacagatat atatattaaa aatagaataa atggtccagt
     4321 gtataatcta tctacaacaa tggaaaagtt tatatatatg ggctattcac ttgaagatat
     4381 aatagataaa gttactaaaa atgcagcaga tgcaataagt ttaaaaaata aaggtttaat
     4441 aaaagaagga tatgatgctg atttaactat atttgatgtg gttaatgaag aaaaagaact
     4501 tcaagattct ttgaataagt cagtaatcac aagtacgtct ataaagccaa gagcagtagt
     4561 agtcaatgga gaatatttaa atataggtaa gtctatagga gagaatgagt agaagggaga
     4621 aactattgta tgagtagtat atttgaaaaa tactctgtaa gacgtgttat aaatgcttca
     4681 ggcaaaatga ctatactagg agtatcaaca gtagataagg aagttattga agcaataggt
     4741 gaagggtgta aaaatttctt tataatagaa gaacttataa ataaaacagg tgaatatata
     4801 gcaaatttat tagggtgtga aagtgcacta atagtttcta gtgcatctgc tgggattgca
     4861 cagagtgtgg gagcaatgat agcaaaagat gatatgagtc tagtttacaa tgtaaataat
     4921 cctaagaaac aagttaaaag ggaaataata attccaaagg gacataatgt tgattatgga
     4981 gttccagtag aagtaatgat aagacttgga ggaggagaag ttgttgaggc cggttatgct
     5041 aatatgtgta acaaggaaca tataataaga gagataaatg aaaatacagc agcaattatg
     5101 tatattaaat ctcatcattg tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa
     5161 gttgctaaag aatataattt acctttgata gtagatgcag cagcagaaga agatttaaat
     5221 gtatattatg aattgggagc agacctagtg atatacagtg gtgctaaggc tatagagggt
     5281 cctagctcag ggttagtaat aggtaagaaa aaatacatag ataatgtaaa gcttcaatca
     5341 aagggtatag gaagagctat gaaaatagga aaagaaaata ttgtaggtct aacatgtgca
     5401 attgagagat atataagtaa tgataaagta actttaaaag aaatggagga caagctaaat
     5461 ccatttatag acaagataaa tactataaag ggagtgagtg ctagtataac tagggattca
     5521 gcaggtagag aaatacttcg tggagaaatt gactttaatg aagatataat aaataagtca
     5581 acacaacaaa ttataaatga acttagaagt ggagagatag ctatatacac tagagactat
     5641 aaggctaatg aaggcaagat tgagattgat ataagaagtg taactggaag tgatttagat
     5701 actatttata ataggataaa aaatatagta gaaagttaga gtgtagagta agtgacatta
     5761 taatgattta ataaattaaa ttttaccaat ggagaggata aatatgaatt gtataccaca
     5821 atattataaa aatagagttt gtttaaatgt tcttgcagga agcataaaaa atgctaaaga
     5881 agtatatgat gcttgtgacg ggcatgtatt gataggagta ctatcaaaga attacagcac
     5941 tgtagaagta gctattgaag atatgaaaaa atactcaaag gaaatagata atgcaatatc
     6001 tgtggggctt ggagctggag acccaaatca gtcaaatatg gtatctcaga tttctaaaga
     6061 acttcagcca caacatataa atcaagtgtt tacaggtgtg gctacgtcta gagcattgct
     6121 tggtcaaaat gatagtataa taaatggatt agtttctcca actggtaaac caggatttgt
     6181 aaaaatatcc acaggtccat taagttcaaa agaaaaagat ggtatagttc ctatagaaac
     6241 agcaatagct atgatgaaag atatgggagg aagctcaatt aagttttttc caatgggagg
     6301 gcttaaaaca aaggatgaat atatagaagt tgcaaaagct tgtgcaaagc ataattttta
     6361 tttagaacca acaggaggta tagacttaga taacttcaaa gagatagttc aaattgcact
     6421 tgatgctggt gtagaaaaag ttatacctca tgtttataca tcaataattg ataaagaaac
     6481 aggtgaaacg aaagtggaag atataggagt actttataaa ataatgaaag aattattggg
     6541 atagatttat gaatatatta gcttttggtg aaataatgat gagactttca gtacctgact
     6601 acaagttctt aacacaaacc aatgaactaa attatataat tacaggcacg ggattaaata
     6661 tattatctgg acttaaaaac tttggataca atacctatat gttgacaaag ttgcctaata
     6721 ataatgtagg taaagcatca agtgcaaata taagaaagct tggagtaaaa gatgacttta
     6781 tcacgtatgg agggaatcat ataggagtgt attttcttga aaatgggtat ggagaaagac
     6841 cttctgaagt gacttatttg aataggttaa atagttcatt ttgtgagagt aaattacaag
     6901 attatgatat tgataaatgt ttagaagaaa tggatatagt ccatatttgt ggtattgcta
     6961 tgcaacttac agattcagtt aaagagattg ctttagaact agctagaaaa tcttatgaaa
     7021 aggggataaa agtattcttt gattttaatt ttagacaatc attaaatcca gaaaggactt
     7081 atgatgattt agtaaatgat tataagaaaa tactaccata ttgtcatggg ctatttgcta
     7141 gttatagaga tataaataag atacttaata tagatagtct aaatataaaa tctagttcag
     7201 aattgattaa aaaagagtat gatatagata ttgtagctgg aactatgaga agatttgatg
     7261 atatgaataa tagatttata aagggatata tatcaaatga tgatggttat tttgaaagta
     7321 gggagtataa gttagaaata tatgacagag ttggtgctgg ggatggatat gtggcaggaa
     7381 ttatatattc atatttggaa aatatgaaga gtgaagatat agttgagttt ggggttgcta
     7441 gttcggtgtt agctcataca acttatgggg ataattcttt agtaagcaca ggtcatgtac
     7501 ttagactgat gaacaatgaa aaaatagata tattgagata atacatttgt atataataaa
     7561 atttttatct tttttagaca catttttgac ttgcttatat ttgttaaaca gagttatttt
     7621 taacgattat aaatattaaa ttagaagtta aataataaaa tagtagaaaa tagcattaac
     7681 tatagaaaaa atggttaatg ctatttttat cgttaatatt gattaatttt atattataga
     7741 aatgaacttt tctaatgctg gatttgtatt ttttttattc catacaacta caacttcaat
     7801 tctattttct acttctttta taggtactat tgcaatatca cgttctatca ttgtatctgt
     7861 aacacaaaat gaaggtaaaa tggatattcc catgttagaa gataccaatg attcaatagt
     7921 attaagatta tttgaacgtt gtaccacatt aggtacaaac cctgcttcac gatatctatt
     7981 ctgtatcata gatttttgtc catactcatc ccctgtttcc tttacaaaaa taaatttatc
     8041 atgttgtaat tcaattaaat cgatagaatc ctcattgtaa agtggatgac caggaggtaa
     8101 aagagctacc aaagaatatc tttctacagt tttatattca aaatctttta atggttgatt
     8161 ttctggatta ataacaaaag cgaggtccat agaattattt tttactaagt ttaaaagttc
     8221 ggttatttca ttagtacaaa tatccataga tatattaggg tatgtattag aaaattcttt
     8281 taaatattgt tgaagtttgt ttttttcata tcctattaag gttccaatgc tcaaagttcc
     8341 aaaaaaacca tgtgcaaatt gttgtgtttt tgcaatagca tcattgatat tttttattat
     8401 agtacgtgcc tcagataaaa aaacttttcc agctggtgtt agttcaacat gacgtttact
     8461 tcttgtaaat agagttacat ttatttgttc ttctaaagct tttatttgct gtgtaacagc
     8521 agtttgagaa atataaaatt tttctgctgt tttggtaaaa cttagatttt ctgctacaga
     8581 tataaaatat tctaattgtt tagtattcaa ataatcaccc ctatttaaac ttgattgttt
     8641 gtaaacctta tttatctata attaaagctt attatagtat aagaaaattt acttttaaag
     8701 aatattagaa aatatttgta taaaaatata tgttttaagt atatctttac attagatttt
     8761 ataaatttat taataagaat aacttatata ttccaatgaa aacgatattg gatttaagta
     8821 agtttgtctc ataaaatgaa agtatatcaa ttgataatac tttaaaataa gaagggagaa
     8881 aaaatggaag ataaatttta tgcaaaaggc aacggaaata acggatatat taaaaatctt
     8941 gaagtttgtt cctttaataa cttagatgga acttgtggaa tgtttcaaat ggctctgtac
     9001 aaaagagatg aaaaatacta tttatatgga tgctgttttg gaggaaataa aaaaaatgga
     9061 gtaatgatta gcgatattac agacccttat aatccacaat ttataaaaca ttttcaaatg
     9121 ttagacccta aagagtatcc tacaacaaca actcccaaaa ttcaaatagc agatgattta
     9181 atgatagtag caatgagttg tggaagtgga ccaggagcac ttgttgacca agctaaatta
     9241 gcaaatatta agtgtgaagc aggaattaga atatacagtt taaaagaaga ccctttaaat
     9301 cctaagtttt taggatattg ggattgtggc ttaaagcatg taatgggtgt tcatagattc
     9361 atgtacaatg gtggaagata tgtacattta tcaagtgatt gtgttggctt tgaaggtctg
     9421 atttataggg tcatagatat aataaatcct actaatccag tggaaatagg taaatggtgg
     9481 agaccagacc aatatgcaga tggatatcca aatagaactt ttgatgcagg agcacctcat
     9541 tgcccagaat ttatggataa aggatggctt catggacctc catttgtaag agacggaaaa
     9601 gcatattgtg gttatggagg agctggttta gttgtattag atgttgaaga tttaacaaga
     9661 ccaagatgct taggtgaatt gccatttacg cctgcatttt ctagtagact tgcaggtgca
     9721 agaactcata cagcattacc attgccagga agagatttag tcgttgttca aaatgaggga
     9781 gaaagattcc agttctttaa accagataac attacagatg ttcaagctat gaataatata
     9841 catatggttg atgttagtga cccaacaaaa ccaacattaa ttgctcaatt tccatatcct
     9901 gaagttccaa aagatttccc ttatcctaac tttaatgttg caggattagg aaaaccaggg
     9961 ccatttggtc cacataatct tcatgaacca atggataata agccatggtt agagcaaaga
    10021 ggagatagag tatattgctg ttatttccat gcagggctaa gggtttatga tgtatcagac
    10081 ccatattata tcaaagagct agcatatttt ataccaccaa atccaaataa aacaccagaa
    10141 gaatcttatt tcccaggatt cccaggacca cgcttggcag taacagaaga tcttatcgtt
    10201 gatgatagag gctacatcat catagatgct ttagatgatg gattctatat attaaaaatg
    10261 aaagaagatt aaataaatac taaagaggga gagttgaaaa tgaaaatact aggtatttct
    10321 tttggaacaa aaaatggaaa taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa
    10381 gaagcagggg ctgaagtaga atttattcaa atgtcatcta ttaacattaa acattgtact
    10441 ggatgttgtg cgtgtgttaa aactttatta tctgggaaag gtagtatgtg tgtattaaaa
    10501 gatgattttg aatggttgct tgacaaaatg aaagatgcag atggtatagt agtttcagac
    10561 cctatttttg aagaaggggc atcaggatta ttccatacta taatggaccg ttttggaccc
    10621 agagcagata gaggaaataa cattatagga acaaaagttg cagaagttat aggaggaaaa
    10681 attccagatt caagaatgct taatgataaa gtaatatcat ttatgggaat aggaggttct
    10741 gactggggta caagagttca atgtgaacat gctatgctag ctctcatacc aatgtggaaa
    10801 gttatagata atgcttggtt cccatgggca aaggaattag taatggatga tacaaggctt
    10861 gttcaagttc atcaaattgg tttaaatata gttgaagcag caaaagattt tgaaaaagct
    10921 tcatatcaag gagaagaagg tgtatgtcca cattgccaca atagattatt ctaccttgag
    10981 ccaggtacta aaaaggcaat ttgtgctcta tgtggtatag ttggagagtt agatactata
    11041 agtggaaaaa ctatatttag ttttccagaa gagcaattag gtcatgctca tgatacttta
    11101 ccaggtaagt ttatacatgg tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt
    11161 cgtaaaacac aagaatttaa agaaagaaaa acatcttata attttataga gccattaact
    11221 aaagaaaagc aagctggagt ttcattagga taataattaa aatactaaga ctctttttta
    11281 agggatgtta gatgttatag gaggcttatt gtatgaatga aaagccaagt tttaaacaaa
    11341 ttgaagttat tttagcagtt atttttttgg tacttacata ttacaaggtt agattaactc
    11401 cagcatttat tgttatattt ataccactat atttaagtta ttttgcagta tctatgattg
    11461 taaatcaaat agcaattaag ctattggaga taaggcaagc tagggaagct actaattaca
    11521 atataggaaa tggatagaaa gaaaaaagaa atattaacac tgtctatggg gattgcttta
    11581 ctacctcctt tatgggcagt attagcacct tatataggaa taaaaactgg agctgtagca
    11641 ctaatctgtg caggattata tgtaactaat ggtaataaac aaaaagatgg attaaaaata
    11701 atgtttggtt tttggtgtgg agatttatgg gcagtgttag ccattttaat tatgggatat
    11761 atgaatttca atcaaaacct agaattattt ttaacactta gcatattagg tttttttgca
    11821 gttgtaattg catccttatt tgaaaaaata attttcctac catcatggct ttgtggttgg
    11881 gctataggtc taactgttat gacaggtgaa aatattatta atttacaaga tatttgtatt
    11941 caaattgctg tagctatggc agtaggagta tggtacgtgg gtgcaggtgt agatttgttt
    12001 acaatatata ttttgagtaa agataggaaa aagggaaatt gaaagcatgt gaatatactt
    12061 tgattatagg agaatggcta tgggttttga atttaaaata atgagaagtt taatatatgt
    12121 aggacttgcc aaggaagaat atagacctaa gctaatggac tggttatatc gtcaccatat
    12181 tccagatagt attagcactt ttggaccata ttgtactaaa tatgcctttt atcaagcata
    12241 tcctacacca aatgaaggtg agcgttttgg tgcacgtaag atgcaactaa cagaacatta
    12301 ttggcttgta gatgaacata tgcctgagat ggcaaataga attatgacag aatatatgcc
    12361 tatggatgtt ctacgttggc aagggtgtat accagatgta gaaaataaaa gggttcatga
    12421 aaatgtagaa agtggagatg caggacgtgc agtaggtgga gataatggat gtccaccatt
    12481 tatatttgcc tttgttccaa taaactggga agaagacttt agaggaaaag gacgtactgt
    12541 acaagatgga ccaaactatc gttggcaatt tatgattaag tatccagatg gtatctctaa
    12601 agaagaagga gaaaaatggt tctatgatga ggtagtgcca tactttacaa actgttgcta
    12661 tgttaatcgt tttgtcagta gtaaaataat gattaattat ggagcaactg cttttgaccg
    12721 tgtatcagaa ctatggtttg aaggggaaga agaatggtat aaagctgtgg ttgaagagac
    12781 aaagtcgttt attaaaaaac cagaatgggc acaagaagag gagttcccat atttaaaacc
    12841 acaattcaat atcgcatcag tattcttagg tgatatagca actatggatg catactcaca
    12901 gtatcgtgga tatataccaa tgagataaaa attatagttt gtgataagta gaggaggcat
    12961 ttactcttac tggtagaaga agacttattg tatccttaaa agtaggtgaa tatatagttt
    13021 tatatttaaa tttatataaa actaagacta ataaatatga aacaatagaa ttagaataat
    13081 ttgataatat ttgttaaaag agcatttaga atgtgtttaa agattaagat attcctttta
    13141 tggtagcaga tagttgaaat aataaaactg ttatcatgaa aggaatattt ttatgtatag
    13201 gaaattttta aaaataaaag ttaaatatgt ttaagaacat cttaaagtga aagaatcatt
    13261 gttagaaata tgtagacaat atagtcttta taacgtatca tattaacaag tttacacttg
    13321 gattatgaaa taaaaagagt aattccaaag gttgtaaata ctatgctgac tttgatactt
    13381 caaagtttag gtgattttgt aattgttaat agaaatacgt ttaatgagca taaagaattt
    13441 atctaataat aggagaatat aatgtataca attggacaag tatctaaatt tttaggaata
    13501 tctagagata cactaaaatt ttatgaggac aaaggattag taaatcctaa gaaaaatgat
    13561 gaaaatggat atagaatata taatcaggtt gatatctatg atattgcaac aataaacttt
    13621 tatagagaaa ttgatataga aattaaaaag attcaagaaa taagaaaaag taagagtata
    13681 aacaatttag aactcttact agaagagaag gaacaaatca tactaaaaga gatagaatat
    13741 aaaaagcttc tcttaaaaaa ggttcagtta gtcaaagaag attgtgacaa aataaaaaca
    13801 tattttggaa aatacacaat aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa
    13861 catgttacta actatgagga atatgaagcc ctaaaagaga atacaaacaa cttaaaaaaa
    13921 gcagttacat taactgattt aagaagagta ataaatttta atgaagaagg tataatagaa
    13981 gaaaaattta taattgtaag ggaagtagat gactacaaca aaatcataga aggtgaagtt
    14041 ctatctcatc ccaattgtat ttatactatt attgaagatg gcagatggtc aactggagga
    14101 gaaaatattg ataataaagt ggaagacaga ttaagagaaa tagcaataga aaatgaatgt
    14161 gaattacttg gaatagtcta tgttaatata ttgcttacta cttatgaaga agggattgag
    14221 cgtatatttt tagagattta tgcacctata aaataatata aaagcattga cctgggactt
    14281 actcataggt ttaaactcat gatataagca gttataactt atatagcaat taaatattaa
    14341 tttgaaatga ggattactta tgagaacaga gaaatcatta aaaaggccta tagccagttt
    14401 tattattcta acaaatataa tttttttacc acttttttta ttggtagtag tgactataat
    14461 gttaaaactt ccaacagtga tatctgacat tgcgttgtgt atatcagcgt ggtcatccac
    14521 ttttgcattt atgatattat ttaaaaaaat ttatccagat aagaaatttt tagtacacgt
    14581 aaaagataga tttagaaata aacttaaatt ttcaacagta tctataataa ttagtataca
    14641 agtcctaata gctgtagtag ttatatttat attagcatct aaaaatcatg gaataatgcc
    14701 taactttaca gtttcatcat ggggagcatt tatatattta tttttaaaaa atttatttgc
    14761 aggatcatta ggtgaggaat taggttggag aggatttgtt caaaatcatc ttcagaaaag
    14821 acattctcct ttaaaagcat ccataatagt aggtttttgg tgggggatgt ggcatttacc
    14881 aatatggttt acaacaggat tcaaaggact tgatttaata aaatatatta tattttttat
    14941 gattgcaatt atatcaatca gtattataat gacaactttt tataatctaa ataaaacttt
    15001 gataatacct attgtaatac atcaactatt taatttttta atagggataa ttaatggaga
    15061 tttaatagag cttataaatt actatgcaat gttatattta atagttgcaa ttgtattggt
    15121 agctataaat ccaggaaatg ccttatataa aagattttct aaaaacacaa cacaaatact
    15181 ttaataatat aagtgagaat catatcacaa atgagagaaa taattctcct tattaattaa
    15241 ttttaattaa taaatattac aatagattta tctggaatgg aaggtatgca gagtgtattt
    15301 cattagatga aacacttaaa agaatgaaat agataagtat caacttatta tatgagagaa
    15361 actttaactt tcttggttta aaatattaaa attataagga acatttatta ttaaatactt
    15421 tatttatatg aattaattga agaaaaagga tattaaacta caagttaaaa aataaattta
    15481 atatattaga attataaata aggcacatta attttatttt aaattaatgt gccttttatt
    15541 tatggtaatt tatgcataat tagtatcgtt ttgattcttt aaataaattt ttcttggttt
    15601 cttcacataa ggaacttttt ttaatttata aaatctacag ttatattata gtatctattt
    15661 tagagaagtt taatttttgt atcaatctaa aacaaaagtt atacttaaat gaaattgaaa
    15721 acgataaaat tctcaaataa aataagtatt gtggaggtga tgaaagaatc caatgataat
    15781 atgaagacta atcttaataa gaaagtaggg gttaatggta gtggacaaag aaattgtatc
    15841 aattctccag attttattaa atagcacatt gattacaact aatggattac aagaggagtc
    15901 taattcatca aagagacaaa ttacttatag aataaataaa atcaatgaca tgcttaaagt
    15961 aaaaaatgta ccacttatat gtttaagagc tgataaagat attatcgtta gaaaagaaac
    16021 aaaaaaagca attaaagaaa ttttagaaaa aaactattct aagaatactt attattttag
    16081 taagacagaa agacttttat atatgtattt aatgcttttt attaacatgg aggatatttc
    16141 aattaatcat tttataaatt ctataaaagt aagtaggtct actgtaaatc ttgatttcaa
    16201 ggatttgata ccaaagcttg aaaagaagaa cattaaagtt aagaacaatc gtataaatgg
    16261 atattacata gttggcaatg agatggaaat taggagagtg cttattaaaa atattataga
    16321 aactttgtct aatgagaaaa gttcaagggt atttgatatt tttattaaag aatataattt
    16381 agatagtttt gaagaggcta aaaaaattat attaaagcta ataaaaaaat atgaaattac
    16441 ttttgttgaa gatagattaa tagaatttat atatatattt atctttctaa aggcaaggat
    16501 gtattcaact agaatagtta ctcaggaaaa tgtagatatt cctaatattt cagttatgag
    16561 ttcaatgaag gagtataaat ttgctaagga attattagaa gtatatggga gtgaagataa
    16621 aataaagcct cacaatataa tgtatattag tgcttggatt ttaggtatat cagtaggaaa
    16681 ttatgaagaa gatacagaag ataaggatgt aatttcaaaa attgtaaata aaatgatgac
    16741 aaagtttgga tatttaagtg gagtttacta tataagccaa gaaaaaatat ttaaacaatt
    16801 atattctcat tttagacctg cctactatag acttttattt aaattaccta tctataatcc
    16861 gatttgtgaa aaagtaaaag aagaatataa attagtttat agaattgtaa gtaatgcaat
    16921 gaaagagttt tgcggattat ttggagaaga aattcaggaa gaagagttag catatcttac
    16981 aatgcacttt gcaacacttt tttcaaataa aaaagaattt gatggtttta gaaaaagaat
    17041 agcattaata gtttgttcaa atggcgttgg tagttcagct attttacatg cagaacttac
    17101 aaacttattt cctgacttac actttatacc aatgtcggaa tcagttggat tagaaaatat
    17161 aagtgaacct gtagatattg tatttactac taattataac gcagagggtt taaaaacaga
    17221 tgtaccagta attaaagtaa gccctgttat gaccacaaaa gaaaaatata agattacacg
    17281 tgaagtgtat attcaattag gtgatatttt tttaagacaa cctaaaatag atgaggttat
    17341 ggatataata aaaaaatatg caaatgtaac ctctgaaagt attctttcaa gtgagctatt
    17401 agcttatttt acacagatag agaattttac atctaaggaa ggagaggggc ctatgctaag
    17461 tgagattact aatgaaacac tagtaaaaat aaaggtaaaa gctaagaatt gggaggaagc
    17521 tattagacag tcagcttctg tattagttga aaataacaaa gtaactgaag ggtatgtaga
    17581 tgctatgatt aactcagcaa aagcatctgg accatacata gtaattacaa aacatgttgc
    17641 acttccacat gctagaccag aagctgggtc aaaagagatt gcaataggca ttgctacttt
    17701 agatagtcct attgagtttg ggaataacga aaacgaccct gtaaaatatg tattttgtct
    17761 aagtgcaata gacaataata gccatttacg tgctatgtct gagctagttg aactattgga
    17821 agaagatgag ttttttaagg ttttagatag tgctaaagaa gctaaagaaa ttattgattt
    17881 tataaaaaca cacgaattat aaaggaggat ttaattatgt ataaaatttt aatcgcatgt
    17941 agagcaggag ttggctcaag tttaatgtta aaaataaaga ctcaacaaat tattaaggaa
    18001 aataactttc caattgaagt agagcatggt tcattggatt cactaaatgg attcaatgga
    18061 gatgctgtag aaacattaat tgatgttgct gaagaattaa aaaataaaaa tttgaagttt
    18121 gatataattg ggattcacaa tattgttgac cgtaatgaaa taaagacagc actagaaaaa
    18181 ttccttgctt caaaagaggg ataaatttaa gatgataagg aggaatattt atggagttta
    18241 ttattagttt gctgagtaca cctgctgttt tgcttggcgt agttgcatgt ataggattga
    18301 ttcttcaaaa gaaatcagct gttgaagtat ttacaggctc ttcaaaaaca cttatcggtt
    18361 ttttgatatt tggtattggt gcctctgcta tgacaggagc acttcaaaat ttcaataaac
    18421 tgttccaaca tggctttgga attactggtg ttatagcttc accagaagca gctacagcac
    18481 ttgcacaagg tacatatggt tttgcagttt catgtacatt aattcttgga ttcatattaa
    18541 atttagtatt tgcacgtatt acaaagttta agaatatttt ctttacaaca ggtcatagtt
    18601 tgttcttttc ttgtgtatta gttttaatta tgaaagcaca tggatttgat aatacaatca
    18661 ctattttaat aggtggaact atcttagggt ttatgtctgc tgcactacct caattctgtc
    18721 aaccatttat gcgtgaatta acaggtggcg acgaacaagc tattggtcac tttaacatga
    18781 ttggttatgg tttatcaggt tatattgggc gtttattttc aaaacataaa gatgatacaa
    18841 cagagactat agaatttcct aagtggttat caatattcag agacttctta atgggtctat
    18901 ccatagttat gttaatctta ttttatattg caacattaaa agctggtcaa gcatttactc
    18961 aagaaattgc tggttcaaca cattggttgg tttttccgct tatagaggca tttacatttg
    19021 tggcaggtat gtcaatattg atgtcaggtg ttcgtatgtt cttagctgaa ataactgcag
    19081 catttgtaat catttcagaa aagtatattc caggttcacg tcctgcgcta gatgttccaa
    19141 ccgtatttcc atatgcacca aatgcagtta ttattgggtt tatatcagca tatgctgctg
    19201 gtctattagc aattgctatt atggcatcat ttcctagtat atttcctgta gtaattattc
    19261 cagctgctca catatgtttc ttctcaggag gtacagctgc aatatttggc aatacttcag
    19321 gaggatggag aggggctatt gctgggtcat ttgttgtggg gttattactt gcatttttgc
    19381 cagttatact atatccagtt tatgggactc ttggtataga aggagcaact ttccctaata
    19441 ttgattacaa cgtagttggt tcgatattac ataatatttt acaatttata aaacctattt
    19501 aaactaaatt atataaaagt atagtcatag ttactatgat ttcaaggagg atacatctta
    19561 tggaaaatac attatctgaa tttttagata atatatctaa tgaattgtca gaatttattg
    19621 cctcaataga tgagacatct ataaataaag catgcgaatt aattcttgaa gctgaaaaaa
    19681 atcacggacg tgtacatgtg actggtattg gcaaaccagg acatgtatcg ggatatattt
    19741 catcattgct ttcatctact ggaacgtcag catatatatt gcatggtaca gaagcagtac
    19801 atgggtcaag tggtcaagta gttgaaggag atgttgttat tgcaatatca aatagtggtg
    19861 aaactcaaga gttaaaagca actcttaaga ctctaaaagt aaatggagca aagataattg
    19921 gtgtaagtgg taatgagagt tcatttttaa aaaatatttc tgatatattc ctgtttgcag
    19981 gtgttaagca ggaaggagat tgtctcaata aagctcctcg tgcttcaatt ttagctgaaa
    20041 caataatatt acaatcatta agtgttgtat tacaatatgc taaaggtctt aatacacagc
    20101 agtatttgaa gtggcatcca gcaggaagtc ttggaaagtc tattagagag gggatataat
    20161 tttatgaaat ataaagggat tatatgtgca atgataacac catttgatga aaatcaaaat
    20221 attaattcac aagcaacatg tcaattaatt gattatttga ttgaaaaggg aatttatgga
    20281 ttatttattt tgggcactaa tggtgaatgt catgtattaa cagatgatga aaaagttgaa
    20341 tttgcaaaga ttgttattaa tcatacaaat aatcgtgtac ctgtttttgt tggaacaggg
    20401 ggtaattcaa ctcgtgaagt tgtagattta tctaagagaa tggaagcgat tggagcaagt
    20461 gcattatcta ttattacacc atactttgtt acaccaacac aacaggaatt aatcttacat
    20521 tacaaaacta ttgctaattc agttaattta cctattataa tgtataatat gccaggtaag
    20581 actggaatta atattgaacc agagtcagta cgtgaattat caaagataaa gaatattgtt
    20641 ggaattaagg atagtagtgg taaacttgac aacatgaaag cttatattga agcaacgaag
    20701 aatgagaatt ttagtgtgtt ttcaggctca gattcactta ttctggatag tctaaaagct
    20761 ggtagtcaag gagctgttgc agcaacaaca aacttcctca cagagattgg tatctctatc
    20821 tatgaaaact ttatgaaagg ggatttggaa gaagctcaaa aagctcaaag tagtatagaa
    20881 gaacttcgac gtatactgaa acttggaaca attccatctg ttatgaagaa aactattgtc
    20941 ttgaatggaa ttaatgttgg aactgcacga cttcctgtaa ctgaaccaac tggggaagta
    21001 cttgaagaaa ttaaacgagt agttgaaaat tataggacaa tacttaataa aagtagtgag
    21061 aatagataat ataaataggg aaattacact ggtatataat taatatatat attataaaac
    21121 atgactaaaa ttaatgaata agtaaagtgg tttgatgagt atagaggtga taatatgaaa
    21181 aagatgaata taaccaaaag aatgtcagaa tgtggggctc ttgcaattgt aagagaagaa
    21241 aatcttaacc gtgcatgtga aattgctgaa ggatgtataa aaggtggtat aacagttata
    21301 gaaatgagtt ataccctaaa taatgctgga gaaataattc aagggcttaa taaaaagtat
    21361 ggtgaaacac tttgtgtggg agcaggaaca gttttggata gtgaaacagc tcgtcatgct
    21421 atattacatg gagctcaatt tattattgca ccaaattata atgaaggtgt agcgaagatt
    21481 tgcaatagat atcagattcc ttatgctcca ggatgtacat cattgactga ggcagtagac
    21541 gcactaagtt taggagcaac ttttataaaa gctttcccaa tatcagattt ctatggacct
    21601 aaactagtaa aggtatttaa gacaccaata cctgatatgc cagttttagc aagtggaggg
    21661 attaatattg aaaacctaca tatatggtta gaaaatggag ttgatgtatg tggatttggt
    21721 ggtttattaa ctaaaggttc tgttgaagat attgctaaaa atgctaaaaa gattcgtgaa
    21781 atcattaaaa atataagaaa aatttaacat agggctgtat catagtatct tataattact
    21841 atgatacagc ctttttaata ttattcaaat ttaaaacagt ttacatttct attatattac
    21901 atataatttc agtaatttta tcttaatttt tctatctgga aaaaaatgat ttaaaactta
    21961 agttatgata taatttcatt aaaagaaggc gatttttagt aaatatgaga aaataatgtg
    22021 tttttagggg aaaagttaaa tacagattta agtgaacaag aatcgataat taagatatta
    22081 aatggtgaat agggcatcaa aaaaattact aaattaaaag ttttagaggg agaatcttag
    22141 caagtgtgtg tgaaaattca atttcaaaca aaaaataatt gtaaatgaaa tcaatttata
    22201 cacgttgaag attcactttt atcattatat taaaaagcag agtgaattaa aagtgagtct
    22261 aaagatagga ggataattaa aacaatgaaa gaagacatta ggttacatcg aatgtgtaaa
    22321 aaagaacaag caacaaagaa tgaagttata tcagaggata ttctaaaatc tatgagtatt
    22381 ggggtcagta aacatttttt agatgattca tttacaataa tatggggaaa tacatcattc
    22441 tatcaattat taggatatac agaagaagaa tttttagtta aattttctag tttaaaagat
    22501 tattatgaac agaatcttta tcattttgaa tctatgaaaa atcatttttt agaggcttat
    22561 catcatggcg aaaagagtat agaatatcaa atttgcattt cagcaaataa tggagaccct
    22621 gtttgggtaa taatgactgg tatattaatt gaatcacaag ataatgagaa atcaatggtc
    22681 tatatattgt attcaaaaat taatgagtta aaaataaagc aagaagaact atctgatatg
    22741 gaaaaagaga gtacagagaa ttttaaatgg atgatgtcag agtatgctgg gaatgtttat
    22801 attagcgata tggatacata tgaattacta tacttaaata agcactcatg caatacttta
    22861 caggcatcag caaatgaact tattggaaga aaatgttatg aggtaattca aggtagaact
    22921 tcgccatgtc cgttttgtac aaattcgtat ttaaaaaaag atgacatata tgaatgggaa
    22981 ttttataata aaaaactgaa acgaacattt ataattaaag accgtatgtt gaattggcaa
    23041 gggcgaagag ttcgtattga attatcttat gatatgtata gtgctgaata taaattagca
    23101 aaaaaagatc aggaaagaga atcaattttg aaaactattc cagcaggaat ggttagatta
    23161 gatgcaagag attatcttac tatactttgg tgtaatgata tatttttgaa tatgattgaa
    23221 tatacaaagg aacagtttgc agaggaactt aataatcaat gtggatattt attttctgaa
    23281 gattataaac gtagtaagaa actggcacaa gatttgaagg agtctgggga taatgttgta
    23341 tttgaagcaa aaatatatac acgttcaagg gaagaacgta tatggactgt tacattgtgt
    23401 tatatcagtg gggaagacag ttgggatgga ataccatctt tttatagtat aggtattgac
    23461 ataacaaagg aaagaaaaca gattgagaaa ttacagcata tatcagagaa agatgcatta
    23521 acaggaattt ataatcgtgc tgagactgaa aggcagatta agaaatattt tgaaaaaaat
    23581 ttaaatgtta tgggtgcact gtttatgatt gatacagata attttaaaca gattaatgat
    23641 acagaagggc atatgattgg agatatagta cttactgaga tggcatcagg tatgaaaaaa
    23701 ataatgcgtg acagtgatgt tgttggaaga ataggtggag atgagtttac aatttttatg
    23761 aagaatattt cctcagttaa agatgcagaa aagaaagcag aagaactatt gtatatgttc
    23821 cgtcatctat ttcagaaaga aaaaagtttt ctaaaagtaa cttgtagcat aggaatagct
    23881 atatatccaa aggatggtac tacattcaag gaaatatatg caagagctga taaagccctg
    23941 tatcaagcaa aaaatatggg aaaaaataat tatgtaatct acaaccaaga ttattttaaa
    24001 gaattagaag aatttgattg ttcttctctt ggtacagtta ttgattcaga aaaacaatac
    24061 gctgaatatc ctgataatct tacacgttat atattccgta tgctttatca gactgatgat
    24121 atagaccagg cgattaatat gatacttgag gttgttggga aacagtttga tgtaagtcgt
    24181 gtttatattt ttgaaaatac agaagatggt agatatactt ctaatactta tgaatggtgt
    24241 aatgaaggaa tctcttctca aatggcttat ttgcaaaaca gcaattatca agactatgag
    24301 gattatgaaa agctttttga ggatgaatta gttttttatt gtcgagatat ccatacatta
    24361 agtcctaaac tagaagaatt gttttctagt caaggtatcc actctacact tcaatgtgca
    24421 tataaagaag atagagtttt cagtggattt gttggatttg atgaatgcac aggactacgt
    24481 ctatggtcac aagaggaaat aagtacatta tcattgattt ctcaaattat atcaatgttt
    24541 ttacaacgta aaaaaaataa taaacttaat caacagattc aacaatacca gaatatttta
    24601 gataatattg accagtgtat ttgtgtgatt aataaagaaa ataatttttt attatatgta
    24661 aataaaaaat ttaaaaatac atattctaaa ttccaaattg gacaacctta ttatcttgat
    24721 tctatagata ttcataagat atccattata tggaataaaa aagaagcata cctatgtact
    24781 agcacgattt aatggtaaag taaagtttat attggactta aattaaattt cgttttaaat
    24841 aagatattat tttagttgct tactgaaaag actagaatct atattaatga attatactaa
    24901 ttttatacca ctattaaaat attgtttgct aaaaataaaa aatagcctta aaacagttga
    24961 ttataaaaaa ttaactgctt taaggctatt tttatctttg tgacgaaata gacacttgcc
    25021 atttagaatg tgacaatata gtagttgttt agaaaaggca caaagcatta tactttaact
    25081 aaagtaaaat gtattttgct tttagaataa aaacaattag gagggagcta taattgaaaa
    25141 agataatata tgtattattt acaattgaac tattaatagt atctttatta ggtctaaata
    25201 tagttaaaga taatgaaatt aataatattt tatataacga tacaacaagt atatctgtaa
    25261 tgtttaaaga ttataaaaag ttaaataaaa attatagtaa gtggattaaa gatattgcag
    25321 atgaaaataa tgtgactata tcaaagtatg tttttaataa taatgaaaaa ttatctatat
    25381 atacgactga tacaagtttg aataacaata taaagttaaa atcaggaaaa tttcctaata
    25441 ctaattcaaa tgaatttatt agtaatataa acactaataa tagtaatcaa gtaggtaaat
    25501 tctcaactat gagtaaagac atatcaattt taattaggga ttttggtaaa ataagtgaag
    25561 ttggagaatc aggaatcttg tatatttcaa ctcaaaatga agatacaata aataaaatat
    25621 taagagaact aaatgtagac agaagtattt ttgtagcaag attacatgat agatatgtta
    25681 actcaaattt atacttaaat ccttcaataa ttagagattt aatactaatt atcctttgtt
    25741 tcttggctac aataatacat tattcaatta gtgtgagtag agaggcttct attttaagat
    25801 taaatggata ctctaagcta gatataattg ctagagttat aaaaaatata cttagaatta
    25861 tgattttatc atctgtcaca gcattattaa tatatataat ctatatttta aaattaaata
    25921 taggtgttag agcatgctta tactttgcta tgttttcgat tatatgtatt ttgataaata
    25981 ttttgatatc tatattaata gttggtttta atagtagaag ttctaaatat gtgctatctc
    26041 taaatggaaa aaaatcttat ggttttgtaa atataatgca tttcacattg aaaattgtat
    26101 ttgtatcatt tcttatatta tccataaata actgtatttt aaattataat atgctacaaa
    26161 ctcagttagg aaatctatct gagtgggata aagcaaaaaa tgtttataat cttactctta
    26221 aagatacagg cgaacaatct ttaggaaagg aagaagtaat taaaaatgcc aagattaaag
    26281 gtttctataa aaatttggta gaagaaaaag atgctttctt aatagattca actaattttg
    26341 aaaaacttga agatggaagt tacatgtatg aaataaattc aaagggtaaa aacccagaag
    26401 taagtcaata tggtaaaaat ataaaaataa ataaaaacta tcttaaagtt aaccctattt
    26461 actcaaatgg caaagaaata tttaatctaa tcgattatgg cgataagact ataaatttat
    26521 tagttcctaa aaagttacaa gtacatgaaa atgagattaa aaaagagttt agagaattat
    26581 tttattttga aaaaatagaa gtagaaaata tgtacaataa agagttaaat agagagttaa
    26641 ataaaacaaa gaagtcagat ttaaatgtta atataatata tgtagataat aatcaaagct
    26701 attttactta taactccctt gttatggatg acaataggaa tctcattgat gacccaatag
    26761 caatagttga catagataat attgatgatt ctttttatct ttcttatata tctagatgtg
    26821 tatattttaa ttctaagaaa ttagatgccc ttgcagatat aagtaacatt attgaagctc
    26881 aaggtgtaga agcgcatatc caaagtttac atgctgttta taatgagtat ggtttagaaa
    26941 taaataaact agaaaaatat ttgaatagtg aaatttttac tataataata atagcaatat
    27001 caaacttaat gattacctac aatatagtag ctagctacta tgaaagaaat aaatataagt
    27061 tatatattaa aaaaatattt ggttattcaa ctacagctag aagtatgttg ttaatagttt
    27121 cattaatatt aacaaacatt attccaatag gcattatatc aacaagagta gatttaagta
    27181 ataatattat cttatttgga cttctaatat tagttataga agtaattgta tctattgcat
    27241 tagacaagat tatctcaaat agttcattta ataaaattat aaaaggagaa cattaaaaag
    27301 atgatttcat taataaatat atctaaaaaa tttaaagata aatctataat agacaaattt
    27361 aacctggaaa tttctaaggg cgagtttgta gcgataacag gaaatagtgg ttcaggtaag
    27421 acaactttac ttaatataat gggattatta gaaaaaccta attctggtga tattataata
    27481 aataatataa aaaatccaaa tagtaaacag ataagaattt tacaaaggga tttttatggc
    27541 tatctatttc aaaattatgc tttaattgag aatgaaaatg ttgaaaataa cttgaaaatt
    27601 gctttaaagt atcaaaaaaa tatcaaccat aatgaaaaaa taaattacgc acttgagtct
    27661 gtaggtctta gaaaatacaa taaaaaaaag atatacgaat tgagtggagg agaacaacaa
    27721 agggtagcat tagcaagagt aatattaaaa gaatgtgaag taatatttgc agatgaacct
    27781 acaggtaatt tagataaaaa aaatagagac atagtatttg atatacttaa aagtctaaat
    27841 aagaatggta aaactatcgt atttgtaact catgatttag agttatcgga gcaggcagat
    27901 agagttataa aattgtaata atatcaacta taataaatat aagaaagaga ttacagctaa
    27961 atcaagttta ataataatac gaatagaatt taaacattaa gtaaattagt agtttgtaaa
    28021 tataagtata atatgatatc ataaaggctc aaaagaaatt ttatataata agaaatctaa
    28081 aaataacaaa tggaggacat ataaagatgg aaaatttacc aaattgcccg aaatgtaatt
    28141 cagaatatac ttatgaagat ggaactcttt tagtttgtcc agaatgctct catgagtggg
    28201 cattaggatt gggagctgaa gaagattcaa atattatcaa agatgtaaat ggaaatgtat
    28261 taaatgatgg agattctgtt acagtaatta gagaccttaa agtaaaagga agttcatcat
    28321 ctataaagat tggaacaaaa gtgaaaaata tccgtttaat ccatgactca tcagatggac
    28381 atgatataga atgtaagata gatggttttg gagctatgaa gctaaagtct tctgttgtta
    28441 aaaaagcgta atattataaa taagctgtag tgtttattgt ttttcagtac agtataaaac
    28501 cataagaatc tattaagtaa taaaagtttg tttatttatc tttatatttt cttatggttt
    28561 tattaaatat aaattttatt gaacaccaaa atattctctc ctctacaaat aagccaatct
    28621 tatttaaact aatatacttt gtactattta tactgaaatt gtaaatttaa atgctatgtt
    28681 atttaggttt gtatagttaa tttatatatt tctgatttta taaattgtat ttattattat
    28741 atcttgattc taaaatcatt aaaaatttca acttctataa agatttacaa tttatcaaat
    28801 acattatgag tatataaaca atttattatt acagtgctac atttaaaata agtcttagta
    28861 taaaggtatt gttacaagta taaagaaact tatgtatgct attggaataa ataatacctt
    28921 aaattataat taagaataag aactgttaaa taagggactt gattatcatt tagttataga
    28981 taatataata ttaaaaataa agtttaagag gagaaaaaat atgatagata tacataaaaa
    29041 tatatatgat aataagttat ttgaggagct aaagattgac tgtaagaagt gctttggact
    29101 ttgctgtgta gctctttatt tttctgcttc agatggattt cctatcgata aggaatcagg
    29161 aaaaccctgt attaatttac aaccagattt taaatgttct gtacataata gtcttatgaa
    29221 gcgaggtttt aaagggtgta ctgcatatga ttgttttggt tcaggtcaaa aagtagcaca
    29281 agtaacatat aaggggatag actggatgca atcatcagaa ctaacaaatc aaatgtctga
    29341 agtgttttta attatgagac aattacatga aatgttatgg tatcttaaag aagcatctgt
    29401 attaaatata tcagatacaa tcaaatcaaa aattgattta ataattgaag aaactgaaaa
    29461 gataactaat atgggcccag aacagataat taatttggat atcatatcac atagaacaaa
    29521 ggttaattta ttattatcac aagcgagtga gtctgtgatg ggaaaggtta aatcatttat
    29581 caagacttct actcttaaaa acatgaaaaa attaagtaaa aatatagatt taataggagc
    29641 agaccttaga aaaataaatc taataggagc agatttaaga ggtagatttc taatagcagc
    29701 aaatttaaga aatacagatt tgagtggggc aaatttaata ggagcagact taagagattg
    29761 tgatattaga ggtgctaatc ttgaaaatag tatattttta acacaattac aagtaaatac
    29821 tgcaaaagga gattctagta cgaagttacc agcttcacta atacgcccta aatactggga
    29881 aaaataatta aattaataca taattttaca aaatatgaac ttttttgact ttaattcata
    29941 ttatacatta gagattcttt acaagaattt cttaatataa tatagggaga gatataaaaa
    30001 tgagttatca aaattataaa tgtgaaagag attatgtgta tgatgattat aaatacaata
    30061 agtgcaacaa gtgctataat gattatgagg aaatgacaca tgttcatgaa tattcagaaa
    30121 gtgttaagtt agcagaagaa tgtgaggata gacataacca ccgtgcagca ggggttactg
    30181 gtgaagctat accaataaat ggaggtacaa atcatgttca taaaataaat gataatgtag
    30241 atttccttga ccatttccat aagatatgtg ttacaacagg gccagcaatt agaatacctg
    30301 gaactgataa acacattcac ttaatatgtg gagaaactac tgttaatgat ggtcattgtc
    30361 ataaattcct ttttactact caaatagaag cacctttagt ataagctgat gtgaattatt
    30421 gtagtgaatt tgctagatta tagtataata atatataata gataatattt aaacttaatg
    30481 aggactatga tatttaacct tcgtagtcct ttctaagttt tgtataaaaa agtgttatgc
    30541 aattaaaagt gatataggga ggaaattttt atgaaatctg gaataattct tgaaatgaaa
    30601 caaataaatt caacagaata tgacgaaatg ttgcaagtct gggaatcatc agtaagagca
    30661 acgcatgatt ttttaacaga aaaagatatt gaatctttga taccattagt tgaaattgga
    30721 cttaaagaag tagaaaatat agtttgtata aaagataatg atataataaa gggctttata
    30781 ggaatagata aagataaaat tgagatgctc tttatagaag ataaatatag aggaaatggt
    30841 attggtaaaa aattaataaa atatgctata gataaataca atgtaaaata tgttgatgta
    30901 aatgagcaga ataaaaaagc tgtaggattt tatattcact taggatttaa agtgtttgat
    30961 aggtctgaaa tagatggaca aggtaaccca tttccaatac tacatatgaa attaatttaa
    31021 agttatactt ctcacttatt cgcaaaaata tttacttaaa tttttataca aataaaagtc
    31081 gtgacagttt taggacacga cttttttact acactattaa attgaaattt atctgttcta
    31141 tattatgatt attagatgta gtttttttat tttaactatg tacttaaata ccaagtatac
    31201 ccatacctat caagtacttc agcagcgcca tcattttgat gtggtggtgg gtgtggtaaa
    31261 aattcttgta caagtgtacc accttcggca agttttacag atatttcttc tatcgcttct
    31321 cgagatggta gaacaacaca aatcgccatg ccagaaatta caacaggctt gtctgtatcc
    31381 gcacccatta tacaaccttc gtattttgtg aaagtaacac ttgcgtgcat aataggattg
    31441 tttggattgc tgtctaatcg cgcaaatata gtttttcctc ctccgaaagc ctcagcataa
    31501 aaattaaatg ctttttcaca agtaccatta aatattaaat atggagttag catgaccaat
    31561 atatcctcct tgatatagaa taatttatag ttgcacctga taatctaaat tatgatttag
    31621 taaattgtat catagggaaa acttgaagta tataattttt tattattata ttatacaaat
    31681 ttattaattt aaaaaaatgt tatatgtacg gaaggtttac aggataaaat ataataaaaa
    31741 gattaaggtg acattataaa aaatgtatat tgacattgac gctacgtaat tgtatatact
    31801 ttgtcttaaa ggagatggta aatatgaata ttatagaaat tcgctcagat aaaatataca
    31861 agaagataat ggatgcacca ataaacaaaa aagaagatat atacagatat gaattgatga
    31921 agccttttga atttaagtgg aagtgtatga atgttccaat agttgctaga cagaaaggtg
    31981 gatatgatgt aattatagca agtgaaatgt taggggtttt atcgcctaag gatattgatg
    32041 aaaagcaaaa aaagaatata aatgtgttat ctgctgataa aatttgggcc acttgtaaag
    32101 aaaccataga aaactctata aatgctttta taaaagaagg gtatgattta aacattaagg
    32161 actataaata ttcaatatta ttggcgaatc caaatagtcc ttatacaata ttaagtgatg
    32221 gatactgggg tgatggtggg attcctggat atatatttct atcattggtt cctaatgaat
    32281 atactatcaa tagattacca gtattaatag cacatgaatg taatcacaat attagatttc
    32341 agtttataga gtggaataat aatgtaacat tagaagaaat gatgataaat gaaggtcttg
    32401 cagaaaattt tgcaacatgg atgtttggag aggaaatgtt aggaccttgg gtcagtagaa
    32461 cagatatcga aacattaaat acttatataa agccaataat aaaaagtgct ttaaaagaaa
    32521 ctggatttca aaatataaca tcttatcttt atggtgatga tatagctaaa atgcaaggat
    32581 attttccagt agggttgcct tattgtgcag gatatgcttg tggatattat atgattaagt
    32641 attatttaga aaagacaaat aaatcaataa tcgaagcgac tttattgcct tatagtgaga
    32701 taatcgaagc agtaaaagag ttttgggaat aaattttata aattgcataa tttgaaataa
    32761 attatgcaat tattttattt ttaagaaaaa ataaattgac aataatttat aagaaatata
//
LOCUS       DAENCV010000038        31438 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAENCV010000038 DAENCV010000000
VERSION     DAENCV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.038"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            379..2613
                     /locus_tag="KQD82_001744"
     CDS             379..2613
                     /locus_tag="KQD82_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4380218.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSKKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2644..3489
                     /locus_tag="KQD82_001745"
     CDS             2644..3489
                     /locus_tag="KQD82_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4380219.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3729..5462
                     /locus_tag="KQD82_001746"
     CDS             3729..5462
                     /locus_tag="KQD82_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4380220.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5788..6672
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5854..5882
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7202..7306)
                     /locus_tag="KQD82_001747"
     CDS             complement(7202..7306)
                     /locus_tag="KQD82_001747"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG4380221.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7931..8176
                     /locus_tag="KQD82_001748"
     CDS             7931..8176
                     /locus_tag="KQD82_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380222.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIISFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8929..9075)
                     /locus_tag="KQD82_001749"
     CDS             complement(8929..9075)
                     /locus_tag="KQD82_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380223.1"
                     /translation="MVFETFDKAIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            9108..9452
                     /locus_tag="KQD82_001750"
     CDS             9108..9452
                     /locus_tag="KQD82_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380224.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSTKNQVDFIKLVLKESYRLKEISENI
                     CDFVTTPIKKIV"
     gene            10361..11716
                     /locus_tag="KQD82_001751"
     CDS             10361..11716
                     /locus_tag="KQD82_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4380225.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFVICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLAFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11834..12502)
                     /locus_tag="KQD82_001752"
     CDS             complement(11834..12502)
                     /locus_tag="KQD82_001752"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG4380226.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVENIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12569..12997)
                     /locus_tag="KQD82_001753"
     CDS             complement(12569..12997)
                     /locus_tag="KQD82_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG4380227.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            13104..13697
                     /locus_tag="KQD82_001754"
     CDS             13104..13697
                     /locus_tag="KQD82_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG4380228.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13816..14751
                     /locus_tag="KQD82_001755"
     CDS             13816..14751
                     /locus_tag="KQD82_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG4380229.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMVETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(15022..15966)
                     /locus_tag="KQD82_001756"
     CDS             complement(15022..15966)
                     /locus_tag="KQD82_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG4380230.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            16284..17714
                     /locus_tag="KQD82_001757"
     CDS             16284..17714
                     /locus_tag="KQD82_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380231.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17914..18498
                     /locus_tag="KQD82_001758"
     CDS             17914..18498
                     /locus_tag="KQD82_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG4380232.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18602..19381)
                     /locus_tag="KQD82_001759"
     CDS             complement(18602..19381)
                     /locus_tag="KQD82_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4380233.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19439..20695)
                     /locus_tag="KQD82_001760"
     CDS             complement(19439..20695)
                     /locus_tag="KQD82_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG4380234.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20953..21852
                     /locus_tag="KQD82_001761"
     CDS             20953..21852
                     /locus_tag="KQD82_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG4380235.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21876..23324
                     /gene="ddlR"
                     /locus_tag="KQD82_001762"
     CDS             21876..23324
                     /gene="ddlR"
                     /locus_tag="KQD82_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG4380236.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIATIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            23383..24189
                     /locus_tag="KQD82_001763"
     CDS             23383..24189
                     /locus_tag="KQD82_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG4380237.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            24215..26134
                     /locus_tag="KQD82_001764"
     CDS             24215..26134
                     /locus_tag="KQD82_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG4380238.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(26376..26723)
                     /locus_tag="KQD82_001765"
     CDS             complement(26376..26723)
                     /locus_tag="KQD82_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG4380239.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26710..28638)
                     /locus_tag="KQD82_001766"
     CDS             complement(26710..28638)
                     /locus_tag="KQD82_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG4380240.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(29049..31238)
                     /locus_tag="KQD82_001767"
     CDS             complement(29049..31238)
                     /locus_tag="KQD82_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG4380241.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT        11803 a         4422 c         4001 g        11212 t
ORIGIN      
        1 atgtataaaa agttaattat gaataaaata attatgcctt tgaaaaatca ttaaacttaa
       61 aattaattat aaaaaaactc taccttacta actatacagc tcacaatcta tctatttatg
      121 aaaaaatatg atataattgc attcaaaaag gaaatctttc aatatactag aatttaagca
      181 ttaactaatt ttatctaata ttattactaa actaaaaaat atttgtattt gtagcattgg
      241 agcaagtaac ttaatgtata ttcattttaa atacttattt aaaatttagc atttaaatta
      301 tttacaaatt aacatttaaa tacttctagc ataaatacag ttgtcatcaa aatttaaagt
      361 atcttatgga ggaataccat gcaaaatcat aattatataa aaatgttaaa taaaaaggta
      421 gaagtattat ctaaggaatt agatagtaga aattttaaat taaaaacctt aaatgaaata
      481 gatttttttt ctaaaatacc aaacagagaa tttatgtacc aacatattga tgactgtatt
      541 tcgaaaaata tacatatagg aatcatgtta ttaaatatcg ataactttaa attatttaat
      601 gatatatatg gatatctagt cggagaaaat ttattaatta ctctaactca aaacctattg
      661 cactttattc cagaatcaaa gggaatagtc gcttatttaa aaggagaagg ttttttaatt
      721 gtacttccaa atgtatcaaa taaagagtta aataatatgg gtgcaactat tatagagagc
      781 gcccgagaat taaaaataaa acttcctaat gaaaaagaaa tatactccaa tgttacattt
      841 agtatcggca gcactatctg gaatggtgaa ccaaacatga ctactttgat gcttttcaac
      901 caagtatata gtgctttaaa aaaggctaaa agtgaaggca aaaatagata cattctattt
      961 gattttaaag ataatagttt ttacaataaa ttagaaaatg ataaggagtt cttatcagaa
     1021 atatcacatg ttctacaaac taaacaaatt gaagtatttt accaaccact ttataatata
     1081 aaaaataata ctatagttgg atgtgaggca cttgcaagat ggagacaccc tattaaagga
     1141 cttcttactc cagataaatt tattccttta tttgaacagt ttggtctgat aacggaatta
     1201 gatttgcatt tatttgaaga gaactgtaaa aatataagaa gatggattga taacaatgat
     1261 gtttttgttc caatttgttg taacttttct cgtcttcatt tttttaaccc taattttcca
     1321 agtatgctaa aaggtattac tgaaaagtac agcgtctcta catcaaattt tagcattgaa
     1381 ataacagaaa atatattaat agaaaataca aatacaatta ttcaacaact agctgaactt
     1441 cgttcttatg gtttttctgt atcaatggac gattttggtt ctggatattc atcttttggt
     1501 atgttgcaaa atctaccttt tgatataatc aaaattgata ggatattttt atctcataat
     1561 ttaaatgatt ttaaaaatac aaccattttg tactctataa taaacattgc aagagctctt
     1621 gggatgctta cagtatgtga aggtgttgaa acagaacaac atgtagaatt tattaaaagt
     1681 ataaattgcg atatcgccca aggttattat tatgcaaaac ccatggagtg caaagaattt
     1741 gaaaaattac tatctaaaga gaataattca aagaaaatgt ttataaaata ttctgatata
     1801 ggaaaatctt taatagagaa gatatttgat ggatttttta ttatgcaaga tatgaaaaaa
     1861 ctaagagcta atgtcactga agacattcaa tggtttgatt tttacaacaa aactgaaatt
     1921 acatcttttc ctaaagtaat agaacatttt gaaaaacata ttaaaggtaa aaaattttct
     1981 attctttaca aaagtataac cccacataat actgaatcag attcattatt aattagtgga
     2041 gaagccgttt tatcagatga agcttctgaa catcaaaaat atagcaattt ctactttagt
     2101 gcaaattgta taatttcaga aaataaatta ctcttagcaa aattacattt ggatttaatt
     2161 aaaactgctg gctatataaa tgaatttgaa tcatattcaa tacctaaaat gacaaataca
     2221 catcttgatg aaagtaatat tttatcacaa ttttattcaa tgttgcctgt aggtataatt
     2281 cgctacgatt tatttggtga tatgattatt acttatatga atgaagaaat gtttgatatt
     2341 ataggttata caaaagaaca gttttttggt gagatgggcg gaaatttaag agcaattgtt
     2401 catccagatg atttagactt tgtttataaa aaaagtttgg atatgattga cacaaataaa
     2461 attgaaccat ttttatatag attcataaaa cgagatggtc aaattgtcac agttatgtat
     2521 acacaatgta acctttctgc aattgatgga aggtcgattg cccagggtat gtatgtagat
     2581 gtaaataaaa taaaaaattt aacaaaaatc tagtacaaag tattttattt tggagaaatt
     2641 aatatgttta aagaaatttt tttaagaact tttccaggat ttctaattat aagagattct
     2701 aactacagaa ttatatttat aaatgataat ttaaaaaatt taattaaaag ttatatgcaa
     2761 gataatccac ttggaatgac taatatagaa atagctaaga aactccctga taatattgct
     2821 aaatttttta cggattctca caatattcaa ttagattggg aaaaaaatta tccttatgat
     2881 aaaatttcaa attggatttt agagtttaaa aaagatacta caagctattg gaatgtttta
     2941 gaatataaag tagatgttga tgaaaaaacc tacataatta ctatggccaa tgatataaca
     3001 aaactctatg aagaaaataa gagaaatctt cactactcaa ttactgaccc attaactgga
     3061 gcatataata gaaaatattt aaatgataga tttgacatat ttataggaga ttatattgta
     3121 ctaattgact tagataattt caaaatgata aatgactatg aaggtcataa tgtaggagac
     3181 aagattttat gtgattttgt ttcactttta aataaagaat taataaattc tacttctatt
     3241 ataagattag gaggagatga atttattgtt atattctcta gtgatgttga caaaaattat
     3301 gtttattcac agctagaagc cttaagagaa aattttttaa aagtattctc taagtataag
     3361 tatctttcat ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt
     3421 gaattggata aaaaaatgta taaaaacaag gagaaaaaca agaaaaaatt tgataaaaat
     3481 gattattaat attgttattt taaaataaat taaaattatc tttctttttt taaacttgta
     3541 cattagttta taaatatgta tctatacaag atattgacat taagtactga agcatatatt
     3601 gtttcagttc ttttttgtta aaattaactt atttgacaaa gatatcatag tttaacaaag
     3661 cttttaattt aaatatattg atgtataata taattaaaat ataatagcaa aaagaaagga
     3721 ggaatatcat gagcatactc ctaaaaaaag cacccaaatt agcaaaacat attataacaa
     3781 gtttttatat aaatcgggat attgatgaag tattaaaata cctttgtgaa aatgttacat
     3841 ggattggacc aggagaacag gaatttctca cttcttttaa tgaaataaaa aattattttt
     3901 atgcaggtca agatgaaatt ccttcttgtg atataaataa tgatatattt gaaactgtaa
     3961 gtgaagatga gaatagatgt atggtattag gaagatatac tgttcgaact aaagaaaatg
     4021 ctcaaatgat tttagaggta aatcaacgtt gtacatttga gataattgag gatagaggaa
     4081 aattattaat aaagcacatg cacatatcaa acccttatgg tgaaatgcaa cttgatgaat
     4141 actttcccac taaaattgga actcaaagtt atgattattt gcaaagatta ctaaaagaaa
     4201 aaacagaagt tattgaaatg attacaaata atatcaatgg tggcttaaaa ggtagcaatg
     4261 atgatagtac atactcattt ttctatgtca atgagggatt gcccaaaata ttaggataca
     4321 catatgatga gttcatggaa atgagtggtg gttcagcagt gggtgctgta tatccaccag
     4381 acttacctaa agcactagaa gattgtgaac gatgttttgc aaaaggacct acatattcat
     4441 ctgaatatag aatccgaaaa aaagatggaa cattaatgtg ggtattagat tctggaatga
     4501 aatcgttaaa tagtgatggc atagtaaaaa ttaatagtat tattactgac ataacccaac
     4561 taaaaaacat agaatctgaa ctaaaattag aaagagagcg atatcgtatt gctttgcaaa
     4621 atattacaga tatcatgttt gagtatgata ttgaaaacga tacccttata aaatatcaac
     4681 gtgtagaaat tgacaaaaaa attgaattag aaaattttga aacaaaaaat tactccaaac
     4741 tattagaatc tggtaaaatt attcaccttg atgatattgg aaaactacta gaagtacttc
     4801 atggaaatct tcgtgaaaca attgagataa gacaagtaaa ttcattaact aaaaatgaat
     4861 ggagatggat tcgtgttcaa tgttcagtta tatatgatag tgaccataac cctatcaaaa
     4921 caataggcgt tttaaaagac ataactgaag ataaatctaa attagaaagc ttaattaatc
     4981 aggctcaacg tgacccacta acacaattat ataatcaaag agtatcacaa aatcttattg
     5041 aagagtatct atgtagttct gattccaaaa ataatgatgc tctattagtt attgatattg
     5101 atgattttaa aactgtaaat gatacttttg ggcatttaga gggaaatgaa gttttagttg
     5161 ctgtatcaaa aatactatta cataacacac atgataaaga catagttgct cgaattggtg
     5221 gagatgaatt tacaatattt ataaaatcat taacaaagga tttaataata aaaattacta
     5281 atgatatttt aaatgatgca agtaaaataa aagtaaagga caatcataaa ataacattaa
     5341 gtattggtat tgccttcact gatgattcaa ctaaattata taaagattta ttttctaaag
     5401 ctgataaagc tctttatcta tcaaaagcag atggcaaaaa ttgttatagt acatacgaat
     5461 aataaagcta tttatacgac caagaatgaa cctttaaatt taatttcata gtctaacaat
     5521 caaagaattg aagctaatct tatagctagt caactagttc ctagaaaaaa tttagacaat
     5581 atatttattg atactgctca tttttctaaa gagcaaattt atataaaaaa ttaaactttt
     5641 atgtacatac agatactaat gataaacatt tagcattagt atcttctatc ttaaaacaaa
     5701 atgataattt atctatataa aaaagtgctt tgttgacaga gttcactttt ttatataggt
     5761 acagaattta agtataaata catgttttat tctatacatt ttagttaatg taaaattcct
     5821 tgattgtact ctctaacttt tcaacattat actatttaca taccacttag ttaatataaa
     5881 actataaaag tgttgcaatt actgggtttt ataaagttaa tttacatacc acttagttaa
     5941 tataaaacaa gcatcaaata catttatcat tctgtctata attttattta cataccactt
     6001 agttaatata aaactaatac ctccttttat aaaaaaatgt cgtgaaaaag tatttacata
     6061 ccacttagtt aatataaaac atgtatttat ttgttcttgt actattgttt tactctattt
     6121 acataccact tagttaatat aaaacactcc aaaacttgat tctaaattta gaaaaggact
     6181 atatttacat accacttagt taatataaaa ctgtagaaag ctcgtttgtg gatgaagttc
     6241 taaaatcatt tacataccac ttagttaata taaaacggaa cttttaaaag attcaaggaa
     6301 aaaagattgg tttatttaca taccacttag ttaatataaa acttgtaagg ataagcctag
     6361 agagaatagc ccttctctct atttacatac cacttagtta atataaaaca ttattttttt
     6421 atcgttttga aacctgtttc ttctgcattt acataccact tagttaatat aaaactatct
     6481 tttgattatt ctgataaaaa gtaataatac aaatttacat accacttagt taatataaaa
     6541 cgctcccgaa gttgttaatg ttgttgcgac tcttgctgat ttacatacca cttagttaat
     6601 ataaaacatc aatactttga tgcagttttt aactagattt tcaatttaca taccacttag
     6661 ttaatataaa accccaaaat aaactgggca ttttcaatac ttacacatat gcaactctct
     6721 taaatttgca gtgaaccatg agtagtgcaa atgataaagc ttatcatata ccctcaacac
     6781 cttatatttc aagcgttaag ctctactttg taacaaatat cgctcactgc aaaactattt
     6841 acatttttat tatatcataa atatattatt tttgaatatc tataccaatt tgtggtataa
     6901 taaaagcaag aagaactaca atctatttag acgtagagtg aagttcaaat actaatgttt
     6961 attctttttg aacttgattt ttagttcaaa actaaagtca ctcctgcctg tgtgaactat
     7021 acccgctgtt ttatacagtt aaaacatgta aaaatagtta taaagtacta gtgtaactca
     7081 ttagtactct ttttattata ttcatataat taatatctat attttaaagt ttactatcta
     7141 aaaaagagca cgtcaaataa aaccctagat atttaatttt tttagagata cttaaaataa
     7201 attatgctct ctctgtactt aaatatttcc taaattcaaa tggagacata gaatttaatc
     7261 tattttgata tctaaaatta ttatagtagt atatataatc atctattgct ttaactaatt
     7321 attcatatgt atcaaatttc cttaaatagt acatttctac ctttaaaatt ccccaaaatc
     7381 cttccatagg agcattgtca aggcaacgac caactcttgt ataatatatg tatatctaac
     7441 accattaaga aatatatatt ttataatata tatttcttca tttaatgcac ttagaaaagc
     7501 aaagaaaata cagattagta cactttttag agaaaaataa aattgataca tctaaaatat
     7561 taaaaaatac tttagataat ctaaagacac atctcaaagt ttttaactaa attataaaac
     7621 taattacaaa tgctatgtaa cgataataaa taaaaaattt acttcgctta agcgacgtgt
     7681 cgttgaaata tttcatgttg ccaactggtt cgtaagttga taagaaaaga acactaatta
     7741 tttagagttc ttttcttagc tacaagtgga ttataatact tttaaatctg tgcgaaaatt
     7801 gtatataata atgatatgaa caagtaaaga aaatacaaaa atgacaaaat tataaataaa
     7861 atattataaa atcataaggt cataattgtt atattgtaga aacaaagtaa agagagatgg
     7921 tggagaataa atgaatcaaa aaaaaataaa gattagtatt aaaatggtaa ttataggtat
     7981 atttgctgta attatttctt ttgtattatc cagattattc gttgagtata ttgatgaatt
     8041 attaaggtct aattggcaat atcagctatt tgaaagtgag agttcatata taatatttgc
     8101 caattgtatt atgttctcga atatattatt agaaatattt ttaatatata tttgtagaaa
     8161 atttagaaaa atctaatata gtcaacgtat ttcgaatcga atacttcatg attatttagt
     8221 ctattgtgct agttaaagtt taattcccat ataaagtaaa ttgattttag taaaacaaat
     8281 cacccctaag ggttagttta tggataagtc atccaaatat taactcttta atttttgcta
     8341 tagattcatc tttgctcata actttactta taaaaaatga aatattataa gctagaactt
     8401 ttattctaat tctagtcata aatcctagca tagagttact ttttttatat tataaataaa
     8461 actatacaac aaagataata tttagtaaaa ataactttca ttttaactaa acttaaaatt
     8521 caattactct ctaaattgac gtttaggaat tagttcaaaa taaaaaagag tgtatatatt
     8581 gaaattacta catttcaata tatacactct ttttaaattt ctcagttttc catggaactt
     8641 tactttattt atttctgtat tagttgtatt ttttatgcat ttttctttaa aatcatattt
     8701 tctgattcaa ctcttatatt ataaaatttc tttactaaat tattctcttt ttctatatct
     8761 tcatatatat tttttaatcg ccttttacct aatcttttat ctaacattac taatgattta
     8821 actaatatat tatatgattt tatcgcatct tctactgaga tatttaaaca tatatttact
     8881 gctttataaa aatcaaattc actaaaaaat ccatttttat ttacaatctt atatcccttt
     8941 gcatctcttt ctaaatactc ttcctaaata ctcttcaaaa ctttcatcat ctaatctaaa
     9001 tcctatatca ctatgaaaaa taggttttgc atttggatat agttctattg ctttatcaaa
     9061 agtttcgaat accaagttat tattattcga ttttccaata gcccactata aacaataata
     9121 agtttgttgc tccaaataat gtaacacttg tagaaagatg ttacagatat ataaaagata
     9181 gagaatgtag tttttctcct aatactatta gaaatagaaa aaatatgatt aaaaatcaaa
     9241 ttgaagtttt ctttaaagat atgaaattaa tagatataac accttctatt ttacagtctt
     9301 atataaataa catatacaat gagcacatgt taaattcaac aaaaaaccaa gtcgatttta
     9361 taaaattagt attgaaagaa tcttatagat taaaagaaat atcagaaaat atttgtgatt
     9421 ttgttacaac cccaatcaaa aaaatagttt aacttctaag ctttatacaa aacaaaaagc
     9481 tcaattatta ttagaaaaat caaaaaatac tcctataggt ataccaattt ttttgatgct
     9541 aactcttgga ttacgttttg gtgaagctgt atcacttata tggtcagatg tagaccttga
     9601 taaaaaatct cacatgtaaa tcaaacatta gtttatgtat acaataaaat aatcttcaaa
     9661 ggccctaaaa cccctagatt caaaagaaga ttatttgttc ctgatgaact tataagtttg
     9721 cttaaagggg aaaaattaaa acaaaataag ttgaaattac agggtatatt gaaaaatgaa
     9781 tttgatttga tatgcttaga taaaagattc aacccgtgag ttcaaccaac ttttgttgtt
     9841 cattttaaaa aactattaag agataataat cctagagata taaaagtaca tgaattaata
     9901 catgcaaatg caactttgat attactttca ggtactaaca taaaagctat ttataaaata
     9961 ttagtacatg cagatataaa aatagctatg aataaatatt cgatattctc acttattaga
    10021 tgaaatggat aaagaaacat ctgaaaatat aagcaaaata ctattcaaat aatgttcagt
    10081 tttatgtcaa ttaaatgtca ctcaaagtgc tttttttcca tttgtcagtt aaatgtcact
    10141 taaagtacct ttttcctatt tgtcagttaa atgtcagtaa tatataaata agatatattt
    10201 ataagaccct taaaatcact attatttaat aaaatttata attatatact atttgttgtt
    10261 gacattggta ttataccata gtttatcatt tatattagaa ataaaattat taattaatag
    10321 tttcgtaatt ttataataga tttacaggga gtgataataa atggatacaa aaatatctat
    10381 aaacgaaaac atgagtctag gtaaaagatt ttttaaatac ctagcaccat ctgtagttgc
    10441 aatgtgggtt ttctcactat atacaatggt tgatggaata tttgtaagta aaggagttgg
    10501 agaattagct ttagctgctg tcaatatatc aatgcctttt attaatttta tatttgcagt
    10561 ttctctatta ttttcaactg gtgcatctac cataattgca atctatctgg ggaaaaaaga
    10621 tattaaatct gctaacgaag tcttttcatt taacttagta tcaataatta tattatcaat
    10681 aattatatta gctataactt tttttaattt agatagatta gcactatttt taggagctac
    10741 agaatctaca attggtatgg ttaaagatta tttaggaata ataatatttt ttaacggatt
    10801 ctttatagtg tcttattctt tagaagttat aataaaaact gatggctttc caatattagc
    10861 tactgttggt gtaataatat ctgctcttac aaacatcata cttgattact tgtttgtaat
    10921 tgaatttgga tggggtgtaa aaggtgctgg tattgcaact ggtttgtctc aagtcttttc
    10981 tactattttc ttcttaatac attttttaag aaaaaactct actttgaatt tttcaaaatt
    11041 tagaattgat tttaagacac ttagaaaaat tgtatttata ggttttcctg attcaactac
    11101 tgaattaagt tgtggtatag ttgttttact ttttaactta agtcttacta aatatattgg
    11161 agaaaatgct cttatttatt atagtgtcat aaattatata aatacattag tacttatgac
    11221 tatgatgggt ataactcaag gtatgcaacc acttactagc ttttattatg gtgcaggaaa
    11281 tatagataat gtaaaaaaat tattaaaaat ggggataaaa gccacaataa tagcatcagt
    11341 tgctgtattt gtaatttgta tggctttttc aggaccaata gtctctttat ttatacatcc
    11401 agaagaaaca atgctattca atgaaggagt tcgtgtattt aaaatattct ctatatcttt
    11461 cttattggta ggaattaatg taataatttc tggattcttt gtatcagtag aaaaaccatc
    11521 aatttctact gttatatctc ttggtagagg gcttgtaata gttgttttaa gtctaatttc
    11581 aatgatacta atatttggtg gacaaggaat atggatgact actattgttt ctgagtttat
    11641 atgtcttatt ttatcattag cttttctaaa aaagaatttt tctacattag actctaatct
    11701 aaataaggtt gcttagatga taagagtgac aatatatgaa aatatctaaa taaaaaatac
    11761 ctattataca atcttacaaa tattagacct ttgcaaaaat taggattata taataggtat
    11821 tttatttatg taattatttt atttctaaat atccatagcc atcttttatt aaagttcctt
    11881 ttacttcata ttctacaggt tttttaaata ttggaccatc tacgacaaat gtatgatact
    11941 ctccatgttc tccacatggg tctgcaccag cattttttat attctccaca agttctctag
    12001 ttaatttttt tcctaaaaat tcctctccta agtgctttaa gtttactttt gttacaacac
    12061 tacaaaatcc actgtctata aattcatata ctaaatcctc tctactctct tgccataatg
    12121 gtaaatctac tttcatacca actgcttcac atctatcagt atcccatttt ctatgacttt
    12181 ctatatctat atctccatat gcacatatcg ttgctcccat gtttttagct tttattaaag
    12241 ccctctcaaa gctctcttca tatccttcta cgccacactc agcaactaat aaaggtaatc
    12301 ctatgttgct acttacttgc ttaagcatat tatattctaa attatgtgtc catgactttc
    12361 catgttcttc acttatagtt gtcaagagtg ccacaggctt atatcctttt ttcaacattc
    12421 gatttaatgc taatatacta tccttaccac cactaaatga cattacaaat ttttcatcag
    12481 ccaattttaa cacctctttc atctaacttt gttttattaa cataattata ccaaattttt
    12541 gagttgcata gcaaattata ttaaattgtt aattcactat aaagaatgtc cctaatatta
    12601 aaagtataac ccctatgtat ttgtataaat gcattgtttc ttgtcccaaa attacgctat
    12661 caataaaaaa tccaactaaa acttgggata caagagttaa tataagtgta tttgatactc
    12721 ctaaatttgg aattgccttt actgtaaaat atattattag acctccaaat actcctccta
    12781 ataacaactt ataattaaaa ccatttatag atactagaca ctttaagtca cctgtaacta
    12841 ttatacttgc caaaaaaaat attgctccta cagttaagct tataaaggtg gccactaatg
    12901 ctgttgtact ttttcctagc tctccattta taaaagcttc aagagctgta gaaccacctg
    12961 ctaataatgc aaatattatt gcccataatt ttgtcatgga caaacctcct tctatataat
    13021 tctattaata gaagtttatg atacaaccta ttaattaatt actaagtata gtttaatttt
    13081 tatacactag gctttatttg gatatgaatt tctgttataa ttgttctatc gattttggag
    13141 gtgattttat gtacacaaat tttaatatcg attttaataa ctttaataaa aaagaaaatg
    13201 ctattaaatt tatgttaatg ggagtattac tcattatatt aggattatta tgccttacat
    13261 ttaagacatt aggtataaaa ttaatttctt ggacttttgg tattgcactt ttattctttg
    13321 catatttaaa tttaaaaaat attaatgagt taaaaagata tgctactaaa gaagaaatta
    13381 aaccctctat taatatccaa tgggttctaa taattgcatg tatattactt tttgtatttc
    13441 ctcaaaaaat acaaagcata ttttcattat ttttaggatt ttatttaatc tttaatcaac
    13501 tagtagcact tgtaaatagt aagaataatc cttattctaa atttacaact tggaatatag
    13561 ttaagattct atttggtata tgccttatat tatctccact ttttttatct agatttatag
    13621 tatcgataat gtcttttttc ataatattat ttggactagt tctatttttc tctggaaata
    13681 cagcgagaaa atattaatta ttttctcgct gtatttttat attacttaaa atagaatttg
    13741 tttaagttat ctatttgtgg taatatgttt tttgtacaag ctcagcgtac aattttatta
    13801 gaaataggtg attttttgga taaatttaaa tatgcttttg ataataaaag atatcatact
    13861 tggaattact atcttagaaa tacttttgga gaaaaagttt ttaaggtatc tataaatgca
    13921 ggatttactt gcccaaacat tgatggaagt cttggttatg gcggatgtac ctattgtagc
    13981 aaagaaggct caggtgattt tgctggaaac cctaaagata atcttatcag tcaattttat
    14041 aatataaaag aaatgatgtt aaaaaaatgg cctcatgcaa agtatattgg ctactttcaa
    14101 gcctatacaa atacgtatgc tcctcttgaa gttttaaaag aaaaatatga gactatatta
    14161 gaacttgaag atgtcatagg tctttcaata tccacaagac ctgactgttt gccagatgat
    14221 gttgtagaat acttatctga actaaataaa agaaccaatt tatgggttga acttggactt
    14281 caaacaattc atgattctac atctaaaatt ataaatagag gtcatgatta taaaacattt
    14341 gttgaaggtg ttgaaaaact aaagtctaaa aatataaagg ttgtcgtaca cattataaat
    14401 ggtcttcctg gtgaagatta taatatgatg gtggaaacag caaaggcagt tggtaaaatg
    14461 ggtgtagacg gtataaaaat ccatctattg catgttataa aagatactcc tatggaaaag
    14521 atgttacaaa atggtatgtt aactcttatg gaacaagatg aatatatcaa tcttgtctgt
    14581 gaccaattag aaatacttcc tgaaactatg ataatccata gactcactgg tgatggtaag
    14641 agggatgaac tagtaggtcc tatatggagc ctaaaaaaat gggaaatttt aaatcaaatt
    14701 gatgatactt taaaggctag aaattcttat cagggctgta agtttgttta atacaaaata
    14761 ataactatag aaatggttat ataaatatag tcctttctat agttatttag tatttacaaa
    14821 acaaaatatt aatttgtaca tttatacaat atataataaa aaatagatag tctaaactgt
    14881 atctacttat attttatttg tactgtccac tttattctat aaaatttata aatagttatt
    14941 ttttaatacc aaatataatt tggtataatt tttgttggac tctaaaatgt ataccaataa
    15001 atccttttta agtatataat tctaaatact ttcagttttt tcaatctgct ttaacttgtt
    15061 ggacttataa aatattctta tttcatcata agaaagatta agtgctacta ctgcagatat
    15121 taacatcaac atcactccaa ataacttccc taaggaaaaa ctttctccta atatagtgca
    15181 accaattatt actgaaccaa ctaattctac tgatgccaca acacctgcaa ctgacaactc
    15241 tacaccttta gatatacctg ctgcataaaa aatatatgca agtgcagctg gaacaatgcc
    15301 taacattagc atacaagata aaacatctaa atccttagca taatttaaga tttcccaagg
    15361 tcttgaagaa ggaatcataa atatagctcc aaataagaaa ctgtatacta atattgttga
    15421 acttgaaaat tcctttaaag catttttact aatagttggc ataagtgcat atgctattgc
    15481 tgataatacc ccaagtaaaa gtccaaaagc gtttaaacct tgaaaatcca aacgtccacc
    15541 agtcactgcc ataattgcac caataaaaca aagaataagt gaaaataatt tgcttctagt
    15601 aatattttct ttataacata tttttgaaaa tattgctaaa aacagtggtg atgtatatag
    15661 taaaactgct gcaattgata cacccgcaat gtcaattgct ttaaaataac ataaattaaa
    15721 cattgcctga catataatac ctattattac tgaatatata attccttttt tgcttatctt
    15781 taagatttgt ggtgtcctta tagatgaata tacgcctaaa actataaatc caaaaaacaa
    15841 tcttgtaaat gctacttgtt ctgatgtcag tccatttccc attaaaaact ttccaaataa
    15901 tccaagtgta gcccataata accctgctat tgctataaat acataccctt tacttttttc
    15961 tcccatttta aattcccttc tatttttatt tagtcaattt acactcttct ttttaagcaa
    16021 aaactatgcc atcttaaatc aatatatatc gactctaaaa ccctagtctt tccaataata
    16081 ctaataaatt ttcaattttt ttatatttat ttttcactta ctttaagttg atttagactt
    16141 attttaattg taaaatgagt tatatataac ttatttaaag ttaaaataga ctgaaactta
    16201 tatataaata tcaaatttta agtatattaa tcaattaaac ctattttaat atatcattaa
    16261 aaatttagga ggaaaatata aaaatgaatt tagatttgga attttatcaa aaaatattag
    16321 aagcttccca tgatgaaata tgtgtttctg atgataaagg aataataata tattgtaata
    16381 aagcatttga agaaaattat ggtctaaaaa aagaagatat tttaggaaaa aatgtttctt
    16441 ttttagaaga ctctggatat tcaacaaaaa gccccatacc aattgtatta aaaacaaaga
    16501 ctaaattttc actggaacaa gatactcaaa cagggaaaaa gcttatcata actgctaccc
    16561 ctatatttga tgaaaatgga aacttagaat ttactgttga aaattgtaga gatataactg
    16621 aacttaataa tataaaaaat aaacttgaag acacaaaaaa gcaagtaaaa aaatataaat
    16681 ctgaagtaga aacactatac agaactgcac ttagaattga ggatactgta attatggacg
    16741 gaatagttat gagaccaatc ataaacacag taaatcatgt ttctaaaacg gatgtaagtg
    16801 tacttttatt gggtgaatct ggaacaggaa aaagctcact tgctagatat atacaccaca
    16861 acagcaatag atctaatggc ccatttataa caataaattg tgcaactata tctccacaac
    16921 ttcttgaatc tgaacttttt ggatatactt caggtgcttt tacaggtgca agtactaaag
    16981 gtaaagttgg gctcgtagaa ttagctaatg gaggaactct ttttttagat gaaataggag
    17041 atataccaca aaatctacag gcaaaatttt tgcaattaat acaggataga acttttactc
    17101 cagttgggag cttaaaaaat aaaaaggtcg atataagaat tatatctgct acaaatgttg
    17161 atttagtttc aaaagtaaaa gaaaaaaagt ttagagaaga tttatactat agattaaatg
    17221 ttatagaaat aaaattgcct cctcttagag aaagaaggga taatctagtg gaaattataa
    17281 aatattattt taataggtac tcaagtgact tcaatctaaa taaaactatt tctaaagaag
    17341 ctatggatgc tattgcaaat tatagattcc caggcaatat aagagaatta caaaatataa
    17401 ttcaaaaaat cttgcttact tgtacagata atcatataac aatagatgac ttaccaaaca
    17461 ttcttacaaa aaatatacat attaccaata atggaaataa aacacatata tcccaaatta
    17521 ataaggttat tatatctgac tcaaaatcta ctaattataa aaataaaaat tttgacactt
    17581 taattaaaga gtatgaaaaa aatataattt tggatgctta tgaaaaattt ggtagttctt
    17641 ataaagtagc caaacacctt gaaatctcac agtctaaagc caataggtta attagaaagt
    17701 atactaatac ttaattttaa atttgataaa taaaaactta acactattta catattgtaa
    17761 atttggccat ttactgttat tatgaatatg gttgaaatta tttaaataaa tactgttgac
    17821 ttagtttcat atatttacca ttaaactttt atcataaata tcatatcata ataatatatt
    17881 taataagtct aataaactat acgaggtgta aatatgttaa tgaataccca ttttattatg
    17941 gcaaaatcaa tacttgataa tatagatgaa aataaaacat tctttataag cgaaaaaaat
    18001 tttatatatg gcaatataaa gcctgatgct ttttctaaat ataaattaaa aaaacattat
    18061 ttagatgagt catttaatat gattgtttct aaaataaact atctatgcag tcttaatata
    18121 gatagtcttt ctaaatactt ttcagtaagt agacttagtc aggaactagg agtcatatgt
    18181 cattttttat gtgatttttt ctgtgtagct cacagtgaac gttgggagtt taagcatagc
    18241 ttaaataaac atgttactta tgaaagagaa cttggagcat ttgcaaaaga tgttgattta
    18301 tctaaaatta aatgtggtac tataaatagt agttttgaga agttttttac taaactttat
    18361 agtgattaca aaaagaaaac tgattatatg aatgatttaa aattttcagc ttatacttgt
    18421 aatagtgtca taaattatat acttgactct attttatcta atacaataaa atcatatcat
    18481 atagcaaatt gcggttaact taagaatttt taaacttata taaacttata attgaatata
    18541 attaaaatga ctgctctata gaatataatt ttatacttct ctaggacagt cattttttat
    18601 actaactatt tatctccatt ataacatcta tggcactttt tacactatct actacataat
    18661 ttgcactttg ctttacatct tcccttgcct tagacattgc aaaactatat ttaaatcctt
    18721 ctaacatgca aatatcattt ggagaatccc caatagtagc aacttcatct ggacttacat
    18781 ttaacgtttt tattacatgc tctatacttc ctctttttga cacactttta ggaactatat
    18841 ttaaagtata tgttccagaa aaatatattt ctaagacttc actaaactca ctttttagat
    18901 aaagttctat tctggataac tcttccaaat tttcagaaca tacgcctata tttcctataa
    18961 gattatcaaa actacttctt ttttcaaaat ctgggtcaac tataaagtca ctagaataat
    19021 gtctaaaagc agtggtatca ctacccacaa ttacatcacc cttatattta aaataaagtt
    19081 gtgcatcact tgactcaaat ctatcaatga ctttttttac taaattatca tctatcgttc
    19141 ttttaaaaat tacattataa tctttatcat atataaatga accattttca catacataat
    19201 atccattcat atctaaacta tctttgataa tattatgtgc ttgtttaaaa attctacctg
    19261 tagcaacatt aaactgtact cccttttttt ctatttcttc aattgctact atatcttctt
    19321 ttgttatggt tccattttca tatagagttc catctaaatc acaaaatata tgttttatca
    19381 taaattaaaa gcctccaaaa acaattttat attttactaa tactttaatc aaaataactt
    19441 aaagcttttc taattgtatt ctcaactcat taagcaaatc tctagtagct cttaatctta
    19501 tctttcttct atccccatta aatatatttt tctttactat tgtttttcca tttatatata
    19561 ttgcagtata aaccagtccc acaggcttct ctttggttcc tccaccaggt ccagctactc
    19621 cagttgtaga tatacctata ttagctttaa gtcctttagc aacgccctca gccatttcta
    19681 tagctgtttg ctcacttact gctccaaact cttccaaagt ttctcttttt actcctaatc
    19741 ttttcatttt agaatcatta gaatatgtta cacaaccttc aagaaataca gaggatatac
    19801 ctggatagtt tataagagat gaagaaacca aacctccagt acaagattca gccactgcta
    19861 ttgttagatt tttttctaca agtagcttag aaacagcatc ttctaaagca gtctcccctt
    19921 cagatatgga aatatctcct tcagtatata catatttacc aactctactt tttatttttt
    19981 cttctactgg ttttattaat ttaaaagctt ctctttcatt ttcagccttt gcagttattc
    20041 ttattgtaac ttctaattct tttgcatata aagcaactgt tggattagtc tgctcattaa
    20101 ttatatcgag aatttctaat tctagatttg actcacctat tccatataat cttaaagtct
    20161 tagatactag catttcattt gtgaattgtt gtaagtaagg tttgacactc tcattaaaca
    20221 tagctttcat ttctcttgga ggtccaggca atactattat aaatttacca tcttttttta
    20281 aaattgcccc tggagctgta ccattattat tttttagtac tatagcatct tttggaaagt
    20341 acacttgttt catattattt tcggccattt ttttccctgt tttaacaaag aaagattcca
    20401 aaacctctag tgaaggttta tgtagttcta atttctgacc aaagtattct gcagcagttt
    20461 cttttgtcat atcatcacca gttggcccaa gaccacctgt agtaattaca aaatcacttc
    20521 tctttaagct ttcatcaaaa cattctaaaa gtctttgttt gttatctcca acagtactct
    20581 gatggtatac ttctattccc aatgatgcaa gttctttagc taaaaattga gaatttgtat
    20641 ttactatatc tcccaataat atttctgttc ctacacttat tatttcagct ttcaaaattt
    20701 atcactcctt gatttatagc cattaagact agtcttaagt tcataacaat aattttagca
    20761 aaaataataa cataaataaa gttaaaaaat catttaaaca tatctttaaa ctagattatt
    20821 attctaaaac cacacataat ataaacttcg tatatagtgg tactttcatc ctcaactgtt
    20881 aattcatata attaagtatg taaataaaaa gtttacttta aaataataaa aattaaaata
    20941 ggagtgtata atatgaaaat agcagttata atgggtggaa tatcatcaga gagagaagtt
    21001 tctttaaatt caggaaaaga gatatataat aaccttgata aaaataaata tgaagttgta
    21061 aaagtaataa tagatgataa aaaagatata tttacaaaga taccagaaga tatagatttt
    21121 gcaatacttg ctttacatgg aaaatttggt gaagatggat gtatacaatc aatacttgag
    21181 actatggata taccatactc tggatgtgga cctctttgca gtgggatgtg tatggataaa
    21241 aatataacta aaaaaatgct tagagattca aacctaccta ctgctccatg ggttttagta
    21301 aaatcagttg atgaaataga ctatgatgaa atagataata taggttatcc agtatttata
    21361 aaaccaaact caggaggttc aagtgtagcc acattcttta ttcattctaa ggatgaagtt
    21421 gaagaagcag ttagaaaagg tctggaagtt gatgaatttg taatgattga aaaatatata
    21481 ccaggtggag aatatacttc attcatatta aatggagaag tattccctac aatatctata
    21541 aaatctgatt caggattctt tgattatgaa gctaaatact cagttgaaaa aggtgcaaag
    21601 gaagaagttg tttacttaga tgaagaactt caaaaaagag taaacgaaat ttctgagact
    21661 tgttggaaaa tttttaattg caaagcatat gtaagagttg atatgattat aagtgaaggt
    21721 attccatatg ttcttgaact aaatactctt cctggaatga ctcaaactag tttaattcct
    21781 agaagtgctg ctgctagagg tattaagtac agtgaactat tagataaact tatagaatac
    21841 tctttaaatt agatgaaata atttgaggtg gtaatttgat attttcaaat ctaaaattaa
    21901 atgataatga accaatttat atacaactta aaaattatat aagtgatatg atatcaaaag
    21961 gactaattcc agacaatagt aagctccctt ctacaaggga gcttagtcag cttttacaag
    22021 ttagccgaaa ttctgtagtt ttaacttatg aagaattaaa atcagaagga ttaatctact
    22081 ctatttcagg aaaaggtaca tttgttaaat caaagaataa gtcttcaaat actacatggt
    22141 cattaaactg ggattgttta gagaatacat actctaaaaa agccaatgaa ttggacatta
    22201 taaaaagtga aattccttgg agctctgacc tgatttcctt taaaagtatt tctccagatg
    22261 gagatttatt cgacatggaa gaattaaaaa aatccttttt aaatagaatt tcactagaag
    22321 gacataaatt actcaactat ggatatgcac aaggttataa gccacttatt gactatttac
    22381 tagaatatat gacaaacaag ggtgctgata taagtaataa agatatacta ataacaaatg
    22441 gattcacaga aggctttgat attattatgt catcttttac ccaagaaaaa gactacataa
    22501 tatgtgaaaa tcctactcat aatactgcta taaaaattat gaaatctcat aacctcaata
    22561 tcttaggtgt agatataaat gaagatggtt tggacttcaa tatgctagaa gacaatttag
    22621 ttaagtataa aaataaaata aagtttgcat acattacacc ctcttatcac aatcctacag
    22681 gaattgtaat gactcctgaa aataggtaca aattctataa tcttatgaaa aaatacaata
    22741 tagcaactat agaagatgga tttaatgaag aattactcta caatagttct catatatttc
    22801 ctatctgttc cctagataat atgaataatg gggtcgtata tattggtagt ttttcaaaga
    22861 ttttatttcc tggaatgaga attggatgga tatttgcaga taaaaaagta atcaataagc
    22921 tagaaagtgt aaaaaggtgc agaaacatac atgtatcctt tttagaccaa ggaattttat
    22981 ttgattattt aagtaatgga ggttttgaaa aatacattaa aaaaatacgt aaattctatg
    23041 gagacaaatt taactttgca tataaatgta taaaaaaata tataaaaaca gaatatatct
    23101 tgggtgatgg tggtttgcat atatttataa aactaaaaaa tatagatact agagttttat
    23161 tagaaaaatg ttatgaaaaa aatgttatat ttatgcctgg agacttattt tttacagata
    23221 actctggttt tgatacttta agactcggat tttctaggtt gtcttttgaa gacattgaaa
    23281 ttggaataaa aataattgga gaaactataa atgaaatgtt ctaggttaac taaatattgt
    23341 tagcctagtt tatttttatg aggtataata tatatagttt atatgaatct aatactgggg
    23401 gataatatga gtaaaaagtc aaaacgaaga aaaattaata aattaaaatt aacgattgta
    23461 ataactgtat tatttcttgc tttattatct atatatgaga tactgttctc taatcccaac
    23521 aaaaaaggta atataaagag tcaagaaact atgcaaaata aaaataatac aacagatgat
    23581 tctaaaaata atcaaagctc taccaaaaag caggaagata ataatatcaa gcctactttt
    23641 tcatattctt caataccaga taatataaaa aataaaatga ttggtaaatc tatgcctact
    23701 gatgaaccta taagctttga tagtctatct tatttaaaac ttacatatta tggatttgat
    23761 gaaaaaactc atcaaggaga aatgatagta aacagtgaac tagctccaga agtagtggat
    23821 atttttaagg aattatacga aaaaaaatat ccaattgaaa aaattaagtt aatagatgac
    23881 tatgatgctg tagatgaaaa atctatgtct gataataata cctcatcatt ttgttataga
    23941 actatagctg gtacaaatgt agtttcaaat catggtaaag gacgtgctat agatataaac
    24001 ccattacaga atcctcaagt ttctggaaat gatgttacac ctaaagtaag tacagtttat
    24061 gctgataggt ctagcacaaa atttggaatg ataaaaaaag gtgatgattg ttataatgct
    24121 tttgtaagca gaggttggag ttggggtggc tattggaaaa acccagatta tcaacatttt
    24181 gaaaaataat agattagaaa taaaggtgaa ttatatgaat ttaatgacat tggaaaatat
    24241 aagtaaaagc tattctgaaa aaaaattact agaaaatata tctcttggaa taaatgaagg
    24301 ggaaaaaata ggacttattg gtgtaaatgg cactggtaaa tctaccttac tcaaaattat
    24361 tgcaggagtt gaagaaagtg aaactggtaa tataacaaaa actaatggta ttaggataga
    24421 atatctacct caaaatccaa cttacaatga aaattcaact gtacttgaac aagtatttaa
    24481 tggaacttct gaagaattaa agctacttgg tgaatatcaa gaaatcctag aaaaacttaa
    24541 ttcaaacttt acagaggaat taaataaaaa attattatca gtacacgaaa aaatagatgc
    24601 cttaaatctt tgggatttag aaagtgaagc taagtcagta cttacaaagc ttggtataaa
    24661 tgactttaat caaaaaatag cagaactttc tggtgggcaa agaaagagag tctctctggc
    24721 atcagccctt attacacctt gtgagttttt gatactagat gagcctacaa accatctaga
    24781 caatgatact atagattggc ttgaggaata tcttaattcc agaaaaggtt cattgcttat
    24841 gataactcat gatagatact ttttagaccg tgttacaaat agaattattg aactggacaa
    24901 gggtagatta ttcagttatg atgggaatta ttcaatattt ttggaaaaga aaatggaaag
    24961 actttcgcta gaagcaagta ttgaagataa aagacaaaat ttaataagaa cagagcttgc
    25021 gtgggtaaga cgaggagctc aggctcgtag cactaaacaa aaagctcgtt tacaaagatt
    25081 tgatgaactt ataaatagag attcttttaa acctgatgaa aatcttgata tatctgttgc
    25141 atcaagtaga cttggaaata aaataataga aatacacaat atatctaaat catttgaaga
    25201 aaatactgtt atagataatt tagaatacac tttaactcgt acagatagaa ttggtataat
    25261 aggcaaaaat ggtatgggta aatctaccct tataaatata cttaatggag aaatagagcc
    25321 tgatagtgga tatatttcaa ttggagaaac ggttaaaata ggttgttttt ctcaagacga
    25381 ctctcatatg aatattgata tgagggctat agactatgta aaagaagcta gtgactatat
    25441 agaaacttca gatggtacta ggattacagc ttctcaaatg tgtgaaagat tccttttcaa
    25501 tggtactatg caatatactt tgataggtaa attatctggt ggtgaaagaa gaagactaca
    25561 tcttcttaaa atccttatga cagctcctaa tgtgcttctt ttagatgaac ctacaaatga
    25621 tttagatatt gaaacgctta aaatattaga agaataccta gacgaattta aaggtgtagt
    25681 agttacagta tcacatgata gatatttctt agatagaatt tgtaataaaa tatttgcata
    25741 tgaaggtaat ggaaaaattc atatctatac aggtaattac agtgattatc ttatttatag
    25801 agagattcaa ggaattgaat ttgaagaatc taaaagggaa tcagttaaaa aagatgactc
    25861 tactacccta aaaaaagaaa aacctaaaaa tgataaaaag cttaagttct catataatga
    25921 acaaagggag tttgaaaata ttgactctga tatagaaaaa ctcgaatcta aaattgcaac
    25981 acttgatgaa agtactgcaa aattttcaac tgattttact aaactccaag aaattttaga
    26041 tgagaaatct aaactagaaa aagaacttga atacaaatat gaaagatggg aatatttaaa
    26101 taatctagct gatgaaatag ctaataataa gtgatattaa aataatctta agtttaataa
    26161 gttttgcttt aaatctgtaa tttataaata ttttaaagga agtgtctcaa aatgaacttt
    26221 ttagttttat gagacactct tttttatgag aaatcaaata tattttcatc atttcaacaa
    26281 tacaaaagag gcttatctct attttgagac agcctctttc ataattacct tctaatttat
    26341 atgctctaat atatctataa taattcttat tatatctata tatccagtct ttcaatatag
    26401 tgacaattaa cttcaatatg atttaaaaat actaacaacc tttcataaag tggatgagat
    26461 tcttctccat atttagcttc taggttcttg cctatttctc taacagtcct ttgaccatca
    26521 atttgtaaaa aaacaaaact tgaatattca tctagttcta tttttttgta tttaggtatt
    26581 ctagctttta actttctaaa aaacttttga attttatggt cctgtttttc aagtatagta
    26641 acaacactat tttcagctac ttcatattca agtccatcag taattttaaa tacaatatct
    26701 aaaacatcat tactcttgct cattattttt tactttagat aaaacaatag gtaatgctgt
    26761 aagcacaact aaaactacta ataaagcaat tgccattgaa tttgttgcag caaatccact
    26821 tggtaccttt ggagttacaa ctccagttac ttgtaatatt atacctatta aaccaatgat
    26881 agaaccacca gcaacaagac cagaagataa acttatacca ttagaaactt taacttcttt
    26941 ttctttttca gttttagata ctaattcaac aaataaacga attaatgctc caactaatat
    27001 tatagaagtt gttgcaattg gtaaatagaa tcctatagct atagtcatta ttggtaaatt
    27061 taatgcatat agaacgattg ccataaatac tccaacaata atcataaccc aaggtaaact
    27121 acctgacata ataccagaag ttaatgttga cattaagttt gcttgtggta atgcaaattg
    27181 tacattatca ccagttgctc taagttggtc agaaagtaat aaaattactc ctacaacaac
    27241 tataacacca actatacttg caattgtaaa gtaacgttgc atttcgtttt tgctaccacc
    27301 aataatataa gttacttttt gagattgagt ataacctcca gctatagcaa tagcaacaac
    27361 tataaatgaa ccaaataaaa gtaatgattt attagcttct aaatcagtcc atcccattat
    27421 aacaaacact aatgttacaa taactagaga agctatagtc ataccagaaa ctggaagatt
    27481 tgatgttccg attgttcctg ttaaacgacc agctactatg acaaataata aagataataa
    27541 aagagatatg atagctccaa taattgccat aacaatgtta ccagaaatta agaatgctgc
    27601 taaaaatcca ataactactc cacctaataa aagtatcatt tgtatagatg aaccttcttc
    27661 tccttctcca gcacttgatt tagctttaag tgtttctttt atagaagcga ttatagttgg
    27721 tataagtttt attgcaccaa taatacctcc acaaagcatc ataccagcac ctatatattt
    27781 tacataacta ctagatattg ctccaacatc catttggtta agaggcattg caggattatt
    27841 ccataccata gctccatctt tagccatatc tgtaaagtat cctattaaag gtgctatacc
    27901 aaagtttgat aaaatacttc ctgcaaacat tgttaaagaa acttcaagcc ctactataaa
    27961 tccaattcct aaaagtagag gatttacttc aatatcaaat ttccatttgt agaatttgct
    28021 tcctacaaga ctcattacat tgtttgctac atttaagaat gaaccagtta atacagtaat
    28081 aaatccactt attacaaatc caattcccat gtacttaatt gaatctccac cagcctctga
    28141 agcaacaagt gtctcagata tagccattga ttctgggtac ataagtttac catgttcttc
    28201 aactattaga tagttgtgaa ctatagcagc aattcctata ccaaataaaa ctccaccaac
    28261 tccaacagct aagccttcta agaatgtaat ttggcttcca attaatataa ctgctggtaa
    28321 tacgaaaata aatccacttg caacagattc tccaccactt gacatacctt ggattaagtt
    28381 tttaccaaga ataccctttg aacgagcaaa tgctccaaca aaagcagaac ctattatagc
    28441 tcctggaata cctgcggcaa ctgtaagacc ggctttcatt ccagagtaag ttgtagatgc
    28501 ggcaaaaatg gcagctaaaa taataccgat aatcagtaca gctacatttc caccggttct
    28561 ggacttatct gtgatatatg gaacgtaatc ttttccacta acctcaccat aagcaccctt
    28621 aggtaatttt ttgttcataa aattttcacc ccttttcaat ttgtatatat tataacatat
    28681 atacatataa aacttaactt tttattattt tacaaatagc gagtcaaaac tactaaaatt
    28741 taaccctata ttattaattt ctacttttag atatttttat gctaattttc tttttatttt
    28801 tcaattctac taaaaactat taagatataa aataaaaaaa ttttttaatg taaaattaat
    28861 tttgtaaaaa aaatgcttta aaacacggaa aagtataaaa aatacaataa tcattcttat
    28921 aaataaattt tcaccatagt aaatatatac agaaaattct tgtttttatg caaattgatt
    28981 cttttataaa aataagtgtc ataaagaaat gtctgaaatc actttatgac acctatcata
    29041 ataaaaaatt attctggtaa tgctctttta tttacttttc cgattggtgt aactggtatc
    29101 ttatctatcc tcattattat tgttggaatc atatatggag gtagatgctc atttaaatat
    29161 tctctaatag ttctttcatt tatcttacca ctatcttcct catcaattac ataatatgca
    29221 catatataca tatctccatc atcatttgtt ctcgatatac aaacaacact tttaatttct
    29281 gtaatattat ttattatatt ttcaatctca tatagctcta tcctaaactc atcaattttt
    29341 acttgattgt ccatccttcc aatataatct aaatttccat catctaacca tcttactaaa
    29401 tcaccagttc tatacattaa cttattatgt tcacaagttt tgtaaggatt ctctacaaat
    29461 acttgctctg ttagcccttt tttatttaaa tatcctcttg atatttgaat gcctgataga
    29521 catagctctc ctggtgctcc aattggacat agtttgttct ttttatctaa tacatatgca
    29581 taagtattaa atataggttt tcctattggt atttttccat attctctgtc cacatgaaaa
    29641 gaagtcgata caacagtaaa ttcggttggt ccatactcat tatatagttc ataattacgc
    29701 tttctatatc cttttaatct ttctcctcca gtctgtacaa ctctaagtga attattattg
    29761 caattttgta taaataacct tgcaaatgct gttggcaatg ctagtatagt tatgtcattt
    29821 tcctcacagt attcatttac tgtatattca tcaaatttag cttcttcagg cataatatct
    29881 attgatgcac ctacaagcaa ataagggaat agttgtttca ttacagcatc aaaactgaaa
    29941 cttaagtata taccacatat atcattttct gtagcgctta tagattttac gctataataa
    30001 cacatattta ctaaactctt atgttctata gctatggtct ttggcctacc agttgaccct
    30061 gaagtagata tcgtatatgc acaatttgat gatgttattt tttcagttaa ttcatatcca
    30121 agcttaaaca tttcttcatc atttatatcg agtactatgg tattctcatt tacaacctta
    30181 cttttgagtt catctgtagt tatcactact tttgcattac tatcttcaag aaggtaattc
    30241 aatctttctt ctggattact tgtgtctatt ggaaaaaatg ctccccctga tttcataatc
    30301 cctattgctg taataattat gttaatatct cttggtaaca ttacagctac tatgtcttca
    30361 ctttccactc ctatatgatt taaataacct gcaagtttat cagttacttc atccaattct
    30421 ttatatgtta actttctatt ttcatcccta gcagctatat tactaggtgt taccttaact
    30481 tgctccctaa ataaatcaac ataactttta tcatagggta cttcatattg attattatta
    30541 aagtcttcca ataatacttt ttcttcttcc ttacatacaa attctaaatc ttctattaaa
    30601 atatcattat cttctattat ttgctctatt atataatcaa attgatttaa aattcttcta
    30661 atagtttctc ttttatatat agatggttta tactttattt ttattttaag tgccctctta
    30721 gtatatacta ttacagataa atcatatcct tttttttcta tgtcataatc atatgataaa
    30781 tcaaaacttt tagctataaa tattgtgttt ataagcttag tatttagatg atttttattc
    30841 tctatatctg ataacaaata gtatccatac tctaaagacc tgctccactg cctttttagc
    30901 ttccttacaa catctataaa tttttcactt tgtctaactc ttactcttat tggaattata
    30961 ttcattgttt taccaaaaac tacatcatct tcatatatat acttttgaag tataaggcca
    31021 tatatagttt ctatgataga gtctatgcta atattgttca ctttagaata ttgaattaac
    31081 ctttcatgtt tctcaatatt catcttaaaa acctcatcta ctacttcatc agaacaatct
    31141 ttataaaaaa aatcatttgg aatttttgct atattcgtat aatcatctaa caaatccatc
    31201 caataattca tagctcgttc taaattaact tcatacatat caaaattccc ccttactact
    31261 aagtctatag aaaaaattct tttggaattt tattttcaca atatataaat atattaataa
    31321 tacatatgca agctctaaaa acatgagctt gcataaaatt acacttatta tgactactaa
    31381 attcgtaata tttacaatgt ttaaaatata ttataacaca tatatgattt tatttttc
//
LOCUS       DAENCV010000039        30661 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAENCV010000039 DAENCV010000000
VERSION     DAENCV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.039"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            126..2027
                     /locus_tag="KQD82_001768"
     CDS             126..2027
                     /locus_tag="KQD82_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4380242.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2030..3073
                     /gene="ypdE"
                     /locus_tag="KQD82_001769"
     CDS             2030..3073
                     /gene="ypdE"
                     /locus_tag="KQD82_001769"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG4380243.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3088..3393
                     /locus_tag="KQD82_001770"
     CDS             3088..3393
                     /locus_tag="KQD82_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4380244.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3490..3825
                     /locus_tag="KQD82_001771"
     CDS             3490..3825
                     /locus_tag="KQD82_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380245.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3857..5179
                     /locus_tag="KQD82_001772"
     CDS             3857..5179
                     /locus_tag="KQD82_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380246.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5189..6259
                     /locus_tag="KQD82_001773"
     CDS             5189..6259
                     /locus_tag="KQD82_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4380247.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6264..7451
                     /locus_tag="KQD82_001774"
     CDS             6264..7451
                     /locus_tag="KQD82_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4380248.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7648..8520)
                     /locus_tag="KQD82_001775"
     CDS             complement(7648..8520)
                     /locus_tag="KQD82_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG4380249.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNVLWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8914..9105)
                     /locus_tag="KQD82_001776"
     CDS             complement(8914..9105)
                     /locus_tag="KQD82_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380250.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9325..10371
                     /gene="cobT"
                     /locus_tag="KQD82_001777"
     CDS             9325..10371
                     /gene="cobT"
                     /locus_tag="KQD82_001777"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG4380251.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10403..10963
                     /gene="cobU"
                     /locus_tag="KQD82_001778"
     CDS             10403..10963
                     /gene="cobU"
                     /locus_tag="KQD82_001778"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG4380252.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10973..11746
                     /gene="cobS"
                     /locus_tag="KQD82_001779"
     CDS             10973..11746
                     /gene="cobS"
                     /locus_tag="KQD82_001779"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG4380253.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11766..12380
                     /locus_tag="KQD82_001780"
     CDS             11766..12380
                     /locus_tag="KQD82_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG4380254.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12447..12626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12756..14255
                     /locus_tag="KQD82_001781"
     CDS             12756..14255
                     /locus_tag="KQD82_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG4380255.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSSLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14294..15670
                     /locus_tag="KQD82_001782"
     CDS             14294..15670
                     /locus_tag="KQD82_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG4380256.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15671..16642
                     /locus_tag="KQD82_001783"
     CDS             15671..16642
                     /locus_tag="KQD82_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG4380257.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16674..17744
                     /locus_tag="KQD82_001784"
     CDS             16674..17744
                     /locus_tag="KQD82_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4380258.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17746..18633
                     /locus_tag="KQD82_001785"
     CDS             17746..18633
                     /locus_tag="KQD82_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG4380259.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18621..19253
                     /locus_tag="KQD82_001786"
     CDS             18621..19253
                     /locus_tag="KQD82_001786"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG4380260.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19262..20488
                     /locus_tag="KQD82_001787"
     CDS             19262..20488
                     /locus_tag="KQD82_001787"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG4380261.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20485..21093
                     /locus_tag="KQD82_001788"
     CDS             20485..21093
                     /locus_tag="KQD82_001788"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG4380262.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            21083..21658
                     /locus_tag="KQD82_001789"
     CDS             21083..21658
                     /locus_tag="KQD82_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG4380263.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21651..22403
                     /locus_tag="KQD82_001790"
     CDS             21651..22403
                     /locus_tag="KQD82_001790"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG4380264.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22387..23508
                     /gene="cbiG"
                     /locus_tag="KQD82_001791"
     CDS             22387..23508
                     /gene="cbiG"
                     /locus_tag="KQD82_001791"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG4380265.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23505..24230
                     /gene="cobJ"
                     /locus_tag="KQD82_001792"
     CDS             23505..24230
                     /gene="cobJ"
                     /locus_tag="KQD82_001792"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG4380266.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKSGDTPVGVVKDVGREKEEKLVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24227..24979
                     /gene="cobK"
                     /locus_tag="KQD82_001793"
     CDS             24227..24979
                     /gene="cobK"
                     /locus_tag="KQD82_001793"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG4380267.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24998..26476
                     /locus_tag="KQD82_001794"
     CDS             24998..26476
                     /locus_tag="KQD82_001794"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG4380268.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26506..27411
                     /gene="hemC"
                     /locus_tag="KQD82_001795"
     CDS             26506..27411
                     /gene="hemC"
                     /locus_tag="KQD82_001795"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG4380269.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27401..28900
                     /gene="cobA"
                     /locus_tag="KQD82_001796"
     CDS             27401..28900
                     /gene="cobA"
                     /locus_tag="KQD82_001796"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG4380270.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28917..29882
                     /gene="hemB"
                     /locus_tag="KQD82_001797"
     CDS             28917..29882
                     /gene="hemB"
                     /locus_tag="KQD82_001797"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG4380271.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30035..30613
                     /locus_tag="KQD82_001798"
     CDS             30035..30613
                     /locus_tag="KQD82_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG4380272.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12425 a         3055 c         5662 g         9519 t
ORIGIN      
        1 aaattgtgga attaaaatat actaggatat aaaattctta aataaaaaga aaagattgtt
       61 tttataaaaa atatttttta taccacttta aaaaacacaa taataacact gagggaggtg
      121 gggatatgag agaacgcatt attcttgaaa cattaaaaaa tagtagtggt tatctaaaca
      181 tagattattt tgcagataag ttgggggtat ctactcgaac tatcagaaat gaaataaaaa
      241 agatagaaac tataaaagaa tgtaatggtt ttaaattaga atataagtct aaattaggat
      301 atattctaaa tattaaagac caagataaat tcgagcatta tctaagaaac ttaccctcag
      361 atttagttga aaatccagaa caacggttgg agtctataat agtagagctt ttagtaaatg
      421 aaggttataa aactatagaa cagttaagta aaaaatttct agtaagttca tcccaaataa
      481 aaaatgattt aaaaaagata gatgaaaaga taaaggatac agaattaaaa ctagagcgaa
      541 aggctcatta tggaattaaa atagaaggtt ctataaaaga aattcaaagt atattggtgg
      601 atagttattt tagagggaat agaaatataa cagagtacag gaataagttt atagataatt
      661 taaaattagc taatattaga agtacaataa aaaatgtact taatgaacat gatttagaag
      721 caaatttaac agaacttgag gaaatattag cacaaattat tatattgtat ataagagtta
      781 gtatgagagt agtgggaaat gctagcgatt taaaattata tacagaagat ttaattatag
      841 ataaattatt agataaaatt tttagagata aaaaatataa ccttaattat gatgaaaagt
      901 attatttaaa ggagcttata aaactaaaga ctaaagataa gaaagccact ataaagaata
      961 ttgataagaa taaacttcaa gatattatgt tcgaattttt tagagagata gataaaaaat
     1021 ataactctaa ttttttagaa gataaagagt tttttaactt attttactta catattgcat
     1081 gtttaataga acgtataaag aagaaccaca agataataaa tcctttttca gttaaaataa
     1141 gtcaacagta tccaactgtt tttaacttat ctattcaatt ttcaaaaatc atagaaaatg
     1201 agtatcatat aaaaataagt caagatgaaa tagggtttat agctactcac attgctgttc
     1261 cttttgaaaa gagagaagaa gctaatttta ataaaaaata taaaatagca ataatatgct
     1321 cttctggtgg aggtagtgca tttttgataa atttaagact taaagaaatt tttccaaatg
     1381 ctgagataag aaatttttca ttactagaag aaaaagctgt aatagaattt gcaccagatt
     1441 tgattttttc aataacaaat cttttatttg aaataaatgc tccagttata cttataaatg
     1501 aaattttaga cgaattggat tatttagata ttaaagaaag tgttagattt gcagaccata
     1561 taggaaatat atcaagccca aaacaatata tattaggtct atttgataag aatcacttta
     1621 ggtgtataaa agataaagct aagtataagg atatccttga tagtatgtca aaatgtatag
     1681 ttgacgaggg agcttgttct ccaacctatc ccaaagatgt atgggaaaga gaaagctatt
     1741 tgagtactat ttatacaaat ggggtggcta tacctcatcc aatcgagatg acaggaaaca
     1801 aaaatataat atcagtagct ttgatacaaa ctgatattat ttatgaaaat agggtgccta
     1861 aaattatttt catgatttca ttaataaagg gaaatttaga acttcataag caaatatcta
     1921 aatatttaag taagattatg gcaaataagg atatggtaga catgctaaat aagtcacaat
     1981 cttatgaaga gtttatgtat aagttaaaaa tctatctggg aggttagaca tgaatataga
     2041 gttttttaag aggttgtcag aagcagatgg tatagcatct aatgaagaag aggttagaca
     2101 tgttttattg gaagaactaa aagcttatag tgataaaatt atctgtgatg ggctagggag
     2161 tatcatcttt tctaaaataa aagatgagag tgcaccaaac gtaatgattt gtgctcatat
     2221 ggatgaagtt ggatttatgg tgagaagtat agacaaactt ggtatgattc acttaataac
     2281 aataggagga gtaaaacctc ttgcacagtt tgtacaaaaa gttagaatta caacaaaaga
     2341 agggaagaaa atccccgctg tcataaatgc tacatataat aatggtaagg ctgaaaatat
     2401 atatgcagat attggagcat atacagaaga agatgtatat aacctaggga ttaatgttgg
     2461 ggatatggta acatatacaa caagctttga ggaatttact ttaccagacc gattagtggg
     2521 aaaggctttt gatgatagga taggatgttt tgtgatggga gaagttttaa aagaacttag
     2581 gaaagaaaat ctaaattgta atattcactt tgcggctaca agtagtgaag aggttggaat
     2641 aaggggagca aagacatcaa cacagctaat aaatccagat atagtatttg taatagatgt
     2701 agcatgtgct aaaaatgagt ttgtgagaga ctatacaaat cagaaacaaa ttgataaagg
     2761 tgtaatgcta atgcatagag ataggactct agtccctagt agaaaaatga tagattatgt
     2821 aatggaaatt gctgataaaa ataatatacc acttcaacat gatatgtttg aatcaggtgg
     2881 gactgatgga ggagaagcac atctcgtaaa tgaagggaaa ccttgtgtag taacttgtgt
     2941 accagttaga tatggtcatt gtgcattttc aatagtaagt aataaagatt tggagaacat
     3001 tataagattg tatacacagt taattttaaa ttttgatgaa gaacaatata aacatataag
     3061 aaattttatc taaaggagaa taagaaaatg tcaacagaag tacaaataat ggaacttata
     3121 tcaactgcag gagagagtaa atcgaaagct tttgaggctt taaaaaaagt gaagtcaaaa
     3181 gattttaaag gagcaaaatt gttaatagag gaagctcgta aaatagatat tgaagctcac
     3241 aggatacaaa cagaattaat aactcaagat ttaaatgaag atgttgaaag tccagcaatg
     3301 agtcttttaa tggtacatgc tcaagaccac tatatgactt ctcagctagc aaaagacttg
     3361 atagaggctt tgatatattg tctcgataag taattgtaat gtagaaatag attatttatg
     3421 taaaaattct tttaaaagtt tagatgcttt taagagataa taaaaataca aactttagga
     3481 ggaattatta tgaaaatatt attatgttgt gcaggtggat tttctactaa tatgttaatg
     3541 caaaatatga aaaaggtggt aaaagagagt gctaaactca atgaaaaaga ttttgagttt
     3601 acagctatac cagcagattc aatagaaagt gaagttgatg attgggatat agtacttata
     3661 ggtcctcaaa tagctcacaa aatagacttc ttaaaaggaa tattagagcc aaaaaataag
     3721 ccttttgcag taatagataa agatgtatat ggaaaaatgg atggagctac agttatgaag
     3781 ttggcattaa taacttataa gaaacatcaa ttagctcaag gataataaaa ataatgaggg
     3841 aaaagggaga ttagatatgt tttcaaaatt tgaagcattt atgaatagag tatttatgcc
     3901 tttagctcat aaggttgaca atcaacgtca ccttggttct ataaaggctg gtatggttgc
     3961 tatgacacca ttcacaatat taggaagtat atttgcaata cttccagcac ttccaaatat
     4021 gcttggagaa aataatccaa taagcaaatt catacttaac aattcagcaa tattggattt
     4081 accagtaagt ctttccatag gtttaatagg attatatgct tgtatgtgta ttgcttttaa
     4141 tctggggaac tactataaat tatatatacc aggttgtgtt gcactatcaa ctttttcctt
     4201 tttattccta gtagcctcat ttacaccaga tggagcttta gaaataggaa atttaggagc
     4261 taaagggcta tttacaggta tgatagcagg gcttggttct gtggagttat ataatttttg
     4321 taataagaag aatatcacaa taaaaatgcc agaaaatgta cctgattttg tatctaggtc
     4381 ttttgaatta atacctacta ctataatagt ttgtggaact tttatagttg ctcgttttat
     4441 ttcattaaaa gtatttggag aattacctcc acagatactt actcaatttt tatctccatt
     4501 agtaggaagt atggataacc catggtcagt tcttgcactt aactttgcta tatgtactat
     4561 attctttttt ggaatacatt catcagtatt tagtcctata actagaccta taatgattac
     4621 atttatagct gaaaacatag cagctatgca agctggagaa acaatacctc atttctttac
     4681 agcaggagct tcaagtgcat tctttggatt tacaggatgt ggaattagta taggttgtgt
     4741 aattgcatgt atgttatcaa aaaatgcttc ctataagaag attggacgtg tatcattatt
     4801 ccctgcatta tttgggataa atgaaccaat attatttgga gcaccgataa tattaaatcc
     4861 aataatgttt ataccacatg tatttggtgg agctataata ggtacactac caatgttttt
     4921 tatgcactgg ggatggttag caaagccagt atttgaccct ccatatgtag gggtattctt
     4981 agaaggattt ttaactaatg gagattggca ttctatatta gcaaatgcat tgcaattagt
     5041 attagcagtt ttattatatt ggccattctt taaaataatg gaacgtcaac aaaataataa
     5101 aaaacaaaaa gaagaaacta agaatatatt ctctaaagaa gaagaagact tattggatga
     5161 tttaggattg gatttctaat attttgttat gaataataca atggagagat tagtatcctt
     5221 aatctataaa agaggtgttg acgctctttt gattaaagca aaaaatacta aaagatatat
     5281 aggagcttta tcaggtagtg gtgtatatgt gcttgttact aaggataaaa attatcaaat
     5341 tctagatggc agatatattg atgaagcaga taaaaaaacg actggtttca taaaaaggat
     5401 tgtatctcaa ggttcttata taccaactat aattgagtta ttaaaagaat taaatataaa
     5461 aaagttagca atagaaaacc aagctatgag tatacaagag tatctttcac taaaaaatga
     5521 aggttttgaa atcaatttaa taacaaatga attatggaag ctaagggcta taaaatctaa
     5581 agaagaaatt gaacttatta aaaaggcttg tgaaattaca gatgaagtat ttgatgaggt
     5641 tatttctgaa attaaaaaag atatgactga gctagaagta tcagctctaa ttcagtatca
     5701 tgcattaaaa aaaggtgcaa gtggaatgtc ttttgaaaca atagtggtat caggagaaag
     5761 aggtgcaatg ccacatggaa gacctacaaa caaaaaattg aaagtgaatg aagctataac
     5821 aatagatttt ggtgttgttt atcaagggta tcaatcagac atgactcgta caatatctat
     5881 aggtaaacct cctaaaatta ttaaagagat atatgatgta gtattggaag cacaattatc
     5941 tgcaatagag tcaataaagg aaggaactag ggcaagtgat gttgacaaag tggctagaaa
     6001 aattatagat aaacatggat ttggcgaata ttttaatcat ggactgggtc atggtatagg
     6061 tcttggagat ggtgaagttc ccactttaaa tccaaatagt gaagatatat tagtggaagg
     6121 aatggtaatg tcttgtgagc caggtattta tattccaaat gtagggggag ttagaataga
     6181 agatgatatt gttataatag atggaaaagg tgttccactc aataagacat ccaaagagtt
     6241 tataatatta ggagagtaaa attatgaaat ataattttaa taaagtatta aatcgtaaag
     6301 aaggaaattg tagaaaatgg agcaaccatg tgattaaaga aaaatttggc ttaaatgaag
     6361 atgcaattcc tatggattta gctgatatcg attttgagtg tgctcctgct ataaaagaat
     6421 ctatcttaaa aagagcatca gtaggagatt atagttatac ttttatagga gatgacttct
     6481 atgatgctat tataaattgg aataaaagaa gatttgatgt atatatagaa aaggattgga
     6541 taaaattaac ttttggtact gtttcaaccc ttcattatat agtccaagca tatactaaag
     6601 aaggagaggg tgtattgata aatacacctg cttatgaccc ttttgcagag gctgtagtaa
     6661 ataataatag aaagttgtgt tgtagctcat taaaattaga taacaataga tattatttag
     6721 atttagagga tatggaaaat caaatgaaat acgagaatat aagattgctt atattctgta
     6781 gtccacaaaa tccatcaggt cgtatatgga caaaagaaga actatatcaa gtgtcagagt
     6841 tatgtttaaa gtataatgta attttggtaa gtgatgaaat tcatagagat ataatattta
     6901 aagattatag atttgtatct ttgtggaatg ctcatccaga aatttataaa aatagtataa
     6961 tctgtgtatc tcctaataaa ggcttcaact taggtggatt aaaaacatca tatgtattaa
     7021 taagagatgt aaaaattagg gagactttat tagaaaggct aaaaagtaat tcaataactt
     7081 ctccaaatgt atttgcaata ccagcaatag ttgcagcata taatgaaagc gaagaatggc
     7141 tagatgctat gactagttat gtagaggaga attttgaaat agtttatgat ttctttgaaa
     7201 ctaatatacc aaaagctaaa gtgatgaagt cagactcttc ttttttagcc tggatagatg
     7261 taagagaagt atttagaaat gaagaagaat ctaaagaatt ttttagacat gcaaacttaa
     7321 ctatggtagt tggaagttat tttgtgaaag atggagatgg atttattcgt attaatgtgg
     7381 ggtgtcctag agaaacatta aatgaggctc taaatagaat caaaaaaacg tatataagta
     7441 tgtattgtta aatcataaaa aaagtatcta tggtttatat aaaaacacat tatttagtta
     7501 tcaaatatta ctggaatttt gtattccagt aatatttttt gattaaggaa tattattgag
     7561 gaatattaaa taatattatt tcataccctt atattaactt atataaaaat attttattat
     7621 tttgggaacg ggttagctaa cctttatcta gtgtatattt atattgtaag aagcagtaaa
     7681 aacttttcct ttactaagtt tattaataaa cttcttatct ttataaattt tgttagaatt
     7741 tatactatca gcaagaccat accaaggttc tatacataaa aaaggagcag tagaattagt
     7801 ttcactataa taaggagtcc aaataccaac tagagggaaa tcttccatag atatagttac
     7861 atatttacta ccatcattat tacatataga cacttcatct atgttggtgt atatcaaagc
     7921 atcattcttg aatacttctg gatttaactg taagtttttt aattttccaa taggtattat
     7981 atcatctgta aaagaaccat ttaaattgat tttttctaca tcatttttac ttttaaactc
     8041 aaggtagtgt ttagaaaaat cattttgttc gtaaaaagga aggttgaatg caggatgtcc
     8101 acctatagaa aagaatatat ctttagaatc agtatttttt acaatccatt ctacctcaat
     8161 actattatca tttaatgtat agtttatgat tagctcaaaa gaatatggat acttctttaa
     8221 agtagattca ttatctgtta atttataagt tacaaatgta tctcctttat ctacaatctc
     8281 aaagtccata tctctagcaa acccatgttg agtcatgcta tataaatttt cttctattat
     8341 tgtttcatta tcaaaaagtt tacctactat agggaacaat ataggtgatt gtctacccca
     8401 atatttttta ttaccatccc aaagaacgtt tttgtcgtat tttttagaaa atattttagt
     8461 tagttcagca ccaaaactgc ttgattcgat aattaaattt tcattttgta atatattcat
     8521 ttaaaatctc ctttattata ttttatgagt atattatact ataaagtata aagattatta
     8581 aattttattt gatattatat ttgaaaggta gattttagta tattttgaaa attttgtttt
     8641 gaaaatttgg gattatttaa gaaattttgt attataaata gaaggaaata gataaattgt
     8701 atagaataaa atatattaag ataagttaat aatgttaaat gggaagtgag tttatggaaa
     8761 aaccaaatac aaaagttgtt aactttcaag agtataaaga cagaaaaaac caaagagaag
     8821 taaatagaga cgaactttta aagcttataa aaaaaattgt gacttcaaaa tagggagttg
     8881 ttaaactccc tattttaatg ggtttcataa taattaagac attctaagtc catctatata
     8941 atctttgaat tgtgagaaat ctgattcact acttaattct atggtttctg aaccttccgt
     9001 ataaaaattc ttaagataga tagtataatt attatcaaca caatcatgat aaatacatct
     9061 atatccatct tcatacaaac tttttaattt agaaaaatca tccacaatat catctcctta
     9121 aagttctttt aaatatattt ttacaaaaag catctaattg tatgcaaatt gacaatgtaa
     9181 aataaaggaa atgaacataa aatgtagaat aatttttata tgtatgtaaa aataaaaaga
     9241 aaaagttgaa atttttagaa aataagagta ttttttatct aaaaagagag tttaaaaaat
     9301 aaaaaaatgt tgggaggata aaaaatgagt ttattagaaa gtatatcaaa aaacatatat
     9361 tccctagata actcttctat tgaaaaaaca aagcagagat tggataggct tatacatcca
     9421 actggaagct taggaaagat agaagatatt tgcatgcaat tagcaggaat atttggcaat
     9481 gaaaattttg acacaagtaa aaaggttata attgcttttg cgggagacca tggtgtatat
     9541 gaagaaggcg tagcacctga ccctcaaaat ataacaaaat tacagtttcc aaatttttcg
     9601 aaaggtctat gtggagttgg agtaataagt aaatttgtag gggcagatgt tgtagctgta
     9661 gatgtaggaa taaactgtga tgaaaaactg gatggggtat tagactacaa gataagaaaa
     9721 gggacatcaa atatggcaaa aggtccagct atgagtaaac aagaagcaat tagatgttta
     9781 gagataggta ttgaaatagc agagcagtgt atagaaaaag attataaggt tattggtatt
     9841 ggggagatgg gtatagcaaa tactactcca agtacagcaa taatatctgt aatttcaggg
     9901 tgtgacccgt tagaagttac tggtataggt gcaggtctta agaaagaaag actaaagcat
     9961 aaggctgaag taattagaaa ggctattgaa attaataatc caaatccaac tgatggagta
    10021 gatattttat ctaaagtagg aggatttgaa ataggctcaa tggcaggtgt tatattaggt
    10081 tgtagtgcta ataggattcc agtagtaatt gatgggttta tttcttatgc agcagcatta
    10141 attgcatata agataaatcc aaaaactaga gaatatatga tagcatctca tttatcagca
    10201 gaatcaggaa ctaaaagagc attagacata ttaaaattag accctctatt aaatatggat
    10261 atgagattag gtgagggaag tggagctgct ttagcatttg atataataga agcttcaaac
    10321 tatacttata aaaatatggc aacttttgat gaaattgaca tgggaagata aatataaaaa
    10381 tttaatatgt aggagtgttt tgatgagtaa aattattcta gtaacaggag gagctaggtc
    10441 agggaagagt aactttgcag aaaaactatg ttttgacaga aataataata cagcttatat
    10501 agcaacctct attccatttg atgatgaaat gaaagataga gtaaaaaaac atcaagagaa
    10561 tagacctaga aactggaata cttatgaaat atatgaagat atatattcca taattaagga
    10621 gatatataaa gaacatgaaa ctgttatact agattgtgta acattattag taaataatct
    10681 aatgtttgaa cacaatttaa atatagaaga atctagtcaa gaagaaataa acaaattaga
    10741 acaatacata aaagaacaag ttgtcaaact tattgaagaa atagaaaaaa caaatctata
    10801 ctttgtattt gtaacgaatg agttaggtat gggaattgtt cctgggaata aacttagtag
    10861 aatttatgct gatattgtag gtcgtataaa tcaatatata gcttcgaaaa gtaatgaagt
    10921 ttattttgta gttagtggca tagctatgaa gataaaggag tagtatgcaa ttatgaaaag
    10981 atttatttta atactacagt ttttgacaag gatacctata aaattaaatg taggatttga
    11041 tgatgaattt tataagtcaa tagtatattt tccacttgta ggttttgtca taggtatatt
    11101 atcctattta ataggatgga tttctatgct tttatttgaa ccatttatag cttctataat
    11161 aataactcta gctggtgtct taattactgg aggacttcat atagatggat taggagatac
    11221 atttgatgca atatatagtt atagagataa agaaaaaatg cttgaaataa tgaaagattc
    11281 aagattaggt acaaattcac ttctagcaat tatgtttgta cttttattaa aggttggatt
    11341 cgtttacgat ataataagta ataattcatt gtgggttata atttttatgc caatgattgc
    11401 aaggttagga gtaatgttat tgacatataa aactgtgaca ccaagagaaa aaggaatggg
    11461 aaatttattt ataggaaaat taactacaag tatgttaata actgctataa tatacacatt
    11521 attgatagtt gctcttatta ctaaatttat atttttatta cctaatatag tgttgattaa
    11581 agtattaggt tcgattatag tggtttttgt atttataata ttattcaaaa aacatattta
    11641 taaaaaaatt gatggagtaa caggagatat attaggctgt ggaatagaac tttcagagct
    11701 tgtatattta atttacatat atttgttaat ttttatgttt ttttaaagta aaaagtggtg
    11761 ataatatgat aagattaata ttaataagac atgcactgac aaatgataat aaaaaaggaa
    11821 gattatcagg ccatataaat agttgtatta gtgaagaggg caaactacaa ataaataaaa
    11881 ttaccagata tctgagtaat gaaaacatag ataaggttta tacaacacca tctacacgta
    11941 ctaaagatac agtagaaaaa atatcaaaat tgaaattatt agaaatagaa gaaaaagaag
    12001 ctttaagaga aattagtttt ggagactttg aaggaagaac ttttgaagaa attaaaatca
    12061 aatatccaaa tgagtttgaa aaaatgataa aagaaggtaa caactataga tatccaaatg
    12121 gagaaagttt gatagactct tataaaagag tggcaaaaga aattgataat ataattttag
    12181 aaaatgatag taatttagat acaaagacaa tacttatatg ttctcatgca ggaacaataa
    12241 gaaatataat aacacatttg atttctggta gttataagta tcactggaac tttaaaattg
    12301 ataatgcatc tattacagtt ttagaaattg atggtggatt tgctgtaata gataaaatga
    12361 attttacaga cttcatataa tttaatcaaa accaaacatt acaatctgtg tttgtaatat
    12421 atctgtaaaa ttagcaatga aaatagaata ttagattaaa attaggttct taattagatg
    12481 ttaaaaggga aaaaggttag atgcctttgc agcccccgct actgtgaaac caacgaaact
    12541 ataaatacca ctgtcagttt gatgggaagg ttatagaagt aggatgaagt taagccagga
    12601 tacctgccta atttaattta aaacaagagg tcttcggggt gaaagatttt taattaatat
    12661 atataaaagt cctatcttta gaagtattag gacttttttt aatttgtttt ttagtaaatt
    12721 ttaacaagga gggatggaaa gtggggaaaa aaataatgct acaaggaaca gcttcaaatg
    12781 taggtaaaag tgtcttagta gcaggtttgt gtagaatatt taagcaagat ggttatactg
    12841 ttgctccttt taagtcacaa aatatggctt taaattcttt tataactaaa gaaggattag
    12901 aaatgggaag agcacaagta tttcaagcag aggctgcgaa aatagagcca atagctgata
    12961 tgaatccagt tttattaaaa ccttctggaa ataataaaag tcaagttatt gtaagaggta
    13021 aagttgtagg agaaatgcct tcttctaagt atcatgatta taaattggag ttagtagatg
    13081 tgttgaagga aacatttaat agcttaaatt caaggtatga tattgtagtt atggaagggg
    13141 caggaagtaa tgcagagata aacttaaaag atagagatat ctctaatatg ggtatggcag
    13201 aaatagctga tgcacctgta ataatagtcg gagatattga tagaggaggc gtatttgcct
    13261 cattagcagg aacaatgctt ttattaaatg aagatgagaa aagaagagta aaaggtgtaa
    13321 taatcaataa attcagagga aaaaaagagc ttttacaaga tggtgtaaag atgctagaag
    13381 acatcataaa agtacctgtt ataggagtag taccttatac agatataaaa atagaagatg
    13441 aagacagtgt aactacgaga tttaagaaaa aaatgaataa aagtgatata cacatagaaa
    13501 taattagaac cccacatatg tcaaatttta cagattttaa tatatttgag acacaagaag
    13561 atgtaagtat tagatatgta gactatggag aatctttagg aaatccagat atattaataa
    13621 tacctggaac taaaagtact atagacgatt taacatatat aagaaaaagt agtcttgaat
    13681 cgcaaatcaa aaatttacat agtcaaggta aattgatatt tgggatatgt ggtggatacc
    13741 aaatgttagg taaaaaatta aaagaccctt atcatgttga aagtgaaatt gaagaagttg
    13801 atggaatagg tcttttagat acagaaacaa cgtttgagaa agaaaagact acaacacaag
    13861 tagaagctac tattgttgaa tgtatcagtg aatacatgaa taaccttaag ggaaaaaaag
    13921 taaaaggtta tgaaattcat atgggaatta caaagcgagg taatgatgta agaagtttag
    13981 atttaataac taaaaaactt gataaagaag tgaattatac tgagggaagt gtaaataaag
    14041 aggggaatgt aattgggact tatcttcatg gaatatttga tgaaataagc tttacaagag
    14101 caattttaaa caatataaga aagaaaaaag gtttaggaga attagaaagc aaagtaaact
    14161 cttttaatga atttaaggat aaagagtatg ataagttggc tgatttttta agagaacatt
    14221 tagatataga gaaaatatat gagattatga aataatgatg acctttattt ataatttaag
    14281 taggaggtgt attatgaaaa aaattttaat tgcgggtact aatagtggag taggtaaaac
    14341 aacaatatca ttaggaataa tgcaagcttt ggttaagaga aatatgaaag tacagccata
    14401 taaagtaggg ccagactata tagacccttc atatcacact tttataactg gaagacattc
    14461 tagaaattta gattcttata tgttagatga tgaaaaaata aagtatatag taaataagtc
    14521 ctcaaaagat gcagatattt cagtaataga aggtgttatg ggcttatatg atggctttgg
    14581 aatagattta gataattgta caagttcaca tacttcaaag gtattaaagt cacctgttat
    14641 attagttata aatggtaaat ctatggcagc atctagtgct gctatggtac ttggatataa
    14701 ggaactagat aaagatgtaa atataaaggg tgttatagta aataatgtaa agactaatag
    14761 ccattatcaa ataattaaga gttctataga aaaatattgt aatgttgaag tattggggta
    14821 ttttcctcca aataacaaat tctctttgga atctcgacat ctaggattag ttcctagtgt
    14881 agaaatgaaa tcactaaggg aaaaatttta taccttagca gatgaaattg aacactatat
    14941 agatattgac agaataattg aaatatcaga aagcgatgaa tttgagagtg attttgattt
    15001 tcaaacttta atagaaaata ataaaataaa agaacatgtt aataataaat caatagctat
    15061 agcatatgat aaagcattca atttttatta tagagaaaat atagagcttt tagaggaact
    15121 tggtttagaa ataaactatt ttagccctct tgaagatgta tcagtgccaa atgcagatta
    15181 tatttatatt ggaggaggtt ttccagaaat atttggaaaa gaattagaag caaatgagtc
    15241 aatgagaact tccatattag aagcacataa caataatata cctatttatg cagaatgtgg
    15301 aggactgatg tacctaggtg agaaactatt gaatcaagaa gaacaagaat ttaatatggt
    15361 tggaatattt agtggaatta gtaagatgac atcttcttta aagagatttg ggtactgtat
    15421 tggtgtagcc aaggaaaata ctcttttatc taaaaaaggg gaatctatta aagggcatga
    15481 gtttcatcat tcaatatttg aaagtgatga aaaatgtgta tattcaatga gaaaagaaag
    15541 agatggaaat atagtagaag agtgggatgg tggttatagt aaaggaaaca ctttggctac
    15601 atatcttcat actcattttt ataataactt aaattgtata gaaaattttt tgaaaaaagg
    15661 aaatgaataa atgagtattt tatctattta tataggatat gtgacagact taattgtagg
    15721 agacccatat tcttttcctc atccagtgag atttattggt aaacttataa attttactca
    15781 aggtataata agaaaggtct ctaaaaatga taggcagtta aaatttgggg gatttatatt
    15841 atggtttata accgttggaa taacatattt aacaacttat gttatagtga agttatttag
    15901 ttttaatata ttattgagta ttgtagtgaa tagttttata atttatacaa cattagccac
    15961 aaaatgttta aaagacgaag cattaaaaat ttataatgta ttaaaaactg aagatataga
    16021 aaaatctagg attcagctat cttatatagt aggtagggat actactaatt taagtgaacc
    16081 tgaaataata agagcaactg tagaaactgt tgcagaaaat actgtagatg gtataattgc
    16141 gcctatgttt tatgcattta ttggaggagc accacttgct atggcctata aagctatcaa
    16201 tacattggac tcaacagttg gatataaaaa taaaaagtat aaagatattg gttttgcatc
    16261 tgctaagatt gatgatatag ccaattatat accagctaga atatctgtca ttcttatgac
    16321 aattggaagt ttctttttaa attataacta taggaactgt tttaaaatct ctattagaga
    16381 tagaaagaat cataaaagtc caaattgtgc tttttcagaa ggtgcagtat ctggtgctct
    16441 aggaatacaa ctaggaggta ccaacgttta ttttggggaa aaagtatata agccaacaat
    16501 aggggataag cttagggaga tagataaaga agatataatt aaaactaata aaattatgta
    16561 tgcatcttca tttgtatcta tgttggtatt tacactaata tatgtgttat gtaggatgat
    16621 tttaattaac ttaattatat aaattattgt ttgttaactt aagggggaca tagatgaagg
    16681 atttagggca tggtgccaat gtagatgaaa tggcaagatt atatgggaaa aacccaaaag
    16741 aaataattga ttttagttca aacataaatc caaatgtatt accaaattta gagagataca
    16801 ttttaaaagg attagaagag tgtaggaatt atccagatat aaattataca aacttgagag
    16861 aaaatatatc aaaatatata gatataaatc cagactttat aatacctggc aatggtgcaa
    16921 cagaagtgat ttatttgctt atgaagagta ttaaaaaaaa attggctata ataaatccga
    16981 ctttttcaga gtatagaaga agtgctgagt taaataattt agatattata gatttagagt
    17041 tagatttaga aaataacttt aaacttaata tagatataat aaaagaaaat ataaaaagat
    17101 ttgatagttt atttatatgt aatcctaata atccgagtgg taatgtccaa gatttaaagg
    17161 aacttgttca tttactagat aaacataata aggtactaat aattgatgaa actttcatgg
    17221 aatttgttga agatgaaagt aaatatagtt tggttaaata tatagaatca aataaaaata
    17281 tatttataat taaagctgtg actaagtttt ttggtatgcc tggtttaagg ctaggatatg
    17341 gtcttacaag caacactgaa attatgaaca aaatttatga acacaaagag ccatggacta
    17401 tcaattcttt tgcagatata ctatccaatt ttatttttga agataaagag tatattaaaa
    17461 atagtaaaga atattacata gaagaaagaa aatatatgtt acaagagttg agaaacataa
    17521 gaaatataaa ggtctatgat acagatgcta attttatctt gataagaata tataaaaaaa
    17581 caactaaaga attgaaaaaa gatttgttta agcaaggaaa catattagtt agagatgcat
    17641 ctaattttat aggattagat gatagtttta taagagtggc tataaaatct catgaagata
    17701 acaaaattct tatagaaaat atgaaaaatt tattgggtga ttaaaatgaa atcttatgga
    17761 atatgcccag catcttgtgg agaatttgta caaggtataa ttgatgatga agaatattta
    17821 tgttcatatg caatagatat gtattctaaa gtgtatattg aagaaaaatt agtagatata
    17881 aatttaggga gatataagtc aaggttagca attgaaaagg tatttgaaaa atttaattta
    17941 cctaaaaaat atactaaaaa tatttcttta aatataaata gtaaaatacc tgttggaaaa
    18001 ggaatggcaa gttcaactgc tgatatagga gctactataa aggcaacatt atcattgata
    18061 gataaagatt taagtagcga ggagatttct aaattagcag cagaaataga accaacagat
    18121 tctatattta tagataaaaa tagtatattt aatcctctaa atggaactgt tataaaatac
    18181 ctaggaaatc tgaccaatgc gaaagttgta atacttgaac caaacaaagt actagatact
    18241 atgaagatta gattgaggca agattataat aaattaaaag tagagaataa agaagtaata
    18301 aaaaaatcat ttgctctttt agaagaagga ttgaaaaaaa ataatttatc tctagttgga
    18361 gaggcttgta ctttgagtag cttagcaaat gagaatatag aaaaaaaaga gtgtttaaat
    18421 gaaataataa aaatatcaaa aaaatatgga gcatatggag taaatattgc tcacagtgga
    18481 actgtagtag gtattttaat tgataagtct atgaatgata aaaagatgat agatgcctta
    18541 tgtgaatcta acataaattc tgtctataat aaaatttata cacaaaacat aattaatgga
    18601 ggaattaagg gggaaataga atggaataca taaagaatcc tatgaaaata gaagaaaaaa
    18661 gttttgaatt gatacaagaa ataatagacg aaattagacc agattataaa tttaagaata
    18721 atatcgaaga aaaaataata aaaagagcta tacatactac tgctgatttt gattacttag
    18781 atatattaaa aatatcagaa gaagcagttg atagtataat agatgcatta aaaaataatg
    18841 caagtattta tactgataca aatatggctc ttagtggtat aaataaaaga aagttagaat
    18901 cattaggatg taagtataaa tgtttagtgg cagatgatga aactgtaaaa ttagctaaag
    18961 aaaaaggaat tactcgttct atggcagcag tagaaattgc agctaaagaa gaaggaagaa
    19021 aaatattcgt tttaggtaat gctccaacag ctttatataa agttatggag atgaaatcag
    19081 aaggtagatt agatttagat gcagttatag gagttcctgt aggttttgta ggagcacaag
    19141 aatcaaaaga tgaagttgaa aaaacagata taccatatat aatttctaaa ggtagaaaag
    19201 gtggcagtaa tttagcagca gcaatagtaa atgctatatt atatactatg taggtaccta
    19261 tatggaagaa tatgtatata tagatggaaa aaaatataga agaggatata ctacaggttc
    19321 atgtgcaaca ggtgcatcta aggctgctgt atatatgttg attacaaaaa atagaatcaa
    19381 tactataaat atagatactc ctaaggggat accattacta ctaaaagttg ataatataaa
    19441 tatttctgat acatttgtgg aatgttccat aaaaaaagat ggtggagatg atatagatgc
    19501 aactcatacc atggatattt atgcaagagc tgaaattgta gctaaaaatg ataagaacaa
    19561 agggtatctt actctaaagg atatagatag tttaagcacc aatagtgagt gtaaaagtga
    19621 gctctataag tttattaggg tttatggcgg aactggtatt ggtgtggtta cgaaaaaagg
    19681 gcttagtgtg gatgttggta agcctgctat aaatccgact ccacttaaaa tgataaatca
    19741 tgaaataaga aagcttatag gagataattt tgaatctata cttggaaatg ataaagtttt
    19801 aaaaataaca atctttgctc cacaaggaga aactgtagca aaaaaaacat ttaatccaag
    19861 attaggtata gttggaggaa tatctataat aggtacaact gggattgttg aacctatgag
    19921 tgatgagggt tggaagaagt ctctatctat agaacttcag atgaaaaaag aacaaggctt
    19981 agataaaata atattggttc caggaaatca tggagaacag tttataagag aaaaattaaa
    20041 cttagatatt aagtatgtag ttagggtaag taattttata ggctatatga ttaaagaagc
    20101 tcaaagaata ggatataaga aaatcttaat ggcaggtcat ataggtaaat ttataaaagt
    20161 atctgctggt atttttaata ctcatagtaa ggtagcagat gctagaagtg agatattagt
    20221 ggcaaactta gctttaatgg gagctagata cgaattttta aataaaataa atcaatgcgt
    20281 aactactgaa gaagctgtag agctaataaa taatagtgaa tatagagaag tatataatat
    20341 attaagtaat aaatgtaggg agagagtaaa gcaatacttg aatgaagatt cagatgatat
    20401 tgatgtggaa gtaatcatat tttcaatgga taagtcttta ttaggtaaat cggataatac
    20461 agatgattta gtggaggtct ttatatgata aacataatag gtctaggacc tggcaactta
    20521 gactatataa caaaaaaagg tgaaaattta atttccactt ctgatgtatt aattggtggt
    20581 aaaagaaatt tagagtctat aaaaaatttt gaaggagaaa aaatagttct ggattctaat
    20641 ttaagagaaa ttatagagta cataaataac aataaagaaa agcaaatatc aataatagca
    20701 tctggggacc cattaatata tgggataggt agatatctat ctaaaaatat tgataataag
    20761 atgttaaaca tggtttctgg gattagttca ttacagtaca tattttcaaa aatatatgta
    20821 gatatgaatg atgtatatat tacaagtagt catggcaaag taccagattt tgattacata
    20881 ttatcacata aaaaagtatg tatggttaca gattcaaaga taggaccaaa acaaataagt
    20941 agagaaataa tagatagaaa tctaaataaa attattgttg taggagaaaa tctgtcatat
    21001 gacaatgaaa aaataacaat agcaaaacca gaagagataa taagaataga caattttgat
    21061 atgaatgttg tggtgatttt agatgaggaa tagtgagttt ataacaggta aagttccaat
    21121 tactaaggaa gaggttagag caatatcaat aagtaagtta aatttagtaa atgctaaaaa
    21181 cctcatagac ataggggctg gtacaggaag tgtaagtgtt gaagctgcat ataattatcc
    21241 taatttaaag gttatatcta tagagaaaaa tgatgatgct atagagctta tagagaaaaa
    21301 taaaaaaaaa ttcaacttaa aaaatataga agtgataaaa ggatatgctc caattaatct
    21361 agggttaaat gttaaagttg agtctatttt tcttggggga acaggaaata atctggaaga
    21421 aataatagaa tggtctaaaa aaaatctcgt tacaggagga agacttgtag ctaattttat
    21481 aataatagat acatttaatc aaactttaaa actgcttaaa aagcatggat ttaaagaaat
    21541 agatgtatgt gttttgaatg tttctaaact agagaaacta ggaaaaggtg aatattttaa
    21601 accactaaat cctatatata taatatcttg tgaaaaaggg gaaaatgatg atgaataaag
    21661 tacattttgt aggagcagga cctggagata aagagctgat aacattaaaa ggatacaaat
    21721 tattaagtaa tgcagatgta gtgatatatg caggttcact tgttaatcct gaacttttgg
    21781 aatactgtaa agaagattgc caaatacata atagtgcaca tatggattta caagagataa
    21841 tagatgttat gagagaaggt atagaaaata ataaatctgt tgtaagactg caaacaggtg
    21901 atttttctat atatggttca attagagaac aagtggaaga tttaaataaa ttgaatatag
    21961 attatgactg tactccagga gtaagttctt ttttaggagc agcatcatct ttaggggttg
    22021 aatatacagt tccagaaatt tctcaaagtg taattataac aagaatggaa ggcagaactc
    22081 cggttccaga gaaagaatca atacaatcat atgctaagca tcagacatct atggtaatat
    22141 ttttatctgt tcaagaaatt gagaaagttg tgtctaaatt actggaaggt ggatatccaa
    22201 aagatactcc tatagcagtt atatacaaag caacttgggc agatgaaaaa atagttaaag
    22261 gaactttaag tgatatagct gtaaaagtta aagaaaacaa cataaataag actgctttaa
    22321 taatggttgg gagattttta ggagaagagt ataataattc aaagttatat gataaggatt
    22381 tcaaacatga atatagaggg taatattgct ataatctgta ttacagaaaa tggaaaaaaa
    22441 ttagctctaa agataaattc tctagtaaat aactgtgtta tatatcaagt aaaaaataaa
    22501 aaaagtaatt tagttgttga agaaacatgt gtacatacag ttcaaaagaa gttaagtgat
    22561 tttgtaggag aaatatttag caaatttgat tacatagtat ttataatggc tacaggtata
    22621 gttgtacgaa gtatagcacc atacataata agtaagttta gtgaccctgc aattttggta
    22681 atggatgaaa aaggcaaaaa cgtaataagt ttattgagtg gtcatattgg aggggcaaac
    22741 agcttgacac agtacataag tagtcttatt gatgctaatc cagtaataac aacagctact
    22801 gatataaatc aaaaagcttc cttagatatg atagcaaaaa aactaaatgc acatattgat
    22861 aattttagag aaagtgtaaa agaagtaaat tctatgctag tgaataataa atgtgttgga
    22921 ttatatattg atgaaggata tcaagttgat actaggggat ttaaacttat agaaagtaag
    22981 gatgagttaa gagatagttt ttcaaacaca tatatagaaa aattagttgt agtaagcaat
    23041 aaaataaatt gtattgaaga tattgagaat ttaaacctag ataataaatt tgaacattgc
    23101 tatagtacca aaaaaataat aaaggtaatt ccaagagata tagtgatagg aattggctgt
    23161 agaagaaaca cagatagtga gctgatgtat aattcacttg aagagtttct taataaacaa
    23221 aatatagata ttagagcaat aaacacagtt ggaagtatag aactgaaaaa agacgaaaaa
    23281 gcaataattg aattatcaaa aatattgaag gtgccattta aaacattttc agtagatgaa
    23341 atatctaagg ttgataatct ttttgataaa tctgattttg ttaaaaaaaa tgtgggcgtg
    23401 tattcagtag cagaacctgt agcatatctt ttgagcaaag gtaatttgtt agttgaaaaa
    23461 cataaatata aaggaataac tttttcagtg gggagaataa aaatatgata tacgttgtag
    23521 gaattggacc aggaagtaaa gatacaatga ctttagaagc aataaaagca atagaagatt
    23581 ctgaggctat tgttggatat aagacttata taaaactaat agaagaattt atacaagata
    23641 aagaaataat ccaaaatggt atgaggcaag aagttgatag atgtaaacaa gcagtagagg
    23701 aagcaaaaaa aggaaaaaaa gtagcagttg taagtagtgg tgatgctgga atatatggga
    23761 tggcaggtct tattttagaa ttaatatcta aagaagatga agatataaaa gtaaaggtaa
    23821 ttccaggtgt tactgcaagt attggtgcag cagctatact tggagctcct attatgcatg
    23881 atttctgtca tataagtttg agtgatttaa tgactccgtg ggaagtaatt gaaaaaagat
    23941 tgagattggc tgctgaagcc gattttgtaa tttgtttgta taatccaaga agtaagggaa
    24001 gaagtgaaca tctaagcaaa gcatttaaaa taatgggtga gtttaaaagt ggagatacac
    24061 ctgttggagt ggtaaaagat gtaggtagag aaaaagaaga aaagcttgta tgtacttttg
    24121 aaaatatgga ctttgaaaaa gtcgatatga ctacaatggt tataatagga aataagtcta
    24181 cctatattaa tggtgaattg atgataactc caagaggtta tacagtatga ttttaatttt
    24241 aggaggaact tcagatagtt tatctatatg tgataagata aatgaactga aaaatcaacc
    24301 atatatcctt tcagttacta cagattatgg aagggaactt gcacaacaac atgcagagaa
    24361 tgtaatttta ggtaagcttg gaaaagaaga tatgctaaaa tttataaaag ataataatat
    24421 agtaaaaata atagatgcca ctcatcctta tgcagttgaa gtatccaaaa ctgctattac
    24481 ttgtgcaaaa ctattgaata tagattatat aaggtttgag agaaaatctt taattgaaga
    24541 aatagattat gataataaat ttatagtaga tacaattgaa gaagcctgtg aaatagcaaa
    24601 taaaataggg acaaatatat tcataggaac aggaagtaaa aatttgaaca gatttataga
    24661 ggaaataagt gataaaaatt tagttgttag agttcttcca acgagtgaag tcatattaag
    24721 ttgtgaaaaa ttaggtttaa gtgcagataa tatagtagct atgaaaggtc catttagcca
    24781 gtatataaat gaagaaacat ataaacatta caatatagat ttagtcataa caaaagaaag
    24841 tggaatagct ggtgggttct tagagaaggt aaatgcatgt aaaactttca atataccagt
    24901 aataattata aaaagagaat cgattaacta cgaaaatgta tttaatgaca taaataaaat
    24961 taaagacata cttgtgtaat ttttaggagg agaatatatg aagaaagcct tattagtagt
    25021 gagttttggt acaagctatc atgaaacgag agaaaagaca atagatatat gtgaagataa
    25081 aataaaaaat tcgttaaaaa gccatgattt ttttagagct tatacatcaa atatgataat
    25141 aaataaaata aaaaaaaggg atggaataga aatagataat ccaatacagg ctttagataa
    25201 aatatatgaa caaggatatg atgaagtaat aatccaaaca cttcatatta tatgtggcga
    25261 agaatttaac aaattaaaag aacaagtaga tggatattct tctaaattca aaaagattgt
    25321 attaggaaga cctttgctta cacatattga agattatcag gaagctgtag aggcaattaa
    25381 gcatcaaatt cctcatatgg aaagtaatga agcagtagta tttatgggac atggaacact
    25441 tcatgaatca cattcagcgt atccagcatt ggaatacatg cttagggata atggaataaa
    25501 tgcttatgtt ggtacagttg aagggtatcc agaaatagaa catgtaataa gaagattaaa
    25561 agaaggaaat ataaaaactg taaatttgat gccatttatg ttagttgcag gagaccatgc
    25621 tataaatgat atggcaggag atgaagaaga ttcttggaaa acaatattag aagaaaatgg
    25681 atttaacgta agagttcatt taaaaggact aggagaaaat tcttatatac aagataaatt
    25741 tgttcgtcat gctgtaaaat gtgcagagaa tgcgaagttc tatggtatag gagcaggacc
    25801 aggagatggt tctttactta cgataaaagc ggttaatact ttaaagaaac tggatattct
    25861 atacactcca gaatcaaaaa agggtggaga tagtctagct ttatcaatag ttagtgaata
    25921 tttacctgaa tcagtggaaa taaagtctag gcatttccca atgagttttg atgggaatga
    25981 aaaaagttta gcttgggata atgtggcaca agaaattata tcagatgtta atgatggtaa
    26041 aaatgtagga tttgttacat taggagaccc tatgatttat agtacatatg tatatataat
    26101 gaaaagaata ttaggagagg tagaagtcga aactatacca ggaatttcat cattttccaa
    26161 tatagcatct aatcagaatt ttccattagt catggataaa gaagctttag tggtaattcc
    26221 ttgtactatg gaagatgata agatagagta tgctttgcaa aattataatt caatagtatt
    26281 gatgaaagta tataaaaatt ttaaagaaat aataaaaaaa ttagaaaagt atgatttaat
    26341 agaacatgct attttagtaa gtaattcttc ccaagaaagc gaaaatgtgt ttacagattt
    26401 aaaagaagcg caccttgaag ataagatatc ttatttttct actatactag taaataaaga
    26461 taataaaata aaataggtga aagcaatatt taaaggagac ttaatatgaa tatagtggtt
    26521 ggaactaggg gtagtaatct agcgcttata caaacagaat gggtaataaa tgaattaaaa
    26581 aagaagtatc cagaaattag ttttgagatt aaaatcataa agacaaaagg agatttaatt
    26641 caaaatgttt cactggataa aataggtgat aaaggattgt ttgtcaaaga aatagagcaa
    26701 caattattag atggaaaaat agacatagca gtacatagta tgaaagatat gccttcttat
    26761 ttagcagatg gactaaaatt tgctcatacg cctaagagag aagacccaag agatgttcta
    26821 atacttagag aaggatataa aaatttagat gatttacctc atggagcagt aataggaaca
    26881 ggaagtaaaa gaaggaagtt tcaattattg aaacaaagac ctgatttaaa tatagtacaa
    26941 gttagaggta atgtggaaac taggatacga aaaataaaag atgaaaatat gcatggtatt
    27001 gtgttggcag catcaggaat aattagagct aatttgcaag ataaaataag ttcttattta
    27061 cctgttgatg ttgttattcc agcacctgcc caaggagctt tagctataga aattagaagc
    27121 aatgatagta cgatagaggg aatagtaaat tcactgaagg atgagaatac agaaatccaa
    27181 atattggctg aaagaggttt tttagatggt gtaaatggaa gttgtcatat tccaatggca
    27241 gcttattgtg aaataatcca agataaaata catcttactg gcttatatgg agattcagaa
    27301 ggaaaaaaag tagtaatcaa gtctatagat ggagatattt cttctcctag agagttaggt
    27361 ttaaagttag ctaaacttgt attgaaggag tatgagaatt atgaagggta aggtttactt
    27421 agtaggggca ggaccaggtg attataaact catgacttta aaaggtttag agtgtataag
    27481 aaaatcggat gtaatagtat atgatagatt agcaaatagt agttatttaa gagaagctaa
    27541 gcctgattgt gaacttatat atgtaggcaa ggcatcaagt aatcatattt tgccacaaga
    27601 agatataaat agaattatag cagaaaaagc taaagaagga aaaattgtaa ctcgtcttaa
    27661 aggaggagac ccttatgtat ttggcagagg gggagaagaa gcagagactt tagtagatga
    27721 aggtatagaa tttgaagtgg ttccaggtat aacttctgct ataggaggtt tatgttatgc
    27781 gggtattcct ataacacata gagaccatgc ttcgtcattt catgtaataa caggtcattt
    27841 gaagggtgat gatagtggag aattaaactg gaatgctctt gcaaataaca aaggtactct
    27901 tgtgtttttg atgggaattt caaacctaga gaaaataagt gaaaacttaa tgaaagaagg
    27961 aaaagataag gatacacctg ttgctcttat aagttgggca actagatata atcaaagagt
    28021 tgttacatct acacttgaaa atgtttatga aactgctatt aaagaagaag taaaaccgcc
    28081 aactcttata gtagttggga gtgttgtagg acttagagaa aagttgaact tctttgaaag
    28141 taagccttta tttggaaaaa gtattgctgt aactaggtct agaaatcaaa atagtgtatt
    28201 ggtagaaaaa ataatggatt taggtggaaa tcctatagaa atacctacta ttaaggtaga
    28261 aaaaatacaa aataatatta acttagaaaa tgaaataaaa aatataaata agtataatta
    28321 tctaatacta actagtaaaa atgccgtaga aatcttcttt gaaaagatat atgaaatgaa
    28381 ttttgattta aggatattat ctaatttaaa ggtatgtgct ataggttctg ctacatcaaa
    28441 tgagttgaaa aaaagaggaa ttatagcgga tatagtacca aaaaaatttg tagcagagtc
    28501 actgtatgaa gagttagcgc ctattttaaa tgatgacgat aatatactta tacctagagc
    28561 agaaaatgct agagattatc tggttgacaa acttaaggct atttgtgaag taacagaagt
    28621 acatatatat agaacagtaa tagatgagga gaaaaaaggt gagatattag atattttaaa
    28681 ttctaatgac atagattata taacatttac tagttcctca acagtaaata atcttgttga
    28741 aattatagga aaagaaaata ttaaaatatt agaaaaaata aaaacactat ctataggtcc
    28801 tataactaca gagacaataa atagtttggg aataaaattg tacaaagaat ctgctacatc
    28861 tactatagat tcgttagtag atttaattat aaaaaattaa ttttggaggc ttgaatatgt
    28921 taagaagacc aagaagacta agagcaagta aagcaatcag aaatttggta agagaaacta
    28981 aggtgaatgt agaagactta atatatccac tgtttgtagt agagggggaa aatataaaag
    29041 aagaaattaa atctttacca gatgtctatc atttatcaat agatatgcta gaagacgaaa
    29101 taaaagaaat aagtgagttg ggaattgaac atataatgtt atttggtgtt ccagatgaaa
    29161 aagatgagtg tgcttcacaa gcttttaatg ataatggaat agttcaaaag gcaattagaa
    29221 aggtaaaaga aataaattca gatataaatg taataacaga tgtctgtatg tgccaatata
    29281 caagtcatgg acattgtggg attttaactg atactggtta tgttgataat gataaatcat
    29341 tggagtattt ggctaaaata tctgtaagcc atgctaaagc aggagctgat atggttgctc
    29401 catctgatat gatggatgga agaatacaag caataagaga agctttagat gaaaatggat
    29461 tagaaaatgt tggaatcatg gcttatagtg ttaagtatgc ttcaaattat tatggacctt
    29521 ttagagaggc agctcattca gcaccatctt ttggagatag aaaaacatat caaatggacc
    29581 cagcaaatag caatgaagct ataatagaga ctgaacttga tatattggaa ggtgcagata
    29641 tacttatggt taaacctgca ttatcatatt tagatattat aagaagagtt aaagataatt
    29701 ttgacttacc tctagcagct tataatgtaa gtggagaata ctctatgctt aagtttgctg
    29761 tgaaggaagg tttattaaat gaagatgtaa ttcttgaatc aattacatct ataaaaagag
    29821 caggagcaga tataataata acttattttg ctaaagacat tgctaaactg ttgaaaaaat
    29881 aaattctagt agtataaaga atatcaaaaa gtataggaat tgtaattaac tattatacag
    29941 tagttttgtt tagacattta ttgaagaata atagaaacta atttgcaatt tctatacttt
    30001 atttatgtaa ctaaataaaa tttggaggta gaagatgcta tatcctatta atttaaaatt
    30061 agatgagtta gatgtagtta taattggtgg tggagaagtt gcatatagga aatgtaaaaa
    30121 ttttttagag tttaacaaaa atgttactat agtttctaaa caaattttaa ataaatttta
    30181 tgatttaaaa ggaaatataa aaataataaa agatgattat aaagaaatat atataaaaga
    30241 tgcttctgta ataattgctg ccacaaataa tagagagtta aatatggaaa taggattata
    30301 ttgtaaagca aaaaataagc tagtgaatgt agttgataat atagaaatat cgaactttac
    30361 tgttccatca tatataaaaa gaggagattt acttataagt gtgtctactg ggggcaagag
    30421 tccttcatta tcatcaaaga taaaaaaaga aattgaagag agatatactg aagattatga
    30481 ggagtattta aatgtattag gagatataag aaaaaaggtt ataaaaaaat acgaagataa
    30541 aagtaaaaga aaagatgtgc ttaatatgtt ggtaacactt gacctagaag aacttaagaa
    30601 gtttgatata taaaaaataa aataaaaaat gttaaaaagg tgttgacgga ttatttttaa
    30661 g
//
LOCUS       DAENCV010000040        30505 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAENCV010000040 DAENCV010000000
VERSION     DAENCV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.040"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..878)
                     /locus_tag="KQD82_001799"
     CDS             complement(<1..878)
                     /locus_tag="KQD82_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG4380273.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLDHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPD"
     gene            complement(988..1902)
                     /gene="murQ"
                     /locus_tag="KQD82_001800"
     CDS             complement(988..1902)
                     /gene="murQ"
                     /locus_tag="KQD82_001800"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG4380274.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(2001..3098)
                     /locus_tag="KQD82_001801"
     CDS             complement(2001..3098)
                     /locus_tag="KQD82_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG4380275.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLKH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(3331..4677)
                     /locus_tag="KQD82_001802"
     CDS             complement(3331..4677)
                     /locus_tag="KQD82_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380276.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(4766..5074)
                     /locus_tag="KQD82_001803"
     CDS             complement(4766..5074)
                     /locus_tag="KQD82_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380277.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(5336..5734)
                     /locus_tag="KQD82_001804"
     CDS             complement(5336..5734)
                     /locus_tag="KQD82_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380278.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKEMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(5736..6896)
                     /locus_tag="KQD82_001805"
     CDS             complement(5736..6896)
                     /locus_tag="KQD82_001805"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG4380279.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREIFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(7327..8346)
                     /locus_tag="KQD82_001806"
     CDS             complement(7327..8346)
                     /locus_tag="KQD82_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4380280.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNIEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(8340..9020)
                     /locus_tag="KQD82_001807"
     CDS             complement(8340..9020)
                     /locus_tag="KQD82_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380281.1"
                     /translation="MYRILLVEDDIDLSKEIALAFEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(9172..11079)
                     /locus_tag="KQD82_001808"
     CDS             complement(9172..11079)
                     /locus_tag="KQD82_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4380282.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AVLKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(11080..11832)
                     /locus_tag="KQD82_001809"
     CDS             complement(11080..11832)
                     /locus_tag="KQD82_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380283.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(12148..13302)
                     /locus_tag="KQD82_001810"
     CDS             complement(12148..13302)
                     /locus_tag="KQD82_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4380284.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSVLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(13305..13772)
                     /locus_tag="KQD82_001811"
     CDS             complement(13305..13772)
                     /locus_tag="KQD82_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4380285.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(13786..14274)
                     /locus_tag="KQD82_001812"
     CDS             complement(13786..14274)
                     /locus_tag="KQD82_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380286.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(14288..15619)
                     /locus_tag="KQD82_001813"
     CDS             complement(14288..15619)
                     /locus_tag="KQD82_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG4380287.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKVQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(15633..17198)
                     /locus_tag="KQD82_001814"
     CDS             complement(15633..17198)
                     /locus_tag="KQD82_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4380288.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(17367..18152)
                     /locus_tag="KQD82_001815"
     CDS             complement(17367..18152)
                     /locus_tag="KQD82_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4380289.1"
                     /translation="MRLEEMIFFNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(18319..19518)
                     /locus_tag="KQD82_001816"
     CDS             complement(18319..19518)
                     /locus_tag="KQD82_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG4380290.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDIKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(20041..21378)
                     /gene="xylA"
                     /locus_tag="KQD82_001817"
     CDS             complement(20041..21378)
                     /gene="xylA"
                     /locus_tag="KQD82_001817"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG4380291.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(21474..23024)
                     /gene="xylB"
                     /locus_tag="KQD82_001818"
     CDS             complement(21474..23024)
                     /gene="xylB"
                     /locus_tag="KQD82_001818"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG4380292.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            23232..24392
                     /locus_tag="KQD82_001819"
     CDS             23232..24392
                     /locus_tag="KQD82_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG4380293.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDVGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(24548..24952)
                     /locus_tag="KQD82_001820"
     CDS             complement(24548..24952)
                     /locus_tag="KQD82_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380294.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(24973..25455)
                     /locus_tag="KQD82_001821"
     CDS             complement(24973..25455)
                     /locus_tag="KQD82_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380295.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(25475..26284)
                     /locus_tag="KQD82_001822"
     CDS             complement(25475..26284)
                     /locus_tag="KQD82_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG4380296.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(26277..27056)
                     /locus_tag="KQD82_001823"
     CDS             complement(26277..27056)
                     /locus_tag="KQD82_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380297.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(27056..29134)
                     /locus_tag="KQD82_001824"
     CDS             complement(27056..29134)
                     /locus_tag="KQD82_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG4380298.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(29933..30454)
                     /locus_tag="KQD82_001825"
     CDS             complement(29933..30454)
                     /locus_tag="KQD82_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG4380299.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIHHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
BASE COUNT        10081 a         5427 c         3156 g        11841 t
ORIGIN      
        1 aaatctggag tcaacactgg tctcactata ccaaatctcg ttttattcaa atatttttta
       61 agtggctcta atattaaatc tcctgactta aacactccac ctgaataaga tacctttatc
      121 tttgtacctt gttcaaaatg atgatctaat cctattataa gcctacttaa ttcataagct
      181 gcatcatcaa atattttact agcagtatta tcaccttcaa taactgcttt taaacatatc
      241 gtagcaaact ttgctatttc tattctatct ccttgtattt catcatttac atattttatt
      301 atgtcataat cattctctat attaagatgt ctttttatta aatcatataa ttttgttttt
      361 gaacaccttc catcagactg cattgtgaac atttttaagg tagccattcc tatataatac
      421 gctgaacctt catctcctaa tacatagccc catcctccac atctgtgaag attcccatct
      481 ttatctaaag cctgtgctat tgaaccagta cctgcaatta tatttatacc tttttcccca
      541 tttaaagaac ctgctaatgc tatttctaca tcattaccta gagtataatt tatatgacta
      601 tatgcatttt ttgttgctac ttctaatgca tacaatactt cttttatttt tccatatcca
      661 gcaagtccaa caaatgcata cgttatttct tctacattta tcttagcatc tttacaaact
      721 tcttccaacc cagttattaa taatttcgtt agattatcaa atcctatttg attataatga
      781 caagttcctt tggttatggt tcctactata tttaattctt catctactaa tgtaaatgtt
      841 gttttcgttc ctccaccatc tacacctaaa aaatacatac tatcctccta caatatcttt
      901 aataattctt tcaatactta atatctaaaa ataaccttcc aatgctgttt ttttaatatt
      961 gaaaggttat cagtactatt atttatttta caatttttct attgatttta aagcttcact
     1021 tatataacct tcttgtctat ctaggatact ctttgcacta tccttatcta aaccactttt
     1081 tatcataaat atagccaatt taacgctatt atctgcttct tctaagaatt tttctgcaac
     1141 gtcatatttt acatttgtag ctatcattat tattcttttt gctctttcaa ccaacttttc
     1201 atttgatgtc ttaacatcta ccatcaagtt tgagtatact tttcccatct taaccataga
     1261 agctgttgtt aacatattac ataccatttt ttgagctgta ccacttttca ttctagttga
     1321 gcccattatt acttctgccc ctactataat gtttattggt aaatccgctt ttttactcat
     1381 ttcactattc ggattcatag ttattgatag tactgcacac cccaattcct tagcatactc
     1441 cattgcacct ataacataag gagtccttcc tgatgcagct ataccacata taacatcctt
     1501 agatgtaact cctctatcta ttatatcttg ctttccaagt tctttatcat cttcagcacc
     1561 ttctattgct ttaaatattg cttgatttcc accagctatt atagcttgaa caagctcaaa
     1621 atccactccg taagttggag gacattctgt ggcatctagt attcctatcc tcccactggt
     1681 acctgctcct atatagaata atctacctcc ctgcttcatt ctatctacta ttatattaac
     1741 agcttttgct atactctcct tttgttcttc aacagcatag gctacagttt tatcttcgtt
     1801 atttattaat tctattatgc ctaatgtatc ttctttatct atttgcaatg tattcttatt
     1861 tctaccttct gtaactaaat tttctaaagt ttttattaac atatcatttc ctcattttct
     1921 taatattaaa tttttaaaca cattttaatt ccaactttca taattatatt tttataataa
     1981 attaatattt tcataataaa ttattttgtt gagggtttaa aattaaactt ttgccatggt
     2041 ttaatataat ctaatataaa taattcttct tttcttatat atcctactac attcgattta
     2101 ccactgtttt tcatatcagt caaggctatt tgtaactccc ctgcataatg accatattca
     2161 ctactttcaa ttactatatc tcctcttttt attattttag gagtattaaa tagttcaaat
     2221 ttgtatcctt tatatttaac acgagattga gtagacctta tcagactatc agatatgtcc
     2281 cctctattaa aatgtatttc ttcaaagagt atcttcttct caacttctcg tatattttcc
     2341 tctattgcta catcaaatgt aaccatatct tttctcatag aacctaatac ctttagctcc
     2401 tcatatgttg gataacaatt tgatattatt atatcattta tattattcat agctattata
     2461 tgctttactt gaatatctat aggtagattt ctgtgcatct caattgttgg aagcccatca
     2521 gttacaggcc atggtccaaa tgtagtttca ttttgacttg ttacaaaaac tgctgttctt
     2581 aatccatact tattaaatct ttctgtacat tttataaagt gttttaaatt taatccacta
     2641 tatctatgtg gataaaagtt atgacatcct atcaaatttt ctttatttgg ttggtagtcc
     2701 attattgtat ctatataatt agtattatta ctcatattga tttctatttt aagattttga
     2761 ggattaaaag tcattaatgc ctcttcagaa cctgtaaatc ccatatcaag ccttattccg
     2821 tctgccttcg tctttttaaa aaaagtcaaa tctttgtagc ttatttctaa ttcaccaaat
     2881 actctgggat ttacatcaag tattacctca aaacctttaa acttagcgta ttcatttata
     2941 cgtttaaatt ttttaattat attttcttta gtatctttaa ctgataataa acatgaaaat
     3001 atacgtgtaa atccattttc agcagcttta tctatataat catatatttc cttagatgtt
     3061 gttttttctg gataaattga tattcctaat cttcccatct gtgtcatctc cttatatcct
     3121 atatgtgttt aaatttattt cgaattacac gaaacttttt taaaaatttg gtgaaaaaaa
     3181 gtttcatata tatttataaa aaaagaaaag tttacctttt ctttttttat aaacaatcaa
     3241 caaaatatct tcgttactta tttcataact gttatacttt gaaatttata ctaatctaat
     3301 atagttttta atttccaata tagtttctaa ttaataagct tcttgctctt caactttttc
     3361 ttcatgaagc aatctcttat cgtacatctt gacaaatgga tagtaaatta ttattgatat
     3421 agctatacat aataagttaa gaactgaagc tctccaatct ccaccagtgg ctaaatatgc
     3481 ccctactggt cctggtaatg tccaaggagc agtaactact actctggata ctaaattagc
     3541 actcatagca aaccatgaaa ctgttgccat tactattggt gctacaacaa atggtattat
     3601 taatattggg tttaatacta ttggtgctcc aaatattatt ggttcattta tattgaatat
     3661 tgctggtatt acagttgctt ttcctaaaga ctttccataa gctgattttg ctctaaatgc
     3721 aagaagtaaa gctaagccta ttgtagctcc agaacctcca atccatataa accattgaaa
     3781 aaagggttca gcagcaatac ctggaactgc ttgacccgca gcatatgaag ctgtattagc
     3841 atctaataat tgtatccata aaggtcttgc caatgaacct attattgata caccatgtat
     3901 tccaaatgac caaaaaaacg ttattaaaaa tattagtata attactgatg gtaatgtgtc
     3961 agtggctgaa actaatggag ttactaattt agctatgaag ccatgccaat caaaccctag
     4021 gaaataagta acaatagcca ttaaaattat tattaatgcg gctggtgtca gagcttcaaa
     4081 agaccttgtg actgatgctg gaactgcttc tggcatggtt atcttgaatt tagatttgtc
     4141 agtaaatcta taaatctcaa cacctattat agatgttaca atagcaacaa acattgaacc
     4201 tccaccaaga ttagccattg gcatttgcca tccttgagga attgaattat accaagctaa
     4261 aagttctgga ctcttttcaa ctatctttaa taaatcaggg gtaagaccct ctattgcagt
     4321 aggaactatt gtcaaaagaa atgtagctgt agctaataaa cctcctgtca atccatctaa
     4381 cttatatgac tttgccaaac tataacctat gccaaatgta gaatatactg atataataaa
     4441 aaatgtcatt ctatatggta gtaatattgt aactgcattt tcctttagat actgagttac
     4501 cccccatgaa tctggtaatg gaggaaatgc taatatcaag aaaaatgacc caactattat
     4561 aagtggtaat gaagctacga ttccatctct tacagctctt aagtgacgtt gttctgccaa
     4621 tctggccata gaagttgata atttagtgtc taaaaattct gtaaatttat ctagcatctt
     4681 ttctctcctt aattaatatt tatttttatt atcattaaaa tttttataaa atcaactttc
     4741 ttctaatcta acaacactgc aactactatt tactcaacag ttcttgaact tgttttaata
     4801 actttggtcc tcctaatgga ctataagctt gtgcttgtat taatgcacat ggtacactaa
     4861 cctcatcagc aaaagctttt aaatagtcaa atctatgtct gacttgtgga gctaccatag
     4921 caatatccca tccattttta acttcatcct caaactcttg agtaccaaca gccaaaacat
     4981 ctatatctat tccatttttt gctccttctt tttgtaatgc attaactgct atagcacttg
     5041 acatccctga tgaacaaaca aataatactc tcatattgat tccccctgtt tttttatttc
     5101 ttttcaatca ttatagcatg tatttttgat tacaggaata cgtttgtata tattttttca
     5161 tattttacat ttttatttgt aattttattt catgaattta tatcttgctt taattgcttt
     5221 atttatctaa tcattctttt gtctaaaatt acacactact taaaatactc atatcttttc
     5281 ttaaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata cttttttaaa
     5341 tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa gcaaataaaa
     5401 tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat
     5461 atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat ctattatcta
     5521 ttccaaatat agataataac aaaggaaaaa ctacagtact tacaagagat gaaactatta
     5581 gtattaatcc acttttaaaa cttatagttg gaatattata atctgttctt tgatttttct
     5641 ccatttcttt cttagtattt ttattacttt ttgtactttt tgcattttta taattattat
     5701 ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt
     5761 gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa
     5821 gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa
     5881 gaatacccat attcatcttg agttaatact tcaacctcta ataataactc ttttatatat
     5941 cctattaaag ttagattatg agctcctcca cctccaatta ataaagtatc tacattaacc
     6001 tttggaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact
     6061 gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaatag
     6121 acttgtccaa atatctctct tccagtgctt ttaggtggcg cttgactaag atacttatga
     6181 ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt
     6241 ttatcatact ttctattaaa taatctttga catactccat ctattatcat atttcctggt
     6301 cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt
     6361 cctattccac caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat
     6421 tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa
     6481 ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc
     6541 gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat
     6601 gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct
     6661 ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc
     6721 tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc tagagttata
     6781 aattctattt gcttaacttt agtatttcta ccattcccac taatttcagc tagtactgca
     6841 tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt
     6901 ttaagtttat agttattcct ccccaaatca aggtattaag tttaataatt ttagaattaa
     6961 tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag ttaatcttat
     7021 acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatacc
     7081 atgtaatttt ttttcacaaa caaaacatat tctctctcca aaaaccatat taaaatagtt
     7141 actacaaata aaaaacatac caggtacata tctgatatgt ttttcatata taataattat
     7201 tattttataa tatagataga gcatcattta aggcactaat taaatcttca ctattttcaa
     7261 gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct
     7321 aataacctaa ctatctaggc ttaaataatc tgatatatca taaaaataga tattaaaaat
     7381 tgtatatgaa ccattttccg aacatacctc aatctcatgc cctaaactat ctatcatttt
     7441 ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc
     7501 cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta taccaaaatc
     7561 ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac
     7621 ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg acatgcattt
     7681 agaatctgtg taaacatcat tttctaattc attcaaacta acttctatat tattttttat
     7741 aagcaaaaat gaattatttt ttatagacat ttttaccaat ttttctaaat taaatttatt
     7801 tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa
     7861 aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct
     7921 atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat
     7981 gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat tattttttat
     8041 ttttaagttt tctataatat cttttattag attttcttca aaagattgtc catcaactaa
     8101 ataactgtct atttcaccac tattttctat acaattatat aaatttgtgt aactatttct
     8161 aaaatttata taatctataa caaaaaatac cattattata aacagcacta aaaaatctaa
     8221 atatattaac tctgaatatt tatttttaaa aatatctata gcaaataagt agatatttat
     8281 tatactaact acaactaaag gtagagcaat atacttagac ctacctttca aatattttac
     8341 tagcattgta ttatatatcc tatccccttc ttagttttta taaaatcatc aaaacctagt
     8401 tctttaactt tacttctcaa tctattcata tttacagtaa gtgcattgtc tgtcacaaac
     8461 tcatcattat cccatagagt catcataaga ctttcccttg atacaaccct attcttattt
     8521 tttaataata atgttaaaat ttttatttcg tttttagtca actctatact tttatcctta
     8581 tacttatgtt ttataatccc tttccccaca tcaaacatca aatcttcaca atacaaaata
     8641 ctattagaat cactataatt atagactctt cttattatac catttatttt ggtaaccaat
     8701 atttctatag aaaatggttt ggtaatgtaa tcatctgctc cattatttat acccattatt
     8761 aaatccatat cagaatctct tgatgataaa aatattaatg gtacatttga aatagctctt
     8821 attttttcgc accagtaaaa tccattatat aatggtaaat ttacatctaa aagtactaca
     8881 tctggttttt tatctataaa ttcatctaat actacctcaa aatcatcaat taattctact
     8941 ttaaatcccc atttttcaaa tgctaatgca atctcttttg ataaatcaat atcgtcctct
     9001 actaataaaa ttctatacat ttgtatattc tccattatcc taatatttca ctatgctatg
     9061 aattattaat atcataacat attttatatg aatttattac ccaaattatt tagttaatca
     9121 acaaggagac tcttattaca agaatctcct ttctctttat taatatatca actaattttt
     9181 atacactaca ctcttaaact tattaacaga tactgctcca tatattatga atattatcaa
     9241 acatatcact aaacttagta tatatatacc aaataaatcg gtcatcaata aatctcctgc
     9301 taatctcaat gcaaaataac tagatatagt tgcacttaac attggcaaag tataaaaaat
     9361 agctacttct ttaagtacag ctctatttat atctttttct gtagtaccta tttttaacaa
     9421 aatcttatat ttctctttat cttcatatgc atcactatat attttcatat atattatact
     9481 tccagctaca attgcaaaga ctaagaataa aaacacaagc actacatatg caaacttcat
     9541 ccattgactc tcaccttgag ctttatatcc attttgaata tacacatttt ttaaacttaa
     9601 attcttttca atattgttga acattacttg actattttct tcttgaaata cctttgcacc
     9661 ataaaaatac acttgttgac ccttatctct tagtttaaaa tatgtatcat catttaccac
     9721 tataatactt tgattaagtc ctgtccctag tactggaact tttacttctc cccttgaaat
     9781 attatataac tgaccatcaa gttttatctt ggcttctttt gtagctattg atgctatcgt
     9841 ttgagggctt tttaactcta ttacattatt tccactaact aatctttcac catactcttt
     9901 taattcttta tcattgccat tcactttaag agtattacaa aaatcactat atttcattat
     9961 agtatctgtt gtatcataag cttcttttat atctgttttc tttaaaattg ttagttctgg
    10021 atttactgag tactttatct tattagtatc ttttattata ttctctattt gttttttatt
    10081 cacttcatct ttatccattg aaactatgct aatagtgtac acatttcttt gttcttgggc
    10141 attcttatgt atacgcttca tcgcaactga agcaccaaga gttgatattg ttgtagttat
    10201 aattatagct attatagcat aagttttata gtttttattt aatcggtagt ttatattgtt
    10261 tattgcaatt atattatttc cattaaataa tattttttta tttttgatta taatatcaaa
    10321 tataacaggc ataactgatt tgaataatcc ataagtacct actattatta tacctataac
    10381 aggtagtaca ctagttatat ctccagttct tatattggta gaaagatagt agccatatcc
    10441 tattactata attgatacta tagctaatat atatatacct attcctacct ttggtatttt
    10501 ttcttgtttt ttagatatat tgattagatt tattacacta cttctacata ttgttataaa
    10561 accttttaca gccattatta taaatataga aataaagatt aaagtagcat atagtatagc
    10621 ttctatacta agtccattag atacctttat attaaatcca gataacttta ttatcactac
    10681 ttgaaaaaat ctagatgtta ttatccctat aattattcct gttatacaag aacttattcc
    10741 tataagcata ttttctacaa aatatatttt tcctatagta tataaatcta atcccataaa
    10801 agacaatatt ccaatttctt tagacctatt cttaaaaaat atatttgttg cataccatat
    10861 aaagaaaaat aaaaatactg atagtatcat tattacagct actaacactg atagtgtaaa
    10921 ttttttattt tcatcttgta acacatttat agcatcacca tatcttaata attcaaaatt
    10981 agttagcaca actactgaaa aaaccataga tataaaaaat gctagataag ttcttatgtt
    11041 actcttaaaa ttattataag caattccaag tatactcact tacatctcac ctcccataga
    11101 ggctagcata tccataattt ttccataaaa ttcttttcta ctatttcctt tgtttaattt
    11161 acactttata tttccatcat ttatcatata tacttcattt gaataacttg ctgttagagg
    11221 gtcatgtgta atcattataa tagtagcgtt attttctctg tttatttttt ctaatgattc
    11281 taaaagttca tatgctgatt tagagtctaa agcaccagtt ggctcatcag caaatatgac
    11341 tgttggattt gtaattaatg acctagctgc tgcgcctctt tgtttttgtc ctcctgataa
    11401 ctgatatgga tatttatcta aatgattttc taatccaaat tttttagcta tagaaaatac
    11461 cttagcttct atctccttac ttttcttttt tcctaatgct agaggaagtg ctatattgtc
    11521 cattagtttc atattgtcta atagattata gtcttgaaat ataaacccta ttttttctct
    11581 tcttaatttt gatagttcat tattttttac ttttgatata ttaattccat ctagtattat
    11641 ttctcctgaa gtttgtttgt caagtgtaga cattaaattt agcaatgttg tctttcctgc
    11701 accagatgga cccattattg atataaaatc accttcattg actgtaagag atatattttt
    11761 taatacattt gttttaaatc cttttattcc ataatctttt gatatatttt ttatttttaa
    11821 tatttctttc ataataatag cctccagttg ctaatttttt aatatatatt tattttaaag
    11881 gttttatata aataaatcct taagtaaata ttacatttta actgttagca ttacactttt
    11941 gtaacatatg gaagactatt ataattataa tggaaatatc ttaaaataaa agcatgattt
    12001 acttctttgt ataaacaaag aaaagaagct cctaagaagc ctctaatcta tacaattatt
    12061 cttctctata aaaaaccaaa atacacattg attgtggcaa atacagatac tataaacaag
    12121 tgatattact actatatatt aaaacaatta ttcctgaaga taattcaata agactatcaa
    12181 gcaataagct aagcatccaa aaagacttaa taccattaat gtataattat acccaatatt
    12241 aatgcttaaa aaaccagcaa cagttggaat aatcagagat gaacctccca taaatatact
    12301 taaagctatt aattggctac tacttctaag ttttttagga gaaacttcaa atattaatct
    12361 ttttgaagta attaaaatta aaggagtaga aaacacttga aaaacacaca gtataataat
    12421 gatatttgaa tcatttgtta aagcaaatag tagaaactga actgttatca taatagcact
    12481 taattttaat aagtttaaac tgctatattt ttttaataaa taagaaccag ccagatatgt
    12541 tggtatctct aacactgact gtaaagacca ctttaaagat atctgagaat ttgtagcatt
    12601 taaagctatc attttatcta ttacagtaca gttatttgct accaccattg agtataataa
    12661 gaataaaata catactagta agatatattt tttatctttt aataattcaa taatatcttt
    12721 attagttaca tttattttct tattaatctt ttcaatatca ggtaataata gtatatttat
    12781 aataagtaat actacaatta aaccaatcgc tacagataat ccaaaatatg agaatctaag
    12841 aatcaagtaa gatgctacca tacttccaat agcccatcca atcgacccaa aagtcttaat
    12901 aaatgacaaa taattattaa tattatctcc acaacctatt atccatgtat catataaact
    12961 acagcaagta tttaataatc ctcctgataa agcaacaagt aataagtata ttataaaagt
    13021 tgcagattca gtagaaaaca taataactga tgcaattcca taaactgata aactaattaa
    13081 aacaattttt ttcattgttt gatacttatc agctaatact ccaaataata attgaaacaa
    13141 tatattggtt attgcatagc tcgataatat aattcctctt tcaaacgctg agtaacctaa
    13201 ttttgataag aaaggtgtga attttgttga agcaaaggca aaaataatat atgagaaaaa
    13261 atagaatatg ataaatttaa atttaatcgc tttattaatc ataatcaatc aaaattgata
    13321 actttaatac cactatttcc ttcctgattt aagtttgaaa gatgatattt taccgtatta
    13381 ggattctcta caataactaa aggaatatat aaagtatttt caagtaactt attatcaatt
    13441 tctactaact tttcccccat attaacttct tgaccttgtt taacttctaa tttaaagttt
    13501 tctttttttg ttgttgcact atccaatcca atatgaataa gaatttgaac atcattttgc
    13561 aatttaattc ctatagcatg gttagtttga gcaataaaac ttattactcc tttagtcggt
    13621 gatttaagaa tattgtcctt tgctatcata gcaaatccat ctcccattac cttagttgaa
    13681 aatactttat caggaatttc tccaagatta attatttttc catcaatgta ggaattaatt
    13741 gtaattgctt ctttttttct tttaaagaac attatagaca ctcctctaca taattaactt
    13801 tattctaatt atcattacaa ataacgatat taagcatact gcaattgagg aaaaaagagc
    13861 cttatagctc tttccccatt gtagttcttt aatactgctt atgatttgtt taattccaac
    13921 aaatttataa atataggtaa acatgataac aatagttatt gtgtaaattt ggaatatctg
    13981 ccctaataag actaaataga aagcaatcat gttttctttt atctctttat tcttttttat
    14041 attgtaaata acatatgcac tgtatactgc actaaagtta gagattaaat ttaaacttgc
    14101 attaacatca ttgtttaaaa gttgtgaaaa tgaaatattt gtaaataatg atgtcaattc
    14161 taaatacact atatataaaa caggtaatat tagaaaacaa accataacca ctgaaagaaa
    14221 tatattttca tcttttaata ctgctgtgat attttttatt ttatttgtta tcatgttttt
    14281 gttctcctta tttaaaagtt ggccagaaat ccttattagc cacaatcatt tcatctaaga
    14341 tttcttttgc tacttttgca cttggaacag ttttactcat agtaaacgac tgccataatt
    14401 tttgataact tttttctatc catgcttcta cagctaattt ttcagaagca acttgttgtt
    14461 ccattaaacc tttttggaaa gttggaattt tacctataga cataggttca accatatctt
    14521 ttccaacaag gcaaggaatt tcaaccattg catcatctgc aaaattttca atagctccat
    14581 tattaggaac aatcagtaaa aatctttcct gagtattaaa tgctaatgca caagctaatt
    14641 caacaataaa ttgagaatgt tcacttgcat caaaccatgt atctcttgct gtgccatttt
    14701 ttataattct ttcacattct tcgaatgtat ccttttctct tctatccata atttcatttg
    14761 ctcttgtata atttggatta gcatgttcaa cagtttcatc agccattaaa taatatttta
    14821 aataagtatt aggtaatgta gtcggttcta tttccaataa atcctttgct gctctcatag
    14881 tttctaacca actagattct ttgtgttgac cttgaggtgt aaattcctca tatccttttt
    14941 tagcacaata ttcttgtact ttaggcatta aatcatttcc ttctttgtcc ttaatacttg
    15001 tccaccaccc gaaatgattc aatccataat atctaacatc aaagtcttta cgagaattta
    15061 aacctaaaac tctagcaaat atttcctcta aacatactgg catatcacaa atattaataa
    15121 ttcttgaatt tggtcttaat acacgacatg cttctgcaac tatagctgct ggatttgaat
    15181 aattaagcat ccaagcattt ggagaatatt tttccatata atcaatcatt tcgattattg
    15241 gacctattgt tcttaatcca taggcaattc cacctgcccc acaagtttct tgaccaacaa
    15301 ccccatattt tagtggaatt ttttcatctt gttcacgtaa agcatataaa ccaacacgaa
    15361 tttgtgccat tacaaaatca atatcagtaa atgcttcttc tggatttgtt gtataactaa
    15421 atttaatttc tggatttttc tctctaacaa ttattgcaca tgcttcagca acttttcttt
    15481 gtctctcatc attattgtca tataatttaa ttgattcaat aggaaattta gcaacacttt
    15541 caattaacat tgttacaatt ccagcagtat aagtactacc cccaccagca attagtatcc
    15601 tctgtctttt tctttccatc tttccttctc ctttataata aattttcaaa ttcttctctt
    15661 acacttggta ctgaaagccc aataataatc tgaatagctt ttccattatg cactaaacca
    15721 tgtgcaccta ctgccttaaa atcttcttct ttagaaataa ttgagtcatc tttcaaggtt
    15781 aatctcaatc tcgtagcaca gtttgtaaca tctacaacat tttctgctcc accaactaat
    15841 tttatgtatt cagatgcttg ttgagaggct ttactcaatt tatttccttt ttcatcttta
    15901 gtttctaaat attcattttt agtatatagt tttgtttctt cactatcttc ctctctacca
    15961 ggagttttca tatcaaactt cttaattaag aaagtaaata caaagaacca gataaatgaa
    16021 aatataatcc ctacaacaac ttgaacaaca tataccttcc agtgatacat tccaagagga
    16081 agccaattta atacaaccca gttaataatt cctccaccaa aatcaccaac aactcctagg
    16141 gcaaacgaaa tcgtttgcaa gcatgcatct aaaaatgcat gtactaggaa taaaagtggt
    16201 gctacaaata aaaatgtaaa ttctaacggc tcagtaattc cagttaatac tgcagtaaga
    16261 gtagcaggaa tcattaaacc taatactttt ttcttttttt ctggctttgc agttacatag
    16321 aaagcaccaa atacacctaa tgaaccaaac accttcgata atccggataa tgaaaatccg
    16381 attggataca tttcctttag agtcttagct gaagtttgaa agtcctgaat atgagtagcc
    16441 caatatactg atgtaccacc tggaacaaca acactatcat aacaaattgg tgcatataca
    16501 aaatgatgta atccagttgg tattaaaatc ttttgtaaga atgcataaca ccaaactcct
    16561 atatttccac tatttataaa aaacgtttgc aaatgtctaa taacattttg aatatgaggc
    16621 caaataaaac aagttaataa tgcaactggt atcataacaa agaaacaaat tgcaactaca
    16681 agtgatgagc ctctaaataa tccaataaaa tctggcagtt ctttatcata aaatctatta
    16741 tgaatataaa tgacaatacc tgatattaac aaagcaccaa ccataccagt atctaatgtt
    16801 ttaataccag caatgcttac aagtccactt ccagcactta tttcagctgc gaaatcaact
    16861 ccaaagcttt ttccccaaaa acttaacata gaacctacaa aataattaaa agtcaaatac
    16921 gttgctaaag cctccataca agctcttgct tgttgctttt ttgcaagtgc tattggtaaa
    16981 gaaatagcaa ataataatgg taattgatta aataccatcc aagctccact agatattaca
    17041 ccccaaaatt ggtaccaaat attttctggt gtagcaagtg aacccataat tgactgattt
    17101 tgaaatacaa ttgagaatcc aaccattatc ccagcaaaag caaataatag tgttggtgta
    17161 aacattgccc ctccaaaacg ttgtattttc ttcatcatat aaattactcc cctcctcata
    17221 tttttagttt atctttggcc ttagatatct attattttaa caggttaata aatatataca
    17281 atctttaggg tatattcgga gcttattcac agtcaaataa gtttttgcac atttttatgt
    17341 tcaaattctc ataaaaaaaa gaatgactac aatttgcaat caatcccttc tgtaatcatt
    17401 ctatgccttt tataaatact atatttaata aataataact caatcaatat aaaatataac
    17461 attgtcaatt tataactagg gtgcatttgt aaaaatgaga tattagtcgc tgaaatataa
    17521 attgattcat cacttaaatc agtcaatgta ttatttttaa actctgtaat tgaaattatt
    17581 ttagttccta ttaattttaa tcttctagca atattaagtg tattctcatt ctcaccactt
    17641 agggatataa taatcataac atcattttta ctgacaatat tgtaaaaaga atcatcaaat
    17701 gttcttccct caaaatcata tattatttct tgacatgata agaacattct ctttatctgt
    17761 tttgcaacca cattttgaat atcacctgaa ccataagcgt atattgttct tgattcataa
    17821 agaagtttac acaatgcatc ataatcttta tttttttgat cttcaacata cttaattatt
    17881 gaattacatg caatatcaaa tgactgattt tctatgacat gaaccttttc tgattcccag
    17941 cttaataata ctttaaactc actgaaacct tttaaatcta attttttaac aaatcttgta
    18001 atagttgcac gagacacatt acatttcata gcaagttcat ttattgacat cttactacaa
    18061 tgttttttat ttgcagttat atatttccat attaataaat ctgtatcatt caatttatta
    18121 taattaaaaa aaatcatttc ttctaatctc ataaactatt acccccttta attaaaaatc
    18181 gtcataaata ttataccaat attcaacatt aaattacagc gtattcttat atttactatt
    18241 tagcccataa aattgactat aaaaaatact gctataattg atgtttaaaa aaatgtagtt
    18301 ttaattttat tcttttatct aataattaat attttcaagt tctagtgctt ttcttataca
    18361 attatcatct ttaaaatcag cagtcctttt acaatctttt actttaaatt ctggaatagt
    18421 tttaaattcc tcatcacata ttccattctt atttaaatac atacaacttg ccattctaac
    18481 tattagacca ctatttttta aattaaacaa tacagggtct atacaccctc catctccgcc
    18541 agtagtttca ccaactaaag tcgcaaattt attttcttta caaaacattg caaacgactc
    18601 tgatgatgaa tatatttttc tatcaacaag taagtatata ttacccttaa attttgaatc
    18661 atctgtagat tttatagtat aggaagtatc ttcaaaatca ttaaaatctt ttataatttc
    18721 tttaggtgca ttttccttta cacttgctgg taaattttct acaggattta attttatatt
    18781 tcttgcattt gtatattttt ttactatttc attattattt ctataaattc tataaccatt
    18841 tattttgata tcatttttta tgagtttaga tacgattccc tgccaatacc tatcacttcc
    18901 acctaaatta cctcttatat caataattaa agctttatgt ttttctaaag tgttaatata
    18961 atctccaatt atttttaaat cattatttat agagccattc tttgatgaca ttgatggtaa
    19021 atacatatat ccaatctgac ccttaataac atcctttaaa attaattctt tttttacaaa
    19081 aatatcatct ggcattttta ttttaagatt catagagtca tatctatcca caactttttt
    19141 atcattaaga aaatcatacc aatcattatt agagtatgat ttcttaaaaa gttcatatct
    19201 tttcttatta tctattactt ctgtgtgttt attatttaaa tcagataata tagatgacag
    19261 ttcttctata aattcttcat cattttcagt gttttttatt cgttttgtat attcatcctt
    19321 atttgctaac caatcaatat tattaagcct cttatttaca cctagatatg gatatcctgt
    19381 ctttacaaca ttatacacat aattaaagtc atccaccttt tcctcttcag acaactgatt
    19441 cttttgagaa taagtagaat tacctaataa gattataaat gctaaaataa ttactaaaaa
    19501 taatactttc tttttcatgt aattctcatc cactttcaga ttttatattt taatacattg
    19561 tatcattatt ttattatcac ctaaacagtt aaaagaaatt cttgaagata tattccacta
    19621 ttattctatt taaaaatttt tatttgtatt ttcaaaatta tttcatgaaa gttataaaat
    19681 aacaattata ttcaaatatc tattttactg ctatatcctg aaagtaaatt cttatcagtg
    19741 atatactaaa aaagatatgc taaaaaattt atccattata atgacttaaa tttagatact
    19801 ttactgattt ttatttatta gttgagaaag atattgataa ttaaaaatat tgataactat
    19861 agaaatgatt attttttata gaaattttaa ggatataaac taattatact taagtctata
    19921 tccttaattt atcatgatta tgagaatttt tacaaatact tatctttgtc cgttttagaa
    19981 taaaaacaag aacatatcct acttatttta ttcaaaaatc caagtttaat tactacacaa
    20041 ttacttatta gaaattgtat ctacttcata tatatatctg ttaagtatag cttctagcat
    20101 ttcttgtctt ccagacttat tctttataac tggcatttgc agtgcataag attctaactc
    20161 tttaaatcca accttacctt ctactatatc cttacctata ccttcactaa aacttgcata
    20221 tctttcttta gtaaagtttt caaatacctc atcttctaat aatttatgag ctattaataa
    20281 tccctttgca aatgtatcca ttcctgcaat ataagctagg aataagtctt ctacctcaaa
    20341 agatgctctt cttactttag agtcaaagtt aaatcctcct ggtgctattc caccttgctt
    20401 taacacttca tacatagcaa gtgttgcatc atatatattt gttggaaatt ggtctgtatc
    20461 ccaacctaat aataggtctc cttgatttgc atctatacta cctaaaacat tgtttattct
    20521 tgctatattt aattcatgtt ggaaagtatg tcctgcaagt gttgcatggt ttgcttctat
    20581 attcattttg aagtatttat ccagattata ctttcttaaa aatcctaaaa cagtagcagt
    20641 atcaaaatcg tattgatgtt tagtaggctc ttttggctta ggctctatta agaattggcc
    20701 agtgaatcct atttccttag cataatctac agccatttgt aagaatcttg caaagttatc
    20761 catttcaagt tctgtattag tgttaagtaa agtttcataa ccttctcttc caccccagaa
    20821 tacaaaattt tctcctccta agtacttagt tatttccata gcttttttaa cctgagctgc
    20881 tgcatatgca tatacatctg cattacatgt tgtagatgca ccatgaacaa atcttggatt
    20941 tccaaacata ttggcagttc cccataataa ctttatacct gtcttttcca tattaacttt
    21001 tatatactca actatttcat ctaatatctt atttgtctct tctaaatctc ttccttctgg
    21061 tgctatatct ctgtcatgaa aacagaaata ctcaattccc atcttagaca taaattcaaa
    21121 tcctgcatca actttaatct ttgctatctc aattgggtct gttatattat tccaaggtct
    21181 ctctactgtc cctactccaa atgggtcatt accctctcca cataaagtat gccaatagct
    21241 cattgcaaat cttaagtgtt ctttcatagt cttattacct accacttgtt caggattgta
    21301 gtatctaaat gctaattcat tgtcagattt tactccttca aattttatct gtcctattcc
    21361 tttaaatatt tcactcattt catgtcctcc taatatttta cacatataat atatgtagta
    21421 tgttttctta atttaaaact attttctatc tttatttttc tttttaagct aatttaatta
    21481 tttatttttg cattaactac ttcatctaat aacttaaagt catctttcaa agatttgtat
    21541 attcttttat acaatctatg atatatattg tatgtatcta cattttcact tattggttct
    21601 aatttagttg aagtttttat tatagcatca catgcttttt gtatattttc atatattcca
    21661 gctcctacta atgcaagaat tgcaactcca agtgctggtc cttcacttga atttatagta
    21721 tatacatctt gattaaacat atctgcttgc atctgtctcc atactttact ttttccacct
    21781 ccacctgagg ctcttacttc attaacgtct atattaagat tctctattat atccatacag
    21841 tctttaagac tatatgaaac accttccatt attgaccttg cgatttcttt tttagaatgg
    21901 attggagata atccaaagaa cacacctctt gcataagggt caagatgagg ggttctttct
    21961 cccatcatat atggtaagta aagcaatcca ttacatcctg ttggtacttg acttacttga
    22021 ttatttataa tctcatatac atcttcacct aaagaatttg aaacttccac ttcactttga
    22081 cagaaattat ctttaaacca ttttaatgac aatccagctc cttgagtaac tcccataaca
    22141 tgccatgtat ttggtattgc atgacagaaa gtatgaactc taccttcttt atcaattttt
    22201 ggctcttttg tataagcaaa tactacacct gatgtaccta tagtagatga aactactcca
    22261 tctttaacta ttccatttcc tatagctcct gctgcttggt ctccagctcc tcctaccact
    22321 attgttcctt ctttaagccc tgtcaaactt gctacatctc tagtcacata acctgtaatc
    22381 tcttgtgatt cataaacttt acctaaaaga tttttatcta tatttaactt ttctagtact
    22441 tcatcgctcc aatctctttt tcttatatcg agcaactgca taccacttgc atctgatacc
    22501 tctgtagcat attcacctgt caatttgtat cttatataat cttttggaag aagtatttta
    22561 ttagtatttt tatatatatc tggttcattg tttctaaccc ataatatctt tgatgctgta
    22621 aatcctgtaa gtgctggatt agcagttatt tcaatcagtc tttcttcacc tactaaatca
    22681 gttatctcct cacactcttt tactgttcgt tggtcacacc atattataga gtttctcaat
    22741 gttttacctt cattgtccag cataactaga ccatgcatct gtcctgataa tccaattcct
    22801 tttatacatt cactgcttat atttgaactt tgaactactt ctcttataga ttcgactaca
    22861 gctttccacc aatcatttgc atcctgctct gcccaacctg atttctcttg tataagagga
    22921 tattcatatg tacaactttt tatagtattt cctagtttat caaataaaac tgttttagta
    22981 cctgatgtac caatatccac acctaataca tactccaact tcatccctcc ttgaatgcca
    23041 cttcataact atcgttttcc tatcctaatt tttttatatt ttaggtatat tttacttgct
    23101 ttatttttgt tagtatatta aactaactta tgaactaata ataaaatatt taattatctt
    23161 tgtcaatgca aaataacctt ttatttttgt gtaaaaagta ttacaatatt attttaagga
    23221 ggaatatcaa attggttact gataaataca ctattcgaga aatgaatgaa agattggtac
    23281 ttgaacaaat tataaaaaat ggtcctattt ctagggcaag tatagcttct actataggat
    23341 tgaataaagc aactatatcc gctattacca aaaaacttat tgatgaatct ctagttcatg
    23401 aaataggaat tggaaatagt actcatagtg gcggaagaaa acccatatta ttagtattta
    23461 ataaatgtgc agggatttct ttaagtatgg acgttggata cgactatata ttttcttctc
    23521 tatcttactt agatggaact attataaatt ctaagaaatt aacagatata caagtaagta
    23581 aagacaatgt tatacaattg atagatgaaa ttataaatag ttataacata tccaaaatag
    23641 acactcctta taaggtaatt ggtcttactt tggctataca tgggattact tgtgaaaata
    23701 aagtattgtt tactccatat tacaatctta atgagattga cttatatagc atccttagca
    23761 aaaagtatga ctttcctatt catatagaaa atgaagcaaa cttaacagct ttagctgaga
    23821 atactttttc aactgtacat aactcactac ttagtctcag tattcacagt ggatttggtt
    23881 caggcattat aataaataat aaattatata gtggcagaaa tggtatgagt ggagaaattg
    23941 gtcacactat catcatgcct aatggtaaac tatgtccttg tggaaatcgt ggctgtttag
    24001 aacagtattg ttcagaaaaa aaggtatttg aacagctttc ttctttagaa aatattccta
    24061 aaatagattc tgatatagta aagcaactat attatgaaga taaccagaat gccaaaaaag
    24121 ttattcatga gttttgtagt tacttaacaa tagctataaa taatgctatt acaacttatg
    24181 ctcctgaaat aatatattta aatagccaaa taattagtga tatccctgag atattacaga
    24241 ttacaaaaga tatgttagtt agctctttta acaaaggaat aaatatagaa atttcatcat
    24301 tagggtctga ggcatctcta tatggtggct ctgccgtaaa tatcaagagc tttttgaata
    24361 tacaaaattt aactttaatt aatgaaatat aagggttatg actatataca gccataaccc
    24421 tttaaattat acattctaaa attagattta ttatatagtt tatgcaagat tagctatatt
    24481 ttttaaaaat tgtatatcaa ttaaatatta acataagtat ttcgttttaa atattaatat
    24541 aatcttccta ctcatcatca gataataaac ttgcattttg ttcaaccata gaaatattat
    24601 ctttacctat tttctctaat tttgctgcca attcctttgt atctaaaaaa gctgattcaa
    24661 taaccatacc taaatttagc cctgaaataa ttttaattcg actgtcatta ttggctacta
    24721 ataaagcagc attacatgga gttccaccta gtaaatctgc cattataatt atttctgaac
    24781 tttccttgta cacttctaaa gcttttttta atttttctat tgttccttct attccatcat
    24841 cttgtttcat atttacagta ggataatcta tagaatcccc tattatcatt tttgcagaat
    24901 ttaagacttc ttcagccata ttgccatgac tcatcaaaat aatactattc aatttaatac
    24961 acctctttaa atctataaga tgtctacaag ataagttttc ttatcatcag gcgtcattat
    25021 agttgtaatt tcaattcctt catctaaaag tcttttaaat gcttctttct cttcttcagt
    25081 aacactgata ttaggtctta gaactgttgt atcttttcta cttgacatat ttcctacatt
    25141 tatattatgt attttaaatc ctgcctctac aaatggaata aggtccacag gagacttaac
    25201 aattaataat acattttgtc cttcatactt tcctgcagta atatttttta tagcagtctc
    25261 ttctgtaatt atagatgacg ctatacctgc aggtgtagcc attcttaaaa cttgtttttg
    25321 aacagaattg ttagctactg catcatttac taccattaat cttgtaacac ccaatttatt
    25381 actccacata gttgcaactt gaccatggat taaacggtca tctatacgaa tatttttaat
    25441 acctttcatt tccatatcta atatcatcct ttccttattt taaaatacct aatgcatata
    25501 aaattattga aacaaccatt actcctaaaa ttaagtatat aggttttata ttctttttaa
    25561 gagcccaata aacaatcaca actagaattg caggaactaa cccaggcata atttggtcta
    25621 atatttcttg acctttcatt gtaaaatcac cttgttgaaa gttcaaagca acattagctt
    25681 ttacaactga aggtattaat gcacctacaa ccattaaacc taaaatagat gtactctcag
    25741 ttaaaatctt taaacttttt tcaattgaag atattagctt agtacctgat gtataaccag
    25801 cattaaatag tggtatactt aataatttca atgctacact cactacaatc cacagtaaga
    25861 ttccaaacgg acttccatct atcgccatat tagctgctat agctccaaaa attgtccacg
    25921 gaataacgac aaataaacta tctcctaaac ctgctactgg tcccatcaag cctgttttaa
    25981 ttgaagcaac agcctcttca gtctcttcac ttgataactc ctccattgct acattaatac
    26041 ccaaaatata aggggcaacc caaggattag tgttaaaaaa ttgtaaatga tttaatacag
    26101 cactttgtaa cttttctgga ttatctttgt atactacttt taaaaaaggt agtactgcat
    26161 aacagtatgc caaggcctgc atcttctcat aatttgaaga aacagaacta gtaaaaaacc
    26221 agcgccataa gacacttttt ttcacctttt catttgattt atttaatgat accttatcac
    26281 tcatcttcgt ccatacctcc tactacagta tattgttgtt cttgattttc caaattcttt
    26341 ttgtaaatta taagtgcaat tgctccacca attaaagcta cacctaataa tggtactttc
    26401 atatatactg ctaaaacaaa accaattact aagtaactaa aatactcctt agcaggaaga
    26461 tatctaagta ataatcctat ccctactgct ggaagtagtc ctccagctac tgttaaacca
    26521 cctgttaacc attcaggtgt atatttcaaa atggtattaa ttaaagaagg tccaaatatt
    26581 acagatagag caactggaat acctgttgta gctgctgtta atagtactcc taacatttgc
    26641 atataggtta tatttttata atttcctttt ttagctccat cttctgcttt atgaactagc
    26701 catataccaa tagtatttct aagaacatct aattgtacca tcaataaagc aactggaata
    26761 cctatagaga ttcctacaga tgcttcttgc ttagtcgtaa tagttataaa tgtggctact
    26821 atacttgctg tttgataatc aggtattgat gctccaccaa agttactaat acctaatgat
    26881 aatagctgta atgtacctcc aatatataat cctgtattta tatctcctaa gataagacca
    26941 gtaataaaac ctgctgttac tggttgatat gttccaaaca ttgttgaatt tttttcatag
    27001 tttataaaaa atccatatag tacgattaat aataattgcc ataattgtgg ctccattatg
    27061 atttcacctc tcccaaaata ttttttagtt ctttaaaatt ataactggaa tcttgcttta
    27121 ggaaaatagg aatttcttcc agtgggcatt caacttcata agttttccca ccttcataac
    27181 taactccact aaatatattt tcccatgtat gtccttttgg taaatatact tttctacttc
    27241 tatctttata attcattatt ggagctacta aaatttcatc tccaagcata tatgtattat
    27301 ccacctccca agttttatca tcttcaggaa attcataaaa taaagttctc ataactggtg
    27361 ttccatcttc atgtgcttct ttcatcagtt tttttaaata agtttttaag ctctctctaa
    27421 tttttatgaa ttttgtaaga attacttcaa cttcttcacc ataagaccat atttcattag
    27481 gagctccagt taccattgag cctccacctt tatttgataa tggtttacta tgaggcaatc
    27541 tatctccatg cattctcaaa attggtgaaa aagtagcata ttggaaccat cttaccataa
    27601 gttctctaaa ttctgggtct ttaggatttc ctccatggaa acctccaata tcagtagtcc
    27661 accatggtat acctgccagt cccatattta accctgtatt tacttgatta ttaaaagctt
    27721 caaaactaga atcaatgtca ccagaccata ctaaagctcc atacttttga gctcctgccc
    27781 aagctcctct tacaagagtt acaacagact ctatgtcatt atgtaaacca tcataaatca
    27841 tttttaaata atcaatagga taaatattac cacaacttaa aacatttcct tttttatatc
    27901 tatagttatc aaaatcatac acagaatagc caggttcagc tacatctaac caataatagt
    27961 caatcccaag ttgtttataa tttttatcta ttctatccca aacatattct cttgcatttt
    28021 catttgtcat atctacaaag actgtatttc cttgaatttg cattgtcatt ctaacaccac
    28081 gattaacatt gactaaatag ccattttcta aataatcatt gtagttttct gcatcacttt
    28141 gaactgtagg ccatacagat actattggtt ctactgaata ctcttctttc aattttttaa
    28201 ctaattcttt aggatttttc caataatcta agtcaaattt atattcacct tgcttaggcc
    28261 aatgaaaata gtcaattgca attgatgaaa gcttaattcc tcttttagaa tattccttca
    28321 caacatctaa aacttcttct gaagttctat atcttagttt actttgccat agtcctaaaa
    28381 ggttttcagg catcatagga acagttccag taacttgaga gtaattttta tttaactctt
    28441 ttgggctttc tccacaagta atccaataat ctataaaatt tgtagaaaac atcttccatt
    28501 ctgtcatatt tttagcaaaa gatacttttc caattcccgg attattccat aaaagtccat
    28561 aacctagact agaaatataa aaaggaactg atatttgaga atttctttgg gctaactcca
    28621 aaacagtatt ctttaaatct aaaaatttat gttgatattg tcccattcca aaaatctttt
    28681 cagaaggctc agattcaaat ctagtagtta cttcaaattc tccactatag cttggtcgat
    28741 attctcttga ttttaacttt agagtagagt taaagtcttt ggtaatctca attgtcccta
    28801 catcttctcc accatcatca tgttttacag ctcttaatcg aatatattct ttcaataata
    28861 tttcattttt atcattataa aaagtaattc tatctctatg gtctaagaca gcttttatct
    28921 ttccattttt aatgattgca cttccatctt catttttatt aattgtaata tcatctttat
    28981 ttaacttagg tttttcgtta agagcatagt tttcatcaac aaaattttgg tctacaaacg
    29041 acctaatacg taaagagttt tttccccatg cttctattcg caatagttca ttgtcaaatc
    29101 ttttttcaat atagtcttca gatattttta tcacggctac ctcctaaatc aatggtataa
    29161 tattctatct ttgtgtagct taagttaaat aatgtcgttt agtatcgtta tttataaatt
    29221 aggttttata tataattcat ttgtacatta aactaactta ccaataatat actttatttg
    29281 actttgtttg tcaatcgctt tccggatttt agtaaaattt tctaatatat tgctggaaag
    29341 tcgttcttaa tatcttttat ttatactttt aatatctgtg atgataccta tatacatcta
    29401 gtttttttaa aacacataca ggaataaagt ttgcctatta tgtagataat atcggacttg
    29461 aaaatatgta tagtgacctt ataaatacag ataataatat attagatatt gttcaaaaca
    29521 atggatttta taattataaa taatttatga aattatttaa taaaatatat aattgtatac
    29581 caagtcaaaa acgacaacaa ataaataaaa ctaaaaaaga tgttgtctca aaatatagat
    29641 aaatttattt ttcattgcta aaatgattaa aatatatttg atttaatata cttgatttaa
    29701 tataaaaaaa gagtgtctca taaactaaaa agttcacttt tagacactcc tttatatata
    29761 ttatctactt cttaattcat ttattacctc tctacatttt ctttcaacta gtggatacat
    29821 ctttataagt actaattact ccacttaaaa atggttcctg tactgtccat cagtcatatt
    29881 tttctgccaa atataatggt gtttcataat gagacaataa atatttatat ctctacttat
    29941 cttctctaag ctcgtcaaat ccaactatag gtatatttaa attatattct ttaagtagct
    30001 tttctagctc ttctataaaa atacctttcc catccaccat ttgtatatca tttattttat
    30061 ccatcaattc attgcaacca cttaactcac caaaccaacg tttagaacta catgtcttat
    30121 tataaccaca acttggactg ccatctattg caatgatact ttttatatca taatcatttt
    30181 ttaaatattc atcaaactgc cttacatgat gtattaacat ttgcctacat tgttctctaa
    30241 aaaacaaatt gtcaaactgt tcttttacat gtccccacct atttattcca tacataatca
    30301 tttctgggca tggtagttga attattccat accctttatt tattaaatct atcgctatat
    30361 tttgtgctcc cttaaattct gacaatccct ccactttaga attacaattt aatatacaat
    30421 gacatactac tacaatctct ctatttcttt tcataataat ctccttagtt attcttaatt
    30481 ttaatgatac tacatatttt gataa
//
LOCUS       DAENCV010000041        29670 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAENCV010000041 DAENCV010000000
VERSION     DAENCV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.041"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(60..2726)
                     /locus_tag="KQD82_001826"
     CDS             complement(60..2726)
                     /locus_tag="KQD82_001826"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG4380300.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2802..3064)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3365..4192)
                     /locus_tag="KQD82_001827"
     CDS             complement(3365..4192)
                     /locus_tag="KQD82_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4380301.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4240..5676)
                     /locus_tag="KQD82_001828"
     CDS             complement(4240..5676)
                     /locus_tag="KQD82_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG4380302.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5763..6632)
                     /locus_tag="KQD82_001829"
     CDS             complement(5763..6632)
                     /locus_tag="KQD82_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG4380303.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6790..7443)
                     /gene="phoU"
                     /locus_tag="KQD82_001830"
     CDS             complement(6790..7443)
                     /gene="phoU"
                     /locus_tag="KQD82_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG4380304.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7471..8235)
                     /gene="pstB"
                     /locus_tag="KQD82_001831"
     CDS             complement(7471..8235)
                     /gene="pstB"
                     /locus_tag="KQD82_001831"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG4380305.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8254..9078)
                     /gene="pstA"
                     /locus_tag="KQD82_001832"
     CDS             complement(8254..9078)
                     /gene="pstA"
                     /locus_tag="KQD82_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG4380306.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9080..9997)
                     /gene="pstC"
                     /locus_tag="KQD82_001833"
     CDS             complement(9080..9997)
                     /gene="pstC"
                     /locus_tag="KQD82_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG4380307.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10378..10824)
                     /locus_tag="KQD82_001834"
     CDS             complement(10378..10824)
                     /locus_tag="KQD82_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380308.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            complement(11341..12021)
                     /locus_tag="KQD82_001835"
     CDS             complement(11341..12021)
                     /locus_tag="KQD82_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380309.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12071..14086)
                     /locus_tag="KQD82_001836"
     CDS             complement(12071..14086)
                     /locus_tag="KQD82_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG4380310.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILFPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(14158..14835)
                     /locus_tag="KQD82_001837"
     CDS             complement(14158..14835)
                     /locus_tag="KQD82_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380311.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15415..15498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15731..16564)
                     /locus_tag="KQD82_001838"
     CDS             complement(15731..16564)
                     /locus_tag="KQD82_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4380312.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNSGDSGDSSKVSVSGSTSI
                     GPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSAGLK
                     ETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREEGSG
                     TRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSLKIN
                     DVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFIKVN
                     Q"
     gene            complement(16816..18600)
                     /gene="pepF"
                     /locus_tag="KQD82_001839"
     CDS             complement(16816..18600)
                     /gene="pepF"
                     /locus_tag="KQD82_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG4380313.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KIIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18666..19331)
                     /locus_tag="KQD82_001840"
     CDS             complement(18666..19331)
                     /locus_tag="KQD82_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG4380314.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19482..19643)
                     /locus_tag="KQD82_001841"
     CDS             complement(19482..19643)
                     /locus_tag="KQD82_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG4380315.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19721..20230)
                     /locus_tag="KQD82_001842"
     CDS             complement(19721..20230)
                     /locus_tag="KQD82_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG4380316.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20436..20654
                     /locus_tag="KQD82_001843"
     CDS             20436..20654
                     /locus_tag="KQD82_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG4380317.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20672..22618
                     /gene="feoB"
                     /locus_tag="KQD82_001844"
     CDS             20672..22618
                     /gene="feoB"
                     /locus_tag="KQD82_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG4380318.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(22797..23852)
                     /locus_tag="KQD82_001845"
     CDS             complement(22797..23852)
                     /locus_tag="KQD82_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG4380319.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(23900..24700)
                     /locus_tag="KQD82_001846"
     CDS             complement(23900..24700)
                     /locus_tag="KQD82_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG4380320.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24693..25484)
                     /locus_tag="KQD82_001847"
     CDS             complement(24693..25484)
                     /locus_tag="KQD82_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380321.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(25524..25946)
                     /locus_tag="KQD82_001848"
     CDS             complement(25524..25946)
                     /locus_tag="KQD82_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG4380322.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(25962..26441)
                     /locus_tag="KQD82_001849"
     CDS             complement(25962..26441)
                     /locus_tag="KQD82_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380323.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(26573..29329)
                     /locus_tag="KQD82_001850"
     CDS             complement(26573..29329)
                     /locus_tag="KQD82_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380324.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9534 a         5235 c         3230 g        11671 t
ORIGIN      
        1 aaaaacatac aaaaagtata gaatatatac tttttgtatg tttttactaa ttcttatatt
       61 tattttattt tagattctaa atttgttaat ctttcctcaa cagatttaat catctcttca
      121 aatttttctt tttttgcttt ttcttcttca attaaacttt caggagcttt tgctaagaaa
      181 ccttgatttg caagctttcc attaactctc tttatttcgc cttctaactt tttcttttct
      241 ttagataatc tctcaatttc tttttcaaaa tcaactaatt cattaagagg tataaatatt
      301 tcaacaccat caataactac tccaactgca tcttctggaa cattagattt atcttttgct
      361 atctctacat ttgaagcaga tgctaaagtt ataaaatagt ctttaccaag ctcaatagct
      421 tctatttttt cttcacttgg gattattatc aatttagctt tcttagaagg tggtacattc
      481 atttctgctc tcacatttct tatacttctt ataccttcca tcaataagtt catcatatct
      541 tcttcttcag ccatgttatc ttcttcatta tattttggcc attcagatac tattatacat
      601 ccttcaacag taggaagatg agtatatatt tcctctgtta taaatggcat gtatggatgt
      661 aataatttta atattttttc aagaacataa gttaatgtat ataatgctgc agatttagca
      721 tttgcatctt ctccatataa tctaggttta accatttcaa tataccagtc acagtattct
      781 gaccaagtaa aatcgtatat cttttgaagt gctattccta aatcaaactt atccatgtta
      841 ttagttgctt cttttacaat attatttgct cttgatatta tccatttatc agctagagtt
      901 aagtcctctt taacactttc tctagtcatg tttttaatta tatcttcatc aatattcata
      961 aatacaaatc ttgatgcatt ccacaactta tttgcaaagt ttctagcaaa ttctactctt
     1021 tccatataga atctcatatc atttcctggg gaatttccag ttgttaatgt aaatcttagg
     1081 gcatcagctc catattgctc gattatctcc aatgggtcga taccatttcc taaagattta
     1141 ctcatttttc taccttgaga atctcttact aatccatgaa ccaaaacatg gtcaaatggc
     1201 ttttcattca tacagaacat acctgcaaat gccattctaa ctacccagaa gaatattata
     1261 tcgtatcctg taacaagtac acttgttgga taataataat ctaaagcttc agtcttattt
     1321 ggccatccta gagtagagaa aggccataat gcagaactga accaagtatc taaagcatct
     1381 tcatcttgtt taaaatgagt gcttccacat ttaggacact tatctggcat ttttctagca
     1441 actactattt ctccacactc ttgacaataa taagctggta tttgatgacc ccaccataat
     1501 tgtcttgata tacaccaatc tcttatattt tctagccatt gtaaataagt tttatcaaat
     1561 ttatctggaa caaattctaa ttctcctttt ttaagaatat ctatagccgg ttttgcaagt
     1621 tcttccatct taacaaacca ttgctctgat aatctaggct caactactgt atggcatcta
     1681 taacaagttc ctacattatg attatgctct tttatcttaa ttaggtaacc ttgttctttt
     1741 aagtctgcta cgatagcttt tctacactca aatctatcca taccttcata ttttccacaa
     1801 acttcattca tagttccatc ttcattcata gtatttaact gctcaagatt atgtcttaat
     1861 cctacttcaa aatcattagg gtcatgtgct ggagtcatct taactgcacc agttccaaac
     1921 tctaaatcaa cataactatc tgcaactatt ggaagttctc ttcctactaa tggaagtatt
     1981 gctgtttttc ctattaaatg tttatatcta tcatcttctg gatttactgc tataccagta
     2041 tctcctatca ttgtctcagg tctagttgta gctatttcta aaaagtcttc actatctttt
     2101 agtgggtact ttatatgata aaagtttccg tcgtgttctt catgttcaac ttctgcatct
     2161 gataaagtag ttttgcaatc tgggcaccag tttattattc tatttcctct gtatatgtga
     2221 cctttttcat ataggcttac aaaaaattct ataactgctt cattacaccc ttcatccata
     2281 gtaaatcttt ctttatccca atcacaagaa tcacctagtt gtttaagctg attagatatt
     2341 tttcctccaa attcatcttt ccattcccag gctctcttta aaaattcttc tctacctatt
     2401 tcatactttg tcttgccttc ttgttttttt attctttcaa caactttaac ttctgttgct
     2461 atagaagcat ggtcagttcc tggaagccaa aaagcttcat atccatccat acgtttccat
     2521 ctgattaata tatcttgaag tgtatggtca agagcgtgtc ccatatgcaa ttgccctgtt
     2581 atatttggtg gtggcatcat tattgtaaat ggttttttat ctggatttgg tttagattta
     2641 aaataccctt catccatcca ttttttatat agcctagcct caaaatcttt tgggttataa
     2701 gtttttgata aattcgtatt ttccattgtc ctgcctcctg tattttataa ataaattttt
     2761 taaatatctt tagaaataaa aaagagctct tcatcctctt taaaaaggac gaaaagctcg
     2821 cgttaccacc ttaattccaa agcttaaaat acattaacat ataattaaat tattggttaa
     2881 tgttatttta tcaactttga ctctcaaaga atttaacggt tctaaccgtc taaacttact
     2941 tatagttcag tttagaaact ccaaagctac attcagtaaa tcttatactt agaaaatctt
     3001 acagccagtg aattttcctc tcttttagta gtatttactt actcctcttc ttctcagtct
     3061 ttaatattac atattattat atacaaatat tattctcttt gtcaattatt tttattttat
     3121 tttttctgaa ttttcttaat tatgtctatt ctcttttata tagttgttat attttaaaat
     3181 atgtttttac agttattctg tcttataatg tcaataattg caagattatt ttattttaat
     3241 tttaatgctt tctcattaat tttatattat attatcctta cagtatacct cttattcgta
     3301 ttttttatat tatatgtttt attttataaa taatttactt tatcttttac tatatttaaa
     3361 tttattataa gttttcttgc cagaaatttt taggtgttat attttctgta attggcaata
     3421 ttgtatatcc tctctctttt aagattctta taatattatt taaagcattt aaactttgct
     3481 ctttctcatg aattaatact actaattcat ctctttttct tccataatat aatatattac
     3541 ttactatctg acttgtagaa gacttccaat cctgtgtatc caaattccag tcccaaatta
     3601 aataatcatt tgccataagt ttattatatg aaccttctgg catatatggt ttacttccgt
     3661 atggtaatct tattaatttt gaactctgac cagttatttc ttgtagagta ttattgcatg
     3721 tattaaattc cccaactgtt acttctgggc ttttatataa cttatgtata tcatgactta
     3781 cactatgaaa acctattgag ttttcttctt ccaagactct ccttacttct tccttgtggt
     3841 ttttcatatt ctgattaagc atgaaaaatg tcgctttgac atcattttct tttaaaatat
     3901 caagtagtgc atttgtatat tttgatggac catcatctat tgttatgtat gctacttttt
     3961 cagaagtagc ccctggacct tttgtttctt cttgagatgc tttgtatatg atttctccta
     4021 aagtattact ttgagaaagc agttccagtg tattttcacc ttttgaactt atattttcaa
     4081 ctggaacatt attaattttt tcctccaata atgttccagt taaaattaaa acagctataa
     4141 taaatatcct tataaataca tgaaaaacag ccttattatc tttaattttc atcccccata
     4201 tctccctttt ataaaagcat aagtttaaga cttaaaaagt tattttttat atttattctc
     4261 tttcttatta ctttttatat ctctactaaa tattttttta accttttctt cttctttttc
     4321 tcctaaagat tctactcttt tgattcccaa agaagttaaa aattgtaata catttaaatt
     4381 atcaaatgga gtttccatat tcgtactttc actatattca tcaacattct tagtaccttc
     4441 ttccacactt aaaatacgct tcggacctcc tacacagcct cctacacacc ccattccttc
     4501 tataaatgta gcttctattt ctttatttag aactttcttt agaccgtcca tgcactcttt
     4561 tgttccttca aatgcctttt cttcaaattt tatatttttg tcaattcttc taactgataa
     4621 cttaactgct ttacttacac ctcctgttct agcataaact ctacctgaaa gggacgcctc
     4681 tactctatta ctctcatcca ttattgctgg atttaactct aatgcatcaa atatttcttg
     4741 caactcttta aatgtaagca caaaatcaac tgcatctttt atatcttcta acattgcctc
     4801 tttctttttt gccatacaag gacctatgaa tacaacttta gcatctggat ttaaaatttt
     4861 tacagctctc ccacaagcaa tcataggaga aacggatggt gatacatttt ccaatatctg
     4921 aggaaaacta ttttgtatca aactaaccca aataggacag caacaactag ttataaagca
     4981 tccttcttcg tcctttttca tatgctcata ataatcatat gcttcttttg cagtaagcat
     5041 atctgctgcc aaagcaactt ctatcatatc ttcaaatcct atctttttta atgcagttct
     5101 taatttacca gggcttacat ctttcccaaa ctgtcctaca aaagctggtg caactattgc
     5161 atatacaggg ctctttatat cttttaacaa ttttatcatt ggtataaatt ctattttatc
     5221 tgaaattgca cccaacttac atcttgatac acaatcacca caagcactac atacatcttc
     5281 ctcattacaa ttaatatgct tacttcttat cttacatgac tcatcttcct tattattttc
     5341 atcacaatat ttgcaagcat ctgatattac agatattatt ggtcttttga cttcatctaa
     5401 atttacagct tcatgtagtg actcttttaa atttttatct gtatcttctg tcacatccaa
     5461 tcccatagtt accctaatta aatttaccat agtatcttta tcaaattttc cactaaaata
     5521 attatttaaa ttatccttta aatcttcatt taaaatttta ttaggtaagt tttcaatatc
     5581 ctctatatca tcattccaca ctgcttttac aagttcgcca aaaagcttca ttttatatat
     5641 ttctagtatt ttagaattgt catttctagt attcataaaa aatagtacct ccataaatta
     5701 aacttatata tgtttaatat ttacaaagat actatttttt atttattatt taactactat
     5761 tattaatatg atttatttag ctttctcaac aactctattt caatcattat tttcattaga
     5821 tgatagttga atacagggct tttttcttca tctatattcc agtctagctt atccacatta
     5881 tatgttttct taaggtattt atatgtatgc tctttcaaat aaagtttatt ttcaagtaac
     5941 tctatagact gcatatgaaa atgtatttct ctacatatcc ttaaggcatt ctcaattttt
     6001 tctatattag tttcactcaa actataatcc aactcatcaa taagttttga atatatttct
     6061 tctaattttt ctacttcttc ttcaaatttc tctacattta aattttcttt tctaagaatt
     6121 ttatcttcaa taagcaacat tgaaagttct tctatctttt taaactcatc tataacacta
     6181 tttagataat ttggtctagc aacataataa ttaacaagta ctccaaacaa aacacccaca
     6241 gatgtatcta acaccctatg taaagaataa tacacagggt cactatttcc tacacctaga
     6301 tgtatagcca gaaaggttac acaggcaact actactgact ttattttaaa cagattgcat
     6361 aaatatattg agcacattac tcctagacca cataaaaatg ggtctccagc tttaatgatt
     6421 gcaaatatga atcctattat accacctaaa atagtacctt ccactctatt gattcccgtc
     6481 tttaaagaac cttttactgt atcttgaaca gaaattatac atgctaccag agaataaaac
     6541 atattctcta ctaaatagtc accaagaatc atacataaag tgacactaat tcctgttttt
     6601 atagtcctca tacccatctt ttgaagtttc aaagttccac ctcctaaaaa gcaccttata
     6661 taaaatacgc atcctcatat ttaatatgtt cctagtatat cacaattttt taaatttacc
     6721 cattaatcat atttttttat caattctttc ttaatttaga caataaaaaa actgctttaa
     6781 agcagttttt tatcctatct caaccatttt accctcaatt gcaaaaatta tcttctcaca
     6841 tacatttgtt atatggtctc caattctttc aaggtatctt cctataagta gaagttttac
     6901 accttggtct atggtatttg agttttcatg cattattttt aaacaatcaa tttgtacttg
     6961 ttcatacaat ccatctacaa tatcatcttt agttccaaca tcataagcta tacttgcatc
     7021 aaataagatt aaagcttctc tagtctgaga catcatagac ttacattctt ttgccatctt
     7081 aggaatatct attagctctt ttatataagc ctctccacct atcttcaaca tttctctagc
     7141 tatatttgct gcataatctc ctattctttc cagctctata gatatattac ctaatgaata
     7201 aataaatctt aagtcccttg ccattggttg ctttaatact attagctcta tactcttatc
     7261 tcttatttct tctcttaatt tatttatttc ttcatcatct tctactactt ttcttgcacc
     7321 ctctaaatcc ttctctatca tacaagtcac agacttttct attgcttctt cacatttttc
     7381 catcataagc actgtttgtt cttttaaagt atcaatactt atatctaaac tattattgac
     7441 cattgtctaa ctcctttata gttatatatt ttaaccaaat ctacctgtta tatagtcttc
     7501 tgttctttta tctacaggag tggtaaacat agtttttgta tcgctaaatt ctataacctc
     7561 tccatttaag aaaaaagctg tttcatctga tatacgagca gcttgttgca tattatgtgt
     7621 aactatgact attgtgtagt ctttttttaa atcctctata agttcctcta cttttaatgt
     7681 tgaaattggg tctaaagcag atgtaggttc atccataaga ataacttctg gtctcatagc
     7741 aattgctctt gctatacata tacgctgttg ttgaccacca gaaagtccta gtgcagatga
     7801 tttaagcctg tctttaacct catcccaaat agctgcacct tttaagcttt cttctacaat
     7861 tttatctaat tgcttcttat ctcttagtcc atgtgtacga ggaccatatg ccacattatc
     7921 atatatactc ataggaaatg gatttggctt ttgaaatacc attcctactt tagtacgaag
     7981 atttataaca tcatctgagg tatatatgtc ctctccatca acagaaatgt ttcctttaat
     8041 tgttacatct tcgattaagt cattcattct atttaacgtt cttataaaag ttgattttcc
     8101 gcatccagat ggacctatta aagcagtaac cttattctct tttatatcca tatttatttt
     8161 ctttaatgct tgcttatcac cataaaataa atccaaatct tttacactca tttttatctt
     8221 atcgattaat tccattattc acacctctta aatttaataa ttagctttat ttaatttctt
     8281 agcaacaaat cttgctagca tgtttagtgc taatacaatt actattaaaa caattcctat
     8341 actggctgct gttgctatat ctccactctc tttagtcagt aagtatgagt gtacagttaa
     8401 tgttcttgca ctatcaaata ttccacctgg catcttggct acagtacctg ctgtaagtaa
     8461 tattgcagct gattctccaa ttatacgacc aactgataag attactccag ataaaattcc
     8521 tggtattgca cttggaagta ctaccttata taaagtttga aattttgtag ctcctaatgc
     8581 caatgatgat tctctataag cttgaggaac tgtttttaaa gcttcttctg ttgtacgaat
     8641 tattactggt aatattatta ttgcaactgt aagtgaacct gcaactatgg aatatcccat
     8701 ttttaatgta actacaaaga aaattcctcc aaataatcca tatactatag atggtatccc
     8761 tgataaactt tcagttgcaa atcttatagc tgtaactgct tttccttttt tagcatattc
     8821 ttgtaaatat atagcagcca atattcctat aggagttgct acaactatag aaaccactac
     8881 tgtatataat gttgtaatta tcattggaag tataccacca tcacctgatg ctgaataatt
     8941 gctaaataag aaattaaaat tcatgttgcc aattcccttt ataaatataa atcccactat
     9001 aactactaat gatactattg taaatagtgc tgatagatat actaatgact tcaatagatt
     9061 ctctttgaac tttctcatct taatttaccg ccttatttga aagtttattt aaaactaagt
     9121 ttaaaattaa tataaatgta aacaatacta ctccagttgc aaatagcatt tcttgatgtg
     9181 taccaaatgc gtatcccatt tcaagtgcta tattagttgt aagtggtctt acactatctg
     9241 ttattgcagt tggcataact ggtgagttgc ctgcaacaag tattactgcc attgtttcac
     9301 ctaaagctct tcctactcct aacactactg cggctaatac tccagatttt gcagcaggta
     9361 atacaacttt gaatattgtt tctatttttg atgcaccaag tgctaatgaa ccttctttgt
     9421 atgcttttgg tactgctctt attgctgttt ctgatacaga tattattgtt ggtaacatca
     9481 ttactgctaa tactattact attgctaaca aactttgacc ttttggaaga ttaaatatac
     9541 tttgtatagt tggaactatc actgcaagac caaatacacc atataatact gaaggtatac
     9601 ctgctaaaag ttcaactgct ggtgacatta tttttgctaa tcttttaggt gctacttctg
     9661 ctataaatac agaggttaat ataccaacag gtactcctat cacaagtgct ccaagtgttg
     9721 caaatattga tgctactatc atagatgata ttccaaattt attggcagaa ggaacccaat
     9781 ctgcaccagt taaaaagtct ataaatgaat atccttcaaa tataaatggt cttaatcctt
     9841 tataaaatac gaatcctatt atcaaaagta aacttgcaac tgctactaaa gcactaagta
     9901 gaaaaatatt ttttgctact ttttcaatta cgtattttgt cttattgcca ttattgtcat
     9961 ttttttctaa acctactaat ctagatttta ctatcataat accctcccat taaaatatac
    10021 ttatcgattt tcgttctgta ttcaaaattt aattttttat aattcgtctt catcaaattt
    10081 cctatacttt tattctactt tttttaagtt aaatctatat taggattaag ttaagcaagt
    10141 gtaaaattat atttactttt taacattaat tttactttta aaactatatt tgacatttac
    10201 tttacaatat actgttgttt ctctatatta aaatagcaga tatagataat aatacagttt
    10261 atgtataatt tattacctat atctgctatt aagatttgtt aactaaattt aacttttata
    10321 ctacatatat aattattgta tcctctagtt tatatcacta ttatttccat caaattgtta
    10381 cgatacttga tgtttgcaat aggtacgaat agccaaaaat ataaaaatag gtatttctat
    10441 cactgagaaa aacagtataa tgtgtggtgt ccaaaaactc atacctccta agtgtaagaa
    10501 attaaatgat gtcatttgat ataataaact aatcttaaaa ccaattcccc ctggtggtaa
    10561 taggtatcca atccaactaa cacctattcc cataggaatt aagcaaatag aaaaagcaat
    10621 cagcatagaa gtaagagaat ccttacattt tgcagataaa aataatgtta aactaaccat
    10681 tgcaagtatt gaaattagac caccaatagc tataagaatc tgtgcttgta gtaaatttat
    10741 atttactaga ctaattacag aaaacaacat ttgaaatgag gttttcaaac attcagtacc
    10801 aaaagctaaa tcagaaattg ccaaatggat agaaatacat ataacaaaca ttattataaa
    10861 tatagaaaat agagctaata ttttagtaaa cgctagacga atatgtccat gtttcgtaca
    10921 acgaaggatg ctatctgatt gtgtctgata ctcaccagaa aaagtaggtg cagcaattgc
    10981 aacacatata attaataata taaaaatata aaatattata taatcgaaag catcttctgg
    11041 atttattaag aaattcttaa tcttattatt taactatttt attatttaat atatattaaa
    11101 ggtaaaaaat aatatataga tttagatata caatttattt atcttttcaa atataatcta
    11161 taaaataata gttctgttac gcattttttt atataataaa actgactgta atttaattgg
    11221 caaagtctag aagaataggc tttatcaatt aaattacagt cagctatttt ataataattt
    11281 agtttagata tcgttagtat ttcacattta taacaattat taatgatatt aatatattta
    11341 ttattcttta ttaaaataat atcctgctcc tcttattgtt ataattttct gaggattttt
    11401 ataatctttc tctattttct ttcttaatcc attcactgca actaccacac tatagttatc
    11461 ctctaagtat tcattcatcc atacatgttc atatatctct ttagtagtca aagtcttttc
    11521 aggattctct ataaaaaagt ttaacaactt attttctgtt ggagtcaatt ttattatttt
    11581 atcttctaac tttattactc cactagttct attatattcc tctaattttg tattattttc
    11641 attattattt acatatgagt tcattcttct taaaatagct tttacttttg cgtataatat
    11701 ttctaaattt agaggcttta ttaagtaatc gtctccacca gaatttaaag catttaaaat
    11761 gctttggtct tcatttatat aagtcatata aacaattgga caagtagtta catttctaat
    11821 ttttttacaa agagtccatc catcaccatc acttagaatt atttctaaaa atattaaatc
    11881 atatttattg ctaaatattt ttccaatagc ttcttcacaa ttatatgcta aatctatact
    11941 tattcctttt tcctccagaa atccttttag cttctggcaa ttatccttgt ctccatctat
    12001 tatcaatatc ttagtttgca ttataatcac tcccaaatat cattattctt ttatattaaa
    12061 taagctatta ttataaaaaa catagactta attttattgt aaaaacagtt ccttttccta
    12121 gttctgaatc tacaaagatt tctccaccat gctctcttat aatggatttg acaatatata
    12181 gccctaaccc ttgccctttt ggctcattat tgtctctttc tgtaaagaat ctgtcaaata
    12241 cctttggtat attttctgga ggtatacctt ttccattatc agaaatttta ataattgcaa
    12301 atccatcttc tttatccatt gataaaatta tctttccatc aaatggagta aaggtcaaag
    12361 cattataaat taaattctca aatgctcttg taagcatctc tttatttatc tttgcatata
    12421 tttttttctc caatacgtaa gcttcaaagt ttacaccaca tgcttgtgta tctggcaaat
    12481 tattttcata tatctcttta ataaaattat tcagacatat tttttcacta ataccaatgc
    12541 ttttatcttg tgatgtaaat tcttgcaaac tacgtactct attttgtact tcatttgact
    12601 tttgatttat agcataaaga taggttcttg tctcttcatc tacatgaata ttaccaacct
    12661 ttatcaaatc tataaatgct tgtatagccg aaacaggtgc ctttaaatca tgtgctacat
    12721 ctgccaagaa tcttttacgt tcatttaaca atgttgtaag ttcattagtt ctcacttcaa
    12781 cttcatgttc aagattcctt gtaagttttt cattctcttt tattattatt ctattcctgt
    12841 ttatcatcat aacagaaaat aaaataacaa ttacaaaccc acaatattct gtttgccacc
    12901 ctgtaaatat aggctcaaat ctattactcg taactgcatc aaataatatg ctaattccaa
    12961 aaacagtgtt tactgaaact aaaatataag aacttatatg ctcagagcat gtctttttat
    13021 atattgaaac cagcgaagac attaatatat atataaatac aatatattta tatatgtcta
    13081 ttaacttccc atatatgttg acaaagtaca tatcatatgg aaaaagaata attggtatca
    13141 ctatagatat gatactcatt gtaattccta atggtaaaat taaaaatctg tacactttat
    13201 tttcttctat tttggatata cttccattta tacaaataat acataaaact accataaaat
    13261 atgacaaatc ttcaaccgca tatgtaaagc ttaccaaagg cagtcctaac aaatgtacaa
    13321 agggatagga tacatggatg gcaaagaaaa cacacataca tccaaaatat aagtaaattt
    13381 tacttttgtt tgaaagaaac catacagaca atgaaaatat agctattgca aaagttgtga
    13441 agcaaattat actataaaaa agtaatcgat aaaacatcat attacttatg tcctttgttt
    13501 ctcctaatgc aggcggataa tacaaaccac tataataatg tgaaaaattc gatacattga
    13561 ttataatttc tgtatttttc tttgcaataa aacttactac tgaattttta attttgggtc
    13621 tatatttttc tgttcctaca tctcctaatt tagaaaccat ttctccattt atccatactt
    13681 cacttgcgga aaaaatctca ggaagctcta aacttagtag cttatccttg ccttcatttt
    13741 ttattctcat acgataagta gcattcccat atggtgactg agtccccttt ctcatagaaa
    13801 aatctggata ttgacctata aaaacatatt ctggttttat atcttgctct gtaaaatcat
    13861 tcggtttaaa tattttatga ttataaaact cccagtcatc tatcaaaaag acaagtttac
    13921 ctttagtgaa agtatcatta tctatagtta aaattccatt acttgctcta atagacttat
    13981 atgtatattt attatcatat ttatacagaa gatttaatcc tgttattgcc aacagagaga
    14041 ctatcaatac aatgagagtt ccttttagta tattatactt tttcattgta tcaaccacat
    14101 ctctttcata gtagtattaa ataaaacaag aactatttat taattacagt atctcactta
    14161 tggtacaaac ttatatcctt ttccccatac ggtttgaatg tattgatgct ccctgcttaa
    14221 agaattaatt ttttttctca tctgtgcaat atgaacctgc actgtatgtt gatctccaat
    14281 atcaggcatt ttccaaactt ggttatatat ctctatcttc gaaaatactt gttctttgtt
    14341 ttcagctaga aacaataata tttcaaattc catagtagtt aaatctatac tttctccatc
    14401 aaaagttact tttcttgaat ttacatttat tactaacgct gaaaacttta acacattagg
    14461 aactgatgta atatttttgt actgcctcat acgagcatat attctaagtt caagttcttt
    14521 taaactatag ggttttgtta tataatcatc ccccccagct aaaaatcccc gaactttttc
    14581 ttcttcatgc tcataattag ataaaaatat tataggtatg ttaacctttt cttttatatc
    14641 attgcacatt ttatatccat ctatatctgg caatgaaata tccaaaatta tacagtctaa
    14701 aactatactc tctactatat ccatagcatc tttagcagtc tctgatgtat atacattaaa
    14761 acctatatta gaaaaataaa tttgatttat gtttaaaata tcaacatcat catctacaac
    14821 cagtaaatta tacattacac cactccattc aaaggtattt taattataat tccaagtttt
    14881 atagttatta caaattataa tgttttattt tcatatataa ctggtattat tattttataa
    14941 tatctttaca aactagacta taatacttaa gtatttttga ataattcttg aatattgtat
    15001 taaacattaa gattcattat taaatactaa ttaaagtctc cgattgttgg agaatggagc
    15061 ttaaagtcat tttcacgtat aaatttatta tttttctaga aacttgtcta ttggctagtt
    15121 tgagtattga tttatatgat ttatatggtt ataatataaa ataaattgta ttattttggt
    15181 aaatatattg ttacaaaagg tttatattct gcatctcaat gtttataaaa actttatttt
    15241 cttattataa tactattatt cttttaaaaa aatttccctt ttccaacaaa tggctacttt
    15301 aaaagccaaa attttaccta tcaataaaaa tcttaacgac taatactcct attttcaaat
    15361 tttgcaacat atttgttaat tttaaaacaa aaaaagacta tgcaatataa aaattatatt
    15421 catagccggt tgcaccacta actccatata tctctagatg cccaataata agatatactt
    15481 ctcagtttct aaaatatttt tattaaaaat cttttatata ttttcataaa tttctttttt
    15541 attctaacat tgtaatatta ttttgtcaat atttccatat tcttagagat aagatactaa
    15601 aatacctatt ttatctaaag atatttatat ttctacattc aataatagac aataccctgc
    15661 aagttaaagc atcattttta tgagcttatc tcacagggta ttatatgttt actttatatt
    15721 ataactcttg ttattgatta actttaataa atcctttttc tgaaacaagt gcttgtcctt
    15781 catcacttaa tatataatct attaaagctt tacctttttc tgacaaacca tcttctttat
    15841 taactaatat aaatggtctt gatattggat atttattagc tattacattt gcatcagttg
    15901 cttcaacatc atttattttt aaactattta ctttctcatc aacatatcca aatgatatat
    15961 atcctatagc attttcattt ccttcaactg tagtttttat atttcctgaa ccatcactta
    16021 tttgtgcatc tttagttagt tcttctgaat caaaccctac aatttcttgg aatccatctc
    16081 tagttcctga accttcttct cttgatacta ttactattgg tgcatcattt cctcctactt
    16141 ctttccagtt agtaatcttt cctgtatata tacctttaac ttggtccatt gtcagatttt
    16201 ttactgagtt tgcagtatta gttatcaatg ctataccatc tattgctatt ttagtttcct
    16261 ttaatccagc acttttttct tcatctttta aatctcttga actcattcct atttcagatg
    16321 ttccttctat agcatttttt atacctccag aagaaccaat ctgttgtact tctacactaa
    16381 caccttgatt tttttcttga aacttttctc cgatagtttc cataagagga cctattgaag
    16441 ttgaacctga aacagaaact ttgcttgaat ctcctgaatc tcctgagtta gagccacatc
    16501 ctactgctaa agctccaact aacattgtac tagctaaaag aacgcctatt ttctttttaa
    16561 acattttact tacccccgct tatattttaa ttttttgttg tcatttttct atattttaat
    16621 aatacagatt ctttgttaaa tgaatattag agcagggtta agtaaatgta aatattgtta
    16681 aatacatgta aaaacttaat tttatatgat ttattcttaa ttagattaac tttttaaagt
    16741 tactttaggc agatataaac ataatacaac tcatattatc agaagtcttc attgaagcta
    16801 tcaacctatc acatcctata attctttttc tagtttatta actaattcag caaatacatt
    16861 taaggcttcc tctatagatt cttttttcgt catatctaca ccagcagatt ttaattgttc
    16921 tagtgggtat tctgaccctc cactttttaa gaagtttata tatctatcta ctgcagtact
    16981 tccttctgaa agtatctgtt gacttaaagc acttgcagca gaaaaccctg tggcatactt
    17041 atatacatag aagtttgaat aaaaatgagg tattctcgcc cattctattc ctatttgttt
    17101 atctactatt gcagattttc cataatactt ttcatttaat ttgtagtata tattagtaaa
    17161 ttcatcagca gtaagtggtt ctccactctc taccctttga tgtacaattt tttcaaattc
    17221 agcaaacata gtttgtctat gaactgtagt tctaaattgt tctaaataat aatttaataa
    17281 gtatatagtt tcatccttag acttagaatt ttctaataaa taatttataa gaagtaattc
    17341 atttaatgta gatgcaactt ctgcaacgaa tatcttatag tccgaatata aaaatggttg
    17401 acttgttctt gaataatgac tgtgaacaga gtgccctaat tcatgaatta aagtgaatag
    17461 agaattaagg tcatttttat agctcattaa tatataagga tgtgaatcat aacttcccca
    17521 agaataagct cctccttttt taccttcatt atcatataca tcaatccacc cttcatcaaa
    17581 agcacttttt atgatcttca agtattcttc tcctaaaggt ttaagtgcct ctaatatgat
    17641 atcctgtgct ttatcatatg gtatatccat gtcaaattta tctgtcaaag gaacatacaa
    17701 atcatacatg tgtatttcat tcaatcctaa aaatttcttt ttaagctcaa catatttatt
    17761 tagattagga atacttttat ctatagctga tataagatta ttgtaaacat ctaaacttac
    17821 atcatctgaa aataaagacg cttctattgc tgaattatac tttctagttt ttgcgtaaaa
    17881 tatctctgat tttattccac cataaagcgt tgaggcaaat gtatttttat atttctcata
    17941 tgttgaaaat tcaccttcaa aagcgtcttt tctaactcta ctattcttac tttttataaa
    18001 taaagagtag tttgaatgag aaagcttaac cttattcccc tcctcatctt ctatctcagg
    18061 aaattccata tctgcatatg ctaacatatc gtatacattt tctggaattg tcgataattc
    18121 tgatgtagct gcaagtattt cttcctctcg ttcactcaaa gtatgtggtt tatctctcaa
    18181 tatatccttt atatattttg cataaaagga tagcttctca tcttttaagt attcacttaa
    18241 cttattctct tctatcgcca gtatttctgg aactatataa gaagtagcca cacctaaatc
    18301 agtagataac atttctgtct tagtggctct tccttgatta tcattaattc tagtatcttc
    18361 atgttgtttc atatgagtat atacatataa attttgaaca atcctagatg cattttcaga
    18421 gatatttaat actttatata agttctcctt actctcagaa agcttccctt tatattcttt
    18481 tacttcttgg ataagatttt ttactttact tatgtctttt tcaatttcct ctttactgga
    18541 gtacatctta tctatcttcc atttgtactt ttcttctatt gtacttctat cagttcccat
    18601 atcatacacc tctattttaa tttgttttat attttattca ctttacaaaa tatattgtat
    18661 atattttagt ccaaataatc tacatttatt actccttcta tcatatttat ttctttcata
    18721 acgtcgtgta agtgtattct cttagcataa gatacaaaaa aattaacctt ccagtattct
    18781 tcattacgac attctatttc catatttctt acgataagtt gatttttttc aaatttatca
    18841 tataaaaaag gtatagagtc caaattgctt aattttaata tcaacacatt cttttcttgg
    18901 ctaaataaaa acgtattgat tatcctaagc acaaataatg ctatcattac aaataatcca
    18961 cctagaattg ccaattcata ataaccatat ccaactgcta taccaataca tgcagttgcc
    19021 cacatcccag cagctgtagt aagccctttt actccacctt tattttttaa aattgctcct
    19081 gcacctaaaa aaccaattcc agataatact tgagcaggca ttcttgctgg gtcagcattt
    19141 gtatacaaat tataatcaaa gaataattga atcgaagtta ttgaaacaat acaagaacct
    19201 attgcaatta gtatatgtgt cctaaatcct gcaaactgtc ttattttttc tctctctaag
    19261 cctattattc ctcctattat tagtgctaat agtaacctta ttgctatctc cttacttcct
    19321 atatcaatca taatataaaa ccctcctgac ttaattacaa cttatgtcct acagataatt
    19381 tacacatcag aaatactatt ttatatctta atattattat taatatatgt aaaatatagc
    19441 catctgttaa tttttgatac aaaaaaaccc acaactgtgg gctatttgct taggtacatg
    19501 tatttattta ttaatatatc tattttctta cttgattcta ctaactcttc ttgaaatatt
    19561 tctggatatt ctatgtatgt atttatctct tccctaactg cttcaatctc attttttaac
    19621 gcttctaatt cattgttcgc cataataatc accactatta caaatttgta tatttttata
    19681 tacattttat caaacaatat tatattttgt caatattatg tcaataaaac ttactataaa
    19741 aatgctttaa tttaggtatt gtatacctgg atactaagcc tacaaacata ccagttacta
    19801 atgatgccat taacataaat ggtaagtacc ctatagcaag tatattttct atgattaaac
    19861 ttgcaactat taattgacct atattatgcc ctattgcacc tataatgctt atccctatta
    19921 gtgataaatc ttttatttta ctcgctaaat acataaataa aagactcaga taagctcctc
    19981 ctatactaaa tagtagatat gataaagggc ctgcaaatat tgatgacaaa attaccctga
    20041 tagtaacaat tataacagta tctttgacac ctaacaacat taatgaaatt aaagttataa
    20101 tattgctcaa acccaatttt gcccctggaa atataactat aagaggattt ggtatatatg
    20161 tctctaatac atataaagct aaactatacc ctatcataag acctaaaaat gtcatttttc
    20221 tagttttcat taagttaact cctccaaatt ttatctatca tcttacatta taatacaaaa
    20281 tatataaaaa cacctaattt agactaaaat atttttaatc tatttagaat aaatattagt
    20341 tatttcaatg tgcaattacc tctataaatc ttgacttttt gtatcatact tgttacaatt
    20401 ttaatgataa tgaatttcat taggaggtgt taataatgac tgtttacgat ttaaaaatag
    20461 gagaaaaagg atttattaat agtatagatg gagatattaa attatctaaa agattgcttg
    20521 cacttggatg tataaatagt actgaaatag aagttaaaaa ggttgcccca cttggagacc
    20581 caattataat tagatttaga ggctttgacc ttgcgattag aaaatctgat gctaaaaata
    20641 tttctttaca ataatagagg aggagctacc cgtgattaat gtagcattat taggaaaccc
    20701 aaatgtcggt aaaactactg tttttaatct acttacaggt tcaaatcaat atgtaggtaa
    20761 ctggcctggt gttactatag aaaaaaaaga aggatttctt ggaaaagaga taaaggttgt
    20821 cgatttacct ggtatatatg caatggatac tttctcaaat gaagaaaagg tttctaaatc
    20881 atatttagaa aatgaagatg ttgatgtaat agtaaatgta gttgatgctt ctaatttatc
    20941 aagaaacctt tacttaacaa ctcaacttat gcaatttaac aagcctatag ttatattgct
    21001 taatatgtta gacattgcag aatctaaagg agttaatata gatgccaaaa aattaagtga
    21061 agaacttgga gtaattgttg ttccaattat agcaaaaaag aaaaatggtg ttgataggat
    21121 agaagaagag attaagggag ctacgcaaaa agcttttata tatcagaaaa actttggaag
    21181 tgaaacagaa acatataaaa aattagaatc cattttatca agatgtacaa aaactacata
    21241 tacacaaaag aaatctatca gtgataagat agacaatata gtcttgaatc ctatattagc
    21301 atatccgata tttattggtg tattgttcct cttatttaaa tttacctttg actgggtcgg
    21361 aggtccacta caagagggat ttgctagtct tatagaggct tatatttctg cacctgttag
    21421 tgatatgtta tctaactcta gcccatggtt taaatcactt atagttgatg gaataattgg
    21481 aggtgttggt ggtactttac cattcttccc tttaatattt acattattct ttggtatatc
    21541 gttgtttgaa gatagtggat atatgtcaag aacagcattt ttaatggata aagttatgag
    21601 aaaggttggt ctatctggta aagcttttat acctatagtt atgggtatgg gatgttcttc
    21661 tccagctata atggcaacta gaacattgga aagcgaaaaa gatagaagag ttacagctct
    21721 tattgcacca cttatgactt gtggagcaaa gctacctata tatgcactat ttgtagctat
    21781 attctttcca cataatgctg ctttagtaac tacatcactg tatttggtgg gtatagttat
    21841 ggcaattgta gttgcattat tcttaaataa aacagccttt aaaacagagg ctgaaccatt
    21901 tatattagaa ttaccagaat atagaatccc tactatagat gcacttatta aaaatacttg
    21961 gaataaatca aaaggtttct tgataagagt tgttactgtc atgtttgcaa tgtcagtcgt
    22021 aatttgggga ctttcttcat tcaatgtttt tggatataca gaagatataa atgtaagctt
    22081 cttggctact ctaggtcata ttatatctcc tatattcaaa cctcttggtt ttgatgattg
    22141 gagaacttca gttgctattt taagtggtct tggagcaaaa gaaatcgtag taaactcttt
    22201 aaatatacta tatggtaatc taactgttgt cttaccaaca atatttaatg gagttactgc
    22261 atatacattc ttgatattta ctgctttata tactccatgt atagctgcac ttgcaactat
    22321 gaaaaaagaa tatggcaata aaatgatgtt tacatcattt gcatatcaat ttatactagc
    22381 atggataatg gcttttatag ttaaaagtat aggtggagta ctatttatgg gtaattcaat
    22441 aatagaagct ttgataggtg gaataattat agtccttgca ctctttattc tgtttaataa
    22501 attcaagtct acaaaaaacg gaaatgtctg tagtggatgt tctatttgtt ctggatgtcc
    22561 aagtgctaat tcttgctcaa gtaaggaaga cagcaataaa aaaaataatg cacactaata
    22621 tataaatatt ttagtttaaa ataagataaa agtgggtgtc tcaaaattaa ctttttagtt
    22681 catgaggcac tcttttttat agaaaatcaa atatattttc atcatttcaa cagtaaaaaa
    22741 gagcttatct agtaaaaaag aggcttatct ctattttgag acaacctctt tttttgttac
    22801 ttattcagtt tcttgctacc taacttcatg tggaacctag tatcttttgg aacacttggg
    22861 tctacacctc ttaatgtagg tatttcccca catattagtt gtataggtac tataaaatta
    22921 aatgtttgat agtaaggatt atctgtgata tcaatcttta aatttttatc attttctgta
    22981 acatctctac ctatagcaaa tacattttcc gtccaaccag aaagtacatc aatcatctta
    23041 gttactctag gttcttttcc tgtatctagt ataaatatag tagaatcact atttatagca
    23101 ttatatatac catgtataaa ctcttcaaac tcatacccag ttacaggtat tctcattgtt
    23161 tccaataatt ttaatgctgc ttctaatgta tctccatata tatcagagtg acctattatt
    23221 cttatttctt tagaattaac taacttttct ttgtttcttt caatccattg tttagagagc
    23281 ttatacactg cttcaaatct attaattgcg tctaatatct tatttatttc ctcattgtac
    23341 ttttcactag atattatacc tttttctcta gctatttgaa gacctaataa cattaagttt
    23401 aactttgtac aataatatcc ttttgtttta gctccagatt tttcttcacc acaatttact
    23461 gttaatatat aatcagaaat ctcatctatt aaagcattct tacaacctgc catagatgct
    23521 attttacagc ccttatcttc agctaacttc atagcgttgt atgtagaata actactccca
    23581 ccttgagata cacctactac tagggtattt tcattatcaa acttgaatgt atcttcagtt
    23641 atcataaatg gatacatttt cacaacatct atatctaata aattttgtaa atatggttgc
    23701 acttgcactc ctgagtgata gctagtacct gaacctgtta ttataatctt ttttagattt
    23761 gttctcttaa cttcattaaa taagctatct gcattagata taatttctct cattctaact
    23821 ggtgtttcta acatataatc ttgtattgtc attttataca ccttccttat ctatatattc
    23881 aataaattat aattaatttc tataacaaac ctatagcatg taaagcgatt cctgttacta
    23941 tagtgcctat agttaatttt gttacactta cctttctctt taacatgtag tatattacaa
    24001 atgcaaaggt taaaggtaac atattaggta ttattttatc tagtatatct tgtaaaatta
    24061 cttctgcacc atttaagttg aatttcagag gagtagttat actcaacata gttgcaacca
    24121 ttcctcctac aacgaataaa ccaacagttg tagccataga cattattttc tccataagtc
    24181 cctctctttg tattctttca agagaattta cccctacttg ataaccatac ttaagtccaa
    24241 atattctaag ggcaaatgct ggtatattgt ataatagtaa gaataatagt ggtccaaaga
    24301 tattaccttc ttttgcaagt gatactccta ctcctgctgc tataatccta aatgttcccc
    24361 agaataatga atcaccaatt cctgcaagag gtcccataag agctgtttta acagcactaa
    24421 ttgaactttc atcaaattct ggattattag cattctcttc ttccatagct gctgttatac
    24481 ctaatatagc aggaactact actgtcgttg tattaaaaat ctccaaatgc ctttctattg
    24541 cttttgcttg attttctttc ttattgtata gcttctttat tgcaggtaac attgaataac
    24601 agtaacctaa gttttgcatt ctttcgtaat taaatgcacc ttgaagagca aatgaacgcc
    24661 aaaacatact tcttaattct ttttttgtta atttattcaa agtcaatctc tccttccgtt
    24721 aaagtattga ttgctaattg aggattttga ttattttctt tgttattgat ataaaagttt
    24781 agtacaaatg ctacacatac tccaaataaa gctatagcag ttatatctaa tccaccataa
    24841 gatgctaaca aaaaccctat aaagaaaaat acagacatct tttttgaaag taacatatcc
    24901 ataagcaatg caaaacctac tgctggcaac aatgttcctg caacacccag tccgtctaat
    24961 ataactttag gtattgcatt tatcaatgct gtaactttat cagcaccaat cataattgct
    25021 aaaaaggttg gaactgatgt acttaaaaag aataatatta ctggcaacca catcattatg
    25081 gtaactttct taaaatcagc ttgtttagca tagaaaactg ccttttgaga aaagtacgaa
    25141 tttattattc taactaatac tccaagagat tgtgctaaga ctgctactgg tactgctact
    25201 gctaaagcaa cttctgcacc attacctgat aatatagcaa aagctgtacc taatacgcct
    25261 cctgttacta catctggagg agtagctgca cctattcctg caattcccat ccaaattaat
    25321 tcaagctgtg caccaatcat aattcctgat tttatgtctc ctaaaactaa tcctactaac
    25381 atacctgtaa ctattggcct atcaagcata gattgaccaa atatacgccc atctagtata
    25441 ccaactcctg ctataatagc taacatagta gcttgtacta acatattgaa cctccatttt
    25501 aacttagttt aatttacatt aaattacatt acatttttga ttttgttttg attttctaat
    25561 aactcattta cataaacaat tccatttttt gcttcctcta atcctgattt aactattagt
    25621 tttgtatcct cctcaactaa taaaagtata gataaaagca ttggtaaatt tactcctgtc
    25681 aataattcaa atttgaaccc ttgtaaaagt aagtttgtac atatgtttgc cacacttcca
    25741 ccaaaaacat ctgttaagac aacaaattct tcactatctt tattatttga taatatactt
    25801 ctaatttctt tctcagcatc atctgctgat ttatccgttg tcatacaaaa atactcaata
    25861 ttagttaatg aaccaattat taatttactt gtgtcaacaa gctctctgga caattctcca
    25921 tgtgttgcaa ttagaaattt tttcattctc tttctcctaa attattttat taaatctgat
    25981 agttgtttct ttttgtctct aggtaacatt ctaaattcta tatcaatccc taaatcacct
    26041 atttgtttaa agttatttat atcatcatca tcaacagcaa ctaaatctgt aagtttcttc
    26101 ttcccttcct catatcttaa tcctcctaca tttatttctt ttaatactcc tcctgaattt
    26161 ttagctacaa ctaaagcatc tttggtattt tttactaaca ctaatacatt gcttttcttt
    26221 gaagaagcaa atttttctac tatttctcca gattcctcta ccccccttat atataaagtt
    26281 acattgctag gtttcgctaa gttcagtgac atagctttca tttggtcatt ttttgcttca
    26341 tcatttgcaa ctagtattac atttatacct agtgatgttg cccatccata tgcaacctga
    26401 ccatgtatca atctgtcatc aattctcatc aaagatacca ttttccccaa atcctccatc
    26461 atacattaaa atttttattg tttatactct catttatcta taaaagcaaa aagtatgcca
    26521 aatactggca tactttttta aatttcattc acttattttg taatcttgta ttttactgtg
    26581 gtagaccata gataatatta taaatatatc ctatctctga aattggtatt tgcacactat
    26641 aactcttttc tagcacacta aatgcatttt ttattagttt tacttcacta tgatggtttt
    26701 tgattaacaa atccatatca gtatactctt caatttcagt ttgtctaact aatctttcaa
    26761 tcatacaact taagtgaaca tataaagaaa tctttttatc atttgaaagt tttttctttt
    26821 gaatattttc tatagcatta attccttctt ctattctctc tattatcttt tttgaatcta
    26881 aaatagttag actatctatt actctaatta atgaaaaatt ctttacaata gaatcattta
    26941 tgttctttat tttttcattt tctactactc tgctaaatat tctaaaaaca tcatcttctc
    27001 cttttcctga tattatatcc tctaataata taaaatctac cccattcaca tttggattgg
    27061 cagttcctac tatagccatc acatcataca tttcaaaaac tggttcttta tctcccatag
    27121 aaacaagact atcataatca taaggtatta ttttgacatc aactattcca attaaacttt
    27181 cggaaagtag atgttggatt tttatagcag tacctatccc tgtaaaacaa gatgatatta
    27241 tagcatattc tttatttttt tgaggataat acaattgtat attaggttta gtttcttcat
    27301 ccataatttc tgcaatctct tcaatgtttt taccttgcat taataaattt cctacttcta
    27361 gtgcagaaat agtggatatt tggtctataa ataacattgg gctatttatc ttattcttta
    27421 aatcttcata tataagatta agagagccca tatcaaataa cacaattaaa ccattcttta
    27481 tcctatctcc ctctaggtat ctagacattt gttttgatac atcagcaata gtagcatcta
    27541 taggcatatc aatagctgta aatatatttt tacccaacat ccgattacat acattcgcca
    27601 tactactggc agtagcatat ccatgtgcta taatagtcgc attaacttga ttaaatttca
    27661 ttcttataat tgttgattta ataaatataa tcattatgat tgtatctaca ttattaagat
    27721 acatatccaa tctattcgtt attaatgttg atattttact tattatagag tattctttgt
    27781 aactctcttt ttttaaatat attagtaatt tattttctaa agctttatac tcatcagaca
    27841 attgataaga ttcgtccttc aaataaagat aagacgaaaa tgccaataca ttattcccat
    27901 catattttat attgttatta acttctaaat ttctaaatat atcctgcatt atcgatatta
    27961 taagctccat aagtacgtta ttattttcat cattatttga aaagactaaa ttgtccatta
    28021 gtctaatcat tagattagaa ctctttttac taaatttttc ctttgttatt tcattttttt
    28081 ctagtctatc aaagtaatct aaacatttta tgaaactttg atagattata tttcttttat
    28141 ttcttccatt tgacatttca atactaaatt gtttaggaga aaagataatg cttttgcttt
    28201 taccactttg gaattgaaat atatctacat tttctatata aagatttcta ggaaggtctt
    28261 gaagagtaac aaaaatattt ccatttcttc tttgattttt tgcataagct cgtccacatg
    28321 catattttat tacattttta cattcaccta tattcccctt gaattttatc tttaaaatcg
    28381 catttaatgt aatttgagtt atctctatag gcaaaccaag taccttactt tcttcataaa
    28441 taaaatgttc tacaatcttt cttttttcaa tagtccctct ttcttctata ttgggaacat
    28501 aaatatttat gggtattctt cttaaaaagg tttgtaaaaa ttcagataga ctttctgttg
    28561 tagcaaatat taatcttacc ctagcttttc taattactcc attctctcca atcctagaaa
    28621 acgtacctgt atccataaat gtaaataact tttcctgacc ttcactattt aatctatgta
    28681 cttcatctaa aaataaaaca ccaccatctg attgttctat tagacctgct ctagattcat
    28741 ttgctccagt atatgctcct tttacatgcc caaataatag actagacaat aactccaaat
    28801 tattataata ctgcgcacag tttaacacat tgtatggaga atttttttct aatacattac
    28861 tgcttacact atatttgtat attatcttgg ctaaaaaagt ttttcctact ccactttcac
    28921 ctgtcagcat tattgtaagt ccgccatttg gataaagtac agcggttttt atttgttcga
    28981 ttacttcttt taagctattc tcatatccta tgatactatt aaacacagtc tcttgcttta
    29041 ataatttaca ctcattttta agttcatcta aattttcata aatacttttt tttgtaggtc
    29101 ctactacatc atttatttta tttttaggta aaaaaataac aggtctactg tttatcttta
    29161 taagattatc agattttact aaatcattta ataatctact tgattgagtt ctacttaaat
    29221 taaacttttt tactatatct tctgttatta tgccactttc ttttttaagt tcatcaaagt
    29281 tgttaaataa tacacattca tttacaaaac ttaaaatttc atccctcatg ttcaacctcc
    29341 aatacaaata tcaataatac tataatatta gtatatcatt tatatattta tgaaaaagat
    29401 aattatattc ctatcttgat tctcttttgt aaggttgtaa aatcaaactt tttataagta
    29461 taaaatctag attttttaac ataatctcaa ggttcttatc tttccatata tctgacatag
    29521 aatttatata agaatatatt tattgtattt attataataa aaagtaaaac attataggta
    29581 ttttaaaact tattagcgaa gaaaatattt gtaactttta ttaccttatg aatattattt
    29641 caaatttaaa aaggctataa ataaatttat
//
LOCUS       DAENCV010000042        29616 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAENCV010000042 DAENCV010000000
VERSION     DAENCV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.042"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(86..1744)
                     /gene="bclA2"
                     /locus_tag="KQD82_001851"
     CDS             complement(86..1744)
                     /gene="bclA2"
                     /locus_tag="KQD82_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG4380325.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGVNGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFNSSFING
                     TNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAKAVNTLT
                     SSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2102..2641)
                     /gene="hpt"
                     /locus_tag="KQD82_001852"
     CDS             complement(2102..2641)
                     /gene="hpt"
                     /locus_tag="KQD82_001852"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG4380326.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2969..4276)
                     /locus_tag="KQD82_001853"
     CDS             complement(2969..4276)
                     /locus_tag="KQD82_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG4380327.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4700..6475)
                     /locus_tag="KQD82_001854"
     CDS             complement(4700..6475)
                     /locus_tag="KQD82_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380328.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKVDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6472..6609)
                     /locus_tag="KQD82_001855"
     CDS             complement(6472..6609)
                     /locus_tag="KQD82_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380329.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6687..7256)
                     /locus_tag="KQD82_001856"
     CDS             complement(6687..7256)
                     /locus_tag="KQD82_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG4380330.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7440..8075)
                     /locus_tag="KQD82_001857"
     CDS             complement(7440..8075)
                     /locus_tag="KQD82_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG4380331.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8743..9624)
                     /locus_tag="KQD82_001858"
     CDS             complement(8743..9624)
                     /locus_tag="KQD82_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG4380332.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9744..10751)
                     /locus_tag="KQD82_001859"
     CDS             complement(9744..10751)
                     /locus_tag="KQD82_001859"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG4380333.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10771..11253)
                     /locus_tag="KQD82_001860"
     CDS             complement(10771..11253)
                     /locus_tag="KQD82_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG4380334.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11271..11738)
                     /locus_tag="KQD82_001861"
     CDS             complement(11271..11738)
                     /locus_tag="KQD82_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG4380335.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11753..12511)
                     /gene="prdD"
                     /locus_tag="KQD82_001862"
     CDS             complement(11753..12511)
                     /gene="prdD"
                     /locus_tag="KQD82_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG4380336.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12591..13316)
                     /gene="prdB"
                     /locus_tag="KQD82_001863"
     CDS             complement(12591..13316)
                     /gene="prdB"
                     /locus_tag="KQD82_001863"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12864..12866),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG4380337.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13354..13638)
                     /locus_tag="KQD82_001864"
     CDS             complement(13354..13638)
                     /locus_tag="KQD82_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380338.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13719..15599)
                     /gene="prdA"
                     /locus_tag="KQD82_001865"
     CDS             complement(13719..15599)
                     /gene="prdA"
                     /locus_tag="KQD82_001865"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG4380339.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16028..17788)
                     /locus_tag="KQD82_001866"
     CDS             complement(16028..17788)
                     /locus_tag="KQD82_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG4380340.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIYEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(18195..20342)
                     /locus_tag="KQD82_001867"
     CDS             complement(18195..20342)
                     /locus_tag="KQD82_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG4380341.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDSQTPDVEKPSDGQMPDVEKPLDEQTPEEEKPLEEK
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSKDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGGEENELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCDKLIERAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPP
                     VPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPSVSDTVEKTPELSRDDTIIK
                     SPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     regulatory      complement(20924..21009)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(21413..22726)
                     /gene="prdC"
                     /locus_tag="KQD82_001868"
     CDS             complement(21413..22726)
                     /gene="prdC"
                     /locus_tag="KQD82_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG4380342.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(23115..23879)
                     /locus_tag="KQD82_001869"
     CDS             complement(23115..23879)
                     /locus_tag="KQD82_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4380343.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            24170..24373
                     /locus_tag="KQD82_001870"
     CDS             24170..24373
                     /locus_tag="KQD82_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG4380344.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            24564..25100
                     /locus_tag="KQD82_001871"
     CDS             24564..25100
                     /locus_tag="KQD82_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG4380345.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(25104..25970)
                     /locus_tag="KQD82_001872"
     CDS             complement(25104..25970)
                     /locus_tag="KQD82_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG4380346.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            26118..26363
                     /locus_tag="KQD82_001873"
     CDS             26118..26363
                     /locus_tag="KQD82_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG4380347.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(26477..27775)
                     /locus_tag="KQD82_001874"
     CDS             complement(26477..27775)
                     /locus_tag="KQD82_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG4380348.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(27908..28939)
                     /locus_tag="KQD82_001875"
     CDS             complement(27908..28939)
                     /locus_tag="KQD82_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG4380349.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
BASE COUNT         8850 a         5542 c         3398 g        11826 t
ORIGIN      
        1 tcatataaac ctattaaata ttattctatt tattggtatg tggaattata ttgtttatta
       61 ttttatagtc ttttcatcat ataaactatt gtattctata aactgataca tatccagctg
      121 cagaagtacc tgtcgtgcct gaaccaaatg taaaacttaa agtaacagct gctgctctag
      181 catcaactaa aaagcttaaa cttacactta catctgatga agtaagtgta tttactgctt
      241 ttgcaataaa gttattttga gctactccat taaagttagt agtaattgac ataaatcctg
      301 caagtgaaag tgtggcacgt ataccgaaag atacattgta tatccctcca actgcaagat
      361 ttatagtact acttgttgga aaagttatat tagttccatt tataaatgat gaattaaatg
      421 tcactaatgt attattcact aaacttgaag aggacactgt aaattgtgca ttgtttgctg
      481 ctaacactcc tgttgctcct gtcgcccctg ttggacctgt tgctccattt gctcctgttg
      541 ctcctgtcgc tccatttgct ccggttgggc ctgttggtcc tatcgctcct gctactccat
      601 ttgctcccgt tgctcctact gctccggttg ggcctgttgc tccatttgca cctgttgctc
      661 ctgtcgctcc tgttgctcct gctactccat ttgctccggt tggaccggtt ggacctattg
      721 gacctgctac tccattcgct ccggttgctc ctactgctcc cgttgggcct gttgctccat
      781 ttacacctgt tgctcctgtc gctcctgttg gacctactgc tccatctgct cccgttgggc
      841 ctgttgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt gctcctgtcg
      901 ctcctgttgg acctaccaaa ccatttgctc cggttgctcc cgttgggcct gttgggccta
      961 ccaaaccatt tgctccggtt gctcccgttg ggcctgttgg gcctgttgga cctaccaagc
     1021 catctggacc tgttgctcct actgctcctg ttggacctac ctctccatct gctcctgttg
     1081 gacctgttgc tcctattggg cctgttgccc cagttgttcc tgttgctcca gtcgctccta
     1141 tacttcctgt tggacctgtt gctcctgtat ttcctgttgg gcctgtagca cctgttactc
     1201 catttgctcc ggttgctcct gtatttcctg ttggacctgt tattccattt gctccggttg
     1261 ctccggttgc tccagttagt ccggttggac ctgttgctcc tgttgctcct gtattccctg
     1321 ttggtcctgt tattccattt gctccagtcg ctcctgtatt tcctgttggt cctgtagagc
     1381 ctgttattcc atttgctccg gttgctcctt tgtttcctgt tggtcctgtt attccatttg
     1441 ctccggttgc tcctgtattc cctgtcggac ctgttattcc attcgctcca gtcgctcctg
     1501 tatttcctgt tggtcctgta gaacctgttg ttccatttgg tccagtcgct cccatatttc
     1561 ctgttggacc cgttattcca ttcgctccag ttgctcccgt atttcctgtt ggtcctgtta
     1621 ttccatttgc tccggtcgct ccggttgcac ctctaggccc cgtcggtcct gttggaccag
     1681 tagcacctgt tggccctgtt ggacctacat cttgatataa acttggacct gaaatatcac
     1741 tcatataatt aatcctcctt ttttaaagtt agagtaatac tctataaata ttgagtatta
     1801 ccctatataa ttatatgaat cgacaatttc ttatgtgata atgtatctat ttgcttatat
     1861 caacatatat gctttgattc ttgaatttca ttactttttt gtaaaatctt ttacaattat
     1921 taatacaatt aaaaaactca tttcccatca attatttaaa atttttttat tatgttataa
     1981 aaaatagata attaaattgc ataaaattaa tttaatacta ctttaattac tttttaaata
     2041 aaaaaacctc aacacgatta ataaaaatca tattaaggca tccacaaaat tatataattg
     2101 cttatctatc ttcatcagta acattgaata tataaggaac atttctataa taatcaccaa
     2161 aatcaaaccc atatccaacc acaaacttat cttctatttc aaatccacaa taatctggca
     2221 ctaaatcgac ttttcttctg cttggtttat ccaaaagaac acagcatttt aaactagcag
     2281 gatttttagc ttttaaatgt tttaacacaa aatccatagt aagtgcagaa tcagtaatat
     2341 cgtctacaac caaaacatca tatcctgcta tatcagtagt cacatctgaa actactttaa
     2401 cctttccagt actttcttca ttgtttccat aactagatgt agtcataaaa ttaactctaa
     2461 gtggtaagtt tatatttcta actaagtctg cacaaaatat aaagctacct tttaatagag
     2521 aaactaccat aagattttta tctttataat ctttttctat aattgctcct aattccctta
     2581 atttatcttt aattttttct tctgaataca atacttccca tttttttgtt tctatatcca
     2641 taaaaagacc tccaattgta ataatccttc ttaattttat aagagattgc tcactttttc
     2701 aatactctta ttcaatatta gtcaatatta ttgagttttt atgttcattc aacaattatt
     2761 aacaatgtat cttatatttt aagtagttca tatatccaca ctaaattaca tactatatat
     2821 tttagacaaa aaaagaaaaa acttgcacta acaagttttt tctattatac ataaatttat
     2881 acataatcat attttattag agggtactaa ttaaatttat ttatgctatt tttgttatcc
     2941 ctcataatac aaaacatatt ataaagtact agtcaacttt attcatatca ttcattacat
     3001 ttaagattat ctcatctgtt atagacatac ctttgtcact tactctacct aagttttgta
     3061 tactttgctc aacacttgaa ccaactatac catttagtgg tggtacaaaa caatcttgct
     3121 tagctattat agcactttgt atagctgcag atgctgcaga agataatttt aatgcacaac
     3181 cagctttcgc accgtcacat atcattccac ttaagtcagc tatcatattt tctatagctc
     3241 cttctatttg cttttcagta ccattcataa gccaagagat accagcagtt gctccagtag
     3301 aagcagcaac accacatcca catacagcag ataatcttcc tgtatagttc ttaatataac
     3361 cagttactag atgagatata gctaaagctt ttgctagtct ttcatcagat tgtgggaatt
     3421 ttttattata agcaacgatt ggaagaattg ctgttaatcc atgatttcca cttccatttg
     3481 aactcataac aggcattttt acccctgcca ttcttgcatc cgaagctcca gcagttaaca
     3541 tcattgcata atttataaca tcattaccta aaagaccttc ttctatagac tttttaattc
     3601 catatccgac accaatacca gttttttgtt ttaatccata ttcagccatc tcttcattca
     3661 tttttactcc atctagtaaa aattctatat ctttaaaatc aattccttct acatttttta
     3721 ttaattcttg tatagttact gtatccatta aactttcagc tttttctgcc acatcatttg
     3781 aagctgatac ttttggttca ttgtctaata atacttctcc atctttttct aagaacgtaa
     3841 aattatcatg tttagttctt attctcactt tagcaatatg attttttcct tttaacacaa
     3901 cttctataaa tactttctct cttgtatcag caggtgttat tgatagtgga gtattatcca
     3961 tataatcctc agctatttta acttcctctt ttgttagagt ttctaacaca cttaatccat
     4021 tttcagaatg tccacctact atacctagag ctgctgcaat ttttaatcct aatctttcag
     4081 tacctggtat acctacgcac ataccatttt tatagatact aggactaact aacatacgat
     4141 tttcaactat ttcttctcct aataattcct ttgcctttgc acatgctaga gctaaggcta
     4201 ctggttcagt acaccctact gctggtttaa cttctgcctt taacatttcc actaattctt
     4261 ttcttatatc tctcattata tataaacctc ccaattaagt ttagatttag tactcaaata
     4321 attaattaaa taatttctta tctatattta tagcaaaata catgccatat tttttatatc
     4381 tttttatatt tttagtctta tattctataa ccattggtat gactgcttct atttatgtat
     4441 ttttttattt acatagagta tttatattct ttaaaatgga taaacatatg acaatttcgc
     4501 aaaaagacaa aaaaaagtct caaaatgaga ttctatatca ttttgagacg gctcaaatgt
     4561 attatttttt aacttataaa aaccattttt taattcataa aaaaagattc tgcaaaacac
     4621 ttatagtata agtttaaagc tttctttaaa gttatgaatt tagaaaaatt taaattttat
     4681 atagcttttt attatattac tatgaaatta tattgtattt ttcaattttt ctatataatg
     4741 tagctcttga tattccaagt gctttagctg ttaattcttt atctttttta taatctttgt
     4801 acttatttaa agcttttatt atctcaatct tctctagttc atcaagtgga attatcctat
     4861 cgcttttaac ttctaactct tctacagagg tattcttaat tttttgagga attacattca
     4921 ttgtgattat tgaagaagaa gacatattca cactatattc aataatattc tgcaactctc
     4981 ttatattccc tggccactta tattccatca tagcatcaac aaccctgcta tctatgtcaa
     5041 taacattttt atctaatttt tttgaatact ctttaaccat atgcttgatt ataagtggga
     5101 tatcttcttt tctctctctt aaacttggta attttatagg tataacattt aatctatagt
     5161 ataaatcttc tctaaaagtg ccattttgaa ccattttttc taaatcttta tttgtagcag
     5221 caataattct tacatctatt gaaacatttg atttacttcc aattttatta agttcctttt
     5281 cttgtaatac tctcaataac tttgactgta aatgcaaact catatctcct atttcatcta
     5341 aaaatatact tcccttatga gctacttcaa acattccaag ctttccacct tttttagctc
     5401 cagtaaaagc tccctcttca tatccaaata attcactctc taataaatta tccggtatag
     5461 cagcacagtt tacagctata aaaggattat ctactctatt gctatggttg tgtatggctc
     5521 ttgcaaatag ctccttaccc gttccacttt ccccagtaat aagtactgtt gatgttgatt
     5581 tagaagctat tagggcttcc tttttagaat ttcttatagc tacactttct ccaattatgt
     5641 tttccaattt tattctataa tctttattca tcttattgta gttttttata gcatctcttt
     5701 tatctataaa atcaataaca tatcccttta actcattttt taatactatt ttattgatat
     5761 tgtatattcc cttcgaatca tatccatatc ttttataaaa aaaactacag gatttgtcct
     5821 tcttaaagtt atttatagaa ggtttcttta taaaatctaa ttcgacaaat atgtttttgc
     5881 cagttatact atcttttata ttgaaaagct ctttaaattt tgaattatat ttatcaactt
     5941 ttccatccac atcaatagac acaacagctt tgtccatatt gtttaatagt gtctctaatt
     6001 ttttcttttc taattcaagc tcataagttt taatttgtgc ttttaattta ttagatatta
     6061 ggtctgccat tttgcctaaa aagtttataa gaccactttt attattttct ataatttgtg
     6121 cttgttcttt tgtaaaggct ataaggccta taacaccatg agaatcgcca tcacatataa
     6181 taggacaaca cacctctgca aactctacac aagccccact tttatagcat tccctacata
     6241 tatcactact ctttgggtca tcaattagta ttgtaatctg ttctgtaagt gattttttaa
     6301 aagccgtata tttatctact ttctcaccta ttttgtctat atatactcct gtacctgcaa
     6361 tcctaaataa ctccttattt actatagtta catctatatt gagtactgat actatagctt
     6421 cagctatatt ttgaatatca gattttatat attctaaacc tatcataatc atcattcctt
     6481 atttttcttt aacataagta tttgtcttat ttctttaaga atcaagtata tcttatttaa
     6541 agtaaattgg attgacatca ggattacaaa gagcataaca attattatta actcataatt
     6601 tatttccatc taaacatctc cttataacaa aagactaggc ttaacctagt cttttatatt
     6661 atatccatat ttatatttta actcatttat cttctttttc ccacataaat atattttaat
     6721 ttatttgact ctttaatcat accatcaaag tttaatcttg aaaatatatt aaatacttca
     6781 tgtggatagt gaatctttgc aacagaacca caaaatttat gtttaagata gtttaactta
     6841 acttttaaat tctttaaatt taaatcctct aatataacct ctccattgcc ctttaacact
     6901 ctttgtattt cagatgaagc tttttcacaa tctgtaataa cattaaattg gtcaactatt
     6961 attatcttgt tgaactgatt atcatcaaat ggtagaaact ctaacttacc tttaactaca
     7021 ttgcaatttt cattatacac tagagaataa cagacatctt cataattact attctccagt
     7081 atagttacag aagttccaag acctctcaat ctcttgccta ggtttcctat atgaccaaca
     7141 agcaatattt tatcacctac attatactca attgtatcca aaaaacattc taattcatca
     7201 gatgtatatt tagctttaac cggctgttta tttatctgac ataatatgct tttcacaatg
     7261 cttcacctct tcatttcctc aattataaat tatgtacaga ttacattata ataaatggcg
     7321 agcaactatt caagagatat ttcattagta tttttttaaa caatctttat acattttaat
     7381 atgatattat accaaattta ttaggatttt attcataaaa atcagtctat ttttcatgtt
     7441 tattcattgt tattttcatc caattttttt attttcgata tggatacttc atatgccatt
     7501 tttttgacca cattattttc atctattttt ttctcatatt ctctactttg aaccctaccc
     7561 caaagctgta ttctatcacc aacctttaga ttctttgcaa atttagcatt tctaccccaa
     7621 actattgatg gtatataatc agatttatta taaggtctgt ttatagcaac taataaatcg
     7681 gttatttccc ttcctaaagg tgtcttccta tacacaggtt ccttacatac atatccatct
     7741 aaaaatatac tattagggtc tttattttcc tcgtctatgc tttttatttc tctgacaaac
     7801 actgtaagca ctaatctatt tttattattt aatgttttgt tatatgatct taattggcct
     7861 acgacattga ctaaattttg tttatcaagg tcaatatctt ggagaagtct ttctgataca
     7921 gtcattggaa gtatatcaaa agaatcgctt agtctgttta tttttatttt catattataa
     7981 aatttttctc caaatatctc atggctaaat tctagcttat tgtctaactc ccctcttaag
     8041 tttactacgt tattctcatc tttaacagct atcatatttt atccctccac aattgttaat
     8101 tcttatattt ctggtatcag cttatataat aaatatattt ataagggatg tattttatta
     8161 caaatctttg attgctgttt aaaagttttt atttagttat aaatttcatt taatatttca
     8221 cttttataat tataaaatta ttatcttttt gtaccacata tactaattaa actacctatt
     8281 tcagttttat attaattata aaaatcctat tatatagtta ataagtcata tataacttaa
     8341 atttattcac tctatataag caacttcaac gaattaaatt tattcaaata attcagatga
     8401 atattgagtc attataaact tatttttctt aattataagt tatattagac ttattacagg
     8461 ttatattttt attaatctgt attaatatat gaacatttat tgaacttatt gattataaaa
     8521 tttaaatatt tagctttaaa ttatgcttta ttctttaaaa atacaacgtt ttttacatta
     8581 aatctactaa atcactatat atattacata attggcatgt taattgcttt atattaattt
     8641 agtaaataaa aaactgccct aaaatattaa ttcaatattt taaagcagtt tataatcttt
     8701 attattagct gtaatattta ataaacacta agtagtcttt aactaagcag tctttttgtt
     8761 tttatttgca gccattatca ttgtagcaga agtataagct ataactacta ttactaatgc
     8821 ttttaatata tccattggta gagtttttac tatatatgca gctacgaaaa ctcctattaa
     8881 accacatata gctattataa ctgatgcttt tttagtatat gcatcttctt ttatgaattt
     8941 catacttgca actggcatta ataatgcaca agaacccata actattggga atgcaacttt
     9001 tgcagacatt cctaagaaat aaaccattgc catacctgga gcatataaac ctataccagc
     9061 tgtcattaaa gctcctagta taaagttacc tattacacct attactaatt taacaccagt
     9121 aagacctaat tcatttcctc cacctggtaa tacgttaatc caaggatgac ctaataacat
     9181 taatattgct gttaatgcta aagttacacc cataacaagt tgtatttttc tctcgtccat
     9241 cttagctatt attcctgcac ctatatatga accaactgct gctgctacaa ccatagatac
     9301 taaagtaaca ccatcaactg gtatgattga tgtaaatatg aatgcttctg taacaactgg
     9361 aatagtatgt gctacgttta atgtaccagg cattttttta tctggtatat ttaactttaa
     9421 agcctttgac attgctattg ttggagcaaa tgaccctatt cctaatgtat caaagaagtc
     9481 tgttataaat cctgttacag caacttttgg agtactttgt tcttctagtt tcccctcttt
     9541 gtttgcttta gctacgtctt taccaaacac aactgcaaag attgctgcga atactacata
     9601 taatgccttt aagattgcta ccataatcca tcctcctatt tttttatttt ttcatatatt
     9661 ctgaatctta tagcctaaaa gctatagaat agcaaccgac aagtggctac tattctattt
     9721 taatttccta atttatttat tacttattta agaataaatc catgtttaag tgggtcttct
     9781 tcatctatta caaaatgatt aaatccagtt atataagcag aaccagttat tttaggtact
     9841 acagcattga aatctgcaac tttagtttct tcaactattt cacctttgaa taaagttcct
     9901 aatatacttt catatacgaa tttttctcct acttttaatt cacctttagc atgtaaagta
     9961 gctaatttag cacttgttcc agttccacat ggagatctgt caacttgacc ttgaccaaat
    10021 ataactacat ttttgtaagt agcttctgga tgagttggct catcatatat ttcaactaag
    10081 tctacagttt ttatatgagc taaagttgga tgttgtattt ctattttctc atttattata
    10141 tctctaagtt tcatagctag ttcagttaat ttaccagcat tttgaggttc gatttttaaa
    10201 cctaattgac ttgcatgtat tatagcaaag aagcttccac caaatgatat gtcaaattta
    10261 acagttccaa cacctggtaa gtcaacttca acaccttctt tatataagaa agctggtacg
    10321 tttaagaatg atacttcttt agcttttcca tctacaactg taacgtctcc tcttattatt
    10381 cctgctggag cttccataac tacatgagtt acaggctcta ctgctggaac tacacctgtt
    10441 tctatagctg ctgtcatagc tcctattgta ccatggccac acatgttaag gtatccgcca
    10501 ccatccatga agattattcc aaagtcagca tctgggcaac aaggttgagt cattacagaa
    10561 ccaaacatat cattatgtcc tcttggctct aacattatag cagttcttaa ataatctaga
    10621 ttttcctcta aatattcttt tttctcaggc atagaatttc cttttatgtt aggtattcca
    10681 cctacaacta ttctagttgc ttcacctgct gtatgagagt ctatagcttg tatacttctg
    10741 ctaaatttca tatcaaattc ccccttaggt ttattttgcg tctttacgca tttttaattt
    10801 taggaaagcc aatgcttttt taacatcaac ttcatccatg tgattgtctc caactacttc
    10861 tgtttctatg tagcctgttg tctttttata atcaattatg gtagctttat ccaaatatgg
    10921 attggtaatt actaatccat ctgctcccat aatagtaaga cctactgtag gtatatctga
    10981 ttttacaaac tctgctatag catttgtaaa atcaatatgt tggtttcctg ccatatctaa
    11041 agttataatc actccatcag gtcgcaatga ttcaatccat cttccaagtc tagttgctaa
    11101 aaatgttttc cattcattgt gctctgatgt cccaactaca accactccta acaagtctat
    11161 gtcagaatca ttatctatca aatctagtaa cgggtctcta aatatatgaa gtgatgtgtt
    11221 tttcattgat gtagaaattc ccacaaaact cattccctta cttaaatttt ttatccatgt
    11281 tttctcattt taagttttaa aaatgccaaa gcttttttag catctatttc atttgtgttg
    11341 ttttctccaa ctacttctgt ctcaatacct tcttttgatt tgttcatatc aactatggta
    11401 tccatatatt gattgcttac aacaaactta gcttgagtcc cattaaatgt aactccaact
    11461 acaggtatat ctctttttcc tatttcctca aatgtgttag cgtagtcaac atgtgagttt
    11521 ccccaaccat ctgatgaaac tatagctcca tctactctca tggcttccag ccaagcggcc
    11581 gttctttgtc ccacaaaggt tttattttca tttccatctg gagttcctac tactattact
    11641 ccaagtaaat ctacatcctt atcactttca actatgtcta aaagtgggtc tctaaagtga
    11701 tgaagagatg tttcttttgt tgaaggtcct attcccataa tatccacctc tcttaagtca
    11761 tagctctaag ggcaccatct ctgtattcat taggagatat aatcataggt acatttccca
    11821 tatctataat tgatgttcct ccttcaaatc cactaggttc ctttgagaat aactgattgt
    11881 catacatagc accttgacct gctatttgtt ttactatgac aacctttttt gcatttggtc
    11941 gaattttgtc aaaatattca cgaacttgag tagcatttct tccgtcttcc attttcaaag
    12001 acgctcttat ttcttgtata aaatcatcac atgctctgaa tgctgcaagt ggaagtgttc
    12061 tttcaaaagg taaacctccc tttaaagtta catctacatg gattatataa tcttcaactg
    12121 atggagttcc atatctccca aataccattt ggtctttcaa aattccttct gaagaaccaa
    12181 attcatgcat ttgatttcca tcttcatctg caccagttaa cattacatat acacccgtta
    12241 aagtatgtgt aataccctct cccagtctgc ctaaaacttt tgttgatatt ggtataatgt
    12301 ccataatagt attaatttct ctgtcataat ctccaggttt tataatgtct acttttatgt
    12361 ctgttattaa ctcttctctt tcttttattt catctatact tgataaatct attgtaagta
    12421 catctttctt tatagaagtt tttgaaccga aattaacttc attcatatga aacggtttta
    12481 taacaagtct tctcattatt ttttcttcca taatatccac ctttgttctt aaaacccctc
    12541 tcctaaccgt gtaggagaag ggttctatat tttcaaatat aaattacatt ttaaacgtga
    12601 gctttatatt cgtatggtaa tggaactatc ttaccagcag atggtattgc aactaattgc
    12661 tctaatgtat ctcttaatat atgtgtttgc atttctatat tatgtggttc accagcatta
    12721 gcacccattg gaactaatgg agcaactgct ctaggagttc cgttttgtct aactactgga
    12781 ggaagagctg ctattattat tgtaggtatt ccagcttcct ctattgctct ctgcacgatc
    12841 acggcagttc tatggcaagt acctcagcca gctgtcataa ctacaccgtc tactccctct
    12901 tccttaagtt tagcagcgat agcaggacca gtttcttcag tgaagacatg ttggtttcct
    12961 ccaccaccca tgaatgcata gtgcatagga gctactcctt tgataaatcc ttctgcagct
    13021 aattcatgta atctatctat agggaacata cagtttatat ctttgtttac atctccattg
    13081 tcatatccac catgtgatac catcatttca tcaactgtag ctgtattagg tatagctcta
    13141 aatgtagtat ctcctgctag attaaatctc ttgtcagatt ttaaatgaac accagctgct
    13201 gtagctaaag ctatagtcat atcttttaat tctttagtta caggagtcca aactggagga
    13261 ggtgttattg gaacgaatat ttcagattga agtccttgta ctgttgtaag gctcatatat
    13321 ttcctctccc ttctattttc aaacgattat ttattatttt tcttcttgtt ttgattgcat
    13381 ttctgcttgt ttttcttttt ctctcctaat attttctaca tactcttgat taggctctgg
    13441 acttaatacc tcaggatagc ttagcatgaa tccaacttct tgacctatct taagttcgta
    13501 gtcagttata agcattctat taggtatttt aaattgtcca agtctacctt ttaggtctat
    13561 agtcactcca ccacctttga tgtcaactat aataccttca taaaaagtct ctgatgagat
    13621 atattttaac ctatccatta taattctacc tctctatact gttttaaact tggatctaaa
    13681 tttatgttat tgtctaaatc ttagttagta tctaaatttt agttttcgtc tttttcgtat
    13741 atttctcttc tcttcttact cattggtaga gattgctcat tctcttctaa tactattttt
    13801 tctcctgtaa ctttttctat agcttctatg ttatttaatt taacatttgg attccatttt
    13861 ctttcaggag ctttaacttc tacaccttct atagcatttt taagcatagc cattattcta
    13921 acagcatctt ctggagctaa agtgttgtta gataatattt cattctctat accttgctta
    13981 gacttattgt tgtctaccat gtgagtcatg tatttattac caacaactag agcaccttga
    14041 acagctgaga aacttacacc aactactggt atacctctca tacctatttg ctctatgtga
    14101 gatgcgaaat ctatatggtt gtttccgaaa ccttcagtag ttactacagc tccatcaact
    14161 tccatagctt caactaacat acctaatctc ttagatacat agaatttctc agcatttact
    14221 tgtggagaac caacaaatac aacacctact aaatctattt cttcgtcttc catagctctt
    14281 attactaatg gctctctcca gtaatgtctt gacatttctt ttgatgcagg tcctatacaa
    14341 gttaatgcat ggataccacc atctaatact tcaagtggag ataatacaac tggcatgttt
    14401 cctaagtcaa cgtttggttg agctcctaat gtaccaactg gctcaactgg taatattagg
    14461 ttatcatgca ttgcaccttg tcccattatt tcttttataa caacaacttt ctttttacct
    14521 tctcttctgt attgctctat tacttcttca tcaactacta aagagttgtc aactttcttt
    14581 aatgcttctc ttatttcttg agttacatag tcaaatgcac ggtgagcagc taaaggtcct
    14641 ggtctttcca tgttagttcc tgcttttatt gttacttgac ctttgatgaa tatttcacct
    14701 ttgtcagcag cacctggtct accccacatt atagtagtat ttaactcacc ttctgaagaa
    14761 ccaaattcac ctatttgaac tccatcttca tctgtaccag ttaataccat tacagctcca
    14821 tctataactc tagttatacc tgaacctaat tcgccttctt ctttagtagc gattggttgt
    14881 atatctaata cagcttcact gtaagtttca tatttatcag gagttattat ttctaacttc
    14941 atatctacaa ctaactcttg agtttcatta gcttctttac atatttcttc tgcgtttctg
    15001 atgtataaag tagttccttc tattttagtt tcatcagcaa attcaacttt atctatttta
    15061 aagtgttttt tagttaagct tcttaaaagt ttcttttctt caacttttgg agctacttct
    15121 gctgctgcac ttgccactgg agcagcaacg tttgctactg gagcagcaac tcctgcttga
    15181 ccagctactg ataaaggtat ctctaagctt atgttcttac cttcacctat gtgtatttta
    15241 actgtttcac ctgcaactcc tgtgcttact ggaactactg ctgcttctga tactggagct
    15301 gcttcttcta ctgtttcttc tactacttct tctttagcct ctcctgcttt gtatccttct
    15361 acgttatctg tagtcattgg agaaagtgca tctaaagttt cttttaactt agctcctagt
    15421 acttgaccta tagttaatcc attttctggt attgttaata atccagaatc ctctaagtct
    15481 gcaaatattg ctggatcttc taagttttct ggcgctataa ttgttcccgc ttcaaatcta
    15541 caacaacaaa ctgctgggtc atttgcatgg gcttgagctg tttctaaagt tattgacata
    15601 gtataaaata cctccttaca ttattttctt atttattatt ttaaatctca attcctcaac
    15661 aggtaacccc ttaactgaga aattaagtaa attaaaacct acataatttg ttatgattaa
    15721 tattattttt ttctcactta aagtcacttt atattataag caattcctat gccaacttat
    15781 taacaaaaag attttttcta taatagattc ttcatctatc gctattttta gccaaatatt
    15841 atataaatat ttatttaaaa tatgttattt tttgtatttg taaacatttt ctcaaacaaa
    15901 aaaagtgtca attaatcaac actttttcat tttttttaac acatctatat acacaacact
    15961 tatacaagcg ttggctttac taggttttaa attaaagttt atcattttgt caaaatttag
    16021 tcatgtgtca tattttagac acatctattt tgtattggtc tatcttataa tacaaagttg
    16081 ttctgggtat atttaatatc ttagctgctt tagctttatt tccattagac attttaagtg
    16141 ccttggttat attctttatt tcaatttttt gagttgcctt cgttaaatct aaaggatact
    16201 cttcttcttc agtactatta tttgtagaat ccatcatata acttggtaca tcatccacat
    16261 ctattttaga attttgtgac aatacaacaa tattttcaat cgtatttttt aattctctaa
    16321 tattaccttt ccatctataa ttctgaaata tatctattac atctttgcta attgtaagaa
    16381 caggcttatt attttgttta catatctcct ctaaaaagct atgaaccaat aatcctatgt
    16441 cttcttttct ctctctaagt ggaggaatct ttatttcaac aacatttaat ctatagtata
    16501 aatcttctct aaatttttct gcctttacca ttttactcaa atctttattg gtagctgata
    16561 ttattctagg atttattttt attgtggtat ctccaccaac tcttcttatt tctttttcct
    16621 gtaatactct aagcaacttt gcttgcatac taagaggtaa atcggcaatt tcatctaaaa
    16681 atacagttcc atcttttgca agttcaaata tacctattcg acccttttta cttgctcctg
    16741 taaacgcacc tgattcatac ccaaaaaact cactttcaaa taactcattt ggtatggcac
    16801 tacagttaac aggtataaat tgaccttttc tctcactata atcatgaata gctcttgcaa
    16861 atacttcttt acctgtacca ctttcgcccc aaataaatat gctactattt gttctagcca
    16921 cttgttttgc aatcgctttc gatttttcca atttataact tttacctaaa atcttatcaa
    16981 aactaccttt agataaattt ttgacttcgt tctttagata ttttaatgta tcatttactt
    17041 tttcaagttc aagagctaat atcttagctt ctgtaacatc aacttctgta catacaacac
    17101 cgataaattc ttctttataa aatataggat ttgcatgaac taatgcatac atattttctt
    17161 ttttatctgt aaaatacaag tcactcattc caactttact atttagtact ttttctgaaa
    17221 tagtattttc caaaaattca ttcatcggtc gaccaactat atcttcagac tttatatcat
    17281 acctttcctc cataaactta ttccacaaga taacaacacc ttcgttattt atagcacaaa
    17341 ttccttcttt aatttgacct ataatatact tcaaagttaa tccataatct tctaaatgca
    17401 tatatagata gtccctaatg tgctcttgtt ttagaactcc aataatttta ttatctctaa
    17461 gaacaggaag aatccctata ttttttaata tcattatatc tctacattca tctaaggtta
    17521 aaccttcgtt gacatatatt acatccttct tcattataag ttttactggt tgaccttcat
    17581 actttttatg catctcataa agattatgta tatccgtcat ggatataatg ccttttaatt
    17641 ggtcagatga atctattacc attagagttt ttgtgttgct ttttatcatt tcttttattg
    17701 catcttcaat tcttgtgtct tcgtatatag ttgtaaattt tgtatccatt acctcttcta
    17761 ctttttttac ttctggtatc gaaaacataa agtcccccct ttatagtagt acctcatcaa
    17821 aacttattta tatatattta ttctagcata ttataataat tatttattaa ataattatta
    17881 ttaaaattta ttaagtaatt agcatttaaa tttatataat cactttaaaa tttataatta
    17941 aatttataca aatgaaatca aatgaattat acccttagta tattattttt tcaatactat
    18001 tttcaccttc aaaacatttt tctattcatc aaacaattaa tgaatgcctg tatccaatca
    18061 taaatttaag aatataatta agatagatat ttttaaacaa actatatttg catattatat
    18121 ttacataaaa aagccccaaa atgacttata aatcaatttg agacttttta ttttaaactt
    18181 aataataatc tagattaatt tctttttatt ttacattgat acgctaatcc acaaattgca
    18241 atgacactaa taaatacata tgacattagc tgagtttcat cacctgtttt tggtgatttt
    18301 attattgtat catctcttga tagttctgga gttttttcca ctgtatcact tactgatggt
    18361 actggtggat ttaacactgt atcatctgtt ggtggcactg gtggatttag cactgcatcc
    18421 tccattggtg gcactggtgg atttaacact gcatcctccg ttggtggtac tggtggattt
    18481 attattgtat catccattgg tggtactggt ggatttatta ctgtatcatc tattggtggt
    18541 gatggttttg gcttatgtcc atttgtatca tctgttggtg gcaccggtgg atttacaaca
    18601 tcatcatcta ttggtggcac tggtggattt acaacatcat catctgttgg taaacctata
    18661 ttaaagttaa cttcttgacc attctttttc tctattgtat aacttaataa attttgcttg
    18721 ctagatggta atgaagatga atggtctttt tgtcctaaat caagcactgc cttataatat
    18781 tgaatagctt ttggaaaaat ataatcataa gatacagcag aaatattagc tttggcatct
    18841 ggttttccaa cactcttaag tttaaatttc tgttgcgttg aaatttctac ttcgcctgta
    18901 cctattatat taccatctac atcataagca gttactccat ctggtagttt taactttact
    18961 acaccactat aacctactaa ggctacagct tctgtttcat aagtaccatc ttcatttttt
    19021 tcaaagttaa tcttagttaa accattactc aaactaggtt ttcctgtatc atttggcttt
    19081 aactcttcac gacctgcttt taacaatgct tctaaataag cttttgtatt tatcgctact
    19141 tctttcctct tctgatactc cgctctttcc tcttcagttt ttgctctttc tataagttta
    19201 tcacaaaggt ttatagaacc ttgatactgc ttaagtcttt cttgttcatc catttgtcca
    19261 agaataatcc aaattgcact ttgagtagct gagtaagtca tcaattcatt ttcttctcca
    19321 cctaaattaa attgttgctt atatccaaat ccatcaaatt cagaacctgc atatagtata
    19381 gatttaactg cttcttcatt ttggttttct tgaccattaa aatcttctgc tggtaaatat
    19441 ggactctttc cattactact tggtggtctt aaatgattat tgtaacaaaa tgcagtttgc
    19501 tctccatctt caccttctat tacatatttg tcgtcttttg agcttgctcc tacaagaccc
    19561 tttattacat agtcaaaagt taaagtcttt gtaatatcat caaatcttaa atcatgataa
    19621 ggtctatctg aaccatctat ttcttttagt aaacaagaat tttttacttt tgcttttttg
    19681 tcataccata agctaataaa tgagtctttt gaagtaaaaa cttggttaat ttctgattgt
    19741 tcatctgcct ttacatcttc ttcatcttcc caaaagttat atactatttt atttatttga
    19801 gataaagtca tatcaagaat tttatcaata tcttgtttta aactcatttc ctctataact
    19861 attttttctt ccaatggttt ttcttcttct ggagtttgct catctaatgg tttctccaca
    19921 tctggcatct gaccatctga tggtttctcc acatctggcg tctgactatc tgatggcttc
    19981 tctccatctg gtatctgacc atctgatggt ttctctccat ctggtatctg accatctgat
    20041 ggtttctctc catctggtat ctgaccatct gatggtttct ccccatctgg tacctgaccg
    20101 tctgatggtt tctccccatc tggtacttga ctgtctgatg gtttctcccc atctggtacc
    20161 tgaccgtctg atggtttctc tacatctggt gtctgaccat ttgattgttc ttctccatta
    20221 agtattgtat cattgctagt cacttcgtca gcataagata ctattggtgt aaatgcagta
    20281 aataccatac taaaagcaat tactaaactg attatacttg attttttaat ttttgttttc
    20341 aaaattctcc tgatactaaa aacatatatt gtattcactt aatataatta aaattattaa
    20401 atattatatg aatcttattc aataatacat ctttagtgtg ttcaattagg gtcgttactc
    20461 ctaaccagtt ctcctatgaa cttcttaata tttctattaa gcacagacta tctcattacc
    20521 taaccataaa ttaggtactc cccatttccc cacacttgta ggtacgagat ttctctctag
    20581 tcgttgaagt ttcttctttt cgaagcttac ctgctgatta tccattttca aggcacttag
    20641 gatttaacct tatgctatct aactaatttt ttctactttc gtaacctcaa gtgcttatta
    20701 aatgcacact ctatcacact tatccttatt tcatgattat gttgtggttt agttagcttt
    20761 aggaacttcc agcaattaaa ggagttttgg gcagatatct gccactctac actcgttatg
    20821 aatgcaggga gcatttcaaa aatatattta tatctatctt ctatcaatta cttatttatg
    20881 tatttatttt atatattttt gacaattaaa agttactcct atttatattt atagcaggtt
    20941 gcactactaa ctccatatat cttttaagtg cccaggttta agatatactt cacagtttct
    21001 ataaataggt ttttaatttt tcttttttaa ctttttaagt gcactttttt aacaactcaa
    21061 taaaatataa attagtatcc tagatactta tctaataaaa aacaaaaatt agtttttata
    21121 ataaaaaaga tactatagag tatcttcaaa tatcacaaaa accttttact ctatcggata
    21181 gttgacttaa tataatatgc atattaatac aaaccacata cttgagtatc attatttttg
    21241 tccaatatta attgtaatag ctattgttat cattatacaa tatctagcat attttgtcta
    21301 ttttttccat ttattttaca atatatatat tgccctcaaa atacattaaa aaacatcctt
    21361 agaagtaact tttaagtttt acttctaaag atgtctaact tctatttatc tattatttta
    21421 tttttaatct tacgtcacct tttattcttc ttattaatgg gtgattaact gttctcatta
    21481 catggtcagt aatatgatat acacctaatt ttaattttgg tgcagaaccc attactgtgt
    21541 tactacagtc agtacagttt cctattaata taacttctgc tccagctttc ttaaattcaa
    21601 tacatttttg agcttgtcct ggacagtgtc ctggattttc acattttaat gcacctttaa
    21661 tttcttgagc ttgtttacat ttttgaactg atactatatc agtaactccc attttagttg
    21721 ctatatctct cattctttct tcattaggtc cacatgcaca aactaaaaga cccatttttc
    21781 tcttttcatt aacaaatggc atagtaacta ttataccacc actagtttta gttattggag
    21841 catctaaagt agtagcttta ccagtgaaag gtcctcctag gattatttca ccatattcac
    21901 cttttattcc accagctctt tcaattaatt ctccgacagt tgttccaatt ggcacatctt
    21961 caaacactat agattctttt ccactgttta attgaccaac tacagttata tttttagata
    22021 taactggtct tttttgctca acagcctcta ctattctagt tagtgtttct acgtttatta
    22081 ctactgcatt agcctctgaa ggtaattgag ttggctcaag taattttcct agaacgtctc
    22141 ttactattgc tctctcttct cccattgggt acatatcaac taattctgca acttctatgt
    22201 tgtctcctgg ctttattact tttttgaaag cttctattgc ttttggatgc ttacttttta
    22261 ttgctagcat accttttcca gcattagtaa cttccatagc atattttata ccttgataaa
    22321 ctatttctgg tctttcttct atttctttta tattatgagc taataaaggt tcacattcag
    22381 ctgcatttgc taatattgtt ccaccattta aatctatgtt taattttaca tgagtaggga
    22441 atcctgctcc acccattcct actactccag cttctttaat taagtcaagt ataccttctc
    22501 cttttaatgg ttcaaaagta tcttcttgta cttcatcagc ttcaattact atcgcttggt
    22561 catttatttc acttattttt ccactaacac tggcataaat atttgcccct aatccagttg
    22621 gttctgcgat tagagttcct ttttttacta catctccaac acttactact ggcttatctg
    22681 gaccaccaac gtgttgtttt aataaaatag aatatagttt gttcaactag tacgccccct
    22741 taagtatatt aattttttta taagctgttc aaataatgtt aatgctattt ttctattata
    22801 aggtcttaac atattgttta tttctataga acatatcgct tattaacaaa gcaatttcta
    22861 tgccaacttt taaacaaact tatacccttt ggaaatactg tcttacagca ttcattaatt
    22921 cttctaaatt tttaaaccca tcaaaaaaaa gtcaattatt agacactttt ttaatcaatt
    22981 taatctataa ttattgatat tgcatacatg tataaaaagt gtcaaaaaat agacaccgtc
    23041 tttttatcga cacttttcta acatatacta tataatttat cacaacttta cttaaaatat
    23101 acatttatta cttattaatt cttagttttt tgcactccat tggaatcaac tacataatta
    23161 tctttataca acaaatcaga tattttgact agactgtatc catctttttt tagtcgactt
    23221 attatagtat ccaaatagtt atctacatca tttatattag catggaataa aactattgaa
    23281 ccaggttgag attcttttat tactttctca attacatgat ttggtccaag ctccttccaa
    23341 tctatagaat ctacatccca cttaattact ttataaccta aatcactaca aatgtccata
    23401 gctttattgt ttacgtcccc aaaaggaggt ctaaataaat tagtcctttt tcctgttata
    23461 ttatatattt tttctgatgt agtttctatc tcttttgtta tgtcttcttt tgatatttcc
    23521 ttcaaattgg catgagtatt tgaatgattt ccaatttcat gtcctttttc atctatagct
    23581 tttacaattt cttcattatc atccacccaa ctaccaacta aaaagaaggt agcttttaca
    23641 ttatgtttat ctaaaatttt taaaatatca tccatattat cagttcccca tgctacatca
    23701 aaactcaaag ctattttttt ctcatttgta tcaactttat atataggcat ttttgaattg
    23761 tcagatttag aacatatgta ttttattcca taaacaaaaa caaatattaa cactaataat
    23821 aatgaaaaaa ataaaacact atatatcaaa gctttagtcc ttttaacttc tcttttcata
    23881 ctattccccc tttgaaataa tttcttctat atttaaaatt tatttttttt cagctcaaaa
    23941 aagtactgta atatttaatt tataagacaa taactaaaaa aataaaacct atattgacct
    24001 aattaaactc tctaaaatac tcatttattt aattttttta tattttttac atttttacct
    24061 ttatttttct gtatatatta tttttatttt ggagaaatta gtattaacat cacataaata
    24121 aaagtgttgt tagacatcat aaactaatta tttgaggagg aaatttacta tgtcaaattc
    24181 aaacagaaca gtagttccag aagcaaaagc tgctttaaac caaatgaaat tagaaatagc
    24241 taacgaaata ggtttatcta actatgaaaa catagacaaa ggtaacttaa cagctagaga
    24301 aaatggatat gttggtggat atatgactaa gaaattagtt gaaatggctg aaagacaaat
    24361 ggctggaaaa taaatagtaa attttgacac ctgggtatca acagggctgc taattagcag
    24421 ccctgtttct ctatatttat ttataaaatt gtttttatat gaattattaa agattaataa
    24481 tacaatacaa attgttcttt taattttgta caaattagtt taatatatta tatatagaaa
    24541 tagtttgacg gaggatgata tttatgtttg aaaactcgtc tgaagaatta gcctatcaca
    24601 aacttttaat tttatatata ttagaaaaaa ttaaaatgga tttaactaat tctcaaataa
    24661 ctcaagtagt attagagact gagatgatga attatttttc attacaacag cttttatctc
    24721 agcttatgga atctaagttt ttgactacat ataaagactc tgacagagaa tattatgctc
    24781 ttactcaaag aggcgtagaa agtctagaat acttctttaa tcgaatacct actagtgtta
    24841 ctgaaaaaat agacaagtat atagattcta acaaagaaaa tctactagca gatactcaag
    24901 taaagtctag ttttgtaaaa caaagtgata atgaatttat tgtcaacctt agagtcatag
    24961 aaaatcagtc taacttaata gatttaaatc taaatgtttc atcagagaaa caagcaaaac
    25021 ttatttgcaa taactggaaa aataatgcat cttatatgta tgccgaagtt atcgatttac
    25081 ttataagaga catacattaa agcctacaaa caaattatag ttgtaggctc ttttcctacc
    25141 ttgatttctt ttattaattt gttatttgca gtttcataaa cttttactga attttcttgt
    25201 gtatctgata caaatagtaa cttttcatct ttagatattt ctaatccttg gggaatccca
    25261 tttatttcaa tatttccaat gtatctactc gttgtataat ctaaaattct tacattatta
    25321 taacccaaat cagcaacata aacttttttg ccactataat taaatcttat ttctactggt
    25381 aataaaaatt catataatac cttttttata tccatgctta cttcatctat tattataagc
    25441 tttccatcaa gtgttgatat atacatttct ttcttttgtt tatcaagttc tatatgccaa
    25501 gctctaaagt ccatatttat tttcttttgt atatttttat taattgtatc tatacatact
    25561 atcgagttta tacacggtac atataacttt ttgctttctc tatcaaagtc aaatccatgt
    25621 ggcatattat caactccaat aattcctatt ggatttaatg tgtttttatc tagtacatat
    25681 atactattag aatcttcatt tgccacaaac aattcattat cgctaagtac aacttgacta
    25741 agacttcctc ctatagagac agtatcaatt attttatcat tacttaaatc taagacatag
    25801 agtatcccat tacttgaact aggtatgtat attaaatttt tttctttttc tatgcaaaaa
    25861 tggtgtggat atatattatc ttctaatact atttcttttt ctagttcaag tgtagaataa
    25921 tcaactatgc taatactctt tgataagtag ttagaaatat agattttcat ataacccctc
    25981 ctctctacaa tacatatact acaataatat attatttcat tagcttgtat tatgctttat
    26041 agttgcatta ttacgcatat atttggtatt attattaatg ttaaaaaaag aaataatatt
    26101 taatggaggt ctttttaatg aaagtaacat ttaatccaag tggtgtatgt tgtagagaaa
    26161 tgatttttga agttgatgaa aataatgtga ttgttgatgc tgagtttata ggaggatgta
    26221 atggtaattt atcaggactt agaagcttaa taattggcca aaatgctctt gaagttgctg
    26281 ataaattaaa tggtattgac tgtggtggaa aaggtacttc ttgcccagac caattatcta
    26341 aagctatacg tcaatctata taattactta tataaacatc agtaaaatta ttaaaaactt
    26401 attttatata aaaagagttg tccatttttt agacaactct tttttatatt tttatttgta
    26461 tacttatttt ataaaattat aatttcttta ctaaagttct cactgtacca atcatatata
    26521 atgaacctgc acttattata atatcatcct cacttgatgt ttctaatgta taatttactg
    26581 catcctctat ctcatgcttt gatgttacat catctacata ttttaatact ttttctctta
    26641 atatatctga atttatggcc cttgggttat ttggtgttgt tgttattacc ttattaaaat
    26701 gtgacaacaa tatctctaaa acactatcaa tatctttatc ttctaacata cctattaata
    26761 aggttaattt tctaccttta aaattcttat caagtgcttt tgccaaagat tttgctccat
    26821 cttcattatg tgcaccatct attataaaaa ttggattttc ctttatcttt tctattcttc
    26881 ctggccattt agtatttata agtccttttc ttatagattc ttcatttata tttgctaatt
    26941 tatttatgtc ttttagatat tttattacac taattgccaa gatagaattg tttatttgat
    27001 gttcgcctat caattttatt tcaaggtctc tataagtttc tttcattaca gtacaatcgt
    27061 atatttgaga atttatatct gattttttta tgtttatatc atcaaaatca acttctatat
    27121 atttagcttt tttctcttta caaactgact ttataacatc ctttgcttca tcagtctggt
    27181 catatactag tactactcca ttttccttta ttattccagc cttttcgtac gctatttttt
    27241 ctattgtatc tccaagtata cctgtatggt ccaaacttat agatactatc acacttacaa
    27301 gtgattttgt tattatattt gtagcatcat atctgccacc taaaccaact tctaatgcca
    27361 caaagtctac cttttgttct gaatagtaat aaaatgccat tgcagtaacc acttcaaatt
    27421 ctgttggata tgcatagcct tcttttacca taatctctat tttttctttt attaactcaa
    27481 ttattctagc aacatcatcc tgtggtatgt tttgtccatt tactcttata cgctcagtaa
    27541 aagtttctaa atatggagat gtatataaac caactttata tccacactct cttagtatat
    27601 tagaaacaaa agaacacaca gaaccttttc cattagttcc agctacatgt attatattca
    27661 aagtttcttg tggatttcct aataattcta ataactttcc gatattttct aatcctaatc
    27721 ttataccgaa tttattagtt tgtgatatgt actctaatgc ttcttcatat ttcattatct
    27781 aactccccct gtattggcat tttctcttat gctatctaat tatagtatat tttttacttt
    27841 taatctaccg acaaagaata catataattt aataccttct acattaaatt tagctacaaa
    27901 tctttattta taagttttta tatctttcca ctaattcagt cagtgtatat attgaaacta
    27961 agtcatattt tgagatgtcc atataattaa actttatgtc aaatctatgc tctagctttt
    28021 tcaacagatt aaaacaatta tcataaacca agcctatctc cttattaaaa aggctaccct
    28081 taaagagttt ttcattatta tcttcaatat ctaagcaatc cctaacaacg tcagcaactt
    28141 tcataagaag agcattttct ttgtcataca aatccaattt aaagtttaca attctactac
    28201 actcatcaaa atcacaacta tcccaatgat ttctagttgc taatgattca agcttatcat
    28261 ttaaatcaga tattgttatg cctggttttt ctgataatat cttagctatt ttaccagtaa
    28321 gctttgctga taaataacta ttacattttg gtggtaatct ataggaatta tttatatcac
    28381 aacttatata tgaattgcta tccataacac attgaacatc atattttttg ttgtaccaaa
    28441 atgaatcatc tccatctaaa gtaaatcctc ttacacctat aacattgtta aacatagctg
    28501 gataacttaa gtcaaaatca ttagcgagtg aacatactat tatttttcct tttctaaaca
    28561 attcataaca aacttcaaat aagcccttaa cactttctac tcctatgact gataaactta
    28621 aattaattaa tcttatgtta gtgtctaaaa ggtatcttaa tgcttcttca agaactttta
    28681 tatttgtaat accatctttt ctcaaaattt ttattacaaa taactctaca tcttcatact
    28741 ctttctttat tattgatgca caagcagttc catgaccatc ttcatcatca aatttatctg
    28801 atataaagat ataatcatgc atacattcaa aagcaattcc tccaactaaa ttatctttta
    28861 agtaatcatg ttctttgtct attcctgtat ctaatactgc aaccttaact ttattattca
    28921 tatcacatat attctccatt acaaaagttt ttaatatatc tatgttaata gttcatctta
    28981 aactttgatt taagttgcac aatttatctt ttaaatgcta tatttcttgc taaaaacata
    29041 aaataagtac accatatatt taatttatat ttattatatc aattattatc attaaatggt
    29101 taaatgttaa gataaaattt ttagatttat atcacaattt agaattattt gtaaaaaagg
    29161 agattatctt tttattagac aatccccttg tatattttat taatatttat ctatgattaa
    29221 gcatcaattt tattttatac tttttgattt taatactata catctaaatc ttatttaggg
    29281 ggaacaatta tttatatgat ataaaaatgc taatttgaat gaacttgtct tataatcagt
    29341 tttagaaaat acaccattct acaaagtatg aatcaaaagg tgaaaaaata ctaaaaataa
    29401 taaatcaaca tcaattttaa tcaatatatg aaaaacttat accaagtatg tataaaaact
    29461 aatataaata ttattttaac aactaatact tacaccttta tatattatat catatttcaa
    29521 taaataaatt gcataaaagg aaatcttttc atagaattaa actatttatt aattcattac
    29581 tataggttca ataaactata agatgtcgct ttaata
//
LOCUS       DAENCV010000043        29063 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAENCV010000043 DAENCV010000000
VERSION     DAENCV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.043"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(43..234)
                     /locus_tag="KQD82_001876"
                     /pseudo
     CDS             complement(43..234)
                     /locus_tag="KQD82_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            504..1145
                     /locus_tag="KQD82_001877"
     CDS             504..1145
                     /locus_tag="KQD82_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG4380350.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            1463..4507
                     /locus_tag="KQD82_001878"
     CDS             1463..4507
                     /locus_tag="KQD82_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG4380351.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     gene            4740..5066
                     /locus_tag="KQD82_001879"
     CDS             4740..5066
                     /locus_tag="KQD82_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG4380352.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            5082..5465
                     /locus_tag="KQD82_001880"
     CDS             5082..5465
                     /locus_tag="KQD82_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG4380353.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            5633..6040
                     /locus_tag="KQD82_001881"
     CDS             5633..6040
                     /locus_tag="KQD82_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380354.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            6056..6382
                     /locus_tag="KQD82_001882"
     CDS             6056..6382
                     /locus_tag="KQD82_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380355.1"
                     /translation="MNLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGIGLLFLAIGVIGI
                     ICATLI"
     gene            6483..7877
                     /locus_tag="KQD82_001883"
     CDS             6483..7877
                     /locus_tag="KQD82_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4380356.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            8061..9257
                     /locus_tag="KQD82_001884"
     CDS             8061..9257
                     /locus_tag="KQD82_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG4380357.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            9270..9404
                     /locus_tag="KQD82_001885"
     CDS             9270..9404
                     /locus_tag="KQD82_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG4380358.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            9405..9626
                     /locus_tag="KQD82_001886"
     CDS             9405..9626
                     /locus_tag="KQD82_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380359.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            9830..10606
                     /locus_tag="KQD82_001887"
     CDS             9830..10606
                     /locus_tag="KQD82_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380360.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            10691..10987
                     /locus_tag="KQD82_001888"
     CDS             10691..10987
                     /locus_tag="KQD82_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4380361.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGIPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            10906..11409
                     /locus_tag="KQD82_001889"
     CDS             10906..11409
                     /locus_tag="KQD82_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG4380362.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            11427..11930
                     /locus_tag="KQD82_001890"
     CDS             11427..11930
                     /locus_tag="KQD82_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG4380363.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            12048..12446
                     /locus_tag="KQD82_001891"
     CDS             12048..12446
                     /locus_tag="KQD82_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4380364.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            12424..14874
                     /locus_tag="KQD82_001892"
     CDS             12424..14874
                     /locus_tag="KQD82_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4380365.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            14874..17069
                     /locus_tag="KQD82_001893"
     CDS             14874..17069
                     /locus_tag="KQD82_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG4380366.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            17066..18073
                     /locus_tag="KQD82_001894"
     CDS             17066..18073
                     /locus_tag="KQD82_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG4380367.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            18089..19000
                     /locus_tag="KQD82_001895"
     CDS             18089..19000
                     /locus_tag="KQD82_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG4380368.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            19121..19498
                     /locus_tag="KQD82_001896"
     CDS             19121..19498
                     /locus_tag="KQD82_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4380369.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            19499..20362
                     /locus_tag="KQD82_001897"
     CDS             19499..20362
                     /locus_tag="KQD82_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380370.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            20359..21003
                     /locus_tag="KQD82_001898"
     CDS             20359..21003
                     /locus_tag="KQD82_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG4380371.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            21016..21207
                     /locus_tag="KQD82_001899"
     CDS             21016..21207
                     /locus_tag="KQD82_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HBG4380372.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            21412..21600
                     /locus_tag="KQD82_001900"
     CDS             21412..21600
                     /locus_tag="KQD82_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG4380373.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(21708..22067)
                     /locus_tag="KQD82_001901"
     CDS             complement(21708..22067)
                     /locus_tag="KQD82_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380374.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            22254..22469
                     /locus_tag="KQD82_001902"
     CDS             22254..22469
                     /locus_tag="KQD82_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBG4380375.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            22486..22758
                     /locus_tag="KQD82_001903"
     CDS             22486..22758
                     /locus_tag="KQD82_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBG4380376.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            22760..22909
                     /locus_tag="KQD82_001904"
                     /pseudo
     CDS             22760..22909
                     /locus_tag="KQD82_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            22970..23311
                     /locus_tag="KQD82_001905"
                     /pseudo
     CDS             22970..23311
                     /locus_tag="KQD82_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            23436..24173
                     /gene="erm(B)"
                     /locus_tag="KQD82_001906"
     CDS             23436..24173
                     /gene="erm(B)"
                     /locus_tag="KQD82_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG4380377.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            24319..24603
                     /locus_tag="KQD82_001907"
     CDS             24319..24603
                     /locus_tag="KQD82_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBG4380378.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            24706..25584
                     /locus_tag="KQD82_001908"
     CDS             24706..25584
                     /locus_tag="KQD82_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG4380379.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            26032..26448
                     /locus_tag="KQD82_001909"
     CDS             26032..26448
                     /locus_tag="KQD82_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4380380.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            26455..26703
                     /locus_tag="KQD82_001910"
     CDS             26455..26703
                     /locus_tag="KQD82_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380381.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            26954..27355
                     /locus_tag="KQD82_001911"
     CDS             26954..27355
                     /locus_tag="KQD82_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380382.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            27374..28819
                     /locus_tag="KQD82_001912"
     CDS             27374..28819
                     /locus_tag="KQD82_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HBG4380383.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
BASE COUNT         9770 a         4955 c         6247 g         8091 t
ORIGIN      
        1 taactcatga ctaaagtcac gagtgtgcgt agctattttt taatcaaagt gtacaacttc
       61 tatacctttt tctattaaat tcaatgtaac tcttttatat aacttgtcta atggctctac
      121 atttggagta gtaactaaca aacatttctt tccatatttt cctattattc taggtaattc
      181 acttaaagtt ccttgtccaa aatttattct tgatggttga taaaagttaa tcatattttt
      241 cctcccttaa attattgcct tcatattcaa attatacata tcataggatt tttaaggcaa
      301 taccttcact gttaactctt atttactaaa gcttatggca attattagac aataaatgtt
      361 ttattattac catatcatta tgtaaatttt attaagtacc catgttataa ttttataatc
      421 aatcttattg tataattgac ttaataataa aaatagagta tatacacttt tttatgacaa
      481 atagcttaaa ggagaattaa attatgaaat atttgataga cagtgctaat cttgatgaaa
      541 ttcaaaagtg tttaaagtat ggatttaagg gaataacagc taatccatct atgtatctta
      601 aagagaaagt aaacttccat gaatttattg agacatgtgc taatttaaat ccagaaatat
      661 taactgctga agtcattgga aataatttat acgaattaat taattcatgt gataagatat
      721 taaatataaa taaaaacact ataataaaaa taaatttctc tgaagttggc ttagaactta
      781 taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac cttgattttc aatgtaaatc
      841 aagcatcatt agctataaat gcaggtgctg attacctatt cccttttata ggtagaaatg
      901 atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat atgtaacctt atatcaataa
      961 atggatataa tactaaagtt gttgcagctt ctattaaaaa tgtattccac ttaactcaag
     1021 catctctttg tggatgtgat tatgcagctg taacatatga tttatttaaa aaagcttctt
     1081 ttaaccagct cacaatagat ggtgccaata cttttgaaag agattggtcc aaggttaata
     1141 aatagtaact aagatttagg agttctctgc gaaccgtggt agctagaagt tcgattatcc
     1201 tcttgggagc gtttaatgcc gtaaattaaa gcataagtgc ttgttatgtt taattgttga
     1261 tactctctgg tgtaccctca tacaccgtag agtgttagca ggttgttagc aacaaaggct
     1321 aatttaaagc tttaatttat cagaaaacat tacaaacaaa ctataaatca tttatcacta
     1381 gaaatcaaga tactcgtatg ttatagacgt tcagaaatga gcgtctattt ttataccaaa
     1441 aatgaaagga gtattaaaat ttatgaaaaa gacattgaaa cgattgtgta cgggcttctt
     1501 agctcttgca actatcgtta ctgctttacc gactacaccc gttcatgcag aaagtaagca
     1561 atactggaca gaaagtgcag agcgtgtcgg tatcattgaa aaagtaatga atgacggttc
     1621 tatcggctcg acattcaatg agggcatgat gaaagttgag ggcaaaacag cctattgtat
     1681 cgacattaac acagatttta ggaatggcta caagaccaga gctgacgcaa gctcacgcat
     1741 gaatgccaac cagatttcag atgtggcgtt atccttagag tatgtcaaac agtatggcga
     1801 agctcacaag gaactgaact acaagcaagt ctatctgttg gaacaatgtg tagtctggca
     1861 gagattgagc gtacatcttg gctggcaatg cgataacgtg cgagcttctt atgatgaaat
     1921 tccaaaggca acgcaggacg aagttttctc tggtgcaaga acctttgtca aagaaaataa
     1981 aggacgctat gaatgtggcg gttatatcta ctctggcgag gggcaggaat tggggcaatt
     2041 ctgggcgaag ctgaatgttg gaaatgcgaa acttaaaaag acttccagta atgccagcat
     2101 tacagacggt aacgggaatt actcaatcgc tggtgcgacc tatggtgtct ttactgataa
     2161 ggactgcacg aaacagcttg ccacccttac gactgatgaa aacggaaata cagatgttgc
     2221 agaggtaaaa gcaggcacag tctatatcaa ggaattatcc gcaccagcag gatataaagt
     2281 agataaaact gtatattcct taaagattga agctggaaag acggcgactt tgaacgtatc
     2341 tgatacgcca aaagtaacgg acactttgat tgagcttttc aagatagata tggaaacaaa
     2401 gaaagacaat ccgcaaggga acgcttcttt agcaggtgcg gaatttacat ggaagtatta
     2461 tgctggcttc tataacaaag acaatctccc tgccgaagct actcgaacat gggttacaaa
     2521 gacaatcgct gaaacagaca gctacgggac aactcattac atcacaaaat tagcggacgc
     2581 atacaaggtg tctggcgaca gcttctatat gcaagacgga aaagccgttc ttccacttgg
     2641 gacactaacc atagaagaaa caaaagctcc aaacggctac ttgttagagg gtgcatatat
     2701 gcaggctggc gataagtccg aacagataaa gggcttatat gtaacacaga ttaccgagga
     2761 cgacgacctt gccgtattat ctggaagtaa ccagttttcc gtatcagaca aggttatccg
     2821 tggcggtgtg aaaattcaga agcgagattt agaaacgggc gatatgaagc cacaaggaag
     2881 tgcaaccttg aaagatactg cttttgacat catttcctta aatgataatt ctgttttggt
     2941 tgagggcaag ctatacaaga aaaatgaagt ggtaaagaca attcgtaccg atattgaggg
     3001 tgtcgcttct acttctgctg acctcttacc ttatggaaaa ttccgtattg ttgagagtga
     3061 agctcccgac ggttacttaa ctgacggtgc aaagccgatt gattttacaa tcacagaaaa
     3121 tggaaaaatc gtggacttaa ccgacaaagc ccattctatc tataatcaga ttaagcgtgg
     3181 agatattgag ggtgtaaaaa tcggtgcagg cacacacaag cgtcttgctg atgttccctt
     3241 taggatcaca agcaagacga caggagaaag tcatatcgtg gtaactgaca ataacgggca
     3301 gttctccact tctgctgact gggcttctca taagcacaat accaacgcag gcaagaccag
     3361 cgaggacggt gtgtggtttg gaacttctga accagacgac agcaaaggtg cgttacctta
     3421 cgatacttac gtcattgagg aattacgctc tgaaagcaac aaaggctttg aacttatccc
     3481 accttttgaa atcgtggtat caagaaataa tcttttgatt gatttaggaa cgctgactga
     3541 tgaatacgaa aaagaaatct ctatccatac cacagcgacc agcaaggacg gcgaaaagac
     3601 tatccttgca ggaaaagaga ttacgattgt tgatactgtc aaattagacg gacttacaaa
     3661 aggcacaaaa tatcagctca aaggctggca gatgttaaaa gaggaaaacg ccgagcttat
     3721 cattgacggg aaacgtgcag aaaatgatta tacctttgtc gctgatgagg aagaaatgaa
     3781 agtggaaatt gcctatacat tcaatgcgtc tgctttaggt ggcaagaacc ttgttacctt
     3841 tgaagaattg tatgatttga gcaatccaga cgaacccgtg aaagttgcgg aacacaaaga
     3901 cattgaggac gacgggcaga ccgttttaat cacagagcgt atcatcaaaa ttcatacgac
     3961 tgctacgaat aaggacggca acaaagagct tgaagctgga aaaaacgtta caatcattga
     4021 taccgtaacc ttagagggct tagaagttgg tacacagtac aaacttgtgg gctggcagat
     4081 gttgaaagaa gaaaatgcag aacttcttat caatggaaaa tgtgtggaaa atgattatac
     4141 gtttactgct gacagcgaaa ctatgaaagt ggaagttgct tttacgtttg acgctacttc
     4201 tcttgacggt aaacagcttg taacttttga agaattgtac gatttaagca atccagacga
     4261 gccgaagaaa gttaccgagc ataaggatat tgaggataag ggacagacga ttacctttaa
     4321 ggaaaagcca gaagaaccag agaaacccga aacaccacag ataccagaaa agccgagcag
     4381 accgagcgac agtcccaaaa cgggcgacag tacaaatgta atggcatttc ttgtgatgtt
     4441 gcttgcgtct gctggtggac tggctggaac atatctttac aaacgccgta aaatgaagaa
     4501 atcataaggc ggtaacggta tgatgaaaga aaaatcacgc tctccgccga agctgtcaaa
     4561 cggcagactt ttgcttattc tggcttgtct gccaccaacc tgcaagaaca cggatagtca
     4621 agggtgcgaa gcattgattt taccctttac tatctggggg attgctggta cttcccaaac
     4681 cgccagaata agaaattaca atcaatttat caaaatagaa agaaacgagg taaaacatta
     4741 tggaaatgaa atatgtcgtg ccagatatgg cacagtcttt tggaactctt gaatttgcag
     4801 gagaaagcga gcctatcttt gaaagagata aaaataaccg cagggtctta gccagacgaa
     4861 gctataacct ttattctgac gtacagaaag gcgaaaatgt tgtagtggaa attcccgtgc
     4921 aggctggcga aaagaagttc aagtatgaac agaaagtaaa acttgtcaat ccgaagttgt
     4981 acggcagagg ttacgcaatc ggggatatgg gacataccga ttatgtatta gttgctgatg
     5041 atattgtagc agttgaagaa aagtaaagga gtgaagaatt tatgagatta tcaaacgggt
     5101 ttgttattga caaagagaaa acatttggcg aattaaaatt tacagcggtg cgtgatgtgt
     5161 ttatgcagaa tgaggacggg acaccgagta cccagcttaa aaagcgtatc tatgacttga
     5221 aatgtagtct gcatggtgga attatccccg tgtccgtacc gccagaagtg ccgttaaggg
     5281 aatttccgta caatgcagtt gtagagcttg tcaatcccgt agccgatacg gtatcaagaa
     5341 agacgtttgg tggtgcagat gtggactggt atgtaaaggc agaggatatt gtgttgaaga
     5401 ataaaggcaa tcagaacgca ggcaatccac agaaccatgt accgcaggga cagacgaaaa
     5461 aataaatcta ggtcatagat tatactctga aattacagta taatgagcca aaagagccgt
     5521 atttaagcta tgtaaaataa ttttgacaag gaaaaaaagg atttgtcgaa gatattggat
     5581 agacatttga gggttaatat atgacaatga caccgaaagg agcaatcgga atatggaaat
     5641 aggtaagaaa cttaaaaatg cacgagtaca gtctggaatg acacaagaaa atgttgcaga
     5701 aaaaatcaat gtatcaaggc agacaatttc aaactgggaa aatgaaaaat cttacccaga
     5761 cattatcaat gtaatagagt tgagcaacct atattctata agtcttgacg aattgctaaa
     5821 gggtgatgaa aaaatgattg aacatttaga agaaagtaca aatgttgtaa aaagcaacca
     5881 aaaaatgata tgggcaatta ttgtaaatat cattgtcgta gcattgctga tagcattaaa
     5941 tatgtttata ccagataacc gttatttctt agttggaata ttttgtctta tggttatcac
     6001 ttcatcagca ttactttacc agataatcaa aaaattttaa ggaggatatt ttgttatgaa
     6061 tttgaacata gttttagcgg taatttgtgg agcagttgca cttgtaggag cgttttgtgt
     6121 tgtgtttcag atttatcata tgactgttat agacgcaaca gccagaggat taaagcaccc
     6181 taaattttgg ggagtattta caatgagtgg gaataattca agtggtttgc ttatgtatct
     6241 aatcggaaga agaaaatacc ctattgtaaa tatgagtgaa agcaactcaa aagaattaga
     6301 aaaacgtaaa aaatcagcag gtattggact gttatttttg gctattggtg taattggcat
     6361 tatatgtgcg accttaatat aactaaaaat gcagatttat gattatatct tgcaacattt
     6421 acaaagcagt tagtctatat gattgactgc ttttttcgta cccagaaagg agtgaatgat
     6481 ttatgcgtaa gatttggaat agaggacacc gtatcagagc cagcgacaag caccttgtct
     6541 accatttttc catagggacg cttctgtttt tattcgtggc ggttctcctg ctactgaata
     6601 tcaaacagct catgcgtacc gactgggagc atttcagctt gttagagaac ggcttgacgc
     6661 tttcccctta caacttcata accatactga tagcgactgg tgtttgtgca ttggtcgctt
     6721 ttctgtatta ccgcttctgt tacgacagtt tcaagaagct cctgcaccgt caaaagctgg
     6781 cgagaatgat actggaaaat aagtggtatg aagccgatac cgtacaagac agcggttttt
     6841 ttactgacct gcaaagcaga tcaagggaaa aaatcgtctg gtttccaaag atttattatc
     6901 aaatggaaaa gggactgctt catatccgct gtgaaattac gctgggaaaa tatcaagacc
     6961 agcttttacg gttagaggat aaattggaaa gtggattata ttgtgagctg accgacaaga
     7021 ccttacatga cggttatatc gaatataccc tgctttatga tatgatagcg aaccgcatta
     7081 ctattgatga agtacgggca gaaaatggct gtcttaaatt gatgaaaaat cttgtctggg
     7141 aatatgacgc actccctcac gctctgattg ctggtgggac tggtggcggt aaaacctatt
     7201 ttctgctgac gctcattgaa gccttactgc ataccaacgc tgtcctttac atcttagacc
     7261 cgaaaaatgc ggacttagca gacttaggga cagttatgga aaatgtgtat cacaccaaag
     7321 aaaaaatgat tgattgcgtc aatgcctttt atgagggtat ggtacagcga agtgaggaaa
     7381 tgaagcgaca cccgaactat aagacgggcg aaaactacgc ttatctggga ctgccaccct
     7441 gctttcttat ctttgatgaa tatgtggcgt tttttgaaat gctggggaca aaagaaagcg
     7501 tgggcttact tagccagtta aagaaaatcg ttatgttagg acggcaagca ggatatttcc
     7561 ttatcgttgc ctgccagcgt ccagacgcaa agtatttctc ggacggtatc agagataact
     7621 tcaatttccg cgtgggactt gggcgtatca gcgaattagg ttacggtatg ctgtttggtt
     7681 cagatgtgaa aaagcagttt ttccagaagc gtatcaaggg gcgtggctat tgtgatgtgg
     7741 gaacaagcgt tatcagcgaa ttttacacgc ctttagtccc gaaaggacat gactttttgc
     7801 agactatcgg ctctcttgca caagcaaggc aggacgggac ggcgacgtgc gaagcgaaag
     7861 gcgacggcac ggactagccg ttgctggtgt ggcgtaagcc acgccagcag gtaaaacccc
     7921 tcggtatcta acagaggggt acgtttgcaa atagacaata gacgaaaaac ataggaaaca
     7981 taaggcttct ggcatgattt cccagtaaaa atcggctgtg aagtcgcctt aaaaaacttc
     8041 acacttgaca gattgggggt atggttctga atgaagaaca atggataaaa gaattacggg
     8101 agaaacggat tgcttacggt atctcacaag gcaggctggc ggtggcttct ggtatcacaa
     8161 gggaatatct caacaagata gaaagcggaa aaatgaagcc gtcaaaggaa cttctggaaa
     8221 ctctgcataa agaactggca aggttcaatc cagaagcacc gcttaccatg ctgtttgatt
     8281 atgtgaaaat tcgttttccc acgctggata tacagcacat catcaaagat atattaaaac
     8341 tgaatatcaa ctatatgctc catgaagatt acggacatta cagttatacg gagcattatt
     8401 ctttagggga catctttatc tatacgtcgg ctgacgaaga aaaaggtgtc cttttagagt
     8461 taaaggggcg tggttgccga cagtttgaga gttacctgct ggcacagcaa agaagctggt
     8521 atgactttct catggacgca ctcatagacg gtggcgtgat gaagcgtatc gaccttgcta
     8581 tcaacgacca tacgggcatt ttggatattc cagagcttgc ggaaaaatgc agaaaacggg
     8641 aatatatcgg aaagtccaga agttataagt tttaccagtc gggcgagctt atcaagcaca
     8701 gcgaggacga cagagaatat atgggacgta ccctttatct tggttcgctg aaatctgatg
     8761 tatatttctg tatctatgaa aaggactatg agcagtatgt caagttaggg acacctctgg
     8821 aagaagccga tattatcaac cgttttgaga tacggcttag aaatgaacgt gcctattatg
     8881 cagtacggga tttgctgacg tattacgacg cagagcagac cgccttttct atcatcaatc
     8941 agtatgtacg gtttgttgat gaagaaccag acaagcggaa aaatgactgg aaactcaatg
     9001 accgctgggc ttggtttatc ggcgataatc ggcaaagtct gaaactgacg acaaagccag
     9061 aaccttatac cttagatcgt acattgcgtt gggtacaaag gcaggtagca ccgaccttga
     9121 aaatgctgaa aaagattgat aagggaaacg gtacagatta catggagaca atcgaacagc
     9181 aggcaaagct cacagaaaag catgaaatga taatcaaaca gcagacgacc cctgcaaaag
     9241 atttagtaga aatttaggag tgatgaaaaa tgtgggtatt attaaaagac ttcctgctgg
     9301 tatctatggg aatgggtatc ggcatagtct tgatgtgtat tttgaatgtc ggcaaagaag
     9361 ctgactataa aatgaaacaa ttagaagaaa gcgaggacaa ttaaatgaat ttcggacaaa
     9421 atttgtatca atggttttta agtaacgcac agagcttagt gcttatggcg attgtggtta
     9481 tcggtatcta cttagggttc aagcgtgagt tttccaaact tatcggcttc ttggtagttg
     9541 ccttgattgc tgtcggtttg gtattcaatg ctggcggtgt gaaagatgta ttgttagagc
     9601 tgttcaataa gattatcggt gcataaaatt ttattgtggt actgatatgc gaatgttata
     9661 atttgaatgt tgtattacat aaaacgctgt acttaccaga aaaatctatg aattttgtat
     9721 attatacaag gttgcaactt gaagttgcta atgtgcattt caaaaaatat agaatataac
     9781 attatggctt gataaaattt attcatcaaa tcataggagg tatcacacaa tgagtttagg
     9841 agaaaaaatc ttcaagttaa gaaaagaaaa aggaatatcg caagaagcac tagctgaaca
     9901 aataggcact actaggcagg caattagtaa atgggaaaac aatcaagggt ttccagaaac
     9961 agaaaagctt ttacaattat caaacatttt tgaagtatct acggattatc ttttgaaaga
    10021 cgaaaagaat gtaaagaata gtgaagaacg aggatattat gtaagcaagg aaatgtcggt
    10081 agggtttatc gctaatcaga aaaaattagg actttatctt gctttagggg ttatgctttg
    10141 catttttgct ggaataccat ataccttgtt tttagataat atgacttggc gttatttagg
    10201 tatggcattt tgctttgtgg caggaattat attttttgtc gtagcaattt tttatgaaaa
    10261 acaagaatac aacattttaa ggcaagaacc tttactactt gattatgaat atctaaaaga
    10321 attgaaaagc gaatacactt caaaaaagaa aaaatatgta atagttgctg ttccttgtac
    10381 tgttctcttt atagcaggac taattttctt tctatttact gtgaaaggct atatttcatg
    10441 gacagaatat cattcctttg catttttcgg tcttgctgtt ggaatgttgg gttttgtata
    10501 tacaatcaat gttatggaaa cctatgaact attagtgaaa aacgagcaat attcaacgag
    10561 tttctttttc aaactaaaaa gaaagattaa aaagagatta ggataagcta taatttaata
    10621 ttcacacaaa gcagttagtc ttatgattga ctgctttttt cataccaaaa tttcagattg
    10681 gagtgattac atgaacgata tttttaagga tatgcaggca aaagtcggct gtgaatacat
    10741 ttctgacctg ccctcttaca agcgtaaggt gtggcgtgaa atgaaacggc tgagccctgc
    10801 cgattatgaa gaaagacaat tagaagattt ttcaaagttt gtgtttggta ttccgtacca
    10861 gaccttaaaa gatgtgatga aacaacagaa aggacgtgaa gaacaatgca ggaaacaagg
    10921 gtgctggtgg aaacgagaga aacaactggc gaagaaacaa catcatactg gttcgacctg
    10981 ccgatagatg ttgccgagtt tgaagaaaag ttaggtgtcg gtgcagaaag cacggcgtac
    11041 cgcattatcg aaaaggtgtt gccttatgct gatgaagtcc acgaacatac aagcgtgtac
    11101 cagcttaacg aattagagtt tatgtaccgc cagctttcaa gcgatatgca ggaagaatat
    11161 gtatcactac ttacggtgtt tgagaattta gaagcacttt atatttgcag gaacgtgatt
    11221 acggtttatc ccgactgcaa aagcatgata gatgttgcaa ggcaaaagct gatgaatgac
    11281 tcgacgttca aacatttatc cgaggactgt caagaatact actttgactt tgaagcctac
    11341 gcttctcact tgcaggaaca cgggaaattc ttagtaacgg aacacggtat ctttgaacta
    11401 ccagagtagg aaatgaggtg gtgcttatga tagatgatat ggcggtttac attgctaatc
    11461 tcggtaaata caatgagggt tatttagtcg gtgcttggtt cacgttcccc attgacgagg
    11521 aagatgtaaa agaaaaaatc ggcttgaatg aacagtatga ggaatacgct atccatgata
    11581 ccgacaactt ccccattgcg attggcgagt atgtttccat tgaagaactc aatgagatgt
    11641 atgaaatgat agaggaactt cccgactata tcgtagagtg tctggacgaa tttatcagtc
    11701 actacgggac gctggaagaa gtcgtggaac acaaggacga tatttattat tatcccgact
    11761 gtgaaaccat gacagatgtt gcctattact acatagacga attgcaggca cttggggaca
    11821 ttccacccag cttacagaac tacattgatt atgaagccta cgggcgagat ttggatatgg
    11881 gcggttgctt tattgaaaca agccgaggga tatgcgagat accatattaa cgctggaaga
    11941 tcagcaacgg catggttgct actgacagcg tatttctctt ttaagggaca gtctgaaaat
    12001 ggctgtcctt ttctctttga agaacttacg aaaggagctg aaagaacttg aaaaagatta
    12061 aatcttatac gggtatctgg aacgtggaaa aagtcttgta tgcaatcaat gactttaacc
    12121 tgccttttcc cgttaccttt acacagatta catggtttgt gattacggaa tttatcatca
    12181 ttctgtttgg ggatattccc ccactttcca tgattgaggg agcatttctc aaatacttcg
    12241 gtattcccgt tgctctcact tggtttatgt cgcagaaaac ctttgacgga aagaagccgt
    12301 acagcttttt gaaatcacag ataacctatg ccctgcgacc taaaatcact tatgcgggaa
    12361 aagccgtaaa actgcataag cagaccttga atgaaacaat cacggcagta aggagtgtga
    12421 actatgttcc cgataaaata tattgacaat aaccttgtct ggaacaagga caatgaggtg
    12481 ttcgcttact atgagctgat accgtacaac tattcttttc tatccgcaga gcagaaattt
    12541 atcgtgcatg acagtttccg acagcttatc gcacagtccc gtgagggtaa aattcatgcg
    12601 ttgcagattg ctacggaaag ctcaatccat agtatgcagg agcagtcaaa gaagctggta
    12661 actggaaaat taaaggaagt tgcctgccag aaaatagacg aacagaccga agcgttagta
    12721 tctatgattg gtgacaatca agtggactac cgcttttttc ttggctttaa gctcatggtt
    12781 acggaagaac agctcaatct gaagaacata aaaaaatcgg cgtggctgac gttcacggaa
    12841 tttctccatg aagtgaacca cacgctgatg aatgattttg tttccatgcc gaatgatgaa
    12901 atcaaccgtt acatgaaaat ggaaaagtta ctggaaaata aaatctcccg tcgcttcaag
    12961 gtgcgtcgct tggaaatcaa tgattttggg tatctcatgg aacatcttta cggcaggaac
    13021 ggtatcgcct atgaagatta tgagtatcag ttaccaaaga agaaattgaa caaagaaacg
    13081 ctgataaaat actacgacct tatccgtccg acaaggtgtg tgattgagga aagccagcgg
    13141 tatttacgat tggaacatga ggacaaggaa agctatgtgt cctactttac cgtcaatgcg
    13201 attgtcgggg agcttgattt tccgtcaagt gaaatcttct atttccaaca acagcaattc
    13261 acattccccg ttgatacttc tatgaatgta gaaatcgtgg agaatcgaaa ggctcttacc
    13321 acagtaagaa ataagaaaaa ggaattgaaa gatttggata atcacgccta tcaagcagga
    13381 agtgaaacca gttcaaatgt ggtggacgca ttagacagcg tggatgagct ggaaacggac
    13441 ttagaccaga caaaagaaag tatgtataag ctctcttatg tagtgcgtgt atcggctgat
    13501 gatttggacg aattaaaacg ccgttgtgat gaagtcaagg acttttacga cgacctcaat
    13561 gtaaagctgg tgcgtcctgc tggggatatg ctggggcttc attctgaatt tttacctgcc
    13621 agcaagcgat atatcaatga ttatgtgcag tatgtaaaat cagatttttt agctgggctt
    13681 ggctttggtg cgacacagca gttaggagaa actacgggta tctatatggg ctattccgtt
    13741 gatacgggaa gaaatgtgta cctgcaaccg tcccttgcca gtcaaggtat aaaaggtaca
    13801 gttaccaacg cccttgcgtc tgcttttgtc ggttcgcttg gcggtggaaa gtcgttctgt
    13861 aacaatcttc tggtgtatta ttcggttctc tttgggggac aggcggttat cttagaccca
    13921 aaatcagagc gtggcaactg gaaggaaaca cttccagaaa tcgcacatga aatcaatatc
    13981 gtaaacttaa ccagcgataa aggaaatgca ggacttcttg acccattcgt gattatgaag
    14041 aatgtaaaag acgctgaaag tctggcaatc gacatcttaa cattccttac gggtatttcc
    14101 tctagggacg gcgaaaaatt ccccgtactt cgtaaagcgg tacgctccgt tacccagagc
    14161 aacagtcggg gcttgctcca tgtgatagac gagctacgcc gtgaagatac gcccatatca
    14221 agaaatatcg cagaccatat cgacagcttc acggactacg actttgcaca tctgctgttt
    14281 tcagacggga cggtagaaaa tgctatcagt ctggataacc agctcaacat cattcaagta
    14341 gctgaccttg tactgccaga taaagatacc acttttgagg aatacacgac cattgaatta
    14401 ttatcggttt ctatgctgat tgtgattagt acctttgccc ttgattttat ccattcggac
    14461 agaagtattt ttaagattgt agaccttgac gaagcgtggg cgttcttaaa tgtggcacaa
    14521 ggagaaacgc tctctaacaa gctggttcgt gctggacgag ctatgcaggc aggcgtttat
    14581 tttgttaccc agtctgccta tgatgtttca aaggaaagtc tgaaaaacaa tatcggcttg
    14641 aagtttgcct ttcgttctac tgacattaac gagataaaac agaccttaga gttttttggt
    14701 atcgacaagg acgacgagaa caaccagaaa cgcttgcgtg atttagagaa cggacaatgc
    14761 ttattgcagg acttatacgg acgtgtcggt gtggtgcaga tacaccccgt ctttgaagaa
    14821 ctgctacacg cctttgatac cagaccgccc gtacagagaa atgaggtgga gtgatgaaag
    14881 aaaggataaa aggtgcgttc acaaaaaaga agattttcca ctttctcaaa atggttctgt
    14941 tcgtggtggc actctccctt atcctgcttt cacttctggg gacggtggct catgcgacgg
    15001 ggcttgtgga cgataccata aatgcagaaa atctttactc aaaatatccg ctgtcgaatt
    15061 accagcttga tttttatgtg gataatagct ggtcgtggct tccgtggaac tggctggacg
    15121 gtatcggaaa atcggtgcag tatgggcttt actgcattac caactttgtc tggacgataa
    15181 gcctttattt aagcaatgcc acgggctatg tggtgcagga agcctataaa ctggacttca
    15241 ttaacgatat ggcagacagt atcggaaaaa gcatacagac cattgcaggc gtaacacaga
    15301 acggtttttc ttctacgggc ttctatgttg gtttcctgct tctcattatc ttagtggtgg
    15361 gactttatgt tgcctatacg ggactgataa aacgggaaac cagcaaggca cttcatgccg
    15421 ttattaactt tgtggtggtg ttcgtgctgt ccgcttcgtt tattgcctac gctcccgact
    15481 acatcaaaaa gataaatgaa ttttcaagtg atattagtac cgcttcgttg gacttgggaa
    15541 caaaaatcat gctcccaaac tctgacagca agggcaagga cagcgtggac ttgataaggg
    15601 acagcctatt ttctattcaa gtggaacagc cgtggttact cttacaattc gggaacagca
    15661 acgcagaaga aatcggggca gaccgtgtag aagctctggt atcggcaagc ccagaggacg
    15721 aggacggaaa gaccagagag gaagtcgtga aaacagaaat cgaggacaac gacaacaaca
    15781 atctgacgat accgcaggtc gtgaaccgtt taggtatggt gttcttccta ctgttcttca
    15841 acttagggat aacgatattt gtattcttgc ttacgggcat gatgttattc agccagatac
    15901 tttttattat ctttgctatg tttctgccta tcagcttttt actttccatg ataccgagct
    15961 atgaaagcat ggcaaagcag gcaatcgtga gggtgtttaa taccataatg acacgagcag
    16021 gaataacgct cattgtaacg gtggcgttca gcatttccag tatgttttat aacatatcca
    16081 cggactatcc gtttttcatg gtggcgttct tacagatagt gtgtttcgct ggtatctaca
    16141 tgaagctggg tgacttaatg agtatgttct ctttgaacgc taatgatagt caaagcatgg
    16201 gacgaagaat tttccgcaga ccatatctat acttggcaca tagggctaga cgtatggaaa
    16261 gacggcttgc tggtgcggtt actgctggtg gtgttgctgg tgcggtggct ggtagcacga
    16321 tttcaaacag aaaagcgaca aggaacacag ctacaaaaga tagtcggggt aatacatctt
    16381 ccagcatggg acagcgtgta ggttcaaagg tgggtgctgt cttagataca aaaaataaag
    16441 tgaaagataa ggcaagtgct gtcaaagaga atatcaagga tatgccgaca cagaccgctt
    16501 atgccgtcca ctccgcaaag gaaaaggcaa agtccagcgt gtccgacttc aagcgtggca
    16561 tggtgcagga acagcagtcc agacagtcgg gacgtatgga aaaacgggaa cagcataggc
    16621 agaatatcgc cgataagcgt atggagcttc aaaaggcaca agaagcaagg caggcacagc
    16681 gaaaggctga cggatcagcg acaacgggag ctacccgtcc ccatgagcga ccagccacag
    16741 cttcaaccat tccaaagccg agtgctgaaa aaacgcagga agtcaaacgc cctgccacag
    16801 cgacgacttc aaaaacaaat gagccagtca agacaacggt tatcaaagag cgtcccttat
    16861 cttcgggtac ttctgatagg aaaacgagcc agcctacaca gccagtacat aggcagaatg
    16921 tagaaaaagt ggtatcgcag gaaacacgcc agaatgacac caaagaccgc aggacaaaag
    16981 ttcagcagac gcaaagcgtc cagaaaaacc agcagaccac agagaaaacc cgtaaccttg
    17041 taatgaagaa aggacagaag aaaaaatgaa actgaaacat attgctatta tcggcagtct
    17101 gtttcctatc ctcttttcac tggtgctttt ctttggggtg ttgattagtg cggacagcga
    17161 cgacgagaac agcaactttt cttctggcat tacgggtatg aacttatccg cagaagtctt
    17221 gaaacatcag cctatggtgg aaaagtacgc cagagaatac ggcatttccg agtatgtcaa
    17281 tgtgttgctg gcaatcatac aagtggaaag tggcggtaca gccgaggacg ttatgcaaag
    17341 ctctgaaagt ctgggactac cgcccaattc attagataca gaaagttcaa tcaagcaggg
    17401 gtgtaaatat tttgcgtccc tgctttcgtc ctgcaaaaat caaggtatgg acgatttgaa
    17461 tgtagcgata cagtcttata actatggcgg tggttatgtg ggatatgtgg caggaaaagg
    17521 aaataaacac actttcaacc ttgcagagag ctttgcccgt gaaaaatcgg gtggaaagaa
    17581 agtaacatac accaatccaa tagctgttgc gaagaatgga ggctggcggt atagctatgg
    17641 aaatatgttc tatgtggaat tagtcaatca gtatttaact gttccgcagg tgtcgggaga
    17701 gctggcacaa aaggtaatga atgaagcgtt gaagtatcaa ggctggaagt acgtctatgg
    17761 tggtagtaat cccaacactt cctttgactg ttcgggactg acacaatggt gttatggaaa
    17821 agctggtatc tccttaccga gaacggcaca agcacagtat gatgctaccc aacatctccc
    17881 actctcgcag gcaaaggctg gggacttggt atttttccat tctacctata acgctggttc
    17941 gtatgtaacc cacgtcggca tttatgtagg aaataaccag atgtaccatg caggcgaccc
    18001 gataggattt gcagacctaa gcagtagtta ctggcaacag cacttaatcg gtgcaggacg
    18061 agtaaaacaa tagaaaggac ttgaagatat gtttaagaaa aataagaaac agacagaaac
    18121 tatcaaagaa ccaaaagaaa aaaaggtgcg tactgtcaag gtaggcacac ataagaaaac
    18181 tgtgattgcc ttgtgggtgg tgcttatcgc aagcgtgagc ttcggggtgt ataagaattt
    18241 taccgctatc gaccagcaca ccacccatga aaaagaaatc attgagcttc gcttgcagga
    18301 caccaacggg atagaaaatt tcgtgaagaa ctttgcgaag tcatattaca catggaataa
    18361 cagcaaagaa gcgattgaag caaggacgca ggcaatcagc ggttatctga caaaggaatt
    18421 gcaggacttg aatatagata ccattagaac cgacataccg accagctcca cagttacaga
    18481 tgtgattgta tggcatatcg agcaatcggg aacggatact ttttctgcta cctacgaagt
    18541 agatcagcag ataaaagagg gcgaacagac aagcaatgtg aaagcaacct atactgtaaa
    18601 agttcatgta gacgctgacg gggatatggt aatcgttcag aaccctactc ttgcaccagc
    18661 aatcgaaaaa tcagactatg atccaaagac accagaaaca gacgcaagtg tagacgctga
    18721 tactgcaaat gacgctaccg catttctgga aacattcttt aagttgtacc caacagccac
    18781 agaaaacgaa cttgcttatt atgtgtcggg gaatgtgctt gaacctatcg gcagggacta
    18841 cctttattct gaactggtaa accctatctt tacaaaagac ggggagaatg taaaagttaa
    18901 ggttgctgta aaattccttg ataatcagac aaaggcaacg caagtatcgc agtatgagct
    18961 tgtgctacat aaggatagta actggaagat tgtaggataa ttgtaaaagg cttgtatcat
    19021 atttttatgc tctgcaagcc ttttaatatt gacaactgta tttttaagta ttatgctgta
    19081 cttgtataag ctatacagta tagaacaagg cggtgtttaa atggaaatct ttatcaataa
    19141 tcatgcaagc atacctattt atgaacaaat ttcttcgcaa ataaaagcat tgattataaa
    19201 tggggaatta aaggctggcg aagctctacc atcaataaga ggacttgcaa aatcattgca
    19261 tataagtgta ttgactgttc aaaaatctta tgatgtgctt caaaaagacg gttttatcga
    19321 aacaacagca ggcaaagggt gttttgtatc tgctcaaaat caagattttt atatagaaga
    19381 acaacagaaa aaaatcgaag atagatttgt agaagctatt gaaatagccc gtgcaagtgg
    19441 cataaacctt gataaattga ttggattatt aaaattatta tatgaggagg acgagtaaat
    19501 ggctgaaaat attttagaag ttaaaaacct tacaaaagat tatggcgatt ttgtattaaa
    19561 tgacttaagt ttttccattc ctagaggtgt aattatgggg cttattggcg aaaatggagc
    19621 tggaaaatcg accacagtaa attgtatttt gaacgaaatt aagaaaaaca gtggaaacat
    19681 tttgattttt ggaaaagacc acataattga tgaatgtgaa ataaaagata aaatcggaat
    19741 tgtatttgac gaaaaccact ttccagatat ttttacacca aaagaaattg aaaaatatat
    19801 gtcgggaata tataccaatt gggacagtat agcatttcgt aattatttga aacagttcga
    19861 attaccagca aagaagaaaa ttaaagattt ttctaaagga atgaaagtaa aacttgcatt
    19921 cgctgttgct ctttcacata aagcagaatt acttatatta gatgaagcaa caagtggatt
    19981 ggaccctatt attcgagatg atattcttga tatactgatt gattttgtgc aagatgaaaa
    20041 ttactctatt ttgttttcat cacatattac aagtgattta gaaaaaatag cagattatat
    20101 tacttttatt cataaaggac ggttagtttt ctgctcatta aaagacgact taattgataa
    20161 ctatggtgtt gtaagttgtg gagcaagtgt ctttgatacg ctagataaga cagaaattat
    20221 ttcctatcaa aaagaagatt atcaatataa ggttcttgtt cgtgatagaa acaagattgc
    20281 aaaagatttt ccaaaagcac ttgtagaacc tgctactatt gaaaatatta tgttgtttta
    20341 cgtaaagggg gaattacaat gaaaggctta ttgatgaaag atttattagt aattcagaaa
    20401 aaatatggaa ttactagaat tattatggat atattcatta tagtagcttt gatgttattt
    20461 ttaaaaggga caggagctat ttacattagt ttattgttaa ttccagttga aataatgaca
    20521 atgataattt ccttaacgac ttgcgacgaa caatggaaat gggggaaata tgttgttgca
    20581 ctacccgttt caaaaactac gattgtaaaa agcagatata tagttgcttt gattatgact
    20641 tgtattggct ttggggtagc tttgcttgtt aataccataa catattttat attccctcaa
    20701 tttgcatttg gttattatat gtttattgct tgtgcttctt ttgccgtaac cttagtattt
    20761 ttgtcatttg tattgccttc aaattattca cttggagtta atgcaggttt tgcggttatg
    20821 tttactctag tgattattct tgttgtattg gcagtttgga caaatgtgac taacaattct
    20881 attttatggt ttgtagtcga taattttgaa ttgagtatcg gcatagcttt tataatagtt
    20941 atagctcttg ctttcagttc ttatcttcta tcaaaagcat tttttactag aaaatacaat
    21001 tagtgaggta ttgttatgag aaaatgtctt agatgtcatg aagaaatggt cgaaaattta
    21061 gatgttaaag ttgatatgca gggatatggt attagaataa ctactaaagg cgtttttgga
    21121 actacggtag aaaaacctaa agttgcagtt tgtccaaaat gtggcgaagt ttccttatat
    21181 attgaaaatt ttaaatcaat aaaataaatg attaaaatct gcatatagac tgagggttta
    21241 ttgtttgcta acgtccgagc ggatattgaa gaatttatga atagtacata cccagagatg
    21301 caagcaaatt tcaaaaggta ttttggaaat agacatttta cgccgaaaaa atatgtttat
    21361 atcactacga aaaagcaaaa attaaatttc ttaaaagaat aagggggggc tatggataag
    21421 caattatggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
    21481 acagaactga taaattttcc tttgttctgt ccgaagtgca aaacggaaac tttaatcaat
    21541 ataaagcatg gaaaagtaac tattatcaaa gagccagacg caaagacgca gagccgataa
    21601 tttgtaggtt attatgactt atagattgtt ggctcttatt tgttacctgc ggagcagatc
    21661 agcaaataga taatgcagaa tagcaaaaag ggaaaacaag aagctgatta cttcttgctc
    21721 tccctgctgt tcaagatacc gtcaatcgta ccttgaatga tttttaattc gttatcactc
    21781 aataaatcaa gagaggtttc tatctgacgg cgagctgtac tttttacgac gttctctgct
    21841 ggatagaaat attcatcgac cgatatattg aacatggtaa ctaaatcatg gaataattga
    21901 aaactagggt gtgaaccaac attttcaata tctgcgatat ggcgttctcc atagaacact
    21961 ttatcgccta aatcattacg agaaagccct gctctctcac gggcttctct gattgccagt
    22021 cctaaaggtc tgaaatcaaa agtttgtgtt tcttttgctt ttctcatttt aaatcaccta
    22081 gcatgagttt atacttcatg ctgtatattt agaatgtgtt aaaattgagc gaatcattca
    22141 attagaaaac gaaaaagaat agaatcacaa atcacaagtg attaatcaca aatcacttgt
    22201 gatttgtgat tgttgatgat aaaataagag taagaagaaa tagaaagaag tgagtggttg
    22261 tgggaaattt aggcgcacaa aaagcaaaac gaaatgatac accaatcagt gcaaaaaaag
    22321 atattatggg ggataagacg gttcgtgttc gtgctgactt gcaccatatt ataaaaatcg
    22381 aaacagcaaa gaatggcgga aacgtaaaag aagttatgga taaagcctta gaagaatata
    22441 ttcggaaata tttacctgac aaactttaaa aggagtgaaa atgaaatggc agttacttat
    22501 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
    22561 ttaaactatg ttttgaatca tgaattaaat aaaaatgact ctcaattatt ggaagtcaat
    22621 ttattaaacc aattaaagct tgcaaaacgt gtaaatcttt ttgattattc tttagaagaa
    22681 ttacaagccg ttcatgagta ttggcggtca atgaatcgct attcaaaaca agttttgaat
    22741 aaagagaaag tggcttaata tggcaaatat agtcaatttt actgacaaac aatttgaaaa
    22801 tcgcttaaat gataatttag aagaattggt tcaaggaaaa aaagcggttg aatcgccaac
    22861 cgctttttta cttggtgggc aaccagggtc agggaaaacg agtttgcgaa gaagatgatt
    22921 gagcaggcag gaacaaagtg ataaattcaa attgtgaggg aaaatataga tgaatcaagg
    22981 aagaattatt gtgattacag gctcaccggg gacaggaaaa acaacaactg cgtcaattgt
    23041 cgcaaaggaa tcagatatgg ataaatcagt gcatatgcac accgatgact tttttcatta
    23101 cttaagcaaa ggcgcaatac caccacattt gccagaatcc aacaagcaaa atctggttgt
    23161 cattgaagcc tttttagagg ctgcaaagcg atatgctcgt ggcggatatg atgtaattgt
    23221 agatgggatt gtcgggccat ggtttttgga gccatggaaa gctcttgccc aagaagatta
    23281 cgaggtacac tatattgtat taagagcgag tatggaaata agacttagaa gcaaacttaa
    23341 gagtgtgttg atagtgcagt atcttaaaat tttgtataat aggaattgaa gttaaattag
    23401 atgctaaaaa tttgtaatta agaaggagtg attacatgaa caaaaatata aaatattctc
    23461 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
    23521 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
    23581 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
    23641 aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat
    23701 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
    23761 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
    23821 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
    23881 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
    23941 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
    24001 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
    24061 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
    24121 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
    24181 agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta
    24241 ggtatactac tgacagcttc caaggagcta aagaggtccc tagcgcctac ggggaatttg
    24301 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
    24361 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
    24421 tgtaccttca ctttagctga acattttaga aagaaaaaac tcaccaaaac aaatgtaaaa
    24481 gaattactag agggaaaaga aaccctggta aaaggaatca aaacgaaaga tagaaagtcc
    24541 tacaatgccg ttgtaaaaat cggagaaaag ggatatattg attttatctc tttttcaaaa
    24601 taagcataaa agccctttaa agagggcttt tatatattaa tcacaaatca cttatcacaa
    24661 atcacaagtg attaatcaca aatcacttgt gatttgtgat tcctaatgat acaatattac
    24721 tatacaaaaa aagaatgggg cgtagttatg gagaaggaag aactcaaaat acttgaagaa
    24781 ttaagacgta ttttaaacag taaaaatgaa gcgattgtta tcttaaacaa ttattttaaa
    24841 ggtggtgttg gaaagtcaaa attatcgact atgtttgctt acttgacaga caaatttaat
    24901 ttaaaagttt taatgatcga taaggactta caagcaacat tgacaaaaga cttagcaaaa
    24961 acatttaagg tagaattgcc acgtgttaat ttttatgaag gactgaaaaa tggaaacttg
    25021 gcttcttcta ttgttcattt gactgataat ttagacttga tccctggcac gtttgatttg
    25081 atgttactgc caaaattaac tcgctcatgg acttttgaaa atgaaagtag attgcttgct
    25141 actcttttag cacctttaaa aagtgactat gatctcatta ttattgatac tgtaccaacg
    25201 ccaagcgttt atacaaataa tgcaatcgtg gcaagtgatt acgtcatgat ccctttacaa
    25261 gcagaagaag aaagtacaaa caacattcaa aactatattt cctatttgat tgatttacaa
    25321 gaacagttta accctggact agatatgatc ggttttgttc cttatttagt tgatacggac
    25381 agcacaacga taaaatcaaa cctggaagaa ctgtacaagc aacataaaga agataacttg
    25441 gttttccaaa atattatcaa gcgaagtaat aaagtaagta cctggtctaa aaacggcatt
    25501 acagaacaca aaggctatga caaaaaagtt ttatctatgt atgagaacgt attttttgaa
    25561 atgcttgcat atagtatgca gtaataattc aaaagctgtt atcgtgtgcg gtaaataaaa
    25621 aaaaccgttc ctagataata cacttttgtg cgtcttgaaa aataacggga cggttttgga
    25681 tataaccaga actgtccttt tttcttagga gtaaataagg catagctctg gttaaagggg
    25741 gctataccat gattgcatac ggaagaacgt tctgccgttc tcaactgaat aaatatttca
    25801 gtccactatt acaatcatta caccctttac aagaattaaa aattcatatc aatagaattt
    25861 ataaaacagt ctgtttgtgt aattacacat tatcgggctg tttttgtttt gttaaaaatt
    25921 tttttcaact tttttcggtt tcaacttagc aaatcacacc tgtcgttccc tatatggtgc
    25981 ggaaagaggg ataaccactt ttcatatcgt aacggaaagg gggtgagatt gatgaaacca
    26041 tctgacttcc agaaaacagt tcaatgccgt tttgaaagtt gtttgaagaa agttgtccga
    26101 catgttgtta aggactatca gaagaaatta aagcgacgac aaaagggaga aacgcttttt
    26161 tgtgaacttc cagaaatcgt tgtagaaaat ctggctgtct gggacgacta tgatacggac
    26221 tatacgattt tcaatgtatg tggtagtgat attcgtattt gtgatgatga actggcagaa
    26281 gccttgaaac atttgtctga gcgtaatcga gaaaatttgt tgatgtatta tttcttggaa
    26341 atgagcgata ccgagattgc aaaaagacaa aatatttcaa gaagtggtgt ttttcaaaac
    26401 cgacacaatt cactagagct tatgaaaaaa atactaaagg agaaatgata gcaaatgaaa
    26461 cacacaatga agaagccgtc ctactacttg ctttcgcagg caatggacgg ggacgaaaaa
    26521 gcgattgaaa agatactggc attttatgac ccttacatat caaagtgctg tctacgacca
    26581 ctctatgatg aatacggcaa tgtctatatt gttgtagata tggaattaaa gggacttatc
    26641 agagaagcac ttattaaaat gattttaggg tttgatattg ccttagaaat cgaagaagaa
    26701 taacaagcag taaccgcaga gcatgatttg ttcaatcccc tctttcctgc tctgcccctg
    26761 cttttatatg aaagcaacac gctttcgcca cagctctttg acaactgaat aaagcagaca
    26821 gatacgtttg tgagatagcg agccacggga actgtacgcc acgacctttt caaaagaaag
    26881 cgagcgaccc acgcagtgat ccgagagcga ctgttggaaa agtcgttttg ccatgaccta
    26941 tcctcacaga ataatgatac gtccgcatgg tgcggttctg cccacaagaa tgggaatagt
    27001 tgagatacta acggagcttt ccaaagccgt ctgcctgctt ataattgata gtttcagcaa
    27061 caaaatctat ctactctttt tatttaggtg tgttataatg accttattag acgagtgtat
    27121 tttgttgctg tcacaaatga aaggggtctt aatagtgata agtgatacac aaaatgcaca
    27181 atctcctaca aaggaaaaaa agacttatac agtagaggaa attgctgaac aactggacat
    27241 tagcaaaaag gtagcttatt cattagtaaa aagcggtcaa ttcagttatg ttcgtgctgg
    27301 taaagccata cgggtatcaa aggtttcttt cgataaatgg ttaaatgaaa tttaactata
    27361 aggagagaaa attatggctt caatcaaaca aagaaaatca agtttttcag ttatttattg
    27421 gtacttagat agtgcaggag aaagaaaaca aaaatgggac acgctggaaa caagaaaaga
    27481 agcaaaacag cgaaaagcct ttatagaata ctatcaagag aaatttggat atgtgatagt
    27541 tccattagag gaacaatttg cacatcagat tgaaaattct aaaaaggagt tagatgttgt
    27601 agacaaagat attacattgc gtgatttcct agaaattttt gttaatttat atggcacatc
    27661 taaatggtcg ccgagtacat ttagtagtaa ggtaggcagt attgaaaatt atatcaatcc
    27721 gattattgga gattggaaac tgaatgaaat aactacaaaa agtctatcaa aatactacaa
    27781 tgatttattg agcgttccag aagtaccacg ggcaaacaga aaagcaactg gtcgttgtgt
    27841 tcaaccagcg aacatcaaaa aaatacatga tattatccgc tgtgccttaa atcaagctat
    27901 ccgctgggag tatattgata caaacaaaag aaaccctgcg tctttggcta cgttaccaaa
    27961 aattccaaag actaggcgaa aagtgtggag tgttcaaaca tttcgagaag cggtaaaagc
    28021 cacggaagat gatttgcttt caatttgtat gcacttagct ttttcctgct ctatgcgaat
    28081 tggagaaatt acaggactta catgggaaga tgttattatt gatgaagagt ccattgctac
    28141 aaataatgca aaggtaatca tcaataaaga actttcaaga gtaaatgcag aagctatgca
    28201 gaaattaaag gaaaaggata ttttaaagat tttcccaacg caaaaaccgc attgtaccac
    28261 aaggcttgtc ttgaaaacgc caaaaacaga aacaagtaat cgtgttgtct ggttacctaa
    28321 aacagttgcg gaactattgg tgcaatataa aaaagaccag caggaattaa aggaattttt
    28381 aggaagtgcc tacaatgatt acaaccttgt tattgcgttg gataatggca atcccgtgga
    28441 aagccgtatt gttagagata ggtttcaaaa gctctgtgaa gaaaacgatt atgaagttgt
    28501 ggtatttcac agcctacgcc atttaagtac gggttataaa ctcaaaatga caaacggaga
    28561 tgttaagtcc gtacaaggcg atactggtca cgcagaagca gaaatggtta cagatgtata
    28621 ttcagaaatc attgacgagg acagacgttt caatgcacaa aaaatggata aagaatttta
    28681 ttctacgctt aatgatgata cagacaatcc aaagcaagat accgacttaa ccgatagtga
    28741 tatggcgtta ttggaactga ttaaatcttt atcgccagaa atgaaagagc aatttttaaa
    28801 gcaggcactt caaagataga atttcaaaag tgttagcaaa ttccaaaaaa accagcgatt
    28861 tttcttcaaa aataaaaaag tgttagcaag cctatttcct taaaagtctt aaaaaaatat
    28921 cgaataaaaa atgacagatt gaaaacaatt cgtattcagc gtaaatagaa agccataaat
    28981 aaagaaacgc tgaaaacctt gtaaaataag gctttcagcg gagtttgtgg cgtcccagac
    29041 aggagtcgaa cctgcgacgt ttc
//
LOCUS       DAENCV010000044        28752 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAENCV010000044 DAENCV010000000
VERSION     DAENCV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.044"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..197
                     /locus_tag="KQD82_001913"
     CDS             <1..197
                     /locus_tag="KQD82_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4380384.1"
                     /translation="IVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRD
                     INASINILKEGLRLITIQNK"
     gene            315..620
                     /locus_tag="KQD82_001914"
                     /pseudo
     CDS             315..620
                     /locus_tag="KQD82_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            633..1538
                     /gene="truB"
                     /locus_tag="KQD82_001915"
     CDS             633..1538
                     /gene="truB"
                     /locus_tag="KQD82_001915"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG4380385.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYCNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1669..2598
                     /locus_tag="KQD82_001916"
     CDS             1669..2598
                     /locus_tag="KQD82_001916"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG4380386.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2702..2959
                     /gene="rpsO"
                     /locus_tag="KQD82_001917"
     CDS             2702..2959
                     /gene="rpsO"
                     /locus_tag="KQD82_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG4380387.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3199..4044
                     /locus_tag="KQD82_001918"
     CDS             3199..4044
                     /locus_tag="KQD82_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG4380388.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4169..6280
                     /gene="pnp"
                     /locus_tag="KQD82_001919"
     CDS             4169..6280
                     /gene="pnp"
                     /locus_tag="KQD82_001919"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG4380389.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6546..7289
                     /locus_tag="KQD82_001920"
     CDS             6546..7289
                     /locus_tag="KQD82_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4380390.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7447..8694
                     /locus_tag="KQD82_001921"
     CDS             7447..8694
                     /locus_tag="KQD82_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG4380391.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8729..8974
                     /locus_tag="KQD82_001922"
     CDS             8729..8974
                     /locus_tag="KQD82_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG4380392.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            8984..10207
                     /gene="dapG"
                     /locus_tag="KQD82_001923"
     CDS             8984..10207
                     /gene="dapG"
                     /locus_tag="KQD82_001923"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG4380393.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10320..11054
                     /locus_tag="KQD82_001924"
     CDS             10320..11054
                     /locus_tag="KQD82_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG4380394.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11177..13588
                     /locus_tag="KQD82_001925"
     CDS             11177..13588
                     /locus_tag="KQD82_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG4380395.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13591..14649
                     /gene="mnmH"
                     /locus_tag="KQD82_001926"
     CDS             13591..14649
                     /gene="mnmH"
                     /locus_tag="KQD82_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG4380396.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14643..15977
                     /gene="rimO"
                     /locus_tag="KQD82_001927"
     CDS             14643..15977
                     /gene="rimO"
                     /locus_tag="KQD82_001927"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG4380397.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            15964..16506
                     /gene="pgsA"
                     /locus_tag="KQD82_001928"
     CDS             15964..16506
                     /gene="pgsA"
                     /locus_tag="KQD82_001928"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG4380398.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16794..17840
                     /gene="recA"
                     /locus_tag="KQD82_001929"
     CDS             16794..17840
                     /gene="recA"
                     /locus_tag="KQD82_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG4380399.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18016..19557
                     /gene="rny"
                     /locus_tag="KQD82_001930"
     CDS             18016..19557
                     /gene="rny"
                     /locus_tag="KQD82_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG4380400.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            20192..21973
                     /locus_tag="KQD82_001931"
     CDS             20192..21973
                     /locus_tag="KQD82_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG4380401.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(22055..22585)
                     /gene="lepB"
                     /locus_tag="KQD82_001932"
     CDS             complement(22055..22585)
                     /gene="lepB"
                     /locus_tag="KQD82_001932"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG4380402.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(22625..23449)
                     /locus_tag="KQD82_001933"
     CDS             complement(22625..23449)
                     /locus_tag="KQD82_001933"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG4380403.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            23706..24614
                     /locus_tag="KQD82_001934"
     CDS             23706..24614
                     /locus_tag="KQD82_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4380404.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            24758..26203
                     /locus_tag="KQD82_001935"
     CDS             24758..26203
                     /locus_tag="KQD82_001935"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG4380405.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWTQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIINDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            26515..26967
                     /locus_tag="KQD82_001936"
     CDS             26515..26967
                     /locus_tag="KQD82_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380406.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(27302..28498)
                     /locus_tag="KQD82_001937"
     CDS             complement(27302..28498)
                     /locus_tag="KQD82_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4380407.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT        11650 a         3125 c         5119 g         8858 t
ORIGIN      
        1 aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa tgtgggtata
       61 aaaacgagga agttaaggat ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa
      121 ctcacgatag agatataaat gcaagtataa atatattaaa agaaggatta agactaataa
      181 cgattcaaaa taaataactt atgtaagaac cgtaggaact acggggatag cctgtggaga
      241 attagtaaga catattttaa tatgcaaaat tctgtgaagc aggaaccctg tgactttagt
      301 catgggaggt tcagtcaaag gtatctagta tagttttaac tcaagacatg atgaatagaa
      361 ataatattag ctttaatgat gttgatggaa taacaacata tacaagagac ataaaaggta
      421 tagaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagtg agttttaggt
      481 caaaaaatta tgttgatgta agtgaaatag ccaagttatt tggcggtggt ggtcatgtaa
      541 gggctgctgg atgtactatt agagatagta ttgataatgc aaagaaaatg gtactagagg
      601 cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt atattgaaac
      661 ccacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta aatataaaaa
      721 aagttggtca tacaggtact cttgacccag atgcttcagg tgttttacca atctgtatag
      781 ggaaagctac aaaagtatgt gaagttattt taaacaaaga taaatcgtat atatgtgagc
      841 ttactttagg tataagtaca gatacttatg atgcttcagg tgaaatttta aaaaaagttg
      901 atgattttaa atttagtaat gaagacatag aaagagcttt tgacacacaa agaggagaga
      961 taaatcaact tcctccaatt tattcagctt taaaggtaaa tggaaagaga atgtgtgatt
     1021 tagtaagaag tggtagacaa tctgaaatta ctttaaaaac aagaagagta aatataaaag
     1081 acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag tgttctaaag
     1141 gtacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt ggagctcata
     1201 tgtcgttttt gaatagaaca tcttctggta aatttgactt agataattca ataacattag
     1261 aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat gatatagatt
     1321 atgtccttga ttcgtttaat tatgttgtat taaaccctaa tgctataaaa tattattgta
     1381 atggtggctc aatagatgat aaaaggtttt taaaaaataa tttcgataaa gatgatgaat
     1441 ttgtaagagt atacagtaca gataatttct taggattagg aaagttatca aaacacaata
     1501 atacaataag tgttaaaagt gataagatgt tcatataacc aatgtattta aaaaaacaac
     1561 aaaagagata gtactataat aacttctata gtctatctct tttacttatg ctataatagt
     1621 ttgtgaataa gttaagagta aattatattt tagcggggaa catttattat ggtgattata
     1681 aaatctataa aagaaatagc aaacataaaa gaaagtgtca ttaccatagg aaattttgat
     1741 ggtgtacaca aaggacatca agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa
     1801 aatatacaga gtattgtatt tacatttgca aatcatcctg taaattactt tagaccaaac
     1861 tcaacaaaaa atattataag caatgatgat aaaataaaat tattagaaga tttgggaata
     1921 gatatagttg taatcatacc atttgatgag tatatgacta aaatacctgc aaaggatttc
     1981 gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga tagtaattgg acatgacttt
     2041 acatttgcaa gggcaaaaga aggcaatgtt aatctattaa aaagcttaga acatgaattt
     2101 ggttttgagg tagaagtaat aaaacccatc aaaatcaatg atgtaagagt gagtagtact
     2161 tatattagaa gtttagtatc ccagggtgat atggctaatg tcaaggaata tttgggtaga
     2221 aattacaagt tagaaggttg tgttatacat tctaaacacc ttggaagaac tataggattc
     2281 ccaacggcaa atattgacct taagaataat atgctcgttc caaagagagg tatttatgct
     2341 tctattgtac atattggaga tgagacttat tttggtgcta caaatgtagg ctataaccca
     2401 acagttaatg gaaaaacttt gtctattgaa acaaatattt tagagtttga tagagatatt
     2461 tatggggaaa atataattgt tgaatttttg gaaagaataa gagatgaaag aaagtttaat
     2521 tcaatagatg acttaaaaaa tcaattgtat aatgatacaa attatgtgta tgaaaactat
     2581 gtttgcaaaa acaagtaatg tatgataaaa tgattaagga aaataaccat tactcggcat
     2641 ttgaatactc caatcaatgc ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta
     2701 aatgataaac aaatcagaaa taataaaaga atatggaaga gctgaagggg atactggttc
     2761 tccagaagta caaatagctt tattaacagc tagaataaac gagttaaatg gacatttaaa
     2821 aactcataag aaagaccacc attcaagaag aggactatta aaaatggttg gtagaagaag
     2881 aaacttatta gcttacttaa aggaaaaaga tcttgaagga tatagagcat taatagctaa
     2941 attaggatta agaaagtaat ttcattcttt ataaaaaagc aggtatagtg tatacctgct
     3001 tttttatagt taattcatta taaagtaaaa tagttaataa taaaagtagt ttatatgtta
     3061 taaaaaatta taaaatgaat aatattgggt aagaatatta aaatagtata caaatacata
     3121 atcaatttaa aaggaaagac aggataaata tagaaatttt ataacaggat aattagttgg
     3181 aataaggagg atattatttt gaaaataaag ttagtatgcg atagtttgtg cgatattcca
     3241 gatgaaatat cagaaaaaga ttatatagag atagttccac ttacagttat ttttaatgat
     3301 agggaatata tagaaggagt agatataaag aaagaagagt tttataaaaa agtaaaagaa
     3361 atcaagcaaa tacctaaaac ttcacaagct acatatatgg agtttaaaga agtgtttgat
     3421 aagttcataa cagaaggtta tcatataatc tgtatgacag gggctgcaaa cgcatcagga
     3481 acttttcaaa gtgctatgat agctaaaaat gatgtaaatg aaaatgaaaa aatacatata
     3541 tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc atgtgaactt
     3601 cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac tcgcagtagt
     3661 gtaagattgc tttttgcacc atatacactt gattttttaa agcagagtgg aagagttcca
     3721 gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt ctttgataat
     3781 ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag taaattggtc
     3841 gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac aataggttgt
     3901 ggagataatt tacacgattg tgaaatatta aaagatgaag taaataaaag aataaaagca
     3961 agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg gccagatata
     4021 ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat atgtgaatat
     4081 aattttatat tgttaattta tagtattttt gataatatag taaatagagt gatatacgtg
     4141 ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta aaatggattt
     4201 tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcta gtggtagttg
     4261 tatagttcga tacagtgata gtatggtaat ggtaaataca actaaatcag ccaaacctag
     4321 agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat actctgtagg
     4381 taagatacct ggaggttttt taaagagaga aggcaagcct tcagagaaag ctatacttac
     4441 atcaagattg atagatagac caataagacc tttattccca aaaggattta gaaatgatgt
     4501 tcaggtagtt gcaacagttc tatcagtaga ccaagattgt acaccagata tagtagcaat
     4561 gataggttct tcaatagccc tttctatatc agatatacca tttaatggac caacaggttc
     4621 agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga
     4681 aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga tggttgaagc
     4741 cggagctgat gaagttccag acgaggtaat gcttgatgct attttatttg ctcaccaaga
     4801 aataaaaaaa atagttgagt ttatagaagg tatagttgca gaagttggaa aagaaaaaat
     4861 gcctgttgaa ttataccatg caggagaaga aataactcaa ttggttcgcg aatttgcaac
     4921 tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg aaaatatgga
     4981 tagagttaaa gaagaaactt tagctcactt tgaagaaact ttagaagatt ttgaagattt
     5041 tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa gaaaacttat
     5101 agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac ctatatggtg
     5161 tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag gacaaactca
     5221 agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaactag atgggttaga
     5281 tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata gtgttggaga
     5341 agctagacca tcaagaggac caggaagaag agaaatagga catggtgctt tagcagaaag
     5401 agctttatta ccagtaatac catctcaaga agaatttcct tatgctatca gattagtttc
     5461 tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggct caacattatc
     5521 tttacttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa tgggacttat
     5581 aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag accatttagg
     5641 cgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac aaatggatat
     5701 aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag ctaaagaggg
     5761 tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac cagaactttc
     5821 accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa aagatgtaat
     5881 aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta agatagatat
     5941 tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta agaaagctca
     6001 agaacttata aataatatag tagttgaacc agaagtagga aaaacttata aaggtaaggt
     6061 aagtagaata atgaactttg gagcatttgt tgagatactt ccaggaaaag aaggattact
     6121 tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat tgaatatagg
     6181 ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta atttatcaag
     6241 aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat aattaaaagc
     6301 aaagtcttag gattttgctt ttttaaatac aaaaaagtag ataattttaa aatgatagtt
     6361 atggatggct tatagttctt tttctgtcag gctttactct tataaagaag ctatttaaaa
     6421 gctgattaat agagagcttt attgatatat atagaatcac ttaagttata tttagtgcat
     6481 aaacagagct tatttttaat agtattaaat aagaaacaat ttgtacataa ttagggggag
     6541 ttttaatgat aatgatggta cttaataaga gtaagctcaa aaaaatcata atactaattt
     6601 tagtagtgtt attagtatta tcaggagggg taatggtatt aaagttattt aacaacaagc
     6661 cagtatttaa tttatttaat ggtgtagatt taccatatga aagaggtaca aaggataaaa
     6721 gtggatatgt tgcattaacc tgtaatatag accttggatg ggaaacagag tacgtagaat
     6781 ccattttaga aacacttaaa aaagaaaatg taaagattac atttaatgta actggaaaat
     6841 gggcagaaaa gaataaggat gaattattaa agataaaaaa acaaggacat gaaataggta
     6901 atcatggata caaacattta gattattcta cattatcata tgaagacaat tatgagcaaa
     6961 tagagacctc taaaaagata attgaagaaa ttataggtga aaaaactaaa ttttttcaag
     7021 caccagcagg ttcttttggt ccagaaacag taaaggctgc taaagcatta gggtatactt
     7081 ctatcaaatg ggatgccgat actatagatt ggaaatacaa agaccaacca gaggtaatca
     7141 ttgatagaat gaagaaaaaa gacattaaag atagtagtat aattttaatg catccaacca
     7201 acgcaacgac aaaatgtata gatgatatta tagctattgt aagagaaaaa ggactaaaac
     7261 caggaaaact tagtgatgta tttaagtaga caaagtataa atatttggtt aagattgtat
     7321 aagttgtagt tgaattaatt aaaagactga cttataatgt aatacatata aaaaataaat
     7381 ataattaaaa catagtgttt tcaataatcc aatatgaaac aaagaataaa ttgttaggag
     7441 aatgtaatgt ataagacaaa aatactggaa aatgggctta caataattgg tgaagaaata
     7501 ccatatctaa agtcaataac attaggtatt tggataaatg caggctctag gatagaagaa
     7561 gcacaggtga gtggaacttc tcattttata gagcatatga tgtttaaagg aaccaaaaac
     7621 agaacatcaa aagagattgc tagttcaata gataatttgg gtggtcaaat aaatgcattt
     7681 acaagtaaag aatgtacatg ttattatgta aaactaatag atgagcatat agatacagga
     7741 attgatgtat tgagtgatat gatactaaat tctaaatttg ataaaaatga tatagataag
     7801 gaaagactta taatattgga agaacttaag atgtatgaag actctccaga tgacttatct
     7861 tatgatttat tagtggaaaa tatatatgct aatgatggat taggaatgaa tataattggg
     7921 acaaaagaat ctctatacaa tataactaga gaatccatgt tagaatactt aaataagtat
     7981 tatattccaa ataacgctgt tatttctata gctggaaatt ttaactttga tgatatggtg
     8041 gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa atttaagtat agacataagt
     8101 gaggctaagt ttaatccttg ctttatatca aaaaataaag acactgaaca ggtgaactta
     8161 gctatgtgcc taaaaggtat tccttttgaa aatgatgaag aagtctattc aatggcagtg
     8221 gtaaataata ttttcggtgg aagtataagc tcaaggcttt ttcagaaaat aagggaagaa
     8281 aaaggtttag tatattcaat atactcatca cagacattat ataggaaatg tggtgaatta
     8341 ggtatatttg caagcatgag tacagaaaat ttacaagatg tatataattt aataaagaaa
     8401 gaaattgaaa atataagaga aaactatctt acagaaaaag aaatatctga aagcaaggaa
     8461 cagttaaaag gaaattatat attggattta gaaagtacta gcagtagaat gatgtctact
     8521 ggtaaatcta tgttacttag taaaaaagta aaaactacag atgaaatact tgaatgtata
     8581 aataatgtta atattaattc aataaagaag gttgttgata aggtattcaa tatcgaaaat
     8641 attggtacat gtatagttgg aagagatgta gaaaagattc tacacttgga ttaatattga
     8701 aaaaagtaaa tctctggagg tataaaatat gaatttatct gaaatagcag gtaaggaaat
     8761 agttaacttg gtcacaggag aaagattggg ggtcattgga gaatgtgacc ttatactaga
     8821 tgaaactaca ggcggtatat tagcgcttct aattccaaaa gaaaaaggat tttttgccag
     8881 aagaaaagat aagtcatttt tagaagtacc atggagaaat gtaaaaaaaa taggtaatga
     8941 catgataatt atagaatatg agggatatat ttaggaggaa aaaatgagta ttttagtgca
     9001 aaagtttggt gggacatcag tagaatcata tgaaaaaatg aatgaagtat gtaagatagt
     9061 aaaagcatac aaaaaaaatg atgaagaatt acaattagta ttagtagttt cagcaatggg
     9121 aagaaaagga gctccatatg ctacagatac acttataaat ttgtgtagtg cagtaaatga
     9181 tgagccaagt aaaagagaac ttgatttaat aatgtcttgt ggagaaataa tatcaggaac
     9241 aattcttgct aatttattga atgcacaggg gatagattct gttttcttta ctggtcagca
     9301 agcaggtata attacaagtg atgaatattc taatgcaaag ataaagtata taaatcctaa
     9361 aaaggtaaaa agagctttag atgatggtaa ggttgtagtt atagctgggt tccagggtgt
     9421 aacagatgat ggagaaataa ctactcttgg aagaggcgga agcgatacat cagcagttgc
     9481 tattggtaaa gctttagaat gtgaaacagt tgaaatttac actgatgtgg atggtataat
     9541 gactgcagac cctagagtag aaccaaacgc taaagtattg agttttatag actatgagga
     9601 agtattccaa atggctgaca aaggtgcaaa agtaatacat ccaagagcag tacagttggc
     9661 taaaagtgga aatataactt tagccataaa gaatacaatg aatccaactt ttgaaggtac
     9721 aaaaataggt tctttatgta gacacttaga agacaatata gaatatgaac aagaaagaga
     9781 ttttaaggtt gctgttgcaa ataaagatag cgtagcacaa gttaagatta agtcagcaga
     9841 agaagtgttt acagaagtat taaatgagat agaaaagaaa cttataacta tagatatgat
     9901 aaatttcttt atatcagaaa aagcatttgt tgtagaagat gctgatataa aatcattaaa
     9961 ggaaatacta gataaatttg aattagatta tgaagtaaat agagactgtg ctaaggtaac
    10021 attgatatgt tcaagaattg ctgatgaaat gtctggtatc atgtcaaagg tagttagagg
    10081 attatcaaaa gcaggggtat ctcttttaca aacatcagac tcaaatatga ctatatcttg
    10141 tttagttagt gaagaagata tgcatacagc agtacatgct atacatcaac aattttattt
    10201 aaaataaact gtataataca attatttttt aatcaaattt aagaatttca tatatatctt
    10261 aatatatttg atttatttgg taaaactaaa ttaaatataa ttttgatagg agttttttta
    10321 tgaaagatta tagagaagat aatgaaagat ttgataaaga taattgtagt gaagaatcta
    10381 gttgcgaaga gagagaatgt ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc
    10441 ctaaagatat tcaatgtata actataattg gagaaattga gggacatttt ataggaaatc
    10501 ctcaaaaaaa agctacaaag tacgagcata taattccaat gctatactca attgaggaaa
    10561 gcaatgatgt taaaggagtg ttagttgttt taaacactgt tggaggagat attgaagcag
    10621 gtttagctat tgcagaactt ttaaacagta catctaaaaa agtagttact cttgttctag
    10681 gaggaagtca tagtataggt gttcctcttg ctacagcagg agattactca tttatagcac
    10741 caacagcaac catgattata catccagtgc gaactactgg tcttgtgatt ggtataaatg
    10801 agacttttga gtattttaaa aagatgcaag atagaataat tcaatttata ataagaacat
    10861 caaatatcaa aaaagatgta cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg
    10921 atgttggtag tgtattaata ggaaaagaag ctgtggatta tggtcttata gatgaagttg
    10981 gaggactaaa agaagcactt aaaaaattaa gagagcttat aaaagaatct gaggaagaaa
    11041 aaaataatga ttaaaatttg tttaaataat aaaatttatg atataatgat gtaataaagt
    11101 aggagtgtta gtgttaaggc attagcactt tttgctgtga aggggtaaaa cttgctaaaa
    11161 ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag
    11221 ttttaatgca gaatatcata atttaataac aatatttata ggtgtatttt tattatatag
    11281 tttaaattct aattctatgg gatggatacc tgtgttaatg caaaatcttt ttaagggact
    11341 atttggaggg ctttcaatag caataccatt tatagtaatt ataacaggac ttttaggatt
    11401 ttttgatgga aatgaatata tatataggct aagaaagact aaactatact acataattat
    11461 tttatttata tttgtatttt atggattatt aaatgcagga actcttcctg tagatagccc
    11521 actaaaaggt aacatgtttg atgatgttat gaaattgggt gtaagtggac aaggtagtgg
    11581 tttgatagca acaactattg cgtattatat gagtaaaata tttggtatag caggtggatg
    11641 gttaataagt atcttcgcac ttatactttc tgtaatgttt atatttaata tttctataaa
    11701 agatttggtg tcaaatgcaa aatcaaaatc taaagcatct aaagacagca atttaacgtt
    11761 taaagataaa attgcaaata tgaaaaaatc tgcaatagat atgatgactg atgaagtaga
    11821 tgatactact ataaataaaa aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa
    11881 taatgatgat gatgaagatg aatattttga agaagaaaat agtgatggtg tagatgataa
    11941 aactataaaa atagttggat ttaataaggc agaagatgag tatttagaaa tattagaagg
    12001 aactcaaagt atgccagagt tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa
    12061 tccagttgta gatactaaac cagaaaaaaa agttgatata gctaaaccta atttaaatat
    12121 agaaaaaact caacctatga gtattgtagc agagccagtt aatgaggatt attcaaacta
    12181 taaaaagcct agcattgagc ttttaaataa agtaaataag aagtctgatg aaaatggtaa
    12241 aaagaaagtg ttgaaaaatg cttctctttt agaaaagaca ctatcagatt ttggagtgga
    12301 agcaaaaatt aatcaagtta cagtcggacc tactataaca agatatgaaa tacaaccaag
    12361 tccaggagtc aaggtaagta aaattgtaaa tttaactgat gatatagctt taagtttagc
    12421 agctaaaagt ataagaattg aagccccaat tcctggtaaa agtgcaattg gaatagaagt
    12481 tccaaatgaa gaagctcaaa tggttggtgt gagagaagtt ctagaaagtg aagaatttaa
    12541 taattttaac tctcctcttg caatgggact tggaaaagac gtagcaggaa aaattataat
    12601 tggagatata ggtaagatgc cacacttact aatagctggt tctacaggtt caggaaagag
    12661 tgtttgtgta aatacattaa taagtagtat tttatataag gcaaatcctg atgaagtaaa
    12721 attactatta atagacccaa aagtagttga acttgcaaat tataatggaa taccacatct
    12781 attgatacca gtagttactg accctaaaaa agctgcaaat gctttaaatt gggcagttac
    12841 agagatgaat agaaggtaca aattatttgc agatgctcaa gtaaaagatg taacaagtta
    12901 taatgagaaa gcggaagaaa aacttcctaa aatagtaata ataatagatg agttagcaga
    12961 tttaatgatg gcaagtgcaa atgatgtaga agattatatt tgtagattag ctcaaatggc
    13021 aagagctgct ggaatgcact tgattgtagc aactcaaaga ccatctgttg acgttataac
    13081 aggggtaata aaggctaata taccatctag aatagcattt gcagtatcat ctcaaactga
    13141 ttcaagaaca atccttgata tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt
    13201 attctatcca cttggtgcag caaaaccagt tagacttcaa ggggcattca tatcagagag
    13261 tgagtcggaa aaagtaatcg attttgtaaa aagtcaggtt aaagatggaa taaaatatga
    13321 agaagatata attgaaacta tatctaaggt taatacttca aagggtagtg atgaggatga
    13381 gtttttgagt gaagcgatag aatttgtcgt ggaaagtggt caagcatctg cctctatgct
    13441 acagagaaga tttaaaatag gattcaatag agcagctaga cttatagatt ctatggaaga
    13501 aagaggaata attggagcaa gtgagggaag taaaccgaga aaggtattaa taagcaaaca
    13561 ggatttgcag aatttagaag gtgaatagaa ttgtcagtta taaaaataga aaaagcactt
    13621 aagctagata aagtaatatt tgtagatgtg agaactgaag gagaatatga agaagaccat
    13681 atattgaatg caatcaatat gcctcttttt aaaaataatg aacataatga agttggaaca
    13741 atttataaga tgcagggaaa acatgaagca atacaaaaag ggtttgacta tgtatcgtat
    13801 aaattaaagg atatatattt acaagcagca gaacttgcat taaattatga taatattgtc
    13861 atatattgtg caagaggcgg aatgagaagt ggcagtatag taaatttatt atcatcatta
    13921 ggtgtaaatg tatatcaact tgaaggtgga tataaggctt atagaaattt tgttttagag
    13981 tatttaaggc atgtaatgga tacaaaaaac tttgtatctg ttcatggatt gactggtaca
    14041 ggaaagactg atttgttaca tttattagat gaaaaaggaa tagacgtact ggatttagaa
    14101 ggcatagcta aaaacagtgg ttctaccttt ggttttataa cttttgataa aaagccacca
    14161 tctcaaaaga actttgaaac aaaactattt gaaagtatat ttttttcgaa agagaattat
    14221 atatttgtcg agagtgaaag caaacgagtt ggaagtgtta cagttccaaa tgaaattttc
    14281 gaggcaatga cacgagaagg acatcatata ttattagaat gtgatattga aaatagagtt
    14341 gatagacttt gtagagatta tatttatgat aaagatgaag gtaatatatt gattttaaaa
    14401 gaatgtataa acaagtttag aaaaagattg ggtaataaag tagtagatga ttatattgat
    14461 ttattagaat caggtgaata taaagaactt gtaaaaagat atcttttaga atactacgac
    14521 ccactttata tgcattcagt agagaaatat aagtatagta agattataaa ttttaatgat
    14581 acacaaaaag ctttagaaga tgttatagac ttatatgaga gtatattgga aggagaaagt
    14641 gaatgttaaa aatagcattg gaatcattag gatgctcaaa aaatttagta gatgcagaaa
    14701 taatgatggg aattttaaat aacaaaggct ataaattaat tggagatttt gaagaagctg
    14761 atgtaattat agtaaataca tgtgggttta tagagtcagc taaacaagaa tctatagata
    14821 cgataataaa ctttgcagag ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag
    14881 ggtgtctagc ccaaagatat tctgaagaat taaaaactga aattcctgaa atagatgcta
    14941 tagtaggaac aggaagctat caaaatatag ataaaatact taaagaatta agtgaaatgc
    15001 atcaaattgt aagccttaat gatatagagt ttgtatttaa tgaagattta ccaagatata
    15061 tatctacacc tagctatatg gcctatttaa aaattggaga aggatgctcc aataattgta
    15121 cttattgtat aataccaaaa ctaagaggta aatatagaag tagaaaattt gaagatataa
    15181 ttaaagaagc taagaaatta gctgaatctg gagtaaaaga acttgtggtt atagcacagg
    15241 atacgacaaa gtatggtttt gatttatatg gaaaagaaag attatcagaa cttttagaag
    15301 aacttgcaaa aatagatgga tttaagtgga taagggttat gtattcatat ccagaatcta
    15361 taacagaaga attgatacaa gttataaaga aatatgataa tatatgtagt tactttgata
    15421 tgccaataca gcatgcaagt aacaatattt taaaattaat gaatagaaaa actactaaag
    15481 aagatatatt aaataaaatc aatctgataa gaagtaatat accagatgct atacttagaa
    15541 ctacaataat agtagggttt cctggtgaaa cagaagatga cttcaaacaa cttgtggact
    15601 ttgttgaaga ggttaaattt gatagacttg gtgcatttgc atattcaaga gaagaagata
    15661 caccagcaga tagattacca aaccatatag atgaagaagt aaaaatacaa agaagagata
    15721 ctttaatgat gattcaacaa aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa
    15781 cttatgaggt gttaatagaa gaacagatag aagataatgt atatacagga agaacacaag
    15841 gtgatgcaga agaaattgac agtatagtgt atgtaaaaag tgtagacaat cttgaagtag
    15901 gagaatttgt aagtgttcaa ataaatgatg caatggaata cgatttaatg ggagatgttt
    15961 tatatgaact tgcctaacaa gttaactttg tttagaatat ttttaatacc agtatttgtt
    16021 ttaattatgt tgctcaatgt accaaataaa tttttaatag cttgtattat ttttataata
    16081 gcttcaatta ctgatgcttt agatggaaaa atagctagaa aatacaatct agtcactgac
    16141 tttggaaaat ttatggaccc attagcagat aagttattgg ttatttcagc acttacatgt
    16201 atgatagaag accatcttgt aagtagttgg atggtaatta taatagtggc tagagagctt
    16261 acagttagca tattaagagc tatagctgct gctgatggaa aagtaattgc tgctggaaac
    16321 agtggaaaac ttaaaacgat aactcaaatg gtatctatag tgtttttatt gttaggtgct
    16381 cagtttgaaa atgtattgat attaaatatt ggtgaaattc tcattcttat agctacacta
    16441 cttacattat attctggatg ggaatatcta tataaaaata aagagctttt tatgtcttct
    16501 aagtaaaaaa ttatgcttta ataaataaaa actaatgatt aataaataaa aaataacaac
    16561 ttaaaatttt aaaaatgtaa aaaagggtat actattatag tatacctctt ttcattttaa
    16621 gaatattttc ttttgtgtat ataattatta ataatggttt ataaataaaa tgtaatgaat
    16681 aaaaatataa tcattagtag cgtttttaaa ttgactaggt ttgatttatg ttatataatt
    16741 aatatataac aagagaacaa atgtttgtag ataaatgaag ggatggtata gagatgagtg
    16801 tagatcaaga aaaattaaaa gcgttgaatg aagctttagg taaaattgaa aaagattttg
    16861 gtaaaggttc agtaatgaaa ttgggagaag caacatctat gtctatagat gttatatcaa
    16921 caggagcgat tggtttagac atagctattg gtataggagg tctacctaga gggagaatag
    16981 ttgaagtata tggtccagaa tcttctggta agactactgt tgcgcttagt tgtgtagcat
    17041 cagctcaaaa agatggagga atagctgcat ttatagatgc agaacatgca cttgacccag
    17101 tatatgcaaa agctttgggt gtggatgttg ataacctaat aatatctcaa ccagatacag
    17161 gtgaacaggc tttagagata gcagaggcat tgataagaag tggagcgata gatataatag
    17221 taatagactc agtagcagca ttagttccaa aggctgaaat agatggagat atgggtgatt
    17281 ctcatgtagg tttacaagct agacttatgt cacaagcact tagaaagtta actggttcaa
    17341 ttaaaaaatc aaattgtgtt gctatattta taaaccagtt aagagagaaa gtaggaataa
    17401 tgtttggaaa cccagaaact actactggag gacgtgcact aaaattctat tcatcagtta
    17461 gattggatgt tagaaaaata gatacaataa aacaaggtga taaagttata ggtagtagaa
    17521 ctagagttaa agttgttaaa aacaaagtag caccaccatt taagcaagct gaatttgata
    17581 taatgtatgg agaagggatt tcaaaaattg gagacctttt agatatagct gctgatgtag
    17641 atatagttaa aaaatcaggt tcatggtata gttacaatga tactaaactt ggacaaggaa
    17701 gagaaaatgt taaaaaattc ttggaagata atttagattt aactactgaa atagatgaga
    17761 aagttagagc attttacaat ttaaatgaag aacatgaaga atcaggtact tcagtatcaa
    17821 aagaaattgt agaagaataa gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc
    17881 acgctcccta ttagcttgac acaatgttga aaaaattata aaattaagtg aggataaaat
    17941 atctaaattt aattttaatt attaggaata ttttttaatc atagtgatga cattttggga
    18001 ttattaacga aggaggtgga tgtcatagat agtgtagtaa tgattgtaat aggtgcagct
    18061 gttggtatta tagctggata ttttgttaga aaaaatatat ctgaagcaaa aattggacaa
    18121 gctgaaaact tggccaaaga aataatagat aaagcacacc acgactcaga aacagtacaa
    18181 aaagaaaaat tactagaagc taaagaagag atccataagt ggagaacaga agctgaaaga
    18241 gaaaacagag aaagaagaac agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa
    18301 gaagtcttag atagaaaatt acaaaattta gaatctaaag aagtaaacct aagtgaaaaa
    18361 ttaaaggtag tggaaaagaa agaagaagaa gttgaagtaa taaaaactca acagttagaa
    18421 aaattagaaa gtatatcagg aattacttct gataaggcca aagaaatcat cttaaccaat
    18481 gcggagagag atgtaagacg tgaaatgtct ataatgataa aagagataga aagtcaagct
    18541 aaagaagaag ctgataagaa atcaagagaa attataggat atgcaattca aaaatgtgca
    18601 gcagatcacg tagccgaaac tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa
    18661 ggtagaataa taggtaggga aggtagaaat ataaggactt tagaaacctt aacaggtata
    18721 gaccttataa tagatgacac tccagaagcc gtaattttat caggatttga tccaataaga
    18781 agagaaattg caagaattgc tctagaaaaa ttaatagcag atggaagaat acatccagct
    18841 agaattgaag aaatggttga aaaggcgaga aaagaagttg acaatattat aaaagaatat
    18901 ggtgaacaag ctgcatttga aactagtgta catgggattc atccggaatt aataagatta
    18961 ttaggtagac ttaactatag aactagttat ggacaaaatg tacttaaaca ttctatagaa
    19021 gttgctcata tagctggtat aatggctgct gaaataggag cagatattag attggctaag
    19081 agggcaggtt tattacatga tataggtaag gctgtagacc atgaaatgga aggtacacat
    19141 gttgaaatag gtatggattt acttagacgt tataaagagt ctaaagaagt tatacatgcc
    19201 atgagtactc atcatggaga ttacgagcct caaacagtag aggctgtatt agttacagca
    19261 gcagatgcaa tatcagctgc tagacctggt gcgagaagag aaactttaga agcatatata
    19321 aagagacttg aaaaattaga agaaatagct aattcatatg aaggtgtaga aaaatcattt
    19381 gctatacaag ctggtagaga aataagaata atggtaaaac cagaagctat aaatgatgaa
    19441 gaaatacatc tattggctag agatatgact aagaaaatag aggatgaact tgaatatcct
    19501 ggacaaataa aagtgagcat aataagagaa acaagagcta tagaatatgc aaagtaaaag
    19561 aaataatatc taaataataa gaaaatattt ttagaaattt aaatttgcca caagatatga
    19621 taatatatct tgtggctttt aaattgttta agtttaattt tatataatgc aaaaataatc
    19681 taagtgctaa aagtgctact tacaaaagat taataaaata aagatttata tatggattat
    19741 ttatataatt atccaagttt gtttgatata atgaagttgt aaaatcaata ctttactaca
    19801 aaataaatag taaatactac agtaaaatct aaaaatagga tataatagta ttaatataag
    19861 aagattataa ataaaacctg ctaatgaaaa tagattattt ttagcaggtt tttaaatttt
    19921 ttataataaa tcttattttt agtcaacaag atttattttt tttaaaactt tacttattat
    19981 tttaaaaatt aattatgaaa tttatcgttt tattatttac agaaagtatt ctataacaat
    20041 aaatatctat atttattgca aaattatttt agcagttttg tatttatttg tatttatttt
    20101 gaatttattt actaaatttg cctataaaaa gtatatataa aaatatagtt caaaatggta
    20161 taattaagta taacagaagg aggtaatgta agtgagccaa tacataaata taaattctca
    20221 gttagacgcc ctaaaattga aggagttcga gaaaatattt tacgaaaata tcaatgagga
    20281 cgttaaagta gtgccaaaag ttactccttt taaaggaata aataccgatt tgttgtatgt
    20341 taaagatgga agaattttat ttataaaatt tatggataca acagaagata tattttttat
    20401 tttagaagaa gagttacttg aagttatgaa tgaggaatat gaacttttga aactaaaaat
    20461 gggacaaaag aatagaaata taagttataa ctacgtttac attatgccat acgttgaagt
    20521 tgaggaaaca tatgaatttg aagaatttgt taataataat ataatagata aaaataagct
    20581 tcaagatata atgaataagg gttcgctgga ggaatatctt aatgatgaga atgatgaaat
    20641 aaacttaaat ttatttttac tagatatatg ttcagaatac tatataataa atgataaact
    20701 tcatctaaat gaaaaattta aaaaaatttc attctacaat gatgactata agtatacagc
    20761 aactatgatg gaagacgttc aaattaaaga tgttgtttct attaaatatg ggaatacact
    20821 tattgaaggt ggttctggta taggcaaaac agcaattatg ctttctaggg ctataaaatt
    20881 agcaaaagta tatccacatc ataaattagt catatttaca catacaaaac aattgtgcaa
    20941 tgagcttcga gaaaggatag agttattata caaagataat aacaatttag aagttcacac
    21001 atttagttct tttatattta aactagctaa aaaattcaat ttaattgtag attataatat
    21061 gttaaaaaat gattacgaaa agacctttaa taatcttgta aaacaagcac agaacattat
    21121 aaaaaataaa aatatgttca aggctatttt tattgatgaa gcagaaagct ttttagagta
    21181 tgaaatagat tttataaggg agtttttata taaaacaaaa ttcatattta atgtatgttc
    21241 ttgtaactcc ttgaatatct ctaatagatt gaatatattt aaaaaattat ataatggtat
    21301 agaatttgat gataagatta tcttaagtaa aaattataga caagctaaag agattgtaga
    21361 ttttacaaat aaattttcaa ataattctaa ctcatatata aacgaattaa gaccaaatac
    21421 agaatttagt actttctttt atactaaagc acttagaggt ggaaataagt ctgttgatat
    21481 aataaaagta agtgaccttg atgaccaaat aagttctgta atctgggaaa ttgagtattt
    21541 aataagtaaa aaaggtcttg attattctga agttgctatc gtttaccctt acaataagaa
    21601 gaaattaaaa agtggaaaaa ccatttactt tcaatatatg ttaaaaaaag cattggaaga
    21661 ggcaaaaatt ccttatatat gtgcagaaga taatttgaca aatataagta agaaggtagg
    21721 aactacaata gcaaatatat atgcaataaa gaatttagag tataaagcgg taatagtatg
    21781 tgaattagag atgttatata atcaaactat aaatgatatt gaacaagatt atcaagtaaa
    21841 tgactttgta ggagatttaa ataaggttta tttagctatg agtagagcaa cagactacct
    21901 aagtatagta acaactttta atgaagaagc aagtgatata ataagactta ttacagaatc
    21961 aaaagatatt tagtaaaaaa atatttgtaa tttaagaaaa taaaaagaga ctgagatttg
    22021 taaaataaac tttaccaatt cagtctctta ttttttagtc tactttccca atattatcaa
    22081 gaggtaatag tctcaccata accttaccaa gaacttcatt ttcatcaacc atacctacat
    22141 cagggaatct actatcatta ctattttctc tattatctcc cattgcaaat agtttacctt
    22201 ttggaacaac agtatctata tctccagaag tatagttatt gtgtatatat ggttcattta
    22261 ataattttcc atttacatac actttagaat ttgatatttt tattctgtca ccttcagtag
    22321 ctataactct ttttattaag tcttttttct ttcctgtttc tccatcaact aaattggttt
    22381 taaaaactac aatatcgcct ttttcaggtt taccaacctt gtatgatatc ctatttaaaa
    22441 tcagataatc tttatcatct aaagtaggat acattgattc tccacttaca atagaaggtc
    22501 ttataaattg tagaattaca aatgcaagta caatagccat agctattgac ttaatccaat
    22561 caaatatttc ttttttaaca ctcataactt ttctccttat catttgttat taaattagaa
    22621 tacattataa tagaaagaaa taagcaagaa ccactatacc caagactatt ctataataac
    22681 caaatacttt gaagtcatgt tttttgatat agtccataaa aaatttaata actacaattg
    22741 aaacaacaaa tgctactaca gaaccaacac ctaaaataag ccattcaaat ccagtaaatc
    22801 caaatccagc ttttagaagt tttaatgcac tagctccaag cattgtaggg atggctaaga
    22861 agaatgaaaa ctctgctgct acatatctag aagtaccaag taatactgca cctattatag
    22921 tagaaccaga cctagatgta cctggtataa gagctagaca ctggaataat cctatacaaa
    22981 gagctaattt atatgtaact tgagaaaaat ctttatactt aggtttttta tttctctttt
    23041 caagccctat cattattata ccatatacaa taagagcaat agcaactact gttgaattaa
    23101 agaataatct atctatatca tcttcaaaca gtattccaat aactccagat ggtataactg
    23161 caattataac cttaagccat aaacggactg tttctttttt ctgcttaata ttctttgcgc
    23221 tatcaaaagg atttaatttt ctaaaaaata taacaagtac agctaatatt gaaccaaatt
    23281 gaattacaac taaaaatgta cttataaatg tatttgaaaa attcaatttt ataaactcat
    23341 caactaatat catatgccct gtactactta ctggtagcca ttctgttatt ccctgaacta
    23401 tgcccaaaat gactgcctta aaaatttcaa gcaaactaaa tgtttccaaa tcaaaatacc
    23461 tccaatacaa atttatagta gcatatttta ttcatagatt aataaaaaaa tgccaccttt
    23521 tactaactta taataccata gtactgtgta gtgcacaatg gaagattgat attttcataa
    23581 aaccttcata atataacaaa ataaacttaa aagttatcta ttgaataagg ggtatatttg
    23641 agggggaaat atatggtata gtaaaagtat agctatttta ttttatgaaa aggaaaggag
    23701 ttattatgta tagtacaaaa atactagatg attatgagta tgaaactttt aatgtttttt
    23761 ctacaaaatt aaatccaaac acgaaacacg attatttatc aaaggttata ctgtttaaag
    23821 aatttttaaa agggaaagaa ttgatttatg ctacgaaaga agattgtaaa aattttgtag
    23881 actatataca gactaaatat gcgaaatcaa cttgtgaaaa gatatatagt tatttacata
    23941 gtttttataa cttcttaaaa aaagaaggat acatagatat aaatccattt agatatgtag
    24001 aaaaacccac agtaacaaga ataaaaacaa aagatgatgt tctaagtata caagaaataa
    24061 ataaacttat aggtatccta ccaaaattaa atataagaga tagagtaata atagtctgtc
    24121 tagttactac tgggtgttta ttaaatgaat tagttagctt aaagtggaaa gaccttatgg
    24181 tagatgaaaa cgataactca tatgtaaggt taggtaaggg aagaaaagaa agagtagtta
    24241 aattacatcc atactttttt aaacttttag aagattacag gaattattct gggcttccag
    24301 aggtaataat acctagtgat gattttattt ttacaactca aaagagtaat tctataacag
    24361 atagaaatgt cagacttata gtcaaaaaag cattagattt agctggactt tctcaatatt
    24421 cagcaagaga ttttagacat tcatttgcag caataagttt aaggcttggg gcagatgaaa
    24481 gtgatgtaaa aaatcagctt ggatggagtg ataaatacta tgcaattaga tataagtatg
    24541 tattaaattt tgttgatagt gaaatagtgg attacttaat tgaaaaagat aatttggata
    24601 taaataaaaa atagtaataa ataaaaaata ctatatataa atgtgaacaa aaaataaaaa
    24661 agtcttgtta tagttcgtaa aattatatat aattatttat gtactaatgt gtacacagta
    24721 atcacacgac aaaagattta aggaaggtgc taatataatg atagataata aatatactac
    24781 ttactcaaat ccacttacag acagatattg tagcaaggac atgagttata tattttctcc
    24841 acaatttaaa ttctctacat ggagaaggtt atgggttgca cttgcagaag gtgaacatga
    24901 gttaggaatc aatataactg aagaacaatt agatgaacta agagctaata tagataatat
    24961 aaattttgat gaagctagaa aaatagaaaa agaagtaaga catgatgtaa tgtctcacgt
    25021 aaaagcttat ggtttacaat gtgaaaaagc caaaggaata atacatttag gagcaacatc
    25081 tgcatatgtt ggagataata cagatgttat acaaatgaat gaagctttaa aacttctaag
    25141 agtaaagctt gtaaatttaa taaacaattt aaaagaattt gcacttaagt acaaagatat
    25201 gccagctctt ggatttacac acttccaagc tgctcaactt acaacagtag gaaaaagagc
    25261 ttgtttatgg actcaagatt taattcttga tttggaagat ttaaactata gaatagacaa
    25321 catgaagtta agaggagtta aaggtactac tggtactcaa gcaagttttt taagcttatt
    25381 tgaaggagac cacgaaaaag taaaagagtt agataagaaa atttgtaaaa agatgggata
    25441 ccattcttcc tatgcagtaa gtggacagac atatactaga aagttagatt atcaagtaat
    25501 aagcatacta tcagggatag ctcaaagtat gcataagatg actaatgata taagactttt
    25561 acaaagttta aaagaattag aagagccatt tgaaaaaaat caaataggtt catcagcaat
    25621 ggcttataag aggaatccaa tgagaagtga gagaatagca tctttatcta agtatataat
    25681 ctctgaatct ataagccctg caatggtaca agccactcaa tggttagaga gaacattaga
    25741 tgactcagcc aataagagat tggcaatacc tcaagcattt atggcagcag atgctatact
    25801 tgaaataggg ataaatgtaa ctgatggttt agtagtatat gaaaatatga taaatagacg
    25861 tgtaaatgaa gaacttcctt ttatggctac agaaacaata cttatggaag ctgtaaaaag
    25921 aggtggagat agacaggaat tacatgaaat aataagagaa tattctatga aagctgccta
    25981 tagagtaaag catgaaggca aggataataa cttaattgaa cttataataa atgatgattc
    26041 atttaaaatg agcaaggaag aaatactatc tataatggac ccaaaaaact ttataggtag
    26101 agctccagaa caagttgtag aatttgtaaa tgaagttgta gaacctgcta taaaagatta
    26161 taaggaagac ttaggaaaag tagatgtaga cctaagagta tagaattgta cagataaaat
    26221 ataattaaaa acggctatct tatttataaa taagatagcc attttaatgg gctagcgtct
    26281 tctctccacc gctgtgaaga gttcttcttt acttactata ccatgaattt aataaaatag
    26341 ctatataaat aatatttaat taggaataat ataaataaaa ataaaaaaat gataagatta
    26401 ctaattaaat ttaaacattc agcaaaaact attgtacttg caatattttt aatgtaaact
    26461 gaaataaagt tatttagata atttaataaa ttttaaaaca accgaggtga ttatatgaac
    26521 tatatagtaa aagataccat aagtttttta gtaaaaacat ttcctaagat ttattctagc
    26581 ctgtacttag aagacctaaa aaaatttgca ccagattata atgtaaataa aacacaatta
    26641 agagcattag ttttcataaa aaattatgga gtaataagta tgactgattt atgctctaag
    26701 ttaaatatag aaaaaggcag tttaactagt atggttgatg atttaactga taagaagtat
    26761 gtaacaagga aaagagattt agttgataga agaaaatatc taattgacat tactgaagag
    26821 ggagataaga tagcaactga ttttatggat aaattaagtg atggtttagg agaaaaactt
    26881 tctaaattaa ctgaagaaga cagaaaaaaa tatttagaag caataaatac tcttcaatat
    26941 atattaaaca aagaagaatt tagatagaaa aaatataaaa atttttgtat ttaatggtac
    27001 taagattaat tctttgttat tcatttaact atataattat aagtctaaat acaatctaaa
    27061 aagtacatct taaattttct tgatgtactt ttttataatt tctctaacta aggcaagaca
    27121 agttttagat gctttaatta aagtgaataa agattactgg ccaacactat ataaagaaaa
    27181 tgaaaaatag tcaggtctaa gtcttaatta atatattaat taagaaatgt ccccttttca
    27241 aataatatct gaaaagggga cattttatat cattaaattt atataaatta agtttaaaaa
    27301 attatttttg taaagctaca tattgctcta aaccttcttt taataatgta gcagctctat
    27361 gaaggtcttt ttcatttaag atgtaagcta atcttatttc atcacgtcct aaacctggag
    27421 tagcatagaa tccttcagca gggcaaacca taactgtttc attatctttg ttaaattctt
    27481 ttaacatcca aattgtgaaa tcttctgcat tttcaactgg taatttagca actatataga
    27541 aagcaccagt tggtttttta catataacac cttcaacctt cattaattca ttgtataata
    27601 catctcttct ttttttgtac tcttcattaa cttctttgaa gtaagaaaca ggagttttgt
    27661 ataactcaac agctcctatt tgctctaatg taggaacaca aagtcttcct tgacataatt
    27721 taaggatttg agcgataaga cctttatttt tgcaagcgat agaaccaatt ctagcaccac
    27781 aagcactgta tctcttagaa acactgtcta ttattataac tctatcctgt acaccctcta
    27841 agttaccaca gcttgtatac tcaagcccat cataaacaaa ttctctataa acttcatctg
    27901 ctattatcca taaatctttt tctttagcta tttcagctag gatattaagt tcttcttttg
    27961 tataaattgc accagttgga tttcctgggt tagaaagaat tatagcctta gttttatcat
    28021 ctactttaga caatatttct tctttagatg gaaggtggaa accgttttct gcctttgttg
    28081 taacagcatt aacttctacg tttacagatt gaccaaatcc attgtagttt gtatagaatg
    28141 gttctggaac taatagatta tctcctgggt cacaaactgc catcatagtg aaaagtaaag
    28201 cttcactacc accattagtt actaataatt catctttttc aaaattcata tcgtaagttt
    28261 tatagtaatt ttgaagtgct tctaataatt ctggaatacc ttctgatgta gcatattcta
    28321 agacttcgct atcaaagttt ttaacagcat caaaaaatcc tttaggagtt tttatgtctg
    28381 gttgtcctat gtttaagtga tatactttga ttcctttatc ttttgctgct tgtgcaaatg
    28441 gaacaagttt tctgattgga gaggcttgca ttgcactaac tctgtttgaa taattcattt
    28501 taataccccc tatttaattt aaatacgtat attgtattat gtaaattgta tgtttgaata
    28561 caagacatct ttacaagata ataataacat acttatgggt aaaaatcatc aaaaaaaatt
    28621 aatatagctt taaaataaac ttttaacaaa ttatagtaaa atacccatgt ttatgccact
    28681 aaattatgta ttcataaatt aatttatagt aagataatat tctgatatac tgatagtgat
    28741 tttttagtta ta
//
LOCUS       DAENCV010000045        28626 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAENCV010000045 DAENCV010000000
VERSION     DAENCV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.045"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            76..522
                     /locus_tag="KQD82_001938"
     CDS             76..522
                     /locus_tag="KQD82_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG4380408.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            524..1618
                     /locus_tag="KQD82_001939"
     CDS             524..1618
                     /locus_tag="KQD82_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG4380409.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMTKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1706..2236
                     /locus_tag="KQD82_001940"
     CDS             1706..2236
                     /locus_tag="KQD82_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4380410.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3173..4549)
                     /locus_tag="KQD82_001941"
     CDS             complement(3173..4549)
                     /locus_tag="KQD82_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380411.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4817..5989
                     /locus_tag="KQD82_001942"
     CDS             4817..5989
                     /locus_tag="KQD82_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380412.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSAGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6003..6650
                     /locus_tag="KQD82_001943"
     CDS             6003..6650
                     /locus_tag="KQD82_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380413.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6652..7161
                     /locus_tag="KQD82_001944"
     CDS             6652..7161
                     /locus_tag="KQD82_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG4380414.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7534..8646)
                     /locus_tag="KQD82_001945"
     CDS             complement(7534..8646)
                     /locus_tag="KQD82_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG4380415.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLAGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8944..9210
                     /locus_tag="KQD82_001946"
     CDS             8944..9210
                     /locus_tag="KQD82_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG4380416.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9455..11182
                     /locus_tag="KQD82_001947"
     CDS             9455..11182
                     /locus_tag="KQD82_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380417.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11182..12972
                     /locus_tag="KQD82_001948"
     CDS             11182..12972
                     /locus_tag="KQD82_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380418.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13134..13442)
                     /locus_tag="KQD82_001949"
     CDS             complement(13134..13442)
                     /locus_tag="KQD82_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380419.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13704..14252)
                     /locus_tag="KQD82_001950"
     CDS             complement(13704..14252)
                     /locus_tag="KQD82_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380420.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14355..15026)
                     /locus_tag="KQD82_001951"
     CDS             complement(14355..15026)
                     /locus_tag="KQD82_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4380421.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15422..16327
                     /gene="garR"
                     /locus_tag="KQD82_001952"
     CDS             15422..16327
                     /gene="garR"
                     /locus_tag="KQD82_001952"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG4380422.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGFNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16463..17845
                     /locus_tag="KQD82_001953"
     CDS             16463..17845
                     /locus_tag="KQD82_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG4380423.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKAPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMILLLSV
                     INGIIPLPGL"
     gene            17916..19352
                     /locus_tag="KQD82_001954"
     CDS             17916..19352
                     /locus_tag="KQD82_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG4380424.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19538..20392)
                     /locus_tag="KQD82_001955"
     CDS             complement(19538..20392)
                     /locus_tag="KQD82_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380425.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20660..20983)
                     /locus_tag="KQD82_001956"
     CDS             complement(20660..20983)
                     /locus_tag="KQD82_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG4380426.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21186..21815
                     /locus_tag="KQD82_001957"
     CDS             21186..21815
                     /locus_tag="KQD82_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380427.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGTKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22143..22652
                     /gene="ruvC"
                     /locus_tag="KQD82_001958"
     CDS             22143..22652
                     /gene="ruvC"
                     /locus_tag="KQD82_001958"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG4380428.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22645..23247
                     /gene="ruvA"
                     /locus_tag="KQD82_001959"
     CDS             22645..23247
                     /gene="ruvA"
                     /locus_tag="KQD82_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG4380429.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23354..24373
                     /gene="ruvB"
                     /locus_tag="KQD82_001960"
     CDS             23354..24373
                     /gene="ruvB"
                     /locus_tag="KQD82_001960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG4380430.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24691..25716
                     /gene="queA"
                     /locus_tag="KQD82_001961"
     CDS             24691..25716
                     /gene="queA"
                     /locus_tag="KQD82_001961"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG4380431.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25728..26213
                     /locus_tag="KQD82_001962"
     CDS             25728..26213
                     /locus_tag="KQD82_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380432.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26292..27413
                     /gene="tgt"
                     /locus_tag="KQD82_001963"
     CDS             26292..27413
                     /gene="tgt"
                     /locus_tag="KQD82_001963"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG4380433.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27547..27840
                     /gene="yajC"
                     /locus_tag="KQD82_001964"
     CDS             27547..27840
                     /gene="yajC"
                     /locus_tag="KQD82_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG4380434.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27898..28260
                     /locus_tag="KQD82_001965"
     CDS             27898..28260
                     /locus_tag="KQD82_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG4380435.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28326..28469
                     /gene="scfA"
                     /locus_tag="KQD82_001966"
     CDS             28326..28469
                     /gene="scfA"
                     /locus_tag="KQD82_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG4380436.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
BASE COUNT        10839 a         3301 c         4867 g         9619 t
ORIGIN      
        1 ttgactatat taattgttta atataatata gttgatatag tcaaacatat tataattata
       61 gaagggaaat ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc atatattatt
      121 tgtaacttgc aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc atataaactt
      181 acgaatggtc tatatttcta tttaatttat attaataaaa ataggaattg ttctttaaat
      241 gatgtttcta cagaatttga agttgataaa gctcatacaa caagaactat atctaggtta
      301 gaacaagatg gatacataga aaaaatccaa aaccctaatg atagtagagc ttttcagttg
      361 agagtaacag ataagggtga ggaagtatta ggggatataa aaaacatatt ttcaaagtgg
      421 gataatcaca taaaaaaaga gttctcggat acagaatata aagaactggt aaagaattta
      481 catgtggtaa aggatataaa aacagctgtg gaggaagaat aggatgtatt gtaatttatt
      541 aaaaccaatt aaaattggaa atttggaatt aaaaaataga gtatcttttg cacctacaag
      601 catgggtctt aaattggaag aaaaaattaa aaagttttct gatattgcta agtcaggagt
      661 agctctgatt actctaggag atgtatctat tagacctagt tttcataagg tagctataag
      721 cctatcggat gaagatggag tcatgaaata caagaaaatt gtagatgaaa tacacaattc
      781 tggtgcaaag gtatcagcac aactattttg ttcagattat gatgttaatt taattaaaga
      841 tactatgaag atgggaatta cttcacatga tgagataaaa aaaataatga atgacggagt
      901 aaaagattac ataacaaata tgaccaaaga agaaattaaa aatattataa atttatttaa
      961 agtaacagca ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc atggtgatag
     1021 gcttgtagga agtttttcat caagtatatt taataataga aatgatgaat atggtggaac
     1081 gtgtgataat agaagtagat ttgcaagcga aataattagt agtatccgag atgaagttaa
     1141 ggacattcca atagactata aatttgcaat aagacaagaa aatcctcatt atggaaatgc
     1201 aggagtttta ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc taggtgtaaa
     1261 cagttttcat gtaacacttg caaatcattc aaaattagaa gatactatac caaccaataa
     1321 tcatccttac tttaaagatg aaggctgttt tttatattta gctgatgagg taaagaaaca
     1381 cacaaatctt ccagtatgtg gggttggaaa attgtctagc ccagatttta tagaaagtat
     1441 tatttcaaat aatagagtag atatggtttc tatgtcaaga caattactag cagatagtaa
     1501 ttggttacag aaagtaaaag atggaagagt agatgagatt aaaaagtgct gttattgtaa
     1561 taagaagtgt gcagatgctt tacaaacacg ctctcagttt ggatgtatac tagattagaa
     1621 tatccttata aaaataaata atttgttaag gttaatatat ttttggtgat ttagataagt
     1681 atttagataa agaggaggag acatcatgaa aagtttagtt ttatattcaa gtttaactgg
     1741 aaatactaaa aagattgctt atactatata tgatgaaatt caagaagaaa aagacattaa
     1801 agatgtaaat gaattagttg attatagtat tgactatgaa aattatgata tagtattctt
     1861 aggctattgg gtggacaagg gaatttgtga taaaaattca aaacaagtgc ttgaaaatat
     1921 tcacaataaa aagatagcac tatttggaac aatgggagca agtaaaaaag gaagttatgg
     1981 agctagtata atagaaaaga tagaatcaat tatcccaaaa gataatgaaa tattaggttc
     2041 attcatatgt caaggtaaga tagcagaagg attgaaagca aaatataaag agatgctaaa
     2101 actttctcct gacaacgaac atattcgcca acaattaaat aaccatgagg aatctcaaag
     2161 tcatccagat gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata tgatgattaa
     2221 agctagtatt gtgtagtagt taaacaataa gttcatataa gatatagaat aaataattta
     2281 gtaaaaaagt atagcaaata atttgctatg ctttttttat gaaatcaaaa aaataataaa
     2341 aatacttgac tgtaaacctt gtttatacat tattttatag aaaataagat agttttaaat
     2401 ataaaaaaag atatattatt gaaaggagat attattgtat gaaaacttat aaaagataat
     2461 tagtttgatt ttaagatata ctaaatatac aattttatag agtgaaaata tttaagacta
     2521 tattatagtt ataaattaat agaatagaga ggtagtgttt aaattgcaat aacagtattt
     2581 ccaaagttgc cacctcaaat tcctatccaa tggaataatg gtgtagctaa ttatgtagat
     2641 aaaaaattta tttatgccta ccctgtcatt tgtgttgtat tcagaatact tttacgtcca
     2701 tttatatgga gatggttaca aatacatgct ttttatagtg atactattgc agattattta
     2761 acgaatttta tgtgttttgt agcgttgtct atagaaatct tctctatact ttttatttac
     2821 aatattgcca gacatgttac aataattatt tttatagata tacttgtatt tattgcttta
     2881 ctgataatag gtatgtataa attatttaaa aaatacaatt aatttttaaa aattaagtaa
     2941 taagtgatag tgtaaaaagt agccactgca aattatggat tgtagtggct attttattat
     3001 ttgtatatga aattattttg tttttctaat aagttacgat atattatggt ttatttcact
     3061 gataaaaggt ttaggtattt taaagtaaaa agttgtatat tctaataaaa tttaaacatt
     3121 ttgattcttt aaaattagag aaaatacatt gcttttatta aaaaattaaa tattaagaat
     3181 ttaatttctg catcttattt agaaaagata agaaatcaac tttatatatt ttttttggcc
     3241 ttcctctttg attatcgagt tttgtattta caatagttgc aattcctgct ttatataaaa
     3301 tactcaatat tctattagca cttctaagag ttatgttcat atagtctgct aatatttctg
     3361 ctgacacatt atttgaattt ctatcattaa tcatacacat gattttagat acatttgtat
     3421 tagatagagg tataaaagaa gcaactttat tggctattga gctatcttca atattattag
     3481 aatttttatt tgaatataaa ggacctattg tatcattagt atcatttact ataaaagtac
     3541 agtttccatt caaagaagca gatttaccat tagcttgacg agcattttgc tctgcatgaa
     3601 tatttgtatt tccaatgccc caacctatat ttaagctata tttaatttct tttaaatctt
     3661 ctgaaataaa aaaatctgta tagttattgg tcatattgtt taacatttct ttagtagtgt
     3721 gtatctctat tttcatatca ttttttttga cctgaactcc atagaatcca tgtcttgata
     3781 gagaatttaa tatagtgtta tatatttgat tttgtatctc atcattttta aatatcaaat
     3841 cttcatttat gtactctgaa ctatttactg taactataca agttgcagat ttatttttgt
     3901 ctaagttttg taatgttatt tcattcacca gagatttaaa tgtattcagg attgattctg
     3961 tagatggaac catgtaaata caatcatatc caagcttatt aagttcacaa ctaatgatgt
     4021 aatttctagt aaaagaaagg ttaatcttat tatctcgcaa taagtctaaa tgtatttcca
     4081 ataatttatc atttaatttt ttaaagtcat atacaggttg agggatatta aaatctttaa
     4141 ttgtatatgg acatttatta gagtctatta atgaatatat atcataataa ttgttgtcct
     4201 ctcttaaaaa atccataaat gattttgtaa agtcaatatc tttttggcta tttaataatt
     4261 tgaataagta tttataaaaa tctctttcat caagctttaa aaaatttaat ggaactttta
     4321 tgtcttttat atttattata agttcactat atggaactat accactgcat acaaatccat
     4381 caaaattatc tacttgctca tagtatatat taggtaaatt attaaaagga ttctcccctt
     4441 tgagctcaat aaatgtaatt tcacaagtat catttaattc tttaagagct tgtttaaact
     4501 tatctgacat aaaataagat gttgtcaaaa aaccaatttt atatttcata aaacctcctt
     4561 taaaatttcg agagtattgt aactaatcaa tcaatttata acctcatttt ataatagtag
     4621 ataaaacgcg tcaatttatt ttacaaaagg ataattgaaa gaaaaatcag aaaatataaa
     4681 aaagactgaa aaatatgttg actgtcaaga ataattgtgc tattttgtaa ttaaggacta
     4741 gtcttttaag atttcctaaa ataaacttag gttttaggaa agaaatacat tttatttgaa
     4801 aataaaggag aagattatga atacatattt aaatgaaatt tctaagtttg ttgatgagaa
     4861 tagagaagaa atagtttcat tatggaaaga gattgttaat atggaaagct atacacattg
     4921 taaagaaagc gtaaataaat tggctaagag attaaagttt gagtttgaaa aagaaggttt
     4981 agattgcgaa ttagtagatg ttggagataa tggtagtaca ttgataggta cacttggttc
     5041 taatataaat aaaaagccta taattttttc aggccacatg gatactgtct ttgaaacagg
     5101 tacatttgga gagaatccat ttaaaataat agaaggaaaa gcatatggcc caggtgtttt
     5161 agatatgaag ggtggtataa taatttcctt atatgttatt aaagctttaa ataaaatagg
     5221 atacaaagaa agaccaataa aaatagtatt ttctggagat gaagagatag gacataagga
     5281 ttcagcaggt gcagatgtta tattgaggga agcaaaaggt ggtctttgtg cttttaatat
     5341 ggaaactgga ttagtagata atagtttgtg tgtaggtaga aaaggaagaa tcggatgtaa
     5401 tatacatgta aaaggtgtag aaacacacgc aggtaatgat tttgaaggtg gaagaaatgc
     5461 tatagaagaa atggctaata agatactacg catacaaaaa ttgactaatt tagatgttgg
     5521 aactacagta agtgtaagtg tgattaaggg tggaagagtg gaaaattcta ttccagaaga
     5581 ttgtagtata aaagtagacc ttagatttga aaaagtagaa gaaatggaga atgttaaaga
     5641 acaagttaga gagatatgta aagaaactta catagaagga acttctacag aagttaattt
     5701 tataagtgaa atgatgccat ttgaaactac agaagatgtt atgagatttc atacatttgt
     5761 aaatgaagtg tcaaaagaaa atggatttgg agaattaaat gctaagagac ttggaggtag
     5821 ttcagatgca agttatttaa ctattgcaaa cgttcctaca atctgttcat ttggtgtaag
     5881 aggggaatgg aaccacacat caagagaata tgcagtagta gattctatgt ttgagagggt
     5941 gaaattaatt tctacagtta ttttaaactt agataaattt gaggtataaa gggggatttt
     6001 atatggatgt aacagctgga gataataagg taacatggaa agggtattta tctttatttg
     6061 tattgatagt attattttca ggagtgttta aagatagtca aggcttcctt aaagcattag
     6121 attttgctaa tctgacaggt tcatttggta ctattgcaga tggtgtagac tttatgggta
     6181 aaggaggaac gggagcaaaa gatggtttct tatttgctct gactttgatt ccaactgtct
     6241 gttttgctgt aggtcttata gatgtagttg aatctatggg aggaatgaaa gcggcatcaa
     6301 agatatttaa tccattgctt agacctctgc ttggtatacc tggaatagca ggaatagcat
     6361 ttgtatcttc atttactagt tcagatgtag cctcaataat gacgaaagaa ttatttgaaa
     6421 gtggggaaat aacagatgat gaaagaacta tatttgtagc atatcaatat gctggttcag
     6481 ctgttatact gaacacaata aatactcaag ctccactttt accaatagca ttattggcat
     6541 taggtccaat aatccttata gagatattct gtaaaatact tggtgctaat ctagttagat
     6601 taatcatagg tgtaagaaat aaaaagaata aggctaagga ggctgtgtag tatgagtcaa
     6661 caaaatactg gaaatgcaat tgtaaatagt aaacctaaga aaactgctgt tgagatattt
     6721 atggggggag caaaaaaagg tttttacata ggtgttgaac agatattacc agccatgata
     6781 atgggttatg taatagtgca gtttttacaa ttaactggtt tagttgatat tttaggaaat
     6841 atttttggac cagtaatggg tatttttgga ctacctgggg aatctgttgt agttttgata
     6901 gcagcattct ttgctaaggc agcaggagca gctacagctg caaacttatt tgcacaaggt
     6961 ctaatcactg caccacaagc aaccatatta attatgccat gtatgttaat gggtactcta
     7021 gttggtcatt atgccagaat agtgcttgta gcaaatgcca gtactaagta tagaatgtta
     7081 ctgttggcag ttcctatatt tgatgcaata gtggggatgt taatagtaag agttttactc
     7141 actgttatgg gattaatgta aataacataa aattatagcc taattataaa tgatatataa
     7201 gaattaatat atgcaagaat tgatgaatag taattgatat atagtaataa aataatatag
     7261 taattaatat gtatattgta tatcaattaa aaaagaagag agtatctcaa agtgaacttt
     7321 ttagttcatg agatactctc tttgtatgaa atcaagtata ttttcataat ttcaacaatg
     7381 aaagagaggc ttatctctat tttgagacag cctcttcttt taaatttata tttctagaat
     7441 ttatgttttt tatttatcca acattaacat tagttattag aaataatttt aagaaaaata
     7501 tactttcatg ttaatatcag aataaatgtg tttttaaagg atgatttgtt ttgaattttc
     7561 acgaatatct acttctatga ctttttctat tgtatctcct ctaaaaccta ataaattact
     7621 tgtcaagttt gcagtggaac aagaatgatt aggtataatt cttattctat caccaatttt
     7681 tatatcagtt tcaccattta ctttaatttt tccaacttct tctgaaagtg aatcaatagt
     7741 aagttctgga tgtccacata ccctaccata accaacaata ctactatttc catgtgcacc
     7801 tttatcaagt ccaaggcact tacttccaca atcaattaaa tagtatccac tgctattttt
     7861 tgagattaca gtgcacacaa cagtaagagc acacatatct tcagtagcac aacctaaacc
     7921 tatctgtata gcatcatagt atacatagtt tccaggtctt acagcagtta tagttttaga
     7981 agcagcttca gattcaactg ttggagtact accagaagcg acaatttcta tattaaatcc
     8041 attatcagtt agagctttgt atgctttctc catagaagaa tcttcttgaa cacaaacatc
     8101 tggaacacct tctggagaac tacaaccata cacttgacct ggatgcgaac ttataccaac
     8161 aatcttgatg ttttctagct tagaaatttc agtagcaagt tcaaccactt tttctggttc
     8221 aaccccaaaa cggtgtaagc cacaatctac aataataagt agcttgcact ctatttttga
     8281 tttagaaaaa gcttcactat aagccttagc acattctata ttatctgtag cacagattaa
     8341 atttattttt gatgccatag tcacaattct atttatattt acttctccca tgtaaggata
     8401 agccaacata atatcttcaa aacctgcttt tatcagagct tcaccttcat caagtgttcc
     8461 agctagaaaa ccactagcac ctgcattttt ttgaagtctc gctatgtaag tacttttatg
     8521 tgtcttaagc ataggccaaa gttgtttatt attatttgta caaatttgtt gaattttatc
     8581 tatattcttt tctaactggt ctaaatctag caaaaaattt ggagtagcga cttctgaaat
     8641 tttcatgtta tttaccacct ttcaacaaaa gtaaattaac tataagtatt ataattatta
     8701 tattagtaat atcggcttta taatattaat agcttataat tatattatag aactagcgtt
     8761 aacagagagc aatatttatt tgtaataatt agaattttca ataagttagt gtagttttaa
     8821 attatttata taatatattt ttaattttat gcaaagttta tttaaattag tttgaggatt
     8881 tattaatagg gtattattga atgagtaaaa aagtgatgtt tacatagaat aatcacacta
     8941 taaattaatt tagaacctaa aaaattaatt gagcaaattg gaaaagagaa ggataaaaaa
     9001 gatatttgga tagttggtgg aggcgatata gtaaaattat ttatgaaaga taacttaatt
     9061 gatgattatt atatatatat attgccaata atattgggga gagggatttc actatttaag
     9121 tctggtatag acaagactaa tttgaatttt aagaaagcta gtaatattgg agaacttgta
     9181 aaattggaat acagtaaaaa acaaaaataa attacatgcc aatttgttac aatgttatga
     9241 aacgaagcat ttaaaagact tttggctata tgaattgaca ttacttgtca aatatgttaa
     9301 catataagcg acttgtcgga gggtgtatta aaaagaatat gctggagaag agagtgcgtt
     9361 aactcttttc tccttttttt ataaaattaa gcatttataa atttagtatt attacatgat
     9421 tacactatct caaaaattaa gaaaggagaa aaatatgaaa ttaattttaa gttatttaaa
     9481 gaattataag ttattaatag tattaaacat actggctata tttagttttg cattagtcga
     9541 gttggggatt ccaactataa tagctaaaat aattgacaat ggtattgcaa atcagaatat
     9601 agcttacata aaacaaatgg gaatagtgat tgttattatt tctattatag gggttgtagg
     9661 tagtatattg ttgggttatt gttctgctaa ggtatcaaca agtgtaacca gagatataag
     9721 aaatgacata tttgagaaat cacaagaatt ttcacatact gaatacaata aatttggaat
     9781 ttcttcaatg attacaagaa caacgaatga tgcatttcaa attcaacaat ttgttaatat
     9841 actactaagg actgccttat taactccagt tatgttcatt ataagtataa taatgacaat
     9901 tagaaccagt gtggaacttt ctatagtatt ggctatatca gtacctttta taattattgg
     9961 agtagcaata atagcaaaag tatctcaggc aatatcttca aaacaacaaa aaggactaga
    10021 taaattaaac cttatatcga gagaaaattt aactggaata agagtaatta gagcttttgg
    10081 aaatgatgat tatgaaacag agcgatttga aaaaacaaat acatattatg ctaatgtttc
    10141 taagaaacta tttaagctta tgtcaattac tcaaccagca ttctttttac tgttaaatat
    10201 agctgttttg gctgtatttt ggatttcaag tgagaagata aatattggag agcttcaagt
    10261 tggacagttg gttgcatttt tggaatatct tttccatgcc atgttttcaa ttatgctttt
    10321 ttcaatggta tttattatgt atccaagagc agaggtatct gcaaatagaa taaaagaact
    10381 attaaatgaa gaaccattga taaaaaatcc tgaaaatgga attaaggata ctgaaaataa
    10441 gggtatcata gaatttgata atgtaacttt tacctatcca gatggagaag cttctgtact
    10501 taaggatatt tcatttactg caaaaacagg tgaaacagtc gcttttatag gaagtactgg
    10561 tagtgggaaa agtactctaa ttaacttgat acctagattt tatgatgtaa cagaaggtag
    10621 cattaagata aatggagtag atataagaga atatgattta aaagctttaa gaaaaaagat
    10681 aggattcata cctcaaaaat cactgctttt tactggtagc atagcaaata atattagatt
    10741 tgggaaacat aaggcaggag agtcagaatt agaatattcc gcaaaagttg cacaagcata
    10801 tgagtttata tcaaaaaaac ctagaaagtt tgatgaattg attagtgaag gtggagcaaa
    10861 tgtatcagga ggtcaaaaac aaagactttc tattgcaagg gctattataa gaagacctga
    10921 aatatacatt tttgatgata gtttttctgc acttgacttt aaaactgatg caatacttcg
    10981 tgctaaattg aagaaggaaa caaaagatgc aattgtattg attgttgccc agagaattag
    11041 ttctattatt gatgctgata agattatagt actcaatgaa ggacaagttg taggaatggg
    11101 aactcataaa gaattattaa aaaactgtga aatatactat gaaattgcaa catctcagtt
    11161 aaaaaaggag gaattagaat aatgaaaaag tctaaaggtt ttaagaaaac aattggtaga
    11221 cttcttccat ttgtaaagaa atataaattt tcctttatta tagctattat atgtataatt
    11281 tcagcagcta ctatgaatgc acttgcacct aaaactgaag gattgataat aacacaattg
    11341 actaaagatg taattagtat cgcaaaagga gtaccagggg cttccgtaaa ttttgattat
    11401 gtaacaaaaa tattggtgat tttagcatgt atatattttg ccaatgcaat ttttacgtat
    11461 acttcaagtt ttctacttac gaatgctatt caaaacacaa tgagagattt aagaaatgaa
    11521 gtcgaaaata aaataagaag attgcctatt agttattttg atagtaatag ttttggagat
    11581 gtattaagtc gtatatcaaa tgatgtagat acaatatcaa atgcattgca acagagtttt
    11641 atgcaaattg taaactcaat cttagtcata attttggcac tttcaatgat gtttacaatt
    11701 aatatatata tggctcttat agctcttttt ataattccaa ttagttattt tgtatctaaa
    11761 tttgtagtaa aaaaatctca aagtagattt tcattgcaac aaaatgctct tggaaaatta
    11821 aatgggaaag tacaagaaat gtatactgga tttaatgaaa taaaactata tggtaaagaa
    11881 gaagattcta taaaagaatt taaaaaagta aatcaagaac tttgtgaaaa tggatttaag
    11941 gcacaattta tatcaagtat gatgaatcct atggtttcat tagtaactta ctttggaatt
    12001 gcagctgtgg cagttgtcgg ttctatatat gcagtaagtg gaggaataac agtaggaaat
    12061 ttacaagcat ttgtgcgtta tatatggcag ataaatcagc cactttcaca aatgactcag
    12121 ttatctacag taatacaatc ttcttttgca gctatagaac gtgtatttga aattttagac
    12181 gaagaagagg aaatacctga tgttgaaaat ccagttaaga tagaaaatgt aaaaggaaat
    12241 gttacatttg agcatgttaa ttttggatat ggagaaaatt caactttaat agaagattta
    12301 aatgcagaag taaaaagtgg tcagatggta gctattgttg gacctactgg agcaggtaaa
    12361 acaacattaa taaacttatt gatgaggttt tatgatgtaa agaaaggtgc tattaaaatt
    12421 gatggagtag atataagaga tatgaaacgt aaagatttgc gttcaatgtt tggtatggtg
    12481 cttcaagata catggttatt taatggaacc atttttgaaa atatagaata tggtagattt
    12541 ggggctacta aagaagaaat tatacaagca gcaaaagtag caaatgttca tcactttata
    12601 accactcttc cagatggata caatatgttt ttaaatgaag aagcatctaa tatatctcta
    12661 ggggaaaaac agcttttaac catagctaga gcttttatat cagacccttc aatattgata
    12721 cttgatgaag ctacgagttc tgttgatact aggctggaac ttatgcttca aaaagcaatg
    12781 agaaatctta tgaatggtag aactagtttt gttatagcac atagactttc tacaattcgc
    12841 aatgctgact tgattttagt tatgaataat ggaagtatta tagagcaggg gaatcatgat
    12901 gaattaatgg aaaagggtgg attttatgag aagctttata atagccaatt tgcagacaaa
    12961 gaatcagaat agataacata aacttttgta taaaattttg tatcaaattc aaatattaat
    13021 catataatta aaaagagtat cagtaagttg tttttttata gatttactga tactctttga
    13081 ttttaagact aaaggtattt atgattggta cttataattc acaactagtc ttgttacatc
    13141 tgataaccag aattacagta cttttctttt ttaccttttt gttctctaac atataacaat
    13201 tcgcttaact tatcaatgta ataaccatca tcacttatgc gaattaaact agtcagataa
    13261 tatccatctt tgcttctagg aatcttttta tttattgcta aatcaatagc tgtctttgct
    13321 gcttcaaaat gctttaaatg tgtatgacct tcttcaaaac tctttctagt attataaact
    13381 acataacctt ttttaacttg aaaaactata aattctttct tttcatatat tttattaaac
    13441 attcaatcag cccttctcaa gatagtttta tagcataaca ttagaagctt ctaaaaaatt
    13501 ttacaaagaa cataaatatt ttataaatta taaatctaaa actataattt ataaagataa
    13561 ctttctgatt atttatatga taattcatat agattaaaag taaattatct ttaggttaaa
    13621 caaaagtaaa ataaaagact agttataata tttatatgaa aaagtacaaa atatagtata
    13681 tgaatataga aaatttaaat tatttattta cagtttttat ttccataagt atatttacct
    13741 tcagatacct tgtcatcatg gaaatatatt ctttcacaac ttttatctcc ccatagaatg
    13801 ttttttgtta ttttatggga agtatctcct gtaaatactt cagctacagt agtatctgta
    13861 acatcacagt aaagagaagc aaatttttta aaccaaggat tttcctcaaa tcttaaaagc
    13921 caggaagctc cataggggca atgtaactct tcaaattcag gaacccatcc tagaaatgga
    13981 acatcagttg tgctaataat attgtctgca attaattcat ctccattttc atgagctctc
    14041 tctctcatag caatagctct ttcttcagac ctttcaaata aaacttttct taatatttcg
    14101 ctagttttat cttcaccaaa ctcttcaaat aacactttgg aaaaataaaa atataaatca
    14161 ccaaaatgtt tgcatgccat acgaacttct tcaatagcat aatctttgtc aatagtttca
    14221 ttagttacaa catcttcgtt caaaaaaatc actccttaga ataagtatta gttacaatat
    14281 aattatagaa tattttttat aaattaggaa attaatttga gatagatata tttttaataa
    14341 atgaaagtat aatactaaaa taacttattg ttacaaaaat cttctaaatt aatgtattta
    14401 ggagtttcca tatgaatgag agtagttctg taaatatctt caatacatcc atttgcaata
    14461 ttattaaaaa tatctgtgtg ctcttttaat gttgtttcaa gccttccttc atgtgacaat
    14521 gaatcgagag ctaatttttt tattctgtac atataattgt taaacatttt gtcaaattca
    14581 gtattttgag catatgaaac tatagttgtg tgaaataatg tatcgtattc tgcaaatgag
    14641 tttaaatttt tatcacctaa taaaatatct ttttgtttat ctaaaagatg ttttaattta
    14701 cttatagttt ctactgctct aggttctttg tattgacttg taattaaaaa agtacaaaat
    14761 ccttctattg cagagcgtat ttggaatatc tctacaattt catttgctgt aatttgatgg
    14821 attctaaatc ctttgcttgg aattatatct ataaatccat cttgactaag tctttgaaga
    14881 gcatctctta taggtgttct agagatacct atttcactag caagttttgt ttcagaatat
    14941 atttcatttt ctatcatttc tccagatagt atcaattctt ttaagtagtc atatgcttgt
    15001 tcttgtaatc ttttaaatgt agccatactt aataataccc ccctaaacaa tataaaaata
    15061 caaaaactta tataatttta agcatacaac atagtgaaaa taaggtcaat cttttgcaat
    15121 ttaaattgca gataagatta aaaaatataa aaaattattg aagtgtgaag catgattaac
    15181 attaaaggca gattaattaa aactaattga aattagttaa aatacaaaac tgtaaaagta
    15241 gtatattata tttgaaaaca atagtatgta ttaaaactta tataggatga ctaaatataa
    15301 taaatgactt taaaaattac tatttattgt taaaataaat acttttatat tttatattga
    15361 ccggtatact ggtatgtagt atactctaaa acataaaaaa cgttttcaaa aatgtgaaga
    15421 tatggagggt tataaaatga aattgggatt tattggatta ggaataatgg gaaagccaat
    15481 ggcaaaaaat ttattaaaag atggatttaa tcttttggtg tatgacatta ataaatctgc
    15541 tgtggatgaa cttatatcat gtggagcaaa atacgcaagt gttttagaaa tgggtcaaga
    15601 atgtgatatt gtatttacaa tccttccaaa tggaactatt gtgcaggata tattgtttgg
    15661 gatggatgga ttggcaaaaa ctttaaaaga aggaagtata gtagtcgata tgagttcagt
    15721 tactcctaca gaatcaatat tgtgtgcaaa taaattaaaa gatatgggct tagaatttat
    15781 agactcacca gtaagtggag gggagccaaa agcgattgat ggtacacttg cttttatggc
    15841 tggtggtaag gaagaaattt ataagaaagt agagccattt tttaatataa tgggaagctc
    15901 atctatttta attggagata atggtagtgg aagtgttaca aaacttacaa accaagtaat
    15961 agttaatttg acgatagcag cagtttctga ggcatttgta cttgcagcaa aagcaggtgc
    16021 agacccagaa aaagtatata aggcaataag aggaggccta gctggtagta caatattgga
    16081 tgtgaagata ccaatgatta tgaatcgtga ttttaaacca ggtggaaaaa tatctattaa
    16141 tttgaaagat attaagaatg taatgcaaac agcacacaat ttagatgtac cactacctat
    16201 gacaagtcaa ttattagaaa ttatgcagac attaaaagta catggacatt tggaagatga
    16261 ccatagtgga atagcacagt attttgaaaa gttggcaggt gttgaaataa aaaaacacaa
    16321 taattaatat ttaaatatat caatataatt aaatacatat tattaggatt agattatata
    16381 aatacagcga ggttaagtta tcatagtaaa agaaaaataa aattgataaa taataaagta
    16441 gaatgataaa ggggtaaaga ttatgattac aggatatgga cttttgatag catttgttat
    16501 atctatagga attttacttg tatcgattat taaatttaaa gtaaatccat ttttggcatt
    16561 actgataaca tctataatta caggctttat ggttagaatg cctataaatg aaatatcaac
    16621 cacaatatcg actggatttg gaaatacatt aggtagtata ggtattgtaa ttggacttgg
    16681 aataatattt ggaaatattc tttcagaatc aagagcaaca gaatctattg caaaggggtt
    16741 gttagctaag actggagaaa aaaacagtgc tttagcagta actacagcag gttttttaat
    16801 ttcaattcca gtttttatgg atgcagcttt tgttataatg atgcctataa ttaagtatgt
    16861 ttcgagagta actaagaaat ctctaatggt ttttgtatgt gcattaggag ttgggactat
    16921 agtaggtcat gcacttgtaa tacctacacc aggaccttta gcagttgcag caaatgtaaa
    16981 tgcaaatgta ggtagtttta tactttattc tataattgtt gcttttccag cagctcttat
    17041 aggtggatgg atttatggaa aacgttttga aaaatatcca gcttatgcta ttgatgagaa
    17101 tgatagagaa aaaaatttag agcagggaaa agatagcata aaaattgaag atgatgattc
    17161 ttctaaagca ccaggttttg gaatttctat gttttcttta ttgttcccaa tacttcttat
    17221 acttatatca aatgtattta gtatgttctt agaaaaaggt tctacaatga gtggagtact
    17281 tgcatttata ggagataaaa acatagcaat acttcttgga atattggtgg caataggatt
    17341 tttaaagaaa tacataaata aacctatggg agatgtagtt attgaagctg ctgattcagc
    17401 agggctaata ttattgataa caggttcagg tggtgcgttt ggcagtgtta taaatgcaag
    17461 tggtataggt aacttcttag ttgatactat gtctggtctt agtatatcag ttgtagtgct
    17521 tggattctta ttaagtgcct tgcttagaat ttctcaaggt tctgctactg tagccttagt
    17581 aacaacatca tcaattttag gacctacaat tttggcaact ggaatgtcac cagttttagt
    17641 agggcttgct atatgtgctg gaggagttgg tttctccctt ccaaatgatt ctggattctg
    17701 ggtactttca agatttagtg gattatcagt taaagatact ttaaactctt ggacaattgg
    17761 aggaaccatt gctggagttg tagcatttat aatgatttta ttgcttagtg ttataaatgg
    17821 aattatacca cttccaggtc tataaataac aaactattgt ttatatcata taaattacat
    17881 ttagactata caaattacat aaaggggcgt tttaaatgag agaagtaaaa ccaattagca
    17941 tagatatact taatactttt aaacaagtag atgaggacag attaaataaa cttcttgcag
    18001 atgagcttaa gcatttagat agaaaaattg ttgtattaga tgatgaccca acaggtgtac
    18061 aaacagttca tgatatttct gtttatactg attgggataa ggatagtatg gaacaaggtt
    18121 ttaatgagaa aaatagtatg ttttttatac ttaccaattc aaggggattt actgttgctc
    18181 aaactacaaa agcacataaa gaaatttcaa agaatattgt ggatgtatca aagaaagtta
    18241 ataaggattt tataatcata agtcgtagtg attctactat gagaggacat tatcctcttg
    18301 aaactaattt attaaaaagt gaagtagaga gattatcggg gaagttattt cagggtgaaa
    18361 taataatgcc attttttaaa gaaggtggta ggtttactat agataatgta cattatgtta
    18421 aagaaggaga aatacttgta ccagcaggaa tgactgaatt tgcaaaagat aaatcttttg
    18481 gatataaatc atctgatata ggtgagtggt gtgaagaaaa gacaaatgga aagtacaaat
    18541 ctagtgatat gatatatatt tcgtcaaaag aacttagaga gctaaatatt gaagctataa
    18601 cagaaaaact taaaaaagct gaaggattca ataaaattat tgtaaatgca accgattatg
    18661 tagatgtaaa ggtatttaca attgcgttta tacgagcagt aaatagtggt aaggaattta
    18721 tgtttagaag tgctgctgcc ataacaaaag tacttggcgg agtgagtgac aaggaactac
    18781 ttactaagga tgaattagta tcaaaaggta ataccaatgg aggaattata cttgttggtt
    18841 cacatgtgaa taagactacc caacagttag aagaacttaa aaattgtaaa tatccgatag
    18901 aatttataga atttaatcaa catttagttc ttcaagaaaa tggtctaaaa aatgaggtaa
    18961 aaagagttat aaaagttgta gaggaaaaaa taagcagggg taaaactgtt gctgtttata
    19021 ctagacgaga gcgttttgac ttggatacga atgataaaga taaacagctt atgatttctg
    19081 tagaaatatc agatgcagtt accagtataa ttggtatgct taatgtaaga ccaaatttta
    19141 ttatagctaa gggtggaatt acttctagtg atgttggtac taaagcacta agagtaaaaa
    19201 aagctacagt aatgggacag ataaaacctg gtataccagt atggatgaca ggcaaagaaa
    19261 gcaaatttcc taatatgcca tatataattt tccctggaaa tgtgggagag attagcacac
    19321 ttagagaatc agttgagata ttgatggggt agtatattag tgtattgaag tttaaacgct
    19381 taaatttact gagttaaaat taaaataaac taaaattata aattaagatt aaaaataata
    19441 gtatattaat tagggtttag agtattttac aaaaaatatt ctaaacctta atcttatgta
    19501 ttagtatttt tattatttaa cttatgtgtt tattttatta tttatctttt ttaattgatt
    19561 taatattttc tttgatttta tttaaaatat ctgtatgctt attagagctt atgttgggga
    19621 atgctttgat tatacgtcta tgaagtaatt tgtggttaga cagcaaagta ttaagtttat
    19681 tttttagctc atcaatacga tttttagcat agcgataatt ttctttttct ttaagtattt
    19741 caaacctatc ttccaaagta tccagtctat tttcaactaa agattcaagt ttgttttcta
    19801 tagtttcttg tgtaataagt ttgtagtatt ctttcttatc ttttaattcc ttaattataa
    19861 gattgatttg ttttaactta ccatttaatt gtaaaattgt atggactgtg ataactaaat
    19921 ctaaaacaaa ataaatttct atagatatag caaatataaa tagcacattt ttggagatag
    19981 agttaataaa gtcaaccaca ataggatgta ctataagcat tacaatcaat gataatacac
    20041 caaacataag tgagttttcc agacaaactc ttccatgaat attaaattta tgtttagaat
    20101 aatcccacca agtcatatta aatatttttt ctaaaataaa gctcgtacaa tactccatta
    20161 tgctagttat aattatacta aacaaaaata acaaaggaat attgtgtaca aaaggagtca
    20221 aggtagcaac tattgctaat gctccaaatc catatatagg gcaaaatgga ccatttaaaa
    20281 aacctctatt tacaaattgt ttttgtaaaa ctgaacaata tgtagtttca caacaccatc
    20341 ctataaagct ataaattaca aaataaagaa attgaatata aaatgtactc ataagcacct
    20401 catttctagt tagtataaca ctatatactc gatattttat ttttatgatt aatttactaa
    20461 attcaattta catattgatt ataccaattt atagaaattt atcaaattta attacagtta
    20521 gaacattata aacatggtta taataatgaa attttattta aaataaataa gggtgtcaag
    20581 tataatcaaa ctaatagttg tagatactct acactatgtt agtttaatta catttgacac
    20641 ccttatattt atactatgac tatttacatt cgaagctaga gcaacatgtt tcaatataaa
    20701 gatttactct atcagtttca ggagcaacca atatattatc aagtgtacat aagctaccat
    20761 ctttgttgta tttacaattt tttacagtac aaactaattc ctcacaattt ctattattaa
    20821 cgttgctaga taaattacta taaatagatt catctaaaaa tgaactacaa caagtatgtc
    20881 tatcattgtg agctttttca ccatttataa aaattttatc tgcataacat atatggctgt
    20941 tgttatgtga acagttaatt acattacagt ttattctttc caaagttatt acctcctaaa
    21001 cgattattta ttataaatta tgtccatata attacaagaa tataaccatt aagagaaaac
    21061 taccttttag aaaatatcta ttcttagagt aatttgaaat ataaatatat tttcttctaa
    21121 aatttaccta aattgtataa aaacttacag cttggagaaa ataatagaaa aaggaggcgt
    21181 aatcaatgtt tgaaaagaag aaaaaagaat tatttccttg gaaaatatct gccttaatac
    21241 tggttggagt atttgtatta agtagcggag tttatatagg gacaagatta aaacacaatg
    21301 gagttcaaac tggtgaagtt gtaagtacta gtgcgaatat gaatgaaaag tcaaaagaag
    21361 cttttggatt aaataaaaat tgtgaagttt ggcttcaaag tgaaggcgaa aatgaaacta
    21421 actataagag aacacttaca atggcaggaa tagttccaga gtcattatta gataagacga
    21481 agtcagaaat agtatcatat tttaaaaaag aatatccaac taaaaaaata aagagtatgg
    21541 ataaaaatga aatcatattg gtagaatcta aatatagtga taataaagat gtacaagcaa
    21601 tggatgttac tataaaaggt aaatatacta ttgaaaatga taatggaaat atagcactat
    21661 ataaatatga tgatagtgga actaaaacat tagtagaaaa aacaaaaata agagtagatt
    21721 cacttcctaa aacagttcaa gatgaaatca aaaaaggtgt agttatggat actgaagaag
    21781 aggcctattc aagattagaa gactttgcaa gttaatagta aattaatatt attttaagtt
    21841 aaatactaag taagttaaat actaagtaaa ttaataataa ataatttaat attaaataga
    21901 ttagtattaa aattgtagtc acatttaatt cctataaaaa tacaataatt tatttgtaat
    21961 aaatataaaa gttatattga ggtaaaatct tgctttgata taactttttt atttgatttt
    22021 atattttaca tagagcattt tatgatttgt taatattatt tatgatataa tgatttagaa
    22081 cataagtttt tatgagaaat acattttggt atttctttta tttttagggg gaattttaaa
    22141 aaatgataat attggggatt gacccaggta tagccatagt tggatatggt ataattgaat
    22201 acaaaaatag caagtttaaa gtaatcgatt atggagcagt tacaacacct gcccatatga
    22261 atatatcgag aagattggaa cttgtgtata aaggaattga tacaatagta aagaattaca
    22321 atatagatga agttggaatg gaagaattat tctttaacaa gaatgtaaaa acagctataa
    22381 cagttgcaca agctagaggt gttactatgc ttgcatgtgc tcataatggg aagcctgtat
    22441 atgaatacac tccacttcaa gtaaaacaag gtgtagttgg atatgggaga gcagataagg
    22501 cacaagttca acagatggta acttcatttt taagtctaaa aaaagttcca aaaccagatg
    22561 atgttgcaga tgctttagct gtggctattt gccatgctca ttcaaacaaa cttgaaaaaa
    22621 ctctaaagaa tataggtggt aagtatgtat agttatataa aaggtacagt agaagaaatt
    22681 tatatagaca gtatagttgt tgaaaataat gggataggat ataaaataaa tgtatcatcc
    22741 aatacaataa tgaatttaca agtgggagaa gccacaaaaa tatatactaa attaattgta
    22801 agagaagatg atatgagtct ttgtggtttt gtaagtaggg aagaacttaa gatgtttgaa
    22861 ttactaacat ctgtatctaa aataggacct aaggtggcgc tttcaatact atcatttgca
    22921 tcaccagcac agttaggagc atatatttta agtgaagaca ttggaaaatt atccaaagct
    22981 cctggtgttg gtaaaaagac agcagaaaga atcgtcttag agcttaagga taaggtagac
    23041 aaaaataaca tagagtttga accaacgtta ttatcacaaa aacctacctt gatatcacaa
    23101 gatgagtctg tagatgcact tgtagcttta ggctatactt tgtcggaatc aaaagaggca
    23161 gttcaaaaat gtaaaaaaga tggtatgaat acagaagcta ttataaagaa agcacttaca
    23221 tatataatga gtaaatcttt gaaatagtat ttggatttta taatatttgg attttaaaac
    23281 aatatttaag gagtttatgt aaatttgaaa atatataaag tagattttca aaagtatgag
    23341 aggaggggta taaatgcaag gttttgaaga tgaaaatagg ataataactt ctacaatgaa
    23401 gatggaagat atagatatag aaaacagttt aagaccaaaa actttagaag attatttggg
    23461 tcaagaaaaa tccaaagaac aacttagtat atttatagaa gcagctaaat cgagaaatga
    23521 gcagttagac catgtgcttt tatatggacc accaggtctt ggaaaaacta cactagcaag
    23581 tataatagca aatgagatgg gggtaaattt aagaattacg tcaggacccg caatagaaag
    23641 agctggagat ttggcagcca tacttacgaa tttaaatgaa aatgatgttt tatttataga
    23701 tgagattcat agaatcaata gaagtgttga agaagtttta tatccagcaa tggaggattt
    23761 ttgcctagat ataataattg ggaaagggcc atctgctaga agcataagac ttgaccttcc
    23821 taaatttaca cttattggag ctactacaag agctggtatg cttacaaatc cacttagaga
    23881 tagatttggt gttatttgta aattggacta ttatacagtt gatgaacttt caaaaatagt
    23941 acttaggtct tcaagcatct tagatgcaga gatacagtct aatggagcat tagaacttgc
    24001 aaaacgttca agagggactc caaggatagc aaatagatta ctaaaaagag ttagagattt
    24061 tgcacaagtt agagctgatg gaaaaataac tgataaggtg gcaaaagatg ctctggaact
    24121 tttaggagtc gatagtttgg gtctcgattt tgtagatgag aagcttctta tgactataat
    24181 agaaaaattt agaggaggac cagtgggttt agatacattg gcagcttcta tcggagaaga
    24241 tagaaataca atagaagatg tatatgagcc atatttgctt caattaggat ttataaatag
    24301 aggtcctaga ggaagagtag ccatgccatt agcatatgaa cacttaaaaa ttccatatcc
    24361 taatgaaaaa taaaaataga ttatacgagt atttataaaa taaaagagtc attaaattaa
    24421 aatatttcta aaatggctaa aaattcaaac atatccacaa aatggtaatt atatctgtag
    24481 atactgtgga taaacacgat aaaaattgtg tgtattgtat ctatatattg tggataagac
    24541 atgtatatcg tggaaaaata tctatatatt gtggatatgg tgtctgttaa aaatagcaat
    24601 ttcttgatag attatggtaa tattgtggaa aataagttgt ttttttgata ttattgtagc
    24661 tggagaaatt ttaagagagg aagtttaaca ttgaaaacaa gtgattttaa atttgactta
    24721 ccacaggaac ttatagctca agttcctata gaagatagag ctagctcaag attgatggta
    24781 ttagataaag agacaggtaa tattgaacat aaagtattta gagatataat agaatattta
    24841 aatccaggag attgcttagt attaaataat actagagtta taccagctag attaatagga
    24901 gagaagcttg aaactggagg taaaatagag tttttattat taaaaagaac agaggaagat
    24961 acttggcagg ctttagtaaa accaggaaaa agagcaaaag tagggactaa attttctttt
    25021 ggaaatggaa agctaattgg agaagttgta gatttatcag atgaaggttc aagaattata
    25081 aaattccatt atgatggaat ttttgaagaa atattggatg aacttggaaa tatgccacta
    25141 cctccatata tcactgctag actagatgaa aaagaaagat accagactgt ttattcaaag
    25201 cataatggtt cagcagcagc acctacagca ggattacatt ttacagaaga attgttaaat
    25261 aaaataaaag aaaaaggtgt agatatagcc tttgtaactc tacatgttgg tttaggaact
    25321 tttagaccag taaaagtaga ggatgtttta aatcacaaga tgcattcaga atattatatg
    25381 gttagccaag aagcagctga caaaataaat agagcaaagg aaaatggaaa aaatgtaata
    25441 tgtgttggta ctaccagttg tagaactata gaatcagctt gtaatgagga tggaaagatg
    25501 aaagaaacta gtggatggac agaaatattt atatatccag gttataaatt taaagtttta
    25561 gataaactta ttactaattt tcacttacca gagtctacac taataatgtt agtaagtgct
    25621 atttgtggaa aggataatgt tttaaatgca tataatgagg cagtaaaaga aagatataga
    25681 ttcttcagtt ttggagatgc tatgattata aagtagggag gtaatggatg catagtttac
    25741 aaaaacaaaa aaagaataac cctatattaa agattgcttt tatatttgaa cgtgttttag
    25801 cagtagtagt tctaatagca gtatttttag gaactataga tgttctaaga cttatgtggg
    25861 gagcatatat agttgatttt caaaatccag tacaatacag tcagcttaat gattttttag
    25921 cacaaatatt acttttagta ataggtgttg aacttgtagt aatgctatct cttcatatac
    25981 caggagcatt tattgaggca ttactttacg caatagctag aaaaatgttg ctactgccta
    26041 aaaatgaagg tatgatagat gttttaatag gagttatagc tatagcagga ctatttgcta
    26101 taaaaaagtt tttagtaaca aaagacaaga gtgctcttaa tataatgagt attcatgatg
    26161 aagaagcaat taaggaacaa cagaacaaag aagacgaaga taaatctatt tagacaataa
    26221 attatatgaa tagcttatta caaatatatc ttaagaaata atgagaaata aatatgatta
    26281 aggagaactt tatgtacgca gtaagatacg aattgataaa gacttgtaag cagagtggtg
    26341 caaggttagg aagattacac actccacatg ggattattga aacaccaata ttcatgccag
    26401 ttggaactca agcaactgta aaatctatga caccagaaga attaaaagag attggttcac
    26461 aaataatact tagtaacaca taccatttat atatgagacc aggtcatgaa cttataaaaa
    26521 gagctggtgg tcttcataag tttatgaatt gggataaacc aatacttaca gatagtggag
    26581 ggttccaggt atttagtcta gggcctttaa gaaagataaa agaagaaggt gtagaattta
    26641 ggtcacactt agatggttca aaacattttc taactccaga aaaagctatg gaaatacaaa
    26701 atgcattggg ttcagacata atgatggcat ttgatgagtg tgcaccatac ccatcagata
    26761 gagaatatgt aaaaaattct ttagaaagaa caacaagatg gttaaaaaga tgtaaagatg
    26821 cccataataa taccgacaag caagctttat ttggtataat acaaggtggg atgtataaag
    26881 acttaaggga acaatcagcg aaagaaataa ctaatattga tttaccagga tatgctattg
    26941 gtggacttag tgttggagaa ccaaaaccac ttatgtatga cgtgttagag catacaactc
    27001 cacttatgcc aaaggataaa ccaagatacc taatgggagt tggtagtcca gatgacttag
    27061 tagaaggtgt tataagagga gtagacatgt ttgattgtgt cctacctact cgtatcgcta
    27121 gaaatggaac tgcaatgact agccaaggta aagtagtagt tagaaatgca acttatgctg
    27181 aagactttac tccacttgac cctgaatgtg attgttatgc ttgtaaaaac tattcaagag
    27241 cttatataag acaccttata aaagcaaatg aaatactggg tgcaagatta ataactactc
    27301 ataatcttca ctttttatta aatttaatga aacaaataag acaagcgata atggaagata
    27361 gattacttga ttttagaaat gaattttttg caaaatatgg atatgaaata taggttttgc
    27421 actattaatt ataatcattt taatatataa tattcaatag tactcaaaga tagagaaagt
    27481 aaattgttat ataaacattt attatgattt acaaaagagt ataatttaca aaggagtgaa
    27541 aaattaatgc cacagcaaca aataattatg agtataggtc tatgggtagt agtaatcgca
    27601 atcttttatt tcctaatgat tagacctcaa aagaaaaaag acaaacaatt aaaagaaatg
    27661 agaagtagcc taagtgtagg agacaaggtc attacaatag gtggaatagt agctaatgta
    27721 gctaaagttg aagatgatgt tgtaatatta gaattaggac caaatagaac taaagttcca
    27781 tttgaaaaat gggctatagg aactgttaaa tctaaaaaag aagaaataga agaagactaa
    27841 attagtaatt taaaagcctt ttctcgaata tatattaatt tgagaggagg tttttttatg
    27901 ggaaaatcta attatatttt taaaggtcta ggatatgcat atataataac tttggctgtt
    27961 ttattagtgt ataacttatt tttgacattt acagatattg gtggagataa tataactatg
    28021 gtctcatcat ttataactac tatttctgca gctataggag ggttttacac ttcaaaacac
    28081 atgaaagaaa aaggacttat gtatgggctt ttagtaggac ttttgtacat agtatgtatt
    28141 tttttgactg tgtttttagc acaagaaaag tttgtatttg aaatagggat gatttataag
    28201 ttattgctaa tatcagcagc aggaggcatt ggaggcgtat tgggtgtcaa ctttaaatag
    28261 agtttatatt aaagtagttt tatgatataa tcaataggaa acaattagaa atggaggcgt
    28321 tgaaaatgga aaataaacat gtaaaaactt tatctcaagc tactttaaag caaagtgctg
    28381 caaaaggtgg atgtggagag tgtcaaactt cttgtcaatc agcttgtaaa acttcttgta
    28441 cagttgcaaa tcaagagtgt gaaagataga aaattattga tttagttgtt tgtaagaggg
    28501 tatctcaaag tgattctttt atcattatga ggtatcctct tttattttca attattatat
    28561 aaaaatatat attctattta tgtaaaatta aatttgattt cttatcgttt aattatgcaa
    28621 aaatat
//
LOCUS       DAENCV010000046        26456 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAENCV010000046 DAENCV010000000
VERSION     DAENCV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.046"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            131..898
                     /locus_tag="KQD82_001967"
     CDS             131..898
                     /locus_tag="KQD82_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380437.1"
                     /translation="MEEILYVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            885..2822
                     /locus_tag="KQD82_001968"
     CDS             885..2822
                     /locus_tag="KQD82_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380438.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLITISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            3047..4660
                     /gene="ggt"
                     /locus_tag="KQD82_001969"
     CDS             3047..4660
                     /gene="ggt"
                     /locus_tag="KQD82_001969"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG4380439.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGSVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(4793..5644)
                     /locus_tag="KQD82_001970"
     CDS             complement(4793..5644)
                     /locus_tag="KQD82_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG4380440.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            6270..7163
                     /locus_tag="KQD82_001971"
     CDS             6270..7163
                     /locus_tag="KQD82_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG4380441.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            7163..8557
                     /gene="gltA"
                     /locus_tag="KQD82_001972"
     CDS             7163..8557
                     /gene="gltA"
                     /locus_tag="KQD82_001972"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG4380442.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            8923..11142
                     /locus_tag="KQD82_001973"
     CDS             8923..11142
                     /locus_tag="KQD82_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4380443.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            11289..12035
                     /locus_tag="KQD82_001974"
     CDS             11289..12035
                     /locus_tag="KQD82_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380444.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGESIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            12035..13915
                     /locus_tag="KQD82_001975"
     CDS             12035..13915
                     /locus_tag="KQD82_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380445.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            14034..15425
                     /locus_tag="KQD82_001976"
     CDS             14034..15425
                     /locus_tag="KQD82_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4380446.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            15532..16572
                     /locus_tag="KQD82_001977"
     CDS             15532..16572
                     /locus_tag="KQD82_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG4380447.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     IEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            17061..17714
                     /locus_tag="KQD82_001978"
     CDS             17061..17714
                     /locus_tag="KQD82_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG4380448.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFISKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            17790..17999
                     /locus_tag="KQD82_001979"
     CDS             17790..17999
                     /locus_tag="KQD82_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380449.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(18139..18705)
                     /locus_tag="KQD82_001980"
     CDS             complement(18139..18705)
                     /locus_tag="KQD82_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG4380450.1"
                     /translation="MMNNELNTIILETLSNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            18866..19519
                     /locus_tag="KQD82_001981"
     CDS             18866..19519
                     /locus_tag="KQD82_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG4380451.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(19677..19853)
                     /locus_tag="KQD82_001982"
     CDS             complement(19677..19853)
                     /locus_tag="KQD82_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380452.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
     gene            20381..21325
                     /locus_tag="KQD82_001983"
     CDS             20381..21325
                     /locus_tag="KQD82_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG4380453.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            21347..21973
                     /locus_tag="KQD82_001984"
     CDS             21347..21973
                     /locus_tag="KQD82_001984"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG4380454.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            22007..23056
                     /gene="hisC"
                     /locus_tag="KQD82_001985"
     CDS             22007..23056
                     /gene="hisC"
                     /locus_tag="KQD82_001985"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG4380455.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            23059..23637
                     /gene="hisB"
                     /locus_tag="KQD82_001986"
     CDS             23059..23637
                     /gene="hisB"
                     /locus_tag="KQD82_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG4380456.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            23639..24244
                     /gene="hisH"
                     /locus_tag="KQD82_001987"
     CDS             23639..24244
                     /gene="hisH"
                     /locus_tag="KQD82_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG4380457.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            24241..24963
                     /gene="hisA"
                     /locus_tag="KQD82_001988"
     CDS             24241..24963
                     /gene="hisA"
                     /locus_tag="KQD82_001988"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG4380458.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETALNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            24945..25703
                     /gene="hisF"
                     /locus_tag="KQD82_001989"
     CDS             24945..25703
                     /gene="hisF"
                     /locus_tag="KQD82_001989"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG4380459.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            25731..26399
                     /locus_tag="KQD82_001990"
     CDS             25731..26399
                     /locus_tag="KQD82_001990"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG4380460.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT        10479 a         2584 c         4564 g         8829 t
ORIGIN      
        1 atataattaa tacttacgaa aatgtaacct taaattaata taattaaatg tttaaaagcc
       61 attttaatat atataatata tttgtgagaa ttgataaata actaattgtg atgaaaggtg
      121 attattaact atggaagaaa tattatatgt tgaaaatata aaaaaggagt atggaagaaa
      181 aggttcaaaa catgaagctc taagaggaat aactttcaag gtttataaag gagaatttgt
      241 tggtattatg gggtcttccg gtgctggtaa atcaacactt ttaaatatta tttctacaat
      301 agatttacct tcatcaggag atatttatat aaatggaaag aatactatta aaatgaagca
      361 aaatgaattg gcagatttta gacgtgataa tctaggcttt gtatttcaag attctaatct
      421 tttggataca cttacaataa aagaaaatat aatgttgcct ctttctttaa aaaatgaaag
      481 agtatcagta atagaaaata gaataaaaga gatttcaaaa gaattaaata tagaatctat
      541 attagataaa taccctggcg aagtttctgg tggacaaaag caaagaggag ctgtatgtag
      601 agctatagca acaaaaccat cattagtact tgcagatgag cctacaggag cattagattc
      661 taaatctgct agggatttgt tgaattgtct attaaaatta aacaaagaca gcaataaaac
      721 aatattgatg gtaactcatg atgctataag tgcgagtttt tgcaatagaa tactctttat
      781 aaaggatgga ataatattca cagaaattgt aaagggagaa agtaacagag agttctataa
      841 taaaatagta aatacagttt ccttaattgg aggagttaat aaaaatgact ttatttagaa
      901 ttgcaatgca aaatgtaaag aatagttttt ttaattattt aatgtacttt atttcaattg
      961 tatttagtgt atttgtattt tttagtttta aatctataga gtataatgaa gcgttgagtt
     1021 cacttggaga gaaaactagg atgagtatta atacaagttc aattgtaata gtggtatttg
     1081 tatttttatt tatatattat tcaaactcat tttttttcaa tagacgtagg cgagaagtag
     1141 gaacttatag tttacttgga atgaggaaaa atcagatagg aaaaatattt ttatatgaaa
     1201 cttttttgat gggtatagtt gcaatattaa taggtatatt tttaggattg ttattttcaa
     1261 agcttatgac tatgatgctt gtaaaattaa tgaatgaaat gatagtagta aatatgaact
     1321 taagtataaa ggccttagtt cagacattag ctgtattttt aataatattt atagttattg
     1381 gaataagaaa tactatagtt atcagaaata agagaattat agagttattt aataaatatc
     1441 cagaaaagta taagtttaaa aaatttataa agttaaaatg tgtacttggt gttttattaa
     1501 taactatttc ttatttaatg tcaattagtt attttattgc tgaaaatatt atgctttcag
     1561 tttttatatt agttacaata atacctggaa cttttttatt tttttcatca actatgtata
     1621 tgattattga tgctgttaag aagagaaaaa gtttttatta taagggacag aatttaattg
     1681 cattttctga acttggattt aaattaaaga gcaatagtag tgtattggct gtaatagcaa
     1741 tattaatagc tacaagtgta actatgctag ggtttaccat atcactttat tacgatatag
     1801 ataggaacat aagtgaaaat tataaataca gttataccat aaatttagga aattcttatg
     1861 taaataacga aattgacaaa ttattatata aatataaaaa agataataag ataatctttg
     1921 ataaaacaat agaacttata gacaaagata taaatattga aatgtattat aaaaatggaa
     1981 atagttatat agaagataga acgtctgtag atattattag agaaagtgat tttaaaaaat
     2041 taagacaata tcagaataac aattatgaag aattaacttc aaaggaatct gtgtactatg
     2101 tttctgattc atacaaaaaa atatttttta aagactttga gattgaaaaa ataaacttgc
     2161 atattaaaga tacaaatgag tatgatataa atctgtttaa agtacaaaaa agcatcttgg
     2221 agccagaaat aaattcacaa gctacgtttg atttaattgt agtaaaagat gacgtattta
     2281 atcagttaaa attacatgga aattctagat taataagatt aattgatata aaaaatgaaa
     2341 aaaaagaact ggatttgact ttaaaattaa aaaatatagt aagtgaaaat atgaaattta
     2401 cttatccttt taattttaca tcaagtattg aaagttataa taacttgata aaattaagtg
     2461 gtctaatgct gtttataggc atatttttgt ctgtagtatt tcttttatgt acaggtagta
     2521 taatcttatt taaacaatta tcaaatatat atgatgataa ggaaagatat attatgttaa
     2581 taaaacttgg agcaaataat aaagatattg agaaaataat atcaaaacag ttaaaagtta
     2641 tatttttaat gccattagtt gtaggtacag ttcataattt atttgctatg tctatagcac
     2701 aaaagtttat accaaggtca ttacttgtac caattatcat aactctagtc atttatttta
     2761 taggatattt tatatattat tttttaactt taaaatatgc tctaaacatg ataaaagaat
     2821 aaattatttt taagtcaaaa gaattagata tatgttttga ataaaataac ttatttaagt
     2881 gcagatagaa aatactagaa ttgagtttaa gatatttatt taaagataaa attaaagtta
     2941 ggaaaaagtc tatattttat atcattttgt atcagttaca ttacttgatg ttatataata
     3001 aatctaagca gaatatcaaa taaaaaatga cgaggagctg aaatttatga agtttaatgc
     3061 ctaccaacat aaatactcat caataagaaa tgtcgtgtat gctaaaaatg gagcagttgc
     3121 aacatcaaca ccattagcat ctcaagctgg acttgaaata ctaaaaaagg gtggaaatgc
     3181 tgttgatgca gcagttgcaa cagccgccac attagcagta gtagaaccta caagcaatgg
     3241 gattggagga gatgcatatg cccttgtttg gatagaggaa gaaaaacgat tgtatggttt
     3301 aaattctagt ggctttgctc cagaaaatat ggaattaaaa aattataata atatgaaaga
     3361 aatgccaaaa tatgggtttg gatcagtaac tgtacctgga atacccgcag cttggtcaga
     3421 attaaataaa aaatatggta aacttagctt aatggaatgc ttatctccag cgataaacta
     3481 tgcaagagag ggatacgtag tatctcctaa tgtagctaag gtatggaaaa aatcatatga
     3541 actctatgaa aaagaactta ttggagaaga gttcaaacca tggtttgata cattttctaa
     3601 agatggtaaa gctccagagg ctggagatat atttatatgt gaagaacagg caagtacatt
     3661 agaagagata gcaaatactc aggctgaaag cttttataga ggaagacttg ctgataaaat
     3721 agatgaatat tctaaaaaat ttaatggggc tataagaaag agtgacttag agtgttttta
     3781 tccaacatgg gtagaaccta taagtacaga atataaggga tataaaatat ttgaaatccc
     3841 accaaatggt catggaataa cagttttaat ggcacttaac atattaaaag aattggaatt
     3901 agaaggaaat atagaaaatg tagaagacat acataaaatt atagagagtt taaaattagc
     3961 ttttgcagat tctaaaacat atgtaactga tatagagcat atgaaggtaa agattcagga
     4021 attactaagc caagagtatg ccaaaaaaag aagtttatta attgacaata aagaggcttt
     4081 gtatccaact gcaggagagc catattgtgg tggtacagta tacctttgta ctgcagataa
     4141 ggatggaaat atggtttcat atatacagag caattacata aattttggtt caggaattgt
     4201 gataccaaga actgggattg ctttacatag tagaggaaat aattttaatt tagaccctaa
     4261 acaccacaat gtagtaaaac catttaaaaa accttatcat actataatac ctggattttt
     4321 aggtaaagaa gataaagcaa taggtccatt tggtgtaatg ggagctttta tgcaaccaca
     4381 aggacatatt caggtgttaa caaatatgat tgattttgga ttaaatccac aagaggcgct
     4441 agatgctcca agatggcagt ggataaaagg aaaagaaata gaagttgaac cagagatgcc
     4501 aaagcatata atagattcat taattgaaaa gggtcatgaa ataaaagtaa ttcatgatac
     4561 tgttgacatg ggacgaggtc aaataatatt taaaacagaa caggaaagtt atatttgtgg
     4621 gacggaatca agatgtgatg gtcatattgc agtttattaa tattatcaac taaaatatat
     4681 ataataaatt taaagagtat atgaaataat aagtaaaggc gaaagtctac aatgactgac
     4741 gcctttacaa aatttaagct atcatatgct gattttatat agattatgag ccttattctt
     4801 gaccagtttt gatttcattg tcatcataac taatccagtc gctaaagcta ccaatataaa
     4861 tcttgtgagg tatatccagt tctctaagag caagactgtt aacacaagca gatatccctg
     4921 aaccacaaga taaaattact tcatcataat tatttaaatc tttaaagaat ttttttaaaa
     4981 attctatatc tttaagtgag ccattttcaa agtcactttt tattaaatcc ttacaaaaat
     5041 aattttttgc acttggtata tgaccagctt ttgagtaagc aggttcaact aaaccttggt
     5101 atctctcatt agcacggcaa tctattatta cagtatcttt tttgtataat ttagacttaa
     5161 catattccat tggaacaact aaaccattgt ttatatttgg tctaatttcc ttacctgtac
     5221 aacttggtat attcactttc tcttccaatt ctccaccttc ctttacgaaa gatgttattc
     5281 ccccatctag tacatagaca tttttaagcc ccaaatgttt taattggaaa aataatctgc
     5341 aagctccatt taaatctcca tcatcataag ttacaatcgt agtgtcatta tcaacaccca
     5401 ttttttctaa tttttctttt agtattaaag ggtcttgtaa aggattttta ccaccatgtt
     5461 cttttgtagg agatgataaa tccttgtcta tatctaagat aaaactgcct ttgatgtgtc
     5521 cttttttata agaatcaata ccataagttc tattgataag atcaaatctg caatctatta
     5581 caactagatt atcatttttt tctagttttg atataagttc ttttgctgaa ataatgtttt
     5641 tcaaagaatg tacctcctgt gtttatatta tctttatttt aatttatatt aattattaac
     5701 tttataaact aatattatta taatttatta cataagtaaa taaatcttta gaaattgtaa
     5761 aaattttata taaaaattca aataattaat gaatattgaa aattttagat atataataaa
     5821 ataagagtat attttataca atatgaaagc tggtattgca atagattaaa gccttataca
     5881 cgtttagttt ttaaagaaat tagtgttatt atgaaaacct aagttaattt attaaataaa
     5941 taattgaata aaaaaatttt attatgtatt tataaaattt ttgttacaaa tttggcataa
     6001 tatagtatac tttaagtgtg tattaggtta aatctataac aaattagacg tatgatatag
     6061 acttagtgtt tattggcatt tacaagaaat ttgttaatat caactatttg tagtttgggc
     6121 agttataatt attatagggg ggatgtccaa tacaaaacaa tcaaccaaga actaatatta
     6181 tattataaag agttatagtt ttgtgtgttt atgtttttaa ttaaatttaa agtgggaaaa
     6241 atatatttgt tagaatggag aatagcgaga tgagtaataa aatagttagt aagagacaat
     6301 taacagatag tatttattta atggaaatag aggcacctag agttgcaaaa tcttctcaac
     6361 caggtcaatt tataataatt aaaaatgatg aaaaaggaga gaggattcct cttacaatag
     6421 ctgactatga tagagaaaaa ggaacagtaa ctattgtatt ccaaacagtt ggtgcttcaa
     6481 ctaaaaagtt agcaatgttt gaagaaaacg attttgtaat ggattttgta gggccattag
     6541 gacaagcaag tgaatttata catgaagata tagaagaatt aagaaataaa aagatattat
     6601 ttgtagctgg tggagttggt tctgctccag tttatccaca agttaaatgg tttaaagagc
     6661 atggattaga tgtagatgta ataattggtg caagaactaa agaattaatt atattagaag
     6721 atgatatgaa aaaagtagct aaaaatgtat atgtatcaac agatgatggt acatatggat
     6781 ttaatggaag agttacagac ctattaaaag atttagttga taatcaaggt aaaaaatacg
     6841 accaagcaat agtaattgga ccaatgataa tgatgaaatt tatgtgccaa ttaactaaag
     6901 aactaaatat acctactata gtaagtttaa atacaataat gatagatgga acaggtatgt
     6961 gtggtggctg tagagttagt gttggaaatg aaactaagtt tgcatgtgta gatggtcctg
     7021 aatttgatgg tcatttagtt gattttgatc aagcaatgag aagacagtct atgtacaaga
     7081 ctcaagaggg aagagctatg ctaaaacttg aagaaggcga tagtcatcat catagtaatt
     7141 gtggatgtgg aggtaacaaa taatggatgc taaaaaagta aaagtaccag ttagagaaca
     7201 agaacctgct gtgagagcaa caaattttga tgaggtatgc ttagggtata ataaagaaga
     7261 agctatggca gaagctaata gatgtttggc ttgtaaaaaa ccaaaatgtg tgggtggatg
     7321 tccagtagga atcgacatac cagggtttat aacaaaaatt aaagaagatg atatagaagg
     7381 tgctgctaaa gtaatagcta agagcagttc attaccagct gtttgtggta gagtatgccc
     7441 acaagaaagt caatgtgaag gtgtatgtat acttggaata aaatcagatg ctgtatcaat
     7501 aggtaaatta gaaagatttg tagcagattg gtcaaaagaa aatgacataa atttatctga
     7561 tacagagcct aagaaaaatc aaaaagtagc agtaatagga agtggtccag caggtctagc
     7621 ttgtgctggt gatttagcta aaaaaggcta tgatgtaaca atctttgaag caatgcatga
     7681 gccaggtgga gttttaactt acggaatacc tgagtttaga cttccaaaac aagctgttgt
     7741 tcaacctgaa atagacaata taagaaaatt aggtgtaaaa atagaaacta atgtaattgt
     7801 tggtaaaaca attacagtag atgaacttat agaagatgaa ggatttgaag ctatattcat
     7861 aggttcagga gctggtcttc caatgtttat gaatatacca ggggaaaatg caaatggagt
     7921 attttctgca aatgagtttt taactagagt aaacttaatg aaggcttata gagatgatta
     7981 tgatacacct attagttctg gtaaaaaagt tgctgtagta ggtggtggaa atgttgctat
     8041 ggacgctgct agaactgcat taagattagg ttcagaatca tatatagttt atagaagaag
     8101 tgaaaaagag cttccagcaa gagctgaaga agttcatcat gcaaaagaag aaggcattat
     8161 atttaataca ttaacaaatc ctaaggaaat tttagtagat gaaaatggat atgttaaagg
     8221 tatggtttgt ataagaatgg aattaggaga gccagatgat tctggaagaa gaagacctat
     8281 agaaatagaa ggttctgaat ttgtacttga tgtagacaca gttataatgt cacttggaac
     8341 aagcccaaat ccactaatat cttctacaac aaagagtctt gatataaata agaagagatg
     8401 cttaataaca gatgaaaatg gtcagacttc taaagagggt gtatttgcag gtggagatgc
     8461 tgttactgga gcagcaacag taatatctgc aatgggtgct ggtaaaacag cagcagcatc
     8521 aatagatgaa tatttaaaag caaaagttaa tgcttaattt gtaaattaaa gagagggtgt
     8581 ctcaaaatga actttttagt tcatgaggca ctctttttgt acaaaatcaa atatgtttta
     8641 atcatttcaa caatgaaaat ttcattcctg tagaaagtct aacttttagg tcttagtaca
     8701 atctctattt tgatgtagcc acttttttag atggtttaat aatttatcag ttattattgt
     8761 aatatttgat actaagtaac ataactttgt ctaactatga taaaatagtt atacaataaa
     8821 ggtcttgtaa ggagtgaata atgtgtggct aataaaatca gatttgtcaa atctaacatt
     8881 aaataataga aaaataaatt gtgtaattaa tggtatttta atatgaaaaa aagagaaaaa
     8941 gtatttgatg ggctagtttt taagcatata ttaatcttat taatactatt agttttgatt
     9001 gatttaacaa gtaccatcat atatgcaagt aactctatgg aaattacttc tagaaatatg
     9061 attgaggcat caaaaagaga acttgaaaac tatttagaag taaacatatc tttattaaaa
     9121 gctcttagtc aagatgatag attttctaat ggtgaaacat cactaattga aaagggaaaa
     9181 ttattaagtc catatcaaaa ggaatataat ctttttatga ttggtataac agatacaaaa
     9241 ggaaatgctt caagtacata tagggaaaat gttaactcaa taaaagataa gcctagtttt
     9301 gagaaagcaa ttaaaacaaa gcaagtagta gtttcagata ttgaagtcag taatgtaaca
     9361 ggtgacaaag tatttatcat atatgtacca attgtaaaaa ataatgagat gattgggacc
     9421 atttttgctt ctttttattt tcaagatgta aataatttaa taagtagaag caattttgat
     9481 gattctatta aatttctaat gatagataaa gattatacta ttatttctca tccaaacaaa
     9541 aaatatgtaa atgataaaag taagatgctt gatttagaag ggaatattat aggaaccact
     9601 aaaagtgaaa tcttaaaaaa tataaataaa aaatgtcaag gtgatttttt atcttgggat
     9661 aattggagat tatataatgt aaaatatacg aacataaagt ggactaattg gactttagta
     9721 tcaaaatgta atatatttaa gaactttaaa aatctaatag ttaattttat gataaagttg
     9781 tatttttata tagtaatatt tatgatattg tggaaattaa gtaatgctaa attaatagaa
     9841 caacttaaaa aattggctta ttatgattct ttaagtggaa taaaaaataa agaaaagttt
     9901 agaaaagatt ctatgtatat tttaaagaat tattatcaag ataattttta cttagtacaa
     9961 ttggatgtca ataaatttaa atatataaat gagatgtttg gatatgctga agggaataag
    10021 atattaattc atatatcaca agttttaaat aataatacaa ataaatatga aatttgtgct
    10081 aggatggata atgaccattt tatattgttg attgcttgta acactgaaga tgaactttta
    10141 aatagattat ctaaaataaa taaagaaatt tgtaacttaa atactactaa ctcatctaag
    10201 tataaaattg ttatgtcaag tggaatatat aagattacta aaaaggatga tattaaaaag
    10261 attgacttgt taattgatag agcaaatatt gcagcaaaaa gtaaaaagga aaaatatgaa
    10321 cattcatatt cattttttaa tgaagataca agaaatcgat tgtacaagga aaaacgattg
    10381 gaagataata tgaataaagc tttagaaaaa ggtgaattta tagtatatta tcaacctaag
    10441 tatagtttag atgatgtaaa tgaaattgaa ggagcagaag ccttgataag atggaatagt
    10501 ccagaatttg ggtttatttc acctatagat tttgtacctt tatttgaaaa gaatggtttt
    10561 attgtaaata ttgatatgtt cgtgtttgaa gaagtatgca aaacattaaa caagtggata
    10621 aataaagggt atactcctgt accaatctct gtaaatatgt ctagagttca tttatatagg
    10681 gataatttta tagaaaatat aactgatttg ataagtaaat acaatatatc tcctgagttt
    10741 attgagcttg aactaacaga aagtgttgtg tttgataatt taaatatatt gattgatata
    10801 atgaaaaaga ttaaagagat aggattcctt atatcaatgg atgattttgg ttcgggatat
    10861 tcatctttaa atctgttaaa agacctttct tttgatattt taaaattaga tagaggattt
    10921 ttaatcgaaa caacagatac aaaacgtgga aaaattatta tttctaagat tgtggaaatg
    10981 gcaaaagcga ttgatattaa agtaatctgt gagggcgtag aaacttatga acaagtagag
    11041 ttcttgaaag agataggttg tgataaggtt caaggatact tatttgctaa acctatggta
    11101 ttagatgaat ttgaaaaaca cttaaacttt aaatttgatt aatcattaat aattattaga
    11161 ctataagata ccctaacaac ttacttacaa atatgtaagt taattgttag ggtattttta
    11221 tggttaaagt agtatattca cgatattatg gtattgtagt aaaaataaaa aattatggag
    11281 aatataaaat gaacatattg agtattagaa atatatcaaa aacatattgc ggaaatattc
    11341 catttaaagc attagataag gttagtctaa atatagaaaa gggagaattt gtgtctgtta
    11401 tgggaccatc tggaagtggt aaatctactc ttttaaatat aatatctaca gttgatagac
    11461 agagtgaagg tgaggttgta ttagatggat atgatgtaag taaattgaaa ggagaaaaac
    11521 ttgctgaatt tagaaggaaa caacttggtt ttgtatttca ggattttaat ttgattgaca
    11581 ctttaactgt tggcgaaaat attatgttgc cacttacact agagggggaa agtatcaaag
    11641 atatgaatat acaaactaaa agtatatcta aatttttagg aatagataaa attttagata
    11701 gaaaaaccta tgaaatttca ggaggtcaag ctcaaaggtg tgctattgct agagctatta
    11761 taaataaacc tgccatactt cttgcagatg agccaactgg aaatcttgat tcaaaatcta
    11821 ctgatgatgt tttgaaatta tttactagga taaacaaaga acaaaaggta acaacactta
    11881 tggtaactca tgaagcatat agtgcaagtt attcagatag agtaattttt ataaaagatg
    11941 gttgtatata tacagaaata aaaaaatctg aaagcagtaa ttctttctat tctgatatac
    12001 tagctgtttt gtctcaaata ggaggtgtaa gatagtggat tttagaaagt ttgcttataa
    12061 taatatagtt agaaatttca gggcatattt agcatatttt ttaagttctg ttttttctat
    12121 aatgatattt tttgtttttg cagtgtctat gttccatcca attataacaa aatctggaat
    12181 gcagagtggt tctactgcat ttatggctct tatgacttca gaattaataa tattcggttt
    12241 tagtctactt tttatattgt actctttagg aaatttctta aaatatagat tgaaagattt
    12301 tggagtattg atgataattg gaatgagtaa cagacaactg aaaaaattga tattaattga
    12361 aaatttgata ataggatttt tagcagtgat gttaggaata ttacttggtt tatcaatttc
    12421 aaaattgttt ttattgtatt taagcaaact gttttatatg aatttaactg aattttattt
    12481 tccagtaaaa gcaatgctat tgactattgt atcatttatg attttatttt tgataacagg
    12541 accaatgagc ttaaaattat tgagtaaaaa gagcatttta gagcttttag taggtacaaa
    12601 aaagcctaaa aaagagataa aatcttcaaa gctatttgga ataattggtg taatatgtct
    12661 tatattagga tatgctatga tactatttgg caataagtta aaaataaatg gaggtatgta
    12721 tacaacagca ctattaatta ctttggggat atttttattt ttttctcaat ttactatttt
    12781 gatattaaag tatttaaaaa ataaagaaaa attttataag aacaaagtaa atatgttact
    12841 tataagtaat ttgatatata aattaaagga taatacacga ttgttatttt taataacaat
    12901 acttttatcg gctactttag tagcatttac aacaacttca acacttgtaa gttctcaagg
    12961 agaagcttct aaaaataatt tcccaatggt ttactcatat ttttcagagc ataataatga
    13021 aaaagaatat gaagaactac aacaaattag agaagcaatc aaaggatatg attataagga
    13081 gttaagtttt cctgtactta aatataatag ggaagtctta ctaagtgtaa agaattataa
    13141 caaattatca aaggaattgg gattagataa aattagttca aagttagaaa aatatgaagg
    13201 gattataatt cctagtagca attcgcaaga acatataaat tctttaaaac aattaaaaaa
    13261 ttatagtata aaaaatttaa atatagaagt aaaaaaatca aaaaataaaa taatttttcc
    13321 tacaggactg tttagtaaag ttgtagttat aagtgatgac ttatacaatg aagtaaaagg
    13381 tgaattttca aaaattaatt ttcatggatt tgattataaa aactggcaaa gcagtgcaca
    13441 gataactgaa aatttaaaag aaaaacattt taatttggat agagaatata acaggtcata
    13501 ttttttaaca ttaccagata tgtatttagt agagttacaa caaagtaaaa ttcttcaatt
    13561 tatgggtgtt tttataggag tgatattatt tatagcagtt tttagttttt tatactttag
    13621 attatataca gacgagatag aagataatgt aaagtacaaa aaactatcaa aaataggtct
    13681 atcttttaaa aatatgaata agattgttag tatagagata ggtacattgt tttttatacc
    13741 gtattttata gcaattataa atagcataat ttctttactg tttttaaatc aagtattaaa
    13801 taatagtttt aagttagaaa atttgggtat attactactg atatcatcta tatattcact
    13861 ttatttttac ttattaaaaa aaatttatac tagaaaagtt tggttttttg attaaagtgg
    13921 tatataatag atatataaat taatatttaa aaagtagtta atattagggg agggcatttg
    13981 cacctccctt gtaatatgtg gaaaattgaa tattagaaga aggaggagac ttgatggaaa
    14041 atcaacaatt attaggaaca gaacgaatta gtaaattact actaaaatat tctataccag
    14101 ctataatagg tatgttggta aatagtttat ataatgtagt agatagaatt tttattggaa
    14161 atataccagg ggttggacca cttgcaataa caggtcttgg tgtaacaatg ccaataatga
    14221 caataatact tgcatttggg atgttgatag gtataggaac tactactacc atatcaataa
    14281 aacttgggca aggtaaagta gaagaggcaa gaaaacttat aggaaatgca atgacgttat
    14341 ctgtaataac aggaattatc ataatgatac ttggaatatt atttgcaaat aaaatactta
    14401 cattgtttgg agcaagtgaa aatactttaa tatatgcaaa atcatatata aatattatat
    14461 tattaggtac agtagtaaat cttctttcct tttctttaaa ccactcaata agagctgatg
    14521 gtagtcctaa aatttcagca ggaataatga tagtaggatg tttaactaat atagttttag
    14581 actggatatt aatatttgga ttcaatttgg gaatacaagg agctgcaatt gctacagtaa
    14641 cttctcaggc tcttacagct attttaacta tagggtatta tataagtgga aaatcaaact
    14701 tacgattcag taaatctaat ctaaaattag ataaaaagct aataaaagca gtgtttgcaa
    14761 taggtatgtc gccatttgct atgcaacttg cagcaagttt agttcaagta atatcaaata
    14821 ttgcacttaa aactcatggt ggagatttag ctataggggc tatggctact atttcttcta
    14881 ttgcaatggt atttttgatg cctatctttg gaataaatca aggagcgcaa cctattatag
    14941 gtttcaatta tggagctgaa aaatatgata gggttaaaaa agcatattta ggttcattag
    15001 tagtagctac tataatttta tgtatgggta tggtagtagt tatgcttttc ccagaagcta
    15061 ttataggtat atttaataaa gaccctgaac ttatgaatat atctgttaat ggattaagaa
    15121 tatacttact tatgttgcca attgtaggct tatcagttac aggaacaaac tttatacaat
    15181 ctataggaaa agcaaaaatg gctatgttat tgagcctttt aagacaagta attttattga
    15241 taccagcagt attaattctt cctacattct taggcttaca gggtgtatgg acagctcaac
    15301 cagtgtctga ttttatagct acagtaatta caggtatagt tgtatttaga gaattaaaaa
    15361 gatatactcc taaaactgaa aaattaaatg aaaatgaaag actaaacgag ataactactg
    15421 agtaaaaata taaattcaga taattgagca agaaattgca aaaaatacga ttatatgata
    15481 aaataaaacc gctgtcaagg ataagagaaa ttctaattat ggagtgattt tatggaattt
    15541 acaaagaaaa gcaaattaaa aaatatgtat gtaaacatga atgtagctag aatgttgtct
    15601 aagataaatg agtataaggg tagacaactg ctttacaaaa aacaaccaaa agaaatttta
    15661 gaaaacttag aaaaaaaatc tttagtagat tgttctgaat ctacatacat tggaaatcaa
    15721 agagatagta gtaattttaa tctagaaaag ttgataagta atgaagttac acctagaagt
    15781 agagaggaat tgagtattgt tgaatacaga gatgtggtaa aaactattaa cagtgcttat
    15841 gagagtattc ccatcagttc acaaacgatt cttgagcttc atggatactt atataaattt
    15901 tcatctacta gaggaggaag ttataagtca gataatgatt ttattgaaca caatttaaaa
    15961 cctagcgaat ttataagtac tgattcaagt gtcaataaag ttgaaaaagc tatagaagaa
    16021 atatgtgaag cttataatac attgatagag gaagatgaaa ttgatatatt aatattaatt
    16081 tctgcatttg tgttagattt catattgata catccattca aagaaggtaa tataaaaatg
    16141 gcgagagtac ttatcctatt gttactaaat aaaaatgggt atgaagttgg aagatatata
    16201 agtttaggaa agatatttga tgatagttca tatgaatatt atagtaattt aaattatttg
    16261 aaagcctcaa taggtagtga aaaagcagat atgaatgcat ggattgagta ttttttagag
    16321 acgatattaa ctgcatatga gaagctggat gatagtttaa atatatctga taagaaaaga
    16381 caaacaaaaa ctagtagaat agagaaaata ataaattcta cccttggata ttttacaaaa
    16441 gaagatataa gagatttatg tcctgacatt ccagaaccta ctataaatag agtttttaat
    16501 aatctaagaa aacaagataa gatagaagtt gttgcaagag gaagaagtgc aaaatggaag
    16561 aaaaaatatt aaaaaatgtt tagataacta aaatggtata gtgttttaca ctataccatt
    16621 tttaatgtca ttagcatgag caataattta acaacttaag taatatcaat tttatttatt
    16681 gattattttt gaactgaaaa atggcgttat atggtatacc tcagtggtaa ttaattttgg
    16741 atgagaagat ataatattac agaatatatt aggagtatat gtagaaaaaa tataataaaa
    16801 gtgtgaggct tagtaattaa caaattatgt ggcttcacac ttttttataa aataaagata
    16861 cttagcagaa taatatttta gttgatatgt ttataagatg gtacttgttt gggtaaaagt
    16921 ataaatagta aaaaagtaga agtaatgtgt ataaaaacat aagttacatt ctgtaataat
    16981 agttatttta taattcaaca aaagaataga atgggtatat ttgtgaaata ataatataaa
    17041 ataatcttag gagtgagctt atgaagaaaa aattattata tataaatgta aattcaaaac
    17101 ctgaagactt atcttcaagt aaaactgtag caagaaaatt tataagtaaa tttatggaaa
    17161 aaaataaaga ttttgaggtt gaagagatag acctttataa agaacatata ccaagacttg
    17221 agtatcagta ctttgaaaaa agaaatagca tagttagtga agaaaatgct aaaaatttag
    17281 acgataagga tagaaaagaa cttacaaaga ttagaaatct ttgtgaccaa tttgtgagtg
    17341 caagtgtata tgtaatagca gcacctatgt ggagtttatc ttttccagca ccacttaaag
    17401 agtatataga ttgtataatt caagatggaa aaactatatc ttttgaaggc aatgataaac
    17461 cacaaggaat tttaaatgat attgatagaa gtatggtata tatacaatct tctggtggac
    17521 acataccatg ggtgttgaag ccagttatga ataaagggtt aaattatgtg gaaagtatta
    17581 tgaaatttat aggaattaaa aaatttgatg aattgttagt agatggaact ggaactagtg
    17641 aagaagaaag acaagctgca atagaaaaag catctgaaaa aatagatggt ataatagatg
    17701 ggatgaaatt ttaattataa tattttatta aaaatataaa ggaaaaacct cttttaatgt
    17761 agaaacttat attgaaggag gcgagaatca tgcgagaaga aaaaagtaat gaaaagtatg
    17821 attgttattg gtgtaatcaa gagaataatt tctgtgtaga aataaaagat aatatagtca
    17881 tgatagatga tggcactggt acgttaaaac aagccgtttt catagggtat aaacaaatcc
    17941 aaattaattt aaattgttca cattgtcaaa acttaaatag aataaaatta aatttgtaga
    18001 attttaaaat ggaaattatt gaaggaatta aaaatagaaa gtgttaatat ttagactgaa
    18061 aattatattt tatacaataa aagtttattt aaataacatt gtattttaat atttattttc
    18121 agtcttactt tattaaaatt attttttggt tatgtctgga aaatatctgt ctactaattt
    18181 ttctgaaata ttttttaatt ggtcagccat tgaagtcaga cttaataagg ctatcaataa
    18241 atcatcttta ttttctggat ttatactaat attttcaaaa ttagactcta taaagtccgg
    18301 tatattcttt ctttgatttt cttttattaa catgaatttt tcgtaacatg aaactaaatc
    18361 tttttcactg aaattatcct tttcatacac accatgtaag attcttttaa tttcttgtat
    18421 tgtaagtgta tttttcatag aataaataat tatcatctgt aaaatttgtt ctttagtata
    18481 cttttttccc ttaactggtt tgattaatcc ctctttgctg taattattta tcatggtttt
    18541 agtaagaatt ttatcacttt caaatctttt attagcagaa agcttattgt caattagaga
    18601 aataatttga tccatatata agtcaataga tggaatatcg tttgaagtta tatctgcatt
    18661 acttagtgtt tctagtatta tagtattaag ttcattattc atcatatgtt acctccataa
    18721 tatatataat acactaaatt agaaagtaaa acaatattaa ggcttgtaaa aaaaacataa
    18781 agtggtataa ttaaatatat aatatagtaa tggaaactat atttgtgaat ctgattttta
    18841 aaatacataa ttgtaggagg acgaaatgta tcaatttttt ttaaagggta gagacccaat
    18901 aagcagttta actcatttta taggtgcttg tttatctctt ttagcaacta taattttagt
    18961 ttttcaatct gttacattac aggaaacttc tctcttgatg atagtatctg tatctgtatt
    19021 tggactttca ctaattgctt tatatagtgc aagttcgtat tatcattttt taaaggggac
    19081 accagaacaa gaattgtttt ttagaaaagt agaccatgca atgatttacg tattgattgc
    19141 tggctcttat acaccaatat gtctgaattt tatggaaaaa aaagaaggca taatatttgt
    19201 tacagcaata tggatagttg catttattgg cataataata aaaatatttt ggatggatgc
    19261 acctagatgg ttatctacca gtatatattt acttatgggg tgggcaattg tttttgatat
    19321 aaatgcattc aattcaattc ctaaggattg ccttagacta ttaattatgg aaggtgtatc
    19381 ttactctata ggtgcgatta tttatataat taaaaagcct aatataagtc ctgagtttgg
    19441 atttcatgaa atatttcata tttttattat gataggttct ttatttcact ttttagcagt
    19501 tttattgtat gttttatagt tttaaaagat ttgattaatt gatactaaaa ttctacaatg
    19561 aataaaatgg aactgcctta tatagattat atcttagaca ataagacagt tccttattat
    19621 aaatattaaa ttctacttat aataaaattt tgataaaaat agtccattca aaatgcctat
    19681 tccataattc ctttaatgta cttttcattt acgacatata gtttgaattt atgtactata
    19741 attatatcct ctaaattctc tgaaacacta acaattctga cacatttgtt tctaatttct
    19801 acaggtaaat ctaaatcatt tgttaaattc acatagtcat catatttata cataaaatca
    19861 tccctttaca ttaagatata cttattatac tatattttaa taagtttttt taaattatta
    19921 caatttttcc cgaaatttca ttttttatac ataaaatttc caaattaggt aaagattaaa
    19981 ttttttattg tataaacaaa agttacatat aaaatatttt attcaatttt ttaaataact
    20041 attgccaatt tttgaaaaat atattattat taatagtaac aaaaaactgt aaatttagaa
    20101 tattaataaa aattatttgg gaggaaatta tcatggtgag attaaatata gtaaaattaa
    20161 atacaaaatt aaatcatcac catcatcata gattaaaggg attgtaatat gaaattaatt
    20221 catagacaca agccctgttt ttagtgtaat tggtcttgta tctatgatgg gaagtaattt
    20281 taagagaaaa ttatagccat ataggtataa agcctatatg gcttttttat tgttttaaat
    20341 ttgcaaatat aagagtaaat ttaatgaaga gggtgagaat gtgaaatttt tgaggattga
    20401 agatgaggtt atttattcta aaaaaagata taaattaaaa agggaaatag ataaactttt
    20461 tttggatgaa gaatattttc aaatagaacc acaattattt gaagaatatg atgaatttac
    20521 aactataaat aataaaattc cagaagaatc tatggtaaag gttgtaaatg gaaaagtaat
    20581 ggtacttaga gctgatataa ctacaagtat aataaaaagc cttgtaccaa gatgggaaga
    20641 tggtttaaag cttaagcttt tttataattc atcaatatat aaaaataaaa atacagttgg
    20701 aataaaagag ataagacaaa taggttgtga gtatctagga gaagcctctg tagaagcaga
    20761 tagagaagta gtgaaattag ctcttaagat acttgaaaaa tacaataata attttattct
    20821 agaggttggg agtagcaagt atattcatgg tttgttagaa gaattaaatt taaataagaa
    20881 ttgtgaaaat caaattaaaa atttactata tacgaagaac actcatgaac taaaagtata
    20941 tattgaagaa ttaaaaatta agcgtgaagt aaaagagtta ttatcaaata ttttaagttt
    21001 acaagggaat ttatataatg ttattgaaaa atcaaacaag ttctattgta ataataagat
    21061 gaagcaagct cttgaagaat taaagcaagt aaataattta attgaggaat gtaatttttt
    21121 agataaggct agatttgatt tatcaatgat tactatgctt gactattatg aaggaattat
    21181 gtttagagga tactacccaa actcatataa agaaattctt agtggcggaa gatatgattc
    21241 tttaactaaa gagtatggaa aagaaattcc agccattgga tttacactaa gtgttgatga
    21301 gttaatgaag tatgtatata agtaaagttt ataaggaggg attgttttgg attatttgac
    21361 tatagcacta gcaaaaggac gaattgaagg ggaatcattt aaaaaattta aaaaaatggg
    21421 tctaggagat agtatagaca cagatacgag aaaactaatt tttaaagatg aagaaaataa
    21481 aattatatat atacatgtaa agccatcaga tgtagttaca tatgttgaaa aaggagtcgc
    21541 tgacctcggt atagcaggta aagatactat tttagaaaat gagacagatg tatatgaaat
    21601 atatgattta ggttttggaa agtgtaaatt tgctgtagca ggattaaagg gagatagcat
    21661 atatagagaa gatgaatatt taaaagtggc aacaaagtat cccaatatag caaaaaagta
    21721 ttttaaagag aaaggtcaaa aaattgaaat aataaaatta aatggttctg tagaactagc
    21781 tccaatagta gggttgtcag atgtaattgt agatatagtt gagactggca atacacttaa
    21841 agcaaatggt cttgaaatat tagaagacat atgtaatata agtgctagaa ttatatcaaa
    21901 tagagctagc tatagattta aatatgaaca aattcagaat ataattcgtt tatttgaaga
    21961 acttgataat taaaatataa ttgagcttta taaggaggga tttaagttga gagaaaaaga
    22021 aagcataaga gagttaaggg gatatgaacc aaatcacgta aactgtaaag taaaacttga
    22081 tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt aaagaaattt cagatagtga
    22141 tatagattta aatttatatc ctgaagattc ttatagtgat ttgaaagaat ctataataga
    22201 ttacataaat atttctggag taaataagaa aaatcttcta gtaggaaacg gttcaagtga
    22261 aataattgat ttaataatac atacttttgt agataaagat gaagtaatat tgagtttctc
    22321 tccaagtttt agtatgtatt ctatatatag tcagataaat gggtctaaat ttataggtgt
    22381 tgaaagtgat gaaaatttag ttataaatat agataatgtg attgaaaaag taaaggagaa
    22441 taatcctaaa atagtaattg tatgtaatcc taataatcca acgggaacaa tattaaagag
    22501 ggaagagata ataaagcttt tagattcaac taatagtcta gtggttttag atgaagcata
    22561 tatggatttt ggagaagaaa gcatgttaag tgatgttttt aagtatgata atctaatagt
    22621 tcttagaact ttatcaaagg catttggatt ggctggaata agaacaggat atatgttatc
    22681 taatagtagt ttaataaatt ctgttgagaa agtaagacct ccatataatt taaattcatt
    22741 atcagacttt attgctacaa gagcacttag aaataaagat gtggtaaagg cttatataaa
    22801 ggaagtaaaa gaggaaagag aagttttata tgaagaaatg attggtatgg gaattaaagc
    22861 atacaaatca caagcaaatt ttatattatt ttattctgaa atcgaaaatt tatcacaaaa
    22921 acttattgat agaggtgttt taataagaaa atttggtgga aagttagaaa attattacag
    22981 agttacaatt ggtgataaag aagaaaattc tatgtttgtt ggagctataa gagatatttt
    23041 gaaaaaggag aaataggtat gagaatttgg aaggtagaaa gaaatactct tgaaacacag
    23101 attttagtag aattaaatat tgatggttct ggtaaagctg aaatagatac tggaatagga
    23161 tttttagacc atatgcttac cttaatgtca tttcatggca agttcgattt aaaagtgatt
    23221 tgtaagggtg atacatatgt agatgaccac catagtgtag aagatatagg tatagctata
    23281 ggtgaagcat ttaaaaatgc actaggtgac aaaaaaggaa tacgaagata ttctaatatc
    23341 tatataccta tggatgaatc tctatcaatg gtagcaatag atataagcaa tagaccttat
    23401 cttgtattta atgctaaatt tgatacccaa atgattggta gtatgagtac tcaatgcttt
    23461 aaagaatttt ttagagcttt tgtaaatgaa tcgagagtaa cattacatat caatctctta
    23521 tatggggaaa atgaccatca taagatagaa agtattttta aagcatttgc aagagcttta
    23581 aaagagggta gtgaaatagt ttctaatgag atagcatctt caaagggggt tttgtagaat
    23641 gaatataata gtagactatg gtcttggaaa tatagattct gtgtctaggg gttttagaaa
    23701 agctggaata gaaactaaaa tttctagtga tatagatgaa ataaaacagg cagattcact
    23761 tatacttcca ggtgttggag cttttagaga ttctataagt gccttagata aactaggatt
    23821 gataccaata ataaaggagc atgtttctaa gggtaaattt atgataggta tatgtctagg
    23881 aatgcaatta ctttatgaaa aaagttatga gtatggagaa tatgaaggat taggacttat
    23941 aaaaggaagt attgataaac tggatataag tttaaaagtg ccacatatgg gatggaataa
    24001 tctaaaattt aataaggcaa atgatgatat acttaaatat ataaatgaag atgattatgt
    24061 atacttcgtt cactcatatt atgcaaattc atcaaatgaa gaactaattg cattttcaga
    24121 atatgaaaag aaaatccctg ctattgttag aaaaggcaat gtatatggta tacaatttca
    24181 tcctgaaaaa agtggagaag tagggttaaa tatattaaga gcatatgggg agatgataaa
    24241 atgataatat ttccagcaat agatataaaa gataataagt gtgtaagact cacacaagga
    24301 gaatttgaca aagtaaatgt atattatgat aatccattag aagtagcata taaatggaaa
    24361 aatgaaggag cagaatatat acatatagta gatttaaatg gtgctagaag tgaatttggt
    24421 gtaaatacaa aaataattga ggacatagca aacaatatag atatacctat tcaagttggc
    24481 ggtggagtta gggataagga aaaagtcaaa agtttaataa atgctggtgt tactagagta
    24541 atattaggca gtatagcgat tgaaaatttg aatttagttg aagaattggt caatgaatat
    24601 aaagagaaaa ttgtagtttc tatagatgct aaagatggaa aagtagcagt taggggatgg
    24661 gaagttgtaa gcaatgtaga ctcattaaca ttatgtaaac aacttgaaaa gataggtgta
    24721 caaactattg tttatacaga tatatcaaaa gatgggatgt tgcaaggtcc aaattttgac
    24781 atatatgaaa gaatagcaaa ggaaacagca ctaaatgtta ttgcttctgg aggtgtaact
    24841 tctatagaag acgtaaaaag actaaaagct atgaacttat atggtgcaat aataggtaaa
    24901 gcgctttatg ataaaaagat agactttaag gaggcacaac aattatgctt actaggagaa
    24961 taataccttg tctagatgtg agaaatggaa gagttgtaaa ggggaaaaaa tttaaagata
    25021 tagtagatgt tgatagtcca gaagtacttg gaaaatttta tagtgattgt ggtgcagatg
    25081 aacttgtatt ctatgatata actgcatcaa atgaagagag aaagacatct ttagaatttg
    25141 tgacaaaagt tgcagaaaat ataaatatac cattttgtgt tggtggagga gtaaataaat
    25201 tagaagattt cactgatatt ttaagaaaag gtgctgataa agtttctata aattcatcag
    25261 cagtcaaaaa tcctgaatta ataagggaag cttcattaaa atttggagca caatgtgttg
    25321 ttttatcgat agatgcaaag aaaaatgaag agggttcatg gagcgtatac gtaaaaggtg
    25381 gaagagaaaa aactaatctg gatgcaattg aatgggctgt taaaggtgta gaacttggag
    25441 caggagaaat cgttgtaaat agtatggatg aagatggaat gaaaaatgga tatgatatag
    25501 aattattatc aaagataact tctttagtaa atgtacctgt catagcatct ggaggcgctg
    25561 gaaaaaagga agatttttat gaggcggtta ataaatccaa tgtagatgga attttagcag
    25621 catctgtttt tcattttggc gagataaaaa taaacgattt aaagaaatat ttaaaagaca
    25681 tgggtgtaga agtaagatta taagttaaat actaaaagga gattagaaat atggataata
    25741 agtgtaacaa tgtatacagt gatgaagtag agaaatttat aagaagtata aagtttgatg
    25801 ataaagggtt agtccctgtt gttgttcaag aggtagttag caaagatgta ctgatgttag
    25861 catatatgaa taaagatgct ataaagaaaa ccttaaaaga taaagtagct tgctatttta
    25921 gtagaagtag acaagaactt tgggttaaag gtgagacatc tggtaatact caaaaagtag
    25981 ttaagatgtc ttatgattgt gatgtagata ctatactact ttttgttgaa caaactggtg
    26041 tagcttgtca tacaggcaat tattcttgtt tttacagaga cttgtttgat gatagttcaa
    26101 aaatggagct tgaagtacaa acaaatatat tgaaagaatt atatgattta atcaatgaaa
    26161 gaaaaaataa tccagttgaa ggttcttata ctaattatct ttttgaaaaa ggaatagata
    26221 agattcttaa aaaagtaggt gaagagagta gtgaggtaat tatagcatca aaaaatactg
    26281 ataaaagtga acttatttac gaaattagcg atttagtata tcatacattg gttttaatga
    26341 tagaaaaagg tgttgaaatt gatgaaataa aaaaagaatt acttaaaaga agaaaataga
    26401 ataagataga ttaatgggta ataagaaaat ataccacttt taataaaaag tggtat
//
LOCUS       DAENCV010000047        25996 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAENCV010000047 DAENCV010000000
VERSION     DAENCV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.047"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(85..1470)
                     /locus_tag="KQD82_001991"
     CDS             complement(85..1470)
                     /locus_tag="KQD82_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4380461.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKY
                     TVGSFEYTPII"
     gene            complement(1643..2386)
                     /locus_tag="KQD82_001992"
     CDS             complement(1643..2386)
                     /locus_tag="KQD82_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG4380462.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(2406..3668)
                     /locus_tag="KQD82_001993"
     CDS             complement(2406..3668)
                     /locus_tag="KQD82_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380463.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEISEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKGLEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(3852..4850)
                     /locus_tag="KQD82_001994"
     CDS             complement(3852..4850)
                     /locus_tag="KQD82_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG4380464.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWGGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(4834..6345)
                     /locus_tag="KQD82_001995"
     CDS             complement(4834..6345)
                     /locus_tag="KQD82_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG4380465.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVESLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKDILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(6399..7361)
                     /locus_tag="KQD82_001996"
     CDS             complement(6399..7361)
                     /locus_tag="KQD82_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG4380466.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(7409..8299)
                     /gene="rbsK"
                     /locus_tag="KQD82_001997"
     CDS             complement(7409..8299)
                     /gene="rbsK"
                     /locus_tag="KQD82_001997"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG4380467.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(8341..9354)
                     /locus_tag="KQD82_001998"
     CDS             complement(8341..9354)
                     /locus_tag="KQD82_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG4380468.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(9583..10560)
                     /gene="bioB"
                     /locus_tag="KQD82_001999"
     CDS             complement(9583..10560)
                     /gene="bioB"
                     /locus_tag="KQD82_001999"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG4380469.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(10766..11029)
                     /locus_tag="KQD82_002000"
     CDS             complement(10766..11029)
                     /locus_tag="KQD82_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380470.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(11099..12172)
                     /locus_tag="KQD82_002001"
     CDS             complement(11099..12172)
                     /locus_tag="KQD82_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG4380471.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(12420..13178)
                     /locus_tag="KQD82_002002"
     CDS             complement(12420..13178)
                     /locus_tag="KQD82_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380472.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(13175..14092)
                     /locus_tag="KQD82_002003"
     CDS             complement(13175..14092)
                     /locus_tag="KQD82_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380473.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(14092..14292)
                     /locus_tag="KQD82_002004"
     CDS             complement(14092..14292)
                     /locus_tag="KQD82_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG4380474.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(14297..15391)
                     /locus_tag="KQD82_002005"
     CDS             complement(14297..15391)
                     /locus_tag="KQD82_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380475.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(15901..16041)
                     /locus_tag="KQD82_002006"
     CDS             complement(15901..16041)
                     /locus_tag="KQD82_002006"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG4380476.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(16351..17658)
                     /locus_tag="KQD82_002007"
     CDS             complement(16351..17658)
                     /locus_tag="KQD82_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380477.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(17723..18202)
                     /locus_tag="KQD82_002008"
     CDS             complement(17723..18202)
                     /locus_tag="KQD82_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG4380478.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(18249..19112)
                     /locus_tag="KQD82_002009"
     CDS             complement(18249..19112)
                     /locus_tag="KQD82_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG4380479.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(19112..19867)
                     /locus_tag="KQD82_002010"
     CDS             complement(19112..19867)
                     /locus_tag="KQD82_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380480.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(19894..20382)
                     /locus_tag="KQD82_002011"
     CDS             complement(19894..20382)
                     /locus_tag="KQD82_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380481.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(20400..20801)
                     /locus_tag="KQD82_002012"
     CDS             complement(20400..20801)
                     /locus_tag="KQD82_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG4380482.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(20804..21301)
                     /locus_tag="KQD82_002013"
     CDS             complement(20804..21301)
                     /locus_tag="KQD82_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380483.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(21325..21729)
                     /locus_tag="KQD82_002014"
     CDS             complement(21325..21729)
                     /locus_tag="KQD82_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380484.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(21959..24526)
                     /locus_tag="KQD82_002015"
     CDS             complement(21959..24526)
                     /locus_tag="KQD82_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380485.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            24692..25435
                     /locus_tag="KQD82_002016"
     CDS             24692..25435
                     /locus_tag="KQD82_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG4380486.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         8170 a         4903 c         2689 g        10234 t
ORIGIN      
        1 ttagaaagtt aagttaaaaa taatgttaat ttatatattt atcctaattc atacaattca
       61 atcttacttg tattaaatta ctcttcaaat aattggagta tattcaaaac tacctacagt
      121 atacttcaat tctttttttg ctttatttat aaccaattca tcaagcatta catcatttat
      181 tgaacatgat atagatttag atgccaataa cattgcttta aatcccatat cagaacctgc
      241 acttgcacaa gattgccatg tatgggcaga aataccaata ggccaaactg aacaagaaca
      301 ctgtgctgta ggaattatgt aactaacatc tccaatatcc aatgataatg atatacagtt
      361 attatgagta aatgtatcat ctgtaacctc atcatgtaaa ttcatcttag taatatcctt
      421 atttccactt aatacactag aaacactact tcttttatct atagacactg tctcagctaa
      481 tttatctgca aaatagtagt catcttcatt atagttttgt acacctacta atttcatatt
      541 ttgtgataga atatctgtta atattgtatt tggaatatag tcatatactc cacttataat
      601 gtcatattcc ataatggtac cagtcattaa tgctgctcct tttgctatat caacaatcct
      661 accaaataca tctcttacat gctccatttt aactcctctt atgtaatacc acacttctgc
      721 aaaatcagga actatatttg gcatttttcc accattagta gttacatagt gtactctaca
      781 attatcaaat atatgttctc ttaaataatt acaacctata ttcataagtt ctactgcatc
      841 aagtgcactt cttccgttat aaggagcttg tgctgcatga gcactaatcc cattatatct
      901 aaatttcaca gagtaattag caagtgttgg gattcttata ggagtattta tatcaaatgg
      961 atgccaacta aaagcacaat caattccatc aaaaaaaccc ttttttatca tcaatacttt
     1021 tccagataaa tcttcctctg ctggacatcc atagtatttt atagtccctt tcagcttttt
     1081 tagtttgatt aattccttta ctgcaattac agctcctatt gaacctgtac caagtaagtt
     1141 atgtccacat ccatgacctg caccaccatc tacaataggt tctttagtag tggatacttt
     1201 ttgagacaaa ccatctaaag catcatattc tcccaaaact gctataactg gtctaccttc
     1261 tccataacta gccacaaatg ctgtatccat accttctaat ccctctatac taaaaccatg
     1321 tttttttaaa cattcttttt gtaaatttga agcatagtat tctttatact gaagctctgg
     1381 atttttccat atcttatctg atatatctct taattctccc tgtatctcat ctatatattc
     1441 catcaaaaac tttttattta aattatccat attaattata taaacctccc tgtttctaat
     1501 aacttataga ttatcagcca aaatgactaa ttttaactga tttaaaaata ttttctacca
     1561 tacaaccatt ttatacaata aaacctcttg acttgtaagt ttcattttat caagtaagag
     1621 gttttcaagt tttataaaat ttttagcatg ttaaccaatc aagcatatta acccataatt
     1681 tattatagta tttccaatca gtaaattctt ttggacccca gtgaggtgca cagtcagaac
     1741 taaagatagc agattttcct tttccaaact cgccaacagc tacaaatggg tctcctccaa
     1801 tagttgctaa aacaggacaa ttatctctag ctactgtttt gttatatcct aaaaactttg
     1861 gccattctgt tggtatacct tttaatactg gatgttcaga gtcaataact tctggaatta
     1921 ttcctgctgg tttttcaact ctatcatctt tatctatcat tgttattggt aatacatctt
     1981 tgatagcagt ttctccgaat ctagcttttg catctattcc tgtaaatgac atatatccac
     2041 ctaccatgat taacgcccca ccattattta catactcttt gattagttca agtctatctg
     2101 gattactatt gcaatctata aaagtactat tagataataa gaacgtatta gaacctatat
     2161 cagataatat tatacagtca tactcattta actcttctaa tttatacgga aaactatctg
     2221 ctgctacatg tgcaggcata tgaactgtct catatcctcc agattctatt gcttctttca
     2281 accactttac agcttcttca taatgagttg taacaaacgt atcaaatcct tttatatgag
     2341 ttatattttt tacccaagat tcccctacta ttaatacttt tttcatttat atattcctcc
     2401 tacttttata agtttaatat tagacttgca tacataagta tactatctaa atactcctta
     2461 atttcaacat attcatcaat tgaatgacat tgttctattg aacctggccc taatataagt
     2521 gttggtattt cagctaaatt tttcattact ctcgcatcat tgccagctgt tgaacctttt
     2581 atctctaatt tttttcctag agtttcttct agtattttat gtgcattcac tacaaaatca
     2641 gaatccttgt ccatctcaaa acctaaccct gattgatata tgtttatatt tggtttatta
     2701 tctttaagcc ataaatctcc attagccctt gtcattatga catttgttac atcatttaca
     2761 acttgttcat aactcattat atttggcaag aagtgtacac ataaattaaa tacacactta
     2821 tctggcacag tagaacctgc tgttccacca tttattacac ctaaatttat tgtaggtgaa
     2881 ggtaatagtg gatgtttata tatcataagc cagttatgtt ccaacccttt tatatcttga
     2941 agcaacaaca tagccttctc aattgcattt acaccctccc acttactacc acagtgaaga
     3001 gacacacctt taacctcaac ttcaaagaat acaaatccca tatgtgcaac tattaagttt
     3061 tgttctgatg gttcacaaat tatgcaacaa tctccaccaa taccattcat aactgcattt
     3121 atggtaccat ttccgccacc ttcttcatct actactgaca ttatttttac attaccaggt
     3181 acatttagac cactatcttt tataagcttt acagctaata ttgatgctat aagtccagat
     3241 ttcatatcag cagttccaag tccataaagt tttccattat cttctgtagc acgatatgga
     3301 ttatatttcc acttacttat atcacccggt ggcatagtgt ctacatgacc attaaataca
     3361 attgtcttac caggtaaatc atcactatac ttacaaatta aattatatct atcttgataa
     3421 ttgtgaccta gatttccttc tttatatata ttttttgctt ttttaattaa ttcttcgctt
     3481 atttcttgtc tatctacact aaaacctatg taatttgcaa gtttttcaat atattcctgt
     3541 ccttcttttt caaatccacc tagtattcca tgaccaacat cttcagtctt tatacttaca
     3601 agttctttta aataatctat gtattcttgt tttttactat ttaatgtatc taagagaatt
     3661 tttttcatat ttcctcctaa gatttatttt tacatttaaa atttcaagtc taatctcatc
     3721 tatttaagcc atagtaaact ttttcactta gcatattccc attcaatgta taaagatgta
     3781 tgcaaattaa agtataaaaa tatatacaaa aactcaataa tttttatagt ttataagcta
     3841 aaatttaatt actatttaga tttagctgat ttttttaatt ttatattatt tatccatata
     3901 gcaaataaca ttaccaaacc ttttataatc atttgattat atgaagatac acttaataag
     3961 tttaatccat ttcctaatat tcctattatt ccgacaccta tacatgtacc tactatagaa
     4021 ccagaaccac ccatcatatt agtaccacca agcacaacgg ctgctatagc atccaattct
     4081 gctccatctc caacagttgg tgaacctgat gccaaacgtg atgatagtat tatcccagct
     4141 aatgctgtta gtagtccagc tagtgcgtat acttttacta taactttatt tacttttact
     4201 ccacataatc tagcggcctc ttcatttcca cctactgcat gtacatgacg tccaaaatta
     4261 gttcttttta atagaaaatg tcctgctatt acaactataa tcataattat aactggtatt
     4321 ggtattggtc caatatatcc tctaccgagc atcttaaatg catctgtatt tactgatata
     4381 ggtcttcctc ctgtaaatac aaagcttgcc ccacttatta tcgttaacat ggctaaagtt
     4441 acaatgaatg gaggtagttt ttgtgttgaa ataattgcac cttttatgac tcctaatatt
     4501 gtccctattg ctaatgcaag tatcatacct ccccatatag gtgcattata attcactact
     4561 gttgtagccc aaaatgtacc tactaaagca attactgctc ctacacttaa atctattcct
     4621 cctgtaatta ttacaaatgt catacctact gcaagtattg catttattga tatttgtctt
     4681 ccaacattaa ttaaattgtc taaagttaaa aagttagatg aagctacact cattattata
     4741 aaaattatta caagtcctac agctgcactc ccttggtctc taaatacact tgttaatttt
     4801 gtcaataatg gagaagattt ttcttcttta tgcttagtta ttgaactcat taattttacc
     4861 tccgaaagcc aattttaata cattattttc aaccatttct tcaccttcta aaaatccact
     4921 tatatgacct tctctcataa ccattattct gtcactcatt gatattaatt ctggtatttc
     4981 agaagatatc attattatac ttacaccact ctccactaaa tctcccatta gattgtatat
     5041 ctctgcctta gcatttacat ctatacctct ggttggttca tccataatta atatatctgg
     5101 ttgtaataaa agccatcttg ctattatagc tttttgttgg tttccaccac ttaaactttg
     5161 tataacttga ttcatatgtg gtgtttttat ctttaaagct tctatgtatt tttctaaaat
     5221 atctttttcc ttttttctat ctataattga tttaaactta gatatctttt ttaacccagc
     5281 catagttgtg ttttctttga cactcatttg taaaaacaat ccagtacctt ttctatcttc
     5341 tgttattaat cctagtccat tttctattgc atctgatgga gttttaaact ttacttcttc
     5401 attgtttaaa tatattttcc cagaatcata tgttctaagt ccaaatatag cttctactat
     5461 ttcacttctc ttacttccaa ctagtccacc tattcccaat atttcacctt ttcttatatt
     5521 aaagctaata tcattaaaaa cattgttctt agttagactt tccactcgca taatttcttc
     5581 ttttatttct acttttcttt ttgggaaaaa ttggtctagc tttctatcta tcattaaatt
     5641 aactaattct tcctcagacg atacatcttt ggtattcaaa gtcgaaatcg ttttcccatc
     5701 tcgcattata gttattctgt cacaaatctc aaagagttct tccatcctat gggatatata
     5761 tattatacta actccactct cttttaaatc atttataagt ctaaaaagta tttcagtctc
     5821 ttttttactt attgaagaag taggttcatc cattattaat actttagttt ctgatgataa
     5881 aactcttgct atttctacca tttgtttttg agctatacta agctctttga ctaagctatt
     5941 tggatttata tctattccca tattatcgaa aatcttttta gcctcttcat tcattttttt
     6001 gtcattggtt aagactttgc catttatttc ttttcccata aaaatatttt gtgctactgt
     6061 taattcgtca aaaactgtta gttcttgata tatggcattt atacctatct ttttagagat
     6121 atctggggat atattctcta tttctttgcc ttcaaaaata aattttccag aagttttcga
     6181 atgtgcccca gttaaaatct ttatcattgt tgatttacca gcaccatttt cacccagtag
     6241 tgcatgaact tcgccatgaa ataactcaaa attaacatta tctaatgctc tgacaccagg
     6301 aaattcttta gcaatattac ttaacttcag aattatatta ctcataatga cactcctttt
     6361 taaataggat ttctttcaaa taatctcatt ttattttact aattcttaaa gtcttttata
     6421 ttttccttat cataaagtaa agctggtact ggtatattct tttctacctt ttcccctttt
     6481 atagctttta ctactgcttc tacagctaaa cgtcccatct ctttaggttg ttgttgaatc
     6541 atagcaagaa tagttccatc tgttattcca cttgatgctt cttcagttaa gtcaaatcct
     6601 acaatcttaa catccttatt ttttgcagat tcaagtgcag ctttagctcc tagtacacta
     6661 ttttcagctg ttgcatatat tatatctaca tctggattag attgaattaa gttttctact
     6721 gtattaagag attcttctct gtcatatcct ggttgtgaat ttaatatctt aacatttgct
     6781 gaacctttta atgcatcttt aaatccatct attctctgca tttgtatttg agattttaaa
     6841 tctgtcacta ttgctatttc tgactttcca cctaactttt catctatgta ttttttagtg
     6901 tattccccta attttttacc agcatcataa ctaactgttc ctacataact tgtaatattt
     6961 tcagtattta atacagcatc tactgtaact actggcattt tttcaaatgc tccttctact
     7021 gctggtacta tagccgaatt atcagttcca agtactatca ttccatctac attttgttgt
     7081 ataaaatctt ctattgctga tgtttgctta gctacatctt ggtctgggtc atttactata
     7141 actttcacac ctaattcttt agctttttct tttattccct catcaatatc tataaaaaat
     7201 tgatgttgaa gagttattgt agatactcca atagttaaat ctttcttgtc tgaaccacct
     7261 ttattatctg atttacttgg tccttccatt gaacaccctg ttaacattcc tattgctagt
     7321 attccagcaa ttcctaaaga taaaattttt ctcactctca taattaacct ccatttttaa
     7381 tagttttaaa tttgaacaaa ttagattttt atttattatt taaatatttt tcaacatctt
     7441 ctttataggg cataccagat tgtgcaccta gtttagttac agaaagacct gatactacta
     7501 ctgcatattc tactgctgta tataaatttt tcccttctgt tatagccact gccaatgctc
     7561 cattaaatgt atctcctgct cctgtggtat cttgaacatc aacacttata caagatacat
     7621 gcttgatttc ctcaccatca taaaatctag ctccattttt tccttctgtt attacaagtt
     7681 tatttggata tttttttaat acttcttcta tcccttcatt tgtgtcaaaa actatcttat
     7741 attcatgttc atttggtgtt aagtaagtta ctttttctat tatatcttca tttagtttta
     7801 ctgctggtgc tggatttaat aaaaccttaa tcctattttc aaaacagaaa ttcactacat
     7861 aatttattgt ttttaatgga atctctaatt gtagcaatac tatatctgca ttctttatct
     7921 cttcttcatt ctttttaatt aattctatat ctactagctc attcgtccct ggtacaacaa
     7981 caatactatt atcattttca cataatgtta taacagcaac accagaactt ttgtgattat
     8041 ttctggatac taaactataa tcaactttat tcgttctaaa atttcttatt aactcctctc
     8101 ctaagccatc ttcccctatg catgatatca tttttacatt tgctcctagt ttagaagctg
     8161 agatagcttg attagcccct tttcctccag gtgatgtaaa aaaactatta cctaatactg
     8221 tttctccttt ttctggtctt ttatctacag aacataccat gtccatgttt acacttccta
     8281 tgacaactat gtttcccatt acattactcc tttacatatc tatttatgtc tctcgtgctg
     8341 ttattcactt tcttttgttg aatctcttat gactaattct ggtttgaaaa tcaattcatc
     8401 aaacgattct tttttatctc ctcttaaaca atttatcaat atttcactgg ctttatatcc
     8461 tatatcataa gatggctgtt ttactgtggt aagagaaggg cttattagac ttgatgtttg
     8521 aatatcatca aatcccacga cacttacatc atttggtata cttaatcctc tttctttaag
     8581 acctcttatg acacctattg caatcaaatc atttgcacaa aatattgcat caaaatctat
     8641 cttttctaaa ttttttataa attcttgtcc ccattcaatc ttatattttc cttcaaatac
     8701 ataagcagga ttgtagctta aattgcactc gattagagcc tttttatatc cttctaatct
     8761 atctactgct atttcatttt gaagtggacc acttaggtat attattttct tatttccaat
     8821 atctaataaa tgttttgtag ctatataggc cccttcaaag ttatttattt taacaattcc
     8881 ttttaaggtt tctttgttca tacttatctt cttatcaatc aagacagttg gtatttttat
     8941 atctttataa tttgcttctt tccagtttgt atttgaagat gctgcaaata taattccatc
     9001 tatcatcctc tctgatagtg ttcttattcc ttcatattct tttttagcat cttcatctgt
     9061 attacataaa attatattgt agccttcttc attagcttta tcttctacac ctcttgatac
     9121 atctgtaaag aatggatttc gtatatctgg tataagaagc cctatagtat tagttttttt
     9181 agttactaaa ctttgtgcta atttatttgg tacataattt ttttccttca ttattttcaa
     9241 aattttttct cttgttgcct gacttatatt ttcatcttta ttattaatga cttttgacac
     9301 agttgtttta gaaacaccag ccaattcgcc aatttctttt atagttatac tcaagaagtt
     9361 cacccccaaa atttaggaaa ccgttttact taaccggtta cctagatact atcacatctt
     9421 tttttcattt gcaaccattt tatataaatt ttgatatttt tccctttttt caaaatattg
     9481 caacttataa ttcaatttta atatttaaat taatctgcaa aaaaaaatta atatactttt
     9541 ataattatct aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg
     9601 ctctataatc atttttttat cattttcaat cccacttcca acagttgtca aataatcacc
     9661 aacaattgca gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag
     9721 ccttccacca gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt
     9781 ttttagtatt tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg
     9841 atttaaaatg tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt
     9901 tagtctctgt tcttcactct cattcattcc aattataccg cctacacata catctagccc
     9961 agctttttta gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat
    10021 attatgaaaa ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga
    10081 cttcaacatt ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt
    10141 agtattttcc ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat
    10201 acctttacca cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc
    10261 tctatttaag atttcattgt aatctaataa tgaatattct tctacaccag ttttataata
    10321 agaagactga gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat
    10381 cgtacataaa tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa
    10441 tagaatatca aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc
    10501 ataacttatt tctttttctc tcaatacttt attttttaat tttattatgt atttttccat
    10561 atatcctcct atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg
    10621 catatataac atttattggt caactttatt ttatacacaa gtttactata cacaaaaaaa
    10681 tactgcttca aaataatatt agtatatgga ttatacatat tattattaaa gcagtatttt
    10741 ttattagaat ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc
    10801 tttccattgt aataacctct aagccttcat attctctaaa tagccatttt aaatccttca
    10861 tggcttgatt aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct
    10921 catactcttt gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat
    10981 tgtttacatt agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact
    11041 cctttgatac ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt
    11101 ataattttat taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta
    11161 ttctagttcc atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttgc
    11221 aaatcaaatc atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta
    11281 tatcatgggc aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca
    11341 taacaaaagt tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt
    11401 ctgccatctt ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag
    11461 ttggacagac agttggacat tctccacatc cataacatac atcagaatct ataactgctt
    11521 ttttatccac tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta
    11581 cacacttctt ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg
    11641 cacttgcaca tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac
    11701 ctttaaaatg actcattact atcattgcag aagaattata aatttctcca cctattttta
    11761 cactttcaaa atgttttttg tctattttta cattctcata atttctacta tataatccat
    11821 ctgcaattat tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg
    11881 taagatggtc tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa
    11941 atggctttcc ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca
    12001 tatatgtagt atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt
    12061 ctattaaatc tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt
    12121 tattttgcga atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac
    12181 ccctttaaaa tatttactta tactaatttt atcactttta aagtaatttt tagtatattt
    12241 taacataatt aaatattctt atatttattt gatacaaata atattatttg tattattttc
    12301 tacacttaat tctatatatt aaaaatcaaa aagacttgaa acttaagtcc aagtcttttt
    12361 gatttttagg tgtagttttt aacaacataa gattacttta ccttgtgata aaggtattat
    12421 taaactggtt ttttattaaa taaaataaca gctgctagta aacatactac agaaataaga
    12481 actgttataa taattggaat aataaattct ggcattgcca ctttccctga aatcaagtct
    12541 atattttttg acatcaagaa attaggagta taaggtgcaa tctgtttagg aatagaaatt
    12601 aaactcaata aaagagttgc caccaataca agtaaaacac tggcaaatgc tggtctaaat
    12661 agtacacacc caagaattaa tacactaata tacataattc ctataagcca taaattaaat
    12721 gctgaaaata taatatgatt aagatttgca gttggccaaa aatacattgt atatccataa
    12781 gcaaataaaa aactcaacca aaaaccaatt gtcataataa tcaccgtaac agagaattta
    12841 gacaatacta tagatgaacg tgataaacct ttagtcagca taataacaag tgtcccttta
    12901 gaatattcat ttgataaaat attacaaaaa ataattagag ttaaactcat accaagacca
    12961 gagatattgc taaaaaattg tgtccaagaa tctaaagcag ttggtgcagc agtaactttt
    13021 aaagtcggtg caaaatgagc aatcaagtct ggcataaatt ttgcagaaat aggactactt
    13081 ataccaaata ttagaaataa aataattaaa ctaaataatt tataatttct taaattctct
    13141 ttaaattctt tttttgtaaa tgctatgtat tctctcactt tactacctcc aaaaataaat
    13201 tttcaagtga taattctatt agttccatct ttactggaca aagattcatt tctgcgagtg
    13261 tagatataac tgttttttgt attgctttta tatctttacc atgcaaaaca atttccatat
    13321 tagtttcttc tgaatctttt agtaaggatt taataccatc actagactta aacttttgaa
    13381 tttcatcatt acttgcaaac tccagtaaaa gtctatcctt tccgtgcata ctttttattt
    13441 ctgaaagtgt accactaagg gcaatgcttc ctttgtttaa tatagcaaca tggtcacaga
    13501 ttctttcaac atctgatagt atatgtgttg aaaatattac agttgttgag tctttgattt
    13561 taagcataat atctaatatt tcttttcgac caacagggtc aagtgcactt gttggttcat
    13621 cacaaataag taatttgggg cgtgaaagca aagcttgagc tataccaagt cgttgcttca
    13681 ttccacgaga aaacccacct attcgtctct tttcatttct aagacccaca agtgataaca
    13741 actcttcact ccttatctgt atttcttttt ttgaaagtcc cgtaatttca ccacaaagag
    13801 aaagatattc aagtggtctc atataattgt aaaactctgg aacatcaggc agataaccta
    13861 catgacgatt agaacttgtt ttcccatagg tcactttctc accacaaaca tctatagttc
    13921 cactatctgg tttaagtaac ccaagtacca ttttcattgt tgtagtctta cctgcaccat
    13981 tcttacctac aaacccaaag actgaccctt ctggtacagt aaaatttaaa ttatctatta
    14041 tctttcgatt accaaaacct tttgataatc catttattga gacaatattc atcattcttc
    14101 acctcttcct attgtaaaat acaaaattgg tcctattaaa ctaataaaca gtactacaat
    14161 aatccatatt gccttatttc caattttata atttggatgt ctcaacacat gaacaagtgc
    14221 agtaattata agtattaaat ctaaaataat aacaggaacc aaaagtggta agtattccat
    14281 caaattttcc ataatcttat tcctccaata aagataattt ttctataaga aatttgtatt
    14341 ctttgttttc acttaataca gaaaacattg gattattttt aatagcagca acaatacctg
    14401 cttttacgat tgtatcatca cgtgtaatac ctgttccaat ttcagaaatc catctgtcaa
    14461 gtttattaaa aaagtcatcc ccatgtatta taattgggta tatatcacgt gttgctaaat
    14521 ctacatattt tcgtaatgat ttaatagctc cttcattatc accatgtatt aaattaattt
    14581 gtgcctgtgt taaatatgaa gaaagcataa tagcaggaga taatgtatca agctcaaata
    14641 tatctgaaat tgctagaata cgtctttcta tttccttcat ttgtaattta tctgcatttt
    14701 gcaaaatacc cactaggttt tggactaatg atataaggtt ctgatatgca ccaagttgaa
    14761 atgtttctct tgcttcatcc atttggccat tcatcatttg cccctgtgca agaagtatag
    14821 attcatttat catgggaaaa ttgctgtttt gcaataaatc aatcacttgt atgggttgat
    14881 ttaacaatat ataacaagtt gcctccatac tttttgcttg acggcaaata tctatgtcat
    14941 tacaagtttc ctgtatacga ataaatattt ctaatgcttc actaattaat agttcacgct
    15001 ttttttcatc tacaatatca tagtgattaa ctattaaaag acccatccga aaaatcaaag
    15061 gaaaacatga ataatatttt tttatgattt ctcttatttc atccatgact tcatcaaatg
    15121 gtttaaccgt aaagtctttg cacagtttat tatacagttt attaatatct tccttcatca
    15181 tttgaggttc ataacatatt aactcatcaa cggtaatgtt gaaatatgtc gcgagttgcg
    15241 gtaaaaaggt tatgtcagga tagctttgtc cagtttccca tttagataca gaagctttag
    15301 aaacgcctat ataatttgca agttcatctt gtgtaattcc tttttcttta cgctttaaaa
    15361 tcagcgtttt tgcgatgttt aattcattca tgtcaatccc tccttatgta taattatatc
    15421 cttaaacatg taaagctaac aagcgatttg aagttgactt ttgacgaaaa aatcaaccac
    15481 agagaaacaa ataaacaagt atataaaata tatatttaaa tttatcttta atctcttttg
    15541 taaaatatgt aaactttgta aataaaatcc atattatata aaactaatag ttaatctatc
    15601 taaatacagt ttttatttat cacgttttta aatattttgt gtaaaacctc ttttaatata
    15661 aacaccttat tttcatttct catttcatat tctttttaaa atactgaata taactcttcg
    15721 attttccatt taattctttt ataaagttat tattcgtatt tattcctcta attataacag
    15781 gcaactatca tgtattggta tttgtctatt atataaaaat ctataaaaga aaagcagtca
    15841 atcatttaga ttaactgctt gtacatatat cttattaaac tgtataaact ataattttta
    15901 ttattctcca ttttcatccc aagtcataaa tccccatcca ctatctggat aagtatcaat
    15961 agacttttct ctaatcatat aaccaacttt ataacactct tttgttattt tattagatag
    16021 tatacatcct tctccattta tctaattcca tttaattaac ttttcttttc aaccttaact
    16081 tttttaaaat catctttttt aataatttca tacttatttt ctcttcaaca tcatcaaatg
    16141 agggtgtctc aaaattaact ttttacttca taaggcactc ttttttgtat gaagtaaaat
    16201 atattttcat catttcaaca ataagtaaga ggcttatctc tattttgaga cagccccatt
    16261 tgatggtgtt gaacatttaa ataaggttga actcgtttta tctctctttt taagatatct
    16321 gtaaatttgc acataaagtc tcttcttctt ttacaattct aatagtttta ttaaagccaa
    16381 agtatatatc tccatattct ttcgtatatg atttattggt actctttcat ctactccatg
    16441 accaccagtt ttatacgcta agaacttagg tccaaacgcc accatatctt taaaccaact
    16501 tgcatatgtg ccaccaccca tagcaattgt atatccattt tcatctgtta cttcttcata
    16561 ggcttctaat aattttttaa gtactaaatt actctcatcc acataatgta agtctttatg
    16621 agcaataacc tctattttcc aaccattaca aaaaaccttt ttgaatctat ctattatgtt
    16681 atttaaaact atatttttac cataccttat atctaactca catacgattt tttcctcatt
    16741 tacttttaat attcctaaat ttaaggtagt gtttttgaat acctcatcat attgattaat
    16801 ccccaatgca tctccataat aatcactaca aaggtatttt gaaactaatt ctatatcttc
    16861 tctatgctga aaatccatat tatcctcaac tatctttatt accattttag atatagcatt
    16921 ttctcccttt tcaggattac ttgcatgtac agctactcct ttagtagtta actctgaaat
    16981 aattccatta ataatttttg cagtacaatg agatggaaca acattttctt tagttcctcc
    17041 ccttactatt atgtaaggtt tacttttgtc aattttaatt tctttttcta tccttatatg
    17101 aacccttcct ttttctccat taaccactgg atacatagca tctggagtaa atcccatact
    17161 tggaatttct tcacctttat ctaagtaata tttgatacat ttcatacctg tctcctcatt
    17221 tgtaccaaaa attaatctta ttcttttatt aattgtagga tacatatcct ctatgttttt
    17281 caatacatgt ataagcatag atacagcacc cttattatct atgacacctc ttccataaat
    17341 aatattgtca attactgctc ccgaaaatgg gtctacactc cacccctctc ctggtggaac
    17401 tatatctaaa tgcattggaa tccctattaa ttcctctccc tctccaattt ctgcatatcc
    17461 aatataatta tcaatatttt taacactaaa acctaaacta gcacagtaat ctaaagtttt
    17521 atctaaagat tttttaggtc ctataccaaa aggacattgt gaaataggtt caccttgttg
    17581 tgaatcaatt tttatcaatt cttttagaaa ttctatggtt ctatttaaac tattatctac
    17641 acttctgctt atatccataa atcaacctac tttctatctt tttaaagcct gtccttaaag
    17701 aacaggcttt tagcattaaa aattattttt ttgtaaatac taattcacaa acttcatcat
    17761 catgagcaca tttttgagca aaatctaatt taacaccttg tgcgcagtca actcttccat
    17821 aatccccata acaagctaat ctacaaattt cttttctttc ttctttagtt aatccatatt
    17881 ctttccaagc agcatctaat ggacagtgat ggaattttaa aacgccattc tcatctgtat
    17941 tttctactaa ttccatttca aacacttttt gtcctttact gttatataac atttctgcca
    18001 tttttcctgg agtatctgct acgttatatt tttgacctct cattttacca aatccatata
    18061 ttgcttcttt acatatatca tctggattag ctcctaattt ttcagcttct ttcataagaa
    18121 gatagaacca tactgctctt tctcctgttg ctcctctaat tccttctaaa tattcctttt
    18181 ctgtatacat tttttctgac atttctttct cctcctaaaa ttaaattcta tattttaatc
    18241 actgtgaatt agaatattcc aatgaatgac cctaccaatg ctaatacaac taaaattact
    18301 aataacttag ttattgtcca cttcttgtat ttcattcccc aatatacgaa gaatacaaca
    18361 ccaagtggta ataaacctgg agcaatcata tttaatgtat cttgaacaac aattgcttta
    18421 ccaccaatat caaatgatag cggagttgaa agcttaactg tagttgcaga taatgctccc
    18481 atcataaaca ttcctagaat tgaagcacca tcaattatct tattgacaat accacctgat
    18541 agaattttct taattgaatc tctacctaaa gaatatccta aatgaaataa gtaataacct
    18601 tcgcaaaata gtaatatact aaacattatt gggaaaaatg cacctataac acttccttct
    18661 gctccaaatg aagcacctaa agctaagaat attgtttgaa atgtaccaaa atctaaagta
    18721 tctccaattc cagccaaagg tcccataaga ccattcttaa cacctgaaat aacttctcct
    18781 gggatttttc cttcagtagc tttctcttct tccatagcta aaactgtacc gtgaattaat
    18841 cctccccata tagcttgagt attaaaaaat tgtaagtgtc tctttaacgc atctactaaa
    18901 tcttcttttc tcttatataa cttttctaaa gccggagtga aacttgcaca aactgctaat
    18961 gcttgcattc tttcaaaaga gttggataat tctgctgtcc accaccatct tagatatgtt
    19021 tttgttacat ctttctttgt aagtactctt gtcttattaa cttcagttgg accttgtcca
    19081 tttttaactt ctttattttt tatttcttgc attatacttc acctaccctt ttgtcttctt
    19141 tcataaaggt aattaaaaga gctatacaaa caccaaatat tgcagctgca gtaattgata
    19201 tttcaaaata ttgaaccata aaaaatccta taataaacat tggtaaaaat ttatttttac
    19261 ctatagtaaa tattgttgtt gcaaaaccta atgcaggtaa tataccgccc attactgtta
    19321 atccattcat taaccattgt ggtataacat ctaataatga ttgaactaaa tctgctccaa
    19381 aaaagttaca tacaaaaact attggaaacc ttaaaacaaa cccaaatatt aaagaataaa
    19441 gtgttgcaca tctccagatg ccttttgtat taccttcttc agcatactta tctgccttat
    19501 gaaccaatac agcattacca gttcttctta agttattaac taatactcct ataacaccta
    19561 aaggtaccgc aatagatact gcaacttctg cattaacacc agtttgaagt gctactggta
    19621 ttgcaataag agcagctaga catttatctg atggaatatt tccccctgct gcgaccattc
    19681 ctagataaac catttctata cttgcaccaa caatcatacc agttgttaca tcaccccaaa
    19741 gtaatccaag gctaaaacct ataaataaag gagactttag tgttgagaaa agggtatacc
    19801 ctgcaagacc tgctgcaaac caataccata atcctgttac aattgccaac atcaaaacag
    19861 attccattta taattcctcc taatttatat tttttattta aatttcttta acgcattttc
    19921 atatgtcata gatgattcct ctggtataac ttgtaggact atttcaaccc cattttcatt
    19981 aagttcatta agttgttcta catcctcatt tcctaatgaa acagctgtaa acaccatttt
    20041 ctttcctgct tcatttggaa tacctcctaa ttgaatcttt tcatatttaa gtccagcttt
    20101 ataagcctta tttgccatat caacattttt aaatagaata aatatatttt tatctcctaa
    20161 agtgttgttt tggaatgctt gtcccgcatc ttctgctgtt aaaataacta cttctactcc
    20221 tgatggtgct gccattttat atatgtcagc cataaattca tcttgaccta agaagtcatc
    20281 aactattatt attttttgag cttgagcgat ttttacccat tttgttataa cttgcccatg
    20341 aattaatctg tggtctactc ttactaatgt tatttttgcc attatattca cctcataatt
    20401 tatatttttt taaattcttt aattaaatcc acacaaaggt tattattttt tttgacgatt
    20461 ctttctggta atgtatgtaa tttatcttct ttgcttaatt cttcatctac gattaaaatt
    20521 gtctgtaagc taagtccatt tattgcatct attcctcttc ttatagcaac agctattgca
    20581 acacttgatg tagatcctcc atataaatct gatattataa ttttcacatc ttctttatct
    20641 actacttctt caacctcttt cataaataca gaaacatctt tttcagaact tagtgataaa
    20701 acttcatatt gatttgacaa tccaaagttt ttatttgttt cttctactaa gacttttccc
    20761 caagttccat ggcacagtat gattacctta tatttattca ttgctaaata aactctcctt
    20821 tatagcgatt aatattttgg tttaattcta cttcttcttc atcaggtacc atttgaattg
    20881 atacattaat attcttttta ataaattcat ctattaaaag aaactctttt ttattaatgt
    20941 atacattttg agatattttc ttttttccta ttcctgtagg tattcttcca acaataagtt
    21001 ttttgatatt aatcccttct tctatcattc tatttgcagt atctaaattt ttaattaata
    21061 caaaaacgtt atcatcagct tgattattat aaaaatatct ccttgattca cttacagtta
    21121 atattgataa atcaatattc tttggtgctg ccatttttat caaaccttta gcaataacat
    21181 cattggttgt ttcatcatct actactacta tcttttttac acctagatgc ttaacccatt
    21241 tatatataac ttgaccatgt attaatctat catcacatct gcaatatttt actaaagaca
    21301 tttttaatca aactcctttt tctattacat tgagctaaga actttattaa tattgtttat
    21361 accagtttgt cctgcttcaa tacatgcttc tgctaactct tctaaacttt ttccctctct
    21421 attaattaaa gcttcaagta gcattggaag atttactcca gtaagtgcat gaaacttgcc
    21481 acctttttta aggtttaaac ttgcaacatt acttggcgaa cctcctaata ggtcagctaa
    21541 tactaaaact ccgtcacctt catctaattc atttaacttt tctacaaaat ttttatttaa
    21601 gtcttcaata ttatcattcc tatttagttt aattgctgct gttctttctg ctggtcctgc
    21661 aataagttca cagcttttta taatttcttc actaaaactc ccatgagtca taactattat
    21721 tcctatcacc aatttcacct cacttttctt gtcacttttt atataagcaa ttgccgtgcc
    21781 aacttatgtg tatcaataac aaatggctat tttacaggat ttaatcaaaa ctttttactc
    21841 aatttttttt aaaaacttat tatttcgata tgtatcaaaa gattttttca acctgtatca
    21901 aatctgtatc aaaaaaagta aaaaaaagat agccttttgg ctatttttaa attttgtatc
    21961 aacttttgaa tttatctgta tcatattgtg attcaaatat atctaatata tttgcatatt
    22021 ccatatctga aataattata tcgaatgtct cttctactag cttaaaactt tcttttataa
    22081 ctaaatataa ttctttattt ctactaattc tttcttcata tgatttataa actaaagact
    22141 cttttattat tactctttca atcatacatg aacaatgaaa tataaacttt actaaaagtt
    22201 catcttgaat tactaaagaa tagtgttcac taattctatt aattacttct tttaatataa
    22261 aataagcctt ttccccattt aaaaaagtca aagttttact aatattatca attagtaatc
    22321 tatcaaaata tctaccacca ttatcttttt cagcatatgt aacttccata tctccataat
    22381 gtttctttat agattctata atattaaaat ataatttctc taatgtcatt tctggagtta
    22441 gagcctttct agttgcttct attacaatag gtgtacttac catatctatt gcttttactt
    22501 cttttcctgt agcatcacga ataactttag caaatgttaa aatagacccc atatcagtta
    22561 aaattaaaat tccttttcct ttatcaattt tattggctat ttctacagct tgtactaaaa
    22621 tatcctgaac tctatcttct aatcccatat taattgccac tgcatggtta caatccagta
    22681 aagtattggc tacattcacc atgctagatg ctgtagattc accatgcatt atgactaata
    22741 ttgctatact ctcctcattt aatcccacat acgataaata aagaaatgtt gctaaaaaaa
    22801 ttgcttcatc ttctggaaac tctactccat acattaatga catgttagat aatatttctt
    22861 ttgctatgtt atattcatat gctctttcct tcataatttc atctttatta ggatgcttta
    22921 ctatatttcc aatctttatt ctttcaatta aattctgaat atgtaaagct atagctttta
    22981 ttaatttatc taatccttct cttttgctcc atttatcatg cttttgtaaa atatatttta
    23041 cagtagttgc aattgaatca ttaacaatgt tgttataagc tgtttgatta ctactattat
    23101 atataaaagt attcattaaa ttataggaat acttctgtat gtctccatct ataacacttc
    23161 taatttgtga ctctttaata ccttcttgtt gaagtttatt ccaagtttct tttatcattc
    23221 cataaaaatc taaatcatat tttttatttt ctattaataa attttcactt tcaaaatcct
    23281 tttcttctgg taaaaaaata atatcatctt ttccaataaa actgaaattt tgtacaaact
    23341 cctcttttct attatttcca tataatgatt ctctaatatt atttggtaga agagagagcc
    23401 ttacatatac acattccctt ttataggtca tataatctaa aaaagctctt gcacaaatta
    23461 attgtatatc gctttttaat tggcctatat ttcccttaca cttatataaa ataaatgcct
    23521 taataacttc ttttgaaacc tttaacttaa caccaatcct aacactctct tctttaaaaa
    23581 aataacatat aagcttcatt ctctcttgaa agcctctttc ctctaaagac ggaatcttta
    23641 tagttacagg tattcttctt aaaaacgttt ccaacattgc tgtttctggg tcttctgtag
    23701 tggctgcaat tatcataact ctacactcgt gtaccttatt agcctctcct aaccttctat
    23761 atatcccttt atccataagc agaaatagca tttcttgtcc ctctgcagat aatctatgaa
    23821 cttcatctaa aaacaatact ccattattag cttcatgtac aataccatac ttatctttat
    23881 cagctccagt aaatgctcct tttacatatc caaaaagctg cgctaataat aattgctgat
    23941 tatcagaata atcagcacag ttaaatatga taaatggtgc attatcacta tcttctttta
    24001 tagttaaact atacttatac atatattctg caaatataga tttaccaact cctgtaggtc
    24061 ctattattaa tgtactcaac cccttaggtg gatatattac tgctgcttta gcctgttcta
    24121 tttgggacct taaacttcca tcacatccta ctaaattaaa aatattgttc tcttttttta
    24181 tactctctaa aatatactca tctttgatag aatcattttc actatattcg tttatagagt
    24241 taatattatc aaatatccct ttatttagtt tctttcctaa aatactctca atactttctt
    24301 tatataagta ctgaacaggc ttacccttta tctttataat aatactttct tttattaatt
    24361 gatttaattc cttactagca ttacttctat ttatacctac ttcagtagct atatattctg
    24421 catcaaatcc aaatctaatg ccattattaa agttcaagtg ttcttctgtt aaccttttca
    24481 agcaaattaa aactttttct ttccttgata gcttcttttg tgtcatagtt aacctcccct
    24541 ataagcagta atactgttaa acatttatta ttaagcttaa ttctacaata ttttaatatt
    24601 tttgtctaat tatgtaacta ttgattttct ttcctattcg ttttataata taaattacgt
    24661 taccctcatc ttttatagag gaggaattta tatgaatttt ttatcagatt tacacacaca
    24721 ctctatagtc agtggtcatg gctatagcac tttactagaa aatattaatt attgtaaaga
    24781 aaatggaatt aagatactag gtacatctga acatggtcct aagatgcctg gttctcccca
    24841 caaatggtac tttcacaaca ttcaaaatat cccaagaatc ataaataaca ttataatttt
    24901 aagaggctgt gaagcaaata tactagatat taaaggaaac atagacctag agccttttgt
    24961 aattcctcgg ttagattatc ttatcttgtc atttcacgaa gctgtgtttt ctccaaatac
    25021 attagagaat aatactaaag ctttaataaa tgcaataaat aagcatgata atattgaaat
    25081 attaggacac cttggaaacc caaactaccc aatcgactat gaattaatca ttaaattagc
    25141 tatagaaaag aatatcttaa tagaaataaa taattgttct attaaaggcg tgtcaagaaa
    25201 tggtagctcg gataactgta agtatattgc tacactttgc aaaaaatatg gtgctaaaat
    25261 catactgact tcagatgctc acatttgttt tgacataggt aactatgagt attctgaaaa
    25321 tatcttaaag gaaattaatt ttccagatga attaataatg aattatccta aaaaacttat
    25381 taatcatttt caccaaaaag gtaaattact agatgttgat tatacaaata tctaagtaat
    25441 aatacatgct tactttaact cccataaaaa ttttaatact atttatatta tatgaaataa
    25501 agaaattata ataaagaagc tatttaataa agcttaatta cttttatgaa cttgatggat
    25561 tgatatggtc tatgatagca atagaaatta taacattttt aataaactta gttttatata
    25621 ccttatagta taaatctaaa aaagcatcat ctaaaaagga gctgtcacat gagcaaatta
    25681 aatttgttct atgatagctc tttttttact tattttaatt aggtatattt ttaattattt
    25741 tatataaagc ttatgttcta agtaaaatta tgcataacaa acaaaggtat aaaaatacca
    25801 ttttttcaga taatatttaa ttataaataa taaatttaga atacttcttt taaatgatga
    25861 gagtctagaa atttttatgt gtattcttta aataaaaatt tccagactct agcccctttt
    25921 tgaatatttt aaaatttaac ttaaactaag caaattctat aatagcttat ctctaaatat
    25981 ttaaatttaa tttatc
//
LOCUS       DAENCV010000048        25406 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAENCV010000048 DAENCV010000000
VERSION     DAENCV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.048"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            22..588
                     /locus_tag="KQD82_002017"
     CDS             22..588
                     /locus_tag="KQD82_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG4380487.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            893..1354
                     /locus_tag="KQD82_002018"
     CDS             893..1354
                     /locus_tag="KQD82_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380488.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1422..3668
                     /locus_tag="KQD82_002019"
     CDS             1422..3668
                     /locus_tag="KQD82_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380489.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3661..5493
                     /locus_tag="KQD82_002020"
     CDS             3661..5493
                     /locus_tag="KQD82_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380490.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6212..7489
                     /gene="hflX"
                     /locus_tag="KQD82_002021"
     CDS             6212..7489
                     /gene="hflX"
                     /locus_tag="KQD82_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG4380491.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7722..8219
                     /locus_tag="KQD82_002022"
     CDS             7722..8219
                     /locus_tag="KQD82_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380492.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8269..8793
                     /locus_tag="KQD82_002023"
     CDS             8269..8793
                     /locus_tag="KQD82_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG4380493.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9105..10469
                     /locus_tag="KQD82_002024"
     CDS             9105..10469
                     /locus_tag="KQD82_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG4380494.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKIVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEECEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQKHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10608..11774
                     /locus_tag="KQD82_002025"
     CDS             10608..11774
                     /locus_tag="KQD82_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG4380495.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11891..12673
                     /locus_tag="KQD82_002026"
     CDS             11891..12673
                     /locus_tag="KQD82_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG4380496.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12792..13445)
                     /locus_tag="KQD82_002027"
     CDS             complement(12792..13445)
                     /locus_tag="KQD82_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4380497.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13469..14833)
                     /locus_tag="KQD82_002028"
     CDS             complement(13469..14833)
                     /locus_tag="KQD82_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG4380498.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSIFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15491..16921
                     /locus_tag="KQD82_002029"
     CDS             15491..16921
                     /locus_tag="KQD82_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4380499.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            17024..18127
                     /locus_tag="KQD82_002030"
     CDS             17024..18127
                     /locus_tag="KQD82_002030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG4380500.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            18142..18378
                     /locus_tag="KQD82_002031"
     CDS             18142..18378
                     /locus_tag="KQD82_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4380501.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            18395..19444
                     /gene="vorB"
                     /locus_tag="KQD82_002032"
     CDS             18395..19444
                     /gene="vorB"
                     /locus_tag="KQD82_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG4380502.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            19446..20186
                     /locus_tag="KQD82_002033"
     CDS             19446..20186
                     /locus_tag="KQD82_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG4380503.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            20189..20728
                     /locus_tag="KQD82_002034"
     CDS             20189..20728
                     /locus_tag="KQD82_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG4380504.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            20852..22951
                     /locus_tag="KQD82_002035"
     CDS             20852..22951
                     /locus_tag="KQD82_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG4380505.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAVVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(23218..23733)
                     /locus_tag="KQD82_002036"
     CDS             complement(23218..23733)
                     /locus_tag="KQD82_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG4380506.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLASVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(23825..25036)
                     /locus_tag="KQD82_002037"
     CDS             complement(23825..25036)
                     /locus_tag="KQD82_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG4380507.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         9773 a         2905 c         4425 g         8303 t
ORIGIN      
        1 ttaaagaggg tgatattaca tatgagaaaa cttgaagaga tattagaata taataaatca
       61 tttgtagaga acaaagaata tgaacaatat gtgacatcaa aacatccaaa taaaaaaata
      121 gtagtcttat cttgtatgga tacaagatta actgagcttt tgcctaaggc tatgaatttg
      181 aaaaatggag atgtgaaact aataaaaaat gcaggagcta ctataatgca tccttttgga
      241 agtattatga gaagtgtttt agttgcaatt tacgaatttg atgtagatga ggttatggtt
      301 gtagggcatc atggatgtgg tatgtgtaat gttgatacgg ataagttact tggtaaaatt
      361 ttagatagag gaatatctaa tgatgtaata cttacacttc gtagtgctgg aattgataca
      421 aaacaatggt tacatggatt cgattctgca gaagaatctg taaaagatag tattgattta
      481 ataaaagggc atcctttaat tccagatggt attattgtac atggtctaat tatatcacct
      541 gaaactggga agttagatgt tgttgtaaat ggatatgaag ataagtagag tatcataatg
      601 ttatataatc aagatactgt tgaatatgat gttgtttcaa aatttatttt gggacaactt
      661 ttttatttaa aaatatatga aaaggtttat tttaagcttt acaataaaaa atataagtag
      721 ctgactaatt aaaaatctta ctattattta ttaaaattat aactatatga tataaaaata
      781 tttaataaat gaaatttata tgaatttttt ataaatatat tgaaaaaatg catgcagtat
      841 agtaaaataa tacttagctg actaagtaat ataaaaaata aggagggcaa gtatgaatag
      901 caattctgaa gaaaaattaa atgatgtttt cttgcaatta atgcgaatat attttataag
      961 aactcatttt tattttgata aattaggctt atatcatggt caaccccaaa tacttttttc
     1021 tttaaagatg aaagatgggc aaagtcaaaa agaattagca gataaaagga aagtaaaagc
     1081 atctacaact acagttatga taaagaggat ggaaaaagct ggacttgtgg aaagaaaaca
     1141 agatgaaaag gaccaaagag tatctaggat ttttttgaca gacaaaggtt tagaaatatg
     1201 taataaagtt gaagaattta acaaggaatt ggaaagagag ttttttttag gatttagtga
     1261 agaagaaaaa atagtactta gaagacttct aatgcaagta cgagataatt taaaagcagg
     1321 atgtcataag catgaagatg aaaaatattt ttaaagttta aaataatgtt tttcaattta
     1381 atgttattag actaattaat attacagaag ggaagaaatg tatgtttaaa ttaattaaat
     1441 atttaaagaa atcggcatta tcaatagtaa taatagtctg tctattggta attcaagcgg
     1501 tatgtgacct ttctttacca gaatacacat ccaatatagt aaatgtaggt atacaacaag
     1561 gtggagtaga aaactctgtt ccatctgtga ttagagagag tgaattaaat aaaattactt
     1621 tatttatgga taaaagtagt aaggataaag tactagataa ttatactttg ctgaataaaa
     1681 aggattatgt taagtataaa gataagtacc caggtctaaa agatgaatct ctttatgagt
     1741 taaatactaa agacaaagac actatagatg atttaaatgt tatttttggt aaagctattt
     1801 taatagtatc agggcttgag ggcgatagta aagaggtaaa agctatgaaa gctcagttaa
     1861 tgagtaaact tccaccacaa gctactcaaa gtggagatgt tgatatattt aaattgttat
     1921 cagcaatgcc aaaggaacag ttagatacat tgactaaaga gatgagcaaa ggctttgaaa
     1981 gtatgccaga gagtatgata acacaaagct ctgtttcata tgtaaggtca gaatatgaga
     2041 aaataggtat tgatacagaa aaaactcaaa ataattatat attatttact ggtgctaaaa
     2101 tgcttggtat agcaatgatt agtatggtag ctacaataac agtaggattt ttagcagcaa
     2161 gagtagccgc aagtgtaggt agaaatttaa gaagtggagt atttagaaaa gttatgtcat
     2221 tttcaaatac tgaaatgaat gaattttcta cagcttcatt aattactcgt agtacaaatg
     2281 atattcaaca gatacaaatg ttgatggtta tgttgcttag aatagtattc tatgcaccta
     2341 taatggctat aggtggagtg ataaaggttt taaacacaaa tacttcaatg gcctggatta
     2401 tagcagtagc agtagttgct atcttatcat taattgtagt gttattctca gtggttatgc
     2461 ctaagtttaa actggttcaa aagcttgttg ataagttaaa cttagtaagc cgtgaaataa
     2521 ttactggtat tccagttatc agagctttta gtactgaaaa acatgaagaa gaaagatttg
     2581 ataaagcaaa taaaaattta actaaaacaa atctttttgt aaatagagtt atgacatgta
     2641 tgatgcctgc tatgatgctt ataatgaacc ttatcacagt tcttatagtt tggagaggtg
     2701 catacagtgt tgattctgga gcaatgcaag ttggagatat gatggcattt atacaatata
     2761 caatgcaaat aataatgtca ttcttaatga tatcaatggt atctattatg cttccaagag
     2821 catccgtttc agctacacgt atagatgaag tattagtgtc agatataagc ataaaagacc
     2881 cagaaaaatc agaggcacaa gagtttaagg aagataaaaa gggtcttgtt gaatttaaaa
     2941 atgtttcgtt catgtatcca gacgcagaag aacctatatt aacaaatata tcttttacag
     3001 caaagccagg agaaacaact gcctttatag gaagtactgg tagtggtaag tcaactctta
     3061 taaatttaat tcctcgtttc tttgatgtta ctgaaggaga aatattactt ggtggagtta
     3121 atataaaaaa tgtttctcaa catgatttaa gggaaaaaat tggatatgta cctcaaaagg
     3181 gagtattgtt ttctggtact atagaatcca acttaaaata tggtagaaaa gacgcaacag
     3241 atgaagaaat gagaacagca gctgaaatag ctcaagctac agaatttata gattctaaac
     3301 cggaagcttt taaaactgaa atttctcaag gtggaacaaa tgtatctgga ggtcaaaaac
     3361 aaagactttc tattgcaaga gcaatagcaa aaaatccaga agtttatata tttgatgata
     3421 gtttttcagc acttgactta aaaactgatg cagctcttcg taaggcttta aaatcaaaaa
     3481 ctgctgaaag tacagttcta atagttgcac aaagaattag tactatctta aatgcagaac
     3541 agataattgt tttaaatgag ggagaaatag ttggaatagg aacacataaa gagcttctaa
     3601 aaaattgcga agtttatgag caaatagctt tatcacaact ttcaaaggag gagctagcta
     3661 atgagtagaa tgggaagagg tcctatggga aaatctatgg gagcaggaca aaaagcaaat
     3721 gactttaaag ggactatgcg aaaactaatt gcatatttat ctaaatttaa aatatcaata
     3781 attctagtaa ttgtatttgc tattggtagt gcgtcgtttt ctatagtagg tccaaaaatt
     3841 ttaggaaagg caactacaaa gatatttgaa gggctagtga gtaaagtttc tggaggaaac
     3901 gtaggtatag attttaatgc aattggaaaa atcttaacct ttttactttt cctttatttg
     3961 ataagtgcac tgttctcttt tatacaagga tttataatga gtggaatttc tcaaaaagta
     4021 tcctataatt taagaaaaga gatttcagca aaattggata gattgccaat gaagtatttt
     4081 gatactaaga cacatggtga aatattatcg agaattacga atgatataga tactttaaat
     4141 caaagtttaa atcaaagtat gacacagctt ataacatcag taacaactat gataggtgtt
     4201 ttgataatga tgttatctat aagtggaatt atgacattag tggcagtttt aatattacca
     4261 atatcaatgt ttgtaatttc aagaataata aagaaatcac aaaaatactt tagatatcaa
     4321 caagaatatt taggaaatgt aaatggtcaa gttgaagaaa cttacagtgg ccaaacaata
     4381 gtaaaagcat ttaatcgtga agatgaagta attgaggagt ttgataaatt aaatgatagc
     4441 ctgtataatt cagcgtggaa atctcaattt ttatcaggta taatgcaacc acttatgatg
     4501 tttattggaa acttgggata tgtaatggta tcaatcttag gaggttggct tgcaattaag
     4561 aaaacaattg aggttggtga catacagtca tttatacaat atgtaagaaa ctttacacag
     4621 ccaatgactc agattgcaca ggttgccaat ttattgcaat caacagcagc agcttctgaa
     4681 agagtatttg agtttttaga agaggaagaa gaagtccaaa ttgttgaaaa tgcagtttca
     4741 atagatggtt tagaaggtaa aattgatttt gaaaatgtca actttggata taacccaaac
     4801 aagactataa taaatgattt ctcagtaaat gttaaacctg gtcaaaaagt tgctattgtt
     4861 ggacctacag gagcaggtaa aactacaata gttaaactgt taatgagatt ttatgatgtt
     4921 aatagcggtt caatactgat tgatggtcat aatattaaag attttaatcg tagtgagctt
     4981 agagaaatgt ttggtatggt tcttcaagat acatggttat ttagtggttc aattatggaa
     5041 aatataagat atggtaagtt gaatgctaca gatgaagaag ttatagaggc tgctaaatct
     5101 gcacatgttc atagatttat taagacatta ccagatggat ataagatgaa attaaatgaa
     5161 gaagcaagta atgtttctca aggtcaaaaa caattattga ctatagctcg tgctatttta
     5221 gctgatccta aaattttaat attagatgaa gctacaagtt ctgtcgatac tagaacagaa
     5281 gtattgattc aaaaagctat ggataatctt atggaaggta gaacaagttt tgttatagcc
     5341 cacagattat caactatacg tgatgctgat atgattcttg taatgaatga aggagatata
     5401 gttgaacaag gaaatcatga ggaacttcta aagaaaggcg gattctatgc caatctttac
     5461 aattctcaat ttgaagaaga tgaagcaatg taacaaaaaa ctatcttagc atagacatgt
     5521 aattaataag ataaaataaa tgagggtgcc ttaaaatgaa ctttttaatt cataaggcac
     5581 tcttttgtgt atgaaataaa atgtattttt atcattttaa caatgaaaaa gaggattctc
     5641 tatattttga gatagccaca taattttatg tctttacaaa gtgtttattt tattataata
     5701 tgtagtaagg aaaatgttgg aggtgattat caaaatgtat atttaatttt attctttaaa
     5761 ttttattata aatagaattt aattttaata gttgatgatt atattgaagt atatgttttt
     5821 attactttta ttaatcatgc aaataactac ttaaatttaa gaatagggtt aaatataaaa
     5881 tgataattac atataagtta attaaactaa tgtattttta atatatattt agaattttaa
     5941 tccttcttat gatataagta gcttgttttg tattacttag atttgtaagg agttttttta
     6001 ttgaaaaaac tgcataatat atttgggagg aggtgtaaga aagtgatttt ttatataaga
     6061 ttgagaaaat aatgcacgat tatggttatt ataatagtaa ataagaagtg tcatttaagg
     6121 ataagataaa aagcaaaatt cttgatgaat ttaattatag agccataatc tcgtgctata
     6181 gattaaataa aaaatagcag ataaggagaa tatggatatg gcgaaaaaag gaataacagt
     6241 aggattaaac ataaataata aatctgagga ctttaatgaa ctaatgattg aacttgaaaa
     6301 cttatgttca gcatgtgata ttgatgttgt agggagtata actcaaaatg caaagcaggt
     6361 taatagagca ttttacattg ggacaggtaa agttgaggaa atattaaact taataaagaa
     6421 agaaaatata gagatagtta tattttacaa tgaactttct acatcacaac taaaaaatct
     6481 tgaagaaaag cttaattgtg aaattataga tagaacggca ttgatacttg atatatttgc
     6541 acaaagggca aaaactagag aagctaaact tcaagtagaa gtagcaagtt taaaatatat
     6601 gttaccaaga cttataggtt caaatgaaaa tttaggacgt caaagtggtg gagttggtac
     6661 taaaaataga ggttctggtg agaaaaaatt agaacttgac cgtagaagaa tagaagaaaa
     6721 gataacatct ttaaataggg agttggatga tttaaaattc caaagagaaa ctcaaagaag
     6781 tatgagaaga aaatcaaatc ttccaaatgt agctctagtc gggtatacaa atgctggtaa
     6841 atcttcaata atgaataaac tagtagatat atttaaaaac tctgaagaaa agaaagtgtt
     6901 tgagaaaaac atgttatttg ctacacttga aacttctgtc agaaatatag ttcttgcaaa
     6961 taacaaagaa tttttattat ctgatactgt tggttttgta agtaatctac cacatgactt
     7021 agtaaaagca tttaggtcaa cattagaaga agcatgtgag gcagacgtac tgttacatgt
     7081 tattgatatt tcaaatccaa gctataaaag tcatattaaa gtgacagaag atactttaaa
     7141 acaaataggt gcagatggta tacctatgat acatgtgtat aataaaattg atttaataga
     7201 tgtagaagta ttggatagaa ttttagatag tatcgataaa gaaggtatat ttgtttcagt
     7261 taaaaaagat ataaatatag ataaaatgat aaaatgtatt tgtgatagca tatttaaaga
     7321 ttatgtaaga tgtaagttct taattccata tgataaaggt catgtagttt cttattttaa
     7381 cgaaaatacg agcattataa atacagaata cagagaagat ggagctattt tagatgtaga
     7441 atgtagcaat atagaatata ataagtataa aaagtatgct ttagagtaaa ttttgaaaaa
     7501 cttattatag cctaaaatat atttaattta taatttatta taaattgatt gtaaatatga
     7561 ttttatgaaa cggtatagcc ataatgctat gccgttttgc catttataat aaaaataaat
     7621 ttattgacac aatagtaact aattactata atataaagta actaattact atttaaaatt
     7681 atcaaaaaat taaaatctaa gataaagaga agtgtataat aatggatgca gaaagtttaa
     7741 tgaaaaaata tatggtagat gatgatagta gaaaacaagc tatatttgca agtatatttg
     7801 taatgcaaaa taaacttcaa actacatgtg ataaattaga cccagaatta acaatgaaac
     7861 aatggttact tttagctatt gcggactctg tagatgaaac tcttacatta accaaacttg
     7921 gagaattaat ggggtgttca agacaaaatg taaaaaaact agctattgct ttagaaaaga
     7981 aaggttttat agaaattgag caaaatgaaa aagattcaag agctatctgt cttgtgacaa
     8041 aagatagagt ggaagagtat tctaaaaaag taagaggtat gcaggaaaaa gtattacagc
     8101 ttttatttga agattttaca gagaaagaag tggaacagtt attttttgga attatgaaat
     8161 taagtatagg aattgaaaat gtgaaaaatt atgtagaaaa taatgacgtt ggagaataag
     8221 caagtatcaa atcaactttg tatgacatca gagagggggg taatttatat gaaaaatgta
     8281 gtagtgattt ataagagtaa gtatggttca acaaaaaaat atgctaaatg gctttcagaa
     8341 ttattgtctt gtgatatttt tgaaactaag aatattaata tagaaaaggt tatgaagtat
     8401 gatacgatta ttttaggagg aggtctttat gcttgtaata tagcgggaat ttctttctta
     8461 aaaaaaaatt ataaagaatt gcagaaaaaa aatttagtag tatttggcgt aggagctgct
     8521 ccatatgatg atgaagtaat aaagaaatta agagaagtaa attttaagga tgaattaaac
     8581 aaaattcctt gcttttattg tcgtggggca tgggatgaga caaaaatgtc ttttatagat
     8641 tatactcttt gtagtttttt taaaaaatct atagctaaaa aagaacgtca aggtcttaaa
     8701 ccagcagaag cacctttatt gtctggagaa gcatttgatt ggtgtaataa agaaaattta
     8761 actccaatac ttgattatat aagagaactt taagaggtat atatttataa ttatttatta
     8821 tctaaagtta gatttcatag aaacttacta gacattattt ataaaataat attattaaag
     8881 atggataatt taattttttc aataaggtta taattttaaa aaatgtttat tttaaaataa
     8941 attattttat aagcttactt atacttaata aggggctttt tttattggaa gatttatgtt
     9001 atattgagat gaatttatta tgatataaaa aataatgtta ggataaattt aaatcttttt
     9061 ctaaaaatgt attaatataa ttcttaaaga aaggattttt aacaatggat ataaaagagt
     9121 tggttaaaat gcaaagaaaa tattataata caggtaaaac aagagatata tcgtttcgta
     9181 tagaacagtt gaagaaactt aagttagttg tatcacaaaa tgaagaaaaa atacttttag
     9241 cactaaagaa agatttaaat aaatcggatt ttgaaggttt tatgacagag gtaggcatgt
     9301 tttatagtga attaaacttt gctataaaga atataagaaa atggagcaaa ataaaaagag
     9361 ttaaatcaag tatggttaat tttccatcta tatccaaaat tgttccacaa ccttatggtg
     9421 ttactctcat aatgtcacct tggaattatc catttcaatt agcacttata ccattagttt
     9481 ggtcattagc agcagggaat tgtgttatat taaaaccttc tgaatattct gtgtcaacat
     9541 ctagtgtagt taaagacata gttgaagata ctttttcaaa agaatatgtt gcagttgttc
     9601 aaggaagtca agaagaatgt gaaaagttac tattggaaag atttgattat atatttttta
     9661 caggaagtac taatgttggt aaaataatca tgaaatcagc atcagagcat ttaactccta
     9721 ttactttaga acttggagga aaaagtcctt gtatcatttt aaaagatgct aatatagacc
     9781 ttactgccaa aagacttact tggggaaaac taataaatgc aggacaaacc tgcgtggctc
     9841 cagattatgt tttagtgcat gaagatagaa aaaatgagtt aatagagaaa ataaaatatt
     9901 atactaataa gtattttggg gataatgcat gtaataatga gcaatttcct aaaatcataa
     9961 atcagaaaca ttttaatcgg atattatctc ttattgataa agataagata gtctatggtg
    10021 gaaattataa taaggagaca ttaaagatag agccaactat agtagacaat gttaattggg
    10081 atgataatat aatgaaggaa gaaatatttg ggcctatatt tcccatttta acatataaag
    10141 atttagatga agtaatacaa aaaattatac aaatgccaaa tccattggca ctttatattt
    10201 ttacaaaaaa taagtaccta gaaaataaac ttttagaaat gattccagca ggaggatgtt
    10261 gtatcaatga tacagttact catatagcta cgaattactt accttttggt ggtattggtg
    10321 aaagtggtat gggctcatat catggaaaag ctggattcga tacatttact cattataaga
    10381 gtgtgttaaa aaaattaaat ttggatgtcc ctattagata tgctccatac gataataaat
    10441 taattaaggt tcttaaaaag ataatgtagg ttatattgta gaataataaa tataaataaa
    10501 attgagtatt tgtattctga atatatttta tttttaattt ggtatatttg ttataaaagt
    10561 ggtgattttg tgataaaatt taattatata gaaataagga ggaaaaaatg aaaattaatt
    10621 ggaatacaaa atacacaaca atagcaattt atacatttat aatagctgct agtagtatta
    10681 tattttatct agtatcatca caaattgatg tattttctaa taatttagat gctatattta
    10741 caacattaca accttttata ataggatttg ctattgcata tcttcttaat tttattttaa
    10801 agttttatga agacagaatt tttataaaaa gtgagaaatt aaaaaaacta aaacaaagtt
    10861 caaaaagagg cttgggtctt ttattaacat atgcaacagc tacattgata ctttacttat
    10921 ttatgcattt cgtattgcca caagtaattg agagtattgt tggactagca aatgatatac
    10981 ctatgtatgt aaacaatgca actaagctta ttgataaatt aatgactgat ttaaatcttg
    11041 atgagcaata ctttaatttg gcagttgata agtggaatga gtttgttaca tatattataa
    11101 agtttgtaac agatttaatc ccaatattag gaagtatgtt aaagaatgta gcctctagta
    11161 tatggaatat tgtactagga ttaattgttt cagtatactt attaattgat aaagaaaaat
    11221 tctatggatt aagcaaaaaa ataacttatg cagtatttac agaaaaacaa gctgccagaa
    11281 ttttagaatt aactcataga agtaactata catttggaag atttttaggt ggcaaaatat
    11341 tagattcatt cattataggt attctaacat ttgtaatact tacattagtt aaaatgccat
    11401 atacattatt aatatcagtt ataattggta ttacaaatat tatccctttc tttggacctt
    11461 tatttggagc tataccatct acgctaatag tgatgtttgt atctcctata aaagctttct
    11521 ggttattgtt aattatatta ataatacaac agatagatgg aaatattata ggacctaaaa
    11581 tacttggaga ttcaattggt atatctgcat tttggatttt gttctcctta ttggttgcag
    11641 gaaaattatt aggttttatt ggaatggtta taggagttcc aatgtttgca gtaatttatt
    11701 ctataattaa ggatattgtt gaaagtaaat tggacaaaaa gggattaccg acagatactt
    11761 ccgattatat gtagctaaaa aatattatgt ataattaaaa atatataaat taagcaatat
    11821 ttaaagcact gagaaagtta aactcttggt gctttttaga tataaaatta ttatagaaga
    11881 ggtgtttttt gtggcattta tagaatgtaa atttaaatct aaatatactg gaggagaaac
    11941 tgatataatt gtaattttgc ctgtagcaaa tggaaaagaa ctttttagtg gtaaagagat
    12001 atatgatttt gaaggtaaat ttaaaacatt atacttattt catggtttag aagatgacca
    12061 aagctcatgg attagatata ctaatgtaga gcattatgct aaaaataaaa atatagccct
    12121 agtaatacca agagtagaaa ctagttttta tactgatatg gcgtatggac accaatattt
    12181 tacttttgta agcgaggaat tacctaaatt tgttagagct atatttcctt tatcagataa
    12241 aagggaagat aactttgtag ctggtatgtc tatgggggga tatggagctt taaaattagc
    12301 gctttcaaaa ccaaaagagt tttcggcagt tgcaagtttt tctggttcgc ctgatatgat
    12361 tcatgagtta gaaaatcaac aaatagaaac caatgcagac attctatttc acgattttgg
    12421 tagaccagaa gacgctagaa atagtgagaa tgatttactt tacatactta agaaccttaa
    12481 agaaagaaat gaagatattc ctaaaattta tcaattttgt ggtgatagag attttttata
    12541 taaaaataat cagatgttta aaaaatatgc agattctctg ggtgtagatg ttgaatatga
    12601 agagtgtgta ggggggcatg aatggcgtct ttggaattta tggataaaga aatttataga
    12661 tataattgga tagtacactt atggtattaa caggtatact ataaaatatc tttaaatatg
    12721 ataaagactg aaatcctaaa atcaaattat gattttaaaa tttcagtctc taagaattta
    12781 ataaaatttt attaagttct tacacgagtt attattggag taaaattgtc aactatatct
    12841 atatagtatt tactcaattc ctctaaaatc tcttcgcata aatccgtact atgaccaaga
    12901 ttaataagaa actgttttgt aaaatgcttt attacagctt gagaagatat atcgtacttg
    12961 tgtgaagtat aaactctaat tatttcaggt ttcatagcaa gtataccaat acaagaaagt
    13021 attaattcat catgttctat agattcttta tcattaacat atcttttcat gaccatttcg
    13081 gttacacttc tttgatagct taaaatagct tcttcataag ctatatccaa ataaaaattt
    13141 ctatatgctt cgttttttag aaggaaacaa aggtcagcat tcatagcaag aaagaacatc
    13201 atcatagtac cttcttcatg gtagttatag attaaagact gcatttcatt tcttatatct
    13261 tcatatatta tagctcctat ttcgctttta cttgagaagt aatagcttat tagacctaaa
    13321 tttatattag ctctttctgc aatttgccta gttgttgtct tatgataacc ttgctcaaaa
    13381 aataattctc tagcagcact tataatacta gcttttgttt ctttgccctt ttctgatttt
    13441 gacatattta ctgtgtatac ctctcttttc aatctttttt tgaaaatttg ctaactataa
    13501 atattgtaat agaaccacct attataggaa ccaaaattct gtcaaataca gtaagaatac
    13561 caatcatatt taataatgcc aatataacac ttactataac agcatagata gctccttttt
    13621 ttgtagcacc tttccagcaa agaccaatta caaatggtgc aaatattaca cctgtacata
    13681 atggagctgg ccaaaacaat aattctaata aactatcaaa tttaagtgct atgattgcac
    13741 ctattatagc tgtcaaagct gatgcaattt gagaatactt tagagtattt tttaaatcat
    13801 taaattctat attaggatgt aataatttgt tataaatatc tcttgatata atagcactca
    13861 ttgaggtcat taatgaatcg gcagtagaca ttatggcagc caatatagca gctaagaata
    13921 atacagatat taaaggtggt aaagtagtcg atatcatttg aggtaaaacc tgaacagatg
    13981 atatatttgg aaataaacta ccaccaatag ctccaattat tcctataggg aaagcaagaa
    14041 ataatagtat aaatcctgca attacactac ttttaactgc atttttagca ttatcagcac
    14101 attgatatct taaaaaactt tcataagaaa atattgagcc taaaactaat ggtaaaactg
    14161 tatacataaa atcactaatc gatttatttc ctggaaagaa tggttctttt accattgatt
    14221 gtaacataag taaagggttg tcgccaacta attttaagct tatataagct actattggca
    14281 aaaacactat acaaatagca agttgaataa atgctgatac tgcaactccc cacattcctg
    14341 atgtataagt atatataatg ataactataa ctcctataat tacagctata tcataaggaa
    14401 taccaaatgc agtagataca gaaccaaaag aaattaattg actacccata atagctagta
    14461 tagatattgg tgttacaaga ctttgataag ctcttaaaaa tatactatga ccatatctag
    14521 tttcaatata atcagcaggt gttatatcac aagataagct tcttaatttt cctgcgaaaa
    14581 atgcatttat aaagcaagct aaagctgcac ctatagtaga atacattcca acccaaagtc
    14641 ctctttcagc tccccattga actccaccaa ctaagcaccc tcctccaaaa gatgtagcag
    14701 caatggttgc agctagtata ggcaaaggca gatttctacc tgcgattagg aaatcggatg
    14761 ctgttttaac tttttttgaa aaataaaaac caatccatat gtatattgca cacgtaacaa
    14821 ttaaaagtaa catagtcttt tctcctttat aaaatatttt aataaaatat acaaaatata
    14881 tttaaaatat attttatata aaagtagtac gatatttgtt ttattggttt tagattaata
    14941 tagctataat aatttatgat attagatgga aattacgtaa aaatagaaaa ttgaaaattc
    15001 tgaaattttt gcatattgat atttatctca tagattcttt tttgtaattg taacacgctt
    15061 ttatttttaa atcaatagta catgtataaa aataaaagga atttaaataa gaaaaagata
    15121 tttttaatat ctggatgtag tatagaaata tcttgaataa attattcttg aataaaatta
    15181 taaaaaatta aaatcactag aaatatgata aaataattta aaatacgcat ttcaatgaca
    15241 tttcaaaaca agaaaataat aaaaaacaaa cctaaattaa gtctaaatag ctttttagtt
    15301 aattttagta gtaaaaaata aaacttaaaa agtgtttgac aattatgaat tttgagaata
    15361 taattaaatt aaaattatac acatataaaa atatactcct gaatcaagaa tgagatattt
    15421 aagattcaaa atatatatta ttatacgtga ataataaaaa gtaagaaaat tttataaggg
    15481 aggggtagat gtgagtagtc aagaaagcaa accaaaagga aatcttatat caaattttag
    15541 agatgatatg agagacccta agaaaagagc aatatacaca tattcaagtt ctataaactt
    15601 agcgtacaat tttataggtt actttgtatc aactgttaca aatatttttt taactgatgt
    15661 ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt atacaatctt tcataaaaat
    15721 tgctgtagct cctataacag gtacagtatt tgacaaacaa ccattcaaaa aaggaaagta
    15781 ttatccatgg cttagatatg caccagcagc gttagcatta acatatggaa tatttttcat
    15841 agttccatta atgaaaataa gtccagcatt acttttacct ttgatgacag ctttattatt
    15901 aatagcatct tgtgctcagc aagtagtttt cactataatt tatgctattt accctgtagt
    15961 tgctaagaca cctagagacc gagttatagg ctcaactact acgaatatat ttaaagaagt
    16021 tggtaaattc ttagtaggtt tttcatatcc gttattatta gttttatttg cagatgtatt
    16081 aggtggagaa ggaatgggtt atttaggtac ctacttattc tttgcagcag gatgcctttt
    16141 agtattttgg ttttcttcaa cagagataat aaaatctgga gcagaagaag aagttcttca
    16201 aaaagaagag aagccaaaga ttaaagcaag tgaaatggca aaagctttat tcactaatgt
    16261 accacttatg atggcatttt gtttagaatt tcttatatgt ataagatcta taggattagg
    16321 acctttggct ccatattatt ttaaatatgt aatagaagat gaacgtggat tagcagtatt
    16381 tttatcaatc atgcctttgg taagtgtagc attcatgttt ttcgcaccag tgtttataaa
    16441 aatctgtaga gagcaaaaac tagcttcaat aatttctttt tctatatgtg cactatgcca
    16501 cttattggtt gcatggactc cttgggggaa aactacttta ggagtaacta tgttgttggc
    16561 aattggtgga ggtttttcaa atgtagtatc aataataaat cttaatttct ttgccggttc
    16621 ttgtgattat gggcattgga aatcaggaaa agacttacct ggcttaagta tgtcactata
    16681 tccagttgca atacaagtag gagtattact tgcaactact ataagaacag ttttaatgaa
    16741 ctcaatggga tatcaagctg atatggtagt cacagaagca gttaaatcag gttttataaa
    16801 tatgatttct tattctatgg caataccttt aataatagct gttgttatag caatactata
    16861 tccagtaagt gacaagaaat taaaccaaat aagagaagag ttaaatcaaa gaaatgctta
    16921 gttaaaagaa agttaagtag atggttaatt taagctatta aagtaagtag acgcaatagc
    16981 gtttatataa atgaaaatac atatacagag ggggctttta aaaatgatag aaaaattatt
    17041 gagaaatacc gtaaaagaat tacatcaata tgttccaggt gaaccaatag aaaaagttaa
    17101 ggaaaaatat ggtgtaaaag aaataataaa attagcttca aatgaaaatc ctctaggacc
    17161 atcaccaaaa gcaatagaag ctatgattga aatgttaaaa caaggtcaac tttatccaga
    17221 gccagaagct aacgaactta gaagaaaatt agctgaaaag ttagatttaa aaccagaaaa
    17281 ttttattgtt gctaatggtg cagataacgt tatcactcta ataggtgagg cttttataaa
    17341 tagaggagat gaagttatat attgtaaccc aacatttcca tcatatagaa ctgcaactat
    17401 aaaaaatgaa gggataccag tagaagtacc tttaacagaa gattataagt atgatttaca
    17461 aggtatatta gataagatta cagataaaac aaaattaata tgtgtatgta accctaataa
    17521 tccaacagga acaattgtgg atgataaaga gttagaagaa tttttaaaga aagtccctga
    17581 aaatattata actatattgg atgaagcata tatagaattt ttaacagttc ctaattatgt
    17641 ggatggactt aagtatgtaa gggaaaatta taacgttata gtaacaagaa ctttctctaa
    17701 aatatatggt cttgcagggc ttagagtagg atatggtatt gctaaagatg agataataag
    17761 aactttattt acagtaaaag agcctttctc tgcaaatcgt gttgctataa gtggagcaac
    17821 agcagcactt gatgatgaag agtttataaa agaaacatat gaattaaata gagttggaat
    17881 ggcttacttt aaagaagagt ttacaaaaat gggatttgat gttgtagatt cacaatcaaa
    17941 tttcctttat gtagatatga aaacagatat acctaagtta tttgaagact tgaaaaaaag
    18001 agggtttgta ataagaccaa gtgctacaca tgcacgtgta agtattggaa ctatggaaga
    18061 aaataagaaa tttgtagcag ttttaaagga aatattaaat attaaaggag agccaagtat
    18121 tggataatag atagaaatat tatgaaacgg ggtgtagata tgaacttaaa aataaacgca
    18181 gagagatgta aaagttgtga atactgcgtt atatcttgta aaaaaggagc attaaaaata
    18241 tcaagtaaga taaataaaga aggttatgct catgtagaag ttgatgagag taaatgcata
    18301 ctttgtggaa tttgctatca ggtgtgtcca gataatgttt ttgaaataat taaagaagaa
    18361 gcatgtcaag aggcttagaa ggagttggga taatatgaaa aagatgtgga aggggaacca
    18421 cgccattgca gaagcagccc taagaggagg ttgtgaattt tatgcaggat atccaattac
    18481 accacaaaca gaggtaatgg aatttttatc acatagaatg tctgaacttg gaagaacttt
    18541 tatacagtcc gaaaatgaga tggctgctat atatatggta tatggagcat atgcaagtgg
    18601 aatgaggtct atgacgagtt cttcaggacc aggaatatct ttaaaacaag aagggatttc
    18661 ttacttatgt gcaaatcatt acccttgtgt aatcgtaaat gtacagagat gggggcctgg
    18721 tcttggctca ttggactcag cacagacaga ctatctaaga gatactagag ggggaggaaa
    18781 tggagattat catttgatag tttatgcacc aaacagtatt caagagactg tagatttgat
    18841 gtataactca tttgatgtag cggagaagta cagagtgcct gttgaaattt tgacagaagc
    18901 agctttaggt caaatgatgg aaccagtaga atttccagag tttaagaaga gagaaggaga
    18961 tttaggttgg acttatgatg gaagtaatag agaccatgca aaggttgcag ataaccaaaa
    19021 gccaactttt tgtatggaga aacgtatgag aataactgaa aatgaacaac aatgggaaga
    19081 ttatcaagta gaagatgcag aatatgtgtt tgtagcgttc ggtataccat caagaacaac
    19141 tatgaatgct gttagaagac ttagagaaca aggtgaaaaa gttggaataa taagaccaat
    19201 aacagtatgg cctttcccat ataaagcatt tgaaaaggta agtaaagatg taaaaggatt
    19261 tatctcagta gaatcaacag acacaggtca attagtagaa gatgttgctt tagcctcaaa
    19321 gaaagtatgt aaagaaaatg tacctgtgta tggtttattt agtggaaatc atataccaaa
    19381 aacattgcaa gtaatggaca cttatagcaa aataaaatct ggcgaaatca aggaggtgtt
    19441 ctaatatggc tacagaaaaa ttaacaccta gcgttgtttc aataccaaac aagttttgcc
    19501 caggttgtgg tcatggaata gtaaatcgta taatagcaga ggtaatagaa gaacatggat
    19561 atgaaaaaaa tcatgtatta acattaggag ttggttgtgt ctgcaatatg aattttagct
    19621 ggaatggtga taagatgcaa actgcacatg gtagagcttc atctacagct ataggagtta
    19681 aagtagcact tccagataca ttagtaatga cttaccaagg tgatggagat gcttatgtta
    19741 taggtctttc tgaaacttta aatacagcat atagaaatca taatgtgact gtatttgtaa
    19801 taaacaataa taattttgca atgactggtg gacaaatgtc atggaccact atgccgggtc
    19861 aagtcacaac aacaagtgta aatggaagag atataaagac tactggtagt ccactgaaag
    19921 ttcctgagat ggtggcaaac ttctttgatg tagcgtatgt agcaagaggt tcagttcata
    19981 gcccaaagga aataattaat ctaaaaaaat atataaaaaa tgcaatagaa gctcaattaa
    20041 atggagaggg atattcctta gttgaaatat tagcaccttg cccaactaac tggggtgtat
    20101 cactagagaa atctatcaaa tggatggaag aagaaatagt gccatattat gctcttggcg
    20161 aatttaagca aagggatggt gaatagttat ggaaaaagag ctagtatttg cagggtttgg
    20221 tggtcaagga gttttaactc ttggtctgat tgtagctgag tcagcacttg aattggggaa
    20281 acaagtaact tggatgcctg catatggtcc aactatgaga ggtggaaaag cctatagtgt
    20341 agtaaagttt tctgatgaga gtataggtgg gcctgatatg gaagaaatag atgttttagt
    20401 agcaatgaat aaaccatctt tagattatat aaatttagtt aaagaaggtg gaacagtagt
    20461 tattaataca tctgcaatag atgagaatgt aacattaaga gaagatataa gtgtagttaa
    20521 aataaattgt caggaattag ctcaaaaagt aaacaatcca aaagcagcaa atatagtagt
    20581 acttggagca ttaattgcta aaactgggtt atttgaaaaa gaattagctt taaaaactat
    20641 gtgtgatttc tttgaggaaa aaggaaaagg aaagtttaat gttcaaaatg aagcagcatt
    20701 catggaaggc tataataacg ccatttgata aattaccctt atcttaagtg atttatctca
    20761 agatttaata atctttagtg ccaagagaaa cttcatcttg agataaaatt cacttaaaat
    20821 cacaactata aacatatagg agcgtgaatt tatgagtttg gaaaaactta ttagaccaaa
    20881 aagtattgct atagtaggag tgactgataa acttggtttt ggaagaagtg cagcacttag
    20941 tatagtaaaa agtaaggaaa cagatagagt atattatgtt aatcccaaaa gagaagaatt
    21001 gtttgggaga aaatgttata agacgataca agaagttcca gaagtagtag actgtgtagt
    21061 agtatgtacg cctagaaatg tagttccatc tgtattaaaa gattctggag agttaggaac
    21121 aaaggcagta gtggtatatg ctagtggatt tgcagaggaa ggtacagagg aaggtacaga
    21181 tttagaaaat caacttatag aaatatcaaa tacatacgat atgaaaatat tgggtccaaa
    21241 ctgcatgggg ttgctaaatt gtatagataa ggttaatcta tgggcaggag gttctaaatg
    21301 ggatttagat actaaaaaac ctggtatagg aattgtaggt caaagtggat ttataactgc
    21361 tgaaattgtt tcaagtgatt attttaatat atcttatggt ttttcaacag gaaatggaaa
    21421 tatagttaca ctagaagatg cagtagattt ccttgtagat gatgattatg catcagtaat
    21481 agcaatttac cttgaaggtt taaaaaatcc tcaaaaattt atagatgctt taaaaagggc
    21541 agcacaaaaa agaaagccag taataatatt gaaatcagga aggtcagaaa aaggtgctat
    21601 ttctgctgct tctcatacag gaaatttagc tggttcgagt aaagcttttg aaagtatttt
    21661 tgaaaaatat ggagtaattt ctgtggaaaa tttagaacag tttatgtgtt tagcacaagc
    21721 ttttagtgtt ttagatggta atttacctgc aaattctaat tttgcagcaa taaacttctc
    21781 aggaggagaa aatactattt gtgcagattt agcagaagaa aatggtgtag aacttgcaga
    21841 aatatcttct gaaactaaag aagaaatgaa aaaatatctt ccaggatttg caacacctaa
    21901 aaatccacta gatgctacaa cagcattatt ccatgaaaag gatatgatag taggtttatt
    21961 acataccttt gaaaaggatg atagtgttgg atttactatg gtaggtgcaa atataagaga
    22021 tgaagaaaat gaaatgcatg aaaccctttg taaagctgta agtgaagcta gagaacaagg
    22081 attgaaaaaa cctgtgtttg cagttcctac acttgaagga aatagatatt tagattatag
    22141 gactagatta gaagataatc aagtgcctat aatgtcatca gttggaacta catttacttg
    22201 ttttaataaa atggctaagt ttatagatta tgattattca aaaagaactt tagaatttaa
    22261 agctgttaag aaaagagagt caaataatgt agtagcatta tcagaattag atagtaagat
    22321 tgagatgaaa aaatatggag taccagttcc aggacaaggt aatgcaaaaa gtattgatga
    22381 attagatgaa atcttaaaat atattaagta tcctgttgtt ttaaaaataa attcttctga
    22441 aatattgcac aaatctgatg ttggaggagt taaaatagga attaagaata gagatgaggc
    22501 agtagatgca tataatgaga ttttaactaa tgtaaaaaaa gcaaaaccag atgctaatat
    22561 tgatggtata ttagttcaag aaatggtaga aagtggtata gaaattatta ttggtataac
    22621 taatgatgac caatttggac caatgttatt agtcggttta ggaggagtat ttgtagaggt
    22681 atttaaagat actacattat atccattacc tataaatcat gatgaagcta ttatgatgct
    22741 taaaaaacta aaatcattta agcttttaaa tggctataga ggaagtgagc cttgcgatat
    22801 tgatgctctt gcagatatga tggtcaaatt aggaaaatat gcttatgaaa ataaggatga
    22861 agtcaaggaa atagacttaa atccagtgtt tgtatatcct aaaggaaaag gcgtatgtgc
    22921 agtagatgcc ttaatagtta aatataaata gtttataagt tttggtattc aaccataatt
    22981 aaagcaccga ctgaaaatca cattttgtaa gttagatagt gtgattttca gtcaccccaa
    23041 atttaattta aaaatatttt aaaatgaaag tatagtttta agtttcattg tgaaatagca
    23101 ctgtataaac aataaaatac tacgtataaa acgaataata aaaggtgttt taatgataag
    23161 aatcaatctt gattttcatt aaaacacctt ctttattata ttaaaaattt ttaaacgtta
    23221 tattgtcaca ggtgggtttg ttggagctgc atatgtacga ttagcaaatt cttggtcaac
    23281 aaagaataga ccaccctctc cggcaagttt aacacgtttt atattgtctt cacaatttgt
    23341 ctcttcttct gcttgttcag atataaacca aagtaagaat tgagaagttc taaagtctga
    23401 ttcagagttt gctacagatg ctaagttgtt tattaaagct gtaacctttt tttcatgagc
    23461 taacgtacgc tcaagaatat ccattgcaga agtaaagtct gaatctggca ttgggatagc
    23521 atcaagttga acctttcctc ctacattatg taagtatttg taaaaaaaca tagcgtggtc
    23581 tgtctcttct ttatattgaa cataaaacca attagaaaat cctttaagtc cttctgcttc
    23641 aaggtaagaa gacatagaaa gataaagata cgcagaatat aattcatggt taatctgctc
    23701 attaagtaat ttttccattt tagctgaaat catttttata cctccaaatt ttaaatgctc
    23761 ttataaaata taataatatt aaatggaaaa atattcaata aaagtgttta aatattttac
    23821 atatttaata aaaatttata ctaccattaa aaaaattata acttacaatg gctatagagc
    23881 caattaatgg tgcatttgct ccaaaatttg aatgccttac acttattttt ttatatttag
    23941 aaaaacttgc tgaagcagtc aattttttta ataatgtatt ttctataaat gtaccaggta
    24001 tattaaagtc atagcctatt attatagaac tacaatcaat caatgtaagt gtattaacta
    24061 atgcatatga tacgtatgta caaaattcat caagcaaaaa catggcaaga ctatctttat
    24121 tatttgcctc atctattaac ttgactaaag aaaaatcttt acagttaatt aatggggagt
    24181 taggataaat atggctcaat gattcaattc ttttaattag attactgaca cttgtataat
    24241 aatctaaaca tccattgtta ccacaactgc acaatggtcc actaaaatta attgatgtat
    24301 gtcctatttc tccactttgt cctaggttgc cagtatgaag tttattttct agaactagac
    24361 cagctccaat accattcatt atgtgtaggt atatataatt tgatatattt ttacctaaac
    24421 catacatttt ttctgctaat gcacccgaat tagcatcatt tatcaaaaat attggcaaat
    24481 cagacatttc ttttataaaa tctactatat ttaggtttga tacatttcca aaatctgggg
    24541 gatttagtat ggtaccattt atatcatcta ctggacctat tgaagaaata ccaattccaa
    24601 ttatatctct cttgttgatt ttcattattt tagtaaacat tctttttatt atatctttta
    24661 aaacatcatt tgatagacat ttagggtaag tttcagaaag ttgttttaca atattacctt
    24721 ttaaatctga aataattacc ttacatatat ttcttcttat taggattcca caaatacaag
    24781 gcgatatatt agaaatatct aataaaatgg gtttacgacc tgatgaatgt gcatttaaag
    24841 ttgatgactc tatttcccta atcatatttt cattaataag ttcagtaact agatttgata
    24901 aagtcatttt agtaagtcct gtagacttag ctaaatcaac tctggacata ggtgtatttg
    24961 tagaaagtaa tttcaatatt attaacttat ttttaatttt tacatctttg atgttagaac
    25021 cttgtttgtt actcatcata aatctcctta aataaagtta agtgttttat ttattttcaa
    25081 gtatactatc accttataat agagtaacat ttttttactt gaaaatatca ttaattagaa
    25141 tatatcacat tatatatcat aaaatatata aaaaaatgtt agaattaaat taaaagtgaa
    25201 aagtagattg atatataaat agtaaatact taattaaaaa aagataatga tagcaataat
    25261 tattgttacc aagaatctac tacttcaagt gttaccttag gtatttaagt tgatagtggt
    25321 ttgcacgtaa taaacttgat gtaatatata ttcttaataa tattgataaa agatttactt
    25381 aaaaataata tagattatgt aaacat
//
LOCUS       DAENCV010000049        25111 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAENCV010000049 DAENCV010000000
VERSION     DAENCV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.049"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..269)
                     /locus_tag="KQD82_002038"
     CDS             complement(<1..269)
                     /locus_tag="KQD82_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380508.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAI"
     gene            complement(552..848)
                     /locus_tag="KQD82_002039"
     CDS             complement(552..848)
                     /locus_tag="KQD82_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380509.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(867..1280)
                     /locus_tag="KQD82_002040"
     CDS             complement(867..1280)
                     /locus_tag="KQD82_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380510.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1264..1497)
                     /locus_tag="KQD82_002041"
     CDS             complement(1264..1497)
                     /locus_tag="KQD82_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380511.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1716..2090
                     /locus_tag="KQD82_002042"
     CDS             1716..2090
                     /locus_tag="KQD82_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380512.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            2702..3394
                     /locus_tag="KQD82_002043"
     CDS             2702..3394
                     /locus_tag="KQD82_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380513.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3590..5650
                     /locus_tag="KQD82_002044"
     CDS             3590..5650
                     /locus_tag="KQD82_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4380514.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5856..6179)
                     /locus_tag="KQD82_002045"
     CDS             complement(5856..6179)
                     /locus_tag="KQD82_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG4380515.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(6172..6873)
                     /locus_tag="KQD82_002046"
     CDS             complement(6172..6873)
                     /locus_tag="KQD82_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG4380516.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(7151..8536)
                     /locus_tag="KQD82_002047"
     CDS             complement(7151..8536)
                     /locus_tag="KQD82_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4380517.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(8982..9404)
                     /locus_tag="KQD82_002048"
     CDS             complement(8982..9404)
                     /locus_tag="KQD82_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG4380518.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            9882..12785
                     /locus_tag="KQD82_002049"
     CDS             9882..12785
                     /locus_tag="KQD82_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380519.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            12818..13264
                     /locus_tag="KQD82_002050"
     CDS             12818..13264
                     /locus_tag="KQD82_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380520.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            13387..13689
                     /locus_tag="KQD82_002051"
     CDS             13387..13689
                     /locus_tag="KQD82_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380521.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            13870..15276
                     /locus_tag="KQD82_002052"
     CDS             13870..15276
                     /locus_tag="KQD82_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG4380522.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            15620..16678
                     /locus_tag="KQD82_002053"
     CDS             15620..16678
                     /locus_tag="KQD82_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG4380523.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEM"
     gene            16777..17463
                     /gene="araD"
                     /locus_tag="KQD82_002054"
     CDS             16777..17463
                     /gene="araD"
                     /locus_tag="KQD82_002054"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG4380524.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            17481..18128
                     /locus_tag="KQD82_002055"
     CDS             17481..18128
                     /locus_tag="KQD82_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG4380525.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(18301..19668)
                     /locus_tag="KQD82_002056"
     CDS             complement(18301..19668)
                     /locus_tag="KQD82_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG4380526.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTISLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(19858..19946)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            20374..22494
                     /locus_tag="KQD82_002057"
     CDS             20374..22494
                     /locus_tag="KQD82_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG4380527.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            22598..23851
                     /locus_tag="KQD82_002058"
     CDS             22598..23851
                     /locus_tag="KQD82_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG4380528.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(24005..25066)
                     /gene="ytvI"
                     /locus_tag="KQD82_002059"
     CDS             complement(24005..25066)
                     /gene="ytvI"
                     /locus_tag="KQD82_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG4380529.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         9874 a         2891 c         3974 g         8372 t
ORIGIN      
        1 ataatggcta ctaatattat tgctattatt gtaatatcta atcttcttgt aaataagcct
       61 attgctatat actcaagacc tgttacatag cgataaagta cttctagttt aacagttttt
      121 ttcttatcta tatttccata tattgattct aatttattaa aatatttttt actacaaaaa
      181 gcagttttag gtttaattaa atatattatt gtagttccta ttataactat tattccaata
      241 agtataagat tgatatttcc aaaatccata gcagatacct cctattaaat ttgcttttct
      301 taatcaaagt atatcagtaa cacaagtaca aagagtatct aaaatattac aaatattaac
      361 aggatagata aaactatagt aaaaattgta ataataaagc gaacgaaaat tatcatacta
      421 aaacataaga catgttataa ttgtattaga tagaagatta acatacacca aagcactcct
      481 tataataaag agtgcttatt tttttgaaat tcatcaatat ataaactatc aagaacctta
      541 ttcaacacac cttaaaatcg atttaaatag ctctttttga taacataatt tatatgatat
      601 aataaaaaaa tagaagtgta ccaccaatac acttctatag ctataactaa gcattctctt
      661 gctgggggag tgcttttttc atttctccaa acaacattat ttacttcatc agtaattatc
      721 aaatcaatct cattatcttt caaaatctct gttgcagtac aaattagatt atctaagtca
      781 aaacttcgag ttatctctag ttgtttgtta tttacttctc tttttagtat ataagtttta
      841 aaatacattt atatcacccc ttgtatttac tcttttttct ctaaaaatat attgataagt
      901 aaatatgcaa caaataaaaa aatctcaatg acacatagtg ttatagctat aactagaagt
      961 gattgatttt tccatgcact ataagctatg atgaaaagaa atgtaaaagt aaataatgta
     1021 taattcataa ttttaactaa gatatgactt gttttcattg ttaagtacaa gtctctttca
     1081 tctgtactat cgtgtaattc ttcaacaatg ccttttcttg aaaaagcatt acaaaaatta
     1141 aagattgcta ttacaattaa aagaaatgat gaaattagga atcttttatc ttttaaaatg
     1201 caataaataa ctaaactagc acaacataac acacaaacta taatacccac tataaaactt
     1261 cgtttactct ttactttcat gttttttatc ctccatttct ttattttctt ttaaacaaca
     1321 tagttcttca atcgttgtac caaacacttg tgccatacga taggcaagca taagagaagg
     1381 gctatattgc tctttttcaa tagaaataat tgttctgttt gatacatgga ctaaatcagc
     1441 gagttgttgc tgtgttagac ccatatttgt tcgaaattct cgtacttttg ttttcattcc
     1501 ataacctctt tctatatgaa gctaacttca tgtgaagtta acttcataat atcctaaaat
     1561 gagtaaaatg tcaatctcat tagataagta ttataaaacc taaaagaata atatggtaaa
     1621 aattggatgg aattatttac cttaattctc ttgtttgtaa aactttattg atatagaatt
     1681 taacatatac ataaatcggc aaaggggcgc gaattatgaa aattagtaaa ttaccagaag
     1741 cggaattaaa agttatgaga tatatttgtg aagctaacaa ggtattaact tctagggaat
     1801 tagttgctgc aatggaagaa aaatatgagt ggaatgaatc aactacattt acagttttga
     1861 aaagattaga gcgaagggag tttttaagta cagagaaaat aggcaaactt acacattata
     1921 atatactagt aaaagagaaa aaatatctaa ggtttgaaac aaaggaattt ttgaagaaca
     1981 tacataaaaa ttcaatttca agtttaatgt cagcgttaca tggtgaagac gacgaagtgg
     2041 atgaagataa attaagtgaa ctcgaagaat gttttaagaa tttgaaataa gaaaatgaca
     2101 aattgataca taataaataa aatgaaggta tgaaattata actttttcta ccttcatttt
     2161 gtttattatt ctgaacatgt taagaaaatg aaataagttt gtattttagt tatataaatt
     2221 ttaatagaat atgtacagta tatagtagat agaatgtata aaaatagaat ttaaataagt
     2281 agctattatt gcagtgaaat gaatgtttct aagtatattt gggtggaaga atggtggaag
     2341 aaaattagtg aaatgtaatt cttccaccat tcttccacct agaaaataga aactgtttaa
     2401 taagaattaa tatacattaa ttcagctgaa tgcaatcaaa atacttaaat gaaagtaatc
     2461 tgaaaacaac ataattcaaa tcaatttatt caaataaacc tcaatttatc ttaaatctta
     2521 agaaatattt aagaattagg ataatatttt cttaagattt atattttatt atatagttat
     2581 aagggaaagt tacaaaaatg taagccaaag taatataaca atttagtcat tgttgagaaa
     2641 gggagtgaaa gatattgtat aatatagata agtattatta tacaatagag gaagtgaaaa
     2701 tatgtacaat atattagtag tagacgatga taaagaaatt gttgattcga tagaaatata
     2761 tcttaaatca gaaggattta aaatatataa agcatatgat ggaatgaaag ctttggaaat
     2821 aataacagag aatgacattc atctaatact aatggatata atgatgccta aattagatgg
     2881 aatcaaagca actgtaaaaa tacgagaaga aagaaatatt ccaataattt taatttcagc
     2941 aaaaagtgaa gatacagata aaataatagg attaaatatt ggagctgatg attatataac
     3001 aaagccattt aatctattag agcttatagc tagggttaaa tcaaacttaa gacgatatgt
     3061 aactttaggt aattatgaag atacatgtaa agaaatttta aaaagtggag ctttggagtt
     3121 aaatacacta acaaaagaag ttaaaattga tggtgaaaac ataaaaatga ctccaattga
     3181 gtataaaata attgaacttc ttttagaaaa taaaggacga gttttctcaa tagatgagat
     3241 atacgaaaaa gtatggaatg aggaaagttt taattcagaa aatacagtag cagtacatat
     3301 cagacgaata agagaaaaga ttgaaataaa tccaaaagaa ccaagatttt taaaggtggt
     3361 gtggggagtt ggttataaga ttgaaaaatt ttagtggaaa aattataaaa gaaaagagtt
     3421 taatatttaa ttgcattttg attttagtca tttttacatt tacacaaatg ttaggaattt
     3481 taatatgttt taattttttc tctgagagtt taatttactc tatggggatt atgcttttct
     3541 taaactttat tttatataaa atactgacaa tgtagatgag gtgggcaact tggagaaata
     3601 tagagtatta aagttagttt cattaatcat aatatctatc tcatttccat taactatatt
     3661 gttggtgaca ataggacttg ctgagcctaa atacaatata caaaaaactt ctacaaagag
     3721 tgggattttt gatatatctg atgattttta tgatagtaat atatttgacg aaggaacaat
     3781 ggcaagtcta ttacccaagt ttaagctatc tgtaggtggt aaattaacta gtaaagaaaa
     3841 aggtaaattc caacaagaag gtaaaagtat agatttaagt agtagtgatt ctgagtatga
     3901 taaaatcatg attaatgaac aagcttctga agaatattta agttatctaa aaaatataaa
     3961 agtgtatgtt attaatacat acacaggagt attttataca aatacgtcat atgctagttt
     4021 atatgatttt aaacaaaaaa ctaagagcta ttgtaatgta gagataataa ataagatagg
     4081 aaagagctca tatattaaaa caataaatgg agaaaaattt gaactgaata atctgagtct
     4141 ggaactaaat gaagtagatg aaggttttga ggcatatgta tcttttccag aagagccaac
     4201 tatagaagat ggaatagtat atactaattt tcaaatattt aaacaagcta cagaaaaagt
     4261 aagattattt tttgtagcta gtttaagttt agcaattata tgtttattat ctataatttt
     4321 atatatcaaa ataaaaggtg aagttttaaa aagaaataat gtatttttac taatatacag
     4381 taagatacct atggaattta atgtaatatt agtatctatt tcaattgcaa ttttaactat
     4441 atatgctctt gctgtatctt atcatattga tgtaattgca ttagtttctt tatctttact
     4501 gatagtatca aataatatat tttttataaa tgtgcaattg agttatttag aaaataaaat
     4561 gtgtatattt aagacaagta ttatagtaag agtgatttta tggttaataa aaactgtaag
     4621 ggaggtcagt aacgctataa agaaagtatc tttagcaagg aaagttattg ttatagtgat
     4681 aatatcttta gctttaatca tggtaattag cactcgaagt ataggtatgg gattactgtt
     4741 ttctatatgt atttttttag gatttacaat atatataact aaaagacttt catatcttag
     4801 ttacgtaata gaaggtacag aaagaataaa aaagggagaa ttagattata agataaaaat
     4861 tactgggaat gataatttta cgtctttagc tgaaaatata aataatattg gagaaggtct
     4921 tgatagagct atagaagagc aagtaaaaag tgaaagaatg aagtctgacc ttataacaaa
     4981 tgtatctcat gatttgaaaa ctccacttac ttctataata aattatgtag atttaattaa
     5041 aaaagaagaa tcaatacaac cagagtatat aaatgattat ataaatgtac ttgagtcaaa
     5101 gtcaaaaaga ctaaaattat tgatagaaga tttatttgaa gcgagtagag taagtagtgg
     5161 caatatagaa ttacaaatta gcaagattga tttagtgcaa ctattaagac aaagtatagg
     5221 tgaacttgaa gaaaaattat ctaaaaataa tttatattta aaattaaatg ttccaaatga
     5281 taaggtgtat atatgggcag atggaagaag aatgtataga gtatttgaaa atcttttatc
     5341 taatatagca aaatattcct tagaagaaac tagagtctat attgatgtat atgactatgg
     5401 tgaaaatgta aaggtaacta tgaaaaatat atcttcttat gaattaaatt ttgacccaag
     5461 tgagattatg gaaagattta aaagagctga tgaatcaagg aatactgaag gaagtggatt
     5521 agggcttgct attgcaagag atttagtagc tttacaaggt ggaaaatttt atatagaaat
     5581 agatggagat ttatttaaag caaatttaga gtttcaaaaa tatgaagaag aaataacaaa
     5641 tatggaatga gatagtattt aagtatatat atttttttta ctgtcgaaag cttttattat
     5701 aatatattag aatatttaca gtagagaatg aagagtaaaa acagagtaac tttaagtcac
     5761 tctgtttttt atttaaaatc aataatataa caacttaaat taaaattgta cacatagttt
     5821 taaaataact tatacaataa aacattatcc aaatatcaca agataattcc aaataaatat
     5881 atagatatca ctcctgtaat aatagaaata ccaatagatt taaacttata tgcaacaaga
     5941 agtacaatca taaaagatat aattttaggg ttagctatag ataaatatag actatctcca
     6001 ttaaaaaata catctttagc agttaaggaa gtaagtattg ctacaggtat aaatttcatg
     6061 atttttttca aaaaatcagg catctctttt tttgaaaaaa ttaacatagg aagcactctt
     6121 ggtagatatg taacaatgta cattccaatt ataataaaaa atatataatt attattcata
     6181 agattcctcc tttttacagt aagattcttc tataaagaaa ccaattaaag atgctaaaat
     6241 tgatgcaact attattacta aattgctttt gaataatgca tataaaataa cagaaataat
     6301 gattgatgat ataaatacta caatataaat cttatttttt atttgagaaa ctattaaagc
     6361 tataaacata gaagtcaata tgaagccaga aatagaactg tctatagata tactttctcc
     6421 taaaaatgct ccaataaaat tagctaaaac tgatgaaatt aagcagaaga aattaagata
     6481 aatagcattt ctatcagacc aatttccagc agtaaatttt tcataattaa ctgcaaaagt
     6541 ttcatctgtt atagtatgac aaaatggcaa tataaatttt ggacttttat tttttatgta
     6601 catgttcaaa ttagaactca ttagaaaatg tcttaagtta actataaatg tagtcaaaac
     6661 aatagatgtt acacttgcac cagtagaaat cattgaagct gcaatgaatt gagaacttcc
     6721 tccaaatact aaagttacca ttagtgctat ttgaaaaggt gaaagaccag atttttgtaa
     6781 tagtattcct cctactatac ctataggtat ataacctaag aatataggaa ttgaagcaat
     6841 aaagctttct tttaattgtt gagttctttc cacaataaat cactcctttt gtattaaatg
     6901 tattaaagta ttcgtaaaac agaaaaatag attaattgta taattgcaat tataattaaa
     6961 aataaatttg gattaatata taaaatacta tacaacaaat agataatctt aaacggttat
     7021 taaattttat ctatttgtta ttatgatgta ataatacata ttaatctcta taagttaatt
     7081 actatctggt gtaattttat tttaacaaca aaaaaaaata cacacaatta aaatattgtg
     7141 tgcaatacaa ttattttttc tcttctaata gtctttttaa tacgctcaaa ccagctttaa
     7201 gttcttctgt actttctgta gcacatacag ctattcttat tgcagaaatt ggtttttctt
     7261 ttccaacagc aaaacgttca gaagcataga cctgtacacc ttctttaagt gcttgtatct
     7321 caaatttttc tgccattatt ccatcaggta gaatcaacca tctaaatata cattcttcat
     7381 ttcccaaaag attatatcca gataaatatt gattaataat cttgtttctt cttcttgcag
     7441 atttacggcg tgcattaatt aatttatcgg cttcacctga tgtaatcatg cgataagcaa
     7501 tttctgttaa aaaataagat tgagatagat taatgttgta taatgcgctt gataatgcct
     7561 ctcgatactg tttaggtgta gagatatatg ctagccgtaa actaggtgca ataatttttg
     7621 ataaactcgt aatataaatt gtttgattgg gcagatagga agccactgga tttaaatgtg
     7681 tttcattaag taaactatga atagcatcct caataacaat taaattgtat ttagatgcaa
     7741 tatttgcaat catttttcgt ccattttgtg acattatatg tgtagttgga ttttgataat
     7801 ctggtattat gtatagacct ttaatatttt catttttaca agcatataat agtccttctt
     7861 cactaatctc attatcttct tgtttaattg gaattagttg aatcccaagc attttagctg
     7921 ctgtctttat tccaggataa gttaatgggt ctacacctat tctatctcca tgttgaaaca
     7981 aaccagctaa aatagcaaca attgcatttt gtccaccact tgcaggaagt aagctatctg
     8041 atgtagtttc aagcccagct ttataaatta atttggcaat agcttctttt tgccatttag
     8101 caccatctct ttgtccatat tggaatatag ttttaaaatc tatttcctta attatgtgtt
     8161 taaacaaagt attcatttct tctagcgtga aatctggatt cattgtgccc atttcaataa
     8221 atgtaatttt attattactg gacatcaaga atagatttga acgtgtatca taagatacaa
     8281 aagttccact tcctgtaact ccacttatta gacctttttt ttcacaaatt ttaaaagctc
     8341 tagagatagt actcacatta acatctaaga aatctgccaa ttcacgttgg ggtggtaatt
     8401 tagtacctgg taataaaaat ccattgttga tatcttgttc tagttgttct gcaagagcct
     8461 tatataatat ttgacctttt gaaggtctct ttggtttcca gttcatagga taattttcaa
     8521 aagaattaat tggcataagt tttcacctca cattattaat ttgatttttt ctttatctaa
     8581 aacaataaat tctaaagata caaataaaaa ctgataggaa ataattctat cagtttttgt
     8641 cttttaattg atttcaaatc ttcctataat ttttatacct tctttatcat aaagagggat
     8701 tgttttttta aatatattaa aatttctttt tcttgtatat aaaacagctt cttctggtgt
     8761 ctttggttga ttatcacaat cgtacaaatt tctttttttt aactaaaagc aataatacat
     8821 aaaattatta ttgctaatga tactcttaat gtggtatata gtcatagtgt cacatgtcta
     8881 tcaatagttt tttcagtaat tattgtaacg aatagtagga ctcataaaat acttagaaat
     8941 atgtttttag ataaatttta aatatataat ctaacttata tctataaatt aacactatta
     9001 ttctccaatt ccatttctag tttttttata aatttattaa attcttctgg ttgccttttt
     9061 ccaaacaata ctcttacaag agctgaattg atttttccaa atatattcct cttgctctca
     9121 ctttcaataa aaataagttt tgtattttct aagtcaatat gtactaattc atatctagta
     9181 atagtacttt ctcttttatt gcttgttttt atctcatata ctctattttt ttcgaaatta
     9241 gttatttcaa ccttaaatct tttattattt ttttgctgta caaatgttcc tactgctttt
     9301 ttctcattaa aattaggaaa atttagtctt gcagattttg taaatatatt aaatatgtct
     9361 tttgcagaat attttaataa aacttcagtt ttaaatgtat tcatatcgtt ttcccttctg
     9421 tatataaatt taatatttat aagttaattt ttaagttaaa atagtataat atgccatatt
     9481 aaatcatgtt aaaactattt atatagctaa aatcttacct atataagtat gtatactatc
     9541 atataaaaat aaaatcttaa aatagaaagg gttaaaaaca cagtttatta taatttttat
     9601 tgttgagtag atacgtacta ttatatatga ttgagcaagt ttttcaatca taaatttata
     9661 aagtattttg cattaaaatt taaacaaaga attaaattat ttaagattat gaataactaa
     9721 tacatttagt aaattactaa atatacaaaa tgtaaatact tatcaaaatg tataaatact
     9781 tatgacatct agttacttac agggtttaag tagaaaaaaa tatcaaagta agtatggcat
     9841 gatagttgct tatatattta tcaagaaagg agttattaag catggagttg agagataaac
     9901 ttgtaaacat cataaataca gaaaataaaa aagatccatt aacagatgtt caaatagcaa
     9961 agtttttaag tacaactaga gagaacataa ctaatttgag aaaagaactt aatataagta
    10021 attcaaggca aagaagatat ccttatttaa aatcagcaat ttcggcaata ttacaaaaga
    10081 ataagaatat ttctatatca gaaattacaa gggaattgat gacagaaggc tttaatatat
    10141 ctagaagagt tgtggaggaa cttttaccta aagaaagttt agaaatggat gtagtagagg
    10201 aatctgaaga agaatcgaat gacccatttg aaactctgat aggtaataaa gggagtctta
    10261 gaaatgctgt agagcaggct aaatctgcta tattgtatcc accaaaaggg ttaccaacgt
    10321 tgattattgg ggaaagtgga gttggtaaga gtttattttc tagacatatg tatgagtttg
    10381 caaagcaaaa aaagatagta aaagaaagct ccaattttgt tgtatttaac tgtgctgatt
    10441 atagtgacaa tccacaatta ttattatctt tgttatttgg atataaaaaa ggtgcattta
    10501 caggtgctga atatgataca ccaggtcttg tagaagaagc tgatgaagga gttttatttt
    10561 tggatgaaat acatagattg cctccaaaag gccaagaaat actattttct attttagata
    10621 ggggaaagtt taggagatta ggtgaaacta atgctgaaag aaaagtaagt attatgttga
    10681 ttggggcaac tactgaaaat gtagagacta atttattatt aacattcaga agaaggatac
    10741 caatgctaat aactttgcca ccattacatg atagattact aaaagagaaa atagacctta
    10801 tatacaatat ctttcaacaa gagtgtaata gaataaatgc aaaaatattt gtagataaaa
    10861 atgtgattga aatacttgca ctaaaaaagt ttagtggaaa tataggacaa ttacaaaata
    10921 tgattcaggt tctttgtgca agagctttta tgaagtttat aaattccaaa gatgaagata
    10981 gtgaatcgat agtagtagta gatataaatg aagttttaaa gcttaaagat tcttttaaag
    11041 atgctgcctt tcaagagatt gaatatacag agataagaaa atatttgaaa aatgctatat
    11101 ttataccatt taatttagaa gaatcatcag taaaaggaag tcttttaagt gatgaatata
    11161 tattaccaga ggacatatac aaaacaattg aaaagaaata ttatgaccta aaatccctag
    11221 acattgaaga tatagatata gagaatattt tatggacttt tattttaaat agatttgctg
    11281 acttaaagtt taatctcgat agtaatgaag aaattttttc aatgagtgat ttaaatagtt
    11341 ttgtaagtga tagtttagtt aaactggtaa aagaacttat gaatgatata gttgaaaaaa
    11401 atatgaattt agaagtaaat aaaaatatgt tcaaatatct agcaatacac ttagaagaag
    11461 ctataaaaag aattaagcta aatcaaaaga taataaatgt aaatttagaa aaaataaaga
    11521 ttgacttttc aaaagaatat gaaattagta aaagttttgc aataaaaatt gaggaatcaa
    11581 ttggtattaa gattccagat gatgaaattg gatttataac actctacata aagtctgctc
    11641 taaaaaatga agtgaaaaaa gataaagtag ggttaataat aatttctcat ggacgtatag
    11701 ctacagagac tgtaaatgta gttaaagaac ttttaggtgt aaagtttcct gtggctatag
    11761 atatgccttt agatgaaaaa ccaataaata tatacaataa ggcagtagaa ctgtcaaaaa
    11821 taatagacca agggaaaggc atattgtttt tggtggatat tggttcactt acaaacatag
    11881 gtcaaatagt aaataagaga accaatataa gcactaaaac tattgataga gtagacctcc
    11941 taatggcact agaagcaact agaaaagttt ctatgggaga ggaagaactt gatgaaatat
    12001 ttttttcttt gctaaaagat aggatgggat ataactatga attagaaaaa cacatagata
    12061 aatcaaatgc aattataaca ttatgtctta ctggagaagg aactgctaag tatataagta
    12121 aaacgttgga agaaaagtat gataacacta aatgttatca aatgagtgca ttggatgaaa
    12181 atttattttg taaaattgaa aatctaaaag agacaaacaa tatattagct attgttggga
    12241 caataaaccc taagatacca ggtataaatt ttatacccta taataaagaa cttttcaaaa
    12301 atttggatat ctatctatca aaatcaaaac aacaagatga cagaccaatt aaatcgtatg
    12361 aaagaatgct tgatgaggat ttagtgattt ttgaaccaga tatatatttt aaaaaggatt
    12421 tattagaata tgtatgttca attcttataa ataaagaata tgtagaaaaa gattacttag
    12481 attctgtttt acatagagaa gaaatgcttc caacatattc taaaggagct ataggtgtgc
    12541 ctcatggaga ttcttctact gtaaatagta caaggtttgt ttttgttaaa ctaaagaatc
    12601 ctattgattg gggtgtaggt aatgtaaatt tcatatttat gccagttttt caggctaatg
    12661 ataaagagat tgttaaaaat gttcttgaga tactaaaaga tgcagaattt atgaacaatg
    12721 ccaataagtg ttttgataaa gatgctttta gaaggattat atttgataaa tttaaacatc
    12781 tgtagccata gcaaaaaaat tgagaggagt tagagtaatg aatgtagatt taatatcatt
    12841 gggtttagaa tctaatgatt atgaaagtgt tattgaagaa ttaggaagta taatgtgtaa
    12901 aaaagaatat gtaaaagaaa catacataaa tgctgtcttg gaaagagaaa ggacattacc
    12961 aacaggtcta gatattggag aaatgtgtgt tgctattcca catacagatt caaagcatgt
    13021 aaatgagtct aatgtagctg tagggatatt gaaaaatcct gtaaagttta attcaatgat
    13081 agacccaaaa gatagacttg atgtagaact tgtattttta ttagcagtaa agaacccaga
    13141 ttcacaagta aaactgttaa aggatttaat gtcagttttt caaaatataa agttattaaa
    13201 aaatataaaa aacgcatcaa caaaagaaga agtagctaaa ttattggact ttatagaaat
    13261 ttaatgtagg agaattttat aaataaaaaa ttaaaattta aacaatcaaa gtaagtatta
    13321 tttttttaat aggtttaatc ttatataaaa taaaaattta gagtgaaaaa ttaggaggaa
    13381 ataattatga aacaatttaa tgtattatca gtttgtggtt cagggacagt tacatcatct
    13441 atggtagcag gtaaattaaa agaagtacta ggtaataagg ggatttctat tactacaaca
    13501 gaagctagac caacagaagc actaaattta gcacaatcag gaagatttga ctttataaca
    13561 tttacgagtc cacttcaaac aggcgattat ggaataccaa ctataaatgc atttgcttgc
    13621 ctaactggta taggggaaga ggcattcttt gaagaagttt tgactgttat tgaagggata
    13681 gataagtaag ttttggagac gtgaatttat agtatttaaa tcaatgactt agatactata
    13741 ataagctaat attaactaaa tatttgtgga aaaaacattt tatatgttgt cataaattaa
    13801 aaaattataa aaatagtcac ttaagtatta aattaagctt ataaatattt ataagtagga
    13861 gggaaaaaga tgttagaatt tatgaaatct ataatcgata catttggttc tgcaattata
    13921 gttccaatta taatattcat aatcgcaaag atatttaaag taactactaa gaaatcgttt
    13981 ctttctgctg tgtatgcagg agttgcttta caaggtttta cactgatttt aaattcattt
    14041 acaccaataa taacaccagt tatcaatagg atggttgaga gtacaggagt aaacctacca
    14101 gtttttgatg ttggttggca agcaacatca ttggttgcat tttcaacaag tgcaggtatg
    14161 atatatttag gtcttggtat attattacaa acaattttat ttttgataaa atggacagac
    14221 gtgtttcaac catcagattt atggaataac tattcttata tggtatgggg agcaatggtt
    14281 ataggagtaa caggaaattt tcctttaggt atagcatgta tggttttatt aaacctatat
    14341 agtttattaa tatcagagtt agttgcaaag agatggtcta gttactatag atatcctaat
    14401 tgtacaataa tagctatgca taatgttgaa gcttctgtgt ttgcagtatt tgcagaccct
    14461 atatacaata aattgggatt aaataaaata aaattaaatc caaaagaatt agagaagaaa
    14521 ttagggtttt taggtgaacc aattacttta ggtctttttt taggtatgtt tataggtata
    14581 ttaggtaata tgacgagaat aaatactatg gaagcttggg gagaaataat gaaagtaggt
    14641 atatctacaa gtgcagtgat ggcaattttc cctaaagtag caagtatgtt tgctcaagca
    14701 tttgcaccta taacagaagc agctagaaag attatgcaaa aagctggaaa ccgtgagtgg
    14761 tacatagcag taaatgatgc tgttggatat ggtgaacctg caactttaat atcaggtctt
    14821 atattaattc caataatgct tgtaatagct atggtacttc ctggtaataa ggtattacca
    14881 gttgtggatt tattggcaat tccatatatg gttcaaggtt tagttgccat acataatggt
    14941 aatataccaa aagtattagt ttcaggtata atttggtttg gattaggatt atatgtttgt
    15001 acatctacag caccattatt tacagatatg gctacaaata taggtgtagc tatacctgca
    15061 ggagcaatgt taataacaag ctttaatata ctaggaaaac ctttgatggg attagttttc
    15121 tttgcatttc taagtgcaaa tccaatttat ataggtcttt cagttgtaat ttactttgtt
    15181 ctatgggcat tgtttagaaa gaacaaaaca agtattcttg attatttaga aaaacaagca
    15241 ctcaaaaatg tagaagaaga gcctgtagca gtttaagttt ataaaaatga aataaaaata
    15301 attaaaggag tttataattg attttatata agtatcagat aatacctgag aaatatttaa
    15361 atgaaatata gacttctttt tatttgataa tatttgtaat tataatgcat tgtattttta
    15421 tagttgagtt ttctgtataa cctgctattt aaaacatact cttttatatt tatacagtaa
    15481 tgtggtttaa ttttataaaa tgtaatctct actggtaaaa gactttgtat ataaggcatt
    15541 tgcctgaata aaccatttat cttttgtatg gcatagatat tgctatatag aagtatatag
    15601 tatgaaagga gatatttata tgggagataa aatgagagca tcagtattgt ataatgtagg
    15661 tgatgtcaga tatgaaatgg tagatatacc agaaataaca gatacgcaag tgcttgtaaa
    15721 tgtaaaatat gttgggatat gtggttcaga tttacctagg tctatggtaa gtggtttgag
    15781 tggaaataca aaatatccat tgattttagg gcatgaattt tctggtgaag ttgttaaaat
    15841 tggagaaaaa gtaaagcata ttaatgtagg agatagagta gctgttgcac cattagtacc
    15901 ttgtggaaaa tgtgattatt gtaatgaagg taattttggc ttgtgtgatg attacaatat
    15961 aattggaaca agagtaaatg gagcgtttgc agaatatgta agagttccag aagaacatat
    16021 tttaaaacta ccagatacat tggattatga aactgctgcc ggaattgaac cagcaactat
    16081 agcttatcat gggatttcaa agtctaatat tagagtagga gatagtgtag ttgtgttagg
    16141 atgtggtcca ataggtcagt ttgttataca atgggcaaag gtgtttggtg catctaagat
    16201 tatagcagtt gatatttttg atgaaaaatt agaactttct aaacttctag gagcaaacta
    16261 tatacttaac tcaaaagaag taaatgtaat caaagaaata aaaaaaataa caaatggtgg
    16321 tgctgatgtt gtaatagaaa ctgctggtag ccgatttaca caagaacaat ctctatttgt
    16381 agcaaaaaag agaggaaata tagtgtttgt aggtatatca cacacagaat taccacttag
    16441 tgcagacgct actgagtgta tattacgtgg agaacttacc ttgaaaggtt cttggaattc
    16501 atatacaagc ccttatcctg gaagagcatg gactgcaaca ttggatttta tggaaaaagg
    16561 tgacattatt tttaaaccca tgatttcaga taaaataggt cttaatgaag tgggtgactt
    16621 tttaagtaaa atgagcaaga gagaaattaa tttcaataaa atcctagtag aaatgtaaaa
    16681 atttaataaa aaattctaaa agcaattaaa ttttataact ataacattaa ataaaataaa
    16741 aataattttc ttaaataaaa tataggagga agaaaagtgt tagaaaattt aaaagaagaa
    16801 gtattaaaag caaacttaga attacctaag aaaaagctaa ttacttatac ttggggaaat
    16861 gtaagtggga tagatagaga aaaaggatta gtggtgataa agccaagtgg tgtagagtat
    16921 agtaatatga ctgtagaaga tatggttgta gttgatttag agggaaatat agttgatggc
    16981 aaattaagac cttcttcaga taccccgact catctagttt tatataaaga gtttgaagaa
    17041 ttggggggaa tagtacatac acattcaagt tgggcaacaa tttgggctca aataggaaaa
    17101 tctataccat cttgtggtac tactcatgca gattattttt atggaagtat accatgtacc
    17161 agaaaaatga ctagagaaga aatttgcaat gaatacgaaa aggaaactgg aaatgtaatc
    17221 attgaagaat ttatgggaag aaatccaatt cattgtccag gaataattgt aaatgaccac
    17281 ggacctttta catggggaaa agatgcaaat gaggctgtac acaatgcagt agttttagaa
    17341 gaagtcgcaa agatggctta ttatacagag ttaatgagtc ctgataatat tatggataag
    17401 gttcttatga ataaacattt ttcaagaaaa catggaaaaa atgcatacta tggtcaaaaa
    17461 tgattatctg gaggtgaatt atgctagact ctttaaaagg taaagttgtg gaaatagcaa
    17521 ttaaagcaga gcattatggt ttatgtaaag aaaagactgg taattttagt attagagata
    17581 aggatactgg atatatactt ataaccccat caggtgtagg aagagaagaa ttaaaagttg
    17641 aacatatttg tgtattggat ttggatggaa atgtaataga agttgagaaa ggtattaagc
    17701 caagtagtga ggtattgatg catattgaaa tatacaaaac aagagaaaat ataaatgcca
    17761 tatcacatac tcattcttta tatgcaacag cattttcagt agcaaagaaa ccaattatac
    17821 caattgtata tgaatcagtt aattatggcg gttatgtaga tattgcttca tacgaaagac
    17881 caggaacttt aaaattagct aagagtgttt caaaattact gatagataca gatgcatgct
    17941 tattagaaag acatgggttg acaactgtag gtaatgattt agacgaagca ttattaaaat
    18001 caagatatgt ggaagaggta gcagaaatat actataggtc tttagtatta aatcaattta
    18061 aagaacttga agtagtagat gttgatgagt ttaaatcttg gaaataccca gaagaaataa
    18121 agttttaatc taaataattt tcattgaagt ggttaattaa aatatatgta ttatttatat
    18181 caattactat gttttattat attaattacc gtattttgta aatatattta ttacattttt
    18241 tcaaaaaaat atagaatcca aaagccatat aaaattgact tgggattcta tactttttat
    18301 ttatgcattt ttctttatct gattttctgg agaatttata tgttcaaaat attttttagt
    18361 ttcagatact attataggtg ataatgcaag taaagctata aggttaggta ttgccataag
    18421 cccatttaca atatcagcaa ttatccaaac aacttctaac tttaagaaag cacctaataa
    18481 caccatagcc acaaatacag ttctataccc attcatgcct tttacaccaa acagaaattc
    18541 aaaacatctt tctccataat aactccatcc caaaattgta gtaaatgcaa ataatgttaa
    18601 actaattgta agtaatattg gtccaaatgt aggaattgct ccgttaaaag ctgcttgtgt
    18661 cataacagct ccatttaggt ctgaacacca cactcctgaa ataacaagag agaatccagt
    18721 aagagtacag attataatag tatctataaa agtacctgtc atagatataa gaccttgttc
    18781 cgctggccat tcagttttag cagcagctgc agctattgga gcactaccta gaccagactc
    18841 atttgaaaat acaccacgag cgataccatt tctaattgcc agtgacatag ttgctcctaa
    18901 aaaaccacca gtagcagcag taggtgtaaa tgcactttta attattaaca taaaagtttc
    18961 agggatttta ttccaaaaag atattaaaat gattccacag attacaacat atataactgc
    19021 cataaaaggt actattttag tagctacatt agatatactt ttaagtcctc caaatacaac
    19081 taaagctaca aatactgcta atacaacccc tgtaattcta gggtctatcc cgaaactatt
    19141 atttattgca tctgttattg aatttacttg agtaaaagta ccgattccta aaagtgcaac
    19201 tagtatacca cttatagcga aaaatattgc aagaggtctc catttttctc ccataccatt
    19261 tactatgtaa tacattggcc caccagacac ttgaccattt ttatctttag ttctgtattt
    19321 tattgcaaga acaccttctg aatacttagt agccattcca aagaatgctg ctatccacat
    19381 ccaaaaaagt gcaccaggtc caccagcttt gatagctgta gcaacaccaa caatattacc
    19441 agtaccaact gtagcagcta gagcagtaca taaggctgca aaacttgaaa catcaccact
    19501 acctttgttt tcagcagaga atattaactt aagagctctt ggcagtttag ttatttgaac
    19561 aactccaagc ttaacagtaa gtagtatacc agtaccaact aatagtacca gtaggggtgg
    19621 tccccaaata aaagcatcaa ctttgtttaa aatatcaatt agattcataa aaacactccc
    19681 tcattttttt tgctttacat aatcaataga atttttaatg aagtaattga caggaaataa
    19741 gtataattta ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc
    19801 tctggatgta cactaattag caaccaaaat aaaagtatta gtaaatactg tttttggtag
    19861 ataatccctg tccttttgcc tgagagattg agcataaaag ccttgcccct tcggcgccca
    19921 taatgggtct ctccagagtt ccgtaaattt tacagtccta cttttaaaag tacctgagag
    19981 tgttactcct tcggtgtgct atatagcaac tcttctataa aatcttaacc ggatattgat
    20041 ttgtcatagc ttattgtatt aataatatta atataagttt ttatgttttg tcaactaaaa
    20101 aattaaaaaa caaagaaaaa atataaaaat gtaagcctat ttacgaatta taaacttaga
    20161 aagatgagta atatacgtga aaatgaattt tgtaatacac atacaaatga agtgtaaaat
    20221 aagttttaaa taaaaaaata ataaaatctt aaataattaa ttagatatga ttaaattatg
    20281 gtaatattat agttacgata ttaagaaaag ttctatttag ttagatttta agcgtaatat
    20341 gagattatgt atagagaaag gatgtatttt tagatgggaa aaatattagt gctagcagaa
    20401 aaaccttctg ttggaagaga tatagcaaaa gttttagggt caaaaaatga aaaaaatggt
    20461 tatatagagg ggccaaagta tgtgataact tgggcactag gtcatttggt tacacttgct
    20521 gaccccgaaa gttatggaga aagatataaa tcatggagct tagaagacct gccaatactt
    20581 cccaagcacc ttaagactgt tgtaataaaa aagagtggaa agcagtttaa tacagttaaa
    20641 tcgcaaatga atagaaatga tatagatgaa atagttatag caacagatgc aggtcgtgaa
    20701 ggagaacttg tagcaaggtg gataatagaa aaatcacagg taaaaaaacc tataaaacgt
    20761 ctttggattt cttcaagtac agacaaagca ataaaagacg gatttgcaaa attaaaaagt
    20821 ggaaaagaat atgaaaattt gtattactca gcaatagcac gagcagaagc tgattggatt
    20881 attggaataa atgcaaccag agctttgaca acaaagtaca atgcacagct ttcttgtgga
    20941 agagtacaaa ctcctactct tgcaatgttg cttaaaagag aagaagaaat aagaaatttc
    21001 aaacctaaag agtattatgg attagaatta attgctacaa agggaaattc agatataaaa
    21061 tttatttgga atgataaaaa caataattct tcaactttta gtaaagaaaa gatagaatcc
    21121 acattaaaaa aagtaaaagg tgtagacata aaaatatctg aagtaaacaa gtcatataag
    21181 aaaaaatatt cacctgcact ctatgattta acagaattac aaagagatgc aaataaaatg
    21241 tttggatttt cagcaaaaga aactttatct ataatgcaaa aactgtatga acatcataaa
    21301 gtacttactt atcctagaac tgattctaga tatttaacat cagatattgt tggaacatta
    21361 aaagatagaa taaaagctgt aaatattagt gattattcaa agatttgtaa tagattatta
    21421 aaaagcaata ttatagctaa taaatcgttt gttgacaatt caaaggtaac agaccatcat
    21481 gcaataatac caacagaaca gaaggtattc atgagtgatt ttagtgataa agagcgaaaa
    21541 atatataatc ttgtagtaaa aagattttta gcagtactat ctcctccttt tgaatatgag
    21601 cagacaacta taagtgcaac tgtagaagga gaaatattta aggctaaggg aaacaaagta
    21661 ataaatttag gttggaaaga aagttataaa gatattgaag atgaagatga gtatgaaagt
    21721 ataccaaatg ttttagaaaa agatgtactt aaggtcaagg gattaaagat aacttcagga
    21781 aaaacaaatc caccaccata cttaaatgag ggaacattat tgactgctat ggaaaagaat
    21841 ggtctaggga cagttgctac aagagcagat ataatagaaa aattattcaa ttcattttta
    21901 atggaaaaga aaaataaaga tatatatata acatcaaaag gaagacaatt attagattta
    21961 gttccagtag acttaaagac accagaactt acagcttcat gggaaaagaa attattggat
    22021 atttcaaagg gaaaacttag taaaaataca ttcataaatg acatgaaaga ttattcaaaa
    22081 gatattgtac tagaagtaaa aaatagtgat aataaattta aacatgataa cttaactaaa
    22141 aatagatgtc cagaatgtgg aaaatttatg ttagaggtaa atggtaaaaa aggtaagatg
    22201 ttgatttgtg aagatagaga atgtaatact agaaaaacta tatcacaagt aaccaattca
    22261 agatgtccta actgtcataa aaaattagaa cttcgtggag aaggtgaggg gagaatcttc
    22321 acttgtagtt gtggatacag agaaaagttg tcttctttca acaagagaaa gcaagaagaa
    22381 aaacagaagg catcaaaaaa agatgttaat caatatttaa aaaatcaaaa taaaaatcaa
    22441 gagacattta ataatccatt tgcagaagca ttagctaagc taaaaaacaa ataattttaa
    22501 agttattatt atacacaatt ataactttaa ctgtatatta gttatatgta ctgatataat
    22561 atagtgtata gtttaggcat gaaagtaggg ctgttaaatg gatttaagtg gcatatttaa
    22621 atactattgt aaagagtgtg aaaatacatg gaataattcg agtgttgaat tatttgagaa
    22681 tatagaaacg tatagtaaag attcacaaaa aaagagggaa aaagaattag ataaattgct
    22741 aaatacaata tcagttcatt tagagaggta tccaagtgat gctgtattga gaaaaatgtg
    22801 ggtaaaaaag ggcgaggttt tcttacaaaa gacattggaa aaagaaaata tttttaagtt
    22861 agaaaaaatg gatgtagagg atagaaaaaa atttttagat ataacaaaac agtttattag
    22921 agatgctaga aaatttgatg atgatttacc tataggtgat attatgcaag ctatgaggaa
    22981 tgtatggatt tcaaatgcat tacaattatt atttggtaaa gaagtatatt attcaaaagc
    23041 taactttgca tatagtatgt tatatccata tactgataat tatttagaca atacaaatat
    23101 agataaaaat gataagattt tatttaataa ctggttagaa aaaaggctcc tgggagaaca
    23161 cattaaatct aaggattatc atgaaagtaa agtatctcaa atgatagatt atattgaaag
    23221 tgtataccct agagaaaagt ttacagaagt ttatgaatcg ttattattaa tatttaaaag
    23281 tcaagtaaat agtttaaaac aacatggtaa ggaaaatcat ttgtgtaaag aagatttatt
    23341 atccatttct attgaaaaag gaggttcatc cgttttagta gatggatatt taataagtgg
    23401 attgatgaca aaggaagaaa tagagttttg tataggatat ggatttttat tacaaatatc
    23461 tgatgattta caagatataa aagaagattt aaaatacaac cataaaacta ttattacaga
    23521 gatgtcaaaa gagggtactt tagataaagt tgtaaataaa ctaataaatt ttactattga
    23581 gttaatagat agttttaaaa ttaataataa aaataaatct gtaataacta tgataaagaa
    23641 tgattgctta atgttaattt tattttctgt agtttataat gctgaatttt tttctgtagg
    23701 atatataaaa gaagtagaga aatttattcc atatacaata gattattcat tagagattga
    23761 agaaaaaata aaagaaaaat ttaaaaatat agatgtttta aataatgaaa atgaatataa
    23821 agaaatgatt gatattattt gtgcagagta gaaaaaaagg gagtgtctta gaatagactt
    23881 tttagtttat gaggcactct ttttgtataa aatcaaatgt attttcatca ttttaataat
    23941 taaaaagagc ttatccctat ttgagaaagc cctttttaat ttaaataaaa atgtcaaggt
    24001 taaactattt aaatgcagta ataatcccat tatcatgtaa gttttttatg attgtagctg
    24061 cgatagggaa tccaaataat ccagctattc caaatagttg agctccaaca aacatacaca
    24121 ttaatgttat taaaggatat aggccaattt ggtgtcctac tacctttggt tctaatattt
    24181 gtctaacaac ggttataatt atgtatagta ttaaaagtcc tattgctaaa ttaatatttc
    24241 cattaatgag tgagataatc atccaaggtg tcaaaatact tcctgtaccc aagactggca
    24301 aaatatcaac aagtgctatg atgacagata tagtaaatgc attttcaacc tttaggatag
    24361 taagtccaat aagtagttct atgaatgtca ctgatagtag tattgcatag gcttttagta
    24421 gttttaaaag agcattaata ctgctatttt ttatatttat tatcataaat tgactttttt
    24481 ctggaaattg ttttaagata aagtttacta ttttttgata gtcaaaagta aagaaaaaag
    24541 aagcaattaa agtaataata agttttaaca taaatgcagg tgttttactt gcaatactag
    24601 caatagcatt gagtgcattt gttgaaatag aagctactaa agacatcata gattgactta
    24661 tattatccca accaagtatt aatgctaggt taacattagg tgtactagca tctatctttg
    24721 aaaataaagt atttagcgtt ggttgtatac ttgatttata tatttcagga agtctaaaaa
    24781 atacatcact tacacttgca aatatcttca caccaaagct aactaataaa aatatacaca
    24841 acccataaaa aattaaaagt attactatag aaataagtgt acgatttaaa tttgtattat
    24901 ttgtaataag tcttataata ggtcttaaga tggatgcaat gataaaactg attacaaaag
    24961 gcattaataa tggcaataag aattttaata caatatatac aatacttgtt attaaaacta
    25021 catatgtaaa tttaattata aaattctttt gcttttgaat atccataata taaaacctcc
    25081 aatgtatttg atattattct aaatatggtc t
//
LOCUS       DAENCV010000050        24891 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAENCV010000050 DAENCV010000000
VERSION     DAENCV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.050"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(350..700)
                     /locus_tag="KQD82_002060"
     CDS             complement(350..700)
                     /locus_tag="KQD82_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG4380530.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(716..1132)
                     /locus_tag="KQD82_002061"
     CDS             complement(716..1132)
                     /locus_tag="KQD82_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG4380531.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(1151..1342)
                     /locus_tag="KQD82_002062"
     CDS             complement(1151..1342)
                     /locus_tag="KQD82_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG4380532.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(1493..2308)
                     /locus_tag="KQD82_002063"
     CDS             complement(1493..2308)
                     /locus_tag="KQD82_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG4380533.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(2355..3293)
                     /locus_tag="KQD82_002064"
     CDS             complement(2355..3293)
                     /locus_tag="KQD82_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4380534.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGRIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVMISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(3290..4162)
                     /locus_tag="KQD82_002065"
     CDS             complement(3290..4162)
                     /locus_tag="KQD82_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4380535.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(4187..6115)
                     /locus_tag="KQD82_002066"
     CDS             complement(4187..6115)
                     /locus_tag="KQD82_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG4380536.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(6227..6604)
                     /gene="gcvH"
                     /locus_tag="KQD82_002067"
     CDS             complement(6227..6604)
                     /gene="gcvH"
                     /locus_tag="KQD82_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG4380537.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(6691..8817)
                     /gene="cdhC"
                     /locus_tag="KQD82_002068"
     CDS             complement(6691..8817)
                     /gene="cdhC"
                     /locus_tag="KQD82_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG4380538.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(8874..9680)
                     /locus_tag="KQD82_002069"
     CDS             complement(8874..9680)
                     /locus_tag="KQD82_002069"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG4380539.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(9730..11097)
                     /locus_tag="KQD82_002070"
     CDS             complement(9730..11097)
                     /locus_tag="KQD82_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG4380540.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASEAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(11128..12072)
                     /locus_tag="KQD82_002071"
     CDS             complement(11128..12072)
                     /locus_tag="KQD82_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG4380541.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(12092..12862)
                     /locus_tag="KQD82_002072"
     CDS             complement(12092..12862)
                     /locus_tag="KQD82_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4380542.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(12918..14303)
                     /gene="lpdA"
                     /locus_tag="KQD82_002073"
     CDS             complement(12918..14303)
                     /gene="lpdA"
                     /locus_tag="KQD82_002073"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG4380543.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(14347..15228)
                     /locus_tag="KQD82_002074"
     CDS             complement(14347..15228)
                     /locus_tag="KQD82_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG4380544.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(15264..15938)
                     /locus_tag="KQD82_002075"
     CDS             complement(15264..15938)
                     /locus_tag="KQD82_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG4380545.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(15975..16847)
                     /locus_tag="KQD82_002076"
     CDS             complement(15975..16847)
                     /locus_tag="KQD82_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG4380546.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(16939..17571)
                     /locus_tag="KQD82_002077"
     CDS             complement(16939..17571)
                     /locus_tag="KQD82_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG4380547.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(17676..19352)
                     /locus_tag="KQD82_002078"
     CDS             complement(17676..19352)
                     /locus_tag="KQD82_002078"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG4380548.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(19601..20380)
                     /locus_tag="KQD82_002079"
     CDS             complement(19601..20380)
                     /locus_tag="KQD82_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4380549.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASLGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(20690..22609)
                     /gene="cooS"
                     /locus_tag="KQD82_002080"
     CDS             complement(20690..22609)
                     /gene="cooS"
                     /locus_tag="KQD82_002080"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG4380550.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(23216..24127)
                     /locus_tag="KQD82_002081"
     CDS             complement(23216..24127)
                     /locus_tag="KQD82_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG4380551.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(24243..24821)
                     /locus_tag="KQD82_002082"
     CDS             complement(24243..24821)
                     /locus_tag="KQD82_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG4380552.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT         7238 a         5150 c         2865 g         9638 t
ORIGIN      
        1 tatctttaaa tagtaatact caatatataa ttacttaaca taaaataact caaattgctt
       61 gatgaggctt ttgcagtatt taaagaatat ataattattt aacattaaat aatccaatct
      121 tgtaataaaa tttactcatt aaagtatatt acagaaaata cctcaaaata aactttttag
      181 ttcatgagat actctttttt acatgaaatt agatatattt ttatcatttc aacaatgaaa
      241 gggaggctta tatccatctt tagacaaccc cttagataaa atttttaata attaaacatc
      301 acaaaatata aaaatcgctt atatcaaacg tataagcgat taattatatt tacatatgtt
      361 ttttagcctc taacaacatt ttattagctt cttccatata ctctatagcc ttttcaatac
      421 tctttgctgt ttcttctttt ccaatagctc tagccttttc aacccactct ctaaatcctt
      481 cttcatgagt ttcattgtga tttatccaat gtactaaaag tatttttaaa gttttttcat
      541 ccttagactc tgatgattcc tcatgactat gttcgtggtc gtggtcgtga gcatggtcat
      601 gtggatgttc atgacatata tctccatgag tatgttcgtg gcagtggtca tggtcatgat
      661 gtccatgatg atatcttaca atctctttat caaaaatcat tagttattcc ccctgttatt
      721 ttatattaaa ctcttttttt ataaggttta cagaatcttt tacaagtagt gctaatggtt
      781 taccttctaa tccaactaac tccacatttt ctggcataat aggtaaaagt atcttaagtg
      841 cctcactgtc tgcaatagct tcacttatat tacaagtaac ttctcccatc atagaattag
      901 gtattattac agaaataggt gcaactatta tatttgcttt tttagctgat actactatag
      961 cattttcccc tgtagctcct ttattagcat gagctttcat catagaacta gttgctatag
     1021 aattagtccc tagtgcataa atttctacat atgtaggtaa ctcttctctc aaagaagaaa
     1081 ctatctgggc tccaatacct ccacccattc catctatcac tgctattatc atatgaaatt
     1141 cccctattaa ttaattttct tgtattatta tcttatggtc catcaatctt atttctttaa
     1201 ttatcccatc tattatcttt tgctctccta ataaatccgt taaataaatc tttccatcac
     1261 aagggtctat attaaccaca ttttccataa ccttttctaa ctcattatta cttttacaaa
     1321 tgaaagctga tgattcacac atataaatct cctcctaata tgatttttat taaaaatcaa
     1381 aaaaagattc ccttaggtat ttcaataaac caaagaaacc ttcatgattt cagtttttgt
     1441 atattattat accttaagta tatcacttta atttatgtaa agtcaattaa agttaacgct
     1501 ttttattata ctcttcattc ttgtattttt catatagttc tttaactaat tgcttctcat
     1561 aatcagtcaa atctccctcc acaaactcaa cattaaattg ttcagctaat ccctttacta
     1621 tgtttttctg tagaatatct atgtctattt ttctgccaat ttcattttct attccacttg
     1681 cttttttagc tgtaaatttc atagctcttt ctttcaattc tggcgtctta gtttttatta
     1741 atttaaacaa tttttctaca tcaaaatcca gtattattga accatgttgt aataaaacac
     1801 catcttttct gctttgagca cttccaatta ccttcttatt attaatagta acttcatatg
     1861 atgcatgtgc attaaaacat gcagctgata aattttccct acttattctc tcgcctctat
     1921 ttaaattatc tgtttctata ccacttagat tcaatccttt aactagacct tcacttatat
     1981 atctataaga taggttgatg ctcttatcga ttaaaggatt atcttctcct ataattatac
     2041 tataggttag ctcattatca tgtaatacag ctcttccacc tgtaattctt ctagtatagt
     2101 ctatattcat acatctacac ttatctaaat ctatctcatc ttccaatttc tgaaaatatc
     2161 ctatactcaa acaagctggt tcccatgtat aaaatctaag agttggctta ttatgtcctt
     2221 tcttatatgc agtaaatata gcttcgtcaa ttgccatatt cattgctcct tcatatgatt
     2281 tattatgtat aactctccac tgattcatga ttattacgcc cccattttta gtataaatat
     2341 atcctagaaa tcctctatag atttatttct cttattgcta aatctatctc actttgattt
     2401 aaaggtcttg aatagttgta tatagtactt cctggtcttt cattatagta tggtctatta
     2461 caatccttac aacctcttat ttcaaaagga tagcctttgt ttatgtcttt taaaatatca
     2521 ttgtcaagct ttatactatt taagtatcca tttttaaact taaaatactc ttttggataa
     2581 cctttattta tcatataaga cattaactgt acccttctat aactttctat gcttggttga
     2641 cttatctttt ccattttagt tcctctaact ggagtaaatg caaataaact aatcattaca
     2701 tcattttcct ttaaataagt atataaattg tatatatctt catgactttc acccatacca
     2761 actattatat gtgtacttat tttattggga tatgattttg acatttcagt tataaatttt
     2821 aacttttcat catagttatt tccttttatt ttttcatata gttctttatt tgctgcatct
     2881 attgctattc ctattttatc aactcctact gaaaaaaact tctttatctg ttcatcattt
     2941 tctaattttg cagatagtga aattggcatg tctatcctac cacttatata gtttataaag
     3001 tctaaaacag agttatgtgc ttcttcactt gccattgatt gaatacatat tctctttata
     3061 ttttttcctt tataagcttt taaagcatct aatatttctt cttttgaaaa ctctggccaa
     3121 attactcttg ataatttatc ttttctagta ctactttcta ttgattgaga acaaaaactg
     3181 catttattta tgcatttttc tccaatcatt atatatgctg tggtaggagg tatatcgctt
     3241 tttttgttta atatcccaag ttctattgct gtcccaactg atagtctaat cataaaacgc
     3301 aacactcctt acactcattt attgtcatat tcatttctat agccttattc tttgcagacc
     3361 ttgatggtaa tactattcta ttgactccac acattataga taattggtct agctcttttc
     3421 tatacactcc tcttggtctc atacaaccta gattaatttc agtatctggt aaattaattc
     3481 tagcttcaca taaaatatct gctacccctt ctaactcagg aggttctaca ttttcatact
     3541 cagttccttt tgttggaata aggactataa atgtaagctt attaggagga tttttttgta
     3601 ggtattctat aatttcatat tcacccttta tttgtcctcc ttttaaacca atacatatat
     3661 gtggtgctac ttctgtatgt tcttgtattg tattatagac ttcaatataa tcttctttac
     3721 tctttttcat cttataaact tctcttattg tctcatcatc aaacactaaa tcaaaagaaa
     3781 ctatatctaa atacttacac aactctacaa tgctatcttt atccataaga cctaaatgag
     3841 cattcaatct atatccttgc tcttttaagt ttttaatggt attaatatga gtatttatag
     3901 ggacatctcc ctcataacta caaccgccac tcaatagaaa gctagttata ttccttgatt
     3961 gtactttttc ctcatattct tctattggca ccatattgtt taagtagtga ccgttgcaat
     4021 gggcacattt caaactacat tgattgcttg tagtactaag agctagtgtc tggttaggat
     4081 aagcaaactc tattttattt ccaaaattct ttaatttaat atcaaatgct attttcattt
     4141 tttcagatag attcttattc atagtttatt ccttcttaag tacattttac tcttctagta
     4201 atttctttgc tgttgggaac tgctctatat tcgtatgtgg taagaaacat gcagaaataa
     4261 attcatccat ataacttgga tatacactaa gttcaacata agttatttga ttacctattt
     4321 cttcaagttt attttttgca tctttactta taagagctaa gtaagaacca acaagtgaac
     4381 tattccctat ataagtaaat ttttctctat cgatatcagg aagtaaccct ataattattg
     4441 agttttctat gtctaggttg tttcctatac cacctgctat ataaacccta tctaatatac
     4501 taaaatccat acctaaacta tctacaagag tgtatgcgcc tgaatatata gctcctttag
     4561 ctctgatgaa attatctata tcaacttcat tgactactat atcattttct aaatcaaatt
     4621 cttctttaaa tgctaataca tactctccta tctcatgctc attaaatcta actctcttat
     4681 tatctaaatc cctgtatatt ttacctcttc tatctattac acctttagta atcatttgac
     4741 agattaaatc aattatacca gaaccacata taccaactgg tgcacattca tcaataatct
     4801 ttaatgttgg ctctaaagta tctttatcaa taattacttt ctctattgct cctgctgatg
     4861 ctctcattcc acagcttatc cctccacctt caaaagcagg tcctgctgaa catgcacagc
     4921 tcatcatata atctttattt ccaaatacaa tctctccatt agttcctaaa tctataaata
     4981 aaacattttc ttcacttgac catatccctg ctgataatac tcctgctgtt atatctcctc
     5041 ctacataact tgcaacagat ggagctaagt acacatatgc actgtcattt acaaaaagac
     5101 caacattttc acccataagt tttggagatt ttaaaaatgg tggtatgtaa ggttcttgtc
     5161 ttaagaagtc tgtatacaca cccaagaata aacttgtcat agttgtattc ccggcagcca
     5221 ctaatgtaac tacatcttcc ttatctattc catttctttc atatattgtt tttaatagtg
     5281 gatttatagt ttcctcaact atagctttat gaagtgtctc taatccattt ttcttagttg
     5341 aatatataat tctatttata acatctgctc catattttat ctgtgcattt ccagaagatg
     5401 ctttatctac tacctctttt gagtataggt ctactaaaca aacaactact gaagttgtac
     5461 caatatctat tgcaactccg tataaggaat tttctttatt tccttgttct atatttatga
     5521 ttgtaagttt cttttgtttt tgaacatatg ttattgtaac cttaaaatca ctctttctaa
     5581 atacagttgg catttttcta agtatatcca atctaaaatc tatttcatta tatcctaggt
     5641 tatttctaac atatctttcc aatctatcta catcacttat attatcatct aaatttggtt
     5701 cttccatttc tatgtatttc ttttttatat ttgttttaaa ttgaaggtta tgttcttcaa
     5761 ttattttttt agctctttca aaaatttccc tatcctcttt tttattacta ccttctatct
     5821 tcataccatg catagaagat gatactttag aaggaacttc aatttctata tctgatatta
     5881 cctttgtaca acaagctagt atataacctt gctcccactc atcatctgtt atatgtcttg
     5941 ttttttctgt atcaactttt ccattcaata attttacttt acacttccca caagacacgt
     6001 ttccattaca aggagcatct atgaatatat ctgcatttct tgcaacctct agtaatatat
     6061 ccccctcatt acaataaacc tctttattgt ttggtgtaaa acttactttt atcatgttat
     6121 ccctcccaat ttgttctata tatgataaaa ggaaagtata gctaaatttg cgtgcactca
     6181 aattttacta tactttcccc aattataata atatcttaat ctttaattat tctaatccag
     6241 cctcgtattc tttatcactt aataacgtat caagttcagc cacatcagct agttttactt
     6301 ttaatatcca gttttcatat gggtcttcat ttatacattc tggctcatct tctaattttt
     6361 cattaacttc caatacctca ccattaacag gagatattaa atcagaagct actttagaag
     6421 actcaactac tccaaaatct actcccttag ttacagtatc ttctacttct ggtgtttcaa
     6481 caaacaatat ttctcctaat tgatcttgag catagtctgt tatacctata tatacaacat
     6541 ctccatcaat tacttttacc cactcatgat ctttagagta ttttaattct ggtaataatt
     6601 tcatttcgat agacccccaa tattatatta ttgattaaat tacttaaatc atattaatat
     6661 tatatcatat tattttaaat tttccatcaa ttacattaca ctttccatag ctaatgctgg
     6721 atgacctttt tcttctaaga aagctaatac tccttcagaa tcgtcagcta tagtttcatc
     6781 acaaaccata tcacagaagt tttctattcc agtcatttct ttaactgtag catttaattt
     6841 atcagctaca taatctttta attcttttgg catccaaact attctttctg gtccaccttc
     6901 tgcataagcg aattttttag aagatatgaa atgtcttccg tgtcccataa atcctggagt
     6961 ttgaactcct ccacctgtca tagaagcaag ttctccgaaa gtcataccaa ctggagtaac
     7021 acttgcaaat tctctgttaa ctacaacaaa tccatttgct tctggcatta taccacagat
     7081 acattcgaag catccacaag aagtcattgg gtcttctagt atagagtata atgtaacaga
     7141 ttcaactgca ccttgagata tttggtttac agtttcattt actgaattcc atacacctgt
     7201 tctatcatct aagcactcgc ctttttctat tggttgacaa ggacctgttg ggtctagttc
     7261 tttagtagcc ttagcatcta accagctaac tgctccacaa agaccaagtc tttcaggagt
     7321 aacaacacaa acgtgagctg gtgcaaatga ttgacataag ttacaagaat agaaagtgtc
     7381 aacactttca tcaactaatg aagctaatct ttcatctcta gcattgtact tagctattgc
     7441 ttcacctttt aattcagaaa ctttttcagc atctgtaatt atagttattt cacacttatc
     7501 aactactgat tcaaattcat ctaacatctt agcatataaa acttctccta tatgctcaag
     7561 tctaaatcct ttatcaaatg catctttaga gattcttacc caagccatat ctctttgtcc
     7621 aacgtgcatt acaccttcta tatagtttaa gaagtagtgg aaacgtcttt caagaactgg
     7681 ctcgaaatct tcttgcatat ttttaccagc tattttaact attacagcaa gtggtaatct
     7741 taatacacca tctgcatcaa cttcatctat atttggtcct attatttcta tcttatggtc
     7801 ttcaacttct gttgcttctt tcttaacaac aagctcccaa gcttcagttc tgtttccacc
     7861 aaactcagca aacatatcgc tctttctaat tctctcacct tcaaatgctg ctgcaaatga
     7921 aactgggata ggtatttcag taacttttat ttttattcct ctagcttcta atgaagtagc
     7981 tactatctta tcgtaatctt tttgagttaa taggttcatt ggaacttcaa gaactgtttg
     8041 gtcagttata actgggaatc ctaaagctat tgctccagca cctgcagata caactaattc
     8101 acttaatggt ccaaatgcat taacaaatgc aggaactcta ttagctgtgt actctaataa
     8161 tccgtttaaa tcaccaggag ttaatccacc gaatattaat gctgctctta ctgcaacaga
     8221 tacaacgtgt ataacagaag ttacatcata tcctagaggt ataactctta gctcaagacc
     8281 catttttact ccagcttcta tagcttggtc tataacttta ccaactaaga aagttagtaa
     8341 acctttagat tggtaatctt ttataacttt tgctgctgat tctgcatctg gacactcacc
     8401 aagaacaact gcaacaccag gtatatctcc tgtaactaat ggtacaccaa gtgatctgat
     8461 tatagggtca gttatatgac ctgcacatgg ctcactatat ggagcatcca tagttgaata
     8521 ttttaatgct tcaattactt ctgctgctaa agctgtagct aaaccagcat taaaagcatg
     8581 ttctagtaaa tgagttctat ttattaaaga ttttactact tctaaagcac cttctaagtc
     8641 ccctaaagtg ttcatctttt gtcctgttgc agcatatata caaggtaatg aatatgctgt
     8701 atcagggaaa gcaactttat gctcttttcc attcttttct atagcttcag ctaacatagc
     8761 ttgagccgca cctaaagctt gttctgaccc tgtaaagatt atattatata gattcattct
     8821 atttccccct ttaatattta cacctatttt ttattaacta taccctaaaa accctagaaa
     8881 gcaaatccac cttcatttaa ctctaagaaa gagtctgcat ataaagatgc cccgttgatt
     8941 atgtcacaag attttacagt tttcattaat tctgggtcac atggattcat tatagctgaa
     9001 ctaaatccat tagccattgc cattgctaag aatgcactat ctaaaacagg tctaacatgc
     9061 ttaggacatc cattagatac gttagataat cctcctgtag ttaaaagtcc catctcagtc
     9121 atcattttta tagcttctaa aacttctact tgtttttctt gcataccttt tataactaag
     9181 cataatgggt caaatagtat atcagttggc tccattccta acattaagcc tttttctaat
     9241 atttcttggc agtaagccat acgctcatca ttatctcttg ggataccttc ttttgcacat
     9301 aaacctataa ccattgcttc gtattcagct gctatatcta ttaaatcaaa tcttgaacca
     9361 gcatcagcag agtttattat tggttttgca ttagttctat cataaacttt tagtccagct
     9421 tcgatagctt ttttattagc tgtatctaat gctataggaa cattgttgaa ctcagattga
     9481 agtaatttga ttccccatgt cattatttct tcgccatctc tttcggcagg tcctatatta
     9541 aaatctatat agtgtgctcc tgcttctaat tgctcttttg ctctttttaa gattggagct
     9601 gggtctcttt ctgctaatgc ttttcttatt gaaggtgata tacaatgtat tctctcacct
     9661 ataatcataa atttttccat ttataatgtc cccctttaaa tttaatatta atagttagtt
     9721 aatattttat tagtttgttt ggcatgcttt ttcttggtat ccttttaaga acttaggtaa
     9781 ttccatagat tcctctggtc ctataactac attccatcct ggtaagttat cttctatatc
     9841 acctttaagt actgcaactt ttcctggaat gataaggtct ttacaattag ttacttcttc
     9901 taccttactt tctttaatga aagcagagat tgagtttcca ccaaatttac cagcagccca
     9961 agatgtaaga actgaatatc cacctgcatc tggtattact aaccatactg gaacttttga
    10021 tctttctata tccccagcaa ctatgaagta agttaatgca aagtcaacag taactaatac
    10081 tggagaattt tcatctgggt tgttgattgg ataaatcttt ggttcaactc tcattggtct
    10141 ttgtgggtca gtgtatatgt tttgtcttag tgcaaataat ggtaatgcct tagcataact
    10201 aatatcatct attactatta tagaaccgta ttttatagtg aatattgatg ataaagcaac
    10261 ttccatcata gggtttgaat tagataatct atttgcaaat actattgaag gatatccaaa
    10321 tgttctatct tgctctttta aagctgttct tctaacttgt atagcattag tgtaagtatc
    10381 ttttatgttt tctcctgtta catctaatac taattcttta tatccagcgg cttgaattaa
    10441 ttcaactgtt tcatataatt cttctaagct accagctttt acacctaatg gtaaacttgc
    10501 tcctttaact acttctatca tatctttgta gttctcttta gttgctccat ataccattgg
    10561 tctagcatct tttaatactt caacagcttc tttaactact tgtgcatcat cacaagctaa
    10621 aatataagct acttctaatc cactgccttt tattttatct attaatttta aatacgcatc
    10681 tttatctcca caatactcta aaacagccat ttcaactttc atttgttcac caattcttac
    10741 atagtcaact tttttcatgt tagctatttt agcatctact gcttcatcac tcatacaagt
    10801 acagaatgaa actgcatatc tatttctact tactaatgtt ttttcatgtc taaataatac
    10861 tgtttctcca cctagttcat actcagatgc accagcacca acttttaaag ctttcattaa
    10921 aggtgcagta gcctctgata attttgctat agcgtcatca gacatatgtg ggcatttacc
    10981 tacttctaca gcttctgaag caaccttcat agagaaagcc atacaagttg ggaatccaca
    11041 atccttacag tttttctttg gtgttaattt aaatatatct aaagctttta atgccattat
    11101 tttttccccc taaccttaac aatttaatta agctaatgcg ttaaccaatt cttttatagt
    11161 ttctattgat tttggatgac gaagtataac tgcgtttgaa ccacctacta aaacacttgc
    11221 tgcagttgaa acttccattg ctatagttct ttcttctgga caaccccaat ctggctcatc
    11281 ttctattgga gctatagctt ctttaacatg acctacttca aaagctactg gtgttatgat
    11341 aggcatttgt aatgtcttat cattttgacc aaatgctgca agtctaattc tatccatagt
    11401 agatgcaacg tactcatatc cataaccaac tgctgaacat ccaacattca taactatatt
    11461 ttcaccttta acgcctagtt gagttaacaa tacgtttaat tgtttagcta ggtttatatc
    11521 aacagaagat tcagctccta ctttatgtgc ataagccata ccagctgatg ctcctactcc
    11581 tttgtagtta tcttctactg ctgacataaa taagcagtta tgtccatcca atgtttgagc
    11641 taatttttca aataatttac catctttttc atgatttcct gaaccagcta caactaaagg
    11701 taattttatt gcttcaatta ctgccttagc tacatcagca cattcatcaa ctgacttatc
    11761 taatccattt gggtcagaac catcaaattt taaacaaata aaatctgctt gtgtattagc
    11821 ttcaacatat ttagcccact ctactggaca attagctaca tctttgtata attccttata
    11881 tgaatcagtc cagctttcag gataaacgtc tgatatttgt atccctattt ttggagaatt
    11941 acctacttct ccatcaaaac tataaaaagg taatacattt tccccaccta atttaattgc
    12001 tttttcccct attcctactt caacttctga tatttttcca gaatattttt gagtagacat
    12061 tttaaatgcc atattttttt cccccttaag cctattttaa ttctaattta gcaaaaattt
    12121 ctttcatagc cactttagat tttgaatctt caggtaaatt aactaaagga atacctgtag
    12181 aatcatattc atatatcaat tcatccatag gtacaacacc gattagctct aggttatgct
    12241 tttcaatttc ttctttaaca ccatcattca taactccatt tggaacttta tttacaatca
    12301 aaagtattct tccaacactt aactttagtt cctcagctaa atctcttatt ctagcaacag
    12361 cttgtatgct acgtcttgag caatcactaa ccaataaaag tgtatcaaca tgtctagtga
    12421 cttttctact tagatgttcc ataccagctt cgttgtccat gactaagtat ttgtaatgac
    12481 cagatatttt gtttacttgt tctctaagta tcccatttac aaaacagtaa catccttcac
    12541 cttctgacct acccattact aataagtcat atccttcgcc ttcttcgatt atagaattta
    12601 atctaaattg aagatattgt gcctttgtca taccaccagg gaatgcattg cctaactttt
    12661 ctctttgatt tacttcttct cttatttctc ctattgttgc ttcaacttcg atacctaata
    12721 cttcatttat gttagcattg gcatcagcat ctacaactaa tactggtccc tttttgtcct
    12781 taaccaaata atcaatcaaa agccctgtga gacttgtttt accagttcca ccttttcctg
    12841 caacagctat attgtatccc attttaagac ttccccctta aatctagcca cagagcctta
    12901 tgtactctgt ctgtctatta taatttaggt acagaatgaa cacattctcc atgtacatca
    12961 tgaagtgctt ccattagccc ttcagataaa cttggatgtg gatgtattgc atcccctact
    13021 tgttctactg ttaatcctag atgaacagca agtgacaact ctgtcaataa atctgtagca
    13081 tgaggtccta ctactgctgc acctattatc ttatcagttt ctttgtcagc tataactttt
    13141 acaaatcctt ggaaatgtcc tatagcttgt gcttttccaa gacctctaaa atcaaattga
    13201 cctacattgt attctacacc ttcagcttca agttgctttt cagtcttacc aacaccagca
    13261 acttcaggtt cagtataaac acatcttgga attgctctat aatctactac cttagtctta
    13321 cctaaagcat tttcaactgc tacgatacct tctttagaag caacatgtgc taggaaagga
    13381 gtatctatta tatcgcctat tgcgtatata ccttctacat tagtttcaag atgttcatta
    13441 actactactt ttcctctttc catttcaatt cctatatctt ctactccaga attgtcaagg
    13501 ttaggtcttc taccaacaca tactaatgca tattgtgctt ctataacctt tccattagaa
    13561 agagttgcaa cagccttacc atcaactact tcacaagttt gtactccaat acctgtaata
    13621 accttaattt tatctttctt aaattgtcta agtagttgtt tagctacatc tttgtcttca
    13681 tttaatagta tttggtcaac catctcaact atagttactt ctgttcctaa ggctctaaag
    13741 aactgcccta tctcacatcc tataactcca ccacctacta ttaacataga ctcaggtatc
    13801 tcttcaagtc caagaacttc atcactagtt attacaactt ttccatcata tgggaacatt
    13861 cttggtacaa ctggaacaga accatttgct agtattatct tatctgcttt tatagtttct
    13921 actgttccat catctttagt tacttctata gtgtttttat caattaattt tccaaaacca
    13981 ttaactaagt ttactcctct tttttcaaat aagaactcta taccacttat taattggttt
    14041 acaactttat tttttctttc cattatggca gtgaagtttg gcttaactgt accatctatt
    14101 tctataccaa aatcctttgc ttcttttaca gtattcaata ctcctgaaga ggcaagaagt
    14161 gctttagttg gtatacatcc tgcatttaag caagttcctc caactcttct tttttcaaca
    14221 actgttacat ctgcaccaag catagaagct tttatagctg ctacatatcc tcctggtcca
    14281 ccacctacta ctactatctt catctttaat tcccccaaac ctaatataat ctacaatctt
    14341 gcaatactat aaacctgcgg catccagtat agcaggtata ttctcttctg ccccaattgc
    14401 cattatatga acaccatcac ataaatcttc ttcttttaat tgtttgataa gttcccctgc
    14461 catttttatt ccttcactta cccagttttc ttttccagct gctcttaatc tttcgatttg
    14521 ttcatctgga acaaatattc ctggtacatt tgcagtcata aatttagcca ttcctggtga
    14581 ttttaaaggt actattcctg ctaatacctt gcaatccata tcccttgtta attctctaaa
    14641 ctttctcatt gtatttatgt cataaagtgc ttgtgtttgg aaaaatttag cccctgcttt
    14701 tattttcttt ttcattttta ataattgaac ttctattgga gaatattctg gagttactga
    14761 tgctcctaga taaaaatctg gagaaccttt taacttatta ccagccatgt ccgtacctgc
    14821 cattaaagtc tcagcagttt gaagtatccc cacagaatct aaatcaaaca ctcccttagc
    14881 ttgtggatgg tctcctacgc tagtatggtc tcctgttagt gccaacagat tgtttatccc
    14941 aaatacacct gctgacaaca tttctccttg tatagctatt ctatttctat ctctccctgt
    15001 tatttgaata actggctcta atccagcatc ttttaataat ttacatgtag caagagaagt
    15061 tgctctcata actgccgatt gaaagtctgt tacatttgct gcatgaactc ttccaactaa
    15121 cggctttgca cattctatta aatgtgaaag gtcagtccct tttggaggag ccatttcagt
    15181 agtaactgca aattttccac tttctaatgt ttctcttaat aagctcatct ctaatccccc
    15241 ttttaacctt ctaaaaaaaa cttttatgaa tttgctgctg cttctttctt tttctttgca
    15301 cttaaacttc ttggattatt ttgcttagaa tagtcttttg gtggtcttat ttctgctaag
    15361 ttatctaatt gtcctatagc ttctagtctt tcatatatct ttatccaagc acaatcattc
    15421 tctgagttta cttcacactt accattttta gctccaccac aagccccatt cataagacct
    15481 ttagcacaca tagttactgg acatatccca cctgtccaac caagttcaca gtctccacaa
    15541 gccttacagg cttcttcata ttctccaact ctttgtactt cccctataaa caaagtattg
    15601 ttggctggat aaactggctt atcttttaaa ttcttaacta ttgtctgtgt accatctcca
    15661 catgctaaag ataaaactgc atcagcttcc ttagtctctt cttttagagc ctttaaatcc
    15721 tttttagact ttaaaaggtt acaagcaggg tcaagcatta catatcctaa gatttttttg
    15781 ccttcacctt caagagtttc cttcattttt aaaacttctt cttccccacc acttttacaa
    15841 gttgctgcac attgattaca accaactaaa accagcttgt caaaatcttt taagtaccct
    15901 agcacttctt ctaatggttt attttcagaa attatcatat ctacttcccc cctaaacctt
    15961 ccacatatat atatctattt attgttttgt aatttacaag ccttaactac atgttttgca
    16021 agtatagatg ttgtaactga accaaccccc gctggaactg gagttatcat tgatactttg
    16081 tctaaaactg catttgtatc aacatctcca cataaatttc cttcttcatc aacatttata
    16141 ccaacgtcta taactaccgc tccatcttta acaaaacttt catcaaccat cttagctcta
    16201 ccaatagctg ctattagcac gtctgcctct gctgctacac ctgatagatt tttagttttt
    16261 gaatgacata ttgtaacagt tgcgtgttca cttaataaaa gcattgatac tggctttcca
    16321 actaccattg atcttccgag aactgttacc ttacttcctt ttaaatcaac attatagtgt
    16381 tttaatatct caactactgc tgtaggtgta catggtggaa atccactttt atccccttcc
    16441 attaccttag cagagtttat tggactaaaa caatctacgt ctttttccgg tgcaataaca
    16501 tattttataa cttcttcatt taagtgtttt ggaagaggtc taaaacataa tataccatta
    16561 acatttttgt catcattaac tctctttaat acatcaatat attcctcttg tgttatatcc
    16621 tcagctaact ctaacacttc tgttgttata cctatgtttt gacatctctt tagtgctcct
    16681 ctttcataag ataaatcatc acttcttgcc ccaactctaa ctattgttag tttaggattt
    16741 ataccttctt taactagtaa atcgacttct tttattaatt cttcactaat tttatcagct
    16801 acaggttttc cttttataat ttgtccttta gttgacattc cttccataca ttcacaactc
    16861 ccctacaagc aaacttaaat taatttattc tatataccac atattaataa catagtatct
    16921 actttcgtca tttaaaaatt attttccaag agctttttca actttggcat aaacttcatc
    16981 acaaattttt acaccatctt caactaactt atctacttct gtttttacct tattaacata
    17041 ttctttatct tgtatagaat ttatatttat aactacattt aattgtccac caagtatagc
    17101 agctcttaaa cactgaactc ctacaccaac atcacttata gcaagtcttg aacctttatc
    17161 aactaaatct tcatgtaact taatagcttc atagcaaact cttactatgt ttaatggtac
    17221 ttcacaagca acttttaatg ccttttccat tgtttctgct tttattcttt tttcttcttc
    17281 tgtttcttta ggaagcccgt aagcttttga taatggtaag aaacattctg catcatcatc
    17341 tatcattttt aataaatctt tttctaactt tcctgcctta cttaatatct cttttacact
    17401 ttcttcatac tcagcatact tcttcttccc tattgtaagg ttacatacca tactacctaa
    17461 agccatacct attgcaccta ctagtgcagc agctccccca ccacctggta ctgcttcttt
    17521 ggaagatagt acttctacaa aatcaacaca agttttatct gctattttca tgataatcct
    17581 ccttgaaagg atgtaggaaa atatctcaaa ttgttcgaga tattttacga actattttaa
    17641 gatatctcct actctttatt tataaattaa tttccttaga ataatccagc tattactcca
    17701 ttttcatcaa catctatttt ttctgctgct ggaacttttg gtaatcctgg catcttcatt
    17761 atttctccag taagtgcaac tataaatcca gctcctgctg aaattgttgc ttgtcttaca
    17821 gttattctaa atcctgttgg tctacctaat ttagtttggt catcagttaa agaatattga
    17881 gtcttagcca tacatactgg aactttagta aatccaagtt tttctaagtt agctatttcc
    17941 ttatcagctt caggagtata atctacacca tctgctccat aaatcttagt agctatagca
    18001 tttatcttgt cttttataga taaatcatct tcatagcaga atctaaagtt attttcttct
    18061 tcatccaata atcttaaaac ttctttagct actgcaattc caccttctcc accttttgcc
    18121 catacttcag aaagagcaac attaacacct aattccttac acttgtcttc aacaagttta
    18181 agttcagctt cagtatcaag tgggaatctg ttgattgcaa caactgctgg taatccatat
    18241 acttgagtta tgttttcaac atgttttaat aagtttggaa gtcctttttc aagagcttct
    18301 aggttttcgt tatttaagtc tgctttagca actccaccat tgtattttaa tgctcttaca
    18361 gtagcaacta ttataacagc atctggtttt aagtttgcca ttctacattt tatatctaag
    18421 aatttttctg ctccaaggtc agcaccgaaa ccagcctcag ttattacata atctgcaaag
    18481 tgcatagcca ttcttgtagc tataacagag ttacatccat gagctatatt tgcgaatggt
    18541 ccaccgtgta caaatgatgg tgttccttca agagtttgaa ctaggtttgg ttttaaagca
    18601 tcttttaata atgcagccat agcaccatta gcttttaatt gtctagctgt tacaggttca
    18661 ccttcgtagc tgtatccaac tattatgttt cctaatcttt cttttaaatc agatatatca
    18721 cttgccaaac agaacgctgc cattatttca gaagcaacag ttatatcgaa tccatcttgt
    18781 ctaactgctc catcaccttt tttacccata ccatcaacga tatttctaag ttgtctatcg
    18841 ttcatgtcta cacatcttct ccaagttatt ttcttagggt ctattctaag tgcatttcct
    18901 tggtgaatat ggttatcaag catagcagct aaaagattgt tagcagctcc tatagcgtga
    18961 aaatctcctg tgaagtgtaa gtttatatct tccataggaa caacttgtgc atatccccca
    19021 ccagctgctc cacctttaac accaaatact ggtcctagag atggttctct taaagcaact
    19081 aatacatttt tgtctagttt agcaaatgca tcagcaacac ctatagtagt agtagttttt
    19141 ccttcacctg ctggagttgg gtttatagct gtagttagta ttaacttagc ttttttacca
    19201 gtctctcttt ttagtagatt gtagtcaact tttgctttgt atttaccata aagttctaca
    19261 tcatcttcac ctagtcctaa tttttttgca acctctctaa tatcttgagg cttagcttct
    19321 tgagctattt caatgtcaga tttaaatccc ataactgaat ctcctcccac aacctaaata
    19381 gatttaaaat ataatttttg taaaaattgt attaatatgt aaaaattata ttttataaga
    19441 taattataca tgtaaaatta cttaaaaact agctactaag ctattaaaat attcaaactt
    19501 aatagctagt tatttatcta attacttact tattacctat ctattgccta tgtctaacct
    19561 ttaaccaact taaaatccca aatcaaaatt tctatctatt ttatttatcc ttaagtgaca
    19621 ttaatttatc ttttattgct ttaatctgtt ctttcgttgt ttcactagaa tcatatggag
    19681 attgattggc tctatcagaa tcaataacat cttggttata ataaataaat cctaaagatt
    19741 ctccatcttc taagttttgg attataaatt cttcatcttc cttatttcta attttatttc
    19801 ctactacaaa tactttattt acacctatat catgtccaag tttcttaact tttctataag
    19861 tctgtagact tctctctcct ggttctacaa ctactataaa agcatctaca ccttgggctg
    19921 ttcctcttcc taagtgttca attcctgctt ccatatccat aacgactaca tctttatttt
    19981 gtagtattaa atgtgagcag agtctcttca aaagaacatg ctctggacat acacatccag
    20041 taccaccaga atctacggtt cccaatgtaa gtaatcttac tccattgtat tctttacaga
    20101 aattttcagg gatatcgtct actttaggat tcatcttaaa catttttcca aatgaaccta
    20161 aagaagcagc tgttctatct gaaactaatt ttttcatttc tgatataggt actattgatt
    20221 catatacttc ttttggaaaa cctagtgcta aagctaaatt agcatcaggg tcggcatcaa
    20281 cagcaacaac tctgtatccg tcttcagcaa acatcctaga gagcattgaa gaaaaagtcg
    20341 ttttacctac tccaccttta cctgttattg ctatcttcat aatccctacc accttttatt
    20401 actatttgct ttttagtagt taattttaca gttaaatatt ttttatactc tatattttta
    20461 tacactaaaa ataaactaca gctagtcctt acaggaacca attaaattta ccctactagc
    20521 ttttcaaagc agcatgtata gttttattat tctttaatat tgtttatatt ttatttattc
    20581 agtatattca ttatattttg atgttcttta ctttaatatt tgactttatt caactttact
    20641 caaccagaaa cttcttataa aaacgtttat tctataactt cactattttt taagcttcag
    20701 ccttttctgc cattttttct tcaactaatg cttcaaagtg aacacgtttt ttctctatat
    20761 cttctattat tgtagctgct aactcttttg gatttgtatt tacagtaaat gtagctccaa
    20821 ctttatcttt taattcacta gttaatatat ccactgctct tgaagaacca gctattggag
    20881 gcattattcc taaataagtg tctataccag aagctacaac atagcatcct atagcaactg
    20941 ctttttctga catccactct ggagctatac ctacaactgg aaggtcacac atatccatat
    21001 ctaagtaatt agctgctgct ccaaccaaat ctagtatacg acttatatca acacaagaac
    21061 ccatatgtag tactggtggg attcctacta atttacagac agttgcaaga cctttacctg
    21121 catatttttc agccgcatca gattccatta aaccaaactt agctgctgct gaagctccac
    21181 aacctgtagt aactactatt atgtcgtttt tgattaattc tttcattatt gttacgtgag
    21241 cctcatttga aactccttta gcattgttac atccaactac accagctgct cctcttaata
    21301 ctccagcttt tagacagtct gttaatggct taactgttcc tgctgggtct atatgagagt
    21361 ttacaactct gtctaattga tttactactg cttctacact atacccaact gttgcgcctg
    21421 attttaattc aggtataagt acttttgatt tatctctatt tttaaagttt aaaatagctt
    21481 ctttaactat tgctttagca tcatcaagtg cttgttcttc tctaaattca atataagttg
    21541 aacctgttat cttagctttt ggagaagttg ttacaaattt agtatggtaa caatctgcaa
    21601 ctctagctaa tgcagggaat atacattgaa cgtcaactat tacagcttca actgctccag
    21661 ttattattgc aagttcttgt tgatggaagt ctcctgctat ttttacacca tgtctcattg
    21721 taatctcatt acctgtacaa cacatacctg ctaagtttat tccatcagca ccaacttctt
    21781 tagctaaagc tactaagtct ggctcttctg atgctaaaac tatcatttct gataatgctg
    21841 gctcatgtcc atgtaatatt atgttaactt tattttcttc taatactgct aaattcgcct
    21901 ctgttcttct tggcactgga gttccaaata agatatcgct tagacgagta cctatcatag
    21961 aaccacccca accatcagcc atagaagttc taagactcat gtgtattaaa ctatctatat
    22021 caccagtaca tcctatatga gtagagtgca ttattgttgc aatttctcta tctatagctc
    22081 ttggctctat agcgtattct ttccatattt tttgtcttgg ttctggtgca ttttttataa
    22141 atcttaatgt cccaaaaggt tttccaaatt ccattaatgc aatttctgcc acttcatgtg
    22201 ctacatcata gatatctcta ccttctgtct ctacatccca ttctttagct aaagtaatta
    22261 acttagcttc gtctcttatt ttatagtttc catttggatc agctaatgct aatgtatgtg
    22321 ctatatctct agcatggtct gaatgggcag ctgtcccacc agcaaccatt cttgcaaagt
    22381 ttctacctac tatagtatgc tcatctgctc cacataaccc ccttggagtc ttagggctta
    22441 ttctacatgg tcccattcca catattctac agcaaacacc ttgaagacca aaaccacatt
    22501 gtgccttaaa gccttctttt ctatcataca ttgtttctac gccttcttct ctagctttag
    22561 ctatcatctt ctggctgttt aaatcaattg ttaacatttt ttcatccata ttcctatccc
    22621 ctttttttga tttttaaaat cccccaggtg actgacttca tgtcataaaa taataaaata
    22681 aatgctttct aagttgatta tatactaatt ttcagtgctt tgctttagat ttaaatattt
    22741 ttttaacaaa attagataat acataaaaat tttattttct acacttaatt taagttgatt
    22801 ttatcgcttg tttttggcta tttgttaata tctaaaccta cttttttgat acttcagttt
    22861 aattaaatta tgttttgatt tttttaaatc aaatttgaaa tttttctagt catttttata
    22921 ttattgcaga ttgtttgtgt atttattatc tttcactact gtttggactt attacagatt
    22981 tttactccct aaacttaatt agtaaaaatt tcttagaata gcattttttg gactaatttt
    23041 tttgaatttt tttatttttc ctactttaat attaattata gcttcaaatt gaacatttgt
    23101 atatattcat ttgaagctat aattattttt tatataaaac ttttttaata cagcattaat
    23161 ttagacattt tgactatttt ttctacttta attcatttat ttcaagcgat ttatcctatt
    23221 tcatattttt agctacaata gccccatatg caatagaata taatttagat tcatgtgaat
    23281 cagctcttga cactaaaact ataggagctt ttgcacccat aattatacct gctgatttag
    23341 agtctgccat atatgtaaag gctttcccta ttccattacc aacctctatt gttggaacca
    23401 atatgatatc tacatctcca gatacttcac ttttaaaccc ttttatctca ctagcttctt
    23461 ttgaaacagc taaatcaaaa gccagaggac cttctacaat tacattttca ccaaattctc
    23521 cttctttaca agcttttgct aacatatctg catctacagt tgcttgcatc ttaggattta
    23581 ctttctcttt agctgccaaa catgcaactt ttacagtttc cattcctaaa gcttttgcag
    23641 cttttataga attttttaat atctttgcct tttgttcata atcaggagct atattcatac
    23701 ctccatctgt agttataagt aatttatggt atttttctaa ctcatatacc ataacatgac
    23761 ttaataaact atcagttcta agaccatagt ctttatttaa cacttctttt aatatgacag
    23821 atgtatctaa taatcctttc attacaaagt cggcttcttt acttgatact aatttaactg
    23881 ctatttctgc acactctttg atatcatcct tatccactat tttgatacca cttaaatcaa
    23941 atcctatttc ttttgatatt tctcttattt tgccttcatt tccaaccaat ataggagtaa
    24001 ttatatttct ttctacagca tcctttattg ccattaaaac ttctttatca tgagctgctg
    24061 ctacagagag tacaattttc tctcctccct taagttgttc taaaatatca tctaattttt
    24121 ttatcaaaag taacccctcc cttatatata tttaactaaa taataccata tttgctatac
    24181 cttttctcaa tttatttttt atgttataaa gaatttatat aactaaaaga cagtttaata
    24241 ttttattctg ggatttcatc aataactctt aaaagctttg caggaacacc acctacaata
    24301 gtattttctg caacattttt tgttacaact gctccagcag cgataattgc attatctcca
    24361 atagtaactc ctggaactac agttacatta gcaccaatcc atacattttt tcctattgtg
    24421 attggagaag gataagttgt accacgtttc tctggtgcaa taccatggtt aagtgtagcc
    24481 aataccacat tatgcccaat ttgcgtatta tctccaatct caatcccccc ctggtcttga
    24541 aaatggcaac tgctatttat aaaaacattt tttcctattg tgatgttttt tccacaatct
    24601 gtataaaatg gtggaaacat aaaaaatgat tcatctacct tttttccaat taactcagaa
    24661 aataaactac ggacttcttc tggttcatga taagaagaat ttaacttact tgtgattttc
    24721 attgcctcat tactcaactt aaccataaat tcatgtattt ctgatttact agaaacaggt
    24781 tttctttgat tgatatgtgt taaaaattca ttgatattca ttttatacat cctttcaata
    24841 tgtgctttaa tttatttgta cattctaaat aatattttaa taaaaattaa t
//
LOCUS       DAENCV010000051        24283 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAENCV010000051 DAENCV010000000
VERSION     DAENCV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.051"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            109..300
                     /locus_tag="KQD82_002083"
     CDS             109..300
                     /locus_tag="KQD82_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG4380553.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KQD82_002084"
     CDS             455..1864
                     /locus_tag="KQD82_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4380554.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="KQD82_002085"
     CDS             1995..2684
                     /locus_tag="KQD82_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG4380555.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KQD82_002086"
     CDS             2687..3622
                     /locus_tag="KQD82_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG4380556.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KQD82_002087"
     CDS             3619..4512
                     /locus_tag="KQD82_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG4380557.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KQD82_002088"
     CDS             4590..5336
                     /locus_tag="KQD82_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG4380558.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAAYRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KQD82_002089"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KQD82_002089"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG4380559.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KQD82_002090"
     CDS             6193..6897
                     /locus_tag="KQD82_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG4380560.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KQD82_002091"
     CDS             complement(7085..7513)
                     /locus_tag="KQD82_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG4380561.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KQD82_002092"
     CDS             complement(7550..8980)
                     /locus_tag="KQD82_002092"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG4380562.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KQD82_002093"
     CDS             9112..9696
                     /locus_tag="KQD82_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG4380563.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KQD82_002094"
     CDS             9712..10233
                     /locus_tag="KQD82_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBG4380564.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KQD82_002095"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KQD82_002095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG4380565.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KQD82_002096"
     CDS             12596..13366
                     /locus_tag="KQD82_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG4380566.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELSKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="KQD82_002097"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="KQD82_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG4380567.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="KQD82_002098"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="KQD82_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG4380568.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15813
                     /locus_tag="KQD82_002099"
     CDS             14728..15813
                     /locus_tag="KQD82_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG4380569.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15833..16702
                     /locus_tag="KQD82_002100"
     CDS             15833..16702
                     /locus_tag="KQD82_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG4380570.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="KQD82_002101"
     CDS             16695..17561
                     /locus_tag="KQD82_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380571.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYIFHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="KQD82_002102"
     CDS             17602..18315
                     /locus_tag="KQD82_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4380572.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18316..19485
                     /locus_tag="KQD82_002103"
     CDS             18316..19485
                     /locus_tag="KQD82_002103"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG4380573.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPSNGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="KQD82_002104"
     CDS             19489..20325
                     /locus_tag="KQD82_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG4380574.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="KQD82_002105"
     CDS             20326..21321
                     /locus_tag="KQD82_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4380575.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="KQD82_002106"
     CDS             21339..23027
                     /locus_tag="KQD82_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG4380576.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="KQD82_002107"
     CDS             23039..24106
                     /locus_tag="KQD82_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4380577.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9693 a         2714 c         4336 g         7540 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa cagctcttga
     1081 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcaaacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgtt agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatatata caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatacaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa agcctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggt aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attaaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt taccataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag taatttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agctgcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagaacta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaagggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac tttcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttccca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct tttttttatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagata gtttgttttt atttatttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    13621 caactctaat attcacttaa cttaagttgt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggttga attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaaca
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttattta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttca tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcactta caatgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctagc ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tatagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagattatg aaaatagaat attgaatgta
    15121 gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt aactagaggt
    15361 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15481 gaagatgaag aaataataat aggggatatg ctggttactg aatgtgaaat agtttcaaaa
    15541 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    15601 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg actctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatt ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagacca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggagcttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aagattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagcca
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 tcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagcataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaagaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggttgta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaagatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcatatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 gggtatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgct agctcaccca ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa gagtacacta
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag aagttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta acagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aattttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa acataaagtt taaagatttt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcaaatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattac agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtt ggaggaaact
    22021 acaatgtact ggccactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22381 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22441 gtgcttatat gatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ccataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccaatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tacacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gcttttaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggtaaaaat
    23041 gaaatttaag aagctagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttaca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aagttaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aacgaatact ctaaaaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aat
//
LOCUS       DAENCV010000052        24093 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAENCV010000052 DAENCV010000000
VERSION     DAENCV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.052"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..231)
                     /locus_tag="KQD82_002108"
     CDS             complement(<1..231)
                     /locus_tag="KQD82_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4380578.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNY"
     gene            415..759
                     /locus_tag="KQD82_002109"
     CDS             415..759
                     /locus_tag="KQD82_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG4380579.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            790..2391
                     /locus_tag="KQD82_002110"
     CDS             790..2391
                     /locus_tag="KQD82_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG4380580.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     RKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2410..3510
                     /gene="dprA"
                     /locus_tag="KQD82_002111"
     CDS             2410..3510
                     /gene="dprA"
                     /locus_tag="KQD82_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG4380581.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3543..5630
                     /gene="topA"
                     /locus_tag="KQD82_002112"
     CDS             3543..5630
                     /gene="topA"
                     /locus_tag="KQD82_002112"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG4380582.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5795..6580
                     /gene="codY"
                     /locus_tag="KQD82_002113"
     CDS             5795..6580
                     /gene="codY"
                     /locus_tag="KQD82_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG4380583.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6687..7952
                     /locus_tag="KQD82_002114"
     CDS             6687..7952
                     /locus_tag="KQD82_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG4380584.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNGDF"
     gene            7983..8753
                     /locus_tag="KQD82_002115"
     CDS             7983..8753
                     /locus_tag="KQD82_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4380585.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNVKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8862..9308
                     /locus_tag="KQD82_002116"
     CDS             8862..9308
                     /locus_tag="KQD82_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG4380586.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9326..10519
                     /gene="nifS"
                     /locus_tag="KQD82_002117"
     CDS             9326..10519
                     /gene="nifS"
                     /locus_tag="KQD82_002117"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG4380587.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10573..11013
                     /gene="nifU"
                     /locus_tag="KQD82_002118"
     CDS             10573..11013
                     /gene="nifU"
                     /locus_tag="KQD82_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG4380588.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11302..12381
                     /gene="mnmA"
                     /locus_tag="KQD82_002119"
     CDS             11302..12381
                     /gene="mnmA"
                     /locus_tag="KQD82_002119"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG4380589.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12524..12762
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12830..15469
                     /gene="alaS"
                     /locus_tag="KQD82_002120"
     CDS             12830..15469
                     /gene="alaS"
                     /locus_tag="KQD82_002120"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG4380590.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15494..15751
                     /locus_tag="KQD82_002121"
     CDS             15494..15751
                     /locus_tag="KQD82_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG4380591.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15761..17932
                     /locus_tag="KQD82_002122"
     CDS             15761..17932
                     /locus_tag="KQD82_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380592.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18138..18563
                     /gene="ruvX"
                     /locus_tag="KQD82_002123"
     CDS             18138..18563
                     /gene="ruvX"
                     /locus_tag="KQD82_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG4380593.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18641..18928
                     /locus_tag="KQD82_002124"
     CDS             18641..18928
                     /locus_tag="KQD82_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG4380594.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19210..19668
                     /locus_tag="KQD82_002125"
     CDS             19210..19668
                     /locus_tag="KQD82_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG4380595.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19785..20522)
                     /locus_tag="KQD82_002126"
     CDS             complement(19785..20522)
                     /locus_tag="KQD82_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG4380596.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20739..22415
                     /locus_tag="KQD82_002127"
     CDS             20739..22415
                     /locus_tag="KQD82_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG4380597.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22621..22821
                     /locus_tag="KQD82_002128"
     CDS             22621..22821
                     /locus_tag="KQD82_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG4380598.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22875..24038
                     /locus_tag="KQD82_002129"
     CDS             22875..24038
                     /locus_tag="KQD82_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG4380599.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNTLIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
BASE COUNT         9877 a         2478 c         4457 g         7281 t
ORIGIN      
        1 ataattacaa agtataaaaa ttaatataag ttctaaaaag taagaaataa gtataaattt
       61 attctcattt gataaagaaa aaaatctaac ttgattattt attataatta gaaataatat
      121 tatcatagtt atgaccggat acaatgtatt ttcaacattt ttcaaatagc ttacaactaa
      181 tataaataaa gataaatatc gtataaaagc aactaatttt tctctgttca ttaaaggcac
      241 tcctccaaaa aaataaactt ttcctatgaa aactagaata atatcataaa agaataaagt
      301 caatagaagt atttattaat attgatggaa ataaaatcaa tccaaaaact tgaatagtaa
      361 aattgtatac tgaccataat ttaaaagtga aaatttaatg tattggagtg aataatgaat
      421 aataaagaaa aaggtgattt tggagaaaaa gtagcagtta attatttgct atcaaaagga
      481 gcaaagatac tagaaaaaaa ttacagatta aagatagggg aaatagatat aatagcaaaa
      541 atggaagatg aaattatatt tgtggaggtg aaatcaaggt ctaacataaa gtttggctac
      601 ccttgtgaat ctgtaagctt caaaaaaaga aaaaagataa ttggagttgc aagttattat
      661 attataaaaa ataacttaaa taacactccc ataagatttg atgttataga ggtatattta
      721 ttagaaaaaa gaataaatca cataatgaat gctttttaaa aaagataatg tttttggaaa
      781 ggattaaata tgttaagtat tattaattca agcaacttag taggaatcga cagcttttta
      841 gttaaggttg aagtagatgt aagcaatgga atacctagtt ttaatattgt tggtcttcca
      901 ggcaaagaaa ttaaagaagc aagagaaaga gtaaagtcag caattcttaa tagtggatat
      961 aaatttccaa gcacgagaat agttgtaaat ttatcaccag cagatataaa aaaagaagga
     1021 gcttttttag atttatctat aagtataggc cttttaagag agttaataaa aaaagatgaa
     1081 aattacataa gagaaagtat gtttatagga gaattatcat tagatgggaa aataagaaaa
     1141 gtaagaggta tattgcccat aattatgggt gcaaaaactc agaacataaa acgtattttt
     1201 ataccaattg agaacataaa agaaagtcta tttgtagatg aaattgatat aattcctatt
     1261 aaaagtttaa aagaatgtgt cgatttttta aacgaagaga taaaagtcga taaagtaagg
     1321 ataatgtctt ttttggatga taagtcaaga aaagagaacg aagaattaga aaaagataat
     1381 agttatatag attgcaaata tactaagatt aataatgaag aaagtaaata tgatgaagac
     1441 tttaaagatg taaaaggaaa ttattttgta aagaggagtg cagaaattgc tgctgctgga
     1501 aatcataata tgtttatgat tggacctcca ggctcaggaa aaactatgat tgcaaaaaga
     1561 gtaaggacaa ttttaccaga tataagtgta gaagaaatga tagaagtaag taaggtctat
     1621 agtatattgg gtatgataaa tgaaactaaa ggaataattg ataaaagacc ttttagagca
     1681 cctcatcata caactacaaa gcaatcttta attggaggag gaatggatgc aagacctgga
     1741 gaaattgctt tagcacatag aggtatctta tttttagatg agatagccga atttgataga
     1801 aaaatacttg agactttaag acaacctata gaagatggat atgtaaatat atccagagta
     1861 aagtacagtg caaaatatcc ttgtagagta ctgttagttg cagctatgaa tccatgtcca
     1921 tgtggttatt atatgtcaga gacagagtgt agatgtagaa gtaacgaaat tgatagatac
     1981 ataaataaaa tatcaggtcc attgttagat aggtttgata tttttgtaga agttaattct
     2041 ataaaatatt cagattttaa ttctttaaaa caagaagaaa gctctcaaaa aataaagaga
     2101 agagtagaaa atgctagaaa aatacagata aatagattta ggaaggataa tataaaaaat
     2161 aatagtgaaa ttaaagctta caatttattt aaatattgta aactggaaaa agaagcttca
     2221 aaaacggctg agatgatatt taataaatac aacctaagca gtagaagtta tacaaaactt
     2281 ttaaaaatgg caagaacaat agcagattta gaagaaagag atttgataaa ttcacagtgt
     2341 attatagaag ctttttcatt tagaaaggct tattatagtt attttaaata gttttttgag
     2401 gtggatttta tggaaaaaag agatacttat ttatggttaa aatcaattgg tggaataaca
     2461 acaaaaacaa ttgaaataat agaaaatgaa atagtaaata ttgaagatat atttgatttt
     2521 tctgaaaaag aaatatataa tttaaaaaat ataagcttga atattagaaa aaatatagta
     2581 aaatatagag gtcacgctta tttagaaaat ataaaagaac tactttacaa aaaagcgatt
     2641 aaatatattt gtaaatatga taaagaatat cctgaaaatt taaagaatat atacaacgct
     2701 ccaaagttat tattctacaa aggtgacata ggtttagtta ataataattt taatatagct
     2761 atagtaggtt caagaaaacc tacagcctat ggaattaatt gtgcaaaaac aataagttgt
     2821 caactatccc aatatggagt taatatagta agtggattag ctataggaat agatgcatac
     2881 tcgcacatag gttgtatgag tggaaaatct aaaactatag cagtacttgg ttcaggagta
     2941 gacaatcctc ttccaaaaca aaatttacat ttatcaaata aaatattaga aaatggggga
     3001 ttattattat cagagtataa tattaactcg acagtagccc cgtaccattt ttctaataga
     3061 aatagaatta tcagtggttt aagtgatgga gtagttgttg tagaagcagc aataaaaagt
     3121 ggtgcactaa tcacagtaga ttttgctctt gaacatggaa aaaatgtttt tgcaatacct
     3181 ggtaatataa attctcaaat gagtagaggt tgtcataaaa ttataaaaga aggtgcaaaa
     3241 ttaattgaaa atatagatga tattttaaat gaatataaca tatttaatat tattgataaa
     3301 aaaataaatc aaaaatatga taatataagt ctaaatgcca agtctaagca aataattgaa
     3361 gctataaaaa gagaaggaaa cttgcatata gatagtattt gtgattatac tggtatagaa
     3421 ataaagtatg taaattcaat aatcaatgaa ttagtactaa atgagttagt agtagaaatg
     3481 aataataaaa cgtacagttt aaatgtataa attaagatta ggtagtgggg gtgtatggtt
     3541 ttatggccaa aacattagtc atcgtagaat cgcctgcaaa agctaaaact atagaaaaat
     3601 ttttagggaa aagtcattac acagttaaag catcagttgg tcatgtcaga gatttaccta
     3661 aaagtaaatt aggtgtagat atagaaaata attttgaacc tcaatatata aatataagag
     3721 gaaaaggcga tgtaataaaa gaattaaaaa aagaagctaa aaaatctaaa caagtatatc
     3781 tagcaactga ccctgataga gaaggtgaag ctatatcatg gcatttagct catatactaa
     3841 atttagatga atctgaggat tgtagaatag agtttaatga gataactaag gatgcaatta
     3901 aaaaagcaat taaacatcct agaaatattg atataaattt ggttgatgca caacaggcaa
     3961 gaagagtgct agacagactt ttaggttatc aaataagtcc tatattatgg caaaaggtta
     4021 gaaaaggtct tagtgcaggt agagtgcaat ctgttacaac aaagttaatt tgtgacagag
     4081 aaaaagaaat aaaggcattt attccaaaag aatactggac aatagatgtt gaggctaaga
     4141 cagtaaaaga caaagatata actttgaaat tttatggtaa aaatgatgaa aagattgaat
     4201 tagaaaatga agaagctgta aatgaaatac taaaagaaat ggaaggtaaa gatttaaagg
     4261 ttgagaagat agaatctaaa tcaagacgta aatcagctcc aaaaccattt acaacaagta
     4321 tgcttcaaca agaaggagca aataagttat tttttactac taaaaaaaca atgatgatag
     4381 cacaagaatt atatgaaggt atcgacatag aaggtgaagg tactgttggt cttatatcat
     4441 atataagaac tgactctaaa agaatatctg atgaagcaaa agaaaaagct aagggctata
     4501 tcgtaaacaa tttaggagaa aactactata aaaacactgg tgataaaaaa gaaaaaaagg
     4561 atactaaaaa ggttcaagat gcccatgagg caataagacc aacctcagtt gatagaactc
     4621 ctgagagtat aaaagcctca ttgagcaaag accagtacaa gttatacaat ttaatttgga
     4681 gaagatttgt agctagtcaa atggaagatt cagtgtttga tatactaaat gtagaatgta
     4741 aaataggcga tattgtattt aaggcaactg gttcaaaaat gaaatttgat ggttatacca
     4801 aggtatataa tttttcagaa agagaagaca aaattttacc aagtatagaa aaaggagata
     4861 tattaaatat agaaaatata ttgccagacc aacattttac acaaccacca gcaagatata
     4921 cagaagctag tttagtaaaa acactagaag aactaggtat aggtagacca agtacgtatg
     4981 ctccaacaat agctactata ctgaatagag agtatgttga aaagcaagga acaagtctat
     5041 gtccaacaga acttggaata atagttactg atattttgga aaataatttc cataaattta
     5101 tagatgtaga ttttactgct gatatggaaa gtaaacttga tgttatagaa gaaggcaata
     5161 cagattggaa aaagattgta tcagaatcat atgcaccact taaagaggct attgaagaag
     5221 ctcttgaaaa tatagaaaaa gtaaatatgg atgaagaaac tgatgagatt tgcgagaact
     5281 gtgggtctaa tatggttatc aaatatggta gatttggaaa gtttatggct tgtaaaaact
     5341 atcccgattg taaaacaact agaccaatta tgaataaaat tggtgttaaa tgtccaaaat
     5401 gtgaagaagg agatattata cttagaaaat ctaaaagagg taaagctttt tatgggtgtt
     5461 ctaattatcc aaactgtgac tttgtagctt ggaataagcc aacaggagag ttttgtgaga
     5521 agtgtggttc atatatggtt gaaaaagtga ctaaatctga aacaaaaaca gtgtgttcta
     5581 ataaagaatg taataaagag acaaaagagc ataatgacgg aaaagagtga aaattgaaaa
     5641 aaatcacata attctcttga aaaaggttcc aatctatgat aatctttcaa gtgtaaacaa
     5701 ctaatagaaa aataaatttt cattaaatgt gaaaataata ttagaaaata taaaaggtta
     5761 ttaaaaggcc taaatttcat gaggagatga ttaaatggca agtgaagtgt tacaaaaaac
     5821 aaggaaaata aataaaacat tacaaacaag tggtggaagc agtgtctctt ttgatttact
     5881 ggccggagca ttgggcgacg ttttaagttc taatgtttat gtagtaagtg caaaaggtaa
     5941 agtactaggt cttcatttaa atgatgttca agacagttca gttatagaag atgagtatac
     6001 taagcaaaag aaattttcag atgaatatac tcaaaatgtg ttaaaaattg atgaaacatt
     6061 agaaaattta aatggtgaga agatattaga aatctttcct gaagaacatg gaagattaca
     6121 aaaatatact acagtagttc caatattagg aagcggtcaa agattaggaa cattggtact
     6181 ttcaagatat tcaaattcat tcaatgatga tgatttagta atagctgaat acagtgcaac
     6241 tgttgttggt cttgaaatat taagagcaat aggtgaagaa ttagaagaag aaatgagaaa
     6301 gaaagctgta gttcaaatgg caataggcac tctgtcctac tccgagcttg aagcagttga
     6361 acatattttt gctgaattgg atggaaaaga aggtctactt gtagcaagta agatagctga
     6421 tagagttggt ataactaggt ctgtaatagt aaatgcactt agaaaatttg agagtgcagg
     6481 tgtgatagaa tcaagatcat taggtatgaa aggtactcat ataagaatac ttaatgacaa
     6541 acttacagat gaattaaaaa aattaaaaaa caatcaataa tctatatttt ataggtttag
     6601 attagaaaag gtatctattt gaaatagata ccttttttag acgaattatt aaggtaatga
     6661 taagtatatt aaaaaggagt gagaaaatgc ctttagcaga taaaataaga ccaaaaacaa
     6721 tgaatgatgt aataggtcaa agtcatataa taggtaatgg aaaaatattg tctaagatat
     6781 tacaatctaa ttttttacca aatatgatat tttttggtcc tcctggtgtt ggtaaaacta
     6841 cagtagcgga aataatagca gaaagaagca ataaaagctt ctataaaata aatgctacta
     6901 attcatcttt agaagatata aaaaaggtga tatcagagct tggaagtata aataatgtaa
     6961 atggagtcct attatatata gatgaaatac aaagttttaa taaaaaacag caacaatcaa
     7021 tattagaatt tatggaaaat gggagtataa ctttgattgc tagcactaca gaaaatccat
     7081 atcattatgt atataaagct cttttaagta ggtctactgt atttgaattt aaacctttag
     7141 aaaaacttga tatagaaaaa ggactgaaaa gagctgttga agtattaaat gaagatagct
     7201 atatggatat tgagtgtaat aataatgcta ttgaagatat agcaattctt tctgatggag
     7261 atatgagaag agccttaaat attttagaag tggtagtcta ttcaactaaa ccaaataagg
     7321 ataatataac ttatatagat tcagatgtaa taagggcatc tacatttaat aaaattataa
     7381 actatgacaa aaatggtgat agtcattatg atattttaag tgcatttcaa aaatctataa
     7441 gaggaagtga cccacaggca tctatacact atctagcaag acttataaaa ggtggggatt
     7501 tgataagtat atgtaggaga cttttagtaa tagctagtga agatataggc ttagcttatc
     7561 caaatgctat tgtaattgta aaggcatgtg tagactcagc tatgcaactt ggatttccag
     7621 aagcgaaaat accattagca gaagctacta ttcttttggc tacatctcca aagtctaatt
     7681 ctgcatgtat agcaataatg aaagctatgg atgaactgga taaagagttt gttaaggata
     7741 taccaaatag tataaaggat gctcactatt ctggttctaa agaaataggt aggggttgtg
     7801 gatacaaata tcctcataat tttaaaaatc attatgtaaa gcaacagtat ttaccagata
     7861 gcataaaaga tagtatttat tacataccac aagaaaataa aactgagaaa aatataaaaa
     7921 aatatttaga atatctaaat ggtgattttt agactgaact ttatgtatcg ggggtagata
     7981 ttatgattat aaaacttaac tcaacttatc acagtagagt tatgaaatac ttaaaaaaag
     8041 aacctgagta caatttgttt ataatagggg atattgaaag atatggatat ggaaataatt
     8101 ttttaaatat atgggcagat gtgggtgaac atggtgaaat aaaggcaata ttacttaaat
     8161 attttgaatt tatgatgttt tattctgatg gagagtatga tgttgaaggt ttttataatc
     8221 tacttcgtaa tactaattac gaagaaatat ctgggaaaat atgtgctgta gatgctttag
     8281 caaagagatt aggacttaat aatttaaagg tagttgattt ttgcaaactt caaactaaaa
     8341 agtttttgat agataataat tgtaatgtaa aggttaagag aattagactt ggaaatctaa
     8401 aaaagacagt aaaactttat gacttaatag atgaatttca cagtacaact ttagagaatt
     8461 taaaaaatgg actaaggaca ggtagaggat attgtataga gattaacaaa caggtggtgt
     8521 caatggcaaa gagtacttca gaaaatagga ctcatgcaat gataattgga gtaggaacac
     8581 atcctaaata tagagctaaa ggacttgcta caaaatgcct aattaaacta tgttcagagt
     8641 tactgagaga aaacaaaatt ccttgtttgt tttatgataa tgaagaggct ggaaagatat
     8701 ataaaaaact aggatttgaa aacattggaa aatggggaat ttattcaaaa taaaaaatta
     8761 aaattaatct tgacaaaatt agtagggtat aataaaatta tcttgaaagt aattcctact
     8821 aaaacagtat gaattagatt tctatataga ggtgataata tatgaagttg tctactaaag
     8881 gtagatatgg actaaaagca atgtttgaat tagcattaaa tcaagacaat ggaccagtat
     8941 cattaaaatt tattgctaag aagcaaaaaa tatcagatca gtacttagaa caaatatttt
     9001 catctttaaa aaaatcgggt ctagtaaaaa gtgtgagagg tgctcaagga ggttatttgc
     9061 tttcaaagaa tgctgaagac ataacagtag gagatatact tgtagtatta gaaggaccag
     9121 ttgcattatc tgactgtgta ctagatgaag atgtatgtga aaactcaaac atgtgtgtaa
     9181 caaaaatagt ttgggaaaag atgaaaaagg gtatagaaga tgttatagat tcaattactc
     9241 ttaaagatat gataaatgac tataacaaaa ataaattaga aaatgatata acaaatatta
     9301 aaaaatagat ttggagatga atgtaatgga aaaaagaaga ttgtatatgg attattctgc
     9361 aacaacacct attaaaaaag aagtattgga tgcaatgatg ccatacctaa cagattattt
     9421 tggtaatgca tctagttttc acacattcgg aagagaagca aaagatgctt tggataaagc
     9481 aagagaacaa gtagcagccc ttataaatgc tgaacctagt gaaatatatt ttacagcagg
     9541 tggttcagaa agtgataact ggacactaga gggtgtagct tatgctaata aaaacaaagg
     9601 taatcatata ataacttcaa aaatagaaca ccatgcaata cttcatacat gtgaatactt
     9661 agcaaaacat catggatttg aaataactta tttagatgtt gatagcgaag gaaaagtaga
     9721 cttaaaacaa ctagaagagt caataaaaga tactactata cttataagta taatgtttgc
     9781 aaataatgaa ataggaacta tacagcctat taaagaaata tccgaaatag ctaaaaaaca
     9841 taagatatta ttccacactg atgcagtaca agctacaggt aatattccag tagatgttaa
     9901 agaattagga atagatttga tgagtatgtc ttcacataaa atatatggac caaaaggtgt
     9961 aggagcttta tacataagaa aaggtgtaag acttcataac tttgtccatg gtggagcaca
    10021 agaaaaaagc aaaagagctg gtacagaaaa tatacctgca atagttggat atggaaaagc
    10081 agcagaatta gctaaggaaa atatgcaaaa tcatgtagaa actttaactc gtcttagaaa
    10141 taagttaata gatggtgtgt tagaaagaat accttacaca agagtaaatg gtagcttaga
    10201 aaatagattg ccaggaaatg caaactttgc attccaattt atagaaggtg aaggaatact
    10261 tttattatta gatatgttag gaattgctgg ttcaagtggt tctgcatgta cttctggttc
    10321 attagaccct tcacatgtac ttcttgcaat aggtttacca catgaaatag cacatggttc
    10381 attaagactt acagttggag attttacaac agatgatgat atagattata tactagagaa
    10441 cttaccaaaa gtaatagaaa gacttagaag catgtcacca ctttatgatg atgcgaagaa
    10501 acaaggttta gtaaaataga cataaagata aagcataaaa aatagacata aattgagtag
    10561 gaggaaaaga taatgcaata tagtgataaa gttatggaac attttatgaa tccaagaaat
    10621 atgggagaaa ttgataatgc aagtggtgta ggtgaagttg gaaaccctac atgtggagat
    10681 ataatgaaga tatttttaga catagatgga gatgtaataa aggatgttaa gtttaaaaca
    10741 tttggctgtg gttcagctat agcaagttct tctatggcaa ctgaaatgat aaaaggaaaa
    10801 actataaaag atgcattaga acttacaaat aaagctgttg cagaagcttt ggatggttta
    10861 ccaccagtaa agatgcattg ttcagtttta gctgaacaag ctgtaaaagc agctcttata
    10921 gattatgctc aaaagaacaa tatccatata ccagaattgg atggatatgt tatagatgat
    10981 gctcatgacc atgatgttga agaagaagaa taaaaataag tgattgtgtt atgagaagtt
    11041 tacaatgatt aaaaagaagt actaatgata taattaggac ataaaaaata gctgttttta
    11101 aaatggctat tttttattgc ctaaattaag tattttaact tgatttagag gttaattagt
    11161 taacaaaata atccataaga ttcaaataat aaagatgatt atttttttag aaaatagaac
    11221 aagctatatt ttaaataagt acaatacttg ctctatatgg tataataata agtaatgcaa
    11281 aagatgaggt gaaatttgtt tatgaataaa aaagtaatga taggtatgag tggaggagtt
    11341 gatagctctg tagctgcata tcttttaaaa caacaaggat atgatgtcat tggtgttact
    11401 atgaagttgt ggcaagatga tgatgttgtt gaaattgaag gtggttgttg ttctttatct
    11461 gctgttgaag atgctagaag ggtagcaaat aaaataggta taccatttta tgttttaaat
    11521 tttagagaag tgtttaaaga aaaagtaata gattatttta tagatgaata tttggaagga
    11581 aaaactccta atccctgtat agcttgtaat aaacatataa aatttgatga tttttataag
    11641 aaggctagac aaataggttg tgattatgta gctacaggtc actatgcaaa gatagaaaag
    11701 gatgaaagta caggaagata tttgctaaaa aaatctgtta cagataaaaa agaccaaact
    11761 tatgcacttt ataatcttac acaagagcaa ctagagcata ctttattgcc aataggtgac
    11821 tatgaaaaag atagagtaag agaaattgct aaggaaatgg gtatggcagt acacaataag
    11881 cctgatagtc aagaaatttg ttttgttaaa gataatgact atgcaaatta tgtaaaaaaa
    11941 cattctaaaa agcgtattga agaaggtttt tttgtagata ctaaaggcaa tatacttgga
    12001 aaacataagg gtatattata ttatactata ggccaaagaa aagggcttgg aattactttt
    12061 ggaaaaccaa tgtttgtaat agacataaat ccaataaata ataccatagt tcttggagac
    12121 aatgaagatt tatttaaaaa ggaacttatt gctaaagatg ttaactttat atcaatagat
    12181 acgttagaag aaccattaag agttcaagcc aaaataagat attcagcaaa accatcacca
    12241 gctactattc atagagtagg agaagatact attaaaatag tttttgatga agctcaaaga
    12301 gctattacta aaggtcaatc tgtagtaatg tatgatggag atattgtagt aggtggaggt
    12361 ataatcgaga aaagtttata aaaacatcta aaaaattaaa aaaataaaca ataaagtata
    12421 ttaagagtaa ataaagttta taaaataaat ttattgtata tgaaaatata gtattgatta
    12481 attttgtaaa atttagtaat ataattagta tattaattaa aaaacaaata cagtgataag
    12541 gaatagtaaa atatttgatg tcttacagag agagggaaat gctggaagcc cttagaatat
    12601 gatatttgaa gcagcctttt agtagcaatc ttgaacaatt tatattttat aaagaagatt
    12661 taagtagaga ttgacggtag ttacgttata gctaaggttt tccgaagata tcatactttg
    12721 tatgataatt agggtggtac cgcgattgtc ctcgccccta taaatttatt tatagggacg
    12781 aggtttttta tttaaacgaa aaaataaaat ttattaggag gaacatgata tggaaaagat
    12841 gggattaaat gaaataagaa gtaagttttt agaatttttt gagtcaaaag gtcattatgt
    12901 agcaaatagt tattctttag taccaaacaa tgataagagt ttgttactta taaactcagg
    12961 tatggcacct ttgaaaaact acttttcagg tgtagaagta cctccaagtg tgagaatgtg
    13021 tacatctcaa aaatgtataa gaacaggaga tatagaaaat gtaggtataa ctgcaagaca
    13081 tgctacattc tttgaaatga tgggtaactt ctcatttgga gattacttta aaagagagtc
    13141 aatcaaatgg ggatgggaat ttgttacaga atggctaaat ataccagaag ataagatatg
    13201 ggtgactgta tatgaagaag atgatgattc ttatgatata tgggcaaaag aaatgaattt
    13261 cccagaagaa agaatggtta gacttggaaa agatgataat ttctgggaaa taggtacagg
    13321 tccttgtggt ccttgttcag aaatatattt tgatagagga gaagaatatg gatgtgacaa
    13381 tccagactgt aaaccaggct gtgattgtga taggtattta gaattttgga accatgtatt
    13441 tactcaattt gatagagatg aagaaggaaa ttatagtcta ttagaaaaca agaatataga
    13501 tacaggtatg ggtcttgaaa gaatgggttg tattatgcaa ggcgtagaca ctatctttga
    13561 agttgataca attaaatcta tactagaagc agttgagaag ttaacaggag taaaatatgg
    13621 agagaatcca aaaaatgata tatcaataag aataataact gaccatataa gagctgtaac
    13681 attcttagtt agtgatggag tgcttccatc aaatgaagga agaggatatg ttcttagaag
    13741 acttcttaga cgtgctgctc gtcatggtaa gttattagga gtaaaagaat tatttttaca
    13801 gaaactaata gatgaagtta taaaagtaaa tgataaagca tatccagttt tagttgaaaa
    13861 agaaagttat attaaaaaag tagttggaat agaagaagaa aaattcaatg aaactataga
    13921 ccaaggaaca gaaatattga actcttatat agaagtatta aaaaatgaag gaaagactgt
    13981 cctaagtggt caagaagctt ttaaactata tgatacttat ggcttcccta tagacttaac
    14041 taaagaaata ttagaagaag aacatctatc tgtagatgaa gaagctttta atgaagaaat
    14101 ggaaaagcaa aaagaaagag ctagaaatgc tagaggaaat atggatggag aaagttggaa
    14161 ggaagaccct ctatcaaaat tagaatctac agttgacagt acatttaatg ggtatagtga
    14221 aatttatggt gaaggaacta ttgaagctat agttaaagat gatgagctag ttcaaagtgc
    14281 agaagaaggc gataaggtat ctatagtact agataacact actttctatc ctgaaggtgg
    14341 aggacaagtt ggagattgtg gattgataac taatgaaaac ttagttttag aagtattaaa
    14401 tactaaaaaa ggtgctaata atagtataaa acatataggt ataataaaat caggaagaat
    14461 aagtaatggg gacaaagtaa agacactcgt agatagagaa actagaatgt cagctgcaag
    14521 aaatcacagt gctacacatt tacttcataa ggcattaaga gaagtattag gagaacatgt
    14581 taatcaagct ggttctttag taactccaga aagacttaga tttgatatta ctcactttga
    14641 agcaatatca aatgaagaat taaaagtaat agaagaaaaa gtaaataatg ttattttatc
    14701 atctttagac ataaaatgtg atattatgaa tataaaggaa gctaaagaaa aaggagctac
    14761 agcactattt ggtgaaaaat atggtgatga ggtaagagta gtttctatgg gagattattc
    14821 tacagaactt tgtggaggaa ctcacttgac aaatacttct caagtaggaa tgttcaaaat
    14881 attatctgaa ggtggagtag cagcaggtgt aagaagaata gaagcaataa caggaaaagc
    14941 tgtttatgaa tacctaaaag aaagagatgg aattatctct gaagtttgtg taaacttaaa
    15001 atctaaagaa gataatttaa ttcaaagaat aagttcttta ttagaagaaa ataaaaactt
    15061 atctaaagaa ttgcatgata tgaaagctaa gatgagttta caatcagttg attctatttt
    15121 tgattctaaa gttgaagtaa atggagttaa tttgataact aataaatttg aaggtatgga
    15181 tatggataca ctaagagaaa cagcagataa tctaagagat aagcttggtt ctggtgtagt
    15241 agtacttgca aatgtagtag atgataaagt taactttgta gttactgcca ctaaagatgt
    15301 attagataag ggaatacatt caggtaatat tgttagagaa gttgcaaaaa tagctggagg
    15361 aaaaggtgga ggaagaccta atatggcaca agcaggagct agtgatgtat ctaaggttga
    15421 ccaagcttta agctatgcaa gtgaggttat taagacacaa gttaaataac attcactcaa
    15481 ggaggttttt agtttggaaa aagatttaga ctacactatg aaatttgaag gaataccaga
    15541 agataggatg agtgtaggag atactattga ttttgtatat aaagcattag ttgaaaaagg
    15601 atataaccct ataaatcaaa ttatagggta tctcctttct ggtgattcta gctatataac
    15661 tagtcataaa aatgcaagag ctataattaa aaaatttgaa agagatgaga tacttgaaga
    15721 agtaatcacg cactatctga atagaaagta ggtcaaataa atgagaaaaa agattatgtc
    15781 gttgtttgca atagtcatta tgatttttgt atcaactgct agctatggtt ttgctgaaga
    15841 aaaaggcaag gtcatattta ttgatatgaa taggaccaat cttggaagta tgatgaatat
    15901 tccaatcttg aaaaatgaga tagaaaagag aggctatgta gctctcatga atataagagg
    15961 ggatcaaggt actgatgata gaaggtctta tgcaagtatg ggtgcaggaa caagagctag
    16021 tgtaactact gaagactata tcaattttca aaaagtagac caagatactg ctaaagcttt
    16081 tgaagtcgca acaggtcaaa aagctaaaga aataaatgat ttaggcatta atagatctat
    16141 aaatgaaaat cttgagtatg gagaatatgg agcaaattta gggtcattag gaaaaacttt
    16201 atcagaaaat ggtttaaaga catctgtaat tggaaatgct gatataattg aaaataatca
    16261 attagttaaa aacagaaact tatgtttagt agctatggac caatatggaa ggattgattc
    16321 aggtaatatt gataatatta ataagaaaga ttctaaaatg ccatttggaa tatctacaga
    16381 ttatgataaa ttactagatg aaactaaaaa atcctataaa aatagtgatg ctatatttgt
    16441 agaacttgga gatacatata gattagatat gtatagatta aatctaaatg ataatactta
    16501 tgatacaatg aagaaaaata ttgtaaaaaa tataaataaa tatcttgaac aagtattttc
    16561 tatggttgga gaaaatgaca ctgtttatat agcaagtgca tttccaagtg acctagatta
    16621 taagaataag agaagacttt ctccaataat aaaatttagt ggagaaggga aaggtctact
    16681 ggaatctgcc acaacaagaa aagctggaat tgttgccaat ttagatgttg gagtagatat
    16741 actaggtaca tttggtcttg aaaataaaga gatggtaggt agaagttata ctttagtaaa
    16801 taaagaggac aatattagct atctaaaatc actttatgaa aggatagtgt ctatatcttc
    16861 aataaggtca actattataa atggctttgt aggcgtaata tctgcttctt gggtaattgc
    16921 catgtttgcg attttcttta gaaattatat gccacataaa gagcgtgtat tcaaagtact
    16981 taaggagttt ataaaattag gcataataat gccactatca tttatgttag ctcctttgat
    17041 gaattttaaa actccaattt caatatcttt gggaatattt gcaactacgc taatattata
    17101 cttgttaggt agaaaattat ttaaggacaa tgatataaag cacatgggat tttttgcaac
    17161 acttactatt ttagtcatag ttatagattc tgtatttgga acatacttaa tgcaaaataa
    17221 cataatgagt tatgatgctg tcataggagc taggtactat ggtgtaggaa atgaatatga
    17281 aggtgtaact atagcaagtg cagtatttgg cttagcagta ttattacatt ataagaaggt
    17341 atctaaatta cttgttgtaa ttttttcatt aataatactt ataactagtg catatccttc
    17401 aatgggtgca aatgttggtg gagctatatc agaatgtgta gcatatttgt tgtttataat
    17461 gcttatatat gacattaaaa tagattttaa gaaagctgta ttattagctg tttcagctgt
    17521 agttgtcgtt tttgcatttg cagctttaga tatactttca ggtagtgaat ctcatttagg
    17581 tatgtttgta cagagaattt tcctaaatgg accaggagag atattacaaa catttggaag
    17641 aaaaattcaa atgaatatgc aactagcaca gacaagtgta tgggtaaata ttttacttgt
    17701 aggaattgga gtaattgcag tgcttatatt taaaccatct agacacttta gaaagataat
    17761 gaataatttt ccaatcttgt ttaaagggtt tattgcatct atggttggtt gtataattac
    17821 tcttttagtt aatgattctg gaatagttgc tgcatctaca gcatctatat atatacttat
    17881 accactaata ataataagta taaatatgat aatatttgaa aaaaataact aaaaaaattt
    17941 ttactgttca tataggattt aaataaaaaa tgcaatatga ttattaatgt aaactggatg
    18001 cttacataaa aaatcatatt gcaattttta tgattgatta tgtaaaatta tatgaagcag
    18061 taagcctgtg accttagtta tgagaggttc agaatatgta tttaatctat agaatataaa
    18121 tagataaagg aggaactatg ttagatggaa gaataatggg tcttgatgtt ggtgataaaa
    18181 ctattggagt tgctgtgagt gatttgatgg gattaactgc acaaggtgta aaaacaataa
    18241 aaagagttgg caagaaaaaa gatattgaag agctaaaagc aataataaaa gaaaaacaag
    18301 taaataaaat tgtatcagga ttaccaaaaa atatgaatgg aactttaggt ccacaaggag
    18361 aaaaagtaat caagttttgt gaattggtaa aagcagaaac aggtattgat gtagagtttt
    18421 gggatgagag gctttctaca gtagcggcag agaggtcttt attagaagca gatgttagta
    18481 gacaaaaaag aaaaaaggtt atagatatgt tggcagcagt aataattttg caaggatatt
    18541 tagattttaa aataaattca taaaagaact ataaaattga atacttatga tatataatat
    18601 aaataataaa aataaaaata aatttgaggt gaatgacatt atggaagaaa atataataaa
    18661 tttaatagac gaaaatggcg ttgaaagcca atttgaaata atattaacat tagaagcaga
    18721 aggaaaagag tatgcaatat taatgccttt agatgatgaa gaagcagaag aagctttaat
    18781 ctttagaatt gatgaggatg aagaaggtga aatcttaata cctttggaaa gtgatgaaga
    18841 gtatgaaact gttgtagctg tgtatactgc tataatggaa gaagaaggtt taaattacga
    18901 cgaagatgaa tcaaatggac ttaactagat aagtaattaa gtttgtatat aaaaatcaga
    18961 tatttattat atttgcttta attaaacctt attaattgta aaagcattta tagtatatat
    19021 ctgttttttt gttttttatt aaaaatacca aaaaatttat gaattttggt gaataatata
    19081 tcttttatgg gtatatattt atattatgag aataaattaa atttatttaa atgtcttgac
    19141 aaaaaatgaa ttatcaatta caatattatt taataaagat tatcaattga aaatagtaag
    19201 ggtgatgtta tggcaaatac aatggattta ttaaaagaca agttaaaaga aactggtttt
    19261 aagattacgc cacaaagaag agcaatagtt gaaatattgc ttaaacatga ccattcacat
    19321 ctaagtagtg aggaaatcta tgatttagtt agagttgact gtcctgaaat aggcttagct
    19381 actgtatata gaacaatgca gctattggat gaaataggat taatatcaaa actaaactta
    19441 gacgatggat gtataagata tgagattagt ctacataaag aagactgtca taatcatcat
    19501 catcttattt gtaaaaattg tggaaagata atggaagcta aagaagacct tttagacaat
    19561 atagaaaaag agatacaatc attgtacaaa tttaaaatac ttgatcatga tgttaaattt
    19621 tatggactat gtgacgagtg taatggagta agcgattcag aagaataaac ttctattgct
    19681 taatgtcgat ggaagaatag tatagaactt ttatattcta tgctattctt ccatttttta
    19741 ttataaacca tctataaaat gcaaatatac agttttcaag atgcttatag ttttaatatt
    19801 gaaaatataa ttaatcctat aagtacaaaa atgaatgatt tttctatgaa attactaaat
    19861 ggtcctccaa cagttaaaaa acctaaagaa attttctttt cgcttagcgg ataaaaaatt
    19921 ggtattcctt gctttgtaaa taaatctcct aaaaacatat gacttgcata accaatcgta
    19981 ccataaaaag ataaattatt aatattagtg attaattcta tctgttttag taaaaaatat
    20041 actattatag ttgcaaatat gctatgagaa aaacttctgt gctttaacca gggaaaaatt
    20101 gacaacatta gacaaaaaat tgtaaaatat atttcaactt ctatgatgta taagcatata
    20161 gctaatagta taaatattaa tgatagaaaa acttttctta gaaatgtgtg ggttagtttt
    20221 gcttcaataa ttataattgc aacaaatgta attattgcgc ttaaataaaa attatcattt
    20281 atatttattg aaataaagaa aataataata ttaacagcat atataaaaat tctatatatg
    20341 tatttggata cattgttttt tagaatgaaa tttgagacca tagaattaga tttatccaaa
    20401 tcgggtagta cagcaaaagt agctgaaact aaaatatcaa ctaaagatat aggaatttta
    20461 aatattaaag atgtttggat agctgttaag attcctattg cacaatgagt tttaccacgc
    20521 ataaatcctc ctaaattatc cgatattaaa tcatataata tttagaggat atcaaaataa
    20581 aataggtttg tcaaaataat acgttaaatg tattttaaat atagcttaaa atatgatacg
    20641 aattatggta caataagaat aactggtagc gagtgcgtga tttgatatta tttatgattg
    20701 tagaacgtgt taaaaaatta aatagacaga aggagatgat gcaattgttc aaaaagaaca
    20761 ccaacaagat aaaggtaatg gccttgggtg gacttaacga agttggaaaa aatatgactg
    20821 ttgttgagta taaagatgaa ataattgtta tagatgctgg acttagtttt ccagaggatg
    20881 aaatgttagg agtagatata gttataccag atataactta tttagtaaaa aatagggata
    20941 aaataaaggg tatatttatt acacatggac atgaagacca tataggagct cttccatata
    21001 tattaaagaa aataaatgta cctgtatatg gggcaagact tagtataggt cttatacaag
    21061 taaaacttaa agaacataaa atgaataatg ttaagttaaa tgttataggt cctagacaag
    21121 ttataaagct tgataatatg gaagtagagt ttttgaaaaa taaccatagt ataccagatg
    21181 cttactctat tgcaatacac acagaccaag gtataatata ccacacagga gattttaaga
    21241 ttgacttgac tcctattgat ggagatgtta tggatatgca tagaatctgt gagttgagca
    21301 aaaaaggagt tcttttaatg ctggcagata gtactaatgc agaaaaacca ggatttacta
    21361 tgtctgaaaa gactgttggg gtaggtcttg acgaattatt tgcaaaagga aatggcagaa
    21421 gaattatagt tgctaccttt gcatccaaca tacacagatt acaacaaata ataaatactg
    21481 ctgaaaagtt taatagaaaa gttgctatat caggtcgctc aatggtcaat gtagttggag
    21541 ttgcaaaaga attaggatac ctagatattt ctgatgacat gcttatagat ttaaatgata
    21601 tatgtaagta tgaagatagt gaattagtga taataacaac tggttcacaa ggtgaaccta
    21661 tgtcagctct tgctagaatg gctttttcag agcacaaaaa agtagaaata aagagtggcg
    21721 atttagttat aatatctgct catccaatac caggaaatga aaaattaata tcaagagtaa
    21781 tcaatttctt atttgaaaaa ggagcagaag tagtatacag tgatatagct gatatacacg
    21841 tttctggaca tgcctgtcaa gaagaattaa aacttataca tgcattagtt agaccaaagt
    21901 tttttatgcc agctcatggt gaatatagaa tgttaaaaag acatgctgaa atagctgaac
    21961 agcttggtat ggacaaagaa aatatttttg tcatgcaaac aggagatgtt ttagaattag
    22021 ataaaaattc tgctaaggta gcaaaccgta tacaaacagg aaacatatta gttgatggtc
    22081 taggagtagg cgatgtaggt aatatagttt taagggatag aaagcattta tcagaagatg
    22141 gtcttatgat agttgtagtt actatttcca aggatgaggg taaggtctta gcaggtccag
    22201 atataatttc cagaggattc gtatatgtaa gagagtcaga agatttaatg gatggagcaa
    22261 aggatataat taagaatgtt ctaaatgaat gtgaagagaa aaatattaaa gaatgggctt
    22321 atttaaagaa taatattaaa gaaaacctaa aagaatattt atatcaaaaa actaaacgaa
    22381 atccaatgat acttcctatc ataatggaag tataatataa aaaaactata aatttgaaaa
    22441 ttagacctct tgtaattttt aaagtaatat caaaaatcca attgatatta tcttaagaat
    22501 tacaagaggt ctttttaata ctcattttaa aaaaaaatat attacataaa ataccaatta
    22561 taacaatctt ttattagtaa aaactaatta tatataaatt ttactgatag gaggattctt
    22621 atggatgatg tttcaagaca aaatgctata aaagctttaa aacaaacaaa aatggaaata
    22681 gcaggagagt atggaatgaa ttatgaagat gcttttgaaa taatagaaaa tgcgtctaat
    22741 aaaggtgttt tagaaggtta ctttaaaaag cttgaaaaga aaaaaaattt aggtcaaggt
    22801 atatctaggc atttagaata aaaaaagtta aatgtgagaa aataagttta aataaacttt
    22861 aagaggtgac atcgatgaaa aaattagcta gtactgcttt ggctatatta attgcgctta
    22921 ctcctttaag cttttctttt gcaaataata aggaaaatgc tgatgccaac caactaaata
    22981 tatcttcaaa atcagcaatt ttgatggatg ttggtagtgg acaaatttta tatgaaaagg
    23041 atgctcataa aaagcttcct cctgctagtg taactaaggt aatgactatg ctacttatag
    23101 tagaagcttt ggattcaggc aagattaagc ttgatgatga agttcaagta agtgaaactg
    23161 catctagtat gggtggtagt caaatattcc tagagccagg agaaactcaa aaggttgata
    23221 cattattaaa aggtatagct gttgcttctg caaatgatgc ttgtgttgct atggctgaac
    23281 acttagctgg aagtgttgaa ggatttgttg atagaatgaa tgctaaagca aaagagttaa
    23341 atatgaatga tactcatttt gcaaatacaa atggacttcc agttgcaaac cactatacat
    23401 ctgctcatga tatagcttta atgtctagag aactattaaa acacgaaatg ataagtaaat
    23461 acttaactac atggatggat aaggttgtag tagggaagaa acaagttaca gtaggtcttg
    23521 caaatactaa taaacttata aagcattatc aaggagcaac tggtgttaaa actggtttta
    23581 ctcaagaagc aaaatactgt ttatcagcat ctgcaaagag aggtaatact catctagttg
    23641 ctgcaacttt aggagcagag acttctccag aaagatttaa tgatgcctct agccttttaa
    23701 actatggatt tgctaattat gaaagtgtta agttatgtag taaaggtgat aatatagcaa
    23761 cacttacatt ggataaggct gatgaaaaca aagttaaatt ggttgctaaa gaagatttaa
    23821 atactcttat taaaaaagga agttctaaag aatttgagaa gaaaattgaa atagtaaaaa
    23881 atcctaagat gccaattaaa aagggaactg ttttaggaaa aataaagata tgtaaagata
    23941 aaaaagtaat aggcgaagtg gaattgataa atactaagga tataaataag gcaagctatc
    24001 tacaaatgct tcaaagaata attgataata tgatataaat aattatcaga tgaaatataa
    24061 ttataataca caaagatatg attataatac aca
//
LOCUS       DAENCV010000053        23685 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAENCV010000053 DAENCV010000000
VERSION     DAENCV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.053"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(112..306)
                     /locus_tag="KQD82_002130"
     CDS             complement(112..306)
                     /locus_tag="KQD82_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380600.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWVDRVRANSYSYENKIAK"
     gene            complement(615..2144)
                     /gene="lysS"
                     /locus_tag="KQD82_002131"
     CDS             complement(615..2144)
                     /gene="lysS"
                     /locus_tag="KQD82_002131"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG4380601.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2159..2638)
                     /gene="greA"
                     /locus_tag="KQD82_002132"
     CDS             complement(2159..2638)
                     /gene="greA"
                     /locus_tag="KQD82_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG4380602.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2747..3715)
                     /gene="dusB"
                     /locus_tag="KQD82_002133"
     CDS             complement(2747..3715)
                     /gene="dusB"
                     /locus_tag="KQD82_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG4380603.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3852..4622)
                     /locus_tag="KQD82_002134"
     CDS             complement(3852..4622)
                     /locus_tag="KQD82_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG4380604.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4641..5231)
                     /locus_tag="KQD82_002135"
     CDS             complement(4641..5231)
                     /locus_tag="KQD82_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG4380605.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5414..6382)
                     /locus_tag="KQD82_002136"
     CDS             complement(5414..6382)
                     /locus_tag="KQD82_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG4380606.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6375..7352)
                     /locus_tag="KQD82_002137"
     CDS             complement(6375..7352)
                     /locus_tag="KQD82_002137"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG4380607.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7667..9637)
                     /gene="ftsH"
                     /locus_tag="KQD82_002138"
     CDS             complement(7667..9637)
                     /gene="ftsH"
                     /locus_tag="KQD82_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG4380608.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9671..11059)
                     /gene="tilS"
                     /locus_tag="KQD82_002139"
     CDS             complement(9671..11059)
                     /gene="tilS"
                     /locus_tag="KQD82_002139"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG4380609.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDNFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDCIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11242..11670)
                     /locus_tag="KQD82_002140"
     CDS             complement(11242..11670)
                     /locus_tag="KQD82_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG4380610.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11687..12493)
                     /locus_tag="KQD82_002141"
     CDS             complement(11687..12493)
                     /locus_tag="KQD82_002141"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG4380611.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12695..13180)
                     /locus_tag="KQD82_002142"
     CDS             complement(12695..13180)
                     /locus_tag="KQD82_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG4380612.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13231..13890)
                     /gene="spoIIR"
                     /locus_tag="KQD82_002143"
     CDS             complement(13231..13890)
                     /gene="spoIIR"
                     /locus_tag="KQD82_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG4380613.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14035..14715)
                     /locus_tag="KQD82_002144"
     CDS             complement(14035..14715)
                     /locus_tag="KQD82_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4380614.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14699..15589)
                     /locus_tag="KQD82_002145"
     CDS             complement(14699..15589)
                     /locus_tag="KQD82_002145"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG4380615.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15707..17257)
                     /locus_tag="KQD82_002146"
     CDS             complement(15707..17257)
                     /locus_tag="KQD82_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG4380616.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17351..17617)
                     /locus_tag="KQD82_002147"
     CDS             complement(17351..17617)
                     /locus_tag="KQD82_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG4380617.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17765..18625)
                     /gene="yabG"
                     /locus_tag="KQD82_002148"
     CDS             complement(17765..18625)
                     /gene="yabG"
                     /locus_tag="KQD82_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG4380618.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18709..19647)
                     /locus_tag="KQD82_002149"
     CDS             complement(18709..19647)
                     /locus_tag="KQD82_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG4380619.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19757..19918)
                     /locus_tag="KQD82_002150"
     CDS             complement(19757..19918)
                     /locus_tag="KQD82_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380620.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19928..20425)
                     /locus_tag="KQD82_002151"
     CDS             complement(19928..20425)
                     /locus_tag="KQD82_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG4380621.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20540..21250)
                     /locus_tag="KQD82_002152"
     CDS             complement(20540..21250)
                     /locus_tag="KQD82_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG4380622.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21426..21956)
                     /locus_tag="KQD82_002153"
     CDS             complement(21426..21956)
                     /locus_tag="KQD82_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380623.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21973..22203)
                     /locus_tag="KQD82_002154"
     CDS             complement(21973..22203)
                     /locus_tag="KQD82_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380624.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22218..23612)
                     /locus_tag="KQD82_002155"
     CDS             complement(22218..23612)
                     /locus_tag="KQD82_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG4380625.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
BASE COUNT         7187 a         4608 c         2305 g         9585 t
ORIGIN      
        1 gttttttatt ttaaaaaagg ttataacttt tggttttttt taataaatat aattaaaact
       61 aatagatata acccttgata ttacaaacaa tgcatgcata tataatttta cctattttgc
      121 aattttattt tcataagaat atgaattagc tctcactcta tctacccaaa catttatact
      181 cttaacttta ttaacattag ttgtaccctc aaaaatatcc attgcatcct tacaaaaata
      241 tatcataaca tcaagaacca aaaatccaac acaaaataaa cataaaaaat taattacttg
      301 aaacatcaga cattccccca ataatttttt atatatagta ttctcataaa ttattctttt
      361 taccttctca taaattattc taaatatttt cataataaat tactttcaat catattttct
      421 aaatataaag tctcaatcaa caaaaactaa tatcaatttc taacgtttgt tggtaattta
      481 taaattaaat atctaaaaga atctttgaag catataaaaa gcattctaca ctgacatttt
      541 aaataaaatt tagctattta ttgataattt aatagctaaa ctttatttaa aacttagtag
      601 aatgctaata tttcttagtt ttcttcttct ttatttggat tgttatcaat aggtttcata
      661 gtcgggaata ataatacatc tcttatagat ggagagttag ttaataacat aattactcta
      721 tctataccta ttcctagtcc tcctgtagga ggtagaccta cctctaatgc ttttaagaaa
      781 tcttcatcca tttcaaaagc ttcatcatca cctaattctc tttttcttaa ttggtctata
      841 aatcttcctt tttggtcaat agggtcatta agttcagaaa atgcattagc taactcccac
      901 ttattagcaa atgcctcaaa tctatcagtt cttcttgggt cttcaacatt tctcttagat
      961 aatggagata cttcaactgg atgatgagtt ataaatgttg gttggattaa tttatcttct
     1021 ccaaattctt caaagaaagc atttattact tcccctcttc tcattccagg ttttatctct
     1081 ataccttttt ccctagcaac ttctagtgcc tcttcatctg tatttatagt agaaaaatct
     1141 acaccagaat attctttaac acaatcttcc atactcattc ttttccaagg tggtgtaaag
     1201 tttatttctg ttccttgata gttaactatc atacttcctg tagcaacttc agccatatgt
     1261 gcaataacat tttctgttat ttccatcata tcagtatagt ctgcatatgc ttgatataat
     1321 tctattgcag tatattcagg attatgtttt aaatccatac cttcatttct aaacattctt
     1381 cccatttcat aaactttatc aaatccacca acaataagtc tttttaaata caattcattc
     1441 gctattctta aatacattgg tatatgtaaa gtattatgat tagttataaa tggtctagca
     1501 tttgccccac ctgctatagt atttaatatt ggagtctcaa cttctaaaaa tcctctatca
     1561 tctaagtatg ctcttaatgc ttttaatgct tttgttctaa ttaaaaaagc attttttact
     1621 tctggattta ctattaagtc aacatatctt tgtctatatc ttagttcttg gtcttttaat
     1681 ccatgatatt tttctggtaa aacttgtaaa gatttacata gtaatactac agatttagcc
     1741 tttacagata tttcaccttt tttagtttta aagatttctc cttcaattcc aactatatct
     1801 ccaatatcat atgttgaaaa tacactatat tcttcctctc ctatagcatc tagtcttaca
     1861 taagattgga ttcttccctc ttggtcttga atatctataa agccagcttt tccttgtatt
     1921 ctcttgctca ttatacgtcc tgctattttt gtagttttac cttcaagaga atcaaagtta
     1981 tcttttatat ccatagaata atgagttcta tcatatctac tttctttaaa aggatctcta
     2041 cctgactctt gtaatttttt tagtttatct cttcttactt gaagtacttc actaaggtct
     2101 tcttgaattt gcgcttcttc attgctttgt tgattatttt tcattttttt cctccaaatt
     2161 atcttcttat ttctaatatt tcaaacttag caactccatc aggaacttgc acttccacaa
     2221 cttctttcgc agctctacct aaaagtgctt tccctacagg agattcgttt gatattttac
     2281 catcatatgg gtctgcttct gtcgaaccaa ctatagtata ttcaacctct tcatcaaatt
     2341 ctaaatcctt aactttaact attgatccta ttgtaacaac attaaggtct attttgcttt
     2401 catctatgat aacagctttt ctaaccatat tttctaattt taaaattctt tcttcaactt
     2461 gtgcttgttc ctttttagct tcatcatact cagcattttc agataaatca ccaaaagata
     2521 tagcaacttt tattctctct gcaacttctt ttctctttac aacctttaag ttttctaatt
     2581 cttcttctaa tttatcatat ccctcttgtg tcaataaaaa ctctttattt tcttccatta
     2641 taaatacaac tccttcacaa atttcttagg aattttccta attatatttt aaaaatttta
     2701 taatcttttt ttaatttatg cgctatatta taagtcacta ttatttttat gtcaaggagt
     2761 cttccatata gtactccaat ttcgacacaa cttcttgata gtcttctatt ttgttaattt
     2821 catcccttaa ttttgcagag tttctaagtc cttttaaata ccaagcgatg tgttttctca
     2881 tttcccttac agctacatac tctccatgtt cttctactgc aagttttaaa tgcttaatgg
     2941 cagtacttat tttttcgtta agagttggct ctggcaaaat ctcgcctgta ttcatataat
     3001 ggtttattct tttaaatatc caagggttac cttgagcacc cctacctatc attatagcat
     3061 cacaaccagt tttgtctaac atatttattg aatcttctat tgtaaataca tcaccatttc
     3121 ctataacagg tatactcaaa tttttcttaa tctctgcaat tatatcccag tctgctttgc
     3181 ctgtataaaa ctgttctcta gttcttccat gtattgctaa agcacttatt ccacaatcct
     3241 ctgctatctt tgctatttca acagcattta cactattatc atcccagcct tttcgaatct
     3301 ttaaagtcac tggctttttt gagttcttca ctactgccct caatacctct tctgcaagct
     3361 taggattttt cataagtgct gagccatctc catttttaac tacttttgga gctggacacc
     3421 ccatatttat atcgagaatc tcatttgaat aatcattcat tatctctgct gctcttccca
     3481 taaactctgg ttctgaaccg aatatctgaa ctgctacagg atgttcttcc tcttctatct
     3541 taagcatttt tttagtattt tcatcatcat agcaaagtgc cttaccattt atcatttctg
     3601 tatataaaag cccacatccc tgttctttac aaattaatct aaatggaagg tctgttacac
     3661 ctgccatagg tgatagaaaa actttatttt tcaattctaa atttcctatt ttcattgttt
     3721 ttctcctatt tttgccttta ttttgtgatt taactttaaa ttcttatttc ctcattataa
     3781 tataccacat atagctagaa atctatcata aaaagggaat tggatatata acccaattcc
     3841 ctgtatatta attattcttg gtttttttca tatattattc tcaatccttc tagtgttaaa
     3901 aaaccatcta cataatctat acttttcgtc tctgaagcaa tcagtttagc taatccacct
     3961 gtagctataa ccttaacatc atcacaattc aattctttct tcatcatact tattattttg
     4021 tcaactaaac caacatatcc ataaattatt ccagattgca tggctgatac agtactctta
     4081 caaatagtca tacctggctt agctaattct actctaggta atttagacgc actttgaaat
     4141 aacgcctcac tagatatttt tattcctggt gctattgttc cacccaaata ttcacctttt
     4201 tcagagatag cacaaaatgt agttgctgtt ccaaaatcaa caagtatact tggtgctcca
     4261 tacttttcta tccctgctac agcattaact attctatctg ccccaacttg ttttggatta
     4321 tcatatttta tatttagacc tgtttttatg cctggaccta ctattaatgg ctgtttttta
     4381 cagtacttta tacaaaagtt ttcaagagaa tgcataacat tcggaactac agatgatatt
     4441 ataacatcat caatatcact tatattcaca ttatcataat caaataggtt acttatcagg
     4501 attccatatt catcagacgt tttttcccta tctgttttaa ttctccagta attaactaat
     4561 ttgtcacctt tatatatacc taaaaccata ttagtatttc caacatcaaa tactagaagc
     4621 atatgtcatt aaccttcttt ctattttttg cttttcttaa gtacagtggc aacagcagct
     4681 actactaaag cccctactat tgcttctggc accccatttg ctgcggcaag agccattatt
     4741 acttttcctg ctgaaccact tttacccatt gcatgaagat acttgtcagc atataagaca
     4801 tatatcattc ctaaaactcc agctgtattt aacagagaac ctattgcccc tgtaattaaa
     4861 cctgatatgt ataccttatt tgtagcctta attattaact gatatacata ataagctaat
     4921 acacctatca taactcttgg taatattgaa actaatggat ttataaaaac aaaagatgtt
     4981 actgttgggt ttgttatagc atttaagaga cttgatatcc caaatataaa tcctattgct
     5041 gccccaacta ttggtccttc cataatagct ccaataataa ctggtatgtg cattatagtt
     5101 gcttttgtag gtccaactgg aataaatccc aatggagtca tagatagcat aatagaaata
     5161 gctgatagta ctcctattat agtcatcttt cttacattta cttttcttac tgatgcactt
     5221 gtgttcatca taaaaaatac acctccgttc cagctcatta agatgccgga ctacttaatt
     5281 ttttaatttt ttcgtttttt tcttataatt atgagtcaag ctaccattgt aggatgcccg
     5341 ctctaacaag aataatttta tcaagatgag ttgccattgt caacaatttg acaagaaaaa
     5401 tatgctaatt tcatcatatt tctcgttttt ttactatatc tttatatgcc attatatttt
     5461 ctacactatc cgcttttttt actgcattta ttatactttt ttccacaacc tctacagcca
     5521 atgaacctac taatgttata tctgcttcta tttctcctgt tgatattgtg aatattgtat
     5581 ctccatcatg aggtgtatgg ataggaaaaa tagacttagc atatccatca tgtcccattt
     5641 gagatatctt tttacatcca gctttggtta acttagcatt tgttgcaatt atcccaattg
     5701 tagtattatc tacactaaat ccacctttac tttcaccttg tttcattaat ttatatgtat
     5761 caattgtttc tgtcctttta tcatttaaag caccagctat aaccttacca ttttcataaa
     5821 catctccaag agcatttaca gcaattaatg cagatacaac tagaccatta tctaatttta
     5881 tagaatagct acctattccg cctttcatag catattcctg acctcttatc tttccaacag
     5941 tggctccaca acctgctcca taatttcctt gatttaaaat atcagtattt gcaacttcac
     6001 aagctctata acccatcttt aaatctggtc tacatttaaa gctccctact cctaaatcaa
     6061 ataaaactgc tcctacaact ataggcactt tacctacacc tacatcaaag ccaattcctt
     6121 tttcttctaa gtatctactt acaccacaag tggattcaag accatatgca gaacctccag
     6181 ccaaaactac tgcatgaact ttttgaacca tatttacagg gtctaataaa tcggtttctc
     6241 tagtccctgg agcagagccc cttacatcaa caccgcaagt agctccttct tcacatatta
     6301 ctactgtgca cccagtaaga ccatttctat cctcaacctg cccaaccttt aagccttcta
     6361 tatccaaaat attattatac atatcctttt tctcctctta tagaaacttc acctgaaata
     6421 atttctttta tacttccatc acttctttga actaataaat ttccctcttc atttatgtct
     6481 aaacatttca ctagttctct attatctcct tttaatacat atatattttt atctataatt
     6541 gcagaataat ctctacatat ttttaaggtt ttttctttgg aattatttac agtataatct
     6601 aaatataatt tttcaaattc agtcaataca ttcctaacaa tatctactct ggataacata
     6661 tacccttctt tatacaatga tgtggctttg tcagctattt cttgttcaaa atccattatt
     6721 ttaacattta ttccaattcc aagaacaaca taatttgttc tttcaatttc agcagataac
     6781 tctgttaata taccacatat tttttttcca tttaaaataa catcatttgg ccattttatt
     6841 aatgtatttg ctcctagatt gtttaaagct ttcgttacac ttgctcctgc tattaaagtt
     6901 ataaatggag ctttgaatgg ttccatattt ggttttaata taatactcat ccagattcct
     6961 tcatgaggtt ttgaatccca tcttcttccc aatcgtcctt tccccatgtc ttgatgttca
     7021 cttaaaatca cagtcccatc tacactattt atagctatcc ttttagcatg ttcatttgtt
     7081 gaatctatag tgtcaagatg ttcaatattt tttccaataa actctgtatc taatttatag
     7141 taaatattct gtgggcttaa taaatcagtt ggattttccg ctaatcgata ccctttttta
     7201 tttacagatt ctatattata tccctcttct tttaaggctt tcatatgttt ccatatagct
     7261 gcacgtgaca cccctaattg ttttgataat tcttctcctg aaataaattc agatttatta
     7321 tttaatataa tctctattat tttatctcgc atatttcacc tctaatatga cataatttca
     7381 tgacttattt tcttctaata aataattata cattattaat atacttttaa tactattaat
     7441 tttaaatttc ctttttaatt attataatgg ttgttttata ttttatattg atgatttata
     7501 taattttaca ttttatttaa ataattttaa aataataaaa aaactgaaag tatcaattaa
     7561 attaatacat gcaaatataa tttgcttatt aatttaatat tatactttca gttttagtat
     7621 ttaatccaca tcagataata atcttaaaat gaccaaggtc ttaaaactat ttatctttat
     7681 cagatttatc ttctaaattt ttatttatat caataacatt gtttctttct tcttcatctt
     7741 tttctaattt tatagttaat tgttcttcaa cttctttaga agaattttcc tctgtaactg
     7801 cattttcaat ttcattcaga ggtaactctt tattaaatgc ttttacaaat tgttctgcat
     7861 ctaaagtttc atatataagt aaagcttgtg ctacatactc taatctatcc atattttctt
     7921 gtaatatact tcttgtctta tcatatgcac catcaactat acgctttgtt tcttggtcta
     7981 tctcaaaagc aacttcttca gaataatttc tcttacttga aaaactatta cctaggaata
     8041 cttcatcatc actgtcaaat gacattggac caagcttaga actcattcca tactttgtta
     8101 ccatacttct tgcagtagct gtaactcttt ctaagtcatt tgaagcacct gtagatacat
     8161 ccttaagtac tagctcttct gctactctac caccaagtaa aactacaata ttttctttca
     8221 tttcattttt tgtagcatag aacttatctt caacaggaag ttgcattgta aaccctccag
     8281 ctcttcctct tggaactatt gtaacttgat gaacaggact tacttcttca agtacatggg
     8341 cacaaactgc atgccctcct tcatggtatg ctgtaagtct tctttctttc tcacttatta
     8401 ctttcgattt tttagccaca cctgctataa cctttgttat agcttcttct atagtttcca
     8461 tcttaatttt cttttctctt tttctagctg ttaatattgc agcctcattc attaagttct
     8521 ctatatcagc aggagtaaat cctggtgttc ttctagccaa gacatccatt tttacatcat
     8581 cacttaatgg tttatttctt gagtgaactt taaatatagc ctctctacct tttacatctg
     8641 gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct aagtaaagct gggtcaagta
     8701 tatctggtct atttgtagct gccatgatta ttataccttg atttacccca aatccatcca
     8761 tttcaactag aagttgatta agagtttgct ctctttcatc atgacctcca ccaagacctg
     8821 cacctctttt tctaccaact gcatcaattt catctatgaa tatgatagct ggagcacttt
     8881 tcttagcctg ttcaaataaa tctcttactc ttgaagcacc aaccccaaca aacatctcaa
     8941 cgaaatcaga accacttata ctgaaaaagg gaactcctgc ttctcctgca actgctcttg
     9001 atagatatgt tttaccagtt ccaggaggtc ctaccataag cattcctttt ggtattcttg
     9061 cacccaattc aatatatttc ttaggattct ttaagaaatc tactacctcc tgcaaatctt
     9121 ctttttcctc atctagtcca gctacatctt taaatgtaac tctagttttt tcatcatctt
     9181 tatgaacctt agcttttgat ttgccaaaac tcataacctt tccgcctcca ccttgagact
     9241 gattcataaa cacgaaccaa ataatcacca tgaagaatat taacagtaat gtaggcaaca
     9301 tttcgacaaa ccaaggtgta gatggcttag cttcaccacc aaaagttagt ttcccagcct
     9361 tagcttggtc taacacctca tctgcaagtt tatttcccat cacttctgtt ggaacatatg
     9421 acttaaactt tgtatttgta tcttttatag taccttctac agaagtttga tttacaaagt
     9481 ataatcttga tatattctca tctgttaatt ctctatatac ttcagagaat tttaaatctt
     9541 ttattttttc tgtaggctta cctgagaatt gaactatacc tactataatt ataaaaacaa
     9601 gtagataaaa acctgcccct tttagcaact tattcaatat agaagccctc ctttcataca
     9661 ttgctttgcc ctataatttg ttaaaattaa tttttaatac ttcttttgtg ttttcatcaa
     9721 ttttataatt ttcactgatt ttataatctc caacacatat tattccttca ctatctgtga
     9781 ttataggaat tttacatctt tcttctcttg ggatttttaa atctatgaat aaatctttca
     9841 cttttttact tcccatagca agctttatct tatcaccttg ccttctgctt cttattacta
     9901 tacccccttt taccttatta aaatcaaacc ctttagatga attgtctaat ttcatactct
     9961 tatacctatc tatgctcatt acttgggtct caataatttg ttttaattct tttattttaa
    10021 taaacccatt actaggtaca ttgtaataaa attcaatttc ctcattaaca atttcctcat
    10081 tcgttaatat tatacagtct ttttttctat aaacaaatat atttcttgga agagttaaca
    10141 ttttatttac ttttgaatta tcttctaaag acattatatc ctctatatgt ctttggtcaa
    10201 caaaattagt atctcctagt atatgtttta tggaatttct aagaactcta actttgatag
    10261 catcatgtaa gcaaacaaaa ttgtctaaat ttatctctac aaagcctttt tcttttatat
    10321 tcgaaacttc tctaaattta gcttctgcct ctttctcaat ataatcgtta tcacttttta
    10381 aactattgct catcctcact atagattcta ttacatttga attaaaatta tccttcatat
    10441 atggtaaaag gtctaacctt atcttatttc tagtgtatat attttctaaa tttgttttat
    10501 ctatcctcgg atttaaatta taagcttcac aatactcctc tatctcattt ctttccacgt
    10561 ctaatatggg tctaattatg caattatctc taatataatc aattcccttt aaacctttta
    10621 aacctgttcc tctcatgata cgcatcataa ccgtttcggc ttggtcattg agattatgac
    10681 ctattgctat tttattggct ttaatcttat ccttaatttc ataaaacatt tcatatctta
    10741 gcttccttgc tccttcttct aatgataatc cctcattttc acaatatttt ggtacattaa
    10801 tagactttac aaagaatata attcccatat cctcacaaag tttagataca tataatgcat
    10861 ctttttgagc ttctatccct cttatttgat ggtttaaatg tgctgcatat atttctatat
    10921 taaaatcttt ttttaatcta tttagtacat gcaataaaca aactgaatca ggtcctccag
    10981 aaagacctaa aactatttta tcgccttttt gtattaaatt atgtttattt atagtactta
    11041 gtaccttatc aaatatcata tttatccctc ttacacttag ttttattcac ttttaagtat
    11101 actttctata aaaaaacgct aaacccttgt atcaatttta agaaattaaa gtttaatata
    11161 aatataataa taccaaataa attaaataaa aggtagtttt ttcactacct ttataaaatt
    11221 tctggaaaat agccatagtt attatttagt acctatgtat atatctattt tattcctacc
    11281 taaaaataaa tcttgtacct ttatattata gtctattttc aattttatgt gttccccttc
    11341 ttgtatattt atatctaatt ctcttgtatc aatatcaatt aataaagtac cttgtggggt
    11401 cacatacttt gtagtactac tttctccaca tttaaacagc attgttgagt taatagcacc
    11461 aaactttttg atactaactt gatttttaag tatctttata gtattagtta tttctatatt
    11521 ttcttctttt tccttgtaaa taacatatat tccatcattt ttataaaaaa ttttaccaaa
    11581 ggcagtcatc tctatggtgt ccatatttcc tttttcatca tattgtctag tatttatact
    11641 tatttttaca ctcaacgatt tttcctccat agattcttca tataaactag tatttttgta
    11701 tttctacttt ttgaatctta tcaatttttt tctttaagaa ctctcttgct atatctgaaa
    11761 atttttcagg tatatctgat acataatatt gataagttcc atgaatttct gtattgttta
    11821 taatattttg cacctctaaa atctttttta aatcctttgc agtctcttta gctggattaa
    11881 caagttttat atgctctcca acttcttcac ctattgttct ctttaaaata ggataatgag
    11941 tacatcctaa cactaaagaa tctatatttt tttctttcaa ttcttgtaaa tatatctttg
    12001 ctgtcaattt agccacttct gtatttgccc atccttcttc aacaataggt acaaataaag
    12061 gacatgcttt attaacaatt tctatactct catctatttt agatatttcc aagttgtatg
    12121 cttttgaact tatagttcct tcagttccta ttattcctac aattttgttt tttgtagaac
    12181 ttacagctgt tctagctcct gcttctataa ctccaattat cggtatatca tatttttctt
    12241 gagcttcttt taagcttctt gcagtagcag tgttacatgc tataactata gcttttacac
    12301 cttttgtttt taaaaagttt atagcttgaa aagtatattt aattatagtt tctttggatc
    12361 ttgaaccata tggtattctg gcagtatctc caaagtatat tatatcctca tttggtaata
    12421 tcttcattat ctcttttaga actgttaatc cacctaatcc cgaatcaaaa actcctattg
    12481 gtttattgtt catctttata caacacctac tttacatttt tgtgtttaac aacaacttat
    12541 ttatcgctaa aatttatatt tatattcata attaatatat tattacctaa atgattatta
    12601 tagtttattt tacacaaaaa ataaaggagc tatcttaaat tagaaaataa tttctacaaa
    12661 aagacaactc ctttcactac ttaaaaattt aaatttattt tgcaaacaca tgatttccta
    12721 ttctcatata aggattcaat ctatttatcc atttgcaagt ggctatttct ggattccaaa
    12781 aatatactgc tttattagtt gggtcagttc cataaagagc ttcctgagct gctttacgtg
    12841 ctgagtctgt tggtgccatc tttattgaac catcaagcac aactgagaaa gcattttttt
    12901 gatatatgac ttcttctatt gtgtctggaa atctagggtc caatactcta tttattacta
    12961 cagctgctac agcaacctgt ccctcatagc tttctcctct agcttctcct gcaaccaact
    13021 tagataataa catcaaatca ttatcagata aatttattac ttctttcttt gctatatttt
    13081 tttcttgttt gtttactttc ttaatatttt ctgttggttc atatgcaaat acctctttaa
    13141 taggcataaa tataaaagtc aaagctaata gtgtagccat cagtttactc cacctgtcca
    13201 tgattaaaac cactccttac attcttattt ttaaaatttt ttctctaaat ccttgactac
    13261 ttcaagtact ttaaacttaa tttttactct acttgttttt tttggtgtat cttgttttat
    13321 taacttatat tctttatcag ttaaaacttc cttcaactta tcatctgtag atttatctat
    13381 cactccatta gattcatcaa caaaacatag tggagggaac ataacacacc accagttttt
    13441 tccttcacct ttcccaatga taatctttaa tgctttatat tcaccagctg gaagaactat
    13501 attagaatat tgtttagtag ggaaattgct atattgtata cctaccttta cactgtaatt
    13561 atatccattt ttaagaataa tatttttact tatttcttcc agactactat attccttttt
    13621 tataatagct tcactttctt ttatactttt tgaattttga agctttggct gtaaatattt
    13681 tattacttca tctctaactt ttagtttaag ttcttggtct tcatctgtat tgctgtttgc
    13741 tataacatga aatcttatta atttatcttt ataatctttt gatatattgt ctactttttt
    13801 tacctcacca tttattacta ttgtcattat tgatatgact gatataagac ttaatattaa
    13861 tagtcccaat ctaatcttaa tttttctcat tgaaaattcc ccctcaagat aaatttatat
    13921 catcaattat gtccaattaa atttaattta ttcataatat tttttaatac taataaaaaa
    13981 agctatctta aaatgattac aatcatttta aaatagcttt ttaaacctta tattttataa
    14041 gtctttacta atcatagcct tatctatcat aaactgctct gctttttcta tatgctctgt
    14101 agcatacttc ttggatagct cactatcacc tttaattata gcatcaagta aatatttatg
    14161 ctcttcaaca atattatgtg atgtatcata agcagacata taggtaactc taaatctata
    14221 tacttgttcc cacaatgagt ttattaattg gattaatttt ttattattag ttgctttaaa
    14281 tatacattca tgaaattcta catcaagttt taataacgca tctacatcgg attccaaagt
    14341 gcttttttta aattcttctg atatatcttt aagcttttta atatcttcat cactaattct
    14401 ttcagcggca agatatgctg ctaagccttc taggcttgct cttacttcta atacatccat
    14461 aatatccttt aatgacatat ttgccacata cgcacctttt ctaggcaaca taacgactaa
    14521 accttctaat tcaagctttc ttattgcctc tctcactgga gtcctactta ctcctaattg
    14581 ttctgctagt tgcacttcca ttagcctttg accaggcttt aattttcctt ctaagattgc
    14641 ttctcttaaa ttctcaaaga ctacatctct taatggcttg taattatcca agttcagttt
    14701 agttaaattc tccacaaatt tcaacccctt tttgactgct attaacaaca tacacctgtt
    14761 tatatttttt taataattct ttttttccaa ttagagcatc ttctttattt ttaaatagtc
    14821 caaaaactgt aggaccactt ccactcatca tagaacctaa agcattattt ttcatcatta
    14881 cttgttttat atcacttatt tctttgtgtt tacctatagt aacattttca agaatattta
    14941 ccatgctttc tgatacagct tttatatctt cagattttaa acactctatc aaatatttat
    15001 tatttggtct ttttttaata ttattcaaat ctaatccttg ataaacttct ttagtagaaa
    15061 cgaataagtc tggtttgcat atcaatatat ttgtatcaca tggtaaacct ttgatgtttg
    15121 ttagcttttc tccaatacct tgagctaaag caggtcttcc tgatatacaa aaaggaacat
    15181 ctgcgcccaa attaagtcct atttctttta attcatcttc agataatcta agttcccata
    15241 ggtgatttaa acctactaat actgcggctg cattagaact tcctccagcc atccctgctg
    15301 ctacaggaat atttttttct atgaatattt ctacaccttt ttttatataa aatttatttt
    15361 taagtatttt ggcagcttta taaacaatat tatcttcatc taaaggaata tctaaactag
    15421 tgctttttac ctttatttca tcttcgtcta attcttttat ctttactata tcgtataaat
    15481 ctatcgtttg cattatcatt tcaacaaaat gataaccgtc ttgccttttt cccaagacat
    15541 caatggataa atttattttt gctctactct ttagtcttat aaaatccatt ctatcctcct
    15601 tgtttaaaga ataatgcatc actaacaata atattatact acaaaaaata aaataatagg
    15661 gtattataat accctattat acttaattac tttaaatttt ttaatattaa tctactaata
    15721 cgactttttt ttctaaaata agacacttac caggcttaat tggttcatca agttttatat
    15781 cattaagttc tgcaatttca ttttctgttg tattgtattt ttttgctata ttccagaaag
    15841 tatctccttc tttgcatata tatacgatta tgctaggagc tttagataaa tcatatacac
    15901 cttggtcttc tcccttgata ataaaatttt ctgctttctt atctaatgct tcagtaaatc
    15961 tcttaatttt gataatcagg tctatctggt ccctattcaa atcaacttcg actttatcaa
    16021 tacatgctgt attaaacaca gatgctgtat cagttagatt atccatttct atatcatgtt
    16081 caaatggaat ttcctcacta attttataaa ccaattttaa accttcaact gggacaaata
    16141 aaatttcaac ttttatgatt ccttgtataa tgcttttatt tccttcaata taactatttt
    16201 ctatagacat ggttggacaa acgcttacaa tatcttttat ttgtatatca tcattatcat
    16261 ttcttatgcc ttctctaacc ataaatgttt cttcactacg tcttaaagtc ttatttaatt
    16321 gaataggttt gtggtcaaat tttattatct tttgaggcga atatgcatct tgtaatactt
    16381 ctcttgtaac ttcatctgta acttttacct tacaacaagc catacaatct atttctaaaa
    16441 gacctgtgtt gctttcacta ttttgcttga aaatatgatt aaaatctgac atagacaaca
    16501 atacttcttc agtcatacca tcactaactc cagggacttc tacaaattga gtaaactcaa
    16561 tacctactct atccaactct acaagttcac cctcatatgt acaagctaac ggatttattt
    16621 ctaatactcc accaataata actttattgt cagtcactct actttctttt actttaacac
    16681 atggatttaa acttattata gattgaattt cctctgtgtt tattgttata gtatctctta
    16741 tagaactttc tgctttttct attccaacta tatcttgaaa acaaatttcc tttctgtgct
    16801 tttgtatgcc ttccacttgc gccacatctt ttacaatgtc taacctttgc ttttcaaaca
    16861 aacttcccct tatattcata agagctccta ctttaatctt tctctcattc attatagtac
    16921 aatccatatg ctctacttct gaaaataaca tgtattccat atcttgaata acattgtctt
    16981 tttctattac ttcgtttata tctacttttc cgtctacatt agaaatagta tttttatcat
    17041 ctgcaatata gattacatta taattaaaac tacctctgca aagtatcttc ccatctgcca
    17101 tttcaatttt tttaagtgat atgtatcctt ctgtttttac aatttcatat acatctaatt
    17161 ttttgtctgg tacaactgcc tccgcttcaa tgaatgtttg aaattttcca aagtctattc
    17221 tattgtcaac tttaattaca tctttaatta attccatagt ctacctcctt aagtattata
    17281 aaaatatcac actatattta tatttaagga gcaaaaaaaa aattacacct atttttaaat
    17341 aggtataatt ttaacttatt tgtaatttat cttgatctct aaaaacttgc aactttacat
    17401 tagatgttaa taaatctgag taactataag atactctagg atatccatta ctttcatcat
    17461 ctaactttat aacaaacacg cttggataaa ctttttctag tataccctct tttgtaataa
    17521 tctgctttct tcctttatta gctttcaaca atattttctt tcctatatgt ctctctaagc
    17581 ttactcttat cttatctaga gtttgaacag tagccacaat atcaccttct ttaacgttta
    17641 tatgtttaca taatatcaca tataaacatt tttgtcaaat gtttaaacta attattttat
    17701 atattttttg caaaaatgtc aatactattt ttgaagatat acagtataat tatgttaatt
    17761 tttgctaatg taatattgtt tttggcattg cccatctaaa ttttcctctt gtttcagaac
    17821 caccaattcc cttaacacct gtaatagtct gctctataac ctcatcaata ggaactacta
    17881 catcaattct tgaacaagca attttctcca ctataaaaac agggtctaat gcatgtatca
    17941 ttattcttcc aggcgaactt gcatagtttg ctcctgcact tattattttt tcataatttg
    18001 attggcatgc tcctgcgaat attacaagat tatccaaatt tggttgccat ttacgtgctt
    18061 cttttactgt tttaacaaaa tttgaagagt ttctatagtt actcatatct tctatatctc
    18121 ctctttttat tgtcattgca tcatgacctg ttattactag tatatccgga ttatattttt
    18181 ctagtagtgc tcttacttct ttatactgat tttgctctga aacagctaca cctactgcag
    18241 gtattccaag ttgagcatat acatccaagc aaatttttaa atattcttta tctccatcta
    18301 tctgtagtac ttttccaggc attccatatg tgttaggatt taattgaagt tttggaactg
    18361 ctctagtcat tcttttttgt ctttctttag cttttcttac tgacttatat aataaatttt
    18421 ctacattttt atctattaat atatctttta tatcaggtgc ttttactaat tctaggtcat
    18481 caatataagc atctgctatt actctaaatg caattccctt tagtatagct attttctcat
    18541 tattttcatc tacaccaaaa gagactattt taaatactat atctttgtta tgtgactttc
    18601 tagctacaat gtctcctacc ttcatagtta aacctccaat aaatttttat cttagtttat
    18661 actatgttcg atataaaact ttgtttccta atgtcaaaaa aacatttctt ataatacttc
    18721 ttttatcact cttaaagcat ttttatagta tatcttttct atatcatctt cactaaaccc
    18781 ttcttttttt agaggttcat acagtttacc catctgagat atatcttcaa tctcaacttt
    18841 tgaatctatt ccatcaaaat ctgaccctag agatacaaca tctattcctc caacatttac
    18901 aatatgtttt atgtgtctaa ccatatcttc aagtttactt tcacttttat cacttaaaaa
    18961 taagtggaag aagtttatac ctgtaactcc acctttattt gctaatactt taatcatatc
    19021 atcagttaaa tttctagaat gattcatcat tgctcttgaa ttagaatgtg tagcaattat
    19081 tggtttagaa gaaatctttg caatttcata aaatccacca tctgaaatat gagaaacatc
    19141 aaccaacatt cccaattcat tcatcttatg taccacttct cttccaaaat cagtaagccc
    19201 tttttctcta tattctactt tattatgagg aaagctcaat tcattcacat gattccaact
    19261 tattgtcatc attctaaccc ctaagtcgta aaatttcttt aaattttcta atttaccttc
    19321 aagtactgca ccttcctcaa tagataaaag agctgtaagt ttaccttctg attggttttt
    19381 cataatttct tcatagttag tagctaaagc tatttcatca ctattttttt tcatttcctc
    19441 atgaaattta tttgccatac tcatgcaata atcaaaagga tgttttactt cttcagtatc
    19501 aacaaaaaga gcaaatgttt gagctaatga atcccccttt ttcaatctgt ctatatcaac
    19561 agaatattta ttagacttaa gttcacttgt ttctacattt tccattaatt ttgctatagt
    19621 atcacaatgt aaatcaagaa atttcatatt tataccccct gtatatttta tatttatatc
    19681 taaaaataga aatagttgtc cttatagaca actattgaca actatttttt aatattcttt
    19741 ttaatattac ttaatcttat tcttctttta agttaggtat tactttttta acttcttcaa
    19801 tttgatgcca tgttttatct atattatttt cactaatata tttttttaaa tcagttaaag
    19861 atgtaaaatc tttaactgat aaattgtcat catcatctgc aaatcttaaa ataaacatga
    19921 gactccctta ctttccacaa catttcttat atttttttcc gcttccacat gggcatgaat
    19981 catttcttcc tatttttgct tctttaacta ttgttttaga cttcttataa gcctttatta
    20041 tttcttttct ttggtctgct ggtagtatat cttcccaacc ttgtaaatta tatagccaat
    20101 gagcctcaac agttaccata ttgttatata atttttccca atctattttt atagaaactt
    20161 ctttatcagc ttctacttct tctaaaacta tatcttcttt taagctttca cttattccat
    20221 ctatgaatcc catgaaaaat tcattagtag ttccaaattt ttcagccaat tcacttactt
    20281 taccttcaac aacatccatt ttattattta atatttcatt gtatattcca gcttcaactt
    20341 tcaaatattc ttcccagaat tttacttctg cttcttgact ttcatgatta tcacttaaat
    20401 ttctccattc agtatataaa ctcatacttt ttcctcctaa gtatacctat atttttatag
    20461 tttttatagt ataaaatctt ttagttagat tggaagtttc ttctttttga ttataccata
    20521 ttctatttaa agttaactct tatattatat cataattaaa caattctttt aatactttta
    20581 tagcatactc ttggtttata aaataagggc ttgtcgcaat tgttattact ttggcttttt
    20641 taattaagtt ttttatcttg taaaatttga gttcaaatgg tatatagtcc atatcacttg
    20701 aaaatatatc caaatctata tcaagaacaa actctgattc tacttctaaa tcaaatccat
    20761 atagactatc tattattatt aattctgaaa atatgttata ttttaaagca ggttttataa
    20821 aacttcctac atttaaaact tcatttgtat atctaaatac atcttttaga ttatttacat
    20881 ctacatcata gttctcaggt tctctagtat ccttatgttg gtctacatgc actaacttgc
    20941 atccttttgt aaactcaccc tttaacaaag attttgccca aaaataaaat gcatggttat
    21001 gattgtcaaa cacatatatc tttttatttc ccaggacata ttcgaccata ttctccaatc
    21061 cttttgcttt tatttcctta ttttcatcta cttcattaaa tactacctca ttgcctaacc
    21121 ttacatcatt taatgtacca tttattagtt ttggaacgta tatgctctta ttttctcttt
    21181 tatcatatga aaatacatta tttcctactg gtttttctat ataaaatcca ctatattcat
    21241 aattttccat aaaactttat ttcctccatt gttatataaa tatataagtt ataattttaa
    21301 aaattaaaat gcacttattg taaatcatat catctattat agaaaacatc ttatataact
    21361 aaatataata tcttctctca atatgtatta tacaataagt gcaaaatata tttatataaa
    21421 atatactaca ttcctataac aaaaccatca gcttcaattg gaaggtaact aactcttcta
    21481 ttcaactcca cctttccaaa aaatactacc caatatggtc taaaaatact ttcaatttta
    21541 actatttcaa tctctgggat tcctcccata aacctatgag ttaatctcat agccatttta
    21601 tttgcctttg cttttactct tcgttcgtcc ttatcactat actgcacaat attacaatcc
    21661 acatttttta caatttcata tgtaggaaaa tcttgtacta tactaccaga acctgtagaa
    21721 ccattcacta aaatttttaa ttcatttata tcattagaat atctcctatt cttgaaaatg
    21781 ggtttcgttt tgtgaatttt aagtgtgata attttaaact ctatataatg aagtctaact
    21841 tcatgagcat atttgttctt taatattagc ttactaaata aagaattttt tccactttta
    21901 gctatctcta tagcttcttc ttttgtaatt cttataggta acgctcttat atccataaaa
    21961 tcattctctt tcttattttt ttcttgtcat ttttaaaact ctatatatat aatcaaaaaa
    22021 tattacaatt acaactgaca taactccata tggaattatt tttccaacta taatatcatt
    22081 tctagtcaat ataccataaa atataaatcc tgttattaaa aagtacaata atactagaaa
    22141 accgtctatt ccaagccatt ttatttctaa atcttttttt accttagaca ctaatttaga
    22201 catagcagtt tctcctttta tagattattt tgtgtattta aattttcacc tattctatta
    22261 ccaatcatcc atgcaataaa tgaaaaaggt atagctatca caagaggctc taactcaaag
    22321 ctctgaactg gaaatatttt aaaataacca aaagtcactc caccaacaat cattcctaaa
    22381 ataccaccca tagatgttct tcttccattt ccaagaagcc cacctactat aggtacaaat
    22441 gccgaaccca tccaaatact tcctacaaat atacatgcat catatacatt ttcaaatcta
    22501 aatgctacgc taagccccat aactgctgct aaacatacac ctattctagt aaactttact
    22561 aattgtttct gagataactc tttttttgta attcttccat atatatcatt tgctaatgta
    22621 gccccagctg ctagataata gctatccaat gttgatatta tagaacctaa aattcctatt
    22681 ataaatatta ctcttattcc ttgtggtaat gtatctaaaa ctaaagttat atatccagca
    22741 gctggagcca tttctggata agtagctctt actataagtc ctgtcatagt taacaccatg
    22801 tcaaaacata tccatagtga aaaacaggtt aataaggctc ttcttccaac cttaggagaa
    22861 tctgctgctg aaaatctttg ataaaaagtt gggtctgaat aagctccaaa agaatataca
    22921 attaacatca atgctttcat agctgacata ccttttgtag ggtgcataag ctgtggtgca
    22981 tctttgagtg cttctgtaag accttgaaac cctcctattt gttcaaattg acctggcata
    23041 accatagtta aaccaaatac catacaaaaa aacaatacca tatctgtaat ggcaactccc
    23101 ataagcccac caagtactgt aagtacaacg actactccta ccattattaa tcctattaat
    23161 tctttatcta ttttccatgc tacgttacct atagttccca tagcagttat ttctccaagt
    23221 tgtgcacaat atataaacat caaaattgcc ccaattatag caacatgctt gccataaaaa
    23281 ctttccagta aatcaggaac tgttatattg gttcttatat gaatccttgg accaacccac
    23341 aatgctaaag gcattctccc aatatgtgca gctattgacc aaactgccca tgcagatatt
    23401 ccatatatag ccgcatactc tacactacct actgtaccaa ctcctccata ccatgatgca
    23461 accaaagttc ctactattaa ggaccatggt aaacttcgat ttcctaggta ataactttcc
    23521 atatctttta catttctaga aaaataataa cctattccta ataatataaa tgcaaatata
    23581 actataatcc cattttccca aaatgttaac atatctaatc tccacattct tatattttaa
    23641 tttttaactt taatatctaa atatgaacaa ctctacccat ttcca
//
LOCUS       DAENCV010000054        23587 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAENCV010000054 DAENCV010000000
VERSION     DAENCV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.054"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..292)
                     /locus_tag="KQD82_002156"
     CDS             complement(<1..292)
                     /locus_tag="KQD82_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG4380626.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSK"
     gene            complement(302..1231)
                     /locus_tag="KQD82_002157"
     CDS             complement(302..1231)
                     /locus_tag="KQD82_002157"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG4380627.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(1246..1686)
                     /gene="def"
                     /locus_tag="KQD82_002158"
     CDS             complement(1246..1686)
                     /gene="def"
                     /locus_tag="KQD82_002158"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG4380628.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEDLIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(1704..4229)
                     /gene="priA"
                     /locus_tag="KQD82_002159"
     CDS             complement(1704..4229)
                     /gene="priA"
                     /locus_tag="KQD82_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG4380629.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(4468..5667)
                     /gene="coaBC"
                     /locus_tag="KQD82_002160"
     CDS             complement(4468..5667)
                     /gene="coaBC"
                     /locus_tag="KQD82_002160"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG4380630.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(5672..5938)
                     /locus_tag="KQD82_002161"
     CDS             complement(5672..5938)
                     /locus_tag="KQD82_002161"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG4380631.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(5941..6558)
                     /gene="gmk"
                     /locus_tag="KQD82_002162"
     CDS             complement(5941..6558)
                     /gene="gmk"
                     /locus_tag="KQD82_002162"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG4380632.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(6588..7469)
                     /locus_tag="KQD82_002163"
     CDS             complement(6588..7469)
                     /locus_tag="KQD82_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG4380633.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(7473..8303)
                     /locus_tag="KQD82_002164"
     CDS             complement(7473..8303)
                     /locus_tag="KQD82_002164"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG4380634.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(8590..9624)
                     /gene="ltaE"
                     /locus_tag="KQD82_002165"
     CDS             complement(8590..9624)
                     /gene="ltaE"
                     /locus_tag="KQD82_002165"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG4380635.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            9920..11695
                     /locus_tag="KQD82_002166"
     CDS             9920..11695
                     /locus_tag="KQD82_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG4380636.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSRFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(11910..13562)
                     /locus_tag="KQD82_002167"
     CDS             complement(11910..13562)
                     /locus_tag="KQD82_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG4380637.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     EGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEIKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEKKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(13861..15072)
                     /locus_tag="KQD82_002168"
     CDS             complement(13861..15072)
                     /locus_tag="KQD82_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG4380638.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(15357..15890)
                     /gene="pyrR"
                     /locus_tag="KQD82_002169"
     CDS             complement(15357..15890)
                     /gene="pyrR"
                     /locus_tag="KQD82_002169"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG4380639.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(16250..17197)
                     /locus_tag="KQD82_002170"
     CDS             complement(16250..17197)
                     /locus_tag="KQD82_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG4380640.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(17166..17612)
                     /gene="lspA"
                     /locus_tag="KQD82_002171"
     CDS             complement(17166..17612)
                     /gene="lspA"
                     /locus_tag="KQD82_002171"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG4380641.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(17919..18698)
                     /locus_tag="KQD82_002172"
     CDS             complement(17919..18698)
                     /locus_tag="KQD82_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG4380642.1"
                     /translation="MKKIIWGICICLLSIVGVYTYSHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDGGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(18753..19235)
                     /locus_tag="KQD82_002173"
     CDS             complement(18753..19235)
                     /locus_tag="KQD82_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG4380643.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDISLCNDCANKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(19501..20922)
                     /locus_tag="KQD82_002174"
     CDS             complement(19501..20922)
                     /locus_tag="KQD82_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG4380644.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(21068..21838)
                     /locus_tag="KQD82_002175"
     CDS             complement(21068..21838)
                     /locus_tag="KQD82_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380645.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(21843..23045)
                     /locus_tag="KQD82_002176"
     CDS             complement(21843..23045)
                     /locus_tag="KQD82_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG4380646.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         7656 a         4189 c         2355 g         9387 t
ORIGIN      
        1 tttttgacat accaaatgat gatgcaatga aagatataat aggaaataat ccactgacaa
       61 tattaaaatt tattttcatt atatttgagt taacatactt tcctttaatt actctataag
      121 taactgctgt tgaacatagt attataagta ttagtgatat tattataata tttatcatca
      181 aggagaatat ttgagttaaa cttattataa aaaaattaac aactcctatc aaaattccta
      241 atattaaaat caaaatccca acacttgtta aatacttctt tatatctgtc atacttttca
      301 cctaacccaa tactaaacca gtttctatag tattcccttt tatatattca ccaaccaaca
      361 ttctcttttt attcggcatc tgaatttctt caattaaaag tacattatct atagtactta
      421 cttttatccc atctttgctt acatcaatta ttgtacctac atcttttgtg cttttttcat
      481 ctaacacttt agttttccaa actttcatta tgacatcatt ataggtagta taagcacttg
      541 gccatggatt tactcctcta actaaattgt gtatttctct ggcacttttt gaaaaatcta
      601 tgttacctaa tgatttattc attattggcg cataggaaaa ttcttcatga ttttgtactt
      661 ctcttggagc atttccttct tcaattaatc ttaatgtttc ttttaaagtt tctgctccta
      721 tattcatcat tttatcatga agttctcctg ctgttatatt ttcatcaaga tttacttctg
      781 tctttagtat catatctcca gtatctaatc cttcatccat atacatagtt gtaacaccag
      841 tcttttcttc accatttatt attacccaat ttataggtgc cgcacctcta tattttggaa
      901 gtaaagaaac atgaacattt atacatccaa gtttaggaat ctctaatatt tcttttggaa
      961 gtatctgtcc aaaagctaca actactatta catctggatt taaagattta attttatcta
     1021 taaattcttt atctctagcc tttactggtt gatatacagg tatattattt tcaattgcca
     1081 gttcttttac tgggctcata cctagttttt tacctcttcc ttttggttta tctggttgag
     1141 ttacaacacc caatatttca tacttctcat ctattatttt ttgtaaacat ggaactgcta
     1201 tatcaggagt tcccataaat actattttca tcaaatcaac tcctactact tttctatttt
     1261 gtcaacaaat aaaattccat ctaagtggtc tatctcatga caaaatgctc tagctaaaag
     1321 ctcttctccc tctaactcta ttgtttcacc atttctattt aaagctctga cctttacata
     1381 atttggtctt ctaacatttc ccgcttctcc aacaacactt aaacatcctt cctcatctat
     1441 ttgctcccca gatgtttcta ttatttcagg atttataagt tctataagat cttctccaat
     1501 atctattact actactcttt ttaatattcc aacttgaggt gcagccagtc caacaccatc
     1561 tgcatcatac atagtttctg ccatatcatc taataattgt attatctttt catcaatttt
     1621 ttcaaccttt tttgattttt ttcttaatac tggttctcct atttgaacta tttgtcttaa
     1681 agccattata attcctccta aatttataat acactgtttg gattaatatc tatagataca
     1741 ttgatattct tatcaaatat caaatctcgt tttgttatac atatatattt tattataccc
     1801 tttaagagat taatttcaat attttcatct ttaaagagta tttgccatct ataattttga
     1861 tttatttttg atattgaaca tggatttgga cctaatataa atcctaaatc atttataccc
     1921 cttttttcta ataaataaat caatgatgca tgcatatttt ttatattttt tatcactaat
     1981 ctttcatctt caccactaac aactacactt aacatattat taaaaggaga atatccaaat
     2041 gcttttctta tttttatctc atcttcataa aaaccctcat aatcatattc aatagcatgc
     2101 ttaatagcat agtgttcagt gtcataagtc tgaagtacta cttttccctc tttatcagct
     2161 ctccctgctc ttccagagac ttgagttatc aattgaaatg tagtttcaaa acttttaaag
     2221 tctggaaaat ttaatatcat gtctgcagac aaaataccta ctaaagttac attctcaaaa
     2281 tccagccctt tacttaacat ctgtgttcca ataagtacat ctgcttcttt atctttaaat
     2341 ttatttaata tctcatctaa agcacctttt tttgaagttg tatctttatc cattctcaaa
     2401 gtttttatat ctggaaaaat atattttaat tcctcttcaa ttttttgagt tccaactcca
     2461 aatggcttaa cataagtact tttacattca ggacactctt ttggaatttc tttctcatat
     2521 ccacaatagt ggcatcttcc agtattactt ttcttatgat aagttaaaga tatatcacaa
     2581 ttttcacatt gaaacacata tccacacttt ctacaagata caaaatttgc ataacctctt
     2641 ctgtttaaaa atagtattac ttgattattt tcttcaatcg cataacgtat ttctttttgt
     2701 aatttcaaac taaatatact tctatttcct ttatctaact catctttcat gtctaccact
     2761 tcaatgtttg gaagaggctt atcatttgct ctacttttta tatttataag tttgtattct
     2821 ccagattttg ccctataata ctcttcaatt gagggcgttg ctgaacctaa aataagagtt
     2881 atattatttt ttaatgccat aaatctagca acttctatag cattaaattt tggattcttc
     2941 tcagacttat atgatgactc atgacattca tcaattatta ttaagcctaa ggaattaaaa
     3001 ggtgcaaata aagctgacct agctccaatt aacactctta ctttacctgc tttgacagct
     3061 ttgtaaacat catgtttttc tccttcagat agtttactgt ggaacactcc aactaaatct
     3121 ccaaacctac tctttagcct atctattgtt tgaggagtta atgctatctc aggtactaag
     3181 aatatactgt ctaagccttg atttagagca aattctatta tttccatgta aacttcagtt
     3241 tttccacttc cagttactcc atgaattaag tatggttttt tatcatctac aaacatattc
     3301 gactttatct catcaactgc ttcttgttgc tcattgttta attttatact ttttagtacg
     3361 gatttgtaaa tactttttgg ctctctataa taatccttaa attctagggt tattagcttt
     3421 ttcttactta gagaatttat actttgtttt gaagcattta ataaatccaa taaatcattt
     3481 atctctacat tttcattatt tttaagaaaa cttaatatct ctttttgttt acttcccaca
     3541 tttattttat tttgctctat ataatcgtca atcttatcac tttctaaact caaagatata
     3601 taacatactt tcttttcatt tttgtgattt ttatattccc aacataattt tataattcca
     3661 tttttattca ttgtgtacaa agaactattt aagtttggta tatattttaa cttatcaacc
     3721 ttaattttac ctttactatc aatcactttc tctactattt ctctattttt atcacttaaa
     3781 gttgatattt tttccttcaa ttcaatatca tttaatccac ttaaactttc acctaatagg
     3841 actactttat aattatttaa tttgtatccc tttggatata tcaaatttat acattctata
     3901 taagtacaca aatatctatt tttcatccaa taaaccaact ctaaatcttc ttttctaaat
     3961 attggatttt catctaatat atcaataatt tcttttattt ttattttttc atctaagcta
     4021 tctgtaaatt gaaatacaaa ggcttctgtt ggtttattac cttttccaaa tggtactaat
     4081 attctgtgcc cgatttttat tacatcagat aaaaatacag gaatttgata tgtaaataaa
     4141 ttatctgtat atatagtatt acttctaact attacctttg catatttttt cattataaaa
     4201 cctctactta aatttaatat attgttcata ttttaatata ctttacaaga tattaaaatt
     4261 tcagaattta taaacaattt caagttatta atattaactt tattttaata attaactatt
     4321 aattatattt tatactttat gacaaaataa atgctgaaaa ctacaattta actgacattt
     4381 gcgagttttt actcgctaaa attaaattgc agctttcagc cactttagct gtgataaaat
     4441 acacaaatat catatttaaa aaaggtctta tttctcaagc aacgctttaa ctttatctaa
     4501 aataacattg gctacttctt cttttttcat cttaggatac tcagtaatat ttccctctac
     4561 atctattatt tttacaatat tagtatcagc tctaaaacca gcaccttctt tagttaaatc
     4621 atttgccaca ataaaatcta aatttttctt ttttattttt cctttggcat gttctataag
     4681 gtcattagtt tcagctgcaa atccaattag tatcttatct tcttttattt ttcctatttc
     4741 ataagcaatg tcataatttc tacttagttc tattgataaa tcatcattct tctttttaat
     4801 ttttttgtca gaataagttt ttggtttata atctgcaact gctgcacttt gaattactat
     4861 tttattttca cttaaatttg atactactgc ttcatacaaa tcttgtgctg actctatttg
     4921 aacaaatttt ttaagatttt gtggaataga aagatttgta ggacctgata ttaatgttac
     4981 atcagcacct ctagctatag cttctcttgc tatagaatat cccattttac cagaagacct
     5041 attagttaaa tatcttactg ggtctatact ctctacagtt ggaccagctg taactatcat
     5101 actttgacct tttaaatctt gctctttagt aagattagtc aaaacttcct ctactattat
     5161 atctggatta gctagtttac ctgttccaat atctccacat gcaagccttc cactttccgg
     5221 ttcaataaat ttatatccaa agctctttaa agtttgtata tttttttgaa caattggatt
     5281 ttcatacata ttagtattca tagctggtgc tataagaacc tgtgcttttg ttgccataac
     5341 agtagttgtc aacatatcgt cagctatacc atttgcaatc ttgcctataa catttgcact
     5401 tgctggcact attaagaata aatctgcttt ttttgctaat gatatatgtt caacttccca
     5461 agtttttggt tcttcaaaca tatcttctac tacataattt tgactcagcg attgaaatgt
     5521 aagtggttta acaaattctg ttgctgattt tgtcattata acatggatat ttgcatttag
     5581 tttttttaac ttacttacaa catcacaagc cttatatgca gctataccgc cacttatgcc
     5641 aattacaaca gttttatctt ttagcatgac cttactcctc ttctgaaatt tgttggtgtt
     5701 gctcagcatg atgtctagct tcttctatct ctatttcctc ttctgtcaat aatctatatg
     5761 ttatttttcc acttgcaact tctttagttg ctacacatac aggcttttcc ttagttttat
     5821 ttgaaacata tggttcttct ccatcaatta attttcttgc tctctttgaa acagtcccta
     5881 ctaaatagta tctgttgtct attttttcta gtacctcatt gatagatggt tttaacataa
     5941 ttataattcc tccttaaatt tctctattat aatattttta tatctgctaa ctttattctt
     6001 ttcagatgat attatacctt ctatttcttt agcagatgtt tttacatctt cattaaatat
     6061 aaaataatca taatccttaa tcattttaat ttcttcatat gcacagctaa atctcttctc
     6121 tatttcagct tctgtttctg tacctcttcc aacaattcta ttttttaatt cttcaagtga
     6181 tggtggtagt acaaatataa atactccctc tgggcaaact tcttttattt gttttgctcc
     6241 ttgcatttca atctccagta atacatcttg tcctttttct aaacattcca ttattgccgc
     6301 ttttggagtt ccataaaaat tatcataaat ttgagcatac tccaaaaact cacctttttc
     6361 tatcatctct tcaaactttt ccttacttat gaagaaataa tttactccat caacttctcc
     6421 agttctgggt tttcttgtag tagctgatac agaaagtttt attgtatcat tttctttaag
     6481 tagttctttg caaatagtac cttttcctgc acctgatggt cctgatacaa ctagcaataa
     6541 tccttttttc acaaacattt aagcattccc cttctaatat tgtattctta ttctatattt
     6601 tgtatttgtt ctcttatttt ttccaattcg ctttttactt ctactactaa attagttata
     6661 tttatatcag atgacttcga acctatagtg ttagtttctc tattcatctc ttgtattaag
     6721 aaatctattt ttctacctat agagtcgtct ttaaatatag tatttttaag ttgccctata
     6781 tgacttttaa atctaactat ttcttctgtt atactgcttt tatctgcata tattgcaact
     6841 tcttgtgcta atctattctc atctattata cttgggtcat ccagtaattc acttattctc
     6901 aaattcaatt tttctctata atcttctaca acactacttg agtatttttc aatctcttct
     6961 atatgatttt ttaataaatc acatctcatg gcaatatctt cagctaattt tttaccttct
     7021 tcacttctca tttctcttaa tttgattaat gcatcttcaa ctgcttgatt caacattgac
     7081 cacaataaat cttcatcttc ttccttttct tctattttca caatatcagg aaactttgca
     7141 acattcataa cgctaatatc atctataata tcaaatttat tttttatttc ttttagtatg
     7201 tctatatact gagatgctaa tccctcatca aaatttaagt ttacatcttc tttgcctaat
     7261 aagtcaaact ttatgtataa atctactctt cctcttttta cataattttt tactaaattt
     7321 ctaactttat cctctaaaaa tgaaattttt cttggaagac gtatgtttat atctgaatac
     7381 ttatgattta tcgttttaca ctctactaaa aagtaataat tatcatcctt gtattctcct
     7441 cttccaaaac cagtcatact tatagccatt aattaaacct ccaaaatacc ttcacatatt
     7501 tttactgctg gacctgtcat ataagagaaa tctcctacat ctattttaac tgttccacct
     7561 tgagacgtta catttacact actttctacc atattaagta aattgcacac tattgctgat
     7621 gcagtcatac cagtaccaca acctaaagta tatccacaac ctctttccca agtataaaca
     7681 tgtatattat acttatcttc tacctttaca aaatttacat tcgttttttc aggaaatatt
     7741 ttattatttt caatctcttt tccatattta caaacatcat ctacatctaa ttcatctact
     7801 ataactactg tatgaggaac ccccatcaac atagaagtaa ctttaagctc cctatctaaa
     7861 acctttatac tttcttgcac aaaacgttct ttatttgtaa gtacaggaac gtccttagct
     7921 ataaaacttc cttttcccat attgactctt atagagttta tttctctatt ggtaagattt
     7981 atttttatat cttttatacc atctaatgta tctactgtaa aactttcttt tttaactata
     8041 caattatcat aaacaaattt acaaaaacat ctaaggccat ttccacacat agctgctctt
     8101 gaaccatcag aattataata taccatttct acatctgcaa cttttgaatc tttcacaact
     8161 aacaatccat ccgctcctac agaaaatctt ctatggcata ctttttttgc taaagcagta
     8221 taatcagatg gatttatctg gtcgaatctt ccatctattg ctataaaatc atttcctatt
     8281 ccatgtagtt tccaaaattt cataagtatc gctccttcgt aaattaacgc ttaaactatt
     8341 ataacataaa aaaattccca taaacttata atttatggga aaagttttgt aatgacaact
     8401 ataatttgag gatgtgaggg agagttgcca ttacaattag ggtaattata ttatgttttt
     8461 aagctagttc tttctatgtt tatatattaa tatttctata tgaattttat gtatgtttta
     8521 tttaaactgt ctatgaattt tttaattaaa catctggtaa ctagctataa tccttctagt
     8581 agatatctat taacatacac ctattaattc tttaagtgtt tttaaaacat agtctaaatc
     8641 ttccttagta atatctacat gagttacaaa tctatattca ccattttcca ttttattaat
     8701 ttttatattt ctttcaaaaa attcttttat taaaacatcc tcttttataa tatcttcact
     8761 catcttaaaa aataccatac ttatatctgg attaacattg tttacttcaa tgccatcaat
     8821 tttatttaat tcacaagcta aatattttgc attttcatgg tcttcattca atcttaaagt
     8881 cattttttca atggcaatta taccagctgc tgcaactata cctacttgtc tcattccccc
     8941 acccataact ttttgctttc ttctagcttt tgctataaat tctttacttc cagcaagtat
     9001 agaacccatt ggtgcagcta aaccttttga cacacagaac ataacactat cacaacactt
     9061 tgctatttct ttagcttcta cacccaaagt taatgctgcg ttaaataatc ttgctccatc
     9121 taaatgtact ggtaagttat gtttctcagc tattttttta atttctctta aattatcaag
     9181 actcacaact gttccacctg aatatgcatt ttctacacat ataagccctg tctgtggttc
     9241 atgtatgtca tctcttctaa ttgccttttc aacaagttca ggatttagag ctccactttc
     9301 gtcttcatct atagttctaa gctgaactcc agctattaca gcaggtgccc caacctcatg
     9361 agtcacaatg tgataacctt taccaataat tatttcttgc cctcttaggc aatgagtaaa
     9421 tagagctaat tgattcccaa aagttccact tggaaggaaa atagcatctt cttttccaac
     9481 tttagctgct gctaattctt caagtttatt tacagtaggg tcatctctaa agattgaatc
     9541 tccaacagga gcctttgcca tagcttctct catttcgtca gtaggcatag taacagtatc
     9601 acttcttaaa tcgataaatc tcattttttc aaccttcttt catatttata tactatagaa
     9661 ccttaaaatt gattttttct gtaaagttca tatactttca tagcttatac tctacacaat
     9721 aattatacta tatttacttt atatttaatc tactttaaat tattaatttt aaaaataaag
     9781 tttcctctct aataaatata aataattata caatttaaat attaatctct attgtttaaa
     9841 ttccccctag tcacaaagcg tatattatgt gtataattaa tttactgact cattttggtc
     9901 aaagaaaggg gtgctataaa tggcattaga tggattagtt atacattcaa tagtcgatga
     9961 attatcatca aaacttacag gtggaaaaat agataagata catcaacctg aagatgatga
    10021 ggtaattttt aatataagaa ataataaaga aaattttaga ttggttttaa gtgcaagtgc
    10081 ctcaaatcct agagtttatc ttacaagtaa ttatcaaaaa gaaaaccctc taaaagcgcc
    10141 tatgttttgt atgttactta gaaaatacat acaaggtgga aatatagtag aaatttctca
    10201 aataggtttt gaaagaataa taaaaataag cgttgaatct ttagatgaat taaaagaaaa
    10261 gacagttaaa aatattatga ttgaaattat gggaagacat agcaacataa taataactca
    10321 tggtgaagaa aataaaatta tagattctat aaaaagagtc ccttttagca taagtagagt
    10381 tcgtcaagtt ttacctggtc atgattattc attaccacca gaacaaaaca aactcaaccc
    10441 tcttgatgat atatctaagg atttgtttat taaaaaccta gaggaattgg aaggacctat
    10501 ctttaagtct atatattcta gatttctagg tataagccct attatagcta aagaaatttg
    10561 ctacagagca ggtgtaaatc aaaatgctat cataaaggat atatctgatg aacaatttga
    10621 ttctttacac aaagtatttt gtaatttatt caatgatata aattctaaca aatactctcc
    10681 ttgcattatt atagataaaa aggtggataa ggtagttgat ttcagttgta taaaccttac
    10741 attatttagc gatttatcat atataaataa ggatagtatg tctcgtattc tggaagattt
    10801 ctatagaact aaggatatta aagatagaat taatcaacgt tcatctgatt taaagaagag
    10861 tatatctgtt aagcttgata gactttataa taaacttaaa aaacaagaag aagaattatc
    10921 agaatccgaa aatgcagaca tttataagat aaaaggagaa ttgattactt catatattta
    10981 tatggtagaa aaaggtatgg aaagtattga agttgctaac ttctatgatg aaaattgtaa
    11041 tgatgtaaca atagaattaa ataaaaactt gacaccatct gaaaatgctc aaaaatactt
    11101 taaaaagtat aacaaaatga aacatgctaa agtcgagata tctcatcaaa tttccttaaa
    11161 taaggaagaa atagattact tagaaaacat aattttaagt attgaaaact gtgaaaattt
    11221 agctgaatta caagatataa aagaagaact tgctaaagtt ggatacatca agactcaaaa
    11281 gaaaaacagt aaaaaggata ctatcccatc tacaaaacct catgaatttg tgtcttctga
    11341 tggatttaaa atattggttg gtaaaaacaa taagcaaaat gactatctta cactaagact
    11401 tgctgataat gatgacttat ggatgcatac aaagaatatt cctggttctc atgtaataat
    11461 aaagtgtgct ggtaaggaag tccctgataa tactgtattt gaaggagcta tgctagcagc
    11521 attctttagt aagtcaaaac tttcttctca ggtgcctgta gactatacta agagaaaaaa
    11581 tgtgaaaaaa cctagtggtt ctaaaccagg tatggttatt tatgaaacta acagtactat
    11641 ttatgttact cctgaagaag aaacagtagc caagcttaag gttaaatctg aataaattct
    11701 gcactaaata caaaataaat gttagacaac tacacattat cattctaaat aaaaatagtg
    11761 gccttcttat aaagtacaat aatagggcca ctttctaatt agcttacttt taattttcta
    11821 acaaaatact tttctgtttt aataactata cttatattcc caacaaatat tttcagtttt
    11881 cttatataga tgtaattaaa aagttaacac taaaagctat tttttaattt agtgatttgt
    11941 tttagtaatt cattatattc tgattcatac aattcttttt ttagattatc tttttctata
    12001 gataacatag atataacagt tgtaagtcta ttttctaata ctagaagatt ttcttttttg
    12061 atttggtttt ctctactagg agttttttta tttctgctaa ttatatattc gtcataatta
    12121 caatcaaact cccttatttt agcatctttt atctctataa tatagttaca aatatgtgat
    12181 acaaatactc tatcatgaga cactataagc atagttttat cagtatttat cagtgccttt
    12241 tccagtgctt ccatagattt tatatctaag taatttgttg gttcatcaaa tactaaaaaa
    12301 ttattatctt ccaatattat tttacatagt gctattttta ccttttctcc accactaagt
    12361 acttttactt ttttgtagac atcatcacct ttaaacccaa ataggcttaa gtttatcctt
    12421 ataaaagatt catcaaatga actattaact ttagtattgt atagaacact ctttttctca
    12481 tctaataaac tttgattttg gtcaaaataa cctactttta ccttgttatt tatttttata
    12541 ttgtcatttt tatcagccaa tatttctttt atcagagtac tttttccaca cccattatca
    12601 cctaataatg ctatcttttt acctcttttt atcttaaagg aaacattatc taacaggagc
    12661 ttattttcta actttagagt catatctttt atctctacta gatttttgct aattatctcc
    12721 ataccatctt gaatcttaat gttcatttct ttagaaactt taggtttatt ctttacctca
    12781 agtttatcaa ttctgctttt tatagccttc acatttgagt ccaacttttt cttattagtc
    12841 tgaccaccca ttttgtgcaa cctagcttct gaattaccca ttctttttgg tgtttttctt
    12901 atgccctcac ttaaagcttt tttatataaa agagcttttt caagcctttt tttctcttta
    12961 acatattctt gatactcaaa atcagctctt tttacttctt catctttttg cattaaataa
    13021 tcagtgtaat tgcctttgta agcttttagt tttccatctt ctagttctaa tatagttgta
    13081 cataactcat ccagaaaacg tctatcatga gatattaata ataatgctcc ctcatgcctt
    13141 ttaaacatat cttccaaaac cccaatgctt tttttatcta agtttgaagt tggctcatca
    13201 gctattatga tatttttctt attactcagc gcatctgcta tttttaactt aaccttttcc
    13261 cctcctgata agtgttcttc aaatttatct ggagcattta aaagactctt ctccttacta
    13321 tgaccacatg tttcaatatt attttcactc tgacttatat aagaatagct ttctgtaagg
    13381 taagtatatc cttcatcaat ttctatttct cccaatattg cttttaataa tgttgttttt
    13441 ccttgaccat ttgaaccaac tagacctatt ttgtcatttt ctaatatttc aagtttatct
    13501 atatccaaaa ttaatttatc tgcataatat ttctttaaat tctctacttt tactaacaac
    13561 ataaaaaatc cctccttaac acacttaaat ataaggagag attctataaa taggcataac
    13621 aaataaacgt atagactata atctactctc cttaatttaa gcattgcaaa taaagccacc
    13681 aaaataggct tttcaaaaat tacaatactt taaattataa aaaatagatt atattctcat
    13741 tgtcctagtt accttgccct ttctttcatt tatttttaat cattatatca acattaatta
    13801 caaatgtcta tagaaaatta actaatttta gttattcaat atagaactta ttctttaaat
    13861 ttattcttct tcaactttaa agtcctgatt atttaaaatt ctgtttaata caattccaac
    13921 aattgcagct aaactaagac cagttatctt tactgtactt gttataggga ttccaacaga
    13981 aataccttta ttagataaat atgttgttcc aagacctatt aataatataa ctgctatgat
    14041 taatatattt attttacttt ctacacaaga actgtccctt atagttttta atcctacata
    14101 agttatcatc gaaaatagca ttatactaat tccacccata actgaacctg gtatactttg
    14161 aagaaatcca ccaaacttgc ctacacatgc aagcaatata gcaaatattg ctgtacgtct
    14221 aagtattgaa gggtcatagt ttttagttat agcaagtact gctgtatttt ctccataagt
    14281 tgtattggct ggtcctccta aacaccctgc aactatagtc gcaaatccat ccccaagtaa
    14341 tgttctattt aatccgggat tttctataaa gtttttacct actacagcac cattagtagt
    14401 catatctcct atatgttcca taaatacagc taatacaact ggagatataa tcacaattgc
    14461 tcctaaactg aacttaggaa ctgtaaagtt aggtattgca aataaacttg cttctgtaat
    14521 agtagttata tctactaatc ttaatatcaa acagattata tacccagaaa aaactgctat
    14581 taatattcct aattgtttta taaatcctct accaaactta ttggttaaaa ttgctattgc
    14641 taaagttata aatgctatta caaaattggc tgaagccata ctaaaagctg ttggcaataa
    14701 atttaatcct ataactacta tcatagctcc tgtaacttgt gcaggaaaat ataatttaat
    14761 tttatttaca ccaactactt ttactataat cgacattata atataaatta atcctgctac
    14821 tattatacca ccttgagcat atgctaaatc tccaccatac gcctctttag ctgctagtat
    14881 aactggtata aacgcaaaac ttgaacctaa aaaggcaggt acttttcctt ctgtacaaaa
    14941 atgaaatatc aatgtaccaa caccagcaca gaaaattgca actgatggat taaaacctgt
    15001 caaaatggga actaatactg tcgctccaaa catggctaat agatgttgta aagacattat
    15061 cgtttttttc ataaaaacct ccaaaattat tatatataaa taaaccctct gtttaattaa
    15121 tatcttttaa ttaattatca ttccctagat tctatttttt aacctctata tacttattat
    15181 ataatttatc attattttac attttacgac atcaaaaact caaataaagg agaataaaaa
    15241 ctacattaag actcacatcc ataaaattat aatatttatt ttgtgcaatc tatgtaagct
    15301 ttatcctcct ccctgactat tcaatgctaa gctttttata taatacttaa aaataattac
    15361 tctttgattg taactgaatc ttctccatca atttctaata aactgacaga tataatttca
    15421 tgtctagaag taggtacatt ttttcctaca taatctgctc ttataggcaa ctctctatgc
    15481 cctctgtcca ctagcactgc taattgtatt gatttaggtc ttcctatatc tataatagca
    15541 tctagtgaag ctctcacagt tcttccagtg tataaaacat catcaactaa aactactgtc
    15601 ttatcattta cattaaaatc taaataagtt cctttaacta caggttcaac atgaattttt
    15661 tccaaatcat ctctatacaa agttatatca atatctcctg tatctacttt tgttccttct
    15721 atttgttcta ttttcttaga aattctattt gcaattggca cacctctagt ctttattcca
    15781 actaaaacta gattttctac acctttattt ctttcaatta tttcatgact tattctagta
    15841 attgctcttg ctattgcctt ttcatccatt aattgagctt tttctaccat atcataatta
    15901 ttgtcaaaga tataatatat ctatttgaca aatctactcc tttcttttaa ttttttataa
    15961 atttattttt atttaaaatt tcaatttaaa caacaaaaaa acttctcatc acaagacaag
    16021 aagctcttat ataaaaaata tacagtgcaa tttagcaatt gtcttacgat atctctgtat
    16081 cgtttaaaga taatttttta atctatcaaa tcaaattata tatttatatt tcaaattttt
    16141 caagcttttt taaacaaaat actttaatat aagatatttt atttaaattt cactaaattt
    16201 atttacttat ataaaaacaa aaaataattt ataagtatta tacaaatggt tattttatct
    16261 ttttaataac ttccttaaaa tactcaggta attctgaatt aaattcgata tattccttag
    16321 tagtaggatg tataaatccc aacttcttag cgtgcaaagt ttgtcctttt aatttaaatt
    16381 tagtttcttt tggaccatat acataatcac ctaaaagtgg gtgatttata gacaaagcat
    16441 gaactcttat ttgatgagtt ctaccagttt caagcctagc tctcatatgt gtaaacttat
    16501 caaatctttc tacaacttca aagtgagtga ttgcattttt tccatctttt actacagcca
    16561 tttttaatct atccttagga tttctcccta taggtttatc aacagttatt ttatcttctt
    16621 taaccacacc atagcatata aattcatact ccctagttat agtatgttcc tttaactgtt
    16681 ctgctagaaa attatgtgca taattgttct tagctatcat aagaagacca gaagtatctt
    16741 tgtctattct atgaactata ccaggtctaa taacgccatt tattgatgac aacttatctt
    16801 tacaatgata taaaatcccg tttactaacg tattttcata gtttccagga gcaggatgga
    16861 caaccatatc ctgaggttta tttattatta agacatcatc atcttcatat acaatatcta
    16921 ttggaatatc ttgaggtaaa acttctaata ctttaggttc tggtatctct ataacaatat
    16981 tatcttcctc tttaaccaga tatctagctt tttctacttt attattaacc tttacttttc
    17041 catcttttat aattttttgt atataacttc ttgacatatc acctaattgt tctgctagat
    17101 aaatatcaag tctaacttct tcttcctctt caagtactaa aaactctttt acttcatcca
    17161 ttatatcacc tacttttact ttcaaaaaat aaaacatata tacataaaaa cacagttcct
    17221 acaactacaa atacatcagc tatattgaat acatattccc atataattct aaagtcgaag
    17281 taatctacta caaaacctaa tcgtactcta tcaattagat ttcccaatgc accagatata
    17341 attaatataa ttcccaacct tccaaatata tgtactttcc ttgtattaag atagtatagt
    17401 ccaaatactg ttgcaagtaa tgcaacaatt ataaatatcc attgattatt ttgtaataat
    17461 ccaaatgctg cacctctatt ttcgacataa gttaaatgaa atacattatt tattattggt
    17521 attgttgata catccaccaa attattcaat acccatattt tagacagttg gtctaaacct
    17581 atgagtagaa ttattattaa tatatatagc aatgattttt ccccctataa attatttaaa
    17641 aaccaatttt atttgaagca cactattatt atacaaaaaa aatgctctaa agagcaactt
    17701 tattaaaatt gtattttctt aataaagcta gaacaaaatt taaaatagta ctactttttt
    17761 tatattgtag atttctacat atttaaaatc atatcattat gtataaaaaa tttaaaaata
    17821 ccatactaat caatattttt tgtgtatttt atagattaaa gattaatgat gtctgtaatt
    17881 taaacttaca atcaccacta atcttcaaac aaatattttt acttttgcac ttgcttattt
    17941 ttatctaaat gttgtataag ttcagatact gttacgaatt tatatcctct ctcttttaat
    18001 ttaggaatta ctacttctaa agcttgtata gtttgtgatt tatcatttct tttagtgtta
    18061 taatcatgta aaagtattat attaccattt tggacctttg taaggatagt atttataatt
    18121 gcatctactc cagggttaga ccaatctcta gggtctaagt ttgaccaaag aattatattc
    18181 atgcctttag aaactataat atcacagaca cttttactca tggctctata tggaggtcta
    18241 aatatctttg gttctactcc tattacactt tttataatat tttgcgtatc tgttatttct
    18301 ttttcaacac tactataact attttttgca acatttatat gtgtaaatgt atgattccct
    18361 atttcatgcc ccccatcata ttgtcttttt aataattctt tgttatattc tactttttct
    18421 ccaactacaa aaaaagttgc ttttacatca tttttcttta atatatcaag tacttgtggt
    18481 gtaaaatcct catctggacc atcatcaaaa gtcaatgcta aaattttttc atcctcattt
    18541 ccttttttta ttattacatc ttcataatcg tcttttatat tgtcatttaa actatatgct
    18601 tcttttgaac tatcttgatt atgtgtatta tgactatatg tatatacacc tactatagat
    18661 aataaacata tacatatacc ccatattatt ttcttcatgt cattacctct tttctaattc
    18721 attctagcaa tatattttat aatgtcagtt ttttaatctc tcaatattcc atttttattt
    18781 aaatcagtta aatttgttag gtcttctgaa taaaagttag aatcttctac atcagggttt
    18841 tgcattgcat cacctaataa attattttct ttatttgcac aatcattaca taaacttata
    18901 tctggcaata tatccaatct ttctaaatct atttgattat gacataaatc acaaattcca
    18961 tatgttccat tctctatttt agataatgct aagtctattt gatacaatct tccctcttca
    19021 tgatttatta gactgttttg catattttgc atatataaat ctgttcccat gtctcctata
    19081 tgattatcat aactagataa ttctcctgat gaatactttt cacttgtatg tttagttgta
    19141 tctccaaata aagtattatc tttcatatcc tctactaaat tagttaaatt ttctctttct
    19201 ttcaataatt tatctttata ttttttagta tccattttta acacctctaa ttttaatatt
    19261 tacatcataa aaatagtatg tggaaaagta aattaaacat acaataaaaa taagatttga
    19321 ttcgaaaaat agaatgtctc aaaatgaaat ttttagttca tttaaccctc ttttttatat
    19381 aaaatcaaat atatttttat cctttcagca ataaaaaaga gacttatctc tattttgaga
    19441 tagcctcttt tttgaacata atttaaatac aattttttga tttttatagt tattatttta
    19501 ctaacttgcc aatttatgat tattttgttc cttaatatgc tttgcttttt taaagaataa
    19561 aagaccaaga attattgcta caataacacc tattccatta gatatattaa ctggtagtct
    19621 aaatccttct tttgcttgca ttatataact aaatgttact acagtcataa aaagtgctgg
    19681 taatgtagtt atataatggt ttttatttgc tttgactaaa tatgcagaac cagtccacaa
    19741 cattatcatt gcaagtgttt gatttgccca agagaaatat ctccaaatta tattaaaatc
    19801 tataaatgta agagcaacac caactgcgaa taatggtaaa gctattaaaa atctattttt
    19861 gactgcatct tgattataat tcattgcatc tgctattgtc agcctagcac ttctaaatgc
    19921 tgtatctcca gaagttatag gacatgccac aactccaagt atagctaaag ctcctccaat
    19981 aggacccatt aatccaacag atactgtatt tactactgtt gctgctccac catcattaaa
    20041 tatcacatta agttgaggta tgccatggaa atatgacatg gctgctgctg cccaaactaa
    20101 agctattata ccttctgcaa ccattgaccc ataaaatact ggtcttgcct cactctcttt
    20161 ctcaacacat ctcgccataa gtggtgattg agttgcatga aaacctgata ttgctccaca
    20221 tgctattgtt ataaataagt atgggaataa aggtgttcca tttggattca tattttgtaa
    20281 atttatattt ggtatgttat acccctttat tatcaatcct ccagctacac ctactgccat
    20341 aattaataat gctgctccaa ataaaggata tattttacca atcaatttat ctattggtaa
    20401 aaccgtagcc ataatataat aaagtattat cactccaacc catatttgtg cgtttacacc
    20461 tgtcaatgtc tttaataatc ctgctggacc tgttacaaat acaactccaa ccaatactaa
    20521 aagaataaca ctaaatattc tcattgctat ttttgcacca tttcctagat aaagtcctac
    20581 caactctgat gcagatgtac ctttgttttt cattgataga tatcctgaag caaaatcgtg
    20641 tactccacca gcaaagattg ttccaaacac aatccataaa aatgctgatg gtccaaataa
    20701 tgctccttgt atagctccaa atatcggacc taaccctgct atatttagaa actgaattaa
    20761 aaagattctc ttccatttca ttggcacata atctactcca tccctacaag ctatagctgg
    20821 agtttgattt ttgtcattgg ttccaaaaac cttttctaca aaagtaccat agacaaaata
    20881 acctactata agtactacta ttgaacctaa aaagcttacc ataaattcat ccccttagtt
    20941 tttatataat tgctttataa atttttatga gtttaagaaa actatttcca gttcaaatag
    21001 taatactttt ttatgctaat ataaatatta attacattaa gtgcatattt aagtacatga
    21061 aatgacttta tatatgcatt aacagcctaa attcttttac tttgcttcta cttacagtta
    21121 cttcataatc actatttttt aattttaata tgtatgtatt attaaaccat ggtataattt
    21181 cttttacttt ggttaagttt acgatataag acctatgtgt tctaaaaaat gttttatcat
    21241 ttataagatt ttcgacttct gatatgcctt cttttaatat aaacttttct ttctcagtat
    21301 atataaatgt ctgcctttca ttagcttcac agtaatatat atcatcaata tctattacaa
    21361 ctaatttatc acctttccaa aggctaattt tatgtgttat ttcttctatc tcctgattta
    21421 ctgcttcctt cttatatttg tataaattac tatttacatt agatagttca atatcactca
    21481 tctcactttt ttctaattta ttcagcatag aaataattct ctcttcagaa tatggtttca
    21541 atatataatc aaatacatcc aaagaaaaag catctactgc ataactttca tatgctgtta
    21601 taaaaacaat tttaggtctt gatttaaact gatttaatgt ttttgcaagt agcattccat
    21661 caagatgagg tatatttata tctaaaaaaa ttacatctat tttattttcc tgaataaagt
    21721 ctaacacctc tataccattg gtaaattcac ttactacctc gattccactt ttattttcaa
    21781 taaaatatct caattccttt cttgctggaa attcatcttc cactattata gctctcatac
    21841 ttctactcct ttacataaaa tactatcaaa gtcccattat ctattcttct tatattaagc
    21901 cctcttccat acatcagttt aagccttaaa tgtacattat aaagcccaat cttattttct
    21961 ggcatattat cttgatatac attatttata atttcctcac ttatacctat accattatcc
    22021 tctattgata ttctcacagt attttctaat gaatacggta acttttttat ggatattttc
    22081 actacaccag ctctaccact ttctaaaata ccatgtatta ttgcattttc cactaatggt
    22141 tgtataatca aacttggaat ctttatattt acatcttcta tatcataaga tacagttagt
    22201 aattctccaa acctagcttt ttctatctct acaaaagatt taacttgttc aatctcttta
    22261 tttatatcaa ttaagttatc actatattca agattatatc ttagataagt tgataaattt
    22321 attattaact ctctagcctt atctggacta aatcttataa atgaagctat tgtgtttagg
    22381 gaattaaaca aaaaatgtgg attaatttgt ctttgaagtg cttttatttc tgctttattt
    22441 ctcgcttcta tatctttttt atctttctca atcccctcta ctatagatat aacaaatcct
    22501 attccaatct gcccaactat taaaggtaaa tatattccac cgattatttg aattgctaaa
    22561 gaatgtggat atgatattaa gtatataagt aatatagtaa aactttcaga aatcatgcct
    22621 accaatattc catacattac tctatgttgt tttggtatcc tcttatatag aaatcctgat
    22681 aatacacctg ctagtatact tgataaaaga catggaacag atgttatccc cccaatatcc
    22741 ataaaatatc tatgaatccc tgaaaaaact cctgctgtaa tacctaccat cggaccaaat
    22801 aaaatgccac ctgatactat agatattatt ctagtgttta ctattgaccc catataattt
    22861 ataccactat aagttccaaa tatggctaaa aaagtaaaaa ctaatgctat acagaccaag
    22921 tcattaaaac tatattcttc cttttgaaat atctgcttaa atatttttaa ctttgttatt
    22981 agaagaaaca atactacaat aaggcttgtc ttttctatta atgcgattaa aattgatgtt
    23041 ttcataatcc ccctttaaaa aataaatttt tacacactct aaatttatta tggttgttat
    23101 aaattacata tttgaagtaa tcctatcaca aaactttcca catttaatct tttaattaaa
    23161 taagaaatag ttgtacttct attaacaact aatactagct agcataagct tgtaataact
    23221 tgtagttatt attaacttta caattctagt atttaatttt atactttaga aatctctttt
    23281 cttagaaata atactttcga atatcatata atacaatatt atgtagattc ttactcatac
    23341 aacatatttc tttaaaaatt ttatactatt ctttgttcaa atatctcatt tgatttaatt
    23401 gtgttaaaca cacattatca tctctctgtt atatatttat tatatagccg tatatcgata
    23461 aagtaaataa atcccatatt taaaataata tgtttgacaa taaattatta taatgtatat
    23521 ttaccataat aatttgtcaa ccttgaaagt tacttctata attattgtta aataattctt
    23581 ctaaaaa
//
LOCUS       DAENCV010000055        22205 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAENCV010000055 DAENCV010000000
VERSION     DAENCV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.056"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            180..1364
                     /locus_tag="KQD82_002177"
     CDS             180..1364
                     /locus_tag="KQD82_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380647.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1741..2850)
                     /locus_tag="KQD82_002178"
     CDS             complement(1741..2850)
                     /locus_tag="KQD82_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG4380648.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3313..4506
                     /gene="nifV"
                     /locus_tag="KQD82_002179"
     CDS             3313..4506
                     /gene="nifV"
                     /locus_tag="KQD82_002179"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG4380649.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4531..6456
                     /locus_tag="KQD82_002180"
     CDS             4531..6456
                     /locus_tag="KQD82_002180"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG4380650.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6478..7473
                     /locus_tag="KQD82_002181"
     CDS             6478..7473
                     /locus_tag="KQD82_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG4380651.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7799..8272
                     /locus_tag="KQD82_002182"
     CDS             7799..8272
                     /locus_tag="KQD82_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380652.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8306..8800
                     /locus_tag="KQD82_002183"
     CDS             8306..8800
                     /locus_tag="KQD82_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4380653.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8897..9418
                     /locus_tag="KQD82_002184"
     CDS             8897..9418
                     /locus_tag="KQD82_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG4380654.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            9607..9918
                     /locus_tag="KQD82_002185"
     CDS             9607..9918
                     /locus_tag="KQD82_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG4380655.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            9946..10632
                     /locus_tag="KQD82_002186"
     CDS             9946..10632
                     /locus_tag="KQD82_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4380656.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            10864..11748
                     /locus_tag="KQD82_002187"
     CDS             10864..11748
                     /locus_tag="KQD82_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG4380657.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            11847..13022
                     /locus_tag="KQD82_002188"
     CDS             11847..13022
                     /locus_tag="KQD82_002188"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG4380658.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13150..14004
                     /locus_tag="KQD82_002189"
     CDS             13150..14004
                     /locus_tag="KQD82_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380659.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFYEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            14048..15283
                     /locus_tag="KQD82_002190"
     CDS             14048..15283
                     /locus_tag="KQD82_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG4380660.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            15384..16325
                     /locus_tag="KQD82_002191"
     CDS             15384..16325
                     /locus_tag="KQD82_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG4380661.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(16544..16897)
                     /locus_tag="KQD82_002192"
     CDS             complement(16544..16897)
                     /locus_tag="KQD82_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG4380662.1"
                     /translation="MDRKRELKQLYKEMKFDIGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFIFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            17193..18104
                     /locus_tag="KQD82_002193"
     CDS             17193..18104
                     /locus_tag="KQD82_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG4380663.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            18365..18799
                     /locus_tag="KQD82_002194"
     CDS             18365..18799
                     /locus_tag="KQD82_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380664.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            18822..20357
                     /locus_tag="KQD82_002195"
     CDS             18822..20357
                     /locus_tag="KQD82_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380665.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVVLVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDEVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            20441..21808
                     /locus_tag="KQD82_002196"
     CDS             20441..21808
                     /locus_tag="KQD82_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4380666.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            21843..22067
                     /locus_tag="KQD82_002197"
     CDS             21843..22067
                     /locus_tag="KQD82_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG4380667.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         8666 a         2229 c         4062 g         7248 t
ORIGIN      
        1 aatactattt aaaaaataaa ataaatatat aaaaaatatt taatataaag gtggaaataa
       61 gttgaaaatg taaagtcatt ggaaaaatat agttgaattt aatgagcaaa aatgttattg
      121 ttttatcaat aaagtgttaa aataaaaact ttcaatatca ttttagggag gatgatttca
      181 tgcaaaaaag tgtaaagaaa gcaaaagtaa cgggctctat gttagctatt tttggtgttg
      241 caagcgttct ttttagttca catgcaggag gaggatttgc tacagggaat caagaaactc
      301 agtactatgt gcagtatgga tggacagcac cacttatggc aattttagca atgattatac
      361 ttactgctac catgagagaa gttatcataa tgtataataa taacaattgt aggaattata
      421 aggatttatt ttgtgagctt tggagaccat atccaaaact tgaaataata tgggagatat
      481 attactatct aatggtttta atagctgtaa gtgctgttat agcaggtgca gcagcagtat
      541 ttcaaagtat aggagtaaat tattttgtag ctgtatttat tattggagtt gtacttcttg
      601 tctttactat atttggtgct atgcttgtat ctaaagcagc tacagcaatg acaatagcta
      661 ttctagtatg tactcttact atttttattg ttggtataaa agctaaagta ccagaaataa
      721 ctgaaatatt atcaaataga acgagtttca cacctggcta tatcaagcca atacttaata
      781 cttttattta tgcaggtttt caatcggttg ttatacctac acttgcagga tgttcaagac
      841 cacttcttaa gaactcaaaa gaagctacta aggcaatgat aatttcattt gttatgaatg
      901 caattgcatt aggacttgct gtaactatgc taatgggatg gtatagagaa attattgctg
      961 ctgggcaaac aacactccct actctttatg ttgcaggtca atctggaaat catactatat
     1021 acataattta taatgttgca ttattccttt gtttaatgtc tacaggagta actactatat
     1081 ttggtcttgt aaatagattt gaagaccata aagcactttc tttcctttct tcaagaatga
     1141 aaagaagggt atttacagct tgtgcaataa tggtagtgtc aatgcttata tcacttacag
     1201 gattaagtaa tattgttaaa tatggatatg gatattgtgg ttatcttggt ctattcacta
     1261 tagtaattcc attccttaca ctaggacatt ataagaataa gaagtttgca aaggaaaatc
     1321 cagaagctaa atggccagcg gaattaaatg aaaatgtaaa ttagatgtag tagcaaaaaa
     1381 tattgctaaa gagaatacta tagataagaa tactatagat aatatatctg catgtttatt
     1441 aagattattt gcaaaatcta atattaaaca actataaaca agcaatataa aactaagaat
     1501 aaaagataaa aagagggtgt ctcaaaatgg acttttagtt tataaggcac tcttttttat
     1561 atgaagtcaa atatattttc attattttga caataaaaga gactaatcct actttggata
     1621 gcctcttttt tctttaatat aaaatagtat aacagcaaag tatagattga tacatgtttt
     1681 ctgtagcttt ctatatatta cagtgtttgt gtggcattgg tattttaagc aatctataaa
     1741 ttataattct ataattcttc tactaatttt agattctata gccgtctcta tttcacttaa
     1801 cattcgttca tttttagggt caataaaagc acatgtatag ccttcttcac cagctctacc
     1861 agttctacca atacggtgga tgtagtcttc tggtgtttct ggtaaatcgt aattatatat
     1921 atgacttact ccagatatat caagaccacg agaagctaca tctgtagcta ttaagtattg
     1981 tatatctaaa tttctaaatg atttcattat tctttctcgt tttgattgag caatatcact
     2041 atgaagtttt tgacaattat atcctcttat agcaagagct tcttcaagat tgtcaaccct
     2101 tctcttagtt ctacaaaata ttattgccat aaaaggatta tcttcatcta gtaccttaca
     2161 taaggcatct aatttttttc tatctgtagt ttctactact tcttgtttta tattgttaag
     2221 agtgatttct tctttttgaa tagaaactac aacaggattt tttgtgtatc tataagctaa
     2281 tttttttact tgagaattta tagttgcaga aaagcatagt gtttgctttt tgtttgaagt
     2341 ctcttttaaa atagcttcca cttcattttt aaatcccata agcaacattt ggtctgcttc
     2401 atctaaaaca aaagtcttca acttactaag gtctattgtt tttctattta gatggtctaa
     2461 aagcctacct ggtgtagcta taatcatatg aatatttcct tttagtttct ttatttgaga
     2521 accaatatct tttcctccat atgctgcaag aatacttaca tctttagatt ctttaagttt
     2581 catagcttct tctgttattt gaatagctag ttctcttgta ggagaaagta taagtacttg
     2641 tatatcattt atatctaatg atatattttc aaatatcgga agtaaaaaag ctagtgtttt
     2701 tcctgttcca gtttgagctt ctgctattac atcttttcct tcttttatta gtttaatact
     2761 ttctttttgt ataggtgtag gtgttgttat tcctgatttt tttagaatat ttattgtatt
     2821 ttcactaatc cctaagcttt tgaaatccat tgttatctta cctttcatga aaaattttct
     2881 tttttattaa ccaattaata atatcatatt tataagtatt tttagtaaat tttttaatta
     2941 ttatttatat agcttaaaaa tatattaaga tactttaaaa tggaatgtaa cttaaattaa
     3001 attttagtag gaattttata ggtttatttt taaaggtttt ttgaaaataa atctatttaa
     3061 aaattaaatt aaaatgcaag aaaaacataa taactttcat ttttaagaag ttttcacaaa
     3121 aaagaaaaaa tatagtgcaa taaatgatac aaagtaatat aatagattac aagatatgaa
     3181 aagctaaaaa aataaattgc aaatgaaatt aaaatgaata aaaaataaaa aaataatcat
     3241 aagttagagg tatcaatgaa aaataagact taatgttttt aaaattaaaa gtatactaag
     3301 gggggcatta atatgtgcgt tatatcaaaa gatagagcaa aggaaataaa aatagtagat
     3361 actactttaa gagatggaga gcaaacagct ggggttgtat ttgcaaatag agaaaaaata
     3421 atgattgctg aaatgttaag tgacctaggt gttgaccaaa ttgaagttgg tatacctact
     3481 atgggtggag atgaaaaaaa tgtaataaag cacatatgta gtagaaattt aaaatcagat
     3541 ataatggctt ggaatagagc agtaataaaa gatgttgaag aatctatttc ttgtggtgtt
     3601 gatgcagtag ctatatcaat atcagtatct gacattcata tagaaaataa attgagaact
     3661 tctagaggct gggttttaga aaatatggct aaaacagttg aatttgcaaa gaaaaatgga
     3721 ttatatgttt cagtaaatgg agaagatgct tcaagagcag atatagactt cttaactgaa
     3781 tttataaatg ttggaaaaca agcaggggca gatagattta gatactgtga tacagtagga
     3841 gttatgaatc ctttttctat aaagaatgca atagaaactc tttatgaaag aactaatttt
     3901 gatatagaaa tgcatacaca taatgacttt gggatggcaa ctgcaaatgc acttgcagga
     3961 atagcagcag gagcaaatta tgttggtgtt acagtaaatg gattaggtga aagagcagga
     4021 aatgcagcat tagaagaagt tttaatggca cttaagtgtg tttataaatg tgatttaaat
     4081 aatatagata ctagaaaatt tagaggaata tgtgagtatg tagcacaggc atcaggaaga
     4141 atacttccta catggaaacc agtagttggg gataatatgt ttatacatga atctggtata
     4201 catgcagatg gagctttaaa agacccacat aactatgaac catttgaccc aagtgaagta
     4261 aatcttgaaa gaaaaatagt aataggtaaa cattcaggaa gagctgcagt tgtaaataag
     4321 ttgagtgaat atgaaatgta tatttctcca gaaaacgcta ccaagctatt aaatgctata
     4381 agagctacat ctataagatt aaaaagaagt ttaatggata aagaaattct tcaattatat
     4441 tgtgatatac tagcacatga gaaaggcaca acagaagaag aagcagttag aggttcatat
     4501 atttaagata taattgttta ggaggaaaat atgggagata atatagttta taaaataatt
     4561 aaaaaacata tagtagatgg agaagctgtt gctggaagtt caattggtat aaaaatagac
     4621 caaactttaa cacaagattc tactggaaca atgacatatc ttcaactaga agctatggga
     4681 atagataaag taaagactaa aagaagtgtg gcatttgtag accataatat gttacaacaa
     4741 ggatttgaaa atgcagacga ccataagtat atacaaacag tggcagataa atatggagtt
     4801 tatttctcta agccaggaaa tggtatttgt catcaagtat ttttagaaag attttcaaca
     4861 ccaggagata cacttttagg ttctgatagc cacacaccta ctgctggagg tgtgggcatg
     4921 atggctattg gagctggtgg attagatgtt gctttagcca tggcaggagg agcatactat
     4981 ataaaagcac caaaagtctg taaggtgaat ctagttggaa aattaaataa tatggtatca
     5041 tcaaaagata taatattaga agtgttaaga aaacaaactg taaaaggtgg agtaggtaaa
     5101 gtttatgaat atggtggaga aggtgtcaaa agcctatctg ttccacaaag agctacaata
     5161 acaaatatgg gggctgaact tggagcaact acttcaatct tcccaagtga tgaaaaaaca
     5221 ctagaatttt ttaagagtca aggaagagaa gaggcatgga tagagctaaa accagatgca
     5281 gatgcagttt atgatgaaga gattactata aacttggatg aattaaaacc attagctgca
     5341 aaacctcata gcccagacaa tgtagatgaa gttgaaaata taggcaagat taaaatagac
     5401 caagtggcaa taggaagttg tacaaattct tcttatgaag acttaatgaa agtagcacaa
     5461 atcctgaaag gaaataaagt acataaggat gtgagtttgg taatagcacc aggttcaaga
     5521 caagttatgg agatgattgc tagaaatggg gctttggcag atataataag tgctggagct
     5581 agaatactag aaaattcttg tggtccatgt attggtatgg gtcaatctcc aggtactgac
     5641 tctgtgtctt taagaacttt caatagaaat ttctatggta gaagtgggac tttgtcagca
     5701 caagtatatt tagtaagtcc agaagtggca gcagtaagtg caatcaaggg agtgcttaca
     5761 gacccaagag aatttgatat taaatttaca aatttagatg taaatgaatt tttgatagat
     5821 gatagcatga taattaaacc tgctgatgta ggaagtgatg tagaagtagt tagagggcct
     5881 aatataaaac ctttccctct taatacagag ctaagtcagt ctataggtgg aaaagttata
     5941 ttaaagacag aagataatat aactactgac catataatgc catcaaatgc taagttactt
     6001 ccttttagaa gtaatatacc gtatcttgca aactattgtt tcaatactgt agatacagaa
     6061 tttcctcaga gagcaaaaga caataatggt ggatttatag ttggtggaga taattatggt
     6121 caaggttcaa gtagagaaca tgcagcttta gcaccattat atctaggtgt taaaggggta
     6181 atagttaaga gctttgcaag aatccacaag gctaacttga taaatagtgg tataattcca
     6241 atggagtttt gtgatgagaa agattatgaa aatatatctc ttttagataa tctggagata
     6301 ccaaatattt tagacaatct aggtagtgga attttagaag ttaagaatac aactaaagga
     6361 acttctttta aggtcaaagt agaattgtca gcaaaagaag ttgatgtatt aaaagctggt
     6421 ggaaaattaa actacactaa aaatcaagct aattaatatg aaaagaggga atattgaatg
     6481 tataaagtga cacttatacc aggagatgga ataggaccag aagttgcgaa agctatgaaa
     6541 aaggtagtag aagctactgg agtagaaata gaatgggaag aagttaatgc aggagaggca
     6601 gttattgaag agtatggtac tccacttcct gaatatatta tagatagtat aaaaaagaac
     6661 aaaatagcta taaaaggccc aattactact cctgtaggta aaggatttag gagtgtaaat
     6721 gtaactttaa gacaggcatt agatttgtat gtcaatttaa gacctattaa aagttttaag
     6781 gggataaaat caagatatga agatgttgat ttagtggttg taagagaaaa tacagaagat
     6841 ttatatgctg gaatagaaca taaaataggt gattatgcag ctgaaagtat aaagataata
     6901 acaagaagtg caagtgaaag aattgttgat tttgcatgta actatgttaa agataataag
     6961 agaaaaaaag ttacagctat acataaggca aatataatga aaatgtctga tggattgttt
     7021 ttagatgtgt ttagagaagt tgcatcaaaa catggtgtag aatatgatga tttgattgta
     7081 gatgcagcag ctatgaactt agttttaaat ccagaaaatt atgatgtaat ggtaatgcca
     7141 aatctttatg gagatatact atcagattta ggtgctgggc ttgttggagg tttaggtata
     7201 attccaagtg caaatattgg taaagattgt gcgatatttg aagctgttca tggaagtgct
     7261 ccacaaatag caggtcaaaa taaggctaat ccaacagcac ttattcaatc atcagtaatg
     7321 atgcttagat acttaggtga atatgaaaat gcacaaaaaa tagaaacagc tttagaaaaa
     7381 gtatttcttg aaggaagtaa attgactgta gatttgggtg gctcagcttc tactacagag
     7441 tttgcggatg aggtgtgtaa gtatattgta tagattttat ttagggaaaa agtaaaatat
     7501 agattgaagg aaagaggctt tctcaaaatg gagataagcc ttttttattg gaatgataaa
     7561 atatatttga ttttatacaa aagagagttc ctcataaacg aaaaagttaa ttttgggata
     7621 tccattttaa ttttaaaatt aattgattaa tatgcaaaga aagattaaat aaatttatta
     7681 tttttaattg aactttcaac caaaatatgg taaaatttaa aaaagttgaa ctttcaacta
     7741 aaaatgaata tattgattat gtgaaattct ataaaacaga aactatttga ctttagccat
     7801 agtagtttca gtggaggtgt gtttgattaa gactttagac agtaatatat taagagaagt
     7861 aggtacttta tctagagcag taaattcaat aaatgatatt aagtacaagg aattaaagtt
     7921 acaaaaagga caatttacat ttttgacaag aatctgtgaa aatcctggta taaatcttgt
     7981 ggaattatca aatatgttaa aggtagacaa ggctacaaca accaaggcta tacaaaaatt
     8041 aataaaggca ggatatgtag ataaaaagca ggatgagttt gataaaagag gatataattt
     8101 aactccaact gataaatctt tggaagttta tgagttgata attgaagaag agaatagaag
     8161 tatagagata tgttttgata attttacaga tgaagaaaaa caagtggtaa ctaagttatt
     8221 agaaaaaatg agcaaaaacg tagaaaatga atggtttaaa gtaaagagat agtaaataaa
     8281 tacattaact taaggagata tttttatgat tagaaaagcg aatatgaatg atttagaaag
     8341 cattatgaaa attataaaat ctacagtgga agaaatgaag acttataata atacacaatg
     8401 ggatgaaaac tatccattag aaaaagactt tgtttcagat attaagaaac aagatttata
     8461 tatttatgaa gtggatggag aagtagcagg gtttatatgt cttaattatg aagagccaga
     8521 agaatattta ggcttaaatt ggtcatctaa taaaaaagca atggtaatac atagaatggc
     8581 tgtaaatcca aactttagaa agacaggaat agcatctaag ttagttgatt ttgctgaaaa
     8641 attagctgta gaaaacaatg tatcatactt aaagagcgat acatattcaa taaactcaaa
     8701 aatgaattta ttacttacaa agtgtggttt tataaaaatc ggagaaatga gttttttagg
     8761 aaaggagaaa tctttctact gttatgataa aatattataa agagcattta taatacaagg
     8821 ggtaaataat agattttcag cattagtaag ggcataatat aacctagtat atgctattat
     8881 gaaagggatg atttatatga ttggatttct aaagaaaaga agaagtatta gaaaatacaa
     8941 ggatgttgaa gtagaaaaag aaaaattaga taaaatatta aaagctgctc ttttagctcc
     9001 atcatcaaag gggcttagaa catgggaatt tatagtggta gatgataaag aaaagctaat
     9061 taatttatct caatgtagaa ctaaaggtgg tggatttttc ttaaaaaatg cacctttagc
     9121 tattgtaatt attgcagata aagaaaaaaa tgatgtttgg atagaggatg caagtatagc
     9181 tgcttcatac atacaacttc aagctcatga actaggttta ggttcttgtt ggatacaagt
     9241 aagaaataga atgcatgatg ataatataga agctgataag tacattagag aagaattaaa
     9301 agtacctagc aagtacagtg tagaatgtat aatttctata ggatatcctg atgaagaaaa
     9361 gaaagcatac agtgactctg atttagacta taaaaaggtt cattttaaca acttttagta
     9421 tataatatgt tgtattaaga cattatattt ttttctattg ttaatatata cttaaaattg
     9481 taaaaatata tctgaatttt actaacttat ctaggggtta tgtacaagga ttttaaagat
     9541 aaatatagaa attaatgttt ataatcgata cataacttga gtaacaaaat ttaaggaggt
     9601 taaagtatgg gtatatttaa aacaaaaatg gatgaggact ggaaagtaaa ctacattaaa
     9661 gagtttaatg aaatgagaga ttcttatgag agtaaacttc aaaaaaaaca gtttgaggta
     9721 gatagtttga aatcagaatt ggatagacta agaagctata aaaatagcct taagccaaaa
     9781 gaaaaacaaa ttactgatga tgatataaat aatataaaga gtttaagaag agatggctta
     9841 agctataaag aaatttcaaa tcaaacaagt tggagcaaag caactgtaag tagggtatta
     9901 aatggacttt acgattagca tatacatggg gtgatttaaa ttgggatgaa gtatagttac
     9961 aaaaaatgca taatagattc tattttattg atgtttatag ttcaaatatt aaggatgatt
    10021 ttgaattatg tacttctaag tcaatttgaa tttactcttg aaaattttaa tattataaac
    10081 ttaatttctt ttacattagt tggtttatcg ttaattttat ttttaaaaga taattctttg
    10141 tataacaagg ttagaaatag aaaaatcaca gaagcttttg aagaaaataa aaacaatata
    10201 cttattgaaa agtgtaagct gatacttttt gttgtggttt tatctttagc aataattgta
    10261 acttattgta ctaagggata tgtacttttt aatgtaacta tgatgacttt gtctgtattg
    10321 atagttccaa tatttgaaga gttatttttt agagaatata tatggaacta tctaagtaat
    10381 tttataaagt caaagagtaa aattatctgt ataacatcta tactatctgg aatttataat
    10441 ataggataca tagatgttat aagaaattat gtaatattgt ataacaattc gagctacacc
    10501 tttgaagtta ttatttcaaa aattatgata ggtactgttt ttggtattgt acttggatta
    10561 gttaagtata ggtttagaga tgtagggttt tgtatattat tgaggagctt atttgctata
    10621 tttattaggt agatagtata atcggtttat aaggttgttt taaatttaat tttaaatcaa
    10681 ccttatttta tttgtgtttt ataaagcact tatatattaa taactaataa atttaataaa
    10741 aattatttga atatttttaa aatatataaa tatgtattga catataaata ttatgaatta
    10801 caatttatat aaactaataa aaattaaata atattaatca acttattgaa tggaggaata
    10861 aaaatgaaat ttgtgacttt ttgtagtagc aatgaagaaa aaataggtgt atttaattca
    10921 gaaacaaata gtatatatga aattaatagt ttaggattaa gtaaattata tactgatatg
    10981 aatgatttta ttgaaaacgt atctacagga gatttagaaa aaataaaaaa caattcattt
    11041 gaaaacgcaa agtgttataa gttagaagaa gttaaattat gttccccaat tgtaagacct
    11101 aaaaaagata ttatatgttt aggtctaaac tataaagacc atgtcaatga aatcccagat
    11161 ggagttataa aaaacgtagt tatgccagat tatccaattt atttttctaa gagagcagac
    11221 aaaataattg gagtggatga taagataagc ttacatggag atttggtaga gaagttggac
    11281 tatgaatcag aacttgcagt aatcataggt aaagaaggta taaatatatc aaaagaagat
    11341 gcttatgaat atatatttgg atatacaata gtaaatgata taagtgaaag agcacttcaa
    11401 gataaacatg ttcaatggtt tagaggaaaa agcttagaca cacatacatc tatgggacct
    11461 tatatagtac ataaagaaga atttgaacat cctttaaaat tagatataag tagtgtagta
    11521 aatggagaag taagacaaaa ttctaataca gagtacttta tatttgatat acctactata
    11581 ataaatgatt tatcaagagg aatgactcta aagccaggag atataatttc aactggaact
    11641 ccagcagggg ttgctatggg gatgaatcct caagtatatt taaagcatgg cgatatagtt
    11701 gaatgtaaag tcgaaggaat aggagtttta aaaaatatag ttgattaatg gataaatatg
    11761 ccatataatt ttattagtac gaataattta attaatatag gattaatcaa acttatgatt
    11821 aatttattat agagagggat gagtatatgg caagagaact agaattaaag tatggctgta
    11881 atccaaatca aaaaccatca aagatttata tgaagaatgg agaacttcct atagaagtat
    11941 taaatggaaa acctggatat ataaattttt tagatgcatt taatagttgg caacttgtga
    12001 aggaattaaa agaagctaca ggtctcccag cagcaacttc atttaaacat gtaagtccag
    12061 caggagcagc agtaggggta cctttatcag atactttaaa gcaaatatat tttgttgatg
    12121 atttagagct ttctccatta gcttgtgctt atgcaatggc aagaggagca gatagaatgt
    12181 cttcttatgg cgattttata gcactttcag atgtatgtga taaggagact gcaactatta
    12241 tagcaagaga ggtttctgat ggtataatag ctccaggata tacagatgaa gcccttgaaa
    12301 tattaaaagg aaagcgtaaa ggcaactata atattgtaaa aatagatgaa aattatactc
    12361 ctgaacctat tgaaacaaag gatgtttatg gaattacttt tgaacaaggt agaaatgaaa
    12421 tattaataaa tgaagaccta ttaaaagata ttccaactga taataaaatt tttacagata
    12481 gtgcaaaacg tgatttaatt atagctctta ttacattaaa atatacacaa tcaaattctg
    12541 tttgttatgc taaagatgga caagtaatag gggtaggtgc aggtcaacaa tcacgtattc
    12601 attgtactag acttgcaggt aacaaagctg atacttggta tcttcgtcaa catcctaaag
    12661 tattaaactt aaaatttaaa aaggatatag gtcgtccaga tagagataac actattgatg
    12721 tatatctttc tgatgattat atggatgtgc ttgctgatgg catatggcaa aacttttttg
    12781 aagaaaaacc agagcctcta actggagaag gaaaaagagc atggcttaaa actttaacag
    12841 gagtagcact tggttcagat gcatttttcc catttggaga taatatagaa cgtgcaaaaa
    12901 gaagtggggt aagttttata gcacaaccag gtggttctat tcgtgatgat aatgttatat
    12961 taacttgtaa taagtataac atagtcatgg catttactaa aaatagatta ttccatcatt
    13021 aaaatactga aagtataaag ataatataga ttaagtaaaa atctacaaca agttttgttg
    13081 tagatttttt tatttgctgt ataattttaa atagaacttt atttttagat attcaatttg
    13141 gaggttgata tggctttaaa tataagaaac tatagcctat tatgtaatca aggaacagct
    13201 aaatttaatg aagatgtagt cggtataaat ccatttggcg catgggtttt agatggtgct
    13261 acaggactta atggaaaaaa tttagtgtca actgaaagtg acgcaaggtg gtatgttcag
    13321 tggtggaata ggtatttata taaaaatata aataaagaag aatcattaaa gagtattatt
    13381 aatactggaa taatagaagt aaaaaaagag tatgaactaa gattaaatgg gtttaaggtt
    13441 gaaaaattag atttaccatc tagttctata gttgttgtaa aatttcatga aaataaagta
    13501 gagtattttt tattaggaga ttgtgctttg tttattaaaa atggagattc caaagttata
    13561 aaagatagga gtatttgtaa atttgataat actgtatttt atgaaatgag taaactttct
    13621 aacttggaag agatggcatt tgatgagatt agaactagtg ttatggatac aatagtaagc
    13681 aatagattga agaaaaatac aaaagagggc tattggatat tggattttga tgaggaagct
    13741 gtggaaaatt ctatatatgg atacattgaa ataaaaaaag attttcaatt gatgctaacg
    13801 agtgatggtt ttacaagtgc atgtgataga tataatctaa taaaagaaga agaattgata
    13861 cgtatagcag aaaaatttgg tataggatat atacataata aagtaaggga ttttgaagat
    13921 aatgactata aagcggttaa agtgccaaga tttaaagtaa aagatgattc atcttgttta
    13981 tatttagata tatataatga ttagactgta taaaactatt taattttaga aaggaggcaa
    14041 gtgaaaagtg aaaattcttc atataataac acaaaaacca aatagtacag gtagtggtat
    14101 atatttgtct ggtatgataa aagggttcga aaaaatcggt cataagcaag ctgtaattgc
    14161 aggtatagat gtaaatgatg atgtaaattg ttttccaagt gaagtaagtt tttatccagt
    14221 aaagtataat tgtggggagt taaattttcc tgttgtaggt atgagtgatt ctatgccata
    14281 tgaaagtaca agatataaag atttaaatat agacatgata aatagattga aatatcaatt
    14341 taaagtaaat atagataagg ctatgaatga ttttaagcca gaacttataa tatgtcatca
    14401 tttgtacctt ttaactgcat ttgttaggga aatggtaaaa gatataaagg ttatgtctat
    14461 ttgtcatgga acttgtttaa gacagttaaa taccattgat ttagagaaag aatacataat
    14521 agcaaatata aggaaattag atttaatatt tgctcttcat gaaaatcaga aatatgatat
    14581 tataaagact tttggagtaa gtgaaagtaa agtagttgta attggtagtg gttataatga
    14641 tgatatattt tataataaaa attataaaat aaaggatgat aaaataaaaa tagtatttgc
    14701 aggtaagata tgtaaatcaa agggactgat tccatttata aaagctatat ctaaattaaa
    14761 atatagtaaa gatttaatag aagtcaattt tgcaggtact ggaagcgata tcgaatcata
    14821 taatgagata gttaaactgg caagtaagtc tccatttaaa atgaatttcc taggaaagtt
    14881 agaacagaga gatttagctg agctattcaa caggtcacaa atatttgtgt taccatcatt
    14941 ttatgaggga ttaccagttg tagttttaga agctttatct tgtggcacag atgtaatcac
    15001 tactgatatt cttggtgtta aagagtggat tggtagtgag attaataatt ctggaaagat
    15061 agaatatgtt tctctacctt ttatggagaa agaaggtatt ccaaaagatg aagagttata
    15121 tgattttgaa aataatttgt ataatgcaat agattctaag atacaatctt tattgaataa
    15181 ctcaaataag aaaacatctg tagatatgtc aaaaaaaacg tgggatggct tagcatatag
    15241 aataaatgaa gttattttaa tggatgaatt gtgtttagtc taaaattatt taaattataa
    15301 aaaagtgtaa ctatttttac actattttta aatgatttta tattatacta atactaaata
    15361 tatttgtttt agggaggtaa tttatgagga ttagtaaaaa ggtattggcg ttaggtattt
    15421 cagcattact gttaagtgtt agttttccaa catcaataaa cgcactagat aagattgaaa
    15481 atattcaagg agtagataaa tatgaaacag ctgggcttat ttcagataag caagatttta
    15541 caacagcagt tcttatcaat gcagactcta caatggtaga tgggattgct gcaagtggtc
    15601 ttgcaggagt aaataatgca gctattttat taactaataa ggatgatata ccagaagcaa
    15661 cgcttcaaag actgaataga gttactaaga tatatgtaat aggtggagaa aattctataa
    15721 gtaaagatgt agaaaaaatg ttacttatga gaagaatgca agttataaga attgatggtg
    15781 tagatagagt agatacaagt tataaaattg caggcgaaat agaaaaaatt aaaaacagtg
    15841 ataagatgtt tttagtaaat ggatttaagg atgaagctga tgcagtaagt gttgcatctg
    15901 ttgcttatag agatggagct cctataatat tgacaaaaga tataccttca gcagaagaag
    15961 acacagactt agacccatgg tttggagttt ctcctgttcc agtatatgct attggaggag
    16021 agtctacatt aagtgattac atagtgagca gatatagagc aactagaatt ggcggtgttg
    16081 atagatatca aactaataaa aatgttattg aaaagttcta taatggagca aaagagttct
    16141 atataacaag tggagacgat ttagtttatg ctttagttgc atcaccactt gcaaaaaatg
    16201 ctcctgttgt tttagtttca aataaaagtg ataagagtat attaagtgga gctagtaaag
    16261 taacagcaat aggtataagt gataagagta ttatagagca atgtttggat gctgtaaaga
    16321 agtaatggag tgttattagt aaaatgaata tacacattga aaattagtag attgttcatt
    16381 ttataatggt ataaaatata tgggaagagg ctatcaaatg gagataagcc tctttttcat
    16441 tgttaaaatt atgaaaagat actttatttt atataaaaaa gtatcttatg aactaaatgt
    16501 ctattttgag acactatcta cttttcttat attatattat tttttatatg gaataaattt
    16561 cataagtatt atcttccttt aatttttcta accaaaccat ctctaaaata tctagttctt
    16621 ctaaataatc tttctctgtt ttgttatcat catgttttat aagttctaaa acttcaaata
    16681 taaatgcttt ttcaccatat tttttccatt cattatttaa ttcttttatc atacatgagc
    16741 cagcaccaag ctgaaatttg agagagttaa atttgctttt tatgtcatta ctttttccaa
    16801 ggtaaatctt tttagtaata tcatttttta ttatgaatac acctatgtca aatttcattt
    16861 ctttgtataa ttgttttaat tcacgttttc tatccatgat tttagatacc tctctttata
    16921 attgatatgt aatatgtaag tgtaaaaata tattacatat aaattataaa gagattaatt
    16981 ttatatgtaa atagttttta taaatttaat tatgaattag tcatcaataa acaagaaatt
    17041 tagctgtaga aatccattat aaaggctatt ataaatttta atatgctaaa aatataaaaa
    17101 gaatagttga cagtgtagaa aatatggaat aaactaaaag tgtaatagta atatgaaatt
    17161 atatattaca ttgaaaggtg tgaatgatta ttatgaataa aaaaatagaa ttaataggga
    17221 actttgcaag tgattggatt aggtatagtg attatgaata taaaattact gaagaagatg
    17281 aattatatat agttccaaca gaagatgctg tgtttaccat gtataaccca tttgatgtag
    17341 cagatgatat aatagtagat ataattcgca taggacaaga ggcattacaa gatatatcaa
    17401 aagagacaaa agaaatgaaa ctaaagaaag aagtacttga atttgtaaaa aagtatggat
    17461 tattgggttt aatatattca agtgtatata atagagatat agttggagaa gaaagagtac
    17521 tgatgattga aaagaattat attacaaaag agaatatatt gagtacagat gaatatgtaa
    17581 acatgtttat accatttgca agtgatgaag atgtgtattt taaaaagtat agaaggggta
    17641 tagatgtatc aaagagagaa gaatctccaa agttttttgg aaagagacca ttgattttag
    17701 atttagtatt ttcaaagttc tacactgaac aactagattg gattgtaagt tttgctaaag
    17761 atatgtgtaa gcattttaat caaatttgtg tatataaaaa tacatctaat ctattaactg
    17821 aaagtgttac tattatgtct ggagggttta atcctcaaaa gataggattt tctataagcc
    17881 aattagataa accaattatt gcatgggagt ttgattcttt gaaaacagct attgagacta
    17941 tctatgcctt tgcaattaca agtgaacctt cttctttaaa taaatgcaag tactgtggta
    18001 aaatttatat tcctacaaat tctaaatctc aatactgttc accttcttgt agaaattgct
    18061 caaatgtaaa gaaaagtaga agtagagcca aaaaagataa ataaaattaa taaatatatc
    18121 attgtttaga ttttagtaaa taactactga attaaaagtt gttatagaaa attatatatc
    18181 taaatgatgg tatttttttt gttgagaaaa ttgaaaagtt taattgagat agtaattttg
    18241 agttgacaaa aaccactttt aacactacta ttataagtgg ttggcaaaat aatatatact
    18301 aatttaagta tagcgtaaaa aacaaaaaaa gtcaatggaa aaaataaaaa aggagaattt
    18361 aaaaatgata tcacctatta ttttgagttc tataaatcag aatttaaagg aaatagaaag
    18421 aaatgaactg cttgaaacta atatagaatc tggagattat ggtctagctc tttctgaatc
    18481 tgatgtaaaa gatataataa attcgagaga taatacttta aaggggtatg gaagaataga
    18541 attagatata aaagttacaa aacagttaat agaaaatata tatacatcac aatatacaaa
    18601 tgtggacaat tatttagagg ctattaatga tatgcaagaa attttttatt atttaaaaaa
    18661 tgaaactgat gacaagatat gtgatgatga gattatagaa atactgggtg aattttatga
    18721 aaaattttca ggcaatatgg acaatgtaag aggagaagca gacgaatttg caaagaaatt
    18781 taaatttgga gaagtttaat atatgataag agagattttg tatgttaaat aatttaagta
    18841 atattaatat aaacgaaaat aatttaataa agaaccaata ttccatatct ctacttaagg
    18901 aatgtctaaa ttgtaaagtg atagatgaaa gagaagtata taatatacaa caagaaatat
    18961 ctctaatact tatggatttg attaaaaaat atacaaatgg tcaaagcaca tcagtaaaaa
    19021 cagaagttgc cgaaaaactg ttgatttcaa tatggtatgc tattgatgca tatataaata
    19081 agcttggaga tatagaaaaa agagttgtgg agttgactgc taagaatgtt aaaaaggtgt
    19141 accttggagg cattgaaata ttagaggaag agttagttag gctaaaagat ttttataaac
    19201 taatgatgag tagaaaattg gatactaatt caatagcata taatgatacc ttgatggagt
    19261 tatcaagttt ctttaaaagt tatattgtaa aatttgaagc tcaggacgtt ccagtaagta
    19321 tagattatcc attggcacta gatgatatgg atgttaaggg tatttattat gtgaagaatt
    19381 atattgagag tatagatata gaaaatagat tttgtaacct atttaagaaa aaagatagag
    19441 aaaaattatt ttatgactat ggggtgactt ataaaataga ttgtgatgtt gtacttgtaa
    19501 atttatttga acttattata aataattgta ttttttctac tattttagga aatgaagtga
    19561 ctaatttaga aatttcaaaa tatgagtatg aatttttaga aaaacaattt aaaaatatgt
    19621 acaataatat tactgaagat gaagttatag gctctgaaga aagtaatata attgaattga
    19681 ttgaagtaga tgatagtttt aacactgaag tagatagtaa tttggattat aatgattttg
    19741 aagaaattaa cagagaaatt agaataaata aaaaaataac tttactactt atggaagcaa
    19801 ttgaagtatt aataaaaaga ttaagcatag aagatgaaga tgtaataaga tatattaaaa
    19861 aatatgaaaa gatatttatt gaatctgtgc taagctcaat aaaaagtaac acttttaaaa
    19921 gtatggtaac tgtgagtaaa aataaaaagg aacacttaga agattatatt gttgatgatg
    19981 aaagtaaatt ggatgatgaa tcgtttagag agatattcaa tgagatatta gatagtacat
    20041 ctataattga aaaaataaga ataataaagg aaaatatcaa tgctaaaaaa gactttatcg
    20101 atattcttga atctgaatgt ttatttggag aagagtattt aatgttgttt gcatcattaa
    20161 gtgaattaga attggcaata ttgggtagtg tagtatttta tgaagatatt agaatgagag
    20221 aaattaatat attagaattt atgttaaatg agaaaactga aactacttta tggaagatag
    20281 agtacattaa atttatgaag aggcaaagtg aagataagat atactccata gaagagcata
    20341 tgaataaaat taattaacaa aaaattgtga ataaatagaa tgttagtata taatataatc
    20401 tgtaaagcat atcaaaaata catatatttg gaggaaaatt atgaaagagt atatcataaa
    20461 taatgttgaa gaaataagag aagaattaat tgatttatct aagaaaatat gggaaaatcc
    20521 agaattagcc tttgaagaaa agtatgcaag tagcattcaa aaagaatacc taaagtcaaa
    20581 aggatttaaa attgaagaag tagaaaattt acctactgga tttatagcaa gttttggaga
    20641 gggtaaacct gtgattggca tacttggaga atatgatgct ttaccagagc tatcacaatg
    20701 tgtaagtgca gaaagaaagc cattggtaga aggaaaagct ggacatggat gtgggcataa
    20761 cctgttgggt gtagcaggtg taggagcagt tgtttctata aaaaaattaa tagaagaaaa
    20821 taaatttaat ggaacaataa aatattttgg ttgtcctgct gaagaagaag gtggaggaaa
    20881 gacagttatg tgtataaatg gctgttttga tgatgtagat tgtgcattta catggcatcc
    20941 ttttgatata aatgcacctt ggagaggtgg aagtctggca aacttatctg taaaatttaa
    21001 atttaaaggg ataacagccc atgcagctca agctcctcat aatggtagaa gcgcacttga
    21061 tgctgttgaa attatgaatg ttggagctaa ttatttgaga gaacatgtta ttgactctat
    21121 aagaatgcac tatgttataa ctaatggtgg tggaagacct aatgtagttc ctggatttgc
    21181 tgaaagttgg tactttatta gaggtaaaaa agcaaaggat gcagaacacg ttcttgatag
    21241 attaataaaa gtagctcaag gagcagcaat gatgacagaa actgaaatgg agtataaggt
    21301 tacagatgga atatatgact atataccaaa ccaatgtttg actgatttag tttatgacaa
    21361 tatggttttt gttggttgcc ctaaaaatac tcctgaagaa gaagaatttg caaaaaagtt
    21421 gtgtgatact cttactagag aagaaagatt aggtgtagca acatcattac aaaatgataa
    21481 atctattgta gaaagctata tacacaagga aatagttgac ggtgacaaag ataaaggact
    21541 agctggttct actgatgttg gagatgtaag ttatgtaata ccagtggctc aatttgcaat
    21601 ggcagcttgg cctgttggaa ttgcaagtca tacatggcag tcatgttcat cagcaggttc
    21661 aaatatagga ttttctgcta tgataaattc agctaaagta ctggcttgta gtgcttatga
    21721 tgtatttatg gatactaaaa ttattgatga agcaaaaatt gaatttgata aatcattaga
    21781 tggacaaaaa tttaaaccat tggtataatt ttggtaataa aatatttctg gggggaatag
    21841 ctatgagaga aaaagttgaa aaagtgcttg aggaaaagat aaaaccagta ttacaaagag
    21901 atggtggaga tgtagagctt atagatgtaa atgaaaatgg tgtggtttta gttagactac
    21961 aaggggcatg tagtgggtgt ccaggagcga ctatgacaat caaagctatt attgaaaatg
    22021 ttttggtgag tgaagttcca ggagtaactc aagtattagg tgtttaatag ttagcgttta
    22081 ttaaatgtat gttatacata agtatttaat aaagaagtaa aaaaaatagt gaagcaatat
    22141 atatgaaaaa gtatgagaaa gataataaaa cattatagaa ataatgaaat atttcatatg
    22201 tatat
//
LOCUS       DAENCV010000056        22108 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAENCV010000056 DAENCV010000000
VERSION     DAENCV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.057"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            834..2504
                     /locus_tag="KQD82_002198"
     CDS             834..2504
                     /locus_tag="KQD82_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG4380668.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHVDIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2626..3360)
                     /gene="pflA"
                     /locus_tag="KQD82_002199"
     CDS             complement(2626..3360)
                     /gene="pflA"
                     /locus_tag="KQD82_002199"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG4380669.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3520..5751)
                     /gene="pflB"
                     /locus_tag="KQD82_002200"
     CDS             complement(3520..5751)
                     /gene="pflB"
                     /locus_tag="KQD82_002200"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG4380670.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6044..7105
                     /locus_tag="KQD82_002201"
     CDS             6044..7105
                     /locus_tag="KQD82_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG4380671.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTSKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7196..8809
                     /locus_tag="KQD82_002202"
     CDS             7196..8809
                     /locus_tag="KQD82_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG4380672.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9084..11069
                     /locus_tag="KQD82_002203"
     CDS             9084..11069
                     /locus_tag="KQD82_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG4380673.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKDLLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11074..11508
                     /locus_tag="KQD82_002204"
     CDS             11074..11508
                     /locus_tag="KQD82_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG4380674.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11537..12097
                     /gene="srlA"
                     /locus_tag="KQD82_002205"
     CDS             11537..12097
                     /gene="srlA"
                     /locus_tag="KQD82_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG4380675.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12161..12529
                     /locus_tag="KQD82_002206"
     CDS             12161..12529
                     /locus_tag="KQD82_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG4380676.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12554..13120
                     /locus_tag="KQD82_002207"
     CDS             12554..13120
                     /locus_tag="KQD82_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG4380677.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13161..13526
                     /locus_tag="KQD82_002208"
     CDS             13161..13526
                     /locus_tag="KQD82_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG4380678.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13627..14400
                     /gene="srlD"
                     /locus_tag="KQD82_002209"
     CDS             13627..14400
                     /gene="srlD"
                     /locus_tag="KQD82_002209"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG4380679.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14749..16893
                     /locus_tag="KQD82_002210"
     CDS             14749..16893
                     /locus_tag="KQD82_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4380680.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16981..17316
                     /gene="spoIIAA"
                     /locus_tag="KQD82_002211"
     CDS             16981..17316
                     /gene="spoIIAA"
                     /locus_tag="KQD82_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG4380681.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17330..17758
                     /locus_tag="KQD82_002212"
     CDS             17330..17758
                     /locus_tag="KQD82_002212"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG4380682.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17781..18545
                     /gene="sigF"
                     /locus_tag="KQD82_002213"
     CDS             17781..18545
                     /gene="sigF"
                     /locus_tag="KQD82_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG4380683.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18825..19262
                     /gene="spoVAC"
                     /locus_tag="KQD82_002214"
     CDS             18825..19262
                     /gene="spoVAC"
                     /locus_tag="KQD82_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG4380684.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19429..20442
                     /gene="spoVAD"
                     /locus_tag="KQD82_002215"
     CDS             19429..20442
                     /gene="spoVAD"
                     /locus_tag="KQD82_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG4380685.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20456..20818
                     /gene="spoVAE"
                     /locus_tag="KQD82_002216"
     CDS             20456..20818
                     /gene="spoVAE"
                     /locus_tag="KQD82_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG4380686.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21001..22017
                     /locus_tag="KQD82_002217"
     CDS             21001..22017
                     /locus_tag="KQD82_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG4380687.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8620 a         2479 c         3848 g         7161 t
ORIGIN      
        1 atggatgtaa taattaaaat aaaaaatatt atttttattt ataaacattg acattctaat
       61 tttgtagtgt aatcgtattt tttattaatt cttacaaaat caatgaaaat aagtatatcc
      121 attttaaagt acataaattt aaaaatccag aaaaatatac ttaaattatg taagtaaaag
      181 aggggttgaa tcagttgaaa atgtaatatt agtaaatagt acatcaatcc tatataccct
      241 gtaagcaact ctattttcat gtactaaaaa tgtacctatt ttattaactg aaaagaataa
      301 attaaatgta agtacataag aagatagaaa aaattaaaaa caaaaaatat ttatataaat
      361 aaatataaaa aatgatataa tccctattaa aaaatttata gatgatgaag aaatatcaaa
      421 atcttatatt ataggaagag acacttcttt gccaaaatct atagaaaaac aataccaaat
      481 ccaaagagaa taggcggaga aaaaaataaa ataaattcaa gagtaataga aaagttttat
      541 agaataaaga actgataact ctttatataa cgaaaaatag aattttaaga gaaaatgatt
      601 taataaatga tttaacatgg tgttttagca ggtaaaaata aatcaccagt aatgattata
      661 gaaaaattac tttatgaatc tcaaaaaact tagttaagag taagagattt gataagataa
      721 ctaaagttgg ggaaatgtgt ttgatgagac aaaaaattag taaaatagta taatctagat
      781 aaggatattt tgttgattaa actacactaa tagtaatatt gtagggggaa aagatgagag
      841 aagaatcgaa ctctttaagt ttagaccata tagaaatttt ctttgaactt ttatctcaaa
      901 gtacagaaga ttatatattt ttttgggata taaataggaa taaatttaaa atttcttcag
      961 caatttttga tgaatttaac ttaagtaaag agatagaatc tgatttagtc aattgttgga
     1021 gtaaaattgt ttatccggat gatgttcaaa tatggaaaga tgatatacaa gaattactac
     1081 atggaaaaaa aggtgagcac aatttagaat ataggttgat taataaatat aaagaaatag
     1141 tatggatttc ttgtagaggt aaagtttatg tgtcggatga cccaaaaact atatttctag
     1201 ttgggagaat aaagaatatt ggtgaaaaaa ataaatttga ttctatcaca ggaacgtgga
     1261 atagagaaca atttgaacat agaatgaatt atcttataaa agagaaaatt tataaaaatg
     1321 gtgctatgtt tattatggat attgataatt ttaaaaatat aaatgaaaaa tatggacatt
     1381 cttatggtga caaagtattg cgtgcaattg ctactgaagt attagaatat cttccaaaag
     1441 atgtaagatt gtataggtta gatggagatg aatttgcatt tttttaccct atgtgtacta
     1501 aagaaactat agagaaaata tatgaaaaga tacagatgta tacaaatact cagcatgaaa
     1561 ttgaatcaaa taagtactat tgcacagtga ctgcaggcgt tgctatgtat ccagaagatg
     1621 gagataatta tttagattta tttaaacatg ttgatattgc attggatata gcaaaaatta
     1681 gtggtaaaaa tagaattaag tttttttcac aagagctcta tgaaaataaa ctaaaggtaa
     1741 tttcaatgca acaaaaacta agagagtgcg ttgaaaataa ttttaatgac tttgagctgt
     1801 tttttcaacc ccaggtaaat gctgttacaa aagaggtaat aggggcagag gtacttttaa
     1861 gatggcattc gagtacttat ggagaagtat ctcctgttga atttatacca atactagaac
     1921 agagtaattt aattatacca gttgggaagt ggattataaa agaagctgta aaacagtgta
     1981 aagaatggca taaaataaat ccagacttta aaatatctgt aaatgtatcg tatattcagt
     2041 taaaggaaga tttttttaga gattttatag tagaatgttt agtagagtat caattaagac
     2101 ctgaattttt aattttggaa ttaacagaga attgttggat accagatata aatttactaa
     2161 atgataaatt tattagttta aagggtatag gagtatatat tgcgatagat gactttggta
     2221 caggatattc atctttaaat taccttaaag aactgtctgt taatataata aaaattgaaa
     2281 gaagttttgt taagaatatt acatataata gttatgaata tacttttctt gaatatatca
     2341 ttaaattagc tcatattata aatttaaaag tttgtgttga gggaatagag tcttatgaag
     2401 agtatgatat agtaaaaagc ttaggtgtag atattattca gggtttttta tttggaagac
     2461 ctgtaagtgc ctctgaattt tataaatttc agctatcaaa gtgatgtata taaaatgaga
     2521 tatatagtat ataaaaatag ggcaaagtgg aagttttgaa gtttattaaa aatttacact
     2581 ttaccctatt ttgttatgtc ttctataaag gattaatata ctacactaac tattatatac
     2641 tttcactcca aatttagaga ttatttcact agctttttgg acatcttcct tagtggcatc
     2701 aggaacatct tttagttcat aatcgatacc taaactttcc catttgtgta ttcccataga
     2761 atggtatggt agcaattcaa atctttctac atttttcaat gtagctacaa attgacctag
     2821 tttttctaaa ttatctatat catcagtaat gccaggaact aaaacatgtc ttatccatac
     2881 aggaatattt cttgattcta agtgtctagc aagttttaat gccttatcca taccaacacc
     2941 agtcaaatca atagattttt cctcaaccat atgttttaaa tcaagtaaaa ccaaatcagt
     3001 attatctaaa actgggtcta tagtttctat atctacaaat ccagatgtat ccaagcaagt
     3061 gtgtatatca ttttcttttg cttttttaaa taattcactt aaaaattctg gttgaagagt
     3121 ggattcacca ccagaggcag ttatacctcc accagaaaac ttcataaaag aagtatactt
     3181 aagagcatcc gttattattt catcaactgt gtattctttt cctgatttaa tatcccatgt
     3241 atccctgtta tgacagtatt tgcatctaag aggacacccc tgaaaaaata atatatatct
     3301 tattcctggt ccatctacag taccaaacgt ttctatagaa tgtatctttc ccttaatcat
     3361 agagttcacc tcacaatata ttttaataac cttatcaata gttataatat ttctctaaaa
     3421 aaggccatct tataccagaa ataattcaaa tataagatag ccttttattt ttatttttta
     3481 cacagtagta atcttagtta aagtataaaa atttaaagac taagccattt taccgtggaa
     3541 tgttctgttt ataacatcta attgttgctc tttagttaat tttatgaagt taactgcata
     3601 tccagatact cttatagtta attgtggata ttcttctgga tgttccatag catcttctaa
     3661 agtagctcta tcaaatacat ttacatttaa gtgatgagca ttttgagcga agtatccatc
     3721 catcattgca gaaagattgt ttattctttc agtcatatct tttcctaaag cagcaggtac
     3781 tatagagaaa gtattagata taccatcttg agcatgttca tatggtaatt tagcaactga
     3841 agataatgaa gctaaagcac cattagtatc tctaccatgc attgggttag caccaggagc
     3901 aaatggagca ccagctcttc taccatctgg tgtattacca gttttcttac cataaacaac
     3961 atttgaagtt attgtaagta tagattgagt tggataagaa tttctataag ttttattctt
     4021 tcttatttta ttcataaagc tttcaactaa ctctacagct atactatcaa ctctatcatc
     4081 attatttccg tattttggat aatctccttc tatttcaaaa tcaacagcta ttccttgttc
     4141 atttctaata gttttaacct tagcatgttt tatagcagat aatgagtctg cacaaactga
     4201 taatccagcc ataccacaag ccatagttct aaatatatct ctatcatgta atgccatttc
     4261 taatgcttca taagaatatt tatcatgcat ataatgtatt acatttaaag tatttacata
     4321 taatgttgct aaccaatcag tgaatggttc aaatttactc ataacttcat cataatctaa
     4381 gtactcagaa gttataggtt caaatcttgg tccaacttga acaccagatt tctcatctac
     4441 accaccattt atagcgtata ataaagtttt agctaagtta actctagctc caaagaactg
     4501 catttgttta ccaattctca ttgcagatac acaacaagct ataccatagt catctcccca
     4561 gtatgctctc attaaatcat cattttcata ctgtactgaa cttgtatcta tagatacttt
     4621 agaacagaaa tctttgaatc cttgaggtaa tttagttgac catagaactg ttaagtttgg
     4681 ctctggagaa ggtcctaatg tatatagagt atttaacatt ctaaatgagt ttttagtaac
     4741 taatgttcta ccatctattc ccatacctcc tatacactca gttacccaag ttgggtctcc
     4801 agagaatagg ttattgtaat caggagttct taggaactta accattctta atttcataac
     4861 aaaatggtcc atatactctt gtaattcttc ttctgttacg attccagctt ttaaatctct
     4921 ttcaaagtat atatctaaga aagtagatgt tctacctaaa gacattgcag caccattttg
     4981 gtctttaaca gcacctaaat aaccaaagta taaccattgt acagcttctt ttgagttagt
     5041 tgctggtttt gagatatcaa atccataaga ttcagccatt tttttaagtt catttaaagc
     5101 tgatatttgc tcagttattt cttctcttaa tctgataact tcctcatcca tgcaagaaac
     5161 ttctaatgat ttcttttgct catttttatc ttctattaaa gcatcaacac cgtatagagc
     5221 aactcttctg tagtcaccaa ttattctacc tctaccataa gcatctggaa gacctgttat
     5281 tataccagat tttctagcag ctctcatttc tggagtataa acatcaaaaa ctccttggtt
     5341 gtgtgttttt ctgtatttag taaatatatc ttttatttct gggtctaatt catatccaaa
     5401 agcttcgcat gaattttcaa ccatctttat accaccatat ggcatgacag ctctttttaa
     5461 aggggcatcc gtttgtaacc caactattgt ttctaattct ttatctaaat atcctggagc
     5521 atatgcagct attccagaaa cagtttttgt atcaacatct aaagtaccac cattttctct
     5581 ttctttttta aataggacca ttgcttcgtc ccataatttt ttagtatttt cagtggcacc
     5641 agctaagaaa gagtcatttc cctcatatgg agaatagttt aattgtataa actctctaac
     5701 atttatttct ttagtccatc ttcctgtttt aaatccttgc catgcattca ttaagtattt
     5761 cctccttaga tttaaatgat aaataatact attaaaatta atttttatat acttaattta
     5821 ttttatatat ttttttaaaa atatattttc taatataata atagcatatt attcgtatcc
     5881 tgtatacata aaaaataaaa aattgtcgct ttaaagtata aaacagatta tatgttaata
     5941 atgtggtgaa attataccca tatataaata aaataaaaca aataaatagc taattaagga
     6001 gaaaatattt tttatttttt gtttaggata aataaatttt attatggaaa aaagaaaaag
     6061 catacatctt cacaaactga atatttattt aataggggga ggtgatagat tgtttatcac
     6121 agtggtatta tttttagtag gtttcctatt aattactaaa ggcgcagata tctttataaa
     6181 ttgtactgta gagataggta aaaaaacgaa tatatcagaa attatacttg gagctactat
     6241 agttagtttt gcaacaacat tacctgagtt tacagtgtct ctacttgctt ctattgatgg
     6301 tcatactact atgagtttag gaaatgcagt tggttctata atatgtaata cagggttagc
     6361 attagggttg gttgttttta taagtccatt taatgtagat aaaaaaatgt ttttttctaa
     6421 gtctttacta ttaatagtat ccgttatagt tttaatttta ttgagtttag atggagttat
     6481 aacaagaggt gattcattat tacttataat aatattaatc ttttatatgg ttaataatta
     6541 tagaagtgtc gttggaaaat ctgatactaa aaatagaaat attaaaaata acactagtaa
     6601 agacatatct actaaaaaag ggaaaaatta taggggtttt tccgtattag aaattgtaaa
     6661 aatactatta ctttttgcta caggattgat aatgatgata ataggttcac aaatccttat
     6721 agagagtgga gtaataatag ctagtttttt aaatatacct cagggtatag taagtttaac
     6781 tataatagca cttggaactt ctctgccaga gatagtttcg tcgattactg ctataagaaa
     6841 aaatcatcat gaaatatcag taggaaatat tttgggtgca aacatattga atatagtatc
     6901 agtaatagct gtatcagcaa taccaaataa cataccaata ctatctcaaa atagacagtt
     6961 ggatatacca ttcatgatat tattattatt aatagtaata ataccaacac ttaaatctaa
     7021 taaacttagt agaatacaag gtatattgat gttattcaca tattttctgt acatatcaat
     7081 tttgtatttc atgtatatta tttaactata ctgtttaaaa tattaattgc agaatatcct
     7141 aaaaaagtat ataatggata ggttaagatt aattgataac ggagaggaat aatatatgaa
     7201 tataacaaag tttgaagatt taccaataag tgagggaata aaaaaagcta tagctgaaat
     7261 ggggtttgaa gaaccatctc ctatacaagc acagtctata cctgcgattt tgtcaggtaa
     7321 agacgttata ggacaagctc aaactggtac gggaaaaaca gctgctttta gtatccctat
     7381 actagaaaca atagacccaa ataatagaag tttacaagct gtagtgcttt gtccaacaag
     7441 agaactggca atacaagtat caacagaaat aagaaagtta gctaagtatt cgcacggaat
     7501 aaagacatta cctatatatg gtggtcagcc aatagataga caaataaaat ctctaaagag
     7561 tggtgtgcaa gttgtaatag gtacaccagg acgtacaata gaccatatca atcgtaaaac
     7621 attaaagatg gataatgtaa aaatgattat attagatgaa gcagatgaaa tgctagatat
     7681 gggatttaga gaagatatag aaatgatttt aagtaagatt ccagaagaaa gacaaacaac
     7741 tttcttttca gctactatgc caagaggaat tttggaatta actaagagat atcaaaaaga
     7801 cccagagcat ataaaagttg ttagaaaaga acttacagtt tcaaatacaa agcaatacta
     7861 tatagaaact aggtcatcaa ataaattaga agtattatgt agattagtag atgtatatga
     7921 ccctaaacta tctgttgttt tctgtaatac taagagaaaa gcagacgaat tggtaggaga
     7981 tttacaagca agaggatatt ttgcagatgc attacatggt gacttaaaac agactcaaag
     8041 agatatagta atggataagt ttagaaatgg tacaatagat atacttgttg ctactgatgt
     8101 tgcagctaga ggtatagatg ttgatgatgt tgaatgtgta ttcaactatg acttaccaca
     8161 agatgaagag tattacgttc atagaattgg tagaactggt cgtgctggta gagaaggtat
     8221 gtcatttact tttgtatttg gaaaagaaat gagaaagatg aaagatatag aaagatatac
     8281 taaatctaaa ctaataaagc ataatatacc aacaataact gatgttgaag agaaaaaagt
     8341 aggtactttc tttgcacaag ttaaacaaac tatagaagaa ggtcacttaa ctaaacaatt
     8401 acaatggtta gaaggtttct gtaatgatga agattatgca atggttgata tagctgctgc
     8461 acttgtaaaa ctatctctag gtgaagaaat gaaagaagaa ataatagaag aaaaacctag
     8521 aagagagcgt ggagatagaa aaggtggtac tggagctaaa gatggtatga ttagattatt
     8581 tatcaatatt ggtagaaatc aaagagtaca agcaaaagat atagttggtg ccatagctgg
     8641 agaagttgga ataccaggta aagtagtagg tactatagat atatatgata agtatacttt
     8701 tgttgaaata cctaaaaaag atgcaaaaac agtaattgaa aagatgaaag atattaagat
     8761 aaaaggtaat aagattaata tagaaaaagc aaataagaag aaaaaataac gtatagtata
     8821 caaaactata attagaaaat atataaaata atatattgga tatttaaaaa gcctgatgtg
     8881 ttttcaaaac agatagtaga taatctgtaa gagttaacag cataaggctt ttcttgtttt
     8941 atatgatgtt ataattttcc agattattgt gaaaaatgta tattagttcc tttttttcaa
     9001 atctgatttg aaaaagagaa tattaattat ctatatagtt tagattataa taaaaaatgt
     9061 aagataacaa aggggagaaa aagatgtcaa tgacttatat ggaccctagg gtttttttga
     9121 ttttagaaat ggtgttagaa gaagacacaa atactctaga acaattttca aatgttttgg
     9181 gtgttagtac taggactatc agaaactata taaaccagat taatcgagaa ttaaaaggtg
     9241 gtattgctaa aatagttaaa aatgaaaaag gtacatatag tataagaatt gaggatgaaa
     9301 gtaaattaat tgagattgta aattttaata gaggaaagct atcaaatttt ataaatttaa
     9361 attcacctga tgaaagaata aattatgttc ttgatatttt aactatgacc aacaatccaa
     9421 ttaccataga tgatttggct gaagagatat gtataggaag aacaacatta ataaaggatt
     9481 taaaaaaagc agacagaatt ttagctgaat atgatttaga attaaaaagg aagccaaata
     9541 taggtatgat tttagttggt gaagaaatgg atataagatt attaatcctt gatagattgt
     9601 atgaaaatta tattgatgtc ttggaaaacg ttaatcagat aaatttagtt aaagattatg
     9661 atattgaatg tcttagagat gaattaaaaa agttatttaa aaaagaagaa ctctacataa
     9721 cagaacaggt cttgagagat gttgaaagat atataatcat gtctgtctta agaaatctaa
     9781 atgggtacaa atttgaaaag atagataaga gatttgaagt gataagatgt tcagatgaat
     9841 atactttagg attaaagctc aaagatttat tggaaaagat atttaatata gttttaaatg
     9901 aaaaagaaac catatttttg acaatgccat taataggtag aaaagctcct agtacaaaat
     9961 tagcacttag cagtataaag ataaatgata gcgtaaagga agttgtagat gaagtaacta
    10021 gttttttatt agagacctca gggattgatt ttaaagaaga taaaaagctt atagaaaatt
    10081 tggaatatca cttgtatttt gcacttaata gaatgagatt caacataagg gtaaaaaatc
    10141 ctcttttaca agaaataaag gaaagatata catttccata tagtgttgca aagatagctg
    10201 caatgataat tgaggaaaag tttgacttga aaatatgtga ccacgaaata ggatatatag
    10261 cattacattt tggaagttat tttgaaaaaa attcaagaaa agttctaaat gtaaataggg
    10321 tagctgttgt ttgtggaaca ggtcttggga ctgcacaact tattagaata aagataggaa
    10381 aaatacttgg agaaagtgta gaagtaaata ctttttctga tatagaagct aaagctaatt
    10441 tagatttatt aaagtattat gatttagtat ttacaacagt agatttcaaa acagacttag
    10501 atgttccaat ttttagagta aatgcgttat ttaatgaaga aagtataaag aaagagatag
    10561 aaaatgcaat acttttgaaa aatagtataa atcttaagtc tataaaaaag attgaaaaaa
    10621 atatcccttt tataggtaaa ttaattgaag aggataagtt ctttatattg aataagagta
    10681 cgtttatgga aaatttagaa gatatgttag aaaaattagt tcataaagga attattgata
    10741 tagagttcaa aaaaaaggta ttaaaaagag aaaagaaatc ttcaacagca ttagataatt
    10801 atatagcatt gcctcactca gttaatacta atggggaaaa tctttatctg gcatttggaa
    10861 tattagaaaa accagtgatt tgggatacaa aggaaattag aataataata ttgatgataa
    10921 ttccagataa gaatgtagat agtacagacc tgataataaa aagttatgaa gaagtacttg
    10981 aagtagggag agataaaaag atggtagaag aacttgctaa ggcaaaatca tttgatgaat
    11041 tttataaaat aattaccaag aggaggtaat aatatggacc cagtaatttt tattatagca
    11101 tcacttatta cagtgtattt attaaatatt tttcttggat acttacagct taaagatttt
    11161 aataaaaatt atattgaact aaaaagaaaa ggaagagtgg caattggcag aaaaaaagga
    11221 agaatttcat cgggtacaat tgtgttgata cttataaatg atgatggtgt gattgtagag
    11281 actagaaaaa tgcaaggtgt tacagtattg gcaagagtaa agtcatttga aggtttggta
    11341 ggaaagagct tagagtatat agaaaaatca gatttaactg tttacaataa actacttaaa
    11401 aaagcaatac tagatgcagt caaacagtac ataacatttg agaaaaataa atgtgaagat
    11461 acaaataatc tgagtactga tgaagagtgt aggatggaag tagtttaaaa aaatatattt
    11521 ataaggagga gttaatatga cgcaattttt tcaagtatta gcaagtggtg cagatagttt
    11581 tatgaattta tttagggcag gtgggcaaca atttgtaggt tttgtaacag atatagtacc
    11641 acttttagta gcattattag ttacaatgaa tgcaattatc aactttattg gtacagatag
    11701 agtcgagaga cttgcaaaaa aatgttctgc aaatatattt actagatatt tgatattacc
    11761 agtccttggt acatttgtat ttgcaaaccc aatgacacta tcattaggaa gattcttgcc
    11821 agaaaagttt aaacctagtt actttgcagc agcaagcttt tcatgccata caatgaatgg
    11881 attattccca catatcaatc caggtgaact atttatatat ctaggaattg caaatggtat
    11941 aacaactttg ggattctcaa cagcagattt agcagttaga tatttgttag tgggtatagt
    12001 tgcaaacttt attaaaggtg tagttactga ctatacaaca aaatttgtag agaaacaaca
    12061 aggaatagca ttaaattcag atataaagat tggataatta ctttaaggta taaataaatt
    12121 tttcaaataa aaagatagtg tatagaggag gataaattat atggcattta acaaagttaa
    12181 aatagtaaaa ggttctggag gatggggtgg accattgata atagaaccaa cagaaaagaa
    12241 gaataaggta gtatatgtga ctgggggagc aaagccagaa actgcagtaa aaatagctga
    12301 acttacagga tgtgagttaa tagatggatt ttcacagggt gttaaagatg atgaaatcgc
    12361 atgtgtaata ataaattgtg gtggaacatt gagatgtggt atatatcctc aaaagaaaat
    12421 accgactata aatataatga aaacaggaaa aagtggacca ttagcaatgt ttataaaaga
    12481 agatatatat gtttcagggg taaaaccaac aaatgtagag cagttataat tgaggtagta
    12541 ggagggaaga aatatggctt atgatactaa aaagaagata agtgagcaga gtcaaggaat
    12601 tgttgcaaaa ataggtatag cactaggaaa tgtgacaagt actctttttc aagctggtag
    12661 ggatactata gatacattaa tacatactat actgcctttt atggctttcg ttgcagtcct
    12721 aataggtata ataaatcaat cgggtatagg aaattgggta gccaatttac ttactccatt
    12781 aatcggaaat gttggaggtt tagttgtctt atcaataata tgttcatttc cgttgttgtc
    12841 tccgtttctt ggaccaggag ctgttatagc tcagatagtg ggggtattgg ttggtacaga
    12901 aataggaaaa ggaaatattc caccaagttt agcattacca gcattgtttg caataaactc
    12961 acaagcagct tgtgacttta taccagtagg tcttggtctt gcagaagctg aacctgatac
    13021 agttcaagta ggagttccgt cagttttata ttctagattt ttaacaggtg caccaactgt
    13081 tctattagca tggttagcaa gctttgggtt atataaatag aagaatactt tataaataat
    13141 tagagagagg ttcagttgat atggaattaa tatacagtac tcaaataaat aaaataggag
    13201 ataatgcaaa tgaattttta caacataata tgtttgtaac tttcaaagat aatgctcctc
    13261 aagagttaga aaattactgt tatatacatt ctgaaaataa tttagtaaaa gaaatactta
    13321 cggatgatgt gctatcaata aataatgttg attacaaaat tacttctgtt ggtgagttag
    13381 taaaccaaaa tctttcagaa ttaggtcata taacatttaa gtttacaggt gaaaaagatg
    13441 gagcaattgg aggaacctta tatcttgaag aaaaagaaat agcacctatt ggcgtaggta
    13501 ctatactaaa aataattaga aaataaaatt taaaacgaat ctaatcatat tataagtaaa
    13561 ttatagataa aagtcaattt aactaatcaa gttaactttt aatgtgataa aaaaggggaa
    13621 atgaaaatga gaaaagtggc tttagtcgtt ggaggaggaa gaagtttagg tgaatattta
    13681 tctaaacatt tagctaaagt aggttatgat gttgctattg cagatttaaa tgaagacaac
    13741 gcaaaaaaag tagctgaaga aataaaaact gaatttggtt gtaaatctgt aggatatagt
    13801 tgtaatgcca tagatgaaag acaggttata agtactgtca aagctattga aaatgatttt
    13861 gatagaatag atttattagt gtacaatgca ggagtggctg taagtaaaaa aataactgat
    13921 tttggataca aagattttaa atttattgta gacgtaaatt taaatgggta ctttctattt
    13981 gcaagagaag tatctaaagt catgatagca aaaggtattg agggtagaat aatacagata
    14041 aattcaaaat caggtagagt gggaagcaag cataattcag gatattcagc aggtaaattt
    14101 gctggagtag gacttactca aagtttagcg ctagatttgg cagaacataa gataactgta
    14161 aatgcactga tgctcggtaa tttacttgat tctgatatgt ttgaaagttt aattcctcaa
    14221 tatgcagtaa aattgggaat acctgaatca gaagtcaagc aagtttatat agataaagta
    14281 cctttaaaaa gagggtgtag atttatagat gttgcaaata tgcttacatt ttatgcttca
    14341 aaggaagctg aatactgcac tggtcaatct ataaatatta ctggtggaca agttatgtaa
    14401 ggtttttatg gagtgtctca agatgaactc tttagttcat gaggcacttt tttgtataaa
    14461 attaaatata ttttcagcat ttaaacaatg aaaaagaggt ttatctttat tttgagatag
    14521 cctcattttt gtaattaaaa aacatagtgt ttaagtaaac atataagatt gttagttttt
    14581 taaaagagaa ttttttaata atattatatt tatgaagtat ttttaaactt aatttgataa
    14641 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
    14701 tatatgttaa tttttaataa ggtaagaaag tagaaagaag gatattttat ggatataata
    14761 aaaaaattaa gtcatgggtg tacattagat tgtcatgact gttgtaaatt caatgtttat
    14821 actaaaggaa ataatgtagt aaaaattgaa ggagataaaa atcatcctta tacaaaaggt
    14881 tttatatgca aaaaagggat ggcccattta gatagattaa atcataaaga tagaataaag
    14941 actcctatgc ttaaagttga tggagtatgg aaagagattt cttttgataa agctatagaa
    15001 ataatggcag aaaagcttac atactataaa gaaaagtata catcaaagtc tgtcatgcat
    15061 tatgaccaat atggaagtgg gtctgtgcta aaatacatag gagatatatt ttttaatttt
    15121 tatggaggtg ttagcagaca taaggggggt ccctgttgga gtgcagggat gcatgctcaa
    15181 aaatatgatt ttggagtagc taagagtcat gctatagaag atatgttaaa tagtaaaagt
    15241 atatttgttt ggggaaaaaa tccagcatat actacaattc atactatgca aataattaaa
    15301 aaagctaaag aaaaagggat aaagatagta gtgattgacc ccatatatac aaaaacagct
    15361 caaatagcag ataagtatgt acaagtaaat cctggaacag atggagcttt agctatagct
    15421 atggcaaaaa ttatagtaga agataaatta tatgatgaag aatatataaa ctcgtatgtt
    15481 attggttttg aagaatataa aaaatattta tcttcattgg aattaagttt tttaattgat
    15541 gaatgtggtg ttaaagagaa tgatataaga gagttagtgg atttatatac taacaaatat
    15601 tcaagtataa atgttggata tgggcttcaa aaatataaaa atggtggaaa cacaataaga
    15661 gcaatagatg ctttaggtgc tataactggt caaataggat ttagtggtgg aggggtaaac
    15721 tatgctaata aagtttatcc gagtgttata aattctgacc cttacaatag ccaatcatat
    15781 ggtgaagata gggagtttta tgtaagtaat atcagtaaat ttatagaaga atctttaaaa
    15841 aatacatcaa ataaagtaaa ttatgcatca gatgaacttg atatgacatc aaataaagta
    15901 aattatgtat cagatgaact tgatataaca tcaaataaaa cagattatat atcaaatgaa
    15961 ttgtataatt tatctaataa gtcaatcaaa gataatattc ctataaagat ggctgtgata
    16021 actaaaagta atatgctgaa tcaactgcca gatttagtag aattggagag ggtgttttca
    16081 aaaattgaat ttaaagtatg ttttgatatg tttataactg atacagctac tttatgtgac
    16141 atgtttatac catgtacaaa tacactagag agtgaagata taatttatag ttcaatgacc
    16201 aatccatata taacctataa tgaaagagct gtaaagccag cacataaatt gatggatgaa
    16261 tattacttct ttatggagct agctaaaaag atgggattaa atgattatcc ttttgtagaa
    16321 aagagaactt atttagaaaa agtgattgaa ccacttaagc gttttgacaa aaatctggat
    16381 atagaaaaac ttaaaaacaa ttactttact atacataacc ctgttgcttg ggaagataag
    16441 aagtttgaga ctccatctgg aaaatacgag ctttattcag aaagtataaa aaacttggga
    16501 ataagtccaa cacctgtata tataagcaat aaatataaag aaatagaaga taaaaatatt
    16561 tcttttagat tattaactaa tcatcatgca gatactttat ttagtcagca ttttatggac
    16621 aaaaaatcta tagctcaagc atatataaat cggagaatgg ctaaaaaagt aggtattgag
    16681 gacaaagata ttgtgatatt gaggtcaaaa aaagcaaaaa taaatgtaca aataaatata
    16741 gatgatggag taggtaatta tatagtaaaa atgtatgttg gttggtggaa gaaacatgga
    16801 aatcctaata gtttaacaga cactggtata tcagattttg ggggtcaggt aacatataat
    16861 gaaagtatgg tagaaataat acgacaaaac tagttaaatt tcattgaagg aataaaaata
    16921 taattataga attgattaaa agttactatt tttacaaaac tattgaagga gggatttttg
    16981 atggtaaatt attccttaga acataagaat ctatacatag aatttatgtg ctcagaacta
    17041 gaccaccatg tagccaatga aataagggaa gaaatagata acttattaag tgtaaatcaa
    17101 gtaaaaaatg ttgtatttaa ttttgaaaat attaatttta tggattcttc aggtataggc
    17161 gtcatcatag gtagatataa aaagatttct aatgaagggg gacgagtttc agtaattaat
    17221 ataagttcac gagttaagaa gatttttgat ttatctgggt taaataaaat tattggcatt
    17281 tatgatacgt atgaagaagc tctaagttct ttgtagggag gagtatgtga tgaataatat
    17341 tatggaagtt aaattttcgg caatatctga aaatgaaagt ttagcaagag ttatagtagc
    17401 atcctttgca gctaagttag acccaacagt agatgagtta gtagatataa aaactgctgt
    17461 ctctgaggct gttacgaatg ctataataca tggatatgaa gaagacagtt ctaagtttgt
    17521 tttcttaaga tgtgagatag aaggaaatac tataaagatt atagttgaag atgaagggta
    17581 tggaatagaa aatgtggaga aggcaatgga accactttat acatcaaaac cagaacttga
    17641 tagatcagga atgggattca cagtaatgaa aagctttatg gacgatgtag aggtaagttc
    17701 tgtaaaagat aatgggacta gaattgaaat gactaaaaaa ataaatgtgc ctaaataaag
    17761 tccaaaagtg aggttttatt atggaagtaa ctgttgccag agaagaaaaa aaacctcttc
    17821 ttagtcatga agagactcta gaacttattg aaaaagttca aaatggagat gaagaggcaa
    17881 aagaaatatt aatatcaagc aatttaggtc tagttaggag cgttgtatct agatttttaa
    17941 atataggata tgatagagaa gatttatttc agttaggttc tataggctta ataaaatcaa
    18001 tatataaatt tgacccaaaa tttaatgtaa aattctctac ttatgcagta ccaatgattt
    18061 taggtgaaat aaagagatac ttaagagatg atggtatgat aaaagtatca aggtcgttaa
    18121 agcaaatagc tgtaaaggca aagatggaaa gtgaagcttt aactaaaaaa ttaggtagag
    18181 agccgagtat agaagaatta gctaaagcaa tagatgtaga aaaagaagat ttagtaatgg
    18241 ctatggaagc taatttcaat gttgagtatt tgcaaggagt tattcatgaa gaggaaggtt
    18301 ctccaatatg ccttattgat aaaataagca tgaaaggaga aagtgaagaa gaaaaagtag
    18361 tggataacat attgttaaaa gacatacttg gaagactgga taagagagag cgtcaaataa
    18421 tagttctcag atactttgaa gacatgactc aaagtgaaat aggagagatg ctcaatatat
    18481 cacaagttca agtatctagg atagagaaga aagtattgtc taagttaaaa gaatatatat
    18541 cgtgataata cttatgatat gtagaataac aaaatataat aaatatatta ctaaaataaa
    18601 atgtaataac tttttatgtt aagtttttaa attattaaaa gatattaaaa taataaaaag
    18661 cattaaaaaa gtaaaaatat aaaaacaagg tgtctttatg gaaaaataga ttttttcttt
    18721 attagacatc ttttttattt tttgaccaaa ctttagatag tcaaaataaa taataggttt
    18781 atttttttaa aattaacaaa taatacttta gagaggtgtt tagtatggat aaaaattata
    18841 aaaaatatgt agaccaaata agcccaaaac ctacatattt aaaaaactat acacttgcat
    18901 ttattgtagg tggaattatt tgtatgatag ggcaagcaat aaatgatttg tatatgaaag
    18961 ttggaggtct tgataaacta ggtgctagtt ctgcaacatc aataacatta atatttatag
    19021 gggcattttt aactggatta ggagtctacg acctaatagg aaaaagagct ggagctggtt
    19081 ctataattcc aataacaggt tttgctaact caatagtatc tccagcaatg gagtataaaa
    19141 gagaaggcta tgtattaggt gtaggtgcaa acttatttaa aattgcagga ccagttttag
    19201 tttatgggat aggttcttct attttatgtg gaattattta ttatattttt aagatgtttt
    19261 agatgatttt tatttaattt aaattttatt aattatgaag aaggtataaa atctgaaaat
    19321 agctactaaa gtaactaatt gaaaaataat tgtgcagatt taattaaaat ttttaattaa
    19381 tattaaaaat ttgaatagta aaatgcaaaa ttaaaaaggt gtgaaaatat gaaaaataaa
    19441 agaattggaa aaagaacagt caagttagaa aataaaccaa ccataatatc aactggaact
    19501 atagtaggtc ctaaggaagg tgaaggacca ctaaaggact attttgatat gattatgact
    19561 gacgatttat atggtgaaaa gacttgggaa ttagctgaaa gtaaaatggt agagactgca
    19621 tctcaacaag ctatccaaaa ggcaggtaaa aaattgtcag atgtaaatta tatgctaggt
    19681 ggcgatttaa taaatcaaat agttccagca tcatttgcag caagagaact ggctattcca
    19741 tttttaggta tatatggagc ttgttctaca atggcagaag gtctttgtat aggttctatg
    19801 cttatagatg gtggttttgc tgatttagta ctgtcaggta catcaagtca ctattgtaca
    19861 gcagaaagac aatttagatt cccacttgaa cttggaaatc aaaaaccaat gacagcccag
    19921 tggacagtta caggagcagg aagtgtatta ttagctccta atggagacgg tccaaaagta
    19981 aagtatgtta ctgttggtaa agtaatagat gagggtattg atgatggtaa taatatggga
    20041 ccagctatgg ctccagcagc aatagatact atctactctt actttgaaga tactaatgat
    20101 gacccaaata gctttgatat aattgctact ggagatttag gtactcttgg aaaacagata
    20161 gcagaagact ttctaaaaga aaaaggtgta gatatatcaa aagtttatac tgactgtggt
    20221 atagagatgt ttaaccttaa agaacaagat gtccactgtg gtggaagtgg atgtgggtgt
    20281 tcagctacag tgtttgcagg atatatatat gataagttga gaaaaaaaga atttaacaaa
    20341 gtaatgcttg tatcaacagg agcactactt tcaccgacaa gtacactaca aaagcagaca
    20401 ataccatgtg ttgcacatgc agtagtaata gtaaatgagt aggaagtgat ataatatgtt
    20461 aatggactat gtaagagtat ttattgtagg tggaattata tgtttaatag cccaagtaat
    20521 gatggattat ttcaaaatgc aaactccata tgttatggta acgtatgtaa caactggtgt
    20581 agttttaact tttctaggac tttatgagcc attagttaag tttgcagcag caggtgcaac
    20641 agttcctata ataggatttg gatattcact tgctacagga gtaataaaat caattcaaag
    20701 tgatggtttt ttaggtattt ttttaggagg tacaactgct acagctggtg gaatagcagc
    20761 agctatattc tttggatata ttatgtcagt agtatttact ccaaaagcaa aaccataaat
    20821 ctttattaaa agaataataa gtttttgaaa taataacttt ttataaagca ggtggatatc
    20881 ttttagattc cacctgtttt ttatataaag aagcgtttac ataattgaag ttgacaatta
    20941 tttacaacat agtgtagtat tatattatca aaataaaatg aagtgaagta ggggataaag
    21001 atggtaacta ttaaagatat tgcaaggaat ctaggtatca gttattcaac tgtttctagg
    21061 tgcttaaaca ataatcctaa tgtatctgaa aaaacaaaaa agaaagtggt agaagaggca
    21121 aatagactag ggttccattt taatgtaaat gcaagaaatt tagcaaaaaa agaaactaac
    21181 aggatagggg taatcttttc aaataatttt aatcatcaag atactagaaa gttttttagt
    21241 gatataatgg atagttctat aaattctatt gagactaata aatacgattt tataatacag
    21301 cctaataata acattagtgg tgatagtaat gtatataaaa tggtaaatgg acaaatggtt
    21361 gacggattag taattgtgtc tcaatcaata aaaaaagaag agtatgattt tttaaaagac
    21421 aataattttc cacatgtatt tatatatttt aaaccatctt ttataggaga agtagataat
    21481 ttcttttggg acgataacgt gtatggaggt tatatagcta ccaagcattt aattgaccat
    21541 ggtcataaag atataataac agtaacttca gatgataaga gttcaaaaat gcatgaagat
    21601 agaaccaagg gatatttgaa tgctatggaa gaagcaaatt taaagacaga agtcattaaa
    21661 tctaaaatgg attttgaatc tcaagttgaa tttttagaaa aatacattga taaaataaaa
    21721 aaagcttcag ctatatttgt acaacaagat gtaccagcga tttctataat acaagagtta
    21781 aaaactactt atggtataaa tgtaccagaa gatatttcca taatagggta taataatatt
    21841 gaattaatat catattttag gtcgcattta tcaacaatag atgacccaag agagcaagtt
    21901 ataaaaaatg gagtagatag tttagtaaat ataataaata aaaagagtac agaacacatt
    21961 ccaagaaaat tatatccaag gttaataata cgaaatagcg ttaaaagaat aaagtagtaa
    22021 gcttttaagt atcaaacgtt tattaaaaat attatagatt atgtgaagtt ttttaacaaa
    22081 taatataaat aaaaattaaa aggagtag
//
LOCUS       DAENCV010000057        22059 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAENCV010000057 DAENCV010000000
VERSION     DAENCV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.058"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            444..1514
                     /locus_tag="KQD82_002218"
     CDS             444..1514
                     /locus_tag="KQD82_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380688.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KQD82_002219"
     CDS             1498..2367
                     /locus_tag="KQD82_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG4380689.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KQD82_002220"
     CDS             2411..3271
                     /locus_tag="KQD82_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG4380690.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KQD82_002221"
     CDS             3302..4606
                     /locus_tag="KQD82_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4380691.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KQD82_002222"
     CDS             4669..5931
                     /locus_tag="KQD82_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG4380692.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKDGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KQD82_002223"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KQD82_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG4380693.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KQD82_002224"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KQD82_002224"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG4380694.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KQD82_002225"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KQD82_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG4380695.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KQD82_002226"
     CDS             13008..13937
                     /locus_tag="KQD82_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG4380696.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14149..15945
                     /locus_tag="KQD82_002227"
     CDS             14149..15945
                     /locus_tag="KQD82_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG4380697.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16085..17866)
                     /locus_tag="KQD82_002228"
     CDS             complement(16085..17866)
                     /locus_tag="KQD82_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG4380698.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17889..19775)
                     /locus_tag="KQD82_002229"
     CDS             complement(17889..19775)
                     /locus_tag="KQD82_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG4380699.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19792..20289)
                     /locus_tag="KQD82_002230"
     CDS             complement(19792..20289)
                     /locus_tag="KQD82_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG4380700.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20607..22058)
                     /gene="cls"
                     /locus_tag="KQD82_002231"
     CDS             complement(20607..22058)
                     /gene="cls"
                     /locus_tag="KQD82_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG4380701.1"
                     /translation="MYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPAL
                     GFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSDIHQHKDMIYA
                     LANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKDDKIGTEIIDI
                     LIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNH
                     RKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYT
                     TKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLKMIQKARKYIY
                     IQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAKIY
                     TYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFEND
                     ILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8479 a         2956 c         3693 g         6931 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa tcaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacctc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa tatagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttcccca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcaggccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagcctatt tggaaaagtt gtgactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatacaa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccgc tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg acaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccacc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaagat
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctagg
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacaaaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac aaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacatac aacaattcac ggtgtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tggactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatataa tgtttattaa
    14041 taagatatat tatataagga taatttaaag tacagtgtta gattataata atactagatt
    14101 aattttatat ttgagtttca ttatataaca agaaagttgg aattttttat gggaatagaa
    14161 tattttagta aacttttaga aagtagtact gaaaaagaat tgctcttaag taaaaagtca
    14221 aataacataa gtacagcaag attaatatct tttttgatag tgatagcagg gtttgctatt
    14281 ggattttaca ataaaaatat agtaggtatt tttgttggag ttttatcaat aataatattt
    14341 atagcactct tagttattca tagtaaagta aaagaagaag aaacatattt taaaagtaag
    14401 agcgaagtat taaataagta tattaagaga tttggagatg gttggaagga atttaaaata
    14461 gatggtaaag aatacttaaa agatgaaaat tcgcaagcaa aagatttaga cttatttgga
    14521 agagcatcat tgtatcaata tatctgtgta gctaatacat cttatgggaa aaatttttta
    14581 gccaaatatt tgtggaatga aaatccagat gagaatgtaa tcttagaaag acagagagct
    14641 ataaaagaac ttttatcaaa agatgatttt tctattcata ttcaaacact cagtaatata
    14701 attggtaaag agcaaaaaaa caattcagat agtagtatag aatcgtttat agagtatgga
    14761 gaaaataaga atgtgtatat accaaaatgg atgcatattt ttacttgggg tttaccaact
    14821 gcaacaatct tatcttttat attttgtatg ttaggatttt tacctgtttt acctgttttt
    14881 ttacttttta taatccagtt agggtttagt ggttttggat atccaagact tatgcaaact
    14941 cttgctccat tattttcatt tagtagaagt atacaagtat atgaaaaaat gtttgaggtt
    15001 ttagaaaaag agacgtttga aagtgcatat ttgaaagaat tacaagataa actttctaag
    15061 ggaagtggag tttcaagagg tgttaaacaa ttaaattcaa taggaaatgc tgtaaaccta
    15121 aggtataatc agatactata tataattgcc tgtggtgttt tgatgtggaa ctatcattgt
    15181 gcagaagctt tggagcgatg gaaaggtata tatggaaatc aaattagaga ttggtttgaa
    15241 tctattggag aatttgaagc tctaataagt ttaacagttg tttctcatgt aaaggaaaat
    15301 acatgttttc cagtaataaa atatgaagat actccaagat tgaaagttga agaggtatac
    15361 cacccattaa tagcagaaaa aagtgtcata gcaaattcga tacaattaaa ttctcagact
    15421 tgtattatca caggttcaaa tatgtctgga aagacaacat ttttaagaag tatcggtgta
    15481 aacttagtgc tggcatatgc aggtgctcca gtatgcgcta agagttttga tgcgacctgt
    15541 atggctatat ttacttctat gagaatacaa gatgatgtaa gtcaaggaat atcaactttc
    15601 tatgctgaga ttcttagaat aaaatcaatg attcaatata gtttaaaaga actgccaatg
    15661 ttggttctag tagatgaaat ttttaaggga actaactctg ctgatagaat tattggagca
    15721 agtgaggcag taaagaaatt atcaaaaccc tgggttataa gtatggtgac aacacatgac
    15781 tttgagctat gtgacctttc aggtagtgga gatgttgaag ttgtaaacta tcatttctca
    15841 gagtactatg tagacgataa aattcatttt gactataaga taaaagatgg aagatgtaaa
    15901 actacaaatg caaaacaact tatgagaatg gcaggtattc tttaattaat agataggtta
    15961 attaaagaat aagtctattt agaaatataa tttaatttgt gaaattaata aggattgtct
    16021 taaattacgt aaaagtattt taagacaatc cttttttaag aataaatttt atactttgtt
    16081 atgactactt agcttcttca caagcacatt cttgtggaca ctctccatag acacaactct
    16141 tatcaaagta atgagtatga aggtatttat gagctacatc actatttggt tctccaaaga
    16201 agtctttata aatagcttgt aaatcaggat tctcatgaga tttacgaata gccttatttc
    16261 tatctatttc atataacgca tttgcacgtc tatcaactat ttcagtatta ccatggtgat
    16321 aaggttgacc agcacctcca acacatccac caggacatgc cataacttct atgatatggt
    16381 atttacattc tccacttctg accttatcca taatctttct agcgttttta agtgtattta
    16441 caatacacac attaactgta gttccatcaa catctatact tgcttcccta attcctttaa
    16501 gacctctaac attagtgaag tttacattat ctaaagtctt attagttatc ttctcatatg
    16561 aagtacgtag ggcagcttct aaaactccac cacttgctcc aaatatagat gcagcacctg
    16621 tagattttcc tagaggatta tcaaaatctt ggtcttgaag attaggtaaa tcgattgcag
    16681 cttcttttat catcttagct aactctctag tagttataga aaggtcaaca tctagtattc
    16741 cagattcact taattcttct cttgaagctt catatttttt agctacacaa ggcattacag
    16801 atacaacata aagttcttca ggattgattc ctaatatttt aggtgcatag tagtttttag
    16861 ctatagaacc aaacatacct tgaggagact tacagctaga tgctaagttt aacttgtctg
    16921 gataattgtg ctctaagaag tttacccatg caggacaaca acttgttaat ataggaagat
    16981 tttcaccttt ttggattctt tctataaatt cagtagcttc ttccattata gtaaaatctg
    17041 caccaaagtt tgtatcaaat acatgttcaa atccaagtgc ttttaaagca gctaccattt
    17101 ttcctgtaga gattgaccct ggttctaatc caaactcttc accaatagca actcttacag
    17161 caggagcaac ttgcactact atagtctttt cttttttatt taaaacatcc caaagtttag
    17221 gtacattatc tacttctgtt aatgctccag ttggacagac agatatacat tgaccacaga
    17281 atgtacactc tgtttcaacc atatcagagt taaagaatgt actaactaaa gtattaaatc
    17341 cacgattaac tccagataat gctcctactg tttgtatgtc attacataca gtctcgcatc
    17401 ttctacataa tatacactta gaatggtctt taactaaaga tttagtagaa gtatctttac
    17461 ctgcaaaaga tttagaacct tgatatctta tttttcttac tcctaaatca gcagctatag
    17521 tctgtaattc acaatctcca tttttttcac agacaaaaca atcttgtgga tggtctgata
    17581 acaataattc aacaatagtt ctacgagcat ttaatgcttt agcactatta gtctgaactt
    17641 tcataccttc ctttataact gttccacaag ctggaactaa tcctctctct gtctcaacca
    17701 tacaaacacg acaagaagca caagtgtcaa gcttatctat ttcattcata tgtaaatgac
    17761 aaaggttgtg tatttttata tttattaatt tcgcagcatc taagatagaa gttccactag
    17821 gtgctgaaac gtgtttgcca ttaatagtta aattaactaa actcattgtt attcctcctc
    17881 caaataagtt atttcttgat tatagcgtta aatgtacaat tatctataca agcaccacat
    17941 tttagacact tagaagtatc tattgtatgt tgttccttaa cagaacctgt tatacaacca
    18001 gcaggacaca ctctagaaca ttttgtacaa cccttacagt catctgttat aaagtaagaa
    18061 agtaagttct gacatttacc agcaggacat ttcttatcta caacatgagc tatatactca
    18121 tctttaaaat attgaagagt acttaaaaca gggtttggag cagctttacc aagaccacat
    18181 aatgaagcag tttgtatagt ttcagcaagg ttttctaggt catctaaatc ttgtagagta
    18241 ccattacctt ctgtaatttt agttaatatt tctaataatc ttgtagtacc tatacgacaa
    18301 ggtgtacatt taccacaaga ttcttcaaca gtaaactcaa ggaagaactt ggctatatct
    18361 accatacagt cagattcatc aagaacaagc ataccaccag agcccatcat tgaaccgata
    18421 gaacttaatg aaccaaagtc tataggagtg tctaaatgtt cagttggtat acatccacca
    18481 gatggtccac ctgtttgaac agccttaaat ttcttacctt cagggattcc cccaccaatt
    18541 tcaaatacaa tatctcttaa agtagtaccc attggaactt caacaagtcc tacattttct
    18601 acttttccac caagagcaaa gactttagta ccactagaat cttcagtacc taagttggca
    18661 aaccaatctc cacccttaag tataattgca ggtatgttag caaaagtctc aacgttattt
    18721 aagcaagtag gagatttcca tagaccactc ttagaagaac tataagtttt cattctaggt
    18781 tcaccacgtc taccttcaat tgaatgcatt agcgctgtac cttcaccaca aacgaaagca
    18841 cctgcaccat attttaattc taatttaaag ttaaatccag tgcctaagat attttcgcca
    18901 agtaaaccgt atttttcagc ttgagctatg gctactttta atctttctat agattttgga
    18961 tattctgctc ttatgtatat gtatcctgta tcagaaccta tagcgtatcc acatattgcc
    19021 atagcttcta gtacactatg agggtcacct tcaagtacag atctatccat aaatgcacct
    19081 gggtcacctt catcagcatt acatacaaca aactttttat ctgttggacc agctggattt
    19141 tttgaagcag cttcccattt agagccagta gggaaacctg caccacctct accacgaagg
    19201 ccagaagttt ttacctctgc aataacttct tgaggagtaa gagaagttaa acatttgcct
    19261 agagctaaat aaccatcatt ggcaatatat tcttctatac tatctgggtc tataagacca
    19321 caatttttta aagctatacg taactgtttt ttgtagaaag acatttcttc ttgttcttca
    19381 acttttttac ttaaagattc ttcctcatat aataattcct cgacaactgt atttctaatt
    19441 aagtggcttt gaactatctt ttcagcatca gatggctcaa cttttacgta gaaaacgtta
    19501 tcaggataaa cttttacaat aggaccttga gcacagaaac caaaacaacc tgtcaaacga
    19561 acactaactt tatcttggat tcctgatttc tttatttcat tttctaattc actaactatt
    19621 tctagactgt ttgaagatgt acatccagta tcacaacaaa caagtaattc tcttcttaat
    19681 acattttcct tagaataatt ttttcttaag ctaagattag gtttcagctc atcagctaaa
    19741 acttttaatt catcaaatga atttacttta cgcattattt attcacccct cttaacaatt
    19801 caattctaat tctctataag tatctaatat ttcgcttact tggtcaggtt tcactttacc
    19861 ataaacttta ccatttacag taacaactgg agctaaacca catgccccca aacatcttaa
    19921 accttctaaa gaaaatttaa aatcactagt agtttctcca acttttatgc caagctgttt
    19981 ttcaaaagca cttaagattt tgtcagcacc ttttacaaaa catacagttc ctagacagac
    20041 actgatttta tacttaccta caggttctgt agtaaaataa gaatagaatg taacaacccc
    20101 atataccttt gcaggagcta ctccaagttt tctagcaaca tgaagttgaa cttcttttgg
    20161 aagatatcca aatatacctt gggcttcatg tagaacttgt attaacgcac cttcctttgt
    20221 tgcaagagag tcaataaata tatctagttc gtcaaataat tgcttgttgt gtgaaataaa
    20281 atcgcacata acaaatcccc cttttctttt taaagttatt atttttcata aaataattaa
    20341 ccatgagttg accccaacat tatgtgggtg aacaaaacca aaacaataaa gaaagaataa
    20401 tatacaacaa tttctttatt tataaaaaca atgcaaaaaa gttacctatt aaaaataaaa
    20461 actatactag tctattataa tataaaataa ttcagaatag ttaaaaaaaa taaaaaaaat
    20521 gagctaaatt caataaaaaa aaaataatac atataaaaaa ctttaaaaaa taaaccaaaa
    20581 tgtaaaattt ttataaaata aaagatttat aaaactgctg ataatagtct tgaaattgat
    20641 tccttgattt ttatataagt agacctagaa ttatatatat ctaatgttat ttccttagaa
    20701 tttaaaatat cattttcaaa tataattctt tgttccaatg ctttttttga agagtacata
    20761 aatgcattaa cttcgaaatt aagctcaaaa cttctgatat ccatattagc tgttcctata
    20821 gagcatatag agtcatctat aactattgtc ttagcatgta aaaatgcatt ttgaccataa
    20881 gtatagattt tagctccaaa ctttaaaagt tctccagcat atgaatatga tgcccagtat
    20941 acaaagggat ggtcaggttt agaaggaatc atcactctca catcaacacc agataaacaa
    21001 gctattttta aagtatctat aaaagttgag tcaagtatta aataaggact ctgtatgtat
    21061 atatactttc tagctttctg aatcatttta aggtaaccat atttaatctc atcaagttct
    21121 gatatatctg gaccacttga tactatctga atcccaatat tttcagaagg atgattagaa
    21181 tttgtatctt cttctataaa gtacttttct aaatctaaat cttctttagt tgtatatctc
    21241 caatcaagga tgaatctcat atttaggtca attacgcaat caccagtaag cttagtatgt
    21301 gtatctctcc atagaccaaa ttttggattt ctacctaaat attcatcacc aacattatat
    21361 ccacctacaa atcctacctt accatcaatg acaacaattt ttcggtgatt tctatagttt
    21421 aagttaaagt ttacaatttt caagaatgat gggaagaagc ttccaacttt aacaccactt
    21481 tctttaagtc tggaaagtgt actatttttt agtgttctac caccaactgc atcaaataaa
    21541 agtcttactt caacaccttc tttagctttg tctataagaa tgtctattat ttcagttccg
    21601 attttgtcat ctttgaaaat gtaaaattgt atgtttatat attttttagc ttttttaagt
    21661 tcttcaagaa gactgttaaa aaattgacta gattcagcat aaatccacac atcgttatta
    21721 ttggtataat gagcattatt tgaattagcc aatgcataaa tcatatcctt atgttgatgt
    21781 atatctgaat caatttcaga tgtagattgg agtttaacct gagtatctag tatattactt
    21841 ttgattatct tatcatcctt ttctttcaat ctaaacatat tgttttttga aatatttctg
    21901 ccaaaagcta tataaagtat gaatccaaga gcaggaatca atactaatat aagaagccat
    21961 gttaaagttg tatttatgtt tcttttttct ctaaagataa ggtttagtat aaccatgaaa
    22021 ttaattatat atattgtagt aactgctatt tcatatatt
//
LOCUS       DAENCV010000058        21937 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAENCV010000058 DAENCV010000000
VERSION     DAENCV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.059"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            119..355
                     /locus_tag="KQD82_002232"
     CDS             119..355
                     /locus_tag="KQD82_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380702.1"
                     /translation="MNNMENKKNFLRKIVKLRSIFLSIFGVFLFITATTGIQTVDRYV
                     PTWLYITLGVSATISLVLYVISFILKINIKDSKM"
     gene            complement(1117..1317)
                     /locus_tag="KQD82_002233"
     CDS             complement(1117..1317)
                     /locus_tag="KQD82_002233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380703.1"
                     /translation="MSERNSYSKTDNDATFMRMKEDHMKNGQLKPAYNIQFGVDSEYI
                     VLVSSGHQPTDTITLIPFLKSI"
     gene            complement(2012..3331)
                     /locus_tag="KQD82_002234"
     CDS             complement(2012..3331)
                     /locus_tag="KQD82_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4380704.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(3372..4118)
                     /locus_tag="KQD82_002235"
     CDS             complement(3372..4118)
                     /locus_tag="KQD82_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG4380705.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(4362..6371)
                     /locus_tag="KQD82_002236"
     CDS             complement(4362..6371)
                     /locus_tag="KQD82_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380706.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEAMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(6809..8242)
                     /locus_tag="KQD82_002237"
     CDS             complement(6809..8242)
                     /locus_tag="KQD82_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG4380707.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDILQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(8258..9682)
                     /gene="ascB"
                     /locus_tag="KQD82_002238"
     CDS             complement(8258..9682)
                     /gene="ascB"
                     /locus_tag="KQD82_002238"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG4380708.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFVEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYNRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(9769..11685)
                     /locus_tag="KQD82_002239"
     CDS             complement(9769..11685)
                     /locus_tag="KQD82_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4380709.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(11759..12619)
                     /locus_tag="KQD82_002240"
     CDS             complement(11759..12619)
                     /locus_tag="KQD82_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4380710.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(12970..14157)
                     /locus_tag="KQD82_002241"
     CDS             complement(12970..14157)
                     /locus_tag="KQD82_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380711.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(14171..15562)
                     /locus_tag="KQD82_002242"
     CDS             complement(14171..15562)
                     /locus_tag="KQD82_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG4380712.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(16157..17857)
                     /locus_tag="KQD82_002243"
     CDS             complement(16157..17857)
                     /locus_tag="KQD82_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4380713.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYLIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(17829..19232)
                     /locus_tag="KQD82_002244"
     CDS             complement(17829..19232)
                     /locus_tag="KQD82_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380714.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(19496..19987)
                     /locus_tag="KQD82_002245"
     CDS             complement(19496..19987)
                     /locus_tag="KQD82_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4380715.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(20013..20825)
                     /locus_tag="KQD82_002246"
     CDS             complement(20013..20825)
                     /locus_tag="KQD82_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4380716.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(20838..21797)
                     /locus_tag="KQD82_002247"
                     /pseudo
     CDS             complement(20838..21797)
                     /locus_tag="KQD82_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6941 a         4103 c         2273 g         8620 t
ORIGIN      
        1 aaacaaaaca gtagctaggc tttaaataaa atattaaata ctataattta tagattaaat
       61 ctgatattat tatatacatc tggaagttgt tgctattatt taaaaaggtg ggatggatat
      121 gaataacatg gaaaataaga aaaatttttt aagaaaaata gtaaaattaa gaagcatatt
      181 tctaagcata tttggtgtgt ttttatttat tacagcaact acaggtattc aaactgttga
      241 tagatatgtt ccaacatggt tatatataac attgggagtg tcagcaacta tatcattagt
      301 tttatacgtt atttccttta ttttaaaaat aaatataaaa gatagtaaaa tgtagtactt
      361 cataaacttc cagtaaaaga ataattttga taaatcaaaa ttattctttt ttaatatcaa
      421 aataaataat aaaataacaa aataaataga aaatataact aaaaaattat caaagtttaa
      481 gatgataatt ttatatttga taaacttatg taatacattg taaattaact tctcaaaatt
      541 tataattctt agcataaaat tataatttaa tattaaaatt ttacaaaaaa gtaatttatt
      601 aaatgactag gataatcaat aaaactttat aatttccaca aaagttatga tgaaattgtt
      661 gatttagata taaatttatc agtaaaacaa attagtaagt taaaaaacga tcttataaaa
      721 agattaaaat aaatcctttt attggaattt taagagattt tgtttttatt ataaataaaa
      781 aatttattgg tttattattt aaaaacgtta caagtctttt aaagctcttt aaaacagcct
      841 tttaaattac tttacttaaa tatttttttg tttaacttat ttttataagt aataaacata
      901 taaacatcac ttatgtaaat taatgatttt taaaataaag atatttttat tttttatata
      961 aacagtacag aataaaaaaa tatttgtgtt ttccctttta ctattctatt ttaaaaatat
     1021 tttatttatt ttatatttct ctaaaaatgt atagttttca tcactttcat acccagaatc
     1081 atccactatt ttaggatatt taaactttgt atggaactaa atagatttta aaaatggtat
     1141 taatgttata gtatcagtgg gttgatgtcc tgaacttacc aaaactatat attctgaatc
     1201 tacaccaaat tgtatattat atgctggttt caattgccca tttttcatat gatcttcttt
     1261 cattctcata aaagttgcgt cattatctgt tttagaataa ctatttcgtt cactcattat
     1321 atgaaggtct ttagtatatt ttttcaatct atcaatacaa tcatttaatt gctctataga
     1381 tttgttcttt ccctttccat gaacaaatgt tatatttgat tcttttgata ttttttaacc
     1441 ttctttaaaa atttccttaa atgatggatt ttaattttat ctttataaat tattttaata
     1501 ccaaaatcaa tctcacattt taatatgaag tcagatatat tattcatcat tttagccata
     1561 tttttattta tagctttttt caaacaaaag tatatctatt agcagatgac tctatttctc
     1621 ttgaagaata aatacagtta atgtttgtat atattataat tttcaaaagt tgtctagggg
     1681 ttactctatt tcctctaatt cgagcataag tcttatataa tttttctaaa ttcatctact
     1741 caataatttg acttaataac cttacagaat catcttttgg aatcatgtaa tcaatactta
     1801 gtggtaattt aaattgataa ttatcgccat aagtagtata attaaactat ataccatttt
     1861 gttttaacac acaattatta tacaagactc ccaaactctt ttgaacttgg gattcttgta
     1921 taataaaggg tgtagcacta ttttctagtg atacagcccc atttccctaa attcaaagta
     1981 atatttatag aatctctata gattacaata attatttgac ttctacatca ttatatttct
     2041 ttgaatatac tttagtcata tagaaatata taggaactac aattatcatc ccacctaatc
     2101 cccaaactat cttcataggt tcatcaatac ctgcttttat cagtaataat atacttataa
     2161 taactgcaaa tattggtata attgctcctc caggtattct aaaatttaca tctttaccag
     2221 catacttctt tctcaatatt agtacagcta gacatgtagg aatatattga ataaatctag
     2281 atacaacact tatttgagat aatgttgtga atgaacccga ccatgctatt agtaaagttc
     2341 ctatgacaga tactataatt gctatataag gagctccatt tttattagtt tttcttatag
     2401 acttaggcat taatccttgt tcaaccaaag cagcagcaga ccttggtgta aatatggatg
     2461 atgcaatatt tatacctcca acagatacta tagttcctat tgtaattata tacccgccta
     2521 taggacctaa catcttaatg gcagcatctg caacaggagt agctgaccct gctaagccat
     2581 tacccaacac ccctatactt attcccagta taagcatata tattactgat actacaaaca
     2641 ttaccattac caatgcttta ggtacatttt tcttagggtt ctccatatct tttgctgcaa
     2701 cagctaaaga ttcaaatcct gtaaaagcat aaaataaagt tatagatact gctacaaaag
     2761 ctgatgacgc actttgacct ttttgtatta ttacaaatgg ggtaaaatta gaacccttta
     2821 taaagaataa tcctacagct ataaataaaa ctattggtaa taattttcct atagtcatga
     2881 tattatttaa gtgcttagat tgtttcattc ccataaaatt tataatccca agccctacaa
     2941 ctaatatacc aattattata tttttagtaa gtggttctgc tgcttgtggc catacagatg
     3001 atagtgttac agcaaagaaa tttgccatag tagcccaagc aatcatacta actatccact
     3061 tcataaatcc aacttcaaat ccaacaaaat ctccaaatgc ctcttttgca taaagatagg
     3121 gccctccatc agtatcaaac caactacctg cttcagcaaa acataaagca atagaaacag
     3181 ctaaaagcat acaaaaaact aatactaata tacttgctgg acctatatct gtataagcat
     3241 caccaggtaa cccgaatata cctgacccta ttattgcatt gattcctaat agtacaatac
     3301 tccaaaaacc taacttaatg cctttttcca ttgttattcc ccccaaagcc aacttaatat
     3361 atttatattt tttatttaga tgcttcttta actattgcca taaatatttt tttcatatta
     3421 tcataatccc tagtcatcat ttctggatgc cattgtatcc ctattacaaa ctcacttcct
     3481 tccttttcta tagcttcaat taaaccgtct ttagatgtag ctgaaacaat atatcccggt
     3541 gctactttgt tgacaactaa gtgatgaaaa ctattaacta aaacttcttt ttctcctaat
     3601 atttcatata attttgtccc ctcctttatc tgtactgtat gtgtaggtac attagataga
     3661 tgttgctgat tgtgtttaat ataagcacct tcaattaaag ataaatcttg atacaaacta
     3721 cctccttcag ttacatttat aatttgttct cctctacata tacctaaaac tggttttttc
     3781 atatctaaag catgttttaa taatttcata tcaaaactat ctctctttgg tgatattgcc
     3841 cccaacttat tatgaggttc ttctttccat atgagtggat ttacatcttg tcccccagat
     3901 agtataagcg catctatatt agatacctgt tcctttataa tctcatcatc atatacaatc
     3961 ggtacaatat atggtatagc tttacacata actactgatt gtatataatc attatttaca
     4021 taagcccttt catagccagg aaacattcct ccttcatcaa ttagtaaatt acctgatatt
     4081 cctattattg gatatttctt tttagccaat ttcttcaccc cttaacttat ttgtgaacaa
     4141 taaaaaatgc tacttctctt aaagagcaag tagcatacca agattttatt tatttgaaca
     4201 ttataattta ttttagaaat actttttttg aatacgagta atataaataa ctttatttac
     4261 ttatatttta ttttaatcct gtttagtttg acttttttaa attgttttaa gtacaactaa
     4321 taggtaaaat aggcatttag attttccttt ttatcaacct attaaatact gtttaatata
     4381 ttttttcccc ttctatttaa ttcacttccc tttctaccag attcacatat tatcaaatcc
     4441 agctctttta acatattaag tattcccctt acctcacctt ctgttatatt gtagcctaat
     4501 tcatttattg aattaacaat accatttcta cctatatttt tattaagatt attggcattt
     4561 tttaaacact ccattactaa ctttactttc tctatagatg ttctatcctt taatatactt
     4621 atttctttac taaaatcttc agttatattt aataagttat gaggtaaatc atttattaaa
     4681 ataacatcct cagaactcat aatgttaata tacatagcag tattttttaa ttctcttata
     4741 ttacctggcc attcatatgc attaataatg tcttgaacat cttgactaat tgctctcttc
     4801 cttttcataa aatgctttaa catagcttct atatcttctt ttcgctctct taaaggtggt
     4861 atatttatgg gcaatacatt tatcctatag tataaatctg ctctaaatct atctcctttt
     4921 atcatttcta ataaattttt atttgtagcc gcaattactc taacatctat atctattaca
     4981 ttttctcctc caaccctcat tacttgattc tcttgtaata ctctaagtaa tttgctttgt
     5041 aagtatatag gcatgtctcc tatttcatcc aaaaatatag ttccattgtt agcctgttca
     5101 aataatcctt ttttacctcc ttttaaagct cctgtaaaag caccttcttc atatccaaaa
     5161 agttcacttt caagtaaatt ttctggcata gctgcacagt ttatcgcaat aaatggctga
     5221 ctatgtcttg gtgatgcatt atgaatagac tgagccatga gttcttttcc cgttccactt
     5281 tctcccgtta taagaattgt ataatcagat tcagctattt tcatacctaa tttcttagtt
     5341 ctttccatct tcgttgaaac tgtttttata tcatcaaaag tatatctagc aacttgtccc
     5401 ttttctctaa gcttctttga taggttttgc tccaattttc ttatgtaagt tatttcttga
     5461 atacaaaaat aatatccagt aacccttcca aaactctcta tattatactt atttacgttt
     5521 aagtacttat tattaaattt tataacttca tcatgtgcct tactcaaact aaaaatcttt
     5581 ttaatattct cattctctaa ttcatctata tttttcccta taatattact ttgaatatct
     5641 agtatatccc ctaaactatt attgcaaata attatgtttc ccttatctga taccattgcc
     5701 atacctaagt ttgataaatt tattatggaa tttagttcat ctgcttttat agtaagttct
     5761 ttgtatttag tatttattcc tgaatataaa ctaattattt catctgtata cttaattagc
     5821 ctttcagtaa tttctttatc atccagtttg agttttgcta ttatttgaat aaacgttgaa
     5881 ctatcaatat acctatggcc taaatcaata acttcttcta tatattttgg aactaaactt
     5941 gcctctcctg gcgttatagc tatcttaata tttctatatt cttcattggg attgtatggt
     6001 ataaaattca gatgatttat tcctatattg tataaattag atattgtttc caatacagta
     6061 tgcttattat cgtttactac taatacatct attccttctg gcaatgaaaa taacttatat
     6121 atatcctttt gctttatact cctatttata gttattatct ttgagacatc ttctacatat
     6181 ttttttattt ttaatactct atcatctatc attaccaata ttatgtcatc ttttattaat
     6241 tccttctctg ttattttatt taaataataa ttttttatca ttactttatc tttaaatatc
     6301 atttctagat ttttatttaa gaaacattct aatttagaca acccatcagt tactactgct
     6361 attgttttca tttgattact ccttttaaaa tatactctac tcttttataa tatacatctg
     6421 attttcactt taacagcaca aaaaattgta catttagtat actaacatat tcctttatta
     6481 gttttatgtc cctaaatatc atagttgcaa tatctatcat atttcctaaa cttctataga
     6541 gcaaaaattg tgccaaatta ataattttct taattttatt ctaaagttcc tcaatagtat
     6601 ttacagactt agcacttata gcttataagt ctttatatta ttaatctatt tttataggtt
     6661 aattgtacaa atcatatatc acttttaccc attaaatgca ttataaaaaa gatggaataa
     6721 cgattcgtta ctccaccttt tttgtaataa aaaattgtat atataaacac tatttactac
     6781 tcttctaagt acaaacagta tctacatact ataacctttc accattagat tctattactt
     6841 gtttatacca gtcaaaactc tttttcttaa tcctagacaa gtctccactt ccatcatcat
     6901 acttatccac gtatataaac ccatatctct tggccatctc accagtagat gcacttacta
     6961 aatctataca tccccatgga gtatatccaa ataaatcaac accatcttct acagcttcct
     7021 tcatttgctc tatatgtgct tttaaatact ctatacgata gtcatcattt atcactccat
     7081 tagattcttt tttatcataa gctcctaaac cattttcaac taccatcata ggtattctat
     7141 atctatcata aatttcattt agtgcatatc taagcccttc agggtctatt tgccatcccc
     7201 attcactaga cttaagatat ggatttttca ctccacctaa tatgttgcca cttgtattat
     7261 cttctttaga atcactactt acacatgtag acatataata actaaatgta taaaaatcta
     7321 cacaaccttc ttttaaaatt tctaaatctc ctttttccat ttttatttca atattatttt
     7381 ctccaaaata tctattcata taagttggat attcgccacg cacttgtaca tctccacaga
     7441 aataattcat tactctacta tctctttgag ttttaactgc atcatctgga ttacaagtat
     7501 aaggatacat tgttgcaaat atttgcatac atccaacttg ataatttgaa tctatttcat
     7561 gagctaactt aacagcctta gcacttgcta caaattgatg atgtaaccct tgaaaacgaa
     7621 gttgaagtat atctacttgg tttataaaat ctgtagtccc ttcatttaat attccttgtg
     7681 ataagtagcc tcccataggc attgtagctg aatttatttc attaaatgtc agccaatatt
     7741 ttacttttcc tttatatctt ttaaatattg tttcacagta attcatatac aagtctatta
     7801 catctcttga aacccatcca ttgtatttct ctgttattgc aaatggtatt tcatagtgag
     7861 atattgttat taatggttct atattatatt ttaaacattc atctatcaca ttttcataaa
     7921 atttaagtcc tgcttcattt ggttctttct ccattccagt tggaaatatt ctagtccatg
     7981 ctattgaaaa tctaaatacc ttaaatccca tttctgcaaa taaagctata tcttctttgt
     8041 atctgtgata aaaatctatt gcttcatgac ttggataaaa tgttcctttt tctataactc
     8101 tcgttattct tttacttact ttttgagaac ctccagtaca catatctgaa caagaaggac
     8161 ctttcccatc taaattccat gctccttcac attgatttgc tgctgttgct ccaccccata
     8221 aaaaatcttt tttaaatgac atacctatat cctcctatta taattcttca ccatttgatt
     8281 ctattacttt tttataccag gcaaaactct tctttttact gcgttttaaa gttccatttc
     8341 catcattatc tttatccaca tatataaatc catagcgctt cttcatttct ccagtacttg
     8401 cacttactaa gtcaatacat ccccatggtg tgtaccccca taaatcaaca ccatctaact
     8461 caacagcatc tatcatggct tttatatgag cacgtaaata atcaattcta taatcatcat
     8521 ttatatttcc attttcatct ggattatcta ctgctcctag tccattttca actataaata
     8581 atggtttttg ataacggtta tataaagagt tcatagttat tctaagccct aatgggtcta
     8641 tttgccatcc ccactcactt gcatctagat atggattttt aagacttgca aaaacatttc
     8701 cttctgttaa aatatttact tttgggtctg cacttgtaag tcttgatgag taatatgaga
     8761 acgaaataaa atctactgtg ttctctttaa gtatcttttt atcatcttct tccatcacta
     8821 tatttattcc ttttctctcc aattctttta atgcatatgt tggatattct ccacgagatt
     8881 gtacatctat aaaaaagtaa ttttccctat ctttttctat agatttccat acatctagtg
     8941 ggttacaagt attaggataa gtatttcctg ctgctaacat acaccctatt ttaaattctt
     9001 gatttatgtt atgtgctagt ttagtaacta acgcactcgc aactaattga tgatgtgcag
     9061 cttggtattt tatttgttct tcattttctc cctcttcaat atagagacct gcacctataa
     9121 atggtaaatg aagtatcatg tttatttcat taaatgttag ccaatacttt accttacctt
     9181 taaaccttgt aaaaataact tcacagtatt ttacatataa gtctactagc ttacgatttc
     9241 tccatgctcc atattttttt accaaataca tgggaacatc aaaatgacag attgttacta
     9301 ctggttctat tccatacttt aaacactcat caaataaatc ttcatagaat tttagtccct
     9361 cttcatttgg aacatcctca actcctgttg gaaatatcct agtccatgct attgacatac
     9421 gatagcattt aaatcccatc tcaacaaata attttatatc ttctttataa ttgtgataca
     9481 tatctattgc ttcatgagct gggtaatagt gttcatcatc acattcaaac attttcattt
     9541 tcccagccaa aactggaaat ctatcttttc cttgcggtgt aacgtcaaca ttagctagac
     9601 ctctaccatc ttctttatat ccaccttcac attgatttgc tgctgttgct ccaccccata
     9661 aaaaatcttt tctaagtgcc ataattctac ctccaataat ttatttatag taactttatt
     9721 ttaaaataaa aatacatata tataattctt aattaataga actctctttt acgctaaaac
     9781 agttatcaat ttctgactat tatttacatg cttttgtgta ttttctacaa tatccacata
     9841 atcagaataa ttagtaacaa ctactggtgt ttctaagcaa tatccttctt ttgaaatact
     9901 ttctatgtca aaagttatta atgtctgacc ttttttaatc ttatcccctt gattaacaaa
     9961 agatttgaaa tatttacctt ccaactgaat agtatttaac cctatatgta tcaatagttc
    10021 acaaccatta tcagatataa ttcctattgc atgttttgtt ggaaataaag tcattacagt
    10081 cccatcaaat ggtgccacta cttttccttc tttaggtgtt attgctacac cttttcccaa
    10141 tatacctgtt gaaaacgctg catcttgtat ctgctccaaa gcctttactt caccttttat
    10201 tgggctatat actacttctc ttttgttttt ttctataacg ccttgcccat tatcatcttt
    10261 agaaatttct actatagcat catctttcca aaaaaataat gttaaaacaa atcctactac
    10321 aatagctact attattgcta ttatagttgc ataaacacct ttcatatctc cagttgttgg
    10381 atttatataa ttaggtatac caaatattcc aagtccaccc attacataac tttttacatt
    10441 cattatcccc attacagctc ctgccacacc accacctaca cagctatata tgaatggtgt
    10501 ttttcttggt aaagttattc catatattgc aggctctgtt acaccacata tccctgaaat
    10561 aactgcaggt atacacagct cttttatctt cttatctttt aatttaaagt acattgctgc
    10621 tactactgct gtttgtgcaa aagatggtac aaatgctccc actaatattg tgtcaaaccc
    10681 catagtcata aggtttgaca tagctattgg tactaatccc caatgtagac caaatattac
    10741 aagcacttgc cacaataaac cagttaatgc accacataaa attggtgaaa agttaaataa
    10801 agctaaaaat ccatttccaa ctatattaga agcataggta gctataggcc ctataaccat
    10861 aaatcccaat ggtaaagaaa ctaataaaac tgccattggt acaaagaaat tctttactac
    10921 atctggtatt attttagcaa acactttctc caacttagct gctaataata ctattaaaat
    10981 tactggtact actgttgaag tgtagttcat tgctattact ggtaatccta agaaagttat
    11041 atatactggt gattcaaata ttgttccagt aaatagattg tataaaggtt gacctgtaga
    11101 tgctagagta tctaattgaa tacctgaata gcataaaact gcacctataa gcattccaat
    11161 aaatggtttt aatttgaatt tcttagcagc tgtatatcca agaattattg gcatatactg
    11221 gaacattgca tctccaattg catttaacat aatatatgca ccatcagttt ctccatacaa
    11281 acccaatgct gtaaatactg cattaaatcc ttttatcata ccagcagcac acagaactcc
    11341 taatattggt tgaaaaactc ctgatattat gtctataaac ttattaagta atccttttgg
    11401 cgcatctgaa tctgaattat ctattgtatc tcctcctaat ccagccaaat cgcacacaac
    11461 ttcataaaca tctggaacat gattaccaat aactacttgg tattgacctg cacttttcat
    11521 tacagtaact ataccatcca ttttttttag cacatcatca tttgctattg actcatcttt
    11581 taatttaaaa cgtagcctcg ttatacagtg agataaactg ctaatattat cctttcctcc
    11641 tacattcgca atgatttctt ttgatagttg ttcatacttt ctcatttttt ctcctccatt
    11701 ttctttttga aatatgaagg tttggccaac ttaatgtcac cacccaatat atataaacct
    11761 aattattagg atttatatct gacttaatac ttttacttac cactctttca acgtgaatag
    11821 ttaagtataa tttttcctca tctgttaaat cgtagttgta ttttttatgt ataaattcat
    11881 ccaacttttc tatgaattta taagcatttt tatatttaac ttttattaca tttaataaat
    11941 catcttcatt attttcatgt aaattattac taacaattcg ttctgcaaaa tactttaagt
    12001 gagtaataaa tctatgataa taaactgaat cttcatcaaa tactattctg taatggtatt
    12061 taaccatatt tgaaatttct tgtattattt gtgtaatctc atatatagtt tttatacttt
    12121 gatccatttg tgcattaact atatgcagtg ctataaatcc agcttcatct tctgatagtc
    12181 taatttcaaa ttttttttct atataatcca atgcctctaa ccctatttta aattcactct
    12241 tataaaatct ttttatatcc caaagtaata cattttttac acttactcct tcacgagctc
    12301 tttctattgc agtatacata tggtctgtta aagaaatata aatactatca tttaatcttt
    12361 tccctagttt ttctttagca tataaaatta tttcatctga taattccata tatcttatag
    12421 gaatatctgc tattagttct ttaaatttat cagatatatt tgggtctgaa agtttgaata
    12481 tcttatcaat tttatccttt gatatatgct ctcctgtacg ttttttataa gctaaacctc
    12541 tccccataac tactatttcc ttatgatttt catcagttgt aataattaca ttattattga
    12601 gtattttttc aataatcatt gtgatgcccc ctttattagc attaagttaa aataaatctt
    12661 ttttaattat ttagaatatt taaaatgtaa tttattacat aaaaaaaaac ctatttatta
    12721 cacattaaat agactcccat gagtcctgag taataaatag gtttagcttg tatttaaaca
    12781 atcactatcc gtttgtattt tattttaatt attatttatt atatgttagc aaatttattt
    12841 tttagtgtca actttttctc aatattttaa ttttaaataa attatataaa ttgaatcttc
    12901 aaagtactat ttttatcaat aaacaaaata aaaaaggtat tactttcttt aagtaatacc
    12961 catttatttt taatttaagt ggtttttctt aatctcatca agtagtccat cttctaaata
    13021 aatttctaat gcacatccta ccattgcctt tactgctttg tgaagtttat cataagcttc
    13081 ctcagaattt gcataattta gaaattcttt ttcatgaaca tataccattg gacttatatc
    13141 taaggctatt tctgtataca tagtagggca aacttgactt acattaccta tatctgtaga
    13201 acctactgga ccatctttac cttctaatat atctgttata cctgcctcaa tcaaattatt
    13261 ctttgttatc ttttgaagta ctttgttatt tattatgttg tcaaaagaat tttcaaagta
    13321 tctataagaa agcttagctc ctgtcattaa ctctgcacct tttgcacagt ttataacttt
    13381 tttagttact tcatctaaat aacttctttc agcagctctt acgaaaaact tacaggctgc
    13441 tttttctgga actatatttg gagcctctcc tccatttgaa actataccat gtattctaac
    13501 atctgatttt acatgttgtc ttagtgcatt tatcccagca aaagtaagtt gtactgcatc
    13561 tagtgcattg actcccatat gagggtgact agctgcatgt gctgataccc cttcaaaact
    13621 aaactctaat gaatctatcg ccaaagcctt acagtttata ttattaaaag cttctatatg
    13681 catttgataa cttacatcaa catcatcaaa agctcctgcc ttaactaaat cacattttcc
    13741 tcctgttgtt tcttcagctg gtgttcctat tagtacaatt tttcctttaa aattttcttt
    13801 taacttactt aatactatac aagctcctag tgtagatgct gcaatccagt tatgtccaca
    13861 agcatgagct ggttctttat tttctccata tccaggaagt gcatcatact cagctaaaaa
    13921 tgctatggta ggtccatcat tatctcctaa ttcagctcta aaagcagtct ccatattaca
    13981 atatggataa actgtatcaa atccatattc cttcattttt tctactaagt attttgatga
    14041 tatgtattct tcttccccta attcaggatt gttaaatata aaattacaaa cttcttcaat
    14101 ttcttttcca agttcattct ctatctcata agctctcttc tttatactct ctatcataat
    14161 ttacctccta ctagaatcct agatataatc ctattgtcaa aaatgccatt acaagaattg
    14221 ttaatactac cattaaagga attacgaatc taacccactt tgaccaagga atattggcta
    14281 ctgctagtgc tcccattaaa acacctgatg taggtgttat taagttagtt acacctatac
    14341 caaattgata agctgtaact atcatttgtc ttgatacatc tactaagtcg ccaagtggtg
    14401 caagtacagg tattgtaagt gatgcaagcc ctgatgctga tggtattaaa aatgctatta
    14461 atccttctat taagaaagtt aaatttataa atatagtttt tggtaatcca tttaataaat
    14521 ttgctgcaga atttaaaatt gtatctatta taaatccatt ttctgctatt attacgattc
    14581 cacgtgcaaa tgctatacac aatcctgctg taattaaatc tcctgctcca cttataaatg
    14641 aattaactat ctcatcttgt ttaagccctg atgctattcc tgatattata cctatagctg
    14701 taaatatcat tgcaatttca gttatgtacc attctagtct taaaactcca tatatcataa
    14761 tcgccatacc tattgtgaaa atggctatta ccatggcttc tctttttgta aattccttta
    14821 tatttgtatc actattaacc attaattctt gatttagtga tatatcgtgt actaaagatt
    14881 ttttagggtc ttttttaact ttattggcat acatcataac aaatcctata gatatagcca
    14941 tatatattat aaacataaca actctaaact ctattccaga acctggtact atttgtgata
    15001 atgcttgtgc tattcctaca gagaaaggat tcgtagtagc tgctccaaat ccagctgttg
    15061 ctcctataaa tactgtggcc actgctgtca atgaatcata tcccatactg gtcataagtg
    15121 gaataaatat catataaaaa ggtagtgtct cttcactcat tccaaacgtc gtcccaccta
    15181 gtccaaatag aatcattgaa atcggtatta ttaaaaattg aaacttcttc attttattta
    15241 cagtatgagc tataacagct tctatagctc ctgttttatt aactatccca aaagctcctc
    15301 caactattaa aacaaatcca actacttcag ctgcatctat aaatccgttt ataggtgctg
    15361 taaatacatc agtaattcct tgtggatttg atggtgcttt ttcataagtt ccagctacaa
    15421 caactgacct tccgtctata tctactcttt caaaatttcc acttggcact atccaagtaa
    15481 gaattgccat taacactatt agcaaaaata ttattgtaag tgtctgtggc atagtaaact
    15541 ttttcttttt taaactgctc atttttaatc tccccacttt ttaaattaaa tattttatta
    15601 ttgcatctct ttatcctatt ttgtatagat gtaaaaaagc tcaacatgga tacatgttga
    15661 gctaaatgtg ctaaatacaa catacctgca tcttcccttt atcttgttta agatagctct
    15721 ccatcaaaat tcgggataat tttgatgaca gtactgtacc tattaaatac agtcccagca
    15781 ctagcttcgg acactagcac ttcggcactt actcctttta tattgcctca ttggtttcca
    15841 acctcttcaa tattaatctt agtaaatgca acctctacct caatctgttt gttagatgag
    15901 gtaattctta ttaattttta ttaattcaaa gtaattgtag cataatatgt cgagattgta
    15961 aatattttca gtataataat tcttacttta tgatttcatt ccatttttca caaataattt
    16021 tttctccata taatttaaat actgtagtat attttaaata cacacaaact ttccacaaat
    16081 tttaaattat tataatacct ttataataaa aaataaaagc tatgtaaact tatcaattta
    16141 catagcttta tatacattaa tctactccaa gtacagcttt aagctttgat gctattgtaa
    16201 taactcgtgg accaaatata acttgaactg agtttttctt tttaacaact ccacttgcac
    16261 ccaattcatt tacccagaag ccatcttcat agaatccatc aattgaattt agctctaccc
    16321 taagtctagt tgcacagttt gtaacatttt ttatattatt acttcctcct aaaccttcta
    16381 ttatagaatg tacaagcttt gtatctttat catctacttc ctcttctact gcattgttgt
    16441 atgctgtttt accagcatct acagattgtc tttctctata ttctttctta ctataaagct
    16501 taacttcttc tgaacttctt cctggtgttt ttatatcaaa cttcttgatt gcccatgtaa
    16561 atattatata agtaactata aaaaatattg gtatcaaaaa taataatggt aacgcatcca
    16621 ctttttgtgg ttgtaataaa ttaggaaaca tatttctaaa tgattctccc attatagaaa
    16681 cattgaacat ttctgtaaat acatacgtca atcctgccag tggtacatat gctacaaaat
    16741 acaataatgg tgcaacaaat aggaatgtat actctattgg ttccgttacc cctaccaaca
    16801 tggctgttac tattgctggt atcaatatac ttattgcttt tttcttatta gcattatcag
    16861 ccaacttata catagctaaa gctgctgctg gtaatattgc aaaatgtatt aataaacgac
    16921 ctgttgtgaa gttacgagtt atataagata gtgtatctgg gtctcccatt tgagctaatc
    16981 ttatattatt aacaccctcg taaattgctc cacctacttc cattgttcca cctactcttg
    17041 tatattctat agggaatgca attaagtgat gtataccaaa tggtaataat gctttatcca
    17101 ttgctccaaa agtaaatgtt cctaataatc cactagaacc tatccaatta ccaagtttag
    17161 ataatgccaa ccctactggt ggccatataa ggtacattgc tgtacccaat actattgatg
    17221 ctataaacat cataatcatt gaaaatcttg gacctgagaa aaaagataat gcactaggaa
    17281 gttctatgtc aacaaatttg ttatgtactg cactagctac aaatccagct attatagctc
    17341 caaatatact acaatcatat gaaaagattc ctaagaaaga accatataaa gaacttcgac
    17401 tatatgcttc tgcctctcct aatccagatg ccataaacgc ttctgctgta actgtatctg
    17461 aatttatacc atttgctgct aacaatgtac ttataactgt gtgcattcct ataaataaaa
    17521 caattcctcc taatgcaccc catccttttt cctttttaga taatccaaag gctatacctg
    17581 taacaaagaa tattggtaaa tatctcatta ccataaatcc taaatcgttt attactttaa
    17641 agaatacatt tataaatgac ccttctttta ttagtccgct aagccctaat gcagaaacgt
    17701 tttccatatt tgcaaaagct gaccctaatc ctataaatac tcctgctaca acaagtaata
    17761 taacaggtat catcattgct ccagaaagta cttgtaattt atccttcata gtattatttc
    17821 ctccattttt attgaaatgc caggctatat aatgcatata tatatacttc tattgctttg
    17881 tataatgtat ctatacttat attttcattt gcctgatgtg aatttggatt atccccctct
    17941 attgtgggtc caaatgcaac tccatttttt aacactcttg cataagatgc agctcctttt
    18001 gtaaaaagtt cggcttcttt tccaaaacct tgtttatatg cacttgataa tttatttatt
    18061 aattcactat ctggttttac atacagtaat tttaaatcct tatatatatc aactattaaa
    18121 ttttcttttt ttccaatttc attaattcta ttagttataa actcccaact tataccctta
    18181 ggatatctaa agtctatttt catattcaat tcatttttct ctaagaagat actcattata
    18241 catattgtag aaactcccat atctacatct tctttatata tccctaattt ttttccatga
    18301 ttatcatctg taaagtatga atttaaaata tcccttatta taacgccttt atcatttaat
    18361 tttttaattt tctctaaatc ttttgctaaa ctaaatgcac agttatatga cctgtgtgga
    18421 tttctcgata aagccctttc tcctgtatat cttactaata ctttaccttc ttctagttct
    18481 tctatctctg tataataagc taatgattct actaaatctt tcttatcatt agttttaaaa
    18541 acagcctcaa ttttgtcaca tacaaatcca tcttctttgt ttgactttat atcaactaaa
    18601 ttgtgatttc taaatttatc tttatctatt ttctttctta gattgaagta taaaatacct
    18661 ttttctccat ttactattgg gaagtttcca tctggagaaa atgcaaattt tggctgttca
    18721 ttgacactaa agtaatgctc catacattcc caagtcgtct cttcagctcc acccactatc
    18781 aacctaatct ttctcttcgg cttttcatta agttctctta agaaaagtaa tgcatataaa
    18841 atccctatca aaggtccttt gttgtcatta actcctctac cataaagata gttatctttt
    18901 tgacatactt taaaaggttt actcttccaa agttctttat tatatattgg caccacatct
    18961 atatgattaa gcacccctac tacttcttct ccttctccat attctatatg aattgcatat
    19021 ccatcaaagt cttttacaac aaaatctttg tcttttgcta tttgaataag cttatcgaag
    19081 gcatttctta tttctatccc aaatggttga tttatatctg ttgtactttc atctctaatt
    19141 gaaggtattt caactaaact tctaatatca tttatgatat tttcttgatt ggattttaaa
    19201 taatcaataa ttagttcttt gtttactatc atcttaatta tttatcaccc ctttatatta
    19261 attttacaaa gcatttcagt tcatatctac attataaatt ttgaatatta ttttttcaat
    19321 atttcaaata cttctctaag ttaccataat gatacggtaa acaatcaaca gattatgaca
    19381 aaatcatact atatctatta ctaatatttt aacttatcta ttacgtcttt tttaatgtaa
    19441 gtttattcca aaaatacata aaaaaagatg tagccttagc tacaccttct tatatctatt
    19501 caacttccat tattatgcta ttaatatcta catttccagg ttctatccta tcttttactg
    19561 ttctcttaac agaatcagca tcatccatta taactacagg agttattata tttaaatcat
    19621 ttgacgttat aaaatctaaa tctacttcca ttattaaatc tttagctttt accttatctc
    19681 ctgcctttac cttaacatca aacggctttc cttctagtga cattgtctct aaacctacat
    19741 gtattaatat atttgtatta tcattactct taatcattat tgcatgcttt gtatcaaata
    19801 cattgactat ttcaccatca acaggagaat ataacttacc ttcacttggt attattgcta
    19861 ctccatctcc catcatcttt tgggcaaaaa ctgcatctgg aacatcttct aagttcacta
    19921 aatttccctt aaaagggcta tatattttct caatcttatt ttcttttttc ttaaatatat
    19981 taaacataac acactctcca cattataaaa ttttatgata atatttttct taaagctttt
    20041 gctacaccat catttacatt agtatcagtt acaaaatctg ccatagattt tacttcatct
    20101 gtagcatttc ccatagcaat accaagacct gcaaatttaa tcatggatat atcattttca
    20161 ttatcaccta tacaaataac ttcttcgcta tctataccta atatatcaca aaatttttta
    20221 actgcaatac cttttgaagt acctttgctc ataacttcaa aatttatctt tccagaactt
    20281 actacttcta aatcttcttg ttttttaaat tcttcctttg cttctctcaa agaatctacg
    20341 ttttctgaga aaagtataca ttttaaaatt tcattatcag ctttttctaa taaagtttca
    20401 caacttgcat tttctattat ttcaattctg tcttcttctt tatttttact atttattaat
    20461 ctatatggat gttcttcacc tatttcattg ttggatataa ctattttaca gcctttaaaa
    20521 tgtttgttta aattatactt ttcacctatt gtatagattt tctttagagc ttctttgctc
    20581 aaaaccttct tatactcata tcccagtaat ttaaaatatg tcccattagt tgatataata
    20641 tatatatcat cccctaaaac acttgtaaaa tatttcatag cattataagg tcttcccgta
    20701 actaaggcta ccttgatacc cttttccatg acttctttta gtgctttaat attttcttct
    20761 gatacttcaa atcctttacc catcaaagta ccatccatat cagtacaaat taatttatat
    20821 cgcatttgta tcacatccta gtaagatttt tctaatattt caacaaggtc ttctttcttt
    20881 ccaaaaggta aaaatcctaa aactggacag cttgttattt gttctaattg ttcctttgtt
    20941 acattaaact cagacatttt tgtcttaagt cccactctct ttaaaaaact ttctatttca
    21001 tttcctaaag ttttagctgc ttcatcatct tccacttttt ctaattcttg gtttagcatt
    21061 ctcgctactt tggcaaattt ctctatatta tccttataga acttttctgc aaattttggg
    21121 aacactatag ccaaagcctc accatgagga atatgtgtaa ctcctcctat tatttcactt
    21181 agtggatgtg gtgctgctgc tccagcattt gccagtgaat ttcctcctaa tgtatctgct
    21241 actgctaact ttgttctgta ttctatatta tttttatctt ccattacttt aggtagatag
    21301 ttaatgacta attccataga tttttcagac attatctctg taaaatcatt agcatttgga
    21361 tttatataac tttcaaatgc atgagtaaaa gcatcaaatg ctgttgatgc agtaattctc
    21421 tctggcagag ttaacataag ctctgggtca actatacatt cttttgagaa acaattttga
    21481 tgaaatattg tatttttctc gcttcctctc gatattactg ctgcttgtgt aacttggctt
    21541 cctgttccag aagtagttga tacagctatt aatggtaatt ctttttcact taccttatcg
    21601 tatattttaa atggatttgt atatgtacta aacatataat cccaatctat cttttctaaa
    21661 ccaaaagtca atgctaatac ttttgcagta tctatacttg aacctcctcc aacagctaaa
    21721 acaacatcta cagattcttt tttagctaat tcaaaccctt catttattaa ttctacagtt
    21781 gggtttggct ctactctctg aacctcccat gactaaagtc acagggttcc tgcttcacag
    21841 aattttgcat actaaaatat gtcttactaa ttctccacag gccatccccg tagttcctac
    21901 ggttcttaca taagttattt attttgaatt gttatta
//
LOCUS       DAENCV010000059        21202 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAENCV010000059 DAENCV010000000
VERSION     DAENCV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.060"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(60..749)
                     /locus_tag="KQD82_002248"
     CDS             complement(60..749)
                     /locus_tag="KQD82_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG4380717.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLELMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     EIELSRYFKNN"
     gene            complement(812..1660)
                     /locus_tag="KQD82_002249"
     CDS             complement(812..1660)
                     /locus_tag="KQD82_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4380718.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(1679..2566)
                     /locus_tag="KQD82_002250"
     CDS             complement(1679..2566)
                     /locus_tag="KQD82_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG4380719.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(2624..3427)
                     /locus_tag="KQD82_002251"
     CDS             complement(2624..3427)
                     /locus_tag="KQD82_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380720.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(3587..4039)
                     /locus_tag="KQD82_002252"
     CDS             complement(3587..4039)
                     /locus_tag="KQD82_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG4380721.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(4169..5671)
                     /locus_tag="KQD82_002253"
     CDS             complement(4169..5671)
                     /locus_tag="KQD82_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG4380722.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(5704..6570)
                     /locus_tag="KQD82_002254"
     CDS             complement(5704..6570)
                     /locus_tag="KQD82_002254"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG4380723.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(6655..7326)
                     /locus_tag="KQD82_002255"
     CDS             complement(6655..7326)
                     /locus_tag="KQD82_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG4380724.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(7599..8090)
                     /locus_tag="KQD82_002256"
     CDS             complement(7599..8090)
                     /locus_tag="KQD82_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4380725.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            complement(8112..9476)
                     /locus_tag="KQD82_002257"
     CDS             complement(8112..9476)
                     /locus_tag="KQD82_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4380726.1"
                     /translation="MGEKKINNMNYSHNNYSKNTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NLAD"
     gene            9895..11070
                     /locus_tag="KQD82_002258"
     CDS             9895..11070
                     /locus_tag="KQD82_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4380727.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYNTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEAEAKLKSECLRKII
                     ECCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(11243..12640)
                     /gene="asnS"
                     /locus_tag="KQD82_002259"
     CDS             complement(11243..12640)
                     /gene="asnS"
                     /locus_tag="KQD82_002259"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG4380728.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(12717..12950)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13119..13313)
                     /locus_tag="KQD82_002260"
     CDS             complement(13119..13313)
                     /locus_tag="KQD82_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG4380729.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(13586..15259)
                     /locus_tag="KQD82_002261"
     CDS             complement(13586..15259)
                     /locus_tag="KQD82_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG4380730.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(15269..18667)
                     /locus_tag="KQD82_002262"
     CDS             complement(15269..18667)
                     /locus_tag="KQD82_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG4380731.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSIYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(18835..20031)
                     /locus_tag="KQD82_002263"
     CDS             complement(18835..20031)
                     /locus_tag="KQD82_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG4380732.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKYINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(20052..20873)
                     /locus_tag="KQD82_002264"
     CDS             complement(20052..20873)
                     /locus_tag="KQD82_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG4380733.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         6867 a         3776 c         2233 g         8326 t
ORIGIN      
        1 ttctcttaat aatattttta tttaattaaa attctaataa tactcttact taatcataat
       61 taattatttt taaaatacct tgataattca atttctttaa cataaaaacc tttattgtca
      121 attttaataa gttcttcttt ttgcaatacc tttatcattc tggaaacagt ctccctttta
      181 cagccaagca tatcagctaa atatgtaact gtaatattta catttagtaa agtccattct
      241 ccacagcaaa ctccaaattc tctacttaac ctatataatt ttgctgcaag ttttttatct
      301 acttttatgg atatagaatt tttcaactgt ctatataaac gtcttattct tctttccata
      361 taagaaataa tatcctttgt aagttgaaaa tcattttgca taagttctaa aaattcttca
      421 atactataat aagctacaat tgaattttca aatgcttcac agccaacagt agagttttta
      481 gaagcgtcaa gtgtaatatc atttatgact tccccactat ttaatatgaa aattactttt
      541 ctctctccat tttcacttat cttaaaaata gatacctttc cttctaaaag tatataaaca
      601 ctgtctaagc tatctttttc ataaaataaa atttcttttt tttgtaatct ttttagttta
      661 actttttctt taagttgatt cttcgtttta gtacttaaat tattaaaaat tattacttta
      721 tcaatattat aatcttttaa tttagacatg taatatctct ccattcctaa atttttaaga
      781 gagctctata aaacattaca aatttcttat tttattcatt tttaaacaat tttatagcat
      841 ttgtagtttt aattttcctt tcaatagctt cattagaaag ttcttttact acctgtgtat
      901 atactaaatc aataatgaaa aattgtgcta atttagaaga tattgaaccg gtttccagta
      961 ctgtttctcc cgatacataa gataagtgta catctgaaat atcctttaat tcagaatttt
     1021 tattttcagt aattgatata attttagcat tattggctct tgcaagcttt acagttttta
     1081 taatctcttc tgtttcacca gaatgtgaaa ttgcaattat taaatctcct tcttccatta
     1141 tggaagacat cattatcatt gtatgactac tgtcaaaact tacacaatta agacctattc
     1201 tcataaattt atagtttgaa tcctgtgcta ttataccaga atatccaata cctatgaagt
     1261 aaactttctt tgcatttatt ataagccttg cgcagtcatg tacatcctta ctattgatta
     1321 tctctactgt gttctctaaa gtggttacat ttgaacttaa aagttttttt gctgtatcca
     1381 aagctggctc atcattttgt atatttttat ttataatatt ttttttattg atagttgata
     1441 tttcttgtgc taaagtaact ttaaaatcct gtaatccatt aaaattcatt ttttttacaa
     1501 acctagttat agttgcttca ccaatattgc tctctttagc tatttgtgat attggtttat
     1561 aaaatacatc atctatattt tcttttatat aagctattaa taatttatct gattttgtga
     1621 ctttaaaagt tggtgattcc aattgttcta aaatactcat taaaaaccct tctattcttt
     1681 atattttgtt taaaaaatta tataatgctc caagcattcc tgcatcattt tgtctctttg
     1741 caaattctat attagtgttt ttgtaaactc tctctataag aacctccttt aatgctttgt
     1801 ctatttttgg ttttaggaac ttttcttgtg ccataatccc accacctaaa attaccactt
     1861 ctggatttat tacataacat atatttgcga ttccaactgc taatgatttt atcatattat
     1921 cgagttcttt taaacaatct tcatcattat tctttgctaa atcaaaaatt atttttccat
     1981 ttaagtcatt ttcattcaaa ttttttattt ttgctacttt tttaattaga ctgctagtag
     2041 atgccaattc ttgaaaagaa cttccattta cattcatata tcctacttca ccagcactat
     2101 tactaaaccc atttatcagt ttattattta ttataataca tccaccaatt ccagtaccta
     2161 ttgttaaaca tatagaagat gttgcacctc tacctgctcc aagccacatt tcaccaagtc
     2221 cagcacaatt aacatcattt tcaacttcac attttatatt aaactctttt tctacttctt
     2281 tttttaattg catgccctta tagtttggaa ttaattcttc taatgcaaat agtattttac
     2341 cttcaactgg gtcaaccata cctgcagttg atatacatat accacttatt tcattttcct
     2401 ctatatattt ttttactata tcttttattt tggctaatat tccttctcca cctttttcct
     2461 ttgcttcagt atcaagactt gatttatcaa ttataaatcc atcttctcta ataaaaccat
     2521 attttattga agtaccccca acatctatac aaacgtaatt tttcataaca tatctccata
     2581 caaattattt ttataaatta acaactctat ttaaaatttt atattacaca aatcttttac
     2641 ttatcttttc caattcacag atatatttcc ttgcttctga taagtctata gtatcactta
     2701 atacactttt ttctttggtt atatgatact tataaatatt tttacataag tcctctccta
     2761 acatattaag accttcttga gtgtaatgaa catcatcaat agttaatcct ctgtcatgaa
     2821 actgcatagc aattttgctt cctacacata atatatcaca ttcactacaa gccgactcct
     2881 gtgctgctgc aattcgtctt gttctaataa aatattcatc ttctccaagc caataaccag
     2941 ttaaacttac aaacatttta tcaatcatac attcttcttt aagcctatac gcaatttttt
     3001 ttagactttc tttatagtac aatacatcac taccatattt tgcatcactt tcaccttgaa
     3061 tccaaataac gtatttattt tctatattat aatgtttact taaggtagta cagcctgtat
     3121 taaatttttc tatagtgtca tttagaaacc agttattatc atgattccag ttttttattc
     3181 ttgaaccatc ttttgcattg tgaataaaac aaactttatt tccagtaagc ttattccatt
     3241 catttgaaaa tgcaggtgct atagtaccat caccttctga agtctctagc tcacatctca
     3301 tatttataat ttcatcagta ctcaaatatt caaaacaatt ttcatttgga atgatgctct
     3361 cttttggatt tcccaatcct ctagcattgc tttgtcctat taataagaat acatctatat
     3421 tttccataag ttaactcctt tacaaaattt taagctttta attattttca attttctcat
     3481 aaaaaactaa ggctgaaaat agtaatctaa ctaaaattta cgaagtctca ctaatcgctg
     3541 aaagttaaat cactattttc agccctagtg gttatataaa ttatcattat ctaaccaaaa
     3601 ccttaaaaat agcttttttg atattttctt tttctaaaac ttttattcct gtcatatgag
     3661 catcttccgg ataacaaact aaaaaatcac cttttctaag ttccatgtat ccattatttt
     3721 taccatcaag aggcaagaaa tcgccctttt cttcatatat tttttgttct aaattttcta
     3781 taaaatttat atctattcgt tcacatccat caagcatcaa atgaacatct atatacttct
     3841 tatgagcttc ccagaatcta tcttcttttt cacaagtttt atactctaca atattcacaa
     3901 aaatatcatc accatctatt gtataagtac ctttttcata atctattaat gaattatctt
     3961 tagcatattc aaaacatcta agtaaatctt catgcaatgt tgaatatgtc ttactatgat
     4021 ttatatttcc aaatatcata agcactatct ccctatataa ttataaagtc tatttttata
     4081 cttttatata caaagtatat tatacaatct tatagagtaa ctaaattatt tatctaatca
     4141 ctctataaga caatttatca tttaaatatt atttttcata ataaacagta gtctcagctt
     4201 ttggtaaaga tacttttgct ttaactttag cttctattgc actaactatt gtacctacca
     4261 ctacagaagt cgtgattgtt attagagaat atgcccaaga agttacttct ggcatacggt
     4321 attttaaata aacaactaaa attgctgata ctatgaatcc aacatatgct cccattttag
     4381 tcgctttttt acaaaatgca ccaagtacaa atattcctgc taaaacacct agtactagcc
     4441 ccataaaact attaaaccat tcatatgctg actttatttc tccatttgct aatactattg
     4501 caacagctat agaaagtata cctacaccta atgatatata ttgtgctatc ttagtttgct
     4561 tttccatact tatatctttt gttatgatag tttgtatatc taaagtccaa cttgtagcaa
     4621 ctgaattaag ccctgtcgat aaagttgatt gagaagctgc atatatcgct gcaagtagta
     4681 ttcctgttat acccactggt aattcataag ctatatatga tgcaaatact tggtcttgtt
     4741 gaactgtttg agctaaatct gggttttgag tatagtatat aaataaacaa gttccaatta
     4801 gataaaatac tgttgcaagt cccattgata aaacaccatt tccatatgtc attttattaa
     4861 gttgctttat atcagttgtt gtagtaaacc tttgaactat atcttgactc gatacatatg
     4921 atgaaaaagt attaagacca gctccaataa atattataaa tgcacttgta gatagtatat
     4981 ttttatcaaa cataacttca ttacttgcta agaacttgtg tccagttgta agagtatcca
     5041 ttactgttcc aaaaccacca tttatacttg ctattaaata gaacaatgtc cctgtaacac
     5101 ctactattaa taccatacct tgtataaaat cggtccacaa gacagccttt aatccacctg
     5161 tatatgaata aattattgct attacaccca taactactat caatacattt acaggtattc
     5221 ctgtaagacc agctagaacc actgatggaa gatacattat gatagacata cgtcctattt
     5281 gatatattat aaacatcaaa gctccaagta ctctaagacc tttactttcg tatctcattt
     5341 ctaagtattc atatgctgta tctatttcta acctactata tagaggtaag aaaaatttta
     5401 ttgtcaaagg tattgctata aacataccta attgtgcaaa ccacaaaatc caagtaccac
     5461 cataagaatt tcctgctaat gataaaaatg atattggact taataatgtg gcaaatattg
     5521 atactgatgt aacccaccac ggaatagttc catcaccttt gaagaactct tttcccttca
     5581 tttccttttt tgaaaataac aatcctgcaa ataaaactgc caataagtaa accactagta
     5641 ttgcaaaatc tatccaagta aatcctacca tttgactaat cctcccatac taaaagtatt
     5701 ttattaaaaa tactttctgt atatttcttt tgctcttgat ttcatttcat cagttgcttc
     5761 tttcataggt tgtctacagt atccagcctc aacaccttgt tcttcaagta gtaatttaat
     5821 tgtttgatat aagccatttc ctaatatatc tgttattaaa tcattagtca catgttgtac
     5881 ttcaagtgct tctgatattt tttcattctt agttaattcg aatatttgtc tagctctgac
     5941 accgtttaca ttgaatgtag aaccaattgc cccatctaca cctaaaactg tagctggtaa
     6001 catcatttca tcaaatcctg caaatattaa tttatttggg aatgtttttc tcattctctc
     6061 taataagtag aaatctgctg ctgtaaactt aacacctatt attttttcat tttcaaataa
     6121 ttctccaaac tggtctaaac tcatatcaac accagttaaa aatggtatag agtatattat
     6181 taatctatta tctacagaat ttattattgt attataatag tgttttattt cttcaaaatc
     6241 aaatttatag taaaaaggtg taacagcact tattgcatca tatcctaaat cagttgtaaa
     6301 tttagcaagt tctacagctt cttttaaatt aactgaacca acttgagcta ttaattttat
     6361 ttcttctttc acttcatctt tagctatttc aaatattctt ttcttttcat cagttgaaag
     6421 catgaagttt tcccctgtac ttcctcctac ataaagacca tctactttac aaacatctat
     6481 attgtgtcta attatttgtc ttaacccttt ttcatttata tttccttcct tatcaaatga
     6541 aactaataat gctgaataaa ttcccttcat atctgttgtt ctcatatctt tacctccaat
     6601 tttaattttc aaatatagtt aaatataata attttttaat ttataaatta atttttaatt
     6661 aatttcaatt gctttaacaa atttttctgt tattaattgt ggtcttgtta ttgctgaacc
     6721 tactactgat gaatgaacac ccaactcaaa gacctttttt aaatcttgag gagtatttac
     6781 ctttccttca gctattacag gtatttctaa atctttaact aatctttcca ttaatacaaa
     6841 atctggacct tctaatgttt tagtatatgg agtatatcca gatagtgtag ttgaaacgca
     6901 atctacaccg tactcttgtg ctttgatagc ctcatcataa tttgaaatat ctgccattac
     6961 tagaacaccg ttttctttta tatactttat aagttctttt aagtcttcat tatttggtct
     7021 aactctattg gtagcatcta atgctatcat ttcacaccct gttgttaaaa gttcatcaac
     7081 ttctttttta gttggcgtta tatatatatc tgaatcttca tagtttctct ttataatacc
     7141 tataacagga agtccagtaa ctttttttat ttcaataata tcttcaactc cttgagctct
     7201 aattccaaca gctccacctt ccatagcagc cttagccatt ctccccatta taaaaggact
     7261 atgaagaggt tcatcttcta aagcttggca tgatactatt aatcttccct ttaccttatc
     7321 tagcattttt taaattacct cgtttctgaa atttattttc ttgatttatg tattaataat
     7381 aacatgaaaa ctttttcttt gcaatatttt ttcatatttt tttcttgcat ctatattttt
     7441 actgatttat tttatatatt agcttgtttt ttacatttaa aagaaatttt atttcatttt
     7501 tttggtgttt attttcattt tatataacta ctgttataag tccatttaaa taatttcaat
     7561 aaaaaaaccc atgtatacta tacataggtt tcccaatatt attctaaatc tattataatt
     7621 tttcttccac agcatggaca aaattcagcc tctgtatcta tctcactttc acaataacca
     7681 caaattataa aatcatcaca ttcattttcc aattcaaatt ttttaccaca ttcagcacaa
     7741 tatttatcat ttacatttcc aacataacca cactcacaag ttacattact ttctgccatt
     7801 tccatttcat taatttccat atttttttca taaattaact tatcaagttc tagtaactca
     7861 tcacagaatt tatcaaaact attatcatta atagaatctt ctctaatttt ttgatatgtc
     7921 agtattccca tttccaagaa tatctttgct ttctcttctt gaatatcatc aatctctcta
     7981 tttaattttg atttttcttt atataattca actttaaatt ttccactacc aaactttttc
     8041 ttaaccttag aaaatttatc atctacttta ttattagtat ttccttccac agaaattccc
     8101 tccaatcata tttaatcagc taaatttagc ttatatccta ttaaagatat cacaaaacta
     8161 tatacaaatg aaatcacaat agcttgtaag actttaaatt gcattattaa agttgttcca
     8221 taattagttg tattaaagaa attaatatta ctatctagta taaatgtact aaataaagcc
     8281 aatattccca tcaaaaatgc ataatatata ctaaatacta ttataggtct tatactatct
     8341 gtattatact taaatcttag cttgtatcca tttaataata atattaacat agacaatgct
     8401 cccattgcat aaaacattaa tttagtatct ccaaataaag atgaagaact taaagtaaac
     8461 attgatactg ttgagtttat tatggttact gaaattccat ttgcaaaagc ccacatataa
     8521 ctagctattt gtgggagtat agtaaataaa tttaatccat ttgaataact actcatatct
     8581 agttcattta agtaactact atcagatata gttagtacca atagtattac taatactaaa
     8641 acataaccta atatgaatgt tcttatagaa tttgaaaaaa gagataaata catattttct
     8701 ttttcataac tttttttata agtagttatg tatgtacata taaaccctaa tacgaatcca
     8761 tttaaaagaa cactaaaaaa ttcataacta tattctaatg catatccata ttctaacata
     8821 ctatgtgaac tagtttttac attagtaaaa atagataaaa tagctaaaac taatccataa
     8881 aaaattccaa ctcctagaga atttgctaat acagtttttg aatctctaca tctttttctc
     8941 ataaaaatta ggtttgatat taataatacc aaaataggga ttataagtaa tatcaacaaa
     9001 cctatattgg catttataaa cccagaaccc atcatggtag acatagatac actaatttga
     9061 cccaagttga gagctaggat tatatgtgct ggatttacta aataactaat atcattaaat
     9121 tgaatagtta taatagcttt aaatatcaat gcaataataa acaatataaa tatggttgta
     9181 aatgttgtta aaaatacacc tcttttattt gcatgatatg aaagctcttt tattttagat
     9241 ttcaagtcta gttttgtttc aagtggtctt aatgatgcta tttcataaag ttcatttcca
     9301 caaaatttac agtaattatc cttagtatta ttttctttat tgcaactaga acaaattttt
     9361 tcttctttac ttttaagagt gatatggcta ttttttattt ttctagttgg ttcacctgta
     9421 tttttggaat aattattatg tgaataattc atattattta ttttcttttc acccattttt
     9481 tccctccaag ttttactatt gtaaattatc aaatataact atatcatata aaacttaatt
     9541 ataccttaat tggatatatt tatctatttt atttgtttat tgctgttgtt ttttaacagg
     9601 tcttgatatt taaatagttt gttaatgcga actttattat ttatatttta ttatgcttaa
     9661 tttaaattta ttctattctt tattttcagt agtaaactat ctgtaaatct taattataat
     9721 aaagtattaa tcaataaagt tactcgacat tatctaataa attttatcta tcacaatttt
     9781 agattgttac acacaatata ttttgatata attaaatcat aaacgaagta ttccaaatat
     9841 ctaatgatag aaaggaatac ttatttttat gctttatttt taatggaggc ttatatgaac
     9901 ttagaaaaaa taaaaaaatt ttttggattt cctctagaca aaaaacacaa agacaatgtt
     9961 gagaaagaac tctgcaatta tctttataat acgggtaaaa tcatttgcat aataatagta
    10021 atatctcaaa taattatgat gatatcaatc tcagctagag aaggaggccc ttttgtcaca
    10081 ccaagacgcc aatggtattt ttatatgtat gtaacactat tttgtgtatc ttctgtcttt
    10141 atgtttgtat tttcttacct ttggaataaa aaaaaggatt gctgtacgtt ttatataaat
    10201 ttatggataa tttatgtgac ttttttctgc ctatggggtt gtgtaattac tctacttgac
    10261 cagttaggtg gaaatgacct aagtgtattt agttatatta tgttagcagc tgcagctttc
    10321 tcagtattaa aatttggaga atcagtaata gtttttggag gatgcttttt gtttcttaat
    10381 ttattcttac ctgggattca aactatacca ggcaatgagt ttagtaatat tgtcaacaca
    10441 ttttttataa catcccttgc catttttgtt tctacaatgc tatatagaaa tcgtgtattt
    10501 acttactatg ataaactttt aattagtcaa caatatgaag aaatatctca tataaataat
    10561 ttacttaacg acaaagttat gacagatgac cttactaaaa tgaacaaccg taggtacctt
    10621 gaagatattc tacaaaaaaa attaatcagc catgcaaatc aagaacctat aactggaatg
    10681 atgatagata tagattattt taaacaattt aatgataatt atggacatca aaagggagac
    10741 tattgtctac aaaatattgc caaagtaatt ttagatttta caagttctga aaatgaacat
    10801 gctatacgat atggtggtga ggaatttttt atatgtattt ttaactgtaa tgaggcagag
    10861 gctaaactta aatcagaatg tttacgaaaa attatagagt gctgcaattt tgagcgtgat
    10921 gatgttcctt taaaatgtgt aactgttagt attggagttt atacaaagaa aattgaaaag
    10981 acaataacat taaatgagtt tataagtaat tgtgataagg ctctatatac tgcaaaagaa
    11041 caaggacgta attgtgttgc attttattaa aaagttacat actattacat aaacctataa
    11101 gtattatatc gcaaaataaa agtaatcaaa ttatatattt atagtacaat aaaaataact
    11161 tgcatatctc taaaatagat aaatatgcaa gtcattttag tgtttatact taaagttata
    11221 taggttttat tatatataat tattaaaatt cagcattttt aggagttctt gggaaaggta
    11281 taacgtctct tatgttagac atacctgtca tatacataat tattctttca aatccaagac
    11341 caaatccaga gtgagtagca gtaccaaact ttctaagttc taaataccac caataatctt
    11401 cttctcttaa tcccatttca ttaattctat ctaacaatac atcttctctt tcttctcttt
    11461 gagaacctcc tacgatttct ccaactccag gaactagtaa atccattgct ctaacagttt
    11521 taccatcttc attcattctc atatagaaag cttttatatc ttttggataa tctgtaacaa
    11581 acactggaca attatatatt tgctccgtta tatatctttc atgctcagtt tgaaggtcac
    11641 atccccactc aacaggatat tcaaattcat gaccagattc aattaataat tccactgctt
    11701 tagtatacgt tattctaata aactcagagt ttactatctt atttaatctt tctaacaatc
    11761 ctttatctat aaaactatta aaaaattcca tttcttcagg agcattttct aatacatagc
    11821 ttataacata ttttatcata tcttctgcaa gttccatgtt atcttcaagg tctgcaaaaa
    11881 ctatttctgg ctctatcatc caaaattctg aagcatgtct tcctgtataa gagttttcag
    11941 ctctaaatgt aggtccaaaa gtataaacgt ttctaaaaga aagtgccatt atctcggcat
    12001 ttaattgacc actaactgtt aaattagcct cttttccaaa gaaatcttga gtatagtcta
    12061 ttttgccatc ttcagttttt ggaatatcat ttaaatccat agttgttatt ctaaacatct
    12121 ctcctgcacc ttcacagtca ctacctgtta tgattggaga atgtgcatat acaaagtttc
    12181 tttcttggaa gaatttgtgt attgcatagg cagctattga tcttactcta aatacagctg
    12241 aaaaagtatt acttcttggt cttaaatgtg ctatcgttct taaatattca agcgtatgtc
    12301 tcttcttttg tagtggatac gaactatctg attccccttc tacaactatg ctttttgcat
    12361 gtatttcgac tggttgttta gccccttctg tagatactaa ctctccctct acaagtatag
    12421 atgagcttat tgctaatttt cctacctctt taaaattttc tactttctca tcaaatacta
    12481 tctgcatatt tttaaagaag cttccatcat ttagctctat aaatccaaaa tttttagata
    12541 ctctagatgt tcttatccat ccagctactt ttacagtctt ccctatgtac tgttcttttt
    12601 ctctgtacaa ttcttttact gttataaatt ccttttgcat tttaaatcct cctagtataa
    12661 ttgttattaa tttcttttaa atataatttc aataaaaaaa gcccttcatc cctatataaa
    12721 gggacgaaag actattactc tcgcggtacc accctagttg tcataatgac ctcttagtta
    12781 agcaatgctt aaagcctata acggtgcctt ccgtctaagt ctacttacta aataagcttt
    12841 cgattagaaa ctccaagatg ttcttcaata taaacctcgt attgagcttt caccatcctc
    12901 aactcgctag aactacattt tatatctact cttctcttca cagtatttat cttttattat
    12961 ttctgctaat taacaaaatt ataccaattt ataaattttt agtcaataaa tttatataat
    13021 tgatactgat tgttatttaa aatataataa ttctacctta cataatgata ataaatactt
    13081 atcttcatta cttctttatt acttcaattt ccaaaatttt ataatttact tccattgtca
    13141 acccagtctt ttgcttcttc gacaccctta attgaaggta ttgaaacttt actatgaggt
    13201 tttaattttt caacatcagc ccatgctgct gtcgactcat ttccaactaa aagtgaactt
    13261 ttaagtcttt tattatcttt accttttaat gctcttgcct ttttactttc caaaactatc
    13321 acctctatta tttattttgt aatagtttta gcaattttac aaataaaatt cataagtcta
    13381 aattataatg atttttaaat taacatcata tcttttcaat acttaattta tacctattta
    13441 tatacaaaat atttactttg tgtattcaaa atataaaact attttttaat tttcatattt
    13501 attttttatg ctatttttct actctatcta aagtatttga tacaattaaa ctaataatca
    13561 attataattt tacataggtt cttatctata gagtatttgc tatctgttga atcgtatttt
    13621 ttaaatttaa aatcccatat ccctgcgata catttggata tgtatatatc tcaagttttg
    13681 tagcacccaa tattaaatat gtttttaaaa cttgtgtaaa caatgataat ctcggtacat
    13741 tatcctgttt ttctaagtat tccatcaata aggctaaaac acctgtgact atagaactgc
    13801 tcactcctgt tcctgttcct gtattatatg ttccattttt atatgtggat attatatcta
    13861 cacctgaagc tactatatct ggcttaattc ctcttcctct aattggtcct ttcgaagaac
    13921 caatccacat actatcagtc ttattgttaa atgtaccaac tgtaattaca tcatcactag
    13981 cagcatacat agttatagta gctactgaat cagggtctaa aaaccttgtg tctggagcta
    14041 tcaaattttt atttggcaag tatatgtcat attctccatt tattataagt tctgaagtca
    14101 atcttaaaat ccatactcca ggttttatat ctcttaatct tatctccaaa ttttcttttc
    14161 cagaggtaat atatggataa ataaatctca ttgcataagt agtattttct aagttaaatt
    14221 ttcctctata tatataaaag tctggagaat accttatatc atgactaact tcacctgaag
    14281 gagatattat ctgtgctccc actttatctg gaccattcgt atttagtgtt atatcaagag
    14341 catagtcatc tccatcttgt attattacat cttgaacttc tcccacactg ctaaatctgc
    14401 cagaataatg aatatctgta ttcccttgat ttcctgcacc actcactact accacaccag
    14461 cactactcaa tatattaaaa gtatctagta ttgaagtagt tgctacagca cttgacttaa
    14521 ctccaatagt caaatttatt atcagtggtt tattttctgt tcttgctatg ttagtaacat
    14581 acgttatagc tgctaaaaaa tctgaaacac tgtaatttat ccttccagca taataagtat
    14641 ctgtatatga ttttaattta actactatta agtctgattc tgtagtaata cctctgtact
    14701 gagaatttat ccttccattt ccaactaata taccagaaac taaagttcca gtacctatat
    14761 tatcttgact caaactccca tcatttctat ttatggctat atttaactca cttcttgtaa
    14821 attcacttcc aaaaataaaa ccttctggag gtggatttgt atttgcttct tggtcccata
    14881 aatataaaac tttactagtt ccatcatcat ttataaaatc aggatgcaaa taatctatcc
    14941 cagaatctat aactgctaat aaaattcctc tccctgttat atcattatat ggattttcat
    15001 aaatataatc agtttcagct gctgttgtaa tggtttctcc attatttaca ttattagtta
    15061 tttcaattag actgctcatt ggttcagatt cttcccacca agcaacttgt attatattat
    15121 ttaaaatagt ttcatcaaaa tctaaaggta catatattac tgcaagctga ctatttaaaa
    15181 ccatatacct atttattcca ttttcttgta gggcagattc tatatcacct tgataaatta
    15241 tacaataaga tttttccata aatccctcct atcttaaaac atcaaacatt cctctaacat
    15301 ccaagagccc atatccagaa tttgtatttg gatatactgt gtttgaatct tttctagcac
    15361 cagcttgcat aaaagttttt attttttgca catatgcttg gtttggatat ctaccgtcaa
    15421 caaaagtata ttgaaaatac attgcagcag caccagctgc atgtgcactt gctgctgctg
    15481 taccagttat agttgcatac gtgttcccag gataagcagc tattatatta acacctggtg
    15541 ctactatatc tggttttaac ctgtcttcaa tagttggccc tcttgatgaa ctttgccata
    15601 aactaccatt aatagtattg taggctccta tggttattaa atcatcttga atcgcaggat
    15661 aatttatagt gtaaaaaggg tctacctctc taaatctagt tccacttttt aaaagttctc
    15721 tatttggaag gtataagtta tatcttcctg ttataatata aactcctact aatcgaatct
    15781 tccatacacc tctttttgca ttctttaaag taacattagt aaattgctgt ccagaaaaag
    15841 ttgttgggta tatatatgtt atagaatatt cagtcccctc taaatcaaat aaacctgtta
    15901 ctttattata atttgaaatt ccaacacttt tactttcttc accagtggga gatactatta
    15961 ttacatcagc tttatctggt ctatttaacc aaagttctaa tgataattct tcctcgtctt
    16021 catttaactc taattcaact tcaacagaac ctccaacata tggtattatt ccagatgtat
    16081 gtgtctgtgt attgccctca tttcctgctc ctgccgttat acataaccct ctagtaaaga
    16141 acgctttttc actattactc ctattggtta aacctgctag gctactggta cctaaagaca
    16201 tatttataac taatggtctt ccaagttcaa aagctttttt atatgcatac tgagatgcag
    16261 caaacaacat tgcactatta taaaaatcat ctatcttgcc gagttttatt attattagct
    16321 cagaatcttc tgctatacct gcatattcac tatttacatt tccaagccct gcacatatcc
    16381 cactcaacat agttccttga ccaacttcat cctgagatag actagggtca ttttctgcga
    16441 tagctcgatt tatatcttct cgtgtatatt ctgtcccaat ataaaaacca tcaggaggag
    16501 ttccttcttt tgtttggtcc cataaatata ctatttttga agtgccatct ggataaataa
    16561 aatctgggtg tagatagtct attcctgtat ctgcaattga tattagagtc cctcttcctg
    16621 ttatattaat attaggatta tttttaaaga aatttacgcc catttcttct gttgcaacta
    16681 cacctccaaa agtacctcga ttaatttctc caagtaatgt catctttgta gtcgatacag
    16741 ttcttatgat tgaaggaagt tgcaaaactc gttgtatgct attatagtct gtattattta
    16801 tagatagtac acctaatgaa tcacttatct taaaaaacct ttgactcata cctaacccag
    16861 ctagttcttc ttcaaattca ggagtatgga ctatatctat tcctgatagt attcctgatt
    16921 gtctacttgc atttttactt atataaattt cattctctaa gtcattatgt atttgcatat
    16981 tataaccatc tataaactta taactatttt cctcatcttt aaatgactta ttaatacttc
    17041 tatatccaat atcctctgtt tctaaatctt gatttatatt agatagagtg tacaaatcta
    17101 tatctgataa atttaaaaaa ccaaatcctg atgaattatt tggataagat tgattactta
    17161 atctccttgc tccctttaga agtaatgccc ttaatttctg tgaatacaaa aataaatcat
    17221 ttccatttac aattccccat tccataaaca atgaacaaac ccctgtaaca tgaggtgtag
    17281 ccatgctagt tccagttaat gctccacttg tacctcctgg taaaaaagat actatatctt
    17341 cacctggagc taataaatca ggtttaaaaa cacctaattg tgtatctcct tcaccagaaa
    17401 atatagatac tatatctgtt cttgaattaa aactacctac tgttataact ctacttgctg
    17461 ttccaggaac tgttacagta agctcttgag taggaatcag aaatctagta tttctattta
    17521 gaccttcaga agttggcaaa tatatattta cattaccagt tactatatct attggttcaa
    17581 atacaatttt ccataaccca ggagttatct gtgtatttga acttaattgt agagtaactc
    17641 ttcttgtgag agaatacggt gctatagggt aaaaataacc agttattcta gtttcaccca
    17701 aagtattcct tatctcacct gaagttagag agattgcttg tgtttgatta tttgatgggt
    17761 ttaccaaatg tacactaaaa tcatcaacaa aatctggcca tatatttatg tttaatatac
    17821 gctctccttc cccaactata aactcaactt cttctgtaat attattttga agcctaatcc
    17881 ttttatgacc tcctttatct gcattatttc cagctgctac aactatattg tttttccaaa
    17941 ataaacacat atcatctata tattgttcaa ataaagatgt tcctctatgt gaaccttcat
    18001 tacttccata acttatattt aatgtaacag gcattcttaa ttctaatgcc ctatctaaaa
    18061 taaatttaat tgccctcata aactctgtac ttctcgaaaa aatatcggtc tgtatatttc
    18121 caactctaac cactattatt ctggcatcac ttgcaatagt tgcacaaata cctgcaacat
    18181 gtgtcccatg taagctagtt gttgaaatag gtatatatat acttccatct attgcattat
    18241 ttatatcttc attagtatat aatgttcctt ctctaaaacc ttctggtgga tttccttgaa
    18301 tagattggtc ccaataatat aatatcttag accttccatc actatctcta aacacaggta
    18361 gagtataatc tattcccgaa tcaattatac caattatagt tccttttcca gttaaaccag
    18421 ttctattttt aaatcctgtt attcctgtac ttgaaaaact ttgcacatcc tgagtctgta
    18481 atataaaagg cttttctata aattcaattt ctggataagt taaaagtata tttacatctt
    18541 cttcattact tgatgttatt attgcatatg atgaattaag tatctctact gatacaccta
    18601 attcttcttc taacctcaat atatctccat tgtattttac aattaattca taatttatta
    18661 taatcacctc gacttctaaa attattatta atgtatatgt tttgtgtgag tctaaaatgt
    18721 attagactat ataatttttt aagtttcagt tatacaattt ataatagata tattaaaaaa
    18781 aataaataat gatggtacaa ttttgaataa atcataccat catttatatt tactttatct
    18841 tgaaaccatt aaatttttca atgctttctc aagacctttt acagtcaatg gaaacatatt
    18901 aaattcactt tgtatcattc tttgagtttg ataccaataa ttaattgaat cccatccatc
    18961 tctaagttct ggattcatcc atacaatttt actgtacttt ctcttaaacc ttttaagcca
    19021 ctctatacca ggtgtgtaat tatatggacc acgatagtta ccaccaatat gaagcaactc
    19081 agaaggcgac atagaagcat ctccaacaat tataacttta tagtttttat ctatattttt
    19141 taatatccat tcagtattaa tatatttact taaccaacat tcaggtgttg taaataatct
    19201 atcataaata cagttatgga aataatatat ctttacatct ttaaaatgat ttgatttact
    19261 tacagactga aataatgtat tacataaagt gctataccct ctcattgaac cacctgaatc
    19321 cataagcaat aaaagcttca ctgtattttt tcttggcttc tcaaaaaata actttaaata
    19381 tcctgcatta ttacaagtct cttcaatagt cttatccaaa tcaagttctg tctttggagc
    19441 atctattcta gtagaaaatt gacgaagtct ctttagtgca acttgaaatt gacgcatatt
    19501 taaaacttca tcatctctaa aatctctgta ttttctttct cctgcaactt caagaacagc
    19561 tctaccataa gtagaatttc cacctatacg tataccagtt tgcccttttc ctgaatgtcc
    19621 taactctgaa gtgcctcctg tgcctatcca ataattacca ccattatgag gagagtcttg
    19681 ttcttttatt cgttcctcca atttagctct taatttatca agatttatat ttggattttg
    19741 ttctaatctt tcaaactctt ctaaatctat atctggtttt ttaagccaat tcataatctc
    19801 ttctggaatt tcttcttggt gttctatacc tttaaaatac tccaaaaaca ctgcatcaaa
    19861 cttatcaaaa tcactttcac tttttattaa aatcatcctg caaagatagt aaaaatttga
    19921 aaagtttgaa tagcacagtc ctttatccaa tgcttcaatt aaagtcatcc attcatttaa
    19981 agatacattt aatcctcttg acctcaataa ataaaaaaat tcaataaaca taattatcca
    20041 gctcctatta actataagtg tctttgcata gattcaatat cctcattatt cttaagtagt
    20101 attcctgcaa aaggaacttc tttttctatt ttttcaatag gcattccact caaagttaat
    20161 gcttgtatcc agtctattaa ctctgatgta ctaggctttt tttgtatgtc ctttaaactt
    20221 ctaatccaat aaaaagtagt cattacttgt tccaataggt gttcttctac tttatcaaaa
    20281 tgtactttaa ctatttcttc catcatgtct ctatcaggaa attctatata atgaaatatg
    20341 catcttctta aaaaagcatc tggaagttct ttttccgcat ttgaagttat aataactatt
    20401 ggtctttgtt tagctcttac agtttctttt gtctcattta tgtaaaactc cattttatcc
    20461 aattcccaaa gcaagtcatt tggaaattct aaatccgcct tatctatttc atctattaaa
    20521 agaattactt gttgatttga gctaaaggcc tctcctagtt taccatattt tatgtacttg
    20581 ctaatatcat ctacaccttc tcctccaaat tggctatcat aaagtctttg aacagtatca
    20641 tatacataaa gcccttcttg tgcttttgta gttgatttta tattccaaat tactaaatct
    20701 ttttttaatt catttgaaat tgcttgtgct agcatagttt tacctgttcc aggttcgccc
    20761 ttaatcaaaa gtggcttttc aagagcaata gcaacattaa ctgatgccat caattctggt
    20821 gatactacat aatcactact tcctctaaag ttagatatct ctttttttat cataaatcct
    20881 actcctttta aacagtattt tctaaatgta tatagacttt attactattt ttgatataaa
    20941 aataataata caatacattt gtatagtatt attctaaaat tctatatcta aataaaatca
    21001 cctctaaaat atttaatttt tatctttatt tgtcataatt aaatgaaata tatattttaa
    21061 agaataataa aagatagctt attgtacaaa atttaaatta taaaaactta gtttaatcaa
    21121 aggcaccact ttttttcatt ttagattata tataaacaca atataaataa tttttattat
    21181 tttagctata aacataaagt tt
//
LOCUS       DAENCV010000060        21066 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAENCV010000060 DAENCV010000000
VERSION     DAENCV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.061"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            392..1918
                     /locus_tag="KQD82_002265"
     CDS             392..1918
                     /locus_tag="KQD82_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG4380734.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VKQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1946..2503
                     /locus_tag="KQD82_002266"
     CDS             1946..2503
                     /locus_tag="KQD82_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380735.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            3025..4068
                     /locus_tag="KQD82_002267"
     CDS             3025..4068
                     /locus_tag="KQD82_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380736.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VEGEKIDLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4179..5321
                     /locus_tag="KQD82_002268"
     CDS             4179..5321
                     /locus_tag="KQD82_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG4380737.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYIFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            5775..7166
                     /locus_tag="KQD82_002269"
     CDS             5775..7166
                     /locus_tag="KQD82_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG4380738.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            7419..9212
                     /locus_tag="KQD82_002270"
     CDS             7419..9212
                     /locus_tag="KQD82_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4380739.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(9293..10675)
                     /locus_tag="KQD82_002271"
     CDS             complement(9293..10675)
                     /locus_tag="KQD82_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG4380740.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            10933..11280
                     /locus_tag="KQD82_002272"
     CDS             10933..11280
                     /locus_tag="KQD82_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG4380741.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            11298..12332
                     /locus_tag="KQD82_002273"
     CDS             11298..12332
                     /locus_tag="KQD82_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380742.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(12381..13430)
                     /locus_tag="KQD82_002274"
     CDS             complement(12381..13430)
                     /locus_tag="KQD82_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380743.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            13705..15240
                     /locus_tag="KQD82_002275"
     CDS             13705..15240
                     /locus_tag="KQD82_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380744.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(15425..16111)
                     /locus_tag="KQD82_002276"
     CDS             complement(15425..16111)
                     /locus_tag="KQD82_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG4380745.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            16236..17618
                     /locus_tag="KQD82_002277"
     CDS             16236..17618
                     /locus_tag="KQD82_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4380746.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIQEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            18020..19288
                     /gene="eam"
                     /locus_tag="KQD82_002278"
     CDS             18020..19288
                     /gene="eam"
                     /locus_tag="KQD82_002278"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG4380747.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(19381..19737)
                     /locus_tag="KQD82_002279"
     CDS             complement(19381..19737)
                     /locus_tag="KQD82_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380748.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(19888..20982)
                     /locus_tag="KQD82_002280"
     CDS             complement(19888..20982)
                     /locus_tag="KQD82_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG4380749.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         8375 a         2374 c         3451 g         6866 t
ORIGIN      
        1 gaatataagt aagaattaca attaatttat ataaaataaa tttttaagta aatattttta
       61 tagagagatt gctatattac atatcagaaa aatattaaac taactaaatc aataaaaaag
      121 ttggtggcta acagtttata attgttagca caacatcatc ttatgtattg aaatctaaaa
      181 tatcctcatt tttacttagc ataaatacta ttaattaaga aaataatata gaaattataa
      241 atatatataa gtttagtctg gtgtataaaa caagaaaact aagtgatttt taatttagct
      301 ttttattaag cttatttttt atactattta taataaataa actccatgat gggaaaacta
      361 ttactattta atatgtaaag gagtatttta aatgaatgaa tcatattggt ttttaaactc
      421 aagtccaaaa gaatataaca aattaggtga aaatataaaa acagattgtc ttatagtagg
      481 tggtggaata actggattga caactgcata tttattagca aaagaaggta aaaaagtagt
      541 gcttgttgag gctgataaaa taggatatgg aacttctgga aggaatactg gtaaggtaac
      601 ttgtcagcat gatatatttt actctaagat agagaaaaaa tatggattag ataaagcaaa
      661 atcatattat aatgcaaata atgaagcttt aaatttagtt aaacaaataa tagaagaaaa
      721 caatataaaa tgtgatttta aaagggaaac ttcttttgta tttacagaaa aagaggatac
      781 tataaagaat attaaagatg aatatcgtac atgtaaaaaa ataggaataa attgtgagta
      841 tcatgaaact atagaaaata ttccattaga tataaagggg gctattagtt ttactaatca
      901 aggtcaattt aacccaaaga agtatattga tggattggca aaagcagcag taaatttagg
      961 gctaaaaata tatgaaaata caccagtagt agaccttgaa aaaggaaaaa tatgtagggt
     1021 taaaacaaga gaagataata taatagaagc tgagaatgtt ataatttcat ctcatagtcc
     1081 atggtatgat ggattgaatt tttattttgc aaaagaatat gcagagcgtg cttatttaat
     1141 ggttgcagtt ttggaaaata aattagcaga tggtatgttt ataagtatag atgacccatc
     1201 aataactttt agacaatata atgatggaag tgaaaattta ctgatttttg gaggaggaga
     1261 ccataaagtt ggtcagggag gaactgagaa agaaatattt gatgatttag agcattatgg
     1321 taaagaagtc tttaaggtaa aagattttaa ggggaaatgg tctgctcaag ataatatgag
     1381 ttttgacaat gttccttata ttggttatat caacaaaaga gaagacaata tttatgtagc
     1441 tacagggttc tctaaatggg gaattactaa tggaactgct gctggaataa taataaaaga
     1501 tttaataata aataataatt ctgattataa agatactttt aatccatcca gactaggtag
     1561 ctacttttca aaagatttta taaaggaaaa tgcaaacgta gcaataaatt atgtaagtgg
     1621 gaaattaaaa ataggtagtg gagatatgcc taaaaataat ggagaaggaa agattgttaa
     1681 tattgatgga aaaagatatg gtgtatataa ggatgataat ggagattttt atattgtaga
     1741 cactacatgt actcatttag gatgtgaact taattttaac agtgaagaaa aaacatggga
     1801 ctgtccatgt catggttcta gatttgacta taagggaaat atacttgaag gaccagctct
     1861 taagccatta aaactttatg gacatggtga taatgatgtt aatcctaagt tattataaaa
     1921 ttatttttag gaaggtaaag tgtatatgaa taaactttta aagccaataa ctatactatt
     1981 gtatatagtt tatattttat ctgccgtatt ttttgtagca aacaaagaat atggaagtct
     2041 aggtctagta gcaattagtt tagttggtac tttcattttg agttttataa ataagcaaac
     2101 tgaaagatta ttgagtaaag aattatatat aatgcttgct ttatttataa tgttttcatc
     2161 attgttaggt acttgtttta atttttatga tataaattat tatgatgatt ttttgcattt
     2221 atggtcaggt ataatatctt gtattgtggc tttttctata attaaatact tttatacaca
     2281 aagagagata agtaaaatga gtaaagtgtt tttagttata tttttattta tgtttagtat
     2341 gggagtagcg agtttatggg aaataggtga atttttaatg gatacttttg ttgggactac
     2401 tacacaggcc ggagggttag aagatactat gattgatatg gtagatgctt taataggtac
     2461 aattataact attcctttta tagtaaaaaa atctaatata tagttttaaa ttttttatat
     2521 taattataat ggatttaatt aatcatgttg agtttcaagg tctatatgat taattaaatc
     2581 catttttgtt atttataatt attattgaat atatttttac atataagata taaggtgata
     2641 gatagaaatt gttggattat gtaaataatt aatatataat accattatgg gcaataaata
     2701 caaatcaaat gataatatag tctatttatg taaatgttaa attgtatcct aagtataata
     2761 gtaagttttt tgatagtggt gtagaaagta aaaatctact agcctaaatt gatgtatgga
     2821 ttagtttagg aacagtaatt gcatatctta agtttgatgt catgttgggc tactagaaat
     2881 aggtcatctt tttcacttga atatcttttc tttaaacatg gggagtatca tatcaaaaac
     2941 atatgaaaat taagtgattt atgagaacag taatatttta tatgatagta tagaatagta
     3001 ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt ttggtattgt gaaaaaaaga
     3061 caaaaaagtg agtcagtaaa agaagaaggt gaagtaatat taaaaaacga aaaaatatta
     3121 tctgaagaaa agttgataga tgaagaagga gttgtagtta atattgataa tgaaatatta
     3181 actaaagtag aagtagtaaa tgatgacaat gaaatagaag aaaaaataat agaagaagat
     3241 tggttaatta gtgaaaatac cataaaatta gatgataaag aagcaattat taatgataaa
     3301 aacatagaat tatgtaaaaa agaagttcaa gttgaaggtg aaaaaataga tttaaataag
     3361 tttgaaggac ttgaccaaaa tgaaaattat aatctagaaa aaaatgttat tgaagaaaaa
     3421 gaagtaagtg aatgtttgac agaagaagat ttagagtaca taaaagaaat taaaataaaa
     3481 agaggaaaaa gtataaaagc tataaacttg tatactaaag aagagtgggt ttttgacact
     3541 catatacagt gtagtaaaaa actcaaagtt ccattagggt acataagaga aaatttaaaa
     3601 tatggatata tggattactt tggagatgca ataaattatt taagtgaagt attaaatata
     3661 gatgaatact gcaaaagtga atggagctat ctcgataata gcaaatctcc atctgagata
     3721 tttaatattc taaacaataa aatatttagt ataaggcttt ctaatgaaaa aagaaatgaa
     3781 attttgacaa atgataaaat tgaagcatta aaaatgaatt atagatttga atgtattgat
     3841 gaagaatatg atgaatattt taaaaaatat aagtctataa tcaaaagagg tggaaagaaa
     3901 aaagttgaat tagtaaataa aaaaggtgac attttagaaa tatttaagtc tttagaagaa
     3961 tgtgccattt atttgcaaaa ggaaaagaat gaagttatac aaatgttaaa atatggagat
     4021 acaaaagtag gaagaaactt tataaggtat agtttgagaa gtatttaatt tcattttaaa
     4081 ttcataagag tttcatttct gattcacaca tttaatatta ttttaatgta aaataataaa
     4141 ggtgaagtat tgtacaatta aaattaagga gggtattaat gtctgaagaa aaaaatccaa
     4201 aagaaaatac attagatgaa tctaaaaatt cagataatgt aaattctaaa aaagatgatg
     4261 tgtcagatga aaaaaatact gataacaaag ttaaaaaaag ggaaataaag ctaaacaaca
     4321 aaacacttaa agctattgta gcaattgttg cagtaggagc tatatgttta ggtggaggct
     4381 atatttttgg caaagaagct ggaagaaaat tgcctgctac ttcaaaacac tataatagca
     4441 gtgaggtagt agctactatt ggggataaaa aaataacagg tgaaatgtta agaaagagaa
     4501 tggagccatt attttattta aatgcaaaaa ctactatgac tgatgacgaa atacgttcat
     4561 atgaatcaaa tatgattgaa actatgatta catctgatat attatcctct gaagcagcaa
     4621 aagagaaaat aagtataact aatgaccaac tagatgctca gtataataat ttaatgagta
     4681 gtatagaatc agcaatggga atgactaaag aagagttttt aaaacagttt gatttgactg
     4741 aaaaatatat aaaagatgat atgaaaaaag agctagtagc atcaaaatat ttaaatgaaa
     4801 aatcaaaagt tactgataaa gaagcagaga actattacaa taaaaacaag agcaattatt
     4861 tacaagttag agcttcacat attttaataa aaactgttga tgataaaggt aagcaagtta
     4921 gtagtagcaa aaaagctgaa cttaagaaag aagctgaaga aatacttaaa aaagcccaag
     4981 ctggggaaga ttttgctact ctagctaaaa aatactctga agatagtagt gcagaatcag
     5041 gtggagattt aggtttcttt ggtaaaggtc agatggtgga atcttttgaa aaagcagcct
     5101 ttgcattgaa gaagggagag gtatctaaca aacttgtaga aagtgattat ggatatcata
     5161 taattaagaa aactgatgaa aaatatcagc catttgaaga ggtaaaatca gatttagtaa
     5221 gcagtttaac atcagaaaaa caaaacctac ttatacaaaa tctaaaagaa aaatataatg
     5281 ttaaaattac agataaatat caaatgaaag ctaaagaata gataaagtca atttattaaa
     5341 aaaaagaaaa attaactgta tggataaaag ctatttttac tctcaatata gagagtataa
     5401 aatagctttt attattttgc aataaaatct atttatatat atgaagtgac aaagaaataa
     5461 catgcaaaaa gacattaaaa agaaatattt ttaatttgaa ttttttcaat aaaaatttac
     5521 aaaaatgaaa aaataaacaa aaaagaattg aagatttaag ttgttattta aatatatata
     5581 tattaagatt ttcttctgaa gatactaaaa aaataatatt tttcataatt ttaaaaataa
     5641 taataaaaaa atgaaaatac aggcgataat ctattgagta aacatagaaa agatgatatt
     5701 ataagtatgt gctaaaatgg ttaaagcaat attaatatta aatttaacta taaaacaaaa
     5761 gggggcgttt ttttatgtca agtacagaaa gcgtatctaa aaaaagtggt catccgaaga
     5821 gtttttggct tatttgcatg acaattttgt gggaaagatt tagttattat ggtttaacag
     5881 ctttattggt attatttttt acagcaagtg tagctcaagg tggtttaggt ctttctactc
     5941 aagaagcaac atccatgttt ggcttattta caggtcttat atatcttaca ccattagtcg
     6001 gtggctggtt agcagataaa gttttaggtc aacaaaaatg tatttctata ggtgcacttt
     6061 taatagcaat aggagatttc gtactatttg cttcatctac atctattgcc atggtttggg
     6121 taggtctatc aataattatt ttaggaaatg gtttttttaa atcaagttgc tcaaatatag
     6181 ttggggatat atatcctaaa actgatgtgt caagaaaaga tgcagcatac agtttattct
     6241 atatggcagt aaatgtagga gcatttatag ctccaataat atgtggattg ctttctgata
     6301 atgtatttgc agtaagagga gcaagtggag atatagtatc ttatggatat aagatggcat
     6361 ttttaatttg tggtataggc atgatgttag gtatgattat atttacactt cttgcaccaa
     6421 aattattaaa tgaagtcgga aaatatcctg tgactaaagg taataaaggt gaaaaaattg
     6481 agtatggacc acttactaag ttggagaaaa ataggataat agcaatggtt attttatttt
     6541 tatttgtggt cttattctgg acagcatact atcaaacaca aagttctttt atgatttatg
     6601 taagggataa tgtaaataga actctatttg gatttgaaat gccagtagca tggcttacat
     6661 cactaaatgc tatattatgt gtggctttat gcccagtatt agctacccta tgggttaaat
     6721 tatctcatac caaaagaggt gatttctcta ttccagttaa aatgggatta ggaatgataa
     6781 taatgggtgt tgggtttata gttatggtat ttgctacatt agctaatggt ccagatggaa
     6841 tggtaaaggc tagtatattg tttatagtat tgacttatat atttacaaca gtaggtgaat
     6901 tattcttatc tcctatagga cttgcaatgt tcaataaatt agctccagct aagtatgcta
     6961 cgttgtctat gggagcctgg tatctaacaa gtttttttgc aagtttatta tcaggaaaga
     7021 ttgctggttt tacagccaca cttggttttt tacagatatt tggaggaata gcaacagttt
     7081 taataatatt tggtttaata ttattgggaa taagaaatgt attaacaaga ttaatggcta
     7141 tggatttact aacaaaagaa aaataattca tgacgaatta ttgtaaataa atgaattact
     7201 ttaaataaag gaagttacat ttaaatataa aattaaatgt aatttcctct ttgattatga
     7261 aaaaaagctt tacacaaata gatattataa ttaaaattat aattgtgata attagagtgt
     7321 ataagattta tataaattat acaaaaaata tttatgtaat aaactttaca tacttatata
     7381 tttagttatt caaactatta tttcgaggag gtcttgttgt gaatataaaa gatagattaa
     7441 gtggattaag aaaatttatg gaagaaaaaa atatagatgc ctatatgatt ccatcttctg
     7501 ataatcacca aagtgaatat gtaggagatt acttcaaatc aagagaattt atatcagggt
     7561 ttaatggttc agcagggacg gttattgtga ctaaagacga agctgggtta tggacagatg
     7621 gaagatattt tatacaggca gaatctcaat tagaaggaag tacaataaaa ttattcaaaa
     7681 tgggacaaga aggttgccca actacagatg aatatttata taaaaatatt cctgaaggag
     7741 gaactttagg atttgatggt agagttatat ctgctagaga aggagctaca ttagctgaaa
     7801 aattatctaa aaaaggtata aagatagaat atcagtatga tttaatagat agtatatggc
     7861 cagatagacc tgctttatca gatagtaaag catttttatt agatgttaaa tattgtggag
     7921 aaagttttag ctcaaaatta gctagattaa gagaaaaaat gagtgaaaaa ggtacttcta
     7981 ctcatgtaat aactactctt gatgatatag catggttatt caatataaga ggtggagatg
     8041 ttaaatataa tccagttgta ctttcttatg cagtaataac tttaaaagaa gtttatctat
     8101 ttgttgatga aagtaagtta aatgaagaaa tactaaatga actagctaag gaaaatgttc
     8161 aaataaagcc atataatgat gtgtatgaat ttgtaaaaaa tatagacaaa actgaaaaag
     8221 ttttattaga tggaactaag cttagttata caatttacaa taatatacct tgtgaagttg
     8281 agaaagttga tgagttcaat ccagtaatgt tttttaaagc tcaaaaaaat gaggtagaat
     8341 tagaaaatat aagaaattca catgtaaaag atggtgtagc tttcactaaa tttatgtact
     8401 ggttaaagaa aaatgtaggt aagatggaaa tcactgaaat aagtgctact caaaaattag
     8461 aggacttaag aagagagcaa gaaggtttct ttgagccaag tttcaataca attgctgcat
     8521 ataaagagca tgctgcaatg atgcactata gtgcaactcc agagtcaaat tataaattag
     8581 aggcagaagg attattttta gtagattcag gtggacaata ttatgatgga actactgata
     8641 taactaggac tacagtttta ggacctataa gtgatgaatt aaaactacac tttacttctg
     8701 tagcaagagg aatgataaat ctttctaagg ctaagttctt gcatggttgt agaggatata
     8761 acttagacat actatctaga agttgcatgt ggaatatggg aattgattat caatgtggaa
     8821 ctggacatgg tataggattt gtgctaaatg ttcacgaagc tccaaatgga tttagatgga
     8881 gagtagtacc tgaaagattt gatagtgcag ttcttgaaga aggaatggta actactaatg
     8941 aaccaggtat atatatagaa ggttctcatg gtataagaac agagaatgaa attgtagtta
     9001 gaaaagctga aaagaatttc tatggtcaat ttatggaatt tgaagttgtt acgttagctc
     9061 ctattgactt agatggtata gtaccagaat taatgaataa ggatgaaaaa gattatttaa
     9121 actggtatca taagttagtt tatgataaaa tatcaccttt cttaactgat gaagaaagag
     9181 aatggttaaa agtttacact agagctatat agtttgtact agatacaata aaaatcgagg
     9241 tattataatg cctcgatttt ttattgtata ttttattaac tttaaaaaat ttttaatttg
     9301 ataattctac taatttttta ttcattataa gtagtataat tccaaatatc aataaaaatg
     9361 atggtatcac tatgaagact tgtaacatac caagttggtc tataatagct tgcgtatagc
     9421 cagataattt atttgctgca aatttgccta aaaaccaaac acccattagt aatgaaatat
     9481 acttttcagg cgaaagttta tttaccatac catacccgac tggagaaaag caaatttctc
     9541 ctatactttg gaatgttaaa aatccaacta cccaaaatac acttgctttg tgaattgaat
     9601 tagggtctgt tcctgtttgt ataactgcta ctatcataaa caaaaatgct atggctaata
     9661 gtataaaacc taagctcatt ttttttggca cacttaaatc gcctttttta gtttgagata
     9721 attttaccca tactaaagca gagataggtc caagaataac acaaaggaga ccattgtatg
     9781 aatctatcca tgaagtagga atttgaaaat tgccaacagt aagatttatg tgttcctttg
     9841 tatagagtgc aatagacata gaggcttggt tatatgctat ccaaaaaaat actgtgaaaa
     9901 aagctaacac caatattact attattcttt ttttctcaat caaagttaaa ggtcttgaag
     9961 cgagcatttt cttatattct agtgattttc ttatagactc tctcccgtta atagggtcat
    10021 tattcaaagg tttataaaac tttctgtttc caaatataaa gaagaataaa cctattagaa
    10081 ccgtaattga tgaaaataaa aatacatatc tatatccata catacttata gataatccgc
    10141 ttttcatagc aaaccattca ttagcaacta aaccgcatat taaagggcca aataaagtac
    10201 ctagattagt agccatatac gtaatagaga aagctccatc ttttcttggg tcatcattgg
    10261 tatataagtt tccaaccata gtatgtaagt ttcctttata aaaacctatt cctaatccag
    10321 ataaaaatat catgatgttt atatccatct tactatttgc tatgtatcct agaaaataac
    10381 caattgaaac cattagcatt ccaagtataa tacagttttt gggacctaaa aatctatcac
    10441 tgacccatcc accaataact ggagagaaat aagtccatgc taaaaaagat gcaagcattg
    10501 atgcggattc tgctggtgtt aaccctaaac cacctttgga gacttgttca gctaaaaaca
    10561 ataataatac caatttcata ccatagtatg caagtcctac aaaggtaaat attgaacagc
    10621 agaaatataa atctctaggt tgtttaagtt tttcttgcgt ttttaaaact gacatatgtc
    10681 gagaacctcc agataaatag tttaacaaaa ttttaccata ctatatgagt atatgttttt
    10741 ttcatataga cgtcaacttg taagataatt taattaagtc gaaatatatg taaaaatagt
    10801 atcatttttg tgtattcagt aatatttttt tatgataata tatgttatta ttatattaaa
    10861 atacaaacat atttatggaa aggatatgcc aatttagtag ttagattgat tgtttggcag
    10921 gtgggatgag ctatgataag aaagaaatct gaattacaag tagaagtaaa agaaaattta
    10981 aatggtggta taggaaaggt taagcttgaa aacataatac aaaatgaaga gcttaaagga
    11041 aaaggaagac tatttaaaag agtagcttta ccagtaggaa gttcaattgg tgtgcatgac
    11101 catacaacag attttgaagt atattatata ctaaagggaa aaggtaaagt atttgataat
    11161 ggggaatttg tagaagtaaa tgagggtgat gtagtgtata cagctgatgg cgaaaaacat
    11221 tctatagaaa atataggaga agaagatctt gagtttgtag cactagtttt atatatgtaa
    11281 aatatggagg aggagtcatg acaggcaaga tagaagaaat taaggacaag ataaatagat
    11341 tttgcaactc caatttaaat caagaataca aagatataag ctttaaaatt ttgcaaaacc
    11401 ttttagataa taataaagag ataattcatt cttctagagc tgatatatgg tcttcagcta
    11461 tactaaatat agttttagaa caaaatcttt tatacaataa gaagcatcca ctacatataa
    11521 caaaaaaaga gttttcaaaa caaataggtg tttctttaaa tactataaat aataaatcgt
    11581 ctttaataaa agatgtttgt agtattgact ttttaaatca agatactata gctatttcta
    11641 atgtagaatt ttgggtaaaa gagactaatt ccaaatcaaa gtctgtagat ttagatttag
    11701 aaaagaaaaa aatactatat aaaagatata ttaagctatc acaagatgcg aatagtgata
    11761 ttgaatcaat tagatatatg gaggaagctg taaatatagg taaaagtatg attactaaag
    11821 aagataaaga acttggattt tggaaaggtt tatctaccag agcctatatg atagcacttg
    11881 aatcccttgc tagaaagtta gaaagtatta ataatctaaa ggagtccaaa aaagtattag
    11941 aatatttaat agaaattaat cctagtgatg agcagggaat aagatacaaa ttattaaatg
    12001 tattaattag attaaatgat agaacttcaa taaataaatt atttgaactt tataaagaag
    12061 aaaaatctgc tacatggatg tacagtaaag ctctttatta ctttaaaaat aaaagtatgt
    12121 ttttggcaaa tgattctatt aaggttgcta gaagtaaaaa taagtacgtt ggattatatt
    12181 tgttagattg gcgaaatgct tttggtagag aatttgttac agaggaagaa aaggcagaag
    12241 ctgtctatta ctatgatgaa aacatagtga tttggaatga agttaaaggt tctatggatt
    12301 ggctattaaa aaaaatgttg gaattttcat aaaaaaaact actccttact tttatataag
    12361 taaaaggagt agtttttaat ttatacatct agttttaaat cttcaggttt tattagacct
    12421 ttttttctca taaattctga aatcaacaaa gttacaatag caggaagtat aaaattaagt
    12481 aaaagtatac caacccacat agatatacct ccttttccag cactttgcat agcagttact
    12541 gttccaaatt gtccaaccaa tccacaagtg cccataccag aaccaatagg aatattctcc
    12601 attttgaata ttgttgtaga taatggtcca agtaatattg aagctacagt aggtggtatc
    12661 caaattttcc aattttttat tatattaggt acttgcaaca tagatgtccc aagaccttgt
    12721 gccacaaggc caccaactcc attttcccta aagctcataa cagcaaagcc taccatttgg
    12781 caacaacaac ctacagttgc tgccccacca gcaagaccac ctaaacttaa catcatacaa
    12841 atagctgcac tacttatagg taatgtaaga gctattccaa caagtgcaga gactaaagct
    12901 cccattatta taggttgcat agttgtagct tgcattatta tatttccaaa tgaagtcata
    12961 aaagcagaaa cagctggacc aataagttca gctacaagta ctccaattag tattgttaca
    13021 gcaggtgtaa ccaatatatc tatttttgtt tcttttgaaa ctaattttcc aacctctaca
    13081 gcaaaaagtg tagcgacaaa tgctccaaca ggtcctccta attcattacc acaagctcca
    13141 acaagtgcac aagaaaatag tacaagtgga ggtgcttgaa gtccatacgc aatagccact
    13201 gctatagctg gaccagtaac ctgtttagca agagggctta taacttcagt aaaaagagaa
    13261 atattgaaat tatctcctaa tgtattgaaa attgttccaa ttaaaaggga cgaaaaaaga
    13321 ccgagtgcca tataacttag tgcgtcaata agataccttt gaacagaaat ttcaatattt
    13381 tttcttttag caaatgattt gaaatttctt tttttataat tgatttccaa tttaaaatcc
    13441 tcctcatata atataagtat aatttctttt taattaatta tataactttt cagctgacaa
    13501 gacaaatgtt atttcactaa aattagacta ttgtaatagt aagaaattaa cattaatttt
    13561 agttttaaat tttaaattta tggtttataa ttagtatagg gatattctga aatataataa
    13621 ttttccagtt tatcccaata tttaacttag gtagattgct tacaaaacaa atgattatat
    13681 attaggctaa aaggaagtgg atagatgata tttgtaaaaa ataagaaata cagaaaaatt
    13741 gccataatta tatctattcc tatattgctt gttgcgcagt actacctttt taaatttgga
    13801 atacttagac caatggtaaa gggagtagaa attgaaattg tagatgggga atatgttaaa
    13861 gatatagata aatatgtagt aaaattaaat gaaacagtta ctttgtctac aggtgaatat
    13921 attaaggttc cttcgtatgc taaaaatcca aatatttggt ttaatgtatt ggataactca
    13981 gggactttaa aaatagatgg aaataaaatg acagcattaa aggttgggta ttcttctgtg
    14041 ggtatcatga aaaattcaag agtattaaaa aaggctacta taaaagtagt agacccagag
    14101 gtagaatcac tagatatgga gattaatggg gatttaaaac atgttggtga tagtgctact
    14161 atagaaagta caatagaagt tgattatgat aagtttaaaa aatcatataa gccagtttat
    14221 aagagtagca atgaaaatat tttaaaaatc aaaggaaata aagtaagtgc agttggagtt
    14281 ggtaaagcta caatatatgc taagtgtgga aataaagaaa ttgagagaac atttaggatt
    14341 caagctagag ttgctgaaat aaatatggat gatataaaaa tagaagttga ccagaatgaa
    14401 aaattgaaac cagatataat aacttctcca cctggtttag aaccaccaaa aatatattat
    14461 gagttagtag attcaaaatt acctgtagaa agagcaataa gtttaagtag taatggaaat
    14521 gttgtagggc ttagagaagg taaagatagg gtgagaataa cttgtggaga taaatctaaa
    14581 gtaataacag taacagtagt caaagaaact ataacgaatg actatataca aaacttggtt
    14641 ggttcacata aaatagtaaa caataaagtt attattaaac ttgaatggga ttatatgaaa
    14701 gatgtctttg attatgatat atatcttaaa gataagttgt caggagaatc atttaagaaa
    14761 attaaatcta taactataaa tgaaagtgat ttaggtaagt ctaacaaggt atatgcgact
    14821 atagaagtag atatgaaggg taatcaaaat atagattttg atatttatgt catagggaaa
    14881 aaggattcag atacaactaa gccaagtaat gtaataaata ttaccaatgg aaatggaaat
    14941 tcaaataatg ataataatat taaagatatg agggtagaaa atatatcagc taatgttgat
    15001 agagataata atgtcattag agttaactgg agtagtatat ctcataagga ctgtacttac
    15061 agtatatatg taaaaaataa tactaatgga gatggagaat ttactctata tcaaagtgat
    15121 atacagggaa atgagcatac tataagtatt cctgaaggag atttaaatct agatatttat
    15181 gttgttgcaa attataatgg aaatagctct caaaatagtg atgttataaa tgtaagatag
    15241 atttgtatac aaattgaaag tgttgagttt atttaaatat taaattaaga aaaaggatag
    15301 tatagggtaa ttatactatc cttttttgtt acaatatata ggtttaaaat ctctagtaat
    15361 ataaattata aaaagtctta tttacacaaa tctatagata ttttttttat taatcaatat
    15421 tgtcttaaat tgaacttagt gcagcttcta ttgcatgtat tactgtcgta tcaaatagtg
    15481 gaatagaagt gtcattttgt ttaattaaaa gacctatttc tgtacatcct aatataacag
    15541 cctctgcacc ttgtttgcca agcttatcta taatagataa gaaagctttt tttgaagatt
    15601 cagatataat tcctaagcaa agttcattaa atattgtgtc atttactatt atacggtctt
    15661 cttcatttgg aattaagact tcaattccat tattaataat tacattttta taaaaatctt
    15721 gttccattgt atatttagta cctaataagg ccactttttt tatttctttt tcccttaata
    15781 ccgtagcagt tacatctgct atgtgaagta atggaatatg tatactttct tgtattttat
    15841 ctgatacctt atgcatagta ttagtacaga taacaataaa atctgcccct gcctcttgta
    15901 atttaattgc agcatcagct aaaattttgg cgcttttttc ccaatttcca ctagattggc
    15961 attcttcaat ttcttgaaaa tctacactgt aaagtataca tttagaagag tgtagtccac
    16021 ctaatctttc ttttattact gtattaataa cttgataata tgtaatggta ctttcccaac
    16081 tcataccacc aattaagcct atagtcttca tattatacct ctcttattta tttattttga
    16141 aatagctatt tacaaataca agtatagcat ctaaaataga agcatacaat aataaaattg
    16201 tatggagtac aattataatt aagaggagta attatatgcc aataaattct tttgaaaact
    16261 atcctatgac atggaaacca aaattagata accgaaaacc accaatatat aaaactttag
    16321 caatgttatt agaagaagat attaaaagag gaaatttaaa tcctggagat aaactgcctc
    16381 cacaacggga attagcagat tttctagatt taaacttaag tacaataaca cgagcattta
    16441 aactttgcga agaaaaggga ctaatatgtg caaaagtggg aaagggaaca ttcatttctt
    16501 ctgatgtaaa tgtaagtaat acattattat atcaaacgga atcaaaagac ataattgagt
    16561 tggggacagt tcatcctccg tatgagcaaa atacatatat tattgacttt attaaaaatg
    16621 tactaaaaca accagaaatg gataggtttt tacaatacat gtctccatct ggcacatata
    16681 tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa tgtatacacc agtgaagaaa
    16741 atatattatt atctacagga gggcaaaatg ctatatgtgc aactttacta ggattattta
    16801 aagctggtga tagaattgct acagattctt taagtttttc tggaattaag tcaatagcta
    16861 aaatgatagg tattcaatta gtcccaattc ctcaagaaaa taatgaaata tcgattgaat
    16921 atcttgaaag ttattgtaaa aatgaaaata taaaaggcat atatcttata ccagattatc
    16981 ataatccaac gacacatact atgtctgatt tatctcgtaa aaagatagcc aagatagcaa
    17041 agcaatataa tataataatt attgaagatg ctataaatag catttttaga gacggtatac
    17101 aaactcccat attttcttta gctagtgata atactatata tatttttagt acatctaaat
    17161 ttttatgtgc tggtcttaga gtagcatttg tagttgctcc aaaacgatat atagaaaatt
    17221 tagaaaatgc actatataat atgaatctaa tggtttctcc atttacagca gaaatagtac
    17281 accgtttatt atattctcct attatagata aaattataca agagaaaaga gatgcaatta
    17341 tagaaagaaa tgaagttgct gataaagttt tgtcagatta taatttgatt ggtgataaga
    17401 attgcagttt tcgttggtta cttcttcctg atgaattgga tggaaaatca tttgaaattt
    17461 ctgcaaaaaa tctaggagtt caagtatatt gtgctgaacg attttcagta ggaaactcta
    17521 cagttccaaa agcagttcgt atttgtgtaa cagctccaaa agatgtagaa gaattagaaa
    17581 aagggcttaa tataataaag tcacttctta agagttaaat ttattttact attatttaat
    17641 taaataaata aaaaggcgga gaaatttatt ataataaacc tccgctttta ttatttaatg
    17701 aaatacttta ctttttatta aaaaatttaa ttttaataat cttgaaaata taatcgtttt
    17761 atggtaaaat aattcttaaa aatagaaaaa attaatcatt tgttaaaaaa aatgcataat
    17821 ttattgtaat ttattgacga aggttgaaaa atattcataa atgtgcgaaa taacagaaaa
    17881 aatgcaaaaa ttaattcaat ttcatttgtt ataaaatcta tatggcaaaa ttaaatctgc
    17941 ctatcagaat gatataaaca catcagaaag gaggacgcta tcaaataata caagtagtta
    18001 actttgatag caaaaaatta tgaatgaaca aactagaata tccttagaga gagctgctga
    18061 attaaaatca aaaattgatg attatattca ggctagaaaa acgattaaca gaggtcttga
    18121 aaaagaagaa gagataaata aacgaaaaca gaaaatatta agtatcttaa atggaactga
    18181 agaggattgg aataactaca aatggcaatt atcaaataga ataacagatg tagatacttt
    18241 atcaaaaatt ataactctaa ctaaaaaaga aaaagaatat ataaaagagg ttggtactca
    18301 atttagatgg gcaatatctc catattattt gagtcttata gacccagaag atatatgtga
    18361 cccaataaaa ttactgtcta taccaacaca tatagagttg gaagatgaac aagaagattt
    18421 ggacccaatg ggagaagagt atacaaaccc agcaggatgt ataactagaa gatacccgga
    18481 tagattaata ataaatgtaa caaatgagtg tgctatgtat tgtagacact gtcagagaag
    18541 aagaaatatt ggacaacaag attctcataa gtcaaaagct attatccaag aatctataga
    18601 ctatatcaga gaaaatgaag aaataagaga tgtactagta actggtggag atgctcttac
    18661 attaaaagat gattatttag agtggattct tagccaactt aaagagatac cacatgttga
    18721 ttatgttaga ttaggtacta ggactcttgt tacaatgcca caaagaatta cagatgaatt
    18781 ttgcaatatg ctaaaaaaat atcaccctat atatataaat actcatttta atcatccaat
    18841 ggaaataact aaggaatcta aagaagcttg tgaaaagtta gcaaatgcag gagttccatt
    18901 aggaaatcag gcagtattat taaatggaat aaataatgat aaatttgtaa tgagatgttt
    18961 aaatcaagaa ttactgaaaa taagagtaaa accttattat atattccaaa gtaaacatgt
    19021 aaagggaaca aaacatttca atacatcagt agatgatggt cttgaaatca tggagtattt
    19081 aagaggatat acatcaggaa tggctatacc aacatatata gtaaatgctc caaaaggagg
    19141 aggaaagact cctttgcttc ctcaatacct tgtatcaaaa ggaacagatt acgttatgct
    19201 tagaacatgg gaaggaaaag ttataaaaat ggaagatgaa cctgctgtag atataaagaa
    19261 acttataaaa gaacaagcac aggattaaaa aaatatttaa agtttataaa aagaaacaac
    19321 tttaaaataa aaagaaaatg gatgtcaaat ttatatcaat ttgcatccat tttttattag
    19381 ttatttatta ttttgttgtt gattaactaa ttcaagatat ccacttgggt ggaagtaata
    19441 tatctcagaa gtattttcat ttacaaagta accaatatca ccatctgcat ctggtaaaaa
    19501 tacataacca ttaagatttt tagcttttaa tgcttcaaaa tcactagcat ttccttgata
    19561 tatgtaatca acttcattgt tgtattttac taaaagttct ttagcttgtt cttgagtcaa
    19621 tttagtattt tggcttacag gaacattctt ttgagaatct gcataagaag attcatttac
    19681 tggtgcagtt aataacaaac aagataaaac taataaagat ataagtgctt ttttcatact
    19741 aaaaccctct ttcatgttta attatataag attcattact taataatatt gtatgtttcc
    19801 tataattaaa tattaccatt aaagagaata atattacagt tacacagctg taacttttga
    19861 ttacaaaatc gtaattaaat tgtattttta ttccatttgt attttatcat gtctacgatt
    19921 tacaaactca cagaagtata tctttataac gcccatcaca ggagcagata gaatcatacc
    19981 aggtatacca aagaatcctc caccaacagt aactgctagg attgtaaaga atgggctaag
    20041 acctagttgt ccacctacga tttttggctc tataaatgta acttcaactt gttgaattat
    20101 tattaagtaa attaaagaag ttaaagcaat tgttggattg gagaataaat taattattac
    20161 aactggtgcc atacctatta ctggaccaaa atatggaacc atattcatga cacccataag
    20221 tgtaccaaat aatagtgcat attcagattt taaaaagtaa agaccaattc cagaaagtac
    20281 tccaactata aaagaatcta aaatcttacc tgtaaagtat ttaccaatat ttaaatttaa
    20341 gctttgacat agttctatta taaagtctcc atatttttta cctaaggaaa tataaactac
    20401 tttttttgta aacagtaaga atttttcttt ttctaataat atatagaaac aaattataaa
    20461 acctaaaaga aattgtacta taaattttcc aactgaaaat gtagtgctaa atatcccacc
    20521 aagtgaacca ataagcaagt tactaaattt aggcattaca ctcataattt tgtcaccata
    20581 ctcttttaat gtattagggt caactgattg taaactctta cctaagtcga ataagaattg
    20641 ctctgtttta tctatgtatg taggaatttg agcaactaaa tcagctaaac tatttactat
    20701 ggatggaact gtaaataata taaaggaaat tagtataact atgagtacac cataagtaag
    20761 aagtaacgaa tatattctct taaaatttaa tttgctttct aagaacttta ctagtgggtt
    20821 gaatatatat gctaatacaa atgctattat aaatggtgtt agtagtgaca ttaataaact
    20881 tatcactcca aaaaaatatt tgtaattatc aataacttta atcaagatat atgaaacaac
    20941 tacagtgagt acaatattta gataaatttt tttatcttgc aaaataacac aacctttctt
    21001 atatttatta aatttagata tacttaaatc taagttaaag ttaatattta tagcttatta
    21061 tacaat
//
LOCUS       DAENCV010000061        20701 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAENCV010000061 DAENCV010000000
VERSION     DAENCV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.062"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KQD82_002281"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KQD82_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG4380750.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KQD82_002282"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KQD82_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG4380751.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KQD82_002283"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KQD82_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG4380752.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KQD82_002284"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KQD82_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG4380753.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KQD82_002285"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KQD82_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG4380754.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KQD82_002286"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KQD82_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG4380755.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KQD82_002287"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KQD82_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG4380756.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4711
                     /gene="rpsC"
                     /locus_tag="KQD82_002288"
     CDS             3911..4711
                     /gene="rpsC"
                     /locus_tag="KQD82_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG4380757.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4747..5178
                     /gene="rplP"
                     /locus_tag="KQD82_002289"
     CDS             4747..5178
                     /gene="rplP"
                     /locus_tag="KQD82_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG4380758.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5180..5383
                     /gene="rpmC"
                     /locus_tag="KQD82_002290"
     CDS             5180..5383
                     /gene="rpmC"
                     /locus_tag="KQD82_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG4380759.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5407..5661
                     /gene="rpsQ"
                     /locus_tag="KQD82_002291"
     CDS             5407..5661
                     /gene="rpsQ"
                     /locus_tag="KQD82_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG4380760.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5687..6055
                     /gene="rplN"
                     /locus_tag="KQD82_002292"
     CDS             5687..6055
                     /gene="rplN"
                     /locus_tag="KQD82_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG4380761.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6079..6384
                     /gene="rplX"
                     /locus_tag="KQD82_002293"
     CDS             6079..6384
                     /gene="rplX"
                     /locus_tag="KQD82_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG4380762.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6414..6956
                     /gene="rplE"
                     /locus_tag="KQD82_002294"
     CDS             6414..6956
                     /gene="rplE"
                     /locus_tag="KQD82_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG4380763.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6974..7159
                     /locus_tag="KQD82_002295"
     CDS             6974..7159
                     /locus_tag="KQD82_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG4380764.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7192..7590
                     /gene="rpsH"
                     /locus_tag="KQD82_002296"
     CDS             7192..7590
                     /gene="rpsH"
                     /locus_tag="KQD82_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG4380765.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7621..8163
                     /gene="rplF"
                     /locus_tag="KQD82_002297"
     CDS             7621..8163
                     /gene="rplF"
                     /locus_tag="KQD82_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG4380766.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8199..8567
                     /gene="rplR"
                     /locus_tag="KQD82_002298"
     CDS             8199..8567
                     /gene="rplR"
                     /locus_tag="KQD82_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG4380767.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8588..9097
                     /gene="rpsE"
                     /locus_tag="KQD82_002299"
     CDS             8588..9097
                     /gene="rpsE"
                     /locus_tag="KQD82_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG4380768.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9112..9297
                     /gene="rpmD"
                     /locus_tag="KQD82_002300"
     CDS             9112..9297
                     /gene="rpmD"
                     /locus_tag="KQD82_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG4380769.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9330..9773
                     /gene="rplO"
                     /locus_tag="KQD82_002301"
     CDS             9330..9773
                     /gene="rplO"
                     /locus_tag="KQD82_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG4380770.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9817..11085
                     /gene="secY"
                     /locus_tag="KQD82_002302"
     CDS             9817..11085
                     /gene="secY"
                     /locus_tag="KQD82_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG4380771.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11102..11752
                     /locus_tag="KQD82_002303"
     CDS             11102..11752
                     /locus_tag="KQD82_002303"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG4380772.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11753..12499
                     /gene="map"
                     /locus_tag="KQD82_002304"
     CDS             11753..12499
                     /gene="map"
                     /locus_tag="KQD82_002304"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG4380773.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12521..12790
                     /locus_tag="KQD82_002305"
     CDS             12521..12790
                     /locus_tag="KQD82_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG4380774.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12828..13046
                     /gene="infA"
                     /locus_tag="KQD82_002306"
     CDS             12828..13046
                     /gene="infA"
                     /locus_tag="KQD82_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG4380775.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13082..13195
                     /gene="rpmJ"
                     /locus_tag="KQD82_002307"
     CDS             13082..13195
                     /gene="rpmJ"
                     /locus_tag="KQD82_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG4380776.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13348..13719
                     /gene="rpsM"
                     /locus_tag="KQD82_002308"
     CDS             13348..13719
                     /gene="rpsM"
                     /locus_tag="KQD82_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG4380777.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13755..14153
                     /gene="rpsK"
                     /locus_tag="KQD82_002309"
     CDS             13755..14153
                     /gene="rpsK"
                     /locus_tag="KQD82_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG4380778.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14185..14808
                     /gene="rpsD"
                     /locus_tag="KQD82_002310"
     CDS             14185..14808
                     /gene="rpsD"
                     /locus_tag="KQD82_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG4380779.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14886..15833
                     /locus_tag="KQD82_002311"
     CDS             14886..15833
                     /locus_tag="KQD82_002311"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG4380780.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15854..16195
                     /gene="rplQ"
                     /locus_tag="KQD82_002312"
     CDS             15854..16195
                     /gene="rplQ"
                     /locus_tag="KQD82_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG4380781.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16315..17148
                     /locus_tag="KQD82_002313"
     CDS             16315..17148
                     /locus_tag="KQD82_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG4380782.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17136..18002
                     /locus_tag="KQD82_002314"
     CDS             17136..18002
                     /locus_tag="KQD82_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG4380783.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17996..18799
                     /locus_tag="KQD82_002315"
     CDS             17996..18799
                     /locus_tag="KQD82_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG4380784.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18831..19562
                     /gene="truA"
                     /locus_tag="KQD82_002316"
     CDS             18831..19562
                     /gene="truA"
                     /locus_tag="KQD82_002316"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG4380785.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19682..20113
                     /gene="rplM"
                     /locus_tag="KQD82_002317"
     CDS             19682..20113
                     /gene="rplM"
                     /locus_tag="KQD82_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG4380786.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20142..20534
                     /gene="rpsI"
                     /locus_tag="KQD82_002318"
     CDS             20142..20534
                     /gene="rpsI"
                     /locus_tag="KQD82_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG4380787.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8261 a         2444 c         4317 g         5679 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataga atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaagggg ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgataaca gaggaaacta cagaggacaa agacctcaag gtggatcaag
     4681 acctcaaaga actgaaaaca aaggaaatta ataatagtta ggttcaagga aggaggaaaa
     4741 atactcatgt taatgccaaa aagagtaaag cgtcgtagag ttcatagagg aagcatggct
     4801 gggcaagctc ataagggtaa taaagttact tatggtgagt ttggattagt agcattagaa
     4861 gcttcttgga taacttctaa tcaaatagaa gctgccagaa tcgcgatgac tagatatata
     4921 aaaagagggg gaaaggtttg gataaaaata ttccctcata aaccagtaac aagaaaacca
     4981 gcagagactc gtatgggtgc agggaaaggt tctccagaat attgggtagc tgtagttaag
     5041 ccaggaagag ttatgtttga attagcaggt gtttctgaag ataaagcaag agaagctatg
     5101 agacttgctg cacataaatt accaatcaaa tgtaaatttg ttaaaaaaga agatttagaa
     5161 gtaaagggtg gtgaatagga tgaaagctaa agaattaaga gatttaacaa gcgaagagct
     5221 aatgaacaaa ttaaacgact tcaaaagtga attatttagc ttaagattcc aattagctac
     5281 tggtcaatta gaaaatacag ctagaataaa gtttgttaag aaggatatag caaaagttaa
     5341 aactgttctt gctgaaagaa agttatacga aactagagct taatttggaa aggaggcttt
     5401 ttaaacatgg aaagaggaag aagaaaagtt agaataggcc gtgttgttag tgataagatg
     5461 gataaaacaa tagtagtagc tgttgaagaa ttcgtacgtc atccactata taataaacgt
     5521 gttaagagaa ctaagaaatt caaggctcat gatgaaaaga atatatgtaa catcggagat
     5581 agagttaaaa taatggaaac tagaccatta tctaaagata agagattcag actagttgaa
     5641 gttgttgaga aagttaagta gtttttgaag aaggagggat tacgatatga tacaacaaga
     5701 atcacgtcta agagttgctg ataactcagg agctaaagaa cttttatgta tacgtgttct
     5761 aggcggaagt aaaagaagat atggtaacat aggcgacgtt atagttgcta ctgttaaaag
     5821 tgcaacacct ggtggagttg taaaaaaagg taaggttgtt aaagctgtta tagtaagaag
     5881 caaacaaggc gtaagacgta atgacggaag ctatatatct tttgatgaaa atgctgctgt
     5941 tataataaag gacgataaaa ctccagtagg aactcgtata ttcgggcctg ttgctagaga
     6001 gttaagagac aatgaattca tgaaaatagt ttctcttgct ccagaagtac tataattaag
     6061 gaggtgcaat aggacatgat gcgtgttaaa aaaggtgaca ctgttgtagt tatagcaggt
     6121 aaagataaag gtaaaaaagg ttcagtttta aaggtatatc ctaaaactag caaagtgtta
     6181 gttgaaggtg taaatgtaat aactaaacat caaaaaccaa gtgctatgaa ccaacaaggt
     6241 ggaataataa ataaagaagc cccaatacac atatctaatg taatgccatt tgatcctgaa
     6301 acaggaaaag gcgttagagt aagatatgaa gtaaaagatg ggaacaaagt aagagtatca
     6361 gcaaagagcg gaaaagaatt ataataaata ttgcttgaaa ggagggacct taaatggctt
     6421 ctagattaca agaaaaatac atgaaagaag ttgctcctgc tttaatggag aaatttggat
     6481 acaaaaacgt aatggagata cctaagttaa ataaaatagt tattaacatg ggtataggtg
     6541 acgctagaga aaatccaaaa ggattagaaa aaggcgttga agaattagaa atgatatcag
     6601 gacaaaagcc tgttataact aaagctagaa aatctgttgc taacttcaaa ttaagagaag
     6661 gaatgccaat aggaacaaaa gttacattaa gagctgacaa aatgttctac tttatggaca
     6721 aattagtttc agtttcttta ccaagagtta gagactttag aggagttaac cctaatgctt
     6781 ttgatggtag aggaaattat gctttaggag ttaaagaaca attaatattc cctgaaatag
     6841 aatatgataa aatagataaa gtaagaggaa tggatataat atttgttact acagcaaaaa
     6901 ctgacgaaga agctcgtgaa ttattaaaat tattaggaat gccattttct aagtaagcaa
     6961 aggagggatt cccgtggcta gaaaagcgat ggttgttaag caacaaagaa agcaaaagta
     7021 cgctactaga gaatacacta gatgtactat atgcggaaga ccgcattcag ttttgaaaaa
     7081 atttggtata tgccgtatat gctttagaga attagcttat aaaggtcaaa tacctggtgt
     7141 tagaaaagca agctggtaga agtttgatta gaatggaagg agggcaacaa aatgacaatg
     7201 acagatccaa ttgcagatat gcttactcgt ataagaaatg ctaatgttgt taagcatgaa
     7261 actgtagatg ttccagcttc taatatgaag aaagaattag ctagaatctt attagaagaa
     7321 ggtttcataa gaggttatga tgttatagaa gatggaaaac aaggtatcat aagaatacaa
     7381 ctaaagtacg gacaagaagg cgaaagagtt ataacaggtc ttaagaaaat atctaagcct
     7441 ggaatgagag tttacgctgc taaccatgag ataccaaaag tattaaacgg attaggaata
     7501 tcagttatat ctacttcaaa aggaatatta actgacaagc aagctagaaa agaaaatgtt
     7561 ggtggagaag taatctgcta cgtttggtaa ttaatatgaa caatgtaagg aggtgcgacc
     7621 atgtcaagaa taggtgttaa gccaataata ataccagcag gtgttgaagt tactatagct
     7681 gaaggaaatt tagttacagt aaaaggtcca aaaggaactt taactaaaca attaagtgct
     7741 gagttaaaca taaaaaaaga agaaaataca ataatggttg aaagaccaac tgataataaa
     7801 aaacatagat ctttacacgg actaactaga actttattag ataatatggt tgtaggtgta
     7861 aatactggtt tcgaaaagaa attagaatta aaaggtgttg gatatagagc tcaaaaacaa
     7921 ggaaagaaat tagttatgaa cttaggtttc tctcatccag ttgaaatgga agatccagaa
     7981 ggaataactg ttgaagctcc aaaccaaaca gagttaatag taaaaggtat agacaagcaa
     8041 ttagtaggga attatgctgc taagataaga gcttggagaa agccagagcc atacaaaggt
     8101 aaaggtataa aatacgttga tgaagtaatc agacgtaaag aaggaaaaac tggtaaaaaa
     8161 taataccaaa acttaaacgc tagaaaggag tgagctaggt gttaaaaaag gctgataaaa
     8221 atgctaatag acttcaaaga cataagagag ttcgtagaaa aataagcggt acttctcaaa
     8281 gaccaagatt atgtgtattt agaagtgcta acaatatata tgctcaaata atagatgaca
     8341 caaaaagagt aactctagtt gctgcatctt ctttagaagc agaagttaag agtgctgtca
     8401 atcatacagg aaacaaggaa gcagctaaga aagttggaga attagttgct aaaaaagctg
     8461 ttgaaaaagg aatcactgaa gttgtatttg acagaggtgg atacttatat catggaagaa
     8521 ttcaagaatt agctgaaggt gctagagaag ctggtcttaa gttctaatac gaaggaggga
     8581 aaaatacatg ctacgtcgta agccgataga tgcaggacaa cttgacttac aagaaaaagt
     8641 tgttgaagta agacgtgtta ctaaggttgt aaaaggtggt agaaatttca gatttgcagc
     8701 attagtagtt gttggagatg aaaacggaca cgttggtata ggtgctggga aagcaatgga
     8761 agtaccagac gctataaaaa aagcagttga agatgctaag aaaaatctaa tagtagtgcc
     8821 aatagttggg actacaattc ctcacgaagt acgcggacac tttggtgctg gaaatatatt
     8881 aataatgcct gctgtagaag gtacaggagt aatagctggg ggacctgcta gagccgtact
     8941 tgagctagct ggactaaaag acgttagagc taaatcttta gggtctaaca atccaagaaa
     9001 tatggtaaat gctacaatag aaggacttaa ttctctaaaa acagttgaag atatagctaa
     9061 acttagaggt aaaaaagttg aagaacttct agggtaagga ggtagtgtaa aatggctaaa
     9121 ttacaaataa agttagttag aagtgttata ggaactactc ctaaccaaaa aaagaatgtt
     9181 gaagcgttag gattaagaaa aagagaacaa gttgttgtaa aagaagacaa tgctcaaaca
     9241 agaggtatga taaataaagt aagtcactta ttagaagtaa ctgaaatagc tgaataattt
     9301 ttataaagta gtgcaaggag gtgcaatcca tgaagttaca tgagttaaaa cctgctgaag
     9361 gtgcagtaag agctaagaga agattaggta gaggtactgc aactggacaa ggtaaaactg
     9421 caggacgtgg acaaaaaggt caatggtctc gttctggtgg tggagtaaga gtaggatttg
     9481 aaggtggaca aatgccacta gctagaagac ttcctaagag aggtttcaat aacatattta
     9541 agaaagttta cacagaagtt aatgttgaag ttttaaacag atttgaaaat ggaacagaaa
     9601 taactgcaga attattaaaa tctactaaaa ctataagcaa aataggtaaa gacggaatta
     9661 aaatcttagg tgaaggtaat ttagagaaag ctttaactgt taaagctgct aaatttacag
     9721 cttcagctca agaaaaaata gaaaaagctg gtggaaaagc agagttagta taataacctg
     9781 ctaatctcct agccagtaca ggcggggtga attagcgtgc tgtcaaaatt aaaacaagct
     9841 tggaagatta aagcagtaag aaaaaaagta atgtatacac ttatgatgat cgtgatattc
     9901 agaataggta ctactatacc agttccaggc atagacacta gtattataca aaagatggta
     9961 ggcggcaata gtctactttc tctgtataat atgtttactg gtggagcatt tagtaacttt
    10021 tcactatttg ctttaggtat aagtccatat atcactgcat caatcataat acaacttcta
    10081 acagtaggtt ttgaaagttt agcagaactt caaaaatctg gtgaagaagg aaagaaaaaa
    10141 atcaataagt acactaaata tacagcacta gctctagcag ttgtacaagc tttgggaatt
    10201 acattaggaa ttgtaagaag tgctttaata tcaaatagtg tgttttttat cacaactgtt
    10261 gtaataacat tagtttcagc aagtatgtta gtaatgtgga ttggggacaa aattactgaa
    10321 aaaggaatcg gtaatggtag ttcagtaata atatttgcag gtataatatc aagaatacct
    10381 acagatgtta ttaaaatatc gcaacaagtt aaatctggcg aagttgcacc ttgggttata
    10441 gtgattctag ctgtagttat attacttaca gtaacaggag ttacatttat acaagaagca
    10501 actagaaaga tacctgtaca atatgctaaa agagttgttg gaagaaaaat gtatggagga
    10561 caaagttctc atataccaat gaaagttaac cagtcaggag ttatgcctat aatttttgct
    10621 agttcacttt tagctttccc tcaaacaata gctatgttta tgggtccaaa tgctcaggct
    10681 tttgttcaaa aatacttaag tatggcaaca gaacaaggat tttggacata tagatcaatt
    10741 gagattctac ttataatttt cttctcatat ttttatacaa ctgtatcatt taatacagaa
    10801 gacatatcta aaaatatgaa aaataatggt ggatttatac caggtataag accaggtgag
    10861 cctactatgg attatttaaa tagaatttta actagattaa ctttagctgg agctactttc
    10921 ttagcaataa ttgctatggt gccagctctt acaacacatt atatgaaagt aaatatgagt
    10981 ctagctggga cttcattgct aatcgttgtt ggagttgctt tggagcttaa gagacagtta
    11041 gagtcaaact tagttatgag aagctatcaa ggtttcttaa aataaaatgg agatggataa
    11101 tatgagaata atattacttg gacctccagg tgctggtaaa ggtactcagg cggcaggaat
    11161 agtagaaaaa tacaatatac ctcatatatc aacaggagat atattcagaa agaatataaa
    11221 agagggaaca gaacttggaa aaaaagctaa agaatacatg gaccaaggtt tattagtacc
    11281 agatgagtta actgtaggtt tagttactga tagaatatct caagaagatt gtaaaaatgg
    11341 atttatgtta gatggatttc caagaaatgt agcacaagga gaacatttag atatcttctt
    11401 aaaaaatgct ggtatatcac tagataaagt tgtcaatatt gaagttgata agagtatatt
    11461 agtgtctaga gcagttggta gaagaatatg taagtcttgt ggagctactt accatgttga
    11521 gtttaatcct cctaaagtag aaggtgtatg tgatgtatgc caaggagaat tatatcaaag
    11581 agctgatgat aatgaagaaa ctgtatctaa gagaatacaa gtttatctag atgaaactaa
    11641 gcctttagta gattattata gcaaacaagg tataatagca gatataaaag gtgatcaagc
    11701 aatagataaa gtatttgaag atattgtcgc agccttagga agtggaaaat aaatgattat
    11761 cttgaaatca aaaaaagaaa tagaactttt aagagaagca ggtaaaatag ttgctgacac
    11821 tcatgaagtt ttaagaaaag ctatttctcc gggaatatct actttagaat tagataaaat
    11881 agctgaagag aatataagaa aatacaatgc agaaccttct tttaaaggat atggaggttt
    11941 tccaggaagt atatgtgctt ctatcaatag agaggtagta catggtattc caggagaaac
    12001 tatattacaa gaaggtgaca ttgtaagttt ggatatagga gcctattata aaggatatca
    12061 tggtgattct gcgaaaactc acggagtagg catgatatct gaagaagata ggaaattgat
    12121 agaagtaact agagaaagct tctatgaagg aataaaattt gctaaattag gatatagact
    12181 ttcagatatc tcacacgcag tacaaacaca cgttgagaaa catggtttct cggtagttag
    12241 agatttagtg ggtcatggag ttggagcaaa tcttcatgaa gacccacaag tacctaatta
    12301 tggactccca ggaaaaggac caagacttag agaaggaatg gttattgcta tagagccaat
    12361 ggttaatgca ggtcgttatc atgtaaagac tctatcagat gggtggacga ctgttacaat
    12421 agatggaaaa aaatcagctc attatgagca tacgatagca attactgaac atgaaccttt
    12481 gatattgaca aagttgtaat ttaaaaagta ggtgaaaaaa gtgctatcag ataatttaag
    12541 tataggtcaa gttgttaaag tttcattagg acgagataaa ggaaatttat tctttgtagt
    12601 caaaataatt aataatgaat atgtattaat agctgatgga aaaaagagaa aacttgacaa
    12661 acctaagtta aagaaagtga agcacttaaa aaaatataat tttattaatg atgaagttag
    12721 aaaaagagta gtatctggac aagagattac agattctttt ttaagagctg aacttactaa
    12781 gttaaattag ataatttaac ttattgaaat ggagggctgg ttagttaatg gccaaaaaag
    12841 atgttataga attagaaggt acagtttcag aagctttacc taatgctatg ttcaaagtta
    12901 aactagaaaa tggacatgag atattatgcc acatttctgg aaagctaaga atgaacttca
    12961 taagaattct tgaaggtgac aaggtgaatg ttgaactttc tccatatgac cttacaagag
    13021 gaagaattac ttggcgtaag aagtagacta tattaatatt tagtctaagg agggattaat
    13081 aatgaaggtt agaccatcag taaaaccaat atgtgaaaaa tgtaaagtaa taaaaagaaa
    13141 aggaaaagta atggttatct gtgaaaatcc aaagcataag caaaagcaag gataagaaat
    13201 tattactagc attttgttaa attttatagt ataatactat attgtgatgt taacaaaaga
    13261 acgttaaaat cgttgaacaa tctggcgtag gattggcata tgatgtgaag ttttaaacac
    13321 taaaaactat gtgtaggagg tgcgatcatg gcaagaatag ctggggtaga tttacctaga
    13381 gaaaaaagag cggagatagg cttaacttat atatatggta taggtaaagc aactgctaac
    13441 gaaatattag ctaaggctga gataaatcca gatactagaa taaaagattt atcagaagat
    13501 caagttaatg agttaagaaa agttatagat gacgatttct tagttgaagg tgacttaaga
    13561 agagaaattg ctttaaacat aaagagatta agagatataa aatgttatag aggaataaga
    13621 cacgctaaag gtcttccatt aagaggacaa agaactaaga ctaatgctag aactagaaaa
    13681 ggtcctagaa aaacagtatc tcgtaaaaag aaaaaataaa taaataataa ctagatgaag
    13741 gagggaaaag gaaaatggct aaaccaaaaa agaaagttac acgtattaga agaagagaac
    13801 gtaaaaatat agaacgtggt catgctcata tacaatcaac tttcaataat acaataataa
    13861 ctttaactga cgttcacgga aatgctatat cttgggcaag ttctggacaa ttaggattca
    13921 aaggatcaag aaaatcaact ccatttgcat ctcaaatggc tgctgaaaca gctgcaaaag
    13981 ctgcgatgga acacggacta aaaagtgttg aggtattcgt aaaggggcca ggttcaggaa
    14041 gagaagctgc aataagagct ttacaagcaa ctggactaga agtaactatg ataaaagacg
    14101 ttactccaat cccacataac ggatgcagac caccaaagag aagaagagtg taattaataa
    14161 gagtacaata ggaggtgtag ataaatggca agatatacag gtgcatcatg tagacaatgc
    14221 cgtagagagg gaatgaaatt attccttaaa ggtgatagat gttatacaga caaatgtgct
    14281 atagtaaaaa gaaactatgc tccaggtcaa catggccaag gaagaaagaa agtttctaac
    14341 tatggattac aattaagaga aaaacaaaaa gttaaaagaa tatatggagt tttagagact
    14401 caatttagaa atttatatga acgtgctgaa aatatgcctg gtaaagcagg tgagaactta
    14461 ttaagtttat tagagagaag attagataat gtagtttaca gaatgggact agcatcttct
    14521 agaaaagaag ctagacaatt agtgactcat ggtcatttca ctttaaatgg aaataaagta
    14581 gacatacctt cattaatagt taaggttgga gatgttatag aagttaaaga gaaatcaaga
    14641 tcttctgcaa aattcaaaaa tttagtagaa gttaactcaa gaatagctcc taagtggtta
    14701 gaagctaatg tagaaggaat gacagcaaaa gtagttggtg ttccaacaag agaagatata
    14761 gatcttgaga tagcagagca cttaatcata gaactttact ctaaataata agcacacttt
    14821 taaaaattat tttaaaattt tgtttaccct cagtggatta ctgaatttta aggagggttt
    14881 tgtccatgat agaaatagaa aagccaaaag tagatatagt tgagcttagc gaagactata
    14941 gatatggtaa gtttgttata gagcctctag aaagaggata tggaataact ataggtaacg
    15001 cattaagaag aatattatta tcatcattac caggtgtagc agtgaatgct ataaaaatag
    15061 atggagttct tcatgaattt tcaacaatac ctggtgttaa agaagatgtt actgagataa
    15121 tattaacttt aaaagagctt tcagcaacta tagatggtga aggaagtaga acacttaaaa
    15181 tagaagctca aggaccatgc tctatcacag gtgcagatat aatctgtcct ccagatgttg
    15241 aaatattaag taaagattta gcaatagcta cattagatga taatgctaaa cttaatatgg
    15301 agatatttgt agataaaggt agaggttatg tttctgctga agaaaataaa acagagaatg
    15361 ttccaatagg tgttttacct gtagattcaa tatatactcc tgttgagaag gttagttatc
    15421 atgtggaaaa cacaagagtt ggtcaaaaaa cagattatga taaattagta ctagaagttt
    15481 ggactaatgg tagtataaat cctcaagagg gaatatcatt agctgcaaag gtattagttg
    15541 agcatttaaa cttatttata gacttaactg aacatgtaag tagtgttgaa atcatggtag
    15601 aaaaagaaga agatcaaaaa gaaaaagttc ttgaaatgac tatagaagaa ttagatttat
    15661 cagttagatc atataactgt ttaaagagag cgggaattaa tacagttgaa gaattagcta
    15721 ataaatctga ggatgatatg atgaaggtta gaaacctagg taagaagtca ttagaagagg
    15781 taatacaaaa actagaagaa cttggattgg gattaaaacc aagtgaagaa tagcgagcaa
    15841 aggagggata agaatggcta agtaccgtaa attaggacgt gaaacagctc acagaaacct
    15901 tatgttaaga aacttagtaa cttgcttact aagaagtgga agaatagaaa ctacagtgac
    15961 tagagcgaaa gaaactcgta gaatggctga aaagatgata actcttgcta agagaggaga
    16021 tcttcatgct agaagacaag ttttagctta tgttatggat gaaacagtag ttaataactt
    16081 attcacagat ttagctccaa aatatgctga gagaaatggt ggatacacta gaataataaa
    16141 aatagggcca agaaaaggcg atgctgctga aatggctttt atagaattag tatagtaagg
    16201 aaaggattag gcttgatggc ttaatctttt ttttattttt ttgactaagg tatgatataa
    16261 taatgtgtaa aatgattttt ataagttatt gcttactaaa aggagtatga gttaatggat
    16321 aatatagtaa aggtaaataa tatttcgttt gagtatatta cagatgaagc aaaacttaaa
    16381 gcaatagata atttaagcct agatgttaaa aaaggagaat ttgttgcgat aataggacat
    16441 aatggttctg gtaagtctac tttatctaaa aacttaaatg ctattcttat gcctactgaa
    16501 ggaaatattc ttattgatga tatggatact aaagaagaag aaagattatg ggatataaga
    16561 caaactgcag gcatggtatt tcaaaatcca gataatcaaa tagttgctac tatagtagaa
    16621 gaagacgttg catttggacc tgagaactta ggtatagaac caaaagaaat aagacgaatt
    16681 gtagaggagt ctttaaaaag tgtaggtatg tatgatttaa gagatagaca gcctcatttg
    16741 ttatcaggag gtcaaaagca aagagttgca atagcaggaa ttattgctat gaggcctaaa
    16801 tgtataatat ttgatgaagc aacagcaatg ttagatccat ctggaagaaa agaagttatg
    16861 aaaacaataa aaagacttaa taaggaagaa aatataactg ttatacatat aacgcacttt
    16921 atggaagaag cagtagaagc tgatagagtt gtagttatgg aaaaaggaaa gaaaatctta
    16981 gagggaactc ctagagaagt attcagtaaa ataaagatgt taaaggaaat cggtttagat
    17041 gtaccttgta tgacagagtt atctagttta ttaatagaag aaggtataaa tattagtagt
    17101 gatatattaa ctgtggatga gatggtgatg gaattatgtc aattatagta aagaatttaa
    17161 cacatatata taatgaaggg atgccttttg caagtaaggc acttgatgat gtttcttttg
    17221 agattaaaga tagagatttt gttggtctta taggtcatac aggctcagga aaatctacat
    17281 taatacaaca tttaaatgga ttactaaagc cttcctcagg agaaatattt ataaatgatt
    17341 ttaatataac agataaaaac ttgaatttaa ctgagattag aaaaagagta ggggttgtat
    17401 ttcaatatcc agaataccaa ttgtttgaag aaacgattga taaagatata gcttttggac
    17461 cctctaattt aggcttagag gagtctgaga tacataacag ggtaaaagca tcaatggaag
    17521 ctgtaggact tgattatgag ggatttaaag acaaatctcc ttttgaatta tctgggggac
    17581 aaaagcgtag agtagcaatt gctggagtta tagccatgaa cccagaagtt ctcatattgg
    17641 atgaaccaac tgctggtctt gaccctggtg gaagagatga aatttttaac ttaataaaag
    17701 acttacatga aaaaaagaat atgactataa tactatcatc acatagtatg gatgatatgg
    17761 caaagttagc aaaaacttta attgttatga atcatggtag cgtagaattt atgggaactc
    17821 ctagagaagt atttaaatca aatgctagta aattaaaaga tataggatta gatattccac
    17881 aagttttaga gcttgcattg aaattgagag aaaaaggctt tgacatcagt gaggatatat
    17941 tgactttaga agaagcaaaa caggaaatat taaaagttgt gagaggacga ggattatgtt
    18001 aaaagatata actatagggc aatactaccc aacaagttct gctattcata aattagatcc
    18061 aaggataaag cttgtagcga caattgtatt tatggtatct atatttgtag taaataaatt
    18121 ttggccttat atagtagttt tattatgttt attagctatg ataaaattgg ctaatatacc
    18181 agtcaaatat attgttaagg gtgttaaacc tcttaaatgg attattttgt ttacatttct
    18241 tataaatatt tttttcttac caggtgatga aatatggtca tttggatttt tagctattac
    18301 aaaacagggt ctaagacaag caatttttat ggcaataaga ttaatatttt tagtagttgg
    18361 tacatcattg cttactttaa caacttcacc aatagaatta actgatggaa tagagagatt
    18421 gttgaatcca tttaagaaaa ttggtttgcc agttcatgaa cttgcaatga tgatgacaat
    18481 tgctttacgt tttattccta cattgttaga tgagacagac aaaataatga aggctcagat
    18541 gtctagaggt gcagattttg aaagtaagaa tcttataaat agagcaaaga atttagtacc
    18601 acttttggta cctttatttg taagtgcatt tagaagagct gatgaattgg ctatggccat
    18661 ggaggccaga tgttacagag gtggacataa taggactaag atgagagagt ctgttatatc
    18721 aagaagggat tatatggcat gtgtatttca ggttgtttat ctgggagcaa ttatagctac
    18781 tagatttata gcaatttaat taataagtta tagatataat gaggtaaagt atgagaaata
    18841 taaaaattaa aatccaatac aatggtaaga attattgtgg atggcaaaag caacctgatt
    18901 ctttagggat acaaggtaca attgaaaggg ctatatatga tataacaaaa gaagaaacca
    18961 gtcttatagg gtctggtaga actgattcag gagttcatgc tattggtcag attgcaaatt
    19021 ttaaaattaa ttcaggtata agtatagaaa gtataccaat ggcactaaat gccaagttgc
    19081 caaaagacat atcagttata gaagcatgtg aagtaaatga tgattttcac tccagataca
    19141 gtgcaaaagg taaaacatat aaatatttag tatataatag caagtttaga aatccaatac
    19201 ttagtgagat atcatatcaa gtaaaatatg aacttgattt tgataaaatg tgttcagaag
    19261 caaaaagttt attaggaaca catgatttta aaggttttat gagttctggt tcatctgtaa
    19321 aagatacagt tagaacgata tatgatattg atataagtaa aaaagatgac ttgattactt
    19381 ttgagattag tggtaatggt tttttatata acatggtgag gattattgtt ggaacacttg
    19441 ttgatatggg tcgtggcaga attaacgaac catttttaga tataatacaa tcaaaaacta
    19501 ggtcaagatg tggacataca gctccagcac aaggattatt tctcaaaaaa gttcattatt
    19561 aacttgacac accaggctac atgtattaaa atatatgaga atgtgttaat aagtccactt
    19621 gccccggact tgacataaac ggtaaaaaaa ttttatgaaa agtagttaag gagggacagt
    19681 tatgaaaagt tatatagcta agccagctga tgtacaaaga aaatggtact tagttgatgc
    19741 tgaaggaaaa acattaggtc gtttagcaac agaaattgcg acagtattga gaggtaaaca
    19801 taaaccaaca tttactcctc acgttgatgg tggagatttt gttgttgtag taaatgcaga
    19861 aaaaatagtt ttaagtggta agaaattaga tcaaaaatat tacagatacc atactggtta
    19921 tgtaggtgga ttaaaagaaa tatcttatag agatatgatg gataagaaac cagaagaagt
    19981 tatatcacat gctgtaagcg gtatgttacc taaaaataaa ttaagaagca gaatgatgac
    20041 tagattaaga gtatttgcag gtgctgagca cactcatgca gctcaaaatc cagaagtttt
    20101 aaactttaaa taagattaat aggtgaaagg aggagtttat aatggctaac gttcaatatt
    20161 atggaactgg aagaagaaaa agttctgttg ctagagtaag actagttgca ggtgaaggaa
    20221 atatattagt aaatggaaga gcattagaaa attattttaa ttatgagaca ttaataagag
    20281 atgttaaaca accattagtt ttaactggta atgaaaataa atatgatgtt atagtaaaag
    20341 ttgaaggtgg aggattcact ggacaagctg gagctataag acatggaata tctagagctt
    20401 tattaaaagc tgatttagat ttaagacctg ctttaaagaa agaaggtttc ttaactagag
    20461 atgcaagaat gaaggaaaga aagaaatacg gattaaaagc agcaagaaga gctccacaat
    20521 tctcaaaaag ataattactt atttttttgg aaaagcaaaa tcttttattt actgttattc
    20581 agaaagtatt ggtattttaa aaatgttggg ttaagttaat tattgatata taataaaata
    20641 ataagatatc ttattaggat gtttttcatt aagaagaaca tcctttttat tttgactatt
    20701 t
//
LOCUS       DAENCV010000062        20367 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAENCV010000062 DAENCV010000000
VERSION     DAENCV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.063"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(198..1589)
                     /locus_tag="KQD82_002319"
     CDS             complement(198..1589)
                     /locus_tag="KQD82_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG4380788.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKCP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1600..2820)
                     /locus_tag="KQD82_002320"
     CDS             complement(1600..2820)
                     /locus_tag="KQD82_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG4380789.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2834..3622)
                     /locus_tag="KQD82_002321"
     CDS             complement(2834..3622)
                     /locus_tag="KQD82_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG4380790.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3639..4913)
                     /gene="larA"
                     /locus_tag="KQD82_002322"
     CDS             complement(3639..4913)
                     /gene="larA"
                     /locus_tag="KQD82_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG4380791.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5031..5744)
                     /locus_tag="KQD82_002323"
     CDS             complement(5031..5744)
                     /locus_tag="KQD82_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG4380792.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     DKAKEHMDFISKNYRKNEN"
     gene            5869..7128
                     /locus_tag="KQD82_002324"
     CDS             5869..7128
                     /locus_tag="KQD82_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG4380793.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTIIQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7396..7962
                     /locus_tag="KQD82_002325"
     CDS             7396..7962
                     /locus_tag="KQD82_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4380794.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8163..9368)
                     /locus_tag="KQD82_002326"
     CDS             complement(8163..9368)
                     /locus_tag="KQD82_002326"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG4380795.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9471..9577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9669..10037)
                     /locus_tag="KQD82_002327"
     CDS             complement(9669..10037)
                     /locus_tag="KQD82_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG4380796.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10089..11366)
                     /gene="obgE"
                     /locus_tag="KQD82_002328"
     CDS             complement(10089..11366)
                     /gene="obgE"
                     /locus_tag="KQD82_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG4380797.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11477..11767)
                     /gene="rpmA"
                     /locus_tag="KQD82_002329"
     CDS             complement(11477..11767)
                     /gene="rpmA"
                     /locus_tag="KQD82_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG4380798.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11781..12098)
                     /locus_tag="KQD82_002330"
     CDS             complement(11781..12098)
                     /locus_tag="KQD82_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG4380799.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12113..12424)
                     /gene="rplU"
                     /locus_tag="KQD82_002331"
     CDS             complement(12113..12424)
                     /gene="rplU"
                     /locus_tag="KQD82_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG4380800.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12525..13946)
                     /locus_tag="KQD82_002332"
     CDS             complement(12525..13946)
                     /locus_tag="KQD82_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG4380801.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13964..14665)
                     /locus_tag="KQD82_002333"
     CDS             complement(13964..14665)
                     /locus_tag="KQD82_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG4380802.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14667..16511)
                     /locus_tag="KQD82_002334"
     CDS             complement(14667..16511)
                     /locus_tag="KQD82_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG4380803.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16830..18023
                     /locus_tag="KQD82_002335"
     CDS             16830..18023
                     /locus_tag="KQD82_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG4380804.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18235..19056)
                     /locus_tag="KQD82_002336"
                     /pseudo
     CDS             complement(18235..19056)
                     /locus_tag="KQD82_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(19186..19977)
                     /locus_tag="KQD82_002337"
     CDS             complement(19186..19977)
                     /locus_tag="KQD82_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380805.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(19981..20310)
                     /locus_tag="KQD82_002338"
     CDS             complement(19981..20310)
                     /locus_tag="KQD82_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380806.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6399 a         3703 c         2256 g         8009 t
ORIGIN      
        1 tcttacttta tttatgtaca catgctttta ggatgacaac atcaacttaa atattatatt
       61 actttctaaa tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcagattt
      121 aaaagtaaag tttaaaatat tatatttaaa tctaatttaa tattttttgt tttgtaaata
      181 ttattaaatt aatatttcta atatattttc cctggattta aaatattttt agggtcaaaa
      241 gctaacttta tatttttaat taacatcata taagcatctg aatttgattc atgtaagtac
      301 tcttttttag catatcctat gccatgttca ccagaaactt gaccactaag ttctctagat
      361 tttttataca tatagtcaaa agtttctttt aatcttattt tccatgtatt atcatccata
      421 ccatctttta aaatatatat gtgtagattt ccatctcctg catgaccaaa actttttatt
      481 cttattttta atttgtcttg aagctcgtga gtgtatctta taaactctgc tatcttatct
      541 cttggaacaa cgacatcaca ttcatccatt tgtgtcgtag atgcctttat agcttctaaa
      601 aatgcacctc tggctgacca tatagaatcg tttctttctt gtgtatctga tataaataca
      661 tctaatgcac cattttctaa acataaatta gctacttttt catattcttt ttctatatct
      721 tcagtgctat ttccatcaaa tgtaagcaaa agataagcat ctgatgtatt atctggaaac
      781 tttttaccta aaaattcttc tgctgccaat attacatctc tttccataaa ttctatagcc
      841 gttggtattg attttgattt tattatttta ggaacagttt ctattgccat tgataaatct
      901 ggaaatggta taagcaaact tatacttttc tttggtaaag gaagcaattt taatatagct
      961 ttagtaacta ttcctaaagt accttctgag cccactaata aatcctttat actatatcca
     1021 gaactatttt taaccacctt tccacctaca tttataattt ctccatttgg tagtacaact
     1081 tcaagacctc ttacataatc ccttgtaacg ccatatttta ctgctctcat accacctgca
     1141 ttagtgttta tattacctgc aattgtagca cttttttcac ctgggtcagg tgggtaaaat
     1201 aaatctctat cttgaacata ttgacctatt gtcatcaata aaacacctgg ttctactgtc
     1261 aatgttaaat tttcataatc aacttcaagt attttattca ttttacatag attaatcatt
     1321 attccaccat ttatggcaac agcagcacct acaagaccag taccttgacc tcttggtgtg
     1381 actggtatat tatttttata tgcgtatttc attattttag atacttgttc agtttcaagt
     1441 acatttacta aaacttcagg gcacttctca atccctccaa gttcatcatg tgaaaaatct
     1501 tcatttatat cacttccaac taatacattt tcttctccac aagtatctat taaaaaatct
     1561 atatcttttt tatcgattaa tttatacatt tctatccccc tatttagctg ctgatgccat
     1621 atatttttga ttattatctt tgttattttc tattttttca atcaatttag gaataatatc
     1681 ataaatatca cctattaaag ctaaatgtgc tacatcaagt ataggcgctt tatcatcttt
     1741 atttattgct actatatagt cagcaccctg catccccgca acaaattgaa cagctccaga
     1801 tacaccacaa gttattatca atttaggttt tacagttcta ccactaagac ctatttgttt
     1861 tttagcatct atccaacctg cttctattac aggtcttgtt cctgccactt gagcgtttaa
     1921 cttatcagct aatttatata tcatctccat atcttcttgt ttttttattg ctctacttgc
     1981 cacaactatc acttcagctt cttccagacc aacttcttta tttttttctt ttacactcag
     2041 taccttaatt ttagatttta gttctttttt ttctacttta cataaagtta tctttcctgt
     2101 agtattctct attttttctg gtgctgaaaa tattttatat cttacagtgg caaattgtgg
     2161 tctattatta ggtgtgttta tatgagccat tatatttcca ccaaatgctg gtcttatttg
     2221 gtctaaatca gtatttgatt gaatatcaag tattgtacaa tctgctgtaa gacctgttct
     2281 aaatctagcg gcaagtcttg gagctaatga cctacctaat gtagttccac caactaatac
     2341 tatcgttggt tttattttat ctataaagtt ttctattgct tttgaatatg gttctatcct
     2401 aaaatcttta aattcatctt catcatatac aaatacttca tccaccccat aagaaatcaa
     2461 attactgcac ttatctttta tgtcttttcc tacaaataca caatacacag gttgttttat
     2521 ttttgatgcc atctcacgag ctttacctat aagctcgtac gttacaggat gtatatttcc
     2581 attatgatgt tcaacatata ctgcaattcc cctccattca tctttattaa tttgtacttt
     2641 actactctct ataaattcaa atgcgccttt tggtccactt ttgacacaca acttgcacat
     2701 cttacagcca gcacttattt ctactatacc tgacttttct tctatagccc caaaaggaca
     2761 tatttctaaa attctcttta agtcagttat tttatcttga tttacaacta tctttgacat
     2821 ttattactcc cccttacacc aactttaatt cctctaattt atttacaagt gcaaaactta
     2881 gttcatctgc atttcctctt acaatatttt tatctgtatt tggctttgga ggaaatattc
     2941 ttacaacttg agtaggagaa ccattaagtc catagttatt ttcatcttta tcttcaaagt
     3001 catctaagct atatacaggt atttctctat ctttagtatt aagttttaat ttaaatgaag
     3061 gtagtcttgg ttgaaaaatg tctttttcta cagttattaa acatggatat tcaatctcac
     3121 atacttgtaa atcatttggc atatctattt caacaactat actttcatct ttaacttcaa
     3181 ttaattttgt aacgttagta acatgaggta tatctaaaaa ttcagctact tcaggtccca
     3241 cctgtgctgt atcaccatca gtagtttgtt ttccacaaat tattaggtct acctttccca
     3301 tcttcttaat accttgtgat atggtatagg aagtagccaa tacatctgct cctccaaatc
     3361 tcctatcact tattaatgct ccttcatcag ctcccatcat aaaactttct tcaattacct
     3421 tctttgcctg aggaggaccc atgctaagta cttttagatt cgctttcttc tcttccttta
     3481 ttctaagagc tgtttctaaa gcatataggt catagggatt catttttgaa tctactccat
     3541 ctctttttag tactcctgta gtttcatcaa cttctactga agtcgttcca ggaacttgtt
     3601 taatacatgt caaaatttcc ataaagtaca ctcctttttt atttaactat aactgatact
     3661 ccatcaggta taactactac tttgctctcc ttaccaacta tactattcgc catttctaaa
     3721 gcttctttaa agttaaatgc atgtttcata tgcatttttt ttatcatttc aggattacac
     3781 ttatctgtta ccattatcac agtatattta gataatattc tcgctagtat ctgaaattcc
     3841 cattggtctg gaatcgtatc tactctatcg acacttctaa ttttatctaa aagttgtttt
     3901 ggagaatctg catttgctac attttcataa aaagattgac ctccatgacc atcattacat
     3961 gctgaaatca ttattattac tccaccgtct ttacaagtag cttcagctgc tgtcattcct
     4021 ttaacagatt gataaatatt ttggtctagt ggatatccac catttgtagt tacaacaata
     4081 tcagatttta tagaatttac ttttgataac tctgttacaa atctacatcc tttagtatgt
     4141 gctttttcga catcaccagc aaaggaagca attatttttt tatctttatc tattacaaca
     4201 ttcaatatga atgctaattt tgcaacttta gctgcatata ccatatcctc atgaatagga
     4261 ttattattca attttccagt tcttgaatta tcactatcta taaagtcact acagtgattg
     4321 tacattatag tctttgcaga tgcgattcct ggaagtacgc tttttctgcc acctgaaaaa
     4381 cctgcaaaga aatgaggttc tataaaacct tctgctatca aaagttctgc ctcatatgct
     4441 agtttgttta tatataaatc tccacctgaa ggcaatatcc ctactttaca catagattta
     4501 tcatcagtag atatatgcat aactatatct tcattatcta ctatttcttc tcccatttta
     4561 taaattagct cttcccttgt acttggccta tgaaatccag ttgccactat aatcttcaca
     4621 tcaatttctg gattaacatt cctaatcctt cttaatataa ttggcatagt tattctactt
     4681 ggtacaggtc ttgtatggtc acttgtaatt atgactacat tctttttccc cttaaccagt
     4741 tcttccagag aattagagcc tataggatta ttcatagatt cttcaactat ctcttcttga
     4801 gtcttatgtt ctatataatt atccgatttt gactccaaaa caccgagaaa acgttcatca
     4861 ggtatttcta aaatcattcc ttgttttgaa tacggtactt ttaactttac catttttaat
     4921 ctcccattat aataaactat aaaattggta tgtggtcata ccagttttac atttatatga
     4981 tagtaccatt atctaccatt tgcaagaata atttatttta taacaaactt ttaattttca
     5041 ttttttctat aatttttact tataaaatcc atatgttcct ttgctttatc acatgcaagt
     5101 tctacatcat tttctacaac tgcattgtat atagcttcat gctgtttaag caatatattt
     5161 tcattaaaag gttctgcgat tattttttct cttgcatctt ctataaatcc atcaaataat
     5221 tttgatgctg tcataaaaag actttgaatt aaataatttt tacttaatgt tgctagttta
     5281 ctgtgaaatt tttggtctaa taacaccagt actcgattat tatctttttt attagtttct
     5341 accctcattt catctattat atgtttcaat tctataactt cagaagaagt tattctcttt
     5401 gaagcaagtt ttgcaatctc aatttctagg attattctaa gctctaatat atcttcaagt
     5461 gtaccattat taagtttaaa aatcatagat aaaggttcta ttaaagtttt ttctatattt
     5521 gtacatataa aatttccttc accctgtctg ctttctatca ctcccatagt ctctaatacc
     5581 ctaatagctt cccttatgga agttctactc acattcattt gttcagacag ttctctttct
     5641 gatagtaatt tatccccctt ttttaactct ccatttagta ttttatattg tatttgctca
     5701 attacttgtt cataaacttt tttactagct atactattaa acattttttc ctccaatatg
     5761 ataatcgttg ttgtaatata tatctattat atatcttaag ccattacatt catatatttt
     5821 tattactttt aatataatat cttataactc aaatttacag gtgatattat gaaaaaaaat
     5881 aaaattattg ctttttttgc tccatctatc atagttggag ttctagtttt agggattatc
     5941 ctatacccaa atgaagctat aaaatctgca agagaaggct tttctatatg gtctaatgtt
     6001 ttagttccat cactactacc atttataatt ggagcaaact taattgttga tttaaaaata
     6061 gtggatatta tagggtttat catcaatccc ataactagat ttgtatttaa tgtttcagga
     6121 aagagcgctt tagcttttgc tatatctaca gtatctggtt atccagtagg agtaagttta
     6181 gcttctgaat ttaggagtaa tggacaaatt tctaagtatg aagcacaaag acttgtgtct
     6241 ttttgttcta catcaggacc tttattcata ataggttcgg ttggtacagg tatgttccaa
     6301 aattcaaact taggttattt aatgattctc tgccattatc taggaactat attggttggt
     6361 cttttcttta gaaattatgg taatgaaaac cttccaaaga caaaattgga tttaaaaaca
     6421 aatataaaaa atgtaatcaa cattagaaat tcaagtggga atggtttttt tgttttattt
     6481 ggaaatgctg tatttaatgg agtaaataca ttacttacag ttggtggatt tgtaattgtt
     6541 ttttcagtag tatttaaaat actatctctt tttaatgtaa tctcattaat agcatcagtt
     6601 atatatttgc ctctttcatt tttaggtgtt agtaaagaac tctgtcaagc atttgtaagt
     6661 ggtctatttg aaattactat aggatgcaat aaagtatcta gtataacaag ttctccagaa
     6721 attttaagag catctttagc tagttttttg ataggattta gtggattatc catcctagct
     6781 caatgttgta catttctatc aaaaacggac atagatttaa aattatacat actaagtaaa
     6841 tttgcgcatg gagttttagc tgctatcttt atatttatac tttatccaat tgcaaactct
     6901 gctgtgttgg tttctagctt tgctgataca tacaacgtta tatataataa tttaatctgg
     6961 ttttattacc ttagctatta tgatacaatt attcaaattg tagtaataat ttatctaatc
     7021 tcagccgtat ttattgcaaa aaaacatatg aaaactttat ctacaaatgg gaaactaatg
     7081 cgatataaaa atactttttt cactaagaaa ttttcacgaa aaaaataaaa ctaataaaac
     7141 ttaactaaat acttccattt ttttattata tttgtcataa ttttaataac ctttttaatt
     7201 agtttaacag catagttatt tcatcacaat ttaataattt tattagaaat atttttcaaa
     7261 aaattctcct taatcacata tatcacactt gtataagttt actgggaaat ttttccatta
     7321 tataatattt catttatatt atataataaa taccgaccta aacttaccaa atcaaactaa
     7381 gaggagtggt tgaaaatggc aacaagacct atagaaatag acgaatataa aaagataatg
     7441 gaactattac atactggctt tacttatagt gaaaatggcg ttgaaaaaag atttagaaaa
     7501 aatcctaaag ttgcattggc actaatgtta gaagctaatt taggtctaag aatatcagat
     7561 attttaagat taaagatagg taattttaaa ggtaatatgt tggaaataaa tgaaaaaaag
     7621 acgggcaaat tacagtatag accagttaat aaaaatataa ttgagtccat aaatgaacat
     7681 gcaagaaagt ataatcttaa atcaaatgac tacttagtaa atataaagac aaaggctatt
     7741 caaaaacagt tgaggataat ttgtaaatac ttaaatctgt ataatataag tacacatagc
     7801 tttagaaagc tctatgctac tactcaattt gaaaagagta acaataattt agagttggtt
     7861 aaagaacttt taaatcacag ctctgtagcc actacacagc gatatataag ggtaactcag
     7921 caagctatta atgaagctag tgaaaatttc tttattggtt aattataacg tatacatttt
     7981 tctattatct tattaattca ccagatatta aaaaagagtg tctcaaaatg aactttttag
     8041 ttcatgagac actctttttt tataaaatca aatatatttt tattatttca acactggaaa
     8101 aaaggcttat ctctctattt tgagacaccc tattaaattt tcataattct tgcataaaca
     8161 tattaatcta tcttcaataa ttttttagct gattcactgt aattatttct ttttacaatt
     8221 tccaaaactc tatcaatctc atgttctgac tttgcactta caaaaatcgc caattcttca
     8281 tcatctataa tatcataact atctacatat ttatctaaac attctaaaat tccatcttta
     8341 ttattatacc caccaaatct aactactgca ctacactcat cttctataag tccacttaaa
     8401 tccaatttcc cttttgaaaa agactttata tttgaacttc tattatctat tatatctata
     8461 atatctgcat aaacagcact tcctgttgca cctcttccag ctcccttacc tacaaaagaa
     8521 agttcaccta ctgaatcacc tactaaaata actacgttga actcattatc tatctttgaa
     8581 agcatattat tattgtccac taatgctggt ctaacaaatc cacttacttc tcctgattca
     8641 tacttacttc tagcaaccaa ttttatctta caatttaatt tttttgcata ttctatatct
     8701 ttttcatcta ttgaagttat tccttctaat aataaatctt tccaatatac tatttttcca
     8761 taagataaag tagacaatat agataacttt ctagcactat catatcctaa aacatctgca
     8821 tctggattaa cttctgcaaa tcccaactct tgtgcctgtt ttaaagcgac atcatacggt
     8881 aaattttcat catacatctt tgataatata aaattacaag tcccatttaa aatagcgttt
     8941 atgctcttta tattgttgcc ttccagtgat tctatcaatg gttttaaaac tggaatacca
     9001 cctgccacag atgcttcaaa cttgatgcta acattattta agttagctaa ttctataagg
     9061 tcactacctt tttcagccag taaatcttta ttagctgtca ctacatgtat ttttttattt
     9121 aacgctcttg ttacataatc aaaagctgga tttaatcctc caattacttc aactagtata
     9181 tcaatatcct tgtcaaatat atcttctata tcagtagtta tcttatcaaa gtacttctta
     9241 tacttgtgtt tttctttatt tttaactaaa attccaacaa cttctatatc tctttttgaa
     9301 gtattatttt ctattaaatc aattaatcca cttcctactg ttccataccc taatattgct
     9361 atcttcactg taacataccc ccatcatttt gattatatta aattatttat atatataaat
     9421 ataccaataa aaaaagcttg agtataaact caagccatat gacgggttta tactcatctt
     9481 tcagcataaa tgctgcagga tttagcacca atgtataatt aaatatactg gttgccgggc
     9541 ttcacagggc cagtccctct gccactcttg ataagattgc taatttaaat ttatgtttaa
     9601 tagtataata caatttcttc caattgtcac tattttttta acttatttcc ctaaaaggat
     9661 ataaactatt atctaacagc tcttttagtt ggtttaccct tttttgtaac attattattt
     9721 ttagtctttg ctttcgcttg ttcatgtcct ggaaaaagta ttttaggctc ttcaatattc
     9781 ctcttgtaaa tagtaaattt aaaaccaata gcctgcacaa attcagctct taaagcttca
     9841 catactgcat tagcagtttc tttagtatca agacctgcat tttctaatat agtaactttt
     9901 actaattctc tagttcttaa cataccatct aattgctcta agaaactctc tgtaacacct
     9961 tctttaccaa tttgtgtagt aggctttaaa gtatttgcca aagatcttaa atacgccctt
    10021 tgttttcctt ttaacatatt ctcacctcta taattctata cttaaattat ctataatcta
    10081 tatttaaatt aattgtaaaa ctcaaattct aattcataaa ttttaaccac atctccatct
    10141 tctattccca tttctctaag tctatcaaaa acaccttgac tctccatagc tttttggaaa
    10201 tattgaaggg attccatatc atcaaaattt acagaataca ttattcttct aagtgcttta
    10261 cctgtaacta catacacacc atcttcaata tctatactta actcttcttc agtaccaata
    10321 tcaagttctg gtctatacat ttcttcttct gaaactagct ccacatcttc tacttctttt
    10381 agcattttag aaacataggc tattacttca tctatgcctt gacgagttgc tgctgacatt
    10441 ttaaatactg tatatcctct gccttctaat tcactcttaa acttctcaaa cttactctca
    10501 tcttctaata tatcaaattt atttgcaact acaacttgtg gtctctttga aagcttttca
    10561 ttgtacaatt taagctcatc atttatcttg tcaaaatctt caatagggtc tcttccctct
    10621 aatccagata tatctacaat atgtatcaat acctttgttc tctcaacatg tcttaagaac
    10681 tcatgaccta atcctatgcc ttcagaagca ccctctataa ttccaggtat atctgcaagt
    10741 acaaaactat ctccaaactt agtttgaaca acacctagat ttggtgtaag tgttgtaaaa
    10801 tgatagtttg caattttggg tttagcttta gttacaactg atagaaatgt tgatttacct
    10861 acatttggaa atcctaaaag accaacatct gctatcattt taagttcaag agttatccat
    10921 ctttcttctc catctgtacc agattttgca aaagctggag cttgtcttac tgcatttgca
    10981 aagtgttggt tacctttacc acctatacca ccttttgcaa ctatagctgt atctccttct
    11041 ttttttaaat ctgctaatac aagacctgta gcctcatctc ttattactgt acctgctggt
    11101 acttttaata ctaaattttc tccattttta ccagctcttt ttcctttgct tccgtcgcca
    11161 ccattttgag cttgatattt tttctggtac ttaaagtcca ttagagttct aagccccaaa
    11221 tcaacttcaa atatgatgct tgctccacga ccaccatctc caccgtctgg accaccagct
    11281 ggaacgtatt tttctcttct aaatgctacc gaaccatttc caccatttcc agccttaaca
    11341 aatattcttg ccttatctat aaacaaattc ttcacctact taattaaaaa cttttaatta
    11401 gcattaccaa aaataccatt ataaattcat cagtattata agaaaaagga gtgcctcaac
    11461 actcctttta tgtatattac tcagctattg ctactggata tatactaact ttttttctct
    11521 tcttgtcttt tctttcgaat ttaacagtac catcaactaa tgcaaataaa gtatcatcac
    11581 taccttttcc aacattagtt cctggatgta ttttagttcc tctttgtcta actattatac
    11641 ttccagcagt tactaattga ccatcgaatc tcttaacacc taatctttta gatatagaat
    11701 ctctaccatt tttagaagaa cctacccctt ttttagatgc taataattgt aaattcatgt
    11761 ttaacatatg gagtcacccc ctactttttt aaaattttta tattttttgg gtattcccgt
    11821 ttaatttctt taaccgctaa ttgaaagtga tttagtaata attgtgcgtc ttttacatgt
    11881 gtatctttga ctatacactc tatatgacct tcttctccta ctactttttc aaattcaatc
    11941 ttctgtaatt tgtctaaaga atttataact gcaatagcat tagatgtaac tgcagcacat
    12001 actatatcat agcctatttc agcaaaatca gcatgtccct ttaaacaaaa accttgcaac
    12061 aagaaatcat cattataata atatttaact tttatcataa cttaaccaat aactaagcgt
    12121 ttattttttc tacaactatc ttagtgtatg attgacgatg tccttgtttt cttctgtaat
    12181 ctttttttgc tttatacttg aaaactataa ctttcttagc tttaccttgt tcaacaactt
    12241 tagcttgaac agatgctcct tctactactg gagaacctaa ttttaattct ccatctttag
    12301 agcaagctaa tacttcgttt aaagtaacaa catcacctgc gttagcttct aatttttcaa
    12361 cgaataatac atcaccttca gaaactttat attgctttcc accagtcttt actatagcgt
    12421 acattaaata cacctcctcg gcctcaaact cgccagtaca aggtacttca tttgaagatt
    12481 taaaccttta ctgtgcggca ttacaattat taattataaa atatttacat tttattgtca
    12541 actattttat cgaaaataac attggtttta ttgtgcttta atgtactatt tgcgtttaaa
    12601 gaaatcttta tattatattt ttcaccaatt ttatcaatta tatccatata atattctttt
    12661 atttgttcaa aaataaaatc attaaattct accattatgt ttttgtaaac agtatgttca
    12721 cttattctca taatttcttt ttcaatacta tctagtatat aatgtacaga ttttattgtt
    12781 tgttcaccat cacaacatgg acaagacatt aaataatact tatcaatgga ttctttttct
    12841 ctacgcctag caacttctac taaaccaagt tttgtcatac ctaatacttc agcttttctt
    12901 ttatctttat taaattcttc ttctaggatg tgtaaaatct ccttttgatg cttcttttta
    12961 tgcatatcaa taaaatcgat tattattatc ccagctatgt ctctaattct aagctgtctt
    13021 acaatttctt ttgcagcttc aagattagtt ttataaacgg tttcatcaag tttaccagtt
    13081 ccaataaact tacccgtatt tacatctata accgtaagag cttctgtttt ttcaattatt
    13141 aaataacctc cactctttaa ccatactttt ttatttaata atttttctat tttagattct
    13201 attctgtata aatcaaatac atctctattt tcctcaagac acagtttatc aacataagtc
    13261 ttatctatac ctcttaaaat agacttaagt tcagagtact tatcataact attggtaatt
    13321 atagattcaa tatcatcatt cacattatct tttacatatt tagttgcaaa atctaaagac
    13381 ttatataata atttcggacc tataccaagt ttatattctt ttaaaatgtt ttcatacttt
    13441 aatttcaact cttctatatc tttctttatt tcatcatgag tgcaaccttg agcctcagtc
    13501 cttattatta atcctagatt ttccttattt actgccttag taattttttt taatctaaat
    13561 cgttcttttt catcagtaat tttatttgat attgtagttc tatcattgga aggtatatag
    13621 acaatatacc ttccagaaat actaatctca gtgtttaatt ttgcgccttt agttcctatt
    13681 tcttctttat ttatctgaac caaaatatcc tgaccagatt ttatagtact tccctttttt
    13741 aattgtaaat aagcaagttt cttaaatcca atatctacaa aacaagcttc tattcctggt
    13801 ataacttttt ttacgattcc acgatatata ttggatacag tcttcttatt taaattgtct
    13861 tcaacaaaaa gttctgttaa tctttcatct tcaagtactg ctgttttctg ggaccctatt
    13921 agggactcaa taactatctt tttcacaact tcacctctcg agcttacata ggagtcacta
    13981 attccccatc ttctaataca tataaatctc ttctcaatat atcaacatct aatgggtcaa
    14041 tatcaagttc aaacatctct aatatctttg gtataagtat attagtattt aaattagttt
    14101 ttgagcctgt agctatagta gcagtaagag taatatgttc atcttctaaa tttaaaacat
    14161 caaaggttct tatcataggt cttatatcta cttcaaccat cttacctttt ttattctttt
    14221 ttgttatgat tatttcttta ttattcataa agtcaattac ttttcttttt acaaactctt
    14281 tcgtcatagg tcttttcaaa tctatattaa acaaatattc tccatagtct atcacagaag
    14341 aaagagaagg tgtttgtcta tcaatttctt ttgctttgat aaaatctatc ccatctggaa
    14401 gttgaattga tactttattt aaaaattcct ctactgataa atcatcttct tctatttcaa
    14461 tatctacata ctcaccttga ctttctgtcc ctaaagccaa agcattgcca taactcattt
    14521 tagggtgagg attaaatcct tgagaatgtg ataagtttat ttcagctctt ctaaacgctc
    14581 tttgtaacaa tctctgaagg tcaagatgtg atatatatat catgtcgcct tcctttttaa
    14641 attttactct tattatctta ctcatattag caaagccccc ttcctatatc atgtatattt
    14701 attccacaac cattgcagtt atgtctacaa tcagaagtga ttttttcttt tttagcattt
    14761 tcgtcttctc taattaagaa ttttttacta atacctacat ctatatggtc ccaaggaaat
    14821 acctcttcat agtttctatt tctatgtgca taaaaatcta tagaaagatt attcttttcc
    14881 atagcttctt tccatatatc taaattaaaa tgttctgccc atccatcaaa tttagcacct
    14941 agtctaaatg catcatatat aacttttcct attcttctat cgcctctagc tactactgct
    15001 tccatcaaac tagtctttga atcatgataa ttgtatttta tattgttatt tcttaattta
    15061 tttactaaat gtctctgctt attaataact tcttctgtag tatcttgacc atgccattgg
    15121 aaaggtgtaa atggctttgg tacaaatgta gaagtactca ctgtaacact aaaacttctc
    15181 tttagctttc ctccatttac ttttctataa atatcaataa ctttataagc taatttagct
    15241 ataccatcta aatcttcata agtctcagtt ggtaaaccaa tcataaaata caacttaaca
    15301 ctattccacc ccatctcaaa agctctttct gttgcatttt ccaaatcttc ttctgtaaca
    15361 cctttattta taacatctct aagtctttga gttcctgctt ctggagcaaa agtaagtcca
    15421 gattttctta cttgttgtat tttatcagca atttccattg aaaaattatc taatctaagg
    15481 gatggaagcg aaatcccaat attattagat gcatattcat ctaccaaata atcagtcaac
    15541 tctgataact tactataatc actcgtacta agtgatgata atgatatttc atcatatcca
    15601 gtattcttaa ctaatttatc taatatttct ttaagtcttt taacactctt ttctcttatt
    15661 ggtctgtata tcatacctgc ttgacagaat ctacatcctc tagtacatcc tctaaataat
    15721 tctagtacta ttctatcatg aacagtatct atataaggta ctattagttt ttctggataa
    15781 tctacagtct caacatcttt tattatcctt ttagtaggat ttttagtaat cccttctcta
    15841 ttaggagtta cactttgaac tgtaccatct tcattgtact tgacatcata aaaacttggt
    15901 atgtatactc cttctatgct agatatttta tataaaaaat cttctctagt cgttttattt
    15961 ttcttccatt ctttatactc atttactact tctaaattaa cctcttcacc ttcacctaat
    16021 attactacat ctacaaaatc tgctaatggt tcggaattat aagcacaagg accaccaacc
    16081 attataaatg ggtcttccaa agttctttct tcttttaata ttggtatatt ggctaaatct
    16141 aatatattta agatattagt gtatgaaagt tcatattgaa gtgtaaaagt gacaaaatca
    16201 aagtttgtta taggctctct tgactctaat gcaaataatg gtatactttt ttctctcatt
    16261 atattttcca tatcaactgc aggagaatac actctctcac aaaatacatc ttcatcctta
    16321 tttattacat catataatat atgactccct aagtgactca taccaacttc atataaatct
    16381 ggaaagcaat gtgcatatct tatcaattca ctattactag tatctttatg aattgaattt
    16441 atttcatttc caagatatct agcaggcttt tctacttttt ttaagatttt tttcaagtct
    16501 actttattca tttattaaaa ttcctccaaa caagctcttt atttttattc ttatttataa
    16561 gtaatattat acacgataaa atattttcta tcaaaaatat aactaaaata ttgaatttaa
    16621 aatacatatt attcacaaaa tagaatagct aaaaatattt aagaatattt tctattaaat
    16681 ttttattgaa atacaatttt tttacaaagt atgatatatt ataaacataa aactattgtt
    16741 ttacatgact tatcatgaca tcttttgttt cattcacctc aaaatggggt atgttactta
    16801 tataatgata aattttagga gggttaaaaa tgagtaaagt ttttgaagta aaaaaagata
    16861 tctactttac tggcgtagta gatgaaggtt taaaagtatt cgacataata atggaaactg
    16921 agtttgggac aacttacaac tcttatttaa tcaaggacga aaaaacggtt ttatttgaca
    16981 ctgtaaaggc aaattttaaa gatgagtttt taagtaattt atcagaggtt actgatatag
    17041 ctaaaattga ctatgttgta attcatcaca cagaaccaga ccatgcaggt agtttaaaat
    17101 acttattaga tataaatcct aatatagaag tttattgtac taaagctgcc aaactatact
    17161 tagatggtca aataaataga ccttttaact gtcatgtaat aaaagatggt gaaattttaa
    17221 atatcggtaa aagaaatctt agatttataa ctgctccatt cttacattgg gttgatacaa
    17281 tgtttactta cattgaggag gacaagactc ttttaacttg tgatgcattt ggttgtcact
    17341 ttgcaagtgt tgatgcagag gtagtgaata gtgaagatta tctaaaatca gctaaacact
    17401 actatgattg tatagttaag ccatttgcta aacatgtttt aagtgctgta gataaggtgg
    17461 taggactaaa catagaattt gatactatat tgacttctca tggtccaatg cttactaaag
    17521 acccaatggc tgctgttaaa agatatgttg agtggtctac agaagctgtt aatacaacta
    17581 atcaaaatca agtttctata ttctacttat cagcttatag taatacttta gagatggcta
    17641 aaaagataaa agaaggactt gataaagaag gtgcaaaggc tgaattatat gatttagaag
    17701 atatgacatt aacagaaatg cacgatacat tagtagtgtc aaaagtaata ttactaggct
    17761 ctccaactat aaacaaaact atggttaaac ctatgtggga tttattctct gttatagacc
    17821 caatggctaa ccaaggcaag atagcaggag tatttggttc atttggttgg agtggtgaag
    17881 gtataactat ggctgagact ttacttaaat ctatgtcatt caaaatgcct gtagaatctc
    17941 ttaagaaaaa attcttccct agtgaagaga cattaaaaga gtgtatggca tttggtgcag
    18001 aatttgcaaa attagttaaa tagccattaa tcacacataa tattatttaa aataaatgat
    18061 tttatattta aaaataaatt aaaaaggagt tgtctcaaaa ttaatttttc taatctaatt
    18121 ttaagttcaa ctcctttttt attttctaaa atgactgata taatgataaa tgcacccttt
    18181 gtgcgagggt gcattattta taatatgttc tatttgtaca ctagtatatt tgtattaatt
    18241 cacatatcta gcgtatgcta ccatccctct tccagaatta gatgccgttt caccaatttc
    18301 cactttaata attacattat tttctttaca cttttttgat aattctgctg cagaaaggtt
    18361 cttaccagta aaatctaaag aaaatggctt atcatattca aaaggatact catcaggtac
    18421 accatctgga gctaatgcaa ccataccata tggtgtattc ataaacccta ttacagtaac
    18481 tgtatgcttt gatgggtcta aatttgaatt tttgcttgta aacttaattg tatttattga
    18541 tgagttgatt aaactacacg gaactccctc atacatttca atttttggac gtagattatt
    18601 tccataaact tcatcaagta ttgccccttg ttgatctgct atcatagtgt gattcccagg
    18661 tccaactggc gcagaaggct tagctggcgt aggtttctta acagttggcg ttggattctg
    18721 tgtctttact acttcgccaa cagctggata tgctttcttg tcaaatggta atgggtgagt
    18781 aagactcttg tatatgatgt ctgctcctgc tgatttgttg gcatcaatat ctacatgata
    18841 caaacgtcca tcctcaaaac gaataagatt ccactgatgg tcatttgttg tactatgtac
    18901 ataataacat tcaattccca tagcatcaca taaagctttc aagctcctac acattccaga
    18961 acaagcacct tttttagtaa ccaatgtata agcagtattg gctccctctg ctgcgttcca
    19021 gttaacatat gttgtgttgt ttttaatgtg attatgtata gatacaatcc actatggaga
    19081 aatataaaag aaaagagtat aacaaacaat aaaaaataag atgattcaaa ttttattttg
    19141 aaacatctta tttactctac aaattaagat tatttggtga attatttaat tattattttt
    19201 tattaaagaa attttaaatc ttaatataaa atacgctatt atacataata atccaaatgc
    19261 aaatgctact ggaattaaca tctccgattc aaaataaaag aaatattcta agaaaatatc
    19321 tggatttact atattcccat tcttgtctaa aaaatctttt ccattatatc ctaaatgctg
    19381 tataaaatta cttatgttat tataatctgc ttccctctca tataccatat tagcattcaa
    19441 tcctgaagaa cttaaattag tgtttccatc tttcatatct attacatata gtctaagagc
    19501 tttgacttta ctatacttat ctttatcaag tattttttca agttctaact tatattcacc
    19561 aatctttgtc atatcatgtc tttctgccaa ttgctttatt tctccatcaa gttcttttcc
    19621 taaattatac atattatgtt gtaaactatt attgtaatac cacataaatc cagaaattaa
    19681 aaatgcaatt atacttatgt aacctaatac ttttttaaac cccattacaa ttgatttatg
    19741 cctatccaat gataaagata gttcttttat tatattacat aattcatact gcatttcatc
    19801 accatcatca aatctttcaa ttgctttttt acatgcttct tcttcatcta atccttctaa
    19861 tttaaattca tttgcagatt ctataagatg gcatctcatt tcctgtttta attctatagt
    19921 acttttattg tcacattttt tatatagttt atctaaatat tcatctatta gtttcataaa
    19981 ttaatccacc tctcccaaaa attgattcat aaccttttta ataaatctcc attcttgttt
    20041 ctttatttct aaaaaatctt taccttcatt agttaaatta taatattttc ttcttccacc
    20101 tgaactttgt tcattgctat aataagattt aattaaattt ttagattcca ttcttttaag
    20161 tgcaagatac attgttcctt cttttaattc aaactcactt ctttctctta cttgctttgc
    20221 aatttcatat ccataacaat ctttcttttc taatatagaa acaattaata tatcgatata
    20281 accttttaac acttctttat ttaactgcat tatgtaccac cgccttttca cctactactc
    20341 tataatgtat agtacttata tttacta
//
LOCUS       DAENCV010000063        20149 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAENCV010000063 DAENCV010000000
VERSION     DAENCV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.064"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(28..939)
                     /locus_tag="KQD82_002339"
     CDS             complement(28..939)
                     /locus_tag="KQD82_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG4380807.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1909..2760
                     /locus_tag="KQD82_002340"
     CDS             1909..2760
                     /locus_tag="KQD82_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG4380808.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            2764..3234
                     /locus_tag="KQD82_002341"
     CDS             2764..3234
                     /locus_tag="KQD82_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380809.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            3247..4557
                     /locus_tag="KQD82_002342"
     CDS             3247..4557
                     /locus_tag="KQD82_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4380810.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            4769..5515
                     /locus_tag="KQD82_002343"
     CDS             4769..5515
                     /locus_tag="KQD82_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4380811.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     ECSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            5877..7418
                     /locus_tag="KQD82_002344"
     CDS             5877..7418
                     /locus_tag="KQD82_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG4380812.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            7453..8559
                     /locus_tag="KQD82_002345"
     CDS             7453..8559
                     /locus_tag="KQD82_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4380813.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(8937..10223)
                     /locus_tag="KQD82_002346"
     CDS             complement(8937..10223)
                     /locus_tag="KQD82_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4380814.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(10346..13444)
                     /locus_tag="KQD82_002347"
     CDS             complement(10346..13444)
                     /locus_tag="KQD82_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG4380815.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            14148..14300
                     /locus_tag="KQD82_002348"
     CDS             14148..14300
                     /locus_tag="KQD82_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380816.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(14592..15296)
                     /locus_tag="KQD82_002349"
     CDS             complement(14592..15296)
                     /locus_tag="KQD82_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380817.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(15287..16894)
                     /locus_tag="KQD82_002350"
     CDS             complement(15287..16894)
                     /locus_tag="KQD82_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG4380818.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(17148..18326)
                     /locus_tag="KQD82_002351"
     CDS             complement(17148..18326)
                     /locus_tag="KQD82_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380819.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(18552..19046)
                     /locus_tag="KQD82_002352"
     CDS             complement(18552..19046)
                     /locus_tag="KQD82_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG4380820.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(19047..19808)
                     /locus_tag="KQD82_002353"
     CDS             complement(19047..19808)
                     /locus_tag="KQD82_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG4380821.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         6818 a         3289 c         2465 g         7577 t
ORIGIN      
        1 taataaaatc tttaatctag cattatatta taatgaataa attttagaca actcccttag
       61 tctagttatt acattatccc taacatcttt aggttcaagt acttcaacat tgtcaccaaa
      121 cgataaaata tatccataaa tccattcacc aataggaaat tctgtaatta catcatatgt
      181 atcatcttca tttttagtta ttaaatcctt attaaaatca tcaaatactc taaaaagcat
      241 cttttcttta aacctcaatt tcaacgttat agtattttcc ttaattactt tagaatcatt
      301 caaacatact tcttctatta tctttcttga aaacacttca tcctttatag ataaattcct
      361 tatcctacta attctaaata tcctaaaatc atcttttagt ttacaaaatc catacaagta
      421 ccaagtctta ctcttataca taagcttcag tggttcgaca gttctgtttg tctggcttga
      481 caaactattt acataattaa aatttatagt attacgcctt aaaatagctt cttttatttt
      541 tgtaaattta tcatcttcat taaaattact tccccattca gtaaagtcta tttcaatcca
      601 gtttgaaaaa ttctgctctc caaatattga gccaatttta tttactactg aatttatttc
      661 tggatactta gtcgcctgta atgttttcag tgcaactatt aaactctctt tatctttttt
      721 tgacaatata gccttgtcta tagaatattc ttctattaaa gatatccctc ctcctttgcc
      781 tttcgtcata tatactggta ctcccgacat agacagagtt tctatatctc tatatatggt
      841 tcttgtagaa acttcaaacc tatctgccaa ctctttagct gttactaatt ttttatttaa
      901 tagaattact attatttctg ttaatctatt tattttcatt cattaccacc tactaaaatt
      961 attaaattta tttaatctaa attaatcata ctaattttta aataaacaaa atcttagaat
     1021 cttatctaaa ttattcttac atgattttat ttactttcta ttatctttct cactttggat
     1081 attatcatct taatttattt attactatat caatttactt attatcatct catttgttat
     1141 cgtcttactt aatttactta ttgctatatc aattccactt attatcatct tatttaactt
     1201 acttatttct atatcaattc acttattatt acctcacttg ttatcatctt acttaattta
     1261 cttatttcta tatcaattta cttattatca tctcatttgt tatcgtctta cttaatttac
     1321 ttattgctat gtcaattcat ttattattat ctcacttgtt atcgtcttac ttaatttact
     1381 tatttctata tcaattcact tattatcatc ttaattcatt tgattattat catttacttt
     1441 attcaatttc tatatcaaaa attagtattt gtacttaata cttaaaatta atttaaagtc
     1501 tttacatatt atttattttt tattgtaata ttcatttcca tactttacta agctacttcc
     1561 actcatttat tttaaaattt caatttacta tttctcaatt ctatattatt atttttcaat
     1621 tttatattac taattttaaa gctttttagc tattattcct acaatattta aacaattttt
     1681 tcataataaa gctatattac aatatattca aaatatgtaa tattttagat atttttcgtt
     1741 gacaatgtta aatttttgtt ttatccttaa ttaaggacat gttattgaca tttctgaaac
     1801 ttatttgaaa tagttttttc attttattta ggaaaacgta ttatacttaa gaaaacgtat
     1861 tttctcattt cagataagta aaatatttat tcttttgagg aggaaatcat ggaaattaat
     1921 aaaaaacatt ttattcaagt atttttaact tgcttaatct tggtagtaat atgtgaattt
     1981 atagggcaaa gaactatagc tattggaaaa gcctctatag tactgttccc tatgctttat
     2041 gcagttataa ttggacttat aataacacca gatttattag gtaaaaaaat taaagcgctt
     2101 aaaaaagtgg ttggagaaaa agaaataaat attgctggtg aagttgttgg aattgcacta
     2161 gtaatgcttg gtataaaata tggtactaca gttggaccaa acattgacaa aataattcaa
     2221 gctggaccag cgtttatcgc tcaagaattt ggtcatatac ttgcaccaat cgttgcatta
     2281 cctcttgcat taatgtttgg aatgaaaaga gaagctgttg gagctactgc aagtataagt
     2341 cgtgaacctt ctttaggagt tatatcagaa aaatatggga taaactcacc agaaggtagt
     2401 ggtgtacttg gtacatattt aatgggaaca gttcttggta ctatatactt tagtgtacta
     2461 ggttcaatat ctatttatag tggtcttcac ccatatgctt tgggaatggc ctgtggtgtt
     2521 ggtagtggaa gtatgatgac agcagctgct ggttcattat ctaccatggt accacctgaa
     2581 atggcagata caatacttgc ctatgctgca acaagcaata tgatgtctag tataacagga
     2641 attatattct tagtttttgt gtccttacca tttactaatt ttatgtataa gattttagaa
     2701 ccaaaatttt cgagaaataa aaaaactaaa gaaattaaat ctacagaagg agagtgctaa
     2761 taaatgtttg ctaatttaaa tttaaagaaa agttcatatc aattattggt tatgtctgtt
     2821 attggagtta taattcttat aggacaaaga attagcgttg gtacatctat agttacagct
     2881 cttcctggta tggtgatgct aatattagct gctatggcag ctatgattat aaaagattta
     2941 tttcctaaat caatctttcc agcttttgga tttgcaacta taatcggcct tttacttagt
     3001 atgccatata gtccaacatc tgaagtattc ttaactaaca ctaataatat taactttatg
     3061 gctataacta ctccactact agcttttgca ggaatttctg ttggaaacaa aatagaagct
     3121 ttaaaagaaa tgtcatggaa aatagttata atttcactta ttgtatttac aactatattc
     3181 tttgcatgtg cgagtatagc tcatatagtt ttaagtattc aaggtaaaat ataggaggaa
     3241 tttattatga ataaagaaca attaaaatct ctagtaataa aaactataga tgaaaataga
     3301 gaaaaaatat taaaaatagg tcaaggaata tacaaaaatc cagaatatgg atataaagaa
     3361 tttaaaacta cagaagctgt ttcaaacttt tttgagcaag aattaaattt atctgtagaa
     3421 aaaaatatag cagttacagg atgcaaagca gatgcaaatt ctagcaagaa aggtcctcat
     3481 atatctatac ttggagaatt ggatggtatc tcatgtaaag aacataaaga tgctaatgaa
     3541 ataggtgctt ctcatacttg tggacataat attcagatag ctggaatgtt aggtgctgct
     3601 gttggtttag taaaaagtgg cgttttagaa catttagatg gtaaagtatc ttttatggct
     3661 actcctgctg aagagtttat tgagttggaa tacagagagc aattaaaaaa agaaggaaaa
     3721 ataacttatt ttggtggaaa acaagaatta ataaaaagag gttattttga tgatatagac
     3781 atatctatga tgtttcatgc ttctgattta ggagaagata aggctctagt tggtcctgag
     3841 agtaatggat ttgttggaaa aaaggttaaa tttataggta aagaatctca tgctggttct
     3901 gctccacatg atggaataaa tgctttaaat gcagcaatgc ttgctataaa caacgtgaat
     3961 gcacttagag aaacttttaa agagtctgaa agagttagat tccaccctat catcacaaaa
     4021 ggtggagata tagtaaatgt tgttcctgct gatgttcata tggaatcata tgttagagct
     4081 agaactataa atggtatgat tgatgctaat gaaagaataa ataaggcttt gatggctggt
     4141 ggaatggcag ttggagctga tgttgagata actgaaattc ctggatatct tccaatatta
     4201 cgatacaaaa gtcttgataa tttatttaaa aacaatcttg aagatttagg tctaaaagga
     4261 aaagttattg atggtggaga ttttacaggc tcttttgatt ttggagatgt gtctcatata
     4321 atgcctactc ttcaccctat gattggtggt gtaaaaggag ctcttcatac aagagatttt
     4381 gaaatagttg atgaagattt agcttacata attcctgcta aagcaatggc acttacagtt
     4441 gtagaccttc tttttgataa tgctaaggat gctaaagata tattagatac ctttactcca
     4501 gttatgacta aagaagaata tctaacattc ttagaaaaac acgataaaac tttataagtc
     4561 tatttttata atagggtggt atctactgta aaagttatac cactctttgt ttttgtaaca
     4621 gtatacttct tttcttttag tttttagatt aaactaaaat atattatcct ccttaaaact
     4681 tttagagcta cgttgaaata tcagtagaac tatgctatat tctttctaat taatataaat
     4741 ttctattcta attcaaggtg gtgaaaaaat gtttaatgca ggaaatcgaa ttataaatac
     4801 atggttgtac cctattcaag atggctttgt cttgattgat actggatatg aaaatggttt
     4861 taaacatttc aaaaaacgaa tttctaaatt caacataaaa atcaaagata ttcgatacat
     4921 atttctaact catgctcatg atgaccatgt tggttttcta aatcagattc tagacgaatc
     4981 tcctaataca aagattgtta tgcatgataa agctttagaa attctatata aaggtcaaaa
     5041 ctcttttatt ggtggttgta ccagccgtac tgcctttctc ttttgccaaa ttatgaaact
     5101 atttggaaaa ggtgagcata gtttttctcc acttaaacaa gaatttgaaa aaaggtgtat
     5161 tcttgtgtct gacaagaatc gtaaagaaat tgaaaaagaa ctaaatggaa aaattataga
     5221 tacaccagga catactgctg attcaatttc actactaatt gatgaaggtg ttttattttg
     5281 tggagattct gcaatgaatg gatttccaag tactcacaaa attaccatat ttgcagaaag
     5341 caaactagaa tttattcaat cttggaaaac aatcatcaat acaaaaccta aaatgattta
     5401 tcctggacat ggaaaaccat ttgaatgcag taaattagaa caaaatctaa tctatgtaaa
     5461 taaaataaat ttatatcctc tacataattc aaaagatatt cacagatttt tgtagaaaat
     5521 acaaagtaaa tttctttgat taattaaaaa atctaacact ttactacgct acttatttta
     5581 ataacttaat cttccactag cttttcataa aattttgttt taaaattaat tttaaagtgc
     5641 atatgttatt taaatacatt agataaaatt cgtcattaat ctacttaaag catatttaac
     5701 taacaccact caaccagaaa attcagaaaa ctgcattgac acaggaaaaa gtttatttta
     5761 tacttttttt aggacatatt gtggacacgt cttaaatcga caagcagaat ttatattgta
     5821 agattaacca caatacttat tttttaatta tatttttaaa cattagggag gatattatga
     5881 atgataacgt agttaagaag aaaaactttt ttgacaggac attaaacaga atagaaactg
     5941 ttggtaataa actaccagac ccagttacaa tatttttagg gttatgtgta cttttactta
     6001 tactatctag tctagttggt tctatgggta tatcagtagt tcatcctggt actggagaaa
     6061 ctataaccgc tgtaaattta ttgactgtag aacagcttca aattctttta ggaaacatag
     6121 tcagcaattt ccaagggttt gctcctttgg gtctagttct agtaactatg ataggtgctg
     6181 gtgtatgtga caaaacagga cttatgacag caacaatcaa agcatcagta agcaagattc
     6241 ctgaaacaag agtaactctt gttgtaatga ctataggtat gcttgccaat atagcttcag
     6301 atgcaggtac aatattattt ccaccattag ctgcacttgt atatcttggt gttggtagac
     6361 atccacttat aggtttattt tctggatatg cagcagtctg tctaggattt gccgcaaaca
     6421 ttatgataag tgtcaatgat atacttgctg caagttttac agttccagca gctcaaatga
     6481 ttgattctgg atttcaagcc aatgcaacaa tgaacttatt ttttatgata gcatcaactt
     6541 ttgttctaat acttttggca acatgggtaa cagaaaaaat tgtagctcct agatttggta
     6601 aatatgaagg aaatgcagaa ctagatgttg atggaagtct tagtgatgta gaaaagaaag
     6661 ggcttaaaaa agctggtata agtatattaa tatatgtggc tattatagta gctcttagtg
     6721 taatcggtaa aaaaccattc ttagctgacc cagaaacagg agatttacta tcaaacaatg
     6781 ctcctcttat gaaaggtatg gtacctataa tagcactagc atttttcata cctggtgttg
     6841 tatacggaaa aactataggt aaaataaaga aggataagga tgtagtctct ataatggcta
     6901 gtgcaatgag tgatatgggt ggatatataa tactagcatt tgctgcttca caatttttgc
     6961 aattattcac aaatagtaat ttaggtctga tactagctgt aaagggcgga gaatttttaa
     7021 aatcagcagg aataaatgga cctttattgt tgataggatt tattatatta tcatgtttta
     7081 taaatttatt tataggtagt gcatctgcaa agtgggctat tcttgcacct atatttgtac
     7141 caatgtttat gatggttgga tacaatccag cacttactca gatggcttat cgtataggtg
     7201 attgtataac aaatccactt agcccattat ttccgtactt ccctattata ctagcattca
     7261 caagaaaata cgataaagat gctggtatgg gtactgtaat tgctaatatg ataccatact
     7321 ctatatcatt cttaatagtg tggactatac tactaatatt gtttatggta tttaatattc
     7381 cactaggacc tggaatactt ccttcatata gcatgtaatt tatttaaatc tttctaaaag
     7441 gagaaataat ttatgttaga taaaaataga cttataaata attttatgga tatggttaga
     7501 attgatagcc catctaatca agaattagaa atgtctaagt ggttggtaaa ctacttaaaa
     7561 gaaagaaata tagacgccat tatagatgat gcaggtgaaa aatatggtgg caatacaggt
     7621 aatgtcattg cctatataaa aggtgaagaa ggaagtagac cactttgttt atgtgcacac
     7681 atggaccaag ttcaaccttg tttaggtgta aaaccaatac tagatggtaa tgtagttaga
     7741 agtgatggaa ctactacact tggtgcagat gataaagctg gaatagctgc tatacttgaa
     7801 gctttagaac atgttattac agaaaaaata ccacatagag atatttatct ttgttttaca
     7861 atctgtgagg aagctggtat gcatggtgtc aaaaacttta atcctgacaa cttgccttgt
     7921 aaagatatgg tgattttaga ttctggtgga gctattggtt cgatagcata caaagctcct
     7981 gctcaacaat ctataaaaat ctcttttcat ggaaagaaag cacatgctgg tatagaacct
     8041 gaaaaagggt taaatgctat attagtagcc tctcatgcaa taagcaacat gcacataggc
     8101 agaatagatt cattgactac ttcaaatata ggtaaaatag aaggtggtgg agctacaaac
     8161 atagtaactg ataaggttac attgactgct gaaattaggt ctcatatacc tgaaacttta
     8221 gagtatgagc ttaatcatat ggaaaaatgt tgtaaggatg ctgcatctaa atttaatact
     8281 acttatactt ttgaacataa tatgtcttac cctagttttg aacttagtag agatagtcac
     8341 gttttcaagt taagtgaaga agcaataaga caagttggta ttgttccaaa tccaatggta
     8401 attggtggtg gaagtgacgc aaatatatta gctaatcttg gatataattg tgctatatta
     8461 agtctaggta tgtatgatgt acacacagtt aatgaatatg taaatataga cgaattatat
     8521 gatactgcca aaatagttta tcatatgatt aaattataaa tatcataata gaactcttta
     8581 aattataaaa ataaaaacct atgtatcttc aactttataa gttatatatt agagttgttt
     8641 tacataggtt ttagtatttt atataataat ttgttttaac atttttcata ataagctttg
     8701 atattttata tagtaatttg ttctaacatt tttcataata agcttcaata ttttgcataa
     8761 taatttattc tagcattttt aataataggc tctagtattt tatattaata atttattcta
     8821 gtattttaca taattatttg ttctaatttt tttcataata agctttaata ctttatatag
     8881 taatttgttg tagaatttta tacaatataa tgatttatac agttttataa aatatcttaa
     8941 aacagtattt cagtaatatt ttttggtctt cctccaccaa tactagtctc ttttgcacat
     9001 attcgaccta aacctgcatt tactatttta tttaatatac gtcttgcact tctttcactt
     9061 acatttagat actctgctaa ctcttttgaa tcataaatct tactttttct attctcattt
     9121 atagcaataa tttttgcaac tgactcacaa ctcattccag tctttttaga tatttcgatt
     9181 accttttcat cagatagcat caaagaataa tttaattcat tttcggaagc taaaggtcct
     9241 cttatgtttt catcttcatc taccaaatat ataccaattt cttttgaatc tatggagtgt
     9301 cttaatgctt tatgtgcatt attttcagcc tgatacgcag ttactccagt accaattcct
     9361 atactaagag aaaagcccat gtcttttatc tctctcttta gattgaatag cttatcataa
     9421 ttatactcat tatcagctac ccctttatga acaaatacaa cataatcatt tcttccaagt
     9481 aaaaataagg aaccttgaat gcttcttaca tagtttacta caagcttatc catatctgat
     9541 ttttttatca tgtcagaata atagttatct atcttctctt tataatctat taaactcaat
     9601 atttcaacag caatttgaga aaatcttgcc ttacttagag catattcact ctttaccttg
     9661 tcataacttt ctttgattaa ttgtatagta gcttgtaaac gaaatacagg atagcctctt
     9721 tcttttaatt cattatatac tgcaccaaaa ccagttaaca ttatatccgt cttattttct
     9781 tcaaataatt ttatatgcca ttctacaagt tcagactctc ctacttccaa agaaaatgga
     9841 ttacaaaata cactcgttgc tttaatatca aattctttaa gtgcatcttc cacaacatat
     9901 ctatcaacta catctatact gaatttatct aattttatat ttgcactttt tattgcccaa
     9961 aatgccttca ttatactcgt cccacttctc ggtacaaatg aatgtggctt acttatctca
    10021 tacttagatt ttataaattc ataaactgca catcctgtaa atataatagc atcacattcg
    10081 tcttcacatt tagaaataac ttctattgta tctactactt tttctcttac atatatttta
    10141 gtctttaaat cactgtctat ctgttttaaa tcatccttta tcttaaaccc agagtctgaa
    10201 ggacctataa tacccactat cataaacttc gctcctaatc cataaatatt atacttttta
    10261 aaaattatat cctcactatt ttctacttct atttaattat tttgaactac tccatcctat
    10321 ctgcatttag aatggaataa ttagcttatt tttgataaag taacttctca aaatcagcac
    10381 ttggaatagg cttacaataa taataacctt gaattaccac acaatctaaa ctcactaaaa
    10441 attcagcttg ttcttttgtc tctacacctt ctgccactac atccatatct aaatcttttg
    10501 ccatggctat agtgtgcttt aaaactattt tttctctgtc tgtgattcct tcctcattaa
    10561 agaaaccaca atctatcttt aatgtatcaa tattaatatt ttttaacata tttaatgaag
    10621 aatatgctga accaaagtca tccatattta aaacaatacc atccattcta aggtcggcta
    10681 ttatcttagt aaataatttt atatttttaa taaataaact ttctgtaagc tctaattcta
    10741 aaaaagattt tggtatacta tatttattta ttaaacttaa aacaatttct ttaaaatgtg
    10801 gattatgcaa atgtaatctt gatacattta cagagatagg aactggctct tcacctaaat
    10861 ctatccattt tctaatcaaa gaacatactt gttcccatac atacatatct aatctgataa
    10921 taaacccatt cttctcaaat agtgaaataa atttaattgg aggtattata cctttttctg
    10981 gatgaaccca tctaaccaat gcttctgcac caacaatttt atttgcatta agattatact
    11041 taggttgtaa atatacatgg aattgcccat tttcaagtgc gtattccatt tccctttcca
    11101 tctgtttttc ttcaagtgca tcatttctta tagtatcatc ataaaatata tagttattga
    11161 tactttgttt ctttactttt ttgtgagcat atcctgccat ttcacacgca tttgtaattg
    11221 gaatatgatt atccaaaatt ctataaaccc caaaacatgg tactaacata tattcaaatt
    11281 tttttctatt taagctattt ttaatcttat aaattacctt atgaatatct ctatcagagt
    11341 aactctcata aaaaatgcaa aatacatctg catacatacg acaacatata ccattagaac
    11401 caatgttttt ttgaatcaac tctgcaacat atattaaagc tttatctcct cctctaactc
    11461 cataaatgtc attaattacc ttgaacttat ctatatccat cattataatt gcataacgct
    11521 tgtcaggata tcttttaagc attaaactta tttgttcaca aaaactttca taattgtata
    11581 gtcctgttaa tgtatctttt tggtttttat gtagttgcat aagataatct atatttttta
    11641 tagcaactaa aatatgtgtt tgtttctcac ctaacttatt tttactacta atctttaaca
    11701 tataaattaa tgcccaatag tactttttat ctattcctaa acgtctatac tcaaaattta
    11761 gttctaaatc gtcttgcttg ctattattaa gactatcaag agattttaga aggtggttat
    11821 cattaaataa tattatatca tcaggatgaa ttaaatattt ttttatgtat gaaaaagttt
    11881 tttcaagatt ttcctttata ggaaaactaa aaatattgct ctctgattga taaattggag
    11941 taattaaatt cgtatcaatg caaatagata aaattatatc atatgtatta taagctaatt
    12001 tttcataagc tttagaatac ccttttatca ttaaatcatc aaaataaacc ttatcaacta
    12061 actctttatg tttaaataaa atattattcg tcgaatcagc taagtcacta attactttca
    12121 tttcattatt tctaagtttg ttcttattat aatacctctt cttatcttgg tacattctct
    12181 catcagcaac tttaattagc ttatgtatat caatattatc gtcactccaa atactgccca
    12241 ctgctacact atatatcatc tcatccttaa attcttcact taaccaactc actaattctt
    12301 caaatagttg ttttgatata tcttcacata atacaacaaa ctcatctcct ccaatacggt
    12361 aaacattata agaatgaaag tattttttta ttattacaca tgctgacaca atagcagaat
    12421 cgccattagc atgtccaaag ttatcattaa tatcttttaa cccatttata tctacaaata
    12481 caactcctat ggatttaact tttccttttt gaaaattatc tatatactta atatatttat
    12541 ttctattata tgcaccagtc aagaagtcat agtagctcat ataatttaat ttattttcta
    12601 cttttctttt tttcatctca gttaataaaa aataagatag ggatgtcaaa agagaggtat
    12661 ctccaacagc tttatgtgga ttatcaactc caataaaccc tactacttta tcatcttgtt
    12721 ttagtgaaga tgtcattaaa ctttcaattt tttgaggttt taaaatttca tattctattg
    12781 gctgttcttc ttttatattt tctatatcat caattattac aaaacctttt tgttcgaata
    12841 ataaaaacca attctttaat tcattaatat caagatttgt tagtgcctgt ttttgttcat
    12901 caacaccttc attgcaccat tcatatgtat tagttacttt tgttctatct tctgatactt
    12961 caaaaacata tgctctatca gctttatgaa atttaccaat attggttaat acaagattta
    13021 cagcatcaga aaaattagat tctgttataa gatgtttaat acatccaact aataactcct
    13081 ttatctctaa tttttctgct attgacttac ttatatcttc cttttctgta atatcaagag
    13141 ctatttctaa tcgtgcatgt ttgccctccc atttaattaa cttgtccttt aatataaaat
    13201 gtctatttgt tcgagtattc gtattttccc atatgtaata ttcatcttct tttaaaatag
    13261 agtttgtaca aaattcgcag ggagaatctt tcccctgtaa tgctttataa cattttaaat
    13321 gggatatatc attaagtccc attgcctttt tcccaatttc attaatatat aaaagttcat
    13381 atgtatctaa agaagaaaca tatacaattt catccaactc atctattaaa tttattacat
    13441 ccatttatat atatctcctt ttattcattt tccagattga atttttaaag cttttaaaat
    13501 tttgctatat atttggcaat gatattatac ttctttatgt tttaaaatag taccttaatg
    13561 aggttctatt ttttagtaaa tagaattatt attgtgtcaa tcgtacaata gagttttgta
    13621 atacttaatt taatatattt ataattaact atcttattat gtatttacaa ttaattctca
    13681 tataaatgac aaatctccac ctcaattaag tattccaatc atatatgagg ataatacttc
    13741 attcatttaa aaatataaaa ctattatata ttaaaatttc ttcaataata aattatttag
    13801 attgttactt tgatatatta tataatatca caaaggctga tataaaaaga caaaaagggc
    13861 aattttttta tatcaaaaaa aatgttaaat tgatattatc catatagatg ctatatttaa
    13921 caattctact tacttccctt acacttatta gcatatagtt attaagagtt tgttgcaaaa
    13981 caacacttag cacactttaa aaagtgtggt ctaataagta atcaacgaag aagacataat
    14041 aaagcataaa atatgatata cttttccacg tcatcaaaac tgaggatttt attattaata
    14101 tatgataaaa tatgcttaat aaattaaatt ttaatgggag aatagttatg aaaatattta
    14161 gtttcttgtt gtttgtagtg ggaatatgtt tttgtattgc tggatttatg gggcattcct
    14221 cagctttttc tggtgcaaca ttgagttttt ttgcaactac aatgatagct gtaagacaag
    14281 tcaaaactaa gaaaaaatga aataggcaaa tcctaacttt acaagttaat attacataag
    14341 taattagtct aaaattagat actttttcta ttacagaaat acatcatcca ctttgtattc
    14401 tcttaaaaaa ataaaatagc tatgtaaatt caagattaga tagactaact ctctatccat
    14461 ttcattgtaa aatatacata gctatttgta ttcgatttaa aatacttaga ttttcttaca
    14521 taacaaataa tttctatctg attattactc atattcattt agtaatcact cagcaactaa
    14581 tcatttagta tctattcatc cgccatacgg taacctaccc caacctcagt caaaatatat
    14641 tctggctgag caggattctt ttcaattttc cttcttatac ttgccataaa aactctaagc
    14701 gactgattct cactttcata gtaatttccc caaactttat ctattataaa cttatgggtt
    14761 aagactcttc ctgaatactt agacatcaac tcaataatct tatattcgat tggagttaaa
    14821 tgtatttctt caccattaat tgacacaata tgattttcat aatctatctt taggttttta
    14881 acttcaaaag actttggagc atcattattt atattatcct gttgagcttt atttctaaga
    14941 gccacacgta ctctagccaa aagctctgtt acactaaaag gtttagttaa ataatcgtct
    15001 gctcctccat ctaaagcttc caccttatcc ctctcatgct ctctcgctga aacaacaatt
    15061 attttattag tctttgcaca tgccctaact cgtcctatta cctcaatccc atcaatatct
    15121 ggtagtccta aatccaaaat tatcaaatct gggtcaagag aatgtataag tgatatagct
    15181 gttcctccat tatctgtctc tacacaccta taattttgag tttcaagaga tacttttata
    15241 aatttacaaa ttggcttatc atcttctaca attagaacta atggctttat ttccacttgt
    15301 ctttcccctc ctcatcatac ataggtatgc taaatctaaa tgtagctcct ccagaaggat
    15361 tattaaaggc gacaatttct ccaccatgag cttctactat agatttacat atctccaatc
    15421 caagaccaat acctcttctt tcaagatttc tagacttatt cttagtataa aacctattaa
    15481 aaatattccc gatatcttca tttggtattc cttttccatt atctgcaact tcaaatagta
    15541 cttgatttga ctcttttttt acagttagtt ctatcttaga atttttaggt gtatgcctta
    15601 tagcattatc aattagattt actaacactt gttctattaa taatacatcc acatgaacga
    15661 ttattatctc atctggaata tttgttttta tgtctctact attggcaaac tttttgactc
    15721 ttaatataga ttcagctatt atttcatcaa caacctctgg tcgtttttca atatccaatt
    15781 ttccttcatc tattctggtc atactaagta tattctcaac agaatgagtt agccaactcg
    15841 tatcttcata tatatttttc aataattcta ctctagtttc tttatctatc acatcatcat
    15901 tttcaagtat tgttgaagat gaacccaata tacttgtaag tggagttctt aaatcatgag
    15961 aaattgacct aagcaagttt cctcttaatc tctctctctc tgcctcaaga tttgcttttt
    16021 tatttttctc aaataaattt tctctatcaa ttgccaacgc aacctgtgtt gaaactgctt
    16081 ctaatagtat cttttcattt tctgtaataa tatctatctt aaaacacgca acacctataa
    16141 caccaagaac atttgattct tgacctttta tagggaaata atatgcactg ctgtttttac
    16201 aatattttgt cccaattcca actggattac ctgtcctaaa agcttctttt atagcttcct
    16261 tttctaatac tgaatggaat atattctcac ttccatcatt gttacaaata taaatacttg
    16321 gcttacctag attattattc tcatctgcaa tagtcactat tacagttcta tttaatatat
    16381 ttactaaatt agacccacaa aattcaataa tttgattttt attttttgct ttaagcaacg
    16441 ttttgttatt ctcatagagc aatcttatca ttttttctct cttatgagaa agtctcactt
    16501 ctctttttat cctagaagtt aaagtacttg ttataattgc tgccattagc ataatacaaa
    16561 aggtcatagg ataatctttt ctataagcta aaaaagtata taatggatgc gtaaagaaaa
    16621 aattaaatga tagcactcct attactgaag aaagtattcc aaaaaaatat ccataggtta
    16681 tacttgtagc aaatagtaca cctaatatgt atactagtat aatattagat tcattaaaac
    16741 ctaccttttc aaaaactact gatattccag tagcaattgt cataaccaat atagttttta
    16801 tgaagtcctt aataattatc tttttactga gatagccatc ttcatcataa tcatactctt
    16861 ttaagtcttc atgttgtagt cgttcctttt tcatttacta caattcctcc ttattgttac
    16921 tgtttaaatt ggctctattt tatataatac aattttatca taataaattc aatcaataca
    16981 tttaattaat gtacttaaat tactaaaagg tgctgtctta aaatgaaaat aaatctctat
    17041 ttcaaaacag cacctatata tttaaattac taatttagtt tttacatact taaatcaata
    17101 gttttattca atttcataaa ttataatact tcaattataa aacatttcta tccaacaagc
    17161 ataaatataa agtgggcaca tatacctgca cataaaccac ctatagtatg cgaaagtatc
    17221 gctttaccag ccaatggtct tgcatctaaa gcatccatca tacctatgtg tgtacttaaa
    17281 tatccactcc aacacatacc cattgctgtg aatactgcta tatcattagg agttatagcg
    17341 cctgatttta taaactctgg tactaaagat attgctgctc caacagctcc taatgcagtt
    17401 acagggaatg ctattgcctc tggtgatgtg aatccaaata atggttctat tatgaagcta
    17461 atcttatcac caatagcagg taataatttt ataccttcat atgcagctcc tgtgtaaaca
    17521 gcttgacctg tagcagggtc tgtagatggt ccaaaagtta ataacataac taatgtacaa
    17581 actactaaaa ctcctggtat tatagccata cccatttcaa cacctaactt accaccttca
    17641 agtatagcat ccaatgttct ttggaataca ttaccatctc ttatttctct aaactcttca
    17701 agttttgcac ctttatcatt tatttgcttt tcagtatcat ttttagggtc ataattataa
    17761 tactttttag taaaagtcaa cataattcta acactaataa tactacctat tattgccgca
    17821 acattaccta ttatagctgg tagtacatat tctttacctt gagctatcat aaaagttgtt
    17881 acaataagtc ccataccaaa ggctgttcct aaattacata aagcaggtac ttgatacttc
    17941 ttaaaatatt gtgtaaatgt tttgtcttta gcaaatggta taatagctgg attatcagat
    18001 aaatatgttg caactactcc tgctatacta gcaccaggta agccccatat aggtctcata
    18061 agacccttaa atatcttatt aactaaagat attactccaa actctgaaag caatgcacta
    18121 agtgcacctg caagaactgc catagccatt attaaaaata ctgtgtctaa taaaagcgca
    18181 tgtgctgtac tcattattac tttaaacatc attcctgcac ccatgatgct tcctacatat
    18241 ccaaatccta ccgccaatag tacaataaat acgaaggttt ccatacttac tgccttgact
    18301 cttctcttat ccgacagctt ttccatcttg aaatcttccc ccttttaatt ttaaattaag
    18361 gttcctaaca ttggtacaaa ttttttggta caaactgagt tctaaaactt acactcgtaa
    18421 tgcaagttgt cagttttttg aaataaaaaa aaattacgtt tatagaataa gaatatcctc
    18481 tttaatttgg atactcttat tctattttat gttattatat tatcatcctt ttgtttaaaa
    18541 gtcaattttt tctatttttg ttgtttaatt ttatttatac tctttttata tttttcattt
    18601 tcttttatct ttgtaagtac tccatataag ttgacttttc taaatacagt agttataaat
    18661 accaatccta atgcaattga acttgcacac gataatgtac tcaccccata ttctacagct
    18721 tttatataat tattggtcaa aacagaatac atagtattat atattccata acctggtact
    18781 aatattatta aacagcaaac acttaatgta gttgctggag tcatataaat tcttgcacat
    18841 aactcacagt atatcgcaaa acaaactgta gctataaaaa aacataagtt ttctgttact
    18901 cccatattta aaagaaattt atacgaaaac caagctatac ttccacctat tcctgctagt
    18961 attaagtttt ttcctttcat attaaatagc actccaaagc ttaaggcagt aaaaaaagct
    19021 gctattactt ctataaacaa atgcacttat atccccccta acttgaagta caatccaagt
    19081 accaagaaca ttcctactgc tatagataca cctacaaaaa atacttctgc taattttgca
    19141 agaccagaaa tcaactgtcc atctaacaaa tctcttaatg cattagtcaa agcaagcccg
    19201 ggaactaaga gcattattgc tcctgctatt aaatggtcta acgtctttat gaatccgagc
    19261 tttaaaatta aaattgaaca agaagctata ataactgctc caattgcatt tacaaaaaag
    19321 tcattaaggg aagatttact taaatacata ataacaaact tagtcagaat cccaacagta
    19381 aatgcagcta taaattcttc cacatttcct ccaaataata tcgaaaaagt tgctgctgca
    19441 atacctgcaa aaaatattgt tattaaatcc gagtaatagt tatcttcact tatttcatct
    19501 aaaattttct cacataattc aatatctaaa ttatttatac ttattgctct tgataatgag
    19561 ttaacttcat gaaccaagtt taggtctact cttcttgagc ttattctttt aattgctgaa
    19621 tgtaatttcc catctacaaa tacagatgcc attacagctg ttggagttgc aaatacatct
    19681 acgtggtcta ttccaaaaga ttgacatatt ctagctatcg tttcttcaac tctataggtt
    19741 tctcctccac tttgcaacat agctttacct gcatttgagg aaaagtttaa aattctatct
    19801 atatccacaa aacatatctc ccattctttt atagtattat tcatatttta atacaagata
    19861 ttatttattt aaactatttt aaataattca tatccaatat ttacatttta tgaaaaaact
    19921 tgttttttat gcttgtcaaa atcaaatttt aagcttgttt acaccataaa atggctgaat
    19981 taatatttga caaacacttt caaccattta tataaccttc taaattgtat aactttttac
    20041 tacaaataaa attatatctc ttttaattct ttctcttgag tatttagctt atagtaactt
    20101 tatatttata tatgtacaat ataaaacatt atttttatga tacaaaata
//
LOCUS       DAENCV010000064        19449 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAENCV010000064 DAENCV010000000
VERSION     DAENCV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.065"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            114..1487
                     /locus_tag="KQD82_002354"
     CDS             114..1487
                     /locus_tag="KQD82_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4380822.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1743..2810
                     /locus_tag="KQD82_002355"
     CDS             1743..2810
                     /locus_tag="KQD82_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG4380823.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2954..3262
                     /locus_tag="KQD82_002356"
     CDS             2954..3262
                     /locus_tag="KQD82_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG4380824.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3255..4685
                     /locus_tag="KQD82_002357"
     CDS             3255..4685
                     /locus_tag="KQD82_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG4380825.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4763..5137
                     /locus_tag="KQD82_002358"
     CDS             4763..5137
                     /locus_tag="KQD82_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG4380826.1"
                     /translation="MKKREDDFEVRRKHLQELSEDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5131..7335
                     /locus_tag="KQD82_002359"
     CDS             5131..7335
                     /locus_tag="KQD82_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG4380827.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7335..8744
                     /locus_tag="KQD82_002360"
     CDS             7335..8744
                     /locus_tag="KQD82_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG4380828.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8729..9793
                     /locus_tag="KQD82_002361"
     CDS             8729..9793
                     /locus_tag="KQD82_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG4380829.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9823..11244
                     /gene="nhaC"
                     /locus_tag="KQD82_002362"
     CDS             9823..11244
                     /gene="nhaC"
                     /locus_tag="KQD82_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG4380830.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11508..11972
                     /locus_tag="KQD82_002363"
     CDS             11508..11972
                     /locus_tag="KQD82_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG4380831.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12270..12632
                     /locus_tag="KQD82_002364"
     CDS             12270..12632
                     /locus_tag="KQD82_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG4380832.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            12700..13314
                     /locus_tag="KQD82_002365"
     CDS             12700..13314
                     /locus_tag="KQD82_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG4380833.1"
                     /translation="MNEKWLEWAIELQSIAQAGITYGKDIYDIERYERIREISAEMIA
                     YKTDISTEKVRNLFCNESGYQTPKLDTRAVIFENGKILLVKENTGKWSLPGGWVEVNL
                     SVKENTIKEVKEEAGLDITADRIIAIQDRAKHNLPIYAYGVCKIFVLCTVIGGTFKEN
                     IETTEFSYFSENELPELATEKNTEEQIKMCFEACRTDTWTVIFD"
     gene            13334..13958
                     /locus_tag="KQD82_002366"
                     /pseudo
     CDS             13334..13958
                     /locus_tag="KQD82_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860815.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            14569..15429
                     /locus_tag="KQD82_002367"
     CDS             14569..15429
                     /locus_tag="KQD82_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380834.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            15687..17801
                     /locus_tag="KQD82_002368"
     CDS             15687..17801
                     /locus_tag="KQD82_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4380835.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMVEKKTLEWSDVYPFIYIHATYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            18343..19296
                     /gene="blaCDD"
                     /locus_tag="KQD82_002369"
     CDS             18343..19296
                     /gene="blaCDD"
                     /locus_tag="KQD82_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG4380836.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7613 a         2247 c         3659 g         5930 t
ORIGIN      
        1 tcatcaatat atgataagat aaatacaata gcaacatttt attaattttt tgggttttta
       61 tatatttagg tactggggtg atatagttga accaagagat attttttaaa ataatgaatg
      121 aaatattgga aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa
      181 tctacaataa ttctgtacaa aaaatggaaa aaatgaacag acatgatatt ttaaccaatt
      241 cattttttga tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg
      301 aaaaaaggga agttatcaaa gacaatattc aaaaatatct ggataaaaat ggaaaagaga
      361 taacagcaat taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg
      421 aaatatcaaa gaatatgaca acgatagaaa atttactaaa cagaaattat aaaaaaaatt
      481 tagtcaataa agttagtagt agacaaaaaa agggttatga atttgatgat attttagggg
      541 aaagtaattt gattaaagaa gcaatagcta aatctaagaa agcttgcaag tctgatgctt
      601 cagtatttat atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcact
      661 atggaagtaa taggaaaaac tatccattta tagcacaaaa ttgtgcagct ttgccggaat
      721 cactattcga aggtattctt tttggaacta gcaagggagg atttacagga gcaatagata
      781 ggccaggctt atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca
      841 tgcctattat gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg
      901 ttggaggaac taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc
      961 caaaggtaat tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa
     1021 ttcatataaa aattcctcct cttagagata gaaaagagga catacctcta ttgtgcaaaa
     1081 aatttatttc aaagtataat aaaaagttaa ataaaaaagt ggtaggaatt tcagatgaag
     1141 ctattaaaat tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa
     1201 tttattgtac tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc
     1261 taaacgacta ttacaatctt aaagagaata aagagagtaa caattattct ttaaacgatt
     1321 tggaggacaa aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat
     1381 cattagaaaa ggcatcttac aatataacta aagctgcatc aattttaggt atgaataggc
     1441 aaaatttaca gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa
     1501 aaaaacaaaa tcaaagtaaa ataaaaaagc aaaatagagg agcaaaatta ttataaaaga
     1561 gcaaaatatt ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa
     1621 atacataata aaataattat agtttcaacg ttccaataaa tatttacttt aattttaaag
     1681 ttggcacgat ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat
     1741 atatgagaaa agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta
     1801 atatgattct aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag
     1861 ggaaaagtat gtatgatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca
     1921 ataaaaatta tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg
     1981 attcatttac taaaaaggct tttgataaaa taattttctt attaaataga aaaataaatg
     2041 ttatatcaac agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa
     2101 aaatggatga agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag
     2161 gatttgtatt agatttacta gtattggcac tttcaggtac ttgtgaagaa gttactagta
     2221 taaaagctaa gagagtaaat gatttatctc catttggaaa gtctgttatg gtggaacaag
     2281 gtgttggagt aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc
     2341 atgtaggttt tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata
     2401 agatagaaca aacaaaagag ccaataatga ctacagtaga tagaaaatct aagtacggag
     2461 aggcattggc tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag
     2521 aagttttaat tgaaatggag catccacaac agataatacc agaagcagaa ggtattaaaa
     2581 caggagacta tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa
     2641 tacctggagg agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg
     2701 ctagaccagg tcttaaaact atgttggata taccagtacc tagagcaata atgggagata
     2761 taagaaatca gatagaagtt gaattggaag aagaatcaaa ggctaattag taagataaca
     2821 gctaactaat tagtaagata gcaactagct aattagtaag ataacagtta gctaattggt
     2881 aaaataatgt ttaatttaaa ataacagtaa attaattagt aaggcaatag tcaattaata
     2941 aggggatgaa aaaatggatg ctaaaattaa tgattgggtt attatacaca atatagtatt
     3001 gactccagaa gaaagagctc cacaagttcc agaggatact aagaaagtat ccttagaaat
     3061 gtgggttaaa ggttttatac aaagtgatgc ttctataggc gacttagtag aagttaaaac
     3121 aattacaggt agacttgtaa aaggtaattt acttaaagta aatccatatt atactcatga
     3181 ttatggtaaa tgtataccag agttactcca aataggaatc caagctaaag aaatattatt
     3241 tggaggtgta tataatgaat aattcaacat caatgaaaga tatgagttat caagctgtta
     3301 tgggaagaaa taacgaaata atgaaaaatg caatcggatt ggattattct tcttttgaac
     3361 aagagggaat agggtttgat tatgagaaaa tgatgagtga aacaggatat acattacaag
     3421 atattgaagc aattcaatca cagtatgctg ttggaaatac tcctttaata gagcttaaaa
     3481 atttgactaa attagctaga aaatgtgcta aagaaggtaa aggagctaga atatttgtaa
     3541 aagatgaagc aatgaatgca tctggtagtt ttaaagcaag aagagcagct actgcagtat
     3601 atcatgccaa acaaatggga tataaaggtg taatagcagc tactagtggt aattatggtg
     3661 cagctgtagc atcacaagcg gcaatgcagg gattaaaatg tataatagtt caagaatgtt
     3721 atgattctaa tggtgtaggt caacctgaaa tcatagaaaa agcgcgaaaa tgtgaggcat
     3781 atggagcaga agttgttcag ttaactgttg gaccagagtt attttatacg ttcttagttt
     3841 tattagaaga aacaggctat tttaacgcat cattatattc accatttgga attgcaggtg
     3901 tagaaacttt aggttatgag ttagctatcc aatttagaga aaagtataaa aaagaccctg
     3961 atatagttgt ttgtacaaat gcaggtggtg gtaatcttac tggaactgca agaggattga
     4021 taaaagctgg ttctattaat actaaagtag taggagcaag tgtagattta aaaggattac
     4081 acatggctag tgataatcaa tttaataaaa aatcatttac aacaggacat acaggttttg
     4141 ggataccata ctgtacatgg cctgataggt cagatgtacc taggtctgca gctagaccac
     4201 ttagatatat ggatagatat gtattagtaa aacaaggaga agttttttat acaactgaat
     4261 tattggcaca gctagaaggt atagaaagag gtccagcagg aaatacatct ttagcagcag
     4321 cattctcttt agctcaagag ctagatgagg ataaagctat agtggtacaa gaaacagaat
     4381 atacgggtgc aggtaaacat atatgtccac aacttacctt tgcaagagaa aatggaatag
     4441 atataaaatt tggaaatcca agagaagaaa ttgcaggagt aaacttaata ttaccagaaa
     4501 gaccagaatt attgaaatgt gttgatattg atatgaataa aataagaaag tcatttataa
     4561 aaaattgtat atctaataat cacattgata atattgataa gctaagtaat cgagatatag
     4621 aatttttaat ggaagaagta aaaagttcaa gagattttgt tgttgatgta ttaaataatt
     4681 tatagtaatt attgtttttt aatttaatat aaaataaaaa ttcagaaaat tcgattaaat
     4741 caatacgaat ggaggaatgt tcatgaaaaa aagagaggat gattttgaag ttagaagaaa
     4801 acatcttcaa gaactgagtg aggatgagtt aaaagaaagg ttttggagtt tagctaccca
     4861 gatagtagaa ccaatgatag aacttggtaa aaagaataca acaccatcaa tagaaaggtc
     4921 tgtcttgttg agaatgggat tttcttctct tgaagtaaaa cctattttag aaggggttat
     4981 ggaaagaggt cttataggta aaggagcagg gcatgtagtt tataaattgg ctaaatctaa
     5041 aaatataacg gttagagaag caggtttgtt attagttaag ggtgaatatt gggatgaagt
     5101 tacatgttta tttagagaag gggtagaatc atgttaaagg aaaataaaaa attggatata
     5161 gactatatat taaaagattt ggataaatat aaaccaaaac gaagaggttg ggtttggaga
     5221 gaacacttgg aaaatcttga aatgggacct tttaaatata aagactgtac aaaaccttta
     5281 aagaagagtg tagggctacc atcatctaag tatttcaata atatagaccc tcaaccaagt
     5341 ccagttatta ctactgaaat agcttctggt agatttgagg atgatataag aagaatgaga
     5401 atggcagctc atcatggagc tgaccatcta atggttatca gaactgctgg acaatcacat
     5461 tttgatgggc ttatagaagg aactccacaa ggtataggtg gagttccaat aacaagaaaa
     5521 caagtaagag ctcaaaggaa ggctcttgat tttatagaag aagaagttgg tagacctatt
     5581 aattaccatt catatgtaag tggtgttgct ggtcctgaag tagcagtaat gtttgctgag
     5641 gaaggagtca atggagcaca tcaagaccca caatataatg ttctgtatag gaatataaac
     5701 atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa taatggcttt tgccaatata
     5761 gctcaaattg atggagcaca taatgctaat gctactgcta gagaagcatg gaaggtaatg
     5821 cctgaactta tggttcaaca cgctttaaac tctatattct cagaaaaaat aggtatagat
     5881 aaaagtaata tatgcttatc aacagttcca ccaacagcac ctccagcacc atgtttaaaa
     5941 atagacttac cttacgctgt tgcacttaga gagttattta gtgattataa gatgagagct
     6001 caaatgaata ctaaatatat ggaatcatca actagagaag caacagttac tcatgtatta
     6061 aacttattaa catcagtatt aactagagct gacatacaat caactataac acctgatgaa
     6121 ggtagaaatg taccttggca tatatacaat atagaagcat gtgatacagc aaagcaagct
     6181 ttagttggta tggatgggct tatggatatg atagaactta aagatgtcgg tgaattaaga
     6241 gacaaagcaa gagaacttaa agaaagagct gtattatata tggaagaaat atctgaagtt
     6301 ggtggatatt ttgaatctgt ggaacaagga ttttttgttg attctggaaa ttatccagaa
     6361 agaaatggtg atggaatatc tagaaaaata aaaggtggag ttggtgaagg tactgtttat
     6421 gaaagagaag aagattattt agcacctgta actgctcact ttggatacaa taatgtagct
     6481 caatatgatg aaaatgctat agataatcca tcaatgttaa tagatggatg tacatttgaa
     6541 aatccagaca aaataatata tattgatgag cttgacgatt ttgataatgt agaaaataga
     6601 ctaaaagaat catatgagta cagaaatgga actaaaatta agcctgaaat ggaatggtgt
     6661 gcagatggaa ttgtaatgat aacaatgatg cttcctactg ataaaagaac agcagagttt
     6721 gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa ttgaggtcat aagtagagaa
     6781 gttatgcatg aatctgaagg gactagaata gaagtgaaag gaagagttcc gtttgatata
     6841 gatttaaaca atttagtaat accagaagaa cctaaggtac taactgatga agaaataaga
     6901 gcagatatag aagaaaaacc tatgaaaata gtttcagcta cggttggaga agatgaacat
     6961 tcagttggtt taagagaaat aatagatata aaacatggag gtattgaaaa atatggcata
     7021 gaatgccact atctagggac ttctgtacct gtagaaaaat tggtagatgc agcgattgaa
     7081 ctgaatgcag atgcaatact tgcatctaca ataataagtc atgatgatat tcactataaa
     7141 aacatgaaaa aattacacga ttattgtgta gaaaaaggta taagagataa agtaatgatt
     7201 gcgtgtggag gaacacaagt aacacctgaa atagctgttg aacaaggcat agatgctgga
     7261 tttggaagaa acagcaaagg catacatgta gccactttct tagttgaaaa aagaagagaa
     7321 atgagtaata aataatggaa aatagcaaag tttctttaaa aatagatgtg ttggtagctg
     7381 aaattggaag tactactaca gtggtaaacg ctttccacga tattaatact aacaatccta
     7441 tatttttggg tcaaggtcaa gctccaacaa cagtgtttga aggtggcgat gtaagaaatg
     7501 gcttatcagg tgctattaaa gatttagcta ataaattatc tgtagatgat atagagtata
     7561 atgatatgtt tgcaacttct agtgcagctg gtggattaaa aatgactgtt catggattag
     7621 tctatgatat gacagtaaaa gcagctaaag aagcagcttt aggggctgga gctgttattc
     7681 gtcaaataac atcaggtaga attaaaagaa ctgatttaaa taaaataaaa gaaataaacc
     7741 caaacataat tttaatagct ggtggagtcg attatggaga aagagataca gctatttata
     7801 atgcagaaat gatagcttct atgaatctag gcataccagt tatatatgct ggtaatgttg
     7861 aaaatcaaga ggaaataagg ttaatatttg aagatactaa ttataagctt tatataactg
     7921 aaaatgtata ccctaaaata gatttattga atatagaacc tacaagaaga atcattcaaa
     7981 gtgtatttga agaacacata actacagcac caggaatgaa atacattaaa gaaatggtta
     8041 atgaaaatat tactccaacc ccaggagctg taatggaagc ttctaaactt ttatataaaa
     8101 atattggaga tttattaact ctggatgtag gtggagcaac tactgatgta cattctgtta
     8161 cagatggaag tgactatatt aataaaattt tggttaatcc tgaacctact gcaaagagaa
     8221 gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa tatagtagaa gttataggta
     8281 aagaaaactt acaaagtgaa ctatctatag atattgatgc agtaatagag aattatcctc
     8341 caataccaaa atcaaaagaa gaaatattat ttgttgagag gttgacaaaa gaagctgttg
     8401 ttaaagctat gctaagacat tcaggaaaaa taagaaatat ctatggaaca actggaaaag
     8461 tcaaaattgc agaagggaaa gatttaacag aagtaagata tatagttggt acagggggag
     8521 cacttactag gctccctagt agaattgaaa tattagataa tatgctcaaa tacaataaaa
     8581 ataatgaatt actatttcca aaggaaaaaa caaagatatt aatagataat gactatatta
     8641 tggcatctat gggtgtttta tctaaaaaat atgaagaagc atctcttaag ctcctgttaa
     8701 aaagtttgaa ttttgaggag gaaagattat gtatcctagg ttagaaatag atatagaaaa
     8761 gttaaagcat aatgcaaaat taatttcgca gatgtgtcat gaaagaaata ttaagatatc
     8821 ttttgttact aaatcatttt gtgcacaaaa agaaatagta gaagaaatat gtagtgaagg
     8881 aatagaccat atagcagatt caagaataca aaacttaaaa aatttacaag atataaatct
     8941 acctaagata ttgattagaa tcccaatgtt aagtgaatta gaagaagtta tcaattattg
     9001 cgatattagt tttaactcag aattaggaac aataaagaaa ttaaatgaat tatgtgaaag
     9061 taaaaatatt attcataaga tagttcttat gtttgactta ggcgatttac gagaaggata
     9121 cttttatgaa gaagactttt ttaataatgt aagagagata gtaagattaa aaaatattga
     9181 aataattggt atagctacaa acttaacctg ttatggagct attataccat ctagggaaaa
     9241 tataggcaga ctggtaagta tagcaaaaag aatggaagaa aaatttggaa taaatctaga
     9301 aatagtatct ggaggtaatt caagttcgat tcatctgttg ataaataata atatgccaga
     9361 aggtattact aatttgagaa taggcgagtc cattctactt ggtagagaaa cggcatacgg
     9421 tgaaaatata aatggaacat atcaagatgc atttaaatta atatgccaag tagttgaatg
     9481 taaagaaaaa ccatcagttc ctattggaga aataggagtt gatgccttta gaaataaacc
     9541 tgtttatgaa gataagggaa tcttaaaaag ggcaatcata gctataggta aacaagatat
     9601 aaatattgac tctttaattc caatagacac tgatataaaa atattagggg caagttcaga
     9661 ccacatgata ctagatgtaa gtaacacaaa atatgattat aaattaggtg acaatttaga
     9721 atttttatta acttatgggg gtatcatgtc atctagtacg agcaaatatg tttcaaaaaa
     9781 aattatagtg tagtgtttaa ataattagga ggaaactatt ttatgaaaga gcgagaatgt
     9841 agaaaagcaa gtctgtttga tgcaatgata ccaattactt gtttgatatt atttttaagt
     9901 ctaggagtat tagtgtatgg ttcatcacct catgtaccac taataggagc agcagcagta
     9961 gcaggacttg tggctgtata tagacttgga tttaaatgga aagagttaga attaagtatg
    10021 tttaatagta taaagatggc aatgcaagca atcttaatca taattataat aggcgtactt
    10081 ataggaactt ggatagtaag tggagtagtt ccatgtatga tttactgggg actaaaaatt
    10141 ttatcaccaa atattttttt agtagcatca acattagtct gtgcaatagt atctcttgcg
    10201 acaggttcat cttggagcac aatgggtaca gtaggagtag ctcttttagg cattggtcaa
    10261 agtttaggta tgccaattgg attaatcgta ggttctataa tatctggagc ttactttgga
    10321 gataaactat cacctttatc tgaaacgact aacttagctc cagcaatggc aggaacagat
    10381 ttgtttacac atataaaata tatgctatat tcgactatac catcattatt aatttgttta
    10441 gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag cactagatat aaaacaaata
    10501 gaattaatta ggtctacatt agattctaca tttaatactt tatcaccagt attattatta
    10561 gctccagcaa tagtaattgg attagttgta tttaaagttc ctgctatacc agggcttata
    10621 gtaggtgctg tattgggagt attgtttgca gtagtttttc aaggagaatc tatgaatacg
    10681 atattagatg cagcacaaaa tggatatgtt tcgtcaacac aaatacaaga ggtagatgct
    10741 ttattatcta aaggtgggat tatgaatatg atgtccactg tatccttaac aatatgcgct
    10801 ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg aagtagtagc gtcttcttta
    10861 ctaagattag caaagggtgt atttggaaca gtattatgta ctatggtaac ttgtacaatt
    10921 accaatataa tagcaggtga acaatatcta tctatagtta taccaggaag aatgtataat
    10981 aaggaatata aaaagagagg tatacatcct aagatgttgt cgagagcttt agaagatagt
    11041 ggaactttaa cttctccatt agtaccatgg aatacatgcg gggcgtatat aacagcaaca
    11101 ttaggtgtat ctgcactaac atatggtcca tacgctttat taaatattat aaatccaata
    11161 gtatctttag tattaattgc atgtaagttt aaaatagcaa gaatagaaga tgaacctgaa
    11221 acttgtttaa attttgatgt ttgatatttt aattgtttgt ataaattttt tatagaaaaa
    11281 gatttctttt agacttgcct aaaaattagg caagtctttt aagtatgttc tgattataag
    11341 agcactgtgt gacaagaatg atgagattgt ttttgctata ctttcgatat aagaaatgga
    11401 aggaggttat gttttatatt catatgaaat aattagttac acagtgaatt ttcgtataat
    11461 ttggtataaa aagattagaa atgaaaataa agtaatggag gaataaaatg actagaatat
    11521 ccaatataac aatagctgaa caatctgagt actgtttttt ggcaatacga aaaacaattg
    11581 actttatggt agagttttca gagttttcaa aacaaagttt tgaaaaaatt tctaaatatc
    11641 tggaggagag aggaatttta tcttcaggag caccgattgt atgttttcat aacatggatt
    11701 tagcaaaatt ggatgttgaa gtagggtttc ctgtagccac ttatatagat gggaaaaatg
    11761 agattctaca aaggattgtt cctgcacaga aaataataac tgcaattgat ttaggacctt
    11821 atgaattaca agaccctact ttagaagact tattttcatg ggcaaatagc aatggatata
    11881 aacttcatgg cgatatatat taccagtatt taaatgatat caatcgtcca gctagtgaat
    11941 atcttacgaa aatgatgtta cccattatat agaaagttaa agattatcgt ttgttagttg
    12001 ataattagaa gaacatttac tgctactaca taaattttat cctaaataat atcaaggtga
    12061 tttaatttat gtattctaat gtaactgtag ataatcaatt tataactaac ttagtgtaat
    12121 ttagatttaa tttattattc taatgtaacc ccaatatatt gttaaggtct acttaagatg
    12181 ctcaaaaggc tatgcttaat ttattgttat ataatttgta gagtggtata atttagaagt
    12241 gatactatat agatatatag ggggaaaata tgaaaatata tgatatatta acacgttttt
    12301 atattcaaga tatagaaaaa gccatacctt tttatgagaa tcttttaaaa gaaaaatgtt
    12361 ctttgcgttt ttcttataaa gaagttgggt tagaattagc gcaaatagga aatgttttac
    12421 tattatctgg ttctgatgat gctcttaaac catttataga aacaaaatct acttttatgg
    12481 ttgattctgt tgatgaatgg agaagttatc tattggataa tggagcagtt gtagtaagag
    12541 ataagaaaaa agttcctaca ggatataata tgacattgag acatccagat ggtacaatta
    12601 tagaatatgt tcaacataca aagcaggatt agttaaaaat atattttact actaagtaac
    12661 taattataat atattcttcg gaaaactgga ggtaatttta tgaacgaaaa atggttggaa
    12721 tgggcgatag aattgcaaag cattgctcaa gccggaataa cttatgggaa agatatatac
    12781 gacatagagc gatatgagag aatacgagaa atttccgccg aaatgatagc atataaaact
    12841 gacatatcaa cagaaaaagt aagaaatctt ttttgcaatg aaagcggata ccaaactcca
    12901 aaacttgata ccagagccgt tatttttgaa aatggaaaaa tacttcttgt taaagaaaat
    12961 acagggaagt ggtcgcttcc gggtggttgg gttgaagtaa atttatcagt caaggaaaat
    13021 actattaaag aggtcaaaga agaagctgga ttagatataa ccgccgacag gattattgca
    13081 attcaagacc gtgcgaaaca caatttacct atatatgctt acggagtatg caaaatattt
    13141 gttctttgta ccgttatcgg gggtacattc aaagaaaata tagaaactac ggagttttct
    13201 tatttctccg aaaatgaact tcctgaactc gccacagaaa aaaatacaga agaacagatt
    13261 aaaatgtgct ttgaagcatg tcgtacagac acttggacag tgattttcga ttaggttaat
    13321 taaggagaaa ttaatggatt acaaagaaat cgtctttgat gtagacggca cattgattga
    13381 tactgaatac gccattttac actctctaca agacacgata aaacaaatca atagaaagat
    13441 aattcctatc aaagaattgt cttttgcttt aggaataaca ggcgaggacg ctttacgtta
    13501 tttgaaaatc gaagatataa caattgctct atcaatgtgg gataggaata tggaaaagta
    13561 taagaataca gttcttgtgt tcaaagaaat aaaggaaatg cttactgaac tcataaaatt
    13621 aaatattggc attggaattg ttacatccaa aactcatgaa gaatttagaa acgattttct
    13681 gagctttgac atagcaccat actttaatac tgtagtatgc tccaatgaca ctctagagca
    13741 taagccaaaa gcagtaccat tattgaaata catggaactg acaaaatgtc aaaacaatga
    13801 actgctttat attggggata gtatatatgc aatgtgccta tcgtgcaaat gttgattttg
    13861 gactggtaaa atggggttct ggcgatagag taagcgctgt aatatctttt accacaccat
    13921 tggatttgat aaggcaacta tctgtatcaa gcaactaaca atcatagaaa atagcaaagt
    13981 cagccaagcc agtcaatggt caagatgaac ggcgcatttc atgtgccacc gttgacagca
    14041 ccgtctgatt ttgctgacgg gcaatcaaga tgggaaagcc ctaaaatggc ttcccgccta
    14101 tttcaatttt gagaggaaaa tccgtctgct ttttcgtgag tgtggctaca agttggagcg
    14161 taggatgagg gacgggagct ttcatataca catgtatgct gctgaaaccc gccctgcgct
    14221 tgtggcttca cgctatgcaa tcacagtcct gttttcctgc cgcttcaaat gcccttgcct
    14281 tccctgacgg caacggcatt tttacggcaa cgccaacaga gcgtataaca cactacactt
    14341 tgcaagtaaa gtcgtttgcc aaggggaaag cccctttgga aattccagac aacaaaacag
    14401 tctgaacacc tcgcgctttc cctgtgcttg atgtttagct tttatttgtt gtaagcagcc
    14461 ccgtttcgtg gtctgcgtgt agttccttgt aaactgacct aagattcatt aaatataact
    14521 aagttataat taatataatg ttgcatggat aaaaggaggg aagctgcaat gtcttacaaa
    14581 gtaattaatg tcataactgc tattgactat atagaggagc atttatcaga aaagctggat
    14641 ttagatatag ttgctaatgc tgttcattac tcaaaatatc acctgcatag aactttcaca
    14701 acttctgttg gtctaacaat gcatgattac ataaagcgga gaaagttgac agaagcagca
    14761 aaattattgg tattttcaaa gaaaccaatt attgaaattg cattgatagc tggatatgaa
    14821 agtcaacagg cattcacttc tatatttaaa gctatgtata aaaaatctcc aaataaatat
    14881 aggaaagaac aagaatttta tcctttgcag ctaagatttg ttttgaaaaa agataaccta
    14941 ttttcaaaga atgtactaga gttggaaaaa gaaattaaac ttgcaagtat gtctgacata
    15001 ccattatgga tgaatttagt gagattagtc atagatggat ttccaaattt gcaagaggaa
    15061 gaatatatat atcaattaga acagtatatt ttagaagaaa gagctttaat attaaaactt
    15121 aataatgtcg ctattgcaaa tatggtgttt aatagagaaa ctagaagtat tgattttttt
    15181 ggaatacatc cacagtatcg taattcagac attgcacaag tgtttttgaa gaaagttatt
    15241 gaagatttct tgatagatac agatatttca attactacct ttcgagaggg tgacaaagct
    15301 gatacaggac atagagatat gataaagaag ttgggctttg cagaagcaga attgcttgtg
    15361 gaatttggat atcctactca aaaatttatt cttccttgtg tagaagaaag agataataga
    15421 caaaattaat tgtagattaa atgaaaagtt taccacatat tttttgtttg aattatttta
    15481 tatataagag catttctgct tttaatgtat tttgcttgta agtgtactta tggtaataaa
    15541 tatagaaaga tttagttttt taaaaaagga actttaaagc taaaaagcta acatttaagt
    15601 taaatagcaa gaatgattaa aaactccaaa acacaattaa gtataatgat tcagtgtaag
    15661 tacttaatta acaaaggagg atttaaatgt ctgaaaataa acaagtagta aattgcgata
    15721 ttattgcatc taattctaaa cataatatgt gtgaatatga tgcagagtta ttaacttttt
    15781 ttccagatga gattgcacat ctaatagaga taaataacaa gctagacaat gcttttaaga
    15841 aagctgaaaa tcttgttgac aagcttgata aggattatat ggacgctaaa atgtatatgg
    15901 taaaaaatcg tggtgaaata gacccacatg aaatgtttca aaatgaacaa ggactaaaac
    15961 aaatagataa ctatggggct tttatggtaa aagttcgaga taagattaat aaaatcaaag
    16021 attctccata ctttgctaga atagatttta ggctgaaaga tatggatgat gaatctaagt
    16081 attatattgg tagatttgca tttgattatg aagatgagct tataatactt gactggcgtt
    16141 ctccaattgc cagtatgttt tatgattatg aaatcggaaa agctggatat gatgctccaa
    16201 taggatgggt ggatggagaa ataacaagaa aacgacaatt taaaattaaa aatggaaaat
    16261 tagaatatgc actagaaagt tccattaata tacaagatga tattcttcaa aaagaactta
    16321 gtcacacttc agatgaaaaa atgaagtcaa tcatttcaac aatacaaaag gaacaaaatc
    16381 aaattattag aaatgataaa gctgatacac tgattattca aggggtagct ggttctggga
    16441 aaacatctat agcattacac agaattgctt ttcttcttta tcgatttaaa gataaaatat
    16501 cagcaaataa tgtaattatc ctatcaccta ataaagtctt tggagactat atttccaatg
    16561 tccttcctga gcttggagaa gagccattat gtgaattaag ttttgaaaat atagcagagg
    16621 tacaattaga tagagttatt aattttgaaa gtgagaaaga cccattagaa ataaatgatg
    16681 ctaaatggtc tgaaagagtg cgttttaaat ctacacttga ttttgtaaaa cttattgatg
    16741 attatataaa acaaatgcca aataagattt ttattccaaa agactacact tttggaagtt
    16801 ttacagctaa aagtgactgg atacagagta gatttgaggc atataataga tatccagtta
    16861 agaagagatt agagaaggta gctgaagaca ttcattataa atttgaatca gataatatta
    16921 tggaagaaga tttaccaaag gtaaaaagta tacttaaaag tttgaatgga atgttgacaa
    16981 taaaaaatac tcttacacta tataaggatt tctttaaaca aatgaatata tcaaacatgt
    17041 ttgtgatggt agaaaagaaa acacttgaat ggtcagatgt atacccattt atatatattc
    17101 atgctacata tgaaggtata caagaagata agattataag gcatattgtt attgatgaaa
    17161 tgcaggatta tacacctatt caatatgctg taataaactt actttttaag tgtaaaaaga
    17221 ctattcttgg agattttgga caattggtta atcctaatca tactcataca ttagatgata
    17281 tgaaacagct atataatgat ggggaattag ttatgttgaa caagagttac cgttctactt
    17341 ttgagattat taatttcgcc aaaaagattc aagacatatc ttcattagag cctattgaac
    17401 gacatggtga agaaccagct ttattaaaat gtaataacga gcaagatgaa ataaataaga
    17461 taaaaataga aattgaagaa tttaaaaaga gtgataatgc gactcttggt attatattaa
    17521 agacagataa tgacgctaaa gctgtatatg atgcgttaag agaagaatat agtgttcact
    17581 taatatcttc tgaaagttct agctttacaa aaggagtatc cataacttct attaaaatgt
    17641 caaaaggttt agagtttgat gaagttattg taccttcagt aaataataaa acatattata
    17701 gtgattatga ccgcagtttg ttatatattg cctgtacacg agctatgcat aaacttaagt
    17761 tgacatatac ttgtgaattg actcaactta tagatatgta gattatatac ttaattatgt
    17821 aagtgtttac ttatataaat ataacctgtc aataaaattc attaataaat caataaaaac
    17881 aatatttgta aaagtaaaag ctataaatta tatattaaat tttataattt atagctttta
    17941 taatactgct tatcttaatc ctcccaatag caaaataatt ttaaacatgc catattttat
    18001 aaatgtaata acaattatac aattaaaata actttcccaa actaacttga catttaaaac
    18061 ttactattgt aatatttaaa tattacaata gtaagtttta aatgtggaat caattattat
    18121 atcacaaatt aaaaggagga caggatgtta ttaatagtta cttaattatc ttcgcaatag
    18181 gtttaatcaa cataattgta ctaggtgaag aaaatataag agatacagct attcatttag
    18241 tcttgtttct tataagtaga acctagattg gagtatatta caaataatgt ctactatttt
    18301 aatagtaggt tataaattta aatatgggag atgatataaa ttatgaaaag aaaaaagaat
    18361 tttatttgga tagctatctt attggttggt gtagttatgg taatgtatta tgtagggaaa
    18421 aaacataacg atataaatca aaaaactgat aagaattata taaaatctga attaaataag
    18481 tctgaattaa ataaatccaa aattgaaaac aataataaaa acaaaaaaga aaaaattaat
    18541 atggttgact atagtgattg ttttgaaggt attagtggag gagctatttt ttataatact
    18601 aaaaataaag aatataatat atataacaaa gaattaattg aaacaagaag gtcaccatgt
    18661 tctactttta aaatagtttc aactttaatt ggtttagaaa aaggggtaat aaactcaaaa
    18721 gaatcagtta tgggatatga tggtacagag tatccaaata aaaattggaa taaaaatctt
    18781 agtttagaag aggcatttaa ggagtcttgt gtttggtact acaaaaaatt gatagataaa
    18841 gttgatgcta aatctgtcca aaacatttta gatgatttaa agtatgggaa ttgcgatatt
    18901 agcgaatggg aaggagattt aaaaaatgga aagggacatt taaatggatt ttggttagaa
    18961 tcttcattac aaatatctcc aaaagaacaa gtgcaaacaa tggcaaaaat atttgaggga
    19021 gatacaaact ttaaaaaaga gcatattaat attttaagag atattatgaa aatagatgta
    19081 aatgataaaa acataaatgt atatggaaaa actggaacag gatttgatga aaaaaataaa
    19141 tgtgtggatg catggtttgt tggtatgcta gagcgtgaag gtgacaccta ttattttgct
    19201 attaaatcag atgattccaa taaagaaatt acagggccaa aggtaaagga aattgcaatt
    19261 aatataatta agaaatatta tagtgtaagg gaataaactt aattttattt gtaaatatta
    19321 tttacctata gtctgtaata tttattaaat aattttacta aattgagtct atatagagca
    19381 aaactccttt agtacataaa atacttgcta ttgattatta aattgaatac ctatagattt
    19441 aaagttaat
//
LOCUS       DAENCV010000065        19397 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAENCV010000065 DAENCV010000000
VERSION     DAENCV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.066"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(35..1063)
                     /locus_tag="KQD82_002370"
     CDS             complement(35..1063)
                     /locus_tag="KQD82_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG4380837.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1111..1971)
                     /locus_tag="KQD82_002371"
     CDS             complement(1111..1971)
                     /locus_tag="KQD82_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG4380838.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2240..2449
                     /locus_tag="KQD82_002372"
     CDS             2240..2449
                     /locus_tag="KQD82_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG4380839.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2449..2715
                     /locus_tag="KQD82_002373"
     CDS             2449..2715
                     /locus_tag="KQD82_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG4380840.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2744..3316
                     /locus_tag="KQD82_002374"
     CDS             2744..3316
                     /locus_tag="KQD82_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG4380841.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3550..4740)
                     /locus_tag="KQD82_002375"
     CDS             complement(3550..4740)
                     /locus_tag="KQD82_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG4380842.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5000..5431)
                     /gene="dut"
                     /locus_tag="KQD82_002376"
     CDS             complement(5000..5431)
                     /gene="dut"
                     /locus_tag="KQD82_002376"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG4380843.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5544..7772
                     /locus_tag="KQD82_002377"
     CDS             5544..7772
                     /locus_tag="KQD82_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG4380844.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHRECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7883..8368)
                     /locus_tag="KQD82_002378"
     CDS             complement(7883..8368)
                     /locus_tag="KQD82_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4380845.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8620..9843
                     /locus_tag="KQD82_002379"
     CDS             8620..9843
                     /locus_tag="KQD82_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG4380846.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9847..12981
                     /locus_tag="KQD82_002380"
     CDS             9847..12981
                     /locus_tag="KQD82_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG4380847.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            13015..14313
                     /locus_tag="KQD82_002381"
     CDS             13015..14313
                     /locus_tag="KQD82_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG4380848.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNSGK"
     gene            complement(14589..14834)
                     /locus_tag="KQD82_002382"
     CDS             complement(14589..14834)
                     /locus_tag="KQD82_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4380849.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15048..17675)
                     /locus_tag="KQD82_002383"
     CDS             complement(15048..17675)
                     /locus_tag="KQD82_002383"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG4380850.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17736..18578)
                     /locus_tag="KQD82_002384"
     CDS             complement(17736..18578)
                     /locus_tag="KQD82_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG4380851.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18585..19223)
                     /locus_tag="KQD82_002385"
     CDS             complement(18585..19223)
                     /locus_tag="KQD82_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380852.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         6757 a         3085 c         2677 g         6878 t
ORIGIN      
        1 aattgtcaag ttctactcta ttatttataa tttcttatat cttattaaca acatttaagt
       61 tattacattt atttatatat attttttcat tatctaagtc ttcatcaaac atttttatta
      121 tattttttat ctcatacttg tactcatctt cattcattct ataaagcatt aaatcttcta
      181 gtacatcttt catatcgaag tacacatctt ctaataaata aaaatagcct tcctctatat
      241 ctttcaattt atctatagta tccatttttt tacttatatc tttcataatt tctaaatttt
      301 gtatatttac atctatgatt gttaataagt acattctcac agtttttatt atttcaacag
      361 aaaaatctac aatattataa tttccttctt ttttatataa tgactcctta aatttgttaa
      421 aacaattatc taaattagaa tttatatcgt taaatggtgg tcttattatg tcacgtatta
      481 ttaaaagatt ttgtatatct acattatttg tgattgcctc atccaaaata aataaattat
      541 atgcatcatt taatgggtca tgggcatctc cactaaactc taagttaaca aattcaagta
      601 aatttttcaa tgatatatag ttcatacatc tcataccaca atttatatat ttttctttta
      661 tatctacaaa taaatctcta atgttattta caaaccttgg atggttattt tttttagcac
      721 ttctcatatc tgtgttattg aaacatgtca aatccaaatt accaaaagta tatattcctt
      781 taatatcaga aaatatgcca agccattttt taaactttct catgacctct tcataacttg
      841 gtgcactctt caattcttca gaagatatat gtgttaattc ttgtatatga ggatacaact
      901 cctcattact tactggtctt attaaagatt taaatttatc aatatttttt gttttttcat
      961 catacataac tgctccgatt gcaattatat cagaattaaa tactgctctt tttgatttag
     1021 aatttggcat attcatttca aaatctatat atactctttt caatataaac accctttcca
     1081 attgtttata gtgattcttt ttaactattt ttattcaaaa ttttctaaga aagaatgttt
     1141 ctctccatct ttttgccaac caagtactga atcaagattt acattttctg ccgccttaaa
     1201 tatagatttt tctacatttt taaaatttga ttctaaactc tttattaagt cttttatctc
     1261 atatctttta ttacctgttt ttttagcagc aaccatacat gcacaattta atggccatat
     1321 accactattt tgaataaatc ttataatatg ttcttctctt atataataaa gtggtcttat
     1381 aatttttata ccttcaaaat tagtagaatt aagtttagga agcattgttt taaaatttcc
     1441 agcacataat aagttaagca ttgttgtctc tattacatcg tcataatgat gacctaatgc
     1501 aagtttgtta caccctaatt cttctgcctt tgaatataat gctcctctcc tcattcttgc
     1561 acacatatag catggataat cttttgctat ttcatctgct atctcaaata tccttgaatc
     1621 aaataaatgt attggtatat ttagatactc acaattatct ataagtaatt gtcttatatt
     1681 tgcatgatat ccagggtcca tagcaataaa ttcaacatca aaattaactt gtccatgttt
     1741 ttttaactct tgaaacattt tagccataag aatactgtct tttccaccag aaatcgctac
     1801 agctatctta tcaccttctt caaccaattt ataatctctt attgccttca taaacttaga
     1861 ccataaattt ttcctatatt tttttattat acttttttct atatctctta ctggaagtct
     1921 ttcctcaaaa ggaactataa tttcacagcc ttttcctgat agttcactca tctaatcacc
     1981 tcgtatattt atgttaataa tattttacaa aaaagtctac cttcatccac aaaatactat
     2041 aatttttact ttaattttac taaataattt atagtttaca ataacacata aacttataat
     2101 aacataaata acctctttct tttattatct tttttatttt tctcatttaa tattttatta
     2161 ttagtaacga tttttaaatg attgaatatt atacactaga tagagttacc tatctattta
     2221 caacaaggag gttttcatct tggaaaataa gactagagat atcaaaaaaa attgtggtcc
     2281 atctttggct agaccataca ttaataatca aattttcaca gaaatgtact gcctttctga
     2341 tgcattaaaa tttggaacta tatttccaga actaaactta ctagagtctg aaatgtacaa
     2401 caaagaatta tatgctaagc caaaaaaata tagaggaggt agaagataat gaaagactca
     2461 tctagagctg aacttatgag aaaagttcag gaaacatctt ttgcatgtgt tgatatgaac
     2521 ttatatttag acaatcaccc tgatgataaa aatgctatca acacttataa ttcattatgt
     2581 aatcaatttg cgcaagctag atatgcttac gaaaataaat acggtccttt aactaacttt
     2641 gggtatgctc caagtagatg cccttggcaa tgggctgacc aaccttggcc ttgggacaga
     2701 gaatttaatt attagacatc aagtattgga ggattgaaaa ttcatgtgga tatatcagaa
     2761 aactatacaa cacccagtta atataaaaac ttgtgaccct agaatggcta aatttcttat
     2821 aactcaattt ggtgggccaa atggagaact tgctgcatct ttaagatatt taagccaaag
     2881 atatacaatg cctactggta atatgcgtgc acttttaaca gatattggta cagaagaact
     2941 agctcacgtt gagcttatat gtactatggt ttatcagtta acttctgatg caagcccaga
     3001 agagttaaaa gctgcaggtc ttggttcaaa ctatgctcaa aatggatatg gaatttatcc
     3061 aacagattca aatggtgttc catttgatgt aagacctata gcagttatgt caaatcccgt
     3121 aaccgattta catgaggata tggcagctga acaaaaagca cttgcaactt attatcaact
     3181 tataaaccta acagatgacg ttgatgttat agatgtatta aaattcttag gtcaaagaga
     3241 aataattcac tatcaaagat ttggtgaagc tttaatggat gcttacgagt tagaagaatc
     3301 tcaaaaaatg ttctaaaatt tttatttgta taactattat tatttgataa tttataattt
     3361 actgttgtgt taaaatttat aaaaaatata aatataattt attttctata aacagagaaa
     3421 ataaataaaa agcaggtatc aataaaatag taacatgtat tatactatac attactatca
     3481 atattgtacc tgctttcata ttataagcaa taaagaaaaa tctatttatt aggttttata
     3541 ataaaacaat tataaaactc ttcttgcatt tacatatcta cttgaatagt agctactatt
     3601 tatattagat attgttactt tttttgatga acctgaagat gcatgtatca tatctccatt
     3661 tcctatgtat attccaacat gacttacaga accattattt gaaccttgtg tatcaaagaa
     3721 tactaaatct ccacttctaa ggtctcctct atttacatat gttccatact tactttggtc
     3781 tctagatact cttggtatac taacccctgc agattttttc ataacgtact gtgtgaatcc
     3841 agaacagtca aaactatttg gaccttctgc tccccataca tatggttttc caagtagtgt
     3901 ctttgcaaaa cttattacac tatcagctcc ttgacttgga gaattagtcg aataagatgg
     3961 aattgaagta tcaacagaac ctttttcagc aaggtattga cttgacacat atccagtttg
     4021 attgccataa cttacttttg tccatcctcc actttcagaa atacttccaa cctcagtacc
     4081 ataacttaat gtagctattt tagaatgact tgtacctggt cctgttctaa cgttcaatga
     4141 ctttgctgtt acaacctttg taccctttac aaggtcattt gaactgtttt cagcattttc
     4201 atttgtagag tcatttacac tagttcctaa atatttactt gaaacataac ctactctacc
     4261 attagaactt atttttgacc atccatttga ctcagatatg tatccgactt cagtcccata
     4321 tcctagtgtt gatattttag aactacttgt acttggtcct gttctaaaat ttaatccttt
     4381 tgctgttaca acttttgtcg acttaactga agtatcacta ctttcattgc tacttccaag
     4441 gctatttatt gctacataat ttccatgcac ataacctgtg tttccattat atttaacttt
     4501 tacccaagaa ccttcttttc ctacgtactc aactttatca cctttgtaaa gtttccccat
     4561 tgattcatgg tttgtacctg gtccttttct aaaatttacc gcattccctg ttattgttct
     4621 gctttctata ctcacactct ctacttcaga ctcattagca tatgctaaat tccccatagt
     4681 aggcattatt atcgcagtcg ccattatact ggctgctatt ctcttttgat taaaattcat
     4741 aactttccac tcatctcctc ttttctacta tctttatttc acttcaaaat ataaaagaaa
     4801 taattgacac tattaaaata tcaattattt cttttatatt aatattatta caaatttgta
     4861 actatttgtc tatagtaaca tttgcttacc ataaattacc atgtataaat tttaatcttt
     4921 aatagcctgt aatttagtaa tatagccatc ttttaccttt tttaaaaatg gctatatttt
     4981 ttaacttttt tgtaactttt taaaatccag ttgagccaaa cccacctttt cctctttcgg
     5041 agtcacttaa ttcttttact tcttctatgt taatatcata gataggtttt actatcattt
     5101 gagcaatttt catatgcttt tcaaccttaa aatcattttt accatgattt attaatataa
     5161 ttttaagctc tcctctgtat ccttcgtcaa tagttcctgg tgtatttaat acagtaacag
     5221 aatgctttaa ggcaagccca cttctaggtc ttacctgcgc ttctgtcata gtaggaagct
     5281 ctatacatat cccagttttg ataagtttag tctctccagg aggtattaca acctcttcta
     5341 tagaatacaa atccatacca gcatcacctt tgtgagcaaa attaggtatt atagcatcat
     5401 catttaattt ttttactttt aaattgtaca tcttatatct cctttcaact ttctataaat
     5461 attataactt aaatttcgac ctcttgaagt tttttatttt tttgtggtaa tataattatt
     5521 agtgttttaa aggaggattt tttatgaaac ttgtacttgc cgagaaacca tctgtggcaa
     5581 aaactatagc atcattttta ggtgcaaaga ccagacaaga tggttacttt gaaggaaatg
     5641 attatataat tacttatgca gttggacact tagtatcatt atatgatatg aaggattatg
     5701 ataaagataa atactctggc tcttggaaaa tgaacaattt cccatttgtt cctgaggata
     5761 agtttaaatt taagattgat agttctaaaa ctaaacaatt caacactgta aaaaagcttt
     5821 tgaatagaaa agatgtagaa tatgtaataa atgccactga caatgaccga gaaggagaac
     5881 ttatagcatt tttaatattt ttactggcaa aaaataaaaa accagtaaaa agaatcttgg
     5941 taaatgaatg gacaccagaa gatattacaa gaggtataaa aaacctaaaa gatgaagatg
     6001 aaatgagaaa tcttcaagct gctggatata ctaggcttat tactgactgg ttgattggaa
     6061 taaatttcac ttctgttgct acattgaaat atggaaatgg gaaactatta aacataggta
     6121 gggtaattct tccaacagtt aaattagtct atgatagaga tatggaaata ctaaactttg
     6181 ttcctaaaac ttattatgaa atagaagggc attttaaagc tgaagctggt gaatataaag
     6241 gtaaatatgt taagggtaaa gaaagtaaat ttgatacact tgaagatgca aataaaatta
     6301 tagcttcaat tacatctgaa actggtaaaa tactagataa aaaagttact atgtcaaagg
     6361 aatatgcacc aaaacttttt agtttgacat cacttcaagg atatataact tctaagtatt
     6421 ccaattttac atctgataag gttttaaatg tctgtcaatc tctctatgag ggtaatggta
     6481 agggtggata cataacttac cctagaactg attccatttt cttagaagaa agtttggctt
     6541 caaaagcttc tcaaacttta gataaattaa aggttggtct tgaatatgaa aataaaatta
     6601 aattttctaa aactaaaaga gtttttgatt cttctaaagt agactcccat agtgctataa
     6661 ttccaactta tataattcca aaaagtttat ctaaggatga acaattagtt tatgatgcca
     6721 taaaagatag atttgtagca aattttatgc ctcctgctga atatgagaat acagaaatta
     6781 agactgaagt agataattgt actttcttaa caaaaggtaa ggtgcttaaa tcaaaaggat
     6841 accttgaggt atataataag gaagaaaaaa acgatttact tccacttgta aataaagatg
     6901 atgttgtaga tgtgttagaa ataaaaccat taactaagca aactactcca cctaaaccat
     6961 atactgagga caccctttta aaggctatga aaaattgtgg taaaaatgtg cctgaagaag
     7021 atacaacagt gctatctgga tattcgattg gaacatcagc cacacgtgct gatgtgttaa
     7081 agaagataag tcaagtaggc tatgtaaaga aaaaagggaa atcttattct ataacagacc
     7141 ttgggaaaaa tttagttgaa atttttcctg ttaaagactt atttgatgta gattatactg
     7201 gaaaacttga aaaaagttta agcgatattc aaaaaggaaa gtatacaaga aaagaatatc
     7261 ttactaatat cattagtttt atatatcaaa atgtcaatat tataaaacaa gatgcttcaa
     7321 aaaatataaa cacagaagca tatactttcg attcaaaaac taaaaaattt actaaaacaa
     7381 aagaaaaaaa acagaaaaaa gatgtaacta atactgaaaa agaagttgct atatcaaagg
     7441 attctaataa caaaaaagaa gttaaaaatg gtgatgaaaa tcaatctctt ggcaaatgcc
     7501 ccatttgtca aggtgatgta ctagaatttg ataaaggctt tgcttgtaaa aatcatagag
     7561 aatgtaaatt tgttatttgg aaaaatgata agtatttaag tctttataaa aagaaagtta
     7621 acaaaacaat ggtcaaaaat attttaaaaa aaggtgaaac taaagtaaaa tctttaactg
     7681 ctaaaaacgg taataaattt gatgctacat taaaatatga taaaaatcca gatactggct
     7741 atttcaattg gaaaatagag tttgacaact aaatattcat aagaaaagaa atagcagtag
     7801 tataataatc tatattatat tactgctatt tcttttctta tgaagtttta acttttttaa
     7861 tagtgacttt tacaatcaca ctctaaccta atttctttac tatttctgat aactttaatg
     7921 tatgttcatc taataaagtt agttcctctt cagaaagatt agataatttt tttactagag
     7981 attctttgag aatagctttt actgcatcaa ataatttaaa tgctttatct gttaattcaa
     8041 ccctaagaat ccttctatct tttgggtcag gaaatttatt gactaaacca tgttccacta
     8101 atctgtcaat aatcggagtc atatttgatt ttaaaattcc taaattagaa gctatttgag
     8161 aaattgatga tgaatttgat gtcataagat aaagaattac cttagcatgt gatggagcca
     8221 ttggacattc ttcaaaataa gctttcatta tatcagagtt attttgaaac ttttttaaca
     8281 aatcgctttc tttaaatata ttattttcaa gcacaaataa aaaatctaaa aaagagtccg
     8341 ctattttaag tatattgttg ttatccatat ttgtaacatc ccttcaatag ataatataat
     8401 aaaacattgt tgtttaatat agtataaatt ataaactatt aataaattta aaacaagaaa
     8461 aagttattga ctcataataa ttataaattt ataatcaaat tagagaatta taaattgcca
     8521 ataaagggaa gttttttaat tagtctaaaa ctaaacttaa attataagac actttaaatt
     8581 ctacaacaag ctagtgtgca atatataata ggaggagata tgaaaagata tttaatagtt
     8641 ttcttaatgt gtagttcact gtttttagtt ggatgtggaa aaacagaaga atctgaacct
     8701 gagaaaccaa tagcagtttc tgttcaaaaa gcaataggtg gagaaattga aaacacaaat
     8761 tctttctcag gaactacaaa ggttaaagat gaaacagctg ttactgcaca gactgttggc
     8821 acagttcaag aagtgtatgt aaaacttggg caaaatgtaa gaaaaggaga tgaattattg
     8881 agtataagca gtccagagct agaaaatagt gtaaagcaaa gtaaagcctc actagattta
     8941 gcaaaagcaa gttattcaag tgccactggt ggttcattag aggcacaagt aaatcaggca
     9001 aaaacagctc ttgacaatgc aaagattcaa tatgaggagt ctcaaagaaa ttatgacaat
     9061 aataagattc tttatgaaca ggaagtcatt agtttagacc aatttaaaaa aatagagttt
     9121 tctttagagc aaacaaaaca acagttagat tctgctcaaa gagcatacga tacagcaact
     9181 tcaaaaagta tacctcaagc taaagcactt gctaaaaaac agcttgacca agctcaagta
     9241 tcatataatt tagcaatgag taatttagac aaattaacat taacatctcc agtagatggt
     9301 acaataacag ctaaaaactt tgattcaaag gaaatgataa cacaatcaca accagctttt
     9361 attatttcta atcctaatat actagaagtt gatttaaatg tagcagaatc agatataggt
     9421 aaatttaaga aagatggaaa tgtagatgtt ataattgaag accaaaggat tttaggaaaa
     9481 atagattatg taccttcagt agttgaccct cagacatctt tatatcctgt taaagtactt
     9541 gtaaataata caaacaacaa atttaaggca ggtatgtctg ctcaagtaaa cttaagtgtt
     9601 gaaaaagaaa atggtgctgt tacagtacct aaaaaagcaa tatttgaaga aaatggtaaa
     9661 aaatatgtat acatagcaac aaaagacaat atagcaaaga aacacttggt tcaaactgga
     9721 attgtaacag aggataaaat agaaattaaa agtggtgtaa gtgataaaga tacagtgatt
     9781 attggaggga taagtcttat ttctgacgga actaagatat ttcctgtaga aaaggagaag
     9841 tagactatga atttaacaca gacttccgtt aaacgtcctc tcacaatcat aatggtcttt
     9901 ttagtattaa tagtttttgg tggtattgga tataaaaaaa tgtcaataaa tctaatgcct
     9961 gatatagaaa taccagttgt aatggtcatg actacttgga caggtgctgg tcctcaagac
    10021 gttgatgaac aagttagtca aaaagttgat gaaagtttat ctgccgtttc aaatgtaaaa
    10081 tctactatat caagttcaca agaaagtgtt tctatggtag ttgctcagtt tgaatttgga
    10141 actaatttgg atgaaataat gaatgatgtt aggtctaaag ttgatgcatt acagaccagc
    10201 ctcccagatg atgcagctaa accaacagtt ttaaagctgg atatgaatgc acaggctata
    10261 ggtcaacttg tcatatctgg aggaaatgaa aatagttcac aagccttaag aaaatacgct
    10321 gaagatgtaa ttcagcctaa aatagaatcc atagatggtg ttacttcagc agaccttaaa
    10381 ggtggagaaa aagcacaggt taatgtaata gctgacccag ctgttctttc aaattatgga
    10441 gttagtttat ccactataaa aggtgtctta tcctctagta ataaaacatt cccttatggt
    10501 tcaattactc aaggtgagga taaaatagtt ttaagagcga ttgataaatt agaatcccta
    10561 gatgatataa aacagattca aattcctgta aaaggaggaa atacagttag attagataat
    10621 ctttgtagtg tagattatgg atatgcagac aaagatagta tttatagata caatggaaaa
    10681 gacagtttgg taatagatat ttctaaacaa caagatgcca acactgtaaa agttatgcag
    10741 agtgcataca aatacgtaga tgaattgaat aaggaaaatc ctcaatttaa aattaaaatt
    10801 gctaatgata caagttcata cataagtgaa tcaataaaca gtgtaatgag caacttgctt
    10861 ataagtgctg ttatcgcatt tattgtaata tttgctttcc taaaaagtgt aagagcttca
    10921 ctagttgttg cagttgcaat accaatttct ataataggtg ctatagcaat tctctacttt
    10981 actggtgaaa gtttaaatct tattactgca agttcattgg taatttcagt tggtatggtt
    11041 gttgataatg ctacagttgt aattgaaaat atatttaaat ataggaaaag tggcagattg
    11101 agtcttgatg attgtgctat tgagggaaca agaactgtaa caaatgcaat acttgcatca
    11161 acattgacca caatagcaat attcttacct atcatgttta cagaaggtat agctaaaata
    11221 acttttgggg cattaggtaa aacactaata gcttcactag cattctcctt tataggtgca
    11281 attacactcg ttcctagtgt atttgcaaaa ttaagcagag gaaaaaatag tcaaaagatg
    11341 caagaaaaag atagtcctat atttgataag gtaagtgaag catataaaaa attattaagt
    11401 gtatgtttga gacatcaacg cttagttgta atatcaagtt tacttatttt tgtagcttcc
    11461 ttatttggaa tgggatttat aggtatggac tttatgcctg cagcagacaa gggtgaatta
    11521 tctatttcta tagaacttcc aaaaggtctt agcctagaat ccaatgatta ctatgtatct
    11581 atgactgaga aaaaagtctc agatattcca gaaattaaaa ctttaataac aactttgaca
    11641 tcagattcaa ataatagtaa agcaagtata gcaattgaat tagaatctca aaaaaataga
    11701 aaaagaacta ctgatgaaat agaaaaagag ataattgata gagtggctac tgtaccagat
    11761 tgtaaaataa atgtaagtca aaacagtagt gttatgggtg gagctggagg agcagacttc
    11821 acattagaaa tgaaaggtcc tgatttagat gtattaaaaa tactgggaaa agaagctgaa
    11881 aaagaattta gctctataaa aggatttaga aatgttgaaa cttctcttac agatacaaca
    11941 caagaagctc aatttactat agataaacat aaggctcaag aatatggtgt aaatactgct
    12001 gaaattgcag gcatgttacg tacagcaata aatggtgact ccgtaaccac tgctactatt
    12061 gatgattaca aagtggatgt aaacctaaaa tttaaagcag atactattga tagcttagaa
    12121 gatataaagc aactaaaagt aaactctgca actggaaaag aagttcctct tggtgctttt
    12181 tcagatataa aaatggcaga tggtctacaa tctatatcta ggactgatgg agatttttct
    12241 gtaaatataa ctgctacact tgacaattta gatgctggta ctgctacaaa actagcacta
    12301 gaaaaaacga ataaaatggt aattcctaaa gattactcta taggtgttgg tggtgaaaca
    12361 gaaatgatga atgaatctat ggcaagtctt ggattttcaa tggtcatagc tatagcatta
    12421 gtttatatgg taatggtagc tcagtttgaa tcatttagta aaccatttat aatcatgttc
    12481 tgtgttccat ttgcattcgt tggagttgtg ctttcgcttc tagttagtgg tcaaagttta
    12541 agtactgtcg gtatgctagg tataatctta cttatcggta ttgttgttaa taatggtata
    12601 gttcttattg actacataga gcaacttaga aaaacaaatc gtaacaatga tttaattggt
    12661 ttggtttcaa ttggttgtgc agcaagatta agaccagtct taatgactac agcaacaaca
    12721 attttaggta tgttgccaac agcactatct cttggtgaag gtggagaaac tatgcaacca
    12781 ctagccatag ttataatggg tggtctaact gtatctacac ttataacttt ggtgcttata
    12841 cctactatat acttaatatt tgataaatgg gaaaataagt tttcagacaa aatgcctaaa
    12901 atatttggta agataacaga taaaatagga agtataaaac gtaaaataat aaagaaacca
    12961 aaattgaaag ctaaagttta aatagaatgg agggtgattg tattgaataa aaaaatgagc
    13021 aaaatagttg caattggctg tggtgttggt ataataactg ccaatttaat cccagtttac
    13081 gcagataata atacttcttc tataaacaaa gttgataatt ctattttaag tgactcaaat
    13141 aaaagaacat ctgaaaagga aattttgact gctagagaag ctgttagtgc tgcacttaaa
    13201 aatagtgaaa aattaaaaat gaaatcagaa gaaataaaaa tgttaaaaga aaagttagaa
    13261 gtacaagatg agtttgatag ttttactgga agtgacaact cgtttccata cgaccaaatt
    13321 ggacttctta aaaatcaaag tgaacaggct aaaggcttta tggaggacca aatagcaaat
    13381 gatattacta ataaatttaa cgatttagtt tcaagagaaa atgagctaga taaaataaaa
    13441 aacaatcttg aaataaaaac taaagaaatt aaagatatga aattaaaaaa agatttaggg
    13501 ttagtaacat ctttagaaac tgaatcagct gaacttgaac ttcaaacact acaaaatact
    13561 caaaaagcta aacttcaaga actaaaaaac aatcaagact attttaagtt attaactaat
    13621 atagatttaa ataattatca attggataaa gaatatagat ttgaatcttt tagagtaggt
    13681 ggctcagttg attcatatat ggaaggtaag gttaatgaat atttaaaata cgaccaattg
    13741 attttagaac gtacagaaga aagctttaat gataaagatg agaacaaagc agatttacca
    13801 gatagaccta accttacaag accagttgct cctacaaaac cagagcaagg taacctaact
    13861 gatgatgaat acaaggttct tatggataag tatgaaaaag attatgatag atataaaaaa
    13921 gaagttgaag catacaatct tgaaagacag acttatgctg ctggtcttac tacttatgca
    13981 aattatcttc aacaaaaatt taatactgaa aatggtctag taacattaga agacagtaaa
    14041 aaagctctta aaaaagggtt aattgatagc tatgctcaat tacttgcttt agaagataca
    14101 attcagatta ctaaaaaaca acttgattta tcagaaaaac aattaaaaaa tactaaatta
    14161 agatatgatt taggattaat tactctaact gattataaaa aacaagtggt aagtaatgaa
    14221 gatgctaaaa atagctatga ctctcttatt gtgaattata actcactaaa aaatggtata
    14281 gaaaaacctt ggatacttaa ttctggtaaa tagaaagatt actttaacat acgtttgatt
    14341 aaaaaatagt tatgcacact cgtggcttta gtcatggaag attcagaagg caaataatag
    14401 aaatagatta gtaaaaatat atttacttat aaaactgtat acttttatag aggctatctc
    14461 aaaatagaga taagcctctt ttttattgtt taaattataa aaatatattt gattccatac
    14521 aaaaagagtg ccttatgaac taaaaagttc attttgagac accctcaaaa gcttaactct
    14581 aaatttaatt attttttatc aaatgtctca tctattttac atctcaccat gttaacaaat
    14641 tcagaaatat tatatcctct aagtttgcat ccacatttag gacaataatc cagtttccta
    14701 gataccaaag ccctacaact aggttctgga catcttattt ttagactctc atctgctact
    14761 ttaaatccac atcgtgggca tatactacct ggacgtgatg atacttcctt tccacattta
    14821 ggacactttg tcaactttat cccctccagt aaactcaatt taattgatag ttgaaatttc
    14881 tctctacaat aatatatgca atataattaa atacgtttca cttacaacta tataagttaa
    14941 atttttataa taaaaatccc ctatattcaa tttacaaata cttaatttga tacattgaac
    15001 aataggggat tttattttta tttagttaca tattttgcag tctattatta tctaggattt
    15061 tttatagatg cttgagctgc tgcaagtcta gcaattggca ctctaaatgg tgaacaagat
    15121 acatagttaa gtcctacact ataacagaac tctactgaag atggttctcc tccatgctct
    15181 ccacatattc ctagttttaa ttctggttta acttctctac ctaatttagc tcccatctta
    15241 actaacttac caacaccatt ttggtctagt acttggaatg ggtctttttc taatatttct
    15301 tcatctacat attgacctaa gaatttacca gcatcatctc ttgaatatcc aaatgccatt
    15361 tgagttaagt cattagtacc aaaactgaag aagtctgctt cttgtgctat ttcatcagca
    15421 gttaaacaag ctctaggtat ttctatcata gtacctacag tataaggaac ttctatacct
    15481 tcttctttta ttatagcgtc aacagtttct actatcattt ttcttattat ttttaactca
    15541 tttacttctc caactaatgg aaccattatc tctggagaaa ctttaacacc tgctttgatt
    15601 gcttctatag caccttgtat tatagcttta gattgcatta cacatatttc aggataagtt
    15661 attgcaagtc tacaacctct gtgtccaagc attgggttaa actcatctaa atcaactatt
    15721 ctcttcttaa tatcacttac ttttattccc atagattttg aaagttcttc tatagtctca
    15781 tcatcatgag gcaaaaattc atgtagtggt ggatctagta atcttatatt aactggtttt
    15841 ccatccataa ctttaaataa ctctacaaaa tcttctcttt gcattggtaa tattttttca
    15901 agagcttttt ctctttgttc tactgtatta gataatatca tttctctaac tgctggcaat
    15961 ctagcttcat caaagaacat atgctcagtt ctacatagac ctataccttc tgcaccaaac
    16021 tctatagctg cacgagcatc tctaggatta tctgcattag ttctaaccat catacatttg
    16081 tgtttgtcta cccagttcat taatttttca aagtttccag tcaaagatac ttctgttttc
    16141 ttaacatctc ctaggtacac atttcctgta gaaccatcta tagaaatgta ttctccttct
    16201 tttataacta catcatcaag tgctcttatt tctttattaa actcatcaac ttttatttct
    16261 ccacatccag caacacaaca tttacccata cctctagcaa caactgctgc atgtgatgtc
    16321 attccacctc tagctgtaag gattccttct gctttaacca ttccttctat atcttctgga
    16381 gatgtctcta gtctaactaa aactactttt tctccttttt catttgcttt tactacatca
    16441 tcagcattga aataaacttt accacttgct gcacctggtg atgctggtaa accttttgct
    16501 ataaccttag cttcttttaa tgccttatct tcaaattttg gatgtaataa ttggtctaat
    16561 tgattaggct caattctcat gatagcttct ttttcatcaa ttattccagc ttcaactaaa
    16621 tcaaccgcta catttattgc tgctgctgct gttctcttac catttctagt ttgtagtata
    16681 tataatcttt cattttctat agtaaactct atatcttgca tatctttata atgaccttca
    16741 agtatatgag taatttttac aaattcatcg aatacagctg gcattacatc tgcaagagtt
    16801 gatatatttt gaggagttct tatacctgca acaacgtctt caccttgtgc attcattaaa
    16861 aattcaccaa ataatttatt ttctcctgta gctggatttc tagtaaatgc aacacctgta
    16921 ccactagttt ctcccatatt accaaatacc attgattgaa tatttactgc tgttcctaag
    16981 ttatgagcta tatcattcaa tttacggtat acgattgcac gaggattatt ccatgatcta
    17041 aatacagcct ctattgcaag cattaattgc tcttttgggt cttgtgggaa atcatttttt
    17101 atttcttttt tataaattgc tttaaattct tttactattt cttttaaatc atcagttgta
    17161 agttctgtat caactgtgta tccctttgct tcttttactc tgtctaatac attttcaaat
    17221 ttatattttg gaacttccat agcaacatca gaaaacattt gtatgaatct tctgtaacta
    17281 tcatatgcaa atctttcatt ttgtgtagcc tctgctaagc caacaacact attgtcatta
    17341 agacctaggt taagaattgt atccatcatt cctggcatag agaatactgc accagaacga
    17401 actgatacta ataatggatt cttattgcat cctaatgttt tatttaagtc tttttctaaa
    17461 gtagctaaat gctcttctat ttcttttatt atctctgctc ttatactttc attgttaaca
    17521 taataatcat tacatgcttc tgttgttata gtgaatccag gagggacagg taagcctatc
    17581 ttagtcattt ctgctaaatt ggcaccttta cctcctagta aagatttcat gtctttgctt
    17641 ccttcaccaa aactataaac atacttagtt tccattgtta aatcccccat taatcttttt
    17701 attttaattt atatcatttt tattaataga cattattaaa tttctatttc tttgtatatc
    17761 tttagaccat tttctttcat tatgtctaaa attatacctg ctgtttcttc tattgcctta
    17821 tttgaaacgt ttattactgg gcatccaatt cttttcatta tctcttctga ataatctaac
    17881 tcctgaagta ttctttctaa attagcgtag tttgctttac tggacaatcc taaagctttt
    17941 aatctttggg ttcttatctc attcagcttt tcaggtgaat tagtaagacc tattatcttt
    18001 ttagtatcta tctcaaacac ttctttagga attggtattt ctggaactaa tggaacatta
    18061 gctactttta tatttttatt agctaaatac atactcaaag gagttttaga tgttcttgat
    18121 attcccacta aaattatgtc tgcttgaaga acacctctag ggtcttttcc atcatcatat
    18181 ttcactgcaa attctatagc ttcaactctc ttaaagtaag attcatctaa ttttctaatt
    18241 ataccagctt ctcttttagg ttttcttcct gttctcttgc ttatttctct tagtatatct
    18301 gtcattaaat ctatacaact aagaccttct ctttcgcaat atctttcaac aatactgcat
    18361 aattcttcat caactaatgt atatacaatt aaagcgtttt cagcttttgc attctccaaa
    18421 gtttcaagta gtactccttt attaatcatg tatggaaatc tttttatttc ataagtttca
    18481 ttcacataaa actgagacat acatgccttt gcaacttgtt gtgcagtctc ccctactgaa
    18541 tctgatactg catatataat taggtttttc accagcatac ctccttaatt gtcaactatt
    18601 tctaagaata gcttagttat gttggttttt gatattctcc caacaactct catatgattt
    18661 ttgtcttctc ccttatattc aacaatcgga atcgaatcta cttcatgttc tattattttt
    18721 cttgaagcta ataaaacact tgctccttta gttaaagtga ctacatttgg agttcttgtc
    18781 attatcatac ctattggcat tttatttata tcagaacctc ctatagttgc ttttagcaag
    18841 tcttttctgg aaacaactcc acataactct tcattctcat ctataataac tatactccct
    18901 acatcagata aaaacatagt gactattaca tcatatatac tttcatcctt cttagctagt
    18961 acaggcatgc tcattatatc ctctactgtt ttttctttta cacttttacc aattaaattt
    19021 atttcactaa ctcctgaata aaaatatcca acttttggtc ttgcgtctaa tattcctgtc
    19081 attgttaata tagctaaatc agatctaagt gtagcacgag tcacattcaa gctggaggca
    19141 atactttcac tcgttattgg ttcattttcc ttaactatat ctataatttt tagttgtctt
    19201 tcattaagct gaataataat caccttcttt cattgtgcta tactttttat cttttcgcat
    19261 cttatatagt atattatata actatatata ccattctgta aacagatttt ttattgaata
    19321 cgatatattt tttactaagt tctctcgctt aaagatattt attatatatt aatgggggat
    19381 atttctttaa ttaatag
//
LOCUS       DAENCV010000066        19147 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAENCV010000066 DAENCV010000000
VERSION     DAENCV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.067"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            35..373
                     /locus_tag="KQD82_002386"
     CDS             35..373
                     /locus_tag="KQD82_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG4380853.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="KQD82_002387"
     CDS             complement(500..1789)
                     /locus_tag="KQD82_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG4380854.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="KQD82_002388"
     CDS             1869..2573
                     /locus_tag="KQD82_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBG4380855.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="KQD82_002389"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="KQD82_002389"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG4380856.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="KQD82_002390"
     CDS             3729..5141
                     /locus_tag="KQD82_002390"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG4380857.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="KQD82_002391"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="KQD82_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG4380858.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="KQD82_002392"
     CDS             7243..9981
                     /locus_tag="KQD82_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG4380859.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="KQD82_002393"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="KQD82_002393"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG4380860.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="KQD82_002394"
     CDS             11217..12578
                     /locus_tag="KQD82_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4380861.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="KQD82_002395"
     CDS             12666..14555
                     /locus_tag="KQD82_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4380862.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="KQD82_002396"
     CDS             14570..15025
                     /locus_tag="KQD82_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380863.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNSINEALGL"
     gene            15084..15401
                     /locus_tag="KQD82_002397"
     CDS             15084..15401
                     /locus_tag="KQD82_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG4380864.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="KQD82_002398"
     CDS             15426..16688
                     /locus_tag="KQD82_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG4380865.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="KQD82_002399"
     CDS             16793..17857
                     /locus_tag="KQD82_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4380866.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKALGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="KQD82_002400"
     CDS             17911..19089
                     /locus_tag="KQD82_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG4380867.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7700 a         1993 c         3352 g         6102 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatattata aattttttat
      481 tataatttct aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaacattat tttcagtcaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatt aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacaa taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aaccaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgactataga agagttggat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagatg acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggaact tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccacaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aggtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttaattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacattagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggagttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaattctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgatttg gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaggaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtgta attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctattgt tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggcaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat cttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagaggat attatcccag ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaatta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagaa
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatactatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggtagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt accacttgga acatagaaaa
    10561 agtctccctt ttttatgtta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca ttttttttag
    10741 catattcatc atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataattct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatatg aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagatatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tgggatttta tcaatggtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca agtatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctatt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatcctttg cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtgggtgt ttatccagtt agatatctcg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag caacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gcggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactc
    15001 aataaatgaa gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaactaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca tttataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcgg
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaagc tttaggtggt caaaaagaat ctttcgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat
    17401 agagtatgga gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag ggttttaaga ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagag acataattgg ttcatatgga tatgccaata attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttgtaccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaac acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac agggtgtaaa tgtaatttgt aatacactag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atccggaagg atatggacaa ttgaagaaat gacaagagta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctactga catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataaggatat aagggacaaa tttagaaata aattaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtattag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatggaag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gccttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat
    19141 atacata
//
LOCUS       DAENCV010000067        18835 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAENCV010000067 DAENCV010000000
VERSION     DAENCV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.068"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            130..684
                     /gene="tcdR"
                     /locus_tag="KQD82_002401"
     CDS             130..684
                     /gene="tcdR"
                     /locus_tag="KQD82_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG4380868.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1002..8102
                     /gene="tcdB"
                     /locus_tag="KQD82_002402"
                     /gene_synonym="toxB"
     CDS             1002..8102
                     /gene="tcdB"
                     /locus_tag="KQD82_002402"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG4380869.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8224..8724
                     /gene="tcdE"
                     /locus_tag="KQD82_002403"
     CDS             8224..8724
                     /gene="tcdE"
                     /locus_tag="KQD82_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG4380870.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9053..9235
                     /locus_tag="KQD82_002404"
     CDS             9053..9235
                     /locus_tag="KQD82_002404"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380871.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9452..17584
                     /gene="tcdA"
                     /locus_tag="KQD82_002405"
                     /gene_synonym="toxA"
     CDS             9452..17584
                     /gene="tcdA"
                     /locus_tag="KQD82_002405"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG4380872.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17919..18617)
                     /gene="tcdC"
                     /locus_tag="KQD82_002406"
     CDS             complement(17919..18617)
                     /gene="tcdC"
                     /locus_tag="KQD82_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG4380873.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPSCSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7449 a         2077 c         2913 g         6396 t
ORIGIN      
        1 tcataatgca tattttcata taaaatttaa tttatttgcc gattatataa ttataatgac
       61 tgatttaatt ccgatgttgt caaaattttc aaataaatca tcattataaa tataagagag
      121 gatgatttta tgcaaaagtc tttttatgaa ttaattgttt tagcaagaaa taactcagta
      181 gatgatttgc aagaaatttt atttatgttt aagccattag taaaaaaact tagtagagtt
      241 ttacattatg aagagggaga aacagattta ataatatttt ttattgaatt aataaaaaat
      301 attaaattaa gtagcttttc agaaaaaagc gatgctatta tagtcaaata tattcataaa
      361 tcattactga ataagacttt tgagttgtct agaagatatt ctaaaatgaa gtttaatttt
      421 gtagaatttg atgaaaatat cttaaatatg aaaaataatt atcaaagtaa gtctgttttt
      481 gaggaagata tttgtttttt cgaatatatt ttgaaagaat tatctggtat tcaaagaaaa
      541 gttatttttt ataaatattt aaaaggatat tctgatagag aaatatcagt gaaattaaaa
      601 atatctagac aagctgttaa taaggctaaa aatagagcat ttaaaaaaat aaaaaaagac
      661 tatgaaaatt attttaactt gtaattaatg agcttaaaga aatatttaca atagaaatca
      721 aattttagaa ttaactttat tgtaaaatca ataacttaat ctaagaatat cttaattttt
      781 atattttata tagaacaaag tttacatatt tatttcagac aacgtcttta ttcaatcgaa
      841 gagcaaatta atcaactgag tgtcttaaat ttaaaatgtt aggaagtgaa tgtatatgaa
      901 aacctaagta gatattagta tattttataa atagaaagga ggatatataa aagagtttta
      961 gcatttagat gtaaaaatat tatagtaaag gagaaaattt tatgagttta gttaatagaa
     1021 aacagttaga aaaaatggca aatgtaagat ttcgtactca agaagatgaa tatgttgcaa
     1081 tattggatgc tttagaagaa tatcataata tgtcagagaa tactgtagtc gaaaaatatt
     1141 taaaattaaa agatataaat agtttaacag atatttatat agatacatat aaaaaatctg
     1201 gtagaaataa agccttaaaa aaatttaagg aatatctagt tacagaagta ttagagctaa
     1261 agaataataa tttaactcca gttgagaaaa atttacattt tgtttggatt ggaggtcaaa
     1321 taaatgacac tgctattaat tatataaatc aatggaaaga tgtaaatagt gattataatg
     1381 ttaatgtttt ttatgatagt aatgcatttt tgataaacac attgaaaaaa actgtagtag
     1441 aatcagcaat aaatgataca cttgaatcat ttagagaaaa cttaaatgac cctagatttg
     1501 actataataa attcttcaga aaacgtatgg aaataattta tgataaacag aaaaatttca
     1561 taaactacta taaagctcaa agagaagaaa atcctgaact tataattgat gatattgtaa
     1621 agacatatct ttcaaatgag tattcaaagg agatagatga acttaatacc tatattgaag
     1681 aatccttaaa taaaattaca cagaatagtg gaaatgatgt tagaaacttt gaagaattta
     1741 aaaatggaga gtcattcaac ttatatgaac aagagttggt agaaaggtgg aatttagctg
     1801 ctgcttctga catattaaga atatctgcat taaaagaaat tggtggtatg tatttagatg
     1861 ttgatatgtt accaggaata caaccagact tatttgagtc tatagagaaa cctagttcag
     1921 taacagtgga tttttgggaa atgacaaagt tagaagctat aatgaaatac aaagaatata
     1981 taccagaata tacctcagaa cattttgaca tgttagacga agaagttcaa agtagttttg
     2041 aatctgttct agcttctaag tcagataaat cagaaatatt ctcatcactt ggtgatatgg
     2101 aggcatcacc actagaagtt aaaattgcat ttaatagtaa gggtattata aatcaagggc
     2161 taatttctgt gaaagactca tattgtagca atttaatagt aaaacaaatc gagaatagat
     2221 ataaaatatt gaataatagt ttaaatccag ctattagcga ggataatgat tttaatacta
     2281 caacgaatac ctttattgat agtataatgg ctgaagctaa tgcagataat ggtagattta
     2341 tgatggaact aggaaagtat ttaagagttg gtttcttccc agatgttaaa actactatta
     2401 acttaagtgg ccctgaagca tatgcggcag cttatcaaga tttattaatg tttaaagaag
     2461 gcagtatgaa tatccatttg atagaagctg atttaagaaa ctttgaaatc tctaaaacta
     2521 atatttctca atcaactgaa caagaaatgg ctagcttatg gtcatttgac gatgcaagag
     2581 ctaaagctca atttgaagaa tataaaagga attattttga aggttctctt ggtgaagatg
     2641 ataatcttga tttttctcaa aatatagtag ttgacaagga gtatctttta gaaaaaatat
     2701 cttcattagc aagaagttca gagagaggat atatacacta tattgttcag ttacaaggag
     2761 ataaaattag ttatgaagca gcatgtaact tatttgcaaa gactccttat gatagtgtac
     2821 tatttcagaa aaatatagaa gattcagaaa ttgcatatta ttataatcct ggagatggtg
     2881 aaatacaaga aatagacaag tataaaattc caagtataat ttctgataga cctaagatta
     2941 aattaacatt tattggtcat ggtaaagatg aatttaatac tgatatattt gcaggttttg
     3001 atgtagattc attatccaca gaaatagaag cagcaataga tttagctaaa gaggatattt
     3061 ctcctaagtc aatagaaata aatttattag gatgtaatat gtttagctac tctatcaacg
     3121 tagaggagac ttatcctgga aaattattac ttaaagttaa agataaaata tcagaattaa
     3181 tgccatctat aagtcaagac tctattatag taagtgcaaa tcaatatgaa gttagaataa
     3241 atagtgaagg aagaagagaa ttattggatc attctggtga atggataaat aaagaagaaa
     3301 gtattataaa ggatatttca tcaaaagaat atatatcatt taatcctaaa gaaaataaaa
     3361 ttacagtaaa atctaaaaat ttacctgagc tatctacatt attacaagaa attagaaata
     3421 attctaattc aagtgatatt gaactagaag aaaaagtaat gttaacagaa tgtgagataa
     3481 atgttatttc aaatatagat acgcaaattg ttgaggaaag gattgaagaa gctaagaatt
     3541 taacttctga ctctattaat tatataaaag atgaatttaa actaatagaa tctatttctg
     3601 atgcactatg tgacttaaaa caacagaatg aattagaaga ttctcatttt atatcttttg
     3661 aggacatatc agagactgat gagggattta gtataagatt tattaataaa gaaactggag
     3721 aatctatatt tgtagaaact gaaaaaacaa tattctctga atatgctaat catataactg
     3781 aagagatttc taagataaaa ggtactatat ttgatactgt aaatggtaag ttagtaaaaa
     3841 aagtaaattt agatactaca cacgaagtaa atactttaaa tgctgcattt tttatacaat
     3901 cattaataga atataatagt tctaaagaat ctcttagtaa tttaagtgta gcaatgaaag
     3961 tccaagttta cgctcaatta tttagtactg gtttaaatac tattacagat gcagccaaag
     4021 ttgttgaatt agtatcaact gcattagatg aaactataga cttacttcct acattatctg
     4081 aaggattacc tataattgca actattatag atggtgtaag tttaggtgca gcaatcaaag
     4141 agctaagtga aacgagtgac ccattattaa gacaagaaat agaagctaag ataggtataa
     4201 tggcagtaaa tttaacaaca gctacaactg caatcattac ttcatctttg ggggtagcta
     4261 gtggatttag tatactttta gttcctttag caggaatttc agcaggtata ccaagcttag
     4321 taaacaatga acttgtactt cgagataagg caacaaaggt tgtagattat tttaaacatg
     4381 tttcattagt tgaaactgaa ggagtattta ctttattaga tgataaaata atgatgccac
     4441 aagatgattt agtgatatca gaaatagatt ttaataataa ttcaatagtt ttaggtaaat
     4501 gtgaaatctg gagaatggaa ggtggttcag gtcatactgt aactgatgat atagatcact
     4561 tcttttcagc accatcaata acatatagag agccacactt atctatatat gacgtattgg
     4621 aagtacaaaa agaagaactt gatttgtcaa aagatttaat ggtattacct aatgctccaa
     4681 atagagtatt tgcttgggaa acaggatgga caccaggttt aagaagctta gaaaatgatg
     4741 gcacaaaact gttagaccgt ataagagata actatgaagg tgagttttat tggagatatt
     4801 ttgcttttat agctgatgct ttaataacaa cattaaaacc aagatatgaa gatactaata
     4861 taagaataaa tttagatagt aatactagaa gttttatagt tccaataata actacagaat
     4921 atataagaga aaaattatca tattctttct atggttcagg aggaacttat gcattgtctc
     4981 tttctcaata taatatgggt ataaatatag aattaagtga aagtgatgtt tggattatag
     5041 atgttgataa tgttgtgaga gatgtaacta tagaatctga taaaattaaa aaaggtgatt
     5101 taatagaagg tattttatct acactaagta ttgaagagaa taaaattatc ttaaatagcc
     5161 atgagattaa tttttctggt gaggtaaatg gaagtaatgg atttgtttct ttaacatttt
     5221 caattttaga aggaataaat gcaattatag aagttgattt attatctaaa tcatataaat
     5281 tacttatttc tggcgaatta aaaatattga tgttaaattc aaatcatatt caacagaaaa
     5341 tagattatat aggattcaat agcgaattac agaaaaatat accatatagc tttgtagata
     5401 gtgaaggaaa agagaatggt tttattaatg gttcaacaaa agaaggttta tttgtatctg
     5461 aattacctga tgtagttctt ataagtaagg tttatatgga tgatagtaag ccttcatttg
     5521 gatattatag taataatttg aaagatgtca aagttataac taaagataat gttaatatat
     5581 taacaggtta ttatcttaag gatgatataa aaatctctct ttctttgact ctacaagatg
     5641 aaaaaactat aaagttaaat agtgtgcatt tagatgaaag tggagtagct gagattttga
     5701 agttcatgaa tagaaaaggt aatacaaata cttcagattc tttaatgagc tttttagaaa
     5761 gtatgaatat aaaaagtatt ttcgttaatt tcttacaatc taatattaag tttatattag
     5821 atgctaattt tataataagt ggtactactt ctattggcca atttgagttt atttgtgatg
     5881 aaaatgataa tatacaacca tatttcatta agtttaatac actagaaact aattatactt
     5941 tatatgtagg aaatagacaa aatatgatag tggaaccaaa ttatgattta gatgattctg
     6001 gagatatatc ttcaactgtt atcaatttct ctcaaaagta tctttatgga atagacagtt
     6061 gtgttaataa agttgtaatt tcaccaaata tttatacaga tgaaataaat ataacgcctg
     6121 tatatgaaac aaataatact tatccagaag ttattgtatt agatgcaaat tatataaatg
     6181 aaaaaataaa tgttaatatc aatgatctat ctatacgata tgtatggagt aatgatggta
     6241 atgattttat tcttatgtca actagtgaag aaaataaggt gtcacaagtt aaaataagat
     6301 tcgttaatgt ttttaaagat aagactttgg caaataagct atcttttaac tttagtgata
     6361 aacaagatgt acctgtaagt gaaataatct tatcatttac accttcatat tatgaggatg
     6421 gattgattgg ctatgatttg ggtctagttt ctttatataa tgagaaattt tatattaata
     6481 actttggaat gatggtatct ggattaatat atattaatga ttcattatat tattttaaac
     6541 caccagtaaa taatttgata actggatttg tgactgtagg cgatgataaa tactacttta
     6601 atccaattaa tggtggagct gcttcaattg gagagacaat aattgatgac aaaaattatt
     6661 atttcaacca aagtggagtg ttacaaacag gtgtatttag tacagaagat ggatttaaat
     6721 attttgcccc agctaataca cttgatgaaa acctagaagg agaagcaatt gattttactg
     6781 gaaaattaat tattgacgaa aatatttatt attttgatga taattataga ggagctgtag
     6841 aatggaaaga attagatggt gaaatgcact attttagccc agaaacaggt aaagctttta
     6901 aaggtctaaa tcaaataggt gattataaat actatttcaa ttctgatgga gttatgcaaa
     6961 aaggatttgt tagtataaat gataataaac actattttga tgattctggt gttatgaaag
     7021 taggttacac tgaaatagat ggcaagcatt tctactttgc tgaaaacgga gaaatgcaaa
     7081 taggagtatt taatacagaa gatggattta aatattttgc tcatcataat gaagatttag
     7141 gaaatgaaga aggtgaagaa atctcatatt ctggtatatt aaatttcaat aataaaattt
     7201 actattttga tgattcattt acagctgtag ttggatggaa agatttagag gatggttcaa
     7261 agtattattt tgatgaagat acagcagaag catatatagg tttgtcatta ataaatgatg
     7321 gtcaatatta ttttaatgat gatggaatta tgcaagttgg atttgtcact ataaatgata
     7381 aagtcttcta cttctctgac tctggaatta tagaatctgg agtacaaaac atagatgaca
     7441 attatttcta tatagatgat aatggtatag ttcaaattgg tgtatttgat acttcagatg
     7501 gatataaata ttttgcacct gctaatactg taaatgataa tatttacgga caagcagttg
     7561 aatatagtgg tttagttaga gttggggaag atgtatatta ttttggagaa acatatacaa
     7621 ttgagactgg atggatatat gatatggaaa atgaaagtga taaatattat ttcaatccag
     7681 aaactaaaaa agcatgcaaa ggtattaatt taattgatga tataaaatat tattttgatg
     7741 agaagggcat aatgagaacg ggtcttatat catttgaaaa taataattat tactttaatg
     7801 agaatggtga aatgcaattt ggttatataa atatagaaga taagatgttc tattttggtg
     7861 aagatggtgt catgcagatt ggagtattta atacaccaga tggatttaaa tactttgcac
     7921 atcaaaatac tttggatgag aattttgagg gagaatcaat aaactatact ggttggttag
     7981 atttagatga aaagagatat tattttacag atgaatatat tgcagcaact ggttcagtta
     8041 ttattgatgg tgaggagtat tattttgatc ctgatacagc tcaattagtg attagtgaat
     8101 agataaaaat atgttaaata tatcctctta tacttaaata tataaaaata aacaaaatga
     8161 tacactacat aaagtgttct atctaatatg aagatttacc aataaaaagg tggactatga
     8221 tgaatgcaca gtagttcacc tttttatatt tctaatggta acaaaatatt tttttatata
     8281 aacctaggag gcgttatgaa tatgacaata tcttttttat cagagcatat atttataaag
     8341 ttagtaattt taactatatc atttgataca ttattaggat gtttaagtgc aataaaaagt
     8401 cgtaaattta attctagttt tggaatagat ggaggaatca gaaaagtagc aatgatagca
     8461 tgtatatttt ttttatcagt agttgacatt cttacaaagt ttaacttttt atttatgtta
     8521 ccacaagatt gtatcaattt tttaagacta aaacatcttg gaatatctga atttttctct
     8581 attttattta ttttatatga aagtgtaagt atattaaaaa atatgtgctt atgtggatta
     8641 ccagtaccta agagattaaa ggaaaaaata gcaattttac tagatgcaat gacagatgaa
     8701 atgaatgcta aggatgaaaa gtaagtaatg gtagatataa taaagatatt aacaaataaa
     8761 aagtgttatc caaataagaa tagctgaaag ttatcataat tcatgaaact aataatgaaa
     8821 acgagggagc agatgccaag agacacacaa gtattaaata catataattt cgaagcaagt
     8881 gttcattact atatagatga caaggtagta tatcaaacat tggttcacaa agatggtgca
     8941 tggtcagttg gtaaaatcta ttaagctaca ttagttacag atatcacaaa ctataatagt
     9001 taaacataga aatatgtgta aattgtgatg gaaattattc aaaaacacaa aaatacgtga
     9061 tgaaggacaa aatgatatag aaaataagta tcaaacctta ataaatgatt taattgatag
     9121 tttaaaagtt ataggaaaaa tatataaaga aataaaaaca ttaaaaaaat ataagatatg
     9181 tttacaaatt actatcagac aatctcctta tctaatagaa gagtcaatta actaattgag
     9241 tatctttaaa ttgaaatgtt aggaagtgat ttaaatatga aaacttaaat tataaaaaat
     9301 caatattaat ttatttttaa aaaatagaaa ggagtgtata agatttattt tcaaagttta
     9361 aaaacaagaa aatcaattta aatttcagaa ggaataaatg tggttataga agtggattta
     9421 ttatcaaaaa taataatact aggaggtttt tatgtcttta atatctaaag aagagttaat
     9481 aaaactcgca tatagcatta gaccaagaga aaatgagtat aaaactatac taactaattt
     9541 agacgaatat aataagttaa ctacaaacaa taatgaaaat aaatatttac aattaaaaaa
     9601 actaaatgaa tcaattgatg tttttatgaa taaatataaa acttcaagca gaaatagagc
     9661 actctctaat ctaaaaaaag atatattaaa agaagtaatt cttattaaaa attccaatac
     9721 aagccctgta gaaaaaaatt tacattttgt atggataggt ggagaagtca gtgatattgc
     9781 tcttgaatac ataaaacaat gggctgatat taatgcagaa tataatatta aactgtggta
     9841 tgatagtgaa gcattcttag taaatacact aaaaaaggct atagttgaat cttctaccac
     9901 tgaagcatta cagctactag aggaagagat tcaaaatcct caatttgata atatgaaatt
     9961 ttacaaaaaa aggatggaat ttatatatga tagacaaaaa aggtttataa attattataa
    10021 atctcaaatc aataaaccta cagtacctac aatagatgat attataaagt ctcatctagt
    10081 atctgaatat aatagagatg aaactgtatt agaatcatat agaacaaatt ctttgagaaa
    10141 aataaatagt aatcatggga tagatatcag ggctaatagt ttgtttacag aacaagagtt
    10201 attaaatatt tatagtcagg agttgttaaa tcgtggaaat ttagctgcag catctgacat
    10261 agtaagatta ttagccctaa aaaattttgg cggagtatat ttagatgttg atatgcttcc
    10321 aggtattcac tctgatttat ttaaaacaat atctagacct agctctattg gactagaccg
    10381 ttgggaaatg ataaaattag aggctattat gaagtataaa aaatatataa ataattatac
    10441 atcagaaaac tttgataaac ttgatcaaca attaaaagat aattttaaac tcattataga
    10501 aagtaaaagt gaaaaatctg agatattttc taaattagaa aatttaaatg tatctgatct
    10561 tgaaattaaa atagctttcg ctttaggcag tgttataaat caagccttga tatcaaaaca
    10621 aggttcatat cttactaacc tagtaataga acaagtaaaa aatagatatc aatttttaaa
    10681 ccaacacctt aacccagcca tagagtctga taataacttc acagatacta ctaaaatttt
    10741 tcatgattca ttatttaatt cagctaccgc agaaaactct atgtttttaa caaaaatagc
    10801 accatactta caagtaggtt ttatgccaga agctcgctcc acaataagtt taagtggtcc
    10861 aggagcttat gcgtcagctt actatgattt cataaattta caagaaaata ctatagaaaa
    10921 aactttaaaa gcatcagatt taatagaatt taaattccca gaaaataatc tatctcaatt
    10981 gacagaacaa gaaataaata gtctatggag ctttgatcaa gcaagtgcaa aatatcaatt
    11041 tgagaaatat gtaagagatt atactggtgg atctctttct gaagacaatg gggtagactt
    11101 taataaaaat actgccctcg acaaaaacta tttattaaat aataaaattc catcaaacaa
    11161 tgtagaagaa gctggaagta aaaattatgt tcattatatc atacagttac aaggagatga
    11221 tataagttat gaagcaacat gcaatttatt ttctaaaaat cctaaaaata gtattattat
    11281 acaacgaaat atgaatgaaa gtgcaaaaag ctacttttta agtgatgatg gagaatctat
    11341 tttagaatta aataaatata ggatacctga aagattaaaa aataaggaaa aagtaaaagt
    11401 aacctttatt ggacatggta aagatgaatt caacacaagc gaatttgcta gattaagtgt
    11461 agattcactt tccaatgaga taagttcatt tttagatacc ataaaattag atatatcacc
    11521 taaaaatgta gaagtaaact tacttggatg taatatgttt agttatgatt ttaatgttga
    11581 agaaacttat cctgggaagt tgctattaag tattatggac aaaattactt ccactttacc
    11641 tgatgtaaat aaaaattcta ttactatagg agcaaatcaa tatgaagtaa gaattaatag
    11701 tgagggaaga aaagaacttc tggctcactc aggtaaatgg ataaataaag aagaagctat
    11761 tatgagcgat ttatctagta aagaatacat tttttttgat tctatagata ataagctaaa
    11821 agcaaagtcc aagaatattc caggattagc atcaatatca gaagatataa aaacattatt
    11881 acttgatgca agtgttagtc ctgatacaaa atttatttta aataatctta agcttaatat
    11941 tgaatcttct attggtgatt acatttatta tgaaaaatta gagcctgtta aaaatataat
    12001 tcacaattct atagatgatt taatagatga gttcaatcta cttgaaaatg tatctgatga
    12061 attatatgaa ttaaaaaaat taaataatct agatgagaag tatttaatat cttttgaaga
    12121 tatctcaaaa aataattcaa cttactctgt aagatttatt aacaaaagta atggtgagtc
    12181 agtttatgta gaaacagaaa aagaaatttt ttcaaaatat agcgaacata ttacaaaaga
    12241 aataagtact ataaagaata gtataattac agatgttaat ggtaatttat tggataatat
    12301 acagttagat catacttctc aagttaatac attaaacgca gcattcttta ttcaatcatt
    12361 aatagattat agtagcaata aagatgtact gaatgattta agtacctcag ttaaggttca
    12421 actttatgct caactattta gtacaggttt aaatactata tatgactcta tccaattagt
    12481 aaatttaata tcaaatgcag taaatgatac tataaatgta ctacctacaa taacagaggg
    12541 gatacctatt gtatctacta tattagacgg aataaactta ggtgcagcaa ttaaggaatt
    12601 actagacgaa catgacccat tactaaaaaa agaattagaa gctaaggtgg gtgttttagc
    12661 aataaatatg tcattatcta tagctgcaac tgtagcttca attgttggaa taggtgctga
    12721 agttactatt ttcttattac ctatagctgg tatatctgca ggaatacctt cattagttaa
    12781 taatgaatta atattgcatg ataaggcaac ttcagtggta aactatttta atcatttgtc
    12841 tgaatctaaa aaatatggcc ctcttaagac agaagatgat aaaattttag ttcctattga
    12901 tgatttagta atatcagaaa tagattttaa taataattcg ataaaactag gaacatgtaa
    12961 tatattagca atggaggggg gatcaggaca cacagtgact ggtaatatag atcacttttt
    13021 ctcatctcca tctataagtt ctcatattcc ttcattatca atttattctg caataggtat
    13081 agaaacagaa aatctagatt tttcaaaaaa aataatgatg ttacctaatg ctccttcaag
    13141 agtgttttgg tgggaaactg gagcagttcc aggtttaaga tcattggaaa atgacggaac
    13201 tagattactt gattcaataa gagatttata cccaggtaaa ttttactgga gattctatgc
    13261 ttttttcgat tatgcaataa ctacattaaa accagtttat gaagacacta atattaaaat
    13321 taaactagat aaagatacta gaaacttcat aatgccaact ataactacta acgaaattag
    13381 aaacaaatta tcttattcat ttgatggagc aggaggaact tactctttat tattatcttc
    13441 atatccaata tcaacgaata taaatttatc taaagatgat ttatggatat ttaatattga
    13501 taatgaagta agagaaatat ctatagaaaa tggtactatt aaaaaaggaa agttaataaa
    13561 agatgtttta agtaaaattg atataaataa aaataaactt attataggca atcaaacaat
    13621 agatttttca ggcgatatag ataataaaga tagatatata ttcttgactt gtgagttaga
    13681 tgataaaatt agtttaataa tagaaataaa tcttgttgca aaatcttata gtttgttatt
    13741 gtctggggat aaaaattatt tgatatccaa tttatctaat actattgaga aaatcaatac
    13801 tttaggccta gatagtaaaa atatagcgta caattacact gatgaatcta ataataaata
    13861 ttttggagct atatctaaaa caagtcaaaa aagcataata cattataaaa aagacagtaa
    13921 aaatatatta gaattttata atgacagtac attagaattt aacagtaaag attttattgc
    13981 tgaagatata aatgtattta tgaaagatga tattaatact ataacaggaa aatactatgt
    14041 tgataataat actgataaaa gtatagattt ctctatttct ttagttagta aaaatcaagt
    14101 aaaagtaaat ggattatatt taaatgaatc cgtatactca tcttaccttg attttgtgaa
    14161 aaattcagat ggacaccata atacttctaa ttttatgaat ttatttttgg acaatataag
    14221 tttctggaaa ttgtttgggt ttgaaaatat aaattttgta atcgataaat actttaccct
    14281 tgttggtaaa actaatcttg gatatgtaga atttatttgt gacaataata aaaatataga
    14341 tatatatttt ggtgaatgga aaacatcgtc atctaaaagc actatattta gcggaaatgg
    14401 tagaaatgtt gtagtagagc ctatatataa tcctgatacg ggtgaagata tatctacttc
    14461 actagatttt tcctatgaac ctctctatgg aatagataga tatatcaata aagtattgat
    14521 agcacctgat ttatatacaa gtttaataaa tattaatacc aattattatt caaatgagta
    14581 ctaccctgag attatagttc ttaacccaaa tacattccac aaaaaagtaa atataaattt
    14641 agatagttct tcttttgagt ataaatggtc tacagaagga agtgacttta ttttagttag
    14701 atacttagaa gaaagtaata aaaaaatatt acaaaaaata agaatcaaag gtatcttatc
    14761 taatactcaa tcatttaata aaatgagtat agattttaaa gatattaaaa aactatcatt
    14821 aggatatata atgagtaatt ttaaatcatt taattctgaa aatgaattag atagagatca
    14881 tttaggattt aaaataatag ataataaaac ttattactat gatgaagata gtaaattagt
    14941 taaaggatta atcaatataa ataattcatt attctatttt gatcctatag aatttaactt
    15001 agtaactgga tggcaaacta tcaatggtaa aaaatattat tttgatataa atactggagc
    15061 agctttaact agttataaaa ttattaatgg taaacacttt tattttaata atgatggtgt
    15121 gatgcagttg ggagtattta aaggacctga tggatttgaa tattttgcac ctgccaatac
    15181 tcaaaataat aacatagaag gtcaggctat agtttatcaa agtaaattct taactttgaa
    15241 tggcaaaaaa tattattttg ataatgactc aaaagcagtc actggatgga gaattattaa
    15301 caatgagaaa tattacttta atcctaataa tgctattgct gcagtcggat tgcaagtaat
    15361 tgacaataat aagtattatt tcaatcctga cactgctatc atctcaaaag gttggcagac
    15421 tgttaatggt agtagatact actttgatac tgataccgct attgccttta atggttataa
    15481 aactattgat ggtaaacact tttattttga tagtgattgt gtagtgaaaa taggtgtgtt
    15541 tagtacctct aatggatttg aatattttgc acctgctaat acttataata ataacataga
    15601 aggtcaggct atagtttatc aaagtaaatt cttaactttg aatggtaaaa aatattactt
    15661 tgataataac tcaaaagcag ttaccggatg gcaaactatt gatagtaaaa aatattactt
    15721 taatactaac actgctgaag cagctactgg atggcaaact attgatggta aaaaatatta
    15781 ctttaatact aacactgctg aagcagctac tggatggcaa actattgatg gtaaaaaata
    15841 ttactttaat actaacactg ctatagcttc aactggttat acaattatta atggtaaaca
    15901 tttttatttt aatactgatg gtattatgca gataggagtg tttaaaggac ctaatggatt
    15961 tgaatatttt gcacctgcta atacggatgc taacaacata gaaggtcaag ctatacttta
    16021 ccaaaatgaa ttcttaactt tgaatggtaa aaaatattac tttggtagtg actcaaaagc
    16081 agttactgga tggagaatta ttaacaataa gaaatattac tttaatccta ataatgctat
    16141 tgctgcaatt catctatgca ctataaataa tgacaagtat tactttagtt atgatggaat
    16201 tcttcaaaat ggatatatta ctattgaaag aaataatttc tattttgatg ctaataatga
    16261 atctaaaatg gtaacaggag tatttaaagg acctaatgga tttgagtatt ttgcacctgc
    16321 taatactcac aataataaca tagaaggtca ggctatagtt taccagaaca aattcttaac
    16381 tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagttactg gatggcaaac
    16441 cattgatggt aaaaaatatt actttaatct taacactgct gaagcagcta ctggatggca
    16501 aactattgat ggtaaaaaat attactttaa tcttaacact gctgaagcag ctactggatg
    16561 gcaaactatt gatggtaaaa aatattactt taatactaac actttcatag cctcaactgg
    16621 ttatacaagt attaatggta aacattttta ttttaatact gatggtatta tgcagatagg
    16681 agtgtttaaa ggacctaatg gatttgaata ctttgcacct gctaatactc ataataataa
    16741 catagaaggt caagctatac tttaccaaaa taaattctta actttgaatg gtaaaaaata
    16801 ttactttggt agtgactcaa aagcagttac cggattgcga actattgatg gtaaaaaata
    16861 ttactttaat actaacactg ctgttgcagt tactggatgg caaactatta atggtaaaaa
    16921 atactacttt aatactaaca cttctatagc ttcaactggt tatacaatta ttagtggtaa
    16981 acatttttat tttaatactg atggtattat gcagatagga gtgtttaaag gacctgatgg
    17041 atttgaatac tttgcacctg ctaatacaga tgctaacaat atagaaggtc aagctatacg
    17101 ttatcaaaat agattcctat atttacatga caatatatat tattttggta ataattcaaa
    17161 agcggctact ggttgggtaa ctattgatgg taatagatat tacttcgagc ctaatacagc
    17221 tatgggtgcg aatggttata aaactattga taataaaaat ttttacttta gaaatggttt
    17281 acctcagata ggagtgttta aagggtctaa tggatttgaa tactttgcac ctgctaatac
    17341 ggatgctaac aatatagaag gtcaagctat acgttatcaa aatagattcc tacatttact
    17401 tggaaaaata tattactttg gtaataattc aaaagcagtt actggatggc aaactattaa
    17461 tggtaaagta tattacttta tgcctgatac tgctatggct gcagctggtg gacttttcga
    17521 gattgatggt gttatatatt tctttggtgt tgatggagta aaagcccctg ggatatatgg
    17581 ctaaaatata tgtttgataa aaaattattc ctgtgctact aagaaattat ttttatataa
    17641 taaatattga gatttaatta aaagtcatgt gttattgtaa tacatgactt ttagttaaaa
    17701 tttttctatc atttaataat ctattattct tgactatttt ataataaaat tcatatatgg
    17761 aaatattaat actaaataat taatagttga taaaaaatag ataatatgct aaaagcaaaa
    17821 actaatttag agccttgtaa ctgtttattt gcaattataa aaacatcttt aaacatattg
    17881 actataatat aaaatattaa ctataataca aaacaatatt aattaatttt ctctacagct
    17941 atccctggta tagttatatt tccacccata gttgattcat aagttatagt tccagcactt
    18001 atacctctta tagttatgta atcatcctct aatactcttg aaggagttat tgatttttta
    18061 taactacata gtacaacctt atcataattt ccagacacag ctaatcttat ttgcacctca
    18121 tcaccatctt caataacttg aataacctta ccttcaaatt ttaccttttt gtacttataa
    18181 tcatcaggtg ttctagctaa ttggtcataa gtaataccag tatcatatcc tttcttctcc
    18241 tcttcttctt ttttgcgttg ttcttcagcc tttttagctt cttcagcttt tttagcttct
    18301 tcagctttac gttgattttc agcttcaata gctttctttt cgtcgtcttt cattttgaac
    18361 catggttcag catcagacaa tttgctattt aaagtttcta tttctgtttt ttgagaatct
    18421 ataactgatg tttggttaga aatgacctcc tcatggtctt cagaacaaga tggtgaggat
    18481 atattgccaa aaaacacgcc taaaataaat gccagtagag caataccctt tgtgctctta
    18541 aagaatttaa ttattttctt agagcttcct tttccttcat tactaaattc gttaccctca
    18601 ttttttttag aaaacataat acaatctccc ttttattaga tatattttat tttaaaagtt
    18661 aaaaacttaa aataactagt tgactaaaaa ttatatattc atattttcct aaaatatatt
    18721 tcccctaagc ataattttac aaaataaagt aagaagattc aatatatttt gaaaaaataa
    18781 gataaaaatg tcagattata atataatttt cttgaaggtg aattttatat attaa
//
LOCUS       DAENCV010000068        18834 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAENCV010000068 DAENCV010000000
VERSION     DAENCV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.069"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(270..662)
                     /locus_tag="KQD82_002407"
     CDS             complement(270..662)
                     /locus_tag="KQD82_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4380874.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1229..1591
                     /locus_tag="KQD82_002408"
     CDS             1229..1591
                     /locus_tag="KQD82_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380875.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1575..2291
                     /locus_tag="KQD82_002409"
     CDS             1575..2291
                     /locus_tag="KQD82_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380876.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYLLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            2311..2730
                     /locus_tag="KQD82_002410"
     CDS             2311..2730
                     /locus_tag="KQD82_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380877.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2720..3244
                     /locus_tag="KQD82_002411"
     CDS             2720..3244
                     /locus_tag="KQD82_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380878.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIIGNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3556..4113
                     /gene="efp"
                     /locus_tag="KQD82_002412"
     CDS             3556..4113
                     /gene="efp"
                     /locus_tag="KQD82_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG4380879.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4205..4660
                     /locus_tag="KQD82_002413"
     CDS             4205..4660
                     /locus_tag="KQD82_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG4380880.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4823..5533
                     /locus_tag="KQD82_002414"
     CDS             4823..5533
                     /locus_tag="KQD82_002414"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG4380881.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5587..6657
                     /locus_tag="KQD82_002415"
     CDS             5587..6657
                     /locus_tag="KQD82_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4380882.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     IFQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6685..10239
                     /gene="smc"
                     /locus_tag="KQD82_002416"
     CDS             6685..10239
                     /gene="smc"
                     /locus_tag="KQD82_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG4380883.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRNVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10266..11546
                     /gene="ftsY"
                     /locus_tag="KQD82_002417"
     CDS             10266..11546
                     /gene="ftsY"
                     /locus_tag="KQD82_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG4380884.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     FEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEIV
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11649..12017
                     /locus_tag="KQD82_002418"
     CDS             11649..12017
                     /locus_tag="KQD82_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG4380885.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNKD
                     CANLIPKLENIEDVCREMIK"
     gene            12014..13366
                     /gene="ffh"
                     /locus_tag="KQD82_002419"
     CDS             12014..13366
                     /gene="ffh"
                     /locus_tag="KQD82_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG4380886.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13404..13676
                     /gene="rpsP"
                     /locus_tag="KQD82_002420"
     CDS             13404..13676
                     /gene="rpsP"
                     /locus_tag="KQD82_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG4380887.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13741..13968
                     /locus_tag="KQD82_002421"
     CDS             13741..13968
                     /locus_tag="KQD82_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG4380888.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14076..14591
                     /gene="rimM"
                     /locus_tag="KQD82_002422"
     CDS             14076..14591
                     /gene="rimM"
                     /locus_tag="KQD82_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG4380889.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14600..15295
                     /gene="trmD"
                     /locus_tag="KQD82_002423"
     CDS             14600..15295
                     /gene="trmD"
                     /locus_tag="KQD82_002423"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG4380890.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15480..15830
                     /gene="rplS"
                     /locus_tag="KQD82_002424"
     CDS             15480..15830
                     /gene="rplS"
                     /locus_tag="KQD82_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG4380891.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16151..17047
                     /gene="ylqF"
                     /locus_tag="KQD82_002425"
     CDS             16151..17047
                     /gene="ylqF"
                     /locus_tag="KQD82_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG4380892.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17436..18728
                     /gene="brnQ"
                     /locus_tag="KQD82_002426"
     CDS             17436..18728
                     /gene="brnQ"
                     /locus_tag="KQD82_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG4380893.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7956 a         1756 c         3472 g         5650 t
ORIGIN      
        1 atactattta aatagatatt aaaaaattga taccaagtat ttgtttgcta tatgaataac
       61 aatttattta ggaactttac tttaacactg tataataata aagtaattgc taaaaacgtt
      121 tgatgatttg gatgtatttt tagaaattag aaaaatatat acttactgac taatgataat
      181 cttatatgta ttttagtttg agtgatttaa gaaaagtgtt ttgaaaggga attattttat
      241 agagtaaata aatatataat agtagaaaat tacatataaa gagtagtgtt tatttcatta
      301 ccttctttat ctgttccgat agggtcttgt agagatactt gagttttatt ttttttgtta
      361 gatctaatat tcattaaaat ctcattttca atacatctag aagcatatgt tgctaatcga
      421 gttcctttat ctatgttata agtttctata gctttaatta atcctattgt acctatggat
      481 attaaatcat cctgatcttc cgttgaatta ttatatttct ttgctatata agctactaat
      541 ctcatgtttc tttcaattaa tgtatctttt gcacttttat cgttttctat tttaaattta
      601 gttaaatact caatctcttc ttcaggggtt aagggttttt caaaagattt aagagctgcc
      661 aataggtcac ccccttttca acggtcactt taaattatat gaaggagact tatccaagtt
      721 gactgttatc taataaaata atatgcatat ttttagaatt taaaacatat atttgcaaac
      781 ttttgaatgg aatatgacat aaagagtggg aatttaaatt taagtttttg ttgtatatat
      841 atgaaacaag gcgaagaaat tattgattta taaagtataa attggaattg aggtgtcaat
      901 aaatataaaa ggaaatagta tttatatatg ttttggtgtt gaagtaaaag attaatgcaa
      961 agtattagag tctataaaaa tatgaatata ggaggtgtaa ttttacaata tttaaaaaag
     1021 taaatgtaat taattgtttg tataaagttg attacaagca aaagtatcag tcatagttaa
     1081 tactgttaag gatgaaggtg gtgtgttaat ttatatatgt atgggaagaa attttgatta
     1141 caatgaaaaa acttacaaaa gatatttaca aaaaaactta tagttaaatg tttatatata
     1201 aaatactatt taggggttgt ttttattatt gaaaataaga aagagtggtt ttatatcaat
     1261 cgaatgtata ataagtattg ctatattata tgtggctgtt tatttagttt ctacatcatt
     1321 gtataattgt tatagtttta tcagtagaaa tatatctgac agagaaatgt taagtacagc
     1381 aaaaaaatat atagaagatg agaagtatag aatacaaaat agtaagtatg agttaattga
     1441 agataagata gaaaaaaatt acataaatgg atatgaaatt aacagtagaa tagagcaaat
     1501 tttagattat tatcaatgct atgaaataaa tatagagata aaaaatgaat ttaaaaaact
     1561 gaggtttaat agctatgtta ctagaaaata aaggctctat attaataata acattgataa
     1621 tattttctat tataagtact gtgtgcatta tgtgtatagg tttaatttat acacataata
     1681 atatgtttga gttaggatac aaagacattc aactaaaaga aatttcttta agtggaatag
     1741 aaatatcagt aagtaatgta ctttcttgtg ttgaggaagc tattgatgta tctaataatg
     1801 aagacgaatt tagagaatat ctcttaggaa ataatatgat tgaatgtata aaaaaaataa
     1861 aagatacatc ttatagtagt ttagaagaac tttctttaaa agtagaaaat aatactattt
     1921 atgacagagg agattttttt gaatttgata tagaaagtaa tgttagagaa aaagaatttt
     1981 taaaagcaat aaaagtaaga gtgaaaatca aaaatccatg gataggaatt gacattgaag
     2041 attatatgga taaatctaat aacaataaaa ttgatgacaa aacattcaat aattataaag
     2101 catctgaaaa taatgaaatt ctaaatcaat ttaaaaatga aagtgaaaat ataaggaatc
     2161 aagaggttgg tggatttaca gaagaagatg aagaatatga agaagaactt aaacaaaaca
     2221 taattgaaaa tataaagaag aattttgatg aaaaaaacct agtacaggta tataagtata
     2281 ggggtgtata actttgaaaa aagaaggaat gtgactgtaa tagaattaat agtatctatc
     2341 agtataatac tcttgatagt tacattatct tttccaaaag ataatcttga aaatcataaa
     2401 ataaatttat ttgcacgtca actttgttca gatataaggt atgttaggcg aattaatatg
     2461 tttagtaact acaatacata tatatattat ataaatcaag gtaaacacga aggttatgta
     2521 ttgagaaaag atggtaaaaa cattaaatca ataatgctac ctaaaaatgc aaaaatagct
     2581 aggagtattg aaataataaa gtttaaatct gatgggtcgc ctcaaaaggc gtctacaata
     2641 gagatatata ataatagatt aagaaaaact ataacaataa cacctgtaag tggaagagta
     2701 cttctgaaag agggaaaata tgaaacataa atatggttac ttgcttttgg aaagtgtagt
     2761 atcattgtct tcaatggtga taattatact agttttatat tctatatttt tgtctacaat
     2821 aaacttaaag ttaaaagtag aagataagat agaactacaa caacaaagtc ttgaaatcat
     2881 aaaatctatg gaaggaatta ttggcaattc tatgggaata atgaatatat ctaattatga
     2941 agaaacattt aaaaagacaa cgtctataaa atgtagatat gtagatgaaa ataataatga
     3001 agaaggcata agtaataaag aaattatatt gaatgaaaga cgaaataagt tatttgtaaa
     3061 ttctttaaat ggcgaaagta gtcaagcagg tggttatgaa attggagatt atgtagatga
     3121 aatgtatgta tcaattacta ataatggtca atatgttaat attaaattga aattatcaaa
     3181 acgtagtcaa aaatatgaga ctgatttcaa aataaaagta tggaatttta gtgaaagtat
     3241 ataaaaatac ataaatttaa gagaaagaaa tgacatcaat aagtcatata aaatataatt
     3301 attgagaact atataactaa ataatatcaa tatgtaaagt gtatagatta agaatataga
     3361 ggataataat aaaaatatag aataagtaat ttgccaaata cttgtttata ttgtataact
     3421 tatgatatta tgatattagt aaaaatttgt aatcctgact tgacagaatt caagtaagag
     3481 atataatttt aaaggcatag taatgtaaaa tttactaaaa aataagaaat ttattttaat
     3541 atggaggaac aaaaaatggt atcagcaggt gattttagaa aaggtgttac atttgaaaaa
     3601 gatggacaac catgtttagt agttgatttt caacacgtta agccaggtaa aggagctgct
     3661 ttcgttagaa ctaaatacaa aaacttaaaa acaggagcta taagagaaga aagtttcaat
     3721 ccaagtgaaa aattccctaa ggctgttata gatacaagac aaatgcaata tctatacaat
     3781 gatggtgagt tatattattt tatggatcaa gaaaattttg aacaaatacc attaaactat
     3841 gagcaagttg aagatgcaat taagttctta aaagaaaatg aagttgctac aataagattt
     3901 taccaaggac aaccattcca agtggaagcg ccaaactttg cagagctaga agttacagat
     3961 acagagccag gtataaaagg tgatacagca agtaatgtaa ctaaagcagc tacagttgaa
     4021 actggagcag ttgttcaagt tccattattt ataaatactg gagataaagt aaagatagat
     4081 actagaacag gtgaatattt atcaagagta taaaaattta tgtatataaa agcttatatg
     4141 tcttaataat ataagatata tgagctttta gtttatatat aaaaaagaaa gcgaggggtg
     4201 ttacatgaaa catttatatg aagaagttgc atacttaaaa ggcctagcag aaggactaga
     4261 aataagtgct gaaagtaaag aagggaaaat gatacataaa atagttgatg cattagaagt
     4321 atttgcagat gctattgtaa cattggatga agaacaagaa gaacttcaag attttgttga
     4381 atctatagat gaagatttag cagatttaga agaagatatt gctgacatgg aagaagatct
     4441 ttatgaagaa gatgacgatg aggatgatga ggacttcagc tatatagaaa tggaatgccc
     4501 aaattgtgga gagttagtag aaatagacga agatttgtta tatgatgatg aagtagatgt
     4561 tgtttgtcct gattgtaagg ctgtgatatt atcatcagaa gatgattgtg atgatgattg
     4621 tacatgtggt ggttgttcaa gttgtgatga tagagagtaa aaattcataa gaattaaaac
     4681 tgcctatgat gcctatgaga tattttgctt cataggtagt tttattttta tgaggaaggg
     4741 tattatgtat tttggttttg caatacttgg acaaaaatag tacactagaa tttatagaat
     4801 aggattttgg agggatgtaa atatgaaaat aagtaaaaaa ttactagata atatacaaag
     4861 atttgaaaat gttataaatt ataaatttaa aaataaagaa tatatactag aagctcttac
     4921 tcatagttca tattctaatg aaaataaaaa atataatttt aacgaaagat tggaattttt
     4981 aggggattcg gttttaggca tagtgataag tgattattta tttaatgaag aagctaatct
     5041 gcccgaaggg gaactaacta aacttagagc aaatatagtc tgtgaagatt cattaagtga
     5101 agtagctaat gatataaatt taggtattca catgttatta ggtagaggag aagaagcaac
     5161 aggaggaaga cataggactt caatattagc agacgcattt gaagctgtaa tagctgctat
     5221 atatcttgat ggaggttttg aaagtgcaag acagtttata cttcatcata tggaaaatat
     5281 aatttatgat tcaagaaaag gaaatatatt tagagactac aaaacgcatc ttcaagaggt
     5341 gttacaaggg aatggagaaa ataatatctg gtacaggctt atagaagaaa aaggaccaga
     5401 ccataataaa agatttgtaa tggaagttgg aattaatgac gatgtacttg gtataggaga
     5461 aggcaagagt aaaaaagaag ctgaacagtt agctgctaaa atagctttaa agaaaaaatt
     5521 atgggaaaaa taaatataat atgtaaaaat ataaaaagaa atatttatat tagaaggatg
     5581 ttgtagatga aaaagagaat tatacctata tttgttcctc acaaaggatg tcctcatgat
     5641 tgcatatttt gtaatcaaaa aaaaataaca ggtgtgagta ctgatgttac tagtgaagat
     5701 gcaagaaata taattgagga atgtttagag actatagata aagatgcaga tgtagaaata
     5761 gcattttttg gtggtagctt tactgctata gatattgata tacaaaaaag tttattatca
     5821 gttgctaaag aatatgtaga aaaaggtctt gtaaaagata ttagaatgtc tacaagacca
     5881 gattgtattg atgaagatat attaagtatg ttaaaagaat ataagacaag tataattgaa
     5941 ctaggagtac aatccttaga tgaaaaagta ttattagata gtataagagg tcatcaatct
     6001 gaaattgtat acaagagttc aaagatgata aaaaatagtg gaatcaaact tggattacag
     6061 atgatgattg gtcttccagc tgacacagaa gaaaagtgta tatttacagc aaaaaaattt
     6121 gtagagctaa aaccagattg tgttagagtc tatccaactt tggtagtaaa agatacaggt
     6181 cttgaaaagt taatggaaca aaataaatat aatccattta ctcttgaaga gagtgtaaat
     6241 atagtaaaaa aagttcttgt actgttttat gttaataata tcaatgtaat aagagttggg
     6301 cttcaagcta ctgatgatat acaaattgga aaagctgttt tagcaggacc atatcatcct
     6361 gcatttagag agttggtaga agcagatatg ataaaagatt atttagaatt tgttattttt
     6421 caaaataaaa atataaagca aatgttggtc aaatctaata aaaagaatat ttctaaaatc
     6481 ataggaaata aaaaaacaaa tgttaaatat atggaagaaa aatttggtgt gcttttaaaa
     6541 acacaagaat cagatttaga tataaatcaa ttagaaattg tgttagatgg gaaaagttta
     6601 attagtgcta atatgagaga cattcataga aaattgtatg acatttatga cctttagttt
     6661 taataatgaa gggagagaaa agatttgtat ttaaaaagac tagagttaaa gggatttaaa
     6721 tcctttccag taaaaacaga tattattttt aaagaaggaa taacagccat agtaggacca
     6781 aatgggagtg gaaaaagtaa tatatctgat gctgtaagat gggtattagg agaacagagt
     6841 attaagagtc ttagagggga taaactcgaa gatgtaatat ttgcaggtac agatacaaaa
     6901 aaacctatga attattgtga agtggctctt actattgata attctgaaaa tcaattggaa
     6961 ttagatttta cagaagtaac tataagaaga agggcttata gaaatggtga aagtgaattt
     7021 tttctaaata ataaaagttg tagattaaaa gatataaaag aagtcttttt agatactggt
     7081 attggaaaag atggttattc aatcatagaa caaggaaaag tagatgaaat attaagtaat
     7141 aatcctttaa gcagaagaaa agtttttgat gaagcttgtg gcatatcaaa atatagatat
     7201 aaaaaacagg aagcagaaag gaatttaagt aatacaaagg aaaatttaga aagaatagat
     7261 gatgtatata tagaaattga aaatcaatta aagcctcttt ttaatcaaca gacaaaggca
     7321 aaaaagtact tggaaataag tgaaaaatta aagacacttg aagtaaatag ctttataaga
     7381 gaaattgaag gaatagaaaa agaattaagt gaggttaatg aacatcgtaa tgttattgaa
     7441 aaggagctca atgaaaaaga agaacaaaaa aatgttgtag agaaaaagca agaagatatt
     7501 aataaagagg tagaggtatt acaagatgta attgaaaaat ctgtagatta tataaattca
     7561 attaaaggag ttatatcaaa aaaagaatcc caaataaatt taattaagga aagaataaga
     7621 aattttacga atgaaattag tagaaaaaat ttagaaataa aggatataaa agagaagcta
     7681 aatgaaaata agcaatacat aaaagaatta gaaagcaata aattatcagg tagtgaagaa
     7741 ctatctacct tgcaggaaaa tataaaagta ttggaaggaa gtaaagataa acaaaaaatt
     7801 aagttagaat ccctaaataa tgagatagaa ttattgaaag aaagtataat tgatatatta
     7861 aataagaaac aagagtttag taataagctg tctactttaa acgcaaataa agaaaatatg
     7921 aatatcagag atgaaaatat aaattcagag ataacagagt taaacaaaaa tattgaaata
     7981 aaatcatctg aattagatac tataaataaa gaatttaata tgcaaaatga aaatttaaag
     8041 aatgtaaata atagacataa agaattatca attaatttgc aggattcaat tagtgaacac
     8101 aataaactag aagatgaaat acagaaaagt aaatataatt taaatggata taattcaaag
     8161 ttaaatgtct atatagatat ggaaaatcat tatgaaggat ttaatagagg agtaaaagaa
     8221 gtactaaaaa ataaaaattt aaaaggagtt catggagcat taggacagat tataaatgta
     8281 cctgaaaagt atgagaaatc tatagaagca gctcttggtg cttatatgca aaatataata
     8341 actgataatg agtttagtgc taagtctgca ataaactatc taaaacagaa taatttaggt
     8401 agagtaacat ttcttccatt aaatataatt aaatcaaata aaataagtct aggaaattta
     8461 aaagcaaata ctaagttcat aggaatagca agtgatttaa ttacatttga tgaaaagtat
     8521 agaaatataa ttgaaaatat acttggaaga acaatactta taaataatat agatgagggc
     8581 ataaaatttg caaaagaaac tggtcatagg tttaaaatag taactttaga tggtgaaatc
     8641 ttaaatccag gaggttcttt gactggggga agtctaaaaa ctaatgggaa tatactatcg
     8701 agaaaaagat acattaatga atatacagaa aagataagta atatcaagaa tgaaatttca
     8761 catctagaat taaaaagaga atctttagac aaagatgtga aaaatatcaa aaatgaaatt
     8821 gattcacatg aaagtaaaat aaaagattta gaaaaaagta taataataaa aagtacaagt
     8881 ataaagaatg ttgagtctga aattgaatcg ttaaaaggta gtattactaa attagaaaat
     8941 gagaagaatg atttaaattc taacctaaat tatacacttg aaaaaagtga tgatgttaga
     9001 aaagatatgg aagagttaga tgatttatac aataaaaata aagaaaaaat tgatgcattg
     9061 aatgaggaaa taaaaagata caatgattta tatgataaag aaaaatctga atttgatgaa
     9121 ttgaacttaa gtttagtgaa gaaaacagaa gtttacaata gtatagttag agatataaaa
     9181 agaatatctg gtgaaaattg tgagctagaa gaaaaaaata aacaattaga agagtcatta
     9241 aattatgaag aacatgaaat aattaaatta caagattcta tactcactga agaaaaagaa
     9301 aaagaaaatt taacaaaaca actaggggat agtaatagaa atcttgaaac tagaaaaata
     9361 gcaaaagatg atttaaaaaa tagttttgat gaaattaata aagaattaaa aactatagat
     9421 agacagcata tagaacttaa agaaagttta tttaaagttg gtggaagatt agaaagatta
     9481 aaaactagtc aagacacata tataaataag ttgtttgagc aatatgatat gaccttagta
     9541 caagcattgg aaattaaaga tgaagatttg gatatagata gaaaattctt agaaagtcta
     9601 aagagagaaa taagaagtct tggaaatata aatatagact ctataaagga atatgaggaa
     9661 ataaaagaaa gatacgattt ttatagcgaa caaaagcaag atttagaaga gtctatggaa
     9721 gagatagaaa aacttataca tactttagaa gaaaatatga aatctgaatt tgaaattaag
     9781 tttgaagaaa taagtaaaaa ttttaaatat gtatataaga gattatttgg tggaggttgt
     9841 ggagaattaa ctattttaga taaagaaaat ttattagaaa gtgatatact aataacagct
     9901 caacccccag gtaaaaaaat gaaaaattta aacctattat ctggtggaga gaaagcttta
     9961 acggcaataa gtatattatt tgctatattg attacaaagc caacaccatt ttgtatatta
    10021 gatgagattg aagcgccact tgatgacgca aatatattta gatttggaga atttttgaag
    10081 gatttatcta aagagactca atttatatca gtaactcata ggagaggaac tatggaagct
    10141 gctgattata tctacggagt tacaatgcaa gaaaaagcca tttcaaaagt tattagttta
    10201 aaattaaaag aagctcagga aataacagat attatataac ttagattgac ataggaggaa
    10261 taacagtgtt aaaaaaatta tttggttttg gaaaagacaa ggaaaaggaa atagaaaaaa
    10321 aagatgctga agaagaaata gaagttgaag acagtgttga taatttagaa aacttggaag
    10381 aaactatttt ttcaggattt gaagaagaag tgatagataa agtggaagat gttgaagaaa
    10441 aaaatgagga aagtgtcaat aatgaaggca ctgaagaaat cgaaaacttt gaaaaaatag
    10501 aagttgagat ggatagtaat ggaaaagagg tagaaaatat ttcaaataat gattctcaag
    10561 aagagatagt cgaagaatta gaaaattatg atgaaattga gtctaaagag atagaagata
    10621 aagaagataa gaaagttaac ttgtttgaaa gactaaaaca aggattgaca aaagcaaagc
    10681 aaggaataac tgatagaata gatgaagttt taaaatcata tacaaagata gatgaagaat
    10741 tacttgaaga tttagaggaa attttaataa cagcagatgt tggtgtaaat acaactatgg
    10801 atataattga aagactaaga gataaaataa agcaaaaagg tattactgaa ccaataaagg
    10861 taagagaaga acttaaatct atagttgaag atattcttac aaatgaaaac tctacactag
    10921 atatagaacc agcaccatgt ataatattaa tggtgggtgt aaatggggtc ggtaagacta
    10981 ctacaatagg taaactagca aatagataca aaaaagatgg taaaaaagtt ttattagcag
    11041 cagcagatac atttagagca gcagcaacag agcaattaga aatttgggca aatagaacca
    11101 atgtagacat aataaaacat caagagggag cagaccctgg agctgtagta tttgatgcta
    11161 taaaagctgc aaaagctaga aaaacagatg tattaatatg tgatacagca ggaagattac
    11221 acaataaggc taatcttatg aatgaacttg gaaaagtatt taaaatagta gatagagaat
    11281 ttccagaagc aaaacgtgaa gtacttcttg tggtggatgc tacaacaggt caaaatgcag
    11341 ttgttcaagc aaagacattt aaagaagttg cagatataac aggtatagta cttacaaaac
    11401 ttgatggaac tgcaaaaggt ggagtagtcc ttgcagtaaa atcagaagta gatgtacctg
    11461 taaaacttat tggagttgga gaaagtgtag aagacctaca agatttcaat gcaaaatcat
    11521 tttcagatgc attatttggt aactaatgtt cttttaaaaa taatggttga caagagtgtt
    11581 gttatgatat aaaatacaat agtgtaaagt aaataacctt tacagtatac tgctaggaag
    11641 tgtaagatat gaatatagaa aaaatggtcg aaattggatt gttatttgaa cagtataaag
    11701 aattattaac tgacaagcag aaagaaatag ttgctctata ctatgaagaa gattattctt
    11761 taggtgaaat tagtgaaaat ctgaatgtat caagacaagg tgtttatgat accttaaagc
    11821 gttcagaaaa aatactaaga gattatgaag aaaagttaca cttagtatca aagattcaag
    11881 agcaggaaaa aaatattaag attataaaag ataaaattat tgacattaaa gaagatttat
    11941 tgcacaataa agattgtgct aatttaatcc ctaagctaga aaatatagaa gatgtatgta
    12001 gggagatgat aaaatgatat ttgaaggatt gtctgataaa ctacaagggg catttagtaa
    12061 gttaaaatct aaaggaaaac ttacagaagt agatgtaaaa aatgctatga gagaagttaa
    12121 acttgctctt cttgaggcag atgttaactt taaagtagtt aaagatttca taaaaaaagt
    12181 tcaagagaga tgtgttgggc aagaagttat gcaaagctta actccagctc agcatgtaat
    12241 caaaattgta aatgaagagc ttacaagttt aatgggagat gtacaaagta aagtaatgat
    12301 ttcctcaaag cctcctacaa ttttaatgat ggttggattg caaggtgcag gtaagactac
    12361 tacagcagga aaacttggag gatattttaa aaagcaaggt aaaaaacctc tattagtagc
    12421 ttgtgatatt tatagaccgg cagctataaa gcaacttcaa gttgttggag aaaaattgga
    12481 tatacctgta ttcaatatgg gagataaaga aaatccagta aatattgcga aagcaggttt
    12541 aagtcatgcg attaaaaatg ctaatgattt agttattata gatacagctg gtagacttca
    12601 tatagatgaa gtacttatgg atgagttaaa gtctattaaa tcagaagtta agccacatga
    12661 gatacttttg gttgtagatt ctatgacagg tcaagatgct gtaaatgttg cagaaagctt
    12721 caatgaggca cttggtgttg atggagtagt attaactaaa ttggatggag atactagagg
    12781 tggtgctgca ctttcaataa gagcagttac acaaaagcct ataaaattta tggggatggg
    12841 agaaaagcta gatgatatag aacctttcca cccagataga atggcatcaa gaatattagg
    12901 tatgggagat gtcttaagtt taatagaaaa agcacaagaa aatatagact tagagaaagc
    12961 taaagaatta gagcaaaaaa ttaaaaaaca agagcttgat tttgaagatt tcttacaaca
    13021 aatggaacaa atacaaaata tgggacctct tgacaagata cttggtatga tacctggtat
    13081 gggaaatgta aaagaccaac ttggagatgt agatttgaat ggcaaagaaa tgaagagaac
    13141 aaaagccata attcagtcaa tgactacata tgagagaaga aacccaggag tattaaatgc
    13201 ttcaagaaag aaaagaatag ctaaaggcag tggaactagt gtacaagatg taaatagact
    13261 tataaaacaa cttaatgaaa tgaaaaaaat gatgaagatg tttgctggtt cacaaaagag
    13321 tatgaagaaa aggggcggct taggcggttt accttttttt aaataaaata taaaaaagta
    13381 aaaattattc ggaggtgact gtaatggcag ttaaaataag attaaaaaga atgggagcta
    13441 acaaaaaacc tttctacaga atagtagtag ctgattcaag agctccaaga gatggaaaat
    13501 ttatagaaga aataggatac tataatccaa tttctgaacc taaacaagta agaataaatg
    13561 atgaaaaagc tataaaatgg ttagctactg gtgctcagcc aacagaagta gttaaaaaat
    13621 tacttgtaaa aaacggagta atagaaaaat tcgaagcttc taaacaagca aaataatttt
    13681 ttcttggcaa ttagtttaag taagggctca ttgctaaggt tactttagga gatgaaatat
    13741 atgaaagagc tagttgtaga tatagctaag gctctagttg ataatcctga ctcagttgtt
    13801 gttgaggaat ttgaagataa tgatggtatc gtcttaaagc ttacagttgc tcaagaggat
    13861 atgggtaagg ttattgggaa gcagggaaga atagctaaag ctataagaac tgtcgtaaga
    13921 tctgttgcaa atagagaaaa tataaaagtt tctcttgaga tagtataaaa taggaggatt
    13981 aggtataatg cctagtcctt tttatataaa tgcgtagttt aagattttat gatgatatga
    14041 ccacataaga ttaaattttt taagaaggtg ataacatgaa agaaaaatta actcatttta
    14101 aggtaggtca aatagtaaat acacaaggtt taaaaggtga agtaagagtt tatccgctta
    14161 ctgatgatat agagaggttt gatgaattaa aagatttcta tctgggaaaa gatttaaata
    14221 ataaattggc tgttgaaaaa gtaagataca aaggttctat ggttataatg aagataaaag
    14281 gcatagacag catagagaaa gcagaaaaac taaaaaataa atttatgtat gtagctagag
    14341 aagatagcag agagcttgaa gaaggcgaat tttttatagc agatatgatt ggaatggaag
    14401 ttttaactgt agatggaaaa tatgtaggga tactagaaga tgttcttcaa tactcagcaa
    14461 atgatgtcta tgtagtaaaa ggtgaggaag ataaagaatt tatgatacca gctataaaaa
    14521 aatttgtacc tacaatagat attgatgaaa gaaaaatgat tatagaccct attaagggaa
    14581 tgatagatta ggtggtaata tgagatttca tataatgaca ctttttccag aaatatttaa
    14641 ttcgtacatg gatgaaagta taatgaagag agcagttgaa aaaggtataa ttgaggttca
    14701 tatatataat ataagagatt tttctaataa taaacataaa aaagtagatg attatccatt
    14761 tggaggagga gcggggatgg taatgactcc tcaacctata tatgacacat ataagcatat
    14821 aataacaact cataatataa ataacccaag tgtaatttat cttactccaa aaggtaaggt
    14881 atacaatcaa tctatggcaa agcagatgtc tttaaaagaa gatataatac tactatgtgg
    14941 tcattatgaa ggaatagatg agagaatcat tgatttaata gttactgatg aaatttcaat
    15001 tggagattat gtattaactg ggggagagct cccagcactt ataatgatag attctatatc
    15061 gagattaata ccaggtgtat taaaccaaga agaatcattt gaagaagaat catttaaaga
    15121 taacttattg gaatatcctc attacactag acctagagat tttgaaggat taaaagtacc
    15181 agaagtgctt ttatcaggga atcataagaa gatagatgag tggagaagag aagaatctat
    15241 aaggataacc aaagaaagac gatttgattt atataaaaaa tctaatgaaa aataaaaatc
    15301 taatgaaaaa ttaatatata tgtatacaat aaagtaccta taaatggaat taaaaacata
    15361 aaagtgcttg taataactat tgaagtatag tataatataa aatgttgaaa atacgggcgt
    15421 tcctctgttt acactggtcg ggtagttaag aacgtctatg atatctagga ggaagaaaga
    15481 tgaatgaaat gctaagagca attgagcaag aacaattaaa aaatgaagtt cctaattttg
    15541 gacctgggga cacagttaaa gtacacgtta gaattataga aggaaaaaga gaaagaatac
    15601 aagtattcga aggtgtagta ttaaagagac aaggtggagg agcaagagaa actttcacag
    15661 ttagaaaaat gtctttcaat gttggtgtag aaagaacttt cccagttcac tctccaaaaa
    15721 tagaaaaaat agaagttact agaaaaggta aagtaagaag agctaaacta aactacttaa
    15781 gaggtagagt aggtaaagct gctaagataa aagaagctag aaataaataa ttaaaaactt
    15841 tacttataaa tggggggctg atttatcagt cctctttcta ttttaatgaa aattacagca
    15901 aataattata ctagaaataa atagaaatat ttgttgaaaa tatacattat ttatctatga
    15961 atttatagct tttaaaatga aaaataaaat tggtaaatat tgcattatat tttgtaacaa
    16021 tattaaaaat aagagaacag tattatgtaa agtatgacgt taaaaagatg acaatattat
    16081 agcttgttaa gatagatttt ttaagtggaa ttaggatata ataatataat attaataagg
    16141 agtgatacat gtgtcaaatg aatacgaaga gtatcttatg aatgacaatc tacatataaa
    16201 ctggtatcca ggtcatatga aaaaaactaa ggagttagta aaaaataact taaaattgat
    16261 tgatgtagta attgaacttt tggatgctag aatcccgttt agtagtaaaa atccagacat
    16321 agacaggctt gtaggagata agccaagagt tgtcgtactt aataagagtg atatggcaga
    16381 tagggataaa ttgaatcaat ggattgaata ctataagaaa attaatatca aagctatacc
    16441 agtagataca ataaaaggcg ttggtataaa taagataatc gaagaatgta aaaatgtaac
    16501 tagagaaaaa atgagttctt taaaagacaa agggagaaaa gaaagagcta tacgaataat
    16561 gatagttgga gttccaaacg ttggtaaatc gtctcttata aacaaattaa caggtagaaa
    16621 aagcactcaa actggagata agccaggagt aacgaaaggg aaacaatggg ttagattaaa
    16681 gggaaatctt gaacttttag atacacctgg aatactttgg cctaagtttg aagaccaaga
    16741 agttgcatta aatttagcat ttagtagagc tataaaggat gagattttag atactgaaac
    16801 tttggcttta agacttatag agaaacttat gaaaatagaa cctgagaagt tgaaagccag
    16861 atataagtta gattgtctag gagaaactcc aattgaaact atggatatga ttggccataa
    16921 aagagggttt ataacaggta aaaaagaact agattataca cgcatagcca caactgtatt
    16981 aaatgaattt agagatggaa aaataggaaa tataacattg gaagttccag aaaacgttaa
    17041 aagatagatt ttaaaacaaa atcaaaaatt taatgttaaa aatttaaaaa agccttttag
    17101 caaaggcttt tttatttttt tgaaatatag acaaatatct ccatataaaa gtataaatta
    17161 ggttagaaaa cctttaaaaa tattaattta agacaaatat acctaatatt ttgaaaatta
    17221 aaatgagtgt gattatttaa aattttagca tattgacttg cttgtttcgt ataatatata
    17281 ataagcacaa ataaatacaa tatattaaaa atttaattta tttaatttta ttaatttaat
    17341 aataagttct tattgtttaa ttcaacttac ttatgcaagc gtaaacaatc gagacatttt
    17401 attaatttaa taaggttaaa aagggggatt ttataatggg taagaccaag gatgttatag
    17461 tatttggatt tgcgttattt gcaatgttct ttggagcagg taacttaata tttccacctt
    17521 acttgggaat aattacaggt ccagaatggt taattgcctt cttaggattt acgtttgctg
    17581 atgctggatt ggcacttcta gcagtaatgg ctacagctaa attcgatgga aacgtagttg
    17641 aaatgtttaa aagatgtggt atgaagttag gaattcttat aggttgtgca gatattttat
    17701 gtataggacc attcttagct ataccaagaa caggtgcaac aacttatgaa atgggtataa
    17761 tgcctttgtt tggaacttca attccagtgt tattattctg tatacttttt tttgcgattt
    17821 catatgtact aacaataaga ccttctaagg tcgtagatat agttggacag tttttaactc
    17881 cagctcttct aatagcttta gcatttataa ttataaaagg tattatatca cctcttggag
    17941 atatagtaga taaacctatg atacctaatg tatttgcaga aggtataggt caaggatatc
    18001 agacaatgga tgcttttgca gcaatagcac ttgcatcagt gctaatagta tctttaaatg
    18061 ataaaggata ttctactatt agtgacaagt tgaaaatgat tggtaaggcg ggtgttcttg
    18121 cttgtggagg attagctcta gtttatggag gattatgttt ccttggggca actgtatcaa
    18181 caatgtatgg aacagatgca gtacaatcac aagtaatagt aaatataact gaaggattgc
    18241 ttggaaatgt tgggaaagcg attttagcag tagtagtatc attagcatgt ttaactacat
    18301 ctataggtct tacatctgcc acaggtcaat atttctctag acttacaaaa ggtaaattga
    18361 gttatgaaaa aatagtatta gctgtatctg tattcagtgc agtagtagca tcttttggtg
    18421 ttggaactat aataaagata gcatctccta tactaagtat agtttatcca ccatcaattg
    18481 ttttaataat tttagctttc ttcaatgaaa aaattaagaa tgataatgta tacaaaggag
    18541 cagtatatat gtcattgctt gtaagtatac taacagttat atctagttat ggagtagctg
    18601 ttccagtagt aaattcttta cctcttaatt cattaggatt taactgggtg gtgccagtta
    18661 taatagctgg aataattggt aattttatac catcaaagag tcaatctaat acacttggaa
    18721 caaactaagt tttgtttaat taaaatataa tcaattgtaa ataaatgagt taactaaaag
    18781 taaaaggagt tatttgtaca aataactcct tttattttta gattctatat ttaa
//
LOCUS       DAENCV010000069        18750 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAENCV010000069 DAENCV010000000
VERSION     DAENCV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.070"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            101..1003
                     /locus_tag="KQD82_002427"
     CDS             101..1003
                     /locus_tag="KQD82_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG4380894.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            1615..2628
                     /locus_tag="KQD82_002428"
     CDS             1615..2628
                     /locus_tag="KQD82_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG4380895.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2980..3549
                     /locus_tag="KQD82_002429"
     CDS             2980..3549
                     /locus_tag="KQD82_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG4380896.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3885..5222)
                     /locus_tag="KQD82_002430"
     CDS             complement(3885..5222)
                     /locus_tag="KQD82_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG4380897.1"
                     /translation="MGIYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(5747..6457)
                     /locus_tag="KQD82_002431"
     CDS             complement(5747..6457)
                     /locus_tag="KQD82_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380898.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            complement(6841..8952)
                     /locus_tag="KQD82_002432"
     CDS             complement(6841..8952)
                     /locus_tag="KQD82_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4380899.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(8942..9694)
                     /locus_tag="KQD82_002433"
     CDS             complement(8942..9694)
                     /locus_tag="KQD82_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380900.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(10767..10943)
                     /locus_tag="KQD82_002434"
     CDS             complement(10767..10943)
                     /locus_tag="KQD82_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380901.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(11047..11655)
                     /locus_tag="KQD82_002435"
     CDS             complement(11047..11655)
                     /locus_tag="KQD82_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4380902.1"
                     /translation="MKKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(11754..12578)
                     /locus_tag="KQD82_002436"
     CDS             complement(11754..12578)
                     /locus_tag="KQD82_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4380903.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(12756..13649)
                     /locus_tag="KQD82_002437"
     CDS             complement(12756..13649)
                     /locus_tag="KQD82_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4380904.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(13906..14343)
                     /locus_tag="KQD82_002438"
     CDS             complement(13906..14343)
                     /locus_tag="KQD82_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG4380905.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVYDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(14600..14749)
                     /locus_tag="KQD82_002439"
     CDS             complement(14600..14749)
                     /locus_tag="KQD82_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG4380906.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(14762..14992)
                     /locus_tag="KQD82_002440"
     CDS             complement(14762..14992)
                     /locus_tag="KQD82_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG4380907.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(15851..16072)
                     /locus_tag="KQD82_002441"
     CDS             complement(15851..16072)
                     /locus_tag="KQD82_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG4380908.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            16652..16909
                     /locus_tag="KQD82_002442"
     CDS             16652..16909
                     /locus_tag="KQD82_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4380909.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            17378..18615
                     /locus_tag="KQD82_002443"
                     /pseudo
     CDS             17378..18615
                     /locus_tag="KQD82_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
BASE COUNT         6932 a         2149 c         2638 g         7031 t
ORIGIN      
        1 gggatagcct gtggagaatt agtaagacat atgttagtat gcaaaattct atgaagcagg
       61 aaccctgtga ctttagtcat gggaggttca gattggaaat gtgttgccac aagattatta
      121 tggattaata gtgattttag gtgttattct tggagttatg ggggcatttt acaactggtt
      181 tactctatat gtgcagtctt tatacaagaa gattcctaaa attggtacct ttggaaaact
      241 gttgattcca tttataatgg ctggtattct tgggattgtt atgcctgatg ttctggggag
      301 tggacacaat ttagttgaag aacttacaag tcatgaatat atttttggaa tggttgtttt
      361 gatttttgtt atacgtttta tattttcggc tgttagtttt gggtcaggtg caccaggggg
      421 aatatttttt ccattgcttg ttttaggagc atttataggt ggaatatttg gtatggtagg
      481 tgttaaagtt tttggaatga atcccgatta tgtaaataac tttgtgcttt tggctatggc
      541 aggatatttt acagctattg tgcgtgcccc tttgactggt atcatattaa tttttgaaat
      601 gacaggttct gttagtcaaa tgctttcact atcagtaatt tcaattgtgg catatattgt
      661 ggctacactt atgaagtcta aacctattta tgaaagtctt cttgagaggt tacttgaaaa
      721 aaatggagaa cctgtacata aagagagggg cgaaaagata ctagaccagt tctctgttat
      781 gtttggctct cctgttgcga acaaggcaat tcaggatgta cagtggcctg aaaattgtct
      841 tttggtagca attcaaagag gtggagaaga aattattcct aggggtaaga caattcttag
      901 accaagtgat gtaattgtga ctatgacaga tgagagagat agtggtgctg tgtatgataa
      961 aatggaaaat ttgtgtaaag aacatacggc acatattttt tagggataat gatgaatgtt
     1021 taataatgat agaatattta atgaagattt gattaaaaaa ttggtaattt gaaagagagt
     1081 taaggaatta atataagaaa atgaaaatgt gatatcttag atgtaaaaga gtttgaagaa
     1141 aataattgta tatatttgtt aatgagatta tatggactaa tggtatattt atactgtgct
     1201 aaaagttgca ctattgtatg aggaatggga ataaagggtt tgaaaaaata ggtagaagat
     1261 ttttcagata ttcttattgg gatattttaa ttaatatatt ttaattgcga tattttggga
     1321 agaatagaat ttatttataa atgaaaataa ttaaaagggg ttaaggttgt attagcccct
     1381 tttgcttata gagtttggaa atggtatctt gatagtagtt gtttcatgct tattatattt
     1441 tattacatat tttaaaattg accttattac tcctaaatca ttgttatatc aacttttaaa
     1501 acactttcaa gacaatcgcc tattgaaaac aaagttaatt aaaaacctac atatcagttt
     1561 agtattcgtg tagatgatta tataaatata aaagtataag aaaggtgagt atctgtggtt
     1621 agttcatttt taaatgatat ctttagaaca attttagtaa gttgtatttt tatagttatt
     1681 ttattagtct ttagaataac tttatttaag attttcagta aaaaatttaa ttactatata
     1741 tggcttattg tgattattaa gctatcatta ccatttatga attatacttt tatatttaat
     1801 gagtcaaaat ataatggtaa tattaataag ataagtttag gcaattttaa tagtatttct
     1861 attgtatata gtactgtttt agtctctgtt tggattgcta ttacgatatt ttatttagtt
     1921 tatactttat tgaaatatat taagcttaaa aatatgataa atgatttgtc ttatgatgtt
     1981 gaggatgata agataaaaaa tctttatgaa aatttattaa aagagcttaa gataaataaa
     2041 aaaatacggc ttaaatattc atatgaagtt gaaagtccag tattttttaa ttcgtgtgtc
     2101 attttaccac catgtgatta tacattgaaa gagttaaact ggatatttag gcatgagttg
     2161 atgcatttta agagtaaaga cctttatatt aagtatttgg tgatattttt aaaatgtgtt
     2221 tattggttta atccttttgt gtatataatg gagaagttta tagatttgga ttgtgaattt
     2281 tattgtgatg aaagagtttt gaagaattgt acgatagaag agaagcagga ttatgcattg
     2341 acaataatta gtgcaatgag aaaaggttca aatagttcaa ataaatttgt ggctggatta
     2401 cataaggagt cagatattaa aaaaagagtg tttagtatgt ttaatgaaaa gtacagaaat
     2461 ggtattttga tagcagttat tttatgttta ttatcttcta ttacatattt taaaattgat
     2521 ttaattacta ctaaatcaat attttatcca ctttcaaaac aaacacctat tgaaaacaga
     2581 actaattcaa aacctacata tcagattaat gtttatatag ataattaaaa aatacaaagt
     2641 ttaacaagag attaatataa ataattttaa taagtatctt tcacatatgt taaatttact
     2701 ttaattatat taaagccaat gaattattct ttagaagctc caatgccagt aaatacaggt
     2761 aaagaaaaaa ttactacaat tgtagattaa aaatgttcta atatttaaaa aaatgcaaaa
     2821 gtgtaagaag ggtaaaaatc tattataata cattaaaaac taaatttaga aaaatttaca
     2881 taattagaaa aatgatttga taaaaaataa agttaaatat taactaagta aatttgaaaa
     2941 agaaaaaata taaagtacaa atttaaaagg aggtaactaa tgaaaaagaa gttggaagat
     3001 atgactttag aggagttatg gaacttgttt cctatatttt tgactactca caaagaatgt
     3061 tggaaaaatt ggtttaaaga agaacaatat agactaactg gaatattgcc aatgcaaaaa
     3121 attgttagaa taagtcatat tggaagtact tctgtgagta cgatttatgc caagcccatt
     3181 gttgacatac tgattgagat aattcctgat tataatatga aagatattaa agatttgatt
     3241 gagtcgaatg gatatatttg tatgtctgag agcagtagta ggctttcact taataaaggg
     3301 tatacagtag atggatttga agaacgagtt tttcatttgc atataaggta tgtaggtgat
     3361 aatgatgagt tgttttttag agattactta attgaccacc cagatgttgc aaaggagtat
     3421 gaaaaactta agttggacct ttggaagaag tatgagcata atcgtgatgc ttatacagat
     3481 agtaaactag attttgttag ggagtttact gaaaaggcaa aggtgttgta ttctaataga
     3541 tataattaga aataggtaca attagatatt tatcttatat attgtattat attttttaag
     3601 aaagtaatta atagaaaaag ggcttgtttc aaaatggaga gataaatccc tttttcattg
     3661 ttgaaaggat aaaaatatat ttgatttcat gtaaaaaaga gtgcctcatc aactaaaaag
     3721 tttattttga gacacacaca tttttactaa ggtaaataaa atatcacatt tgattaaatt
     3781 ttaagttaat gaatatatgt gtaaatattt aattataagt tattataatt ttacatagta
     3841 tcaaacaatc taatttgtat tatataagat aatctaattt tagactaaaa taatctaata
     3901 taaagaaata aagaaaattc actctcagta taagagatat ccaattttcc attgtactta
     3961 ttaacaattt ctctaatatt ctcaatacca tatccgtgag aattactatc ttttttatct
     4021 gaaataaata gttcttgttt tctattgata ggatttacct tgttgtttgt caataaatag
     4081 ctaaaatact ccttctcaca acgtgccttt aataccactt ttctatttga caaatcagga
     4141 attttcaaag atgcttctat agcgttatct aaagaatttg aaaaaatagt acataaatct
     4201 ataggttcta tacccaagtg attgtctata tctatattaa agtgcacatc aacaccattt
     4261 tcttctaaac ttgtatgacg aagatttaaa attgagttaa gagcttggtt tttgcaaaat
     4321 tgttttccac taagagtacc tatacctatt tttaactgat taaaatactt tttagctcca
     4381 gcaatatctt caatttctaa atacgattca atcatttgaa agtgattatt catatcatgt
     4441 cgcattttgc gtatttgaat ttgttgattt tctagtaagt tatagtattt ttcttttagg
     4501 tgatttgctt gtttttctat cttcatcttt acattttcaa tcaatggctg taaaagaagt
     4561 atcacaccga tatttgccag aattgctgac aagatgatta gataagtgcc ataaggtaag
     4621 ttgcctaaga taatattctt attactaccc atagggatta aattagtagg tggcagaata
     4681 atagcaatta atattgataa aaatgaagat atacatacca tatcaataaa aatccatatt
     4741 ttatttgata agtattgttt tatattgacc aatctattct ttaggaaaat atgtattaaa
     4801 aaccaaaata ataatttaat tagagcagaa attatctgga ctattgaaag tattaaaata
     4861 tactcttttg agtgtataag ttcctcatat atagggttgt ttataaaaag aaaattaagc
     4921 ccaattataa taggataaag tatcatgaca actgatatta taggaattaa tttgcttttg
     4981 ctacatatta taagtagtat aaaatatcca aatagtgtaa acaaaatatt tacggggtcg
     5041 gaaagccata ttatcatttg actaataata gagtagccca aaatcaatat gattttgaaa
     5101 atccaatgat tcctaaagtc tataaaatag gaagtcaact tataaagaaa aaaggctatg
     5161 aaaatattga ttaatattag aataaaattt atgcttgtag agattaaaga ataaatgccc
     5221 atttaatata cctttctatt aattaagatt ctggaaacta agttttaaaa aattaaatct
     5281 taaaaattaa aattctggaa attagatttc aaagaattga ttttgaaaaa taaaattcta
     5341 gaaattaagc ttcaaaaaat tgagttttaa aagattgaaa ttttaaaaat taagtttcaa
     5401 aaaattaaat ttgaaaaaaa actctaaaaa ctaagcttca aaaaattgaa ttttgaaaga
     5461 ttgaaattct agaaactaag ctttaaaaaa ttaagtttga aaaaaactct aaaaactaag
     5521 cttcaaaaaa ttaaattttg aaagattgaa attctagaaa ctgagcttta aaaaattaag
     5581 tttgaaaaaa actctaaaaa ctaagcttca aaaaattaaa ttttgaaaga ttgaaattct
     5641 agaaattaat cttcaaaaaa ttaaatttga aaaaacaaaa ctctaaaaac taagctttaa
     5701 aaaattaagt ttcaaaaata aaattctaga gattgaactt caaaaattaa gattctaaca
     5761 tataccttgt aaattttcct acaacctctt gatatttttt acggcttata ggaatttttt
     5821 cattctgaac tttggcataa gtttgagtta tactatctac atggtttaga ttgacaatat
     5881 acctttgatg cgtacgtata aaattagagg gaagaacagt ttccatatca tttaattttc
     5941 cgtagtattc taatgtttta tcaaaggtaa ctacagttaa ttttcttagc tcacttttaa
     6001 agtagaggat atcttttgta ttaattttac aaagattagt tccaatctga actgtaataa
     6061 agttttcttt ccattgttct aattttttta gtgcttcttt taatacttta catatttttt
     6121 cagttttata aggttttaag atgtagttca aagctcctac atcatatcca tgaaatacat
     6181 agtcagaaaa acctgtgaca aagatgatta tagtatcctt tgaatgtttt cgtatagttt
     6241 ttgctatgtc aacgccatta ctttttccaa gttcaatatc taaaaaaata atatctatgt
     6301 aataattttc gtgttctaga ttgtatacta gactttctcc atttgaatat tcttttattt
     6361 ggtaggggta gccccacaaa tccatttctc ttgtaatgat tttattcagc atgtttcttt
     6421 gttgtttttc atcatcacaa atacaaatcg aagtcaattt tttatctcct ttcaaatttt
     6481 atatttatat agttaagtat ataatatatt ttgctaccta gatatataaa atgaaataac
     6541 aattatatcc agatagcaaa ataaaatttt ttatattagt atacttacac ttaaaactat
     6601 tctattaaac ttattctgaa gtgctaatga ttttcttatt aaaaaatcta acaatctttc
     6661 ttattataaa atacaaatgc ttttattatt ataaaaagta atgattttat tactttaaaa
     6721 atctaataac ctttcttatt ataaaacacc aatgatttta ttactttaaa aatctaataa
     6781 ccttttttat tataaaacac caatgatttt attactttaa aaatctaata acctttttta
     6841 ttataaaata ccaataattt ttttgaattt cttataagtt aatagatata taaggtacat
     6901 ataaacccca caaatcccta taaaacctaa gtttatcaaa agagcattga cctcactatt
     6961 gacgattcta ccatcattat ttaacataat cataaaatta aacagataaa ttaaaaaact
     7021 tcctgcaata agagggtatg aaaatacttt ctttaattga tttctaatac agaactttat
     7081 ataatcattg tttgctccta atcgatttaa atccataaag acctgcttgt gatttatagc
     7141 tatagtaaca ccccttgtat atgcaatgat tcctactgct gtaaaacata tgattgccac
     7201 atatatgaat aacattaaga agactgccat gttttttatc atatcttggc tttgtaaaat
     7261 tttaaagtta ggatagtatt tccaattaag atacaactga ttgttgtttg ggtctagttt
     7321 taaatgacct tcaccacttg gatattcttc atccataaaa tattttcttc cagttgattc
     7381 ataacgtttt tttacgtatt catcataacc actgaacatg cccatttcct tagatgttct
     7441 ttttataatt tctttcttta agtcattggc aaaagcatag gtatctttat ctacattaaa
     7501 tagtatagaa ttattgtaat tttttatgga taaatcttta gtaaatgatt tataatcctc
     7561 atcattaagg acataggcgt tcatacttct attttgaaaa aaagaaccat taaatattat
     7621 ttttccagca tatctaaagg ggatttcttt ttctgtgact gggcttgata taaatttggt
     7681 atatttataa tcttgatttt catttccttt atttacaaca gcaagatatt caccattttt
     7741 tatagttatt ggtttgccaa ttagcttgga aaaatcttta gaattaaaga acatatcata
     7801 ggagcttttt ttcatatata ccttttcgat agttccatct ttatgatata ttgattctac
     7861 accatcaaca attaattcta tagaaggaat ttccatatag ttttgaattt ttatattgtg
     7921 tttatctgct aattgttcaa tatctttttt tgatacctgt ttatctcttt gattgtaggt
     7981 aaaagtataa tctatagggt tatttttaag agtatcatag atacctgtaa ccatattagg
     8041 aacatagaat acagcaaata atgctcctgc aatcaaaaat gaaataacac acatgttttt
     8101 tactgtttgt cttcccataa atctcatcat gctagttgat atgatgtttt tatagtattt
     8161 ctctggatgt tttcctcttt tagcatgtac aactatgtat gtcataatca tatatattcc
     8221 aataagagag agtagataag taaagctcca tacagatggt agtctataat ttaatttgtt
     8281 tatagttagt gaaggtacga gatatcctag aaaaatacct agtataataa aaattatacc
     8341 taatatacca taccagtcct ttacatcctg tattggttca gatattctct ggtcatttaa
     8401 tatatcaatt atatttgtac gattaataaa ttttacagaa agtataaata tgcataatgt
     8461 cacaaataca ctaaatataa atccatatat gaatccttga gttccaaaat gatatgtcat
     8521 ttctctggta tcaataatca ctaattgaaa tattttccat ataccaaaag atacaggaat
     8581 acttaatata agacctaaaa aacagcatac tactgaaatt aacaatatct ctgaaaatag
     8641 aagttttttt aatttcttct tatctgttcc tagagccatg aaaataccaa tttccttgct
     8701 tttaaatttt aaaaagaggg aagatgcgta tgtagtaaag acagtgcatc ctaatatagc
     8761 aactgagaat atcattgtag cctgttttct gctatctcca ccagttggaa gtatgttttg
     8821 tattgttggg ctaaaataaa ttagagcata tgaagttact aataccacag aaagtattat
     8881 acaaaagaat aaagttgtat agttcttcca attataggct cgaagtttac tgtataattt
     8941 attaattgtc atcttgagcg cctcccattt cttttaaaac ttctagtaat tcatctagga
     9001 atttatgtct tgaatttgat tttacaactt ctttataaat tattccatct ttaagcacaa
     9061 taactcggtc acagaagctt gctgcaaagc tatcgtgtgt aaccatgagt atagtagcat
     9121 ctaattcttg ctttgctttt aaaaatgatt caatgactgt acgacaagac ttactatcaa
     9181 ggttaccagt tggttcatct gctaatatta aatctgggtt gttcatgagt gctcttgcta
     9241 ctgctgtacg ctgtttttct ccaccagata tttcggcagg gtattttttt ctgatatgtt
     9301 caataccaaa tagctcacat agattaattg cacggttttc catttgtaag acttgactac
     9361 ctttgataat ttgtggaaca catatatttt caaagacact aagaccatca aatagcatga
     9421 agtcttggaa tacaaatcct agttgttgat ttctaacttc tgaaagttcc ttttctttta
     9481 aatttcttat attattattt ccaagaatta tttccccatt atcaattggg ataaaacatg
     9541 aaatacaatt taaaagagta gtttttccac ttcctgatga acccatgatt gcgacaaatt
     9601 ctcctttgtt gatttcaaaa gaaatatttt ttaatacagg atatgtttta ctgcctgttt
     9661 tatatgattt acataattgg tttactttaa gcatatatat catcctcact ttcatttatc
     9721 ttgtaagtca atgatagcat ttgaaagggg aggagggata tgagctatat tgtaatagtt
     9781 ggatgttttt ttgtaatgtt tgaaaatata ttgtttggat agtgtgagag gttattatta
     9841 tagttaaatt gattatatta ttggaggatt tagagggtta tttttaggta ttaattgttg
     9901 tgctaaagta atatgatgtg gaacattgaa ggtgtagtta gaaaagaagt atatagtaaa
     9961 agtaaaaagg ttatagaata acgaatgttg tattgaaaaa aatgatgtaa ataatgtata
    10021 gtagggttaa aaagaaaaaa agaattatag agcagtaaga ttatttaatg gaatgtattg
    10081 taaataattt gaaaggattt aggggctatt aacacaatgt aattgagaaa atatattgga
    10141 gttttggaaa taaataggtt gttaaaaaca attgaaattt ataataaaaa ggttaaaaag
    10201 tattattagt ataggtatga tagataattt tagaaagtgg aagtatttta gactatgctg
    10261 aaaagtgatt gatatgattt tgagatatta aaatattact atattatagt aaagaagtta
    10321 aaataataaa atgttgtaat aataaaggtt tgtagtataa taaagaactg taataaataa
    10381 gactttgcaa tgtaatagaa atgctgtaat aataaaggtc tgtagtataa taaagaactg
    10441 taataaataa gactttacaa tataatagaa atgttgcaat aacaaaatac cataataaat
    10501 aagagtttgc aatataatag aaatactgta ataacaaaac actgcaataa ataagagatt
    10561 gtaatataac aaaatcctat aataaataag attttacaat ataataaaaa tcctataata
    10621 aataggactc tgtaatataa taaaatgctg taacaacaaa acactataat aaataagacc
    10681 ctgtaatata ataaaaatac tataatatag taaaaatcat gcaataataa aaatctataa
    10741 taataaacct cagcataaca aagacattaa tttcttacta aaaaggccac cacaattaac
    10801 agtgaaaaag caattgaaag tattgaaaaa gtaaagtgag aattagagtc attattacga
    10861 ttattaagtt ttatccataa accacatata attgtacaca acaataaaat tgctgatatt
    10921 actcctataa atgtaaaaaa cattaaaatt cctccccctt atttaatgca agtattttta
    10981 gtcaatgaga atattttcaa acgttaccat aaaataaaaa aatgtacata aaaaattaat
    11041 atagtattat tttgtaatat ctaccaataa taaatctaat tgaaaatcta ttaaaccatt
    11101 tctctgttca acattaagaa tatcaatacc actatacttg aaaaaagcat tagaacgaag
    11161 aaaaagcaac tgaataaatg aagttatttg atacgcaata gttctgtatt cagcttcagt
    11221 ttttttgtta ttctgatttt catagtgagc cttaattaaa ggtagtatct caaatggata
    11281 aacaatttct tcctgtatca atatataggg tacagaaatc tttataaatt gactatgttc
    11341 acctataaaa tcaaaaaatg caattagtat ttcccttaat tgctgtttag gtgctaaatt
    11401 tttagtatca tgtgtagcct tatattcatc catatgacgc tttaaaaatt ttttaataga
    11461 aatattaatt aactcctctt tagatttaaa ataataatta atcatagcta aattgacatt
    11521 tgctttagct acaatttctc tagccgtaat gcttgaaaca tctctatgtg aagatagtaa
    11581 ctggattgtg gcattaatta attgttcctt tgttgaatct gctttcataa tttttcccct
    11641 taaaagtttc ttcataaaaa aattaaacat gtttaataca cgtttagata ctatcatgaa
    11701 aattttggga tgtcaatggg tgattagtac ttaagtattt aagtactaat cgattagact
    11761 tttataaggg ggatttgaat ttctgtaaga taattttcag gatttatttc attatgttca
    11821 cctttatgac cgatttgtct atttatacct gatatttgat aatcactatt tttatctaac
    11881 caataacaga acgattcata tgcaccagct atattttcat atggaccata aaccatagta
    11941 catgccatca tatcaatttc ttctgtctca cgatatataa atccagattt gttctttcca
    12001 atcttcttta caaccattcc aacttcaata tctacaccat tatctttgac ttcttcatca
    12061 tggtaaaaag ctatgttatt gttggattga cgtgatactt ctatatgttc ttctttgata
    12121 aatttagata gcttttccca gagtatacct tcagattggt agtctggaat tatctctcta
    12181 agtgatatga ttttataact tggaatcttt ttaaagacaa cattgtaatg aatttcatct
    12241 ttatcacagt ttatagcttc tataaattta tcaattttat ttattctttg ttgttcctgc
    12301 tttatttcct tttgtatttc attctttttt ctattcaatt ccttaattac aaattcatca
    12361 ttccaattat gtacgatatt tgctatttct gcaacgctaa attttgaatc tctcaataat
    12421 acaattcttt gtaatattga aatctgttca actgagtata atctatgacc tgtatatttg
    12481 tctgtttttg caggttttaa aataccaagt tcatcataat aacgcaacat acgaatagaa
    12541 atttgtgtta gttttgaaac ctctcctatt ttaaacatga aaatatgacc tccttttcac
    12601 ttataatcag tatatatttt gtttttatat acttcaagcg tttgtggaaa tacaattatt
    12661 ttccagatgt tacaataaat tatagagagt aaattatatt taagtagtaa tcgtatcaaa
    12721 tcatagatta tcttaattta taattcatat taaaattacc agtctaactc aaattcaaaa
    12781 tcatccgact ttatctgttc aatttcagaa tttaccttct gtttaaattt attagaagaa
    12841 atcatatata caagaaatat taaaccaaat attatttgta catattgagc tattacacat
    12901 aatttaatag ggtagtgtag aagtaattga atgttgaaaa aatagtacat catatgaact
    12961 aacccagctg ataaaatgct tctacttgta ttgatatata caaaattaat tataaagttt
    13021 aaaagtacaa tgtttggtat aaataaaaaa gcatcaaata gggagtattg tgccaaatta
    13081 tatggtattt gattaggtgt aaaatacgac cctagatacc agattccaca aacaagccct
    13141 aaaataattg aagagcctat aaatccaaat ctaactaata atttgtcaat ggcatatcct
    13201 ctccatccag cttcttgact aagtatactc aatataaaac ttgctaatag catataagct
    13261 attttagaag gtctaaacac tgctatgtgt atccaatcta tatcagaagt ttttacatta
    13321 aagtaatttc cgattattaa cgaagtaata aaagttaaag caaaaaataa tattgttaat
    13381 agtatccatt ttattctaat ttctttaaag cttaggtatc tataaataaa atcttgtttt
    13441 gcactttgtg gatagattgt agaaataaaa aatagaccta caatagctgg tgatgtatat
    13501 gcaagataaa aaatgatttc tccaagtatt gtatttgtaa ttccgagcaa cgcaggtgta
    13561 agactgaata tccagttcaa tactagcgta aatgtaaaga aatagattaa gtgtttgttt
    13621 gtattaaagg gggtgttata tagttgcatt ttttcactat ccttccgttt tataatgaag
    13681 ttttaatttt tattaattgt ttatttattt ttatatgtat taagttatcg cttgatgtta
    13741 gttattgcct ataataatat cctatttttt atattttgat taattatttt tacactaaat
    13801 ataattaatt agtatttaat attgaatata ttatattatt attaattatt aaataggtca
    13861 ataaatttgc aattagtgat attaataaat tactttagat ttttattaaa tgtttagctt
    13921 ttctttaata ttttttatat aacgacgatt tataataact ttttcgttgt tttttaaagt
    13981 agcttgcatg cgattatgtg atattacttt tatacttttt atgtagtcca tatttaaaat
    14041 catggattta gatattcgtg agaaggatgt attagctaat aattttgata atttatatat
    14101 ttttaaatca atttcaaata catcatcttc ataataagca aatgttttat ttccaactgt
    14161 ctcaaagtat aaaatatcat agacattgag tttataaatc tctcctgatt ttttaccttt
    14221 tatatcaagt aacggtgtac tcaataatga aattattttt tcaatgttat tatctagagc
    14281 agaacatttt atggttattt caatatcatc acaattaata ccttgttcga catgtatttt
    14341 cattaatcat cacctcattt tattactgtc attagaatag cattatatct tattgagtcc
    14401 aactagatgc gttctaaatt tcaaattaag catagtgaaa ttcaaaaata aaacagtgaa
    14461 attcaaattt gaggtggtaa aattcaaaaa aatactatgg atatttggat aaattatcca
    14521 aatatcctag cattttacat agaatatgcc taattatttt acaaatagac ctattttatg
    14581 acttacatat attgtcaaat tacttcttaa atatcaaaat atttttggat aatgtatcaa
    14641 tactgtttga taaagtattt aatttaccct caattctaac caataagtac atggatagag
    14701 ctataggaaa accaacattg gttattaaca gttgtaaact atcatccata aaattagccc
    14761 cctattctat aactaagtca taaggagtgg tatttgtttc cacaaccttg gcttcaacca
    14821 cagaagctag gtcaaatcct ccaaccgaaa aaatattttt agatactatc aaatccatga
    14881 cagattttat attttcttca gttaaatcat cctttggatt ttctatagaa aaagaaactt
    14941 gtttatcctt tacattttta aagctcatga ctaatttttt cttaatttcc atataattac
    15001 ctccgatata ataatttata ttttaattgt ttgcttgtta atattggtat tttaattgtc
    15061 aatattagtg ttttgcttgt tagtattaat atttcaattg tcaatattaa tgttttgctt
    15121 gttagtatta atatttcaat tgtcaatatt agtgttttgt ttgttagtat cagtgtttca
    15181 attgtcaata ttagtgtttt gcttgttagt attgatattt caattgtcaa tattagtgtt
    15241 ttgcttgtta gtattgatat ttcaattgtc aatattaatg ttttgcttgt tagtattaat
    15301 atttcaattg tcaatattag tgttttgctt gttagtatta gtatttcaat tgtcaatatt
    15361 agtgttttgc ttgttagtat tagtatttca attgtcaata ttaatgtttt gcttgttagt
    15421 attagtattt caattgtcaa tattaatgtt ttgcttgtta gtattagtat ttcaattgtc
    15481 aatattagtg ttttgcttgt tagtattagt atttcaattg tcaatattag tgttttgctt
    15541 gttagtatca gtgtttcaat tgtcaatatt agtattttaa ttgttgactc tagttattgc
    15601 tgatatagaa aatatttaat ttatttcaat taattagatt aaagtagggt agcatctaaa
    15661 atagctaaca tgtttaagtt gaaatacgtt taatatatta atttttcaaa gcgttaatat
    15721 attaaagtta taaccaaaat tgaagttata atctgccaaa tctatagcgt attaagtacg
    15781 ttatataact taatctttta tataaagagt taatttttta atttgtaaag cagagtacgt
    15841 ttaaaagata ctatttagat agtgatgtac tatctaattt taaaatatct acaatagggt
    15901 tctcttgaag acttgctatt gcttctgcaa cagcataaac atcttcattt aatgcctctg
    15961 cttttacatt tgcaaatgat ttacttttaa caactgcctt acccttatca tcaaaaccag
    16021 catccaattt tagttttata ctagataaat tttttgttga aacaacttcc ataaaattac
    16081 ctcctaaaaa tttaatttca aagatttgtc gactttcttt gttagctata atactatcat
    16141 ctaagataat atcggagtta aacagtaatt tctaaagatt ttttgtgtaa atttttatag
    16201 gaaaaatttt acttttttgc tgatgaaaaa gtttacaagt tatttgtgaa gatatgttct
    16261 tagtgttttg aaatagtatt tgtatatatt cctgcttagt tgagatagtt ttgtatggat
    16321 tttcatatgt gtagagttaa atgttaggta ctaaagttta tgttaaatta aatgttaaag
    16381 cttatgttaa aaattttatt aaatttaatc tgtaaataaa aaagttaaaa gtccaatatt
    16441 aacattttac ttttaaacca ttaaaatctt tattaaagct aacaaagtta taagttatga
    16501 gaaattttat tgaagttaat acaaaaatta attttaaagc taagaaatta tttattagat
    16561 tcaataaaac taataaatat agtttagtca ataatgtatt aaataaaaac tttgttaagt
    16621 ctataaaaat atcttaaagg aggtgtaact tatgaagata aaagtggagg atataccagt
    16681 tcaatttcat gcaatggtag aaataattgg aattgataag tttatagagg ttgccaagtt
    16741 gtatggagga acaaatactt atatacctac gtataagggg ctttttagat atgctagaaa
    16801 tagggatata gtaaaacaat ttaatggagt caaccacaat gaattggcta ttagatataa
    16861 tatgtgtgta agcaatatta agcgaatact caatgaaaat agtgtttaaa tataataaaa
    16921 taatgtttaa acataatgaa aatagccttg ttaaacgtaa gagtaaagac aaagaagatg
    16981 ttttataaag tttttttaga caaattaact aaatttacta cttattaatt taaatacatt
    17041 gtatattcta taaaaaaatt tgttaaatac tggagatata ttgagaatac aaagaaaatg
    17101 gtcgaaaata tttgttggct aaaaaataga taaaatgata caattgcttg tataaagggg
    17161 aaaaataaac aaataaacta tataaataaa gaaatagcaa ttaatatgac ataatatatt
    17221 tataaaatta agaatataaa tagatatttt aaggtattag ttgttaaatt aggaaatatt
    17281 aaaaaaaata tatatttata tttaaactat tttgttattt atataaaaat acttatctag
    17341 ttactagcaa ttattagtag aaatggggga aatagagatg aataagggat tttacaaatt
    17401 aaattcagtg gtattgaact ttgatattaa gaatgatgtt aacaagataa gatttaatca
    17461 ttatataatg agaaaagaaa acatacaaag acttcaagga actctgccaa acggacaatt
    17521 ttatatgaca gtgagaagag ctgctacaga tttacaactt tctatttcta ctataagtag
    17581 gcttgtaaaa gagtttttgg atttagggat aataagatta gtgtctaagg gtgtaaaagg
    17641 taatcgttct gtatatagtt acgtaagtat agagtttaat ggaagtttat ttgatgatag
    17701 taaatatatg gaacttgact gtgtatgtaa tgatgaattt attgataata gtggcataga
    17761 gtttgattgt gatagcaaag ataattcttt taatggagat ataaatgtag aaactgtgca
    17821 ttgcattaat gagtatttct ctgatgatga tgtagagcat aattataaca atttctataa
    17881 caaagatact atgaaggata aagccatttc taatattagc actatcaaaa aatatattga
    17941 tgaatctttt gatacaaata aatcagaaaa ttctaactta caacaggagt caataaattt
    18001 caactttaat gatacatgca atgaaataga taaaagaacg aataatagta tagaaaataa
    18061 tcaaaataaa aggcctatga gattgagtga catcagtgtt ttaagtgata aaggtatgac
    18121 aaataaaaag aattacttta atcataatca tggtatacaa aaagggacta aatctgtaac
    18181 aaagaaaaaa gaatccttaa ataaatttaa taaaaaaata tacagcaaag cattataaat
    18241 atgctaaata aaaaatcagg taagaatttt aaaatcgatt ctccaataag cataaaactt
    18301 atcaatgaaa ggctaaagga aggctatgtc ttagaagatt tctacaaagt tattgaggta
    18361 aaagtggcta agtggaaaaa taccataatg gaaatgtata taagaccaga aaccttgttt
    18421 agctataagt ttgaatccta tgtaaatgaa aacatatcta tgaaaagtca aagtaatgtt
    18481 cacccatgta aagaaggcaa tagtaatgat agcagtaatt atatacattc tgattataca
    18541 acatatgatg gcagtgtgcg tgagagaaaa aaatatgaga atgtaagata ctggaatcct
    18601 atatgtgaaa tttaattagt ttaagaatgt atttatgata aataagatat taatagagaa
    18661 tagtgagcta tagcaaatga tatatcaata gttggtagta agttatatta agcaagatat
    18721 tactatcagg tagtgaattg tattgaacga
//
LOCUS       DAENCV010000070        18748 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAENCV010000070 DAENCV010000000
VERSION     DAENCV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.071"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            212..1429
                     /locus_tag="KQD82_002444"
     CDS             212..1429
                     /locus_tag="KQD82_002444"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG4380910.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1524..1904
                     /locus_tag="KQD82_002445"
     CDS             1524..1904
                     /locus_tag="KQD82_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG4380911.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2188..2670
                     /locus_tag="KQD82_002446"
     CDS             2188..2670
                     /locus_tag="KQD82_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4380912.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2691..3506
                     /locus_tag="KQD82_002447"
     CDS             2691..3506
                     /locus_tag="KQD82_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4380913.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3559..5109
                     /locus_tag="KQD82_002448"
     CDS             3559..5109
                     /locus_tag="KQD82_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4380914.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5206..6558
                     /locus_tag="KQD82_002449"
     CDS             5206..6558
                     /locus_tag="KQD82_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG4380915.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGLHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6689..7447
                     /locus_tag="KQD82_002450"
     CDS             6689..7447
                     /locus_tag="KQD82_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380916.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7494..8213
                     /locus_tag="KQD82_002451"
     CDS             7494..8213
                     /locus_tag="KQD82_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG4380917.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8337..9737)
                     /locus_tag="KQD82_002452"
     CDS             complement(8337..9737)
                     /locus_tag="KQD82_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG4380918.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9741..10649)
                     /locus_tag="KQD82_002453"
     CDS             complement(9741..10649)
                     /locus_tag="KQD82_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4380919.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10910..11557
                     /locus_tag="KQD82_002454"
     CDS             10910..11557
                     /locus_tag="KQD82_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG4380920.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11882..14521
                     /locus_tag="KQD82_002455"
     CDS             11882..14521
                     /locus_tag="KQD82_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG4380921.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14579..15682)
                     /locus_tag="KQD82_002456"
     CDS             complement(14579..15682)
                     /locus_tag="KQD82_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG4380922.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16042..16863
                     /locus_tag="KQD82_002457"
     CDS             16042..16863
                     /locus_tag="KQD82_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4380923.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16942..17153
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17240..18556
                     /gene="argH"
                     /locus_tag="KQD82_002458"
     CDS             17240..18556
                     /gene="argH"
                     /locus_tag="KQD82_002458"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG4380924.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7173 a         2154 c         3311 g         6110 t
ORIGIN      
        1 agaatgtaaa aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata
       61 tgtttattgt atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa
      121 aaatcgttta catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa
      181 acaaaaattg gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtaaa
      241 agaggctaga gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa
      301 acttagcgaa aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg
      361 ttcttttaaa ttaagaggag cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc
      421 aaatggagtt atagcttcaa gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa
      481 aatgactggt ataaaatcta caatagtaat gccagcaact gcgcctttag caaaagttag
      541 tgcaactaaa ggttatggag cagaagtagt tttaaatggt gcggtttatg atgatgcata
      601 tgcaaaagct gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga
      661 taagtatgtt atagcaggac aaggaactat atctcttgaa attttcgaac aattagacaa
      721 taaagttgat actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt
      781 tgcagcaaaa gcattaaatc ctaatgtaaa aatagtcggt gttcaaactg caaatatacc
      841 ttcaatgaaa gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat
      901 agctgatggt atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt
      961 ggttgatgaa atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat
     1021 ggaaagtcaa aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag
     1081 tggtaagtat gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat
     1141 agacataaat acgttatata gaataatagg tgttgcttta gctaaagaag gaagaagata
     1201 ttcatttagt acaattatgg aagataagcc aggaaacttt gctgagttaa ctagaataat
     1261 aagtgagaat ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc
     1321 tctaggaaaa caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag
     1381 aataaagaaa gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta
     1441 tataatatac aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat
     1501 taacgaatta ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag
     1561 cagctttagg accatattct caagctataa aagctggaaa cttgttattt gtatctggac
     1621 aagttcctct tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg
     1681 ctcaatcttt aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg
     1741 tagttaagac tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt
     1801 atgctgaata ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac
     1861 caaaagacgt taaagttgaa atagaagtaa tagctgtatt ataaaaatac aatattttta
     1921 atttgaaaag gctgaaaaac tatattctag tgattcagcc tttttataaa aaaataaatc
     1981 acaataaaga acatgataaa tattgtcgaa ttaaaaaata aaattaaaat aaatattgca
     2041 atatttttga gaaggtttta taatgagtat ataatttgaa acgtgtaact attaaattag
     2101 gcatgagttc ttggatattt ttatcctgag gattcatgcc ttttttgtat tgaaattcaa
     2161 aaatctattt aataaggagg aagtactgtg ttaaattttt tcaagaaaaa taaaagctat
     2221 aagttacatg cagtagtgag tggtaacagc attaatattg agaaagtaaa cgattctgtg
     2281 ttttcaaaaa agttaatggg agatggagtt gcaattattc ctaatagtga tgttgttgta
     2341 gccccttgta atggtaaagt aactgtgctt acagaatcaa agcatgcctt tggtatggtc
     2401 tcagatgagg gtgttgaaat attagtccac ataggaattg atacagttag tttacagggt
     2461 gaggggttta aaaatgaagt gtctcaagga gatactgtta aaaaaggtag tccaataata
     2521 agttttgaaa gagaaaaaat aaattctcag ggaattgatt gtacaacaat aataattgta
     2581 ttaaatcata gcgaattttc agaaataaat tgcatggtag aaaatgaagt tgttgcaggg
     2641 caagatactg ttattgaaat tatgaaataa taggaaggga ttaattcaat atgagagtag
     2701 taaaaatctt taataataat gcattatcta cagtaactaa tgataggaaa gaagctattt
     2761 tactaggtct tggtataggt tttaataaac gtccaggaga taaagttaat gaagataaaa
     2821 tagaaaagat ttattatgtt caagaccaca tgcaaacaaa atttttagaa ttattaaaaa
     2881 atgttagtcc agaagtgatg gatgcttcac aacaaattat aagtcttata ccaggaaatg
     2941 agggaaaatt taataataaa gggatattat ctttagtgga acatattagt tttgcaatag
     3001 atagaatgaa aaataatgtt tttttaccta atttaatgct tttggatata aagatgatgt
     3061 attcaaaaga gtttgaatta ggagtaaaag ctttagaaat tatatataga gtatgtcata
     3121 tccatttgcc tgaagatgaa gcaggttaca tagctttaca ttttgtcaat ttacaatcaa
     3181 atgataattt ggcatatgat actttaaaat ttgtaaaagg tagtattgat ttgattaaag
     3241 aatgttatgg attagaatta gacgaatcga gtttaagtac attacgtttt agaactcatc
     3301 ttaaatttct tgctcaaaga atttttaaga atgaaatttg tcaggatgat aaaatgattg
     3361 aaatgtatga ttatttaatt aacaatcatc ctaaaaataa agaatattta gaaaagttaa
     3421 atgtatatat tgaaaaggag tttaggtata aattagataa acctgaaaaa atttatctac
     3481 tactacacct aacaaaaatc ttataagaat gagatgaatt agtaatagaa atatatataa
     3541 ttttaggggg ggataagatt gaaagaaaaa ctacttggag gactggaaac atttgcaaag
     3601 gcaatggttc agccattaat gtatttatcg agtgcaggaa tattaatggt ggtaggtgta
     3661 ctacttacaa atgatatgtt attaggagta cttccatttt ttaaatggga gccaattcaa
     3721 atatttggaa gcctaattta taactgtata atggctatta ttaataatct tgctgttata
     3781 tttgctatag gtattcctgc tgcactagca aaaaaagaga agcacaaagc agcgttaata
     3841 ggatttatga gttatataat ataccttaca gcttccaaca ctatgttaac atcactaggt
     3901 caactagcaa aagaaagcaa gatgcttgga ctgataggga ctggacaagc tactatttta
     3961 ggtattcaag ttgtagatac aagtgtattt ggtggaatta ttttagggtg tatagctgga
     4021 tatgtattta atcgtacaag caacaaaaca tttaaaggag ctttacagat atactcggga
     4081 gcaaatttta gttttatatg catgttcttt gtatccattt taattggagt tgtgagtaac
     4141 tttatatggc cattagctca acaaggaata gcttctcttg caaatgtcat caaagataca
     4201 ggtacatttg gtttattctt atatggtttt ttagagagaa tattagtacc aactggactt
     4261 catcatttaa tttatacacc atttcagttt tctgatttag gaggagtgct taaagttggt
     4321 gcagatacag tttcaggagc ctatgctata gttatgacag aaatgaattt accagttgag
     4381 cgtttctcag atagtatata ctatatggca actggattta caaagatgtt tggttatata
     4441 ggtataggag ctgcctttat ttatactgca tataagcaaa ataagaagaa ggttaaggca
     4501 acgattattc cactaataat tacagcagta ttagcctctg taacagaacc attagacttt
     4561 atgtttgtat tctgtgcacc acttttattc tttgtacatg cagttatagc tggtcttttt
     4621 atagctttat taaaaatatt tgatgttact gcattatgtg gagggaactt attagtatca
     4681 attataatga atactacagt aggcgtagaa aaaacacact ggccagtaat gatgatatta
     4741 ggacttgcac aaattattgt atattttgta gtatttagtt ttttaattaa gaaatttaat
     4801 tataaaactc caggtcgtga agatgagagt acagctacag gcgaagaaca atcaaaacaa
     4861 cttaatttag atgcagggat agaaaatata attgatgggt taggcggaaa agaaaatatt
     4921 aatacagtag aaaattgcat cactagatta cgtgttaatg taaaagatga aagtaagatt
     4981 aatgaagata taattaattt aacgccaaat agcgggattg taaggaaagg aaaagatata
     5041 caaataattt ttggacttca tgtgcatgag gtaagacgtg cagtagaaga ttttttagaa
     5101 gcttattaaa gatatatatt tatatagcaa gggtaaataa cagctttaag tgctgtttac
     5161 tatttattaa agatacatat aatttataga ggaggaataa ttattatgat tattactatt
     5221 gttggtggag gaagcacatt tactccaggt attgttaagt caattgcatt aagagaaaaa
     5281 gagttagagc ttgaggaaat tcgtttattt gatattgata aaaaacgtca agataaagta
     5341 gctgtagttg tcaactggat actaaaagaa gatgttaaaa cagatgttaa actaactgta
     5401 actaattgtg aaaaagaagc gtatacagat gctacattta tatttgcaca aatgcgtgtt
     5461 ggtaaatatg aaatgcgtga gcaggatgaa aaaattccat tgaaatatgg ttgtgtaggt
     5521 caagaaactt gtggttgtgg agggatggca tacggactgc gtacaataca accaatgata
     5581 aaggttattg atgatgtaga gaaatatgct aaaaaagatt attggatatt aaattactct
     5641 aatcctgctg caatcgtttc tgaagcttgt cgtaagttaa gaccaaatgc aaggattata
     5701 aatatctgtg atatgccaat agctattata gatatgatag cggggtcatt aaatatcaat
     5761 gacgttcata atatacgcta tgattatttt ggtctaaatc attttggctg gtttacttca
     5821 atagattata aacatagaga cttaatggaa gagattaagg aatacataaa agaaaataaa
     5881 atattattac caaaatctta tatagaaaac ttaaataaaa gtcgtagtac aggaaatcgc
     5941 catgctgtaa gtagctggat acatgtttgg gaatctgttt atacaatgtt agaaagcttc
     6001 ccaaaatatc tgccaaacac ttatttaaca tactatcttt taagtaaaga aacagtggaa
     6061 cattcaaaca taaatcgtac tcgtgcaaat gaggttatgg aaagccgtga aaaagactta
     6121 tttgaaggtg tgagacatta cttagaaact ggagaaattt ctgaaaaagc attttatgca
     6181 ggtttacatg gagattggat tagtgatttg gcagtttcaa ttaaaaatga tacacgtagt
     6241 cgttttttag ttataactga aaatcgtgga gctataccaa acatgccata tgatgcaatg
     6301 gtagaaattc cagcatatat tggtaaagat ggtccagaag ttattgctcg aggtccaata
     6361 cctctatttc aacaaggatt aatgatgcaa caacttaaca gtgaaaaatt gatagtagaa
     6421 gcttatatag aaaatagtta tgaaaaagct ttacaagcat tcacattaaa taaaactatt
     6481 ccaagtatga atgttgctaa agaaattttg gatgaaatga tagaagttaa taaggaatac
     6541 tggccagaat taagatagta ggataaaaga aagggttttt atttaatcat tagatatgtt
     6601 ttcagagtct tttatcaaaa tgtattcaca aacatatggc aatatattaa aataaagagt
     6661 gatattttta gaagaatggg gtgatgcaat gaacattata aataaaattt tttggcaaga
     6721 agataggatt ggcatgatta caaataattt agaggcagat acacatagtc actgtatgtt
     6781 acaactgttc ttagggatag aagacagcat tgaaataaca gttaatgaaa aattagttaa
     6841 atgtaattgt atcatagtag ataaaaatat ctctcattca ttttcagcaa gaaaaaaagt
     6901 ttattactct gctattattg aacctacatc tatatatgca gaacagctta cctcaaaaat
     6961 gaatgatttt ggctactgga tatgtgacaa taatggatta gagaaattac gtcaacaagg
     7021 aacttttttg atagataact ctagtaaaga acaatattta agatttatgg aaatgcttaa
     7081 taactatttg aatataccta taactctaaa acattatgat gacagaatta ctgaattgct
     7141 caatttatta cgtacttgta attgtgataa tcatactatt tcaagttttg cagataaagt
     7201 gtctctatca gcaagtagat tatcacatct gtttaaagaa caaataggga tacctttaaa
     7261 aagctatatc ttatttcatc aaatggagtg tgcttttaga gaattacttt ctggaaaaaa
     7321 tgttaccgaa gcttctatga ctgctggatt cgatactcca tcacactttg cagggacagt
     7381 taagcgaatg atgggaatgc ctgtttcttt atctttgaaa gatagcgagt ttttgaaagt
     7441 atattaaata ttctcgttat ataatgaagt tgtactaaga aaggggcttt gttatgaata
     7501 aagaatattt agaagaaaca aaaatgttaa attataatga accacaactt aagcttttgg
     7561 tatcatcaaa gaattggttg gaattagatg attttcataa aataaaatct atttatgagt
     7621 ttgtacagaa tgatatttta tttggatata atgcttctga tatgttaagc gcaacacagg
     7681 tattaaatga tggtatggga caatgcaata caaaagctac tcttttaatg gcattattac
     7741 gtgcagtgaa tatcccctgt aggcttcatg cttttgatgt aacaaaagat tttcaacgag
     7801 gagctacatc caaattgatt tcactattag caccaaaata tattcttcat acttgggtag
     7861 aggtatttta tcaagatagg tggattgctc tggagggtgt cataactgat aaaaaatatt
     7921 tagaagcaat tcagaagaaa ttttttaatc atggtggaac gtttaaaaag tatgcaatag
     7981 ctacaaatga tttgaaaaat acatctattg attgggatgg taaagatact tttatccaaa
     8041 aagaagctat cgtgtatgat tatggtattt ttccatcacc agacgtattt ttttctactc
     8101 attcacaaca tatgtcaaaa ttaaaaaatt tcatatatgt acatcttatc agaaaaataa
     8161 tgacaaaaaa tgtatgtaag gctagaaaca attatattga taaaaatgaa tgagtgttta
     8221 agaggtaact atataggaaa tatatagaat aattaaaact gtactggtta aaatgaataa
     8281 aaaggagtta tcacagagca aacttaaatt gttcatagga taactccttt tctgtattaa
     8341 cttctaataa tttgatgtct ttttataaaa catatagata caattaaacc agtaaatgca
     8401 agagtagctg ctattgaaaa tgcaacatct attccatgaa tgttagacag catgtttgaa
     8461 ctcatatgag ctttcttagt agcacttgtc attatagtaa taagtatagc agaacctata
     8521 gctccagata tctgacggag tgtgttatta attgctgtgg catgagatat aagttctcta
     8581 tctaatgttt taattcccca tgtagttaat ggcattaaaa ccattgaaat accaatcata
     8641 cgaatacaat acatcaatga cacatgaatt aaagatgtat ctctgcctaa aaaagcgaag
     8701 gataaagttc ctaaaaacaa acatcctgtt cctaatattg atagagcatg aggtccatat
     8761 ttatctaaat gacgtcctgc aattggattg agtacaacca tcaatatagc accaggtaac
     8821 attaatgaac ctgaagataa tgcagaaaat cctcttacag attgaacgta cagaggaatc
     8881 attaatgtag cagacatcat tgaggcatat acaatcataa ttaatattgt agaaataaga
     8941 aatggtttat ttttaaaaac cctcaattct aaaaaaggtt ctttagattt taattgacgc
     9001 aatgtaaata aaattaatga cataattcct attaatattg gtaaatatgt ggcaatatta
     9061 gtccacccat aattaccttg attagtaaat ccaattaata aaccaccaaa acctagagat
     9121 gatagtacta ttgaaggtat atctaatttt agtttttgag tttccccaac atttcttaat
     9181 aaaataaatg atagtataac atctaatata gcaatagggg aaagaaacag aaataaacta
     9241 tgccaaccaa atgaatctac taaccatcct gataaagttg gacctattgc tggagcaaat
     9301 cctactgtaa taccaactaa actcatagct gcaccacgtt tttcctcagg gcataaatag
     9361 agtataataa cttgtaaaag tggcattaga gaaccagaac ccattgcctg caacacacga
     9421 gcaatcaaca tagttgaaaa attattagaa aaaagtgcaa taatacatcc tatagaaaag
     9481 atagacattg atgtaataaa taactttcta gtactaaatc gatttattaa ataagctgtt
     9541 gtgggtacaa taatacctgc aataagtaga taaattgttg ttaaccactg accaacatca
     9601 gcagtaattt taaaatctga cattatatta ggtaatgctg ttgacaatag tgtttgactg
     9661 agagaactta taaaagcacc agtcattaca attgctatta ttaaatttcc ttttttattg
     9721 ttactttggg tttccataat ttataacgtc tcctttttta attgattttt aatgctgttc
     9781 aataaaagta cttttggtct taaagtgtct tctttttcta aatatgcaat cattgcatcc
     9841 ataattaaag aatcatattg ttgatttttt ccctcgacgt ataatttttt tattgcttct
     9901 agtaatttag gattttcaag gagagaagta tccgttttga ttagttcttc tatatcttgt
     9961 atcgttttaa aaaatagcga tttattatga agggaaaagt aaacttcatc ttcttgttta
    10021 tgtaaaaaca ttgatttttc aattaaagga tttagattat ccatattttc tacaactaaa
    10081 tctagaattt gtatagtaaa tttggcaata aagtatacat ttatttctct tttttgtaca
    10141 tgaataatgt atataaactg atgaattaga gcatccaata agaaaactaa atcaaatata
    10201 attttttcta ttttatcacc atagttatca aataagctta gtttgtattc attcaataag
    10261 ttagctcttg attttgtttg caatttactt gtagccttac gttgctcttc agaaaagttt
    10321 tctcttatat aggtatttat ggtagttcta gaaaaaatat ttttccatac agcaattatc
    10381 tttctgttta acttttctga tgatggaatg tcattatttt catttatgga atgtataatt
    10441 tctaaaaact tttcatcata ataattgatt atttcgcaga taaaactttc cttagtttca
    10501 aaatgcttgt aaaaagtagc tttagacatt ttacactctt ttacaacatc cgatattgaa
    10561 gtattttgaa aacctttttg gtcaaaaatt ttttgtgcaa tagttataat gagcctttgt
    10621 ttttctgtca ctttattatc tcctttcacg caaaaacgtt tagtactata tagtacctac
    10681 aagttttgat aaatatatta tacatcattt ggaaaaaaaa tcaatatata gactgtaata
    10741 tgaaattttt gaaaaatttg ttgttatttt tagtcgtata aaaaagaaac ttaagagttt
    10801 cttaagaatt gtttaaagaa gttgcaataa attagtggta tgattatagt atgaaagaga
    10861 atgtcttgaa atttttctgt atcaaattcc aattatggag gtgtagtata tgaaaaagaa
    10921 aatttgtgta gcaataattt ttgctctatt tatagttttt gtagaaatgt ttattgatag
    10981 ctctagcgaa ggaagtaaaa ttatgacagc tgaattaagt aaatacagga aaggcgtaaa
    11041 tgtattatca ataaacaata ttatagtagc gaataaaaaa tatagtttgc ccaaagatta
    11101 ttctcctcag gaaagcagtg aagcgagaga tgctttttat aaaatgaata aagatgctca
    11161 aaagtcagga ttaaatttaa aagcttttag tacatataga agttatgaat atcaagatag
    11221 attatttaag tcatatgtaa aagaacatgg agaaaaagaa gcaaatagat tttcagctaa
    11281 acctggagag agtgagcatc aaactggact tgcttttgat attggaggag atgaccagtc
    11341 ttgttgggca aataaaaagt tcaacaatac aaaagaagct aagtggttgt atgaaaatgc
    11401 atataaatat ggttttatac ttagatatcc agaaggtaaa gagaatataa caggatatat
    11461 gtatgaatct tggcactata gatatgttgg aactgaacat agcaaaaact ttgcaatgaa
    11521 taatttgact ttagaagaat atttacatat aaattaagag atggtttgtt aaaattaggt
    11581 taaaataaaa aataacctag actattagct gtgtaaagtg atataatgta aatacaattt
    11641 aagataacta ctcgaagggg agtagcttac acgaaagtgg taattaatcg tcaagacagt
    11701 agaaatactc ggttaattag caagggattc cttgatagca agaccttcaa aataagcatt
    11761 aggtatgttt gttttggggt ctttttttat tcaaggaatt tttatttaaa aaaatttaaa
    11821 tttcaagtat acataagagc ccccaaacat aaaataaatt tattgaagag gagggcaaca
    11881 aatgtcttac aaaagtactt atcaagaagt aattaaaaat ttagactcaa gtttatctgg
    11941 tttgagtaaa gaaagagcag aagagttgtt agaaaaaaat ggagctaatg aattaaaaga
    12001 agcagataag gttcctactt ataagctttt cctagaaagt tttaaagacc cattagtaat
    12061 aattctattg atagctgctt tagtacaaat atttttaggt gaaacagtgg agtctataat
    12121 aatttttgca gttattatta taaactctgt tctgggtgtt gtacaaacaa aaaaagcaga
    12181 aagttcatta gaaagtttga aaaatttatc tgcaccaaac gcaaaggtaa taagagacaa
    12241 taaaaaaatg acaattccag caaaaaattt agtagttgga gatattgtat tcttagaagc
    12301 tggcgattac ataccagcag atggaaggct tatagaagca caatctctca aggttgtaga
    12361 agggatgtta actggagaat cagaaccagt tttaaagcat acagaaaaaa taagtgaaga
    12421 tgtaagttta ggtgaccaga aaaatatggt atttagtggt gctgtagttg tttatggtag
    12481 aggttctttt gtagtgactg gaacaggtat ggatactgag atgggtaaaa tagctgggct
    12541 tttagaaagt gctgaaaata aacttacacc attgcaacaa aaaatagagg attttagtaa
    12601 aaaacttggt gtaggtattg taatacttgc tcttttaata tttataattc aagtagcaag
    12661 aaattacttt atggctggaa atggagaaat gacacaaatt attttagatt cattcatgtt
    12721 ctctgtagca gtagcagtag cagctatacc agaagctcta tcatcaatag tcactatagt
    12781 attggctgtt ggaacaaata gtatggcaaa aaaacaagcc ataataagaa aattaccagc
    12841 agtagaaact ttaggttcaa caagtgtaat atgtactgat aaaacaggta ctcttacaca
    12901 aaataaaatg acagtagttg attattatat gtatggaact aataatgatg agttagataa
    12961 agaaaaagta aactattctt atcatgctaa attattgact atggtttcta cattgtgtaa
    13021 tgactctcat ataactagtg atggtaaaga agttggtgac ccaacagaag tggctttaat
    13081 aaattattca agtaaaaatg atttagatta tgataaatta agaagtaagt atatcagaat
    13141 aaatgaaata ccatttgact cagacagaaa gcttatgtct actgtaaata gtatatatgg
    13201 agattatata atgtttacaa aaggtgctcc agatattgtg tttagtagat gtaaatatgc
    13261 tttgaaaaat ggttcaaaag tagatataac agatgagata atagaagaat ataaacataa
    13321 aaatgaagag ttctcaaata gagcacttag agtattagct tttgcaataa aagatgtccc
    13381 agaagaagat tttatacctt caattgatga cgaacatgac atgacattgg ttggaataat
    13441 ggcaatgata gacccaccaa gagaagaagt aatggatgca gtaaaagagg caaagagtgc
    13501 tggtataaaa actgttatga taactggtga ccataaaaca acagcagcag caattgcaaa
    13561 agagattggt ataatggaag aaggagactt ggctcttact ggaaaagagc ttgatgcttt
    13621 gagtgaagaa caactatatg aaaaactaga aaatataagt gtatatgcca gagtctcgcc
    13681 agaaaataaa attcgtatag taaaagcatg gcaacataag aataatataa cagcaatgac
    13741 tggtgatggt gttaatgatg cacctgcatt aaaacaagca gatataggaa tcggtatggg
    13801 aagtggtact gatgtagcaa aagatgcttc tgctatggta ttggttgatg ataactttgc
    13861 aagtatagta aatgctgttg aagttggtag aacagtatac agtaatataa taaaatcaat
    13921 tacttatcta tttgcaggaa acttaggagc tatagtatcc atagtatttg cagtgtttgc
    13981 aggttggtca aatccattta cagctttaca acttttgttt ataaacttag taaatgactc
    14041 tttaccagca attgcattag ggtttgaaaa gtcagaaaaa aatataatga ataaacctcc
    14101 tagacacccg gatgaaagta tactagcagg aggaacattg aaggcagttg ctataagagg
    14161 ttgtataata ggtgtagtta caataattgc acaatatata ggattacaag tatctccaga
    14221 acttggagta gcaatggcat tttcaacatt gatattagct agaataatgc aaacattacc
    14281 tgcacgttct aataatcaaa cagtaataaa attaggtttt ttcagcaata aatacgttat
    14341 aggagcagtt ttagtctgtc ttgggcttta ctctttaaca ctaataccag tgctaagagg
    14401 aatattctct ataccaacaa cgtttggatt gtaccagttg gggatttgcc tagggttagc
    14461 attaatttca tcaatattaa tggaacttaa caaattaata aatagaagtg aagtaaaata
    14521 atcaaaaaat taaagggcat attgatttta acaatatgcc ttttataaat agtctaattt
    14581 attctatatt atcgattatt tggaatgctt ttataacttc aggtaattct ttttcttcag
    14641 gcattctaaa tcttatgaaa tttttatcaa gtccagtaaa atcacttcca gatataactc
    14701 ttatttttaa tttagcaaac tcttgttgta aatctatgtc cttgttttta tgttcaactg
    14761 tcataattga tacagtatca ctagtttcag ctatattaag gtttttccat ggaattaata
    14821 attgattttt tatatctttt gttttttctt ttaattcttc tataaattga acatcatcta
    14881 ataaatttgc tgcaatacta cgagatattt cagacacttc gtaaggagta gaaatctttt
    14941 taatagggct aacaagttgt tctggaagaa ctgcatatcc tgctcttagt cctgctaaac
    15001 caaatccttt tgaaaatgtt tttaaagcaa ttacattatc ataattattt agaagcttga
    15061 cagctgaatt ttcttttggc atgtattcac cataagcttc atctaccata actgctatat
    15121 catatttagc tgcttccttt actatatttt caattgatga taaaggtata atttgtcctg
    15181 taggattgtt tggattgtct atgtaaatta ctttatattc tggatttatt ttttcaataa
    15241 attctttttc attaaacttg aaattatcct ctttctttaa aagaacataa tcataagttg
    15301 caccatgcat ttttatatct gtctcatatt ctgagaactg aggtgaatat cctaaaactt
    15361 tatctccctt ttcaataaat agtctattta aaaggtaaat aacatgtata gaaccatctg
    15421 ccaaacaaat tctgtcagta tctaatgcta taaaatcttt ccaataatcg actatattat
    15481 cttttaaagc aattgaatga ggatatcctc ttatcatatc aaattttaaa gcattaaaag
    15541 cttctacagc tttgtttgag taagacacgg tatttatacc ttcaccacag tctatctcga
    15601 atacatcttc tgaatgtcca gattctattg cataactttt tttcaactgc atttcaatag
    15661 ctttgttaat ttttagtttc atactttatc actccttatt tagtcaataa taaatgttat
    15721 aaaagttgct agatttaaaa tattatgaaa atatataata gtaaaaatga atataaatat
    15781 tataatagct cactttattt aatatgatta tatatcatat attgtaaaat ttctagcatt
    15841 ataagactat aaattgaaaa tattcagaaa aatactacaa gaaacgattt attttgctct
    15901 taaaataagt taaatgaatg ttattaattt tgtaaattga aatagtttaa gtataaagtt
    15961 atgatataat ctaataaggg taattcttta gattatatta aggaaatata ggtatacaat
    16021 aaaaagttat ggagttggat tatgggatat aaattaattg taacagatat ggatggaact
    16081 ttattaggca ataatcataa agttacagat gaaaataaaa ctgcactaca aaaagttata
    16141 aaaagcggta ttaatgtaac tttagcaaca ggaagagcct tcgattcagc taaatgcaat
    16201 gtagattttc ttaaagagga catgccaata atagcttgta atggttcatt aataagagag
    16261 caaaatggaa atataatata ttcaaataag attgatacaa gaacatgttt aaacatttta
    16321 gatgtattag ataaatacga tatttactac caatgtaata gtatagattc tatgctttcg
    16381 aaaaaaatag aaggacgtga agatagatta agtgtatttt taggctctga aacagaagta
    16441 attgtaaaag atgatttgag agaagaaatc tttaaaaaag atatactaaa atttgtaatc
    16501 atagaagaaa aaaatccatc tattttggat gaaataagaa aagaattgag aaaagttcaa
    16561 ggaataaaga taacttcttc atggccaaac aatattgaag tcatgaatga aggtgttgat
    16621 aaaggtaatg cagttaaaat tttagctgaa aaaatgaata tagacagaga agatataatt
    16681 gcatttggag acaattacaa tgacatagaa atgataaagt ttgctggtct tggggtagct
    16741 atgggaaatg cagaagaatt gattaaacaa gaagctgatt atgttacaga tacaaatcaa
    16801 gacagtggag ttgcaaaggc aatttataaa ttcctagagt taaaagaatc acttagctct
    16861 taaaacagta tataatagga aaaaataggt tgactatttt gtagatagta attataatac
    16921 aaatagataa aacataaaaa tttagattca gtgaaaaaga gagtagatat agagaaaatc
    16981 tcagcgaatt gggggatggt gtgagcctaa tgataacact ttatattgaa aagaactttg
    17041 gagaatacta cttgaaattc agtatggtag taggtaatct cgccttaaga gaatagagtg
    17101 ggtaaacatt gtttaccaac tagagtggta acacggaata tttcgtctct tggtcttttt
    17161 gaccaagaga ttttttagtt tcattgagtt acaagactta taattaaaat acaaaaaatt
    17221 aaaatatgag gtgaaagtta tgaaattatg gggcggacgc tttagaaaag cggaaaatca
    17281 acttatggaa gaatttaata aatcttttgg ctatgattgt gtactgtaca aaaaagatat
    17341 cgaaggaagt gtagcacatg ttcatatgca agtaaaatgt ggacttttaa cagaagaaga
    17401 aggaaagtct ataacagaag gtttaaaagg gatacttgaa gatgtagaaa atggaaagtt
    17461 agtattggat ggagagtatg aagatataca tagttttact gagataaatt taattcaaag
    17521 aattggagat gttggcaaga agcttcatac agcacgtagt agaaatgacc aagttgcagt
    17581 tgatatgaga ttgtatgcta aagaaaaagc aaatgatttg gtggatttaa tatctgaatt
    17641 taaagctact ataaaagatg ttgctgacaa aaacccagtg atgatgccag gctatactca
    17701 tcttcaaagg gcacaagtag tgacatttaa gcatcactta atggcatatt atagtatgtt
    17761 tgatagagat gagaaaagaa taaaaaatgc tatagaaata ttagatgaat cacctttagg
    17821 ctgtggagct ttagcaggaa ctacgcatga tatagataga agtataactt gtgaacaatt
    17881 aggatttaaa aaagtagtag ataattttat ggatggagta agtgatagag attatttact
    17941 agagctaatg agtgattttt caattataat gatgcactta agtagattaa gtgaagagtt
    18001 gatactttgg agttctcaag aatttggatt tgttgagata gatgatttat atacaactgg
    18061 aagtagtata atgcctcaga agaaaaatcc agatggtgca gagcttataa gaggtaagac
    18121 tggtagagta tatggaaact tatttggtct ttttacagta atgaaaggaa taccattagc
    18181 atataataaa gacatgcaag aagataaaga aggttttttc gatagtgtac atactcttga
    18241 aatgtgtata caaataatgg atagaatgat agccacacta aaggtaaatg aggataaaat
    18301 gaaacaagca gtaaaaaatg gttttttaaa tgcaacagaa gttgcggatt atttggttaa
    18361 aaataatgtt gcttttagag atgcacatgg tatagtagga agtatcgtta tatattgtga
    18421 agataataaa aaagcaatag aagatttaac attagaagaa ttacataagt tttcagatgc
    18481 ttttaaggaa gatatttatg attttatcga ctatgagagt atattaaata agggaataaa
    18541 aaagaattta aaataatgta tttattttta aaagctgtaa atttgaatgt tatcatttaa
    18601 atttacagct ttttattgtg taagatttta ttatataaaa gtaagaaact taaaaggagt
    18661 agtatttaaa ataaatacaa ataaaaaatt attattaaaa tgataattaa tatcaataag
    18721 tattgacaaa attatttcta aatactat
//
LOCUS       DAENCV010000071        18599 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAENCV010000071 DAENCV010000000
VERSION     DAENCV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.072"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            281..1441
                     /locus_tag="KQD82_002459"
     CDS             281..1441
                     /locus_tag="KQD82_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG4380925.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1783..1944
                     /locus_tag="KQD82_002460"
     CDS             1783..1944
                     /locus_tag="KQD82_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380926.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2137..2766
                     /locus_tag="KQD82_002461"
     CDS             2137..2766
                     /locus_tag="KQD82_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG4380927.1"
                     /translation="MKIVTYNIHKCMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            3005..4264
                     /locus_tag="KQD82_002462"
     CDS             3005..4264
                     /locus_tag="KQD82_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG4380928.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4523..5518
                     /locus_tag="KQD82_002463"
     CDS             4523..5518
                     /locus_tag="KQD82_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG4380929.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5764..6831
                     /locus_tag="KQD82_002464"
     CDS             5764..6831
                     /locus_tag="KQD82_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG4380930.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7365..9275
                     /locus_tag="KQD82_002465"
     CDS             7365..9275
                     /locus_tag="KQD82_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG4380931.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9338..9961)
                     /gene="ytaF"
                     /locus_tag="KQD82_002466"
     CDS             complement(9338..9961)
                     /gene="ytaF"
                     /locus_tag="KQD82_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG4380932.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10149..10793
                     /locus_tag="KQD82_002467"
     CDS             10149..10793
                     /locus_tag="KQD82_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4380933.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            11007..12065
                     /locus_tag="KQD82_002468"
     CDS             11007..12065
                     /locus_tag="KQD82_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG4380934.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12169..13353
                     /locus_tag="KQD82_002469"
     CDS             12169..13353
                     /locus_tag="KQD82_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG4380935.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKGDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13560..14273
                     /gene="rpsB"
                     /locus_tag="KQD82_002470"
     CDS             13560..14273
                     /gene="rpsB"
                     /locus_tag="KQD82_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG4380936.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14352..15263
                     /locus_tag="KQD82_002471"
     CDS             14352..15263
                     /locus_tag="KQD82_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG4380937.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15348..16052
                     /locus_tag="KQD82_002472"
     CDS             15348..16052
                     /locus_tag="KQD82_002472"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG4380938.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16078..16635
                     /gene="frr"
                     /locus_tag="KQD82_002473"
     CDS             16078..16635
                     /gene="frr"
                     /locus_tag="KQD82_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG4380939.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16638..16841
                     /locus_tag="KQD82_002474"
     CDS             16638..16841
                     /locus_tag="KQD82_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380940.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16870..17604
                     /locus_tag="KQD82_002475"
     CDS             16870..17604
                     /locus_tag="KQD82_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG4380941.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17617..18396
                     /locus_tag="KQD82_002476"
     CDS             17617..18396
                     /locus_tag="KQD82_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG4380942.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7652 a         1854 c         3230 g         5863 t
ORIGIN      
        1 caaaataaat aaataataaa atatttagtt taattatctt tacaaataaa aattttagta
       61 ataaaataat tgttgaaaaa gattttaaat tcagattatt catttctaaa gtaattaatg
      121 tgcaaatctt tattaatata tgtcaatagt taggtatata tgtatttaat tagtatgata
      181 tagataaaag tatttatttt aattgtctat tatctgaaaa agtctttact tagaaatcat
      241 aaaaatcaaa ttaattagga aaagaaaggg tgtgtataat gtgattgaga ctggatttgt
      301 atattgtagt ttcttggttg tattcgtagg tgtaattttt tttatggttg ataaattgaa
      361 atggaaggta tttgatgtta ttaatccaat gcttttaata tacttaggtt cagctatact
      421 tggatcattt ggtttatttg cacagaatga agatgttgga acgtatcaaa acataataac
      481 agtgaacttc cttccattaa tgttagtgtt tttgttaatt caatgtgatt taagaaaaat
      541 attaaagatg gggcctaaaa tgttattatc atttttctgt gctacattta catttatgct
      601 tggatttgtt atagcattca taatttttaa aaattcaatg ggtgcagatt catggaaaat
      661 tcttggggca gcatctggtt catggatagg tggctcatca aatatgattg cagcagctaa
      721 tgctctagga gttgcagaag aaggacttag ttatgcaata cttatgggtt ctataggata
      781 tacagtatgg atgtctgttt gtctaatgtc tgtgaaattt ggacctaaat ttaacaaatg
      841 gacaaagtgc gatacaagtt ttatagatga ggttacatca aaacttgaaa aagaaaataa
      901 agataatacg gcacctacgt ttgtagaact tatgatttta ctttctgttg ggtttggagt
      961 tgcgtctttt gcacaaattg tatccaagat acttccaact tcaggtatac ttaatgaaac
     1021 tatgtgggta gtacttgttg catctacagt atcaatatta ttgggtatga caaaaatatc
     1081 aaagcttaaa gggagtacac tggtaggaaa tgtgtttatg tatttactcc ttgcagctac
     1141 aggtagtaaa gcaaatttct ttggattgtc agaagcacct gtttacataa tgtttggatt
     1201 ggtagtagtc cttgtacatg ctatttcatt tatagggctt gctaaactat ttaaacttga
     1261 tttatttact tgtgaagtag gaagtatagc taatatagga ggagtaacta cagctccaat
     1321 gattgctggg gttcacaatc ctgtattaat accagtaggt gtacttatgg ggcttctagg
     1381 aaatattata gggacttatt gtgctcttat ggtaactcaa atattacata tgttagctta
     1441 ataaaaatat ggtataaaat taataattta aataagaaaa gtcatttcaa tttaagcaga
     1501 agcttttact gaaatggctt ttttgtttga caagataatc tatttcaaga agatgttttg
     1561 acatatttag tgtataatat tgtaaaattt actaaaaacg agatatattt aatttttata
     1621 ttgaatcgaa cgtctgtatg atgtataata aatatacaga acgaatgttt gcagaacggg
     1681 tgttttttga atttatttta ataatttatg aatatatatc agaatgcata aatatattta
     1741 aatataaaga ttaaaaagaa tttaaaggag tgttgataac ttatgataat tccatcaaga
     1801 agaatacaag atataacatt aacaaattta aaaaatggtg aagtaacttt aattgaattg
     1861 gatgaaatat acaagaagat gggattttta tttgttgtta gtgaaggcaa attgaaaaag
     1921 ataaaaaagg agaatcatca ttagaaatga atgaaataat aaatattata atagtttata
     1981 aaacctaact tatgtagaat ataaatactt aagttaggtt tttttgtgat aaagtttata
     2041 agtactcaat tttataagat aaataatggt cttaagtgaa aagtaaataa gtttaaattt
     2101 tagtaagtat tatattgcat aagaggtgat attattatga aaatagtaac ctacaatatt
     2161 cataaatgta tggattcaaa taatagatta actctaacta aaatggggtt atatttaaaa
     2221 caattggatt gtgatgtaat ttgtttgcaa gaagtattat atcctcaatt tttagcttta
     2281 aaagctgttt taaatatgga tggtgtcttt gcgacaaatg taaaaaaagt aaatatgata
     2341 tatggtattt gtacatttac aacatctaaa atgttaaaca gtaatcattt tttcttaact
     2401 agcaaaaaag aacaacgggg agctctttgt ataactatag atgcatatgg aagaattata
     2461 aatgtaataa atactcactt gggacttgat aggcaggaga gagcaaaaca attagacgaa
     2521 ataatagact ataggaacag attagtgggg atagttattt tatgtggtga ttttaatgag
     2581 aaaaatgtct ttttaagtat gtttaatgat atggctattt cactaaacaa gtcttactta
     2641 ccaacatttg aaaaatcaaa ctctagaata gattacattt ttgttaataa aaatactgaa
     2701 ctaaaaggat acacagtaga aaaaatttac ctgtcagacc attatcctgt aataggttac
     2761 atttaaattt caatataaat gaaattagtt gttataaaat aaacaatttt ttgttaagaa
     2821 atagtaaaag aaatataata aaaaatatca aaaataaaac taattataag acataaattt
     2881 acaataaagt tacatttgat taaattcctt gtttaacaaa agctattcaa atataatcta
     2941 aggtagtttt gaaatgggaa ctctattatg caaatttaag ctagtgttaa aatgaggtga
     3001 agttatgggc agaagaacaa caagagtttc aagattaggt aaagaaaaaa aacgaagtgt
     3061 tttaattgta ttggcagctt tagttctaat aatgggtgga tttacttatt tttttaacag
     3121 taaatttctg tacaatggga aaatcgctaa aaatgtatat atagaaggag taaatgtatc
     3181 tgatatgact aaagcagagg cattaaaagc tataacagat aagtatactc cagaagattt
     3241 aaatcttgct tatgatggga aaaaatatac aatatcacca aaagatattg atttaaaata
     3301 tgatacagaa gatgttgtaa aagatgctta tgaaagtaca aaaaaagggt cctattttca
     3361 aaacttaaaa aaatatattg atataagagt gaataaggca aatatgaaga taaaagctga
     3421 atatgatgag gcaaaactta gttccaaagt gtcttctata gcagatagta taaatgttaa
     3481 gatgaaaaat gcttctatta gtgttggaag tggtggacta agttatacag actctgttgt
     3541 aggtagagaa tttgatttgg cagcaaataa agagtctata tataatatga taaaaaataa
     3601 agagcataaa actttagaac ttaaagtaaa tcttcaaaaa ccagatataa caactgaaca
     3661 agtaaaaact gtaaattctg ttataggtca atattcaact acttattcac aagctgtaga
     3721 gggtaggtca tataatgtag gattatcagc aagaaaaact agtgatgtat tattaatgcc
     3781 aggtgaagaa ttttcataca ataaacttac aggacctagt aataaagcaa atggttacaa
     3841 agatgcacct gttatagttt atggtaagct agaacaatca gcaggaggag gtgtttgtca
     3901 aacatcttca acagtttata atgcggctct tctttcgggt atggagataa ctcaagttac
     3961 aaatcattca tctgcatcta cttatgtgcc taaagggaga gatgctactg tgagcgatgg
     4021 aggattaaac ttaaagttta aaaatccata taagcatcct gtatatatta aaaattatgc
     4081 aggtggagga agtgtatcat cagtaatcta tggtaattct ggtgacaaac caaatatatc
     4141 tatagaggta aaacaaactg gtcagaataa atattctaca tatcgaatat ttaaagattc
     4201 aagtggaaag gtcataaaaa aagaacatat atcaaatagt tcttataaag aattaaaaaa
     4261 atagataatt ttttagagaa atatttgtaa tataaaaggg agtgtctcaa aatgaacttt
     4321 ttagtttatg aggcactctt tttctgtgaa atcaaatata ttttcatcat ttcaacaata
     4381 caaaagaagc gtatctctat tttgagacag ccttttttgt aaaaattaac actattagtt
     4441 cttgttttaa tattacaatc attaatatat agaaaatatt cctatatatt ggtataattg
     4501 taaatagatt tagttatata aaatgtatga ggaggtgaca tggttgaatt ttataggcaa
     4561 tagatatgag gtagtaaatt ctgacgacgt tttagaaatt aacaaaatct ataaagctag
     4621 agatgttttt tacaggaaaa atgtgttaat aaaagttata aagcacaata attatatctg
     4681 tgaagatttt gtttcaaatt taattgatga aagtactgct ttagatgaga taaactctcc
     4741 ttatatacta aaaataatag atgttgggat tcactgtaca gaagaaacaa cattatatta
     4801 tgttgtaagt gaagacttta atggtatcgg actagatgaa ttaattctag gtaattatct
     4861 tcatttagaa gcaattgtaa acataatgat acaagtttta aaagctttag aaatgataca
     4921 taggaatcat tcatatcatg gtagtttaaa atcgagtagt ataatagtag atacagaata
     4981 caatataaaa atttgtgatt ttggaattac aaaagcaaat aatggggtta atacaagaag
     5041 ctatggaaac aggagatatt tatgcccaca tcaactatgt ataaattata ctgataaaga
     5101 aagtgatttt tttgcaacag gtcttattct atttgaatct atatttaaaa aattgccttt
     5161 tggagaatca aattcagaag aaaaaatgct acaatctatt gataagggat tagattggaa
     5221 tagtataaaa gcaattaatg gaaatactga acttataaat gttataaaaa ggcttcttgg
     5281 aagaaataat aagtatacca gagccaatga tattattatt gatttaagta aagtaatgta
     5341 tgaaaaagca tatatagaag ttgaagatga gaaagaagaa gagcagaaac aagacaaagt
     5401 tatacaagtt aaaaaagaat ataaaagtaa aatgagaaaa aaattacata aaaaacttgt
     5461 tgttgccggt ctagctatag tcatgatttc aatgattatt attagttcca taatataaaa
     5521 attgtatatt aagtatttta aaatcaatgt ctataaagga tatacataga aattttagtg
     5581 tagtcttaaa ttatgaaagg aagtggttat tatgttaaca aaaaataatg aggtattaaa
     5641 cgtctaggag tattttaaca aatagccacc tgcttttaca gtttagaata aaaaagctgt
     5701 ggctaaaggt cacagctttt tgctactttt taaaggcagg agaatattaa aaaggagcaa
     5761 attatgagca atataaaaat aatagcaagt aataaatgtt ggattgaaga tatagcaagg
     5821 aaacaacttg aagatatttc aaagttaaat ggaattttaa gagtagtagg attacctgac
     5881 ttacatgcag gtaaaatacc tgtaggttta gcagttgaga ctaaaaatat tatttatcca
     5941 catataattg gaaatgatat aggatgcgga atgactttat ttaaaactgg agttctaaaa
     6001 aagaaattta aaaaagacaa atggattaag tcacttagta agattaaaga tttgtctgat
     6061 atagaaataa aaaacacata taaagaagaa tgtccaattt taaatttagg aactataggg
     6121 agtggaaatc attttgtaga aattcaatgt ataagtgaaa tttataataa agaacaattt
     6181 gaacaattaa aattttcatc tgatgatatt atgatgttag ttcactgtgg gtcaagaaat
     6241 tatggagaag atattttaaa aaaattttat gataaagatg gacttgaagt tgaaagtgag
     6301 aaagctatag attatattaa aaaacatgat aatgctttaa tgtgggcaga gcgtaatcgt
     6361 gaagctatca gtaaaaaaat tatgcagtca ataggtacat ctggagatgt tgaaacaata
     6421 ttctctataa atcataactt tgtagagaaa agggaagata aatttataca tagaaaaggt
     6481 gctgtttcaa gtgaaagagg agctgtgatt ataccaggtt caagaggcag tctatcatat
     6541 atagttatgc ctactgaaaa tacagagata tctttatatt ctctatcaca tggagcaggt
     6601 aggaaatggt ctcgttcagt ttgtaaatct cgccttaaaa gtaagtatag taaagatact
     6661 ataaaacaaa ctaaatttaa aagtcagatt atatgtaatg atgtaaattt attatttcaa
     6721 gaagctccag aggcatataa gaatatagag cagattattg aaagtttgat agaatatgaa
     6781 cttattcaag tagtggcaac tatgaagcca ttgattactt ataaaggata atttttaaag
     6841 ttaaaaaaga ttaaaattat attataaata gctaatttta tggaacattt aaaagtgttt
     6901 tattattagc tattttttta ttttgtagag acgataattt atagagctaa attctttgat
     6961 atataatgat tacaaagtta gactgtattg gaaactgcac atacatgaat tgatgacaat
     7021 ataagtgatt ccaaaatata aatgaaagta taagcttatt ttcactataa aaattttcac
     7081 tgtacaaaaa cattaatgac attattttta ttgacataaa tatataattt atggaataat
     7141 attagaagca ctgatataag tgtttaaata aggatagaaa aggagaagtg ttttatgaaa
     7201 aaaaatacta acattaatga gtccggagtt aaacttctta ataaataagg agtttgatat
     7261 ataataactc cttatttatt aagttgttcg caaaataact attatcaaag taaatgatag
     7321 ggtgatttaa aagacgataa cttataaata aaggagaaaa aataatgaat agtaatacat
     7381 ttgaaagatt acaactaaat gaagttaaag aattaataaa aatacattgt gtaagttcac
     7441 taggaaaaaa tttaatagat aaattaactc ctagtgggaa tattggagta gtaagaagga
     7501 aattagcaga aaataaagaa gctagaaaga taattgaaaa tagtaatcac ataccacttg
     7561 aaggattgtt taatgcaggt tctacaatag ataaaattga aaaaggaatg ataatagaac
     7621 ctgttgaact tgtaaatatt gaagattttt taagaggttg taggaagatg aaagctttta
     7681 tgttagaaaa agaattttat tcacctacat taagttctta tgccttaaat ataacagaat
     7741 gtaaaagtat agaagatgag ataaattact gtataaaatc taataaagtt gattctaatg
     7801 ctagtaaaga attaaaaaag ataagacgaa atatagaaat tacagaagga aagataaaag
     7861 atagattaaa taaatttata acatcaactg taaataaaaa gtatatacaa gagtttataa
     7921 taagtaaaag aaatgataga tatgtaatac caattaaatc ttcttataaa aatgaagtta
     7981 atggtactat attagataca tcatcaaaag gaaatactgt atttattgaa cctattagtg
     8041 tttcaaattt aagtacagag ttaactatgc taaaagctga tgaaactatt gaagaataca
     8101 aaatattatc ttatttaaca gaacttatat ttgagaagat aagtcagata aaattaaata
     8161 tagagatact ttcagaatac gatatggtat ttgcaaaagc taagtatagt caaaaaataa
     8221 aaggaattac tccaaaaata aataataatg gctatataaa aataattaaa ggtaaacatc
     8281 cacttctaac aggagatgca gtacctttag attttgaaat aggcaaaaat tatagaagtc
     8341 ttataataac aggtcctaat gcaggaggaa aaactgtaac ccttaagact gttggtttac
     8401 taactttaat ggttcaatgt ggatttgata tttctgcaaa agaaggtagt gaatttagtg
     8461 tatttgaaaa agtttttgta gatatagggg ataatcaaag tattgaaaat gccttaagta
     8521 ctttttcatc acatataaaa aatattgcag agattatgag tttatcaaat aattctactc
     8581 ttgttctgtt tgatgaaata ggaagtggaa ctgaacctaa tgaaggagct ggtttagcaa
     8641 tatcattatt agaagagttt tacaaaatgg gatgtataac tatagcatca actcattatg
     8701 gagagataaa aaagtttgct acattgcatc cagaatttga aaatgctggt atgatgtttg
     8761 ataaagaaac gttagaacca ttgtataagt taactattgg aaaatctgaa gatagtaatg
     8821 ctctttttat ttctaaaaaa atgggaataa aaaatagagt tttagataga gctaaagaat
     8881 atgtaattga tagaaactac aatttagatt tagttaaaag aagtaagcta caatctaata
     8941 ttgaagataa aaatagttct gaagtcattt taaactatac agattataat gtaggggata
     9001 aggtaaaatt actagatgaa gatgattttg gaattgtata taagcctatg gacaagttta
     9061 ataatgtaga agtattgttt aatgataatt ttatttcagt aaatataaaa agattacaac
     9121 ttagcataaa agcatcagaa ttgtatccag aagggtatga tttagatgta ttatttacta
     9181 gttttaaaga aagaaaatta gaaaaagata ttcaaagagg ttcaaaaaaa gctcttaaaa
     9241 agattcagaa agaaattaga gagaatagaa aataaatata aaaagcacaa aagaaagcaa
     9301 tctaaagaga aaagtttatc tttagattgc tttcttatta acatatcttc ataaatccaa
     9361 tgactaacaa gattaaacca cttaaccaag ataagtttaa atttacgttt ttaacaaaca
     9421 tatttcctag taaacaacca agtaatactg caattatatt tgatattaaa gaaaatagaa
     9481 taacttgtat ataattcaca cctacaaggc cactaccaaa accgatagct aatccatcta
     9541 aacttaaagc aagtgcaagt gtaaatgctt cagcagattt aagcacttta gatttacttg
     9601 tatctaagac atcaggttcg atacttacat taaaatttaa tttaaggtct aacatattaa
     9661 aattataatt ctttgagtgt atagattttt tcttaaaata agatttaaaa aaactttcaa
     9721 ttagtctagc acttcctaat aaaaataaaa taaagaaaca tattaacatt gttatatttt
     9781 gaggtaaaaa atctctaact attgtacctg caaaaagcga tattgctaaa atagctgaac
     9841 aaactacgct gataattata tttgaatata agggaacttt aactttattt acaccatatc
     9901 caaagcttgt cacaaaggca tctatacaaa gagaagtaac taataaaatt gattcaatca
     9961 cagtagatta acatcctttc tcttaaattt tgtacttata ttagttatag tattgtaaaa
    10021 atggataaat tgttacagat aatccaataa attgttgata aaattccaac atcatacaac
    10081 acctaaataa caaaagaatc atatcatata ataatataaa accaatcata ataaagcggg
    10141 tgaaaataat ggaacaaaat aataaggttg ggaatttcgg tgatttgttg aagattttat
    10201 tagatgaaaa agaattatca atgggagaac taagtaaaaa aagtggaatt gataaatcta
    10261 ctatatctcg tatagctaat aataaacaaa agccaaatat aaatcattta gaaaagatgg
    10321 caatacattt aaatattaac ctagaagaac tgttgaaagc ttcaggatat gaatttagaa
    10381 acagcaataa tcaaatattt aatgcagata gtgattttag taactttgat gatattcttg
    10441 gatttgcaaa tttaatcaat gataagaact ttaatggaaa tattgaaaaa gagttaagta
    10501 agtgtaagtt atatgtccaa acagatgaag gtaaaaaatt actttttgat aattttaata
    10561 aaaaaataga ttctattgaa aaacaaggtc aatttacaga tagattaaga caaatgtatg
    10621 ctgatttttg tacagatgga cttacaataa agaaatattt attgattgga agtatgctac
    10681 tttactttgt tatatcaaca gatgtgattc cagacttcgt gttcccaata ggatttatgg
    10741 atgatttagt agcattaaat atagttacaa aattacttaa aaatgataag taagttttag
    10801 aatcatattt aatatataaa taaatcaaag tatataaaat ttattatttt ttatatgaaa
    10861 caaattaaaa gcacaatgaa gtagattaaa aatttaaaac taagaatcta atatatattt
    10921 tgtaactatt taagtatata aaataaaata atatccaaac taatacaaaa tagtaataat
    10981 tgttttgtac tggaggatat atatttatga attgtaaatg gatatctaaa atagatgaga
    11041 gaaaaaaatg tcatagagaa gcagattctt ctggatattg tatctttcat aaagaaaata
    11101 agtctgatga agagatacag ttgatgatgg atactcttca taaagaagag ataagtgaat
    11161 ttaatggatt tgtttttgaa aatgaattta atgcggaaga aatattaact tacaattata
    11221 aaatactgga tttttctgaa tccatattta aacaaaaagc aaattttaaa aaatatattt
    11281 ttaaaaagaa tattattttt aactatacag aatttagaga taaagtttta tttaatggtt
    11341 gtgtattctt agaaaactgt gattttaata gaaccatatt tagtaaacac tatataaatg
    11401 accgtatatt tgaaaaagtg aaatttaaag gacctgattt ggtagttaat aaagtagaaa
    11461 attttccacg aatggatgga ataattttta gcatgtgtac aaaatttgtg ttaaagaatg
    11521 ttgaatatgg aaaaagtgag tatgagcatg gcaagataaa ttatagaata gctagaaatc
    11581 aagcaacaaa aattggagaa tatgagatga taggatttta ctattataag gaaagaattt
    11641 atagtagtaa aataatgaaa cgttctaatt atcctacttt cagtgattat ttagttgaaa
    11701 aattttttga ccaaatagca cgttacacaa caggttatgg agaaaagcca tggaatatat
    11761 tattagttat tatagcaata ataagtgttt ttgctttatt atacttattt gtaggcatag
    11821 aaagttcaaa tagcacactt gtagctttag atataaataa tataggtgat tactcactaa
    11881 gtgagatttt caaaatgtat atggatttat ggtattttag catggctaca tttagtacag
    11941 taggatatgg agatatggtt gctacaagtt tgattgggaa agcattggca ggtatagaag
    12001 tattttttgg agttacaata ggagcaattt gggcatctgt gattattaaa agaatgataa
    12061 ggtaatattt gtgtaaatat ggataggtat taattactag atataaattg gactaaatag
    12121 ggaaaattta aatataaata tacttaataa tccaagaagg tgaaaaattt gaaaaatgca
    12181 ctaaaaaaaa ttatatcatt agccatgatt ctagttttta taataccaaa ttattcatat
    12241 gcagatgata aatctgtaga caagtattca aaaacatcta tacttataga ccaagaaaca
    12301 ggtagagttt tatatagtaa agaacctgat atgaaggtac cattagctag tttaagtaag
    12361 atgatgactt ttttacttgc aatagaagct attgaaaata aagaagtaaa agaaactgat
    12421 aaagtaaaaa tagacaaatc tattgcatca gtaaaagggt caagttataa acttaaagat
    12481 ggtgaagaag tgccacttat agagcttatg agaggtctta tgattgtatc aggaaatgat
    12541 gcggcaattg ctatagcaaa acatatttgt aaaacgaaag aagcatttgt agatagaatg
    12601 aataaaaaag caaaagaaat aggaatgagt aatacacatt ttctaaatcc aaatggtctt
    12661 ccaatatacg atttaagcaa ccccaaaaaa ccagcaaaag aaaatatctc tactgcaaaa
    12721 gatatagcta ttcttggaaa atacatgttt gaccattatg aaaaacaagt aacagctata
    12781 actgatatgg agacatatac aaattcagat agaagttttg aaaaaagcaa tacaaatgca
    12841 ttattaagaa ttataccaga agtagatggt ataaaaactg gatatactgg caatgctgga
    12901 tattgcttat cattttctat ggttgtaaat aaaaatgata aaaatgagaa aaaacgtaga
    12961 gtaatagggg ttgtattagg tactaaccat aaaaataaaa gaacctctgc ttcattagct
    13021 ctactgaaat atggaaaaga aaatttcaat atgaaaaaag taattgataa agatagcttt
    13081 attggtaaga aatatataaa aggaatgaaa gagttagagg ttacattaaa agcaaaagat
    13141 gaactttata caatattaaa aggtgatgaa agtataaaat cagaagttaa gattaaaaat
    13201 atagagtacc ctgttaaaaa aggtgatact atgggtatta taaaatatta tactaattca
    13261 ggtgacttac ttggaagtgt agatatagta agtaataata gtatagataa cgtatcatta
    13321 aaaacaaaat taaaaatgaa atttgcaaat taaattcaaa aaatggtata aaatacacaa
    13381 aaaaatataa aaatagaaaa tatggaaaaa atattgttat ttcttcacct ctgtgatata
    13441 ctactagagg tgaaaaaata aaataaatca cacacagcat tgggatcctt aaaaggtgcc
    13501 tattacatgg ttttttaggg agaagatcaa gctggaggta taaaaccagg aggtaagata
    13561 tgtcagtaat atcaatgaaa caattattag aagcaggtgt tcactttggt caccaaacaa
    13621 gaagatggaa ccctaaaatg gctaagtata tattcacaga gagaaatgga atatatataa
    13681 tcgacttaca aaaaacagtt aaaaaagttg aagaagctta caagttcact aaagaggtag
    13741 ctgaaacagg aaagccaatc ttattcgtag gaactaaaaa acaagctcaa gatgcgataa
    13801 aagatgaagc tgaaagatgt ggaatgtact ttgttaatga aagatggtta ggtggaatgt
    13861 taacaaacca caaaactata aaaacaagaa taaataaatt aagagaatta gaaaaaatgg
    13921 aagaagaagg cgtatttaat gttcttccta aaaaagaagt tataaaatta agagctgaaa
    13981 aagaaaaact agaaaaatac ttaggtggaa taaaagacat gcctgaatta ccaggtgcaa
    14041 tgttcgtagt tgacccaaga aaagaaaaca tagctattca agaagctcat agattaggta
    14101 taccagtagt tggtatagtt gatactaact gtgacccaga acaattagac tttgctatac
    14161 caggaaatga tgacgctata agagccgtaa aattaataac tggtgctatg gcaacagctg
    14221 taattgaagg tagacaaggt gctgaggaag aagtagctga agaccaagaa taataatttg
    14281 acaattaaat ggtaagggtc gcccttacca tttataatat ggaatagaat aagtaggagg
    14341 aaacttaaaa aatggctaat ataactgctc aaatggtaaa agagttaaga gaaagtacag
    14401 gcgcaggaat gatggactgc aagaaagctt tacaagaagc tgaaggaaac atggaaaaag
    14461 cagtagattt attaagagaa aaaggattat caaaagctgc taaaaaagct ggtagagttg
    14521 ctgctgaagg tctagttgca atagaaatga atgatgataa tacagttgct tctatggtag
    14581 aagttaactc agagacagat ttcgttgcta aaaacgaaga cttcaaagtg ttcgttaaag
    14641 atgctgcttg tatggcatta gctactgata aagaagatat agcttcttta ttaggtgaaa
    14701 ctcataaaga gggaataact ttacaagaag ttttaaataa tagagttgca aaaataggtg
    14761 agaaattaga ctttagaaga tttgctaaag ttgtaactaa tggacaagtt gctggataca
    14821 ttcacggtgg tggaaaaata ggtgttcttg ttgaaatgga aacagaagct agagatgcta
    14881 aagttttaga attaggtaaa gatgtagcta tgcaagttgc tgctatgaat cctaagtatg
    14941 tttcaagaga tgaagtagat gctgaataca tagcacatga aacagaagta ttaactcaac
    15001 aagctttaaa tgaaggaaaa ccagctaata tagttgaaaa aatggttaaa ggaagattag
    15061 aaaaagaatt aaaagaggtt tgtttattag agcaaacttt cgttaaaaat ccagatataa
    15121 ctgttaaaca attagttgct gatgttgcta aggcagtagg ttctgatata aaagttgtta
    15181 aagttgtgag attcgaagtt ggtgaaggta tacaaaagag agaagaaaac tttgcagaag
    15241 aagttgctaa gcaacttaag taataaccaa agagagaaca cgcaggtgtt ctctttttta
    15301 aaaaatagaa aaaattggat atgaaggatt gaaataggag gtttctgatg gataaaccaa
    15361 tgtacaaaag ggtattgtta aaattaagtg gagaagcatt agcaggagaa agaggttttg
    15421 gtataaataa tgatgtagtt aatgatattg ctattgctat aaaaaaaata caagaaatag
    15481 gtgttgaagt agctgtagta gtaggtggag gaaacttctg gagaggcaga accagtgaag
    15541 gaatggacag aactactgct gactatatag gaatgttagc tacagttatg aatgcaatgg
    15601 cgctacagga tgcactagaa aatatagatg ttgctacaag agttcaaact gctatagaca
    15661 tgagacaaat tgcagaacca tatataagaa gaagagcagt tagacactta gaaaaggaaa
    15721 gagttgttat atttggtgca ggaacaggaa acccttactt tacaacagat acaactgctg
    15781 ctttacgtgc tgctgagatg gaagcagaag ttatattgtt agctaaaaat gttgatgcag
    15841 tatatgacaa ggatccaaaa gtacatgcag atgctaagaa atttacagaa ttaagttaca
    15901 tggaagttat acagaaagaa ttaaaagtaa tggattctac agctacatct ttatgtatgg
    15961 ataataaaat tccaataaag gtatttgagc ttacaacaga gaatataata agagcagtaa
    16021 aaggcgaaaa tataggaact actgtaaaat aatgaagaat gaaggaggag taaaattatg
    16081 aaactggaaa tacataagca attagaagag aaaatgaatg ggacaattga tgctttaaag
    16141 tttgaatttg gaactataag agcaggaaga gctaatgcac aaatgctaga caaaataaga
    16201 gttgattatt atggaactcc aactccaata aatcaaatag gtgctatatc tgtaccagaa
    16261 ccaagaatat taatgatatc tccttgggat aaatctgcta tgcatgaaat agaaaaagca
    16321 atagctaact cagacttagg attaaatcca tcaaatgatg gtgaggttat aagactttct
    16381 gtacctgctt taacagaaga aagaagaaaa gaattagcta aaaaagctag taaagctgca
    16441 gaagaattta aagttagaat aagaaatgaa agaagagacg ccaatgagaa gattaaaaag
    16501 atggaaaaag gcggagaatt aactgaagat gaattaaaaa aagcacaaga tgaagttcaa
    16561 aaaatgactg ataaatttat aaaagaaata gatacattat tatctaaaaa agaaaaggat
    16621 ataatggagg tataatattg aaaaaatatt ataacctttt aggtcttcat gtagatgagg
    16681 taaaacaata ttttgatgag caaaatataa attataccgt aaaatctatt gaaggtaaaa
    16741 aggataaaga aaaacttgta gttcctagag ttataaaaat atctgagata ggtgatagtg
    16801 tagaactgat aatcacatac ttttctgact ccttagttta aggagtcaga acaatattgg
    16861 aggcaaaata tgaataacaa cattatatat gatatagact taaataacat acctactcat
    16921 attgctatta taatggatgg aaatggaaga tgggcaaaag ctagatttct tccaagaact
    16981 gcaggacata aggctggagt agaaactata agagatatag taaaagaatg ctcaaaatta
    17041 ggtgttaagc acttaacatt atatgcattt tcaacagaaa actggaaaag accgaaactt
    17101 gaagttgaca cacttatgaa cttattatct acatacttaa gaaatgaaat agctgaactt
    17161 caccaaaaca atgtaaaagt aactgctata ggagatataa gtgctttacc aaaaacttgt
    17221 ataagagaat taaactcagc taaggaactg acaaaaaaca atacaggagt taacttaaat
    17281 ttagcattaa actacggaag tagagcagat ataaaaaatg ctcttataga tatagtaaaa
    17341 aattgtgaaa gtggtaaaat agatataaac aatattgatg aagatattat aaaaaactat
    17401 ttaagcacaa agtctatacc agatccagac ttggtaataa gaacaagtgg tgaacaaaga
    17461 ttgagtaact ttttactttg ggaagttgct tattctgagt tttactttac tgatatacat
    17521 tggcctgatt ttaaaaaaga agaactacag aaggctatat acgtatatca aagaagggat
    17581 agaagatttg gaggacttaa gtaaggagat tattttatgc ttacaagaat tattgcttca
    17641 ctagctctgg ttccattatt tctatttgtt gtatatggag gtattcctct atatatagct
    17701 gagacagcaa tagtttatat tgcattgcat gagttttata aggcatttaa aataaaagat
    17761 gtacatccaa tattcattat aggatattta ttttctattt atttagctgt aaaaaatatt
    17821 tttaatttac cattagaata tacttatgca gtaattttta ttttattttt agcaagtata
    17881 atttatatgt tgatgggcaa aaacaatgtt atagatgttt cgattacgtt tttaggagtt
    17941 ttttatatag gagtattttt agattttata attataacta taaatggatt tgaaaaaggt
    18001 agtatatatg tatggcttat atttgtgata tcttttatga cagatatatt tgcatatttt
    18061 agtggttatt tacttggaaa acataagcta attcctaaag ttagtcctaa aaagactata
    18121 gaaggagcta ttggaggaat aataggaagt acactttgtt gtattttatt tggatattta
    18181 tttggcattg atttgcttca attagcgata ataggaagta taggaagtgt aatagctcaa
    18241 ttaggagatt tatttgcatc ttctataaag agatatgtag ggataaagga ttatggaaag
    18301 ataatacctg gtcatggggg tatattagat agatttgata gtgttatatt agttgcacct
    18361 tttgtatata gtgcgattaa attttttatt agataaagat tggttggatt taaaagagca
    18421 atctataatt gctagtaatg aaaatagcta tgtaactttt aaatgcagag atattctgca
    18481 gttattagtt acatggctat tttcattgtt caaaaattta gaagaccttg gggaattgta
    18541 taagaatagg tattacttta taatagtata ctatatttgt agtgagtata ttttttata
//
LOCUS       DAENCV010000072        18533 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAENCV010000072 DAENCV010000000
VERSION     DAENCV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.073"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            68..141
                     /locus_tag="KQD82_002477"
     tRNA            68..141
                     /locus_tag="KQD82_002477"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            178..1140
                     /locus_tag="KQD82_002478"
     CDS             178..1140
                     /locus_tag="KQD82_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4380943.1"
                     /translation="MFPFRTQKLSKVNGITKDSVTNNRVYGADRHETNANVIKKFYTD
                     DELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKI
                     GGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKDYN
                     LNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNKQG
                     HGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRS
                     NDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            1382..3295
                     /locus_tag="KQD82_002479"
     CDS             1382..3295
                     /locus_tag="KQD82_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380944.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFGDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            3288..5507
                     /locus_tag="KQD82_002480"
     CDS             3288..5507
                     /locus_tag="KQD82_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG4380945.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5616..6446
                     /locus_tag="KQD82_002481"
     CDS             5616..6446
                     /locus_tag="KQD82_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG4380946.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            6436..9903
                     /gene="addB"
                     /locus_tag="KQD82_002482"
     CDS             6436..9903
                     /gene="addB"
                     /locus_tag="KQD82_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG4380947.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISTLEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9903..13730
                     /gene="addA"
                     /locus_tag="KQD82_002483"
     CDS             9903..13730
                     /gene="addA"
                     /locus_tag="KQD82_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG4380948.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13776..14987
                     /locus_tag="KQD82_002484"
     CDS             13776..14987
                     /locus_tag="KQD82_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG4380949.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14974..18501
                     /locus_tag="KQD82_002485"
     CDS             14974..18501
                     /locus_tag="KQD82_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4380950.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
BASE COUNT         8050 a         1739 c         3295 g         5449 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagcaag gtaaatggaa taactaaaga tagtgttact
      241 aataatagag tatacggagc agatagacac gaaacaaatg caaacgtaat aaaaaaattc
      301 tatacagatg atgagttaga ggctgtttta gtagctaaat cagatgtact tgttgatgct
      361 ttagcagcag gtccattggc tgcgaactta aaatctccaa tacttataac accaaagacg
      421 tatgtatctg cataccataa agataattta gaagctaaat cagctaataa ggtatacaaa
      481 ataggaggag gattgacttc taaggtaatg agctctatag catcatcatt atctaaacac
      541 aatacgactc caacagaacc aggaaatagt gggggcaaga cagttatgat tgacccaggg
      601 catggtggtt cagcacctgg aaattcatct ggaggaatga ttgaaaaaga ttacaattta
      661 aatacttcac ttgcaacaac tgaatattta cgttcaaagg gattcaatgt aataatgaca
      721 agagacacag ataagacttt atctcttgga aatagaactg ctctatctaa ttcattgaaa
      781 ccagatttat ttacaagtat acattataat ggctcaacta ataaacaagg tcatggtgta
      841 gaagtatttt ataagcttaa agataaaaat ggagggacta ctaaaactgt agctaccaat
      901 atattaaata gaattttaga gaaatttaaa cttacaaata gaggtataaa aacaagagta
      961 cttcctagtg attctacaaa agattattta tacgttttaa gaagtaatga tatgccagct
     1021 gtacttgtag aatgtgcatt tttggataat gaaaatgata tgagtttaat aaactcatct
     1081 gcaaaagtaa aagaaatggg tacacaaata ggtaaaggaa tagaagattc attaaaataa
     1141 atggaaataa aaatatttaa aaacttaatg taaatttata gttgtaaatt tatactaaag
     1201 tcaaaaaatg aaatataagt gtatcaatag taagactgta tgtacatttg cacatgtgaa
     1261 tgcaagtata tatacagtct ttttttgtag ttaaatgcaa taatttgatt gtgttttgta
     1321 aaatacacaa tatgatataa ttagataata tacacttaat ttaattgagg aaggtgatta
     1381 tatggaatat ttatttgaaa aaagtagttt aaaagaatat ggaaatgaag ctttagataa
     1441 atatagagaa ttattagata aaactggtaa taattcaagg tgtattcttt tgttagtacc
     1501 aaacaataac acaaagatac gatatgaaag ggctctaaga ttaaattata gtgaagaact
     1561 gaaaataaca acatatataa gttttgtgaa gaaagaatta gttaaatact ggccactaat
     1621 aatagaaaaa tgtgatgaaa taagtacaaa ggttgtatct ccaacattta tatcaaatag
     1681 tttaagtgaa tatttaatag tgcaaaaagt taaggaaaaa aggatacaag aaggatattt
     1741 tggagatatt acaggaacca ataaatccat agcaaatagt ataatgacta acataaataa
     1801 gtctgcattt aatttaattg attttgattt tattggagaa aaaatatatt catcaaagaa
     1861 aaataaagat agtatatata gattttcata cactcaaatg gatgaaatta tatcatatta
     1921 tataaattcc ttactatcaa attcaatttt agataatgca ttaagcatat atttatacaa
     1981 tcaataccta cttaatgatg atttttattt aaaaaactta tttacagaag tgagatatat
     2041 aattgtggat agccttgaaa gctcaagtaa tgcagaagtt gattttataa cagaagcatt
     2101 ggataataca ttacaaagtt atatatattt tgattatagt agggattatt ctgtatttaa
     2161 taatgtggat atagactata taaatgagaa aataatatca aaaattaagc ctaaagaaga
     2221 aaatattaaa aagaaggaga ttagaataga agatttatac ttattacctg caaatataga
     2281 attaaatcaa tcaagtcaat tatataatga aatgttagat ttaataagtg aaaaagttat
     2341 atctcttata gaagaagatg tatctccaag agatatagca attatatctc ctataaacaa
     2401 tagtatattg gagcatcaaa ttagagattc tttgatagaa aaaaatatag atgtttttag
     2461 tactaagaag gataaaaaag ctgtggatta tccatatggg aatgcattgg tagtggctac
     2521 atgtatattc tatggatatt tagattttat aaaagatgat gaatacttaa gctttttaga
     2581 aactctttta gaaataaata ggataaaagc atttaagatt tttaatgaaa caagacattt
     2641 aagtattgat aatatagatg aacatagtca gtatcgtgat attttacagt atattgaaga
     2701 aaaaaagaag agtgatataa aaatacatga atttttaact caattttaca tagataaaat
     2761 gttaaattta aaagaaggta aaaagaatgt tgggttatgt aaaaagataa tagctgaaag
     2821 tgagtctttt agtgaaagtt taagtctact tgggatgaaa aaagaaaaga tatttataga
     2881 agctttaaaa agtacaataa acgattatta ctctgttgtc gatatagaag agttgaaatc
     2941 caaagacaaa atagtaataa ctactcctta ttcatatata tcatcaaata tagatagaag
     3001 tatacaaata tgggtagata taggaagtaa tgcttggaat atgaaaatag aaaaagatat
     3061 aagtaattta attgtactta gaaaatcttt tgaagaaaaa aagatttata caaatgaaat
     3121 ggaagaatat tataaaaaat attatctata caatacagta tataatttgt tattaaatgc
     3181 aaagaaaatc tatgcattta aaagtgaata tgcgattaat ggttatatcc aagagagtat
     3241 attgtatagt atacttttaa aaatctcaca taaaggagat aaaaattatg attaatataa
     3301 attatagaga agaccagata ccaataataa attacgataa tggtactatg gcagtaccag
     3361 cagtaccagg tgctggaaaa acatttataa taacaaattt agtagctaaa ctattacttg
     3421 aacagaaaca taagggtggt aaaatattaa ttcttacata tatgaatagt gctgtaaata
     3481 attttaaagg tagaataaga aagattcttg aagagaataa aatagatgat actaattcgt
     3541 atgaagtaat gactatacac agtttggctg taaaaataat aaaggaaaag ccagaagttg
     3601 taatgttaag tgaggatttt aatatagctg atgatttgca aaaaagtatt atattaaatg
     3661 aatgtataag tagatttaga tttgaaggtg gagaaagagc ttttagatgg tttttaaagg
     3721 agcaaaaaga tgaacgctgg aaagaaataa ctcttgaagc ctgggaaaga ggtttttttg
     3781 aatttgcagg aaatgcaata agtgaactta agtataaggg aatttctcca gataaacttg
     3841 aagaaatatt gtcaagagga cacaaaggaa tgcttaaaat tattttacca atatataaat
     3901 tgtatgatag aaaattaaag caaaatggat tgctagatta tgatgatata ttaattttag
     3961 cagaaaaaac tttaagctta gacgaagggc ttagaaagaa gtttcaaact agatacaaat
     4021 atatatttga agatgagtgt caggactcaa atgaaattca aggtaatata ataaaaatta
     4081 tatctagcga aaataaaaac ctagttagag ttggagatat aaatcagagt ataacaggaa
     4141 cattttcatc atcagaccct aaatttttta aagaatttat aaatagtgca gatttctgtt
     4201 ataggatgga tatgtcaaat cgaagttcaa aagatatact tgatttagca aacactcttg
     4261 tcaagtatgt tacaaaggaa tttagacaga aagaatgtag acatgctctt gaagatatgc
     4321 aaataaaaac tgtgccaaat ggaatgggat ataaagaaaa tcctaatcca gaacattata
     4381 atataaatgt aaagtggtat gagagttgga aaattgaaat agagcaaact gctagatacg
     4441 ttaagggtat aaaaaataaa tatccagata aaagtatagg tatattggtt ccatttaatg
     4501 aacaagtgac acaagttgct aaagaactta gagagaataa tctaaccttt gaagaattag
     4561 gaccaaattc tttgaataaa aggaaggtta taaacaatat agcatatatt attgatttta
     4621 tgttacattg tgatgatgta gaaaagttaa tagttgtact taataaagtt tttatagata
     4681 ctgataatga agaagaaata aagtattttt tagaagaatt aaaaaactat tctaccgaag
     4741 aaattattta taattttgaa tttagtaagt tagataatga tttaatagaa tttaaagaag
     4801 aaagtgatat atacttaagt tttaaaagag gaatagatat tataaaacag atattagaat
     4861 atccaataat aaggttagac ctcttagttt tgtttattgg gaaaaaactt aatttagaaa
     4921 aagaagataa agctgtactg gattatatat ctttttatat aaagtatcta gtatcagaaa
     4981 atataaatat ggatttagaa gatgtatata atttattgtt tgatgtaaag aacagggtgt
     5041 ttaatcatat aatagatgtg gtatatgaga taaatggata tgaaccagag cccggtagta
     5101 taacactatg taactatcat aaatcaaagg ggatggaatg ggattgtgtg tttttactag
     5161 gtcttgttga atataatttc cctgacaata ttaatcaaaa gtttcagagt gataaatggt
     5221 atctaaaaga aaaatataaa aaccctatgg caattattaa atcagaagtt gaagctatat
     5281 taaaaggaag tatttcaaca gattatgctc ataaaacgaa gattgattct ataaatgaaa
     5341 agattagact tttatatgta gggattacta gagcaaagga aatgttagta ctttcaggaa
     5401 gtgcttatag agatgaaagt gatataggga ataagagaaa agagcaaaaa ccatgtatat
     5461 atttaagcag acttaatcaa catattattg aaaaaaggtc aaattaatat ataataagtt
     5521 attaaaagat tgcttgaaat atatctaaaa ggggataaaa caatatgtac agtaagtaac
     5581 tactaaaaaa taaagttaag agagaagtga tatgagtgaa cgataaatta aaatattttt
     5641 cttatagtca aaattcaatt aacacatata aatcatgtcc attgaaattt aaatataaat
     5701 atattgatag aataaattgg aaaaatgatg atgttggtag tagagagtat tatgaaactt
     5761 taaaaacagg aagagatttt catttgattt gtgaaagata ttttagcaat atacctcttg
     5821 gaatctattt taatgaggat gataaaaata gtaaaaagtt tttaaagtgg atggaaaata
     5881 taaaaaaagt tgttcctatt gaaaaaggaa aaacgtattt gccagaatat gaagtaagga
     5941 tgacattaaa tggggatata atacaggcaa agtatgattt agttgtaatt ggaaataaca
     6001 atatagaaat atgggattgg aaaaccgagt ctaaaaagat tgactataaa cacgtagaaa
     6061 atagaataca aactattgtt tatatgtttt tagcaaaaga agtaatacct aaagttttaa
     6121 aaatggacat aaatgttaaa gatataaata tgagatatta tcaacctgaa tttgatgact
     6181 taccaattac cattttatat gatgaagaaa aacatgaagc aaatagaaat aaaattcaaa
     6241 attatataag tatgataaaa aacactaatt atgaagaaca tacatacgaa gacaatctat
     6301 acaatgatat agaaaaggta tataataaag aagaacgtat gtgctataga aataaaaagc
     6361 attgcaagta ttgtgaattt aataagttat gtaatggcaa agaaatagat tatagtattt
     6421 tggaggcgga aatttatggg acttaggttt gtattaggta gaagtggaag tggaaaatcc
     6481 acctatatac tagatgagat taaaaaagaa gctcaaaaaa atgaaacgac atcaattata
     6541 cttttagttc cagaacagta cacatttgag gctgaaaata gagtaagtaa gctattctta
     6601 ggaaaagaaa aagataagta tctaagagtt agggtactta gctttaagac actaagtaat
     6661 atagtttttt ctcaagtagg aggattgaca gatgtaaata taaattctag tggaaaagca
     6721 atgatggtat atagagctat tgaagatgta agtgaagaat taaatgtgtt ttcaaaatca
     6781 aaatcgcagt ctggttttgt aagttcaatt acagatatga tatcagaaat gaaacaatac
     6841 aatataagcc ctgaaatgtt agaaaatata tctggagaat tggataatga aactttaagt
     6901 ttaaaattaa aagatataag taaaatatat aattcttttg aagggaaatt gcatgaaaat
     6961 tatgtagatg ctcaagatat gttaacttca ttggcaagta aaattgaatt aagcagttat
     7021 ttagatggag cttgtgttta tatagatgaa tttacaggtt ttacaccaaa tcagtacaat
     7081 gtcataaaga gtatattaaa taagtctaaa agtgttaata tctctttgac tgtagatgat
     7141 atcaactata ttgggtacag taaatctgat atgttttcta gaacgaaatt tacttattca
     7201 aaattgactc aattgtgcaa cgaagaaggc ataaaaatat tgccacaagt aaatttaaac
     7261 acaggtgtta taaaaagatt tgaaaaagtt aaggaattac agcacttaga aagattttat
     7321 aatgcttatc catataaaat atattcaaat ccaactgaaa atataaagat aaaagagttt
     7381 aacaatttat attcagaggt ggaagagata gccagagaaa ttgtacattt agttagagat
     7441 aagaatgtca gatatagaga tataaccatt gctacaagag atttaaatag atatgatttt
     7501 ttagtacact ctatatttaa tgaatataat ataccaaact ttatagataa gaagagagag
     7561 gcaaaaagta atccaatagt tattttaatt atttcaactt tagaaatgaa gaatagacga
     7621 tatgggtatg aaactatgtt taggtattta aaaagtggac ttataggaat tgataatgat
     7681 gatataaatt tactagaaaa ttatgtgttg gctaatggga ttaaaggtaa aaaatggttt
     7741 gatgaaaaat gggattatag aataactcaa agtctttcag gtcaagaaag tgaatttgaa
     7801 ctggaattaa aagagaaaat aaatgaaata aaaaatagag tattagagcc tatagtaatt
     7861 ttacaagaaa aattaagagg aaaaaataga gtaaaagaaa tctgtagata tatatatgaa
     7921 tttttattag atataaatat gccagaaact atagaaagtt taatagtaaa ttttaaggat
     7981 aaaggggaac ttgatgttgc aaatcaatac tcacaagttt gggacatagt agtggacata
     8041 ttagaccaaa tggtggaact tatgggtgat gaaataattt ctttagaaaa atttataaaa
     8101 ttaataacct taggttttga tgaatatgaa ttaggactgg ttcctcctag tatagaccag
     8161 gtacttgtaa gtagtgtgga tagaatgaaa aatccagaca cgaaatattt atacctagtt
     8221 ggaactactg atggtgtgtt tccgctgatt acaaaggata gtggaatatt gagtgataat
     8281 gatagggaaa gtcttggaaa caaaggaatc gaagttgata ttgatagtaa gactaggagt
     8341 tttgaagaac agtttcttgt atataaagct cttacttcga catctaagaa tttaaccata
     8401 acatatccaa tatcagacca tgaaggtaaa acgcttagac catcaataat tatatcaaga
     8461 cttaagaaga tatttccaaa tatagaaaat aaaagttatt tagtggaaga aaataaaagc
     8521 actgataaag atatacttaa aaaaataacg gttaaatccc ctacatttaa tgaacttata
     8581 aacataataa aaaattatga cagtgatggt tacaatacag aagagattaa tagtatttgg
     8641 ttagatatat ataggtacta tttaaaagat gaagtctata gttctataac aaaaaaagtt
     8701 ataaaaggtc ttagttatac taatcaagtt cataagattg aagagaaaaa aataaggtca
     8761 ttgtataaaa gtaattctct tagcatatct aggcttgaaa aatatgctga atgtccattt
     8821 gcttatttta tacaatatgg tctaaaagca aaaaagcgta aagagtattc atttacacca
     8881 ccagatttag gaacttttat tcataatatt ttagataggt tttcaaaaga acttttacaa
     8941 gataatttaa cttggagaga tattgatgaa aaatatatag aattaaagat aggtataata
     9001 gttgatgaaa taattttaaa gataccagga tatatattaa atagttctga gaggtataaa
     9061 taccttgcat atagattaaa gaatatgttg acaactgcta taactataat tagtcagcaa
     9121 ataaaacaag gttcatttga accaatagat tatgaagtta aatttggcaa caatggaaaa
     9181 tatccaccta taaaaatggt gttagaaaat ggacaagaag taagtttaat tggacaaata
     9241 gatagagttg atgaatttga agaaggagaa aataaatata taagaataat agattataag
     9301 tcaggcaata agtccataag tctaacagaa atatattatg gtctacaatt acaattactt
     9361 gtgtatttag atgcaatcct tgaaagtgca aaggatgaag atataaatat aaatccagct
     9421 gcaatactat attgtagaat aaataatcca atagcaaaat ttaatgaaga taaagatgat
     9481 gaagaaatac aagaagcaat attaaaagaa cttagaatga aggggcttgt agtaaaagac
     9541 tctcacatag ttaaagagat ggataaatca ttaatagatg gagaaaggaa aaactcttta
     9601 gtaataccag taggtcttac taaggatgga aatgtaggta aaagtacttc agcaataagc
     9661 tatgaggatt tcaaactgct tagaaaatat gtaaggcatg ctataaagga tttatgtgaa
     9721 gaaatgttaa gtggagaaat acgtatagct ccatataaac ataaagatgg aacatcatgt
     9781 gacttttgtg attactctgc tatatgtcag tttgattcaa ctataaaaga taacaagtat
     9841 aaaaatttaa ataataagag taatgaagag ataataaaaa tgatgaaagg agatgttaat
     9901 tagtgagttc tcctaaatgg acaaaagagc aattagaagt tatagaaagt agagaatgca
     9961 atttattggt tgctgctgct gctggttcag gaaaaacagc cgtgctagtt gagcgtataa
    10021 ttcaaatgat aacaagtaga gaaaatccaa ttgatataga taagttatta gttgttacat
    10081 ttactaatgc tgctgcgtca gaaatgagag agaggatagg ggatgctata ggaaaagcct
    10141 tagatgaaaa cccagaaaat aaacatttac aaaaccaact tgttttatta aataaatcta
    10201 gtattacaac aatacactca ttctgtttgg atgttataaa atcaaacttt catagaataa
    10261 acttagaccc taatttcaga ataggagacc aaactgaatg tgctattttg aaacaagaag
    10321 caatagagga agtatttgaa gatttatatg aagaaagaga tgaggggttt ttaaatttag
    10381 ttgaaagtta tgcagaaaga ggaggagata aagaagtaca agatattatc ttgggaatat
    10441 attcatttgc aatggcttct cctgaaccta aaaaatggtt gattgactca gcagaacgat
    10501 ttaatattga tgaaaatttt gatttttcac agtctatttg ggctagagct atattagaca
    10561 ctgtaaaaat agagattaat ggtctttgtc taaatatgga aagggcatta aaagaagtgg
    10621 aatcaataga agaattagaa acttttgcag aaaagttatc tgttgaatat aaaaaaattg
    10681 ctgacatttc acaagcatgt aataaatctt gggatgaggc atataaaaag atggcaagta
    10741 tgtcctttga aaattatgta aaaggagtaa aaagaatttc aaaagatgct ccttcgtata
    10801 taaaagaatc aaaagaaaag gcaaaaacta taagagataa gactaagaag tctttagaaa
    10861 gtatagtaag tgctacattc aataaagata atgattctat tagagaagaa ataaaatatc
    10921 tttataacat agttaaacca atatctagtg ttgtactgag atttgaagag gaatatagca
    10981 ataagaaaag agaaaaagga ataatagatt ttaacgatat agagcatttt gcattaaata
    11041 tacttacaga tgtagatgaa aaaggtaata ttgtaccttc tgatatagca gttggatata
    11101 gaaacaagtt ttatgaaata tttatagatg aatatcagga tagtaatcta gtgcaggaag
    11161 tactgcttaa agctgttgca aatacagaaa ctccaaatag atttatggtt ggtgatgtaa
    11221 aacagagtat atatagattt agacaagcaa agccagaatt atttttgcaa aaatataata
    11281 actataatga taaaaaggga agttcccata gaaaaataat gctttacaaa aattttagaa
    11341 gtagagaaga agttgtagat gctgtaaatt atatatttga aaatataatg aatgagaata
    11401 taggagaaat tgaatataca gaaaaagaaa gactaaattt aggtgcaaat tttaatgtgg
    11461 atacagacga gaaatctatt attggtggag caacggagat acacctaata caaaaagata
    11521 ataaacttga tgatgatatt ataaatgata aagacgatag aattaataac aaagaagatg
    11581 aaatagagga agaagaaaaa ttagataata tacaacttga ggctagaatg gttggaaaca
    11641 tcatcaaaga tttaatgaaa gtcaatgaag atggaaaaat tcaaaaggtc tatgataagg
    11701 gaatagatgg atatagacct gtggaattta gagatatagt tattctttta agagctacat
    11761 ctgcatgggc tccagtgttt gcagatgaat tgatgaatat ggatatacca acatatgctg
    11821 atgttggagt ggggtatttt gatacgattg aaataaaaac aatattgtca ttacttcaaa
    11881 taatagataa tccaatgcaa gatatacctc ttatatctgt attaaaatca cctatatttg
    11941 gatttacacc agaggatttg attgatatta gagttcagag taaagataaa attttttatg
    12001 aggttttaaa aagtacagca gagtatgatg gatttacaga ttctcaaaat gaaaatgagt
    12061 ctgagtttat accaagtgaa gagtgcataa acaaaagtaa agatttttta attaagctaa
    12121 aggaatttaa agaaaagtca atgtacatga gtacagatga atttatatgg tacttgtaca
    12181 caaggactgg atactatgcc tatgttggag cattgccagg tggaagtcaa agacaagcta
    12241 atttaaaagt attgtttgag agagcaaaac aatttgaaga aaccagtctt aaaggaatat
    12301 tcaactttgt aaattttata gagaaattaa aaaaatctag ttctgatatg ggaagtgcaa
    12361 aaacacttgg tgagaatgca aatgttgtta gaataatgag tattcataaa agtaaaggtc
    12421 tagaatttcc agtagtcata tgctctgcaa tgggtaaaaa tttcaatact caagatttta
    12481 aaaagagtat tttatatcat cataatttag ggtatggacc tcaatttgta gactatgaga
    12541 gaaggatttc ttttccaagt atagccaaag aagcattaaa aagcaaaata aatatagaaa
    12601 atttatcaga agagatgaga gttttatatg ttgcatttac aagagctaaa gaaaaattga
    12661 ttataactgg ttctactaga aatatacaag atagtataaa gagatggtca aatggaattg
    12721 aaagtttaga tacaatatca caatacgaga tattaaaagg taaaaatttt ttagattgga
    12781 taatgccttg tgtattaaga catagggatt tatctaatct actagaagaa gtaggattag
    12841 acgcagtttt taatgttgaa cataattcaa aatggtatgg aaagttgtgg aataagaatg
    12901 atattttagt ggaaaagaag agtgatgaag aaaaagaaag tattgaagaa attcttgaaa
    12961 aaatagatgt agataatcct gacagtgatt attacagtga aatagaagaa aaattaaact
    13021 atatatatcc atatgaattt agtacaagaa aacctgctac tatatctgtt acagaaataa
    13081 aaaagataca aaataattat gaagaagagt taataaatac tatatttgaa caaaaagtaa
    13141 tattaaaaaa gcctctattt attcaaaatg aagaagaaag agaaaaaata agtggaacag
    13201 aaagaggtac aatagtccat ttagttatgg aggttttaga cttaaaaaat gtaagtagtg
    13261 taaatgatat aaaatcacaa ataagaggtt ttgtttcaaa aggtataata acagaaaaac
    13321 aagctagtat agtaaatcca tataaaatat ataaattttt tgcctctaac ataggaaaaa
    13381 gaatgttgaa tgctgaaatt ataaatagag aaaaatcaat ttatgcacaa gtaaatatga
    13441 aagacatata tatttatgaa aagcttataa acaatgacga taaaaaactt tatgataatg
    13501 aaagtgtcat gttaagaggt atagttgatg cttattttga agaagataat caaatagttt
    13561 tggtggatta taaaacagat tttgtaaatg aggaaaatat aaatcaaata atagagaaat
    13621 ataaaaaaca gttggattta tatgcagata ttattgaaac tttaactggt aagtcagtaa
    13681 aagagaaatg tatatattta tttggagttg atgaagctgt ttgttattag tttttatata
    13741 taaattaaac ctaaaagata gaggaagggg aaaaaatgag atttattcat acatcagatt
    13801 ggcatctagg gaagtcccta gaaggccatt ctagaattga ggaacaagca aagttttgtg
    13861 aggaatttat aaaaatagta gaatcaaatg aaatagatat ggttataatt gctggagatg
    13921 tatatgatac ttctaatcca ccagcacaag cagaaaaact attttatcag actgtatcaa
    13981 gacttgccaa taatggtcag aggtgtgtat taataatttc aggaaatcat gataatccag
    14041 agagactctc tgcaataaca cctctagcac atgaacaggg gatacttata tatggttatc
    14101 cattaagtgc tacgatagaa gctaagtata aagggtttga aataacatat gcaactcagg
    14161 gatgtacaaa actgaatata aatggagaaa atattgtggt tgctacatta ccttatccaa
    14221 gtgaaaaaag attaaatgaa gttttttcaa gtgaagatga gtttgagaaa caaaaaaatt
    14281 attctgaaaa ggtaggagat atatttagaa gtttagaaga aaattttaga agtgatacta
    14341 taaatatagc cgtatcacat atatttgtaa taggtggaga gagtactgaa tcagaaagac
    14401 ctatacaatt aggtggaagt tttttagttg aaagaaaaga tttgccagaa aaggctcaat
    14461 atactgcact aggtcattta cataaacaac aaaaagcttc cgaacgttta aatgcgtact
    14521 attctggttc tccacttcaa tatagcaagg atgaaagagc gtataccaaa ggggcataca
    14581 tagtggatat aaaagctggt gaaaaaccaa taattgaaga tgtatatttt aataattaca
    14641 aacctataga agtatttaaa tgtaatggaa tagaagaagc actagatata tgtgagcaaa
    14701 atcaagatag agagatttgg tcatattttg aaataaatac tgatgagata atatcacaga
    14761 atgaaattaa aaaaatgaag gaactattaa aagacataat agaaataaaa ccaataataa
    14821 catcttgtta tgaacaagaa agtgtagata taaaagaaaa gtctatggca gagttgttta
    14881 gagagttcta ttcttttagt aaaggtgtag agccaaaagg agaacttatg gatttatttc
    14941 tagatataat aagtgaagaa ggtgaatcag cagatgagac caattagatt agaacttaca
    15001 gggcttaata gctatattga taaacaagtt atagactttg aaaaattaat tgaaagaggt
    15061 ttgtttggga tatttggaac tactggaagt ggaaaatcta caatattaga tgcaataact
    15121 attgctatgt atggaaatat atccagaaat acaaaagaat atataaacag tgtgtgcgat
    15181 aaggcaatta tatcatatga gtttgagata ggaagcaaaa acactagaag aagatacatt
    15241 gtagatagaa ccattgctag aagcaaaaca ggaacaaaga cctctaatgc tagattaata
    15301 gaagtattga atgacaatac ccaaaatgtt ttagctgata aagttgtgga agtaaatgaa
    15361 aaagtagcac aggttgtagg attaacggca aatgatttta ctaggtcagt agttttacca
    15421 caagacaaat ttaatgagtt tctaagatta tcacgtgcag atagaagaga catgttagag
    15481 agaattttta atcttgagaa atatggaaga agtcttggag aaaaagtgaa aaagagaaaa
    15541 aatatacagt tacaaaattt aaaagatttg aaatcaaaat taagtcaata tgatggaatt
    15601 acagaggaag tatataacaa tataaaccaa gaattaatag agttaaaaaa tttggaaaaa
    15661 gataagaata atgctttaga cctagctcaa aaatcttatg aagaaagtaa aacagtatat
    15721 gaagaacaat taaagttaga aaaaaatgaa cttagaaaaa atgagcttga tttaaaaaat
    15781 agtgagataa aagaaaagat tgctcttgta gaaaatgata gtaacgctaa aaaggtagac
    15841 ccatacataa gttcagttca aaatctagaa aaaaagatag atgaagatag cttcgcagta
    15901 agtaatttag aaaagaaatt agcaatatta aatcaggagc ttgaagttac aaaaaataga
    15961 tatgaaaaaa taaacaaaat taagaatgaa gaagtcccta aattgagtga agaaaaaata
    16021 agattgcaac aagcaataaa attagaagaa gagttagttt tattagatag agagttaaaa
    16081 gagctaaaag aaagtggaat taatttaaat aagactaaag ttgaattaga aaaagtaaag
    16141 caggtgtcag agtccagaaa agatgcagtt actaaatcta taaaagaagt tgaaggaaag
    16201 attgataaag taaatataag tgcagaatta aaacaaaaaa tatttctagc atatgaatac
    16261 gagaaagatt acaacaacgt attggaagaa aaaaatcaaa agctaaataa actagaagaa
    16321 atactcaaag agacagaaaa tataaattta aaagttagat atattgataa agataagaat
    16381 gatgtaaatc gtaatttaga aaatttatca cttcacttgg atgttcttct taaaaaatgt
    16441 cctggtaaga gtgctgattt acttttaaaa tctgaatatg ttactgaatt aagaaataaa
    16501 gcaaataata ctaaagaaaa tgaaattaag aaaagcagta tacaagatga attaaagata
    16561 atacttgaaa gtaagtttaa tactgaaagg gaagtaaatt tactaaatga aaaacttgaa
    16621 aataatagaa agaacagaga tgacctagaa aaagaattag aagaactaaa gtatttaaat
    16681 ttagcctctg aacttagacg tgaattaaaa gaaaatatgc cttgtccagt ttgtgggtca
    16741 aaacatcatg aaaatcataa tataactaaa tatgatgaaa atatatcttt tgtcaaagaa
    16801 aaactggaga aattagaaaa agaaaaaata agtataagaa acaatataga ggaattaaat
    16861 gctaaagtaa gtgggtattt atctatagaa aaaatgaaga ctaaagaact tgaagatgta
    16921 aaaggaaaac taggggggat tccatctagt caattattaa aaaaacttga tgaagaacaa
    16981 agaaaacttg cattgttaaa atctaatatt caggagtggg agaaagaaaa agagagtact
    17041 gaaaataaag taacattagc gaaagaagaa aaaaataaaa ttgaaaaaga agaattaaaa
    17101 ataagagaga gtctgaataa ttataagaaa ttaacaagag atttaaacat agaaatagaa
    17161 ggtttagaaa ataaatgtaa aaaacttaaa caggaatacc taggattaaa gacgattact
    17221 aaagtctcta atctattgtc aaaagtaaaa gaaataaggg agaatgaaaa agaattagaa
    17281 ttactaagta ctgggcattc aaatttatta aaaaatagag actcactaga tattaaaata
    17341 agagaaaatg agagtaaact acatgaaata gagatagaat taataaaggc tagagaatta
    17401 tatgtagaaa agaaattgag tagggataat aagtataaag aagttattag cataactaaa
    17461 ggtgatttag ctaaaaatct tttacataat gttgaagaaa atatatgtaa gatattagaa
    17521 caagaagaat caagcaagaa aaaattggaa gagcaaagat tagaatatga aaaaaacatt
    17581 gctgaaaagc acaatataga tggtagactt aaaactgcta aggaacaata taaagaccaa
    17641 aaatcaacac taaataaact attagcagaa aataaatttg aaagtatata tgcagtaaaa
    17701 agagctttat tagatactga tgcagtaaaa aaattgattg aagaaatact agagcatgaa
    17761 gaagaacaaa agttattatc atttaaaata aaatcatcaa aagaaaaatt aaatggaaga
    17821 agcataaaga aggagtactt tgaacaatta aaagatgaaa tttataattt gaaagtagaa
    17881 ataggaaata tatccaaaga tattggagct aatcaaaatc agttaataac attaaaagac
    17941 tctttagata agataaatga ttttaataaa caacttaagg tagttgaaca caatgtggac
    18001 ttattggaag aattagataa atgtgtacaa ggaaataaat ttgtagaata tgtttctaca
    18061 aaccaattaa aatatatagc gttggaagca tcaaaacgat tggatggaat aacaaaagga
    18121 agatatgcac ttgaaattga ctcaacacta aactttgtaa tgagagataa ctttaatgga
    18181 ggagaaagaa gaagcattga tactctatct ggtggagaga cattcttgac atctctatca
    18241 ttagcacttg ctctttcatc tcaaatacaa ttaaagggaa gtgcaccatt agaatttttc
    18301 tttttagatg aaggttttgg ttcattagat agtgaacttt tagaaattgt tatagaatcc
    18361 ttggaaagat tgcatagtaa taatctaagt gtgggtataa taagtcatgt cgaggaactt
    18421 aaaaatagag ttcctgttaa gctattagtt tcatctagtg aggctggaat aggttcaaaa
    18481 gtaaaaattg aatatagtta gaaaatatat gttaattgaa tatattatct aat
//
LOCUS       DAENCV010000073        18042 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAENCV010000073 DAENCV010000000
VERSION     DAENCV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.074"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(628..816)
                     /locus_tag="KQD82_002486"
     CDS             complement(628..816)
                     /locus_tag="KQD82_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380951.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            1070..1246
                     /locus_tag="KQD82_002487"
     CDS             1070..1246
                     /locus_tag="KQD82_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380952.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
     regulatory      complement(1463..1558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1767..2144
                     /locus_tag="KQD82_002488"
     CDS             1767..2144
                     /locus_tag="KQD82_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380953.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYRWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            2254..2637
                     /locus_tag="KQD82_002489"
     CDS             2254..2637
                     /locus_tag="KQD82_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4380954.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            2822..3406
                     /locus_tag="KQD82_002490"
                     /pseudo
     CDS             2822..3406
                     /locus_tag="KQD82_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3432..3845
                     /locus_tag="KQD82_002491"
     CDS             3432..3845
                     /locus_tag="KQD82_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4380955.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            3849..4592
                     /locus_tag="KQD82_002492"
     CDS             3849..4592
                     /locus_tag="KQD82_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG4380956.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            4592..5062
                     /locus_tag="KQD82_002493"
     CDS             4592..5062
                     /locus_tag="KQD82_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380957.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            5079..5837
                     /locus_tag="KQD82_002494"
     CDS             5079..5837
                     /locus_tag="KQD82_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4380958.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            5821..6663
                     /locus_tag="KQD82_002495"
     CDS             5821..6663
                     /locus_tag="KQD82_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG4380959.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(6823..7728)
                     /locus_tag="KQD82_002496"
     CDS             complement(6823..7728)
                     /locus_tag="KQD82_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4380960.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            8090..8824
                     /locus_tag="KQD82_002497"
     CDS             8090..8824
                     /locus_tag="KQD82_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380961.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            9035..10115
                     /locus_tag="KQD82_002498"
                     /pseudo
     CDS             9035..10115
                     /locus_tag="KQD82_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
     gene            10099..10671
                     /gene="mobB"
                     /locus_tag="KQD82_002499"
     CDS             10099..10671
                     /gene="mobB"
                     /locus_tag="KQD82_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG4380962.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            10790..11065
                     /locus_tag="KQD82_002500"
     CDS             10790..11065
                     /locus_tag="KQD82_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4380963.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            11308..13008
                     /locus_tag="KQD82_002501"
     CDS             11308..13008
                     /locus_tag="KQD82_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380964.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            13371..14558
                     /locus_tag="KQD82_002502"
     CDS             13371..14558
                     /locus_tag="KQD82_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380965.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            14618..15781
                     /locus_tag="KQD82_002503"
     CDS             14618..15781
                     /locus_tag="KQD82_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4380966.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDVLEIGTALYVQYAVDFLNK"
     gene            15932..16735
                     /gene="zupT"
                     /locus_tag="KQD82_002504"
     CDS             15932..16735
                     /gene="zupT"
                     /locus_tag="KQD82_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG4380967.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            16831..17391
                     /locus_tag="KQD82_002505"
     CDS             16831..17391
                     /locus_tag="KQD82_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG4380968.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            17641..>18042
                     /gene="rgbR"
                     /locus_tag="KQD82_002506"
     CDS             17641..>18042
                     /gene="rgbR"
                     /locus_tag="KQD82_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG4380969.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIK"
BASE COUNT         7065 a         1871 c         3116 g         5990 t
ORIGIN      
        1 ttttggtaca tttaatatta attattttca ttaacctttt gtattataca atataattat
       61 accatataaa aactgttttt tgtcgaatgt atgtttgaaa aaataaaaat tagtaaatta
      121 tcttaaaaat aattagatat actttggaag caataagaat attaacaata agataaatcg
      181 ttgaaatacc aacatgtgtt aaataataac aataaacaaa taataaaatt ttagatttca
      241 gaatatgaaa gaaccaaaaa acgattaact agtaatctta attaaattca tttagttgat
      301 gttaaagtat gaaaactata gtagaagttg taataataaa acgaacgaaa attatcatac
      361 taaaacataa tacatgctat aattgtatta gataaatgct tgaatatata ccaaaagtac
      421 tctttttata agagtgctta tttttttgaa attcatcaac atataaacta tcaagaatat
      481 tactcaacac accttaaaat tgatttaaat tctttttcat acacaaagtt atatgatata
      541 ataaaaaagt aaatatgtaa ccccaacaca tctttactaa gtcaaacatt attatataga
      601 gcattcttca ttatggagag tgctttttca tttctttgaa taatcatgtt gattatttat
      661 aatattttca tttaatttat ctttatctat caaatttaat gcacaattaa tacaaatgtt
      721 agttttcata tgacttttct tatgaaaagt aatatactta ttatcatctt tatttattcc
      781 tttattacag taatcacata atatagtctt agtcatattt ttattccttt cattttatat
      841 tttccataat taagtttaac atatttagta tatacctatg tatatatatt taaaacaatg
      901 ttttttattg caatatagaa tatattgtat aaataaaaaa atggagaata gatatttgat
      961 aattgtaaaa ttatgttata attaaaatgc aagaatatat cttgcaagag gctacaatct
     1021 aagggcgaaa gttttcggaa atcaccttcg aacgaaaggg ggtgagtata tgaaaacact
     1081 tatgtttata cttgttatag cgtattacat tactattgta ataaaatcta ttacaacaat
     1141 agtaaagtct ataacaagat tattagattc tataagtaga ttaaaagaat ctttacataa
     1201 actaagaaat cgtcctgacg gcaatcaaga cgatttcaat gaataaatca ttacttaaat
     1261 tattttgaat ttgaaacttc gctctaggct aatagattgt agtttctttt tttgtttttg
     1321 atacgaattt acatctttat tatatcgcat tttaagaaaa aataaaactg tgaatgctag
     1381 aaatatattt tattttatct ctaagtatat atattattag ccacctaaaa aactttcata
     1441 aaaacaaaaa gaacaatatc tttttggcaa ctggctggca taatttataa gaattttata
     1501 aatcttagtc cctatagctt tgcgtcacta aatttctcta gttttgccga tttagtttta
     1561 ttctacaact aaaataatac aatagaatta gttattattc aacaggatag tttgaaaatt
     1621 gaataattct atagatatga aagttatttt tcttctaaga aaacaaacta aaacagtaga
     1681 atatttggta aaaattacct atagtttttg tcttaaaaag ctcttattat taacttgaac
     1741 tagaacaatc gaaggagtga cataagttga aagaagaaat attagtagaa aaattattaa
     1801 gagcagaatt aatagtaatg gagtatttat ggaaaaaaga ttctctagtg actaaaaaag
     1861 aaattgtaaa agaaatgaga caggtttata gatggcataa aagtacaata aaaatactac
     1921 taaaaaggct agttgacaaa ggttatttgg ccagagatat tataaagttt caatctcatt
     1981 ataaaataat aatagataac aaagaatatt atgcttttaa gaaaaaagtg ttaaaatcta
     2041 gcaaaagcag aaaaataatg cgttctctta ctactacaca taagtctata agtaaagaaa
     2101 aactagacgc attagaagaa tattatagaa atttagaaga ataatttcga gatggaaaaa
     2161 attggatggt aatatttagt gtgattctct tgtttggaaa actttattga gatagaattt
     2221 aacatataca taattcgata aaggggtgtg actatgaaaa ttagtaaatt accagaagca
     2281 gaattaaaag ttatgagata tatttgggaa gctaacgatg tattaacgtc tagggaatta
     2341 gttgccgcaa tggaagaaaa atacgaatgg aatgaatcaa ctacattcac agttttaaaa
     2401 aggttagagc gaagggagtt tttaagtaca gagaaaatag gtaaacttac acactataat
     2461 gcaatggtaa aagagaaaaa atatctaaga tttgaaacaa aggaattttt gaagaacata
     2521 cataagaatt caatttcaag tttaatatca gcgttacata gtaaagatga tgaagtggat
     2581 gaagataaat taattgaact cgaagaacat tttaaaaatt taaaagagaa agaataaata
     2641 aagattattt taggtggcaa attatttttg ccaccttttt tgccaccatg acagtttaat
     2701 aaaataaaat acaaataaag aaatataaat aaaatagaat aaagaaattg ttttagatag
     2761 gttgtaaata aatgtactca taaaaacata aataaatata aataaacata aataaaatat
     2821 aagaatttct gtgagaaatg cacttaatga acttgtaaaa gaagatatat tatgtaaaaa
     2881 acgtggtaaa gggacttatg taacaatacc agagagaata gaagcaacat gtgcaggaaa
     2941 tagttttact aattcttgtc atcgtattaa tgcaaaacca agtaccaaaa taatctctgt
     3001 tttaataaaa aaagcagata agcaagtagc tgaagcttta agtattgaac tagaagagaa
     3061 ggtaatatgt attaaaagac ttagattaat agatgaagta cctgttattt ttgaagttga
     3121 ctattttaga atagattaca tgtttttatt aaaagaagaa ttagaagata aatctttaat
     3181 ggaggttata tctaataata taagcacatt acctaaacga gtagaaaata tatttgaagt
     3241 aaaacattca aacaaagaat attctgacca tcttaaatgt gcaagtaata tgccactatt
     3301 gaaagtaaga caatcggtgt atacagaaaa taatgatgtc ttatattaca atgagcaatt
     3361 tataagaagt gataagtaca aatatgcagt atcagcagaa atataatcta taatttagta
     3421 ggaggatgag tatgaaaaag ataatattag catctcatgg agacttttca aaagggcttt
     3481 taaatgcagt aaaaatgata gtaggagatt tagcagattg tgtcagttcg tatggtttgt
     3541 accctggaca aagtgcttca gattttgctt tagaattaga aaaaacaata tcagaagata
     3601 aagaatgtga atatataatt ttatctgact tatatggagc aagtgtatgt acagcaatgt
     3661 taagattaac taatctacat aatgttaggt tattttctgg aatgaatttg aatatggtat
     3721 tagaacttct tacaaggttt tctgatgaat taacagttga agatatagac caattagtgg
     3781 aagaatctag aagagggata caaagcgtga cattacaaat taaagaagaa gaagaggtat
     3841 tttagtttat gaaaaaaaag ggaatattat cagtatttaa aagtaaaaaa ccaattatag
     3901 caatgataca cttaaaagga gatactccag aagatatctt tgaaagagct aaaaaagaga
     3961 ttacaatctt tgaagaaaat ggtgtagatg gaataatgtt agaaaattac tatggtaatt
     4021 attatgattt agaaagaata cttgaatatg tatcaaaagc taatttatca ataccatatg
     4081 gagtaaactg tttaaatgtt gatactatgg gatttgaact ggcaacaaaa tataatgcta
     4141 gttatataca ggttgattct gtagtaggtc atgttaaacc tagagatgaa gctactttag
     4201 aagagttttt taaattacaa cgttcaaaat gcccagcgta tttaattgga ggcgtacgtt
     4261 ttaaatatca gccagtatta tcagaaaatg atgttgaaga agatttaaaa ataggaatga
     4321 caagatgtga tgctatagca gttacagaaa atgctacagg tcaagaaaca tcaatggaaa
     4381 aaatagaact gtttcgtaaa aatttaggag acttcccatt ggttatagct gctggagtta
     4441 cattggaaaa tgcaaaaaaa cagcttaaat taggagatat ggcaataata ggaagttact
     4501 ttaaagataa ttacaaagat tttggggatg ttagtgtaga acacgtaaaa acattcatgg
     4561 atgaaatcaa aaaaattagg gaggaattat aatgattaaa ttagtaagag tagaccacag
     4621 acttatacat ggacaagtag catttacatg gacaaagttt ttaagtacag actgtatatt
     4681 gatagcaagt gatgacttat taaaagatga attaagaatg gcaggactta aaatggctaa
     4741 accatctaat gttaagttag taatgaaaag catagcagat tctataaaag cacttaactc
     4801 aggtgttact gataaataca acttattgat actttgtgaa tctgtagaag atgtttacag
     4861 acttgctaaa gaggtaaaag ctattaaatc tataaacctt ggtggaacaa aatcagatga
     4921 taatcgtgaa aatatatcta aagcagtgca tgtatcaaaa gatgatataa agatgattaa
     4981 agagttagat tcagaaggcg taaatgtatt tgtacaatta gttcctgatg atgatgctac
     5041 aaatgtaatg aaattaatat aattttaggg ggatgaaaat ggaatttaca caagttattt
     5101 tgataactct aattgcattc tttgcatata tgcatagttt tgtaggttct acaatgcata
     5161 atagacctat agttgttgca ccacttgttg gtttagcact aggtaatctt catacaggta
     5221 tagtaatagg ttcaacatta gaattagtat ttatgggtgc atttcctgtt ggggcaagta
     5281 atcctcctga ttttgtgtct ggttctatta ttgccacagc gtttgtaata ttaacgggtc
     5341 aagatgtatc agcagcagtt gtacttgcag ttcctatagc aactctagta ttgcttatag
     5401 ataacttctt aatgacagta gtacttacat ggggagctca tattgctgac cgttatgcag
     5461 aagaggggaa tattgaaggc gtagaacgtg tgcaattatt atttggtata ggaaataaat
     5521 taatattagc aataatagta ggtataggat tttcattagg tgttccagta atagaaaaaa
     5581 tactttcatt tataccatca tatgtaactc atggaatgga tgttgcagca ggcgtgattc
     5641 cagcaatagg ttttgccatg ttagctagaa tgatgcttaa taagaaaaca gtagcatttt
     5701 tattattagg atttatattg gttgcttacc taaacataac agtaacagga gttgcattgt
     5761 ttggattagc aatagcttta atatatgtta actttgtagg agagaaggag gtaatagtag
     5821 atgacaacga gttctaagaa gttagagaca atttcaccag atagtaaaat aacgaggaaa
     5881 gacttttgga aatgttttag aaggtcattg acattagatt catcttggaa ctacgaacgt
     5941 atgcaaaata tagcatatgc atacatgatg gcaccaataa tccgtagatt atataaagat
     6001 gataaagaaa agaaatctaa ggctttaaaa agacatttag aatttatgtc agttactcct
     6061 catataagta ctcttttggt aggtatatct ggagctatgg aagaagaaaa tgcaaagaat
     6121 aaagaatttg atgctaatag tataaatgct gttaaatcaa gtttaatggg tccagtttct
     6181 gggataggag attcattttt ctggggaaca ttaaagctta tagcagctgg ggttggtata
     6241 gctttagctt ctcagggtaa cataatggga ccgattttat tccttttaat aataaatgtt
     6301 cctcatttta ttatacgcta tatatgtcta gataaaggat ttaagtatgg aactcaattc
     6361 tttaaagatg tgagtggttc aagtatagtt tcaaaagtta tggaagcagc ttcaatgtta
     6421 gggcttatgg taattggagg tatgactgca tctaatgtaa tgttaaaact tagtgttaat
     6481 gtaggtagtg gagaatgggc agagcctatt caaacttact tagaccaaat aatgccatgt
     6541 atgcttccag ctatgatatt cggtattatg tattggttat taggaaaaaa ggtaaagaca
     6601 actacgatat taatttcagt tatgattatt tgtatagtat tggctgctat tggagtagta
     6661 taaataaaaa aatcccaagt tcatttgaac gagggatttt ttgactaaat taaataaaaa
     6721 agaaagatgg ttcataataa tttataagca tttatgtgat agttataatt agcttattaa
     6781 ttagttataa actcaaaatt aagcactact ttattaaagt tattatatta taatttcgaa
     6841 atactcacat aatctgttta gaaatttatc ttctaatggg gaaaaagttt tctttttaga
     6901 gtaaatgaag aaaaactttc tagtaaaatc aacatccttt attttataac attttatttt
     6961 acctgcattg agataatcaa tagctgaaat gtacgatata aaggatactc caagacctaa
     7021 tctaaccatt tccttaatag cctcatttga ttctacatga gcgattacat ttaatttact
     7081 tactggaaag ttatttttac taagtgtgtt tagtattaag tttcttgtac ctgacccttc
     7141 ttttctcata ataaaattta gataagccaa ttctcctata tcgatacaat tatttttatt
     7201 atcaatttta aaatctgaag gagtgataag aacaagttca tcatctaata agtctaggta
     7261 ctctatttga ggattattga ttttagaacc aactaatcca aagcttattc gttcatttaa
     7321 aatttctgat atagcatatt gagaatcata atggcttatg gaaaatttta catctggata
     7381 actcatagaa aaactcttca taaaatcagg taaaatataa gtttcaggta tggaactaca
     7441 tgctatatct atgatacctt ctatttttcc tgaatattct tttatatcat aaattgcacg
     7501 tttgcaatta ttcaatatat atatagcatg ttcataaaga atttcaccag attttgtgag
     7561 tgtaataact ttgttgcttc tatttataag aacagtttct agctctctct ctaaattctg
     7621 tatgtgtgcg cttacagtag gttgagttaa aaaaagttct cttgcagcct ttgaaaaact
     7681 ctgatgtttt gcaacagcaa caaatacttc aagttgttta aaatccatta tttaatcctc
     7741 cttaaattat ctaaaactat tataacatat ttgataaatt aatagtcaaa ggtgattttt
     7801 aaaatagaaa acctttaaat attcatggag tttttgattg tttaggaata atttggtata
     7861 atgttgtaag gtacttacaa taaaaatatc gtaaattgaa ttttcaaaat tcaactttaa
     7921 agtgtttttt attacagaaa aattacaatt agcttcttaa tcttattaaa ttcaaataaa
     7981 tattatataa ttattttata atataataaa tataattatt gaaaaaagag ataactgtaa
     8041 attgagatat agataaatac tataaaattt aagaaaggag gattgattta tgaagttaaa
     8101 aaggtcatta gtttgtgtaa ctattttagg tattatactt gttggatgtc ataaagaaaa
     8161 tactaaagag aaaaatcaag tagcaagtaa agcaacacag caaaaaacta tgacaaaagt
     8221 tcaaaatgat gttaatgaaa ttatgaataa agattataag tacattataa agaatatggg
     8281 tataccttat aatacatttt attatataaa acctaaagtt ttaaaagaat caaatacaat
     8341 gcaagatata aatacatcaa gttatatgac attagtttat ccaaaatata ctggaaatga
     8401 tgaactagat ggaagtgctt tatatgttga tattaacgaa aataaagttg tcaatgttga
     8461 aaccaattct ttttcatcac aaggtatatc agtcattgat gctgaatcta gtatagtgat
     8521 tgaaaaaagt gaccatgaaa aatcggctgt atcactagaa aattttagac atattgacct
     8581 gggagagtat gttggtgttg aagattccag aataaatgaa attgtaggag atgcgaatta
     8641 tgatttaaca gcatataatc atgaaggcag taaagtagtt aagagttata gattgaaaga
     8701 agataacaaa atattaaaaa aagaggtact tacaataagt attgtggata acaaaataaa
     8761 atctataaaa actattgaaa gtgataaaat tgtaaaaata ataaaaggaa ctttattaga
     8821 ataaaatatt ctaaagcata tgatgctgtt tcaagataga aggaaatttc tagctgggaa
     8881 cagttttttt attagattta taaaatttga actgcactta tagataacta ttagttataa
     8941 ttaaatataa gataaaagta tttccaactt ttataatcta taatatatta catagaaata
     9001 tcttaaataa aaaatatatt ggggagtatt taaaatgata gaattagaag atgccataaa
     9061 aattattgaa aaaaatgttt taccaataaa cagggtcaag atagtaaatc ttatagatgc
     9121 agtaaataag gtagttgcag aagatatata ttcaacaatt gatagtcctc catttgatag
     9181 gtcaccctat gatggttatg catataatgc aaattctgaa aatagaaatt taaaggttat
     9241 agggactttg tatgcaggag atgtatttaa tagggtatta aataaaaatg aagcagtgaa
     9301 gataatgact ggtggaaaga taccacaggg tgcaaattgt gtaataaaaa aagaagatgt
     9361 aacagttata ggtgatatga taactttaaa tataaaatta aaggaatacg ataattacat
     9421 atttaagggt gaggatataa aaaaggggca attgattatt aataaaaatc agaatatagg
     9481 atatgatggt attggagtat tagcaagtat tggaatatcc aaagtaactg tttatgatga
     9541 tttaaaagtt ggaattttga atacaggtac agaactacaa gatataaatg agactttaga
     9601 gaatggtaaa atatacgata gtaatagata taccttatat tcaagattat taaaacttgg
     9661 aatacaagca gatacaatat gcaattgttc tgatgatata ttggagttag aaaaaaaggt
     9721 aaagaatatg ttacagagtg tagatttttt aattaccaca ggtggtgttt ctgttggaga
     9781 taaagattta attccagatg tattcaaaaa aataggggca acagaattat tttggaagat
     9841 aaacattaaa cctggtggag cttgttatgt aggtacattg ggagaaaagc tattatttgg
     9901 tctttctggt aatccacatg cagcaatttt caaataaaga tagatttgta agtggaaaac
     9961 tatatacaag tgaaggtaaa ttgtatgtag agctaaatga taagaaagat gcaaaagcta
    10021 gattatctgc aactctaaga agtaattgta tgattaagat taaaaagaat gagacaataa
    10081 aagaaaatca attagtggat gttgttatta gatagtttta tgaaaggaca caattttatg
    10141 tataatatat tgtcagttgt ctcatattca ggagtaggta aaactacgtt gattgaaggc
    10201 ataataaaag aattaaataa aaaagggtat cgtgttggag ttattaaaca tacctgtcat
    10261 gattttgata tggatgagga aggaaaagat acttataaac atagaaaatc tggggcgaga
    10321 aaagtctgta taatatcaga taaacgagta gcatatattg aagaactaaa agagaagaat
    10381 ccattatata aaatgattca attatatgaa gatatggact tgattatcat agaaggatat
    10441 aaaaattata gatttaaaag attggaagta actagaaaag gtaagtatga ggatatttta
    10501 tctaataagt ctgatttgat aggtattgtc agtgatatag aatataaatt gaatattgaa
    10561 cagtttaatt taaatgatta taaaaatata tcaaagtaca tagagtcttg tataaaaaat
    10621 gaaattttaa gtataagcaa tgttgaaata aaaaacatat taaaagaata attttaagat
    10681 gttcttctta tataaaatga tataattatt tatataaata aagatataag aaatttatca
    10741 attttatttt gagttataaa atattaaaga aattgtagga ggacaatata tgaaaaaaat
    10801 attagtagcc tgtggagcag gaatcgcaac atcaacaata gtttgtgaca gagtagaaag
    10861 attggttaaa gaaaataata tacaagcaga agttgagcaa tgtaaaattg ctgaatgttc
    10921 aacaaaacaa gaaggtgctg atttaatcgt atctactact atactaccaa caacttatga
    10981 tatacctaca ataaaggcaa caggatatat aacaggtata aatacaacag cattagataa
    11041 aaagatatta aatgcattga agtagttttg tatttataaa aaaaatttag tattaaatat
    11101 agagttgtat ctttataaaa ttttaaatat agagatatag ctctttttat gttgtttcaa
    11161 gtatacaact atatgatata aatcttcaat tttatgaaaa agattgttat tactttgtaa
    11221 gtttataaaa gatttaaagt tattgttatg ttatactaat ttatgggtat tttttagaag
    11281 tactaataaa ggagggattt aatacttatg ataaaacaat ttgtcaaata ttataagcca
    11341 tataagaaaa tttttacact agaccttata gcagcttttc tattttcctt atgtgattta
    11401 gtttatccta tgataactag aaatataatg gatgacgttg ttccaaataa aaacttaaga
    11461 atgttagtag tttttgcagt agcacttata cttatattta tagcaaaagc agggcttaac
    11521 tattttatgc agtattgggg acatgttatt ggtgtagata tgcaagctga tatgagaaat
    11581 gaggtattta cacacttaca aagacttcca aatacgtatt ttgataataa caaatctgga
    11641 gtaactatgt ctagaatagt aaatgattta atggatataa cagaacttgc acatcatggg
    11701 cctgaagatt tattcatttc tatagtaatg ttggttggtt cattctttat acttatagat
    11761 ataaatattc ctcttacgct tataatattt gctatattac cgtttataat ttggtttgca
    11821 atagctaaaa aagataagat gaatattgct tttatgaaga gtagagttac tataggtgat
    11881 gtaaatgcta ctttagaaaa tagtatagca ggtatgaagg taactaagtc tttttgtact
    11941 gagaaagaag agttaaataa gtttgtaaga agcaataaat tatttagaag agcaagacaa
    12001 gattcttata aggtaatggc agaatattac tctggtatga atttatacat ggatatctta
    12061 gagtgggtag ttgtaatagc tggtggttat tttacttata taggaaagat tactttgggt
    12121 gattttgctg cttatatact atatgtaaaa atgtttatac aacctatgaa aaaattaata
    12181 aactttacag agcaatatca aaatggtatg acaggtttta aaagattcat agagataatg
    12241 gaacaagacc atcaaaaaga agctaaaaat cctattgaac ttgaaaatgt aaaaggtgac
    12301 attgagatag aaaatatatc tttcacatat gaagataaga ctcaagtttt ggataattta
    12361 agtttatcta taaaagctgg taagactata gcattagttg gaccttctgg aggaggaaag
    12421 actacattat gtaatctatt gccaagattt tatgaatttg ataagggtga tataaaaatt
    12481 gatggaaaga gtataaaaga tgttagttta aaatcattga gaaaaaatat aggtatagtt
    12541 caacaagatg tatttttatt tacaggaact ataagagata atattctttg tgggaaccct
    12601 aatgctacag atgaagaaat gatagctgct gctaaaaaag ctagaataca tgactttgta
    12661 gagactttgc cagatggata tgatacttat ataggagaaa gaggcgtaaa attatcaggt
    12721 gggcaaaaac aaagaatttc aatttcaaga atctttttaa agaatcctcc aataataata
    12781 ttggatgagg ctacatctgc tttagacaat gtaacagaaa gagaaattca agagtcttta
    12841 gaggaattaa gtaaagatag aacaaattta gttgttgcac atagattgac tactataaaa
    12901 aatgctgatg aaatcatagt attgacggat aaaggaattg aagaaagagg tactcatgaa
    12961 gagctagtaa acaaaaatgg agtatatagt aggttacaca ataactagta tacaaatttt
    13021 acaccatagc taacatacac attaaactta caaacatagg atatattaag attcataaag
    13081 acaattcatt ttatatttat aagatgtttt atgaataaaa tgtatcctat gttttatttt
    13141 ttgcaaatta catttaattc tctttgtaag aatagagtta aatagccaat ggtcgggata
    13201 acgtttttac aaaaagaata atttagaaaa ctaactttaa tttatggaat ttatcgaatt
    13261 ttatgaaaaa aatcgaattt tgacttgaaa tgtttttcaa aagtagtaaa ataatggata
    13321 actatacata cattttataa aaatatattt attatgaggg gggattttgt atggaggcaa
    13381 taacagcttt ttgtctagtt ttagtatcac tgacgatagg agatatagtt tcagcaaaaa
    13441 ctaaagcttt tgttccgtcg gtttttgtat cagcaattat atttatagta ggattttgga
    13501 cttttttccc agaaaatatt gtggacttag ccgctttagg aacaccatta gctcaattag
    13561 gtatgctttt gttaataact catatgggaa ctatgatgag tattaaggaa ttagcaggtc
    13621 aatggaaaac aatagtaata gccttggcag gaatagtagg aatatgtgta ggtgctttgg
    13681 cattagggac agtagtattt ggatgggata cagcagtaat agctacacct cctcttactg
    13741 gagggctagt tgcatctata atgatggcag atgctgctac agcaaaaggt cttgcaagtt
    13801 tatcagtact tgcaattttg atgtatgttg cacaaggatt tgcgggttat cctattacag
    13861 cattaatgct taaaaaagaa ggaaaaagac ttttatcaga ctttagaagt ggaaaagtaa
    13921 ctgtaaatac agatgaagaa aaagttaagg atttacctga acaaaaaagc agatttaaga
    13981 taataccaga cttaccagaa aaatatgata cgacatatat gataatactt agattaggat
    14041 ttgtagcatg gttagcagta caatttacaa atcttacaaa tgaagttgta tcaagatatg
    14101 ttatatgttt aatatttggt gtaatagcat cagaacttgg aatagttgat agaaaaccac
    14161 ttaataaatc tggttcattt ggatggttaa tgacatcatt gatggctttt atatttgcag
    14221 ggttagcaaa agctacacca gcaatgcttg ttgaaatggc agttcctctt gtaggaatca
    14281 taatatttgg tgtaataggt atgggaatat tctctattat agtaggtaag cttttaggtg
    14341 aaagtaaaga aatggctttt tcagtttcgc ttacatcact ttatggtttt ccaccaaact
    14401 atatattaac tgaagaggca gtaaaggctt tggctgaaac accagaagaa aaagaatttt
    14461 taatggataa gatgcttcca aagatgcttg ttggaggatt tactacagtt acaatagctt
    14521 ctgtagtaat agcaggtatc tttgtaaatc taatatagta tttgagtgat aaaaagtatt
    14581 ttaaataaga agaatgtgta ctacttggag gtatggaatg gatattaaag aaataacgaa
    14641 gagttataaa gactatgtaa ttaagcttag aagggaattt catgaaaatc ctgagaaaag
    14701 tatggaagaa gttagaacat caaagagagt taaagaagaa cttgataaaa tagggatacc
    14761 atatgtatca gctggaggta caggtgtaat tgcaactata aaaggagcta atccaggaaa
    14821 gactgtagca cttaggggtg atatggatgc tctacaagtt gttgaatgca ctgatgtaga
    14881 atacaaatct aaaaatgaag gacttatgca tgcttgtggt catgatggtc acacatcaat
    14941 gcttctaggg gcagcaaaag tgttaaatga tattaaggat agtatcaatg gaactgtaaa
    15001 attatttttc cagccaggag aagaagttgg taaaggtgca agagctatga ttcaagatgg
    15061 tgctatggaa ggtgtagata gtgtgtttgg aatacatctt tggactgatg tagaatctgg
    15121 gaccatatcg gtagaagaag gaccaagaat ggcttctgct gatttcttta aaattactgt
    15181 aaaaggtaga ggtggtcatg gttcacttcc tcatcaaggt gttgatgcag tgcttgcaag
    15241 ttcagcaata gttatgaatc tacaatctat ggttagtaga gaagtaagtc ctctggaacc
    15301 attagttgtg agtgttggag ttttaaattc tggaactaga tttaatgtaa ttgcgagtga
    15361 agctatctta gaaggtacaa taaggctatt taatccagag cttagaaagc aaattcctgg
    15421 aattttagaa cgtatagcaa aatctacagc agaagcatat agagcagatg ctgaattaga
    15481 gtatgggtac ttaacaccag cagttataaa tgacaaagaa tgttcaaaga tagctacaga
    15541 tgcagcaatt aagctatttg gagaagactg tataacacta tttgaaaaag taacaggagc
    15601 agaagattta gctgagttta tgaatatagc acctggtgca ctagcatttg taggagctag
    15661 aaatgagtca aaaggtgctt gttatcctca tcatcatgga tgctttaata tagatgaaga
    15721 tgttctagaa attggaactg ctttatacgt gcaatatgca gtggatttct taaataaata
    15781 attttaatat actataaaga aaaagcagag actaagttta tatctctgct tttttattgt
    15841 gtatattttt tataaatatt gactatataa aatatatatg atagcataaa gaaaatgata
    15901 atcaatatca attaaaaaga aggtgattta tttgaataat gtaatatttg catttttgat
    15961 aacattattg gctggtttat ctactggtat aggaagttgt atagcatttt ttgctaaaaa
    16021 aacaaatact aagtttcttt ctataagttt aggattttct gctggtgtta tgatttatgt
    16081 ttctatgata gaaatttttc ctaaagcaca agatgctttg acaaaatcta tgggagagaa
    16141 attaggaagt tggtcaactg taatagcttt ttttataggt atggctatca ttgctgttat
    16201 tgataagcta attccacaag aagaaaatcc acatgaaata aagaaaatgg agaatataga
    16261 agaaaagaat atcaaaaaaa ataagtctct tttgagaaca ggtatattta cagcaatggc
    16321 tatagcaatt cacaattttc ctgaaggatt agctacattt atatcagctc ttgatgatgt
    16381 tacaatagct attccgatag caattgcaat tgcaatacac aatataccag aaggtatatc
    16441 tgtatctgta ccagtttatt atgccacagg agataaaaag aaagcttttt attattcctt
    16501 tttatctggt atgtctgagc ctttaggagc gattattgga tatgtacttc ttagaaattt
    16561 tttaaatgat ataactcttg gaattgtatt tgcaattgta ggaggaatta tggtattcat
    16621 atcactagat gaattattgc catcagctag agagtacggg gaacatcatt tatctattta
    16681 tggattgatt gcaggaatgg gtgttatggc tattagttta ttactattta aatagctttt
    16741 taaatatatg attgtaacct aaaattagta tcaaaatgat ataattattt aaatagattt
    16801 ttaagctatt taaaaatatc attttggaga atgagtgcta tggaaaagaa aataaaattc
    16861 aaatttgact ggtatagttc acctgataag atagagaaat ggttagaaga tattgaagct
    16921 aaaggatata atttatataa gataaataaa tttggaggaa tattttattt tcataaggga
    16981 agtccaagaa aaataaaata cactgttgat tactactatg atgtagagaa taattatttt
    17041 gatgaacata aatcaaatgg ctggaatctt gtatttagga gtattggtag ttttatgggt
    17101 acatctgcat ggtatatatg gtcgaaagaa tacaaagaca tacctcctaa attatatagt
    17161 gattctgata aaacaaataa agtaaagtca attttattta tgaatatgtt tatattcttt
    17221 atgttagtag taatatttat atatttattg aatattgaaa taagaaattt atatagtggt
    17281 tatggagcat ctgtaactgc tattgtacta cagattatat tgttgatttt atatgcagta
    17341 caattatata ggagattaat gtattattta aggcttaaaa agtttaaata aataaaagaa
    17401 ggtgtcctaa aattattttt ttaatatttt aagacatctt cttttgtcag taagtactgt
    17461 ttttaattat agtaatttct gtctttaaat tgaaatatca aataatagtt gtgtgggatt
    17521 gagtattgat aaaaataatt taaaaatgtt atcctaaagt atcagatata caaaattatc
    17581 tttaaatata ttataaatat tttaattgta ttaactagaa attatgtaag gaggcatttt
    17641 atgattaaga tagcagtttg tgaagatgaa aaagaaacac aacttttaat agaagattac
    17701 cttgagaata tattaaaaga tataagtata gaatatgaga tacaaaagta tatatctgga
    17761 gaagagttac tggaaagtaa tttaaaagat atagatatat tacttcttga tataaaaatg
    17821 gaaaaactaa atggaatgga tactgcaaga aagattagag aagtagacaa tgaaatggaa
    17881 ataatatttg taacttcatt aatagattat gtgcaagaag gttatgaggt aagagcttat
    17941 aggtatttac taaagccaat agagttggaa gaactaaaaa aacatgtgct aacttgtatc
    18001 aaagatattg aaataaataa ggaaagtcat attacaataa aa
//
LOCUS       DAENCV010000074        18022 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAENCV010000074 DAENCV010000000
VERSION     DAENCV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.075"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            78..413
                     /locus_tag="KQD82_002507"
     CDS             78..413
                     /locus_tag="KQD82_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4380970.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            550..1827
                     /locus_tag="KQD82_002508"
     CDS             550..1827
                     /locus_tag="KQD82_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG4380971.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIRSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1948..3336
                     /locus_tag="KQD82_002509"
     CDS             1948..3336
                     /locus_tag="KQD82_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG4380972.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3707..4420
                     /locus_tag="KQD82_002510"
     CDS             3707..4420
                     /locus_tag="KQD82_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG4380973.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4538..5407
                     /locus_tag="KQD82_002511"
     CDS             4538..5407
                     /locus_tag="KQD82_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG4380974.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5562..6707)
                     /locus_tag="KQD82_002512"
     CDS             complement(5562..6707)
                     /locus_tag="KQD82_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG4380975.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7041..7817)
                     /locus_tag="KQD82_002513"
     CDS             complement(7041..7817)
                     /locus_tag="KQD82_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG4380976.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8399..9373
                     /locus_tag="KQD82_002514"
     CDS             8399..9373
                     /locus_tag="KQD82_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG4380977.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9471..9776
                     /locus_tag="KQD82_002515"
     CDS             9471..9776
                     /locus_tag="KQD82_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG4380978.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9932..11092
                     /locus_tag="KQD82_002516"
     CDS             9932..11092
                     /locus_tag="KQD82_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG4380979.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11142..12044
                     /locus_tag="KQD82_002517"
     CDS             11142..12044
                     /locus_tag="KQD82_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG4380980.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12124..13017
                     /locus_tag="KQD82_002518"
     CDS             12124..13017
                     /locus_tag="KQD82_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG4380981.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13070..13251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13479..14609
                     /locus_tag="KQD82_002519"
     CDS             13479..14609
                     /locus_tag="KQD82_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4380982.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14681..15706
                     /locus_tag="KQD82_002520"
     CDS             14681..15706
                     /locus_tag="KQD82_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG4380983.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15709..16905
                     /locus_tag="KQD82_002521"
     CDS             15709..16905
                     /locus_tag="KQD82_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4380984.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17033..17758)
                     /locus_tag="KQD82_002522"
     CDS             complement(17033..17758)
                     /locus_tag="KQD82_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG4380985.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6934 a         1999 c         3113 g         5976 t
ORIGIN      
        1 gtatatttat aatattaata aatatattgc tataaaagtt tgtttaataa attaaataaa
       61 gaaaagagga gaataaaatg tacaacatag atgatatgat atataattgt ccagtagaag
      121 ctttatcaaa tattttaggt aaaaaatggg tggctataat tatatggaaa atacaagaag
      181 ataaaaaacg atttggagag ctacaaagag ctataccaga ctgtagtaag aaaatgttag
      241 ttcaacaact agatttttta attgaacagg ggattatagt caaccaaaaa aagttggtta
      301 ataatgttgt agagtcaaca tattttttaa gtgaatcagg attacgattg cttcctgtca
      361 tggaaaaaat gattatgtgg agcaataaaa atttaaattg tgataaatac taaaatttac
      421 tctgtaagta atgaaaattt aattaagcta tgagtagaaa aatgtattaa aattacataa
      481 ttttttagtt agattttaat ttagaaatag gattgtaata ttggaaataa aataaggagg
      541 aattttaatt tgcaaaatgt aaatttattt ttagtattta tagaaggtat agtatcattc
      601 ttttcaccat gtatactgcc aattttacct atttatttga gtatattgtc aaatagtagt
      661 gtagaaaatt taaaagaagg aaagacgagt tttataggaa gttctttatt taaaaatact
      721 atattttttg ctcttggaat ctcaacaaca ttctttatat tgggttcatc agtaaaagta
      781 ttgagtatgt tttttaatga aaataaagat ttaataatgt ttattggtgg gattataatt
      841 attatcatgg gattatttta catgggaata attaaatctt caatattgaa tagagaaaaa
      901 agatttaatg ttaagtttaa agaaatgaaa gcaataacag cttttatatt aggttttacg
      961 tttagttttg gatggacacc ttgtattggt ccaatattgg catctgtatt agtcatggtt
     1021 tcaagttcaa gtaatcatct tagtgccaat cttttaatag cagtatatac tattggtttt
     1081 attttgccat tcataattac agctatgttt tacagtaaac tatttaaaac tatagataag
     1141 ataagatcta acatggaaat tattaaaaaa ataggtggga ttatacttat agtatcagga
     1201 atattaatga tggtaaatgg atttggaagt ataagcaaac actttaatac gtcacaaaat
     1261 agtaaaattg aaagcaagca agaagaaaat aagcgagaaa attctactga taaagaagaa
     1321 aattctgatg gtaatgatag tcaaaaagac tctaataatg acaataatga taaagggtct
     1381 aatgatgaag atagaataaa gtctattgat tttactttaa cagaccagta tggtaaaaca
     1441 cataaattaa gtgattatga aggcaaagtt gtattcttaa atttctgggc tacatggtgt
     1501 cctccatgca aagaagaaat gccttatata gaacaactgt ataaagacta taataaaaat
     1561 aatgatgatg tagtaatttt aggagtagct tcacctaatt taggtagaga aggttcaagg
     1621 gaacatgtag ttaacttctt aaaagaccaa ggttatacat tccctgttgt acttgatgaa
     1681 gatggagcat tagcatatca atatggaatt aatgcttttc caactacatt tataattgac
     1741 aaggaaggat atgtaactca gtatattcca ggagctatgg ataaggcaac tatggcatca
     1801 tttattgaaa atcagagaaa taaataaaag tatttaagta taagtttata aatcaattta
     1861 aggttacaaa aaagtgccta attgtaccac ctaaataatc ttgttatagt aaaagtataa
     1921 acaatacaag aaagggttga attttatatg aaaaaaacat ttgatgctat aataatagga
     1981 tttggaaaag gtggaaaaac attagcaggg gatttggcaa acaggggatt aaaagtagct
     2041 ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg taaatgtggc atgtatacca
     2101 acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa agaatataaa ttcttgggat
     2161 gaagtacaag ctgaatatga gaaagctata gataaaaaag aaactttaat tacaaaactc
     2221 agagaagcta actataataa attaaattca aatgagaatg taactatatt cacaggaatg
     2281 ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg aaaatgaaat ttatgagtta
     2341 gtagcagata atatatttat taatactggt tcaagaccat ttatacctaa tattaaggga
     2401 attgaaaata agaatatagt atatgatagt gagtctctta tgaatctaag aactttacct
     2461 aaaaaaatga caattatagg agctggattt ataggtttag aatttgctgg tatttatagt
     2521 tcatttggag cagaagttac aatattaaac tctaataatg gcattttacc aaatgaagat
     2581 gttgaggatt cagaagaaat aataaaatta cttgcaaaga gaaatgtaaa aattgttaat
     2641 aatgcaaata taaaagagat taaagaagtt tctgaattag caatagtaga atatgaagta
     2701 gatggtaaat ctaaagaatt aacaagtaat atgatacttg tagctacagg aagaaaagca
     2761 aacactgaag ggctagggct agaaaatgca ggtatagaat taaatgaaag aggatttatt
     2821 aaagtctcag aaactcttaa gactaataaa gagcatatat gggctatagg agatataaat
     2881 ggaggtccac aatttactta tatttcattg gatgattatc gcatagttat taatcagtta
     2941 tttggagata agactagaac aactaatgat agaaagaata tcccaaattc tatatttata
     3001 agtccagcat tttcaagagt tggtcttaat gttaagcaag ctaaagaaaa aggatatgaa
     3061 gtattagtgg caaagatgcc agtagaagca attccaagag ctaaacagat tggcaaggca
     3121 gatgggttta taaaaatagt gatagataaa aaatccaata agatacttgg agctagtatg
     3181 atatgtgaaa attcaagtga gattatccat cttattcaat tagctgtaga tttagaagta
     3241 gagtatacat atttaagaga ccgtgtttat gcacatccaa ctatgacaga agctcttaat
     3301 gatattctat ctcctaatat gataaaagaa gtataacata agtttaatat ataaacctaa
     3361 aattatcatt ataagtagat tttagaaaga ggctatctca aaaaatagag ataagtctct
     3421 tttttactgt ttaaatgata aaaatatatt tgattttata aaagaagatt atcttatgaa
     3481 ctaaaaagtt tattttaaaa cactacctat ttatgttata aacttataaa tataatatag
     3541 aatctattat tctgtattat taagtggggt taagcgttat aattaattat aaatattgaa
     3601 taatctaaat ggataagata atggtattaa aaattatctt aaaaggtgaa ataatctgta
     3661 tttaatgtag tacagttata tctttaaagc aaggtggtga aatgatataa ataagatatc
     3721 taaaagaggg tttgatgata gtgataaaag atggttttca agtaaagaat taacaagact
     3781 tataaaggct aaagaagaaa tagaatggtt attaaatagg gattacaaaa tagacccagt
     3841 cgttactttt gttggtgata gatatcagtt ttctataaga caaagagatg cattaaaaag
     3901 ggcagtctgt actgatgaaa aaaatattat aagacaatca aagaggcttt ctctagataa
     3961 aatcaaagaa gataccgtaa atatagatgg ctttaatttg ataatatcca tagaggtagc
     4021 attatctggt ggtactctag taataggaag tgatggcaat ataagagatt tagcaggtct
     4081 taggggaacc tataagatta tagataaaac agaagaagct ttgaatctga taggtaaatt
     4141 ttttaataaa tacaatgcac agaatcttaa attttattta gatgctccag tttctaattc
     4201 aggaaattta aagtatagga tattagagca tgccaaaact tggggaattg aaactgaagt
     4261 agaattagta aaaaatgcag atgttgtatt ggaaaaatta gatagagtag ttagtagtga
     4321 tgcagtcata gttgacaagt gtataagtta ttttaatgtg gctagaggta taattgaaga
     4381 gtatattaaa gaatgtaata tcataaattt aaataaatag ataaatatag gtgagaatat
     4441 ataagtatat gtagcaatat tattagtaat acaagtaaag tttgaaaata ggagaacgaa
     4501 tacatatcga atttcataaa atgttgtaaa aagacatatg agaggatgga tatgtgtgaa
     4561 attaactaaa ctaaatatta aaaaatatag agcacctata tataaatatg ctttagcaaa
     4621 tgtaaatttg aggtctgcta aatctaccaa ttcaagtatc ataacagtaa tacctcaggg
     4681 agctaaaatg gaagtattgg atgaagaaga tgactggatt aaggtgatgt acaattctca
     4741 agaaggatat gtgtataagg atttggtatc agtaagcgaa tatgcttgga gtaacttaaa
     4801 cttaagagaa gataaatcta caacttccaa tataattaca gtaattcctg aaaaatctag
     4861 ggttgaagtc cttcaagtag atggagattg gagtaaggtt gtatatgatg ataaaatagg
     4921 atatgttttt aattattttt tatcaattga tgggaataaa ccaaatgaat tagattataa
     4981 gtatttttat actgacatga ttaaatttgt taacgaaaat aatattaaaa gtactagcga
     5041 ttatctaatt gtaacagacc ttagaaataa atatacttat atttttaaga aagataatgg
     5101 aggctgggga caactttaca agtggcaatg tactataggt aagcctgaga ctccaactat
     5161 aacaggtata ttttatataa gtggaagaaa accttctttt ggaacggatg agtattcagt
     5221 taaatatgct actagaatta aaggtggata ttactatcat tctgtgctat atgattcaac
     5281 aggaagttat ataatagatg gcagacttgg tgaagctctt agtcatggat gtataagatt
     5341 aagtacggaa aatgcaaagt ggatatatga taatatacca gatacaacaa cagttataat
     5401 tcattaagaa aaattttgag ttttttattg ggataatgat tcttaaatta ataggattat
     5461 atattaactt ctcataaaga gtactattta aaaagtgtaa taagagaatg ctattaacaa
     5521 aacaaagata ttttgaggca ctctcttata aatatacata attaagatat tttcataaac
     5581 aattctataa tcttatcttt atcaaagaaa acaggattag caccagcaca agcatctttc
     5641 atagagtttt ctgcaagcaa ttcaaaatta ggattatcaa cacctacatc ttttaaagat
     5701 tttgggattc caacctcttc agaaagagct tttatcttat ctattacaaa gtcaacacac
     5761 tctttatctg atttattatt tacatccatt ccaataactt cagctattgg tcggaatttg
     5821 gcaggggctg atttagcatt ttcctcctca actattggaa gtagcatggc attacatact
     5881 ccatgaggta aatcatataa acctcctaat tgatgagcca tagcatgtac atttccaaga
     5941 cctgcattac taaaggcaat tccatttaaa aaacaagcat aacacatttg ttctctagct
     6001 tctatgtcat taccattttt aacagctctt ggaaggtatt taaatatctg ctttatagca
     6061 tataaggcag ttgaatctgt aacatcataa gcaccattag ctactacagc ttctatagca
     6121 tgagttagtg catccatacc agttgctgct gttaaattag atggtttgct aatcattaat
     6181 tctgggtcat ttactgtcat tgtagctagt gaattggtat caaccattat catctttaca
     6241 tgtctgtctt catcagttat aacatagttt attgtaactt cagcagatgt accagcagta
     6301 gtagttatag ctatgatagg aagacatttt ttagaagttt tgttgatacc ttcatagtct
     6361 ctaatatctc ctccattagt ggctaatata gagatagctt taccacagtc ttgtggagaa
     6421 ccacctccta tagttataac gaagtcgcaa ttttctgatt tcaatatttt aagtccttta
     6481 ttcacattag ttacagttgg attttgttta acttcatcat aaacaacaaa gtctacatct
     6541 atctgagata aaatatctgt tacttttttt acagtaccat ttgaaactag aaatttatca
     6601 ctaacaataa atgctttttt agttcctaat gatttaattg aatctttagc ttcattaaga
     6661 catcctttcc caagtaaatt tattggtggt acataataaa tgctcataac taaaatctcc
     6721 ttttattttc attgattttt atgtaaaaat atatatctaa caattttcac taatttactc
     6781 ataatatatc gtatcttaga ttgagatagg gttgctggtt cttatataag aacttaattc
     6841 gtaattacga ttatgattag aatttaacat agtattttta tcatgtcaat aaaaatataa
     6901 aaaatattat aaaaaaaaac aaagttactg tagttaaatc agcaactttg tttttatagt
     6961 ataaatcaaa taaatacatt agtttaaaac aaaattttta attattatgt tttgatatct
     7021 tccatataca gaatattaat ctaatttatt acaatgtctt cattacgttc ttccatcatt
     7081 gattgaatat ttttctgagc atttaagagt aattttttta tcagttctat tttttcgtca
     7141 ttagctctaa ataaaggcag tgttacacta acagcagctc ttatacatcc attttttctt
     7201 attggtacgg caagacattt taggtgttcc attgattcct cacactctga agctatttgt
     7261 gttctttgaa cttcaagaag ctggtcgtat aaaacatcaa aatctgtaat tgatttttct
     7321 gtaaaacctt ttagaccatc tggatataag cttattagtt tttccttgtc aaaatcacat
     7381 aaaagagctt ttccaagacc tgtacaatta gcaggtattt tttttccaac ataagaaata
     7441 agtctaattg gttctggaga atctactttt ccaatataaa gaacttcatt attgtcaagt
     7501 ataccaagct gacaagtttc agaacaggaa ttaactatat tagtcatttc gtcaattata
     7561 tagttaaaaa tagttctttt ttcaaaaaat gaagacccta atgtgtaaga acgtatccct
     7621 atagaatatt tggatgtgtt tttatcaata gtaatgtatt ttctagcatt aagtgtatga
     7681 actataggaa agatgctact ttttggagca ttgataacat ctgctatttc tgtaagagta
     7741 tatccatttt cggattctgc taatgcttct aaaatatcta atatcctagc tgttgggcga
     7801 tgattatctg aagtcataaa tttccccctc tttttattaa tttaaagtat tagaatatgt
     7861 tgattttagc acactatacg atattttaca atgtagaaaa taaatatgta aatttatata
     7921 taaataagct cttgatttag atacagatag atattttttt aggctgtaca ttaaggaata
     7981 actaatttcc aaaatatacc taaagaaagt aagtgatatt tagttatata atttaagtaa
     8041 atatatgcaa tagtgatttt tatatgtaaa tatcaaaata ttatgaactt atattttatc
     8101 tattttttac actgtataaa aactttatag ttttgaatac tatataattt taaatacgct
     8161 gtatttttgc atataaggaa gtcatttgat aatatacaag gtattatcag aaagtttgaa
     8221 aaaattttga caaaaattta ttttttttat tgactcataa ataggaaaat gctatcatta
     8281 attcaaagat tcgtaattaa aaatctagtt cttaaatacg aaccgaaacg gccgttagaa
     8341 ttaaagctaa aaacttaata agagtaatta aatatcatgt tttaggagga ttaactttat
     8401 gataatggat tttattaaaa aaattcctgc aggtatgatg atagtgccaa tgtttatagc
     8461 agctttcatt aatacctttt gcccagatat tgtacaaata ggttcgttta cgacagcagt
     8521 attttcaagt gttgggtctg caactataat tggggttcaa ctggtatgct tgggaactca
     8581 gcttcaattt agagaaatgc caaaagtttt aaagagaggt ggtatacttc ttggttctaa
     8641 atttgctata ggtgctttga taggtatact tataggaaag ctatttggaa tggatggtgt
     8701 ccttggtctg acaacattgg ctgttataag tgcagttaca aatagtaatg gtagtatata
     8761 tctttcattg atgacttcat atggagatga aacagattgt gcaactatga gtttacttac
     8821 tttaaatgat ggaccatttt ttacattaat agctcttggg acttcaggtc ttgcaaatgt
     8881 accattgttg tcattattgg ctgcaatagt gccaatactt gttggtatga taataggaaa
     8941 ttttgacaaa aaaatgaaag agtttttaga gccagggtgt aatttactcg taccttttgt
     9001 agggtttgca cttggtagta gcataaatct tgcaagtata ctaaaaggag gtttatcagg
     9061 agtattactt ggattaatat ctgtatttgt aggaggtata tttatagtat tctgtgataa
     9121 atttatagga aaacgtccag gatatgcagg atgggctgta gctacaactg ctggaaatgc
     9181 aattgctgta cctgctgctg ttgcattagt tgacccatca tggcaaccat atgttgcaac
     9241 agctacaaca caagtggcag ccgcagtagt tgtcactgca atattagtac ctcttataac
     9301 tagttggtgg gctaagaagt atggatgtcc taagttccca aaacaaaatg aggatggtac
     9361 aataacagct taatttatat tacagtgagt tgttggtaaa ataataaatt ctttaattag
     9421 attttaggat attaataaaa ttatattatt tataaaggag agcaagaata atgataaatg
     9481 cagtgataat tgatgaaaaa gataatgttg cagtagctat tgaagcaata gctaaaggta
     9541 gtgaaattag ttttaaatta aaagacaaaa cagtaaaaac gattacagca ctagatgata
     9601 taactattta tcataaattg gctataaaag atatggccaa aggtgataaa gttactaaat
     9661 atggtgaaca tataggagaa gcaaatcagg aaataaaaag tggtcaacat gtacatattc
     9721 ataatgttag aagtgttaga gaggatttgg ataaacaagt tatgactaat atataaaata
     9781 atatttatag agttataaaa gttgtattca ttgcttttag tgtcaaatag tttttaaata
     9841 ggtttttagc tgtttgatta gtgagtgtaa taaaaattat aatatataaa aattataaaa
     9901 agtatataga ctattttagg aggaaatcag tatgaaattt tatggatata aaagaccaga
     9961 tggaagagtt ggtataagaa accacatatt aatcttgcct acgagtgtgt gtgcatcaga
    10021 tacaactaga ataatagcat cacaagttca aggggcagtt acctttaata atcagaatgg
    10081 atgttcacaa gtacattcag accaacaatt aactatggat gttatggctg gttatgcagc
    10141 aaatccaaat gtatatggtg taatagttgt atctttggga tgcgaaaatt gccaaaatga
    10201 tttggtagtt gatgcaataa aagagagaac aaataagcca ataaaaactc ttgtaataca
    10261 agaagaacat ggaactttaa aaactataga aaaagctgtt cgttatgcta gagaaatggc
    10321 acaagaagct tctttgctta gaaaagaaga atttccaata tctgaactta tactaggaac
    10381 tgaatgtggt gggtctgacc caacaagtgg attagcagca aatccattaa taggtgaatt
    10441 aagtgataaa cttgtcgatt taggagcaac atcaatttta agtgaaacta ctgaatttat
    10501 aggtgcagag catatactag ctaggagagc agtaaatgaa gaagtaaaag aaaaaatatt
    10561 acatatagta catagatatg aaaattcatt aaaattggtt ggagaagaag ttagagaagg
    10621 taatccatct cctggaaata tagctggtgg tttaacaagt ttagaagaaa aatcacttgg
    10681 ttgtatacat aaaggtggtc atagacaaat aagtgaagta tatgattatg ccaaacaaat
    10741 tgataagaaa ggccttgtta taatggatac ccctggaaat gatgcttcat ctgttgctgg
    10801 tatggtagca ggaggagcac aaatagtggt atttagtaca ggaagaggaa caccatcagg
    10861 aaatccaata gcaccagtaa taaaaataac tggaaataaa ataacttttg caaatatgga
    10921 agataatatg gattttgatg ctagtccagt tatatatggt ccacaaacaa tggaagaatt
    10981 aacagatgat ttgttaaata tggttgtaga tgtagcaaat ggaaaacaat caaaagcaga
    11041 gtcccttgga tatactgaaa tggcaatagc tagagtatgc aactatgtat aatctttaag
    11101 taagaaaata aatacataga attttaataa agggtgagat tatgaagttt gtaagtttta
    11161 ctgaaggaga aaatgaatgt aaaagaactg gagttttttc taaagatgaa ttatttatta
    11221 tagatgttaa ctctttaaat ctcagtagaa attttaagga tttaaatgaa ttaattcaaa
    11281 atgtatctag tgaagatatt attaagttga aaactattat aaatgaaagt acattctata
    11341 attatgatgt ttttaaaaga gagagatgta ctgtacatgt tcctatagaa aaaccaatac
    11401 atgatatctt atgtgtggga gtgaactata aagaccatct tgaagaaact caaactcact
    11461 ttgatgatag ttttgaagaa ccacaaaaaa cggtttattt tacaaaacgt gtgacaaagg
    11521 caataggtcc agaagatgag atagatggtc attttgagat aaataatcaa ctagactatg
    11581 aagttgaact tggagtagta ataggtaaaa caggtgttaa tattagaaaa gaagaggtag
    11641 aagattatat ctttggttac actgttataa atgatatttc tgctagaaag ttgcaaaaag
    11701 aacatgttca atggtttaga ggaaaaggat tagatacatt tacctcttta ggtccatgta
    11761 ttttacataa aagtgcagtt ccttttccta taaaacttaa agtatgtagt agggtaaatg
    11821 gtgaagaaag acaaagttct aatacaggat tattcatatc agacgtttca gacatagttt
    11881 cagaactttc aaaaggtatg accttagagg ctggagacat tatagctact ggaacacctt
    11941 ctggagtagg gatgggattt tcacctcctc gatatatgag tagtggagat gtagttgaat
    12001 gtgagattga gagtattgga attttaaaaa attacattaa gtaactgttt taatataatt
    12061 aatattaaag ctaaatctga tgtattaatg aacatattaa aataaatttt aggagtttaa
    12121 attatgaaag cagaattttt gacaccagtt gttacaatat ttgatgaaga aggaaattta
    12181 gataaagaag gtaataaaaa agtgtatgac catcttatag atggaggtgt agatggacta
    12241 gtcataatgg gaagtactgg agagttcttt aatatgtcta tggatatgca aaaagaactt
    12301 atagatttag taactagtca tgtaaataag agagtaaaag tgtttatagg tacaagtcgt
    12361 atgtgtataa atgaatctat agaactttca aactatgcac ataatagtgg agcagatggt
    12421 gttatgataa taagccctta ttatttctct cttgatgatg atagtattga gttattttat
    12481 agtgaaatag ctaagaatac agaggctgaa atatatttgt acaattatcc agatagaact
    12541 ggttatgatt taagtccaga acttacatta agacttgcaa gaaaacataa aaacatagtt
    12601 ggatataagg atacagtaac tttgatggga cataccagag atttaataaa tactatgaaa
    12661 atagagttcc cagaatttaa ggtttatagc ggttatgatg aaaactttat acataatcta
    12721 atgtctgggg gagcaggttg tataggggga ttatcaaatt taattccaga agtttgctcc
    12781 aaatgggctg aagcttacaa taatagagat atggagaagg ttatagaaat tcaaaaatat
    12841 attaataaag caatggattt ttacttaata accaatccat ttataccagc aatgaaaaaa
    12901 gctatgaata tcagagggtt acaaataagt gattatatga ctgctccatt tgtaaaacca
    12961 aatgaaaaac aagtatgtga aattaaaaaa ttgatgaatg aattaaatat atgttaaatc
    13021 aaccttgcaa agaggtcaac aataaattac aattatctta ataaaaatta aattaaaata
    13081 atactagggt actatattag tttaataggg aaagtgatga aaattcacta cagcccccgc
    13141 tactgtatac ggatacgaaa tcaaaatcca ctgaaattta taaaaatttt gggaagggtg
    13201 atggaggaaa agccgtgagt caggagacct gcccagtatt atataagttc aatttagggt
    13261 ggagattgta atttataata aaatgaagta tcaattcgtt tttgtaaagt tgatatatga
    13321 acttctcctt attaaattag gagaagtttt ttattttatt aaaaggttat acttattaaa
    13381 gatgacttta ggataacttt aataagtata ctttaaaaag catatattat taaagttttt
    13441 tagccactca taaaaaataa gatattggag tgaaagagat gaaatttaaa tcaaatgttt
    13501 taaagcagtt ttttttaatt gctatggtaa tttttatggt aggaggtctt atagggtgta
    13561 gtaaaagtga caaagagaat aaacaagcta gtactggtaa gaaattagaa ttaaaatatg
    13621 ctagtggatt tagtgttgaa gatttagaag atggaattaa aaaagtaact gatggagata
    13681 atcgagaact gattttaatc ccaaagaaga ttaaaattcc tgaaaaatat aaaaatgcta
    13741 atatagttag aacgcctgtg gacaatgtat taatagcatc tacaacacag gcttgttctc
    13801 tgagaacaat agatgaacta gatagtataa aagcagtaac aacagaagag cagtactgga
    13861 ctattaaaga aattaaagat ttaatgaaag atggaaaaat acattacgtt ggttcaaatt
    13921 tagcaccaga ttatgaaaaa atagtagact taaatcctga cttaactata gttagtagtg
    13981 gtttatcaga ggctgataca aaatttattg acaaattaaa agaattaggg cttaattatg
    14041 ttgtagacaa tgagtataaa gagcaaaatc catttggaag aatggagtgg acaagtttaa
    14101 tagctgcatt ttatgataaa gaagatatgg ctactagtga actaaataaa actgtacaaa
    14161 aggtagaaga agtaagtaaa aaaataaaaa acaaagctaa acctaaagtt gtatgggcat
    14221 ctgtatatga aggaagtgct tatatagctc ctaaagattc atatgtggat aatatgataa
    14281 aaatggctgg tggaataaat gcatgtgctt caatagattc aaatgaaggg cgagtaagta
    14341 ttgaacaatt gcatgaagtt gcaaaagatg cagatatatt tgtatactca tcaagttcag
    14401 attatgcacc aaatttagat tctataaaat ctagtgcacc tgtattggct gatttggatg
    14461 ttgtaaaaaa cggaaaccta tgggtgtttg ctccagatta ttatcaatca gtagataaga
    14521 cagacgaatt aatagtagat ttagttgaga tgttccaccc aggtactaca ggtgaaaaaa
    14581 tcaaacatta tataaattat gataaatagt acagagagga cacaaaaaga taggttttat
    14641 agattttagt taatatagct ttgctaagag gagtatacaa atggatttta tcaataaaat
    14701 gagaataagg aatacgacaa tatttatagt attaattata agtatgatac ttactttttt
    14761 agtgtgtgta gcatttggct ctgtaaaaat tgaatttaaa gaagtaatag atgttgtatt
    14821 aagaggtatg gataatacag aacattataa aataatatac aatataagaa tccctagagt
    14881 acttgcaacc atggtaggtg gagcatgcct agcagtatca ggtattttat tacagatatt
    14941 ttttaaaaac ccaatagttg aaccgtatat attaggagta tcttcgggtt ctactttatc
    15001 agtagctctc atgattttgg gaggatacac attgggattt gagagtgtaa attcaatgag
    15061 tatatttgta gcagctttta taggagcttt aattgtaatg tctatagtca ttttatttgc
    15121 aaataaggta aagaatataa caactctttt aataatagga attatgaccg gatatgtatg
    15181 tagtggtata acaaatgtgt tacaatcatt tgcaaatagt gataaattaa aagaatttac
    15241 tatgtggtct atgggaagtt tttctggatt tacatgggaa aaggtagaaa tacttataat
    15301 agttggtata ttaggtcttt tgatgactgt attcataata aaacctttaa atgctttttt
    15361 actaggagaa gagtatgcaa aaagtattgg cataaatata agatttttta gagttttgat
    15421 agtatttata tctagtatat tagcagcaat agtatcagca tttgcaggac ctatagggtt
    15481 tattggactt gcagtgccac aaatttcaaa aatgatattt aaaacatcag ataatagagt
    15541 tttgatacca gtagtaatat ttttaggcag tatagttaca gctctttgtg atttaatatc
    15601 aagagtaatt ttatcaccag tagaattacc aataagtacg ataacatctt tattaggagc
    15661 tccaattgta gtaatgttat tggtgaaaag gaaggatagg gcttagacat gttgaaaact
    15721 aataatttaa gtgtaggata caataacaaa gttgttataa gtaatataaa tgtagaagta
    15781 aaaaatggag aaatactatg tcttttggga agtaatggag ctggaaaaac taccctatta
    15841 aggtccttat ctaaacttat atcaccaata aaaggtgaaa tatatttgaa tggtgtaaac
    15901 ataaattgta tatcaagaaa agcattatca aaaaaaatgg ctttagtact tacaaataga
    15961 ttattaggtg atttaatgac tgtacaagat atagtcaata taggaaggta tccatataca
    16021 ggattttttg ggagtctatc taagaaagat ttaataatgg tagatgaagc attggagtct
    16081 gtagatgcct tacatttaaa gaaaagatat tttgatgaac taagtgatgg agaaaaacaa
    16141 aaggtgttgg ttgcaagggc tcttgttcaa gaaccagaaa taattatatt agatgagcct
    16201 acaactcact tggatataaa acatagattg gaactcataa acatactgaa aaaattaagt
    16261 aaagaaaagt caatatcagt aatactttca ttgcatgaaa tagacatagc attaaaaagt
    16321 tgtgataaag ttgctcttat aaaaaataat aaagtcatag cttatggtca gcctgaagat
    16381 gtagtagatg aggatattat aaattcgctt tatgaattgg atgataaaaa ctttaatagt
    16441 cttttaggtt cagtagaaat tagtaataaa tctaaaaatg aagtttttat aattggtggt
    16501 gggggtaaag caacaccaat ttatagagct tttactaaaa aaggtatagg tctgtattct
    16561 ggaataatac atgaaaatga tattgattat gaaattggta gaactatggg gataaagatg
    16621 tttacagaaa atccatttga gcctataagt gatgagagtt tcgatttggc tattagaaat
    16681 ttaaatgatt ccaagattat aatagataca ggatttagtg taggagagac aaataagagg
    16741 aatatagata ttataaaaga ggctttaaaa ttggataaaa aagtttattc ttttagaaat
    16801 agagatgaga gtaagaagta ttatgacagt ttggatagta agatagaaca tatagataaa
    16861 gtttctcaaa taatcaatag tgcagatata aataatttat tataatttat gaaataaaat
    16921 gtacttatgt gctatagaaa aaagagtata gattataatt tttttgaaat tacaatctat
    16981 actcttttgt tattttgttt atgtatttat atatttataa aactttagca ttttatagat
    17041 aaccaatagc ttcctctcta gttaaaaaaa agtgaatccc tgtagtcgat tctttccatc
    17101 tattttcatt aaaatcttca gctacaatcg tttctcctac tctatatata aaattttcat
    17161 cagcatatgc acgagcactt ttaaaagatt ctttaccatc tatactagta attgacagta
    17221 cttttgcctt atcacatcta caagcgtttg atgtagcaga cactcttttt gcgtcgctgg
    17281 gtattaataa ttgtaccatt ctaaaattaa aacatttttt atagcctata aatgctcctg
    17341 tttcaggaca atacaattta aaaaaacggg tactttcatc tgatattact ccatttaaag
    17401 tagcatgttc taaatttgaa ttttctaaat tagctcctct aatatctgag ttagataagt
    17461 cacaaaatct aaaacttgca ccacttaagt ctgcattttt taatatagaa ttttttaatg
    17521 aacaatccga aagataagaa ttattaaaaa tagagttttc taaatctgct ttttctaaat
    17581 ttaaattgat aaggtcagac cacgaaaagt caatattact taagcagtat cctcttaagt
    17641 ccatattttc taatcttaat tcttttaaat ctgccttttc tccagcatta ttgtttttaa
    17701 gccataaata atgtttctca agtgtttttt ctaattcttg tttagaaata gttttcatta
    17761 aaatcacctt cataaattta tttttatata tgtagtagca aaatagtcat atatttaatt
    17821 atataacaat aaataaaaaa cttctaaata atatataaaa tatcatcttg aaagtacttt
    17881 tttcaatata tcagaatata tttttaagct atttagagat aatttaaatt tttaaacagg
    17941 acataataag cttgtattgt tttatatttg agttgataaa aatttcttta aataaaaata
    18001 atgtcaatta taaagtaata aa
//
LOCUS       DAENCV010000075        17565 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAENCV010000075 DAENCV010000000
VERSION     DAENCV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.076"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            130..492
                     /locus_tag="KQD82_002523"
     CDS             130..492
                     /locus_tag="KQD82_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG4380986.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            928..1629
                     /locus_tag="KQD82_002524"
     CDS             928..1629
                     /locus_tag="KQD82_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG4380987.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1900..2718
                     /gene="larE"
                     /locus_tag="KQD82_002525"
     CDS             1900..2718
                     /gene="larE"
                     /locus_tag="KQD82_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG4380988.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2773..3921
                     /locus_tag="KQD82_002526"
     CDS             2773..3921
                     /locus_tag="KQD82_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG4380989.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4065..5453
                     /locus_tag="KQD82_002527"
     CDS             4065..5453
                     /locus_tag="KQD82_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4380990.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5482..6180
                     /locus_tag="KQD82_002528"
     CDS             5482..6180
                     /locus_tag="KQD82_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4380991.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6183..7616
                     /locus_tag="KQD82_002529"
     CDS             6183..7616
                     /locus_tag="KQD82_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4380992.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7638..8375
                     /gene="truA"
                     /locus_tag="KQD82_002530"
     CDS             7638..8375
                     /gene="truA"
                     /locus_tag="KQD82_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG4380993.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8486..8693
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8946..10145
                     /locus_tag="KQD82_002531"
     CDS             8946..10145
                     /locus_tag="KQD82_002531"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG4380994.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWNIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10392..11438
                     /locus_tag="KQD82_002532"
                     /pseudo
     CDS             10392..11438
                     /locus_tag="KQD82_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
     gene            11546..12709
                     /locus_tag="KQD82_002533"
     CDS             11546..12709
                     /locus_tag="KQD82_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4380995.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNHSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12798..13178)
                     /locus_tag="KQD82_002534"
     CDS             complement(12798..13178)
                     /locus_tag="KQD82_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG4380996.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13181..13840)
                     /locus_tag="KQD82_002535"
     CDS             complement(13181..13840)
                     /locus_tag="KQD82_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG4380997.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13946..14920)
                     /locus_tag="KQD82_002536"
     CDS             complement(13946..14920)
                     /locus_tag="KQD82_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG4380998.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14960..15307)
                     /locus_tag="KQD82_002537"
     CDS             complement(14960..15307)
                     /locus_tag="KQD82_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4380999.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15525..16481)
                     /locus_tag="KQD82_002538"
     CDS             complement(15525..16481)
                     /locus_tag="KQD82_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG4381000.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16924..17292
                     /locus_tag="KQD82_002539"
     CDS             16924..17292
                     /locus_tag="KQD82_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG4381001.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6953 a         1816 c         2827 g         5969 t
ORIGIN      
        1 ataaatgttt aataatttga aatatgataa ttttttatgt tgaaagctta taatgattat
       61 gatataaatt ggttatagta tacaatttat gggtataaca gtaaaaaatt agaaaggaat
      121 ttttataaaa tgattaaatt atatggatat acaaaatgct ctactgttaa aaaagctaaa
      181 aattggctta aggaaaataa tttagaattt gaagatatag atatggttca aaatcctcct
      241 agtaaagagg agttaaaatc aatttataag actagtggat atgatataaa gaaatttttt
      301 aatacaagtg gtatgaaata tagagaattg ggtcttaaag atattgtaaa aactgaatct
      361 gatgataaac ttttagaaat attggtaagt gatggtatgc ttattaaaag accattactt
      421 ttggatggta aaaatgtatt attgggcttt aaagaagatg tatggaaaag tactttgcta
      481 aaagaagatt agaaagagat aggtgtgttg attaaaatta ttaattaata cacctataaa
      541 tttatgtatt aaatctaatt tttataaata ttaattagca aattcaaatc aaaaaaattc
      601 atatcagcaa acatattact tcttattaca aattatatca tatgttttat attaaaatat
      661 tataatatta caacgaattg ttacaaaatt acttattatt aaccatgaac atttgttttg
      721 tgctatcata tttatataag gtaattatca taaaaaatat cttgttcata cagttttata
      781 tagaaaaaag aaatgaagct tttctttata taagtatatt ttgtaaattg cctctatatt
      841 tatagtttaa ataatagtgg tattaaaaat ctgacaacaa aaataaaact ttcaaaaaat
      901 taaatctaaa aagaatagga gataaatatg agtattgaag atttatatga ttttgaatgt
      961 gttcctttga acaatgagat tgatattgat gttttatttg aaaagatgat agaagatgga
     1021 aaagaaaaag aatatacacc aattatagtc attgatgaaa aagtagggct attaagaaaa
     1081 aatatagata tgattactaa agagtatggt tctcttgaaa actatagaga gtattgttta
     1141 acaaaatata atgaaattga tgtaaatcaa ttttttgatt ttaggaagag tgagatttca
     1201 gaattattaa aggagctaat tgatttagaa gatgattttt atgattataa acattttaga
     1261 ccaattatga atatagatat atttcatgaa tataacaaag tttacatagc taaaattcca
     1321 acaattaaac catatgaagt atttgcatac atacctattg gcggatttaa tgattgtcct
     1381 agagatgaag aacatatagc tattgcaaaa tattggtatg aaaagcatgg aatctttcca
     1441 atcgcaattg gatgtgatac agttcaatat tctgtaaaaa atttaactaa agatggtaaa
     1501 aaatttgatg acttatgtat tgaattagta ttttattgtg aggatattat tatacaaggg
     1561 tatgaaacct taaaggctct taaggatgta ttaaaacgct ctacaatagt gcttttttgg
     1621 tgggattgat attctatcat ttagatagta ttaaattgtt agtcaccaag aatccacttg
     1681 ctttagttat ggggagtgtg tcaaactagg tttttataca cagtgtagca tagtaatatg
     1741 tttaaattac aaaggttaaa aaaatacttt aatattttta atattataat taatagattt
     1801 aaaggtaatt ataatttata aaataaatac tttatatgtt ataatcatta agaatgtaaa
     1861 aaaataatat atagttttat agttcatatt tttccagata tggaggtaaa ttttgtgaag
     1921 gaaaaagaaa aattagataa attaaaaaaa atgttacttg aattaggcag tgttgtagtt
     1981 gcttattctg gtggagtaga tagtaatttt ttgcttaagg ttgcaaaaga tacattaggt
     2041 gaaaatgtta tagctgtgac tattcatgca atgatgcatt caagtaggga aattgaagag
     2101 gctaaacaat atactcaaaa ttttggtgtt aaacatatca tattaaatat agaaaacttt
     2161 gatttaaaag aatttaaaga aaatggtgtt gatagatgtt atcattgtaa aaaatatata
     2221 ttctctaaaa taaaagaggt agcaaaagaa cataatataa agtacatagt agatggaaca
     2281 aatatagatg atttaggaga ctatagacct gggcttaagg cattgagtga attaggagta
     2341 attagtccat taaaagatag tagtttaaaa aaagaagaaa ttaggtcgct ttcaaaaaca
     2401 ttaggattaa atacttttaa taaaccatca tttgcatgtc ttgcgagtag gataccttat
     2461 ggagttgaaa taacagatga aaatttaaga atagtagaaa agagtgaaga atatctatcc
     2521 aatttaggtt tttctcaatt tagagtgaga atgcatggag atatagctag aatagaagtt
     2581 ggacaggaag aactaggtaa attttttgaa aataacaatt ttaataaagt tgatactaaa
     2641 ttaaaaatat ttggctttaa atatgttaca ctagatatgt caggatataa aatgggaagt
     2701 atgaatttaa atgtgtagat tattttaaaa ataaataagt tttatagtta atataaggaa
     2761 acaggaggtt ttatgaaaaa ttatacgtta atatcgccat gtttttttgg aatggaaaaa
     2821 atgctggcta gagagattac taatttagga tatgaaataa taaaaacaga agatggaaga
     2881 ataacatata aaactgatga gtttggaata gcaaagtcaa acatgtggtt gagatgtgct
     2941 gaaagagtac atcttaaaat tgctgaattt gaggcaaaaa gctttgatga attatttgaa
     3001 aatacaaaaa gaataaactg gtctagatat ataccttatg gagctcaatt tcctatatca
     3061 aaagcttcct ctataaaatc aaaattatac agtacaccag atgtacaagc catagtaaaa
     3121 aaggctatag tagaaagctt aaagaaaagt tatttggagg atggtttgct taaagaagat
     3181 aaggagaaat accctatttt tgtatttata cacaaagaca aagtaaccat ttctatagat
     3241 acaacaggag atgctttgca taagagagga tatagagaaa aggcaaataa agctccaata
     3301 agagagactt tagctgctgg gcttatttat ttaactccct ggaaagcagg tagagtttta
     3361 gtagacccta tgtgtggttc tggtactata ttgattgaag ctgctatgat tggtataaat
     3421 atggcacctg gcttaaatag agagtttata tcagaaaagt ggagaactct tgataagaaa
     3481 atatggtggg atgtaagaaa agatgctttt aataagatag ataatgaatc taaatttaag
     3541 atttatggat atgatataga tgaagaatct atagatatag ctagagaaaa tgctgaaata
     3601 gcaggtgtag atgaatatat agagtttaat gtaggagatg caactcaatt taaaagtgaa
     3661 gatgagtttg gatttataat cactaatcct ccttacggag aaagattaga agataaagac
     3721 agtgttaaac agttgtataa ggagcttggt tatgcattta gaaaattaaa aaattggtcg
     3781 tattatttga taacttctta tgaagacttt gaatatgagt ttgggcaaaa agcagataaa
     3841 aaaagaaaat tgtataatgg tatgttaaaa actaactttt tccaatatcc gggtcctaag
     3901 cctcctagaa ataataaata agttttacat gatttatttg taaaatataa aattttatta
     3961 aaataagtaa aacattatga tgccttgaaa cgtctaatta ttgtgaggag atttttgtga
     4021 acaaatttaa agatttaaag atttatagga ttaggaggag ttaaatgaaa gtgttaaagc
     4081 ttattactgt agtagccctg atgatgttat ttgttactgg atgtaatata aaaactgaat
     4141 ctccagaaca attagcgaag atacctgact atgatgatac aaaaaaagta ttgtatgatg
     4201 gtattgacca gttactaaaa ccagattcat ctatgatatt gccttctaat acaaaagaag
     4261 taggaaaaat aaataaggta gatttaaaca gcgatggaat tgatgaatta gtagtatttg
     4321 aacaaaaaga agatttaagt aataatgtga gtcaggtagg gtttgtaacc ttgagttaca
     4381 atggagaaaa atacgtactt ggagaccatt ttttagaaaa tggcgaatca atagaatatg
     4441 ctaattttta tgatttggac tcagatggat ataaggaagt tatcttattg gttaaatcca
     4501 aagacaaaac gaatatgcat atatataaag tagtagataa tgaaataaca aaaatatatg
     4561 acttggatgc atcatggctt caaaataaag aagattttaa tgacatgaaa gtaaaaattg
     4621 gatatataga tggagatgac aagttagaca tattgatgct tcatttgaat aataagacta
     4681 atgaaatgtt tgcaagtgtt gcaaattttg atgggaaaat gaaaatcaaa gattctgtta
     4741 agtttgaaaa tgtaaaaaac ttaagtgaat tatatataac attaggtaat gttgcttcat
     4801 ctgttggaaa ttcttctgca gctataaaag gtattgtact tgatattcct atattgaaag
     4861 ataataacta tattacgcag atactttata tgaaagatgg gaaaattaac aaagctttta
     4921 gtgattatga taaaactatt actaaatctt attatatacc tgtagatgat attgacaaag
     4981 ataagattat agaaatacct atagttgcag gcagtacagg taataataaa aatacttata
     5041 gttctaaatc atctgcaact ataagttggt atagatggaa tggaaaagaa ggtgctgact
     5101 caagtttaat atttacaagt cagatatatt ataattataa atataatttt aaattattta
     5161 ttccaaataa tttatttgat aaaatacttg ttgaacagga atttgttgga gaaaaagcag
     5221 tatttaaatt ttactatttt gactattcag accgtaagaa tttatttaat atagtagtag
     5281 agagtaaaaa taaacttgaa gatagaaaaa atacaggtac tcaaaattct attgtacttc
     5341 aagagagcga tgaatatact ttcttactag ttgtaaacaa tgcaaatgaa atggataagc
     5401 tagatatgac tatagacgca ttaaaagaat atttctcatt aatatatggt taatattata
     5461 ctcaattgga ggttttattt tatgaaggaa aagatactta tactagaaga tgaaattggt
     5521 attagaagtt ttgttagtat aaacttaaaa agggaaggct atgaaatagt tgaggcaggt
     5581 actggaagag aagctataga aaaaatgaca acagaaaagg atattacaat agctctttta
     5641 gatgtaatgc ttccagatat aagtggtata gaagtatgta aatttataag ggagaatttt
     5701 gaccaagtag gtataataat gcttactgct aaagctcaag aagatgataa aatagaagga
     5761 tttatctcag gagcagatga ttacatcata aaaccattta gtataaaaga gcttttagtc
     5821 agagtttctg ctcttcttag aagagtagca aaggatgatt caagtgttaa gtcgagtgag
     5881 atagtttctc caccatttat acttgatata gacaaaagaa aattatttaa aaatggaaag
     5941 gaaattgagc ttacaccaac tgaattttca atagtgaaat atctaatatc aaatgctaag
     6001 cagtcgttaa gtagagacca gatattagac gaagtatggg gaactaacta tttatatgat
     6061 tttaaaatag tagatgtaaa tataagaagg ataagaaata aaatagaaga tgacccatca
     6121 aaaccaaaat atatacaaac tatttggggt tatggatact gttttagaaa ggaagaataa
     6181 tagtgttaca ttttgaaggt ctgagaaaaa aagtaataaa aaactatttt ataataatta
     6241 ttataatggt tactctgttt gaaggattgt tcatgtttta catacaaaac tactattatg
     6301 attctgtcaa acagctactt gaatctgaaa taaaatatgc tgatgaatat aatgctatta
     6361 caatggaaac tacaagtttt gagaaaaaag taaaaaatat atttgataaa caacccttaa
     6421 ctaaaaattc agaatttgga atttcaataa tagataaaga taaaaatatt atattagacc
     6481 aatatggttt taaaagtaaa gaaaaagcaa actatgaaga tgttaacaat gcattaaaag
     6541 atataaaaac taaaaattta acaccttata cttatagaat accagataca ggagagcatg
     6601 ttatgagtat ctccttacca cttaaggtaa ataatataat agaaggtgtt gttagatata
     6661 cagtatcatt agatgctatt gataatgcaa tattaaagca agctacatgg cttatattag
     6721 caggaatatt tatacttatt atagctatac taataagtct taaatttgct gagactctta
     6781 taaaaccatt acgagagcta aagaaatttg caaatgaatt ggctgtagga aattataata
     6841 taaaattaga aaagatgaag attgtagatg atgaaattgg tgatttagca cagacatttg
     6901 aacacatggc acatgagatt gacaaaagtg aaaaattaaa agaggaattt atatcatctg
     6961 tatctcatga actaaggact cctcttacat ctataaaggg gtggagtgaa accttaggtt
     7021 atgaaagtat aacaagagaa gaactagact taggtttagg cattatacaa gatgaaacag
     7081 aaagacttat aaaactagtt gaagaattat tagacttttc aaggctttct tcagacagga
     7141 taaaacttca tgttgatata gttgatgttg aaggtttaat tgtaggtgtt gtaaatcaac
     7201 ttaaagttaa agcagctgaa aaagatatat cacttttatt tgaatttgaa aatgaattta
     7261 tagagaacat tcaaggagat aaaaatagat taagacaggt tctcataaat ttaatacaaa
     7321 attcatttaa atttacgagt caaggtggtt acataaaagt tgtagcttct caagatgaag
     7381 agataacaac catatcagta gaggataatg gttcaggtat agaaaagcaa aatttaaata
     7441 aagtgttaga taaatttttc caagaagatt ataataaggc tggtagtgga ttaggactag
     7501 caattagtaa tgaaattgta aaacttcatg gtggaagaat gaaaatagaa agtgaaaaga
     7561 atgttggaac aaaaataact tttaatataa aaaataaatt tgctaaacaa gcataaattc
     7621 ttagaaagag gattaagatg agaaacataa aaatgatagt agcatacgat ggttcaagat
     7681 ataaaggata tcaaaaatta ggtgacaata atatgactat tcaagaaaaa cttgaaaatg
     7741 ttttaagtaa aatgactaat gaaacagtgg agataatagg ttctggaaga actgatatgg
     7801 gagctcatgc aagaggacaa gtggtaaatt ttagaactaa ttgtatggat tctttggata
     7861 aaattcaaaa atatttatat gaatatttac cagaagatat agttgtaaaa actgttgaag
     7921 aagttgatga aagatttcat tcaagataca atgtaaagtc aaagacttat atgtataaaa
     7981 ttgacaataa taagtatcat aatcctttta taagaaaata tgctactcat gtaagtaaaa
     8041 aattagattt agatagaatg agaaaagcaa gtgagtattt agtaggagaa catgatttta
     8101 ctagttttgc ttcttctaaa tctaagaaaa aatcaaatgt aagagaaatt tattctataa
     8161 atataaaaga agatgataat gttatagaaa tatatgtaga aggaaatgga tttttgtata
     8221 acatggtaag gataatagta ggtgctttga tagatgttgg tctaaaaaga agagctccac
     8281 aagatattaa gtatatgctt gaatcaaaag atagatgtca atcatcagat actgcaccag
     8341 ctaaaggact atgtctttgg aaagtaacat attaatcaat tatataaaac tctctttaaa
     8401 ataaaaataa aataaattat atagttgaca taaatggaaa aataaactat tatatatttt
     8461 ataagagaga tataaaactt tatatttaaa tcctgtgaga aagagagtaa cttctatgtg
     8521 ttttatagag agctgaggat ggtggaagct tggcaaataa gtattagtga aaagaacttt
     8581 ggaggatact aattgaattt agtatgttag taggtaatct cgccttaaga gaaagagtgg
     8641 ataagtttat cttatcaatt agagtggtaa cgcggatata tttcgtctct tggtttttcc
     8701 aagaggcgtt ttttaatata tttaattatt agtgtttatc tggtatgttg aacatttact
     8761 aattgattag ctaagtttat caataagagt ggtaccgcgg atatttcgcc tcttagtatt
     8821 ttaaaatact aaggggtttt tttagtatta aaatgatttt atgtgagtct aaattctcag
     8881 agtcattcaa tattaagaat ttctataaaa taaaaattat aatttaagat tgggaggaat
     8941 taaaaatgaa ggaaaaagta gtattagcat attcaggagg acttgacaca tcaatcataa
     9001 taccttggtt aaaggaaaat tatgaagata tagatgttat agctgtttgt ggaaatgtag
     9061 gtcaggaaga taaaatggaa gatgtatatg aaaaagcttt acaaagtgga gcatcaaagg
     9121 catatgtgga tgatataagt gaagaatttg taactgaaac tatatttaaa gccgtaaaag
     9181 cagaggctaa atatgaagga aaatacttat taggaacttc tttagcaaga cctataatag
     9241 ctaaaaaatt agttgaagtt gctcataaag aaggtgcaaa atatatatgc catggatgta
     9301 caggaaaagg taatgaccaa gttagatttg aagctacaat tgcagctctt gacccgacta
     9361 taaaagttat tgcaccttgg agaatttgga atataaaatc aagagaagat gctatagact
     9421 atgctgaaaa acataatata aaggtaacag caactaaagc taaaatatat tcagtagatg
     9481 ctaacctttg gcatgtttca acagaaggtg gagatataga gcacctagaa aatgaacata
     9541 aaaaagatgt ttataaacaa tgtgtagacc cagaagatgc atgtgatgta gctgaatatg
     9601 tagaagttta ctttgaaaaa ggtgtaccta aaaaggtaaa tggcgaagaa ttatctccag
     9661 ttgcactgat tcataagtta aatgagttgg gttgtaaaca tggaattggt gttatagata
     9721 tagttgagaa tagacttgtt ggtatgaaat caagaggtat atatgagact ccaggaggaa
     9781 caatccttta tgaagcacat aatattttag aaagtgctac tttagacaaa gatacattac
     9841 atttcaaaca aatggtatct tataaatacg gagaacttat ttacaatgga ctatggtatt
     9901 gtaaattaag agaatctata gatgcattta tggaacaaac tcaagataat gttacaggaa
     9961 cagtaaaagt taaactttac aaaggaaata taaaacctgc tggaatattt actgaaaatg
    10021 ctctttatga tgaaggaata tcttcttttg gaaatagtga actttatgac cacaaagatg
    10081 cagaaggatt tattaattta ttcactttac cattaaaaat cagagctatg aaagctggaa
    10141 aataataaaa taaattattt aagagtgtat aaaaactatt tttaggtttt tatatgctct
    10201 ttttttattt gaatttatta aattatacag taaatagtat aaaaaaatat attatagttc
    10261 caaatggctt aataatgtga aaatataaga cctaaaatat aaattttata aatacaatag
    10321 ttattgactc tctcattgta agagggttta tactgaatct aatgaaaatt tatagtgagg
    10381 agatagatta tatgagaaaa ataattgaaa ttaaacatca ttcacatgat tatgagtgta
    10441 tgtggaatgg tatagaagat ttatatataa ataaaaccgg tgaatgcctt ccaaacgatt
    10501 ttttcttttt actgtcaggt tttggttcat tttgttatat gaaaacaaat aaagctgatt
    10561 tgaaaagaat ggtagctttg ggagatggaa ggacaaaaca gatgtataaa tttttagcac
    10621 caattgttgg atttgaatac aaacatcatg agtttaaaaa gtatgagcaa gcattaaaga
    10681 aagcaaaatc tgaaattgat gatggatatc cagttatttt aggagcttta gatatgtatt
    10741 atctaccata ttataagaag ctatatcata aagaacacat tccttttcat tatatattaa
    10801 tggtaggata tgatgatgaa ggaatatatc tgtatgattg tggtagaaca gaattattac
    10861 atctttctta tgataaatta cgtgaaagca tgaattgaaa ttcccctgga ttaagcaaag
    10921 aaaatactat ttgcacaata agaatgaata cttcaaagaa taaatataac attgctagag
    10981 aagctattgc tataaagcgt gatatgtttt taaatccacc taaaggattt ttgggatata
    11041 aaggttttga aaagtttatt aaagagttgc cacagtggaa cactgaattg gataaagatg
    11101 attatgataa gattttgacc aatatggtta tgttttttgg aacagtacca acaattccaa
    11161 atgcaatcaa aggaattaaa gaaccagata ctgttcaatt taaaggtgga tttgataaaa
    11221 tgagtagaat gctcaacagt attggggagg aatacaaaaa cgactctttt atacaagtag
    11281 ctgatatttt tgataaaact gctattataa ttgagaaaat atccaatatt ataatagatt
    11341 acttaactca aacatgtgat gatacaggac aacttccgtt attgttctct gaggtcttag
    11401 agcatatgaa aacaggatat ttaatcttgg atgtataaaa aatatattgt tgaccctaac
    11461 attacgtaat agtgtaatat gaaaatatag cttgaatata ttagtaggta ttgatggctg
    11521 taaaattaaa atttgaggtg tggatatgag aacagtgaaa caagtttctg atttaactgg
    11581 aataagtgtg agggcattac attattatga tgaaataggt cttttaaaac caaataaaat
    11641 tacagatgca ggatatagac tttatgatga tgaatctatt aaaactcttc aacaaatttt
    11701 attttttaag gagattgata ttcctttaag agaggttaag gaaataatgt caagccaata
    11761 ttttgataag gttgaagctc taaaaaatca aaaaaaatta cttattttaa aacgaaagag
    11821 actggatgaa ttgatagagc ttataaatca aactctaaga ggagaaggaa atattgattt
    11881 taaagagttt gatatgagcg agtattttga tgttttagaa gaatttaaaa gagagcatag
    11941 gaataaggta attaaaattt atggtagtgt agaaaaatac aatgaatata tagaaagagt
    12001 taagtctaat gaagagaaaa ttgctaaaat ggctataaaa caatatggaa ctattgagaa
    12061 gtttgcccaa gctataaaaa caaattttag cagtgatata ttgaatttag gagaaaaatt
    12121 tgatagatat aaaaatgatt gtttaaaaga aaaacatcca aaattaaaag aattatatag
    12181 aaagcttgta gaagatttaa gtaggaatca ttcttcaaca gaccttcaag aaatagcaaa
    12241 agagataaca gatatatcta aaaaagatta tgaaattttc tctatggata ctggtgatga
    12301 caactggtat tatatggttc aaaattatct agtaaatcct atgtggatag aagaagtaga
    12361 taaaaagtat ggaagtggag caggtaagtt tattggacaa gtactaaaga cttatctaag
    12421 agatagaaaa ccaaagataa atacattgta tgaaaaactt gtagaagatt tgagtagaga
    12481 ttgttcttca agggaagttc aaagcattgt tgaagaaata gacaatgaaa tgaaaagaag
    12541 taatgaattt tataaaatag acaatggtga aagatacttt gattatatgt cagagcttta
    12601 tttacaagat tctaactata taaaagtaac tgataagaat tatggtgatg gtgcatctaa
    12661 gtttattgga gaagctttta agatttattt tgataataat aattgttgaa aatttggaag
    12721 taaaaaatat gtaagtttta taaaagcaca aaaataagga tgtagattag ctccatctat
    12781 atccttattt aaagttatta aaatatatag tttacatcaa acttaacact ttcaataata
    12841 tttcccaaat atgtttttat actttcaact tcttctaaag attttaattt attttcagta
    12901 taaattgctt tgtccagaat attaaacctt aatcctattt catcaaagta ctcatgattt
    12961 aaaactttgt accaatggtt tttttcttta ttccaactcg tagatattga atcagcttct
    13021 ttttgagctt tttcccaatc ttcattttct atagattttt ctaaaacaga tatattgtct
    13081 ttatatcctt ctgtaaaatc atatagttta ttgttaacgt aaaatccaaa taatacaaat
    13141 aagatagtcc atagtataac aaatgttgta gatttcaata ttaatcaccc tttttatttt
    13201 gaataaatat tttgtcattt tcatctaaaa cacaaataag aacatcttta aaagtttcta
    13261 tgtgattaga ttttaaaata cccattaacc agttagtatc tttttgagct atgtctagat
    13321 ttttatttat tattctacca tccagtataa gtgggatagg taaatgatta aaagctcttg
    13381 gaggagtact attgtagctt gatgctggta caacacttag attaccatca gtctccaaaa
    13441 tagcatattg tacatctttt aggttaaagt atccttgaat cctaagttgt tctaataatt
    13501 catctatact aattctttct tttttaagct ctttatatac aatttttcct ttatctatta
    13561 atacagaagg tttacctgat aaaaatgaac ttaattttct gctttttaat gataagaatg
    13621 aaattagagt ctgcatgatt acaagtccag atattgctgc aacaccattt atgattggta
    13681 tattattatt ttccattggg acagatgcaa cttctgcaat taatagtgtt ataactaaat
    13741 caaaacagtt catttcagct atttcacctt ttcccatgac tctaagtgca attagcactg
    13801 ttatgtataa tataatactt cttattaata ctactgtcat atctatcaat tactccttta
    13861 aaaaatattt acacttagtt agtatatcct atataaaaaa tttaattcaa agtataaaaa
    13921 accttaaata attggattca actatttaag gtttttttga taattttaat attaaatttt
    13981 ttatggattt tctgttactt ttatttattt taataacaga ttcaacaaaa ctctttttat
    14041 ctcctaattt taagataaaa tcaggatatt cttcaaattc attgagtttt attaatttga
    14101 aagaagaaga tagattttta aagttaataa ttttagagtt attcactaat acacttcttt
    14161 ttttaaaata aatttctttt ttacttatat ttagtatagt taatatttgt ttaagatata
    14221 ttattatatc ttctggagtt ctatcaaatt tgagttcaaa attttcatct tcaaagtagg
    14281 gaatataata taaaggtgtt ttgagagcag gtaatttatt atataattct tcaagaatat
    14341 cgatattttt aggggagtta aagaaaaagc atttacacac attcattaaa tccacagcat
    14401 ctgaaagtga gttatgttta atatctcctt ctacagaaaa aatttttttc atattcagta
    14461 aagatatttg cttagataag attctattat tgtatgtaat atgttgagat ataaaaggtt
    14521 gaatgtcaat catcatatta aataactcta agcatttacc tgtatattta tatactctaa
    14581 atgaagttag aaggccagtt atatcaaaat caccccaatt atatattgta acatcatcaa
    14641 atttacaaaa ccatttataa aaatcatcca taacatataa aagattagga gcattattga
    14701 tatctagttg gttcaatttg gttaaatttt tacatttatt tgaaagtatt ttattgtatt
    14761 ttggcttaac taaagagtaa taattatcta ttgtattaaa ttcgttatca actaaaactc
    14821 caccaataga aattaattcc gcaccattat attcttttgg tttattttta ttatcatcta
    14881 gtatattaaa ttcaaagtct ataaataact tgtatttcat aaatctaaag cacatcctca
    14941 ggtaaattat ttattgagcc taagcatcat aagaatcaat agatataatg actctaaaca
    15001 tcaaaggttc attacaactt ttatttattt cataatttaa ttcaaataaa tcttttaaga
    15061 tataatttat aagtattctt tcccaaccca tttttaaatt aactttagag tctattaata
    15121 tattttctat ctttttaaat atttctatat ttgctggtat tatactgagc tcaatattac
    15181 ttttaaacgg gttagactgt aaatctgtta taagtatatt ttttaattcc tctgtaagtt
    15241 cagaaattaa actatcacct atgtattttt ttatgatttc ctctctaatt tcttttaaat
    15301 ttagcaatat tagtacctgc tacttacata gtagcttttc ctttcctttt aatactatta
    15361 ttcatttttg aatcttattt agatatattt taagattaaa agtctttagc tatataattt
    15421 tttacccatt tttaacttat attaacatat tacaggctta tatctccacc ctgcttacat
    15481 tgaggatgga gtatattgcc tattttgata aaaatgatag gttgttattt gcctattcct
    15541 ttttctgata atttttctaa catatctttg gtcattttat ctaaatcata actaaactta
    15601 aagttccatt cttttactgc actactactg tctagagagt taggccaaga atcagctata
    15661 ctttgtctta cagggtctac atcatattct atcacaaaat caggaatata tttttttata
    15721 gaatcagcta tttcttcagg agaaaagctc atagcagtaa tattaaaagc atttctatgg
    15781 atgagtttat cagctggagc ttcgattaaa tccactattg attgaagtgc atcaggcata
    15841 tacatcatat ccatttttgt accttctgct atataagatt tatatctctt attttttaat
    15901 gcctcataat atatatcaac tgcataatct gtagttcctc caccaggagg cgtaacataa
    15961 gaaataagac ctggaaatct aacacctcta gtgtctactc caaattttga gtgataataa
    16021 tcacatagta attctccaga aactttagtc acaccataca tagtattagg tctttgtaat
    16081 gtatcttgag gagtcatatt ttttggagaa ttttcaccaa aagcagcaat tgaactaggt
    16141 gtaaaaaatt tacaatccaa ttctttggct atttcaagac catttattaa tccattcata
    16201 tttaagttcc aagcttctag aggtttactt tcagcaactg cagaaagtaa agatgctaga
    16261 tgaattatag tatctacatc atattttttt gcaatttcaa aaaaacgatt cttatctgta
    16321 acatctaata tctcaaatat accagattca catactggat taccttcttt tactctcctt
    16381 gaactagcga ttacattttg ttctccatac tcatttctaa gctttattgt taattctgaa
    16441 cctatttgtc ctaatgcacc tgttataagt atttttttca atttcacaac ctcctaaggg
    16501 tttaatttat gtagtactac ttatttttat tttagcaaat cttttgttta aatacaaaga
    16561 attaaagata ataatatacg acttaattga atgaagtgca gtaagtgcct aaaaatttaa
    16621 agctaatata taatggtcta ttgtataaca ataaaagagt agattaaatt attatatata
    16681 gattgtgtga ctaagtcaca gaaaatagag ttaatttaat atataataaa agcatatcaa
    16741 gtaaagcttt aaattaaagt aactttatat aaaaagtaat ataaattgat aaaattataa
    16801 atttatttat agtgaatata ggttggattt tatagataca aaaatagtat atatgatatg
    16861 tagaccatta gcatgtttaa agtagcaaaa catataaaat aaaatttgaa aggaagtata
    16921 aaaatgacaa gaccatcaat atatcatagc caaggataca cttgtgcaga ggcattaata
    16981 aagtcttata atgaggaaca caacacagat ataccaatct caataggaag tggtatgggg
    17041 gttggaatga atgttggaag tgtttgtgga gctgtaaatg ctgctgttgt tataataggg
    17101 tatttaaatg ggcgaaatag taatttagat gaaaatatag caaggaagta ttcaagagag
    17161 ctattgaata gagtaagaaa taaatacagc tcagaaatat gtgtatctct taaaaaaaat
    17221 aaagtaactt gtgaagaaat aataaatttc acatatgatg ctctaaatga tatgctaaaa
    17281 aatcaaaaat aattctaaga aacaagtatt ttacattcga tagaatactt gtttttttat
    17341 gatattttta ataagtatta gtttattata atttttaaat caattttatt atattttaat
    17401 tgaaacaatc tactttgaaa attactaact gacaaaagaa tatgattata gtatagaaaa
    17461 aatgtaaatt atttaatatt aagtgagagt atatgtttac ctatataaaa tcgtataaat
    17521 taatggctaa atttataaat tataaagaaa tatataggaa aacaa
//
LOCUS       DAENCV010000076        17307 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAENCV010000076 DAENCV010000000
VERSION     DAENCV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.077"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            103..849
                     /locus_tag="KQD82_002540"
     CDS             103..849
                     /locus_tag="KQD82_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG4381002.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            910..1584
                     /locus_tag="KQD82_002541"
     CDS             910..1584
                     /locus_tag="KQD82_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG4381003.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTHGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLGHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1952..3298
                     /locus_tag="KQD82_002542"
     CDS             1952..3298
                     /locus_tag="KQD82_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG4381004.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3325..4725
                     /locus_tag="KQD82_002543"
     CDS             3325..4725
                     /locus_tag="KQD82_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG4381005.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSNINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4935..6371
                     /locus_tag="KQD82_002544"
     CDS             4935..6371
                     /locus_tag="KQD82_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG4381006.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPAKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            6715..7659
                     /locus_tag="KQD82_002545"
     CDS             6715..7659
                     /locus_tag="KQD82_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG4381007.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDNNKQISIFD"
     gene            7828..8760
                     /locus_tag="KQD82_002546"
     CDS             7828..8760
                     /locus_tag="KQD82_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG4381008.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGNFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8865..9332
                     /locus_tag="KQD82_002547"
     CDS             8865..9332
                     /locus_tag="KQD82_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG4381009.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9576..10430)
                     /locus_tag="KQD82_002548"
     CDS             complement(9576..10430)
                     /locus_tag="KQD82_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG4381010.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10596..12560
                     /locus_tag="KQD82_002549"
     CDS             10596..12560
                     /locus_tag="KQD82_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4381011.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKFLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12560..13021
                     /locus_tag="KQD82_002550"
     CDS             12560..13021
                     /locus_tag="KQD82_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381012.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13024..13344
                     /locus_tag="KQD82_002551"
     CDS             13024..13344
                     /locus_tag="KQD82_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG4381013.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13360..14460
                     /locus_tag="KQD82_002552"
     CDS             13360..14460
                     /locus_tag="KQD82_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG4381014.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14580..17180
                     /locus_tag="KQD82_002553"
     CDS             14580..17180
                     /locus_tag="KQD82_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG4381015.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6876 a         1783 c         2992 g         5656 t
ORIGIN      
        1 taattagatt tgataaacaa aaaataaaaa tgttgattac attaataaac tttaatataa
       61 tatatttatg aaaaatgtaa actgtgaaat ggaggtagta acatgaaaat tgaaaatctt
      121 tctattgatg atattaaacg aggctatata tttaatgatg atttaaattg ttatagttgc
      181 attaaatgta caaaaacata tgaggtagga gaaatatata aaataggtaa tagattcttt
      241 gaagcttcaa aagctattga acttcatatg gaattagaac atagtgatta tttagagcaa
      301 ttgcttaata gtgattcaaa gtacaacaca ttgactgaaa atcaaaaaca attatttcaa
      361 ttatttatta gtgatttacc agataaggag atagcaaaac aggttggagt atccacatct
      421 actatacgcc atcaaaaatt tattttcaga gaaaaagcaa aacaggctaa gttctattta
      481 gcactatatg aatctgtatt tgaagataaa tctaataaaa gtagtactat aataccaatc
      541 catgaacatg ctcgaatggt agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac
      601 attttggata catcatttga aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa
      661 gaaaagaaaa aggtcgtaat tttatctaag attttggaac aatttgaacg tgggaagaaa
      721 tattcagaaa aggaagttaa tcagatttta gaatctatat atgatgattt tgcaacaatt
      781 agaagatatc taattgagta tggttttatg agtagaaata aagaatgtac aaagtattgg
      841 ttaacataat taaaataata aattttacta atgtgtaatg ttatttttat ctagggggag
      901 gagtcaaata tgaatgatga attaaaatct attttataca ataaaggtgt agacattgtt
      961 cgctttgttg atatatctga atttccaata aatcaaacgc acggtttttc taaagcaatt
     1021 ttattttgta tagggttatc aaaaaaattt attacagata tatacaataa cttacctaca
     1081 gatagtgatg agtttttgga aaaagaggag aaagtagaag aattagctga ctggatttca
     1141 aaatatattc aaaataaagg atatagagcg tattctcaat cggagaaaaa caatctagga
     1201 catggatatt ttgaaaaagc ctatattaat ccagaaatgc aatcagggat tagtccttta
     1261 ccacataaga caatagcaaa tattagtggc attggattta tgggaaaaaa taatttgttt
     1321 gtaacagagg aatatggatg tgcttttagc atgtgtacag tacttacaga tgctccaatt
     1381 tctgttgaaa gatatccttt gattgattca aaatgtatgg attgcaatgt ttgtgtagag
     1441 aactgtcctg caaaagctat acatggaaat gaatggactt tacctggaaa aagagaatct
     1501 attattgatg tttcaaagtg tttttgcgta ttgaggtgta tgatgagttg cccatggtca
     1561 ttaagatatg caaatcaaaa ataattttac tagagaaggt attaaattga atattataaa
     1621 atttttaaaa tgtaagatta tataagattt ttcaaaataa aaatttgtat agaacactca
     1681 gaaatgagtg tttttttatt ggaattaaat tatattgata caaagcttaa ctattttaat
     1741 cattatataa actttgtatt aaaaaattaa gaataacttg ctaatagtaa aaatataatg
     1801 agattataat tgatttataa tataactgta atgatgttgt aaaatgaaaa ttgtcatctc
     1861 aaaattccca aaaagagtaa aagtcacttc ggttatagtc aaaaaatttt ttcagtgaaa
     1921 ataatttaga tttaaaaggg aggaattaat catggaaatt gaaactaaaa ctccagattg
     1981 gattaatgta ctagaattaa acagtaaaat agatattact gggaaatatg atgtaagtaa
     2041 tatgatacaa gatattgtct ctgataagtc actagaaggt tccattatat attttccaaa
     2101 aggtaattat ttatttgaga aaggtattaa aattagtcaa caaataacac ttcaaggaga
     2161 ttcttattat ggaggagata atcaagtatc aaatttagat aaaaaacaat caatcatagg
     2221 aacaaccaat tttataactc gaggagtttc taatatgagt attattgcat tgacaggaac
     2281 aagtcaatgt atcaaaaata ttaattttta ttatgatagt catgatatag agaaaatacc
     2341 gcctaaaaat gtatctgcta ttacagaata tggagaaaca caaggactat ctcattttga
     2401 acacctgttt atttctggat tttcagggat aggaattgaa attccatact attcaactgg
     2461 taatgatatt actgttagtt catgtggtct tggtatgagg cttggagaga agtcaatgct
     2521 ttctagtagc aagatttacg aatgtaaaaa tggaatggag attactactg gtgttagctt
     2581 aaataatgtg agaatagaag aaataaaaga aataggtatt aataataaag gatttggttt
     2641 taacctgatt atgaatctta tggtagacca atgtggatat tgtggattta tgtttgaaaa
     2701 aatgtgctat agccagataa ctgccagaat tactagatgc ggtcagtatt ataaaagtgt
     2761 tgattataat gcttatgaga atatggaaga taggaaagaa gaagcttatt ccatttttta
     2821 tggagatatt ttagaaaatt gcaatattgt attgatgaat agtaatattg ataccttgga
     2881 aaatgattca cttgatgaac ataagatata tgtaatcaag gcaaatgaaa caaacaatgt
     2941 aatgctaaca tgttatgctg aagcagataa ctttatacaa tgtgcaaaag gtaacttgct
     3001 gttagaaaat ggcgtaaata cttataaatt ttataatggt gagatttgtt ctgtaggagg
     3061 tgttcaaatt tctgatatag atgaaaaaga tttaatcaaa ataatcgaga acactctgta
     3121 tatagatgat aaaggtaagt atttaatggt tcctagatta aaaaagaata ctgtaatata
     3181 ttcagtacta gattctacag aagaaataaa taaattacat ggtggaatat gggaacagat
     3241 tgatgtaaaa gtattagctg gggaatccat gtactattat aaaaagataa aagattaaat
     3301 tatcaaacta ggaggtagtg attaatggaa gagtctataa taattaaaga tataagtggc
     3361 tggattaatg tacttgatta tggaatttgt aatgatgaaa caaaagatgt aagttttaag
     3421 ttgcaagaga ttatggatgc tgtaccagaa gattctatta tatattttcc aaaaggaaaa
     3481 tacttatttg aaagtggtgt caagattaag aaacgattaa ccttatgtgg agattcttat
     3541 attttagcga atgttcctaa aaatactgct ggagtaacac agtttacttt taaaggaaag
     3601 aaaaatccta aaagtgaaaa tatagcaatc ataacagcaa aaagtgtaag acactgtatt
     3661 aagaacattt gcttttattc agatagttgt gatagagata tgacatctaa tcatgaacct
     3721 ccaacagatg gaaaacctag atttcatcat aatgtaatta taaattatag aaatatatct
     3781 gctgttatat gtgaagataa acagggagaa ccaggtcatt atgaaaatct atcttttagt
     3841 ggtttttctg gaattgcact tgatatgcct aataattcta cagcaaatga tattacaatt
     3901 tttacatccg gacttggaat tagtacaggg gaaaattcaa caattgctaa cagtaaggtt
     3961 tggggctgtg ttgatggaat gataatttct actggaactt ttataaatgg tatgagggta
     4021 gaagaaataa aaaaagttgg aattaaaaat attggtagag gtttaaattt tattacaaat
     4081 atcacaattg accaatgtgg atattgtgga gtttggtttg attctatatc caatgttcag
     4141 atatcaggaa atatcacaag gtgtgggcag tactattatg atgtaagcta tgataattat
     4201 ttaatgttgg ataatagagt taaagaagca tattctttat tctatggtaa tttattggaa
     4261 tcaagtaata tagttttatc aaataccaat gaagacaatt gggaagattt caaagaggat
     4321 aagcataagg tgtatcttat agaagcactt aaaaccaaga atgtactatt aacatgtaat
     4381 atagatgcta ctgactggat tgttaagtca gaaaaaggaa atttaacgta caataatatg
     4441 cgacaaactg ttatatttta cgatggaaaa ctaagtaata tcaatggaat tggtatatct
     4501 gatgaagatt atggggataa agtcaagata aaaaaaggaa atatatatat aaatagtaat
     4561 gataaggata ttaagatgca taaaccagag gtaggtgaca ttatgtcaac aattattaaa
     4621 gataaaagta ttctttctgc tcattatggg ggaacatggg agaaaattgg tgaaaaaata
     4681 gagtttggaa taactgtaca ctattataaa ttgatagaga aataagtaaa tacgtagtat
     4741 ttgaatagat gtattttatt ggagggctag gactatagtt ttaatgtaaa tggtttggta
     4801 atagcatgga ttgaataata taaaaataat ataaaaaatt aatttgatta tgtaaatttc
     4861 tatgaagtag taaaactgtg actttagtca taggaggttc aaactaagta aaagggaaag
     4921 gaatatataa tattatggaa ggaagaactg ggattaaaga tggaagatgg attaatgtag
     4981 tagaaagagg tattaaaaat gatggttcgt acaacgtagg tcctgctttg caggatatta
     5041 ttgatgaaat agaaacagat ttttgtgtct tatattttcc aaaaggtaaa tacctattta
     5101 caagtggtgt taaaattaat aaagaaattg tattgcaagg tgattctaat tcatctacag
     5161 gtactacaca atttataact cgtggagtag aaaatatgag tattattaca ttgacaggga
     5221 agaaacaatg tattaaaaat ataaatttct attctgatag ttgtgagata caagtaaata
     5281 aagaaccacc ggcaaaaggt aatcctaaat atcattatga gatgattatc aataagaatg
     5341 aaaagaatgt tgagatgaat aatgtatcag caatcctgta tgataacagt aagaatatga
     5401 ttaaaggtct aggtcattat gaaaatattt atataagtgg tttttcgggt acaggaatta
     5461 ggataccata ttactcaatt gtaaatgata ttacagtttc atcttgtggg ctgggtattg
     5521 atacaggaat agacacaatt atttctaata gtagaatatc taagtgtcaa aatggaatga
     5581 ggattaggac tggaactagc ataaataatg taagaattga aaaaatacag aaagttggtc
     5641 ttataagtag tcaagttaag gggtatgagg ggtatggaag ttataaaatt aataatatta
     5701 cgattgacca atgtgggtat tgtggctttt catttgattc aatgaaagat agttattttt
     5761 ctggtattat aagaaggtgt ggtcagtatt attataatac agattatgac acatatttaa
     5821 gtattaaaga tagagtagaa gaagcatatt ctttatttta tggaaattat tttacagaat
     5881 gtaatattga tttagttagt gataataagg ataactggga tgatggctta gaatataaac
     5941 ataaagttta tgtatttaaa acccttcaaa taccaaaatt aacattgaga tgcaatatag
     6001 atgcctatga ttatatcgtt aaatcggtac atggaggagg taatttaatg ttaaaaaata
     6061 actttaacaa ttatatattt attgattcat ctagtccaga agttattaat ggaattaatt
     6121 ttaattatca aaagaatgga aacctgttaa ggttaaataa tggtaaatta agttataaaa
     6181 atggaaagga agaaacagct atattcagac ctaattatgg tcttattgta gcatcaacag
     6241 attcagaatg tgaacaaata gaggctacct atggagaaaa atgggagaag cttggttctg
     6301 aagtaattgg aaaagaaacg gtatattatt ataaacatca tgaaccagaa aaaacaaacc
     6361 aaatacaata aaatttgtat tatataatga tataaagttt taatatatag tgtttacctt
     6421 taatgtaata ttgatatatt tgtatagtat taaatttgtt aaaaagtaaa attgtagggt
     6481 gcttgaaaat agcacctttt ttagtttcat tataaaatta gttttgaaag tttaggaata
     6541 atgtatattt atttttaaag aattataact ttattaagga tattttaaat gctagaaatg
     6601 aatcagtaaa tcatactgga attatcttaa atgttttgat taaattaagt atttagagct
     6661 ataattatat taaaaatata ttagaacata attatattac tatggaggtt aaatatgata
     6721 gtaagtgtga gtagaagaac tgatatacct gctttctata gtaagtggtt ttttaacaga
     6781 cttgaagaag gttttgttta tgtaattaat ccaatgaatc caaaacaggt tagtaagata
     6841 gagttaaatc cacatactgt tgattgtttt gtattttgga caaaggatgt aactccaatg
     6901 ttatatgatt taaataaatt aagagactat aaatattatt ttcactatac tataacatca
     6961 tatggaaaag aagtagagac atgtatttta gataaaagaa aggtaataga ttcatttaag
     7021 gaattgtcta gaaaaatagg caaagaaaga gtaattttaa gatacgaccc gattttctta
     7081 agtgaaaagt acacagtaga ttatcatatc aaagcatttg cgagtttatg taatcagctt
     7141 gatggattta cagaaaaatg tataataagt tttatcgatt tgtataagaa aacaaaattt
     7201 aatactaaag cattacatat aaagccaata gaaattacag aaatagaaac attatcaaaa
     7261 gagatttcta agattgctca taagcatggg ataatacttg aaatgtgttc tgaagaatat
     7321 aatttaagta aatttgggat tggaaaaagt aagtgtattg atgatagatt aatatcaaaa
     7381 attataggat gtgaagtaga ggtaaaaaaa gatagtacac aaagagatat ttgtggatgt
     7441 gttaaaagtg tcgatatagg acaatacaat acttgcagac attattgtct gtattgttat
     7501 gctaacttta attatgcaaa agtagaagaa aactgtaggc tttataagga agataataaa
     7561 ttactcatag atactttaag agatgatgca aaaataagca ttagagatat gaagactatt
     7621 aaagtagata ataataaaca aattagtata tttgattaaa ataaatatac taattacctt
     7681 aatatattaa gaatatagat acttttgata ataaaattaa acttaataga tattttctat
     7741 ataagaaaga tggtcataat atgtcaattt gaccatcttt ttgatataat taaaacacaa
     7801 ataatttagt aacaaaggag gcagaacttg aatttattaa aaaaatatcc tatacctatt
     7861 gcaagtttga ttttatcaat ttttacttta ggtaacttat tacaatctta tagtgaatca
     7921 ttacgtaatg taattggatt tataggattt attttgtatg caatttatat agtcaaaata
     7981 atattactta gaaagggtgt taaagagcaa ttagagaacc ctttaatatc aagtacattt
     8041 ccaactataa caatggcaac aatgatattt gctacatata taaaaccatt atctttagaa
     8101 ttaagtatta tgatttggag cataggaatt gtgggtcatc ttgtgttaat agtcttattt
     8161 agtaagaagt tcttagttaa attctcaata aaaacggtat ttccatcttg gtatatagta
     8221 tatgttggga tagttgcagc tagtgttacg gcaccagcat ttaatcaact tttgattggc
     8281 aaaattgctt tttggatagg atttgtatct tatatagttc ttataccagt aattttaaag
     8341 agagtatgga taatcaagga aatgcctgaa ccatctttac caagtatagc tatattagct
     8401 gcaccagggt cactattatt gtctggatat attaattctt tttcagataa aaataatgta
     8461 gtattatact tgttattcat attatcaatt gtattttata ttatagtttt gggatattta
     8521 ccaaaattat taagattgcc tttttatccg agttttgctg catttacatt ccctatggca
     8581 ataagtgcaa tgggtatgaa actaatgaat ggttatttaa ctaaatcagg taattttata
     8641 agttggattt catatgcttc taagttagaa gagataattg ctacagtgat tattttatat
     8701 gttttagttt tgtttattat atatcttagt aaatctgaaa ctacatctaa agctaaataa
     8761 gaattattaa atatgtgtat ttaataggaa tattgatata aatatatgaa gataagataa
     8821 accatagaaa cttaatttag cttaatttta ttggaggaaa acaaatgaaa acaaaagaag
     8881 aatatttaaa agaagttaga aacatatcag aagaatactt tagaaaagga gagttttttt
     8941 gctctgaagc agttctccag acaataaatg atgctttagg ccaaccttta agtccagaga
     9001 taactaagtt agcatcagga tttccgatag gacttggaaa ggcacaatgt ctttgtggag
     9061 caatatctgg gggagagatg gctttaggta tagtatatgg aagagttcat ggtgaagcta
     9121 tgaatccaaa gatgtttgag catgctaaag gtttgcatga ttatattaaa aaagagtatg
     9181 gtgcaacatg ttgtagagct attacaaaga aatgggatgg tgataacttt atgtctcctg
     9241 aaagaaaaga gcactgtatt aaaataacag gtcaagttac agagtgggtt gcagataagc
     9301 ttatagaaga tgaaaaactt aatatcaaat agtgtaatat tagttggtga atttataaag
     9361 ttaaatttac ttatagtata agatacttga tattctcgta ttgatagaaa aatttatagc
     9421 atatgtattt aatgaaatat acttatattt agagtgtggt ttaatttcat aaatacatat
     9481 gctattattt taagtatata agcaaattta tttaaataaa tagaaacagt taatttatat
     9541 gtacaaaact atatatgatt agtgtaaata aatacctatt ttataatact caacataaag
     9601 acaccagaca tgattagtat tataccaata aaaatagcaa gccattcttt tttgttcttc
     9661 ttttctttaa aaaatataat ccctcctaaa gtggctacta tcacacaact ctgagagata
     9721 gaaaaactta ttgtaactcc caatgtatta gtcgttatga acatacctaa atttgctatt
     9781 gaccacaata taccagtaat taaattgaat aaaacatttt ccctatatac aatctcttta
     9841 gaaaaatact tgacaataat agatgaagtt atcaacatac caacagattg aggtaatata
     9901 attctgtatc cttcaattaa aaatatttga ttagttacaa catataatga taaaaataga
     9961 gatgataata atacaactcc aacacatttt aacatatttt tatctgtaga ttttttagtt
    10021 tgataatttg tgagaagtat accaagtatt atacataata ttgataaaga accaatcagt
    10081 aacattttag aactggtcca ttcgttaaac aagattacag ctaataatgt cgtaaaagtt
    10141 aattgtgaac catttgaaat tggcattact ttagatacac tagcaatttg aattgcttta
    10201 aactgcatta attgtcccaa actccagaat atgccagaaa tcatacttat acaaaatgaa
    10261 aaaaatgtaa tttcaggatg tataaccaga gttattatag tggaaaatat aaacgcactt
    10321 atggatgtac ctaaaagctg ttcatgagga gaacttttct ttagatttgc aactatgggc
    10381 atcaatcccc atcccattac aggaagtaaa gcaataatta ttgtcagcat taaactattt
    10441 cctttcatat taaatttaga cagcttttaa tttatcatct aaaattaaag tgttcaatac
    10501 ataaaaatta ccgtatttaa tcaggaaata ttagtagttt tttagaaaaa cattttccat
    10561 taaaataaag acaaagggaa ggagtaaaat tgaaaatgtt aactgatatg attcaaaatc
    10621 gtgaaattaa aataataaag tttttattaa aagaaggtca tacaactgta aaagcaatag
    10681 cagatgattt agatgtttca gaaaaaacag tttcaaaatt tcttaaagaa gtagaagcag
    10741 tgcttaaaaa gataggtctt tctttaataa gaaaacctaa gataggtgtt tatattgatg
    10801 gagatttaaa aaaaatactt ccttacctag ataataaagg aagtcaaatt cctacaacca
    10861 gagaggagag agttatctat atatattcag tacttttaaa gagtaatgat tatattacta
    10921 tacaacaact ttctgatgaa ctatatataa gtcgtggaac tattgaaagg gatttagcag
    10981 aagttgaaaa actgctaaaa aacgaaggga tttccatata taagaaacct agtaagggaa
    11041 taaagttaaa tataagtgaa agagataaaa gacaacttac tgctaaattt atacataaat
    11101 tttggagtag aaactggtat attaagcaag agcaagaaag cttttatcaa gcatttgaaa
    11161 agatacaagc agatgtaaat ggtatttttt cggaagatga tttatcaata attattgata
    11221 ttcttaggaa tttcagcaga gaaagaaaat ttgagttctc tgattatgaa tttcagtcat
    11281 tagttattca tttggcaata gctgtagaga gaattagaaa aggcgaatat atagaagaaa
    11341 gtttgtatac tggtaatata gaaaataatc aaatgtcaaa taccaattat ttagcaaaac
    11401 aattagaaga aaaattttca atattattac caaaatctga aattggatat ataaatttac
    11461 atttagtagc gtctgaccaa catagagata ctaatgaact aaaaaatact tcaactaatc
    11521 cttgtgagac aagtacaaat aatatatcaa atcttcggga aattataaaa aatacattgt
    11581 atgagacagc ttatgacaag gatttaattg atggattaac ttcacatgta cattcatcaa
    11641 taaatagact gaaatatgga cttagtatta aaaatccata tgtaaataca atcaaacaaa
    11701 acttttcttt atcatttgag caagctttag atttaaagaa agtaattgaa tttatatatg
    11761 atattaggat aaatgatgat gaatgtgctt atatagcatt acattttgaa gcttttagag
    11821 aaagaatgaa agaacttcca caaaatatta gagtaattct agtatgcagt acaggattag
    11881 gttcatctag gttattgtct gcaagaataa aaaaatactt tccaatgata aagattgaaa
    11941 aagtactttc cgtacaagaa ttagtatcca gtaaaattga tgcagattta gtaataagta
    12001 ctatttattt agagttagaa acaattccaa ccatagtagt tagtcctatt ttaaatgaaa
    12061 gtgatataag aaccttggaa agaaatatac aaaatgtttc aagttgtact tctaagaagt
    12121 ataaatcttt cagaaattta atctttgaag aaaatatatt gttgaatatt gaagcttcaa
    12181 attatgaaga tgcaattaat catataacag ataacctaat aaaaaaaggt tttgcaaaag
    12241 aaggtatagc taaaagtgca attgagagag aaaatttatc ttacacttct ttccaatcaa
    12301 tagctacacc tcatgctaat cctagttata ttaaaaaatc taatatatct gttgcaacgt
    12361 taaaaaatcc tattacatgg ggaaatgaaa aggtggaagt tattttcttt atttcattag
    12421 aaaatgacaa aaacctagag cttgatgaaa tatatgaagt tttttatgac tttattgaca
    12481 ataaaaaaaa tataaatgac atattaagtg caacttccac aaaggaagtg tacgatttgc
    12541 tagtggagga gagtatatag tgaaaatatc agatgtattg aaaaaagagc aaattgtttt
    12601 taatcttgat acaaatacaa aggaagaaac aataaaaaaa ttaagtagta tatttgttga
    12661 aaatgggatt gtggacaatg aagaagaata tgttaagtct gttttggaaa gagaagaaca
    12721 ttcaactaca ggtattggta atggtattgc tattcctcat ggaaaaagta atgctgtcaa
    12781 aaaatcttct ataatatttg caaagactaa gcataagatt gaatgggatg ctttagatga
    12841 aaaaccagta gatagtattt tcttattggc tataagcaat attgataagg atagcaatca
    12901 tcttgtacta ttatctaaat tagcaactaa acttatggat gatgacaatg tagatgcact
    12961 aaaattagcc aattcagaac aagaaataat caatatattt agtgaagaag gagagatgta
    13021 gaaatgaaaa gaaaaattat agcagttaca gcatgtgcaa caggagttgc acatacatat
    13081 atggcagctc aagcgttaaa gaaagcttca aaaaagatgg gtcaccttat aaaagttgaa
    13141 acacaaggag ctacaggaat cgataatgag ttaactacaa aggatgcaca aattgcagaa
    13201 gtagtaatat ttgcagtaga tactaaagtc agaaatgctg aacgttttga gggaaaagaa
    13261 atactagaag taccagttaa tgcacctata aaggatgcag agaaagtaat aaatgatgca
    13321 ctaaagttaa ttgacactaa ataaatggag ggaaatataa tgttgaaaaa gatagtctct
    13381 gagttaaaaa agcatgtctt aactggaatc tcatatatga ttccattagt aattgcaggt
    13441 gctatgataa tggcgatatc tcgtgtaggt ggttctttct ataatatacc agatatatgg
    13501 gatgctaaat atgcagaaag tgctagtagt attgttagat tattacatga tttagatggg
    13561 tttggaggta ctgcattagg attaatgttt cctgtaattg ctgcatttat aggattttct
    13621 atagctgata aattagctat tgtaccaggt cttgttggag gtatgattgc taaagatata
    13681 ggtgcaggat ttttaggagc tttggcagtt ggtcttatag caggatatac atgtttgttt
    13741 attaaaaatc atgttaaatt accaaaatca gcagcttcaa tagtaccaat atttatagta
    13801 ccagtatttg ggacattaat cactgttgta ttaatcaatt atgtgatagg tatacctttt
    13861 gccacattaa atactggatt agaaaattgg ttaaatggat tatctggtac aaatcagata
    13921 ttaatggcag ctattatagg ggctatgatg gcagttgact taggtggacc agttaataaa
    13981 gcagctttaa caacatcact tgcattatta acaagtggaa tttatgcgcc taacactgct
    14041 gcaatggtag gaatagttat accaccacta ggattgggac ttgctactat actagcaaaa
    14101 caaaaatata ataaacagtt acgtgaagct ggaaaatcat ctcttataat gggtttaatt
    14161 ggagtaagtg aaggagcaat tccttttgca gtagaatctc cattaaaagt aataccttca
    14221 tgtgtaattg gaacagcaat tgcttcagct atggcagttg gtttaggttc agtaaatgca
    14281 actccaatta gtggatttta tggatggttt acagtagaga attggccaat gtatgtgtta
    14341 tcaatagcag ttggaacagt aattgtagct ggattagtta tagtattgcg tggaaatcca
    14401 gtgattgagg atgatgaatt tgaagatgat gattttgatg aagcagagtg ggaatcttaa
    14461 aatttaataa ctatttaaga cgtgttataa gctatattca atatgaatct tatactaaat
    14521 aaagtgttaa acattcagtt tagacttaaa tataaaagat aaagaacaga ggagaataaa
    14581 tgataaaagc acatattgtt aatcatacac actgggatag agaatggtat tttacttctg
    14641 gagatgcatt agtacttagt gaacaactat ttacagatgt aattgatgaa ttagaacgta
    14701 atccagatgt aagttttgta cttgatggac aactttctat tttggatgat tatgtacaat
    14761 tacacaaaga aaaaatagaa atcattaaaa agttaataaa agaagataga ctacatattg
    14821 gaccatggtt tactcaaact gatgcattct ttgctagagg agagtctatt ttaagaaact
    14881 taatgattgg aatttttgaa agtaaaaagt atggtaagta tatgaaaatt gggtatttgc
    14941 cagatacatt tggatttaat gcacagatgc ctatactatt aaagcatgta ggtcttgata
    15001 atatattctt ttggagaggt atacatcttg gtaaacaggt tcaatcacca tattttaagt
    15061 ggaaaagctt aaatggagaa agttatatat atgcagtaaa tatgccacat ggatatggaa
    15121 ctggtatgct tttagagcct tcattaaagt atgttaatgg acgcttagac ccagctattg
    15181 attttattaa atcatatact gatgtagatg aggtgttaat accttctggg aatgaccagt
    15241 taaatataat aggtgatttc aaaaataaaa ttaaagcgat taatgatatc ggaaagtatt
    15301 catatataac gagttcatat caagagtttt taaaatatat aaagtctata gaatcactag
    15361 aaagctatag aggtgaattt agagaacctg tattagcaag aatacataaa tcaattggtt
    15421 ctagtagaat ggatataaaa ttagcatgtg ataaactaga aaacaagctg attaaaagaa
    15481 tagagccatt attagtaatt gctaaaaaga gtaaaattga aataagcaat cagttactta
    15541 taaatacatg gaaaaaactt ttggaaggac aagcacacga tagtttagct ggatgtgtaa
    15601 ctgatactgt aacagatgat attcttcacc gtataagaga agcaaatgaa atatgtgaca
    15661 gtattgaaaa tactattgta aaaaagattt cagaagggct taaattatct aaaaatgata
    15721 ttttagtatt caatacagaa gctaagcgtt ttaatggata taaagagata caagttgtaa
    15781 gtgatagtaa aaatatttat tttaaagata atttagatgc tactataatt gaagaaactt
    15841 atgtaaaacc gagagaaaat gtattagaag aaacacctgc tggtaatata tttattgaag
    15901 aaccaggata ttatatatta aaagtaagaa taaatataca gcttcctgca cttggttata
    15961 aagttgttag ttttgaatta agtgataaag aaatggtgtc acttgataaa tctaatgaca
    16021 cttttatatc taatgataat tataagataa tatatgaaga tggaagctta aatttacaac
    16081 ttaaagatgg aacttacata tcaaactttt taacattaaa agatagtggt aatgcaggtg
    16141 atacctatga cttttctcca ctaaaaaatg atgaggatat aaaactttcg tttgataaag
    16201 tgtatacaga aaaagcttta ggatatgaaa aaatggtggt taaaggtact actttactac
    16261 cacttacatt agaagataga aaaaacaaat atcttaatgg gaaattaaat gtaaaagtaa
    16321 gtattgtctt atctaaagaa aacccattga tggatgtaaa actttgtgtt gataacacga
    16381 tatataatca tagactacgt gttcatataa aaactgatat aaaagataat aaaaacatag
    16441 catcattgcc atttggatat ataacaagag aaaatggagt tttagacaac tgggaagata
    16501 tttacagtga aatgcctata gatttagaac ctttggaaag taatattacg cttacaaacc
    16561 aaaatagaag ttgtactgta tttactagag gaataaaaga gtatcaacat attgaagatg
    16621 aaattgcatt gaccttactt gctactacag atgaactagg aaagcctgat ttattatata
    16681 gaccaggacg tgcttctgga gatacaacta aaaaaggtca tataagaatt aaaacagata
    16741 aagctcaaat gcttaaggaa ttagaatttt catttgcaat atatatggat tctaaatcat
    16801 ttgatgaatt ggaaattgcg aatttaaccc acaattatct aagagaatgt gtaaattatc
    16861 aattacagga ttacaatttc tttttatatc gtatagataa taagattcaa aaatcaattg
    16921 tcaataaaac tgtaagtaga gaattagaaa taatatcttt accagaaaac tatcttatat
    16981 cagcatgtta tccttcttat tacagtaaag ataaatttat aataagagtg gaaaatccga
    17041 gtagtaatag aatgatattg gatgagcaaa tatttaaaaa tagaaatggg aaaattgtaa
    17101 atgcaataga agatgtagaa gaagaacagg tatatgaaat agcacctttt gacgttattt
    17161 caatactatt ggatttataa gtgtaaaagt aagtttattg tttaaagtct gacagatatt
    17221 aatctgccag actttttaat ttgtataaat ttgggtaaat attaatgaat ggttttagta
    17281 tttaagtatg aatttagtat ttaatat
//
LOCUS       DAENCV010000077        17180 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAENCV010000077 DAENCV010000000
VERSION     DAENCV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.078"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(264..788)
                     /locus_tag="KQD82_002554"
     CDS             complement(264..788)
                     /locus_tag="KQD82_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG4381016.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(853..1605)
                     /locus_tag="KQD82_002555"
     CDS             complement(853..1605)
                     /locus_tag="KQD82_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG4381017.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1636..2934)
                     /locus_tag="KQD82_002556"
     CDS             complement(1636..2934)
                     /locus_tag="KQD82_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG4381018.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINVSQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2951..5215)
                     /locus_tag="KQD82_002557"
     CDS             complement(2951..5215)
                     /locus_tag="KQD82_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG4381019.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5470..6288)
                     /locus_tag="KQD82_002558"
     CDS             complement(5470..6288)
                     /locus_tag="KQD82_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG4381020.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6281..6979)
                     /gene="cbiQ"
                     /locus_tag="KQD82_002559"
     CDS             complement(6281..6979)
                     /gene="cbiQ"
                     /locus_tag="KQD82_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG4381021.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6982..7266)
                     /locus_tag="KQD82_002560"
     CDS             complement(6982..7266)
                     /locus_tag="KQD82_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4381022.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7256..8008)
                     /locus_tag="KQD82_002561"
     CDS             complement(7256..8008)
                     /locus_tag="KQD82_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG4381023.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8190..8382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8514..10286)
                     /locus_tag="KQD82_002562"
     CDS             complement(8514..10286)
                     /locus_tag="KQD82_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG4381024.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10300..11229)
                     /locus_tag="KQD82_002563"
     CDS             complement(10300..11229)
                     /locus_tag="KQD82_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381025.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11457..11645
                     /locus_tag="KQD82_002564"
     CDS             11457..11645
                     /locus_tag="KQD82_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381026.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11638..12339
                     /locus_tag="KQD82_002565"
     CDS             11638..12339
                     /locus_tag="KQD82_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381027.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12341..13264
                     /locus_tag="KQD82_002566"
     CDS             12341..13264
                     /locus_tag="KQD82_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381028.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13421..14344
                     /locus_tag="KQD82_002567"
     CDS             13421..14344
                     /locus_tag="KQD82_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381029.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14337..15074
                     /locus_tag="KQD82_002568"
     CDS             14337..15074
                     /locus_tag="KQD82_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381030.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15085..15858
                     /locus_tag="KQD82_002569"
     CDS             15085..15858
                     /locus_tag="KQD82_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381031.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16399..17007)
                     /locus_tag="KQD82_002570"
     CDS             complement(16399..17007)
                     /locus_tag="KQD82_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG4381032.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFAISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5549 a         2965 c         2156 g         6510 t
ORIGIN      
        1 taagtgtttt ccacatattt tatatattta tttaatacat tattatttaa aaataaatat
       61 ataaacaatc ataataaaat ttttttaata aaactttatt ttttagttaa aatcatacaa
      121 tatatagatt tccacaatac aaataaatta aaaagtgaaa tgtatttgtc taaaaacata
      181 ataacatttc actttatcct catttttaag taatatatat ttttaatttt tatttataac
      241 taaatctagt ttttatataa attttagttt ttttctacat ctgagtaatc tattccaaaa
      301 gtttctacag taacagattt cataacttgt ggagtttgtg gcttatcagc tgaatctgtt
      361 ctaactgatg ctatttcatc aactgtatcc ataccttcta tcactctacc aaaacctgca
      421 tactgaccat ctaagtgtgg tgagttttta tgcattatga aaaattgaga accagctgaa
      481 ttaggtgcca tagttctagc cattgatatg actcctcttt catgttttaa attgttagta
      541 aatccattac cactaaattc accttttatt gagtgacctg gaccacccat tcctgtacca
      601 tttgggcatc ctccttgtat cataaatcct ggtattactc tatgaaatat tattccattg
      661 taatatcctt catttactaa tgatacaaag tttttaactg tatttggagc tatatttgga
      721 tataactcaa ttttgatttc ttttccattt tccatttcta tagttactat aggattttta
      781 ttttccataa tttattacct tccttttcat tagttaaatt acataattac atttattata
      841 ttatactcta aattaaagtc tatcaagtat attttttcct atttcatatc cttcttgata
      901 aatagtttta caaaactcat catcaaagtt aagcgtgtct ttaggagcaa attgagtatt
      961 aggtctaact actattattt tatcttcatc atatactttt ttaatatctt ctggcaactc
     1021 ataattatat tttgtggata ttaaaataat tttatccaca ttatccccaa ttaatggttc
     1081 aattaatgca ctcctaatta aacctccatc taatttgaaa ccatcataaa ttccttcttg
     1141 cacatctttc acaaattgtt ctttaaatcc aagagttgcg tttggatttg atggtaacaa
     1201 cccactgtat gaaatgctga taatggcttc attacgaggc atttttgata cgtcaacaat
     1261 cttttctgat actactttat tatcaacttc aacataatta acataaaagt tcatttcttc
     1321 tatattacta tcatctagtt cccttaggtt gtctatagca ggagaattat ccacagtatt
     1381 atttactatt tttacatttc cattctggat atcttttata tttgtccaca ttttctccaa
     1441 gttatcaaca ttccctgtgt acaaataata tccatttatt gctcctattg atgtccctga
     1501 aatcgaataa aatttattta ttcctccatc atacaaggct ttaactactc ctgcttgata
     1561 agcccctttt gctcctccac cttctaaaca taatcctaat ttcatagtct catctccaaa
     1621 ttgtttatct tatatttata tttcaaaaaa cttactaaat gttttatata tactatagtg
     1681 gtcctcaaca tttcctaatt ctcttataga atgcattgct aaaattggac ttcccacatc
     1741 cacagatggt atatctatat gagtagaaga tattggacct atagtacttc ctcctctttc
     1801 atctgaccta ttaacaaatt cttgatactc aacattagct tctctacata tatttttata
     1861 aatagctata gaatatccat cactagtata tgcctgacta acatttatct ttataaccgg
     1921 accttttccc attattggtt tattagttgg gtcatgcttt tctggaatgt ttggatgaat
     1981 agcatgagct aaatctgatg atatcataaa agatgaataa atagactcaa agaatccctc
     2041 tctatcctta cccattgata tgcaaattct ctccaatata tttaacaaca tgtttgaatc
     2101 tgcaccttgt ttagtagagc taccaacttc ttcattgtca aatacagcca ttacatttac
     2161 accatgttga cctttagtat taacaagagc attcaaacta gcatgtacca ttgcaagatt
     2221 atcttgtctc ccaatagata taaattcatc attagcccct attaaacttc ctttttcaaa
     2281 ttcatataaa aataaatcaa aatctataat atcgtccata tttacatcta agttagatgc
     2341 tatttgcttt ataagaaaat tatctttttc taatgtatca tttaacatcc caactaaagg
     2401 taacatatct ttttgcttat tatatgaatg accatcattt atacttctat ttaaatgtat
     2461 tgctagattt ggtattatac aaattggctt tttaaagtct ataagccttt catttggttt
     2521 taatatacta tcacctttta aaactactct accagctatc ccaagaggtc tatccaacca
     2581 tgtacttaat attgctcctc catagcattc tgtatttaac ttcaaataag atttttcagc
     2641 ttctatttct gcatttggtt taatactaaa tgtaggagaa tctgaatgag agcctattat
     2701 tctaaatcca tctctttcta tatcttctga atttactaca aaagctacta tagctgataa
     2761 attcttagta acataatact ttccaccaac tcttagattc catctttgat tcaaaactaa
     2821 ttcttcaaat ccatttttat ctaagatttc cttagtactc tcgactgcat gaaaagacga
     2881 cggactatca tatatgaagt ctattaagtt tttagcaaag tttatactat ccattcctca
     2941 tttacctcca ttataaaata tttagtgttg tatattcttt atttattttt ttaattccct
     3001 gttggtcaaa agctatagta acatctgtac caattataga aaccactgtc ccaaccccaa
     3061 atttaggatg atgtacctta gaaccaagtt ttatatcatc tggattagtt tctttattag
     3121 aatcttttat tgtagcatta actttatctg caactttaga tttgcttata gtactcatat
     3181 actttttagt atatttatct aacatattat aacttgcttt agaatagctt agttcttttt
     3241 ttactttgta caatctttct atacattctt ctggcagttc ttccataaat cttgaagcta
     3301 ttgcaacatt tgttttacca tataatgttc tcttctctgt caatgacata tatagctctt
     3361 ctttagctct agttatacca acataacaaa gtcttctctc ttcttctatt tgtgaatcac
     3421 tcatagattt tatagctctt gatataggaa ataacccttc ttccatacct gttaaaaaca
     3481 caacagggaa ctctaaacct ttggatgtat gaattgtcat aagcgaaacc ctatcattgt
     3541 cttcttcatc atttgattca gatgtaagtg ctatacttgt taggaatgtc tctaagtctt
     3601 tattttcata agtatcatca ttactacttt caaactccaa agctatagaa ataaattctc
     3661 tcaaattatc aattctgtct tgagcttctt cacctttgcc tgttaaatct ttttcatttt
     3721 tatttcttat ctctacaagc tcgtccatat atcctgtagt atccaagact ttttctataa
     3781 gtttgctaac aggataaact tcttttatag ttctaagagt ccctataata tcaacaaatc
     3841 cacttatact tgcccttgcc ttagtagata tatctgaatt tgtttctata tcaattaata
     3901 ctgagtatat actttcctgt tttaagttag ctctatcttc aattttttct atagttctaa
     3961 gccctatacc tcttctagga acatttataa tcctttttat agatatatca tcttgtggat
     4021 tttgaataac tcttaaatac gccaacaaat cctttatttc ttttctctca tagaattttg
     4081 taccaccata tatattgtat ggaatttgac ttctatttaa agcgtcctct acaggtcttg
     4141 cctgtgcatt tgctctataa agtactgcaa aatccttata aggtctattt tcttttctag
     4201 ctatttgtgc aattgaatca gcaatgaaat cagcctcttc tatttcgcta ccagttaatt
     4261 gtatctttat aagttctcct tctttttttt cactccaaag cttttttctt tttctttcta
     4321 tattattaga tatgacttta ttagctgcat ctaatatcac ttgtgtagac ctataatttt
     4381 gttctaactt tacaacatgt acattgtcat aatctttctc aaattcaagt atgtttctta
     4441 tatctgcacc tctccatcca taaatacttt ggtcatcatc tccaacaaca caaatatttt
     4501 gatgttctct tgctaataat tttatcaatt catattgagc tttacttgta tcttggtact
     4561 catctaccat tatgtatcta aatctacttc tgtaatatgc taacacttca tcattagcct
     4621 tgaaaagttc tacagtttta agtattaaat catcaaagtc aagtgcacta tttcttttta
     4681 aacggtcttg atatagcgca taaatatctg caatcttaac cattctatta tctgccatat
     4741 tgatatcttt aaactgcttt ggagtataaa gtttgtcttt tgctcctgaa attgcactta
     4801 taactgcttt tggttcaaac actttatcac ttaaatttaa ttcttttaga cagtctttaa
     4861 ccaatgtaac ttggtctgct gaatcataaa ttacaaaact tctattgtaa ccaatcttat
     4921 ttatatcttt tcttagtata cgaacacagc aagaatggaa tgtacttatc cacatatcct
     4981 tagtttcagg acctatattt tgttcaactc tctctctcat ttcatttgca gctttgtttg
     5041 taaatgttat agccaatata tttggtgctt gtacaccttt atcttctata agatatgcta
     5101 ttcttgttgt taatacctta gttttacctg aaccagcacc agccagtata agtactggac
     5161 cttcagtctt ttctactgct tctctttgag caggatttaa tgtatctaaa ttcatatttc
     5221 tcctcctgtt aacagccata aatatatttt aatctaaaat atgaattact tcaaattata
     5281 attatgtatt atattaatag attatttcca atatattaca attaaataac atttttccat
     5341 ataatttatt tcattgttta ataaaaaaaa taaccttatg atttggggaa tcataaggca
     5401 cctttaaggg gtctaggtta agggttatta tatataaatc tattttttta aaataggttt
     5461 agggcttaat tatttatttg tattaagttc tctttccttc caatacttat acaaatcttc
     5521 ttctttctta aataagggta aattcatatt tctatgtact tttactaacc atggggattc
     5581 tagaccagct tgtataatat tattttcatt tataaacaca ttttctgcct ttccttcatc
     5641 aatcaaaatc cccttatcca atacatatat gtaatcacat atttcataca ttaaattcat
     5701 atcatgactg gatattacta tttttatatt atttttattt aatcccttta ttatatctac
     5761 aatagaccta gtacttactg ggtctaaacc agctgtaggt tcatcaagta atacaatttc
     5821 attttccatt gctataactg atgctattgc cactcttttt ttctgtccat agctaagaaa
     5881 atgtactggc ctgtctataa aatctattcc attaacagct actagagctt tgtttattct
     5941 ctctttaatt gttttttcat ccataccaat gtttctcatt gcaaaggcta tatcatcata
     6001 tacttttgaa taaaaaattt gtttttctgg gtcttggaac acaataccta cattttttct
     6061 aaggttgtat aaacttctct tatcatattt taacttttct tctttaaaaa gaatttctcc
     6121 agatgtaggc tttaatatac ccattaaatt cataaataat gttgattttc ctgaaccatt
     6181 tgaacctatt atacctataa catttccctt tgaaaaatcc atattaatgt ttaatagtgc
     6241 attggtattt ttttcatatt gatacgttaa attatttatt ttaaacatca ttatccccta
     6301 ctatgtgaaa tttaccatca tacaatttta tgtctaatga tatacacata tcatcatacc
     6361 ttgtcataag tcttttgtac agcatacttc caagaatacc taaggattta tatgaagttt
     6421 ttaaatttat atatccaaat cttagctctt gtgacttgta tatttctgaa aattcttcta
     6481 aaaatatgaa tataaaccta tatattaaca ttgataactc gacaaaagtg tcaggtaaat
     6541 gaagtttttt aaaaaagaat attaattgat taaaaggtgt tgtaagtata caaaaataca
     6601 cacaagtcaa acaagacatc gctctaaata acacatgaat agacatgtca atagacgcct
     6661 ttgatattcc tacattaaaa ctaaaaaatt caaaactata taataaatct gctttattaa
     6721 aagatatatt tacaagagtt attcctatac taagaaataa aaagtacatt ggtattctaa
     6781 gtaattttaa ataatttttt aaatctattc cagccacaca aacaaccaaa atactcatta
     6841 aaatcataat tccacttaaa ataaatatat tttgtattac catagaagca atcagaaaaa
     6901 ttactcctat ggctactttt ttgtttggat ttactttact caatctattt gtatatgcat
     6961 atttatcaat tataagcatg attaacttct ctttcctttt tgaagaccta atatgtatcc
     7021 tattacacca gcccctaaag ctgcttgtgc agagaataat aaactttcta tttctccact
     7081 tggtggctca tatggactgc taaaccatgg tttgtaattt ggatttatct tagtaatttc
     7141 actttcagct tgaccatctg ctcctccata ttcagcacct gagttgacaa gtaatgggaa
     7201 tataataaga gctactacta ataaaagtaa taatatgtta gtttttgttt ttgttttagc
     7261 actcattagc tttaactcct ttatcattat tttctagtat gcttttttct ccgttttctg
     7321 taattaaatt atatacaatt acagtaagta taccttctgc tattgctatt ggtatttgag
     7381 tcataaagaa aattcctaaa aatttaattg ctgaagccat taccccacct gatgggtctg
     7441 ggaaagccag tgctaattgt attgaagtta ttgtatatgt ggctaagtct cctattgctg
     7501 ctgcaagaaa tactggaata gcattatttc catctttttt ctttaaagcc ttaaatatta
     7561 ggaatgatac gattggccct attatcgcca ttgaaaaagc attagctcct aaagtagtta
     7621 ttcctccatg tgctaataac aatgcttgga atattaaaac tatagttcca agaacaaaca
     7681 ttacgcttgg tccaaacatt attgctccaa gacctacacc agtaggatgt gaacaacttc
     7741 ctgttactga aggtattttt agtgctgata atacaaacac aaatgctcca cataaagcta
     7801 ataatacttt ctttttaggg tcttgcttta ctattttacc tattgatttt aatccgaata
     7861 agaaaaatgg tataaatata actccccatg ctatagacca ttttactggt aagtaacctt
     7921 ccattatatg cattgcattt gatgtaactg gagttaaaat gattaacata agtgcagcta
     7981 taacccctaa tttaatattc tgtttcatct tagtatccct ctctcccttt ctaataatta
     8041 aaacataata atacatgtct gatttattta ataaaataaa aaagcctaac tcgatggtta
     8101 ggcttctaat tttccacaat ctaaatacaa acacataaaa caaacatatc tgtataatta
     8161 ttataagaaa ctttctccat ccccgaagaa attactttaa tattttaggc aggtctcctg
     8221 acttagcttc atcctactcc tcttccttcc catgagatat aaaaaatatc atcatagtgg
     8281 ctaacaaaag tttcgtctgc atcacagtag cgggggctgt aaaggaattt aacctttttc
     8341 cctattaatc ttgcttaaag aacctataat attatatatt taatttttaa attattctta
     8401 aatcattttc tatataaatc attgtatacc ttttctgtga aaatttctac attttttata
     8461 aattttattc atttttatat ttttatgtat ataaattctt ttttgtaagt ttattaattt
     8521 ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat atctcttgaa
     8581 tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc ttttcctgta
     8641 aatactccta atactaatat attttgcttt ctagcttttc tcacttcatt ggctgtatct
     8701 ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc actacttttt
     8761 cctatcttta catcatttgg tcttccatcg ctgagaacta tcaggatttt attttcttca
     8821 tctctattta ttagaccact acatatagat tttattgcca aaccatctct attattacca
     8881 gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata atctttaaat
     8941 ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt tggtatgcca
     9001 acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc ctgatttcta
     9061 ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt ttcattttct
     9121 atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc tctatttgct
     9181 aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat taaaattctt
     9241 gacagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg ttctctaaat
     9301 tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac attattacac
     9361 tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac atttctacaa
     9421 ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc ataataatgt
     9481 tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac tcggtctgct
     9541 gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat ggatgaagat
     9601 attgaatcta tatcactttc aataatattt tcttcttcat cactataaag ttcttcatac
     9661 atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt ttcatcaata
     9721 agactatttt cttctccatt taatatgtat tcaaaataag ttaaatatat aacatctaat
     9781 gcttttaaaa tctccataat atcttgattg gtatctatat ttcttatatc attaactatt
     9841 cttctagtca tactatctaa tgctgggtgc ttgtccatac tataaagtac aaatgtgtat
     9901 ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac aatatcttta
     9961 taagcatttc ttcttatgtc ttctatccct ggcctatctt taatgacctt ttcttcaact
    10021 attgtattta atatgacttg aattagaccc agtataatat ctttgttgta tccttttctt
    10081 attctatttg taagatattt tttaactata tcccaatcta catactttct tctagcacct
    10141 gcaattattg cgaaatatag acctatttct tttgaagagt aatctttttc tgatatatct
    10201 atactttcat tgtaatcacc agctattgtc catgataggt tattagctct attttcaaat
    10261 tcataatcat catatatcca tttcataatc tcacctatat taaatatgac ttattggaaa
    10321 cacatctata ctctcccatt tattaggtat tctagtttta attacatctc caaccatttc
    10381 tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa gagttggttt
    10441 taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa tagctttact
    10501 agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg caaagtgtat
    10561 taatttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta acttatcttc
    10621 ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat taagttcttt
    10681 tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat gtagattgac
    10741 tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat gtaccacaac
    10801 tatagcatca ttttttgcca tattaatttc atcaaatacc ccaaaacctc cattaatagc
    10861 acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg tatctgtacc
    10921 tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag gtctattaaa
    10981 tatttcccct agattatcag caagtaagtt ttttcctgtt gcctttggtc ccgataataa
    11041 tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt taccataaaa
    11101 ataagtttta gactttgtta ctctgtattc taaatctttt tctaatccat aataatttct
    11161 aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta gattatctat
    11221 tatatccata tttttacccc ttttatataa atagtatttg tttcatttct tatcaccatt
    11281 ttagcataaa aggatataag ctaaaattga ttttttaatc tccccatttt aagtaaaacc
    11341 atgcaccatt ttatcactac aaaattaaga gtactactta ctgatatttc ttgattttgt
    11401 tcgtctatac gtatataata tatgctgata aattatgagg aaaggatgat tgaaatatgg
    11461 aatatatgtc ttgcccagaa gtagcaaaaa aatggggtat ttctgaaaga agagtacaaa
    11521 aactatgtga agaaagtcgc ataccaggag tttcaaaact cgggtatatg tggctgatac
    11581 caaagaacgc agaaaagcct gttgatggta gaacaaaaag aagaaaggaa gtacaatatg
    11641 aatagcgttt taattataga tgatgacaaa gaactttgtg ctcttatgaa aaaatgtgtt
    11701 gagcaagaaa atttatctgc tattactgca cattgtggtg cagaaggtct acatttggtc
    11761 gataaaaata gacacacact ctctcttgta attttggaca taatgatgcc agatatagac
    11821 ggttttcaag ttctgaaaaa aattagagaa acaagtaata ttccagtaat tatgcttact
    11881 gctaaaaatg acgaggatga taaagtttcc ggactacgtt taggtgctga cgattaccta
    11941 acaaaaccgt tcagcataaa cgaacttatg gctcgtgtca attctcttat tcgtcggtat
    12001 actatgttaa accctgcttt tataacagac acagattgca taataataaa tggcatgatt
    12061 attgacaaga caaaccgaat tgtttctgtc aataatattc ctgttcaact tacaagcaaa
    12121 gaatttgatt tacttgcttt tctggcttct aataagggac gggtgtttac caaaaaacag
    12181 atttatacac aagtatggga agaagaatat gcttttgacg atagtaacat catgtctttc
    12241 atcagtaaat tgagaaaaaa gattgaacca gaccccaacc atccatttta tattttgaca
    12301 gttcgtggtg tcggttatcg ttttaataag gaggcttaac atgaatacaa gtgtctatct
    12361 gcttttgata ttggttctta gcctgattat tatagcgtac cttgtttctg tcctgctacg
    12421 tgtccgaata caacttacac ttataaaaga tgctttagag gatataaaaa atggaaacct
    12481 taatcgacgt attttaacag atgaaaacga cattacaaaa caaatttgct atgatattaa
    12541 cgaaatagca atcaatagcc aatttcaact tattcagcaa aaacaatcag aacaagccta
    12601 caaacagcta atgacaagtc tctcccatga tgtaaaaact ccacttgctt ctttggtcgg
    12661 ttatttggaa gcagtagaaa gtaaaattgt tgtaggagag gaaaaagacg agtatatcca
    12721 tgttgcgtct aataaggctc attacttaaa gaattttgta gaaaatctat ttgaatgggt
    12781 aaaattggat tctaaggaac aagtttttca ttttgatatt tttgacttga atgaattatc
    12841 ccgtaatatt atatctgatt ggatacctgt tttagaaagc agtcattttg aatatgaatt
    12901 tgatatacca gaaatagagt acttcttacg tatagacgca aatgcatata cccgtatcat
    12961 taataaccta ttacaaaata ttattaccca tagtagtggc aacaaaatga cattgagaat
    13021 ttttgaaaac aaagagcaag ctcaaattat aataacagat aatgggaaag gtatatcttc
    13081 agacaatctc ccacatattt ttgaaaggtt gtaccaatgc gaccattcac gagcttcaaa
    13141 gggaaatggt ttaggcttgg cgattgcaaa ggaacttatt aacgctcata agggaaatat
    13201 taccgccaac agcactcccg gtatgggaac agaatttaca atattgcttc ctaaagccct
    13261 gtaaaagtac gaggctttct tactctaaaa caagataaaa gcaagattat ggcaaggttt
    13321 tggcaaggtt catgttttat aattctaatt gtagttgatt ttgacgttgt atcaactatc
    13381 ttttgtagaa acaaatttat taaaagtgag gaaattatat atgaatgatt ttgtgattga
    13441 aacaaagcaa cttacaaaaa tttatgggaa tcaaacagta gttgacaaag tcaatctcca
    13501 tgtaaaaaaa ggccgaatat atggtctgtt aggacgcaac ggagctggga aaaccacaat
    13561 tatgaaaatg attttaggat tgacttcaat cacgtatggc gaggtagatg tatttggaga
    13621 gaatattaag ggacgtgaaa aacaggtata tccccgtatc ggtgcaatta tcgaaacgcc
    13681 aggattttat cctaatctga caggaacgga gaacttggag atttttgcaa aactgcgtgg
    13741 tacagccgcc cctaattctg taaaaagagc attggaaatt gtaggcttac cttataaaaa
    13801 taaaaagctg tttagtaaat attcacttgg catgaaacag cgacttggta ttgccaacgc
    13861 tatcctgcat gacccagaac tgttagtttt agatgagcca accaatggtc ttgaccctat
    13921 tggtattgcg gagatgagaa agtttattag ggacttgagc gttgagcatg gaaagacaat
    13981 tcttatttcc agtcatattc tttcagaaat ctcattgttg gctgatgata ttggaattat
    14041 cgaccacggt gttcttttgg aagaaagtag tatgagtgaa ctagaaaaga aaaacagtaa
    14101 atatatcctg ctacaagttt cagatattcc gaagacatca cttattttag agcgtcaatt
    14161 taacacgaag gattattccg ttcaagatga ctacacgtta cacctttata acactaatct
    14221 ggatatgggt gaaatcaata aagcacttgt aatgcagaat gtaattgtta ttagttctca
    14281 actttgtaat gatactttag aggactactt taaaaaaatc acaggaggag agggcattgc
    14341 ttaaacttat tcaaacagaa tttcttaaac tgcgtcgtag gaaacttatt tggctgatgt
    14401 tgttgtctgc tctcattatg ccgtttttcg cactactata cttcaattat tttggtaaaa
    14461 caggtgttga gccaacaaag ttttataaat ggtctgcatt tggttataca ctttggatta
    14521 ttttgcccgt tgttttgggt atattttgca ccatgcttat acacgaagaa aaccagtatg
    14581 atatgctcaa acagctttgg attgtaccta ttagtaaaat gggatacttt tttagtaaat
    14641 ttttcgttgt tttgatttac tctatttgtt ttatgctgat tacagccgtt gcttctgtgt
    14701 tatttagtgt actttctgga tatgtcgtat ttagctgggc aagcatttta tatttgttga
    14761 aaaaatgtct agaaattggt gtaatcactg cttttgtaat gttgccgatt ttggcaatcg
    14821 ctacatctca aaaaggatac attctcccaa tttgtatcac attggtatat gccttttccg
    14881 gttttgttat aatgacaata aatatgtatt tacacccttt atccagtatg ggagttatta
    14941 tcatgagaaa taaagacatt ccaggtctta tattttcaca ggaaattaat gtcccattag
    15001 catttctttg tatttgtatt tggagtattg tatcagtatt gtttgcaaac attgcactag
    15061 gaagaagaaa gtgaggtgta gataatgtgg aaattacttt gggctgaatg tcaaaaaatt
    15121 cgtcgttcaa aaattgtttg gattacagtt tttgcatcca ttatgatagc agtcatagtt
    15181 tttgcaggag ggcaggatgt atataatgac ccagatttac attatggctt aaagagcgtt
    15241 tatgacggtt cacggtatat agacaatgca ggttggtata tggacgcagt acaaccttgg
    15301 tcaacttttt ttgttcttcc tgctgtcatt gccttattag gaagctatat tatttgtcgt
    15361 gaagaagagg aggacactat taaatccctg cgtcttattc ccataaatga aacaaattta
    15421 accatagcaa aaatgattat tacttttata ttcagtatac ttctttattt gttactcttt
    15481 actataacat ttttgactga atccgtttta catttttccg atttatcgac aaaactggtt
    15541 ttaagttgca tgaaagagta ttttcttgat ggcataggtg tattctttgc aatttctccc
    15601 attattgcct tggtatcgcg catgaaaaaa ggatattggc ttgcattagt attcacagaa
    15661 gtttattcta ttgctggact atttgcgggt atgtcaaatg tcttacaaac cttttttcct
    15721 attgcggcaa tatttaatat ttctggatac tatataacta ctggagaaaa aattatgggg
    15781 agtatcataa gtttattact ttgtgcctgc ctttctgcat ttatactgaa agggctaaaa
    15841 cacaatgaaa aaaattgaaa tatgtatatc aaaatgaaaa agataaaaaa actgattatt
    15901 ctaattgtat tcttgtttct ttttggcatg ttacactgtt atactgaatg gaaattcaac
    15961 aatgacccac ctaaaggggt aatagaaaga ataaatgagt ggactgataa gattggactg
    16021 gattagtaac ttatgttaat tatagtgaat gtaatattag caagttattg tttcgtattt
    16081 taatactata actttaaata tttggtttta atcttccaaa cgaatataaa aaaactgtca
    16141 cagaggcaac atatttttga atatttataa agtggtagtt ttgattttca aagctgccgc
    16201 cttttcttct atataagcaa acttatcctc aacaaagtgt aaaacatggt acattttaag
    16261 aaaaaagtac cacagacaca agcaataaaa gaaacgccga gaacctacaa aaacgtagtg
    16321 cttatgcaga tttacgacgc cttctatttt ctcaaccgtc ttaatgacct tgtacaaaag
    16381 aaatttttct aatatttttt ataatcttat cttgtgatgt tttttatgta taaatgactt
    16441 aataacaaat ccaataaaaa ttatggatat tggtataatt agaacgagga atttataatc
    16501 attataatgt gacattattc ttacccaact ctcagaaaaa atatatccta tactgattaa
    16561 tgaagtattc catatggtta ttcccacgat agatgatatt gcaaaagtta taatattttg
    16621 acctgctata cctgctataa aagatatgta tgttctacat aaagggatta atctacatac
    16681 acaaacagaa atagaagagt atttattaaa ataatcttga gatgcaaata ttcctttctt
    16741 tgcctttgga catatctcta ttattttatt tatgatagat ttacctcccc aatttcccac
    16801 tgtataacaa aatatcgaac caagtatacc tgcaattata cttaaaatta aaattgtcag
    16861 aaatccaaaa tgatttcttg cagcaattgc tccacataaa ggtaaaacaa cttcgctagg
    16921 taatggaaaa caagcatact caatcataat taatacaaat acagagatta atccatattg
    16981 taaaataaaa ctattgatta cagacactta cttatctatc tcctttctta tttaaaggtt
    17041 ttaagggtac atattctaag gattctcacc ctatatttaa agaatcaatc tctttaaact
    17101 attatatgta tttacacaaa gcatttataa catactttaa aaatagtacc ttaaaataag
    17161 aaacttaata aataaaaaaa
//
LOCUS       DAENCV010000078        17173 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAENCV010000078 DAENCV010000000
VERSION     DAENCV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.079"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            137..1042
                     /locus_tag="KQD82_002571"
     CDS             137..1042
                     /locus_tag="KQD82_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG4381033.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1166..2119
                     /gene="msrA"
                     /locus_tag="KQD82_002572"
     CDS             1166..2119
                     /gene="msrA"
                     /locus_tag="KQD82_002572"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG4381034.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2272..3402
                     /locus_tag="KQD82_002573"
     CDS             2272..3402
                     /locus_tag="KQD82_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381035.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3519..4487)
                     /locus_tag="KQD82_002574"
     CDS             complement(3519..4487)
                     /locus_tag="KQD82_002574"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG4381036.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4822..5010
                     /locus_tag="KQD82_002575"
     CDS             4822..5010
                     /locus_tag="KQD82_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381037.1"
                     /translation="MNYHTKEELVEALRVISSSIINCEKGQKKFSEETSHHTCFKNII
                     EAIYISKSLIMDEISKRD"
     gene            5270..6469
                     /locus_tag="KQD82_002576"
     CDS             5270..6469
                     /locus_tag="KQD82_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG4381038.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            6521..7024
                     /locus_tag="KQD82_002577"
     CDS             6521..7024
                     /locus_tag="KQD82_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381039.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            7131..7448
                     /locus_tag="KQD82_002578"
     CDS             7131..7448
                     /locus_tag="KQD82_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381040.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYSNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            7606..8565
                     /locus_tag="KQD82_002579"
     CDS             7606..8565
                     /locus_tag="KQD82_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG4381041.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            8680..9465
                     /locus_tag="KQD82_002580"
     CDS             8680..9465
                     /locus_tag="KQD82_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381042.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            9757..11076
                     /locus_tag="KQD82_002581"
     CDS             9757..11076
                     /locus_tag="KQD82_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4381043.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(11151..11579)
                     /locus_tag="KQD82_002582"
     CDS             complement(11151..11579)
                     /locus_tag="KQD82_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381044.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTSTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(11670..13046)
                     /gene="hydG"
                     /locus_tag="KQD82_002583"
     CDS             complement(11670..13046)
                     /gene="hydG"
                     /locus_tag="KQD82_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG4381045.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(13047..14132)
                     /gene="hydE"
                     /locus_tag="KQD82_002584"
     CDS             complement(13047..14132)
                     /gene="hydE"
                     /locus_tag="KQD82_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG4381046.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(14168..15376)
                     /gene="hydF"
                     /locus_tag="KQD82_002585"
     CDS             complement(14168..15376)
                     /gene="hydF"
                     /locus_tag="KQD82_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG4381047.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(15391..15642)
                     /locus_tag="KQD82_002586"
     CDS             complement(15391..15642)
                     /locus_tag="KQD82_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG4381048.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(16023..17063)
                     /locus_tag="KQD82_002587"
     CDS             complement(16023..17063)
                     /locus_tag="KQD82_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG4381049.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         6436 a         2054 c         2651 g         6032 t
ORIGIN      
        1 ttaaaggttt gaatgtaact taaatattaa aagaggaaca ttgtaatatg taacttgcat
       61 aaaatatata tactatagtg aaattatttt gattaaaatt taaaatataa ggtgttttat
      121 gtgggggtat atgtatatga aaaattctaa ttttaatata ttggcatttg atggtggtgg
      181 attaaaaggg gcattgagta tatccatact tgagaggata gtaaaagagt atccaaatct
      241 attaaacaat ataaatatgt ttggaggtac atctacaggg agtctaattg cactagggct
      301 tgcgtatggt gtaagcccta aagagataaa agaactttac tcaattgaaa attcaaagta
      361 tatttttaat aaaagttatg cagaaatact cagaccaaag tatgaaaaca aaaacttaaa
      421 agaagtttta ttaagtatat ttccagagga actagaatta aaagatttaa ataaattagt
      481 aatgatacca agtttttata taggaaatga ggaaaatgca tggaaaccag tattttataa
      541 caatatgcca aattctttta caaagactag caaagttgta gatgtagcaa tggcaagtag
      601 tgctgctccg gtattttttc caacatataa tcgtcatgtt gatggaggaa taatagcaac
      661 tgacccgagt ttagcgtgta taattcatgc cattgatagt ggatttagac ttaagaatac
      721 aagacttttt tcaataggga ctggatatgt atataacagt ataaaggcag atactaccga
      781 atggggagct attgactgga taattaataa agaacctgat ttaccaatta tatctattac
      841 cttagaagga aattctcaaa tgagtcagtt attttctcaa aaacttcttg gagataatta
      901 ctatagatta aatcctaaga tggaaaaaga tgtagctatg gatgattgtg atgcattaga
      961 atatctaatg tctttaggag aaaattgtga tataaaagat gcttttggat ggataaagaa
     1021 aaagagcttt tatagaaatt agattcaatt tgcaattgta tttttacaca tgatttcatt
     1081 gagtaggtaa aaataataaa atgcagatta ttaagttact tcttagggaa taaataaagt
     1141 ataatttatt taaggagtga tgaaaatgat aaaacttgct acttttgcag gtggatgttt
     1201 ttggtgtatg gtaaaacctt ttgataagta tgatggagtt attaaagtag tttctggata
     1261 tacaggtggt catgttgaaa accctacata tcaagaggta tgttcaggaa ctactggtca
     1321 tttagaagca attcaaataa catatgatga tgatataata agttatgatg aacttctaaa
     1381 tatcttctgg aaacagatta atcctacaga tgatggtgga caatttggtg ataggggaac
     1441 tcaatataag actgctatat tttatcatga tgaagaacaa aaaaatatag ctttagagtc
     1501 aagagaacat ctggaaaata gtaaaatatt tgatagtaaa atagtgactg atataattcc
     1561 agcaactaaa ttctacgaag cagaagaaaa tcatcaggat tattataaaa aaagaccatt
     1621 aaactacaat atgtattata aagggtctgg aagatataat tttgtaaaaa gtcattggga
     1681 tggaaacaat ataaataggg aagagttaaa aaagacttta actcctatac aatttgaagt
     1741 tactcaaaat gatatgacag aagtaccttt tgaaaatgaa tattatgata ataacaaaga
     1801 gggaatttat gttgatattg taagtggaga agtgttattt tcttcaaaag ataaatttga
     1861 ttctggatgt gggtggccaa gtttttcaaa gccagttata gataaatcta tcattgagag
     1921 aagtgatttc tcacaaggta tgtacagagt tgaagtaaga agtgctaaag gaaattctca
     1981 tttaggtcat gtatttgatg atggtccaaa agaacttgga ggtttaagat attgtataaa
     2041 ttctgcttct cttaaattta taccaagaga aaatatggaa gctttaggat atggagaata
     2101 tttaaaactt tttgagtaat taggaatatt tattataaac ttatctaaaa ggtgaaaata
     2161 taaaaaaata ttttcacctt ttgttttttt gtcaatagta tgataaatta ttaattgtaa
     2221 tcttatttat accaatagat taagaattta attttgaaag agagaaaaca aatgaattta
     2281 aaagacttta atggaaagag attaaaatca gctagaattt ttagagcaaa gacgatagaa
     2341 cagttgtcta aagaaactaa aataaataaa aaagatttaa aagcttttga ggaaaataag
     2401 tatgtaccaa atatagaaaa tacattaaaa ttatctaata tattaaattt tccaaaagag
     2461 tatttttata aaaatgaaaa tataaatatc gttgtagagg atagccactt taaccctcaa
     2521 tctaaactac ctagagtaga agagatttct tatagagaaa aaataattat aatacataaa
     2581 atatattcat ttatggagaa ttatattaag tttccaaaag aaaacttgcc aaatagaaaa
     2641 gtaatgtcag atattaatat aaatgatata gaatctcttg catataagac tagagagttt
     2701 tgggatttaa atagtactcc aatagtaaat atggtaagtt tgttagagtc taaagggatt
     2761 ataatatcag gtatgaatgt agatagaaaa ggggcaacta tctttactca aaaacagaga
     2821 attagtaaag aatctaaata tctaatatct ctgggtaatg acaaaaagtc agcatcaatt
     2881 agaaattata ctttggcatg tgagcttgga tatataatat caagtgaatt aagaatacca
     2941 agtaagcaat tttcagaaga tgagtatgcc tgtgcatttt tattgccaaa agagagtttt
     3001 ttaaaagatt tgactcatcc agaagattta gattattatg tagaactaaa gaaaaaatgg
     3061 attgtaccta tatctgctat gatttttaga gcatataatc tagaaaagat aaattacaaa
     3121 aaatacaatt atcttatgaa tgaaatggat aaaaaaggct ggttgataga agaaccacta
     3181 gacaaaatga aaggttcaag cccaacgtat ttaaaaagag cagttgaatt attgatagaa
     3241 aataaaataa tgagtgtaaa tagtatagta tcaagtttag aggaatttgg aataaatctt
     3301 tatccagaag acttagaatt attgatggga ttgaaaaaag gattgttatc tcaagaggta
     3361 aataaaaaaa gtaaagttat aaaatttgat ggtaaaaaat aggtttatat tttagtatta
     3421 aaattaaaag caggtacaaa tgatttaaac attaacttag ttaaaagtct tttcaataag
     3481 tcatttacct gcttttagtg taagaaagaa tattgaatct aaagtgtttt tgaaagtgca
     3541 atatattccc ttaaaacaga acaagattta ttaaattcgt tttgctcata ttcagtcata
     3601 ttgatttcaa taatttcttc aataccatct tttcctataa cacatggaac acctgcaaat
     3661 acatttcttt cgttatattg accttctagt aatgtagaaa ctggaagaac ttttttctca
     3721 ttgtgtagta tagcttttac aatttctact aaagcagtac ctataccaaa ttctgtacaa
     3781 tctttaccat ttattatact tattcctgtt tttttatttt tttcaactat tgatggtaag
     3841 tctaaattgc tataagtact tgggttgtct tttatcatgt ctaagattgg tttaccacca
     3901 attgaaacat gagaccaagg aaccatttga gaatctccat gttcacccat agaataagct
     3961 tgaattgatt gttgagcgat tccagtttct tcagataaaa tccttctaag tcttgttgaa
     4021 tctaaagctg ttccagttcc aatgactttg tttttaggaa agccagattt ttcccaaata
     4081 taatttgtaa ctacatcaac aggattagat atgactacaa atataccatc aaagccagaa
     4141 tcaactatgg gttttactat agattttatc attccaatag ttggtcttag taaatctaat
     4201 ctagttgtgt tttgtctacg taaaggccct tctgaagagt ctgcaacact tatcactact
     4261 atgtcagaat ctttacagtc agaaaaagtt cctttttcta tatgtacttt atgtggcaga
     4321 tatgaaactg catcagctaa atctaaagct tgagcttttg atttagattc atctatatca
     4381 atcataaaga gttcatcaca aactccttga gttattaaac taaatccaca atgagaacca
     4441 acatgccctg aacctacaat agatattttt cttggcttta ttgccatttt aaatcactcc
     4501 ccctaaaata acgatacatt gaaaattata gatgttatta aaagctgttt ttgaatattt
     4561 taatattcta ttatttcaga aaatttattt aatatgattt tatactaaaa tttttaaaaa
     4621 agcaatgtta atatactctc tattagtgaa tattaaagta ttatatgtat attaataatt
     4681 attaaaatat gtaatgtcac tgatagttag aatatttaag tgataaaaga tgtgtataaa
     4741 taataatgat gtcagtttat aatgatatta aattgaggta tactgtttct ctaaattaaa
     4801 aaataaagga gtaaatgtgt tatgaattat catacaaaag aagaattggt ggaagcactg
     4861 agagtaatat cttcaagtat tattaattgt gaaaaaggac agaagaaatt ttctgaggag
     4921 acatctcatc atacatgttt taagaatatt atagaagcaa tatatatttc aaaatcatta
     4981 ataatggatg aaattagtaa aagagattga agcttatttt gcaatttaac atgttttaat
     5041 atacatttta ttatataaga aaataagtaa atatacatgt tataaaatat tggaataata
     5101 tacaattata aatttgttaa cattaatatc tatatgttaa aatatagtag aagatgttag
     5161 taaataatag gatgagttct attttaatac aattaaatta agcttagttt gaaatttgta
     5221 tctaaaaagt aaagtaatct agaattaaga aaatggagat agaattataa tggaaaaatt
     5281 atattataca aatcagtata taagagattt tacagcagaa ataattgaaa taaaagaagt
     5341 agaaaataag tatcatgttt tattagataa gacagctttt tttcctggtg gaggaggtca
     5401 gtttggagac ctgggaaaaa tagagaatga tgatgtaata aatgtgtatg aagatggaaa
     5461 aaaaatatac catgtattag ataaaaaacc tataaagata cataaggtaa agtgtagtat
     5521 tgattgggat agaagagaag atggaatgca tcaacatttt ggacaacatg tattgtctgg
     5581 atgttttttt aaaactttta atgcaaatac gacaggtttt catttgggaa atgagtatag
     5641 tacagtagat atagaaggta atttagaaga ggataaaatt agagagatag agttgtttgc
     5701 caatgaaatt attcgtcaaa atatacaagt agagacatta cttccaacga aaaaagaact
     5761 aaaaaagatt tggttaagac gtgatttgcc aaatactgat gaagagataa gagtaataaa
     5821 aataggtgat ttagatacga atgcatgttg tggagtgcat ccaaaatcaa ctggtgaatt
     5881 gagaatgata aaaataaaga aatgggaaaa acataagtca agtacaagaa tagagtttct
     5941 agcaggtaaa agggcagtgg atgaagtatt gaagagggat atatatttga ctaaaatatg
     6001 tagatattta agctgtagtg aggaagatgc tataaatggt attgtaaatc taaatagtaa
     6061 agtggaagag gctttaatta aaaaaagaag gcttgaagaa gtagtatcta catatcaggt
     6121 caaagaaatg attgaaaaag caaataaggt tggcaatatt tcggtcatta aaaaaattta
     6181 tgatgatgaa gatttgaaat atgtaaataa gatagcaaat aaaattgcag aatatgaaaa
     6241 tagtattgca ttattagctg ttaaatttaa tgataaaata aatttagttt ttgcatgttc
     6301 taaaaattta aaagatatta atatgggagt tttattaaag gatgcaataa atcttataga
     6361 tggcaagggt ggtggaagta aagtattagc tcaaggagga ggtaagaata atggaaattt
     6421 agaggctaca cttgattatg catttatgaa gatagaaaaa actctataaa tatattatat
     6481 aaaaaacctt agtagataaa atagatatgg aggaataagt atggagcaac aaaaggacta
     6541 catagagtta tatgaaaatg aagaactaat agagataaga gaagcacgta tggatgattt
     6601 ggatacaata gcaaaattta attataattt ggctaaagaa actgaaggaa aagaacttga
     6661 tatggatgtt ttaactaaag gtgtaaaagc attgttatta gatgagagaa aaggaaaata
     6721 tcatgtatat acagtatttg ataaagttgt tgcacagatt atgtatacat atgagtggag
     6781 tgattggaga aatggaaatt tcctttggat tcagagtgta tatgtagata aggaatatag
     6841 aagaaaaggt atctttaatt atttatttaa ttatataaaa aatatatgtg ataaagatga
     6901 aaatatagtt ggaatgagat tatatgtaga aaaagaaaac ataaatgcaa aagcaacata
     6961 tgagtcttta aatatgtatg agtgtgatta taatatgtat gaatacgaag taatacattc
     7021 gtaatataaa tttgtaatac tgttatactg atgttataaa gtattgatta tattgacgat
     7081 tatatgaaat aaactggata ttataattag aatttttagg gggtataaaa atgggcgttt
     7141 taaaaggaat gttttatgta tttttatttt tagatttagt gtcaatattc tgtttctttt
     7201 ttaataaagg aaaaatagca tctaataaaa ttgtgtttaa tgcaatagga gtacttacat
     7261 ttgtactttg ttttatgctt tttagttatt attctaataa taatcttatt ggaaaattta
     7321 ttgcttctct attttttata tttggagttg caggagttat gttgaaagaa aaaaattttt
     7381 tatatgctag acttctttta actgtagtta tagttttttc tacattaaga ttgttcgtta
     7441 ttcaataaaa tgtatttgta tgaaaattta ttaaagtcta cacatttaag tgtaggcttt
     7501 tttgattttt tgtttgaaaa ttaaggaaat tttaaggaaa ttttaaaatc gtcttaaata
     7561 tttattgata tacttaaaat attaatagcg aaaggagact tatcagtgat agaaagtaaa
     7621 aagtcagaaa aattgttact gtcaattata tacataatat ttttggtata tattttaatg
     7681 attataagaa taattttatt tagagatgta ccaatttacg ctattttcaa gggaacgttt
     7741 aggaatgtaa atttaattcc attttataca atatatcaat ttatagtaga tagtaatctt
     7801 gactttatga aagctactgt aaatattata gaaaatatag gtatatttat tcctatggga
     7861 atttttttac ctatagtatg taaaaatcta aataaaaaaa ctataattat agtaattata
     7921 cttgttagcc tagcatttga attaacacaa tatatttttg ctcttggaag tagtgatata
     7981 gatgatgtta ttttaaattc attaggtggc ataattggaa ttaatattta cataaacatg
     8041 aataaattat ttcccaatga tattaacaga tttaaggcta taataggtac tagtttagtt
     8101 ttaggagtta taggattagg agttatttct aaaaattatc ataacttact aacttttaaa
     8161 tttagacctg atagaaaaat atcaaaaata ttaattgaag aaaataggga aataattaaa
     8221 gatataaata aagatacagt agatatagtt gggacatttg agagctttaa aaaaggaata
     8281 ataactatta aagcaggttc taataacaaa gtaaagagac cagagaattt aattgattct
     8341 gatggaaata taagaattta tttaaatgaa aatacaaaat tagtatctta tataattacc
     8401 aaagaatcaa aaatggatat agttaaatat gaggaatttg atgtgaagaa tttagattta
     8461 ttaaggagat atgatactat taatgtttgg attgataaag aaaatcagcc aaaggataaa
     8521 catggtatta tggctagtaa attattaatt ggattgtatg aataaaatat taaacaagaa
     8581 taaaagaata acaaatataa aaaacgaaaa aactatttct ataattattt ttacgttgtg
     8641 cataaaataa gaacattagt gaaggagtag agataaatta tggagataat aaaatatatc
     8701 ttgtactttt ttatatattc atttttagga tggacagtag aaagtatagg atgttctata
     8761 gcaagcaaaa gaataatcaa cagaggtttt ttaaatggtc caatatgccc tgtttatggt
     8821 tttggagcgg taattgtgat atcactattg ggaagattta ataatgtagt aatagtattt
     8881 ctattaggaa tgattttaac tacgatacta gaatatttta caggatttat tttagaaacc
     8941 ttatttcatg ctaaatggtg ggattattct gacagaaaat tcaatataaa gggtagagta
     9001 tgtttaaaaa atgcaatata ctttggtgta atgtctgttt taataataag atttatacat
     9061 ccatttataa aatattttgt aagtataatt ccatatagaa tattaatttc aatggctata
     9121 attacaacac tttggactat acttgatttg attgttacta ttataacact taaaaaatta
     9181 gatataaagt taaacttatt agacgatata attacagacc ttaatgatat aaatgtgaaa
     9241 ttggataaat ttgatagagg agaaatacaa gctttattta aaacaataaa taacgacgaa
     9301 ttagaagtta gagaaaaaat aaacaaaata aatagtaagt tggaccgtat agagtctaat
     9361 attattttac aaaaaagagt tataaaagct tttccacata taaaacataa aaaacaacaa
     9421 gagcaactag aacattttaa aaaaattatt aatgagaaaa ggtaattata caatttaatg
     9481 aagacgaata gaataatggt atttataaga tgattttcag aggactctat aaaaatagaa
     9541 tagagtcctt tattttttga aaaataatgt ttatttgata aaaataaaat atattgtttc
     9601 tgaataacaa atattttgaa aattataaaa acatatattg acaatttgtg tcaaaaatac
     9661 taacattaag tatataaaga attttatatg agaatatctt tataaatcgc aataatttta
     9721 aaacataaat ttatttaaag aggggatgat ttgccaatga gcgagataac tagtcaaatg
     9781 ataagtacag aagaaaaaaa atatgttgct aaaactcaaa agatacctta ttatccagta
     9841 gcatttaaat caggtgatgg tgcaatgcta tatgattatg aaggtaatga atatgtagat
     9901 tttttagcaa gtgcaggctc tgcaaatgta ggtcatggta ataaggaaat ctcacaagca
     9961 gttaaagaac aaatggatga tataacacaa tatacacttg catatttcca tagtgatcct
    10021 cctgtaaaat tagctgagaa gcttgtggaa atagcccctg gagataatga taaaaaagta
    10081 ctatacagtg caactggttc tgcttgtata gatgctgcta taaaattagc aagagggtat
    10141 actggaagaa caaagataat atctatgtgt gaatcttatc atggaagtac ttatggagct
    10201 atatctatat ctgctttaag tacaaatatg agaagaaaaa tgggtccatt gttaccagaa
    10261 gtttatcact tccattatcc tgataaaaat agaacagcta aagaatgttt agatgagata
    10321 gaatatgctt ttgcacacta tctaccagca gaagaggtag ctgcaatatt tatagagcca
    10381 attgcaggag atgcaggaat aatagtgcct ccagtagagt gggtacaagg tttaagtaaa
    10441 atatgtaaag aaaatggaat attacttgta agtgatgaaa tacaacaagg tatgggaaga
    10501 acaggtaagt ggtttggtat agaaaatttt ggtgttgaag cagaccttat agttcttgga
    10561 aaatctgttg gaggtggatt acctctagga gcagttgttg gaagaacaga aataatgcaa
    10621 agtcttgatg caccagcaca tttatttact ttagctggaa acacaacagt atgtgtggct
    10681 gctttaaaat ctatagagat aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg
    10741 ggagattata ttaaagcagg atttgaaaaa ctaaaagaaa aatacgatat aattggagaa
    10801 ataagaggca taggattatc tataggagtt gatatagtta aaggaaaagg ttctaatgaa
    10861 aaacatccag atgctacagc taagatatgt tatagatgta tacaaactgg attaataatg
    10921 atattcttag gacaatctac tttaagagtg caaccacctc tagtaataac taaggagcaa
    10981 gtagataaag caatgaatat catagattct gcaatagatg attatttaaa tggtagaatt
    11041 ggtgatgaag tatatgaagt aactcaaggt tggtaaaata aatattaaat gaaaatagga
    11101 gttatctcaa ttttagaaaa ttatttctga aaagagataa ctcttttaag ttacccacaa
    11161 ataagaaaat tgtttgattc attaaagact atatttcgga tgtcgttttt gtgagtatca
    11221 gagacagtat tacgagaata attaccttta ttcgtactat ctgttaattt agttataatt
    11281 aagatagcta aaagaataag aattactgtg tataaagcaa taacatatgg ttttctttta
    11341 tatttgtatt tgttattgaa aaaagaatgg ccataattat ttttatttac tttagatgta
    11401 cttgtattct ttttcttgtt acctttcttt ttatcatcct tttttctatt tttagggtaa
    11461 ggaatgactt tgtgtgtatt atcaagtttg atatttttat ttttttgaat attgttttcc
    11521 aggctagcct ttttttctcc aaattgaata gttttattgg tattgttgtt atctttcaat
    11581 gttaatctcc ctcccttaaa ctttcccttt agataatatt actactaacc tatggaatat
    11641 acaataaata atatataagt aataaaattt tataggtata aatctctttc tccacgctct
    11701 aaggcagtca tattgttaga tactatgttt cttatatctt ctctttctat cttacctatt
    11761 tcttctgcga taagtttttt acccataatt ttaagttctt catctccata atcgattaaa
    11821 aattcattta atgtagtaag agcatttggt gtacatacat tatggatttg tcgactttta
    11881 gctaagctca tgaatctatc gccagttcta cctttacgat aacaagctgt acaataacta
    11941 ggtatatatc catcagtaat cagttcttta agcacctcaa cagggcttct atggtctcca
    12001 acttcaaatt gttctgtatt gtcaccatct tcatatgctt tatatcctcc aacacctgtt
    12061 agagaacctg cactaatttg tgatacacca tattttagta actcatttct catttcagca
    12121 gtttctctag tggacattat gattccagta aaaggaacag ccaatctagt tatagctact
    12181 atttttttga acatatcgtc agaaactaag tgaggaaaat cttctaagct cataccttca
    12241 gcttttttca atctagggaa agatatagta tggaaaccta caccaaattt ttcctctaag
    12301 tgttcgttgt gcatcataag tcctagaact tcaaatttag ggtcagataa accaaataaa
    12361 actcctgcac caacatcatc aacaccagct tccatagctc tatcaaaagc tgttaaatgg
    12421 taataatagt cattttttat agattgacca tgcattttta taaatgttgg tttgtgataa
    12481 gtttcttgga ataatatata agttcctatt cctttttctt ttagtaattt atactcatct
    12541 acacttgtag ctgctatgtt aacgtttacc cttcttatat ttccgttttc attatatgtt
    12601 gaataaattg catctaagca atctaagatg taatttatat cacaattttt agggtcttca
    12661 ccagcttcta gagcaagtct tttatgaccc attttttcta atattttaac ctcttccttt
    12721 atctcttcca tggtaagttt ttttctttta aatttgttgc atttactaaa accgcaataa
    12781 acacattcat ttacacagta gtttgataca taaagagggg caaataaaac cactctatta
    12841 ccatatattt cattttttat ttgcccagct atactaaata atctatctag ttgtttttta
    12901 tcttctactt caagaagtgt agctatatct ttataactaa gtttttccct tctatcagcc
    12961 ttatcaagta ccttttcaat atcatcagaa gttgaatttt ttgcatcttc taataaagaa
    13021 ttaataagtt catggtttat gaacatttac aaacgcctcc attcaatatt atcaccgcga
    13081 gaatagtcaa cttcaagtcc taatgacttg atgttttctt ctaaactttg gtgatattca
    13141 caagcttctt gaccagtaaa ggctttgtta tcgtaaagag aatattgttt tctaaattct
    13201 tgaggagaaa ggtttggcat aattacattg caaccagcta aaagacctgc atttctacca
    13261 ctagggttta tagatgccaa ggctgttgta gcgggtaata aaacttttgg aagtagaagt
    13321 cttgtaattg aaagcataag taatgtcttc tctaaatctc catgtttata atccttaaaa
    13381 attgtatcat ggtgtggaat aaaaggtcct attccaacca tatgaggatt taactctttt
    13441 aggtataata aatcacgaac aaggtcttcg ttgttctgaa atggtgattc aaccataaat
    13501 ccagcaccta cctgataacc aatttcttta aggtttttta ggcattcttt tctagttcta
    13561 agctcaatgt ttggcggatg taatttttta taatgactgt ctgtagcagt ttcatgtctt
    13621 aaaagatatc tatctgcacc acataaaaag tattttttat acgaatcata actcttttct
    13681 cctagtgata gagttatggc acaatcctca tatttatttt ttatacttga aacaatttca
    13741 catattttat catcagtaaa atatgcatct tcaccacctt gcaaaacgaa tgttctgtat
    13801 cctattttat aacctatatc acaacattca agtatttcat caagtgaaag cctgtatctt
    13861 atgatatttt tattagaaga ttgtattcca caatagtagc aattattttt acaatagttt
    13921 gtaagctcta taagtcctct caagtagact ctatttccat aatgtttatt tctcatgtcg
    13981 ctagcttgtt tataaagata gtttcttaaa ttttgttcga agttatattc ctcattgtcg
    14041 atattttcta ttaaatatag tagttcatca aaacttaatg aattaatgct gtaaagttta
    14101 tctataaagt atttaatctt atttttagtc atattcaaat atgtaattat taagtatagt
    14161 taaattttta ataattgaaa agttcaagtg atctttccaa aatacctttt acatatgcta
    14221 ttaagacacc atagtttaca ataggtacat tgaaagattt tgccttttct attctagata
    14281 acattccagc tctattcatc atacaagctc cacagtgaac tacaagggca tatttattta
    14341 tatcttcagt gaaggatact ccagaactaa attcaaaagt aatgtttttg ccagttttct
    14401 ttctaatcat attaggaatt tttactgttc caatatcatc agtttgtcta tgatgggtac
    14461 atccttcggc catcaagatt ttatcaccat cttttaaatt ttctaaagca taagctccat
    14521 taatcaattc tttaaggtct cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag
    14581 gaatatcttt tggtgtatct ttatctactt gtggaaatac ttgagagtca gtaataacaa
    14641 gttttggttt tttgcctaaa ttagatagag tttcttttaa actatcttct tttgtaacta
    14701 tagaaattgc accattatcc aaaatatctc ttataacttg ctgttgagga agtataagtc
    14761 taccttttgg agctgcttta tctattggaa caactaaaac aactaaatca tttggttcaa
    14821 ttaaatctga taccaatttg aactcagtgc tatccttagg caaaactttt attatttcat
    14881 tttttaaatt ttcaattcca attttatctg ttgatgagac agaaacaact ggacatttaa
    14941 ctttattttt agttaagttt aaaatttctg attgattctt aatagtatca attttgttaa
    15001 gaattagaat atgagggata ttcttatcat taaatttttc aattagtgac aaatcttctt
    15061 gtgatatacc aatttggcaa tccactacta gtaaagctat atctgtcttt tctaaaacat
    15121 ctagtgattt acctattcta agttcaccaa gttcaccaac atcatcaaga ccagcagtat
    15181 ctataagaac acaaggtcct ataggaagga tttccattgg tctaaatact gggtctgtag
    15241 tagttccagc aatatctgag actattgcag cactttgatt tgttatagca tttattatgc
    15301 tagatttacc tgcatttctt ttaccaaaga gacctatatg tactcttacc gattgtggag
    15361 tagaatttag actcatttgt actcaccacc ttaaacttct ttttttgata tactagtttt
    15421 tacagataca tgaggtatgt tgcccaattt accagtcaaa ctattaatag tatccatttc
    15481 gccaacaact gctatacata cgattgagac accttcttct tcgcaaggaa tgcccattct
    15541 tcctttaata atacctttaa aacctgatac aacctcattg aatttaaatt gacattcttt
    15601 tggctcttcc aaaatagcac ttataactgc aacctttttc ataaatatcc tcctaactca
    15661 gaaaatatgt cttaaacaaa tccaaaatca aaaaactttt ctttgtaaag aaaagtttct
    15721 acaaaagaaa agttattaat tacacaagta taatgtgcta tattaaacta tttccttaca
    15781 gattagataa ctcttttctg gtagggttag cttgtaaaaa tttaatttaa caacaagtaa
    15841 ctgaagaaaa tttttataca atttatataa attataataa aaattaacta aagaaaattg
    15901 aagttactta tataatagta gtaaattgaa attaagtcta tagaaaaacc aatttttgta
    15961 taaaaataaa aattaaagtt aattatttgt taagaattga ctttggtaaa atatataatt
    16021 aattattctt taagcatata atttataagt aattttagtg tattttcaat agcttctata
    16081 tgtgttcttt cataatggtg actattgttt gttccaggac caacacatgc tatttgaaca
    16141 tcttctcccc aacgaatagc ttctgaagca tctgaaccat agaaagtaaa tacatcgaca
    16201 gcgtagtcta aattattgtc ttctgcaaca tcgacaagct tgtttcttaa attaaaatca
    16261 tataatgata aattatcttt tgcaactata gttacaccat gttcactaga tgtttgactt
    16321 ggtccagtag gtgctatatc tattgctaca aattcttttg ttttttcagg taaacattta
    16381 gaaacaccat gtccaatttc ttcataatta cttatataaa agttagtagt atgcttaggt
    16441 gttaaattgt tttctttaaa atatctacat atttcaagaa ccattgctac agctgattta
    16501 ttatctatat atctagattt tataaaacca gattctgtag cctcaaatct atggtctagg
    16561 catacaaaat ccccaacact tattcctaaa tctaatacat cttgctttgt aaatactttt
    16621 tcatcaagtc taataatcat tgtttcttca tttctttcag tggcagcttt tgtttgacca
    16681 tatatatgag tagaagcata cttaaataca acacttcctc tgattttttt accttttcta
    16741 gttataacag tacagttttc tccctctata gctgcccaac atccaccacc aactttatgg
    16801 tatttaagag taccatcaga tgatatttct tttactatag ctccaagtgt atccatatgt
    16861 gcagatattg ttatttgatt tgaatcatct tcaccaggaa gagtagctat aagtgcacct
    16921 ttttttgtaa gaatagtgtc aagacctaaa ttttcaaaat cttttttgat ttctaaaact
    16981 gcattttcag tatatccagc tgggcttgga atgtccaaat attttttcat taaatttata
    17041 gtattagtta aatttaaatt catattgttt cctcctaaat aaatatattt atacaaatta
    17101 taacataaat acaaatgttc tgaaaactaa aattaaaaga atagaatcta aacatattta
    17161 aaaaaaaagt ata
//
LOCUS       DAENCV010000079        17027 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAENCV010000079 DAENCV010000000
VERSION     DAENCV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.080"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(4..1008)
                     /locus_tag="KQD82_002588"
     CDS             complement(4..1008)
                     /locus_tag="KQD82_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG4381050.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(1022..1924)
                     /locus_tag="KQD82_002589"
     CDS             complement(1022..1924)
                     /locus_tag="KQD82_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG4381051.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(2119..3294)
                     /locus_tag="KQD82_002590"
     CDS             complement(2119..3294)
                     /locus_tag="KQD82_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG4381052.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(3381..4226)
                     /locus_tag="KQD82_002591"
     CDS             complement(3381..4226)
                     /locus_tag="KQD82_002591"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG4381053.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(4324..5121)
                     /locus_tag="KQD82_002592"
     CDS             complement(4324..5121)
                     /locus_tag="KQD82_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG4381054.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(5140..6150)
                     /locus_tag="KQD82_002593"
     CDS             complement(5140..6150)
                     /locus_tag="KQD82_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG4381055.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(6169..6951)
                     /locus_tag="KQD82_002594"
     CDS             complement(6169..6951)
                     /locus_tag="KQD82_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG4381056.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(7010..8146)
                     /locus_tag="KQD82_002595"
     CDS             complement(7010..8146)
                     /locus_tag="KQD82_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG4381057.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(8854..9321)
                     /locus_tag="KQD82_002596"
     CDS             complement(8854..9321)
                     /locus_tag="KQD82_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG4381058.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(9400..9942)
                     /locus_tag="KQD82_002597"
     CDS             complement(9400..9942)
                     /locus_tag="KQD82_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381059.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(9994..10680)
                     /locus_tag="KQD82_002598"
     CDS             complement(9994..10680)
                     /locus_tag="KQD82_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG4381060.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(10680..11540)
                     /locus_tag="KQD82_002599"
     CDS             complement(10680..11540)
                     /locus_tag="KQD82_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381061.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            11675..12475
                     /locus_tag="KQD82_002600"
     CDS             11675..12475
                     /locus_tag="KQD82_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG4381062.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            12500..13069
                     /gene="yfcE"
                     /locus_tag="KQD82_002601"
     CDS             12500..13069
                     /gene="yfcE"
                     /locus_tag="KQD82_002601"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG4381063.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(13322..14374)
                     /locus_tag="KQD82_002602"
     CDS             complement(13322..14374)
                     /locus_tag="KQD82_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG4381064.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISHSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(14581..15807)
                     /gene="pepT"
                     /locus_tag="KQD82_002603"
     CDS             complement(14581..15807)
                     /gene="pepT"
                     /locus_tag="KQD82_002603"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG4381065.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(15898..16950)
                     /gene="ytvI"
                     /locus_tag="KQD82_002604"
     CDS             complement(15898..16950)
                     /gene="ytvI"
                     /locus_tag="KQD82_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG4381066.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
BASE COUNT         5416 a         3127 c         1842 g         6642 t
ORIGIN      
        1 aaattacttt tcttcataaa atgtaacatt ctttattatt atatctatag ttccatcttg
       61 attttgtttt acagtatact tcattgggtc ttcaaaatca gttagattcc cttttatgtc
      121 aaaaccatta tccgttttta tacttctttt tttaagcttt ttctcaaccc attttttatc
      181 tatactaaaa ctttcatcaa gacctttttc ttccatatgc tctttaaagc tatcctttaa
      241 ctcatcatct ttaattgaat tatcaacaaa gtcatttata tcaacttcat gtttttcctt
      301 cagtgtataa tttaatatac ttcttacatc ttcagcttgc tttatatcat tactaagagc
      361 attggttatc caattttcag ctgtattttt aaataccttg gtcttgtact tatcatcatc
      421 tattttttta gcatttaaaa attctgttac aaacttagat tctgaccctt ctttttcagc
      481 atccttatct aaaagcctta gatgaaactc atcatttata ccacttagac taatcaaagc
      541 tccttgcttt tgtcttaaag tctctgggat accaatttca tttgaaacca tttgaatatt
      601 aaatttatca tccacaaatt caattgaatg agtgtacaac tttttataat ctaatttcag
      661 tattgctaca ctcttttcat ctttaatagt atataagcaa attgccaaat cacaagattc
      721 tagtgcagca ttaattttca taacatcaaa caagtaagat gcaatttctt tagaattttt
      781 caaaaaagta ctttcatcat atattatttg ttcacaacag tttttaatta aattgtcatt
      841 ataatcttta aaaacacctt ttcttaaatc ctcatctttt gcaattcttt ttatagcttt
      901 ctggaaaaaa ccatccactt cttgattaac tttgccttca aagtcattta atattggaac
      961 atcactattt ttgtccaaaa catgtattat aaatttatgt attatcatat atatcacccc
     1021 attatcttaa tttcgatatt aactctttca tatcctctgg tatatctgct ttaaatttca
     1081 atttttcttt tgttcttggt tgttcaaact ctaacttata tgcatgaagt gcttgcctat
     1141 ttattagctc ttcatctaca taaccataca actcatcacc tattattcca tgacctatat
     1201 gagccatatg tactcttatt tgatgtgttc taccagtttc taagcttact tctaatacag
     1261 ttgcattttc tagtctttct attactttat agtgagtaac agatgactgt cctctttcat
     1321 ctattatcct ctttattgaa tcttctgtag gtctataaat tggctcatct atagttccct
     1381 cattttcttt tataattcca tcaactactg ttatatactt tttttcaact ttattttcac
     1441 tcattgctgt agacaaagta tgatgtgcat aagcattttt tgctactata actaaccctg
     1501 aagtattcat atctaaccta tttacaaatc ttatttttac attttctttt tgattatcta
     1561 tatagtaact aataccattt gctattgttt tatcatagtg gctttttgtt gggtgaacta
     1621 ccataaacgg aggtttattc accataatta tatcaaaatc atcataaatt atctgtaaat
     1681 ttaaatcttg aggttcaaaa tttgcttttt cctcgtctat ttttacttct attaaatcac
     1741 cactatacac ctttaaactt ggttttttat atactccatt tactaaaaca ctcttttccc
     1801 tcttcatttt tgataatgac ctaactgaga aatttagctt gtccaataaa acttctttta
     1861 aagtcatttc ttcttcattt gtatacgata taagattata tctttgattc tcttttttaa
     1921 acaaactttt acctactttc ttttatatct tcctactttt tatatcttca aaatcatgat
     1981 attatatatt ttacactatt tttgtttaat agataataat tatttttaac tataaaaacc
     2041 tgtaagaata acaaatagtt attcttacag gttaaattca taattaaata tttaatttgt
     2101 aaatattata atctaaaact atctcttaac tattaaagta gttcccattc caccgcctat
     2161 acaaagtgta gcaagaccag ttttagcatc tcttctcttc atttcatata aaagtgtagt
     2221 aagtattctt gctcctgagc atcctattgg atgtcctata gctattgctc caccattaac
     2281 attaacttta ttcatatcta tatttaagtc tcttattaca gctacagatt gggcagcaaa
     2341 tgcctcatta gcttcaacta aatctatatc ttcaatagtc atattagcag cttctaaagc
     2401 ttttttagtt gctggaactg gtccatatcc cattatttta gggtcaacac cagctgttcc
     2461 ataagaaact atagttgcaa gaggctctat tcctagttct tcagcttttt ctttagccat
     2521 tactactagc atagcagcac catcatttat tcctgatgca ttaccagcag taactgttcc
     2581 atctttttta aatgcaggtc ttaacttagc aagtttctcc attgtagtgc caggcttaat
     2641 atattcatct ttatctacta cagtgtcacc ttttcttcct tttataacaa caggaactat
     2701 ttcttcatca aattttcctt cagcttgagc tttttcagct ttattttgac ttgcaagagc
     2761 taattcatct tgttcttctc tagttatatt ccattgctct gctatgtttt cagcagtaat
     2821 acccatgtga tagttattaa atatgtctga taatccatct tttatcattg aatcaacaaa
     2881 agcagcatca cccattcttg caccatatct cgcacttggt actaaataag gagacatact
     2941 catgttttca gctccaccaa ctaacattat atcagcatca cctaatgcta taagttgaga
     3001 tgccattgaa acagatctta atccagaacc acaaactata tttatagtca tagctggttt
     3061 ttctactggt attcctgctc ctaatgctat ttgtcttgct atattttgtc caagacctgc
     3121 tgtaagtact ccccctaaaa gagattcatc tatcatatct ggagttatgt tagctctttt
     3181 tatagcttct ttagctgctg ttacccctaa ctctaccgct gaaactgatt taaatgctcc
     3241 tccaaaactt cctactgctg ttctagctgc actggcaatt actacttctc tcatattcat
     3301 tacccccatt aaattagatt tttcatatat ccgaaattgt tttttaaaca attttattta
     3361 aacttttaat tcccaaattc ttatttatta taatcataga atcctatctt agtttttctt
     3421 cctaattgat tagctctaac cattttagct aaaagtggat gaggtctata tttagtatct
     3481 ccaaattcag tatataaaac gttcattata gctaaaacaa catctaatcc gattaaatca
     3541 cctaatgcta gtggtcccat tggatggttt gctcctaatt tcatagcttc atctatttct
     3601 tctttacttg caacaccatc tgcatatata ccaacagctt catttatcat aggtataagt
     3661 attctattta ctacaaatcc aggagattca gatacatcta ctggtacttt attgatactc
     3721 ttagataatt caaatactgt atcaaaagta acttttgatg ttaactgacc acttataact
     3781 tcaactaatt tcatcatagg aactggatta aagaaatgca ttcctataac tttatctggt
     3841 ctcttagtag aagaagctat ttctgttata gataatgatg aagtatttgt tgccaagata
     3901 gtatcttctt tacataattc atctagtaac ttgaaaacat ctttctttat attcatgtct
     3961 tctacagatg cttctattat taaatccata tcttttaaat cttcataatt agtagttgaa
     4021 ctaacatgac ttaatatttc tgcttttgta gcttcatcca tttttccctt agtaactaac
     4081 ttagttaaat ttttatctaa taaagctaaa catttatcta tagcaccttg agttctactc
     4141 tttaaacata catcatgtcc acaacttgca aaagtttgta caataccact tcccatagtt
     4201 ccactaccta ttacagctaa tttcatagtt ataacctcct gttattttag tatttatatt
     4261 ctgttaacta gattaaaata ctatttaatc tagttaacag caaatatatt tttaattaga
     4321 tttttaccct tttataaagt tagcttctct cttttcaacg aaagctgaca ttccttcttt
     4381 ttggtcttta gttgaaaaac aaagaccaaa taaattagat tctatatcta ttccagtatt
     4441 tatatcagtt tgagcaccaa gttgtattgc ttctttagag tatctaactg caagctgagc
     4501 atttttagct attatcttag ctaatttctc aacttcttct attaaaatgt ctggctcaac
     4561 gactctattt actagcccta ttttttcagc ttcatcagct tttataactt gacctgtaaa
     4621 gattaattct tttgcttttg ccattccaac caatcttgta agcctttgag ttcctccata
     4681 tcctggagtt attccaagag ttacttctgg ctgaccaaat ttagctttag cagatgcaat
     4741 tcttatatca catgccattg caagttcaca tcctccacct aaagcaaatc cgtttacagc
     4801 agctatcact acttttttac tattttctat ttctccaaaa gcttttgctc ctaagatact
     4861 aaaatcttta gcagctacag catctaaatc tttcatgtat gcaatatctg ctccagctac
     4921 aaatgccttt ccttcccctg tcaatattac aacatcaata gtttcatcat tattaatatc
     4981 tacaaatact tcataaagtt cttctaaagt ctttgaattt attgcattaa gggctttagg
     5041 tctattcatt ttcactgtac atatatttcc atctacttca acagctacat tctcataaac
     5101 tttaacatca cttgtactca taataccctc cccaagaaat tagttagcta aaacttcacc
     5161 tttttctttt gcaacactta actgactgat aagttctgga agcactttat gaacatctcc
     5221 aactatacca acatcagcat atttaaatat tggagcttct ggatttttat ttatagcaac
     5281 tataaactca gcatcttcca taccagctat atgttgtatt gctccagata taccacatgc
     5341 tatataaagg tctggtctta cagttttacc agtttgacca acttgtcttg ctttatctaa
     5401 ccaacctgca tctatagtgg cacgagaacc agaaacttct ccacctataa tttcagctaa
     5461 ttcataaagt atgtctaagt tttcttttcc acccattcca cgtccagcag aaactaatat
     5521 cttagcatct tctattttaa cttgtttttt agcttctttt attacttgta caacttgaac
     5581 taatttatca gcatcattaa attctacctt gaaacggtta attacagctt ctttagtttc
     5641 atcaggttca tttttcttca taacccctgg tctaactgta gacatttgag gtctgaaatc
     5701 tttacaaact attgttgcca ttatatttcc accaaaggca ggtcttgtca ttaataataa
     5761 ttttgtatct tcagctactg caagacctgt acagtcagca gtaagacctg tatgtattct
     5821 agcagaaact ctaggcgcta aatctctacc tattgaagtt gcaccaaata atacaactat
     5881 agggtcagct gcttttattg cttcataagc tgcttttgta tatggttcag ttgtatacac
     5941 tgctaaagct tcatcatcta ctactattac ctcatctgca ccatagtgtg ctaatgtatc
     6001 tattaaacct tctaccttac tacctaaaag taatgcagaa acttttgtat cataatcttt
     6061 tgctatttct gtagcctttc ctagtaattc taaagaaaca gtttgaatta cattttctct
     6121 ttgttctatt actactaaaa cgttacccat tctttatccc cccttaaatt atatgatata
     6181 cttctctttt aatttatcta tgataattcc agctgatgtt ttcgcatctt cattgtatat
     6241 tgtaccagct ggtttaactg attttgtaaa tgatttaaat acactagttg gagaaccttt
     6301 aagacctaaa ttagaagggt caacttctat atcttttaca gtccatgttt ctactacatc
     6361 attttcgaaa gcatcatata ttcttccaac tttcatgtat cttggtgtgt tcatatcttt
     6421 aagagttgtt ataaggcatg gcattttaac ttttaagtca tggcaacaat cttcaaattg
     6481 tctttttact aatacatatt caccttcagt ttttatttct tcagcatatg ttattgatgg
     6541 aagatttaaa tgttcagcta tttgaggtcc aacttgtgca gtatctccat ctatcgcctg
     6601 tcttccagct attataatat caaaatctat attttttaat gctcctgcta atgctgatga
     6661 agttgcccaa gtatcagcac ctgcaaatgc tctatctgtt aataatatac ctctatctgc
     6721 acccattgct aaagcttctt ttaaagccat atctgcttga ggaggtccca ttgttataac
     6781 agttacatga gcacccattt cttcttttaa ttttatagct tcttctaaac ctgctttatc
     6841 atcagggttt attatacttg gtactccatc tctaattaaa gtacctgtat taggatctag
     6901 tttaacttct gttgtatctg gaacttgttt tatacaaacg actatattca taaatccttc
     6961 ctccatatct attttaatat cgacctattg tgtatatttt aatatttatc tattttaata
     7021 gttttcctga aataaccatt ctttgaactt cactagttcc ttcatatatt tcagttatct
     7081 tagcatctct catcattctt tctactggat agtcacgagt gtatccatat cctccatgaa
     7141 gttgtacagc ttttgtagta acttccatag ctgtttcagc tgcaaataat tttgccattg
     7201 ctgcttctac tccataaggt tttcctaagt ctttatttat agctgcttga tatacaaggt
     7261 gtctagccgc ttgtacctta acttccatat cagctaattg gaattgtgta ttttggaatt
     7321 ttgataatgg tctaccaaat tgtactcttt cttttacata tttaacagtt tcatcaagag
     7381 caccttgtgc taaacctaaa gcttgtgcag ctataccaat tctaccacca tcaagagtag
     7441 acattgctat cttaaatcct tgaccttctt ttccaagtaa attttcttta ggtattctgc
     7501 aatcctcaaa tattaattca ctcgtagctg aacctcttat acccattttc ttttctttaa
     7561 ctccaaagct aaacccagga gttccttttt caactataaa tgctgatatt cctttgttcc
     7621 ccttagattt atcagtcatt gccattacta catatatgtc accagctatt gcgtttgtta
     7681 taaatatttt tgagccatta agtatgtatt catccccgtc taaaacagca gttgtttgtt
     7741 ggccagacgc atctgtacca gcattaggct cagtaagacc aaatgctcct aatttttctc
     7801 cacttgctag tggtcttaag aatttttgtt tttgttcttc attaccatat tgatatatag
     7861 gccatgagcc aagagatgta tgagctgata atataactcc tgtagtacca caaactctag
     7921 acaattcttc aactgccatt atatatccta cagtgtctcc accttctcca ccatattctt
     7981 ttggatatgg tatacccatc attcctgctt ttgccatttt ttccactgtt tcataaggaa
     8041 atctttcttc ttcatcaagt tctgttgcta aaggtttaac ttcattttca gcgaagctta
     8101 catatagctc tttaagcatc tgatattttt tagaatttaa atccataata gcctcctcag
     8161 tattttataa atattttatt ttcatttatt aattaaaatc tcggcaacat ctataataag
     8221 gtatttgaat atacgtcgag aaatttataa ccttaatcaa actacgctct atatttatat
     8281 gtgattatac taaccaatta tgacattaat ttaatcataa attgttaatt taaaatcaac
     8341 tatactaaaa aaaatctcgc tttttggaat tttgtgaaat ttcaagttag aataagattt
     8401 ctttcacact ggttagagat aaaataattt gtttacgata tttatattag ggtatattaa
     8461 acattaaata ttgtctgtac atctgttttt tagccatgta cagaagattt ttatcgtata
     8521 aaaattattt taatagagct aaataatcat atttttatct gactattttt tatttttaaa
     8581 tatcttacat ctataatagt atcactcaaa ggataatttt tcaataattt ctaattattt
     8641 ttttaacaat atttattaca ttttttccac ttttttgtca tttttttatt gtttcgaaaa
     8701 aaaatcagaa ttttttaact ttattttgga ttattagttt aaattagatt ttcctcttag
     8761 atttataaat atttacacaa aaaaactacc tctgtataga ttgtatacag aggtagtttt
     8821 ttactaattc ctactatagt ttactatatt tttttaccaa ttcttacaaa catctaccca
     8881 tttttcacac ttagaaaact tctccatttc ttcacatgta agttctattg cagagttact
     8941 actgccacat gcaggaaata ctgtatcaaa tcttttcatt gaatcatcta aatagacttt
     9001 tatagaattt tttaaaccaa atggacagac tccaccaata gcatgtccag taaactccaa
     9061 gacttcttct ggtgtcaaca ttttagcctt acaattaaat tcagccttat acttcttatt
     9121 atcaatctta gcatcaccag ctgtcacaat taaaattgca tcatctccta ttttaaatga
     9181 gagtgtctta gctattcttg ctggaataac cccagctgct tcagctgcaa gttccacagt
     9241 agcactagat tgttcaaatt ctaagatact gtcttccttg ccaaattgtt taaaatactc
     9301 tcttacatta cttattgaca ttaaataccc ctcctagtct gaacttttat aaatttcata
     9361 ttattcttaa ttatatctat aattgattta tttttcaaat tattccataa tattagcatt
     9421 ccatgtatca ttattttttc caactactag ataaagtcct tttgccattg gaatattgac
     9481 aatatattct cctttatact ctacactcat accagattca ttaaagtacc tataatcaaa
     9541 atttagtcct tttggaatta caaaatcaac ataaccttga ttatttggtt ttattccata
     9601 tttcagagtt ctttcagtta tagaattttt ttcattaaat aaatctacat ttacatatgc
     9661 agaacactta ttcattccct ttttattttc cacacaattt tcaaacttca ttttagcact
     9721 gtttaattta taactatatt tactattatt gcttatttta aatcttacaa tagtaccaac
     9781 atctctactt ctcctgctat caaaatcaaa aacaatttcc tctaaatgat ttatttcata
     9841 atctttgaca cttaccatat ctctatatac taatttattt atagctatga atagtgtaaa
     9901 aatccccaaa ataaaaaatg aaacttttat aaacttcttc atttttagtc tccttatatt
     9961 ttgtgctatt gtgctataat attaatcaaa attttaataa ttaaacttat tcttttttga
    10021 aatcttaata gaaaaataca ttgatattat aaaaattatt attgttaaaa atgtcatact
    10081 atataaattt atatacttag tattagctat atcagttaca taatttgaaa gatatactcc
    10141 tccaacttta ctataattaa aatgctttac ttgatataca atcatactaa tcatcttcaa
    10201 aagacttcca tatacaaagt aaattaaaat acttactata aaaccacaaa ctttcatttt
    10261 tccatatcca aacttaaaaa ttaaaggcag taaaatcgtc aaaattgcta aaaatatatt
    10321 tacagaaaaa aatacatcat ttaaaaccat atttctaaag aacactagtg atgtcaattt
    10381 acttagtaag gacacaatta ttgttacaga aattattaat cctacaccta ataaatattt
    10441 tgagtaaact atatcttcta tactaactgg catactcctt ataaaaatcc ttgaatcatt
    10501 ttcataatca cactcaaacg tatttataaa tatcaaataa ctaacaacaa tacttactgt
    10561 ataatatgaa attgaattaa ggaaaaaata aaacacaaaa aatagcaata tgtactttaa
    10621 attacggata ccatctgaac taatccctat tattaaatct tttcttatta aattgaacat
    10681 catctcacct catttacata ataatacatt agttcttcta tagtaggatt tttgacctta
    10741 tgcttatcaa tttctatcaa taggcttttt actttcaagc tatctccaac tttaacaagt
    10801 gcctcattat aatatttata ctctttcaca ccaactatgc tttctttaat tgcttctaac
    10861 tctgctttat ttgccctaat tatcttatat tcttgttcta ttaattcttt cttaacagta
    10921 aaaatctgat ttcctctatt tataaaaaca agataatcgg caattttaat taaattatca
    10981 gtattatgag tagaaattat tacactacta cttccatttt tcacaaaatc ttgtaagtac
    11041 tgcattattt cagttcttac aattgggtct atcccagcag ttggttcatc caaaatcaat
    11101 aacttagcat tgtgactaag tgcacttgat aacataagct tttcagattg acctttagat
    11161 aaattttcaa tatgcatatt ttcttctata tcaaacttat taaggtattc atcaaacctt
    11221 tctctatcaa aattcttata aaataaagaa actatttttc taaattcctt aacctttaaa
    11281 tgcggataaa attgtaaaga atcatataca aaaccaatat tgtttttaaa acttattgga
    11341 gaattttcaa tagtttcatt acaaagttca atttcacctt catctgcatc aataatcccc
    11401 attataattt ttattaaagt cgtttttccc gaaccatttg gtcctataat cccaacaata
    11461 tccccttcct ttaactctaa gtttatatta tttaacttga attttcctaa tttttttgtg
    11521 atattcctta tgctaaacac atcttcacct cttatgcatt tttttatacc cacctataca
    11581 tattttaaca cattcattac aaaggtcatt tataaattta taaattaaga attataaaac
    11641 tatgttataa tacaaagtat gaagaggtga atatatgaaa agaattataa ctataaatac
    11701 aaaccaatta aataagtccc ttgagactaa agaactctta actagaaaac ttatcaatgc
    11761 tggctttgaa gtgtattcag atatttatcc agacacagag cttattatat caattggtgg
    11821 tgatggctct tttttaagaa cagttagaga tttcgatttt cctgagattc ccataatggg
    11881 aataaataca ggtcatctgg ggttttttcc tgatatttta ccagataaga ttgatagctt
    11941 tattgaagct tatacaaaaa aagattatat tattcaagaa atgtcacttc ttaatgcgga
    12001 agtatataca actactagtg gttctaatat gcttgctgta aatgaggttg ttataagagg
    12061 agacaagtct aggactatac acctaaattt aagccttgat aataaacata tacaaaattt
    12121 tagtggagat ggtatgatta tttctacttc cacaggttca acagcctata actattctgc
    12181 tggaggtagt attgttgata taaatcttga acttatgcag attacacctt tacatccgat
    12241 aaataccaat gcatataggt gctttacatc aagtataata tgttcaaatg aatctgttat
    12301 aaagattgca ccagaatata gatttgaaga ttctgtattg attgtcgttg atggagttga
    12361 gcacagattt agacaaatcg aaaatataaa agttagtata tctgatgcaa aaataaaatt
    12421 acttagaatg tcaaactatg agttttggca tagagtatct gaaaaatttt tatagactgt
    12481 acttaggagg atttttaata tgaaaatagg aataatgagt gatactcatg gtagcttact
    12541 ttactttgaa aaagcgttaa atgtattatc tgattgtgat gttttattac atggtggaga
    12601 tgtcttatat catggaccta gaaatgatat tccagaagga tataatccta aaaaatttat
    12661 tgagacatta aataaattag aaaacattgt gattgtaaaa gggaactgtg atgcagatgt
    12721 tgaccagatg gttatagaac atcctattca gagtccttat gtcatgagcc aatttggtga
    12781 aattagaatt atattgaatc atggttatat agaatcagaa gaagaaatta ttgacaaagc
    12841 aaagaaaatg ggtggtgaca tattagtttt aggacatact catgtaaaaa agctatacat
    12901 ggatgataat ttaatagtaa taaatccagg aagtacttct atcccaaaag atggaagcca
    12961 ttctgtcgca attatagata tcataaaaac tgatgatgag gatgagcttg aacttgatat
    13021 aaacttaatt gacatcaata ctggaaatat aataaatatc aatgattagt aaatttttaa
    13081 taaaaataaa ataagcttta atttattaaa ctcataataa aaacaagtaa accattttat
    13141 ttctatctca attagaagtt taagtacaat accttaaact tctaattaaa tagaatctgg
    13201 tttacttgtt ttattatatg ctatattgaa atataactta gtttacttat tttattatat
    13261 atattgaagt gcaacttggt ttacttgcta ttattatata ttaattttga tttacacaat
    13321 attactgtaa ggctaatgct tctgcatatg gtttactatt tttacctcca ccaacttgta
    13381 caaatttatc agcctttact ttttttataa aacttttctg tgcactactt aaacttccac
    13441 ttgcaagtac tacaggtgac tttgtttttg atgctaatac tccagcagca agcccatcaa
    13501 ctaaatcagc ttctctacca cttccatctt ttgcaaaata catattatta accttacttg
    13561 attttccata aaacttttct aaaactttcg cattagtatc ttttctatct gtccctgcta
    13621 gtctttctga gtttactaac tgtccttcaa gttttttact tatactgtac tccccaccta
    13681 ctatgtatgt cttagttgag ttttgtgtaa taaaattctt tgcctcactt cttatgctat
    13741 ccttacctgt atatattata gctgtattat cacttgcagc tgggcttgat attgaagttg
    13801 catctgccag tccattatac ccattaacca ctgccacttt tgagacacct aaatcaccag
    13861 tatcatctag ctgtctagta agaagtaaag atgtttcttc tctatctgct ccaccgattc
    13921 tactgactaa tacttccata tcttcaagag attcttttac acttgatgaa atagctcctt
    13981 cacttccaat taaaataact tctgctggat taagcctttt tatttcctct gttacattat
    14041 atggtatact atttttattt gtaagcagta ttggcgcatc caatttactt gccaaaggag
    14101 ttgaagccaa tgcatctggc aatgaatttg agttaactag aaacaccctt tctgaaccat
    14161 tattccaacc actatgagat atttttatcg ctgtatcaaa tctatcttca ccaataagtt
    14221 tttcctcctt tggagatttt ctaccttgaa gataattgtt catatctacc tgtgcatttt
    14281 gatttttact aatagtgtct gcatatacac aacttggcaa cataatccca agtgaaagtg
    14341 caaatgctat tattttttta gatttaattt tcaatacaat tccccctttt gaatgttaat
    14401 acctattgat attcatactt tttattgata ccctaaacta aaaataatta tcttttataa
    14461 aaataattac aaaaatagct ataccatttt aaatacaaca caagcttata atcagcttga
    14521 cttataaaga atgatatagc tattataact ttaaatgctt aatttttaaa taccttttaa
    14581 ctaaactctt tcagcatatt tttcagctat tcttacaatt agtttagtag ctttctccat
    14641 agaactaact ggtatacact catttttccc atggaagttt aatccaccag taaatatatt
    14701 tggacaaggt aatccattaa aagataatct tgctccatca gtaccacctc ttacaggaac
    14761 taaacgtggt tttataccta attcctccat agcttcttta gctatatcaa ctataaacat
    14821 aactggctca atcttttctt tcatattgta ataagaatct tttaaatcca attccactct
    14881 attatcgtat ttcttattta ttttttctat attctctatc ataatagatt ttctattttc
    14941 aaatttttct ctacaatggt ctctaataat ataagccata cttacattct cgacatttcc
    15001 acctatagaa tttaaatgcc aaaatccttc atatccttct gttgtttctg gtctttcatc
    15061 tgctgggaac atttctgata tttcagcagc tatatgtata gcatttacca ttttattttt
    15121 agctgaacct ggatgaacat ttctaccatg tatagtaata gttgcatttg cagcattaaa
    15181 attttcacat tcaagttctc caactattcc tccatctaaa gtatatgcat attttgctcc
    15241 aaatttttca acatcaaata aatcagcacc tctaccaatt tcttcatctg gtgtaaatcc
    15301 tatttttata tctccatgct taatttctgg atgatttatt agatactcta ttgcagtcac
    15361 tatctcagct ataccagctt tatcatcaga gccaagtaga gttgttccat cagtgactat
    15421 taaatcgtca cctttaaatt cttccaactc tggagaatcc tttacatacg taataacatt
    15481 taacgcttca tttaaaacta tgtcttttcc atcataattt tctattattc ttggtttaat
    15541 atcttttcca ctaacatctt caattgtatc taaatgtgat atgaatccta tagtatccac
    15601 accatcagta tttcctttta aagtagccat tatataacta ttttcatcta aactcacatc
    15661 atcaacaccc attgttttta gctcttcaac tatatactta gctaaaactc tttgaccttc
    15721 tgaagatgga cagttctcac actgagaatt agatgttgta tcaaatgata catattttaa
    15781 aaatctttcc actacttttt gtttcacgta tataccctct ttcttaataa gattaatatt
    15841 tttttattta cctgtgaata aaattttatt tttaaaaaaa tctatcaatt attataatta
    15901 aaaaatatcc accttcttaa gtatatcatg catacatacc aaacccaaac aaaaaattac
    15961 tccaattatt ccttttactt ggattccaat aaaaatcgct gctagggtag ctaatggatt
    16021 tagtccaaca tttactgata ctagtttagg ttcaagtatt tctctaatca aagacaaaac
    16081 aaaaaatatc acagtcatac ttatagctat aaaataattt tttataagaa aataataaac
    16141 aattactgga agatatattg ttattatacc taggaatgga attaaatcca ttaaagctcc
    16201 tacaaaccct actattagac cgtatttcaa tccaaataaa gcaaaacttc cccagattgc
    16261 aaaaaatgtt atagccataa gtattgtata ggcccttata tatccaacaa gtgaaagccc
    16321 tgcctctttc tttacgtttt ttactttctt tctaacatca acagtaaaca tattataaaa
    16381 actattttca atcttatcaa ggtcttttgc tataaagtac gtagcaataa acaaggtaat
    16441 tataaatatt ataatatatg gaatagaagt agccaatcca agtatactag taagaagatt
    16501 ttttgtaatt tgcattactt caccactgaa tttgtttact aattgttcta catcaaaatt
    16561 ggctatttct tgaaatgatt tcataaagtc atttatttta tttaaaaaat acataatagt
    16621 attacttata tcctcaggat tagcagatat attatttaaa aatcccatta attctttcat
    16681 tgaatttatt actattattg tagtaactgt actgactata gccactgcaa gaaaacttag
    16741 aacaagagta gagatacctt tatttatatg aaacttattt tctaactttt gtgatatagg
    16801 attaatcata agtgctatta tagcacctag aaaaaatgga gctatgtatg gaaacgcttt
    16861 gtaaataaaa ataaataaca atgtatatat acaaaaaaag atagtgttgt tttttaattt
    16921 gtataaaaaa ttattttcta gtatgctcat cttaaaaatc tcctttcttc taatttatat
    16981 ttttattttg tccatttcat actagtttta tttgaataca aaataaa
//
LOCUS       DAENCV010000080        16596 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAENCV010000080 DAENCV010000000
VERSION     DAENCV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.081"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            521..1381
                     /locus_tag="KQD82_002605"
     CDS             521..1381
                     /locus_tag="KQD82_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4381067.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1371..3242
                     /locus_tag="KQD82_002606"
     CDS             1371..3242
                     /locus_tag="KQD82_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4381068.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3262..4716
                     /locus_tag="KQD82_002607"
     CDS             3262..4716
                     /locus_tag="KQD82_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG4381069.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5741..6949
                     /locus_tag="KQD82_002608"
     CDS             5741..6949
                     /locus_tag="KQD82_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381070.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6980..8359
                     /locus_tag="KQD82_002609"
     CDS             6980..8359
                     /locus_tag="KQD82_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381071.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8465..9655
                     /locus_tag="KQD82_002610"
     CDS             8465..9655
                     /locus_tag="KQD82_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381072.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9783..11111)
                     /locus_tag="KQD82_002611"
     CDS             complement(9783..11111)
                     /locus_tag="KQD82_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381073.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIKIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11729..12943)
                     /locus_tag="KQD82_002612"
     CDS             complement(11729..12943)
                     /locus_tag="KQD82_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG4381074.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASYTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13369..14085
                     /locus_tag="KQD82_002613"
     CDS             13369..14085
                     /locus_tag="KQD82_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG4381075.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14220..14846
                     /locus_tag="KQD82_002614"
     CDS             14220..14846
                     /locus_tag="KQD82_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4381076.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15046..16590)
                     /locus_tag="KQD82_002615"
     CDS             complement(15046..16590)
                     /locus_tag="KQD82_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4381077.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
BASE COUNT         6148 a         1931 c         2759 g         5758 t
ORIGIN      
        1 ttaagtattt atatattagt atatcttact agcatttaaa tgaaactaaa tttgaagctt
       61 tacctattaa tatatcttaa tagtgattaa ataaaactaa acttaagact ttatatgtta
      121 atatatctta agaatttatt tgttaacata cagttcatta tatttaaaag cattactatg
      181 ataattcata atagtaaaat agataatggt tcagacaata ttataaatat tagtgacggt
      241 aaaatagtat caatttagta tataaacctt gtcagataat cgcataacag agcttgtagt
      301 tatagaaaaa aaaaatatct aggaaaccgt tgacagattt atatggatag tataacatat
      361 agaatatgaa attaaacaaa aaagaatttt aattatggcg acgtagtcat aattttgagt
      421 atgttactga ttagcaggca gaactcaagt atatatgtga aaaatagagg gatactctat
      481 acatatatgc ttgagttctt tttattgaag ggaggaatat atgaaaataa ttcagatttt
      541 aaataataat gtagccttag taaaaagagg taaaaatgaa attattgtat ttgttaaagg
      601 aattggcttt aaaaaaagag tgggacaagt aataaatgaa aatgaaatag agagaacata
      661 tatactagat tcatacgata tgctagaaca ctttagttat ctactacaaa actcagactc
      721 caataatata gttcttatta atgaaataat tgaatatggt gagaaagctt taaatataaa
      781 gtcaagtgat tatctaagct taaccttact agaccattta gagtttttac ttaaaagatg
      841 tgaaaagaat caatttataa aaagtccttt ggtatggaat gttaaaagat tttatccaaa
      901 gcattttgaa attggaatgc atgcactaaa attaattggt gatagtaagg gaattaatct
      961 tcctgatgat gaggcagtat caatagcact tcattttgta aatatggagg taaataaaga
     1021 gtctcatgat agtacaattg tagagttgag aactttagca gatatagttt ctataataaa
     1081 atatcatttt aatgtagagc tagatgaaac ttcaacaaat tatatgagat ttactactca
     1141 tctacagtac tttatccaaa gaattatgtc ggatgaagct tcaatagatg aagataattc
     1201 tatagattta tataaacaag taagtcaact ttatccaaag tcatttggaa gtgttcaaaa
     1261 gataagaaaa tatataaaat cacaatttaa tgtcgatata tctataagtg aagaaactta
     1321 tttaatgttg catataaata gggtgactca aagactggag gcaaaatcag atgaaatata
     1381 aagatttaaa tgaaaatata ataaaactta tagggggaaa agaaaatata aagtcggtag
     1441 cgcattgtgt tactcgttta agatttactt taaatgatag gagtatcgct caaacagaag
     1501 aaattaaaaa attaaaagga gtaattgatg tagtttctaa tgatgttgct tatcaaatta
     1561 taataggtac tcatgttgta gatgtatata acgagtttat gtctatgata ggtttaagtt
     1621 caagtgaatc aagtataaaa agtgaaagaa aaaagggaat tagaggtatc aaaggtatat
     1681 ttaattccat ctttgtggta gtctcagaga ctatgacttc agtaattgaa gtactacttg
     1741 cagctggtat acttgcaagt atattagcac ttttaaatat gtctggcata cttcagagtg
     1801 acagtcctac atatcaaata ttagatacat taagaagtgc tacatttcac ttcttaccag
     1861 tattaattgc aatagcatca gcaaaaagac ttaatgtaaa tccctatata gcagtagtgt
     1921 tagcagttac gttattatct tcatcaatag atggtgtaga aggattaagt ttatttggta
     1981 taaatttaca ggctacaact tatgcaaaca gttttattcc aatactttta ggagtatggg
     2041 caatgggaat tataattgaa ggtttgaaaa aaatattacc taaagcacta cactattttt
     2101 tagttccagt attatcttta ataataactc tatcagttac attaactgta tttggaccta
     2161 ttggtatgtg gattggtgat ggattaaact atgcatgtat gtttttaatt gatacattgg
     2221 gtaattggag tgttgttgca ctgtatgcag ctttacaacc actcttaata gtttttggtg
     2281 cagcaaactt tactatgcct atatctttaa attctgtgac aacattgggg tatgacccaa
     2341 tttttatggg cgctgcaaca atatctgata ttgcagtttg tggtgcaatg tttggatatt
     2401 tcttaaaaac taggaataaa gaacaaaaac aactatttgg tacagtaagt tttagtgcac
     2461 ttatgggagt tacagaacct tcagtgtttg gtgtgtttat gaaatataga agaccatttg
     2521 tagcagtaat gataggtgga ggtataggtg gtttaatagc aggtttagct caagttaaaa
     2581 catatggaat ggtttgggga ttagctgcat taccaacata tcttgttgga ggagaagtat
     2641 ctaactttac atttatgata ataagtgtag tagtatcatt tgtagcagca acagcagctt
     2701 cctatttact aggaatacct tcagatgaaa aagaagaagt taagggtgat aatgaaaaag
     2761 aattagaact aataatcaac gagaacaatt caagaactat tagtattgga aatgttgcaa
     2821 aaggtcaggt aattgcactt ggggaagtta atgacaaagc attttcatct ggagcattag
     2881 gtaaaggggt tggagttata cctagtgaat caaaatcaac tgttatatct cctgtagatg
     2941 gaactataac tatagtattt ccaactaaac atgcatatgg tatagtgact gatgaaggat
     3001 tagaaatatt aattcatatt gggatagata ctgtaaactt agatggaaaa ttctttgaaa
     3061 gtcttgtaac acaaaatcaa agggttaaaa aaggagatgc tttagcagta tttgaatctg
     3121 ataaaattat agaggaaggc tttgattcta ctataataac tgtagttaca aatactagcg
     3181 attatttaga tgtaatctca tcaaatgatg aatcagaaga attattaact gtaatagtat
     3241 aaattttagg agggaaaatt catgaatatt aaagatacat ttttctgggg aggaagtatt
     3301 gcagcccatc aatgtgaagg ttcatgggat agtgataaca aaggacctgc aattatggat
     3361 tttgtaacta agggctctta tgaaacgcca agagtaatta ctgataaaat agaagaaaaa
     3421 ttggattatc catctcataa tggaatagat ttttataata gatataaaga agatatagct
     3481 ttatttaaag aaatgggatt tagtgcatta agaatatcta tagattggtc aagaattttt
     3541 ccaaatggag atgatgaaaa tcctaatgaa ttagggatta aatactatga aggtctgata
     3601 gatacattaa tagaaaataa tatagaacct attgtaacac tatatcattt tgaattacct
     3661 atgaatttag tacataaata tggttcatgg aataacagaa aacttataga tttgtattta
     3721 aagtacagtg agactgtaat aagaagattt gatgataagg taaagtactg ggttacattt
     3781 aatgagatga accatattga ccctcaaaca gagcactcgg atatttttac ttatatgata
     3841 gcaggtatta agtatagtga aatagaaaat aaggttcaaa cattggcaaa tgtagggtat
     3901 aatatgactt tggcgggtgt aaaggttgca aagttggcta gagaaatcaa tcccaacaat
     3961 aaagttggat gtgtatttgg actaaatcca atttatccat acaattgtaa tcctagtaat
     4021 gtgttaaatg catttttaga taatgaccgt gattattatc aaattgatgc aatgtgcaat
     4081 gggaagtttc cacaatataa attaaaagaa tatgaaaaaa ataatataac tattgaagtg
     4141 actgatgaag acaaaaaagc atttaaagaa ggtaagttag attttatagg acttaattat
     4201 tatttttcaa gtgtatctac tccaaaagaa aatttatcag gtgataaatc tttatttggt
     4261 gggattcaaa atccatattt ggaacaaagt aagtggggat gggcaataga ccctataggt
     4321 ttaagattta ccttgaacta tatttacaga aagtatcaat tacctatctt aattacagaa
     4381 aatggtttag gggcatatga taaaatcgaa gaagatggaa ctataaatga tgattataga
     4441 attgaatatt taagtaagca tctatctcaa atgaagtctg caattgaaga ggataatgtt
     4501 gaatgttttg gatatttgat gtggggacca attgatttag tcagtgcaac tacaggtcag
     4561 atgagtaaga gatatggatt tatatatgtt gacttagatg atgaaggcaa aggaagtttt
     4621 aagagatata agaaaaaatc atttgattgg tacaaagaag taataaaatc taatggagaa
     4681 gttttagatt tttctacgga tatagtagta gggtaggaat aagataaata gaagttagga
     4741 actaatgtta attagtgtct agtaaagcat ttaaaataga gtatagggtc tttaaagtaa
     4801 agtgtgtatt cttcttaaaa gtaatggcaa ctaaaattac tataaaaagg agaatacaca
     4861 ttttttatgt ttatacttaa gtaaatattt agacatattc atagtataat ttactcatta
     4921 tataaaaata gtatgattta ctcactatat aactttataa attttctgga aaaatgtcag
     4981 aatatattga caaagaaaag atttaaaatt ataatctata ttaagaatag tttgacaata
     5041 taaaaacaag tatttttata aatagtgaaa ttatttttat tgtaaattta agaataattt
     5101 aggactacta taaagataaa ggtatagatg tcattatatg ataaatcata cttgtataga
     5161 aagaatttta aagtatggct tatcattgat ttaatataat aaataatttt tgtttctagt
     5221 aatagttcct aggaataaga tatctaaatt cgtttagaaa tagtctaatt tacaaaggag
     5281 gggatgcact aatagataaa atttctatat aaagaagact taaagtgaag tatattctga
     5341 taatattggt ttaatgggta tattattatt tcgttttaga tattttaaga ataatttaag
     5401 acgtttataa aaatgtattc tatatctaat atttaaataa atataagtct tccaacatgt
     5461 atactaatat agagagtagt tgaagtacaa ttctaactcg taaaattatg tagaaagtaa
     5521 aaatcaaaaa gaaatctagt aagttgataa ctatagttaa tatttagaga gttaactcta
     5581 gttatattta tttagatatt ttaggaataa tttaggacgt atataatatc atcaaaaaat
     5641 atcaagctat accatatgta gttaagaata actgcataat atacatttta taattattgt
     5701 tttattgata aaaaattatt atatttagga ggaaacaaca atggaaaatt tttggaatcc
     5761 acttacttgc tttactgtat gtgtaatgat ttatgctttt agtgagtttt tatctaagaa
     5821 aacaaaaggt gcaatttcaa cattgttatt tgtatgtatt atatttttac taggattttg
     5881 gacaaatatt ttaccaaaag acattacagg agcatcaggg atattagctg caatgagcaa
     5941 ctttggtata gctttattaa taactaactt aggaacactt ataaatcttg aagacttatg
     6001 tcatgaatgg aaaacggttg taattgcatt agtaggtatt gtaggaatag gggttggctg
     6061 tttaacagtg ggtagtatgt tatttggtag agagtatgct ttaatagcag cacctccaat
     6121 agcgggttct actgttgctg gtattatcgt aacaactgtt gcagaagctg caaatagacc
     6181 tgaacttgct gcatttgtaa ttctagtatt atcatttcaa aagttctttg gcattccgat
     6241 aactacatat tgtataaata aagaattaaa atttaaaaga gataacgggg attttttgaa
     6301 agatgagtca aaaagtaatt ttaaacttcc aagtatgaga atttttaaag aagatttagg
     6361 taaaaaatca tctactttag tatatcttgc aaagctttca tttgttgcta tgattggtaa
     6421 ttttgtagga ttggctactt taataccagg atttacccct gcaaactatt atttaaatcc
     6481 caacatagca tgtttgttag tagggttgat ttttacacga ataggttttt tagaaaaagg
     6541 aattttagat aaatctcaat ctaatggaat tataatgttt ggttgtatgt tgatgttacc
     6601 tggaggactt gctaaggtaa ctccaacagc attgtttggg atgattattc caactatagg
     6661 aatacttgtt gtaggctctg tatttataat actatttact gcaattgtag gaaaactatt
     6721 gggatattct ccaagaatgt cagctgcaat tggtgtaact tgtatgctag cttatcctgc
     6781 aacacagata atttccaatg aaggtattga tgctatggaa ggaactgaag aagaaaaaca
     6841 aagggcaaca gattatttat taccaaagat gattatagga ggatttgtta ctgtaaccat
     6901 tgcatcagtg gcatttgcta gtataatagg tccaattata tttggttgat agtaaataga
     6961 tttataggag gaatagaaaa tgataggaag tatatctata aatgagatag ataaaaaaag
     7021 attcaaactt gatgatttaa gtaagaaaat atgggaaaat ccagaaaaag catttaaaga
     7081 atttaaagca tgtgaaaata cagcaaactt tctaagaagt gaaggatttg atgtggaagt
     7141 aggtgtagga ggtttagcca cggctattag agctagtttt ggttcaggta aaccagttat
     7201 aggttttatg ggagaatttg atgcattacc tggtttaaat caaaaagttt caacaaaaca
     7261 agaagcattt gaattgggtg catatggtca tggatgtggg cataatttat tatgtacagc
     7321 acatgtaggg gcagtagtag ggcttaaaag agagatgata gaaaataact taagtgggac
     7381 tatagtattt tatgcatgtc caggagaaga acaattaaca ggtaaaggat ttatggctag
     7441 aggtggagcg tttgaagggt tagacttagc tataaatttt catccaaata aaattagtga
     7501 agctacaatt ggtacttcaa cagctgttaa ctctgtaaag tttcacttta agggaaagac
     7561 tgcacatgca ggctcagacc ctcaaaatgg aagaagtgca ctagatgctg tggaacttac
     7621 taatgtggga gctaattatt tgagagaaca tgttacttct gatgtaagga ttcattatac
     7681 aattacagat ggaggagtag cacccaacat agttcctgat aaagcatcag tatggtatta
     7741 tacaagagct ttaagtagag aagctgtaga aagtacatat gaaagacttg taaaggtagc
     7801 aaaaggagct gcaatgatga ctgaaactga agttgaagta gaatttttag gaggatgtta
     7861 taatacttta aacaatcatg ttttagcaaa tcttgtatca gaatgtatga atgagattaa
     7921 acaagagcct tggactcgag aggaattgga ttttgcagct gaattagata gacaaagccc
     7981 acaacaatat aaggctatga taagtaaata taatttgcca gaaggaactc atctatatta
     8041 tggtggagga aatgttacaa actttaatag ttatggctct actgatattg gagatgtaat
     8101 gcatatagtt ccgacagcat acttttttac agggtgtacg aatctaggtg caccaggtca
     8161 tagctggcag tttacatctt gtgcaggaag ttctatagga gaaaaaggta tgatatatgc
     8221 atcaaaaatt atggctatgt ttggagctaa aatattaaat aatccagaga tagctaaaaa
     8281 ggcaaaagaa gaatttgata aatctatgaa tggtaaaaca tataaatgtc ctattccaga
     8341 tgatattcca actccttaaa aagacatgaa gtttaatata taaaattata atttttatat
     8401 cagcatcatt tcattactaa aagaaagtga gattttgctg actatctgat taggaggaag
     8461 gaatatgaaa aattttttta aagaagcgca gtcaatagaa ggggaaatta ttagatggag
     8521 aaggtattta catgaaaatc ccgaaattgg ttgcaatcta ccgaaaacag taacctttgt
     8581 taccagtact ttagaaaaaa tgggatattc tccgaaattg acgtctaata atggaatagt
     8641 agctgtttta gacagtgaaa atggtggaaa gaccttatta ttaagggcag atatggatgc
     8701 acttcctatt atggaggaga cggatttacc ttttaaaagt aagaataaag atgcatctca
     8761 tagttgtgga cacgataatc acacagcaat gctcttagga gcagctaaaa tacttatgga
     8821 taataagcac ttacttaaag gtagagtaaa gtttgtattt gaaccagatg aagagactac
     8881 cagaggtgca aaatcaatga ttgatgatgg tatattagaa aatcctcagg tcgatgcagc
     8941 tatggcattt cacagtatgg tgggtaaatt tcttaacaca ggtcaagtag cttattcacg
     9001 tggtccagct atggcatctg cggatatatt tgaaataaca gtagaaggaa aagcaactca
     9061 tggagcaagc cctgaatttg gagtggaccc aataaacata atgtcgcata tacaaatagc
     9121 attaaacact atactttcaa gagaaaaacc tcagaaagaa cctgtagttt taactattgg
     9181 tatgtttaat gcaggaaacg taccaaatgc tataccaaat aaagcatcta tgtcaggaac
     9241 tttaagaaca tttgaccaat ttgttagaga aaaattaaaa aatagaatta ttgaaatatc
     9301 tacaggaata gctaaatcat ttggaggaaa tgctaaagtg gaatttaaaa tggggactcc
     9361 tgcagttata aatgatttgg atgtaggaga tgagattgta tcttatgtta aagaagttat
     9421 aggaaatgaa aatgtaatag actttccatc tgcaatgggt tcagaagatt tttcagagat
     9481 tttactaagg gttccaggag tatttttatg gattggaatg ggaagtatta gtcagggata
     9541 taattttggt atgcataatc ctaaagcaat ttttaatgaa gagggattag tttatggttc
     9601 agctttattt gctcattgtg cagtaaaatg gttagaaaat aattttagta gataataaca
     9661 taaaatatta ttaaatatca taaaaggtat atcttaaaaa gatgcaaaga tatacctttt
     9721 agaaagtcat atataatttt acttaatatt agatgataaa atatttttat gcattttgga
     9781 ttttaatatt tattttctga tataaataat ttgtataccc tttcaggtct accttttgtt
     9841 gagttttgtt tttcatataa tatagtagcg tttccagact ttaagagact acttagaaat
     9901 ctattagcag ttctaactgt tacatttaaa catgaagata attccttagg agtaacttca
     9961 tctctttgta gaatttcagt aacagatata atcttttgaa tagtaagagt agacaattta
    10021 gaacttttgg caattgtatt tatatgtgga gttactttat ttgaaacagt aagtaactta
    10081 tcacacagta aaggtcctat tgtctcataa ttttcgttaa ctaagaaact gtaatttaaa
    10141 ggatttattt tagattcctt attagcactt atggcactag acatagcttg ctttatatca
    10201 ttaccaattc catatccaac acatacttta atatttgtac tattttcaat agattctttt
    10261 atataacatg cagtaaaatt tttagttaat gatttaatcg tttcacattt agtgaaaatt
    10321 ttaaaagaat tactattttt ttcaattata aaatcggtta atttatcttt tttaatacta
    10381 tttaatattt tataaacttg gtctagagta gagtcatcat aatttgtatc tttaactgtt
    10441 atatatatta cacttggttg atttcccttc attctatgta aatgaatatc ttttattaag
    10501 ttattaaaag tattttttaa taaatctata tcagggtata tgaaatgaaa gtttactcct
    10561 gcattttgta acataggaac aatactacta aaacgagtag tagaaaaatc gattttccct
    10621 tcattccata gattaatatg tttttttgca atcttttttt caagttttgc tatgtcagat
    10681 aaactcattt tagatgcatt ttcatcaata ctatccatta agtctccaaa tgtgccattt
    10741 gataaataat atgatatatc ttttggatta tctgcccagt ctgttaagtc aaagtatgct
    10801 ctggaaaaat ctaatgtatt atatttaaag ataagcttta gaaacatatc atagtaactt
    10861 tgtaagtcag taccaaaatc aactagaggt ttttttatag ttccagcctt ttttgtaata
    10921 gcttttttgg ggatagggcc actaactgca aatccatcaa acctatcttc tatttctaaa
    10981 tataaatctc caacatgaga aaaatctcta tagatatata ttttaatttc tgcattaata
    11041 tttaactttt ttatagaatt atctacaaac tcttttaaaa attcggttgt tatgactgct
    11101 attttcatca tagattactt ccttttcaat tattataatt attttatatt aaatattaca
    11161 ttaatctatt gttctagtta aaatcgtatt tcaattaaat tcaattaaaa taaataactc
    11221 tttataaaaa gcaattattt aggtagagga atatttgcta actttaatag tctttgttaa
    11281 ctttaatttt atttaagtta ttatagtctt actaatttta taattgtaga cttaactact
    11341 ttatgttctt ttattgttta tgtagttgat ttagaagtta ttttaaccat ttattttaat
    11401 tgaagttgtt tgatttttaa atcctttgtg taatctttgt ttttctagcc atgataaaag
    11461 tatttctatt tgtatcacta tatataataa tatcatgaca aaatatttat tgtctaacta
    11521 attataatct atccaaaatg aaaatgggtg tgagatatta ttacaacaaa ttgtattagt
    11581 attaaaaaga ttaaaaaatt aaaaaactaa acagttaaat aattaaacga ttaagtgact
    11641 gtcatataac aatttataat tttatttaac agccacttag gtatttaaga gcgatagaat
    11701 tgagtgtata tcaaattata ataatttcct agtaaataat tgaaaaataa ggtagtccat
    11761 ttttatattt gatatctggt ttaccttgaa taagtggata tagataattt agggcttcat
    11821 cagttatata attacattct tcatttatcc actctttagg aaaatatttt gtcttgttag
    11881 ctattttaga aatatcagta tgactaactt catacttata aggattattg catgttctat
    11941 taatgatagc cataactcct gtatatcctt ttttagatag ttctatccca tatgcaccaa
    12001 cttcagctgc ttgttttaaa tcagtatatg aagctaaatg catagcacat ctttgtatta
    12061 caccaagttc taaggttttt actcttgttg taattgatga tgatactata agattcttta
    12121 gatagttgct tactccacct aattgggaat gaccaaattt atcatgagca gaagtttttt
    12181 gttcgcatat aaagttgccg tatttatccc ttataccttc tgatgcaact atataagcct
    12241 tatttttttc tttaaatatt tttctaacat cttctataaa cttatcttca tagaaatcaa
    12301 tttctggaag gtatattaaa tcaacaagtg gagtatcatt aagtctagct agtgaagaag
    12361 aagctgctaa ccatccagta tctctaccca tagtttctac tataaatata ccattatttt
    12421 tgtatataga aaaatctaag tatgtttcca ttattagtgt agaaataagc tttgcagcag
    12481 aaccaaatcc aggacagttg tcagttccta ataaatcatt atctattgtt ttaggaatac
    12541 ctataaattt tattttacaa gtagtgtttt tgcagtattg agaaaatttc atcactgtgt
    12601 ccatagaatc atttccgcct atataaaata atgtatctat ttcatattca tctagtatat
    12661 ttataaccct atcataatca taagaattta ttttataatc agccagtttg tatctacaag
    12721 aaccaagacc agacgaaggt gtttgcttta aatttaatac attatctttg tttaaggagt
    12781 ctgaatctat tatatttttg tttaatatac cttcaatccc attgatacca aaatatactt
    12841 tatcaaagta tttagtttct aaattttttt cataaatacc aactgcagaa gcattgataa
    12901 cagctgtagg gcctccagat tgggcaataa tacagtttct cattttacat ctccctttca
    12961 ttaatattgt tatgaatggt actttttatc gagtaaattt atatttatag tctgattata
    13021 tattaaatga actagctata tcaattgagt tagtatagac aataaatgcc ataagaatgt
    13081 ggtaaaaaac taacttttta tatacaatta atgtctataa tttaccacaa tataaaggta
    13141 cttttaagga gtagtcaaag tgttgtgaaa aaaaatatat cagtgtaata ttaatttaca
    13201 aagcaaaata agttcacagt taacaaaagc agaataagtt cataaaagaa atatatacag
    13261 caataaaagt aatgctaaat aaaagtaata ctggattaaa aaatattaaa aaaatatcaa
    13321 aaactaatat ttaatactaa ttaaagtaaa atatggaggt atacaaatat gaaacacata
    13381 aaaatagcag caggattagc acatgtaaat tacggtcata tatcagccgt agttaaagaa
    13441 gcaatggatg caggtgtaga ctacgtacat tcagatgcag cagatatgca tgacttaaaa
    13501 aatatgcaac ttatgggtgg tcatcaaata atagaagcaa taagaccagt gacagataaa
    13561 ccaatagaat gccatatata cacaagagat tgtgatagat tatttataga aaaaatagca
    13621 gcagcaggat gtaacttgtt gataatacca gcagaacatt ttttaggtgc tcctcttgca
    13681 tacataataa attactgtag agaatttggt atgaaagtag gattaacaat tggatgttac
    13741 acaccattat gttttgttga tgaagctata tatgatatag atagattgca aatagtcgtt
    13801 catggtgttg atgaaactga tggaaaagat aactggggat ggagaaagag tgctgttgat
    13861 ttagtaaaaa gagctagaaa aatgatagac gaaaagaatc ctaagtgtga attagcaata
    13921 gatggtggac taagagcaga caatatggag ccattaatag aatgtaatcc agacgttgta
    13981 atattatctt cagcaatatt caaggataaa gatggtataa cagcaggtgt taagaattgt
    14041 agaaaagcta tagatgaagc agctacaaag tttggtttag aataagataa taaactatga
    14101 taaaagagat gctttcttaa tgttttaagg aagcattaat tttatgaaaa actacaggaa
    14161 gctagactaa tttaattgat tttttatact gaaacaatat aatcatagga ggttattaaa
    14221 tgaaaaaaga aatgataaaa gattatttac tgataacaat atcagcattt ttaatggcat
    14281 tagcagttaa ttttttcttt gcagaacata cattagctcc aggagggata acaggattat
    14341 cagtcgtact tagcacattc ttatcaatgc ctgttgagaa tatatcatta ggaatatcta
    14401 tgccactttt agtaatggga ttattatttt tagggaaagg ttttggaata aaaacattat
    14461 atataactct tatgggccca ttattcttaa aagtaatacc acaaactcac ataactgaca
    14521 atctattagt agcaggagtg ataggtgggt tattggtagg aactgctata gggatagcaa
    14581 taataagggg ctgtagtact ggtggaactg atttagttgc aatgcttatt aataaggtaa
    14641 ttaagtttct gaagttgcca gttatattat ttatgctaga tggatttata atagtagcgt
    14701 ctggaataat aagtaagaat tttatgatat ctatatattc attgatatct ttattactca
    14761 taataaaaac aataggattt gtaactacaa agtttggtaa gaatactgaa gaaattaata
    14821 atataaataa tgcttgtgct caataaaaat aataaaattt actttaaata taaaagttta
    14881 aaaaatataa aagcttaaat aaaaagatta aatataaaaa atgaaggtgt ctcaaaatga
    14941 attttttagt tcatgaggca ctttttctat gaaatcaaat atatttttat catttggaca
    15001 atgaaaaata ggtttatctc tatgttgaga caagcctatt tttgtttatt tataagataa
    15061 tattttattt ttttcagaat atagtttttt tataattgta ctaagtctag aaatatcatt
    15121 cttacttaaa agagcattta tatttatcag ttcagcatta tgttcaaagt ctacaggagc
    15181 agtagttagt attaagtcca catcatcaaa gttcgtaaat gaaatagaag atagaggtat
    15241 agatttaact atattaatct gattaaattc aaaacttaac ttttggacta ataaattaga
    15301 tacaccatca ctacagtgac atattaaaat agtatttagg ggagatttgc ttctatctat
    15361 tgctgatatt aaatataaag caagatatcc tatttcgtca tcattgactt cataattcat
    15421 caactgtttg ataacagtat tagactcttt agcagcctta taagtataag gaaatgttct
    15481 ctttatatca tttaatatgg gatttgtaat agtaaaccca tagtttattc tgtgtagaac
    15541 tgatttaagg tgattagcaa gagactttat taatattaaa tcatccttta agtttaagtt
    15601 tagtatactt ccccaatgaa cagctattgc ttcagataga ttatactctg taatttcttc
    15661 atttttaaga ggttcaagag tagtagtttt ctttattgat ttaagtaatc cttctatatg
    15721 aagaaggata taacagacct cttcatcaat aaattctacg tcaaaagctt cacttaggtt
    15781 agcacataaa atttttgatt tttcaaagta ttcatgttct aataattctc taaaaaaacc
    15841 ttcagataag ttaatatatc ttttaattga aattctttta atacatatag ctatatgtat
    15901 taagaagttt atataatact catcactaag gttacttttt tctacttgta aggtgtgtag
    15961 agtgatttga gataatttct caaaatcata gttaaatagt tccttaaata tttttgttga
    16021 tggggtatct ttacattcat tagatttcga gaataatatt tctttaagtt cagtataact
    16081 tttattaacc gtagcaagtt caaaaattgc ttttcgaata tctttctctt ttccctctaa
    16141 atatactcca ttattggttt ttctaactag agtcacttta aagttttgca aaaaattttg
    16201 aataactacc aagtctttat gcacagtagc tctactaaca tgtagttcat tgcaaatatc
    16261 acgaatccta aacttagaat ttttaataaa taatttactt agaatatacc tttttctatc
    16321 ttcaggagag tatgcatata tacaatttga tttatcattg atatctctaa taactttaag
    16381 ttttatatct tcattaccta gaataataac ccctgaacca gtttttttat ctagggataa
    16441 attaaactct aaaagccatt catctaaaat aacaaggtca ttacgaatag ttttattact
    16501 aacttttaat tcttcggcaa tagtatctac agtaatatag tctgagctgt tgagtaataa
    16561 ttcagcaata tctaataatc ttttgttcaa tgcaaa
//
LOCUS       DAENCV010000081        16575 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAENCV010000081 DAENCV010000000
VERSION     DAENCV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.082"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            118..618
                     /locus_tag="KQD82_002616"
     CDS             118..618
                     /locus_tag="KQD82_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381078.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(868..1647)
                     /locus_tag="KQD82_002617"
     CDS             complement(868..1647)
                     /locus_tag="KQD82_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381079.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1644..2123)
                     /locus_tag="KQD82_002618"
     CDS             complement(1644..2123)
                     /locus_tag="KQD82_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4381080.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAINNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2932..3633
                     /locus_tag="KQD82_002619"
     CDS             2932..3633
                     /locus_tag="KQD82_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG4381081.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            3659..4315
                     /locus_tag="KQD82_002620"
     CDS             3659..4315
                     /locus_tag="KQD82_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381082.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            4420..6060
                     /locus_tag="KQD82_002621"
     CDS             4420..6060
                     /locus_tag="KQD82_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG4381083.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6418..7740
                     /locus_tag="KQD82_002622"
     CDS             6418..7740
                     /locus_tag="KQD82_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381084.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIGTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7937..8944
                     /locus_tag="KQD82_002623"
     CDS             7937..8944
                     /locus_tag="KQD82_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG4381085.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            8982..10394
                     /locus_tag="KQD82_002624"
     CDS             8982..10394
                     /locus_tag="KQD82_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG4381086.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIL
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKGMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            10536..11801
                     /locus_tag="KQD82_002625"
     CDS             10536..11801
                     /locus_tag="KQD82_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4381087.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11843..13441
                     /locus_tag="KQD82_002626"
     CDS             11843..13441
                     /locus_tag="KQD82_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG4381088.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            13476..14498
                     /locus_tag="KQD82_002627"
     CDS             13476..14498
                     /locus_tag="KQD82_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG4381089.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(14981..16384)
                     /locus_tag="KQD82_002628"
     CDS             complement(14981..16384)
                     /locus_tag="KQD82_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381090.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT         5844 a         1985 c         2984 g         5762 t
ORIGIN      
        1 tatagaaata tacaaagata ttttaagcta atatatagta atttatatag cattataaaa
       61 tagaatcata taaaataagt taactacaat caaaaattaa aaaggggaaa tgttaagttg
      121 atagaaggca taaaatttat aaaggcagaa gaaaagtata tttattcgta ttggcaaact
      181 tttgataaaa tagcaaaaga gaggaaatat ctagctatgg atgaagcctt tccttttgaa
      241 gaaacagttg aatttataaa aaatattata aataaaaact taccacaact gtttataatt
      301 gatttagaat cagataactg tattggttgg tgtgacgttt tacctaaaac tgaaaaagtt
      361 ggttatctag gcatgggtat tcttaaagag tatagggaaa aaggtatcgg aagtagtctt
      421 ttaaaacaaa ttattgattt atcaaaagaa tatgggtatg aaaaaattga gcttgatgta
      481 tttaaaagta atagtagagc tattcatgtt tataaatctt tagggtttgt agaggtaaat
      541 acaatatcta gtggttttac atggaatgat agacctgtaa aagaagaagt aattcaaatg
      601 gagcttacac ttatataaaa taaagttaaa gtctttaaaa ttccatatac acttgtataa
      661 aataaagtta aagcccttaa aattccatat aaaagaaata tttttaaggg ctttttaata
      721 atggttcttt tgaaggtagc taaatatcta aaattttata tattaaattt aaagaaaata
      781 taaagatgga agatacaacc atgtttacta ataaaaaaat taaagtcata aataataaac
      841 tcataaacaa cttattatta cttatgttta ttttgcatct attacttcat gagccatttt
      901 aactaagtca ttcatagata aatttgagtc ttgaactgta aaagaatagt aaactccatc
      961 ttgtttccat gtcaaaaact taaatttatt aacttcaact ttgtctgatt cattatttgg
     1021 ccccacacta aatgtgtatt tgcctgattt ttcatcaatt ttatcctgtt ctgtcatttt
     1081 ataaccttct ggtacaaact tttgtgcgaa tgcttcataa gaaatattaa tatctttgta
     1141 agtatctaca acttttagat tgtcagaact cttcccaagt acatcatttg ctgtgtcaag
     1201 agatatttca tcattgccat ttttatattc aaaactaagt gcttttgatt ttcctaaaac
     1261 attattttct ttgtcaaatg cttttttatt tacagtatat gctcctttaa atttatatcc
     1321 attactaaat tcagaaacta tattaggggt aaagttaaag tcttttttta cttgttctgt
     1381 agttgggata gatgtatagg tagccttagt acttgatttt ccaactaaac tatatatatg
     1441 tcctactgca agggctgttg tgctcaatat caaagtaatg gctaaagttg caactaagcc
     1501 ttttctaaat ggtaatttgt gtgttttatt attaatcatt ttataatttt cctctctttc
     1561 tggtaaacta tttagtgttt tgcttaaact attatgaaaa ctttctggaa cttctggaaa
     1621 cgaattattt aatatatttt tattcatatg attacctccg tgttttctaa tttagttttt
     1681 aataatcttc ttcctcttga taatctactt tttacagttc cttgaggtat atctaatatt
     1741 tccttaactt catctactga ataaccttca atatagtaaa ggactattgg aattttaatt
     1801 tttttaggta gactttgaat agcagtatac aaatcgctat agtcatcttt ttcatcaatt
     1861 tttttgtttt tgaagacatc ttcaaatgat agagatttta atcgtttgcg tcttaatgaa
     1921 ttacattcat ttatgactat tctaatcagc catgttttaa agtattgttc ttccttaaga
     1981 gaatccaact tttcatatgc ttttaaaata gcattgttga tggcatcttc acagtcttgg
     2041 tcgtttccta gaatagactt agatactcga tacaaagtct gttctgattc aagtatattg
     2101 tttgtaaatg tagttttatc caactaagtt ccttcccttc tatgcaaatc gtagcgctac
     2161 ttttttgcct ttcacttatt agataaaatt ttactataga tggttcactt tttttatgat
     2221 tatttgataa atttaagttt gaaagttttg cctattctat acaaatattc tataatatat
     2281 ttttttctag tattttcccc tcatatataa agccatgtag atatattaaa ctttagaagc
     2341 catttatata tttagtttta tagtaagtgt ttatattgag taaattagaa aatagtaagt
     2401 atttaatgtt gagtagttag aataacaatg cttttggatt atttacttca tgtacagatt
     2461 taaaataagt tttttaaata atgagtaaag ataacagtat cttgactaaa agtaggcttt
     2521 taagaaaaaa caatgaaact taaatgagaa ttaagagtta gaaatgtcat tattttagat
     2581 agagatgaac ataaataaac ttaaaaaata taaattataa aaataataat tttttttaaa
     2641 ataataaagt tttttgatta aaagttttat attgaaatgt caaaataata aaagacctta
     2701 taagttaatt acaggttaaa aaaataatat gtgaattttc tgatatttac taattatgaa
     2761 taaaatattt aagtattaat gtttttagtg agactttaag acagataatc tcctattaaa
     2821 gatactatat cttagataaa ataaattaaa acaatataaa aacataacaa gattacagaa
     2881 agaaagctca caataaaatg ctgaaaaatt tattttaagg aggaataaag tatgaagttt
     2941 caagaaatga ttaatagccc aggtctttgg attgtttcta gttttttagt aattatttca
     3001 gttgttcaag caatcgtatt tatgaaagag gcactaaaag aggcttcaaa tcttggtatt
     3061 gaaaggaaag gaattaaagc agcaattcgc tcagctagtg ttacagccat tggaccttct
     3121 ctatctcctg ttattacatt attatcactg gtagctgtga ttggcgctcc tacgacttgg
     3181 atgaggctct gtgatgtcgg agcagctaga acagagcttg gagtaatatc tcttacatcc
     3241 aatttatctg gggtagaagt aggtagtgct gcatttggag cagaagcttt ttcgtatgca
     3301 ctatggggga tggctttaaa taacttaggt tggctatttg ttgtatttat tttgggtcat
     3361 agaatgcgtg gaattgttga aaagatgaac atgaagtata atcctacttg ggttaaaaaa
     3421 ctacttgcag gagctacagt tggattattt gcttaccttc tttcaaatca aataaaaaca
     3481 tttgaaatac caaaacttac accagcaatt atttcagcta tagttatgct agtattgaca
     3541 actttattta aaaagaatca acgattacaa gaattatcct taggtattgc catgttaatt
     3601 ggtatgtttg gtacacagat tatattatcg tgattaaaac ggataagagg tgataattat
     3661 gtatgataaa aagatgataa aaatagggag aatttctata ggtcttgcaa ttattgcaaa
     3721 ctttattccc gctatctatg taggtattcg ctttggtcag atgccaatgc tttctgttat
     3781 atttcaaatt tgggggttag tcgctgcgac ttatggggtt agttggattg ttcaacctat
     3841 agcttattat cctacactag gagctccagg ttcttatata ggttggcttg caggttctgt
     3901 tggagatata cgtatgcctg cagcatcaat ggcacagaaa attgcaggtg tagaagcagg
     3961 aactcatgag ggtgaagttg ttggaacaat tggtacatgt tgttcaatct ttgtatcagc
     4021 ttctatgatt accattttta ccattgtagg ctataaagta attcctttgt tacctgaatt
     4081 tgttactaat tcatttaatt atatacttcc agcattattt ggagcaattt atgttgatat
     4141 tgcacgtaag gatattcgag ctggtggatg tacgattgct gtagcgttag caattatgta
     4201 ctttggtgaa aaaattggta taccaggtgg attattaaca ttgtttattg tgttaggtgg
     4261 gatacttatc acaagggtct tctttgtaat tgataacaat aaaacaaaag aataggattg
     4321 cttattaagg agaaagacta gaattatttg ttgagaaaaa agaataggat tgtttattaa
     4381 gaaatatgaa tataaataag tcttgaaagg agaaaaagca tgtttgatgt taaaattgtc
     4441 aatggaataa tcgttgatgg tacaggaaat tcaagatata aaagtgatgt agggattgta
     4501 ggagacaaaa tcatggctat tggagattta agtcaaaaag aggctaaaga gactatagac
     4561 gcaactggaa aatttgtatc acctggtttt atagatttcc atactcattc tgatttatct
     4621 cttgtgtatg ataaatacac aagaagccgt atccatacag gtgtaacaac agatgttatt
     4681 gcaaactgtg gtataggagt tgctccgata agagaagaga agaagcaaga gttaattgat
     4741 tatcttggaa ctaggataat tggtacaatt ccaacaaaat tagagcttca ttggaataca
     4801 atgcaagaat actttgatta cttaactgaa aattctccag cagtcaatgt tgtagcttat
     4861 gtagcacaag gacctgtacg tatcaatgaa atgggatttt caaaggaacc agctacttta
     4921 gagcaactta aaaatatgaa gttggaagtt agaaaagcta tggaagctgg atgtgtagct
     4981 ctttcatcag gattagttta tttaccagga gcatacacaa agaaagaaga aatggctgaa
     5041 ttatgtaagg aattgatacc atacaatgga tactatatat ctcatattag agatgaaggt
     5101 gatgaagaaa tggaagctct tgatgaagca atttatatag ctaagactgc aggagttcct
     5161 ctacatgttt ctcatttaaa agttatgggt cataagaatt ttggaactat tgatgaggta
     5221 tttaaaaagc tagatgaagc tgaggcagat ggtttggaag taacttttga ttgttaccca
     5281 tatacagctg gaatgacatc tttgggtgca cttttacctc cttgggcttt tgaaggcggt
     5341 gtagaaaata tggtaaaacg attagaagta caagaaaata gagacagaat tataaaagag
     5401 ttggaggaag ggattcctgg atggcaatgt ttctaccaat tagcaggtgg gtggaacggt
     5461 gttgtactag cctctgtaat gactgaagca aataaatatg tagagggtaa aactttaatg
     5521 gaggtagcca aaatcaatgg tgaaaatcca tttgatacgt tctttaggct tttgattgaa
     5581 gaaaaaagta aaattcaagt agttgttcat acacaaggtc aagaggatac agataaggtt
     5641 gtttgccatc caaagtcatg tataggttct gatagtatgg acctttctac agaaggttta
     5701 ctttctcttg gaaagcctca tcctcgtgca tttggtactt ttggacgaat tttctcttac
     5761 tatgttagag aaaaaggtat gttgactttt gaggaagcag ttaaaaagat aacttatctt
     5821 tcagcaaaac gcttaggcat ttataaagaa aggggtctac ttaaagaaaa ttattttgct
     5881 gacattgtgg tatttgaccc agatacaata gaagataaag cgacttatag taatcctaaa
     5941 cagtatacag tgggagtgga atatgtgttg gtgaatggta aaattgcatt ggcaggagga
     6001 aagcaaactg atgtatgtgc tggacgtgta attaaaaatc ctcttagcat agcaaaatag
     6061 aatacattaa gaacttttat aaaaatagtc tttctttgaa tattaaggaa ggctattttt
     6121 gttaaattaa taaagattac tctacttgtg ttttctaaag aatttaactt taagtagaaa
     6181 aaacaatata ttaattgtaa aatgtaatta atattaatga ataagattta aatacaaaaa
     6241 tttaaataca aaaatgtttc aacagatagt cgtaattaaa aaacttaaag taacttttta
     6301 tattaggtat aattgattat tcaaaattct atgaatcagg agtcctgtgc ctttagtcat
     6361 ggtaggttta gaaaatgttt taagagataa tagtatcatt aggaaaggag agacgaaatg
     6421 aaaatacttt atgtaggacc aggtatggta atcgataaaa tactagcagt aagtaaaaag
     6481 aattttccta atattgaagc agattttctt agatatgata gatatattga agtacctgat
     6541 ttattggaaa aatatagagg tagagtggat gcagttcttt ttacaggtaa gtcgccattt
     6601 aaattttttg aaaaaagaca aaaagatttt gtattgtctg attatattcc tagacatgaa
     6661 acaacattgt acagagtgtt gcttgaaatt acatatttgc ttaaattcga tattagtaga
     6721 cttagtatag gtacttatga taaggctatg atgaagagat tgtataaaga aattggagtt
     6781 gaatttaagg atgaacatat gttttttgca gagcaaaagt atttagaaga ggactatata
     6841 aagtttgtgc ttgaatttca taaatataat tatcaaaata atgatgtatg ttgttgtatt
     6901 acaggtcttg aagaggccta tcatataatg aaaaaagata atattccagt agttttagcc
     6961 ttgccttctg aggacgtaat ggtgcaaagt ataaaaaaca ttcagatgcg ttatattgca
     7021 aagaaaaaca atgagaatca gattattgtt cttgctatta aactgaacat gccttcagaa
     7081 tattcactaa tgaaggaaga tgagtacgct tatctttcac aaaggattaa gattcttgat
     7141 aaactatacc atttcaatag ccgtatagat ggtgttttgg tagagcagag tagaagtgaa
     7201 tttatgattt ttacaactaa aaaaatactt gagttagaaa caaagaaata cagagatatt
     7261 tatttgttgg atatgattcg tgaagtttca gttataaaca cttatatagg aattggttat
     7321 ggaaaaacag caaatgaagc taaatttaac gcttatgaga gtattaagat ggctcaaagt
     7381 caaaaaaatg atgcagcata tgtagttttt gaaaatggag aaattatggg accactaaag
     7441 ccaaatccta ctgtcaaaga gaaaaatagt tttgatgaac gtttttatca aattgctaca
     7501 gagacagggc taagtgctaa tactgtatat aaagtattca ataaaataat aaatagtgga
     7561 aaaactgaat ttacatcaaa ggaattagct actatatgtg gtgtaagtat tagaactatg
     7621 gacaggatta tacttagatt gtgtgattct ggttattgtg aggtagtgag tgaacagctt
     7681 atgagcaagt atggtagacc cagtagaata ttacgtttta aaccttttct gttgttttaa
     7741 atagatataa gtcaaaaatt aaatttttat gataacattt tctttacaac tttttagata
     7801 tatgttaata tttagaaaaa tgcatatgta ttcataaaat aaactcaaaa cagctaaaaa
     7861 atcattaaaa aggaaatagt atgcataaaa tgagaaagtt aatttataac ttaaataaat
     7921 ctgggaggag ataattatgg gaaaatatca ggattcaaaa aaagttgtaa gaaattattt
     7981 tgaagcatta gaaaatgcta ctggggatga ggttgaaaat gtcttaaagc aacatatgaa
     8041 atcagattat aactggaaag gtgtgtatcc atttcgtgaa caagaaggca ctgaaaatgt
     8101 agcagatgta ttttggaagc ctttaaaaaa atcattgagt aatatgcaaa gacgacaaga
     8161 tattttcatt gcaggaacaa atgagattga caatagtgaa tgggtaatga gtatggggca
     8221 ttttatggga ctttttgata aggattggct tggcattaaa catactcgca aaatgattag
     8281 tttaagatat gcagaattta actgtgttca agatggtaaa attgctaaaa ctggattatt
     8341 tgtagatata attgggttta tgattcaagc aggagttaat ccattgccac cacaaacagg
     8401 ttcatatttt gtatatccag ggccaattga ccataatgga ttactttttg aagatgcaga
     8461 tgaagttgaa ggagtaaaaa ctttagcact agtaaataaa atggtagatg atttatctga
     8521 attaaatgaa agtggtgcta tgggatgccc accagaaata cttgaaaaat cttgggctaa
     8581 agatatgatt tggtatggtc caggtggaat tggtgcttct tatactatac ctagatatca
     8641 agagcaacac cagctaccat ttagaaataa tttaaaagga aagactttta atgggcatgt
     8701 atgtagattt gcagaaggta attttgcttg tttctttgga tggcctaatc tgactaatgt
     8761 accatgtgga ggttttttag gattaccagg tggagaggtt aaagcagata tgcaagttgt
     8821 tgatgtttat tatcgtaagg gcgataaact tcaagaaaac tgggtgttaa ttgatattcc
     8881 ttattggttg aaacaacaag ggcttgatat tcttgagaga actaaaagta tttttaatgc
     8941 ttagttatgc atacgtattc taaaacaaag ggggatataa agtggcatct accaacaaaa
     9001 ataatgcaac aagtaaagag gaagaaaaag aagtttctac aagtgggctt gaatcatgta
     9061 cggttaaaga aaacaaagct aaaatgaatc aaactaattt tagtaatata aatgaaaatc
     9121 aaacaaatga gaataaaacg aacacatata aaggaaatat agataaaggg aatatagata
     9181 aaacaaacac aaataaatcc gacataaata aagttaacat agatacagtg gatacagata
     9241 aattaaatac aaatactctt actaaaaaaa ataaaaaagt tatatttaag ggggaagata
     9301 atatgtcatc tacaaaaatt tactatggag attcattaga agtaacgcct gttggagtaa
     9361 tggattacaa tgatttttca aaacagacaa aaagggaaca ggaaatacct ggatttgatt
     9421 ctaagtatag agattttgta gattatatta tgaagattac acataatatc tgggaagaaa
     9481 aaggtatagg tgttatttat gatacatatc acaataatgt tacaatgcat tgtggctctt
     9541 caaatttagt tggtattaaa gatgttattt ccaatactct tcaaacatta catgcgtttc
     9601 ctgatagaag attaattggt caaaatgtaa tattgtctaa ctttggtgct gatggttttt
     9661 tatcttctca ccgtgttttg tctacagcaa ctaacttagg tgatagtaat tttggaccag
     9721 caacaggtag aaaaataaac tttagaactg taattgattg tgccacaact aacaatagaa
     9781 tccacgaaga atggttggtt agagataatt tatggattgt tactcaatta ggattaaacc
     9841 ctcaggaagt agcaaagggt atggcaaagg caagtgaatc taaagtgctt agtttacaat
     9901 caacttatgg tatctgtgaa tctatggatg gtcaatttat gccaactaag tatcaagcaa
     9961 aagatgattc tgttggcgaa atgatgcttg aaatgacaag ccgcatttat aattataagt
    10021 atataaatga agttaaaaaa tactatcatg ataatgcagt agtacatttt atatgtgata
    10081 aagatttaaa tggctatgat gagattcaag gaatgattgt aagtttactt gcatcaattc
    10141 caaatggaag ttatgaagtg gaaagagtaa cttgtaatca aagagaaaaa aatgaaggtt
    10201 atgatgtatc tgtaagatgg agattacgtg gaataaatga aggtatagga ttttttggac
    10261 agccttctgg aaagcatata gaagtaatgg gaatcaatca ttatcatata ctacaaggta
    10321 aagtaaagga ggagtggata acttttgatg gtatggatgt attaaaacaa atgtatatgg
    10381 gagtagaaga ataaagctgt ttagagtata aatagttttg aaatatatag ggaagtattg
    10441 ctttataaaa tttaagtggg gaatgtatat gaaaaataga tgtttaaact tttagcaaaa
    10501 ttatgactaa atttaaaagg gggaattata aaaatatgaa aacaacggtt gaaatagaaa
    10561 atgatatcat attaggaaaa gactattcaa acctaaaaag aggaattgca tttttttcaa
    10621 ttgctttaat atactttttt tattgctaca attttatggt gggtactttt attaaaccaa
    10681 caatgattta tgctttagca gatgggggat ttggtttttc attaaaacaa acagaagaaa
    10741 tatttgcagt tatgtctttt ggtacaattc caggtacatt tatttttggg gtgatttcta
    10801 caaaaattgg gaagaagagg actttaatta gcgttgcttt actgattgga ttgacaacat
    10861 ttataccaat gttatctcct acaaatatta tgttatggaa aatagcacgt ttgtgtacag
    10921 gagtagtgtt gggaggcgta tttggaacag ctatgccttt agttgcagat atgtttccaa
    10981 gtaaatatcg tggtaagtta gctgctatat taactgcttt attttcatta gcaatgattt
    11041 ttggtgggaa agtatatggc gttttaggtg atgcaaattg gcaaatattg atgtatactg
    11101 cgattatacc acctatagtt ggagctatct tagcaatctt ctttgtgcca gatgatttgg
    11161 agtatacaaa ggaattagtt aaaaagggaa aagaaactgg tgaaaagatt agctatataa
    11221 gtatgtataa gggaaaatac ttatggattg gattgggaac gattctttta tcaggtgcaa
    11281 attttacagc atattcagca ttttcaaata atgcaactac atacctagtg acaggaatag
    11341 gaatgtcagc agcagtggct gggtctatat atagtttaca aggaattggt caattagtag
    11401 gatatttatt ctggggttca attgcagata aatttggaag aaaaatacct tttataggta
    11461 tggcattagc agcagtgttt gtttttatat atacaaaatt aggtggagat aatacaacaa
    11521 cattctatat gatatcagta ttactaggtg ttgctgttgg atattctgga gcatggggag
    11581 catactacac agagttgttc ccaagtaaat atcgttcgtt gtcatctggt atgtcattta
    11641 atggaggaag gattatatct acgtttgcaa tacctgctat agctggtaca gcttctggta
    11701 ctttaggtat gatgactatc ttttacatat caatcgcagt ctttgtagca ggtgctattg
    11761 tatggttatt cttaccagag acgttaaata ataaaaaata aaatactagg aatttatttt
    11821 gttacttaga aaggggtggc ttatgatttc atcaattgat gtatgtatta taataggata
    11881 tttgctatta atgttagtta ttggttatta ttcaggaaaa gataataaaa atcaagaaga
    11941 ttatttcttg gcaggtaggt caatgccatg gattccaatt gctttatcag ttgcagctac
    12001 tatgattagt gcaaatggct ttataggtgg tcctggatgg gcatatgtag atggaatgta
    12061 tccagtaatg gttaatatta cagtaccttt agcaatattt tttgcactga gcattaccac
    12121 cccagttatc tataaattaa aaataacctc tatatatgaa tatatgggac ttcgtttagg
    12181 aaattattca agaggattaa ctattattca attttttatt aattcattga ttcaagctag
    12241 ttctatggta tatattccag tgcttattat tcaaatgatt actggctggt cactttatgc
    12301 tttagtccca atagttgtac ttgtatcaat tatatatact ctattaggtg ggattaaggc
    12361 agttatatgg actgatagta ttcaaatgat agtagtaata agtgctgtat ttatagtaat
    12421 tattgtagcc ttaaaaggaa tgaatctaag tttctttgat acattacaaa ttgcacaaca
    12481 gagtggaaaa ttaaatacat ttgatttcag tagggatatt tcagtgacaa atactttttg
    12541 ggctacttta attggtggaa ctgtaatgtg gataagatac ttttgttttg accaatctca
    12601 agtacaaaga gttttaacat ctaaatcttt aaaatgtgca aaaaattctt ttgtagttag
    12661 tgcatttgtt atgaatttag tttactactt tatgctattg gtaggagtaa ttttatttgt
    12721 tttttatggg ggaaggacat ttgaaacttc aaatgaaata atgattactt ttattttaaa
    12781 tgaacttcca gtaggagcca ttggaattat tattgcaggt gtgtttgcag cagcaatgtc
    12841 tagtatcgac tctatattaa acagtatgac aactgttttt acgaaggata tttatgagtt
    12901 ttatttcaaa aaagataaca aagaatcatc attaaaagtt acaatgatta taactgtagt
    12961 tataggagtt gttatgacag gttttattat tatgggattt ggaggaagca tcaaatcaat
    13021 tcttgacctt gttggtaact atatctcata ctttgctgga ccagctacag gtgcatttgt
    13081 actagcaatg tttacatata aagctaatga taaaggagta tctattggtt ttattgtagg
    13141 gttagtttta ggatttttta tttctaataa atataatgtg agttggttgt ggaatccagc
    13201 aataggtgct acaataacat tgatatttgg atatattttt agtgtcattt taaagaaaaa
    13261 tcataatgca gatgatatta aaaaatacac tgcatttggt atgagaaatc accttatttc
    13321 tcaaggaatg gataaagtag atggagtatc tatagtgcca ttttctttag gtaaacaaga
    13381 actaattgta ttagcttttt ttattatcca atatattata ttatttttga tacaatttta
    13441 agaagaaata ctagatattt aaggagaaat actaaatgaa tcaaaagaaa gttcattata
    13501 ataaatcggc tacctcaaaa gatgttgcaa gattggcagg agtatctcaa tcatctgttt
    13561 caagagcatt tggtagtgca aatggaaagg gcgtaaaacc tgaagtaaga gaaagaattt
    13621 ttcgtgtagc aaacgaaata ggatatgttc ctaatttggt agcacgggga atgattagtg
    13681 gaaaaactaa tgttatagga ttgatagttg gtgatagtct aggtccattt tacaatagaa
    13741 ttatcaattt atttgtagaa aaaattcaag agataggtaa acagtgtctc gtttttaaag
    13801 ttcctagaca acaaagtata gatagtataa tttcaaaagt aattcagttt caagtagaag
    13861 ctgtgattat tacagcttcg gccatgaata aggtgatggc tgagacttgt gaagaaaaca
    13921 atattcctgt gattcttttt aatcgtttta tacctggtat aaaaataagt acagtctatg
    13981 tagacccaat tgaaggtggg ggaatggctg ctgaatatct gcttaagaaa ggtcataaga
    14041 atataggata tattcaattt acgagagaaa caagtgaaga aatggaaaag aaaattggat
    14101 tttattctaa gttaagacaa agtgggattc ataatcttaa agaagaatct gcaaactatg
    14161 attatgattc tggatatgaa gcaggtaaga gaatgttagc attatcaaat cctccaacag
    14221 caattttttg cacaagtgac ttgatagcta ttggtgtaat ggatgctgca aggtttgagt
    14281 acaagttgag ggtgccagag gatttatcag tgataggata tgatgatatt caaatggctt
    14341 catggaagag ttataattta acgacgatta gacagccact agatttgttg attgaaaaaa
    14401 caattaacat acttaagtcc ttattaaatg aagaaaatcc agagtcagtc gttgaaatgt
    14461 taaaacctga attgattgaa agaggttcta cactttaagt ggtagatatt taattaagtg
    14521 atagatattt aattaagtgg tagatattta attaaatggt agatatttaa ttaagtggta
    14581 gatatttaat taagtggtag atatttaatt aagtagtaga tacctaatta agttgtagat
    14641 atctgataag aaatttttaa gttttcaatc gttctaaatt gtgagtataa aaatctacta
    14701 aatttagtta tgtaaaatat aaattatagg tgtagaaact aggctactag cttttatacc
    14761 acttcaaaca ttactggagg tagttaagtt tacagcagtt tagttttatg tagatataat
    14821 attctgtaaa tagacaaaat taaaatatgt aagtgtctca aaatgaaatt tttagtttat
    14881 gaggcacttt tttgtataaa atcaaatgta tttttataac tttaacaata aaaagaggct
    14941 tatctctatt ttgagacagc ctcaaatttt taaaactata ttagtttaca gcttcttttc
    15001 ctatcaaatc ttttaactct tcatgagtca tagtttcttt ttcaaataag aatttagaaa
    15061 cagaatgtaa atcttcaaga ttatccttta acatctctaa agtctcttta taacaaattt
    15121 caactatacg atttgcttcc ttttgaacct tatcacataa gaaagtctta tcatttccat
    15181 caatagtcat aaatcctaag ttactcatac catattcaca aaccatttgg ttagctattt
    15241 cagtgacttt ctttaagtcg tcctttgcac cagtagattt atgattaaat attaattctt
    15301 ctgaagcctt tccagcaagt aatattttta tttttccaat caactcatcc ttagtataaa
    15361 tatatctatc ttcatcaggt aattgaagaa cataacctaa tgcttgtcct ctaggaacta
    15421 tagatatttt ttgtatagga caaattccta ctatatcact tacaagagca tgtccagctt
    15481 catgatatgc tactattttt ttctcttttt caaccaatgc agagttttta gattctagcc
    15541 ctgcaataac tctttcaagt gccttgtcaa aatgttctga tgtaatacac tcactgtcat
    15601 ctctaactgc aaatatagct gcttcatttg ctatatttga aagatgagcc ccattgaaac
    15661 catgagtttt tttagctaaa agttctagat ttacagattt gtctataggt ttgtcatctg
    15721 tatgtacctt tagaatttca aatctagtgt gatagtttgg tgcacctata tgaatgtgtc
    15781 tgtcaaatct tcctggtcta agtaaagctt catctaataa gtcaagtcta ttggtagcac
    15841 ctattattaa tacattggaa tctttattaa atccatccat ttcaactaaa agttggttta
    15901 gtgtttggtc tttttcgttg ttactttcaa gatgtctctt tgctcctaca gcatctattt
    15961 cgtctataaa tattatactt ggagcttctt ttctagcttt ttcaaataga gttctaactc
    16021 tttttgctcc cacacctaca tatttttcaa cgaactctga accagtaaca ttaaaaaatg
    16081 atgagtttgt ttcaccagct acagcagatg caagtagggt tttacctgtt cctggaggac
    16141 cataaaataa tactccttta ggtatttttg cacccatttt ttgatatttt tgtggtgact
    16201 tcataaaatc gactatttca aatagttctt cttttacttc gtctaatcca gcaacgtctc
    16261 taaatgtagt ctttggtttt gtagaaagtg aatcatctgt ttgattatct ctttccattt
    16321 ttgtctgtgc aaagacagga gctggattgt tgattttctt aaaaaagttg tttagcattt
    16381 tcatattcac acctcaggta attaattatt gttaatcaat tttatatttt ccatatttac
    16441 tattttttat tatttatttt tcatggaaaa aatatacatg ttatctaaat aaaaataaat
    16501 ttgtaagatg tatgaaaaaa tgaatttatg tatatagaga tttctactta tgtattgaat
    16561 aaagtattac aaatt
//
LOCUS       DAENCV010000082        16232 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAENCV010000082 DAENCV010000000
VERSION     DAENCV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.083"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            44..694
                     /locus_tag="KQD82_002629"
     CDS             44..694
                     /locus_tag="KQD82_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG4381091.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(834..2210)
                     /locus_tag="KQD82_002630"
     CDS             complement(834..2210)
                     /locus_tag="KQD82_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381092.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2226..4139)
                     /locus_tag="KQD82_002631"
     CDS             complement(2226..4139)
                     /locus_tag="KQD82_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG4381093.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4275..4907
                     /locus_tag="KQD82_002632"
     CDS             4275..4907
                     /locus_tag="KQD82_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG4381094.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5046..5585
                     /locus_tag="KQD82_002633"
     CDS             5046..5585
                     /locus_tag="KQD82_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG4381095.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5761..5931)
                     /locus_tag="KQD82_002634"
     CDS             complement(5761..5931)
                     /locus_tag="KQD82_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381096.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6216..7283
                     /locus_tag="KQD82_002635"
     CDS             6216..7283
                     /locus_tag="KQD82_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG4381097.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7453..9354
                     /gene="cooS"
                     /locus_tag="KQD82_002636"
     CDS             7453..9354
                     /gene="cooS"
                     /locus_tag="KQD82_002636"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG4381098.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9347..9820
                     /locus_tag="KQD82_002637"
     CDS             9347..9820
                     /locus_tag="KQD82_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4381099.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRS"
     gene            9795..11021
                     /locus_tag="KQD82_002638"
     CDS             9795..11021
                     /locus_tag="KQD82_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG4381100.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11160..12347)
                     /locus_tag="KQD82_002639"
     CDS             complement(11160..12347)
                     /locus_tag="KQD82_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4381101.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12622..13446
                     /locus_tag="KQD82_002640"
     CDS             12622..13446
                     /locus_tag="KQD82_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4381102.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHREEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13575..14840
                     /gene="gluD"
                     /locus_tag="KQD82_002641"
     CDS             13575..14840
                     /gene="gluD"
                     /locus_tag="KQD82_002641"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG4381103.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15053..15166
                     /locus_tag="KQD82_002642"
     CDS             15053..15166
                     /locus_tag="KQD82_002642"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381104.1"
                     /translation="MHFKSKDFHLKYLVLFLKIVYWFNPFIYNGKIYGFGL"
     gene            15150..15641
                     /locus_tag="KQD82_002643"
     CDS             15150..15641
                     /locus_tag="KQD82_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG4381105.1"
                     /translation="MDLDCEFYCDERVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFV
                     AGLNKQSDIRKRVINMFSEKYKNGILMALIISLFSSITFFKFDSTSIIEENDFLERSR
                     YTFEGRQEDVFVTIDFIYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMD
                     LKK"
     gene            15971..>16232
                     /locus_tag="KQD82_002644"
     CDS             15971..>16232
                     /locus_tag="KQD82_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG4381106.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTIS"
BASE COUNT         6213 a         1935 c         2666 g         5418 t
ORIGIN      
        1 aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag cagaaaaaac
       61 aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg aatataaaaa
      121 gggtcaaaga tttgtagcat cagataaaga cttacaagaa gtgttcttta taatagaggg
      181 agttgcatta gtggagtgta ctactagagg aggaaataaa tttcttgttg acattgtacc
      241 tgaaaatgaa tttataggca aaatcagtta catatatgaa cataatttga aatgtgatat
      301 atttgcaaag actaatataa aattatttcg atttgaaaaa aatgtatttg aagagtttta
      361 tctcaaacca gattttattg cactatttca tagaaaatgt acaagaagaa tatatgaatt
      421 atataaaacc agaatggtaa gagaactatt ttcttgcaca gaagttattg cctattgtat
      481 atcaaataaa caagaaaata atgtctgcaa tataaaagct attcgtaatt tcagtgaaat
      541 ctattcaatt agtagaaaaa gtagatacca ttctctgcta aagttaactg aaaaggagat
      601 agtaaaaaaa atgggtaata catatgaaat tttaaactat gatgaattac ataaattggc
      661 atttgaagta aaagaatttt tggaagatga gtaaaaattg ttacttaatc aaaataaagt
      721 ataataattt ctgtaaaaaa tagaaatagt ctcactgaaa atcaaaaata ctttagttat
      781 tttcaatgag actgtttaga aatattgtat acttataatg tatagtataa ttattaatca
      841 atttccttta ctatattaat agcccatttt ttagataatg ctctaggaac accaaatata
      901 aacttaacca cttcttcact ataagtgagc aaaacgagta caaatatagg tgcaccttta
      961 aatgcagcta taaaagttag aggaatagaa gctaaccaca tacatcctaa ttctaaaaat
     1021 agagcatatt tagtatctcc tccacttctt aaaataccaa ttataaccaa tgtattaaaa
     1081 gatttaagag ccattagtat tcccattata aagaatatct tagttatatc agaagctaag
     1141 ctatcagaaa cactaaacat ttttaataat aatggtatat ttaaaattaa aataattcca
     1201 agtattaaac cagctgaaaa tacaagtata gaaaactttt tagcatattc aatggctctt
     1261 ttaatatggt cagctccaag ttcatttcca agcataatag atgcaccaga tgcaatacat
     1321 acagctgtca ttatgaagaa attacctgta ctggttgcta tctggctagc tgctatagca
     1381 gaagtaccag ctcttgcata tgcaacagaa tacaggacag ttccaattgc ccatacagag
     1441 tcatttacaa atataggaaa gctttttgat gaaaaagatt ttataaaaat tttatcaata
     1501 gcttttagat tttttagacc aaactttaat atatagtctt ttttataaag gtaaacataa
     1561 gtaatcatta aaattaattc acatattcta gcaataacag tagctagagc agcacctttt
     1621 acacccaatg caggaagacc gaagttacca aatataaatc cataatttaa aataacattt
     1681 gttacaagtg caaatgcact acaaatcata cctaacttag gatttctaac acctcttgaa
     1741 ccagtactaa atacagtact tacagcaatt aatggataac taaatactgc aatactaaag
     1801 taatctatac aaagttttac cacctcagaa tcatacgaaa atatgtgtat aattggcttt
     1861 ggtgttagta aagcaggaat aacaaataca aaacttaaaa caattgccaa tacacaagta
     1921 agacctgtga cttttcttat attactcaca tccttctttc cataaaactg tgaaataaaa
     1981 actccagcac ctcctgtgat accagataag gacatattaa aaagaaagaa aaattggttt
     2041 gcaactccaa ctgaagctac agatgtttcc cccaaactac ttatcataat tgtatctatt
     2101 acatttacag aagtagaaat taagttctgt atgataatag gtatacataa agatagaagt
     2161 attttataaa atcttttatt ttcgtctttt aaagaaaata gttcttgcaa ataatcctct
     2221 cctttttaca taaattcttc ccactcatca tatagagagg caaggttatt ttctaaatta
     2281 attttttctt ggctaacttc tttagcttta tctggattag agtatacttc ttcttgacat
     2341 aaaagtatat ctaatttttc aatttcaatc tctaactttt caatttcttt ttctatattt
     2401 tgtcttttta ttctattttt cttttctatt tctctttgtt ctctctcttt tcttttttca
     2461 tcttttattt gagttctagt tttttcttct ttttcttcta taatactcat ctcttggatt
     2521 tgcctttttt tatctatata ataatcataa tttcccagat attcagtaat tccattttca
     2581 tctaaaacta aaattttatc aacaacagta tttagaaagt atctatcatg tgatattgta
     2641 aatatagtcc catcataatt agttaatgcc tcttcaagga cttccttaga atcaatatct
     2701 aggtgattag taggctcatc taataacaat aagtttgcat ttgatagtat aagtttaagt
     2761 atggcaactc tagctctttc tccaccactc aatgtagaaa tcttcttaaa aacttcttca
     2821 tctacaaata aaaatgcccc taacatattt ctcaaagtag tttgagtaag atgagtatta
     2881 ttttcccaaa tctcatcaat tattgtgtta tctaaattaa gagttttttg ttcctgatgg
     2941 aagtaggaaa catgtacatt agttccaaat ttaatattgc cactaagagg ttgtagttca
     3001 ttagttatta ttttaaataa tgtagatttg ccaacaccat ttgcacctat aagtgcaact
     3061 ttttcccctc tatatatatc taaatccaag tctttaaaaa gtattctttc tccataaccc
     3121 atagatacat ctctaatttg aagaacatca ttaccactct ggacagaagg cgtaaattgt
     3181 atacgagctt ttttcctata agcttctggt ttatctaaaa cgtctacctt gtctaatgct
     3241 ttttctttac ttcttgcacg ctttaggtgt ttttctctac cataagcttt tagtctttca
     3301 attgattctt cttgcttttt tatttctttt tgttggtctt caaatttctt aagctctagt
     3361 tctttttcta ttttagataa ctcaacaaat ttagagtagt taccattata tactttaagt
     3421 tttttgttgt gaacttcaaa aattctattt acggattggt ctaagaaata tctatcatga
     3481 gatattaaca tgacagtccc tttatattgt tttaaaaata cttctaacca ttctatcgct
     3541 tcagagtcta agtggttagt aggttcatca agtaacaata gagttggttt cttaagaagt
     3601 aattttccta aaagaactct agttttttca ccaccagaaa gtatgcttat tggcttatcc
     3661 atatcattgt cactaaatcc taatcccttt agaactcctt tggcttcaga tttatatcca
     3721 tatccattta aatctgaaaa taattctagt ttatgtgaat attcatccat gattttttct
     3781 ataattggag agtttgtttt tgtactttct tcagatattt taacctctaa ttctcttaaa
     3841 taggattcca tttctatcaa atttttaaaa acttctagta cttcttccaa aatagtatta
     3901 tccgaataaa aattggtatt ttgttctaaa tatccgattt cacaatcttt agaagtgtaa
     3961 atatctccac tatcatatcc ataaattcct gaaattattt taaataaagt agtttttcca
     4021 gtaccattaa caccgattat accaatttta tcgccttcat ttactgtaaa actaatattt
     4081 tctaatatag aatctatacc aaaactttta tttaagttat tacatgacaa aacaatcata
     4141 aatttctacc tccttgaact atctaaaatt ataatgtttt gatataatat aatcaaggtt
     4201 atatgtatta gttttgaaaa agaaatattt tattgtgaat caaacaaaaa agaggacact
     4261 tttaggaggt gaagatgttg ggaaataaaa atatatcaat ggcagttata agaaggctcc
     4321 caaaatatca tagatatctt ggagacttat tagataggga tatacaaaga atatcttcta
     4381 aagaattgag tgatataata gggtttaccg cttctcaaat aagacaagat ttaaacaact
     4441 ttggtggatt tggacaacaa ggatatggtt ataatgtaga agctcttcat actgagatag
     4501 gtaaaattct tgggttggat cgaccataca acgcagttct tgtaggagca ggtaacttag
     4561 gacaagctat agccaattat gcaggattta gaaaagctgg attcgagata aaagctttat
     4621 ttgatgcaaa tcctagaatg ataggtttaa agataagaga gtttgaagta ttagattcag
     4681 atactttaga agactttata aaaaacaata atatagatat tgctgtatta tgtataccta
     4741 aaaatggagc acaagaagtt attaatagag ttgtaaaagc tggaatcaaa ggtgtatgga
     4801 attttgcacc tttagattta gaagttccga aaggtgttat agttgagaat gtaaacttaa
     4861 cagaaagttt atttacctta tcgtatttaa tgaaagaagg aaagtagaca acttaaacat
     4921 attaataatg agcagttgta aagagtattg agaaagtcag aagatttaat attaaatcct
     4981 ctggcttttt gaaatattag gataaaaatt taaataatgt atggaaataa acaatattaa
     5041 ggagagtggt ttttgtggag gaattatgga aaaaggcaat tgaatttcat ggtcatgaat
     5101 gtccagggtt agctatagga tttaaagcag cacttgcagc aaaaaaatat ttaggatgta
     5161 aacagtcagg agatgaggaa attgtatgta tttctgaaaa tgatgcttgt ggaatagatg
     5221 gtatacaagt agtgctaagt tgcacaatgg gtaagggaaa tttactactt aggatgactg
     5281 gaaaatcagt atatcatttc tttaatagag aaaatggaaa atctataagg atatatgtaa
     5341 agtcaaaacc atttgagatg gatagagaag gatacaaaag atatttatta gaaagtgatt
     5401 ttgaagaaat atttgagatt acagatacta agttaagact tcctgaaaaa gcaagaatat
     5461 ttaaatccct taaatgtgaa gtgtgtggtg aatttgcatc tgagcaatat ataaggattg
     5521 aagaaggaaa gaaagtatgt cttgattgtt ttgataaata tgaaagattt tatgaagagt
     5581 attaaaatta taaattagat taaagttaaa agtattagat atgtgctaac agtattttaa
     5641 gatgaattag gtagcaacat aataaagtat ataaataata ttaattgaat catacataat
     5701 taaaaagagg ctgattagta tttataaaat aaaatcagtc tctttatgaa taacgaatta
     5761 ttattctggt tgaggtgcat ctacaggaca agctcctgca caagaaccac agtctataca
     5821 agaaccagca tctataacat atgcgtcgtc tccagctgat atacagctaa cagggcattc
     5881 agcctcacaa gcaccacagc ttatacaagc gtcagtaatt ttatatgcca ttttgaaatc
     5941 ctccctttta tatgtattgt aattcaataa cattataaca aatattattg aatataacaa
     6001 ttaaattaat tctacaacaa aaactttatt atgatattct tgttttaaag ttttacaata
     6061 aagtttaatc tgcttatatt tttatttatt ttaaagaagt ttattttaaa aataaaatta
     6121 aatttaaaaa gttatgtatt gattatattt aaaaatgtta tactctaaaa aagtatattt
     6181 tatttaaaaa tttattaata caaaggagat ttactatgaa aaataaatta gtttacatat
     6241 gtattatgag tctattgcta tttgtagtag gatgttctaa taatccaaat aaggaaagta
     6301 ccattaaaaa agactctaag aataaccaaa gccaagttaa agaagaaaaa agagaacctg
     6361 tgaaaaaaga tataactata agtttcgctg gtgatgtaac tatggggaac tataaaggtt
     6421 catcatatta tggcagtttt gaccatgaat ttaaaagaca aggagaagat tacaattatt
     6481 ttcttaaaaa tgtaaaacct gtatttgaaa aagatgactt aacagtggtt aatttagaag
     6541 gtccacttac tacagcgtca aatgcaaaag aaaaaaaatt tgcatttaaa ggaaagcctg
     6601 gatatgtaaa tattttaaaa tctggaagtg tagaggcagt aagtattgca aataatcatt
     6661 cagaagatta ttttgaagaa ggaatgaaag atacacagtt tatattggac gagaataaaa
     6721 taaattattt tggattaggg aaagatgctg ttgtagatgt caaaggaatt aaagtgggtc
     6781 tattagggta taatggatta tcaactgaat ataatgaaaa aaatttaaaa caaatggaag
     6841 atgatattaa gtccttaaaa aagaaatcgg atgttgttgt agtgtacttt cattggggga
     6901 tagaattaga ttattatcca gataaaaaac aaaaagattt tgctcattat gctatagaca
     6961 agggagcaga tttagttatg gggtctcacc cgcatgtgat tcaaggaata gaaaagtata
     7021 aaaataagta tatagcttat agtctaggga atttttgctt tggaggaaat aagaatcctt
     7081 ctgatacaga tagttatata tatcaacaaa cctttacttt ttcagataat aaattaactt
     7141 caattaaaga gccaaatata ataccaactt caataacatc tagtaactcc agaaataatt
     7201 atcaaccaaa agtattggat ggaagtgaga aggatagagt tttaaataaa cttgaaaaaa
     7261 taagcaaaga tttaaataaa taaaatgtaa aaaaaaataa atatatatat ttattttaat
     7321 aatttatatt ttgtttaggg tatggtaaat cgtggtatta aataagtaga ttttaatagt
     7381 taaattatag tcaattataa taaagctatt taatctactt ttttaatata aaaatataag
     7441 ggggaatttg taatggcttg taacacttgt aaaatgtgcg aaagtgcaga caagaaatta
     7501 gaaagttttg tagcttcaaa ggatgttgaa acagcatttc atagaacaga agatcagaaa
     7561 gttaagtgtg gttttggact tcagggggtt tgttgtagac tctgttcaaa tggaccatgt
     7621 agagtaactc ctaaatcacc aagaggtata tgtggagcag atgcagacac tatagtagca
     7681 agaaactttt taagagcagt agcatcagga gcagcttgtt atttacatgt ggtagaaaat
     7741 acagctaaga atttaaaaaa tgttggaata actaaaggtg ttgttaaagg agaaaaaaca
     7801 ttaaatcaat tagcagagat gtttggaata gaaagtgatg aaaagtatga taaatgtata
     7861 aaggttgcag acaaagttat caacgatttg tataaatcaa gagatgataa aatggaatta
     7921 gtagaaaaaa tagcttatgc acctagagtg aaaaagtgga aagagttagg tataatgcct
     7981 ggtggtgcaa agtcagaagt ttttgatgca atagtaaaat catcaacaaa tttaaatagt
     8041 gacccagtag acatgctagt taattgtttg aacttaggta tatcaacagg attatatggt
     8101 cttacactaa ctaatctttt aaatgatgtt atgttgggag aaccagttat aagaatggct
     8161 cctgtaggat ttaatgtaat agacccagac tacataaata taatgataac aggccatcaa
     8221 cattcaacat ttgcaaattt ccaagataga ttaaaagatg aagatgttat aaaattagct
     8281 caatctgttg gtgcaaaagg ctttaagcta gtaggatgta catgtgttgg tcaagattta
     8341 caattaagag gagaacatta tcaagaagta tttgctggac atgcaggtaa caattttaca
     8401 agtgaggctg ttttatcaac tggagcaata gatatagttc tttcagaatt taactgtact
     8461 ataccagggc ttgaaccaat agcagataaa tataaagtaa aaatggtttg tttagatgat
     8521 gttgctaaaa aagcgaatgc agaatacata ggtttagaca gaagtaaatt agatgaactt
     8581 agtaacactt taatagaaaa agcattggag tcttataagg agagaagagg aagtattgaa
     8641 atagatatac caaaagacca tggatttgaa caatctttaa ctggtgtaag tgaaaagaac
     8701 ctgaaagcgt ttttaggaga ttcatggaaa cctcttatta atttaattgc agaaggaaaa
     8761 attaaaggtg ttgcagctgt tgtaggatgt tcaaatatga cagcaggagg acatgatgta
     8821 aatactgttg aacttactaa agaattgatt aaaaaagata taatagtact atctgcagga
     8881 tgttctactg gaggattaga aaatgttggt cttatgtcac ctggagcaga agaattagca
     8941 ggagaaaact taaaagaagt atgtaagact ttaggaatac caccagtatt aaactttgga
     9001 ccatgtctag ctataggaag actagaaata gtagcaacag aattagcagc tgaacttgga
     9061 attgacctac cacaattacc attagtgctt tcagctccac aatggttgga agaacaagca
     9121 ttagctgatg gagcgtttgg tcttgccctt ggcttacctc ttcatttagc tttacctcca
     9181 tttataacag gaggaaaatt ggtgacagaa gtattaacag aaaaattaaa agacttaaca
     9241 ggaggacatg ttatagtgaa tccagaccct aaatcttcag ctaatcaatt agaagaaata
     9301 ataatagata gaagaaaaaa tttagggtta aaggatgtgg aatgcaatgc atagaatttt
     9361 tataaataaa gatttatgta caggatgtaa gagctgtgta ctagcatgta tgctaaaaca
     9421 caataaagat tatgacatgt acactcttga tttagaaaat atagataatg atagtagagg
     9481 gcatatagaa ttagatagta aacataataa tccagttcca attctttgta gacactgtga
     9541 tgaacctgaa tgtgtattag catgtatgag tggtgcaatg cataaagata gtgaaagtgg
     9601 aatcgtttca tatgatgaag agaaatgtgg ttcatgttat atgtgtgtaa tgtcttgtcc
     9661 atatggatta ttaaagccag atgatagaag caagcagaac atacttaaat gtgatttatg
     9721 taaagatgag gagtatccta gatgtgtagc taattgccca agtggagcaa ttgaattaca
     9781 gaaggaggaa aatgatgaat tatgtagtgt taggagctag tgcagctggt ataaatgcag
     9841 taaaaacatt aagagaatta gataaagatt caaatatagt agtaatttct aaagatgaaa
     9901 acgtatactc aagatgtatg ttgcatcacg ttatatctga acacagaact ttaaaacaaa
     9961 taaattttgt tgatgaagat tttatggagc aaaataatgt aaaatggatt gcaggtaaga
    10021 ctgttaaagg tatagacata aataaaaagg tagttcagac agaagacatt actgtaaatt
    10081 atgataaact tttaatagct acaggtgcat cttctgctat tcctcctata aaaaatttaa
    10141 gagatggaaa ttttgtatat tctgtgagaa acatagatga tatttataaa ataaaagaaa
    10201 aagcacaaaa ttctaaaaat gtagttataa taggtgctgg tttagttggt atagatgcat
    10261 tagtgggatt atttaaatat gagcagttaa atatttctgt agcttttatg gaaaagtaca
    10321 ttttagatag acaattggat gaatatacag catcagtgta tgaaaataag ttcaaagaaa
    10381 aaggtgtaaa attttaccca agtgcatcaa tacaagaaat tgtattggat aacagtaaga
    10441 atgttacagg tgttgcattt tcaaatggtg aagttttaga tgcagacatg gtaattgtag
    10501 ctacaggggt taaaccaaat gctgactttt tagatggaac aggaatagaa tatgatagag
    10561 gtataataat agatgatatg tgccaaacaa cacaaaaaga catatatgca gcaggagatg
    10621 ttgtaggtaa aaatgctatt tggcctctgg cagtaaagca aggaattgta gctgcctata
    10681 atatggttgg aaaagataag aagatagaag atgaatttgc ttttaaaaat agtatgaatt
    10741 tcatggatat accaactatt tctataggaa tgaatacacc tgtagatgat agctataaag
    10801 tattaaccag acatggaatt aatgattata aaaagtttgt ttttaaagat aatgtaatct
    10861 atggagcagt tatacaagga gatatttcat atgtaggtgt attaacttat ttaataaaaa
    10921 ataaagttga aatatatgat ttagaaaata ggatatttga tatatgttat gcagactttt
    10981 ttaatataaa agaaaatgga gaattttgtt atagtgtata gtatatacgt ggagtaaggt
    11041 ttataaatac agtaaatcaa taaatagagt tattctaaaa taagaaaaat gacaaatctt
    11101 atttagaaat agctctattt tttgtaatta aatttaaatt atattcaatt tgtaaaatat
    11161 tattttgaac aattatacat atactctcta gttagaggta aatcattttt tataccttta
    11221 gtgaaaagta tttgatgtat atctataata ccagtgttga aagcagcagc acaagaacaa
    11281 aggtataaat cccacattct tataaactta tcatcaaaca tttctttaat ctttggaaga
    11341 ttctcagaga aatttttttg ccagtatagt aaggttttta cataatgtaa tcttagactt
    11401 tctacgtcta tagtatgata tttgtaatca ctacaaatat gtatcatttc tcttaggcta
    11461 ggaataactc ctcctgggaa aatatatttt tttatccaag ggtctccata agattcctgc
    11521 aatccactta tatagtgaag taaaaacaat ccaccatctt ttaaaacagc atttacgttt
    11581 ttaatgaaaa gttcatagtt ttctctacca acatgttcaa gcatcccaac gcttacaact
    11641 ctatcaaaca taaggtctga tttttttaat tctctatagt ccataagtct tacctcaaga
    11701 tagtcttgaa ggttctcttc tttaattctt gtgttaaatt ccttaaattg ctcttcgcta
    11761 agtgtaattc caacgccgtg aattttatat ttttttgcag cttctattag taaaaatccc
    11821 caaccacaac caatatcgag aaggctcata ccttcctcta aatttagttt agttaatatg
    11881 tggtgaactt tattgacttg agcatcataa agagtatcat tatcatttct aaagtatgca
    11941 caagaataac taagtgtctt atctaaccat aagctataaa aatcgttgcc aatgtcatag
    12001 tgggcatgta tttctttctt ttgatgtctc attgaatttg atgagtgcaa tagatgtttt
    12061 acagctgaat tatcaatatt gaatttgtct atttgtttta aaagtagatt taaagttaag
    12121 aataaatctc cctcaacttc aatgtctcta tttatgtagg cttctccaag tgctaaagat
    12181 gtacttttta atagttcaga cttgctaata tcattattta ctataacttt gaactgaggg
    12241 gagccctctc caattagaaa ttcttcctca tctttaagcc taactagaaa tggtgtttca
    12301 ccaaatctct ctaaatagtc ttttaaaaaa ttttttgtaa tattcatatt accagctcct
    12361 tctaatgtaa atccagtaag tttccctgct taaaatatat tatagtcctt tttttaaaaa
    12421 agtaaaattt tttcaataaa ttttttattt aaaggaaatt tattttttat aaagaaattt
    12481 catgttgtag tatatttttt gcttataaat tttggtactg gatataatat taaactgtta
    12541 atgatataat aaatttaatc tataaagtaa ataaaatttt gtaataaata gttaaagcta
    12601 ttttataagg agatatgtag gatggaagta tatgctttgg ttgggtcaag tggcacaggg
    12661 aagagcttta aggctttaga gttcgcatat gaaaatgata tagaatatat aatcgatgat
    12721 gggattttaa tatacaaaaa caaggtttta gctgggatat cggcaaaaca agcaaatact
    12781 actatagaag cagttaaaag ggctattttt tataatttgg agcatagaga agaagttaga
    12841 gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc aaaaaaaatg
    12901 gttaatcaaa ttgtagagag attgtcaatt ggaagggtct gtaagtttat aaatatacaa
    12961 gatataagta ctaaatatga gatagaaata gcaaaacaag ctagaaaaga aggaaatcat
    13021 gtaattcctg ttccagcagt tgagataaaa agtatggcaa gtggtcttag tataaattct
    13081 ttgaagagac tttttagaaa aggaaataat aggaatatgg tagttttaga aaaaacaatt
    13141 ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt aattaaacag
    13201 attattgaat atgaagtata caactttgga agcattgata gaataaataa aatcaatata
    13261 gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga tttagaaact
    13321 ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa aatgacttta
    13381 gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa cttcctccaa
    13441 tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg ctataattaa
    13501 actgtaaatt taggaaatgg ttttttctaa atggagtata cataattata acgtatttat
    13561 aagggggctt tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg caatctcaag
    13621 taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt ttatgaatta ttaaaagaac
    13681 ctatgagagt tatagaggtt tcaattccag taaaaatgga tgatggttct ataaaaactt
    13741 ttaaaggatt tagatcacaa cataatgatg cagtagggcc aacaaaaggt ggaataagat
    13801 tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc tatatggatg actttcaagt
    13861 gttctgtaac aggtatacca tatggtggag gtaaaggtgg aataatagta gatccatcaa
    13921 ctttatctca aggtgaatta gaaagattaa gtagaggata catagatgga atatataaat
    13981 taataggtga gaaagttgac gttcctgcac cagacgtaaa tactaatggt caaataatgt
    14041 cttggatggt tgatgagtac aataaattaa ctggacaaag ttctataggt gttataactg
    14101 gtaagccagt tgaatttggt ggttctttag gaagaacagc tgctactgga tttggtgttg
    14161 ctgttacagc aagagaagct gctgctaaat taggaataga tatgaaaaaa gctaaaatag
    14221 ctgttcaagg tataggaaac gttggttctt atacagttct taactgtgaa aaacttggtg
    14281 gtactgttgt agctatggct gaatggtgta aatcagaagg ttcttatgct atatacaatg
    14341 aaaatggttt agatggtcaa gctatgttag attatatgaa agaacatggt aacttattaa
    14401 acttcccagg agctaagaga atatctttag aagagttctg ggcttcagat gttgatatag
    14461 taataccagc tgcattagaa aactctataa ctaaagaagt tgctgaatct ataaaagcta
    14521 aattagtttg tgaggctgct aatggaccaa ctactccaga ggctgatgaa gtatttgctg
    14581 aaagaggaat agttcttact ccagatatat taactaatgc tggtggagtt acagtttctt
    14641 actttgagtg ggtacaaaac ttatatggat actactggtc agaagaagaa gtagagcaaa
    14701 aagaagaaat agctatggtt aaagcatttg aatctatttg gaaaatcaaa gaagagtaca
    14761 atgttacaat gagagaagct gcttacatgc attcaattaa gaaagttgct gaagctatga
    14821 aattaagagg atggtactaa gatttactct taataaataa taggatataa ttatttaaga
    14881 tataaagggg ctgtacatag tacagccctt aaattatgtg gcttacaatg aagtaagcta
    14941 tttttgtttt aacttataaa gaacttgata aattatgtaa tttagaatgt attttttacc
    15001 accacatgat tacaaattag aggagttaga ttgaattttt agacatgaac ttatgcattt
    15061 taaaagcaaa gactttcatt taaagtatct agtattattt ttaaaaatag tatattggtt
    15121 taatccattt atatataatg gaaaaatata tggatttgga ttgtgaattt tattgtgatg
    15181 agagagtttt aaaaaattgt actgtacaac aaaagcaaga atatgctcta actattgtaa
    15241 attcaatgaa aagaggtaca aatttttcaa ataaatttgt agctggatta aataaacaat
    15301 ctgatattag aaaaagagta ataaacatgt tcagtgaaaa atataaaaat ggtatcttaa
    15361 tggctttgat tataagcttg ttttcttcta ttacattttt taaattcgat tcaactagta
    15421 ttattgaaga gaatgatttc ttagagaggt caagatatac gtttgaagga aggcaagaag
    15481 acgtgtttgt aacaatagat tttatatatg cagatgcacc tataaaactt agaaaagagc
    15541 atgaagaaaa ctgtagagtc aatggaatag taccaaaaga ttcagattca atagaaatta
    15601 ttcctagatt ttataaagat tgtatggatt taaaaaaata agttagactt aatattgatg
    15661 ttgatattat gaatttttta tatttaagca attgaaaatt ttattataga ttcaatttta
    15721 tacgtgttta agtatgaaat ttatagatag ttgaaataat taaatatgat tgatgcatag
    15781 gaatcattac taagctataa ttaattatag gggagtttaa ataaattatg agttacacaa
    15841 ttataaaatg ataaaaatag ttatctgtta caaaaaatgt atctttttaa ttaataaaga
    15901 taatatgaaa acttaaaagg caaataaact taaacataaa atactcttaa ataagatagg
    15961 agaaaaatgt atgtctaact ttattctaaa tgggattttt agaacagtta tagtaagtag
    16021 tctaagcatt cttatgatat tgattttcaa gaaaaatgta tttaaaagat ttagcaaaaa
    16081 atttaattat tatatatgga tgatagttgt tattaaatta tttttaccat ttacatatta
    16141 tacttttaca ataaatatct tgagaagtaa aaaacacatt aatattaata acataaattt
    16201 agagggattt aataatgttt caactataag ta
//
LOCUS       DAENCV010000083        16176 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAENCV010000083 DAENCV010000000
VERSION     DAENCV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.084"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            127..627
                     /locus_tag="KQD82_002645"
     CDS             127..627
                     /locus_tag="KQD82_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG4381107.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            684..1670
                     /locus_tag="KQD82_002646"
     CDS             684..1670
                     /locus_tag="KQD82_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG4381108.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1720..2442
                     /locus_tag="KQD82_002647"
     CDS             1720..2442
                     /locus_tag="KQD82_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG4381109.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2471..4405
                     /locus_tag="KQD82_002648"
     CDS             2471..4405
                     /locus_tag="KQD82_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381110.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYISEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMALNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4398..5666
                     /locus_tag="KQD82_002649"
     CDS             4398..5666
                     /locus_tag="KQD82_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG4381111.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKHLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            5993..7033
                     /locus_tag="KQD82_002650"
     CDS             5993..7033
                     /locus_tag="KQD82_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4381112.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            7389..8711
                     /locus_tag="KQD82_002651"
     CDS             7389..8711
                     /locus_tag="KQD82_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG4381113.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            9008..10369
                     /locus_tag="KQD82_002652"
     CDS             9008..10369
                     /locus_tag="KQD82_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG4381114.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            10442..11836
                     /locus_tag="KQD82_002653"
     CDS             10442..11836
                     /locus_tag="KQD82_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381115.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            11898..12368
                     /locus_tag="KQD82_002654"
     CDS             11898..12368
                     /locus_tag="KQD82_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG4381116.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            12371..14587
                     /locus_tag="KQD82_002655"
     CDS             12371..14587
                     /locus_tag="KQD82_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4381117.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            14670..15341
                     /locus_tag="KQD82_002656"
     CDS             14670..15341
                     /locus_tag="KQD82_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4381118.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            15704..>16176
                     /locus_tag="KQD82_002657"
     CDS             15704..>16176
                     /locus_tag="KQD82_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HBG4381119.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNK"
BASE COUNT         6363 a         1626 c         3047 g         5140 t
ORIGIN      
        1 attaggaaaa tagttcgcta aaataatttg tttttataaa attactgttt aagtttatag
       61 attttgtata aaataaagtt atgttattaa tttttttaga aataaaactt ttactaggag
      121 acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat
      181 gcagttttaa cattatcatt gggatttata agttatggac caatacaatt tagggtagca
      241 gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt
      301 tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta
      361 gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa acagaataaa
      421 agtactttag ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta
      481 atgttaaatg tattctttgg gcttccatta attctatcta tgatacaagt aggttttgga
      541 gaatttgtag tagtaacaat tgtaggagta ccatttttct gctttttaat gaaaaaatat
      601 aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat
      661 tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat
      721 atattaatga taagcaaaag tttacaataa aagagttgtc agaagaattt aatctttcag
      781 ctaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag
      841 aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat
      901 ttacagaaaa agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt
      961 ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa
     1021 gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata
     1081 ctaatgatgg agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat
     1141 ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc
     1201 ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg
     1261 ataaaataat attagttcat atagataata taattgatat aaaaagaaca ggagaaagta
     1321 aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa
     1381 atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag
     1441 aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg
     1501 gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt
     1561 tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt
     1621 atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa
     1681 tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt taataagaat
     1741 gctaaaataa ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa
     1801 ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat
     1861 gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa
     1921 catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct
     1981 ataggaatgg attgttcaga gaaggaaaat aaagaagatt tagagcttgg gagagagttt
     2041 aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct
     2101 atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga
     2161 gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata
     2221 gctgacattt tatttgatag gatggaatat attggtgagg aatttataag taagaaagga
     2281 attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt
     2341 aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa
     2401 aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag
     2461 gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt
     2521 ttatcaggaa acagacacac cacagatttt tagagaagaa agcataattg cacttggatt
     2581 tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat
     2641 taatgagaat tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga
     2701 ttatttttta tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat
     2761 tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac
     2821 actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag tatatataag
     2881 tgaattttat aaattaaata ataatgatat tagctcatat ttagaaatta ttaagtcaca
     2941 agataagaaa ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa
     3001 aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc
     3061 cattaatcta gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt
     3121 gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt
     3181 atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt
     3241 ttttcctgtt agatggatag agtatgaaaa ggagaatata gacggaaatg catgggttat
     3301 atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatggcatt
     3361 aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt
     3421 ttacaatcta gtaagtgaaa ccagtctaag aaacagatac tatacaggtt acttagatta
     3481 taaaaacaat gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga
     3541 gggtcttaag tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat
     3601 atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga
     3661 aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa
     3721 tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga
     3781 tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga
     3841 tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa
     3901 attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat
     3961 agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta
     4021 tgatttattg tctattgtag atggtataga tattagaaaa atgctatata caatcaataa
     4081 aaggatagag tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt
     4141 agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga
     4201 gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga
     4261 aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa
     4321 taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga
     4381 ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa
     4441 gggtaggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaagcatc
     4501 tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta
     4561 gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa atacttccta
     4621 aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag atgctttcta
     4681 tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata
     4741 atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag
     4801 gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat
     4861 tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg
     4921 atgagtatag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt
     4981 tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc
     5041 aaaatgtaga tttaatttat atagatagaa aaaaactgtt ggattataaa ttttataaaa
     5101 acaatgatag atttaaagat tgttatttac tagctagact tctactttta aatttgtcta
     5161 tggataatag tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt
     5221 atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat
     5281 atatacaaga taagagtaaa tttcttttga aaaatgaaca aactggtaag aaaaacttta
     5341 atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca
     5401 ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc
     5461 agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta ttatatcctt
     5521 gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt aagggtaagt
     5581 ttatagaagc aaaaactgtg aggttagatg atataaaaaa cacaaaaaat gatttaaaaa
     5641 agataatttt taattataaa ttttaaaaat aagaatttta aaatttataa aagtaatttt
     5701 atataggtat aaaaattacg agtagtataa aaaatttaat aaggatttaa attaaaaatt
     5761 ttaaaaatta tttccatata aaatatggca ttgtaattgc ttatttaata gaggattttt
     5821 gtaaaatgaa gaaagtatat ctatcaaatt aaaactaagt agatatactt tttattaagt
     5881 tttgttttaa aataatatta ttaaattata actaagcttt tctgttatga tataattaaa
     5941 tagataatgt gaatgattat tatatagaat acttatttgg agttgatatt ttatggacaa
     6001 acacagtgat tcagtaatgt atggtggaat ggaagtatat aatatttggg gaacaacaac
     6061 agtagactcc ataattggag agtctgatgt aatgcgtgct cttaagaata gaatttgtaa
     6121 aattggaaat agtgattcta ctgtagctat aactggagaa agtggcactg gaaaagaatt
     6181 aatagcaaga gccattcatc tcgcaggaaa cagagggaat aaaacattcg ttgctataaa
     6241 ctgtgctgct ataccagaac ctttactaga aagtgaactt tttggatata caaaaggagc
     6301 ttttagtgga gcagattcaa aaggtaaaat tggtaagttt gaattggcca atggaggggt
     6361 tgtattttta gatgagatag gagatatgcc aattcaacta caatcaaagt tattgagggt
     6421 attacaggaa aagaagttta cgagaatagg ttcaaatgaa ctaattgata tagatgttag
     6481 aatcatatca gctactaata gagatttatt tgagttggtt aaggaaaata aatttagaga
     6541 agacttatac tatagattga atgtaatacc tttagaagtg cctccactta gagaaagagg
     6601 agacgatata caaatattaa taaacaattt tatagacaaa tattcgagaa agatgaagaa
     6661 aaacgtgtac catatagagc cagatgtgga acaaatgttg cttaagtatc cttggccagg
     6721 aaatattaga gagttggaaa atactataga actggcatta aaccttcttg aggaagatgg
     6781 aataatacat aaaggaataa taaatagaaa gatacttgaa tattttaagt taaataatat
     6841 aaatataaag ttacttgagg aaagtgacta tgagataagt atggaacaag acatacttac
     6901 acttgaagag tttgaaagag cctatataaa gaaagtgtta aagttttacg gaaatgatac
     6961 taagtctaaa aaactggctg ccaaaaaact tggaatatca ctagctacat tgtacagaaa
     7021 aatagatgta taataattag gctttttatt ctcaattcta aaatttgaaa ttataaaatt
     7081 gataaaaaac acttggaaaa ctattttcta agtgtttttt tgtgtaatag cattttatta
     7141 tccaaaaata aaaaaaattt aaaagaattt aaaaaaataa atgctttaat aaattatcaa
     7201 atactaacaa atataataaa aattatatat gtaaaacaat tatgttaaaa aattggcatt
     7261 ataattgctt catattaaat tgagatgaca aaataaaaaa agaaaggatg ttgtgtgaga
     7321 taataataac tgtcattaaa aaatagttgg gagtgataac cttgagtaaa tatcaaaaga
     7381 atgtttctat gtttgatatt gatggaaggc caccaatttt aaagtgtttg ccactatcaa
     7441 tgcaacatat actagcaatg atagttggaa ctgttacagt acctatagtc gtaggaggag
     7501 ctgtgggtgt tagccctaca caaataacac ttttagtgca atatgcatta ataatagctg
     7561 gtatatcaac acttatacaa ctatatccaa taggaaatat tggttcaaga ttgcctataa
     7621 tttttggagt tggttttact tatgtaccaa cacttgtagc agtcggagga aattatggat
     7681 tagcaagcat tttgggtgca caattaatag ggggagtggc tacagtatta atagggatat
     7741 tcataaaaaa gataagaaaa ttttttccga atatagttgc aggaacagtg gtttttacaa
     7801 ttggattatc tttgtatcct atagcaataa attatatggc aggtggtgta aatagtccta
     7861 cttatgcctc tatgcaaaac tgggcagtag cggctataac attagtggta gttataggtc
     7921 ttagtcaatt tgcaaaagga tatgcaaaat tggcagcaat atttttggga attatagttg
     7981 gatattgtgt atctttagtt ctgggtataa taaattttga ccctattaga gaggcaactt
     8041 ggtttgcgat acctaaaccg cttgaatttg ggatggaatt tgatatgcct gtaataatct
     8101 cagtagtaat tgtaagtata atcaatgctg ttcaagcaat tggagattta tcagctacta
     8161 caatgggagg catggatagg aatatcacag ataaggaatt atcaagtgga gtaataggaa
     8221 gtggaatttg tactatgata ggagctttgt ttggaggttt accaccatca tcatatagcc
     8281 aaaacgtggg gatggtagct atgaacaaag taataagtag atttgtgctt ggaatagctg
     8341 gtatatttat gttattgtcg gggtttgtgc ctaaatttgg tgcattaatg actactatac
     8401 catattctgt tttaggagga gctacaatat ctatatttag tatgataaca atgacaggta
     8461 taaagttaat tattacagaa gagttgtcat caagaaatat taccattgtt ggtttggcaa
     8521 ttgccttagg catgggaata acatcagtac cagcagccca agaggcattt ccttcatttg
     8581 taaagttagt atttggagaa tctccaatag taattgcaac attggttgca ttcgttttaa
     8641 atctaataat accaaataaa agtctagagg atgaggctaa agaaagagct gttatggaag
     8701 agactaatta agatataata atttaaaaga attgtttaaa ttatttagta tgagattgtt
     8761 agattatttt tgatagctat tcattttaat aaaaaattta acaaataaat atataccagg
     8821 caattaatgt tcatttaagt tgaaaatcgg ggagggaata tttagaaatt tgttaatttt
     8881 ttataaaaat agctcagttt agctgtgttt atcggttgag caataaattc atggtcataa
     8941 aaatatttat agaaaataat tataaaaata tttagtcaag atatttatgt atgggaggat
     9001 ttattttatg aaaacacgta atacttcaaa atatgatgtc gatgggatac caccactaaa
     9061 ggaatcaata cctttggcct tacaacatct gttagcaatg atagtaggaa atatggtacc
     9121 tgcgatatta atagcaaacg tggtaggact taaccagggg caagcaacaa tgttgattca
     9181 gggttctatg ttagcagcag gtttagcaac gtttttacag ttatatccaa tacctttatt
     9241 taaaggattt aagttaggtt caagattacc tgtaatgatg ggaatgagtt atgtattttt
     9301 aggagcttgt ctttcagtag cagctaaaga tggtttagca gctctatttg gagcacagat
     9361 tgctgctggg gtaatcgtat tctttgtagg atttggagtt aagaaaatta gacatatatt
     9421 cactccaata gtttctggaa ctataatagc atgtatggga cttggactat tcgcaacagc
     9481 tataaagaat ttagctggag gagaaggcac acaaacattt ggaagcccaa taaacttcac
     9541 agtaggagtt atagttgctt ttgtaataat aatgataaat aaatacggta aaggtttagt
     9601 aaaaaattct tcaatattaa taggaatatt agtaggttat gctatatctt tagttttagg
     9661 attggttgat ttctcagccg ttcaaggagc agctatagta tctttaccaa caccagcagc
     9721 ttttggatta gaatttagac cagagttaat agtaatgttt actattattt acataatagg
     9781 tatagctgat atgatgggtg cctgtacaat agctacagta ggagcaatgg atagagaggt
     9841 aactgacgaa gaattagcat cagttgtttt aggaaattct ataacttcta taatttcatc
     9901 actatttgca gctcttccaa ctggagtatt tagccaaaat acagtaattg tatctatgaa
     9961 caaagttaca agtagatttg taatagcact aggggcactt gtattattac tagcagggat
    10021 atctccagca ttgggtgcaa taatgacaac tataccatct tgtgttgttg gtggagcaac
    10081 tttagtagtt ttctcatcaa tagcaatgtc tggtttctct attatgagta tggatggatt
    10141 cactgaagaa aacaatctta tagcaggtgt atctatagca acaagtatgg gtcttacaac
    10201 tgctccacaa gttttagacc aattcccaga aacaattgga actgtccttg gagggtcaag
    10261 tatagtttct ggagcaataa tagctatatt acttcaaaca ttatttaaat taaagtcacg
    10321 taaaagtgct gaaaacgtta cttcaaatct tgaggaaaat attggataaa tgtattagat
    10381 ataatatgag aaaaccatat ggtaaaatgt tgtaatgaaa gtattcaaag ggggatttaa
    10441 gatgattgat atactaataa aaaatgcaat aattgttaca gttaataaag acagagatgt
    10501 tatatttgat ggagcaatag ctattcaagg tgacagaatc ttggacatag gaaatacagt
    10561 tgatattgag cctaagtatc ttgatgctaa aaaagttata gatgctaatg gaaaagtaat
    10621 attccctggc tttataaata ctcataatca cctgttccaa gtgttattaa aaggtttagg
    10681 tgatgatatg gcacttcatg agtggttaaa tacaatgatg tttccatcag caaaattctt
    10741 aacagaacaa gatacttatg atgctgcaat gcttggatgt atggaaggtt taaaaagtgg
    10801 tataactaca atggttgatt atatgcatac tcacaataga ccaggactta cagatggaat
    10861 agtaaaggca tataaagatt taggcataag aggggttgta ggtagaggtt gtatagattt
    10921 aggtattcac aaagagctta tagaagatgt ggaaacggtt gaaaaagatt tgagaagggt
    10981 attcgaaaaa tatcataatt cagaaaatgg aagaataaaa atctgtgtag ctccttcttc
    11041 aatgtgggct atatctgaag aaatgggtaa gatgctttgg aatatagtta aagaatatga
    11101 ttcatatttt acagttcata tatctgaaac agaatttgca agaacagcaa caaaggatat
    11161 acatggagaa atagatataa aattacttga aaaatggggt atagtaggtc cagaagttgt
    11221 agcagtacat tgtgtatgta taactgatga agacatggaa atgcttaaaa agtatgacat
    11281 aaaagtttct cataatgtag caagtaatat gtatcttgct tctggtgttg cacctgtgcc
    11341 agaaatgtta aaaaaaggta taaatgtgag tttaggtctt gacggtgcag caagtaataa
    11401 cgcacaagat atggtagagc ttatgaaact aacagcttta caacacaaag taaataattg
    11461 tgaccctctg gcaatttctg cagagaaagt tcttgaaatg gctacaattg agggagcaag
    11521 aactcttaga atggaagatg aaataggttc tttggagatt gggaaaaaag ctgacctagt
    11581 aatatttaac tcaatgcttt ctccaaaatc aatacctctt cacaatcctg tatcaacttt
    11641 ggtatactca gctagtatgc acaatgtaga gagtgtttta gttgatggaa atgtaatact
    11701 tgaaaatgga aaagtaacta caattgaaag tgaagaacaa gtgttgctta atgcacaaca
    11761 tgcagcagaa gaattatgta agagagctaa tgtaactaat agaatggaag gacataagtg
    11821 gaatagctta tactaatatt aaaataagat tgtaataata ttctattaca acattagttt
    11881 atctgtagga ggagaaaatg gaaaaaataa aattaaatat taatgattac atgtatgaaa
    11941 tagaagttga aaagaatgaa actcttttgc atgtacttcg tgaaaaatta aatctaactg
    12001 gagcgaaaca aggttgtaat agtggcagtt gtggagcatg taaagttttg atagatggag
    12061 ttgacatgaa atcttgtaaa cttcttgcaa aaaactgcga aggtaaagtt atatacacta
    12121 tagaaagttt tgctaatgga gaaaatcttc atccaataca agaagctttt atagaagttg
    12181 gagctgtaca atgtggatat tgtactcctg gtatgattat aacaacacaa gctcttttaa
    12241 atagaaatgc atctcctaat gaagatgaaa ttagagaagc ttttaaagaa aatctctgta
    12301 gatgcacagg atatgtaaag attatagaag ctgtaaaatt atctgcagct agattagggg
    12361 gtagataatt atggaaaagg taataggaaa atcataccct ctaaaagatg cagctttaaa
    12421 agttactggt caaatgaaat acgtggctga tatgaaacca caaaatgtac tttacgctaa
    12481 gatgctttta agccctgtag cacatgcaaa aataaaaagt atagatacaa gtgaagctga
    12541 gaaagtagta ggtgttagag ctatagctac ttatctcaat acatcacaaa gaaaatacaa
    12601 tggttcatta agattttatg agcacaaaat acctgaaaat gaagttatat tttcagatac
    12661 agttagattt gttggtgacc gtgttgcagc agtagctgca gagactccag aaatagcaga
    12721 gaaagctgta aaacttataa aagttgaata tgaagaattg ccagctgtat ttactataca
    12781 agatgcaata aaagaagatg ctatctgcat acatggagat tcaaatatag tttctcaaaa
    12841 caacataaat gggggagatg tagaaaaagg cttcaaggaa tgtgactata tatttgaaga
    12901 tgaatactct actccagcaa tacatcatag tgcaatagag actcatagtg taattgcaca
    12961 ctatgattat aatgataaat taactgtaca tacaccttgc caaaatacat ttggatttag
    13021 aataatacta tctcaaattt ttgggatgtc attaaataaa gtaagagtta taagacctgc
    13081 tattggaggc tcatttggtg gaaaattaga acttacaatt gaaccagtag cagccgcatt
    13141 atcaaaaatg acaagaagac cagttaaaat ggttcttaca agaaaggaat ctatgatatc
    13201 aacaagaact cgtcatgggt cttttgtaaa attaaaaact ggtgtaaata aagatggtga
    13261 agtaatagca caagacataa aaatatatac aaatacagga gcatatgcat caagtgcttt
    13321 aaatgttata ggagcactta gtcataaagt atttaaagtt tataaaatac caaatataaa
    13381 gtttactggg atgcctgtat atacaaatac accaatagca ggagctatga gaggctatgg
    13441 gtcacctcaa atatttatgg ctcaacaagc tcaatttgcg aaaatagcaa aggaaatagg
    13501 tatggattta gtcgattttc aaaataaaaa tgcagtagag ccagatgatg tcgatataat
    13561 cttccacggt tctcttggaa atcctagagt attggattgt atagagcaag gtaagaagat
    13621 gtttaagtgg gatgaaaaga aaaaacttcc aaaagaagaa ggaaaatatt taagaggtat
    13681 aggtatggca ataggtgcac atggtaatgg agtttttggt gcacatagag atgttgttac
    13741 attgactctt aaattaaatg aagatggtac gttgacacta ctaacaggag ctcatgatat
    13801 gggtaatggt gttgtaacta tgcaaacaat gatggtagct gaagttttag gaataacacc
    13861 tgataaagtt gatacatttg aaactgatac ggatgcatgt acattcaacc ttggtgacta
    13921 tgcaagtaga ggggtatttg tagaaggtgg aggagctaaa aagacagcag agaaattgaa
    13981 aaatcttata ttagaagaag cagaaaaatt acttgaaaca tcaaaagaag atttatatct
    14041 agacaatgga tgtgtaatta gtaaacttga tgaaaatgta aaagcatcac tttctgatgt
    14101 cgcagtatat agtcaaagta aatcattaag agagttaact gttgttgaag attattcttc
    14161 accagctgga cgtacttctt atggtgtaca ttttgctgaa gttttagtag ataaagaaac
    14221 taaggatata aaagtagttg attttgtagc tgtacatgat gttggtagag ttataaatcc
    14281 tctaaattta gaaggacaat tagaaggtgg gattcatatg ggtctaggat acgcattgag
    14341 tgaagaattg gcatttgatg aacaaggtag acctaaagct actgatttta aaacatataa
    14401 attatttaga actgtagata tgccaaaatg tcaagttgca tttgtagatg gttatgaaaa
    14461 agctgggcct tttggtggta agagtattgg agaatgtgcc gttgtaccag ttgcgccagc
    14521 tgttgcaaat gcagtatata atgcaactga tgtagagata cattcattac caataaaatt
    14581 aaaatagtag aaattaaagc agaaataaca atttattgta tttgtaaata gataataaaa
    14641 atagatttat ttggtgagga gatattatta tggcaaagat attaggtatt tgtggaagtc
    14701 cacgtaaagg agcaacagaa tatgcagtgt tagaagcttt gaaagaagca gaaactatac
    14761 ctggtataga aactgagtat tggtcagtta gagggaaaaa aataagtcct tgtgtacatt
    14821 gtgatgcatg tataagaaaa gaaactatgt gtattataaa agatgatatc caagaattag
    14881 agcaaaaaat attggaggca gatggaatta tagtaggttc tccagtttat gatatgaata
    14941 taactgctca acttacagca gtatttaata gacttcgtcc aatgtattta gttcatccag
    15001 gaaaacttca aaataaggtt ggtgcagcta taactactgg tggaactaga tatggtggcc
    15061 aagaattgac aaaacttcct ataataaatt tcttcctaat gcatgaaatg ttagtgtctg
    15121 gaggtttagg tggatgctat ataggtggta caatatggag taaagatgga aaagcaaaag
    15181 gtgcccaaga ggatgagaca ggtatggaca cagtaaaaag acttggaaaa ggtgcagcag
    15241 aggcagttat ggtttctaag tttggtcttg aaaaatggaa tgtcgtaaaa gaagaattag
    15301 atgttaaaaa agatgaaaaa tcacctctta gagaccacta aagatttatt atattattga
    15361 atatatacaa atagtgatgt aatagattat tgcatcacta ttttatgttt taatttagat
    15421 tttataaatt ttatattaaa aaatatagaa acaaagtgtt ttgagattta tttttaaatt
    15481 ttaaaaatga gaattaaaaa gtgagaagat agaaaaggtt ttttagttat taaattctac
    15541 atacattgta tgaactaaag atagaaaaga aaaaataata aaagcattgc aatttctata
    15601 ctaaagagtc atggaaaaat atctagtata tatttagtat tggcatgtta attgcttata
    15661 tattaaagta taaaaattta acaataattt ggaggtattt tagatgttaa aagaaattaa
    15721 atggaaagta aataatttgc caaagggcga taaggaaaat tgtatcaaat ttttaaatga
    15781 agaagaaata actaaggtta gaaattttca caaaagcttt cctcaatata aagaaactcc
    15841 attagcaaac ctagaaggtt tagcaaaaaa attaggtgtt gcaggagtat atgtaaaaga
    15901 tgaatcttat agatttggat taaatgcatt taaagtatta ggtggttctt attctatggg
    15961 tagatactta gctcaaagat tagatacaga tatatctgag ttagggtatg acaaattaac
    16021 ttcaaaagaa ataaaagaaa aattaggaga aataactttc tttacagcta cagatggaaa
    16081 tcacggtaga ggagttgctt ggacagctaa taaattaggt caaaaatctg ttgtattaat
    16141 gccaaaaggt tcttctgaat ttagattaaa taaaat
//
LOCUS       DAENCV010000084        15933 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAENCV010000084 DAENCV010000000
VERSION     DAENCV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.085"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(98..325)
                     /gene="acpP"
                     /locus_tag="KQD82_002658"
     CDS             complement(98..325)
                     /gene="acpP"
                     /locus_tag="KQD82_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG4381120.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            497..922
                     /locus_tag="KQD82_002659"
     CDS             497..922
                     /locus_tag="KQD82_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381121.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            970..1176
                     /locus_tag="KQD82_002660"
     CDS             970..1176
                     /locus_tag="KQD82_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381122.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            1358..1537
                     /locus_tag="KQD82_002661"
     CDS             1358..1537
                     /locus_tag="KQD82_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381123.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            1571..1771
                     /locus_tag="KQD82_002662"
     CDS             1571..1771
                     /locus_tag="KQD82_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381124.1"
                     /translation="MLDRFFGEEGGFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            2024..3040
                     /gene="ccpA"
                     /locus_tag="KQD82_002663"
     CDS             2024..3040
                     /gene="ccpA"
                     /locus_tag="KQD82_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG4381125.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(3163..3561)
                     /locus_tag="KQD82_002664"
     CDS             complement(3163..3561)
                     /locus_tag="KQD82_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381126.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            3773..4678
                     /locus_tag="KQD82_002665"
     CDS             3773..4678
                     /locus_tag="KQD82_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381127.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            4793..4973
                     /gene="ssrS"
                     /locus_tag="KQD82_002666"
     ncRNA           4793..4973
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KQD82_002666"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5156..6373
                     /gene="cdeC"
                     /locus_tag="KQD82_002667"
     CDS             5156..6373
                     /gene="cdeC"
                     /locus_tag="KQD82_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG4381128.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(6473..7795)
                     /locus_tag="KQD82_002668"
     CDS             complement(6473..7795)
                     /locus_tag="KQD82_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG4381129.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(7846..8640)
                     /locus_tag="KQD82_002669"
     CDS             complement(7846..8640)
                     /locus_tag="KQD82_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG4381130.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            8750..10195
                     /locus_tag="KQD82_002670"
     CDS             8750..10195
                     /locus_tag="KQD82_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4381131.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            10224..10433
                     /locus_tag="KQD82_002671"
     CDS             10224..10433
                     /locus_tag="KQD82_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG4381132.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            10453..11847
                     /locus_tag="KQD82_002672"
     CDS             10453..11847
                     /locus_tag="KQD82_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG4381133.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKGI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            12087..12471
                     /locus_tag="KQD82_002673"
                     /pseudo
     CDS             12087..12471
                     /locus_tag="KQD82_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(12381..13547)
                     /locus_tag="KQD82_002674"
     CDS             complement(12381..13547)
                     /locus_tag="KQD82_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4381134.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETVDILEGILQ"
     gene            complement(13949..14170)
                     /locus_tag="KQD82_002675"
     CDS             complement(13949..14170)
                     /locus_tag="KQD82_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381135.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTISILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            14454..14591
                     /locus_tag="KQD82_002676"
     CDS             14454..14591
                     /locus_tag="KQD82_002676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381136.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(14628..15023)
                     /locus_tag="KQD82_002677"
     CDS             complement(14628..15023)
                     /locus_tag="KQD82_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381137.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            15202..15465
                     /locus_tag="KQD82_002678"
     CDS             15202..15465
                     /locus_tag="KQD82_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4381138.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            15434..>15933
                     /locus_tag="KQD82_002679"
     CDS             15434..>15933
                     /locus_tag="KQD82_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBG4381139.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSIL"
BASE COUNT         6123 a         1879 c         2540 g         5391 t
ORIGIN      
        1 atattgtaaa taaatgtaat ctagtctgtg cgaactctaa tataatttat tatgtagaag
       61 tttacacaga ctatttatat ttgtagacaa aacgatacta tttgttttct tcgatatatt
      121 tacatatatc accaatacat ttaaagttct cagcttcctc gtctggtatt tctattccaa
      181 attcatcttc aagggccatt ataatttcaa cagcatctaa tgaatcagct tctaagtcgt
      241 ccattaaaga tgcttccata gttatctcat ctaaattgtc tacccctaat tgttctgcta
      301 ttatttcttt tattttctca aacattttaa gcctcctaaa gcagttttaa ttaattttaa
      361 tgttagttta atataatatc aagaattttt caatatggaa tctataaaaa ataataaaat
      421 tttataaatg tagtaataaa gtattatttg atgaataaac tttattgata acaaatataa
      481 cttaggagga aatgctatga aatatgtagg actattacta acttcagtag gaatgttttt
      541 acttatagca gtaaattttt attataattc aataacttta gatatgcaac gtatagagga
      601 ttatgtaatg gagaccaatt taatccttga agatgtagct gaaaaggaga gttatgtatc
      661 taatgaaaaa gaagactaca tatcaaggtt aatgcatgta aagaaaggaa tagaaaattc
      721 aaaaacatcc ttcttgatag aaagatataa ggagtacaaa ataaaatcaa tagaaagttt
      781 aatctatact atatcagagg agaaaaaaga ttatctggat gaagttgata gatacaataa
      841 acttggtgaa aaagaaataa ataaactaat caataaaaac tttttagaag taacttatct
      901 gtctattact acatatattt aattaaagag acaaaactta gtctcttaat gattcaagga
      961 ggattagtta tgacaaaatt agaagacaat atggaacgtt tctttggagg atgcgaagga
     1021 ttttttggga ataaaatgtt gataataata gttatagtat tcttactatt atgcacagac
     1081 attttagaag acttattatg cgatgacaat atatggatat gggttatatt aatagtattg
     1141 ttattattta acttcgatga tggttgttgt tgctaaaaaa tattgattat taaataggta
     1201 aaaccagtaa cttatacaag ttatggtttt acctttttta gttcaaatta aaaaatttaa
     1261 aaaaatagta aaaatacatt agcaaaatgg aaaccaaatg catagtatat gttaggagcg
     1321 aaaagctcaa caaaatatca acaggaggta acgtataatg ttagatagat tctttggaga
     1381 agaaggatgt tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc
     1441 ttttggagat agctgggcag aatttgacat aatggcatgg ataccattcc taatagtttt
     1501 attaatactt tgctgctgtg gtggatgctt agaataagaa cacagttatt aaaaaaagga
     1561 gggctttgtt atgttagata gattttttgg agaagaagga ggctttggtg gaggaggatg
     1621 gtggataata gtactattct tcctattctt agcttttgaa gattgctgga gagacattga
     1681 cataatgtca tggatacctt tcttaatatt attgctaata acttgttcat gtggtggatt
     1741 ctttgatggt gaagtaggtt gcggctgcta atacttaaat cattagaata aaaaaatgct
     1801 ggtattttat accagcattt ttttatttta ttttttaata tgatttaaat taagaatgaa
     1861 tattattaaa tttgttgaat atagggtata atataaaata taatgtattt aaatgtactt
     1921 gcttttatgg taaaataaaa acttatataa atgtagttaa aataggaacc ttcaaaaatt
     1981 tggttcagtt ttagataaat ttagtaaaat aaggaggatg aagatgaaag gcaatataac
     2041 gataaaagat gttgctaaac aagcaggagt gtcaatatct actgtatcta gagttataaa
     2101 tgattcaaaa cctgtaactg atgaagtcaa acaaaaagtt ttagaggtta taaaagagac
     2161 tggatatata ccaaatccac ttgctagaag cttagtaaca aagaagagtc aattaatagg
     2221 ggtaatagtt ccagaagttt cagattcttt tgttaatgag gtgttaaatg ggatagaaga
     2281 ggttgctaaa atgtatgact atgatattct tttagcgaat acatactctg ataaggaaca
     2341 agaacttaag agtataaatc tattgagagc aaaacaagtg gaaggtatag ttatgatttc
     2401 atggatagtt gaacaagaac atatcaacta tatacaaaat tgtggaatac cagcgacata
     2461 tataagtaaa actgctagaa attatgatat atatacagta agtactagca acgaagaagc
     2521 tacttttgat atgacagagc atcttataaa gaaaggtcat gaaaagatag cttttataat
     2581 gacgagtaaa gatgatactg ttttagaaat ggaaagactt gctggttatg agaaagcact
     2641 ttcaaataac aatatagaat tagacaagag tttgattaag tatggtggaa ctgattatga
     2701 gagtggatac aatagtatga aagaactatt agatgatgga ataatacctc atgcggcttt
     2761 tgtaacaggt gatgaggctg ccataggtgc tataaatgct atatgtgatg ctggatataa
     2821 ggttccagaa gacatatctg ttgcaggatt taatgatgtt aagatagcta gaatgtatag
     2881 acctaaactt actacagtat atcaacctct atacgatatg ggagcagtag caataagaat
     2941 ggttataaaa ttaataaata aggaattaat tgaaaataag aaaatagaat taccttatag
     3001 aattgttgat agagaaagtg tcacagaaag aaaaaaataa aactattaaa gtcaatcagg
     3061 tagtgcctaa ggcactacct gattttttat gtcaataata tcaaatttaa taatagatta
     3121 actaaaaata attatctaat tacttaaata gtttattttt atctattcat gagggttaga
     3181 ctttttagtt ttcttaaata aacttttaga ttttttttgt tgactacctg gtgtattttt
     3241 tggcgcaggt gaatttgact tacctttagt aggtttagcc ttcttagttt tagctggttt
     3301 agtatctctt tggttttctg cgtctgagat tttttccatt atattatcca gttttttgcg
     3361 ttgattttca tctaaaaatg ctttgaattt tgatactact tcatctctgc catctaggtt
     3421 cttacctatt tttataagtt cctccataag ttcattttct gattttcctt tgtaactatc
     3481 agccatcttt tctattttat ccttgtcaat atttttttgt ttagccattt tttctaatga
     3541 ctctgtatct aattttttca taaaaaaacc cctttctata aatatatatt gaattatatt
     3601 catactattt atatatagaa ataatattga agtggtgaat tgtgagtgat atataatata
     3661 aataaaaatt cttatagata atatatatgc actgagcttt aaaaatggaa cattaaaata
     3721 tgaaaaacat actttaatat agggcaatta tataatataa gggggaattg tcttgaataa
     3781 cttactattt ttgattggag tgattgcatt gagtaatggt aatatctcta tatttgatga
     3841 taaaaataaa tcaaaaggaa ataatagagt tagaaaaaac aaaaaaaatg caaatgataa
     3901 actttcacag tctacacatg gcaatttaag gtttgatgca aatgatataa aaagaggttt
     3961 aaggctcatg gatttaagtg aagaagattt agaaatggga atggaaataa taacaagaac
     4021 taaaaaatac atgtcaagag atgaaagaaa aatactaata aaaatagaaa gtgttttaga
     4081 tttagttaga ggaataaaaa aattaaataa tatagatatg gtagatacag aagatgaaac
     4141 ggattttttc agaaaaatgg atagtgatga taaaaagaat atgatgataa aggaaattat
     4201 agatgtattc ccagaaaaaa gaaaaaattc agttgaaaaa gctataggaa tgaaaaagaa
     4261 gatagattta tttgcagaat tatttttacc agatgacttt ggtgaaggtg gatttagttt
     4321 gagctctttg gcaaatataa acaatcttgg tagtatgaat aatctcaaat tgttaggaag
     4381 tcttttaaga ggtgatgata gtaataatga tgatgaagac aataatgatg agttaaatta
     4441 tgaagacgaa gaattagatt gtgaagacaa agaattggat gatgatgagt taacagatga
     4501 aggtgaaatg gaggataatg aagatgaatt agtagaagat aagaacacag gctatgaaaa
     4561 tatagaaaat gatgaagata tagtatacga tgaggaacta tacaacatta atgagcaaga
     4621 atccgaatac aattataatg aaaaaaatac taaaactaga gctaagaaaa aacgataatt
     4681 aactaaatac ataaagaaaa aaggaagaaa aagtaatgat agaataagat tatctcaatt
     4741 actatataaa accactttaa aaaattcaaa atttattata taataataat atgaaatcct
     4801 gaaacatacg ataaggcttt tttaaattca acctacaaat gaaatacggg agaccgtgtc
     4861 tggtaaatta taacatgcct agttagtttg gacgttaaag gttactggca agtcacccac
     4921 ctgggagtgc ctagggtgtg aatttactta gccaccggtg tttcgggaca aaaaaagtgc
     4981 tactatttca aatagtagta ctttttttat taatatgcat ttttctacta aatacgccta
     5041 attattgata aattcaaaaa gcaagtaaca attaagatta taaaagcata gtattaatta
     5101 ggcttcaaaa agccaaagac ttaataagaa tcgcttacag ggagggcaat gaaaaatgca
     5161 agattataaa aaaaataaaa gaagaatgat gaatcagcca atgtctacaa tgaatgaaga
     5221 agaagtgtat acagatgaaa taaattcaga agacatgaga ggttttaaaa aatcacacca
     5281 tcataatgga tgtaatactg ataataagtg tgagtgccat gatgattgca atccatgcaa
     5341 cccatgtaat ccatgtaaac ctaacccatg caatccatgc aaacctaatc catgtgatga
     5401 caattgtgga tgccatgaca attgtaaatg tgattgtgaa ccatgtgaaa tggattcaga
     5461 tgaatgtttt gaaaacaaat gtggaccaga atgctgtaat cctatatctc caagaaactt
     5521 ctctgtatca aatgcagtgc catttgcaat agaggctaat agaatatttg atactatgca
     5581 attccaaaca tttacagatg caacaggacc aaatggagag ccattaactt ttgaaacaga
     5641 agtagtagaa gtatttggtt cagttccaag tgcaggtcaa gcaagtgtaa ctatagaaaa
     5701 aatatgctta agtaatgatg gaatcgttat agacacagga atgacaactt tagaagattt
     5761 cgatttagac ccattaggag atatagtagg aagaaactgt gaaacaactt ttgaatttgc
     5821 agtttgtgga gaaagaaact ctgagtgctg tagacaagga aaaggcaaat cagtagctta
     5881 taaacaaaga ggattaactg tagcagttcg taatttagta ctagagctaa gaggtagatg
     5941 tggatgtaca gagttcgttg cattagcttt cccagcagtt agagcaggag gtggatgtaa
     6001 gagaagagtt gattatgtag aatttacttt taacacactt tcagcaccaa tatgcttgcc
     6061 agctgacgga agagctgtta ctttaagaca agaatatcaa actaacttaa ctgtagattg
     6121 tataggaaaa tctatattaa aattagaatg caacgaatgt tgtgaacctt tctatgaatt
     6181 aattatacca aatgatatag atttagtact ttgcttacaa gaaacagtta gcacattaat
     6241 aagtgaacaa atagtagttt tagcatcacc aaatccaatc caaccaagac ttgttgatac
     6301 tttctctaaa gtatgtgatt tttcgcaatg tggacctaat catggaagtg gaaagccaag
     6361 ttgccacaga tagataggta gatatataga cagtttaaat tataaataaa aatcatctaa
     6421 ggaaggctac caaagtgtat aatttacatt ggtagccttc acgtttttat aactagcgta
     6481 tagattttag tgatttaaaa agcactatag tatgtaatag acaagtaaag attgatacta
     6541 tcgtcattgt gtatatgtat gcataaatat taagtcctgg aattggaaga aatacatata
     6601 aagctattag ttgaattaac atgccagtaa tagtatttat acttgaagct aattcttttc
     6661 ttattgaatg taaaatgcca gatagagttt gatttaggga cataaatagt ggaactaaaa
     6721 acatagcttt tagatattct cctgcaagtg tatttttaaa tactaatata cataaatctt
     6781 ttccaaagaa ataaaaaaat attgatgata gtatgcctac aaataaagta agaagtatgg
     6841 agtatttgat ttttttaatt acactcttac gctttcttag agccatttcc tgtgaaatag
     6901 ttggtattaa atttacaata agcgctgaac caacagtaaa aggtaaaaat ataaaaggca
     6961 ttaccatccc actgacaata ccatacatac ttaaagattg ttgataagtc attcctgata
     7021 atgctaatct tgatggtatc atcatagaac ttatagattg caaaagtatg tttgacattc
     7081 tattacaagt tatgggaata gacattttta atgtttccat agaagaatta taaaaatctt
     7141 ttatgcttat aatataccta ttgtcaaatg atgaatctcg ccataagcaa ataagcataa
     7201 atataatatt actcaactca ccaattgata tacctaatag agctatataa caattcatag
     7261 atttattatt tatgtacata acaagtaaaa aaacaaataa tattttacca atttgttcaa
     7321 tgatttgtcc aatagcaggg atttttacat tctttatgcc atagtaatat cctcttagta
     7381 cgtttgaaag agttattata actatagcag gacaaacagc tagtataaat agatttaatt
     7441 tagcatcttt taaaaattta agtgataaaa aagaactatt gaaagatgca actatagata
     7501 taattagtgc tacgaaaaat gatatatata aagttgatat aaataaagca ttagtattgt
     7561 gtctatcgtt taaagctttt ctctttgcaa ctagacaact aagtgcagta ggtatacctg
     7621 ttgttgtaac agctaagcaa atcatcaaaa agttaaaaat catatgatag atacctaaac
     7681 ctgtttcacc tagtgctctt gataagaata ttttatataa aaaaccaaat attcttacaa
     7741 taaaattgga tataaataaa ataactgttg atagtactaa ataaggtact ttcaaaaaaa
     7801 tcacctcctt atcttttaaa tatattcaca aggaggtgta aaaatttata attttataaa
     7861 ttctggtttc aatttattaa atttagatac atatttaaat cccatatttt ttagtaatga
     7921 atatatgtag tcaaactcac atgcaacttg acttatatga tgtgaatctg aacctgtggt
     7981 tatgatttcc cctcccaagt ctttatacat ttgaagtata tacttagatg gggttaggtc
     8041 tggaagatta tatctataac aagaggtatt tatctcgata cctttgccat cataaatagc
     8101 ttgtttaaga gtttcttcaa ttatatcaga aaatagtcta tcatctaaaa tagtgtcata
     8161 aggtgcatac cttttaatca aatctaaatg acctaagaca gagtaatctt tataattctt
     8221 aacaatatta tataaataaa gatagtaatt ttcataaact tcatgttgag ttttatcttt
     8281 aaaataatta ccaaggtata agtccattgt atctatagca tggatagaac atattataaa
     8341 gtccaatgga tattgatgtg cttccttttt gtatgtatcc attaattgag tttgaactcc
     8401 aaattcaatt ccttttttta ttgatatttt atctttatat ttgctttgaa gagtctctaa
     8461 actcttaaaa taatcttcat agacaattga aaaagaatca tcagcatata catcatagtc
     8521 aacatggtct gtgaaacaaa tttcctccaa accgagttca atagattttt ttaccatttc
     8581 ttccattgta gattttccgt ctgtagagaa acttgagtgc atgtgatagt cgtaaaacat
     8641 aaatatttcc cctttttata aaaattttgg tattatatat acatactaaa taatatcaaa
     8701 tatacataaa agcatcaata aaatataaat acaatgagcg gtgataaatt tggaaaaata
     8761 cagaatagat ttaaatgaga aaaaatcaac taaatatata cagatattta atcatataaa
     8821 agaattgata ataaataaag agttagtaga acatgaaaaa ttgcctccaa taagaaaact
     8881 atcaaatttt ttacaagtaa ataacactac tatagttaag gtatacgagc ttttagaaaa
     8941 tgaaggatat gtatataaaa ttatagggag tggtactttt gtatcaagtt taaattctta
     9001 taaaagcggt atagataaca ggaaaaataa gattattgtg caaagagaag atttaattca
     9061 ttttgacaat gggaatccat ctaatgatat gtttcctatc gattcattta aaaatgctgt
     9121 aaatatggca ttatctaaag atggtagttc aatatttgaa tatgatgaag gtcttggctc
     9181 tgaagaatta agagaaaaaa tggtagagta tctaagtgat ggaaatataa aaactactaa
     9241 ggaaaatatt caaataatat caggtgctca acaaggtata gatatagttt gtaaaggact
     9301 tataagttat tcagatgttg tttttatgga agaaccttct tacaatggag caattgaagt
     9361 gtttaaaagt agaggtgcaa aaataatatc tatacctatg ttagatgatg gaattgatat
     9421 aggtatttta aaattaaagt tggaaaaaat aaaaccaaga cttatataca ttatgcctaa
     9481 ttttcaaaat ccaactggaa tatcttactc aacatataaa aaaaagaaat tgatagaatt
     9541 ggcagaagaa catgattttt atataataga agatgatttt ataagtgatt ttgtttttga
     9601 atcaaaggac aatagaacaa ttagaagtta tgatgataaa aatagagtag tatatataaa
     9661 aagcttttca aaaatactta tgccaggtct taggatagga attgtagaaa tgccaaatga
     9721 gttgttaaaa aaagttttat gggctaaata ttcatcagat atatcaacac caggtttaat
     9781 tcaaaaatca atgtattact atatgaataa ttttgattgg aagaattatt tgagtcatat
     9841 agagaagata tatactgata agtataaatt ggcaaaaaac ttgattaatg acaaattgag
     9901 tggtaaatta aaagttagaa aatcttgtgg aggaataaac ttttttttag aattaccaag
     9961 aggatataca tctcaagcat ttacaaattt tatgttaaac aaaggagtgt caatgcttcc
    10021 agggacttat ttttttgaca atctagtgga tgatagattc tttaggataa atatagctcg
    10081 tccaagcatg gaagaactag aaaagggaat atctataata agtgataata tagatgagtt
    10141 tttttgtgaa tataaaaaca agttagatat aaaaagcaat aaattatttt attaaataaa
    10201 tagaggtttt ttaggaggaa aaaatgtttg acaagaagaa attagatagg attaatgaat
    10261 tagcaaagaa aaataaagag ggaatccttt ctgctgatga aatcaaagaa agagaaattt
    10321 taaggaaaga atatttagaa aactttagag cccactttag atcaagacta gatagtgtca
    10381 aagtagtttc accagaagaa tatgagcaat acatgaaaaa caataaaaat tagaacttag
    10441 taggaggaaa atatgaactt aaagtacgaa tttaagaatg cttgggattt tgaaagaaaa
    10501 gaaaatacta ttgaacaaat tatgcaatat tctcgcaatt atatggagtt tttaagtaag
    10561 tcaaagactg aaagactttc agtaaaagaa attataaaat tggctaaaga aaataactat
    10621 atttcaatag atgaagctat ggaaaaagga agcataagtt gtggtgataa gatttatgtc
    10681 ataaataaag agaaggcagt agcacttttt gtaataggta aaaattatat agaaaaaggt
    10741 atgaagatta taggaagcca tatagattca ccaagattag atttaaaacc aaatccactg
    10801 tatcaagaga gtaatctagg tttttttaag actcattatt atggtggaat aaaaaaatac
    10861 caatggacgg ctattccact tgctttacat ggtatagtta tattaaatga tggaacaaaa
    10921 gtggacatat ctattgggga agaagatagt gacccagtat tttgtgtaac tgatttattg
    10981 attcatttgg ctggagacca aatgcaaaag aaactatcag aaggaattag tggagaagct
    11041 ttaaatatat taattgggaa tattccttta gaagatgaag aaaaagaacc tataacagca
    11101 aatatattaa agattttaaa tgagaaatac aatatagttg aagaagattt attaagtgct
    11161 gaaattgaag tagttccagc tggtaaggct agagatttag ggcttgatag gtctatggtg
    11221 ttgggatatg ggcatgatga tagggtatgt tcatatgctg ctgtaaaagc tatactagaa
    11281 actgaacaac cagaatttac ttcagttgca ttatgtgtag ataaagaaga aattggttct
    11341 aaagggaata caggtatgca ctcaaagttt tttgaaaata ctgtggctga actgattgct
    11401 ttagaaggag attattgtga tattaaagta agaagagctt tagcaaatag taaggtatta
    11461 tcagctgatg tttctgctgg atatgaccca aattttggag aagcatatga aaagagaaat
    11521 tcggcatata tgggaaatgg agtagtttta actaagtata caggttcaag aggaaagagt
    11581 ggatgtaatg atgctaatgc tgaatttatg tcagaagtaa agggaatttt taataaagga
    11641 aatgtaatat ggcaaactgc tgaacttgga aaagtagacc aaggaggagg cggaactata
    11701 gcccatatat tggcaaatca aggtgcagag gttatagact gtggtgttgg agttttaaat
    11761 atgcatgcac cacatgagat tgtctctaaa gtagacatat atgaaatgta taagggatat
    11821 aaggcatttt ttaatataaa tttataagta aaaatataaa tgtaataagt gaataaaggc
    11881 tgttgtataa acaatttaat ctgttgtaca acagtcttat ttttatgtaa aaatagcaat
    11941 ttgtcacact agagaaaata aaagaaaata ctgtcaaatt aaaaattatt taaaaaaaaa
    12001 ttgacaacga tttcatcttg ttataatatg taataaagaa cttgttatat aatgttataa
    12061 aaatgttaca tagggaggtg agcaaaatgg atgaaaagag acaaaaaaaa gaaatggatt
    12121 tagaagtaaa ttcctttgtt cctttatatc aacaactata tgacaacata aaaaaacaaa
    12181 tagcatctgg catatataaa ccaggagata aacttccatc tgaaggagac ttatgtaaag
    12241 aatttaatat aagtcgtata actgtgagaa atacgacttt tttatataaa atagcaaaat
    12301 aacagtgttt gagcaaaaaa aagaatgttt tttatcgttt tgccacctgt ttgccaccat
    12361 aatttttttt cggtggcaaa ttattgtaaa ataccctcca aaatatctac tgtttcactt
    12421 tccattttac ttgtaacatg tgaatatgta tccatagtag ttgataattg actatgtcct
    12481 aatctttgtt gtatatattt tatattagct ccattttcta ataataatgt agcatgtgta
    12541 tgtcttaaac aatggaagtt gaagtcaagg tttaattttt tgttaattgt tctcacacaa
    12601 gcatcaatgt tgttatgatt tacaaatgag ccatcttttt ttctacaaac ccaatcatac
    12661 tcagtttctt tataccattt tccaatttta attttttgtt ttttttgatt caatttttcc
    12721 tcttttaata ttctagacaa agtatcacct attttaatat ctcttattga tgtttttgtc
    12781 ttaggtgatg ctaattcaaa ttctgaaact tttctcttta ttaaattttt tctaactttg
    12841 attatattat tatctaaatc aacattatcc caacaaagac ctaatatttc tccttttctc
    12901 atgcctgtat gaaaccctat aagtagagga atatagatat ttgtattttt aggatatatt
    12961 tctagtattt tattgaactc atctagtgtt atagtcttgt tatctgactc attttttacc
    13021 tttaaaacat tttttggtat actggcatat tgaacaggat tttccttaat gagtttgtaa
    13081 gggtaaacag cagattttaa agcagcattt aatacaacat aaatcgcttt taaaactcct
    13141 tttgtgtagt gtttttcttc tccgttttgt gtgtatgtct cttttgattt attatttaaa
    13201 aattcttgaa taatagctgg atttatagat tttagcttac atttaccaag tctaggttct
    13261 atatgatttt caattaaatt tctataactt tcctgagtat tgtatttgca attaagaagt
    13321 acatactctt tgtaccaaaa gttaagatag tctgataaac tcatatttgt ttcatcaaac
    13381 actataccag cgttttcata ttcatttatt gcatcacgga gcgatttttc agcttctttc
    13441 ttagtgtttc caccaactct ttctaccttt tttctcttgc catctactat acctgcatca
    13501 aagtagtaat accacttctt tccacgtttt cttacgccgc ctttcataaa atttctcctc
    13561 tcaaaagctt ttttatgtaa ttatataata acatatatca atttaaataa aataaataaa
    13621 aaagactata agaaacaaag ttttatgttt ataattaata gtctttttta tttagataca
    13681 tatttaatgt tttttatcac gtgattccag cactttttct gccaaataaa caactatttt
    13741 ttcatactca atatcagttt ctaaagaaaa gtcaacaata gcaggtatcg tcatatctcc
    13801 tgcatattta ttacctaacc aattttcaat taattccaat tattctttgc ttaattcaaa
    13861 ttttttcttt ttattaaaac ttaagttcaa tttattcacc tcaaataata ttatatatta
    13921 aaaatagtat ttgatagaaa cattcgaatt atttatgtaa ttttcttttt ttaatatatt
    13981 catcagcaaa ataatttact aaatattttt cttccaatcc taaagctatt tctaattcaa
    14041 taagaataga gatagttaaa tctttatcct cattattttc caacctagaa atttgacttc
    14101 tgtggcaacc aactcttttt gctaactcta tttgtgttaa tttcttcttt tttcgtaact
    14161 cttttaacat atatttaacc taccttttag ataattttat atatgttagt ttgtgtaatt
    14221 tcttgtaata aatgtgcaat attcgcacac tttttgttgg aaatttgtgc tagaatgtag
    14281 ttaagaaata actttatcta gataaagcaa aaataataga agctataaaa tataataata
    14341 ttttacagtt attttatagt tttaagcttt gaaaaagagg tggttgtaac tatttttaag
    14401 aacgtatgtt tcgtggaatt aataaataaa atattttata ggggatgata tatttgaata
    14461 agacgaaata ttatgatatt ttaaagttaa acttattaat gaaaaagttg aaagaattgg
    14521 ataaaaataa aatcaatgaa tataaaataa aagtgaaaga aatacataaa atcaataaaa
    14581 aagaggaatg attcctcttt tttattatat tcattttcag aaataaacta ttttttattt
    14641 ttttcgtcta gtaaaaataa ttctgcaact tttaatgctt tttctcttgc gtctgggctt
    14701 agctcgctaa atatattaaa aacttctttc atatcttcgt caagatacat attctcaata
    14761 agctcctttt ctgttttaaa attttcagca tcataatctt tttcgttttt atttataaat
    14821 aagctatttc taacatcagt tcttcctaat aaataatctg tagacacatc aaaatagttt
    14881 gcatattctt ctattgtact tttcttaggt tctcttaaac cattttctat ccttgatagt
    14941 gtagatttgt ttatatgtaa atcctcgctt aatttgtcta gcgagatgcc tttttcttct
    15001 ctcaattctt ttatcctatt caatttgcac aacccctttt tatattaatt ccaaaatagc
    15061 aacttttatt tatattatag caacaaaatt aattattaca attttttttg ctaaaaaagc
    15121 aacaaaagta ttgactataa tcacaaaact tgatattata taaatatgaa gttgccaaaa
    15181 tagcaaaaag gaagtgattt aatgtactta aatagattag aaggattgat gaaggaaaat
    15241 agacacaccc aaaaaaatgt ggcagatata ttaggtctta gctcatatgg ttttagatta
    15301 aaattaaaag gaaaaaacga atttaaagca agtgaaataa aaaagatatc taaattatat
    15361 aatgtatctg cggattattt tttttcagat gaagttgcta aaatagcaat aaaagaagaa
    15421 aggggcaata ataatgaaga atctaaccat aatcaagcaa aataatcaat ttttagttga
    15481 aagtagagaa gtagcagaat taatagaaaa gaagcacgat aatttattaa gagatataag
    15541 aggatacaag aagattttag aggactcatc aaatttgagg agtcaagatt tctttataga
    15601 aagtacttat ataaatactc aaaataaaat ccaaccttgc tacttattaa ctaaaaaagg
    15661 ttgcgatatg gtagctaata aaatgacagg agaaaaaggg attatattta cagcaattta
    15721 tgtaactaag tttgaagaaa tggagcaaga gttaaaagaa caacaaccta aattaccaac
    15781 tacatataaa gaagcactgc aacagttatt aatcgaagtt gaagaaaaag aacaactaca
    15841 attagaaaat caagaaaagg ataaggtaat ccagttacaa caaccaaaag tactgtttgc
    15901 tgattcggta gcgtcttctg acaattcaat cct
//
LOCUS       DAENCV010000085        15794 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAENCV010000085 DAENCV010000000
VERSION     DAENCV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.086"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            150..593
                     /locus_tag="KQD82_002680"
     CDS             150..593
                     /locus_tag="KQD82_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4381140.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            624..2441
                     /gene="ftsH"
                     /locus_tag="KQD82_002681"
     CDS             624..2441
                     /gene="ftsH"
                     /locus_tag="KQD82_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG4381141.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2619..5321
                     /locus_tag="KQD82_002682"
     CDS             2619..5321
                     /locus_tag="KQD82_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG4381142.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5355..6053
                     /locus_tag="KQD82_002683"
     CDS             5355..6053
                     /locus_tag="KQD82_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381143.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            6410..7366
                     /locus_tag="KQD82_002684"
     CDS             6410..7366
                     /locus_tag="KQD82_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG4381144.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            7543..7698
                     /locus_tag="KQD82_002685"
     CDS             7543..7698
                     /locus_tag="KQD82_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381145.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            7828..8841
                     /gene="aroF"
                     /locus_tag="KQD82_002686"
     CDS             7828..8841
                     /gene="aroF"
                     /locus_tag="KQD82_002686"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG4381146.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            8863..9927
                     /locus_tag="KQD82_002687"
     CDS             8863..9927
                     /locus_tag="KQD82_002687"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG4381147.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            9927..11240
                     /gene="aroA"
                     /locus_tag="KQD82_002688"
     CDS             9927..11240
                     /gene="aroA"
                     /locus_tag="KQD82_002688"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG4381148.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            11218..12297
                     /gene="aroC"
                     /locus_tag="KQD82_002689"
     CDS             11218..12297
                     /gene="aroC"
                     /locus_tag="KQD82_002689"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG4381149.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            12300..13496
                     /gene="pheA"
                     /locus_tag="KQD82_002690"
     CDS             12300..13496
                     /gene="pheA"
                     /locus_tag="KQD82_002690"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG4381150.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            13543..14352
                     /gene="aroE"
                     /locus_tag="KQD82_002691"
     CDS             13543..14352
                     /gene="aroE"
                     /locus_tag="KQD82_002691"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG4381151.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            14364..14897
                     /locus_tag="KQD82_002692"
     CDS             14364..14897
                     /locus_tag="KQD82_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG4381152.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            14918..15757
                     /locus_tag="KQD82_002693"
     CDS             14918..15757
                     /locus_tag="KQD82_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG4381153.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNKNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         6388 a         1629 c         2826 g         4951 t
ORIGIN      
        1 atttcaattg aagttacaat attgtaattg acaataaata cttaatacta taatatttaa
       61 tacattaaac attatagtgt taagtattta tttacagtat gcacttcagc ctaaatttga
      121 aataaagtat tttcgtaggg agcgtttgta tggatgagat gagttttaag aaagagctac
      181 tagagttgac tagagatata aatatgaaat ttacaacttt attaagtgat ttttatcagc
      241 cacttggtat tacagcagtg caagctttga ttttatctga gttatgtgaa catggagaaa
      301 aaaaaataag cgatttaggg aagaacttaa acatgacaaa tagtaatgta tcagtaatct
      361 gtcaaaggct tgaaaaaaat ggctttttaa atcgcataag agatatagaa gaccaacgaa
      421 ttgtaaaagt aaaagttaca aataaatcat tggatataca agaacatatt tcaagtagta
      481 tttttgatag ttattttgaa aatatgacca gtgaaaaatt gcaagatatg gaagatataa
      541 tagaaggttt agagaaatta aataaacttt taacttacat tgattgcaaa taacataaaa
      601 ataatttaaa ggagaataaa attatggatg aacataaacc ccaaaaaagg tcagcactct
      661 attattatac agttataata atagtagtaa tgtttttaaa tgctttaata tttccaagtt
      721 taatggataa aaaaatggtt gagattgact atggaaagtt tctcacaatg ttggatacag
      781 gaaaggttaa atctgttgag atagaaaata atagaatagc aataaatcca tctagtagca
      841 aggataagaa tatatacata actggtagaa tggatgaccc agaattggta gatagactaa
      901 aaaatgcaaa agtagaattt actaaggtta ttcctaagga aaattcacct cttttaagta
      961 ttttacttac atggataata cctataggta ttcttatgct atttggtaac ttaatgatga
     1021 agtctatgca aaagcgaatg ggtggtaatg ctatgcaatt tggaaaaagc aacgccaaaa
     1081 tgtatgtatc tgctcaaagt ggaaaaagat ttaatgatgt agctggtcaa gatgaggcaa
     1141 aagaagcttt aactgaaata gtagatttct tacacaatcc agaaaagtat aaaaagatag
     1201 gagcacaaat gcctaaagga gcacttttag taggacctcc aggtacagga aaaacactcc
     1261 ttgcaaaagc tgtagcaggt gaagcaaatg taccattttt ctcaatatct ggttctgagt
     1321 ttgttgagat gtttgtagga atgggagctt ctaggattcg tgatttattt aaacaagcaa
     1381 aagaaaaagc tccatgtata gtgtttatag atgagattga tactattggt aaaaagcgtg
     1441 ataatggaag tggattaggt ggaaatgatg aacgtgaaca aactttaaat cagttgttaa
     1501 gtgagatgga tggttttgat ggtggaattg gagtagtaat acttgctgct acaaatagac
     1561 ctgacagtct tgacaaagca ttacttcgtc caggaagatt tgatagacgt ataccagttg
     1621 aacttccaga ccttggagga cgtgaagcta tattaaaggt acatgctcaa aatgtaaatg
     1681 taagctcaga tatagattat aatgcaattg caaggtctac atcaggagca tcaggtgcag
     1741 aacttgcaaa tattgtaaac gaagcagcac ttttagcagt taagtctggc agagatatag
     1801 tagaacaaca ggattttgat gaatctgttg aaactgtaat tgctgggtat caacgtaaag
     1861 gagctgtaat ttcagataag gaaaagaaaa ttatagcata tcatgaaata ggtcatgcac
     1921 ttgttgctgc tatgcaaaaa cattctgcac ctgtccataa gattactata atacctagaa
     1981 cttcaggagc attgggatat actatgcaag ttgctgaaga tgagagtgta cttatgtcta
     2041 aagaagaagc aattgataaa ataactactt ttacaggtgg tcgtgctgct gaagaggtta
     2101 tctttaacac ttgtacatca ggagcatcaa atgatataga acaagctact aaaattgcaa
     2161 gagctatggt tactcgcctt ggtatgagtg aagagtttga tatggtcgct cttgaaactg
     2221 taagcaatca atatttagga ggagatactt ctcttgcttg ttcttccgac actgctgcta
     2281 aaatagatga agaggtattt aaaatagtaa aggcttgtca caaaaatgca attgaaatct
     2341 tagaaaataa tattgataaa ttgcatgaac ttgctaatta tttattagaa aaagaaacta
     2401 ttacaggtga tgagtttatg gaaatactta acttaaacta aaatgattat acacaagata
     2461 aaaatgccta ttgttaggca tttttatttt acaactaaat tttttaaatt catatttaaa
     2521 tctttacata atcttaatca ttaaaaatac atcttaacta caattttata tcggaattga
     2581 tattataata gtaatgaatg gtgtaaggag gttttttaat ggatagacct aatcctgata
     2641 ttctattaga aaaaattaaa aatgaagaag aaaaactaag tagaggtcaa ttaaaaatat
     2701 tctttggtta tgcagctgga gtaggaaaaa cctactctat gttggaatct gcacaaaatt
     2761 taaaaaaagt gggtgtagat gtagtagttg gatatattga accacacact agaccagaaa
     2821 cattggcttt acttgatggc ttggaaattc ttcctgtaaa ggagatagaa tataaatcta
     2881 taaaactaaa agaatttgat ttagatgctg ctttagcaag aaaaccagaa gttatattag
     2941 tagatgaatt tgcccattct aatgttgctg ggttaaggca tacaaaaaga tggcaagata
     3001 tagaagaatt gctgttagca ggcataaatg tctacactac tgtaaatgta caacatttag
     3061 aaagtctaaa tgacattgtt gagataatta caaacgtatc agttaaagag actataccag
     3121 ataagttttt ggatacaaat acacagttag aacttataga tgttgaacct gatgtacttc
     3181 ttgaaaggtt caatgaaggt aagatatata agaaagaaca agctactaga gcaaaaaata
     3241 atttttttat aaaagataac ctggttgcac ttagagaaat cgcacttaga aaaactgcag
     3301 agagagttaa taaagaagtt caaatgacca gactatcaaa gggtgatgta actgtaatac
     3361 ctacatcaga tacacttctt gcatgtataa gtccatctcc atcttcagca aaagttatta
     3421 gaactgcatc cagaatatca gacagtacat ttgcaaaatg gatagcttta tatgttgaaa
     3481 ctccaaacac agcaagactt ccatttgatg aacaaaaaca actacaaaat aacctaaaat
     3541 tagctaagaa attaggtgga gaaataattg ttttacatgg tgaaaatata atagagcaaa
     3601 tacttagaat tgcaaagctg agaaatgtaa caaaaattgt aataggtaga aatcattcaa
     3661 gtaataaaag atttagtaaa agatttaaaa aagatatagt tgacaaatta attgatgaag
     3721 ttgattatat agatatacat ataattccat ataaatttgc ctcagatgtt aaatacaaac
     3781 ctaaaaagga taaatcatca ataaaatcta aatttaaaat tagcaaagtt gattttataa
     3841 aacttatatt tattactttg atgataagta ttttagcata tgcagtacaa agcatgggat
     3901 ttataagaga aaatatattg ttaatatata tgttgggtgt agttttagtt tctatgtgga
     3961 ctaaagggta tagtacaggt ataataagtt ctgtatttaa tatagttctt ttaaattatt
     4021 tttttacagc accattatat acattatcta tagcagattc aaactatata gtaacactgg
     4081 ttgtattctc aatagtaggt ataataacta gcactcttac gtctaagata cagcatgaag
     4141 cagaaactgc tgctaaaaga gaagaaaata caaaaatgat atatcaaata attaagggat
     4201 ttttaagatt atcaaataaa gatgatatag taaacaaagg tatagaactt ttgtctctta
     4261 gcttaagtag agatgtaatt tgttatttaa atgtagataa gcataataaa tcaaagcttt
     4321 acaaaaaaaa tactaataat aaggataaaa atgatttgga tagtgaagat gaaaaagctg
     4381 ttgcaagttg ggttttatcc aattcaacag tggcaggaaa tgacacggat acacttcctg
     4441 gttcaaaggg atactatata ccaattgtag gaatgaattt aactttagga gtagtgggaa
     4501 tatcatgtat tgattcaaaa ttggatacag aagatataag cttaattgaa acaataattg
     4561 ctcaaatggc aatagcgctt gatagagaaa tcttgtcaga agctaaagaa aataccaatc
     4621 ttgagattga aagggaaaga ttaagaagta atcttttaag agcagtttcg catgacctta
     4681 gaactccact agcaggaata tctggtgcag taagtacaat tataaaaaac aagggcacta
     4741 taggtcaaga tataatagat gaattactta gtggtgtata tgaagatact cagtggttaa
     4801 taagacttgt tgaaaattta cttagtatga ccaagataga tgaagggaaa ttggaagtta
     4861 aaaaacattc tgaactggtt gaggaaatta tatcagaagc gttacaaaaa ataaaaaaaa
     4921 gaattgaaaa tgcacttata gatattaata taccagaaca gatattattt gttcctatgg
     4981 atgcaaaatt aatagaacaa gttttaatta atttaataga taattctttg aaatattcaa
     5041 aggaagattg taagatagag ataaatgtat atgagaaaga tgattatgtt tggtttgaag
     5101 tatctgataa tggtcctggt atatcaaagg aacttaaaaa gcatatcttt gacagatttt
     5161 ttactggaga agaaggagct aaagattcca gaaagggtgt tggacttgga ttatcaattt
     5221 gtaaatctat aattcaagct cataaaggtg aaattatggt agaaaacaat aaagataagg
     5281 gttcaacttt taagtttagt ttgcctaagg aaaatgaata gttttaaatt ttaaaattag
     5341 ttttggagga agaaatgagt aaaaaactta tattgttagt agaggatgat aagacaataa
     5401 gaaaatttat atctactgca cttttaacac aggattatga tgtcaaggag gctataactg
     5461 gaaaagaagg tatatctata gctgtctcat attctccaga tgttgttctt ttagatttag
     5521 gcttagaaga tatggatgga atagaggtaa taaaagctat cagacaattt tcaaatatac
     5581 caataattgt agtttcagct agagagcaag atagagacaa ggtagaagta tttgatgcag
     5641 gggcagatga ttacttaaca aaaccatttt ccatagtaga gttattagca agagtaagag
     5701 tagcatttag acattcacaa gtagaagctc aacaaaaaga tgatgttaaa tctacgtttg
     5761 aagtagataa attattgatt gattttgata aaagaaaagt tattgttgat gatgttgaag
     5821 tccatttgac acctattgaa tataatattc tttcattact tgcaaagcat catggaaaag
     5881 tgcttactca taattttata ataaaagaaa tatggggtag tgttataggg aatgagacta
     5941 agtctttaag ggtttttatg gcaacgttaa gacgaaaaat agaaaaacaa cctgcaaacc
     6001 caagatatat aattacagaa gttggtgtag gatacagatt aaatgatgaa tagttgtaat
     6061 atattaatta ttagattatg tgcttattaa tatatattaa aattatatag tttagttaaa
     6121 ataagaatga agtgtatgtg cttgttgtca gtttagcaca tgcattttat tttttagctt
     6181 ttttagtatc aataattttt tagtatcaat aatatgaaat atgttttaaa ataagtatat
     6241 agtgtaatct gtaataaaaa ctttggttaa ataataaatg tatttaacac ttaccttata
     6301 ataaatttaa tataaatatt aaataataat tattaaaata gtgtaaaata attacagtaa
     6361 ggattataaa aaatttttag aatgtaaatt gaaatttagg agtagatgta tgtatatact
     6421 gaatttaaat acaagagaga ctattgaaga ttttagagat aaattttatg tagcagaaaa
     6481 ttcttatctt atattaagtg ctccaaaaaa tttaaaattg cttaaggaaa ctttggatat
     6541 agatgaaata acttttaatg attgtcttaa atttgatgaa ataaccaaac ttgatttatt
     6601 tgataattac gattttttaa gcctaaatac atttgaacta agagatggag aagctgttat
     6661 agaagaggta aatatgtatt tatcagataa ttttatattg gttgtagtga atgaagaaca
     6721 ctttttattt gaatttgtaa aaaatattat tttgaagaac tctcaacttg aaaaaaatcc
     6781 agttattaat ctatttaaga taaactactt gattcttaga gaagttataa aaaatggttt
     6841 tgagtctttg gaaaaagttg aagagttaat tcttcaaata gaagatgaaa tgatggacaa
     6901 cattaataag aatcatgtat ctagaataag tgatgtaaga ggattaacaa gaattatagt
     6961 taagaatact agacctcttt tgtatattgg ggatagaata gttaaggaaa atataagata
     7021 tttaaaatat tcaaatgtaa aaaaatataa tttagaaaat tttcaaggta tagattttgg
     7081 aattgacaaa ttatacaatt tcgcattgtc aacacgagag ttagcagata aattattaga
     7141 tatttattct tccagagtag gtgagaaaac aaataactta attacaaaat taaccttgtt
     7201 aacagccata tcagcaccac ttacaattat aactggtata tatggaatga actttagata
     7261 tatgccagaa ttaaactgga tttatggata tccagcaact ttatttttta tgctatgtat
     7321 aatttttgtt ggaattataa tatttaaaat aaaaaaatta ttgtaaatta cagatgatgt
     7381 ttaattgatt ttataaaaat acatcaattg agaagctgat ttaagtaaat aaaaaaatat
     7441 tttaaattga aaatatttat agaaaatatt gacaattata ttttctttgt tataattatt
     7501 aaacaagaag aataataaat tataaaaatg gaggataata ctatgaaagg aatattaaaa
     7561 gaactaacag caaaacttta tagctttttt tactttatgt tctggggctt ttattttagc
     7621 gctggctatt tcttttacaa aaatatatgt acttcctttc tggtgaggcc ttattttagt
     7681 gattttgagc ataagtaaat tttataaaaa tgattatttt atatatttat ataagattta
     7741 gtgcttgata gattatttta gagaactcat tagtgagttc tcttttttat tttaaatata
     7801 aaattataat attgggaggt agtaaaaatg atagtagtat taaaaatggg agcagataaa
     7861 aatgaagtta aaaaattaat tgaggctatt gggagagaag gagtagaagt taatcccata
     7921 gatggaacag aattaactgt tttaggttta gttggagata ctagtaaaat agatgccaaa
     7981 agaatagaag caaataaggt agttgaaaaa gttatgcatg tggtagagcc ttttaaaaag
     8041 gccaatagaa aatttcatcc agaaccttca atcataaatg ttaatggaat ggaaattggt
     8101 tcaaagaaaa ttgctatgat agctgggcct tgttctgttg aaacagaaga ccaaatagta
     8161 tctatagcta aagatgtgaa aaaatcaggt gcaggatttt tgagaggtgg agcattcaaa
     8221 ccaagaacat caccatacgc tttccaagga ttgaaatatg atggattgga tttattaaaa
     8281 aaagcaaaag aaaaaacagg gcttcctata gttacagaaa taatgtcaac acaagatata
     8341 gatatatttg aagaaaatgt agatgtaatt caagttggag ctagaaacat gcaaaacttt
     8401 gatttattaa aagaattagg taaaacaaat aaaacaatac ttttaaaaag aggattatct
     8461 gctacaattg aagagtggct aatgtcagca gagtatataa tggctggagg aaatgaaaat
     8521 gtagttcttt gtgaaagagg tataagaact tttgaaacat atactagaaa tactttggat
     8581 ttaagtgcaa ttttagctgt gaagaaactt agtcatttac cagttatagt tgacccaagt
     8641 catgcagcag gtaaatcatg gatggttgac tctttatcta aggcagcaat tgcagttgga
     8701 gcagatggtc taataatcga agttcataat gacccagcac atgcactttg tgatggaaaa
     8761 caatcgatta agccaaatga atatgatgaa ttgataagtg aattaaagac tatcgcatca
     8821 gcagttggta gagaaatata atctataaaa ggatggttaa acgtggaaga aaaactatta
     8881 gtaaatttaa aagaaaatag ctacaatata tttattaaaa aaaatatact aaagaatata
     8941 ggagaagaaa taaaaaaaat atattttgga aataagattt ttattgtaac tgatgaaaat
     9001 atagcaaaat tatacttgga taaagtaaaa aataatttaa gtgaatctgg atttgaaatt
     9061 aaatctttaa tattaaaatc tggtgaagaa acaaaatcat ttaatactct tcctaaaata
     9121 tacgatagtt tacttgactt taagttaact agaaaagatt tgattattac tttaggagga
     9181 ggggtaatag gtgatttagg tggatttgca gcagcaactt ttttaagagg tataagtttt
     9241 attcaaattc caacatcttt attagcacaa gttgatagtt ctgtaggtgg aaaagttgga
     9301 gttgaccttg agagaggcaa aaacttagtg ggaagctttt accaaccaaa ggctgtgttt
     9361 atcgacccaa atgtattatt atcacttcca gaaaaattct atagagatgg aatggcagaa
     9421 gttataaaat atggatgtat aaaagacaaa acttttttca ataaactttt aagtttaaaa
     9481 aatagagaag aagtaatgca acatatagaa tacattatac ataaatgttg ctttattaaa
     9541 aaaactgtag ttgaagctga tgaaaaagac tttggagata gaatgcttct aaactttggc
     9601 catacattag gtcatgctat tgagaaatac tacaatttta cgggttatac ccatggagaa
     9661 gctgttgcca taggaatgta taagataact ttagaaagtg aaaagatagg aattacaaaa
     9721 aaagggacat cagaacttat aaaatcaatt ttaatgaatt ataatttgcc ttatgagatt
     9781 gaattaagag ataataaatc tatagtagat gctatatctc ttgataaaaa aaatctaggt
     9841 gatacattaa aaataatact tttaaaagat attggagaga gtattatata taatactact
     9901 tctaaatttt ttcaggaggt aagataatgg gaagtctaaa aatatatcca agtaaattat
     9961 ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca tagggcagtt atttgttcat
    10021 ctctaagcaa tggaaaaagt agaatttcta atatagattt ttctgatgat ataatagcta
    10081 ctataagagc aatgacatca ttaggagcta ttatagaaaa gaaagaagat attttagaaa
    10141 tttcgggaat tttttctaaa gaaggtattt taaatagaga gaatcagttg aatcaaccta
    10201 aattgacaat agattgtaat gaatcaggct ctacattgag atttttagta cctatatctt
    10261 tagcatttga tggtgtaaaa aggtttatag gtagaggaaa tcttggaaaa agaccacttg
    10321 atacttatta tgagatattt gacagacaga atataaaata tagttacaaa gaaaatcagt
    10381 tagatttgat tatatcaggt aagcttaaac cagatgaatt tagagtaaaa ggaaatataa
    10441 gttctcagtt tatcacaggt ttactgttta tattgcctac gctagagagt gattcaaaga
    10501 taataattac tactgagcta gaatctaaag gatatttaga tttaactctt tcaacgataa
    10561 aagattttgg ggtagaaatt ataaacaata actataaaga atttattata aaaggaaatc
    10621 aaacgtataa agcgagagat tataaagtag aaggagatta ttctcaagga gcattttatt
    10681 tatcagcaga tgctatagga gaagatatta gcatattaga ccttaaagaa gattctcttc
    10741 aaggggatag tgaagtagtt gaaattttat ctaggatggg aatggaaata ttaagagaag
    10801 gcaacaaaat aaaaggaata acaaatggtt taaatggtac tttgatagat gcatctcaat
    10861 gcccagatat aatacctgta ttatctgtag tagcatcctt aagtataggg aggactacaa
    10921 taattaatgc tggtaggctt agaattaaag aatgtgatag acttcatgca ataaatgttg
    10981 agttaaataa acttggagca aatatagagg agaaagagga tagtttgata atcgaaggag
    11041 taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa agaccataga attgctatga
    11101 cacttgcaat tgcttcttgt agatgtgata aaccaataat attaaaagat tttgagtgtg
    11161 tatcaaaatc ttatccacat ttttttaaag attttaagat gttaggaggt cgaattgatg
    11221 agtggaatat ggggaaataa tttaaaagta tcaatttttg gagaatctca tggaaatgct
    11281 ataggtataa atatagatgg tcttccttcg gggattgaac ttgaccttga taatattgat
    11341 aaagaaatga agagaagagc acctggaaaa aattctatat ctacttcaag aaatgaaagt
    11401 gatataccag aaattctaag tggatatttt aatggaagaa ctacaggaac gccattatgt
    11461 gcaatcataa gaaatagtga tactcgttca aaagattatg gagagcttaa aaacttaatg
    11521 agaccaggac atgcagattt cacaggtaat gtgagatatt ctggatttaa tgactataga
    11581 ggtggaggtc atttttcagg taggataaca gctccattag tattttgtgg ggctatatgt
    11641 aaacaaattt tatcacaaaa aggaatagag ataggagcac acataaaaaa aattaaaaat
    11701 atagaagata tgagttttga ttatgtaaat atttcaaaac aacagttatc aaatttgcaa
    11761 actttggaac tacctttact tgatttatca aaggaagaag ccatgaaaaa tacgattata
    11821 gatgcaaaaa atcaaggaga ttcagttggt ggtataattg agtgtgcagt agtaggtatt
    11881 aatgtagggc ttggaaatcc attttttgac tctgttgaat ctactttatc acatttattg
    11941 ttttctgttc ctgctgtaaa aggtgtagag tttggtttag gttttgaact tgcagatatg
    12001 tatggctctc aaagtaatga tgaaatgtat tatgaaggaa atcaagttaa gagcaaaaca
    12061 aataacaatg gaggaattat aggagggatt actactggaa tgccaataat tttcaaagtg
    12121 gcaattaagc caactccatc aattagcaga caacaaaaca cagttaatat aaaagataaa
    12181 aaggacgata ttttatatat taaaggcaga catgaccctt gtatagttca aagggctata
    12241 cctgtaatag aagcagtaac agccattgga atttttgatt tgatgaaagg acgataataa
    12301 tggaggattt aacgagatat agaaatgaga ttgacaatat agataaagag cttattcaat
    12361 tatttgaaaa gagaatgaat acagttcttg aaattgccag acataaaatg aaaaataaca
    12421 ctactatact tcaaaaagat agagaagaaa aagtactaag tagagccgta gataatttag
    12481 ataataaagc atattcacaa gaaacggtac agttttttaa ttctataatg gaaattagta
    12541 gaggattaca aaaaagattt atagataatg atggtgagca gaaaatcgag tttagtttag
    12601 attcatcaaa taagaatttt gatttgtcaa atgtaaataa atataagtct ttaaaagatg
    12661 aattaaataa aaaaaatatc ctagtaggct ttcctggaaa atcaggttca tttacagaag
    12721 aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa acaatttaaa gaatttgaag
    12781 atgttttcat agcacttaaa aataaagaaa taaactatgg aataatcccc atagaaaatt
    12841 catctacagg agctatctct gaaacttatg accttttgag aaagtacggt ttttatatag
    12901 ttggagaaga atgtataaaa atagaccaaa atttaatagg gataaaagac acaaagcttg
    12961 aagatataaa agaaatatat tctcatccac aaggtatagg acaatgtagt gaatttttaa
    13021 aacaaaatag tacatggaaa ttgataccat ttaataacac agcaacaagt gcagaacttg
    13081 taagaaaact tcaagataaa acaaaagcag caattgcaag caaaaaagct gcaagtattt
    13141 atggattaga aataataagt ccttgtataa atgatatagc aaacaactat acaagatttg
    13201 tagtaatcag taatcaaata cacattgaag aagaatcaaa caaaatgagt gtagtatttt
    13261 ctgttgaaca tgaagcagga aaactttata aagtacttgg atactttgct aaaaataata
    13321 taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc ctcatggaga tatttctttt
    13381 atatagattt tgagtgttct atatacaatg ctaaagtgta cgatttatta gaattgatag
    13441 aacataatac agcatatttt aaatttatgg gagtatataa aaataaaact gaatagataa
    13501 gataaatttt tatttaattt ataattatta ggaggtgtaa gtatgaattt tttcggtcta
    13561 gtaggtgaaa aactctctca cagtgtttca cctcaaatcc acaagagagt attcgagata
    13621 ttgaatattg aaagtgctta taaaaacttt gaaatttcaa aagaagatat atctaagcta
    13681 gacggtgcta taaaattatt aggaatacaa ggggtaaatg ttactgtacc atataaggag
    13741 cgtattatga aatatcttga ttttatatca ccagaggcaa aacgtatagg agcagttaat
    13801 acaattttac tcagagaaaa tatgttatat ggttacaata cagattactt tggtcttgat
    13861 agtatgttta aaatggctaa tattgatgtt caaggaaaag tagcagttat attaggaact
    13921 ggaggtgctt ctaaagctgc tcttacatat tttattgatt ctggtataga aaagctttat
    13981 gtttcaacta gaaaaaagga tgataaaaaa ttattaaata gtaaagctat attaatagat
    14041 tatgaagaac ttaaacatat aaaaggagat ataatattaa atgctacacc agtaggtatg
    14101 taccctaatg taggaatttc accagtgtca aaatctatta tacaaaattt tgatatatta
    14161 atagatttaa tttacaatcc tggagaaaca gagtttttaa gaattggtaa tagtatgggt
    14221 aaaaaaactt gtgatggatt atatatgctg gtggggcaag caataaaatc tcaggaaatt
    14281 tggcaagata ctaaaataga taatagtatt ttagatgtta tatataatga actaaaatta
    14341 gaatttttgt aagggagatt gttatgataa ataaaacaaa agaaaaattg atattaatag
    14401 gtatgccagg tagtggaaaa actacaattg gtaaactttt ggccaaagaa tataattgta
    14461 gtttctgtga tatggatgat tatataattc aaatatctca aaaaagtata gcagagttgt
    14521 tttcagaagg agaagatatc tttaggaact atgagactca agcatgtaga gaactttcta
    14581 taagtgataa aacagtgata tctacaggtg gaggtgtaat aaaaaaggat gttaatatgg
    14641 aaatactaaa agaaacaggt ataataattt ttattgatag acctatacaa aagattttgg
    14701 aagatataaa tattaattca agaccattat taaaaaacgg aaaagataga ttgtataatc
    14761 tttataatga gagaataaat ctttataaaa aattttcaga tatagagatt ttaaatgata
    14821 aatcattaaa taatgctgta tataatatta caaatgcagt cagtgaaaat tttaaatttg
    14881 actttaaaga aaaataatct agggggaagt aaaaataatg aatattgtta ttgtaggact
    14941 aggagttata ggaggctctt ttgctaaagc attaaaaaaa gctggatatg aaaatgtatt
    15001 tggagtagat gtagatttag aaactttaaa aaaagcagaa aaagcaaaaa tcataaaaaa
    15061 aggatgtact acaggtaaag agttatttaa aaaagctgat ttaattatac tatctatata
    15121 tcctagatta gttgtagatt ttctaaataa gaataaaaac ttttttaaaa aagggactat
    15181 aattactgat actacaggaa taaaagaaac acttatcaat gatgtgttgc aaattattcc
    15241 agatgatata gattttatat ttggacatcc aatggcaggt agggaaaaaa agggtattga
    15301 ttttgcaagt gaacaagttt ttaatggtgc aaattatata attactccaa ctggcagaaa
    15361 caacataaaa aatttagagt tagttgaaaa tttaatttta gagattggat ttaaaagagt
    15421 aaaaaagtta acttcacaaa aacatgatga gattatagct tttacttctc agttacctca
    15481 tgttatggca gttgctctta taaatagtga tgaagaaggt agagatacgg gtaaatttat
    15541 tggagatagt tatagagatt taactagaat tgcaaatatg aatgaagact tatggagtga
    15601 acttttttta ggaaatagag ataatttact aaaagttata gaaaactttg aatcagaagt
    15661 aaatttagta aaagaagcta tatttaacaa tgataaaaat aaattaatag agtattttaa
    15721 aaaatcatca ataagaagag aagctttaga aaaataaata ttaatattta agctatatat
    15781 agatgggtat aaat
//
LOCUS       DAENCV010000086        15688 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAENCV010000086 DAENCV010000000
VERSION     DAENCV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.087"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(60..677)
                     /locus_tag="KQD82_002694"
     CDS             complement(60..677)
                     /locus_tag="KQD82_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG4381154.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(804..1826)
                     /gene="argF"
                     /locus_tag="KQD82_002695"
     CDS             complement(804..1826)
                     /gene="argF"
                     /locus_tag="KQD82_002695"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG4381155.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(1935..3122)
                     /locus_tag="KQD82_002696"
     CDS             complement(1935..3122)
                     /locus_tag="KQD82_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG4381156.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLQIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(3152..4012)
                     /gene="argB"
                     /locus_tag="KQD82_002697"
     CDS             complement(3152..4012)
                     /gene="argB"
                     /locus_tag="KQD82_002697"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG4381157.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(4027..5244)
                     /gene="argJ"
                     /locus_tag="KQD82_002698"
     CDS             complement(4027..5244)
                     /gene="argJ"
                     /locus_tag="KQD82_002698"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG4381158.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(5285..6319)
                     /locus_tag="KQD82_002699"
     CDS             complement(5285..6319)
                     /locus_tag="KQD82_002699"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG4381159.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVRAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(6573..6786)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7090..8181)
                     /gene="ytvI"
                     /locus_tag="KQD82_002700"
     CDS             complement(7090..8181)
                     /gene="ytvI"
                     /locus_tag="KQD82_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG4381160.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWGIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGILLLIAFLKKLNDKEIIHILH"
     gene            complement(8196..9587)
                     /locus_tag="KQD82_002701"
     CDS             complement(8196..9587)
                     /locus_tag="KQD82_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBG4381161.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFADYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVETRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            complement(9606..10145)
                     /locus_tag="KQD82_002702"
     CDS             complement(9606..10145)
                     /locus_tag="KQD82_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381162.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREISFHLWKK"
     gene            complement(10166..11491)
                     /locus_tag="KQD82_002703"
     CDS             complement(10166..11491)
                     /locus_tag="KQD82_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381163.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            complement(11488..12111)
                     /locus_tag="KQD82_002704"
     CDS             complement(11488..12111)
                     /locus_tag="KQD82_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG4381164.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            12203..13012
                     /locus_tag="KQD82_002705"
     CDS             12203..13012
                     /locus_tag="KQD82_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381165.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            13446..13808
                     /locus_tag="KQD82_002706"
     CDS             13446..13808
                     /locus_tag="KQD82_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381166.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(14158..14715)
                     /locus_tag="KQD82_002707"
     CDS             complement(14158..14715)
                     /locus_tag="KQD82_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381167.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQL
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            14973..15671
                     /locus_tag="KQD82_002708"
     CDS             14973..15671
                     /locus_tag="KQD82_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381168.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELISIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPAKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         5301 a         2790 c         1758 g         5839 t
ORIGIN      
        1 ttatatgttt atttataaaa ataatcatat atttattact ttactacacc taaacaatat
       61 tattcaagtg tattcataga gttagaaaat tcttgtcctg ctgtaacaag attagataac
      121 aatacatcag ctactgcatc atttactgaa cttattatcc atttattatc ctttttagaa
      181 cattcaaact ctacattttt tgtaacataa gtctctttaa cgcttttttg tttttctttc
      241 ataattgata ctaacatttc agttgttttc tcatcagtta agtcttgtcc tgaaaaactc
      301 ataccaatca tctttgtaaa tgcctcagct actatttctt gaagtaatgg tgttgaatct
      361 ataaatttac attctacttt gattgtagcc ttatcacctt taacctcgct gtcttttatt
      421 gtatatgtga ttttagatgc attctgtttt agataatcta aaacatattg ggcatttggg
      481 tcttttgtat ctaattcctt taatatatcc ttatattttt cgtcattatt ttccattacc
      541 ttattcatgc cttcaaagtc aaactttttc gcactgttaa aaaaactatc aattgtatct
      601 tctggttttg cacttgaaca tgctgttaaa gatattgcta ataaaaaagt tgttaaagct
      661 aatatgattt ttttcatatc tcaaatcctc cctcttaatt tgttgataca taagttattt
      721 taacatataa tgagagtttt tgaataatat atttaaaata ttttataaat tttcattaaa
      781 gtaaagtcta tattaaaaca tatctatatt aaattattgt tcatatttac atcagtcata
      841 gtagaaacca taacagcttt tattgtatgc attctatttt cagcttcttc aaatactttt
      901 gaatattttg aattaaatac ctcgtttgta acctctaatt ctgataatcc gaacttttca
      961 taaatctcct gagcaacctt agtttctaaa tcatgaaatg ctggaagaca gtgtaaaaat
     1021 ataacatttt cattttcagt tttctttatc atatccatat ttacttgata atctttaagt
     1081 tgatttattc ttgcttcata ttgctcctct tctcccatag aaacccatac atctgtatat
     1141 ataacatctg cacctttaac atcatcaata ttttcagtca acattatatc tcctttggat
     1201 ttcttggcta tttccttcat ttccttaacc aattcttcat ttggccaaag ttccttaggt
     1261 gctagtgcta cgaaatgtat tcccatttta gcacatccta tcatcagtga gtttcccata
     1321 ttatttctag catcaccaac atatacaaat ttaactttat tcaaatcttt atctacattt
     1381 tcaattactg ttaaaaaatc tgccagtatt tgtgttggat gatattcatc tgtaagtcca
     1441 ttccaaacag gtacaccaga atattttgct agactttcaa ctgtttcttg tttaaagcct
     1501 ctaaattcta ttccatcata gaatcttcct agaacttttg ctgtatctga tacagactct
     1561 tttttaccca tatgacaatc atttggacct agataagtta catgtgctcc ttcatcatga
     1621 gctgctactt caaatgaaca tctagttctt gttgatgttt tctcaaataa taaagctata
     1681 ttttttcctt gtagcaaatc gccttttata ccaacttttt tcttagtttt taaataactt
     1741 gataaagtta ataaatatct aatctcttct tttgtaaaat cctttaatgt caaaaaactc
     1801 ttacctttta aacttatatt attcatatta attcgcccct tcttataaac tttttaaatt
     1861 caattaaact tgttagcact ttactttttt atttacttct aatttttatc tttaatcaac
     1921 tatttcttga atatttagtt aatttctagt aatatatctt ttaggatttc aagagcctca
     1981 tctatttcct tataactgat tgttaatggt ggtaaaaatc tcaatacatt ttttccagca
     2041 gtcaaaataa gcaatccttt ttttctagct ttctcttcta taatactaga ttcaatatta
     2101 gtttcaattc ctatcattag accaatacct tttgtcttta atacaacttt gttattaaaa
     2161 ctttcaatag attttcttat ataatttcct ttttctaata cttcattata aaaaccatct
     2221 ttagatatct catctaatac aactgaagct gccaccatag ctaataaatt acctccaaaa
     2281 gttgtacctt ggtctccttt tcctagtact tttgaaagtt ttttatttac caatactcct
     2341 cctactggta ttcctgctcc caaccctttt gctacggtaa ctatatctgg ttttacatca
     2401 aaatattcat agccaaaaag tttaccagtt cttcctatac cgcattggac ttcatcaaaa
     2461 atcactacaa tatccttttc ttgacatatt tttactattt gcaaaacata atctttttca
     2521 agaacattaa ctccaccttc gccttgaatc gcttcaagaa ttattgcaca aactgtggaa
     2581 tcaagattat tttttaaatc ttcaatatta tttctttcta tatatttaaa accttcggga
     2641 agaggataaa aatacttatg gtatttgtcc ataccagttg ccattaaaga catcattgtt
     2701 ctaccatgaa aagagtcttt taaacttatg atagttcctc taccattacc atatttgtca
     2761 ctactatact ttcttgctat tttgaatgct gtttcatttg cttctgttcc agaattacaa
     2821 aaatatactt tagccattcc acttgaatta attattttct ctcctgaata caacactggc
     2881 tcattaaaat ataaatttga agtatgtaat aatttatcta cttgctcttt tagagcattc
     2941 tttaaattac tattactata cccaagagaa gatactccat atccacttgt aaaatctatg
     3001 tatttattgc cattagtatc aaaaaaacaa cttccttcac cataatctat gacaaaattg
     3061 ggttggttat atgtatctat aaaatattca ttccattttg aaattgtatt gtcgttattc
     3121 attcttattc tcccctctta aacttataat tttattcatt ttcttttctt attaatgtac
     3181 ctattccact atcagtaaat agctctgtta ttatagaatg tggaactctt ccatccaaaa
     3241 tatgtaccct atttactccg ttatttaagg catctacgca tccctctatt ttaggtatca
     3301 tacctccagt aattatacct tcttcaaata atttatcaac atcctctaat ataacttctg
     3361 ttattaatga tttttcttca tctggctctc ttaacaaacc aggtacatct gtaagaagta
     3421 tcaatttatc tgcatttaac tctttagcta tagcagaagc tgctgtgtca ccatttatat
     3481 tataagtctc tccatcatct ccaacaccta tagttgcaat tactgaaata taacctgact
     3541 ttagacaaga ttctataagc tcaacattta cttttttgat ttctcctaca aatccaagtt
     3601 cataattttt atatgggtca cataaaatca tattattatc tattccacaa agccctactg
     3661 cttttccacc ttttgtatgt attttattta ctaggtcttt attaaccttt ccagcaagta
     3721 ccatttttac aatttccatg gtctcttcat ctgtatatct aagaccattt acaaatttac
     3781 tttctatatc cacttttgct agcatcttat tgatttctgc ccctccacca tgtactaaaa
     3841 ctatattgat tccaacataa ctcattaaca caaggtcttc cataacagat tcttttagac
     3901 catctttttt cattgcactt ccaccatact taactacaat agtctttccc tgatgttttt
     3961 ctatatatgg taaagcttct ataagtgtat ttgctttttc tatattaatc attaaagtat
     4021 atcctcctaa tttctataac ttccatttat tcttacataa tcatagctta aatcacatcc
     4081 ccatgcattt cctctggcat tgcccatatt taaatctact aatatactta tttcgtcatc
     4141 ttcaaggatt ttttttgctt tttcctcatt aaaatctaat ccatttccat ttttacatac
     4201 ttcaatatat cctttcatac tctcaaaaat aatatcaact ttttccatat ctatttcttc
     4261 acctgcataa cctaaagcac acagtattct accccaattt gcgtcagctc caaatacagc
     4321 tgtctttact aatgaagaag atataactgt ttttgctaat tttactgcat gtgcttcact
     4381 taatgctcca tttatatagc attcaattag ttttgtacat ccttctccat cttttgcaac
     4441 aagtttagca agttctatac agacatatgt aagtgcactc aaaaatactt gataatgttc
     4501 atcttttttg tttattctat cattttttgc ttttccattt gctagaatca ttaccatatc
     4561 attagtactt gtatctccat ctacactaac tctattaaaa cttttgtcaa cagcaatttt
     4621 taaagcttct tttagcaaaa ctccatcaat atttgcatct gtagttataa atcctaacgt
     4681 tgttgccata ttaggatgta tcataccaga accttttgcc atacctccaa tagttatttt
     4741 tttatctcca tacattattg ctactgctat ttcttttttt ataatatcag ttgtcataat
     4801 agcttctctt gctccaatat gaccttgttt acttagatta ttcactaatt catccatatt
     4861 ttcttctatt aaatctatat ttaatggctt tcctataaca cctgtagatg caaccaaaac
     4921 atcactttct ttaatattta atttatcact ggtgaattta gccattttat aagcatttat
     4981 aagtccttct tttccattaa aggtattagc attagcacta tttactatga tagcttgtgc
     5041 ttttccattt ttaatgtgtt cctgtgtaac ataaacagga ttgcccttta ctttattttg
     5101 agttactact gctgctgcat cacataaaac atctgaataa accaaagcta aatctctttt
     5161 ttctttattt tttctcaagc cacagtgaat ccctgaagca aaaaatcctt ctgaaactgt
     5221 aactccaccc tttataatct ccatatttat cctcctaatt atatgagttt agtatttttt
     5281 atttctaaaa tgatggtgca atatttttta aacctgtatt ttcttcaata ccaaatatta
     5341 tattcatgtt ttgtatagct tgacctgccg ctcccttaat catattatct atagctgaac
     5401 agattatcaa tgtatctcca ttttcatgta atgatattac acatttattt gagaatctaa
     5461 cattctttaa agaagctatc ttatctaatg gaagaatttc tataaattct ttaaattcat
     5521 aataatcagt taatttccta tgtatatcat ttatagaaat attatcttct tttgtacaat
     5581 atatagttga taagattccc ctatttactg ggattaagtt tggtgtaaaa actacactta
     5641 ctttttcttt gcaggattca cttaaatttt gttctatttc tggagtatgt ctgtgtttcc
     5701 ctattttata tgcagtgata ttttcattaa cctccgtaaa gtgactacta atcgataatt
     5761 ctctaccagc acctgtaaga cctgatttag aatcaattat tatattatta ttttttatca
     5821 atttagaact tagtaatggc atcaatggta aagaaatact agttggataa catccaggat
     5881 ttgcaattat atctgcatct tttatatctt ctttgtatat ttcacttaat ccatatacag
     5941 cttttttatg taagatttta tctataaaag atactccata ccatttacta tatacttctt
     6001 catcttttat tctaaaatca gcacctaaat ctataacttt ctttttcgat ttaattgcct
     6061 ttattacaaa cttttcacta actccatgtg gaagggctgt aaacacaaca tcacacatat
     6121 ctataacttt ttcattttct atacatatca tgttattttt atatcctata cttggatata
     6181 tatcaactat atcttttcca acatacgaat ttgaacctat cgctctcact tcgactttat
     6241 catgattcat taataatcta atcaactctg cccctacata tccagttgca ccaataatac
     6301 caacttttat tttattcatt ttgttccctc catttttata ttcaaatagt cttaaaatcc
     6361 tatctttcca ctttactata tgataatcat ttgataatac gtagattaaa atactctaat
     6421 taaaacaaaa aaccccttag cattataaat actaagaggc gattttccgc ggtaccactc
     6481 ttattggtaa aattacaata atcattaaaa ctcaaattaa aaagtatcta caaaatgtac
     6541 atttaaaatt aaaaaagcct cttggtcaaa gaccaagaga cgaatataca cccgcgttac
     6601 cactctaatt gataagataa tcttacccac tctaatctct taaggcgaga ttcaccaatt
     6661 accatactat tacttctggt aatctcctcc aaagctctat tcacaataaa attcgttact
     6721 aggcttccac cattcccagc tctctatcaa ctagctttat gctactctct ttttcacagg
     6781 atttcaattt attttcttat tgttttatat gatagtttat ttttttaact ttgtcaatat
     6841 aatttttaaa tttttggaaa tattttctat ataattaatt tccagcattt tgtatctaaa
     6901 ttgagttata aatatctaca gtaataggac accatgaatt tcctgctgta atatatttta
     6961 aaaaagttct ccctcttgaa tcaagtcatt caaaagctat accttatgtc aaaattattt
     7021 ttcatccagc gttttttaga aaaaacacaa ttttctccat aaaagagaca tacatacaac
     7081 tattctttcc taatgtaata tatgtatgat ttctttgtca tttaactttt tcaaaaacgc
     7141 aattaacaat agaataccga acaacccaat aaatccaaag aaatgtaatc ctgttagcat
     7201 actggctaat gccaatacag gatgtaactc catatgttta ccaactaatt ttggttcaat
     7261 aatattcctg ataatagtaa tcacaatata tagtagtgca attcctaatg ccattgaata
     7321 atcccctaaa aataaactga taatacccca aggtattaga atacttccag ttcctaaaac
     7381 aggtagtata tctaaaagag caatgaataa agcaattatt ccagcataag aaatattaat
     7441 aatagcaaaa ccgacatata attcagaaaa agtgatagca aaaattaaaa gataagaaaa
     7501 accacattta ataatggtat caatagtaaa cgcttttgct tctgctaaaa gataactttt
     7561 atttttaggt atctgtagca ctataaatct ctttatcgtt ggataatcaa ttgctataaa
     7621 aatagaagca acgattgtaa taattgtttt cataaatagt aatggaacag acgaaacaat
     7681 atcagtaata ccagatacga tattatttga aagagaaact atgaaaacac tcatttcatt
     7741 catcaataca ggcatatttg cttctattaa ttcaataaac gaaaaattga atgaaccaga
     7801 aaatagttcg acttctttta taactgtttc agtcaaaggt attaccaatt cctcaaacat
     7861 ggttggtagg aaatgaaaaa tttcctttat tccatatatt agatatgtac ctattacact
     7921 tactattatt cctgcaatta aaaagaataa aaaaacagat atggttgcta caatacctcg
     7981 ctttattagt aatttttcac ttatgtataa gactggttta ttcaataaac ttgctattaa
     8041 aaatgcaaaa acaaaaggaa gtaatacaga aaacgcatat ttccctaata acattagtaa
     8101 gcctaatatc actaccaaat aacagaaatt tattaaaaat ctctgcttat tttggtatga
     8161 tgtatgatct tgttcattca aataaaatgc acctcctact ttatagtaac tttatatgtg
     8221 ccttttggag caacaggagg cattgtttca cacattccag aacaactttt tgcttgcaca
     8281 aaatattcaa tcatatcacc tgactttcct atcatattgg caaaaaatat ttctgcttct
     8341 gtagtttctt ctaacctaat actttcccat gtttgaaaac catagtatcg ataaaacagt
     8401 ctacaatttt cataatctaa gccagcttta ctataatcaa ctatttgaat agtaacttct
     8461 aaagcatcat tctctgaaac gatttcttct aatcttttca cggaaatata gagcatattc
     8521 tcatcccaaa ttgctcttgt cctgcaatga ataacatctt catttcccca gccagttctc
     8581 acatatcctg tacgattttt tataatatct ggctcttttt caaaatcaaa ctcatatcct
     8641 ttaatttcat atccaggcaa agcgttctgc cattgtttta ttgcaatttt atcctgttca
     8701 attccataca ttggaacata gacacgacta tttaaaatca cagaattaac ataagcagta
     8761 agttcatcac tgtccttttg aaacggaaca atatctatac gaaagatttt gaatggacgc
     8821 ccatatctgg tttctacctt ttttacttct tctaaaattt tttcatatct ttcatacaaa
     8881 tagtgttctt ttggcgttct gctcacaagt aatgtttctt catcaattat ttttaaataa
     8941 cagtcaatat gttgtaaacc ataatcagca aaattagaaa atatataata ctcattcatt
     9001 ccagtatctc ttgccaccat agaaaaaaat tcttcgtcag aaattccttt cgcacgattt
     9061 tctgtctgta aaatgtgcat agacatcaat ttattgaaac catctgacat aagatttccg
     9121 cctgttagtg cataaggtaa tttcataaat ggcaaaccaa tctgtttcgc aatcaactct
     9181 tgtgattgat cttctttttg ttcataagag ttagtagtta ataggttatg ttcgcaatca
     9241 aacatgattt ctggtttatt tatggataca aatggtgttg acatcttata ttctgcacca
     9301 gccaaaaaga gttttccgtt ttcatcaaaa atagggtgca taccccaatc acgaacccat
     9361 ggtgtatcaa atccctgtgg agcaatgcag aaaatcaaat tttttaaatt tccaccccac
     9421 ttacttaaat aatattttgc aatttcaatt tcctctttat ttggtacaca catgtataca
     9481 gaataatttt gcgttaaatc tacaatatac ttatggggaa gaaaaggtgg ccatgcaagc
     9541 atgacacctt taatcggttc ccattcccca gctaaacgtc ttttcatttc taatacctca
     9601 ctttcttact ttttccacaa atgaaatgaa atttctctaa ttttaagatt tttcaacaat
     9661 gtttcaaatt catttttttg aaaggttttc tgattacata aaacttccat ttctagtccc
     9721 ttatactcca tgagatattc tgtttcataa aacccattct gaagataaaa ttgcttcctt
     9781 aaaattcttt gttgtaaatt aggagcactg tcctctatag tttccatatc aagaacaatt
     9841 tgacaattgg gatattgttt tttcataagt gacagtgctt ttccaccata tcctttagag
     9901 cgttgttctg gggaaattgc aagaaataat atatatccta tatgattttc aatcaccaaa
     9961 acataaaaac caataaattg ttgcttatca tataaagcag atacttctat ttttttactt
    10021 tgaactaaat taagcatttc ttgtatagac atacgttctt catctggaaa tgcctcgtta
    10081 ttaagttctt caaatttttc taaatcttta aaatatgaag ttactttttg tattgttaag
    10141 ctcatataga tacctccaaa ttgatttaat aatgatattt tttctgtttt cttattataa
    10201 agaaagccgt cataatagtt gccaatattt cagcaccaac aatagaaaac catatcccat
    10261 ttactcccca gaatataggg aatataagta ctgatacaac ttgaaatact aaaatacgca
    10321 taaaagcaat aagcgcagaa gttaaaccgt catttaatgc agtaaagaaa ctagaaccaa
    10381 aaacaccaaa accagaaaat aaaaaggaaa aagagtaaat cataaatgct cttaatgtca
    10441 tttccattaa attattatcg taacccacaa aaataaaagc aatcggtttt gataaaaatt
    10501 cgcctattat aaacataaat acagcaaaac aaataattac aatcaaactt ctttttctta
    10561 aattttttaa ttcgtcataa ttttttgctc cataatgata actgattata ggggcagtac
    10621 caacagaata cccaataaaa acagcttgaa atagtaacgc tacataaaga agaacaccat
    10681 aggatgcaac gccatcttcg ccagcatatt ttagtaattg agcattatac agcatactca
    10741 caacagatgt ggaaacatta gtcattagtt ctgaagaacc atttgcaaca gtctttttca
    10801 aaacatcaca atcaaattta ggtttcttaa atttaagcaa actgttgttt tgtcggctaa
    10861 aataaagtaa tggaatgata ccaccaacat attgaccgat tgcagttgcc agtgctgcac
    10921 ctaccaatcc ccatttaaaa acagtgataa ataatgcgtc taaaacaata tttgtaattc
    10981 cagccataat tgcaacatat aatccaagtt ttggcttttc tgctgttgca aataaacact
    11041 gaaattcgca ctgcaaaata taggcaggaa tagcgataat taccacacat ccataaagaa
    11101 tactatctgt caataattga ccatctgctc ccatagcaga agcaatccat ggcatagtta
    11161 ctatacctaa aacagctaga ataataccac aaccagcaga agtaacaatg agtaacgaaa
    11221 actgttcgtt tgcttcttct ttttttcctt cacccattgt tttagcaatt aaagctccac
    11281 cacctgttcc aaaaagaaag ccaacacacc ctaagattag caaaaatggc ataataaaat
    11341 tgacagcagt aaaagcagtt ttccctacaa aatttgaaac aaaatatcca tcaacaaccc
    11401 cataaataga acttattacc agcataataa ttgtaggaaa tgtaaatttt aataagcgtt
    11461 tataattaaa attatctgac aactgtatca ttgttcatac ctcctaaacc aatactctga
    11521 aaagttagca attgcataag aaagatgttt tatattccat tcagatgtat taactataat
    11581 atgataggaa gtttgatttc cccataagga gctcgtaaaa aattctcttg atcttgctcg
    11641 atttttatca atctctctta tctttctttc taattctttt tttgaaatat gttcattgtt
    11701 taactttttt tcaatacagc gttgtatttt aaagttcata tttgcacaaa caaaaacgtt
    11761 aaatggctgg tacttcttta ataatatatc tgcatttcta ccaacaataa cacaatcctt
    11821 acctgtactg gcaatctgat tgataacatt tgattgttct attaacaaat ctgtcttcat
    11881 ggattgcata gacaaaatat tagtaaaaga agtacacatt gttaaaggaa cgtttctcca
    11941 accatgattt tctaatatgt aggaaacata gttttcgtca atttttttct ttctagctat
    12001 agcagtgata atttctttat cgtagtagtc aaaatttaaa atatcagcaa gttgttttcc
    12061 aagttctcgt ccaccacttc caaactctcg gctaattgta ataattttca taatatcttt
    12121 tcctcacatt tcattgaata taaaagcttt ctatgttatc ctaaagtatc tggtaaccct
    12181 aaagtcaata ggagatttaa aaatgattaa aaaattatat aagacaagtg aatttgcaga
    12241 attgtgtggg gtaaaaaaac atacacttta ccattatgat gaaattgaat tattaaaacc
    12301 gagtattacc catgaaaatg gctatcggta ttatacaatt gaccaatttg aaaaatttaa
    12361 tattatttct gtgctaaaaa aagcaggtac acctttagaa gaaataaaac aatatctcaa
    12421 tgatttagat acgaatacct ttcttaatgt acttcacaga aagctaatgg atcttcaaga
    12481 agaagtgaag aagattgaaa aaatgtgttc tatgttgaga aatgtcattt caacaatgga
    12541 aatacatctc aatgtaaaca cagaaaaaat cgaaattgta aattgtgagg aagaatatct
    12601 gattgcaact aaaattcctg aagctgtact aacagatgaa aaaacaatgt tacaaggtat
    12661 tggaaatcac ttacaatatt gtttattaca tgattataat tttcatgtag gagaaattgt
    12721 tcttgaaaaa aatataaaac agggaatttt tagagagagc tattattata gccttattaa
    12781 aaataaagta gatgatgagc gcctttttat taaaccaaag ggaaattatg cagttatgta
    12841 tcaccagggg agttatgaca gtttatataa aacttatcaa aatatgaaaa atcagttaga
    12901 agaacaaggg tatcatattg ttggaaatat ttatgaagaa gatttaattg attatttatc
    12961 agaacataac gccgaaaact acattcaaaa aatttctatg caggttattt gaaaatataa
    13021 aaagcgtgta tatctcaaat cacatgagaa atacacgcct tttttaatat ctcgtattgt
    13081 ctataataat ttgcttaaaa tgtccttttt tatcttctaa agttggtatc ttatttccaa
    13141 atttttgtag aaattcaggt tcattgctat tatttgtttc ctcaatgcaa actgcatttc
    13201 ctcatgaatt tcagatacag aaatagcctt ttattttcgt taaccattta atcagttttt
    13261 tactatcttt ttataaaatc ttaacatgta ggctttatgc ctccatttct tttgtagttg
    13321 tcttaaaagt agtgcaaatt tttttcttaa atatatatta tataaattat tcatttatat
    13381 agagtattat attatgctct gtaaatagtt acaatattta aaagaatgaa aggagaacaa
    13441 aaactatgga aattgattat aaagcaatcg gacagcgaat taaaattgca agaattaaaa
    13501 aaggtataac acaagaatcc gttgcaaaca ttatcaacat gacaccatcc catatgagca
    13561 acgtggaaac aggaaaaacg aaagtcagcc tgccaacctt gattgcgatt gccaatgcac
    13621 tttctgtatc agtcgatact ctcttatgtg ataatgtact ttcttccaaa gtaatttttg
    13681 aaaaggaagc caaagatatt ttctcagatt gtgatgaata tgaaatccgt tctttagttg
    13741 aactgctaaa atcagcaaaa actgtaatcc gtaaagacag agaaatccgc aaacaatttg
    13801 aaaaataaaa ccacagcttc aaggtataaa ttgatatgtg ttgttagcta aggtcttttc
    13861 tttttttata taatcatttt tatataatca aataacctat ccaataattt tccaattact
    13921 gtccttatga aatatcaatt tatactatga tacctgtgtc gcttttgtct gattatcaag
    13981 gaattttaca gctacctttt cattatcaac aacaacagta aaaatagggt ttaccaattc
    14041 agaaagtaaa tattcttgaa atatactatt ttcctttcat aaaaatgata aaattttatt
    14101 ttacatccca cgtaatgaag tgttcaataa ataagttaaa ataatttatt tacctcctta
    14161 atcatatttt ttatgaatac ctaataacac agtatcaata gaatcgacta aaccttttat
    14221 agcttcttct ttactcaaaa gtgatttccc aagttgcaaa gtcgaataac catgaagtac
    14281 actcattatt aagtttaata cttcacctgt attctttgta tttagattac aagaaagaat
    14341 aagtttttct aataaggatt tatattcatc aaagatttct aatgtttcat tatttccatg
    14401 ccaagtagcc aactgaattg tttcataaac accaggatta gaaattataa aattaaagta
    14461 tgctatactt atacacttta tagctgtatc tccaaaattt ccaatagccg cctttatcat
    14521 ttgactattc atctctctca ttcctttatg agctacttca agtaataaat catctagact
    14581 ttctatatga ttgtacaaag atggtgtacg tattcctagc ttttctgcaa taacttttaa
    14641 agaaactttg ttaagcccat ctttattaac tatatctgat gtagcttgta taactatttc
    14701 tctactaaca cgcatgattt tccctctttc atttaaaatt aattacttta ttagtttagc
    14761 taatctaatt agtttcgtca attattgtaa tttataaaat tctttatact attataaaat
    14821 cttgacagtt taattataat ttaatataat actaattgaa ttagttttaa tactaatata
    14881 attagtatta tattaaattc tatattacaa cattatttta tagtttgatt aattcaacaa
    14941 tcactaatat atcaaactga aaggagattt ttatggaaaa aaaatatttc gaaattcatg
    15001 gagttccttc aatcctttgg ggaaatcctt caagtaaatt atatatttac atacatggac
    15061 aatgtggcaa taaagaggag gctgaagctt ttgctaatat tgcagttcca catgagtgtc
    15121 aagttttaag tgttgatttg cctgaacatg gagaacgtaa aagagaaaaa aattcattta
    15181 acccatggca tgttgttcca gaattaatat ctattatgga atatgcaaag tgccactgga
    15241 atcaaatttc gttatatgca aatagcattg gagcatggtt tagtatgtta agctttgaaa
    15301 aagagaactt aagagagtgt atttttgtat ctccaattct agatatgcaa cagctcattt
    15361 caaatatgat gctttgggca aatgtatcag aagaacagct taaacatgaa ctaattatcc
    15421 caacttcatt tgggcatact ctttcatggg aatatctaca atatgtaaaa caacatccta
    15481 tcactcaatg gaatgtacca gcaaaaatat tatatggtaa acatgatgaa cttactgaaa
    15541 tcggtgttgt agagaaattt gtagaaaagt ttaattgcaa ccttactatt atagaaaatg
    15601 gcaaacattg gtttaacaca ccacaagaat taaattcatt atatgaatgg ttaaatactt
    15661 tagttaaata aaaaataatt atattatt
//
LOCUS       DAENCV010000087        15620 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAENCV010000087 DAENCV010000000
VERSION     DAENCV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.088"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(101..835)
                     /locus_tag="KQD82_002709"
     CDS             complement(101..835)
                     /locus_tag="KQD82_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG4381169.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            960..1676
                     /locus_tag="KQD82_002710"
     CDS             960..1676
                     /locus_tag="KQD82_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG4381170.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDNDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1810..2502
                     /locus_tag="KQD82_002711"
     CDS             1810..2502
                     /locus_tag="KQD82_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381171.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2492..3673
                     /locus_tag="KQD82_002712"
     CDS             2492..3673
                     /locus_tag="KQD82_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG4381172.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTMFEVQLPIIQS
                     A"
     gene            3955..4410
                     /locus_tag="KQD82_002713"
     CDS             3955..4410
                     /locus_tag="KQD82_002713"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG4381173.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4400..5125
                     /locus_tag="KQD82_002714"
     CDS             4400..5125
                     /locus_tag="KQD82_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG4381174.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5097..7478
                     /locus_tag="KQD82_002715"
     CDS             5097..7478
                     /locus_tag="KQD82_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG4381175.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKNRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7683..8912)
                     /locus_tag="KQD82_002716"
     CDS             complement(7683..8912)
                     /locus_tag="KQD82_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG4381176.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9513..10427
                     /locus_tag="KQD82_002717"
     CDS             9513..10427
                     /locus_tag="KQD82_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381177.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10451..11086
                     /locus_tag="KQD82_002718"
     CDS             10451..11086
                     /locus_tag="KQD82_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG4381178.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11462..12178
                     /gene="pyrF"
                     /locus_tag="KQD82_002719"
     CDS             11462..12178
                     /gene="pyrF"
                     /locus_tag="KQD82_002719"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG4381179.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12204..13247
                     /gene="carA"
                     /locus_tag="KQD82_002720"
     CDS             12204..13247
                     /gene="carA"
                     /locus_tag="KQD82_002720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG4381180.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGIKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13300..>15620
                     /gene="carB"
                     /locus_tag="KQD82_002721"
     CDS             13300..>15620
                     /gene="carB"
                     /locus_tag="KQD82_002721"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG4381181.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         6296 a         1725 c         2807 g         4792 t
ORIGIN      
        1 ataataacat ataataaata aaaaagtaag attgtgtaga ttatataaag attatttaca
       61 attaatacaa tcttgctttt ttatttaaaa attttaatta ttatttaaaa ttttttatct
      121 tctctttatc ccatataaac aatttctcag aattatttaa agcaccaaat acatttcttt
      181 taaaatcagg catctcttta tttaaattag caataagatt ttttatgtac tctctcctag
      241 tatgaccatt tgcagggcag ggatttgtga taataggata gttagaatct tttgaaattt
      301 tcttagtcat atactcttct atgtaaacca taggtcttat aactgttaaa tcctgtctgt
      361 ctaggtatgt ctttggggaa aaacaattta ctctaccctc ataaaacata gacattaaaa
      421 atgtctcaat tgcatcatct ttatgatgac caagagctac tttattacat ccaagttttt
      481 tagcattatc gttcaaagca cctcttctta aattagcaca taatgcacaa ggattctttt
      541 cttgtcttat atcaaataca atttctttta tgtcagtttg aaattcataa aaaggaacat
      601 ttatttcttt acacaattta tctagtggag aattatcaac acctccagta tttaatgtta
      661 tggctatcaa ttcaaagttt tgtggagaaa atctttgata acttttaagt atatgaagta
      721 aagttaaact atcttttcca ccagaaagtc caaccgcaat tttatcattt tcttgtatca
      781 tatcaaaatc ttgtatagcc ttacgagctt tacttagaag tttttgtata gacattttat
      841 caccatcctt taaaaatatt aaatttaaga tttatttaag atttacttat aaaatactta
      901 agtcttatac tctaaaatta acattatagc aaacataaca aaaaggagaa gatttaatta
      961 tgaaaaaatt taaattaata ggagccatta ttttagtagt atttataagt gtatttatat
     1021 ctaaagatag attacttaag aaagataacg atccaatagt agctgctcaa catgattcag
     1081 aacaagatgt tgaggcaaat tctcaaaata aaaatcactc aaatagtata tcaaatatat
     1141 tattggtaaa caagacaaat ggaattagca aaaactatac tcctgaaaat attactaagg
     1201 taaacatacc ttttgttgaa gaagcaacag aggaagaaaa acaaatggca ggagaacctg
     1261 caaaggcagt tgaagattta gttaagcaag ctaattcaga aggaatacag tttttagggt
     1321 cttcagcata cagatcttat gatactcaac tggatactta tacaagaagg gtaaaatctc
     1381 agggacgaga aaaggcagat gcatatgtag caaagcctgg ttacagtgag caccaaactg
     1441 gactttgtat tgatttaaca aatccagaaa gatggtttgt tggaagtaca aaagaagcta
     1501 aatggttggc agaaaatgct cacaagtttg gatttataat aagatatcct gaaggtaaag
     1561 aagatataac aggtacagca tatgaaccat ggcatattag atatgttggt aaggatgctg
     1621 ccgaggaaat ttattcaaaa ggattaactt tagaagaata tttacaaaat aaataatata
     1681 aatgtgtaaa taatttgtat tatattggaa atttagaaat taataaattt atagattaca
     1741 ctaactaaaa tatagtataa ttttatatga ataaagctat atacaaagat taagttagga
     1801 tgtgtaaaaa tgaataatac agttcttgtt gtggatgatg aaaaggaaat aagagacctt
     1861 atagagatat atcttttaaa tagtggatat aatgtaatta aagctgaaga tggaaaagag
     1921 gctctagata ttttaaaaaa ggaaaatatt catttaatga tattggatat aatgatgcct
     1981 aaaatggatg ggatagaagt ttgtagaaaa gttagagaaa gtttgaatat accaatactt
     2041 atgttaagtg caaaatcaga agacatggat aaaatacaag ggataatgac tggtgcagat
     2101 gactatttga caaaaccatt taacccactt gaacttactg tgagagtgaa gtcgctgatt
     2161 agaagagcat attattttag tggtgctaat aatgaagata aagatgtcat aaaggttgga
     2221 gtagtaatta tagataagga aaaacatagt gtaacagtgg ctggaaatga aataattttg
     2281 acatcaagag aatttgatat attatattta ctagcaaata ataaaggcag agtatatagt
     2341 acagaagaaa tatttgaaaa ggtatggaaa gagaaatatt accaatctaa taatactgta
     2401 atggttcata tgagcagaat aagagataaa attgaaaaat atacagatgg agaaaaaata
     2461 attcatactg tatggggagt aggatataaa attgaaaaat aaaagtttat taaatcatat
     2521 gtttcgaccc atattagatc ttataataag ctgtgcttta acttcaatgc ttattttaac
     2581 aagtatgtat ataataaaat cattatatgt caatagtgat atgtctgtag ttaatcaact
     2641 tgtatatcaa gcaagagatt tcagaagtct tatagggtat gatttatttt atccattttt
     2701 atttacaatc acatcattag caatttattc cattatatcg tacaagagaa ataagcaaat
     2761 tgtagatttt ataagaaaga ctaatagaaa aaatcaaaaa ttagaaatca ttaaaagtgc
     2821 tgtaaattta atggatgaag gtaacttaga aaaaagtata gatattgagt atgatttaga
     2881 tatattaaag gataaagaaa aggatgatat tgatgaacta gcacataaaa tcaataatat
     2941 agtccatcaa cttagaaata taactattga agagagacaa gctcagcaga caaaaacaga
     3001 tttaataaca aatgtgtcac atgacttaag aacacctttg acatccataa tgggttattt
     3061 aggagtgata gaagaaggta agtataaaga tgaagttcag atgatgtatt atgttgatat
     3121 tgcatatgaa aaatcgaaaa atttaaatgt tcttataaat gatttgtttg aattgactaa
     3181 aatgcaaaat aatacaataa aattaaacaa aatagaaata aatttagtag agttattaag
     3241 tcaagtagta tctcaatttg aaatttattt taaacaagaa tttatggtta gtagaattaa
     3301 ttttaatgaa gaaaaattaa ttgtaaaagc tgatccaaat aaattggtaa gagcatttga
     3361 aaatcttata actaatgctg tgaaatatgg aaaagatggg cattatgtag atatagtcac
     3421 agagaaaaag gaagatatgg cggttgtaaa agtgataaat tatggggaac caattccagt
     3481 gttagattta ccaaatatat ttgatagatt ttatagagtt gaaaaatcga gaaatagaaa
     3541 tgttggaggt tctggattgg gtcttgcgat tacaaaaaat attgtaaatt tacatgatgg
     3601 agaaattact gtttcaagcg ataaatatca aactatgttt gaagtacaac tgccaataat
     3661 acaatcagct tagttataaa ataaaagtac gattatgtga tataaatatt ataacaataa
     3721 agtgtttaca aaaataaaaa agtaatatat aatttaagtc agtttaataa gatacttatc
     3781 aagagttagg ggagagagtt agctcagtga tcctatacca accttttatc aggtggtcct
     3841 gctaacaacg atgagatgac ttgaaataat atactcttct tatcaataat agataggaag
     3901 agtttttttt agaattaatg taacaaattt taaagtataa tggaggatta aataatggaa
     3961 aaagtagaaa gttttaagct agaccataca aaagtaaaag ctccttttgt aagaaagtgt
     4021 agtgtacttg atggagtaaa gggagataaa gtaactaaat ttgacttgag atttttacag
     4081 ccaaatgtag aaagttttgg aactgctgct atgcacggtt tagagcatct tttagctact
     4141 tatttaagag atacattaga tggtgtaata gacttatctc caatgggatg tagaacagga
     4201 ttttatttaa tactatgggg agatgtagat gccaaaacag ttaagattgg tttagaagaa
     4261 gcattaaaaa aagttcttga atcagataaa atgccagcag ctacagctat agaatgtgga
     4321 aactacagag acttatcttt atttggggca aaggaatatg caaaagatgt tttagataaa
     4381 ggattttctt taaatatata tggagaataa tttaattgga agagaagata taagcataaa
     4441 tcaaagtgaa gttttaagat atttacaata cagaggtcaa gagatagata aagaccttaa
     4501 atatactatt gatgaatgtg tagctataac taaaaataaa ataaatccta gatatttatc
     4561 taggatttat cctattaaaa taaataaaga taatgttgta gaattagagg gaacaaattt
     4621 aacattagag agtaaagatg tatatgaact tttaaaagaa tgtaatgagt gtgtaattat
     4681 ggcagctact atagggatag atatagaaaa agaaataaga cgacactctt attcaaattt
     4741 aacaaaagga ctaataattg attcatgtgg aacaacagca atagaagaag tgtgtgacat
     4801 tgttcaaaat aatttagaaa aggttttttt aaagcaagat aaatttataa ctatgcgata
     4861 tagcccagga tatggagatt taccaataga aaaaaatata gatatattaa acgttttgaa
     4921 tgctcaaaaa caaataggtc taacaattac ttctaatgga attatgatac cgagaaagtc
     4981 agtagtggct attataggta tttctaatac aagaataaat aaatctaaga aagaaaagtc
     5041 gtgtgaaaat tgtaaaaact atagaaactg tacatataga aaaggagcga actcttgtgg
     5101 aaataagaca atatctgaaa aataatatat taatatttga tggtgcaatg ggaacaatgc
     5161 ttcaacaaaa agggttaaag ttaggtgaaa atccagaagt atttggtctt caaaatccag
     5221 ataaacttat agaaatacat acagcatact tagaggcagg ttcgaatgta attcttacaa
     5281 atacttttgg atgtaatgaa ctaaaattag atagtaagta tactgtagaa gaagtgattg
     5341 acaatgctgt tttggtggct agaaaagcta tagaaaatgt agataatact aaaccaagat
     5401 atgtagcttt agatattggt cctataggag aaatgctaga gcctatgggt actttaagtt
     5461 ttgataaagc atatgaaata tttaaaagac aagtactaca aggcgttaaa tctggagttg
     5521 atgtaatagt aatagagact atgatggatt tatatgaagc taaagtagct gtattagcag
     5581 caaaagaaaa ctctgattta cctattttct gtactatgac atttgatgaa ggtggaagaa
     5641 gttttactgg atgtatgcca gaatgtatgg tggctactat agaaggtcta ggtgtagatg
     5701 caataggagt taattgttca ttaggaccta aacaactttt accaatagtt gaaaaaatag
     5761 catcaagagc tacagtccct gttatggttc aagcaaatgc aggtcttcca aatatagttg
     5821 atggagaggc tatatatgat gttgatgcaa aagaattttt tgaaggggtt aaaaagttcg
     5881 ttgaagttgg tgctactata ataggtggat gttgtggtac gaatccaagt tttataaaag
     5941 aaatatctga gaatataaat tctgttacaa agggatgtat cgagaaaatt gataaatgtg
     6001 tagtctgttc accatcaaaa tttgtggaag tacaatcgcc tacagttgtt ggagaaagat
     6061 tgaatcccac aggtagaaaa tctctacaag aagcattaaa aaatgaaaat gttgattatg
     6121 ctataaattt aggtttagaa caagtaaatg ctggagcaca aatacttgga gtaaatgttg
     6181 gtcttccaga gattgatgaa aaaaaactaa tgccaaaatt aataagagag attcaagcag
     6241 tagtagatac acctttacaa gttgattctt ccaatgtaga ggccttagag caaggactta
     6301 gatattataa tggtagaaca atagtaaact ctgtaaatgg caaagaggag tctttagaaa
     6361 gtatattacc aatagttaaa aagtatggtt catgtgtggt tggacttaca cttgatgaaa
     6421 aggggatacc taaaaaggct gaagaaagat ttgaaatagc aaaaaggata gttaatagag
     6481 cagtttctta tgggataaaa ccaaaagata tatttataga ctgtttatca ttaactgttt
     6541 cagcacaaca agaagaagca atagaaacta taaaagctat aacaatggta aagacactag
     6601 gagtaaaaac tatattaggt gtttcaaata tttcatttgg tcttccaaat aggaaagcac
     6661 ttaatgcttc ttttctaaca ttagctttag gggcagggct tgatttagca attataaatc
     6721 caaatgaata tagtatgatg gaagctatca attcttttaa aatattaaat aacacagata
     6781 aaggatgtat aaactatata aatcaatata gcaatataaa taattcaaaa aagtcggatt
     6841 catctacaaa gattgataaa gatttgccct tggatatttt agttgaaaga ggattaaaag
     6901 atgaagcaaa aaatgtaaca ctgaatcttt taaaagagaa ggatgaaaat tatatattag
     6961 atgagatttt aattcctgct cttgataaag taggaaaaag atatgatagt ggagatatat
     7021 ttttaccaca attgattcaa tcagcagaaa cggtaaaagt ttcattaaat acaataaagg
     7081 aaactttatt aagtaaaagt agcaataatg tatccaaagg aaaaataata gttgctacag
     7141 taaaaggtga tatccatgat attgggaaaa atatagtaaa aataatgcta gaaaattatg
     7201 gatatgaggt tatagattta ggtaaggatg tccctattga agaagtagtg gaagttgcta
     7261 aaaatagaaa tataaaatta gtaggattaa gtgcacttat gactacgact gttcaaagta
     7321 tgaaagatac aattcaggca ctgagagata atgagataaa tgcaaaggta tttgtaggag
     7381 gtgcagtttt gacagaagaa tacgctgaag aaatgggtgc tgattattat tcaaaggatg
     7441 caaagtctgc tgttgaaata gcaaagttaa atttttagca tataaattaa aataaaggtt
     7501 ttactaaata ggatatttta tatataatat aaatataagt tttagatact tatgtttaaa
     7561 acaataggaa ttaatctata ataatgagat taattaatat aaaaaatatt tatcatcaac
     7621 attaaaaagg gctgggaagt ctggtataac ttccatcagc cctttttagt taaaatcata
     7681 tgttaaaaag cccaattacc atttttaaaa atctgtattt gctttccatc ataagtctca
     7741 ccaataatat ccatatcagc agaaccaatc ataaaatcta catgtgttaa actatcattt
     7801 acaccatgtt catccaattc ttcatctttt aaattgtttc caccttccag acaagtctta
     7861 taagcagaac caattgcaaa gtggcaagaa gcattttcat catatagagt attataaaaa
     7921 attatatccg tattggatat tggcgagttg tatggaacaa gagcaacttc tcctaaataa
     7981 tgagagcctt catccgtttc aataagtttt tctagtgtat ctagtccctt ttcagcagag
     8041 aaatctatta ttttaccatc tttaaatgtt aagaagaaat tgtcaattat gtttccacca
     8101 taaacaagag gttttgtact ataaacagtt ccatttactt taaacttatg tggcattcca
     8161 aaaatctctt cagttggaat attcggatta aaacttattc cattagggtc tttggatgct
     8221 ccacttagcc aaacatgacc ctttggcaag tctagagtca aatcagtttt tgaggattta
     8281 tattttaaag ttttaaagtt atttttattt agaaagtcca ttttagattt aagattttca
     8341 ttatgttctt cccatgcttt tatagggtcc tgaccatcta ctcttgaaca tttaaatata
     8401 gcatcccaca gtttttctat tgctttatct tctgataaat ttggaaacac tttttttgcc
     8461 caatcttttg ttggcacaga gactatactc catttacatt tatcagttag agtgtaagaa
     8521 cgccattctt ttaaagcttt tccagatgcc ttctgaaaat ttgcaactct ttcagggtca
     8581 acatctttta ataaatctgg attttgggca tagactgata aaaatgcccc accttctttt
     8641 gctatatcta catattgttc tgctgtccat ttggggaact catgaaatgc ttcatctgga
     8701 gcatataagt atttaataag agtactttct tcatcagacc actctatata aacattttta
     8761 gcaccacatt tatatgcttc tttaagtgca tttcttgcaa aatcagcaca ttctattggt
     8821 gactttataa gtagaatttg acctggctgt atgttaactc caacttttat tgctaattca
     8881 gcgtattttt ctaaatttct atttaaattc atatttatcc tcctatattt ttaatattga
     8941 ataacttata aagtattata acattactta tgtttttaat attgaacaac ttataaaata
     9001 ttataacttt acttagattt tgggtcatat tcaaaatatt tatactttat agattgagaa
     9061 ataaaaaaaa tattttaatt aaaacaaatt atgaggataa caaacttatt taagcagtat
     9121 gttttttgtt gataataata ttaaagtaga aataaaaata cttttttagt gaaaagctat
     9181 acaagaatac cgaaaaaaac ttataatata ataatgagat agatatttat aaatgaaaaa
     9241 aaatttttct ggcaaagctt aattaaaaag gtaatagcta gtgttgttat ttaaattagc
     9301 atagaaaatc aaataaaaaa attatgctaa tgtatagaca ttgataaggt agaatttaag
     9361 ccttttgaat gctatattaa actagagtag atattcttat gaatttcaaa atagggcagg
     9421 aggaaaaagg aaataaaact ataaaaaata taaagtattt atataaattt atagagttta
     9481 tacgtcagaa gatttcagtt aggagggcat aaatggagct ttatgaagcc attttttata
     9541 gaaaatctgt aagaaatttt tccagtaaga agataaaaaa gccattgatg gaagaaataa
     9601 aaagaagttg taaaaattta gaatacttaa atgaagattt aaatattaaa gcacatgttg
     9661 ttgatagagg tcatataata cattttttga tgggtaagga atgtaaagta aaggcacctc
     9721 attatatagt agttacatca gacaaaggtg aggactattt acagaatata ggatttgcta
     9781 cagaaggtgt agttttgcgc cttacaacat tgggaatagc tacttgctgg ttggaaggaa
     9841 acttaaagag agaagatatc ttggaatttg tagatattgg gaataagaat tttgaagaag
     9901 atgaggaaga tgatttagat tatctagctg gaataaaaac agaagaagat gatgaaaaca
     9961 aaatagaaaa tccttatata ataatagcat ttggatatcc agcaaaaaat gaagagttgt
    10021 ttaggacgag atatgctaga cctgatagaa agccagtaaa agatatgata aataagataa
    10081 ataggaatag aaagtggaaa gatatcttag aattgacaag aagagctcca tctgtaaaaa
    10141 attctcaacc gtggtatttc cataaggatg aacgaggtct tcatttattt gaaaagagac
    10201 ctaagaaaca ctgtgaagat atgaataagg ttagcttagg tgtagcctta agacattttg
    10261 atatagcttg tataaaaaat aaaatagatg tttcatatga aaaacttcct ataaggaata
    10321 aaataggaaa atcatatttt ataactgtag tcgagcatgt gaaaccagaa gaagaaaccc
    10381 aagaagaaaa tgtaactcta gaaaaagaag aatcgcaaga tgaataaaat tttggaaggg
    10441 gatattggat atgtataagt gtggaaaatg taatcttaga ggctgttcta atggaaatat
    10501 ggataaatta atgaagatat gtccaactag agaaattgaa ctacaacaaa aagcaaagca
    10561 actttatgaa gaagaagaga atagaaaaat agcttataat gctggattag ttgaagcaga
    10621 ggggtattgt caatatacta gactaaaaga gattatagag tttgctaaaa agtgtggtta
    10681 taaaaagatt ggcatagcat tttgtattgg actaaaaaga gaaatgcaaa cagtgcaaaa
    10741 aatatttgaa catcatgggt ttgaagtatt ttctgttata tgtaaaaatg gggcaatgcc
    10801 taagagtaca attggactag aagcatctca aacaatatca aagtgttcag atgaggtaat
    10861 gtgcaatcca ataggacaag ctttattttt aaatgaagct aaggttgacc ttaatgtaat
    10921 tcttggatta tgtgttggtc acgatacttt agccatgaaa tatatggaag ctcctataac
    10981 aactttagtt gtcaaagata gagtaactgg gcataatcca gttgctgcaa tatataatgc
    11041 agaatcatat tttgagaaaa aattgtttga taaagatgaa gaataattca aagacttatt
    11101 gacataaaaa gcaattagtg gtttaattat aaaaaagata aaactttaaa ttatcctata
    11161 gatacataaa gttatataaa taaagaaaaa tattacagct aaattcctta aagtagtcca
    11221 gagagactaa agggttatag ataatgttag tatataacct ctagtaaatc ttctagaggt
    11281 ttttgatgag tataaaataa aaatttaaat ataataaagt cttttaaact agtccagaga
    11341 gactgggaaa gatatctcat agctaatgaa tagagttatg tatagataaa atccttatgc
    11401 ctcttttggt gtgaggattt ttttatgatt atttaggctt tattaaaaag gagatgtaaa
    11461 aatgataaat ggtaaagaaa aattgatagt tgcaattgat actgacgaat ttgataaagc
    11521 taaagaacta atagacagat tagaagatag tgtagacata tttaaggtag ggctagagca
    11581 atacgttgcc acaaaaggta aaacaataga ttatcttaaa gaaaaaggga aaaaaatatt
    11641 tttagacctt aaattccatg atataccaaa tactatgaaa agtgcagtga gagctgcagt
    11701 gagagataat gtttggttaa tgacaataca tgtatcagat atggaaggaa tgagacaatg
    11761 tgcactaatt gctagggaag aagcagaaag attaaatata gaaaagccat tagtagttgg
    11821 agtaacagtt ttaacttcat taagcaacca agatttacag gacataggat gtaatatgac
    11881 aacagaagag ttagctataa agagagctaa actggcaaaa gaatcaggaa tagatggggt
    11941 tgtttgttca gcgcaagaag tagataaaat agtagaagca tgtggaactg attttataac
    12001 agtttgccca ggaataagac ctaaatcagc agaggttgga gaccaaaaaa gagttgtaac
    12061 tccatcagat gcaataaaaa aaggtgctca ttatttagtt gttggaagac ctataacaaa
    12121 agcagaaaat ccaagagaat cagctattaa tattgtaaga gaaatagaaa atgcttagat
    12181 aaaatatttg ggaggtagct tatatgaagg ggaaattaat tttagaagat ggaacagtat
    12241 ttgaaggaaa agcatttgga tatctaaagg aagctattgg agaagttgta tttaatactt
    12301 caatggctgg gtatggagaa ctattgacag atgcaactta ttatggacaa atagttacta
    12361 tgacttatcc acttatagga aactatggaa taaatttaga agatgttgaa tcaaataagg
    12421 tgcaagtaaa aggacttatt gtaagagaaa aaagtgataa cccaaataat tttagatgtg
    12481 aaatggatat agacacatat ctaaagcaaa ataaggtaat aggacttgaa ggaatagata
    12541 caagagcatt aactaaaatt attagaaaca atggaactaa gagaggaatt ataactttag
    12601 aaaatgtatc attacaagat gttcagtcta aattagaaga gcattgtaac aaagattcag
    12661 taaaaaatgt tacaagaaaa gaaattgaaa atataaaagg tactggagct aaagttgtag
    12721 ttatagactt tggtattaaa aatagtgtac tagagtctct taaagcttgt aactgtgata
    12781 taactatagt accagcaatg actacagtag aagaaatttt aaatataaat cctgacttaa
    12841 tcttattatc taatggccca ggaaatccag aggatataaa tgatatagtt gaaaatataa
    12901 aaaaattaat aggcaaaaag ccattgacag gaataggttt aggacatcaa attttagcat
    12961 tggcattagg aggaaagact tcaaagcttg tatttggaca tagaggaggt aatcacccag
    13021 ttaaatgctt agaaactgga aaggtattta taacttctca aaatcatgga tataatgttt
    13081 cagaaatgcc agaaaataca gaagtaactc acataaattt aaatgacaat actatagaag
    13141 gaatgagaca taaggaatta ccaatatata gtgttcaata cattccagaa tacacaacaa
    13201 aagaagattt agattgtata tttaataaat tcttagcatt aatatagatt tagtattatt
    13261 tacagatagt aaaaaccatt tagataagga gaaaaattta tgcctaaatt agatagtata
    13321 aagaaaactt tagtattagg gtcaggacca ataataatag gtcaagctgc agagtttgac
    13381 tattcaggaa cacaagcttg ccagtctttg aaagaggaag gaattgaagt tgtattaata
    13441 aatagtaatc cagccactat aatgactgat aaagaagtgg cagataaaat atatatagaa
    13501 ccattaacaa tagaatttat agaaaaaata atagaaaaag aaagaccaga cagtttactt
    13561 gcaggtatgg gtggacaaac agggctaaat ctagcagttg aactttatga aaaaggcata
    13621 ctagataaat atggagtaaa gataataggt acttcagtag aatctatcaa aaaaggtgaa
    13681 gatagagata tatttagaga agtaatgaaa gaaataaatc agcctgtaat agttagtgat
    13741 atagttacag atttacaagc aggtcttgat tatgcactta caattggtta tccagtagtt
    13801 gtaagaccag catacacact aggtggaact ggtgggggta tagctgataa tgaggaagaa
    13861 ttaagagaaa tactttcaca tggtctacaa ttaagtcctg ttggacaagt attaattgaa
    13921 aagagtataa aaggttggaa agaaatagaa tatgaggtaa taagagattc aaagggaaat
    13981 tgtatagttg tatgtaacat ggaaaatata gacccagtcg gagttcatac aggagacagt
    14041 atagttgttg ctccaactca aactttaagt aacaaagaat gtgcaatgct taaaaaagca
    14101 tctttagata tattaaatgc agtagaagtt caaggtggtt gtaacgttca atttgcactt
    14161 aatccacata gttttgaata tgcagttata gagataaatc caagagttag tagatcatca
    14221 gctttagcat caaaagctac aggatatcca atagctaaag tttcgtcaaa aatagcttta
    14281 ggttatactt tagatgagat agaaaatgca gtaactaaaa aaacttatgc ttgttttgaa
    14341 ccaactatag attatgtggt ggcaaaaata ccaaaatggc cttttgataa atttaaaaaa
    14401 gcaaatagaa aattaggaac taagatgatg gcaactggtg aaattatgag tataggaagt
    14461 aattttgagg ctgctattct taaagggata agatcacttg aaacaggaaa atattcatta
    14521 gttcacacac catctgagga gagaagcata gaggaattaa agaaaagagt tgtagtacct
    14581 gatgatgaga gactatttga tttagctgaa atgataagaa gaggctataa agtagaaatg
    14641 atagaacaaa ttacaggtat agataaatgg tttataaata aatttaaatg gatagtagag
    14701 caagaagaaa aattaaaagt tatgaaaatt gaagatttaa ctaaagacta tcttcttgaa
    14761 ttaaagaaaa agggattctc tgataaaggt atatctgacc taatgaagat aagtccagaa
    14821 aaattatatg aattgagaag tctatacaat ataaaaccag catataaaat ggttgatact
    14881 tgtgcaggtg agatagatgc tatatctcct tactattatt caacttatga gcaatatgat
    14941 gaggtagttg ttagtgataa gaaaaaggtg atagtattag gttctggtcc aataagaata
    15001 ggtcaaggta tagaatttga ctattgttca gttcactgtg taaaatcttt aagaaaaatg
    15061 gacatagaaa ctataatcat aaacaataac cctgaaactg taagtacaga ctttgataca
    15121 tcagataagc tgtattttga accattaaca gaagaagaag ttttaagtat aatagaaaaa
    15181 gaaaagccag aaggtgtaat actacagttt ggtggacaaa cagctataaa attggctaag
    15241 tttttacatg aaaaaaatat acctatactg ggaacagatt ttgatgatat agatgcagca
    15301 gaagatagag aaaaatttga tgatttacta gagagactag atataaatag accaaaagga
    15361 aagggtgtat ggtcacttaa cgaaggtatt gaagttgcta atgagttagg ttatccagta
    15421 ttagttagac catcttatgt acttggtggt caaggtatgg aaataactta taacgaagaa
    15481 aaattatctc aatacctaca agatgcattt gatagagacc ataaaaatcc agtacttata
    15541 gataaatatc taactggtag agaaatagaa gtagatgcta tatgtgataa ggaagatata
    15601 ttgatacctg gtataatgga
//
LOCUS       DAENCV010000088        15571 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAENCV010000088 DAENCV010000000
VERSION     DAENCV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.089"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(26..886)
                     /locus_tag="KQD82_002722"
     CDS             complement(26..886)
                     /locus_tag="KQD82_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG4381182.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1203..2531)
                     /locus_tag="KQD82_002723"
     CDS             complement(1203..2531)
                     /locus_tag="KQD82_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG4381183.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2679..3008)
                     /locus_tag="KQD82_002724"
     CDS             complement(2679..3008)
                     /locus_tag="KQD82_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG4381184.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKYHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3032..4321)
                     /locus_tag="KQD82_002725"
     CDS             complement(3032..4321)
                     /locus_tag="KQD82_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG4381185.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4337..4663)
                     /locus_tag="KQD82_002726"
     CDS             complement(4337..4663)
                     /locus_tag="KQD82_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4381186.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4798..5427)
                     /locus_tag="KQD82_002727"
     CDS             complement(4798..5427)
                     /locus_tag="KQD82_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381187.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5642..8299)
                     /locus_tag="KQD82_002728"
     CDS             complement(5642..8299)
                     /locus_tag="KQD82_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4381188.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8658..9602)
                     /locus_tag="KQD82_002729"
     CDS             complement(8658..9602)
                     /locus_tag="KQD82_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381189.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9595..10620)
                     /locus_tag="KQD82_002730"
     CDS             complement(9595..10620)
                     /locus_tag="KQD82_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381190.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10695..12263)
                     /locus_tag="KQD82_002731"
     CDS             complement(10695..12263)
                     /locus_tag="KQD82_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4381191.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12376..13287)
                     /locus_tag="KQD82_002732"
     CDS             complement(12376..13287)
                     /locus_tag="KQD82_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381192.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13271..14257)
                     /locus_tag="KQD82_002733"
     CDS             complement(13271..14257)
                     /locus_tag="KQD82_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381193.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14617..15018
                     /locus_tag="KQD82_002734"
     CDS             14617..15018
                     /locus_tag="KQD82_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG4381194.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15021..15419
                     /locus_tag="KQD82_002735"
     CDS             15021..15419
                     /locus_tag="KQD82_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG4381195.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4908 a         2724 c         1721 g         6218 t
ORIGIN      
        1 atttttagga ttctgttaca taatattatt taaatattct tctatatttc tcttcttctt
       61 cttcagtaaa cacattaaat acaactttta aatttttttc aggattttct tttagccata
      121 gtctaactct acttacagca agattagctg cctctttttt tggataacca aatacacctg
      181 tagaaattcc acaaaaaaca atatttttta tttcatcaat ttcttttatt gtatttaaac
      241 atgatttata acaatgaagc agttgctttt cttgctcttt acttggctga ccaccagata
      301 caataggtcc aacagtatgt actacaaatt ttgcaggtaa acaataacct ctagttatct
      361 tagcatctcc agtatattct aaatgccctt gtttttttat tattttgtca caatcttctc
      421 taagtctagg acctgcgcat gaatgtatct cattatccac gcatagatgt aatggttgta
      481 aacaccctaa taacttgtta tttgcagcat ttactattgc atctgctctt aaattcgtta
      541 tattacctct ccatatggca atcccttcct ctacttcttc tatatcattt acatctacta
      601 actctctttc ttcacattca atcaacaata aattatgtaa catattaagt atctctggag
      661 caatttcttt tggtggatat acattcaaaa gtcctctaag tatatctcta ggcttaattt
      721 catcatttga taaattgtat attttagaag gaacttcttt tgaaaaatat gcaattaaag
      781 tgtttatatt ttttcttctt tgttcgtcag ttagaggctt gatagtttta tcaaaatcct
      841 caaataaatt tacatcatca gcataatctc tccacttcat agccatctct ccattcttgt
      901 attttagact taaaaataat tatgtattta tgtacatatt tatttatttc tataaacacc
      961 tacataaatc ctgttaaaag tgtaaatttt ttattatgca acttctctgg ttatttctat
     1021 tgattgatag taaattattg tacctaaatt taatccaact aacaagtaac taaacctact
     1081 ttcctaactg caaaatacac tatgtttgcc acttgtatct catttgtaaa taaataaagt
     1141 ccctacaaat acattaacta ggctatgtac taaactgtag gaactttaaa aatatattat
     1201 tcttaattaa gttttggcca ataattttta tttgcttcta ttaaatcatc tagtaattct
     1261 tttgcaactg ttgcactagg aactgtctta gatagcataa gtgcttgcca caatttatta
     1321 taagacttct caatccaagc atcaactact aatttttcaa ctgctaattg ttgttctatt
     1381 aaaccttttt ggaatgttgg tattttccct attgataatg gttcataccc gtcttttcca
     1441 actatacatg gaatttcaac catagcatca tcttgtatat tttctatagc tccattattt
     1501 tgaactatca ataacatacg ttcctgtgta ttaaaagcta gagctgtagc taaatccact
     1561 ataaactctg catgtattcc tatcttaagc ccactgccct tagaatgtcc ttgcttttct
     1621 atcaatgcac atgaaccaaa aacttctttt tctcttccat caataacttc attggcacga
     1681 gtataattta catcagaatg atttacaaca taatctggaa atagatagta ctttaagtaa
     1741 ctacttggta ttgtagttaa atctgttttt actatttctt tagtaaataa attagtttca
     1801 aaccaactat ctttcttgtc atttttcatt ccttcttcac catttataaa accatattct
     1861 tttatatgtt ctactaattt tggtaaaagt tcatttcctt ttttatcttt aattgaagta
     1921 taccaaccaa agtgatttaa tccaaagtat cttatatcca tatgtttacg agatttgagt
     1981 cctgcaatcc ttgctatatt atgttccata cctattggca tatcacaaat atttattacc
     2041 ctagaattag gtctcaacct tctaacagct tccgctatta ttgatgctgg attagaataa
     2101 tttaacatcc aacaatttgg agaatacttt tccatataat ctatgttttc aagaacacct
     2161 tctatagacc tcattccata agctattcca cctggtccac aagtttcttg accaactacc
     2221 ccatgtttta gaggtatctt ttcatctagt tctctcattt caagttttcc aactcttata
     2281 tgagctaaac aaaaatctac atctgtataa gcctctttag ggcatgttgt tgctaaatac
     2341 tctatattag ggtccttttc ctttattagt atttcacaag ctttagctac cttattttgt
     2401 ctttcttcat cattatcata taatttgatt gctcttaatg gtaacctatc taagttatct
     2461 agcaacatta atatgatttc tggggtatat gtacttcctc ctccagctat tactactgaa
     2521 aatttttcca taataatatc tccttttcta tttaaaaatt tactgtattt ataaatatat
     2581 ttataaatac tagtacttta aagtaaaagg gagttcattt attaaatcta ttaatttaat
     2641 aaactatagc caaacttcaa gcactattta caagaaattt aattcttatt aattcttggg
     2701 tccatcatct caaatatatc tacaagttca gatactaaag aatgagcaaa taatgcattt
     2761 gaataatggt cttcagagtg cacaactaaa aaatcagtcc cactatcttc actattcata
     2821 gcatttgata gtaattctaa atgatacttt gatgctgttt gtattttctc tgaggctgaa
     2881 tttaaaaggt gttttgcttt attgtagtca ccttttttta tttccttaag agacatcatt
     2941 acatcacttt tagcatctcc tgcttctgat attatactaa atactttttc ttccatttca
     3001 ggtaccatat ccttctcctt ctaatgactt gttaaaaatc aaagctatca tcatctaatg
     3061 ttgtagctaa ttctgcattt ttatttaatt cttccttctc cattatctta aagaatggat
     3121 aatatatcaa ggtcataagt attatacttg caaatactaa tacgattgcc ttccaatcta
     3181 atgtcatcaa aaatgctgca acccctggag gtaaaaaacc tggtggttct gcaataggta
     3241 atcctactaa tcctactttt aatgccataa aattaagtgc tccaaccaat acaggtccaa
     3301 atacaaatgg tacaaacata tatgggttca taactattgg tgttccaaat attactggct
     3361 catttattcc aaatagtgat ggtaaaatca caacttttcc catactatta aaacttttac
     3421 acttacttct tgacataagg tacactaatc ccaaagttat ccctgaacca gttacttgca
     3481 agaaaccata gtaaaatcct attgtaaaca catattttgg gtctcctcct gctgctatca
     3541 gtgttgcatt ttctgccata tatgtagctg ctattggcat ccatatactt gaagttattg
     3601 ctgctccatg aagcccaaag aaccataata attgagttat aaatagtatt atagttactg
     3661 caaaaacatt atctacactg cttatagctg gtgctaagaa attcattata atagctggta
     3721 gtggttgacc tgagacattt ttagttattg ctgctattgc tgtaaataac attatacata
     3781 ctatcattgg agttaagttt ctaaaacttt tatttactac actaggtact tcttttggaa
     3841 atctaattac gagattttta tcattcataa acttagtaat atcaactact aaaagtgcta
     3901 ctaatatact tacaaataaa ccttgtgcat ctaaataact tgtatctaat acaccatcta
     3961 ctacttgtcc acatacaaca agttgtgcaa caactgctgt catacctatt tctacatgat
     4021 taagattata ttttttagct agtgcctgag ctataaaaaa tgctgaatat atagataata
     4081 aactcatagt aaatttatta gggataagta atgtatccat attttcagat aataatgttt
     4141 ttgcacttcc ttcaggtaac atattgttta atgccataaa taacatactt aaagaaccaa
     4201 caattattat tggggtaata cttatcatac cttcttttat agcttgaaga tgcctttgtt
     4261 tttcaatctt ttgtgctatt ggaagaagaa atcttcttaa attattttga agtgcttcca
     4321 tttttattcc ccccaattat ctatttttat ataattcgat tgccgacttc ataacagaag
     4381 gagcatccat acgaccataa tgcttagtat ctattaaacc aactgctatt ttgtaattct
     4441 tacaagcttc aactacatca tcttccttaa acctaatttg tggtgctaca agtactacat
     4501 ctgtatcatt agcttcaatg tatgatttta aattttcaaa tgatgttgcc tctatattta
     4561 tagttttagt ttttaacttt tcatgctcac tacaaaaact tttcatcttt tccacaagta
     4621 cacctgttga agctccattt gcacatacta ataaaatatt cataattacc ctcctacaat
     4681 atctaacctc ttttgttttc taatttttta tcagccaatt cttactctaa ttcctatcag
     4741 tttttacttt aattcttatt attttatttt aattcttagt aatatttact ctaattctta
     4801 gtactgccaa tgattaaact tgaaagccgt atctataact tcttcttgct tatatttttc
     4861 taatattttt aagtatcttt ctttataatc attatctact tctttttctg gtattactat
     4921 actagcttca tttaaaaagt ggaatgaatc ccttcccatt tctaatcctc tatatgtatg
     4981 cttgtcaagt gccacgtcca taacttctgg aactttaccc attgcaaatc cttttataat
     5041 tatatttttt aataaatcac ttgatctatc tttctcacat gcacaaagat atcttattgc
     5101 atgtatgaaa aataaaggtc tgtctccatc tgcatatgaa tattcctttc ttacttgatt
     5161 taaattattt atgattatta cagctgatgg gtcccccatt cctatatctt caactgatat
     5221 tgtaagtatc cttctccata gcttatcttc taattctggt gatgatatat atagttcata
     5281 agcaaattca caagcttctt ccaccatatt tcttcttata gctttttgaa atgacgatat
     5341 tatttcgtct gcactaaatc cgtttcttgt agtcattctt gcccatgggt catttgcctt
     5401 tttatctctt aatattttat cttgcatatt atttactcct ttttatttaa attttaatta
     5461 tttatattac tcatttaata atataagcaa gtattatgcc agttttttta gacactttaa
     5521 aacactgtta tttcaatcat ttttatcatc aaaacttaga tactttgtct aagtatattt
     5581 atatgtgtct aatattaata ttattttaga ctaaaaaaaa ttaccaaata agtttttata
     5641 ctcattggta atctttttcg attatttctg catattctaa tattaataat atttgatagc
     5701 actctttttc attaatttct atattatatt ttttatctaa ttgagatact atatctttta
     5761 ttttgcttag gctcttgtga taatcaagtg ttttttcttt atcaagcttt tcaatttctc
     5821 tagtatttcc aatcaaccca tctaccatga aagctaaatg catcataagt ccaattactt
     5881 tatcgtattt cttttctata ttaaatacta aaaacaattc atcagacaca tattttatat
     5941 ctttaaatat cctattataa ttagagcttt ttaaggatgt ctttaaacct tcaactacat
     6001 catcataacc tatacttatc tttataatat tttttaattt attgatattt tcatccttat
     6061 ctatatcttt tttgctaata tagtcaattc ctctaatatt aattctttgt tcattgatta
     6121 ctgcaacaat attactactt tctttaagtt tcgtaatatt taaacttaaa tctttttctg
     6181 ttttatatgg gacagatata atatctattc catctaagta cctactcatc ttttcttcta
     6241 atatttttct tgttttatta tctacacctt ctgaaacatg tgatgctata ataattgttt
     6301 tagacagatt ttctttattt tgaatttcat tagttccaaa atagtacttt ctcgtgttag
     6361 tgatagagtt gtatacgtca tttaaaggta atcccatatt ggccttctga actgcttcta
     6421 acacagttaa tgtcgtcacc ataggaatta ctcttactcc acactttttt tctttcttta
     6481 taatttcatc aaaagagttt aatgaaccca tatctgtaaa tagtaataca tctttattgt
     6541 ctatattatt aattaaattt ttaaaatcta caattacttc atcatagctt ttatctaact
     6601 tcatatcgaa agaaatagca ctgcactttc caataatatc accaactgct tttaccatac
     6661 tacttgctgt agtttctcca tgcatagcaa ctataatatt aatgttattc actattttat
     6721 tttgttcatc ttgtttagct tgtactatga ataatgtaat atatgctaca gtttctatag
     6781 ataaaaataa atcaaattca ttttcaatta ttccaactat ttttaatgct gttttaaatt
     6841 cacttctgtt atttcttcta atttgattca aatctatgtt gtttgtagag ctacttttat
     6901 ccaatacact ttttatataa attgccattg acttaaatat tgattcactt atatctattt
     6961 cttcatcttc tttaataatt tgtcttattg attcacacat gcttttaaac ttaacattat
     7021 taaatagaga agcatctgct atattcattt ctacagattt attaagatta gtatttactg
     7081 cattaataat atttatatca ttgtagttaa taacttcttc ttctcctaat gacgtaaata
     7141 caaaatattc tattccatgt gttataactt catctgcttt aatagacttc tctctataat
     7201 catcactcac ttcatttata gatttatcaa aatatttttt ctctatataa actggttctt
     7261 tattatctct tttatatctt aggtatccat ttgcaacagc taattttata tcattttgaa
     7321 gttgacctac attattttta caatcataaa gtaatagtga tttaattatt tctttatgta
     7381 cttttattga aatagaaata ctttttgatt cttccttgaa aaaatttttg actaattcca
     7441 ttctttcaat cattgtccta tctctcaatg atggtatttc tattttcatt ggtattcttc
     7501 tattgaatgt atctaatagt gttgatgtcg gattttctgt agtagcacat attattaata
     7561 ccttagactc tctttctttc gttgcatctc ctagtctttt aaatgttccc ttatctataa
     7621 aagtaaacaa catttcttga ccttctgctg gtagtctatg aatttcatct aaaaatagta
     7681 tccctccatt tgcaatttcc aaaagacctt ttctggaaga ttctgcacct gtataagctc
     7741 cttgttctac cccaaaaata tgtcccataa gtaattgtgc attattagca taatcagcac
     7801 aattaaatgt tacaaaagga gctttatttt ttctaattcc actatctata gaataatcgt
     7861 acatatatct agcaatcata ctctttccta ctcctgtttc tccatagata atagtatgaa
     7921 gtccatttgt aggatagagc attgcgacct tgcatttttc tattataggt cttaaactat
     7981 gttctttacc tattagtgtt tcaaaactat ctttttttat tttgttttct acttttgttc
     8041 ctttttttat gccaagcaac tcactttgtg tatcaagcaa ttcattcttg ttaaggttaa
     8101 gatttgcagg gatataatac acaggtcttc cttttatctt cgtaaccata ccctcattga
     8161 ataattggtt taaatatgta cttatattgt ttcttgcaat tcctgtcttt tctgataatt
     8221 ggttagctgt aatccctttt tcattttccc tatatgatga ttctaaaact ttcattactt
     8281 tatctttttt atatatcatt acaagctccc ctacattata gtattttatt aactaaaacc
     8341 cctatacaca ctttattttt taataaactc ttattcttta actaaggtta taatttatca
     8401 aactttcctt actttaaatt atagaaaatt attgtagaaa aaataagatt attgctcatt
     8461 ttttctacat tactattttt aattttatag tactttctta acaacatact ttacttgttt
     8521 ttaattttat tatatttgaa tttattatat ttgaaaagta gaaataaaaa accctatttt
     8581 ggagtagttt taaaatttct ccaaaaatag ggttttagtt tgtatttcaa ataaatacct
     8641 gttcaagtat ttttatacta aacaactttt aaattttgtt ggtatttatt tatgtctgat
     8701 ttagaagaat atacaaaatg tccatctgaa acctcaatcc aacttaataa cttttcattt
     8761 gaacaatcat atccattaga attatactct attctatttc ttgacttagc atatctaggg
     8821 tcaggaacag gaattgcaga aagtagtgat tttgtatatg gatgaatagg attattatac
     8881 aattcatttg aatctgccaa ttctactaat tttccattat acataactcc tatacggtca
     8941 ctaatatgtc tcaccataga taaatcatga gctataaata aacaagttaa attcttttct
     9001 tgttgcaact ttttaaataa atttactatt tgtgcttgta tagacacatc caaagaagct
     9061 ataggctcat cagctattat aaattctggg tctactgaaa gagctcgtgc tatgccaata
     9121 cgctgtcttt gtccacctga aaaatcagat ggatacttat ttgcataact tctatcaagt
     9181 cctactagct ttagcatctc atatacttta ttcattctat ctgttttatt tttacagata
     9241 ccttgaattt ttagtggctc tgaaattata tctgcaattg tcatacgtgg atttaatgat
     9301 gaagtagaat cttgaaaaat aatctgcata cttttgttta catctttttt tataaatttg
     9361 tacgtttttt tatctgatat gcaatttcct ttgtaaatta cttctccacc agtagattca
     9421 tgtagtctta ttatagtttt accagttgta gattttcctg aacccgactc tcctaccaat
     9481 ccaaatatct cacccttata tatatcaaaa cttatatcat caactgcttt tacagtagta
     9541 cttttgtcca aatgaaaata ttgctttaag ttttttattt ccagtatttt ttctctattc
     9601 attagaaatc acccttccac aatttactct aactggtaca tctacttcag gtgcatctgg
     9661 gtgcaataac caagttgctg cactatgtgt atcattaatt ttaaacattg gtggctcttt
     9721 ttcataatct atcttaagtg catttttatt ccttattgca aaagcatctc cttttggtgg
     9781 gttcaataaa tttggaggca ttccaggtat attatataaa tagtcatcct gcgaatctaa
     9841 agtaggtaat gaacccaaca acccccaagt atatggatgt ctagggtcat aaaatatatc
     9901 ctctacagtt cctatttcta caatctttcc tgcatacata actgctattc tatcagctat
     9961 agttgcaact actcctaaat catgagttat aaatattatt gacaaaccta ttttatgttg
    10021 taaatccttt attaaatcaa ttatttgagc ttgtatagtt acatctaaag cagttgtagg
    10081 ttcatcagca atcaatacat ctggattaca ggcaagtgct atagctatta ctatacgttg
    10141 tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat cttttctcag gattatcaat
    10201 tccaaccaat tcaataagct ctatggctct cttttttgct tcacttttac ttataccttg
    10261 atgaatgatt acagcttctg caatttgctt tcctattgat atagttggat tgagagatgt
    10321 catagggtct tgaaatatca ttgatatatt ctttcctctt atatcttcca tctctttttc
    10381 agacttctta accaaatcac ttgatttaag taaaacctct ccatttttaa tatatccatt
    10441 gtatgggagt atcctcatca aactcttaca cagtacagat ttcccacaac cagattctcc
    10501 aactatagcc aatgtctcac ctttttttag attaaaagat acatttctta cagcctgaac
    10561 ttctccttct tctactttaa aacttacaga tagattatta acctccaata aatgttccaa
    10621 aaaccatcaa cccctatgct attcaaacct tagggatata tttttatccc taaggtttct
    10681 aattaatttc agtattattc aattgtccag tcagctatgt tccagaatat acctacacca
    10741 tgatgaccta aaactgtatc aggtgttaat ccttttatgt ttggttttcc aacatatatt
    10801 gcatctatat aagcaataaa tgtataaggc atatccttag tcatttctac ttggaattgt
    10861 ttatataact ttaatttttc atctttatct tctgtttctc ttgctttttg aagtatttta
    10921 tctatagtag gattagagta agcactgtaa tttgcacctt tatctgttcc aaatacttta
    10981 tatgtatggt catctgggtc aaatggactt ccccaaccta ttaaatgagc atcttgatta
    11041 gcccaatctg tttcagttac aacaacagct ttagcatcaa caccaatttc ttttaactgt
    11101 tgtgcacata ttttagccat atcaactcta acttggtcac tctcacctgc tgttatctcg
    11161 aaagctaact tagtaccttc tttttcatag attccatcag aacctaattt ccaacctagc
    11221 ttttcaattt cttgtttagc tttttctgga ttatattcaa acttttctat atcaggatta
    11281 ttatatggtc ccatttgtaa aggtgaatat gctggtacac catgtcctaa taatacactg
    11341 tcaactattg cttttctatc tattgcatag cttaaagcat ttggtaatcc tttagctttt
    11401 ttatctttaa agaatttaga attaaaattg tataatatac ctctatagtc ggctgttttc
    11461 attatattta ctttaaaatt cttttcgtct ttttcaaagt tagacatatc tttaggagtt
    11521 atttgagcta ggtctaattc tcctgatttt aattgcattg ccttagcctt atcatctgga
    11581 acaatcttaa agactacttt atctaatcct ggttctttta caaaataatc actattctta
    11641 acaagtgtta tactttgacc tttgtcccat ttttcaagtt taaatggtcc agtacctatt
    11701 ggtttttgat taaattcatc agtagctata tccttacctt ccaaagcatg ttttggtaat
    11761 actcctactg ttaaataatc aagcattgct gtatttggag cttttaaagt aatttttata
    11821 gtattatcat taaccacatc aatttttgtt atatcttcat aatttgatgc tatctcagaa
    11881 gcattatctg gattcattat agtttctatt gtaaacttaa catcatttgc tgtaaatttc
    11941 tctccatcat gccacttaac atcatctctt aaattaaaag tatatgtatt tgttgcctca
    12001 tcaaatttcc agtcctttgc tagacaagga accaccttat tattttcatc atgagctgtt
    12061 aatccattaa atattaacga atttatttct ccatgttcat atagtgcagg atttatactt
    12121 gtatagtcat tacttccata aacaagtacc tttccatctt taggcgtatc tgccttctta
    12181 tctttgtctc ctccactaga acaacctgcc attagactaa gtatcattac taaacttaaa
    12241 acttttaact tctttaattt catgatgctc ccccttttgc tttttataaa atatttttca
    12301 aagctttgac cccatttatt taaatagata tttcaaatac ctcagtattt gcttatttag
    12361 tactcaaatg ctgatttata aattactaga ttttttatta ttattctttc ttatataatt
    12421 acctatattt gttatacaca ccaatgttgt aaccaaaaat atacccggaa tcagtataat
    12481 ccaccatcta tttgaaagta atgcttcctc tgacaaagat aacatacttc cccaagaaac
    12541 aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc gattcagtac caatagctgc
    12601 gcctatgttt gttacaacca tgaacattat tgatgccaca aaatttggaa gtaaatgttg
    12661 ctttaataca tagaagaatc ctcctcccat gctcttagct gctagtatgt attcgctatt
    12721 tcttatttgt ctaacttctg ttctcactat cttagatata ttcatccaac ttgttatccc
    12781 tattactatg gacatagata ctggatttga atttccaagt atagcttgta caaatattat
    12841 aattaatatt gaaggtatac taagtatgat ttctgtaaac ctcatcattg catcatctac
    12901 gagttcagat gctgaaccac ttatactacc ataaacaatt cctataacag ttgatattac
    12961 agtggaaaat agtcctataa ataaagatat ttttcctcca taccatatca tagaatatat
    13021 atctctaccc attgagtctg ttccaaataa aaaagtttta tttggagcta aattagaact
    13081 tgctaaatcc atgtatgttg gttcatgtgt cattataaaa gatgagaata cacttccaat
    13141 gactatgatt gataaaataa taattgatat atatggtaat tctttaattt ttttagataa
    13201 ggttttcttt gtttctatat gttcatcttc tgctactaaa atataatttt ctcctacaat
    13261 ttcaaaatca ctatattgtg gcttcattag tatcacctct atcatatttc attctagggt
    13321 ctattttatt gtttataatt tgagctaaca tattcccaaa tactactaaa gcccctgtta
    13381 ttaagcataa aactaataac atattataat catgatactt tgcactttca aaacaaagtg
    13441 ttcctaaccc aggataagaa aaaacttttt caactacata agttcctcct agtatatgag
    13501 gtatcgaaat ggccattata ctaatatatg atggcattat atttctcagg caatgcttaa
    13561 aaacaatagt tctattattt aaaccttttg ctttacataa aagcacataa tcttctctta
    13621 tttcctctag taatttattt cttatcatat atgtgtaata ccatagatgg cttaatataa
    13681 gtactgtaag aggtaagatt agatgtgata tcctacttaa tatactggac tcttctccca
    13741 ttgaataagc accactactt ggaagtatac ttaagtttat actaaatata agtatgagta
    13801 ctaaggcaac ccaaaatgat ggaatacagt tggttattgt tcctaatttg catatgattc
    13861 tatcaatgag cttatcttca tgtaatgtac aaaatattcc taacaataag gctagcacaa
    13921 aggtaagtat atatcctgaa ccacctaata ttatagtgtt tatatagaca tcatttataa
    13981 cagaagtaac attttgttta tatttaaatg aaattccaaa ttcccctttt gatgcatttg
    14041 aaatccattt tatatattga ctgtatattg gttcatccaa accaagtttt ttcatagcat
    14101 tttctttttc ttgtgtactc attctttcta ctccatcccc atagtaagag attaatgggt
    14161 ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga taataaaaac attactaaaa
    14221 taaactgcaa caactttttc aatatatcct ttaacaaacc ccaacccccc tttgttgcaa
    14281 taaaaaaaac cacataaaag caaacgtaaa gtaacgttaa aaccttcgtg gttccattat
    14341 cattaaacaa aattaaattt tctatattta tctatgagtt aattttagca tcatttatta
    14401 actgtgtaaa caaacttttt aaaaattgtt aaaatttcat caaattattt tatttatacc
    14461 taaaataaaa tttttatatt ctattcttca ttattttcta taaaattata aaattttagg
    14521 ttgactatat aagaaaagta tattatatta gtttgtatgc aaaccattta agaggtgatt
    14581 ttattgaaaa gtaattatga ctgttttaga atagccatgc tcttaaaaga actttattct
    14641 aaaactatgt acacagtaga agaaaatttt aaagaaaatg gattaacaca ccaacagatt
    14701 atagttataa aactgatagc tcataatcaa gaacttacaa tatcacaact atgtgatgaa
    14761 atgtctttag ctaaaggtac ggtttcaggc ataatcagca gattagaaca gattggatat
    14821 atagaaaaat tcaagaaatc taatgataaa agaaatacat acgttaagtt tacaacaaca
    14881 ggatttgaat ttgccacaaa ttttaaaatt aaaatgcaag aaagttttga tgatattttt
    14941 aaaaattgtg atgaaaacga attgagtgac ttagtaaaaa atcttagaaa tatattggca
    15001 aaagtaaagg agagataatt atgaattata aattaatact tgcaatagta tttataacat
    15061 cagctttaat tttctacact ataggtgtat ttggagaaag aaaggcaaaa attttaaaaa
    15121 agaaacatgt aattatattt tggcttggtt ttatatttga tactttagga acatttacaa
    15181 tgagtaacat agccaacagc catacttttg aagtaaaatc tgctttatca caaaaccttc
    15241 atagtattac aggtcttctt gcaattgtac ttatgctttt tcatgcaagt tgggctactt
    15301 ttgtcctata caaagatgat gaggaaaaga aaaaattctt tcataagttt agtatagttg
    15361 tatggactat atggttagtt ccatatttta taggtatgtt tattggtatg gctggatagt
    15421 aaaataaaaa gtgtgtaaga aatactgtag gtaaatttgt ataagacaaa tttatctaca
    15481 tccttctttc acacttttac atatttattt ataatactcc attatttctt tcatgtttct
    15541 taatacctat atagaatatt taattatttc t
//
LOCUS       DAENCV010000089        15329 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAENCV010000089 DAENCV010000000
VERSION     DAENCV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.090"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            52..1611
                     /locus_tag="KQD82_002736"
     CDS             52..1611
                     /locus_tag="KQD82_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4381196.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            1717..2676
                     /locus_tag="KQD82_002737"
     CDS             1717..2676
                     /locus_tag="KQD82_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381197.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGILLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            2679..3491
                     /locus_tag="KQD82_002738"
     CDS             2679..3491
                     /locus_tag="KQD82_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381198.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(3703..4605)
                     /locus_tag="KQD82_002739"
     CDS             complement(3703..4605)
                     /locus_tag="KQD82_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4381199.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            4960..6138
                     /locus_tag="KQD82_002740"
     CDS             4960..6138
                     /locus_tag="KQD82_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG4381200.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            6147..6803
                     /locus_tag="KQD82_002741"
     CDS             6147..6803
                     /locus_tag="KQD82_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG4381201.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            6803..7468
                     /locus_tag="KQD82_002742"
     CDS             6803..7468
                     /locus_tag="KQD82_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG4381202.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            7573..8349
                     /locus_tag="KQD82_002743"
     CDS             7573..8349
                     /locus_tag="KQD82_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG4381203.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            8411..9745
                     /locus_tag="KQD82_002744"
     CDS             8411..9745
                     /locus_tag="KQD82_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG4381204.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            10242..11168
                     /locus_tag="KQD82_002745"
     CDS             10242..11168
                     /locus_tag="KQD82_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG4381205.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(11561..15100)
                     /gene="nifJ"
                     /locus_tag="KQD82_002746"
     CDS             complement(11561..15100)
                     /gene="nifJ"
                     /locus_tag="KQD82_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG4381206.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         5402 a         2332 c         2369 g         5226 t
ORIGIN      
        1 tacaataaaa aatatgtgtt aaatattatt ttatggaggt tttataagaa tatgaaattt
       61 aaaaaattgg catcattaat cttagtttca tcattaatgc ttacatttac agcctgtgct
      121 tccaatgata aagacaagtc tagtggaagc aaatctggtg gaactgttgt aatacctatg
      181 gcaagtgacc cagatataat caatggagct tttgcaaacg ttaaagaaga ctctcttgca
      241 tcaaatatag tttatgctcc tctttatact tatgaaaaag gaaatttagt aaattattta
      301 gcagaaaaag tagattttaa agatagtaag gaattaacta taaaattaaa atccaattta
      361 aaatggcatg atggaaagcc aataactgct gaagatgtct tatttacatt taatacagtt
      421 ttagacgaaa aacaaaattc accatcaaga caatacttat tggtgggtga gaaaccagtt
      481 aaagtagaaa aaattgatga tttaacagtt aagattactt taccaactgc aagtgaatct
      541 ttcttatatg gtatatctaa aatttctcca ataccaaaac atgtgtttga aggagaatca
      601 aatatagcta agtcagaaaa aaataataat ccagttggtt ctggtgcatt caagtttaag
      661 gaatggaaaa aaggtgaaag catagtattt gaaaagaatg cagattattt tggtggagaa
      721 cctaaggcag attctatagc gctaaaaata attcctaatg aagcatctca agaagcagct
      781 ttaaataatg gcgaaatttc acttatgaag acatctgctg aaggatatga aaaggcaaaa
      841 tcaaactcaa acttacaaac ttatacttat agtgaagaaa gattaaacta cattgtattt
      901 aaccaaaata tatcaaacat ggctaacaaa gaagtaagac aagccttatc atatgcctta
      961 aatagaaatg aaatgataga atcagcatat ggaaaagaag gttctgtacc tgctaaatct
     1021 atattagttc cagaagcaga cttctataca gaagaaggtg ttgaaggata tgaccaagat
     1081 acaaataagg ctaaagactt attggataaa agtggtgtta aaattgataa attaaaaata
     1141 ggttataaca caggtagatt tgggcataaa aactatgcct tagtagctca acaagagttg
     1201 aaaaaaattg gtatagaagc tgaaatagtt ccttatgaaa gtaaggcatt cttcaatata
     1261 ctatttagta atagcacgga atgtgatatg tatgtaaatg gttatgcttg gggattagaa
     1321 ccaaacccat atagaggtat gtttgaaact ggtcaatatt gcaaccaaac taaatattct
     1381 aatgctgaaa tagatgcttt atgggaaaaa ggatttactg agttaaacaa ggaaaaacgt
     1441 gaagaaatat ataagcaaat acaacaggat atatctaagg atgcgcctat ttacactata
     1501 gattatgaac aaaatttaat ggcagctcaa aaaaatctaa aaggaataaa agatgctaaa
     1561 ccatctcctg caatattatt tgaagattgg tctaaactgt atgtagaata attgatgtaa
     1621 aattttatat tattaattag aaaaggagat gtctaaaaat aatattgttt tattatttta
     1681 aaacgagaca tctcttttaa ttaggagaat ttacttatgt taaaattaat attgaataga
     1741 gtaaaagttt taatacctat gttaatctta atatctatat tatcatttgg acttttagaa
     1801 ttagctcctg gtgacccagc agatgcatat ataaatcctt taatgtcttc acaggatatt
     1861 gaaaatataa gggttaatat ggggcttgat aaaccagtat atataagata tttaaactgg
     1921 ttgaaaaaca ctttaagtgg aaacttaggt atttcttata taaaccatat gcccgttaca
     1981 gaacaaatta tggaaaaaat gggaaataca tttattctta tgggtacttc tttgatattt
     2041 tcaatattgg tagctatacc tcttggaata cttttagctg taaataaaaa ttcaataact
     2101 agtaaagtat catctgtttt taattatata ggtgtttcta taccttcttt ttggataggt
     2161 atgatactca tttcaatttt ttcagttaaa cttaacattt tcccatcagg aggtatgcat
     2221 actattggaa atgatagtat tggagattta gtaaaacatt tagttttgcc tgtaataact
     2281 ttaggtctat ataatactgc tatttttaca aattatgtag aagctagtgt aaatgaacaa
     2341 ttaaaaaaac aatatgttat tacagcaaga gcaaaaggat tatcagaaaa agtcatacta
     2401 tttaaacatg tcttgaaaaa ttcactgaca tcattggtta caatcctagg catgagtata
     2461 caaaaattag tcactggtgc atttgttaca gaagtagttt tctcttggcc aggtatggga
     2521 agattaatga ttgattcaat tttttcaaga gattatactg taattatggc aataactatg
     2581 ttatcagcac tatttttaat tttaggtaat ttagttgctg atatactata cttattaatt
     2641 gaccctaaaa taaaatctag caaaggagga ttctagttgt gtttacaagt atcttaaaaa
     2701 aattaaaaaa agataaatcc tttgcttttg catgtattac tcttagcata ataatcatat
     2761 taattatagc ttcttttata ttaaatatag actctaatgc aattgatact acttctaaac
     2821 ttcaagaacc tagcttaaag catatctttg gtacagacga acttggtaga gattacttct
     2881 ctagagctct ttatggtgga aggatttcac ttagtgttgg tattttatca atgcttattt
     2941 ctgtagtatt tggtactgct gtaggtgtta ttagtggata tattggaggt aagctagacc
     3001 aattcttgat gagattagtg gatatgctta tgagtatacc aagtttctta gttttgattg
     3061 tattaaatac ttatctaaca cctaaagttt ccactattat actaattatt ggattcttaa
     3121 gttggatgga agtttcaaga attgtacgtg gcgaaacatt aaaaataaag gaaaatgaat
     3181 actgtctagc tgctaaagct actggtatca aaactagaaa tatactatta agacatatag
     3241 ttttaaactt aaaagagact attgtagttg ctggttcgtt aaatattgct aatgctatct
     3301 taactgaatc tgctcttagt tttttaggtc ttggaatcca attgcctctt gcatcatggg
     3361 gtaatatgct tcaagatgct caaagacata tttttgacaa gttatattta gctgtatttc
     3421 caggattact tatattttta acagtcatga gtttgactat aataagcaaa aaattttcta
     3481 gtaatactta acaaatttta tatagaaaat aaaaacagtg attatctaaa gatatacact
     3541 gtttttattt ttcgtttaaa aaaatacatc ttttgtatat tataacttta aatactactc
     3601 taccatgtta tgtaaagcct attattgttc tcaaacatat agatgaaaaa ctattagttt
     3661 attataataa aaattgattt atttgttatt ttaataacat ttttaagagt tctttttact
     3721 aaagaaatct ataataaatt taaatacatg ttggtctaca tggctataac gttctttttt
     3781 aggtctacat aatataactt tccatgtaat aggatgatta aaaggctttt ctataatttt
     3841 gctattttca aaaaaatcat gaactggtga tggtaaaatt gttataaaat cagaatttgt
     3901 ggttgatagc aacaaaaaat cccatgaacc agacattata tatcttttta acttaacatt
     3961 ttcatcagta aatttttgca ttagcttatg atgtatcata aaagtaggat taaatattgc
     4021 aagtaactgc tcattcaaat cgttccaatc tatcttatcc ttcttagcaa gtggatgatt
     4081 tatatttaaa aatgctgtaa gttcatcttc ttgaagcaca tgttcagtta ctacactttt
     4141 atcaatttta tgtggatgta acaatactgc ataatctaat tcctgtaaga taagcattct
     4201 atttaagtca tatgcaccct tctctactat atcaaattct atatctggat tatttgcaac
     4261 taactgtgca actacatctg aaaatgctat tccaagtata agtggaggta ttcctattct
     4321 aacttttcct ttaaattgaa ctgaatcttc tcttaaatct tccatcatat ttttgtattc
     4381 atttatgata tgttctgcat ttacataaaa tctttctcct gctggtgtta acccatttaa
     4441 cctgccttta tacctttcaa ataaatatac atcttcactt tcttcaaaat tttttataat
     4501 ttgacttaat gctggttgag atatgtgaag tgtcttagat gctattgata aattaaaacc
     4561 agatttcact atttcaacaa aatatttcaa ctgttttata tccattttaa acccccttcc
     4621 ccaaaagtat aatttagatt atattttttc ttctaatgat attttatagg aaaatcaaca
     4681 taatttcatt aatattttaa caaaatattt ttctaaatct actataaatt tttatcctat
     4741 aaatttttct tatacactat ataaaaaatt agacttagac aaaacgcttt tttcgccgta
     4801 atctataatt agaaacagga aaacctaaag cattaggaat aaaagtaatt gaaatactta
     4861 aaaacaagct tgtattaaaa aagtacttta ttatagtgtt ccctaatttc tttattcaga
     4921 aaatttttaa ttatcattca agaaaggtaa ctattagata tgaaagatgt agtaatcgta
     4981 tcagcagtca gaacacctat aggaagtttt ggaggtgtat ttaaaaatac ttcagctgtt
     5041 caacttggaa ctatcgctgt taaagaagcg atatcacgtg taagacttaa tttatctgaa
     5101 atagatgaag taataattgg aaatgttctt caaacagggc ttggacagaa tgttgctaga
     5161 caaattgcta taaatgcagg tataccaaat tctgttccaa gctacacagt aaataaatta
     5221 tgtggttcag ggcttaaaag tgtccagcta gctgcccaat caatcacatc tggtgaaaat
     5281 gatgtggtaa ttgcaggtgg tactgaaaat atgagccaag ctccttatat cgttccaact
     5341 gctcgctttg gtagtaaaat gggtgacata accatggtag atagtatgct aacagatggc
     5401 cttattgatg ctttcaatca gtatcatatg ggaataactg cagaaaatat tgcaactaaa
     5461 tttgaattta ctagagaaat gcaagataaa ttggcattag aaagtcaaaa taaggctgag
     5521 aatgctataa aaaacaaccg ctttaaagaa gaaatcgttc cagtagatgt tttaatacgt
     5581 cgaggaaaaa tagaaactat tgataaagat gaatatccta aattaggtat gacatttgaa
     5641 ggtttatcta aacttaaacc tgcattcaaa aaagatggta ctgtaacagc tggaaatgcc
     5701 tcaggtatta atgatggtgc ggctatgtta atactaatga gccaacaaaa agcagacgaa
     5761 ctaggtatta gaccacttgc taaaataaaa tcctatgctt cagctggtgt tgaacctgaa
     5821 gtcatgggta ctggtccaat ccctgcaact agaaaagcac tgaaaaaagc tgggcttagc
     5881 atcaatgata tagacttaat agaagcaaac gaagcgtttg ctgcccaagc attagctgtt
     5941 aaaaatgaac ttcaaataga ttcatcaaag ttaaatgtta atggtggtgc aatagcatta
     6001 ggacatccaa taggagcaag tggagctaga atacttgtta ccttaattta tgaaatgcaa
     6061 aaacgtaaag ttgaaactgg acttgcaaca ctttgtattg gtggtggtca aggaatatca
     6121 atggtggttt cacgatagga ggaaatatga ataaaatagt tagcattgat gaagctctat
     6181 ctcatgtaaa agatggagat acaataatgg ttggtggatt catggccaat ggttcaccag
     6241 aaaatttaat tgattattta tgtagtaaaa atataaaaga tttgacacta atttgcaatg
     6301 atactggctt tattgacaaa ggagttggca aaatggttgt aaataagcaa tttaaaaaga
     6361 taattgcttc tcatgtagga ttaaataaag aaactggaag acaaatgaac aacaatgaaa
     6421 cagatgttga gttagttcct cagggaactc ttgctgaaca aatacgtgca gctggttatg
     6481 gtctaggagg tatactcaca ccgactggta ttggtactct tgttgaggag ggaaagcaaa
     6541 aaattgttgt cgatagcaaa gaataccttc ttgaaaaacc aatttatgca gatgttgctc
     6601 ttctatttgc atcaaaggtc gataaagcag gtaatctagt ttataaaggc tctatgaaca
     6661 attttaataa tttaatggca tctgctgcca aaattacaat agttgaagca gatgaaatcg
     6721 ttgaaattgg tgatattgac ccaaatgaag ttaatacacc aggtattttt gtgaattaca
     6781 tcgttgaagg aggtaattta taatggataa attagaaatg caagaatata ttgcaaatag
     6841 agtatctaaa gaattaaaag acggagccgt tgtaaacctt ggaataggtc taccaaccaa
     6901 agtagcaaat tatattccgg ataatgtaaa cgttatctta caatctgaaa atggcttttt
     6961 aggtttaggt tcagccgaag atggaaattc aagtgatgaa actattgtaa atgcaggtgg
     7021 tcaaccagtt actatacttc ctggaggatg tttctttgat agtgctactt cttttggaat
     7081 aatcagagga ggtcatgttg atataactgt tcttggcgca cttcaggttg acaaatatgg
     7141 caatatagca aactatatga ttccaggtaa gatggttcca ggtatgggtg gagctatgga
     7201 cctagtaact ggagctaaaa aagttattgt agcaatggaa catacttcaa aaggttctgc
     7261 aaaaatatta aataattgta ctcttccact tactgcaaca aatgctgtag atttgatagt
     7321 tactgaaatg ggagttatgg aagtaacatc agatggaatt ttacttaaag aaataaatcc
     7381 agctttcaca ttagatgatg ttatttctgc tactgaggct cctttaatat tgtcagattc
     7441 attaaatgga aaaatatctg ttgtttaaaa taattaaaac tagaaaataa aaatctctta
     7501 gacgtattac atataaattt gtattgctta aaatattaat ataaaaataa aataaaattg
     7561 gaggaaatga aaatggttaa agataaagtt gtattcgtaa caggagctgc aagtggaata
     7621 ggtaaacaaa tagctgaatc gttcttaaaa aatggtgcta aagtaatgtt ctctgatata
     7681 aatcaagaag ctttaaataa taccataaca gagttacaaa aaaaaggtta tgattgcatg
     7741 agtgtaaagt gtgatgtaac taaggaagaa gaaattaacc acgctataga caaaactgtt
     7801 gaaaagtatg gcagattaga tatattaata aacaatgctg gacttcaaca tgtttctatg
     7861 atagaagatt tcccaactga taaatttgaa tttatgataa aaataatgct gacagcacct
     7921 tttatagcta ctaaaaaagc atttcctata atgaaaaaac aaggctttgg tcgtataata
     7981 aatatggcct caatcaatgg tgtaatagga tttgctggaa aggctgctta taactcagca
     8041 aaacatggac ttattggact aacaaaagtt gctgctcttg aagctgccga ttctggtata
     8101 acagttaatg ctttatgccc tggatatgta gatactccac ttgtcagagg tcagttcaat
     8161 gacctttcaa aaactcgtaa tataccattg gaaaatgtct tagaggaagt tttataccca
     8221 cttgtaccac aaaaacgcct attagatgtt caagaaattg ccgactatgc tatgttctta
     8281 gcaagtgata atgctaaggg tattacagga caagcttgct tactagatgg aggatatact
     8341 gcacaatagt tattatattt agggcttgaa gaaagtattc ttcaggtcct tctactaagg
     8401 agggatattt atgagtatta tcgcgccaat tggtataatt attggtatca tagctattat
     8461 atatttttca gttaaagaaa ttcatataac tatagctgct ccaatagcaa ctttaatagt
     8521 tgttatttta aataaaatgg atgttgtaac gtcaatgcta ggtgctgaca aaaacaatta
     8581 tatgggtgca cttggaaact atataatgag ttattttgca atattcttat taggctcaat
     8641 acttgctaaa tttatggaag aaagtggagc tactgtatca atagctgatt ttattttaaa
     8701 taaatttggc tctaatcatc cttatagagt tttagttgct atatttatag taagttttat
     8761 attaacgtat ggtggtataa gcttatttgt agttatgttt gcggtcattc cacttgcaag
     8821 aactctattt aaaaaacttg atatttcttg gaaccttatt cagataccac tttggcttgg
     8881 tattgcaaca attactatga ctataattcc tggaacacca gccattcaaa atgttatacc
     8941 tattcaatat ctaaatacgt ctcttactgc agctactata ccaagtattg taggtagtat
     9001 aggttgtgct acgtttggac ttgtctatat gaaatatgct ctaaacaaaa gtattagaaa
     9061 aggtgaaact tatgcaactt atacttcaga attagaagaa gaatctttag atagagaatt
     9121 accaggattt tttgcaagta tttttcctct attagcactc gtaattatag ctataactgg
     9181 aagtactttt ggttcagaat ttatgaaaaa aaatgtaatt tacatagctt taatagttgc
     9241 aattctttta tctgcttttt tattccataa atttctatca tctaagatac agacattgag
     9301 cataggtgct agtggttcta taggtcctat atttgctaca tcttctgcag ttgcatttgg
     9361 agcagtaata atggctgcac ctggttttga tgtatttagc aaacttataa tgtcaatacc
     9421 aggtagtcca cttataagtc ttactgtttt gacttcaaca atgtcagcaa taacagggtc
     9481 ctcttctggt gcattaggaa tagtaatgcc aaactttgca gattattatt taagtactgg
     9541 tctaaatcca gaattaattc accgtgttgc tacaatagct tcaaatatac taactatagt
     9601 tccacaaagt ggtgtttttc ttacattctt aagcttatct aagttaacac ataaaactgg
     9661 atttaaagaa actttcattg tagttgcagt tggttcacta atagcagaaa ttattgtaat
     9721 tacattagga ttactaatgt attaatataa aacaaattaa aattcataat atgaaaggtg
     9781 gtgtctcaaa gtgaactttt tacttaatga gacactcttt ttatatgaaa taacatatgc
     9841 ctatcattta aacaataaaa aagaacctta tctctatttt gagacagacc ctttttattt
     9901 cttaaagatt taaataatta taatttgttg ctaatacgaa agcatctact gaataaattt
     9961 aaccaaataa aaaaaaattt atcataacta taaatttacg ttatggaatt attataaaaa
    10021 tattattttc tctaactaag taattccata ctatacaggc ttttaataca aattgtcaaa
    10081 cacactgaaa tatacctatt tattccatat atatcttttt tacatatctt tacagtaaaa
    10141 aaagtcttaa tcttatgtta gtatagattc acgggtaacc ccctacacta aaggttacaa
    10201 atcagtaaca tttttagaat ttttagatta agaggtgttt aatgatgaga agaaatatac
    10261 caacaaaatt aaaaactata ttagctttag gagtgttaac tttagcaaca gcttcaagtg
    10321 ctgttactgc aagtgcttca tcacttgaaa ataatgtaaa tataagtaac aaaagtttag
    10381 tatatagtaa tactaaatac aagttttctg caaaaccaaa ttgttctgta aaaccagaac
    10441 ttaagcctga taaagataac tcttgtaagg ataagaatca taatgacaag aattgcaaca
    10501 acactaataa acctgaggac aacaataatt cagactctac taataagcct gataacaata
    10561 aacctgagtc taataaacct gaagacaaca ataattcagg ctctactaat aaacctgata
    10621 acaataaacc tgagtctaat aaacctgaag acaacaataa ttcaggctct actgataaac
    10681 ctgataacaa taaacctgag tctaataaac ctgaagacaa caataattca gactctacta
    10741 acaaacctga tgataataac aactcaggct ctacaagtga aagtttttca gcttaccaaa
    10801 aagaagttgt tgacttagta aatatagaaa gagctaaagc tggattaaat cctcttactc
    10861 ttgattcttc tatatcaaat gttgctacaa aaaaatctca agatatgatt gataacaact
    10921 atttttcaca caattctcca acttatggtt ctccatttga catgttgaaa aaatttggaa
    10981 taagctataa gacagcaggt gaaaatatag ctatgggtca aaaaactcct aaagaagttg
    11041 taaacgcatg gatgaattct gaaggtcaca gaaaaaatat aatgaatcca aatttcagta
    11101 aaataggtgt tggagtagca caaaaaagtg gtggttcaat ttattggaca caaatctttg
    11161 taggataaaa tactcttttt ccatacatat caagagagta tctctaagtg ataaaaaaca
    11221 caaatgagat actcttttgt atctatttta aaattatcaa atccaaatat acatactata
    11281 aatattattt gttctcttaa ttttagtgtg taaaacaata tataaaaagt attggcaaga
    11341 tgaaaatgga aaacttaaaa aagatattta ataggtaaaa acaaaatctt aatatcttca
    11401 taaaaaaagg ctgtctcaaa atagagataa ggcctttttc attgttgaaa tgattaaaat
    11461 atatttgatt tcatacaaaa agagtgcctc atgaactaaa aagctcactt tgagacactc
    11521 tcttttatca attctatata agcattataa tactttaata ttattcagct aattttttgt
    11581 atccttcata tctttcttta gcatcagctt cagctttagc aaataatcca tcagcttcct
    11641 ctgggaattg tttagctata gcagaatatc ttacttgtgc aagtatgaaa tctttgaagc
    11701 ttgcagttgg ttcttttgaa tctaaagtga atgggttttt accttcagct tttacttctg
    11761 gattgaatct gtataaatgc cagtatccgc aagcaacagc ttgtgcttcg ttagctacag
    11821 ttcttcccat accttctttt aatccatggt ttatacatgg agcataagct attattaaag
    11881 atggcccatc atgtttttca gcttctataa cggctttgat aaattggttt ttatcagcac
    11941 ccatagcaac ttgagctaca tatacgtttc catatgtcat agccatcatt cctaaatctt
    12001 tctttctaga ttttttacct gaagcagcaa acttagccat agcagcagct ggagtagctt
    12061 tagaagcttg acctcctgta tttgaatata cttctgtatc aaatacaagt acatttacat
    12121 cttcaccaga tgcaagaaca tggtctaatc caccgtaacc gatatcatat gcccaaccgt
    12181 ctccacctag tatccattga gatctcttaa ctagataatc ttttctatct tttatagctt
    12241 ttactaattc tttagctttt tcatctgtac aatcacaagc tggtttttct aataattcaa
    12301 caaccttagc acttgctact ttagaagctt ctccatcatt tctagaatct aaccactctg
    12361 taaatactgc tttagcatct tcacagatat ccatagaaac taactctgtc atagcatcta
    12421 ctattttatt tcttatttgc ttaacagctg tgtacatacc aaatccatat tcagcattat
    12481 cctcgaataa agaatttgcc caagatggac ctttaccttc atggttacaa gtataaggaa
    12541 ttgatggtgc agaagctccc catatagaag aacatccagt agcattagct atcatcattc
    12601 tatctccata taattgtgta actactttaa tgtatgcagt ttctccacat ccagcacatg
    12661 ctcctgaaaa ctccattaat ggttgtctaa attggcttcc tttaacagtg tttgcaggca
    12721 taacatcacc ttttggagct acctcatcag gatttactgc aaatgcccaa ttatctaact
    12781 ctgtatcaag ttgagtatct attggcttca taactaaagc tttttcttta gctggacata
    12841 tgtcagcaca gtttccacat cctgtacagt caagtgggct aagttgcatt ctatatttta
    12901 atccttctaa tcctttacct aatgcttttt tagcttcaaa tccttctgga gcattagcta
    12961 attcttcttc agtaactaat actggtctta tacatgaatg aggacatata aaagaacatt
    13021 ggttacattg aatacagtta tctattatcc attcaggaac atcaacagct ataccacgtt
    13081 tttcataagc tgcagtacca caagggaatg ttccatcttc tataccattg aatgtactta
    13141 ctggtaagtt gtttccttct tgtgcagtca ttggtcttag tatatttttt ataaattctg
    13201 gttctttaac ttctccttta tctgcatcaa cagcattttc ccaagaagct ggaacttcaa
    13261 ctttaactaa agcatttatt cctgcatcaa cagcagcata gttcatgtta actatttttt
    13321 cacctttttt accataagct ttaactatag aatcttttaa gtatttaaca gcatcttctt
    13381 ctggtattat ttcagctaat ttaaagaatg cagattgcat tatcatattt attctgtttc
    13441 caagacctat ttcttgtcct agtttaacag cgttaactgt atagaatttt atatcatttt
    13501 tagctatata ttgcttcata tgagctggta aatgttcttc aagtcctgct tggtcccata
    13561 tagtgttaag cacaaatgta ccaccttttt taagaccttt taataaatca tattgataaa
    13621 catatgattg tttatgacag gcaatataat cagcttcatc tattaaataa gtagctctta
    13681 ttggagtatc accaaatctt aagtgagaca tagttatacc accagatttt ttagagtcat
    13741 aatcaaagta agcttgagca tatttttcag tatggtcacc gattattttg atagcttgtt
    13801 tattagctcc aacagtaccg tcagaaccta atccccagaa cttacatctt atagttcctt
    13861 tagaagctat ttttattggc tcagatggag ttaaagaagt attagttaca tcatcaacta
    13921 tacctactgt aaatccattt ttaggttgtt ctgaaactaa gttatcaaaa acagttttta
    13981 tatctgaagg agtagtatct tttgaaccta atccgtatct tccacctatt atcataggag
    14041 cattttcttt tccatagaat acatcacgta catctaagta taatggttca ccagttgaac
    14101 ctggctcttt agttctatca agtacacata ttctttcaac agtagatggc atagcatcta
    14161 agaaatgttt cattgagaat ggtctatata aatgaacttt aactaaacca tatttttctc
    14221 ccttagcatt taagtaatct atagtttctt ctatagtttc tgttactgaa cccatagcta
    14281 ttaatacata tttagcatct tctgctccat agtaatcaaa taaactatgt tttctacctg
    14341 ttaaattact cattttttcc atgtattttt caactatacc tactatattt tgatagtatt
    14401 tattagaagc ttctctagtt tggaaatata tatctgggtt ttgagcagta ccacgagcaa
    14461 ctggatgatt tggagataaa gcattatctc taaatgcttg aactgcttca aagtttaata
    14521 aacttgcata atcttcattt tctagtaatt caactttttg gatttcatgt gaagttctga
    14581 atccatcaaa gaaatgtata aatggtaatc taccttctat tgcagctaaa tgtgcaacag
    14641 gtgctatatc agctacttct tgaactgaac cagaagctaa taatacacat ccagtttgtc
    14701 tagcagccat aacgtcttgg tgatccccaa atattgataa cgcttgagat gctaaagcac
    14761 gagcacttac atggaatact cctggtaaca actctcctgc aactttatac atgttaggta
    14821 tcattaataa taaaccttga gatgctgtaa aagtagaagt taaagctcct ccttgtaaag
    14881 aaccgtggaa tgttcctgat gcacctgctt ctgactgcat ttcaacaaca ttaactgttt
    14941 gtccaaatat attttttctt ccttgtgatg cccactcatc aactacttca gccatagttg
    15001 aagatggtgt gattggatat atagctgcta catctgtaaa cgcataggca acatgagctg
    15061 cagctgtatt tccatcaagt gttttcataa acttagccat aatatgtatc ctccttgata
    15121 gctacaattg tacttgttta taacaagtct atttttttat atttacatat aatttacaca
    15181 acaaaaatat aaacacacat atagtcgcgc aaatactcta tcttctaata taatactaca
    15241 gattagataa aattcctttg aaaattgtaa attaattaca tatttaagta gatttttttt
    15301 aaaattaaat atgttaagtt tttttcata
//
LOCUS       DAENCV010000090        15085 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAENCV010000090 DAENCV010000000
VERSION     DAENCV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.091"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            30..953
                     /locus_tag="KQD82_002747"
     CDS             30..953
                     /locus_tag="KQD82_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4381207.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1125..2267)
                     /locus_tag="KQD82_002748"
     CDS             complement(1125..2267)
                     /locus_tag="KQD82_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG4381208.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMDEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2285..4039)
                     /locus_tag="KQD82_002749"
     CDS             complement(2285..4039)
                     /locus_tag="KQD82_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG4381209.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4175..4381
                     /locus_tag="KQD82_002750"
     CDS             4175..4381
                     /locus_tag="KQD82_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381210.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4386..5309)
                     /locus_tag="KQD82_002751"
     CDS             complement(4386..5309)
                     /locus_tag="KQD82_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381211.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKTIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5563..7263)
                     /locus_tag="KQD82_002752"
     CDS             complement(5563..7263)
                     /locus_tag="KQD82_002752"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG4381212.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7675..8088)
                     /locus_tag="KQD82_002753"
     CDS             complement(7675..8088)
                     /locus_tag="KQD82_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG4381213.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8154..10532)
                     /locus_tag="KQD82_002754"
     CDS             complement(8154..10532)
                     /locus_tag="KQD82_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG4381214.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10851..12302
                     /locus_tag="KQD82_002755"
     CDS             10851..12302
                     /locus_tag="KQD82_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG4381215.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12506..15019)
                     /locus_tag="KQD82_002756"
     CDS             complement(12506..15019)
                     /locus_tag="KQD82_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG4381216.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
BASE COUNT         4815 a         2610 c         1780 g         5880 t
ORIGIN      
        1 aaataagtta atattgagga gggataatta tggaaataag agtattaaga tattttttag
       61 caattgcaag agaagaaagt atatctggtg cggcaacctt tttacatata acacaaccaa
      121 cactttctag acaaattatg gaacttgaag aagagttggg agttaaatta ttggttcgag
      181 gaaatagaaa aatcacctta acaaatgaag gtaggctttt acataaaaga gcagaagaaa
      241 ttatatcatt agttgataag gttgaattag aatttgacaa tcccaatgag attattagtg
      301 gtgatatata tataggaagt ggtgaaactg aagctatgcg acttatagcg agaactgccc
      361 atcattttca tcaaaaatac cccaatgtaa gatatcacct ttttagtggg aatgctgatg
      421 atgtgactga aaaattagac aagggactgc ttgattttgg tattttaatt gaacccgcaa
      481 atatacagaa gtatgattat gcacaaattc ctgctactga tgtatggggt ttactaatgc
      541 gtaaagatag tgcccttgca tcacaaagca caataaatcc aaaagattta tgcaatatac
      601 ctcttattat ttcaagacag acacttgttg ataaagcaat ttctaaatgg cttcaaggca
      661 gtaatgaaaa actaaacatt attgcaacat acaatttagt ttataatgct tctcttttgg
      721 tagaagaagg tctgggctac gctctgtgtt tagatagact cgtcaatact acagaaaaca
      781 gtaatctttg ttttagacct ctatctcctc ctttagaagc taaattaaat attatatgga
      841 aaaaacatca aatattttca aaggcagcag aaaaattttt acttcaaata caaaaagatt
      901 tttcttctat catgttggat acatctaagt tggatacacc caaagaaaaa taaatcataa
      961 taaaaagtga gagtgtctca aaatgaactt tgcactgtat aaggcactct tttttataca
     1021 aagtaaaata tatgttcatc atttcagcac taaaaaagag gcttatctct attttgagat
     1081 agccccactt aaccaataaa attaaataca acttaacaat atccttactt atttattcca
     1141 agttctctta gacaagcaat agtaatttca tcttgtggat ttaattcata agctctttct
     1201 atatagtaag ttgcatcatc aatatcacca ttatttaaat aagccattcc taaattgcaa
     1261 agtatttctg ggtcttcctt tatcttagcc gcttgttcaa aatactctat tgctttatct
     1321 aaattattct tataagcctc acaaagcccc aattccacta tagtatccac ttgattaggc
     1381 tttattatca atatcttctc aagatacatc ttagcctgct ctatttcgtc cataccctta
     1441 tatccaagtg ctatcataaa cagcaagttc caccaatcca tatgttcttc ttctaatgga
     1501 agtaacttct ctaatccttc cttaaactta ccttggaata caagtgtata tccttcttca
     1561 tattgaactt taaagtccat cttgcccaga ttctcttgta cctcagctac taaatcagca
     1621 tctaacccta gccttaacgc ttcttcccaa gtcagcttag tctttaaata ttgaccttga
     1681 ttataataat ggtagcctaa gtgataataa cctaatgcaa aattctcatc aacatcaatt
     1741 accttttcaa gcttagccat ggcttcaagt aagaatgcat tcattgcctt atcatccata
     1801 tctttttgat actgtttagc tatctcttgg caaacaatcg catagttata cattgcatct
     1861 aaatcatctg gatacattgt tataaatgct tttatatata ttaaagaatc tgtatgttca
     1921 ccaatctcaa agtattttct cgacatatat cccatatacg atttcaaatc caaatcaata
     1981 tcttttgata aagcatttaa aaacttctca tactctgcat tgtttttaaa cttactatct
     2041 attccaatta tatatatcat tgcatctgct atagacattg aatttaactt atcttgagca
     2101 gtcttttctt ttatcccttt tactaatacc tcatttttta taggtacatc taatccacct
     2161 tttggcactt catagctttt taatttaaac tctccaccat gttttatagt tataaaagct
     2221 aattcttctg ctttttttag caaatatttt tctatcttaa aatccatttt atcctcctaa
     2281 atacttaaat ctctacttta tgagttttag atttctcaaa tattttttta tcatcatact
     2341 caaataagac tacattttct acttctctta tttctgtatt tatatcattt tttaaatcca
     2401 caacattaaa tctaaatggc tcaaaatgaa catattttat tatatgttta gctggtttat
     2461 caacaaaact aggtaaatat gttgataagt tatcattatc ttgtaaaaat acatggcatc
     2521 tattcttaaa atcatccaca tctagcttat taaaaaactg tggtatattt gatgttttac
     2581 ccagtgatat atagtcatat tttaatatat cgttgaatac atcattatct atgttgattt
     2641 tttcattata gaaatccatc aatattttat ataactgatt ctttccttgt gccaaatcaa
     2701 agtagccatt ttcatcaaaa tacttggcaa aaacctcaaa gaatttaaaa gggctttgtt
     2761 tataaaatct gcctataata tatctcatag ataacacaaa gtttttagaa ttgtagtatc
     2821 tctccagtat atcttctata tcctttagct tcaaagtttc ttcatagctt atataattat
     2881 tgtataaaaa ctcatatgga gcataatcct tatatttaaa accatgctca tctgcaactt
     2941 ttctcatacc tgtcccttta atcatcttta aaaagcctaa ttgcagatgc tctataccta
     3001 gattaaatac atcattaaaa gaattttcaa agctcttata atcttcataa ggcagtcctg
     3061 caattaagtc taagtgctga tgtatatttc tataagatgc tacagtttgt acaacatgag
     3121 acaacttttt aaaatcatcc cttctgccta ctgcatcaag tgttttttca ttcgttgact
     3181 gaacccctat ttcaaattga aaaagacctt ctttacaatc agccaaaaac tcaagcatct
     3241 tatcatctat caaatacgct gtaacttcaa aatgataagt tgtatatcca ttatcatttt
     3301 tcataaggaa atccatgatt tccatagcca cttttctatt tgcattgaat gtcctatcta
     3361 taaactttat ctgtgatact cttgcatcta ttaaagcttt taaatcactc tttatcctgt
     3421 ctaaactaaa ataccttaga cctggaatag tagatgataa acagtactgg cagttaaaag
     3481 ggcaacctct tgatgattca taatacacta ttctattttc atattctttt ggattcaagt
     3541 tttcataagg actaggaatc tcatccaaat cttgaagtaa ttccataggc ttattcacta
     3601 ttatctcatt gccttgtcta tatacaagtc catttacatc atttatttcc atctttcctt
     3661 gcaagtgaag tactaagtct ctaaaaatta attctccctc tccataaagt atataatcca
     3721 caaaatcata ctttttcatt gcctcataac tatcataact cacttccggt ccgcccaaag
     3781 ctattttagt attaggtcta atttttttta gattatcaca aagtttaact atatctccca
     3841 cattccagat atatgttgag aataatatta tatcatattc atttttatat atatccttta
     3901 aaatatagtc caaatcgttg tttatcgtgt attctttcat attaacttct atcaaatctc
     3961 ttacaaattc ttttagatat cttatagcca aatttgtatg aataaacttg gaatttaatg
     4021 ttgttaataa aattttcaaa tatttctcac ctctttttaa gattatactg tctttttgta
     4081 ccccttatag gttaaaatag tatatgtaaa gtctaaataa tatctatata attaatttat
     4141 taatttcaag taatgtgagg aaaggaagtt ttttatggaa ttaattgaaa aagtatctat
     4201 caactcttta tcaaaaagag atttactact tattataaag tctcttgaat acactaatga
     4261 aaatactcaa attagtgatt tcattgacct taggaataat atagtaaagg aattatgttt
     4321 tttaactgac actaaggaaa aagatttttt agattatcta gaaaaaaata gcacactcta
     4381 ataacctaaa cctttttaaa ctttaatata taaatacctg atttagaatt tatttttatg
     4441 cttaatatat tatcattaaa ggttttcgac ataactgtta tttgattaaa tatagggtct
     4501 actatagtat taccttcttc aagtcttata agacttcttg ggtctatgct tttattatat
     4561 gccttaactc caattttata ttttctcaat ttaacaacat attcggacat agaaaaaata
     4621 aaatctatat tatcttcatt taatttacta tcttcaccca aaatggataa tatagatttt
     4681 tttatgctat taattccttc attttctaca gttctatctt ccatatatac atcatataga
     4741 acatttaaaa catttaataa aatactttct tcatcataat cttcagattt caaatcaatg
     4801 tacttatcaa ttaattgtat tgctttaatc cttgccatct gaaattttac aacatctgat
     4861 ttgtctaatt ctatagaaaa tcctattgtc ttgtctttaa tactctgtag taattcgaca
     4921 tactctattt tactattatt tataagattg tgcataatgg aattatacac tacagaacct
     4981 aataaataat caaataaaag ttcttctttt ttaaaataaa taacatactt ttttattagt
     5041 ttaataacct catcttcact tttttgaata ttagtatttg ataaaattag tggtaataaa
     5101 atatttaaaa aattgatatt tcctattttt tctccataaa atctatagta aataagttca
     5161 tctctcagtc tattttcgtc tatatacaca catctttgcg ttttatcatc tattaaggat
     5221 tttgtattta ttatagttaa atctttaaga gccctacttg cactactaaa agaagatgtg
     5281 tagacttttt ttagtaattc gtcattcata aatatatcct ttctgaaaaa ttgaatgtaa
     5341 ataaattgtt attaaaaaaa aagagacagc ccgtttaaga agctgtcata tataggggga
     5401 tagtagtgta caaaaatcat tgtaatctac tatctatata ttattatacg atactacttt
     5461 tttatatgta aagcatcagt tttcgacatc cctatatttt acagttatac aaattgcgta
     5521 ctaagttcaa ttttattctt tttatttcaa cattttatca tgctacattc tttcaggagc
     5581 gtgcattccc aacaatttca gagaattttc taaagtttgt tttgttgcta aaactaatgc
     5641 aagtcttgct tttcttattt ccaaattttc tactaaaatt ggattgtcat gataaaattt
     5701 attaaacgct tgagctaaat ctagcataaa tctagtcact atatgtggct catttttctt
     5761 tagagcaagt aaaatattct tattgaatga ttctataact ctaagaactt ctgcactatc
     5821 ttcatcagct aatagactat aatctatatc agaagtcact tctacttctg cttttcttaa
     5881 aactgcacag catctagcat gtgtatactg aacatatggt ccagtctcac catcaaaact
     5941 aagcgttctt tcccaagaga aggtatagtc tttaattcta ctattagata actcttggaa
     6001 tactactgcc ccaacaccta cttgcttaga tatttcatcc acatttttag catttgggtt
     6061 tttagctagt ataatttcct tagttttatc tatagcttga tttaaagcat cttctaaaaa
     6121 tactactctt ccttttctag tcgacatagt accttcttca agtgcaacca taccaaatcc
     6181 aacatgaatt aaatcttttg cccaatcata tcccataagc tcaattacct taaaccattg
     6241 ttggaaatgt aagttttgtt gagaaccaac tacatatata cacttgtcaa aatcataagt
     6301 ttcttttcta tatattgcag cagctaaatc tcttgtcata tataacgttg aaccatcatt
     6361 cttagtaata agtgctggtg gcattttata ctcttctaaa tcaacaattc ttgctccttt
     6421 agactctttt aaaagatttt tctcttctaa tagttcgata actctgttca ttttatcaga
     6481 ataaaaactc tctcctgcta aagaatcaaa ttctatatct aaaagtttat aaactctgtt
     6541 aaactctttt aaactctcat ttctaaacca ttgccataat tcttgtgctt cttcgtcacc
     6601 attttctaat ttattaaacc atgctcttgc ttcatcttcc atttctgggt gttgttcagc
     6661 ttcatcatgg aatcttacgt ataattttag caattctgga attggattag actctactac
     6721 ttctttttct ccccactttt taaaggccac tattaactta ccaaactgag ttccatagtc
     6781 acctaaatga tttattctta tagtcttgta tccttgcgaa tcatatatct tatataaagc
     6841 attacctata actgtagttc ttatgtgccc tatatggaaa ggttttgcta tatttggaga
     6901 agaatattca actattacag ttttatcttt tccaaaatca ctatgtccgt aattttcttt
     6961 ttcgtctaaa acttttttaa ttacagtttc tgctaattga gatttattta caaagaaatt
     7021 tacatatccg cctaattgta ttacttttgt tatctctcca cttggttcta tactttccgc
     7081 caattcactt gctatcatgt ttggagcttt tctaaacact ttggctaatt taaagcatgg
     7141 aaaagcatag tcccccatat ccttatttgg tggaacttct ataagtgcct ctatctcttc
     7201 tttcgataaa tcttctattt tttctttaag gcaatttgat attgccacct taaaatcttg
     7261 cattctgttg ctcctctcta cattgttcct ggtttatatt gtgcttatct ctttatgtta
     7321 aatcaaattt cgtgtttttt caagtatttc attacagtgt aaaacacttt agtaattatg
     7381 ttttctgtat atattaaaaa tactataatt gcagttttta atcacactat tattgatttt
     7441 ttataagact tttttctatt ttattgtttc ttgtactatc cagttgactt tatatacttt
     7501 ttttaatctt ttatctattt tcatacgtca atgctaattt tatatacttt tcttttaatc
     7561 ttttattaat tttcatacat caatgttaac tttatatacg tttttttaat cttttatcta
     7621 ttttcataca tcaatgtcag ctttatatac tttttttact cttttatcta ttttctataa
     7681 atctttttca ctcttaactt ttattccttc ttcttctaat agccttgctg ttataccttt
     7741 ccctggtatc tttttacctg aaaaactacc atcatatatt agattacatc cacaagaggg
     7801 gctaccctct tttagtattg cttcgctaca tacatataaa tttgctattt ttagagtttc
     7861 attagcccct cttacaaagt tttctgttac atccttatta tctttagtaa ttacttttcc
     7921 atcaactatc tcagcaggtg gtcttggtgt agatagacca cctaactgct ctgggcatac
     7981 aagtgtaaat tctttgcctt ttaaatattc cttgactttt tcattatcat tattatcgcc
     8041 actatattta caatttattc ctattaaaca tgcagatacc aatatcataa aaatgtgctc
     8101 ctatacaaat tatatatatt ctcatacaaa ttactaatgt attcttatac aaattatttt
     8161 aatttaacaa ttgttactcc tgctccacct tctccatatt gaccacctct ttgtgatttt
     8221 acatgtctat ttcttcttaa aatgtcttgt agccctgctt ttaatactcc tgtacctatt
     8281 ccatgtatta ctgttacaga ctctaaacct gccacatatg catcatcaag atacttttct
     8341 acttccatta tagcttcttc taggtttaac cctctaaggt caacttcatt ttttacacta
     8401 ccagattttg accttataat gtttctcgtt gatttagtta catttgttga aacatctttg
     8461 cgtgtttttt gtaaagactt aaaaggtaga gtcatcttca ttataccaat ttgcactact
     8521 gcttcttttc tctttttatc tactgaaact acactaccat tttgatttaa agttattacc
     8581 ttaacttctt cacctggctt taagtctttt atctccttat ttgatacctt tggaactatc
     8641 atacttttta ctgttggctg aagacttccc atagagctgg tcaattcttt tctaagttct
     8701 tctatcttac gattcttttc ttttgaagct ctttcttgtt caagacttct aagttctttt
     8761 attataatat ctacttcttc tttagcttgt cttataattg aaaatgcttc tgatttagct
     8821 ttttcaatca tcttatctct ttgactgacc aatttttcaa gcttttcgtc atactctact
     8881 ttcagttttt caatctcttc ttttaatctt tcagcttctt ctctatcttc tactgctttg
     8941 attctattct tttcaacatt ctgtaacaca tcttctaaag ctatgttttc tgtatttata
     9001 tattctttag cacgtgaaat cacataatca cttagcccaa gttttctaga aatctcaaac
     9061 gcatttgatt ttccgggaac acctataagt aacttatatg ttgggcttaa agtttctata
     9121 tcaaattcaa ctgctgcatt ttctacacct ggtttagtaa gagcataatt tttaagctca
     9181 ctatagtgag tggtagctat acatttagct cctacagaat ttatatcttc taatactgct
     9241 attgcaaggg cagctccttc aacagggtct gttcccgctc ctaactcgtc aaatataact
     9301 aaagagtctg ctgttacgtt ttgtaatata gatactatat tcgtcatatg agatgaaaat
     9361 gttgatagac tctgttcaat actctgctca tctcctatat ctgcaaatac attatcatat
     9421 acacacatac tacttccata atcagcaggt atatgaagtc cactttgtgt cataagtgca
     9481 aatagaccta cagtttttat tgtaacagtc ttaccacctg tgtttggacc tgttatcacc
     9541 aatgtatgga agtctcttcc taaataaatt gtatttgcta ctaccttctt tttatccagc
     9601 agtggatgtc ttccattttt tatgtttaaa tatttatcct catttaaagt tggttctatt
     9661 cctctcatct gtatagaaag ttttcccttt gaaaatgcaa agtccaatct tccaagtatc
     9721 tcttgattag aaattaagtc ttcactaact tctccaacca tagcagaaag ctctgataaa
     9781 attctttcta tttcttcttg ctcacctagt tttaactgtc tcaattcatt gttcatctct
     9841 actatagtca ttggctctat aaaaagtgtc gcaccagaag atgactggtc atgcactata
     9901 ccagctactt gcgacctata ttcagatttt acaggtacta caaatctatc acctcttaga
     9961 gaaattattg catcttgtaa gtacttttga taagtagttg aacttattat tgaatttaat
    10021 tttgacctaa tggattgatt tttttgggct attcttcttc tgatatctct aagtgtactt
    10081 gaagcattgt cagatatttc tacttcacta attatagcat tgtatatctg gtcttctatg
    10141 tccttgtata catatagaga atttgataga gattgtatta ttggataatt aaaatcttct
    10201 tcatcactac tagataagct atttcttaat cttctagcca ctcttaaggt atctgctatc
    10261 ataatcagac ttcctgggtc aagagaagca cctatataag ctctctttac cttatcttct
    10321 atatcatgaa taccttccaa ccctactgaa cctctcttta taataattga ttgagcttca
    10381 ctagtttctt ctagcataga tttcacttct acaaaatcag tatttggtac taaattttct
    10441 atatatttta gacctaaaga agatgaggct ttcttcttta acaaatctat tattttattg
    10501 tattctagaa ctcttaacga tttctcattc attttctata ttcaccatcc tcgatatttt
    10561 aactttttta tttaatttta aaaatgtatg ttttcaagca tttccaataa aaactgcaag
    10621 ttttatttct ttttcatttc taaaattatt atattttttc tcttttagat atattttaca
    10681 catattttgt attatactat gtatgtagct atctatatat tatacataaa catgctttat
    10741 ttgtaatata atctgttaaa aaatcttctt taatattttt aaaggagttt attttagctt
    10801 ataaaaaaaa atagctactt atatagataa attaaggagg aacatgagtc atgggaaacg
    10861 tattagaagg attaaaacca gaaagtgttt ttaaaaactt tgagaaaata tctcaaattc
    10921 ctagaggttc tggaaatgaa aaaggcataa gtgatttttt acttagcttt ggtaaaaatt
    10981 taggtcttga aactattcag gatgaaagct taaacataat aataagaaag cctgctacaa
    11041 aaggatatga aaattgtcca ggtgtagttc ttcaaggaca tatggatatg gtttgcgaaa
    11101 aagaaaagaa tgttgaacat gattttctta aagaccctat aaaattaaga attgatggtg
    11161 acatgatata tgctactgga actactctag gagctgacaa tggtatagct gttgctatgg
    11221 gtatggctat attagaagat aatactttag agcatcctgc actagaagtt ttagttactg
    11281 ttaatgaaga agatggtatg aatggtgcag atgcactaga ccctagctta attaaaggtc
    11341 aatatatcct aaatatggac tccgaggagg aaggatactt attagttagc tgtgctggtg
    11401 gtaaaacttg tgttgttagt ttacctgttg aatacaaaaa agttgaaggt gacaaacaag
    11461 gattattagt tgaggttact ggactattag gtggacactc tggtatggaa attgttttac
    11521 aaagagcaaa tgctaacaaa gcaatagcta gagtattaag tgtattgaat gttgattatg
    11581 aattagcttc tgttgatggt ggtactaagc acaatgctat acctcgtgaa gctaaatgtg
    11641 taatagctgt taataaagct gatgttgaat ctgctaaaaa acaaataaat gacattttaa
    11701 ctgcatttaa acatgagttt actacttctg accctggcat gacttactca gtagctgaaa
    11761 cttctgtaga caaagttctt actaaagatt gcaaagaaaa agttgttcaa atgtcttgct
    11821 taactcctca tggagttcaa tcagtaagtc ttgatataga aggattagtt gaaagttcta
    11881 ctaacttcgc tataattgaa actaaagaat ctactattga atttttaact tctgttagaa
    11941 gttctgttat gtctattaga gatgaaatcg ctgatagaat aagacttttg gctcaagctt
    12001 taggtgctaa ttacgacctt atagctcaat acccagcttg ggaatttaaa aaaggctcaa
    12061 aattagaaaa aatatgcagt gaaacttatg agaaattaac tggtaaagtt cctactgtta
    12121 tggctctaca tgctggtctt gagtgtggat tattacttga taaacttcct catgctgaag
    12181 ctatctctat aggaccagat atgtttgatg ttcatactcc aaatgaacat gttagtatcc
    12241 cttctgtagc taatgtttgg gattatgtta ttgaaatatt aaaatctatg aaccaatatt
    12301 aaatttaagt tttgattcta agtagatttt aattttaagt aatctatata aaattaactc
    12361 tgtaaattaa tactactgtt agtataatat ttactttcaa tattaactta tagaaataaa
    12421 gcctaatgat agataataac atgtatcatt aggctttatt gtaatttaaa tattattaag
    12481 ctttattctg ttttaaaatt aattcttata ctattttaaa tctatttctt ccttgtctct
    12541 tagaatcata aagagcatta tctgctattt gatacagttc atcatactct tgaccaggct
    12601 ttgctataac tacccccata ctaatagata aactttctcc atttggcaac ttaatgagat
    12661 gaatatgatt gtatacatca ttacatttat taataagttc ttccttattt attatatcat
    12721 tgatataaac actaaattca tctccaccca aacgacctac tatatcattt tttgcaaaac
    12781 tttcatcaag tacctttgca acatcaataa gtactttatc tcctaacaag tgaccataag
    12841 tatcattaat cgacttaaaa taatcaatat ctataatcaa taaagcacca tttttttcag
    12901 ttgataaatt caacaaattt tgtgcagtta attttctaat atatgctgca ttataaatat
    12961 tggttaaact atccctttga gccttatcta acaatctatt cttttcctct ttttcagaat
    13021 caatattaca tattttcccg attacataca ttggtatttt tctatcatta taaattattt
    13081 ttgaagtaac tctaatccaa tgaaaagtat catcaagcat aatagaatac atttcagttg
    13141 ttccattttc ttgtgatttt attacatcaa aaaacttttt attatattct ttccttattt
    13201 tatctctttc attttctata ttgccatttt cactttcctt atcattttta tccaaaccat
    13261 aataagtctc aatacccttt tctggcatag aaattatcaa tttatcttgc ttataatcat
    13321 actcaaaaaa gtattcactt gtcatttcat acaactgagt atgttttttg agttctaaag
    13381 atatcttctc gttcatactt gtacgagtat gcaaaccaaa tgccataaat ccaataatac
    13441 aaattaataa actggctaca ataaaaatag tctcaattgg attctgttgc ataatataaa
    13501 gcaacgagaa gtcttgtcta tatgtagtat tttggtagac aattgattgc attttttctg
    13561 atgaaatact tataatagct ttattaaata tattcaataa ttcttgttta tcttttttaa
    13621 ttataccaaa acaaatactc tttgctttat aagtttgagg aactagacgc acatttttat
    13681 acttaggttg attcatatag tactgcatag aataaccttc gccataagtg taatctgcat
    13741 agcctttatt aacagcttct atacactctt ctaaattatc ataccaaact atattctctt
    13801 caaactgacc ataataatta atacctttgg ctagagctac acgttttcca tctaaactat
    13861 tctcatttaa tttattatta atcatcatag tatattgagt agaaagaaaa ggccgagaca
    13921 taactatatt atgttcacga gagtattcat aatcataaat cattcctgct acaatatcaa
    13981 tttcacgctt tttagtcatg ttttctaaat cttcttgaga ttttacaggc acaagttcaa
    14041 attctaagcc tgttttatca catatatgat ttaacaaatc aattgatata cctcttaatt
    14101 cacctgtatc tttatctata ttttgaaatg gtggtttgtt taaaagtact cctactttta
    14161 atttttctgt ctctttaatg taatcagttt cttcatcaga taaaaacatt tgattgttct
    14221 ctggtgtaaa gtatttttca tataaagtag ttgaaaaata tgggtctttc tgttcaatat
    14281 tattaattgc tgagttaagc ttttgagtta aatttgtact acctttagga gtaatgaaat
    14341 aaaatggttt tgatgaaaat tttgctacag tacgtacacc ttctagtggg ttcatactca
    14401 cattaagaag tacatctgct ttaccactct ttagtgcttc tagttgctca tcatttgttt
    14461 tacaatatac caattcaggt gacataagat tcatcttaca atactcatct aactctttaa
    14521 gcctagtatc agaattttct attacagcaa tacggatttt tttcacatct tgtgaactca
    14581 ttatgttatc aatatctgta ttttcatata atgcttgtag tactgtattt gctactccat
    14641 aattattccc tgcataatta tatatttttt gcattggctc attgtataac atacctccca
    14701 ttatatcaat ttcaccattt tctaacatct tcattaattt tgtaatagat tcatttatat
    14761 ctccaggaac ttgaacaaat tcataattcc atccagtata ctgtgcaatt tctagtaaat
    14821 attcatatgt atacccaaaa tagttacctt gctcatctat ttcagttaaa cctttttgaa
    14881 taggataagc aacacgaatg gtacgattat cattacctat agcgttgata ccaaaagaaa
    14941 atatgcaaga agtgaatata gatgtaatta caataattaa taaaagtaat ttttttaaat
    15001 tcactacaca atcctccata tagttaagat ttacagtata aattactgca taaacttgta
    15061 gacatgtaaa aatagattag tatat
//
LOCUS       DAENCV010000091        15069 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAENCV010000091 DAENCV010000000
VERSION     DAENCV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.092"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(141..677)
                     /locus_tag="KQD82_002757"
     CDS             complement(141..677)
                     /locus_tag="KQD82_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG4381217.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(695..2008)
                     /locus_tag="KQD82_002758"
     CDS             complement(695..2008)
                     /locus_tag="KQD82_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG4381218.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2363..2803
                     /locus_tag="KQD82_002759"
     CDS             2363..2803
                     /locus_tag="KQD82_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG4381219.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2988..3176)
                     /locus_tag="KQD82_002760"
     CDS             complement(2988..3176)
                     /locus_tag="KQD82_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381220.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4197..4592
                     /locus_tag="KQD82_002761"
     CDS             4197..4592
                     /locus_tag="KQD82_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381221.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4635..6734
                     /locus_tag="KQD82_002762"
     CDS             4635..6734
                     /locus_tag="KQD82_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG4381222.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6959..12169)
                     /locus_tag="KQD82_002763"
     CDS             complement(6959..12169)
                     /locus_tag="KQD82_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG4381223.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKRLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDDSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKDGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVNLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12184..14679)
                     /locus_tag="KQD82_002764"
     CDS             complement(12184..14679)
                     /locus_tag="KQD82_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4381224.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLERTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         4937 a         2705 c         1679 g         5748 t
ORIGIN      
        1 taattataaa aataaaaaaa gaataggata attttcctac tcttttttct gtacttacat
       61 ataatattaa aactctttat tttttaatat atattttaaa ttttcttttt taataaatct
      121 ttttctttta ataaatctat ttaattttct tcttctatat cagagccaaa gggaatccat
      181 gcattaccaa actctttatc tttaaataaa taagctaatc cagttatttg cttcaatcta
      241 agaatttcat caacttccat acccaggtgc tttgatatcc attcatcaga ttttccaatt
      301 tcacaaagtt cagttataat tttactcatt aaatccacat tatgagaacc tcttgcacga
      361 ttgtgtcgaa tagtacttgc cattctttca tcaagagatt tatcaattac tgaaacagga
      421 agaagtccat tttctctatc atatatatcc tcattttcaa gcattactct atatctatga
      481 aaaccatcta caataatata cttatccttt tcctcattat aataacagac aattggcatt
      541 gtatacccat cttcttttat acttttatac aacaactgca tttctggtgg ggctactgca
      601 tttggattat agtcgttaga ttctatcttt tcgattggca ctgcgatgat tccatataca
      661 ggacttttat attccatagc atcttaactc ctatctaatg aattttttca tatttcttct
      721 taatatattc cgttctaatt ctctgttctt ttgtaggacc aaatcccata aatctacaca
      781 tatggtcatt ttttaaaata caataacaca ttcttttcca acttggtata tctttggtaa
      841 atttaatatc atccgtatca tcaggtatat catccaaaaa tatgactcta ctctttttat
      901 cttttgtatg atttgaaact ccatttaatt ctattttata accttttctc aacaattcac
      961 gtataactat ttctgacaaa ccaccacctg tattctgcca aaactcaata gatgttgaaa
     1021 attttttaat atagctattc cttatatttt caggtaaggt atcaagcaaa aaatatgtga
     1081 aacttttcca tgtgtgtcca tatggcaatt taacactacg atatcccata gcttttgttt
     1141 tgccataaat agaagtaaaa ttaactcctt gtactctagc aaccaatttc acccatacat
     1201 ctgggtccaa tattctgtat aaatttaaac tctcttttgc tgaatcatta aaaggtgatg
     1261 ccactctcat ttttcttgga gctactccag ctttatagaa taagtcataa attttattat
     1321 agtcatagtc aaatttatag tgtgctaacc atatatcttc tatttcccaa tcatatatag
     1381 gagatgccgt ccagacattt ttaaattcct tagttatcca gcactttcct ttatatccat
     1441 gctgtttatt cacaatagaa caataacgtt gtagagattc tgctgctcgt gttcctaata
     1501 agcaaacaac ttttgcacct tcatgtatat ctctaatcca tctaccaaat tgttttgcca
     1561 aactttcttg tctcatttta tatttataat aagtaaaagg attatttttt tgatttatta
     1621 tgtattcttt gactggcatt tcccttaccc ataactcttc tttctcatca tcccatggat
     1681 accaatacat ttcataattt cctacagcag ttcttgtagc cattggtaaa cacacccaat
     1741 atggttcaat atcttcaata ttatcttcaa atactttttc aacatattca gtagtcaaac
     1801 tatattgagc ctcaaaatct tgatgaaata ctcctatttt ggaacttata ttattctttc
     1861 tcttataatc caaaactaaa tttagcagta aaccactatc ttttcctccg ctaaaagata
     1921 catagatata atcaaattca ttaaaaagat aagaaagtct ttcttgaaaa gcttcaaata
     1981 catcggattc taaatatctc ttatccattt gaattatccc ctttcaatta gaagtttata
     2041 aaaccttaca atgcaatatt attatgcttt tttagcagta atcagcataa attatacatt
     2101 atttaaatac acaaatccaa ctaactacat ataattataa tgtaattata cttttgtatt
     2161 ataaactata ttttatcatt attttacatt tttttccaat atatataaat atcatatttt
     2221 tctattagct aaaactaatt actttatatt tctacgtatt agtataataa tacataaaaa
     2281 cttgaataat gcataaaaac ttgacatact gttgtcataa ttactttgct ataattaaaa
     2341 gtaaaaatgg aggtaaattc taatgtatga acgtatgctt gataaaagca ataaaccctg
     2401 tttgtctcaa ataatagaat atataggtaa agatggatat gcacgattgt gtaagttgga
     2461 aacttacttg aaaactcaat ataatttatc aagagaactt cgatttcctt ttggaaacaa
     2521 ttatggttgg ggttacaaat atagccataa atcttttcat ttattttatg ctttttttga
     2581 aaaaggtgca tttactgttc tgattcagat aggtgacaag caagttccag cagtagaaaa
     2641 aacgttggta gctttgacac caaaagctaa agagctctgg gtcactcgtt atccttgtgg
     2701 caataatgga ggctggatta agtatcgtat tttagaagat gcagacttac ttgatattat
     2761 tcaatatata agagcaaaaa aatctccacc taaacaaaat taacttttat tttttgtgat
     2821 ttaaatatag tgtatataaa ttccattctt ttcatataaa aaaaataata taaaaaatat
     2881 ctattagtat aacttattta gtatgtctta ttctactttt tacagataat taaaaagttt
     2941 tatcataaat aaatcataac tagacatcct agtaaatcaa aattaattta ttctttttta
     3001 ttagaatttt tgataacagt ccctatacat atgccaatta tcattccaaa aattagacca
     3061 taacacattg tgatacctac atttccttta gaaataccta tagcacaacc tatagccatt
     3121 ccaagtatca tgcctataaa catacatata ataactaaat tgttgttttt attcatagtt
     3181 ttctccattc aatatatttt aaattgccta gctcgccttc ttagcttaaa acaaagaaat
     3241 ataaattaac tttctttcat tctaatatct gcttgtataa atgtcaaaca acacatttct
     3301 tgtcataacc tcctcttttt gaagtaagta caatctccca gacgcaataa tatcaatttc
     3361 ctaaaaccat ttaagtaact tttatattta cataatattt acaatcataa actatattgt
     3421 tattcattta tacatcataa acactccctc ctaatcatta ttatacttaa aataatgttc
     3481 atttataata atatatctaa cgcactttaa ttgaaaaaac taaaactttg cctttcattc
     3541 ccatatataa ataagtatac tttttgatgc tctattacaa atcaaccaaa ttaattgata
     3601 taaattatca ttttctgttg aaaataccat tcgcacatgc tatacttaat tgcataaatg
     3661 gtaataaatg tcatatgatt ttacaaatct attaataaca attattactt ttactgtatt
     3721 ttaaattttt tggactaaat atcaagaact ttctaaaata caaagtacaa aaaatttata
     3781 taagttataa atttatatga actagataag ctcaaacaat atgttatgta taaactgtgt
     3841 agtattcggt tataaattta tataaattat ataagctaga taaagtatta cctcaactat
     3901 ataagctaga tatataaatt gcttatataa gctagataag aattatttca actatataaa
     3961 ctacatatat aaattacata cataagctag atatataaac tgcatatata agctatataa
     4021 gaattatttc aaccatataa actacatatg taaaattcat gtaaatttac taatataaat
     4081 caaataaaat tactaaactc tctaaactaa ttttgtaaaa catactctat ttttaacatt
     4141 tgttacctta tacataagta caaacaaaat ctaactaaat gaaaggataa tgtaaaatga
     4201 atattttaaa aatgaaaaaa acagggatat ttttaggagg agtacttttt ggtactgtag
     4261 gtgttaagat actttcaagt gattgtgtta aaaaaacttg cactcgtgca acagcaggag
     4321 cattgaaagt taaagattct gttcttgaaa caactacgaa aattcaagaa aatgtagaag
     4381 atattttagc tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca tctaaggtaa
     4441 acgttgtaca agatactctt aaatgcgaac aagaagttct taaaactgaa actataaaaa
     4501 ctgtagaaga tactaattct aatagcatta aatgtgaaga aatcaaagat aacgtaattg
     4561 aagaaaaaaa tagtacttgt acaacagaat aaatttaatg attattatga ttaataatat
     4621 caggaggaag taaattgaat ttttatataa aacacgaaat aagaggtcgt atacgctttg
     4681 acttacaact tggcaaatta aacaacaagc agtccgatac tctcttatat tatttaactt
     4741 ctataaatgg tattaccaaa gcaaaagttt acgatagaac tggtgatgca gtcatttatt
     4801 acactggtga aagagaatac ataataagag aaatcactaa attcagtttt aacaatagca
     4861 aaatagaaaa cctagttcct gaacatacca gcagggaatt aaacaattat tatagagaca
     4921 aattagcaaa taaactgcta gtcaaaagta tctccaaaat attttttcca aagtccttaa
     4981 gatttttttt aattggactt cattctataa aatattttaa aaaaggtctc tcttccctgc
     5041 taaataaaaa aatagaagtt tctgttttag atgccactgc tataggtatt tctattttta
     5101 gaaaagatat caatacggct ggttcagtta tgtttcttct tggtatcggt gaactattag
     5161 aagaatggac tagaaaaaaa tctatagacg acttggctca aagtatgtca ctaaacattg
     5221 aaaaagtatg gttaagaaaa ggtgacactg aaatcttaat tccaatttca gagataaagg
     5281 aaggagattt agtaagtgta actatgggaa atatgattcc attggatgga gttatagtat
     5341 caggagaaac aatggtcaat caggcatcac ttacaggtga atcccttgca gttaacaaaa
     5401 aagaaggctc ttacatatat gctggaactg taatagagca aggcaatatt gttatgtgcg
     5461 tcaaggaaaa agctggtact actagattcc agaaaatagt aactatgatt gaggaatctg
     5521 aaaaactaaa atcttctgta gagagtaaat ttgaacatct agcagataca ttagttccat
     5581 acagcttttt aggaagtata ttaacctatg caattactag aaaccctata aaatcactct
     5641 ctatattaat ggtagatttc tcttgtgcat taaaactctc tatccctatt tcagtactat
     5701 cagccatgag agaatgtaac aataacaaca ttactgtaaa aggtggaaaa tttcttgaag
     5761 gtgttgcatg tgcagatact attgtatttg ataagacagg cacattaaca aaagctcaac
     5821 caactgtttc agatataatt acattccaaa attataataa ggaggatatg ttaagacttg
     5881 cagcttgctt agaagaacac ttcccacact ccatagcaaa tgcagtagtt tatgaagctg
     5941 aaaaacaagg gcttagtcac aaagaaatgc atacagaagt agaatatatt attgctcatg
     6001 gcatttcaac aaaagttgat gataaaaagg ttgtaatagg tagcaaacat tttgtatttg
     6061 aagatgagaa gtgtactatc ccagacggtg aggaggataa gtacaataat ctatcagatg
     6121 agtattcaca cctatttatg gcaatatcag gtaaattatc agcagttatt tgcataaatg
     6181 accctttacg aaaagaagca aaatatgtaa tttcaaattt aagagaatgc ggaataaaaa
     6241 agattgtgat gatgactgga gatagcgaaa aaacagctaa atctatagca agtaaagttg
     6301 gtgttgatga atattattca gaagtcttac cagaagacaa agccaatttt gttaaaagtg
     6361 aaaaactcaa aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat tctcctgcta
     6421 tttcagaatc agatgtcgga attgcaatga gtgaaggtgc tgaaatagca agagagattt
     6481 cagatattac catatcagca gataatttga ataatttaat cgttttaaag cagattagta
     6541 ataagcttat gaaaagagtt gatttaagtt ctaagtttat tatcggcttt aatttaggtt
     6601 tgattttact tggtgtaggt ggatttgtta gaccaagtac atctgccttt ttacacaacg
     6661 catctactgt cggaataagt ttaaacagta tgacaaattt attggaaaac accaatactt
     6721 ataactacca ttaatctcca ttacataaaa catatgatat aaaaaattta taattcatct
     6781 tttaaaaaga atatgtctta aaattagctt ttttctaatc aaaattatat aaagcatact
     6841 ttacaaattt taaactcaac ttatttctat tttttacaaa aaaggagatg attcaaattt
     6901 tcattttgaa acatcccctt ttgtaaattt ctttaaaatt tatatattaa ttatattttc
     6961 aaccaataat tgtattttca accaatcttt cttcatagca ttttaaatca ttttgcttta
     7021 gataattaaa ttcagactct acataaatac cacagcctac caattcaatt gtcaaattag
     7081 gtatattttc ctttagtata ctagtctgct cataaataat tctttccaat aaattaactt
     7141 gtaatataga aaaatgcttc ataatctccc catcactatc tatatctttc ttgatttcat
     7201 accaactctt tgacaaaatt cccttcaaat caatataccc tagactcctc aatatatcta
     7261 tactatcgct ccatgcatac ttactaggtt tatcaaagta tgattctagt ttcttcttct
     7321 gctcatttgt aagtgaatca taagaaagct tcctattaaa atgtgccttc aaagaaatct
     7381 tagccataga atatggactt ctaccagagt ttaatcctgc atttttgaac ttttcaattc
     7441 ctttatcttc tactgaagac catattgagt ttattgaatc tgaatcattg tatttaatat
     7501 caagaccatg tgtaatattt atatagtttt gtggaaatac atattgataa tagacatctt
     7561 ttttatcatc atatagagag aagtatccac tatcatataa atgaatatgc acctttactt
     7621 ttaaactatc ctctaaaata tttcgtctaa ggttcgggat atttgcatac atggttacat
     7681 attttatggc atcaaagttt ttatttaatg gaacttcaaa actaatttca tctcctgtca
     7741 aaagcttttt cacttcttct tcattacaat catatagaga atatgtattt tcaaatccca
     7801 cacgtgattt cccatctaaa ctgcctgtta atgctacctt caaaatatgg ttatctgttt
     7861 tttgccattg gtaagcatct tggtcaagaa actctctaat ctgtctattt tgctctttcg
     7921 cctgatttat aacctcgttt tcatcttctg taatccctac atggcatcta tttaagatat
     7981 ttactatatt gcttccaaat gggaatgtcc atagttgaaa agctgctgcc tcaacctgac
     8041 acaaatatct aacctgattt aattcaattt gatggataag ttttttctga tattcattca
     8101 tctgattacc actatctgac atttgataaa tcaattctcc cattgcaaga ttatcttttt
     8161 tcttatcaat cttatcagta atgataattt gagtttccat catatcctgt acttttttaa
     8221 atattataga aatctcctct gaattttcca tttttaagct tccaaacttt gtgcttaatt
     8281 cagttaaaac ttccttcatc tttaatttac ttatctcatt ttcaaaaata tcttgatttt
     8341 taactgtact atcgaatttt tctacaaatg gtataacaga ttctttcaaa gttttatctt
     8401 taaattccac catttgagta ttcaattcat ctaatttctt aatattattt tctttatctt
     8461 taattgcctc tttaactacc tctattttac tctcaaaact agattttaca ttaagacaat
     8521 tattaacacc actaatcgca gtatttataa gagaacaagc agaacttcca atactcaatg
     8581 catttttaat tttagatgtc ttttgctgtc tttcaatagc ttgtttactt ttctctagtg
     8641 cagctaactt atctttttgc tgtttattat tgtaatttgc ctccaacata cctatttctt
     8701 ttttctggat ttttgtgtaa atttccttat actcgctttc acttaacccc tcttttgctc
     8761 tttgaatatc gtcagctttg tctgcatagt tatcatcatc tatagactta actataatct
     8821 ccttaatttg gtcaaattta gttcttcttt cttcatcaac acctgccaaa ttataagtca
     8881 ttttatctaa agcatttttt ctatcttcac ttagctgttc tccaagtaca aatctcatac
     8941 catcagaatc caatttaaaa tctgaaaaat caaccatcct gttagtccat ttgtattcct
     9001 cttctttagc tgaaagattt tccttcaaag attttgcact gtcaaatgcc cctagacaag
     9061 cttgaccaac accactaaat gcagtcagta aactcaatgc gctttgtatt ccagaaagaa
     9121 ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt cttttttagt tcctctatgt
     9181 cttcttgttc atcttctttc atctttataa tttcttgaat caacaggttc atttttgcat
     9241 taacagaaag cattttattc tcaagttcat tttttaaaac ctctatttgt tgatttccag
     9301 attcaatttg agttaggata ctttccttat atgtattgaa atatccttct tttaactcat
     9361 ctttctttcc ttgttccaat tctcttaagg tcttatccag ataatctgtc aaagaacgaa
     9421 tatctgtaac acgatttcct ccaatttcac tttcttcaat catctgcaca taaaatgctg
     9481 tcttctgctc aagtgcaaat aaaatcatac tccattcgct aaaattctga ttttttccta
     9541 caaaagattc tattctatca gactgcatca atttatttat aaggtattca tagatttcct
     9601 ttgcctcttt ataaaattct ttctttgtat tcatatcatc cagagaaagt actgctttat
     9661 ttttcttctc aaaactatag tacttctgaa gtatttctct atttaattgc atctctattt
     9721 tctcaactgc ataaaaaatt tcataaatat gtgaaaattc taaaatatca gcttctaaaa
     9781 ttcctactgc acacaacagt ctaagatttc tataaccttt aattccagaa aaaccaaatc
     9841 tctcgaaaaa ttcatagaag aatgtatcta ttgacaaaga atactcctgc tttaacaatt
     9901 ccttttcttt tagtgcatct ccagagtgac cgtcttttcc ctctgctccg gctgaacctg
     9961 aatcttcacg tttgctaatt gtccagtcct tatgttcttc ataaagagta tttaatttct
    10021 ctccatcatc tgctgcttgc ttctgattta aagcatgatt aaacacatat ctatcaaaat
    10081 aatagtagtt tctatcacta tcatccattg gaggagaggt acctatatat cttattgttt
    10141 cttcatatat acctctaatt ccaccaagac ctcctgcacc accagctcct ccagagcctc
    10201 cttttcctgc taaaccatga tttccatctt ttttacttat agcacttttt aataaaggag
    10261 cttttccaag gagataaacc tgtccagact tgcttccaag acctgcattt ccaccattac
    10321 ctcctgtgcc tccagttcca ccttgttttc ctttttctgc ttttaccaca tagtattttg
    10381 tacctttaac attagcaaat tcttttgact cttttttaga acctatttta gcatttgccc
    10441 cagcttctcc agttatccct gtatctcctt tttttccatc ttgtccattt ccaccacgtc
    10501 caccatctac gtaaagttta agttctcctc ttttttctat ttctttaaaa gataatatga
    10561 tatcacctga agtacctgca ttatttccat ttcttccatt cattccgtcg taaatagaac
    10621 tttctccaac caatttttct gcatccttac cagatacgtt gattgaataa gtcccttctg
    10681 gtatatgtaa tttttctgta agtagtacaa tattcttacc tgagaagact tctttctcca
    10741 tattacagtc aattttcaac tcacaatctc catacatata tatagatgac aaaatattaa
    10801 gttgataacc agatattatt ttagtcaaat ccgataaatt cactttaggt ataggaatat
    10861 atatagaaag tacattttct ttctcatatg attttatata tgtacaactt tgaatcttct
    10921 ctatcctata ctctgcatct tcaagccttt tggcttcttc ttccctatat tcttcttctg
    10981 ctatccaata tttgatatct ttcaatgtat cttctaaagt attttttagc aatgatttta
    11041 acaactcact tagaccaaca atatgagtct cactacagta actatttaac tctaaaagaa
    11101 gacgtgtttc ttctaatata ttcactttct gtacgccatc accatatgaa agttcttgta
    11161 caaactccat aagctctttc atattattag tcctactaca taaatctgta tacatcttat
    11221 ctaattgaat ctttttatct tttagactat ttatagaaga tagaatggaa atagaatctt
    11281 ccataccttt tttaacactt tctatataag gaactaaaag tttttctagg tctttatagg
    11341 ttacatcaac agctttttgt atttctattt tacaagaagc agtaagcatc aatcccaaat
    11401 ctgttttttc aaatgtttca ggtagatttt tcatttcatt caacaacttt tctggctgtt
    11461 tatctagtgg attacataat ttaatagata caccatctga taatgctaca tttaataagt
    11521 acactaattc cttacaaccg tcgtaatctt tctcttgact atctaggtct tcttttgctt
    11581 tttccaatat atttcttatt ttttcttctg ttacattacc aattagtgtt ttaggtattt
    11641 tattgataag aaagaagaaa ttttctttat actttgcata atcaccaagc atatctttca
    11701 aatttttgcc tgtttctatc aataatgtat gtctagagtc cagtagggat tgatagtcta
    11761 aaataactac catccctttt acctttgaag atgtggaaat agtttccctt acaagcatag
    11821 caccagcaat catatactct gctccacggt tgtcaaaaaa acctgggcaa tcacataatg
    11881 aaaaatctct atttgcaata tctacaactt ctggacataa agtcttagaa tatacaccat
    11941 ttccaatttc gacagctgaa tcttttgcag ttatatatat ttgtccagtc tcttcatccg
    12001 ttttttcttc catcacacag ccttttaaat aatttataca agtactcttt ccaacgcctg
    12061 aagcacctag gaataacaaa attgactttt cttttaagtc atattttcta agctcgtctc
    12121 ttccttcttc caaaaggttt acgatttcac tataaagttt atttgccata catccatccc
    12181 tttctatatt tcggaacaat agccttgata tttttcttta ataagattct cttctacctt
    12241 tgaaacagct gttgataact tagtatttgg agcattaaat cctccattgt ctcctctatc
    12301 tccatattca gcgtattctc ttgctggtcc tgttttacct tcattccagc ttccaccact
    12361 aaattctata tgatatttaa ttatactatc taacttataa tcccaacttg taaaatatcc
    12421 tgtatagtat acagtcttat ctataccact tggagaaaat gaaagtgtat tatatccgtg
    12481 atagccacca cttcctccat atccacctaa acctggtcta ccatttggac ctgcttctcc
    12541 atctttcact gttatatcac aatgattaat tattctattt ttttgttcct ctgaatctga
    12601 aatgaagtaa atattacctt tcttaccagc atttccacct cttccagcat ttccaccagc
    12661 tccacctttt tcaccttttt tgccacttgt acctttttca aaataccctt taaacatctg
    12721 tccgtggaaa taacttgtat atcctgcatc agctccatta cttccagaag ctcctgtttc
    12781 accatttttc ccatctattc ctttagaacc atttcctcca tttgcaatca gtgttagacg
    12841 ataatttcta atttcttctt taatatataa gtaaatatta cctgcatctc taccatcttc
    12901 accatttttc ccatacatag aattatctac aagttctccg ctctttccac ttacattaat
    12961 ttttacatta ctacctacat caagattgtt agaagcaaga ataatatttt tctctgatgc
    13021 aaagttcatt ccaaggttat catccatata aatagtatct ttagcaaaga acataaatgt
    13081 agaaacattg tcatctactt ttctattttt gaaatattcc aatacactgc taattactaa
    13141 gtttggaatt aatgctctta tttcaatacc tttcttatca ttaatattaa ttgtttttac
    13201 tataaatgga tttaaaatgt atttaccaat aaattctcta attatctcat taccttcttc
    13261 tccatatgca cttaatcttg ttatactgtc ttctaactct tcattttgtg taactgtaac
    13321 atttgtcaaa attgaattaa aatcacttac actatataca taatcctttt tatttgcaac
    13381 ttccattaat ttttctctat ttggtaatgt aaatacctgt ttttgcactt cataagaaac
    13441 taaaactttt tctaaattct ttcttattat aatgaagttt tgttccatta tcttcttact
    13501 tgccttttct ataataaatt ccatatgtac agaaggtaat aaaatttccc cagttccatg
    13561 ttctgtatat ttacttaaag catcaattat atctaagttt ccttttatct tattttgtaa
    13621 tttcatgcaa ctatcttgta agaataagcc actttgtttg gctaatacgt tgataaaact
    13681 ctctatagta agacttgttg tactttccaa ccagttttta aatactttta caacctcatc
    13741 atattttgct atttcttgaa tcattgttat acttccaata atattgataa aatcttctgt
    13801 atacgcctta acataatctt ctaggctaga tacgatattg ttttgaattt cttttatcaa
    13861 cgtatctaaa actaatcttg catctgcact aattggatat tggaatgctg atgtcttatc
    13921 tttaacttca tctaattcta gtacttgctt taataagcct tccctctcat ctgatgcaag
    13981 tggcttacaa agatgaatat tttttccatt atcttttagc aattcttcaa ataaccaagc
    14041 tttttcctct atattactgt ctaaattttc gcataattgt tcatattttc tttttataac
    14101 attatatatt tgtttttctg ttctttctaa gaaacttttt gaaatgaaaa aagttatact
    14161 acccttaaaa ttttctttat gaagacacat atttaactgt tggaacaggt ctagtacttt
    14221 tgtaagtcta gaatctaata tatctgcttc tggtatcaat acaattactg cacatataga
    14281 tgcagaattt ttaaatactt cacctaatga aactgctgca ctaatatcat gggctactcc
    14341 cctattgtcg ctaaaacctg cagtatcaca taattttgca tccattcctt ctattggttt
    14401 tctcatattt acaatgttcg ggtataaagt ttgagaaaca gctcctgaac caatctctgc
    14461 cacagggtct aagcaatcaa tatatgatat accagtatct tcatcttcta cttccatcat
    14521 tacacatcct gcaagatagt taattgcagt acttttgcca catcctgtcc caccaagaat
    14581 tagaattaaa ttttgatttt cagccttatt tattgctact tttccttcat ttaataagag
    14641 tgttatttct tctaaaagtt gattgttatt ttctggcatt tattttcccc cttggttata
    14701 tattttattt aaataaagtt taatccctta agtagattac aaagttttta gactatattt
    14761 aaatttgttt tttatttttt atttttaata aatttgcttt ttcactaaat ttgcattttc
    14821 actcaataat tattcttctt tgtttattaa aataactgtg ttgtataata taatctttac
    14881 caacacagtt attctattta atgcgcttat tttattgtat tttatgtaat acagttgttt
    14941 tttatattat acgtccatta ttttcctatg gttattacat atagattttt aattatattt
    15001 gtaccattaa tttaaacatt cctatactac tgcttaagtt ttacacctat accagcttga
    15061 atattttat
//
LOCUS       DAENCV010000092        14647 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAENCV010000092 DAENCV010000000
VERSION     DAENCV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.093"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(1..642)
                     /locus_tag="KQD82_002765"
     CDS             complement(1..642)
                     /locus_tag="KQD82_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG4381225.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(623..916)
                     /locus_tag="KQD82_002766"
     CDS             complement(623..916)
                     /locus_tag="KQD82_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG4381226.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1309..1359
                     /locus_tag="KQD82_002767"
     CDS             1309..1359
                     /locus_tag="KQD82_002767"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381227.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(1885..2637)
                     /locus_tag="KQD82_002768"
     CDS             complement(1885..2637)
                     /locus_tag="KQD82_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG4381228.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(2696..4351)
                     /locus_tag="KQD82_002769"
     CDS             complement(2696..4351)
                     /locus_tag="KQD82_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381229.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(5624..6703)
                     /locus_tag="KQD82_002770"
     CDS             complement(5624..6703)
                     /locus_tag="KQD82_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381230.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(6787..7443)
                     /locus_tag="KQD82_002771"
     CDS             complement(6787..7443)
                     /locus_tag="KQD82_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381231.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            7765..7971
                     /locus_tag="KQD82_002772"
     CDS             7765..7971
                     /locus_tag="KQD82_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381232.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8124..8552)
                     /locus_tag="KQD82_002773"
     CDS             complement(8124..8552)
                     /locus_tag="KQD82_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG4381233.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9028..9966)
                     /locus_tag="KQD82_002774"
     CDS             complement(9028..9966)
                     /locus_tag="KQD82_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG4381234.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
     gene            complement(10480..11292)
                     /locus_tag="KQD82_002775"
     CDS             complement(10480..11292)
                     /locus_tag="KQD82_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381235.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(11318..11947)
                     /locus_tag="KQD82_002776"
     CDS             complement(11318..11947)
                     /locus_tag="KQD82_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381236.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEYLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            complement(12291..13946)
                     /gene="ilvD"
                     /locus_tag="KQD82_002777"
     CDS             complement(12291..13946)
                     /gene="ilvD"
                     /locus_tag="KQD82_002777"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG4381237.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGIVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIRENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(14287..14586)
                     /locus_tag="KQD82_002778"
     CDS             complement(14287..14586)
                     /locus_tag="KQD82_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381238.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         4804 a         2620 c         1584 g         5639 t
ORIGIN      
        1 ttagttattt ttacctgaaa ttttatacat caaataagaa tatataaatg gtagtattac
       61 aattacaaag attacaaaga tattataaaa aggaatatta ataaatgcat taataattaa
      121 taatattcca ccaataagcc aaatccatcc agctaaatgg tgtgtcttat tccaattatc
      181 ttcatcattc agagtccatg gcaaaattat tcctactgag taatttcgtt tacatttagg
      241 aagatagttt ccaaataaaa taactattat tcctaaaatt aatcctgtat aaaaattaga
      301 ctccaaacta actcctgttg aatataaaac catcattatt tgtataatta ctgaaagtat
      361 agcaactccc cattttgata aattttgtat aacatttgaa aaattagctt tcttaggctc
      421 attctcaagt ctaaaccatg tatacaaatg gatacatagc attaacaatg gtacaccaaa
      481 agctgctata acctttggag cataattatc tggatttcca taattatcaa aatgaattgc
      541 aacttcattt ggtaatctat tataaaaata tattgataca ataaatggta taaaacatat
      601 taaagtagat aaaataaatt gtttatttat cgcttttttc attatctttt tctcccttct
      661 ccataaattg tgaaaaccaa agcataacct cttcgaaaac tgaaatattt atttcgtaga
      721 aaataaagtt cttatacttt gtttcaaata tgagtcctgc cttttttagt tgagatagat
      781 gataagaaat tgtagcattc gtcatattaa atttttcact aatttctcct gctgacattt
      841 tattctccct caacataacc aatatttctc ttcttatagg gtctgaaaga gctttgaatg
      901 tttctggaaa ccccaacacc atcacctcgc tatttagaat ttcatctaaa tagaatataa
      961 cataacatcc attttatttc aatagctatt tagaaatttt tctaaatagt tatttctaaa
     1021 tatttattga aaattttcat attaagattc tcaatttact taagtcgctt ttatttaatc
     1081 ttactttatc taattttttt aacttaacaa aagtgtattg caccataatt gttaaagcaa
     1141 aaacctaatg actgaggtac ttatatatat aacaatactt ttaattttaa attaaaagta
     1201 gctaaaccca atttaaaaag tagttaagtc ctatttaaat agaaaagaat gaaacgagaa
     1261 atatactgta taaaagtaca taaaaacatc tactaattag atacaactat aacagaatat
     1321 atttattatt ataataacaa aagaataaaa aaaaattaat ttagcataac cttgttaaat
     1381 atagatttga atatgaattt aaagttatgt aaaattaaaa atacaggaat gaaaattcca
     1441 ttcctgtata aaatctaact tttgagtctc agtacaatct tattttgaga caatcttttt
     1501 tgttttatat tttcttatat ttaaaatata acgcatatct tatgcaacat attcatattt
     1561 ttctttatat tctttcatcg catcattcca agcgtcttgt actgttacac ctcttttatt
     1621 agcaattttt tgtgccagtt caattatctt catagctctt cttgatatgt tcataaacat
     1681 tcctcctaca aattaccata tatttcatat atataaatca aggttatatt tttatgatat
     1741 ataatccttc tattgtaaga ttacagttta tatgtgtatt atatatgaac tatattagaa
     1801 gataattaga atttttataa agctatatga ttagattaaa tataaaagct ttacaattac
     1861 ttataaattt aaaaagtcta taagctaaac taatgtttga tataccaatt ttacgaccat
     1921 aaataatgcc attactaaaa atattggttt tatcatcttt gaacctttct ttatagcaac
     1981 tttagcacct acataagctc ccattatcat cactaatgca aacactatag caattttata
     2041 atctacttga ccatttatca tgaataataa caatgaagtt atattacttg ttaagtttaa
     2101 tatttttgta tttgcagaag catgtaaaaa atcatatcca tatattttta taaatccaaa
     2161 ggtcaaaaaa gttcctgtac ctggaccaaa aaaaccatca taaaaaccca ttataagagc
     2221 cataagcatc cctagtctta aatttttctt attaacagct tcaaaattat cttcttgacc
     2281 taaattttta gaaataaagg tgtaaatagc cactattaaa atcataacca caaccaaaac
     2341 atttaatatc tcttcactta ttgataatac acatctcaca cctagaacac taccaataat
     2401 agtaaatggt actaattttt ttaaaaggtc gttattaatt tttccagact ttgcatatct
     2461 ataggcacta cttatacatc ctgctgatgc tgcaaattta tttgtaccaa tggcaagatg
     2521 tactggcact ccaattgcca taagaacagg catgcttata agtcctcctc ctcctgctat
     2581 agaatctaca aaagcagcta aaaacccccc tatacataaa aaaataactg aaaacataaa
     2641 attcctccat cattctcttt caatttttct tacaatatat attatataat cttatttaat
     2701 acttattatc cagcatattc tttctatatt catttggagt tatactatta aacttcttaa
     2761 atatttgtga aaaataacta ctgttattaa aacctacttt cttacaaata tcactaatac
     2821 tcatgcttct atctaacaga tattttttag attcttctat cctaagaaga tttatatact
     2881 cagttattga aactttattt ctttcattaa aaatagaact caaataattt ggacttataa
     2941 aaaaaacatc tgatatacta ttcaaattta tattttcttt ataatatttt tttatatatt
     3001 cgtttatatt ttcaattaga tttcctttat cttttctcca aaatgctcta ttcttatcaa
     3061 tacactcttt ggaaaattca aataatacat tccataaatc ttcatcactg tttacaagtg
     3121 atattttttt attatattta tttcttaaat aagatataag ttttaaatcc tctaaatctc
     3181 taagcactgt tctagacaat acaaatatca tttcctgaat agtatctcta acatcattat
     3241 atcttaattt gtttgtatct attagattat aaacaccttc taaattcata tttttaattt
     3301 ctttttcaat ataaagttct aattcaaagg gatattcttt tgaagaatta ttgaacatat
     3361 ctatcggatt aataatatac tcttcttcta ataatctact atttgctttt ttaagtgcct
     3421 cttcaagtct tatattttct tttctactct tcaatatctc ctcatctttt atactaagtt
     3481 gattttcaaa aaaattcctc ttagctattt cctgaataaa ttcagacatc attaataaat
     3541 tttcttttaa tacatataac ctacttttag gatttaatgg aaattcattg taaattttgt
     3601 ttatgtattt tatatcaata ccagaactac ttgataattc agataaattt ttatctatat
     3661 caatagactt atatactata aaattaccac attctatata tccaactata ttttcttcta
     3721 atactatagg tatctctaaa ctggaaacac catactcaca atggtacact ttttcaaagt
     3781 tatcattatt tatgctttta acaagttttt tagtacactg ttttatattt ttacaaatat
     3841 cgcaaaattt attttctttc ttagtctcaa atatttcttt atactttaag tcatatacag
     3901 atattctaag cttcaatata ttagatattt gatttgcaag attttcaatt atgtttacat
     3961 taactaactc ctgtatattg ttttcagaaa atactatagg tatttgtctc aaactcgaaa
     4021 atttatgagg aacataagta attaatatta ctaaatcaat tttaccagtg tttataattt
     4081 catgttctga atatggtggg caataaacta tagatgtttc ctttatattg acctttttac
     4141 catcaattat ttgtattcct tctccctgaa tcacatataa aagctgttct tcacttaaat
     4201 gaaaatgctt ttcttggcag tttcccggaa aaaatcttac aattccagca ctaagtctat
     4261 tgaattgtgt ttttgatggt tcgtgtatcc acataacctc tccccaatca aatttttgta
     4321 cttcattatt tgactcaata aaatccacca taaactcacc tcgtagtttt tgtctaaata
     4381 ttttttcttc ccttcttata ttaaaataat atcatcatca ttaactttca ttactataaa
     4441 atcatcatac tcttttaata ctcctgtaat tttagttgca cttagtttac tacaattcag
     4501 ttttttaata tattgataaa ttctataatt acttttttat tttgtaaaat cagtgttttc
     4561 ccaaaataat tatatctata aatgaaagta tttttactgc ttacatcaat atatgttatc
     4621 ataattttcc tttttattgc atatataaat aactgtatat aacattgtcc aatactatat
     4681 agaaatacat ctaagaaaga atattattaa attgttccaa ttagtattat ttttcttata
     4741 aaaataaaaa aactgttgga ttcttttaga aaaattattt tttacatacc ttttttgctt
     4801 agatagtcta tcttctcagc tattttgtat atacactctt ctagttcatg attttatttt
     4861 aatatcttgc ttatattgct gaaataattg aattctttta tctatcatat tgtctcttat
     4921 atcataaaca tattgtttct tatatcacaa aaatgaaagc attcttatat cttcaatata
     4981 taaacctgct ttttttaatc ttgtaataaa tataggatac tctatattct cttcctagaa
     5041 ataactaata ccacttattt ttctattata tagaaagtaa taaatctttt tcttaataaa
     5101 attcaatagt aagtggaata aaattttata tgaataagaa cattataaaa atactttata
     5161 caatagtcaa taaaagatat tagcttcaaa ttatgttaaa gttgtattat ttaaagtgaa
     5221 aaaacaaata aaaaagaaat aatagtataa gtagaaaaat atacttaaaa caaggcgaag
     5281 cctctcttgt atctaaatca agtagagaat taaatctaaa aaagaaaata aatataaaag
     5341 atgactcctg tacattacaa gaatcatctt atggtttcat aatcactcca tggtttttaa
     5401 ctgtctgata taaaggtctc agttcactca tagcatgacc accctttccg attgctgacc
     5461 ccgtcactta tggacaaaat gtccataagt gatagtcctc tcttaaaacc ctctaaatag
     5521 atatatctca agcacctcat tgaaaccagt tgctggaaac aaactgaagc gttctggaat
     5581 agtaaaagaa attgccacca tatccacgtt actgtcctag ttgttacttt gctaacttcg
     5641 gagcaatttc ctctaacaat acgcagtcaa attctgcctt tttgctaaga aatggcagat
     5701 ggtctgcatc tggtataaaa aaaatctgtt tgtctggcgc cactatttca tcaaaaaatt
     5761 tcttcgctgg aagatagggg ctattatagt cattttctcc catgatgtag tatacaggga
     5821 cctgatagtc aactccataa tttcttatgt cagcttcttc cactaaaaac cgcaatacat
     5881 caccttgatt gtcaatcgca tttggattga caatggccgt attttttaac tctctcaatg
     5941 aataatacgg ggttgccagt atactgatta tattgcccaa atccatattt atagaaagcc
     6001 catacttgct ttggtatgtt cttactgtca taagagctgc caaatagctt ggaccatatt
     6061 ttgctggcgg atatggtgca atagcttcca gcgccttgat atctttttgc ttccctgcag
     6121 ctttcaccat ttcaagtgtc ttttcatagc ccacgcgctc attgtccacc atattaacgg
     6181 tctgcgcaat cccaatgtac gcgctgtaat cctccggata ggtttgcaca gccatagttc
     6241 ccagcactgt tccccagcta tgacctataa ggataatttt ctccttgttg aactcttgct
     6301 tcaaatatgc tgtcacctca tggacgtctt gcagaacacg ctccgcgctc atggtctgca
     6361 aaactgctgc cggatcgttc gcaaagtatg ttttgcctac gtttcgctga tcccagttca
     6421 ccacagtaaa atcattttcc cactcatact ggtacagatg taaaaatggc atcattgaat
     6481 taccaggacc cccatgaacc caaaggatga tggggttgtc tgcgttttgt ccccggatat
     6541 ataacgcctg ctgtatgccg ccgatctcca ccatttccat gcggtcgatt ccaggagctt
     6601 ctattttatg gcttgtacgt ataatcgtgg gggctatcag acgcaaaccg gcaaacgcca
     6661 gaacaaaaac agcaactagt atgcaaagtg tgattaatat cattttaagt accttctttc
     6721 atgaattttt ttagccatag atatctcctc taacattaaa aaaaatatgt tgctatttta
     6781 gttttgttac tctacctgtc ccattggttt cctttcataa aaaatattaa ttgattctat
     6841 aatctcatct tccaggatat caactcctat ttgtctcata agaaactgta agaattgaaa
     6901 ccgtttacga agtgtaggaa agtccccctg aaatacatct gtatcacacc aaaagttaaa
     6961 gagcagcaaa attacctctg cgcattcatc tgggaactct gtgatgatgc ttctatcctc
     7021 tatgccttct cgtaaaacat ttgctaatat tggtgctagt tttttcaaat tatcctgtgt
     7081 aaatgccagt atgatatgcg gactttctat agcgcttgta atcatgttac tagattcttt
     7141 tatcacctca tccgttaaat gccgcctaac taaacttcta agcttctctt ttgctgtaag
     7201 tccatgcatt tcaccgagcc attgatttac tgtctctatt atctgttcaa agcgcctttc
     7261 catgaccaca ttaaaaatat cttcttttga actgaagtga tgaaaaattc cgccctttga
     7321 cattcccgaa gcatcaacaa tattttgcat acttgttttg tcatatcctt tttcagcaaa
     7381 caattttgca gaaatggtaa tgatattttc aatagcttgc tttggattgt tctttctagt
     7441 cattatataa ctccttctta ttaaaaaccg actgttggtt tttattatat aagctattcc
     7501 tacttctgtc aagcaacatt atgtttatgg caaaacaata aggtttggtg gttagacagg
     7561 tattaattag actaatgcga ctttccccta gatggaaccc ttggtaaatt tgcaatcttt
     7621 accggctttg attatatctg taagcaaggc aggtatggcc acacctgccc tgtttgaccc
     7681 tcggatattc attcctatcc ctcctctcct tagccattac accaaaacgc cgctcattgt
     7741 ctccaatggc ggcgttttaa agatatgatt tctcatgctg ctgaaatatg caaaaaatat
     7801 aagatgccct ttattatttt tcctacaata gaaaagaaaa gtattgaaga atgggttaat
     7861 attggagcaa atatgctttg ttttgcacaa gatactacct ttatcactta tgttatcaat
     7921 caattattat ctttttataa tgagattgta ccaattcaaa aacaacatta aattataaat
     7981 ctaattttat attaaaagag actaatattt tatctatctc tattaaggat attttcttat
     8041 gtatcataat attagtctct cttattatta tatactgcta tcatcattag ttgctattta
     8101 tactattttt ataatataca aatttattca gctaacgttt tgcctaattc tatacactca
     8161 tcttttgctt cttcatttgg gtcttccatt gctgtaattc cttctccacc aataattgta
     8221 gcaccagcat tttgcatacg ttcttcccag tctctcatcc attcacaatt tccccatcca
     8281 tatgaaccaa ataatccaat ctgtttacct gataccatag attctaattc tgaaacaaat
     8341 ggctccatat acccctcttc tagttgctct gctcccatag atgggcaacc taatgcaaat
     8401 ttatcttctt cttttaaatc acttggtttc agtaagctca catctaaaac ttctggtgtc
     8461 ttacctttta acttcacacc ttcagccaca aaatttgcca ttttttcagt atttcctgtt
     8521 ccactccaat aaacaatata tattttactc atttttacct cctgtagtat tttttaatta
     8581 atcaatagtt agttatagct aactaacatc taaaaaacaa aagatttctt tatctgaaat
     8641 ctctttattt ttctaaatat agaatatcat agttgatttt gaaattcaat acttattgat
     8701 aatgtttatc atttatattt tatattcatt ttataatatc aaaacactgt atcgtatttc
     8761 taatctttca tagaaaaatt tctaacttat agtacttttg catctcttat aaatctttta
     8821 tatgtataag tacatagaaa aacacacctt ttccagatgt gctatctata atatcgtata
     8881 tttatttttc aattctaact agctaatatg tttataaatt ttatcactta atacctttat
     8941 acattgatgt aaaatcttac acctaagtaa tttttattat ttatctcgac tctatataat
     9001 atagctattt tatcatcaaa tcaaaaatta tcttttgcca ttattcttgt atttataaag
     9061 atttaaatat aagtatccat ttccttgttc cgttctaggt attccaagcg ctctcgtaca
     9121 ttttattaat tgtgcatata gttcagcttc atctaaatct ctttcaaact cctctcttgc
     9181 ccattcttta attagtgcta atgagcctga tacgtatggt gcactcatac tggtaccact
     9241 cataatagca agtttattat ccatataagt cgatataata tttcttcccg gagctaccaa
     9301 atctattgta gtatttgaat tactaaactt ttcaaccaaa tagttttcat ttattgcacc
     9361 tacttctatt acctcagcat aactggctgg ataactatac tcacttgtac tagaatcacc
     9421 atctccatta ttacctgctg cacacactac agaaatattg ttttttactg cttgcatgac
     9481 agcatttttt aacttcttat catctttgtt tcccccaaga gacatagata taatatcaac
     9541 cttgttattt acagcaaagt taatagcatt aattatactt tgatatgttc cagtaccatc
     9601 tttatttaat gcttttgcta ttaataattt acaatctgga gctactccca taacttcatt
     9661 attatagtta gatgcagcta taatacccgc cacatgagtt ccatgaccat taaaatcctc
     9721 atatatattt ttatttccat tactgtcatc actaaaattt gcaccaccaa ttatttttcc
     9781 ttttaaaaga ggatgagata tatcacaacc tgtatctatt ataccaacta caatattttt
     9841 accagtataa ccttcatccc acattcccct agcattcatt tgttttattc catatgggaa
     9901 tttatttttt gcacctctta gattttcatt tatttcatac ggaattagtt tcattctttt
     9961 attcatcata tatcctccta aaaattttat tgcttataaa aatcatgaat tttattttat
    10021 aataggttta taaatcaaat taaaacctat atctacttat ttatatttat gatatatttt
    10081 ttatttttag gagtcatatt ttattgtata taaaataata tttagataac tatattaaaa
    10141 aatacatgtt tgcttatata caaaacttac taaaatgtct ttaatatttt tgtatacaaa
    10201 cattatattt tttatataaa aagctcataa aaattttgat tatattttca taagttaaca
    10261 aatgtgggta taatatgaat ttacaaaatt cctctgtttg tataagtgct ttaccaatac
    10321 aacaatatat aatcacattg aagctatatt tgtatttata gtagataatg tatcaaaaat
    10381 tggttatgaa tagtaaaaat accagttaag catattttca tgtttctatt tttaacatta
    10441 aatttccaat cttcatatgt atatttttaa caaaggtgtt tacttattta aaaactctaa
    10501 aactctttca ttaaaatcct gtgcttcttc atatgtagcg tgtcccaatc cctcatagat
    10561 aaatatttcg ctttttttaa ttttttctgc taaatgaaaa gaagcattat ttccaacaat
    10621 tttatcatta gcacctccta taattaaggt tggacatgtt attttattta attcggaaaa
    10681 agcattatgt tctatgcaag aggtcgcttg aataataaat cgtttaaaat cctttggttt
    10741 acctactttt cctaaaaaag gaataaataa ttggtatttt tttaaatatc tttcagaata
    10801 ggatttcttt gctgtgtcaa tcattaaatc attgtaattc tgcttcttcg ccatatcaat
    10861 ccagctacaa ataacatttt ggatagtatc attttgcttt gaagaagtaa ctgccaacac
    10921 aagtttctca actaactcag gataatcaat tgccagatat tgagctatca ttccaccttg
    10981 agaaacaccc attacttctg ctttcataat tccaagtttt ttcattatat ctgcttggtc
    11041 tttagccata tcccttgtgg aatatttttc tgtaatttga ttttttctac taaatacata
    11101 aaccttataa tcttttgcaa attgcttata tctaaaagca aaagcaatcg cctgtatttt
    11161 tccatgtaaa ggaaataaac catctcctaa tcctggtaaa ataattagtg tcttcttacc
    11221 actaccaaat tcaatataat tcactgtatt acttcctatc atcatttgac agctttttgc
    11281 attataaaac atagtctatt cctcactttt cctattatca ttgtttaaat aaatctttaa
    11341 atttttgaat accgtactca tcaattagag gaatatccat tttttttaac atgaataata
    11401 aaaaatccaa cctattacat acttcactgt attcacgttt aaataataca gggtcaagta
    11461 aaatatcaat actaaaaacg agcatttcag ctaattcctc tggatattca caagaaaatt
    11521 ctttattttc aacaccttgt ttaattatct tagcaatatt gggtgcaact acattgttga
    11581 gattacgaac ttcctctatt aaagcgaatg ggattttttc taaccagcta caatgtacca
    11641 tttttctacg tgtgtaatca ttcgcactat gaacaaccaa ttctattatt ttttcatttg
    11701 ctgtaagggt gtcatcagca acaagatact ctaaaaaatt attcatttgt gagtaaagtt
    11761 cttttgtgac acttctcagt atatcttctt tagttttaaa gtgatgatac attgcacctc
    11821 tagatagacc agataaattg ataatatctt gtgtagatgt tgaatcatat cctttagacg
    11881 aaaatagtga aaatgctgat tggataattt tttctttact atttttactt ttttcttctc
    11941 tattcatatt tccctcctaa aaacagactg tctgtttgtt ttttattata cattttaata
    12001 tttattatgt caagaattaa ctgactttca atttcttcat agttcagatg gaatgaaaag
    12061 cattccacat caaactttta aaaagttgta ttacctcaac aaggtctaaa agatacatct
    12121 ctatgctgta ctcgtattcc ttataataga gatgtattgt tttcaaagat aatcaagatt
    12181 ttgctgtgtt tttcatacaa aataaaaagg atacctcatt agtgatataa cacctaagat
    12241 atccaattaa tattttctta actttttcta atttacataa atatagtcta ctattttaaa
    12301 actgctcctg tatttgctga tgttaccaat ttagcatatc tacttaagta tccatgcttt
    12361 attttcggtt ctaaaggttt aaattttaat tttctatttt ctatttcaac ttcatctact
    12421 tttaactcta attttttgtc tggtatgttt atagaaatta tatctccttc ttcaactagt
    12481 ccgattaaac caccttccat agcttctgga gaaatatggc ctatagatgc tcctctagtt
    12541 gcccctgaaa aacgaccatc agtaagaagt gctacatgct tatcaagtcc cattcctgca
    12601 actgctgatg taggagatag catttctttc attcctggac caccctttgg accttcatat
    12661 cttataacta taacatcacc tttgtttatt tttttaccaa aaatagcatt tacagcttct
    12721 tcttctgaat taaaaactct tgctggtcct tcatgaacta acatttcttc tgcaactgct
    12781 gactttttta caacagctcc atttagagca agatttcctt tcaatattgc aagcccacct
    12841 tggttactat atggattctt taatgtatgt attacttctt cattttctat ttcacaattt
    12901 cttatatttt cccttatagt cttgcctgtt acggttttgc aatctaaatt tattccattt
    12961 atctttgaaa gctcgttcat tatagctggt attcctcctg ccatatgtaa atcttcaata
    13021 tgatgttttc cacttggact taattttgtt aaacaaggag ttttttcact tatttcatca
    13081 aaaaaatcta agttaagctc tattcctgat tcataagcta ttgcaggtaa gtgaagtact
    13141 gtatttgtag aaccagccat tgccatatcc acagctatag catttttaaa agcatctatt
    13201 gttaaaatat ctctaggttt tatgtcgttc ttaagtaact ccataacata catacctgca
    13261 tattttgcta tcctttttct ttctccagaa tgtgacgcag cagtaccatt atatggtatc
    13321 cccatgccta aagcttctgt taaacaattc atgctatttg ctgtgaaaag accagaacat
    13381 gaaccacatc caggacaaga gttcatggct aacgcttcta attcttcttc acttactatt
    13441 ccatctttac atgctccaac accctccatt gcagagttaa aatcatatac ttttccattt
    13501 tttttacctg gaagcatagg ccctccactc acaacaatac ttggtatatt aagtcttgca
    13561 gcagccatta acatacctgg cactatcttg tcacaattag gtataagtac caaagcatca
    13621 aaaccatgtg ccatagccat agcttctatt gaatctgcta ttaattctct tgaagccagg
    13681 gaataattca ttcctacatg ccccatagct ataccatcac atacccctat agcaggaaat
    13741 tctaatggca aaccaccact cattcttact ccattttttg cagcttctgc tatttcatca
    13801 agatgcagat gaccaggaat cacctcattt tgtgcattta ctatacctat taatggtctt
    13861 tcaatttctt ctttagtaag acccattcca tacattaacg ctctctttgg agctccttct
    13921 attccttttt ttatatcact tctcataata ttacctcttt cttacttttt taaattttca
    13981 taatattatt ataatatatt tttctattaa ttaattacga aaattgtaaa aaaatattag
    14041 catatactaa tgaaaattta ttacgatttt ttatcaatat cttacacaat cgtattatat
    14101 aatattatct tttaaatata ttttataaac taatatattt tacaaattgt tgagtaatat
    14161 tttatgatat aaatcagaag aagcacttct ataatagaaa gtgctcaact atgaataaga
    14221 ttaataatat atttgcctta gtaatatatt tgccttagat atatagactt ttataaatat
    14281 taaagattaa tcaaatctga tgtttgaaac tttatcccaa ttaatcttcc ttgaatagtc
    14341 ttctggatgt gataatctaa ctatatgagt tgaattatca tagctgtaat aaattgtagt
    14401 agtaccatca actggattct ttttgtatcc ttgatacaca gcactatttg gtactttctt
    14461 ttcaacaaag ctatatgcag atgaagtatc aatagtttct attttattag gaaatataaa
    14521 catataaaaa gctactacta taaaaataca tatgcatgta atactatacc atttaatcca
    14581 tttcataaaa ttcctcctac tttaccaaaa tatactatta ttatttccat atatttataa
    14641 tatttat
//
LOCUS       DAENCV010000093        14601 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAENCV010000093 DAENCV010000000
VERSION     DAENCV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.094"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(35..907)
                     /locus_tag="KQD82_002779"
     CDS             complement(35..907)
                     /locus_tag="KQD82_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4381239.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            1323..2726
                     /locus_tag="KQD82_002780"
     CDS             1323..2726
                     /locus_tag="KQD82_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381240.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            2753..4297
                     /locus_tag="KQD82_002781"
     CDS             2753..4297
                     /locus_tag="KQD82_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG4381241.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            4328..5719
                     /locus_tag="KQD82_002782"
     CDS             4328..5719
                     /locus_tag="KQD82_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG4381242.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            5912..7408
                     /gene="abfD"
                     /locus_tag="KQD82_002783"
     CDS             5912..7408
                     /gene="abfD"
                     /locus_tag="KQD82_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG4381243.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            7543..7818
                     /locus_tag="KQD82_002784"
     CDS             7543..7818
                     /locus_tag="KQD82_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381244.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            7842..9149
                     /locus_tag="KQD82_002785"
     CDS             7842..9149
                     /locus_tag="KQD82_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG4381245.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            9169..10287
                     /locus_tag="KQD82_002786"
     CDS             9169..10287
                     /locus_tag="KQD82_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG4381246.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            10574..11494
                     /locus_tag="KQD82_002787"
     CDS             10574..11494
                     /locus_tag="KQD82_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG4381247.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGNKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            11609..12766
                     /locus_tag="KQD82_002788"
     CDS             11609..12766
                     /locus_tag="KQD82_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG4381248.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(12851..14350)
                     /locus_tag="KQD82_002789"
     CDS             complement(12851..14350)
                     /locus_tag="KQD82_002789"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG4381249.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5607 a         1752 c         2669 g         4573 t
ORIGIN      
        1 tttaaacaaa atattatatc tgtataatat aaatttagtt tatatgaata gatgccatat
       61 actcaataaa aagtttcata ctatgagata tgtattgatt tcttctccaa agaaccccaa
      121 tatttgcagt aattgcacgt tctaatggta ttgaacagag attttctgta ttttgaaata
      181 tttctttata caatattgtt gaagccaagt cattttctag taaataacgt atggtagaaa
      241 gttgatttga gtgtaaaatt atatttggct tagctttttc aagtcttttt gtaataatct
      301 tatagtgata agaaccttca gataagatta caaaaggtac attttttaac atataattcg
      361 atataatagg atgttttgcc aattcatttt tgggatttgt aactaagtat aattctgttt
      421 tatagattgt ctttgaatct atatcatcat aaatatcact gtcaattata ccaatacaaa
      481 aatcaagtgt agattcttca atcattttag ctccaacaaa tgttggaact tcaaaaattt
      541 taagctctat atctggataa attacattaa atctaggaag tatttttgaa agcaaaaacg
      601 tacctaaaac tgtaggaata cctattttga gagaagcttt tctctttttt cctaaatcaa
      661 gtgcgtcaca ttgaaaatcc tcaaattgtt ttataaaatg ttttgacttg tctaaaaaaa
      721 tttttccttc aggcgttaac tctattttat tacctattct gtaaaaaagt tcaaagccaa
      781 gttctttttc taattcttta attgctaatg ttatggatgg ttgagaaata tgaatacttt
      841 ccgcagcctt agtcatgttt ttatatttac agacagtttg aaaatagtat aaatagttta
      901 tattcatttg gtattagttc ctttcattta catattagtg acaagtatat aattaacaat
      961 agcataagtt ttgtatattg ttgtaaaaaa atagaatagt tttaaactat catagcataa
     1021 gtttttgata ttttcaatct attaagaata gtgctattat taaattaaca aaagagaata
     1081 gctggtatgg tttagagtaa atcaatctat attacagtta tatcaacgaa tatcaaaaaa
     1141 ttatctatta aaaaataaaa attattttta caattaaatt gttaattaaa tcataaagaa
     1201 aaaagtgata ttaacaagat aaaatttttt taatcagaaa tgttaattta ataacaaaat
     1261 agagtattgg ggagaaagga gaaatatatt ttttaataat atttgttaat ttaataacaa
     1321 aaatgaaagg gagtgattta gtgaagtttg aattgatgga ttttttaatg aatccttttg
     1381 tccttatgtt tgctgcagta attacaggaa ttttatttgg aaaaattaaa tttgggaagt
     1441 ttaattttgg ggtatcagga gcattattta caggattatt tattggatgg cttgcatata
     1501 gcttggggaa tctaataata gcaaaagggg aaacagcagc aggatataag gcagcaactg
     1561 taatgatggg gaatggaatt atttcttctg acttctttga tttcttttta ataatatttg
     1621 ttgcagcagt tggtctattg gcagcaaaag atatgaaagc tgtattaaag aagtatggag
     1681 caagatttgt aattttagga gttcttataa cttttatagg tggctctatg acttatgcaa
     1741 tgactttact atcaagtgat aaaggaagta gtgcttatga agtttctggc gtttacacag
     1801 gagcattaac aagttcgcct ggattagctg ctgctttaga aacagctggt aaacatgcag
     1861 aagatgtatc aaaagaattt gaaaaagcat caataaaaga taaaaaagaa attttaaaag
     1921 tagtcgaccc agaaggcaaa ttggatgtga atacaactac atcactgact caagagcaaa
     1981 tagataagta tattgcatat gctgaagctg gtgttggtat aggtcatgca gtcgcatatc
     2041 cttttggagt tttaatagta atcttaggtg taaatttcct tccaaaatta tttaggatgg
     2101 acctaaaaga agaaagaaga aaatatgaga aggaaatgaa ggaagcaaga gatagtgttt
     2161 ctggtaaaac gataccagaa gttccattta atataataac atttttcttg acatgtttag
     2221 ctggatactt agttggagga atacatgtat ttatgggacc actagggtac ttcacactag
     2281 gtgcaacagg aggttcacta attgtatctc tagttcttgg atatataggt aagatagggg
     2341 tagtaaattt cagaatggaa gaaaaggttt taaatatttt aaaacaaata gggctagtat
     2401 ttttcttagc aatagttgga ttaagatatg gtggaaaagt agttgattca attatgacat
     2461 ctggtatgca cctagcctta gtagcaatag cagtaggtgt tacagctatg atgataggat
     2521 tcttagttgg caaatacgta tttaaattaa attggatttt attatctgga gctgtctgtg
     2581 gtggtatgac atcaacacca ggtcttggag ctgctgttga tgctcttgat agtgatgacc
     2641 cagcagcagg ttatggcgct acgtatccat ttgcattatt aactaaagtt atattggtta
     2701 tagttttaca caaacttcca atgtaaataa gattttatat ggggggatat ttatgaataa
     2761 tgaaggagca tttgaagaat taagtggaag attaagaaat aaagaattgt gtgatttaat
     2821 aatgaatgca gatgaagctg ctaaaattat agaggatggc atggttattg gagttagtgg
     2881 atttacacct tcaggctatc caaaagcagt accattagca gtttcagaaa gagctaaaag
     2941 tggagaaaag ataaaattaa ccgtatattc aggagcatct ttaggtccag aagttgatgg
     3001 agcatggtca gaagcaggta taattgaaag aagacttcct tatcaaacta attcaatact
     3061 tagaaataat attaataaag gtgttgttga ttatatagat atgcacctaa gtcattctac
     3121 tcaattttta aattatggaa ctatacctaa agtagatgta gctatagtag aagcacttgc
     3181 aataactgaa gaagggaata ttataccaac tagtgggata ggtaattctc catcttttat
     3241 aaaaagtgca gataaagtaa tagtagagat aaatcttgca aaaccaatgg aaatggaagg
     3301 tatggcagat atatatatta cggaaaatcc accaaataga aaaccaatag aaataaatca
     3361 tcctaaggat agaattggaa caacatatat accttgtggt ttggataaaa ttgcaggagt
     3421 agtaattacc aatatgcaag ataagacccg tccattgggg gttgtagatg aagcttcgaa
     3481 aaaaatatca aataatatta ttgctttttt aagagaagaa gtaaaggcag gcagattatc
     3541 aaagaattta cttcctcttc aatctggtgt aggaagtgta gcaaatgcag tattatatgg
     3601 attatgtgaa tctgaatttg aaaatttaac ttgttataca gaggttgtac aggactctat
     3661 gttggattta ataagaatgg gtaaagttac aatggcatct actacatctg taagtccatc
     3721 tccagaagga ttaataaaat ttgaaaaaga tattgatttt tttaaggata agattatatt
     3781 aagacctcaa gaaattagta acaatccaga aatagccaga agaattggtg taattgcaat
     3841 gaacactgca attgaagttg atatttatgg aaatgtaaat tctactcaca taatggggtc
     3901 aaagatgatg aatggaattg gtggttctgg tgattttgct agaaatggag caataactat
     3961 atttagtact gagtcaattg caaaaaatgg tgatatatca tcaatagtac ctatggtatc
     4021 tcacgtagac cacactgaac atgatgtaat ggtaattgta acagaacaag gatatgctga
     4081 tttaagaggt cttgcaccta gagaaagagc tataaaaata atagaaaatt gtgcccatcc
     4141 agattataaa gaacaactta gggattattt aaatagagct tgtaaaagtg gagcaaaaca
     4201 aacacctcac atattggacg aagctttatc atggcatagt aaatttatga gtacaggtac
     4261 aatgaaaaag gctgaaactt ttaaaagtgc attataaaat atttaataaa ttggaggtag
     4321 attttaaatg gaaaaagcag tggaaaactt tgaagattta agtaaggaat acattaatgg
     4381 ttatattgag agagcaagaa aagctcaaag agagtttgaa tgttatactc aagaacaagt
     4441 agataaaata gtaaaaatag ttggaaaagt agtgtattat aatgctgaat atttagctaa
     4501 acttgctgta gaagagactg gaatgggagt atatgaagat aaggtagcta aaaataaaag
     4561 caaagcaaaa gttatatata acaatttaaa agataaaaaa tcagttggta taattgatat
     4621 agatagagaa actggaataa caaaggtagc taaaccagtt ggagtagtag cggcaataac
     4681 tccttgtaca aatccaatcg ttacacctat gagcaatgca atgtttgcac ttaagggaag
     4741 aaatgcaatt attatcacac ctcatcataa agcaattgga tgtagtacaa aaactgtaga
     4801 gatgattaat gaagaattag aaaaaatagg agctccagaa aatttaatac aaatacttga
     4861 ccaacaatca agagaaaata caagaaattt aatttcatca gcagatgtag taattgccac
     4921 tggaggaatg ggaatggtaa aagctgcgta ctcaagtgga aaaccagcac ttggtgtagg
     4981 ggcaggaaat gttcaatgta tcatagatag agatgtagac attaaagaag cagtacctaa
     5041 aatcatagca ggaagaattt ttgataatgg tatcatttgt tcaggtgaac agtcagtaat
     5101 agtagcagaa gaaatgtttg ataaaataat ggatgaattt aaaaataaca aaggttttat
     5161 agttagagat aaagtacaaa aagaagcttt cagaaatgct atgtttgtaa ataaatctat
     5221 gaataaagat gcggtaggtc aatcagttca tacaattgca aaaattgcag gtgttgagat
     5281 accagaagat acaaaaataa tagtaataga agctgatgga ccaggagaag aggatattat
     5341 tgcaaaagaa aaaatgtgtc cagttatatc tgcatacaag tataaaagtt ttgaagaagg
     5401 tgtagctatt gcaaaagcaa atttaaatgt agaaggaaaa ggacatagtg tatctataca
     5461 ttcaaataca gttaaaaata tagaatatgc aggagaaaat atagaagtat caagatttgt
     5521 tattaatcaa tgttgtgcta ctagtgctgg aggaagtttc tttaatggtc ttgcacctac
     5581 taatactcta ggttgtggtt cttggggaaa taacagcatc tctgaaaatc ttgattataa
     5641 gcatctaatt aatatttcta gaattgctta ttatatgcca gaaaatgaag tccctacaga
     5701 tgaagaactt tggggataaa atttagacaa aaaatattca ttctaaatta aagtaggaat
     5761 atattttaat ttagaatgaa tttaagacat aaattaaatt taaattagtt tattggacta
     5821 aatttaaaat attaagagac atatagaaga aggaagtatt ataatttaaa ataaataaaa
     5881 aaatctataa aagagaagga gtggttttat tatggcatta atgacaggag ctcaatatat
     5941 tgaaagtttg agaaaattaa atactaaggt ttatatgttt ggtgaagagg ttaagaattg
     6001 ggttgaccat cctatgatta gaccatctat aaattgtgta gcagcaactt atgacttagc
     6061 tcatgaccca gaatatgcag atttaatgac agttacttca aatatcacag gtgaaaaaat
     6121 aaatagattt ggtcatcttc atcaaagtgt tgatgatttg ataaaaaaag taaaaatgca
     6181 aagactatgt ggtcaaaaaa cagcttcttg tttccaaaga tgtgttggta tggatgcgtt
     6241 taatgcagtg tattcaacaa catttgagtg tgataaagcc catggaacta attatcatga
     6301 taattttgta aaatacttaa cttatataca agaaaacgat ttagtagttg atggagctat
     6361 gactgaccct aagggagata gaagtctatc acctagtgct caacctgacc cagatatgtt
     6421 ccttcatata gtagaaagaa gagaagatgg gataattgta agaggagcaa aggcacatca
     6481 aactggttca ataaattcac atgagcacct tataatgcct actataagta tgactgaagc
     6541 agataaagac tacgctgtat catttgcagt tccttcagat gctgaaggag tgtttatgat
     6601 ttatggaaga caatcttgtg acactagaaa attagaagaa ggtgcagatg ttgatttagg
     6661 aaataaggag tttggaggtc aagaagcttt agtagtattt gataatgtat ttgttccaaa
     6721 tgatagaatt ttcttatgtg gagaatggga tttctctggt atgttagtag agagatttgc
     6781 aggataccat agacaaagtt atggtggatg taaagtagga gtaggtgatg taataatagg
     6841 agcagcagct ttagcagctg attataatgg agcaaacaaa gcttcacata taaaagataa
     6901 actaatagaa atgactcacc ttaatgaaag cttatattgc tgtggtatag catgttcatc
     6961 agaaggtcac aaaacagaag ctggaaacta tcaaatagat ttacttcttg caaatgtgtg
     7021 taaacaaaat gtaactagat ttccttatga aatagtaaga cttgcagaag atatagctgg
     7081 agggcttatg gttactatgc cttcagaaaa agactttaaa tcagacctta aagttggaac
     7141 aagtggaatg actattggcg aagtatgtaa caagtatttt aaagcttctt cagtggcttc
     7201 aacagaagaa agaatgagaa tattaagatt cttagaaaat atatgccttg gttcatctgc
     7261 tgttggttat agaactgagt caatgcatgg ggcaggttca ccacaagctc aaagaataat
     7321 gatttcaaga caaggaaaca taaatcaaaa gaaagaatta gctaagaaaa ttgcgggaat
     7381 aaaaaaagag gaagctttaa atttataaaa agttgatata taaagggaaa ttatatatta
     7441 atgtgtgtta attaatgaaa aaatatgtag tataagagat agttataaaa ttatggtggt
     7501 ttttagctaa gtaaagaaat ttttaataag gagaatttac acatgacctg tggagttaga
     7561 ttttgtgggg gctgtaaccc tagatacgaa agagggaagg ctcttacaat aatcaaagag
     7621 cacttcaatg gtagggtaga ttttagtata gcacaagaga acgtcgagta tgatttgtta
     7681 cttataattg gaggatgcgc aactctatgc gcatcctatc aacaatataa agtgaaaaat
     7741 gaatgtgtta agatgtggga ttttagccat atagaaagag ttataaatgt gattgaatca
     7801 gcaatttgtg aaacttaaaa aagctaaatg gggggatttc attgagttgg caagaattgt
     7861 atcaaagtaa attatgtagt gctacagaag ctgtaaaaca gattaaaaac ggtgatactg
     7921 tagtatttgc acattgtgta ggtgaacctc ctgcactagt agaagctatg attgaaaatg
     7981 ctgaacaata taaagatgtt gagattaaac atatggtaag cttgggtagt ggtggatata
     8041 ctgctaaggg aatggaagct cattttagag taaatccaat gtttgtaagt ggaaatgtaa
     8101 gaaaggcaat tgaaaatgga gatggtgatt ttacacctgc attcttccat gaagtaccaa
     8161 agttgcttag agaaaaaaga ttaaaatgtg atgttgtttt agcacaggta actccaccag
     8221 atgaacatgg gtattgtagc ttaggaacat cagttgatta tacttatgaa gccattaaaa
     8281 ctgctagaac agtaattgtt caagtgaatg accagtttcc tagaacctat ggtgaggtag
     8341 tccatgtatc agagtttgac tatatagtgg aaaaatcaca acctttattt gaactacaac
     8401 ctgcaaagat tggagaagta gaagaagcaa ttggtaaaaa ttgtgcttca ttaattgaag
     8461 atggctctac tttacagcta gggattggtg ggataccaga tgcagttatg ttatttttaa
     8521 ctgataaaaa ggatttaggg attcattcag aaatgatttc agatgggact ttggcccttt
     8581 atgaaaaagg tgttataaat ggtaaatata aaaattttga taaagaaaaa atgacagtta
     8641 ccttcttaat gggtactaaa aaattatatg actttgcaaa taataaccct gcagtagagg
     8701 taaaaccagt agactatgtg aatcatccag caattataat gaaacaacat aagatggttt
     8761 ctataaattc tgctatacaa gttgatttaa tgggacaggt agttgcagag gctatgggac
     8821 ttagacaatt tagtggtgtt ggaggtcaag ttgactttat acgtggagtg tctatgggtg
     8881 aagatggaaa ggcaattata gctatgcctt caataactac aaaaaaagat ggtacagtaa
     8941 tttcaaaaat cgtatctatt gtggatgaag gtgcacctat aacgacatca agaaatgatg
     9001 ttgactatat tgtaacagaa tacggtatag cagaattaaa aggaaaatct cttagagaaa
     9061 gagcaagaaa tttaataaat atagctcatc caagtgtaag agaaagtcta gcagtagaat
     9121 ttgaaaagag atttaaggag aaatattaat aatagagggg tgttaataat ggaagcactt
     9181 agagtagttc caaagatatt ttactttgat acatttaaag aatttaatga agaatttaaa
     9241 ataggaaaaa atgatttagt aattactaat gagtttatat atgagccata tatgaaacct
     9301 cttggaatag atacaaattt aatttttcaa gaaaagtttg gaacaggtga accatctgat
     9361 gaaatgatag actccatgac aaaggaaatg aagaaataca attttgatag aattattgca
     9421 tttggaggag gaacaatagt tgatatatgt aagatattag cacttgatgt tccagaaaag
     9481 tctattgatt tatttgaagg agaagtatca cctaaaaaag taaaagagtt agtagtagtt
     9541 cctacaacat gtggtacagg tagtgaagta acaaatgtag ctattgcaga gttaaaatct
     9601 aaacatacta aaaaggggct tgcagtagaa gaaacatatg cagactatgc agtgttaatt
     9661 ccagagacag taaaagggtt accttacaaa ttctttgtta caagttcggt agatgctctt
     9721 atacatgcta ttgagtctta tctttctcca aaagcaagtc catttacaga gatgtattct
     9781 ctacaagcaa taaaaatgat tatggatggg tataagaaga tagttgataa aggagaagaa
     9841 gagagattta atcatcttag agactttgta cttgcatcaa attatgcagg aattgctttt
     9901 ggaaatgctg gttgtgcagc agttcatgca ctatcttatt caataggtgg agctttccat
     9961 gttgcacatg gtgaagcaaa ttatcagttc tttactgaag tatttaagat gtattctaga
    10021 aaaaaaccta atggaaaaat tataaaatgt acaaaaattt tagcagatgc ccttgaatgt
    10081 gaccctaact gtgatgttta tggagaatta gaaaagtttt taaataaatt aatagctaaa
    10141 aaagctttga gagaatatgg aatggttgaa agtcagatag atgaatttac agatagtaca
    10201 atagcaaatc aacaacgact acttgctaat aattatgtag aacttagtag agaggaaata
    10261 agagagatat ttgcaaatct ttattaatac aattttttat ttatatgtat agtcaacata
    10321 aacaatgcta attatattta tgtacaaata aaaataaggg aacttaaatt ttaattaaag
    10381 ttaagttctc ttatttttat atggagttaa atataagtat atttttaatt taaaatatgc
    10441 ttatatttaa gtctattact ttatttgaaa caatttgtag aatttcaaat tccttgacag
    10501 taataagtaa gtaattgtat aatcaaactg caatatatta ataatattag tgaaaatgaa
    10561 aggattataa aatatgtata aatcagaaaa tttttcaaaa aaatctgtat taataaattc
    10621 atttttaagt aaatttttaa aaataattgg atggggtttg gtaagcttat ttacattatc
    10681 agccttagta ctttcaacta tttccattat aattggatgg gctgtaatac ttcctattca
    10741 agtatgtgca gtatttttac ttataggagg agtttgtatt tttaaaggta agagattagg
    10801 agaccaaatt tctcgttata aaaaatattg tacaattata aacaatagaa atattattcc
    10861 agtagaactt attgcagaaa acacatctaa atcattaaat tttataataa aagatgtaca
    10921 aaagatgata gacaaaaact actttataaa tacttatatt gataaaagaa ataatcagat
    10981 tgtgttaaca aatgaagatt ttattccacc aagatatgaa gatgtagcat atgaagtcaa
    11041 agaagaagaa aagactgatg aatccaatga agttgataca attataaata aaggaatgaa
    11101 ttatctaaca caaataaaag aagctaataa aaatattaaa tcagaatcaa tgggcaataa
    11161 aattgtacaa gtagaagatg ttacaagtaa aatatttgat gttgtaaaac atgacccatc
    11221 aaaacttact caaatacaaa aatttatgga ttattatctt ccaaccactt taaaactatt
    11281 aaattcatat catacattgg aagaacaagg aattgataga gaaaatataa caacaactat
    11341 ggaaagtatt gaaaatacaa tgaatacgat tgtagtagct tttgaaaatc aattggacta
    11401 tctgtttgaa gatgaagcca ttgatatttc tacagatatt actgttttag agaatatgtt
    11461 agtccaagaa ggattaacga gtggtaaatt ttaacttcaa tagaaaaaat attctgttat
    11521 ttttaaacaa ttaattagtc ataaagtata aaatgtgtat tatataagtt aatcagtact
    11581 ttatgaagga agaggagaga aagatacaat gaataattta gatgatatac cagttatgcc
    11641 aactttgaca cttgacccat ttggagaaga atcaaacact aatgatatag ataattctga
    11701 cttattaatg aaaaaggatg aaaaagaccc agaagaagaa aaactgtctg aatctgaacg
    11761 caagatggta aaggaatttg cggaaaaaat agatattaca aatacaaata tgattttgca
    11821 atatggagct ggtgctcaaa aaaaggtagc aagtttttct gaaacagctt taaagagtgt
    11881 aaaaacaaga gatttaggag aagttggaga tatgcttaca aatctagtta ctgatttaaa
    11941 agcattttca gcagatgaaa aagaacaaag tggtttttta ggaattttta aaaaggcaaa
    12001 taataaaatt tcaaatttaa aaactaagta tgatagtgca gaggtaaatg tggataaagt
    12061 aagcaaagag cttcaaaagc atcaggttaa aatattgaaa gatatagcaa tgcttgataa
    12121 gatgtatgaa ttgaacttag cttactcaaa agagcttact atgtatatta tagcaggaaa
    12181 acaaaaatta aaggatatga aagaatatga gatgccaaag ttgagagaaa aagctcgttt
    12241 atctggttca acagaagatg ctcaatctgt aaatgatatg gtttcacttt gtgacagatt
    12301 tgaaaagaaa attcatgact tagaattaac tagaatggta tcgcttcaaa tggctcctca
    12361 gataagattg gttcaaaata gtaataatct gatggcagaa aagattcaat caactatagt
    12421 aaataccatt ccattatgga aaaatcaaat agtgcttgct cttggaattt ctcacttaaa
    12481 tcaagctatg aaagcacagc gtgaagttag taatatgaca aatgagcttt tattaaagaa
    12541 tgcagaaact ttgaagatgg gaacaataga aacagcaaaa gaatcagaaa gaggaattgt
    12601 agatatagac acaattaaga aaacgaatca atctctaata tctactattg atgaggtagt
    12661 taaaatacaa catgatggta gaataaagag acaagaagca gaagttgaat tatcaaaaat
    12721 agaaaatgaa cttaaaagta agcttcttga atttacagtt aaataaaaat tatactaata
    12781 taaaaagaga ctgtctcaaa atagagataa gtctcttttt cattgtttaa atgattaaag
    12841 tatatttata ttataaattt ctattatctt ttaacataac atcatgagct ccaccttcaa
    12901 ctatactagt agaagataca agtgtaagtt tagcattttt ttgaagttca ggaatgctaa
    12961 gtgctccaca gttacacata gtagaacgaa ctttacttaa agaaagagtt acattgtctt
    13021 tcaaagaacc agcataaggc acataagagt caacaccttc ttcaaaagaa agtttcttat
    13081 ctccaccaag gtcatatctt tgccagtttc tagcacgagc agaaccttct ccccaatact
    13141 ctttcatata actaccattt atattaactt tgttagttgg actctcatca aaacgagaga
    13201 agtatctacc aagcattata aaatctgcac ccatagctaa agctaaagtt atatgatggt
    13261 cataaacgat accaccatca gagcaaattg gaatataaat acctgttttt tcaaagtatt
    13321 cgtctctagc ttgagcaact tcaattatag aagtagcttg tccacgtcct ataccttttt
    13381 gttcacgagt tatacatata gaaccaccac caataccaac tttaacgaag tcagcaccag
    13441 cttcagctag gtaaaggaat ccttcgcggt caactacatt tccagctcct accttaactg
    13501 tatcaccata tttttcacga atgaaatcaa gtgttatttt ttgccactca gaaaaacctt
    13561 ctgaagaatc tatacataaa acatcagcac cagcttcaac taaagcagga acacgttcag
    13621 caaagtctct agtattaata ccagctccaa ctacatatct cttagaagaa tctaaaagtt
    13681 caagtggatt ttctttatga gaagagtaat ctttacggaa taccatatac ataagattac
    13741 cattatcatc aagtacagga agagaattaa gtttgtggtc ccatataatg ttgtttgctt
    13801 cctttaaagt aacatctttg ttagcataaa ctatagatga cataggagtc ataaattcac
    13861 taactttagt atctaaatcc atacgactta tacgataatc acgacttgca acaataccca
    13921 ataatttacc agttgaagtt ccatcttcag taacagctac tgtagaatgt ccagtttttt
    13981 cttttaagtc aagtatgtct tttaatgtgt tatcaggttt aatatttgaa tcacttacaa
    14041 caaaaccagc tttgtgggat ttaacacgtg ctaccatagc agcttcattt tcaatagttt
    14101 gagaaccgta tataaatgaa ataccacctt ctttagcaag tgcaatagcc attttgtcat
    14161 cagaaacaga ttgcataata gcagaagtca aaggtatatt catataaata tctgcttcct
    14221 cgcccttttt aaatttagta acaggtgttt ttaaacttac attagcaggt atacaatcag
    14281 tagaagagaa acctggtaca agaaggtatt cactgaaagt atgtgatggt gtatcaaaat
    14341 aaaacgccat tttttaatca ctccttatat ttttatactt tttataattg atagtattaa
    14401 ttataactta ttctttaatt ttattaaagt attttgtcaa atattgagat ttttgaaaaa
    14461 tttaaactat tttaattaaa tttgtttaat atatattgcc ttatagggta aagttgtata
    14521 ttaaataatg tttagtgttt aatagtagct aatttaaaat taatctttag tgattcatat
    14581 aattacattt gtatagttta t
//
LOCUS       DAENCV010000094        14105 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAENCV010000094 DAENCV010000000
VERSION     DAENCV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.095"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            44..1489
                     /locus_tag="KQD82_002790"
     CDS             44..1489
                     /locus_tag="KQD82_002790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381250.1"
                     /translation="MKEELNIVPFLAESLSINKKCYKLIDKYYNENKLKYSSLAKDNI
                     FYNSRIASEGSIIQEYYFKKSLGILLERDEKKITKIFKVGYTIAYNYIISIQTFKAST
                     FLRKLMLKNKIESFSEDELNGNMLVAISLCGALGKEVDESDEIYIKVIRNLILRNENY
                     KHEKVLSIDRLSKDEQKLISKIELRLKSTYLKEYIPSSYTIGIDGSIGTFNLDALTDL
                     DRNLLGFDYIYDLEELSLISIVGRDILKSKQIQELILCYCNLQKNIEDENSINYEDLF
                     KFIIPAIDIRYLAREYKKAKQFFFNNFDEELKESIEEKDLKLDSIKKENSLLEAENEK
                     LKSELELLQKDKLRLENEIKAQLTSKEELVQLRNFMFNQQQEQEEKVLIDESINLKNI
                     KAIIFGGHPNWILKMKEKLTNFEFISADTINFNVNILNSYEYVFINTNFIGHAMYYKI
                     IENLNEDNKLRYINNINVDRAIEAIKNAIKI"
     gene            1500..1868
                     /locus_tag="KQD82_002791"
     CDS             1500..1868
                     /locus_tag="KQD82_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381251.1"
                     /translation="MIDIDINKRIKEIREDINLSQQKFGEKLGVSRDVISNIENNRVE
                     VKTVFITHMCEVFSVNQVWLETGEGRKFIEQECDIVLGEVFANITVSKNENLNELIIN
                     ISKLNDTYINNLNEIVKGLI"
     gene            1888..2268
                     /locus_tag="KQD82_002792"
     CDS             1888..2268
                     /locus_tag="KQD82_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381252.1"
                     /translation="MSDETISKRIRKIRKNANLSQPDFGGKLGVSKDVISNIEYDRVE
                     PKPLLINHLCSVFNVNKEWLINGTGDMYVSVKEDISSILGEVFYKIASSKDENLKELI
                     INISKLDATYIASLNEIVKRSIQR"
     gene            2274..2621
                     /locus_tag="KQD82_002793"
     CDS             2274..2621
                     /locus_tag="KQD82_002793"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381253.1"
                     /translation="MCDIGKRIKILIENEKIKQVEFANKIKVDPSYISKFLSDKSKVI
                     PSDRLIDDICSTFNVNKEWLKNGDGEMYIENKCNRNISEATDLLMELSIEFQECAINQ
                     IKDLIKLQHSLKK"
     gene            2708..3040
                     /locus_tag="KQD82_002794"
     CDS             2708..3040
                     /locus_tag="KQD82_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381254.1"
                     /translation="MKKYFKEEWNYIADAGYEIDNIESTKIMVNNFEVSIKMSEDFKH
                     KGDIEYTFNIPYDHEIFTKEYNEVLNLEQPCDCDQFCDELSDLRARIEEGIEKYLKDN
                     DKLKEIEY"
     gene            3185..3661
                     /locus_tag="KQD82_002795"
     CDS             3185..3661
                     /locus_tag="KQD82_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381255.1"
                     /translation="MDKKEAKKFLKVVDMNIDKIKEEATKIYKECYLIETTDTIRISI
                     NLKGVVKSTISSWQGYSDDIFNMREIIVYEFSQGEVQIEDLLGELCFLNDYEEFATWC
                     DTESETLNWNSYEKFNKENFDELVERNIEDSLPDFLEELSESIENCKQELKSMIEI"
     gene            complement(3967..4266)
                     /locus_tag="KQD82_002796"
     CDS             complement(3967..4266)
                     /locus_tag="KQD82_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381256.1"
                     /translation="MDVEQSKKVYLSIGKELSKKMTKSELTKFSQEVANYLQYAGLDK
                     NMKIKLFQTILEMVARKKITLSSDSEFTEGILQSDEAEMTTNIGFIIEGLSYHND"
     gene            4652..5053
                     /gene="tnpA"
                     /locus_tag="KQD82_002797"
     CDS             4652..5053
                     /gene="tnpA"
                     /locus_tag="KQD82_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG4381257.1"
                     /translation="MYKSNENIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIDNQPIRSKKK"
     gene            5050..6213
                     /locus_tag="KQD82_002798"
     CDS             5050..6213
                     /locus_tag="KQD82_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4381258.1"
                     /translation="MIKTHRVKLNLTRTQFELIREKQMESANCWNHIVNLSKEYYFEH
                     KQWIKKNDIQKSIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYKTHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            6500..7072
                     /locus_tag="KQD82_002799"
     CDS             6500..7072
                     /locus_tag="KQD82_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG4381259.1"
                     /translation="MTWNDLKNNDITYIVEYINSKLNDSKSLTKVAIELGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNRFNMTIEDDNNHNIVIDNQFKN
                     NIISLAKDYDKIQDVLNWFENKEDTNVIEVVQDGIKIDLPSKDAIRTTVRLNKDAWNL
                     FDEFCEKFREFNKSDLMSMALLEYIKKYDK"
     gene            7149..7322
                     /locus_tag="KQD82_002800"
     CDS             7149..7322
                     /locus_tag="KQD82_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381260.1"
                     /translation="MVDKITIDEVLEFIKSKGYIYSQEIRKELDNLCPVSDNEIDKCI
                     EENDDVFRRLASK"
     gene            7319..7687
                     /locus_tag="KQD82_002801"
     CDS             7319..7687
                     /locus_tag="KQD82_002801"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBG4381261.1"
                     /translation="MITLEYICKINKKMIERYGGAGIGIRNKNLAISIIDGINQEVFG
                     RVMYPTVEEKISHIVVGIIQGHIFIDANKRTGLATLLILCDKYNIDVNYDKENLAIEI
                     ALNKINESDLSNMLKKWNKK"
     gene            7884..8810
                     /locus_tag="KQD82_002802"
     CDS             7884..8810
                     /locus_tag="KQD82_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381262.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEGNDYIVEIVGNDKEKIVKDMNQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLTQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRSIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQGSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMQLNEKKYTQSGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            8815..10383
                     /locus_tag="KQD82_002803"
     CDS             8815..10383
                     /locus_tag="KQD82_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381263.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSNYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            10857..11123
                     /locus_tag="KQD82_002804"
     CDS             10857..11123
                     /locus_tag="KQD82_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HBG4381264.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNINYIDLKDKQYYVVTQGRR
                     SKRFNNEDVSEIKKDLDNGMSLRKCAEKWNCSTRTIQDIKQNKY"
     gene            11192..11437
                     /locus_tag="KQD82_002805"
     CDS             11192..11437
                     /locus_tag="KQD82_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381265.1"
                     /translation="MKWSEVRNLYPNSFVKLEILESHIQNEKEYIDEVALIKVIPNNK
                     EALKEFNRCKDKEIVYNTKNKEFVIDIIKHIGIRRGL"
     gene            11439..11825
                     /locus_tag="KQD82_002806"
     CDS             11439..11825
                     /locus_tag="KQD82_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like domain-containing protein"
                     /protein_id="HBG4381266.1"
                     /translation="MKNFKYKNGLLYTDIEILIENKNILVKDTIIDTGASHTIISSSY
                     LEESDIGFNDDDTIVKASGYGGTVQYSVRKLVNKVACGDITLENIKLDFGEIDPEEKV
                     NGLLGLDFLRNANLIIDLVDNLLIKK"
     gene            11855..12118
                     /locus_tag="KQD82_002807"
     CDS             11855..12118
                     /locus_tag="KQD82_002807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HBG4381267.1"
                     /translation="MSIKYKDKVVFIVDSSEKEKLDKSGIAYETLENENYYVVQQGRR
                     SKRFNDEMQNQIRKELENSTIRAIAKKYKTSTGIIQSIKDFKY"
     gene            12205..12468
                     /locus_tag="KQD82_002808"
     CDS             12205..12468
                     /locus_tag="KQD82_002808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381268.1"
                     /translation="MSIKYNNEKVFLVSENDKEKLEKAGINFVSLDGNYYVVSQGKKP
                     KKFTDNEIREIKNDLDNGLSIRKCAERWDCSTNVIMKIKQNTY"
     gene            12542..12718
                     /locus_tag="KQD82_002809"
     CDS             12542..12718
                     /locus_tag="KQD82_002809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381269.1"
                     /translation="MLENLIKEYFLDSFCIENINTDMKNLEIKIDNIYNCLNNTLNEN
                     QRKLLSQFDELLKI"
     gene            12735..13913
                     /locus_tag="KQD82_002810"
     CDS             12735..13913
                     /locus_tag="KQD82_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381270.1"
                     /translation="MQELKPVARISKSKLKDILDIFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSNKLNEFCSNCPKLKTISEFSYVAEKETELGMIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSVVTARHNHNVLLQVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRIEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
BASE COUNT         6004 a         1304 c         2235 g         4562 t
ORIGIN      
        1 aatttaatat cttttaattt taattgataa gcgaggtttt ttaatgaaag aagaattaaa
       61 tatagtacca tttttagctg agtctttgtc gataaataaa aaatgctata aactcataga
      121 taaatattat aatgaaaata aattaaaata ttcaagtttg gccaaagata atatttttta
      181 taattcaagg atagcaagtg aaggaagtat aattcaagaa tattatttta aaaaatcttt
      241 aggtattcta ttagaaagag atgaaaagaa aataacaaaa atatttaagg ttggttatac
      301 aattgcttac aattatatta tttctatcca aacatttaaa gcaagtacct ttttaagaaa
      361 acttatgtta aaaaataaaa ttgaaagttt ttcagaggat gaattaaatg gtaatatgtt
      421 agttgcaatt tctttatgtg gagctttagg gaaagaagtt gacgaatcag atgaaatata
      481 tataaaagta attagaaatt tgattctaag aaatgaaaat tataaacatg aaaaagtttt
      541 atctatagat agactaagta aagatgaaca aaaactcatt agtaagatag aattaagatt
      601 aaaatctaca tatttaaaag aatatattcc aagctcgtat acaattggaa ttgatgggag
      661 tataggaact tttaatttag atgctttaac agatttagac agaaaccttt taggatttga
      721 ttatatatat gacctggaag aattaagttt aatttcaatt gtaggaagag acatacttaa
      781 atctaaacaa atacaagaat taattttatg ttactgtaat ttacaaaaaa atatagaaga
      841 tgaaaatagc attaattatg aggatttatt taaatttata attcctgcta tagacataag
      901 atatttggca agagaatata aaaaagctaa acaatttttc tttaataatt ttgatgaaga
      961 attaaaagaa tctatagaag aaaaagacct taaattggat agtattaaaa aagaaaattc
     1021 attattggag gcagaaaatg aaaagttaaa atcagaacta gaattgttgc aaaaagataa
     1081 acttagatta gaaaatgaaa tcaaagcaca acttacatct aaagaagaat tggttcaatt
     1141 aagaaatttt atgttcaacc agcaacaaga gcaagaagag aaagttttaa ttgatgaaag
     1201 tataaattta aaaaatataa aagcaataat atttggtgga catccaaatt ggattttaaa
     1261 aatgaaagaa aaattaacta actttgaatt tatatcagca gatacaatta attttaatgt
     1321 aaacatattg aatagttatg agtatgtatt tataaacact aattttatag gacatgctat
     1381 gtactacaaa attatagaaa atttgaacga agataacaaa cttagatata taaataatat
     1441 aaatgtagat agagcaatag aagctattaa aaatgctatt aaaatttaag gagcgtatta
     1501 tgattgatat agatataaat aaaagaataa aagaaataag ggaagatata aatttatcac
     1561 aacaaaaatt cggagaaaag cttggtgtga gtagagatgt tataagtaat atagaaaata
     1621 atagagtaga agttaaaaca gtatttatta ctcatatgtg tgaggttttt agtgttaatc
     1681 aagtgtggtt agaaactggt gaggggagga agtttattga acaagaatgt gatattgttt
     1741 taggagaagt ttttgctaat ataactgtat ctaaaaatga aaatttaaat gaattgatta
     1801 taaatatttc taaattaaat gatacatata taaataattt aaatgagatt gtaaaaggtc
     1861 taatttaaat agtgggaggt attataattg tctgatgaaa ctataagtaa gagaattaga
     1921 aagataagaa aaaatgctaa tttatctcag ccagatttcg ggggaaaatt aggtgtcagc
     1981 aaagatgtta taagtaatat agagtatgat agggttgaac ccaaaccttt acttattaat
     2041 catttatgta gtgtatttaa tgttaataag gaatggctta taaatggtac aggtgatatg
     2101 tatgtttctg taaaagaaga tatttcttcg attttaggag aagtgttcta taagatagct
     2161 agttcaaagg atgaaaattt aaaagaacta ataattaaca tttctaaatt agatgctaca
     2221 tatatagcgt ctttgaatga gattgtaaaa aggtcaatac agaggtgata ttaatgtgtg
     2281 atataggcaa gagaattaaa atcttaatag aaaatgaaaa aataaaacaa gttgagtttg
     2341 caaacaagat aaaagttgac ccatcttata tatctaagtt tctatcagat aaatctaaag
     2401 taatacctag cgatagatta atagatgata tttgtagtac attcaatgtt aataaagaat
     2461 ggcttaaaaa tggagatgga gaaatgtaca tagagaataa gtgtaatagg aatatttcag
     2521 aagcgactga cttattaatg gaactttcaa ttgaatttca agaatgtgca attaatcaaa
     2581 ttaaagactt aataaaattg caacattcat taaaaaagta ggagagatta atgtctgaaa
     2641 aagatgaaat tataagatta aatttaatag aattaagaaa gattttaaaa taaaaagagt
     2701 gatttatatg aaaaagtatt tcaaagaaga atggaactat attgctgatg caggatatga
     2761 aatagacaac atagaaagta ctaaaataat ggtaaataat tttgaagtaa gcataaaaat
     2821 gagtgaagat tttaaacata aaggtgacat agaatatact tttaatatcc catatgacca
     2881 tgaaattttt acaaaagagt acaacgaagt tttgaattta gaacaacctt gcgattgcga
     2941 ccaattttgc gatgaactat cagaccttag agcaaggatt gaagaaggca tagagaaata
     3001 tttaaaagac aatgacaagt taaaagaaat agaatattag atgtaattga atttatttta
     3061 aaaaattata aaattgaata taaaaatcat aaagctgtat tgttataata cagttttttt
     3121 aatacaaaaa tacagatata tcttgaataa tattttaaaa taggaggaaa ttaatatggg
     3181 taaaatggat aaaaaagaag ctaaaaaatt tttaaaggtt gtagatatga atatagataa
     3241 aataaaggaa gaagctacga aaatttataa agaatgctac ttaatagaaa caactgatac
     3301 tataagaatt tctataaatt tgaaaggagt tgtaaaaagc acaatttcaa gttggcaagg
     3361 ctattcagat gatattttta atatgagaga aattattgtc tatgaattta gtcaaggaga
     3421 agttcaaatt gaagatcttt taggagaact ttgtttttta aatgattacg aagaatttgc
     3481 tacttggtgt gacactgaaa gcgaaacttt gaattggaat agttatgaaa aattcaacaa
     3541 agaaaatttt gacgaattag tcgagagaaa tatagaagat agtttaccag attttttgga
     3601 agaattgagc gaaagtatag aaaattgtaa acaagaatta aaatcaatga ttgaaattta
     3661 aatgaaagag tattattatt aatgtatata gtatctttaa gattaactac gagggattta
     3721 aaatattttt taatttattt agaaatgata ttataataag aattttcaga ttaactacaa
     3781 gggatttaaa tgtcaattta atttatagag agatgatata atggcaattt tttagattaa
     3841 ctataaggga tttaaatatc aatttaatct atatctatat gatataatat cacattattt
     3901 tatattaact atgaggaaat aaaaacagtg cattcagata aatacactgt ttttttaatg
     3961 ctaaaattag tcattatgat aacttaatcc ttcgattata aatcctatat tcgtcgtcat
     4021 ttctgcttca tcactttgta atattccttc tgtaaattcg ctatcgcttg ataatgttat
     4081 ttttttcctt gccaccattt ccaaaattgt ttgaaatagt ttaattttca tattcttatc
     4141 aagtccagca tattgcaaat agtttgctac ttcttgcgaa aatttagtta actctgattt
     4201 agtcattttt ttagataatt cttttccaat gcttagataa acttttttag attgttcaac
     4261 atccattttt ttcaacccct ttttagatta taattaaatt atattttaaa aatgctaata
     4321 cttcaaatat aaatattttt gtttaagtat aaaactgatt tcaataattc ggattttata
     4381 tttaatagat tgtgttactt aaagttaata aataaattag aaagatgtat ggtactaaaa
     4441 tctagtaata agttaaagta taacagcact ttagggttaa ttttgtagta agtatataaa
     4501 gtcaatatac taaattacaa taaaattgcc aaaaagccct ctcctttagg tgtagggatg
     4561 aaaggtaagg tcttaatata aaaaaagtta gaataatgct tgtaatctaa taccactact
     4621 gataccctat atttaggagg tgttagtttt tatgtataaa tctaacgaaa atatagttta
     4681 tgattgcaaa tatcatattg tattttgccc taagtatcgt aaacctgttt tagttggcga
     4741 agttgaaaag atgttaaaag aaatactacc atataaagca gatgaacttg gagcagaaat
     4801 aatagaaatg gaaactgata aagaacatgt gcacttatta atatcttgtg accctcaata
     4861 tggaattcat aaagtagtaa aaggtttaaa aggctttagt tcgagagtat taagagagaa
     4921 ctttccacat ttaaagtcta gtatgcctag catgtggact aatagttatt tcgtaggaac
     4981 tgttggctct gtaagtttag aagtagttaa acaatacata gacaatcaac caattaggag
     5041 taagaaaaaa tgataaaaac tcatagagta aaattaaacc taactagaac acaatttgaa
     5101 ttaataaggg aaaaacaaat ggagagtgct aattgttgga atcatatagt taacttatct
     5161 aaagaatact attttgaaca taaacaatgg attaaaaaga atgatataca aaagtcaatc
     5221 aaaggtaagt acaatcttca tagtcaaact attcaagcta tatcagataa atttgatgca
     5281 aatagaaaga ctatatcaga attaagaaaa aaaggtaata ctaaagcaaa atatccatac
     5341 aagactaaga aattttatat aatacctttt aaagctagtg ctattaatag aaatgctaaa
     5401 ggcaacttaa aattatcaat gtcaaaaggc aggtatttag aattagattt taatgttgag
     5461 aatataaaaa ctgctgaaat agtatggcga aacggatatt atttatacta cacttttgac
     5521 aatgaattaa atggagttgt agccaaaggc accaataccg taggtgtaga cttaggggaa
     5581 atacattcga tagctagtgt aacaaatgaa ggtgtagggc ttattctatc taatagagaa
     5641 ggcagaagta ttaaacaatt tagaaataag atgtatgctt atatatctaa aagattaagt
     5701 aaatgtaaaa aaggtagtag acaatcaaag aagttatgga gattaaaaaa taagataaga
     5761 agtaaaacag ataatcagtt aatgaattta tatcatcaaa caactagaaa gtttatagac
     5821 ttttgtgtgg agcagaaggt atgtgaaata gttcttgggg atataaaagg ggttgaaaaa
     5881 gatactaaga aaaagaaaag attaaacaga gttaatagac agaaaatatc tcaaatggag
     5941 tatggtcgaa taaaggacta tataaaatat aaagcaaaag agcaaggtat tgaagttaag
     6001 ttagttaaag aaaactacac ttctcaaact tgcccaaagt gttctaaaaa acacaaacca
     6061 actggtagaa cttatagttg tacttgtggt tataaaactc atagagatat agttggtgct
     6121 tggaatattt taaataaaaa gcataaatat ggtttagtag attttagaat aaatcataag
     6181 caaccaataa atttaaaagt atctaccgtt tgagccatgt gtgggcgaat aactttaagg
     6241 tagtagtggc gaagacaccg tccgaagtag cttggctttg taacacacct ctgttgtact
     6301 gtttaagtat aaataagtgc gttagccacc aagaatcccc ttgctttaac tatggggagt
     6361 gtgtcaatta ttaatagacg taatattgta aaaaaatgat atagtttaga aagatatgta
     6421 gattaaacgt agaattaaaa gattaacata gtatgacaat cataatctcg tgtagtatac
     6481 ttaaattaga ggtgagtata tgacttggaa tgatttaaaa aataatgata taacatatat
     6541 agttgaatat attaatagta agttaaatga tagtaagagt ttgactaaag ttgctataga
     6601 gcttggtgta tctgaaagta gtataagaaa atatctcact aaaagaggtt ataaacgtat
     6661 aaatgatgag tatgtgttta ttggtgacaa tagtatgaca aaggtagtaa acaacagaca
     6721 acagagtaat aatgataata gatttaatat gacaatagaa gatgacaaca atcacaacat
     6781 agtaattgac aaccaattta aaaataatat aatctcttta gcaaaagact atgataaaat
     6841 acaagatgta ttaaactggt ttgagaataa agaagataca aatgtaatag aagttgtaca
     6901 ggatggtatt aaaattgatt taccttcaaa agatgctata agaactacag ttaggttgaa
     6961 taaagatgca tggaacttat ttgatgagtt ttgtgaaaaa ttcagagagt ttaataagtc
     7021 tgatttgatg agcatggcat tattagaata cataaagaag tatgataaat aacatttttg
     7081 tattgttgta atcattacta ttttagggta atataaatat aactataata taaaatggag
     7141 gtattattat ggtcgataaa ataacaatag atgaagtact tgaatttata aaatcaaaag
     7201 gttatatata tagtcaagaa attagaaaag aattagataa tctttgtccg gtttctgata
     7261 atgaaataga taagtgtata gaagaaaatg atgatgtatt cagaaggttg gcaagtaaat
     7321 gattacactt gaatatatat gtaaaattaa caaaaagatg attgaaagat atggtggagc
     7381 tggaataggt attagaaata aaaatcttgc tataagtata attgatggca taaaccagga
     7441 ggtatttggt agagttatgt atcctacagt tgaagaaaaa atatctcata ttgtagtagg
     7501 tattatacaa ggacatatat ttatagatgc aaataaaaga actggattag ctacattact
     7561 aatactttgt gacaaatata atatagatgt gaattatgat aaagaaaatt tagctataga
     7621 aatagcttta aataaaataa atgagtctga tttgagtaat atgttaaaaa agtggaataa
     7681 aaaataaact cattaaatgt gttatttgtg ttatgtaaat ataaatctaa gaatttattt
     7741 taatataaat aagaatgacg ataaaatcgt agttttaatt taaataacct cattattttg
     7801 aggttatttt tttattttct atttaaaacg gagaaggtaa atgttataat atatatataa
     7861 ttaaaaaaag gatgtttgaa tttatgaatt ataatttaaa aaatgaaaat gtattatcag
     7921 agctattaca aaatgaggat gaaaaaacaa ggattggtat actcaaagtt tataaagtgt
     7981 taatattttg gtttgctaaa gaaggaaagc ttgttgagga aggaaatgac tatattgttg
     8041 agatagtagg taatgataaa gaaaaaattg taaaagatat gaaccagaat aaaacataca
     8101 atgccataat ggctataatt tttaaaatta ataagatatt taaaacttta aattacgata
     8161 ttcacttttc taaaccaaaa aatttagtcg ctgaatttga tttatcaaaa tatctaacac
     8221 aagatgaagt ccttcaggta tgtagtaagt taactaatgc acaagataag tttataattt
     8281 atgcagcttt ttgtgggctt attggtaaag atgctgaaga cttaaggagt ataaaaataa
     8341 aagacataga tttagaaaaa agattaataa agttaaagga tacagagttt aagctggaca
     8401 agttcttatt tgattttaca aaagatacat tagaacaaaa agagtataaa caatatttag
     8461 agcagggtag taatgctgct gaattttatc tatttaattc taattctgaa tacttaatta
     8521 aaactaaacc ggatgctaga tatggaaatg gattaaaaga gatgggatat tcaacaattc
     8581 gcaaaagatt acttattatt aatgattatt tagataataa agtacagttg aatttcacta
     8641 atttaaaaat ttctggtatt atgcatcaaa tgcagctaaa tgaaaaaaaa tatactcaat
     8701 ctggatattg gactaataaa agcttgaaag agtttaagga ggataacaat ttaaacttca
     8761 aaatacaaga agttctatct atttataagc aaaaatatat taaggagtaa ccttatgagc
     8821 aaagatgtta aagagaacat aagtaatgaa caaaaagatt taaataaaat aaatttaatc
     8881 tatcatatta tatatgataa tgatagattt aaaatgataa caagaaaata ttttgaaatt
     8941 tttagattaa agggtatgtc tgctgaagaa ataagtttaa aatttgaaga atttctatat
     9001 tctcttattt cagataagtt taaaaagata aatacagtaa atgatgttaa aaaattaaga
     9061 aatcaagaga actcatcatc attaaaactt aagaagagat ggtctgatat gactttagat
     9121 tctctaagtg aagatactaa atcttcaaga aaaaagagca gtaaatacct aaaaaaagaa
     9181 ttagagattg ttgaaagtga gtatttaaaa aatggaagaa tcattagtgc tggagtaaaa
     9241 aatccaaaag aattaaagga gcaaagaaaa gattttttag atgagtggaa gtcaagctct
     9301 aattataaca tatgtgatta cccatattta caagagctaa gtactagcaa aataaattcc
     9361 gcatttatac atgatatact aatgtgtata acagaagtac taaaagatga ttatgatttt
     9421 gatattaata aaattgcaat caaaactcca ccacatattt cacctggaat ttttttacct
     9481 ttcacaaaag gtaaaagata ctctcacttg aatgttaaaa aaaatgaaaa tgaatacatg
     9541 tcagacgact atagtgctaa gaatctttca ggtaaacaaa tgaatttttt ctataagttt
     9601 tcaattgggc aagatgaaaa tattgaaaat cttaaactag agctaataaa tcctgataca
     9661 gaaaaaaggg ctttgccaaa aatatattta gataaacaag accttgaaat tattagattt
     9721 gtttatactt atagccacat gaactcattt tcattttatt taggcgattt agtaaagtac
     9781 ttaggacttt cagatggaaa aaagaactat attaatatca aaaatagact cttaaaatta
     9841 ccttattata ctttttattc gaatcaaact aatgataaag gcgaattaaa gtatgagatt
     9901 agttttaatt tgttctctag tacaacaata gtaaatgatg aaaacaataa taatagagag
     9961 ttggttaaaa tcacaaaatc ttttattgaa gaagtcgaaa gagttaatac agatataatg
    10021 tatagaaatg aacttgaaaa attacaatta acacagtcaa ttaatctagc ttatttctta
    10081 gagggtagaa ggtcttttct tatatcttgt ggtgaggata taaaaaataa aagttttaga
    10141 tatgacatag aagatttaaa atttgatgtt catcttaata aaagtaaaac agtaaaacaa
    10201 aatatggagt atttagaact aggttttaat gagataaaag aaaatcaatt tataattaaa
    10261 gactataaaa gaggaaattc ttattttgat gtttatttct atgaagattt tgaaaaaaga
    10321 aaacgtctta taaataatac tatactttct ttacctgact acatgataga aaataaatct
    10381 taaaaatggc catatattct ccgtacttaa caaaaaatgt cgaatttatt ttaaataaac
    10441 ttaataaaaa aagtaaaatg accgtatatt ctccgtactt aacaaaaaat agatttttca
    10501 aaaaaactaa atgaccgtat atcttccgta cttaacaaaa aatagacctt tttttaatta
    10561 tttccttgtc gaaaaatttt ttttgtaaca tattttttac ttttcttagt aatttcaaat
    10621 ctcacgtctt ctgaaatcac tatgtctgag ctaaatgacc gtatcagctc cgtacttaac
    10681 agaagacgtt gaaaccactc tatttgagct aaatgaccgt atcagctccg tacttaacaa
    10741 atatagacat aaatttattt tttgggtaag ataaatctga gtaaaaggaa tcatcaccaa
    10801 aatggctata tcagctccgt acttctgaaa ttattgattt ttcaaaggag aaaattatga
    10861 gtcttaaata taaagataaa gaatttgtac ttgtagaaaa aaagaatata aatgaattag
    10921 ataactctaa tataaactat atcgacctaa aggacaagca gtattatgta gttacacaag
    10981 gtagaagaag taagagattt aataatgaag atgtaagtga aataaaaaaa gacttagata
    11041 atggtatgag tttaagaaaa tgtgctgaaa aatggaattg tagtacaaga accatacaag
    11101 atataaaaca aaataagtac taacgaggta ttatttacat tttacctcat taatattata
    11161 atattcttat actataaagg agttgattcc tatgaaatgg tcagaggtta gaaatttata
    11221 tcctaattca tttgtaaaat tagaaatttt ggaatcacat atacaaaatg aaaaggagta
    11281 tatagatgaa gtagctctta taaaggtcat tcctaacaat aaagaagctt taaaagaatt
    11341 caacagatgt aaagataaag aaattgtata taacaccaag aataaagaat ttgtaataga
    11401 tatcataaaa catattggta taagaagagg tttgtagtat gaaaaatttc aaatataaaa
    11461 atggattatt atatacagat atagaaatct taattgaaaa taaaaatatc cttgtaaaag
    11521 atacaatcat tgatactggg gcatctcaca ctataatatc atcttcttat ttagaagaat
    11581 cagatattgg atttaatgat gatgatacaa ttgtaaaggc ttctggttat ggtggaactg
    11641 ttcaatattc agtaaggaaa ttagttaata aggttgcttg tggtgacatc actcttgaaa
    11701 atataaaatt agattttgga gagattgacc cagaagaaaa agtaaatggt ttattaggat
    11761 tagatttttt aagaaatgct aatttgatta ttgatttagt agataatcta ttgataaaaa
    11821 aataaaacat gtttatataa ggagttatca caatatgagt attaaatata aagataaagt
    11881 agtctttatt gtagatagtt cggagaaaga gaagctagat aagtcaggaa ttgcatatga
    11941 aactttagaa aatgaaaatt attatgtagt ccaacaggga agaagaagta aaagatttaa
    12001 tgatgaaatg caaaatcaaa ttagaaaaga attagaaaat agtactatta gggcaattgc
    12061 taaaaaatat aagacaagca cagggataat acaaagtata aaagatttta aatactaatt
    12121 ttgtttcttc atttacaaca agaaacaaaa ttagtataat aagtttatag gaatattata
    12181 acttttaata ccaaggagcg aattatgagt attaaatata ataatgaaaa agtatttttg
    12241 gtaagtgaaa atgataaaga aaaattagaa aaagctggta taaactttgt gagtttagat
    12301 ggaaattatt atgtggtttc tcaaggtaag aaacctaaaa agtttacaga taatgaaata
    12361 agagaaatta aaaatgattt agacaatgga ttgagcataa gaaagtgtgc tgaaagatgg
    12421 gactgtagta caaatgtaat catgaaaata aagcaaaaca cttactaatt gtgtttttac
    12481 ttaataaaaa cacaattata tgttataata tacctatgag attatatagg ggtgatttta
    12541 gatgttagaa aatttgataa aagaatactt tttagattcc ttttgtattg aaaatattaa
    12601 tacagatatg aagaatttag aaataaaaat agacaatata tataattgct taaataatac
    12661 tttaaatgaa aatcagagga aactattatc tcaatttgat gaattactta aaatttagaa
    12721 aaggaggatt aaatatgcaa gaattaaaac cagttgctag aatatcaaaa tcaaaattaa
    12781 aagatatctt agatattttt aaaacttcta taagaaaagt agaaaaaata gattattctg
    12841 cttgtaattc ttgtgcaata aaaaataatc cttcttttga tagtaataaa ttaaatgaat
    12901 tttgttcaaa ttgtcctaaa ttaaaaacta tatcagagtt ttcttatgtt gcagaaaaag
    12961 aaacagaatt aggtatgata gagattgtaa ctaatgaaag taaatgtatg gctttaagta
    13021 aaagtgctat aaaacagtat ttagcatatc attttatagt atctaataaa aattatgtta
    13081 gaaaagcagt atccttccaa aacatagccg atatatgcaa tgtttctgtt gttacagcca
    13141 gacataacca taatgtatta ttacaagttg gattagttta ttccacagaa ataaaaagag
    13201 gtaaattcga tatagtcata gatgaagagt ataaaaatca tcttaaaaaa gaagatggtg
    13261 gaaaaggtta cttgactatg tcattagata ctttaaatca cttattaagt tttgaaaatg
    13321 taaatgaatt aaagcttgaa attaaaaagt taatttgggc tgatgctaaa ggaagtaagt
    13381 taggtaaaag aatacgattt aaaaaagata atcttatctc tatacttcca gaatacataa
    13441 agaaatcaaa caagcgtata gaagcctttt taagaagttc taaaacttta tttgatgtaa
    13501 gtaatgacac aatagataca aagcgttatg agacgaaaga aactatctca gagaaatata
    13561 cagaaccaat tagaatacaa atatcgaaat tctttgataa tactggttgt tctttgagtt
    13621 ctaattatag taaagtgtta tctcagataa atttagatag atataattta tttgaagaag
    13681 atttaagaag taaattagac atggaaaaag atattattat aaatgactta gttggattgt
    13741 cggtgcagta tggagttact agagttttag cagcattaga ttatatgttt aataaatata
    13801 cagttgatga agaagatata aattataatg aaatacgtaa tcctggtgca tttataagaa
    13861 cattgattgt tcagagcata aataaaaatg gaagtttagc aatagcaatt taatatatgg
    13921 agtagttcaa aaaaaatgat tttggctact cctattagtg tttgtattca aattataaaa
    13981 tacttaaata gagctataaa gagcgtgaaa tctctattag ctctattttt ttatgcctat
    14041 tttaatgaaa ttatatacac aatagaaagt tttttataca tattactaat ttaattttcc
    14101 aataa
//
LOCUS       DAENCV010000095        13926 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAENCV010000095 DAENCV010000000
VERSION     DAENCV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.096"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(396..2063)
                     /locus_tag="KQD82_002811"
     CDS             complement(396..2063)
                     /locus_tag="KQD82_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG4381271.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(2137..2892)
                     /locus_tag="KQD82_002812"
     CDS             complement(2137..2892)
                     /locus_tag="KQD82_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG4381272.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KDRISKLGLSIGIHLPMELNTCENIEYIRDSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2902..3438)
                     /gene="lepB"
                     /locus_tag="KQD82_002813"
     CDS             complement(2902..3438)
                     /gene="lepB"
                     /locus_tag="KQD82_002813"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG4381273.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3604..4140)
                     /gene="lepB"
                     /locus_tag="KQD82_002814"
     CDS             complement(3604..4140)
                     /gene="lepB"
                     /locus_tag="KQD82_002814"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG4381274.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4240..5550)
                     /locus_tag="KQD82_002815"
     CDS             complement(4240..5550)
                     /locus_tag="KQD82_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG4381275.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTRKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5629..6345)
                     /locus_tag="KQD82_002816"
     CDS             complement(5629..6345)
                     /locus_tag="KQD82_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG4381276.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6591..7862
                     /gene="sleC"
                     /locus_tag="KQD82_002817"
     CDS             6591..7862
                     /gene="sleC"
                     /locus_tag="KQD82_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG4381277.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8370..9026)
                     /locus_tag="KQD82_002818"
     CDS             complement(8370..9026)
                     /locus_tag="KQD82_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381278.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDDM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10299..10709)
                     /locus_tag="KQD82_002819"
     CDS             complement(10299..10709)
                     /locus_tag="KQD82_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381279.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10925..12307)
                     /locus_tag="KQD82_002820"
     CDS             complement(10925..12307)
                     /locus_tag="KQD82_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG4381280.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSSIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12318..12677)
                     /locus_tag="KQD82_002821"
     CDS             complement(12318..12677)
                     /locus_tag="KQD82_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4381281.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12882..13661)
                     /locus_tag="KQD82_002822"
     CDS             complement(12882..13661)
                     /locus_tag="KQD82_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG4381282.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4742 a         2522 c         1345 g         5317 t
ORIGIN      
        1 aacaacctaa taattcaata atttaacaac ctagtaattt agcaatccag caatctaata
       61 atccagtaat ttaaattttt ataacaccaa aaatttctaa cccaacacct tttactcttt
      121 tactctttta ctcttttact cttttactct tttactcttt tactctttta aaaaataatc
      181 acataaacta acaaaatatt aaacaaaaaa gtaaaattta aaataaaaag cttgcttcat
      241 acaaaagtat aaaacaagcc tttttatata tcaactcaaa ctttaaaatc tgtatcaatt
      301 taattctcca ctttacactt aatatactta acataaacca atcacactac ttgctatgcc
      361 atttaatctt cataaaacaa tctataatac acatattaat caactatttt acttcctcca
      421 ataaattccc taagaattga aatacctggt atgtcagaag tatcgtccaa ttcaacaaaa
      481 tactgcaaaa actttagtaa tctagtagct tcaatatgtg caggattatc ttcctttatc
      541 tcttccaaca aaggtttcgc aaatcgtttt aaaactgcca attcatatac acaagcagag
      601 ttaaatatag catctgcctc ctcttggtac gggaatatat tcttcttttc tcctcttcta
      661 actgatgccc attgtaaaat agtattatca gcactatatc ctctaaaatt atagtctcta
      721 acaattcttc ttatcattct caaatcagca gtagggattc gattatggtc atccaaattt
      781 atttgagtaa gggcacttat gtatatctta aatttatctt catctggtat agatgatgtt
      841 aaaataggat ttagcccatg tataccttct aatataattg gctgatttga atctatcttt
      901 atctttttac cagtgtattc tctcttacct tctttaaaat tgaatcttat atcatcaatt
      961 tcttcaccag ctaaaagttt ctttaagtct aagttaaact tctctaaatc tattgcataa
     1021 attgattcaa aatcataatt tccatattca tctaaaggag tatgttttct atctataaaa
     1081 taatcatcta aagaaattga aataggattt aaattattta ctctaagatg tattgataat
     1141 ctgtgagcaa aacttgtttt tccagatgaa gatggagctg ctattaaaac tactctctta
     1201 ttgccatcct ttatcatatc cgctatttgg gataacttct tttcatgcaa tgcttcaaat
     1261 gtccttatta tatctccata ttcattgttt tctattattt tattaagaga tactaccttg
     1321 tcaattccca taagtcttga ccattcttct gcttcataat atacacttga tagcttaggt
     1381 tgaggtacaa atttcatagg aagagtctta tcttcttcac taggtcctaa aattataacg
     1441 cctccattat aaagcttaat atcaaatgat tttatataac cagttgatgg caacatataa
     1501 ccataaaaat ggtctatata gtttctacac ttatatactt tagcactatc atactctttg
     1561 tatttcaaaa gctctgcctt ttcatacatt tcattatttt caaagataga aatagcttca
     1621 tttcttgtaa cttcaatttt ttctataaca taatcgttgt ttactatctc tttcatcttg
     1681 ttttttatat tttcaacatc tgcttcttta agctttctat ctatatgaac ttcacaatat
     1741 agcccatctg atagtgaatg ttctattgta actctgctat cccaaaagat ttctctacat
     1801 gccattacaa atataaatga catgactcta aagtaaactc tctctccatc ttcattagta
     1861 gtatctaaaa aatttatctc acaatccttc ttaactctac aatttagctc ttttagttta
     1921 ttgtctacaa ctgcaagtgt tatataaccc ttatgctcat cacaaaattc ttttgctata
     1981 tctcccaatc tgattccagg ggaattttct tctaatgaat attccttatc ttttccttct
     2041 atttttaagt ttattttttt cacttcaaca tctccttcct tttgatgaat ataaatttta
     2101 attaatgagg catatcaaca agataaagag aggtatttaa ccctctccta caatattatt
     2161 caatttactt atagtatctt caatgtcttc gtaatttatt tctaatacta aatattctgc
     2221 atctaatgtt aagacctctt taatcccctc ttcagataaa attcctcttc ctataccaac
     2281 atgtgtatca ttgataccat cattatcact taagtgtatc agtcttattt tatcctttaa
     2341 gttctttaca taagcatcct catcaattaa acagtgtcca gtatcgtaac aaaaacaaat
     2401 cttagaattt ttaatcctct taaatatata ttcaaaatca taagatatat ttccaatatt
     2461 tgaaaaatcc ccaccttttg aataagtatt ctctatacat acatatgaat ttgtatttaa
     2521 tttatctaaa aaatccacac tatttcctaa atatttgtct ctattctttc taactctatt
     2581 tgtcataaca tatcccaaat gaaggttaaa ataccttaaa tcaaatcctt ttagttcaaa
     2641 ttctatcttc tcaataaaat ttatccatga atctcgtata tactctatgt tttcgcaagt
     2701 atttaattcc ataggcaaat gtatccctat agataatcct aacttagaaa ttctgtcttt
     2761 atatttacat aactcactac attcagatat attgtcaatt ccaatctcta aatgagttat
     2821 ttgctttata ctctcacaaa tgtttaaagc ctcctcaata ttaaacaata aggacgctat
     2881 accaactgcc atacaagtca cctactcaaa aatacctata tctgtaaaag gaaatactct
     2941 taaaataact tttcctttaa tattttcttc atctactaat cccacttctt tatatctact
     3001 gtctaaacta acttctctat tgtctcccat cgcaaatact tttcccttag gaatcaccat
     3061 atcaatatca ccttcagtat aattatcatg tatatatgaa acttcattta ataacttacc
     3121 atttatataa accttagaat ccttaatttt tagatggtca ccaggaacac ctataaccct
     3181 ttttacaagc tcttttttcc ttccattctc ttgtaataaa tcagtcttaa atatcattat
     3241 atctccacat tttggttctg ataacttata tgtcatcctg tttgctacca aataatcatg
     3301 tgatttaagt gttgggtaca ttgattctcc atttactaat gttggtttta taaaacgagt
     3361 tataataaat gcaaaaaaaa gtgcagtaat aattattttt atccactcta caatctcttc
     3421 tttaatagtt tcattcattc acaaattctc ctttatagtt atattatctt tattgataat
     3481 gtctcttaaa cactatcttt tagataagtc tactaaataa ggtgttgtct tttttatata
     3541 gacaactatt gtgtatggtc tggtgatttc agctaatcag cgattgctcc aaattttata
     3601 tgtttagtca atagttccta tcttagaaaa tggatacaat ctgactagaa cctttcctaa
     3661 aatggtatgc tcgtcgacca atccaacctc atcgtatcta ctatctaaac ttttttctct
     3721 attatctccc attgcaaata attttccttc tggaactacg atatcaatat ctccatcagt
     3781 acgattatta tgtatatagg aagtctcatc taacaactta tcatttacat ataccttgga
     3841 gtcttgtatt tttacatggt caccaggaac cccgataact ctttttacta aatctttttt
     3901 ctttccattt tcctctgtta agtcggtttt gaatactatt atatctttgt attttggctc
     3961 tcctaccttg tacgcaattc tgttaactat caaataatca cgttcaacta atgtaggata
     4021 cattgattct cctttgacta ttgttggcac tataaaacga gttattgcaa atgccaaaac
     4081 aagagctatg acaatcactt ttatccattc tacaacttct tttttaactg cttcacccac
     4141 taaaatcgtc tcctctgtgt taaatattaa gtcaatctac agcaaaataa tctaccataa
     4201 acctacaata taaatattat acttcttttt tctaatagtt taaaatactt ttctaagacc
     4261 tttaggataa tgattcttaa ttacaccaga agattctttt ccccaaccta aaggtattcc
     4321 ttcaaccgaa accaaaaccc atcctctact atcattagaa tttacaacat ccccttttaa
     4381 atatttaaga attgattcac tctgtacact gaagttttca acatttttta catcttcaac
     4441 ttttaagtaa tgagatagtg catgagaagg ctcaaatcta ttcttcttta aaacccctaa
     4501 atgcaaacca tatctcaata cttttagttt tctcgtatct ggatatattt caggaagtag
     4561 gtacaaattt tctcctctta aataaaattt atttttttta ttactataaa aatcaacctt
     4621 taaaaatttc ttttcaaact ccctataatc ctttatttct ttatctaaat tttttatttt
     4681 taattctttg acattacatt cttcttcttc taattttttt attttggcaa caaaatgacc
     4741 ttctcccttt atcttatgag gccataatct ttccatttca ataagttctg catgtggata
     4801 ttcactgata aactcattta taacatgctc attttcctcc cttgaaaaag tacaagttga
     4861 gtaaattaaa atgccatcct tctttaacat tttatatcca tctcttataa tctctttttg
     4921 gatggattga cattctacta cctttgcata actccaatct gatatggcaa cctcatcctt
     4981 cctaaacata ccttgcccag aacaaggtgc atctataata accttatcaa aatacccatt
     5041 aaacgtcttt cttaagctat tagaatcggt gtttgttata atacaatttc tcgctccaaa
     5101 tcgctctaaa ttttccccta gagccttaat tctactagca ttaatttcat tagaaaccaa
     5161 taaacctgta tcgtttagct tagatagtat atatgtagat tttccaccag gagcagcaca
     5221 catatcgaga actttttcac cctctttaat atctgccttt ggtattacac tcattgcaga
     5281 aggctcttgc aaatagtatg ctccagcctc atgcagtgga tttctaccag gtctgtcaat
     5341 tttatcatca taataaaaac cttcttctgc ccatggtatt tgattcaaat caaacaactc
     5401 caaatcaaat aattttttct tgtcaatttt taaagtattt attctaagcc cagtagtctt
     5461 actttcctca taacttttta taaaatcatc atattcatct tttagtattt cttcaatatc
     5521 ttctaaaaat tcctttggta attctgtcaa atctataatc ccccttcaat ctgctttact
     5581 tgtgtgtcat ttattatact ttaattattt atgtctttcc tttaaatttt atttcttctt
     5641 ctttttccta agattaccga aaaacacttt tttagagtga ttcatatatt tctcaagttt
     5701 atcaagattt atcttccaca aaattattac ataaactata agtaatataa tagcccatct
     5761 atattctatc atcaatatga aatcaaaaac tccatgtaat aaaattggaa ttaacactgc
     5821 cataaacaaa tactcacgtc ttttattttt atttccctca aatttatact tagaaatata
     5881 gtagcccata gtaattgcaa acattacatg agctggtata gagataactg ccctattaat
     5941 accaacttgc agtgctagat ttctactttc agaaaaaata taaaccatat tttcaattgt
     6001 tgcaaatcct aaagctaaaa atacagagta aataatacca tctaactttt cagtaaagtg
     6061 tttttcttta attagggcag gaatcagtat cagagctttt acaccttctt ctacaagtcc
     6121 tgcaactaca aatgatacat atattagttc tgaataccct tcaaatacat taaacttcat
     6181 cagtaaattt tctaaaagta taattataac acttagaaaa gcacctatta aaaaaaattt
     6241 aattagatgg attaggggtt ctctgcaata tctatcttta gaccttatcc aaaacatccc
     6301 tatcaatata ggaactattg ctaataaaaa taaatctagc ttcatagatt attttccttt
     6361 caaaattttt gatttattta tgatttatat catctactta tttttaactt attcgtgttt
     6421 taaatacata ctaaaatata cattaaattt gatataaatt atttaaatac aaatatactc
     6481 aaatactcaa aaaaatttaa atttatatct atattattta acaaatttca agctatattc
     6541 atatcttgaa atataaggga actaaattca tttaaagaaa gggtgatttg atgcaagatg
     6601 gtttcttaac agtaagcata attgatgcta ctaacaatag acctattcaa aatgcagtag
     6661 taaacatata ttcaatgtct aatggtagcc aaagctcatc aactttatac caaaacctaa
     6721 gaagtaatga atctggtcaa gtgactggtt tagtattacc agcaccagat gtagattact
     6781 ctctacaacc atctgatgtt agaccttata gccagtatat agtggaagcc atagcagatg
     6841 gatatgagac agttgtcata gaaggaacac agttgttagc tacaattgaa gcaagacaag
     6901 gagttcccat gtctcctaga acaagaagta agcgttcttt ctcaagacaa agtgagttga
     6961 tttttgatat aggagaacat actctatatg gtacttatcc tcctaagatt ccagaaagta
     7021 acttaaaacc attacctcct cctactggtt tcgttgtatt agataaccct gtagtgcctg
     7081 aatttatagt agttcatgat ggtttgccag aagattcatc agctccaaac tattggattc
     7141 catttaaaga gtacattaaa aacatagcgt catctgaaat atattcaact tggcctgaac
     7201 agacaattta tgcaaatgta atagctataa tatcatttac tttaaataga gtctttactg
     7261 aatggtatag aaataaagga tataacttta caataacttc aacaacagca tacgaccata
     7321 agtttataaa taatagaaac ttatttgaac caattaacgt agttgtagat gctattttta
     7381 atacttttat aaaaagacct cctacatcaa gacagccact attagcacaa tattgtgatg
     7441 gacaaaaatc ccaatgtcct gaccaaatga ctcaatgggg cagtaaagac ttaggtgacc
     7501 aaggatatga ctatgagagt atactaagat atttctatgg agatgaaata gtatttgaaa
     7561 gagcacctat agtaagtggt gtaccagtgt cattccctgg tactacacta caagttggtt
     7621 cttctgggca gtatgttaga accatacaaa atcagttaaa tgctatatct aattcttatc
     7681 cagctgtacc aaaagttata gaagatggta tctatggaac agatacagaa aacgctgtta
     7741 agatattcca aggaatcttt ggtttacctc aaagcggagt agtagatttt aaaacatggt
     7801 atgaaatatc cagagtttat gtagctacaa ctagaatagc ttctttaaat cctttaattt
     7861 aaagcttgat ttagaacata aactcaaatt attgaataaa attattaatt taaaataaaa
     7921 atggctgtta tgaactaata tataccataa gtattactta taagtattgt agtcttaaca
     7981 gccatttttt agttataact tgttttttta gaatcttaaa actttaaatg ggggatttta
     8041 agcttcttaa aaacagtgct tagcaactta gatttcttta aaatattata tttattttta
     8101 agtttaataa aatgctattc taacgaaaaa cctacttttt atttaaaatc tcacttatta
     8161 tttaaaatgc tacttctcac ttagagtatt actccttatt atttaaaaca ctatctatca
     8221 cttagaatat tacttcttat tatttaaaac actatccatc acttagaata ttacttctta
     8281 tataaattat tatttattat aaatacaaat aatattaatt taaaaagtta ttcctaattt
     8341 aaataatact actatcttca tcagtgaacc taaactgcaa ctttagactt attagctaaa
     8401 gccaatatta gacctaaagc tatagatgca aatgcagaag caattactct atactcagca
     8461 gggaataaga atacaattgt atgacatggt atccagaaaa atggtatagt ttttaaaaca
     8521 ctaaaaccaa ggtaagcatt ccagtccaca atatttacca catctttaag ttttacctta
     8581 atcccctttc caacctcata ttttttatct ataaaacagt ccataacctt atggaataaa
     8641 aatacagttg gtgacattag tatattcata gttgcactct taaatagagc atttgcaaaa
     8701 gctattccct caaatggaag catatcatcc acttgtgctt gacctacacc tgatgagtag
     8761 attgacattc catatgtggt aactacacct attattcccc aaactattgc tctataaaat
     8821 actcctactg gtattttcca atccttatac aaaattctag caccaagtaa atctcccatt
     8881 gtagctaata cagcaaactt taaaaaacct ccaatatatg gatgtgcacc tgttgcttcc
     8941 ataaatactt ctctactact tggtattgct aagacagcaa taatacaaat aagaactcca
     9001 ctccatataa aatcgccttt tttcatattt ataaatacta tttaatatat aaatagttct
     9061 cctttctatt aaagatttat ttaatttaca atctatctac aattatttat taatcactta
     9121 ttattagata ttcattttcc atatttaaat ttattctatc cctttatcta tttattttct
     9181 tactactcta tccaccatta tctatatatt tattttacga ttactctttc accctaaccc
     9241 tttaattatc tatttttttc tgatattctt atctaccatc atccatatat ttattttacg
     9301 attactcttt caccctaacc ctttaattat ctattttttc tgatattctt atctaccatc
     9361 atccatatat ttattttacg attactcttt caccctaacc ctttaactat ctattttttc
     9421 ttactattct tatccacatc atccatatat ttattttacg attactcttt cactctaacc
     9481 ctttaactat ctattttttc tgatattctt atccaccatc acccatatat ttattttaca
     9541 attactcttt taccctaacc ctttaactat ctattttttt ctggtattct tatctactat
     9601 tatctgtata tttattttat gattactctt ttaacctacc ccctatactt tatagtaata
     9661 tgtttttaac ctttttcatt attcccaata tgctttataa ctacaaatac tctcccccct
     9721 taaaaataat aataaaatta atcaataaat ttataatcat attattactc tatgtattaa
     9781 gcaattacta tgccaataaa ctttgatttt tttaaacaaa tcttaaaaat cctaaaatgc
     9841 taattttctt taacttttaa tgaatctaat agataacatt taattttcgt tatatttttc
     9901 agaatatttc tattattatt tttaagaaaa aaaaataatg ttaacatttc ttaacataca
     9961 ttaaaaaatg ttaacattat aaattgggtt taatactttt actttgactc gatttacttt
    10021 tattctactt cttactttat ttttaactta gactttattt ttttattact tatttgagtc
    10081 ttatttattt tattctcact ccatatgttc aattacattt gtctaaattc tttgatactt
    10141 ttattacata attttaacaa gcttaagtgt tatttgggta gtgcagttga taattattta
    10201 aataatcatc aactgcatgt ttatatcatt acagtagctt aatgtattta atcatactta
    10261 cttaggattt tgaaatatct tagagattat atttaacatt agtcaattgc tgcataattg
    10321 taagcaattg aaatttgaga cccatcttca ttttctatag tgataggctt cataattttt
    10381 aatccttcag tttttatctt ttctaattct tgttccattt tcttaattgt ttcctcaagg
    10441 tctttttgac ttacatcttt gactgcatta gggtctttag catagtcttt taattcttga
    10501 atatgtttag catactcttc ataagtataa tgctcaacac catacttttc caattcagta
    10561 gggtcagtta ttcctgattt tattagagat tcaaatttag gtaaagcaat attagatgaa
    10621 tcatttccat ttgcaaatac tgctccagtt cctcctagta ccattgcaga taccatcatt
    10681 gcacttataa gggatttttt tattttcata accaatcttc ctttcttaga gatttatttg
    10741 ggtagtgtag tttaaaatga ctattgcaat agttatcaac tacactctta gactatcaca
    10801 gtagtcatgt ttatgtcaag cctttttaaa aaacttttca ataaaaaata ctatctcaaa
    10861 gcaaaattaa ttattttgag atagtatttt aattctaaat cttttatatt actaagattt
    10921 atggttattc tatccatgtt ctatattgtt ctttattaac tgctaacaaa ttcccttttc
    10981 ttgatatgac aaagcgatta tcattatagc cacttcgctc aattgaaact tctcctgaat
    11041 cagtataaag tacatcctta ctattactag atttatttac atacgtttct tcactattct
    11101 cagaattagt tactttgctc atagatgatt cttcatgctc tctaaattca attacagata
    11161 cttttgccgt actaaaatga gtctctccat cacttttagc tttactaaca agaatatcag
    11221 attctacttt gggagaatat aaatcagttg atttactact tattgaaaat ccaattttat
    11281 cactttgttc attttttgtt aaaccaaaag attcaacctc attaatctct ttattttctg
    11341 ttgcaaaaac aagacctgtc ccacaaacta atacagttga aactaaaaaa gtaacataag
    11401 ttgatttttt aaatttcata atattaacta ttctcttttc tatttttgtc tttccaaatc
    11461 cactacatat tgtcattgaa aatggattat tctcctctgc tagacttatt agtgtggttg
    11521 cataatcagc cctatattct tcaccaagta tactcataac cttttcatca caactaaatt
    11581 ctatgtcttt atttagtaaa aaaaacatca cccaaactaa gggattaaac caatggacaa
    11641 caagagctac aagcataaat aactttaaca gattatcaaa gctattaata tgaaccattt
    11701 catgagttaa aacaaagtct aaagtttgat tattagcata attcatttgc tttggcaaaa
    11761 taattcgtgg ttttaaaata ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa
    11821 caataaatct tctaaaacgg ctattcttac acatccattt tttaatgaac tcatgatttt
    11881 ttatatgaat tgaagtttta aaaagattaa ttattttaaa atatctaaag cataaaagta
    11941 tgctaaacat aatagtaact ataaaagaca aaatgcccaa catttccaac atcataatat
    12001 tttcaatact agacattatt tcaaattcgt cagaattgtt tttacctgta gagtcactag
    12061 ctttcttaag agtataattt tctgttgatg tattacccaa ttccacatca aaagtcgatt
    12121 caattatact ataaatacta aaaccagaat ttaaacttac tggtattaga agtttaaata
    12181 aaacaatcaa ccacaataat acaaatacct ttttaggtaa ttttttaaat aaacatgctc
    12241 ttataaaaat aactattaaa agtaatactc cagaagtaaa actcatttct aacaatttaa
    12301 tgaacataaa tcttacctca ctttaatttt tcaacaattt gtttaagttt tgtaatctca
    12361 tcacttgaaa gattttcttt attaataaaa gctgaaagaa atttttctga agaccctcca
    12421 aacattttat taattaaagt ttctgtttcc tgtttttgta cttcctcttt gcttattaaa
    12481 gctttacaaa caaaattcgg ctcaattctc tctattgcac cttttgacac taattttttt
    12541 ataacggtat acgtagtgtt tctattccat cctgtttctt cttttaaaat ttttactaac
    12601 tctccagcct tgctttctcc tctctcccat agaacttcca ttactttaag ttctgaatca
    12661 aataatttta tctccatata aaatcgctcc tcaaatatag ttatcacaat agtcattact
    12721 ataataaaaa actatcatag tagtctatac tatgtcaatc atttattttt taataataaa
    12781 aactgtcttt ttaaaaaatt cttataaaag acagttttat caatgttata ctttaatgtt
    12841 taattaaagc catattttat ttaaatatat aactatctat cttagtacat agtataagat
    12901 ttttctttat ctaatagtat atcctctaca tctgggtttt cttttactaa ttgtctataa
    12961 aaattacaat tagtaacacc cacatatgga attagccata gtagacctat tccaagagta
    13021 gctattgaaa gtatatacca tcctataaag ctaaagttta gtacaaataa tttaagtaca
    13081 tttcctttca tcatatctaa actagcaccc attgatttaa atacacccat attatccttg
    13141 tcttcacaaa ttaagaaagt agcttgagaa acatataata gcaatatata aactggtaac
    13201 ataagaacaa gtattataag aactagagtt attccagtag aaataatagt ccctattcca
    13261 acattatcac ttgataaata ttcaattgct ccactagtaa gtaaaactcc aaatgataaa
    13321 aacgctccta taattgtgat tataaaagaa taaatataca caataattga ttcaaataaa
    13381 taaaaaccaa aacttcttaa ataaattctc ccacttacaa agatatctcc tatcttgact
    13441 ttttcatctt tagtaatttt caagaacata catgtaaatc ctgctgaaac gcaaaaagct
    13501 attacagcca ctacaatagt agtcaatggt tcaaaaggta atcctacaat tgatattaat
    13561 actatcaatg caatatatac tgccatagaa attgcacata tcccccagtt tccagataac
    13621 tgagctttag atactcgttt taattcttgc cttgatacca taaaaaacac ccctttcatt
    13681 ttataaaaaa acttttaaaa atataaataa tcttttaacg tataatatta atatatcata
    13741 ttttcgacta tataagatac aattattcac catgttttat caagatatgc cataattgta
    13801 ttactagttt tatatagtca acattaccaa atacatcaaa taaatttgat taattattca
    13861 acaagtaata aaataatttc tattaattat actttattta gtaaacaaat tatttaataa
    13921 aaaaat
//
LOCUS       DAENCV010000096        13805 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAENCV010000096 DAENCV010000000
VERSION     DAENCV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.097"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            189..329
                     /locus_tag="KQD82_002823"
     CDS             189..329
                     /locus_tag="KQD82_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381283.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(504..824)
                     /locus_tag="KQD82_002824"
     CDS             complement(504..824)
                     /locus_tag="KQD82_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG4381284.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(944..1681)
                     /locus_tag="KQD82_002825"
     CDS             complement(944..1681)
                     /locus_tag="KQD82_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG4381285.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1896..3719
                     /gene="acd"
                     /locus_tag="KQD82_002826"
     CDS             1896..3719
                     /gene="acd"
                     /locus_tag="KQD82_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG4381286.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3934..8232
                     /locus_tag="KQD82_002827"
     CDS             3934..8232
                     /locus_tag="KQD82_002827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG4381287.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8436..8897
                     /locus_tag="KQD82_002828"
     CDS             8436..8897
                     /locus_tag="KQD82_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG4381288.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8927..10081
                     /gene="nusA"
                     /locus_tag="KQD82_002829"
     CDS             8927..10081
                     /gene="nusA"
                     /locus_tag="KQD82_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG4381289.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10088..10369
                     /locus_tag="KQD82_002830"
     CDS             10088..10369
                     /locus_tag="KQD82_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG4381290.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10359..10670
                     /locus_tag="KQD82_002831"
     CDS             10359..10670
                     /locus_tag="KQD82_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG4381291.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10692..12632
                     /gene="infB"
                     /locus_tag="KQD82_002832"
     CDS             10692..12632
                     /gene="infB"
                     /locus_tag="KQD82_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG4381292.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12675..13055
                     /gene="rbfA"
                     /locus_tag="KQD82_002833"
     CDS             12675..13055
                     /gene="rbfA"
                     /locus_tag="KQD82_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG4381293.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13036..13713
                     /locus_tag="KQD82_002834"
                     /pseudo
     CDS             13036..13713
                     /locus_tag="KQD82_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5512 a         1560 c         2589 g         4144 t
ORIGIN      
        1 ataataatag ttagattttt tatatgaatt ttaataggta aaatatccta ttgttttgta
       61 gatatatcct aattatgtca aaaaataaaa aaatttttat ttattagagt ttttggaaat
      121 aataataaca gatattataa ttaattaaca gtttttgtaa gtatttgaca atagagaggg
      181 tgtaacaaat gggaataata gagtcagcat caaaattggc tgagatggtt catttattgg
      241 ctgttgaaaa ggggataact gatatagaag catgggatga agctgtgaaa gaatacagca
      301 aaatttatga agaaagaaga aatgaataga atgataaaaa atgaaaactt gatgataaaa
      361 aataacttta tttaaagaaa ttcaattttt atagaagtat taaaaataat ataaataaaa
      421 agatggtatt tcaaatgtaa gtaaaaaatc attttgggat accatcctta ttatttattt
      481 taataatata taagtaataa atcttactta tccatatttt ttatgtaacc tcttttttct
      541 agctttttta tatgctcctt acgtatttca tcaagattaa taccatattg ttcttctagc
      601 ttaaacatca tagtaacaca tgtctgcgct acatccatta gttccttagc agttttcttt
      661 ataatatcaa cttcatctaa atcattattt tctccactta gatttctgaa tttacctata
      721 cattcagcta attcaccttg ttcttcaccc attttgataa atgtagattc tagtgttggg
      781 tctaaattat taagctctgg taaagatata gtttttagtt tcataaaagc tcctttcaag
      841 ttattcgtat ttatactaaa acatcataac aaaaataata ctaaaaaaaa actttaaatt
      901 caacataaaa acttacatat ataataaaaa tcgtgaaata aatctatatt tgtcttaata
      961 cagcctcata taaaacttct ccaataggtt tatttattac aagagaagct gacttatcat
     1021 agactgtatt tgctttatta ataagagcaa tataatcacc tttgtaataa tctataaaac
     1081 ttgctgctgg gtacacagct aatgatgtac ctcctataat caataaatca gcattactta
     1141 tagcagaaat tgttttagta atgacatcac tgtcaagagc ttcctcataa agtactacat
     1201 ctggttttac tatagaacca cagttgtcac agtaaggaat attacctccc agatttaaca
     1261 tagattctaa gtcaaaaaac ttaccacatt tagtacaata atttctatgt acagaaccat
     1321 gaagttctag cacattttta cttccagcca tttgatgaag tccatcaata ttttgagtta
     1381 tgacagcttt aagcttaccc atttcttcga gttttgctaa agctatatga gcattattgg
     1441 gttttgcatt aggatatatt aatttatcct tataaaattc aaaaaattct tctggatatc
     1501 taacaaaaaa agtatgtgaa actaattgtt cagcagtaaa ttgtttattg agtttttggc
     1561 taaataatcc agtagaactt ctaaagtctg gaatattaga ttcagttgaa acacctgctc
     1621 caccaaaaaa tacaatatta ttgtgattag ctattaagtc ttttaagcta tttgcatcca
     1681 tagttacacc tccaaattat tatatataaa ttgtactaca tattaggtcg tttatgtttt
     1741 attaatttaa ataaatatat agtttaataa gttaaataaa gggaatataa ataaaatgta
     1801 tacagagtat aaatagacaa tatttactaa aataacaagt tgtcgtataa ttatattaaa
     1861 attgttttag tgtataagaa gggcggggga agtacgtgaa gaaaaaagca gctttagcaa
     1921 cattggctat gttaccctta ggtgtggtaa atgcacatgc tgatggagat ataggtatag
     1981 tgactataaa ttatttaaat gtaagaaatg aaccaactgc cgaaagtagc atagcctttg
     2041 ttgctaaaaa agatgataaa gttttgatta aagactcttc taatggatgg tataaaataa
     2101 aagctgaatc tggacaagaa ggttgggctt catcaaaata tatagcaaaa tcaaatagtg
     2161 actctttaag aacgtctaca aataaagaaa aacaagttat ttcaaatagt ttaaatatga
     2221 gaaatggagc aggtacaagt tacagagtaa taactgtact aaaaaaaggt caaaaagtag
     2281 aagtaatatc agagagtaat ggatggtcta aaatcaaata tgatggaaga ctaggatatg
     2341 tatctagctc ttatttagga gacgtttcaa actcaactaa taaatcaaaa actaaacaag
     2401 tgaatacaac ttcactaaat gtaaggagtg gaccaaatac aagttatggt ttgttaggca
     2461 agttgccaaa aggaagtaaa gtagaagtaa tatcagaaag taatggatgg tcaaaaataa
     2521 agtataatgg aaaagatgcc tatgtatcta gtatgtactt atcggatgta agtcaaagta
     2581 attcagacaa ttctagtcaa agcaatgata aaaagaatac tgataaggtt gtaaatacag
     2641 cttctttaaa tgtaagaagt ggaccaggtt ctacatatag taagttggga aaagtttata
     2701 aaggaagtaa agtaactgta ctatcagaaa gtagtggatg ggctaagatt aattttaaca
     2761 ataaagaagc atttgtagta ggcaattatt tatctacttc agcagatact tcaaataata
     2821 actcaaatag taactctgat aacagttcta atagtaatgg caataattct tcatcatcag
     2881 gtcaagtaaa tggtatgtca ggtataagtg gggctaaaat tgattataaa tctttgagtt
     2941 atactttaga atctcatata agtaaacaag ttgagaaagc agcatcagga ggaaatgtga
     3001 tagctccaag taataggaaa agcactccaa gtcctgaatt tagtactttt tcagctcaaa
     3061 gaacaagctc atttgtaaat gcaagttcaa gtgatataga atattattta aatcctaaga
     3121 attttacaaa tactactaaa ggtatgatgc agtttttaaa gattaacagt tatagagatg
     3181 gtatttcaga atcaagtctt aactcgtatc ttaatggttt atcttctagt gtatttaaaa
     3241 accagggagc agcctttata aatgctgcca agaagtataa tatagatgtt gtatatctag
     3301 tatctcatgc tatgtgggaa actgcttatg gtaagtctac acttgcccaa ggtcaaacat
     3361 taacatctta taagggacaa cctcttagta agccagttaa agtatacaat ttttttggta
     3421 taggagcaat agataaaagt gctaatgttt caggtgcaga agcagcgtat tccaatggct
     3481 ggactagtgt agaagctaca atagatggct ctgcaaagtg gatatcacaa aattacgtaa
     3541 atagttctaa gtacaatcag aatactattt acaagatgaa gtggaattat gattatacat
     3601 ggcatcagta tgcaactgat gtaaactggg ctaatggaat ttctggaatt atggaaaatt
     3661 taattggtct ttatggagga ggaagtagtt tggtttttga agttcctcaa tataagtaat
     3721 acataaataa aaaatataga ctatgggtag ttttcatttt atttaaatta tctatagtct
     3781 ttttgtatat aatttataat ctgtaaattt aatgttaaca aaacttttat gttataatat
     3841 taattctaag taaatataag tgagtaatgg gtagaaatag ataaaaagtg gtaataatat
     3901 atataaagat aattttgaga aaggaggata tatatggaaa gtattaagga atatttggat
     3961 aagttagaaa ttaataatag tggtcttgga aagcaactta aagaagttta tataaataga
     4021 gtagtttatt ttaaggaaga taagatagtt tatttttatc taacttctaa agatattgtt
     4081 tctcatgaac ttttagataa atttaaagaa gaattgatgt ataaactcga ttattttaaa
     4141 gatatgagga tgaagataag atttactggt cttgaaagaa aatccaataa agatgttata
     4201 aaaaaatatt ggaataatat attgtatata ttaaagtatc tttgtccatc tattgcaggg
     4261 tggtataaac aagtagaatt tctttgttta gaagaagaat taaaaataaa acttccaaaa
     4321 ggtatatttt atgagcgatt aatgaaaaaa aatgtaactt atgtacttaa aaccgtgctt
     4381 agcgaagagt tgggattaga tttaaatata acaatagaaa aagctgttga tgaaaaggta
     4441 aataaagaaa gactcataag aatcaatgat agagaaatgg aagaaaaaat tagggcttta
     4501 gaaattggaa aagttaataa ctgtgaagaa aatgaagagg aaagttatgt cattaagtct
     4561 gatgtagatg aaaatttaat ctatggagat aatgcaaatg ctatgattga aaatatagtt
     4621 gaattaaatg catcttctgg aacagttgca gttgttggag atatatttga tgttgacact
     4681 aaagaactta aaaacggaaa aatacttatg atagcttcaa ttacagatta tactagctca
     4741 ataagctgta aacttttttt aacagataca aataaggatg gagtacttga aaatgttaaa
     4801 aaaggagcat acttaaaaat caagggtgac atagtttatg atacatatca aagagaaatt
     4861 agtatgatga ttagtgggat aagaaaagaa actagaatag agagagaaga taaatcagag
     4921 gaaaaaagag tagaactcca tgcacataca caaatgtctt ctatggatgc tatttgttct
     4981 gttaaaaagc ttgtagagcg tgcagccaaa tggggtcatt ctgctattgc tataacagac
     5041 catggtgttg tacaaggatt tccagatgct atgaatgcag gtaagtcaaa taatataaaa
     5101 atactttatg gagtagaagg gtacttggtt gaagatgatt ctctcattat agaggatgca
     5161 aatgataaag aactttcgca gacatttgtt gtatttgaca tagagacaac tggcttttct
     5221 aatacaaatg ataaaataac tgagattggt gctgtaaaaa tagaaaactt taagattgta
     5281 gatagattta gtgagttaat aaatcctgaa aaagatattt cttataagat acaggaattg
     5341 acaggaataa ctaatgaatt aattaaagac aagccaacca ttgaagaagt tcttcctaag
     5401 tttatggaat ttgttggaga tagtgtatta gtagcacata atgcagagtt tgatactgga
     5461 tttatatcgc aaaaatgtag ggaacaaggc ttgttatata ataacaaaaa agtagataca
     5521 cttatgttag caagagtaat gttaccaaac ttaaaaagac atagattaaa tgtcgttgca
     5581 aaagaacttg gtattcctct tttaaatcac catagagcag tggatgatgc tgaagctaca
     5641 gcacttatat ttaataagtt tttgcagatg cttacagaaa aaggtgcaaa aactctaagt
     5701 gatgtaaaca atatacttgg aaagattgat tacacaaagc ttagcacaaa tcatataacc
     5761 ttgatagcaa aaaattctat aggtataaaa aatttatata aaataatttc agatgcacat
     5821 gtaaatcatt tttttagagc accaagaatc cttagaagtg tattaaatga atataaagaa
     5881 ggattaataa ttggttcggc ttgtgaagct ggggtggttt ttcaagctgt aaagaaaaac
     5941 gtgtctgatg aggaaatgaa aaaaataata gatttatacg attatattga agtaatgcct
     6001 atagataata atagatttat gattgataaa ggagaagtaa aggacgaaga agagttaagg
     6061 gagttgaata ggaaattaat tgatacagcc aaaaaatttg acaaaattcc agtagctact
     6121 ggggatgttc attttttaga caaacatgaa gctgtcttaa gaaaagttct taaatattct
     6181 caaggtttta aagtagatga agaagaaact tatttacatt ttagaacaac agatgaaatg
     6241 ttggaagagt ttagttatct tggagaagaa ttggcatatg aagttgtagt tgaaaatagt
     6301 aatctgattg ccgatatggt tgaagatata aaacctatac cagatgatac ttatcctcca
     6361 attatagagg gctcagatgt ggagttaaga gaaatgtgtt acaaaaaagc caaacgaatc
     6421 tatggagacc caataccaga aatagttcaa aaaagactgg atagagaatt aaattcaata
     6481 ataagcaatg ggtatgcagt tatgtatatt atagctcaaa aactggttgc aaaatctctt
     6541 tcagatggat atttagttgg ttcaagaggc tcagttggct catcttttgc agctactatg
     6601 agtgatataa cagaagttaa tcctcttcca gcacattata tttgtgaaaa cgaagactgt
     6661 aaatattcat tcttttatga aataggtgaa tggggctcag gtgttgattt accagacaag
     6721 gattgtccaa agtgtggaag aaaacttgct aaaaacggtc atgatattcc atttgaggtt
     6781 ttcttaggtt ttgaaggtga taaggaacca gatatagact taaatttctc aggaaattat
     6841 cagccaacaa ttcataaata cacagaagaa ctatttggag aggggtatgt atatagggct
     6901 gggactatag gtactgtagc agaaaaaaca gcttttggat acgcaagaaa atatgtggaa
     6961 gaaaataata taagtgtacc aaatgcagaa gtacttaggc tttcaaatgg atgtactgga
     7021 gtaaaaagaa cctcaggaca acatcctggt ggagttatgg ttataccaga ctataaagat
     7081 gtatacgatt ttacacctat acagtatcca gcaaatgata cttcttgtgg tgttataact
     7141 actcactttg attatcattc aataagtggt agaatactta aacttgatat acttggacac
     7201 gacggtccta ccattataag gatgcttgaa gatatgactg gaataaatat aacagaaatt
     7261 cctttagatg ataaagagac tatgtctcta tttacatcta cagaagcatt aggtgttaca
     7321 ccagaagaaa taaactgtcc tataggttgc cttgctgtac cagagtttgg aactaagttt
     7381 gtacgacaaa tgttacttga cacaaaacct actacatttg cagaacttgt acgtatatct
     7441 ggtctctctc atgggacaga tgtttgggta aataatgcac aagacttagt tagagaagat
     7501 atagttggat taaaagatgt tatatctaca cgagatgaca taatgaacta tttaatattt
     7561 aaaggattac ctcccaaaat gagttttaca ataatggaga gcgtaagaaa aggtaaaggt
     7621 ttaaaaccag aacacattga agaaatgaaa aaaaataatg ttccagaatg gtatataggc
     7681 tcatgtaaaa agataaagta catgttccca aaagcacatg ctgttgctta tgtaatgaca
     7741 tcatttagaa ttgcatattg taaagttcac tatccagaag cattttatgc gacttatttt
     7801 acgacaaaag ttgaggattt tgatgcagat ttaattgtta aaggattaga tgctataaaa
     7861 tcaaaaataa atgaaattga gtcactggga aatgatgcaa ccacaaaaga aaaaggtatg
     7921 ttgaccgttt tagaagttgc tcttgaaatg tatgctagag gaataaaatt attaccagtt
     7981 gatatatata aatcagatgc aactgagttt atagttgtag gagaaaaaac attacttcca
     8041 ccaatggcag caatacaagg tcttggagga aatgcggcta taaacatcca aaatgaaaga
     8101 aaaaatggtg aatttatatc aaaagaagat ttgagaaaga gaacaaaaat atcaaaaacg
     8161 gtaatagaaa ctcttactaa tcatggttca cttgagaata tgagtgagag aaatcaattg
     8221 tcattgtttt aagttagccc aaaaagttgc aaataaaaga attttatgat atcatagtat
     8281 agaaaatagt atactttgca gaaactaaga gtgagacagc tgttctcact cttttatttt
     8341 aaatatatta ttaaaaagct ggtttatttt tattaaatat attttattta agattatata
     8401 aactggtcat ttacaattga ataggaggaa ctttaatgaa aaaaaattta gaagctacaa
     8461 ttgaggagat tgtaactaaa ataacggatg aacatggatt tgaaatggtt gatgttgaat
     8521 atgtaaaaga agcaggagaa tactacttaa gagtatacat agataaagaa gaaggaatat
     8581 ctttaaatga atgtgaatta gttagtagag aattaagtcc aatactagat gaaaaagacc
     8641 ctataaaaga aaattacttt ttagaggtat cttctccagg acttgataga gcattaaaaa
     8701 aagacagaga ttttgttaga tatcaaggta gagatgtaga tttaaaatta tataaaccat
     8761 taaatggatg taagcaattt gaaggtgaac ttgtaggatt aacagaagat aataatataa
     8821 aaatcattgc aaatggtaag gaaatggaat ttaatagaaa agatgttgct attgtcagat
     8881 tagcaataaa gttttagtgg tatattaaat agggaggata taaaaaatga atcatgaatt
     8941 tatggaagca ttagatgaat tagtagttga cagaggtata gataaagaaa tacttataga
     9001 tactatagaa caagctcttt taactgcata caagaaaaac tttggttcag cacaaaatgt
     9061 tagagttgaa tttgatagag acaagggaga tataaaagta ttttctcaaa gagttgttgt
     9121 tgatgaatca gatttatatg atactttctt agaaatagag ttagcagatg ctagagagat
     9181 aaaccctaat tatgaattag gtgatattat agaacatgaa gttacaccta aaaactttgg
     9241 aagaattgca gctcaaacag caaagcaagt agttgttcaa agaataaggg aagctgagag
     9301 agaaatagtg tataatgaat ttatagaaaa agaaaatgaa atagttacag gagaggttgc
     9361 tagagcaaat aaaaatgttg tttatgtaaa tcttggaaga atagaaggta taatgactca
     9421 aagtgaacaa ataccatttg agaattataa agcaggtcaa aaaataaaat tctatatact
     9481 tgaagttaaa aagactaaca aaggacctca aatagttgta tcaagaagtc atccgggtct
     9541 agtaaagaga ttgtttgaag aagaagttcc tgaaatatat gaaggaatag ttcaaataaa
     9601 atctgtagca agagaagcag ggtcaagaac taaaatggca gtaagatcta tagatgaaaa
     9661 aatagaccca attggagctt gtgttggacc aaaaggactt agagtaaaaa atatagtgga
     9721 tgaacttggc gatgagaaaa tagatataat aaaatatagt gaagacccag cagagtttat
     9781 atcagcagca cttagcccat caaaagttgt tagagtagat atagatgaag atgaaaagtc
     9841 tgccttagta atcgttccag attatcagtt atcgcttgct ataggtaaag aaggtcaaaa
     9901 tgcaagacta gcagcaaaat tgactaactg gaaaatagat attaagagcg aatctcaagc
     9961 agaacaagaa atgttaaatc ttgaaaattc caaagaaata gaagctgagg atgttgaagc
    10021 tgaatttatc gaccaagaag agttagatac ttcagaggat aattttagtg aagaagagta
    10081 aggagggatg attttgaaaa aagtaaaaaa agtgcctcaa agaaaatgca tagcatgtca
    10141 agatagagac tctaaaaaag agcttataag aatagttaaa aataaagaag gaaaaatatt
    10201 tttagaccca acaggaagag ccaatggaag aggagcatat gtatgtaaaa gctctgaatg
    10261 tctaaaaaaa gctataaaga gtaaagcatt aaataaagct tttaaaatag atgttcctga
    10321 tgaagtctat gataatctac ttatggagtt agaagaatat gaaaaataat aaagaaaaag
    10381 tactttcgtt cttagggctc gcaactcgtg cagggaaaat agtgtctggt gatgattcaa
    10441 ctttgcttga ccttaaaaaa ggaaaagtaa atttgatttt aatcgcagaa gatgcatcaa
    10501 ataatactaa aaaacttttt aaagataaat caacttttag aaatattcca tatctatttt
    10561 tttcaacaaa agaggaaata ggatttgcaa taggaaaatc tccaagagca gttgtaggaa
    10621 taaaagatga aaatttttct aaaaagataa ttgaactaat agaaatttaa aataaaatag
    10681 ggggtgatct tgtgtcaaaa acaagagtat accaaatagc agaagaatta aatatatcaa
    10741 atgaagagct tataaataag ttagctgaat tagatataaa tgtaactgac aaagattcag
    10801 tattagaagg tgaagagtta gaattagcac ttgaaatgtt aggtgaagat ttatctcaag
    10861 aaaatggaaa tgttatagaa atagatggaa agttgacagt acaagtatta gcaacgaaat
    10921 tagataaatc tccatcagaa ataattatga agcttatgaa aatgggaact atggcaacaa
    10981 taaatcaaga gataagtttt gagattgctg ccttggctgc caaagactat ggctttgaat
    11041 taacagtagc tgaaagtgat gatacagaag ctttagaaat agaagcttta atggaaattg
    11101 aagaagataa agaagaggat ttaaaaccta gaccaccagt tgttacagtt atgggacatg
    11161 ttgaccatgg taagacatct ttactagatg caataagaaa aactgatgtg atttcaggtg
    11221 aagctggagg aataactcag catataggtg cttcagaagt taaaatcaat ggacacaaaa
    11281 tagtattttt agatactcca ggacatgagg ctttcacatc aatgagagca agaggagcac
    11341 aagttacaga tatagcaata ttagtagttg cagcagatga tggtataatg cctcaaacag
    11401 ttgaagctat aaaccatgct aaagcagcag gtgtaccttt aatagttgct attaataaaa
    11461 tagataaacc aggtgcaaac ccagataagg ttaaacagga attagcagac caaggattat
    11521 tagttgaaga ctggggtgga gaagtaatag cagttccagt atcagctaag aaaaaagaag
    11581 gaatagatac acttttagaa atggtattat tggttgctga aatggaagag ttaagagcta
    11641 atccaaataa aagagcagta ggaactgtta tagaagctga gcttgataaa ggaagagggc
    11701 cagttgcaac agtacttgtt caaggtggta ctttaacagt aggtgaccca atcgttgcag
    11761 gtgttgcatg tggtaaagtt agagcaatga taaatgcaaa aggtaagaga gtaaaaacag
    11821 caggaccatc tacagcagtt gagatattag gtctatcaga agttccacaa ggaggagacc
    11881 aatttgtcga agttccaact gataagattg ctagaagtgt agcagcaaga agacaacaaa
    11941 tagttagaga tgaaatgtta aaatcaactc aaagattatc actagatgct ctatttagtc
    12001 aaatgagtga aggttctata aaagacctta atatagttat aaaagcagat gtacaaggtt
    12061 cagtacaggc agttaaacaa tctttagaaa aattatcaaa tgaagaagtt caagttaaag
    12121 taatacatgg tggtgttgga gctgtaacag aatcagacat attacttgca gcagcttcta
    12181 acgcaataat aataggattc aatgtaagac cagttccagg tgcagaatct ctaggagaaa
    12241 aagaaaatgt tgatataaga acatatacta taatatacaa ggcaatagaa gatatacaag
    12301 cggctatgac tggtatgtta gaccctgaat atgttgatga agaaacagga aaagctgaaa
    12361 taagagaaat atataaaata tctggtgttg gaacagtagc tggatgttat gtgactaatg
    12421 gtaagatatt tagaaattgt aaagtaagat tagttagaga tagcataata atacatgaag
    12481 gtgagcttgc agcacttaag agatttaaag atgatgtaaa agaagttaat tctggatacg
    12541 aatgtggtat gagttttgta aattataacg acataaaaga aggcgatata gtagaagctt
    12601 atataactaa agaagtagaa agaaaattat aaattcgtat caaagaaaat cagttagtaa
    12661 agaaggtcga tggtatggca tcatataata gaacaagaag gatagcagaa gaaataagaa
    12721 aagtcgtaag tactatgctt ataaatggag taaaagaccc tagaataaca tctatggtga
    12781 gtgtaactga tgtagaagtt acaaatgacc taagatatgc ttatgtatat gtaagtattc
    12841 taggtggaga tgaagaatcc acactaacag gtcttaaaag tgctggagga tatataagaa
    12901 gagaagtagg aaaaaatata aaacttagat atattcctga gatagtattt aaacttgatg
    12961 attcaataga aaaaggaatg tatatggata gccttataaa aagagttaat gaaaaaaatg
    13021 ctcaacaaaa tgaggatgaa aattatgatg aataatatgg atagtattat aaactatatt
    13081 tatgagggtg actattttat agttacctct catatcagtc cagatggaga caatattggt
    13141 tctacacttt caatgtatta tacattgaag aatttaggaa aaaatgtata ttatgtactt
    13201 gatgatgaag ctccattaaa tttaaggttt ttggttgaag gtgtaaaaat ctataagtct
    13261 aatgaattta atatgaagaa ctattctcta attgcacttg attgtgggga taagatgaga
    13321 atatgtgtga gtgatgaaat caaaaataat gcatcaaaat taatctgtat tgaccatcat
    13381 gctagtaatg attcatatgg agatttaaat tatgtagata ttgatgcatc ttctacatgt
    13441 gaattagttt ataatttatt agttagattt cagcaaataa aagatattaa tattataaat
    13501 gaagatatag caacatgttt atatacaggt cttgtaactg atactggaaa cttttcgtat
    13561 tcaaatgtac atgcaagtag ctttgaaatg gcaaaaaatt tacttgtttt aggtgcacaa
    13621 aaaaatacta taatacaaaa tatatatcaa agcaattctt ctggttatta caaattatta
    13681 ggagaagctc taaagggttt ggagatattt gattaaaaaa tagttacgca cactcgtgac
    13741 tttagtcatg agttagtagc taaaatagtc agcatatagg gaaacttata tgtagagata
    13801 ggata
//
LOCUS       DAENCV010000097        13769 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAENCV010000097 DAENCV010000000
VERSION     DAENCV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.098"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            389..1039
                     /locus_tag="KQD82_002835"
     CDS             389..1039
                     /locus_tag="KQD82_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG4381294.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1253..1924
                     /locus_tag="KQD82_002836"
     CDS             1253..1924
                     /locus_tag="KQD82_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381295.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1921..3051
                     /locus_tag="KQD82_002837"
     CDS             1921..3051
                     /locus_tag="KQD82_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381296.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKIRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFKIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3466..4311
                     /locus_tag="KQD82_002838"
     CDS             3466..4311
                     /locus_tag="KQD82_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381297.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4311..5339
                     /locus_tag="KQD82_002839"
     CDS             4311..5339
                     /locus_tag="KQD82_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381298.1"
                     /translation="MYSKFFTLVKKEFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5342..6355
                     /locus_tag="KQD82_002840"
     CDS             5342..6355
                     /locus_tag="KQD82_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381299.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6883..8133)
                     /gene="cme"
                     /locus_tag="KQD82_002841"
     CDS             complement(6883..8133)
                     /gene="cme"
                     /locus_tag="KQD82_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG4381300.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8219..8965)
                     /locus_tag="KQD82_002842"
     CDS             complement(8219..8965)
                     /locus_tag="KQD82_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381301.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9118..10140)
                     /locus_tag="KQD82_002843"
     CDS             complement(9118..10140)
                     /locus_tag="KQD82_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG4381302.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10140..12560)
                     /locus_tag="KQD82_002844"
     CDS             complement(10140..12560)
                     /locus_tag="KQD82_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG4381303.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSNKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLSAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPKSLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVYFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKALKENP
                     KSITGRYL"
     gene            complement(12646..13566)
                     /locus_tag="KQD82_002845"
     CDS             complement(12646..13566)
                     /locus_tag="KQD82_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4381304.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4843 a         1804 c         2168 g         4954 t
ORIGIN      
        1 gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt taggacttga
       61 aattgtctaa aaataaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata
      121 gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt
      181 atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg
      241 taaatagaat tacatattct gaagaacggt tgcctaaaca gtatatagtt tttcagaaat
      301 atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta taaattctaa
      361 agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt
      421 attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt
      481 aatagttaaa ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca
      541 gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg atactaaaga
      601 attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgtct
      661 tatagttttg gttcaaatgc cagtagtata tgctttatat catgtatgtt tagatatgac
      721 aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat ttgataattt
      781 gtttatatta ccttgtatat atacattaat tatgttagct tcaaatttag tgaattatat
      841 accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac
      901 cataacaagt cttatactaa ctgttagaac tccagtagcc ataggtctgt actttataac
      961 aagtgcaatt tattcattaa ttgaagatat ttgttttaga atatatttta atcgaaaaaa
     1021 tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc taacatagaa
     1081 aaagttagta ggctattttt atctatgata tcaattccat taagaatagt ctaaaaaata
     1141 ctatcaaaat gctataaaaa atactatcaa aatactataa aaaaatagtt gaaaaataaa
     1201 taaattaata taaactaaaa taaagcctac aaaaaagagg tgataaatag aaatggctaa
     1261 aatactaatt gtagaagatg aaaaaaggat gcaaagtatt attgtagaat atatgcaaaa
     1321 gggaggatat acttgtataa ctgctgatga tggagtcgag gcacttacaa tattaaagag
     1381 caacaatata gatttaatga tattggatat tatgatgccg tatttagatg gattttcagt
     1441 gtgtagagtt tcacgtgaaa tgactaatat acctattatt atattgacag ctaaaggtga
     1501 agaggaggac aaattaaagg gttatgaata tggcgcagat gattatataa caaaaccatt
     1561 tagccccaaa gtacttttag caaaagttaa tgccttgtta cgtagataca ctactgatat
     1621 tcccaaaaat tcattgagtt taggaaagat ttttattatg gtagcatcaa ggcaagtgta
     1681 tgtagaagat aaattgattg atttaacata taaagaattt gaattattgc gtttatttat
     1741 ggaaaaccct aatcaagttt tttccagaga gaaactttta aattgtatat ggggatatga
     1801 ttttgaagga aacacacgaa ctgtagatac acatataaaa accttacgca aaaagcttgg
     1861 tagtgaagga caccatatag ttacattaat tcgttcagga tataagtttg aggttaagga
     1921 atgaatagtt taagaaagat tatagaaaaa ctgaaaaatg tagatatttc aaattggatt
     1981 aaacaaaatt ttggactata ttcagttaga aaaaaagtgt ttttactttc taaattatca
     2041 ggagcgttaa ttatattatt ttatctggtt gttgaagaat tttcgataaa ctctaaattt
     2101 gaattttgga tttggtttat aatactggtt atatttatta tgagtattga tttcttattg
     2161 ggaaaattta tttcagaacc aatcacatct ataaataaat ctgcaaaaag tatgtcccaa
     2221 cttgattttt ctaatccatg tactgtaaat actaatgatg aatttggaga actttctagg
     2281 agcctgaata caatgtctac aaatctacaa caggcattat cagacctaga aagtgcaaat
     2341 attcaacttg aaaaggatgt aaataaggaa agaatgctat tggaacaaag aaaggagttg
     2401 gtagatacaa tttcacatga aatgaagact ccacttggca ttatacgtgc gtatacagag
     2461 gggcttatgg atgaagttga tgaagaaaaa aagaaaaatt atatgaatgt tataatagaa
     2521 gaaactgata gaatgaacaa tatgattgta tcattattag acttatctgc attggaagca
     2581 ggagtttcta aacttaaccc agagcgtttt gattttatag agtttgttga aactgtggca
     2641 ggtcgtcttt taattgatat accagatgcc aatttttatt ttacatatga tttgccagag
     2701 tatgaagttt ttgtagtggc agataaaata agaatggaac aagtagtaga gaatctaatt
     2761 attaatgcaa agaagcatgt tgtatataat ggtaatttag atttatcgat tacatgtgaa
     2821 aatggcttgc ttttatttaa aatttataat gatggtagga ttatagagca agatgagatt
     2881 tcaaagattt ggtctaagtt ttatcgtagt gttcaatctc agcgtacagg aggttcaggt
     2941 ctagggcttg caattgtttc acaaatactt acaatgcaag ggttagaata tggagtgaat
     3001 aatcgtgata gaggagtaga gttttatttt atgattccaa ttgatgaata atttagtatt
     3061 aatttataaa ttataagtat ctgatgtaaa atatttttct ttgtagacaa tagatatcta
     3121 aagagaataa aataaaaaag tctaataaaa aaatttaata aaaagcttaa tgagaagtct
     3181 tgttttcctg atgaaaacaa gattttttat taagcttttt attatttcac ttcgatttca
     3241 caagagagac actttgattt caccaaatag aaataaaata agttaaataa attaaaaaaa
     3301 tataagaagt aattaaatac gcttacaagg gggggcactt atgaaatggt atacatggtt
     3361 gactattata ttgataatat tggtttctat tccgcttaaa attaaattta taaaatggtg
     3421 ggatagacga caaaaagaaa aaaataatga aggaggctat gatagatgat aaaagtagaa
     3481 gagttgaaat tttcttatgg aaaagataaa cagatattgc atggacttaa ttttgaagta
     3541 aaagaaggag aaatatttgg atttcttgga cctaatggtt caggaaaatc cacaacacaa
     3601 aaaatcttaa atggtgtttt aaaaggttac aatggtcatg tatccatttt tggtaaggaa
     3661 gttaaagcat acacagaatc tttatatcaa aagattggtg ttttatttga atttccttat
     3721 ttatatacca atttaagtgc aattgataat ttagaatatt tttcgtcttt ttatcctaaa
     3781 aatcaaagga gagatatata tgaactctta gatttgttag agtttaagaa agagtttata
     3841 aataagcctg tatcatccta ctcaaaaggt atgaagcaac gtattagtat ggcaagagca
     3901 ttggtaagta atccaaagtt gttattttta gatgagccta ctagtggact tgacccatct
     3961 ggagctgtac tatttcgtaa gattattgaa gaagaacgaa aaaaaggaac aactatcttt
     4021 ttgactactc ataatatgct agatgcagat ttaatgtgta acaatgtggc ttttattgca
     4081 gatggaaaga ttatggtaat agataaaccc aaaaatttaa aaatgaaaaa tagcaataat
     4141 aaagttgaag tggaatttgt atataatgga aatcgagata ttgaaattgt agatatacaa
     4201 gagttagaat ctggaataac ttttaaatat gatgaaatct taagtgtaca ttcaaaagaa
     4261 cctacactag aagaagtttt tattaaatac acaggaagga tgttagtgta atgtactcta
     4321 aattttttac cttagtaaaa aaagagtttt ggatgatgat ttatggtaaa ttttttgtgc
     4381 tgacacttgg ctctctcata ctttattcgt gttatattaa ttttggttat gtaaagttta
     4441 tggatgaaga tagttatcac aataatgtat atctatataa tccagagaat ataagcatga
     4501 gtgcatctcc attaattaat caagtttctt ctataggtga attaaaagca aaacttgatg
     4561 aagatacaag tgcagtttgt attgatatga gttccagtaa gccgaaagta attttacatg
     4621 aaagtattaa aggagttgac gattatagag ctgactatgc attgtcatta ttatcatcag
     4681 aaaaagacca taatagcaaa atatcagaca acaatgcaat aattcaaatt aatgataagg
     4741 atacatctga gataaaacaa agaaaagaca tgacttgtga gattttattc tttgaaattg
     4801 tagctattgg atttttaggg attgcctctt tattatttaa agaaaagcag atgggagtaa
     4861 tgcgtattac tggtatttta cctgtaaaaa gaagtctttt tatattatca aagatatttg
     4921 cttttttgat atgtgaccta tgttttacag ttcttttagt agtatttaat attggatttt
     4981 ttgaaggaat aaaaatactt ccacaagtat tagttcaagc tagtatttta tcaattatta
     5041 tgtctttgat tggaatagga tgctcaatgt tattgagaga ttttaagcaa ttttctctta
     5101 tatatcttgt ggttgctgta ttcataacta cacctatatt tctatctgca aatacatctt
     5161 ttgaaataga ttggattaaa tatcatccct tctatcatgt ttatagtcag cttaaaaatg
     5221 cgtttttcat gaatttttca aaagaattag tttattttgt agtgtgttct ttgacaataa
     5281 tagttctatt tttacttgta aaatatattt ttaataaaga gttggttagg gagggataat
     5341 cttgatagga ttacgttacc agttaaagag tataaaaaaa gataaaatgt gtataatttc
     5401 cttcttttta ccagttgtta tggcgatttt aataaatttt atgggaacaa ttgatatatc
     5461 ttcattaggg gagatacgtt ttggtatagt gaaggataca cttcctgtac aaacagagca
     5521 gtggttgaag aagtatggta tggtaagtgt atatgacaca aaaatgcatt taatacgtga
     5581 aattaaggat attaaaacgg acacaattgg agttgttaga gatggtaaag ggataaaaac
     5641 tgtgctttat ggaaatgaga ttaatgctat aaaaaatgtt ggaaagacat tgccagagat
     5701 gtttagacta aagggtgatt taaaagatgt tgaggttaca atattgccta aagatagtat
     5761 tttaaaagag tttcaaaatg tttttattgc catgatttta attactgcta tgtttatggg
     5821 atgcaccttt aatgcaatga atattatatc tgaaaaagaa gatggaatat catttataaa
     5881 tgaagttttg ccacagagta gaagtcagta tattatacaa aaagtatttt tgggtctttt
     5941 atgtgggtgt ttatcttcaa tgataactgt atttatatgt ttggatattt caatattaga
     6001 tatgataatg atgttgttac tcgttgtatt ttcagcattt atttcatcac ttataggttt
     6061 atttattgga aagttgtcag aaggtcttat ggtaggaatt gtttatatta aaatagtaat
     6121 gattttattt ataggtactc ctttgttaat atatcttcta ggggttaata ccagtggtat
     6181 agggaattta tgctatataa tgccttcagt tgctacattt aatggtatta tggagattat
     6241 taatggtaat gtcattatta tcaaagatat tgtaattttg gctgtacatt gtgtagtatg
     6301 gtttatgata tatattattt tagataaaaa aagaaagtta tcttttagta gataactcag
     6361 tataattata attgggacaa tttaataggt gatttaatat actttgtata gtgtaattta
     6421 tgtagtgcaa tttgtatact ttaatttatg tagtgtaata gttagtttta ttttataatt
     6481 attcaatata atttagtaag aagttcagta caattagagc tacttagtgg aaaacttaat
     6541 aggtacttaa actatataaa tactttattt tcctccattt gtactataaa tttaatttat
     6601 gatataaatg gaggtattat tatatgattt gataaatttc atcattataa cctacaagta
     6661 aaggtattat tatataaagt taataaagat agtatcaaaa tattatttgc tataaagaga
     6721 gggactcaaa ataaagataa acctattttt caatgttgaa atgataaaat atatttaatt
     6781 tcatataaaa aattttcttc ataaattaaa aagtccattt taagcccccc tcagcccccc
     6841 tcatttttaa ccagataaat cgagttaaac tatttaaata tattactcgg attcatttga
     6901 cttagatatt ccttgcttgt aaaattgttt tgaatagggg atagccaaag caaaaaataa
     6961 aagtataatc cctgctatga ttattaaatt attaatagaa attacatctg ccattggtcc
     7021 gaaaatcatc ataccaagag gaataaaacc agtatatata ataataggta agctaagaac
     7081 tcgacctagc atttgtggtg ctacatgctc ttgcaacata gtcatagctg atgcttgaat
     7141 aattggaatt gaaaaattcg taaaaaacat aaatataaaa aattgccaag cttgtgttac
     7201 aagacctaga ataattgaaa aaatagcata ggataatatt gctagaaaaa aggtcttatt
     7261 tctattttta aaaccaccaa aaactcctat aatcaatcca cctaaaacca tacctaaaaa
     7321 tccaatcatt tcaataatag acaggtatat ataacccttg ccaaatgttc gttcaataag
     7381 aagtcctata agaaatcctg atggaacaca aagaagcatg aatgctccat aagtaaaaag
     7441 cacttttcta atgaacttat ctgaaaaaca gtactttatc cctgatacca tatctgcaaa
     7501 aaatgagact ttttcagttt gttttggtat atcatgttta ggaattttga tgaatatcaa
     7561 taatacaata gcaataattg cagtaaatac atcaattaaa agaacttgat ttattttatt
     7621 accaaatcca agtattgcag cagctacaat aggaactata aattgtacta tagattgtag
     7681 gctactattt ataccattaa aacgcattaa atgttcttct ggtacaagtt gaggaatcat
     7741 tgcgtttaca gcaggtgttt gtacaccact tcctagtgaa cgtataacag atattactga
     7801 tatggctatt aattcacttg ttgtgccatt tgatgacatc ataaatagaa ttagaactaa
     7861 tgtagatata gcaattgttg aatctgatac aattatgagt acctttcgat tgtagtagtc
     7921 tgcccataca ccagcaaaaa aagaaattat catttgaggt ataaaggagc atacagttaa
     7981 aactgttacc catacacctg atgatgtctg aagtgtgaca taccatatca ttgccatttg
     8041 tacaagtgat gagccaaata aggaaatata ttgacttgct aaaaaaagaa tgactttttt
     8101 cttccatgta gaattagtat ttttgttttc catcctattc ctcctttgat ttgtgtgttg
     8161 aatttttctt gctcttaagg ataacacaca aatcaaagtc tggagttaga gtggagggct
     8221 aggaaaattg ttttttcata atttgaagat gagatttcat ggtttttgca ttatgttcag
     8281 cttctttcaa tgaagttatc agtttgtcac acactgagat aatttcgtca tcatcttttg
     8341 gagtgtcaag gacagtttta atattagtat taggattaga agataaagca tttaacattc
     8401 gtaaaatagc tgaaagagaa taatttgcac aacgaagtgc gcttataatt ttaagtcgtt
     8461 ttatatcttc atcagtatat acacgataac cattttgttt tctttttact tttaacaaac
     8521 cattcatttc ccaatttctt aaagtatcta ttgtaacatt taagtgttct gcagtttctt
     8581 ttctagttaa aaagagttta tttgtttctg gagtatttcc atttaaaatc tgtgcaacaa
     8641 tagtaagtgc ttcctcggca ttagattttt ctatttctaa ttgttgcaag tattgattta
     8701 caagactaat agccttatca aagtccctat tcgctgatgt tcttacaatc tcaagggcat
     8761 cttttcttaa tcccctttgt accacttcaa ttttcaaggc tgtacgagca aatctaaatt
     8821 gttctatatg aaaatcatta aagatacgat aaccattggt attacgctta ggtttaggta
     8881 ttaagtttag ttcttcatat aaacgtactg tattaggatg tacaccaatt aaatctgcta
     8941 cctctgatgt cgtgtaagtg tccattagta ttcaactcct tttagatata tagtattagt
     9001 cataataaca ccaaacaaaa gtctggtgtt ataatggagg gtttggctat ctaagagtaa
     9061 ttagtttatg aaagagtaat tagtttatga aagagtaatt agtttatgaa agagtaatta
     9121 atctattata gaagtaagta tatatttttc aataaactcc catctatcac gaggtttaaa
     9181 cactatttta gatggtatgg ctattactaa gtcatattgt ggaatacaac aaatagcatt
     9241 tcctccagtt cccattgctg aataccacaa aacaccatct tttttacata gccaccacag
     9301 ataaccgtat tcattactat tcattttaac tgattcttca atccacgatt tagatataat
     9361 acgtttatta ttccaaatac catcatttaa atacagaaaa ccaaatcgca acatatctct
     9421 agcactcaat gtaagtcccc agccacctat agaataaccc tgtctatctt ttgcccaacc
     9481 cttaagattt tttccaaaga aatcttcaaa accaaaatct ttcatatcat aatctggtat
     9541 aactttcata ccaataggtt taaataagta ttcattagca aattcacgag cagattttcc
     9601 agtagttttt gttataatag ctgaaagtag gtgcgttcca gctgaagaat atttgaaagt
     9661 accaatttcg cctttttgac caagttgatt caaggtatat tttatccaat ctttttgttt
     9721 gcaaagtctt tctaaagact cagtcatatt tttaaaaggg tatggtgcag tcattgtaag
     9781 taggtgacgt atagttactg cttttttaca tatttcagaa ggactacaag tatattcagg
     9841 aaaaaaatca acaacctttt ggtcaacatc ttttatatat cctttatcta ttgcaatacc
     9901 tattaaggct gatataatac tttttgtaac tgatgcaaca tgaaaagtat catcagcatt
     9961 atatccatta tagtactttt caaagacaat ttttcctttt cttgcaataa ttataccatt
    10021 tatattgcta tattgtgaga ttattaaagg ttcaagttgt gaaagctttt ctgaactcat
    10081 accaacactt gaaggatcta ctttttcaaa aattaagtct aagcaattgt ttttattcat
    10141 taaagatacc tccctgttat agattttgga ttttctttta gagcttttgg agaaccttct
    10201 gcaattatat aacctccttt gtcaccacct tctggaccaa gctcaacaat ccagtcacaa
    10261 acatttaata catctatatc atgttcaaca acaattacag aatttccatt ttcaaccaat
    10321 tcatctaata attgaatcaa cttagaagta tcatatagac ttagaccagt agttggctca
    10381 tccaatacat aaaggatatt accatttcgt tttttaccaa tttcttttgc aagtttgatt
    10441 ctttgggatt caccaccact taaagttggt gtaggttggc ctaattttat ataccccata
    10501 cctatttgtt ctaatacatt taagtgtgaa acaatatttg gaatatcctt gaagaaatat
    10561 acagattcag aaacactcat atttaaaata tcatgtatat ttttattttt atatttaata
    10621 gataaagatt catcattaaa tctttttccc tgacattcac tgcatatatg gtctatactt
    10681 agattatttc ccaaccagac cttttcatat ccactaccac tacatttagg acaagcacct
    10741 ttggagttaa atgagaaatg accagctgta aatcctcgtt ctaacgattt tggctgacct
    10801 gcaaatagtg tacgtatctt atcccatata ccaatataag aagcaggagt tgagttcata
    10861 tttttgccaa taggagattg ggatatctct gaatatccag aaatatgttt agtaccaatc
    10921 agtctatctg caatagtttc aacaactgca taatcgtctt cctcacctat ttcactctct
    10981 gaagaattta tttgattatt gatagtatgg ttattaaatt ggcttctaag aagaggaagt
    11041 agggtgtctg atataagtga actcttgcca ctacctgatt ttccagatat tccaaccata
    11101 gcactcaaag gtaatgatac agttacattt ttaagattat ttgtatttgc attttgaata
    11161 gttatacaag gtattttatc agtatgtgta atatttttat tgcttgttct attaggcatt
    11221 ggatattttc cagatagata ctgcccagta agtgatttgt tggattgaag tagaccttct
    11281 aaatctcctt gatatactaa ttgaccacct tcaacaccag cttttggacc aatatcaata
    11341 atatgttctg ccattttaat tgtgttttta tcatgctcaa ctactatgac cgtattgcct
    11401 aaatctttaa gtttctttat tgattttaag atgtctattt tttctgattc atgcaatcca
    11461 gctgttggtt catcaagcac ataaattaat gagtccatct ctgaatcgag atgtgaattt
    11521 aaaaatagtc tttgcatttc cccaccactc aaagttgaca tttctcggta taaggataga
    11581 tgacctaatc gtgattctat taaatgttga gttttggata aaatatcatt tacaagattt
    11641 gtaccaaatg tgtttaaatt accttgattt attaagagtt taaggaaatc atcaatttct
    11701 aaaatcgtca tttttccaag ttctccaata tgttttccgt ttaaaagtac tctacgtgct
    11761 tcttcaccaa ttttaaaacc attacaatct gaacaggcag tcatagtata aactccattt
    11821 aaatcttcgc cccttttgta acgattgtga aatacctttg ataaagagga acttttttta
    11881 ccattttcat aatgaccata gagtacttca tttcttattt cttcaggtag ttgtaagaat
    11941 tgtatgtcta aataatcgtt gaatttttta gaaaatactc tcaaatatcc tggtgtaatc
    12001 tttgctctat ctaagacttg ttttagggtg tcatgcttat caaggactaa tttttcaata
    12061 ttaatctcaa aatatgcacc atgtccagag cacttcatac acattccatc tgaagagttg
    12121 tatgagaaat aacttggtct taatcgttct tcctcatatc cacaattatt gcaaattaga
    12181 ttatcgctta caggagtatc acacatagta cacattattt gaccttccac tgaatacaat
    12241 agtgtaagca tgttgagaat attggttctt gtgcctactg ttgaacgagg attgctctgg
    12301 cgaataatat tttgttgaac agctatagtg ggagctaaac cttgaatatt atcaaactta
    12361 tcttcactat caatacctgc aagtatacct aaagattgca gatattgttt acgcccttcc
    12421 tcaaatacaa tgtcaaacat aaggctagac ttacctgagc cactgacacc tgttgcaaca
    12481 acaagcttat ctttggggat agaaacatct atatttttaa gattgtgtat ctttgctcca
    12541 ttaattttta tttctttcat tggttcacct cattttatca attttatcaa accgtaaaat
    12601 agggatatat taatccttaa tacaagaata gattaggtga ggttattaaa aaggttctat
    12661 aggtaaatat atatcgacca agtgtctatt ttgagggtga ttgtgtgggt tgttaagata
    12721 tacttcaaaa gggaaggagg cacgtagctt ataattacca tttgatagcc attcattata
    12781 cataaaatcc cacgcatcac tatattcaga ttttgaaata tcaaaatgtc ccacaagata
    12841 ttttccagaa ggtattatca tattgccaag attgtcattc tctttgacaa ctatgttatc
    12901 tggaatagtc atgcaaatac tagtccttaa ttgatgctcc tgtgtaaatt gtggattatc
    12961 atgataaatg gctaatattt gtgtgttttc aagtccaagt agattctgct cacttgcata
    13021 tgtgaataac cgttctaaaa gttttgggaa agtactggtt agattaccat atgaaccagt
    13081 atacctagtg taaatagtat tcatattatc aagttctaga atttcaattt cacctttaac
    13141 ctctctagta ttcttacatt taggttttga tatgcttcta ttgtactgag aaattttata
    13201 tggttctttg caattcttgc tataattgtt tctatatttt atagggctta ttttatagta
    13261 atttttaaat gctctcgaga aaactgctga atcagtaaag ccaaagtagt gtgcaatatc
    13321 agtaacagtc atgtcagttc tatgaataag aaaagcagtt gcattttcta gttttagcct
    13381 atttacatac tgtgccaaag tttctttttc tattgctttg aagattctat gaaaatgaaa
    13441 ttttgaaaaa cctgctatat tagaaagttc atcaagagaa agaggttcat gtaaatgttc
    13501 ttctatatag tcttgcactt tgcaaatacg atttagatat tcttttttac ttttagcatt
    13561 tgtcatatat agccctcctt attgtgtaaa atgtgtatca attaatttat agcaatatag
    13621 taataaaatt ttatgttttt gtaatttatg ataaataaga tagcgtttaa ttttttataa
    13681 ttgataaaag atataagatg ccttatattt gtctttgcat ataagtatag tcttcgtaat
    13741 aattagtata ccatagtgat ttattttta
//
LOCUS       DAENCV010000098        13746 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAENCV010000098 DAENCV010000000
VERSION     DAENCV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.099"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..302)
                     /locus_tag="KQD82_002846"
     CDS             complement(<1..302)
                     /locus_tag="KQD82_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG4381305.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFE"
     gene            complement(525..1469)
                     /locus_tag="KQD82_002847"
     CDS             complement(525..1469)
                     /locus_tag="KQD82_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG4381306.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            1855..2736
                     /locus_tag="KQD82_002848"
     CDS             1855..2736
                     /locus_tag="KQD82_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG4381307.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(2797..4005)
                     /locus_tag="KQD82_002849"
     CDS             complement(2797..4005)
                     /locus_tag="KQD82_002849"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG4381308.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(4078..4330)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4482..5471)
                     /locus_tag="KQD82_002850"
     CDS             complement(4482..5471)
                     /locus_tag="KQD82_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG4381309.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            5623..6063
                     /locus_tag="KQD82_002851"
     CDS             5623..6063
                     /locus_tag="KQD82_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381310.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            6390..6617
                     /locus_tag="KQD82_002852"
     CDS             6390..6617
                     /locus_tag="KQD82_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG4381311.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            6643..8763
                     /gene="feoB"
                     /locus_tag="KQD82_002853"
     CDS             6643..8763
                     /gene="feoB"
                     /locus_tag="KQD82_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG4381312.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(8896..9714)
                     /locus_tag="KQD82_002854"
     CDS             complement(8896..9714)
                     /locus_tag="KQD82_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381313.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            9921..10205
                     /locus_tag="KQD82_002855"
     CDS             9921..10205
                     /locus_tag="KQD82_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381314.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   10373..10864
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10440..10468
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(11523..13616)
                     /locus_tag="KQD82_002856"
     CDS             complement(11523..13616)
                     /locus_tag="KQD82_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4381315.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         4850 a         2085 c         1613 g         5198 t
ORIGIN      
        1 tcttcaaaag ctattctctt atacgcttca gctacttctg gataaccttc tctatcagct
       61 tgtctactca ttgctaaata cattcctact tctgtacact caccagtaaa gttagctctt
      121 aatccttcga ttatttctgc atctacacct tgagctactc ctattctatg ttcatcagcc
      181 caaaccattt cgcctttcat ttcttcaaat ttatcagctc caactttaca tactggacat
      241 tgtgctggtg cagcatctcc ttcatgtata tatccacata ctgtacaaac aaattttttc
      301 ataattaatt tcctcccaat tttatataat taaattttta tttttaagtt taataacttg
      361 ctacaagaat atatataccc cagactttat agcttaaaca ctaaaaataa aatttatttc
      421 ctaattaatt ttatcacttt gaaacaccag tatcaaccaa gttttgatgt caaatttcta
      481 cataatacat aatttatttg attaattagg aaatagttgt catattaaat actttctaat
      541 gaagactcct ctgaatcttt aagtttcact tttttgctct ttaaaaaaga taatttagtt
      601 tctgcagtta tgattgcaac aaatatcaag atactaccaa taaccaattt tgctgtaata
      661 agctccttta aaattattat agaaaatatt gtaccaaata cagcctctgt ggataatatt
      721 atagctgttc tagttccatc aactcttttt tgacatatag tctgaaataa aaatccaatt
      781 gtagtactaa atacagctag atacatagtt cccatgtaac cagataattc agcttccatc
      841 tttaattgac ctgcaaatgt ttgaactaca acagacatta aaaacgctac tgtaaattga
      901 atagctgtca atgccattgg gttgttatct tttgcaaact cacttgtaaa gaatatatga
      961 aatgcaaatc cgaatgaaca tataagtgtt agaaaatctc caaaattaat agagaaatca
     1021 gcttctaaag aaagtatccc aattcctata agagctacca aactacttat tattcctatt
     1081 ttatcaagtt ttcttctata aagtataaat cctatgaacg gaactattac aacattagct
     1141 gctgttataa acgcattttt agatggtgta gtgtacataa gtccaatagt ttgtactgtg
     1201 aatgctagaa ataaaaatat tcctaaaatc cctcctgctt ttaataattt tttacccata
     1261 ttggcacgaa tctgtttaaa aaacagtaaa ttcataatta tagctgccaa gaaaaatctt
     1321 aaagttatga tttgtagagg tgtaagccca ccgtctaatg caaattgggt tgctacaaag
     1381 ccacttcccc atataattgc tataaatatt agtcctattt ctccaaagta ttttttcaat
     1441 aaagccccct cctttttatt tttttgcaaa attataaacc ccctaaacag caagagagtt
     1501 tttctataag cattactata aattaatata gtaaattttt tcagtatttt aaatctaaat
     1561 ttacatacat tttgagctca aatttatatc cactatttaa cagtaatata ttaatcattt
     1621 tttgtaaaca cctttttaat aaatttttat attttttata tttttttacc tatactaaat
     1681 ttttttatag atatttttaa tatatatgat actttaatat tatgcaaata atgtcatata
     1741 agttacactt tagttatctt agttacaact ttgtcggttt actttttcta ctttcttcga
     1801 tataattata atatattttt gatacaattc gacaaaaggg ggaatgctgt aattatggta
     1861 aaaaaaagaa aaaagaaaac tgttacactt actatacttg ttgtaatagt tattggtatt
     1921 ttttctatgg catgggcgga catgtctaga aaaaaagaaa tgaaagaaac tcaagaaaga
     1981 cttaaagctg aaagattaga aaaagaaaga attgaaaaag aaaaaaatga acctattatt
     2041 ggtgcaaaaa aagaagctgt aaaagaatat ggatatgacg caaaaatagt ttgggacaaa
     2101 cttaacaaat acgactattc aaacaatgga gaaaagatag tctttcttac atttgatgat
     2161 ggaccatcta ctactaatac tccacaagtt ctagatatct tgaaaagaca tggtgttaga
     2221 ggaactttct ttattaaggg tgattctctt gaaagaaaag gagctaatga aattttaaaa
     2281 agaacatttg acgaaggaaa tgcaatagcc catcactctt atactcatga ctataaaaaa
     2341 ctatatccta acagaagcct aaatctcgat acattcgtaa atgaacttaa caaaactgat
     2401 gaagctatga aaaaagtctt aggaaagaat ttttcctcaa atgtagttag atgcccaggc
     2461 gggtatatgt catggaaaaa tatggaacct ctaggaaact acttaaaaga gaaaaacatg
     2521 gcatcaattg attggaacgc tttaaatgct gatgcagaag gtaaaaagaa aaatgcacaa
     2581 gaattatttg aacatgcaaa gaaatcttct gaaggcaaag aaatggttgt tcttttaatg
     2641 catgatactt atggaaaaca agaaacagta aatgcattgg accaaattat aacatatttt
     2701 aaagacaatg gttatcaatt taaaatttta atataataaa aaagcaagtt tattgaatgt
     2761 acattttata ctcaatagac ttgcttttac tttactctat tttactagct cgatttttac
     2821 aaattttttc tttcctattt gaacaactaa ttcactctct tttacaatag tgcttaaatc
     2881 ctctactttt tcaccattta cctttactcc accttgagaa gctaatcttc taacttcact
     2941 cttactctta acaatctcat tagaaactag tacttcgatt aagtcaaatc catcttcctt
     3001 aacttgaatt gtttgtatat cttcaggtat ttgacctttt tggaatacag atttaaatct
     3061 ttcttcagct tctttagcag attcttctcc atggtataat gttactattt ctcttgctaa
     3121 gttcatcttt atatctcttg gatttactga accctctttc aactgagatt ctattttatt
     3181 aacttcatca ggatgaacat ctgtcactaa attgtagtat ttaattataa gttcatcagg
     3241 aatttccata gatttttggt acattattcc cgcttcttca tctattccaa tatagttacc
     3301 taaactctta ctcatttttt ctttaccatc taatccttct attaaaggca tcattattac
     3361 tatttgagac tccataccaa attctctttg taatgatctt cccattagta aattgaatct
     3421 ttggtcagtt ccaccaagtt ctatatcagc ttctaatgct atagagtcat atgcttgcat
     3481 taatgggtag aagaactcat gtactgatat tggcatttgt ccttcatatc tctttttaaa
     3541 atcttctctt tctaacattc tcgcaacagt aatagttgct gctaatttta ttacatcttc
     3601 aaaatttaac ttagctaacc actcgctatt aaatcttaca atagtttttt ctttatctaa
     3661 aactttgaaa atttgttctt cataagtttt tgcatttgca agaacttgct ctgttgttaa
     3721 agcttttcta gctttagatt ttccagttgg gtctccaatt tttcctgtaa aatctccaat
     3781 tattattact atttgatgac ctaaatcttg taattgtttc atctttctaa gaactactgt
     3841 atgccctaaa tgtatatctg gagcacttgg gtctagacct aattttacta tcataggctt
     3901 tccttctttt tctgatttaa taagcttttc ccttaactct ttctcatcta ttaaatcatc
     3961 tacacctttc ataattattc gcatttgctc gtctatcgat ttcatttttc attcctccta
     4021 aaatatttat ataatctata atttttattt ttataataaa aaaagctttc atcctcttaa
     4081 aaggacgaaa gcattgctta cgtgttacca ccttagttca tcaatatctc acgatattaa
     4141 tctcttagag tacaattaca tatactcgtg tactataacg tgtactaatc cgatatctct
     4201 taatggttgt tatctttcag agatagtgct ctgagatgta ttcaaactaa atcaatttat
     4261 tccatttcac caaactggaa ctctctgtta aataaaatct agccctactt cttctcttca
     4321 tagcatttat atattcagat tatttcatca attacaagaa atttttctta acttactcat
     4381 aatatattat tatttttata ttttgtcaac cttttttatt attatataca tgttttttta
     4441 cattatacta cttttttata ttttttacca atacccaaaa tttatttttc aatcaaaaga
     4501 tttatttgtt tattgactat attactctga ctcaatctta tatttatttt cttatttaaa
     4561 aatgcgttta taattattaa actaataact gttacatcaa atataaaaaa cagcaataaa
     4621 attattatca aaaataaaat tctagcatat aataattttc taaacttagc tattataaag
     4681 ttttgtagtg acatacatga gtctctaatg ataaaataga cacttatata ggttattgat
     4741 ataaagattg tttttaaata aacagaatca actttaacat tcaacagttt tagaacttca
     4801 aaaatggcta gataagaaac acatatgtaa aaagcttttt ttgagcaaca taaaaattgg
     4861 tttaaatttc taaaattctt aaatattaaa aattttttta aagaattaga atctaaaata
     4921 tgcaatttat tccctaaaat taaacttaat aaatatatgc aaaacaccat accaaaagga
     4981 aacaatgcta tcattaaata ataaataaaa tttgtatacc cattaaaata aatccatttg
     5041 ctatatcctt gaaattcttt cataaaacta taacctatta atgttgaagc atatatatca
     5101 atgaaaacca ttacaataac cccaaatact gaaccatata ctaaatcatc cataaccatt
     5161 gtaggcttat ttattagata gccacacatt ataccaagta atacactctg acttgcccaa
     5221 atagcagttc ctagatttcc aagtaccaga gaaattatta aaagcgaagt aattccagat
     5281 aaaatggtat actttaaatc acatttcaag cagattatac aaaaaaatac tggcactata
     5341 aaatctaagt aaatagcata tccaaatcca gtactcacag ctataattgt tactactatg
     5401 aataaagatg acaataaagc tgcttcagtt aattttttag tagtattcaa aacactgcct
     5461 ccataaatca taaattttct aactaaagtt ttgtttttaa tatagtatat cactttttaa
     5521 aaatattttt aataaatata aattttaata aaggtattta tgttaaaaat ggtataatgt
     5581 atcttaatat tcagtttttt aagatgctag gaggtaatta ctatgtttaa aattaaacac
     5641 tttaatgatt tgagtttaga tgaattttat gaaatagcaa aatcaagata tgaggtattt
     5701 gcttgtgaac agaagatatt ttcattaaac gattatgatg atatagataa aagctcttat
     5761 cacatatttt taaaagaaaa tggattgatt tgtgcttacg ctagaataat tcctaaagaa
     5821 tacagctctt ataatgatgt ttccattggt agagttttag ttctttctag tcatagaaga
     5881 aaaggattag caaaacaaat gatggattgt gctatagatt ttataaaagt taacttacac
     5941 gaaaataata taaccttatc agcacaaact tatataaaga acctttactt atcttgtggt
     6001 tttaaagaaa tctcagaagt atacgacgaa gcaggtattg aacatattaa aatgagatta
     6061 tagtgttgac aagctaatat tgttaatata taatttaagt ataaattaaa tattgaattg
     6121 atatttaggt atttgaaaat accttacata gagtcagaag gcttttgctt tctgactttt
     6181 ttattatcaa aattcaataa ttatatggat tcaaagtgtt aatttttatt ctatattcta
     6241 ctttccccaa aagtaagaat tatacccaac tttgtatgat aggcatcttg cctatctttt
     6301 ttttgtaaaa gtaacaaggt aataatataa tgcatagttt aaaataagaa gattagccca
     6361 ggctaaattt caagcatgga ggtgctatta tgaaaaacct taacgatatt caagtgaaaa
     6421 aaactgtcaa agttgaagat atattatcta gtggtaattt aagagaaaga atgttagcgc
     6481 ttggtcttac tagaggagct gttatagatg tagttagaaa aggtccaaaa aataatttaa
     6541 ctgtctataa aatcagaggt agcaagatag ctctacgaca agaagaaagt agcttaatat
     6601 tagtttctga catttaaaaa tttctagcag gaggatatta atatggggtt gacacataac
     6661 tcaactaaaa tgagttcttt aaaagacatg tttgatatag acaataagga agaccagttt
     6721 gtaattgcat tggctggcaa tccaaataca ggtaaaagta ctgtatttaa tcatcttaca
     6781 gggcttagac agcatactgg aaactggcca ggaaaaactg tagctacagc tcgtggtaat
     6841 tttaaatata aaaataccga atatgcatta atagatttac caggaacata ttctttattt
     6901 gccttatcac aagaagaaat tgtagctcgt gattttatat gttttggtaa tccagatgca
     6961 gtaatagtag tatgtgatgc tacatgtctt gaaagaaatt taaatttagt atttcaagtg
     7021 atggaactta cagataaagt tattttgtgt attaatttaa tagatgaagc tagaaaaaaa
     7081 ggcattacta ttgataaaaa acttttagag gattctcttg gtattccagt tattttaact
     7141 gctgcaagaa atggctctgg tatggatgaa ctattggata ctttaaatga tgtatctttt
     7201 gacaaataca aattaaataa taaacctgtt agatacaatg aaaatataga gaatgtggta
     7261 aaatcaattc aaccagaatt ggataatatt ataccaggta taaattctag atggttggga
     7321 cttagactta ttgatggcga tgaaagtata tttgaatcta tgagtaatta tatagacaaa
     7381 gattctatag atgctattaa tgaagtaaag aaaaagattc cagacaatat aaataaacag
     7441 aaaataagag atgagtttac taaaatcaac tatgattatg ctaaaaaact gagtgatgag
     7501 tgttgttcta atgttgctaa aaaatcaact gatagagaag aaaaagtaga taaaatatta
     7561 acatctaaaa tatttgggct tccaataatg ctcttattat taggtactat attgtggatt
     7621 actatagaag gagctaacta tccatccact ttattatcta atttattact cggatttgag
     7681 ccaagtattt cgggtatatt aaatagtatt aattgtccat catggttaaa tgatatgttg
     7741 gttttaggat tatacagaac attagcatgg gtaatctctg taatgctacc tcctatggca
     7801 atattttttc cattatttac cttacttgag gattttggat atttgccaag agttgcattt
     7861 aacttagacc acctatttaa aaaagcttgt gcacatggaa aacagtgttt gactatgtgt
     7921 atgggatttg gatgtaatgc agctggagtt attggttgta ggataattga ctctccaagg
     7981 gaaagactta tagctatact tacaaacaac tttgttcctt gtaatggtcg ttttcctact
     8041 ttgatagcta tatctacaat attttttagc tcagttataa ccaactcttt cgtatcaagt
     8101 gtagcaactg cactttgcat aactttactg ataattctag gtgttataat aacattgttg
     8161 gtttcttata ctctttctaa aacgctatta aaaggagttc cgtctacatt tacattagaa
     8221 cttcctcctt atagagttcc tcaaatagga agaactttat atacctctat aatagataga
     8281 acaatatttg ttttaggaag ggctgtaatg gtagccatac ctgctggtgt cataacttgg
     8341 atatttgcaa atatatatat aggagattta agtatattat cacatgttgc aaacttttta
     8401 gaccctttag ctaaattaat tggtcttgat ggttttatat tacttgcttt tattcttgga
     8461 ttcccagcaa atgagatagt tgtacctata cttttaatgg cttacctagc aactggttct
     8521 atgatagaat tagatagctt tagtgcttta ggtcaagttc ttagagaaca tggatggact
     8581 tatttgacag ctttaaatgt tatgctgttc agtttactgc attggccttg tgcaacaaca
     8641 cttttaacaa taaaaaaaga aactggtagt ttaaaatgga ctgccttagg attcttaatg
     8701 cctactatac ttgcttttgt tgtttgtttt ttaacaacta cagtttacaa tttatttata
     8761 taattagtat taactgacat tagacatata ttagagaaac tatttaaaaa tttatatact
     8821 ttattataaa gcatagagat atattcaatg ataagtaaaa ttacttaaca tttgaatata
     8881 tctctatttt aattcttatc tggatacttg gattgaaatt tcagtcatat aattatcttc
     8941 attactttca caaaaaaagt caagtatact ctcctcatac gaatttccaa taattttata
     9001 ttcattttca tatatatatt ttttcaattt ttcgtaagat ttatatgaat cctcatattt
     9061 tccaatatgt tttatgcaag catacattcc ttctggcttt acatatagct ttttatcctt
     9121 tattttttta tctatcttca aataaaaact tgatatactt aaatagtccc cattttttat
     9181 attttcatta cttactatta ctcccaactc atatccttga tacaaatttt tcgatttaca
     9241 ataattggtg aaatctatta attccatcat aaaatcacta ttactcatat tactcaaatc
     9301 gcttgataga actaaatatt cttcaaactt tttggatata taaatttctt cactattcat
     9361 tttagaaatt ccttcaactg ttatatttat tctattttgc atcttagaac ttatctttct
     9421 tagttttttt attttttctc ttatcttttt ttcttcttct ttaagcattt tcacacaaag
     9481 ctggggagtt ctattttgta tatactcttt tatttcctct aaactcattc ctatttctct
     9541 caatgattgt aatatatcaa gtgtatcata ttgataaact gagtaatatc tataattatt
     9601 tttatcttta tattcaggag aaaaaatacc tattttgtca tagaaaatta atgtctgctt
     9661 agatatacca cacaatttag caaattcacc tgttgtaaaa tactgttttt ccaagggtta
     9721 tacactcctt ttctttttgt tgtctatata gttactatat tctttattat ttactaaatc
     9781 aagtagttat tattttagaa aatcaatatg gttttgattt taaagcttaa atgtcagtta
     9841 gatgtcagtt ataaatattt tatccattcc catcataaaa aagatatccc ctcttaactt
     9901 ttggatggaa tattatattt atacttgtta gagtgaaaat tgtaataggt gctacaataa
     9961 gatatttaat taaacataaa gttaattatt gtaataaaaa atctttagat aaattagagt
    10021 taaagtatgg aaatatagat agagaaaaag ctgttaaact agaaatattt tatcagtatt
    10081 taatagaact tgaatatata gaaatgagtt tattaataag aagattagat actgcaataa
    10141 catcattagc attagtatct attataacaa tagcatcata ttatttaatc agaaaaaatg
    10201 tgtaattatg taagctattt ataataaaac actgataaaa ttaataataa aaaggtaata
    10261 ggatttctat actacctttt tgtttttata atatagtgta attttattat atttttgtat
    10321 gctaatgtaa ctagataatc ataagataac acattaaaag aaaattatga aaacttacat
    10381 tctgtatagt taatataaaa cataagtaat ataaatttat gtaaagcaac aaaagataga
    10441 tttacattcc atatagttaa tataaaacga agtttacatt tttattttag aatataagtc
    10501 gttgtaattt acattccata tagttaatat aaaaccttag agaaagcgaa aacgtaccat
    10561 taaaatcaaa tatttacatt ccatatagtt aatataaaac ttgtagttaa tattataatt
    10621 ccaactccta taagtataat ttacattcca tatagttaat ataaaactat tgcttaaact
    10681 tttaagacaa ggtggagctt aaaaatttac attccatata gttaatataa aactatccaa
    10741 ccaattctat atttatcatt ccaagaacga tttacattcc atatagttaa tataaaactt
    10801 tcctctaaag tccaaacacc ctctagcttt cccgtattta cattccatat agttaatata
    10861 aaacaccaaa ataaacttag tattttcaac acttacacgt atacaactct cctaaatttg
    10921 cagtgaacga gcaatagtgc aattgataac gcttatcatg cactctcaac gccttatatt
    10981 tcaactgtta cgccaaattt tttcacaaat atcgctcact gcaaaattcc tataatttta
    11041 ttatatcata aatatattac ttttgaatat tgataccaat ttataccata ataaaagcaa
    11101 aaaaacgtaa tctatctaca ttagatttaa attctataac ttaaagtttt ttacataata
    11161 tttatgcttg tttaatcaat ctaatatatc attcttgtat attgctttta atagtctttt
    11221 ttcttaaata aatatctatc tgaacaagca tagtttaatt aaaaactttt gtattccctt
    11281 aaaactgtat ctacctctta ttattttatt tatgatatat tatttttaat ctaaaaatag
    11341 atatctattt ttattatggt tatccctttt taaaatactt taatcatata ctattcccaa
    11401 tttaacatcc attgaaatgc tattttctta tattgcaatt tataattgat atatcttcaa
    11461 tataataatt taatgtattt ttgattttaa agcttttcag tgatattatt gataaattag
    11521 aattaattat ctagctttaa aaggtcaaag atttctgttg ctgtctgcgg ttttccaaat
    11581 aaatatcctt gtatgtaatc tggctttatt tgtttaacta aatcatactc ttcttgtgtt
    11641 tctacacctt ctaaacatac ttttattcct acatcatgac aaattgcaac aataaaatgt
    11701 ataaatgtag catcaaactt actctttaat atatctttaa caaatactct atcaattttc
    11761 actatatcta ttggagaaaa ttttaaaact tctagagacg agtatccagt accaaaatca
    11821 tccatagcaa tataaattcc taaatcttgt aaagccttaa atttaaactg taaaatactc
    11881 atattttgta ctgtatgact ttctgtcaat tctaaaacaa gattttgata aggaaaattt
    11941 tcatcatcta taatttttac tatatcttca ataaatgtgt cttctaatat ttgaacagct
    12001 gaaacgttta cactcacttt aaaaaaagga tagtaatcaa tccatttcct aaaagtacgt
    12061 aatgccattc gaagcaccca agcccctaca atatttatca tatcattttc ttctaatatt
    12121 ggaataaatt ccaaaggaga aattgctttg catttgtcat tagtccacct taaaagaact
    12181 tctacaccta ttattttatg actttctgta tctacttgtg gctggaatcg aagactaaac
    12241 cccctaaaat catcattaat tgatgccttt aaatcccgca tcatttctaa tgaatacatt
    12301 ttatttttta atatttcttg ggagaaaaaa acaagcctgt ttttaccatg ctctttcgca
    12361 tattgtaaag agtaactagc acacttataa agttctttta ctgtatctcc atgttttgga
    12421 tatattacac aaccagcaga aattgttata tttaatccat attttctcca tagttgaagg
    12481 tgtataatca tattctgtat ttgattatat agagttagaa tttcattttc ttcaacatta
    12541 tcaacaagaa tgcccatttc gtccccatca agtttataaa gttcagcatt atctggtaaa
    12601 atagattgta cagactgtgc tagtgttttt attatattat ctccaaaatc cctatcatac
    12661 atctcattaa catttttaaa atcgtcaata tctaataata ctatgcataa tttttcaatc
    12721 ataggatttg aaattttatc ttcaaataca ctcataaatt catgataatt cagtaactgt
    12781 gtcaatgaat caatcttatt ttgtttacct aattgcgtca ttattcctgc aaaaatactt
    12841 ggctctccaa attcatcacg cattagttga cctctacatc ttaaccatat atattctcca
    12901 ctacgatttt tagccctaaa ttccactgta tgataatcca tctggttttt acctatttca
    12961 gtgtttgact tataaaaacg attccagtct tctggatgta caattttttt ccagtaaaca
    13021 agcggatttt taacaatttc tcctggtaag tcaaataatt caacttgtga tggtgaatat
    13081 cgaaatacgc ctgttttcat attgcaaatg tatatatatt catctgtacc tctaatcaaa
    13141 gcatcgtaaa gcaatgtaga attataatca aaattgtctc ttgaaaaatt aaaaactcct
    13201 ttttcttctt tattatttga tatttcatga aattcattaa tcttaattgg tttatttaag
    13261 cttgttgagt ttgcaatatc tattgattga tgcatatgaa caactgtgcc atcttgccat
    13321 gtaattaaac tatcataatt ttcaaaaaca cgatttaact tattacattt ttcataccac
    13381 tttattaata ctcctttttt atcattcttt aataattcta aaactttaca aaaactacaa
    13441 gtagaatttt tttcaggata taatacttgc caacaaactt ttccttctgg atctaaaata
    13501 ttatattctt ctttcatttt tttgttcata aaaataattt cattagtatg aatatttgta
    13561 acatatatat taatctgtag ttgatttaat ataacttcaa aattatgttt gttcatatac
    13621 ttatctcctc catagttact gctctttata acaacattgt taaaattatt ctttttagaa
    13681 gatttactta taacttatac atatacacta aataccttaa tttaattaga aagtttatta
    13741 cttcat
//
LOCUS       DAENCV010000099        13444 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAENCV010000099 DAENCV010000000
VERSION     DAENCV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.100"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            202..2061
                     /locus_tag="KQD82_002857"
     CDS             202..2061
                     /locus_tag="KQD82_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4381316.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2353..2952)
                     /locus_tag="KQD82_002858"
     CDS             complement(2353..2952)
                     /locus_tag="KQD82_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4381317.1"
                     /translation="MAIELKKGQKINLTKKENSNLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3055..3852)
                     /locus_tag="KQD82_002859"
     CDS             complement(3055..3852)
                     /locus_tag="KQD82_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4381318.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3965..4070)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4162..5148)
                     /locus_tag="KQD82_002860"
     CDS             complement(4162..5148)
                     /locus_tag="KQD82_002860"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG4381319.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5632..7362
                     /locus_tag="KQD82_002861"
     CDS             5632..7362
                     /locus_tag="KQD82_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG4381320.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7472..7552
                     /locus_tag="KQD82_002862"
     tRNA            7472..7552
                     /locus_tag="KQD82_002862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7506..7508,aa:Leu,seq:tag)
     gene            7887..8402
                     /locus_tag="KQD82_002863"
     CDS             7887..8402
                     /locus_tag="KQD82_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG4381321.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YKNYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8894..8998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9208..11682
                     /gene="gcvPA"
                     /locus_tag="KQD82_002864"
     CDS             9208..11682
                     /gene="gcvPA"
                     /locus_tag="KQD82_002864"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG4381322.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11682..13139
                     /gene="gcvPB"
                     /locus_tag="KQD82_002865"
     CDS             11682..13139
                     /gene="gcvPB"
                     /locus_tag="KQD82_002865"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG4381323.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         5054 a         1591 c         2207 g         4592 t
ORIGIN      
        1 taagagtaat aatctataag aacttatata ttagaaaaaa ttttgtatat atagaggact
       61 ctaaagatga actatgtaaa acaaggttta tatattctaa ttgtatagaa aatttattgt
      121 aaacgcatgt atattattag aaaaaatagt ttgaaggtat tcaataaaca taaaaaacaa
      181 tataggggat aaaggtgttt tatgaagaga tttttgagga gaattatctt agttttattt
      241 attttgttga tttttataag ttttatttct attaaattaa ttcataatgt tggagattat
      301 ggaaaattga ttaattatgt tgggattgtt cgtggagcta gccaacgtct aactaaacta
      361 gagatgaatc atcaaccaaa tgatgaatta atagaatata ttgatgaaat cctacaggag
      421 ttgattacag ggcatggaga ttatggttta gtaatcactg attgtaatga atacaatgag
      481 gacttacttt tattagaaaa aaagtgggaa gatttaaata ttgaaattaa aaaagtaaga
      541 atgaaagaac agaataacca gttattaagt ataagtgaag agtttttttc tcttgcaaat
      601 gatacagtgt ttaaaataga aaacttttct aaagaaaaaa gtaattactt aatgacttta
      661 ataataatta tatctattat aggtatatta gcatgtatca tattgatttt acaatatagt
      721 aaaaaaatgg ttagactgga aaaattaaat gtagatttaa aaaatattgc atataaagat
      781 gaactaacag gagtaaatac tatagaaaaa tttaaattgg atgcaaatca aaatatttgt
      841 atacataagg ataagaaatt tgctgtattc tatattgatt ttgaaaactt taaatacatt
      901 aatgatatat ttggatatga ttatggtgac atgatactga aaagatatgc taatcttatg
      961 atgaatgaca tagggaaata tgaaatattt gcaagagaaa ttgcagatag atttgttgcg
     1021 ttaagatgct atgtagataa ggaagattta gtggtaagac aacgagatgt agacaatgaa
     1081 ctaattaata taacaaatga aattaaaaat aaacatagta ttactgttgt tagtggaatt
     1141 tgttgtattg aagatgtaaa tgaaaaatta agtatagatg gtttaataaa tagagcaaat
     1201 tttgcacaaa aaactgttaa gaataaacca ggtactaatt atgcttttta taatgacagt
     1261 attcgtaaaa aaatgattga agaaaatact atcaagagta gaatacatga agctattgaa
     1321 aaaagagaat ttatagttta tttacagcct aaagttaatt tacatagtca aaaaataaat
     1381 tgtgcagaag ctttagttcg ttggcttact ccggataaag gtattatatc tccagcaata
     1441 tttattccag tacttgagaa gaattttttt attgccttag tagataagta tgtttttgag
     1501 gaggtttgta aatggattag aaagaggtta gatgaaaata aaccttttgt tcagatatca
     1561 gttaatgttt caagaataca attttataac actaaatttg tagaaactta ttccaatata
     1621 caaaataaat acaggatacc taaaaatact atagaaattg aatttacaga gagtgttgct
     1681 tttgagaatc aaaaacatct tttggaaatt atacatgatt tgcatgaaaa tggatttact
     1741 tgttctttag atgattttgg aaaaggttat tcgtctttaa gtgttttaaa agatttacca
     1801 tttgatgttt taaagcttga ttctatgttt ttcaaggcta gtttagataa agataaagag
     1861 aaaatagtaa taaaaaatat tgtacatatg ttaaaagaac ttaatatcac aactgttgct
     1921 gagggcattg aatgcaagga acaagttgaa tttcttaggg atattgggtg tgatttagtt
     1981 cagggatttg tattttataa accaatgcca atattagaat ttgaaaaaat attggataaa
     2041 gagtttgttt ataatagcta aaatgtactt tataaaatat gaactaatta agtaaaatta
     2101 ttttgtactg aataaatttt tactttattt acagaataat gattatttac agaaaagcgc
     2161 gaaaagtgta ttcctttaag tgtgggatgg atagcacaaa tagaggagaa aaaatattgg
     2221 ccatataaga atgcataata atataaatat ttgaaaggtc agcattatat aaaaattaaa
     2281 aagaaaataa ataatttttc tataaaaaag agactatgag aaaatttact catagtctcc
     2341 taaataatgt atttattttc ttcctgcaac ccattttaat ccccaatgaa attcagtatc
     2401 catttctcta tgacctttga agtatttaac tactttttca acactgaatg tctcatcatt
     2461 tacattttct atcaatgcta ttgaacacat attatagcca tttttatgtt catcaagttt
     2521 gacaactaag tctgaatctt gtttttgttt gattgttaca attccatcag cttcagacca
     2581 gttagctaca ccttcgtata taaaagcata aataagtatt ctttttatat cttttatttt
     2641 attaccattt attcttaaat tttctccttg tgtattactt cctgttctgt catctccatc
     2701 taattgagca aaaggtggat tgtctaaact accaaaggca tttcctaatg cttgaacagc
     2761 accttttaca ccatttttca tctcaaatag gcaacccaaa tctaaatcta tattactgct
     2821 tcttaaagag ccaaaaaaac cctttttttg tactttttga ttccaattta aattaactaa
     2881 gatttcacct aaattgctat tttccttttt tgtaaggttt attttttgtc ccttttttaa
     2941 ttctatagcc atagtaatct tcctccacaa acatagattt atttaaatgg tagcacttaa
     3001 taagtcaata ttcaacaaga ttagaataat aatattaaaa ttatactaaa tatactaaaa
     3061 agtaggaatc acaatacctt tgtatttttc ttcaattaag cttcttgctt tatctgatgt
     3121 aagaacatct tgtagaactt taatcttttc actgtcttta tcttcagttc taaatgctaa
     3181 aagatttgca tactcgtcat ttataggagc tgtataaatt ccgtttttct tggcatcaag
     3241 accagcaggt acagcataag tagtatctat aaatgcagca gtcacatctg gaagagaaga
     3301 tggaatttga gcaatatcta actctttgaa ttgtattccc tttggatttt ctattatgtc
     3361 cttaggacta actaatccat cccctttagg taacgttaaa agtccaatac tttcaaggta
     3421 tctaaggttc ttagaacaag aagatgggtt attgcttatt gcaattgtat ctcctttttt
     3481 aaattcatca acagatttta tcttataaga gtaaagtatt gctgggcata catatagttt
     3541 tttaccagcc attattttgt aacctttggc cttaacagct tcttttagat aaggttcatg
     3601 ttgaaataga tttgcatcaa tttcaccatt atttaaagct tcgtttggaa gtatgtaatc
     3661 atcaaagttt ttaacttcta aagtatatcc tttttcttta ataagtggtt ttaattcttc
     3721 taataactct cctcctggaa ctaaagtagc accaacaaca atttttttat catctttagc
     3781 tttagaacat ccaaccacag ctaaagttag tactagacat aataacaaac ataataattt
     3841 tttgaatttc atataaattc cctcctgaat ataattgatt agttaaaaat aaaataccat
     3901 tctttatcac actgcaaaat aaaaataagc ctgagtttaa actcaggctt taaaagctaa
     3961 tttagactca tctttcagta taatactgca ggatttagca ccaatgtata taaaaatata
     4021 ctggttgctg ggtttcatag ggccagtccc ttcaccactc tggataagaa gtatcaattt
     4081 ttaattatgt tataaatatt atcatgattt tttacgtctg taaacataat tttataaata
     4141 ttttaaaatc tttaatgtat tttatttaac acctaaaatt ccagaaacta gaatatcagt
     4201 atcagcacct aagaaatagt catttatttc aatagaatcc agagtatttc tcaaagcatt
     4261 ttcttcatgc cttataccaa taagtttatt ttctacaaat gcaacatcac attttccaaa
     4321 aaaatctcca aaaaatttta tactagttat tatgccttta tcaacattta aattaaactc
     4381 aacatttcca ccagaatatt ttaattcatt tgaaagagta tattttggtg aattaccaaa
     4441 attccaatcc caagtttcgt attttgattt agtaagcttt tctatttgtt ttatatcatg
     4501 ctcactcaaa caataattag tattatcagt atttgtttta gaaagataat ccattatagc
     4561 atctttaaat tctaatatgt ccatttttga atgtaaatgt tcacttatat tggttacacg
     4621 agattttaca gattttattc cttttccttc aaattttata ggtttaacct ttaatgcttt
     4681 tgagaggtca tttatttcag atgaaaataa aagagtccca tgatgcatta ctttgttttt
     4741 gtagttgtat tgagcatttc cagaaaattt cttgccatct ataagaaggt catttcttcc
     4801 agaaaattcg gcattgacat ctagtgtttt aagtaaatct attatgggct gagtgaatct
     4861 tctaaaatca ctaaaattat tattgttaca agctataaat gtaaaattta tatttcccaa
     4921 atcatgaaat acagctccac caccagactg tctcctaacg acaggtatag aatattcttt
     4981 tatatattct aaatttattt cagaaagtgt gttttggttt tttccaacaa ttatagaagg
     5041 ttcattcctc cataaaataa ataagtcttc atcaaaattt tttagaagat attcttccat
     5101 tgctaaatta aaataagggt ttgttttttc attataaatt aataacatta aagtagttcc
     5161 tccatagaat tgataaaatt tttattatga atacaattaa ttttataatt cgacaataat
     5221 ataaaaactc ctttaaatta gaaattttaa aaactacctt gcaaagttta aataaataat
     5281 atatactcaa aatatgttta ataaaagtga atagaagggt agaggcgcaa taatctatca
     5341 gtactaataa ggagttaagc acagagagtt attaggaaag gagatattgc cgaagtttta
     5401 taatcgatgc tttaagatta tattgctggg ggtacataga aaatatgtac aactgtcaca
     5461 ggtttatttg tggtgagcta tcatttaagg aattatatta tatttatttt tactattgtt
     5521 gatatattgt aatgtttaag ccttgagtgt ttaccactca aggctttttt agttttattg
     5581 taacataata ccccgtacag ttcacaaaat taaaaggaga gggagattta tatgaggaac
     5641 aaaaaaatta atattatttt tctgacgaca atcatgttca taatgtcaac agtaatggtt
     5701 tttgcagaag aagatataga cactattgca ctagccaatg cagaaaaatt tgggatttta
     5761 actttaatac caccattagt tgctataata ctcgcattta taactaaaaa cgtaataatt
     5821 tctttactta taggtattct ttctggtagt tttataatta aagctagtgg tattaatgtt
     5881 tttgcgacat ttatacaggc atttttagat ttagtagaca gagctttagt atctttagca
     5941 gacccttgga atgcggggat aatattacag gttttggcaa ttggaggagt aataaatctt
     6001 gttgctaaaa tgggaggagc taaagctata gctgaagcac ttgctaaaag agcaaagtca
     6061 gcaaaaggga cacagcttat aacatggttt ttggggcttt tagtattctt tgatgattat
     6121 gccaattcac taatagtagg tcctatgatg agacctgttg cagataaaat gaaaatatcg
     6181 agagaaaagc tagcatttat aatagatgcc acagcagcac cagttgctgg tttagctata
     6241 atatctacat ggataggtct tgaggttggt ttaatacatg atgcttttga aagtataagt
     6301 atagatgtag atgcatttgg tatattttta aatacaatac catttagatt ttacaatata
     6361 ttaatattag ctttcattgt aatatcagca ttattattaa aagaatttgg tccaatgaga
     6421 aaagctgaga taaaatctag aagtagaaaa attagtatag accttgatga aggcgtagaa
     6481 gagttggatg atttagctcc aaaaaatgga gtaaaattga gtgtatggaa tgctataatc
     6541 ccaataggaa cattgataat agttgcatta gcatcatttt attatagtgg atatacatct
     6601 ataatgggag gagacgataa ggctctgata caactattta caaattctcc atattctttt
     6661 gaagcaataa aagaagcatt tagtgcatct gatgcatcta gagcattatt ccagtctgca
     6721 cttgttgcaa gtttagttgc tattataatg gctgtagtaa aaaagatttt tacaatatca
     6781 gaagcaattg atgtttggat agatggaatg aaaagtttag taatcactgg tgtaatatta
     6841 atacttgcat ggtcattaag ttcagtaata aaagagttag gtacagctaa atttttaata
     6901 cacctattat caggttcact accaccattc ttattaccaa gtttaatatt tggtttagga
     6961 gcaataatat cttttgctac tggaacagct tatggaacaa tgggtatact tatgccactt
     7021 gcaattccat tagcatactc attaaatcct gatatgtctt atgtaatagt aagtactagt
     7081 gcagttttaa caggtgcaat atttggagac cattgttctc caatttcaga tacaacaata
     7141 ctttcttcaa tgggagctgg ctgtaatcat atagaccatg ttaacactca aatgccatat
     7201 gctatattta cagcagtaat cactattgta tttggatata taccagctgg acttggattg
     7261 ccaatatata tagtattacc agtggcaata gctgctatat ttgtaggtat tcaaataatt
     7321 ggtaagaaag ttgatgaagc ggaaatagaa cttgtagaat aaagatataa ataagtttca
     7381 ccttttatta tcattatttt gttagttttt agataatttt aaaaagttat tgaaaataat
     7441 ggcgaaatat agtaaaataa aagagtgata agcggatgtg gcggaattgg cagacgcact
     7501 agacttagga tctagcgcca ttggcgtggg ggttcgactc ccttcatccg cactttacag
     7561 tacttaattg tactgaaaaa aactggggtt gctggctttc ggagtgggct atcccgtttt
     7621 tatttttttg tatatataat agattggcac tttagtacaa agtgcttttt ttataggcaa
     7681 aaacagtaca tttatttctg ttttattatc attcatctat tagaatatac tttagttaag
     7741 aatgtgttaa taagcagtgt tcacattctt gataaaatgt ggaataatat gtatttttac
     7801 taagacggaa atgaaataat taaaccatta aataatatta taagatgatg aataagtaga
     7861 taaatcaaat tttgtaggag gatattatgg aaaaatatat atggttgttt ccattacttt
     7921 ttatttttca tgatatggaa gagataatag gttttggaat atggcttaaa aaaaataaat
     7981 caatgcttga taagaagtat ccatttatca gtaaagttta taaaaattat agtactgaag
     8041 gcatggcatt tgctgtgttt gaagaattta ttttatgcat tattttctgt atacttacag
     8101 ttataacaga gaatcagtat gtatatctac tatggttagg tagttttata gcatatacct
     8161 tacatttggt aattcatata gggcagtcaa tcataataag aaaatatata ccttcactta
     8221 ttaccagtat catatgctta ccaataagca tttggtgtat aagtaagagt atttatatag
     8281 ttgattgtga aatgagtaca acaattctgt acagtataat tggcattata attgtagcat
     8341 tgaatttaaa gtttgcacag tcgctgattg gaaaatttac aaagtggatg tcaaacatat
     8401 aaattttagt ttattggaca gttcaaggaa tcagaaattg gaattttata gtattaatat
     8461 tttatagtat taatataatg tataaaattt tatagcaatg tatttcataa gtataaagtg
     8521 actagaattt taagaaatat acaaattcta taaaaactaa ataatttcaa gtatatttta
     8581 aaaatcttat ttttcacaag tttaaaattg taaacgattt catcaagtct atgaatatac
     8641 ttttaacatg ttatagtaaa tctcaatata aatttacatt tggttctttg tgtgttattt
     8701 taataataaa ttaagtgaaa atgttatttt acattaatat ataatatctt tgcataagag
     8761 tgaataatat aaaagagaaa taattgataa taaaaactta acaaaaaaat taatacataa
     8821 aaaaaaataa atataatgtg ttgacattta attgaaatat tcatacaata aaattgaaaa
     8881 taaatatttc caaatgagat tagcgagaga gtcctttata cgaaaaggcg ccgaagaaga
     8941 aatttaagaa atgccaattt ttagagaagc tttcaggcaa aaatatcgct aattgatggg
     9001 actctggaga gacttaagtt aagatactat aagtcaccga agaagcaaaa taatattaaa
     9061 ttattaaatc tttcaggtat acaaacaggg atatataggc taggattatt ttccatgctg
     9121 atatatcttt tttttatgaa aaagtacagg aaataatatc aaagttactt aaatacagta
     9181 ggtctaattt tctagggagg gttatatatg aatgaattga aaagagtatc tttgtacaat
     9241 atacacaaag agcttggagc aaaacttgtt gaatttgctg gttgggaaat gcctttagaa
     9301 tatgagggta taaataaaga gcatgagaag gtcagaaaaa gtgcaggtat atttgatgta
     9361 tctcatatgg gagaggtaca gataaaggga gcagagtcag aaaaatttat acaaaaccta
     9421 gttacaaatg atataagcac attaaagata aatgacatta tatatacacc tatgtgttat
     9481 gaaaatggtg gagtagttga tgatttatta atatataaat ttggtgaaga agattattta
     9541 ttagtaataa atgcaggtaa tatagataaa gatgtagcat ggataataaa acaaagtgaa
     9601 ggatacaatg tagatataaa aaatatatct agtgaagtga gtcaattagc aattcaagga
     9661 cctaaagcag aagaaattct tcaaaaaatc acagatatag acttaaactc aataaaattt
     9721 tataagtcaa ttccatcaac aaaagtatgt ggttgtcctt gtttagtttc aagaacagga
     9781 tatactgggg aagatgggtt tgagatatac tgtaaaaata aatatgtaga aataatttgg
     9841 aatgaagttc taaaggttgg aggagaagat atatgtccag ctggtctagg ttgtagggat
     9901 actttgagat ttgaagcagc tctacctcta tatggacatg aaataaatga acacatatct
     9961 ccaattgaag gtggtttatc tatctttgtg aaaacaaata aagaatcatt tattggcaaa
    10021 tcaattttat caaaagaaaa agaaagtgga gctaaaagaa agttagtagg atttgaaatg
    10081 caaggtaaag gaatgcctag aaatggatat gatataagga ttggtgataa aacagttgga
    10141 tttgtaacaa caggatgtgc atctccaacg acaggaaaaa ttttaggtat gggaattata
    10201 gattctgaat atgcaaaagt aggaaatgaa ataggtatag caatcagaaa aaaagttgtt
    10261 cctgctgtga tagttaagaa acctttttat aaaaaacaat ataaaaaaga taatattata
    10321 ttaaacaaag aaaataaatt ttcatatata cctgctacaa gtgaagataa atcaaaaatg
    10381 ttaaaagtag ttggattaaa ttcagttgat gaattatttt cagatatacc agaagaagta
    10441 aaattaaaaa gagatttaaa tttggaaatt ggaaaatctg aattagaggt aagcaaaata
    10501 gtaaaaagat tgtcagaaga aaatttaagt ttagaagact tgacttgttt tcttggtgct
    10561 ggtgcatatg accattatat accatcaatt ataaaacata taacttcaag atctgaattt
    10621 tatacagcat atactccata tcaagctgaa ataagtcagg gaactttaca agtagttttt
    10681 gaatttcaat ctatgattgc agaaattaca ggtatggaaa ttgcaaatgc atcaatgtat
    10741 gatggagcaa ctgctgcaat agaagcatgt ataatggcaa tgaatcagac tagaaaaagt
    10801 aaaatagtag tgtcaaaaac tattcatcct gaaactctgt cagtattaag aacttactta
    10861 caatataaag attgtgagat agtagaaatt gacttttgta atgaatatgg gactacagat
    10921 atagaaaaat taaaagcttc tgtagacaaa gatacagcat gtgttcttat acaaactcct
    10981 aatttctttg gaattattga agaaatggaa gaaatagaaa agataactca tgaaaataag
    11041 gctatgttaa ttatgagtgt agacccaata tcattaggag ttttaaaaac accaggtgag
    11101 ataggtgcag atattgttgt tggagaagct caatcacttg gaaaccctct taattttgga
    11161 ggtccttatg taggattttt agctagcaaa tctaaatata ctagaaaaat gccaggaaga
    11221 atagttgggc aaagtttaga tgtagaaggt aaaattgcat atgtattaac tttacaaact
    11281 agagaacagc atgtaagaag agaaaaagca acttctaata tatgttcaaa tcaagcttta
    11341 aatgctcttg tagcttcaat atatatggct actatgggta aagaaggatt taaagaggtt
    11401 ggtatgcagt ctatgaaaaa agcacattat acctacaata aacttgtaca aactggaaaa
    11461 tataagccaa tatttaaagg taagtttttc aaagaatttg ctgtacaagg aaatcttaat
    11521 atagaaacta taaatgataa actcttagaa gaaaatatat taggaggtta taatttagaa
    11581 tacaattatc cagaattaaa aaattcaact ttgctttgtg tgactgagaa aagaagcaaa
    11641 gaggaaatag ataagttagt tggaataatg gagggattat aatgaaagaa tacaatagtt
    11701 tgttgataga tatatctaaa aaaggtagga aagcatactc acttccaaag ttagatatag
    11761 atgatataaa aatagaagat atgattgacc cagatatggc aagacaaagt gagttgaatt
    11821 tgccagaagt tggtgaatta gaattagtta gacattatac attattatca aataaaaact
    11881 ttggagttga tacaggtttt tatcctttgg gttcttgtac tatgaaatat aatcctaaaa
    11941 taaatgaaga tatggcagca cttccaaact ttactggaat gcatccatac caatcttcgg
    12001 atacagctca aggttctctt tctttgatgt atgatttatc aagaagactt gcagaaatta
    12061 caggtatgga tgaagttaca ctacaacctt cagcaggttc tcatggtgag tttacagggc
    12121 ttatgattat aaaagcatat catgaaaata gaggtgacca taagagaacc aaagtgataa
    12181 ttccagattc tgcacatgga acaaatccag caagtgcagc tatggcaaac tttgatgtga
    12241 ttcaaatagc atctgataaa aatggagctg tagacataaa tgcattaaaa gaggttttga
    12301 atgatgaagt agcagctctt atgcttacta atccaagtac tcttggttta tttgaaaaaa
    12361 atataaaaga aatagctact cttgtacatg aagcaggagg acttttgtac tatgatggtg
    12421 caaacatgaa cgctattatg gggataacaa gacctggaga tatggggttt gatgtggttc
    12481 atcttaatct tcataagact ttttcaactc ctcatggagg tggaggtcca ggagctggtc
    12541 ctgttggagt taaaaaagaa ttagtacctt ttttaccaat accagtaatt gaaagaaaag
    12601 aagataagta tgtactaaat tatgatagag aaaaatctat tgggaaaatt aaaaatttct
    12661 atggtaattt tggagttctt gtaagagctt acacatatat actaactatg ggaagggatg
    12721 gacttaaaga ggctagtgaa atggcagttt taaatgctaa ttatataaag gaaagtataa
    12781 aagatgatta tattttgcca atagatacct tgtgtaagca tgagtttgta ttaggtggat
    12841 tgccaagagg agaggccaat ataaaaacta tcgatattgc aaaaagacta ctagattatg
    12901 gataccatcc tccaacaatg tactttcctc ttattataaa tgaggcactt atgatagaac
    12961 ctacagaaag tgagagtata gagacattag atagttttat agaagctatg aaaagtgttg
    13021 ctaaagaggc aaaggaagaa ccagaactat tgaaaactgc tccacataat actttggtca
    13081 aaagagtaga tgatgcaagg gcagtaaaga aacctatact aacatggtca caaagataaa
    13141 tagttaaaaa gtagagattt aaagtataaa gagaattaaa tttgtaagaa aaagctatct
    13201 aacttaatct agtttagata gcttttttgt atattagtat gtatggtatt ttctatataa
    13261 agttttaaat tgtattagtg cttatataga atattgatat gatgattatg tatagatatt
    13321 aataagaatt gttgaattta tcttgcctta actttattta ctcttttata ttaagaaaaa
    13381 cttaactaaa atatagcgaa attcataaaa atttaggaaa tcttaagaat tataatattt
    13441 aaat
//
LOCUS       DAENCV010000100        13410 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAENCV010000100 DAENCV010000000
VERSION     DAENCV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.101"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            306..1106
                     /locus_tag="KQD82_002866"
     CDS             306..1106
                     /locus_tag="KQD82_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG4381324.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGIHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            1633..3873
                     /locus_tag="KQD82_002867"
     CDS             1633..3873
                     /locus_tag="KQD82_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG4381325.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEAEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            4285..5820
                     /locus_tag="KQD82_002868"
     CDS             4285..5820
                     /locus_tag="KQD82_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG4381326.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMKNGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            5836..6597
                     /locus_tag="KQD82_002869"
     CDS             5836..6597
                     /locus_tag="KQD82_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381327.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            6935..7804
                     /locus_tag="KQD82_002870"
     CDS             6935..7804
                     /locus_tag="KQD82_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4381328.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            8133..8384
                     /locus_tag="KQD82_002871"
     CDS             8133..8384
                     /locus_tag="KQD82_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG4381329.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            8496..9275
                     /locus_tag="KQD82_002872"
     CDS             8496..9275
                     /locus_tag="KQD82_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4381330.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(9410..10060)
                     /locus_tag="KQD82_002873"
     CDS             complement(9410..10060)
                     /locus_tag="KQD82_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381331.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(10057..10560)
                     /locus_tag="KQD82_002874"
     CDS             complement(10057..10560)
                     /locus_tag="KQD82_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4381332.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILSNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(10609..11124)
                     /locus_tag="KQD82_002875"
     CDS             complement(10609..11124)
                     /locus_tag="KQD82_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG4381333.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            11404..11931
                     /locus_tag="KQD82_002876"
     CDS             11404..11931
                     /locus_tag="KQD82_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4381334.1"
                     /translation="MHEQIDYCERKKIRTINIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            11915..13318
                     /locus_tag="KQD82_002877"
     CDS             11915..13318
                     /locus_tag="KQD82_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381335.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFIFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPGQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         5040 a         1473 c         2325 g         4572 t
ORIGIN      
        1 taataataga catatttata ttaaaaaata agtttaataa atttttaagc ttaataaatt
       61 ttaagaatta agaagctttc tggctgtaat gtttgcaatg ttgcagttag aaggcttttt
      121 tattttatta tagttcgtgt caaagattgt atgaaacaat aaaatattgt aaaataattt
      181 gtatatttag acttgattta cacaaaggtt tataagatat ttgagttgat tatacgaaat
      241 tctatgaagt agtaaccctg tgactttaat catgggaggt tcagaagaag gagaaaattt
      301 taaatatgga aagtaaaacg aagtacatag gaataagtat attaatatta ttgatgatat
      361 ttttaattgc aaagaatttt aacggaaaac ttgaaatacc tattcctgag agtaatagta
      421 gaaaatttaa atcagcagca gtgttttatc ctcaacatca agatgatgag gttttatggg
      481 gaggaagtgc cattgtagat gctataaaac agtgtggtgt tgataatgta tatgtggtat
      541 tagtatcaga tggttctgga gttaacgttt tcaaagctaa cacaaaattt agaaatttaa
      601 ctaggaagca aaaggaagaa cttagaaata acgagtttaa atcagctttg agagagcttg
      661 gagtgaaacc tcaaaatgtg attatacttg cagatataga taaaaaggaa ggcatacatt
      721 atgaattaat ggaaaagact atattggatt ttgaacataa gtttaaaggc aatgtgaccc
      781 atatagccca tcactataag tatgatgacc atataatgca tagaaagaat ggggaagttt
      841 taaagaaact taaacgtgaa ggtaagataa aagatgactt gtattttgtt aagccaaaat
      901 atgtaaagta tataccaaga aatgaaagag ctatctataa agcaatggat tttgatgact
      961 acaataaggt taagagtgct tgttatgaat ataaggttgt aaattcaaag cgtaaaatgt
     1021 atggtatagg atatatttcg gcacatagtt attttgaaca tcttctaaat gaccctgaac
     1081 ttacgtctgt attaagtgaa gattaatttc ttataagtag taagttatgt ttgataagtt
     1141 gattggaatt ttctatagtt ttgaaggtat tggaaattta gttttattta aagatttaaa
     1201 gatagaggtt tttagtgttt aaaattttaa agttaagttg agtaattgta tagaattatt
     1261 tgtgaaagaa gaaacagtgt aaaacatagt atcttattta tggttttaaa atcataggca
     1321 agatactttt tttatgtaac taataccatt ttgagattat taacttaaga attttatata
     1381 atttaacact catatataat gatgatattt aggtagaata gatgcaatta cgcttaaata
     1441 aaataactgt attttttata aaaatcaaac cctacactat actaccagat aaaatctaca
     1501 tgctataatt tgacctgtgt ttataaaaaa aggagtaatc taataaaata gtatttcact
     1561 tatgtataac taacaactat tattacgatt ttctctaaat aatttaaaga aaaataatgc
     1621 aggaggtaaa atatgaacaa tgaatttata attttaaaag gttgcaaaga gaacaatttg
     1681 aacaatattt ccttaaaaat accaaaaaga aaaataacca tattcacagg ggtgtcgggt
     1741 tctggaaagt catctattgt atttgagaca attgcaaaag aatcacaacg ccaactaaat
     1801 gaaaggttta gtacatttgt aagaagtttt ttacctaagt atggagaggt taaagctgat
     1861 tgtatagaaa atttgtcaac accaattatc atagaccaaa gtagacttgg tggaaattca
     1921 aggtcaaccc taggtactat tacagatgta aattcctttt taagagcttt atattcaagg
     1981 tttggtagtg agtatatagg taatgccaat atgttttctt ttaataatat tgatggtatg
     2041 tgcccacact gtcatgggct tggaaaaaag ctagttccta atatgaacca aattcttgat
     2101 atgaataaat cattaaatga aggtgctata ttgctttcag ggtttggagt gggttcttgg
     2161 cattggaata tattcgctga atcaggattt tttgatatta ataagaggat atgtgattat
     2221 tcagaagtag aattggaaaa gtttttgcat ggtaaagcag aaaagattaa gatagaaaat
     2281 gctggtcaaa ctaacatgac atatgaggga ctaatggtta aatttaatcg cctatactta
     2341 agtagagagg gagaaatatc tgaagctact aagaaaaaat tgagtaaact tcttatagaa
     2401 gatagatgtc ctatatgtga tggaagaaga cttaatgaaa gagtatatag gtctttaatt
     2461 aatggttata atatagcaga tttaactagt atgcaaattg atgaattggc tgaagttatt
     2521 aaaagtatag atgaggcaga agcacaacct ttaattaaag gaataataga aaagttaaac
     2581 agtatcatag atataggtct tggatattta acactggata gagagacatc ttctctatca
     2641 ggaggggaat ctcagcgtat aaagatggtt aagtatttaa atagtaatct tgtagattta
     2701 atgtatattt ttgatgaacc aagtgtagga cttcatccaa aagatgtgta taaattaaat
     2761 aatcttttaa aaaaattgcg tgataaaggg aatacaatta tagttgttga acatgaccca
     2821 gatgttatta agattgcaga ccatattgta gatgtaggac caaaggcagg taagtatggt
     2881 ggagaagttg tgtatgaagg cagttatgaa aatctactta caagtggtac tttgacaggg
     2941 aatgctttaa gtaaatttct tcctattaaa gaaagtgtaa gagaacatag tggatattta
     3001 gaagttaaaa attgtaataa aaacaattta aaaaatgtat cgattaagat acctaaaggt
     3061 gtattgactt tagttactgg agtagcagga gcaggtaaaa gtacgttgat taaggatgaa
     3121 tttttaaagc aaaatcctag tgctgtactt atagaccaaa gccctgtttc agcaaattca
     3181 aggtcgagtt tggcaacata tagtggaatt atgaacaaca taagaagcat attttcaaaa
     3241 acaaatggcg taaatgcttc tttattcagt tcaaattcag aaggagcttg tgataactgt
     3301 aagggttctg gaatagtaga aatgaatctt gcttttatgg agagtataaa gagtacctgc
     3361 aatgtttgtg aaggtaaaaa atttaaaaaa gaagttttag aatataggtt tcaagggaag
     3421 aatataattg aagttcttga aatgtctgtt ttagacgcaa ttgagttttt taatttaaaa
     3481 cagattaaaa cgaaattaca atgtattgaa aaaatgggta ttggatacct tactttagga
     3541 caaactcttg atactttgtc tggaggagag tgtcaaagat taaagcttgc tagtgaatta
     3601 aataaggaaa gttcagtgta tatattagat gaaccaacaa caggtcttca tatggctgat
     3661 attgagaatt ttataagtat aattgaagag attgtagata atggaaatac ggtcataata
     3721 attgaacaca atatagatgt aattaaaaga gctgattgga ttattgagct gggacctgaa
     3781 ggaggaacaa aaggcgggag agtaatattt gaaggtattc caaaacaatt atgtaacaat
     3841 aaaatatcat taacagcaaa atatatagta taataaataa aatataataa aattataatg
     3901 atattttaat aaatataatg ggtatataaa atgataatta aacttaacag taataaataa
     3961 tgtgagaaat attcaaattt aattaaataa agtgtcagaa atttgtttaa attttagtat
     4021 tttaacggtt ctgacctttt ttcttatttt tatggagaat atcgaggatg gagtctctaa
     4081 tagttacttt tttgtgtcta cttgcaagtt tgattaaaaa atggctatac acacttgtga
     4141 cttcagtcat gggaggttcc gattataatc ttaatataat ttgtgaaata atttgatgac
     4201 gatttgctga tgtataaaca ataaagatta actacattaa aatttaaatc ttgttaagta
     4261 aaacttataa aaggagagag atggatgata aatgatattc taaccttatt atctgaaaga
     4321 agtgattttt ttataaagct atccatagaa catttgatga tttctattat ttctataata
     4381 attgcgattg ttttgggtgg tataattggt attattgtaa gtgaatttca taattcttct
     4441 aaacctatat tgggaactat caattttcta tacaccattc catctatttc tatgctagga
     4501 tttctgatac cattttccgg aattggaaat actactgctg ttattgcact taccatttat
     4561 gcattattgc caatggttag aaatacatat actggaatta ctaatgtgga taaaaatatt
     4621 cttgaagcag ctaggggaat gggaagtaca gattttcaga tattgtataa aataaaactt
     4681 ccgttagccc tacctgttat tatgtctggt attagaaata tggtgattat gacaattgca
     4741 ttagctggta tcgcttcatt tataggtgct ggaggcctag gtgtagccat atatagagga
     4801 atcacgacaa ataacacaat aatgacaatt actggaagta tacttattgc tctattagct
     4861 ttaatatttg attttatttt agggctggta gaaaaatttt ttcaacgaaa aagaaaaaag
     4921 aaaaatagaa aaaaagtttt tgccattcta ggaagcctta ttcttatagc aactatcttt
     4981 acgtcttatt tatttaatcc ttcaaagact caaactattc atattgctac taaacctatg
     5041 actgaacaat atattcttgg tgagatgttg gatattttaa ttgaaaaaaa cacaggtcta
     5101 aacgtggaaa ttacacaagg tgtaggaggg ggaacatcta atatccagcc agctatgaag
     5161 aatggagaat ttgacattta tccagagtat acaggtacag gctggaatat ggtacttaag
     5221 aaaaatgaat tatatactga aagtatgttt agtaaactac agtcggaata caaaactaaa
     5281 cttaaaatgc aatggactgg tatgtatggt ttttctaata gctatgcatt agcagttaga
     5341 aaagaagttg cagataaata caatttaaaa aatataacgg acttaaaaga tgtatcttct
     5401 aaattgacat ttggagctga atatgacttt tatgagagag aggatggata tactccattg
     5461 tgtaatacat atggactgaa ctttaaagat acagttgatt tagatattgg attaaaatac
     5521 caagctatca atgaaggtaa gattgacgta atgcctatat ttacaactga tggtcagtat
     5581 agtgtttcag atgttgtagt tctaaaagat gataaaaatt tctacccatc ttatcaatgt
     5641 ggaaatgttg ttcgtttgga agtattagag gaaaatccag aactatcaaa ggtatttaag
     5701 atggttggag atattttgac tgataaagaa atggctaaaa tgaattatga cgtagaaaac
     5761 aacaatttag aacctagaga agttgctcgt gagtttctga ataagaaagg tcttttataa
     5821 attggaggaa atataatgac acctattata caatttaaaa atattaaaaa acaatataat
     5881 gataaaacta taatagataa tcttaattta gatatagaaa aaggagagtt tttgactgta
     5941 attggttcat ctggttctgg taagacaaca ctcttaaaaa tgattaatgg tctgattttg
     6001 ccagatggag gaaatatttt gataaaccaa acagatatca aaaacgagga cttaattaaa
     6061 ttaagaagaa gaataggata ttgtgtacag ggaagtgttc tttttcctca tatgactgtt
     6121 gaagaaaata tttcatatgt accaaatctc ctaagtaaaa agaataaatt agaagtaaaa
     6181 agtgctgtaa acaaatggat ggaaatagtg ggattgccaa atgatatgaa agtgcgttac
     6241 ccatcagaat tgtcaggtgg tcagcaacaa cgtgtaggga ttgcaagagc tttagcttct
     6301 tctccagaaa ttttattaat ggatgaacct tttggtgcag ttgatgagat tacacgaaag
     6361 caattacaaa aagagataaa agaaattcat aaaaaaacag gagttacgat tatctttatt
     6421 acgcatgata tttatgaagc tttgattttg ggaacaaaga ctttggtatt aaatcatgga
     6481 gttgttcaac agtatgatac tcctgagaat attttaaata ctcctgctaa ccaatttgta
     6541 gaccagttat tgaatataag aaaatctata atagatgatg ataatttaaa agaataaatt
     6601 taaatttaaa atcaaattgt tgtgataaaa actattgatt tagagctaac ttcaagttgt
     6661 atacttgaag tggaaaagtt agattaaggt atagaaggga atttagcagt tggctgtaga
     6721 caaggtaact gctgaaggtg atttcaataa atggctagaa cctgtatctg ttgaagatta
     6781 taataaattg aaataagaag ctcaatatac agagtagata ggcacttcaa atagaagtgt
     6841 cttttgtgtg ccataaaata aatcaaaata ttagcaaaga atgtgaaaaa caaaataaaa
     6901 taatatgcta tctttaaaat aggaggggtg ctatatggat aacaatttga ttaccattga
     6961 aaaaacttta gattatatag aaaaacatct tttagatgag atgttagatt taaatgtaat
     7021 ttctaaagag ataggctatt caaaatacca tttacataga atgtttacat cagttgtcgg
     7081 atttagtgta cataattata tacaaagacg tagacttact gaagcagcga gaatgctaat
     7141 ttttacagaa aaaccaatta ttgagattgc tttaatctcg gggtatgaca cacaacgctc
     7201 tttttcaaga gggtttaaaa aaatgtttaa acgtagtcct aattttttta gaagacatca
     7261 agatttttta ccactacaat taaaatttga tattcataat agaaaaaaat tacgtggaga
     7321 tatggtttta gatgttaaaa aagttaattg tgcagatata aatattgttg gctattctgg
     7381 taaagtaaga aaaggatttc atattattgg aaaatgttgg agggatttgc ataaaaataa
     7441 agagatgata gaaaataagg tagatttagg ttttttaatt ggagttagtg attatagcaa
     7501 ttttaaagag attgagaata ctcctacatt tacatacatt gcaggtgctc aagtagctac
     7561 ttttggcaaa ttacctaatg gaatggaaaa attcgtatta ccgaaaagta attatattgt
     7621 ttttactttt attgggaaaa atgaggatag tatgcaaaat gttgtagaat atatctatca
     7681 agaatggttc cctaattcaa gttgccagtt taatgaaaat aatttatatg actttgtaaa
     7741 atatggagaa aaaactgatg agaatggttt gagtgaaatt cagttttggg ttccaatcat
     7801 ttaaaagaag gtattatgta taaagtagat ggatttatat aggcaaagtt ttacagcagt
     7861 atgtatttat aaataattta tagtttgtag gaaagaaata attttactat aagattatga
     7921 taaaatacac aatactatga caaaatagtg ttaaataata gatttaattt tgcataggaa
     7981 aagttctata atggtgtgta ttcataaata atttataatc tgtaggaaag aaatagtttt
     8041 actataagat tatgataaaa tacacaatac tatgataaaa tagtactaaa taatagattt
     8101 aattttgcaa aggataaggt gttttaataa aaatgaatgt agaaaacgaa ttacttaaaa
     8161 acctagatag attgcataca acagagttag gcgttgtaag aattaagaaa aatctttctt
     8221 tggagacaaa tgatgtagta aactggtgta aaactaaaat agaatctcca aatgccatta
     8281 ttaatagaaa gggaaaaaac tggtatataa gtgtatatga ttgtattata acagtaaacg
     8341 ctcacagtta tacaattatt acagctcata aagaaaagaa atgaattgca aaatacaaag
     8401 gtcaaaagta ttttccactg tttgtagtac ctatgaagaa gtatataaaa taatattact
     8461 tttgaataat aatttaaatt ttggaggttg aaaaaatgaa tgatactgta aaagaaaaat
     8521 tggcagagtc actaacttct gatactactg atattttacc ttatttgcct tatttattac
     8581 aagatttttg ggaattagga agttcaccag aacaaatgtt acaattattt agaaagcata
     8641 taccttttaa tgaggataca aaagttttgg atttagcttg tggaaaaggt gctgtatctg
     8701 tgaagcttgc aaaagagttg aatatacata tcaaaggtat tgatttgata cctgaattta
     8761 ttgattatgc gagaagaaag gctattgaat ataaggttga tgctttgtgt gattttgaaa
     8821 tgaatgatgc taatagttct gtaaaaattg agcacgatta tgattgtgtg atttttggag
     8881 ctgtagggga tattttaggt aattataaag aaactataag taagctatta cctacaataa
     8941 aaagtaaggg gttcatttta attgatgatg catatgtagt agataaatct acaaatgaac
     9001 agcttaattt tgagagggtg tatcccactt acaatgagtg gtgtatattg ttcaaagaat
     9061 taggtctgaa actaattgaa tgtataccat gtttagaact tgaaaatgat gttgaagagg
     9121 aaatgaactg gattataact cgtgcaaatg agctaattaa aaaatatcct tctcaaaaag
     9181 atatgtttga acaatatgtt attagccaaa aaaatgaata tactgattta cagcaggacc
     9241 ttgttggagc aacttggatt ttacaaaaag aataagataa atattagact agataatcaa
     9301 tgttgtagtg aattatccca tgtctagtga atataaataa cataaaaaac cgtcaagatg
     9361 aatagtttat tcgtgcttga cggtttttag tatttgttga agtaaaattt tatttcttta
     9421 gtatatcact tttagtcaat ccatttgatt tagcctgagt attccattga tgattcccca
     9481 taagtttata taggaatcta gggaagttag gtgaaataaa aatagtactt ttgttggaat
     9541 catctatatg tatattatca ctaagtatat tttttgaaag ttcttttaat gcaattccta
     9601 aatctttttt aggtccttta cccaaaggta cggattctag tgaaccaaac atctccccag
     9661 ctccaattcc aataccttga acccatgtaa agttgatttt tttacaccaa tgctctatca
     9721 tttgaattgc caaatgatta tgttcacctt cattgaatcc acagttaact attgcataca
     9781 ctggaatagg ttttttattt tgtgttttta aaaatttttc acatttctct aaaaaatgaa
     9841 gtaaatgaga cggaatacca tctacataaa gaggaaatgc tattattaat ctatcacaat
     9901 taagtatggc attaagagcg tcaggatact tagatgtgct atctacttta aatgtaaaaa
     9961 ctttattatc attttgaata agttgtttta attcagataa aaaatactgt gaattatttt
    10021 tatttacttt tggactacca tttatcatta ctatattcat atggattcac ctcttatatc
    10081 ctgtatattt tcatagaaac aaacagagtg ttcttttata ttgaaattta tagcatttcc
    10141 atttactaat ttcgtagcag tttctttttc agcttctgta atacaatctc catagaatat
    10201 agcttttaaa tttatctgtt tatcatatct gcttttatga cgcatcatat tatcttttat
    10261 tacaaagtat ggaagaatat aattaatgct tttatcaagt acgtttttga caaaagggct
    10321 aaaccctccg taacagcatc tgcttattat aataattcca tcagattcat ttataagttt
    10381 acccatgttg ctgtaataat cttttacaac acattcagaa ggagttttaa tccagcaacc
    10441 aaaacaacca atacatttat ggatttcttt tgtgtttgaa agtattttta tattgtcatc
    10501 aagtttgggt aataactttt ctaattcttt gtcagttaaa tcatgtatta aaagcctcat
    10561 aaagtccatc tccttttatt atgtatgttt acttctaaaa attatatctt atagattttt
    10621 cagtaaatct ttacaccaat taataacaaa attataatag tcaactccat aaatcattgt
    10681 tgcatacata tatgtatatt ggtcaacaca atgattttct tctgcatcat ttttttgttt
    10741 acttaattta tttagaagta attctacttc agttattaac atttttaagt ttgtttcaat
    10801 tatttctttg ggtagatact tgtaaaagaa tatattaaca aggtggtctt gctttgtttt
    10861 agaaaaattt ataggtttct ttaaccattc aagaaatact tctttaccag tattagttat
    10921 ggaatatagt ttttttaatt tgctaccatc tataacttcg tgatagtgta tatatccttt
    10981 tgtttctaat cttttaagtg caggatagat acttccaaag ctggcgtcaa aaaagtacga
    11041 ggtactttct gacattattt gttttaagtc gtatccactt aattcttttt ccattaaaaa
    11101 tcctaaaata atgtattcta acatattacc tcctgatatc aattagatat atatcgattc
    11161 gatatattta ttattacaga taataaatct tacgtcaaga tgctttttaa aataagctaa
    11221 aaagaattag gagttattga taaatatgca aaaaaattaa tataaaaggt aattaaatat
    11281 caatgaagat ttaaaaatag ttaaatcaaa gtaagagtct aatatataaa atttatagta
    11341 actttatggt ttaaatataa acttgtgtgc tattgacaaa gtataaaatt agcagtaaaa
    11401 taattgcatg agcaaataga ttattgcgaa cgcaaaaaaa taaggacaat aaatattaaa
    11461 ggagagaata tgagagagct attaaaatgg gtttccatta taaatagata caatacaaat
    11521 tatattgaca ggaaattggc ggatattgga atcaataata gccaatattt ttatgtatta
    11581 cacatatgta ataatccagg tattacacag gacactatat ttgagaaaat tcttgtgaat
    11641 cctagtaata taacaagagc tttggcaaat ttagaaaaag aaggctttat aacaagagaa
    11701 ccaggtgtaa aggataaaag aacatggcat ttatatccta cagaaaaatc tttagaatgt
    11761 tatgatgaaa tactaaaaat aacaaatggg tatgttgagg agttgttgag ttcatttgag
    11821 aaagaagagc aagagttgtt tatgtcaatg ttaaagaaag ttgcattttt ggctattgat
    11881 atgaatgaag aagcaaaatt ggaggcgaag gaaaatggaa gaaaaatata aggagccatt
    11941 ggaatatgaa aaggtttcaa agttattaac taagtacgca attcctagta ttatagcaat
    12001 gttagtaagt gctttatata acattgttga ccaaatattt attgggcaag gtgttggtgt
    12061 attgggtaat gctgctacaa atgtcacttt tccactaaca acaatatgta ccgcaattgc
    12121 attattgtta ggaattggag gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc
    12181 agaaaaagct acaaaagcag caggaaattc tatatgcttg atggctgttt ttggaataag
    12241 tttatttata gtagtatcaa tatttttaac accactatta aaattctttg gctctacatc
    12301 agaaatacta ccttatgcac acacatatac aaaaattaca tcaattggat taccatttct
    12361 tataatgtcc actggaatga gtaagttgat acttgcagat gggagaccaa aagcttctat
    12421 gttttgtatg ttaataggag cagttattaa tacaattttg aatccattat tcatttttat
    12481 ttttaatatg ggaattgctg ggtctgcttt agcaactgtt attgggcaac ttatatcttt
    12541 tggaataagt gttcattata taaagaattt tcaacatatt aaattacata agaaaagttt
    12601 taaattagat agtataattt ataaacatat atttgcgcta ggttcaagct catgttttaa
    12661 tcaattagca atgactgtaa cacagatagt tatgaataat acattggcat attatggggc
    12721 taggtcagtt tatggaagtg agattccact tgcttgtgtc ggtatcatta ttaaagtaaa
    12781 tatgattttt atatctataa ttattggtat atcacaaggt atgcaaccaa ttatagggta
    12841 taattatggg gctgaaaagt ataatcgtgt aaaagaagca tataagtttg cagtgggaag
    12901 tgcaactatt atatctgtta ttgcattctt atgtttccaa ttcttcccag gacaaattac
    12961 gtctatattt ggtaacggaa atgagactta ttttatattt gcagaacatt attttagaac
    13021 tttcttgttc ttaacattta taaatggtgt tcaaatagta tcatcaactt tctttacatc
    13081 aattggtaaa tcagtgttgg gactttttac ttctttgact cgtcaaattt tatttttaat
    13141 tccattgata gtaattttac caatgtttat gggaattgat ggagtaatgt ttgcaggacc
    13201 aatagcagat gcagcagcgg caatggtatg tatatacttt gcagttcatg aaatgaaatc
    13261 attgactata aagcaaaata atttgatgag taaccataat aagattgcag aggtataaga
    13321 caatatttaa gaagtataga aaattaattt aaagaagtat aataaactaa tttaggaagt
    13381 atagaaaatt aatctaaaga agtataataa
//
LOCUS       DAENCV010000101        13339 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAENCV010000101 DAENCV010000000
VERSION     DAENCV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.102"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(146..799)
                     /locus_tag="KQD82_002878"
     CDS             complement(146..799)
                     /locus_tag="KQD82_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4381336.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPMEEMKKEKLL"
     gene            complement(1044..2909)
                     /locus_tag="KQD82_002879"
     CDS             complement(1044..2909)
                     /locus_tag="KQD82_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG4381337.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDS
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2914..5334)
                     /locus_tag="KQD82_002880"
     CDS             complement(2914..5334)
                     /locus_tag="KQD82_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG4381338.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMIKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5348..6790)
                     /gene="glgA"
                     /locus_tag="KQD82_002881"
     CDS             complement(5348..6790)
                     /gene="glgA"
                     /locus_tag="KQD82_002881"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG4381339.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6819..7949)
                     /gene="glgD"
                     /locus_tag="KQD82_002882"
     CDS             complement(6819..7949)
                     /gene="glgD"
                     /locus_tag="KQD82_002882"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG4381340.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7951..9111)
                     /locus_tag="KQD82_002883"
     CDS             complement(7951..9111)
                     /locus_tag="KQD82_002883"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG4381341.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            complement(9882..10925)
                     /locus_tag="KQD82_002884"
     CDS             complement(9882..10925)
                     /locus_tag="KQD82_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG4381342.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(10946..11392)
                     /locus_tag="KQD82_002885"
     CDS             complement(10946..11392)
                     /locus_tag="KQD82_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG4381343.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(11566..13146)
                     /locus_tag="KQD82_002886"
     CDS             complement(11566..13146)
                     /locus_tag="KQD82_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG4381344.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         4238 a         2433 c         1270 g         5398 t
ORIGIN      
        1 ttgatttata tatgttaatt taaatacatt attctatcgt agtgccttag agtttttatt
       61 tgtatttaaa gttaaattta ttcttgtttt tcctttaata tttaatagta aaacaacaaa
      121 attataaaaa aaataaagag aaactttata aaagcttctc ttttttcatc tcttccatag
      181 gtatcgtttt ccaatgttct ctcaaaactc tttcaacatt ttctaaatca ttctccctca
      241 tagattttac tacttcaata tgttgattat tgctgtatgt caacatttta tataactcat
      301 cttcattatc actagtatat gactttgaca taaaattata tcttaaagag ttcaatagat
      361 ttactaacac ctgattatct gatttagtta tgtatatatc gtgaaattca ttacttaatt
      421 ttaaatagct attgtaattt ttatacttta tagagaccgc catcatttcc gttaattctt
      481 ccatttttac taggtctgat tcagttaaat tttgtaatgc tgaagaaccg gctaacgcat
      541 ccaatactcc aattacggaa taggtgtcta atttctcctt ttcctgaaat tcttttacta
      601 caaatccctt attaggtatt ttttctagta aattttcacc tgctaaccta gttaatgctt
      661 ctctcgctgg tgttctacta actccgatag atttacaaat ctcagattca gtaattctct
      721 gattagcttc atattctcca ttttggattt tctttgatat atagtcatat acttgagttg
      781 taagtggatc taccttcata agacgttcct ccatattaag attataaaag ttaaatctcc
      841 ctacaacttt ggtagagagt tcagttttat cttactaatt tatataatat cacaattatg
      901 atatttttta tacttctttg taatattttg aaatactaaa attcttatga taatttatac
      961 aaaaaaagta gaaaaagttt tgataaaaca aaactctttc cacattcacc ttattttcta
     1021 tttatttttc taaaaaaaga tatttaattt actctaaaaa ttctaaatga tttagagtct
     1081 aaatctactt tcaactcatt attttcaatt tgtatagtac tatttgcatt tttaagacta
     1141 tactttatag gtatttcctc aataaaacta ctctctatac ataatgatat actttccaca
     1201 ttttcacttc tatttactaa aattaacatt ctatcattat tttcattaac tctaataaaa
     1261 gaaaatacag aatcattatt agtatatata aattctgtat ttccagaact taaaagttta
     1321 ttcttatttc taatttttat aacatctcta taaaaattaa gcatatcttc gtcttcattt
     1381 ttccatggat aagttcttct attatctggg tcaacacctc cacaaagacc tgcttcatct
     1441 ccataatata tatatggtac tccttcaaat gtcatctgta tagctacagc aagcttcact
     1501 aaatctacat catcatttaa ttccgtttta gctcttgtaa catcatgtgt tccaattaaa
     1561 tttaaattag acctaaatga ttcttttgga taattttctt taatctgcat atatctatta
     1621 caaagttcat atgaattaat ttctcctttt aagaaagaaa acatgttatt tctaaaagga
     1681 taacccataa cagaatctaa ttcttcacct aataaataac ttcttctctg accatagcta
     1741 atcttatttg atgcatcttc ccatacttca cctataagaa ttgagtcatc atctgccttt
     1801 tttaattcct tttttaactc tcttataaac ttagttggta attcgtctgc aacatctagt
     1861 ctccagccct taatacccat atttacccat ttatttataa cgctatcatt gtcatatatt
     1921 atataatcca tatagctatg ttctaattca tttatatttg gcaatgtctt aacatcccac
     1981 caacttttat atttttgagg aaattcttca aacatatacc atgaataata tggagattct
     2041 ttagattgat atgcccctaa actattatag tttccataca tattaaaata tttactatct
     2101 gctccagtat gactaaaaac tccatctaat actattgata tacctttttc ttttgcttta
     2161 tcaataagtt ctttaaatgt atcttcatcc ccaaacattg ggtcaatttt tttatagtca
     2221 cctgtatcat acttatgatt actagaggct tcaaatatag ggcttagata tagtatactt
     2281 acacctaatt tttttaaata tccaagttta ttgattatcc ctcttaaatt tccaccatgg
     2341 aaatcccatc ttataacatc cccttgacta tcctttatgt acataggtgt atcttcccaa
     2401 tttccatata tgaaactatt ttttttagga ctatcaacct taccactacg atttccatta
     2461 ttgaatctat ctacaaatat atgataaagt attccttctt tgtaccatgt tggtacttta
     2521 aaatctttat aaacagtgat ttgatattta ttaatatcac tataattata ttcacaagaa
     2581 tatccattat ctctgttttt tccataaaat aaagtcttta tctgaccatc catttcaaca
     2641 tcaactttaa agtaataaaa ataaacacct gtaccatcaa acttttcaat cttaccacaa
     2701 aagcactttc caagtttatc attatctaat tcttcattta aagttattgt tctcatttct
     2761 tcttctctat ttatttctag ggaaatagac ttgatattgt atccctcatt tactttaaca
     2821 caaatggtca attcctcatc aaattttaaa gctccaaacg gagccttaaa atttttatcc
     2881 caagagttat attctattac actttgcatt taatcacacc cttttaaacc aaatgtcttt
     2941 tgcatactca gaaattgttc tatcagaaga aaaaaatcca gagttagcta tattaactaa
     3001 agacatttta ttccattttt ctttatctct atataacctg tttatatctg cttgagcttg
     3061 accataattt tcaaaatctc tcaatacaaa atactcatca ttgtaagtag tgagtgaatt
     3121 ataaatactt cttccttctt cgccaagatt aggaataaat ccatttatta aatcatctac
     3181 tacccttttt atatccatat tagaatgata taggtcaagt gaagaatatc caccatattt
     3241 attataattt agtacttgtt ctgcactaag tccaaataaa aacatatttt ctttaccaac
     3301 ctgttcacat atctctacat ttgcaccgtc aagtgtagcc aaagttatag caccattcat
     3361 cataaacttc atattacttg ttccagatgc ttccttagtt gtagttgaaa tttgctcact
     3421 aacatttgct gctggtatta ttatttctgc taatgatact ccataattct ctaaaaatac
     3481 tacttttaac ttgtccttta ctctaacatc attatttata gtacttgcaa cagaatttat
     3541 aaatttaatt atacatttag ccaaataata tcctggtgct gcttttgcgc caaatataaa
     3601 agttcttggg tcaagattca aattaggatt atcaagtaat tcatgataca tatgaagtac
     3661 attaaatata ttcagtaact gtcttttata cgcatgcaat ctcttaactt gaacatcaaa
     3721 tatcgaactt gggtctacat ttaaatcgta tttatctttt ataaactttg ctagatttgc
     3781 tttattattg tattttatat catcaagtct ttgtaaaaca gaggaatcat ttttaaattt
     3841 ttcaatattt tttaattgta atgtatcttt cttccatgaa tcaccaatta aatctgtaat
     3901 taaattgcta agttgaggat ttgaacttat aagccatcgc ctatgtgcaa ttccattagt
     3961 cttattatta aatttatttg gttcatcttg atagaaatct tttaatacct ctgtttcaag
     4021 aatatgagta tgaagcttag caactccatt tacactatga cttgtgacaa tacataagtt
     4081 tgccatattg atattgtcac agtctattat actcattctt tttattctat cctgctcata
     4141 tcctttatta tttaattcct ctacatatct tctatttatt tcttctatta tcatataaat
     4201 tcttggtaat agttctttaa tcatacttac aggccatttt tccattgctt ctgacattat
     4261 agtatgatta gtataggaca ctgttttttt agttatttcc catgcttcat cccaagataa
     4321 attttcttca tcaagcaaaa ttctcataag ttctggtatg cataaggtag gatgtgtatc
     4381 atttatatga attgctacct tatcgaataa attattaata tttaatttgt tctttttata
     4441 ttttcttatt atatcttgta tccctgcact tacaaaaaag tattcttgtt tcaacctcaa
     4501 caacttacct gcataatttg aatcatcagg atataatacc tgtgatattt cctcagtata
     4561 atacttatgt tttaaagcct cttcatagct accagaataa tttagagcat ttgaagtaag
     4621 ttcaccaaaa tctctctttg gtatttcact cttaaacaat cttaaagtat ttatacattg
     4681 gttttggtat ccgattattg gtatatcata aggcattgcc attacaggaa ggtagttttt
     4741 atgaattact tttaaatgac taccttcctt gattaattct acctcaccac caaacttcac
     4801 cattgttgct tcatttggtc ttacagtttc ccatgcatat cgtccttctg taagccagtt
     4861 atcaggcact tctacctgat aaccatttac aaatttttgt tcaaacagac catatttata
     4921 ccttattcca tatccttgac cacttatgtt caaagatgcc atagaatcta aaaagcaagc
     4981 tgctaatcta ccaagtccac catttcctaa ggctgggtct acttcagctt caattaaatc
     5041 atccaagttg attcctaatt cagatagacc ttctctaact tcttcttcaa tatttaaatt
     5101 taataaatta gattttaact gtctccctat taaaaattct aaactaaaat aataaacttc
     5161 ttttttctta tctaactttg tagcaaccca tttttttgct atctcatctt tcaatagact
     5221 acataataca tttaacaatt gttcatcagt agcttccttg atagattgtg catataagct
     5281 atacattttt acttcaaatt tttgcttgaa tttctttttg cttattgtta ccaaaaaaac
     5341 cactccttta atcacatatg tctctatatg tttctatata ctccttagct gattttttcc
     5401 aactattatc tgttttcatt gcattttcaa ctaatttaat ccatttttct ttgtccttaa
     5461 atactttgat tgcatttttc aaacagaaaa acatctcatg agcattgtaa tttttaaaac
     5521 taaatccatt gccttctcca gtaaatttat tataaggtgt tactgtatct ctaagccctc
     5581 cagtctctct aactattggt aaagaaccat atctcatagc taacatttga cctataccac
     5641 aaggctcaaa taatgatggc attaagaaca tatcacttgc agcatatatc tgttgagcca
     5701 atgatgcatt aaaagtaatt ctagctgata ctttatctga aaaattagaa tcgtaatagt
     5761 tgaacattga ttgatattga tgttctccag ttcctaaaac gacaatctgt aaatcttctc
     5821 tcataagttc tggcatcatg tatgctatta aatcaaatcc tttttgagaa actaatctcg
     5881 aaactatacc tatcaacgga atagatgaat ctttttttag tcccaatata tcttgtaatc
     5941 tcaatttatt ttctactttt ttgtcaattg agtctacatc ataatgaaca aatatatctt
     6001 tatcttttgc agggtcattt aaatcataat ctataccatt taatatacct tttaattttc
     6061 ctgactcttt tctaagtaat ccatctaatt gttcgccata aaaagatgtt tgtatttcat
     6121 ttgcataagt agggctaaca gttataattt tatctgcaaa atttatacca gctttcataa
     6181 aatttattga gccataatac tctatatctc cattatcaaa gtatttttgt ggtaaatcca
     6241 atatatcata taaaacatgc ttatcaaata caccttgata ttggagatta tgtatagtat
     6301 acatagtttt tatattttta tacccctcaa gggttgcata tctttctttt agaattaatg
     6361 gtaacatccc tgtatgccaa tcatttatat gtattacatc tggataaaaa tcaattctac
     6421 ttatagcttc taaaactgca tctgtaaaga aagtatatct ttctgcatca tctccatatc
     6481 catagatact ctttctttca tcttctcttc taaaataaaa ttcattatct ataaagtaaa
     6541 attttacacc atctaattcc atttctaaaa gaccacagta ttgatttctc catccaacac
     6601 gtacactaaa cacagctatt tcttttagtt gatcttttaa ataacttggt atagttgaat
     6661 atttgggcat tattaccctt acatctacac cttctttttt taattcttta ggcaatgcat
     6721 aagaaacatc tcctaatcct ccagtttttg caaagggcca acactctgct gttacataaa
     6781 aaaccttcat tagtcattcc cccttctaaa aaatttattt atatatttgt attcttttcc
     6841 actacaagag gatatttttc atctcctctt aattctttcc catttgatac actcgtattt
     6901 ttatctaata ttgcattata tagttttgca tttgacttta ttacacaatt ttgcattact
     6961 attgagttct ctataactgt tcctttttca acatttacct ttctaaatat gatactatct
     7021 tttacagtac cttctattaa acaaccagat gctataaagc tattttctac tttagcacta
     7081 tctgtataaa ttgtaggtga ttcattcttt tcttttgtaa atatctttct ttcagaatat
     7141 aataattcat ctgcaacttc taaatttaat aaatctttgc tcatttcaaa atatgattca
     7201 acagaattta cacattttag atatcctgta tattcatagg ctcctatttt catgttttct
     7261 gcttcttcaa taataaattc ttctaaataa caataattgc ctttatttac aatgttatat
     7321 atacattcaa caaaatcttc acgcttcata atatatgtct ccatacatat gtttgctcta
     7381 gggaacttac ctaaattatt tcctacattt attaccctat tgttactatc taaatttaga
     7441 gttaagttac cagcaaaatc tttatttgca ttatttacat ttttatatat tatagttata
     7501 tcattatgag attttttatg gtattccaat gcttgagaat aatttaaatt gcatagcata
     7561 tagcttggag ctattaaaat atattcttct tcactctttt ctatataatc tagattagct
     7621 aagatagtat atatatcacc ttctctatgt gagtaagtat ttctatactt agtgttttct
     7681 ggactaaaaa ttgataggcc accttttttt ctacttaaat cccaatcttt accactacct
     7741 atatggtctg ttaatgatct gtatttttct tttgcgaata ttccaacttt agtgattcct
     7801 gaatttatca tattagataa agcaaagtct attattctat atcttccagc tattggtgta
     7861 gatgctattg gtcttccata gttaagtttg tttattgctg ggtctccttt tttgtttaaa
     7921 tttattatcc ctaaacattc atttctcatc ttaaagacct ccttctatta atacgcatct
     7981 aggttcgata ttttcatact cagaaactac atttatttca ttatttacat tctttatctg
     8041 aacattatca tttatagttg cacactcacc tatcatagct ttttctatag ttacattttc
     8101 acctattaca acatttggca ttattactga atcaattact ttacttcctt ttccaacaac
     8161 aactccatgt gataatacag agttacctac ttcacctaaa attctacaac catcagctat
     8221 cattgatcta tgaacatttg catttttacc tatatattgt ggaggcattg ccatagtatt
     8281 tgtatatatc ttccatttag ggtctgctaa atcaagtgtt tctggagatt ttattatatc
     8341 catattagca tcccatagac tttgtatagt tcctacatct ctccagtatc ctttaaatgg
     8401 ataagcatac attccaacat tatcttctaa cattttaggt attaaatttt ttccaaaatc
     8461 atcataattt atttctggat ttttttctgc ttccttaaaa taatttctca acattttcca
     8521 atcaaatatg tatactccca tagatgccaa attgctctta ggttcacttg gtttttcttc
     8581 aaattcatag atacttgaat cttcatttgt attcattatc ccaaatcttg aagcttcatc
     8641 ccaaggaact tcaatcactg ctattgtagc tttagagcct ttttccttat gaaattttaa
     8701 cattttgcta taatccatct tgtatatatg gtcaccagat aatatcaata catattctgg
     8761 attataggta tctacaaagt ccatattttg atatatcgca tgtgctgtac cattatacca
     8821 atttccttct ttttcattca taaatggttg taaaacatag acaccaccat ttattctgtc
     8881 caaatcccaa tgacttccca ttcctatatg tgaatttaat atcaacggtc tatattgagt
     8941 taaaacacct acagtatcaa taccagagtt tgaacaattg cttaacacaa aatctataat
     9001 tctatactta cctccaaatg atacagctgg tttggcaatt ctttttgtaa aaacacctaa
     9061 tcttgaacct tgccctcctg ccaaaatcat agctaacatc tcttttttca tttaatgaca
     9121 ctcccctcat ctagtaatta atatttataa ttgcattttt acttttataa actttatcag
     9181 tcaataaatg tcgtgtataa atgttgctta aataatacct taataaaaat aaaataatat
     9241 caactataca attgatataa tgtatacccc ataatgaact taccgacgta tacattattt
     9301 tccataagca ctatagtatt tattaatatt caattatcgg ttaaaaaata atacaaattt
     9361 tattttataa atataaaaac attttttata aatctcttgt gtcatatgaa catgcttatt
     9421 ttttaatttg ttaaattcgt aatagcaaaa tataatctaa tatttgttaa ataattatcc
     9481 aaggaaaatc aagtaattat taatcaactt tattttttaa ttgatacttc tttgtctata
     9541 atttaaacaa aaggatataa acttttaaat tatttattag ttttcttagt attaatgttt
     9601 atatttttat aaatatatat taataatata aatagactaa ctaaaaccat gcttatttgc
     9661 tatatgtttt atataatatt atataacact ttttacatta gtaaaggata aattgtataa
     9721 agattctgtt aatttatttt tataatttca tctttctaat aaaaacaaag aagaagactt
     9781 aaaaatttat aagtcctctt cttttcaacg tgcatttttg tgctatattt aattaattcg
     9841 acctttaatt tccatatcct tcctatgata acaaaactct tttatttttt taatgattca
     9901 ttatcattta aaacttcttt tatatcaagt acttctttgt tatcatcact cataacttcc
     9961 tttattcctt tgaatgttcc taggaaatta actgcttctg aaggtaaaac cagttttgtt
    10021 gacttacctt cagccatttt ttcaagagct tccatagatt ttaatgctag aatattattg
    10081 tctatatcag cctctttcat tgctgaaaaa actaatttta aaccttctgc tgttgccatt
    10141 tgagttcttt ttatcatttc agcttcacct tgtgccttag caatagctgt ctgtcttata
    10201 gcttcagctt caccttctgc cactaatata gcagattctt tttcaccttc tgctacacgt
    10261 accatagctt cttttttggc ttctgctgtt aatatagcag attgtttttc accttcagct
    10321 tgaagtattg cagctgactt attaccttct gcttgaagta ttgcctctct tctttctctt
    10381 tctgctctca tttgtttttc cattgcaact tgaatatcct gaggaggcat tatatttttt
    10441 aactctactc tatttacttt tataccccat ttatctgttg cttcatcaag gatagttctc
    10501 atttttacat ttattatatc tcttgatgtt aatgtttcat ctaaatcaag ttcaccaatt
    10561 atatttctta gtgttgtagc tgttaaattt tcaatagcag ctattggatt agctatctca
    10621 aacacatatc taactgggtc tgtaacttta taatatacaa ctgtatcaat ctgcatagtt
    10681 acattatctt tagttataac tggttgaggt ggaaaatcta ttacaatttc tctaaggtca
    10741 attacatatg ccattttatc taaaaatggt attaagaaat gtacacctgt ttcagctact
    10801 ttttgaaact taccaagtct cattattata cctactttag attgttttat aactcttata
    10861 caagttaaac ttattgctac gactactact attaatacaa tacttaaaac aattttagta
    10921 cccatactat aaatcctcct ttattctatt ggtttgtcac attaactgtg gcgtctactt
    10981 ttttaacaac taattttacc ccttcaattt ttaatacttc tactgtagaa cctttttcta
    11041 tcttttcacc atctatagaa atagccgtcc atgtttcatg ttcaactaca actagacctg
    11101 tgttatttgg agaaatctct tctgtaacaa tacctttttt agacatcatt gcttgtaaat
    11161 tagtgttaga tttatagcct ttatcttttt ttactatttt tttagtagct acgactaaca
    11221 ttgctataga aattactgca aatattaaaa tttgtaagaa aatactctct ataaagctac
    11281 ttaaaaatat aagtattaca gctcctacac taaaccatat caatgttaag cttggtgtta
    11341 acatttctgc tattccaaat aagactgcta ctatcaacca tattaacttc atactatcta
    11401 ccctcttttc atatgaatta taaaacccta tataaattat atgtttatac aatgtttttg
    11461 aatataaatt taaattacaa aacaataaaa taaataaatt aattaatagt attatatcaa
    11521 catactttgt taaaattgtt acaaagtagt atcaaatatc accaattata tatccaacaa
    11581 ctctttcatt gttaatgaaa tgttacttat taaatccgta gcatttaccg tatacattgc
    11641 tttgctcaat ttatctccta tatagccatg tatatatgct ccactaactg tagcttccaa
    11701 tatatccata ccttgaccta taaatgaagt aatcattcca agcaaacaat cacccattcc
    11761 accagttgcc atacagctat ttcctgtagg atttatatat gtttttttac catctgttat
    11821 gacagtttga taacctttta aaagcaccac cagattatat ttttgagcaa attcttttgc
    11881 aacatctact ctatgttctc ttatataccc aattgaatct cctgtcaacc tggccatttc
    11941 tcccaaatga ggtgtaatta caaatctatt tttccattct aaaaacttat aacatctgtc
    12001 cttcataacg ttcaatccat ctgcatctaa tactattggg caattagaat tttctactat
    12061 cttaagtaat ttatcaaatg tttgactatt atctcccata cctggaccaa aaccaattgc
    12121 atcactagaa ttaagtaatt tatttaatct ttcttcttca aaatttaaag tcattgcttc
    12181 tgtaagtcta atagatacct tttcttgcac atcttcacta ctaattaatg ttacaagtcc
    12241 actcccactc ttaacagcag attcagttgc tatgaaactt gcaccataaa atccttttga
    12301 acctgcaact atagatactt ttccaaaatc acttttaaaa ccatgcttat ctcttcttct
    12361 aatattattt tttacaaaag tcttatcagt tatgaactct tcatcatggt attttcttgt
    12421 aataaaatct ggaacaccta tcttttctac aactacatca cctataagtg tgctagtgtc
    12481 atagtttaga aaacctcttt tataaaactc aaagctaatc gttttgtttg ctctaataca
    12541 agttcctaat acatttccat tatcgctatc tatacctgaa ggaatatcta tagaatatgt
    12601 atttttacta ttatcgttta ctatgtcaat aacttcttta aaaataccag ttatatcacg
    12661 ttttaatcca gtcccaaata tagcatctac tacaacatca cttttagata ctctatcttt
    12721 aaaaattgtt aaatttttat tttttataat attttgtttc tcaactttaa taattttatc
    12781 tacttcattt tcatctccat aatttttttc acttaaatca tttttattaa tatttataat
    12841 attaattccc atatttaaaa gtacattgta atttatttta gagcattcac ttagtttttc
    12901 aagattacct actaaaaata cgtttacttc ttttccatat gtatttagat gtcttgctat
    12961 tccaagacca tctcctccat tatttccaac accacataca actgtgtaac tattatacat
    13021 gtctatatcc atatttttca ttgcagatat aaccgcattt tccatcaata caatcattgg
    13081 aatccctact tcttctacac atgcagtatc aacatttctg cttaatttag atgtacctat
    13141 tagcataaat tatcactcct ataaagttaa agacttctta aatacttacc aaaaatatct
    13201 attgaaatct ttttaatccc ataaatatca taaataaaat cactaaaact aagagttttg
    13261 aaataattta aaataacaaa aaattttgaa ttaaaacaat tatttaattt attataccat
    13321 ataatttaag ttattatgt
//
LOCUS       DAENCV010000102        13186 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAENCV010000102 DAENCV010000000
VERSION     DAENCV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.103"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            54..551
                     /locus_tag="KQD82_002887"
     CDS             54..551
                     /locus_tag="KQD82_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG4381345.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            1295..1657
                     /locus_tag="KQD82_002888"
     CDS             1295..1657
                     /locus_tag="KQD82_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG4381346.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            1722..2339
                     /locus_tag="KQD82_002889"
     CDS             1722..2339
                     /locus_tag="KQD82_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG4381347.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            2336..2821
                     /locus_tag="KQD82_002890"
     CDS             2336..2821
                     /locus_tag="KQD82_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG4381348.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2864..3382
                     /locus_tag="KQD82_002891"
     CDS             2864..3382
                     /locus_tag="KQD82_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG4381349.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMSLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            3518..4477
                     /locus_tag="KQD82_002892"
     CDS             3518..4477
                     /locus_tag="KQD82_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG4381350.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            4503..6206
                     /locus_tag="KQD82_002893"
     CDS             4503..6206
                     /locus_tag="KQD82_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG4381351.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            6419..8521
                     /locus_tag="KQD82_002894"
     CDS             6419..8521
                     /locus_tag="KQD82_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG4381352.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEDNEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            8534..8974
                     /locus_tag="KQD82_002895"
     CDS             8534..8974
                     /locus_tag="KQD82_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG4381353.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            9010..9813
                     /locus_tag="KQD82_002896"
     CDS             9010..9813
                     /locus_tag="KQD82_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG4381354.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9853..10449
                     /locus_tag="KQD82_002897"
     CDS             9853..10449
                     /locus_tag="KQD82_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG4381355.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAM
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            10555..11418
                     /locus_tag="KQD82_002898"
     CDS             10555..11418
                     /locus_tag="KQD82_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG4381356.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            11485..12474
                     /locus_tag="KQD82_002899"
     CDS             11485..12474
                     /locus_tag="KQD82_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG4381357.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            12566..13177
                     /locus_tag="KQD82_002900"
     CDS             12566..13177
                     /locus_tag="KQD82_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4381358.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5341 a         1270 c         2473 g         4102 t
ORIGIN      
        1 ttgaattagt tcaaaataaa atatattaat aaaaacaaag tgaggtataa agtatgaatt
       61 atgaaatagt agagataaat gagaaaatag ttataggaat atcgaaggag actacaaata
      121 aagatggaca agcggtaaat gatataggtg aattgtggaa aaaatttatg ggcaaaggga
      181 tatataatgc tataaagggt aaaaagaatg acaagacaat aggtctatat acagattacc
      241 agggtgattt tacaagtccg tatagctttg ttgcatgttg tgaagtaaat agtaattctg
      301 ataaagagaa gaatttagaa gaattaaaca ctaataatac tgttggagaa agtattatta
      361 gtaaagttat accagcaggt aagtatgcaa agtttgttat agtaggaggt caaaaagaag
      421 taggagattt ttggtttgag ttttggcaaa tggattttga taggacttat ataagtgatt
      481 ttgaagagta tcaatgtaat acttttgata caaagaaaca agaagttcat atttatatag
      541 gaataaagta gatttgttat tactatattg aatgaagtat tgagtatagt acatttatta
      601 tagatagttt tattatttat aactataagc agtaaatctg taaaagattt actgcttttt
      661 tagtaattaa tagtatcagt atcactttga atcttcagac atattcttca tttaatattg
      721 tttattgata aatgatactt gtaaatataa tgatttgtta tttagtttaa aatatgaaaa
      781 aaagtattat aaacacatta gaatacttct ggatttatgg gtatacaatt tgaaaaatat
      841 aaaaaataat gtataaagct aaatctaatt taatcgataa tacatataga gaaatataat
      901 taagtaaata aacaactatc tattagatat tgagaaagtc aaatcctata tgaatctaca
      961 gaaatgattt taatgaatct gaaaattttt tttatacaaa cataaaaaag gtataaagtt
     1021 gttttattat aatttaatta cttttgataa ctttttatgt tttatacaat tatattttta
     1081 ataactcgtc aaaacgccaa acatatatta caatagatat ataagttttt ttgttgacaa
     1141 attatgttaa gagaattaag tataatatgg cataaataat tacttttatt tatcaatgaa
     1201 atgtatttat tgtgtagcga aaaatagtca gatagtatag acaataactg aagataaaag
     1261 cgtttattgt tgtacatatg gaggagttag ttttatggaa aataaaattt tgatagtaga
     1321 tgatgctatt tttatgagaa tgataattaa agaagctctg agtaaaagtg gctataataa
     1381 tttaatagaa gcaaatgatg gagaagaagc gtgtaatata tttaaatatg aaaagcctga
     1441 tttgatgttg cttgatatta caatgccaaa aaaagatggt ttagaggtgc taagagaaat
     1501 aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct gcaataggac aagagaatat
     1561 gattgtagaa gccataaaac ttggagcgtt agattttata gtgaagccct tcaaaacaga
     1621 aacattagtt aaagttgtaa ataatgtttt aaaataaaag tgtaaatata cactaataca
     1681 atgaaaagta aataaatgca ataataaagg gaggggttta tattataaat tatttagaac
     1741 ttgatgatgt tcatatagat gctcttagag aaattggtaa tataggctct gggaatgcaa
     1801 ttactgcact tgcatccatg ataaatagca atgtagaagt attaatacct atggtcaaga
     1861 tacttgaata taatgaagcg acaaatttgc ttggtggtcc agaaaacaaa gtagtatgta
     1921 ttttactcga tatgaaaggt gatatcaatg gaatgtttat gtttttgttg gatgaatcaa
     1981 taactcaact tatgctaagt tctcttttta ataaagaaga agctttttta gatgagattg
     2041 aggcaattga aatttcagca attaaggaaa taggaaatat tatggcaagt tcatatgtaa
     2101 atgctattgc atctatgtta aacatgacta taagtgtttc aattccagat atatgtattg
     2161 atatggttgg agctgttcta aatgtgccta tgataagatt ttcagatgtt ggtgacaaag
     2221 tcttatttat agaaaataaa tttaaaatga gtgataatta ttttactagt cacattttga
     2281 tgattccaga aatgagctca cttagagaaa tactcgtgag gttgggatta gaagtatgaa
     2341 tagagagata gtagtgaaca ttgcagatat gaagatagcc tatagaccaa atgttttagt
     2401 tacttatgca cttggttctt gtgtaggagt atgtttgata gataaagtag ctggaattgg
     2461 tggaatgtta cacgttatgt tgccatatag taaagatgct attaacattg aaaataagtg
     2521 taaatttgct gacacaggaa taaatgagct cattaaatct atggagaata taggtgcaaa
     2581 tagaatatat ataaaagcaa aaattgctgg aggtgctcag atgtttttag gaagtagcaa
     2641 ttcagtaatt gcaagtatag gacataggaa tgttgaggca gtaaaaaaaa ctcttttagg
     2701 attaaatatt ccaatattag ctgaggacac aggtatgaat tatggtagaa caatacgttt
     2761 ttacactgag acgggtgaat tacttgtaga ttcaataaat aaaggtaaga agaaattata
     2821 atatatattt catagaaagt gtattttagg agggaggtat taaatgagat ttccagatga
     2881 cccatattta gattatggac taattgatga aggaattaag tatctaaagt ttaaagtaga
     2941 tgagcaagat tttggggttg aacttgaaaa ggtaatagaa attactggaa atcaagaaat
     3001 tatatttatt ccagaactac ctacatattc taaaggcata ataaatctaa gaggaaaaat
     3061 cattccgata atagatatga gtttgagact taaaaaagat gaattaatga ctacaaattg
     3121 catgtatata gtagtaactg aaataaagga cttggtagtt ggatttgtgg ttgatagagt
     3181 tgatgatata gtaagcttag aaaaaagtaa aatatcacct ccaccaagag ttactataga
     3241 atattcggat tattttgtaa gtggagttgg agaattagaa ggtaaaataa ttactttact
     3301 agatttagag aaacttttga cagataaaga tgaagtttta atagatgaat taatcaatgg
     3361 gtttgattgc actatcatgt aatataggga tgtagttgga gtgttattat aaaaagattg
     3421 tgatgagaac atttaagaat atagtggtat aattattaag tataaaaatt ttaaatcatt
     3481 aaaatttgaa tatattatgt ttaagagagg tttgtatatg aaaaataagc aacagggaac
     3541 aattttagtc gttgatgata gtgttctgat gtgtgactta atagataaaa cacttacaaa
     3601 agataatttt aaggttaaaa aagcatttaa tgcaatagag gcaaaagaat ttattgaaga
     3661 atttatacca gacattattt tgcttgatat aattttacca gataactctg gttttgagtt
     3721 ttgtaaggaa gtaaaatcta atgctcgtac agcagatata cctataattt ttataacttc
     3781 aaaaaataca gatacagaca tagtaaaagg atttggtgta ggagcaattg actatatggt
     3841 gaaacctttt agtatgacag aattaaaagc tcgtgttata gctcatcttg aagtaaagaa
     3901 atctcaagat aaattaaaaa agataaataa tgaactggaa tcttctcttg aaaaattgaa
     3961 tagactcgtt gtaagagatt atcttacagg tctttacaat aggcgacata taattaatca
     4021 gcttatggag caaagaagaa ttaataaata tagtgaaaca tcaatatccc ttatccttgg
     4081 tgatattgat gactttaagg ttatcaatga tacttatggg catgaagcag gggatttcgt
     4141 gttaacagta atatcaagta taataaaaaa gaattgtaga caaattgata ctgtatcaag
     4201 atggggtgga gaagagtttt tgataatgct cacaaatact ccaatagaag gtgctaagat
     4261 actttcagaa agaatccgta aagagataaa ggagtttgat ttcaactata aagaacataa
     4321 aataaagtgc actattacat taggagtagt agaagtaaac tcaaatatat cattagaaga
     4381 aaacatatcc ttagcagata aagcaatgta tgagggtaaa aacttaggaa aagattgtag
     4441 cgtagtgagt acaatgaaag gattaaaaaa gatataaact tactttatga aggagtacag
     4501 atatgaaaag agaagtaaag ctttctaaat tagcaattgt attgttgatt attatagtat
     4561 tggctgttct agcagggatt ttttcttcta tttcatctta taaagcattt gaaaaagtag
     4621 aaaaaagtat taatgatatg aataatttga ggactcaaag tcagtatata aaagatacaa
     4681 ctagagagat aagtgatgta ttaagaagat atgtatttac aggagaaaaa aagaataaag
     4741 atatttatga aaaagaatta tacggaaaaa caagagaatc agctttaaac aaattagaaa
     4801 aaattggtct tacaaagagc gagtcaaata aaatcttaga agcaaaagaa atatcagatt
     4861 ctttagtacc aacagaacaa agtgcaataa aggcagtcga aagtaatgat aagagtaaag
     4921 ctcaaagtat aatttttggt agtgactata taaactccca agagaaaatg gattctttaa
     4981 taaatgaatt tcaagaggat atagttaccc gttcaaattc caaaattgta gaagtaaaaa
     5041 aagaattaca aagtctaata ttaagaacaa tatttatact ttccctttta ataatattga
     5101 caataataga atatacagta ataaaattaa aaatagtgat tcctattaat aaagtagaaa
     5161 aacacttttc aagaattgca tctggggact taagaacaag tatagacgtt gaagaaagta
     5221 aatctgaaat agggctatta gtgagttctg tcaaaaagat gcaaagtatg cttttaaatt
     5281 acatagacct tatagacagt acattaacta gtattgcaaa aggagattta agagtagaga
     5341 tagaccaaga atttataggt gattttaaat caattaaaat atctttagaa agtataataa
     5401 actcttttaa tggtgatttt tctgaaataa atttatcagc agagcaagtt gcaagtgctt
     5461 cagaacaagt tgcagcaggt gcacaagctc tttcacaggg tgctactgaa caagctagtt
     5521 cagtagaaga attagcttcc acgattaatg agatatcaga tggaattaag aagtgtgcag
     5581 atagtgctag tcttgcatgt gaggtatctg accaatctgc atcacaagtt ggtttcggaa
     5641 gtagatgtat ggatgatatg atgacatcaa tgggtgaaat aagtgaaaaa tcgagtgaaa
     5701 tatcaaaaat aatcaaaaca atagatgaga tagcattcca aactaatatt cttgcattaa
     5761 atgcagctgt tgaagcagca agagcaggtc aatatggtag aggatttgca gttgtagcag
     5821 atgaagttag gtctttggct ggtaaaagtg caaaagcagc tcaaaataca gctattctca
     5881 tagaagaagc tattaaaaca gtagaaaatg gtgtcagaat tgctaaagag actgcaaatg
     5941 cattggactt ggtagtaaat ggtgtagaaa aaactactaa ttatattaat ggtatttctt
     6001 cggctgtaca tagtcaagaa gtagcaatta cacagatttt atctggaata gagcaaattt
     6061 ccatagtagt ccaaatgaac tcagcaacag cagaggaaag tgcagcagct tcagaagaga
     6121 tgtctgctca agctcaaatt ttaaaagaat tagtagaaaa atttcatcta aaggaaagtg
     6181 aagtaagaga atcatcatgg gtttaaatta tgaggtgaat attgaagtaa gagaattatc
     6241 gtgtatttaa attatgagat gtatatatat tgaaataaga gaatgattgt gtatttaaat
     6301 tatgaggtac atatagtgag ataagagaat catcaaggat ttaaattatg agatgcatat
     6361 ataatagaaa attatttgta ggtcgtaggt gataaatgtg aattatattg aaccgagtat
     6421 gagacaaatg ttagatatgt ttatatttga gacaagttta ttactcgaac aattagatga
     6481 aattatgatt caaatagaaa aagagaaagt atttactcat aacaacatta atgaaacttt
     6541 tagaataatg catacgataa aaggttcagc atctatgatg gggttagaca atatatctgc
     6601 tttagcacat agtgtagaag acttgtttta tgctatacgt gaaggtaaaa tagaagtttt
     6661 agacaatgac ctattatttg atttagtctt tcaatcatta gattttataa gaagagatat
     6721 agatgagtta caatcaagag gttgtatatc ttctgattac tctaatttta ttgaaggaat
     6781 tgaagagtat gtgagtactt tggaattatc aagttctatg aaatcaaagg ataatgcaaa
     6841 tttagaaagt aaagacgaaa aagaagtaat agattcaaat tttgaataca aagaagactc
     6901 tgatgtagta aaaataatct ttgaacaagg ttgcatgatg gaaaatgtga gagctttcat
     6961 gcttgtattt gaacttagag attactgtga atttatagat ttttatccaa aagatgtaga
     7021 aacaaatgca gagtctacag agtatattaa aagttatggg ttttatatac aatttgtagc
     7081 taaagaggat aaggcaatag tttataaaac tatagaagat aatctctatg taaaagaata
     7141 cataaatatt agtaaaaaag aaatagaaat gaaaaataca tcaaacaatg atgcagtaga
     7201 aaataaatta aaagataata ataaagtaag taaaagttat caaaataact atacagaaga
     7261 tgagatacaa gaagatgttt taaatgaaaa aactgatatg gaaaaagata gtctaagtga
     7321 agacaatgaa catttaccaa taagtaagca gagtattatg agtgtaaatt tagaaaaatt
     7381 ggacttactt caaaatatag ttggagaaat tgtaattatg gaatcaatgg ttgtaaatag
     7441 cgtaaattct acaggtgttg atatagataa tttcaataag gcaacaagac aattacacaa
     7501 gcttacagat gaattgcaag atatagtaat gtctatgcgt atgattcaaa ttgctggtgt
     7561 atttcaaaaa atgcatagaa ttgtacgaga tatgaataaa aaattagata aaaatgtaga
     7621 gttaataact ataggagcag agacagaggt agataagagt gtaattgata atttagcaga
     7681 accctttatg caccttataa gaaatgctat ggaccatgca atagagccta ttgaggagcg
     7741 tatagcgaaa ggtaaagaag aagtgggaaa gatagtttta tctgctgaaa atctaggcgg
     7801 agaagctgtg ataagtattt cagatgatgg tagaggcatt atgagagata agattttggc
     7861 aaaagctaag gaacaaggtc ttcttaaaca tcaaagtaat gagtatactg atgacgaggt
     7921 caattctttg attatgactc caggcttttc tacaaaagaa tctataactg aattttctgg
     7981 aagaggagta ggcatggaca ttgttcgtaa aagtattgaa aaagttggag gttcaattga
     8041 agtaaaaagt aaacaggatg aggggacaac ctttactata aaaataccat taactctttc
     8101 tatagtagag ggtatgaagt ttaaagttgg agattcacta tttactttac caatatcatc
     8161 cataagaaga acatttaaac ttacagactt aaatcaaata attacagaag gaaataagac
     8221 agagttaatt acaatatatg gagcatgtat tcccattata aggttacata aaatatataa
     8281 tattcaaaca aaagtaacca atctgatgaa tggtaatatg ataattattg aaaatgagca
     8341 taaagcagta tgcatttttg ttgatgaaat aataggtaat cagcaagttg ttgtgaaacc
     8401 atttccagtt tattttaacc gatttaatat aaaaaataag ggtttatcgg gatgtacaat
     8461 acttggggat ggaagtataa gtttaattat agatgcagat agcattatag aaatatatta
     8521 ggggatttaa agtatggcta aagatgaaat tattaaacaa gtattaacat tttatgtcaa
     8581 tgatgttatt tatggaatag aattagaaaa tgtgattgaa accataagat ttcaatcaat
     8641 aacgtatgta ccatgtcttc caacatatat aagtggagtt attaatttga gaggaagaat
     8701 aataccagtt attaatatgc atataaagta caatcttcct aaagctgatt ataatgagag
     8761 aacttgtata ataattacaa aggttgatga gtatcaagtt ggaattattg tagataaagt
     8821 tgttgatgtt attcatatag aaaatctaaa tttactagag acgactaatt ctaacaacac
     8881 taatattaat aaagatataa aagagattgc gaaagttgaa gataattcta tattaatttt
     8941 agatattcga aagtttttga ttaatagcat atagttagtt ctgataaatt agcaggagga
     9001 agtttttgca tgataaaact cacagatgaa gaatttatag ttcttgtaaa ccatgtaaaa
     9061 aaagaatatg gtattgattt aagtaaaaaa agagcgttaa ttgaaggtcg cttatacaat
     9121 acaatgatag agaaaaaact tagttctttt agtcaatata tgaatctatt atttaaagat
     9181 aagacaggaa atgaagctat aaacctaata aatagattga gtactaacca cacatttttt
     9241 atgagagaac ctcaacattt tgagtttata cagaatagta tacttccttt ttgggaagaa
     9301 aataataaaa taagaaacat aaatatatgg agtgcaggat gttctagtgg agaagaagcg
     9361 tattcaatag ctatgacttt ggatgattat tttggatata ataaagagtt gtggaacatt
     9421 aaaatcatag ccacagatat ttcaactaac tcacttgaaa aagctaaaag aggtatatac
     9481 atagaatcaa atgttaacaa tgttcctgag ttatggaaga agaagtattt tatagacaac
     9541 aaggatggta catttaaaat ttgtgataag attagaaaaa gtgtaatttt taagccgttt
     9601 aatttaatga atgatttctc atatcaacat tttgatttga ttttttgtag aaatgttatg
     9661 atttattttg atttaaaaac aagagaagaa cttataaata aattttataa tgctaccaaa
     9721 aaagatagtt ttttgtttgt aggacatgca gaagtaatca acagaagtag tactaaatac
     9781 aaatatgtaa aacctgctat atataaacga taaaaaggta tttttgatat atgatttttt
     9841 ggaggcggta atgtggaacg ttttaataat atggttattg ctataggcgc atctgtaggt
     9901 ggtacagagg caatacttga aattataaaa gacctaccaa aatcaacacc agggattgtt
     9961 gtagttcaac atatgccagc tatgtttact tatatgtatg cacaacggct tgacaaacag
    10021 tgtattatga atgtaagaga agctaagaat aatgatagag tagaacaagg aaatgtgtta
    10081 attgcacctg ggggttacca gatgaaattg tgtacggata aacaaggata ttatgtaact
    10141 tgtgaaaaag gagaaagaat aagtggtcac tgtccatcag tagacgtttt gtttgattca
    10201 gtagcagaag tagcaggtaa aaattcaata ggaataatac ttacaggtat gggttatgat
    10261 ggagcaagtg gacttttaaa aatgaaagaa aatggttcat ttacaattgg acaggatgaa
    10321 aattcttgta tagtatatgg aatgccaatg gtagcttttg atagaggttc tgttatgtta
    10381 caattacctc ttggtgaaat ttcaaactgt ttgataagac atattaatat tttaaaacaa
    10441 aaagattaat tttttataaa taaaaattaa tcttttatta aattatatgg ataaaaaacc
    10501 ctagaaactc caaatataat aagagagatt tatatttagg aggaaaatga taatatgaaa
    10561 ttttttaaag tcgatacaaa agctaaagtt ttaagatatg tatttgtttt aacattgact
    10621 ataatggctg ttttggcaat taaacatgac tataaatata ataatttaag gaaagatgct
    10681 tcaattaatt taaatagtaa taaggaagca gtgactgtta tgagtaacca gtcattacca
    10741 aagggtaata caaatagtaa acaaacttta tcagtaagta caataggaag taaaacaata
    10801 gtgaaaaatg atgagtattt agaaagtact ataaacatgc ctgtaataac aaattcaaat
    10861 aaaattgttg aaaggtctac aaatgataag ataaaaaatg acatatttga attttataat
    10921 aaatcatata aagaagcaaa gcagtatctt aaggataatc ctgacgagaa aaataagttt
    10981 gttgcaaatg tagactttga acttaaaaag aatactgatt ctgctcttag tataaaagtg
    11041 agatattaca cttatagtgg tggagcacat ggattttatc aagatatagc atataatgtg
    11101 gatatgagaa ctggaaaatt tttagaatta atggacttgt ttaaggataa tactaaatat
    11161 aaagaagtta tagatgaaga aattaaaaga caaatagctg aacttgaaaa aaaggatgaa
    11221 gaaaatatcg gaatatataa ttttaaaggt ataaaagaga atcaaaactt ttacttgcaa
    11281 gatgagaatc tagttgtata ttttgattta tatgatataa ctccatatgc agcaggaata
    11341 ccagaatttt caataaataa agcgttgata agttctatgc taaaaccaga gtatgtagat
    11401 ttatttgatt tgaaatagaa gattaaatta aatgttatgc tataatattt agcatagaga
    11461 cttattaaaa ttgggggtat aaaaatgcta cttttaaaaa aagacgacat aaaaaaagta
    11521 tttacaatga gagatgcaat tgaagctgat aaggaagctt ttagaattta ttgtgaagga
    11581 aagagtgtaa atcctttgag aaccaatata tctgttccaa gtcaagatgc tagtatgtta
    11641 tttatgccag gatacgtaga agaattaggc tgtgggggaa taaaaattgt atctgtattt
    11701 cctaaaaatg ctcaaaaagg caaaccagta attccagcaa cagtactatt attagatgga
    11761 gaaactggag aagttagtgc agtattggat ggtacttatg taactcagat tcgtactgga
    11821 gcagcctctg gagcagctat agacgtattg gctaaaaaag attctaagat tggagctttg
    11881 ataggtctag gaggacaagg tgagccacaa cttgaagcca tgatagaagc tagaaatttg
    11941 gatgaggtaa gagtttttag tagaaacaag gaaagtagag aaaaatttgc agaagaaatg
    12001 aacttaaaat taagtaaata taatactaaa atagttgcag ttgaatcatc tgatgaagcc
    12061 atagataacg cagatgtgat agttttagct actccatcaa aacaaccagt acttaatgga
    12121 aacttagtga aaaaaggagc tttaataagt gcagtaggtt catatatgcc agatatgcaa
    12181 gagttgtgcc caaactgttt aactagagcg tctaagatat tttttgaatc aacagatgca
    12241 gttttatctg agtcaggaga tatattaatt ccattaaaag aaggtaagat aagtaaagat
    12301 gattttagtg gtgatttagg gaatgttata aacggaacta ttcctggaag agaagatgat
    12361 gatgagataa ttgtatttaa aactgttggg ataggtgtac aagatgtcgt aacagcaaaa
    12421 agaatatacg ataaagcaaa agaaaacggt attggaatag actggaagtg gtaaaatatt
    12481 caaaataatt ctatagttag acaaatttat gttataatgt tgataaataa attttttatt
    12541 attttaattg agggaggatg tttgtatgaa aatggtaaaa aagttaatat tatctacgat
    12601 tatggctcta gttttagttc taactatagg gtgttctaat gcagaagaaa aagtaaagtc
    12661 gaattttgaa gctaatttag ctgcattaga aatgtataag tatgatgaaa aaggaatttc
    12721 tgatgcatta acagaagctg aatataaaca gattcaaact tataacagca aggacaaggt
    12781 ttcaatgatt gctcaattga cacctcttta tggtaaagaa aatgctgaaa agatatacga
    12841 aacaattgtt aaattaattc aaggtgttga aaagacagta acagtggaat cttctagtaa
    12901 ggataagata actttgaaag ttgtaagtaa agctttagat actagtaaag taatgagtga
    12961 agcgaacact ttagtaaaat cttatgtaac tgatgaaaag aattacgata aaaaagaaaa
    13021 agaagttcaa gatgatatga caaaaatgat tatagacctt tacaataaga aaccaactaa
    13081 ggaagttaca actatgatta catatactaa aaaagatggt aaatggcaac cacctagtaa
    13141 tatacaaaag ttatgtgaag gaatatatac aatttaattt atttta
//
LOCUS       DAENCV010000103        12873 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAENCV010000103 DAENCV010000000
VERSION     DAENCV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.104"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     regulatory      93..195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            289..1665
                     /locus_tag="KQD82_002901"
     CDS             289..1665
                     /locus_tag="KQD82_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG4381359.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1779..2924)
                     /locus_tag="KQD82_002902"
     CDS             complement(1779..2924)
                     /locus_tag="KQD82_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG4381360.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3177..3884
                     /locus_tag="KQD82_002903"
     CDS             3177..3884
                     /locus_tag="KQD82_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381361.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3931..5526
                     /locus_tag="KQD82_002904"
     CDS             3931..5526
                     /locus_tag="KQD82_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381362.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKVLMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5629..6711
                     /locus_tag="KQD82_002905"
     CDS             5629..6711
                     /locus_tag="KQD82_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG4381363.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6860..7021
                     /locus_tag="KQD82_002906"
     CDS             6860..7021
                     /locus_tag="KQD82_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG4381364.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7362..8630
                     /locus_tag="KQD82_002907"
     CDS             7362..8630
                     /locus_tag="KQD82_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG4381365.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8825..9613
                     /locus_tag="KQD82_002908"
     CDS             8825..9613
                     /locus_tag="KQD82_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG4381366.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9613..10062
                     /locus_tag="KQD82_002909"
     CDS             9613..10062
                     /locus_tag="KQD82_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG4381367.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10066..12330
                     /locus_tag="KQD82_002910"
     CDS             10066..12330
                     /locus_tag="KQD82_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4381368.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIETSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12470..12781
                     /locus_tag="KQD82_002911"
     CDS             12470..12781
                     /locus_tag="KQD82_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381369.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4718 a         1403 c         2193 g         4559 t
ORIGIN      
        1 tataatcgaa tattaataca aaatcaatac aatatatacg tatatattga caaaattaat
       61 tatataaatt aaaataatat tcgtaaagaa aaattcatgt tttgtactgt ataacatata
      121 attttgacaa tatgggtcat aagtttctac cggaataccg taaatattct gactatgtat
      181 actattattt agttaattaa atatttaatt aactgaatta tgaaggatac ggctcagctt
      241 atcaactact gtgtcgtttt tttgtaaaat ttaataggag gtgttgttat gcaaacaaca
      301 aacagtagta ctatgcagaa actttttcca attttgacta ataaaaatgt cgacatgaag
      361 aaggaaataa ttgcaggagt aactactttt ttaactatgg cttatataat agctgtaaat
      421 ccaaatattt tatctgagac aggcatgcct gcaggtgcac ttgttacagg tacttgttta
      481 gcagcagcat ttggatgtat tttaatgggt gtagttgcta accttccttt tgctttagca
      541 tcaggtatgg gtcttaatgc attttttgca tatacagtag ttttacaaat gggtgttcct
      601 tgggaagtag ctcttacagc agtatttgta gaaggtatta tttttatagt attatcagta
      661 agtggagttc gtgaggctgt tgtaaatgcg ataccaaaaa atatgaaatt agctgttaca
      721 ggtggtatag gaatatttat tgcacttata ggtctagtta acagtggaat tgttattgga
      781 gaccaagcca ctcttatcaa aatgggtaga tttacaccag cagtaataat aacttgtgta
      841 ggattaataa ttattgctgt attagataaa aaaagagtta aaggttctat actttttgga
      901 atagtagtga gttcattact tgcttgggca tttgctttta taaatccaga gtatgcacaa
      961 aaattaggta tatatttacc tggtggatta tttaaattcg aaagtttagc accaatagca
     1021 ggtaaaatag atttaggata tgtactacat ccaacaaata taggtggttt tttagtagta
     1081 gtttgtacat ttttatttgt tgattttttt gatacagtag gtacattagt tggtgtttgt
     1141 tctaaagcaa acatgttaga tgaaaaaggg aatgttccta atgtaggtag agccttactt
     1201 acagatgcgg ttgcaactac agttggtgca ggacttggag tatctacagt tacaacttat
     1261 gttgagagtt caacaggagt tatcgcaggt ggaagaactg gatggacagc aataacagtt
     1321 ggaattttat tcttagctgc aatgtttttc tctcctgtat ttatagcaat tccatcatgt
     1381 gctacagcac cagctttaat atatgttgga tatttaatgc ttggtacagt taaggatata
     1441 gagtttgata atataactga aggtgttcca gcttttgtta ctattgcttg tatggcatta
     1501 acatatagta taggtgatgg tttaacttta ggtatattaa cttatgtttt cgtaaatata
     1561 ttttataata tatttggagc taaaaaggca gaagacaaaa aacatgtttc tgtagtaatg
     1621 attgttctgg caatattatt tataataaaa ctttggctac tataataggt attatttaaa
     1681 attttaattt aatataaaat aaagggctgt aatttattat caattgtgat gataaactac
     1741 agctcttttg tatttgttgt atttttgtat ttattgtatt atttaataat gtctattagt
     1801 tctccatttt gtaaagtttt aggtgtaatt actatattga actctttttg caaatctttt
     1861 ataatatcta attctttttt tgttattatt gagattgtgt taccacttcg atttcctcta
     1921 gcagttcgac cacatctatg caggtattca tttttacttt ttggaaaatc caagttaaaa
     1981 acatgactta catccacaat atctagtcca cgagcagata aatcagttgt aattaaaatc
     2041 tttgctttac caagtttaaa tttatttatt gcattctttc tatcttcttt ttccatattt
     2101 ccaaaaattc ctattgcttt gtaattatga taatttagtt ttgatactag gacttctata
     2161 tttttttcat tatttacaaa tacaatagct ttttttggat ttgtagctgc taatgccttt
     2221 ctaagaaaag taaatttatc ccttatctca cctaataaat atgagtgatt gatgttagga
     2281 tttatttgtg atttttcctt tgtttttatt atttcaaact ccttcataag tttatcacaa
     2341 atttttatag tgctatcagt aagacttgca gagcatccta taatttgtct atctcttaac
     2401 gttgttctta ttatgtcttc tatacaagtt atattttttc cattcaatag attgtccact
     2461 tcatctaaga taatagtttt tatattatgt gattttagtt tcttttgttt tatcaagtct
     2521 aaaactcttc cacaagagcc aattactatg tgtggtttta ctgcttttat attttttatt
     2581 tgtttttgta tattgacctc cccaattaaa gctaaagaag taacggagag ttctgcattt
     2641 ttagccaata attcaacctg gtttgtaatc tgcattacta attcatgcgt aggtgcaagt
     2701 ataagagctt gtgtttctct ttttgatgta tcaatttttt caaatatagg taaaaggtat
     2761 gctagtgttt ttcctgttcc agtctgtgaa ttaatcaata aatccttatt ttgaattata
     2821 ttccctataa caagtgactg aacttctgtt ggagaagtta tgtcttgttt tttgagtcca
     2881 tctattagag tggaacttat ttttaattgt tcaaatgtat tcatatttta ctccttatat
     2941 tttaataatt atagtcaact attttttatt atacattgtt tattcaaatc gaactatata
     3001 ttcttttata attatgtgta atttgaaagt aaatatcaat tatatttata cctttatatt
     3061 tctgatacct ttgcatttta tagatttcta aaataaacaa ataaaagttg taattgtttg
     3121 atatttgtta tatattatat ataacaaata tcaaaactat aatatggagg gaaattatgt
     3181 taaaagtaat caatgtatct aaatcatata aaaataaaaa agtagtaagt agtgtctcat
     3241 ttgatgtaaa tgatggagag ctttttgggt ttataggaca taatggagca ggtaaaacta
     3301 ctactattaa agcaattgtt ggaatacacg attttgaaga gggagaggta ctaataaatt
     3361 ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga aatggcatat atacctgata
     3421 atccagattt atacgagtca ttaactggta tacaatacct taattttata gctgacatat
     3481 ttgaagttgc caaagataaa agagaagagt taataaaata ttattcaaat aagtttgaac
     3541 tcaataaagc attaggagat ttaatatctt catattcaca tggtatgaaa caaaaattag
     3601 ctataatttc ggctttgata cacagcccaa aaatcttaat tcttgatgaa ccttttgtag
     3661 gtcttgaccc aaaagcttcc tttatattga aggaaattat gagggagttt tgtgacaaag
     3721 gtggttgtat atttttctca acacacgttt tagaagttgc tgaaaaaatt tgtgataaga
     3781 ttgccataat aaaagatgga aatataattg cttatggaac tacagaagaa gttaagggaa
     3841 gtaattcact tgaaagtatt ttcatggaat taatagagaa ataaaatgtt ttttatacaa
     3901 tatagtgtct aaagtgagga atgataaaat atgagcaatc taagtatatt aataaaaaat
     3961 aatataataa atgaatttaa attaaatgcg ttaaaaaaag ctgacggtag agaaaaacaa
     4021 aaaataatgg gaatgatgct ttcagttgtt ataatagcgg gtctattaat tttttatgta
     4081 acgagcttaa gtctctcttt agttgatatc ttaaagcaaa taaatcaact ggaaatgcta
     4141 ctgatatttg gttttggatt atcaacatta acaactattg ttacatcatt atataaaacg
     4201 tcatcatatc tatttcaagc aaaagattta gaccttttga ctagtttacc tataaaagaa
     4261 tctactgtac tagcaagtaa gatattaatg ttggttggag caaactattt attttcttca
     4321 atatgtatga ttatacctgc agtggtttac tttttaaatt ctgatgtaag tataacttat
     4381 ttcccttatt tgatattgct atttttagct ttaccattat taccgatagt tgcttcatca
     4441 ataatttttc tatttatagg gcagatatca tcaagaataa aatacaaaaa tttagtatta
     4501 ataataggta gcattatatt gatgttgctt tataccctac ttatgtcaag acttaattct
     4561 atgtcagttg aaatgctaaa gaatagtgtg tctatttcag agacgattag aaaagtatat
     4621 cctcctatat attattttgt tgatgctctt aaaacaggaa gtatagtttc atttttagca
     4681 tttatagcca tctcaataat accatttttt atgtttgtaa ctctgtttgc aaaaaaattt
     4741 aaagatataa atgctaagat gagtgaagga tataagtcaa aaaattatga atttaagggt
     4801 caaaaaactt caagaccttc aaaagcttta ttaaaaaaag aatttaagag atatacttct
     4861 tcgtttatat actttttaaa ttcatctgca ggtttgttat ttctaatagt aggtacaatt
     4921 ttaatattat tatttggagc agataaagtg ggtgagttac tgaaattgaa tatagatttt
     4981 aatcttataa aaatacaatt attgggagta ttcttcttta taatagtaat gaattgtaca
     5041 acatactgtt caatttctct tgaaggaaaa aatttatgga tactgaaatc ttctccaata
     5101 gatgaaagag aaatattctg gtcaaaagta ttaatgaatt ttttattgaa tgccccatta
     5161 tctattttat gtctaatatt agtatctata agattagact ttgaaattca gtttattatt
     5221 gtatcaatct gtttgattgt aagtttttca atgtttgtag cattgatggg attattatca
     5281 aatttattat acccaaacct tgattggaaa aatgaggtgg ctgtagtaaa aagaagtgca
     5341 agtatgatag ttacaatgtt agtatcatta gcttatgtaa ttatacttgg aggtatatat
     5401 gtattacttg gaatttcgtt tttaaatata tatattattt tggcaagtat acttactttg
     5461 atattagatt ttatgatatg gagaattatt tctactaaag gagtaagaat atttaaaaat
     5521 ttataggtgt atttatgtca aataaagact tatctttttt agtcttgcaa ataataggtt
     5581 aagtagtata ctatgtatag tgatatgaaa aaatttagga gaaaataaat gagaaaatta
     5641 tccaaaagag aaaaagattt tattcaatat ggatttttat tatttttgat atgtattact
     5701 acttatttag tattaactac tttagatata aaactaatac ctaaaattat aaggctagta
     5761 aataagactt atatatttct tggcatattg acaattatag tatatatgat aattgaatct
     5821 attgtaactc atttgataat aaatgcagtt caaaaaacaa aagtaaaatt tattggtatt
     5881 aaaatggcta caatgggatt ttactataac ttagttactc catttgcatc tgggagtcaa
     5941 ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc ttagtaaatc agtggctata
     6001 gttactaata aaactgtagt atttcaaagt acagttactg tattttgtgg aattttaatt
     6061 ttgctaaata agtcattact gatggagcaa gtacattctg tgaaagtctt gataactttt
     6121 ggaatgatta tgaatgtaag tatgttgtta ggtggactac taatagtatt tagtccaaaa
     6181 caagtaaaaa tgatagcaga agttttgatt aattttttat ctaaatttaa aacatttaaa
     6241 tttttagatt caaaacgtga tagaataaat cattatattg atgattataa ttattctata
     6301 aaaatattta ttaaaaatgt aagaatctta gttttaagta tattacttac tgtgttacaa
     6361 ttaatcgcat atttcagtat agtttattgt gtgtataaag cctcaaattt aagaggagtt
     6421 tcatatgtac atataatgac attacaggtc tttttatata tggctatatc accaattcca
     6481 acaccaggaa atgttggagc taatgaggtt acatttttta ctatgttctc caatataatt
     6541 ccaaaagaat tattggggta ctctgtactc ttatatagtg ggtttgtata ctattttata
     6601 ttaatagttt caggtttatt tacatttgga acgcattata cattaaattc ttggaaaaag
     6661 gaagataaag taaattctaa tttagataag gttattgaaa aattaaatta ataaaataat
     6721 tagggaaatt ctatatataa atatggtgtt gaataagcta tattatattg agatatttcc
     6781 cttttttgat taaaattata gaaatttgaa ataataagta ataaacagaa aaagtgatat
     6841 tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat gaaaaacaaa gtgagatttc
     6901 taagataaaa caaagcaatg ataataaact tgaaatagct caagaattac atgtaagtaa
     6961 aaatataggg aatatgcttg gatatagaat agataagaaa aaaatgaata gaccaagtta
     7021 atgaaaatgt tgaaaatggt acattttaaa aaatatatat aatgaaatgt tttattgtta
     7081 aatttaaact tattttaatg gaggaaaaag tcgaaaaata tctaaaaaga tatggatata
     7141 aaaaatatta atatatatat actataaact tgacaaaatc aaccttttaa taactagcaa
     7201 aaaatagcaa aattatataa gcgttattta attgtcaaaa atgaaagcta aattgcatgt
     7261 cctaaaaaaa atgattgata acatttattt gtgtgctata attatatagt aaaacaataa
     7321 gtatattgca taatgtatgc aaatcgggag gagagacgga catggaagca tctatacaca
     7381 caattgccag caataattta ttgataattt ttgctattgt aagtataact ggaataattt
     7441 gtagtaagct tagtgaaata ataaaggttc cagatgtagt gttatattta cttgtaggta
     7501 ttttaatagg accatcattt ttaaaattta tagatattcg tggatttcaa atagaaaatc
     7561 aactaatact tacatttggt tctgcattta ttttatattt aggtggaaaa gaaataagtt
     7621 taaaagtatt gagaaatgtg aagatatctg tattcttatt agctacttta ggagttgtga
     7681 tatctgcttt tataatgcaa caagtaatag gatttacttt tgggataagt gcaatgactg
     7741 ctttactagc aggaactata atagcatcaa cagacccagc cactcttgta cctatattta
     7801 atcaggtaaa gataaaggat agggtaaaac aaactgtaat aagtgaatca gcttttaatg
     7861 atgcaacagg agctatacta acatcagcag tgttggctgt aattttgtca aataaatttt
     7921 cattaggtga aaatatttat gaactgggga caatggttat tgtcggagtt ttagttggtt
     7981 taattacagg aatactgctt ttgaaattag ttaatgataa accttatgga atatttaaag
     8041 attttgcacc aataatatct gttatttcag ttgtagttgc atatgaatta tctacaaaat
     8101 taggtggaag tggatatatg tcttgcttta ttgtaggtat aataactggt aacaagaaaa
     8161 actttaaaat ttggttaagt caaaaatctt atgatgcaga tttttatgtt gctgaaactt
     8221 taggtactat ttgtcgtatg gcaatattta taatattggg tagccaagtt aatttggctc
     8281 agttaagtaa atattttatg ccatctgtaa ttacagtatt agtattaatg tttatagcaa
     8341 gaccactttg cgttttagtt tgtacacttg tagacaggga agcaaaatgg agtaaaagtg
     8401 aaatattatt tatgatgtgg gtaagggaaa caggcgtaat accagcagca ctttgtggta
     8461 ttatttcagc aatgaaagtt cctggatacg aagtgatttc atctgtagta ttcatgacta
     8521 tactaattac attagtaata caaggaagta ctacaaaatt agtagctaaa aaacttggat
     8581 tattagaaga agaagtgata aatataagtg aaaaagtatc aactttttaa agttttgctt
     8641 taaaatagta cctgttaatg gctattttaa tattgtctaa tgatgattat tgaaatagtc
     8701 atttttttat gtataagtaa tttacagaat ttacatatta gacatattta ttatcaaaat
     8761 ttgttgaagc tatgatataa ttagttgtat ttaaaataag aatagcaaat aagggaggaa
     8821 atttatgttt acggttatgg atattgtaca gccagataca gtagaagaag cgtactcaat
     8881 tttaaataaa agaaaaacta accaagtaat tggtggaagt gcttttttaa gaatggggaa
     8941 aaaaagaata ggaacaggaa tagaattatc taaattaaat ttagattata taaaagagta
     9001 tgaagattac gttgagattg gttctatgac tacttttaga acattagaaa caagttccat
     9061 aataaaaaat aattttggaa gaataataga agattcagtt aaaaatataa taggagttca
     9121 gtttagaaat gtagtaacag ttggtgcaac agtattttct aaatatggtt tttctgactt
     9181 gatagtagca ttactttcat tagatacaga agtagaatta tataatattg ggagaattag
     9241 tcttgaagag tttctaaata gagattatga gaaagattta cttattaaga tatacataaa
     9301 gaagacaaat aagaatgctt cttataaatc acttagaaat gcaaagagtg attatccaat
     9361 tctaaatgta tctgtatcaa aacatatgga acaatttaag ttatgtgtag gagcaagacc
     9421 gcagaaagct acgattgcaa aacaagctag tgaatttcta tctaacaatg aaatcaatga
     9481 aattaatata gataaagcag tagaaatagc atcagaagaa ctaacttttg gttctaacat
     9541 gagagcatct agggagtata gaaaagctat gagtaaagta cttttaaaaa gagctataat
     9601 ggaggttatt taatgttagt aaagattaat ataaatggga agaaaaaaag tgttgatata
     9661 gaaccagaag aatatctggt tgatacttta agaaaagttg gtaatttaag tgtgaaaaga
     9721 ggttgtgaca caggctgttg tggtttatgt acagttttaa ttgacaagaa acctacatta
     9781 tcctgtgcaa cactgacatt gagagcttta gataaagaaa taactacaat tgaaggttta
     9841 gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag aaggagctga gcaatgtggt
     9901 ttctgttctc ctggctttat acttactgta cttgcaatga aggatgagct tgaaaatcct
     9961 acagatgatg aaataaagca ttatcttaca ggaaatctat gtagatgtac agggtatatg
    10021 ggacagttaa gagctatcaa aacttatctg gaggtaaaat agaacatgaa tatagttgga
    10081 aaaggaatca agaaaataga tggaatgagt atcacaacag gaaagcctgt atatacagat
    10141 gatttatcag caaaagatgc tttggtggta aagatactta gaagtcctca cgcttatgca
    10201 aaaataaaag atatagagac atcaaaggca gaaatggttg atggtgttga atgtgtttta
    10261 acatataaag atgtaccaaa atctcgattt acacttgctg gtcaaacata tccagagcca
    10321 tctccatatg atagattaat tcttgaagat gttgtaagat atgtaggtga cgaagtagct
    10381 atagtggcag ctatagatga aaaaactgca attaaagcaa tgaatcttat caaagtaaag
    10441 tatgaagtac ttacacctgt tttggatttt gaagaggcaa taggaaataa aactatggta
    10501 catgatgaag atagacattg taactttgat ataggtatgc aaagagaaaa aaatatagta
    10561 tcccatcatg attatacaaa aggtgatgta gaaaaagtat taagtaattg tgatgtagtg
    10621 attgaagaaa cttattatac tcaagctcaa gcacatgcaa tgatggagac gtatagggca
    10681 tacaattatt tagaccatat gggaagattg gttgtagtaa gttcaactca aataccattt
    10741 catgtaaaaa gacatttatc tcgtgcacta gaaataccag ctagcaaaat tagggttata
    10801 aaacctagaa ttggtggcgg atttggtggt aaacaaactg catgtgttga gattttttct
    10861 gcaattgtta ctttaaaaac agggaaacca gctaaaataa tttatgatag aaaagaaact
    10921 tttaattgtt caacaagtag acatgcaatg agattaaaag ttagactagg tgctacaaat
    10981 gatggcataa taaaggcaat agatataaaa gcactttctg atacaggagc atatggagag
    11041 catgcttcta caactattgg tttggttgga gaaaaaactt taccaatgta taataaagtt
    11101 gaagcaacaa gatttattgc agatgtggta tatactaata agatgccagc aggagcactt
    11161 agagggtatg gagcatcaca aggatgtttt gctgttgaat caactataaa tatgcttgct
    11221 aataaattaa aaatggaccc aacagattta aggttaaaaa atgtggtaac agaaggtgaa
    11281 actacatttg catatggtaa aactttaaat agtgtcgatt taataaaatg tataaataaa
    11341 ggcaaagaac ttattggatg gaatgaaaaa tatccttgta aacatcttga taatggaaaa
    11401 attagaagtg tgggtatggc aattactatg caaggttctg gtatacaagg tcttgataag
    11461 tcttcagctg aaataaaatt gaatgactgt ggagattata ctcttatgat aggttctact
    11521 gatatgggta caggaagtga tacgatttta gctcaaatgg ctgctgaggt tcttgataca
    11581 agcattgaaa atataacagt tatatcagct gatactgact tagttccata tgacccaggt
    11641 tcatatgctt caagtactac ttatgtaaca ggtatggcag tagttaaggc agctcaagac
    11701 ttaagagaaa aaataatttc tgctggagca actaaactag gtgttgagaa ggatgaagta
    11761 gaatttgatg gtataaaatt aactagtaaa aaaggtagca ctttattatt cgatatagca
    11821 gttgattcta caattggtcc agatactaat caattaatag gtcttgcttc tcatggtagc
    11881 ccagtatctc ctcctccttt tatagcaggt tttgcagaaa ctgaaattga cacagaaact
    11941 ggaaaagttg atttagtaaa ttatgtagct gtagtagatt gtggaacggt tataaataaa
    12001 aatctagcta agatacaagt tgaaggtgga attgttcaag ggattggaat ggcattattt
    12061 gaagaagtta gatacacaga aaaaggaagc atggacacaa atacatttat gcaatacaag
    12121 attccagcta gatgtgatat tggaaatgtt aaagtagatt ttgttgaaac ttatgaacca
    12181 actggaccat ttggtgctaa atctgttgga gaagttgtta taaatacacc tttaccagca
    12241 atacaagaag ctgtgtttaa tggaattggt gtaagaataa atacactccc tattacacct
    12301 gaaaaagttt ttatggaaat gaataaataa ttatttatga aaatttagac tagttttaca
    12361 tgttaaacaa ccagttatgt catacctgtt gaaagatatt taataagtag ttattataat
    12421 attatgaatt ttacttataa aatacaaaat taaataaggg gatgatgttt tggctggtaa
    12481 tctaaataat atgagagcag taaataattt tagaggagat aagaacattt tagaatgttt
    12541 agtcagcttt gagggtcgtt caataagtca gagaaaagta agggtatttt ttaaagaaaa
    12601 acaaaatcaa atagaaattg attttgcaga agaggaaatt tctaaattgg ttgaaaatgt
    12661 tgttttaaat acatcatatc aagaaatgtt atatgatgaa atagagaaac aactggaaat
    12721 tgattgtata ggtacttgga tgatattatc taaattaaaa gatggtagta gagttcactg
    12781 attttaaaat aaattattag agcctaattt taaggctctt ttttgctgtt tttaattaac
    12841 aatagaaaat ttatctttag tttgtatata att
//
LOCUS       DAENCV010000104        12758 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAENCV010000104 DAENCV010000000
VERSION     DAENCV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.105"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(228..1478)
                     /locus_tag="KQD82_002912"
     CDS             complement(228..1478)
                     /locus_tag="KQD82_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG4381370.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRMIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1510..2451)
                     /locus_tag="KQD82_002913"
     CDS             complement(1510..2451)
                     /locus_tag="KQD82_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG4381371.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2691..3767)
                     /locus_tag="KQD82_002914"
     CDS             complement(2691..3767)
                     /locus_tag="KQD82_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG4381372.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4140..4655)
                     /locus_tag="KQD82_002915"
     CDS             complement(4140..4655)
                     /locus_tag="KQD82_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG4381373.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5018..6088)
                     /gene="leuB"
                     /locus_tag="KQD82_002916"
     CDS             complement(5018..6088)
                     /gene="leuB"
                     /locus_tag="KQD82_002916"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG4381374.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEELAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6095..6586)
                     /gene="leuD"
                     /locus_tag="KQD82_002917"
     CDS             complement(6095..6586)
                     /gene="leuD"
                     /locus_tag="KQD82_002917"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG4381375.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6601..7878)
                     /gene="leuC"
                     /locus_tag="KQD82_002918"
     CDS             complement(6601..7878)
                     /gene="leuC"
                     /locus_tag="KQD82_002918"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG4381376.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7835..9577)
                     /gene="leuA"
                     /locus_tag="KQD82_002919"
     CDS             complement(7835..9577)
                     /gene="leuA"
                     /locus_tag="KQD82_002919"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG4381377.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9701..9947)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10095..10541)
                     /locus_tag="KQD82_002920"
     CDS             complement(10095..10541)
                     /locus_tag="KQD82_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381378.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10562..11281)
                     /locus_tag="KQD82_002921"
     CDS             complement(10562..11281)
                     /locus_tag="KQD82_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG4381379.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11399..12673)
                     /locus_tag="KQD82_002922"
     CDS             complement(11399..12673)
                     /locus_tag="KQD82_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG4381380.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3857 a         2449 c         1310 g         5142 t
ORIGIN      
        1 taaatacaca tttaaaacct tataaatata caaatacaat tatatgaatt ttcatcctca
       61 attatagcat agaatatgac tatctatatc ctaaaattat ccatttctaa aaaaaaggta
      121 actattatta agaatatcaa cttatagaat ttatattcca catacgattt tcaatcaaaa
      181 aaacaagggc taaaaagtga attgtgcact ttttagccct ttattaatta ttcatttatt
      241 tcatgtaaga atagaccaca tcttacttta ggttcaaacc atgttgactt agctggcatt
      301 atcttatttg catctgctac tcttattaaa tcttcaatac tagtaggata cattgcaaat
      361 gctactttca tatcttcatt aactcttttt tctaattctt caagacctct aattccaccg
      421 ataaaatcaa ttcttttatc tactctagga ttatcaattc ctaaaatagc atcaataaca
      481 tagtcttgta atatagaaat gtctaatgaa tcaacagggt cttctatttc taataaattt
      541 ttatttgctt tcattttata ccacttatca cctaggtaca ttccgaaagt tccttttttg
      601 tcaggtttat atttttcttt tccttctatt tcttctagtt caaaattttc ttttatttta
      661 tttataaatt cttcctcgct taaaccattt aagtctttga taaccctatt ataatccaaa
      721 accattaaat tctcatctgg tgcaatcatt gctatgaagt aattaaattc ttcttttcca
      781 gtataatttg gatttttagc tctcatttct ttggctacat tttctgctga agctgtccta
      841 tgatgaccat ctgcaatgta tagataatct aatttttcaa atttatctac taggttatct
      901 attatatcat cattatcaat cttccatact gtatgctcta tcttgtcatc tgttatgaaa
      961 tcatataaag gttcattatt atccataaaa tcattgatta tatcatctat catcctttga
     1021 tgtttgtaag ttacaagtat agttcccgta ttagcatcac agtattttat gtgtttagtt
     1081 ctatcaattt ccttttctgg tcgagtgtac tcatgttttt ttattgttcc attcaagctt
     1141 tcatcaactg atatacatgc tactatacca acttgagctc taccatccat gatttgttta
     1201 tatatatata tacactcttt gtcatctttt actagaattt cttgttttcg aaactcatct
     1261 aaattttctc gcgcttttaa ataaactttt tcatcatata aatctatatt ttcatctaaa
     1321 tcaatttctg atttatctat atgcaaaaat gaatatggat tcccttctgc aattcttcta
     1381 gcttctttac tattcataac atcgtaagga agtgctgcta ctttatctac tatatattta
     1441 tttggtctaa tcgccttaaa tggtttaatc gttgccatta ctaatttcct cctaattata
     1501 caaaatattt tataatgcta cccctattag attgttgaag tcaagaaaga aatcttgaat
     1561 cacatttact atttcgtctc ctattctttc ttgagcttct actgtagatg ccccaatatg
     1621 aggtgtagga gaaactcttg gatgatttac taagtctaaa tttaatgtag gttcttgttc
     1681 ataaacatct atagcagctg ctgataattt accttcatct aatgctttta gtaaatcttt
     1741 ttcatcaact aaaccgcctc ttgcacagtt tattaaataa gcccctttct tcatcttatt
     1801 tatttctttt tcagttatta ctgccccctt attcttatca aatggtatgt gtaaagataa
     1861 gaaatctgca ttttctaaaa cttcttccat atcacagaat ttataattgt taaagccttg
     1921 cgcctcaccc attatgtcag tatatataac attcatacct aataattctg ctctctttgc
     1981 aacttctttt gcaattcttc caaaacctat aagtccaagt gtctttccat taatttctgt
     2041 acctttatac tttttcttct cccattttcc atctctcata gttacatttg cagtatttat
     2101 gaatcttgca agaacaaata attgacctat tgtaagttct gcaacagata tactgcttgc
     2161 atttggagta ttcataactt ttatgccatt agcttgagca tatttaacat ctatattatc
     2221 cagacctact ccacctctta ctatcaactt caacttttta ccctctaatg ctttatctat
     2281 tacatcttta gtaactttag tagctgaacg tactacaagt acatcaacat ctttaagttt
     2341 atctcctaaa acatcttttt cataaaactg ctctatcaca ttaaaatcta actctctcaa
     2401 ttttctcgct gcttctttct ctattccatc agtaactaat atattataca taacaaactc
     2461 ctccaatttt tattcactta atttttccaa tttttatttt ttaaagtatt ttcatcaata
     2521 aaaaacttta cttaattcaa ctttctgttt atataatttt caaaatattt ttttattaaa
     2581 gtcatatcaa atttttctat tgtttgttaa aaatttaata tgtaattttt ataaaatatt
     2641 atttattata tattctaaat ttatcttaaa acatacatat ttttacaata ttatttatta
     2701 aaacctaata tatcatcaat attatctaat aattcttgta catcttcgac agtatagtca
     2761 cccatatgtg aaattctaaa agttttttct tttaaatctc catagccatt tgctatttgg
     2821 aatcctcgtt cttgtaattt tgaatttaaa tttgatacac taataccttg agtattttct
     2881 ataacagtaa gtgtattgga taagtgattt tcattggtaa aaatttggaa gtgtttttta
     2941 gcccagtttc taactaagtt tgccatgtct tcatgtctgt tgaatctgtt atctaggcct
     3001 tcatctaata tattatctaa ttggaaatct aaagcgaaca tgtgagaaag agatggtgtt
     3061 gaagggtatt ggtagttttt cttgattaag tattcataca ttgctaacaa atcaagatat
     3121 actcctctaa aaggtacttg cttagctcta tcgattgctt tttgagaaaa tgtgcaaata
     3181 gacattcctg gtggtaatcc cagtgctttt tgtactgatg ttatacaaat atctattcca
     3241 atcttatcaa cctcgacttt tattccaccg gctgaactta cagtatctac acagtagatt
     3301 acatcttcat actttttaac aacttctcca atttcttcta ttggatttct tattccagtt
     3361 gaagtttcat tgtgagttac agttattaaa tcgtattctc cggtagaaag caccttatca
     3421 accatttctg gcgttgttgc ctctcctaat tcaactttaa atatatccgc tgggacatta
     3481 ttagccactg ccattttgta ccatctatca ccaaaggatc cacaggaaaa tactgctgcc
     3541 tttttggatg tacaggaacg aatggagcct tccattaaac cagttccgga tgacgtggat
     3601 aaaagtatcg tgttgtttgt atagaatagt ttttgcatct tttcacttat actcttttgg
     3661 agcatagatg catcttttcc tctatgtcct atcattggtg tagccatctt ttgcaagact
     3721 tcttctttaa catcaatagg accaggaata aataatttct tactcattat aattcccccc
     3781 tttttagttt gtaatatgga tagcgttttt actgtcctta tttttaataa ctataatatc
     3841 aagctgaatt aactatgtca acataaaatt tattttttat atataattta ttttatatat
     3901 tttatgaaaa cattcatttt ctaccttttc ataagtctga attttctgaa ttattctctc
     3961 aaaaaaaata tactttttat ctatataaat taattttttt agtatgtaaa tttgacgcta
     4021 ttacgataaa tctttttagt ataactaatt tatttttatc ttgtattttt attgtatttt
     4081 taaattttat tttttctaaa tataaaattt atgttttaaa atgtaaaatt tttatttaat
     4141 caaagttcaa tatattccac tttttctttc gattcttcaa aaaaccattc ttcattagaa
     4201 aaatcacctt ctgcaatttg tccaataggc attttcatat actttccagt tgctgtcact
     4261 gctatatctc catttggtag aattatttct cctgtacctt caaataattt tctgttttct
     4321 ttagttattc ttccaacaac ttttatagta gcatctgttg gtactggttt cttatatttc
     4381 aactctaaag atactgttac tccccatatt tggtcatcat ttatacttac agctcttcca
     4441 atagtctcat ccaatattgc agcagacata cctccatgaa ctctcccagg ataactttga
     4501 tgccaatcct ttgtattaca aattgataca agctctccat tttcaagttc ataaaaatct
     4561 gctttaagtc ctagctcatt ttgtgttcca caaactaaac aagatttact actattttgc
     4621 ttcttcaaaa ctttatactt tgccaaaaaa tccatctcct aaccgacatt agtagtcgct
     4681 taatattaaa taattctaaa atatattata taatattaat aaaatccctg caaacttcta
     4741 ataataaaaa taggctactt caatatgata agtttaattt aaagtattat tttccaaaaa
     4801 ataactttat caaaatagaa actcaaagta gatataagcc tctttttcgt tattaaaata
     4861 gtaaaaatat attttactta atataaaaaa gagtgtctca ttaactaata aagtttattt
     4921 tgagacaccc ctcataaaac aaattatact acaatagatt tttactaatt ctacaagtta
     4981 tatattcaaa ttatattatt caaattaaac tataattcta aatttcattt actattaatt
     5041 cgcccattgc tcttgttcct acaacatttc ccacaccatt atagatatca atagttctat
     5101 aacctttatt gagtacactt tgtacagcat cttcaataca cttagcttct tcttctaagt
     5161 taaaactatg tcttaacatc atgcttacag atagaatagt cgcaattgga tttacaatat
     5221 cttttccagc tatatcaggt gcacttccat gtattggttc atacatacca aaactatctt
     5281 ctctaagtga tgctgatggt aacataccta tagaacccgt aatcatagat gcttcatcag
     5341 atattatatc tccaaatata ttattagtca ctattacatc aaattggcta gggtctttta
     5401 caacttgcat agcagcatta tcaacataca tatgtgacag ttctacatct tcaaactctt
     5461 tagctaattc ttccattata cttctccaaa gtcttgaact ctcaagcaca tttgccttat
     5521 ctacacttat aagtttttta tttctatttc tggctatttc aaaagctctc ttaccaattc
     5581 ttcttatttc attttcgtta tatatttcta catcataagc attttcgata ccatcaatga
     5641 tttttcttcc acgttctcca aaataaatgc cccctgttaa ttcccttaca acaacaaaat
     5701 caatgccttt ctcaactata tccgttctaa gtggtgaagc ttcttttata ctttcataca
     5761 ttgtagctgg tcttaaattt gcaaataaac ccaatgactc tcttaatttt aaaagaccag
     5821 actctggtct tttatctccc ttacatttat cccactttgg tcctccaaca gcaccaagca
     5881 atacagcttc gttttttcga caagtttcaa cagttttatt aggtagaggt tctccagttt
     5941 catcaatcgc gcatcctcca gctaatacat attcacaatc gaatctatga ttaaatttac
     6001 aacaaatttt atttagaacc tttatacctt catctataat ttccggtcca acaccatcac
     6061 cttttatgac tgctatatta caattcatac tccactaccc cctatttgct tttattgagt
     6121 taattaagcc ttcatttaat ataatatttt gcataaactc aggaaatggt tctgctttat
     6181 attcttttcc ttttgttata ttttttatca cacctgtaga aaaatctact tctatattat
     6241 ccccttcatc tatgttattg gcagcttctt cacattcaag tataggaagc cctatattta
     6301 tagagtttct aaaaaatatt ctagcaaaag tagaagcaat cacacaggat actccacttt
     6361 cttttattac aattggtgca tgttctcttg atgaaccaca accaaagttt ttagttgcaa
     6421 ctataatatc tccttttttt accttagaga taaatttatc atcaatatcc tccatacaat
     6481 gcgttgctag ttctttataa tcagctatat ttaaatatct agctggtatt attacatctg
     6541 tatctatatt gtcaccgaat ttaaaaacac ttccattggc aatcataaat tttacctccc
     6601 ttaaattatt tctgatggtt ttgatatttt tccagttata gctgatgctg ctgctacagc
     6661 tgggcttgca agatacacct ctgaatcaac atgccccatt cttcctacga aattcctatt
     6721 tgttgttgaa atggattttt ctcctgctgc aagtataccc atatgtcccc ctagacatgg
     6781 accacaagtt ggtgtagaaa ctgctgctcc tgcctctata aaagtggtaa tatacccttt
     6841 ttctatagct tcaagatata ctttttgagt tccaggaaat atcataactc taactccatc
     6901 tgctactttt ttgcctttca ttatctcagc tactacttca aggtctgata ttcttccatt
     6961 tgtacaagag cctattacaa cttggtctat ctttatatcc ttatctatct catctactgt
     7021 tctagtattt tcaggaagat gaggaaaggc tactgtttct tttagtttgg ctaaatctat
     7081 ttcgtatact tcatcataga ctgcatcttc atctgcttca tatatattta catcttttgt
     7141 catagttgaa cacttatgtg aatttatata ttccatagtc ttatcgtcta ctggaaaaat
     7201 tccatttttt gctcctgctt ctattgccat attacaaata gtaaatctat catccataga
     7261 taagtattct actccatctc cacaaaattc cattgacttg taaagtgctc catcaacacc
     7321 tatctcccct attatatgaa gtattatatc tttaccactt atccatttat ttggtttatt
     7381 tttcaaaaca aattttattg ctccaggaac cttaaaccaa cattttcctg tagccatacc
     7441 tgctcccata tcagttgaac ctacaccagt agaaaatgct cctaatgctc catatgtgca
     7501 agtatgagag tcagcaccta taattacatc tccagcagtt actatacctt tttcaggaag
     7561 aagaacatgc tctattccca tatctcctac atcaaaaaaa tttacaactc cttttgattt
     7621 tgcgaaactt cttgtaaatt tacattgttc tgcactcttt atatctttat taggtgtaaa
     7681 gtggtctaaa accatcacta ctttagtctt atcataaaca tcttcaattc ctaatttttc
     7741 aaattctcta atggctactg gtgatgttat atcattacct aaaactacat ctaaatctgc
     7801 ttcgattaat tgccctttct taacttcatt taatttagca tgtttggcta atattttctg
     7861 cgtcatagtc atccccattt tttatttcct cctcaatata acttttattt attgcattca
     7921 taagtgcatt aatagctgct gtatttatgt tttccgatat acttacacca aaatagactt
     7981 cattattgtg ctctattcct acataagcta cccctcttga atgtgaccct tcctctaatg
     8041 catgttccca gtaatatcta aatttataat ctttcatgcc acattgcttt agtgcattat
     8101 taaatgaatc tacaggtcca tttcctatac caacaatcct ttgttcattt cccatataac
     8161 ttattcttgc agtcatctca atcatgttag tatcattaga atcatcattt tcatagttta
     8221 attcatccat tgattttatt ttatacttct taactttata agggctctca acttcaatat
     8281 attctttctt aaacaaatta aatattgctt cactactaag ttcttcccca attctatcag
     8341 attccatttt gactacattt ccaaaatcag aatgcatatt cttaggtatc ataaatccat
     8401 aatctgtttc cattatataa acagctcctc cttttccaga ttggctattt atccttataa
     8461 tttctttgta ttctcttcca atatcatgag ggtctattgg taaatatgga acttcccagt
     8521 aatcattatc tctgtttttc attgctatca tgccttttct aatagcatct tgatgtgaac
     8581 ctgaaaaagc acaatgtaca agattacctg catatggatg tctatcatgt accataagtt
     8641 ttgtacagtc ttcatatata cctattatct tatctatatt gctaaaatct agctctgggt
     8701 ctattccttg tgtataaaga ttaagtccca catttactat atccatattc ccagttcttt
     8761 caccatttcc aaatagtgtt ccttcaagtc tatcggcacc agccaataat ccaagttcac
     8821 ttgcagccgt acaagttcct ctatcattgt gagtatgcaa acttagtata atagaatccc
     8881 tacaacttat atttttacaa aaccattcta tttggtctgc atatacgttt ggtgttgcca
     8941 tttcaactgt tgatggaaga tttattatta cttttttttg aggagttggt ttccaaacat
     9001 ctatcactgc ctcacatatt tcaagtgcat aatccatctc tgttcctgta aaactctctg
     9061 gtgaatattc aaaagtaaat tctgtttctg gatatttttt tacttcttca ttaaataatt
     9121 ttgctccttc aactgctata tctattattt cttgtttact catattaaaa accacatctc
     9181 tttgaagaac agatgtagag ttatataaat gtataattgc ttttttacat ccccttattg
     9241 attcaaatgt tttttctata agatgagccc tagattgtgt aagtacttgt atagttacat
     9301 catctggaat catattctca tctattaatt ttcttaaaaa tgtatactct gtatttgatg
     9361 ctgatggaaa tcctacctct atttctttaa atcctgtatc aataagcatt ttgaacattc
     9421 tgactttttc atttacactc ataggtgttg gcagtgactg atttccgtct cttaaatcga
     9481 cactacacca aattggagcc ttaaatattt cattatctgg ccatttacga tttactattt
     9541 gcattttatc atatttttta tactttccac acttcatgtt atttccccct ttttctgttt
     9601 tttgtattca gtatacctca tatcgacgca atcctgcaca attcataagg ataaatgtca
     9661 attttatcat tagaataaaa aaaacctcat ctctaataaa attagagacg aggttttgct
     9721 cgcggtacca ctctatttag ctttctaaaa agctccctta gtaatagcca atacaataat
     9781 tataatgact atatttccta taacgtggaa ttactccgat tattctaaga tttaaatctt
     9841 agaaaacagc tcctagacaa gttcagaact atcaactact aaatcacacc atcctttagc
     9901 tctctgaaag tttcattgtt cttactactt ctattcatca cttttttaat catttaaatt
     9961 ttctaaatat tttgatttaa tagttattat atacacatta aaatgtattg tcaacacaaa
    10021 aaataattat ctcaattttt tatttttgat aagcaattct ataaaacatg ctaactattg
    10081 aaaacagtat gatattatgc cttaaacttt ttcatctttt catctatgat attcaataat
    10141 tttttttcat cttccaaact caatgccctt aatctaattt tataattaga ttcgtcagtg
    10201 tatatattta atatattttt tactacttct attttttcta ttatcttata attaaacttt
    10261 tgactttgtt tagattttat actgaatata cattgattat ttattaatat tgaatgatta
    10321 tcgagagtaa tatatacttt tccctcatat ttatttgaaa aatcacgacc ttgtttaaat
    10381 agaaaaactg ctgttattat acataaaata tataatattg tcgttgaata tataaaaatt
    10441 gtagctaaaa ttataagtgc taaaaccata tatatagtca ttcctttttt aaaataacca
    10501 ttgtcataat aattcatata gataaactta tttttactca aattttttca ctcccaagat
    10561 tctaaaaatt tatatcaaaa aattctataa tttcatcttc atgataattt atgactaatt
    10621 catctggaaa atctactgct tctaaaactt tttctgcatt ttcaaattct cctatagaat
    10681 aacatatatg agagtcagta ccaagaatta ctcttgcacc atatgtttta cacaatgtca
    10741 acatatgact tacattttcc caagtacccg ttctatgaga atttgaactt aatgatgaat
    10801 tatttatttc taaaaggata tttttatctt tagctttttt tactataggt tcataatcta
    10861 aaggatacct actgtcatct ggatgtccaa ttatttttac cttaggttta tccataactt
    10921 ttaaaatagc atttgtattt tcttcttttg taccagaagc tatacaaggt ctatgcaaac
    10981 ttgcgattat ataatccaaa tgttgcaaca tatcttcttg aacatctaaa ttaccattat
    11041 aatccattat atttccttct attcctctaa gaatctttac gccttgtact tcacgtggaa
    11101 gcaaatgcaa attataaaaa tagaatggat gtggtcctcc tggcatatta ggtccatggt
    11161 ctgatattcc taagtatttt atattcttct tactcgcctc ttcgacattt tccttgaaag
    11221 tactatgtgc atgtccactt attagagtgt gacaatgtac atccataata gactttttca
    11281 ttaaatttcc tcctaatatt ttacataaat tcgtgaaaat agctaaaggg ctatttcaaa
    11341 actaaagctg tcataataga cagtgtaacg ttttaaaaca gccccatttt caattatttt
    11401 acacttctaa atctttaaat ttatctttaa ataattctcc aatacttcct aaagattcct
    11461 ctacttctag gtattctgtt atatcctctt ctggctctct agttgcttct tttattgaaa
    11521 ccaacattct tttagtcttt ggtttaaaat caagaatctt aactttaacc tcatctccaa
    11581 tattaacaac attagtaact ttagccactc tagtatcact caattcagat attggtaaat
    11641 acgcatctac accttcttta atttgaacaa aagccccttt ttctattatt ctaagaactt
    11701 tagcattgac aacatcatca atttgtactt cttttgctat taaatcccaa gggtcattat
    11761 ttatgtcttt taatgccaag gcaatttttt tagtctcttt atttacatcc aacacataaa
    11821 cttctacttc ttgtccctct gaaacaacat cttcaacact ttcaactctt gtccaagcta
    11881 aattatttat atgtgctaat ccttcaatcc caccaatatc cacaaatgca ccataaggca
    11941 ttatctttgt caccttacca tctctttttt gtccaacttc aagagaatta aataagtctt
    12001 ctttagctgc tttgatacga gccttttctt cttctctttc ttgcttaatt cttgctcttt
    12061 cagcttcttt ttctgcctta atttgagctc ttctttcttc tctctcttta tttatacgct
    12121 cttgtaatac atctctatga gatgctacaa gtctattttt tctaacatcc aattctttta
    12181 tataaacttc taagttaaga ccaacaaatt tagatgtatc tgtaataaat tttgtatcaa
    12241 gttgagaaat tggtataaaa aatgtatatg tattgtaatt agcaaataca cctttatcta
    12301 tattcttttc aacatttaca gttattattc tatgttcatc ataagccttt tgtaactcgg
    12361 caaaatcatt tcttttgtca agttgtaatt tagataaagt tattgtacca tctttttgag
    12421 atacttttgt tattacagca gttatttcat cacctatgtg gtatatatct tctatatatt
    12481 gatttttttc tatgttcaat tcacttatag gtattattcc atcaaaccca tagtctaatc
    12541 ctaattgtac ttcatcattt ctcactgcag ttatttttcc agttgtcaat tctccaactt
    12601 tcactttgct gaatacttgt tcttgctggt caagtaattc ttgcattgtt aaatcatttt
    12661 ccattataaa catcctttcc tcttattgat tatcatatct gtaaatatta taccattaat
    12721 attgcaaata cttacatttt tcttactata atttttaa
//
LOCUS       DAENCV010000105        12723 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAENCV010000105 DAENCV010000000
VERSION     DAENCV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.106"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            61..1755
                     /locus_tag="KQD82_002923"
     CDS             61..1755
                     /locus_tag="KQD82_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG4381381.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKIYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2087..2197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2301..2672
                     /locus_tag="KQD82_002924"
     CDS             2301..2672
                     /locus_tag="KQD82_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG4381382.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2767..3714
                     /gene="trxB"
                     /locus_tag="KQD82_002925"
     CDS             2767..3714
                     /gene="trxB"
                     /locus_tag="KQD82_002925"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG4381383.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3799..4116
                     /locus_tag="KQD82_002926"
     CDS             3799..4116
                     /locus_tag="KQD82_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG4381384.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4385..5671
                     /locus_tag="KQD82_002927"
     CDS             4385..5671
                     /locus_tag="KQD82_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG4381385.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5783..6256
                     /locus_tag="KQD82_002928"
     CDS             5783..6256
                     /locus_tag="KQD82_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5915..5917,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG4381386.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6288..7598
                     /gene="grdB"
                     /locus_tag="KQD82_002929"
     CDS             6288..7598
                     /gene="grdB"
                     /locus_tag="KQD82_002929"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7335..7337,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG4381387.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7855..9387
                     /locus_tag="KQD82_002930"
     CDS             7855..9387
                     /locus_tag="KQD82_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG4381388.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9398..10543
                     /locus_tag="KQD82_002931"
     CDS             9398..10543
                     /locus_tag="KQD82_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG4381389.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10853..11932
                     /locus_tag="KQD82_002932"
     CDS             10853..11932
                     /locus_tag="KQD82_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG4381390.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHAAIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12045..12644
                     /locus_tag="KQD82_002933"
     CDS             12045..12644
                     /locus_tag="KQD82_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381391.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
BASE COUNT         5049 a         1362 c         2489 g         3823 t
ORIGIN      
        1 agtacatgta aatctatatt tataaataca ataaagtaaa gttgtaaagg agtgctttaa
       61 atgaagtttt cagagtttaa gtatgaaaga cccaattata atagtatgaa aaaagagttt
      121 ttgagttgtg tagaagatat aaataattct cgtaattata aagagcaaca aaaaaatata
      181 cataagatta atttattaag aaataaaata gaaacgttgt caaatatagc ttcgataaga
      241 tatagtacag acacatttaa taaattctac aaagaagaaa aaaattattg ggatgaatat
      301 atgcctttgt atgaagaatt aaattcatat ttttataatg ccattgtaaa ttcaaaattt
      361 aaatatgatt tgataaaaga gtttggagag caatttttca caatagtaga gtattcttta
      421 aaaagttttt ccaaagaaat aataagtgaa ttgcaggaag aaaataaatt gtgttcagag
      481 tatactagac ttttagcttc agctgagata atgtttgatg ggaaaataag aaatttatct
      541 ggtatgggga aattcatgta ttctaagaat agaaaaacaa gagaattagc aaacaaagca
      601 tattataatt tctttgaaga aaatgaaact aaatttgatg acatatttga taaattggtt
      661 aaacttagag ataagatgtc taaaaaatta ggttttgaag attttgtaga gcttgggtat
      721 gttagaatga tgagaagtga ctacagagaa tatatgataa aaaatgttag aaaacaagta
      781 ctaaaatatg ttgttccaat ggcaaatgag ctttatgaaa aacaagctaa aagaattggt
      841 ctagaatatt taagttacat agatgaaggc gtggaatttt taacaggaaa tgcaagttta
      901 aagggagatt caagatacat aattcaaaat ggtaaaagga tgtattctga actgtcaaaa
      961 gaaacaaatg aattttttga ttttatgtta gaaaatgaac ttatggattt agaaacgaag
     1021 aaaggaaaag gagctggagg gtattgtact tatataccag attataaatc accattcatt
     1081 ttttctaatt ttaatcaaac tgctgatgat atagatgtgt taacacatga ggcaggacat
     1141 gcatttcagt tgtatatgtc tagatggatt gatatgccag aaattaattt tccaacgctt
     1201 gatagttgtg aaatccattc aatgagtatg gaatttataa catggccgtg gatggattta
     1261 ttttttaaag aagatacaga taagtacaaa ttcactcact tatcatcttc aattaaattt
     1321 ataccttatg gagtaattgt tgatgaattt caacactata tttacaaaaa tcctaatgta
     1381 gataagtcaa aacgaaaaga aatctggaga tttttagaga aaatatactt acctcataga
     1441 aaatatggtg ataattcttt tttagagaga ggttgttggt ggtttaaaca aggacatata
     1501 tttaaaaatc cgttttatta tatagattat gtattagctc aaatttgtgc gttacaattt
     1561 tggaaaaaaa tgattcaaga tagagatgca ggatggaaag attatataaa tatctgtaaa
     1621 gttggaggaa caaaatcatt tttagatatt gtgagtatgg gaaatttgta ttctccattt
     1681 gatgatggct gtattgagtc tattataggt gatgttaaat cttggtttga tgaaataaat
     1741 gacagcaaat tataaaaaat tatgagaatt ttattgtata aaagtgaaaa attaaaaaat
     1801 atataaaaag tgattatttg ttgtgaaaat agtcttgttt ttaactaaat tgataaaata
     1861 aactaaaaaa gatttgtact taaaaaaaaa agaaaataca agaaatatgc aatatcaaca
     1921 tttggaaaaa aaagtaaaaa tgtatacaaa atacatataa aaaaactatt gcaaatttta
     1981 tagaatgttt atatacttaa gtcaacagag atagaaaaaa gctatttata gaaaacaaag
     2041 gataaattgc ttaattttaa attttctgaa aaaaataata atccggatga agagtatggg
     2101 agactgcttt ataaaaagcg accgaaggga caagtttaat cttacttacc caaaagatta
     2161 gatgaaattc tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat gattttaaca
     2221 gggaagacaa attgggagat ttgtttttcc tgttttttat ttatcaagta ataaataaaa
     2281 gtttgaaaga gagtaaaaaa atgataataa ttacgaataa tccaaaagta aaagaagaag
     2341 ttcaaggcag agaagttttg tttaaagaca caacttatat tggaatctta gaagcaagta
     2401 gagatttgat acatgaaggt tatgaacttt tatctcatcc actctatggg agtgtaaagc
     2461 caaatgaaac accttacagg actgttatac taaagaaagg aaatcgtttg gatataaact
     2521 ctttaacctt aattgaagaa gcaatcatca ctgcaagtaa atttcagaat aataaaaaga
     2581 ctccaaaatg gacagaaagt gttcaagatg attttagagt aattgattat gatatatttt
     2641 ataatacaat acaaagaatg cagtatgaat aaaaaaattg ttaaaaagtt attaaaacag
     2701 cctaaatatt aaatctaatt taatattaaa taaaatgaga aatttaattg gggaggataa
     2761 gtcaaaatgg aaaatgtgta tgacttagta ataattggtt caggaccagc agggcttgca
     2821 gcaggtcttt atggagcaag agcaaagtta aaaactctaa tcctagaaaa agacaagact
     2881 ggtggacaaa ttgtaataac acatgaaatt gcaaattatc cagggtcagt tccaaatgca
     2941 acaggaccaa gtttaatagc tagaatggtt gaacagtgta aagagtttgg agcagaaatg
     3001 ctaagagaca acattgttga tactgagcta gatggagata taaaagtttt aaaaggggaa
     3061 aaggcagaat atagagcaaa agcagtaata ataggaacag gagcaacacc aagaaagatt
     3121 ggttgtcctg gagaaaaaga acttactggt aaaggggttt catattgtgc aacttgtgat
     3181 gcagatttct ttgaagattt tgaagtattt gttgtaggtg gaggagacag tgcattagaa
     3241 gaagcaatgt atttaactaa atttgctaga aaagtaacaa ttgtacatag aagacaagga
     3301 tttagatgtg ctaagagtgt tgaagaaaaa gcaaaagcta atccaaagat agagttttta
     3361 ttagatacag taattgaaga aatcaaaggt gatggaatat tagaatcagt tgtatttaaa
     3421 aataaagtta ctggagaaac tcatgaatac tttgcagatg aagaagatgg aacaatggga
     3481 gtatttgtgt tcgttggatt agatgctcaa actgatttat tcaaaggtaa agttgatatg
     3541 gatgaaaaag gttacataat aactgatgaa gatatgagaa ctaacatacc aggcgtattt
     3601 gcagcaggag attgtagatc taagactcta agacaggttg taactgcaac taatgatgga
     3661 gcaattgcat ctatagtagc tgagaaatac attgatgaaa aatttgggaa ctagaatctg
     3721 taaattaaga aatagtgtct gtaaatttaa tgaataatat ttaaaattaa aataaaaatt
     3781 tcaaggggga ataagaaaat gttagattta gataaggcta catttgaaga agaagtttta
     3841 aatgcagaag gttttgtatt tgttgatttt tggagtgaag gttgtgagcc ttgtaaagct
     3901 ttaatgccag atgttcataa attagctgag acttatgggg ataaaataaa attctgtaag
     3961 atggatacaa caaaagctag aagattagct ataaaacaaa aagtattagg tcttccaact
     4021 atggctatat acaaagatgg agaaaaagta gacgaagtta ctaaggatga tgctacagtt
     4081 cctaatatag aaaacatgat taaaaaatat ctttaatatg ttgataaatt attaacatta
     4141 aaagaaaaaa tctattttta tctgaagata agaagaaaaa ggtttttatg tccaactttg
     4201 ttaaaaaaat aattttaaat aaaataattt ttttacaata aatatttttt agtaataaaa
     4261 taaagattta aggcataaaa acacaataat atatctttaa aaaaataaga taaattgtaa
     4321 aatgatataa ttttgagaaa aactcaaatg catatacaca aattaaataa caggaggtgc
     4381 aagtatgcgt cttgaattag ggaaaatctt tataaaagat attcaatttg gtgatgtaac
     4441 agaagtaaaa gatggagtat tattcatcaa taagcaagag atgctacaag aaattggtgg
     4501 agatgagcat attaaatcta tagatataga attagctcgt ccaggtgaaa gcgtaagaat
     4561 aacacctgta aaagatgtta ttgagccaag agtaaaagtt gaaggtaatg ggggaatctt
     4621 ccctggtatc atgtctaaag ttgacactgt aggagaagga aaaactcatg ctttaaaagg
     4681 tgtagctgtt gtaactactg gaaaaatagt aggtttccaa gaaggtataa tagacatgac
     4741 tggagaagga gctaagtaca ctccattttc taaattaaat aatgtagtag taatagctga
     4801 acctattgat ggtttaaaac aatacgctca tgaaaaagct gttagaatga taggattcaa
     4861 agcagcaatg tacttaggag aagtagcaag aaatcttact ccagatgaag tttcagtata
     4921 tgaaactaaa cctcttcttg aatcaataaa agaatatcca gagcttccaa aagtaggata
     4981 tgtttacatg cttcagactc aaggtttatt acatgatact tatgtatatg gagtagatgc
     5041 aaaacaaata gtaccaactt tattatatcc aacagaatta atggacggag caattgtaag
     5101 tggtaactgt gtttctgcat gtgacaaaaa cccaagttat gttcatataa acaatggggt
     5161 tgttgaagac ttatatgcaa gacatggtaa agatataaac tttgtaggtg taattataac
     5221 taatgaaaat gtttatctag cagataaaga aagatcttca aactggactt ctaaattatg
     5281 taaatattta ggattagatg cagttatagt ttcacaagaa ggttttggaa atcctgatac
     5341 tgaccttata atgaactgta agaagataga aatgcaaggt gttaaaacag ttatagttac
     5401 agatgaatat gctggacgtg atggaggttc tcaatcactt gcagatgcag atgtaagagc
     5461 tgatgctgtt gtaacaggtg gaaatgccaa ccaagttgta gttcttccta agttagataa
     5521 agttattgga catttagaag tagtagatgt aatagctggt ggttctgatg gaagtttaag
     5581 agctgatgga actattgaag ttgaaatcca agctataaca ggtgcaacta atgaaacagg
     5641 atttggtcat ttaactgcta aaggatatta agaagtattt tattaaacga ttgagtaatg
     5701 ttaataaaac atattataga taattataat aagtgttata gatatataaa aatatattag
     5761 aaatataata aggaggatta ttatgagttt acttagtaat aaaaaggttc ttataatagg
     5821 tgaccgtgat ggtataccag gacctgcgat agaagaatgt gtaaaaacag tagaaggagc
     5881 agaggttgtt ttctcatcta cagaatgctt tgtctgaaca gctgctgggg ctatggactt
     5941 agaaaatcaa aacagagtta aagatgctgc tgataaattc ggagctgaaa atgttgtgat
     6001 tttactaggt gctgctgaag ccgaagctgc aggtcttgca gccgaaacag taactgctgg
     6061 agatccaact ttcgctggac cacttgctgg agttgcctta ggattaagtg tttaccacgt
     6121 tgttgaggaa ccaataaaat cattatttga tgaaagtgta tatgaggacc aaataagtat
     6181 gatggaaatg gttttagaag ttgaagaaat agaagaagaa atgtctggta taagagaaga
     6241 attttgtaaa ttttaatgcc taaaagcaaa aggggtgaaa ctaagagatg ggaaaactaa
     6301 aagcagtaca ttatattaat cagttctttg ctggtatagg tggagaagaa aaagctgata
     6361 ctaagccaca tgtggcagaa actttacctc caataagttt acaacttgat aaattattag
     6421 gtgaagatat tgaaatagtt ggaacagtag tttgtggaga tagctatttc aatgaaaata
     6481 tagatagtgc aagtgaagaa gttttatcaa tggtaaaagg ttttgaacca caacttttca
     6541 tagctggtcc tgcatttaat gctggtagat atggggttgc tgctggaaca ataactaaag
     6601 ttgtaaaaga tgctttgaat ataccagctt taacaggtat gtatgttgaa aatccaggag
     6661 cagatatgtt caaaaaggat gtttatgtag tagaaacttc tgattctgca gcaggtatga
     6721 gaaaggcact tcctaagata gctaaacttg cagtaaaact tgctaatgga gaagaaattg
     6781 gaactcctaa agatgaagga tacatagcta gaggaataag agttaactac ttccatgaag
     6841 acagaggttc taagagagct gttgacatgt tagttaagaa aataaaaggt gaaccatttg
     6901 aaactgaata cccaatgcca aactttgata gagttgaccc aagtaaagct gtaaaagatt
     6961 tatcaaaatg taaaatagct ttagttactt ctggtggtat agttccaaaa ggaaatccag
     7021 atagaataga gtcttcttca gcttctaaat atggaactta ttctatagca ggagttatgg
     7081 atttaacaga agagacttat gaaactgcac atggtggata tgacccagtg tatgcaaatt
     7141 tagatgcaga tagagtatta cctgttgatg ttcttagaga tttagaaaaa gaaggtgtaa
     7201 ttggaaaatt acatgagact ttctatacaa ctgtaggtaa tggtacatct gttgcaaact
     7261 ctaaaaaata tgcttctgaa attggagcag cattagtggc tgatggtgta gatgcagtaa
     7321 tattaacttc tacatgaggt acttgtactc gttgcggtgc aacgatggta aaagaaatag
     7381 aaaaaactgg acttccagta gttcatatgt gtacagtagt acctatttca ttaacagtag
     7441 gggctaatag aatagttcca actatagcaa tacctcatcc acttggaaat ccagcgcttg
     7501 accctacaga agaaaaagcc ctaagaagag gccttgtaga aaaagcatta aatgctttaa
     7561 ctacagaagt agatggtcaa acagtatttg aaaaataaaa ataacacaca catatattat
     7621 atagataaaa atataaacac acactaaaaa taaaaaataa ctatacacaa aaaaaataat
     7681 atagatttca tgaatggata aattggagtt attcattcat gaaatcttaa aaaatgccaa
     7741 aatacataaa caataataga atcaaatata caataaaata caatgattag agcttttaat
     7801 aagtttaata agattaaaaa ataagtgttc ataaaattta tggaggtgca caaaatgact
     7861 tatccagttt taaaaggggc aggctatgta cttattcata ctccagatat gatagtgcaa
     7921 aatggaagta cttgtacagt tgaaagagca acaaatccag attcagaatt tttaaaagaa
     7981 gtaagtaatc atataagaag ttatgaagat gtagttaact acatgccaaa tcaagtttat
     8041 ataggaaata gaagaccaga agaattaaga gatttaccaa tgccatggtg tgaacaaaag
     8101 attgagggaa ctagaaatgg taagtttgga gaaataatgc ctcaagatga gttcatagct
     8161 ttaatgcaaa taagtgatgc atttgattta gtaaaattat cacaagaatt tattgatgaa
     8221 gtaaagccaa aaatcgaaaa taactaccct gaaattgcac catttgtagg taaattaaag
     8281 ggtgatgata tagaagaagg taaagaatta gtagcaacac acatagctga aggattatac
     8341 catgatggaa aatttgttgg ttatgtaaaa agagcacatg atgtagatgt taacttaaat
     8401 gcccatacaa tgtttgaaaa tctagtagta aaagcatctg gagttttatc tgctattcaa
     8461 atgctaagac atagcaaaat agaccctgct gaaatagatt atgttattga gtgttcagaa
     8521 gaagcttgtg gagatataaa ccaaagaggt ggaggaaact ttgctaaatc aatagctgag
     8581 atagcaggac ttcaaaatgc aactggttct gacacaagag gattttgtgc agcaccaact
     8641 catgcattaa ttcaagcagc agctttagta aaagctggta tacataaaaa tgtaatggta
     8701 gtagcaggtg gagcaagtgc taaacttgga atgaatgcta aagaccatgt taaaaaaggt
     8761 cttcctgtac ttgaagatgt tgttggagga tttgcagtac ttgtatctga aaatgatggt
     8821 gtaaatccag ttataagaac tgatttaaca ggtaagcata ctgttggaac tggttcttca
     8881 ccacaagcag ttatgacagc acttataact tcaggtcttg atagagctaa tttaaaaata
     8941 acagatgttg atgtgtattc agttgaaatg caaaacccag atataactaa gccagcagga
     9001 gcaggagatg ttccagaagc aaactacaaa atgatagggg cacttgctgt taaacgtgga
     9061 gacttagaaa agaaagaatt gaaagatttt gtttctaaca aaggattacc aggatgggca
     9121 ccaactcaag gacatatacc atcaggagct ccttatatag gatttttaat agatgattta
     9181 actacaggaa atagaaatag agctatgata gttggaaaag gtagtttatt cttaggaaga
     9241 atgactaact tatttgatgg agtatctttt atagctgaaa gaaatacagg tgttactgaa
     9301 gagacttcag gaatatctaa ggatgaaatt aagaagataa tagctgaatc tatgaaaaaa
     9361 ctagcattag atatgttaga agaatagggg tgaggatatg tcaaaaaaag taatcgcaga
     9421 tgtattctta gaagtagcta atgcaataga aagcggcgaa tttggcaaaa aagtaaaaat
     9481 tggtgttact acacttggaa gtgaacatgg tgtagaaaac atggttaatg gtgcacaatt
     9541 agcaaaatct aatttatttg atattgtgct tattggacca aaagtggaaa ctgaccttga
     9601 agtagttgaa gttaatgatg aaaaagaaat gcatgcaaaa atggaagaat tattagattc
     9661 aggatatatt gatgcttgtg ttactatgca ttataatttt ccaataggtg tatctacagt
     9721 aggtagagta ataactccag caaaaggaaa agaaatgata cttgcaacaa caacaggaac
     9781 aagtgctact aatagaatag aggcaatggt aagaaatgcc atttatggta ttgctacagc
     9841 taaatcaatg ggtaacaaat gcccaaaagt tggaatactt aatgttgatg gagcaagaca
     9901 agttgagaag tgcttaaaag aattaaaaga taatggatat gacatggagt ttgcggattc
     9961 aattagagca gatggtggat gtgtaatgag aggtaatgat ttacttgtag gtgcacctga
    10021 tgttatggtt actgatacat tatctggaaa tatatttatg aaagtatttt cttcatatac
    10081 tacaggtgga gattatgaag ctcaaggctt tggatacgga cctggagttg gagaagacta
    10141 tgatagaaag gtacttatag tatcaagagc atcaggttct ccggtagtag caaatgcttt
    10201 aaaatacgct tatgatgttg taaagggaga tataagtaat gttgcaagaa atgaatttgc
    10261 aaaggttaaa aaagcaaaat ttgatgacat tatatcctct cttactaaaa aagaagttaa
    10321 agctgaaaaa gtagaagtta aaatgccaga taaggaaatt gttactcgtc aaatagctgg
    10381 tgttgatata atggatttag aagatgcagt atctgagtta tggaaaaacg gaatctatgc
    10441 tgaaagtggt atgggttgta caggtccaat agttttagta aatgaagcta aaggagactt
    10501 agcagttgaa actttagtta aagctggata tactgcaaaa taagtacatt ttttatatgt
    10561 aaataagaac atatagttat aattttattg attataatta tatgttcttt ttattttata
    10621 tcttgtcaat atttatatat taacacaatc catttatttg attctctagc taatttatta
    10681 tatttattca attaaaatta agctttctaa ctaaataaaa actaatgaag tattaaaatg
    10741 aaattgataa aatatgaaaa aattttagga ataaagtatc tgttagataa aaataagatt
    10801 aattagtgat ataatataat taagataaaa tttattattt ggaggtaaaa tcatgagcag
    10861 agtaaaaaat gtagtagaat tgttggaaac taaaggtgtt gatgcactat atctaacaaa
    10921 gaaaactaat gtaaactata taagtggttt tcctgatgaa gaagcatatg cagtgatttg
    10981 caaagatgga aactttttag ttactgatag tagatatatg gagcttgcag aaaaggtttg
    11041 caaagatttt gaaattataa attggcataa ttttgataga agtgtggcaa aggcagttaa
    11101 aagtgtatgt gataaagtgg gtataaagaa acttgggttt gaaagaacta atatagtgtt
    11161 tgataaatat gaagaactta aaaatctgat tgagaaggat aatggagaat taatacctac
    11221 agaaaatatt gtagagactt tgagatatgt aaaagacaaa gatgaaatca aaaatacaag
    11281 aaaagcttgt gaaatagcgg ataaggcatt agaagaattg attccacata taaaagctgg
    11341 agttagcgaa attgaactgg ctacaaagtt agagtacttt atgaaaatga acggagcaca
    11401 aaatataggg tttgaaacta tactgatatc aggagcaaag acttcattgc ttcatggtaa
    11461 gcctagtgat aagattatag aaaaaggtga ttttgtactt atagactatg gtgctatgta
    11521 taatggctat atatctgata caactagaac ttttatagtt ggaggagctt ctgaaaagca
    11581 attagaaata tataatttag ttaaggaagc tcaaaatgta ggggttgaga acatgaaagc
    11641 tggtgtacat gcggcaattc cagatgctga gataagaaaa gttgtcaaaa aatatgaaga
    11701 ctattactat caaggtatag gacatggagt cggtagagat gttcatgaag aacctttcat
    11761 tggtaattat ggtgataaaa tcatagaaga gggatgtata ataacaatgg aaccaggtat
    11821 ttatttccct ggatggggtg gagttagaat agaagatact gttttgatta ctaaaaatgg
    11881 tccagaaaga cttactaaat tccctaaaga tttgatgatt ttagacaaat aatttaattt
    11941 atatatattt ttttacataa aatataacat aatctcacta ttaagctcta tttacatatt
    12001 atagtaaaga gggcttaatt ttattgtgag aggtgatagt atatatgtat aaaaaacata
    12061 aatttataaa aaatataaaa aaagcctggt ataaaattaa attaattgat agatgcctta
    12121 taattgtaat ggcaatatta atgtttcaat ctatatataa tttatttgtt aatgaagtaa
    12181 attcacaaga tactacaact attgatgtag tagtaagaac cacttcagca gctatatttg
    12241 ggtatttttt aagtgctaat tttataaaga gacctttaag gaagtataca aatactgatg
    12301 tatcttcaaa tctttttata gataataatt tatccccaaa aggtcaagag tctaatcaaa
    12361 ataatataat gaatgtcaaa aataccattg gatttacttc agaagctgat aattatgaaa
    12421 agaaaatacc tattaataat aatgaaggtt ttgaagaagg agaaactagt gaattgcaaa
    12481 ttattatagc tactgtaatt tgtatagtag cattaacagt actttttgtt gtaagaaatt
    12541 ttacaactac aacatcagct tctttaggta ctatttcaca gatgagagat tttgtctcag
    12601 gatgcgtggg ttttttgcta ggttgtccaa gtaaacctga ttaaaaaagt attatcaata
    12661 aaaggtattt taaaaagtat cagtataata atatttagat ttaaatgtat acaattataa
    12721 ttc
//
LOCUS       DAENCV010000106        12645 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAENCV010000106 DAENCV010000000
VERSION     DAENCV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.107"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(151..1038)
                     /locus_tag="KQD82_002934"
     CDS             complement(151..1038)
                     /locus_tag="KQD82_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4381392.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(1138..1587)
                     /locus_tag="KQD82_002935"
     CDS             complement(1138..1587)
                     /locus_tag="KQD82_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381393.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(1577..3484)
                     /locus_tag="KQD82_002936"
     CDS             complement(1577..3484)
                     /locus_tag="KQD82_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4381394.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQKEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(3753..5648)
                     /locus_tag="KQD82_002937"
     CDS             complement(3753..5648)
                     /locus_tag="KQD82_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381395.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(5684..6562)
                     /locus_tag="KQD82_002938"
     CDS             complement(5684..6562)
                     /locus_tag="KQD82_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG4381396.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILKAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(7105..7275)
                     /locus_tag="KQD82_002939"
     CDS             complement(7105..7275)
                     /locus_tag="KQD82_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381397.1"
                     /translation="MSALLYGVCNKPISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(7661..7873)
                     /locus_tag="KQD82_002940"
     CDS             complement(7661..7873)
                     /locus_tag="KQD82_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381398.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(7874..8290)
                     /locus_tag="KQD82_002941"
     CDS             complement(7874..8290)
                     /locus_tag="KQD82_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381399.1"
                     /translation="MSKSETGVRSKLILELIVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(8358..8771)
                     /locus_tag="KQD82_002942"
     CDS             complement(8358..8771)
                     /locus_tag="KQD82_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381400.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLISVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(9605..10234)
                     /locus_tag="KQD82_002943"
     CDS             complement(9605..10234)
                     /locus_tag="KQD82_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG4381401.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLRVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(10313..11275)
                     /locus_tag="KQD82_002944"
     CDS             complement(10313..11275)
                     /locus_tag="KQD82_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG4381402.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(12037..>12645)
                     /locus_tag="KQD82_002945"
     CDS             complement(12037..>12645)
                     /locus_tag="KQD82_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381403.1"
                     /translation="DSGNGGNSKPDSGSGGNSKPDPDNGGNSKPDPDNGGNGKPDPDN
                     GGNSKPDPDNGGNSKPDPDNGGNSKPDIDNGGNSKPDIDTDGDGKPDINIDTDGDGKP
                     DINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKPDVNIDIDGDGKPDINIDTDESL
                     DSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         4123 a         2116 c         1269 g         5137 t
ORIGIN      
        1 gtatatttat atatataaaa ggatttattt cgaaatagag gttgtattga gaactaaaat
       61 ttagacttta taaatgaatg gaattttcat tgttgaaata ctgaaaatat atttgatttc
      121 atataaaaga aagtgcctca tgaactaagt tcattttgag acactccctt ttttttccaa
      181 gctattatat ctttttttaa attctaatgg agagactcca attttttctt taaatactct
      241 gtaaaaagtt tttgtacttc caaaaccaac ttcttcaacc aaatctacaa tggatatatt
      301 gttattaagt aatatccact ttgctttttc taatctatat tcatttaaaa aagttataaa
      361 tgtttttcct gtattttttt tgaaaaattt agtaaaataa tatacactaa atcctacttc
      421 ataagacaca tcttctaatt ttataggttg tttataattt ttttctacat atttgaatat
      481 attgttcaat ctctctaata tctctttaga gtttatttca tattctactg tattttcttc
      541 aatttctcta ggaatctgtc tatataatat aggaattaaa ttatacattt taccttttat
      601 tgcataacta tatccttcta ttttgtatag gtcttcattg tagatatcta tcaatatatc
      661 gaaaactgac tttcttattt ttgctgacca atctctacta cattttttta tactactgaa
      721 taaattaatt aagctttctt tctcctcatt taataaaatt aaatcattaa aaaaagatat
      781 atcaaattga aatactaatc tttcacttcc cggtgatggc agtatataat gaatatctcc
      841 tccttttatt atcactatct cgcctttttt taattgcata ggcttgtcat ttataaatag
      901 atttactaca ccttctgtta taagtaatat ttctatttcc ttatgccagt gtggtgttgt
      961 taatacttct ccttcatttg atagagtacg aaaaggaaat tcatgtatta atttaggcat
     1021 ttctaaatat attgccatat atatcctctc ctctctagtg tctaaaaatt aatttatact
     1081 atttaaaaga atacacattt tattttaaaa acctcaaatt tataatttat acaataccta
     1141 cacttcataa aatatatcta atatttcttt ttttgtatgt gcatttaaaa gtctttcacc
     1201 tatattttta ccacctaaaa tgtaagctat tttagatatt aatatcatat cttcattgcc
     1261 tccagattgt ggcatagcaa acataattat tacttttaca ggtaagccat ctatacttgg
     1321 ccaatctaca ccttttttag atttacctat tactacagat gccttcttta catattttgt
     1381 ttttccatgt ggcatggcca tttgataacc tatactagta gagcctattt tttctctatg
     1441 ttttaatgcc tttataaact tactttttaa agttatactt cctgtatcgt ataatttatc
     1501 tactaattcc tgtattatgc cttcttttgt agttgatttc aaatctaaaa taatgctttc
     1561 ttctgttaat atttctctaa tttccatttg ttttctccaa tagattattc ttaaataata
     1621 ttttaatatc ctctttgctc ttgcaatcta ttatatagtt tatatagttc ttttgcctat
     1681 ataaatttga tatttccatt aaaatattct ttaaaaaact ttgattacta atttctaaat
     1741 aaatatcaat agttctagta acattattga tattcattat ccctttattt aatttacttt
     1801 gcttatgtat atatatattt aaatcttttc ctaatgaaat agattcatat ttactcccta
     1861 aattcacacc tattaaactt tccatctgag ctacatcaaa attaaaatat atattatctt
     1921 ctaaatacat tcctaataag tttgtatcta agttcatact ttttttaggt ttagaaagta
     1981 aagcattttt tatattatta atatcatttt ccattaatag tactgataca actatatgta
     2041 atttatcttt caaatctaag tttacagtag atattacaaa gtctacatca tctaaatttc
     2101 gcttactcaa ctgatgcata ggaactatat caactatagt ccattcagga aattctcttt
     2161 ttaatctggc tgcaaggagc tgagatgttc catatcctgt atgacaaact actattactc
     2221 ttttactact catatttctt tctattgccg cttgaaaata tgtggcaaga tatgaaactt
     2281 catctatact aatattcttc agattatcat actctgtcat aatatttata gtcatcatac
     2341 ataatcccag cacatctgaa aatcgctctt gtatctctcc taacaacggg ttttttattt
     2401 ggatatcata tcttaatctg tttaacatag gccttacatg agatgctaga cttttttgta
     2461 gtaagtcatc atttttgaga tttatattta aaaattgtga catattctct atcattatag
     2521 atgaaacttt ttcactttca cttaatctat ctgattcatc attgtaacta ctattatttt
     2581 cgctactaaa tccagatgat accaaaaaaa tatatatgta cataagttca tctttcgtta
     2641 tttttactct gtatctattt tctattttat ctataagtgt agtgacattt ttatatacta
     2701 actcgtccaa attatctaca gaaatttctc tttctttctg agattctatt tgattacctt
     2761 cttcgattct ctttaaacat ataagtatat gggtaattaa atttatatag tagggctgat
     2821 ttatagtata tcctaaattg tattctaatt ctgtaattat agtttctaca aatataatag
     2881 aatcaatatc aaataaattt atcaatgcac taaatgtagc agaatctaat cttgttaact
     2941 cagatatatc atgttgttca taattttcat ctaataaatc atccatcatt ttggcaatcg
     3001 atttccttat atccacttct ttacctataa tcttagttcc ttctaaagtt ttattaagtt
     3061 ttaaattgta ttcttttatc catgcctcta tatatttaaa atcatttact atagaagttt
     3121 tacttacata atatttatct gctaactgat ttattgaagt tatattattt gaatttagca
     3181 ataaatactt taaaatctcc attcttctct gctctactga taaagattta ttttccttcg
     3241 attgtatatt taaactatta attaagtcta tgttcttctt tgctaaatct tctattaata
     3301 ttccatagcc tctttttata tcaattttta tattaaaagt ttctaaatat ttctcaatca
     3361 tcttcaaatc tttttgtaaa gttttatcag atacatttaa tttatcttta aagtatttta
     3421 taggtctata tttattttcg tttagtatca atttaagaaa ttcaatttgc ctcgacacaa
     3481 gcatacttag ccctccctac ctatgtttat atatttctat gtttatatta agtactattt
     3541 taacatattt actaactatg taaaattact aagatacttc tgtatcataa aagaatttat
     3601 gatacacctt actaaattga tataataaga taatataatt tcaaaataaa aattaaggat
     3661 atttagaagt agttaactga ttctaaatat ccttattatt attctatata cttgttgtta
     3721 ttccattaat tatttttact attatttata atttaagcac caagtaattc aacttcacta
     3781 ttttcactcc ttatagtagg ctttgttacc cctataatca atgctgttat tactgtacca
     3841 atcaatatac aagctatata tgcaactggt gcactacatg caaatggtat tacaaatatt
     3901 cccccatgtg gtgccattaa agttatatct aaactcatac atattgcccc acctatacaa
     3961 gaaccaatta tatttgctgg aatcattctt aatgggtctg cagctgcaaa cggtattgcg
     4021 ccttctgtta tgaaagatgc ccccattata gcagttggta gagatgactt tctttcttct
     4081 ttagtaaact tatttttgaa tattaacata gctaatgata ttgctaatgg aggtaccatt
     4141 cctcctacca ttaatgctgc tgatggttca aatatattac ttgaaaacat agccaatcca
     4201 aaagctgaag ctgttttatt aataggtcct cccatatctg atgccatcat ccctgctaat
     4261 attgcaccta ataatacttt attagttcca gacatacttt gtaaccaaga ttgtaaagct
     4321 tcatttaacc atgatgctgg cgtattaact acaaagaaca ttatagctcc actcgtaaat
     4381 gcaatcaaaa gtggacatat caatacagca tttactcctt ttaatacatc tggtaaattt
     4441 tctgttatct tcattactcc taaagctata tatcctgttg caaaaccagc taccatacca
     4501 cctataaaac ctgaacctcc aataactgcc ataaaaccag ctatcatagc aggagcaaat
     4561 ccttgtcttc ctgctattga tttagctata aaaccagcaa gtatcggtat aaataactta
     4621 aatgctgaat cgcctccaag ttgtccaaag aatccagcta tagggttgta tgtttcatga
     4681 gttgggtctg atgcaaatat accaaacata aaagctatgg ctataccaat tccacctatc
     4741 actacaaatg gaatcatttc agatacacca ctcattaagt gcttataaac accctttttc
     4801 tctgaccctt gaagcatttc ttttgaatct tctaaagtct ttccacctgg tttaatttct
     4861 ttagccattc cattttcagc gtttataatt aaatttttag ctaatgaaac attcttagca
     4921 tctactactt tcttttgtag cactttcttt cctataaatc tactcagttg aacttcttta
     4981 tcacaagcta ttattacgta ttcagctctt tttatatctt ctttagtcaa cttattttct
     5041 acacctatag ctccatgagt ttctatttta acttcatatc caagttcttg acctgcttcc
     5101 tctaatgctt tttttgccat aaaagtatga gctatacctg ttggacatcc tgttatccca
     5161 actataagac ctttattttg attattacta aaatctcctg attctgcagc tttattatta
     5221 aatatactta atatttcgta tggagtttct gcacttagta gatgcttaag aaaattttct
     5281 tccattagat atgtagctag tttagatata atcttaagat gttcatctga acctgattct
     5341 ggaactgcta tcataaaaaa tgacctaagt ttttgaccat cattatctgt aatctctcta
     5401 tctgttctta aatacatcat tacaggttga tttactacat ttgacttagc atgaggtata
     5461 gctattaaat tacccatatt agttgatgat tctttttcac gtttattaat atcagctata
     5521 aacttttctt tattatttat ataatttcca tggtacaaat gatttgccat ataattaatt
     5581 acatcttttt ttgtttgtaa ttgtttatct agtacaatac aatcgcttcg tagtatttta
     5641 cctatcataa attctcccct ccttttaatt tgttacctca taattaattt ttatttttat
     5701 aaaagcactt taacttatct atagcattga atagattcat tttaaactct ataacttctc
     5761 ttgcagcttt tatacatggt ggatatatag tatctggctc atataagctt gggttttgtt
     5821 ctaatagttc tctgcacttc ttataatatg ccgacttaac atcactcgaa atatttattt
     5881 tacaaactcc tagtttaact gcttgtgcta tttcttcatc tggatttgat gaaccaccat
     5941 gaagaactaa aggtatatct actacttctc ttatttcttt taataaatct agtttaagct
     6001 ctggtttaaa accttttgga tatataccat gtgctgtacc aattgcaata gctagtgttt
     6061 ctactcctgt tttttcaaca aaatccttag ctaattttga atctgtatat attatttcat
     6121 cagtaccacc ttcacttgat gttcctgttg ttcctattgt tccaagttct ccttctacgg
     6181 acacatttaa tccttttgct atttctacta tttccctaga tatagctaca ttctcttcat
     6241 atggtaaatg tgagccatca atcattactg atgtaaatcc acatcttatt gcctttaata
     6301 tttgttcttt attttcccca tgatctaagt gtattaccat aggtacttta ctcttatttg
     6361 cttcttctat gcaactagct acaaagctat ctcctagaaa acttagctca tttgggtgta
     6421 ttgcaagtat tactggtgca tttttttcgt tagcactctg tactactgct ttaagtattt
     6481 gcccacttcc tatattaaat gctggtacag caaattgatt ttcttgtgcc acttttagca
     6541 tttccttcat atttattaac atattgtacc tcccgatatt ttaaatttta ggttgaattt
     6601 gcttcttgtt aattttgttg ttgtttatat ttaaataata tcagggtaat gaagctcaga
     6661 aaataacaat cgttttcctt agtgaaaagg aacttttatt atacactcta agttcctttt
     6721 cactaaataa tttttagaag tctgcatcat agttaattat atttctcatg tacatcttct
     6781 ctttttaagt gttttatgct ttagccttcc tagattttat aataaacaat taagatacat
     6841 taactataaa tttaaagata ctcatgcaat agttaatgct atattagagt caaaaaaatt
     6901 aaaaagatat tagctatttg gccaatacct tttcttggat tccaatagta tatacgattt
     6961 atagaaatct tactcacgat tatctaatgt tttacttcca taaactttgc ataaaacacc
     7021 aaagtgacac cctccaaatt catctctagc taataaagtg atttggtttt ctaagcacca
     7081 atttataaat tagcctttgt tatcttatgt aaatcttttt ctatctaatt taaaaattat
     7141 tcctaatgct atatagcata taccaattac taatacacca taagccatag atactcctat
     7201 cttaaattct tgcatattaa ttacaataga aactgaaata ggtttattac aaacaccata
     7261 aagcaaggct gacattgtat attatccaat tgtatgaatg aatactaata tagctccttt
     7321 taattaaatg atgctatata aatcatattt atcccaattt ttagcctaac ttctttatat
     7381 tcctttttaa taaaaaaaga tattgactaa atagtcaata tcttttagtt gatttaaata
     7441 cccacaattt taatctaatt tttaagtgtt tccttaacat aatcttataa atattaaaat
     7501 ttatctacct ataaaatttc tccattaaat tttattacat tttatcttaa aacaattata
     7561 atctgacctt aatcatatat tatataaata tttttaataa acttaataat taaataacta
     7621 tatttcatta tccatcttat aaatgtttat aaactttcca ctattcttct tcaataaatt
     7681 caaacaaatt ttctactgta gtatcgaata ctttagcgat atccatagct aacttcaaag
     7741 aaggattata tttcccattt tctaagtgta caatagtctc acgtcttacc ccaaccaatt
     7801 tagcaagttc attttgcgtc ataaggagtt tttctctgta ttctttaatt tttgttttca
     7861 tcaatattgc cacttagtta catatccccc ttttatcaaa gtatatatac aacactaatt
     7921 tagataaaga ctgtataaat aaaactgcaa tgaggattat acctgtatct aggtttgata
     7981 tatttatatt acttgtataa ggtgttgcaa agaaaataga tattattact atagaatata
     8041 tcattatttt tattgcaatc ttattaacct ttgatagtat aaattcagat gattcatcaa
     8101 ctaaatcatt atatttttta ttataaattg aataacatcc aaaaactatt aaccaactca
     8161 accctggaag agaagcagtg cttggacctt taaaaatata cttagtggct aatacagcta
     8221 taaaaaaata agttattaca atcaactcca ggataagctt tgatcttaca ccagtttctg
     8281 atttagacat ataaaatcac tccttaaaaa ttatattctc tatactcttt aattttcgtt
     8341 tttatcaata ttatcactta gttgcatatt ccctttctat caaaatgtat atacaaaatt
     8401 aatttagata aagattgtat aaataaaata gctaagagaa ttacacctat atccaaattt
     8461 gatatatcca aacctcttgt ataatttgct gaaaagaaaa cagatattag caaaatagaa
     8521 tataacatta tttttgatgc aatatgatca acctttaata acataagttt tgacgcctcg
     8581 tcaactaaat cattatattt tttattatac atgaagtcac atgcccaaaa agcgaataaa
     8641 attataaaag taatacaaag atttccctct ttaaaaataa acttttttaa taatgagtct
     8701 ataaaaaaat aggatatcat aagtaactct gaaacaagat ttgttttaac accaagctct
     8761 gatttagaca tataaaatca ccccttaaaa gttatatatt tataacacct tatgttataa
     8821 atatataact tttccaaata aattgcaata gaatattgaa aatattcaat aatatcttca
     8881 taaatatatt ttataaaaga ttttataata aacattacaa tacagtacta aattagttta
     8941 tttcatctat catctcaatc atatcattta ctaactatta ttctaatgag catataacat
     9001 tgaaacttat aattatacaa cctaaatgcc tcccaatgta ccctcaaaaa catcatttcc
     9061 tgtaactgaa gtataattgg tatattttat cactgaaata aaaaagcact agtcaataaa
     9121 gactagtatt ttcaagtatt ttatcattct ttttttaatt attggagacg aatcatcaga
     9181 ataatgccca taatttatct atatgcaaat tctaaattta aaataaagtt tcagataaat
     9241 ttactattct atgctatgat acaacagaaa aaactgaaat ctatgagtgg tagcttcata
     9301 taatcctagt tcctacagta atcaaaactg aggtttattc tatatgtaac taatgataaa
     9361 ttttattata ggagttatta gctctatttg tgttactata agtagtaatt atatatatga
     9421 taatttttta acacttttct gttggctata tagaaagtgg tttagaattt aaatttaaat
     9481 aaaatttaaa ttcaccaaaa agaaatctat attagaaacc actactctat tccaccagat
     9541 tgtaattttt tgttctttat atcacagtgc tataccatat taaattgaaa aataagtttt
     9601 aaatttattt gttacaattc attaattcaa aaaaatattt tgagtcatga gttttgtaaa
     9661 gatttagcca tgaatttaat gcttctgatt caaaaacatc taaaagtttc aatacttgaa
     9721 acgtaaattc caacggagca attcctgaag ccgtaactaa attttcatca ctaactacag
     9781 attccgtttt ataatatttt tctccagagt aatttggtat taccatttta agaaactcta
     9841 agtcattact tgtatgattt ctaaaatcta atagcccttt tttagctagg cctaatgtag
     9901 ctccacatat ggctgcaact atagaatctt gtattaaagc ttctttaact ttttctaata
     9961 ttatctcatg attcatggat aaccaagtat ttccccctgg caaaattaat aaatccttac
    10021 tttccatatt aaactcatca actgtaattt caggtaaaac tcttaaccca cccattgtta
    10081 taataggatt tttatcaatt cctacagtaa ttacttttag tggttttaat ccttttttaa
    10141 aatatcttcc tgtatttaat tcagcagtta aatatgccac ttcccaatct gacatagtat
    10201 caaatacata catatatact tttctatttt tcatcttttt tactccttat gtaattaatt
    10261 tatatatcta attataaaat aacttaactg acatattcag tcagttaagc tattaaattt
    10321 gataataatc aattaattct agtaaatctt caattatttt cttcctaagg cttagtggtt
    10381 caattacttg tattgtcttt ctataaggta aaagtatatt tggtatgtac ttgcatatta
    10441 cttcctcttc aagcataaat attattttat taggtgttct ttctttcaag tagtgcccta
    10501 aaaaccaatg ttggcttaaa ctatctaaag aatctatact cccttctaca actaattgaa
    10561 ttatattttg ttcatctttt atttcaggta taattccttt tataaaaaat tcacttgctg
    10621 aaaattctgt cggtctgtta aatgtatttg atgtttctat gatttcacta atcctatcca
    10681 ctctgaaact acgtacctca tttctcatat tacaaaatgc aactacgtac caattattat
    10741 tccagtgaac aattccatat ggatttataa ttctatattt tgattttgct ttacgacctg
    10801 catgatagtt gatttctaca gatagctcct ttaatatact attctccaat tttttaagta
    10861 ctgattctat agacactttc ccaattggtt ttattacttc aagggctctt aagtggtttg
    10921 ttaaaatatc ttcttgtttt tgatttgaat acatttttaa ctttaatatc gatttatcta
    10981 atgcttcgtc taagaagtat ccttcttttt tagcaaatag tgctgcatga agtagagatt
    11041 tttgctcttc tatatcaaaa aaaagaggtg ctttagtaaa attatttaaa aggctatacc
    11101 caccattatg acctgtatct gaaattatag gtactccact cgcagaaagt acatcaatat
    11161 atcggtacac tgttcttata tttatttcta atttttcagc tatctgtttt gctgtaattt
    11221 ttttgtcaga gtttaacatc cacagtatag acatcatatt gtcaatttta gccatgtagt
    11281 ttcacctctt tctatttgtt atataaagtt attatataac tttttgattg attttttaca
    11341 aaaaaacatt agtaaactgc taataattta tactaagatt tatttcaaat atttatagat
    11401 agtctttttc tatagttaag ttatccatta aattacatag tagtatatcc attttagttg
    11461 atatattttc agctacctcc cttgaatatt attacaataa gtctaaatct tctttcttcc
    11521 ataaatatat tatgaggaaa gactatcatc tttaatggtt taatagtata aaagatatta
    11581 attttaaaaa atctattttt atatcagatg ggtattgttt tttataaaac tgaaaaaatc
    11641 acctaataga atctatgatt ttttaacata acctccataa attagtacca attatcactt
    11701 caaattcaaa tttttatctg aagaatatac aatcatattt ccatactcaa cataatattg
    11761 agtgtttggc ttgtatttac aaattattat tctaacaaaa acaactatac atagattaaa
    11821 atcatcttct aaacataact actaaaattg aattaatgat atcaattcca ttaatctctt
    11881 ttaataaaaa attctctttt ctatatgaaa attatttcaa agcttttaag ttaaatacaa
    11941 aaatgactat aaatagattt ttatttacac aaatctattt atagtcacta tagtttatcc
    12001 aataaaataa taaaataaat atgtacacaa taaatgttat ttacgtctta agtatttatt
    12061 tgttagattt ttaattaaaa aaaacaagct aatgaacatt aatgctaaat ctagcattat
    12121 attgctttga tctcctgttt gtacattttg acctacatta ctatctaaac tttcatctgt
    12181 atctatattt atatccggtt ttccatctcc atctatatct atatttacat ccggctttcc
    12241 atctccatct atatctatat ttacatccgg ctttccatct ccatctgtat ctatatttat
    12301 atctggtttt ccatctccat ctgtatctat atttatatct ggttttccat ctccatctgt
    12361 atctatattt atatctggtt ttccatcccc atctgtatct atatctggtt tgctatttcc
    12421 tccattatct atatctggtt tgctatttcc tccattatct gggtctggtt tactatttcc
    12481 accgttatct ggatccggtt tgctatttcc tccattatct ggatctggtt taccattgcc
    12541 accgttatct ggatctggtt tgctatttcc tccgttatct ggatccggtt tgctatttcc
    12601 tccactacct gaatccggtt tgctatttcc tccattacct gaatc
//
LOCUS       DAENCV010000107        12625 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAENCV010000107 DAENCV010000000
VERSION     DAENCV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.108"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(66..1946)
                     /locus_tag="KQD82_002946"
     CDS             complement(66..1946)
                     /locus_tag="KQD82_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG4381404.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISIKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTLDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2156..8119)
                     /locus_tag="KQD82_002947"
     CDS             complement(2156..8119)
                     /locus_tag="KQD82_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG4381405.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNIQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNFTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKISFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPINN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIMVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8243..8328)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8500..9903)
                     /locus_tag="KQD82_002948"
     CDS             complement(8500..9903)
                     /locus_tag="KQD82_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG4381406.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10365..12260)
                     /locus_tag="KQD82_002949"
     CDS             complement(10365..12260)
                     /locus_tag="KQD82_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG4381407.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGLSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         3481 a         2330 c         1405 g         5409 t
ORIGIN      
        1 taagaatgca tttaaatata taactttata tctaaataca ttcttatttt ctatttactt
       61 aatatttatt tttcaattaa actcttaatc ttgtctatta cagatgaggc tattcctcca
      121 cctacttcaa acaactttga tgaattttta ccttttaatg acgattcttg tgatttatct
      181 aacgtatttg tagcaagtat aactggtgaa ttgtttttag ctgctaatgg tccaactgct
      241 aaagcatcta taagtttatc ttgatttgca actacaactc catctagctt agattgagag
      301 tagaatttat ctattacttt ggcattcgtc tcttgtctag tttgacctgc caccctattg
      361 tttgatatat ctttgtttgc tatttttcca acttgttcaa tagccttgtt agatattttt
      421 tctactccac caataaaata tatatttgac accttatttg atttaataaa ttgttcagct
      481 tcattatcta caccatcttt ttgtgtaagt actattggag ttttttcttt accagccagt
      541 gatgctattg ataatgaatc agcttctcca tttcctgctc ctatatacaa cttgcttaca
      601 ttattcgtct tatctatttc ttttgctata ttaatagaag tttgatatct tgtatctcca
      661 cctattcttt gtactgatat ttttgaattt acagctttga tagcttcaac aactgaattt
      721 ggcattgagt tgtttccacc tatcacaatt acttttgatg gatttagcct tttcaattct
      781 tctacagttt tttgaggaag cttttcattt gatgataaaa gaattggtga gttttttaca
      841 ctagcaagtg gtgtagctgt aagcccatcc accatacttt tatcttctcc atttactatt
      901 acaactgtat ctgattttgt ccaaccactt tgagatattt tgatagctgt ctcaaatcta
      961 tcagcacctt ttaatttttt gactgtgaca ttagatgtag aatcacttga atttcctcct
     1021 ccagagcttc caccactaga tgagccgcct gaattacttc ctccgccatt atttccacca
     1081 ttgccattat ctggtggtaa ttcatccggt tttgataatt cattttcaac tatgtattct
     1141 aaactttttg gaagtgtaac ttcaacatat ttgtttattg tagcttttct tcccttagtt
     1201 gttttttgtg ctaatccaag ttttaatatt acatttgaac cactttcttg tgtttcttta
     1261 ctaacaatag aatagtttat cctaatattg tttaataaat attcaagtgc ttcacttttc
     1321 tctaccttat acttttcagg gtcgttttta ctagaaccag ttccaccaat cggttttact
     1381 tcactctcat taataagggt aaatattaat ccttcatcag agcttatatt ttcataatcc
     1441 aatgttttgt tgttatatgt tacaattttt aaagctggtt tatcgcttgt tccttttcct
     1501 aaaactttat agtcagcatt tgagttactg ccagattttt ttatagatat tcttcctata
     1561 ttcaatctat ttccgtctag taaatcttca tttgatgtaa catatatatc taatgtagtt
     1621 gtattacttg atttgttgta ttctgctcta caagtagcat ttgcatttcc actttttatc
     1681 tgagatttta tattatcaga ccaagtaatc ttttctttct gctcattatc tactacttca
     1741 acctcacctt gtacttttat actggcttga aatgaatata ctatgttttc tactgagtcc
     1801 acagatatat ctatagtatt tactttctca tacgtttttg ttccttgtaa aactggagct
     1861 gcatatgcac atgtagcact accagttaca gctactgcca tagttgatac tgctatagtt
     1921 cttataaaag attttctttt actcattaac acccgtcctt atctatcatt aactaagtgg
     1981 ataaatttac ttatccacct agcttttata tttaatttat aaattaccct aaacttatag
     2041 ttacatttat atactttact tttaatatct actattttaa aactattcta gtacctaatc
     2101 ttattagata tacaatgtta gtgttagtat ttttacgttt ttagagttcc acctactatt
     2161 tatctctaac aatattaccc tttactttat tgcttacagt tataaccttc ttattactga
     2221 tatcaataga atttcttgta gcttttctag ctttactatt actatcagta agacttattg
     2281 tctttaactc atcataattc ttagctccta caggtacata cattgtatca cttacaaatc
     2341 tttgacctac tggagtatct ccttgaaggt cattgtatct atatagttca gccttaacct
     2401 tatcacttaa agaatcaaag ttttcttttg gcatatagta aacccatgta cctttactga
     2461 tttcacctag ttcacctttt tctggaactt ctgcaaataa aagcgcttct ccagatatag
     2521 tcttattgtt tttatctata agtagtggta aattaaatgc tggatttcca ctgtattcac
     2581 ctgtaataac tattgaaccc gctggaatct tacctgtttc atcttcactt gtgctagagt
     2641 catcactttg atatacataa tcttctgcta attttccaat tccattagta cgagtttcgt
     2701 ctgcattatt cattgtgcca atttctatat tatcacctgg aggtcctatt atatctaatt
     2761 ctgcaacttc tatattagac ttattatttg agattagttt tacatatcta gcttggtatg
     2821 tccaaagttt accatcttta ttaaatgtaa ctctagcttc attatcttgg tcttttcctc
     2881 caccaactaa atcttgtcca tattcaaaag ttccttgact tacatcttcc cagttctttt
     2941 tatcattact tacttgtatt ttgtaattag ttaaaggatt gtatgttgtg ctagtatttt
     3001 ttctaaagtt aaataatcct tttaatgaga acttcctagc ttctgcctct ggttttgtat
     3061 attttagtcc tactataggt aatgtttctt tcatgtctac cattatatat ggattttctt
     3121 ctacatttat atcagggtct ttttgagggt ctttgttcca ctctgcttta cttattatct
     3181 ttcctttata tgtagtactt gcatcattat cttttatatt atcaatttca ggattttgta
     3241 atggtccatg tgagtcattt tcagttctaa catcaccgtc agaaacagta tttgttatga
     3301 atgaccatgc tgatttattt attttccctt catctgataa ttttacagta ccaacttcaa
     3361 attcttctgt tggatttagg tgataatcat atgcaactac cttatatgta aatgctctgt
     3421 tattaagagg agatatatta tcagtaaatt tagtcttact atttcctttt gcatccacaa
     3481 atccaactgt tctgtatttt acctgtgttc cattttcacc atctaaataa tttccatcac
     3541 ttcttattat ttcgtatcct agtatcttat ctttatcctt atctacatct aggtctatag
     3601 atattttatt agatttaact agagaaccac tttgtattcc atcagaaaaa ctagcactta
     3661 ctttagtatc ttttgccata ctagctatat tttcatttaa aatctttctt ctagcttcgt
     3721 catttacata ttgtatcttt cttgattctt tttcgtagtt cttactatcc attatactgt
     3781 ttaattctgg tgtaatttct actccccatg ccttaaagta atctcttaaa tctttttcaa
     3841 caacacttga tgccattgct actagcattt ggtcttttgg taaactatta attgggtcac
     3901 tacttgaaag tgctctgtat tttcttgaaa tctttgcata gaatgtatca ttacttaaat
     3961 cagcatctga gttattcttt aacattgaac ccgtaaaatc atcatcataa gctagatgta
     4021 attgccataa cattccaagc ctttcaaata cattgcttgg tatacctaag ctatttgaag
     4081 ttacatgctc atatatctta tccattgttg aaccttcaag tcttgaatgt gtcttatcat
     4141 caaaggtctg agcaagtaaa gcaagtacat tgttactagt ttcgctatat gtcatctttc
     4201 caatatctgt aacatgtcct atctcatgac ctattcccca tccaaataag tttcctcctg
     4261 tagcttttcc actatcatct atttggaaag gtactccatt cataagccct ggaactgaac
     4321 catattctac tcctacatgt aatccagaag catacataaa tgctccaata aacattcttt
     4381 ggtattttat gttcatacga gattttggag ccatatcatt tttagttaag ttaaattcat
     4441 tatcatctac agttccatta ccatctaaat cttgtgtttt aaatactcct ctttctgagt
     4501 aaactaaatc cattatttgt tcccatgcaa gcatactatt atataatcta tctacttgtg
     4561 tatttaaatc agcatttcct tttgatatcc cttgatatac agctgttgca ggaacattta
     4621 atgttacttt atcactttca atatctgtag tgtttaacac accagttttt tctgaatatc
     4681 catatgaatt accaacattt gttcctggat aatatggttc tccttttaca gcatctttat
     4741 atttagctaa ttcttctata tatgctttta tctttttctt agattcagaa actcccgcag
     4801 gactcttatc atttattgtt ccatttagat taagatgtgg tatcttagta gctcctgcta
     4861 cacgtacttt tatatctcct ccacttggag tatcttcagc gtatcttatg tataaacttc
     4921 ctccacattc tatatctaaa ctatgaagtt tgcttaattg aattacattt tttccagtgt
     4981 taagatttat ttctccactc ttatatgcac cagattctcc atagtactgt gtatatatca
     5041 acttaggagt ttttccactt ttacttccaa catatactgt gatttcatct cctgctctag
     5101 cagctactcc taatgcctgc cagtcatttt gcatacctaa attattggaa tttacagtat
     5161 actgtccatt tttatcttta tattttccta atttatttat ttcttgattt acagtagtta
     5221 catcagtaag tgtggctcta tcattatata agtcttgagc taattttaat tctctctcaa
     5281 taactgtctt atatggatga tattctccac tagctttatc tggagtattt aatctatctt
     5341 ttaattcagt tattttttct tgagttacat cttcttttaa ttcaacttgt aggtcatcca
     5401 taaatagatt tcttacatca tcttctatac tgtcatactc ataaaactta agttctgata
     5461 tattagactt atatgaatag gatgagcaaa cacttacttg tattttggta gcagttattg
     5521 gttcttttaa tctaaccatt acatacttat tattatttat agggtctgtc tttgtttcaa
     5581 aaggaacatc ccccagattg tgtaattgac cattttcatc ccaatatcgt acctcaaagt
     5641 catttattgt cttatcagaa acatcaaaac gaggtgtagc cataaaagta tccatagtat
     5701 aagatttatc aaaagttatt attggtcctc tattcgttcc ctcagctgca ttatatgttg
     5761 ggaatgtcca atgtgttgta aagtccccat ctactacatt gaatttatca aatggatttt
     5821 tatcactata ttcgcttttg tcatatcctg ctggataagt aacatctaca atgtgatttg
     5881 ttggtattcc aaatccacca ttctctttag gagtattaat taatctatag ttaggtgttg
     5941 ttggtgcatc catgctttca gttgtaccta tatatgtttt agacattcca ctagttccca
     6001 agtggttagt agctgtcatt ctaacttcat atgttgtatt atccttaaga ccttctatct
     6061 ttgaacttgt cccacgaata agcttatcat tgtattttat ccaagaccca ttagaacctt
     6121 tttctttata gtacaagtca aagtctttag cttgtttatg atttttccag cttacatcaa
     6181 gagatttaaa tttaccttta acatttatac cttctggtgg ttctggtttt gtgtctggaa
     6241 caacttgaaa ttctaatata ctatctgggc taaattgtat aggtttatag tttgaatcta
     6301 cattttctgg tactccgtct aagttttctg gtgttgcatc tttataacca ctagaccaat
     6361 ctccatttaa agattgtatg ctaaccctat atagtccata tgcttctata tctctaactt
     6421 ctattgtatt tttactagtt tgtaattttt tagtagataa tacttttccc ttactatcaa
     6481 ttttgtctag tataagttcg tatgctgata catttacttc attattccaa taaaaagata
     6541 ttttttcatt tccaacagca gtgctagtcg atattttatt tattttaggt atattcatct
     6601 ctggttttgg tatttcttta tatacattgt ttaaaaattc aacttttgat atttctgcaa
     6661 gatttctatc atttgctgtt gtagctgtta tttttatagt tatctttttt actgctactt
     6721 gtttaccaag atttattact atatcactat tttgaacatc tgcatatgtt tctaatgctg
     6781 acctactata tgtagatgtt gtaaagttat catctttact gatattatcc ccttcttttg
     6841 gagtatcttc atctgaatta ggtttatcca ttgatgaatt ttctttatca ttatttttat
     6901 cttgattggt attagagttt gcttcttgtt tatcgctatt ttcctgttgt ttatcattat
     6961 tttcatcttt attatttgag ttattatcag tctggtttat attttcatct ttattattta
     7021 cttcttcttg atttacttct tcttgttcgt tatttacttc ctcttgttgc tcctcatctt
     7081 ttaagttttt catatattct ttgttttcat aaggaatctg ttgaatatta ccatctttgt
     7141 cagtaacctc aactattcct tttgctggag cagtaccttc tggagctttt atattgatga
     7201 cttccatatt aagtgcttct gataaagcta tatccattac tgctggtttt tcctcagata
     7261 tttcatcatt ttttgaagat attgtaactc cattattttc tccactaaat aaattttcta
     7321 cattaccatt aatatctaca tcactatttc cttttaattc aaccttactt gtatctacta
     7381 taggattggt agatactata ctttctaggc tttttgcatc ttcactttgt cctaaatttt
     7441 tctgtacata atctaagtca acaatatcta cctttccatc tctatttaaa tcaaattctc
     7501 ttttatttga atctatattc tctaaaacct tattataatc actcttatct actttaccgt
     7561 ctccatttac atcacccatt aagaataatt cataatttaa aggtttattt ttattatcta
     7621 cacttgctct attgcttaat aagactcttt gtgaataact tgatatatca atgtctttaa
     7681 tttctttgtt cttaaatcca gctccagaga cttctacatt gtattttcca acaggtaaat
     7741 tattaaatac aatattataa tatgatatat tctcatcttt attttttatt tcacttctac
     7801 ttgcagataa tttttttata gtatattcta tattatctgt cccaacattt actgttcctg
     7861 aatctttttc tccaagcttt attattccac tattgttact atcatctttt aatcgtaact
     7921 gcatattagt catactttct gactcgctat tttttattgg cagtgaaaaa tttaaatcta
     7981 cttcaagact cccccttaac ttttctgtaa ctgatttttc aactgccctt agcttcccct
     8041 ctaatatagg ttctgcaaaa acgtttagtg gtgcactaca catagatact gctagtacac
     8101 aagatattgc ctttttcatg aaataacaat cctcccttaa taaatatcta atttttcatg
     8161 aatttaaata ctataagttt cagacaataa aaaaagacac tcaaagagaa tcttaattat
     8221 aaaataaaaa gactttactc tttttagcaa ctggctgata tggctttaaa agctttaatc
     8281 cctatagttt tgcgtcatta gatttcccta attttgccat ttaattttca ctactttctt
     8341 acccatttta tactatattt aaacttttta atacaatttt tttagattaa attataaaaa
     8401 aataacattt atattaattt attaatataa atgttatttt ccattataat ttaattattt
     8461 atatagcttc cttacaagca taattttgtg tatattttct tattctttat atcttccata
     8521 aaattcatgt gtagcatata gattcttatc tcctgttata gctataccaa atcccattat
     8581 ttcataccct ttatgcaaca tattagttcc ttcttctgga tttgtcttcc actcattaaa
     8641 aataaagttt cctatatcat ctgcatctct atttgtatat acaggtttat ctggaatttt
     8701 atatttaatt atattttcac cttgaactgc tatatatcca ggtttaatct cactccaatc
     8761 taagtatttc aaaaaagtag aatatgaatt ttttccattt atcgtatgag ataaagtctt
     8821 cttatttgcc attaaattag accaatcatt tgctagaccc tctaatctgc ttgctacaga
     8881 taacgctctt actccattct cttttctata ctgctttgta agagcatata ctttaccctt
     8941 tattctctct ttaaatgctc tatcatttgg attgtaatat aaaccttgtt gatttggtga
     9001 agcttgattt atacattgct tgataacatt ctgatttatg cctcctagta cagtcaattt
     9061 gtctgcacca cttaaaatac tcttgttact gccattttca actaaaacta caggagcatt
     9121 ttttgctatt gtagaggcag ccaaagcgtc tgttaattgt aagcctttac ttagataaaa
     9181 ttctttacta tttttataaa actcttgtat aactatctta ttagtttcaa atctatctgt
     9241 gccacccaat ctaatagaat ttgtgctctt aactaattcg tcgctgatta tttcactaga
     9301 accaagcgca taactcttta agcctgtcgt gtcaaatccc acactatttc catctgtaag
     9361 tattataggt actccatctc tagcagctac tggtgatgca cttatcgcat ctgcttctcc
     9421 tgtaaatcca ttggctataa ttactttatc tattggctta atttctttga ttttttcagc
     9481 tatttttata cttgtatcaa atctattttc accaaatatt ctttgtacaa ccttcttttt
     9541 ggatagtaac tgattctcaa catttttact tattgttgat tctgagccta ttatatacac
     9601 agtctttgct ttgtttaatc tttccatagt tgtgtttgga atctggtttt gttttgtcat
     9661 caaaataggc gcatttaagg ctccagctaa accacttgca cttagaccat ctgctaagct
     9721 attatctgag ttgattagta ttgctgtatt gtagttttgc ttatctgcta tttttgctgc
     9781 tgtctcatat ctgtcattcc cttttatttg ctcaactgat gtcaacgcat ttaccatagg
     9841 agtatttacc attactaaaa atagtgtaag tcctatagct aatatctttt tactattctt
     9901 catattattt tctaccccca atgtactttt catatttttt attagtactc atacttattt
     9961 ttaatcaaaa gttacaattt tgttaccata ttttatatat gataccatag ttatccatac
    10021 aactcaaaca atatacctaa agtttttatt ctctataaat tcaaaacatt ataatttatt
    10081 ttatttaatt aagttataag ttcaaaataa tttatgttag taatatgttt aatcattata
    10141 agaagtatct ttttataata atttttgcaa tatgaatttc tcaacattat aatcaaaaaa
    10201 taaaataagg tatctcaatc taattctaaa tgagacacct tatttttatt attttatctt
    10261 gttatttgta tattttataa tatctacact atgtcttaca tattaacgca ttgtataatc
    10321 atatattatc taaataaact tttaatatta attcctatat cttattagtt cttagtaaat
    10381 aagtttattt tttctgctat tttttcaata gcgtttttag ctattccttc acctatttgt
    10441 actaacttag tagcattctt aagtcttaac tcaactactt cttcttgtga tttagaaagt
    10501 tcttctgtag ttaatactat tggagccttt gaactagctg caagtggtgc tgctgtaagt
    10561 gcatcaacta attgtccatt tccacctaca taaccatctt ttgctacata tgctttagtt
    10621 gcacctgcat agtatttatt gattacttta gcattagtat cttttctatt ttctccagct
    10681 aatctttcaa ctttattatt taaatctata tcaactaatt tgtcttcaac ttcttttgat
    10741 acagaatttt ctccacccac tatttctaat tcttttcctt ttaataattt ttcagcattt
    10801 gcatctaatt cgttacctgt aactattatt ggagcaccaa attgagcagc tctagctgat
    10861 atactcatag catcagcttc tccatttcca cctactacaa aagcagtctt aaatgtatct
    10921 tttaattgtt ttgctatttc taaagaagtt tcaaatctat cttctccaga aactctctca
    10981 actttactta aacctaattt agaaagtttt tcttcagttt cttttgatac tttagattct
    11041 ccacctacta tatatatttt cttagaagat agattacttc caagtactct taatatttca
    11101 gcttctattt ctgatggtag tgaatctttt ttagataata atattggtgc atttttttga
    11161 gaggcaagtg gtgctgatgc taatccatca actatagcat cttctccaac taaaattata
    11221 gcttctgctt ctccatcttt gaatctctct ttactaactt ctactgctgt tgtaaatctt
    11281 gaatcaccag ataaaacttc tacttttaat tcttttccac cttttacatc agttaatact
    11341 ttgtgtaagt ctctaactaa cttatggttt ttacttgaaa cacttattgt ttctggcttt
    11401 tcatttgcct tgcttaaaac tatatcaaag cttgattttt ctggtttatc tttatcatca
    11461 ctatcttctt gttcttgagt aaggattgct tcattttcaa atgttaaagc atatccattt
    11521 ttaactttat taactatttc tcttcctaat gtagttaatc taattccatc aaataattct
    11581 tctgctgata tgtcttgtaa atctgcgttt ttaactgtta tcgtatgtaa ttcttttgaa
    11641 tctatgtcac tataactctt ttcataacct tcaaaagtac cttcttctga tgttatcact
    11701 tttgagaaat ctaaatgttg gtctcctaca tttaatgtaa taacatttgc tgaatctttt
    11761 gctcttttta cttcaactgt atttgtagat tttatagttg ctgttaactt gttatcagaa
    11821 tcttctgcta aagttgcatt gtaagcatta actgcatcta ctatttcttg agcagttttg
    11881 tagttttcaa ttttatagtc aactactttt ccacctaaca cttgatgtcc tttatcttga
    11941 atagtaactt ttaaactttg cccttctgtt aaagagttta tcttattttg taattgagtt
    12001 gcattagtta agtttacatt gtctactttt atatcgtaaa ctctatcatt tacatttgct
    12061 cctgcttttg tatcttcaaa cttaacttct aatgcttttc taacctcttc gattagttta
    12121 gcagaatctt tcgcacttac tgtgtaattt ttatcaacat tttctgtcac agtattgtct
    12181 gcgaatattg gtgctgctgc actcataact gtagcacctg ccattactat tgccatactt
    12241 ctctttttgt tgcttaacat atttatgtcc tccgaatatt attttttatt ttttatattc
    12301 gtatttccca cacataaata atattatata taatattatt tttcaatata ttttatgtgt
    12361 ttacataatt tactaacaca aacactaata atatcttctt gaaccccttc cccctactta
    12421 gctctctata attatgctca aagatagaaa tccctatttt atagtgattt tctactaaac
    12481 aatcatatac taacaaaagt attcaaactc catttttata tactactatg cactaatttt
    12541 tgctttactc ttattactta aatatatatc actaaattaa tatctatctc catatattaa
    12601 aatatataac atattataaa aatca
//
LOCUS       DAENCV010000108        12433 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAENCV010000108 DAENCV010000000
VERSION     DAENCV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.109"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..321
                     /locus_tag="KQD82_002950"
     CDS             <1..321
                     /locus_tag="KQD82_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4381408.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="KQD82_002951"
     CDS             337..1920
                     /locus_tag="KQD82_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG4381409.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="KQD82_002952"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="KQD82_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG4381410.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEIAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIISGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="KQD82_002953"
     CDS             3475..4827
                     /locus_tag="KQD82_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG4381411.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="KQD82_002954"
     CDS             5019..6128
                     /locus_tag="KQD82_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381412.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="KQD82_002955"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="KQD82_002955"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG4381413.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQDKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="KQD82_002956"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="KQD82_002956"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG4381414.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLVGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="KQD82_002957"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="KQD82_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG4381415.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4981 a         1432 c         2513 g         3507 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttatga gaactttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatag ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aagtggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggattaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagataa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat ccagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaatct
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagttggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcagca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaaaggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggctca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta ggacacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagag gatggagtaa taatctattc
     8881 aacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacaggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagtgca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagtcaac tctgttccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagct
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatataaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaacataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataataga atatggaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaag taactagaaa taaaattatt tatagtaata
    12421 aatattagaa tga
//
LOCUS       DAENCV010000109        12413 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAENCV010000109 DAENCV010000000
VERSION     DAENCV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.110"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            347..1282
                     /locus_tag="KQD82_002958"
     CDS             347..1282
                     /locus_tag="KQD82_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG4381416.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1374..3245)
                     /locus_tag="KQD82_002959"
     CDS             complement(1374..3245)
                     /locus_tag="KQD82_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG4381417.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3435..4361
                     /locus_tag="KQD82_002960"
     CDS             3435..4361
                     /locus_tag="KQD82_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG4381418.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDEHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4500..4646
                     /locus_tag="KQD82_002961"
     CDS             4500..4646
                     /locus_tag="KQD82_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381419.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4751..5275)
                     /locus_tag="KQD82_002962"
     CDS             complement(4751..5275)
                     /locus_tag="KQD82_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381420.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGGKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5328..5552)
                     /locus_tag="KQD82_002963"
     CDS             complement(5328..5552)
                     /locus_tag="KQD82_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381421.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5749..6873
                     /locus_tag="KQD82_002964"
     CDS             5749..6873
                     /locus_tag="KQD82_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG4381422.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFIVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7347..8171)
                     /locus_tag="KQD82_002965"
     CDS             complement(7347..8171)
                     /locus_tag="KQD82_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381423.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8519..9574)
                     /gene="ispG"
                     /locus_tag="KQD82_002966"
                     /gene_synonym="gcpE"
     CDS             complement(8519..9574)
                     /gene="ispG"
                     /locus_tag="KQD82_002966"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG4381424.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9756..10760)
                     /gene="rseP"
                     /locus_tag="KQD82_002967"
     CDS             complement(9756..10760)
                     /gene="rseP"
                     /locus_tag="KQD82_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG4381425.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10773..11927)
                     /locus_tag="KQD82_002968"
     CDS             complement(10773..11927)
                     /locus_tag="KQD82_002968"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG4381426.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12091..12294)
                     /locus_tag="KQD82_002969"
     CDS             complement(12091..12294)
                     /locus_tag="KQD82_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381427.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         4132 a         2091 c         1293 g         4897 t
ORIGIN      
        1 tatatattct ttactcaaat taaatataac ttatatactt tatgtgtaaa ttgtggtaaa
       61 aatattattt aatcaaatca aaacttgatt tatatacctg gaaaaggtat tatattaaag
      121 agtgcttaaa atacgcattt cataaatagc tatatataaa ataataaata aggagagtga
      181 tgggcaatgg atactagtcc gttgcggaga gaatttttta cattttcatc acttaataat
      241 atctaaaatc aaaactaata catatattca gttatataaa ttagttaatt aatagatact
      301 attaaatgat gagatttata taaaactgaa taaaggatgt gttgccatga taagatatta
      361 caaaactata gattcaaaat tagagaaact tagttttttt gaagatggct gttggataaa
      421 tcttgttgaa cctaatcata gtgaaatcaa cgaaatttct aatttattaa atatagatgt
      481 agaaagcata gaatctgcac tagatgaaga agaacgctca agaatagatg ttgaagacaa
      541 tcatactctt atacttatag atataccagt agacgaaagt gactctaatt cttctcatta
      601 tactacaata cccttaggta ttatactaac tgaagaagct attgtaacag tttgtgatgc
      661 acaaactaag attttaaatg actttatagt tggtcatatt aaagattttt ttacgtttaa
      721 aaagactcgc tttttgcttc aaattcttca caaaaatgca gcttattact tgcattactt
      781 aagaaaaata aataaaatga caatcataat agagcgagaa atttacaaat caatgaaaaa
      841 caaagaattg gttcaattat tggaattaga aaaatctttg gtatacttct ctacctcact
      901 aaaatctaat gaacttgttt taaacaaaat ggttagaact gctggtataa agaagtatcc
      961 agatgatgaa gacctattag aagatgttat agttgaaaat agacaggctc ttgatatggc
     1021 taaaatatat ggagatatat tgagtagaat tatggatgct ttcagtgcaa ttataagtaa
     1081 taatcagaat aatgttatgc aaattttaac agtagttaca ctaattttct caatacctac
     1141 aataatatca ggattctttg gtatgaatgt catcaacatg ccattttcta ataacccaaa
     1201 cggattttgg ataatacttc ttattacctc tataatatgt atagtgatta catttttcat
     1261 gtcaagaaat aagctattat aaaatattaa taaaaaagcc attagaatta attgatttac
     1321 atttatccta atggctttta tatatttaaa tctatataat cctataatac tttttaacta
     1381 tcatgttctt catctataat catttcagtt tcaagccctt ctagcattga atctaaactt
     1441 tcttttatat ttagtatata aattggtaca tactcttctt catctattac ctcttcttgc
     1501 aacttaaatg gaggaattaa tacttcatca aagtctatat ttaaattagg aaagccttct
     1561 actatctctt cacaattatc gtactcttca tttgctaaag aaacgtacat atctaggctc
     1621 tttttataat aaagacctgc aagcatatac tcacacttct tacttccata tttaatacct
     1681 tctttataat atttctcagc ttctgcgtca tcatttaatt ttaaataaat ttgacctaaa
     1741 ttatatattg attcaacaca accttgtgct ctagcctttt cataccaaaa tttagaattt
     1801 acataatctt tatagaacat atcataaatt aatcctacca tatgttgagc atacacatta
     1861 ttttcattgg caggtgtatc aaacatcatt ttagactctt ccaatttacc ttctctaaaa
     1921 taaattctac ctagatgtat acgagcattc atatggtttt tgctacttgc catttcaaaa
     1981 tactttctag cattttttgt atttcctaag ctctcatata tacaccccaa tctataacta
     2041 gacctcgtat ctccattttc agatgctttc ttataccaac ttatggcttc ttcaatatta
     2101 tttaaatctt catagatatt tcctaatttg atttgacaag atacattatt tggaatcctc
     2161 gaataatacg aaatcgcttt ttctatatct tgattctgat aatataaatc ccctagattt
     2221 tcaagggaac ttttgcatcc aacagctata gcatcctcat aatatttaat agcattttca
     2281 taatcttttt cttcaaaata tatacctgcc agattatttt ttgcctctgt tatatcatct
     2341 tcagcagcca ttttataata agtttttgcc ttctctatct cattttctct attatataaa
     2401 ttagcaagtc tatacttaga aagtgtatga tttttttcta ttgctttctc ataataataa
     2461 attgcatctc ccagctcttc ttttctatca tatacatatg caagcctata ctcagacttc
     2521 acatcatctc tatcagcact ctttttataa tacttctctg catcttctaa attatcattt
     2581 atttccataa tcatacccaa gttaaagcaa cctttcatat gacctgactt gtacaactgt
     2641 ttatataaac ttactgttga tttattatct ttcttttttc catttaatat tgctaaatta
     2701 tattttgcat catcatctaa aagcacagaa ccttttttat agtattcata cgcttcatca
     2761 tctttctcca aaaaagtata taatacccct aaattaaaac aagctttacc taaattcttg
     2821 tctgctgctg tttttaacca tttttcacac cttacaaaat ctttcatttt aaaatggcat
     2881 acacctaaat tattttgtgc ttgtaaataa cctaaatttg ctatcttttc ataccaatat
     2941 atagcatatt catagccatc taacttatag tacatattac ctaaatcaaa agcagcatct
     3001 tcacttccac ttttatatgc cattgaatac cacttttctg cttctttaaa ttgtttttta
     3061 ctttcatata tatttcctaa agtatattga gattttacgt aattatccaa tgcagcactc
     3121 ttatacaact ctattgcgct ttccatctta cccatctttt caaggagggc acccttagta
     3181 tgtacgactt tcgggtcatt agatatattt tttatttttt ttatagaaaa ttttgtattt
     3241 ttcattattc gctacctacc cattttataa aatatattct ctcaaacatt attatacatt
     3301 ataatctaca attaatgcta taatattgaa tttttacctt ttgtcactat attgtaacat
     3361 taaattttaa attttataat tattagattt tattatgtaa ttggtatata atgtatatat
     3421 aggggggaca ttttatgaat ttcagcaata tttttatcca agttgcagtt ttatttataa
     3481 ttattttagt tggatatttt gtcagaaaat tcaacttatt agatgagcac tgcacatcta
     3541 agctttcaac tttgacaatg acagtttttt taccatcaat gataattagc tccatgcaaa
     3601 ttaactttga tagtaaaatg atacaaaaaa tactattgtt attatttaca tctttgatta
     3661 tgtatatagt ttcaattata attgcatttt tattgaaata cattttaaaa tgtaatgata
     3721 aaaaagattt gggaatatat cagtatatag ttgttttttc aaatgttgct tttatgggtt
     3781 atcctgtaat tgaagcagtt ttaggtcatg aagcaatatt ctatactgca atattcaatt
     3841 tgccttttaa cttattttct ttcactttag gaatttatct tttatctaaa ggcagtacaa
     3901 gtagaggttt ttcaataaaa tcacttataa gccctgctac aatagctatt gttattggac
     3961 tttttttatt tgtaacagga cttaggttac cccaatttat aaacgagcca cttgagatgt
     4021 tgggaagtat aactacacca atatccatga ttatcattgg aagcttactt gctaattctt
     4081 cagctctcga ttgttttgtc aacaaaaaat tatatatagt cacttttata agattattgg
     4141 tacttcctgt gattgtgtat tttattctaa aaggatggat aaatgataag atgattttag
     4201 ctataccagt agtaatttct tcaatgcctg ctgctgcaaa tactgctata atggctaatc
     4261 aatatgactc caatataaca ctagcttcac aatgtgtgtt ttttacaaca ctattttcag
     4321 ttatatctat accttttata agtattttac tcttatcttg atttattttt ttatgtatta
     4381 tataataaca tattcaataa attacaaata taaacatttt attgtagtat attgatggaa
     4441 ttttttccta gatatttgtc aataatatct atatgtatta tagaatagga ggcttaaata
     4501 tggatatgga tttactcttt aaaatagtat ttgctatagt tgctataggt gtttctgtaa
     4561 aaatcattaa aactgttgct agtttagctt ttaaaatagc gttgatattg ctaataattt
     4621 tatttgtata tagaatgttt ttgtaaacat ttaaaaataa gttttttaac acaatagaga
     4681 agtaaaatag attatcttta atgtaaattt tttattaaag acactctatt ttacttctct
     4741 attagcttct ttaaattaaa tttttattat atcttcccat aatataaaca tcttcaaatt
     4801 ttccatctct gattacagca tattttttag ttccctctat ttcaaaacct agctttttat
     4861 ataaatttaa tcccttttca ttatccacaa ttacttctaa tcctactcta gttaacataa
     4921 tccaattatc tgctatatct aaaagttgtt ccataagctt cttacctact cctcttccct
     4981 gataattctc tgcaacactt attcctattt ctgcgctatg tctcaatcgt ggagaagaat
     5041 ttacatgtaa cccagcaaaa cctattacat cttttcctcc attattatta gtttcagcta
     5101 caaacatata atcattttct cccaaagaag ctaacatagc ttcagtttta tcgactctgt
     5161 cactcattaa tgccaaagta tttctcataa cactgtatgt tcttctaatt tcattaaaag
     5221 cttgtgcatc ttctcttctt ataggtctta ttgttatatc tataggctta ctcatattag
     5281 attccccctc aaacataatc tttattttta attaaaattt tacatcttta ttttatactg
     5341 ttcaaatcta tgtatgcttc tacctgaggt acatatatca tattatcttt tatatgtgta
     5401 cttccaaatt cttttaattc tgtaccttct ttatccttag ttattttata tctaatggat
     5461 tccatacctt caactacttc agcatgaaaa aacatacctt catattgttt aaatctaaaa
     5521 ttaaaattgt ctttagtaag ttttattatc atatttttat ctactcctta tataattgat
     5581 atttttctta ttattagttg tatatatact gcttattcat attaatatat tataacttat
     5641 catttattta gtataaagtt ataaatagat atatttatac ttattttaca atctttcttc
     5701 aattgatata taatatgttt atataaacta atgtatgtga ggtgaaatat gagcaaaaaa
     5761 acaaaatatt tttctttaat tctctttcct attgctttgc ttttaaattt tatagccagt
     5821 aaagttccaa atattgttga aaaatattac tcccaattca tagataaaat aattgtacaa
     5881 atattgagta aagtttctgg aattttccca ttttctctct atgagattac aatgtacata
     5941 atagttatat ctatcttctt atttttatgt tgtactttat ctacaatttt taataagaaa
     6001 aaaaaattaa acatatattt aaaaaattcc attttaaata tattatccat tgtatcaata
     6061 gtctactttc tttttatagt gctttgggga ataaactata ataggattcc actagaaacc
     6121 acacttataa ataactataa tttaaaaaac aatacctcta tccaatcaaa acaatatagt
     6181 gttaaagagc ttactaaatt atataaattc cttgtaatta aagcaaatga aactagaaaa
     6241 ctcacattac aagataaaaa tggtattgta aaatctaata cagattataa tggtgtcata
     6301 aatagagctc aactaggata cgataatatc ttggatatat tacctagtgt tagtggtagt
     6361 tattctaagc ctaagtatat tatttcatca aatctaatgt gctatacagg aataactggt
     6421 atatactttc cttttacagg tgaggcaaat gtaaatatag caatacctga cttatatatt
     6481 ccttgtacag ttgaacatga aatggcacat caaaggggat ttgctagtga agatgaggct
     6541 aattttatag cgtatcttac ttctataaaa caccccaata ttgattttaa ctattctgga
     6601 tatattttag ctttaaatta tactgcttct gctttaagta aggttgatta taatgcatat
     6661 gttgatattt ctgctggcat ttcagattct gtccgtagag atttaaaaaa tgaaagtgaa
     6721 ttttggcaaa aatatgaagg taaaattaat gaaatttcta atgagtttaa caattcttac
     6781 ttaaaagcta atggcgttac tgaaggtact cagagttatg gtaaaatggt tgatttattg
     6841 ctaacttatt atgaattata tccatataac taaacactac aattcaaaat tttattcata
     6901 tacagctgat attaaatcag ctgttttcta tttaaataaa ttatttaact tcaagcaatc
     6961 ttatataatt tgatagtact tatactatct gtataacatt tcaattctaa ctattttagt
     7021 taaaacattt ctattatctt ttttaataaa tgttttaact aaaattttta aaacataaaa
     7081 cttccacatt atttcactat ctatataaaa atctaaaaat tcacatataa aattacataa
     7141 ctaaaaaatc ataaatttat agaaaacata tattcaaaat aaaaaagcat gtatcaaaac
     7201 ttgacacatg cttataatct tatgtatata tatcattttg tcacaataaa aatttacttg
     7261 taacactgat atttttaaac aaaatagtcc ttatttaatt aaattaacat taattaaatt
     7321 cgtactcatt ttgctatcat taaacattac ttatagtaat tttctaatac actatttgaa
     7381 acttccttcc cttgaaaact tttcaaccaa ttctcatatg ctgagttttt ccatacacta
     7441 ccatcattaa atgttacttg ttttacacaa gatataatgt actttacacc atgctctcca
     7501 gttgattgtt tccatttatc aaacaataga aaggcatcat tttgattctt acctggtgct
     7561 attgcatttt gctttgcata ttctttctcc atttgtttcc attctttcaa ccaattttct
     7621 acccatgcct tgccttgttg tttttcaaac atacttatta tatcctgtgg tggtgattgt
     7681 tgcatttttc tataagtatg tgaatatgat tttggagaca caggagatat gcctgtaaca
     7741 ataccatagt ctacaccagc tgggctaaat ccaacacttc ctacttcgcc atctgctgcc
     7801 acattttgag catcccaata tagctcaagt ggttttccat ttttatcata tgctaagcat
     7861 gtaatttcat aatccataat tgccttttca ctgctatttg ttaagcttat aacattgcta
     7921 ggccaatgat atggagtatc atcatactgt attttgctat tatttataaa taatggaata
     7981 ttctcattat aaacaagacc tttactactt tgaatatctg ataccttctt tgtgacagta
     8041 cttaccgtat ttgctaagta tacatttgtt ttttctgaat tggtcgtact aagagaaaat
     8101 gttgttgtcg ttccagtaat cagcaaagca acaactaaaa taggtaagct agatattttt
     8161 ttatatctca cagttcttca cctctttttc tattaactta taataaaaca atttaccaaa
     8221 acattaaaat ctatgttaac ataatttacc aactgttcta gtaactatat cctatcatag
     8281 ttactatcaa gtttcaatac tattgatgga aattttcact atattaaatc tttttttaat
     8341 tttataaatt tacaatatga tttaactcat acatatatag tcaaattaga atataagttt
     8401 cttacattta atgaagttta taaaataaaa aatgactaga taattaaata ctgtaaataa
     8461 aaacttatta caaaagtatt taaaaatcta gtcaattaat atatatatga attgtatact
     8521 ataatttttc aatctcttct agcaactcat caactaattt gttaccatca atcattctaa
     8581 ctatttcacc ctttttaaat aaaagacctt cacctttacc cccagcaata cctatatctg
     8641 cctctctggc ttcaccaggt ccatttacag cacagcccat aattgcaaca gtgatatttt
     8701 tttccatact accaatcttt tcttcaacct catttacaac actaattaag tctatattac
     8761 atctaccaca tgtagggcat gatataactt taatcttatc atttaaaagt cccaaacttc
     8821 ttaaaatctg tttaccaaca ataacctctt caacagggtc gcctgttaaa gatattctaa
     8881 cagtatctcc aattccttta agaagaagtg ctcctacccc tatagaagac tttattgttc
     8941 ctttgtgaac acttccagat tctgtaattc caatatgaag aggataatca actttttttg
     9001 atattaattc atatgcatca acagttttat aaatatcgga tgattttaga gatataacta
     9061 tattatgaaa atctaagtct tcaagaatct taatatgtcc cattgcactt tcaactaatg
     9121 cttcagcagt tgcagagcca tactttttta aaagttcttt ctctaaagaa cctccattaa
     9181 ctccaattct aatttttaaa tttctttcct tgcatttctc aactaccatc ttaactctct
     9241 ctatacttcc aatattacca ggatttattc ttattccatc cacaccttgt tctatagctt
     9301 caagggctag cttataatcg aaatgtatat ctgcaataat tggtatattt acactagact
     9361 ttatctttgc tatattttta gcagctttaa tatcaggaac agcaactcta actatatcac
     9421 aaccagcttc ctctaatctc tttatttgag ctattgtagc atctgcatct cttgtatcag
     9481 tatttgtcat agattgtata cttataggat tttctcctcc tatttttaca gttcctacac
     9541 ttacttctct cgtctttctt ctctcgtaac tcatattcca cctctatata ttattttata
     9601 ttgaatctct ttaaatatta aaatattata acctacatgt atattataaa ttaaaaagct
     9661 atctagttaa ataagaataa atttcatata taaaaattaa tttattctta tttaaaacta
     9721 catagcctat tttcttaaaa tctctaaaaa ctaaatcatt gaaataatct tagtatatct
     9781 ttatatgtga taaaaagcat aaataacatt aaagctccaa aaccaacaac atttatcatg
     9841 ccttctttat ttgggtcgag ttttttacca cctctaacag cttctagtaa cagcataagt
     9901 attctccatc catcaagtgc tggtattggt aatagattta ctatacctaa atttaagctt
     9961 atgacagctg ctaaatacac tacatttatg atgcctgtac gagcagcatc tgataccata
    10021 ccaataacac ctactggtcc tgctacagca ttgccaatcc ctcctggtac acgtcctgta
    10081 atcatttgac ctagaaatgt tagcatctgt tttgtcatat ctacagtagt tttacatgca
    10141 tttactattg accccaaaaa gtctttttct ctttgtggtt gaatccctat ttcatacttt
    10201 ccattgttat ttttaggtgt tacatttaca actttatcac ttccatctct agttatacta
    10261 agttttaatt ctccaccaga agaagtattt attatatttg caacttcatc ccaagtctta
    10321 acagaattac cattaatttt atttatctta tctcctactt gaagccctac agcctgtgca
    10381 ggagtatctg gcattatctt tcctaattta gtcgttggag aacctatata caaaaatact
    10441 ggaactaaaa gtattgctgc taatattata ttgaatattg gtcctgctac aatagtacta
    10501 aatctttgta atatagactt gtttccaaaa gaatttggac ttatttgttc ttcatcttca
    10561 ccttccatgc tgacatatcc accaattgga agtgctctta tagaatactc tgtatctttt
    10621 tttacactat atatcttagg ccccatacct atagaaaact catttacttt gataccactt
    10681 ctttttgcaa atataaaatg tcctaactca tgtattaata caattatact aaatagtatt
    10741 aatgcggcta ttatagtcat aaattcacct tcctattttt ttatcagatt tgcaacataa
    10801 gctctactcc atctatctgt ttctaagatt tcttctaatg ttggttcact tatgctacta
    10861 tgaacttcta aagtcttctc tatgtaatat ggtatgtcat agaaaccaat tttatcctct
    10921 aaaaattcac ttactaaaac ttcatttgct gaatttaaaa cagaagaata agttcctccc
    10981 atttttaaac actcatatgc caattttaaa catggaaaag tttctaaatc tggttcttca
    11041 aaagttaaag tactaaattt tgaaaaattc attctttcaa agctactttc cattctatct
    11101 ggatatgtaa gagcatactg tattggtagt ctcatatctg gacatcctaa ttgagcaatt
    11161 attgagctat ctgtatactg caccattgaa tgaattatgc tttgtgggtg aactactaca
    11221 tctatatttt cttgttctac tccaaataac catctagctt ctatcacttc aagtccttta
    11281 ttcataagcg ttgaagaatc aatacttatt tttcttccca tactccaatt tggatgcttc
    11341 aaagcttcat ttttagttat atttgcaagt tctccctttt tctttcctct aaatggacca
    11401 ccagatgctg taagtattat cttttctata ttttttttat tttctccatt tagacattga
    11461 aatatagcac tatgttcact atcaacagga agaatattta cattgtattt ttctgcttct
    11521 ttcattacaa gttcaccagc agttaccaac gtttctttat tcgctaatgc tatgtctatt
    11581 cccttcttta tagcacaaag agttgggact aatcctatca tacctactac agcagttaaa
    11641 aggacatcca cttcatcaag tgatgatatt atttttaatc cttccattcc tgctaaaact
    11701 tctatttcta tatttttagg caacatttct tttaatttca atgctttatt ttcctcaaaa
    11761 actgttacat atttaggttt aaactctact atctgttcta gcaataattc aatattactg
    11821 ttagctgata ttgcaactat ctcaaactta tctctattat ctctaactac atccaaagtt
    11881 tgtttaccta tagagcctgt agaacctaaa attgatatct ttttcatata ataatccttt
    11941 ctaataataa atataccctg tcaaaacatt ataacactac taaaacaatt atttttacta
    12001 ttcatagaaa tttcatatat tattccacag attaaaatta atctattatt ttttgtattt
    12061 tgtggaatat tataaaatat tctagtatta ttaaatgccc ttttttattt ccacaacatc
    12121 ttctatttta cagtctagtg ctaaacatat cttctccatt gtatccatag aaacagattt
    12181 atcatttcct agtctgccta aaacagaaat acttatacct gatatattta caaattccat
    12241 tttctttatt tttttgtcaa ttaacaattt ccataatgga gcgtatgaaa tcatgttatc
    12301 acctcgcaaa atatattcta tcacatttta tctattttgg aaaattttca tctatttaga
    12361 taaataatta tttatctaaa tagatttcaa ttatagttta ttaaatataa ata
//
LOCUS       DAENCV010000110        12143 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAENCV010000110 DAENCV010000000
VERSION     DAENCV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.111"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            147..935
                     /locus_tag="KQD82_002970"
     CDS             147..935
                     /locus_tag="KQD82_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG4381428.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1406..5122
                     /gene="rpoB"
                     /locus_tag="KQD82_002971"
     CDS             1406..5122
                     /gene="rpoB"
                     /locus_tag="KQD82_002971"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG4381429.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5164..8649
                     /gene="rpoC"
                     /locus_tag="KQD82_002972"
     CDS             5164..8649
                     /gene="rpoC"
                     /locus_tag="KQD82_002972"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG4381430.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8938..9360
                     /gene="rpsL"
                     /locus_tag="KQD82_002973"
     CDS             8938..9360
                     /gene="rpsL"
                     /locus_tag="KQD82_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG4381431.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9484..9954
                     /gene="rpsG"
                     /locus_tag="KQD82_002974"
     CDS             9484..9954
                     /gene="rpsG"
                     /locus_tag="KQD82_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG4381432.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10003..12069
                     /gene="fusA"
                     /locus_tag="KQD82_002975"
     CDS             10003..12069
                     /gene="fusA"
                     /locus_tag="KQD82_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG4381433.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4704 a         1532 c         2495 g         3412 t
ORIGIN      
        1 attttagttt aaaaaaatac acgagtgttt tataaataaa atgtaataaa taattaatta
       61 acaaatataa attttaaaat aagttagata tttagaaaaa tcgaaatttg taggatttaa
      121 aggacatatt caaggaggag ttaataatgg aaaaattaca aggaaaaatt gcagtagtta
      181 ctgcagcaac aaaaggtatt ggattagcat cagcagagat attagcaaaa aatggagcaa
      241 ctgtgtactt agcagctcgt tcagaagaat tagctaatga agttataaat aaaataagtg
      301 cagaaggtgg ttgtgctaag tttgtttact ttaatgctcg tgaagaagag acctttacct
      361 caatgataga agaggtagtt aaaaaagaag gtaagataga tatacttgta aacaattttg
      421 gttcaacaaa cccttctctt gacaaagacc ttgtaactgg agatacagat aatttctttg
      481 acacagtaaa tactaattta aaaagtgtgt atttaccatg taaagcagca attcctcata
      541 tgataaagaa tggaaaagga agtatagtaa atatatcaag tataggttca gtattacctg
      601 atttatctag aatagcttac tgtgtatcaa aagcagcaat caactcatta actcaaaaca
      661 tagctacaca atatgctaaa gacaatgtta gatgtaatgc tgtacttcca gggcttattg
      721 caactaaagc agcattagac aatatgtctc cagagttcat aaaagaattt ttaaagcatg
      781 ttcctttaaa tcgtataggg gaaccagatg atatagcaaa agctgttcta ttctatgcta
      841 gtgatgactc atcatttata acaggggatt tacttgaagt tgcaggagga tttggtttac
      901 ctactccaca atttgcagat aatatattag gataattaaa ttactaatat gcaatgaaag
      961 cttatgcatt agttatagta gaaatgatat ttttataaag gccatagata taatttatat
     1021 tggaaaggtt tgaggttcaa aattccaagt taaatttatt tatggtcttt attttgtata
     1081 ttttaataaa ttgtatatat ttgtataaat gtaattattt acaatcattt taatatggaa
     1141 ttataattaa atagggggga tggtacttgt ataaaatttt ttatagaaaa ttagacctat
     1201 aaatgaaaaa tatggatttt atattggaaa attgtacata atacataata tatgcttaaa
     1261 ataatttaca aaaaatcaaa aataatgcat agtttacata taagataaaa aatatattga
     1321 aaaatacttg acattatgct aaaattatta gatgcgttag aatgaactat tgttttattt
     1381 ttaattattg agaggtgaaa agtgaatgcc acatcctgtc acgataggta aaagaactag
     1441 aatgagcttt tctaaaatta aggaaatagc tgatgtgcca aatcttatag aaattcaagt
     1501 agattcctat gagtggtttt taaaagaagg tttaaaagaa gtgtttgatg atatttcacc
     1561 aatagaagat tatactggta atcttatatt ggaatttgtc gactattctc tagatgataa
     1621 accaaagtac gatatagaag agtgtaaaga gagagatgct acatattgtg cacctctaaa
     1681 ggttaaggta agacttataa ataaagaaac aggtgagata aaagaacaag aagtgtttat
     1741 gggtgacttc cctttaatga cagaaagagg aactttcgtt ataaatggag cagaaagagt
     1801 tatagtaagt caattagtta gatctcctgg tgtttactat gcggaagaaa gagataaaac
     1861 aggtaagaga ttgatttcat ctactgtaat tcctaataga ggagcatggt tagaatatga
     1921 aacagattct aatgatgtaa tatctgtaag agttgataga acaagaaaac aaccagttac
     1981 agttttactt agagctttag gaataggaac agatgcagag ataattgacc ttttaggaga
     2041 agatgagaga ttatctgcta cattagaaaa agataatact aagacagtag aagaaggtct
     2101 tgtagagata tacaaaaaat taagaccagg tgaacctcct actgtagaaa gtgcatcatc
     2161 tttattaaat gctttattct ttgacccaaa aagatatgac ctagcaaaag ttggtagata
     2221 taaattcaat aagaaacttg ctttatgtta tagaataatg aataaaattt cagcagaaga
     2281 cataataaat ccagaaactg gagaagtatt tgtaaaagct ggagaaaaaa taagctatga
     2341 tttggctaaa gccatacaaa atgcaggtat aaatgtagta aatctactaa tggatgatga
     2401 taaaaaagtt agagttatag gaaataattt tgtggatatt aagtctcaca ttgattttga
     2461 catagatgat ttaaacataa aagaaaaagt acattatcca actttaaaag aaattttaga
     2521 tggatatagt gatgaagaag aaataaaaga ggctataaaa tcaagaatta aagaacttat
     2581 accaaaacat atattattag atgatataat agcttcaata agttatgagt tcaatatatt
     2641 ctataatata ggaaatattg atgatataga tcacctagga aacagaagaa taagatctgt
     2701 aggtgaattg ttacaaaatc aagttagaat tggtctttca agaatggaaa gagttataaa
     2761 agaaagaatg acggttcaag atatggaagc tataacgcct caagcgttag ttaacataag
     2821 accagtttca gctgcaataa aagaattttt tggtagttct cagttatctc agttcatgga
     2881 tcaaacaaat cctctatctg agttaacgca taagagaaga ttatcagccc ttgggcctgg
     2941 aggtctttca agagaaagag ctggattcga agtgcgtgac gttcaccatt cacactatgg
     3001 tagaatgtgt ccaatagaga ctccagaggg accaaatatc ggtcttataa actctctagg
     3061 aacttatgca aaaataaatg aatttggatt tatagaatca ccatatagaa aatttgataa
     3121 agaaacatca acagttactg atgaaataca ttatttaact gctgacgaag aagatttatt
     3181 cgtaagagct caggcaaatg agccattaac agaagatggt aaattcgtaa accatagagt
     3241 tgtttgtaga actgtaaatg gtgctgttga aatggttcct gaaagtagag tagattacat
     3301 ggatatatct cctaagcagg ttgtatctgt tgccactgct atgatacctt tcttagaaaa
     3361 tgatgatgcc aaccgtgccc ttatgggagc gaacatgcag cgtcaggcag tgcctctagt
     3421 tagaagagaa gcaccaatta taggaactgg tatagaatac agagcagcaa aagactctgg
     3481 agcagttgtt gttgctagaa attctggtat agcagaaaga gtaacagcag atgaaataat
     3541 tataaaaaga gaagatggaa acagagatag atataatcta cttaagttta aacgttcaaa
     3601 ctcaggtact tgtataaatc aaacacctat aataaataag ggtgaccaga taataaaagg
     3661 tgatgttata gcagatggtc cagcaactga tttaggagaa gttgcacttg gaagaaactg
     3721 tcttatagca ttcatgactt gggaaggtta taactacgag gatgctatat taataaatga
     3781 aagattagtt aaagaagata gattatcaac aattcatata gaagaatatg aatgtgaagc
     3841 tagagataca aaactagggc cagaggaaat aactagagat atacctaatg ttggagatag
     3901 tgcaattaag aacttagatg acagaggtat aataagaata ggtgcagaag tagactcagg
     3961 tgatatatta gttggtaaag taactccaaa aggagaaact gaacttactg ctgaagaaag
     4021 attacttcgt gcaatatttg gagaaaaggc tagagaagtt agagatactt cacttaaagt
     4081 gcctcatggt gaatctggta taatagttga tgtaaaagta tttactagag aaaatggaga
     4141 tgatttatct ccaggagtaa atgaattagt aagatgctat atagctaaaa agagaaaaat
     4201 aaaagttgga gataaaatgg ctggtcgtca tggtaataaa ggggttatat caagagtatt
     4261 acctgaagaa gatatgccat tcatggaaaa tggaacacca ctagacataa tacttaaccc
     4321 acaaggtata ccatcacgta tgaacattgg tcaggtttta gaagttcatt taggacttgc
     4381 tgctaagaca ttaggatggt atgtagcaac atcagtattc gatggtgcta atgagtatga
     4441 cataatggat gctcttgaag aagctgggta tcctagagat ggtaaattaa ctttatatga
     4501 tggtagaaca ggtgagtctt ttgacaacag aataactgtt ggatatatgt actacttgaa
     4561 actacatcac ttagtagatg aaaaactaca tgcaagaagt actggaccat actcattagt
     4621 tactcaacag ccactaggtg gtaaagcaca atttggtgga caaagatttg gagagatgga
     4681 ggtttgggct ctggaagcat atggagctgc tcacatactt caagaaatac ttactgttaa
     4741 gtctgatgac gttgtaggtc gtgttagaac atatgaagct atagttaaag gtgaaaatat
     4801 acctgaacca ggaatcccag aatcatttaa ggttcttata aaagaacttc aaagtttatg
     4861 cttagatgta aaagtattga cagatgaaga ccaagaaata gaagtaagag aatctgtgga
     4921 tgaagatgat acaataggtg aatttgagct agatgttgta aatcatatgg gagaagttga
     4981 agaaagtaat ataatagaag aaattgaaga tgattttgca gaaaatgcag aagatgagga
     5041 tatagagaat ttagaagaat tcactgaaga tgatttattt gaagaagaaa tagattttga
     5101 tagtgatgac tttgatatgt aaatttaatt aatgtaaatc tcaaatagaa gggagagaac
     5161 tccttgtttg aattaaacaa tttcgagtcg ataaaaatag cattggcttc tccagaaaaa
     5221 ataagacaat ggtctagggg agaagttaaa aagccagaaa ctataaatta ccgtacttta
     5281 aaaccagaaa aagatggtct tttctgtgaa agaatatttg gaccacaaaa agactgggag
     5341 tgccactgtg gtaaatatag aagagttaga tataaaggtg tagtttgtga tagatgtgga
     5401 gtagaagtaa ctaaatcaaa agtaagaaga gagagaatgg gacatataga gctagctgct
     5461 cctatgtctc acatctggta cttcaaaggt ataccaagta gaatgggact tttacttgat
     5521 atgtcaccaa gatcgttaga aaaaatatta tactttgcct catatgtggt agttgatcca
     5581 ggagagactg gattaaacga aaaacaattg cttacagaaa aagaatacag aactgctctt
     5641 gaaaagtatg gatatacttt tactgtagga atgggtgctg aagctgtaaa gacattacta
     5701 caaaatatag atttagagca acaaagtaaa gacctaagag cagagttaaa agatagtaca
     5761 gggcagaaga aagttagaac aataagaaga ttagaagttg tagaggcatt taaaaagtct
     5821 ggaaataaac cagaatggat gattttagat gcaataccag taataccacc agatttaaga
     5881 ccaatggtac aacttgatgg tggaagattt gcgacttcag acctaaatga tttatataga
     5941 agagttataa atagaaacaa tagacttaaa agattattag agcttggagc tccagatata
     6001 attgtaagaa atgaaaaaag aatgcttcaa gaagctgttg atgcattgat agataatggt
     6061 agaagaggta gacctgtaac aggacctgga aatagaccac ttaaatcttt atcagatatg
     6121 ttaaaaggta agcaaggtcg tttccgtcaa aacttacttg gtaagcgtgt tgactactca
     6181 ggacgttctg ttatagttgt tggaccagaa cttaaatttt atcaatgtgg acttccaaag
     6241 aagatggcat tagaattatt caagccattt gttatggata aattagttaa agaaggttat
     6301 gcacataata taaaaagtgc gaaatctata gtagaaaaag ttaagccaga agtttgggat
     6361 gttttagaag atgttataaa aagtcatcca gttcttctta accgtgcacc gactctacat
     6421 agattaggta tacaagcatt tgaaccaatc ttagttgaag gtaaggctat aaaactacat
     6481 cctcttgtat gtacagctta taatgcagac tttgatggtg accaaatggc agttcatgta
     6541 cctttatcag tagaagcaca agcagaagca agattcttaa tgctttctgt aaataatata
     6601 cttgctccta aagatggttc acctataact actccatctc aggatatggt tttaggttgc
     6661 tattatctaa caatagaagc ccaagatgga gctaaaggaa caggtatggt atttaaagac
     6721 tttaatgaac tattacttgc ttattacaat aaatcagttc atctacatgc attagtgaaa
     6781 cttaaggtaa cacttgaaga tggaagaagt tcattggttg aaagtactgt tggtagattt
     6841 atatttaatg aaaatatacc tcaagactta ggttttgtag atagaaaaga aaatccattt
     6901 gcacttgaag ttgatttctt agcagataaa aaatctcttg gtaaaataat agataaatgc
     6961 tttagaaaac acggaaatac agaaacagct gaattactag attatataaa agctctagga
     7021 tttaaatatt ctacattagg tggcataaca gttgctgttg atgatatgag tgtcccagaa
     7081 gaaaagaaag tatttatagc tgaggcagaa gctaaagttg ataagtatga aaaagcatac
     7141 agaagaggtc taatctctga tgaagaaaga tatgaaaaag ttatagagac atggacagaa
     7201 acaactgata aagttactga tgctcttatg ggtggactag atagattaaa taatatatat
     7261 ataatggcac attcaggagc cagaggttct aaaaaccaaa ttagacagct ggcaggtatg
     7321 cgtggtctta tggcaaatgc atctggtaaa acagttgaga taccagttaa atctaatttc
     7381 cgtgaaggtt tatcagtact agaatacttt acatcttcac atggtgccag aaaaggtctt
     7441 gccgatacag ctatacgtac agctgaatct ggatatttaa caagaagact tgttgatgta
     7501 agtcaagatg ttattgtaag ggaaatagat tgtggaacag aagatactac agagatttat
     7561 gctataaaag aaggaaatga agttatagaa gagatatatg atagaattgt aggaagatat
     7621 actatagacc ctatattaaa tcctgaaact ggagaagtta tagttgaggc tgattcaatg
     7681 atacaagaag atgaagcaga aactatagta gctttaggaa ttgaaaaaat taggataaga
     7741 acagttctta actgtaaaac taatcatgga gtttgttcta agtgttatgg tagaaacttg
     7801 gcaacaggaa aagaagttaa tataggtgaa gcagttggta taatagcagc tcaatctatc
     7861 ggtgagccgg gtactcagct tacaatgcgt acattccata ctggaggagt tgcaggagct
     7921 gatataactc aaggtctacc aagggttgaa gaattatttg aagctagaaa accaaaagga
     7981 ttagctgtaa taactgaagt atctggtaga gttgaaattg atgaaacagg aaagagaaaa
     8041 gaagtaaatg taataccaga agaaggcgag actcaaacat atgtaattcc atatggttct
     8101 agattaaaag ttaagcaagg tcaaatgcta gaggctggag accctctaac acaaggattt
     8161 ataaatcctc atgatatagt aagagtaaat ggtgttaagg gagttcaaga atatatagtt
     8221 aaagaagttc aaagagtgta tagacttcaa ggggttgatg ttaatgataa gcatatagaa
     8281 gttatagtaa gacaaatgct atctaaagta aaagttgaag acccaggaga tacagattta
     8341 ttgccaggtg gatatgaaga tgtattaaca ttcaatgaat gtaataaaga tgctatagat
     8401 aaaggtctaa gaccagcagt tgctaaaaga gttttacttg gtataactaa agcatctctt
     8461 gcaactgatt cattcttatc agcagcttct ttccaagaaa caacaagagt attaacagag
     8521 gcagcaataa aaggtaaaga agaccactta ataggactta aagaaaatgt tatattaggt
     8581 aagttaatac cagcaggaac aggaatgaag aaatatagaa atatagctgt tgaaaaaatt
     8641 gaagattaat agcatataga gtaaagaaat cttgacaacc attatttgtg gtgttaaaat
     8701 agtaaagtgt gttgtttaaa agtacactaa gggcttgaac aagttcttag tgacttttag
     8761 atggcaataa atgagaacta aaaagcatca agctagaaaa gtgctaatat cacatattta
     8821 tgtgttatag acattaaatt gtaagtaacg aatgttacaa tcagtcagta tgaatattca
     8881 tattgatgta gttggcggtc actgataaga tcgaaaattt ggaaggaggt gcagatgatg
     8941 ccaacaatta accaattagt tcgtaaaagt agaaaagcgc tagagaagaa atctactgct
     9001 ccagcattac aaaaaggtta caactcttta aataagaaag taactgacgc tagtgcccca
     9061 caaaaaagag gggtttgtac ttcagtaaaa acagttactc ctaagaagcc taactcagct
     9121 ttaagaaaag ttgccagagt tagattaact aatggtatag aagtttctgc ttatatacca
     9181 ggagaaggac acaacttaca agagcatagt gttgttctta taagaggagg aagagtaaaa
     9241 gaccttccag gggttagata ccatatacta agaggtactc tagatacagc aggtgttgat
     9301 aagagaagac aatcaagatc taaatacggt gcgaagagac ctaaagaagc taaaaagtaa
     9361 aaacaattaa tgattgatta tagttcggtg ctttaaatat atatatattt acggcatcac
     9421 ggcattaaat gatttatgtc gagtacctat gatttaagtt aaataattaa ggagggaagc
     9481 gaaatgccaa gaaaaggtaa tattccaaaa agagaagttt taccagatcc tatgtatggg
     9541 agtaaggtag ttactaaatt aataaataat ctaatggtag acggaaaaaa aggaaagtct
     9601 caaagaatag tttatgatgc tttcgcaatt gttgctgaaa agacaggaga agaagcttta
     9661 gaagtattta ataaagcaat ggataatata atgcctgttt tagaagtaaa agcaagaaga
     9721 gttggtgggg caaactacca agtgccaatc gaagttagac ctgaaagaag acaaacatta
     9781 ggtttaagat ggctagtaaa atatactaga gctcgtggag aaaaaggaat ggttgaaaag
     9841 ctagctaaag agataatgga cgcagctaac aacactggag cttcagttaa gaaaaaagaa
     9901 gatactcata aaatggcaga agctaataaa gcatttgctc attacagatg gtaggatata
     9961 tagatatctt ataaaattga ggatatcata aggaggataa ccatggctag aaagtttcct
    10021 ttggaaagaa ctaggaatat aggaatcatg gctcatatag atgcaggaaa aactactact
    10081 acagaaagaa tcctgtttta tactgggcaa actcataaaa taggagaaac tcatgaagga
    10141 gcttctcaaa tggactggat ggagcaagaa aaggaaagag gtataacaat aacttctgcc
    10201 gctactactg cttcatggaa agaccataga ataaacataa tagatactcc tggacacgtg
    10261 gacttcacag ttgaggttga aagatctcta agagttcttg acggttcagt tgctgtattc
    10321 tgtgcaaaag gtggggttga gcctcaatca gaaaatgtat ggagacaagc tgaaacttat
    10381 ggagtaccta gaatagcttt tgtaaataaa atggatatat taggtgcaga tttctacaat
    10441 gttgtaagca tgatgaaatc tagattaaat tctaatgcag ttcctatgca attaccaata
    10501 ggtaaagaag attcatttat aggtataata gacttattaa aaatggatgc tgttatatac
    10561 aaagacgacc taggggttga aatggaagaa acagatatac cagaagatat gaaagaatta
    10621 gctgctgagt ggagagaaaa attagttgaa tcagtagctg aaactgatga agaattaatg
    10681 atgaaatacc ttgaaggtga agaacttact atagatgaat taaaagttgc tataagaaaa
    10741 gctactatag cttgtgaaat gaacccagtt ttctgtggta cagcttatag aaacaaaggt
    10801 gttcagttag taatagatgc agttttagat tacttaccag cgccaactga tataccagct
    10861 ataaaaggta tattagcaga tggagaagaa gctgaaagac attcttctga tgaagagcca
    10921 ttctctgcat tagcattcaa aataatgact gacccattcg ttggaaaact agcgttcttc
    10981 agagtttatt ctggaacatt agaatcaggt tcttatgttt taaatgcaac taagaataag
    11041 agagaaagaa ttggtcgtat attacaaatg catgctaata caagagaaga aataactaaa
    11101 gtgtatgctg gagatatagc agcagcagta ggattaaaag atactactac tggagacaca
    11161 ttatgtgacc ctgctaatcc aataatactt gaatctatgg aattccctga gccagttata
    11221 tctgttgcta tagaaccaag ttctaaagca gctcaagaaa aaatgggtat agctcttcaa
    11281 aaattagctg aagaagaccc aactttcaca gttaagactg accaagaaac aggacaaact
    11341 ataatatctg gtatgggtga attacactta gagataatag tagatagatt attaagagaa
    11401 tttaaagtag aagctaaagt tggtgcacca caagttgcat atagagagac tataactcaa
    11461 ccagttgatg ttgaatacaa gtactctaag caatcaggtg gtagaggaca atacggtcac
    11521 gttaagatta gagttgctcc tcaagagcca ggtgaaggat acaaattcac aaacaaaact
    11581 gttggtggat cagtaccaaa agaatatgtt ggtccagttg acatgggtat acaaggagct
    11641 atgcaatctg gtatagttgc tggatatcca gttgtagacg ttgctgttga gttatatgat
    11701 ggttcttacc atgaggttga ctcttctgag atggcgttta agatggctgg ttcaatggca
    11761 ttcaaagatg ctatgaagaa aggtaatgca gttcttttag agccttactt caaagtagaa
    11821 gttgttactc cagaagatta catgggagat gttatgggtg acttaaactc aagaagagga
    11881 ttaatccaag gtatggaagc tagaagtggt gcacaagtta taaatgcatt cgttccactt
    11941 tcagaaatgt ttggatactc aacagaccta agatcttcaa ctcaaggtcg tgcaacttac
    12001 acaatgatat tcgatcacta tgagcaagtt ccagcttcag ttgctaagaa aatagctgaa
    12061 ggaaaataat ttaaaaataa ttaataatat aaatataaaa aataatcagc tagatgagag
    12121 atatctctca tctagcacaa gat
//
LOCUS       DAENCV010000111        11880 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAENCV010000111 DAENCV010000000
VERSION     DAENCV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.112"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            371..1297
                     /locus_tag="KQD82_002976"
     CDS             371..1297
                     /locus_tag="KQD82_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG4381434.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(1657..3309)
                     /locus_tag="KQD82_002977"
     CDS             complement(1657..3309)
                     /locus_tag="KQD82_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG4381435.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            3908..5029
                     /locus_tag="KQD82_002978"
     CDS             3908..5029
                     /locus_tag="KQD82_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG4381436.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            5065..5478
                     /locus_tag="KQD82_002979"
     CDS             5065..5478
                     /locus_tag="KQD82_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381437.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            5471..6010
                     /locus_tag="KQD82_002980"
     CDS             5471..6010
                     /locus_tag="KQD82_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4381438.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(6210..6695)
                     /locus_tag="KQD82_002981"
     CDS             complement(6210..6695)
                     /locus_tag="KQD82_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381439.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            7600..7977
                     /locus_tag="KQD82_002982"
     CDS             7600..7977
                     /locus_tag="KQD82_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG4381440.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            8373..10178
                     /locus_tag="KQD82_002983"
     CDS             8373..10178
                     /locus_tag="KQD82_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4381441.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            10255..10794
                     /locus_tag="KQD82_002984"
     CDS             10255..10794
                     /locus_tag="KQD82_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG4381442.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            11408..>11880
                     /locus_tag="KQD82_002985"
     CDS             11408..>11880
                     /locus_tag="KQD82_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HBG4381443.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNK"
BASE COUNT         4574 a         1315 c         1909 g         4082 t
ORIGIN      
        1 aatacacaac ttatgatatg gacgaattat tatgcaaata agctgtaatt aaataaattg
       61 caatataaac cagttgaaac ataagttagg gaaatattat caaagttatt atttagcata
      121 agagtataga gggaataaaa taagattaaa aaattacata ctaaatttaa taaaattttt
      181 atattaaaaa taatattaag tttattgttt aaataaaaaa gttaaatcta cactattaac
      241 ttattatact taaaacttta aaatgaaaat atcaaagaat actttttaat aaaaataatc
      301 atttaataag ttatttaaaa atctaaaaac tttacaacaa attatctatt taaattggag
      361 gagtgggaat atgagtaata gtttttatca attaaattca gcagtattat attttaatga
      421 aaaaagagat gtaaatagaa taaggtttaa tcactatgta atgagaaaag aaaatataca
      481 aagattaaaa ggaactctgc caagaggaca atttatgatg acagtaagga aagctgcatc
      541 tgaccttgat gtatctatat ctacaataag tagacttgta aatgaattta tagatttagg
      601 tatattgagg ttaataagta ggggagtaaa gggaaattgt tgttctgtat atagttatgt
      661 ttgttctgaa agtagtaatg cagaaattgt aacaaagaat ggtgatattt tcaaaaatat
      721 agagaaatac attgatgcaa ctattaacaa taaccaaaaa acaatgaaat ataaacatag
      781 agatgagttg tttaagaaca gtaataccaa catatacagc cgtataaatc aagagagtat
      841 agacaaagat tatgatttga taattcatac tgggtatgaa acagaaaatg agacaaagaa
      901 aaaagaatta ttaaaaaata atttaaataa aaagaattta aataaaaata cctttgaaaa
      961 ttcggatgaa gatttgactg aagattctac ttataaaaat ccagaaagca tacatgaaat
     1021 tattataaga aaattaaata gtgaatcagg aaaaaatttt aaagctgatt tgagtataac
     1081 taaggcactt atagataaaa gattaaaaga agggtatagt ttagaagatt tttacaaggt
     1141 tatagaggca aaggtaagaa gatggagata tacagatatg gaaatatata tagaaccaac
     1201 aattctgttt gggtatgaat ttgagtcgta tttaaatgag gagagttctt tatctgcttg
     1261 taattatggt gatatgtgtt atgaagatat gtattgagat atttattaag atattagttg
     1321 ctttttagtt gtttaaaata tgtttatgta taaatagtgt attaaaatgg agttgtctca
     1381 aaatagagag aagcctcttc ttaattgttt aaatgatgaa aatatatttg atttaataca
     1441 aaggagagtg ctttatgagt tagaagattt attttgagac actcactttt tttaatatac
     1501 tggagaaata taacccattt tacaagagtg cataggtata tataatggca taatgtggat
     1561 ttaaattaaa aaaaataaaa taaagccata aatattgaaa ttttcaatat ttatggcttt
     1621 attgcttgta ataattaata gaatatatat tttttattaa tcatctattt caaattctaa
     1681 tattttacca gtaattgcat taattgttat ctcatattca gtttttcctt ttattatttc
     1741 accttcatat tctttgtcat cttcatcata cttaaatttt actattttcc caccaggcac
     1801 tttatctagc attattgatt tagctttttc agcactaata atagaactgt tgtcattagt
     1861 attagagtta ttcttatcat tatcatcaaa gtcatcatca gaatcattgt ccatgtcaat
     1921 atctacaatt cttccatcaa atgcacttat ctctaattca tatgttttct tattttttat
     1981 tactttagcc tcatattcgt tatcttcagc atcatattta caatagatta aattaccatt
     2041 aggaatagca tctaatgcta tagacttagc tttatccaat gatatatttt gtgtacctaa
     2101 attattatca tttaagtcat catctttttc aatttctatt attttaccag agtaggcatc
     2161 aatttctatc tcatatagga tattatcctt tataactttg cagtcatatt cattatcatc
     2221 ttcatcataa taacaaacta aaatattgct tcctggaaga gcttgtttta atatttcttt
     2281 agcctgtatt aaagatattt ttttagtttt atttcttatg ttcacatttt ttagttttaa
     2341 aatatcttca aatgcttttt gattatatcc tcctcctact tgtgttattt catctatatc
     2401 tttattttta ataaattctt tttgactatt tacaaggcta ccattagata tcattatagg
     2461 agaattttct ttagcagcta gtactcctat tgataatgaa tctactaatg cattttcttg
     2521 gttaatacca tctttagcta caaatacatt atttattttt ttatctttat aaaatttatc
     2581 aataattttt gcattagtat tgtttctatc tgaaccactg attctttctc tgtttggata
     2641 tttctttact gtattgtctg ataaaatctt atttcctcca actatgtaag atttttttat
     2701 agacctattg tttaagaaat ttatactact atcattgctg ttttcatctg atactaatat
     2761 tataggcata ttatgtattg ctgctggaga agctatacta actgcatcag atagtccctt
     2821 tagtccattt acaactgcaa cattttctac cttttttatc ttatctattt ctttaagtac
     2881 atttgtagac atttcatata tattgtttcc tactatttta tttattttta tgttaagtga
     2941 ttttatttca ttttctactt tggaagaaat gctatttcca ctatctacaa tgtacacatt
     3001 atctacacca agccttttaa gctcagcctt agttgaattt tcaatactgt tgttgtttac
     3061 taaaagtata ggtgcattct ttagcttagc aaatggattt gcacaaagtg cactttgaat
     3121 agaattacca ttaactataa tggctgtttt tgattgaacc catcccaatt tacttacttc
     3181 tactgctgtt tcatatcttg ttttacctat taatctttct tgctttgtag tattgtctgc
     3241 aaaaattgct gaagaactag aaaaaaacat catacctgat attaatatag ctaaattttt
     3301 cttattcata tttttacctc acaatcttta aaaatttgtt atttagcata cttaataaaa
     3361 tataatctta aaatcaaatt gatgctgagt ttgaagttac aaaatcaaca aataagattt
     3421 ctttaataca aaattttcct ttttatattt aacattgtat cacaaatata aaaaggaaaa
     3481 aactttttaa aaagggtata cactattcat tttttatctt ataaataagt ctttaccact
     3541 gttttatata ttttaaaata gacacaagac ctatttctaa atcttttata aacattttag
     3601 gtgaaatttc tttttaaaca tatctaaaaa gtatttgtag taatattaat ggactgaatt
     3661 tcttaaagta tatatttctt tacatgttgg agtttgtgaa gtttgagaat tggagggtga
     3721 aagaaacatg taatggatta atattaaaga actgtatttt tataatattt gtcttaaaaa
     3781 taaaaaaatc tattgacttg aagttaactc caagtagtaa agtataatta tacaagaatt
     3841 atatacttgt aagaatagtt aaagtagaga gaaaaaacaa acaagtttta aagggggaag
     3901 taaaacaatg gaggcaaaaa aattaggatt tggattaatg cgtttaccaa taaaagatga
     3961 aaatgatgtt acaactattg atatggaaca tttaaataaa atggtagaca cttttttaga
     4021 aaggggattt acatattttg atacagccta tgtgtatcac atgggaaaaa gtgagatagc
     4081 acttagagaa tctctagtaa aaagacacaa aagagagagt tttactatag ctacaaaact
     4141 tccattaatg gctttaaaga aaaaagaaga gcaagaaacc atttttaatg agcaattaga
     4201 aaaatgtggt gtagactatt ttgactacta tcttttacac aatataggcg tgtcacatta
     4261 tgaagtagct aaaaaatttg atagctttaa gtttattgaa gaaaagaaaa aagaaggaaa
     4321 aataaagaat attggttttt cttttcatga tagtgcagaa ttattggata aagtcttgag
     4381 tgagcatcca gaagtagact tcgtgcaatt acagattaat tatttagatt gggataatga
     4441 aagcatacaa tctagaaaat gctacgaggt ggcagagaag cataataagc ctgtaattgt
     4501 tatggagcca gtaaaaggag gtacacttgc aaagatacca gaaaaagcag agaaattatt
     4561 gaatgactac aatccagata tgtcaatttc atcttgggca atacgttttg cagcaagtaa
     4621 agataatgtt atgatggtct taagtgggat gtcaaatatg gaacaattat tagacaatac
     4681 aggatatatg caaaatttca aaccattcgt acaagaggaa tataatatta tagatgaagc
     4741 agtagagatt atcaacgaat caattttagt tccttgtact gcctgtcagt attgtgtaga
     4801 aggctgtcca aaaaatatcg ctattccaaa ctattttgcg ttgtacaatg cagaaaaaca
     4861 agctcttaat actggatttt caacacagat ggtctactat aataattata ctaaaactta
     4921 tggaaaggcc tcagattgta ttgaatgtaa acaatgtgaa gaaagctgtc ctcaacacat
     4981 taaaattata gatgctttaa agaatgtagc agaaaccttt gagaaataat tcaatctata
     5041 caatatcaag aggagaagat atgtatgacg atttcagaag ttagtaaaaa atacgagctt
     5101 tcagcggata ctttacgata ttatgaacga attggtttaa taccacctgt aaatagaaat
     5161 aaaagtggaa ttaggtcttt tacagaagaa gattgtgagt gggttaattt cataaagtgt
     5221 atgagaggtg caggtctttc tattgagaca ttaattgagt atgtagctat gtttcaacaa
     5281 gggagcagta ctatcaaagc tagaaaagaa cttttaatag agcagagaaa ccaactagca
     5341 aaaagaatag aagaaatgca aaaaactttg gaacgattaa attttaagat tgatagatat
     5401 gaagaaggta ttattgagaa agaaaaagta ttaaagagca gtagaaataa gaaaatggag
     5461 gcagtatctt atgagtaaaa atgtattaat agtatcagct agtcctagaa aaggtggaaa
     5521 ttctgattta ttgtgtgacc agtttataca aggggtatta gaatctaaaa atcaagtgga
     5581 gaagatattt ttaaaagata aagatattaa ctattgtact ggatgtggtg cttgttatga
     5641 aaaagaagca aattgttcac agacagatga catgggagaa attcttgaaa aaatgattga
     5701 ggcagatgtg attgttttag cgactccagt ttatttttat actatgaatg cgcaaatgaa
     5761 aactatgatt gatagaactt gctctagata tactgaaatt tgtgataaag atttctattt
     5821 tatcgttaca gcagcagatg aaaatgtggc tgatatggaa agaacaatag aaggatttag
     5881 aggatttaca tcatgtctaa atggttctaa ggaaaaaggt atagtatatg gtgtaggtgc
     5941 ttggaatgta ggtgatatta agggaagtaa ggcaatgaaa caggcatatg aaatgggtaa
     6001 gttggcgtag tatgtttgta taaaattgta taaaattgaa tatattctta tcatttcaac
     6061 aataaaaaga ggcttatctg tattttgaga cagcctcttt gtgctatatc atttattttt
     6121 attctatatc cttattatac cctttatttt tttatgccta tttaagtatc tgttgttact
     6181 catatatttt aataattacc cttaaaaaac tatacagatt cctgcataat attattttct
     6241 ttagtaatta atttttctag gtttttccaa tccatttctt ccatatttgc aaaataatga
     6301 ccacttccta tgcaagtcag gtaagacttt ccatctaatt caaacgaaga acctccaata
     6361 cactttgaat tacatttctt acaagtacga gttttagtta tactatcttt aatagtttta
     6421 gagcattcct ccacagcatc tttatatgta tttagatgaa aaagatttgc tttaactctc
     6481 catttttttt cattacattc aatagtaaat aagctacgtt ttggtttctt agttgaatat
     6541 actattttgt aagaacgagc acctgttttt ggttcaattt tacaatctaa tgatttacca
     6601 agttcatgta aatgacatgc aatatcataa tctttaccac ttagtacttc taacatttct
     6661 tctgttgttt ctgctgttaa ttcacttttg ctcatattaa aaaaatcccc ctatataaat
     6721 aataatttca tatgaaaata attaatataa gtatattaaa taaaactatt aaaatcaata
     6781 aaattactgc aatcttacat tattgggcat agaatgagag ctaatgattt attagaaata
     6841 agacttaata gtatgaaaat ttacttagac gtggtacaat tctataaaag atttaaaact
     6901 aggaaaggag aaagttgttg agatacggcc aatataacaa tttatttata agttgtaaat
     6961 ttattatgag attaattaga ttgttaagta atactatcaa aaggtagttt tttggatttt
     7021 cagcaacagt ggtagatatt gataattttt tggttattat tatatttagc tgttatattt
     7081 tcaataggct ctatgataac tagggtaaat aaggtatggg taaaggtttt attatttata
     7141 atagtgggat tgtcaactat agttctttca tatgtaataa cttgattatg caaaattcta
     7201 tgaagcagga accctgtgac tttagtcatg ggaggttcag aatttaaggt taaaaaataa
     7261 ctttaatctg taaattctca aaacaagaat tctcatgttg gtaaatttct agatatgaga
     7321 aaaaaattga catcataaaa aagtaatttt agagcgtttt ttgacgaaaa aattcagaag
     7381 taactatttt gaatgacttc acaaaatttt atatataaat aaagtttggc acgttaattg
     7441 ctttaataaa agttaacaag acacaaataa acataatcat ctaagtattg attatttgaa
     7501 gttttagatt tgaaatttaa tctaaaggtt cgtattaata ttggtgttta ggaaatttta
     7561 ttttcctgta aatataactt aataatagga ggtgaagtca tgtcaaaagt agtaatagct
     7621 acagagaaag ctccaggagc aattggacca tattctcaag caattcaagt tggaaacata
     7681 gtttctacat caggtcaact tccaatagat atgaagactg gcgagttagt atctgaccca
     7741 aaagaagcaa caaaagcttc tttagataat gtaaaagcaa tattagaaca agctggtaca
     7801 agtatggata aaatatttaa gactacagta ttcgtaaaag atttaaagga ttttaatgat
     7861 attaatgaag tatatgctac atattttaaa gaaaatccac cagcaaggtc atgcgtacaa
     7921 gttgctcaat taccaaaaga tgcagtttta gaaattgaag caatggcagt attataagta
     7981 ttataacaat aaaaccaata acaaaactaa taaaaattaa aaactaataa aaaaagtcta
     8041 caattaaaaa agtttatatt aaaaaagtct atataaaaat ctatctaaat atttaactat
     8101 tgagcttttc tcatataatg tatattttta tgtcaaaaat atatttgtta tatgaggaaa
     8161 gctttttttt attattgtat tgtgatttat tttattgtct gcatattata attaaggtta
     8221 ctatataaaa caaacttttt ataaattaat attgtctgtt ttaacgcaaa tatataaata
     8281 agtaatgttt tatatatatt ttaaagagtg actatatgtt aaaatatagc taaactgata
     8341 atttggtaaa ttaaattcta atgggggaaa ctatgttaag taaattaaaa gaatttcaac
     8401 aagaaatgat aaaatacact gaaacagtag ctagtgtgtt agatgtagat attgaaatag
     8461 ttgacgatag attaataaga attagtggta caggtctgta taaatcaaag ataaatgaaa
     8521 gcgttgttac agaaggattt atttatgaca atgttataca gacaggtcag gaattagtcg
     8581 tattggatat atgtgataat cagctttgta tagaatgttc ccattacatg aaatgcttaa
     8641 ataaagtaat aatagcagtt ccaataaagt ataataatcg aactatagga gtaattggtg
     8701 ctattagtac agataaaact aaaaaagtag aaataagtgc aaaaatagat aattatttaa
     8761 aatttgttaa tcatatatgc gatttaattt ctatgaaaat agaagaacat gaagcgtcaa
     8821 aaaatagcag tagaaaaatg gatatgatga tagaaatcat tgaaaatgtt gaaaaaggcg
     8881 ttataatact agacataaat agcaaaatat cttatataaa caatatagct ctaaaaaaat
     8941 tagatataga taaaaatata atagaaaata tagtaaatat agtatctgtt gaatcttcct
     9001 caaatggaca tgaattacta gaaattgata ttgataacaa aatttataat ataaatgcca
     9061 aaataattcc ggtatatcca tatataaatc aatacgacaa aataataata tttgacaaga
     9121 catatatcaa tcataaagga catggaaaag tgaatagtgg ctggggaaat agtgatatag
     9181 agtctataat tggaaattca gaagcaatgt taaaagtaaa agagagaact aagaaattag
     9241 caaagtcaaa ttcaactgta cttataactg gggaaagtgg tacgggtaaa gagctaattg
     9301 ctagggctat acatgctgaa ggtagcagat ggaacaagcc atttattgcg ataaactgtg
     9361 ctgctattcc agaaaatctc cttgaaagtg agctatttgg ctatataaaa ggtgcattta
     9421 gtggagctag tagtggaggg aaagtaggta aatttgaact tgcaaatgaa ggtgttatat
     9481 ttcttgatga aataggagat ttgtcaatgc ctttacaagc aaagttactt agagttttac
     9541 aagagagaaa atttgcgaga attgggtcaa ataaacttat agatttagat ataagagtga
     9601 ttgctgctac aaataaaaac ttattgaaat tagtaaatga aggtaagttt agggatgatt
     9661 tatactatag attgaatgta attcctataa atttgccacc tcttagggaa agaaaggatg
     9721 atattgaggc aataatgatg aagtttgctt ctaaatattc tttagaatta ggtattcagt
     9781 taaataaaat agaagaaaat gtaatgaata tgttgattaa ctataattgg ccgggaaata
     9841 taagggaact ggaaaatgca gttgaatata tgatgaattt ggttggagat gatggaataa
     9901 tatataaaga tatgcttcca ttagatattt taaactatta taatattaat ggaaatattt
     9961 ataaaaataa ggatgtaaat ataatatttg aagatgatat agttggaggg atagtagaga
    10021 atcaagaaag gatactttca attaaagaat tagagttgac ttacataaac aagttgttga
    10081 ataagtatgg aagagataca aagacgaaga agaagatagc aaaagactta ggcataggtt
    10141 tggctacact atatagaaaa ttggaagagg aacagtagta atataatatt aaaatgatag
    10201 gtatttgtgt gtttttatag ataggtattt ttactttatg gtggaggtgt tattatgcaa
    10261 tatgcgatag agctatatta tgataaaaaa acggaaaagc aactttttga tttatctaaa
    10321 aaaatcgctg atgaaaaaat tagtacgaag tacttagaat ggaaaacaag acctcatctt
    10381 actttggctt gtttcaatga tgtggatgag gcttgttgca tagacaagct aaaggggttt
    10441 gcacaaaacc ataaggtaat acctgcttac attggttcag taggcatgtt taatgatact
    10501 aaaacggtgt ttgtatcacc tataatgaat agcagtatgt atcagtttca gagagaatta
    10561 tatgaggtac tacaagattt tgattcaaat ggttgggaat ggtaccaccc caatcgttgg
    10621 gtaccccatt gcacaatagc acttactgga gatgatgaaa gtgaggcttt ttttcgggca
    10681 agtgatgtat tactgcgtga gttcaaaaaa ataagtggtg agtttgtctc aataggattg
    10741 gttaaaatta catttcctgt ggaagaaatt tttacagtga atatgaatag gtaactttaa
    10801 ctctgttgtg gatgttatga atattttgta tagtgcattt catcagataa atgcgaagtt
    10861 gcgaatttcg acagctcaat agtcgcaagt agggcacaat ggtacacata ttgcactttt
    10921 gcttattggg gggacgccca tgtaaacttc tgtaagaagc tatgaaaaga tttaagccat
    10981 tttggaggct ttgggtaata aagaatataa agtatataaa gtatataaat ttatagagtt
    11041 tatgcgtaag aaggttatag ccatatattt atagaaaata aaagtagtaa tatatctgac
    11101 aaaacaatag acatataaaa aataagagca taataaagga agaattaaga caaataaacg
    11161 atttctcgaa ttgataattc tcgtattgat aaaaatttcc caatatgaga attttttctt
    11221 ttataaatct tatttttcac actcaaaatt tataaacttt aaagaaattt tataaaagta
    11281 tgtaattcca acataaaaaa attgttttta gaataaataa tattggcacg gtaattgctt
    11341 tgctataagg cacaaagata aaaaattaaa atgacaccga aaaatttgaa aggggaatat
    11401 aaaataaatg agagaaataa aatggaaatt aaataactta cctaaatcag aggaaaaaga
    11461 aaaaggaatt gaatttttaa aagatgaaga gattgcaaag gctaaagctt ttcatgagag
    11521 ctttccacaa tatgaaaaga ctcctttagt taaattagat aacttagcaa aattactagg
    11581 cgtaagcgga atatatgtta aagatgaatc ttatagattt ggcttaaacg catttaaagt
    11641 tttaggtggt tcttactcta tgggtagata cttagctcaa agattaaata cagatatatc
    11701 tgagttaggg tatgataaat taacttcaaa agaaataaaa gaaaaattag gagaaataac
    11761 tttctttaca gctacagatg gaaatcatgg tagaggagtt gcttggacag ctaataaatt
    11821 aggtcaaaaa tctgttgtat taatgccaaa aggttcttct gaatttagat taaataaaat
//
LOCUS       DAENCV010000112        11709 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAENCV010000112 DAENCV010000000
VERSION     DAENCV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.113"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            184..1647
                     /locus_tag="KQD82_002986"
     CDS             184..1647
                     /locus_tag="KQD82_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG4381444.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKLFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKKIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1703..2839
                     /locus_tag="KQD82_002987"
     CDS             1703..2839
                     /locus_tag="KQD82_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4381445.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3075..4190
                     /locus_tag="KQD82_002988"
     CDS             3075..4190
                     /locus_tag="KQD82_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG4381446.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLFCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4342..5211
                     /locus_tag="KQD82_002989"
     CDS             4342..5211
                     /locus_tag="KQD82_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4381447.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGNLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            5587..6132
                     /locus_tag="KQD82_002990"
     CDS             5587..6132
                     /locus_tag="KQD82_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG4381448.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            6145..7158
                     /locus_tag="KQD82_002991"
     CDS             6145..7158
                     /locus_tag="KQD82_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG4381449.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            7380..9353
                     /locus_tag="KQD82_002992"
     CDS             7380..9353
                     /locus_tag="KQD82_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG4381450.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEICVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            9422..9781
                     /locus_tag="KQD82_002993"
     CDS             9422..9781
                     /locus_tag="KQD82_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381451.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            9816..10172
                     /locus_tag="KQD82_002994"
     CDS             9816..10172
                     /locus_tag="KQD82_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG4381452.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            10209..11501
                     /locus_tag="KQD82_002995"
     CDS             10209..11501
                     /locus_tag="KQD82_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG4381453.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         4593 a         1133 c         2190 g         3793 t
ORIGIN      
        1 tgtttagata tgattaatac tcttgatttt agaaataaat atttgtagat tgtagattaa
       61 agataatata aataaataga aggtagattt tttagatata ttaatataaa gattatataa
      121 aatattgaaa tatagctgtt taaattattg tctataaata taaaatgaat ggtggtgagt
      181 taagtgaccg atttagttaa caccattaat ggaattgttt ggtcacccgt attagttgca
      241 atgtgtttgt gtataggatt atatttttca ttgaaattaa aactttttca aataagagat
      301 gtaaaagaaa tgtttactct cttgcttgaa ggagatagtt ctcatgttgg tatatcatca
      361 tttcaaggtt ttgctacatc tatggctgga agaataggga ctggaaatat agctggtgta
      421 gctgtagcaa tagcaatggg aggtccagga gcattagtat ggatgtgtat aatggctaca
      481 ttaggttcag caactgcatt tgtagaatct actttagctc aaatatataa agatgagcat
      541 gatgggcagt atagaggagg acctccatat tattttgaaa aaggtcttgg gtggaaaaag
      601 tgttcagttt tattttcaat agttgcaata ataagttata cattttttat gcctggtaca
      661 caatcaaaca ctttatatct agcaatgaat caagcatttg gattttcaaa aatgacaata
      721 gcagtagcat cttctattgc attagcgata ataatttttg gaggaattaa aaagattggt
      781 ttatttgctg aaaaagtagt acctataatg ggtggagcat accttattat gacattagta
      841 ataattataa caaatattag tttagttcca gaggctattg taacaatgtt taaatctgca
      901 tttggatttg gttcagtttt tggaggaatg ctaggttcag caatatcttg gggagtaaaa
      961 agaggtatat actcaaatga agcaggagaa ggttctggga cttatggtgc ggcagcagca
     1021 gaagtaagtc atccagccaa acaaggatta gttcaagcat tttctgttta tgtagacaca
     1081 ttattaatat gtatggctac aggtattatg attgtaataa caggaatgta taatgttaca
     1141 ggtgtttgca cagcactacc aggtgtagaa ccgggaccac aatatgtaca gagtgcaata
     1201 aatacattac atccacaatt aggtgatatt tttatagcaa tagttttatt attatttaca
     1261 tttacaactt tattagcctt ctcatatatg atggagacta atgtatctta cttagtaact
     1321 agaattaaag cagaaaaaca tgaaaagtac ataattaatg tctgtagatt tatgcttata
     1381 gcatgtgcag caatgtgttg ttttatagaa ccacttacaa gctgggcatt aggtgatata
     1441 tgtataggtg tattaacata tataaattta attgccatct ttacactaaa aaaaccagct
     1501 ataaaggcat attatgatta tataagacag aagaagatgg gaataaaaag gagtgataga
     1561 acttttaacc ctatagagtt agatataaga aatgctgagt tttgggaaaa tagatactta
     1621 aaagaggata agcatgagtt aaattaaagt tataataaaa tataaaaatt actaaataaa
     1681 tttagtggga ggtaagattg gtatgaaaga aagaataata caagtggctg attcaaagaa
     1741 agaaaaaatt ctagggctat gtcaatcttt atatgatgaa ccagaaattg ctttgcaaga
     1801 atataaatct gctaaaaaaa tatcagaatt tctaagagaa gagggttttg atgttgagga
     1861 aaacttggca ggtatggcta cagcttttaa agcaaccaag aaaaatggag atggtcctaa
     1921 aatagcattt atagcagaat atgatgctct tccaggcaat ggacatgcat gtggacatca
     1981 tttaattgca tctatgggag ttggagctgg aatagctctt tcaagtatac tggatactta
     2041 caagggagaa gtaagtataa ttggaacacc tgctgaagaa actggagatg gaaagcctta
     2101 cttgatagaa catggtgtat ttgatggcta tgatgcagca atgatgatac atccaaattc
     2161 aaaaacttgt gtaacacctg aaattatagc aataggaggt ctagatttca tatttactgg
     2221 aaaagcatct catgcaggag ctaagccata taacggaata aatgctttag atgcagtagt
     2281 actactatat aataatataa atgcactaag acaacaatta gtagatggaa cgagaataca
     2341 tggtattata ttagaagcag gaactgctgc aaatgtaata cctgatatgg gaaaagtaag
     2401 attagagata agggcaaaag aacaaaatta ctttgatgaa gtagtagaaa aagtgaaaaa
     2461 ttgtgctaga ggtgctgcaa tagccactgg atgtgaactt gaattttatc actttgaacc
     2521 aacttgccaa ggattaaatg aaaataaagt tttggtagat atatttacaa agattatgga
     2581 ggaatttgga atatatgaag atgaacaagt aatgttaggt tcaacagata tgggaaattt
     2641 aagtcaaata atgccatgta tacatccttt gatgaagttt tctgaaaatg gagaagaact
     2701 acatacaaaa gaatttttgg aggcttctat aaattcatat gcaaaagata gagtaattga
     2761 tggtataaaa atattagctt taacaggatt taatttattt gaaaatccag aattattgaa
     2821 aaaaatgaag gaagagtaga acttagacta tttttaatta tactctttaa aataagaaga
     2881 gtgtctcaaa atgaactttt tagtttatga gacactcttt tttgtatgaa attaaatata
     2941 tttttattat tttaaacatg aaaaagaggc ttatctgttt ttatatgacc ttttttaact
     3001 tgtagcacaa aatatcatga atgattacaa ttcgaatgat aaaatcgatt ataagatata
     3061 taggaggggt acatatggaa actttagaag taagaaatat tgaattgagt ggtacaaatt
     3121 atgagatagg atacagacta ggagaattgg ttgctaatat gccagagata atagaaggtc
     3181 agattaataa atctaatctg gtaagcaaaa aagaagaaaa agagatgata gagttgtttg
     3241 ataaatattg tccaggtctt aatgaagaat tgcaaggttt tgctgatgca attcaagtga
     3301 attgtaatca aattttatat tatactatga cttatcttaa accaggttgt agtcaagttg
     3361 cattagcacc agaattaact gaaaatgggc atgtactatt tgcacgtaat ttcgatttct
     3421 cacacaatat ggaggatttt gttttatgta aaactaaggt aaatggaaaa tatgcacata
     3481 taggtactac cattatgcaa tttggaagag gtgaaggtat gaatgaatgt ggcttaggtg
     3541 taagtcagtc atcatgtggt attcctgtag gcaattcaga tggtttaaga aaaccagcaa
     3601 ttgtaggttt acagttttgg gctgttatta gatatttgtt agaaaattgc aaagatgtag
     3661 atgaagcatt agaatattta aaagatatgc caattgcata caatattaat ttattgcttg
     3721 cagataagtc tggaaacatt gcacttgtgg agactttaga tgggaaaaaa gaagtaaata
     3781 taatcaacag tttagaaagt aaaagagaat attttttaca ctctactaat catacacata
     3841 ttgataaact tcataaatta gatccacaat caatgaaaaa ttctattcat agatataaat
     3901 taattaaaga atatataaat aagtctaaaa aaattggaga gaaagagtta atgaatttat
     3961 tgtcttcaaa atatccaaat ggcttatttt gtaattacta taatgatttt tttggaactt
     4021 taaaaagtat agttatggat ttaaatatag gaaagtttaa tattttatgg ggtggactag
     4081 agaataaatg ggagagctac tatttaaaaa atgacattaa atctataaca cagaggataa
     4141 acataaatat tgaaaaagca ccatctgact tttttgattt cattgaataa aaaatgtatt
     4201 caattattct aaataattag gatatataaa tcaactaaaa gtttttattt atcgtattga
     4261 attagttata atattttagt atagtaaaaa atagtgctat tttacatatt taaagagttt
     4321 aagtaaaaga gaggtgccat attggattac ttaaagtatt ttgaaaaagc tgtaatgtat
     4381 attgagaata atttacatga aaatataact gtaaatgatg tggcaaaaga aactggatat
     4441 tcttattatc atttaacacg cttatttaag tctatgtttg gtgagagtgt aggaagttat
     4501 ataaaaaaaa gacgattagt aagtagtaca aaagaacttt tatatagtga taaaaaagtg
     4561 atagatatag ctattggtag tggatttgaa tcttcagaag catttagcag agcatttaaa
     4621 tctatataca aggttagccc tatagaatat agaaaaaata gaattgatgt atttgttggt
     4681 aaaaagaaaa aattagagtt agattttatg agacatcttg ttggaaatat aacgattaaa
     4741 ccagttataa aagaaataga aaaaataaag gtaataggta taaaggataa agtaatttta
     4801 gaaaataatt cattacctga cttgtgggag aaattcagaa aagtacatca tattgtccca
     4861 aatactttgc catcaaagag agtttttgga atctgtgaag caatctctga aatacaccta
     4921 gttagtgaaa gtatggaatt taatgagata ataggacttg aagttaatag ttatgatgtg
     4981 attccaaatt catttgtttc aaaaactatt aaggaaggaa agtatgctat atttactcat
     5041 actggtaact tagataattt agacaaaact tatgaatata tatggggtac ttggttttta
     5101 agttcaaaag aagaacttga tataagagat gattttgaag tatatgatga acgattttta
     5161 ggtcctagta atgtgaactc acaaattgat atttatatac ctataaaata atctataaga
     5221 aaactaaaaa ataatttgat taagaaatag atgttcgtaa aagagcatct attttttatt
     5281 tttataagat ttagataatt ttaataataa aagaaataaa aataagttct taaatttcta
     5341 agaagaattt agaatatagt cgaaataaga attgtatatt tctgaaaatt ctaattagta
     5401 caattttttc ctttataaaa ttgaaaaaag atgtatttat ttatcaaaaa tattatatta
     5461 aaatgatatt aataaaaaaa catttacaat attagcatat agtacagaaa aaagatgtaa
     5521 tttgtaaaat ttatggtaag cagtaaaatt aaaaatttaa agatattata gggaggttat
     5581 aattttatgg aaaacgttat tacaggtcta agtaaaggtg cagagtggtt tatcggtttg
     5641 tttcagaaag ggggagaaca atttataggt cttgtttcag gtactcttcc aacgctaatt
     5701 gtattaatgg tagctattaa ctctttgatt aagataattg gtgaggaaag agttaataat
     5761 tgggcttcta agctaggaaa gaactctttt acaagatata ttctacttcc attagtatca
     5821 gtatttttct tgggaaatcc tatgtgctat acatttggta gatttttaaa agaagagcac
     5881 aaagcaggat tttatgatgc agcagtttca tttgtacatc caattacagg tctttttcca
     5941 catgcaaatg caggagaact ttttgtatgg ttgggaatat ctgcaggttt gactacttta
     6001 ggtaaagaaa ctactacatt ggcattgtgg tattttatag ttggtctaat tgtaattttt
     6061 ataagaggta tagtaacaga aaaaatgtat gcttttctaa caagaaaaaa tagagcaaca
     6121 aataaggctt aggaggtaga taaaatggag gagaataaaa tattaaaaat agaaaaggga
     6181 acttcaggct ggggaggtcc tttgtatata aaaaaagaag ggaatagaaa taagatacta
     6241 tcaatgactg ctggtggtat ccatgaagta acattaaaaa taaaagaact actgggttgt
     6301 gaaattgtag atggatttaa aactggagtt tctgatgaag aagtagcagt agttattatt
     6361 gattgtggtg gaactgctag atgtggagtt tatccaaaga aaaaaatacc tacaataaat
     6421 gtaaatccag ttggaaaaac aggtccttta gcaaaattta taacagaaga atattatgta
     6481 tcagatgtta atcctaactg tataagtgtt gtagatgggg aagatatgcc acaaaaatct
     6541 caagaaaata aaagcgaaaa taagagttct attagaaagc ctgataatta tgatgaggtt
     6601 aagtcaaaag cacaaggtga atatgctaaa aagaacataa tactttctat tggtcaaggg
     6661 gcagggcaag tagtatcaaa attttatgat gcaggtcgag atactatcca aatggtaatg
     6721 aataacgtaa taccatttat ggcatttgta agtatgctta tgggaattat tttagcatct
     6781 ggactgggag actggattgc aagagttata tctccacttg caggtaatat tggtggatta
     6841 ttgattatat cagtaatttg cacacttcca ttcttatcac caatactagg acctggtgca
     6901 gttatagctc aagtagtagg gactcttgta ggtacacaaa ttggtcttgg tgcaatacca
     6961 gcatatttag cacttccagc tttatttgca ataaatgggc aagctggatg tgattttgta
     7021 ccagtaggat taagtttggg agaagcagaa ccagaaactg tagagtatgg tgttccagct
     7081 ttattttact ctcgtttaat aactggtcca atagctgtta ttattgcata tggagttgca
     7141 gtatttgcat tgagataata tttaatgtca atataaggct tggaattaat taataaaatt
     7201 gtaaatataa atttaagcta gtcaagattt tttgttaatg aaagactagc ttttatacta
     7261 ttaattagct gtaattatcg agttagatta atactaaatt ttcctttatt aaagtgaaat
     7321 tttatgtatt aatttggttt caaatttgta ctagaatgaa atcaaggagg agatacaaaa
     7381 tgagtgtaac tgagaaaatt ccttctattg accgcaagat atttaagata ttgattatgt
     7441 gtagtaaaaa agagtttgtg agtattaact caatagcaaa tgaattgaat gtcacaacta
     7501 gaagtgtaag gacgtatata aaacaattga ataaagattt aggtaatgat atagcagtaa
     7561 tcaaatatat aaagggtcaa ggctataaac tagaaataaa agatgagcag atactcaata
     7621 agattattga tatgaataga aaaaatgtat tttcattaaa ttcaaaagaa gatagagtag
     7681 agtttatact aaattacttg atagaactag atggatttat tacacttgat agtttagctg
     7741 atgagatatg tgtaggaaga actactttag taaatgattt ccagtatgta gaaaaagttt
     7801 tggcttcata taatttaaac ttgattaaaa aacagaatac aggtatgaaa ttaaatggaa
     7861 atgaacttga tattagactg tttatattaa atcagttgta taaaaactca agaaaagatt
     7921 tcagtaattc aaagtatttt aaaggaatca aaaaagaaga aatcataaat ttagaagaaa
     7981 aactattgac tttatttaaa aaaaataatt tttatgttac agatgaaatg ttaagagaag
     8041 ttattaacta catagtagtt ttagtctata gagtaaaaga agaaaagaaa gtaaaagatt
     8101 atgatgtaaa gtttgattta cttaaatcat atgatgaata ctttattgct agggaaataa
     8161 aacatataat atcaggaatg tttgaatgta ctttaaatga tgaagaaatt atttatttaa
     8221 ctataccgtt agttagtgga aatgcaccag catcagaatg tgctttaaat agtagtagaa
     8281 ttagtaaaaa cattgatgag ttgatggaaa atatatttaa tcaaatatat gtcgatatgg
     8341 gtatttttat aaatgaggat gaattaagag taggtctagg atatcatctt agttttactt
     8401 taaacaggct attatttaat ataaagttaa aaaatgtttt gttagaagag attaagcaaa
     8461 attatatact tccatttaaa ttagcacaaa tagcaggaaa agtaatagag aaaaagtata
     8521 acttagaagt ttcagaagat gagattggat atatagcaat ccatttcagt ggatatttag
     8581 aaagaaatag cagtagattc tattcaataa aaaaaatagc aattatatgt agtacaggtc
     8641 taggaacagc aaaactattg aaaataagag tagaaaaact gataggaaat aatcctaaaa
     8701 ttgatactct ttcaagtttt aatttgagaa atattaactt agatgaatac gatatagttt
     8761 ttacaactat tgatttagac acatcaaata taaatacaat agtattaaaa ataaatacaa
     8821 tatttgatga aaacaaacta agagaacaat taaaaacagt attgtgccta agagaaggga
     8881 atattgacac tactaattct acaaatttac taatcaataa tttgttagat gaagataaat
     8941 ttatgatatt aaatgaaaag actatacttg cctcattaga aaaaatgatg gaccatctta
     9001 tggatttggg atgtattgat gagaagttta gaaaaaatat agtcaataga gaagaaaaat
     9061 ctcccacagt atttgataaa ggtttattat ttcctcattc tgtaaatgaa aaatcagata
     9121 agtttttaat ggcagttgga attttagaag aaccaataga atatgcaaat agaagtataa
     9181 aaataattct tatgacaatg tttccatgtg agaataagat agactcagac ttattagtaa
     9241 aaatttatga agaagtttta aagataggcc aagatgtaaa attaacgaat aagataagta
     9301 aatgtagaac ttttttagaa tttaaaaagg tgttacttaa aaaccttata taactattgt
     9361 acagcattta tagttgttat acagatttaa aaataatgtg atttttggaa ggtgtataaa
     9421 tatgaaatta ttagtaattt tatttgttgc ttatatttta aatactatat ttataatgaa
     9481 gcagaataaa ttgtatttta aaactttaaa tagagctaaa aaaaagggtg atatagtgtc
     9541 tacaggcaaa aagaaaagct atttttctaa aggaagtata gccgttatat cttcagattc
     9601 agatgggttt ataaagtttg gagaaatttt aaaagggcgt acagtaatgg ctaaatttaa
     9661 agagattgaa gaaatagaag gattagatat atatagtgca gagcaaaaat ttaaagatga
     9721 agagagtatt gtacaagcta taaaatttat aaaagaaaaa ataaatgttt cttttaatta
     9781 actcaatata aatataaatt atcaggagga atgtcatgaa atatgaagtt aagataagtg
     9841 ggataggcag tttagttaaa gagttaatgg atgaaagcaa ttgtcttatt atatatgatg
     9901 aaaccattaa tgatgacgac ttaaaggata tatctgtaat tcattcaata gcaacgttaa
     9961 aaagtgatgt tgaaatagga gatacactta ctatagggaa tagagattat tgtatagttt
    10021 cagttggtga tattgctcag aagactctta gagaaattgg gcattgtaca atcaaatttg
    10081 atggaaaatg tgaagtaaat ttaccaggag aaatacatgt ggaaatagga aatccagata
    10141 taactattgg agatttaata actatttctt aatttgaaaa taaaattatt ataacaggag
    10201 gaattggtat gttagattta aacagaaaac ttgcaaaatt ggaagaggaa ggtaaaaaaa
    10261 taaaagtagc tcttatagga gctgggcata tgggaaatgg aatggtaagc caaatggcaa
    10321 atatgaaagg tattgaagct tctattgttg tagatataaa tttggagttg gctcataaag
    10381 cttttactga tgcaggaata gatgaagaaa ttatagataa tgtaacaaat gaaaaggatg
    10441 cagaaataaa attacaagaa ggaaaagtat tgacatgtaa tgactttctt gtggcttgta
    10501 aaactaagtc aatagatgta gttatagatg caactggggg tattgcaatt ggtgcagaga
    10561 tagcattaaa ctctatttta aataaaaaac atatagttat gttaaatgct gaaactgatt
    10621 gtgttgttgg accaatactt aaaaaattag cagatgatgc tggagtaata tttacaggtt
    10681 ctgcaggaga tgagccaggt gcagtaatgg aattatttga ttttgcagat gcaatggggt
    10741 ttgaagttag agtgataggt aaaggtaaaa ataataaatt agatttagat tgtaatcctg
    10801 atacagttag agaagaagca gaaagaaaag gagcttcacc tcatatgata gcttctttta
    10861 aagaaggaac taaaacaatg gtagaaatgg ctttaatgtg taatgcaact ggatttgtgc
    10921 ctgatgtaag aggtggtcat ggtattgaag ctacagttaa tgaagtacct aagaaatatg
    10981 cattaaaaag cgaaggtgga gtattagata attacggtgt agttgatttt gtaaatggta
    11041 tagcacctgg agttttcgta gtagtagccc ataaattaaa agcagttaat gatgaactaa
    11101 aatatttaag tatgggtgat ggacctaatt acatattata tagaccatat catttatgta
    11161 gtttagagac tcctttatca gcagctatgg cagttttaga ccataaggct acaatagtac
    11221 ctaaagctgg actagtagca gaagttatga ctatagctaa gaaagatttg aaaaaaggtg
    11281 agtttatgga tggctatgga gcatatacat gctatggtac tatagaaaaa tacgatgtag
    11341 caaaggctat gaatgctgta cctattggtt tgataagtaa aaagactaaa gttgtaaaag
    11401 atattaaaaa aggtgaagtt ataacttacg atatggtaga gatagaaaaa gatactactt
    11461 tatatcattt aagacaattg caagaaaaaa tatttgggta agatttgatg gtaatataaa
    11521 tagatattag taaaaagggt tgatggttat ttttaaatca acccttttaa atttttatat
    11581 attcttgtta ggatactttc tttaaaagta tattataggt tatttgtagt cgtaaaaatt
    11641 attttgaatt agttctgcaa ttttatattc acattgaaat aatattatta aaaattttaa
    11701 tataaaatg
//
LOCUS       DAENCV010000113        11651 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAENCV010000113 DAENCV010000000
VERSION     DAENCV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.114"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(24..2090)
                     /locus_tag="KQD82_002996"
     CDS             complement(24..2090)
                     /locus_tag="KQD82_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG4381454.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDENDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDIWKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2167..5196)
                     /locus_tag="KQD82_002997"
     CDS             complement(2167..5196)
                     /locus_tag="KQD82_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4381455.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5249..6709)
                     /locus_tag="KQD82_002998"
     CDS             complement(5249..6709)
                     /locus_tag="KQD82_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG4381456.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFENFKAELENAIKK"
     gene            complement(6801..7175)
                     /locus_tag="KQD82_002999"
     CDS             complement(6801..7175)
                     /locus_tag="KQD82_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381457.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7351..8091)
                     /locus_tag="KQD82_003000"
     CDS             complement(7351..8091)
                     /locus_tag="KQD82_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG4381458.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8112..8900)
                     /locus_tag="KQD82_003001"
     CDS             complement(8112..8900)
                     /locus_tag="KQD82_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG4381459.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(8988..9078)
                     /locus_tag="KQD82_003002"
     tRNA            complement(8988..9078)
                     /locus_tag="KQD82_003002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9042..9044),aa:Ser,seq:gct)
     gene            9286..9837
                     /locus_tag="KQD82_003003"
                     /pseudo
     CDS             9286..9837
                     /locus_tag="KQD82_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
     gene            complement(10663..11175)
                     /locus_tag="KQD82_003004"
     CDS             complement(10663..11175)
                     /locus_tag="KQD82_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381460.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         3728 a         2014 c         1125 g         4784 t
ORIGIN      
        1 tctatctaaa tttagattct aaattaaata gtataaatta tattcttcaa taaacaagtt
       61 ttcaaatcca ggattcgctc tctatcttta aactttattg taaacataat tccatctttt
      121 ttaattattt ctccttcatg aaatttttta tggtatacct tatctcctac accaatactg
      181 tcaatttctt ttttcgttgg agattttata tcattaataa ataatgattt atcaactttc
      241 ttaccatact tatttaaagt agagcttata cataactttt cttcagctct tgtaatagca
      301 acatacataa gtcttctttc ttcttctaat tgttcttctt ttttctcttc cttccaaata
      361 tcatatgatt tttcatgagg tattgttcct tcatttacac ctataatata aacatttcta
      421 aattctaatc cttttgcact atgcatagtt gtaaatatga caccctcagt ctgcttattc
      481 ttgttatttt ccgttaactc agtctttact ctatcaatat gctctaaaaa ctctgtaaca
      541 gtattaaaat tagttgctga actctcaagt tcatttaata tctctacaag tccattagtc
      601 tttatctttc tgttgctaca ataatctaaa acatatctat cataatcaag actagttcta
      661 atataagata ttgcattttt aggatttaaa gtatttaaat aacttaaatc tatttccaaa
      721 tcatttattg ttttaacttg tttaggatga agattacact tatttattaa tgctgttata
      781 aagtcatttt catccttaac catatttata ctgtctttgg atatatatct aaaaggcttg
      841 tttataattc tcaaccaatc tttatttgat tttggattaa tacctattct taaataggct
      901 agtatgtctt gacttgccca atggtcatat attgtaatta ctgagtcttt tactacaaat
      961 ggaatcctca tatccataaa tacatctact aatgctcttg actgtatatt agttctataa
     1021 attactgcaa aatctgaata ctctacataa tctttttgta tttcgtcaat tatttccctg
     1081 gcaatatata aggcttcttc ctctgaatca tgcggagaaa tataggtaac acttcctcca
     1141 tccccctgac tacacttaat gattttttca tatctatttt tatttttgtc gataagtcta
     1201 tttgcagtat gcactatctc acttttagac ctgtaattta tatctaatat tattttttta
     1261 gtattattaa agtattgttc aaattcaagc aaaaaatctg gtctagcacc tctaaatcca
     1321 tatatacttt ggtcttcatc tccaactgca aatatattgt ttaatgggct acaaattaac
     1381 tttaaaactt caaactgtac tttatttata tcttgaaatt catctatcaa tatatattta
     1441 tacacatttc taaccatttc tagtgcagac ttattgttca aaagtaaata ataagttctt
     1501 ataagcatat catcaaaatc tattttatta actttagatt tttgttcttc gtataagttg
     1561 tatactttta aaaactcatc ttttgttaat acttctgaat taaattcatt tttatccata
     1621 agttcatttt ttacatagga aatttcattt ataacttgtc ctatattctc atcatcatca
     1681 gcattctcta tatttaaact ttttaggatt gccttgattg tcattcgctt tgacttttca
     1741 tcaaatatac tgtctaagtt gtatctttca aagtatctta gaattttaaa aaatacagag
     1801 tgaaaagtac cataggttac cttattaagt cttatatcat cacttaaatt caaagctcta
     1861 tttttcattt ctattgaaga tgccttggta aaacttattg ccaagattct tgttggtgct
     1921 atgtttttgt ttacaaccat attggctatt ctatatgtaa ttactctggt cttacctgaa
     1981 ccaggccctg caagtatcat gcaaggccca tctatatgct caactgcttc taattgattt
     2041 tcatttaact ttcttatatc tatatttgtt gtgattatta gctccaccac cttgatttat
     2101 aattatatta ttttcttttt aattataaac taaaagttca taaaaataaa tttaattttt
     2161 cctctattaa accacatcat tcctattttc atgacatatc ttgagttctt ttttaatttt
     2221 gtcgcttata tcttcaggta taaatgatgg tttactaata tagtagcctt gcatataatc
     2281 tactccaaga ttaattagtg tttccatttc accttttgtt tctacacctt ctgctatcac
     2341 tttaatattt ctttcgttag aatatgagat aagatttttt aatattttct gtctgttttc
     2401 atctttatca atacctctta caatagacat atcaatcttt acatagtctg gcattatata
     2461 taatagtaca gcatctccgt tgtaacctgt cccaaagtca tctaaagcaa tttctatatt
     2521 ccaatggtct atatatgctt tctttttctc aataaaatct tcattaatct tatcattctc
     2581 tgttatttct agtactactc tatttaaata ttcaccatat aaatcttcaa tttcctcaaa
     2641 atctactttt gacaaattat tattaggaat agaatttaag aacagtttgc aatcgccaaa
     2701 tttttctttg tattttttaa atgatttaag tgctttaaac catgtaatac gctcaatttg
     2761 atataattta gactgtgaac gagctaatct aatgacatct attggtgaac taaaagtttt
     2821 tattttaggt ctcataagtg cttcatatgc aaaaacttta cctttacaat ctacaatagg
     2881 ctgaaatgca tactcaacta attgaccatc aattaacttg tttaattctt ctctattact
     2941 caataagaat gaatccttat tatatatatt aatatcaaat tcactaactt cacccttgat
     3001 tgtattttta atttgataca tagcaaaatc tgcgtatctc attaattcat catagttggt
     3061 tgaatcatca gggtaccagg aaattcctgc tgatgccctt attttaaatt ctgttccatc
     3121 atgaagtttt aataaggtat tattcatctg tttcttaaca gaattaataa tatctcttat
     3181 actatcttta ctttcatatc catatataaa tacataaaac tcatcacctg aaagtctaga
     3241 tactatacca ttccatgata taaacttctt aagtatgtct gcagcacacc taatatattc
     3301 atcaccagca tcatgtccat atgtatcatt aatatatttt agattatcta aatcccacat
     3361 aataaatgct gctatcttaa gttcatcttt attcttaaat ttttcttgta tctgtgaatg
     3421 aaaagctctc ctatttaaaa gattcgttaa aatatcaaaa tctctttcat attctatctt
     3481 ccttttctca ataaattctt ttgttacatc aacaacaaca ccaagaacct ttagctcatc
     3541 ttcaaagata ttcattctta tccacttaag ttttttatct ttagattcaa acttatatat
     3601 atatgtattt tctgtttcat aactttcttg caaatattta tctagtcccc ttaatttttt
     3661 caggaactta tttttttcca tatatccata ttcttcaaat ttgatttcag tagtaaacac
     3721 gctaaaaaat tcttttgtac aaaaaacatt ctctgcgttt ttgtataact caaaagctcc
     3781 aattggcata tctaccattt caataatctg tgataatttt gacgaagaat ctgcaacatt
     3841 tgcactgagc aactctatag ctgaagataa ttcatctatt tcagatatat ttatcttatc
     3901 aagatttact ggttttgatg gattactgtt tctaactttg ttggctaaaa gaacaattgg
     3961 ttttgtaaat aatcttgatg caatataaat acctattaga cctattaata aagaaattgc
     4021 tatagatatt ataactaatt tctctaccct ttttgaaaat gacaaaagac ttttttcttc
     4081 aataataccc aaaagagccc aacgttcatt ttcaaatgga gtattggagt tatataaatc
     4141 taagtattgt acactagcat aaatgttgcc attaccacat tcattatctt tatttttaat
     4201 cttatatata tttttatacg attccttctg ctctaaatta gaatgtaaac catcccaaaa
     4261 tttttctttt aatgagtatc catttgaaat tacattttca aaggccatat cattatttct
     4321 atctacacct aaaatataat tacccttttt attcccattt atctctgagt atggaagtaa
     4381 ttctcttaaa taatttacac ttaaatctat tccaagtact ccatatacag ttccatcttc
     4441 ataaataagg ggaactgtat atgtcataag cctatcacct gcatttgtca ataaaaactt
     4501 acttccccag tatcctaaat tagaacttga tacatttgga ttatcaattg cagctctaaa
     4561 tggtttataa aaaaaatcat ctccatcttg ctcattttca cttttaaatg aaaacttagg
     4621 ttcccaataa ctgtccatag atattccaaa acttcttgct atatctgatg aacctctctc
     4681 aattaacaaa tcagaattat catttggatt aaacttaggg tctaaatccc ttatatacaa
     4741 tcctgtccta cttatttcac ctttactatc tattgactta tcgttaatat ttttgtcact
     4801 taggattata aaagctcctg taacagaatt ttttctcaaa agatatatta aatcatttga
     4861 tatgcttttt ataatatctt tgttaatttc ttcattgttg cctacatcct caattaaggc
     4921 atttttttca tttaatactt tttttacaga ggaattaatt gtagcttccg tttcacttgt
     4981 atttgaccat ctttgtaaca tttcattttg tatataattt ttacgattaa ttactctttc
     5041 atttaagatg tcaaacgaat tatcatttaa tttcttaata gttcctcccc ataaaattgt
     5101 ggcacaaaat aaacttgttt gaattagcat aacaataatt aggggaaata tcatcttttt
     5161 cattattgaa ttgttttttc ttagattgtt ttccataccc attccaccta tacctttgtg
     5221 ctcgtcaaca cactaactgc caattcatct atttttttat agcattttca agctcagcct
     5281 taaaattttc aaaccaagtt ttaaaatttt tatcattatt aaattgtgct actgcatcct
     5341 tcttagaagt tccacttttt actaattcat ttattttatt tgaatcttct ttagctttat
     5401 caagcattga atattcaagt atttgtctag ctttagttcc accatcaaat gctttattag
     5461 tatataaatt acaactgtta atctgttcaa tagatgaggc tattgaacct tcaactttat
     5521 cagataaact caaattatct ttcttcataa cttcttttat aacatccaaa tcattcgttt
     5581 ttttctttac tggtaagtat ccagaagcaa tagagaattt tatattaggt tctttatctg
     5641 taaaccattt taaaaattct actgatgcat attcttcttc tttagtagat tttgttacag
     5701 ccattcctgc accttgttgt acagcatatt ttccttctcc tttaaaacgt ggtgcttgaa
     5761 aaacagatgt ttcaatatta taactctcat tatcatttag cattactcta tctggaaagt
     5821 atgctgctcc tgaactagaa cctacaagag ctaatatatc acctgttttt gcatcatcag
     5881 aacgaaactt accataagaa gcaaaatatc catttatata tggtacatag aaattatccc
     5941 atagtttttt cataactttt tcatcaatat ttagtgtaac tttaccatct ttaacagaaa
     6001 aaatttctac tcctagttgt ttacttccta taataatata atttgctaaa gcatctcttc
     6061 caaaaaatgc tttaccatca ttaggctctt tcgttaaact atctgtccat tcataatatt
     6121 tttttgatgt ctctactaat ccttcaattg tttttatatc atttatattt gaatttgtag
     6181 cttttgcaaa tttatcccaa tcagttttat ttaacatcaa aatttcagtt gattttgcaa
     6241 ttgggaaaag tttaaactca tccttagaat ttattctgcc ttcttctaca tattcgtcta
     6301 tgtattcatt taattcttct ttacttatgt acttatccat atctactagt aaccctaatt
     6361 tatccatttc atatgcagta tctgagtatg ctgcaaatat atttggtaca tcttcagaac
     6421 caactttttt atttgcagaa tctaaaactt tctctgataa atcaactaca ttaccttgac
     6481 tgaatgcttc aacaataatt cctttttcag cacctacagt gtcattaaat tccttaacaa
     6541 gcttatcaaa agccattttc tgctgtccat tataataatg ccaaatttgt attgatgtcg
     6601 gattttctgg gtctaactta taattatctt ccttctttaa actacatcct gtaagtaata
     6661 aaacaatcat tgatgtgata attaattttg caaacttaca cctattcata aaatctactc
     6721 cttcaaatta taaaatttat aatagcatta aaactgtaac ttcactattt atattaatta
     6781 tagaacgttt attgatatat ttaaacagga tttacaacat atttaaaagc aattaagaaa
     6841 catactatta caaatataga aaataaagct tttacataat ttttatttct aaatgcacca
     6901 aatgacacca atccttgttg aatagctact gtaattacta aaagtatcat tattatatta
     6961 agttgaacca gtttaaataa gtaaagcaat gaaaacacta ctagaagtac acataataca
     7021 atactagatt tattaataat atcatcaatc ttatctacct tcttagattg cttattaatt
     7081 ttatttaatt tttctttatt taaattctca acttttttat tatacctttc tcttgtttta
     7141 tctttatatt tagtattttt atttttattt cccatttatt ttccatcctc tataaatttt
     7201 ttaattgagc ttatacatta tttaaatatt atatatttaa gataccatat attcctacaa
     7261 aaaggtaact tactatgtat ctatttttga ttttcgcata aaaaagccat ctcctttaga
     7321 attaattctg tattgagaca gctaatttaa ctatttatcc tttgaaacat tttctacatt
     7381 atcatattta ctatcaacta aaactatatc aactcttcta ttttttgacc taccttcttc
     7441 agatgaattt tctgctattg gtctaaactc accatatcct acagcagata atttatctgg
     7501 tgctatacct gcattgtcaa taagaagttg aacaacattg gttgctctca taactgataa
     7561 atcccaattt gacttaaact ctgaattttt tatagacata ttgtctgtat ggccttctac
     7621 tctcatgtaa ctattcattt catttaacat cttacctatt tgaataatct tatctctaga
     7681 attatccttc acaatagctt tccctgtatc aaatagtatt gtatctttaa ggcttattac
     7741 taatcctcta tcttgaactc ttaatgatac tgaattagcc ataccatttt cgtttaaata
     7801 cttctgaatt tcttcaccaa tttttttaaa cttagcattt tgtaaagtat ctaaatcatt
     7861 acttcctggt tcaactactg gttctatctt gctattacca ccttcaataa ctccagaaga
     7921 accaccaaaa gcagaattta gagattgaga taattgttta tatttctccg catcaacatt
     7981 actcatagaa tacattatta caaagaaaat cataagtaat gttatgaggt ctgaataagt
     8041 taaaagccat cgctcattgt tttcttcttt ttcatcttca tcatacaaca taaacattcc
     8101 atccttttat attattcagc tctaccacca atttcttcaa gttcaattag ttcttttgaa
     8161 tttaaaaata tttttaattt ttctgtcatt aaattagaat tatcaccctc aagtatggat
     8221 aatattgctt ctatcaccaa aagtctttca cttacttctt gttgatttat agccttaagt
     8281 tttgatgcta ttggtaacca tattaaattg gcagaaccaa caccatacaa agtagccaaa
     8341 aatgcaacag aaatttgagg tcctaatgca tctggattac ttgaaagatt acctaaaata
     8401 tgtactaatc ccataactgt acctattatc cccagtgtag gtgcatatcc acctgctgaa
     8461 tcaaaaattg ctattccttc tttatgtcgt tttgatgtta tctgtatctg tgattccaat
     8521 atatttctta tagtaactgc atccactcca tctaccataa gttcaagccc ttttctaata
     8581 aaagggtcta attcatcatt ctcacttatt tctttgtcaa tactaagaag cccctctttc
     8641 ctagcctttg ttgcaatttc tttgaaatag attatatttt ctatgttatt aggcttttta
     8701 tttttaaaag ctaataacat tatctttata gactttttaa acacagggaa tggagtcgaa
     8761 gctccaacag ctcctatagt tccaccaaac acaacaagtg ctgatgaaaa tacaaataat
     8821 gctccaacat gtcccccatc aataacaaaa gcaactacca aagatgctat ccccactatg
     8881 aaaaaaatta aagttgtcat ctattccctc ctttctttag ttatacatat ctaatcattt
     8941 atgacattat aaaaagacta tctttaataa aaatagcctt taataattgg cggagaagga
     9001 gggattcgaa ccctcgcacc ggttacccag cctaatccct tagcagggga tcctcttgag
     9061 ccacttgagt acttctccat atctctatac tagtaaatta tataataaaa tcatatttta
     9121 gtcaattgtt ttataaaatt tgagttattt tgaattatta aacatctatt tttacttttt
     9181 tgcttaaatg taagttttaa atatggtaaa attatcttgc ataacactta taatattatt
     9241 tgataaatat acatacatta acaaatatac ctaaactctg acttaataga tataataaca
     9301 ggtgttggtg gtaaagctct tggagcattt ccaggttctg catacctttg tgctaaagag
     9361 agtattttac aaaatattaa agaaaatcaa tgtaagaatg tgtatcttaa tctttataaa
     9421 cactataaag ttgctaaatc atcttctcaa acaccaaata cacctaatgt tactttaata
     9481 tttgccctta atgaagcttt aactgagttt ttggaagatg acagtaaaat agaaagatac
     9541 aaagaatgtt ctgctatttt aaggaatggt atggaagaat tgggtctaac atttttactt
     9601 ccagataagt atatgtctaa tacagtaaca tctgtatttc tacctaaaga aatcaatgta
     9661 aatgagttta tattagggct agaaagggaa agtggctatg ttgtctatcc aggaaaaggc
     9721 aaattcctag atgctaacat attccaagta gcaaatatgg gagaaatata tccagaagat
     9781 tgttataaat tcttagatat cttaaaatca aaattggaga taaaaaaagt tatataacag
     9841 tttgtttgta aaatcctaca atatatcagt ataaattaaa tagactgatt tttttaaatt
     9901 ttcctataat aaattatatt ttatattttt gtataagggt gaactataat atgtaaagtt
     9961 cacccctatt tcaaatcttt ttccttcaat ataatctttt tcttttaatt tattttatat
    10021 ttttgacata attattctta catttaattg ttttttattt ttgacgcaat cgttttttat
    10081 tcagtcattt tatattcttg atacaatcat tcttacactt aattattttt tatttttgac
    10141 acaatcgttt tttgttgaat cattttatat tcttgataca atcattctta cacttaatta
    10201 ttttttgttt ttgacacaat cgttttttat tcagtcattt aatattttca acacaatcat
    10261 tcttacattc tattattgtt tgtttttgat acaactgttt ttattcagtc atttaatatt
    10321 cttgacacaa ttattcttac attctattat tgtttatttt tgacgcaatc gttttttatt
    10381 cagtcattta atactttcga cacaatcatt tttacattct attattgttt gttttgatac
    10441 aactgttttt attcagtcat tttatatttt cgacataatt attcttacat tctattattg
    10501 tttatttttg acgcaatcgt tttttattca gtcattttat attttcgaca caatcattct
    10561 tacattctat tattgtttgt ttttgataca actgttttta ttcagtcatt taatattctc
    10621 aacacaatta ccttttcatt caatcaaagt aatctttaag cattattttt caagctcatc
    10681 aaagtaatta tatacgataa cctcacctgt ctctttatct gtagtcttta taatgccctt
    10741 agctccattt acctcttctt ttgtaaattc atcatctagc acaactttga atttatcttc
    10801 ctttccataa ccttgttgaa attcatctct caaaattgca tttttttctg cttttggctt
    10861 caactcaaat tcaattccag atttcatttc atcaaaataa tttaatacta caacctcatt
    10921 attcatttta ttagttgtct ttataatccc tttagctcca tttacttctt cttttgtaaa
    10981 ttcatcatcc aatgttatat taaatttgtc ttcttttcca tattcctctt gaaaatccct
    11041 cttcatttca ttgtatttag tttcaaactc tttactcttg tttaattcca tagctgatac
    11101 atttgtcaat gatgctccaa ttgccattac tgttaatgcc attactatac cacttttaaa
    11161 tttttttact ttcatatctt acctcctaaa ttaaatgttc tcaatgaaat tatataaaga
    11221 aaatcctaag ttttacagtt tcattcttgt aactaaaatt actattatgt aaccaaactt
    11281 tttgcttcaa ctattcctta aaagttgctt ttttgaggaa tagttgaagt taaataatta
    11341 gattttatct atatttatag atttaaaatc aaatttatcc atataaataa atacttattt
    11401 ttgcaaaata aaaattttta attactccaa tccttcttat ttacttaaaa tcctatgata
    11461 agaaatttaa aagtctttac cttctttaac aataatttat ttacttttaa catacaaaat
    11521 atagagagta tatcaaaatc attttactga atattctaaa aataagtacc ccctattagt
    11581 atatatattt acatattaaa aaaaagtttt aaataaagtt atatttttta caacagtttt
    11641 gtaattaaaa a
//
LOCUS       DAENCV010000114        11635 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAENCV010000114 DAENCV010000000
VERSION     DAENCV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.115"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(467..1201)
                     /locus_tag="KQD82_003005"
     CDS             complement(467..1201)
                     /locus_tag="KQD82_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4381461.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYN
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2207..2845)
                     /locus_tag="KQD82_003006"
     CDS             complement(2207..2845)
                     /locus_tag="KQD82_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG4381462.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2848..3705)
                     /locus_tag="KQD82_003007"
     CDS             complement(2848..3705)
                     /locus_tag="KQD82_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381463.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3707..4081)
                     /locus_tag="KQD82_003008"
     CDS             complement(3707..4081)
                     /locus_tag="KQD82_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4381464.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5260..6450)
                     /locus_tag="KQD82_003009"
     CDS             complement(5260..6450)
                     /locus_tag="KQD82_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG4381465.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6466..8421)
                     /locus_tag="KQD82_003010"
     CDS             complement(6466..8421)
                     /locus_tag="KQD82_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG4381466.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKASTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSEPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8438..10504)
                     /locus_tag="KQD82_003011"
     CDS             complement(8438..10504)
                     /locus_tag="KQD82_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG4381467.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKDAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMNEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(10820..11470)
                     /locus_tag="KQD82_003012"
     CDS             complement(10820..11470)
                     /locus_tag="KQD82_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4381468.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT         3902 a         2027 c         1327 g         4379 t
ORIGIN      
        1 atttaaatat ttttactcac tctgcattat ggtacattat ttaaatattt ttcttcgctt
       61 tgcattatga tacatgattt aaatattttt actcactttg tattatggta cattatctaa
      121 atatttttct tcactctgta ttatgatata taacttaaat attttacctc actctacaat
      181 attatatata atttaaatat ttttcttcac tttgtatact aaagtttatt taaagtattt
      241 tctttcccat tatactatgc tgttaaatat ttttcaactg aagtattgta gaaccttaaa
      301 attactataa aaaactatat tctaaaatac ttgtaatact acatatcacc cttaacactt
      361 aagtataatt ttaaagcaaa attcttatta cttatattta attttgattc tacatcttta
      421 gatataattt taagtaattt aagaaattat tgtaaatcat atagtactac tcaataatta
      481 ggtttttcaa tgttccaatt atgtttcttt gatataatcc accatgacta aaaactatcg
      541 tttctatatt ataattactt atatttttaa tagcatcttt taactcttgt ttgttgctgt
      601 acatcacatc aacaggctta agttctccat tttctacctg taacaaatct ccagcaataa
      661 gtactttaga ctcttttaaa taaagacata tgtgacctct agtgtgacct cctgtatgta
      721 taacttctat ttcctcacct agattcaatt tttcatggtt atctaatgac acatctactt
      781 tagtatatga tgatttaaat ccaagtccca taacatttag catactaagc atcttatcat
      841 caattttatc taccatttgt tccaacatat aaagtttaaa aggagtttct tctcctgtta
      901 tatagctgac ttcacttttg tatgcatata ctttgatatt attcttagat ttttctctta
      961 gataatttat gtttcctata tggtctatat catgatgagt aattattata ttttttaacc
     1021 tatcaaagct cgtgttactt ttattaagag catcaattat taaatcacct tgacccggaa
     1081 gacctgtgtc aataagtgtt gcatcatctt ctgtgtagat tacaggaata tacataactt
     1141 tatcttcacc gaatacattt gatgaaattt ttaaaacatc taatcccttt gctaaattca
     1201 taatttttct ccttataatt acttaattag aggtcaattt aattaaagaa attgagaaaa
     1261 tgtagatttt cctctagtta tattttatta attaaaaatt attaatacct agtatatcac
     1321 ttaagttgaa ggattactcg acttgatttt tttgttattt tatggtatag taattaatat
     1381 agaagtaagt aattgcaagc tttgcaatta cttttatttt tttctaataa ctattttatt
     1441 tttgataatt cgtataaaat taaaagtaaa gtaatatgga aaatatgaat atagaaaaac
     1501 tttaataaga aaataaatta tgaataaata aaagaaacgc aatactgtat atagatgatt
     1561 atttagaaaa ataaaatctc ttttaataaa aataggcttc aaaataaact tttagttcat
     1621 aaagcttagg agtaactaaa ttgagtgcaa gtgtatctac tgttcaaaat aaatagttca
     1681 taaagcgctc ttttttatat taaaccaatt atattttcgt catttcagca atgaaaaaga
     1741 agcttatctc tattttgaga cagcttcttt ttattaactt cctacttttt attagttttc
     1801 taaaattgaa aagctttatt tataggattc cttagattta tattttgacg atataatttt
     1861 ttaaaaacat cttaaaaaat ggtatacaat acacacaatc atactcataa ttgaaaaata
     1921 atcaatggaa aataaaatta ctggatatta tctattaaaa aatacaaatt gattcacaag
     1981 aaacatataa tctataatat tatgatgtta ctctgccaca ccaagtaact ttattttcaa
     2041 gtaacctttt cataccatct aactttactc aataaaaatg gcaatataaa tatgtactct
     2101 attttttact tttaaatatc tgacaagctt tatatactat tggaagtaag atacaaagta
     2161 aaacagccca atctggcaaa tgtggcatat aaaagtcccc cttaccttat acaagctcct
     2221 tattactaaa aattttaata gaaataaaca ttgaagctaa cagtataatt aaacttaaaa
     2281 ggaacaatac aatattcact atcatggatg gatactgata tataaatgga atttcaaaat
     2341 tgctgtgagc tatagttaat ggtaatacaa atgatgaagc catgattatc accacgaaaa
     2401 agaattttgt tgtttcatat cctaatttat attgaatagg catataaact ccataaataa
     2461 tagaacttat taataaaaca atcaaaatat atgtcaagtc aaaagttcct atttgtggaa
     2521 taagtaaatt ttcaatccag taaactaatg tacaatagat aaataccatt gcaaataaaa
     2581 catacttaga ttgaactaag ccacgtctag gataaggagt tgaacaaagt aatacagatg
     2641 cttttgaata ttgactctcc tttaataata gatactgaca cagcataaat tctgaaaata
     2701 taattgatag tacaaatccc aatatttgtg caaatggagg taaacgccat aatataaata
     2761 atgggattcc tattgcaata aataatatca acaatacata tttttttaca atcagaatat
     2821 cttttttaac taaatgaatc aacataatta ctctctcctt tcaatatatg caagcataat
     2881 atcttcaatt gtaggacgct ctattaaaat atctggcata tttttttgca cttcattaat
     2941 ttttgaggta atccctataa aaccataacc tgtaatttct aaatttaaaa ataatttttt
     3001 ggtttcatca tttagaaatt gaatatcccc ttttactttt ctatgttcgt ccaatagagt
     3061 atctttttca cgctctaata taatacgtcc attgtcaatc ataacaatca tatctgctat
     3121 tttgtctaaa tcagatgtta tatgagtgga aaagaaaaca ccacgaccac atttcttcat
     3181 atatcctgtt agtatgtcaa gtatctgact acggattagt gggtctaaac cacttgtagg
     3241 ctcatccata attaaaaact cagctttatg cgataatgct aatgctaaag caaatttcat
     3301 tttcattcct tttgaaagtg tatcaatctt ttgttttggg ttaagattaa aacgcttcat
     3361 attttgttga taatcttctt cacaccaatt atcatatgaa ggtgcaataa tacttttcat
     3421 ttctgccatt gacaactcct catagaaata tgaatcatca aatacaactc caattcgatt
     3481 ttttaattcc ttctcatttc catcgaaatc tttaccaaag atttttattt ttccactatc
     3541 acgctttacg acatccaata tcgaacggat tgttgttgtt tttcctgctc cattaacacc
     3601 tataaatcca gttatacaat cttctggtaa tgcaaagctt atattagata atgaaaaatc
     3661 ggtataagtc ttatttaagt tataaacttc taaaatattt tccatattaa tcctcctgat
     3721 atagaatatc catcatttct tgtaactcta tctgtgtaat tcctagtgct tttgcatcct
     3781 taatcatatc tgccattttt tgttcaacta tacgtctctt tgagtctctt agtatttcta
     3841 aatttccagt tgaaataaat gttcctattc caacttggcg tgtaagaaag ccttcatttt
     3901 caagttcttc atacactcgt ctaatagtca aaatactaac ttttaaatca ttagcaaaat
     3961 ttcttatgga tggcattgca tccccttctt tcagttcttc agtaaatatt gcttctttaa
     4021 tttgctcttt aatttgttgg tataatggct tatctgatgt gttagaaatg agcactttca
     4081 ttttttatca cccccttttt atcattttca gtgtgatgtt gttatataca cactatacac
     4141 ttagagcact tttttgtcaa tataaaagat gtaaaatatt actatgacaa aataaatatg
     4201 aatgaaatat tagagatttt tgatagctat aaacactttt taaaaccaca taagatagga
     4261 tgtattaaat atttattaaa agtaagaatt gaatatctaa aaaaaagagg aatcctgtat
     4321 gaatgatttg tttgatgata aattaagtta taaactattt ataaaaaaca taaaggaata
     4381 aatccatttc taattacaaa catactaaaa aatttagata gtaataaata gatagtaata
     4441 aatgggatga atttataaga atttggatag aggaaagagt tattcaagag tgtagactag
     4501 aggtaaactc tatattaaaa aaatttaaat ttggatatta tgatgcatgg aatatttgca
     4561 gaagtaacct tgctataagt atagaagatt tttttgatta aaaaagataa tactctaaaa
     4621 tgtgagaatt tcaacattca atactgtgct ttaaataata tcactgcaaa ataccatcca
     4681 ccttttacca taattgaata ccctaattat tttaaaaaag aaaacattag aaacggaaaa
     4741 ccttgtatat ataaaataaa aaatactaac ttctaaaaca cgaatactct ttgataaata
     4801 aaacaaaaaa taattttaat aattaaaatt ggctatctca aactagagat aagtctcttt
     4861 ctcactttta aaactttgaa aatatattcg atttcgtaca aaaaagagtg ccttagaact
     4921 aaaaagttca ctttgtggca ctcccttttt tctcctcttt ttaattcttt ctaaagattt
     4981 tccaaaaata ttattaaata ccctatttat agataaatca agttaaagtt ttgttttaga
     5041 aaataaaaat aaactgtaac tattagaata gcaaataaaa aatctgtttc ttgataaaat
     5101 caagattata atgctaaaaa gaaaatattc ttataataaa ttaactaata taaaattcgt
     5161 ttataaatta atactgactt tctagcttta tttataaatt aatactactt tttagcttct
     5221 cctataaatt aacaccactt ttttaactat gaaacctatc tacaacattg gtaccataag
     5281 accagctacc acaatagaaa tcatacaagc cataccacca actaccagct taggcataat
     5341 cttagacatc aaatatgctc tttcttcttc cgtttctcca acagcattac acacttcatt
     5401 tgaaattaaa tatgtagttg ggaatcccaa catctgacaa aaccctactc caaaagcaag
     5461 aggtttagaa ccgataattt tccaacctgg taacaccttc atcataagga atataccaat
     5521 gatagaagct gcaaacatac ctacaattgg tacaaaaaga ctaattaaat ctgtcaaaga
     5581 aacttttgct aaagaaggaa taacagctgc aaacacaacc atgttgataa atcccttgat
     5641 ttttcctttt tccaaaatgt cttttggtac aataccaata cgtgttacaa taacaccaac
     5701 aacaagacat acaatagaat aatttacatg aataatctct gaaagttcat accctagata
     5761 agagaataat gctataaaaa atatacaaac atttgtagaa taatatttat cgtatttttc
     5821 acagaaagct tgttttactt ttgtgcctgt attctctgct tctttcttat cagtatcaac
     5881 atttgctaaa tcaatgcctt ttgattttgc ttcatgatat tctactaaaa gacggtttgc
     5941 ctcttgtaac gctgctcttg aagctattgg tgtaccaata aacttctgaa ttgcaaatac
     6001 aactgttgct gtagctgctg caagtgtcag tcctttttct agtgctgctt gtgtcataat
     6061 agtagtagca ggtaaagctc catttataac tggtatgctt gtcaatactg ttgattttcc
     6121 aaacaaagga atacataatg agacacagac tataactgct acaattccaa gccatgctgt
     6181 caatactgtt ctccattcgt ctataaattg tttaacattc aacattgttc ccatactaaa
     6241 cataatcatt ggaacactcc aactagctgc tgcggtcata cctgcttttt caataatatc
     6301 agctggtatt gcttttgtga caaatagaat caaaaataaa agtagtgtaa caaatacagc
     6361 tgatactttt gcttttgttt tgcttgcaat taaatcccca atagcaaata aaactaatac
     6421 gacactaaac cataataaca tatcactcat agaataaccc ccttattata aatatctttc
     6481 aaaccaatct acaatagcat ttgctcttgc aattctatta gatggtcttc catttctaga
     6541 caatccatgt gtatctccgt ggaacaaata catttctgta tctacaccaa gctgttttaa
     6601 tgctgtaaac atttggaaag tatcccccat aaaacatcta taatccctat cactatgtaa
     6661 gaacaatgtt ggtgtcttaa catttttcac atatttaata ggtgaatgat tccaaagaac
     6721 ctcctcatga ttccaaggcg tcttatctcc acaagtctgc ttcataattc tatccccaaa
     6781 gtcaataatt aaagaagctg taatcaagtt actaatacct ctttgagaaa ctgctgcttt
     6841 gaaacggtct gtatgaccaa ttatccagtt tgtcatataa ccaccgtaac ttccccctgc
     6901 aacccctaag cggtctgcat caatcccagg atatttttca atacaagtat ctacaaattc
     6961 cataagatgc tcatagtcaa cataccccca tctttctacc atttctaaga atttttcacc
     7021 ataaccgtca ccaccatgag ggttacaata aatcacatac attccctttg atgttacaca
     7081 ctgcttcaag tgtgtaaaca tatagccata tgcccattta ggtccaccat gaatgaacaa
     7141 tacagcagga tatttttcgc cttctttagc atctataggc ggtataacaa aaccatcaac
     7201 ttcttcttta ttttttgcta caaacgtcaa tttttcaggt aatgaaacct tatgagtatc
     7261 taaatatggt tcagaaaaag cagtcatttt tttcattgta cctgtagtca tatcaatgct
     7321 atatatttct gctaactcat aatctttcaa cattactgtg tacatggtgt tgtcgtaaat
     7381 atcaaaagat gtaaaagcat ctacttttgt gatgatacgt atattacctg tctttgtatc
     7441 aaactcatta aattctgtca tagttctgca aattcttgca ccaataaatt tgtctcctat
     7501 aactttatta ctacgtccac caccatatac tgcatctaca tccagtaatg tagcatacat
     7561 accatctgga ctagcaatca tatttatttc actagtactc aaatcaagta taaaaatatc
     7621 atgattctgt gtaatagaac ggtctaatgt tgaagcttgc aacattactt tatctttgcc
     7681 aatatatttt gccaaactta tttggtactt tccttgtggt acaagttctt tggtttcacc
     7741 actgtctata tgatactccc aaagtccaga aaaaggagtt gcacaatctt cataaatctg
     7801 accagtatat aaaattctgt ttttatcaga agaaacgtct acacctgcta cttcacaaaa
     7861 ttttggtgta atggcttttg ttatattttc accttcttga tataattcta atgttacacg
     7921 tcttctttga gaaaaatttt ctccatctct tataaaaggt ttatctgtaa atgtccaata
     7981 atctacaccc tctactgccc tatctggttc tgatttttta gaatccgttg gattttttgt
     8041 tgtcagtaac cacaatttat ttccaagtga tacaggtacg ctacaagctt ttggaataga
     8101 aaatgctttt tttgcttctc ctccttctat agaaatagtg taaaaatctg ttgtacttgc
     8161 ttttggctta tttcttttag atgtaaaaag tagttctgta ttagaaatcc aactaaatga
     8221 tgttacatct tttgtttgag tcatatgtat ttgtttttct gttttgatgt ccatcacata
     8281 taagtcatgt tcatagcagt ctttttcctc gttgatattt gatataatat aagcaatatg
     8341 ctttccatca ggtgaacaag aaatatcatg agggaatata tattcaaaaa tatctgaact
     8401 ttcaatcttt attttttcca tttttatatt ctcctttcta ctgtcctatg ttatcttttt
     8461 ttatatatac cgaaaaccaa tctagcattt caactaatct ttgtagcttt tgttttggtc
     8521 taccagaacg agacagttca tggttttctc cttcaaatag acaaagtttt gtaggacacc
     8581 catgaatttt caatgctgaa aacatctgga atgcttcttg catatagcat ctatagtctt
     8641 tttcaccatg taaaaataaa gtcggtgtaa cacatttatc agcataagca agaggtgact
     8701 gtttccattg aagctcgtgg ttttccatat gagaagcacc tgtttgtcct ttgcagaaat
     8761 aataaccaat atctgttgta ccctccatgc cactccagtt tgcaatagaa cgctgagcac
     8821 aagcaacagc aaaacggttt gtatgaccaa ttatccaatt cgtcataaat ccaccataac
     8881 ttccaccttc aacagccaat tttgttttat caatgtctgt atatttttca agtacagtat
     8941 ctgtaaattc cataaaatct atataatcaa tatctccaaa ctgtttccta atatctgcaa
     9001 aagcattgcc acgaccttca gacccccttg ggttacagaa gaatacaaag taacctgcac
     9061 ttgtaaatac ctgtatctca ttcataaaat atggtccata tgtcaatctt ggtcctccat
     9121 gaatcgccaa tacagctgga tattttttcc ccacctcata atctactggt ttatatacgt
     9181 atccttgtat ttcccattta gaacttcctt ttgactctat gtattctggc acagaaattt
     9241 tatattcctc taaaatatgg tcatttaaat gtgttttctt acacaatgtt ccattctcta
     9301 ctgtataaac ttctggtaat ccatttccta aacaacccac acatacaata ttgccatctt
     9361 ttacatctat aaaatcaact gaacctgtct gtgttacttt ttcctgtttt cctgttggaa
     9421 tatgtattct ttcaataatg cagtccatat ctactgtaga agtgtaataa atatattctc
     9481 catctacctt tgttgtctga ccagcaccca ttttaacatc agatgttact gtatttccaa
     9541 tacaatgatg ttgatgccct gaaagaagtt tcatttcttt cgtatcaata tcaatagtat
     9601 aaaaatctcc attttctcca tttccataag ataaagcatc agttaggttt agtatcactt
     9661 gattttgata taattcaaaa ccttttataa tatacttatc tttttctaat atacagacat
     9721 ttgtgtttgt gtttgtatca aatacataga ccccattttt caaacctttt acatctttaa
     9781 actcttgccc tatatataag atttttccat cacttatttt catacctaca acattaaaca
     9841 taggtgctgt aattgccttc aactcattta tattactgtc gtatacatat aggcgttttc
     9901 tttttctgct aatatatccc tccccattga cagcataagg aatttctttg aaatgttcat
     9961 aatgtctttt gttataagca tttatttttt tctgttgttc ttcttctggc aatccctcta
    10021 caataggtcg gtcattgtca taagaatcaa caactgcata caaacctttt cctataggat
    10081 tcaatttacc acctttgata gggagtctaa aaagttcttc tgcttctcca ccatttaaag
    10141 ataaagcata gtaactcatg cattgcttac cgtttttgac agcatctttt actttttcat
    10201 ttcgcaaagc agggaacaag atagtatttt catcttccca ggcatatgct ccaacagagt
    10261 ttatagacgt tatttgtttt aattcgccag ttttattgtt taatatataa acttggcttg
    10321 tgtaaccatt ctctttgata tcagcttttt ttacaataaa tgctgtatat cctccatttg
    10381 gtgatatttg gcaatcactt ataaattgat attccaaaaa tgtttcactc gtaaatagct
    10441 ttttcatagt attttctatc ctttctttag taattttttt caaactccct accacctctt
    10501 tcatttttta ttttaaattt aaaataaaaa attttaaatt taaaataaaa aattttataa
    10561 aggttatttt tttaggaagc cttctggctt ccttgtaata tttgtaattt aaagtatatt
    10621 taatccatgc ttaattcata cttaatctat acttagtcca tacttaatct atatttaatt
    10681 catacttaga cttatttaca ctatatttag attatattta ttagattata tttagattat
    10741 gtttaaacta taattagatt atacttagac tacatttagc aacttaatag ataagtatat
    10801 atagtaactt caaaacttat tattcatctt cacagaatct tttaatagtt ccttgaatat
    10861 gatttcttag tttatctact gctttttttg tatctctgtc ttcaatgaat tttaatattt
    10921 caaaatggtc ttgtactgtt ccttcataat tacgattttt taatggtcga attaaatagt
    10981 tcactactat aaatcttcct tgtataccaa catatacatc atttaaaatc tcattatttg
    11041 ccccttctac aatggcttta tgtaattcca aatctgctag tgcaagttct acagattttt
    11101 cttgacgata gtactcctcg catttatcag agtatctttt tattacatct agcttttgtt
    11161 gaaaaacctc ttcctcttct tgcatcattt tttctaaagc atatgtttca atcagtgttc
    11221 tcgtttcaca aacttgtttt accatatcat attcatactc tggtacgtac actttaccat
    11281 ttgtcataat agtaagcact ccatctttat ttagcatttt tactgcttgt acaactggtg
    11341 ttctgcttac ttcaaatttt tcacaaaggt catttagtaa tattttttga cctacagagt
    11401 attcaccacg aataagactt tcaataatct tatgatataa aatttcatct aaatttgact
    11461 ttactttcac tactctcacc ttatttctat tcttttgcca tattatattg aattactgga
    11521 ctttaattac ttttagtaat atttttaaag tagaaattct tatttgatag tacttttgta
    11581 ctacaattta aattattgta ctgtaaatca tgctcttata ctatagatta attca
//
LOCUS       DAENCV010000115        11549 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAENCV010000115 DAENCV010000000
VERSION     DAENCV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.116"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..1386
                     /locus_tag="KQD82_003013"
     CDS             <1..1386
                     /locus_tag="KQD82_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381469.1"
                     /translation="KKIHYEGINRDNSMLSSYKFNFIPDIILVIFLTSISFTIYVKSF
                     LGIFFILGLILLDLLVYSLIYALLKMVCLIKKIIKGKYSITFIYLNNDCKTNAFSSSL
                     LTITIIFVLLLFMISEITFAMFSNYEVDNSIKNSIVYQTTLEGKLITDKKLIENDIDY
                     FQYYPIKIDSIESVNNISVNDYIEKIPYENRKDVMNDLNDLSVDLFNTKQTILKSDLE
                     YGNWFKENDKENSIIINNYLHRYLIDYKLGDTVKLRINGKKIDFKVIGFLSPKSLNKG
                     VDLGYININENISPKTLDLSKNQPVIYFFKEKLDNTLLSKLLHNDKYANLEDYNDFAY
                     SMKTYVDDQKSLLLNIIFAVSLSSILLILTSQIIIFIRRVLDYQIMYRVGMSKRKLIK
                     IGLLEMIIVSIIQSTLIAIFYELFRFLLISLLQRAYNINYLLILIEFLVVLSINCFIF
                     ILAQRNIKLDL"
     gene            1900..2742
                     /locus_tag="KQD82_003014"
     CDS             1900..2742
                     /locus_tag="KQD82_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4381470.1"
                     /translation="MIINKILNNNVIITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            2887..4815
                     /locus_tag="KQD82_003015"
     CDS             2887..4815
                     /locus_tag="KQD82_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4381471.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISVGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            4876..6303
                     /gene="ascB"
                     /locus_tag="KQD82_003016"
     CDS             4876..6303
                     /gene="ascB"
                     /locus_tag="KQD82_003016"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG4381472.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFISA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            6714..7490
                     /locus_tag="KQD82_003017"
     CDS             6714..7490
                     /locus_tag="KQD82_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4381473.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAIALTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            7506..7871
                     /locus_tag="KQD82_003018"
     CDS             7506..7871
                     /locus_tag="KQD82_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG4381474.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(8038..8787)
                     /locus_tag="KQD82_003019"
     CDS             complement(8038..8787)
                     /locus_tag="KQD82_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG4381475.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            9265..9690
                     /locus_tag="KQD82_003020"
     CDS             9265..9690
                     /locus_tag="KQD82_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381476.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            9722..10909
                     /locus_tag="KQD82_003021"
     CDS             9722..10909
                     /locus_tag="KQD82_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG4381477.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTNIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            11101..11505
                     /locus_tag="KQD82_003022"
     CDS             11101..11505
                     /locus_tag="KQD82_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG4381478.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKGDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         4505 a         1184 c         1950 g         3910 t
ORIGIN      
        1 aaaaaaatac attatgaagg aataaataga gataatagta tgttgtcttc atataaattt
       61 aactttattc cagatataat tcttgtaata tttttaacaa gtatttcttt tacaatatat
      121 gtgaagtcct ttctaggtat tttttttatt ttaggattaa tactactaga tttattggta
      181 tacagtttga tatatgcatt attaaaaatg gtatgtttaa ttaaaaaaat tattaaggga
      241 aaatactcga ttacttttat atatcttaat aatgattgta aaaccaatgc ttttagctct
      301 agtctattaa caataacaat aatatttgtt ttgttgttgt ttatgatatc tgaaataacc
      361 tttgctatgt ttagcaatta tgaagttgat aatagtataa aaaattcaat tgtatatcaa
      421 acaactttag aaggaaaatt aattacagat aaaaaactta tcgaaaatga tatagattat
      481 tttcaatatt atccaattaa aatagatagt atagagtcag taaataatat ttctgttaat
      541 gattatattg aaaaaattcc atatgagaat agaaaagatg taatgaatga tttaaatgac
      601 ttaagtgttg atttattcaa tactaaacag acaatattaa aaagtgattt agagtatgga
      661 aactggttca aagaaaatga taaagaaaat agtataatta taaataatta tcttcataga
      721 tacttaatag attataaact aggcgatact gtaaagttaa gaattaatgg aaagaagata
      781 gactttaaag taataggatt tttaagtcca aaatcattga acaaaggtgt agatttagga
      841 tatataaata taaatgaaaa tatttcacct aaaacccttg atttatctaa aaatcaacca
      901 gtaatatatt tttttaaaga aaagttagat aacactttac ttagtaaatt attacataat
      961 gataaatatg ctaatttaga agactataat gattttgctt attctatgaa aacatatgtt
     1021 gatgatcaaa agtcattact tttgaatatt atatttgctg tgtcattgtc aagtatactt
     1081 ctaatactaa catctcaaat aattattttt ataaggagag tactagatta tcaaattatg
     1141 tatagagtag gaatgagtaa acgaaaattg ataaaaatag gtttattaga aatgataatt
     1201 gtatcaatta tccaaagtac tcttatagca attttttatg aactatttag attcttacta
     1261 atttcattgt tgcagagagc ttataatatt aattatttat tgattttaat agaattttta
     1321 gtagtattgt ctataaattg ttttatattt atattggctc aaagaaatat taaattagac
     1381 ttataaattt tttattgaga caaatctagt atagatttta agtcaaatga agaggttaat
     1441 aattgaattt ataaatcgaa aaacaaccta ttgtgagagc ttaaattact atagaaggtc
     1501 gttttttatt acagaaataa ataagagata gagaatagtt tagaggtgag aaagaaaata
     1561 taatttaaaa catataacaa catataatct tctaatagat aaaggattta atgttgttga
     1621 ttttaaaaat tataataaaa ctattaaaaa tggactctat taaattaata ttaataagaa
     1681 taattattat aactgacaaa aagcaacaca aaagattgat gaagtatatg gaaataaaaa
     1741 gttgacaaaa tatgtctgat gttttatcat attaattgga attaggtagt aattgtttaa
     1801 ggacaagcta agcttattca ttaataatag cagtatccta ggaaggagta tgcccattat
     1861 tatggataag ctttttttat gcttaaaagg aggataatca tgataattaa taagatatta
     1921 aacaataatg ttataatcac attagatgat aatgatgaag aagttatcgt tatgggtaaa
     1981 ggaattggtt atcaaaaatc aaaaggcaat ctaatagata aaactaaagt aaataaagta
     2041 tttagaatat caaataaaga aatatcaaac aaactacaag aattactcaa taatattcct
     2101 atagaacaca tgaaattatc aagtgaaatt atagaatacg cacaaataaa gttaaacaag
     2161 aagctaaatg aaagtattta catatcctta agtgaccata catattctgc aattcaaaga
     2221 atgaaagaag gaataaatgt aaaaaatgca atcctttggg aaattaaaag attttataaa
     2281 gaagagtttg aaattggaat gaaagcacta gatattattg aaaacaaaac aggtataaaa
     2341 ttaccagaag atgaggctgg atttatagcc tttcatatag tgaatgctca gttaagtgaa
     2401 ggtcatactt tggcatctga tattacgaag ttaatacaag aagtacttag catagttagg
     2461 tatcatttta ggatagaatt tcatgaagaa tcagtgtttt attatagatt tataatgcat
     2521 ttaaagtttt ttgcgcaaag attgctttta gatagtgcac atgaaggtga gacagacaaa
     2581 gaattactaa gcattattaa atctaaatat aataaagagt ttgaatgtgt agttaaaatt
     2641 aaaaatttca ttaaaaaaca atataactac atattaacag atgatgaact tatatattta
     2701 acaatacatt tagctaaagt agttaaagac tcaaatatgt aagtgaaata atttttcagc
     2761 agtatattaa gaatgattga aaggagtgat actaacaaaa ttatatttat ggttggatgg
     2821 taacattgag ttggccaaac cttcaaattt caaaaaatta atttaaacga aatatggagg
     2881 agaaaaatgg gaaaatacaa acaattagca caagagatag taaacaatgt tggtggaaaa
     2941 gaaaatatta gtggattagt acactgtatt acaagattac gttttacttt aaaagatgaa
     3001 agtatagcaa atgacaatgt attaaaaaac atggaaggta ttgttactgt aatgaaaagt
     3061 ggaggacaat atcaagtagt tataggaaat catgttgaag ctgtctataa agatgtagtt
     3121 gaaataattg gtttagatga ttcaagtaca agttcagaaa ctaaaaaatc aggtaatata
     3181 ctagataaag gtatagatat aatatcagga atttttcagc cagtgttagg aataatggca
     3241 gcttgtggta tgttgaaagg atttaatgca ttatttgtag caatgggatt atatagtgat
     3301 gtatctggtg gttatatggt aataaatgca gcgggagatg cattatttac attccttcct
     3361 ttgttcttag gatatacttc agctaagaag tttggattaa aaccaatgtt gggattagca
     3421 ctaggagctg cagtatgtta tcctgctata caaggtagca gtatatcagt aggagcagat
     3481 gtgatgtaca ctttatttaa aggaacaatg tttgcatcac cagtatatat tgattttttt
     3541 ggactaccaa ttgtatcaat tgattatact ggaacagtcg taccaattat atttatagtg
     3601 tattttgctt ctaggtgtga aaaattattt aataagtgtg ttccagattt agttaagttt
     3661 ttctttgtgc caatgcttac attattaata acagtgcctg ttgcacttat agttataggt
     3721 cctatagcaa catttggttc tacacttatt tcacaaattg taatttcaat aagagatttt
     3781 agtccactat tggcaggtgc tatagtagga tttacttggc aaattcttgt tatttttggt
     3841 atgcactggg gatttattcc tgtatatata aacaatgtta tgactttagg atatgataat
     3901 gttatgatgc cattctttgc ctgtacattt gcaagttcgg cagttgtgtt ggcaatattt
     3961 ttcaagacta aagataaaaa actaaaagag atggcaatac caaactttat atctggtata
     4021 tttggagtta cagagccagc aatttatgga atattattgc ctttaaagaa accatttata
     4081 ataagttgta ttgctagtgg tattggaggc gctttctatg gattctttaa tcttagaaaa
     4141 tttataactg gaggaatggg aatatttgaa cttccagcaa tgattgaacc agatggggga
     4201 atgggtaact taatagtagc tctttctggt atagctatat caatggtcgt agcttttgta
     4261 ttaactatga ttttatataa agatgagaaa gtagaagagt ctaaaaaagt aagaaataag
     4321 ggtaaagaag aaataaaaga agtcaagtct actaaattag aaagagaaat agtaacaagt
     4381 cctataaaag gtgaagtatt aaaattaagt gatatagagg atgcagcatt tgcatctggt
     4441 gtactaggtc aaggggtggc aataatacct agtgatggaa aagtagttgc tccagtagat
     4501 ggggtagtta caaccttatt tcctagtcta catgctatag ggattctttc tgatacaggt
     4561 gttgaagtgc ttattcatat tggattaaat actattcaat tagagggaag aggttttgag
     4621 gcatgtataa aacaaggtga tagaattaca aaaggtcaaa ctatattaaa ctttgatgtt
     4681 gatgcaataa aagagttggg gtatagtact gtaactccaa ttgttattac aaattcatca
     4741 caatttttag atgtagtaga aacagaaagt aaaaatattg aattagaaga caatctaatt
     4801 acagtcttat tttagtaaag attgaggtat tagcataatc tagtgctttg gttataattt
     4861 taggaggctt tgactatgag ttttaagaat gattttttat ggggaggagc tactgcggcc
     4921 aatcaatgtg aaggtggata caatgaagac gaaagaggat tggcaaatgt agatgtatgt
     4981 ccaacaggaa aagatagaac agcagttatt acagggaaac taaaaatgtt tgattttgat
     5041 gatgagcatt attatccagc taaaactggt attgatatgt atcataatta taaagaagat
     5101 ataaagttat ttgctgaaat gggatttaaa gtatatagaa tgagcattgc atggagtcga
     5161 atatttccaa aaggtgatga agaaacacca aatgaaaagg gacttcaatt ttatgaaaat
     5221 atatttaagg agtgtaaaaa atataaaata gagcctttag tcactattac acactttgat
     5281 tgtccaatgc atttagttaa aaagtatggt gcatggcgta atcgaaaaat gataggtttc
     5341 tatgagaaat tatgcaatgt tatctttaga agatataaag gattagtgaa gtactggcta
     5401 actttcaatg aaataaatat gattttacat gctccattta tgggtgctgg tatttgcttt
     5461 gaagaaggtg aagatgttga aaagataaaa tatcaagctg cccatcatga gcttgtagct
     5521 agtgcaattg caactaaaat agcacatgaa gttgattcaa ataacttaat tggatgtatg
     5581 tttgcagctg gaagtgtata tccttatagt tgtaatccta atgatgtatg ggaagctaca
     5641 aagttagata gagaaaatta cttctttgtg gatgtacaat ctaaaggtaa atatcctaac
     5701 tatgcgttaa agtatatgga gcaaaaaggt attacaccag aaatggaacc tggtgatatt
     5761 gaattattga ataaatatac agtagatttt atttcatttt catattacaa tagccgttgt
     5821 gttagaaccg atgaaaatgc agatgatatg gctgaaggaa acatatttat atctgctaaa
     5881 aatccatatc tttcatacag tcaatgggga tggccaattg acccattagg tttgagaatt
     5941 acactaaatc atgtatatga tagatatgaa aagccactat ttattgtaga aaatggatta
     6001 ggtgctaaag atatagctga tgaaaatggt tatgtagaag atgattacag aattgattat
     6061 ataagagaac atataaaagc tatgcatgat gcagtttcta tagatggggt cgatttatta
     6121 ggttatacta catgggcacc tattgattta gtaagtgctg gtacaggtga aatggaaaag
     6181 cgttatggat ttatatatgt agatagagac aatagtggta atggaagtct aagaagaatg
     6241 aaaaagaaaa gttttgaatg gtataagaaa gtaattgctt caaatggtga agatttatat
     6301 tagacaagaa atctatgtta aataagaaat ttgtattaaa taagaagagt ataaaaatta
     6361 ataataagta taatttttaa cttttttaac tatagaaaat caatatagaa ttattttaca
     6421 ttgtattgat gcaatagtat gattcacaaa taaaatttta gaaaataaaa ttgttaagaa
     6481 gtatgggaag taagtgcaat ttactttgta ataaaagatg aaaaaggcaa gcttaggttt
     6541 taagcttgtc ttttttatta tgtataaact atgatatttt aaaattctat gaaaaattta
     6601 tttaatagag aaattaaaaa tttaatggta atcaaattct ttaccaatga atataaaaaa
     6661 tgtctacgtg ttataatagt ttaaactaat aaacgaaagg tagatgaatg aatatgaaat
     6721 accaacatat aatagaagtt gacaaagaac tttgcattgg atgtgggctt tgtaaaaatg
     6781 attgtccagt aaacaatata attatagaaa ataaaaagtc agtaattaaa aaacaagatt
     6841 gtctaatgtg tggacattgt gcagcaatat gtcccacaaa agctattgcc ttgacaggat
     6901 ttgatgaacc tcctatagaa cttacaaata agccaaagct agattcagat gaattactaa
     6961 tggcaattaa atctagaaga agtatacgta aatttaaaga taaagaagtt tcttcagaaa
     7021 ttgtaaagca aattattgag gcaggtaggt atacccctag tgctaaaaat tcacaagatg
     7081 tatcatatat tgttttagac aataaaaagt caatttatga aaacgaagca gtgaaatttt
     7141 ttagaaaaat aaaacctatt gcaaatatag ctattaaata ttcaaaagaa gttgagattg
     7201 acgataactt tttctttaag catgctccta ttgcaataat gataattaca aaagataaga
     7261 ttagtggttc acttgcagca tcaaatatgg cattaatggc agagtcttat ggcctaggtg
     7321 ttttatttag tggatatttt tcagatgtag caaataattc gccaaagtta aaaaagcttt
     7381 taggtctaaa acgcagtaat catgttctta caacattagt aattggatac ccagatgtta
     7441 aatatagacg tactgcacag aaagaagtag caactgttag atacttataa gagagggtgg
     7501 attttatgga aaaagaatgt caacaaagaa tgcaagaaat aacgtctaaa tttaaagaat
     7561 gtcgcaaagc tatctctgct atgggtgatg agactagaca attgatttta attgctctct
     7621 tagagagtga ttttaatggt atacgagtgg gagaaatcac aaaaaaaaca catctttctc
     7681 gtcctgctgt atctcatcat cttaagatat taaaagaagc agagattgtt aatttgcgta
     7741 gagaaggaac gaaaaattat tattatcttg attcaaaaaa atcacaatgg aagtcactaa
     7801 cagagctaat aaatcttata tttgttggaa tacaacatat tagtgaaaat gatataagaa
     7861 aaggtgatta aattatactt gatttaatac aaaaaagatt gatttaggaa ctaaaaagtt
     7921 cactttgaga cactatcttt tgaactatac ccatcttagg agacagttaa aatatgtaaa
     7981 actgtataaa acaatgggta tagttcattt tataatggtt taaatattaa gaatatatta
     8041 ttctaaatag tatctttctc tgttttgagt tttattttta tactcaatag cttcagtggg
     8101 gcaaccacaa atacatgcca tacaatgagt acagttattt ttccaaactg gttttttgtt
     8161 ttttaaatta atattattta aaggacatag ctctacacat tttccacagc caatgcattt
     8221 atcagtataa tgaaatcctt tggcactaat aattgtctta taaaaaactg tatttattat
     8281 accactttta attgtacctc ctaagccact aggagtaata gtttcaaatt cattttcatt
     8341 gcgtatatca gaaataatcc tatatatttg tttatctgct tctttaatca tttgttttga
     8401 tgtttctttg tctgttgtag aaaataaagc tatataattc tctggcattt taatttctgc
     8461 catacctttt aattgccatt ccttatattt acatagtttt tgaatatagt ttatggattt
     8521 tgctgtatct ccaccacaag tcattacaaa gtaaactctg ttgcttccta gaaattttgt
     8581 ttcttttata aaatccgtaa ccactattgg caatctccat gcataagtag gacatacaaa
     8641 tatgaatggt ttatctgctg atattaattc atctgttttt tcctctttta taagttgatt
     8701 tagtgatatc agttcatcat ttaattcttg tgcaatttta cgagccacat atcggctatt
     8761 accagttccg ctaaaatata aaatcataat aatatctccc taactatttt ttattttaac
     8821 tcttaatttt ttatttatta tagtaattta ttgatatatt ctattcaaat gtatgtaaaa
     8881 tttagtagtt aagaaaatta taacacatta gtaattttta tacttattgg taaagaattt
     8941 gattaccatt aaatttagca acaagacatc aactggactt gtatatgaga agggataata
     9001 atttgcttat gaaagattat aaatataaag gttattttaa aggtgtagaa atatttgata
     9061 atgtaaatga ccttaactta aatactttaa caacagttag tagatttgtg cttgtttttt
     9121 tgtttacgat aacttaagga aatatgttac ctgaataata aatttatata ataaaagtat
     9181 ataaatatat atatgaatgt taagattagt tgatagaatt taaataataa atccaatatt
     9241 ataaacatag gaggtatgaa aaatatgatt aatgagaatt taaaaagtct aagaaaaatc
     9301 aaccagtata cacaagaaga attagctgaa aaacttaatg tttctagaca atcaatagca
     9361 aaatgggaaa gtggagaatc tattcctgat ataggtagtt gcattaaact tgcaaaattg
     9421 tacaatgtca aactagatga cttagttaat cattcagaag aaaaaacagg tattatagta
     9481 cctcctaaag gaaagttttt ctttggagca gttgtagttg gtgaacgtgg tcagattgtc
     9541 attccaaaag aagcaagaga agtatttaat ataaatgcag gagataaatt attggtactt
     9601 ggagatgagg aaagaggatt aggaattgtt catcaacgtg atttgataaa ttttataggt
     9661 catgtgggag tagtacgtaa agaagaatga tttaaaaaaa ttataaaagg aggtatgaaa
     9721 tatgagtaaa atagtatttt ttagcattcc tgcatatggt catactaatc ctactattga
     9781 agtagttcgt gaattagtgg atagaggaaa tgaggtattg tattattctt ttaatgaatt
     9841 taaagataaa attgaaggtg ctggagcaaa atttatttgt tgtgataaat accttccaga
     9901 attactccct ggagatgaga aaaaaattgg taaggatttt cctggattaa ttgagatgat
     9961 agtagatact actatttctt tagatgaaaa agtttgtaga gaacttaaag attttaatcc
    10021 agattgcatt gtatcagatt cattaagttt ttggggaaag ttatttgcac aaaaacttaa
    10081 tattccatat gtatgctcta caacaacttt tgcatttaat aaacatacag caaagttgat
    10141 gaaacaaaac tttatagaaa ttatacgtat gatttttggt gtaagacgca ttaataaaaa
    10201 aataaagctt ttacaaagaa aagggtatga agtaaaaaat tttatttcta tagtttctaa
    10261 tgataatgat acggatacca ttgtatatac atcaaaagaa tttcaaccaa tggtagaaac
    10321 cttctctagt aagtattcat ttgtaggtcc atcagtatca aagttgatta ttgaacctaa
    10381 agagagaaaa agaaagctaa tatatatttc gcttggtaca attaataata aaaatgttag
    10441 cttttataaa aactgtataa atgcatttaa agattctgat gttgatgtga ttatgtctgt
    10501 tggtagaagt actaatatta aaagtttagg aaatatacca aataattttg aggttaaaaa
    10561 tagtgttgaa cagattgcta tacttcaaaa aactgatgtg tttgttactc attctgggat
    10621 gaatagtgtt aatgaaagtt tatattatgg agtacctatg gtattatttc ctcaacattc
    10681 ggagcagaga atggtagcta agagagttgt tcatttggga gcaggaatta tgttaaaaga
    10741 ggataagtca gaaagtatta aaaaagcagt atttcaagtt ataaatgaca atgaatataa
    10801 agaaaatgca actaaactat caaaaagctt ttataatgca ggaggctcaa aaaaagcagc
    10861 agatgtaata ttacagatta tatgtaatag tagacaagtt gacatataat agatttgtta
    10921 aaaatatata tacatacatg taatttaatc aaagtactga cagtggggtc ttttaaccta
    10981 ccagttgggg catatatatt gatgctaaat atgagggatg ttatatttta ataaaaaaga
    11041 taacatcctt tttttagtgc attatatcga gttttaaata ataaatgaag gaggattaat
    11101 atgaaaaaat ataaagttat ttttatagta ttaattatag gtattataag tgtaacaggg
    11161 tttgttgtat ataaaaacta cattaaacca tggaaagatg ctgaaacata tattgataag
    11221 tatatggtaa agcaagatat ttcaaaagat aatataaaaa gtattacaaa agaaaaagcc
    11281 aagaaggcaa gttacgaagg aatactatac aaagtgtatt acaaaggtga ccctcgatat
    11341 gagtatcaat atttttatag tgataattat tatgctctat atgtaaataa agtaatgtta
    11401 caaatttatg atatggaaaa taataataaa cttttaagaa gtaatgagga attaaaaagt
    11461 gtaaagtatc caccaatata tcttaaatat aagccagata aatgatttat gacaaaatag
    11521 aatgattttt attaaaattt atttaaaaa
//
LOCUS       DAENCV010000116        11440 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAENCV010000116 DAENCV010000000
VERSION     DAENCV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.117"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..623)
                     /locus_tag="KQD82_003023"
     CDS             complement(<1..623)
                     /locus_tag="KQD82_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG4381479.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGIERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIY"
     gene            complement(623..1486)
                     /locus_tag="KQD82_003024"
     CDS             complement(623..1486)
                     /locus_tag="KQD82_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381480.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            complement(1483..1857)
                     /locus_tag="KQD82_003025"
     CDS             complement(1483..1857)
                     /locus_tag="KQD82_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4381481.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            2224..2628
                     /locus_tag="KQD82_003026"
     CDS             2224..2628
                     /locus_tag="KQD82_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG4381482.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     KDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIVYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            2645..3241
                     /locus_tag="KQD82_003027"
     CDS             2645..3241
                     /locus_tag="KQD82_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381483.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            3278..5872
                     /gene="clpB"
                     /locus_tag="KQD82_003028"
     CDS             3278..5872
                     /gene="clpB"
                     /locus_tag="KQD82_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG4381484.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6068..7495)
                     /locus_tag="KQD82_003029"
     CDS             complement(6068..7495)
                     /locus_tag="KQD82_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4381485.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRSFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            7637..8533
                     /locus_tag="KQD82_003030"
     CDS             7637..8533
                     /locus_tag="KQD82_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4381486.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            9198..9338
                     /locus_tag="KQD82_003031"
     CDS             9198..9338
                     /locus_tag="KQD82_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381487.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(9335..9706)
                     /locus_tag="KQD82_003032"
     CDS             complement(9335..9706)
                     /locus_tag="KQD82_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381488.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            10255..10512
                     /locus_tag="KQD82_003033"
     CDS             10255..10512
                     /locus_tag="KQD82_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381489.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(10622..11302)
                     /locus_tag="KQD82_003034"
     CDS             complement(10622..11302)
                     /locus_tag="KQD82_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG4381490.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT         4630 a         1298 c         1596 g         3916 t
ORIGIN      
        1 ctatagattt ttgatgatat aaaatatgaa atcacaaata gtaaaatagc aacaaatgga
       61 agagaatata ctagtgtatt aagtataatt tctatagaaa taggtgaact aatattttct
      121 aaaatacttt taaaaactaa taaagctagt gttggaacta aaaagcataa aatcattaat
      181 aaacgagctc tttctatacc aaatttataa ataagtggta gaataaggct accaaaacat
      241 actgaaattg ataagattag tgcattaata agtagtgttt catttaatat aagtgtattt
      301 ttaaaataac caatagttac actaacaata agtgaaacaa gaacacctat aaagctaaat
      361 attaacatag ttaaatattt acttaataca atatcattgc gattaatagg cattgtaagt
      421 acataactat cccatttatt caaatcatca tatgaaaatg ttgaaacaat cattgtagta
      481 cataaaacta taataacacc tataaagtta ttaacatttc catctcctaa agaagacata
      541 aagccaaaca taatgatgaa taaaagtata aactttatat ttcctttcaa attaagtaaa
      601 tcttttaaaa ttagaccttt cattatttat cacctctcac ataaaataac ataatttctt
      661 ctatagacgt attatcaaca atacagtttg gatgtcttga gataaatcta tgtttatctt
      721 taagaagtac ttcatatcca aattggcttt tacgataata tgaataatcc tctcttgaaa
      781 ggttatcaaa atcactaggt ttacatttta aaactcccat agtatcaatt aattcatcct
      841 tattttcact aaatacaatt tttcctttat gtataaatgt aatatagtct gctatcttat
      901 ctaaatcact tgtaatatgt gaagataaga taatagagtg ttcttcatct tgtataaaat
      961 ctaaaaatat atctagtatt tcttcacgaa cgatagggtc aagtgaacta gttgcctcat
     1021 ctagtattaa taattttggt ttatgagata aggctacagc aatactaagt ttcatcttat
     1081 tcccctttga aaactcctta attaatttat tgtctggtac tctcaatttt tttaagtagt
     1141 ctttaaataa ggtgtcattc caatttttat agattttact catgattttt gaaacatggt
     1201 cagtgtttaa attttcatga aagttgcttc cctcaaatac aaccccaatt tgctctttaa
     1261 tttctttttc atttttttta ctatcaagac caaagatttc tatatttcca ctatcataag
     1321 atattaaatt taaaattgct tttaatgtag ttgtttttcc tgcaccattt tcacctacaa
     1381 atcccattac actccctttt ggtactgaaa aagatatatc ttgtaggcta aaatctttat
     1441 aagttttatt taaatttttt atagttattg catccatact tatcattctc ctttatataa
     1501 taaaaataaa atatctttta attcgtcata tgtaatatca ctactttttg ctgtatcaac
     1561 tgctttttgt aggtattctt caattaaacg aaattgttct tctttgacaa aatccatgtt
     1621 tttactagat acaaaactgc ctttaccagt aactgtctca ataaatccgt ctcgttctaa
     1681 atcgctatat gctcgttttg tagtaattac acttattcta agttcttttg ccaatagtct
     1741 catacttgga agagctgagc cttctggtaa ctctccactt ataatcatac ttttaatttg
     1801 tgtagtaatc tgttcatata ttggcttccc actcgaatta ctaataacta tattcaataa
     1861 gacacctcct taaattgtat atatagtata tacacattat ataaatatat atataatatg
     1921 tcaactgatt ttagaaaaaa ctaattgcca atattataca tttaataaat gtaatgtatt
     1981 ttatgaaagt ctgccatatt aaatagattt aaattttttt aaaaaattta taaatattgt
     2041 tcaaaaatcc tgccaataaa ttggaaatac taaattaaaa aaataataaa gtttagctta
     2101 tagctaaata aaagtttgct tataaggtga actttttgca tttttagttt tgacatacat
     2161 atttttttgt aatatattta tattatcaat tagagataag gagagtgaga agagatattt
     2221 aatttggata agacattaaa aaaggattta aatcaggaat atacagagag caaaagttta
     2281 gttatgaata aaagtaagta tatgaatgtt tgttttaaga attatattaa agtagtagaa
     2341 ggaatgtata ctaagaagga tttaattcca ttaaaattaa taaactctat ggataaaaat
     2401 aataagatta gaaaaacatt atatgaatta tcttgtgaat ttgaatatcc aaaaacttct
     2461 ttatcttcat tgtttacgga gctgaaaaaa atggattttt tacaaagagt gaaaaatggt
     2521 gagtatatga taaatccaca tatagtatat aaaggtagta gagctgataa agaaagttta
     2581 ttagcagaat ataatcagat aaaaagacct aataaggtta gtaaataagg aagaaggttt
     2641 ttatatgagt ttaaaacttg atagaaatgt tctacagtgg tttgactatg tatttgagaa
     2701 tgaaaaaact agtttgagac actataattt taattgtaca ctaaaggaga ttagttctac
     2761 cagtttgaac aaagtagctt ttattttaga aaaaaataac tctaagtact ggaagcttta
     2821 ttttgaaata ccagctgaag ttactttaaa acttaagcaa aacattcatc ctttatttag
     2881 ggaatatata tacgaacaaa tatcactgta taacaataat caaatttaca attttgtaaa
     2941 ttcaaacata ttaaaagtgt ttaataatat tgctatttat caatataata ttttagaaaa
     3001 tctatacact attgatttta aaaagagttt tatagataaa tgtcagtatt tattaatagg
     3061 tgaaaaaaga cttatagatg aagacttgta tttaatagca aaatcaaaag aagtattcga
     3121 tttttttaat tcagatggaa catttaattt aacattgagt ttcgatatac aaaaaaacga
     3181 gaatttactt gatagtcttt tagagcttag aaaatcaata attataaatg agagaattta
     3241 atttatttgg atataatatt aaaatggagg gatttatatg gacgtagaaa aaatgacttt
     3301 aagagttcaa aaaagtttaa atgaggctta taacgaagca gtaaaaaatc ataaccaaca
     3361 agtagatgta attcatcttt tctcagcact tattaatcaa gaggatggat tgataccaaa
     3421 tataatcgaa aaaatgaata tatcaattga ttctttgaga aatactgtaa actttgaaat
     3481 tgataagctt ccaaaagtat atggagaagg tgctgactca caaggagtat cagctactag
     3541 aaaaataaat gaggtgctta taaaggcaga gagtatttct aaagaattta aagattcata
     3601 tataagtgta gagcatgtta tgcttgcaat gatggaaaca gaatcaaaat cagctgttgg
     3661 taaaatatta aaacaatata atataaataa gaatgacttt ttaaatattt tatcacaagt
     3721 gagaggtagt caaagagtag aaacacaaga cccagaaggc acatatgatg cacttgctag
     3781 atatggaaca aatttagtag atttagccaa gaaaaataaa ttagaccctg taattgggag
     3841 agatgaagaa ataagaagga ttataagaat tttatcaaga agaacaaaaa ataatcctgt
     3901 attgattggt gagccaggtg taggtaaaac tgcaattgta gaagggttag cagagagaat
     3961 agtaagagga gatgtcccag aaggtttaaa agataagatt gtattctcac ttgatatggg
     4021 agctttaatt gctggtgcaa aatatagagg tgaatttgaa gaaagattaa aggcagtttt
     4081 aaaagaagtt caaagttctg atggaaaaat tatactgttt atagatgaga ttcatactat
     4141 tgttggagct ggtaaaacag aaggttcaat ggatgcaggt aatcttatta aacctatgct
     4201 tgcaagaggt gaattgaact gtattggtgc aactactttt gatgaatata gaaagtatat
     4261 agaaaaagat aaggctcttg aaagacgttt tcagcctgta attgcagaag aacctactgt
     4321 tgaagataca atatctatac ttagaggttt aaaagaaaga tttgagatac atcatggagt
     4381 aagaatacat gataatgcta ttgtagcagc agcaaaactt tctcatagat atatacaaga
     4441 tagattttta ccagataaag caattgattt aattgatgaa gctggagcta tgattcgtag
     4501 tgaaatagat tcattgccaa cagaattaga tgttgttaga agaaagttat ttacattaga
     4561 aactgaaaga gaagcattac ttaaagaaaa tgatgataaa agtaaatcaa gattggatga
     4621 tattcaaaaa gaaatagcag agttaaaatc taaaaatgat gaaatgacag caaaatatga
     4681 aaaagaaaag agtcaaatat tagatattaa aaacttaaag gctcaattag acgaagctaa
     4741 aggtaaagct gaaaagtatg aaagagaata tgatttcaac aaagcagcag aggttaaata
     4801 tggtgaaata cctaagttag aagaacaaat aaagcagtac gaagaaaata tgaatgatgg
     4861 ttctgaaaat tctcttttaa aagaagaagt tactgaagaa gaaatatcta gcattgtatc
     4921 aaaatggaca ggtataccag taactaaact agttgaagga gaaagagaaa aattacttaa
     4981 actagaagat gaacttcata aaagagttat tggacaagat gaagcagtta cagcagtttc
     5041 aaatgcagta atacgtgcaa gagcaggatt aaaagatgaa agaaaaccaa ttggttcatt
     5101 tattttctta ggccctacag gtgttggtaa aactgaactt gcaaaaactc ttgctagaaa
     5161 tttattcgat agtgaagata atataataag aatagatatg tctgaataca tggaaaaaca
     5221 tgctgtatca agactagttg gacctcctcc agggtacgtg ggatatgaag agggtggtca
     5281 acttacagaa gcagttagac gtgctcctta ttctgtaata ctatttgatg agatagaaaa
     5341 agcacatgag gatgtattta acatgttctt acagatatta gatgatggta gacttactga
     5401 taataaaggt aagacagtag actttaaaaa tactcttata ataatgactt ctaatatagg
     5461 aagtaattat ctattagaag caggaggaaa tataacagag actactaaca atttagtaat
     5521 gaatgaaatg aagcatagat ttaaacctga gtttttaaac agagtagatg atattataat
     5581 gtttaagcct ttagaccaag aaaatatcaa gaaaattata gatatattta tgaaagattt
     5641 aaaaaataga ttaaaagaaa aagatattac tattgaagtt acaaattcag caaaagatgt
     5701 tatggtgaga gaaggatatg accctgtata tggagcaaga cctcttaaga gatatatagg
     5761 aaatacatta gaaactataa ttgcaaagaa acttattgct ggagatgtgt ataatggatg
     5821 tactatagtt atagatggaa aagatgaaaa tattgaagtt ttagttaaat aataaaggaa
     5881 tcagttgagc ataatatact aaatattttg ctcagctgat ttttatttat aaactatagg
     5941 aaaagaaata ttttatcaaa ttaataaaac atttttaagt caatatgtcc aaattaaatt
     6001 taactcaaag gaagtatttt agtaaatttc ttataatata atcataattt gaatataatt
     6061 ataaaaacta ttgttcattt ttattatatt taaataaata aaagccaatt aatattgcac
     6121 ctattaagca aacaagtgta acaataatat aaacatttct cattccaaat acaaatattt
     6181 tatcattatt aatcacataa tcattgaccc tatatccagc tttaatactc ataaaattat
     6241 ataaaagtgt tgtagaaata gtaataccta taacttgtcc taaatttctt acaagggaat
     6301 ttatgcttcc tacaactcct agcttacttc tagaacagct tgacattatc aaagcattgt
     6361 tagcaggttg aaatatagct tgtcctattg atattatcga tatataaatt acaaataaaa
     6421 taagggcaga actttctttc aatcttgaca ttagaaagaa tccaaaactc ataacgagaa
     6481 gaccaattaa aataattttt tctgacttaa ttttatctga tatattccca aatataggtg
     6541 aaaaaatggc taaaatcaaa ggtgatagta tcataaataa acctgcttga atagggggaa
     6601 gtttcatagt actttgtaaa taaaatggaa ttaaaatact tgatgatgca atacaaatga
     6661 atgatgtaag agcacaaatt aaattcaatg aaaatttaaa acttctaaaa atttcaaggt
     6721 caagaagcgg ttgaggatgt tttttttcta atatcaaaaa taatataata aaaataattg
     6781 ataataatag tgcaattaat atatatggat ttagtaatcc tgtttgttga gcactaatta
     6841 gagcactaaa aaataatgtt gttcctaaaa attgaagtat agcaccagtt ttatccatat
     6901 tttctgaaga ttttttacta ttaggaagaa attttgtatt taaaaagatt acaataagac
     6961 cgattggaat atttataaaa aatataacat tccaatcaaa tattgacaaa ataaatcctc
     7021 ccacagatgg tccaaccata tttccaatag caactgcagt aactaaaatc cctagggctt
     7081 ttcctctgct ttcttttgga aatagttctg tgataattcc atgattgttt gccatgtagg
     7141 cagaagcgcc tattccttga ataaagcggc aaactataag taaagttaaa gaattactta
     7201 atccacatag taatgaagct gatgtaaaga gaatagtacc tacttgaaat actcttgatt
     7261 taccaataat atctccaagt cttccaaaaa ataatagtgt tgaacaaata ataattgagt
     7321 aacttgctat tacccattca atagatgcta gagatacatt tagtttgctt gcaagaactg
     7381 ggagagcaac atttataata ctagaatcta atgttgccat aaaagtcatg gcaatgacta
     7441 caaataaaac tgtgattttt tttctattat catctttttt aaaagagttt gtcattttta
     7501 ttacctcctt attataatga ctggcattat gttaatgttt acaatgatag ttacatttta
     7561 tataatagcg atatgaaata atgaatacaa taactatatt gcttataatt agcactattt
     7621 tgatgaaggg ggaataatga aggataaaaa tacatatttt aacaggaaag gtcaagaaaa
     7681 ctttatagat atttttttaa aggatgacat taaaaaagga gcaaacttta aaagacattg
     7741 gcatgagcat ctacaaatat attattttat tgatggtata gcatatcttg aatgtggatt
     7801 ggataatttt aaggtagtgc ctggaaatat tgtaatagca aacagtaatg aattacatta
     7861 tttagaaagc ttatcagata atttaaaatt ctatagtatt cgtattgaac ccacattcct
     7921 ttttagtaat caagttgatt tgttacaaac caagtactta gcaccattgg ctttaaatca
     7981 tattgtattt aataatttaa ttcaaaatga ttctctgatt ttaaaatgta ttacaactat
     8041 acttgatgaa tatcacaata aagaagttgg atatgagtta tctataaaag ctaatatcta
     8101 tcaattaata gtactcttat taagaagtca tattgataaa atatattctg aagatgaatt
     8161 tataaaaaga aagtattctt tggaaaaatt tgaatgtatc tttgatttga tagaagaaaa
     8221 atatatggat aaaattacat taaaagaact tgctagtcgt gtgaacttga gtgcacatca
     8281 cttttgtaga acatttaagc aaataacagg taagactaca atcgatttta taaatggaat
     8341 caggcttgat aaagctatat attatttaaa ggaaacagat ttaaatatga cagaaattgc
     8401 tatgagatgt ggctttgata gcataaatta ttttagtcgc ttatttaaaa ggaattataa
     8461 tatatctcca actaagtata gaaatgaaca tagagaagtt agtaatgtta ttttaaaaga
     8521 agatatgaaa taattcataa atatacttta tagaaaatat atatttttaa atctttgagt
     8581 ttttcttaat aaatattaat aaagtatagt acataaatga gtaagactac ttgtatagtt
     8641 aaatgtgaaa atgagtatga aagaatgcag tgtctatgaa aagtatattt taatatgaaa
     8701 ctaaaactgg aaaaataaga atagcagaaa aaatgattat attataaatc aatttttttg
     8761 agacatctgg cttagattct gaaagatatt taatcaaaga aataaaacta ttaaataagc
     8821 aagtaaacag ctaaaatagt atgattttta tagaaaattt aaatccatcc taaagaatta
     8881 aaagaaagtc aaataaatat aaaaaaatta tatatttata aaaaatttta taataaaaat
     8941 catcctgaaa aatatataaa aatagaaaaa acatacaaga ttctacaata atagattttt
     9001 ttgttattat tacgagtttt ttattgcaac tgtagaataa aagtattatt atataattaa
     9061 gataattatc tagggaaatt ttggataatc aaaatatatt ttataaaatc aaaatacaat
     9121 tacataaact ttattaaaaa tttgttggga atttctgact aacattaaga acgtaagttt
     9181 gttgggggat ggtatatgtg gaaaagtcaa acactactgt tacaaaaaaa gaaaaaattg
     9241 aagatatagg aatgaaatta gaacatttat tgagtgaaga tattactttt tatgaattat
     9301 tacttgagat tttgtataaa gaaaaagaag agaattaatt ctcttcttta aggaatcttt
     9361 ttaaataggc attatcaatc attttttcta tagcttctct atcttctttt tcaagagaat
     9421 atattttttc taagagcttt tttgtttttt cacttactga taagttctct atgattaatt
     9481 tatcttcttt gcttattttt gtattatcat ttacgcctaa taaataatct gttgatgttt
     9541 ctaatgcttt tgctaatcta actattattt cacttttagg ttctcttaaa ttattttcat
     9601 aacgtgacaa gctagcctca gttataccga ctttagtagc aagttctttt tgatttatat
     9661 ctaattcttt tcgtagattt gctattctat ctcctaaact ttccatatgt acatcactcc
     9721 ttaccgaaat tataagttat tttgataaaa agtaaaataa aattaccaaa aatataaaaa
     9781 aaaaaggtta aaataataaa atatattgaa aattaccatt tgtatatgta taatatgaat
     9841 tatacaaatg gtaatttgat gagaggtggt atgttgaaat aagaaactga attaattaat
     9901 aaatatatag aaagttttga aaacccttaa ttgagatata aacaatatgt acaaaatttt
     9961 ttgcaagtaa aattaccaaa tgtataaaat aaatcagtag catagataat tttatgcttt
    10021 aatacattag attatctaat ttagttataa tggtcaaaaa tgcaatctac aaataaaaca
    10081 aagtattatc aaaaaagtat tctttaagag aattttttat tagagatgta attgatataa
    10141 ataatcgtat taaaaaataa aatactaagt ttaaaaattt aaataatcta taaatcaatt
    10201 ataaaatatt atttcttaga aattaaaatg aattaagtaa atggaggaat tgatatgata
    10261 ataattagaa gtcaagataa aactaattta atgcagataa accaaattaa aatagatggt
    10321 agtcaagttt atgcagtatt tgaaagtaaa attgatactg taaaaatagg agattatgaa
    10381 aataatacaa gagctataca agtgttggac aatatacaaa acttcattga aaatggaaca
    10441 aaatatgatt atataacatc aaataaagtc agatacaatg ttaataaaat atttcagatg
    10501 ccagctaaat agtgagtgtg cattttaatt taataactaa agcccacttt cttttgtagt
    10561 atgaaagagg gctttacata tttgtaaata tcgaagtaca cttgttttat caataaatta
    10621 tttaataagg tataatggac caattaaaca ataaaaattc tcctaaaaca gcaagtatta
    10681 aatatataat agcttcattt ttgtaaagag aggtaatttt ttcgcttatg tcatcatcaa
    10741 ttgtaatagc aatttcatct tgaataatat ttttatatat agatgggaaa atatcttttt
    10801 tctttataag attaaataaa ttatttacac ctacccatat caaatatcct aaaacagcac
    10861 ctaatgtgtt catcattaaa tcatcaatat ctgtagctct taagttaaac aattgacaaa
    10921 gttcaattgc taatgaaaaa cctaatccag ttaatgaaac attgataata tttctaaaat
    10981 ttttccaaat taaaggtagt aaaaatccta aaggcataaa cataattata tttaaaatat
    11041 atgttaagat gccaactgag tcaaaaggta ttagatttat ttcatcaatt ctaaaaagcc
    11101 cactatacct tcctatatcc catatgctac caattccagc tttactaagt gctagataga
    11161 tatataataa aaaaatatat acccatacta aatgtatcat tacattcttt tcaactttca
    11221 aattttttcg ttttataacg actatttggt atattaagca tggtaatata acgcaaataa
    11281 atgaataaaa tgtccatatc attttatttt gtcccctttc aaatttatgt ttttagtttt
    11341 ataagtacta tctttatatt aaactgtaag ttttaaaaat cttttaataa aatcttaaaa
    11401 atttattaat ttataaaaat atattattaa gattaaaaat
//
LOCUS       DAENCV010000117        11263 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAENCV010000117 DAENCV010000000
VERSION     DAENCV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.118"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(239..2326)
                     /locus_tag="KQD82_003035"
     CDS             complement(239..2326)
                     /locus_tag="KQD82_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBG4381491.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENTDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNADILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYIVIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            2687..3265
                     /locus_tag="KQD82_003036"
     CDS             2687..3265
                     /locus_tag="KQD82_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBG4381492.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            3258..3917
                     /locus_tag="KQD82_003037"
     CDS             3258..3917
                     /locus_tag="KQD82_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBG4381493.1"
                     /translation="MNKQKFHKINLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(4128..6002)
                     /locus_tag="KQD82_003038"
     CDS             complement(4128..6002)
                     /locus_tag="KQD82_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4381494.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMIKDRETINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(6007..7671)
                     /locus_tag="KQD82_003039"
     CDS             complement(6007..7671)
                     /locus_tag="KQD82_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG4381495.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(7685..8506)
                     /locus_tag="KQD82_003040"
     CDS             complement(7685..8506)
                     /locus_tag="KQD82_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4381496.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(8605..9369)
                     /locus_tag="KQD82_003041"
     CDS             complement(8605..9369)
                     /locus_tag="KQD82_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4381497.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(9366..10115)
                     /locus_tag="KQD82_003042"
     CDS             complement(9366..10115)
                     /locus_tag="KQD82_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4381498.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(10619..11224)
                     /locus_tag="KQD82_003043"
     CDS             complement(10619..11224)
                     /locus_tag="KQD82_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4381499.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         3837 a         1978 c         1195 g         4253 t
ORIGIN      
        1 ataatataat accctataca aatagtggca actctccctc acatcctcaa ttgttgccac
       61 tatttaattt tttgtatgaa gttaaatata ttttcatcat ttcaacaata aaaaagaggt
      121 ctatctctat tttgaaacaa cctctttaat tattttagag tttactaaga agtctaaaaa
      181 caatttatct aaaatattat tctcaactga gtaagattaa tttccaaatt gagtaaattt
      241 aatcttcaaa cactgattta acaataggaa taacataatg acttccccca cgactcttta
      301 catcttcaat catagcaatt actatatatt gtttatcact attttcatct acagtaaaag
      361 cattaaacca tccaagttct gtccctgtaa catcatcttt tgatgcttta atttctgctg
      421 taccagtttt cccagcaatg ctaattcctt taatcatagc actatgacct gtaccatcag
      481 ggctttcaac aacttgaagt aagtcatcac gaataatttt tacagcctct ttagaaaaca
      541 cacctttctt ccaaaattca gcctttttaa attcaagatg gggttttatc atgtctccat
      601 catttacaaa agctgaatat attgatgccg tatgtactgg atttacaaga agcttacctt
      661 gtccatagcc agtatctgct aattgtattt cagtctcaaa tttattgtca gtgccaaatt
      721 ttgaagaaga caatccaaat tcaaatggca tactttcatc aaatccaagc ttcagcaact
      781 cttttgctaa tacatcttca cctattttta aggctgcctt agcaaaataa atattgtcag
      841 aataaatcaa agcatttttc atatttgcag tatttccata atcttttaac gtggttacct
      901 tataacttcc ccaactagaa tctttttgcc aacttagact gctgtgtcca aagttttcat
      961 ttggattaat ctttcctgtt gttataccaa taccagcagt aactggttta aaagaagacc
     1021 caggacataa tcgtgcttta aatctattgt acattggttt attttcattt ttattgagtt
     1081 cattccatct atcttctgac attcccaata taaactcatt tggattgtac gacggactac
     1141 ttacaagtga taaaacctct cctgttttag gattcataac tactgcacaa cctttatcat
     1201 ttttcatttg gtcatatagt ttactctgta gttttgaatc tatagttagc tttacatctt
     1261 ctccatctac ctttggctca gttactaaag tttctttctt gtcaccatat ctatcagcta
     1321 ttataatctc atatccatca ataccacgta ttctatcttc cagtaaattt tctaaaccaa
     1381 tcttaccaat tatactatta tcgttgtacc tcttaccctc tttttccttt agtatatctg
     1441 catttacatt ttgaacataa ccagttaaat gaccggtttt ttcaccaaat ggatatactc
     1501 taacctctgt attagatatt ttaactccag gaatactaag aagtgactct tgtagttcct
     1561 tatcttcttc ttttacagta ccatctgtat tttcttttat tttttcaata gtttttatag
     1621 gtaccattga attttctttt acccaagatg catttaattt cttttcaatt tgttcaactg
     1681 atacattcaa taaagtagat aattttttta agtcttctat attatcactc atttttccag
     1741 gaactaatcc tatcatagat gcaagacctt gaccagcaat cattacaccg ttcctatcca
     1801 ggatacgtcc tctcttacct ttcagctttg atacccttac agtattatct tcttcaagtt
     1861 ctggaaatat gacgtttgac tgccatttta ttttataatt tttctgtcta tctctagtta
     1921 atagtacttc atttgaaaat gaaatttcac ctgctaaagt atccatctta gaatcatagt
     1981 ttattacttt atcattattt ttattttttt taatatcatt aatcttaata cttatatcgt
     2041 gtgcatcaat tccttcatag atttttttat ttcttaaaac aaaatcttct tttttatttt
     2101 cagacttgct cttttcatct aataaatcat acattttctc atattgtttt ttatttgcaa
     2161 aagacatata ctgcttaaac acttcatctg gtttgttttt attgagtcca tctagtatga
     2221 attttgctaa aaaaacaata ataactataa gcagacacat tagaaaacca aaaattatca
     2281 tttttcttgt ttttttattt tttctcatat aaaccacctt tatcatttaa tatattacat
     2341 acgtaagctt taaggttaaa atactcttat agacataact aatctatgtt tttattatgt
     2401 ttttaagtat ctcataaaaa tatgtaagca gtataattga tagtaactta tatattacac
     2461 atgtaagttt taaaagtcaa taatattatc attatttaat tattttttac atcaacttaa
     2521 aaataaatca gttccattat attcttttgt attcttcaac tattttataa attaacctta
     2581 ttctattgta attattttat agtatataac tttaacttga caaaacgaca caagtgtctc
     2641 ataattaaaa gtgacactta tgtcgttaaa aaggaggtta aaacatatgc caacaaaagg
     2701 agaacaaact aaacaattta tcattgatac atcaataatg ctattttctg aaaaaggata
     2761 tagtaatgtg acaatgaaag atatatgtga agcatgtaat ttaagccgtg gtggcctata
     2821 tagacacttt aattcaccaa aagaaatttt tatcagtata ttaaacactc ataaatacta
     2881 ctctcaaaat ttgttggata aaggtatcaa ggaaaatata tcagctaagc atatgctaga
     2941 atattttttc caacaacaaa aggctgacat aaatggcaaa tattgtggat ttgattttgc
     3001 aatacacgaa tttgcttttg tagaaaaaga ccaacgtgat tatatgaata aaagatacag
     3061 ttcagctatc acaatgcttt caaatttaat cgattatgga caatctaaaa gtgaatttaa
     3121 aaaatgtgat tctaaatcag ttgcaataca cataatgttc ttatttgata gcatgaaaac
     3181 atctgcttcc attttgaaac ttacaaataa agacattgat aatcaaataa atattataaa
     3241 ggacatggtg attttatatg aataaacaaa aattccataa aattaattta caaaactggg
     3301 aacgatatac acatttttac tacttttcaa aaatgacaaa tgtagggttt aatttgacta
     3361 tagaaataga tattactaat ttgcttaagg aaacgaaaaa aagaaatatt aaattttatc
     3421 cagcgtattt atatacaata actacttgct taaatcgata tagagaacta aaaatcgcat
     3481 atgatgaaga tgaactcgga tattggaaca cgttaacgcc actttatgcg atttttcatg
     3541 atgataataa gactttctca tcaatatgga cagaatatga tgaaaatttc tcaatattct
     3601 atgaaaatta tttagctgat gttaaagaat atggaaaaaa tcatggtttt ctagcaaaac
     3661 caaatccgcc aagaaactgc tatactgttt cctgccttcc atggattagc tttaagagtt
     3721 ttactgtaca taatcatggt cttgaaaatt attttttccc tacaattgaa gcaggtaaat
     3781 actatcaaaa agatgaaaaa atacttcttc ctctttctat aacagtgcat cacgcaacta
     3841 ctgatggata ccatgtaagt tgttttattg attacataca aaaaatgtgc tttaatacta
     3901 agatatggct acactaaaaa tttatacaat ataaaaagaa ctgtcgcatg agcaaattaa
     3961 atttgctatg agaaagctct tttttactta caatagttaa tatgagatga attatttctt
     4021 atactttgaa cttaccaata gttaatatga aatgaattat ttctcatact ttaaaaagat
     4081 tgaattactc ttgataatct tcttttgtag tcattacata tcacctacta ctcaacacat
     4141 attctttgac ctatttttac atccttgttt atatccaaaa tatttacctt cttaccatct
     4201 ggaaacaata ccattgttaa agtagacttt cctaattcgt gtattctctt taaatcaaca
     4261 ttaacaagaa cgtctccctg tttaaccata tctccttgtt taacagcaac atcaaagcca
     4321 tctccattta atgcaactgt atcaattcca acatgaatta atacttctcc attagttcct
     4381 ttaatcccaa atgcatgtcc acttgaaaat actgtttcta atttacctga aataggagaa
     4441 acaatttttc catctataat atcaatagca aaaccatcac ctaacatttt tttagaaaat
     4501 gtttcatctg gtacattttc taatgcaaca attttcccct tagctggaga cacaaagttt
     4561 tcattatttt cattaaacac taattcttcg tcttcatctt catttatagc ttctttaggc
     4621 actccaaata aacatgttac aataaagcag aatgcaacca caaatattga tactaataag
     4681 tatgtgatta aattatatcc atcatagata tacattaaat aagaaggtaa aactgctaat
     4741 ccatatgaat tagattgaat atgtaaaatt gataatattg ctgctcctaa acctgaagat
     4801 gcacacataa taatcaaagg ctttaaatta tatcgaagct gaatcccaaa taaaacaggc
     4861 tcacttatac caaaaagttg agatagcatt gctccaactg cagtttgttt aacttgatta
     4921 tttttagatt ttttcataaa tgctagacat gttccaatat tcgcaaatcc atacattgca
     4981 caaagagtaa taagggcatt aaatcctgta tttgctaata gtgatgtttc aatcattaca
     5041 taagtatgat gtaagccagt aataacagct aatggatatg taaatccaat aataaatcct
     5101 ccaattccta aaggcaaatc aatcaataag ctgatgatat caaccatttt taattctatt
     5161 gtatgcatta tagggcctac acctaataaa ataactaaca ttgttattag cataacaaag
     5221 aatggtgtga aaataaggtc taatacatta ggcatatgtt tacggaacca tttttcaaga
     5281 ttagctccaa taaatgctgt aacaatagct gttaaaacac ttccttgaca acctacaata
     5341 ggaataaatc caaatgccat aattgcttca atttcatttg atgggtcagc aacagaatat
     5401 gcattaggaa gtattgggga aactaacata agtccaataa caagtccaat aacaggtgtt
     5461 cctccaaata ctttaaatgc tgaccataca ataattgctg gtaaaaaagc aaatgctgtt
     5521 tctgtaagaa ccgaaacaag tgtctgaata tacaatggaa tatttgcaga agacattcca
     5581 aataatccaa gtacattatc attaaacaaa caacccttta atcccaagaa taatcctgtt
     5641 gctgcaataa ctggaataat aggaacaaat atatctgcta gagttctcat aactttctta
     5701 aatccagttt cattattttt aactaattca tcttgttctt tttttgataa agtttttaat
     5761 cccatttttt caacttctgc ataaacttta ttaactattc ctgttcctaa aataatttga
     5821 tactgtccag aagtaaagaa aacaccttta acctcattga ccttttccac tttcttatca
     5881 ttaatagttt ccctatcttt aatcatcacc cttagtcttg ttgcacaatg tgtaatagaa
     5941 ataatattat cttcaccaat aattttaata agctcatttg ctattttatt atatttattc
     6001 atttttctac ctcgctttca ataatactgc atgaaatggt ttcaaacaca ctgttttatt
     6061 taatagattt atatcatcta aattttggaa tacaatatca tatttttctt ctactcttat
     6121 ttctacaggt ttatcagaaa aattataata acaatcaata actaccaaat ctaattgcct
     6181 tctatacgca ataacatctt cactttctat atcaataggt tctattgaac cataaatcaa
     6241 acaatcagaa tactcactat cttgtctcaa ctttatcatc tttttataaa attctaaaat
     6301 actctctgaa ttgttaatct gactctgtgc attaatatct ttataatttt caataacctt
     6361 taaccatggt gttgtatctg aaaaacctgc attttcttta tctgcccatg gaaatggcgt
     6421 tcttgcatta tctcggcttc ttaaattcac aaattctaac gcttcttctt ctgagtaacc
     6481 ttcatctatt gaacgatagt attggtcaat gctagataaa tcattaaaat catttatggt
     6541 atctctttta aaatttgtca ttcctaattc ctgcccctga taaataaatg gggttcctct
     6601 taaaaagaaa tacattgctc ccaacatttt tactgcatca tcattccttt cgtattgcaa
     6661 aagatacttt gttgtagctc ttggttggtc atgattttca ataaaattag ctgcccatcc
     6721 atgttcctga acatacattt gactatctaa aatgcatttt cttaaatctg gaattgtcca
     6781 tgaaattctt ttaaaccact cactaccaga agcaacatct aagtcagcat gtttaaagtc
     6841 aaagaccatt gaaaagaaac catcttctcc aataaaatca cctaactggt catagctaat
     6901 acctgctgct tcagcaacag tcatacaatc atatttttta aacgtatttt cttttaattc
     6961 ataaagcatt ttttctattc caggctgatt tctaactgtt tttgtacatt taacaagacc
     7021 atcaacacca tcactttcta aactttgaaa acttaaattc tttttgatga atgtaatagc
     7081 atcaactcta aatcctgcca ctcccatttg agaccaggta ttaatcattt ggtaaagttc
     7141 acttcttaat attggatttt cccaattaag gtcaggttgt tttttaccaa aagaatgaaa
     7201 ataatattcg tctctattct ctaccttttc ccaaacactt ccaccaaaaa cagacctcca
     7261 gttatttggt aaatcgtttc cttttttaaa aatataataa tcatgataag gactttctgg
     7321 attttttaat gcttcttgaa accaaggatg ttcatcactt gtatggttaa gaactaaatc
     7381 taatataatt ttgattcctc tatctttact ttctttaatt aactctttta aatcttccat
     7441 acttccaaat tcacttgcaa tatccaaata atcactaata tcatacccat tatcgtccat
     7501 aggtgattta aaaattggac ataaccacaa tgatgtaata cctaaatctt ttaaataatc
     7561 taaacgagaa ataattccct gtaaatctcc aattccatca ttattactat cttgaaagct
     7621 tcttggataa atttgataaa cgatttcttt ttgccaccat ttcttttcca taaattactc
     7681 ctttttatat gatatttctt ctttttaatt ctttataaat tccattatcg aatatgtcct
     7741 cacatataga tgtcgctata tctttaagtt gttgatgact atttttcatt gcaattgtaa
     7801 cgtctgatgc ttgaaacatg acaatgtcat tttgaccatc accaaaacat atggtttctt
     7861 tttgagttac tcctaatctt tcttgtaatc tcttaattgc tttgccctta tgaaaatctt
     7921 tttgaataat ttcataatac tgactagaaa tatctctctg tgctaattcc attttatctt
     7981 gtagtatatc tttcacttcg tcaaatacct tttcatttga ccataaacaa attttatgta
     8041 tatcttgtga cttatactct tcaatgttat tttcatatgt tattttttct tggatatgtt
     8101 gtttatttat acaagaatta gttcctttca actgattcat tgtttcaaat atttctttgg
     8161 ctttttgatt catgaaaact ttttcctgac tctcaatcga aaaagcaact tccctctttt
     8221 ttaataaaca aacaacttcc tttatcaatc tttgattaaa agattgatta taaagtaatt
     8281 caccatgata ttgaatgtaa ttacctcctc ctgcaatata tccatcaact cctaatgata
     8341 aaacatcatc ttgtattgta cccatacttc ttccagtaca aataacaaca ctacaatgat
     8401 tcttttgaca taaacgtata gcatgctttg cagattctgg gattccatat acctcatctc
     8461 taagcgtacc atctatatca agtattaata atttgtacaa tttcatcttc ctttcttact
     8521 atcaaaatca taatagcatt caaaaaaaaa gatatcaata ttttgatatc ttttaaaaaa
     8581 tatgttcatt tttagcacaa attttcattt tcggggaata tgttcacttt ctaaatacat
     8641 cttgtattta atataaagca gttcaattaa aataaaataa ggtgttgtta catcgtagtc
     8701 cagttgttta tctacagaaa tagatataga ttgaatatac aggttttcat cacataatct
     8761 tgctaaagga ttacttatca tttttgtaat agaaaccata taaattcctt tcattctcat
     8821 ttctttagca aaatcaataa ttgtagatgc atctccattc aatgaaataa agaaaatcat
     8881 atcatctgtt tttataaaat gattaagtgc ctgaatcata tcataaccat aaacatcata
     8941 tataattttc cctgttggta agaaaatcct tttcatctct ttagcaactc tttcctgaga
     9001 ataaccttct ccaaagacga ttatacgatt tgcctttttc attctttcaa acataatcga
     9061 acaatccttc ttctctatat catctatcac cttattataa caatccatta attggtgttt
     9121 actttgattt ttatattcta cttctgtttc atttaagcgt ataataactc ttaattcact
     9181 atatccaggt aattgtagtt tttgagcaaa acgagaaagt gcacttgtag atatatggta
     9241 tttgtctgca aattcatcta tagaaagttt aatacaatct tctttatgat taagaataca
     9301 ctcacagata tacttatcat ttgctgtaaa tgaattataa tatttattga aaagtccttc
     9361 aattttcatt cttttctcct tctatactct atgtaattca tatgaagcaa atctaataga
     9421 gcataaaatg ctcctacgac ttcataagat atagcttgta atgtatcaag tttttgagtt
     9481 tcaacataca gattatactc acaaagtgtt gcaattgtat tattatcaag cctagttaaa
     9541 gaaatcgtat gtatatgatt gttgataaat tttgcaattt caattcctgc ttttgtttct
     9601 ccagataaag aaataataat aaataaatca cttttttgaa atgattctct cataaattca
     9661 atttcaccta tatcaaataa gtcaataaca catttaccta ctgaaagaaa tattctcttt
     9721 atttcttgag ctaaactttt ctgttcatta cctgtcccat aaatataaat tgtttctgca
     9781 tcatatatta ttttacaaat atcttgaaat gatatttttt gtaattcatt cattaatagt
     9841 cgataattct cgcaaacatc atgaaaatta ttatcattat taacaaattc tagttgatta
     9901 tttttaagta aatactttaa atcagagaat gaatctaatc caatttttct acaaagcctt
     9961 aataaagtag ctctagaaac attacattca tttgcaattt tttcacacga aaaattacgg
    10021 tattctcttt gacaaatcaa tatttgttta ataataattt tatcattttc atttaaatta
    10081 tcataatttt ggttaactaa cgattctaat ctcatttttt ccactcccta aatattattt
    10141 taatatacat tagttgcttt ttctagggaa gagttttaaa taataaggat aaaataatca
    10201 tttgatttta tttttattaa aataaaaata acctgctaaa agcaggcacc aaaaagtact
    10261 tatcctttcg aacatgtact ttagatattg ctttcttcgg aagcttcatc tcttcatact
    10321 ctaaaataga gttttttcta actcaaaagt attttccatg gtagaaatta ctttaattca
    10381 tctgccattt attttttatc ttatttaata attactacta cttgaatatt tacttcttta
    10441 agtaaatttc atagttaaca aagctaaaac tattattata gatgttaaaa tacaaaattg
    10501 taagtattgt tataaatatg cttacagcta caaatataca tgatatatta aaaagaagct
    10561 gtaaattatt atcatacata caagcaacta cgcctctatt aatccatgtt aatttttatt
    10621 atttaataca aggagttcca tgagacatac atatccattc atattttaaa ttatccaatt
    10681 ctcttataga tttcatcatc tttttataat cccaattcca tggattagga taaggaacta
    10741 tatttccatt cttatttttg actaaatcac ctgcaaataa tacatcttca aaaatcatgc
    10801 agacatgccc tggagtatga ccaggagtat ggataatttt tatattccca atctgcatat
    10861 tctctccata aggttcaaca tccttaatga gccttctgga aattgccttt ccaataaact
    10921 tcttaaaccc aggtctatcc ttctctccta taataaaagg tatatcctct acatgagccc
    10981 atacttttgc tcctgttttt tctttcaata atttaatatt agatatatgg tcaacatcat
    11041 gatgagttaa taaaatatgc ttaatatctg taagtttaat accaagtgtt tttaattctt
    11101 taattatatt tttccccata aaaggaagac ctgtgtctat aagaataatt tctttatcaa
    11161 acacaataaa acaatttgag ccacgagtac tatctaacat taatacattt tcttttatct
    11221 tcataaataa attcccctta tttggtattt tacctatata att
//
LOCUS       DAENCV010000118        11204 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAENCV010000118 DAENCV010000000
VERSION     DAENCV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.119"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            120..1601
                     /locus_tag="KQD82_003044"
     CDS             120..1601
                     /locus_tag="KQD82_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG4381500.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1634..5109
                     /locus_tag="KQD82_003045"
                     /pseudo
     CDS             1634..5109
                     /locus_tag="KQD82_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            5127..5873
                     /locus_tag="KQD82_003046"
     CDS             5127..5873
                     /locus_tag="KQD82_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG4381501.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5822..6142)
                     /locus_tag="KQD82_003047"
     CDS             complement(5822..6142)
                     /locus_tag="KQD82_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG4381502.1"
                     /translation="MKKIMNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            6930..11072
                     /locus_tag="KQD82_003048"
     CDS             6930..11072
                     /locus_tag="KQD82_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG4381503.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPDGNYRIVEAFGTTGGVPTPGDFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNASTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTTVPGETFSYTITVTNLGPSAAQSIVLIDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWTGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEVTSTAPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCYCNCCKNDF
                     WC"
BASE COUNT         4521 a         1343 c         1780 g         3560 t
ORIGIN      
        1 tttatttaaa tactctttat taacaattta taaaacttaa ttgtaaaaaa tacttattaa
       61 tagtaaaata aagtgaatca attatattat aattgctaaa gagagtgagg tgtagctatt
      121 tgaaaaaaaa tagattaata gcaaagaata tatcaatagc atttatagta ctattctttt
      181 ttagtgtatt tacatttttt tatgtaggta atattaatag agtgttagaa tatgaaacaa
      241 atgacataat aacagttaca attgctggat ggattatact ttcattttta tttttaggta
      301 ttataattta catattatat tcaaaagcaa atagtcaaaa aactatagaa aaagtagcat
      361 atacggattt tgtaacaggt tattccaact ggagaaagtt tgaattagat gttacaaatt
      421 tattaaagaa aacatcacaa aataataaat atgcaatggt catattcgat attgataaat
      481 ttaaagcaat taatgatata tatggccata aaaagggaaa tttaatatta aaagatattg
      541 cagatacttt aaatgaattg actgatatca atgaaacttt tgctcgtgta agtgctgata
      601 atttcaatat attattaaca tataacaaaa aagaggatat aataaatatt atcaaaaaga
      661 ttatggcaaa taatgaactt gtaaacttat cttttggaat ttatgaaatc aaagataagg
      721 atttatctgt aagtgtatat agtgatagag cttcacttgc aaaatcatca ataaaaaata
      781 atagtgatgt aaattttgca ttttttaatg acaaattaag agagaaatta ttatttgaag
      841 ataagataga aaaagaaatg gaatatgctc ttgaaagtgg gcaattcgta atgtatttac
      901 aaccaaaata caatattaaa ttagataaat tttgtggaag tgaagcccta gtcagatggc
      961 aatatacaga aaaagaggtc atatatccag gtgattttat tcctatattt gaaaaaaatg
     1021 gatttattag aaagattgat atgtacatat tagaacaagc ttgtaaagaa atccggtctt
     1081 tatttgataa aggaatttca cctttaccta tatctgtaaa cttctcaaga gttgattttt
     1141 ttaaaaaaga ttttattgaa aacatagtaa atatatgtga tagatataaa ataccatata
     1201 gtttaataga aattgaaatt actgaatcta gtatgtttgg agatactgat actctattta
     1261 atgttagtag aaacctacag gatattggct ttatagttgc aatggatgat tttggctctg
     1321 gatattcttc tgtaaatatg cttaaaaata taccattaaa tgtaataaaa ttagatagag
     1381 gattttttgt tgatgataaa gatgttgata agagtcaaat tgttataaaa agcatagttt
     1441 ctctcattaa gcaattagga ataagggttg ttgctgaagg aattgaaaca agaagtcaaa
     1501 tagaaatgtt aaaaaaagca aattgtgata ttgttcaagg atattatttt tcaaaaccat
     1561 taccaattaa agaatttgaa aaattagtct ataaaatata gtcagcgagt cttaaatggg
     1621 aggtggtgat taagtgataa gagcaaaatc gttaaaaaat gtactgagta ttgttttaat
     1681 atttttattt acaataattt ctttttctga ttttgcattt aaaactaatg tccaggaaga
     1741 agaaaatagt aaaattaatt taagagaaat atccctacaa agttccaatg ctataaaaat
     1801 aaaattagat agttctctat cctttattaa aagtgtggca aatatggttt cttattttaa
     1861 tactatagat aactcaaaaa tagttccatt attagctaat atatcaaaag aatctgactt
     1921 tcaaagaatc tctattgtat ctcctgatgg aataagtcat accagtgatg gcaaaattat
     1981 caatgtgtca aagtatcagt attttaagaa agctattaaa ggagaaagta atatatctga
     2041 agttgtgaaa tctccaaagg agaaagtaat atatctgaag ttgtgaaatc tccaattgat
     2101 ggaaaagata cttttgttat agctactcct gtatttaata agaataacaa aataattggt
     2161 gttttatacg gttcttacta caatgataaa ttagctgaat ttatagatat aacaagtttt
     2221 aatagtgaag ggtacataga tatatttgaa tctagtggtc aatttgtttt aaaatcttca
     2281 catacaaaca aactgattaa gtcaagtagt aacatatatg atatatttaa aaaagcttct
     2341 tttgatgatg gttatagcta taaaaaaata tatgaagata caaaaaataa caagagtggc
     2401 tttacctcat atgaatacaa aggagaaaat cgttatgcta cttatacacc cattggtgta
     2461 aatgactggt atgtaatttc tgtgatacca caatatactg taagaagaaa cattgataaa
     2521 ataaatcaat tagcattttc tcttattttc aaggtattct tattgtttat tatgcttata
     2581 atatatatta tttataaaaa caataaatct cagaaagata tactaaaagc taacaaagaa
     2641 atctcatcta ttacaaatag cattcctgga ggagtacaaa agtgtaccct gggtgagaca
     2701 tgcaatatta attttttaag tgatggattt atagagttgt ttggttatac aagagaagaa
     2761 ataaaaacat tgtttaataa tgatttttat agtttaatac ataaagatga tgttgaaaga
     2821 ataaaaaaag aaatttttaa acaactttac aaaaataaaa ctgttgaatt agaatatagg
     2881 attataacaa aaaatggtaa tacagtatgg gttttagata aaggggagct tataacagaa
     2941 cctgatggaa aagagtattt ttgttgtgta actgtagata ttactagaac aaaacaatta
     3001 gaacaagaga tggaacaaaa aaatctagaa cttgaaatga tatcagaaaa tgttttaggt
     3061 ggtataatta taacagaatt taatgatgat tttactataa aatacgctaa ctctggttat
     3121 ctttcaatga ttggatatac acgtgaacaa ttagaaaatg aacttaaata caaagcttgt
     3181 aatcttgttt tttatgaaga tagaaagaag gctcttgaaa gtatacaaca tcaattaaac
     3241 acaaaaggaa tagctgtata tcaacataga ataagaagaa gagatggtaa tattatttgg
     3301 gtttctataa aaggaaggct tattgttgat gatgatggta ttgaaaaagg tatttggatt
     3361 ctaaatgata taacagattt aaaaaatgca gaacaaaaac ttaaaataaa tgaagaaaga
     3421 tttagaattg cattaaagca aacatcaaac actatatttg agtatgatat aaaaacaaag
     3481 tcacttttaa atactagtaa agagcttgat aaatataata tttataaaat tactaaaaat
     3541 gttccatatt ctctagtaga atcaggagtt attcataaag attttagtga tgattttata
     3601 aacatgtatg aaaatgtaat aaatggagca aaaaaagcaa gttgtgtagt taagatgaaa
     3661 cttccagatg aaaattatat atggaataaa gttaccttga ctactatttt tgatgaagat
     3721 ggtaatcctg taagagcaat tggtatacag gaagatataa gcaaacaaaa ggaagcagaa
     3781 ataagataca atcaagaagg taagtatcat tcagctatat tgtcggaagc aatgataacc
     3841 tatgaaataa atgtatcaaa ggatactttt atcaaaggaa atgaaagttg gcaaaaacca
     3901 tttggaatta agcctgacaa taactattct caaatggtaa aaactatagc aaatagtatt
     3961 gtacataaag acaataaaga aacattttta aatacatttt tatgtgaaaa cttattggat
     4021 gcatataaaa ataatgtgac tgaaataaaa ttagaatatc aaagaatgaa tcaagaaaat
     4081 aaaatgattt gggtcatttg tacaatgcac ttaataaaag attctattac gaacgatata
     4141 aaagcacttg cttatgttaa agatattgac aagcaaaaaa atgaagaaat tagattgaaa
     4201 ttaaaagctg aaagagatac tcttacagga ttatataata aaggtactac agaaaaattg
     4261 attaatgatt tcttgaactc tgaggaagca agcttaggaa tgcatgcatt tttaattgtt
     4321 gatatagata attttaaatc tgtgaacgat aatttaggac atgtatttgg agataaagta
     4381 ttatttgaag tttctaaaaa cctaaaacct atatttagaa aagatgatat agtaggtcga
     4441 attggtggag atgaatttat agtattctta aaaaatattc agtcttttga gaataccaaa
     4501 aagaaagcag aaaaaatatg cgatatattt agaaattctt atacggaaaa taaaaagaca
     4561 tataaaatat ctggaacagt tggaatttca tttgtaccag aacatggaac tgtatttgaa
     4621 gaactttata aaaaagcaga catagcactt tattatgcaa agagcaaagg aaaagataag
     4681 tatgcacttt ataatgattg tatctaccaa aatggacttg aaaataatat aagtgataat
     4741 aatataaaat tagcagataa atgtgagaaa tccaaaccag tacttaaaga aattcttgat
     4801 tctattgata attatatcta tatagtgaat ccaaatactt atgaacttct ttttgtcaat
     4861 aataaggtgt ctcgtttgga tttagagata aaattaggag aaacctgtta taaatcacta
     4921 ttaaaatctg atagaccatg taataattgc cctcttaacg gattagaaga aaataagcat
     4981 tcaaaattca aaatagagac aaatacattt ggtaaaaata tcagtatagc aagttggatt
     5041 aattgcatag atggagaata caactgtctt atagagtgta tagattcttc tttatacaaa
     5101 aataaataaa aaaggactat gaagatatgt taaatagtat aactttgata cttggtataa
     5161 tatttacaat ttactatttt tatctcaaaa tagtttttgg aggaatttca tttagtgaga
     5221 ttttcttagt tgtaggaatt gtactaatta tctatcaaat atttaagaaa aaaattaaag
     5281 aaaagaaggt tttttatagg gtgttaaaaa taataatcag cattttttta atagtatttg
     5341 tagttactga gagtctaatt attttttatc ctaaaaatag tttagaatca aaaagtgatt
     5401 atctcttaat tttaggagct tctgtaaaaa agactacacc aagtacaacc ctgaagggta
     5461 ggttagacac tgcattgaaa tacctaaaaa ttaatgataa ttgttatgtg gtagtatctg
     5521 gtggtaaagg aagtggagaa aaaataactg aggcaaaagc tatgaaagat tatcttataa
     5581 aaaatggtat agataaaaat aggataatag aagaggataa atccacgaat acttatgaga
     5641 attttaaata ttcaaaattg aagatagaag aacatagtca aagaaaacta agcgatttaa
     5701 aagtaaaaat agtaacaaca gattttcatg ttctcagaag taaaatatta gcatatagaa
     5761 atggatataa aaatacgtct ttttatgcta gtaaatcaaa actttcattt gtaccaactt
     5821 attatacaag agaatttttt gcattatgga aaacaatcat ttttgataga taataaataa
     5881 aaagactaga tataaactca tatctagtct tttctattcc cttaattcta atccattttc
     5941 caactttact tgttgtagaa atttcaacat tgggatttac aagtaattgc ttataagaag
     6001 ttttatattt tacgattcca aaatataatt ttccttcata atccattata aatccgaatg
     6061 gtcttactct tggtttatta tcttcaactg tagcaaaata gaatgttgag ttttctttaa
     6121 aaaaattcat tatttttttc atgattaaat cctctttgta tcttatacta atataatgaa
     6181 tataaataaa atattcataa tactaatata aatcgatata aaaacaatta caatattata
     6241 ttgattattc aaaattatat ataaaattca gaaaaaaatt agtttacata ataaaaataa
     6301 agtcaactaa aattattgat tttagattaa agtaccagat taagcgttta agtagatttt
     6361 aagcttgatt tgattagaaa agtacaaact atcgccgaaa aaaattaaac ccatttaaaa
     6421 agcaatataa aagcttgtta tttttgagaa aaatagaccc tctaaactca attagataaa
     6481 aataaaatac agaaaattat atctctgaat atctcaagta attaatttta aattttacga
     6541 tattttgttt gatgatttat aatataaaag catactctta atctgctcaa attaaaaaga
     6601 ataaaaaata acaaaaaaat cactgacatt tcactgacaa attgtaattt tgttattata
     6661 aatacattga aaatacttga ttattgaaaa atggcataaa ataatacatt ttatgccatt
     6721 tactattatt tgatagtttt taacttatga cataagatat tttttaacat atacaataaa
     6781 atataaaaaa ttgatattac aaataaaaaa aatacatatt agatgttata gaaaaaaagc
     6841 gcacttatag gcttaaaatg tcacataaaa gagttttaaa gaataatata ttatgaacac
     6901 aaattttgta atcgcaaagg agtgataata tggcaacagt atcaggacaa atagtttttg
     6961 atagaaatag aagtgctacc attgattctg gagactcagg aatagccaat ataccagttg
     7021 tattacaaga tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt
     7081 atgtttttac taatgttcca gatggtaact atagaattgt agaagctttt ggaactacag
     7141 gaggagtacc aacaccaggg gattttgcaa cagcgatagt aggtccagtt ccaattgcag
     7201 aaatgccacc tattagttct gtgacaaatc ctcctattgg ttcaacaaac ttagatggta
     7261 caacacctaa cacactatta ataacagtag caggttcaga tataacaaat caaaatatat
     7321 taaatgggcc tgtgatttat acacctatag agaatatatt ggattcttgc gtatctgtat
     7381 ctaatataaa ccttattaca gatgcagata acggaacatt cggttttttt ccaccaggta
     7441 caccaagtaa tacaggacct gaaatagaac cttacccaaa tgtgacgcca gattttacgt
     7501 atgttgtacc agacccaact aagtttacac ctttagatgg agaatataca gtacaaaata
     7561 ttatgacaaa tgcaatgagt aatgttatag gagcttggtg gcgtattgct gaccatacta
     7621 caggaaatga aacaggaaga atgatggtgg taaatggttt taatcctgga gctgtatttt
     7681 ttaaatcaac agtacaagtt acacctaaca ctaattattt atttagcact tggattttaa
     7741 acctttttaa ggtaacagga tatccaccac cacagctagg tgtaagaata tttgaccaaa
     7801 ataataatat actatatcaa gcaacattag gagctcaaat tccagttaat acaaacgcac
     7861 cagaatggaa agaagtaggt actgtgatta attctcaaaa taataatcaa cttacagtag
     7921 aattttttag tgaaggagaa agcgccattg gaaatgacta tgctatagat gatgtttctt
     7981 taagagaaat agctgttcca acttttactc caataaaaac aagtaacaca gaaactgcta
     8041 atgtaggtga tataatcaca tatacagtta cattagataa tacttgtgat agccctttaa
     8101 ctaatgtatt ttttagggat ttaattccaa atggtttaga gtttatacct ggaactgtaa
     8161 cggttgatgg agtatctgta ccaggagtag accctaatac aggtttccca cttccagata
     8221 ttggaggagg aacaggggtt gaagtaactt ttgatgttgt ggttgaaagt attcctaacc
     8281 caaatcctac taataatata gctaatatag attactccta tacacctgta gaaggcggta
     8341 taccaaatga tttttcagta gattctaatc cagttcctgt tgaagtaata tcagcagata
     8401 ttgaagttac aaaattatct gaacctacta tagtgaatcc aggagaggaa ttaatataca
     8461 ctataaaagt agtaaataat ggaccattcc catctgaaaa tgtggtttta acagatgatg
     8521 taccagcttc tattgtaaat ccagagtatt cattagatgg aggagtaact tttcaaccat
     8581 ggactggaag tttaaatata gggacattag aagtaggaga gacaagagtt attataatta
     8641 ggggtattgt aaatccatca acagtaggaa ttataacaaa tacagctgta gtatcttcaa
     8701 ctacagcaga cccaaacctt aataataatg catctacaat agagacagaa gtgaatttat
     8761 tagcagatat tttagtaatg aaaacagcag aacctaattc agctgttcca ggtactttat
     8821 tgagatatac tattcaagta gaaaacttag gaccagcaaa tgctgaaaat gtaatattaa
     8881 atgatgatat tccagcttct attataaatc cagaatattc attggatgga ggagcaagtt
     8941 ttcaaccatg gaatggaagt cttaatatag gtactttaaa ctctggaatt tcattaacag
     9001 ttttaattca aggaacagtt agtttaaatt cttcagaata tatagtaaat acagcaacag
     9061 tttcttcaac tacacctgac ccagatttaa gcaacaatat atctaccatt attacaccag
     9121 ttaacccaca agcaggtatt tctattataa aagtagctga cgaggatgta gctgttccag
     9181 gagaagaatt tgtatataca attgaaattt ttaatgaagg accatctaat gctacgaatg
     9241 tagttttaac agatgatata ccagatgtta ttttaaatcc agaatattca ttggatggag
     9301 gagcaacttt ccaaccatgg actggaagtc ttaatatagg tacagttgca ccagggcaat
     9361 taataagaat tatcataaag ggattagtaa gttcaactgc tacaggagat attactaata
     9421 cagcagaagt aagtgcagat gtaccagaac cagttacgga ttcatcaaca gtgacaactc
     9481 ctatagttcc atcagcagat atagaagtaa taaaaactag caatatggat acaacagttc
     9541 caggagaaac tttcagttat actataacag ttacaaattt aggaccatct gctgcacaaa
     9601 gtatcgtttt gatagatgat ataccagatg ttattttaaa tccagagtat tcattggatg
     9661 gaggagtaac tttccaacca tggaatggaa atcttagtat aggcactttg gatgctggag
     9721 aaataagaag tattataata agaggaacag tgagccagac agcagtaggt acaattataa
     9781 atacagcaac aataagttca actacacctg acccaaatcc agataataat acttcaacag
     9841 atgaaacaga tataagtcca ttagcagata tttctgtgat aaaaggcaac gaaccagttg
     9901 ctataccagg gggaagattt atttatggaa ttgaaattgc aaatgcagga ccatcttttg
     9961 cagagaacgt aactttaaca gataatatac cagcttctat tttaaatcca gagtactcaa
    10021 tagataatgg agtgactttc caaccatgga atggaagcct taatataggt actttagatg
    10081 ctggagagat aagaaatatc ataataaggg gaacagtaag tcaaacagca ataggcacaa
    10141 ttataaatac agcaacagta agttcaacta cacctgaccc aaatctaaat aacaatactt
    10201 caacatcaga agcagaggta agttcatcag cagatatttc tgtagtaaaa cgaagcaatc
    10261 agactgttgt tgtaccagga gatgtactag actatacaat tgaggtgaga aatgcaggac
    10321 cgtctactgc tcagaacgta actttaacag ataatatacc agcttctatt ttaagtccag
    10381 aatattcaat agataatgga gtgacttttc aaccatggac tggaagtctt agtataggca
    10441 ctttagatgc tggagagata agaaatatta taataagggg aatagtaagt caaacagcaa
    10501 taggtacaat tataaataca gcaacagtaa gttcaactac acctgaccca aatccagata
    10561 ataatacttc aacatcagaa atagatataa gttcatcagc agatatttct gtaataaaat
    10621 tagcaaataa aacagaagca tgtgtaggag agcaaataga ttttgtaata gtagtatcaa
    10681 atgcaggacc agaaaatgct caaaatgtta ctttaataga caatgtttca gataagctta
    10741 agaaagcagt attttcactt gatagaggtg taagttttca accatggact ggaagtctta
    10801 atataggtac actaccagct ggaactttaa gagtgattct attaagagga attataaaat
    10861 caacatgtct tgatagatta acaaatattg cagaagttac ttcaacagca ccagattcaa
    10921 accttaacaa taatatatca agagtgcaag tagaaataaa acaatgttgt tgtaatgatt
    10981 gctgttgtaa caactgtagt aattgctgtt gcaatgattg tggttgtaaa gattgttact
    11041 gtaattgttg taaaaatgac ttttggtgtt gattttataa aataattttg tataccttaa
    11101 attaaactga aataaaaaat aaattcacat atgagtaaaa tattttataa atattaataa
    11161 attttctatc tttgatatgg accacattcg taatactagt tatt
//
LOCUS       DAENCV010000119        11107 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAENCV010000119 DAENCV010000000
VERSION     DAENCV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.121"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..371
                     /locus_tag="KQD82_003049"
     CDS             <1..371
                     /locus_tag="KQD82_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG4381504.1"
                     /translation="PHCIQENSCKLKVTNDIDNCKECGRCNIGELIKLKEKTNVKIFV
                     ATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNKRPNGPCVNTNVD
                     MMDIEKAIGFLTGKNILVCN"
     gene            440..1141
                     /locus_tag="KQD82_003050"
     CDS             440..1141
                     /locus_tag="KQD82_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG4381505.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            1203..2528
                     /gene="rsmB"
                     /locus_tag="KQD82_003051"
     CDS             1203..2528
                     /gene="rsmB"
                     /locus_tag="KQD82_003051"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG4381506.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFILDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQGVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            2536..3567
                     /gene="rlmN"
                     /locus_tag="KQD82_003052"
     CDS             2536..3567
                     /gene="rlmN"
                     /locus_tag="KQD82_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG4381507.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            3587..4339
                     /locus_tag="KQD82_003053"
     CDS             3587..4339
                     /locus_tag="KQD82_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG4381508.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            4356..6371
                     /gene="pknB"
                     /locus_tag="KQD82_003054"
     CDS             4356..6371
                     /gene="pknB"
                     /locus_tag="KQD82_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG4381509.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            6479..7216
                     /locus_tag="KQD82_003055"
     CDS             6479..7216
                     /locus_tag="KQD82_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG4381510.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            7314..8198
                     /gene="rsgA"
                     /locus_tag="KQD82_003056"
     CDS             7314..8198
                     /gene="rsgA"
                     /locus_tag="KQD82_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG4381511.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            8203..8874
                     /locus_tag="KQD82_003057"
     CDS             8203..8874
                     /locus_tag="KQD82_003057"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG4381512.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANIIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            8883..9524
                     /locus_tag="KQD82_003058"
     CDS             8883..9524
                     /locus_tag="KQD82_003058"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG4381513.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            9655..10284
                     /locus_tag="KQD82_003059"
     CDS             9655..10284
                     /locus_tag="KQD82_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG4381514.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            10339..10995
                     /locus_tag="KQD82_003060"
     CDS             10339..10995
                     /locus_tag="KQD82_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4381515.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILAEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         4407 a         1136 c         2174 g         3390 t
ORIGIN      
        1 tacctcattg tattcaagag aatagttgta aattaaaagt tactaatgat atagataatt
       61 gtaaagaatg tggaagatgc aacataggag aattaattaa gctaaaggaa aaaactaatg
      121 taaaaatatt tgtagctaca ggaggaaccc tagctagaaa aattataatg gacactaaac
      181 ctaaagctgt tgtagctgtt gcatgtgaaa gagatttgac atctggaata caagatatta
      241 aaaaaattcc tgttttaggt gtatttaaca aaagacctaa tggaccttgt gtaaatacta
      301 atgtagacat gatggatatt gaaaaagcta taggcttttt aacaggaaaa aatatattag
      361 tttgtaatta aactgttttt aagttaagtg aatattaaga ggatgtataa taaaaatata
      421 ttttaggaga gtgttattta tgtaccctgt atatggcggt ttttggggat ttgacccaac
      481 aatggttgta ttaattccag ctatattact tactatatat gcacaattta aagtgagttc
      541 aacaacaaat aagtatttaa gagtaaatac acgtcgagga tatactggag agcaaacagc
      601 aagaagagta cttgattcaa atggtttgta tgatgtaaag atagaaatgg ttagagggca
      661 tctaagtgac cattatgacc caagaagaaa ggctgtaagg ttgtcagaag atgtatatta
      721 tggaacatct ataacttctg ttgcagtagc tgcacatgag tgtggtcatg ccatacaaca
      781 tgctaaaggt tatgcacctc ttcaaataag aagtagttta gtaccagtag tgaattttgc
      841 ttcaagtata tcttggtttt taatattttt aggatttatt atggctggtc catttttaaa
      901 aattggtata ctattgtttt cggcttcagt gttattccaa ataataacat tgcctgtaga
      961 atttaatgct tcaagtagag caatcgttca acttgggaat ttaggtatta tagatgaaag
     1021 tgagtcaaga caaagtagaa gagtactttc agcagcagct ttaacatatg ttgcagcagc
     1081 attggtttca atacttcaat tacttagatt gttactaata gctcaaagaa gaaatgacta
     1141 aaagtaaagt gggttgttta gaaacaaccc ttttttatgt ttaataaaag ggagaaattt
     1201 atatggatgc aagagaagtt ggatttaaag tattatgtga tatagaaaaa aatgacaatt
     1261 attcaaatat tgcaataaat aagcatttta aaaatttaga aataagtgat atggatagag
     1321 gacttgcaac agagttaatt tatggagtaa tagaaaataa atattattta gattatataa
     1381 tcaataaact ttcaaaaata aaggtaaaaa aaatgtcaac ttatgttaaa atctttttaa
     1441 gaatgggaac atatcagatt ttatttttag atagtatatc agattatgca gcagttaatg
     1501 aaactgtaaa attatctaag aaatatgata aaaaatcttc tggtttcata aatgctattc
     1561 ttagaaatga aataagagct aaagagacta taatggacat aactgaggaa gatagtgtaa
     1621 aatacttatc tataaagtat tcctataact cttggattat aaaaaactgg atagataaat
     1681 ttggacaaga atttgctgaa gatttgttgg aagcaaataa tgaaaagcct agtatctata
     1741 taagaacgaa tacacttaaa ataagtagag aagagcttat tgaaaaatta agtgaaatgg
     1801 gtattgtatg tttaaaagtt cctatggtag aagaagctat taaagtcgaa aaaatgaaaa
     1861 atatagaaaa aaatgaatta tttaaggctg gtttatttac aattcaagat ataagttcta
     1921 tgatagtagg gaaagtaatt aatccaaaag aaggttcatt tattttggat gtatgtagtg
     1981 ctcctggagg gaaatctact cacttagcta ctttaatgaa caatactggt caagtagtag
     2041 ctagagatat ttttgagcat aaactaaagc ttataaactc tactgtaaat agattggggc
     2101 ttaaaaatgt gtttgtagaa agatttgatg cttctgagat tgatgaaaat agtataagta
     2161 aatttgatta tgttttagca gatgttcctt gctctggttt tggtataata agacgtaaac
     2221 cagaaattaa gtataagaaa gaagaagagt taaaggacat cacttctata caaaagaaaa
     2281 ttttagaaaa tgcatctaaa tatgtcaaag ttggtggaac tttagtatat agtacatgta
     2341 caattcaagg tgtggagaat ataaatatag ttacttcttt tgttgaagaa aatcacaact
     2401 ttgaatttgt tccaattgat acagtaaatg tagacttaga caatcaagat aaaggatatt
     2461 taaagatata tcctaatatt catggtatag atggtttttt catagcaaaa ttaaagagaa
     2521 taaggtagtg agagaatgga aaagaaaaag atagtattaa aaaattttac tgaagatgaa
     2581 cttaaagaat ttatgaaaac tatagatgaa aagcccttta gaggaagtca aatattctct
     2641 tggatatata agggtgcaaa aacttttgat gatatgaaca atataccaaa aagtttgaga
     2701 aataaattag aagaggtttc atgtataggg catatagata tagaattaaa gttagaatct
     2761 aaggtagata atacaaaaaa atatttgttt ttattagatg atggaaatat aatagaaact
     2821 gtgatgatgg attatgatag tagagttact gtctgtgttt ctaaccaagt aggttgtagg
     2881 atgggatgta atttttgtgc atctacaatg gatggattaa ttagaaactt agagccatgg
     2941 gaaattttag accaagttat taaaattcaa gaagatactg gtaagagggt atcaaatctt
     3001 gtattgatgg ggagtggtga gccacttgat aactttgaaa atacaaaaca atttttaaaa
     3061 ataattaacg aaaaaaatgg tcttaatata gggtatagac atataactct ttcaacttgt
     3121 ggaatagtac ctaaaatgta tgagttggca gacttagaaa tagctataaa tttagcttta
     3181 tcacttcatt caccatatga tgaagaacga agaaaaatta tgcctgtagc aaatgcttat
     3241 tctattgaag aaatattgaa tgcatgtaga tattatataa aaaagacaaa tagaagggtt
     3301 acatttgaat attctcttat aaaaggggta aatgactcag aaaaagaagc aaaggcatta
     3361 gctaagctcc taaaaggtat gctatgtcat gtaaatttaa ttccaataaa taaagttgaa
     3421 gaaagagaat atgaaaagcc agataaagca tttatttata aatttaggga tagtttggag
     3481 aagaacaata tacctgccac agttaggatg tctatgggtt ctgatattag tggagcttgt
     3541 gggcaattga gaagaaaata caagtaattt gtaaggagga agtcgtatgg tttatagttg
     3601 tgcctcccat ataggaaaaa taagaaaaaa caatgaagac tattgtgagg gagaagttat
     3661 agatacagaa catggtccaa taggaatatt tgccatagct gatggaatgg gtggacataa
     3721 aaaaggtgaa gtcgccagta aattagcagt agaaaatata attgattttc taaaggaaaa
     3781 cttattacaa catgataatg taaaaataga ctatatagat gatatattaa aacaggctta
     3841 taataatgta aactcaattg tgcacaaaaa atctatggaa gatatagaat ttgaaggaat
     3901 gggaactaca ttagttacgg ctatagtgta taataatgtc ttatatgtgg caaatgttgg
     3961 agatagcaga tgttatttgt taacagatga aaaatttgat aaaataacta tagaccattc
     4021 cgtggttgaa gaacttatga tggcccatgt cattacagaa gaagaggcaa gaagacatcc
     4081 acagaggaat agaataacta gagcaattgg aacagatgat atggttgttg tagatatttt
     4141 taagaaagaa atcaaaaaaa gtgatataat acttcttgcg acagatggat taactgggtt
     4201 tattgatgat gaagatataa gagatttaat attagattat gaatatgaga gaactagtaa
     4261 tataagtgaa gaattgataa gtatggccaa tgatgtttct ggtaaagata atgtttcggt
     4321 tatagtgata aaagtataat taggacggtg atattgtggg agatacaatt ttaggaaatc
     4381 gatatgaaat catcaggaag attggtgatg gaggaatggc ctttgtatat gaggctaaag
     4441 acagattatt aaacaggact gttgcactta aagtacttag acctgaattt gtagatgatg
     4501 atgaatttct aacaaagttt aaaagagagg cagaagcagt agcaagtctt tcacatccaa
     4561 atatagtaaa tgtttatgat gttggtgaag atggaaaagt tcactacatt gtaatggagt
     4621 ttgtagatgg gaagaatcta aaagagatta tacaagatga aggtatatta gatgaatata
     4681 ctgcattaga tataacaaaa caaatagcta tggcacttag tgcagcgcac aaaaagggga
     4741 ttatacatag agatataaaa cctcataata ttctcatatc aaatgaagga agagtagtaa
     4801 aagtggctga ttttggtata gccaaagctg tttcaaattc aacaatgact aatattggta
     4861 gtataatagg ttcagtgcat tatttttcac cagagcaagc taaaggtaaa tttgtaacta
     4921 ataatgctga cctgtattct ctaggtatag ttttatatga aatgttgata ggaaaagttc
     4981 catttagggg agatagtcca atttctatag cacttcaaca cattaatgat gatatagact
     5041 ttacatcaga agaaaaagtt aggattcctc aaagtgtaag aactacaata aaaaagctta
     5101 cagaaaaatc tagtgcagat agatatcaaa ctgctgaaga attgatagaa gatatagagt
     5161 atatagaaaa aaatattgat ttagatttta taaaagaata tgatgatttt gcaactaaga
     5221 aaattgatga aaaggaaatt aataaagttg taaatccaac acttgcaaaa ccagctcctg
     5281 aaaaagtggt taagcctgtt gaagtagctg atttggatga tgatgaagat tactatgatg
     5341 atttttatga agaagatgat gatgaagatg aagaagagga agagataatg agagctaaaa
     5401 agaatcagag acctaagagt actccaagta agagaactaa gaaaaagaag aaaaaacagg
     5461 aaagcccaaa atctagaaga agattaaaag taattgcggc tgttttgata ctgatattgt
     5521 gtgctcaagt attcttagca tataagtttt tatttgctgg agggtttggc aataaatctt
     5581 tgactgttcc taatcttgta aatatgacat tagaagaagc tcaaagtgct gttgaaaaag
     5641 aaggattata cctgagtgta aaaagtgaag agtataatag tgaagttgat gaaaattgta
     5701 taatatcaca gacacctgaa ggtggaagta caaatgtcaa aaagggtgat actataaatg
     5761 ttgtcgtaag taaaggttca agtcaggcat ctgttcctaa tgtagtggga cttactttga
     5821 gtaatgcaaa acaacttatt gaagaaaata acttaaaagt gggaactgtt aagtatgaat
     5881 acagctcaat atataaagaa ggtacagttt taagtcagag tccaggtgct ggctcatcaa
     5941 gagctcagga aggtgatgag gtaaacctat atgtaagcaa aggttcagaa aaatcaaata
     6001 cacagactcc aacagtacca gataaaaaac caacaacacc agagaataat acaccaacag
     6061 aacctggttc aaactcagga aacagtggtg gaaattcagg aggctcaaat tcaggaaata
     6121 gtggtggaaa ctcaggcaat aatggaagta actcaggagg ttcaaactca ggaaatagtg
     6181 gtggaaactc aggaggttca aactcaggag ataatggagg aagttcagga ggctcaaact
     6241 caggagacaa tggaggaagt tcaggaggtt caaactcagg agacaatgga gatagttcag
     6301 gaggctcaaa ctcaggagat aatggaggaa cttcatcagg aactggcgca aacattggta
     6361 aaacagaata aatattaata aaataagagc tgtcctttta tagaaaatat tttctttaaa
     6421 gggcagtttg ttttgtataa aatactgaat tggttataat ataaggagaa aagtatttat
     6481 gaagaagaaa acagttttaa ttacaggagg agctagaggg attggtaaag ctatgtctaa
     6541 agcttttgca aaagaaggtt acaatgtatt agtaaatttt aataagtcag aaaatgaagc
     6601 taaagagttg tacactatat taaatgaaaa aaatttttct gtaaaacttt ttaaggctaa
     6661 tatttcaaat agagaagatg tagaggatat ggtagattat tgtataaaag aatttggagg
     6721 attggatgta cttgtaaaca atgctggtgt aagtcaagat aagttgttta cagatataac
     6781 tgatgaagat tgggataata tgatgaatat taatttaaaa ggaagctttt attgttcaca
     6841 agttgcttta aaatacatga tatcagaaaa aaaaggaaat ataattaata tatcttcaat
     6901 ctggggtata tcaggtgcct cttgtgaagt gcattattct ataacaaaag caggcataat
     6961 aggtatgaca aaggcattag ctaaagaagt aggaccatca aatattagag taaatagcat
     7021 agctccaggt gtcattaata ctgacatgtt atctggatat aatgaggaag atatagacgc
     7081 tcttgtggaa gaaacccctc taatgaggct aggaactcca gaagatatag caaattgtgc
     7141 tattttcttg gcatcagaca aatcaaattt tatcactggt caggtaatta gtccaaacgg
     7201 aggatttgtt atttaaaaaa aatgtggtat tatataaaaa taggaaagaa taacacgata
     7261 tactactcat aaacagtatg tgtttatata aattttataa agggaggcat ttgatgctag
     7321 aaggaaaaat tataaagggg attagtggat tttattatgt agatacttat aatggtattt
     7381 atgaatgtaa ggctagaggg atatttagaa agcagaaaat aacacctttg gttggtgata
     7441 gagtcaaaat aagcattgtt gatgaagatg aaaaaaaggg tatcttagaa gaaatcgaca
     7501 gtagagatac agaacttata agacctccta ttgcaaatgt agataaagca ttaatagtat
     7561 tcgcaataaa aaatcctaaa ccaaacttat ctcttttaga tagatttata gttttggcag
     7621 aaaaagaaaa tttagaaact gtaataatcc ttacaaaagc agatttagat gataatgata
     7681 cattagaaac tgttaaaaat atatatgaac ttagtggcta taaagtcatt cctgtaagta
     7741 atattactaa gttaaatata gataaagtaa aagaagaatt aaaagaaaat gtagtagtgt
     7801 ttgcaggacc atctggagtt ggaaaatcaa gcttattaaa tgaaatagat gaaaatttta
     7861 aacttcaaac aggagttgta agtgataaaa taaaaagggg aaaacatact actcgtcacg
     7921 ctgaactttt aaaattagaa tttggtggaa tggttgcaga tacaccaggt tttagttccc
     7981 ttgtccttga agatatagag gaagttgaac ttaaagatta ttttatagaa tttgataaat
     8041 ttaatgattg taagtttggt tcaaagtgta ttcatgaaaa tgaacctaat tgtgctataa
     8101 aagaagctgt tacaaatgga gaaatatcta aagaaagata tgatagttat atacagctgc
     8161 tacatgaaat aagacaaaat aatagtagga ggtattagaa acatgattaa attagcacca
     8221 tcaatattat cagcagattt tgcaaaatta ttagaagatg taagaaaagt agaaagtgca
     8281 ggatgtgaat atcttcatat cgatgtaatg gatggacact ttgtaccaaa cattacatta
     8341 ggaccacttg tagtcaaaag tttaaagaaa gaaaatataa atatggtatt tgatgctcat
     8401 cttatgatag aaaatccaga ccagtatatt gaagaatttg taaaagcagg atgtgatata
     8461 ataactgttc accaggaggc ttgtgttcat ctacatagaa ctatacaaaa tataaaatca
     8521 catggaataa aagctggtgt agtacttaat ccagctacac cagtagatac gattaaacat
     8581 gtactacctg atttagatat ggtattatta atgtctgtta accctggatt tggaggacaa
     8641 tcatttattc cttgtgtttt agataagatt aaagagttaa aagctattat agatagtcaa
     8701 ggattaaaca ttgatataga agttgatggt ggtataagcc ctaaaaacgt agccgaagtt
     8761 gtacaagcag gagctaatat aatagtagca ggttctgcaa tatttggaag tgatgacatt
     8821 caagaaacag ttaatttatt tagaaagaat gcttctcttg aagaattagt ttaaatttaa
     8881 taatgaaaat ctgtattgtt ttaaatggcg aaattgaaga ttacgaagtt acaagagata
     8941 ttataattaa ggaatgttat gactgcataa tatgtgcaga tggaggggca aatcacactt
     9001 ataaaatgga aataatgcca gattatatat taggagattt agattcagta gaagaagaaa
     9061 agataaattt ctataaaaat aaaggtgtta aatttgaaaa atttccatct aaaaaagatg
     9121 agactgatac agagctttgt ctttttttag ctaagacact taaagcaaat catattgatt
     9181 tttttggtgc tttaggggga aggattgacc acactcttgc aaatataaag ctattatatt
     9241 atttaaaaga agatggtata tattctagga tactttctga taaagaagag atgtatatag
     9301 ttgaaaatga ggaaatttca ttgtatggaa acccagggga cactatttct gtaatagcta
     9361 taaatggtga tgctaagggt gtaacactta ctggcttaga ataccctctc gataattatt
     9421 atatgaaata ctctgtacct attggaatat caaatgttat gttgagtaat tcttgtaaga
     9481 ttaaagtaga gcaaggctgt gtacttgtag ttagaaattt ataaaatttg attttgacaa
     9541 ttggttttat attgtagagc agatacaatc aagtaaaata tatttatgat tgtatctgct
     9601 ttttagtata taattataaa tatataataa actttgacat aagggggatt ttttgtggta
     9661 gatagtatta tttttgattt agatggaaca ttgtgggatt caactgaaga agtatgcaaa
     9721 gtctggcagg atgttttaag taaacataag gaaatagaat taagtgtcac aaaagattta
     9781 tttagaagtc ttatggggct ttcttttgag gaaatagcaa atcgtttatt tccaaattta
     9841 agtgaagaag aaagaatgaa tttattgaat gaatgttctt tgaaagaatg tgactattta
     9901 tctgaaaatg gagctaaatt atttgatgat atagaagata ctcttaaatt actatctaaa
     9961 aaatataaat tatttatagt aagtaattgt caagctggat atatagaatc ttttttaaaa
    10021 gcacataaac tagaacagta ttttatagat tttgaatgtc ctggtaatac tggtttgcat
    10081 aaaggagaaa ataataaact tataattgaa agaaataaat taataaatcc aatctatgtt
    10141 ggtgatacgc aaggtgatgc aaatagtgct aagtttgtag gtataccttt tatatatgcc
    10201 aaatatgggt ttgggaatgt agatgtatat gattatttta tagactcatt taaagatttg
    10261 ttagaacatg atatattaaa ataagatgag tttaaaaatt atattaaaat aataaaaaag
    10321 ttatgggggg atttaaaaat ggaattacaa gatactattt ttaaaagaca aagtgtaaga
    10381 aagtttaaaa atcaagatgt atcagatgaa gatattttaa aaatgataaa agcagcaggt
    10441 gcagcaccat caggcaagaa tattcaaaac tggcattttg ttgttataaa acgccgtgat
    10501 ttaatggaaa agatagcaga tgtgataact aaaaagcaac aagaaatact tgcagaaatg
    10561 gataaagtat cagtggataa ggctaataga tttagaaaat ttgttaaaaa ctttacacta
    10621 ttttatttaa aagctccagt tttagtatta gtttttacaa aggtatataa cccatcagga
    10681 tattatgaat tagagcttat agatgcacct aaggagacta tagataaatt atttataaga
    10741 aatccaggta tgcagagtct aggtgctgca attgaaaact ttacactatc agcaattgaa
    10801 cttggatatg gttcttgttg gttgacaagt caaaactatg cagcagatga gatagaagct
    10861 gttttagaag cagaaactgg ttttgaaaag ggagagtatt ttttaggtgc aatgttagct
    10921 cttggagtgc cagaggacaa tttgaagagt ccatctaaaa aaccagtaga agaaatatgt
    10981 acatttataa aataaaagtc aaggacattt taaaatcgaa gtaaattaaa aacaaaataa
    11041 attaaaaata aatatttaaa aaattaaaaa gtaggtttta ttgatattaa ttttatattt
    11101 caaatca
//
LOCUS       DAENCV010000120        10967 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAENCV010000120 DAENCV010000000
VERSION     DAENCV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.122"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            73..3552
                     /locus_tag="KQD82_003061"
     CDS             73..3552
                     /locus_tag="KQD82_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG4381516.1"
                     /translation="MGKIEPNQEDRIYEDLYQGNSAEGKVCEWIEEAINDDCYVGQHR
                     FKIETRECDIAMVIPNRGILIIEVKGYTVEMIDHMSSNFIIHVKKEYENERFKTGERH
                     PAEQANDYVFTMMRDFSGFLKRNNICLPICKIGRMAAMPNMTDKEYEELYLDYCYSTK
                     LVITKDWFKNKNIFYHRFINALNIIFNSISSEVDFDDSYYNIARRYILLGERKKYVDL
                     CKEQLKREEELGIDFIIDNTNEEVDQLCKSNEVNDEEMELIKFSSVLQEELTESHKLS
                     KIIPYSKLRIVKTGCEIEPLLEICCNERKEGIKQFLILVNFTRDEKEKILLEYEKRFL
                     NKYKENELLFLFNMYFIDLSNDDTSIDNLFSNHDNIEIINGERIEEIVKIKPDNFIVQ
                     EFTPKDLLDQALWNILCIADNFTSFNFDQYIVEHSPLRQNIVVTASAGTGKTYTMVDR
                     VSFVAHMEIIYRNSKESISDLISMITFTNNSTDEMKERLMKHFMQYFELTHNKVYMDL
                     LEQLGRMKIKTIDSFTKYILSKFAHLLGLSNSFSLKSGKFELENILRKYINDEITKEI
                     IDDFHNINIYEIANHILALRSFLINRKIQFDKIKHASIVGPDVGKGTIRMKNFIESVL
                     ISTNSEYDSFNCSQNFISMNELVIQLEKVKNKLIKTSCVNNTKENYEKYLFIDEFQDT
                     DNKQIQLIRFFADYFNIALFVVGDEKQCIYGFRGAERTSFLELTCVDTNFQQRNWISC
                     FLRKNYRTEEPLVKKINEVFSKMPNDFFPYQNKDKLVAQKGIGRIYSGCYEQFKYSEN
                     NLIELVDNCVKRMKKNNEDGQIAILTRNNYEVDQIRALFGENKEYEIKFDNGGLFYNT
                     DAVIDFYKLVEFLMDTEDGAKRYSILETPYSDFRIKESKIKLYQAYEKMDVETFEIIR
                     SGNNHVSLRKYYDVLYNYGKKHPNLKNSPSLKILRMIVNEIKPWEHYVPERYKTVDFN
                     DICNQNKLIEARNEYRQNLFQLFQKLIDDNKTDYLTLNKIHQILTIGIFAMQDIDIDL
                     PKNNFFGEKISVLCTTVHKSKGLQYHTVIMPFTMNDITESRGSTQFIYREDTNEIYMQ
                     LKEADGKCLIYESTNFDCENNKKNETIQSEELRILYVALTRSKKFFYFMVDEERFEKN
                     NTSKKNCWAHYLM"
     gene            3569..6805
                     /locus_tag="KQD82_003062"
     CDS             3569..6805
                     /locus_tag="KQD82_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381517.1"
                     /translation="MKIYTYKNSVQLKDAIFWNEIEGAPHFCSSEVLAIGMRKFYGRN
                     TFPCISTIDLLIKELYPNWLKSHSDIERYKDISKLINELNDSSTEGLRQAFVKNKKSI
                     LDSCKYLIETEDLLLKDYYNKNIFNKFSAINDIQEVFKEKILKRLLEQIYIDGNKNYW
                     KPEWIDTLWVKSKLSEVIHKCVIKEISDIVKNTEEKSLNHNYDEGHIFEIFDELFDQH
                     GLFNISDKTLEYWKFNSKDDLLDKIGYSSKELEYVRNLATTECKSYKTIVIHGVYHLN
                     PIHLRLLRDLERDHNVIVLNCYNKEFNHIYEFWNDIYDHLIKIFQIPKNNIKIDSHDS
                     ANHREVGSIFAEVVNGVINKQSNFFKISRNEFGPDLNLNSKDIDELFNHNSSQDIRYI
                     LNKSEAIIQNYEDEDEREYQKILIDTSKELQKELCHRSKILIKYDSTMQFVNKVCNIF
                     DETKNKKGIRSVGRMKEQFYGSKGTEMNKIFQVFYPDEFKVKPFLSYPIGQFIYAIHN
                     MWDLKNHKLILNSRDLAECLNLDVWHDIHPLEVFESLRFYIGIDKEPNGMNVKDFIDR
                     IKKLIYFKERNENKMKNLDNLSYLNLTIDQYNRFIQVIENINEIANKIFCNKSQTSKK
                     HFMDLLEILNDEKAWKPLLEEEKRLMDEIKNQIIHIDDETNKTDLNTIKDTMAYYLDS
                     KEGTDINWLVRDFEQLEGDILSEAANYKNNTLRQKCYHLTMLSNENMINCDIDNLPWP
                     LSDDFIISKNISSFIHTICINYAKYKRCLLFQGLYYLINNPIISLKLSYIENQGEYKH
                     TPYYILNMFMNIGKDFDQSNCRNIESCTSPINKEPKKRFKIDKKWHKDAIESFSCCPY
                     KFMYGYVINDAAKHYKDELQVKMYLGEIIKYYISIAWKKILQYHKKLLLDQKFNLLKK
                     WTAGERLDCLEKSLPETLEPIYGNYFIKSEINKIIVDNLEYLKNKRIDNKRVYFTEEC
                     KIYEVDRELYHCWIGKISNHNLYVTNKNKIINIRNRLNLLNANEYINDYILGINDGWY
                     FKKDNIVVEGGYKVSEHACRYCSYNKICCYQYRIKPIHLLHSKEDLID"
     gene            complement(6917..7300)
                     /locus_tag="KQD82_003063"
     CDS             complement(6917..7300)
                     /locus_tag="KQD82_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HBG4381518.1"
                     /translation="MRFKLKTSTQTKEIFEELGSRMNLKPFALCKLAISMSLSYSESI
                     ESYENIDNNGLELNRQTITNQYDTLFKTLFEQHIGRHLSDNEYFPNQVKLHIDRGAIM
                     LKNRYNYSSNLEHFITQIIQEDNNL"
     gene            complement(7303..9462)
                     /locus_tag="KQD82_003064"
     CDS             complement(7303..9462)
                     /locus_tag="KQD82_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381519.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSDKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPMAYKYQGFNASYISQVKSNINNNSLINSKIDTLVSVDIEISEGTEEN
                     IYTLIRQWTFKDEKLNETFLVYKNFSTTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFVGKNSNIHLKESLLSLCNFDTFDILKKVLISTNRSNKNVSDEINIVKDSYL
                     NLEMEINTLNSQEEIFSYELQKISNEIEKLQQEQLEVKKEFRKKGGILAEERETLNSR
                     IAELEGKRFIINQNIKEFCNDILPFLIVKKQISNLEKQLKTENDILLYSRLKDKLNIE
                     YIKNLLSDKIMPSALDEIALTVSEALTEDIKPDSYEENFKSIHNLSDDESRKLLSLIN
                     TILNFDNNSIINSFEEHRSISNELADIRHKLNTSLKDESLNTYINDMSNLSSKIDSLL
                     GSKNLLVTSLDKLKLEIAETKSRRDKSKAKYIYLLQHNNIVDISTNLILMLDEIVTTL
                     TETKIKLIQDNFMYIFKKIIQKDNFISHIDINVNFDISLYINKIYNSLELENLAKNIG
                     IEGMEKKLGKLFFDDLFNLYSVEDKYELFNVIRNNTQSTFLNLRTKVDIDEFSSGEKQ
                     IYVLCLYWALLKSSGIEIPFIIDTPYARIDDTHRNSITSEYFSTISHQIIILSTNTEI
                     DEKLYKIIKPKLSREYLLEYDSKNKKTIQNKGYFFEV"
     gene            complement(9449..10915)
                     /gene="dndC"
                     /locus_tag="KQD82_003065"
     CDS             complement(9449..10915)
                     /gene="dndC"
                     /locus_tag="KQD82_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBG4381520.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGKI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDEIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLIIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEEGNKELKYENK"
BASE COUNT         4184 a         1233 c         1568 g         3982 t
ORIGIN      
        1 aagataatag tataagcaat taaggatgca atattaaatt cttaataaac ttatatctag
       61 ggaggatatc aaatgggaaa gattgaacct aatcaagagg accgtatata tgaagattta
      121 taccagggaa atagcgctga aggtaaggta tgtgaatgga tagaagaagc tattaatgat
      181 gattgctatg taggacaaca tagatttaag attgagacaa gagaatgtga tattgctatg
      241 gttataccaa atagaggcat attaataata gaagtaaagg gctatactgt agaaatgatt
      301 gatcatatgt caagtaattt tattatacat gtaaagaaag agtatgaaaa tgaaagattt
      361 aaaactggtg aaaggcatcc agcagaacaa gcaaatgatt atgtatttac aatgatgaga
      421 gatttttcag gttttttaaa aaggaataac atatgtttac caatatgtaa aataggacga
      481 atggcagcaa tgcctaatat gacagataaa gagtatgagg aattatactt ggattattgt
      541 tattcaacaa aacttgtaat cacaaaagat tggtttaaga ataaaaatat attttaccat
      601 cgttttataa atgcacttaa tataattttt aattctataa gcagtgaagt agatttcgat
      661 gatagttatt ataatattgc gagaaggtat attcttttag gtgaacgtaa aaaatatgta
      721 gatctatgta aagagcaact taagcgagaa gaagaattgg gtattgattt tataatagac
      781 aatactaatg aagaagtaga tcaactttgc aaatcgaatg aagttaatga tgaagaaatg
      841 gaattgataa aattttcttc tgttttacaa gaagaattaa cggaatcaca taaactatct
      901 aaaattattc cttattctaa attacgtata gttaagacag gttgtgaaat agaaccactt
      961 ttggagattt gttgtaatga acgtaaagaa ggaataaagc agtttctaat attagttaat
     1021 tttacaagag atgagaaaga aaaaatattg ttagaatatg aaaaaagatt tttaaataag
     1081 tataaggaaa atgaactgtt atttttattc aatatgtatt ttatagattt atctaatgat
     1141 gatacaagca ttgacaatct atttagtaat catgataata ttgaaattat taatggagag
     1201 agaatagaag agattgtcaa aataaaacct gacaatttta ttgtacaaga atttacacca
     1261 aaagatctgt tagatcaagc tttgtggaat atattatgta ttgcagataa ttttacttca
     1321 tttaattttg atcagtatat agttgaacat tcacctcttc gtcaaaacat tgttgtaact
     1381 gcaagtgctg gtacaggaaa gacatatact atggttgatc gagtttcatt tgttgcacat
     1441 atggagatta tttatagaaa ctctaaggaa tctattagtg atctaatctc tatgataaca
     1501 tttactaata attcaacaga tgagatgaag gaacgattaa tgaaacattt tatgcagtac
     1561 tttgagttaa cacataataa agtatatatg gatttattag aacaattagg tagaatgaaa
     1621 attaaaacaa tagattcatt tacaaaatat attttatcta aatttgctca tttattaggt
     1681 ctttctaata gtttctcatt aaaaagcgga aagtttgaat tagagaatat tttaagaaaa
     1741 tatattaatg atgagataac taaggaaatt atagatgatt tccataatat aaatatttat
     1801 gaaattgcta atcatattct tgctttgcgt tcgtttctta ttaatcgaaa gattcaattt
     1861 gataaaataa aacatgcttc tattgtggga cctgatgtag ggaagggaac tattcgtatg
     1921 aaaaatttta tagagtctgt acttatttct acaaatagtg aatatgatag ttttaattgt
     1981 agtcaaaact ttatttcgat gaatgaatta gttattcaat tagagaaggt caaaaataaa
     2041 ttgataaaaa ctagttgtgt taataatact aaagaaaatt atgaaaaata cttgtttata
     2101 gatgaatttc aagatacaga taataaacag atacaattaa taagattttt tgcggactat
     2161 ttcaatattg cactttttgt tgttggagat gaaaaacagt gtatttatgg attcagagga
     2221 gcagaaagaa cttcattttt agaacttaca tgtgtagata ctaactttca acaaagaaat
     2281 tggatatcat gttttctaag aaaaaattat agaacagagg aacccttagt aaaaaaaata
     2341 aatgaagtgt tttctaaaat gccaaatgat tttttccctt atcaaaataa agataaatta
     2401 gtagctcaaa aaggaattgg aagaatttat tcagggtgtt atgaacaatt taaatatagt
     2461 gaaaataact taatagaact cgtagataat tgtgttaaaa gaatgaaaaa aaataatgaa
     2521 gatggacaaa ttgctatttt aacaagaaat aattatgagg ttgatcaaat acgtgctttg
     2581 tttggagaga ataaagaata tgaaattaaa tttgataatg gtggattatt ttataatact
     2641 gatgctgtta ttgattttta taaattagtt gagtttttga tggatacaga agatggtgct
     2701 aaacgatata gtattttaga aactccttat agtgatttca gaattaaaga atcgaaaata
     2761 aagctatatc aagcttatga aaaaatggat gttgaaacct ttgaaataat taggtctgga
     2821 aataatcatg tatctttacg aaaatattat gatgtattat ataattatgg gaaaaaacat
     2881 ccaaatttaa agaatagccc aagtttaaag atccttcgaa tgattgttaa tgaaattaaa
     2941 ccatgggaac actatgtacc agaaagatat aaaactgttg attttaatga tatatgtaac
     3001 cagaataagc tcattgaagc tagaaatgag tatcgtcaaa atttatttca gttatttcaa
     3061 aaattaatag atgataataa aacagactat ttaacactta ataagataca tcaaatttta
     3121 actattggta tttttgcaat gcaagatatt gatattgatt tgcctaaaaa taattttttt
     3181 ggagaaaaaa taagtgtatt atgtacaaca gtacataaat ctaaaggtct tcagtatcat
     3241 acagtaatca tgccatttac aatgaatgat ataacagagt caagaggatc aacacaattt
     3301 atttatagag aagatacaaa tgagatctat atgcaattaa aagaagctga tggaaagtgt
     3361 ctaatttatg aatcaaccaa ttttgattgt gaaaataata agaaaaatga aactatacaa
     3421 tcagaagaat taagaatttt atatgttgca ttaacacgtt caaaaaaatt tttttacttt
     3481 atggtggatg aagaaagatt tgaaaaaaat aatacttcta aaaaaaattg ttgggcacat
     3541 tatctaatgt aaggtggagg aaaataatat gaaaatctat acatataaaa attctgtaca
     3601 attgaaagat gcaatcttct ggaatgaaat agaaggagca cctcattttt gttcctctga
     3661 agtactagca ataggaatga gaaaatttta tggtagaaat acttttccat gcatttctac
     3721 tatagattta ttgataaaag aactttatcc aaattggctt aaatctcata gtgatataga
     3781 gcgatataaa gatatatcta aattaattaa tgaacttaat gattcaagta cagagggttt
     3841 aagacaagct tttgtaaaaa ataaaaaatc tatactagat tcttgtaaat atttgattga
     3901 aactgaagat ttgctcttaa aagattatta caataagaac atatttaata aattttcagc
     3961 aattaatgat attcaagaag tttttaaaga aaagatactg aaaagacttt tagaacaaat
     4021 atacattgat ggaaacaaaa attattggaa acctgagtgg atagatacat tatgggttaa
     4081 atcaaagtta agtgaagtaa ttcataaatg tgtgataaaa gaaattagtg atattgttaa
     4141 gaatacagaa gaaaagagtt taaatcataa ttatgatgaa ggtcatattt ttgaaatatt
     4201 tgatgaactg tttgaccagc atggattatt taatatatca gataagactt tagaatattg
     4261 gaaatttaat tctaaagatg atttgcttga taaaattgga tactcatcaa aagaactaga
     4321 gtatgttcgt aatctagcta ctactgaatg taagtcatat aagactatag taattcatgg
     4381 tgtctatcat ttaaatccaa ttcacttaag attgttaaga gatttagaaa gagatcataa
     4441 tgtaattgta ttaaattgtt ataataaaga attcaatcat atttatgaat tttggaatga
     4501 tatatatgat catctaatta aaatatttca aattccaaag aataatataa aaattgatag
     4561 tcatgattct gctaatcaca gagaagttgg tagtatattt gctgaagttg taaatggtgt
     4621 aatcaataaa caaagtaatt tctttaagat aagcagaaat gaatttggcc cagacttaaa
     4681 tttaaattct aaggatattg atgaactttt taatcataat agtagtcagg atattagata
     4741 tatattaaat aaaagtgaag ctattattca aaactatgag gatgaagatg aaagagagta
     4801 tcagaagata cttatagata catcaaaaga acttcaaaaa gaactatgtc atagaagtaa
     4861 gattttaata aaatatgatt ctacaatgca atttgtcaat aaagtgtgta atatatttga
     4921 tgaaacaaag aataaaaaag ggatacgttc agtcggaagg atgaaggagc aattttatgg
     4981 ttctaaaggt acagaaatga ataaaatatt tcaagtattt tatccagatg aatttaaagt
     5041 taagcctttt cttagttatc caattggaca atttatttat gcaattcata atatgtggga
     5101 tttgaagaat cataaactta tattaaatag tagagattta gctgaatgtt taaatttaga
     5161 tgtatggcat gatattcatc cattagaggt atttgaaagt ttacgttttt atattggtat
     5221 tgataaagag cctaatggta tgaatgtaaa agattttatt gatcgaataa aaaaactaat
     5281 atattttaaa gaaagaaacg aaaataaaat gaagaattta gataatctta gttatcttaa
     5341 tttaactata gaccaatata atagatttat tcaagtaatt gaaaacatta acgaaattgc
     5401 aaataagata ttttgtaata aatcacaaac aagtaaaaag cattttatgg atttactaga
     5461 aatattaaat gatgaaaaag cttggaaacc tctattagaa gaagaaaaaa gattaatgga
     5521 tgaaattaaa aatcaaatta ttcatattga tgatgaaact aataagactg acttaaacac
     5581 aataaaggat actatggcat attatttaga tagcaaagaa ggtactgata ttaattggtt
     5641 ggtgcgtgat tttgagcaat tagaaggcga tattctttca gaagcagcaa attataaaaa
     5701 taatactttg agacaaaaat gctaccattt aactatgttg tcaaatgaaa atatgattaa
     5761 ttgtgatata gataatttac catggccatt aagtgatgat tttattatat caaaaaatat
     5821 ttcttccttt atacatacaa tttgtataaa ctatgcaaaa tataaacgtt gtctattgtt
     5881 tcaaggatta tattatttga taaataatcc aattatttca cttaagttaa gttatattga
     5941 aaatcaagga gagtataaac atacaccata ctatatttta aacatgttta tgaatatagg
     6001 aaaggatttt gatcaatcaa attgcagaaa tattgaaagt tgcacttctc caattaataa
     6061 agagccaaaa aaacgtttta aaatagataa aaaatggcat aaagatgcta tagagtcatt
     6121 tagttgttgc ccatataagt ttatgtatgg atatgttatt aatgatgctg caaaacatta
     6181 taaagatgag ttacaagtga agatgtattt aggagaaatt ataaaatatt atattagtat
     6241 tgcgtggaaa aaaattttac aataccataa aaaactatta ttggatcaaa agtttaattt
     6301 actcaaaaaa tggactgctg gtgaaagact agattgtctt gaaaaatctt taccagagac
     6361 actagaacct atttatggta attatttcat caaatctgag attaataaaa ttatagtaga
     6421 taacttagaa tatttaaaaa ataaaagaat tgataataaa agagtatatt ttacagagga
     6481 gtgtaaaata tatgaagttg atagagaact atatcattgt tggataggta aaatatcaaa
     6541 tcataattta tatgtaacaa ataaaaataa aataattaat attagaaatc gtctaaactt
     6601 actaaatgca aatgaatata taaatgacta tattcttgga attaatgatg gatggtattt
     6661 taaaaaagat aatattgtag ttgagggagg atataaagta tctgaacatg cttgtagata
     6721 ttgctcatat aataaaatat gttgttatca gtataggata aaacctatac acttgctaca
     6781 ttcaaaagaa gatttaattg attaataatt agataaactt taagaactat atcaatagga
     6841 tagacggttt tatttatttt ctatcctatt gattaaatct aaaagtttta gttttttagt
     6901 tacatattga atcatattat agattgttat cttcttgtat tatttgagtg ataaaatgtt
     6961 ctaaattact ggaataattg tatctgtttt taagcataat tgcaccccta tctatatgaa
     7021 gctttacttg gttaggaaaa tattcattat cacttaaatg tctacctata tgctgttcaa
     7081 ataaagtttt aaataaagta tcatattgat tggttatggt ttgtctatta agctcaagac
     7141 cattattatc aatgttctca taactttcaa ttgattcact ataacttaac gacattgaaa
     7201 tagccagttt gcaaagagca aatggtttca aattcattct agatcctaac tcttcaaata
     7261 tttcttttgt ttgtgttgat gttttaagtt taaatctcat aattacacct caaaaaagta
     7321 tcctttattt tgtatagttt ttttgttttt gctatcatat tcaagcaagt attctctact
     7381 taacttaggt tttattattt tatataattt ctcatctatt tcagtattag tagatagtat
     7441 tataatttga tgacttattg tagaaaaata ttcactagta atgctatttc tatgagtatc
     7501 atctattcta gcataaggag tgtctataat aaaggggatt tctatacctg aggatttaag
     7561 taaagcccaa tataaacaaa gaacatatat ttgtttctcg ccactagaga attcatctat
     7621 gtcaacttta gttcttagat ttaaaaatgt agattgagta ttatttctta taacattaaa
     7681 tagctcatat ttatcttcta cgctatacaa attaaataaa tcatcaaaaa ataattttcc
     7741 caactttttc tccattcctt caattcctat gttttttgct aaattttcaa gctctaaaga
     7801 gttatatatt ttatttatat ataaagaaat atcaaagttg acattaatat ctatgtggct
     7861 aataaaattg tctttttgaa ttatcttttt aaatatatac ataaagttat cctgaattag
     7921 tttaatttta gtttctgtta atgttgttac tatttcgtcc aacataagta taagattagt
     7981 agatatatca acaatattat tatgttgaag taaatatatg tatttagcct tagatttatc
     8041 cctcctactt ttagtttcag ctatttcaag ttttaattta tctaaagaag taactagtaa
     8101 attctttgat cctaataaag aatcaatttt gcttgataaa ttggacatat cattaatata
     8161 tgtatttaaa gattcatctt taaggctagt atttaacttg tgtcttatat cagctaattc
     8221 atttgatata cttctatgtt cttcaaaaga attaattata gaattattat caaaatttaa
     8281 aatagtgttt atcagagata ataattttct actctcatca tcagaaaggt tatggataga
     8341 tttaaagttt tcttcataac tatctggctt tatatcttct gttaaagctt cagaaacagt
     8401 taaagctatt tcatctaagg cagaaggcat gatcttatct gaaagtagat tttttatata
     8461 ctcaatattt aatttatcct taaggcgaga atatagtagg atatcattct ctgttttaag
     8521 ttgtttttct aaatttgata tctgtttttt tacaattaaa aagggaagta tatcattaca
     8581 aaactctttt atattttgat taattataaa tctctttcct tctaattcag caattctaga
     8641 attaagggtt tctctttctt ctgctaggat tccacccttt ttcctgaatt ctttttttac
     8701 ttctaattgt tcttgttgta acttctcaat ttcatttgaa attttttgta attcataaga
     8761 aaaaatttct tcttgagagt ttaaagtatt gatttccatc tctaaattta aatagctatc
     8821 ttttacaata ttaatttcat ctgaaacatt cttattagat ctattagttg aaatcaacac
     8881 tttttttaaa atatcaaatg tatcaaaatt acaaagagat aataaagatt cttttaaatg
     8941 tatatttgaa tttttaccta caaaaaaatc atacaaatgt tctccatcaa aaaagaaaaa
     9001 atcaaagatt ttaggagaaa taatagatgt aatataattc tcaaaatagt taagttcgtc
     9061 agcatttaat ggtgtagttg aaaaattttt ataaactaaa aatgtctcat ttaatttttc
     9121 atccttgaaa gtccattgtc taattaaagt atagatattt tcttcagtgc cttcactgat
     9181 ttctatatct actgatacta aagtatcaat cttactattt attagagaat tattgtttat
     9241 gttagattta acttgagata tatatgaagc gttgaaacct tgatatttat aagccatagg
     9301 tccatatata catagcttta tagcctcaaa tattgtagat ttaccagcac catttttacc
     9361 acctatcaat ataatatttt tgtcactagt agtatttaga ttgaatgttg tttcttcttc
     9421 ataagatctg aaatttttta atacaagttt atttattttc atattttaac tccttgttgc
     9481 cttcttcttc aatcttttca ataatatctt gatgaagcca ctgttgattt aatattcttt
     9541 ctatggaagt ttttaattta gtattattac taaaatattt atatttattt gtatctatga
     9601 ttaatttatt aaataaatca atatttatat catactcact acatttttca gatataatat
     9661 ttaaagtaag gtcatcaaat actggttttt tatattcaga ccatggtagg tttttaccta
     9721 caacttcctt atatattttt gataatatat taccagttaa gtcttcttcg ttatcccaaa
     9781 tctcatctat agctttaagt tctcctaatt gtataagttg taaatcagga taaaattttt
     9841 gcatttcaag ttgagtttct aaaagttttc ttaaaatagt ttgccttgct tcaaatgtaa
     9901 atggtccaaa catgatttta ccatcttttt taataacaga gccatttctt ttatgttttt
     9961 tacgattttt aggtttatct ctctcagaaa ttagccaact tctgaattcc aaaagtggct
    10021 ttaattcatc atgaccagat tcaataaatc cgtttaatga tttgtcttct tttacaacag
    10081 tacaaatcca acatccaaac ctactatttc cacagctact tttagcacta gcttcatctg
    10141 ctataaatgg gcattcatct gagtcaccag aacctttcat atacatctga aataattcca
    10201 tagcatcagt tccccatgag gtagatggag atggtccaaa ttcaccacca aaatctatga
    10261 aattatcatt ttgatgatag agtacatccc aaacatctgc tgtactaaaa ttgactatag
    10321 gggtatacac ataagctaac ttatttttac ctcttaaggt ttcatgtggg gtaagaaggt
    10381 aaccctctat ctctcttttt tcgattctcg attttctagc agaactttct tctttacgaa
    10441 cgccaagcag tacaataaca tctttttgtt caagtttttc ttttataaat ttatttgatg
    10501 gttttatctt aagcctttca gtacaccaac gatatttaat agactttgga gtagggtaac
    10561 ctctaccaat tatattggcc caaaaactat cattaagttc tgggtataca atctgtgcac
    10621 ttataggtaa atcatgttca gtagcacttt catttatctt gtctatatta ttttttagat
    10681 aatcaagtat tattggattt tctataagtg tatcagatga gattacataa atctctttat
    10741 gtcttttttc tttgggtagt cttttaagca ttctaaatgt aagttcaact acacaagaac
    10801 tatcttttcc agaactatat ccgattatcc aaggtctatc atcattaaga tatacggcct
    10861 gcatttcatc atataaattt tctaaattgt tgttatctag gtaattaaca gccatatatt
    10921 aagtatcctt tctataagag tatagcgaag atctttacat aatactc
//
LOCUS       DAENCV010000121        10932 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAENCV010000121 DAENCV010000000
VERSION     DAENCV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.123"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(339..1388)
                     /locus_tag="KQD82_003066"
     CDS             complement(339..1388)
                     /locus_tag="KQD82_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381521.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            complement(1618..2703)
                     /locus_tag="KQD82_003067"
     CDS             complement(1618..2703)
                     /locus_tag="KQD82_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG4381522.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2739..4052)
                     /locus_tag="KQD82_003068"
     CDS             complement(2739..4052)
                     /locus_tag="KQD82_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG4381523.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4053..6344)
                     /locus_tag="KQD82_003069"
     CDS             complement(4053..6344)
                     /locus_tag="KQD82_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4381524.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6334..7128)
                     /locus_tag="KQD82_003070"
     CDS             complement(6334..7128)
                     /locus_tag="KQD82_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG4381525.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7167..7613)
                     /locus_tag="KQD82_003071"
     CDS             complement(7167..7613)
                     /locus_tag="KQD82_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG4381526.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7685..9091)
                     /locus_tag="KQD82_003072"
     CDS             complement(7685..9091)
                     /locus_tag="KQD82_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381527.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9109..10479)
                     /gene="hydA"
                     /locus_tag="KQD82_003073"
     CDS             complement(9109..10479)
                     /gene="hydA"
                     /locus_tag="KQD82_003073"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG4381528.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10612..>10932)
                     /locus_tag="KQD82_003074"
     CDS             complement(10612..>10932)
                     /locus_tag="KQD82_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4381529.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFVGTEGYYGILKS
                     VYGK"
BASE COUNT         3178 a         2200 c         1140 g         4414 t
ORIGIN      
        1 tagtaaaaat tttattcata ttactattta taatttttat agagcaattt tagtgccaat
       61 atttgcatac atttgtatag tataaaattt ttatgttctc attgataaaa ttttacttat
      121 ttcaagcgtt ctattagatt ttatcatcta aattttaggt tttattttta taaattaatt
      181 gtatttacat aaaaaaaaag acctaaaaac aaatttttaa cggtcttttc tcaatttttt
      241 attttcattt ctaaaattta taataaatag taagaacact ataaattctt ttatataaaa
      301 ttcttagttt gtggatgata aattagtgaa ttaaaatact actttactga ttttagtttg
      361 acaatatcac tccaattttt actagtggct aactctacta aatttaagtc tcttcttatt
      421 tcagctatat cctcttttat agaattagtg tcacctttta tatatgctat gttatgttct
      481 atattatcat gagtagcttt atttatttca gaattatgta ataaacttcc taaaatatta
      541 ttctgttcta tctgtatctc tttcattgat ttatcttcac attctagact atcaatctta
      601 atttcaattc catataattt ttcatttgct atggatacct cgtccttaat tccattcata
      661 tcttcattaa tgttatttat tttattttgc ataaagttta cttctgattt aacttcggaa
      721 acatcttctt tcattacagt tatttcttgt ctcacttctg acatgtcttt tttcattaca
      781 tttatctctt gttttacctc tgatgtatct tctttcatta cagtcatttc ttgtcttact
      841 tcggacatat cttctttcat tatatttatc tcttgtctta cttcggacat atcttctttt
      901 aatacattta tctcctgttt tacttccgac atatcttctt tcattacagt catttcttgt
      961 cttacttccg acatatcctc tttcattgta tttatctctt gtcttacctc ggacatatcc
     1021 tctttcatta tatttatctc ttgtcttacc tcggacatat cttctttcat tacaatcatt
     1081 tcctgtttta cttctgacat atctcctttt aatacattta tttcctgttt tacttccgac
     1141 atatcttctt tcattacagt catttcttgt ttcatttccg acatatcttc ttttaataca
     1201 tttatttcct gttttacttc cgacatgtct tctttcatta cagtcatttc ttgttttact
     1261 tccgacatat cttcttttaa cacatttatt tcttgtttta cttccgacat atcatttttt
     1321 atgctaacta catcttcatt tatagtatat aataactcta aaatcttctc ttgcacttgc
     1381 atcaccatcc ctattaatta ttataacatt ttacacaaat gctatacaat gttaacatat
     1441 atatcagaat tattaaaatt ttaattaaac aaaattgatt cactttatta cttcaaaact
     1501 aattaatatc taaaataact acaaataaac aaaaataagg tcaacaacaa aaaaggctta
     1561 tctctttgag atagcctttc ttatttttat ttaaaacttt gttttttata aaataatcta
     1621 tacattcagc tctgaacatg aaatttttct tccaagtgct tttggtttac aaactgatat
     1681 acaaagtcca caatatctac actcttcttc attcaattcc atcttatcat tcaattttct
     1741 cgcttgatat gcacaaactc tctcgcacat cttacattta gtacattttt cttcatcata
     1801 agtaaactca aagctaagtc tttcattaac atctcccata tgattatgta ctataccaca
     1861 agcattttct atagtattat atcctaatct attaagatta gattttaaat catcatgtat
     1921 tttagatatt acttgtgcac cttttaaaat tactactgaa catataccaa caaagtcagc
     1981 acctgccata atcatttcta aggcatcatc tccacttaca catccaccta agccaattat
     2041 tggtatatta acttgttttt ttatttcata tattctctgt aaagttattg gcttgatagt
     2101 ttctccactt aaatatccta ttccatttcc acctaataaa ggttgccctg tttctatgtc
     2161 tattctaaat gctggtccta cagagtcaca agcagttatt gcatctgctc ctgcttcttc
     2221 taatttttta gcaaaatcgc ctatctcatc tatcatagga ggaagtttaa ctattacagg
     2281 tatatttact ctttcctttg catctttaag cattggtatc aaatctctat agtcataaga
     2341 tactaattca ataaagtcag ctcctgcatt tgcaactttc tctactaatt cgctactttc
     2401 ttcaattgta tgacctattg aagctataca gacagtccca tctcttttca tttgtggtat
     2461 ttcaaaatct atccattgct ctggctcaaa atctgtccat ttttcattat ttaaaagagc
     2521 attagctgta tttctaacca tatgtggtgc tggatttata gctctttctt ttcttatagt
     2581 cttagtcaca actgcccctg ctcctgcatc acttaatatc ttgcatccag ctgcactata
     2641 tgttaatggt cctgaaccta ttataagtgg tgattttaat tctttaccta aaaaattata
     2701 cattatctgc ttcccctttt catatatatt tacattcgtt aaaatttcca aaattcacaa
     2761 gcaacttctt tcatttcttt aacaatagct tttttatcta aatcaactaa ctgtttttct
     2821 tttaaaatat gtctaccatc tatgtataca tttgatataa attctttttt accctgaact
     2881 actatctggt caaatatatt atcaagagtc acaggagttt taaattcatc ttccataact
     2941 attatatcag ccttattacc aactgctatt ttaccagaat tttgaagtcc taatacataa
     3001 gcaccatgct ctgttgccat tctaaatact aaatttgctg gcattactga tgcatcttct
     3061 tcaactgatt tatgcattaa aaatgcacct ctcataactt caaaaaaatc atttatatat
     3121 ccatcagtac ccagtgccac ttttatgcct tttttaatca tttttggtac tggagaaaaa
     3181 cctccaccta cttcacagtt actaagaggc atatgaacca cttttacgcc tttttctttt
     3241 aatatatcta tttcttcatc atttactttt acacactgag atgctaatac agtttcatcc
     3301 aatatatcta aatcattata gtagtcaact gcttttttgc caaagtgttt ttcagcatag
     3361 tttggctcat atatactctc acataaatga aattgcattg gtgcatttaa tttttttgca
     3421 tcttctttag ctttcttaat aaatctatca gaacatgtga agcttgtatg tgtacacatt
     3481 attccattta ttaatttact attttctcta ctccacttta ttaaattaga attttcatct
     3541 aaacatctaa gtccattttc aaagcttatt ctttcacaac tttctaagga taaaatcgct
     3601 ttcatgccta tttcttctag tatttttcct tgctcaatta aagttccttc ttctgtattt
     3661 ggagcttcta gggtatcaca aaaagctacc acaccagatt ctataagttc tattgctgat
     3721 gctttagttg ttgccttcac ttcttttaat ccaattctat tttctataaa tggccaccaa
     3781 tagtcattta aaaaaccttc aaagtcagta aaatgtacat ttgccggtat tcccctagaa
     3841 agaactccat attgatgcat atgaccattg acgaatcctg gcattattat agcatctgat
     3901 ttatctatta tttcttcaat atttttatat tttatttcaa cttcttcatt tggcaatata
     3961 tcttttataa tgtcattttc tactacaaca gcagaatttg tatatatttc atcagcagaa
     4021 gatattaagt atttactttt aatagcaatc atttatttac ctccctgatt taattttttc
     4081 atggccatat aaactttctc agaagttata ggaagttctc ttatacgaac tcctgtggca
     4141 ttgtatattg catttgctat tgctggtgca ggtgtatgaa taactatctc tcctaaagat
     4201 ttagctccat atggacctgt tggctcataa ttatctacaa aatcaacaat tatgtttcct
     4261 atatcttttt ttgttggaac tttgtattgt aatagattag aagtttgaag ctttccatca
     4321 gaaccatgtc tagggtcttc atacattgca agccctatac cttgagttac accaccttca
     4381 acttgtattc tagcaagtgc aggattcata acggtaccac aatctgtaga acaagcataa
     4441 tttattactt taaactcccc tgttgtttta tctaactcta tttcagcaaa tcctgctaag
     4501 aatggtttag cactctcttg tataccataa gattcagatg aatataaaac ttcttgattt
     4561 ccaccaccaa cagattcttt tcctaattca cttaacaaaa caaacttctc tacatcttca
     4621 ctataacaaa ctctgtcttc atatagtatt aaatcatcta ttggtaaatc gagtttcttc
     4681 tgtgctactt ttaatatctt ttgttttaaa ctttcagcag attttattgt agcatttcca
     4741 gttacataag tagtacaaga agcgtatgca ccagtatcat atggacacat atctgtatct
     4801 cctgtataaa cggaaattct atcaatagat gtattcaatg cttttgcagc tatttgtgca
     4861 agtattgtgt cagaaccagt tcctaagtct gaagacccag aaaacaactt ataagttcca
     4921 tcttcatcca tcctcatatt tactattgcc atgtctatgt tggctatacc agaaccatga
     4981 gttcccacag acattccaac agccctcacc ttattgttgc caatatccct aacagggtac
     5041 ttttcatccc atccaataag ttttttacct ctaattatac attcatcaag tgcacaactt
     5101 cttattggaa aattcattat tccgccttca gtttgttttt ttataatatt ctttaacttt
     5161 atttctgttg ggtccatatt aagtttatgc gctaattcat caattgcaca atcaagtgca
     5221 aatgtacctt gtgttgctcc atatcctctt aatgctcctc ctggaactaa attagtatat
     5281 acagttctcc catcaaattt tattgcaggt acattattat atgttggaag tacattatga
     5341 cctgattcag agcaaactga aggtccatta tcaccataag cgcctgtatt gttcaatgct
     5401 ttcatatcta tagcttttat gtttccttct ttatctgccc caatttttat atcaaaaaac
     5461 atctgatgtc tagtattagt cattgcaaaa gtttcttctc tagtataaat tagttttgct
     5521 ggtctttttg tcatccaagt tacaaatgat gcatatatct ctgttacagc tatgtttttt
     5581 ccaccaaaac ctccacctat tcttggtttt attactctca cttttgaaag aggtattccc
     5641 agtgctttag aaacctgtct tctcatatga taagctgatt ggtttgcaga agttactact
     5701 aatctgccat tcgtatctat atacgtataa gctctatgag tttccatcat acaatgggct
     5761 tgtgattgag tcatataaga atgttctact attacatcac tttctttaaa ctctttttct
     5821 acatcccctt tacccatttt ttctctcgat acaatatttt ttgtagcatc caatccaaaa
     5881 tcaaatggac aaaacaaatc atcttcttca tggattaaaa ttttatttcc ttccgattct
     5941 cttgcatcca atataggagt cattacttca tattcaactt ttataagttt catagcttta
     6001 atagcagccc tttcatcttc agcggctatt atagcaactt catctcctac atatctaact
     6061 acgtcttcaa gtattaattg gtcatgaggt gatgcttctg gatatgactc tccaaccatt
     6121 gaatatctat aatttggaac atctttgtaa gtgaatatgt ttactacacc ttttaccttt
     6181 aatgcatttt ttatatctat gttttttatc ttcgcaaaag catgtggact tctaagaagc
     6241 ttaattgtta aaacatcttt atgaaaaatt aaatcttctg tatatattgg ttttccagta
     6301 acaatggctt cactatctat ttttcttacc tctttattta ccatcacact cacctccaat
     6361 actattatac attctcacta ctaaggcttt tgccatatct tttctgtatt ctttacttcc
     6421 tcttatatta cttccaaatg aaagttcttc acttgatact tcaccagctt tttctatatc
     6481 attttctaaa cttaatattt cacttgcttt ataggcaatt tttgctcttc ttggtctagc
     6541 gcctattgca attttataaa tacttccttc ttttaacatt gctccattta atactgcaaa
     6601 atcacctgta cattttctta tagaatcaaa tacacctata gcatcttttt taggtaatat
     6661 aacttctatt aatatatctt tttcaagctc actttctaaa aaatcagcta agttcataac
     6721 ccctttttta tataacttaa cctttgcatc taccactagt aatgaaggta ttaaatctga
     6781 aaatccatac ttagcaaata cgctagctcc tactctagct ccacttctaa attgaactcc
     6841 tactatatta gaaacagcgt ttgaaattac cccattacag taatttttaa taaccttatt
     6901 taattctaaa gttcttaaac ttgtgtctgc tccaattaat atgctatcat cagtttcatt
     6961 tatataatct aaagctaagt cttttaaatc tattgctgag ccaatatttt tactacctag
     7021 ttttaaaaat ccacatcctc caagtattat gttgtttttt cttgatatta gcagttcata
     7081 agcttcatct aaactttttg gaactacata ttcctttata gttatcatac tctctcccca
     7141 tatcaactta attttcactt aaaagtctaa gtgtttaaat atttatgtat tgctcttaat
     7201 tgacctttat atcctgtaca tctacaaagg ttaccattta cataatctat aacttcttct
     7261 tctgttggat tttgaagctc tcttttcatt gccaaaacca tcattataaa tccaggagca
     7321 caaaatccac actggtcagc cccttcgtct gcaagaaaat ctccaaactt ctttgcttca
     7381 tccaagacac cctcaagtgt tgtaatatgc tgaccgtcta ctctagcagt aagataatta
     7441 catgaaagca cggccttgtc atttaccatc actgtacata acccacatga gcctgtatca
     7501 cacccttttt taacacttac atatccattt tctcttaatg tatctgctaa aaaatcatct
     7561 ggtcttatac ttaattcttt ttctttatta tttatggtac atttgataat caaataaatc
     7621 acctcaaaac tcatattttt aagctaaagt tttactatac ttttctatga ataaattaca
     7681 tttattaata aaggctattc catttatgac cttccattct attttttata gttcctctta
     7741 cacaaagtct ctcagctgta tcttgagcat cttttaatgc tttctcttca ttagctgtca
     7801 atatttttga atcttccatt attatatttc catctactat aacactctct atatttttca
     7861 tactagaaga atatactaat gtagataccg gattatgcat aggtatcgct tttggtgaaa
     7921 gcattggatt aaatattagt aagtctgctt ttttacctat ctccaatgaa cctatctcat
     7981 cttccattcc aatagctctt gctccatcta tagttgccaa ctcaagaact ttttctgctg
     8041 acattgctaa tgggtcacac ttattaactt tatgttgaag tgctgttaat ttcataagtt
     8101 caagcatatc ttgagagtta ttactagcgg ctccatctac tcctaaacta actgttattc
     8161 cttttttaag catttctggt actggtgcaa cacctgaaga taagtacata ttacttgctg
     8221 tattatgaga cactttcata tcgtattttt tagtaagttc catatctttt tcagttagat
     8281 acacacaatg taccataagc acatttggtc caagtattcc taatttttct aaaacatcta
     8341 tgtcatattg cccatgcaat tcttttgctg cttctctatc aaaaggagtc tctgatatat
     8401 gtactgtaaa taaagcatca tcatattctt ttactactct ccatagcatt tcaagcattt
     8461 cttgagaatt tgaccatatt gctgcaggag ctactcctat tttgattctt ccattttctg
     8521 tattatggtg tttttcaaac aatcttctta catctttttc aaccgtttct acatcttgca
     8581 ttattccagg atgaactcca aattgagctc ctgtattcat acatcctctt ccaagtattc
     8641 ctcttattcc taattcttta taagcatcta ttattccatc acaaagacct ggcttactat
     8701 gaggatacat gtagtcaacc attgttgtta taccacttct aagtccttca atacatccaa
     8761 gcattgcagc atcataagta tcttttggct ctaaatagtt agctgctgga aatgtcatag
     8821 tttctaacca gtctttaagt accatatcat ctcccaatcc ttttaataaa gtttggaata
     8881 aatgattatg tgtatttata aaacctggga ataaaacttt acctttagca tctataattt
     8941 ttttaacatc agtatattta ctttctatct ctttagaatt tccaatatca gcaatcttgt
     9001 tatcttttac tactaatgcc ccatcaaaaa taacttctct ctctttattc acagttacta
     9061 ttatggcatt cttaataagt atatccatca ttcttatttc cccccgcatt atttagtaat
     9121 ttttctttta atatattgac ctgttggatg ttctttaaca ttacaacttt gtactacatg
     9181 atttcctctc aaaaatacat cttcaacttt acatttaacc ttaaatcctt catatggagt
     9241 ataatctaca ttttgttttt gagttttata actaattgtt tcttctttat tgatatttaa
     9301 aatgactata tctgcatcac ttcctacagc tatctcacct tttttaggat acattccaaa
     9361 tatttttgca gcattcgtag aagtaacttc cacaaactta tttacactta ttctattttc
     9421 taagacccca tatgtatata aaagtgctaa cctatgttca acaccaggtg ctccatttgg
     9481 tatcttactg aaatcatcaa ttcccaagtc tttttgcccc ttaaaattaa atgaacagtg
     9541 gtctgtgcca actgtttgaa tatctccact agacaatcct tcccaaaggt aatttatgtc
     9601 ttcttttttt cttaaaggag gagacattac atacttagca ccctcaaatt tatccttatt
     9661 atataaattg tcttctaata atagatactg tgggcatgtt tctactatca tatctatatt
     9721 tttttctcta gccattttta cagtttcaag tgactcccta caacttaagt ggactacata
     9781 ggattttgaa ttagataatt tagttatatc agcaagtctt gaaacagact ctttctcaac
     9841 tatgttaggt cttgtaagtg gatgatattt tggctctaaa ttaccatttt gtatattttc
     9901 ttctatcaat acatctagta aatccccatt ttcacaatga aatccaacta tacaacctaa
     9961 atcccttgct ttctttaaaa ccctgtatat tgttccatca tctactttca tgccatcata
    10021 tgccatatac atcttaaaag atactatccc atctgaaata agtttttcca tatgattaaa
    10081 tgtatcttta tttaaacatg taattgtcat atgaaaacca taatcacaat aacaatttcc
    10141 acttgctttt ttatgatatg cttctacacc ttgcagtagg ttttccccct cattggcttc
    10201 tgcaaaatct agtattgtag tagttcctcc agctatagct gctcttgttc ctgtttcaaa
    10261 attatctgca gttgtaatag aacctgcatt catatcaaaa tgagtatgtg tgtctattcc
    10321 accaggaatt acaattttac ctgtagcatc aataacctta tcaccattta tttctaagtt
    10381 atttcctatt tcaactatct tttcatcttc tatccttaaa tcacttatat aagtcttatc
    10441 agaggtaatt atagttccac ctcttagtat tgttcccata tatacctcct aacttttaag
    10501 ctttaaagta ttttctaagt attgtaaaac tttatttaaa attgtatttt ctaaaggtgc
    10561 tatattcagc acctctagac tatatttaaa taatttattg tgaactaaat attattttcc
    10621 gtatacagat tttaatattc catagtaacc ttctgttcct acaaataact gttcttgctc
    10681 tatatactca tctattgtgt gagcaagatt ttccttagaa ggaccaaatc ctatagtttt
    10741 tattccagct tcacctgcat agtgacttcc attcgtacag aatgagtatt gagttatttc
    10801 tgaatctatt ccagcttctt ttaatccctt atatgctgct tgaacaaatt catcttcttc
    10861 gtcatataac caacctggga agaatctttc actttctata gtatttcctg tatagcaatc
    10921 agctttttca ac
//
LOCUS       DAENCV010000122        10828 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAENCV010000122 DAENCV010000000
VERSION     DAENCV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.124"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            21..1721
                     /locus_tag="KQD82_003075"
     CDS             21..1721
                     /locus_tag="KQD82_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381530.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1891..2508
                     /locus_tag="KQD82_003076"
     CDS             1891..2508
                     /locus_tag="KQD82_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381531.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2869..4062
                     /locus_tag="KQD82_003077"
     CDS             2869..4062
                     /locus_tag="KQD82_003077"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG4381532.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4080..5372
                     /gene="lysA"
                     /locus_tag="KQD82_003078"
     CDS             4080..5372
                     /gene="lysA"
                     /locus_tag="KQD82_003078"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG4381533.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5589..6248
                     /locus_tag="KQD82_003079"
     CDS             5589..6248
                     /locus_tag="KQD82_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4381534.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6403..7488)
                     /locus_tag="KQD82_003080"
     CDS             complement(6403..7488)
                     /locus_tag="KQD82_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG4381535.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7679..8395
                     /locus_tag="KQD82_003081"
     CDS             7679..8395
                     /locus_tag="KQD82_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG4381536.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8453..9031
                     /locus_tag="KQD82_003082"
     CDS             8453..9031
                     /locus_tag="KQD82_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG4381537.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9425..10297
                     /locus_tag="KQD82_003083"
     CDS             9425..10297
                     /locus_tag="KQD82_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4381538.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4462 a         1129 c         1873 g         3364 t
ORIGIN      
        1 tttttagaga ggagtattaa atgaaacgat atcttaaaac atatggagtt gcattaataa
       61 tagtaataat ttatgcattt attaaattac ctgtactaag attagatttt acatctttat
      121 tttcagtagg aataattttc tttgttgtag caggaatact agatatgatg ttagatagaa
      181 atgaaagagc atctaaaatg gctaaatata atttttcaat agcaatagct ttgcttgtgt
      241 atattgtagt agttcccttt ataacttcta cacctgtact tcatgcaaaa tcatatagag
      301 aactattggg aaaagtaact gaaagtaaat ttactgatga cattagccct gtcagtgtaa
      361 atgacattcg tcttgtagat gaagatatgg caatgaaatt aggtgacaaa aagcttggag
      421 aagttcctgc aatagggagt gtatccaaat taggaaaatt tcatatacaa aatgtagatg
      481 gagagttgta ctgggtagcg cctcttgttc acagagacat aattaaatgg ataacatctc
      541 taagtggtac aagtggttat gtaaaagtat cagctagtaa tcctcaagac gtccaattag
      601 ttcaagaaat agatggaaag ccaataaaga tagtttacca accagaagcc tacttacatc
      661 aggatttaca aagacattta tacatacatg gtattgtaaa tgttggtatg acagatttca
      721 ctcttgaaat aaatgatgaa ggaagacctt attgggttgt aagtttatat gaacataaaa
      781 ttggctatgg aggagcaaat gcaacaggaa tagccactgt tgatgcagaa actggaaaaa
      841 taaatgttta tgatgttaaa aacacaccta aatgggtgga tagaatacaa ccacaaagct
      901 ttgtaacaga ccagattaaa gactggggtg tttatgttaa tggattttta aactctgtta
      961 tatcagaaaa gggagtttta gtaccaacag aaggtacatc attagtttat ggcaatgata
     1021 acaggtctta ttggtataca ggtataacat cttcaggtgg agatgaatct acaattggat
     1081 ttatgttagt tgattctaga acaaaagaag ctagattata taaacaacca ggagctactg
     1141 aaacggctgc aatgaaatca gctgaaggta atgttcaaga aaaaaattat gaagcgacgt
     1201 tccctgtaat gtataatata ttgggtcagc caacatatgt atcatcatta aaagataagg
     1261 ctggtcttgt taagagagtt gcatttgtat ctgttgaaga ttataatgta cttggaatag
     1321 gtgaagataa gaatgaagca ttaagaaact ataaggatgc attagaatct aagggtaatg
     1381 atttaaaact tgataatgat ttaaaagatg aggtattaga aggcacagtg acaagaataa
     1441 gcccagatgt gcgaggtggt aacactaatt attatgtaac tttagattca aataaagata
     1501 taatatttag ggctacttca aaagtatcta gcgaattgcc attgactcag gttggtgata
     1561 aagtcaaaat atcgtattca aaagaagaat ctggtgtaat agaaatgtca gaatttgata
     1621 acctaaatat tgcaaacttt gaagataaaa aagcaaaaga aaaaacagga actgatgaaa
     1681 atacagattc caaagattct gaaaatcaag gtaatgtata atttaaataa aaagattgat
     1741 aatagtattt aacatagtga tagctaattt ttatagaatt taattctata aaaattagct
     1801 attttatttt aattctttgt aaatttaatg tcataaaatt aatataatat tagtacaact
     1861 ttgcaaataa tacttcaagg agaatgtact atgacgaata tggaaaaaga gatattactt
     1921 ttaattagca gggttagatg tgttacagag tctcaattta gaaaaattta tggagaccaa
     1981 aaaagataca ataaaaaaaa ttttaaaaaa actttgagaa aaatgtgtag tgaatataca
     2041 ctaaaaaaat atccttgtga tgtggattat tataattata aagacctctc ttatgtatac
     2101 tatttaaacg gtagtaaact ttttaaggga gatgatttaa taaaggctct agtaggatca
     2161 gaattagttg ttagaatcaa tactgcaggt tgtgatgtaa aaagatttta ccgaaatgtt
     2221 agtgtagatg atgtgaaata cgatttattt attgaatata cagaccaatt tggtaatata
     2281 aagcaagttt tagttgatgt tgacttaaat aatgaaatag acatttctaa atacgaaaat
     2341 ctttctaaaa aaatagataa atctacaatt ccatttttta aagtaccaaa cgttttggta
     2401 gttacaccag ataatctatc cgaattaaat gatttagaga gatatgataa tgagtatagc
     2461 ataaatttta tagatttagg attaaataaa ctatttaatt gtatataagc taactttaga
     2521 aaagaaaact atcgatttgc cacattttgt aaaataattg ttgcaatatt atatagaata
     2581 tgataaaact atattaataa attaattaaa aataatgagc ctagggtaga ggtagctgta
     2641 ttcaatagta ttcaatagga gctggtaagc gttgataatt gatgaaaggg tatatagccg
     2701 aagaaatttt gttgacggac aaattttctg gacttatgca gaatatgcat aaggctgtca
     2761 cttttaaagt ggtgagctac aaggttactt ggtctatgaa atttacacgt ccaaagtata
     2821 cctttgggcg tttttattta tcaaaattta gcttgagggg gagaaagcat gagtataatt
     2881 gttcaaaaat atggtggaag ttcagtagca gatactgaaa aaataaaatc aatagcagaa
     2941 aatataatag aacgaagaaa agaaaatcca caaatggtaa tagttgtgtc tgccatggga
     3001 aaatccactg atgaatatat aactttagca aaggaattga gtaatgaacc tagtaagagg
     3061 gaattagatg ctttaatgtc tactggtgaa atgatttcag catctttatt atcaatagca
     3121 ttaaatgcac ttggatgtaa agctataagt tataatgctt atcagttaaa tataaaaacg
     3181 agtggtcttc atggaaagtc tcaaattgat gatataaacg taaacagaat taaaaatagt
     3241 ttagatgaag gaaatgtcgt gatagtaact ggatttcagg gaatcaacga agatggtgat
     3301 gtaaccactc ttggaagagg tggttcagat acatctgctg tagctcttgc ggttaaatta
     3361 aatggaaaat gtgagatata tacagatgtt gatggaattt attttacaga cccaagaaaa
     3421 tattcaaagg ctagtaaact tgatgagatt gaatatgaag aaatgcttga gttagcgagt
     3481 ttaggagcac aagtaatgca ttcaagaagt atagagcttg cacaaaaata tggtgttgaa
     3541 atctatgtcg gacgtacatg tggtacagaa aaaggaacat atataagagg gggaaaagac
     3601 atgaaattag aggataaagt aataacagga ttagctacta gtgatgatga cagttctata
     3661 acaataaaag attttaaagc tgaaaatata tctagcttat ttgaagatat agcaactata
     3721 ggtataagtg tagatatgat aagccaaact gcacctattt tagataagat aagtgtatca
     3781 tttacagttc caaaagaaga gttgggagaa tgtaaaaaga tagtatctaa atatacagat
     3841 gaagaacatg tagtgattga taataatatc actaaatttt ctttagtagg tcttggtatg
     3901 aaaaatactt ctggagttgc tgctaaagta tttaagatat tcaatgaaaa tggtataatg
     3961 ataaaactta taactacatc agagattcgt ataacttgtg ctataaattc tgatgataag
     4021 caagtagcta tagaaaaaat agcagaagta tttaatatat aaaatttggg gaggctatta
     4081 tgaaacttca tggaactatg actgtaaata atgatgaatt atatattgga ggagtgagtt
     4141 gtttagaact tagtaaaaaa tatcaaactc cactttatgt atttgatgaa gctttagtta
     4201 gacaaaattg tagagaatat atagaatatt ttaaggctag agagggaaaa aataaggttg
     4261 cttatgcagg gaaagctttt ttacctcttt acatgtgtaa tcttataaat gaagaacaaa
     4321 tgtgtttaga tgtagtttca ggaggcgagt tatatacagc ttataaatca aattttccta
     4381 tggaaaaagt tttctttcat ggaaacaata agactaaaga agaaataaac atgggattag
     4441 atttaggtgt aggtaaattt gtggtggata atttttatga attagatttg cttgaagcat
     4501 tatgtgaaga taaaggtaaa actcaagaga tatactttag aataactcct ggtatagaag
     4561 ctcatacaca tgattatata aaaactggac agatagattc taaatttgga tttgctttag
     4621 taaatggaga tttatttaga gcaatagaaa aattaagtga atataaaaat attaaacttg
     4681 tgggtcttca cgctcatata ggttcacaaa tatttgatgt agaaccatac atagatacag
     4741 tggatataat gatgaacttg gtaaaagaaa taaaagaaaa attcaacata caactaacag
     4801 aaattgacct aggtggagga gttggcgttt attatagcga aggagataaa cctaaaacta
     4861 taaaagagtt ttgtgaaact ataatcacta aagcagaaac ttcatgtaaa gagcttggaa
     4921 tagaagtacc tacattggta atagaaccag gaagatcaat agttgcaaat gcaggaagta
     4981 cattgtatac agtaggttct ataaaagata taaaagatgt tagggtttat gtaagtgtag
     5041 atggaggtat gacagataat gtaagacctt cgttgtacca agctagttat gagtgtagta
     5101 ttgtgaataa aattaatcat gaggctatga atcatgtaac aatagctgga aaatgttgtg
     5161 agagtggaga tatattaatt ggagatataa acataatgga tattaaaagt ggagatatac
     5221 ttataacaac atcaactggt gcatatggat attcaatgtc ttcaaattat aataaaatac
     5281 caagagcagc agtagtatca gtatttgatg gaaatgataa gataatttgt aagagacaaa
     5341 gctatgagga cttattagct ttagagatat aaaatggaga agtatataat aaaaattaaa
     5401 tattaaagtt tattatgagc agatttgcta ggatatgtat ctctaaatta atttttggtt
     5461 tattgtatcg ttcgtagcat atctgcttat tttttgtttt actaacaaat ttcttgtaat
     5521 aaatataaaa tattttaaaa actttataat tatgataaaa tttaaagtat atgacaagga
     5581 gggatttaat gagaggaaga gtaattttat taatatcgat attatctata tttcttgtag
     5641 gatgttcatt taataaaaaa gatgaaataa atttagtaga gcaaggaaaa acatatttag
     5701 aaaaacataa ctataaaaaa gctatggaat cattatcctc tgcactggaa gaagatagta
     5761 ctaatgagaa tgcaagagct atgtatatgc aagctatgag aatgtcaaat atgacggagt
     5821 ttgaagaatt aaaaaattat gagggtgcaa tcaaggaact taaacttata gagaaaatca
     5881 aaaatggttc ctctgttatt aaagaagagg caactaaaaa gaaagaagag ttaactaaat
     5941 tagaaaaaga acaaaacgaa gcagaggaac aaagaaagaa aaaggcaaaa gatacggctg
     6001 gagaagatag gtatagagtt gaatcagatg caaggaaatc ttcttatagt ggaagtagta
     6061 aaaataataa atctagttct aaaaacaatg ataaaaaatc agatagtaat aagtctgaca
     6121 ataataacag ttcttctcaa aatcaagaaa agccaaataa tagtaatcca acaccagcac
     6181 caactccaca agatactact ggtggttcag atagtggtgg aaattctcaa aactcaaata
     6241 atcaataagt ataaaatata tgcataaatt taatgaacaa attatatatt ataaaaaata
     6301 tctcttagca attttttctt actaagagat attttttgag aaataattta ataaatagca
     6361 gttaaatttt aaactaaaaa ttaaagtcac agtagagata atctattgta aacgattatc
     6421 tatatattta tcaacataag tttttacaac agcagctatt ggtactgaaa gaaaaactcc
     6481 tatgaaacca aatagagatt gtcccaccaa aatagcagat attacccata tagctcctat
     6541 accaacttga ttacccataa cttttggttc tataaaattt ccatctaatt gttgcaatat
     6601 aaatagaaat gcaatgaccc atatagtttt ttgtggacta tataatatag tcatggctat
     6661 tggagggatt gcaccaataa aaggaccaaa atagggaatt atattaccta agaatattat
     6721 tgaagctaaa aataaagcat tttctattct gataacaaat tgaaatccta aaaatgcaat
     6781 tataccaata ataagagagt ctaatattct accaagtaaa taatcataga aaatatcatg
     6841 agtagtccta aaaaattcta taatgttatc tgccttgttt ctagcagtag aagaatatag
     6901 aaatctctta aatcccaatg caattttttc tttgtcatat ataatgtata tggatagtat
     6961 aaatcccatt gtcatattaa atagcataga agatatacta agtgtataga ttaagaagtt
     7021 tttaggaatt ttaccaacaa aattacttgc ttcttttaat aaattatcca aactatgttg
     7081 tatatgagga attaaagttt ttagaaaatc tatgtttgac atattttttt ctataaaact
     7141 atctatataa tcagtataca taggcatttc atttattaat ctatttaatg tattagctat
     7201 tgatggaatt aataacctaa tggcaaaggc taaaataaaa cctataaata tataagataa
     7261 aaaaatggct aaaagtctat gtgctttgaa ttttacctca aaaagcatca caagtggatt
     7321 taaaagtaaa gcaagtaaaa tagctaataa aaatgggcta aaaaacttta agaatccttc
     7381 aatactagat ataaagtctg atggagtatt aataaattta aaaaataaaa ttccaataaa
     7441 cacgactatt aaataatatt ttatattttc ctttgaaata ataatcactt ccttaaaata
     7501 gtgtttagtc agctattaca tattaatagt atgtgaaaag aaattacaat tatttaggaa
     7561 aattaaataa tttttttgct tgtttttaat taaggtctag tatagtataa taatgtagca
     7621 aaattaacat tgataaaaat attaaacaca cataatttaa gataaggaag gattaagtat
     7681 gagaattaat aagtatatag cttcttgtgg aatagcatca agaagaaaag cagaagaaat
     7741 aattcttgga ggaagaataa aggtaaatgg aagtgtagta aaagagttat cttttaatat
     7801 tgatgaagaa aaagacatag tagaatttga taataaaaaa gtaaaaccaa gtgaaaatta
     7861 tatatatata gtattaaata aaccagaggg atatataact acagtaaaag accaattcaa
     7921 tagacccagt gtaatagaca tacttaaaga tgtaagggaa agagtatatc caatagggag
     7981 attagattat gaaactagtg gtttattaat acttactaat gatggcgatt taacatataa
     8041 gcttacccat ccaaaacatg aaattgataa aacgtatgtg gcaagtgtga agggaattat
     8101 aagtgatgat gagataagac aattcgaaac aggactaaaa atagaagatt acacaacggc
     8161 acctgccaaa ataagggtaa ctaaagaaaa taaagaaaaa aattattcag tgtgtgaaat
     8221 aactatacat gagggtagaa atagacaagt aagaaaaatg tgcaaagcta taaatcatcc
     8281 agttttaaat ttaagaagga tttctgtggg gaaaatagta ttaaaagaca ccaaagttgg
     8341 agaatataga tacctaacag aagatgaaat aaaatattta aaatcaataa aataagataa
     8401 aaataaattg agaaaaaatg aaataaagtt aatagtaggg agaaatagta ttatggcata
     8461 tgcaaagtat ttaactaaaa ttacagatat aaataaattt tatttagaaa atattataaa
     8521 tgaaggtgac atagtaattg atgctactat gggaaatggg tatgatacaa ggtatctagc
     8581 tgagaaggtt ggagaaaagg gctttgtata tgcttttgat atacaagaag aagcaataaa
     8641 gtctacaaga aagaaactag agaaagaagg atatacagat agagtaaaat tgatatttga
     8701 tgggcatgaa aaaatgaatc aatatataaa agaagaagtt tcatgtgttt tatttaattt
     8761 gggttatctt ccaagagcga aacattcaat tataaccaaa ccagatacaa ctttaaaggc
     8821 tataaagtta agtttagaaa tgctaaaaga aaatggagta ataagcattg ccatatatac
     8881 agggcatgat ggaggtcaag aagaaaaaga ttgcatatat aaccttgtca ataatttaaa
     8941 tcaagatgaa tttaatgttt tagaatctaa gtttttaaat caagtcaata acccaccaca
     9001 attgatttta attgaaaaga aaaaagcata ataaatacta aaaaacttga caagattgta
     9061 ttaagagtta atatatatag tgtgacctat taaaaatact ttaaataata agtatacaac
     9121 agcttattct caaaacaaaa tatacatcta aatatgcaaa ataagataga taacttttaa
     9181 atgagtaaaa attgaaaaat ttaaaagaaa tattggataa atattaaaaa atgcaaggaa
     9241 agatggtata ataaatttaa tcgaaaatac tatgtgaagt ataaggtaca gaaaagttag
     9301 aattttatga gtttaagaag ctatgataaa aactaagatg aaattgagat aaaaaataaa
     9361 atttttagag aaaaagacag tgttaataaa acaaaatagt ttatataact aaaaggagaa
     9421 aaaaatggaa gaaacaaatg aaatgaagga cagtaaacat ttgatatcaa atatacaatc
     9481 tcaatatacg agattgagta agggtcaaaa acttatagca caatacattt taaataatta
     9541 tgataaagtt gcatttatga ctgcatgtaa attgggtgaa actgtaggag taagtgagtc
     9601 tacagttgta agatttgcaa atgcattagg ttatagtgga tatcctaaat tacaagctgc
     9661 acttcaagaa ctcataaaaa ataaactaac tacagtacaa agagttgaaa tggcacatga
     9721 ttattccgat gattttgcaa tactaaacaa agttttaaaa agtgatatag ataatataag
     9781 gtctacacta gaagaaattg acgagagagc gtttaaagaa gcttctaaca aacttttaag
     9841 agcgagaaaa atatatatac ttggaatgag gagttctttt gtagtagctc aatatttagg
     9901 tttttactta gatataatac ttgacaatgt tcatataatt agaatggata tgggagatgc
     9961 ttttgagcag attgtaagaa taaatgaaga agatgtaata gttgctatta gtttccctcg
    10021 ttattctaag aaatcttatc aaattgtcaa ttatgctaag gaaaaaggag cccatgtaat
    10081 atcattaaca gatagcttat ttgcgccagt agcatcactt gcagataaca ctcttctagt
    10141 taagagtaat atggcttcat ttgtagattc tctagttcca gctcttagta tttcaaatgc
    10201 ccttgctata tctgttggga tgaaggaaaa agaagatatt aaacagcatt ttgatgattt
    10261 agagcaaatt tggaaaagat attctgtgta tgagtaaagt ttgtatatta ataaggctat
    10321 gaaattgaga atcatagcct tattagtaaa tactcgatta tcacttaaaa aataaatttt
    10381 cgtttaaata atacaaataa atatttcgag actcaaaaca taaagatgta ttctgaatac
    10441 atctttacta cgtcaactaa tttattaaag aaagcatttc cattcatgga gagtgctttt
    10501 tttatttctc taaacaatat catttcctta attaacaatt ataaaattaa ttttatttgt
    10561 atttaagtat ataatatctt attaaaatta tattaaaata tgtatttcta tcttttttgt
    10621 tcactataat atgtgtcttt tagtatttta ttttaacttt gacaaagaag ttgaaaaatt
    10681 taaaaatcat agaattagta gttaggtatg aaatcttgat tttttaagta gccatttttg
    10741 gggaaaggga aactttttta gaaatataat actttataaa aataaaataa agtttttata
    10801 aatattaaga catagaatat aaagcttt
//
LOCUS       DAENCV010000123        10785 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAENCV010000123 DAENCV010000000
VERSION     DAENCV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.125"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(119..1477)
                     /locus_tag="KQD82_003084"
     CDS             complement(119..1477)
                     /locus_tag="KQD82_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381539.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRVL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1925..2377
                     /locus_tag="KQD82_003085"
     CDS             1925..2377
                     /locus_tag="KQD82_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG4381540.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(2534..3151)
                     /locus_tag="KQD82_003086"
     CDS             complement(2534..3151)
                     /locus_tag="KQD82_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG4381541.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGVKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3310..4008)
                     /gene="yunB"
                     /locus_tag="KQD82_003087"
     CDS             complement(3310..4008)
                     /gene="yunB"
                     /locus_tag="KQD82_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG4381542.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            4222..6915
                     /locus_tag="KQD82_003088"
     CDS             4222..6915
                     /locus_tag="KQD82_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG4381543.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQTT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLILGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     GSTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            complement(7190..8125)
                     /locus_tag="KQD82_003089"
     CDS             complement(7190..8125)
                     /locus_tag="KQD82_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG4381544.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(8169..9341)
                     /locus_tag="KQD82_003090"
     CDS             complement(8169..9341)
                     /locus_tag="KQD82_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG4381545.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            complement(9328..9792)
                     /locus_tag="KQD82_003091"
     CDS             complement(9328..9792)
                     /locus_tag="KQD82_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381546.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(9807..10649)
                     /locus_tag="KQD82_003092"
     CDS             complement(9807..10649)
                     /locus_tag="KQD82_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG4381547.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         3655 a         1856 c         1393 g         3881 t
ORIGIN      
        1 tatagccttt atttatgtaa tttaatcacc ttatatatca tacttataat tatgataata
       61 actacataaa aaatcacttc taaatagagt tatccatcta aaagtgattt catatattct
      121 atgcaacttt gtgttttagc cactttgttc ctcttaatct taaacaatat aaagtccctc
      181 taacaagcca atcagtatac atacctatcc aaattcctaa cactccaaat tctaatacaa
      241 cccccaatac gtaacctgta aaaattctaa aaatccacat acctattact gcagtcatca
      301 ttgtatatct agtatcaccg gctcccttta gccctgctga taatacaaag gatataggcc
      361 atattaccat agctaaacaa tcacttcgaa ttaattgtgt tgagatgtca attacactag
      421 cattttgagt atacatactt gaaatccaag gtgcaattgg tataaatata gctcctacta
      481 taaataaaca aattgttgca aatttagtta aatataataa agtactctta gctcctttaa
      541 tatctccacg tcctatatac tgtcccacca aggttgtagc agacaaagct aaagcattcc
      601 caattacatt tattatggat gcaattgaca ttcctattgc attggatgca attgctgatg
      661 ttcccatagt aactataaat acttgaacaa tcaatttacc tgcattaaaa agtagttgtt
      721 caactccagc tggtattcct atattaaata tagacttttg agtttcaaca tcaatcttaa
      781 atggaaatat tttacccata gatataaatt cgtttccttt aaataaaact cttataatta
      841 ctatacatcc aattattcta gctatagcaa tagatatagc tgcacctgtt atgccatagg
      901 atggtgtttg caaatttatt cctagaggat ttaaatcaat accatatata agtatatatc
      961 ctaatgttac atttactata ttcataaaca tagtcacata cataggtttc tttacatctc
     1021 cacaccctct taaaatacca tttgctattt gttgtgtcgc aataaatgga tacgtaaata
     1081 atgtaaactc tatgtataat tttgcatttg attttaccaa ttcttctgcg gaaccataaa
     1141 ataaattaat tacaccagtt ctaaaaaccc acatcaatat tgttattgta aaggataata
     1201 ttactccaga cactagagcc tgcttaatag tttcattcac cttttcacca tctttttttc
     1261 ctatttgttg agctacaaca acagtagctc caacagatag tgcagcaaat acagatataa
     1321 atatattgtt tattgaatca accattccaa ctgcagatac agcttcttcc ccaatatgac
     1381 ctgccatcat tgtattaaat actcctaaaa gcatgacaaa cgtctgctct atcattatag
     1441 gaattgctaa ctttataaca tttttcctta taaacatccc tattcctctt ttccctcttt
     1501 atataaattt aatttttaat tagtttatcg aactgtaaat tgacaatgca aagtttagaa
     1561 ttgtattgtt cgcattataa tatactgctt tcaaataata taaatcttgt ttaatatcat
     1621 ttgaacacag tatatcttta taattatctt atgtttatta ttttttatgg ataccacatt
     1681 taaaatattt attaatttaa tataaatatt ttccgttatt attaaaagta gctatatttc
     1741 gttaatattg tatcactatc gtgtagcaat aacaataagt acattttata tattttttat
     1801 aatattattt aatcatccaa acttttacca ctttttcaat atataaaata ttacacttta
     1861 gatgtaacaa aatcgcatcc atttaaatat tgtattttat agactaattt taggatgtga
     1921 tactttggtt aatttaaatg caaataatct aaatgtaaat atggtttatc ctatttcttc
     1981 cacagcaccc atacgtttca gaagatatac tgtttcatat ttagaaaaca tggaaaatat
     2041 ttatttaact atagcaacat actttgatac acttggttta ggcacaatga atatcgacaa
     2101 agtttatggt caatgggctt ggtttactga taatatatat gatttaaatc tatttgtttt
     2161 tgtaggaaat tatccttatc aaattgctaa aaacagatat gatacatttg tagatatttt
     2221 gccagtagct attacttcta ttgtcaatgg agatagagta tttttaattt caaatccttg
     2281 tttgttaaat tcaaaaatta cagttagatt tatatctagt catcctactc ttaatagaac
     2341 agttgattat ggcaatatta gagattttat tttttaaaac tgcaacatga tgtgctatta
     2401 aaaaataccc taaatttaat gatattatta aataactaga atatttttaa ttggcacatc
     2461 atgcctagct attataaatt cataatatta ttttacttaa gataagtact gtattttatc
     2521 tacttgtata tttttattta cctagtttct cagttctata tcctatttct ttattagcta
     2581 gcttataacc ttcttgtatc atttcttctc tcgttaatgc actccctaat aaattgtcaa
     2641 tactcagttg ctttctatca ctaaaatact tatccaataa tgattcctct atatcttcct
     2701 gatatgccaa atttccttca tgcattatat attttacatc aaaaccttct ttatttaata
     2761 cttttcccac caagatactt ctatgacatc ttataggttc ttgcatagct cctaataaaa
     2821 ctatcctata acccatttga caaccctttt ttaatctttc tattccgttt aaaaaaatat
     2881 cttcttttgc tactttctca aaatctgcat aaccttcttg cgtatataca tctttattag
     2941 ttcttttaac tccaaattct tttcccatgt atatatataa aaatccggaa tttctaagag
     3001 attcgttaaa tgcctcttta ttgtattgaa tattatattt agaatatgga gtttctctaa
     3061 tgtcaactac acaatttatg tcatattttt ttatcatatt aataagctta tcatatggat
     3121 aatttgaatg tcctattgcg aatatttcca tataaacacc ctcctattct ttgaaattag
     3181 tatctatata tgtagatacc cgatttactt aaaattaaat aaagtattac tttaaatacc
     3241 caatatcttt ttttcttgat attgtatatt tttttacttt atatccttaa aaaaatatac
     3301 aactttatat tacttgttgg tattctttcc agtgttaggt aaaacatttc ctagattttt
     3361 ttctggaata tttacataac tttctggaac atcacctact attatagttt cacaaatagg
     3421 tatttgagct ttaatttgtt ttgttgaagt tataagtggt attactactt taacttgtgt
     3481 ttgagcttct aaatatattc tatgtctagt ttgatttata cctgaagact caaattccgt
     3541 tttaaagttt actgatactg ttcctatagg ttcaatagat acttcaagtt taggaccgta
     3601 tttagctaat ataggacttc ctaatgctgt tcctataggt atgtaagaac ttgctgtctt
     3661 atcctttttt aattcatctt gtatttctaa agctattgca gatgcaattt cattcatcat
     3721 tatagtgttt gcttgtatca tagttatttt accattatta tcaagttgta tatccattaa
     3781 atcttcataa tttatttttc cttctaccat ttcagcaaca gctttattta tcgacctatt
     3841 tgctagttca agagctttag tctctgctaa aactgttatt gttggtctta aagttttatc
     3901 tatatatata aaactgccta taaatacaga caagaaaaga attactaaaa aaatagctat
     3961 gatttttcta aattcatttt ttgtttttct atctactatt ctattcaaaa ttatcccctc
     4021 ctatttattt atatgtagct ttggcataat ttttataata tttgtcaaaa ataaataaaa
     4081 taaaaatata gactttttaa tatattacgt ttatacatat acaatgtaca gaatattata
     4141 gcaaattcat tttttctatt aattacaaaa ctactcctat ttgtgttata atattgcatt
     4201 aagtaaggag gttaaaatct tatgaataac gataatgata aaaatggaaa taaaatcagg
     4261 agaaaaaaag ttagctcatc tggtaataca aataaaccaa ttaatagaac ttcagctaac
     4321 aagactcgtt caacaaaaaa caagaaaaaa tctaaaaaaa gcgataaatt taaaaaacta
     4381 agagtatttg ggatagtatt tttagtttta ctagttgttg gtacagcagg tactgctggt
     4441 ctagtatttg cgtcactaag agatgtaact ccagttacag aagctgtttt agataaacag
     4501 acgaaccaaa caactactat taaatacgct aatggaaaaa cattatctac agctccaagt
     4561 gtaaataaaa agacacccgt tccattagat aagatttccc cttatcttca acatgctgta
     4621 attgcaatag aagatgagcg tttttatgag cataagggtg tcgatattaa aggtttattt
     4681 aggtcagtat taaagaccct tacaggtact aagcaaggtg gtagtactat tcctatgcag
     4741 gtatccaaaa tgctgttaac aactgaacag caaactatac ctcgtaaaat aaaggatatt
     4801 tactatgcac atgagatgag taaaacagta agtaaagata aaatactaga aacttattta
     4861 aataacttct tcgttggtag aggtctagca ggtgctgagg ctggtgcacg tggttatttt
     4921 gataagtctg cagctgacct tactctagca gagtcagcac ttcttgctgg ttcaactaaa
     4981 aatccatcaa gattttcagc ctataaaact tctaagttag agggaaatga aactaaggag
     5041 gatttagaga ataaattatt attttttgtt aacacaactg atgatgattt agatgaccct
     5101 actcaagtgg actttgatat gattgaaaaa ataaaatctt gggagcttat atctaatgat
     5161 acttatagac aattaaaagc aggtacatta gttgttagaa aggctgtaag taatcctgaa
     5221 gctaaaaaaa gacaagaaat tgttttaaag aaaatgttag aattgaaata tataaaacaa
     5281 agtgaatatg atgaagcaat caaagctaaa atagaaataa aattgcctca atcatcagat
     5341 aaagtttctt catctgtaga agaccttatc gaaagtgaag ttataaatgc tttaatggaa
     5401 caaggacata caaatgatga agctcaaaac ttattctaca atggtggtct aattgtaaac
     5461 acaactatag accctaaaat gcaagatgct ttagaagaag aatttgatag aaatagcaat
     5521 ttcccaggac atatggttgg tcctgatgga gttagccaac cgcaagctgc aatggtaatc
     5581 ttagattata aaaatggtga aataagagca ctagctggag gaagaaatat aagtggtaga
     5641 aaaactttaa atcgtgctac taaccctcat cagccaggtt cttctataaa acctttagca
     5701 atatatacgc cagctattga tactctaaaa ataacacaaa caactgctct aagtgattct
     5761 cgaggaggat ataagtttga agaaaataat aaatggaatc caagaactac tactgctggt
     5821 catggttcta tgagtttacg taaagcactt gctaaatctt caaacacgat agctgtcaaa
     5881 actgctgaaa tgcttggaga ctcttatgat gaatgtgtag atataatgat ggattactta
     5941 aagaactttg gtataacaac tttaaagaat aatcactcag gttcatctga agcaagtgat
     6001 agaaaattcc catcattgat actaggtggt atggcgaatg gtattactcc acttcaaatg
     6061 gctgctgctt atggtacatt ggcaaatcac ggtatttatg tagaaccttc tatttttact
     6121 actataacta cctttgatgg tcaattatta gttaaaaatg ctcctgagga acataaagtt
     6181 gtagaacctg aagtagctta tgtcgttaca gatatgttag aaagtgtaat tactgaaggt
     6241 actggtggtg ttgctgcact tccaaaggga atgcctgtag caggtaaaac aggtactaca
     6301 aatagtgcat atgatgcttg gtttgtcggt tacactcctt attatgttgg tgcaacttat
     6361 attggagatg atgctggtag aaaagatgat tctggtaaca ctataaaacg tagagaagta
     6421 ccacatggaa gtactagcac tgcaaaatta tgggaaaaaa ttatggaaaa aatacatgct
     6481 aatttaacgg ttactgaatt tgaagttcct aaaaatgtat attttacaaa aattaattta
     6541 gaggatggag gaaagcaatc ttctggctct aaagcagcat ttattgaagg tactgctcct
     6601 actaaagtaa gttctcaacc atcatctgaa gatacaaaaa aaccagacaa taaccaacca
     6661 gaagaaaata ataataacaa taataacaca ggtgataatg gtggcggttc tacacctcct
     6721 gataatggcg gaaacaatgg tggtggctct actactcctc ctgataatgg cggaaataat
     6781 ggtggtggcg gttctacacc tcctgataat ggcggaaaca atggtggtgg ctctactact
     6841 cctcctgatg gtggtggaaa taatggtggt ggctctacta ctcctcctga tggtggtgga
     6901 actccagcaa cttaataaaa aatagaattt aaataaaaat ataaggagtt gtctcaaggt
     6961 gattaaaaaa atcactttga aacgctcctt tttttataat tacaatatat tatttgtatt
     7021 cataaaaaag agaatcccaa taaccagaat cctccttttt tatataaaca aataaatatt
     7081 ttatttttaa agtttttaac taagtgcttt attcttactt ttattcctaa ttgtttatat
     7141 attcttaaat agttatatca caaattatat aaacttaata tttttctaat tactgtatac
     7201 caaatgtttg aaatctattt aatttgccat atctcttaat aagcatttcc cactcttctt
     7261 tatcatagaa atcacactta tttctaccaa atagcttggc aacttcaatt gcaaatctcc
     7321 cagctgaatc taaatcacta aaattagttg cacctgtagc acatccagca acaggcatct
     7381 ctgttgtaat tgctactcct actattggtg catctgtagc tactgctggt tgtaaaatac
     7441 tatttaagtg atacagatta tttccatatg gtgttatatc ttgaatagac aaagggaata
     7501 caaatggaga tttccctgtt acagtctgca taatatctaa aaggttatca ctagttttta
     7561 atatgtagcc ttctttaaca gttggcgata ttgcaaatcc tctagtattg attactctat
     7621 ttcctttagt agtatctata gaaagtattg catcacaact actatcaact tcattttcat
     7681 tcatagtcat catatcaatt ggagaatcca taaaaggagt aggatagtgt tctttagttg
     7741 gagcatctgg gcaaatatga gttgaaatta caacatcacc atctaatata tctccttttc
     7801 gttgcatatc caaaagttta gcagccactg caattgctac caatgctcca tcaccatctg
     7861 aaacaaaacc tataacttct ggtcttgctc caatccctcc tagtctacct aaaatgccaa
     7921 gagtaggagc atctccacct tttaattttc catttttccc aggaattgta agctttacta
     7981 aatctgttga gcctttactt gaagacaatt cttttacagt tacatctact tcaccataac
     8041 cttgtaagta agtttttact tcttccccat tagcatttgc tttatctaaa atgtcataaa
     8101 cctctattac ttgttttaat atcatgtcta atctgatttc ctttcttaat atattttaaa
     8161 attatatatt atttactata caaaatatct actccacaca ttgctagcac cttaattgct
     8221 tttataagtg ctactttgcc ttcttcacca ccagctgcaa cagcaaattc atctgtatga
     8281 gttgcagtat tttcttttaa ttttatatat gcttgtattg caggtatctc atgagttaaa
     8341 tttcctgtat cagttgaacc tataccttga cttaaatctc tttcaacata gtcttcaccc
     8401 aaaacttcaa aattttgtct aactatattc actaattcag aatcattctt aatctctttg
     8461 tatatttcat ctaattgaac tatttctaca ttagttccag tagaaattgc agctccttta
     8521 gcacaatttt taagcatttc acaaatctca tttaaatatt ctatgcttaa agctcttaca
     8581 ttaaatatag ctgtagattt ttcaggaatt atattatgta tgctacctcc atcagttata
     8641 attccatgta ctcttgcata atctttaaga tgtaatctca tgctatttac actgttaaat
     8701 agtgctatta ctccacttaa ggcacttttc ccttcccaag gaaaagcagc agcatgtgat
     8761 gccttaccag taaaatcaaa ttttaaatta acttgagcaa atgatatatc atcaggcatt
     8821 gaagcatcac ttggatgtaa aattaatgca gcatctacat tattaaatac accttcttta
     8881 atcatagtta tttttccacc tactctttct tctgcaggtg ttccaaaaac ctttacagtc
     8941 ccttctatat tatgtttttt catgatttct tttaatatga tacctgcacc aacagaactt
     9001 gtccctatga tattgtgccc acatgcatgt cccattcctg gaagtgcatc atattcagct
     9061 aaaaaagcaa ctgttttccc attagttcca tttgaataca cagcttcaaa agatgtttct
     9121 aatcctccta cacctttagt tacattaaat tcattttctt ctaacaaatc acataatgct
     9181 ttttgtgact tatactcctc aaatgctaat tctggatttg aaaataaata gtcagaaact
     9241 ttttcaaatg atgtcttttt ctcttcagat aataacttta tttcctcttt aatttttgct
     9301 tttaattcat tcatatttac tacctcctta aagcattcca tttaatctta aaactgtttg
     9361 agatataaaa gcagaaccaa agtaagtaga tgtcataact ataaatgata taactactaa
     9421 cttccaactc aaagtcttta atatatctaa ttggttacct acagagattc ctgcaaaagc
     9481 taataatggt gttactgtag acataaaatt gattttccct acatttgttg ctatgatatc
     9541 ccccactgga gatattggaa gcgataaaac aaatgcaact aatgtagccc atgcaaaacc
     9601 tggtaaatta ggttttttaa caaaattctt tattataata gcaattatca ccaacaaaaa
     9661 tccagataca cttccaatca acaaatcttg agcctgagtc ttaagtgcta tcatttgtcc
     9721 aaagcaaata ataatatata taaacaatac tatttttatc tgctccataa tagtcttaaa
     9781 agttttttcc atatatatat cctcctttac aatttattac ttgttcttct tgtttttctt
     9841 cctctaaatc tttcaataac tggagctact ttgctataat agtagttggc tactggtaaa
     9901 gataagaata attctatata tattccagta acagctgtaa tcaatccact agtagctgaa
     9961 tatgctaaaa tctgctctga catttgtgga actgtattca ttaatgatgc tgttgcagct
    10021 cccatcattg aaccactacc cattcctgat gccattgcta aagcatatgg atgaaatcct
    10081 aaaagtacac ccactggtgc taaaaatgaa aataataatg ttccaaatat actaccactt
    10141 atgtaagttc ctagtgttcc tataccttct ggtgagttta atccatactt atctccaata
    10201 attcctagtg ctgtgtcacg acataatgaa aaagtaccac ctaatgcact tcttccaaga
    10261 cctaatgcta ctccaacagg taatgctatc aacattgtac ccatatgacc aaattcttgt
    10321 aataaaagtg ctggtcctgc ttgaagtaat ataccaactt taggccctgc attaattcca
    10381 aacattatcc ctaatggata tagtgtcaaa gataataaat atggagatac atttatttca
    10441 ttatccccta taaacttttt taaggctggt ataactctcc ctaataaatg aggagaaaca
    10501 gccattccta tgaatatagc ccatagtata ctaagtaaag ttacattaat tcccataact
    10561 ttaaatgaaa taggtccaat taattctgcc aatattgtga ttataaaggc tgctattatg
    10621 gcatttctaa tccctgcttt ttctttcatt tctatacctc cttgtaattt ttataatttt
    10681 cttataatat aattgttagt gattttatta tataacctat cattgataat gtataatata
    10741 tatttattca tcatttttat atacttttag ttatgaatca aaaaa
//
LOCUS       DAENCV010000124        10726 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAENCV010000124 DAENCV010000000
VERSION     DAENCV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.126"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(111..932)
                     /locus_tag="KQD82_003093"
     CDS             complement(111..932)
                     /locus_tag="KQD82_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4381548.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(1450..1926)
                     /locus_tag="KQD82_003094"
     CDS             complement(1450..1926)
                     /locus_tag="KQD82_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG4381549.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(1960..2538)
                     /locus_tag="KQD82_003095"
     CDS             complement(1960..2538)
                     /locus_tag="KQD82_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG4381550.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(2642..5242)
                     /locus_tag="KQD82_003096"
     CDS             complement(2642..5242)
                     /locus_tag="KQD82_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG4381551.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTNKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(5421..6104)
                     /locus_tag="KQD82_003097"
     CDS             complement(5421..6104)
                     /locus_tag="KQD82_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG4381552.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(6324..6914)
                     /gene="zapA"
                     /locus_tag="KQD82_003098"
     CDS             complement(6324..6914)
                     /gene="zapA"
                     /locus_tag="KQD82_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG4381553.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(6939..9332)
                     /locus_tag="KQD82_003099"
     CDS             complement(6939..9332)
                     /locus_tag="KQD82_003099"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG4381554.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(9349..10368)
                     /gene="pheS"
                     /locus_tag="KQD82_003100"
     CDS             complement(9349..10368)
                     /gene="pheS"
                     /locus_tag="KQD82_003100"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG4381555.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(10418..10652)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3236 a         2119 c         1103 g         4268 t
ORIGIN      
        1 tacaatatat ttaaattata actcatttat atgtctatta caacaaattt accctgctaa
       61 atacctactc tatatcccat aattttacaa gttgtagtga agtttaagtt ctatctcacc
      121 tcaaatttag cagtgtttct atttacagtt cttttatatt tcacatctgg ataacccaat
      181 ataaatgaag tgataacagc ttcgttctcg ctcattccta acagttcttt tatctttggc
      241 tctacatttg atgccataac aaaaaatcca ttataacaaa ttcctaatcc ttgagaatag
      301 gccataagtt ccatattaga tgcagccagt cctccatcta cataagcact tccacctaga
      361 gacatatctc ctacaactac cataagtagt ggggcatcat aaaataaaga atcatttccg
      421 ttctctttat atcttttgta cattttctta aatgtatttt tatagataga acgtacaggg
      481 tcattgtcat cagtatctaa tgccaaatta taaagacctt gtattgccag ttctttcact
      541 tcttttagct tttcttttac taatatgtat cttataggct gtctattacc acctgttggt
      601 gtgtatctac cagcttccac tatgtttttt attttttctt cttctacctc tatatcttta
      661 tattgtctta tacttcttct aaatttaata aaattcaata acttttcggg ttctaattca
      721 aaactttcac gattgtattc tattacttca tttttatcaa atccttgcat ttctattgca
      781 tctgttgggc aaattgccat acagtgacca cagttaatac agagaatatt tttaaccttt
      841 gctttatcat ccttaacttt tattgcattt acaagacagt ctgattcaca cataccacag
      901 cctacacatt tttctaaatt tatgtctatc attccaaatt attcctcctt ttaaaaaatt
      961 tactttagta taaaacaaat agttgtctaa gtcaattttt atttttttaa caatatacta
     1021 gccttaacta cactttaatg gagaaaaata tacataaatt ggaacttaaa ttagaactta
     1081 ttcaaagtaa aagataaaag aagtagatgt ctaaaagtga actttttagt taattagata
     1141 ttctttattg tatgaaatca agtggatatt tatcatttca acaataaaaa aggcttacct
     1201 ctattttgga tatgtccctg tcaagtagac agactaaaaa aaggctatct ttatgcactg
     1261 agatagcccc ttttcccaac tactattgtt tatcttaata ttagtcaata aatttaattt
     1321 gctataacct gtaagttaca ttttaatttg atgtaacttt aaattacatt tttaagtaat
     1381 atctttaaat tatattctta aaatatatca ttaaactaca ttttaattaa tctaaaaact
     1441 atatcttaat taatctaaga aacttacatt ataatctttt agtttcccat tatcatatac
     1501 aatctttgca ctaaagatat ttccagtctt agcccaattt aagaacagcc tgtcaccttc
     1561 ccacatattt aaatcgaata ttttatcatt atcaacccaa attaaatttc cttctttaca
     1621 atcatttaaa tctttttcag ataattcacc ttcaaactca tctgagaaat acaaaaacat
     1681 ataccaatct tcttcatctt taaattttgg aaaagttatc aaccctttat atgacaatga
     1741 tttaagagta agaccagtct cttcttttac ttctctaatt acacattctt ctggggtctc
     1801 tccttgttca aactttcctc caactcctac atacttacct tcatgtatat catctttctt
     1861 tttatttctg tacaacatca aagtcttgtc atctttttct atataacaaa tagttgtaag
     1921 tttcatgtat ctcgccttgc tttctcttta tattctcaac taattatttt cagcactttc
     1981 aagcacatca cttatattct tcattgcatt aagagaaagc tctgcaaatt catcaaatgg
     2041 aatccctata tcttctatac ttctcattgc atctctattt acatttgaag caaatctcgt
     2101 ttctttcatt cttttaataa cagacttagt ctttacactg gtaagttttt tatctggata
     2161 aactaaagct atagcagtta taaacccagt tatagggtct gctgcatata ttgctttttc
     2221 cattaggcta gttcttgtaa ctcccattcc atcatgatga ccaagtattg ctctatacat
     2281 ttcttcgtca ccgaagtttt cttttttaag cagttctact gttgttgtag catgtccttc
     2341 acatatttct cctggctttc ttccaactat atctgaatcc aaatcatgta aaagacctgc
     2401 cactgcccac tcttcttctt tttctggctc tagtcttttg gctaattctc tcataacagc
     2461 ttcaacagca taacaatgtt ttctcattct atcagtttct aaatacttaa ataaaatttc
     2521 taaagcttct tctctcattt tcaccaatcc ccctgttttt tcacatatat gatattttta
     2581 tatattaata cacataaatt caagttttta tatatattca tgtatatttt tcatgttata
     2641 tctattctac acctttatag tagtgtcctg caactgttcc aatttcatct aatttcttat
     2701 acaagtctgt agcctttttt ccaagtttat aattattact caatacctca atgtgagctt
     2761 caataacctt tttaaccatc tcactatctt caactgtaaa atctatccta aatacatcta
     2821 ttccaatttc ctctaactca tatagattgt ccaacataca agtaaccttc gaattgtaaa
     2881 ttgtacttct acagaatata tcttgtgtta tcctataact tgcatccgta aagtctctta
     2941 aagcataact actttcctta catttagcaa ctctcttgtc ctttttacaa tctctaacaa
     3001 ctactcccat aggacagtat tctgtaacca tcataggagc atatccatat attactgctt
     3061 caatttctac atctgtatat tttaaagttt gtttaatttc atttaagttt agttcttgtg
     3121 acaaacaaac tgtacttgct ccctcttctt tgaagtggtt tatactttca ctattgaatg
     3181 catttaagta gcaatctata tttaagtctt ttttcttaaa gtattccata gagccccagt
     3241 taccaatttg aaccttatta attcctttta tattatccaa tatctcaaat tgcttgtact
     3301 ctttatttct aacaatcctt ggtgctgaat agattaattt tttgttagtg actgctgtat
     3361 tatagtggtt cttattcaaa gtatctattt tacttgtatt tgacaaagta tctatgttat
     3421 ttgtatttga acccattagt tctttaggag tatctaaaga cgaagaagta tctaaatcca
     3481 tagaaatact tgaatctata gaaatatttg aatctataga aatacttgag accatttgta
     3541 aagcttttga taatgtattt atatcttcat aatagattaa atctacatta taatttaata
     3601 ctgcttctaa ttgctccaag ttcttaactt taactctaat cttaggaact gtatcaagtt
     3661 ttttctgttt atttactcta tttggctggt attttaatct attcttctta aattttctat
     3721 cttttatctt tattctttct tcatctaaaa gctcaataca atcccttctc atttgattta
     3781 acacacttat aggcatactt acaccttcat ccaggtcaat ttctatctct tttaacttat
     3841 atggtgtatt tccaagcttt tgaatttgtg tttctatttt ttctttactt aaagccacct
     3901 ttatagcttt ttcaactatt ttgtctcctt ctaccaccga tttatttcca caaaaatcac
     3961 ttataaaaag ctctggcttt ttacctagtt taatactcac ttttgcacgt atattagttt
     4021 ttatgaactc tttatcttgc tgaaatgtag cttgtactct atccatcaat tcgctgtcag
     4081 aagtcttata tataagctga tttctctttg cttctccaac aaaatctaat tctatcaaat
     4141 cccctttatg tgctattgta gttatctctc catcctttat tattcttcca atagtacctc
     4201 cacctaagtt aattccatca cctttcttaa gcgtattttc tagcattact ctaagtcttt
     4261 tgttctttct attataatct aaaacttttc caatatatag cccttgatta ttaggtctat
     4321 tagagttcat tatatcctta ccaacttcac ctaatatata tccttttgta aactttctat
     4381 taaatatggt atacaaatta tttattattt cctcagaaat acttattttt ttcttatcta
     4441 caaattcatt aatcgcttct ctatatcctg aaaccacagt agctacatac tctggctttt
     4501 tcattctacc ttcaattttt aaagataata cacctgcttc aattatttta tccaaatctt
     4561 ctatagtact taaatctcta ggactcaaca aataatctcc attacttttt actacttctc
     4621 ctgtatttat atctatcaac tcatactttt gtctacatgg ctgtgcacac ctacctctat
     4681 ttccagacct attgcctatc atactactca taaggcattg acccgagtaa catacacata
     4741 aagccccatg tacaaaaact tctatatcta cagttgtatt ttcacaaatc cattttatct
     4801 catcaacatt taattctctt gctaaaacaa ctctatcaaa ccctacactc tgaaggtatt
     4861 ttacatcttc taaggaatgt gctaccattt gagtgcttgc atgtagttca aagtcaggta
     4921 atagtttttt tataagtctt gccattccta tatcctgtac tattaaagca tcaatatcta
     4981 tatcgtataa aaattttaca tactctacaa aatcttctat ttcatactgt tttatcaatg
     5041 tatttacagt aacaaagact tgcactcccc ttatatgaga gtatttaaca gcctcaatca
     5101 gctcatttct atcaaagtta tttgcagatg ctctagcact aaagtcttta ccacctaaat
     5161 acacagcatt tgctccgttt tgaactgctg cttttaatga atcaaaagag cctactggtg
     5221 caagtaattc tatttttttc atatttttct cctctattta tacttgttag gttaatataa
     5281 ttatatctat aacttaaata tttgtattaa atttatatat ttcttactat attttaaatt
     5341 ctttaattaa tctatacaat atttgctata tataaagtat atcaaataac agcttcattt
     5401 ttttgtaaat ttacagaaag ttaattttca ttaaatgaat catctttatt gataaacata
     5461 attccaaaga ccactgccaa aataaaaaaa catattagta ttgatttttc cacgaaaatt
     5521 tttgtaaaaa aagttcctaa aatagctcca gatataaaga atataataat agcataatac
     5581 tgtaaactct tttttatcat aaaaatattc ttagtatgta tataatgaaa taaagtttct
     5641 gtagcacttc ttaaatttcc agtacacatt gtcgttgcaa aactattccc atttactttt
     5701 ctaaaactct ctacttgcat tgaacaaata aaagaaatag caacatttac aaacatatta
     5761 aatcttcctc taggaataaa tgaaacaata agtattgtaa tcatttcaat tataatgata
     5821 acctgacgcc aatgaatctt tgtcttatta tttgtaaaat actttcttat cttctcggct
     5881 ataaatacac ctaacataaa tgccaaaata ggaatcaaat aataaaatga ttcctttaca
     5941 ttaccttctg ctatattaag cccaaacaaa actatatttc cagtttgtgc attagcaaag
     6001 actttacctc ttaccacata agtataaata tccaaaaatc ctcctgttat tgccaacaaa
     6061 gaacataaaa agaatgattc agacatctga ctcttgcttg tcatatataa acctctcgct
     6121 ttctaatgat agatacaaaa tcaaaatcat tttagattct atttttcatt gatataaaaa
     6181 aattatttat atttaaatat tgtctatatt tatacatcat ttatatttat ttatatttaa
     6241 acattatcta tatctatata gcatttatat ttatacatta aaagaagctg tctcaaaaaa
     6301 caagagacag caaatctttt agtttattcc acattttttg catcattatt taaccctgta
     6361 cattttaact gtaaatcata agctttgttt tgaaactcag aagctaagtt ttcagctata
     6421 gtagctcttt catttgcttc ttcaagttga gtacttaact cctcaatctt gtttttataa
     6481 gcatctaact ctgctctaga accttctgtc gtattactaa gttcgaatat ttcttgcttt
     6541 ttattttcta taatctcaat taactctttc atcttagttt cagcttcacg attttctgct
     6601 tctttttctg ctatcgtaag ctttaaagat ttgatttcta attgaagctc ttcattagat
     6661 gctccaacct tcttactcaa ttcttcattc gctttaatca acttctcatt ctcatctgaa
     6721 cattcaaata aaaggtctga tatatttaat gcagttaata tcgcagcaac tgataagctc
     6781 aatttcgggt tttgacgtgt tattttatcc atttctttgt ctacaaaatc agcaatacta
     6841 atcataaact tctctgattt gtctccaacc attggatatt cagcgccatg gattttaacc
     6901 attactttgt tcatcctaac ctcccccgac attagttgtt aatttgatct taagttagca
     6961 tttaattttt cgcttaattc acttaatatc ttatcatgca ccttagctac atcttcatct
     7021 gttagagttt tatctttact tctataagtt attgaatatg caattgactt gtatccagct
     7081 tctatttgag aacctttgta aacatcaaat aacttgtagc tttctactaa tccttgacca
     7141 ttttccttta ttatatcttc tatttgtttt acaaatacat catctttaac aataagagct
     7201 atatctcttg atgtagatgg atattttggt aatggcacat aattctttgt tctatcagaa
     7261 ttatcaaata caaagtcaat atctatttca gcgacataaa ctctttgacc tagattgtag
     7321 ttttctataa catctggatg caattcacct aatgtaccta catatttatt attatacact
     7381 attttagcac atctaccagg gtggaaggta gtattattat cttgaggttc aatttcataa
     7441 cctttaaatc cgacatttac aagtatagtt tctatagttc cttttagtgc aaagaagtca
     7501 acatctttac catacatacc tacacacatt ctattttctt ccttaggtaa ttctgaatct
     7561 tgtgggatga atatatgtcc acactcaaat gcagatactt cttctatttt atgagataca
     7621 ttagttgata aaacatctaa catatttggt attaaagtag ttctcattac agaagtttct
     7681 tctcctaatg ggttaagtaa tttaacaaaa tttctctttg cattaccttc tggtactctt
     7741 atcttatcta ctcccttagg gcttacaaat gaatatgtta atatttcatt taatcctaca
     7801 gcagttgaat tatcttttaa agcatccatg aatttttgtt ttgaagtttt aattcctgct
     7861 gtcgtatttc cttctaattg tacagaaggt atattctcaa atccataaat tctagctatt
     7921 tcttcccata catcagcttc ttgttccata tctgttctaa agcttggtac atctatttct
     7981 aatttatcat tagctaccaa attacattta aactctaatg attctaaaat tcctacaaac
     8041 tgctccattg gtacatctac acctaatagg tggttaattc tttgaggatt tactaccaat
     8101 tttttaggtt ctggtttatt tggatataca tctacaacac cttttaaaac ttttcctgct
     8161 cctaacattt caacaagttg tgcagctcta tttgctgcta tctctgcaag attagggtct
     8221 aagtcttttt catttcttga agatgcttct gacctaatac caactttttt agctgtcatt
     8281 cttatgtttt ctggtttgaa attggcactt tcaaaaagta cagtcttcgt attagaagtt
     8341 atttctgagt tagcaccacc cattacacca gctaaaccaa gtgatttttc tccatttgtt
     8401 ataactagca tatctttgtc taatgttctc tcaacatcat ctaatgttac aagtttctct
     8461 ccatctttag catttcttac tactattctt ccagtctcta cttgatttat atcaaaagca
     8521 tgaagtggtt gaccaagctc taacattacg aagtttgtta tatcgactat gttacttata
     8581 ggtcttactc ctgcttctgt aagtcttctt tgcatccaat atggagaagg ttctattttt
     8641 acatctgtaa ccattctagc aacatatctt ctacataaat ctggattatc tatctcaact
     8701 ttgaaatcta tctcttcgtc actttctttt acttcgattt caggatattt tacttttgtt
     8761 cctatagttg cagctgcttc tctagctata cccatcatgc atttacaatc aggtctgttt
     8821 gaagttaatt caaaatctat taaagcatct tttaatccta aaacatcttt tatatctttt
     8881 cctaattcat aagaatcttc gtggtctaaa atatatatac cacctctttt atactcctca
     8941 atgtaatgtt catctatacc tagttcagct gctgaacaca tcataccatc tgataattca
     9001 cctctgaaat cagtctgttt gatttcaact cctccaggta acttagaacc atttacagct
     9061 actggaatat aatctccttc tgatatattt gtagctcctg taactatttg aacaacttta
     9121 tctccaatat ctactttggt tacaaccaac ttatcagcat ttggatgttg ttttatttct
     9181 aaaatctttc caaccaatat attttctatt tcttcaccat aataatctat tgtttcaact
     9241 ttagttcctg tcattgtcat tttatcagcg aactctttta catccatgtc tatatcaaca
     9301 tagtctctaa gccattttaa agatactaac atctacatac cctcctaatt aaaattgatt
     9361 taagaatctc atatcatttt cgaataataa tctaatatca tctatttcgt atttaagcat
     9421 tgcaagtctt tcaaccccaa ctccaaatgc aaatccactg taaacttctg ggtctattcc
     9481 acaatttcta agcacatttg gatgaaccat acctgaacct aatatttcta tccaaccttc
     9541 atatttacac attgggcaac ctttaccacc acatttgaaa caagtaacat caacctctgc
     9601 acttggttct gtaaatggaa agttgtgagg tctaaactta gttttgatat cagaaccaaa
     9661 caatttttca acgaagatat ccatagttcc tttaaattct gccatagtaa catcttttcc
     9721 aacaacaagc ccttctatct gatggaacat tggtgagtgt gtagcatctg gcgagtctga
     9781 cctaaaacat ctacctggtg caattacttt tattggtaac tcttgacttc tcatagttct
     9841 tacttgaact ggagatgttt gagttctaag taatacccca tcattgatat agaatgtatc
     9901 gctcatatct cttgatggat ggtctttagg agcgtttaat gcgtcaaagt tgttttcaac
     9961 agtctcaact tctggccctt ctgctataga gaatcccatt ccgataaata tatctgttac
    10021 ttcatctata atttgagtta ttggatgctt cttcccaacc tttaaaactt ttcctggttg
    10081 agtaacatct atcacttcct cttttaattt tctttctttt tcaatagcat ttatttcttc
    10141 ttttttggaa tttatgctaa gttcaatgtt ttctcttacc tcattggcaa ccttaccaac
    10201 tactggtctt tcttcagcag ataatttacc catttcttta agtatggcag ttatctcacc
    10261 tttttttcct aagtacttaa ctcttaaact ttctacactt tctatgcttt gtgcttcttt
    10321 tatttcagcc aaagctgctt cacgtaaagc aagtaatttt tcttgcacac atatcaccct
    10381 ttcgtttttt tgtataaaaa aagcccctca atccctgtct agggacgaaa ggctatagtt
    10441 tcgcggtacc accctagtag tttaacaatt taaaaatcat caaaccacct ctaacaaact
    10501 taacgcgttt ttaacgtcaa agcctactgt aaaatttcag ctctgttact ccagagggaa
    10561 cttctcatga cctcttagaa aacactccca gcctaggtgt ttatctcttt actaagttta
    10621 atcaaaatac tttcctcttt cattgcattt acaatattat attgtttatt ttagtattat
    10681 atcatttcca ttcaaattat gtgactattt ttaatatata tccaat
//
LOCUS       DAENCV010000125        10690 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAENCV010000125 DAENCV010000000
VERSION     DAENCV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.127"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            313..771
                     /locus_tag="KQD82_003101"
     CDS             313..771
                     /locus_tag="KQD82_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381556.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFEEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            832..1107
                     /locus_tag="KQD82_003102"
     CDS             832..1107
                     /locus_tag="KQD82_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG4381557.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1166..2440
                     /locus_tag="KQD82_003103"
     CDS             1166..2440
                     /locus_tag="KQD82_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG4381558.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2570..3622
                     /locus_tag="KQD82_003104"
     CDS             2570..3622
                     /locus_tag="KQD82_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG4381559.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DNHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3622..4653
                     /locus_tag="KQD82_003105"
     CDS             3622..4653
                     /locus_tag="KQD82_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG4381560.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     KESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4745..5557
                     /locus_tag="KQD82_003106"
     CDS             4745..5557
                     /locus_tag="KQD82_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG4381561.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5560..6480
                     /locus_tag="KQD82_003107"
     CDS             5560..6480
                     /locus_tag="KQD82_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG4381562.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6655..7101
                     /gene="rpiB"
                     /locus_tag="KQD82_003108"
     CDS             6655..7101
                     /gene="rpiB"
                     /locus_tag="KQD82_003108"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG4381563.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7131..7796
                     /locus_tag="KQD82_003109"
     CDS             7131..7796
                     /locus_tag="KQD82_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG4381564.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7906..9408
                     /locus_tag="KQD82_003110"
     CDS             7906..9408
                     /locus_tag="KQD82_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG4381565.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9720..>10690)
                     /locus_tag="KQD82_003111"
     CDS             complement(9720..>10690)
                     /locus_tag="KQD82_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4381566.1"
                     /translation="IIQYLVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKF
                     NYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNEMMK
                     AELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFE
                     ASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKT
                     WRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRG
                     DISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         4274 a         1206 c         1965 g         3245 t
ORIGIN      
        1 aataatttta taaaatagtt gttaaacata aaaaaccatt tgtaaaaaac aaaaaaatca
       61 tttctctaac aaaaaaaacc acaaaaaata taaaaaagta tggctagtta agtgattaac
      121 aagtaaaaat taaaataaaa atcaaaaaaa atttatcaat gaaagtcagc taaaatcctg
      181 gaagagagca ttatttttgg gaaaacattt tcttttgttt cacaaaaaaa gccacttttt
      241 ggcacacaac atgctttata taaagtcaag aaaaactaag gtataagaca aaggcaatta
      301 ggaggtgtaa atatggagtt tataaatttt gtaagtgata aagttataga ccttaaaatg
      361 gattgtaaaa cgaaagaaga tttctttgaa gaaatacata ataaagtttt tgatttaggc
      421 tttgtaaaag aagaatttgg agaaaaaata ttagcaagag aaaatgtatt tccaacaggt
      481 ttaaatttag gagattatgg agtagcaata ccacatacag atgcagaata tattaaagag
      541 caatttatat cagtctgtac ttttaatgaa ccagtagtgt ttagttctat ggaagaccaa
      601 gatgaaaaag tacctgtaaa tttggctttc gtattaggac ttaatcaacc acacagtcaa
      661 ttatctgtat taacagaatt aatgggaatt atgcaaaata aagagttagt tgataagttg
      721 atgagttcta ctgataaaga agaagtttta aagacactaa agtctttata aaaataaaaa
      781 agtactaaaa ataaaaaata taaaaataaa aattattagg aggaaataaa aatgaaaaaa
      841 atattagtag catgtggagc aggaatagca acatcaacag tagtttgtga caaggtagag
      901 agattagtta aggaaaataa tatacaagca gaagttatac aatgcaaaat agctgaatgt
      961 gcaacaaagc aagaaggagc agatttaata gtatcaacaa ctatactacc aacaacttat
     1021 aatataccag caataaaagc aacagcatat ataactggag taaatacagc agcattagat
     1081 aaaaaaatat tagatgcttt aaaataatcc tatcatagat acattgaaat aatattttat
     1141 aaaacagaaa aaggaggatt tataaatgga taaattatta gcagtagtac aatacatttt
     1201 aaatatggga ccgacagtaa tactaccaat aacaattgta gtactaggaa tgatatttag
     1261 aattaagttt acaaaagctt taaagtcagg tcttacaatt ggtataggat ttgtgggtat
     1321 aaacttagtt gtaaacctat taacatcaac actagggcca gcggctcaag gaatggttga
     1381 aagattcgga cttaatttaa cagtaataga tgttggttgg ccaacaacag catcagcaac
     1441 atgggcttca ccagttgcac caatattaat accagtttgt ttattagtaa acttactatt
     1501 attatacttc aataaaacaa agatattaaa cgttgatata tggaattatt ggcactttat
     1561 agtagcaggt gcagttggat atatagtaac aggaagttat gtttgggcta tattctgtgc
     1621 aatactaatg gagttattag tactgtttat tgcagaaaag acagcaaaaa atgttcagga
     1681 gttttatgat ttagaaggta tagcattacc aactggttca acagcttcat ttgctccact
     1741 tggatattta gtaggtaaat ttgttgaaaa aataccagga ataaataaaa tacaagcaga
     1801 cccagactct atacaaaaaa gatttggtat atttggagaa ccaatgatga tggggattat
     1861 actaggtata atacttggag cacttgcggg atatgatgta gcaggaatat tcaatgtagg
     1921 tatatcaatg ggtgcagtaa tgttacttat gcctagaatg gttagaatat taatggaagg
     1981 tttaattcca atttcagaat caataagaga tttcttacaa gcaaaatatg gagacagaga
     2041 actttatata ggtctggatg cagcagtagc aactggacat ccagcagtta tatcaacagc
     2101 tcttatactt gttcctataa ctttgttctt agcagttata ctaccaggaa acaaagtttt
     2161 accatttgga gacttagcga caataccatt ttatatggca tttatagtgt gttttagaaa
     2221 aggtaatata gttcaatcag ttataacagg aacaatagta atagctatat cactttatat
     2281 ggctacaaac tttgcagatg ttcatacaca attattacaa caagcaaata tgatgccaga
     2341 aggaacaaca atggttacaa gtatagatac aggtggaaac ttgctaaaat ggggaatatt
     2401 gaaattatct caattagttt cttcatttgg aatattctag tatgtagtta atagagatta
     2461 ctctttttta gtaaagtatg cgaaaattta acatttagca gctactaaaa aagagtaata
     2521 ttttttataa atttttaaaa atacaaaata caagaggagg agatttatcg tgaaagcagc
     2581 agtattacat ggcacaaatg atatgagatt tgaagatata gaaataaaac catgtgaaag
     2641 tgatgaagta aaaataaaag ttatggcagc aggaatatgt gggtcagatc ctccaagagt
     2701 attaaaacat tggaaatatc cagttccagc tataccagga catgagtttt ctggagtaat
     2761 tgctgaagtt ggtaaggatg taaaaaatgt aaaagttgga gatagagtag ttgcaattcc
     2821 ttttatacct tgtaatgaat gtgaatattg taaaagagga ttattttcat tgtgtgataa
     2881 ccatggaatg ttaggagcta aaagttttgg agcttttgca gagtatgtaa atataaaagc
     2941 tacaaatgta cttccaatag gagatatgaa ctttgaagat gctgcaatga tagagccact
     3001 agcagttgct atgcatggag tacttaatat aggtgttcaa gttggagaca ctgtagctgt
     3061 aatgggtagt ggaacaatgg ggcagttggt aatacaagga cttaaaatag caggggcagg
     3121 aacaattata gcagttgata tatctgataa taaattaaga gaatcaaagg aattaggggc
     3181 tgatataata attaatgcta aagatattaa cccagtagaa aaaataaaag aacttactgg
     3241 tggaaaaggt gttgacatag ctcttgaatg tgcaggttca aaaataactc aagaacaatg
     3301 tttacttata actaaaaaga aaagtaagat aggattttta gggatagcat attcagatat
     3361 aactttatca gaagaagcat ttgaaaatat atttagaaaa gaactagaat taaaaggttt
     3421 ttggaactct tattctgcac catttccagg acaagaatgg actaagggaa tcaatctagt
     3481 taatgaagga aagataaaat taaaagaaat ggtatctcat agattctcac ttgaagatac
     3541 ttacaaagca tttgaaatga taagagatag aaaagaagaa tttaacaaaa tattaatttt
     3601 accacaaggg gttgaaaaat aatgaaagca gtagttaaaa ctaaaccagg ctatgataat
     3661 gtagaagttt tagaagtaga agaaccaaaa gctacaggag acaaggttaa aataaaagta
     3721 gaatatagtg gtatatgtgg ttcagatata cattcattta aaggtgaata tgcaaatata
     3781 aaagccccag taacattagg tcatgaattt tctggaattg ttgtagaagt aggagaagat
     3841 gttaaaaata taaaagtagg agatagagtt acatcagaga ctacttttgc aacttgtgag
     3901 aaatgtatat attgtgaaac taaagattat aacttatgcc caactagaaa aggtattggt
     3961 actcaagtaa atggaagttt tgctgagtat gtattatcaa gagaagaaag catacatgta
     4021 cttcctgaaa atgtatcttt attatcagca gcccttacag agccactagc ttgttgtgtt
     4081 catgcagctc ttgaaaagac tactatagaa agttctgata cagttttaat aataggacca
     4141 ggaccaattg gtttattatt agctcaagtt gttaaatctc aaggtgctac agttataatg
     4201 tctggtgtta caaaagataa ggaaagatta gaaatagcta aaaatcttgg agtagataga
     4261 actgtaaatg taatgaaaga aagtttggca gatgtagtga atgaaatgac tgatggaaat
     4321 ggagtaaaca aaggatttga ttgttctggg tttatgccag cagttaacga agccttaaga
     4381 ctgcttagaa aaaaaggtac tttcgtacaa gttggaatct ttgctaaaaa attaaatgaa
     4441 atggaccaag aagcaataat ccaaagagag ttacaatata taggaagcag gtctcaaaag
     4501 ccaagttctt ggattacagc attagattta atggcaaatg ggaaagtaaa tacagaagct
     4561 ttagttacta gaactgttgg tttagacgaa tttaaagaag gtattacagc tttaatgaac
     4621 ggggaagaaa taaaagtagc agtaaaatct taaaaaaaca tttgtattat tttaaaaaaa
     4681 attgtaaaat ctactgtagg ataaaaaaat gtaaggtctt aaaaaataac aggaggaaga
     4741 aagtatgagc actcttagag atcattctaa taatattaga aaaaacataa taaaaatggt
     4801 tgcagaagct aaaagcggac atccaggtgg ttcattatca atagctgata tgttgacagt
     4861 attatatttt gataagatga acgtatgtgc agaaaatcca aaaatggctg atagagatag
     4921 atttgttcta tcaaaaggtc atgcagcacc agctttatat gctacattag cagaaaaagg
     4981 atttttccca gaagaagaat taataacact aagaaaattt ggttctaagt tacaaggtca
     5041 tccagacatg aaaaaagtag caggaatcga tatgtctact ggttctcttg gtcaagggct
     5101 atctgcagca aatggtatgg cacttgcagg aaaattagat aacaaagact atacagtata
     5161 tactatatta ggtgatggag aaatccaaga aggtcaaata tgggaagctg caatgacagc
     5221 atcacattac aaattagata atttaattgc atttgttgac ttaaatggat tacaaataga
     5281 tggttctaat gaagaagtaa tgaatgtaaa tccagtagat gacaaattca aatcttttgg
     5341 atggaatgta atagtaataa atggacattc ttttgaagaa ataggaaatg cagttgatga
     5401 agctaaaaaa gttacaggaa aaccaactgt aataatagct aaaacagtaa aaggtaaagg
     5461 tgtttcattt atggaaaata atgctgcatg gcatggaaca gctccaaatg cagaacaagc
     5521 agagcaagca ttgaaagatt tagaaggagg tctataggaa tgggaatagc aactagagaa
     5581 gcttacggac aagtgttaaa agaacttgca gaaaataaag atatagtagt attagatgca
     5641 gatttaggaa aagctacaaa aagtatatca tttaaagaag ttgccccaga tagatttttt
     5701 gacatgggga tagcagaagg tgacatgatt ggtacagcag caggtcttgc tacttgtgga
     5761 aaaatacctt ttgcgagtac ttttgcaata tttgcagcag gaagaggcta tgagcaaata
     5821 agaaactctg tagcatatcc aaatttaaat gtaaaaatag cagctactca tgcaggtgta
     5881 acagtaggtg aagatggtgg tagccatcaa gctatagagg atatatctct aatgagaggt
     5941 ataccaaata tggttgtatt aaatccagca gatgctttag aagcaagaca agctattctt
     6001 gcatctattg attataatgg acctgtttat ataagattag gtagagctgc aactcctgat
     6061 gtaaacagtg aaaattataa atttgaaata ggaaaaggaa ctgttttaag agaaggttct
     6121 gacataacag taatagctac aggaataatg gtagcaaaag ccttagaagc agcagaagaa
     6181 cttgctaaag aaggtataaa tgtagaagtt gttaatatat ctacaataaa accattagat
     6241 gaagcattaa taaaagaaac tgctaagaaa actggaaaag tagttacagc agaggagcac
     6301 agtataatag gtggtttagg ttcagctgta tgtgaagctt tagcagaaac taaagatgta
     6361 gtagttagaa gaattggtgt taaagatgta tttggtcaat caggtacacc agctgattta
     6421 ttaaaacact atggtttaac tacagaagat atagtaaaaa atataaaaga gttattatag
     6481 atataaatat atctaaataa caaattgatt tgacataagt caattaaagc tgtcttaaaa
     6541 atagaaagaa atttctacaa tgggacagct ttatttttac aatactttat ttttatgata
     6601 attataaaat agtctgaaag taatcaaaaa aattataaaa agaggtgcta tttaatgaaa
     6661 atatctattg gatgtgacca tggtggttat gaattaaagg aatatataaa ggaaaattta
     6721 ataaataaag gtatagaagt tgaagatgtg ggtacaaatt caaaagaaag tgtagatttt
     6781 ccaatatatg caaaaaaggt aagtcaaaat gttcaaagta aaaaatctga tttaggcata
     6841 ctttgttgtg gaacaggaat aggtatgtct attgcagcaa ataagtttaa aggaatcaga
     6901 gcagctgttg tttctgactg tttttcagct aaggcaacta gagaacacaa taatacaaat
     6961 gtactttgtt taggagaaag agttattggg aaaggattag caatgagaat tgttgaagaa
     7021 tggttaaatt caagttataa tggagaaaga catgcaagaa gattacaaat gattgatgat
     7081 atagaaaaag aaaatttata aatatataat ttataacaag ggggagcgtt atgtctatta
     7141 tttgtgcttc aataatgtgt gcagaccaaa tgaagttaaa agaggaatta gaagctcttg
     7201 aagaagcagg tgtaaaatta cttcattgtg atgttatgga tggaatattt gttaaaaact
     7261 tggctatggg accagagtta ttaaaatcaa taagtgaaaa tacaacaata cctttagaca
     7321 tacacttagc cacagaaact ccagataaat atattgatat gatgtcttat ataaagccaa
     7381 aatatatttc ttttcatgta gaatcaagta caaatgtaaa agctgatata caaaagttaa
     7441 gaaattatgg tatagggcca gtgttagcaa taagccctca aacaagtgtg gataaaatag
     7501 aagaatatat aagtttagtt gagggtatct tagtaatgac agtaaatcca gggtttgcag
     7561 gtcaaaagtt taatctttct gtattggata agttggataa acttacagag atactaaaag
     7621 attatgataa tcctccattt atagaggttg atggaaatat aaataaggat actattaaac
     7681 ttatgaatgg taaaaaagta gatatatatg ttgttggtac ttcagcttta tttaatgata
     7741 agcctccaat ttcatataaa gataaaatag aagaactaaa agaaagtata aagtaaccat
     7801 gtaataaaat tataatatgt ttctgtttaa tagtaaaaat ttaggtggaa acaagaaatt
     7861 ttataatatt taataaaaat tttggttgag taaggagaat tttttatggg agacctatat
     7921 aaactacaaa atggtactga tattagagga atagcgtatg aaaatgattc taaagaagtt
     7981 aacctaactg tagaagaagt aaaaaaaata gctaaagcgt ttcatatttg gttaaaagaa
     8041 aagactaaga aagataaagt aactgttgcc ataggaactg attctaggat aacaggaagc
     8101 caatttagaa gtactgttat tgaaacttta actaatgatg attgtaatgt aattgattgt
     8161 gagattgcca caactccagc tatgtttatg acaactatta tggatggata caattctgat
     8221 ggggcgatta tgattacagc aagtcactta ccttattact ataatggttt aaagtttttc
     8281 acagaaagtg gaggtttaga aaaaactgat ataaaagaga tgttggatat agctgttaaa
     8341 aatgatagtg tagactatga ggaagtaaat aaaaaaggtg aagttatagt aaaaaattta
     8401 atagaagatt attctaattt attgatagat aaaattagaa aaggtgtaaa ttcaagtaaa
     8461 aattatgaaa agcctttttc tgggcttaag atattggttg atgcaggaaa tggggctggt
     8521 ggattctttg ctgaaaaagt tttacacata ctgggagcag atacgacagg tagtcagttt
     8581 ttgaacccag atggtatgtt cccaaatcat atacctaatc ccgaaaacaa agaagcaatg
     8641 gaaagcatat gtaaagctgt gttggataat aagagcgacc taggaataat ctttgataca
     8701 gatgtagaca gagctgctat tgtaggtaaa aatggcaaac caataaataa aaatgctctt
     8761 atagctgtta tatcatcaat agtattagaa gaacatccaa aaacagctat agtaacagat
     8821 tcgattactt ctgaaggact tgctaaattt ataaatgaat tacaaggaag acatcataga
     8881 tttaaaagag gatataaaaa tgtaataaat gaagctataa gattaaatag tgaaggtgaa
     8941 gagtgccatt tagctataga aacgtctgga catgctgctt taaaagaaaa ttatttctta
     9001 gatgatgggg catatttaat agctaaaatt ttaataaaag tagcaaagct atccttggaa
     9061 ggaaagacta tagaagaatt gatagaaaac cttgaagaag ctcaagaaga gaaagaagtt
     9121 agaataggca ttaataaaga agattttaga acatatgcag aggatatatt aaaagattta
     9181 gattctcatg ttaagagtat tgatggatgg tctgtagcac caaataattt tgaaggcata
     9241 agagttaatt gtgatgaatc aaatggagat ggatggtttt tacttagaat atcactacat
     9301 gaaccattat tagctttaaa tattgaatca aataaaattg gcggagcaga acaaatttat
     9361 tctaagttaa aacttttctt agaaaaatat gatttaaaag gtctttaggg taaatagaaa
     9421 gaatatattt atattgaaga ataaatgtaa atttgtacct aagattacaa atattacaaa
     9481 attgtaatct taggtaacag agatgtaact gattatggaa acactatgtg atattataga
     9541 tacagagaga tgaaatatgt gatgaattat agagaaatat aatttaaaat gtattaatat
     9601 cacaccaaaa aggtataatc aaaaaatatg taaatataaa ccccaacatt tagaaaaaga
     9661 cctctaagga ggtttttttc ttgtttaaat aacttaaaat atatttttat attaatatat
     9721 caattaactc ttctaaaagt aagcattacc ttaaataaat cagcatctat aattatttcc
     9781 atgtgtgcat tttctaattc aattatattt tgagcaattg ataaaccaag accagagcca
     9841 tctgtttttc tagatatatc accccttcta aatctttcta ttaactcttc tggttttaga
     9901 tttagtttat catttgaaat atttttaaac gttaatatta tattttcttc ctttataaac
     9961 atatctatat agaccctagt gttaggcata gagtatttta gtatattgca aattagattc
    10021 tcaaaaactc tccaagtttt tttaccatct atcatggtaa aaacagcata ttcaggtgtt
    10081 tctattataa aatctaaact gctattttga attttgtctt caaattctac aatactttga
    10141 attaaaagtt ctcttatatc ggtttttgtt atattaaatt ctacatctcc actacttagc
    10201 ttagatgctt caaatatgtc atctattaat aattttaatc tttgtgattt tttatctaaa
    10261 atatttatat agtcatttat agtctctctg tcaaagtcat tattttttaa catatcaaca
    10321 tagcttaata tagatgtaag tggagttttt aagtcatgag atacgtttgt gataagctct
    10381 gccttcatca tttcattttt tatattagca tctacagcag tttcaagacc gcctgttata
    10441 ttatttatac ttttggctaa agtagatatt tcatcatctc ctataacttc taatttatac
    10501 tttaaattgc cattttctat attttttata ttttttgata tgttatttat gtaattaaac
    10561 ttaacagata aaaagttgta tatataaaaa tgaaaaactg tgtttacaag tgtaaataaa
    10621 attactatag ctattgcctc agcataatat atatacataa agaatatcaa aacaagatat
    10681 tggataatag
//
LOCUS       DAENCV010000126        10615 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAENCV010000126 DAENCV010000000
VERSION     DAENCV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.128"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            135..1271
                     /locus_tag="KQD82_003112"
     CDS             135..1271
                     /locus_tag="KQD82_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381567.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLAVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            1264..2811
                     /locus_tag="KQD82_003113"
     CDS             1264..2811
                     /locus_tag="KQD82_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG4381568.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            3145..4011
                     /locus_tag="KQD82_003114"
     CDS             3145..4011
                     /locus_tag="KQD82_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG4381569.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            4152..5294
                     /locus_tag="KQD82_003115"
     CDS             4152..5294
                     /locus_tag="KQD82_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG4381570.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            5678..6916
                     /locus_tag="KQD82_003116"
     CDS             5678..6916
                     /locus_tag="KQD82_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381571.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            6945..7832
                     /locus_tag="KQD82_003117"
     CDS             6945..7832
                     /locus_tag="KQD82_003117"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG4381572.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            7866..8495
                     /locus_tag="KQD82_003118"
     CDS             7866..8495
                     /locus_tag="KQD82_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG4381573.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            8500..8964
                     /locus_tag="KQD82_003119"
     CDS             8500..8964
                     /locus_tag="KQD82_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG4381574.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            9214..10581
                     /locus_tag="KQD82_003120"
     CDS             9214..10581
                     /locus_tag="KQD82_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381575.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         3951 a         1124 c         1808 g         3732 t
ORIGIN      
        1 ttgtagcaat attcagacaa atagtgacct aaaaatcgta ctttgtttac ataaataaaa
       61 tagtatgata taattataaa aacaacttat aatattgaag aaggttgctt gttcgaaaaa
      121 ggggatgata aattatgata gagataagaa atgtaacaaa aaaaatagga aataatgtta
      181 tattagatga tatttctcta gctgtagaaa caggtacatt ggtagttttg attggttcaa
      241 gtggttgtgg aaagactact actttgaagc ttataaacaa attaataaaa cctacatctg
      301 gtgagatata tattaatggc aaaccaattt ctcaggaaaa tgaaatagag cttagaagaa
      361 aaataggata tgttatacag aacacaggcc tattccctca tcttacaata aaagaaaata
      421 tagagctaat tccacgccta aaaaaggaaa aatccgttga agaaattgaa aaaagaacgt
      481 tgcaactact tgagatggta ggacttgata gtgatgaatt tttgaataag tatccttcag
      541 aacttagtgg tggacagcaa caaagaatcg gtgttgcaag agccattgct actgatgctg
      601 aaataatatt gatggatgag ccttttagtg ctttagaccc aataacgaga acatctttac
      661 aagaacaatt attttctctt caagatgaat tgaaaaaaac tataatattt gtaacgcatg
      721 atatggatga ggctttaaag atagctgata aaatttgtat tatgaaggat ggaagaatag
      781 cacaatatga tactcccgaa aatatactta gaaagcctgc aaatgacttt gtaaaagatt
      841 ttatcggaga agatagagtt tgggataatc cagaatatat aaaagcaaga gatattatga
      901 taaaaaatcc tattgctgtt aattctacta gaactgtaac tcaaggaata gaaattatga
      961 gaacgagtaa ggttgatagc ttactaatta tagatagagc aaaaacatta aaaggtattg
     1021 ttactgtaaa agatatgaaa gatattgatg ataagtcaat attacttgct gatattatgt
     1081 caagtgagcc tttgcatgta aatgaaggtg ataatttagt agaaatacta aatgtaatga
     1141 atcgtaattc ggttggatat attccggtta tttctgatga aaataaactg gtagggctta
     1201 ttactagaag tagtttactt tctgttttaa gtgaacaatt cttagaaatg gaggtgagtg
     1261 ttcttggcta atttttttaa ctttatacta ttacaaaaag ataagattat agagctttta
     1321 atacaacata tgagtctaac tgttacatct attttaatag ctataatagt tggtgttcct
     1381 cttggaataa ttatttcaag aatctcatct cttagaaagt ttgtacttgg atttgtaaac
     1441 ttagtccaag cagttccgtc tatggcactt ttgggtcttc ttgtacctat acttggtata
     1501 ggaagtaaac cagccatatt tatggtagtg gtttattcat tactaccaat tgtaaaaaat
     1561 acgtatatag gtataacaag tattgaccca gtagtattag aatcagctaa aggtattggt
     1621 cttacaagaa atcagacatt atttaaaata caattccctt tagcacttcc tataataatg
     1681 ggtggagtta gaatatctgc agttacagct gttggtttga tgactcttgc agcatttata
     1741 ggtgctggtg gtttaggata tttagtattt tcaggggttc aaactgtaaa taataatatg
     1801 attttagcag gggcaatccc atcatgtata attgcactgt tagttgatta tttatttagt
     1861 aaaattgaag ttgcagttac tccaaaagga ttaaatccaa aagcacctaa gaagaattat
     1921 atagcactta aagtaatatc tgtagtggtt gttgtttcaa tgttgttcgt tgtttttagt
     1981 tctagttttt caagtaaaaa agatacaata actattggtt ctaaagatta tacagaacaa
     2041 cttatacttg gcaatgttta tgctgaatta gtagaaaaaa atacgaatct taaagttaag
     2101 aagaatttaa atctaggtgg ttcatctgtt gcatttaatg caattaaatc tggtgagtta
     2161 gacatgtatg ttgactatac aggtacactt ttggtaaatg taatgagaca tgctccgatt
     2221 aaagatgctg atgaagcata taatgttgta aaagatacta tggaaaaaga aaatcagtta
     2281 actttacttg accctcttgg atttaataat acttacacat tagccatgat gcctgaaact
     2341 gctgaaaagt atggaataaa tactatttct gatttgacta aatatggtaa agaatttaca
     2401 ttctcaccaa ctttggagtt tgaaaataga gaagatggtc ttgtagggct ttctagagat
     2461 tatggtttaa aattcaaaga tgtaaaagca atgaatggaa gtttaagata tacagcttta
     2521 gataataatg aatctcaggt tatagatgca tttttaactg atggtcttat taaaaagttt
     2581 aatcttaaga tattagagga cgataaaaat ttctttgtac catattatgc agcaccatta
     2641 gtaagagaag atactttgaa gaaatatcct gagttagaaa aagttatgaa tatgctttca
     2701 ggaaaaataa atgaagaaac tatgagagaa cttaattatc aagtggatga acttggtaaa
     2761 tcaccagaag aagttgctca ttcattctta gttaaagaag gcttagtata aaaaattaat
     2821 agtttatatc aaaaatttaa gggagtgtct caaaatgaac tttttagttc atgaggcact
     2881 cttttttata tgaaatcaaa tatattttat catttcaaca ataaaaaaga agattatctt
     2941 tattttgaga cagtctctta tttttatgta taccaaaacc atacctacga tttttattta
     3001 taattaagtt tcaaatagaa tgaacaagta caaccaattt tgaatataaa tatagtaaat
     3061 gtgcttatat acgattttat tgtctggttg attgatattg atagatagct ttctttattg
     3121 aatcgaaggg agggggaatt tgttatggat aactataaga aagtgaagca agtattgtgg
     3181 ataattttat ttgctaattt tgcagtagca ttattaaaaa tcattattgg taatcaaata
     3241 aaaagctata gtatgacagc agacggattt cattctttgt cagatggagc atctaatata
     3301 gtggggctta taggtatttt ctttgcatct aaaccaaaag ataaaaatca tccttatgga
     3361 cataaaaagt ttgaaattat aaccagttta tttataagtg gtatgttgtt tgttatagca
     3421 attaagataa ttcttagtgc agtgttaaga atagctaatc cagttgtacc tgctataact
     3481 atagaaagtt taattgcttt aattattaca ctttttataa atatttttgt atgcatgtat
     3541 gaatatagaa ttgggactaa actaaatagt tatgttttga tttcggattc attgcataca
     3601 agaagtgata tatttgtgtc tctaggtgta ttggttactt tagttggtgt taagttagga
     3661 tttccagtaa taatagaatc tatagttcct attattattt ctgcattcat aatttattcg
     3721 gcttatggaa tatttaggcc atctattgga attttggtcg atagagtggc tgtagatgag
     3781 gattatataa aagaaattgt gtttgaattt aatgaggtaa gggatgttca caatatcaga
     3841 agtagaggta gcaaaagttc tatttatatt gacatgcatg taatggtaga cccatttata
     3901 agcgtagagc aatctcatga tttaacacat aaaattgaga aacaaataca agaagaaatt
     3961 aatgaaaatg ctcaagtaat agtccatatt gaaccttttt atagttttta acttagttat
     4021 aaaatacaag ttataaaaaa atataattta taaaaaaaat attataccat aaataattta
     4081 taaaaattat ttatggtata atattttcta taaagaaatt aagtcttaat atatctaagg
     4141 aggaatttac aatgagatac gttgaatatg gaaagacagg gaaaatggtt tcagtagttg
     4201 gctatggagg attaaggttt gatttagaaa aaagtaatca agaaaatgct gatttagtca
     4261 aatatgcgta tgaaaaaggt attaattact ttgatacagc accagggtat tgtgatgaca
     4321 gaagtgaaga tatatttgga ctagccttta gacaaatgat aaaggaaggt aagaccgatt
     4381 tttatgtatc aactaaagaa aagccaaaag catgtgatac atcagaaaaa gctatagagg
     4441 ctgtaaaaaa atcaatagaa cgattaggcg tatctaaaat aaatttttat catgtttgga
     4501 atattagaaa gatggaacat tacgaactag caatgagacc tggaggtcaa tatgaaggat
     4561 tattgaaatg taaagaagaa ggattgatag accatatagt attttcatct catcaaccag
     4621 gagatgaggt tatagatatt ctaagtgaaa ataagtttga aggtgtaact atgggaataa
     4681 atatattaaa ctttccatat aggctaaaag gtgccaaata tgctgttgat aatggatatg
     4741 gtgttgtggc aatgaatcca ttaagtggtg gaactattcc aaagtataat aaagaacttt
     4801 cttttctatc aaaagatggc gaaagtacta tagaatcagc tttaagattc aacataggaa
     4861 tttcacaaat aagtatatct ttgatagggt ttaataaaaa acaagatata gatgatgcat
     4921 gtaaaattgc agacgaaaat agaatttatt cagatgagga tatacatgat attgagacta
     4981 gattaaataa gaatatgaac gaaatatgta caggttgtgg ttattgtaaa gtatgtccaa
     5041 aaggtattaa taccccagcc tatatgcttt tttataatga aaagcagatg tttaaaaaga
     5101 gtgatgaaga aatgacaaag ttagtttatg gcttaggaca ttggaattac acaatgaata
     5161 gtaaggcaaa agctaaagaa tgtatatctt gtggaaaatg tgaagttgaa tgtactcagc
     5221 atttacctat aatagataga ttaaaagaaa ttaaaaaatg ggaagaagat ggagctaata
     5281 gtgttaaagt ctaattaatt ttatactata tttgaaattt gtatagtatt tagtaatgtt
     5341 ttaaaatata gttttaatat ttaaggagac agattataat tttgtctcct taaattatgc
     5401 tttaatttgt tatagtttaa ttccataagc aacattgtat atttttatat aaagtttttc
     5461 aaatatttta cattaatatt gcttacaaat tctcatttta tacaacacta atctatgtta
     5521 tataaacaat acttgactga ttataacatc aataaagtaa atattgtggt tgtaaataaa
     5581 gagttattca attacaataa aacaagtaag aacattatga agatggctat taaatataag
     5641 gaataaattg taggtgtcag caaatcctat ataatgaatg gatggtgata tattgatgag
     5701 taaaatatta aaatataaaa acgagatgtt tttgtttata ttagttgtaa taacttactt
     5761 aataataact aagatatttt tttctaagac tactatattt tatgatttaa acaatactta
     5821 tgatgtattg ttagatacag atacaggagt tttattcaat ttaaatgtat ttgctataag
     5881 tcaagacaat agtaagcaca tattgttttc agcaattata tcaatttttg catatccaat
     5941 atacctattt tgtacttcaa tagcaaatcc tgggacaaca gattttaata gtgcctatgg
     6001 gtttggacta atttgtctac aaataataac tagtgctatg tcaataactt tagtctttaa
     6061 tcatattaaa aagataaaaa tgcagagatt aactttaatt ttacttacta tgataatgat
     6121 attttctttt ccacagttat ttatgacttt aaatgtagag agatttatat atagccaatt
     6181 ttcattaata tttttcatag ttattgcaaa taagatgaaa ggaaaaaatt cttatttagt
     6241 agaattagca gctattcctc tatttggaat aactattagt aatatatatt tgtatttttt
     6301 taatatgata tttgagttta aattaaaaat aggaaaaatg ttaaaacatt taattacatt
     6361 tatcctaatg gcatatattt gtgtagtttc aacaaagtct tatgagagtt ttatgaacct
     6421 tggaaatgta atacagtatg atacgaaatt tataagtgga gaaccaattt tggaaaagat
     6481 tgcaatgatt atagagagat tattgtattc ggttttttat tttcctggag caagtattaa
     6541 aaaagggtta tttttgcaaa atggagaagt tgctacaata ccagtaattc ttacattatt
     6601 agctttatgt ttttgtgttt tatcagttat tgaaaattca gaaaaaagag ttcctaagct
     6661 atgtatggga ataattatat tcaatctaac tttacatggt atagtaggat ataatctagt
     6721 caattcaagt attatggcta taaattttag ttttgcagtt ataattttat tggcttactt
     6781 tacgaaagcg cttagaaaaa atgaaaagaa tatgtataat atatttctat cgctgttatt
     6841 ggttactata gtaatcagta atgtcaatgg atttatagaa attttaaaca tagggataaa
     6901 atcatatcct gtttaaaaaa taatattgaa agggtagtaa gtttatgaaa cagaccaata
     6961 caagtataag gcttcttact aaaatttcag attatataaa gttaatgaga ataaagcaat
     7021 atataaagaa tttctttgta ttttcagcta taattttttc aaacaacata ctaaatataa
     7081 atttattttt aaatacgttt attgcgtttg tgtgtttttg tctaatgtca tcatcagtat
     7141 atgcactgaa tgatgctata gacatggata aagataaaaa acatcctaaa aaatgtaata
     7201 gaccagtggc aagtggaaga ataagcaaaa aaagtgctaa tatactattt gtagtattag
     7261 ctttaatttc agtctgttta tcaacagttg ttagttttaa tctttctata attctcagtc
     7321 tctatttgat aaacaatata ttatattctc ttaaactaaa gaatataata ctattagatg
     7381 tgttttcaat atctcttggt tttatactta gggtttatgc aggatgtgta gccataagtg
     7441 ttagtttatc aaattggata attttatgta catttttctt atctctgtac ttagctcttg
     7501 gaaaacgtaa aaaagaaata gaaactttaa gagatgatgc agttgaacat agaaaaatat
     7561 tggaagatta tgacatagaa aatctaaatc aaatgatgat agttatatta agttctacaa
     7621 ttgtatgtta tgcattgtat tctacaagta atccagaaaa gccacatatg atatttacaa
     7681 caatatttgt agtttatggt gtacttagat ataattatat aattaacact acaaatgaaa
     7741 ataatcctac tgatattgtc ctaaaagata atgctttaaa aataaacgta atcttatgga
     7801 taattacatg tttaataata ttgatttttt agtgatattt ttagtgatag gatggggaaa
     7861 tacttatgaa aaaaagtgta ttattacttg atgtagatta tactgttata aatactgata
     7921 gcatgataga tttttttatt tactctttaa aaaataaaac ctttaaaact attataaagt
     7981 tgccatacat tatttttatt ctatttatgt atttaattag aatgatacct ttgaagaagg
     8041 caaaagaggc aatcttttat ccaatagtag attttagtga agaagattta aaaaagtttt
     8101 ttgatgattg cataatgaaa aaaattaacg aatctatgaa aaaggttatt tacaaaaata
     8161 aagaagaaga taatgttata attatgataa ctgcatctcc atatgcatat atgaaatatt
     8221 ttaagtatta tggtcttgca gacgaggtta taggcacgga gtttttttat gaaaattcaa
     8281 gatataaaaa tcaattcatt ggagaaaact gtaagggaat tgaaaaagta aaaagaataa
     8341 aggctgtatt aggtaaactt gggatagaga ttgattatga aaattcatat gcatattcag
     8401 actctaagag tgatttacca atgctttcat tgactaaaaa tgcattttta gttagtaaaa
     8461 aggatggtga ggttatagag caaataaatt cataacttaa tgtcacttag aataggaggg
     8521 aatagtttgg gatatttaca catattaatt ttatctatgg ttcctgtaac agaattgagg
     8581 ggagccattc ctataggtat tgcaatgggc ttaaatccta tatgggttta tgtatttagt
     8641 gtaataggtt ctacattggt atcgattccc ttgatattga cattcagaca tgtattgcaa
     8701 tttttaagag ggaagaaact atttaaaggg attgctgatg taattgatag aaaaataaac
     8761 tcaagaatga agaaattaaa aagtgtttcg ataattggta taatactatt tgtaggtata
     8821 ccactgccaa ctacaggaac atggactgca tctgctatag cttccatatt aaaaatgaga
     8881 ataaaagatg cttttatggg agtatttctt ggtaatttgt tatcaggggt tatagtttca
     8941 gctctttctt tacatataat ataaaactat ctaagtaata tacttggata gtttttttat
     9001 gagtatatgt gaaaaatttt taataataaa taaaaaatga gataaagtat ttaacttcag
     9061 ttagttctaa aatgtttact atggcaaaag aaatatagta tagtattact tgtagggaaa
     9121 gtactctaaa tttgtctgct aataaaatga aatttttata tagaataaaa ttttagtgat
     9181 acattttaat tattggattg aggaggtttt aaaatggaaa attcaaacac taacttaggt
     9241 agtgaaagtg tgggtaaatt attatttaaa cttgctacac ctgctattat cgcacaaatt
     9301 gtaaatgttt tatacaacat agtcgatagg atttttattg gtagaatgga aaatggagaa
     9361 gttgctatgg ctggtgttgg agtggcattt ccaataataa ttataataac tgcatgtagt
     9421 tatttaatcg gtatgggagg aggtccatta gctgctataa aaatgggtga gcaaaacaat
     9481 gatgaggcag agaaaataat gagtaacagt ttttctgtac tggtgatttt agcaatactt
     9541 ttgactatag gttttaagat tggaaaagag cctttacttt ggatgtttgg agcaagtgaa
     9601 tctacaattg gatattcaat ggactattta aatatttatt taataggtac agtgtttgtt
     9661 cagatttcaa tgggtatgaa tacttttata aatacccaag ggtttgctac aactggaatg
     9721 atgacagttg ctataggtgc tttaataaac ataatacttg acccgatttt tatatttggc
     9781 tttaatatgg gtgtaaaagg agctgcatta gctactataa ttgcacaagg cgtttctgct
     9841 atttgggtgc ttatgtttct ctttggaaag aaaagtatac ttaaaataaa aaagaagtat
     9901 atgataccaa aagcaagtat catcttacca gtgcttggct tgggaatctc tccatttata
     9961 atgcaatcta cagaaagttt agtacttata gccttaaata gtaaattaca aatgtatgga
    10021 ggagatttag cagtaggttc aatggccata atgagcagta taatgcaaat tcttatgctt
    10081 cctaatatgg gggttacaca aggagcacaa ccaataataa gttataatta tggttcaggt
    10141 caacttgata gagtaaaaaa gacatttaag ttgtgtttat tgtcatgttt tacatattct
    10201 actattttat ggcttttatt aatgatattt cctgcatttt ttgttagtat atttaataag
    10261 aatccgcagt tacttagtat gactagttgg agtataaaaa tatattttgc tggtgctttt
    10321 atgtttggga ttcaaatagc ttgtcaacag acatttttag cacttggaaa agcaacaata
    10381 tctttggttt tagctctact tagaaagatt gtacttttaa tacctttaat atttatactt
    10441 ccaacattct ttaatgaaaa actttttgca gtaattttag cagagccagt tgcagatata
    10501 acagctgcta ctataacagc aatttctttc tttatatttt ataaatgttt tctatcaaaa
    10561 cctaaagcaa taaaagaata agatatacaa tataaatttt ttatgaaaat atttt
//
LOCUS       DAENCV010000127        10570 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAENCV010000127 DAENCV010000000
VERSION     DAENCV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.129"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            134..913
                     /locus_tag="KQD82_003121"
     CDS             134..913
                     /locus_tag="KQD82_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG4381576.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1223..2044)
                     /locus_tag="KQD82_003122"
     CDS             complement(1223..2044)
                     /locus_tag="KQD82_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG4381577.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDTDVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(3054..3872)
                     /locus_tag="KQD82_003123"
     CDS             complement(3054..3872)
                     /locus_tag="KQD82_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381578.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4040..4621
                     /locus_tag="KQD82_003124"
     CDS             4040..4621
                     /locus_tag="KQD82_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381579.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCIINGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4618..4812
                     /locus_tag="KQD82_003125"
     CDS             4618..4812
                     /locus_tag="KQD82_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381580.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4976..6718)
                     /locus_tag="KQD82_003126"
     CDS             complement(4976..6718)
                     /locus_tag="KQD82_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG4381581.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKIQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            7147..8523
                     /locus_tag="KQD82_003127"
     CDS             7147..8523
                     /locus_tag="KQD82_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG4381582.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8858..9529
                     /locus_tag="KQD82_003128"
     CDS             8858..9529
                     /locus_tag="KQD82_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG4381583.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9718..10515
                     /locus_tag="KQD82_003129"
     CDS             9718..10515
                     /locus_tag="KQD82_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG4381584.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRVVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3915 a         1273 c         1602 g         3780 t
ORIGIN      
        1 taacaattat tgaaaatgat ataaatataa tatctcaaat aatatgaata taacatctga
       61 atagagggtg ttttatttgt aaaaaataag tttacagttt gagcgtataa acttatggta
      121 aaggagaata attatgagtt ataagaagat tgcaattata acgggtgcta atagtggttt
      181 tggtaaagag tttctaaagt tattaataaa tgaagaagaa ataacagaaa tatgggcagt
      241 tgcaagaaac aaggcgagat taaatcaatt agtagatgaa tttggaagta aggtaaaaat
      301 tttttcaaaa gatttatcaa aaatagaaga agtaaaagaa attggtacat ttttaagtaa
      361 agaaaatgta tgtataaaat accttataaa caatgctggt tttgcaaaat tctgttcata
      421 caatgattta agtatagatg aatctattaa tatgattgat ttaaatatta gtgctgttgt
      481 agcattggga cttatatgta ttccatatat gcaaaaggga agccatatca tgaatatttc
      541 ttcacaagca tcattccagc cattgccata tcaaaatatt tatagttcaa ctaaagcgtt
      601 tataagaaat tatacaagag cattaaatgt tgaacttaag agtaaaggga taaatgcaat
      661 tgctgtatgt ccaggttgga tgagtacaaa tctatttaag agaggtatag ttgtagctga
      721 aaaagggaca aaaaatttcc caggtatagt gtctccagac attgttgcca aaaaagcatt
      781 gaaggatgca aagaaaaata aggatatttc tgtgtatggt ataaatacaa aatccagtca
      841 tttattagca aaactattgc cacaaaaaat gatgatgaag gtttggttaa tgcaacaaca
      901 tattaaggaa taagagtttc ggtaattaag tatcagttgg agattcaatc tgttatatat
      961 tatgagaagt tttgttgagc aaataataat tgtgacaaaa cggcttatct agtgccgata
     1021 agccgtctat aatgtatata aaatatattt ttgattttac tttatttaga tattgaagta
     1081 ttaaaatatg taggacttga aattttataa agataaatac tatgatattt tgtaaaaact
     1141 gagatatgtt gatattacat agtttaacta tactgttaaa ttgaagtttt cttattttgc
     1201 attatgctta ttacttaatg atttagacta taatttccat accatcatat gcaaaataaa
     1261 tgttatcaag tttcttttct aattcactgt aataaccata tgattttccc cacatttcgt
     1321 caatatgtgt aatgataatc tttttaattc tataatagtt tttaatttca acaatctcat
     1381 ctaaagtaaa tatttcatct ttaaatggtg tgtctttaag ttttgtacca tcatttaata
     1441 taccatcatc agaaactaga cttattatta aaacatcagt atcaaagtac atatcactat
     1501 gagtaaatgg cttgggatta cagcaggcat atatgacttt tttttcattc tctttgataa
     1561 catagaaggt tatattcctg tctggattat tgttaatcaa atcaatatca aaattattta
     1621 tctttacatg tgaagtttca ttaatagaaa tgcaatttat aacatcacca taatatttaa
     1681 gacaatcgag attcgttcgg tttatatctt caattacttc aggaagcgca taaaagccaa
     1741 ttggattatt ttctcctgtg ataaagtcac atcctatttt ttcgacaatt ctaataccct
     1801 taacatggtc tgggtcatgg tgagaaattg aaatatgttt tacttccgat attttttgtc
     1861 tgttacaagc tactgcaata tcttcaggtg tgtctatcag catttttata tcatcaatat
     1921 ataaacttgg ccctaatcgt tcatatcttc cgcctttttg tctggcttct tcacatattt
     1981 tgcatttgca aaatgaactt ggaataattg acataccgcc actaccaaca atttttaatt
     2041 tcaaagttat tacaccacct tcttaaaata ttagattata taaataatat acaagcacaa
     2101 ctgattaaca aaattatgag ttaataaatt atatcacatg caaatatgaa agaatagtat
     2161 gaaattttta taaacatata ttaaatgttt ctcaatgaaa ttgactttgt atatgaactg
     2221 aatgacttat tcaatatgga taagacattt ttcttgtaat aagatatgat tgtcctataa
     2281 ggtttatgta attttgatat aatttgatat aatatattga ttaacaacat acatgttatt
     2341 ggtgagcata tttctataga atggaatata taatttttta tatttatcat tatatcattg
     2401 tctaaagaaa gttaaaaaga gtagagcaca aaggaattat ttgtacttta ctcttttatt
     2461 gttttgttaa aaaaactaat ttatattagt gattaaaata tatttaacta gagtataagg
     2521 atagtatata atcttataat tgatttatct gacaaattaa gataatatcc taatcaaatt
     2581 ctagtcattt aaacataaaa tcataattga tttatctgac aaattgagat aatatcctaa
     2641 ttaaattgta ggtatttaaa cataacatta taaataattt atctgacaaa ttaagataat
     2701 atcctaatca aattctagtc atttaaacac aacatcacaa gtgatttatc tgtcaaatta
     2761 agataatatc ctaattaaat tgtaggtatt taaacataac atcacaagtg atttatctgt
     2821 caaattaaga taatatccta attaaattct agtcattcaa acataacatc acaagtgatt
     2881 tatctgacaa attaagataa tatcctaatt aaattgtaga tatttataca taacactaaa
     2941 agtaatttgt ttaacaaatt aagataatat cctaatcaaa ttgtaggtat ttatacataa
     3001 cactaaaagt aatttgtcta acaaattaaa atattatcca attaatataa aaattacttc
     3061 tttttaactg ggaagcatac ctctgttacc caattatcag gattactatc gttgccagga
     3121 cttacatgat aaatattaaa cataggacca tctacatcat agttattatc tgatatccaa
     3181 ttaccaatag cctcacaagc gtctgcaact tgattgaaat ttccattaac aattgcagtt
     3241 gcagcagtca cacttggaac agtcttaaat ttaacatgtt cagtatcttt atactttcca
     3301 gatacagcaa cttgtacttc aacatctaca taatcttctt tatatccagt atcataaaaa
     3361 actgctttag aatactttgg gggttcaatt tgaacattct ggtcttttgt ctctaaaaat
     3421 acttgatgcc ataacattcc ttcgtcttga taagttggta taacccttct aagtgtcatc
     3481 atatattttg gtgcaaaatt ctttatagtt acatcataat ttttcataat atcatttcct
     3541 ccaattcttt taatagtggt ttctattttt aatagttttt tctgtgtatc ttctaattgt
     3601 tcttttactt gagaatggtg gatatttaga tattttatga gactttcttt atcgttatat
     3661 tctgtcaaaa tttcttttat agaatacaag ccaaagccca tatcttttag tgcatggatt
     3721 ctgtttgtag tagaaagttg gtctgcacta tagtatctat atccattagt tttattcgta
     3781 tgagaaggag ttaaaagtcc tatttcatca taatgacgta acatacgaat actaatttta
     3841 cttagctttg aaaaatcgcc tatcttaaac atgtttttgt ctcctttata aaggtacttt
     3901 tagcttaata ttaatataaa ccatatcata atgttagagt caactaaaat tataattaaa
     3961 ttatattgac atatgatgaa aaaaatatta tactgaacat gttcagaact gaaaatgtta
     4021 attgttttgg aggtctatta tgaaaagtaa tgataaagat gctagagaac gtataattga
     4081 agttacattg aatcttttaa atgaagtaga tgatattgag gagattacag ttagaaaaat
     4141 tgctgagcgt gcaaatgttg gagttggttt aataaattat cattttaaaa caaaggataa
     4201 tttgttgagt actgccatag gagatgtgat gtcaaatata atagcagagt tatatgacga
     4261 tagtgtatat actctaagac ctattgaaga tttgaaaaat ttattaaaaa aattgtgtga
     4321 tactggatta cattatgaaa aagtgcttcc ttttgtgttg aatcaatgta taatcaatgg
     4381 agatatgcaa gcagaacttg atattgtccc tatgcttcgt aaaatttttg gaaataaaaa
     4441 agatgaaatg agtctaagaa tcattgccct gcaaattatt ttaccaatac agatttctgc
     4501 tttatctaca gaatcatttc agttatacag tggtataaat ataaagaata aatatgagcg
     4561 agataaattc attgatattt taatagaaaa tattattgga gaggatgttg atgtaagatg
     4621 aaaattataa ctattggttc aagtcttatc actgttttac tttttctttc aactatggtt
     4681 tgtgggttct ggattaagaa taataaggta acagatgcaa gttctattaa gttccatatg
     4741 aatagtgcta tttttacagg gatattttta ttgatttcca caatcctttt aattatatat
     4801 ataaaaaaat aataatatta ttaataaata aagtatcaga aattataaag acaagagtct
     4861 taaaaatttc tgatacttta ccatataatt ttattaaaca ttaattgtgt gtgaaaaaat
     4921 atcacttatt tcaatctaat actaaatctt aagaatgagt ataaagtatt agaaattaag
     4981 aagcattttt tagttcaagt ggattatcta taccaaaata atcatataaa gtagagctta
     5041 tttcctctaa atttccattt tggaaaggta attgtaaatt taccttttct accacatctt
     5101 taaatccagc atcagtattt tgatttaaaa aatccatata tacatcaaca gcttttctgt
     5161 aatcttcttg tgataattgc catatctgaa gtgatacatc agatgcaaga ccataactca
     5221 agtaataaaa aggaacttgg aagttatgag atacaagtat ccaaaaagga gcattttttt
     5281 ctctaagtat attattggaa actccatatt cttcctctaa atcaaaaaac aatttattga
     5341 tatcatttag actcatatat ggattcttgt atataatttc ttgaaattca tcataaagac
     5401 aagcatcaat aatagtattt aaaacaatcg ataagtgttc tttttttata gcttcagatt
     5461 gtttaccaaa aaaatcatca aaatatttat aaaatagaat ttctaaagat gttgaatgaa
     5521 cttcacatac atctatactt ggttgtatct tattatttaa gttaatactg ttgtagtaat
     5581 tataaaaatg accaaactca tgagcaaaac tagtaacacc taaaaaagaa ttgtcccatg
     5641 tattaaacaa gaaaggtgct ttatatttat ttatataagt agtatatcct ccaggtgatt
     5701 tgttttttcc agaagaataa tcgtacaagt catatttttt catatagtcg aagctctctt
     5761 ttagttttgg agatatatct tcaagtactt tatcaaactt cctaaatgag cgatttttat
     5821 atactttaat ataaatagaa gaatcagatg gtttagatga aatctctcta tataatggaa
     5881 ctatgtaatc ttttacgtct tgtctaaatt ttttagcttc ttcttgagaa tagtctttgt
     5941 ttaggttcat ataggcataa tctatataat tcttaaatcc ttgcttactt gctatttcag
     6001 ttcttgtctg tataagttct aagaatatgt taccaaatat aggattgtat ttttttaagt
     6061 aatctgaata aattttcaca tattcttcag ggctaagatt tggtcttgac aaggcttctt
     6121 caaagtctac ctctatacca tctattgata ctgtagattt agctaataag tcttcatatt
     6181 ttacaactag gtcttgctct tttttaagca aaccttcaac ttctttagaa ttaagttttc
     6241 caatgttctt tagataatta aatgtatcct ttccaagttc tttttctaat tctttagaga
     6301 atttagaagt tacaaatcta tcttgaaagt ctttgtatga catgtcaaca tctactgatt
     6361 tactcataag atacttatat tccttagagt aaaattcgtc tgatgaatct atgttatttc
     6421 taatttttgc tatagtaagt gtactgtaaa aatcttgata aattttatca acttcattaa
     6481 ataaatttaa ttgttcctta gctgttttag ctgttaaaag tttgtcgttg cacttgttaa
     6541 tgttttcgca aattgactgt atattaggac gcttatattc cattttttca aaaggaatag
     6601 ttcctctaac agaatctttt tttgtatcat tagtataatt tcttatgtat gatgcaaata
     6661 taataaaaac tgcaattaaa attagaaaaa caaatatttt cttcttggat ttaaccataa
     6721 aaatcctcca atcatggata tagttattaa ttattttact atattaaata taaaaaatgc
     6781 atgttaagaa aatttaacat aaagatatag ataagattat aaataatata aaaaatttaa
     6841 cttcaaaaaa gtatatgaaa tttatactgg taaaatgata aataaatttt aagtacaaaa
     6901 tagtaaaagc tatgatagtt ttagacctat tatatgtaaa aaatatagat gtttttggtg
     6961 caatttaaat taaaagatat atataaaaat ttaaaatatt ccaaacagaa attataaaaa
     7021 ttactatcat tgtctttata caatatttat actataatag aataataatt tagaggaaag
     7081 ctctaaaaat aaacaaatta agggtcttaa cagtagtgat tttatgttaa ggaggtataa
     7141 agaaagttga aaacaatagt tagaagactt attctctacg cagatggaat gcgtcctact
     7201 caaataatgg tatctgggtt tgctgcgatt attgtaatcg gagcattgtt gttaacactc
     7261 ccgatagcgt cgcagagtgg agagagtata ggcttattaa atgctctatt tacggctact
     7321 tcagcggttt gtgttactgg tctagttatg gtagatacag caacttattg gagtttattt
     7381 ggacagatag ttataataac attgatacaa attggtggtc ttggatttat gaccgtagcc
     7441 acaatgtttt cacttatggc acgaaaaaaa atacaactaa gagaaagatt attaatacaa
     7501 gagtcattaa atcaagctga tttatcggga cttgtaagac ttacaagatt tgtattaata
     7561 ataaccataa caatagaggg aataggtgca ctggttttat caactgtctt tattccacaa
     7621 tttggtttgt ctaaagggat atggtatagt gtatttcacg caatatcagc gttttgtaat
     7681 gccggatttg acttgatggg cagtgtaagt ggcccattta catcgttaaa ttcttatgta
     7741 aacaacttta cagtatcaat gactgtttgc gcacttatag tgcttggagg gctaggattc
     7801 cctgttgtac ttgatattgt aagaaaaaga agattttcaa aattaaatgt gcattctaag
     7861 gttgtattgt tttcaactgc tacacttatt tttgtgggtg cattatttat ttttcttata
     7921 gaatttaatc aaaaagctac tatggcggac ttgcctttaa agggaaaagt actatcagca
     7981 atatttcaat cagtaacagc aagaactgct ggttttaata cgcttgattt agctacttta
     8041 cgagaaagta gtgtatttgt aatgataatt ttgatgttta ttggagcatc acctgcatca
     8101 actggtgggg gtataaaaac aacaacacta gctgttttaa tcattacagt tagaagtttt
     8161 ctatcaggaa aatcagatat agaagctttt gaaagaagat tagctccatc aactataaaa
     8221 aaatctcttg gtatatttgt aatcagtata tcagcagtta tttttggaac attgattatt
     8281 tcaataactc agcctaattt tacattagtt caatcagctt ttgaggttac atcagcactt
     8341 gctacagttg gttcaagttt agctggaact ccaaacttaa atgctttagg taagattata
     8401 ataataatct ttatgtttat gggtagagtt ggttctctta ctttatttat ggctatactt
     8461 tctggtggta gaagaaagag tcaaccaatc agatatgcag aaggtaaaat tatggttggt
     8521 taagttacca agaagtagta taaatttata atggatttag agtagcactg taaatctgtt
     8581 gataaaataa atgagcctag atgtaattat atgaagaaat aaaatcgact aatgaacaaa
     8641 attaattaat gaaataaaaa aacagggaaa taaaatacaa ataaaaaggt aaatcaaaat
     8701 taaattaatc tgtgtatata aaattatata ttgtgtataa gttttgtatg tgtaaagtat
     8761 ataagttttg tatgtgtaaa gtatataagt tttgtatgtg taaacaatta atttaagaga
     8821 tattatataa attgtgtatt agggaggaag tttcgagatg aagcaatata tagtcattgg
     8881 gtgtgggaga tttggaagtt cagttgcgtc tactatgcat cttttaggac atcaagtaat
     8941 ggcaatagac aaaaatgaag attcagttca aagtatatct gacaaggtaa cccattcact
     9001 tatagtggat gttactgatg agcaagcgtt aaggtcatta ggtttaggta actttgatgt
     9061 agcagtagtt gcaataggtt ctgatataag ggcatctata atggcgactc ttatagccaa
     9121 agaaatgggt gtagagttga taatatgtaa ggcaaaggat gaattacaag ctaaagttct
     9181 ttataaaatt ggtgcagata gagttgtatt tccagaaaga gatatgggag taagagttgc
     9241 acacaattta gtttcggata atatattaga ccatattgaa cttgacccag agtattcaat
     9301 tgttgaaatc gtaactccaa atagttgggt tggcaagaca cttatagagc ttgaattaag
     9361 agctagatat gagataactg tacttgctat aaaaacaggt aaaaatataa atgttacacc
     9421 ttctccagat gaggaactta cagccggaag tatcctagtt ataatcggtc aaaatactag
     9481 tataacagcg ataacatctg gaaataaggg gataattaga agaagataat ttactattta
     9541 atatatattt aattgcaatg aaagtagata gtatcatata attatgaata gttatatgat
     9601 actatttttt taatcgaagg tagtatttat ttgtaagatt agataaagag aaattaaatt
     9661 aaaagtaagg aggctgtgct aatataaaaa tttataagtt attagcattt agataaaatg
     9721 attacaaata taaacagtaa ggataatgaa aagttaaagt atacaagagc actattaaaa
     9781 tcaaagaata ggaataaaga gtcaaagttc ataatagaag gatacagagt agtaatgctt
     9841 gcacttgaat gtatggcaaa ccttgattat gtatttatca atgaagaatt tgaaaataag
     9901 aaagaacatg taaaactatt agaagatttg gataaaaaaa acacaaagat atacaagact
     9961 actaataaaa actttaaaga attagtggat acagaaaata ctcaaggaat aataggtgta
    10021 gtttcattta agaaaaaaaa attaagtgaa agtataaata aaaaagataa atttgtattg
    10081 attttagaca gaatacaaga cccaggaaat atggggacta taataaggac tgctgattct
    10141 gctggagtag atgccataat agcactaaag ggatgtgtcg atatatacaa cccaaaagta
    10201 attaggtcta ctatgggttc tatttttgat atgaatataa ttgatgcttc acaagatgaa
    10261 actgtggaca tgcttaaatc attggatttt aatatagttt caagttactt aaatacagaa
    10321 aatttttatg acaaaataga ttatggctca aaagtagcat tggtgatagg aaacgaagca
    10381 aatggaataa atgaagaact tgtatcaaag tctgatattt tggttaagat acctatatat
    10441 ggtaaagccg agtcgttaaa tgctgcgata agctctgcta tactgatgta tgaaataaaa
    10501 aaatacttaa tttaatgtat tgtaataaat ataatgtcat gttataatct tgaattaaat
    10561 agtagagtat
//
LOCUS       DAENCV010000128        10569 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAENCV010000128 DAENCV010000000
VERSION     DAENCV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.130"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(111..680)
                     /locus_tag="KQD82_003130"
     CDS             complement(111..680)
                     /locus_tag="KQD82_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG4381585.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            883..1110
                     /locus_tag="KQD82_003131"
     CDS             883..1110
                     /locus_tag="KQD82_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG4381586.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1329..2072)
                     /locus_tag="KQD82_003132"
     CDS             complement(1329..2072)
                     /locus_tag="KQD82_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG4381587.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAAPQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2240..3112
                     /locus_tag="KQD82_003133"
     CDS             2240..3112
                     /locus_tag="KQD82_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4381588.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3112..3777
                     /locus_tag="KQD82_003134"
     CDS             3112..3777
                     /locus_tag="KQD82_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG4381589.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3798..4655
                     /locus_tag="KQD82_003135"
     CDS             3798..4655
                     /locus_tag="KQD82_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4381590.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEKGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4811..7357)
                     /locus_tag="KQD82_003136"
     CDS             complement(4811..7357)
                     /locus_tag="KQD82_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4381591.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7357..8043)
                     /locus_tag="KQD82_003137"
     CDS             complement(7357..8043)
                     /locus_tag="KQD82_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381592.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8126..9115)
                     /locus_tag="KQD82_003138"
     CDS             complement(8126..9115)
                     /locus_tag="KQD82_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381593.1"
                     /translation="MFRNREFRKLAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9102..9788)
                     /locus_tag="KQD82_003139"
     CDS             complement(9102..9788)
                     /locus_tag="KQD82_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381594.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10288..>10569)
                     /locus_tag="KQD82_003140"
     CDS             complement(10288..>10569)
                     /locus_tag="KQD82_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381595.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3718 a         1747 c         1545 g         3559 t
ORIGIN      
        1 ctataaaagc taaaaagttt ttctattcgc tacatctaaa aataattaga tagaggtata
       61 aatgtattta gtttatttat acctctagtc ttttataaat tatatctcta ctaaattgat
      121 tttttagcac gaataagtgt gctcttggag ataccagtca tcttcacaac ctctctatat
      181 ggcttagaat ttaataaccc caatgcatat tgtatctgtt gattgctgta tttctttggc
      241 ctaccatctt taaagtcctt tttcttttta gcaaattctt ttcctaattg agtacgttct
      301 attatcatat ttctctcaag ttctgcaact gcaagtaatg tagtcaaaaa gaatttaccc
      361 attgaagtat tttctaaaag gcctatattt aaaatatgaa cacttacttc tttagcaaat
      421 aaaccttcta ttagttgtat tccttctgat gtacttctag ctagtctatc cagcttagtt
      481 gcaacaagta agtctccctt ttttagagat gatataattt tttcaaattt aggcctgttt
      541 gtagtagtgc ctgtataagt ttcctttatt attttagcat cattatattt attcaaaatt
      601 tcttgctgtt gctgatacaa actgttgttg tctgtttgat tttttgtact tacacggcaa
      661 tatccatata taataatcat cacttcctaa cataaagatt ttgtacagaa gttacccaga
      721 agcttgtttt tacttatctt tatccatttt actaaaacga tttttgggtg tcaatactgt
      781 taacttttga caccataata attcctttat aatttgtttg actatgtcaa aaattaccaa
      841 tataatgaaa ttaatgagtt tatacaaatt ggaggaaata atatgaatag tctgttaaaa
      901 gaagcaatag atgaattaaa aaacatccat tgcggggaag tttttttatt aaaagaattg
      961 ttcaaaggct atgaatggaa caggttggag atgaatatca gattgaaatt aggaaaactt
     1021 tttttgtatg aagtggtaac taatcccaat ttaaatgtag aggtcatcaa taaaaattct
     1081 gcttatcagc aaatgtataa aaaaaaatag tgacttaaat gtatataata gtataatctt
     1141 cttattgatt caaaagttaa aacttttaat acaaaacaat cattctattt taacatttat
     1201 caatatgtct aaatttatat aattaacaca tacgaatagt attgtttaaa tcacattatt
     1261 taagatagat aaatcttaac cctactccag cataaatact aaaatagggc ttagaattat
     1321 ctatattttt atcccataaa atgcttagct cttcctcttg attgaacata tatatctggg
     1381 gaaatacaat agaagcgtaa atttataaaa tttccaaagt cagaaaactt cagttcaaac
     1441 ttaggtatac caaaactttc aggtgctatt tcgtcaacac caaaatagat tataattcca
     1501 ttatctgtaa gataaaatgc ttggtcttca ggtataacta actcaacatt agaataatac
     1561 atttctctat tttcatttat ttgagattta atgtaatcag taattatatt aatataatca
     1621 actccatctc taaaaatgtc attaagtaaa agttcttttc cagttgtcaa atctatattg
     1681 taattattca atgtattgta tttaggccca tagttattag caaaccccat aaggtttaat
     1741 atcatactca gtacataatt tttattaaaa gtaactgcgt aactggtaga tacccaatag
     1801 ctttgttttg gaagtgaatt ttgaggagct gcgttattat attctcgttc ttcttcctgt
     1861 aagccatttt taaaattatt tacagtcatt cttacagcat tgtttatgtt atttaaaata
     1921 actgggttta catacgtgga ttgatttcta atattgtata taacaggata ctttacatca
     1981 tatgaaaaat acttacttct ttcaaaatct tcattaaatt gtacatataa tatattatat
     2041 gtacaggtat ttgtagattg ataattttcc attaaaatcc tccccctttt ataaaaaaat
     2101 cagatacaat ataatatgct taaaacagta ataatgttaa tggtaaatgt aaagaataga
     2161 aagtatctaa aacagcaaaa aatgactaat tttgtggtat aatacatagg attgagagtt
     2221 attttatagg aggaaaaata tgttattatt agcagataaa tggaaagatt atgaacttat
     2281 agatatggga aatggagaaa aattagaacg ttggggaagt tatgtactta gaagaccaga
     2341 cccacaggtt gtatggccaa tggaatctga atgggcttta tggaaaaacc ctcatggaca
     2401 ttatcacaga agcaataaag gtggaggtca atgggaacac aaaaaaagat atccagaaca
     2461 atggactata aattataaaa acttaaagtt tcacattaaa ccaactggct ttaaacatac
     2521 tggattattc ccagagcaag cagctaactg ggattggatg atagagaaaa taaagaaagc
     2581 taacagacct ataaaagtgt tgaatctttt tgcatataca ggtggagcta ctgtagcatg
     2641 tgcatctgct ggtgcagaag tatgtcatgt tgatgctgca aaaggtatgg ttaattgggc
     2701 aaaagaaaat atagaattat caggtcttaa aaatcaaaca gtaagattta tagtggatga
     2761 tgttgtaaag tttgttgaaa gagaaataag aagaggtaaa aaatatgatg caataataat
     2821 ggaccctcct tcttatggaa gaggtcctaa aggagaagta tggcaaatag aagaaaagtt
     2881 atacagtttt gtagatttat gtatgggagt tttatcagat aaccctttat ttttcttaat
     2941 taattcttat acaacaggat tttctccaat agttcttgaa aatgtattgt ctacaacaat
     3001 aggtaaaaag acaaatacag gagatatata cggtggggaa ttaggaatac cagcttccag
     3061 agatggtaag atacttcctt gtggtatatt tggaagatgg gagaaaaaat aatgataaaa
     3121 gtcatatatg aagataacca tttattagtt gtagaaaaac cagttaacat attgtctcaa
     3181 ggagataata ctaatgataa agatatggta aatcttttaa aagactatat taaaaaaaag
     3241 tataacaaac ctggaaatgt atatattggt ttaatacata gattggatag accagtaggt
     3301 ggagttatgg tgtttgcaaa aacatcaaaa gcagcatcta gattgtctga gcaggtaaga
     3361 agtaagactt tcaaaaaaac ttatctagct gttgttcatg gcaagataaa atctaactca
     3421 gatatactaa aagatttttt atataaaaat aaaaaaacta atatggtaac tgtagtgaaa
     3481 aaaagccata aagatgccaa agaagctgaa cttaactata atgtattaga ttttaaagaa
     3541 aatctaagtt tagttcaagt taatttaaag actggtcgtt ctcatcaaat aagagttcaa
     3601 tttgcaagca gaaaacatcc attatacgga gaccaaagat atggcaaaga aataaataag
     3661 attggacaac aaatatctct ttggtccaat aagatagaaa tttgccatcc tactacaaaa
     3721 gaaaaaatgg aatttacatg tcatccacct gacaaatatc catggaatat attttaaatc
     3781 tcaaatgagg tgattatatg agtaaaaaat tttcaatagg agaaatgtct aaacttcata
     3841 aaataagcat acagacacta agatactatg accaaataga tttatttaaa cctgttcatg
     3901 ttgaccaaga aagcaattac agatattact caattgacca attttcacaa ttagatatta
     3961 taaaatattt aaaatattta ggtatgcctt taaaagaaat taagtcaaag cttgatgaaa
     4021 ataatgaaga aattctaaaa ttactaacaa ataaaattaa tatagtggat gaaaaaatta
     4081 aagaattaga gcttataaag aaggtactaa gcacaaagaa agaaactatg gaaaaaggta
     4141 tgaaagtaga tgaagttggt aaaatagtga gaaaacatat tccacgaaga agtatcttat
     4201 ctatagagta tgaaaaagga ttaagtagtg aagaaactat ggaactttcc agaagacaaa
     4261 tagcaaatat attagaagat aatatatctg tgttctatgg tggagtaagt ggacttgtat
     4321 ctatagataa atttttaaaa gaagataaag ttatttatga caatagtttt gttactgttg
     4381 aaagagactt atttgatata aaatcacaaa aacatataac agaaatttca tctggtgatt
     4441 ttatttgtat gacatataaa gggccttaca aagaaaatta taccgctttt aagaaactgg
     4501 ttgattatgt tgaatataaa agaataccta ttgaaaatta tatatatgaa atacctataa
     4561 tagaccctct gtctacaaac aacgaagagg aacttctaac agaaattcaa atacaagtta
     4621 aaaagagaga aattgactta agatgtctag tgtagtaatc tagtttactt acacatctta
     4681 atatagttgt ctattatcaa catatgattt ttctagatag tgattcctaa cagtgagatt
     4741 ttcaaaaaat aggaggaaat atggctaatg ctaattccct cctattttta attaatccat
     4801 atcattttaa ttaagccatg tcatcacgaa gtgcatcaat aatattttct ttttttatct
     4861 tattaatagt atacaacatt gtaataaata ctataaataa aacactaaat atactaattc
     4921 caatactaaa ccatggaatc acaaaatcaa tatcatctgt aaccattact ttatgaatta
     4981 gccatgaaga aattactgct ataggaagtc caaagaacaa tgctctcatg ccataaaagg
     5041 cacactcaaa attcatcatc ttttggaaat catgctcaga cattcctaca gagcgaagca
     5101 tggcaagttc tctacgacgt aacttaatat tagttgaaat tgtattaaac acattggcaa
     5161 tcgaaatcag agaaatcata attatgaaag tataggcaaa aacattggca ataaaaatgt
     5221 aattacggtt ctcatccatc attccataca tattgtacag attgtactca ttcgtaactc
     5281 cctcactctc aataatttgc tccatttctg caactgactg agtaggattt ttcgattgaa
     5341 agcttaaact ttcaattcct gtagaaacac caggaatctc aaacttatct ttggctgaat
     5401 aaggagccaa tactctgaag cttgctttct tcacatctga acttctgatt cctggaggag
     5461 aatcaggtgg cactactttg acaaatgaaa tattcacatt ttgtctctca ggtatctcca
     5521 acttgccatt agtttcagga gtgaggctaa cattcataga agaactgttg aaaatatcaa
     5581 taatattatt atcctttgtt accatagaaa cagcaatcag ttttgcattt tgtccagtat
     5641 attcttctgc tggtaatcca agacttttaa taatattcag ataagttcta tcatcaagaa
     5701 attgcatatc tactggtaat tcaagcattt cactcataga atgtgaaccc tcatagttca
     5761 aataatcgtc tgtaagctca cttactttgg caacacatga ataggtcata agtttctgat
     5821 acaaaccttc gtaaacacca tttgcagttt ttagcttatc gtaaagcttc aacatttcgt
     5881 tgtctttcat accttgtgta gaaaaaataa tatcctggtc agtaacctct gacctctgat
     5941 ttgacatctg ttttaggtct gctactaaag cacttgtaga tataaataga actatgctta
     6001 aaacaagcga caatacaata cttcgataac gtttcttgtt tcttttaaag tttttcatag
     6061 caagaactcc ctcaagacca taaactcgct gtgttagttt tgatgtcttc acagatttag
     6121 attcaatttt tacctcattt gtctgacgaa tacactccat cactggtgtg ttggcagcct
     6181 ttcttgctgg tatgtaagca gaaattaaga ttgtaaccat acttaccact gctgcaacga
     6241 tgattgcagg gatagacaat gttaatgtca acggaacgtt tacatagaga atatttgcaa
     6301 agtttttagc tatgacagaa atcacaagtc caatactgac tataccgatt gcaacaccaa
     6361 ttggtatacc aatcgcacca atgcaaattc cttcaaatag caccgaattt cgtaactgtt
     6421 ttgctgtagc tccaacagat gaaagtattc caaattgatg tgttcgctcg ttcaatgaca
     6481 tgttaaatga attatatatt agaaaaattg agcctagcat gattatggca agcacaatgc
     6541 caccaaccga gtacaaaaac atattgaaca aattgttatc agaaacacct atgaagcgca
     6601 acacattgtc attgaaaatg gaagcatggt ctttagtgct acttatgtaa gagcgaatct
     6661 gatgtggatt tttcaatgtg acaaaaaggc taagactgtc agtcttgact tgtttatctt
     6721 ctttggttat caaggtatat cctggtgcag aagattcttc aaatacgggc tttctacaga
     6781 tacccacaac tgtataagtt ttcttatcct gtggcacaaa aacttcactc ccagcagtgt
     6841 atgggtcact ctgaccaagc ttttcatttt cactcattcg tttccctaca tccaaagaaa
     6901 tagtatcacc tactgcaaat ttgacaccac catctgtcgc aactttactt gaaacaagaa
     6961 tctctctact attttcaggt aatcttccag aaattaaagt tataggcaaa gtatcaaaag
     7021 cttctttgct aaatccagca ataaaaagat atggtttatc tggatttctg ttaccttcaa
     7081 gtgttgcata gccaatattt tcaaatgttg cagtatttgc aacttcatca ttgactcttt
     7141 gttcctgtac aaaagaagaa tccacatcca aaaactggac atgccaatca ccatatttta
     7201 taatagctcc atttgtcata tagttcatca aagaaactcc aaaggtagta actgctgtaa
     7261 tcatagcagc tgacagaaca actcctataa ttgttacaag agttcgagtg cgattctttt
     7321 tcaatccttg cagagcaact ttgttaaaaa tgttcattaa cgattcacct tcctctcatc
     7381 acgcactact tttccatctg aaatacagat aattctatct gcttgtaaag caatgttttc
     7441 atcatgtgtg acaataatga gagtttggtg atatttacta tggctctctt tcagtaactg
     7501 gataatctct tgtccatttt tactgtctaa gctacctgta ggctcatcag caagcataac
     7561 tgctggtgca ttcatcaatg cacgaccaat ggcaacacgt tgctgttggc caccagaaag
     7621 ttgatttggt aaatgtgttt ttcgttcttt taaaccaagt aattctaaca agtcatttaa
     7681 ccgttcttga ttgaccttac gtttatccat aagtataggc aatgtgatat tttccactac
     7741 atttaatgtg ggtatcagat tgtgaaactg ataaatcaat ccaacttgtc gcctacgaaa
     7801 aatagcaagt ttttcattgc tttggccata tacatcttgc ccctccagat atatctttcc
     7861 acttgttggt acgtctactc ctgcaatgga atgtagtagt gtagatttac cagaaccaga
     7921 agaaccaatg attgcagtaa actccccttt ttcaatagta agtgagacat ggtcaagtgc
     7981 agtaacctta ttttcattct taccataaac cttacataaa tcttcaatct ttaaaaacgc
     8041 cattatataa gtctcctttc atattttata aatatatcat aggactttca acttacattt
     8101 aagtgacatt taaatgagag attcgtcact ttggaaaacg aataacaaat attgcaccac
     8161 cttgtgggtg attttgtgca gtaattgttc ctccttgtct cataataatc gtcttacaaa
     8221 gtgccaatcc tattccataa ccagctgtgc ttgaattttt cccacgataa aatctgttaa
     8281 atagacatgg taagtcttct tttttaaagc ctgctccgtt gtcatgaata gtaagttgag
     8341 taaacaaaaa gttgtcctca caaatgatat caatttttcc attatcaccc acactctcca
     8401 tgcagttttt aaatatattt tgaattgctt ctgaaagcca atttaaatca cccaaaatcc
     8461 ttactccttt tggtatatca atatgtaaag tgatattgtg cagttccata ggaatcaaaa
     8521 gttgatgaag tgcagaacta atcaaattat tcacatcaat ttgttctttt tgaaagacta
     8581 caatacctgc atctaaacgt gataatttta aaagggaagt aatcagccag tccattcgta
     8641 taagtaattc ctctgtttct cgtatcaact cttttctctc atcttcatca gaagtattct
     8701 ctaacagtga caaaataaga gttactgatg taagaggagt gcgaagctgg tgggctatgt
     8761 cagctagtga gtcagcaagg tgttcttttt cttttttcag tgcgtaattt tgctctctaa
     8821 tacgtaacgt cattttagtt atttcacttt gtagaataga aagctcaccc tcatctgatt
     8881 cagcaatata cagatggtta gcattatgaa gcacaagatt aatttgttct gaaatctgtg
     8941 caatactttt gtatcgattt ttagtaaaca caaaaaatgc tattccaaag gtagtggcag
     9001 aaacaataga aagtattcca gtcataatac tgattgcaaa tcccagggct acagtgagaa
     9061 tagtaattaa taagaataaa atagcaagtt ttcgaaactc tctatttcga aacataactt
     9121 ccccccaatc tataccctgt cccacgaaca gtcaaaatta tttgtggact tgaagggtca
     9181 tcttctatct tctctcgcaa gcgtttgatg tacacagtca atgtgttgtc attaacaaac
     9241 tcacctgccg catcccacaa ttcatcaagt aatcttaccc tcgtaataat acttttagga
     9301 ttgctaataa acaccaataa caaacggtat tctaaagctg aaagaaaaac ctcattgcca
     9361 ttctttttca caacaccggt tgctgtatct acataaagcc cacaaatttc aaaagttgac
     9421 ccaaaacgcc cactttttcg tagtgctgtt ctaattcgcg caatcagttc acgtggacga
     9481 aaaggtttag taatataatc atctgcaccc atattcagtc cagtaacaac acttgattca
     9541 tcaccggaag ctgtcaaaaa gataacagga atgtcttgct tttctttgat ttctgtgcaa
     9601 atcgtaaacc catttccatc aggtaaagaa atatcaataa gtgccaaatc aaatttattc
     9661 ttagcaatct ctacaagtgc ttcacttctt gtggatacat gggttactat aaatccttct
     9721 gaattaagca aaagaataag atttttggca attgctttat catcctcaac taaaaatatc
     9781 cgtttcattt actttcatcc tcctttattt tattagtttt atatattcca gcagagcaat
     9841 agtacactac tattttactt atcattacac aattttcagt agtttctttt gttcttatat
     9901 aatccatgca taatacctcc attgatttat tatatttctt ataacataaa tacacaagat
     9961 aagacaaaaa acataatgta agtgcaagtt ttttaactca ctaacttata aacatacact
    10021 cctactaaca ctactttctt gatgacacat cttacaagct tatattcata catcaatgaa
    10081 aaattaaatt tatccattta ttaaaataaa aggcaatagc ttctatagct aatgcccttt
    10141 atcttaaaat gatttaaata ctatacaatt tttataaatg gctataaata aactcatata
    10201 gaatataaaa tttatctaca gtcttttgtt atacaaaatt tataacctct tatagaacaa
    10261 atttaccact ttgcaaactt atcactttta ctcagctata ttataaactt ttaatttatc
    10321 tttgtttaaa tgtttcttct taacttctac taaagctttt agcgtatcca gagatgtcat
    10381 tatttcagta gaaaattcaa tagctgttct tctcatttta aagccatctc ttgtagagtc
    10441 atttccttta gttggtgtat ttattactat atcaacttgt ttatttctta tagcatctaa
    10501 tatgtttggt ctaggttctt caattctatt tactatatca gcctctattc cattttctct
    10561 taaactctt
//
LOCUS       DAENCV010000129        10513 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAENCV010000129 DAENCV010000000
VERSION     DAENCV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.131"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            149..3046
                     /locus_tag="KQD82_003141"
     CDS             149..3046
                     /locus_tag="KQD82_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4381596.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTEADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3367..3579
                     /locus_tag="KQD82_003142"
     CDS             3367..3579
                     /locus_tag="KQD82_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG4381597.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3592..3813
                     /locus_tag="KQD82_003143"
     CDS             3592..3813
                     /locus_tag="KQD82_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG4381598.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3913..6069
                     /gene="feoB"
                     /locus_tag="KQD82_003144"
     CDS             3913..6069
                     /gene="feoB"
                     /locus_tag="KQD82_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG4381599.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6095..6247
                     /locus_tag="KQD82_003145"
     CDS             6095..6247
                     /locus_tag="KQD82_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG4381600.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6427..7137
                     /locus_tag="KQD82_003146"
     CDS             6427..7137
                     /locus_tag="KQD82_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG4381601.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7222..7992
                     /locus_tag="KQD82_003147"
     CDS             7222..7992
                     /locus_tag="KQD82_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381602.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            7985..8716
                     /locus_tag="KQD82_003148"
     CDS             7985..8716
                     /locus_tag="KQD82_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381603.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8750..9709
                     /locus_tag="KQD82_003149"
     CDS             8750..9709
                     /locus_tag="KQD82_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4381604.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9807..10403)
                     /locus_tag="KQD82_003150"
     CDS             complement(9807..10403)
                     /locus_tag="KQD82_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG4381605.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4051 a         1096 c         1893 g         3473 t
ORIGIN      
        1 taaatagata ataataaatt cattatagtt gaataagaat attatatgag aatattgcaa
       61 tttgatatat ttaccattac aataaaagta gttacaacta gaaaacgaaa taagttaaag
      121 taagttggca aattggaggt ttttaaatat gaaagcattt aaaaagcact tcttgatact
      181 ttctttgata tttttactta ttatatcatg ttttaacaat atactatgct ttgctgatga
      241 gtctgaaaat tcaaaaaaaa taagagttgg atattgtgat gactacggaa taatatcctc
      301 atcaaaagag cattcttata caggatatgg ttatgattat ctaagagaaa tctcaaaata
      361 tacaagatgg gaatatgaat ttgtaaaagg gtcttgggaa gaatgtttag acagactaga
      421 aaagggtgaa atagacttac tcggaccttt acagaagaat gatgaaagaa ataaactatt
      481 taattttcct aaactaagtt caggatatga atatagtgca ctatatacaa aggatagtaa
      541 caataatatg ttttatgaag atataagttc ctttaaggga atgagagtag ctgtacttag
      601 aggaaacttt cataatactg catttgagaa gtatagagaa gaaaataatt tttcagttga
      661 atatatatac tgtaattcta ttgatgaatt aattgagtcc gtcaatgaaa aaaaggcaga
      721 tgcatttgtt tgtggaagta ttataaatgc taaaggaatg aaaatagtta gcaaattttc
      781 agtagaacct ttttattttg ctactgcaaa agataaacct aatttggtaa aagaattaga
      841 ttatgcttta aaagaattaa aaataaatga tatgtattat gagttggaat tatataaaaa
      901 atatttcaaa agagaggtca ataattccat agcatttact cggcaagaaa tgaattttat
      961 taaagcaaac ccaaaactta ctatggtata tgattcagag tggtctcctg tagagtatta
     1021 tgataaagaa agcaattcat ttaaaggtat aagttccgat ttaatgtcaa ttatatcaaa
     1081 aaaatgtggt ataaaatttg aatatattaa aacaaaaaat tataatgaga gtcttgatta
     1141 cataaaaagt ggtaaggcaa ctatgctatg tggctcaatc aatgaaaatg ataaggcaaa
     1201 acgatttaat atgaaattga caaatccata cataaatatt ccaatgatta tggtaggaaa
     1261 attggatacc aatctcaata atgatttaaa tattgcttta actagtagtt ataagtctat
     1321 tgattcttat atagaaaaga gttttgaaaa tgctaagaca acatcttata agagtgtaga
     1381 aagttgtcta aacgctataa atgaaggtaa agcaaactta atgatactta gttcttatca
     1441 gtttgatgaa cttttaagag aaggaaagag tgagaattta tctgttatat ctgttttaga
     1501 tacaagctat gggatgcgta taggggtatc aaataaaact gaccctatat tagtgtctat
     1561 tttaaataaa tcaatagaca aaattactga agaagaatta agtgattgta tatattctaa
     1621 tactattgac aaaccttata aagtaccatt tggtgtgatt tttaaagaat attcaataca
     1681 aattatttct tttgtatgta ttttattctt aattgctatt aaatatataa tttataataa
     1741 aaagaagaaa gaggattatc taaggaagat agcatataca gacccattga cagaagcaga
     1801 tagtatagat aaatttaaaa taaattctaa taaactgttt gctaagaata atccagaaga
     1861 atatgcgttg ttttatatag atgttgacaa atttaaatat ataaatgata tgtttggata
     1921 tgatatggga aatgatactt tgatacatat atcaaatact attgcaagtg aattaaagga
     1981 agatgaaatt tttgcaagag tttctgctga ccattttgtt cttcttatta agtataagac
     2041 agatgatgat attaaaacaa ggttaaacaa tatttacaat aaggttcaaa tacttagtaa
     2101 tcctaaaata aactattata aattgatttt agattgtggt atatataaaa taagtaaaag
     2161 tgataatgac ataaatacta ttatggatag agctaatact gcaagaaaaa ctataaaagg
     2221 tggtcataaa aattcatttg ctttttacga taaggaaatg cacaaaaaaa tattaaaaga
     2281 gaaagaaatt gaaaattcaa tggtagatgc tcttaataat ggagaattta ttgtttattt
     2341 tcaacctaaa tatagccttt cagattatca aattataggg gcagaggctc ttgttcgttg
     2401 ggataatcca caaaaaggtc taataccacc aatagaattt attcctgtat ttgagaggaa
     2461 tggttttatt gtaaatattg atttctatgt atttgaggaa gtgtgtaaaa agattagaga
     2521 atggatggat gaaggacaaa aagtagtacc tatatctgta aacttgtctc gaatgcattt
     2581 tgtaaatagt aattttatag aggagtttaa attaattgta gataaataca aaattccgac
     2641 aagacttata gaattagagc taacagaaac agcagtatta gataatatag aaggattact
     2701 tgatacaatg aacaatttga aagaaaaggg atttgtaatc tctatggacg attttggtac
     2761 aggatattcc tctcttaatc ttttaaaaga attgcctgtt gacatattaa aactagatag
     2821 agcatttttt acagaaaaag atgagagtaa taatgaaaaa attgttatat ctaatgtcat
     2881 taaaatggca aaggaactga aaatgaaagt aatatcagaa ggtgtagaga ctatttcgca
     2941 agtagaattt ctaaagcaaa ttgggtgtga tatggtacaa ggatatttgt tttctaagcc
     3001 aatgcctgta aaagagtttg aaaaaatcgc ttttaaaaaa gaataaagca atgtgtaaat
     3061 aatgacatat ataataaagg tgctttaata ttagttgagt atactatttt agagcatctt
     3121 tatttttgta ttaattttta cgattttatt aataaaataa atcaaatgag aaaaaaaatc
     3181 aaaagaatac aataatcact tgcaaaaaat atactattgt gatagactta aatcaaaatt
     3241 aggaattgta aaaagaagga aaaaatagga tgttatttta atagattaag atttaaggta
     3301 ttgtaattta tatataagat aacgattgct tgtagattta aattacttta agaggaggga
     3361 tattatatga tgccattgat attatcaaat acaggtgaag aagtagttat aaagaaaatt
     3421 tcgggaagtg ataagacgag gactttttta aatagtatag gttttgtagt gggtacaaat
     3481 gttaaaatag tttctaagat agatggtaac ttaataatag acgtaaaaga tactagagtt
     3541 gcattagata aaaaaatggc aagtagaata ataatatagg gaggtaatga catgaacaga
     3601 ctgaaggata ttaagtgtgg agagactgta aaagtcaaaa agttagaggg agaaggagct
     3661 acacgaagac gtataatgga tatggggatt acaagaggtg ttaatatatt tataagaaaa
     3721 gtagctcctc ttggtgaccc aattgaggta acagtcagag attatgaact atcaattcgt
     3781 aagtcagatg cagaaaaaat tattgtggaa taataaagat taattagagt agggttaatg
     3841 taattatata atgagaatga ttttctgtat cacaaacagt gatgtattta aataataatg
     3901 gagggaatgg aaatgtcaat aaaaataggt ctcataggaa atcctaactg tggtaagaca
     3961 acaatgttca atgggcttac aggatcatca caatatgttg gtaactggcc aggagtaaca
     4021 gttgaaaaaa aaggtggaaa attaaaagga aataaagatg tggaaatagt agatttacca
     4081 ggtatttatt ctttatctcc atatacatta gaagaagtag ttacacgtaa ttttatgcta
     4141 gatgataagc cggatgcagt aataaatatt gtagatgcat caaatataga gagaaattta
     4201 tatcttacaa ctcaagtttt agagctaggt ataccgaccg ttatagctct taatatgatg
     4261 gatattgtaa ataagaatgg tgataagata aatattaaag aactatctga agttataggt
     4321 tgcccagtgg tagaagtaac agcagttaaa ggtcaaggaa ttatggaggc tgctgaaaag
     4381 gcagttgaac ttgcaagtag taacaataaa ttaaatttca aattaccctt tgttgatgaa
     4441 agtaaggatg caatagaaaa aattgaaaaa ataatagaag aaaagactcc atatatagat
     4501 gtagaaacac gctggttagc tataaagcta tttgagagag atgaaaatgt tatacaaaaa
     4561 cttgatattt ctaaaacaat attgaatagt attgaagaaa taactagaaa ttgtgaagat
     4621 gaattggatg atgatagtga aagtataata acagccaatc gttatgaatt tattagttca
     4681 ataatttcaa gtataataaa gaagaatcga aaaggtaagg aaactgtatc agataagata
     4741 gataagattg taacaaatcg tatactagca ttacctattt ttgcacttat aatgtgggga
     4801 gtttactata tagcagtaag ttcattgggt actatagcaa ctgattggac aaatgatgta
     4861 ctgtttggag aaattattca aggcaatgta agtaattttc tagcttcttt aaatgtagca
     4921 gagtggttac aaggacttgt agttgatggt ctaattggtg gagttggagc agtactagga
     4981 tttgtaccac aaatcatgct tttattctta ttattatcaa tattagaaga ttgtgggtac
     5041 atgtcacgtg tagcttttat aatggataga attttccgta agtttggtct ttcaggaaaa
     5101 tcatttatac caatgcttat aagttcaggt tgtggagttc caggagttat gtcaactcgt
     5161 actatagaga atgatagaga tagaaaaatg actattatgt taactacatt cattccatgt
     5221 ggagctaaga ttccaataat agcattattt gcaggggcat tatttggagg tgcttcatgg
     5281 gtagctccat caatgtattt cttaggtatt gcaatgataa tcatttgtgg aattatatta
     5341 aagaaaacta gtttatttgc aggagaacca tctccatttg ttatggaatt acctcaatat
     5401 catattccaa gtgcaaaagg tgttttaata catatgtggg atagaggaaa agcatttata
     5461 atcaaagcag gaacaattat atttgttgct tgtggagtaa tctggttctt acaatcattt
     5521 aattggtcat tacaaatggt agatgctgga gatagtatat tagctagctt aggaaatata
     5581 gttgcaccaa tatttgctcc tcttggattt ggaaattggc aatcatcagt agcaacagtt
     5641 acaggtctag tagctaagga aaatgtagtt ggaacatttg gagttttatt tggtatatca
     5701 gatgcaacag agcaagaccc aacacttctt gcttcagttg caagcatgtt tacagtagct
     5761 agtgcgtttg catttatggc atttaatatg ctttgtgcac cttgttttgc agctattgga
     5821 gccataaaaa gagaaatggg ttcatggaaa tggacatgga ttacattagg attccaaact
     5881 ttaacagcgt atataattgc tcttcttata aatcaagttg gaagtttagt tttaggtact
     5941 ggaggaagta ttgctggggc aataatatct atatttatag cagtagcagt agtctttgta
     6001 gttttaactt attcaaataa aaatatgaaa aaagaaaaaa tgggtaaatt aagttatatg
     6061 aaaaattaat tcatataaat aaggagtgat gaatatggct acatttatta tagcagctat
     6121 tgtagtagta ttaatggcac tagctgtaat gtatatggtt aaaaattcaa aaaagaatgg
     6181 tagcagttgt ggatgtggtt gttctggatg ctcaagtagt aagagttgtc atagcgctaa
     6241 gagataaata ggataagagc ctgataaaaa gttaaattgc tttattatca ggttcttatt
     6301 ttttgtataa ataacaaatt tcatatttga agtaggttag actatgctgt aattttactg
     6361 tataattata tataaatata tgattaggag ggattattgc taaattaagc aatataagtg
     6421 aatattatgt ttaaatttga tgttacaaac gagcttatgt gtatagcaag gggaagtggt
     6481 aaattttttg caaaaaaagg tgctatggta gcctttaaag gaaattttaa ttttgaaaaa
     6541 ttgctgttag gtccaagtaa tggcggtgga ttgggaagag cattattagg tcatgttcag
     6601 aggtcattga caggagaaca aatgccaata atggtggttg aaggtgaagg tgaaatttac
     6661 ctagctcaaa atgcttacca tgtagatgta atatcaattg acccaggtga ttccatttat
     6721 gttgagagtg aaaacttatt ggcattttct gaacaattaa attacagtgt aaaattgatt
     6781 ggttcagggg taatatctca aaaaggtctt tttactacac atttagtaaa taaaacaggt
     6841 caaaatcaag atgttgctat aataacagat ggtaatcctc taattcttga aggaccttgt
     6901 tgtgttgacc cagatgcatt agtagcttgg actggaagag aaccatatcc taaagtatca
     6961 aaactatcat ggaagacttt tattggtcag acttcaggag aatcttatca tatggaattt
     7021 gttgaacctg gacaaatagt cattatacaa ccatctgaaa gattgtctgg cttaaaaaca
     7081 gattctacac cttctgatag aatgagttca ggtggattaa gacttggcat agattaaatc
     7141 ttatgatata gcttaattat tatttaagat attaaaaaat aaaaagggta gcagggatag
     7201 atatgttaaa aggagtttta tatgtttgag aaaattcata aaaggcttaa aggatattat
     7261 atttttttaa taattttaat tttatggaag atagtatgtg ttttgggtat atggagtagc
     7321 tatattctgc ctcctcctga aatagtctta gatactttta taaaaatgat atatgacggt
     7381 tctattttct taaatgttta tgccagtgta agaaggatat taataggttt tttaataagt
     7441 tcattgatgg ctataccact tggagtttta tttggagtaa ataaaaaaat gtatgaatat
     7501 ttcaaaccac tatttgaatt tatgcgaagt acacctccac tagctttggt gccaatgctt
     7561 attttatggt ttggtatagg tgaagaatct aaaattataa ttattatttt ggcatccttt
     7621 tttccaatat ttttaaatac attgaaaggt ataaaaaatt gtgacaataa attaatagaa
     7681 gtaggtaaat cttttaattt atctaaaaag agaatttttt ataaaataat aatccctaat
     7741 tctatgctgg atattgttgt tggacttagt ttagggcttg ggtatagttt tagggctata
     7801 attggagcag agcttattgc agcttcttca ggactaggat atttgatttc tgatggaaaa
     7861 gatatgtcta gaactgatgt tgtattggta ggaatttttg ttattggatt tttaggtata
     7921 ctttcagatt atctattttc aaagataata acaaaattta gcaaagggaa gcaggttgat
     7981 ttaaatggtt agaagtggat ttttgctaga gaatatatat aagaaatatt tagttgataa
     8041 taaagagcat ttagttcttg ataatatatc tttaaatata tctagtgaag agataactgt
     8101 tatattaggt gaaagtggat gtgggaaaac aacgctactt agaattttag cagggcttga
     8161 aaatgctact agtggaaaca tatatttttt taataatgat aaaaagtgta ctccaaaagt
     8221 aggaatggta tttcaagaaa gtagacttat gccatggctg aatgtaagtg aaaatatact
     8281 tttacatact gaaaaagata atagaaataa agttgattta gacaagtatt taaaaatgat
     8341 gaagcttgaa aagtttaaaa actcatatcc aaatgaatta tcaggaggaa tggctcacag
     8401 agtaagtata gcaagagcac tttccttcaa tcccgatatt ttgctgatgg acgaaccatt
     8461 tgcagcacta gattacttca caagaagaaa aatgcaaaaa gaagttgtaa atatccataa
     8521 aaatactaaa aaaggtgtag tatttgtaac tcacaatatt gaagaagcta tggaaatagc
     8581 aaagaaaata attgttttta gtaaaaataa aaggattaaa caatttagtg tcgaagatga
     8641 atataataga gatttaacta aaaactatta tataaacttg aaaaaagaaa tattaaggga
     8701 attgggggag ttttaattga ttaaaagaaa caaaaagatt ttagcagtaa tgacatcttt
     8761 aataatggtg ttggttggga ctgtaggatg ttcaacaaca aatgataaga aagatgaacc
     8821 tgcaaaaaaa gaggctagtt tacctaagga aataaattta acttatgtaa aatcaccact
     8881 taatgttcct agtatattag aaaaaaagga tgatttattt ggaaaggaat ttaaaaagga
     8941 caatataaaa gtcaatttcc atgaattgac aacaggacca gagcaaactc aagcattagc
     9001 agcaggtgag ctagactttt tacatgcatt aggaggaacg tctgcaataa tagctgcatc
     9061 aaatggtgtt ggtcttaaaa ttacgaatat ttatagccgt tcaccaaaag gatatatgct
     9121 tataactaat aatgataata ttaaatctat aaaagactta aaaggtaaaa aaattgtagg
     9181 tccaaaaggg actatacttc atcaactgtt gattgctgca cttgctaaag aaggctatac
     9241 tatagatgat gttgaatatg taaatatgga tattcctagt gctgcttctg cattagaaag
     9301 taaaagtgct gatgtagcaa tgcttgcagg tccagtggca ttgaaaacga taaattcagg
     9361 tgccaaaatg attgtaaatg gagaaggatt agtaagtgga ataatagtta ctgctgtaag
     9421 tgatgacttt gctgaaaaat atcctgaatt agtaaaaaga tttatgaagg ttcatgaaga
     9481 aactttaaaa tatatgaatg aaaacaagga tgaagtgatg gatgttgtat ctaaagaagt
     9541 tggtttatct ttagatgaaa caaaagaaat gtattcttgg tatgacttta gttctaaaat
     9601 aacggacaag gatataaaag aattagaaga tacacaggaa tttttgatgt ctaatggaat
     9661 gcaacaaaag aaaataaata ttaaagatat gttgtacaat caaaattaga atactttaaa
     9721 atattaaagt ggaaaataca cccttattta ttatggaaac tgtatggtaa aagagggtgt
     9781 atttttatta ttatacaaaa acacaattaa aaacagctta ctattttatc tacacttatg
     9841 tagtctagta attttctcat tttcttacgg tgaagtaaaa acttcgaata tgtgtcctcg
     9901 gattgttttg tgtcaccata aactttccaa tatttaatca tagaaacttc ataattacta
     9961 tatcccataa atccaatcac atctttaaga ggatatccta aaaaaatacc tatttcatca
    10021 ggaaaaacat cactcttaag tttatctact aaatgctcta aataagcatt aacattcgta
    10081 ttttgtttgt atcctaaaaa ctttaaaaag tttactgttt ttttacattt taatttttct
    10141 gataatgctt ttttatttac aaataagatt tttaatccct ttttttcttt atcaataact
    10201 ataaaatcta ttttcttaca tttatataga tatttataaa tatcattaat tttttcctct
    10261 ctatttacat cagtaaacga aatatttatt atttcagcag gtttagagcc taaaaagact
    10321 ggacctaaaa cttctaaaag ccattttata tatgatgatt caattttatt tttacaacaa
    10381 aattgtgaat tacaacaaga cataaaagat aacctccttc aaatgaaaat taatatcaag
    10441 taattagatt atactatgaa tgataatcaa tatcaataat aaaattataa tttttttatt
    10501 taattattta ttc
//
LOCUS       DAENCV010000130        10468 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAENCV010000130 DAENCV010000000
VERSION     DAENCV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.132"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            299..1723
                     /locus_tag="KQD82_003151"
     CDS             299..1723
                     /locus_tag="KQD82_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG4381606.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIDEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1758..3887
                     /locus_tag="KQD82_003152"
     CDS             1758..3887
                     /locus_tag="KQD82_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG4381607.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSRIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3917..4339
                     /locus_tag="KQD82_003153"
     CDS             3917..4339
                     /locus_tag="KQD82_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381608.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4358..5509
                     /locus_tag="KQD82_003154"
     CDS             4358..5509
                     /locus_tag="KQD82_003154"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG4381609.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5615..5716
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5899..6471
                     /locus_tag="KQD82_003155"
     CDS             5899..6471
                     /locus_tag="KQD82_003155"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG4381610.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6535..7185
                     /gene="fsa"
                     /locus_tag="KQD82_003156"
     CDS             6535..7185
                     /gene="fsa"
                     /locus_tag="KQD82_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG4381611.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7337..10261
                     /locus_tag="KQD82_003157"
     CDS             7337..10261
                     /locus_tag="KQD82_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4381612.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4289 a         1016 c         1955 g         3208 t
ORIGIN      
        1 aattaaatct atattagtat atccaaaagt acatttaata aatttattta actgaattat
       61 tattttcatt tctattaaat ttaaatattt tataaaaata gtattattta caaaatcata
      121 tactccaaaa tactacataa ataaaattaa acaaaaacta aaatgacaaa tgtcattatc
      181 taaagtgaca tagaaacggt ttcttaaagt tgttactata aaatataaac tctataataa
      241 aagtataaca aaagggaatc atatcgatat agaatataat tcaagaggag gtaaaattat
      301 gagtagttct aatcaatcga caataaagac atctatacag cgagtaggga aatttttaag
      361 tggtatggta atgccaaata taggagcttt tatagcatgg ggtcttataa cagcattatt
      421 tataccaaca ggatggatgc ctaatgaaaa attgagtact ataggtgacc caatgataaa
      481 atacctttta ccactattga tagcttatac tggtggtaaa gcaatagcag gacaaagagg
      541 tggcgtaata ggtgcagcag cagctatggg agtaattgtt ggtgcagata taccaatgtt
      601 tatcggagca atgataatgg gaccatttgc aggatgggta attaagaagt ttgataaatt
      661 tgtagatgga aagataccaa caggatttga aatgttagta aataactttt caataggtat
      721 aataggtatg cttcttgcaa tactaggatt ttatgcaatt ggaccagcaa tagtagcagg
      781 gacagctctt atagaatctg gagttcaatt tatagttagc aaaagtttat tgcctctagt
      841 ttcagtattt atagaacctg gaaaagtatt attcttaaac aatgcaataa atcatggtat
      901 attagggcct ataggtatag ctgaggcaaa agaagcaggt aaatctataa tgttcttact
      961 agaaagtaac cctggaccag gtttaggagt tctattagca tattggatgt ttagtagagg
     1021 aagtgtaaaa caatcagcac caggagctgt aataatacat ttctttggtg gtatccatga
     1081 aatttacttc ccatatattt taatgaatcc agttttaata ttggctacaa tggcaggtgg
     1141 agcagcagga atattgactt tttcaatatt aggagctggg cttgtagcag caccatcacc
     1201 aggaagtata tttgcactta tggcactagc tccaaaggga ggattacttc cagtattagc
     1261 aggagttgca gtggcaacag ttgtatcatt cttagtggca gcaccatttg ttaagagagc
     1321 ttcagcaaat caaagtgaag aagacagtac ttctttagaa gaagctaaag caaaaatgag
     1381 tgatatgaaa tctgcaagta agaactctga gaaaaatatt gacgaaaaac agcttgaagt
     1441 aaatgagatt aagaagattg tatttgcatg tgatgcagga atgggttcga gtgcaatggg
     1501 agcttccaga tttaaaaata gaataaaaaa cttagactta aatatagaaa ttacaaattc
     1561 atctgttgat aatcttccag atgatacaca aatagtagta actcacaata cattagttga
     1621 aagagttgct aagaataatt caagtgttga gatagtttca ataaacaatt ttttaaatga
     1681 cccaaattta gatacattgt ttaaaagatt agaatctaaa taaaaataaa aagtaccact
     1741 aattaggagt ggataatttg aaaaaaaaga agattacatc taggcaaaaa aagataatac
     1801 taatgatagt agaaaattca aagaaaaaca ttccaataac tatttctgag attgcaggga
     1861 ctttagagtt aagttcaaga actgtactta gagatatgtc tggaattgaa aaatggtttg
     1921 atgaaaatga ttttaacttt gtaaaaaaac caggagtggg attgatttta gaggaaaata
     1981 tagaaaatca aaactttata attgagttgt tagaagaaga aaaaatagaa aaagaataca
     2041 gcaaggaaga aagaaattta attatattat caaagctact agtttctaac gagccagtaa
     2101 aatcctatta ttttgcaaaa atattaaagg tatctgaagg ggtactgaat aatgattttg
     2161 ccttagcaag taaatggttg gaaagatttg atattgagct tgttagaaaa ccagggcttg
     2221 gtgtctattt aaaaggtcaa gaaaaaagct ttagagaggc atatgtaaat cttatatacg
     2281 attcattcaa tgaaaaagaa atactagata tggtcagaaa tataagtgaa aatatacaaa
     2341 cagataaagc aatagaaatt ttaagtgaaa atagactttt aaatttaatg gacaggtgca
     2401 taataagaaa agttgaagct acattaacaa agaagctttc agatttagat gtaaatttag
     2461 ctgatagtgc atatataggg cttgtagttc atatttcatt agccttacag aggataaaaa
     2521 atggtgaaaa tataactatg gataaagagt ttctaaaaga attatctata acagaagagt
     2581 ttaaattagc taaagaaatc gtaaaaggta tggaaacaga ttttaacatg gatataccag
     2641 tagatgaggt tggatatata accatgcata taagaggtgc aaagcaaagg tcatcttcaa
     2701 accataaggc tttaaactta gatgatatag aaataatgga aattacaaat aagatgatag
     2761 atttggcgga agatgaattt aaaataagtt taaaaaatga cgagaggtta tttaaagacc
     2821 tagcaaatca cttagggcct tctataaata gattgaatat gggattagaa ataagaaatc
     2881 ctcttttaga tgaaataaaa agcaagtatt catatgccta tgatggagtt gaaaagattt
     2941 ctagaattat aaaagataaa ctaaatataa attcaatacc tgaatcagaa ataggatata
     3001 ttgcaatgca ctttgcatca gccatagaaa aaaatcttat gatgaatact aatataaata
     3061 ttgttgtggc atgtcccact ggaataggta cttcaagatt cttatcaact aagattgaaa
     3121 ataaatttcc taatttaaat atacttgaaa ccatatcagc tataaacata gatgaagagt
     3181 atttaaagga aaaagatgtt gatttaatag tgtctacagt tgagttaaat acaagtttaa
     3241 attatatatg tgtaggtcca tttatgagtt tagatgatga acagataatc aaggaaaaaa
     3301 taaaatctat agctcaaaat aaattaatca atttaaatgc gaaaaatgat actaagagta
     3361 agaataaagt atatgaacag ataacagaaa gtatgaatat aggaaaagat attcttcaat
     3421 ttttagagga aattagattt gaaaaatttg aaagtaaaga tttaagtgag cttattgaag
     3481 actcatcaag aatatttgca aaatctagtg aagatataat ctctataaaa gaatcgttaa
     3541 aagaaagatt aaaaatctca attccatata tagaagaatc aaaaattcta ttgttacatt
     3601 gtatgagtga aagaatagat attatgaagt tatcaataat aaaactagag aatagaatcg
     3661 tgctagattc aaatgaagaa atcgacaatg tagtttttat gttacttcct aaaaattcac
     3721 caagttatca aagacaaata atgagtgaaa taagtggttc attaatagac aatataattt
     3781 ttactaataa gataaataaa ttctctatag aagagatggc attagaggta aaagatatag
     3841 tttttaattt ttatacaaac agattaaaag catttataga taaataaaat taatattaaa
     3901 aaggaaggtt gatactatga gtaaagtatt gaatgagaat aatatatttt tagggttaga
     3961 tagtgtttca aaggaagaag ctataacttt agcaggaaga aaattagttg aaaatggata
     4021 tgtaaaagaa gaatatatac cagcaatgtt agaaagagaa aaagtaatga ctacatatat
     4081 gggaatggga gttgctattc cacatggtgt aaatgaagca aaaaaagaaa tattatcttc
     4141 tggaatagtt atattacaat tccctaatgg tatagatttt gatggagaaa aagcatattt
     4201 attaatagga atagctggtg ttggagatga acatttagaa atactatcta atatagcagt
     4261 agttttagat gatgacttaa cagaaagact aaaaaattca aatgacaaac aagcttttat
     4321 ggaagctttt gccaactaat cttaggaggg atttaaaatg aaaaaggcaa ttcagtttgg
     4381 agcaggaaat attggaagag gttttattgg aggcttgtta gttaagtcgg gttatcatgt
     4441 agtatttgct gatgtaaatg aggatatatt aaactcaata aacaaagata aaaagtatac
     4501 tatacacata agagatgtag aatgtataga tgaagtaatt gataatataa gtgcggtttc
     4561 atctataaaa gaagaaataa tagatgagat tgtacaggct gaaataataa ctacagcagt
     4621 tggaccttta gttttgacta aaatagcctc tacaattgca aaaggtataa aggctagaaa
     4681 agaaaaggga ttaacaagta atctaaatat aatagcatgt gaaaatgcaa tatacgcaag
     4741 ttcttcttta aaagaagagg tacttaagta tttaaataag gaagaagtag aatatttaga
     4801 gatgtatgtt ggttttccaa actgttcagt tgatagaata gttcctcctg gtaaaaacga
     4861 aaatcctctt gatgtaacag ttgaaaattt ttatgagtgg aatgttgaaa aacaaggatt
     4921 taaaggggaa ataccaacta tagttggaat gaatttagct gataatctta tggcttatat
     4981 agaacgtaaa ttgtttacac tgaatacagg tcatgctata acagcttata taggatattt
     5041 aaaaggatat aagactatag aagagagtat aaaagataaa tttatctgtg atatagtcaa
     5101 aagtgcaatg gtagaaagtg gagaaggatt gattaaaaaa tacaattttg actcagaagt
     5161 gcactataaa tatattgata aaattttaaa tagatttaaa aatccatatc ttaatgatga
     5221 tgtattaaga gtcggaaggg aaccattaag aaaactaagt gataaagata gattgattaa
     5281 acctttaatg acagcaaaat catatggatt atcagttgat aatttgattc ttggaatagg
     5341 tgcagcgctt cattataata atagtgaaga tactcaaagt gtagaattac aagagttgat
     5401 aaagtctatt ggggtaaaaa aagccgttgc taaaatagct aatataagca atgacgaaga
     5461 acttttaaat aatatcgaaa aaagttatat atttatgaaa aatttataaa tttacttgaa
     5521 aaaaagtagt tctgataaag taactataat atattattct acacagtttg aattatttgt
     5581 tgaataatag agaattataa tatataatta aaaggaataa attaatatat ttacctgtat
     5641 atgtttgata atatggttca aatgtttcta ctaagctacc attaatagct tgactacagg
     5701 agtgaagaag atatagttta tacttatttt aaattatata ttaagttaat tactttgaat
     5761 tttaagctcc tgtctatgat atagacagga gcttttttag tattttaata taaaatactt
     5821 acaagaatag atagcaaaat tagaacaaat ataatttttt tattaatata attttagaaa
     5881 gggctaggtg gaagttcaat gaaggcttta caagaaaaga tattgagaga aggcagtgtt
     5941 tcaggaaatg atatactaaa agttgatagt tttttaaacc atcaaattga tgtagctttt
     6001 ttaaatgaaa taggaagaga atttaaagag agatttaaag gagaaaaggt ggataagata
     6061 tttacaatag aggcttcagg aatagctata gcatcaattg tttcacagta ttttgataat
     6121 gcacctgtag tatttgcaaa aaaatcagaa tctaaaaatt tggataaaga tgtatatgaa
     6181 actaatgtat attcatttac taaggcaaga gagtattcag ttaaagtttc taaaaagtac
     6241 ataaacaaag gcgaaaatat attgatagtg gatgattttt tggcaaatgg aagagctgct
     6301 cttggactta aagatttaat tgagcaagca gaagctaact tagttggagt aggaattgtc
     6361 attgaaaaag gatttcaagc tggaggagca cttttaaagg ctaatgatgt aagattagag
     6421 tctttggcag tagttgaatc tatagataat ggaactgtaa aatttaggta atttatgata
     6481 ttatatactt gtgtttagca catattttaa ctatgtaatt atggaggatt ttttatgaga
     6541 ttttttatag acacagccaa tattgaagaa ataaaagaag caaatgattt aggtgtaata
     6601 tgtggtgtaa ctactaatcc gtctctaata gctaaagaag gtagagattt tatagaggta
     6661 gttaaagaaa taagtgaaat agtagatgga ccaataagtg cagaagtaat aagtttagaa
     6721 cataaaggta tgatagagga agcagaaaaa ttatctaaga tacataaaaa tatagttata
     6781 aagataccta tgacagcaga aggtctaaaa gcggtgaaag tactatcaag taaaggtata
     6841 aagactaatg taacgcttat attctctgct ggacaagctc ttttagctgc tagagctgga
     6901 gctacatatg taagtccttt tgttggtaga ttggatgata tatcacaaaa tggtcttgat
     6961 ttaattgaag aaatcgttga tatatttagt gtaaatagta ttgaggctca aataatagtt
     7021 gctagtgtta gaaatccaat acatgtttta caagctgcta gaatgggagc agatatagca
     7081 acagttcctc ttaaagttat aaaccaaatg attaagcatc ctttgacaga caaaggaata
     7141 gatagcttta tgaaggattg ggaaggtgct tctttaaaat tataattata aactacgctt
     7201 tataattata ttaaagaggt tattatggaa attctaagta ataaggctag gctattttgc
     7261 ctagtcttat tttggtgtat aatatattga aacatattat tgaaatagat taatttttta
     7321 agtgaggagg cgaaaaatgg ataaaatatt aagagagata caaagagaag ataaaaaaaa
     7381 tccatatact gaccaagaac ttgctgaaat gttaaatgta gctaggtctg aagtgatatc
     7441 agttaggaaa aaaaataata ttttagattc gagagaaaga agaaaaaaaa tattaataaa
     7501 agatatatca aaaattgtaa gtgaaaatcc tcaaatgtct gaaagaaaaa taactgaaat
     7561 attaatagaa aatggatata atatatcgag aagtgcagtt tcaaaattat taaaagagga
     7621 aaatttaaat cagtcaaaga ttgggattaa ggataaagac ccagcatcat caggaaagta
     7681 tgtgaaggtg agtaaagttg tagaagaaga tggatttgaa gaattgatag gtataaaggg
     7741 aagtttgaaa gaaaaggtaa atctagctaa gtcagcaata atgtatcctc ctaatggcct
     7801 tcatactata atatatggag aaacaggagt aggtaaaagt gaattagcta catgtatgta
     7861 taaatatgct ataaaaaaca acattaaaga agagaacagc ccatttatag tgtttaactg
     7921 tgcagactat gcagaaaatc ctaatctatt gatagctcaa ctctttgggg tagtaaaagg
     7981 tgcatataca ggagcagatg caaatagaga aggtcttgta gaacaagcta ataatggaat
     8041 attgttttta gatgaaattc atagattgcc tgctgctggg caagaaatct tattttcatt
     8101 aatagataga ggagaattta gaaggttagg agaaagtagt tctaatagaa aggcaaatgt
     8161 tctcataata agtgctacaa cagaaaatcc agattctaat ttacttcaaa catttagaag
     8221 acgtatacca atggtaataa atttacctaa tatgagtgaa agaccaaaat ctgaaagata
     8281 tgaaataata ataaagtttt ttgaaagaga agcaaagcga gtaaacaaga attttataat
     8341 aactaaagaa gttatgtgtg cacttatgaa ttataagtgt actggaaata taggtcagtt
     8401 aaagagtgat atacaagtaa cttgtgctag agcttttagt aaatcgatat tttctagcga
     8461 taaggtaatt atagatttag atagtttaag ggattatata aaaagtggat attatgattg
     8521 taattgtaaa aatgaaaagt attctgagtt ttcagttgag gatatatata tagatattag
     8581 tgaattggtg agtggaaaaa atagaatttt atctgataat gagataagcg agatatataa
     8641 ttatgcagaa aaagagctta aggttttgga aagtaagtgc ttttcagaag aagagttaaa
     8701 aaatgcattt atagaaaagt tagataagaa gtttaatgct attaaaaaca ataaaaattt
     8761 atctgataga agaagtcgaa tagactgggg aggacaatta aaagagtcta cactggagat
     8821 aatggataag gtaattgtat ttattaagga gcagtttaag catgtaaatc aaggtctgta
     8881 cttggctcta gcaattcata ttgagcatgc tataggtagg ataaaagatg gaaaaactat
     8941 aataaatccg agtttagata aaataaaagc atcaatgcca atggaatatg agttgtcaag
     9001 atatataatg ggtatagtag aagatttgac acaagtaaca tttccagaag atgagcttgg
     9061 atatttggct tattatataa ataaattttg ttataatgag gaaagtataa aagataaagt
     9121 taaagtagta atagttacgc atggtaaagt tggaattgaa atgtctaaag ttgtaaatca
     9181 tattttaggt atagaatgta cactaggtat agaaatagca cttacagatt caccatcaga
     9241 ggggatagag catgtattag aagaacttca aaagatagaa gctagaaaag gtattctagt
     9301 tctaattgat atgggttctt tggtaattct tggtgatgag gttgaaaaaa ggcttggaat
     9361 tagatgtaaa actgtaaata gagtagatac attattggca atggaagcag gaaaacttgc
     9421 aaccattgaa gggaaaagtt tagatggtat aatagctgat ttaaagaaaa ataagaatta
     9481 tgctatgata aatacaaata aatttagtta cagaaaaaac gagtatggaa agaaaaatgt
     9541 aatcattact ttatgtttga gtggtgtagg tacagcttta aatttaaaag agcatataga
     9601 aaaacaaatt aaggaatatg acactttaat tgaagtaaag ccagttgctt ttttaaataa
     9661 taatttagaa gaagagttaa atgatattga atctgaatat aatattattg ctatctgtgg
     9721 aactattgat atagattata aaaacgtgcc ttttatttct tataatgagg tacttggtaa
     9781 aaatggtatt aataaagtat tagagcattt aaatagtaaa caaccagctt taaatattga
     9841 aaaccagttg aataatttaa tacatgaaga tttgatttta tacaattttg aaggaatttc
     9901 aaaggagtat ataattgata ctcttgtttc aaaacttgaa gaaggtggat atgttgactc
     9961 aaagtatata ttaagtgtat ataaaagaga agcaatggga agtgttgtaa tggcctctaa
    10021 ggtagccgtg ccacatggac ttccagaaaa cgttataaag cctgcaatag caatcgcgag
    10081 gttgaataaa cctatagtat gggacaataa atttatggtg gatttggtgg tactactggc
    10141 acttaaagaa aataataaga aagaaatacg aagcttattt tcaaagatta atgaccagca
    10201 tactttagaa gttttattaa atactgaaga taaaaatgaa ataaaagaac ttttaagcta
    10261 attattagta ccgagaagaa agacaaaaaa atttgcttta gtataaaatt taaaactact
    10321 caattgttaa gtagttaatt actctatata aggctagttc tatgaaattt atttatatga
    10381 ttttatagaa ttagcttttt tcttggtaaa taaaatatat gaatttattt atgtaaaaag
    10441 taaattttat tggacaattc tatgagaa
//
LOCUS       DAENCV010000131        10416 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAENCV010000131 DAENCV010000000
VERSION     DAENCV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.133"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(11..1939)
                     /locus_tag="KQD82_003158"
     CDS             complement(11..1939)
                     /locus_tag="KQD82_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4381613.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2173..2625
                     /locus_tag="KQD82_003159"
     CDS             2173..2625
                     /locus_tag="KQD82_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4381614.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2628..2936
                     /locus_tag="KQD82_003160"
     CDS             2628..2936
                     /locus_tag="KQD82_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG4381615.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3050..4156
                     /locus_tag="KQD82_003161"
     CDS             3050..4156
                     /locus_tag="KQD82_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG4381616.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENIEFDEIELDIM"
     gene            4231..6909
                     /gene="mngB"
                     /locus_tag="KQD82_003162"
     CDS             4231..6909
                     /gene="mngB"
                     /locus_tag="KQD82_003162"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG4381617.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7226..9559)
                     /locus_tag="KQD82_003163"
     CDS             complement(7226..9559)
                     /locus_tag="KQD82_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4381618.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKAISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTMTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9534..10250)
                     /locus_tag="KQD82_003164"
     CDS             complement(9534..10250)
                     /locus_tag="KQD82_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381619.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3875 a         1463 c         1359 g         3719 t
ORIGIN      
        1 atataaaaat ctatactata gacttgatta tggaaagaaa attatcaaaa tcattacaat
       61 tagatatact atctctaata tcattgtcat gaattatctt taaaaggata tcataaatgg
      121 agtttatttt tatataatca tctttattta tagccaacat catgattaat ttaacagtag
      181 aatcttcact ccaatatatt ggttcatcca atatggaaat acaaatctta gttcttatag
      241 agcttaattc cataggatga ggtatagcta aaaaatcatc tatattggta gaagataaac
      301 tttctctgta aaatacagat tcagcaaatg atggaaatac aatttcactt tgagttaatt
      361 tgttgcacat caactttata agctcatctt tatctttgat tttaggatta acaataaata
      421 aatttttatc aaagaaattg tccaaaagat tactataaat atgggaatta ttagtgattg
      481 acttagatat attctctata tcttttttta atagttttaa atccactaat acaataggaa
      541 tactatcatg atttaaagga attgtcgata ttattatatc tacatctgac aattcactag
      601 ctaaaaattg attaaatgat agacattgaa gtatatttat tttatcatga aaaactttgt
      661 ttaattgaac ctctaataac ctacttgaac cataccctga accacatact aatactacgt
      721 ttttacactt aatattactt tgaaaaaatc tctctattcc agcacctata tgcagggata
      781 catatccaat ttcatcttct gttaaagagt aaatactatt tttaaaaact ttttctatag
      841 catttaaagt tatttcaaat gctaaaggat aattactctt tattgtattt ataaggggat
      901 tcaccttatt taaattataa gatttagaat tgagaattga tttaaagtgc agtactaaat
      961 catcaaataa tacactatca tttctaaggt caaaagtata ttgattgttg attacttcta
     1021 aaatttcttc aacatactct ataattttag tatctaccct attggaaacg ttatccaaaa
     1081 gagaactttt agttataaaa tgattatata gataaactct gtctgccttt gaaataatta
     1141 tagtatattt gctctcaata taattaaata tattttcaac agttgtattc gattgaaaat
     1201 ctgttaaaac aacatctaaa acattatcta agcagtaacc atcaagtatt ctacttacag
     1261 taatagctaa atggataata aagtttttta gattaaaatc taggaaattg atattaaatt
     1321 tattaatttc acttaacact accctttgaa gctctattaa atctacatta ttaaataatt
     1381 ctatttctct atctgtaatt ccaattatat agttttcaaa attcctattt atcatattat
     1441 tagaaataca tcttcgaata tctatttcct tcccttcaac tttaactcca taatatggct
     1501 tagaaactaa tattaaatta tactttgaca aaataggctt tattcttttt atgtcattta
     1561 gtattgtaaa tttactcaca tacaagctat tagctaaatc ttctagtttt atatatgtat
     1621 tgttatagag aatatatttt aaaatatatt gatttctttc tataggagaa tctggtattt
     1681 cacttgaatc cataatatca tcagatatta atgctagata ctgttggtat aatgctaaat
     1741 taagaatatc tatataatat ccttctcctt tttttatttt aataatagca ccatttttct
     1801 ctaaagttcc atttatagaa gtaatatcat ttcttatagt tctctcactc acttttaaat
     1861 gattacttaa agttttacaa gtaattttat catcactttc atttaataat ttaaacaacg
     1921 tgagcaatct cttgtgcata aatatccccc cttatagaat ttcttctaaa ttatactatt
     1981 tacactaata taatacaatt actctaataa aattgtatta taataattta tttccacttc
     2041 tatatggaaa ttagttatta tcataatata aatttccatg tcgaagtgga aattagttat
     2101 atttaattta attaggataa tgattacaat ataaatataa gttaagacat tatgaatgga
     2161 gtggaaaaat atatggtact acaagaaaaa tatatattta tagataaaga cttaaaaagc
     2221 aaagaagaag tattaaattt tattgcagat agtgcttata tactaggtat tacaactaat
     2281 aaagaggctc ttttaaaaga ccttctaaaa agagaaaatg aatttccaac aggaataaaa
     2341 gatggatttg cgatacctca tacaaagagt gtaaatgtaa aggaagcttc catactctat
     2401 attaaaacaa ctaacgagtt agagtggaaa actttagatg atagtaaggt tagatatgta
     2461 tttagtttgt tagccccaca taaaaatgaa ggaaatatac atttgaaaac tctatctaaa
     2521 ttagccacat gtttaatgga ggatgatttt acaagtgaag ttaaaaatgc tagtgatgca
     2581 agtcatttag taaaatatat aactaataaa atgaaggagg aataattatg aaaatagtag
     2641 caataacatc atgtatagca ggattagcac acacacctat ggcagcaaaa gctttagaac
     2701 aagctgcatc aaaaatgggt catgaaatca aagtagaaca acaaggtgct atgggacgag
     2761 taaatactat aacaaaagaa gaagcacaag atgcggactt tatacttata gcatctgacc
     2821 aaactatatc tggaatggaa agatttgatg gtaaaaaagt gataaaagtc aaaataggta
     2881 tagcgttaaa aaaaccagaa gctgtcttaa caaagtgtat agaagctatt tcttaataaa
     2941 aaaatctcac tctaatatag aatgagaaaa atcaaaaatt aaaaaattgt ctaatataat
     3001 tataacatat tatcaaaaaa tataacaaac ttatatagga ggattaaaaa tgagtaaaaa
     3061 aaataaatct cttttaagtg aacttaaggg acacttaatg acaggtatct catatatgtt
     3121 gccacttgta ataggctcat ctttagttgt agctatccca aagcttacag ctttagcagt
     3181 gggtataaca agtttagatg cttataaaaa tgtatctggt ttttatcata ttctttactt
     3241 aatggaacaa gttggttgga ctggcatagg tctattaaat acggtattag caggatttat
     3301 agcattttca ataggtgaga aaccagctat gggtgcaggt tttattggtg ggctcatagc
     3361 aagtaatact aatgcaggat tcttaggagc agtaatagca ggtttctttg ctgggtatgt
     3421 ttgtaagttt ttaaagaaaa aaattaaaat atctgggtca gcatcaggaa tgatgccact
     3481 tataataatg ccacttataa cagttggatt aactggtttt ttaatgagtg ttgtattagc
     3541 aggaccttta ggaaacataa atacttcatt aaatgcttgg gtagctgaaa tgtgtcaaaa
     3601 tggaacaaat tcggtggtat tggctttaat tttaggcgca atgataggat tcgatttagg
     3661 tggaccagta aataaagcag cttggatggc aggaaatgca ttattgttag agggaatcta
     3721 tctaccagcg atattggtaa actgcgcaat agttatacct ccactaggat atggtttagc
     3781 tacattcata agaaaatcaa aattttccaa tgcattagct gaaacaggaa aaggcaatat
     3841 agttatgggt ataattggaa taacagaagg tgcaattcct ttcactttaa caaatccatt
     3901 aaaattagtt cctgttaata tgataggatg cgctttgggt gtaggattat cagctttgtt
     3961 aggtgtacat gctataatgc ctccagttgg tggattatat ggatttatat caataggttc
     4021 tggatgggct tacttagtag gtgcaatatt tggagcttta gtaatagcaa tactatcaac
     4081 aactttagta gacttcaatg aagatgaaac tacggaaaat atagaatttg atgaaataga
     4141 gttagatata atgtaaatta aaaacttaat tttagataat tgagaattga gtgtactctc
     4201 aattctcaat taaattttgg gaggattttt atgaaaaaga caaaagtaca tgtagttcca
     4261 cacagtcatt gggatagaga atggtatttt acaacatcaa ggtcaaaaat atatttaatg
     4321 aaagattttc aagatatatt agacatattg gaagaaaaag aagactttaa atactttact
     4381 atggatgcac aagcttctct cttagatgat tatttaaaat ggagacctca ggatgaatat
     4441 agaataagag atttagttca aagaagaaga ttagttattg gaccttggta tactcaaaca
     4501 gaccaattgg ttatatcagg agaatctata attagaaacc tttactatgg aatagataga
     4561 tgcaatgaat ttggtgaacc tatgagggtt ggatatgtac cagattcttt tgggcaatca
     4621 gctcaaatgc cacaaatata cagaggtttt ggaatagata gttcattatt ttggcgtgga
     4681 gtttctgatg atatggtaga tacaacagag tttatatgga aaggttctga tggttcaaaa
     4741 gtattagcag ttcaaatacc atttggttat tactatggag gtaatatacc agaagatgat
     4801 tcagatttaa aagaatattt aaaagatata atcggaaaac ttaaggttaa agcttctact
     4861 aataacgtat attttccaaa tggatttgac caagcaccaa taagaaaaaa ccttcctgat
     4921 atattaaaaa aagctaatga aatagataat gaaaatgaat acgaaataag tgatatagaa
     4981 aactatatag attcagtaag aaatgaaaga aatgaattta cagagcttaa aggtgaattt
     5041 ttaaatgcta aacatatgag aatacacaaa tccatattct cctcaaggtc agaccttaaa
     5101 ataatgaata acaaattaga aaattatatt acaaatgtac ttgaaccatt attaacatta
     5161 agttattcac ttggaaatga ataccctcat ctagttataa aagatatatg gaaacttatg
     5221 tttgaaaatg cagctcatga cagcataggt tcttgtaata gtgacacaac aaatgaggat
     5281 gtatacacaa gatacaaaca agccagagat ttgagtataa atttattaga tttgcatatg
     5341 agacttataa gtactcaaat caaaaataat aatgaagaaa taacattaac agtattcaat
     5401 ggattcccag aagtcagaaa tgaagttgta gaatttgata cttatattcc aggtcaagat
     5461 tttgtgataa agaataaaaa tggagaaatt cttaattata taataaaaga aaaagaagat
     5521 attacaaatt atatattaac tcaaacaata agattaaacc caagtaaaaa aatatatata
     5581 cccaaaactg tatatagagc aaaaattgca ctttcagtaa aagatatacc accaatggga
     5641 tatactcaac tagtttttga attggatact aaaaataaat gtgaaataga aacaagtaat
     5701 tgcaaagaaa tagaaaacaa atactacaaa ataacagcca ataaaaatgg ttcattagaa
     5761 atattagata aagaatctaa taagacttat aaaaaccaag ctgtaattga agataatgga
     5821 gatgatggag attcatacaa ctattctcct ccaagaaaag atatttatgt ttcatcttta
     5881 gattctgtat cttcagtaga agtgctaaaa tcaaacattc aagaagaaat gatacttaaa
     5941 tattcattga ccataccagc ttcactggaa gaaagggcta taggtaaagt ttctgtgaaa
     6001 atgccagtaa caatgaaaat taccttagaa cctaaagaac aaataattaa gtttaatgtt
     6061 aatataaaaa ataaccaagc actatctcat agagtaagag ttttatttaa tacagaaata
     6121 gcatctaagt tttctatagc agatcaacaa tttggaataa tacaaagacc aacagtatta
     6181 gaaaaagacc ttgcattgtg gaaaagagac aattactcat ggcaagaaaa acctataact
     6241 atcgaaccaa tgcaaagttt tgttacttta gaggatggac aaagaggaat agcattaata
     6301 actggttgtg taagagaata tcaaatagtt ggtgataatt tagatacaat agcattaaca
     6361 ctattcaggt catttggata tatgggaaga gaaaatttac tttatagacc aggaagagct
     6421 tctggagaaa aaataatagc aacaccagat gcacaattac ttaaagagtt aaactttgac
     6481 tttggtcttt acatatataa caataaattt gatgaagcaa atgtagctaa tacagctaag
     6541 agattcttaa cacctataca aatatatgaa tatgctgatt ttttaaatgg tagacttata
     6601 tttgcattca atgacgaaaa acaaatatat gaaaatgaat atagtttgat gaatgtaaat
     6661 aattcaaatt tcacagtaag tgcaataaag aaagaagaaa aaggagatgg aattgtagtt
     6721 agaatcttca atggtatgaa aaatgaggat gctaagggaa gtcttaacat caataaaaat
     6781 gtcaatgatg cttatagtgc aatgctcgac gaaatctatg ataatactag caagcttaaa
     6841 gtaaatacta aaactgtaga ggtaaattct ttatcacatt gtaaagtaca aacaatagta
     6901 attaaataat ataaaatagc ttatacaaga tttcaaaata aattgctagt actatgttaa
     6961 tagtaaaccc agatattatg ctatttataa gctctgtaac actaaataac attttgtaaa
     7021 ataaaaaggt gaaccttgta tattaggatt cacctttttt aaataaatca acattctgtt
     7081 ttaaaataac atttataagc tgttttgaaa taatgcttac aaattatttt aaaacaatac
     7141 ctataaattt ttatttaaag aatacacata aatatttcca gactctttta ttctatagac
     7201 tttataatta ttaaataagt ctaatctaat ttttgttaaa agtaattgta actttaaata
     7261 aatcaccatc cacacttata ttcatcttac caccctgtaa ttctataaaa ctttttgcta
     7321 tcgctaaacc aagaccagaa ccttctgtgt tacgtgattt atctcctctt acaaatctct
     7381 ccattatatc atctacacta aaatcaatct cagatgcaga catattttta agcataatat
     7441 ttactttatt atctgtttct aaaatatcaa tatacactct tgaatttggc attgcatatt
     7501 tgcttatatt tattataaga ttttcaaata ctctaaatgt acgctgacta tctaatggta
     7561 atattatttt ttcattagaa aagttattct ttactattag agatgaatct gcaagtttgt
     7621 catctaattc taaaagtgtc tgcttcatta gagatacaat atccacattt actatattca
     7681 aataaacatc tccactattt gccttgctta cttcaaacaa atcttctatt agatgttgca
     7741 atctttgtga ctttctgtct aatgtatcaa tatacaactt acgattttca tctgttatac
     7801 tttcatcttt taataaatct atataagtga ttatagatgt tagaggtgtt tttaagtcat
     7861 gtgatacatt tgatataagc tctgttttca tcttttgact cttaacctct tcgtcaacag
     7921 cttttttaaa tccagtttga atatttccta attcgtcttt tataggatta aaaaatccta
     7981 aatctttttc catatttaca tctaagtttc catctgaaat tttctttgta acatctaaaa
     8041 gttgattata ttcatatatt gtctttttag agtaatctga cagtatctta aataatacta
     8101 ctgaatatat taatgcgagt attattccaa agaaccacat actacacatt ataactataa
     8161 tcaataaatt tattcccaaa attgtaacta tagttttatt tggcttttct tttagattta
     8221 ttgttctaat ttgattaata actgtttttc ctgctttttt tagccataca aaaaacattc
     8281 ctgtcatagt atgttttgta aaatactctc ttattccaac atttaaaatg tgttttaaaa
     8341 gtattataaa tacaaatatt attgagaata ttacaaacca ataagttaaa ttcattaaac
     8401 ttaccaatat gctagccatt ttagcactta tgtcattgtt tataaggtac tctacataat
     8461 tcccactcaa ggtattactt attataacag taggatataa atctacaagc ccctgtataa
     8521 tagcaagagc aataaagagt atctctaacg gtattcttga taatgtatca aatcctagca
     8581 actctttaga ttttctatat gggaatgcta aagctacaat aacaactata gctaatataa
     8641 tttttaaaat cattccagaa atcttataat aagaattatt ttctacattg cttatatatg
     8701 aaattatacc atcatcaaaa taagtatttc cataaccata ctctacacta tcacctttac
     8761 ttattgcttt aggtacagca tatactaatg ttatattttt tattggagat aacttatatg
     8821 aaattgaatt tcctcctaaa cttgtatcca ttaaatcttc tcttgcttta gataacaaca
     8881 tttgattaat aactttttga tttactccat aagaatctac aatatttact tttccatctt
     8941 tatcaaaatt cattacaacg tagaatctat agttttcttt tatatctgta atctgctttt
     9001 gtctttcctc atcattcatt tctgtatttt ttgactgatt ttcatcagaa ttttcttttg
     9061 aagcatcatt ctttgttgta tcaagtactc ctaattcttt attatcatta aaaaattcaa
     9121 aattgctttc tttatcaact aaatagtact ttaaattctt tcctgacgct ttaatgaaat
     9181 gctcccaatt ttcaaagttt tcattaaaat ctgcattaat actatcatct gtatagtaag
     9241 tttctgaacc atcataagta tctttttctt tacttacatt ttttatatag ttcatagttt
     9301 cttcattgat aaatagcttt gatggttgta tcatcttacc ttcttgttct tccttcatat
     9361 tatgatataa aacataactt cctttatata tactatctac aaagctttcc tgttcaaatg
     9421 gattctctgc tccaccttta gcacttgctt gtatagtaga atattgacta atcataccta
     9481 atgatgctga taacataagt ataaaaacta taatagtaac tatatattta tctttaaatt
     9541 ttttcaattt tgtatccaac accccacacc acctttaaat atttaggatt ttttgggtca
     9601 atttctatct tttcccttat attcctcaca tgtaccataa ctgtatctgt atttacagca
     9661 ttctcattcc aaactctttc atatatttca tcagcagaaa aaactcttcc tgcatttctc
     9721 ataagtaaac ttagtatttt aaattctata ggagttgttt ttatatactt tccatcaata
     9781 ctaacttctt ttgtattttc atttaattct aatccgccta ttgcaaacac actatcgtca
     9841 ctttctacct tttgctcttt atttccaacc atgtttagat acttagtgta ccttctaagt
     9901 tgtgaattaa ctctagccaa tagttctaat ggcttaaaag gttttgttac ataatcgtct
     9961 gctcctatat taagccccat tattttgtca acttcctctg attttgcaga tagcattatt
    10021 actggaaaat tatagttttc tctaagcttc attgtaagtg ttataccatc cattttaggc
    10081 atcattatat caactatagc taaatgtatt tcttggtttt ctataacctt aagaccttct
    10141 aatccattgt atcccttaaa aacattatat ccttgattta atagatatat ttctatagca
    10201 tctgctatct ccttttcatc ttctaccacc aaaatattat atgaattcat tctaatctcc
    10261 tttatattaa tttataaatt ttctttacat atatgattat acaatatgat agaagttttt
    10321 ccatttatat atatatttat atcttttgtt ctggttattt gtatttaatg tccatcttag
    10381 aaacctcctt ctagtttaaa tttaatattt tttcat
//
LOCUS       DAENCV010000132        10399 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAENCV010000132 DAENCV010000000
VERSION     DAENCV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.134"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            131..2632
                     /locus_tag="KQD82_003165"
     CDS             131..2632
                     /locus_tag="KQD82_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG4381620.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAEAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2792..3460
                     /locus_tag="KQD82_003166"
     CDS             2792..3460
                     /locus_tag="KQD82_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381621.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3444..4721
                     /locus_tag="KQD82_003167"
     CDS             3444..4721
                     /locus_tag="KQD82_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG4381622.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5122..5343
                     /locus_tag="KQD82_003168"
     CDS             5122..5343
                     /locus_tag="KQD82_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381623.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EIKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5632..6396)
                     /locus_tag="KQD82_003169"
     CDS             complement(5632..6396)
                     /locus_tag="KQD82_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG4381624.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6670..7344
                     /gene="fsa"
                     /locus_tag="KQD82_003170"
     CDS             6670..7344
                     /gene="fsa"
                     /locus_tag="KQD82_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG4381625.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7466..8860)
                     /locus_tag="KQD82_003171"
     CDS             complement(7466..8860)
                     /locus_tag="KQD82_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG4381626.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLET"
     gene            complement(8938..10284)
                     /locus_tag="KQD82_003172"
     CDS             complement(8938..10284)
                     /locus_tag="KQD82_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381627.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         4228 a         1233 c         1614 g         3324 t
ORIGIN      
        1 tgatttaata tacctatagg gggtataata aaaatataga tttctaaata agtaatttat
       61 caatattaca tatcaaaata aaacgttaca aattagctga aaatatattg aaagaaagga
      121 agattttatt atgagttcaa ataaagtggt agaaagttat aaaattacag gtatgacatg
      181 tgcagcttgt gcaaaggcag tagagagagt aactaagaag atggatggag tatatgacca
      241 aagtgtaaat atagctactg aaaaacttaa aatagaatat gataattcta aggttaattt
      301 tgatgatata aagcaggttg tagaaaaagc aggatatgga attataaagg aagaaagtaa
      361 taaaaaaatt gatatgaaaa ttgatggaat gacatgtgca gcttgtgcaa aagctgtaga
      421 gagagtggta aaaaaattgg atggagtaga aagtattagt gtgaatattg caacagataa
      481 agctaatata gactatgacc catcaaaagt aaaattatct caaataaaag cagctattga
      541 aaaagctgga tataaaccaa tagaagaggt aagaaataag gttgatgtgg atgaagataa
      601 attgagaaaa gaaagagaaa tgaagtcttt atttgttaaa tttatagttg caatagtatt
      661 tgcagtacct ctgttttata tagcaatggg acctatgata ataaagccta tagggccatg
      721 gcctttgcca gaaattataa atcctatgac aaatactttt aattatgcat taattcagtt
      781 aattttagta ataccagtaa tgatagctgg ttataaattt tatataaatg ggtttaaatc
      841 acttttttct ttaagcccta atatggattc tttagttgca ataggaacat tagccgcatt
      901 tttatatagt ctatatacga cactacaaat agccaatgga caaattcaag gtatgcacca
      961 tcatcagttg tattatgaaa gtgcaggaat aataatagca cttatcttac ttggtaaata
     1021 tttagaatca aaatcaaaag gaaaaacatc agaagcaatt aaaaagctta tggggctaca
     1081 acctaaaact gcaatagtgt tagttgatgg taaagaagtt gaaactccta tagaagaagt
     1141 agagatagga gatatactat tagtaaaacc aggaactaaa atacccgtgg atggagttgt
     1201 aatagaagga tatacatcag ttgatgaatc aatgcttaca ggtgaaagta taccagtaga
     1261 aaagaatgtt ggaagtaagg tgacaggagc tagtattaat aaaaatggtg ttattaagtt
     1321 taaggctgaa aaaattggtg gagatactgc tcttgcccaa ataataaagt tagttgaaga
     1381 tgcacaaggt acaaaagcac ctattgctaa acttgccgat actgtatctg gatattttgt
     1441 accaatagta attgcaatag ctgtagttgc atcattattg tggtttttaa taggaggaaa
     1501 agatatagta tttgtactta ctatatttat atcagtactt gtaattgctt gtccttgtgc
     1561 attaggatta gcaacaccaa ctgctataat ggttgggact ggaaagggcg cagaaaatgg
     1621 aatcttgata aaaggtggag aggcactaga atctgcacat aaagtaaata cagtgatatt
     1681 tgataaaaca ggtactataa cagaaggaaa gccaaaagta acagatatag tattaaataa
     1741 taatgtaaaa gaagaatatc ttatcaagat tgcttcaagt gcagaaaaag gttcagagca
     1801 tcctcttgga gaagcaatag ttaagtatgg agaagagaag aatataaaat ttgaaaaagt
     1861 tgataatttt aaagccatac caggtgctgg tatacaagtt actattaatg atgaaagtat
     1921 attacttgga aatagaaagc ttatgaatga caataatata aagcttgggg atttagaaga
     1981 aaaatcaaat atattggcaa gccaaggtaa aacacctatg tatatagctg tagatggaaa
     2041 tttatcaggt ataatagcag ttgcagatgt agtcaaagaa agtagtaaaa aagcaataga
     2101 aatacttcat gatatgggaa taaaagttgc aatggtaact ggagataatg cgaaaactgc
     2161 aaatgctata gcaaatcaag taggtataga tatggttttg gcagaggttt taccagaaga
     2221 taagtcaaaa gaagttgaaa aactacaaaa tcaaggaaag tttgttgcaa tggtagggga
     2281 tggaataaat gatgccccag ctcttgcaaa agcagatata gggatagcaa taggaagtgg
     2341 aacggatgta gccattgaat ctgcggatat agtacttatg aagagtgatt taattgatgt
     2401 tccaacagca attaaactta gtcatgaaac tataaaaaat ataaaacaaa acctattttg
     2461 ggcatttgga tataatacaa ttggtatacc tgtagcagca ggaatattat atgtatttgg
     2521 aggaccttta ttaaacccaa tgattgcagc agaagcaatg agcttaagtt cagtttcagt
     2581 cgtatcaaat gcacttagac ttaaaaactt caaggcatat aagagagatt agattaataa
     2641 attaaataaa atgtatccaa aagagctatc tttaaattaa aaggattaat ttacaaatta
     2701 agatagctct tttttcatgt aaaattcaca acaataagat aaaatataag taatatacga
     2761 aattttaaat aaaatagaaa ggattgatag aatgaattcg tcaatacttg taattgaaga
     2821 tgattcaaat atacaagagt taatatcaga atttttaagt gcagaaggtt atcaagttga
     2881 tacagcaaat gatgggttgg aaggtataca aaagtttaag caaggaagtt atgatttagt
     2941 catattagat ataatgatgc caaacttaga tggttatgga gtttgtaaaa tgataagaaa
     3001 gtcatcgagc gttccaatta tatttttaac tgctttaaat gatgaaggag accaacttaa
     3061 agggtttgac ttagaatgtg atgattatat aacaaaacca ttttcattta atcttcttat
     3121 aaaaagagta gaagcaattt taaggcgaag taacaagact ataaatgaca aatttatagt
     3181 ttttgaaaaa ttaaaattag atttaaacac atatattgca gagatagatg gagaacctat
     3241 agaacttaca ctaaaagagt ttaatatatt aaaagctttg atagaaaagt atccacaagt
     3301 tataactagg gaaggtcttt tggatagtat atggggttat gactattatg gagatactag
     3361 gattgttgat gcacatataa aaaatataag aaaaaaaata tcattaccat atattaaaac
     3421 tgtaaaagga ataggatata cacttgagaa ggatatttga taagtgggaa aagttaagta
     3481 taaagtataa gttattttca ataacgacat cattattaat agcattagct ttaataatat
     3541 atctaatttt atatttttta ttgccatcat attatcatga gtataaaatt gagtctcttc
     3601 aagaaagttt gaaatcttta gtagatagtt caattcattt cgatacatat actttagaag
     3661 aaagacttta ttatatggct aaagaccaaa atttggctat tttattaaaa gataaccaag
     3721 gtaaaattgt atatggtaag aatgaagtag ttattttaag atatagtaag tacatgatta
     3781 atagtcttga ggatgagtat agaacttcta tacctatata tactaaagat gcaaaagatg
     3841 gtccatatac tttagaactc gttatgccat tacaaccaat tgacgaagca aacgaagtta
     3901 taagaaaact aatgccatac atcatatcaa tagcaatatt gatagcgatt attggagcat
     3961 atatatattc aattgtaata acaaagcctc ttataaacat aatagagagt gagagagaac
     4021 aggaatatag aaggaaggat tttgttgcta caatatcaca tgaactaaaa actccaataa
     4081 ccataataag tggtcaaatt gaaggtatga tttatagtgt aggtaagtat aaggataggg
     4141 atacttatct gaaaaaatcg tatgagtgta cacaagaatt aaaagattta gtaaatgaaa
     4201 tgatagaggt ttcaaaatca gaaatacttg aaaaagattt gaaattggta tcaataaata
     4261 taagtgaatt attaaataga cttgtaaaga gacaagtatt tttaatagaa gaaaaacata
     4321 tgaaaactat tttaaaaata gaagaaaatt tggaaattaa ggcagaccaa gagagaataa
     4381 ctaaggcaat caataatata ataaataatg ccataaaata ttctcctgaa gaatcagagc
     4441 ttataattag actctatgat aaaaataaac gaataagtaa aaagacttct aatcaaagag
     4501 tggttttaga aattgaaaat actggtgtaa ctatcgagaa acgttattta gaagaaatat
     4561 ttaatccatt ttatagaata gaaaaatctc gttcaagaaa aactggagga agtggacttg
     4621 gactttatat agtcagccaa atttttaaaa gtcatgggtt tgattatagc ataaaaaaca
     4681 aagaaaactc tgtagtattc acagtagaat ttaaaaatta attacttatt ataaagaata
     4741 tataactttc aataatagtt cattttaaaa tcacaaaaaa ttcataatga aactgtatta
     4801 tataaatata aagaagctta aaaccataaa atacaccaac tataatgatg agatttcctt
     4861 acatcctcca tataagtcat cattatagta acccaacaat taagttacta tgaatttaaa
     4921 aaataacacc gagcatataa tctatgctta gtgttatttt tattttgaaa taaaaaatat
     4981 ttaatttatt gaaatgtata gatttagata gtttaaacta tatttaagac ttaaaaatta
     5041 taattgatat tacaaataaa tatatgtact ttgggaaaaa taaatatagg aagtattata
     5101 tttacagagg aggtagtttt gatggataga ttaaaaagag atatgaaaaa tatgaaagaa
     5161 gatttaaaag aaggtgttaa aaagacagct tatgttatga aaaatacagc tgaagatttg
     5221 aaagatgatg cacaaggtgc tatggtaaac ttagaaataa aaaaagatga aatgatggaa
     5281 agatatgaac aaaagaaatt ttctaaagaa ttagagaagc aaatgaagaa tgaaatgaaa
     5341 taagctttta tttatgaata acaaattatt gtattcatat aaaaagtagc cagtaatatt
     5401 aaatagttta tttaaggaaa tagactttct aaaaataact gtattagatt tagaaagtct
     5461 atttcaaatt agttttataa atagacataa ggataaagta gatgttatag aactagattt
     5521 taaaaaatag atactaagga taaaataaat attatagaaa tatgttttta agaatattta
     5581 tcaaataaaa atttatattt ataagaaatc ctataaaaca tataattaaa cttataaaac
     5641 atttacatta aattctgata aaatatctac tatatcagga gtaggcgttt tttctgtaat
     5701 tatagaacta atatcttcta aatgagcaaa cttgaaattt ccatcaaatt taaacttttt
     5761 atcttccata actataaata cttttttact tgaatttatt atagccttct tcgtaagacc
     5821 atcttcaatt tcaaaagttg taagtgaaga gttgaataca tctactccac aacttcccat
     5881 aaaagtctta tcaaacttat atttcgatat aaactctata gttggagtac cgacaaaacc
     5941 gtctaaactg agatttaata aacctccagt ggaaattata tttaaattat ttggttttga
     6001 agtaactaca tttagtatat ccagcatatt tgtaacaagt gttacttttt tattactttc
     6061 agctaacaac ttcgcaagca agatgttaat tgtagagata tctaaaaata tagtttctct
     6121 atcttcaatt aaattaaatg ctttttctgc tattattttc tttgttggaa tattaatttc
     6181 ttttctgtct tttgtatcct gattttctag tgtttctcta gctagtacag ctcctccata
     6241 aattcttctt attgaagaaa tcttttctat attttttaaa tcttttctta tacaatcttc
     6301 tgtaacatta aatttagaac tgagctcttt gacagtaact ctaccttctt tttttaggat
     6361 atttaatata gcttggattc tttcttctgc aaacattttc tacctctttt cgcaatatag
     6421 tattatagta aatattataa gataaaatac aatatatcaa ctattttaac atgaaataca
     6481 aaattaattt aatataaaaa gtatgttttt tctaactata tatttagaat ttagtaaata
     6541 aaaaaattat aaaactaaga aggaaaagta aaacaataat aaaaaataaa aaacaataag
     6601 caaaatgttg aaaataatac caaatcaata tataataaga ataaagaaag attaaagggg
     6661 gatattttaa tgcaactaat cttagataca ggtaatgtgg aggagattaa ggagttatgt
     6721 acttgtttac caatagatgg tgtaactaca aatccaagta ttgtttcaaa agaaaaaaag
     6781 aattttaagc aacttataaa tgaaattggt gagattatag gtgaggatat gcctatacat
     6841 gctcaagtgt taagtacaaa gtatgaagaa atattagaag aagcattata tataagtagt
     6901 ttgagaaaaa atatatatgt aaaaatacct gtaacacaag atggtttaag agctataaaa
     6961 gaccttcaca gaaaaggtgt aaaaataact gctactgcaa tattcactgc tcatcaaggt
     7021 tttttagctg caaaggctgg agcaaattat attgcacctt atgtaaatag gcttgataat
     7081 atatctggtg atggagtagc tatggtttca gaacttataa aaataataga tacttataaa
     7141 atggatacaa aagtacttgc agccagtttt aaaaatgcac aacaagttat agaattaatg
     7201 caacatgggg tgcatagtgt tacagtacca tatgatattt gtaaatctat gatgaatcat
     7261 ccattaactg attggagtgt agataagttt atagaggatt gggagaatac ttttggaaaa
     7321 ggaagtaaaa caaataatat ttagtattat ggtaaaagag tagttttgtc caatttcaca
     7381 agaacttata taaaaagatg aaaatatgaa ttttcttttt aataatttcg ttattatctt
     7441 aagttaattc atattttcat cttttttagg tttctagtcc tataaatttc caccaaggga
     7501 atgttataca tattattata aagtaggtta aaggaatcaa agttaaaccg tatttgaaaa
     7561 taaccttgct attatacaga ttttcaccat gaccaaccat tattgtagca tgatgaaatg
     7621 gtaatatgta ctgtatgctt actataatgt agacaaatag acataaagta acactatttt
     7681 cctttagtat atgtaaactt ccaagtaatg gtattactac agatgatgta gtgacagcac
     7741 ttccaagaca tatattcaag attattgtga gagttatcaa aaacaatatt gaaataattt
     7801 ctgaagagtt tggtataaac tttatcaaat aatttcccat tatatctaca actccagagt
     7861 actttaacac tcctccaatt gaaaatgctg ctataaaaaa aataaggacc ttccaattta
     7921 tttcttttaa agtactaaat ccaactattt ttcttagtaa taaaagaagt acacaaattg
     7981 acattataat taatgtgttt atgttatgta tgaactgagt taaaaacata aaaaacatta
     8041 ttcctaggca taaaaatacg tgtttttgtt ctttgtttag caaattaaat tcttttttta
     8101 aattagaata tgaaaaatga agtttattac cctcaagatt ttcattagaa ataaatttta
     8161 ttgaatgcaa ttctttttta aagacaaagg taaataaaaa ataagttata aaacaagtaa
     8221 ttatagttgg aacagacata taaaaagccc attgacccca atttatatta caatttccaa
     8281 gttctagtac aacataattt agaagagtgt ctccatttac aaaaaacatt gatgtaaagg
     8341 tagatgctgt aaatatacta aataaaagta tagattttga atcttcagtt atctgttgtt
     8401 cttttaaaaa ctccttgtaa aaagcagata caagaataac ccttggaaat ggttgtggga
     8461 taaaaaatat taaaagtata cctaaaatat acgataaaag aatcattttt ttaggggtat
     8521 ttacagtatt taaaattaac ttctcagaaa taatttttgc gacacctgtt tttgtaactg
     8581 cattagtaat tatgtatgca agaattatta tgtaaaaatt ttctgtaaat aaaaactgta
     8641 aaataacctt tattggtgca atgttaaata taaacgctag aattacaaaa aacaatgcaa
     8701 caaaagtttt ttcaactgag tctacagccc atatcaagat agttaaaaaa gataagcata
     8761 tagaaaattt tatgttgaaa ctgtaattaa gagagaagta gagaattatg gtagctataa
     8821 cagtcatata aaattgtttt ggcttaagaa taatacccat ttttatattc acctccataa
     8881 tttattatat cataatggag gtgaatacaa tgaatgtgaa tacattttat aaaaagatta
     8941 ttcattgatt ctatgattta caatagcttt agttttccaa tgtcctttct tatagtagat
     9001 aactgtaaca ataagaccta aagtccaacc aaaaggaata cccatccaaa ttccgtctgt
     9061 tccaaaaaat ttagaaagca tataagaagc tggaagtctt ccaagccata aagatactat
     9121 tgagcttata agtgcaaaca tggaatcacc agcacctttc attgcacttg aaagtacaaa
     9181 agaagttcca ataaataaga agaatggtcc tattataaat aagtaagaag agccaatttt
     9241 aatgacatct ttatcagtat tgaatagagc aattagattt tccctaaata gcaaaattaa
     9301 agcaataact gtaactgcta aaactaaggt catttttata gattctcgaa tgcctttttt
     9361 aactctttca ttttctccag cacctatgtt ttgagcaaca aaagtagaaa cagcagaacc
     9421 aagattcata ataggtaagc ttataaaatt ttctatccta agaccagcac caaaagctgc
     9481 catagcagat gtaccataac tattaactaa aaattgaaga gccatatttc caattgaaaa
     9541 caacatttgt tgaactccac ttggaagtcc taatctaagt ccatctttaa atattttgtt
     9601 atcatataca attcccttga ttttaaattt aagcacttca tgttttttat ttaaataaat
     9661 tatacttatt ataaatgata ctgcctgagc cattatagtg gcaagtgcaa cgccctcaac
     9721 acccattctt agcacgacaa taaaagttaa gtctaaaact atatttagaa ttgtggctat
     9781 tatcagaaaa taaagtggag ttactgaatc tccaagtcct ctaagtattg cacttactga
     9841 attatatcca gcagaaaata atattccact aaatattata gtaagataca attttgattg
     9901 agcaaaaacg gattctggtg tattcataag cttaagcatg gggccaccaa atactattcc
     9961 aagaatactt attaatatag aaccaataaa taaaagtata tatgtagtat caatagtagc
    10021 ttttaccttt tttaaatctc ctgagccata aaattgtgac actaaaatag aaaatccaag
    10081 gctaacaccc ataagcgctg ccaccaataa aaacattata gggttagctc cagagactgc
    10141 cgccattgct tcttttccaa taaatctacc aactattata ctgtcagctg tattgtatag
    10201 ttgttgaaat aaactaccaa taagcatagg catggcaaaa aagaatatgg actttccctc
    10261 atgacctgta gttaaatctt tcaccttatt cctccaaata aaaaaattat taatttatta
    10321 tagtataatc ttaacataaa ctttatatcc aaactataaa taaattgtat caaaatatca
    10381 tatatttttt taaaatgta
//
LOCUS       DAENCV010000133        10266 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAENCV010000133 DAENCV010000000
VERSION     DAENCV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.135"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            398..1189
                     /locus_tag="KQD82_003173"
     CDS             398..1189
                     /locus_tag="KQD82_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG4381628.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            1218..2438
                     /locus_tag="KQD82_003174"
     CDS             1218..2438
                     /locus_tag="KQD82_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG4381629.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2458..3153
                     /gene="pxpB"
                     /locus_tag="KQD82_003175"
     CDS             2458..3153
                     /gene="pxpB"
                     /locus_tag="KQD82_003175"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG4381630.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3183..4169
                     /locus_tag="KQD82_003176"
     CDS             3183..4169
                     /locus_tag="KQD82_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG4381631.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4388..4954)
                     /locus_tag="KQD82_003177"
     CDS             complement(4388..4954)
                     /locus_tag="KQD82_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381632.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5306..6373
                     /locus_tag="KQD82_003178"
     CDS             5306..6373
                     /locus_tag="KQD82_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG4381633.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6392..7552
                     /locus_tag="KQD82_003179"
     CDS             6392..7552
                     /locus_tag="KQD82_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG4381634.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSNFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7565..9661
                     /locus_tag="KQD82_003180"
     CDS             7565..9661
                     /locus_tag="KQD82_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG4381635.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPLIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPFEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTVITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9820..10164)
                     /locus_tag="KQD82_003181"
     CDS             complement(9820..10164)
                     /locus_tag="KQD82_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG4381636.1"
                     /translation="MEIKRYEGTGRMSRTVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         3941 a         1168 c         1873 g         3284 t
ORIGIN      
        1 tataaagata ttttacttac aaagaatgat aattatatag atgaaagaaa caaatgaatg
       61 aagaaaaatg attctgtctt gcatataaag tgattaaaaa tgaaaaatac tttaaaaatg
      121 aaggttaagg cccttatttt aagtgaaaat aattaattat taactaaaaa atggtaaaaa
      181 gattttttga aaaagaaata caaatgaatg ttcaattaaa ataattgcaa aaatattcct
      241 taaatggtaa aattatatta attaaatgca atagaatagt tgcaaaaata aaattgaata
      301 caagtgataa aatatttcat gcaatatata aaataaacaa aatttaaaaa ttaaaactac
      361 ttattctatc aaaatgttag cgaggagggg tcataaattg tataaagtag atttaaatag
      421 tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag ttttaaaata
      481 tatttcatca gccaatatag cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa
      541 aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag gtttcctaga
      601 tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta aagattatac
      661 aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta atatacaaca
      721 tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt tagctatggg
      781 gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc tatataatag
      841 tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag tatttgcaga
      901 tagagcttat gataataatg gttttttggt tccaagaagt gtagaaggag cagttataca
      961 tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa ctgtagaaac
     1021 tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg gggacaatcc
     1081 aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaaa gtatagaagt
     1141 ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag actgataaca
     1201 tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa aataaaaaag
     1261 atgttggagc acttataggg gctgcattta taatggcaac atcagcaatt ggaccagggt
     1321 ttttaactca gactgcacaa tttacccaag attttggacc gaatttttca tttgtaattt
     1381 taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta attggtgtat
     1441 caggacttag aggtcaggat atagcaaata aaataatacc aggattaggt tatttagtag
     1501 cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt ggagctgcac
     1561 ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta agtggattaa
     1621 tagccatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag ataactaaat
     1681 ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat catcctcctg
     1741 ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggactg atattcccaa
     1801 taataactct acttggagga agtgttggag gttatataac ttttgctgga ggacatagat
     1861 taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa tcttctcttt
     1921 tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata ctggcagtgg
     1981 tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt aaatttagtg
     2041 caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca attacatcag
     2101 ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct tttatagata
     2161 aatatgaaaa atattttata atagcattta tagcaatatc aacacttata atggcattta
     2221 taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg atacttccaa
     2281 tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga gattacaagc
     2341 atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca tatacaggaa
     2401 taacttctct tagcagccta ggtgctttat ttgcttaact tagaagaggt gacatttatg
     2461 gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg aaatgagata
     2521 tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat atcgaatctt
     2581 aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag ttataatcca
     2641 ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga taatttggaa
     2701 agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga tgaggttttt
     2761 ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga agttataaaa
     2821 atacacacaa gtagagagta cttgatatat atgctagggt ttactcctgg atttccatat
     2881 ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag gactaaaatt
     2941 tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat tgatagtccg
     3001 ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa tagagaagaa
     3061 cagattttgc ttagagctgg agattttata aaatttgttc caataacatt agatgaattt
     3121 atagaaatcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag ggagtggtat
     3181 atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac agaggcagag
     3241 ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat gcacatagag
     3301 taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt ttaatgttag
     3361 gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga gatttagggg
     3421 ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat ccaggtgatg
     3481 tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata gctggtggaa
     3541 tagatgttcc acttgcaatg ggaagtaagt caacttatac aagagctaaa attggtggat
     3601 ttgaaggtag agcacttaaa aaaggagatt atataaatac ttttattcag gaaaaagatt
     3661 ttacaataaa taaaaaatta agttcaaaat atattccaac ttatagttca gaaatcgtac
     3721 taagaattgt aaaaggtcct caatttgatg ctttttctaa cggtgaagta gagaaatttt
     3781 tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga ctagatggag
     3841 agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct tatggagcta
     3901 tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag actagtggtg
     3961 gctatactaa gatagggaac gttataagtg tagatttgta taagttagca caagcaaagc
     4021 caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga ctattgagag
     4081 agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga gttgtaaagg
     4141 caaaagtatt aaatgatata gcagtttaat ttaattttaa caaatacaat ttttattata
     4201 atttataata taacttacaa atagctattt actatttacc ataattaaga tggtttagat
     4261 tagtcaaata gctatttttt tagtttgagt aaatatttta aatttaaata aatacttcaa
     4321 atttaaaata aaagcaatat ttaaatctat tatattatct acaaaaagta ggtgtgtcat
     4381 attgagttta tttagatttt attggccaaa ttggaggtga tataactcca tatccacgag
     4441 ctattgtatc tttacttgga ttgatgattt tatgtggtac attagctttt ataaatgttg
     4501 agtcacctgg atttaatcta actacctttt cacccataca aatatcaacc acaccactcg
     4561 ttaccaccat aatttcttct gatgcatgat atatataatc tttggcatcc tcactttctg
     4621 gtttaagttc aaactctaca aataaaattg aaggtgtaaa ctcagttgta ggcattggag
     4681 atactatctc gtaaaacatt tcactctttg gaaatttcat cataactcta tcttcttttc
     4741 taatgactaa atcatcagta ttattgtcat ccataaacat atataaagga gtgtctaaaa
     4801 cttcagcgat ttttctcaat gaagacaagg atggttcaat agcatctctt tctatttgag
     4861 agatataact tatagataaa tctgtcaatt tggaaacatc acttaatgtt tttttcttgc
     4921 tttttcttat atttctcatc ttttcaccta gcataagaac ctccaaataa taaaattaat
     4981 ataaaatatt cgcttaaaac aaagtaatta tagcacaaca tagaagtagt ggcaataaga
     5041 atacatagta gattttgaca attaaaattt aattaaatcc aaataaaagt ctatgaaaat
     5101 gaattattgt taaattaata caaagaaaat aataataaaa aaatttaata gaagagtatt
     5161 aattgaatta agattatcac attatatttt ggaaaagagt aaattttcag taaattatct
     5221 tgacatatat attgaagtaa taatataatt taaagtataa aattttaaaa attgaattat
     5281 aacttcaaaa aataaggagg gtaatatgaa gataacagat atcaaatttg aaaagcttag
     5341 aattaaatta aaaaaacctg tagtagtttc atttggagta attgaatatg gagaaagtgt
     5401 aattttaaaa attgaaactg atgaagggta ttgtggattt ggcgaagcag cacctcttgc
     5461 agcagttaca ggtgaagttt tggataatgt tctttcagta ttacttatgt ttaaaaaaga
     5521 gttaataggc aaagacccac tagatattga aaccatacat accatcatgg atggaattat
     5581 tataggtaat acttctgcaa aagcagctat tgacatagct ttatatgata ttaaaggaaa
     5641 aattatgaat gcaccccttt ataaagttct tggaggattt gatagtaaag tccaaacgga
     5701 tattactata agtatagata agccagaaaa aatggcagta gaagctttag aaagagtaaa
     5761 agaaggtttt aggatattga agcttaaagc aggtataaat cctgaagacg atattgaagc
     5821 tgtaaagtta attagagaag ccgttggaga gagtataaga attagaattg atgcaaatca
     5881 aggatggaac gtaaatagca gtataaatac tatcaaaaag ttagaagaat ttaatgttga
     5941 tgcaattgaa caagcacttc cacattggga tttagatggt actgcttaca taagaaataa
     6001 aagtaacaca aaaattatga tagatgaatc attacattca ccaatagatg caattaaggc
     6061 tataaaaaag aatgcagttg atacatttaa tattaagctg atgaaatcaa gtggaatata
     6121 tcctgctata aaaatcaaca acatagctga ggcaagtgga gtaaattgta tgttaggatg
     6181 tatgctggaa acgagaattg gtataacagc agcagcaaat ttaatagcat ctaaaaagaa
     6241 cataacagaa gctgaccttg atagttttat gttttgtgaa gaattagaag gtatatctgg
     6301 aggatttgta atggatggag atattatgaa tcttgtcaat aaaccaggtt taggaataga
     6361 agtaaactta tgataaattt ggaggttaaa tatgaataca cttttttcaa atccaggtag
     6421 tatacttgct gtaatgacat caatgattgc attgggtttt tacttacaaa gatacaaagc
     6481 cattaaaagc ttaggaccag cacttacaat aatcataatg ggaataatac tttcaaactt
     6541 aaaagtagtt ccagtaagta ctgagttata tggaactatt tcaacatatg ctattccagt
     6601 ttctatgact ataatgttga tgagtgttga cttaaaagaa atgacaaaat tatcaagaga
     6661 acctttaatt gcaatttttg tagctgtatt aactgtaagt atcatggcat ttttatttgg
     6721 attagtattt gcagaaaaaa tatcagaagg ttggaaggtt gctggaatgt ttgtaggtac
     6781 atacactggt ggaagtgcta atcttacagc tattggaaca ggtcttaatg taagtagaca
     6841 aactcttgca gcagcaaatg cagcagacta tgtaattgga gtaccaacat tgatatttat
     6901 gtttgctcta ccagcaatat taaaaaattc aaagaagttt aaaaaacttt ggccatatca
     6961 tgtagaagaa tcagaattag aagattgtca aaatgaagaa tttatggagt caaaagaatg
     7021 gagtattaaa gatatagctt ggatgttggc tataggtttt gttgttacag aagtttcaac
     7081 aatacttgct ggatatttca attcaagttt tagtagtgca gcaagaatac ttttagttac
     7141 aacaatttca attataattg ctcaattaaa gccagtaaag aaactaaagg gaaatttaga
     7201 tttaggatta tttgttgcat tattcttttt atgtacaata ggtttttcag tagatataaa
     7261 agagttttta ggttcaaatt ttactataac actttactgt tttagcataa tttttgcttc
     7321 atttgttttc catttaggca taactagact tttaaagata aagtatcaat atgtaatact
     7381 ttctatagta ggagcaattg ctgatggtcc tacttcagct ttagtagctg cttctgcaaa
     7441 gtggaattcg cttgtaagtg tagcagtcgt aatgggagta attggtggag tattaggaaa
     7501 ttatgcaggt atatcagtgg catatgcaat aaagatgtct ctaggattat aggggggagt
     7561 ttaattgata aaatttaaat tatacatatc tggactatac agtggaaatg taatctttga
     7621 tggagattta ttaattgaaa agttgaatcc ttttacaaat aaaatagaaa gtttaaaacc
     7681 aataagcaaa gaagaaaata catactattt aaatcttaca aaaatagatt taaagtcttt
     7741 atttaataac tttgatgtat atactaaaag cttagtaaac acagataaag acacagttat
     7801 aaataactta ggagaaacgc attctaaact taatgaatat atatgggttc aaagaaacaa
     7861 aaagttccca ctagatataa ttattgtaga taataaaata gtaggtttta tctgtttatc
     7921 cagagaaact tgtactatat taattatgga tggatatgaa gagtatactg tattaaaaga
     7981 atgggaaaaa actcataaaa atgaagagat atattctata agatttggtg gaaattatat
     8041 gattgatatg aaagatggta taaaactttc tacagatgta tatctccctg attttgtaga
     8101 ttctaccaaa aaagcaccga caattcttat gagaactcca tatggaaaag aaaatgataa
     8161 agaaatatat tataaatatg tacaaagagg atatgctgtt gtaatacaag atgttagggg
     8221 aagaaatgaa tcagaaggta agtgggaacc tttgattcat gagagggaag atggagatag
     8281 taccataaat tggatagtat cacaggaatg gtcaagtgga atagttggaa tgcttggagc
     8341 atcatatctt ggttatgtcc aatgggcagc tgcatcttca ggcaataagc atcttaaagc
     8401 tttagttagt atagtaacat caggaagccc ttttattgat ataccaagaa aaggaggagc
     8461 ttttgtatct ggtatgcttg catgggcatt tatggtatca agaaataaag ttgatagaag
     8521 caaaatggta agagatgatt gggatgatgt tttaaacata cgaccaatag aaaatatacc
     8581 ttttgaagct cttggatata gaattgaatt tctggaagaa tggttaaaga gagtagaaaa
     8641 agatgagtat tgggatttga tggattggca tttacaaaaa gataaaataa atgtacctgc
     8701 attggttgta tctgggtggt atgatgataa tagtatggga actacagagg ccttagatgt
     8761 tataaaggac tatgaaaaag gaaagagaaa ggctattcta ggtccatgga tgcataattc
     8821 aaatacttta agagatataa atggtatttc tttaggaaat agttcattaa gatatgattt
     8881 ggattataat tatctattat ggtttgataa atacttaaaa ggtatagaaa ataacataga
     8941 tacgactgct ccagtagagt attactctgt aggttctaat aagtggaaga cagaagaaaa
     9001 ttggccaata ataaataaga tagataaaag tatgtatcta ataagtgatg gaaatgctaa
     9061 cacatcttta ggaaatggta gattagtatt tgataatgat ttagaagaaa agtatgatag
     9121 ttatatatat aatccaaaag acccatcagt acaactaatt gatatgtcag agaatgaagt
     9181 tggagttcca aataactaca aagatttaga aaaaagaagt gatatgttgt gttatacatc
     9241 agatgctttt agtgaagaat ttactgtaac aggtgatata aagttagagt tttttgcatc
     9301 aagttctgcc aaagatactg actgggttat aaaaattatg gacgtggatt tagatggaaa
     9361 ttcaattaaa ttagctgatg gtatacttag tgctagattt agaaatagtt tttataagtc
     9421 agagtttatg gaagaaggag agatttataa gtttacggtt ataacttcaa aaatatcaaa
     9481 cacgtttaaa gttggtcata aaattagact agatataaca tctagtgcta aaaactttat
     9541 tttccaaaat agcaacacga cagagggata taatagtatt gaatatatag aagcaaagaa
     9601 tacgatttac catggaggta aatatccatc taaattgata ttgccaatag agaataagta
     9661 aatttatata gattaaatag tgaggaggtg tctcaaaatg aattttttag ttcgtgagac
     9721 actttctttt atacgaaatc aaatatattt tcatcatttc aacaatgaaa aagaggctta
     9781 tctctctgtt ttgagatagc ctcctataag ctaaattgat tattttgtag ctgcaacaac
     9841 gctcatttca actaacaaat gctctctagc aagtctagct tcaacacaag ctctagcagg
     9901 ttcaaaccct ttttcaaccc aagcatccca aacagagttc atagcttcaa agtctttcat
     9961 atctcttaaa taaatagtaa cagatagcaa atgttgctta tctgaaccat atttgtttaa
    10021 taaatcttct attttagcta aaacttcagt tgtttgttca attactcctc cttcagcatg
    10081 agtttgtcca cataaataaa ctgtattgtt atgaactaca gttctactca ttcttccagt
    10141 tccttcatat ctctttattt ccatatttta aatacctcca tagttaaaaa atctattaaa
    10201 aatctaaata ataaattaaa aaaagtctat actatataaa attatattat atattctgat
    10261 aataat
//
LOCUS       DAENCV010000134        10250 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAENCV010000134 DAENCV010000000
VERSION     DAENCV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.136"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(117..1871)
                     /locus_tag="KQD82_003182"
     CDS             complement(117..1871)
                     /locus_tag="KQD82_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG4381637.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(2089..2979)
                     /locus_tag="KQD82_003183"
     CDS             complement(2089..2979)
                     /locus_tag="KQD82_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4381638.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLIPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPPNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            3128..3988
                     /locus_tag="KQD82_003184"
     CDS             3128..3988
                     /locus_tag="KQD82_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG4381639.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4424..4990
                     /locus_tag="KQD82_003185"
     CDS             4424..4990
                     /locus_tag="KQD82_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4381640.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            5030..5611
                     /locus_tag="KQD82_003186"
     CDS             5030..5611
                     /locus_tag="KQD82_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4381641.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5668..6585)
                     /locus_tag="KQD82_003187"
     CDS             complement(5668..6585)
                     /locus_tag="KQD82_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4381642.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6715..8346
                     /locus_tag="KQD82_003188"
     CDS             6715..8346
                     /locus_tag="KQD82_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG4381643.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGTLFYPNLKVMEE"
     gene            8349..9296
                     /locus_tag="KQD82_003189"
     CDS             8349..9296
                     /locus_tag="KQD82_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG4381644.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(9581..9844)
                     /locus_tag="KQD82_003190"
     CDS             complement(9581..9844)
                     /locus_tag="KQD82_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG4381645.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(9943..10188)
                     /locus_tag="KQD82_003191"
     CDS             complement(9943..10188)
                     /locus_tag="KQD82_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381646.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         3585 a         1468 c         1426 g         3771 t
ORIGIN      
        1 ttattgataa atacttaatt atcacttaaa aaataatttt ttatttaaag aatatatata
       61 aatattttaa aattctataa tttttaaaac tatataaact ttattttcat atgtttttat
      121 tctccaattg atatattatt tatgccatta tttttaactt caataattgg ttgtccattt
      181 cctaaaatat aatttttaga ccaaccttct ggcaatttat ttatcccatt tccaattaac
      241 ttcaagtgta aatttgatgg ttcttttttc agatttaatt taaatattcc atcaaaggta
      301 cctttattat cgcttccaaa taaacttata ttgccattta tattcgtagc tgttacattt
      361 acaattccat tgtttgctct aaattttatt tcagatgata tagtttcagc tgttacacct
      421 gaaatcccac tattagtatt tatattgaaa ggatttgtta cattgttatc aacaatgtta
      481 gcaatcccgt cattgacatc aacagtagct cctcctttaa tattataaag ttctactgaa
      541 ccactttcaa catctacttt aaaattagaa tacattttat cgggaactcc aatttttatc
      601 acatttacca tcctatcttt atttgcattt atcatattgt cgccattcat atcttgcaaa
      661 tcactatctt tcttatctat ttttttctta gcacttacaa ctagctttcc attttttata
      721 ttattagaag aattttcttt atatacagaa aaagtacaat ttttttcatt agttacactt
      781 acgtaatcaa actcaaattt attggtattc gtcttttcaa caaccacacc taaattatct
      841 gattctataa gtatttcaga aacttcattt ccttcaactg aagttaagtg ttttaaaata
      901 tttggagtca tatccatatt attatattta atcatatcta aacactgttt atatatattg
      961 gcatcaatac ctccaacttt tgttatactt ttagctcctt ttaaaatacc tttattactt
     1021 ccatcagcaa ctaaaacgat tggtttttct ttggctaaag gagataatgt tagtgcatct
     1081 actaattgat atccatttgt tatataaaaa tctgtagctc cattataaaa cttttccatt
     1141 acctttttat tggtctcaaa tctatcattt cctccgattc taattgcttt agtttcattt
     1201 actaaatcct catttatcgc agatttgcca cctatagcgt aaacatttaa accttcagtc
     1261 ttaaatggaa ctgatttccc atctgtaagt attatagctc ctttattttt tgcaactaca
     1321 ggtgctatac tcatagcatc agcctctcct acaaatccat ttgtaagcat tacctctttt
     1381 acactactta cttcatttat ttttttagct acattataac tagtttcaat tctattgtta
     1441 ccatttattc tttccacttg aatacctttt ctttttatat ctttttctac tgaattttca
     1501 atagtattat aaccaccaat tatatatact ttttttacaa catttaaact cttattatct
     1561 agtctattta aagtagcttt tggtatttca ctttttttag tcaaaagtat tggtgcgtta
     1621 atagctccag ctaatccact tgatgaaagc ccatcagata aatttttatc tccattaact
     1681 aatattacag tatcataagt ttgtctatct gctataagtc ctgctgtttc atatctatca
     1741 attcctatta ttgaatcttc tttaatctgt gcatttgcag ttaaattatt aaaaagaaac
     1801 atagtcaaaa ctaaaattaa tatacttagt tttttactga attttttgaa ttttttattc
     1861 ataatataca ctattcctcc tgtaaattaa ggtctttaat ttgcttataa attatcccct
     1921 tatttacaaa taataacata atattatagt aacgtatata ttaccttaaa attatcagta
     1981 actttatatc aatcatctta gcttatttat ataaattttt ttatattact tttcaaataa
     2041 cagtcatact aaaacaattt catcatataa tttgaaaatt attactattt acacagcttt
     2101 tatcatcatc ttaccaccat caaatatata ttcaaccata ttattctcat ataaataagt
     2161 tatataagaa cgtattgtac ttccaaccaa cacatattga ttagcatcaa gcttgacatt
     2221 gtacaaatca cacatttttt caataacttc atcaatcata actggctcat aacaaacctt
     2281 tttaatatta tctattattt cttccatctt cttcttatta atattaatca aactttttat
     2341 atctgtagtt ttgacagagt gacttggaac aaagaaatca gcctctaaca ttctcaattt
     2401 ttctagtgtc ttaaactgtg aatctatatc cagtaggaag aaaaaatgat atttagtaat
     2461 tatattctct ggtgtaagac tatctgcaac aaacaaaaca ttatctggag ttctaacacc
     2521 aatcatttca aaataatgac ctggaagaga aatcgcttca agttctgtat ctacaatttt
     2581 gccatttgga ggtataatat aatcaacttc actttccttt gctaataaaa atttattctt
     2641 cagtgcttta cttggatagc caccatataa aaatgctgat tctaatattg gattttctgt
     2701 aaaatatcct tcaattttag tcgtagcaat cttgcatttt gtttgattct tcaaatatgc
     2761 atttcctcct atatggtctg catttgaatg agtgtttatt attaatttag gaattaaatt
     2821 atgttcttct aataatctta aaacttgtct agctgtatcc ttattatttc cactgtcaat
     2881 taaaattgca tctttaccat ctacgtatac acctactatt gtagggagtg gaatataata
     2941 tgtatttcct tttatatgtt ctagagttaa tttagccatt ttataacctc cctttaaatt
     3001 tacttttaat tatagcatta attttctgaa aattccaatc aaattgtttt tataatatat
     3061 ttttatttct tttattaaaa attggaaaaa ctatataata tatattataa tctatatata
     3121 aggagtgatg ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt
     3181 aggctgtata ttaatggcca tttctcttaa cttatttttt aatccacatg caatcgctgc
     3241 tggtggtata acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat
     3301 tgtcaattta cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa
     3361 agattgtttt aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc
     3421 aaatatggct acattagata taacaaatga tatgtatctt gcaattatat ctggttctat
     3481 tttaatggga gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga
     3541 tttaatggca ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg
     3601 tattgtagac tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc
     3661 actttattca actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg
     3721 atttaattat tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa
     3781 aataatggaa gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg
     3841 agaaaataaa aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattgaaaaa
     3901 attagtaaaa aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt
     3961 aggtaatgga tttaaaaaaa tagaataact aacaaataaa tagcaagtat aattaacata
     4021 tacaaattca actttttata tgttatatta tacttgctat ttatatatta taattgttgt
     4081 ttttaagata aaaattaaat gtatcaatta ttgttttata caagtatttc caaatccgtt
     4141 ctcatttaga tagtatgctt agaaatttta gaattattga gtctaacttt attacttact
     4201 ttactttata acaatcaaat acaaatttaa atattataaa actacaaaat aatttaaagc
     4261 atataatgtc aatatgttta aattataaaa tattcatgta gttatataat aaataattta
     4321 taaatatagc caacttagcc taaatacgta tataatatag ctaatatggt tcttatactt
     4381 gataccatca ataaaactaa aaatataaaa aggagaactt aaaatgagtg ttagtttaca
     4441 aaaaggacaa agagttagct tagtaaaaga taataatcct gtaaaaaatc tagtagtagg
     4501 cttaggatgg gatatgaata aattgggtaa aaaaaattat gacctagatg catttgcaat
     4561 agcattgact aaccaagata aaatgaatgt tagaggtgac ttggtttatt ttggaaatct
     4621 taaacaccct tctaaagcta ttatacacac tggagataac ttaactggaa aaggtgaagg
     4681 tgatgatgaa caaataattg ttaatttaga agacatacct gaatatgtac ataaaatagt
     4741 ttttggagtt tctatataca aagctaaaaa aagagaccag gattttggtc aaatgaacaa
     4801 ctcttttata agattaattg attcaaattc caagcaagaa ttatttaaat ataatcttca
     4861 agaagacttt tctggtaaag taacagtttt ggcaggagaa atttatcgta gaaatgaaga
     4921 atggaaattt aatgctttag gaattggtca aaatgaagag cttcgtgaat taattaatac
     4981 atataagtaa taattacaat aaaaaattta taatgaggag ttggtttaaa tgggtataac
     5041 tttagcaaaa ggacaaaaag taagtttaac aaaaagtaac cctggtctaa aaaaagtaat
     5101 agtaggacta ggatgggata ttaataaata tgatggtggc tttgatttcg atttagatgc
     5161 ctctgcattt ttaactggaa ctgatggaaa agtaacgaat gatggagact ttatattcta
     5221 taataattta aaacacgctt ctggagctgt tgaatatatg ggtgataata gaacaggtgt
     5281 tggtgatggt gatgatgaac aaataaatgt agacttatct aaaatacctc aaaatattgc
     5341 taaaatatca tttagtgtta ctataaacga agcaattaca agaagacaaa atttcggaca
     5401 agttgaaaat tcatatataa gaatatataa tgaagaaact aatgaggaac ttattaaata
     5461 tgaattgggt gaagatttta gcatagaaac agcaatagtt gtggcagaac tatatagaca
     5521 taatggagag tggaaattta atgctttagg ttctggattt gaggatggac ttgcaggatt
     5581 gtgtaaaaat tttggcgtta atataggcta atatatctaa ataaaaataa gagtatattt
     5641 atatactctt atttttattt agatatatta taaactgttt acacttctac tcttctttct
     5701 cgcttgtttt ttcccatatt ctactgaaat atcaaaaaat gctgaaatat tttctgtaaa
     5761 aaacttgctt ctatgatatg caatatcaaa tgtcctatgt aaattaatac cttcaatatc
     5821 acaagcccac aaaagatgct tttttaaata atcctcaata agtaattcgg atataactgc
     5881 aatgccaaaa ttatctacaa ctgctcttaa tatagcttca gagttataac atgaccatct
     5941 aatattcatt gggattttta attcttttaa ttgcttttct aattgtgctc ttgtaccact
     6001 tcctaattca cgcataatta atggttgatt tgaaagttct gaaactttta tagagtctct
     6061 tttgtagaac tcatgcttgt gtgaacatat aagtacaaga ttatcattaa taatgctttt
     6121 tgtaacaata tctgggtgct taatttttcc ttctacaatc gcaatatcta attcactatt
     6181 aagtaatttt tttgcaatca gtcttgtatc ttctacaaat acttcaggtt ctataagagg
     6241 atttaccttg tataattcta acattattgg acttattaca catgtaccaa cagttactgt
     6301 tgccccaact cttatacgga tattttttgc acaatgcttc atacaatcat tcatttcttc
     6361 atttacagct aaaagatgcc tcccatatcc aagaagtttt ttccctgcct cagttaaata
     6421 taattttttt gaaattctat caaatagctt aacaccatac tctttttcta tttctgaaat
     6481 agcttggcta actgatgact gggtaatata catatttctt gctacttcac tcattttacc
     6541 acattcagca accttaacaa agatttcaag actacgaatt gtcatattat cacaacctta
     6601 gcaaacatta gtaaatgctt atgattttat taatatttta gtattttaca aatatattgt
     6661 caagaagtat aattttatta tagagttata aagtagattg gagtgtgata tattgtgaaa
     6721 gtattaatta taggtggagt tgctgctgga acaaaaactg ctgcaaaaat aaaacgtgaa
     6781 atgggtgata attgctctgt tacaatacta aataaaggtg atgatatttc ttatgcaggt
     6841 tgtggtcttc catattacgt tggtaagata attgaagata aatcatcttt aatcgtaaat
     6901 acacctgaaa gtttttctaa actaacagga gcagaagttc gttgtggagt tgaagttaca
     6961 tctattgata gaaatgcaaa acaagtaact ttgaaaacgc tttcaacagg agaaaaagaa
     7021 aatctaagct atgacaaact tgtaattgct acaggggcag acccaataaa accaccaata
     7081 cctggaatag atttacctgg agtattcttt atgcgtactc caaatgatgc tataaaacta
     7141 agagattcta ttgaaaacgg tattaagaga gctgttgtta ttggtggagg tttcattggt
     7201 cttgaaattg ctgaaaatct ttctgcaatg ggtatacgtg taagtgttgt tgatatggct
     7261 gaacatgtta tgcctggatt tgatactgat tttgctgaat acgttgaaaa tcatatggct
     7321 gataaaggca taatgatttt tacaggtgac caggttgtag gtattgaagg tgaaaataaa
     7381 gttgaaaaac ttcgtactaa aaatagagct attaaaacag atttagtggt tatgtcagtt
     7441 ggaatccgtc ctaacacagg ttttctaaaa gacacaggtt tagaatttgc ccctaataat
     7501 acaattattg cagatgaata tatgcttaca aatgacaaag atatatacgt agttggagac
     7561 tgtgcttttg taaaaaattc tttaacaaat aaacctacat ggtcacctat ggggtcatct
     7621 gcaaatcatg agggtagaat ttgcgcacaa aatatatctg gaaaatctaa aacttacaat
     7681 ggtgttcttg gaacaacagt agtaaaatta cctgagctca atgcaggaaa aacaggtcta
     7741 agtaaagaaa tagctgaaaa agaaggttat aatgtttgct ctgtaaccat agctactgat
     7801 gataaagctc attattatcc aggtgcatct aattttatta taagaatggt tgctgaaaaa
     7861 aatactagaa aattattagg tgttcaagtt atgggacctg gagcggttga taaaatagta
     7921 gatatcgctg ctactgcaat aactttaggc gctgaccttt attcattaga aagtatggac
     7981 cttgcatatg caccaccttt ttcaacagct attcaccctt ttgtagtggc agtaaatgtt
     8041 ttacaaaata aacttaatgg agaacttgat agtgttttac ttgacgaaat tgaggatttt
     8101 gacagttgga ctaaacttga tgtttcaaaa gctccttcta ttcctagttt acgttatatt
     8161 cctgttggtg aaattaatgg agaaattgaa ggccttgcta aggatgaaaa aattcttctt
     8221 atatgtgcta aaggtaaaaa tagctatatg gcgcaaaatc gcttaagacg ctttggctat
     8281 acaaatacga aagttcttga aggtggaaca ctattttatc caaatcttaa ggtaatggag
     8341 gagtaaatat gagtaatgtt acagcagaac aaaaaaagga agtaaaaggt agaggttttc
     8401 taccaagcag ggatggagaa cattttgcag cacgtataat aactgtaaat ggtgtaataa
     8461 atgcatcaca agcaaaaaaa attgcagaag ctgctgaaaa attcggaaac ggtcagttag
     8521 catttactac tagactaaca gtagaattac ctggtgtaaa atttgaagat attgaagctc
     8581 taagtgaatt tattgctagc gaaaatctaa taacaggtgg aactggatca agggttcgtc
     8641 ctgttgtggc ttgtaaaggt actgtttgtg ttcatggtct agctgataca caggcacttg
     8701 ctgctgaaat acacgaagaa ttttacaaaa attggtatga tgtaaaacta cctcataagt
     8761 tcaaaattgg tgttggtgga tgccctaata actgtattaa acctgaccta aatgatcttg
     8821 gaattgttgg tcaacgtgta cctgactatg attcagagct ttgtgttggt tgtaaaaaat
     8881 gtgctgttgt agaggtctgt cctgttaaag cagctaaatt aacagataaa ggtaaacttg
     8941 aaatagatag taatctatgt aataactgtg gtaagtgtat agaaagttgt aattttgata
     9001 gtattgaaga aaaagaatct ggttataaag tctatatagg agggaaatgg ggaaaatcag
     9061 ttcgtcctgg aacacagatt gatagattat tttcaaaaga ggaacttatg actttaattg
     9121 aaaaagcaat tcttttattt agagaacagg gtaacacagg tgaacgtttt ggaataacta
     9181 tcgatagaat cggtgctgat aaatttatag aaatgctttt atcagatgaa gttttggaaa
     9241 gaaaagaaga gatactagag gctcttttac accttacagg tggagctgta tgctaacaat
     9301 aaatatttta attgtacaga taacctatgg aaaaaatttt agttaagaat aattaaaatt
     9361 ctatatatga tatatgataa atgaggctat ctcaaaacac agatacgcct ctttttcatt
     9421 gttgaaatga taaagtatat ttgagttcat ataaaaaaga gtgtctcgtg aattataaaa
     9481 ttcattttga ggcacccaca ttattttata ataattgctt attaatttta ttttaactat
     9541 ataaaaagta caaaaagtaa gaatatccaa tattctaaaa ttaattttga attgtattga
     9601 ttgaaaagtt tactattcca ctatttccat cttgatttac tactagatac catgttccag
     9661 tattaggaac tctcattcta catggatttt tctttgcaaa tccaccatag taatcataat
     9721 ttctttgatt tttaaatttc acataattta tatggtctaa caatattaca ttggcttgtt
     9781 tctctaaatt tacctctaca atatcccctt tttttaagtt catcttacta taactatatt
     9841 ccattgaatt actcctccag tatcgaataa tctttgttat ttactcatct caaatatgta
     9901 aaaattcatt taaaatatta caaatactat ataattagac tattatccag ttttaacctt
     9961 atttactaca atccaaaggt tattaaaaat attattggca acaacttctc catcccatac
    10021 aactctttct tctttatttt ctaataaagc atagtaccca gagtcagaac tttcatcttt
    10081 atagactgta acataaaact cttcatcact atattcaggt atatttttaa agtcttcaga
    10141 tattttacat gttaatatat aaccttctaa ttcttcatct ataaacaaag caattcctcc
    10201 aatctctata tttttactaa ttataccata ttatgtcata aactttttat
//
LOCUS       DAENCV010000135        10235 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAENCV010000135 DAENCV010000000
VERSION     DAENCV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.137"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..1429
                     /locus_tag="KQD82_003192"
     CDS             <1..1429
                     /locus_tag="KQD82_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4381647.1"
                     /translation="LDTGIKDKYKELFLKIEELDTILNLSKDGILFTSKDGEINTYNS
                     KVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIYNRDE
                     KMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSK
                     SDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTF
                     TGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDV
                     RIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLII
                     TPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKY
                     SKNDILNILEILILNKESDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEELIIC
                     SSGRYGSKITQKGEDFYNKLKYKGL"
     gene            1798..3018
                     /locus_tag="KQD82_003193"
     CDS             1798..3018
                     /locus_tag="KQD82_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381648.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDTHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3055..4404
                     /locus_tag="KQD82_003194"
     CDS             3055..4404
                     /locus_tag="KQD82_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4381649.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSSWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            4518..5080
                     /locus_tag="KQD82_003195"
                     /pseudo
     CDS             4518..5080
                     /locus_tag="KQD82_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5188..7521)
                     /locus_tag="KQD82_003196"
     CDS             complement(5188..7521)
                     /locus_tag="KQD82_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4381650.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEATHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(7518..8201)
                     /locus_tag="KQD82_003197"
     CDS             complement(7518..8201)
                     /locus_tag="KQD82_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381651.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(8272..9513)
                     /locus_tag="KQD82_003198"
     CDS             complement(8272..9513)
                     /locus_tag="KQD82_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381652.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(9524..10210)
                     /locus_tag="KQD82_003199"
     CDS             complement(9524..10210)
                     /locus_tag="KQD82_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381653.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
BASE COUNT         3736 a         1492 c         1339 g         3668 t
ORIGIN      
        1 tttagatact ggtataaaag ataaatataa ggaattattt ttaaaaatag aagaattaga
       61 tacaatattg aatttatcaa aagatggtat attatttact tcaaaagatg gtgaaataaa
      121 tacatataat agtaaagtaa aagatatttt agacataaat gaggatatat atggaaaata
      181 cattgaggat atatttgtag atagtttaaa agttttattg agtgagaaag agatactaga
      241 taaagttgtc gtattcaata agaaatatat aaatgtaaat aaaaaaaaca tatacaatag
      301 agatgaaaaa atgggcacat attatagttt acaagaaata acatacataa aaaaattaga
      361 acaaaatctc actaaaaaat taagagaaaa aggacaaata gcaaaatata catttaagga
      421 tataaaaaca aatagtccta aaatgtttga atgtatagat ttagctaaga aggtttctaa
      481 atctgattta agcatactta tcagaggtga aagtggaact ggtaaagaac tcatagctca
      541 gtctatacat aacaactcaa atagaaaaaa tcaacccttt atagcagtaa attgtgcagc
      601 cgttcctgaa aatttattag aaagccagct attcggatat gacaagggaa cttttactgg
      661 aggtctaaaa gatggaaaac aaggattatt tgaacttgct aataatggaa caatattctt
      721 agatgaaata ggcgatatgc ctttagaatt acaaacaaaa ctacttagag ttcttcaaga
      781 aaagcagata atgcctgttg ggtctcacaa tgtaattaat atagatgtta gaatcatatc
      841 tgctacaaat aagaatttag aacaaatgat tgataatagc caatttagag aggatttata
      901 ttatcgatta aatacaattc caataaatat acctcctcta agagaaagaa aagaagacat
      961 attaattatt atggaagatt taattaataa gaaattaata atcacaccag aagcaaaaaa
     1021 acttatacaa aactatatgt ggaaaggtaa tataagagaa ctccaaaatg ttacatccta
     1081 tttaaacatt atgtgtgaag atattgtatt agagaaagat ttacctccaa acttaaggtc
     1141 ttcagataac aaaaatacat cattaaaact aaaatatagt aagaatgata tattgaatat
     1201 attagagata ctaattttaa ataaggaatc agatgttggt attggtagag gattaatttt
     1261 aaaagcacta ctcgataaga accttcaaat tacagaagga aaaattaaaa aaatatttga
     1321 atatctaaaa aaagaagaac ttataatttg tagttcagga agatatggaa gtaaaataac
     1381 tcaaaaagga gaagattttt acaataaatt aaaatataaa ggtctataaa attataattt
     1441 atcaaacaaa gaaatctatt caggattaaa gtactccttc aataggtttt tttatttttc
     1501 aagtacccta aaattataat ttattttgta cataaataat aggaattaat aggaatagga
     1561 taattttttt atttcctatt ttttttatat aaactagcat ataaaatata ttttcttcaa
     1621 aataatattt tttactgaaa attgaaacta tgcaatcatt ttttatacta tacatataga
     1681 taaatgatta tatttattca atattcttat aaaaaacaaa attctccatc gaaaattatg
     1741 gcataataat tgcttattat attaataaaa caataaaaag caattggagg aataaaaatg
     1801 caacaggctt tgaaattaaa atatcaaacc aaagaattag gaaaagattt tgtaatagaa
     1861 agttgtaatt ctataaaacc atggcttata aacataagaa gagaattaca taaaattcca
     1921 gaattagctc ttgaagaaaa tcttactaaa cagaaggtaa tttcttattt aaaagaaatc
     1981 ggaattgatt atatggaatt tacaaaacac aatggaatta tggcatatat tttaaaagaa
     2041 agtgcagata aaacgatttg tataagagca gatatggatg ctcttcctat tgaagaagaa
     2101 aacaacatac cttataaatc aatacactct ggaaaaatgc atgcttgtgg tcacgatgca
     2161 catacaacca tgctacttgg agcatgtaaa gttttacatt caataaaaga taaacttaat
     2221 gtaaatgtaa aattcttatt tcaaccagca gaagaaggtt ttggaggagc aaaattttta
     2281 gttgaggatg ggtgtttaga aaatcccaag gccgattata tattcggact tcatgttatg
     2341 ccacatatag aaacaggcct tatcgaaaca aaatatgata cattaaatgc tagtgtagac
     2401 actataaaaa taagtatcaa aggtaaaaga gctcatggtg catatcctga aaacggtatt
     2461 gatgcaatag ttacagcttc acaaattgtt acatcattac aaactataat aagtagaaac
     2521 ttagaaccaa ataatgctgc tgttttgact ataggtaaaa tatatggtgg tgatacacac
     2581 aatgtaatat gtgaagatgt aaaattagaa ggcactttaa gaaccttaaa tagcaaaaca
     2641 agaaacttta tgattgataa aatagctaaa attgttggag acactgcatc tgcatttggt
     2701 tgtgttggga ctttacatgt aagtgatgaa aactatcctg ctgttataaa tgaaaaagaa
     2761 ttagtagata cagttatatc aaatactaaa gaattacttg gagaggaaaa atttatattg
     2821 agaccaaacc cgtctttagg tggtgaagac ttttcttttt acacagaaca ttgtaaagga
     2881 gcattcttcc atttaggatg taaaaatgag gaaaagggct taatttcacc tcttcatacc
     2941 tctagcttta atatagatga agattgttta ccaataggtg ttatgatgca tgtaatgaat
     3001 actttgtatt ttaattagta cagtaattaa taaaagaata ggagataaat taatatggaa
     3061 aaagctaaga aattaggtct atttagtatg atattattag ggattaatag cataataggt
     3121 tcaggtatat tcttactacc aggtaaagtt tataatctag caggtcaaaa cagtatgttt
     3181 atatacattt ttgctacact cttagttcta tcaatacttt tatgttttgc agaagttgga
     3241 agcatgtttg ataagaatgg aggagcatat ctatactcca aaaaagcttt tggagatttt
     3301 ataggatttg aagttggtac aatgtcttgg gttattagaa ttatatcatg gtctactcta
     3361 gcagtaggat ttgcaacagc tttaggttca ttttggccag aatctgccac tgaatacaaa
     3421 ggttatatag ctgcaatact cgtaacacta ctttcaataa atagcttatt tggaattaaa
     3481 agcacaaaga taatgaataa tgtaataact atagctaaat tagtaccatt aatagttttt
     3541 ataatagttg gaatattctt tataaaattt gtaaatattg ttccttcagg aaatgtagtt
     3601 aactctagta tgggtccagc cattatatta gtattttacg catttaccgg ctttgaatca
     3661 tttattgtgg caagtggaga aatggaaaat cctaaaaaaa atcttccagt cgcattaata
     3721 accacaatat ttatatgtgc aataatttat atattaattc aaattgtctg tatgggtata
     3781 cttggagata gactatttga aaacagtatt ccaattgcag atgcttcaag tgtattttta
     3841 ggcaattatg gtaaagtttt catatctgta gcaacattaa tatctatatt tggtataaat
     3901 ataggttcat caatagtaac tcctaaatgt ggttcttctc tagcagaaga aggttctctt
     3961 cctgcattta taggaaaaac aaataaatat ggtgctccat atgtagcaat aataatatct
     4021 ttaatttgtt gtataccact tgtgctaact ggaagttttg aacaacttgc agtaatgagt
     4081 gtaattgcaa gatttgctca gtacatacca acttgtttat cagtaatagt tttaagaaaa
     4141 agaacagatg ttaaggcctc ttttaagatt ccatttggtc ctgttatacc aatggtagct
     4201 attttaggta gctcatggtt gttacaacaa gcttgggcag aagatatttc aaaacctata
     4261 actcaaaata gagttttaat aggattaggg gcaatgttgt taatagctcc tctatatata
     4321 tttatgaaaa aaaataagga aatcgagcat aaaaataaat cagaagattc taaattatat
     4381 atttcgagag aaaaaattca ataatatttt acaaactatt taaaaattat ttttctaata
     4441 ctatagaaat aattagaaat ggagatgtta cgtatctaaa agtagttgat aacacggata
     4501 ttggtacttt tatagatatg ggacttacaa aagacatact agttcctttt aaagctaaga
     4561 cttatccatt gtttagagat gaaaaatatt tattctatat atacttggat aaaagcggaa
     4621 gaatagctgc tactacagac atagattcat atttagaaac agaccatact tacaatgtag
     4681 gagatgttgt aaatggagtt gtatatggat tccaaaccaa caactctgct atgatatgtg
     4741 ttgacaataa atatgctggt gtaatccttc ataatgaata ctttacagaa ttaaaagctg
     4801 gggatgttct tgaaaattta catgttataa aaatatacga tgatggtaaa ttaggacttt
     4861 ctcctagagg aaacagaaaa gatgaacttg ataccttaga aaataagatt ttaagctatt
     4921 tagaaggttc agatggatat atgagattca atgataaatc tgatcctaaa gatatttcta
     4981 tcttatttaa ttcaagtaag aaaattttaa acgtgctctt ggagtactta tgaagaaaga
     5041 cttaatttat caagatgaag atggaactta tctaaagtaa attatataac acaaaaatcg
     5101 aagtaaatta tgaaagatat tttctatttc atatttactt cgatttttta tttaaattac
     5161 tacctctatt taaattacta cttatattta ttgtgcattt actacctcta ttatgtctaa
     5221 ttcacaaatc tttttaatgg gttttcttac tgcaagcaaa gttattatta tcatagttac
     5281 tacaatgata aacagtaaat caaaaggtat ttgccagtct atatggaatt ttaatgttac
     5341 taaggataca aatatatatc tatgtaatat taatccaagt acactcccca ataaacagcc
     5401 acttacagca tatgtacttg attctactat taccattttt cttaattgct tattggacat
     5461 gcctattgca cgcataatac catagtaatt tattctactt gtaacgctta tattcatact
     5521 gtttatgata ttaaacatac ttataatcgc aattataaat acaaacccat atacaaaaac
     5581 tgccatagtg taaaatgaat ttttagcctc taagttacct tgtcgcttat ctcgaacctc
     5641 taaattatca tctagaacat ttcttatatt ttggattaaa tcttcaggag catcttcatc
     5701 aacttgagta tcaattatta tatagtcttt tatatctgtt atactagtaa aagtattttc
     5761 agatgttata atatttccaa tatatttgtc tttaacatca aatgaaattg aagaaagtat
     5821 acctgctacc tctatctctt tcttagaatt tccattttta atatatatct tatctccaat
     5881 attaaaccca tgaccttctt ctaccaatcc gatattttct gactctaatt tatcttcatt
     5941 gatttcccct tttactagat actttttggc ccagtcaaat tgattttttt cataagaaat
     6001 tagctgagat gttgcctcct tattattgta tgtagcctct acatccgtca tcattctact
     6061 gtatactttt tttattcctt tgatattact tatttcatta atatcgtctt tagtaagaac
     6121 agacttaaag tcagcgtcaa caatagatat atgagtagct tctggtttta taggtttaag
     6181 tgcctgatac ataaagtcta taaatacact aaaacctaaa aacaatacaa tactaatagc
     6241 aaatgaagaa gtcataagta taaaagattt ttttcttgaa aaggcatgat ttattcccat
     6301 agaaacatca attttagaac ttcctgcgtt agcaatttta cttgcttttc cactagttaa
     6361 aatttctttg aacattagat taccacgtat agcacttagt ggagacaccc cagaagcttt
     6421 ttttgctgga acaatagctg ataatattac tgttaaaaat cctactatta ttccaaacac
     6481 aataccaata aagctaacct ttaaaattgg catcgaacca aataactctg tattcacata
     6541 tttgagaaat atagaggata taatggttac aatacatcct aataacaatc ctagtggaat
     6601 agcctttaca cttagaatta aactttctaa aacaacaaat tttttgactt ggagttttga
     6661 tgctcccaag caacgtaata gtccaaaaaa ctttattctc tctattacac ccatattaaa
     6721 gctattatat atcatgataa tacttgctat taacacaagt ataaaaagtc ctcctgctgt
     6781 taagtaaagt ggtttagaat aatcagagtc accttgacct ataatagaaa gtaatttttc
     6841 atttttaccg atttgtttat cacttaaatt aaaggtattc ttaatatcag aagtagcttt
     6901 tctaatatta accttgtctt taaatgtaat ataataataa tcatatgttt ctgatttttt
     6961 gatagcttgt gtacctaata ttgaaagcat taatccatgc ttatctgagg ctcttaagct
     7021 tgataatgat gaatattcac ctactatctt ataattttta tttctaccat ctgctaattt
     7081 tattttgata aaatctccta cttttagatt aaagctttta actgcatcta catctattaa
     7141 agtctcattc tccacaacag gataacgacc ttttattatt tctagaccta attgttttgc
     7201 taaatcttca tctaaacctt gtatcaaaat ctcctttgat ttaaactctc cattatcaat
     7261 gacttgtcct ctaccatagt gagctatatc tactctacta cttatttctg atatagcttc
     7321 ttcattaaga tttttaagta ctatgtgcca atttccatca tcttttataa attgtacttt
     7381 ttgacattgt atagccatgt ctgctagtcc aaaaatagca gtaactaaac atactgcaat
     7441 gacaatacat aaaattgtag ttctattttt tttcttatgt acctttgcat attgtggtac
     7501 tagttctaaa tagcttctca ttctctattc acccctaggt ctttaagcat tccatcagaa
     7561 acctgtagta tcctatctgc tgtagaagct atatttgtat tatgagttat cataataata
     7621 gtttggttat atttttctgc tgtcactttt aaaagtgaaa ttacatcact gctattttta
     7681 ctgtcaagat ttcctgttgg ctcatctgcc aatataattg atggctttgt aatcaatgct
     7741 cttccaattg caaccctttg ttgctgacca ccagataatt gatttggaag atgacttttt
     7801 ctatctttaa gacctaaaat tgtcaatact tcatctagat aaatagtgtc tggttttttg
     7861 taatctagta gtaaaggaaa tattatattt tgttccacag ttaattcggg tataagatta
     7921 tatgattgaa agataaaacc tatattacga cgtcgaaaaa tagtctgttt ttcttctttc
     7981 attgaacata tttctttacc atcaacaaat atttttcctg aggttggagt atcaagacct
     8041 cctaaaagat ttaataatgt actcttacct gagccagatt ctccaactac agtaacaaac
     8101 tctccctttg aaactgtaaa tgatacatct tttaatgcct ctacttttgt atatccattt
     8161 ccatatgttt tacataaatt attaaccaca agaatatcca tttgctctat ctcctttctg
     8221 atgattaata taagaataat tcatacacct tactgtaaga tgtatcctat cttacatatt
     8281 agtaagcttt aaaaaactta gagtaaatgt acttccttgt aaaaaattac tttctacagt
     8341 tatggttcca ttgtgctgtt ctatgataga tttaactagt ggcaacccta atccaaggcc
     8401 ttgattactt tttgaaaact tactcctata aaatctttta aatatatgat gtatatcttc
     8461 aatatgaatt ccatttccgg tatcactcac tgaaataata gttgttatag gtgtttcaac
     8521 ccattctatc attattttat cgttctcttt tgtattatct aatgcatttt ttataaggtt
     8581 gcttaaagct tctgatatcc attcataatc gcaaaacaag tttgtatttt ctagacctct
     8641 tatagagata ctctttcctt ctttttctgc tctagtttca aaactatata aaattttagt
     8701 aatcaacttt tttacgttaa cactagcttt gttaagaatt attgtctttg aatctatctt
     8761 tgtaatcttt aacaagtttt gtatcaatga ttctatacgc tctaattcat tttgactctt
     8821 ctgcacaaaa ttctcaacaa tttcactatt atgactctct tcctgcaata tttgattata
     8881 catcatcaat gctgtaagtg gagtttttag ttgatgtgat atatctgaaa ttgtattttt
     8941 aagaaattct ttattctgct tttctttcaa tatatgtgtg tttagagatg ttgacatagc
     9001 atctattgaa ccaaataact ttgataaact accttcttca taagtattta accgtacatt
     9061 tatatttcca ttcataaatg aatcaatttt ataactagct tcttctatct tgttctgttg
     9121 cctaaaaaaa tacacataca atataaaaat aatcatagta aagaacacaa ataaatacaa
     9181 aaacatagtt tttatatatt taccttgaaa caatttcaca cttggcataa aactatcatc
     9241 tgtaccaaag tcatatctag ctgtatttaa gatttctttt ccttttaaag tatatttatc
     9301 ttcattagta gtaaatactg ctggtatttt tgatttatca acacccatac tatttaagta
     9361 tccagctata ttataatcat gttctaacag tgcactttta taatcattag ttatatttat
     9421 aactactatt tgacctacta ctataaacat caataaaacg gatataatca ccaaaaaaaa
     9481 tatttttatg tctttattag aaaatacctt catattaaca ccctcacttt ataacattcc
     9541 atttgtaacc cattcttcta actgttaata gattttttgg attttcagga ttatcttcaa
     9601 tcttatttct taaccttctt acatatactg acagagtatt atcatctaca aaactaccat
     9661 ttccatccca taacttatcc aatatatttt ttcttgtaag tacaatattt ttatttttca
     9721 taagtaagca taataattta tattctgctg tagtaagctc aagctcaatt tcatttttaa
     9781 aaacacgatt ttcaagtaat ttaacagtaa tattatttga ttttagttca gttacatttt
     9841 cacttgtaaa attataacgt ctaagtaaag cttttattct tgaaattaac tcatttaatt
     9901 taaaaggttt tgttatgtaa tcatctccac ccatatcaag tcccatcact acatttactt
     9961 cttcatcaga agcagttaaa aatatgattg gtacattaga agcttctctt acccttttac
    10021 aaatatcaaa accatcacca tcaggtaagg atacatccaa aagcagtaaa tcataatttt
    10081 tttctttata aatttgcaat gcttcttcta ctgttcttgc tacatcaaca ctaaatccat
    10141 tcttttgtat agaatattct aatccatata tcaaacttaa atcatcttca accaacaata
    10201 tactactcat ctcaatcact tccatttttt tattt
//
LOCUS       DAENCV010000136        10164 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAENCV010000136 DAENCV010000000
VERSION     DAENCV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.138"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..865
                     /locus_tag="KQD82_003200"
     CDS             <1..865
                     /locus_tag="KQD82_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4381654.1"
                     /translation="YKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIEN
                     ENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGH
                     SLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSL
                     FKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIR
                     IFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRAS
                     FPKFSGGDFFE"
     gene            858..1505
                     /locus_tag="KQD82_003201"
     CDS             858..1505
                     /locus_tag="KQD82_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381655.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1634..2569
                     /locus_tag="KQD82_003202"
     CDS             1634..2569
                     /locus_tag="KQD82_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381656.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2583..3680
                     /locus_tag="KQD82_003203"
     CDS             2583..3680
                     /locus_tag="KQD82_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381657.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3683..4747
                     /locus_tag="KQD82_003204"
     CDS             3683..4747
                     /locus_tag="KQD82_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381658.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVFDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIDLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4991..5488
                     /locus_tag="KQD82_003205"
     CDS             4991..5488
                     /locus_tag="KQD82_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG4381659.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5664..5912)
                     /locus_tag="KQD82_003206"
     CDS             complement(5664..5912)
                     /locus_tag="KQD82_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381660.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5978..6994)
                     /locus_tag="KQD82_003207"
     CDS             complement(5978..6994)
                     /locus_tag="KQD82_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG4381661.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7052..7819)
                     /locus_tag="KQD82_003208"
     CDS             complement(7052..7819)
                     /locus_tag="KQD82_003208"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG4381662.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7887..10076)
                     /locus_tag="KQD82_003209"
     CDS             complement(7887..10076)
                     /locus_tag="KQD82_003209"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG4381663.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3527 a         1567 c         1186 g         3884 t
ORIGIN      
        1 atataagtac atattgttct cttttgttat aatatcttct cttattatta gtttagataa
       61 aaatatatat ttggcactgg agtcaagctc tatattgcta attataactg tactttctat
      121 atatatagaa aatgagaatt tatcaaaatt atacagtcaa aatctttatg ataagcttag
      181 aatttctaaa gatgaattaa aaaaagttaa tgctgatttg gaaacttatg caagttctat
      241 agaagaatta gcaattttaa aagaaagaaa tcgaatatca agagaaatac atgatagtgt
      301 aggacattct ctttctacaa caataataca attaaatgct atagaaaaac tcttaaaaga
      361 caaacctcta atttatgact tagtacatga acttagagaa tttgtaactg aaagttttca
      421 agatgtaaga agggctatat ctgagttaaa acctgttgaa tatgaaaatt accaaagttt
      481 atttaaaatc aaagaattag ttaaaagctt tattaagctt acaaatatta atgttaaatt
      541 aactatttca aaaaatactt ggaacttatc cagaaatcaa tctatagctt tatatagact
      601 catacaggaa tccctttcca actcatctag gcatggaaag gcaactgaaa tcagaatttt
      661 tattacattc aatacttcaa atcttataat tactattagt gataatggta taggatgtgg
      721 caatataaaa aaaggaaatg gacttaatag tatcaatgag agaatatatg aattaaatgg
      781 taaagttgaa tttgataatt ctaataatgg tttcacaatt agagcttctt ttcctaaatt
      841 ttcagggggt gatttttttg aataaaatta aagttcttat agtagatgac gaaaaattaa
      901 ttagaaaagg tcttaaaatt atattatcat cttataatga tttagaaatt gtaggagatg
      961 cttctaatgg atatgaagct ttagaatttt gtaaaatgaa tgatgtagac atagttctta
     1021 tggatatacg aatgaaagta tgtgatgggg ttctaggtac aaggcttata aaggaatata
     1081 ataattctat aacattatta attttaacaa cttttaatga tgatgaatat ataaaagatg
     1141 ctatgaagtt tggtgcttca ggataccttt taaaagatag ctctgatgaa gtattgcatg
     1201 aagggattcg ttcttcattt tttggcaata ttgtgcttga taaaagtgta gctgaaaaaa
     1261 tcatgacctc tgaaaaaacg gttaaacaag agcatcttta tgacatgtac aatttaacag
     1321 aaaaagaaat atctataatt agactaattg caaatggact taataataaa gaaataagtc
     1381 aagaactatt cttatctgaa ggtacaataa agaataacat aactaatatt ttagctaaac
     1441 tagaacttcg tgacagaact caactagcaa tctttgcttt caaaaataaa attgtaatag
     1501 aataaatcta aaaatataaa gtgacttaag tcactttttt tagtgactta tagtaataga
     1561 attataattc acaattcttt attataatat tatagctttt taacttatac aaaattattt
     1621 tggagggatt actatggata tagtcagagt aaataatata acaaaaagat ttaatgataa
     1681 attagtactt gataacatca gcttttctgt taaaaaaggt gagatatttg gacttattgg
     1741 cccaaatggt gcaggtaaat ctacacttat aaatataatt acaaatctta tgttaccaaa
     1801 tagtggtagt attcaaatca atggtttaga tttatcaaaa gattatataa aagcaaagtc
     1861 tataataggg ttagttccac aagaattagc aattatagaa actcttactc cttttgataa
     1921 cttagagtac tttggagctt tctacggttt aaagggaaaa ctacttaaag aaagaatcat
     1981 tgaagcatta gaagttacag gtcttactga agtcaaaaag aaaaaggtca aaaaattatc
     2041 tggtggtatg caaaggagat taaatattgg aatagcactc ttaaatcatc ctaaaatatt
     2101 aatattggat gaaccaactg taggtgtaga cccccaatca agaaatcata ttttcaattt
     2161 tataaaagat atatctaaaa aacatgaaac tactgtaata tatacttccc attatatgga
     2221 agaagttgaa cacctttgtt ctaaaatttt tattatggat gaaggtaaag aaattgcttt
     2281 tggagataat gattatttaa aatcactcgt atctacaaat acaaaattga ttatggaaat
     2341 aaaaaatata aatgcccaac ttatctttga tttaaaaaat actaaaggtg ttatatctgt
     2401 tttagaaaat aattcattat tagaattgga tatagataaa aaattacaac ttacagatat
     2461 actctctgtt attgacaaaa acaattcaaa aataatgaaa atttcttatg aagaacctag
     2521 cctagaagac gtatttttaa atctaacagg aaaaaattta agggactaat ggaggtgatt
     2581 ttatgaaatt atacatgagt gtaaaaacta tgctaaagag tttaaaaagc tcctttatac
     2641 ttaatttaat atatttttta gctttgcctc tgattttatc ttggtttctt gggatggtta
     2701 cagaaagcat gtttcaaaac cctataaaaa cagaatctac ccccattgtt atatatgaca
     2761 aagataacac aagattatca aatgatttga caaagtattt gaaaaatgat ttatcttata
     2821 tattaacagt taaaaaagat gattctaagg cagaattaaa gcttactata cctaaaggct
     2881 atgagagtag tttattaaat gaaaaatcaa atactctaaa catagaaaaa cttggaactc
     2941 gtgatgatat tgcaatattg cttcaagata tattagatac ttaccatgaa aaattttatt
     3001 taaataattc tcagaaaata agttcagaag atttttctaa attatttaat aaaaattcta
     3061 ttgatacttc tataataaat aacaatgtta aacagaactc ttatgaatac tttgctttag
     3121 tttcactagg atttcttgta ataattttta taatgaacaa tattttgtca aattatataa
     3181 gtgaatcaaa gggactttca aaaagacttt attctatgcc tataactaga gtacaattct
     3241 taatttatga ctttgtaggt ttatggattt attcatttat attcttatta ttatatgttt
     3301 tattctttag aatacttgga ataacattta aaggtaattt tgccatattg ctcctacttt
     3361 gtgctttatc atcttatttt atgacttcaa tatctacctt tgtaaccagc ttttttagta
     3421 aaaaatacgg tacagcaata gtttatgcat tactcttttt acagacaatt tttggaggta
     3481 tcttcagtat gataagtgat acatttacta agcttacaag tttatctcct acctatttaa
     3541 taggagaatt gtttagtaat tatgaaacat ttaaaactat agattcaata ggagatttaa
     3601 ttttcacatg tttaattatc tctacaatat taattgcact tgctattgta aaggaaaaat
     3661 ataaatggag ggaggtttaa taatgattag attaactatt gcaaatataa aaagatatct
     3721 aaaaaatcat actcttttaa ttaatatggt tatgcttcct attatactaa ttttttcact
     3781 taattttttt atcaacaact caggaaatca atcaatgtac ttttctccag tagcaattgt
     3841 atttgactct tcaggaaagt atgaacataa gttaataaat agttctaaat taaaagaaaa
     3901 ttcatttaga ttaaatgaac aagataaggc tatgaatcta ttaaaaaata ataaggtttc
     3961 tgctgtattc gttttagata aaaatttttc aaatagtata gataaattac aacgacctgt
     4021 agttaaatgc tttaaaattg aaaatggtgg tggtagtctt tgggcagaat cacaaataga
     4081 atcttttatt actaaatcgt taaaattgaa agtggataaa aatatagatg ataaattgac
     4141 aaagacaaat ataattgata acaaaccaga taagaataaa ggttcatttt tagtagtctt
     4201 cttgatatgc tattttatgt atattaatgc ggcacatctt gcttctgatt tatttacatt
     4261 aaaaaaatca aatgtgctaa aaagacttat ctctacagat aataaagata taaaaataat
     4321 tttcagtata tttttaggac tattttttat tcaatcaatt gtatacactt ttgctctatt
     4381 atgtttttca attatagaag actttaattt gagtctgaat gtattgatga ttgtactagc
     4441 aaatagtttt gtttccactg gatttgtatt ttgggtagct agagtattta aaaatgaaag
     4501 ttctataagt cttatatcta cattttattc tttaatagat ctagctatat ctatctctag
     4561 tctaatacca tcaatggata aattatcctt tatgactaat ctttcaaagt tcacaccatt
     4621 ttattggact atagatgcta ttaaaaacaa tggtagtatt ttaataaata taatagctct
     4681 aatattaatt ggaattatat ttgtaactgc aggaagtctt aaactaagag attttgctaa
     4741 aaactaataa ttattaaata taattagtat ctatttttat ataattgtaa aagaatattt
     4801 caacatatta aattatttta tcttatattt ttctctttag tttcacacat caccaagtct
     4861 aaactttata ttatatatta atatttttga cttgtcaagt tatttatgtt ataatattaa
     4921 tcattagata attaaagcat tgtacctaag ttgcaatatc taaaattcaa tttggaggta
     4981 taactagcat atgtataaaa ttggtgaaag tgtaatgtat ccaaaagagg gagcctgctc
     5041 tgtaaatgat attgtaacaa agaaaataaa tcatgagatg caaaaatact atgaattaag
     5101 tgtcattttt aatagtaacc ttaaaatttc gattccagtt ctaaatgcag atagaattgg
     5161 catacgtcca gttatggatg gaaatgatgt tgataatttt atccaatcta ttaataaaac
     5221 agatggtgta tggatttttg atagaaaaga aagacttaaa ctgtaccaag ataaatttca
     5281 ttcaggagat gtttttgaaa ttgtaaaatt aatcaaaatg cttatgatac aagacagttc
     5341 aaaacaattg tgtagcacag ataaggagtt tttaaataaa gctcaaaaat ttgctttatc
     5401 agaattagct gctgctcaat gtaaatccta tacaatggta ttagaggaaa tgaaaaaaca
     5461 tatattaaat tcaaagaata caaattaaac cagtttttta aactggtttt attttttata
     5521 ctaatcatcc atgctacttt agcttatttt aaactgccta atctaatatt aataaagtta
     5581 tctcaattag atagatgtat tttattcaaa taaaaaaaac taagagtaaa tcatattact
     5641 gatttactct tagctaataa aatttaatct tcttcaagtt cttttttctc ttgtaataat
     5701 acacttaatt catttgcttc taaaacttgt gcctctgcaa tatcaagtaa ctcttgttgc
     5761 cattccacac atttatcttt gtaatcaaaa ggtagcgttc tgtcaactat gtacttatca
     5821 atacagtcct ccgcactttt aattatttct tttctttcat caataaattt ttctaattct
     5881 actaaggctt cttcataagc ttttctactc atataaattc cccctttgta caattattct
     5941 acattaaaat gatttttcct cttttttgta taaaatttta tttgtatata acttttccat
     6001 caatatcttt tatagtttga catccagtta atatcataga actacttagc tctgattgaa
     6061 gtttatttac ataagtttct acaccatcag ttgcaccacc aaaagaagca gtcacaaaag
     6121 gtcttcctat taatactgca tctgcaccta aacctatcat ttttagtacg tctaaaccag
     6181 ttctcactcc accatctgca agtattgtaa ctttaccttt aacagcatca gcaatttttg
     6241 gtaaaacttc acatgctcct ggtgtacagt caagcactct tcctccatga tttgatacaa
     6301 ctattgcatc cacacctgct tctactgcca ttaaagcatc ttcaacagtc attatacctt
     6361 tcaatataaa tggtagttta gtagacttta ctaactcctt tatttgttta acatttttag
     6421 gaagtactgg ttttccatgt aatgataaag ttactagacc acaggcatct atatcaactc
     6481 caactgcaaa tgcaccagct tcttcagcta atcttatctt ttttataata ttttcattat
     6541 cccaaggttt aataaataca attccagctc cattatactt tttgactaca tctaaatttt
     6601 ccattaaaaa agcatctact gccgtatcac caaccattgc ataaatacca ctattagcac
     6661 atcctgcaac tactggttct atgtaatctc tttcattaat ttttccaccc atatttaaag
     6721 tagttcctgt tactggagct gcaaatatag gtgcactcat tttctttcca aacatctcta
     6781 tagatgtatc aggatttgat acatcatgta ttactctcat atttactttt actttttcta
     6841 aactcttgct attttctata aaagaagaac cacttccttt acctcccatg ccaggcactt
     6901 cacctgcaca agcaacccca ttacaaacct tacaaacttt acaacttcca tttaaatttt
     6961 ctctagcact ttttaataat tcattatatt ccatctttaa tcccccatta actatagtaa
     7021 tttatattat atacattata tcatattata tttacagtat atttttcaaa aaactttttc
     7081 tgtggatagg agttatgcca tatttttcaa tagcttcata gtgttcttta gttccatatc
     7141 ctttgtgaga tttaaaccca tattctgggt atactctatc atattggtac attatactat
     7201 ccctagtaac tttagcaagt atacttgctg ctgctattga tatagatttt gaatctcctt
     7261 tcacaattgg attttgagat atgtcaatac ctggtattgt tgctgcatca actaataaat
     7321 aatctggtgc tttttttaaa caatttatag cctttttcat tgccatatat gtagcattta
     7381 atatattaaa ttcatctatt tcttcattgt ttactatacc tattccataa tctagtgctt
     7441 cttcttttat tacttcaaaa agttcatctc tttttgcctc actcagtttc ttagagtcat
     7501 ttacaccttc tattttggta tcttttttaa atactacaac agaagctaca acaggtccag
     7561 caagtggccc tcttcccgcc tcatcaattc ctccaatata taaataacct ttatcatagc
     7621 cttcgttctc aaatatattt atagtttcca atctttcttc ttcttttcta atattatcca
     7681 gcttttttgc cagttttata gctagacctt gtacagattt tctttcatca actcttaaca
     7741 gctctatata ttccatatat ttttctactt ctaaagtctc tattatttct ttaatctccc
     7801 tcacactttt atcctgcata ccattccctc atctttcgtt tttaaaaatt taatttttaa
     7861 atatattaaa attttttaat tagagtttaa ttgcatccac tatagccaca ttttaaacat
     7921 atactacatc cacctgtatg tgcaattcct tcacttccac actcagggca cacaaatttt
     7981 tcatcttctt caaaattttt atttattatt tctttggctt tttcatcttt attatctttt
     8041 tcttttttat tatttttata atattctttt ttactatttt cttttttatc ttctttatta
     8101 ttttcttcct ttttttcatt tattaaaatt ccactccttt tacatccatc tctaaatatt
     8161 gttactcctt taagaccagc ctcccatgcc ttaatatata tattgaaaac atcttcaact
     8221 gtagtttcaa aaggtatatt tattgtagat gatatagaag catcaatata cttttgccat
     8281 actttttgca tgtttattct ttcttcatat cctatattca tagcagtaac aaaaaaatct
     8341 ggtaaacatt cttcatctgt aatacctttc ttgtccatat actctttaac aattggagta
     8401 tatactttat aatacacatc ctcatcatgc aagctttcag tttttcttat atatgaaaga
     8461 ttaaatatag gttctattcc accactgata cctatcattg tagatatgga acctgtagga
     8521 ggtatggtta aaagttgtga atttctaagc ccatactttt taaccatttc cttagtgtct
     8581 tcatttagat tttctataaa aaacttagat ttagaaatat tttcatattt atactcacta
     8641 aatgttccat attctttagc aattaatgct gattgcttca cagcttcatt taacatagtc
     8701 ttaccaatta aatcacatag ttctaaagat tcatcagaac catatcttat tcccattttt
     8761 ataagcatat cagctatacc cattacacct aaacctatct gtctatactt atgtacacta
     8821 tctctttgtt cttgtaatgg atgcaagttc aatccctcat ctaagacatc atttaaagct
     8881 actacagctt ctctaacgca agattttaat ttattaatat caaaagtagc atgttcacca
     8941 aatggttgta ttataaactc tgacaagttt attgagccaa gtagacaact tcctccactt
     9001 ggaagtggtt cttctgcaca aggatttact cctgaaaagc aaaagttctt atcttcactc
     9061 ataaaattat atgtttttat tttatcccaa aacaatatac ctggttctgc ataatcccag
     9121 ttatttttca caaatttatt aaataaatca taagcattta ctatcttaac tattttttct
     9181 ccagtagttt caacttcaaa agtacaatta tataactttt tttctcttac agcttgcata
     9241 aattcatctg ttactcttac agaaatattt gctttagtaa ccttctccaa atctgttttt
     9301 atatctataa agtcttctat ctctggatga tttacatcca tagaaatcat aagagctcct
     9361 cttctccctc tttgaccaat taagccagtt gtcatactgt aaagttccat aaaagagact
     9421 gctccagtcg tatgttttgc agcattgtta actttcgctc cttttggtct tagctttgaa
     9481 atatcaattc caactcctcc accataacta aaagttctag caagtttttt tgcagtatca
     9541 aatatatctt ctatgctatc ttttggtggt tctattacat aacaattaga atatgttact
     9601 ttttttccct ctttctgtaa tcctctattt gctaaaattc ttccagcaaa tagaaatctt
     9661 ttttgtctta ttagttttga tattcttgaa ctattatttg aaattctctt tatccattca
     9721 tcaaaactct caccattatg tctatacttg ttcttccata ttgatttttg taattcatca
     9781 aactcccaag ctttagtcct tatttcagag cgtctttctc tatataatat gtagtgtttt
     9841 gctatatctt ttctatttga ttccataagt agtacttcaa ctatatcttg tatttcttct
     9901 acggataagc atttttccat agaattatag tcattagcta tttttcttga tatatcatat
     9961 gccaactctc tatctacgcc accttcacta tttatactag ctttatagac agcatcttca
    10021 atcttacttt tactaaaaag ttcttttgta ccatctcttt ttattatgta cttcattttt
    10081 aaacctccaa taaattagta ttaaattaat acacacatta actataactc taaacaaact
    10141 ttttatatat ttgtatattt tcat
//
LOCUS       DAENCV010000137        10153 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAENCV010000137 DAENCV010000000
VERSION     DAENCV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.139"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            17..868
                     /locus_tag="KQD82_003210"
     CDS             17..868
                     /locus_tag="KQD82_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG4381664.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            1480..2541
                     /locus_tag="KQD82_003211"
     CDS             1480..2541
                     /locus_tag="KQD82_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG4381665.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKAPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2657..3631)
                     /locus_tag="KQD82_003212"
     CDS             complement(2657..3631)
                     /locus_tag="KQD82_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381666.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            4087..4968
                     /locus_tag="KQD82_003213"
     CDS             4087..4968
                     /locus_tag="KQD82_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG4381667.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            5350..6153
                     /locus_tag="KQD82_003214"
     CDS             5350..6153
                     /locus_tag="KQD82_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381668.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            6165..7295
                     /locus_tag="KQD82_003215"
     CDS             6165..7295
                     /locus_tag="KQD82_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG4381669.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            7299..8708
                     /locus_tag="KQD82_003216"
     CDS             7299..8708
                     /locus_tag="KQD82_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381670.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            8919..9428
                     /locus_tag="KQD82_003217"
     CDS             8919..9428
                     /locus_tag="KQD82_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG4381671.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         4072 a         1057 c         1718 g         3306 t
ORIGIN      
        1 gtagaggtgg tagagtatga gttgcatttt aaaattaaaa cagatatatg aagatttaac
       61 ggaagtagat aaaaaaatag ctgattacat acttaataat acagaggcta taagtaagct
      121 ttctgtatca gaattggcgt caaactcaaa aacaagtaca gcaagtatag ttaggttttc
      181 tagaaaaatg ggatattcgg gttttgggga tttaaaaatt gagatagcta aagatttgat
      241 gggcaaagaa aatgaataca catatgtaga tgaaaactat gattgtaata tagatagtgt
      301 tataaacaaa attacaaaca aaaacataga gactatcaac caaacaaggt tgttgaatga
      361 aaaagatata ataaaagaag cagttgaaca gataataaag gctaaaaatg tatatatatt
      421 tggagtaggt ggttcggctt tagttgcact agatttacag atgaaacttt taaggataaa
      481 taaacaggca tttacctctt tagatagtca tacacaactt atggtatctt caaatgtaga
      541 taaagaagat atagctatag caatatctta ctctggagaa tcaaaagaag taataaagtc
      601 tatagaaaat gcaaaattaa aaggatgtaa ggtcatttgt ataactaaat atagtgacaa
      661 tcatcttagt aagattagtg atttaaagct agtagtacca aatatagaaa aaagacttag
      721 agaaggagct atttcttcaa gaatagcaat gcttacgttg atagatataa tatatatttc
      781 tataattcaa gaaaacttaa atgaggcaga acagaaacta caagaaacaa aaaggatact
      841 tgattattta aatattaata atgcttaaaa ctaggttata tatcaactaa acaaataagt
      901 taggctataa gcgtaagtca ataagaaata gataaatatc taaaatattg acttacttaa
      961 taataatata tagcattttt ataatacaaa tgagttgtct taaaattgat tttttttaat
     1021 caaattttaa gttcaactcc ttttttatat ttttatagat atatcctata ttttcccaga
     1081 aaacgtattg aatatacaaa ttataagaag tataatgttg ttgggagaga atgtatgaaa
     1141 tgtatacaaa gttgatttta actgtattat tgataattct ctttactctt accattatta
     1201 atgattgaag acaatggtat ttattatagg tcaaagattc cgaatgtatt aatcatcatc
     1261 attttctcaa actaacagca aacaccctga tttgcgagtt gtattgtata caaattatct
     1321 agaaaatatc tattagattt tccatgaaat ttaaatattt cacgaatgta tgaatggaga
     1381 aatatacatg ttgacaatcc acctttttaa atcctaaatt aattttatta atggtagaga
     1441 ataggaagta acaatgtttt ataaaggaga atattaagta tgaagaaaat taggaagtta
     1501 gctaccaagt tattgattac aacaatagtt ttaatctcag gtatgagtat gacagtttat
     1561 ggtatgacag ccaaagaggt aacagcaaaa gcaccaaaaa gctatgtcac tggaaccaat
     1621 agcgtatatg gtccaaagct gtcacaagcc caactaaact cagtagcaca agcaacagca
     1681 gattttatga acaagaaaat cacaaagaat atgaccacag atgctaagat tttagttgcc
     1741 tacaatcaca ttaaaaataa cacaacatat gttgactgga atgcagtaga gggagcaaat
     1801 actgcctata cattggttac taaaaaaggt gcatgctcag gaatggctag gagcatgaaa
     1861 gctttgtgtg atgctatggg aattgaatct tattatgtac acagcacatc aaatgaccac
     1921 cagtggaatc ttatccgttt tggtgatggg cgtttgtatc atgtagatat tgatgctaat
     1981 aaatcagcag gaaaggatat catatacaag agtcttagcc acccattacc atttgataag
     2041 acagcatatc caaaagttgg tgcaatagta cagacggtaa aaccagaaca gacacagaaa
     2101 ccagcaccac ctgggaagca cactatgtta ccacatcaag aaggggcaaa ggctgttgga
     2161 aattttggaa aaaaaggaga agatataaat gaaaaacatg taatgtatga aggtgttaga
     2221 tgtagtgtag ttaattcatc agtaaataca attacattta caggtagtga atcaaattta
     2281 gacccatcaa atcttacgat tgctgtaata ggatttatga acacaccata tgggatgttt
     2341 gcattagcac cagagggtaa acctgttgaa tatcctttta aatatgataa gccattttct
     2401 ttagatttta gtggtaaaaa tcgttctggt gcagaattag cgaagtactg taaaaaaaat
     2461 gatgtgacta ttaaaataga aatcactgat gaaaaagcta atattggact tggtcaaata
     2521 atgtatgttc gatatgaata atgatttaat catgacagta gaataagtta taaacattgc
     2581 gccctcttac taggggcgca tttaatatta tatctgatat tttgtagtca ggtcaactgc
     2641 tagatttctc ccctttttat ttataaaaat tattatctct aagcatctga gcatgtttac
     2701 gattaactaa aaactgtttt aactcagtca agcataggaa taaaatagct ctattttcaa
     2761 actctgttct gtcaacaggt agaggctggt ttttcatact aagcattagt ctctttaatt
     2821 cttcaagaag aagattgcct gtttcagctt caaattcagt ataacctatc ttatgtataa
     2881 aagcagatag aatatgagct tgtggtggtg tagaatttag ttgagataca taactataca
     2941 aattttctaa gatatcccgt tgacttttta taatatccat atgctctaag aagaatctag
     3001 tatcacttaa taaagtatta ttaatattat cgtaagtttc agaaatactg ctgtcaataa
     3061 gtgaatttaa tgtatataaa tctctactat atccattttc ttttttagga tttacaataa
     3121 tatctgcaat gtctataagt acaatactca cttcttcctg taactttttt tgaacttcat
     3181 ggattttatg tatatttgtt ggaatataca tatttattaa gacacctatt ccagttccta
     3241 ctgcaaatat tccagcttca tttaaaatcc atttgataga agtttctcct tgaaggtagt
     3301 aatgggatgc tatgactaca cacatagcaa taacatcttg aatatttaac aaaaaacaaa
     3361 gtggtataat tataaatata aatattgaaa atgaagtcaa attatatcct aaaatattaa
     3421 aacataaata tgaaaaaata gtacataaaa gaaaaccata aatctttcca atggagcctt
     3481 taagagtttc tcttttagtg tcctttactg taagtagtac tataactcca gcaaccattg
     3541 aatattctaa ttttaatagc caagcaataa atatagcaag agaacatcca agagataatt
     3601 ttattacttt aagtgaatta atattattca aatgcaaacc tcctgtaaat caatataata
     3661 aaattctaac ataaaactaa tattgcttga aaggtaagta actatactta attattttat
     3721 tgccctattt gtttagatac aatcaatata attttatttt atgaaaattt tttgtaaaat
     3781 tttaaattta gagactaaca tgagaccaca aagaggaagt ttattatagg aagaaaactt
     3841 aaaattgatt ttaagcagca aatgaaaata atcaaatatg tatatactat tattaaaaaa
     3901 taaaattata tagttaatat aattttataa ttggtttagt atagagattt ttaactttta
     3961 tgttatacta tttataaaag taggtataaa gattaaaata cataatcaaa tatgtataag
     4021 aggttttaaa tagtattgtt aatgaacttg attattgttt ataattacaa attggaggag
     4081 atagacatga aaattactga aggatatatg ccttttaaag gttttaaaac gtattaccgt
     4141 atagtaggag aaaatacgga agggaagaag ccattagtat tacttcatgg aggtccaggt
     4201 tctacacaca actattttga ggtattggat aagatagctg aaagtggtag acaagtaata
     4261 atgtatgacc aaattggttg tggaaattca tttgtagaag ggcatcctga gctatttaat
     4321 gcagatactt ggatagagga gcttattgaa ctaagaaaac atctaggact tgatgaaatt
     4381 catctgttag gtcaatcttg gggaggtatg caggctattt ggtatgctat tgaatataaa
     4441 ccaaaaggta ttaagtctta catcctttca tctactcttt cttctgcaaa actttgggag
     4501 aaagaacaaa aaagaagaat atcatatatg agtgaggttg accaaaaagc attacttgat
     4561 gcagtaaata ctggagatta ctctagcaaa gaatataatg atgcattaga aagatttatg
     4621 gaaatgtatt gtgcaggtga agtgacagaa gattctccag agtgcctgag aagacctaaa
     4681 aaatcaggtt ctgaagcata tattgtagga tggggacaaa atgaattttc tcctacagga
     4741 actctttctg gatatgaatt tacagataga ttacatgaaa taaaagagcc ttgtctagta
     4801 actagtggag caatagattt atgttcacca tatattgcaa agactatgta tgatagaata
     4861 cctaatagta agtgggaatt atttgagtat tcaagacata tgccattcgt tgaagaaaat
     4921 gagaaatata taaaagtctt aactgagtgg ttgaatgcga acgattaaaa actatataaa
     4981 tttatataaa aaaatgagtg tctcaaaatg aactttttag ttcattaggc actctttttg
     5041 catgaaatca aatatatttt cattatttga acgataaaat gaagcttata tctattttgg
     5101 gaaagtaatt ttttattact agcgttagat tctaatctaa ttgtatactt attgaaataa
     5161 tagaaatata ttggtgttta taacttaatt ttattgtttt ttagaaattt ttttatatat
     5221 tggtataatt attctgttta aatttgatta aaaagtatat taattataat aagaaacaat
     5281 tatataaata ggaatagatt attataatta atatagatat aaaaataatt atagaaggaa
     5341 aggcacatta tggaaaaatt gagagttatt gaaatgataa aaaataatcc caatattata
     5401 gctacaatag ataatccaac tgatgaaatg aaattgttag ctattaaaga aaatgggctt
     5461 gtactagaat atataaataa tccaaccaga gagatgcaag aactagcaat agataataat
     5521 attagagcaa ttaaatttat agataatcca acagaagata tgatgataaa agcagtaaat
     5581 gagggatgga gtatactgga ttatatcaaa aacccaactg ataaagttat tgaaatggct
     5641 ataaatcaag caggatgggc aattaaatat gctaaaaatc caagtgaaga attacaacta
     5701 ttagcagtaa gaaaaaatta tgattcgata aagtttataa aacaacctta tgaaagtgta
     5761 caagaggagg cagtgagaat aagttatgat gccttgagat atatagattc acctagttac
     5821 aatgcagaac ttatagctat aaaaaataat gaagcagcta ttagctttat aactgattta
     5881 gataaaaata aaatgttgga atttttaaaa gtgaatattt tagtgattaa gtacattctg
     5941 aaaaaagtat ctaaagatga attagagagt gtattaaaag aagtattatc caaagaagat
     6001 gttgaggaaa agtatattag agattttttg aattgtagtg ttatagatag aaatagcgaa
     6061 agtataccaa tagataaaat tatgtttatt tataaatatg gaagtaagac agcaaaaaaa
     6121 atagctgtag atgagaaatt aaagattatg taggagagag aaaaatgaat tttatagaca
     6181 cattaaaaag cgttgactta atattatcca ctggagaagt acctttaata gtaggtgaaa
     6241 gtggaatagg aaaaacagct ttagcaaata agcttgccaa agaaaatgat tggagtttaa
     6301 ttgttattga tggaaactta cttaaagagg gtgaaatagg tggtcttcca actatagaat
     6361 cttatgtagg agttaattct aatggatata agactgaaaa gaaaacaaca gtgtatgctg
     6421 ttcataacaa attaagagaa attgatgaag agatatctaa aagtaagaca gttcttttat
     6481 ttatagatga gataaatcgt tgtgagcata cagtacagca agaacttatg aatttaattt
     6541 taaatagaga aattaatgga tataaactgc atgatgatgt aaagatttta gcagcaatga
     6601 atccatcaag taaatatggt tcagattttg attatcaagt ggtagatatg gatgcagccc
     6661 aagaaaatag gtttgtttgg ttaaatatgg agagtgacca tacacaatgg ataaaatggg
     6721 caatagatga gggtattgaa agaaaagtta tcgagtttat ttccactttt ccagaatatc
     6781 tgcataaaat aaatgaagat gatgtaagag ccactcctag aagttatgag agagtatcta
     6841 aaatttataa agtttataaa gaaaaaaata attctattcc tagagcagta tttttaaatg
     6901 ttgtaaaggg taatgtaggt aaagttattg cagaagaatt tataagtttt attgaatcaa
     6961 actctgaacc attaatatcc tatgaagatg tttttttagg agaatctatt gatgagtcta
     7021 ttgtagaaag agtaaaaaat gagagtcata caagacttta tctatcagct atgaacattc
     7081 taaaagactt agaattaaat attaagaatg ataaatatga atcaaaccac tatattaata
     7141 gatttattga atttttaaaa atgtatcctg tagatttaat gataggtatt atgaaggata
     7201 ttagaaacag ttatattgaa gtatataaaa aagctataga aaatgaagag tttgtaaaat
     7261 catattttga atcttatagt ttaataaggg gataatgtat ggaaaattat tttgataaac
     7321 aagcaaaaga gctttataat aaagcaagtg aaattataaa tactcattct atgctaaagg
     7381 caaatcgtgc taatgagaaa tttgatatag atataccaca agattttaag tatgagtttt
     7441 ttagtcttgt agataaagtg aatttaagtc ttatggaaga agaagataat ttttatggat
     7501 attttttgtt tcagatgtca agagaaataa gatttgatat aagtagtcct acaggtgtga
     7561 attttaaggg agctaaatat gttatatatt ttaatccaat aatcttttta acgcttgaca
     7621 taaagcaaat ggagactaca attaaacatg aaattcatca tatattatct atgcatttga
     7681 tgagagcaaa ggagttaaaa ggaaagtata gtacattagc aattaatatg gcaatggata
     7741 tagtagtaaa taaatttcta aacaatttac caccatatgc aactacctta gagtgggtaa
     7801 atttaaagta ttccttaaaa cttgaaccat atgaaccttt tgaatattat gtagaaaaga
     7861 ttcagactga attagatttg atggaagtgg atgaagatgg tgaggaagat gatagtgata
     7921 aatatggaga tatagaaatt gattatagcc ccgaaagaac tcatgatata tgggatgaat
     7981 ccaatgagat agatgaaaat acccttagag aatttactga aaaatttatt aacagttctc
     8041 aaaaaggtaa agttcctgct tatttagaaa gtatgatatc atcacttaaa aatagtaagg
     8101 gtgaattgcc atggaattta tatcttaata gaataatggg aacagttgaa aataataaaa
     8161 agaagactat aacaagaaga aatagaagac aaccaaatcg tttagatttg aggggagaac
     8221 ttagaagtca taagtcagaa atagcagttg cacttgatgt aagtggaagt attagtgatg
     8281 aagagtttaa acaagctatt aaagaagttc ttagtatagt gaaaaactat aatcatgaaa
     8341 ttacaattat agaatgtgat aatgaaatta gacgtgtata taaagtgaga actgaaaaag
     8401 atataaaaga tagaattagg ataggtggag gcactagatt ttctccagtc tttgagtatg
     8461 cgaataataa aaagattaat ttgttagtct actttactga tggtaaaggt gaaaataaac
     8521 tacaagtaat acctagaggg tataaaattt tatgggttat ttctggaaga ggagataaaa
     8581 tttcattaaa agaaccttat ggagcagtta aaaaacttac taaggttaaa acaaatgatg
     8641 aaatattaga aataaatgat gtaagaaatg atgggtactc aatgaatagc caagcaccaa
     8701 ttttataggt attcacagga gataagcttt attaatttat tttgatatgc ttgttataaa
     8761 aatctaaaaa atacattaat caatggttaa tgtatttttt aattttaata atataagttt
     8821 tacttacatt taagtcttaa aatgtgttaa aatataatat atagcagata gttttggcaa
     8881 ggtggattta gttgtcaaaa ctattttttt tattaaggat ggtgatgttt ttgtttaaga
     8941 ttgatgatta tataatgtat ggtatgacag gagtctgcaa agtcatggat ataacaaatg
     9001 aacgatttac aaatggaata aaaaaagagt attatgtatt gagtcctatt tattctaata
     9061 atacagtaat aaaaattcct gtagataatg aaaaagtacc aatgagaaag ttactttcta
     9121 aaattaatgt gctttcatta ataaatgata ttcctaatat ggatacatca tggatagata
     9181 atgaaaaatt gagaagtgag caatttaaaa aaatactcag aggtggtaaa tgtgaagagt
     9241 taataaaatt agtaagaagt atagataata acagagagta tgtaaaatct attggtaaaa
     9301 aaacacatca agcagatgat aatataatga aagaagctga aaggttatta aatgaagaat
     9361 ttgcaactat tttagatatt tctcctaatg aagttagttc atatatttca agtcatatac
     9421 ctcaataaag tgttttagta tcctttattt aatctatttt aagtattatt tatgtatgct
     9481 ggttttggta aaataaaata tataattaaa ttatataaaa aataataatg attgtattga
     9541 agaattacag aatagtgcgg agagcctact cctttaggtg tgagatggat atcacttaaa
     9601 gatttaaatt gttattgtag atgtttttaa tataaaacaa gattaataag tgtattgtaa
     9661 gctttaagtg aaattgatac tataatgagt tacttagatt ttaaaatata taatattata
     9721 aaaaatcctt ttaggttttg aatgaataag atggatacta tgttgaatag aatccatctt
     9781 atttaagttt tatttatgtt gtttatttgt aagcattatt tttgtacatg aaaacacagt
     9841 gtaaaaatat gcttagaatt atgaagtttt attaaaaaga tattcgacaa aataagacta
     9901 aaaatattct taaacttata actaagataa atattgtata tttatagtgt agcctgaaaa
     9961 attgtgaatg tacgaaattt atttagtatt ttattgaaac aaagtagaag aagtactata
    10021 gtttatcttt gaatagattg ttatttttta gtgaaaaagt tattaggtta atacataaat
    10081 ataatagctt ataaatagtt taagcatgtt aaaaaagtag ttattagaga tatatagtta
    10141 cattgcttat aag
//
LOCUS       DAENCV010000138         9931 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAENCV010000138 DAENCV010000000
VERSION     DAENCV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.140"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..1033
                     /locus_tag="KQD82_003218"
     CDS             <1..1033
                     /locus_tag="KQD82_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG4381672.1"
                     /translation="SKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEYI
                     ENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALE
                     YVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRI
                     CLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQY
                     VNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDI
                     CIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEY
                     VKIAIDDCI"
     gene            1172..1624
                     /gene="tsaE"
                     /locus_tag="KQD82_003219"
     CDS             1172..1624
                     /gene="tsaE"
                     /locus_tag="KQD82_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG4381673.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1624..2340
                     /gene="tsaB"
                     /locus_tag="KQD82_003220"
     CDS             1624..2340
                     /gene="tsaB"
                     /locus_tag="KQD82_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG4381674.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVDFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2330..2806
                     /gene="rimI"
                     /locus_tag="KQD82_003221"
     CDS             2330..2806
                     /gene="rimI"
                     /locus_tag="KQD82_003221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG4381675.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2812..3828
                     /gene="tsaD"
                     /locus_tag="KQD82_003222"
     CDS             2812..3828
                     /gene="tsaD"
                     /locus_tag="KQD82_003222"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG4381676.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4125..6833
                     /gene="hpdB"
                     /locus_tag="KQD82_003223"
     CDS             4125..6833
                     /gene="hpdB"
                     /locus_tag="KQD82_003223"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG4381677.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEIKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6837..7094
                     /gene="hpdC"
                     /locus_tag="KQD82_003224"
     CDS             6837..7094
                     /gene="hpdC"
                     /locus_tag="KQD82_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG4381678.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7087..8037
                     /gene="hpdA"
                     /locus_tag="KQD82_003225"
     CDS             7087..8037
                     /gene="hpdA"
                     /locus_tag="KQD82_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG4381679.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNVHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8352..9314
                     /locus_tag="KQD82_003226"
     CDS             8352..9314
                     /locus_tag="KQD82_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4381680.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9413..>9931)
                     /locus_tag="KQD82_003227"
     CDS             complement(9413..>9931)
                     /locus_tag="KQD82_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4381681.1"
                     /translation="MISVIVPTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSE
                     EYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIV
                     GISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVP
                     FLFTKEYKNNLE"
BASE COUNT         3881 a         1038 c         1857 g         3155 t
ORIGIN      
        1 atctaaaaaa ataaaaaaat tatttttaga tgataaaggg tattatttta atagtaattg
       61 gaaagatatt catattggta tttatgatac aaaattagga gattctttaa agtatatcac
      121 taatgaatat attgaaaata tggaaagggt aaaatacgat tatcgtgaat taaaaaatat
      181 agaaaatcaa actgatggta tgtgtatttt agcaataagg cagaattaca aggcattaga
      241 gtatgtaaaa aatcaaaccg aagaattatg tttagaagca ataaagtata attataaagc
      301 acttgaatat gtaaaagaac aaacagaata cttgtgtctt gaagctataa aaaaagattg
      361 taatgcactt aagtatgtac gaaataaaac agaagggctt attataaaag caataagtca
      421 ttcttccaat atagatgttg taagtatact aaaggcttta gagactcaaa caagaaggat
      481 atgtttagaa gcaattaaaa aagatggcag gtgtcttgca tacgttagag aacagagtga
      541 agaattatgt atagaggcca ttaagcaaaa ttataaagca ttaaaatatg taaaaaatca
      601 aacggaaaaa atgtgtatag aatccgtaag acaaaatggt atggcattac aatatgtaaa
      661 taaacaaact gataaaatat gtatagaagc tgtcaaacaa gatggaagaa gtatacaatt
      721 tgtcaacaat aagacagaag aaatatgtat aaatgctata agatatttaa ataaaaaata
      781 taatattaaa gatgtactaa gttacataga taagtataca gaagatatat gtatagagat
      841 tgtaagacaa aatggaaaaa tgcttatgta tataaaaaat caaacagaaa aaatgtgtat
      901 tgaagcagta aaagagaact ataagtctct taagtatgta aaagagcaat ctgagagaat
      961 atgtaaagaa gcattgaagc aaaaccataa agcaaaagag tatgtaaaaa ttgctattga
     1021 tgattgtata taaggtgtag atacagccat caatagtgaa agataaaaaa agataaagac
     1081 aagtacttta taataatcta ggcttatact ataattaatt aagcagtgat ataaattcac
     1141 atgacgatta tatcaaaatg gagatgaatt gatggcaaaa atatatttag aaaatgaaaa
     1201 taaaactaga gaaataggat ataaattagg taaactttta aaagaaggca gtgttatctg
     1261 tttagtagga gatttaggag ctggtaaaac tacaatgaca caaagtttgg cagattcatt
     1321 agggatagaa gattatataa caagtcctac ttttactata ataaatgaat atgaaggaaa
     1381 aatacctttg tatcattttg atgtttatag aataggtagt agtgacgaaa tgtatgatat
     1441 tggttatgat gaatatgtaa attcaaatgg aatctgtata atagaatggg ctaatctaat
     1501 agaagatata cttccaaaag aatatttgaa tatagaattg agatataaag atgaaggaag
     1561 agaaatgata ttaactccaa aaggagagtt ttataaagag atagttgagg agttgattaa
     1621 ataatgaaaa tactaggtat ggatacgtct tctatggcag caagtgtagc agttgttgaa
     1681 gatgataact taatatgtga gtttacagta aacaacaaaa aaactcattc acaaaagctt
     1741 atgccaatga tagaaaatat gttgagtatg agtgatttaa gtataaaaga tatggattta
     1801 ttagctgtat gtataggtcc aggttcgttt acaggactta gaataggtat ggctacagta
     1861 aaagctatgg cccatgtaaa taatatacca ataatagcag taaattcttt ggaatctcta
     1921 gcaaataaca taaatttctg tgatagaaag atatgttgta tattagatgc gcagaaaaat
     1981 caagtttatt cagctaaata caagtttgaa aatggagaga tgatagaatt agatggtgtt
     2041 gatgttgtag attttgatgc tttagtagat gaaattgtat caactaatga agaatttata
     2101 atagttggag aagctgtata caaatataaa gacaagttag agaatataaa aaatataaaa
     2161 ataccttcac cagctaataa tgtatctaac gcaggaagct tatgctcttt agcattaaat
     2221 aaatataata aaaatataga tgtacatact tgttatacga taaatcctat gtatatcaga
     2281 aaatctcaag ctgaagtaca atatgatgaa aaaatgaaga ggctaaataa tggaagataa
     2341 tacaaaacta atagtagatg atataaaaat agaagaaatg actacagaag atatagatga
     2401 agtttttgaa gtagaaaaaa attgttttga agattattgg tcaaaagagt cttttagaaa
     2461 agagttgtct aatgaagtag caaggtatat agtagctaag ttaaatggaa aaattgtcgg
     2521 atatgtggga atttggctta tacttgatga agggcatata aataatgtgg ctgttcatag
     2581 tgactataga gggaaaaaaa taggggacaa gttgataaaa ggcatagttg atttatgtaa
     2641 agataataat attgcatcta tgacattaga agttagagca tcaaataaaa tagcacaaaa
     2701 tttatataga aaatatggat ttaaaatggg tggaataaga aaagaatact acagtgataa
     2761 taaagaagat gcaattataa tgtggaacca attaaaagag gtgtagagtt tatgagtgat
     2821 ataataacat tagctataga gagtagttgt gacgaaacag ctgcatcagt tttaaaaaat
     2881 ggtagagaaa tactttcaaa cataatctct actcaaatcg agacacataa aaaatttggt
     2941 ggtgtagttc cagaagtggc ctcaagaaaa catgtggaaa atattgatat agttgttcaa
     3001 gaagctttag acaaagcaaa tataggtttt aatgatatag accatatcgc agttacatat
     3061 ggacctgggc tagtaggtgc tttattggtt ggtctatctt atgctaaagc acttgcatat
     3121 actttgaata ttccacttgt aggagtaaat catatagaag ggcatctaag tgcaaactat
     3181 atagagcata aggatttaaa acctcctttt ataacattaa tagtatctgg aggtcatact
     3241 cacttggttg aagtaaaaga ttatggcaag tatgaaattt taggaaaaac aagagatgat
     3301 gcatctggag aagcatttga taagatttca agagcgatga atttaggcta tccaggaggt
     3361 cctattattg ataatctggc caagaatgga aataaacatg ctatagaatt tccaagagcc
     3421 tatttagaag aagatagcta tgattttagt tttagtggat taaaatcttc tgtattaaat
     3481 tatctaaatg gaaaaagaat gaaaaatgaa gaaatagtag ttgaagatgt agcagcatct
     3541 ttccaagagg ctgtagtaga ggttttatct actaaagcac taaaagcagt aaaagacaaa
     3601 ggatacaata tcataactct ttctggtggg gttgcttcta attcggggct tagagctaaa
     3661 ataacagagc ttgctaaaga caatggtatt actgttaagt atcctcctct aattttatgc
     3721 acagataatg cagctatgat agggtgtgca ggatattata attttataaa tgggaaaacg
     3781 catgatatga gtttaaatgc agttccaaat ttaaagataa atcagtagag ttagatgaat
     3841 ttgtaaatat aaaagtattg tttcaaaaaa gtaagtagaa gtgaaattaa atataataaa
     3901 aataaatata gtatatatta aaacttgaag taaaactatt tataatcttt atattccaat
     3961 gaattgcaac aaattttagg atatacagat tgtctttggc ttagatttgc ttcaagttta
     4021 tttttttcta tagaaagatt attttaaaaa gtgggtaata tacccttttt tttcattctt
     4081 aaaaagtttt atactaatta tagaaagatt aaggagggga agaaatgagt caaagtaaag
     4141 aagacaaaat aagaagtatt ttggaagcaa aaaatattaa atcaaatttt caaaataaag
     4201 aaaatttaag tgaatttaat gaaaaaaaag caagtaaaag agcagaagac ttattagatg
     4261 tatattataa tacattatct actgctgata tggaatttcc atattggtat aatagagaat
     4321 atagaaaatc agatggtgat ataccagtag ttagaagagc taaagctctt aaagctgctt
     4381 tttcacatat gacaccaaat ataatacctg gtgagaaaat agtaatgcaa aagacacgcc
     4441 attatagagg ttcttttcca atgccatggg taagtgaaag tttctttgtt gctcaaggag
     4501 aacaaatgag agaagaggca aagaaattag ccagtaacac agctgatgaa cttacaaaat
     4561 ttgggtctgg tggaggaaat gttactgaaa gttttggaaa tgtcgtttca atagctggta
     4621 aattcggaat gagaaaagaa gaagtacctg tacttgtaaa aatggcaaag gaatgggttg
     4681 gaaagtctgt tgaagattta ggttttcact atgaaaaaat gatgccagat tatgatttaa
     4741 aagaaaacct aatgagtaca cttatatgca tgtttgattc ggggtataca cttcctcaag
     4801 gtagagaagt tataaattat ttttatcctt taaattatgg attagatgga attatagaaa
     4861 tggcaaaaga atgtaaaaag gcagttgcag gaaatgcatc tggagatggg cttattggta
     4921 tggaccgtct atatttttat gaggctgtta ttcaagttat tgaaggatta caaacttgga
     4981 ttttaaatta tgcaaaacat gcaaaatatt tagaaagtat agagacagat ttagaagcta
     5041 aaaaagaata ctcagattta gtggagatat tagaacatat tgcacataaa caacctcgta
     5101 catttaggga agctcttcag ttgacttata caattcatat agcatcagta aatgaggatg
     5161 ctatatctgg tatgtctata ggacgttttg gtcaaatttt atatccttgg tatgaacaag
     5221 atatagaaaa aggactaatt acaaaagaag aggttatcga attattagag ctttatagaa
     5281 taaaaatcac ttgtattgac tgttttgctt cagcgggggt aaatggagga gtactatcag
     5341 gtaatacatt taatacttta tctataggtg gattaaaaga agatggttct acaggagcta
     5401 atgaactaga agagttatta ttagaagcaa gtatgagatg tagaactcct caacctagtt
     5461 taactatgct atatgatgaa aagcttccag aagatttctt aatgaaagct gctgaatgta
     5521 caaagcttgg ttctggttat ccagcatggg taaacaactc aaatggaaca acatttatga
     5581 tgaaacaatt tgctgatgaa ggtatgactg ttgaagaagc aagagcattt gcattaggtg
     5641 gatgtttgga aacgtctcca gggtgttgga aacagttaac tttaaacgga aaaacgtact
     5701 ctattgcagg tggagcaggt caatctgctg gctctggagt tcactttata gctaatccaa
     5761 aaattttaga acttgttctt atgaatggta aagactatcg tatgaatata caagtatttg
     5821 agcctcataa taagccatta gatacttatg aagaagtcat agaagtattt aaagattatt
     5881 ataaacaagc aataaatgtt ttagaaagag caaataacat tgagttagat atatggagaa
     5941 aatttgatac ttctataata aattcactac taaagccaga ctgtttagac aaaggtcaac
     6001 atataggaaa tatgggatat cgttataatg caacattaaa cgtagaaact tgtggtactg
     6061 ttactatggt caattcattt gcagcgctta agaaacttgt atatgatgac aaagcattta
     6121 caatagagga aattaaggat gccatattaa ataattttgg atttaaagat gcattagaag
     6181 taggaaatta ttctatggca gaccaagtaa aagtagataa gactggtaaa tatgatgcta
     6241 tttataaggc ctgtcttgat gcacctaaat atggaaataa tgatttgtat gcagataata
     6301 ttcttaaaaa ctatgaggta tggttatcaa aagtatgtga agaagcacaa tctttatatg
     6361 ctaagaagat gtacccttgc caaatatctg tttctacaca tgggccacag ggagctgcta
     6421 ccttggctac tccagatggt agactaagtg gaacaacata ttcagatggt tcagtctctg
     6481 catatgcagg aacagataaa aatggagtat atgcattatt tgaatctgca actatttggg
     6541 accaagcagt agttcaaaat tctcagatga atttaaaact tcatccaact actataaaag
     6601 gacaacaagg aactaaaaaa ttattagatt taacaagaag ttatttaaga aaaggtgggt
     6661 tccatattca atataatgtt gtagattcag aaaccttaaa agatgcacaa aaaaatcctg
     6721 acaattatcg tcaattaatg gtacgtgttg ctgggtttac tcaatattgg tgtgaattag
     6781 gtaaaccaat acaagatgaa gtaattgcta gaacagagta tgaaggggtg taaaatatga
     6841 gaaagcatag tgattgtatg aatttttgtg cagtagatgc aaccaaagga atttgtagat
     6901 tatcaaaaca aatgattaat ttagatgatg cagcatgtcc agagataaaa gtaatgccaa
     6961 aatgtaaaaa ttgtaaaaat tttgttgaag ctaatgatga aggtataggc aaatgtgttg
     7021 gcctagagaa agaagattgg gtatattcaa cattgaatgc aattacttgt gaaggacatg
     7081 tgtttaatga gtagtcaaaa gcaattagaa ggcatgattt tcgatgtaca aagcttttca
     7141 gttcatgatg gtccaggttg tagaacaact gtatttttaa atggatgtcc actgagttgt
     7201 aaatggtgtg caaatccaga aagttggact gtaagaccac atatgatgtt tagtgaatta
     7261 tcttgtcaat atgaaaatgg atgtactgta tgtcatggta agtgtaaaaa tggtgcattg
     7321 agctttaatc ttgataataa gccagttata gattggaaca tctgtaagga ctgtgaaagt
     7381 tttgaatgcg tcaactcatg ttattataat gcgtttaaat tatgtgcaaa accttacact
     7441 gtagatgaac ttgtgcaggt aattaaacgt gattctaaca attggagaag taatggagga
     7501 gtaacattta gtggaggtga gccactactt cagcatgaat ttttacatga agtattatta
     7561 aagtgtcatg aggtaaatgt acacactgcc attgaaacga gtgcatgtgt atcaaatgaa
     7621 gtttttaata aaatatttaa cgatattgat tttgcattta ttgatataaa gcatatggat
     7681 agagaaaaac acaaagaaca aacaggtgtg tataatgatt taatattaga aaatatatca
     7741 aaccttgcaa attctgattg gaatggaaga ttagtactta gagtaccagt tatctctggg
     7801 tttaatgata gtgatgaaaa tataagtgat attatatctt ttatgcacaa aaataatttg
     7861 gtagaaatta atctattacc atttcacaga ttaggagaat caaaatggac tcaattaggc
     7921 aaggaatatg agtattctga taagggtgat gttgatgaag gtcatcttga agaattacaa
     7981 gatatatttt tggataatgg tatagcttgt tatgttggtc atgagacagc tttctaaaat
     8041 taaatacaag ttttaattaa aaaaggatgt tacactaaaa gctagagtta catccttttt
     8101 tatgtggttt ttatgtagaa ataattaagt aaagttttat atgttttata tattgtttat
     8161 attgctttat gtccataaat attgctttaa atatacttta tatatttttt atagtttaaa
     8221 ttacagttag agaatatatt acgtttactt tattgaagca gaagttttgt ggtaaaatac
     8281 tactatgaaa aaatttaata gaatatattt tagaaacaac taaaaataat agttaggagg
     8341 aagacatcta tatgaacaaa tcatggagca acttgattat taagaataaa gacagaaaag
     8401 tacgttcagg gtggattatt atagctgtta tggctacctt ttacatactt cagtattatt
     8461 taagcatgat acttgtggaa ataataagaa aaatcctggt tgctacaggt gacattaatt
     8521 taaaaacaaa ctatttttca gaatatgcta attggttaaa tgatgttggg ttacagattg
     8581 tatttcaaat tctaacagat tcacttatga ttattattcc tataattgta tggagattta
     8641 ttatgaaaaa tcctattagt gaaatgggat tacattctta tagaattaag aaaaaagaag
     8701 gatatgtagg aatgttatta ggaatattta attgtacagt aatctttatt ataacaataa
     8761 caattggtgg tggtcatgtt acttcatggg taccaaaaat ttcaccttta acagtttctt
     8821 ggatactggt ctttatttta gttgcttttg cagaagaaat tttaaatcgt ggttttttta
     8881 tggcagtttt acgaagatgt aaaaatattt attttataat gattgttcca tctgttattt
     8941 ttgggttgat tcatatatgg aatcctgatg ttactttttt gtctgtaatt aatattataa
     9001 ttattgggat tcttttttca tatatgttta taaagtcgtc gaatatatgg atgtgtatag
     9061 gttatcattt tacatggaat gtatttcagg ggattatata tggtatgcct gtaagtggac
     9121 ttcaggttcc agggattata actacacaaa tcactcatgg taatctatta aatggaggga
     9181 tgtttggtat tgaaggtgga attttgacta cttttgttaa tctacttagc tttatatttg
     9241 tatggtatta ctatagagat tctaagtatg attttgttag tgataaagta aatgtgacca
     9301 atatcaagaa ttagttgatt ataataaatt ttaataatat cttctaagct taaagtattc
     9361 tgtaggattg ttggaaattt gcaaaaatat aaaaataact tggatattct atttactcca
     9421 agttattttt atattctttt gtaaatagaa atggaacata aaaaattaaa acaaccatag
     9481 atactattag tatatcatgg taagtgatag ttgattcttt agaatttgtg ataagaaaag
     9541 cactattatt taaaacatga attatagcac aaaaccagat attcttagtt tttatataaa
     9601 gatatcctaa aaatattgaa attccaacta tatgtattga acgtaatatt attcctaata
     9661 ttggcgcttc aggtgtatat aatgtaaata ttagtggtaa gtgccacaag ctccaacaca
     9721 tacctaatat tataactcct aactttttac caaatttatc aaaaaaaata ttctgtaaaa
     9781 aacctctcca ggcatattct tcactaaaaa aaatataaat agtagttaat gatgttattg
     9841 gagtaaatat aaattttatg ataccactat aattaggttg agccccttgt agtattagta
     9901 taaatgatgt tggaacaatt acagaaatca t
//
LOCUS       DAENCV010000139         9825 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAENCV010000139 DAENCV010000000
VERSION     DAENCV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.141"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            337..1266
                     /gene="glsA"
                     /locus_tag="KQD82_003228"
     CDS             337..1266
                     /gene="glsA"
                     /locus_tag="KQD82_003228"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG4381682.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1364..1927
                     /locus_tag="KQD82_003229"
     CDS             1364..1927
                     /locus_tag="KQD82_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381683.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2415..3251
                     /locus_tag="KQD82_003230"
     CDS             2415..3251
                     /locus_tag="KQD82_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG4381684.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3535..4530
                     /locus_tag="KQD82_003231"
     CDS             3535..4530
                     /locus_tag="KQD82_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG4381685.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4787..5191)
                     /locus_tag="KQD82_003232"
     CDS             complement(4787..5191)
                     /locus_tag="KQD82_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG4381686.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6273..7187
                     /gene="queG"
                     /locus_tag="KQD82_003233"
     CDS             6273..7187
                     /gene="queG"
                     /locus_tag="KQD82_003233"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG4381687.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRNIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYIFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7293..9530
                     /locus_tag="KQD82_003234"
     CDS             7293..9530
                     /locus_tag="KQD82_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381688.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9727..>9825
                     /locus_tag="KQD82_003235"
     CDS             9727..>9825
                     /locus_tag="KQD82_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG4381689.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEK"
BASE COUNT         4025 a          863 c         1816 g         3121 t
ORIGIN      
        1 gatagttaga ttactaggtt gttaaattat tgaattgtta gattgttgaa ttactaaatt
       61 attaggttgt taagttactg gattattaga tagttagatt attagattgc taaattatta
      121 ggttgttaaa ttactggatt attagattac taaattgcta ggttgttaaa ttattgaatt
      181 actaaattat taagctcttt aaactattta aattgtttga aaaatgaatt gattcttgta
      241 gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat aaacttgtgc
      301 taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga tgagacatta
      361 ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt cgctagttac
      421 ataccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat agatagcgaa
      481 aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag tatttcaaaa
      541 ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt ttcaaacgtt
      601 ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat taatgacaca
      661 aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc ccttataaat
      721 gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa gttagcaaaa
      781 aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac tggagataga
      841 aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa tgtagaagat
      901 gttctagacc tttattttaa acaatgctca attgagatag attctgtaga tttagcgaga
      961 attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca tttaatgagt
     1021 gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta tgatgcttct
     1081 ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg tggtataatg
     1141 gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga caaaaaaggt
     1201 aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa attaaatatt
     1261 ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata tataattaaa
     1321 ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag aggtattggt
     1381 attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag atattctaaa
     1441 agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg aagaacctca
     1501 tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag aattagttga
     1561 ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa aaaatgtggt
     1621 tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag aagaattgta
     1681 catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg aagatgtttt
     1741 agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg aattagttga
     1801 acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac aacacaatgt
     1861 tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg aggaatgtcc
     1921 tagttagaga attaattatg gattaattaa agatataata ttagagattg aacatcaaaa
     1981 tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt ttaatctcta
     2041 tttttttgtt ttcttatacc tattgtattt gtaaataata tgtatagtat aagatgtata
     2101 cactatctga gaagtgctta gataatatac aaagtatgta agattataat atttgaaatt
     2161 gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata taaattaaat
     2221 caatgctaga aatatgttaa aagaggaatg agattagttg ggatagaaca tcccttagag
     2281 cttatgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc atatgttggc
     2341 taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag tagtacaaat
     2401 aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa ggatttgcta
     2461 atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt agtagaaatc
     2521 caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt caagaaatta
     2581 gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg aatctgggtg
     2641 gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat ataagaagga
     2701 tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta ggtggtggtg
     2761 tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag ggtgatgaaa
     2821 atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt tttaaatttg
     2881 agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt gtgtgtcttg
     2941 atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat ggggttttgg
     3001 aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta aatgatagta
     3061 tgatgccatt tggagacaaa aaagacagac atgctcctat aggggaagga aagataggat
     3121 taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca tttttcttag
     3181 aaactccttt tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa gaaatactat
     3241 ctagtaaata aacctttatt aaaaatatgt gaaatactcc gccctcaaca taattagggt
     3301 ggagatacaa ttatcaaatg tgataattta aattaatgga taggaataac aaaaattgta
     3361 tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat aaaatataat
     3421 tatattatga atagtatgta aatatattgc aaagattctc ataatttgtt gtataattaa
     3481 agatgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa agatatgaga
     3541 aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat tccaatacaa
     3601 aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca aggaataaac
     3661 tttattgata gtgcaagagg gtatacaata agtgaggaag caatcggaat tgccattgaa
     3721 ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta tgattcaatg
     3781 aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga tttatatcaa
     3841 ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat ggcttatagt
     3901 gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac tagtcacaat
     3961 ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca attcccttac
     4021 aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa aggaattggg
     4081 ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact ggctattaaa
     4141 tatatactat ctaaagatta catagatgta gttataccag gtatggagag tgttgaacag
     4201 gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga taataaggaa
     4261 attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga atattgtatg
     4321 ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta ttatactaga
     4381 tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa acctactgag
     4441 tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat aagagaaatg
     4501 cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg tgtattaaat
     4561 ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat ttacatagtt
     4621 atagctatta tataaattta atatttgttt aatacttatt aaatatttac ttaatgcttt
     4681 ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat tagtatagtt
     4741 tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat ttttcaaaaa
     4801 tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc ctagggttat
     4861 ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt tttattattc
     4921 cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc cccattctct
     4981 tatttgggaa gaataatttc aagactgtta ttagattttc ttcaacagtt tccccttgat
     5041 actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta gaatattttc
     5101 cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta gatttactaa
     5161 taggtctacc tggaattgtt attttaatca aatttttcac catccttata cagtatttat
     5221 tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac agagcttgtt
     5281 tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt acaaatttta
     5341 aaatttgata tatgatggac atattcatat tagactatta taagaccttc ataatattaa
     5401 taagtatagt ttagtaaaga gagtaaaaag aaatccatat aagtcaagta aagacttaat
     5461 aaatggcaat gtaaatggga tattaattat aagaaaaaat caatcttttt gcattaacta
     5521 taggaagaat gtcaaaatat aactaatgat atttgaagtt ttagagttta tattaaaagg
     5581 taagaaggtt aagtgggtaa aaaaggttga atagattaaa aaggctaagt atctaaaaga
     5641 gttaagtagg taaaaaagtt aaataagtaa atgaaaaagg ctaagtatat aaaagagtta
     5701 ataggtaaaa aagttaaata agtaaatgaa aaaggctaag tatataaagg agttagtaga
     5761 taaaaaaatt aaatagataa aaagattaag tatctaaaag cgttggtaga taaaaaaatt
     5821 aaataaataa aaaagattaa gtattcaaaa gagttagtag gtaaaataat taaatagata
     5881 aaaagactaa ttatttaaag gagttaagta gatagaaaag ttaaataagt aaatgaaaaa
     5941 gacgaagtat ttaaaagagt tagtaaataa aaaagatgaa gtatctaaaa gagttagtag
     6001 gtaaaaaact aaataggtaa aaagatgaag tatctaaaag agttagtagg taaaaaatta
     6061 aataggtaaa aagacgaagt acctaaaaga gttagtaggt aaaaaaatta aatagataaa
     6121 aagacgaagt atctaaaaga gttaagcagg taagaaaatt gaatagctaa aaagattaag
     6181 tatttaaaag agttaatagc tagaaaaggt taaaaagact aaaaaaaata aataaattta
     6241 aaaagtcaat tagattaaaa aggaataatt ttatggataa taaggaatta aaagcatttt
     6301 gtaatttaat aggattaaac tgtgtgggag tagcaggggt agataagtat gataatttag
     6361 aaaagatact taaggacaga caacaaaaag gttatttaac tggtatggaa gaaccaataa
     6421 ttgagaacag aattaaccca agaaatataa tgaaggatgc taactctata atagtctgtg
     6481 catttccata tcatataaat agacaagaaa agaataaaaa ttcaaattta tcaaaatatt
     6541 gtcatggaaa ggattatcac attgtagtaa aggacttttt acaacaaatt tgtgattata
     6601 tttctaaaac agtaacagat tttaaataca agttatttgc tgataatgga ccattagttg
     6661 acaggtattt agcttattta tcaggaatag gatattttgg aataaataat aatatcataa
     6721 ctgatgaata tggttcttac atatttatag ggtatatagt aaataattat aaatttgaag
     6781 aagataaacc actagaaaaa agttgtttta aatgtggtaa gtgtgtaaaa tattgtccgg
     6841 gaaatgctat attgggaaat tatgatatga accccaaaaa atgtttatca tatataactc
     6901 aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt aaagaatggc aaaaaggtat
     6961 ttggatgcga tatttgtcaa gaagtatgtc cacataatgt tgaaatacca acaactcata
     7021 ttttggaatt taaagaaaat ataatagatt atttggatta tgatgagata caaaatattt
     7081 caaataaaga atttaaaagg agatatggag atagagcttt tagctggaga gggagaaata
     7141 taataaagag aaatatggaa ataattttag ataagtatga tgactaataa taagagtctg
     7201 tttcatatac tttaatgtgt atttacttta acacttgttg tataaaagtc atattatatc
     7261 attaaagtta aatatgggga ggtgggggaa tattgaaata taataataca tctgaaataa
     7321 atccactaga ttatatattg ggtcaagaaa gagctgtaga agctatggaa ttagggttga
     7381 aaataaataa tccagcgtac aacatatata tagctggtga acctggaact ggtaaaagta
     7441 cttatgcctt aaaggtttta aatgagtatg catccaaaaa aaatacgcat aaagattggt
     7501 gttatatata taattttgaa aaccctagag agcctattat agttgaatta gaaaaggggt
     7561 ttggaagaga acttaagaaa gatatggaga aattaattga aagtctttta gaagatttta
     7621 aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa taaacttctg gatgaatatg
     7681 aaatagaaaa agatatgctt ttgaagcaaa ttaaaaaata tggagaagaa aaaggattta
     7741 aattaaaaca gagtaaaatg ggaatagttt ttatcccttt gaaagaagag gaagatgaaa
     7801 gtgatgagtc tgatgaagag ttttacaaag ctaaaagaga actagaaaat atggcgatac
     7861 aggtcgtata taaaattaga aacttagaag aaatagctga aaaagcagtg ttagagcttg
     7921 aagaagagat agctaaactt gtagtagaac ctcatatcaa gagattgtgt gaaaaatatg
     7981 aaaattatga taagattcaa acttatctgg aaaatataaa gaaagatata atagaatata
     8041 tgtatttatt ctatttagat gaagaggagt taaaagataa gtatgataaa gagcatttta
     8101 ttaaatataa aattaaccta tttgtagaca atggaagctc aaagaataaa gaatcagcac
     8161 ctgttgtagt agagataaac ccaagtccag caaatctatt tggaaaggca gagtatgatt
     8221 atgcaaatgg aaatataaag actgatttca caaaactatt atcaggagca tttcataggg
     8281 ctaatggtgg ttatttagta ctttatgcag accaattact aaaatataca atgtcttggg
     8341 aaattcttaa aaagactttg cagactaaaa aagtaatatt ggaaacacaa acagcaatta
     8401 aaccagaaaa tatgccattg gatgttaaat tagtactgat tggaagccac tatatatatg
     8461 atattttata tagatatgat gaagattttg aaaaatattt taaagtattt gtagactttg
     8521 atagtgaaat ggataaaaat gaagataatg aagaaggaat agcaagattt atagcatacc
     8581 aatgtgataa gaataattta aggcatttta ctaaatcagc agttgaagag gttattaaat
     8641 ttagcactag attatctggt gatttagaaa aactatcaac taagtttaat aaaataatgg
     8701 aagttgtaat agaaggaagt gcttatgcag agtttagaaa ttcggagtat acagagcagt
     8761 gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat caatagagtg gaaacacata
     8821 tggatgaatc aattgaaaat ggatttactc taatagaaac agagggaaga aaagtaggag
     8881 tgataaatgg tttgtcagtc ttaagtacag gagaatactc ttttggtaga gcttcaagga
     8941 ttacagcgac cactagccca ggaagtaaag gcatagtaaa tatagaaaga gaagtaaata
     9001 tgagtggctc tatacataac aaaggagtac ttatattagg tggctatctt tctgagaatt
     9061 ttgcacagga tttactattg tcactaaatg catatgtttg ttttgaacaa aattatggtg
     9121 gaatagatgg agatagtgca tcttctgcag aattatatgt actattatca tctcttagtg
     9181 gagtatcaat taaacaaaat attgctgtta caggttctat aaatcaaaag ggtgatatac
     9241 aagtagttgg tggtataagt gaaaagatag aaggatttta ttctatatgt aaaactaaag
     9301 ggttaaatgg tgagcaaggc gtcataattc ctagaaaaaa tcaaagaaat ctcgttttat
     9361 cagatgaagt taatgatgca gtgcgagatg gaaaatttaa gatatatact gttgaaagag
     9421 tagaagaggc tatagagata ttgacagatg ttaaatttga agaaataaag aaattggtaa
     9481 aagaaaaatt aatatctttc agcaagatac aaactgtgtc aaaagaataa gtttattata
     9541 gaatttaatg ggtatttatg tataataaaa agcaagaact ttaataggtt tttgcttttt
     9601 ttgtaaatat atataaatta aattcttatt ttgtatgttt tatatattga tgtttatttt
     9661 tttgttgata tgattaaacc atttataaat aggttttata aattagtatt ttaaaggggg
     9721 ataattatga aaaatgtaaa tctaaaagag ataaatgaaa aagatgtaat tttggaagaa
     9781 gagataaata aaaaaggaat ggataagaaa aatataattg agaag
//
LOCUS       DAENCV010000140         9189 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAENCV010000140 DAENCV010000000
VERSION     DAENCV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.142"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(60..251)
                     /locus_tag="KQD82_003236"
     CDS             complement(60..251)
                     /locus_tag="KQD82_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG4381690.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            complement(399..641)
                     /locus_tag="KQD82_003237"
     CDS             complement(399..641)
                     /locus_tag="KQD82_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381691.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            853..2676
                     /gene="typA"
                     /locus_tag="KQD82_003238"
     CDS             853..2676
                     /gene="typA"
                     /locus_tag="KQD82_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG4381692.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2766..3674)
                     /gene="trxB"
                     /locus_tag="KQD82_003239"
     CDS             complement(2766..3674)
                     /gene="trxB"
                     /locus_tag="KQD82_003239"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG4381693.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     misc_binding    4476..4707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4815..6296
                     /locus_tag="KQD82_003240"
     CDS             4815..6296
                     /locus_tag="KQD82_003240"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG4381694.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            6306..7199
                     /locus_tag="KQD82_003241"
     CDS             6306..7199
                     /locus_tag="KQD82_003241"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG4381695.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            7323..8384
                     /locus_tag="KQD82_003242"
     CDS             7323..8384
                     /locus_tag="KQD82_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG4381696.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(8539..9051)
                     /locus_tag="KQD82_003243"
     CDS             complement(8539..9051)
                     /locus_tag="KQD82_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG4381697.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         3301 a         1287 c         1315 g         3286 t
ORIGIN      
        1 ctaaattgtt taatacttat acaatttaga tgctctattt attatatttt atttaaaatt
       61 tattctttac atgcttcaaa aaactctgct tctgtttcct ttatttcaag ctcattgtct
      121 attattccaa cagcttcttt tttaaatgct ctacactgac ctatacaacc agtctttaca
      181 ttttcttctc caaccaaagt ttttagctta tttacatcca catttgaaca gtttggacat
      241 actcttatca tatttaagtt cctcctaatt aatgtctaat taatactttt atacccaaat
      301 ataaacttta taaaagtttt tttaatataa attattacca tatataccat ttatatccta
      361 ttgcgagtat tacatattaa ttttatatat tattaatact acatgtacgg tggtatctgt
      421 tgttttctca ctatttccaa ttccttaatt tctaaagact taatcacatt tactttatag
      481 ttttctttag actttatata caaaacatct tctatctttt taccttcaat aaatttatga
      541 gaccacttaa gaagttcttc aaaattacaa acttcttctt ttacaaaagt atcactttca
      601 cttgcaaaat atttaattat tattttggtt acttccaaca tatatcatcc tttactcttt
      661 tattaatctt tttatctatt aaggttatta tttgtctaaa aaattttttt aaacagaata
      721 attttcagat gcaaaactaa cagttttatc caatttaaac tatattaata caatttaata
      781 tattcctcct tgtacctaat aatatattgt gctataatta ataagttatt ttaatcgaag
      841 ggagcaacaa aaatgtcaca aaaacataaa attataaata tcgcagttat agcccacgtt
      901 gacgctggaa aatcaacatt ggttgatgct ttcctttctc aaagcggtgt ttttagaaag
      961 aacgaagtag ttaaagactg cgttatggac agtaacgacc ttgaaaaaga aagaggcata
     1021 actatatatt caaaaaactg tgcaataaac tatgaagatt acaaaataaa catagttgat
     1081 actccaggac acagtgactt ttcttctgaa gttgaacgtg ttatgaaaac tgttgacaca
     1141 gttattctat tagttgatgc tagtgaaggc ccaatgcctc aaactagatt cgttttacaa
     1201 aaatctttag agttcggttt aaaaccaata ttatttataa acaagattga taaaaaagac
     1261 cagagagcgg aagaagtagt taatgaagtt ttcgatttat ttgtagactt aaatgcaaca
     1321 gatgagcaat gcgaatttcc tataatatat ggtattgcta aacaaggtat tgctaaactt
     1381 gaaatggatg atgatagtga agacctttct cctctattta aaactattgt aaatcacgtt
     1441 gaagcttatc ctaattacga taatgaacct ctacagttcc aaatatcagc tcttgcatat
     1501 gatgattatg taggtagact tggtataggt agaatttaca aaggtactct taaaaacaat
     1561 acacaagttg ctatatgtag agaagattct gttgtatcta aaggaaaagt atcaaaactt
     1621 tctgtgtatg aaggtttaaa acaagttgaa gttgatgaag ctactagtgg agaaatagtt
     1681 gttatagctg gtattcctga tatatctata ggtgaaacta tatgcgattt agatagtcca
     1741 ctacctatgg aaatgataaa aatagaagag cctactcttt ctatgaactt cttagtaaac
     1801 gactctccat tcgttggaaa aagtggaaaa ttcgttacaa ctagacattt gaaagataga
     1861 cttgaaaagg aacttgaagt aaatgttggt cttaaagttg aacctcttga tactactgat
     1921 ggatacaaag tttctggacg tggagagctt cacttatcta tactacttga aaatatgaga
     1981 cgtgaaggtt atgaagttgg tgtttctaaa cctgaagttt taatgcataa agaagatgga
     2041 aaactaatgg aaccaataga aagagttgtt gtaaactgtc ctgaagttta ctctggtact
     2101 attataaatg aattaaacat gagaaaaggt atgatggagt ctatgtcaat agaaggagac
     2161 tatgtaaaaa tagagttctt agcacctact cgtggtcttt taggatatcg aagtgaattt
     2221 ataaacgcta ctcgtggaga aggtacttta gttcgttcat ttgaaaaatt tgaagaattt
     2281 aaaggagaaa taccttcaag aggaaatggt gttttaatag cacaaggacc tggtgttact
     2341 atggggtatt ctcttaatgc cttaagtgat agagctgtaa tgtttgtaga ccctggtgta
     2401 gaagtttacg aaggtatgat aattggtatg aattccagaa aagatgatat ggtagtcaac
     2461 ccttgtaaaa acaagaaaat gagtaacgta cgtgcttctg gttcagatga tgctataaaa
     2521 ttatctcctc ctagaatatt tactcttgaa gaagcactag agtttataga agatgatgaa
     2581 ttagttgaaa ttacacctga ttctataaga cttagaaaaa gattcttaaa tgaacatgat
     2641 agattaagat ataacaaatc tagacaaggt aaataaaacg tacatttacc tctaattaac
     2701 aaaatagcca tatagtactg aaaaattatt tcagtttata tggctatttt tatttgaaat
     2761 ttatattatt caacatttat ataattagct gcttgaacgg ctgcaatagc tccatctgat
     2821 gctgctgtta ttatttgacg taaaaactta gttctactat ctcctgctac atatacacct
     2881 tctactgatg ttgtacaaga ctcatctgaa attatatatc caccttcatc caaatcaaga
     2941 acattttcaa acattttatt attaggttta agacctatag caataaaaat accactaaca
     3001 tctatagttc ttttttcttc agtctttaag ttttttactt ctattttcga tacagtcttt
     3061 tctccttcta ttttttctac accatgactg taaagtatct caatattctc tcttgctttc
     3121 acagatttct ccaacacttc ttcacctcta aaactatctc ttctgtgaat taaatacact
     3181 tttgtacaat tattagctaa aaataaagca tcttctagtg cagtatttcc tccacctact
     3241 atagcaactt ctttatcttt aaaaaaggct ccatcacatg ttgcacagta agatactcct
     3301 ctacctgtaa attcttgttc ccctgcacaa cctaatttac gtctttcaac tccatttgca
     3361 agaataacag cctttgcttt atgttcacct gaagaagttt ttacaacctt tacttttccc
     3421 tctaaattta tctcttcaac ctcatcaaac tgaatatcaa caccttgagc aactgcttga
     3481 ttgtatatat tattagaaaa ttctacccca gaaatctttt gaactgctgg ataattttct
     3541 acctcagagg tacttgcaac ttgacctcca taaatatttt tttcaaatac tgtaacgctt
     3601 aaccctgctc tcatagcata aattgctgaa gttaaacctg ctggtccagc accaattaca
     3661 atgatatcta ccataacgtt tacctctctt tatattatta tttcttatat attattctca
     3721 tcattcttat actttactat tattatccat atatactata aacaaaacat tattttttat
     3781 taattaactt tattttacat atatataaaa aaactgcaaa taaaaattaa cttttattta
     3841 cagttctcac ttatttatat aattcaaaac cttattggtt atcatttaca gtgtcttcta
     3901 atatattgct ttctgatata tatgatagaa ttgaaagtcc atacataacc aaaactaatg
     3961 ctgctgttat aagtactatt cccatattta atacctcctt ttgtttacag tatactatta
     4021 ttatgtgaaa tatttatgaa tttaatttga atatatatgt aatttcacac taacaatttc
     4081 ttcaaattaa acattattaa ctatatactt ttgttgaacc aattatgatt aatagtttaa
     4141 atattagcac tatttactta tatttatatg atataccata taattacatt tttcaaatta
     4201 aagttttgta aacttttttt gtaatatagt gtagtataaa attgtaatat tacagatatt
     4261 taatagttat cttttaatta ctttcattaa aaacagcttc atcactatga aaatctaggt
     4321 ttttaaactt ttaaaaaagt taaaaaaata tttttctaat actcaatttg attacaattt
     4381 ttttacaatt taaaaaatat tttttccaaa tttttatatt aatacttgac tttgtctgtt
     4441 ttataaacta gaataattaa taatattaag aatattgaaa ggcattgatt aggaagagta
     4501 aatctgaatc gattaccaga gaaggaaact actagctgaa atgtttccct aattagaagg
     4561 attgaaaact acctaggagc ttctatccga agttatctta atgattttag taggaataga
     4621 cgtttttatt acttgcgtta aagtatcaag tggatttttt aaaatcaatt tgggtggaac
     4681 cgcgggaaac agattctcgt ccctttttta gggatgaggg tcttttttta ttacctaaat
     4741 tcagttttta aaatattctg tatctaattt aattagctaa ttaaaaattt aaaatatatg
     4801 gaggattata aaaaatgtat tattcaagta cgagaggcac cgaagaaaaa gtaactgcat
     4861 cacaagcaat aataaaagga atatcaagtg atggtggtct ttatgttcca agtgaatttc
     4921 ccaatgtaaa aaatgaactt ataaatttag taaatttaac gtattctcag atagcatttt
     4981 ttgtacttag taaattttta tgtgatttta ctgaagatga aattaaaaat tgtatagaaa
     5041 atgcatatga tgaaaagttt gattgtagta gtatagctcc attaaataaa gtaaatgata
     5101 cttatttctt agaactatat cacggtccaa ctttagcatt taaagatatg gcacttacta
     5161 taatgcctca tcttcttaaa acatctataa agaaagataa tttagagaaa gatgttgtga
     5221 ttcttactgc tacatctgga gatacaggta aagctgcact tgagggattt aaagatatag
     5281 ataaaataaa gataattgta ttcttcccag aagatggtgt aagcccagta caaaaacttc
     5341 aaatgaaaac tcaaactgga aaaaatacat atgtagttgg tataaaagga aattttgatg
     5401 atgcacaatc aggtgtaaag aagatttttt ctgataaaga atttaatcaa aaactattgg
     5461 ataataatta tatattatct tctgcaaatt caattaatat aggaagactt cttccacaag
     5521 ttgcatacta tttttattca tatatgaaat tagttagcaa tagtgagata aatttaaatg
     5581 ataaaataaa ttttgttgtc ccaactggta attttggtaa tattctagct ggatattatg
     5641 ccaaacaaat gggtcttcca ataaacaaat tgatatgtgc atcaaatgat aataatgttc
     5701 tttatgattt ctttaaaact ggtgtatatg ataaaaatag aacattgaaa ttaactactt
     5761 ctccttctat ggatatatta atttcaagta atttagaacg attactgttt gaaatatcaa
     5821 atagaaatac tgatattgta aataagcttt tatcagacct tagtgataaa ggagtctaca
     5881 aaataagtga tgatatggaa aaaaatttag cctcattcta tggagaatat tcaaatgaag
     5941 aagaagttaa aagcacgata aacaatgtgt ttaaaaatta taattatttg atagatactc
     6001 atactgctgt tgcatacaat tgttatgaaa aatataaaaa agaaacttct gataacacaa
     6061 aaactgttat tgtaagtact gcaagtccat tcaagttttc tgaagatgtt ctaaagtcta
     6121 tagattgtga ttttaaagac cttgatgact ttgctataat tgatagatta tcaagtattt
     6181 ctaaaataga tattccaaaa cctattaaaa atcttaaaaa tgcagaaata ctacataaag
     6241 atatttatga aaaagatgaa ttaaaactag ctataaaaaa attcttaaag gtataggtga
     6301 ttattatgtt agagattata gttcctgcaa caagtgctaa tataggtcca ggatttgatt
     6361 gtcttggaat tgcgttaaat atatacaata aattttatgt tgaagaaatt gaaagtggtc
     6421 ttgaaataga aggttgtgaa gatgcttaca aaaacgaaaa caacttagtt tatacatcta
     6481 tgaagtactt ttttgataga gttaaaccag aaaaaatacc tgctggtatc aaaataaaaa
     6541 tccaaagtga agtacctata tgtagaggtc ttggcagtag tgcctcttgt atagtagctg
     6601 gagttatagc tgcaaatgct ctatcaggtg ctaatttaga taaaaatcaa ttactaaata
     6661 tagcttcaga aatagaaggt cacccagata atgttgctcc tgctatttta ggaaatatga
     6721 ttgtatctgt tactgataat gagaacatac attatgatat tataaaaatt cctgaagaac
     6781 ttaaattttg tgcaatgata cctaatttta agttatcaac agaaaaagct agaggtgttt
     6841 taccaaagga aataccatat tctgatgggg tattcaatgt tagtagagtt gctttactaa
     6901 tatctgcctt actaaacaaa aactttgacc tacttaaagt agcttgtcaa gacaaattgc
     6961 atcaagatta tagaggaact ctaattgaga actacaatga tattgttgaa aaatctgaac
     7021 aattaaatag tattggagta tttttaagtg gagctggacc tactataatg tctttaataa
     7081 aagaaaatga tgatagtttt gtggataata tgaaaaatta cctacaaaaa ctaaaatctg
     7141 attgggaaat aaaagagtta tgttgcgatt ctaatggagc tgttttaaat ataatataat
     7201 tatcgtcttc tatacaaaat ataactactc ttttattata atttgtagta taataattag
     7261 gactaggtaa taatattaag tattataatc tagttcttta aacatgttta cattgaggag
     7321 ggattatcat aaaagagtta ataattagag atttagttgc aaaagttcct ataattcaag
     7381 gtggaatggg cgttggtata tctaggtcat cactagctgg tgcagttgct aagcttggtg
     7441 gtgttggtgt aatttctgga gttcaaattg gatatgatga ggaagatttt gaaactaata
     7501 ctataaatgc aaatttaaga gctataaaaa aacatatttc taaggccaaa gaaatctcaa
     7561 atggtggtat tattggtatt aattttatgg tagctatgaa agaatatgaa acatacgtaa
     7621 aggaagctgt taaagctggt gtagacctta taatatctgg agctggatta cctaataagc
     7681 taccatcatt agtaaaggga agtaatgtaa aaatagcacc catagtttca actgcaaaag
     7741 ctgctaatgt aattttaaaa atgtgggata gaaaagagaa aactactgct gacttaatag
     7801 ttgttgaagg tccaaaagct ggtggtcacc ttggatactc taacgaggaa cttgataaca
     7861 tagattctat agactatgat aaggaatttg ttgaaatttt aaaggttgca aatacttatg
     7921 gagaaaaatt tggtagaaac ataccagtag ttgctgctgg tggtataact tctagtagtg
     7981 atgttaaaaa atatatagat atgggtgcaa gtggtgtaca agttggtaca agatttgttg
     8041 ctacttacga atgtgatgcc catgagaact ttaaaatggc atacataaat gctagtgaag
     8101 aagatgttca tatagttaaa agtcctgtag gtcttcctgg acgcgccata agaaacaaat
     8161 ttatagaaga agttaaaata aatcgtccag aaataaagaa gtgctataat tgtttgattc
     8221 cttgtaatcc aaaagaaact ccttactgta tttctcaagc tctaatcaat gcagttaaag
     8281 gagatgttga aaattcactt ttattttgtg gaaatgatgc ctataaaatt gataaattaa
     8341 gtactgttga agatgttatc aatgaattga tttctgaatt ataatttaaa gaaaactaaa
     8401 ttaagatttt atcaagctaa ggagtttaaa tacgttattt attaatcttt ataaacatat
     8461 ttatgtcctt agcttttttg tgtcaattta cttttatgga aattttaatt taattagaaa
     8521 tagtattaac ctttataact aatcttcttt tacaagtaca ttggttattt cagctatata
     8581 taaagtatga taatcttttt gtggatacca tcttccatct ataccttcag caataaaaca
     8641 ttgaggttca agtttctgac tatacatttt tttgcatata atagccattt tagattcttt
     8701 aaaataaggt gtatcattta agtgttctat attaaatcca atctgtgata tcttatcctc
     8761 ttctctacca gatacttttc cacaatatga aagctcttta cgaaaatctt catcaaaaaa
     8821 tgtaagagaa aatttatctg tattatctat aaattcctta gtataacgct gtggtcttat
     8881 tactacaaaa gcaacatctt ttccccacat aactccaagc cctccccagc tagcagtcat
     8941 tgtattcact ttaccttcat tttctgctgt tataagtgtc caatctttac ctatcaatcc
     9001 aaatggatta tttttaatct cttctgccaa aatttcttta aaatcactca tatctctaat
     9061 aacctctcat tctcttatat ttatattcct tttattttcg tgataaatta taaatgaagt
     9121 ctagttttga aaataaaata cttttttaaa tatattattg caaataagta aatatatata
     9181 tacactctt
//
LOCUS       DAENCV010000141         9160 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAENCV010000141 DAENCV010000000
VERSION     DAENCV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.143"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(20..1051)
                     /locus_tag="KQD82_003244"
     CDS             complement(20..1051)
                     /locus_tag="KQD82_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG4381698.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            1239..2129
                     /locus_tag="KQD82_003245"
     CDS             1239..2129
                     /locus_tag="KQD82_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG4381699.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(2181..3107)
                     /locus_tag="KQD82_003246"
     CDS             complement(2181..3107)
                     /locus_tag="KQD82_003246"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG4381700.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(3322..3906)
                     /locus_tag="KQD82_003247"
     CDS             complement(3322..3906)
                     /locus_tag="KQD82_003247"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG4381701.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(3991..4893)
                     /locus_tag="KQD82_003248"
     CDS             complement(3991..4893)
                     /locus_tag="KQD82_003248"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG4381702.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(4886..5578)
                     /locus_tag="KQD82_003249"
     CDS             complement(4886..5578)
                     /locus_tag="KQD82_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG4381703.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(5617..6537)
                     /gene="pyrB"
                     /locus_tag="KQD82_003250"
     CDS             complement(5617..6537)
                     /gene="pyrB"
                     /locus_tag="KQD82_003250"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG4381704.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(6833..7855)
                     /locus_tag="KQD82_003251"
     CDS             complement(6833..7855)
                     /locus_tag="KQD82_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG4381705.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            complement(8326..>9160)
                     /locus_tag="KQD82_003252"
     CDS             complement(8326..>9160)
                     /locus_tag="KQD82_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG4381706.1"
                     /translation="LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDI
                     KLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTV
                     YWFNPFIYIMDKHIDLDCELYCDERVLKNRNSDEKKDYALTILNAMRKGSNTSNKFIA
                     GLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKYDME
                     KLHVFRGSVTLLEFTYADAPENVKKEHKRMYEAMGLIPKDSDKISFGVKYYDKLNNLL
                     R"
BASE COUNT         2947 a         1634 c          970 g         3609 t
ORIGIN      
        1 tacaaatact atttttatac tatataaata tttttattaa aactacagct gatacaactt
       61 gtactgtacc tacaaataaa ccatataaag aaacttcttt cccttgtttc actaaatctt
      121 ttacattaac ctttaatcca attgctgcta aagctattat ttctagtttg ttacttattt
      181 ctttgcacaa cacagatact tcttttggta taatgttcat tgaaaaaagt gcacaagtta
      241 taaagaatcc aattacatac catggtatag tcactttttt cttttttatc tcatcctttt
      301 cttcttcaag aatttcccta ttagattgat ttttcatatg tccaaatact aatacaacaa
      361 caactagaaa tattactctt actatcttaa aaatagtagc taaatctttt acattttctc
      421 caacaattgc tccacttgct acaacctgac caattgattg caaagttcca cctatcattg
      481 ctgaagtttt tacaagttca tggctatata aaaattgaga tattattgga agtaaaaaca
      541 taagaaatat accagtaaca tttacaatag ttactgcaat tcccttttct ttatctgtcg
      601 cattaaccac tggaactgtt gctcctattg cagaagaacc acatactgca tttccacttg
      661 ccattagaaa tctaaagttt tcttcaaatc ctagtttttt acctatataa agtgcagcta
      721 ttattgttat tgtcatttgc aatattataa ataaaattcc tgaaactttt aagtctatta
      781 aagtagatac actgagtgtt gctcctaata gtactattga atatgataac aaatctgtct
      841 ctgaaaattt ataacctttt tgaaatacct tttgatttaa aaataggttt cctacaaaca
      901 tacctaaaaa tatagaaatt gatgctgccc ctaccttagg aattaaaata gaaataaata
      961 tactaatata acctactaat actgatacaa acagtcctgg taatatttcc cttatagttt
     1021 ttaatgtcaa actgcttttt ttctttaaca taatatacct accttataat taatatagtt
     1081 ttcttcatag caatagtata taattgtaat aaccataagt aaaataaata tataatatcg
     1141 taatgattaa tgtttttaat ggttattata tatagtataa ttatctgtag aaagtatata
     1201 aaaaatgtaa aatttcatta ggtaaaggag acaaaaaatt gtttgaagaa ttaaaaacat
     1261 ttgtagctgt tgttgaatac aaaaacttta ctaaagctgg ggaatattta aatctatcac
     1321 aaccaagtgt aagtaaacac ataaaaaatc tagaaaatta ttttaaagtt gtgcttataa
     1381 acaggtctat taaacaaaaa accattttta taacagaaag tggacaaatc ttgtacaaaa
     1441 gagctaaaga aatattaaat cttttaaata ttacttatca tgatgtaagc caagtttcag
     1501 atgctataac tggtcactta aagattggtg caagtttaac tattggagag tatattttac
     1561 caaattttct agcattattt tctaaaaagt atcctgacat agatgtagaa gtttttataa
     1621 aaaatacatc aatcgtatct tctcatgtta aggattatat tttagacatt ggtcttattg
     1681 aaggcacttg ctcttcccct tcatttattc aagaatattt ttttgaagat aagatggttt
     1741 tagcacttcc atacaagagt catttattaa aagattttag ttttgataaa cttcaaaacc
     1801 aaaaatggat agtcagggag gatggttctg gaaccagaga ttatttagat atgtttttaa
     1861 gtgttaaaga aattattcca aaaagtatga tggtttttgg aagtaattat gctgtcaaag
     1921 aatctgttag aaataattta ggaattacaa tagtctctaa tctagtgaca agcttacctg
     1981 ttctaaacaa tgagctgtct gtcatagagc ttggaagcag ctacaacaga catttctcat
     2041 atatatttcc aaaagatata actttatcaa aagcagcaac tattttcatt gaagagttaa
     2101 aaatatttag taacttaaat agtatataac attaataaaa tttaaaatca ataattatca
     2161 atatttctta caaagaccct ttaaaatcta ctttcaatta cttttatcat ataaaagaaa
     2221 gtctgattta taggagtagg tatgttatat ttttttccaa gtttgcagat attcttagaa
     2281 aacatatcta cttctgtaag tctatgagct tccacatctt gaagcattga tgttcttcct
     2341 tcttttgaat gttctagtat tctatgtaat gaatgttcca catcatcttc tgtaagagat
     2401 atgtcttttg cttgtgctat agcaacaact tccctcattg cagattttgc taattctctt
     2461 aaatgctcag aactctgaaa aactccataa ggtgcaccaa gtatagctga agtttgattt
     2521 acaccaatat taaccatgta cttccaccac atatctcgtt gaatatcttt tgataaagta
     2581 tattctatat ttacatcatc aaagacttcg gttattatat tagtttttct atcttcacta
     2641 ttatctttgt taccaaatac aattatccca ttagtagtgt gtataatatt attattgatt
     2701 ttctttgcat ctatattggt aacatatgaa tataccattt tttcaattcc aaatctttct
     2761 cctattatct cttcgctatc aactccattt agcagagaca ttataactgt attttttcca
     2821 actaatccat ctaattcttt tatattttct tttaaattat tatattttag acctataatt
     2881 ataaaatcag cctcttgttt atactcatct ttagttacat aatcaaaatc ataccttttt
     2941 ccatttatta taaatccttc ttctaaatat cttttctttc tttcattatc acaaagaatt
     3001 ttaaaatcat atttagagtt aaaaaattgt tctgcaaaag tagctcctac agcacctact
     3061 ccaaaaaata caaccttttt atgattttca tttaattgat ttttcatact tacccctccc
     3121 taatatataa tacttttacc ttatacttat attgtactgc tactatctct tattttgtaa
     3181 atatattttt aacaaaaaaa acagctaacc ttcatttata aggctaactg ctttttattt
     3241 ttaagttata tgttatatta caaattttta ctctaaaaaa tttacatttt ttaagtaaca
     3301 ctataaataa ttaagttttt attacatccc taattccttg aactccctac ttccaggctt
     3361 aacaactggt aattttcctt ccttacataa aggacactca tcagattcat aaacttgaat
     3421 atcaagtttt atagcactat atataggcat acctatatca tgattagttc tatctgctat
     3481 acatgcaaca cctacaactt ctccacctaa ggcttctaat actctttttg tctccatagt
     3541 tgatttacca gtagttacaa cgtcttcagc aattattatc tttgctcctt ttttaacttc
     3601 aaatcctctt cttaactcca ttgtattgtc ttttctctca gtaaatacag cttcttttcc
     3661 taattgtctt cctaactcat aagaaactat tactccaccc atagctggtc ctactactaa
     3721 gtctatatct aagtctttta tttgttcaac aactgtactt aatacctcag cagcatattc
     3781 tggaaatctt aatacttttg cacattgtac gtatctatta ctgtgttttc ctgaagataa
     3841 taaaaaatgt ccctccaaca atgcatcact tttctttaat atatccacta catctacttt
     3901 actcatatta tttttcctcc atgttaatgt tataaaatta ttgtttattt aatctatatt
     3961 ttttatcttt aaaatactat ttattctacc ttatataatt cctcttattt cgtctaaact
     4021 ttttattcct tctctatcca taaattcatt aattccatct attatttcaa tacctattct
     4081 aggattcata aaatttgcag ttccaacttg gatacaagta gctcctgcca ttataaactc
     4141 tatagcatct gtggctttag ttattccgcc cattcccatt acaggtatat ttacattttt
     4201 acacacttca tgtaccattc tcaaagctat aggttttata gctggtccag ataacccagc
     4261 atatacattt tcaaatactg gtcttctatt ttttatatca attgccattg ctttaaaagt
     4321 attaactaat gaaactccat cagcaccttc ttcttcacaa acttttgcca ttcctacaat
     4381 atcttctgca ttgggtgata attttattac taaaggcagt ttcgttatgt ttcttacctc
     4441 acgaacaact tctcttgcca cttcattttt tataccaaaa gccattccac cagctttgac
     4501 atttgggcaa gatatattta attcaataat gtctataggc ttgtcattta acatttgaac
     4561 ccctaataag tagtcttcta atgtcccacc tccaacatta gctattctga ctaagtctag
     4621 tttactaaaa aaaggtaatt catagtctat aaatccttga actccagggt tttcaagccc
     4681 aacactattc atcataccgg atggtgtctc atgaactctc ataccattgt taccaggttt
     4741 tttatttaaa gtaagaccct tgctacttat accaccaagt ttttgtatat cataaatctc
     4801 attatattct tttccaaatc caaaagttcc agacgccatt attactggat ttttaaattc
     4861 tacacttcca aatttcacac ttaaattacc cattaaaaat cacgtcctct ccccagaaca
     4921 ctggaccatc tttacaagtc tttttatttc ctccattagt tttacacgta catactaaac
     4981 aggcacctac tccacaagcc atatggtttt caagagatac cataattctc gttttagtac
     5041 cttctaccat cttaactaat ttttccatca ttggagttgg accacatgtt aatatataat
     5101 catatttctc aacatcaatt atatctgtta caaaagtatt tcctactgtt gtatagactt
     5161 tattacatac ttgtttatat tcatcttcta atataacatc ttctctaaac ccaagatatg
     5221 catcacaatt ttttatattt ttagcaacca aatatagtgg agctacaccg attcctcctc
     5281 ctactagagc tacctttcca tcaaccttag catatccatt accataaggg ccttctaatt
     5341 ttatattatc atttaccttt aggttactca aaatttgagt tccttctcct actactttat
     5401 aaagaaaagt tatcccttct tcatctatat catgtatact tataggtctt gaaagtactg
     5461 gataagtatc ccatgctctt aacatataga attgtcccat tttaccttca aaattacctt
     5521 ttactttcat tctatacata tcttcgccta tatatatatt ttccagaatt ttatacatcc
     5581 caaaatctcc tttgttttac tattatatga taactattag tcttcattta tacctaaaag
     5641 ttttattatt aaagccattc taacatacat tccatattta gcttgtttaa aatatacagc
     5701 tctatcatca ctatctacat ctatgtctat ttcatttact cttggtaatg gatgcattac
     5761 taacatatct ttagatgcct tttctaattt agctttattt aaaatataat agtttttaag
     5821 tctttcatac tctgatttat cttcaaatct ttcttgttga actctagtca tatataaaac
     5881 atccaagctt cctataacat catccaaatt attagtttca taatatgcat gtccctttat
     5941 agcttctttt atataatctg gcatttttaa ctcttcaggc gctataaata caaactttgt
     6001 atttttatat cttgccatag ccttaaccaa agaatgcaca gttcttccat attttaaatc
     6061 accacataat cctattgtat ggctttctaa agttcctttt aatgatttta tagtaagtaa
     6121 gtctgtaagt gtttgagttg gatgctggtt cttgccgtct cctgcattta taaatggcac
     6181 ttccgtatat ctagctgctt ctgaagctgc acctgattga ggatgtctca tagctattat
     6241 atcaacataa ccagatacta tcctcattgt atctcctaga gtttcacctt ttgatgcaga
     6301 ggatgaattt ggctctgaaa agcctactac acgtcctcct aatctgttca tagcagattc
     6361 aaagcttaat cttgttcttg ttgatggttc ataaaataat gtagctaata gttttccttc
     6421 acatatacga gaatattttg acggattgtc aattattttt tgagctaatt ctaatatttc
     6481 atctatttct tctattgaaa agtcttctgg ttggattaaa tttcttgatt ttaacatctt
     6541 tcgtcctcct tttttagcct ctctgaacta aaattaaaag tatttttgta ctgttagtag
     6601 cttaccacct ttgccctatc ctgtcaaggg attgcgcaaa taatcatcat atgaaaaggc
     6661 tcctatttaa cagtcatatt aacttgaatc ttgattagat ttaaagtgat acaaaaacgc
     6721 tctatagtac atttaaaata taatcatttt ttgtttccat gattaaatta aatataatat
     6781 agagcgttta tatatattta tgtatttatc aatttgtcat attacaaacc gattataata
     6841 ttctttttgc tgtaacaaat cttgctgtat agtatgatga atttatacta gttacactaa
     6901 ctgtttttcc tgttgatggt gaatgtatca tattaccccc acctacatat attccaacat
     6961 gactaacaga accagaacca ttagttgtga agaatactaa gtctcctggt tgtaaatttg
     7021 ctttacttac agtttgtcca tatttagatt gtgctttaga tgttcttggt atattctttc
     7081 ctactgcatt tctatacaca tattgtgtaa atccagaaca gtcaaaagtg tttggtcctt
     7141 ctgctcccca aacatatgga caaccttgct tagaatatgc caagttaagt acagcttgaa
     7201 ctgcattaga gttagctgtt ggcacatttg aattattatt ttgttgatta gtatttgcat
     7261 tgttttgttg attattattt gtagaatcta cattttggtc aatacttggg tttacttgtg
     7321 gctcttctat tacaggagct ttatcagata tataagatga ttttacatat ccttctgttt
     7381 ccccatcttt aacttttaac caatctccat tatcttccaa aaccaataaa gaacttcctt
     7441 tttccagctc ttcaattact tctgaacttt cactcttatc ttttcttaag tttactttat
     7501 cagcgttagt atatcctaca ccttcagata tatctacata tctagtagct aaccaacctt
     7561 cgccatcttc aactttaacc ttaacccatt ctccttgagt tcctaaaacc gtaaattcat
     7621 ctccagaata agctatgttt tgaacttgac cttcttctct tatctttata gctactccat
     7681 cttttatttt tgcaacttta tattttactt ctttatattc acctaaattt aattcagtat
     7741 ttgttttatc atccttattt tgagatgaat catttacttc atctgcattt acaattgaac
     7801 ttactgataa tgccataact gatgcaaata ctggtattaa tatctttttc ttcacgataa
     7861 acctcctaaa attttcccat aattatctta tgattctatt ttgtaggtga agtgtaccta
     7921 aggataaaat actaatacac aatacattaa taaaagctct atctgtaact tttttgtaaa
     7981 ccactttata taacttttat agtattatta tattaataaa ataaataaat tagcttatcc
     8041 aatatgtcac ctaaatatac attatttact taattatata atatgggtct gtaaaagtaa
     8101 agaattttgt accattcgag gttaaagtgc gcataaatat tgttttatgt gtttttttat
     8161 gcgcttttat atggcgtata ttttttgatg aaataactat aaaagttggt tgttttgtga
     8221 aattttttac aattgaatat aattattaat actttcttta ggacttttgt gaatacttac
     8281 ttttaaattc ttttaagttt cagctatttg ctattatata aatatctatc ttaataaatt
     8341 gttcaacttg tcatagtatt ttacaccaaa gcttatttta tcagagtctt taggaatcaa
     8401 tcccattgct tcatacattc ttttatgttc ttttttaaca ttttcaggag catctgcata
     8461 tgtaaattct aaaagtgtaa cacttcctct aaaaacatgt agcttctcca tatcatattt
     8521 aggcaatata ttaagattaa caatactcgt tgaattaacc ttcaaaaaag taatagaaga
     8581 taataaacat aaagttaatg ccattaatat accattcttg tacttttcat taaacatatt
     8641 cataactctc tttttaatat cagattgctt atggagtcca gctataaatt tatttgaagt
     8701 atttgaacct tttctcattg catttagaat tgttaaagca taatcctttt tttcatctga
     8761 atttcgattt tttaaaactc tttcatcaca ataaagttca caatctaaat ctatatgttt
     8821 atccattata tatataaatg gattaaacca atatactgtt tttaaaaaca ggattacata
     8881 ttttaaataa aggtccctac tcttaaaatg cataagctca tgtctaaaaa tccagtctaa
     8941 ctctttcaat ttatactcat gtggtggcaa aagcacacat gaattaaaaa atgctggtgt
     9001 ttcaacttca taagagtatt tgagttttat atcttttgtt atattaaatt cttctaatat
     9061 atttttataa agatttacaa tctctccatc atcaacatca taagataagt catttattaa
     9121 atttttaagt tttatatatt taaatatagt gtaaattaaa
//
LOCUS       DAENCV010000142         9109 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAENCV010000142 DAENCV010000000
VERSION     DAENCV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.144"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            476..3118
                     /gene="adhE"
                     /locus_tag="KQD82_003253"
                     /gene_synonym="adhC"
     CDS             476..3118
                     /gene="adhE"
                     /locus_tag="KQD82_003253"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG4381707.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3478..3819)
                     /locus_tag="KQD82_003254"
     CDS             complement(3478..3819)
                     /locus_tag="KQD82_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381708.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIVNIKNQN
                     EKVILNKSIKK"
     gene            complement(3846..4517)
                     /locus_tag="KQD82_003255"
     CDS             complement(3846..4517)
                     /locus_tag="KQD82_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381709.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4529..7105)
                     /locus_tag="KQD82_003256"
     CDS             complement(4529..7105)
                     /locus_tag="KQD82_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4381710.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKRGQCEIFSNLNESELNNFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYIAIAMSLFAGIVIYSIYVISIYQRVQEYGILR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIMLIISFFTYLKIRRISPIDAIRKTFGSDKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPNGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDSVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGLI
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7313..8248
                     /locus_tag="KQD82_003257"
     CDS             7313..8248
                     /locus_tag="KQD82_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG4381711.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8267..9004
                     /locus_tag="KQD82_003258"
     CDS             8267..9004
                     /locus_tag="KQD82_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381712.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
BASE COUNT         3304 a         1183 c         1350 g         3272 t
ORIGIN      
        1 gagattagaa taattttagt ttatatattt tttaaaacta tacagttgtt ataaaaagaa
       61 aaattgcata caagtcaaaa ataagtgtta ttttataaaa atacgacaaa aattttattt
      121 actacttata caaataacat gcaaataaca tataaaattt aataagtaaa aaaaatgaaa
      181 gatataaatt atcattttag tattttattt agtcgaagta aattattcaa atgaaatata
      241 tctcatttag ataaaaaatt ttagtaaaag taaaaaaaat aatatatttg ttaaaaaata
      301 aattgacaaa atattcagaa aaaaacttct tgacctaaga gagtaaaata aataaaatct
      361 aaggtataga gatgaaatct ataaaattat tttaagtata agttaaaaat atagatgatg
      421 catttaagat ttataaaaat tagagatgca taattatttt agggaggttt ttagtatgga
      481 gaaaaaagaa aaggtagtag aaaaatcaaa tgttgaagta tgtagtcctg aaattgtcaa
      541 cagtgttgaa acattaagaa tgagattaga agaaattaga ttggcacaaa aagaatttgc
      601 aacatttacg caagaacagg tagataagat tttcttagct gcttcaactg cagctaatca
      661 acaaagaata cctctagcaa aaatggcagt ggaagagact ggtatgggta ttgttgaaga
      721 taaggttata aaaaaccatt ttgcctcaga atatatctat aatgcttata aagatactaa
      781 aacttgtggt gtcatagaaa aagatgaagc atttggtttt acaagaattg ctgagccagt
      841 aggtgtttta tcagcagtta taccaacaac taatcctaca tcaactgcaa tattcaaatc
      901 cctaatagca ctaaaaacta gaaatggtat aattttctct cctcatccaa gagctaaaaa
      961 ctgtactata gaagcagcta gagttgtaca tgatgcagca gttaaggcag gagcaccaaa
     1021 aggacttata ggttgggtag atgttccttc gattgaatta actaatgttg tcatggcaga
     1081 ggcagacctt atattagcta ctggtggacc tggaatggtt aaatctgcat actcttcagg
     1141 taaaccagct gtcggagttg ggcctggaaa tgtacctgca ataatagatg agagtgcaga
     1201 tataaaaatg gctgtaagtt caatcttagt atcaaaatca ttcgataatg gtatgatatg
     1261 tgcttccgag caagctgtaa tagtaccaga aaaaatatat gaagaagtta aaaaagaatt
     1321 taaatatcgt ggagcacatt tcctaaataa agaagaaact gaaaaagtag gaaaagtagt
     1381 aataattgat ggttcattaa atgccagaat agttggacaa ccagcacatg taatagccaa
     1441 aatggcggat gtagaagttc caaaaacagc tagaataata attggtgaag tagaatctgt
     1501 tgaattaaat gaaccttttg cacatgaaaa actttctcct gtacttgcaa tgtacaagtc
     1561 taaaagtttt gaagatgcag ttgctaaagc agaaaaatta gttgcagatg gaggctatgg
     1621 tcatacatct tcattatatg cagactctat aaatcatcca gagagagtag aaaagtttgt
     1681 aaatgctatg aagacttgta gagtattagt taatactcca tcatcacaag gtggtatagg
     1741 tgatttatat aactttaaat tggcaccgtc tcttactctt ggttgtggtt cttggggtgg
     1801 aaactcagta tctgaaaacg ttggagtaaa acatttgctt aatatcaaga cagtagctga
     1861 aaggagagag aatatgcttt ggttcagagc accagaaaag gtttatttca aaaaaggatg
     1921 tttaggtgta gcagcaagag aatttaagga tgttatggat aaaaagaaag catttatagt
     1981 aacagattct ttcctatata ataatggata tactaagaaa ttaactgatt tattagatga
     2041 gatgggaatt aagcatacta cattctttga tgtagctcca gacccaacat tagcttgtgc
     2101 aagagaagga gctaaagcta tggctgattt ccaaccagat ttaataatag ctgtaggtgg
     2161 aggaagtgca atggatgctg gtaagataat gtgggtaatg tatgagcatc cagaagtaga
     2221 tttccaagac ttagctatga gatttatgga tataagaaaa agagtatatg tattccctaa
     2281 gatgggtgaa aaagcgtatt ttgctgctat cccaacttca gcaggaactg gttcagaagt
     2341 aactccattt gcagttataa ctgaccaaga ttcaggtgta aaatacccat tagcagatta
     2401 tgaattaatg ccaaacatgg ctataattga tgctgatatg atgatggaga tgccaccaag
     2461 attaacagca gcatcaggtg ttgatgcttt aacacatgca ttagaagcat atgtatctat
     2521 gttaagaact gaaccagctg acggtctagc actacaagca ggtaaaataa tatttgaata
     2581 tttacctcgt gcatataaaa atggaaagaa tgataaagaa gcaagagaaa aaatggcaat
     2641 ggcatctact atggcaggaa tgtcttttgc aaatgccttc ttaggaatat gtcactcttt
     2701 agcacataaa ttaggtgcat tccaccatgt tcaacacggt gttgcaaatg cattattaat
     2761 aaatgaagta atcaagttca attgtgcaga agctccaaat aaaatgggtg cattctcaca
     2821 atacagatat cctgattgca tacaaagata tgctgaattt gcttcttttg caggtattaa
     2881 aggaagtaca gaccaagaaa aagtagataa tcttataaaa gcaatagatg aactaaaggc
     2941 aaaagtaggt cttcctaaga ctataaaaga ggctggagta gaagaatcta aattcttaga
     3001 aagattggat gctatggttg aacaagcttt tgatgaccaa tgtacaggtg ctaatcctag
     3061 atacccattg atgagtgaat taaaagagat atatctaaaa gtttattatg gtaaataata
     3121 tctattgtgc aacataggtt ctattgggtt tgtatataaa ttattagatt tgaatataaa
     3181 tagagtttta ttagagatat gtataaatag ttataaataa tagattataa cttaaattct
     3241 gaatggaaat gataaaaggt cagtaattta aagataattt tgaatatgtg tatttaatca
     3301 attaacttta aaattagtcg gaaaattttt ttattacata taattttaaa gcataatata
     3361 taaaaaactt tatgaaaaaa ttataaaaat attcagaaaa ttctattgac taatttattt
     3421 atcatgatat aataagttta gaaaagaaaa aaattggggc ctattaaggc ccccttacta
     3481 ttttttgata gatttattta aaattacttt ctcattttga ttttttatat ttactataga
     3541 gtcagtataa tccacttcaa agttggcaaa agcagagaat aaatctatat ctcttaacaa
     3601 atgaattgtc atacattgat tgttcttatt tagccttaga atcaatgctc ttacaaatgg
     3661 aaattgtatt ctagaattaa ataagttaga accatcagta tctatatcta agctacgata
     3721 attaatatct ataaatatat ctacatcatc attttcttct cttattattc tgtagtcaaa
     3781 atccaagttt aaatttaaat tacctaaaat caataacatt taaatcctct tttctataaa
     3841 tagttttaga gtgttaattt aagctctcca tctattatag atataattct atctgctttt
     3901 ctagcaatat tttcattatg tgttattatt ataagggttt ggttatattt ttttatagaa
     3961 ctttttagta aatccatgac ttcttctgta gttttactgt ctaaatttcc tgttggttca
     4021 tctgctagga ttatacttgg ttttgcagat aaagcacgtg caatagcaac tctttgctgt
     4081 tgaccaccag ataattggtt gggaagatga tttttccttt ccttaagccc caataaattt
     4141 ataaggtctt caatatattc tttatctatt tttttattgt ctaactttag tggtaattct
     4201 atattttcct ttacatttaa tacaggaatt aagttatact gttgaaagac aaatcctact
     4261 ttttgtcttc tatatcttgc tagttcttta tctgaaaggc aacatatatc tttaccagca
     4321 acttttatag taccattact tggtctctca agaccaccta ctaaatgtag aaatgtactt
     4381 ttcccacttc cacttggccc aattattgca acaaattcac ctttatttat ttttaaattt
     4441 gcattcttta gtgcatgtac agatgtttcc ttttttccat aaatcttacc taagttaata
     4501 gtttccaata taggcataat tattcctcct attctgtaat gtttatagct tcaactatac
     4561 taattttatt tatctttctt gatggaatat atgtagctag aatacctaca attatatttg
     4621 ctacaacaac tagtatatat attttgtaat ctattgaaaa tcccaatcca tatgatacaa
     4681 agccctgcat ataatacatt ttaaattgta ttataagtcc tgacacgata gtaattatac
     4741 ttgagagtac accatataat attccttcat aaagtatcat atttttaaat cctctctcag
     4801 agataccaat agctcttaac attccaaatt cactagttct tgaagttaag ttataactta
     4861 tgttgtttat tatgttaagt acactgatta tgaacattat tgctactatg ccataactat
     4921 aaatcatagt tctagatgtc atcttatcag aattttcttt ttccaccatc atatctactg
     4981 ttgtagttcc aggaacttca cttccaattt tacctaaaag gttatttatt tttttcttgt
     5041 ttgcattttc attaaggtct gcataaacta agttatactt atctacacca gttaattttt
     5101 ttaggtattc gctactcgtt attacatcta cactatcatc tccagagtac ataccattat
     5161 ctgcaaaagg ataacttata atagctccaa ctttaaattc atattctaag tattcataat
     5221 tatcttttag actttcataa agccctgtat ccatcccacc atttggatat ttgactttta
     5281 tagtatcacc tacttttata tctacagctg gtgtcccttc accaggagta aaagtctttg
     5341 tgtatttctg tgttttagaa atttctggta catatacaat agctaaattt tcttttttca
     5401 tttttttcaa gtttatattt ccacttacta agtatttttc taatgacttt atcatctcat
     5461 cattataacc ttttagcttt tgctttagtg tatatttgcc agtagtctta ttttttatga
     5521 acagaggatt tagcttataa taaggagttg aatttatagc ttcgtagtaa tctaatctat
     5581 ttattttatt tacctctatt cttgtgttta agatactagc agttttgact ttgtcaattc
     5641 catctatatt ttttatttta tctatctgac tatcactaaa cccttttata tctttaaaag
     5701 aagtattgtt accagtttca ttgagataaa catttgatag aataaaatct gcattcatat
     5761 atgtttgttc ttgtacggat ttgttttgca catctaaaaa agaatattta taattgtctt
     5821 ttatgacgat tatacctcct atactcatag ataatacaat tatcatgaac ccctttttat
     5881 ttctaaagat atttttcgca gatatagatt ttgttactga catatttaaa gttaattttg
     5941 atattaaata attaactttg gttacatttt tatcagatcc aaaagttttt ctgatagcat
     6001 caataggaga aattcttctt atttttaagt atgtaaagaa acttataatt agcattatta
     6061 gtattgtaca tgcaattgat aagagaatta ttttatctgg aataacaaaa ggtgtaacat
     6121 ttatatttcc ttcaaattga atatttccta cagacctatt aaatatttgt gcacctccca
     6181 tacctataca tatacctata ggcattgcta ttagagcaag tatgaaaagt tcataaagca
     6241 taagtttgaa aatcctaaag ttggtggaac caatagctct taagatgcca tattcctgta
     6301 ctctttggta tattgatata acatagatac tatatattac aataccagca aatagactca
     6361 tagcaattgc aatgtattta cttgaattat ctaaagtacc gtttttcata gtagattcta
     6421 taagcattga gtttcttcct atacttttct catctaacat agcatttttt attatattgt
     6481 ctatgtttgt atttatatca ctgccttcat taaattcaat ataagttgta aaattattca
     6541 gttctgattc attgagatta gaaaaaattt cacattgtcc cctttttttc tctataaatc
     6601 tatcttcaag gattccaaca actttaaaag tttctggatt ttctttttga tataatttaa
     6661 aagtaaggtt ttgatttatc tctggtttta agcccatact atttaaaatc catttctcga
     6721 ctaccacctc attgctagct tttggaaatc gaccttttat aagtttagaa tgactagtca
     6781 aataattttt atttgcatat agtatgttta taggtaattt ttcattaaca tctgtagatg
     6841 catagtaaga tgttataccc agatttttaa tatctttgct agattttatg gactttaatt
     6901 gatttttctt tatattttta aagtatacat ggtcagttcc aagctctctt ttcaatctat
     6961 ctacatctgc ttgctgagca cttcttgaga gggtaccaat accaacaacg agtgatgtag
     7021 caagaattat actcacaaca attgctaaag acctagcttt ataacttttt atatacctta
     7081 gtactatttt caaattatct ttcatattta ccacttcctt tcttaactaa atcaattata
     7141 tatttatcta aaatatatag caaagtaagt attttcaagt tttaagtagt taattgtaca
     7201 aattcatctg tatttttaca agtttaatta atatatatgt aaattttaac tttttacata
     7261 atttttttaa aatgatataa tccctatata aattgtagag gggaggggaa tcatggataa
     7321 caattttgta tttcctatag taatactaac tttgtttata tttagcaatc ttataatgaa
     7381 taagtataaa ttttacaaag aattagacat attaaaaaga ggcatattaa cattttttat
     7441 atcgtgtttt gcattgattt tatttatgga gggcactaaa tatatacaat taaggttagg
     7501 atatagtttt ttacttgaag gagtattaaa tattatactt tttttggttt taattatttt
     7561 aagtttttat acaggcttta aagttataag attgataaat aataataatg aacaggaata
     7621 taaattacaa aaactagata gtaaaataaa tcataaggat aatataatta tcaaattaag
     7681 aagtcaaaaa catgactatt tgaaacattt gcaagtagtg tattctctat taaatactgg
     7741 gctagatgaa gatgccaaag aatatataat gaatatatca aatagcttta aatcaaaaaa
     7801 ttcaaggtat ggaaaagtat cttatttaga tgctatagct tcattaaaat acgaggaatg
     7861 tcttgaaaag gatatatctt ttgatgtcta cattcaagat tttatggata atttagagat
     7921 tgaacctaat gatatgagtt cagtactatt aaacataata gataatgcaa tagagtcttt
     7981 gtctactata aaaaaagaaa agaagtatat aagattacat gcatatgaag atgaatttca
     8041 gtatattata gctataaaaa ataatggtcc taaaatacat aatgttgaaa gtatattttg
     8101 gaatggattt agtactaaag aaggacaaga taggggatat gggctacatt tagttaaaaa
     8161 tatattagat aagtatgatt atagtatact tgtaaatagt gatgattttt taactgaatt
     8221 tgtaatctta atcccaaaat attattagtt aggaggagaa aagtctatga taaaagtttt
     8281 gatttgtgat gatgataaga ttgtaagaga tgatatagca aatatagcta aagagtctgg
     8341 atatgtagat tatgttaaaa aagttaagga tggtatagag gcaattgaat ttataaaaaa
     8401 tgagaaaata gatttattga ttattgacat tgatatgcca tataaaaatg ggattgattc
     8461 tgctgaagaa attctttcta tagataagga tatacatata gtatttgtga cgggtttcgc
     8521 tgattatagt cttgaaagct ttaaagtgca tcctatagat tttgtagtta aaccatttaa
     8581 aaaagaaaaa atacttgatt caattaacat agcaatagac catattaact cccataagat
     8641 tgctaataat aattttatag aagacaccct ttttgtatat aaaataagga agcagataca
     8701 tatgattaat tttgatgata tagttatgtt tgagaaaaat tcaagagcaa ttaatctaca
     8761 cactaaagat gaagatataa taaagtttta tgagaacttt gatgatttaa aaaaacgcct
     8821 acctcctaat ttctttatga cccatagaca atacatagta aatctaagat ttatacataa
     8881 ggttatacct ataaataaat cttcattaga gataaggttt ataaattttc aagaaaatgc
     8941 catattaaac aaatctctag aaaaagattt tttatataga ttttataggg ttaaaagatt
     9001 ctagtgaaga tagaagaaaa atactttttg tgtgatataa taagtaccgt aaagataatg
     9061 tataagagaa gtttatataa aaattatttg taattatgat ttaatctat
//
LOCUS       DAENCV010000143         9033 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAENCV010000143 DAENCV010000000
VERSION     DAENCV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.145"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            209..1231
                     /locus_tag="KQD82_003259"
     CDS             209..1231
                     /locus_tag="KQD82_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4381713.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1291..2298
                     /gene="gap"
                     /locus_tag="KQD82_003260"
     CDS             1291..2298
                     /gene="gap"
                     /locus_tag="KQD82_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG4381714.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2520..3722
                     /locus_tag="KQD82_003261"
     CDS             2520..3722
                     /locus_tag="KQD82_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG4381715.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3748..4491
                     /locus_tag="KQD82_003262"
     CDS             3748..4491
                     /locus_tag="KQD82_003262"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG4381716.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4500..6032
                     /locus_tag="KQD82_003263"
     CDS             4500..6032
                     /locus_tag="KQD82_003263"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG4381717.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQVSMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            6620..7912
                     /gene="eno"
                     /locus_tag="KQD82_003264"
     CDS             6620..7912
                     /gene="eno"
                     /locus_tag="KQD82_003264"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG4381718.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            8517..8741
                     /gene="secG"
                     /locus_tag="KQD82_003265"
     CDS             8517..8741
                     /gene="secG"
                     /locus_tag="KQD82_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG4381719.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT         3580 a          999 c         1745 g         2709 t
ORIGIN      
        1 aatatatata aaaagcctta tataaaaaga aaaaatactg tgatttgaaa atggtggaga
       61 aatattttat aaaataaaaa aataaaaaag tttttgaaaa actattgaaa tctatacgca
      121 aatcctataa aataaaaata tcttgtggga cttaaataaa cactagggac aaaaaaagtc
      181 ccgaaaggaa agcaataagg agtatgttat gaagaactta ttagaaattc aaaagaaatt
      241 gattccacag gcaattgaac ttatggaaag aagatactct attttaagac aaatatcttt
      301 aagtgaacca ataggtagga gaacattatc aaatgtatta gagattagtg aaagaataat
      361 caggtcagaa actgagtttt taaaagaaca aggacttata gacgtagctg tatctgggat
      421 gactatcact aaagaaggtt ctgatttgtt agataaactt aaggacatca tgagtgacat
      481 catgggattg tctaagttac aagatagagt cagagaaaag cttggaataa aaagggtaat
      541 attagttcct ggaagctgtg atgaaaatgg aagtttacta aaagatgtaa ctagatatgg
      601 atgcgaatat ttcttaggca ttttaaaaga tggagatatc gtttcaataa caggaggaag
      661 cactatgttg gagttttcaa acgttattaa aactgataag agatatccaa actctacaat
      721 agttccagct agaggaagta tgggaactga tgtagagatg caatctaaca atatagttgc
      781 ttcaataagt aagaaattac attctaattg caaactttta catataccag atgagttgga
      841 tgaaagtgca atgaaaactc ttagccaagt gccagagatt aagacaactt tggagtatat
      901 taaaagaaca gacattttag tttttagtat aggtagagca gatgtcatgg caaagagaag
      961 aaaacttcca gaagacaagg ttgatgagat attatctaaa aatgcagttg gagaagcatt
     1021 tggatattat ttcaacaaag atggagaaat agtttacaaa ctgaacactg ttggaattga
     1081 tttagaaaca gttaaacaag taaaagaaac tattgcaatc tttgcaggga ctgaaaaagt
     1141 agaagcctta ttagctatat caaatatgca taaaaacatg gttcttgtaa cagatgaaga
     1201 aagtgcttat aagatattgt cattattata aaagtcaaga cctagcacag agctagaaaa
     1261 catacattta ctttatagga ggtagcgaaa atggttaaag tagctataaa tggatttgga
     1321 agaataggaa gattagcatt aagaaaaatg atggagcaac aagataagtt cgaagttgtt
     1381 gcaataaacg acttaacaga cgctaaaatg ttagctcatt tatttaaata tgatacagct
     1441 caaggaagat ttaacggaga aatagaagtt aaagaaggtg cttttgtagt aaacggaaaa
     1501 gaaataaaag taacagcaga aagaaatcct gctgacctac catgggctga attaggagta
     1561 gatatagtat tagaatgtac aggattcttt acatctaaag ataaagctga agctcatata
     1621 caagcagggg ctaagaaagt tgttatatct gcaccagcta ctggagattt aaaaacaata
     1681 gtattcaaca caaacagtga catattagat ggttctgaaa cagttatatc aggagcttct
     1741 tgtactacta actgtttagc tccaatggct aaagtattaa atgataaata cggtatagaa
     1801 aaaggattaa tgactactat acatgcatac actaatgacc aaaatacttt agatggtcct
     1861 catccaaaag gtgatttaag aagagctaga gctgctgctg gaaacatagt tccaaacact
     1921 acaggagctg caaaagcaat aggcttagta ataccttcat taaaaggaaa attagatggg
     1981 gcagcacaaa gagtaccagt tgtaactggt tcaataactg aattagtttg tacattagga
     2041 aaaaatgtta cagttgaaga aataaatgca gctatgaaag aagcttcaaa cgaatctttc
     2101 ggatatactg aagaaatgtt agtatcttct gacataatcg gaataagcta cggttcatta
     2161 tttgacgcta ctcaaacaaa agttatggaa gtagatggaa aacaattagt taaagttgtt
     2221 tcttggtatg ataatgaaat gtcttatact tctcaattaa taagaacttt aggatacttt
     2281 gcacaattag caaaatagtc agttttgaga aaatagaaaa gtagattgac aaagtccgag
     2341 tttgtagttt aatactacgg actcaggact ttttttaaga aaaaatatat aaaatacacc
     2401 taaataatgt tacatactat atataaaaat atgataatat gatatattta atagggtaaa
     2461 agtatgtact atattatact tggcaataaa aaattacttt attaaaagga gagatattta
     2521 tgtcaatgct taacaaaaaa actatagaag atattgatgt gtgcggaaaa aaagtattag
     2581 taagatgtga ctttaatgtg ccattacaag atggtgtaat tactgatgaa aatagattga
     2641 acggagcttt accaactatt caatatttaa tttctaaagg ggcaaaagtt atattatgct
     2701 ctcacttagg aaagccaaaa ggagaagcta aaccagaatt atctttagca ccagtagcaa
     2761 agagattatc tgagatgtta ggaaaagaag ttgtatttgc agcagacgat aatgttgtag
     2821 gagaaaatgc aaagaaagca actgaaaaaa tggaaaacgg agatgtagta ttattagaaa
     2881 atactagata tagaaaagaa gaaactaaaa acgaagagaa tttctctaaa gaactagcat
     2941 cacttgctga aatatttgta aatgatgcat ttggaactgc tcatagagca cattgctcaa
     3001 cagttggtgc aggcgagttt ttacaagaga gagtttgtgg atacttaatt caaaaagaat
     3061 taaaattctt aggagaagct gttgcaaacc ctgtaagacc atttacagct atacttggag
     3121 gagctaaagt ttctgataaa ttagctgtta taaacgaatt attagaaaaa gttgataatc
     3181 taataatagg tggaggaatg gcttatacat tcttaaaagc tcagggttat gaagtaggaa
     3241 cttctttact tgaaatagat aaagttgaat atgctaaaga aatgatggaa aaagctaaga
     3301 ataagggtgt taacttatta ttaccagtag acgttgttat ggcagaccac tttgcaccag
     3361 atgctactcc aatagtaaca gaagatgcta atgtaaaaga agattacatg ggactagata
     3421 tgggacctaa gactatagca aactttgtaa aaactataaa agaatcaaaa actgtagtat
     3481 ggaatgggcc aatgggagta tttgaatttg aaaactttgc aaatggtaca ttatcagtag
     3541 ctagagctat ggcagagttg actgatgcaa ctactgtaat tggcggtgga gatagtgcag
     3601 cagcagttaa tcaattagga tttggtgaca agatgactca cgtatcaaca ggtggaggag
     3661 catcattaga atttttagaa ggtaaagaat taccaggtat tgcagcatta gataataaat
     3721 aaactaatat atagaggaga ttgtaaaatg agaaaaccta taattgcagg aaactggaaa
     3781 atgcataaaa caataaaaga agctttagaa tttgtaaatg aagttaaaga taaagtaaat
     3841 tctgataaag tagaagctgt tatatgtgca ccatttactt tattaaagga tttaaaagaa
     3901 gctactaagg gtacaaatat aaagataggt gctcaaaata tgcactttga agaaaaagga
     3961 gcatttacag gagaagtttc acctcttatg ttaaaagaaa tagatatgga ctatgttgta
     4021 ataggacatt cagaaagaag acaatacttc aatgaaactg atgaaactgt aaacaagaaa
     4081 gttttaaaag cattagaagt aggaatagac ccaatattat gtgttggaga aactttagaa
     4141 caaagagaag ctggaaaaac taaagatgtt tgtaaagttc aagttgaaaa agctttagaa
     4201 aatgttctta aagatgattt agcaaaagta gttgttgcat atgagccaat ctgggctatt
     4261 ggaactggta aaactgcaac tgctgaagat gctaatgacg ttatatctta tataagagaa
     4321 gttataaaag gattatatgg agaattagct aatgaagtta gaatacaata cggtggaagt
     4381 gttaaacctt caaatgtagc cgaaataatg ggtcaaagtg atatagatgg agctttagta
     4441 ggtggagcta gtttagctag taatgattac ctagaccttg ttaatttcta aggagtgaaa
     4501 tgatgaaaaa accagttgct ttaattatta tggacgggtt tggatacaat aaggatgtta
     4561 agggaaatgc aattgctgag tccaaaactc ctaatttaga tagaataaaa aaagagtatc
     4621 caaacacatt gataaatgct agtggtcttg atgttggtct acctgatggt caaatgggaa
     4681 attcagaagt tggacacact aacataggtg caggtagaat agtatatcaa gatttaacta
     4741 gaataacaaa atctataaaa gatggtgact tttttactaa taaagtttta tgtgaagcaa
     4801 tggataatgc aaaagaaaac tctcttcatg taatgggact tttgtctgat ggaggagtac
     4861 attcacatat agaccatctt aaggctataa tcaaaatggc aaaagataaa ggcgtacaaa
     4921 aggtgtatgt tcacgcattt actgatggta gagatactga ccctcaaagt gcattagaat
     4981 atgccaaaga agtacaagtt agtatggatg aaattggagt aggtgaattt gctacagttt
     5041 ctggtagata ctacgctatg gatagagata agagatggga aagagtagaa cttgcataca
     5101 atgccatggt tagaggtatt ggtgaaaaag caaattctat tgaggaagct attcaaaact
     5161 cttatgatga tggaaaaaat gatgagttta taatgccaac agttattatg aaagatgata
     5221 aaccagttgg aagtataaag gaaaatgatt caattatatt cttcaacttt agaccagata
     5281 gagctagaca aataactaga gctttagttt gtgaagagtt tgatggattt aaaagagaag
     5341 atattaaaaa cttctttgta tgcttaacag agtacgatat aactatagag aatgtgcata
     5401 tagcatttgg acctcaatct ttagccaaca ctctaggtga gtatctagct aaaaatggaa
     5461 aaactcaact tagagctgct gaaactgaaa agtatgcaca tgtaactttc ttcttcaatg
     5521 gtggagtaga agagccaaat aaaggtgaag aaagactatt gataccttct ccaaaggttg
     5581 caacttatga tttaaaacca gaaatgtcag catatgaatt gacagataaa gctttagata
     5641 aactaggaga agataagttt gactttatag tattaaactt tgcaaatcct gatatggtag
     5701 gtcatacagg aagtatagaa gcggctataa aggctgttga aactgtagat acttgtgtag
     5761 gaaaattgat agataaaata gtagagttag gtggaagtgc aataataact gctgaccacg
     5821 gaaatgctga atatatgtta gacccagaaa caggaaaaac tgtaacggct cactctatta
     5881 atccagtacc atttattgta gttggtcaag aatatgaaag tgcaaaatta ttggatggag
     5941 gaagattatc tgatatagca ccgactgttt tagatatgat gaagttagaa aagccagaag
     6001 aaatgactgg tcattcactt atttcaaaat agtcgatttt gataagtaga gaataatata
     6061 taaactacaa tataaattta aaaataatgc agtttaggtt tattttgtaa agtttagact
     6121 ttatgacata agatttaaat tttgtaagtt ctggatattg taaggtgtag atgaagatat
     6181 gttttataaa atttacaaga tagacaattt ataaaataaa aaattacaat atagataata
     6241 atgtaaataa tgatataaat aataacaaga tagacaataa caatataaat aataataatt
     6301 aatatatatt atataagcaa aaattttaga atcattagtt gtacatttta tgtttaatgt
     6361 tttaaatcta gttataatat ttgtgtattt aagattggtt ttggatttta gacaagtttt
     6421 tttatattat ataaattaaa atattataaa aaataaaata aaaataggaa cttttacaaa
     6481 tttacccatc aaataaattt gtacctaatt ttcaaaagca actttatgta ctataatata
     6541 gaaagtagat taaaatagta gacggatgtt tacagtaaat gaaaatttaa ctaatagaaa
     6601 atcgaaggga gatatacaaa tgtcagttat tgaattagta tatgctagag aagtattaga
     6661 ctcaagagga aatccaactg tagaagttga agtagtatta gaagatggag caatgggaag
     6721 agcaatagtt ccatcaggag catctacagg agcttttgaa gctgttgaat taagagatgg
     6781 agataaggga agatacttag gaaaaggtgt agaaacagct gttgctaacg taaatgaaat
     6841 aatagcacct gaaatagaag gaatggatgc atttgaccaa ccagctatag atgcaatcat
     6901 gatagaatta gatggaactc caaacaaagg taaattagga gctaatgcta tattaggtgt
     6961 ttcaatggca gtagctagag cagctgctga tgaaataggt ttaccattat tccaatactt
     7021 aggtggagta aatgctaaac aattacctgt tccaatgatg aacatattaa acggtggaga
     7081 gcatgctgat aacaacgttg atgttcaaga atttatgata ttaccagtag gagcttgttg
     7141 tttcaaagaa ggtttaagaa tgggagcaga agtattccat tcattaaaga aagttttagg
     7201 agaaaaagga ttagcttgtg gtgtaggtga cgaaggtgga tttgctccaa acttaggttc
     7261 aaacagagaa gctttagaat taatagttga agctataaca aaagctggat ataagccagg
     7321 tgaagacgtt atgttaggtc ttgacgttgc tgctacagaa atgtacaaca aagaaactaa
     7381 aaaatacgtt ttagcaggag aaggaaaaga gttaacggct gctgaaatgg ttgctttata
     7441 tgaagattgg tcaaacaact tcccaataat aactatagaa gatggtttag acgaagaaga
     7501 ttgggatgga tggaaattat taactgaaaa attaggaaat aaattacaat tagttggtga
     7561 cgacttattt gttactaaca cagagagatt agaaaaagga attgaaaatg gagtagctaa
     7621 ctctatatta gttaaagtta atcaaatagg tacaataact gaaactttag atgctataga
     7681 aatggctaag agagcaggat atactgctgt tatatctcac agatcaggag aaactgaaga
     7741 ttctactata gcagatttag ctgttgcagt aaatgctggt caaataaaaa ctggtgctcc
     7801 atcaagaact gatagagttg ctaagtacaa ccaattatta agaatagaag aaatggttgg
     7861 agagcaagct agatattgtg gattaaaatc tttctataac ttaaaaaaat agttattaaa
     7921 atggttacta aaaaagatta atgaagagag aatataagat atttaagctc atcttataag
     7981 atataaaaga gataagtgtt aattgatttt tcaattcact tatccttttt tatattttat
     8041 tacttaattt tattcataga ttcagaaaaa atatgaataa atgtttctat ctcattttta
     8101 tctaattgca taggaggttt ttattaaaaa ataaaagtga agacatattt tgatttaatc
     8161 ataattgtca ttaaagtatt attgctaaat gtggattatt tattttaaat tatgttgaaa
     8221 ttaaaagtta tatcaatatt ttaaaatgat tgttttattt tggtggctat ttataatata
     8281 tgcaatgaga gtagattatt ttaagtgtta atttatgcat taatttatgt attaatttaa
     8341 atagttttct cttctaagtt ttattcattt ttatattgag aatatagtaa tatattatag
     8401 taacaatata tttttttgta tgttatttat agcataatag tcttgaaatg gctatattat
     8461 tgtgatacac taaagagtaa tgaaatttaa gtaataaaca gataggaggg gcaagaatga
     8521 gcaacatttt aatgggtgtg caagtagtac tagggatttt actagtaatt agcataatgc
     8581 ctcaagattc taagaatgct ttaccttcag aatttggtgg agaaggaagt caagcttact
     8641 ttaaaccaaa aggaaaacaa gcatttcttg ctagagttac aaagataaca gcagtattat
     8701 tttttataaa tgcattggca ttacttatag ttaagaagta gtgatattgt tgaataactg
     8761 gccattattt aattagtgca tttgctagac atttaaaaat taaatttagt ttttaataaa
     8821 agtacatcaa cataaaaagt acgtaaaata aaagctggac taatgatttt atatgattag
     8881 tccagttttt attttataga ttgactattt gttggcgttt attatttgta tttatatatt
     8941 gtttgtattt atatattttt ttcaaactaa aaaatatata gcaacactac tttcaccatg
     9001 tttaatgaga taatatacac aaagttatat tat
//
LOCUS       DAENCV010000144         8934 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAENCV010000144 DAENCV010000000
VERSION     DAENCV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.146"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            136..1341
                     /locus_tag="KQD82_003266"
     CDS             136..1341
                     /locus_tag="KQD82_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG4381720.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1412..1633)
                     /locus_tag="KQD82_003267"
     CDS             complement(1412..1633)
                     /locus_tag="KQD82_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381721.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1774..2373)
                     /gene="recR"
                     /locus_tag="KQD82_003268"
     CDS             complement(1774..2373)
                     /gene="recR"
                     /locus_tag="KQD82_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG4381722.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2386..2736)
                     /locus_tag="KQD82_003269"
     CDS             complement(2386..2736)
                     /locus_tag="KQD82_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG4381723.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2795..4432)
                     /gene="dnaX"
                     /locus_tag="KQD82_003270"
     CDS             complement(2795..4432)
                     /gene="dnaX"
                     /locus_tag="KQD82_003270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG4381724.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4509..4773)
                     /gene="ffs"
                     /locus_tag="KQD82_003271"
     ncRNA           complement(4509..4773)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KQD82_003271"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4811..4902)
                     /locus_tag="KQD82_003272"
     tRNA            complement(4811..4902)
                     /locus_tag="KQD82_003272"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4866..4868),aa:Ser,seq:gga)
     gene            complement(4903..5358)
                     /locus_tag="KQD82_003273"
     CDS             complement(4903..5358)
                     /locus_tag="KQD82_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG4381725.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5679..6950)
                     /gene="serS"
                     /locus_tag="KQD82_003274"
     CDS             complement(5679..6950)
                     /gene="serS"
                     /locus_tag="KQD82_003274"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG4381726.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7076..7281)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7409..8290)
                     /locus_tag="KQD82_003275"
     CDS             complement(7409..8290)
                     /locus_tag="KQD82_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG4381727.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8302..8811)
                     /locus_tag="KQD82_003276"
     CDS             complement(8302..8811)
                     /locus_tag="KQD82_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG4381728.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2859 a         1570 c          964 g         3541 t
ORIGIN      
        1 tttcacttgt aatttttgca atcttgtatt agattaatat taaaaataat ttatcaataa
       61 ccttattcac aaagacagtt tttatatatc aaaatataat ataaatacta tgctacattt
      121 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      181 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      241 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      301 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      361 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      421 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      481 acgcatcctt ttccaatgat tgctctaagt acattgaaaa aaaactttaa tttacataat
      541 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      601 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
      661 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
      721 gaaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
      781 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
      841 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
      901 gatagagact ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
      961 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1021 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1081 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1141 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1201 tataacttgt caatattact aaaagtttta atagatgacc ctagcagatt agaaatgcta
     1261 aaaagaaata ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1321 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1381 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1441 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1501 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1561 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1621 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     1681 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     1741 aaattacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     1801 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     1861 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtagc
     1921 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     1981 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2041 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2101 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2161 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2221 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2281 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2341 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2401 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2461 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2521 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2581 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     2641 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     2701 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     2761 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     2821 tatcttttat ttctaatatc tcttcactca ctatgttctt taatatttcc tcaccaatgt
     2881 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     2941 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3001 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3061 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3121 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3181 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3241 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3301 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3361 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3421 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3481 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3541 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3601 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     3661 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     3721 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     3781 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     3841 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     3901 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     3961 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4021 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4081 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4141 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcgc
     4201 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4261 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4321 gtcctatatt atcactatat atttgattct ttaaagtctt aataatatga tcttgaccaa
     4381 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4441 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4501 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4561 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4621 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     4681 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     4741 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     4801 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     4861 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     4921 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     4981 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5041 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5101 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5161 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5221 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5281 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5341 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5401 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatagctata
     5461 ttttttttaa attagttcta aaataatttt tagatattca tacaagagtg ttttataaaa
     5521 atatttagtg ttttatgatg aaaattctgc tgtgtaaatt aacataaaaa aactatgaaa
     5581 ttaatattta taataatttc atagtttttt tatttattgt ttactcataa tatattctct
     5641 tcatcaaagc atactctaca tatacaatta atataaattt attttataac atcaacaccc
     5701 atataaggtc ttaattcttc tggaactact acagaaccat ctgcctgctg ataattttct
     5761 aatatagcag ccaaacatct tcctatcgct aatccagaac catttaatgt atgaacatat
     5821 tctgcttttg attttttatc ccttttaaat cttattcctg ctcttcttgc ttggaaatct
     5881 tcaaaatttg aacaagaaga tatttctaca tatctattgt agcttggcat ccaaacttct
     5941 aaatcatatt taaatgcagc tgtaaatccc aaatctcctg tacatatttt tacaactcta
     6001 tatggaagac ctaacatttg taacatagtt tcagcatcat gagttaatga ttctaattca
     6061 ttataagaat cttctggttt aacaaattta actaattcaa ctttattaaa ttgatgttgt
     6121 ctaactaaac ctcttgtatc tcttcctgct gaacctgctt cagatctaaa acaaggagta
     6181 tatgcacagt atttaattgg aagctctgca acatctaata tttcattagc atgtatatta
     6241 gtaacaggaa cttcagctgt tggtattaag aaatactcta atccttctat tttaaacata
     6301 tcttcttcaa atttaggaag ttgaccagtc cctataaaac tatttctatt ggccataaat
     6361 ggaggtaaga cttccgtata gccatgctta gctgtatgag tatttaaaaa gaaattcatt
     6421 aaagatcttt ctaatctagc tcctaatccc ctatataaag taaaccttga cccagttatt
     6481 ttccctgctc tttcaaaatc taaaatacct agacctgtac ctatatccca atgcgcttta
     6541 tgctcaaaat caaatttagt aggttctccc caagttctta tttgaacatt gtcttcatct
     6601 gtttcacctt gtggtacctc tggatgagga acatttggta ttctcattaa tgtatattcc
     6661 atttttgctt ctacttcttt tactttctca tcaatacctt ttatcttatc tgataactct
     6721 ttcaatcttg ccttttcttc tgttacatct tttccttctt ttattaattg aggtattttt
     6781 ttagaattta catttaactc atttttcata acttcaactt cttgaagtat ttctcttctt
     6841 ttattatcta attccactac agcatcaagg tcaaattctc tttcacctct tctttccata
     6901 gcttttttga tatcatctag attttctctt attcttttaa tatctaacat attaattcct
     6961 cctaataatt ttgataatat aagttataat tccaacttaa tttacaaaca ttatacttgt
     7021 aaactcttag tttaaacaaa ttttaaaaat aaaaaaagac ctccgtttac cctataaaag
     7081 ggacgaaaat cttttatccg cgttgccacc ctaattgact atactaaaat gtataatcct
     7141 ctcagaccta taacgtaggt aaacgtctag ctttaactag aagctctaga gtagattcaa
     7201 aaaccattgt aatgagtttt caccatccac tcattctctg aatacaatct gttttttact
     7261 attttccttc acagcttttt aactaattgt tttatttgat aatcattata ttatatatat
     7321 taacatcttt caatactatt attcaaattt ttttagaatt ttctttaatt ttttatatta
     7381 aattcatttt ttctatttaa ttaagacctt atcttttatc cattgtattt agattacctg
     7441 tattattaaa taataatgaa gaaggaacaa atggaacttt tatatcattc tcatcaaatt
     7501 tattttttac cttttccatt acatcaaagt atacatccca gtaatcatca gtttttaccc
     7561 aaggtctagc aactatttgc atggtgttat ttcctataga gtttaaacct attataggct
     7621 ttggatcatt taatattctt ttttcgtttg taaaaatttc atttaatata gacttaacta
     7681 catctatatc actatcatat gaaatattat atgaaaaatc tattcttctt ttgttttgat
     7741 gtgtgtaatt aattatatta ttacttgtca gttgaaaatt aggtataatt atagttttat
     7801 tatctggtgt cttaagaaca gtgctaaata tttgtatgtc agaaactgta ccttctacac
     7861 ctgaaccttg aatataatca cctatattaa atggcttaaa aaataaaata atcataccag
     7921 agcctaaatt tgataatatt tctttaaaag ctaaacctat actaaatcca gcagctccta
     7981 acacagctac aactgatgta gtttgtatcc ctataatacc taagcttatc aatgatataa
     8041 tagataacat agatatatat attccatata caataaaatt atttacacct ttactcattc
     8101 catatttgat tagaaacttt gaaaccatcc ttcttactat tttagatata taaataccta
     8161 tcacaaatac aattattgca tatataatat ttggtatatt atcactaaaa ttatcgagca
     8221 tattttctaa agttctctta gttatattgc tatttgttga ggcagtctta gccaatgata
     8281 aaaaattcat tcaactcctc cttatgataa tattccgttg tcctttaact cttttactat
     8341 agcttctata gtttcttctt ttattgcctc aattgtatga atatggctac attctgtcag
     8401 aaccgataat tgtttaaatt catttgtctc aactttcttc ataaagttgt ctatatccat
     8461 tttagaataa atattaagtt ctgcagttat ctgaccatat gttggatgat ttacaatgac
     8521 atcttttata ataccaccta aatctacaat tatctttaat tcatcataaa tttctttaga
     8581 atccttatga tttttacact ttatttgttt tgtaaaatac ggttgattat atattatata
     8641 tccttgaggt gtagctatta tttcaaatcc tctagctctt ataagggcta tatcttgaac
     8701 tattacttgc ctacttacat ttaatagttc agctagtgtt ccacctttta caggatgctt
     8761 agattctttt aaaatatcta ttaatttact tcttctttca ttagaattca tattacacct
     8821 tctttccaaa taaaaacttt acctatacta tataatttta tattttatct tattataaat
     8881 caatattgac tttatattct atttaatctt aacttttata ataaataaaa aaga
//
LOCUS       DAENCV010000145         8918 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAENCV010000145 DAENCV010000000
VERSION     DAENCV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.147"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            102..443
                     /locus_tag="KQD82_003277"
     CDS             102..443
                     /locus_tag="KQD82_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG4381729.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            594..1007
                     /locus_tag="KQD82_003278"
     CDS             594..1007
                     /locus_tag="KQD82_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG4381730.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1209..1850
                     /locus_tag="KQD82_003279"
     CDS             1209..1850
                     /locus_tag="KQD82_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG4381731.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1874..3127
                     /locus_tag="KQD82_003280"
     CDS             1874..3127
                     /locus_tag="KQD82_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG4381732.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3173..3823
                     /locus_tag="KQD82_003281"
     CDS             3173..3823
                     /locus_tag="KQD82_003281"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG4381733.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNAKEVIQIAENSDIDFKDNNIYLDNEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3874..4470
                     /locus_tag="KQD82_003282"
     CDS             3874..4470
                     /locus_tag="KQD82_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG4381734.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4483..5322
                     /locus_tag="KQD82_003283"
     CDS             4483..5322
                     /locus_tag="KQD82_003283"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG4381735.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5567..6721
                     /locus_tag="KQD82_003284"
     CDS             5567..6721
                     /locus_tag="KQD82_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG4381736.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7027..8724
                     /gene="ade"
                     /locus_tag="KQD82_003285"
     CDS             7027..8724
                     /gene="ade"
                     /locus_tag="KQD82_003285"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG4381737.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3647 a          860 c         1579 g         2832 t
ORIGIN      
        1 tttaaatatc ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa
       61 acactaaaat taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta
      121 ttaatgttga atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag
      181 aaaagcaaga gtatgaagta aagatttata ctgaaagtgg taattatgaa gttggagaaa
      241 atgctccttg tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt
      301 atattgtaaa tctagacaag tgtgaatttt atctcagcaa taattgtatt tttgtgaata
      361 ataattttat aagaatttta ggttcagaaa gaaaaaagaa attaaaaaag aaattagaaa
      421 aattatatga ttatgaagat taatattggg aggctgtatt ttatacagcc tctttattta
      481 ttgattgtat tattaataaa ttttaaatta tgataaaata taaaacaagt aacttatttc
      541 agaataaaat actttttaaa agaaagggac gagatatggg agattataat aaaatgattg
      601 aattaaagaa taacttaggt atcagtgaaa gaaagttaaa atatccttgt atctataaac
      661 attttaaagg aaaatattat gctacaatgg gtcttagcaa ggctattgat gatatagaaa
      721 acatatgtga aatatatggt aaagaaaatt taatccaaaa cagaaataaa tataaattag
      781 ttattagaca tactgaaaga gaagaagata tctatgttta tagggattta gatggaaact
      841 tttatcataa aaaagaagaa gatactaatg atttagtttt atacaagacc ttatatgatg
      901 atacaggaat atttgcaaga cctttggata tgtttttaga aaaagtagat acagataagt
      961 atgtcaattc aattcaagaa tacagatttg aagaagtata taaataatat gagagaaata
     1021 tgcttataaa tttagaaaaa ttctttaaca gtacaataat ttgcagaaat acaaatgaga
     1081 tttattgtaa ataatatgtt agattagatt ataaaataga aacaaagtaa agtattatat
     1141 gatataatta agttttgaga agtgataatt tatatttaat aaaaaatagg catatattag
     1201 gaggacatat gggaaatact ttttatatag taagacatgg acaaacggat tggaatatat
     1261 tgggaaaaac acaaggtcat ggtaattctg accttacgcc tcaaggtata gaacaggcaa
     1321 aagaactttc tgaagatatt ggcaagtatt caattgatta tatttttagt agcgaccttg
     1381 ggagagcaat gcaaacagct caaattttag gagataagct caatattgaa gttcaaaaaa
     1441 ctgaggcact tagagaaatg ggattcggag tttgggaagg ccttttaata aaagaaattc
     1501 aaaaagatta ttcagatata tatgcaacat ggagaaatga accacactta gtaaatatac
     1561 cagaaggcga aactcttaag attataaagg aaagagtaga tgcttttata aaggaactaa
     1621 atgaaaaata tgataataaa aatataattc ttgttacaca ttcaattaca ttgagggtta
     1681 tgttgttgtc atttttagaa tcaggtatgg aaaatatata tagaattaag caagataata
     1741 ctgctttaaa tatagtagaa tttaaagatt atggacctgt tatagtaaaa atgaatgata
     1801 caagtcatat aaaaaatcat gtaaagataa ataattctgc actggagtag gaatcaatta
     1861 taaggagaaa tttatgagta aagttatagt agttggtgga ggagcttctg gaatgatggc
     1921 agctttgtca gcttcaaaaa acaataatga agtaatatta gttgagagaa atggagaatt
     1981 aggaagaaaa cttagggcaa ctggtggcgg tagatgcaat tttaccaata atagagagat
     2041 agaagatttt tttgataaag ttgtcagtaa taaaaaattt ctctatagta gtttctatac
     2101 atttacaaat aaagacctta tttcgtattt tgaaagtaga aatttggaat ataagattga
     2161 agaagaaaat gaccacaaag tatacactaa aaatgataaa tctatagaag taatagaagt
     2221 attaaataaa gatttactca atcacaatgt aaagataatg tataataaga aagttataga
     2281 tattataact gaagaaattg ctctaaaaga tgattctaat aaggacaagt caaagtatct
     2341 aataaaaggg attattttag ataatggaga taaaatacta ggagacaagg ttattatatc
     2401 aactggagga gtgagttatt caaagacggg ttcagatggt tcaatgtata agattcttaa
     2461 aaagcatgga catacattaa acaaactata tcctgcttta gttccactta caatagagga
     2521 aaaatggatt aaagatttac aaggaatatc catgaagaat gtggaaatat cttgtaagat
     2581 taagaaaagg aaaatatcaa aatctggaga catgctattt gcccattttg ggataacagg
     2641 gccttgtgtt cttataatgt cttcttatat taacaagatt attgaaaagg aaaaggtaga
     2701 attaaatata gatttcttac caaatttaag tactgatgaa atctctagta taatcagagc
     2761 atttccaaat aaaaatgttc taaataatct taagcaaata ctacctcaaa atttcttaaa
     2821 agagattttt tctctattat ctttagttga taaaaaggca agtgatttgt ctaaggcaga
     2881 tgaaattaga attattgagt atataaaaaa tatgaagcta acttgtaatg ggactacagg
     2941 tataaacact ggtatggtta cttctggtgg tatttcagtt aaagaaatta attcttctac
     3001 tatggaatct aaattagtta agaacttatt ttttacaggt gaagtaattg atatagatgc
     3061 agaaactggt ggatataatt tacaaatagc attttcaact ggatatcttg cgggtataag
     3121 tgtataaata taatttttta ataattaaat atgaataaat agaggtgtat atatgggtaa
     3181 tttagttata gctgtagatg gtccagcagg agctggaaaa agtacaatag ccaagatagt
     3241 agcaaaaaag ctaaatataa attatataga tacaggtgct atgtacagag cagttactta
     3301 taagtgttta aaaagtggta ttgatgtcaa taatgcaaaa gaagttattc aaatagctga
     3361 aaattcggat atagatttta aagataacaa tatatatttg gacaacgaag ttataaatga
     3421 agaaataaga acaatagaag taagcaataa tgtttctaat gtagcaaaaa taaaagaagt
     3481 aagacagttg atggttgaag ttcaacgaaa aataggaatg aaaagttcag taatactaga
     3541 tggtagagat ataggttcat atgttttccc agatgcagat tacaaattct ttttagtagc
     3601 tactccagaa gaaagaggaa atagacgcta taaagaactt tgtaataaag gatataatac
     3661 tactttagaa gaagtaattg aggatataat aagacgagat gaaattgatt caaatagaga
     3721 atttgcacca ttggtaaaag ctaatgatgc actagaaata gatactacag gaaaaactat
     3781 agaagaagtt gtagaagaag ttgtatctaa aataaatctt tagtttttta tgcatcataa
     3841 tgaaaataaa tgtagtgttg aagggagata aacgtgaatt tttatagatt tgtaataaat
     3901 atttttaagg gtttttctaa aatatttttt aaatatgaag ttataggtgc agaaaatatt
     3961 ccagatagag gaaatatagt aatagcatca aatcataaat cgaatcttga ccctatattc
     4021 ttggctgctg ccatagaaaa tagagaaata gctgcaattg caaagaaaga attgtttaaa
     4081 gtaaaaccct tgggatttat attaaaaaaa ttgcatgtta tgcctataaa tagagaaaaa
     4141 ccagatgtat ctacaataaa aactatttta aggtcagtta gagatggtta tgtattagga
     4201 atatttccag agggtactag aataaaagga gattcttttg gtaaggctaa ggcaggtctt
     4261 tctgtattta caataaaaag taaatctaaa gttgttccag tatcaataat atctaaatat
     4321 aaacttttta gtaaagtaat tgtatatatt ggagaaccaa tatcatttga agaacatttt
     4381 aaggaaaaat tgagtaatga tgaccatgaa aggatatcac aagagatttt agaagtgata
     4441 aaacaaaatt actttaaata ttcaaaataa gggggtaaag ttatgaacat aaaaatagca
     4501 aaaaatgctg gcttttgttt tggagtaaaa agagcaatga aaatggcatg ggatgaagtt
     4561 gaaaaaaatg attctggcat atatgcttta ggtcctctta ttcataacaa gcaagcagta
     4621 gcaaaatatg aagaaaaagg tcttaagact gtgaatgaaa ttgatacaat accaaatcat
     4681 gaaaatatga taataaggtc tcatggagtt cctgaaaata tatataagga agccaaagat
     4741 aaaaaattaa aaattgtgga tactacatgt ccatttgtaa agaaaataca tacagtagta
     4801 agtgaatatc ataataaagg ctatgaaata atagttattg gagatatgaa gcatccagaa
     4861 gttataggta taaatggatg gtgtgaaaat tcagctataa taataaagac cttagaacaa
     4921 atggaaaata tggaatttga taactccaaa agatattgtc ttgttgcaca gactacaata
     4981 aatccagagt tgtatataag tatagttaat aaactatcag ataaattaga agaaatagta
     5041 tttaatgata caatttgttc agctacaaaa acaagacaag aatcagctaa agagttagct
     5101 aaagaagttg attgtatgat agttatagga ggtaaacaca gttcaaatac tcaaaagtta
     5161 gtaaaggttt gtgaagactt agtaccaact tttgcaatag agactaaaga tgaattggat
     5221 gtaaatacgc ttaaaaaata taaaaatttg ggtattacag caggggcatc tacacctaat
     5281 tggataatag aagaagttgt aacattcctt gaaaatttat aatttgatag gtaataaaaa
     5341 taggtggaca ttgttataat ttatcataat atccaccttt gatttcatat tacatgtggt
     5401 aaatagataa cctaatatca aaaagtgtgt atcttaagat gaaaaactaa tagtataaga
     5461 tgcgcacttt ttaatattag aaattcaaaa gaaatgttga tattgattat caatagaaaa
     5521 taaaatataa tataattgtt gcaaaattag tctaggaggt aaattaatgg aaatgttgat
     5581 atctgcttct gaagaggcgt ttttacatgt aggttctatg attggatttt ttattttgtt
     5641 atttggatat attaattata aaactagtgg aaattttact aatatcatat caaaaaacag
     5701 aaaatttcaa ccattaatag gtgctttaat tggagctata ccaggttgtg gcggaagctt
     5761 ggcaataatg ccattgtata taaatggaaa attaagcttt ggagcaataa ttgcaagttt
     5821 aatagctagt atgggagatg cagcatttgt acttatttca tcaaatatta aaatgtattt
     5881 ttttgtcact atagttagta caattacagg aataataact ggacaattag tggattactt
     5941 taaactagaa gaaaaattag ggttaaaaaa ccgtagaaaa tcagataaat acgatataag
     6001 taaaaataac tctaaagaaa ataaagaaca acataatcat gatgaaataa ttttagacac
     6061 tctagctaaa tctcatggaa atacaaatag acttgcattt ataataacac atggaaaggg
     6121 atataaaatt tatataggaa ttatagtaat cggttttata tttatgtcac ttgctcactc
     6181 tggacttaat cttcctataa ttgagaagtt acattcattg gaagaggtaa tagcagtgat
     6241 tggaatatta ttttcaataa tttatatgtg gtgttttaaa aaggtattta aaaattctaa
     6301 tcaaaaagag gaagaaaata aaaagatttc tttgcgagaa atgcttatcc attctgttgg
     6361 agaaatatca tttgtaatta catggatttt tattgcatat cttatttacg atttgataat
     6421 attaatttta ggtggagatg aatacttagt taaattagta ttatcaacag gtgtaataag
     6481 tgtatttata ggtgcaggtc ttggacttat tccaggttgt gggatacaaa ttgtgcttat
     6541 gtcattttat ttgaagggaa ctataccact tggtgcagtg atagccaact ctatttctca
     6601 agatggagat gcacttttcc cacttttagc aatggataaa aaatcttctt tatgggctat
     6661 gattattact actataccag caatacttgt aggtatgata gtttatgcat ttttaggtta
     6721 aatataatta tgaataaaga agtattaaaa aaataataaa gcacataaat ttatttataa
     6781 gaatagtgaa gattaaatat aaaaataaaa aattcaatat gaattaaatt attgcttaaa
     6841 tattaatgag aacatgggag ataatattat tcctcatgtt ctctctattt aattaaagga
     6901 attatggaac aaatttcaag tgatactttc tatcaaaact catatataat atatattaat
     6961 aaattaaact ataatacgta atacagccag tatattgata taaaaaactt aggaggagtt
     7021 gtaaaaatgg gtaatttaaa aagacagtgt gatgtttcaa gtggaagaga aaaagctgac
     7081 attgtactaa aaaatgggac aatcataaat gtatttacag aagagttaat aacaggagat
     7141 gtagcaatag taggagacac tatcgttggc attggcgatt acaagggcaa tgtagaaatt
     7201 gattgtagta acaaatatat atcacctggc tttattgatg cacatatgca tattgaatct
     7261 acaatggtaa tgccaattga attatcgaaa aaattattaa aatcaggtac gacaactatc
     7321 atagccgacc cacatgagtt ggtcaatgta aaaggagcta gtgcaataga ttttttatta
     7381 gaatcgacaa aagatatacc acttaatgta tatattatgg tgccttcatc cgtacctgca
     7441 acaagttttg aaactaatgg agttggtaaa tttagtgcaa aagacatgga gagttatgta
     7501 aataatccta gaattttagg tttaggagag gtaatgtgtt ttaatgatgt tataaattct
     7561 gaaaatgaaa tcttggataa actagagctt tttaaaaata aagttgtaga tggacatgca
     7621 cctaatataa atggtaaatc acttcagaca tatgtatgtg caggaataga aaatgaccat
     7681 gaatgtataa cttttgatga agtatatgaa aaattgagag caggattaaa aattttaata
     7741 agagaaggaa gcgcagcaaa aaatttaaag tctatagtaa gtggtatgct aaagcataac
     7801 ttgccaattg aagaatttat gttttgcaca gatgataagc acttagatga tatagaaaaa
     7861 caaggtcaca taagatggaa tataaaatgt gcgattgatt taggtatgga acctgttagg
     7921 gctataaaag ttgcaactta taattctgca aaagcatatg ggttaaggaa aattggagca
     7981 ataggtgcgg gttataaggc agatattgtt gtattgaatg atttagacaa aatggaagtg
     8041 gatagtgtat ataaagatgg taatttggta aacgaagaga tgttttctaa ttacaattat
     8101 gagattaaag ataaagaatt gcttaataca gtcaagttta aatatattaa taaagaaaaa
     8161 atacagttaa aagtaagtga aaaaaattat gtaatggaga ttgtacctta tcaaattttg
     8221 acaaataagg tatatgaatc tttaccatgt gcagatggat attttgttcc aaataaagag
     8281 tattcaaaac tttgtgttgt tgagagacat agaatgactg gaaatgtagc aattgctcca
     8341 ttaaaaggat ttggcataaa aaatggagca attgctacta ctgtggccca tgattctcat
     8401 aatattatag ttgcagggga taatgatgat gatattctag tagctataaa ttacttaaag
     8461 gaaatacaag gtggttatgt aattgtatca aatggaaaag ttcttgcaca cctaagtctt
     8521 caagtagctg gattaattag tacttttaca gctgaagaag tacaagaaat tactgataat
     8581 atgttggaga tagcaagaaa aatgggagtt ccagagtatg tagacccttt tattacactt
     8641 tcttttatgg cacttccagt tgttccacaa ataagactta cagatttagg tctatttgat
     8701 gttgaggaat ttaaatttat atagataata taaaagtaat aataaatgag gctttctcaa
     8761 aatagggata agtctctttt tattgttgaa atgatgaaaa tatatttgat ttcatacaaa
     8821 aaagagcatc tcatgaacta aaaattttat ttttagatat ccttatttta attaatctat
     8881 catttaacct tgttttaagc atagatatct taataaaa
//
LOCUS       DAENCV010000146         8888 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAENCV010000146 DAENCV010000000
VERSION     DAENCV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.148"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(275..838)
                     /locus_tag="KQD82_003286"
     CDS             complement(275..838)
                     /locus_tag="KQD82_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381738.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(997..1710)
                     /locus_tag="KQD82_003287"
     CDS             complement(997..1710)
                     /locus_tag="KQD82_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG4381739.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2002..3666
                     /locus_tag="KQD82_003288"
     CDS             2002..3666
                     /locus_tag="KQD82_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG4381740.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3773..4672)
                     /locus_tag="KQD82_003289"
     CDS             complement(3773..4672)
                     /locus_tag="KQD82_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG4381741.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4699..5337)
                     /locus_tag="KQD82_003290"
     CDS             complement(4699..5337)
                     /locus_tag="KQD82_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG4381742.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5674..6672)
                     /locus_tag="KQD82_003291"
     CDS             complement(5674..6672)
                     /locus_tag="KQD82_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG4381743.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6770..7345)
                     /locus_tag="KQD82_003292"
     CDS             complement(6770..7345)
                     /locus_tag="KQD82_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381744.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            7661..7924
                     /locus_tag="KQD82_003293"
     CDS             7661..7924
                     /locus_tag="KQD82_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381745.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(8361..8738)
                     /locus_tag="KQD82_003294"
     CDS             complement(8361..8738)
                     /locus_tag="KQD82_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4381746.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         3128 a         1358 c          931 g         3471 t
ORIGIN      
        1 aactatattt aacattatac gtgtatgtat aaatactttt tatatactgt ttgtgctatc
       61 catcccgcac ctaaaggagt gggatttttg cactattctg tattatatat atccaaaaat
      121 attataaaaa tacaaatatt agcagaaatt taaaaactga agaaagtatt ctaactaaga
      181 tttacaaatt cttaatccta tcgaagttaa actactatct tcagcctaat tatctagcaa
      241 tattttttta tatgaaaaac tccaaatcaa gtttttaaat gtttgcgatt tttgatacat
      301 aagaatgttg tctagaatta tattctgata ataaaccttc atactctaag gttaagccag
      361 ataaattata aaaatcattt ggcgttactt caaatgcttg tttatttaaa atgtctatct
      421 cacttttttc taacatgtca gcaacaaaat ttgaacatac atatttatat tcagattgtc
      481 tctgaatatt cattagtaaa tatattaatg ccattgtatc gtaatagtaa tcttctctat
      541 aatcactaat taattttata tttttatata gattttcata ttgtaaatta tctacttcta
      601 acgaatatac cctacacaca gtgttttttc ttatagcata taatccttga tttatatttt
      661 cttcaacaaa tcctccaata aatggattcc ttggatttag ccttccaaaa gaatacattg
      721 gttttagatt ttcatttaat gatattgaca catgtgtata cagcttttta cttatattcc
      781 ttattaagta agataatata gttcctgtat atgttgttac aatatagact ttattcatca
      841 tttcccccca atctagtaac taaataatat tatacactta caatttaata tcataagata
      901 ttctaacata ataaatgtgg ttttacaaat tttgatttta aggtacttta atattatata
      961 atataataaa aatataaata taagttttct atatttttaa ccaacagatg ttttcttcaa
     1021 attaatcaga tgatttagta tatcttgaac ctttccagag tcctttaaat atcctaatgc
     1081 cccatttatt tgtgatttta aatctatttc tacttccata tccaaatcaa catttccttt
     1141 ttttatagat atatttttaa gtgttattgg ataaagataa cttttttcca aagtaatctt
     1201 atttccatta acctcaaatg gaactttttt attgtagtta cttaatattt tttctagcat
     1261 tttatcactt agtttaaatt ttccaatttt cacattctca agacttacaa ctaagtttcc
     1321 atcctttaaa gtaggtctca attcaagttc atattgacta tttaccaaac caaatacctt
     1381 atatggtcct gctaccttta ttttaccatt tttcatttca acaaaattat tttcaagttc
     1441 attaattcca tgcttctgca tcaatgtata tattaaattt ttaaactctt cctctgaaac
     1501 agatattttt ccaaccattc taagtggatt ttttactacc tccatagaat ttatatatga
     1561 gccttttgat aaatattctt catctatgat tttattattt tgtgaactaa catcatatct
     1621 ttcttttggt gtcaagatat atacaacaat tcctgcaagt actaaaacaa taacaattaa
     1681 acttaacaaa atctttgcaa cctttcccat aatcgtctcc tttacaaatc tgtcatcaga
     1741 tttaaaataa atttagataa ctaacttcac ataatattct taacaatata catactataa
     1801 tacagtcttt taaaaattaa ttcaaccaat ctaatgtttt gtaaataaac tgtaaaaaca
     1861 tgtttttagt ataaaagtta atattttgca taatactctt aattacacct ttattttttt
     1921 ctaatcttgg attaaaatta atattatgca atacagtgta gtgttctgta ttataatcaa
     1981 attaaatagg aggtatagaa tatgtcaaat gaaacgaact catctaactt tataaagaat
     2041 attattataa acgacttaga aacaggaaaa catgacagta tcataactcg ttttccacct
     2101 gagccaaacg gatatttaca tattggtcat gcaaaaagta tatgtcttaa ttttggatta
     2161 gctaaggagt ttaatggaaa agctaattta agatttgatg atacaaaccc attaaaagag
     2221 gatgttgaat atgtagagtc cataaaagaa gatgtaaagt ggttaggatt tgattggaac
     2281 gagttaaatt ttgcatctaa ctactttgat gaaatgtata agagagcttt aatactaata
     2341 aaaaagggta aagcttatgt atgtgatttg acacaagagg aaatgagaga atatcgtggt
     2401 actctaactg aaccaggaaa agaaagtcct catagaaaca gaactattga agaaaacctt
     2461 gatttatttg aaagaatgaa aaatggtgaa tttaaagatg gtgaaaaaac attaagagcc
     2521 aaaatagata tgtcttctcc aaatataaac cttagagacc caattatata tagaatatct
     2581 cattcaacac accataatac aggagataaa tggtgcatat atcctatgta tgcttttgct
     2641 catccaattg aagatgcaat tgaaggaata actcactcta tatgtacttt agagtttgaa
     2701 gaccaaagac ctctttatga ttggtttgtt aaagaatgtg agatggaaaa tatacctaga
     2761 caaatagaat ttgctagact taatataaat aatactgtca tgagtaaaag gaaattaaaa
     2821 caacttgtag atgagggtat tgttgatggt tgggatgacc ctcgtgttcc tacaatatcc
     2881 ggaatcagac gtaagggata tactgcagaa gcattgcgca atttctgtag tgaaataggg
     2941 gtatcaaaag ttaactctac ggtagatagt caaatgcttg attatttcct aagagaaaac
     3001 ttacaaccaa aggctcctct tactatggga gttttaagac ctttaaaact tattataact
     3061 aattacccag aagataagat tgagatgctt gaaatagaga ataatgctaa agatgaatct
     3121 caaggaaaga gattagttcc tttctctaga gaattgtata tagaacaaga tgattttatg
     3181 gaagaacctg ttaagaaata ttttagattt ttcccaggaa atgaagtccg tttaaaaggt
     3241 gcttattttg taaaatgtac tgatgtaatt aaggatgaaa atggaaatgt agttgaaata
     3301 catggtactt atgaccctga aactaagagt ggctctggct ttacaggacg taaggtaaaa
     3361 tctacaatac attgggttga tgctaaatca gctataccat gtgaatttag attatttgaa
     3421 ccattaattc ttgatgatat acctgaaaat gaaggtaaac atttcttaga acaaataaat
     3481 cctaattcat tggaaatatt acaaggtttt gttgaaccta cacagataaa agatgctaaa
     3541 ccttttgata aattccagtt tgttagaaat ggattcttta gtatagataa taaatataca
     3601 actgatgaaa aatttgtatt taatagaata gttcctctaa aaagttcttt taaaccaggt
     3661 aaatagtctt acaatcaaaa aatgaagact taaaatataa attacttcta ctataaataa
     3721 acttagttag aagtaatttt ttatttgagt cttcatcatt tactttaaat gattaacttt
     3781 tttcactaca gattcccatg acttctattc catcttttat gcattctatg ttaagaggaa
     3841 aatctggtcc tttttctccg tcaatatctg ttataagtga atcttcacat tctatatgca
     3901 cctttttagt tttaaagtat agtatttcat ttccattata ttgatctaaa taatcacctc
     3961 taagcacact tatcaaaact ggtattgatt ttggtattgg cattccttta aaaataatta
     4021 catccaaata tccatcatct atttcagcct tatatgctag atttatattt cctgctgttt
     4081 tgccattaaa aatcaacata agatacatat ctccatcata atacatttcc tcagaagata
     4141 cttttatctt aaatttcctc atatacaatg cttcttctat tccctttata taataagaaa
     4201 ttcttccaaa tgaatttttt aagtctgtgt ttatcctttg tgatacatcc gtaaacatac
     4261 cagcacttgc aacatttata aagtacttat tattgacttt ccctatgtct attttctttg
     4321 gttttgaatt tatgatttca tttatagctt cttttatatc aaatgaaatt tgaagagcat
     4381 ttgaaaaatc atttgcagtt cctgtaggca aaattccaat tggaattttt atgtctaatt
     4441 ctttcattgc atttactact aaatctacag taccatcacc accagctatc aatatgtgtt
     4501 cataagaact atcaacttcc ttaaatgcat tctttacagg ttctttaata gttaatctat
     4561 agggtatcaa taaataattt ttttcttgat ataattctat gatagtgtct aaatttgata
     4621 atatcttttt ttcacctgaa ttaggattgt atataagttt tacttttttc attatttctc
     4681 cttactcatt ttattctttt agtatccaaa atcttcattt aaaaaaatca catgtattct
     4741 ttctggtgtt aattgtcttt ttataagtgt ataactacag catattcttc ctggacttga
     4801 acagtctata catataccag tagtcttaca tccagtattt gtacttaatc ttttacaatt
     4861 ggctggagca gctattgttt ttgttctttt tatagcttca tctacatctt taactatttt
     4921 atttactcct gctataacta ttactttatc aggtccatat atcatagcag caaccctatt
     4981 cccattccca tctaaattgt aaagttctcc atcctctgtt atcgcattag tacttgtaaa
     5041 ataagcatct gcagaaaatg actttctata tatttctttc atttcttctg gtttgatatc
     5101 ttttgcatat ctatcaagaa aattatattt ttctcttata aaatcaatca ctcctgtttc
     5161 aaataaggtc atagaacctc cacatcctac tagagaacct gctttaacta tttcttcaat
     5221 ttttttaaga agctcttctt tgttacttac taaataccct ttcatattat tgttttccaa
     5281 agattttatt gttttttcta ttcttttttc attaagccaa tttaaatttt gatccatttt
     5341 aacttccccc ttatttttaa tatgtactta attatttata aactttattt tctttataag
     5401 tttattttag tacatctaaa aaaaaataca atataaaact taaatagttt tacataattt
     5461 tttccaaaat aacttactca atcaaaaaag gaaagtagat gtactattct actttcccta
     5521 tattgtatgt agtatttatt gcctaaaatc tatatattaa ttatatgttt catattatat
     5581 aatatcacag ttaattaaaa tgtaatacta tcttcttttt aaacatataa attgtattat
     5641 taaatttaat atatccaact tcaataaatc ttattaattt gatgaagaag atgctatagc
     5701 tgcacttgtt gctgccatta cacatgcaat ttgaatagca acaacaatat ttaaaaatat
     5761 attattagtt atagagccta tattattctc atttcgtatc aaatctgcat aaattgaagc
     5821 tacaatagca gaactaatca ttactctatt actttttcct aaagaccaat ttccaaagcc
     5881 tttttcttct ttaagtttat ttgaaacatt ttttatctct tctattattt catcttctct
     5941 ataatctaat aatgaaattg ctcctattat tggatatcca taataattca ttttacaatc
     6001 attttcttta aatgcttttt taagttcaat agtcttttta catttaagca tagttctatc
     6061 atcactaaat aaaagaattt gggataatgc ttgtaaatca tttcctttat aaaaaccatt
     6121 ttcatttaga aattcataac atttttctat gtattctaaa tcttcatcta aattttttga
     6181 atgaattgca ataagtgcta tattacaata gtcatcacta gaagttagaa atggatgttt
     6241 tttcctcata taatcatata ttattttcat ttttgatata catgtaagat aatccatttt
     6301 ctctctattt tcataaacta ttgatgctgt aagtggtaaa tatgtatcgc cccaaaactt
     6361 ttcactttta agttctttgt atatatctaa aatcatctta aaactacttt ctggatttga
     6421 ttcaaacgaa agtagtgttg ccatataaaa catattatat cctctaaaat tagagaataa
     6481 tccagtattt tttttaataa gctctattcg ttcttccact aatctaggac ttattctctt
     6541 gttcttaagg gtaaatgcaa gtgcacaact atgttgtatc aatcccattt gccaagaacc
     6601 tttaacttca tttaatgctt tgtaattttc tattaataag tttacttttc catttataaa
     6661 cgattgttcc atattgccct cctagtaata tatctcattg tactaatagt tttatattta
     6721 ttatatatat attaaatact acaatcaata catattactt caaagaacat cattcttcaa
     6781 caataatgta gccttttttg taatgtcttc ccatattatt gactctatca tagtactctg
     6841 attcactttc attactccat ttgggcacaa atacatctat tgcattagaa ttactaaaat
     6901 acttactaga ccccctatcc ataacagttt ttaattttcc atctacaata atcttagttc
     6961 caaaacttaa gtgattgttt gctacaaaac catcttctaa atcatctcct gtagcagtta
     7021 tcgctccata tccattttct tgattaagac ttgtgtaata actaacttca aaagttatag
     7081 gtactaactt ctttatttta atacctaact gattttgtag tttctccttt tgtactttca
     7141 acttatactc atcactattt ttataatctt ccataccttg attatacttg gaattaaagc
     7201 ttttacctga ctttactata gtatctatat ttactataga ttctttacaa tgatctgcat
     7261 ctatttttgt ttctccattt accaataatg gagttaaaca tacactacat acaacacatg
     7321 taatcaatgc ttctttaagc ttcataaatt ccccccttat tattttgata tattagcata
     7381 tttaatatta tatgtctaca tttagattta aataccacac ccatttgctt taagaagttg
     7441 tcttatattt aattcacctt aaacacttat aaatcctcta tcttagaatc atatagaata
     7501 aagcttttat ctttaaatat tttttattaa tcttttaaaa tctaaaacta ctttatctaa
     7561 ttttctcttt tttagaattt aagaattaaa attctataat tttaaaacat attgaattta
     7621 tttttttgag atgatataat tttcttaaga ggtggttgat atgataaaaa atacaaaaca
     7681 aattacagta gaagttaatg aaattttatt acaagaaatg tctaagataa ctaatgacga
     7741 aaataatatt ttgacagatt tgataaatga atcaattgta aattatattc ttgaaaacaa
     7801 aaaacataat attgaaaata aagatttaga agaagtgatt aaattttaca aaaaagaaaa
     7861 tactgaaaaa cctaaaagtg cagcaagagc tatagctgaa gcaaaaactc atactctgta
     7921 ttaatcaaat aaaaaagttc ctaaaaacta ggaacttttt tatattaata attattaata
     7981 tatctcaata tttatctcaa atctatcaat ataagcatgt ttaatacaat ctattgtata
     8041 cttatcttac cctagaaata atcaaaacaa aaataaaatt atgcccttta tatctaaaac
     8101 aattaattta catgattaat atacaaaaat tctgactttt aaattcgtat acaatatcaa
     8161 actaaaataa tattaatgac aaacccaata taaattaaat tcaaacaggt ttgattttac
     8221 atcaaactta ttgtattaat attaaataac aagacaaaaa tatttataaa aatcacaaat
     8281 tttacataaa actttatatt tgattcattt aactaattat tgtaaaaact atttatttta
     8341 tgataattaa ctacaccaaa ttattcatct tttttaatat ccttaaccca tttttcaaca
     8401 gagttcaatt cttcttcatt tacccccttt ttgaacaagg atttcataaa actctcaata
     8461 gaacttccat gcatattatc taaaaaagtt tttgtttcaa aatgtaaata ctcttcttct
     8521 ccaataagaa ctgtataatg tgtatgtcta tcaatttttc ttgcacttaa gaattgctta
     8581 tttactaatc ttgataaaag tgttagtgtt gtagtttgct tccaattata tagtttttcc
     8641 atagcttcaa tgacatcttt tgatgctact gtaaaatctg aactccaaat aaatttcatg
     8701 acttttaatt ctgctggtgg tatcttagta atagtcatat gattttacac tcctctcaaa
     8761 cttctacata attaaatact atattaatag acttttcgag tcaaaggttt tatttaaaat
     8821 atctcatcca aatttattaa tacaaaaaat ttatattttt attcaaaatt aaacacacaa
     8881 tctattaa
//
LOCUS       DAENCV010000147         8738 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAENCV010000147 DAENCV010000000
VERSION     DAENCV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.149"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            138..905
                     /locus_tag="KQD82_003295"
     CDS             138..905
                     /locus_tag="KQD82_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG4381747.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1188..1385
                     /locus_tag="KQD82_003296"
     CDS             1188..1385
                     /locus_tag="KQD82_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381748.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1414..2490
                     /gene="vorB"
                     /locus_tag="KQD82_003297"
     CDS             1414..2490
                     /gene="vorB"
                     /locus_tag="KQD82_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG4381749.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2493..3212
                     /locus_tag="KQD82_003298"
     CDS             2493..3212
                     /locus_tag="KQD82_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG4381750.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3225..3764
                     /locus_tag="KQD82_003299"
     CDS             3225..3764
                     /locus_tag="KQD82_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG4381751.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3921..4979
                     /gene="buk"
                     /locus_tag="KQD82_003300"
     CDS             3921..4979
                     /gene="buk"
                     /locus_tag="KQD82_003300"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG4381752.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4997..5890
                     /locus_tag="KQD82_003301"
     CDS             4997..5890
                     /locus_tag="KQD82_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG4381753.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNVLL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5920..7128
                     /locus_tag="KQD82_003302"
     CDS             5920..7128
                     /locus_tag="KQD82_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG4381754.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7152..8411
                     /locus_tag="KQD82_003303"
     CDS             7152..8411
                     /locus_tag="KQD82_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4381755.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3394 a          967 c         1736 g         2641 t
ORIGIN      
        1 ttattttaca aattaagtat aatatttttg ttttataaaa tataattaag ataaatagat
       61 aattttgtaa ttttaatata taatatttag tagagtgtgg gagtagcact taatatttgc
      121 aggaggagtt tttgtatatg ggagaaataa taaatgcatt agatagagcc ctagacataa
      181 ttttgctgtt atatcatgaa aagagagaaa tgggaataac tgaaataagc aaggctatgg
      241 gagtatataa aagtactgta catagaactt tggttactct tgaaaacaaa ggttttgtta
      301 tacaaaatgc agagaatggt aagtattggc taggaataaa tttatatgca ataggtatgg
      361 ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa aaattaaatc
      421 aagaatttaa cgaagtagtt aatgtatcta tattagaaga aagagctcaa gattctccaa
      481 gaagtatcat aatacataaa gagtatggtt caaatcaatt actttcagta aatccatccg
      541 ttggttcaag tagtgaatgt tattgttcag cagttggtaa atgtcttatg gcatttaatg
      601 attctataga ttttgaaaaa tatagaaaaa caccaataca caagtataca gaacatacaa
      661 ttgataattg ggatgatatg atgctttttt tagcaaaaat aaaagaacaa ggatatgcta
      721 tagatgatga agaactagaa catggactaa cttgtattgg agcacctatc ttagataaaa
      781 ataataaagc tattgcagcc ataagtttgt caggtccaac tataagaatg agagaagggg
      841 actttgagta taagattaaa agagttatag aaactgcaaa aagtatatct gaacttttta
      901 gatgattttt aatcttgata ataaaaatag gctatattta aattgttatt tgtaaatatc
      961 aatttaaatg tagcctattt ttctttaatc taaaattaaa tattcttaaa atttaaaatg
     1021 ttattgaaat atataagcga atgtgttatc ttttaattaa gcaataaaaa tagaacaagg
     1081 ttctatacta tagaacaaaa ttgatgcata tatagtattt atacaaaata taaatattat
     1141 atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg gaaaaagtca
     1201 agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgt aaaaaagatg
     1261 caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa gtcgatgaag
     1321 aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgatttagta tttgagatat
     1381 tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat taaaggaaat
     1441 gaagctttag cagaaggtgc tatacgtggt ggctgcagat tttatgcagg ttatccaata
     1501 acaccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt tggaggagta
     1561 tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc aagtgcactt
     1621 ggagcaagag ctatgacttc ttcatctggt ccaggatttt cattaaaaca agaaggtatt
     1681 gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtgcaaag atatggtata
     1741 ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa aggcggtgga
     1801 aatggagatt atcatttaat cgtgttagca cctaatagtg ttcaagaaaa tatggatatg
     1861 gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc cagtacttat actttctgat
     1921 gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga atacgatatg
     1981 gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa agtaataact
     2041 gatataacat attttgaaga ccaagataca tggcaaaaag ggtatataga aaaaatgaga
     2101 aaagttcaag aagatgaaca aagatgggaa gagatagaaa tagaagatgc tgaagtggta
     2161 tttgtggcat acggtatttc atcaagaata gcagaaggtg cagttagaga tgctagagca
     2221 aaaggaatga aattaggtct aataagacca aaaacattgt ggccttaccc aagaaaagca
     2281 tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa catgatggca
     2341 cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata ttcagtagca
     2401 acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca gattatagat
     2461 ggaaaagcta agcaagagga ggtattttaa ttatggctaa aaaagcacca gcaataatga
     2521 tgtgtgaaaa tatgttctgt ggaggctgtg gacactctat agtcgataga attattggag
     2581 aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat ataggttgta
     2641 atcatatgtt tcaatttagt atgaatgtag atgttgtcgt tccacctcat ggtaaaatgg
     2701 gagcagcaat ggcagctatg aaaagagtta gaccagataa atatatattt acaatagctg
     2761 gagatggtgg agcttatgca atagggcttg gagaaactat gtcagctgct actagaaatg
     2821 ataatgtaat atttcttgtt atgaacaata atatgtttgc aatgacagga gggcaaatgg
     2881 ctccaactac tatagaaggt caatatacag cttctactcc agcgggtaga aattacgcag
     2941 atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata gcatatctag
     3001 caagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac atcaaaaaag
     3061 ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt gtaccatgtc
     3121 caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat gaacttgttc
     3181 caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg aatgaaatta
     3241 tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata atgtatgttg
     3301 catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag atgaggggag
     3361 gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct tgtgtaaaaa
     3421 atccagatat actattggga atgaacagcg tatcagtaga taagtatgaa agtttaatga
     3481 aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga aagtacagag
     3541 aagacattaa tgttataaaa gtacctgtta ctgaaatagc acaaaattta ggcaatgaaa
     3601 aagctgctaa catttgcatg ttaggtgcta tggtaaagag tttggattta tttacaaaag
     3661 aagaatttga aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt aaattcaatt
     3721 ctaaaaatgt agaagcattt gaagctggat acgactacga atgaatattt taaatattta
     3781 aaataattat tgaaatactt atttttatat gttagtattt aattaataaa atatagaacg
     3841 tcgttctgga caataggacg aagtctttag gtcggaatac aagaacggcg ttctgtatag
     3901 tagaactagg gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt acatccacaa
     3961 aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc catacaagag
     4021 aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa caactgatag
     4081 tagatatttt aagtagtgag aatcatagta taaattcttt tgacgcagta gttggtcgtg
     4141 gtggacttat gaaggctata aaaggtggta cctatactgt gagtgaagaa atgattgagg
     4201 atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta ttagctaaaa
     4261 ctatagctga tgaaatagga gtacaatcat ttgtagtaga cccagtttct gtagatgaat
     4321 ttgatgatgt atcgaggatt acaggaatat cagacataga aaaagcaagt tggttacatg
     4381 cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga aaatacgagg
     4441 attataactt tattgtagct catttaggtt caggaatatc gattgtagca cataaacaag
     4501 gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc attttctcca gaaaggagtg
     4561 gtggattact tgcatatcct cttatagaat tgtgttactc cggaaaatat actcatgatg
     4621 aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga ttatttagga acaaaggaca
     4681 tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa ggcaaaactt ataatggatg
     4741 catttattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt tcagggaatg
     4801 tagatagagt tatcttgaca ggagggatat ctcactcaga aatggtagtc aaaggagtag
     4861 cagaaaaagt ttcatatcta gctccaatag agatagtacc aggagagatg gaaatggagg
     4921 cacttgctct aggggcactt agagtactta atggagaaga aatagcaaaa acttattaat
     4981 atttaggaga aagagtatgt taaaagattt taataattta ttagaagagg ctaaaaagct
     5041 tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa gaagtattaa aggctgtgaa
     5101 atcagcaaca gatttaaaca ttataaagcc aattcttgta ggagacaagg ataaaataga
     5161 aagtatatca agagaaattg gattaaaagg tcatgaaata ataggttgtc aaaatcccga
     5221 tgaatcagtt aaaaaagctg tagagatagt taaaaataag gaagctaaag ttcttatgaa
     5281 gggattagta aatacaagtg tctatatgag agctatatta aataaagaaa cgggtcttag
     5341 aacaggaaga ttattaagtt tgcttgcagt atatgaatta cctcaatatc ataaacttct
     5401 ttattgtact gatagtggaa taaatgtatc gccaaactta aatcaaaaaa aagatataat
     5461 gacaaatgta cttcttacta tgaaaagtat aggcatggaa aatcctaaaa ctgcgatact
     5521 tactgcaaat gaaatggtag accctaagat acaatctaca gtggatgcca acctacttgt
     5581 agagatggaa aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa tagcatttga
     5641 tgtagcattt gatgcacatg cagcagaaca taaaggtatt aaaagtaata ttgctggtga
     5701 tgttgattta ttagtatttc caaatataga agcagggaat atattaggaa agtcatggct
     5761 tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc cgataatttt
     5821 aggttcacgt tctgacacag ctgaaataaa aataaattca atagcattag cgtgcctagc
     5881 atcacaataa gaaattatta ttaaggagga aaaagtgtta tgaaatatgc aaaaagaatg
     5941 gatatggtaa aagcttcagc tataagagat tctcaaaaga aaatagctca gaaggtagca
     6001 agtggtggaa ctgttatatc ttttgctgca gggcttcctg acccaaattt atttccaata
     6061 gaagagataa gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt tgccctagca
     6121 tatggaccta ctaaaggtga agatgagttg ttagatctat tagtaaaaag aatgaaagaa
     6181 gaagaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc acaacaaggg
     6241 atagcattaa gtgctatgat tttattagaa aagggagatg tagtagttac agaaaatcca
     6301 agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt aggagtagat
     6361 acagatgaag aaggaatggt aacagatgat ttagacaaat tattatctga aaattctaac
     6421 ataaaggtga tatatgtaat accaacattt caaaacccta caggaaaaac atggtcttta
     6481 caaagaagaa aagattttat ggaagtcgtg aataaatatg atgtcatagt aatagaggat
     6541 aatccttatg gagaaataag atttacacca gaacctctac ctacacttaa atctcttgat
     6601 actaaagata aagtattgta ccttgggtca ttttcaaagg tattatgtcc aggatttaga
     6661 gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt aaaacaaggt
     6721 atagatttac agtcaaatca attttcgcaa gtacaagtaa tagaattttt aaagaaatac
     6781 aacttaaatg aacatataga aaaaataaga gcagagtata agaaaagatg tctgctaatg
     6841 ttagatgcta tgaagaaaca tttcccagag gaagctaaat atacagaacc agatggagga
     6901 atgtttatat gggttgaact tcctgaaagt ataaatgtag atgatttatt agataaagcc
     6961 attgatgctg gagttgctta tgtttctgga gagtccttct ttgcaaataa tggtccaaaa
     7021 aatactatga gacttaacta tacaacaatg tcagaagaac aaatcgttaa aggtgttgag
     7081 atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat ataaaaagtg
     7141 agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta atgtttcaac
     7201 ttgttattat agcaatatct tgggaattag tttatattat accatttatc caatatacat
     7261 tatatgatcc aatcctaaaa gcattagaat gtacaaacac tcaactaggt tttctactta
     7321 caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta gcagatagat
     7381 ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta tcatttttat
     7441 ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtaggttgt gcaatcacat
     7501 cattagttat gaactatcct tctatggtaa aaatacttag agttatagga aaagataatc
     7561 aaggtaaggt ttatggattt aatgaagcta tggttggagt ttctggagta gtaatgggag
     7621 caatattctt atatatttat acatgctttg caactcctac attgggtatg agatgggtaa
     7681 tgatatcttt aggaatatta tcaatagcaa tgtgtccagt attatggttt gtaattaaag
     7741 atgtagatat taaagaagaa agagaagaag caggtgaaaa aatgtctgct ggtgatttta
     7801 taacagtact aaaatcacca aatacttggc tagtaggaat aagtatattc tgtgtatatt
     7861 cattcacagt tacaatgtct tattttacac catatataac atctgtactt ggaggttctg
     7921 tagcattatc tggtgcatta gcaataataa gacagcatgg tctaaaatta tttggagcac
     7981 catttggagg atattgtgca gacaaattaa aatctcctac aaaagtactt ttaccaatat
     8041 atgtatttgg tatagcagta atagttttat tcctagtatt acctgcatca actccaatga
     8101 ctatattcat agctttaaca tttgtagtag gtatattagg ttacatggga aaaggtatat
     8161 attatgcagt acaagatgaa gtaaaagttc cagttaaata ttcagcaact actataggta
     8221 tagctgcagc tttaggattt tcaccagatg tattccaatt tgcattaata ggacactgga
     8281 tagatactta tggcaataaa ggttacacat atacatttat attccaaata gcaattctag
     8341 ttattggaat actatcttgt ttatatatat taaaggttaa aaagagaaga gcaagtaaat
     8401 tagaagctta aatattcaat ttggtaatta agtatatata aatacaatac ttattttgat
     8461 gaatgtgatt gtataaataa cataaaaaat atcaatagat taagttttat aataatgttt
     8521 agagaataaa gtataaaaat caaaatgtta tcaagttttc taggttttaa cataaagtta
     8581 cccctgaagg tgtaaaattg agtggcaatt attatctatt ttcttaccta cataaaccta
     8641 tttaatttaa attctaagag attttcaaaa tagatggtag ttgtactcaa ttttaaacct
     8701 cataataaat acgaaaattt aaaatctaac tagatact
//
LOCUS       DAENCV010000148         8735 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAENCV010000148 DAENCV010000000
VERSION     DAENCV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.150"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            157..1605
                     /locus_tag="KQD82_003304"
     CDS             157..1605
                     /locus_tag="KQD82_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG4381756.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1631..4021
                     /locus_tag="KQD82_003305"
     CDS             1631..4021
                     /locus_tag="KQD82_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG4381757.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4023..4694
                     /gene="pgmB"
                     /locus_tag="KQD82_003306"
     CDS             4023..4694
                     /gene="pgmB"
                     /locus_tag="KQD82_003306"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG4381758.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKYDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4733..6136
                     /locus_tag="KQD82_003307"
     CDS             4733..6136
                     /locus_tag="KQD82_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4381759.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTIIGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIGKLRKCVK"
     gene            7050..7709
                     /locus_tag="KQD82_003308"
     CDS             7050..7709
                     /locus_tag="KQD82_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381760.1"
                     /translation="MIIEVSNLSKVVDLLNTELTILRNIDLYIEEGEFVSIMGASGSG
                     KSSLISILSGLDKNYTGNVKVCGENLSKLTDDELAEYRNRKIGIVFQSFNLITSMSVE
                     ENIKLPLIFSDNKDYKIVDKMIKKVGLTSKTKNNIKQLSGGEKQRVAISRALVCSPNI
                     LFADEPTGSLDSTNSHAIMKLFKNINNEYGTTIVMVTHDIQMAEYSDRIISIKDGTII
                     K"
     gene            7721..>8735
                     /locus_tag="KQD82_003309"
     CDS             7721..>8735
                     /locus_tag="KQD82_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381761.1"
                     /translation="MIFHEVYKIYSKNSLKNNKLLKISCIISIVFATAILLFTRVLTD
                     TYSSEVKRNMAITNGGDIKIQNIEYNKYYFTSEQIDKLEELKGKESIDFQLGSSMNTN
                     ILSNGMIESTELTVVNNIKMQNTYLKLRNENDIVLSQKTANKLNVNIGDKVFLKLHSS
                     VYDDTYFKVSDIIPKRFSLSTAQKEAEVGQEVVGESYVYLPNETKYNVAYINIINKEN
                     VKNIKNEIKNVFKNHFEVRTTDELEQSIIKRISMQLDSINLIGAISLLSTGICLCSTF
                     IVFILNRINDFSTFKALGIKKRSLSFLVFTELMSITIKGIIIGIILGIPITIFYINLQ
                     HINL"
BASE COUNT         3549 a          853 c         1499 g         2834 t
ORIGIN      
        1 tgaaagtatt gaaaatagac aatattaatt atagtgagta aaaaatataa ttggattttt
       61 aaattgaatt atttaaaagg tatagtatga aaaaaatctt ataactaaat atgagataga
      121 aattataaaa ctttatattg tgaaagaggt agatgcatga aattagggaa agatttttta
      181 tggggagggg caatttcagc caatcaaagt gaagggtttt atttagatgg aggaaaaggt
      241 ttatctcagt ttgataaact cccattaaat gataagagat taaaagatgt atgtttagat
      301 gaggataata ttttaaatga aaataatcaa tactatccaa gtcatatagg gattaaattt
      361 tgtgaaaatt acaaagaaga tatagcaatg ttagcagaaa taggttttaa atgttttaga
      421 ttttctatat cttggtcaag aatattccca acaggagaag aaagtgaacc aaatgaaaag
      481 ggattacaac tttatgataa tataataaaa gaattaaaaa aatataatat agagccatta
      541 ataacgataa gccactttga tataccttat tctctagttg aaaaatattc tgggtgggct
      601 gatagaagag tggttaattt atatgtaaaa tatgctgaaa ttttgatgaa aagatataaa
      661 gatgatgtca aatactggat tccttttaac gaaatgaata tgataataca tattccatat
      721 ataggtgctg ggttaacttt tatagctgat gaaaatcaac ttcagaaaaa atatcaagca
      781 gcacaccatc aattattagc taatgcaaaa actatagaaa taggaaggta tataaataaa
      841 gaatttaagt ttgggtgtat gttagctgct ggaaaaacat atccatacac atgtaaacca
      901 gaagatgtat ttgcaacttt agagcatgaa aggcataatt tgtttttttc tgatgttcaa
      961 gttaggggtg tgtatccaag ctttatgaat tattatttaa aatcaaatca aataaaatta
     1021 ataataaaag aggaagattt atcaacactt aaggataata ctgtagattt tgtatctttt
     1081 agctattatt ctagtgcttg tagttcagct agagctgaag gtttagaaaa atcaaaggca
     1141 aacggaccag aaaccataaa aaatccatat cttccaaaat caaatagtgt atggcaaaac
     1201 gaccctgtag ggttaagaat aactatgaat cagttatatg aaaggtatca gttaccttta
     1261 tttatagtag aaaatggact gggtactcaa gatattgtaa atgaagatgg aaaaataatt
     1321 gatgattatc gtatagatta catgaaagaa catattaaaa atatgattct tggagtaaat
     1381 gaagatggaa tagagcttat aggatatctt atgtggggat gtatagatgt aaccagtgta
     1441 agtgaaggaa agatgacaaa acgatatggc ataattcatg ttgattctga tgactatgga
     1501 agaggctcat ttaaaagaac taaaaaacaa agtgcttttt ggtataaaaa agtaatagaa
     1561 acagatggag aaattttatt taatgatatg gaaactcatg cataattcat tttaagaaag
     1621 gagtgttatt atgagtttaa tatttactac taaggaccaa cttatagagc aagggtttag
     1681 taaggataca ataaaatcca atgaaacgct gtttacacta gcgaatggac atttaggagt
     1741 tagagggaat attgaagagt ctgattttaa taatgagtat gtagataata tgggtacata
     1801 tgtaaatgga ttttatgaag aatctccaat tacatatgga gaaagcgctt atggctatgc
     1861 aaaacaaaat cagactatct gtaagttacc taatgctcat atagtaaatt tctctattga
     1921 aggagattat tttgatttat caaaaggtat tacaagtgaa cattctagga tactagattt
     1981 aaatgaaggg attcttaaac gttcatttat atgggaaaac tcttgtggta gaaaagttaa
     2041 agtatctata gaaagattag tatcacaaga tatagaagaa ttaatgatga tttcatatac
     2101 aataacacca ttgaatttta atggaaaaat aaaatttaaa aataaaatgg aaaatagtat
     2161 ggagaagttt aattgtgaaa aagtagatga cccaagagtg gcatctataa ataagaagca
     2221 atttaaaatt aatgtaaaaa aatataacaa taagtatttt atgaatttag ttacagaaag
     2281 aagtagatta gggttatggt gtgggtttga ttcatatatt acttgtggaa atattacaaa
     2341 tgagacctat aatgtttcag atgaatgtat tgagcgagaa atagaagtct atgccaaaaa
     2401 agatgaaagg ttaaatttag aaattgttgt aggctatagt agagtatatt ctgatttaaa
     2461 tctaaatgaa gaacgtttaa aatcattaga atgtctggag tcgttaaaat gtttagaatc
     2521 agaagcacat ttaaaatcat tagaattggt tttaagagaa gcatgtaata ttggatttga
     2581 taaagtcaaa aatattcaga gaaagaaaat gaatgagttt tggacaacct ctgatgtaga
     2641 gattgaaggc gataaaactc ttcaactggg gataaaaacc aatttatttc atatttatca
     2701 atcagcagga agagatggaa agactaatat atcagcaaaa ggtcttacag gtgatggcta
     2761 tgaggggcat tatttctggg atacagaaat gtatatatta ccatttttta tatatactca
     2821 accatccatt gctagagctt tattagatta tagatactca atcttagatg catcaagaga
     2881 aagagctaga gaacttggaa taaataaagg ttgcttatat tcatggagaa ctataaatgg
     2941 taatgaatgc agtgcatatt atccagcagg aacagcacag tttcatataa atgcagatat
     3001 agcttatggt attagaacgt attttgaagc aactaatgat gaagaattta tgatgcaaaa
     3061 tggcttagaa attcttatag aaacagcaag attttgggtt gaatttggag attatataga
     3121 atcaaaaaat aataaattct gtataaatgg agtaacagga ccagatgaat atacagcaat
     3181 agtaaacaat aattactaca ctaatttaat ggctaagtat aatttaatat atgcagtaca
     3241 gatggtagat aaatttaagc ataatttatt ttcaaatgaa gtatttagaa aagttaaatt
     3301 acaagaatat gaagtgaaaa actggataaa tgccgctcaa aatatgtatt taccatatga
     3361 tgaagagaag aaaataagca aacaagacga ttcattttta gaaaaatcag tatgggattt
     3421 taaaaataca ccaaaagaaa aatatccatt acttcttcat tatcatccat taacaatata
     3481 tagatatcaa gtaaataaac aagcagatac tgtactttca gcgttgcttt tccctgatga
     3541 gtttagctta gaacaaaaga agagagattt tgaatactat gaaaaaataa ctactcatga
     3601 ttcatctttg tcaaggtcaa tatttggaat gatggcaagt gaaattggaa atcatgaaaa
     3661 agcttataac tattttatgg acacagcatt aatggatatt aataacctac aaaaaaatac
     3721 aagtgatgga atacatgctg ctaatatggg tggaacatgg atgagtttag tatatggatt
     3781 tgcaggaatg aaaataaaaa atgataaatt aagttttgaa ccaaggatac ctaaacactg
     3841 gaaaaatatt aaatttaata tattatttag agataattta gtttctgtaa atatagaaga
     3901 atataaatct tattataagt taatcaaagg tagaaatata accatattac atgataataa
     3961 agaaataaaa ttatcaaaga acataataga agttaataat tgttttaaag ggagtaatta
     4021 atatgaaagc agtcttattt gatcttgatg gagttataac agatactgct aagtatcatt
     4081 ttgaagcttg gcatgagata gcaaaatcgc tagatataga aatagatgaa acttttaatg
     4141 aaaaattaaa aggcataagc agggaagaat cattagataa aatattgtta tctgctaata
     4201 aagtagatag ttatactaaa gaggaaaagt taaaggtgtg tgaagaaaaa aataacattt
     4261 atataaagtt aataaactca ttaacatata aagatatatt accagggata aatgagctta
     4321 taaaagaact taaaaaaaat aatgtgaaat tgggaatagc atctgctagt aagaatgcac
     4381 ctaggatatt agaatctttg ggaataaaag atgaatttga ttgtatagta aatccatctt
     4441 tattaaaaag gggaaaacca tatcctgata tttttattga agggagcaag attctaaatt
     4501 taaaacaata tgattgtgta ggaatagaag attcttattc aggtattaaa gctataaatg
     4561 ctgctaatat gacatcaatt ggtgtgggga aaaaagaagt actaggaaaa tatgaccata
     4621 ttgtagaaga tactagctta ttaaattaca atacactatt acaagcttgg agtaaaaaga
     4681 ttaatttgga ttaaaattat attaaaaatt attttaaaag agaggataag gtatgaagaa
     4741 taaatatgaa gaagtagctc aattgataat tgatggactt ggaggaaagg ataatataat
     4801 tgacttaact cattgtatga cacgattaag gttcatctta aaagatgaaa agaaagtcaa
     4861 tgcagataaa ttaaaatcta tagataaggt agttggagta aactcaactt caactcaata
     4921 tcaggtcata ataggtaatg aggtaggtgt tgtatttaat gcaataataa ataaggggat
     4981 aaataccaca ggagatatta aaagcgatat aaaaaataaa aataaaaaaa atagtatttt
     5041 aaataatata atagatacaa taattggttg tatgacacct atgataccag cccttacagc
     5101 atctgggatg ataaaagtta tattatctct ttgcaccact tttaattggc tatcatcaaa
     5161 ttcaagtaca tatagagtat tagatataat aggagatgta gcattttact ttatgcctat
     5221 aatacttgca ataaatgcag ctaaaaaatt taatgttaat acatcaatag cagttatagt
     5281 agtaggtgtg tttttacatc caaatttctc tgcatgggta tcaagtgggg accctatatc
     5341 atttatagga gtaccaattc aaggtgttat atatgcagca tctgttatcc ctgcactttt
     5401 aacagtatgg atgatgtcat atatagaaaa attcattgat aaattaacac ctagcatgtt
     5461 aaaaacaatt ttaaatccaa ctttagtatt attaataagt gcgccaatag cattaatagt
     5521 aattggacca ataggaaatt tcttaggaga gggattagct agtataataa atttactaca
     5581 aggaaggtta ggatttataa tggtttgttt attagcagct gcaatgccat ttatcgttat
     5641 gacagggatg catcatgcat tgactcctat attcatatca gcatttgcaa ctacaggaca
     5701 agaatcgcta atattaatat ctcaagtttg tgctaattta gcgcaaggtg gcgcgacatt
     5761 agctgtagca ttaagaagca aagatagtaa gatgaaacag ttagcatcag ctgcatggat
     5821 atcagctatt atgggtataa ctgaaccagc tctttatgga gttacattga aacttaaaaa
     5881 acctgctatg ttagcatcta taggagcagg aatcgcaggg ttatttgcag gaattactca
     5941 tgtgacttta tatgttccac aaaatagtat aatggcagta cttggattat caggggaaaa
     6001 ggggatttca aatatagtaa atggacttat agttatattt atagcattgg ttgctccatt
     6061 tattatgaca tatgtttttg gagttgaaaa aaatgttaag gaaggtatag gtaaattaag
     6121 gaagtgtgtt aaatagtatg agataaaaaa attcatgata aatatttata ataaaagtga
     6181 ttaacctata aaacttagta aataatagtt atataataat taagataaac tcaatttaaa
     6241 actttaaaaa atacgattct taaatagtca aactttagta aaatatataa aaatatgttt
     6301 tgttaaggtt tggcatattt tttattttgg agaagaaatt tgatacctat attcctacta
     6361 caggaagatg gtctcttggg tgtactatag gatttataat gtataaaatt gtacatgtaa
     6421 aagttattag tccattagga tggtgattaa taaataatat gattaataga atagtttagc
     6481 cttgtattgt aatgtttatt aaagaatcat tttatgccat aagttataag cattttttat
     6541 gtggagagtt ctataaaatg taataaaaaa gtagttttat aatagtatct gtaattctta
     6601 aatatttatc atgaaatttt ttataaatta tacttataag atgatatcaa gtattcgcaa
     6661 tattctgttg aaatttattt gaaagagtca tatatttata acataaagtg ttataaatat
     6721 ataaattttg gggagagatt ctgtatatct aagctaaaac ttagtattaa attaaatccc
     6781 acttccgaat tattgatgat attatatttt ttagatttat cattgaaaaa gtttattttt
     6841 tataatttta ctaacttttg gtcaagcaat cttatgtata ataagaaata taatgaccta
     6901 ttaagatttg tacagtttta gaaaatatat cattctcaaa aaagcttgct tgtataagtt
     6961 tctctaatat tttgtgtcct atatactctg ttttaattaa taatcaatct taaaagtttt
     7021 gtatgaaagg aaataatggt atgaaaaaaa tgataattga agtttcaaac ttatctaaag
     7081 ttgtagactt gttaaacaca gaacttacta tactaaggaa tatagattta tacatagaag
     7141 aaggagaatt tgtttctata atgggtgcta gtggtagtgg gaaaagctct ctaataagta
     7201 tattaagtgg tcttgataaa aactatactg gaaacgtgaa agtttgtggt gagaatttat
     7261 cgaaattaac tgatgatgaa cttgctgaat atagaaatag aaagataggg attgtatttc
     7321 aatcatttaa cttaattaca tcaatgtctg ttgaagaaaa tataaaattg ccattgattt
     7381 tttctgataa taaagattat aaaatagtag ataagatgat taaaaaggtt gggttgacat
     7441 caaaaacaaa aaataatatt aaacaattat caggtggtga gaagcaaaga gttgctattt
     7501 caagagctct agtttgttct ccaaatatac tctttgctga tgaaccaact ggatcattag
     7561 atagtactaa tagtcatgct ataatgaaat tgtttaagaa tataaacaat gaatatggta
     7621 ctacaatagt aatggttact catgatattc aaatggcaga atatagtgat aggattataa
     7681 gtattaaaga tggaactatt ataaaatagg tggtgataca ttgatatttc atgaagttta
     7741 taaaatatat agtaagaata gtttgaagaa taataaatta ttaaaaatct cttgtataat
     7801 atctattgta tttgccacag caatattact ttttacaaga gtgttaacgg atacatattc
     7861 aagtgaagtt aagagaaata tggctataac aaatggtgga gatataaaaa tacaaaatat
     7921 agaatataat aaatactact ttacaagtga acaaatagat aagttagaag aattaaaagg
     7981 taaagaatct attgattttc agttgggaag tagtatgaat acaaatatat tgtcaaatgg
     8041 tatgattgaa agtactgaat tgactgtagt aaataatata aaaatgcaaa atacatattt
     8101 aaaattaaga aatgaaaatg atatagtttt atcccaaaaa acagctaata aattaaatgt
     8161 aaatatagga gataaagtgt ttttaaaact acattcttca gtttatgatg atacatattt
     8221 taaagttagt gatataatac caaaaaggtt ttcattaagt acagctcaaa aagaagcaga
     8281 ggttggccaa gaggtagttg gagaaagtta tgtatatttg cctaatgaaa ctaaatataa
     8341 tgtagcttat ataaatataa taaacaaaga aaatgtgaaa aatattaaaa atgaaataaa
     8401 aaatgtattt aaaaaccatt ttgaagttag aacaactgat gaactagaac agtcaataat
     8461 aaagagaata tctatgcaat tagattcaat aaatttaatt ggagctattt cattattaag
     8521 cactggaatt tgtctatgtt ctacatttat tgtatttatt ttaaatcgaa taaatgattt
     8581 ttcaactttt aaggctttag gaattaaaaa aagaagttta tcttttctag tatttactga
     8641 acttatgtca attactataa aggggattat aataggtatt attttgggga ttcctataac
     8701 tatattctat attaacttac aacatattaa tctat
//
LOCUS       DAENCV010000149         8718 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAENCV010000149 DAENCV010000000
VERSION     DAENCV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.151"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(89..769)
                     /locus_tag="KQD82_003310"
     CDS             complement(89..769)
                     /locus_tag="KQD82_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381762.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(766..1692)
                     /locus_tag="KQD82_003311"
     CDS             complement(766..1692)
                     /locus_tag="KQD82_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381763.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKLNSGEIVMFGKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(1765..2682)
                     /locus_tag="KQD82_003312"
     CDS             complement(1765..2682)
                     /locus_tag="KQD82_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4381764.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(2682..3371)
                     /locus_tag="KQD82_003313"
     CDS             complement(2682..3371)
                     /locus_tag="KQD82_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381765.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKSGIALNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGMEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(3779..4084)
                     /locus_tag="KQD82_003314"
     CDS             complement(3779..4084)
                     /locus_tag="KQD82_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381766.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(4859..5077)
                     /locus_tag="KQD82_003315"
     CDS             complement(4859..5077)
                     /locus_tag="KQD82_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381767.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(5078..5485)
                     /locus_tag="KQD82_003316"
     CDS             complement(5078..5485)
                     /locus_tag="KQD82_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381768.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIEATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(5571..6059)
                     /locus_tag="KQD82_003317"
     CDS             complement(5571..6059)
                     /locus_tag="KQD82_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG4381769.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(6264..7373)
                     /locus_tag="KQD82_003318"
     CDS             complement(6264..7373)
                     /locus_tag="KQD82_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG4381770.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIAKGKREEKF
                     NIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(7747..7917)
                     /locus_tag="KQD82_003319"
     CDS             complement(7747..7917)
                     /locus_tag="KQD82_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381771.1"
                     /translation="MDSNNNTIKSTVKKGISFDSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(8089..8478)
                     /locus_tag="KQD82_003320"
     CDS             complement(8089..8478)
                     /locus_tag="KQD82_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381772.1"
                     /translation="MLADPCEEMIDKYLNKGTMYALYDNKELTCIAVVNEISKEICEL
                     KNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSESGVPFYEKFGFVYSHKIKN
                     FFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         2982 a         1405 c          840 g         3491 t
ORIGIN      
        1 ctaataatat taataatagc tgatataata gcaaatcatt attatcccaa gaagatacaa
       61 ttaataacta ccttcctagc cacagtatct agtcaatttc agtaacttta tgatttagta
      121 tataatatat accaatcaca gttataccaa ctataataaa aacagaaatc aaactgtctt
      181 gagttgtatt tgtaccagct aaaataaatt gtcttaaatt gataaaaata attgaactga
      241 caagggtaac aggtattgat tttcttatca ttccaacaac taatgtccat atacctaatg
      301 agcaaaatac aataatactt ttaattgctc ctggtatcca ataagataaa attgaaatat
      361 gaaaatctcc aataactaaa ttaaaatact tatccaacac aacaacgaat gtaaaacaac
      421 agatatatcc aactatcatt gatgatatca taagagtcgt aatcaaaagt aactttgata
      481 aaataagttt tttacggtca agtggataag agaataaaat caagattgtt ttatttgtat
      541 attcatttat tatatatgcc acattcaaca cagcaaataa tataatatag taaaaacaga
      601 aaaataaact gattgtccta aatgtaacat cataatcata tattgaagta gatttatcat
      661 tcaatccagt aaagacaaaa aacatacata agaaaatccc cagcattgaa aataaaaaat
      721 atctttttag cccaactttt tttagctcta gtttcattaa atgtatcata gtgtttcttt
      781 acctcctgta cctgtagttt ggaagaagta atcttctaaa gaactttgtt tacgtccaat
      841 gctatttata ccaatttgat tttgaataaa tattttggat atttctttta catcttttcg
      901 taaatcatat atttcaatac aactttccga agtaatttta aagtctttta ttccaaattc
      961 tttttctaac aagtatccag caagttccac attatctaca tctacttcaa cataatctgt
     1021 ttgatatttg tgtatatctg acatagaaat ttctttaata agtttaccat cttgaataat
     1081 tccaatagtg tccgcaattt gttctacctc tgaaagaata tgactagaaa taaatatagt
     1141 tattccataa tctgtattta acttacggaa taattctcgc atttctctta tcccctctgg
     1201 gtcgagtgca tttataggtt catcaagaat caataattct ggtttagtaa gaattgctct
     1261 agctattgca agacgttgtt tcatccctaa agaatatttt gaaactttct tgtttgattg
     1321 ctttgacaat ccgacacatt ccaatacctc tgatatatgt tctttgttat aataacccat
     1381 atattcacag tgtaaatcaa gattctgttt agcagtcaaa ttctcataga aataaggatt
     1441 ttcaataata gtaccaatct tttttaatat ttcaaaatca gtttctgcca ctttcttacc
     1501 gaacataact atttctccag aatttagctt aacaagattt agcaacattt tcattactgt
     1561 acttttacct gccccgtttg gtccaacaaa tccataaatt tctccttttt tcacatgaat
     1621 attaacagca ttaacaacag ctttatcttt atatattttc tttaattgat gggtctctat
     1681 gatatagttc atcatactcc ctcctttcta aaaacagtat acaaaataaa attatctttt
     1741 ctctctttta aatctaactt ttttctaact tttcaatttt actgtaaaaa ctgtttttac
     1801 attaggtcta ctatttacat aaattgaacc tcccatagag tttgcaagcc cttgagaaat
     1861 agccaaacct aaaccattgc ctttttttct gtcagcagta taagctcttg tgaaaatatg
     1921 ctctttttca gtttcagaaa tacctgaacc tttatcctca atatctataa aaacatgttt
     1981 ttccttttca tatagtgaaa tccctaaaaa tctaccatct gaaccatatt ttatagcatt
     2041 atcaattaaa tttttgagta tacgttgtaa tgcttctgtg tcaacatttg catatatagg
     2101 tctatcttca atattaactt caacattaaa gttttcttta tctagcaaat cataaaactc
     2161 taaaataatc tcatgacata attgtgttag attggatttt tgaagattta atatcatatc
     2221 tccagattgg atttttgcca tattaaacag gttatttatt gaattaacaa gctcatttga
     2281 atttgtttgc attttttcca cagttgctct cattgaagaa gataattctt ctttttgact
     2341 ttgaagatat agcatttcaa tataaccctt taatactgtg atgggagttc gtaaatcgtg
     2401 tgatatgttt gtaaatattt gcaatatcgc tttttcactt ctattataat taatttgcct
     2461 actgtaaaac ttttcaagaa gtttatttat atctactgcc gtttctttta caatcgaaat
     2521 atcagatggt actaaaatat aattaatttc atcatcattt gttatgtcac gaagtcttga
     2581 attgatatac ttataatcac tttccatttt ctttgattta tttcgttctt ttaagtagag
     2641 agcaaatagt acaatagaaa ttacaactaa aaataactcc attattcttc ctccattttg
     2701 tagccaattc cccaaagagt tttaatataa ttattttttt ccataccatc agacaattta
     2761 tttcttaacc tgttaatatg agtatttagc acattttcat ttccataata aggttctttc
     2821 caaatagcgt tgtataattg ttcctttgaa aaagcacgtc cttgattagt cgctagtaat
     2881 ttaacaattt caaattctgt atgtgttaag ttcaaggcaa ttccactctt tgaaactaca
     2941 tattttgata tgtttataat caaatcttta tactgaataa ccatatcttg agagtttctt
     3001 gaactgctgt acactgttgc acgtctaata ttagctttaa ttcttgcaag caattctacg
     3061 attgaaaaag gctttgtaat ataatcatct gcaccaagat taagccctag cattttatct
     3121 gtgtcactat ctttcgcagt agtaataata acaggaacaa cactcacact tcgtatatat
     3181 tttaatactt caaaaccact agactttggt aacatcaagt ccagtaaaat aatatcaaat
     3241 gaataatttt taaatatttc aatagctgta tttccatcaa tggcagtttt aacagagtaa
     3301 ttctcactaa tcagaaaatc gtataccatt tctcgaatat tcttatcatc ttcaaccaat
     3361 aagatgtcca ttattattca actccttttc gtcagtatac ttcaaatgat atatttatta
     3421 aaaagggttg tcaataaata actgcttatg atattgtatc tgcaatttgg tgatactctt
     3481 ctaagttaaa gttcttttca ataagcttta ttgaaaaaag aattaaaata tagtatactc
     3541 tcttatcttt atctgaaata tacataattt tcttttatta acatctacaa ttaactattt
     3601 tatattataa aacacctttt aacttttgct aaaaaatatt ttatacataa aaaagaaacc
     3661 acattctagt tttatacaga acatagtttc tttaaaaata atcaaatttg gattttaaat
     3721 aattaaaaac aaacttttat ttgtttttaa ttttccaatt ttatagtaga taatttagtt
     3781 aattagttct taaatgttta tcttctttag taaatttttt tcttattaag aaatatgcaa
     3841 ctattacaac aagttctatt gctaaaaata caccataagt agctatagaa tctatgctat
     3901 ctatgccatt tctaaagaaa attaaagcaa ttcctgctgc tataatacct acagcatttt
     3961 tccctataga attttgacca aaaccataaa aagaagggaa atttcctttt atttcaatga
     4021 atgtcaagat taatccaata gctataaata gtactcctac aacttccata atcatgctag
     4081 gcattttgac ccctccttat taataattaa ctactactat aaaaatattt attattttta
     4141 taattatatt atatatgcga ttacatgttt ttggaagtat atttggaagt aatttcatat
     4201 ttatttaact taattttttt gtgataaaat aattctaatt ttcatataga tattattata
     4261 aattttctag tttatggagt cattcattct ataattcaca aatatgtatt ttttaatcta
     4321 ttaaagtatt cattttctgg cttataagca tggacttctt atctataagc ttgttttttc
     4381 atctaataag ttatttattc tttaatctat gagcttgatt tttcatctat aagtcattta
     4441 ttttttaatc tataagttta gttcttcatc tataaatcat ttatttttta atctataagt
     4501 ttggtttttc atctataaat catttatttt ttaatctata agtttggttt ttcatctata
     4561 aataagtcat ttatttttta atctataagt ttagttcttc atctataaat catttatttt
     4621 ttaatctatg agcttgattt ttacctataa ttaattcatt ctctaatcta ttagcttggt
     4681 ttttatttat aagtcattta ttctctaatc tattagcttt ctttttcatc tataaatcat
     4741 tcattcttta atccattagc ttggtttttc atctataagt catttattct ctaatctatt
     4801 agcttggttt tttacctata attaattcat tctctaatgc ataagtaata catgaaattt
     4861 aaatattatt ttcttcaata aattcaaaca actcttctac agttgtatca aataccttag
     4921 caatatccat tgccagtttt aaagaaggat tgtagcgacc attttctaaa tgtacaatag
     4981 tttcacgtct tactcctact gactttgcaa gttcaccttg tgtcataagg ttcttttctc
     5041 tataggattt aatttttgtc tttagtaata ctgacaccta attgtatatc ccctttctgt
     5101 caaaatatat aaatagaatt aacttcaaca aggattgaat aaacaaaatg actaataaag
     5161 acattcctat atttaaattt gacacattta tgtccaaagt tacaagaaat atagatacta
     5221 tagctacaga aaaaaacaat acttttattg caatatcatt aactcttgat agtatacatt
     5281 ttgatgcctc atcaattaaa tcatcatact ttttattata gacccactga gctcccatag
     5341 ctaataccca aataattata ggaagatatt caatactact tcctttaaaa aaatacttat
     5401 ttactaatgt agcttcaata caataagaaa ttcccaatat ttctgaaata agtgttgatt
     5461 taataccagt ttgttgttta gacatataaa atccccctta aaaagttatg tatttataac
     5521 acctcttgtt ataaatatat aacttttttg aataaatttc aataaaaata ttattttgca
     5581 acaaattctt ttattaattt tacattagat agaggcactt tagatgtcac tccttgcaat
     5641 gtcaaatctt ctatattaac ttcatttaat tttgaacaca gttctaagtc aaatattctt
     5701 tcccaactta ccttcttttc aatttgttca tcttcataaa gctctacagc aatatccatt
     5761 aaaggtaaat gccataaatc aaattctgat aataacacat tgtcttcact tatttctatt
     5821 tcaagtaaaa cacctttttt tcctttttct aaataaccac tacatcttaa atcaggtctc
     5881 ttcgtccaaa cccatatcat actatcatca aattcatata ttctatttgt catttgacta
     5941 tacatccatt tgtatggaat atcaaagtct tctaatataa aagaataatc atttgataga
     6001 tatttattgt ttttattgtt attccaaaat tctaaagtct gtatagtata aaatttcata
     6061 tataaatcaa cccctttgtt tttctataag aaggagatgg ttcaaacttt tattttgaat
     6121 tatctccttt atttatacac ctctttgtac ctattattta tatatctctt tatatctacc
     6181 atatttaaat aacaaagttt tatattaatt taaatttaaa agaggaagct ttgtatttat
     6241 ctagtgccat ctcaatcata gtattagttt tgattataga ctttttcaac ctctgattta
     6301 ttcaaatcat ctataatatc gcatatttca ctcaaattgt atcctttatt ttttaatttt
     6361 atgatgatat taaatttctc ttctcttttc ccttttgcaa tacctttttc gatacctttt
     6421 tcaatacctt cttgaaaaat ttcttcttta aaatttctac ttttattcat aatatacgca
     6481 cctctcttta aatttaataa atagtcctca ttactaccta attcttttaa tctatcaagt
     6541 gctcttttaa taacataatc cgttttagat agtattctaa tatctatatg agaaggatta
     6601 tcaataagtt taccccatat tagaactctg tttgctatac aactggattt acgaattttt
     6661 ggaagttcta taaaatataa tgcagtttta ctagaaaaaa tatctggatg cccgtccatt
     6721 gttaatctca ggcaatttac ataatcttct atgtgattat attttacatg atttagtata
     6781 tgtattccaa cagtttttcc aatttcttta tattctttac cttcttctaa ctttctacca
     6841 tgtacacgac taagataata ttcagctctt tccaaataat cctcttcaag agctctttgc
     6901 atctcaatat ttatatggtt acgttcttca tcttcagcca aaatatcaag tctaggtctt
     6961 ttctcaccaa tgtaattaat tgttgggtcg gtttcaacct taattattct ttttatttga
     7021 gtgaactgtt tgatattcct attagcatat ggagcatctt tgaggatatc atttataaaa
     7081 tttgcaagta aatcgtaatc attatttaaa gtaaacaatg atttaaatac ctcatcatac
     7141 tcaggcttag catacccatc ttcagttaca acttctccaa cattttcact agtcaatatt
     7201 tcttttggaa ttaaatcctt gattttatct aatttttcat tagaaaaatc acctctacta
     7261 agtatgtcat ttaatttttt ggtccattct ctgtcctttt cttcaattat agttcgttta
     7321 tactcttttg atgttttaat aatcttagat ttttcttcta atttttcttt catatcaaca
     7381 cctctttttt attattaccc atttcttcat cccaaagtct gaacttctaa gtaaaatttg
     7441 cattattgaa ataagaacct tataaactta tatatcatta ctaaagttaa gactttaaag
     7501 agaagcactt agattacaat agattttaga tagctttctt tttaaaactc ttgttgataa
     7561 atgaatgaat ataagagtat gtattaaaat agagatactc atctttttca ttgtttaagt
     7621 aatgaaaata tattttattt aatacaaaaa agagtgcctt ataaactaaa aagttcactt
     7681 cgagacactc acttacgttt tgtttcttaa acttcacaat attacatact tatttattaa
     7741 tttaatctat gcatacttaa cccaataata aagtacatat atccaactca ttaagccatg
     7801 aaaaatagcc catggaatag atttccatgc agtataagaa ataatcattg ctaaacaaga
     7861 atcaaaagaa ataccctttt taacagttga ttttatagta ttattatttg aatccataat
     7921 taatcctcca aaattcaaaa taaacaagtt attatatatc ttatttgaat ctaatacaat
     7981 ataacagctt ctaaataaat tatactctta ttatacataa tttataaatt ataattccga
     8041 taattatatg aataaattat gtatattaac ctaaaaatat tatcctatct aaaagttata
     8101 tgatagatac aacatgtcaa cacattgtaa ttctccttca aagattggtt ctggataatt
     8161 atctacaaaa aagtttttaa ttttatgtga ataaacaaat ccaaacttct cataaaaagg
     8221 aactccgctt tcagatgtac caacaaccat tgatgaatat tttttactat aaacatctaa
     8281 aagatgataa atcatctttg aggcataacc catattttga aaatgctcat atgtagcaat
     8341 gttttttaat tcacatattt ctttggaaat ttcatttaca actgctatac aagtaagttc
     8401 tttattatca tacaatgcgt acatagttcc tttattgaga tatttatcaa tcatttcttc
     8461 acatgggtcg gctaacatta aaaggtcaat ttgacttttt tatttatttc tatagttttt
     8521 atttctatca taagttctcc accctcactt tattcaaacc ttaatttagc aaacttctta
     8581 cagtacttat ttagttcatt ttgatatata tttttacttt ttaaaatata gtgataattc
     8641 cctagaatat taatactaat aataaattat aatatcagct ctagcaatta cctagaatat
     8701 caatactaat attcatct
//
LOCUS       DAENCV010000150         8703 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAENCV010000150 DAENCV010000000
VERSION     DAENCV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.152"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..1053)
                     /locus_tag="KQD82_003321"
     CDS             complement(<1..1053)
                     /locus_tag="KQD82_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG4381773.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
     gene            1120..1491
                     /locus_tag="KQD82_003322"
     CDS             1120..1491
                     /locus_tag="KQD82_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG4381774.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1528..2202
                     /locus_tag="KQD82_003323"
     CDS             1528..2202
                     /locus_tag="KQD82_003323"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG4381775.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2451..3290
                     /locus_tag="KQD82_003324"
     CDS             2451..3290
                     /locus_tag="KQD82_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG4381776.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3534..3630
                     /locus_tag="KQD82_003325"
     tRNA            3534..3630
                     /locus_tag="KQD82_003325"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Sec,seq:tca)
     gene            3781..4488
                     /locus_tag="KQD82_003326"
     CDS             3781..4488
                     /locus_tag="KQD82_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG4381777.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4630..5466)
                     /locus_tag="KQD82_003327"
     CDS             complement(4630..5466)
                     /locus_tag="KQD82_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4381778.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENKKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5654..6559
                     /locus_tag="KQD82_003328"
     CDS             5654..6559
                     /locus_tag="KQD82_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG4381779.1"
                     /translation="MFFKDIELNSKKELDSYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6568..7752
                     /locus_tag="KQD82_003329"
     CDS             6568..7752
                     /locus_tag="KQD82_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381780.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7867..>8703
                     /locus_tag="KQD82_003330"
     CDS             7867..>8703
                     /locus_tag="KQD82_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG4381781.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLA"
BASE COUNT         3407 a          915 c         1366 g         3015 t
ORIGIN      
        1 tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat cttttatacc
       61 cttagttaaa tatactttct tatccttagt tatgaatata gcatctacat tatcaatttt
      121 attaattaaa tccatacctt tttcaagacc aagtccaaat gtagaagtgg ataaggcatc
      181 acaattaata gatttatcag agattattgt aacactactt aaattgtttt caaaaggata
      241 ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga taaatctttc
      301 ataaattcca gaagtaacaa cagacttatt tgtagtttct atgtttccta tggattcacc
      361 atttttacta gttgggtctt gaatgcctat tttaaaaggt ttatcatttt taccatcttc
      421 taaagtaaat atatttcctc ctaagtttac taaacctttt tttacatcct ctgattttaa
      481 ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat caattttcat
      541 attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt tcttataatc
      601 aataagaggc agaagttttt gtatctcaga atcacttgga actttagctt tatctgttcc
      661 aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat ctgataattt
      721 ggaatattgt atagcttctt ttacaacgaa aaatgtatca tcagaaactc tgacaaattt
      781 tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag tgctcatttt
      841 gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac ttttggattc
      901 ttttacatta aatacagtaa cttcgtttac tgtaccaagg tagtagctag ttttactata
      961 tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta aaatcaatat
     1021 cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg aaattgtgtt
     1081 attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa agacattctt
     1141 cttattttag gcattttggt tgttattaca gcatgttatg ggataataaa tgttataaat
     1201 tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa aactgtttca
     1261 ataaatgcca aagaagaatt taaaatagag aatagagggg ggtataacat agtaaagatt
     1321 catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg cgtacatact
     1381 ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt gagcataaaa
     1441 ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata aaataataaa
     1501 aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg ggatgaatta
     1561 ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct tatagaagaa
     1621 tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt aaaacacact
     1681 tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg agataatcaa
     1741 gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc tttattaaat
     1801 atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg atatttaatg
     1861 ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag agcgcatgaa
     1921 gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat atctatttta
     1981 agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg taaaaaagta
     2041 gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag cccattgtca
     2101 gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat tttgaaaaag
     2161 ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa ggtaacaaaa
     2221 tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata tgcttaaatg
     2281 gttgaaaaaa ctatgtatgc atatttctat ttttttgtca agttgacaac tcttgaaaaa
     2341 atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac aaagcagaat
     2401 tttatgaagc aaaatatata gtgtaaaaaa tcttagggag gaaattttat atgaatttta
     2461 aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt ggaatattat
     2521 cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa gaaagaacta
     2581 tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt agctatgatg
     2641 caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat aaaatagaag
     2701 taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt gaagttacag
     2761 ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa ggaaagtctg
     2821 gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagta gaaaaggaat
     2881 ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa gggacaaagg
     2941 tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct tcatcatcat
     3001 ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact gcatatactg
     3061 gtcacagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct gtagacccta
     3121 aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg tttactgcag
     3181 aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat gattcaaaaa
     3241 cagtttataa ctggggaaga aaaactatag atatacgtat attaaaatag ttaaataatt
     3301 tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt atagattcta
     3361 aaaatataat ttccagtttt ttattttgga tatgtaataa aacaggtaaa ttttaaccat
     3421 gtatagataa aagctattta aattataatt tactatcata taaatctata aaaattgttg
     3481 caaagagtca aaatatatga tattatagat ttcgttgaga aggatttcat tatgggagtg
     3541 gatgggtgcc ggtgtgccct ctagtcttca aaactagtat gaggggttaa gagcctctta
     3601 ggtgggttcg attcccacgc attcccgcca atatgtaaaa ggtatctttt aattaaaaga
     3661 taccttttta aatttatttt aagataattt atttttaata atatataatg ttttgtatta
     3721 tataatgtaa attttctaat actattatat atttagataa aaatctagga gtgtggaaag
     3781 atggaaaata attttacaac gagaactagt tttctagttg gagatgatgg gataaaaaaa
     3841 cttaataatt caaatataat agtatttgga gttggaggag ttggtagttt tactgttgaa
     3901 gctttagcaa gagctggagt aggcaatatt actattgtag attttgatga tgtagatata
     3961 accaatataa atagacaaat accagcccta cattcaactg taggaagata caaagttgat
     4021 gtcatggaag aaagaatatt agatataaat cctaatatca atataaaaaa aataaggagt
     4081 ctttataata aagatactag tgacgaaata ctaactgaaa gatatgatta tgttgtggat
     4141 gcgatagaca tggtaagttc caaaatacat ctaattgaaa cttgtgaaaa aaaaggattg
     4201 aaaataataa gttctatggg tatgggaaat aaattagacc caactaaaat agtcgttaca
     4261 gatatacaca aaacaagtac ttgtcctcta gctaaagtta tgaggaaaga acttagggat
     4321 agaggtatca aaaagttaaa agtagtatat tctactgaac aaccaataga acttaagaag
     4381 aaagtaatga atggtagaaa agtaacacct ggtagtgtat catttgtccc atctgtagga
     4441 ggtttaataa tcgcatctgt aatagtaaat gaactattag gacaataagt tttaaatatt
     4501 ttaatttata aattttatat acaaatttga aggctagaag catgaataca aagcaatttt
     4561 atattcatac ttttagccta attattttat attaccttat tttatgtagt tgtagtttta
     4621 ttttttagat tatgtatcta tttcataatt atattttgta aatataattt ctccatcact
     4681 ataaaaaaca atatctccta ttctatctgt tctataaatt gatacatggt tttgttttag
     4741 attattaata acctccctat gtggatgacc atattgattt ttatatccac atgatattgc
     4801 agctatatct ggactaattt tttttataaa ttctaaagat gaagaagaat tacttccatg
     4861 atgacctatt tttataaaat taatattatt taatttaaaa gaatgtaaaa tttctttttt
     4921 attttcttct tcagcatcac ccataaataa aaaagaatta tcattaaaag ttaatttaaa
     4981 aactatagaa tttaaattag attcttcttg tatatatgat ggacttaata catatatatt
     5041 tatattgtta tctatgttta aaacatcatt tttatagaga tgctgaatag ataagttttt
     5101 atccgtacaa gaatttatta gattttgata agcctcacta tcagttgatt gttcaggcat
     5161 atatatggag tttacattaa atttttttat aatattatca agacttccta tgtggtctga
     5221 atctggatga gttgctatta taatatcaat agtttttatt cttttttgtc taagataact
     5281 aataattata ttttcgctat cttcatcacc tccatcaatt aatatattct tatttgtggg
     5341 tgtttgaact aaaatactat caccttgtcc aacatcaatc atatgtatag atagtagaga
     5401 ttttttgtca cagccgatta aaagtgatat tataataagt attgataaaa aaattttatt
     5461 tctcaataaa aaatcactcc attcattaca tgtttatata aatttatgat gggtaatata
     5521 agatatatat gacttattat attttatatt ttaaacaata taaatgaaat taataccata
     5581 ataaaattgg agttttttat atatacaaaa taaaattttt aaaattaagc aataaaaaag
     5641 gtaggaggat tttatgtttt tcaaagatat tgaactaaat tcaaaaaaag agttagactc
     5701 atattttgat ttagtagatt atgaagcttg tgagtactgt tttagtactt tatatatgtg
     5761 gcaacatgtt tataagacag gatattatat aggtgaagat tttgccgttt tagttgggga
     5821 atacgaagga gatagcttct caatattacc acttgctaaa aaagataaac ttccagaagt
     5881 tgtagatttt gtccttgaat atttttctaa aaataataaa aaaatatact taagaggtat
     5941 tactactgaa gtagtagagt ttttaaaaga aaaatatcca ggtagatttg agtatataga
     6001 agaaagagat ttatttgact atatttatga tgcagaaagt ttaagaactc tagctggtaa
     6061 aaagaatcaa aagaagagaa accatataaa ctatttctta aaagagtatg caggtagata
     6121 tgaagctaaa ttattagata aagaaaactt tgatgagtgt ttagtactaa tgaaggaatg
     6181 ggaaagtaat aaggaagaaa ataatgaatt tgatgaaagt atggatgatg aattaatagg
     6241 tatcaaaaag atatttaacc attatgacat attaaaagat aaggtaaaag tgtttggtgt
     6301 ttatgtagat ggaaaattag aagcttttag tataggagag cttttaaatc caaatatggc
     6361 attgattcat atagaaaaag ctaatcccga tataagaggt ctgtatccat ttataaatca
     6421 acaatttttg gtaagtgaat ttaaggacgt tgagtttgta aatagagaag aagatttagg
     6481 tattgaagga ttaagaaaag ctaaactttc ttatcatcct tgcagatttg ttgaaaagta
     6541 cagtgttagg gaggcttaat agactatatg gagattaggt atgctaaaga agaagagata
     6601 gaaagtataa aagatatttg gagttattgt tttaacgata cggagagttt tatgaagtac
     6661 tattttaatg ataaatataa atctgaaaat actgtagtag ctttagatga aggaaaaatt
     6721 atttcttctc ttcagctaaa tcagtataag ttattactaa atagcaaggt atataataca
     6781 tcatatgtgg taggagtttc tacacttcca gagggaagag gagctggata tatgaacaaa
     6841 gtaatgaaat ttactttaaa cgaattgtat aaaaaagggc aattagtttc tatattgatg
     6901 cctattgact atagacttta tagaagattt ggatatgagc attgttacga ccagattgaa
     6961 tatactatta acacagatga tttaaaaaat tttaagtcaa gtggaaaaat gataaagtct
     7021 aatttatcac aaattgatga tttgatacaa atagatagaa cttttttaaa tgaggttaat
     7081 ggtaatgtgt taaaagatga acattattat gaaaacttat ttaaagagat tcaaagtgaa
     7141 gatggatttt tatacattca tgaaggaaat gaaaaagatg gatatatagt ttattttctt
     7201 caggaagata aaatgtttgt cagagagtta ttttataaaa atatagatgc ccttaaatca
     7261 atgcttaaat ttatatataa tcacaatact caatgtaaga tagttactat atctactcct
     7321 acaatagata agataaggtt tatattagac aaccctaaag atagtgatat aaaaataaaa
     7381 ccttttatga tgggaagagt tataaatgta aagaagttta ttgaagatat agatattgag
     7441 aatgatatta atagctcatt taatttatta atagaagaca agtttataga tgaaaataat
     7501 ggtctattta aaatttctat acagaataag aaggtatctg tagagcagtt agataaaaaa
     7561 ggtgcagaaa agcctcaaga agattttgat attaaattag atataaatac cttaacacag
     7621 ttatcatttt cttatataga tgtaaatgaa gctattttct taaatgatat aaaagatgtt
     7681 tcagaagaaa cactagaaac acttaattgt attttttcta aaaaaaataa ctatataaat
     7741 gaatatatat aggtagagta cgttaaaaga ttttaaaaat cttttgacgt atttttttgt
     7801 ttagataaca atttatcttg tttaaaaatt ttctatttga aaataatcta aataatatac
     7861 ttttatatgg gagtgattat aattcaaata agaagaccat tattaattat attaatgttt
     7921 gtggtagcta tatcatttat cattacaaat aacacaaaaa acgacgaaga acttaatgat
     7981 aagataataa ctatacaagg agttgtcaaa ggaaaaattg aaaagaaaag atacaatcag
     8041 tacaaggtag gggtttttct aataaatgat tatacaaaaa ataaaaattt gaagattggt
     8101 cagaaagtta atattacagg taaattcaaa agtttagata aaatgaagta tgatgatttt
     8161 gattatggaa ggtatataaa aagtactgga tataaaggta tagtgtatat aaataactat
     8221 aaaattattg ataaaaataa actttactct ttaatagggg aaataaagtt ttatattagt
     8281 aaaacttata ggtacttata taagaaaaac tcagatttta taaattctat actattagca
     8341 gaagttgaaa atttgactga ggaacaaaaa gaaattttta ccagaactgg aactagtcat
     8401 gtaatatcta tatcaggtct tcatacagga atattatgtg taattatatc ctttctatta
     8461 agaggtataa ataaattgta taaattatta atactgtcga tttttataac tttatattgt
     8521 ataatggtag gtgcgtctcc atccataatt aggtctatag catttgttat gattttttat
     8581 ttatcaatat ttattgatag aaagaaagat ggaatatcgg cattgtctct aattgggata
     8641 attttaatta tgaataaccc ctatgttata tataatgtaa gttttcaatt gtctttttta
     8701 gct
//
LOCUS       DAENCV010000151         8686 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAENCV010000151 DAENCV010000000
VERSION     DAENCV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.153"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            16..2193
                     /locus_tag="KQD82_003331"
     CDS             16..2193
                     /locus_tag="KQD82_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4381782.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="KQD82_003332"
     CDS             2207..2629
                     /locus_tag="KQD82_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381783.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3037..3840
                     /locus_tag="KQD82_003333"
     CDS             3037..3840
                     /locus_tag="KQD82_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG4381784.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADAVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3833..4501
                     /locus_tag="KQD82_003334"
     CDS             3833..4501
                     /locus_tag="KQD82_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG4381785.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4737..5399
                     /locus_tag="KQD82_003335"
     CDS             4737..5399
                     /locus_tag="KQD82_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG4381786.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5459..6256
                     /locus_tag="KQD82_003336"
     CDS             5459..6256
                     /locus_tag="KQD82_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4381787.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6374..7564
                     /locus_tag="KQD82_003337"
     CDS             6374..7564
                     /locus_tag="KQD82_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4381788.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7673..8422
                     /locus_tag="KQD82_003338"
     CDS             7673..8422
                     /locus_tag="KQD82_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG4381789.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3450 a          869 c         1599 g         2768 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaagaatt taatagatag agaatacata
      841 gaaaagtatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt cgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaact atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1441 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg
     1621 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1801 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2101 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2401 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agaatgactt atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata
     2701 atgaaaactc aatatagctt ttttatggat atagttggat actataatgt gattcttagt
     2761 aatagttttt tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt
     2821 cgaagtataa tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa
     2881 ggtataaaat cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc
     2941 agagctatta aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa
     3001 aaaattaaaa taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt
     3061 atgtttgcct tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat
     3121 aaagaagaac aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt
     3181 aaagataaag ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga
     3241 actaattgta aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa
     3301 gctgataaag cagatgctgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa
     3361 tatgcttttt cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat
     3421 actagtataa aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca
     3481 gtagcaaatg aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat
     3541 tcaagtgcga ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta
     3601 gttgcgcagg atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg
     3661 atagaacctc caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt
     3721 gaaaaatgga caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg
     3781 actaaaatat ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa
     3841 ttttaatttt gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac
     3901 aatactaata tctattgttg catttttttt atcaatatta ttaggtgtaa tacttgcctt
     3961 tttcacacag acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt
     4021 tagaggaaca ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt
     4081 ttcttttatg aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc
     4141 atctgcatat atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca
     4201 ggaagcatgt tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc
     4261 tcaagcagct acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg
     4321 ttcttcttta ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc
     4381 tgcttcaagt tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt
     4441 atctataata ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta
     4501 gatttcaata tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta
     4561 attttagaaa aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac
     4621 tgtataatca agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa
     4681 tttaatatgc gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg
     4741 cttttaatat gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa
     4801 ctgtgatttt ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt
     4861 tatcatctta ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt
     4921 ttagaggtac acctcttatg ccacagttat tctttttata ttttggacta gcatatatga
     4981 gtgattttgt taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata
     5041 tgggagctta tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga
     5101 tagaagcagc atatgcaatg ggattaacaa atttacaaac aatgaaaagg atagtgatac
     5161 cacaggccgt aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag
     5221 ggtcttctgt agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag
     5281 gagcgttgag ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta
     5341 taataactcc tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag
     5401 ttattaagaa aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat
     5461 gataaaaaga aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac
     5521 tggttgttca aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa
     5581 ttcagctgac ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat
     5641 gtggaaagag attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag
     5701 ttctatattt ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat
     5761 gtctaaagaa agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg
     5821 tatagctgta aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt
     5881 tgtagcagtt tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa
     5941 tttgtttcaa gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg
     6001 aagagttagt gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa
     6061 tatgaaagta agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa
     6121 gaaaaacgat gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat
     6181 gcttgcagat ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc
     6241 tgatatacaa aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag
     6301 tatactaaat ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa
     6361 atcaaaatga gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact
     6421 aaaaaataga gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc
     6481 atttaaagaa tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga
     6541 atacataaat attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg
     6601 aaaaactata ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt
     6661 agaatttaaa tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg
     6721 gttgttagga acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa
     6781 attactattt caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt
     6841 acttgaaggt gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa
     6901 aattggaatc gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat
     6961 tggcaagggt gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa
     7021 tgcagtatat tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt
     7081 agtagtgtct gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg
     7141 tacacttcaa gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat
     7201 tctagaatat atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa
     7261 gagtacagga ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag
     7321 tgcttgcaaa atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg
     7381 tgaggatttt gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg
     7441 agataaagaa aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa
     7501 atgtcttagt atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact
     7561 atagagtaca ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa
     7621 ttttgatgta gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa
     7681 agtagaaaat ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga
     7741 gataagtcaa agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt
     7801 acttagatgt ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt
     7861 aaaagtagat gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc
     7921 tatggtgttt caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga
     7981 ggcattgata gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt
     8041 aatagataaa gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg
     8101 acagcaacaa aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt
     8161 tgatgagcct acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa
     8221 atctcttgca aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa
     8281 agaagttgct gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc
     8341 tgatgaaata tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag
     8401 gaaagaagaa gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac
     8461 ccccattaaa tttataagtt agataataaa tttaatggga gtatattttt attattagat
     8521 ttattattta atttaaaatt agaaatgtaa ctatttgaaa attcttttta aaagtatatc
     8581 aaaatatcaa atacataaaa aaataatatt tgacaaagta tcatatgtgt ttacaaaatc
     8641 aactttatgg agttatatag tataggaatt acaatgaata tactaa
//
LOCUS       DAENCV010000152         8624 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.155, whole genome shotgun sequence.
ACCESSION   DAENCV010000152 DAENCV010000000
VERSION     DAENCV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.155"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            342..1436
                     /locus_tag="KQD82_003339"
     CDS             342..1436
                     /locus_tag="KQD82_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381790.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            1662..2078
                     /locus_tag="KQD82_003340"
     CDS             1662..2078
                     /locus_tag="KQD82_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381791.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2260..2829)
                     /locus_tag="KQD82_003341"
     CDS             complement(2260..2829)
                     /locus_tag="KQD82_003341"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG4381792.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            2983..4140
                     /locus_tag="KQD82_003342"
     CDS             2983..4140
                     /locus_tag="KQD82_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG4381793.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKIVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            4305..4660
                     /locus_tag="KQD82_003343"
                     /pseudo
     CDS             4305..4660
                     /locus_tag="KQD82_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5042..5794)
                     /locus_tag="KQD82_003344"
     CDS             complement(5042..5794)
                     /locus_tag="KQD82_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG4381794.1"
                     /translation="MEKKSPIIKNIVDSFRIIDVINEKGSIGIAKISEILSIPKTSVF
                     RIVKTLEEIHVIKQLENSDYTLDYRLLSYAKGTSQENKLISVAEPYLQKISKETGESV
                     NLGIRYQNEVIIVKSIEGEFYQLQTTLIPVSPLYCSGMGKLFLSQESEEFLQDYFNNL
                     EKRTVNTITEYNEFLLAKKRILELNLSIDNEEYEYGLSCYAVPIFDCNNDLICAISIT
                     GPSNRLMHKGEEYLIKVLKEQANNLQTEFSKL"
     gene            6044..6961
                     /locus_tag="KQD82_003345"
     CDS             6044..6961
                     /locus_tag="KQD82_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBG4381795.1"
                     /translation="MKVSIIGAGAVGATTAFTLAKTSFVDEIAIVDIDQNRAKGNALD
                     ILHGLSLMHETRIVSGDYDCAINSDVIVITVGVPEKVGESRLVPLQKNVKILQDIIPK
                     ITSTSPNGLLLVVSNPVDIISYFSQKISGWEAKRVIGLGTTLDSARLNYLIARDLKIS
                     QTDIQGLVIGEHGDSQVVAWSQTSVKGTPFLDYVKSNNISLQEDYCNNLAQEVKDTAF
                     DVWDMKGPNCYCVALAIERVIKAIARNEHAILPVSQPADSEMYISQPHIIGRDGVQRR
                     VILSYDTQELNAFDTSYQSLQDIVKQIEI"
     gene            6980..7660
                     /locus_tag="KQD82_003346"
     CDS             6980..7660
                     /locus_tag="KQD82_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HBG4381796.1"
                     /translation="MNISQTKGFFKFLILTLLGIVFLLIPFRFENGMDTILFHYLKLF
                     ISENHAVIQTIVVLLVCLSATLSLIDQLLPNTILRKQRLLKKLFSTTPFYVFNRIMGV
                     IIAMLCYLQIGPKFIISIDTGGAMLDLGTQLSVLVPLMLLFQTLILEFGAMEFLGQLI
                     GFIAKPLFKLSEICAVNIISAWVGPGNAAIIGTKELFEKGYFTVKESAVIGSQFSISS
                     IGWVVLVC"
     gene            7673..8311
                     /locus_tag="KQD82_003347"
     CDS             7673..8311
                     /locus_tag="KQD82_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HBG4381797.1"
                     /translation="MDHFALIFLGITLIGIILAFINVRIPPISNYPDTYVDGSTTSKI
                     VSSTQGTRLQRAIIQSSMRANQVTLKNFTSKIDNMSFYVLWLTPIIIFWGALALIVSL
                     YTPLLTWVSYPIELVLKLANISEPAITASAIMSGFADNYLPVILGKGLESIESKTIIT
                     MMSILQLIFMSEIATLLQSCKVVKSFKDIIIIFLQRTIIALPFVIIFTKLIL"
BASE COUNT         3085 a         1043 c         1412 g         3084 t
ORIGIN      
        1 ctttttttta tatgatatca atatatcaaa aatgtaataa aatattcaaa tttttacaaa
       61 aatagagaaa atattcaaat atatattgac tattcagaaa aatatgacta atatataatt
      121 tatagagaaa ctaattttat atataatgac tacttagata aaagaatcta aaaaaattat
      181 gagaggagga gtagggtata aaatcagatt ataagtttta agttattata ctaaaaagct
      241 ataaaacagt tatcatgtat tttatgttaa aggaaattgt aggtagttta tcgaattgtt
      301 taatcagaag aaaaaatttc acatggggag gtaactgaga tatgtcagaa aaagtaaatg
      361 ttaaaacagt catcagtttc gcaggtgcgt atgtagcaac agtaatcggt tctggatttg
      421 caacaggaca ggaaataatg caattcttct ctttttatgg ttttgcaggt attattggtg
      481 ggataatttc aatggtatta ttctcatgga tgggtgcttc agttatgtca aaaggtaaag
      541 agcttcaact taaagaacct atcaaaattt atcgtgttta ttgtggtaag tatttaggaa
      601 tattctttga atggtttgga cctttattct tatttggtgt tttcgtagta atgatttcag
      661 gagcaggagc aactttaact gaatactatg gattaaatcc atttgtaggt agagtaggta
      721 tggcagtagt agcattatta actgtttcac taggtcttga taaattatca aagatactag
      781 gtggaatagg gcctataata ataatcttta cacttttagt tggtggtata agtttagcta
      841 aaaacatagg taatatagga gaagctgcac aagttcttag cactgtaaag atagctaaac
      901 cagttccaaa tgcctatcta tctggtgtaa tatatactac ttataacaca attgttgtta
      961 tggcattctt aactggatta ggagcaagtg ctgctaacaa aaaagaagct atttgtggtg
     1021 gtatattagg tggagtagca cttatggctg cagctataat gatgcactta gctattcttt
     1081 ctgatatagg aaacttatat tcaaaagcaa taccatcatt attcttagca gataagattt
     1141 caccagtaat tggagtttta ttctcaataa ttcttatatt aggtatatat acaacagctg
     1201 tacctttatt atggtcagta actaatagat ttgtagatga taaacatcct aagtttaaat
     1261 taataacttt aatagcagct atattaggtc ttataggtgg cttcctacca tttgataagc
     1321 tggttggtat tttatatcca tatactggat atatgggcgt cataatactg gtttgtgttc
     1381 tttatagaca gattacaaaa acatctggat atcaagaagg aatcagtgac aaataagtat
     1441 tttttaatat tgtaaactca agagctatat gaaaacaatg aaaatgtttt catatagctc
     1501 tttttatttt ttagctcgtt tattatgaaa tagaaaaaac atgtttttta gttacattac
     1561 atatatttat aattatgtat aaatatatac taatcaataa tttagagtct aagataagtt
     1621 tataaatcat atatttagaa tatatgggag gagtgataag attgaatgta aagtttaata
     1681 ttaaaggtat aatttatgga gttatagctc ttgtattcgt tataggggga tttatattca
     1741 taccaagtat gtttgtagag aaatctaaac ccatagatta tacagtatta caaagaaatt
     1801 caataccaga aaagatatta gatgtgatgg ataagtatac aaatgaagaa agagcattag
     1861 cggctaaaat cgataacaaa atatacatta tagtgactag aggaaataat aaatatggta
     1921 tagaaatgga caaaatagaa agtgtggttg aggaaggaaa ggatgttttg agagtcaaga
     1981 taaagtataa agataaggaa gattcttttc catacatagt tgttgaaaca aatatgagtg
     2041 aacttccaga tagaatagaa ttaaatgcga cgaaataaga aggtatttta taattaaata
     2101 actgcatatt gtacaataaa gtcatggttt ttattaatgt aagtaaattg tgtatggttc
     2161 ttattgttgt aaaagccatg acttaaaatg cttataaagt aagatattaa cagtagtatt
     2221 tattactaca ttaaatgggg tctggaaata cttaatattc taactatttg gagtgataat
     2281 tctagtttca gtaactatgt agtctaattg tgcatcatga gtttcttttg gaatagaatc
     2341 gaatatctgg aagtcaaatg ctatgccgat agttatagca tcttttctta agttttctaa
     2401 aaatctatca taaaatcctc caccatatcc taatctatag caatgtatat catatgctac
     2461 agctggtaca attacaacat ctaaggcctt aatatcaatt gtaggtgatt tttcgttagg
     2521 ttctcttatt ccatatgctc caattctcaa tccctctttt aaatctgtta tttgagtagg
     2581 gattaatgta tgattttctt ttattgtgat gggacttgat actattttct ttagagacaa
     2641 aagttttttg attaaattat ctgtttgaac ttcattgtta aaatttagat aaagcattat
     2701 attagttgca tttcttatat cttccatttg aagtaatttt tcagttatta aatttgaatt
     2761 actaagtaca aagtttgcac tcttattttt tcttgattca ataacttttt ttctaaattc
     2821 ttttttcata aaataattcc tcccttaata tgttataatg tgtacagcaa ttgtatttaa
     2881 tataaatttt agtataattt attgtaataa agcaatatat ttgggtaaat gtaataaaaa
     2941 taacagctag tcatataata ataatagttt atgtaaaagg agatgtatct tgtgatttca
     3001 aagaagaaag ctattttttt aggggtgatt ttagtaatta taacagctat ggttacatca
     3061 gcatttcagt tgacattggg gaataaagtt gtaatatcga aagaactcta tgaagactat
     3121 aaaaaatatg ataagttgtt aggactagaa tctatcataa aacaagattt ttataaaaaa
     3181 gtatcagata ctgatttggt aaatggagca tcaaaaggtc tatttctagg aacaaatgat
     3241 aagtattctg gttattatac taaagatgaa atggagaatc taattaatga tagtgaaggt
     3301 tcttatgttg gtgtaggtat gtatataggg gcatcaaaag atggtggctt agtagtagtt
     3361 cccatgaaag attctccagc agagaaagca ggtgtaaaat caggggataa attagtaaaa
     3421 gtcaatggaa aatctgtctc atataaaaat tcagatgaag ctgttcgtat gatgaagggt
     3481 aaaaaaggga aaatagttga actgactata ttaagagaag ataaacaatt gaactttaaa
     3541 gtaaaaacag accagataat tgagaagagt atagaaagta aagtaattga taatgattta
     3601 ggatatatag aaattaccca atttatatca agtacatata ctgactttga taaagcttta
     3661 aaagaattaa aagcaaaaaa tatcaaggga ttagtaatag accttagaaa taatcctggt
     3721 ggaatgctag atatatgtaa agaagttgct gatgagttaa ttggtgaagg aactatagta
     3781 tatacaaaag ataataaggg aaatactgag tacttgaaat ctgataaaga aaaattggga
     3841 cttccaatag tagtgttaac taatggagaa agtgcttctg ctgctgaaat attaacagct
     3901 gcaatcgttg ataataaaga aggtatttct gtagggacta caacttttgg taaagggttg
     3961 gtacaatcag ttgtaagatt gaaggatgga acaggataca agctaactac tgctcaatat
     4021 tttacaccta atggggatta tatcaatgaa aaaggtataa agcctactat tgaagagaaa
     4081 gatgaaaata aacagttaga tgtagcaact aagtggctta gagaacagat tgataaataa
     4141 aatttaattt ttataaattt atattgtaaa ataaagaacc ttaataaaag gagtgtctca
     4201 aaattaactt tttagggtct ttaaaataaa gtgtgtattc ttctaaaaag tagtgataat
     4261 tgaaatatta aatatcacta tataaaggaa ggtgcacact ttttatgctt aataaaaaca
     4321 aaatattaaa aaacttattg aagaatatga tgttaaaaca actacagata ttcaagatat
     4381 gcgaaaagat ttatttgcaa gtacaattca tcaaatgtta gaagtatagc ttgatgacca
     4441 tttaggttat gatagatatg acaataagaa taaaaatact aaaaactcta gaaagggata
     4501 tagaaataaa aatattaaat cagattttgg agaaataact ttaaatattc caagagaccg
     4561 aaatggagat tttgaattaa aggtaattag aaattatgaa aatgatatat ctcattctct
     4621 aatagataaa attactgata gagttatacc tttagctcat ttaagtattt attttgaaga
     4681 aaaaatttaa aatctaagtt aataaagaaa tatttcaaaa aaattaagaa attttcttaa
     4741 aggagtaata atttttgcaa aaataaaaaa acatcaaagt aaggctatga tttttaaagg
     4801 catagcctta ctttgatgtt taattattac ttaaagaata aatttttatt tgtagtgaaa
     4861 actccgtaga ttctattaac atgtatacag caggtttttg ctttaattta tttagagtca
     4921 cgcaatatct atatcatggg taccaccata gttgtatagg aagttatttc accgaatcct
     4981 tttcttactt tatcgtatat tatattgttg tattattctt aacattagtc taaagtactt
     5041 tctataattt actaaattca gtttgcaagt tattggcttg ttctttaagg actttaatta
     5101 aatattcttc tcctttgtgc attaatcgat tgctaggacc agtaatacta atagcacata
     5161 ttaaatcatt attgcaatca aaaataggaa ctgcataaca agacaatcca tattcatact
     5221 cttcattgtc tattgataaa tttagttcca gtattctttt tttagctaac agaaactcgt
     5281 tatactctgt gattgtattt acagtacgct tttctaagtt attaaagtaa tcttgaagaa
     5341 attcttcgga ttcttgactt aggaataatt tccccattcc tgaacaatac aaaggactaa
     5401 caggtataag ggttgtttgt aattgataaa actctccttc tattgatttt acaataataa
     5461 cttcattttg ataacggata cctagattta cggattctcc agtttctttg ctgatttttt
     5521 gcaaataggg ttctgctaca gaaattaatt tattttcttg gcttgttccc ttcgcataag
     5581 ataataaacg ataatcaaga gtatagtcgc tgttttctaa ttgctttata acatgaatct
     5641 cttcaagtgt tttaactata cgaaagacac tagtcttagg aatagataat atttctgaaa
     5701 ttttagcaat acctatactt cctttttcat taataacatc aattattcta aaactatcaa
     5761 ctatgttctt gataatagga ctcttcttct ccataattgc ccaccacctt tcttttaaat
     5821 tctaagtcta attagttaaa atggcaagac gtattttcaa acttcatata tgtaaagctc
     5881 actaattatt agtatttttt cactaatttt agttgtttct ttataaaaaa gagataaaaa
     5941 caaaaaaact ttgttattta ttattaaagg tattgactat atgattttat aatagtatta
     6001 taattttgga acgtgttccg aaattataaa agggggtaat aaaatgaaag ttagtattat
     6061 tggtgcaggt gctgtaggtg ctacaactgc ttttacactt gctaaaacaa gttttgtaga
     6121 tgaaattgca attgttgata ttgatcaaaa ccgtgcaaaa ggaaatgcat tagatattct
     6181 tcatggatta tctttaatgc atgaaacaag aattgtttca ggtgattatg attgtgcaat
     6241 taactctgat gttattgtaa ttactgtagg agtacctgaa aaagttggcg aatcacgttt
     6301 ggttccatta caaaaaaatg taaagatact tcaggatatt ataccaaaga tcacaagtac
     6361 gagtcctaac ggattattat tggttgtaag taacccagtc gacatcataa gttacttttc
     6421 tcaaaaaatt tcagggtggg aagcaaaacg tgttattggt ttaggtacaa cccttgattc
     6481 cgctcgttta aattatttaa tcgctcgtga tttaaaaatt tcacaaactg atattcaagg
     6541 tttagtaatt ggtgaacatg gtgactctca agttgttgct tggagtcaaa caagtgttaa
     6601 aggaacacct ttcttggatt atgttaaatc taataacatc tcgttacaag aagattactg
     6661 taataatttg gctcaagaag ttaaagatac tgcgtttgat gtatgggata tgaaaggacc
     6721 aaattgttac tgtgttgcct tggcaattga gcgtgtaatt aaagcaatcg ctcgtaatga
     6781 gcatgcaatt ttaccagtta gtcagccagc tgattcagaa atgtatatca gccagcctca
     6841 tataattgga cgtgatggtg tacaaagaag agttatctta tcttatgata ctcaagagtt
     6901 aaatgcattt gatacgtctt atcaatcatt gcaagatatt gttaaacaaa ttgaaattta
     6961 attaagggag aaggatttca tgaatattag tcaaacaaag ggtttcttta agttccttat
     7021 cttaacttta ctaggtattg tatttttatt gattccattt cggttcgaaa atggcatgga
     7081 tacaatatta tttcactatc taaaattatt tatttcagaa aatcatgctg ttattcaaac
     7141 aattgtagtc ttgttggttt gtcttagtgc aaccttatct cttatagatc aattgttacc
     7201 aaatacgatt ttacgtaaac aaagattact taagaaactg ttctcaacga cacctttcta
     7261 tgtatttaat agaatcatgg gtgtaattat tgctatgctt tgttatttac aaataggtcc
     7321 taaattcatc atttcaatcg atactggtgg cgcaatgctt gatttaggaa cacaactttc
     7381 tgtacttgta ccattgatgc tactatttca aacacttatt ttagagtttg gtgctatgga
     7441 gtttttagga caattgatag ggtttattgc aaaaccatta tttaaacttt cagaaatttg
     7501 tgctgtgaat ataatcagtg cctgggttgg tcctggtaat gctgcaatta ttggtactaa
     7561 agaacttttt gaaaagggat actttacagt aaaggaatcc gctgttatcg gaagtcagtt
     7621 ttccatctca agtattggtt gggttgtact ggtttgttaa gttttagaca caatggatca
     7681 ttttgcttta atattcttag gaataactct gataggtata atcttagctt tcataaatgt
     7741 aagaatccca cctatttcta attatcctga tacttatgtt gatggttcaa caacttctaa
     7801 aattgtctct agcacacaag ggacacgact gcaacgtgct attatacaat caagtatgcg
     7861 tgcaaaccaa gttactttaa aaaattttac aagtaagatt gataatatga gcttctatgt
     7921 tttatggcta acacctatta ttattttctg gggggcatta gcacttattg tttcattgta
     7981 tactcctcta ttaacttggg tatcttatcc aatagaatta gtattgaagt tagcaaatat
     8041 ctctgaacca gcaattactg ctagcgcaat tatgtcagga tttgcggata actatttacc
     8101 agttatcttg ggcaaaggat tagaatccat tgaaagcaaa acaatcatta ctatgatgag
     8161 catcctacag cttatcttta tgtcagaaat cgcaacatta cttcagtcat gtaaagttgt
     8221 aaaaagtttt aaagatatta ttattatatt tttacaaaga actattatcg ccctaccatt
     8281 tgtaattata tttacaaagc ttattctata agatttaaag gttttaaagt gcttgaatga
     8341 ttattcttta atagtctttc ttttcataat ttagattgat gaacattcct tgaaatatct
     8401 tttgtataag ggagtttgct aaaatatatt gttaattttt taatatttat ttaatttctt
     8461 atttaaagaa attttgaagt tatcacttca aatagttata ttgaatattc tatttatttt
     8521 tgtgattttc aaactatcat tatgtaaagc attatatagc caacctggat atctaattga
     8581 atttgttaaa aattaaattt ataaagataa aatatgtgaa ttat
//
LOCUS       DAENCV010000153         8435 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.156, whole genome shotgun sequence.
ACCESSION   DAENCV010000153 DAENCV010000000
VERSION     DAENCV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.156"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            225..1211
                     /locus_tag="KQD82_003348"
     CDS             225..1211
                     /locus_tag="KQD82_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG4381798.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1380..1528
                     /locus_tag="KQD82_003349"
                     /pseudo
     CDS             1380..1528
                     /locus_tag="KQD82_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1547..3028
                     /locus_tag="KQD82_003350"
     CDS             1547..3028
                     /locus_tag="KQD82_003350"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG4381799.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENLYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3054..4025
                     /locus_tag="KQD82_003351"
     CDS             3054..4025
                     /locus_tag="KQD82_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG4381800.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4281..4883)
                     /locus_tag="KQD82_003352"
     CDS             complement(4281..4883)
                     /locus_tag="KQD82_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG4381801.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEDSRQFAKQIYYILDNSIEVDNC"
     gene            5164..6375
                     /locus_tag="KQD82_003353"
     CDS             5164..6375
                     /locus_tag="KQD82_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG4381802.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNIKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6443..6640
                     /locus_tag="KQD82_003354"
     CDS             6443..6640
                     /locus_tag="KQD82_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG4381803.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAIFSPI"
     gene            6674..7645
                     /locus_tag="KQD82_003355"
     CDS             6674..7645
                     /locus_tag="KQD82_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4381804.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7832..8209
                     /locus_tag="KQD82_003356"
     CDS             7832..8209
                     /locus_tag="KQD82_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4381805.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3435 a          887 c         1351 g         2762 t
ORIGIN      
        1 taaattagag tatttatcaa ttcatagtag ataaaattga aattaaatat aacaacaaaa
       61 aatgaaaatt acaattaatt taatgttaaa gactcaatta tttggatgtg gaatggatag
      121 tacaagtagt atataaaaag tattgactgg acaaataact atattttatt ttaaatatat
      181 aatataatgt aataaaatat atcaaatgta gaaggtgata acctatgaaa aaaataacaa
      241 taaatgatat tgctaacttg gcaggagtat ctaaaagtac tgtatcaaga tatctaaaca
      301 ataaagatat cagtgattct actaaagaga aaataaagac aataatagat gaatatggat
      361 atgagccgaa tgcatttgct caaagtttga gagctaaaaa aacatacttt attggtatta
      421 taacaccgtg tttagattct tttgtaaaat ctaaaattat gatggcaata gatgaagaac
      481 tcaaagaatt aaaatataca tcactaatta ttaatactaa tagaaaaata aggtcagaaa
      541 tagatagtat atcaaagtta gctagtctaa aagttgatgg aataatacta attggtaccg
      601 aaataactaa agaacataag aatgaaattg aaaagttaga tataccgata gttgtagttg
      661 gacaaaaagt tgatggtatc aatagcatag tgaatgatga ttatggagca ggatataaaa
      721 tgggtcaata tattgctaat aaaggttaca aaaacattgt atatttaggt gttgatgaaa
      781 gtgatatatc agttggttta aatagaaaaa atggagtact taatgggtta aaagataaag
      841 ggtatgatgc aaaagtattt tatactgact ttgaccaaga aacgtctata caaagaagtg
      901 gagaaatgct agaaagtgaa aatccagata taataatatg tgcaactgat aatatagcaa
      961 tagcaacaat gaaagaaatc aataagagag gtaaaagtat accacaagat atatctgtag
     1021 ctggatttgg gggatatgat gtattgtcta ttataagccc taaacttaca acaataaagt
     1081 ttgaaaataa aaatgcaggt aaagtagcag caaatacgat agtaaactta atacaagaaa
     1141 gaaaagaacc actattaaag gagattaaat ttgaattaat agaaggtgaa agtacaataa
     1201 ataaaaatta agatacacat tacatatatt agtaatgtgt atttttttac taaaaaaact
     1261 ataattctaa aaattaggaa aacttttttg aaagaaatat tgcatctgaa ataaatttat
     1321 gctaatcttt aattaaaccg gtttcgaaac cggtttaatt aaaccgatag aatagcaaaa
     1381 tgagtgcaaa aataactgat tgtaacaaag tatcaaagta attattggag tacataggaa
     1441 taaacaaaat ataaaagatg tagcacattg tgtaataaga ctaagaattg ttttagatag
     1501 caataataaa gcaaatataa aattataaaa gaaaaggaat aaaaatatgt acaaagagcc
     1561 taaatataga accatattag aatcgacaaa agaagaatta aataaattaa gaaatatatc
     1621 tttaaatgat aaatataaac cactatttca tatacatcca caacatggac tacttaatga
     1681 cccaaatgga ttagcatatt ataatggtaa gtatcatgta ttttatcaat ggtatccata
     1741 tgatgcaact catggaatga agcattgggc ttatgtaagt tctgatgatt ttgtaaactg
     1801 gaatagagaa gatgtagcat taataccaat cgaaagttat gaatctcatg gagcatattc
     1861 aggtaatgcg atagaggtag atggaaaatt gcatatgtat tatacaggga atataaaata
     1921 tagtgcagaa gatagatatg catatcaaaa cttagctata atgaacaagg atggtaaaat
     1981 aactaaatat gaaaataatc caatagtaag tgaaatacca aaaggatata caggacatgt
     2041 aagagaccca aaagtattta aaagaaagga taaatacttt atgttacttg gagcacaaac
     2101 aagtgataaa aaaggagtta taatagtata tgaatctaaa aactctatag attggaattt
     2161 taaaggagaa ttaaatgtaa aaaacataga tgaagatttt ggttacatgt gggaatgtcc
     2221 agattatatt aatatagatg aaaaagatat attaatattt tcaccacaag gagtagaacc
     2281 taaaggattt gattatcaaa atatatataa tgtagtatat gctattggta atatggattt
     2341 agataattta acatttgaaa tagatacaat gaaagaactg gaaaaaggat ttgatttcta
     2401 tgctccacaa acatttataa aggactcaca aataatacta tttgcatggg ctggaatggg
     2461 agaagtttta taccctacag ataaaaacaa atgggcacat tgtctaactg ttccaagaaa
     2521 attaaatata aaaaataata agctattgca aatgcctgta gatgaattaa ttaaattaag
     2581 atatgatgaa acaagtgggc aaaatactat aaagaataat atcaatatta tagaaaataa
     2641 tgaaaattta tatgagctta atattaatat aaaaaatatt gacagcaata aatttgggct
     2701 ggaattattt tcatcacaag atgaaggtgt aaaattagaa tttaacaaat taggaaacat
     2761 tgtaacttta gacagaagca actttaaaaa agttttcggt gttgaatatg gaacaaatag
     2821 aaaagaatat ataaatattg atgaaaatac taatatcaaa gtactagcgg atagaagtat
     2881 tttagaaatt tttataaatg atggagaagt agtatttact agtagaatat ttgctaaaga
     2941 aaattctaat caaataaggg tttatagtga taaaatagta gagtatgagt atactaaatt
     3001 taaattaaag caaggtattg aattataaaa acacattata aggggtagaa aagatgaaaa
     3061 aagtaattag cataggagaa gctttaatag actttatacc gaatcaaaat ggtggtaaat
     3121 taaaaaatgt atcagaattt agaagagtag caggaggagc tccagcaaat gttagtgctg
     3181 ttgtagcaaa gcttggagga aaatcgagtt ttatatctaa gttaggaaaa gatgcatttg
     3241 gtgattatat aatagatgta ttaaatgaag taaatgtaaa tactgattat gtattgagaa
     3301 caagtaaagc aaatacagga cttgcttttg tatcattaaa agaagatggt aatcgtgatt
     3361 tctcatttta tagaaatcca agtgcagata tgcttttaga agctgatgaa gttaaaaaag
     3421 agtggtttaa taattgtcat atcctacatt tttgttcagt agatttaata gatagtccaa
     3481 tgaaattagc acataaaaaa gcaatagaat acgcacttga aagtaatagt ataataagct
     3541 ttgaccctaa tataagatta cctttatggg atagtgagca atcttgtaaa aaggcaatat
     3601 cagaattttt accttttgct catattgtta agatatctga tgaagaatta gaatttataa
     3661 caggagaaaa tgatatagaa aaatcattac ataaactctt tgtggggaat gttgaactag
     3721 tattatatac aaaaggaaaa gatggagtag atgcatacac taaaaaagta aaaggtatgt
     3781 gtaaaggggt taaagtaaat gcaatagata caaccggagc aggagattct tatataggtt
     3841 catttttata caccttattg tataaacaaa taacattgaa tgatataaaa gaaatgaaac
     3901 aagaaacgtt aaatgagtat ttagaatttt ctaattatta tgcagctcgt agtacaacta
     3961 ttaaaggtgc gataagttca tatgcaacaa aggaagaaat aactgaattt ataaaaaatt
     4021 tatagtagga atgagtaata ggttttgaag gaagtaacat gtaaagagtt tttaaggatg
     4081 tttactatga aaataattaa catataaaaa gcaataaaga ggctgtctca aaatagagat
     4141 aagcctcttt atcattgttg aaatgataaa aaatatttta attattgtta ctttttagaa
     4201 gaatatatat cttattttaa ataagcaaat tatgtaagat ttatttatat tatcaatact
     4261 ttacttggag tattatgtct ttagcagtta tctacttcaa ttgaattatc taaaatataa
     4321 taaatttgtt ttgcaaattg acgagaatct tcttcttttt ctttattcat aaaattaggt
     4381 ttacctaaat cacgttcaac aagcattcct atatatttta actgtgaatg cttacagtag
     4441 cgcttcatac cattttccca aatatcagca cccttttcag gtctatatcc acatgttgta
     4501 ataagtgcta ctggttttcc tgcccataga gcggggcctt tctcattacc atagtattta
     4561 ttcattccat acaccaatct gtcaagtagt gctttcattg gagaagtgca gtaccaagaa
     4621 taaattggag tagcaaaaac aattaaatca cacataagaa ttttcttgaa aattttctga
     4681 acatcatctt tacaataaca tccaaaatca aataagttat cctgacactt tttacaagca
     4741 atacatggct ctattttaca atcatataac catattactt caatatttga attataattt
     4801 tctaattcct caataaaagg ttttaaaata gcagcagtat tacctttttt cttaggactt
     4861 cccataagaa tgcatacttc catattgata cctccacagt attttaaaat agtttttagt
     4921 gcttaatata gttttaggat actatgagaa tacgttaaca acaatacact ttgtatattt
     4981 gtaataaaat tgaaacaaat tgttttatca ataaaagttg gaatgtatct ataattaaaa
     5041 atattttagg tatgttttta catatatgtg tgtagttaac tatggtaatt tattgtaaaa
     5101 ttatcttata ggattataat tgcttaattt atgattatat ttaaaatctt ggaggtaaaa
     5161 aatatgtact caaatgaaga ctataaattt atagaagaaa atgcaccatg ttatatatat
     5221 gatgagacta atataattga aaaatgtaag gaattgaaaa ataatatttt aggttttgat
     5281 tttttatatt ctataaaaac aaatccatat aacaaaattg taaaaagcat tgcagagcaa
     5341 ggttttggag cagatgcttc ttcaactaat gaagttatga tttcatcaaa atgtggagtt
     5401 tctaaagatg atatttatta ttcatcacca ggcaaaacag agattgatat agaaactgtt
     5461 ttagataaat gcataattat tgcagatagt attacagaaa tagaacttat aaataagata
     5521 tctaaaaagc ataatcagaa agttaaaata ggaataagaa taaatcctga ttttactatg
     5581 gatgatgata gtgctaaatc aagcaaattt ggtatagata tagaagatat gagcagtgtt
     5641 tttaagatgt taagtatgtg tgattatgta tctgtagttg gtattcatat acatattaaa
     5701 tctcaggtat tggattttga aaagttagga cattattatg aaaaatgttt ttttatagct
     5761 ttaaatttaa ataaaagtaa gtatattaat attgaattta taaattttgg tagtggactt
     5821 ggagtagctt atgataatct tagagaaaat ccacttaatc ttaaagagct ttcaaaatta
     5881 actcaaaaga ttgcttttac taattcaaac acactaaaat caagacttct tattgaatca
     5941 ggacgtttca tagtttgtaa atcaggtaat tattatgtta aaattgtaga tattaaagta
     6001 tcaagaggga aaaaatatct tattgtaaaa aatggactta atggatttat gagacctatt
     6061 atttctaatt taattgaaaa tattacttgt ggagtagaat tagaaggtca agagccatta
     6121 tatacatcaa atcatgcctt ttctctttca gtattaaata atttaaaaga agaagagtat
     6181 gtaaatgttg ttgggaatct ctgtacatca ttagatgtaa ttgcaaataa tattaaattg
     6241 aaaaaagcaa acataggaga tttgatttca attagtaatg ctggaagtta ctcctattct
     6301 ttatcaccac tattattttc gagttatgaa tttcctaaac aatattataa aagaaaagat
     6361 ggatatattg agtaaacaat agcataaatt aataaactta gtattattga aaaaatttaa
     6421 aatgcagggg gtgtaattct atatggaatt gtggaaaaga aatttatttg tatgttggat
     6481 aggtatgttt tttagttcta tagggatgag ccaaatagca cctatacttc cattatatat
     6541 taaacagttg ggggtaactg atgttagttt aatacaacag tattcaggta taatttttgg
     6601 atgtaccttt gtagtagcag caatcttttc gccaatttga gggaaagctg ctgataaata
     6661 tggaagaaaa tcaatgcttt tacgtgcaag tattgggatg ggaatagtta tatttaccat
     6721 ggcgtttgtt caaaatgtct atcagttatt aggattaaga atacttcaag gagtttttac
     6781 gggttatgct actgcatgta ctactcttat tgcaacacag acagataaaa atcattcagg
     6841 atgggctttg ggtactcttg caactgcatc aactactggc tcattaattg gtccaacagt
     6901 aggaggatat atagaatcaa tcttaggtct taaaagtaca tttattataa caggaggatt
     6961 gttgtttgta tcatttatca tctctatttt atttgtagta gataatttta aacctaaaga
     7021 aataaaagaa tcaatttcta tacctaaaga acaattaaat atattgccaa ataaattttt
     7081 aattgcatct atttttgtaa ccacattcat tactcaacta gctttatact ctatagaacc
     7141 aattgtaacc atatatatat cacaacttac aaattttgca tcaaatgtag ctttaatagc
     7201 tggattgaca ttttctgcat caggattagc aaatttatta gcagctcaaa aacttggaaa
     7261 aatgtcagat aaaataggcc ctcaaaaagt attgttaatt tcacttttat gggctgggat
     7321 tatatttata ccacaagctt ttgtaaaaac tgcatggcaa ctgatgttgt tgagattttt
     7381 attagggttg tcagttgcag ggcttaatcc ttctgttaac agcttgctta aaaaaattgc
     7441 accagaagaa tatgtgggaa aaatatttgg atataatgca tctgcccagt atataggctg
     7501 ttcatcagga gcatttttag ggggacaaat atcagcacat ttaggtatta gaacagtatt
     7561 tttttcaaca agtttattgt tgtttatgaa tgcactttgg atgtataaat ttactggatt
     7621 gtttattaaa gataagacta aataatcata ctatttacat ttctaatttt atgatgtaaa
     7681 ataaatattt tttatgtaat ttatcataaa attatataaa aaataaccct atggtaaaaa
     7741 ttggaaaata ttattatcga caaaatcttt gacttaaaaa ctatattgcg atagtattaa
     7801 tttaataaaa gtttgagagg ggcgtaaggt tatgacaatt tcaaaaatac cacaagcaga
     7861 attaaaagtt atgaagttta tttgggaaaa aaatgataca gtaacatcaa aagaaactat
     7921 tgaagctatg gaacgaaaat atggttggaa acaaacaact acattgacac ttttatcaag
     7981 acttgttaaa aaacattttt taagtgcaga aaaaatagat agatacactc attatacaat
     8041 agttgttgga cataaggagt atttaagtgt tgagacaaaa gattttttaa gcaatataca
     8101 cgacaattct ttacagagtt taatttcagc actacatgat gatgaagttg ttgataaaga
     8161 tatgttggat ttatttgaaa gtcattttaa aaatttagaa gaagagtaat tatatcagga
     8221 tagaactcta agaatttaat taatctatgg ctgaaaacca taaatggtat tatttaatca
     8281 gctactcact attcataaag taatattaaa taggttctct aattagtaaa atgcctaaaa
     8341 taattggaat agggagtatc aagttaaatg ataccactta tttaaataag aaatgatttt
     8401 taaaatttaa aataatttta taacttgtca atatt
//
LOCUS       DAENCV010000154         8400 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAENCV010000154 DAENCV010000000
VERSION     DAENCV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.157"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(208..1077)
                     /locus_tag="KQD82_003357"
     CDS             complement(208..1077)
                     /locus_tag="KQD82_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4381806.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1174..2031)
                     /locus_tag="KQD82_003358"
     CDS             complement(1174..2031)
                     /locus_tag="KQD82_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381807.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2246..4264)
                     /locus_tag="KQD82_003359"
     CDS             complement(2246..4264)
                     /locus_tag="KQD82_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG4381808.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4535..5311)
                     /locus_tag="KQD82_003360"
     CDS             complement(4535..5311)
                     /locus_tag="KQD82_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG4381809.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5368..6339)
                     /gene="asrC"
                     /locus_tag="KQD82_003361"
     CDS             complement(5368..6339)
                     /gene="asrC"
                     /locus_tag="KQD82_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG4381810.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEDINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKKPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6352..7143)
                     /gene="asrB"
                     /locus_tag="KQD82_003362"
     CDS             complement(6352..7143)
                     /gene="asrB"
                     /locus_tag="KQD82_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG4381811.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7144..8151)
                     /gene="asrA"
                     /locus_tag="KQD82_003363"
     CDS             complement(7144..8151)
                     /gene="asrA"
                     /locus_tag="KQD82_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG4381812.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDLKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2713 a         1516 c          754 g         3417 t
ORIGIN      
        1 attcctgttt ttattataat aggtaaaatt ctattaaact agttcacatt ttcattatta
       61 ttgatatatt atatatgaat ataccatttt gttttttaat aaaaaaatca tgctacatca
      121 atagtaagca actattaatg tagcatgatt ttttgtatct aaataatctt agtatttacc
      181 tatcaatata gattgatata tcaaaaacta tagtcttcta tattcttctc tttttagctt
      241 ttggttactt agatagtaat caatttcatc aattaaacgt tgtttatcat caggtaatac
      301 accttttaaa ttagcataat ttgatgcatg attacttcta aatacaattt tttcattagc
      361 atctatattc tttatcatct ctttaatttc ttgtaacaca gccttatcct ctagtattgt
      421 aaactctcct gccttaagta aatcgtaaag ttcagtatta ggttctacca ttaaactaag
      481 tacacccaca taatctggtg aaattgctcc taacattttt cctgtatcaa ctgcatgctg
      541 atgtgttttt ttagttcctc caagacctgc tattacagtg gctgataatt taatcccact
      601 ttttttaact tttctaccaa cttttattaa ttgttcacta ctaaaacctt tcttaatatc
      661 ttctaatact tcattgcttc cactttcaag ccctagataa atcattttaa caccaagttt
      721 tcttaattct ttaagttcat catcagattt tttctctata gatttaggag agccgtacat
      781 agtaattctt tcacactctg gaaaaacttc ttttatatat aaaataatat ctcttaattt
      841 ctccattggt attatcaatg catctccatc tgctaagaat attctttcta catgcttata
      901 atatcttcta aatatatcaa tttcattttt tattacttct agtggcttta ttttaaattt
      961 tttagatttg tacatactac aaaacgtaca tttgttatga gaacatccta gtgtgacttg
     1021 tattataaga ctataagcct cacttggtgg tctgtaaagt ggcatatcgt attccatatt
     1081 aaaatccccc ttgtaataaa atattcattc tattatatta ttaatattta ctttaaataa
     1141 taacttataa aattatattt tttaaatttt agtttaagat atattcttta aaaatttaat
     1201 gatactattg gcagtaatat taagttgttt ttcttgactt ataatagcat cattatctcc
     1261 cttttgtttt ccataatctc caaattgtgc atgatttcct ccttcaatct caacataagt
     1321 tgtatcatca ggaagttttt gttttgattc tacaagactt ttaaaattga ctactccatc
     1381 tttgctcccc catatagata taactttttt tcctaattct ttcaactcat cacccattgg
     1441 ataagaagct aaaaatacaa cgccttcaat taatttatta tcacgtgcaa aatttgatgc
     1501 tgcaactcct cctaatgaat gacctcctat tacccaatgt tctatgttgt tgtaagaatc
     1561 cataactttc tgtgccttat tagttccaag tatagctaga ttaagtggca tctttacgat
     1621 tacaacctca tatccacttt ctgctatttt tcttgcaagt ggagcataag attctatttc
     1681 tactttagct cctggataca aaattaaacc tttagaaact tttcttcctt ttggtgtaaa
     1741 tgaaatgttt tcatttacag ttacttcaac tttactattg ctgactagag cttcttttgc
     1801 cagcttttga ggttcatatg ttttatttat ccatatgaaa aagccaagca aaatcaatac
     1861 aaatagtata cctattccct taaatatctt attctttatg ttaaattttc tattattatt
     1921 tttatcatct ttgttctcaa cctcatcata aagtaaaggt ctctcaagtt ccatagttat
     1981 actaaatgta tcatatttct tattacgctt tacctttaca tctttactca taaatctaac
     2041 ccccataata tttgaatact attatagttt atcctaagta aattgttttt tcaaccaatg
     2101 aaaaaaatca atattttaac tattttcata taaaattcat aaatatatgt tttttgtttt
     2161 ttatatatta ttttctttta gtattttata aaacaaatag ccatatactt tattttaaat
     2221 atatggctat tctataattt aatatctaag ctgcttcttt tattgaaact ttagttatat
     2281 tacttattct atacatttct ttgacaccat ttagagtttg cagtaagttt agacatatta
     2341 tcttgtcacc tagactttta gtatttacaa tactctctaa aattacactt cttccttcat
     2401 catacttatc tacattgtaa tttgaaatat aattagtatc tctcaatatt ttttcaacat
     2461 cttcatcttt tattttattt ttattaacat ttatatatag attatacata ttacttatta
     2521 gtaatttctg attttctaaa aaatcctctt gatgtttatc tttatatata gcatttatca
     2581 acataagtct atcttcaata agtgatggta tcaataataa attttttata tagtaaacat
     2641 cagctacatt ttctatagac ttattgactt catcaattat atttttaact attcctttat
     2701 acatttctat aacatgttga tagcctgttt tagcatcata tggtaatata aacttattta
     2761 ctattaatgc aataattgct cctaatgcta catatgaaat cctgcttatg gttaaaacat
     2821 ttgggtctcc cataactact gctgaaccaa gagctgaaac agttacacat accattaatt
     2881 ttctataatc aaccacataa gagtttatat accctgctaa aagaactatt aacgacctta
     2941 atgtactatc ttttattatt gaaaataata caataaatat tacagctcct ataaatgtac
     3001 cttctattct ttttctagac ctttgtatac aattttctgc gtaaggttgt gttaatgaaa
     3061 agacagttaa cattatccat cttccatctc ttaaatggaa ataatccata ataaaacccg
     3121 ctacagctgt agctaaagca atctttactg cataagaaaa tcttatagaa tttaaattaa
     3181 agtttttctt atatattgat agtcgtttaa attcatgagg tatctttgat tttctttcag
     3241 ctttcttttc gtctcttgat tttacttttt tataatctac taaaaactca tataaatttt
     3301 ctaaaacact ataaatttct tttaaattta tatttttagt atcctgagca ataacataat
     3361 ctctaaatcc taatatctct ttatcagtta tggtatcttt attttctaaa cacatcttaa
     3421 tatagtttat ttctttcgtt gctacaacta aaacatctga tttactattt atgctacttt
     3481 cttctaaaat attttttata tcttcatctt caaattcttt actatccttt ttgtatcttt
     3541 ccaggattaa acttattctt tctaatgaaa ataaaatatt cattatgtct atacctctaa
     3601 tactgatata aaagtcatct tttctattat caaaaataac cattttcaaa tttttagata
     3661 tagaatttat tctataatta cagctatcta tttttctcat actagattta attatagaac
     3721 tatcatcatt aatagtattt tttaaaagac taattttttc taataattca tcacatatag
     3781 atatcagttc tttatctccg acatttttca tcctatttct attcataaca aattgagcaa
     3841 tcattattat aactgctcca acagctaaag ccgacaatct cataggaaat tcactttttc
     3901 ctacaggtat acttaccata aatatataca ttagaccaaa aggtacatat actgctcttt
     3961 ttaagtcata actaaataca taccctatta taaataatgc tataaaattt aataaaactc
     4021 ctaaatacat attttgtact gcaaaaaatg atagtattcc taagattata tttattgcta
     4081 aatacttcag taaattttta attggagaaa tagttaaatc tctttccaag agtgttagca
     4141 tagctgttac tatacctaca cctataagtg tatttttact tccaaataaa tatataaata
     4201 tagaaataaa tgctattata aaaataaaca actttgtttt tgatattact agtttctttg
     4261 tcatcatatc ccccctaaaa atttcaacaa gaccaatttt atcatatttg ttatcttatt
     4321 caaaatgcgc ttttttacgg taggacttta acttttttta tatttagaac tatataattc
     4381 atatataaac gtatttttta tatatataac cgatatattt tcgtatgctc ccatattttt
     4441 tttgtttcaa atattttttc aaaaatataa aaataactct ctcaataata ctaaaaatgc
     4501 atttatcaag agagttataa tttatacaat aaatttaatt aagcttttct ttacctaata
     4561 tataagttcc aagacccata aataaagctc ctccaacaat atttccaagt gttacagcta
     4621 ctaaattata aatagctcct ccaatagtaa ctgtacttat tgttggtgaa aataatgaaa
     4681 cactataaat tgtcatattt gctacactat gctcaaagcc tgatgttata aaagcaaata
     4741 aacataagaa tatcataatt atttttgcag tatcactatt agttctaaac gaacacaaca
     4801 ctgatacaca tactagaata ttacataaaa tccctctaaa gaaaagagct gtaaatggca
     4861 tggatgcttt actagctgct gtatttgcaa aaaattcagc tacaggtcct ttgtctacta
     4921 gaccagttcc tacaaatata atgcctaaaa ccaaagctcc gattaaatta cctacccaac
     4981 tgtatgccca tattttagaa gtatctttta tacttacacc tttatttaac ataccagcag
     5041 acataaccat attgtttcca gtaaataact ctgttcccgt cattataacc aaacttagtg
     5101 ctattgcaaa tgataacccc attactatct tagtcatagg tgaacctgca tcagtcaaaa
     5161 gtcctccaat agtaaatatc aaaagtatac ctatacctac ataaagtcct gcaaacgcag
     5221 aagataccaa atattttact ttacttgtgt ttaataaatt tatcttattt atagctgcat
     5281 tagttaattt atccaaagtt tctttatgca taattccctc ttccttctat taaattttaa
     5341 tttattcaaa tatatgtttt actcaattta tagatattta attccactcc agtatatgtt
     5401 ttcgtacatt ttagttattt ctggaagctc tacaccttca agtgcccatt ttttaaattc
     5461 cataaatcct gttctatcaa ttatatatcc tatatgttct tttcctcctg gtgcatcttt
     5521 atctatatac ttttctacat atctataagt attaagtatt atctttatta tgctgtcctc
     5581 atctacccat aaaagaaaat cttctgctag tctaggtttt ttctttcctg ttctacccat
     5641 tatagctaat ctatagtatt ttttctcatc tctagtccat gcatttgttg ggcaatttag
     5701 tacacactct ccacagccta tacacttatc tttatctctt accaccttat tattttctgt
     5761 ttttaatgct ccaacagata tcttagaaca tttttttaca catgctccac aatttacaca
     5821 tctatccttt tcatatattg gtaaagccat accaataatt ccaaagtcat tcattctagc
     5881 ttttatacaa tcatttggac aaccagttaa agccacttta aaatgtaaat catttggaaa
     5941 tatagctttt tctattcttt tagcaaattc agttgtatta tattgagcct ttggacagac
     6001 tttatttcct atacaagctg ctatatttct tgtacctgcc gctggataac ctgcgttttt
     6061 ctcactttga tttatattca ttttttcaat aacaggttgt ataattttat ttatatcttc
     6121 catatcttcc atatttattc ctaatatttc aaacccctgt cttgtagtta tgtgcacctg
     6181 tccattgccg tactcacttg caattttaga aactatagct aaactttcag ggtcaataag
     6241 accaccaggt actctaactc ttaaagaagt ctcatatttt gtttttgtaa ttctataagc
     6301 atttttcatt acttttttgg tatttaaatc tcttatcaca aacttcaccc cctaatcaat
     6361 aagattttta gcatatgaat aatcaaatac tggtccatct aaacatatgt aagtatcatc
     6421 cattttacaa tgtccacatt tacctattcc acaacacatt tttctttcat aagaaaccca
     6481 aatgttcttt tcatctaaat ctctttttaa aaattcttct actgtaaatt tcatcatcat
     6541 tggaggtcca actacaacta tagaagtatt gtttatatct ttcatatcca attctggtat
     6601 gtattttgtt actaatccta cattaccact atatccttcc tctgctccat ctacagtaac
     6661 taaaatatca agttttttac tccatctctt taaatcatct gcaaataaaa tatcttttgg
     6721 tgatttaaat cctactatta atttaaactt ttcgcatttt tctttattat tataaaaata
     6781 ttcaactatt cctcttacag gggctaatgc acttcctcct gccactacta caatttctct
     6841 tccttcataa agggatacat caaatccatt tccataagga cctcttataa aaaaagaatc
     6901 tccttcttta taattaaaca actcgctagt tacttttcct acatttctta ttgtaaagtc
     6961 aatataatcc tctccatatc ctgatactga tattggactt tcaccgtatt taggtataga
     7021 tatctcataa aactttccag gcaatacacc ttttgtattg cagtttactc taaaagtcca
     7081 ttctttatca gtatgtttaa ctatatctag tatttttgca cttactggta tatatgggtt
     7141 catttatact tcctccttac taattactaa atcatttatt ttttctatac actttgaaaa
     7201 tgatatgtat tgcggacagg catcatcaca tcttccacag ccaacacaca tatgatatcc
     7261 gaatcttttt ttaaaatcat gtattttatg catagtttta aatctcattc tttcgccttg
     7321 tttttttcta aaagaatttc cacctgctat atctgtatat ccatctactt gacaagaagc
     7381 ccaaaccctt cttctctcac ctacattttc gttttcctta tagaaaatat cctgcattgt
     7441 aaaacatgaa catgttggac atacaaaatt acatctacca catcctatac atcttgtatc
     7501 atattcatcc catattgaat ttcctatcat ttcatttgaa tcaatattct ctggcacatc
     7561 tacagatatt aaattatcag ttacataatc aacattaaat tcagttgctt ctccattaaa
     7621 tacttcaaat tcactatcct ttaaatctaa gtacagtgta tctccttcta tatttaatcc
     7681 aattgaataa ttatcagttt tgttagaatc catgctcaca caaaaacagt ttctaaaact
     7741 ttccttacac cctacaagtg caaactttac tttatctctg atatttttat aaaaataatc
     7801 tttttcttca ccattattta gatatatttc atcaagtctt tttataccat ttatgtcaca
     7861 agctcttaag aaaattaaaa ttttcttgtt gtctaaatcg ctttccttaa actctttttc
     7921 agtaaaataa aacaatactt gatttatggg aagtacaact tcttttggtg aaaagtttgc
     7981 tttcttagca aattccattt cttcaaaagc gtttacctcc ttgtatctta taacatcagt
     8041 atctgaaaaa gtacctttaa atgggattaa aactggtgca aatatcttgt agtctttttt
     8101 tagataattc agaccttcat tgaacttaga tgaatctaat tttagtttca tttttatcac
     8161 ctttcttata ttttagtttt acttacttgc tatcttgttt aaatcttaac actttatttt
     8221 ctatgcttcc gtgataatta tcacagtttc aaaaataata aaaaaaatat taatggagtt
     8281 tattaagttc tgttaatatt tttttattat ctacattatt caatttcaaa tctaatcact
     8341 taaatacaat cattaagtac aataaaacta ctatctactc aattttttga ataaaaaaga
//
LOCUS       DAENCV010000155         8048 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAENCV010000155 DAENCV010000000
VERSION     DAENCV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.158"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(81..713)
                     /locus_tag="KQD82_003364"
     CDS             complement(81..713)
                     /locus_tag="KQD82_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG4381813.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(785..1138)
                     /locus_tag="KQD82_003365"
     CDS             complement(785..1138)
                     /locus_tag="KQD82_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG4381814.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1216..1419)
                     /locus_tag="KQD82_003366"
     CDS             complement(1216..1419)
                     /locus_tag="KQD82_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG4381815.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1809..2039)
                     /locus_tag="KQD82_003367"
     CDS             complement(1809..2039)
                     /locus_tag="KQD82_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381816.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2041..2448)
                     /locus_tag="KQD82_003368"
     CDS             complement(2041..2448)
                     /locus_tag="KQD82_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4381817.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2808..3257)
                     /locus_tag="KQD82_003369"
     CDS             complement(2808..3257)
                     /locus_tag="KQD82_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381818.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3271..4197)
                     /locus_tag="KQD82_003370"
     CDS             complement(3271..4197)
                     /locus_tag="KQD82_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381819.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4322..5104)
                     /locus_tag="KQD82_003371"
     CDS             complement(4322..5104)
                     /locus_tag="KQD82_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG4381820.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5260..5880)
                     /locus_tag="KQD82_003372"
     CDS             complement(5260..5880)
                     /locus_tag="KQD82_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG4381821.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5968..6531)
                     /locus_tag="KQD82_003373"
                     /pseudo
     CDS             complement(5968..6531)
                     /locus_tag="KQD82_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6767..7582
                     /locus_tag="KQD82_003374"
     CDS             6767..7582
                     /locus_tag="KQD82_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4381822.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIGNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PYIFMHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
BASE COUNT         2626 a         1382 c         1175 g         2865 t
ORIGIN      
        1 taaaataata tacaaatttt gaaaaagctc ttataaaatg attattaaag taaagtttaa
       61 tataaataaa ttataaaata ttaaactaat ttatcaatat ttcctgattg aatatgctta
      121 atgtttgatt gaattttttt atatggccaa tcccaccatt taatttctaa taattttgaa
      181 attgtatcat tattaaatct cttttttata atttttgcag gtactcctcc aacaatagaa
      241 tacggtggga cgtcattagt aactactgct cttgttccaa taattgcacc atcaccaata
      301 tttacacctg acataattat agaatcataa cctatccaca catcatttcc aattataatg
      361 ttacccttat tgtcccaagc gtccgttata tggttgatat cgagtttcca ttcttcataa
      421 aagatgggaa aagtatatgt ggattttgat ttcattgagt gatttccact agtcattaga
      481 aattttgcat tacatgctat tgagcaaaac ttaccaatta ttaatttatc attgtttata
      541 gggtaatgat acaatacatt gtttttctca aaatctcttg ggtcattata aaagtcatta
      601 tatattgtgt aatctccaac ttctatattt tcccttgtta ttacattttt aagatataca
      661 gtttgataat cattacttct tggatatacc ttatttgaac ttggaatagt catatttaaa
      721 tctctccttt aatatatatt atgtttttga ctattccact actacaatac atctcttcac
      781 agaatcactc cttgttaaga atcttcataa gataatggcg tcctttttta gtgttaagtc
      841 ggatattctt tcttagtaca ttctttttag cgtataactg acaaactgtc attttcttac
      901 caatagtgtc aatgctatca tcaaggtcat tttgtattgc tttttcctta tcccttaatg
      961 atatatcatc acgttttccc tttgggggtt tatcagtagg tacaagtttc caagagtaaa
     1021 caaattgtgg tttaccaaat gaatatacat acttatatgc gtatcttcca tcttttctct
     1081 ggctctctcc agttcttaaa attctatctt tattatctcg tcttttttca gttgacatga
     1141 taagaagctc ctctcaataa ccgaattgag cttgatacgt catatgaata ttataacata
     1201 taaggctcaa tttcattaga taacgtcaag tgtatcaacg attttctcaa attgttttct
     1261 ctttatttga atgcgattgc tattcataat gacccagcca gaatcaaggt tttcatcagc
     1321 aagtttacgt aattttttct ctccaatacg aaagtatttt gacgcttctt caatcgtcaa
     1381 ggtatatttt tcccaaatag gtacatcagt attgttcata gaatgtatca ctcctttcta
     1441 tgttcgtagt tcagaagtag atagatggct ttggtaagct ccattggtat ttcaaccaat
     1501 cccattctta tgggcagaac cgtgtcatac aggtgtatca ttatactgtg gtacaggtta
     1561 cggcaaaaca accgttccaa aggttattca caaatcacta tatagtagct ctcttgatta
     1621 aggtcatggc gtatagtatt tgttggctat ctgtatcaca aacgtatcta tctactttat
     1681 tcagttgtca aagaacagtc ggcattgatt tgcctataaa aaatcacaat agcttgtggg
     1741 cttttataga caaataatta gtgattctcc atgtaccaaa tagcttgata catggagaga
     1801 gggcgagatt aagacaaatt aaatctcaaa atagcagtga ttagttttgt tcctagcatt
     1861 cgctttgttt cttcttctac atgaatatag ggatttccgt attcatcaaa taaagttttc
     1921 tgtgaaagac gaatgatata gtgttcaaaa tgtttaagta ttccgtttat tgcatttaca
     1981 tcgccatgta cagcaagtaa aataattgta aaaggtaata cttctttagt aggtttcatt
     2041 tcaatgttcc tccatgagtt ctttcaactc ttttagtgca tttgttttgt gataatgtat
     2101 ggtattttct gctaaatcca tcaatgttgc tatgtcagca tttgtcatgt tgagaaagaa
     2161 tgaaagtaga attatatctt gttttctttt tgtaagtttt ccgattgctt gtgccattaa
     2221 tacatcttca attcgtatgt tgtagtcgta agaaaccata cagatgtagt tagaatcata
     2281 atcatctaaa atactcaatt tacttaattc tgcaagtgac aaagattcga gagataccaa
     2341 ataatcattt ctgcgtttat tttcgtcata aatatctcta gcatagttac gcaaaactgt
     2401 tttacagaaa ctgtcgaatt gatgttcaat tgtcttttca tcaaccataa gatttcaccc
     2461 tctttccatc gcaaggagtt aaataacctt gcttactcct atcacggtgg gagggaatta
     2521 aatcttcgat aaatttcaaa aaaaagagaa aaaaatacac actctgccta tagggcaaaa
     2581 atgtgtattc tttaaaacgt atttaattta tattggttgt aagccaccac catattgatg
     2641 tatggaaata tgcattggct agcttttcat aaaaatacaa agatgcataa gaaattaata
     2701 gaggggaaaa acttattctg ttattaaaaa gtaggttaat tctaataaat gctattgcgg
     2761 tcgaattaaa ctatatacat ggcaatcaat catgtttcta ttctttatta tgaattgtga
     2821 cagtattctt caatcttaaa accacatttt tccaatactt tacttgaccc tctattgtag
     2881 gaatatacat tagcatgaat tttgttaagg ttgtattttt caaaggcaat tttgcatatt
     2941 tcttttattg catttgttgc tatccctttg ccccaatagt tcctagcaag ccagtaacct
     3001 aattcagcat ttttattaat agaggtatct tcttcaaatg ttaggctgat acttccaata
     3061 gccctttctt gataaaaaat agcatagcta agttgtttaa catctggagt tgccaaacaa
     3121 aaactaataa aatctctagc gttttgctcc ctataagggt gggggaagtt gtcacgaaga
     3181 ttgtttgcaa tttctttatc attcgcaaac tcaactaact cggtaatata agcataattc
     3241 cactctataa gttccatttt tcttctcctt ttatgaaaca gctacaagaa atctttctaa
     3301 gccaatacca cttatcaaac agcgttcttt tttatttcca agcatttgat aagtccagtc
     3361 aacaaatcca ccgtcagcta tttcaacggt ttcatttcct tgtttgaccg atattttgaa
     3421 attgatacct ttataataat tattatcaac atcatcatta tgaattgata actctatatt
     3481 aggaaaaata agtttgatag tttcaaccat tctgtcgaaa aatccgtcat tatctttgta
     3541 tccgtttctt ttgcgtagaa taatagagaa acgaacattt tctatttcac ttaatatatc
     3601 tttataatag tttaagtgct tttctaacaa cattttttca caagtgtaag accctgtatc
     3661 tataccagat gaaaccatac aatataagcc aaaatgtgaa tataaacctt tacctacaaa
     3721 attttgtgct cttattaccc tagcagtagt agccatgtgg ataggtatgg tattattttc
     3781 ttcttttctt tttaatttat cagcaataat tattgccaac atatttgagg ggtctgcaag
     3841 tgtttctgtc cctcgtagag cacttactac gttatattga tctacggagc caaaaacgct
     3901 acagctacct aatggggcag agggagaaag cataatcgtc ttgatatcca tattgaatgc
     3961 ttttctcaat aaatttgatt ccaaagcatg aatttcttct ggatctatac cgctagggat
     4021 agtaaatcta tttagttggt atgactttag aatatcagtt gcagttaaag tatttgcttg
     4081 tctatcaaac acttctaata gaagtgagtt caaatctgat tttgatagag ctgataactt
     4141 atcaactata ttcttatccc caattttatt tgtaattcta cttaaaatat cactcatagt
     4201 tatcctccat actgttactt tcaaaataat tatagaattg ttacaacaac taatctaggt
     4261 catttgacca aaaatgaaaa aacactgaat atttactatt caatattttt ttactttggg
     4321 tttatttgat taaatcttga aaatactgta atgcaaccct ttcagcttca acctttcgga
     4381 caataggact ttgtgataat tcctctctta actcaagata caggttgata gtagcctgca
     4441 atgcattaac acagctttta tattcatggt tagcaaccgt cttttctaat gcttcaatat
     4501 attggggtgc gtccaattca atttttctaa ctccacgagg taatttacct aagagaagtt
     4561 gtattaatgg ggcaagtact gattgtctta aaaaagataa aaaatcaatt gtttcaaata
     4621 gttctctacg tccaatctta gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa
     4681 tccactgtaa gtctggagtt gggaagtatg cttctttttt agaataaatt tctgttatgc
     4741 agttgttttc ttcatagaga atagctgaat cttcaaccct atctagtaaa ccatctaagg
     4801 ttgtaaactt aaaatcaaca tgaagaacag gggaatcata gagggaaatt atcaatcgag
     4861 gttctccaac atgttcgcca gtaaaagcag aaactaatgt tccaaactgt tccacaataa
     4921 gtaatctctc ttcgataact tcttcatagg atttatcatc tgctacaatc agaaaatcca
     4981 aatcagaata ttcatctaac gattttgtga tataagaacc gctaattgca agtcctatta
     5041 tcctactatc tctttttgaa aattctaata cgttttgtat cattttctgt tgtgttttag
     5101 tcataatata acacctctca ctgtctttta ttagctatca atccaacata attatgcata
     5161 ggagtattat ataatgaaga aataaaaaaa aataggtcac ttgacccgaa ataaaaaagc
     5221 attgaataat tattattcaa tacttttttg aaattatact tatgttgtag gaagattctt
     5281 taatacctct cgctgattaa ttacactaaa tcttttgttt tcataagcaa ttagatgttc
     5341 ttgtacacat tcatttatag cacgattaat gcttctaatt ggagcgttta cttcgctagc
     5401 aagctgtttt ttggtaagca gatttaattc acttctataa tagtgtaagg atatacacct
     5461 cagaagtcgt tctttaacac ttaaagatga taattcttca attaattcag catttacaac
     5521 taatttacta gatatttctc gaatcagaaa ttttgtagct tcaaaatctc cccttaacca
     5581 gtttaaaaaa tcatctctat gaagtttata tacattacaa tctgtgaaag cgacaatttc
     5641 aacgggtttt ttcccatcat taaattgttc catttctcca aaaaaactac cacttctata
     5701 aagatgaatc gttgagatgt ttcctttcac atttaagatg aatgcttctg caataccgtc
     5761 aatcataaga attacatatt cattttcttg ctcagcaaaa agaatagtct ctttcttttt
     5821 atacgttttt ttaacaagct tctttttaat atcttcattc ataaaatcaa ttaaatgcat
     5881 attcatccct ccttaccaaa tctttgatta aattatatca tctttcaatt agttaggtaa
     5941 gatagtaaaa atatatttac atagaaacta ttccattatt attttccagt tgtcattttt
     6001 ctgcaaaaac ttatcactgt cacgactgga gataccagta agaaatgtta ggatatcaat
     6061 cgctaaactt tcagaatcct ttttacggct caaaatgacg taatggtcaa gcaatccttt
     6121 gttttcatca tcactggtta aattgacaat gtttatctca tgggctattt ctggtagcgt
     6181 ttctttccat ttgccgcgtt ttgctttagg gtcaactatg accgcctatc cgccgaatag
     6241 taccgcatag taaacaagca tattgttact gaataattta ccaccaccca atgaaccgat
     6301 aaacgcagac gctaacgcat tggtaataga accctttacc ccttgactag caagggcagg
     6361 ttttagatag acattctttc cagtgtccag attgtagcta acatagatat cctcatgttc
     6421 gccaagggtt tgtgtcgctc caaaaccaag tccagccaaa aaatccgaag tgacatattg
     6481 aatataatca ttgatgtaac gtttgctgac aggaataaat tcgccatgca atcccaacat
     6541 atccccaaag ggatgcacga gcttgattga taagtcgtcg taaaagtctt tgacttcatc
     6601 acaatgcctt tttagctcgt caagatcctt tgtagataca cggacaacat agctaagttt
     6661 atacatagat tctttacagt taaaagtctg tttttaaaaa aatgtataaa ttataaatca
     6721 aaatatagat actatcaatg acccactagg tgcaaaaagc attgatatgg ttaaattatc
     6781 agacgctaaa agctgggcta tccgaatgag tgaagatggg tgtgcttata aatccattgg
     6841 caattataag cgctcactga aagcgtcgtt tttcatggca attcaagacg actgtattag
     6901 aaagaatcca tttaattttc agttaagtga tgttttggaa gatacaacag aaccaaaaat
     6961 cgctctaaca ccagaacagg aaatgggctt attatccttt atggagagcg ataagactta
     7021 ttgtaaatat tatgatgatg ttgttatcct cttagaaaca ggactacgca tttctgaatt
     7081 atgcggtttg actttagaat tggatatgaa aaataaagcc attatggtag accatcaatt
     7141 gttgagtgat actaagattg gctattatgt agaagttcca aagaccacac agggtaagcg
     7201 agaaattcca atgactgaaa gagcttatca atcatttgaa cgtatcgttg aacggcgtaa
     7261 gaacgctaaa gcagaaccaa tcattgttga tggttaccag aacttcctat ttcttaaaca
     7321 agatggatta ccaaaacaag caactcatta tcaaagcatg ttaagagcgt tagtcaagaa
     7381 gtataacaag acccatgagg acaagctacc aaagattacg ccatatatct ttatgcatac
     7441 actctgtaca aatatggcta acaaaggaat gagtcctaat atattgcaat atattatggg
     7501 gcataaaaac attactatga cgcttggcta ttatgcacat ggttcattta gttctgcgag
     7561 agctgaaatg gaacggttat aagcctagta gtatgactag agtttactac ttatttacta
     7621 cttttgatgg cattcttata cggttaaatg ccaacttatg caaagtatct tccaaaaaga
     7681 aaatgtcgat actccttgta gatacaggat atatcgacat ttaccaactt atgaaaagat
     7741 aatcaaattt aatataagtt tttcaaataa aaagatacta aattcttttt cgagaatcta
     7801 tataacatta cttaagcttg gttggggtat aaacaattga gtagctactt ttgtatggtg
     7861 ttcaacttta gtcaaaactc ttaaataatg caaatgattt agattcatta atattcaatc
     7921 tcttcataag tagtgaaatt cgagttattt ctgcataatt atactatgat ttatagatta
     7981 attctattag catccagaat aatattgttt atatgaataa aatttaaaga atattatgaa
     8041 aattatag
//
LOCUS       DAENCV010000156         8015 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAENCV010000156 DAENCV010000000
VERSION     DAENCV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.159"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            89..763
                     /locus_tag="KQD82_003375"
     CDS             89..763
                     /locus_tag="KQD82_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381823.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            787..1791
                     /locus_tag="KQD82_003376"
     CDS             787..1791
                     /locus_tag="KQD82_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381824.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1903..3132
                     /locus_tag="KQD82_003377"
     CDS             1903..3132
                     /locus_tag="KQD82_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG4381825.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3140..3814
                     /locus_tag="KQD82_003378"
     CDS             3140..3814
                     /locus_tag="KQD82_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381826.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3817..5049
                     /locus_tag="KQD82_003379"
     CDS             3817..5049
                     /locus_tag="KQD82_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381827.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5335..6600)
                     /locus_tag="KQD82_003380"
     CDS             complement(5335..6600)
                     /locus_tag="KQD82_003380"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG4381828.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASKLGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6653..7801)
                     /locus_tag="KQD82_003381"
     CDS             complement(6653..7801)
                     /locus_tag="KQD82_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG4381829.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVSLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7901..>8015)
                     /locus_tag="KQD82_003382"
     CDS             complement(7901..>8015)
                     /locus_tag="KQD82_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG4381830.1"
                     /translation="AKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRHFGK"
BASE COUNT         2883 a         1176 c         1120 g         2836 t
ORIGIN      
        1 ttattttagg taataaggta gaaatatttt aatttatcaa gtagaatata gttatgttaa
       61 tataactatt ttgaattagg aggtaagaat gtttaaaata atggtagttg aagatgacgt
      121 ctcattgaaa aatatcatag caaagtgtct tactaaatgg ggacatgatg ttcatcaaat
      181 agaaaatctt gaaaatataa tagaagagtt caaaaactat aacccagaat tagttttatt
      241 ggacataaac cttccatttt atgatggttt tcattggtgt aatgaaataa gaaaaatttc
      301 aaaagttcct ataattttta tttcttcaag aaattctaat atggatgtaa taatgggtgt
      361 aaatttaggt gcagatgatt atatacaaaa acctttttct gtagatgtct tagtagctaa
      421 agttaatgcc cttttaagaa gaacttataa ttttgttgat aataactcaa atcagattat
      481 tcacaatggt gttactttag atttatcaac tgcaacaata aactatgaag ataatactat
      541 tgagctaaca aaaaatgaaa tcaaaattct ccatgagctt atgaaatata aggggcagat
      601 agtaagtagg aataaactca tgaaaaaatt atgggataat gattggtttg tagatgataa
      661 tacattaact gtcaatgtaa atagaatacg ctcaaaatta aatgaaattg gtttagaaga
      721 ttttatagaa acaaaaagag gattgggtta tataatcagt taaaacaatt ctaagtaggt
      781 gaatttatga gctttaaaga ttttctcaag gataaactag gttttttagt atacaatttt
      841 actttcttaa tttttacagt atcagttatt atattttctc ctgtagatgt ttttctgact
      901 gatactattt tatatatatt agttgtaaat gttgtgtttt tatttttata tctatttatt
      961 agctacatca agaagaacaa atttttagat aagataaaaa atgatacatt caaaattaat
     1021 atgaatgata tagaattagc tagatgtaaa aatgaagaaa aaatatactt gaacattatt
     1081 aaaaatcatc aatatcaatg caataacata ataaataata atatcgagaa atttgaagaa
     1141 aacaaagaga ttttaaatat gtgggtacat gatgtaaaga tacctatagc tataataaaa
     1201 ctcattcttg aacaaaatga aaatttaatt gatgaaaaaa tttcagatga catagatagt
     1261 gaaatattta gaattgaaaa ctccgttgaa aaaattttat atttatcaag actcgaagat
     1321 tttcataagg attttttaat tcaagaggta aatattgaaa aaatcattag aaatattata
     1381 aaaaaatact caaaatattt tatttcaaaa aaaattattt tagacttaaa taatttgaat
     1441 tttaaggttt tatctgatga aaaatggcta tacttcatat tcgagcaatt attgagtaat
     1501 tccctaaaat actcctcatt aggagataat atatctatct attgcaaaag aaccaaaaat
     1561 tatttacaat taagagtgga agatactggt tgtggtataa aaaaggaaga tttaaatcga
     1621 atattcaata agggatttac aggtaataat ggaagaaaca attctaaatc tacaggtctt
     1681 gggctttatt tagtaaaaga actttgtgat aagcttggtc ataaaattga tgtagattca
     1741 gagtacaacc aatatacaaa atttactatt acattcaaat gtgatttata gtatcttatt
     1801 attgatagtt tattttcatt aacttatatt caacttctta tagtaattct atatgtacat
     1861 cccagtatat aattaagtag tttttataaa aaggaggaag acatgtcttt tctaaaaaaa
     1921 tttaaaaata aaagatttag aaataacagt tttaaaagta acaactttag aaatagcagt
     1981 tttggaagta gtggcttcaa gtctaaaaat aaaggtttta aaaacaatcc ctttaaaaat
     2041 aagagattca aaatatatgc aggaatagca gtaatcatac ttttaattct aggagttctt
     2101 gcttatgttg attcgaaaaa cactgaatta caagctaatg aaaattttat cgaaacatat
     2161 actattcctg aaaatgaaaa aatatttata aatgggatgg ttgttcctaa gcaaactaag
     2221 gacttcaata tatctggaga ttatgagtta agtgatgtta atgttacaaa tggtcaaaaa
     2281 gtaaatcaag gagaccttct ttttactgct aaaaatccaa ctataatagc agaaatagat
     2341 agtttaaaat cacaacttag tcaatacaaa aaacaaaaaa tatcactttc tgatattgct
     2401 gaaaataaag atgctattgc ttctataaat gcacagataa ctgctttaaa ctctcagata
     2461 gcttctctgg ataaaaaagc ttatgacaga gttactgctc cttttgatgg cacagtatac
     2521 ttgaatgacc aaactggaaa tccagaccaa ccagtttcat ttatgactat tcaaggtcta
     2581 gagttttata tgaaaggaca agcaagtgaa caagatttac ctaaaatgaa gattgaccaa
     2641 atggtcaata tactcgtttt ttcaactgac caaaagctta ctggtagaat aagttttata
     2701 tctgataaac catctactcc aaatactgaa atgggagctc agcagaatac actatcttat
     2761 tatgatataa acatagcatt tgataatcaa gaaggattag taaatggatt ccatcttcaa
     2821 gcatcacttg aagtagctaa ttcaagcttt aaaataccag catcttgtgt acttaaagac
     2881 aacaaacact catatgtatt taaggattta gatggtatat tgaaaaagca aattgtagat
     2941 gtagcaagtc aaaatgatga ttttgctgta gttagaggcg gtcttgaaca aggcgatata
     3001 ataataaaac accctactaa agaaatgaaa gaaggagacc cagttcaagg cgatggcgta
     3061 actgctggtt ctaataatgg agatactact ccaaacaaaa aagtagaaga agttactatg
     3121 gatgtgaatt aatttacata tgttaattaa attagaaaat attcaaaaat attacaaagt
     3181 agggaaagat gaattacatg ttttaaaatc cttaaattta gaaattgaat ctggagaatt
     3241 tgtcatgata atgggtaagt ctggtagtgg taaaactact ttacttaata tcttaggttt
     3301 tctagatgta tttgatgaag gaagatatat atttgatgga acagatgtaa ctaatttgag
     3361 tgaaaatgaa cgttctgttt ttagaaatat taatattgga tttgttttcc aacaatttaa
     3421 tttaatagaa actctaaatg tatatcaaaa tgtagagttg cctctaattt acaataaagc
     3481 actaaaaaaa tctaatagag aagaaattgt taaagataaa ttaagttctg ttgggctttt
     3541 ggacaagctt aagcagaaac cacttcaatt atctggtggt caacaacagc gtgtagccat
     3601 tgcacgttgc ctagcaaatg accctcagat aatctttgct gacgaaccga ctggggcatt
     3661 agatagtgaa actagcagag aaattatgga gcttttaaca aggctcaaca aacaaggtaa
     3721 gactataata atggttacac atgatcaaga tttaactaag tatgcaacta aggttatacg
     3781 acttaaagat ggtgtgttta cttcggaggt gtaatcatga gtttattaaa aaattctttg
     3841 gccaatttaa aaggtcacaa gcttcgtgta tttgtagctt tactctggat tataatagga
     3901 ataacttcag taatacttgt tagttctata ggtaatggat ttcaaaaaga aattaagaag
     3961 tctgtaaaca atgtaaaccc taataaaact actatttcgt ttgaatcagc cgataacaca
     4021 ggtcttactg atgatatgag tattttttta aaacccttta atgcaaaaga tttagaagaa
     4081 ttatcatttg ttgaaggagt agaaagaata gctccttcaa gagatgggtt taaccttgat
     4141 tcagtttact cttcacaagc atcttttgat aaaaaaacta cgtatgttga tgttggacca
     4201 gtaaaaaaag attctaaaat aaatttaatt tgtggtagag atttttcttt agatgatgaa
     4261 aaaagaaaag taattttact tacattacaa agtacaagtg aaatttttga aaatccagaa
     4321 gatgccttag gtcatggtat caacataaat ggaactatat ttgaaataat aggggtatta
     4381 gatgattctc agcaaaacca agctggagga ttctttggag gttaccaaga tatgcaattt
     4441 actacttccc tagtacctaa aaaggctttt gacactttaa tgagtcaaaa ttcttattca
     4501 aatgaaattt accagttaga cttagtttct tctaaaggct ataatgtaaa tgaagttgca
     4561 aacaatgtta tagctaaatt atatgagatg caccctggta tcaatggttc ttatactaca
     4621 ccagacccaa ctgaacagac agcttatctt gaaagcatta attccaatgt aaacaaatat
     4681 gtatctataa taactgtagt tgcaatgttt gttggtggta ttggtgtaat gaatataatg
     4741 tatgtatctg ttatggaaag acaaagagaa attggtataa gacgtgctat tggagcaaaa
     4801 cctaggtcta tattatttca atttttagtt gaagctgttt ttataacagt atgtggtgga
     4861 attttaggaa ctatagttgg atttactgct actaactatg tttctaaata tataggtttt
     4921 gaagctatac ctagcttaaa tagcttattt tatgcaattg tagctactat attgactgga
     4981 gttgtatttg gtctgattcc agcctttaaa gcctctaaac tagaccctat aaaggcaatt
     5041 tataagtaga aattgtataa agattttcga tttttagatt agtttatata aaacatactt
     5101 aaatatataa cgcactatta tatatgatag attaggaact taatctttcc aaatataata
     5161 gtgcgttctt tcataaaaag gattctcttc cttactatat ctaaattacc aaaatgttat
     5221 gttctaattg ttcaaactaa tacttccaat tttgattaat caatctcatt ctgctaatat
     5281 acaatctttt actttcaatc attgtacact ccgccttaaa aaaataatct taaattaggc
     5341 gttatataca atctccccat tacatattgt ataattaacc tttccatata aagatttacc
     5401 tttaaacggt gaattatcag ctttagaaac aaagctttca actgtccatt cttcttctgg
     5461 attgaatata acaatgtctg ctacagcccc ttcttctata aatccaatat ttaaattgta
     5521 caactttgca ggatttatag acattttttc tattaactgc atcatactaa gatgattttt
     5581 atgcaccaaa tttgttattc caagagctaa agaagtttca agcccaataa ttccacttgg
     5641 agcttttata aactctctat ctttttcttc tttactatga ggtgcatggt ctgttgctat
     5701 tatttcaata gtaccatcat ttaatccctc tataatctta tctctgtccc atttacttct
     5761 tagaggtgga ttcatttttg catttgttcc aaactccaat acatcttcct cagtcaatgt
     5821 aaagtgatgt ggtgaagctt ctgcaactac atttgcacct aagtattttg caaatctaac
     5881 catatcaaca gatacacctg aacttatatg ttgtatatca actttagcac cagtctttag
     5941 tgccaacata caatctctgg ctaccataac atcttctgct acacttgatg caccttttaa
     6001 tcctagtttt gaggctacct ttcctgcatt tacacctgga tttccaacta aagaagggtc
     6061 ttcttcatga aaactaagtg gtacatccaa ctcttttgcc ttaatcattg cctccataat
     6121 tagattgcta tccattaaag gtaatccatc atcagtaaac cctggtacac cagctttttt
     6181 aagagcttcc atatcaacta actctttacc ttcaaaacct ttagttatag ctgctgcttg
     6241 taaaacattt atacatgcat tttttgactt ttctttaaca taattaaaag tatcttcatt
     6301 atctacaata ggattagtat ttgccataca tataacagtt gtaaatccac ctctagcagc
     6361 agatttagct ccactttcta tatcttcttt atatgtaaac ccagggtctc taaagtgtac
     6421 atgtacatcc actaaacctg gagcaactac attaccacta gcatctatta ttttttcata
     6481 ttcatcactt tcatcaaact tgcctatttt ataaatttta ttttctttta taattaagtc
     6541 aatattctca tctctttgac ttttagggtc tattactcta ccatttttaa ttaaaatcat
     6601 attatttctc ctttatattt ttaatttata tagactcata taatattata cattatttta
     6661 catacttaaa tacaaaatta cgtttgacat caatagcctt tttaggacaa agttcatggc
     6721 aacagtaaca tcttatacat ttacttaagt ctataatagc acccttagtc cctatctcta
     6781 tagtggatgc aggacaagct tccttgcatt ttccacattt tatgcaatct tgaaatctta
     6841 ccacaggctt tggtgttata agctttgaaa taggttcatt taaaaatttt ggtagcttta
     6901 cggaaaacaa tcttaaatct ttacttacat ttggaatctt gtaatttttt ataattaaat
     6961 cttttatatc ttcacctaaa atttcaatat ctgaaaaatc ttcttgtata tatcctctct
     7021 taatagcacc tctaattgta gcaactctat ttggaagtaa acttataatc ttacatgcaa
     7081 caacatctat agcatatgga ttttgtgatg ctaatgaaac tcctatttct cgagtagtac
     7141 cagctgaagg tccatcacct tccataccta ctataccatc cataattgtc aatgttggtt
     7201 ttatataatc acaaatatct aaaagtacat tttcgcaaaa caactcttct gtaggaaatc
     7261 tataatgtat ctctgctttt ttcaatccag ctatgcatcc atataagttt tttgtacccc
     7321 cagtaaaagt tgccattata tgagtcttta atttacaaag atttattata taatctgaat
     7381 ctactattgg tttaataata tccatatatt ttagaacatg tgctttttca ctatgtatct
     7441 taacatctga aacatcataa tttaacttaa tgcccagttc atttgctact tcttcaattc
     7501 cagatgcctt ataaatgctc ttaagagaac tttgattata tggaccacca gggctatctg
     7561 ctattgtaat atcacaattt aactctaaaa gtttttcaca tacaactttt acaaccatag
     7621 gatgtgtagt tgtagcttct tcaggctttt ttttcatcaa taaatttggt tttataagta
     7681 ctttgcttcc ttttttaaca aacttatcta taccaccaat atcctctaaa tttttttcaa
     7741 tagataactt tacttcttca taattgtaag ttttacaact cctaacagat accgacttca
     7801 tattatctcc atccttctat cacaacagtt tatttatata atttaaaaaa ggagaaacca
     7861 aagtttctcc ttttaatcta tgttttaatt gttttaatta ttattttcca aaatgtctgt
     7921 ttaataatcc taagaatgct ttaccatgtc tagcttcgtc tttacacatt tcatgtactg
     7981 tatcatgtat agcatctaat cctaattctt tagct
//
LOCUS       DAENCV010000157         8010 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAENCV010000157 DAENCV010000000
VERSION     DAENCV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.160"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..486
                     /locus_tag="KQD82_003383"
     CDS             <1..486
                     /locus_tag="KQD82_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4381831.1"
                     /translation="LGTCPSMTVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVS
                     LDLKKYLDVQVKFLSGGQRQSLSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTDK
                     IVREKNITTLMVTHNLKHALHYGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYA
                     V"
     gene            617..1639
                     /locus_tag="KQD82_003384"
     CDS             617..1639
                     /locus_tag="KQD82_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4381832.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1803..2360)
                     /locus_tag="KQD82_003385"
     CDS             complement(1803..2360)
                     /locus_tag="KQD82_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG4381833.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2366..3442)
                     /locus_tag="KQD82_003386"
     CDS             complement(2366..3442)
                     /locus_tag="KQD82_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG4381834.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLNFPQEYLFCFRYNYNKKE"
     gene            complement(3443..4126)
                     /gene="modB"
                     /locus_tag="KQD82_003387"
     CDS             complement(3443..4126)
                     /gene="modB"
                     /locus_tag="KQD82_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG4381835.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4275..5087)
                     /gene="modA"
                     /locus_tag="KQD82_003388"
     CDS             complement(4275..5087)
                     /gene="modA"
                     /locus_tag="KQD82_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4381836.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5280..6209
                     /locus_tag="KQD82_003389"
     CDS             5280..6209
                     /locus_tag="KQD82_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381837.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6370..6819)
                     /locus_tag="KQD82_003390"
     CDS             complement(6370..6819)
                     /locus_tag="KQD82_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381838.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7022..8002)
                     /locus_tag="KQD82_003391"
     CDS             complement(7022..8002)
                     /locus_tag="KQD82_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381839.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2767 a         1325 c          959 g         2959 t
ORIGIN      
        1 cttggaactt gcccttctat gactgttaga gaaaacttgt ctttagctct taacaaagga
       61 aaactattaa atctaagaaa atgtcttcgt tacaaaagag atttcttaga aaatttattg
      121 gaaggtgtat ctcttgattt aaaaaaatac cttgatgttc aagtaaaatt tttatcagga
      181 ggtcaaagac aatcattatc actaattatg tcgtgcttaa caaatccatc agttttatta
      241 cttgatgaac atacagcagc acttgaccca aaaacatcaa atgaagtaat agagttaaca
      301 gataaaatcg taagagaaaa aaatataact acacttatgg ttactcacaa cttaaaacac
      361 gctcttcatt atggtgatag attgataatg cttcataagg gtgaagttgt attagatgta
      421 aaaggtaaag aaaaagaaca attaacagtt gaagaaatct tagaaaaatt tgaatatgca
      481 gtttaaaata atatatctat tgtattaaac tcggctttaa aataaagtta aaattacaat
      541 aattatggtt actttgtaat aattcatatg aatatatgaa catatataat aataaatttt
      601 tgagggggat ataaaaatga ttaataaaaa aagattggca agtttgattt tagctggtgc
      661 tcttagtata tctatgctta cagggtgttc acaaggagga gactctggta attcaaaaca
      721 agaaagtaat tctaaaggta aagaagttaa aaaaattggt ataactcaac tggttgaaca
      781 tccagcatta gatgccacta gaacaggttt tgtaaaagca ttagaaaaga acggatttaa
      841 agatggagaa aatattgata tagacttcca aaatgctcaa aatgacatgc ctactacaca
      901 aagtattgcg agtaaatttg catctgataa gaaggactta atatttgcta tatctactcc
      961 atcagctcag gcagcattta atgctactaa agatatacct atacttatta ctgctgtgag
     1021 tgaccctgtt gctgctggat tagttaaaac tcttgaaaag ccaggcacaa atgtatctgg
     1081 aacatcagat tttgtttctg tagacaaagg tttagaactt cttaaaatat ttgctcctaa
     1141 ggcaaaaact ataggagtta tgtacaatac tagtgaagta aattccaaag tccaagttga
     1201 tgctttaaaa gaatatgcat ctaaaaatgg atttaaagtt gttgaaaaag gtataactac
     1261 ttcaaatgaa gttaaccaag gtatttctag tttagttggc aaaatagacg tattatatgt
     1321 tcctacagat aacttagtgg cttcttctat gcctatagtc tctaaaatag ctactgagaa
     1381 taaaatacct gttatagcag ctgaatctgg gccagttgaa aaaggtgctc ttgcttgtca
     1441 aggtatcaac tacgaaaaac taggttacaa aacaggtgaa atggcagtta aaattttaaa
     1501 tggtgaatca gtatctgata tgcctgtggc tacatcagat gatacagata tcattgttaa
     1561 tgaagatata ttaaaagctt taggtatgga aaaaccttct aatgaaaata tttcttatgt
     1621 aaagactaaa caagaataga aagatatttt ctatatacga ttaatatgcc tgtttctata
     1681 aattaattta tagaaacagg catatatttt tattttaatg tattatctat ttaaagatta
     1741 tatttaaatg ctcttaattt gtttgtataa attttacttg tataaacttt atacaatcat
     1801 atttattctt ctattttctt tattactcta catggatttc caacagctac agtatttggt
     1861 ggtatatctt ttgtgactac acttcctgca cctattacta cattatcacc tatagtaatt
     1921 cctggtgtta tgattactcc cccacctatc catacattat cacctatttt tacaggagaa
     1981 ccatactcta tgcctgaatt tctaagttgt gcatcaattg gatgataggc tgtatagatt
     2041 tgaacgtttg gagctaacat tacattgtca cctatttcaa ttttacatac atctaaaaaa
     2101 atacaatcat aatttgcaaa gaaattttcg cctacatgta tgttatatcc ataatcacac
     2161 ctaatgtttt gttctacatt tatttgcttg ccaactgaac caaatagttg tcttaaaata
     2221 tcttctcttt tttcatattc atcctctaaa gtattattaa acaatctagt taatttttta
     2281 caatactctc tttcttttac taacaattca tcatttgcat agtatccttt cccagacaac
     2341 attttttctt tttcagtcat atagtttact cctttttgtt ataattgtat ctaaagcaaa
     2401 ataaatattc ctgtggaaaa tttaaatata ttctgtcacc tttcttaaat ctcattttac
     2461 ttttttccaa ttctatttga ataggtacac attcgttaga taaatcagtt tttctaacat
     2521 atatagtata tgtaaaagga ttttctacaa tgttaatcaa ttcgaaacat aacttttcat
     2581 ttaaatcttc attagaatcc aagaccttaa tatgatgctc cctgattccc acatattcta
     2641 tattatcacc tatttctcta tttaatgtca actcgcatcc ccagtctgta gcataaatag
     2701 tatttttatc cagtctattt aatttggata tatttttaca ccctgttatc ttagcttcta
     2761 taaggtactt tggagattca aagagctctt ttttaggtct ctttggaaga ccttctcctt
     2821 tgttgtacac tataatatca tcacaaactc ggtaagcttc ttcaatatca tgagtaacaa
     2881 atagtatatc tcccttgtaa tcttttaaca tattcataag ttctttttcc atattacttc
     2941 ttaaatgatg gtccaatgct gagaaaggtt catctaaaag taatatatct ggagatgttg
     3001 ccaatgctct agctagtgct accctttgtt gttgaccacc agataattgc caagggtatc
     3061 tcccctcaaa cccatttaat ttaagtatat ctaagtacct tgctactatt tccttttttt
     3121 cactcttact tagttttaaa agcccaagtt ctatattttg acttacagtc atatgtggaa
     3181 acaaagcata gttttgaaat aaaaatccta cctttctatc ttgtgttgat aaatttatct
     3241 tcttttcaga atcaaataga actctatcat ttaatacaat tttacctctt gttggtttct
     3301 caagccctgc tatacatttt aatgtcatac tttttccaga accagattct cccaaaaacc
     3361 ccagagtacc tttttcttgt ttaatttcta cctttagttt aaaagaggat aaatcttttt
     3421 caatatccac gtataagctc aactatccac acctctttcc tataattttc tgctgaaatt
     3481 ccgaccaata atttagtaat aaaatcattg ctatagatat tgctacaata atcataaccc
     3541 acatcatagc tttattcata tctccgcctt caacagcaaa aaatatagct attggcatag
     3601 tttgagtttt tccaggtata tttccagcta tcataagtgt agcaccaaat tctccaagag
     3661 ctctggcaaa cgacagcacc aatccaccaa ttattccagg ccatgctaat ggaactgcaa
     3721 tcttaaaaaa tactttccat tcattaagac ctaatgttct agcagctgac aacatattat
     3781 tatcaatctg ttcaaaagct gaccttgaag ttctatacat cataggaaaa gacaccacta
     3841 ctgctgaaat tacagttgct gtccaactaa atattaaagt cctatcgaaa tttaataaaa
     3901 actttcctat aaatccattc ttcccacata gtaataataa aaagaaacct acaactgttg
     3961 gtggcaaaat tagtggaagt gtaaatagtc catctataag acccttccac ttacctctat
     4021 agttagccat aatatatgac acaaatattc ctattacaaa agttattatt gttgacaaaa
     4081 tagatgtctt aagtgatatc cataagggtg accaatctgt tcccatcttt ccctccttaa
     4141 ttttaaatgg aaatagtgaa actaataact ctattaattt cactatttcc attttattat
     4201 aatacacaat tattttcaat tttcatttat ttatatataa tttatactta attacacata
     4261 atttatttct aagtttattc tactttttta tatccaaatt cttcaaaaat cttttgtcct
     4321 gactcactta ataaaaattc ttcaaatttc ttagcagcat ctacgttttt actagcttta
     4381 attactgaaa ctggatatgt aataggcgaa tgagtttttt cgtctgtttt ttcaactact
     4441 tttatcttat cattattgac agtatcactg aagtaaacga atccaacatc agcatttcct
     4501 gattgcaccc atgctaaaac ttcttttaca tcttttgcaa atactagctt atcttttaac
     4561 ttatctttta aatttaaatt agttaacacc tcatctgcat acttaccagc tggaacactt
     4621 tcagcttcac caacagcaat tttcttcact ttatctgtag tcaaatcttt catacctgat
     4681 acactactgt cagcagaaga aataagaact aaatcatttt ttactaaatc tttcatagta
     4741 tcagaaacta ataacttctc ttcatccaaa gctttcattt gtttttgacc tgctgaaata
     4801 aataaatcac aaggtgctcc ttgttctatt tgttgttgta atgagcctga tgctccatag
     4861 ttaactgtaa gttttacatt tgaatcaact tttttatatt cttcttcaat ctttgccatt
     4921 gcttctttta aacttgcagc agccgatata tttaactcta ctgaatcact acttttagtt
     4981 gttttttctt gttttccatc atcttttttg tcttcattac tattgcaccc aactgtcccc
     5041 aaagttaaac atgcaactaa tcctaatatt cccaatattt ttttcataac aatctccctt
     5101 tttgtaaatt tgatacacat tttaacatgt tttattatgt tttatcatgt tttgttatat
     5161 atagtttaat tatatataca tgataataaa ttttcaagta atttatttac actactttta
     5221 aatattttgc tacattttcc tttttttaat gttaaaataa ccttatatgg aggtggatta
     5281 tggactcatt atcatctttg acacctgtcg aggtagctga attattaaag ataacaaaaa
     5341 atactgtata tgaattaatc aaaagaggtg aacttccttc ttataaagta gggaaaaaaa
     5401 taagagttga tactaaagat gttgaagaat atatcaacag ccaaaaaact ggtaaagtaa
     5461 aggatacaca tttcaacaat aaaaatatag atataaataa taatttcaaa attattattt
     5521 ctggtcaaga tgtaatatta gacattttag ctaggtctgt tgaaaaaaaa ttggatggaa
     5581 taagtacatt tcgctcttat ataggtagtt ataatgcttt gtatgagtta tataatggca
     5641 gagtttctat agcatcatcc catctatggg atttcgaaac ggatgaatat aatagcactt
     5701 ttgttaaaaa gctattgcca ggaataccat gtgtacttat aaatctagct tatagaatgc
     5761 aaggtttcta tgttgctaaa ggaaatccta aagagataac aacctgggag gatttaacaa
     5821 aaccaagtat aaccattgta aatagggaaa aaggttctgg aactagggtt ttactagatg
     5881 gtaaacttag acttctaaac tttaatggta aacatataaa aggttatgat aatgaagaat
     5941 tatcacatct tggagttgca agtactgttt ctcgtggaat agcagatgtt ggtttgggaa
     6001 atgaaaaagc tgctttacaa gtaaataata tcgactttat tcctcttcac aaagagcgct
     6061 atgatttagt tattaaaaaa gaagacttac aaaatccagt ttaccaaact ataattaaca
     6121 taataaactc tcctgagttt aaagctgaac ttcaaggtct tggcggatat gatttaactg
     6181 atactggaaa aactatagca aaagtttaag attaaaatcg caatctattt tataagattt
     6241 ataatttttc acaaaattta taacttaaac acgaaataaa ttgcgatttt agtgtctcaa
     6301 aaaatttatg tctttcattt tatcatccaa tacaaaatat aatataatta attgtgcaat
     6361 catattatat taatatatat ttacataata gtatttttct ttaatgtaga ttaacatatg
     6421 atatgtttta aacatcaatc cccttgcttt cttatatata tgtggcaaca catatgagtt
     6481 taataaatta tatacaatgt tgctttcttc ttgtattttt tcaaccaaca agttatttat
     6541 aaaattacct ttctcatttt caataatctc actccaattt atcttaactt ttaatatata
     6601 tcttcccact aggtatacac actctacaat aaataataaa aatacaatgc agtcagtacc
     6661 tgttgtctta tataaattac ccgatactaa tggctttgcg aagaaattat aaaaattcaa
     6721 agtatcaccc gtataatcaa aaagtattaa tagcactcct agaactctca atactttaat
     6781 attcaacatg tttttatctt tattattttt gtttttcata tatactacct ctcttttctt
     6841 tagatttgat tgtaatctaa tactaactct tttatttgag gtataaatga atttaattgg
     6901 tagttatttt catttttact agaaattaat tcacattact aaacaaaact tatatttaaa
     6961 cataaaaata gcctatctat cttttaataa atacaaaaat aaataagcta ctaatattca
     7021 attaactttt atcacttaaa acattactat ctataaattt aaatatttta ttgaagtata
     7081 ctgtactatc atgttcaaat ccatcaacat gaccaatacc ttctataagc cataggtctt
     7141 ttttatcatg tttaactaaa tcatacattt tcttgctatt ttgataatta caaacagtat
     7201 cttttgttcc atgtataaac agtatgggta cattagattt ctcaactgac tcatatggtt
     7261 ttacatcact gtaccaaaat ccagatttaa acttattata taaatctcca caagtcacca
     7321 cataattgat taatatatct ggtattcgtt tttctagtac tcccattcta attgcatctt
     7381 tcatttcaga ataaggagaa tccagtatat aaaaatcgac cttatcatct tttgaattta
     7441 tctctgaatg catagcagct gtccctgccc ccatggaaaa tccatgcact cctaatcttc
     7501 cttcaggaaa tttatttttt acaaatttaa ctagactatc taaatcatac ctttcataaa
     7561 gaccaaatgt gtaattatct ccaccactat taccagtatt tctttgatta taaactaata
     7621 cattataacc tttatctaaa tatctatatg ctacctttaa catttcatag taactacttc
     7681 caattccatg aacaagtatc atagtatctc ttgtctttat atttgaagtt ataaaaagac
     7741 tttcaagctt gtaattatta gaactttgta tcattaattc agatgtcttg tagtttttta
     7801 atttctccag tggtttatct tttctctcag aaaatatttt tactatatct tcgtttttta
     7861 ttaactgctc taaaccaact gaactattat acactaattt gccaaatata taagatacaa
     7921 ttaataggat taatagaaac acaattgata taattataaa tttatttctt aaactaatac
     7981 aatttttaaa actatttttc atatttattt
//
LOCUS       DAENCV010000158         7891 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAENCV010000158 DAENCV010000000
VERSION     DAENCV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.161"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            70..2415
                     /gene="secA"
                     /locus_tag="KQD82_003392"
     CDS             70..2415
                     /gene="secA"
                     /locus_tag="KQD82_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG4381840.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            2830..4701
                     /locus_tag="KQD82_003393"
     CDS             2830..4701
                     /locus_tag="KQD82_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG4381841.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            4808..5515
                     /locus_tag="KQD82_003394"
     CDS             4808..5515
                     /locus_tag="KQD82_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG4381842.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSENEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            5548..7383
                     /locus_tag="KQD82_003395"
     CDS             5548..7383
                     /locus_tag="KQD82_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG4381843.1"
                     /translation="MKISKKIVSLLTITFLTIMLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKDVPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(7431..7826)
                     /locus_tag="KQD82_003396"
     CDS             complement(7431..7826)
                     /locus_tag="KQD82_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG4381844.1"
                     /translation="MILKKYKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
BASE COUNT         3343 a          852 c         1427 g         2269 t
ORIGIN      
        1 taattgtcta ctattctata attttaaggt ataataataa ttgtatgata aaaaaggagg
       61 tcaaaaaata tgtcagtttt agatgcaatc ttagataaag cggatgaaca agaaattaaa
      121 aaattaaatt taatagtaga taaaatagaa tctttagaaa aagatatgga aatcaaagag
      181 aatgaacaat taaaagaaat gacaaatact tttagactta gattagacaa aggtgaaagt
      241 ttggatgata tacttccaga agcatttgct gttgttagag aggtctcaaa aagagtttta
      301 ggaatgcgtc aatataaagt tcagttaatt ggaggtatag taattcatca aggaaagata
      361 gctgagatga agactggtga ggggaaaact ttagttgagg tagctccagt atatctaaat
      421 gctttaacag gaaaaggtgt acatgtaatt acagtaaatg attatttggc agaacgtgat
      481 aaagaactta tgcgtccagt atatgaattt ttaggattga ctgtaggtgt aattttatct
      541 aaccaagacc caagtataag aaaagaacaa tataaatgtg atataactta tggaacaaat
      601 agtgaatttg gatttgacta tttaagagat aatatggtgc cagatttgtc tcataaagta
      661 caaagggaac taaactttgc catagtagat gagatagact caatattaat agatgaagct
      721 agaactccac ttattattgc aggagatggt gatgaagatt taaaacttta tgagttagca
      781 aataactttg taaaaactgt taaagaagaa gactttgaga tggatagaaa agataaaact
      841 atagcattaa cagcaagtgg tataagcaaa gctgagtcat tttttggtat aacaaacctt
      901 actgatataa agaatataga attatatcat catataaatc aagctttaag aggtcataag
      961 cttatggaaa aagatgttga ctatgttatc tcaaatggag aagtaatgat agttgacgaa
     1021 tttacaggaa gagtaatgga tggtagaaga tatacagatg gacttcacca agctatagaa
     1081 gccaaagaag gtgttgagat aaagaatgaa tctaaaacta tggctactgt gacttatcaa
     1141 aatttcttca gactatatga aaaactttct ggtatgactg gtactgcaaa gacagaagaa
     1201 ggggaatttg agtcaatcta taaattaaat gttgtccaaa taccaactaa tagaccagtt
     1261 attagagctg atttgcatga taaggtattt aaaacagaag aagaaaagta tagtgctgtt
     1321 gtagaagaaa taataaggat acataagact agacagccaa tactcgtggg aacagtttct
     1381 gttgaaaaat ctgagaagtt atctaaaatg cttaagaagc aaggtattaa gcatcaagtc
     1441 ttaaatgcta aacaacatga taaagaggca gaggtaattt ctaaagctgg taaattagat
     1501 gctataacga ttgctacaaa tatggcaggt agaggtacgg atatttctct aggtgcagga
     1561 gataaagaag aagaagaaaa agttaaaaat ttaggtggtc tttatgttat aggaacagaa
     1621 agacacgaat caagaagaat tgacaatcag cttagagggc gttctggtcg tcaaggagac
     1681 ccaggtacat caagattctt tgtaagtctt gaagatgatg taataaagct ttatggtgga
     1741 aaaactatag agaaacttat gaagagaaca agttcaaagg aaaatacggc tattgaaagt
     1801 aaagcactta caagggctat agaaagagca caaaaaggtg tagaaggtaa aaattttgaa
     1861 ataagaaaaa atgttcttaa atatgatgat actattaatg agcaaagaaa ggttatatat
     1921 aatgaaagaa ataaagtgtt aaatgatgaa gatataaaag aagatattca aaaaatggtt
     1981 aaagacatca tacaagaagc aggagaaact tatttaattg gaagaaaaag agattattat
     2041 ggatatttta aacatttata tagtacattt atgcctgcag atacattact aatacccggt
     2101 gtagataaaa aaagtgtcca agagataatt gatagcacat atgaaatttc aaaaagggtt
     2161 tatgacttga aaaagatgat gattggtatt gataaagttg cggagttaga aaaaacagta
     2221 cttttaaaag tggttgatca atactggata gatcatatag atgctatgga acagttaaga
     2281 cagtatatag gtcttaaatc ttatgctcaa aaagatccgt ttaaagaata tgctttagaa
     2341 ggatatgata tgtttgaagc tttaaataaa aatataaggg aagcaacagt acaatactta
     2401 tataaattta actaaaaaag ttcatgtaat ttttattaaa tgaaaaataa aatattttga
     2461 ttctttatat tgtaattaca attaaataat taaggaatac aaatgaaaaa gaagctgaaa
     2521 atcagcttct ttttctgtgt gagaaatacg gaaaaaagag atgcttgaaa tttctttctt
     2581 ccatttaata ttatactata aagaagatga aaagtctatt agttataaac tggattatta
     2641 aaaaaaatta caattttgtt acttttttgt atacataaat attaaaaaaa taaaatataa
     2701 gaaaaagttt atatcttttg gttaattatt acaataagtc tcatttattg aaataatatc
     2761 aaatatatat tataattggt aaataaggaa aaaataataa aaatttgaat tttttagggg
     2821 gaaaatacca tgaataaaaa aaatctttct gtaattatgg ctgctgcaat gataagtaca
     2881 tcagtagctc cagtttttgc tgcagaaact acacaggtaa aaaaagaaac aataactaag
     2941 aaagaggcta cagaattagt ttcgaaagtt agaggtttaa tgtctcaaaa gtatacagga
     3001 ggttctcaag ttggacaacc aatatatgaa ataaaagttg gtgagacttt atcaaaatta
     3061 aaaataataa ctaatataga tgaattagaa aaattagtaa atgctttggg agaaaataaa
     3121 gaacttattg taactataac agataaaggg catataacaa atagtgcaaa tgaagtagtt
     3181 gcagaagcaa ctgaaaaata tgaaaattca gcagaccttt ccgctgaagc taattctata
     3241 acagaaaaag ctaaaactga aaccaatgga atttataaag ttgcagatgt aaaagcttca
     3301 tatgatagtg ctaaagataa gttagttata actttaagag ataaaacaga aacagtaact
     3361 tctaaaacta tatatgtagg tattggtgat gaaaaagttg atttaacagc aaatccagtt
     3421 gattcaactg gaacaaattt agacccttct gcagaaggat ttagagtaaa taaaatcgat
     3481 aaactaggtg tagcaggagc taaaaatatt gatgatgtcc aattggccga aataactata
     3541 aaaaatagtg acctaaatac agtttcacca caagatttat atgatggata tagattgact
     3601 gttaaaggta atatggtagc aaatggtaca tcaaagtcaa ttagtgatat ttcagcaaaa
     3661 gattcagaaa caggaaaata taaatttact attaagtata ctgatgcatc tggaaaagca
     3721 acagagctta ctgtagaaag tactaatgaa aaagatttaa aagatgccaa agctgcatta
     3781 gaaggtaatt caaaggttaa attgatagct ggagatgata gatatgcaac tgcagtggct
     3841 atagcaaaac aaacaaaata tactgacaat atagttatag ttaattcaaa taaactagtt
     3901 gatggattag cagctacacc acttgctcaa tctaaaaaag cacctatatt attagcatcc
     3961 gataatgaaa taccaaaagt aactttagat tatataaaag atataattaa gaaaagccca
     4021 tcagctaaaa tatatatagt aggtggagaa tcagcagtat caaatacagc taaaaagcaa
     4081 ttagaatcag taactaagaa tgttgaaaga ctagctggag atgatagaca tatgacttct
     4141 gtagcagtag caaaagctat ggggtctttt aaagatgcat ttgtagtagg tgcgaaaggg
     4201 gaggctgatg ctatgagtat agctgccaaa gctgctgaac ttaaggctcc tataatagta
     4261 aatggctgga atgatctttc agcagacgct atcaaattga tggatggaaa agagattggt
     4321 atagttggtg gttctaacaa tgtatctagt caaattgaaa atcaacttgc tgatattgat
     4381 aaagatagaa aagttcaaag agttgaagga gaaacaagac atgataccaa tgctaaggtt
     4441 atagaaacat attatggcaa gctagataaa ctatatatag caaaagatgg atatggaaat
     4501 aatggtatgc tagtagatgc attagcagca ggacctctag cagcaggtaa aggtccaata
     4561 cttctagcta aaactgatat aacagactca caaaagaatg cacttagtaa aaaattaaat
     4621 cttggtgcag aagtaactca aataggtaat ggagttgaat tgacagtaat acaaaagata
     4681 gctaaaatac taggttggta ataacttata ctgtggagaa gattccttaa ttaatacagg
     4741 aatctttttc tataattatg ctatttattg taaattataa cattttataa agtaggagga
     4801 tgtagtagtg gtagatgcaa ctttattttt tacaccacat caagatgatg aaaccttaag
     4861 tatgggaagt gctataattg aacatgttga aaaaagtgat acacatgtca tattatgtac
     4921 agatggtagt aaatctataa taagaaaagt tttggatgat ggagggtcat gttcttatca
     4981 tattaaagat gttcataaat actctttatc agaaaatgaa ttttcaaaag atagagatga
     5041 agaatttaag gatagctgtg aagcaatggg tgtaaaagaa agtaatatac atatagaaga
     5101 taatagagca catgatggtg agttaagtaa agaaaaggca agggaaataa tacttaagta
     5161 cttagaagaa tatccagatg caaaagtaaa aactgttact ccgtttaaag ctagtggaat
     5221 acatgaagac catagggcat taggagaagc tgcactagag ttatataaag aaggcaaaat
     5281 aaaagattta agattttatg tagaaccata tgactataag gattttaaaa aagttaatcc
     5341 aaatgtagag gtatggaagg tattacctag tcaagaagag aaattattaa gtgcaatgaa
     5401 tgcatataaa aaatggaatc ctgaaagtgg acattatgct ataggatacc attcagtaaa
     5461 atctcatttt gatgagctag ctacaaataa aatacagtat gtacatgctc cataaaatat
     5521 tatgaaagaa ttaggaggta agaattaatg aaaatatcaa aaaagatagt gtccttgtta
     5581 actataacat ttttaactat tatgttatat ggaaatacat ctaatgcatc tacaaaagat
     5641 acattaacag gttctggaag atgggaaaca gcaataaaaa taagtcaagc tggatggaca
     5701 aagtctgaaa gtgctgtatt agtaaatgat aattcaatag cagatgcttt atcagctact
     5761 ccatttgcaa aggcaaaaga tgtaccaata ttattaactc aaagtaataa attggatagt
     5821 agaacaaaag cagaattaaa aagacttggt gtgaaaaatg tatatttaat aggtggttca
     5881 attgcattaa gttcagagat tgaaaagcag ttaaatgcag aaaatataag ttttgaaaga
     5941 atatctggaa atagtagata tgatacttct ttaaaattag ctgaaaagct agatagggaa
     6001 aaatctatat ctaaaatagt agtagtaaat ggagaaaagg gtcttgctga tgcagtgagt
     6061 gttggagcta tagctgctca agaaaacatg ccaataatac tttctgattc agagaatgga
     6121 actgaagtag ctgataattt tatagatagt aaagatatag aaaaatcgta tgtaataggt
     6181 ggtacatatt ctatttctaa ttctgtagaa agaagtttac caaatgcaac aagaatagca
     6241 ggtagtagta gaagtgagac aaatgcaaaa attatagaag aattttacaa agatactgat
     6301 ataaaaaata tttatgtaac aaaggatggt acaaggagta agcatgattt aatagattct
     6361 ttagctgtgg gtgtattagc ttctaaaaat ggttctccaa tacttttagc aggaaataag
     6421 ctagatagtt ctcaaaaaga tgtgttaaac actaaaatta tagataaagt tattcaaatt
     6481 ggcggattgg gtaatgaaaa tgttgtagaa gatgtaatag atatgcaaga agaaactaag
     6541 tatactgttg aaactattga tgaacttaat gttgctataa aaaaatcaga tgcaaatgac
     6601 atcataagat ttaagccaga aaaagataaa aatataacta attcttttaa aatagaaaca
     6661 aataaagcta taactattga atttgatggt atatataaac aatcaataac tatagatatg
     6721 ccaaatggag atataaaaaa ctttggggac atagataatg atctagtaat aaataatata
     6781 aaaaatgaga ctttagttaa cgaaggaagt atacaaggga tagatgttta tgatgagaat
     6841 ggatgtaaaa tagagaatga ggattctgga gaaatatggc ttataacgat acttaaaaat
     6901 tcaaaagatg tatatatttt gaatcgtgga gaaataacta aaatatctaa taattcttct
     6961 agtgtaacaa taaaaaattc aggtaatatt aatactataa gtggaagtga agaaccatca
     7021 ataagtggta aaaaacctat aactaatgat acagataaag atgaagataa aaaggcacgt
     7081 ggtttatatc ctaaagtaga atcttgtata cctgctttaa aagattatgt aagaataact
     7141 atatcaacaa aaccagagag ttcaaagtat aaaatttatt atagagtagt tgatgaaaaa
     7201 ccatctgcga aatatgttgg agaaaaaatt agtactaaag gttggagtat ggttacaata
     7261 tcaaatcaat cttttattga aagagccaaa gatggatcat atatagaagc agtagaaata
     7321 gatacctcaa ataatgaagt ttcacgatgg ggaaaatcta gcattacaga tgatggaatt
     7381 tagacattaa acttattcat tttgcagatt actatttagt aatctgctat ttatttttta
     7441 aaaattatca tcttgctaaa caagtaattt aaaactataa caactatatt agtaaatatt
     7501 tttacaataa catcatttac aaaaagcaaa tcaatcatta catacataat taacatttca
     7561 acgacaccag aaagtagcct aaaactaaca aaagacaaaa attccttaaa taagaacttt
     7621 atttcaattc ttttactttc aaacacaaaa aacttattag taacataagc aaataaaaca
     7681 gcaagtatcc atgccaaagc atttgcaacc ataaaattaa ataaaataac tctagtaaaa
     7741 aataaatacg aaactatatt tacaagagta gtaaatgctc caaaaaataa atataatatt
     7801 gtctctttat acttttttaa aatcaaatat aatcctccta agctaattta atataaatat
     7861 acttaaagat actaacaaac attagtatag c
//
LOCUS       DAENCV010000159         7775 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAENCV010000159 DAENCV010000000
VERSION     DAENCV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.162"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(221..869)
                     /locus_tag="KQD82_003397"
                     /pseudo
     CDS             complement(221..869)
                     /locus_tag="KQD82_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891536.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            1005..1769
                     /locus_tag="KQD82_003398"
     CDS             1005..1769
                     /locus_tag="KQD82_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381845.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISDISRNALINYENDKRIPNI
                     DTLSKLAKALKIEKTIFYFDIENEVITNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            2020..2510
                     /locus_tag="KQD82_003399"
                     /pseudo
     CDS             2020..2510
                     /locus_tag="KQD82_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2602..3354
                     /locus_tag="KQD82_003400"
     CDS             2602..3354
                     /locus_tag="KQD82_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG4381846.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKEKLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEPTLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     GDEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            3344..4510
                     /locus_tag="KQD82_003401"
     CDS             3344..4510
                     /locus_tag="KQD82_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4381847.1"
                     /translation="MATKIANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFKYRIKDLKPKSFERYESIYRNYI
                     KDTDLVNVKLNDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKIEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDINFKNSNLTVKRTLQRTYFIDKNGDRELKVLEQEPKTSNSYRTVPIPKNILNKLKE
                     HKVSQNTDILKAGELYRNDNYVFCNKLGVPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENNVPPKAVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            4609..5868
                     /locus_tag="KQD82_003402"
     CDS             4609..5868
                     /locus_tag="KQD82_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG4381848.1"
                     /translation="MIECIPYPGYRDRDGYGQINSNKEGTLRAARIVLEERLQRPIRN
                     GYQAHHTCYNRCCVNPSHIEERKVSENILDKYFNDRPEINEIVLTKESVYLTKYLLNN
                     MNNSEFNYSIEEIAKFLSIPLELLLYIKKGSVWNYIYLTNAEKVEALHKLEIFFPKEV
                     CIEANWGSEKYCPVVHKNNKQIKASRYSYDTYTNTSSSGKYILHNCDNPLCIRASHLR
                     TGDQKDNMNDKVQRRRTNRDYYLYNQEIIKKIVQLRDVENYSFSKIGREIAILVKRDK
                     PYDHKTVISIYKNYKYTRREELKEKPPTQRELLNKHIDTLIDLKHNYNYSLRQISHKL
                     GSNLIGKPFDLNVIGQVYKEHISKQNLIPPKSSIKLLEENITEILSMLDCNISLREIA
                     NRLTKKLNPNKPFHHNQITKVVNKYKK"
     gene            complement(6534..6883)
                     /gene="ssrA"
                     /locus_tag="KQD82_003403"
     tmRNA           complement(6534..6883)
                     /gene="ssrA"
                     /locus_tag="KQD82_003403"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7041..7496)
                     /gene="smpB"
                     /locus_tag="KQD82_003404"
     CDS             complement(7041..7496)
                     /gene="smpB"
                     /locus_tag="KQD82_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG4381849.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         3072 a         1068 c          966 g         2669 t
ORIGIN      
        1 ttttacttaa aaaacaagta catagattaa tatatttact actttaatat aaacatgcta
       61 tatataactt ctaaaatgat actatctaat acccactcta ctaatacaca ttttttattc
      121 ccatctagta aaaccttttc taacaattta ttaatctctc taatcacttt gcaatatcct
      181 acataactta tctttttcta aaatatctct atgcatatgt acttaattga gcaaattgaa
      241 caactgatat attaaatttt aaagtaatat tttttacttg ctctgataag tcagcaaatt
      301 tagtttctcc tatttcttta gcttcataat ccaaaatttg caaataatca attattacta
      361 aactagattt aatactcttt atttctcgct ttatccctga tattgtattt acagagtcat
      421 ttatataaag attattttct tgatttatta tacttaaaac ttgtatatat ttgactattt
      481 catcatcatc taagttttta ttcttcattt ttacaattga tattccagtt agtgatgcta
      541 tatttctaaa caaaatctgt atattactca tttctctact tataaaaaat actttcttac
      601 cttgaagtat tacatttcta agtatttgta aagctaatgc tatttttcct cttctacttt
      661 taactgctat tggagttagt tcaccaccat ataaacctcc tataatattc tctaggaact
      721 ttattctagt cttagcacct tcatttattt catcatttat attattcaaa tgctctctct
      781 gatatagccg ttatacttga atcctttttt attaatttct gattagtttt atttatatca
      841 ttttctaact gatatataag agtattatca ccttttctag tttacatttg taaactttat
      901 attttaatta taatgtccat atatgtatat acccaataaa aatatattat tcttctgttc
      961 tcatttgtgt acttttagga tatactttat acgaggtgat aatattgcaa attggaaaaa
     1021 acttaaaaaa gataagaaaa caaaaagaac taacacagat tcaacttgct gaaattagtg
     1081 acatctcaag aaatgctcta ataaattatg aaaatgataa aagaatacct aatatagata
     1141 ccttatcaaa actcgctaaa gctttgaaaa ttgaaaagac tatcttttat tttgatattg
     1201 aaaatgaagt aattactaat gaagatgttt tatacttttt acagtcttta ttcccagaag
     1261 aatactattc ttttgatgaa tttttaaatg ctatttcaaa tcttatggaa gatgatgata
     1321 ttagaattac ctcaagttta ttgctaggtg cttggtctga taaagaccaa tattataaac
     1381 ttggtgaaaa attagaacta aatgaaaatc aagtttataa ttggatactt tcagattgta
     1441 tatctaatac tgaattagat ttttcattac atcctgatga cataaaagaa attattaaaa
     1501 atgatgttct tacaaaacaa tcaattgatg aattattaga tattggtcta tcagaagaaa
     1561 atatagaaac aatctatgat tactttagtg actcaacaga taatggaagt aatattcgac
     1621 taaaaaaatt aatatctcaa aaagaaaata ttactattaa aaataatgat tactttaaat
     1681 tattacaaga aaataacgaa ttaaaggaaa aaaatgaaaa gcttgaaaat acacttaaag
     1741 aaataaaaaa actactaact aaaatataaa ataatatcaa tactatctaa agcaatgata
     1801 aaaaactatg aatggagtta caattatgaa taaatcatat atgctaaaat acacatataa
     1861 atgccctact aatttatttc atggagataa aaaatatact ctgatataca ttaaaagaat
     1921 tatgagtaag ttagtatata acatagataa tctagaaaat aactccttta tatgtgtaaa
     1981 aatataatca ttgtttgatg atatatttaa ggtggaatta taataaataa tacaattgga
     2041 gaaaagatta aacaatatag gaaatcgata aatatgtcaa ggaatgaact aaccaacaag
     2101 attggttgtt cagttcatgc tatagctaaa tatgagcaaa atcaaagaat acctagttta
     2161 gatatgataa gaaaaatagc tactgcttta tcaattgcta taaataaact cattgttgaa
     2221 gaaaatgatt tagatacttt aacaaataat aatattaatt taaaagtatt tgataaagag
     2281 ttggatttag taattcacat attagaaaat tatggttatg atataaaaac agactataaa
     2341 aaaagtttga aattacagat aatgcagata caataaaacc aattaaccta aacttctctg
     2401 acttcaaaga cttctccaaa aatctaaatt gggtgataaa tggtcttgtt gaaaatttta
     2461 tacaagaaaa ctctaatggt cttattgaag atgaggatga agaatattag tattgcatat
     2521 tttcaaaata taggtttacc tattaacttt tatacattca tagttaataa ataaaaatta
     2581 gatatgaaag gagctaaaat catgaataaa tcatatatac caaaatacac acataaattc
     2641 cctactaatc tacctcatgg agataaaaaa tatactttaa catacattag aagaatgata
     2701 agtgaatttg tatatgatat gggtaaccta gaaaataacc ccttcacatt cccagaagta
     2761 caaacattac ttgatggtat aacagtaggt ggacataaat taagtgacca aaatcaaata
     2821 ctaaatataa aatcatcatg ggattatgta ataaaattaa gtaacaaaga aaaattatct
     2881 ttaaataaag atacaatttg ctctatacat aaaatagttg caaaagatga agctctaatc
     2941 gttggagatt tcagaaatgg taatataggt atagctggta ctacccaata tgagtgcata
     3001 gaacccactc tattaaatga tttatttgta aatgacataa atatcataaa taaaataact
     3061 aaccccttag aaaaggctat aataataaat ttatggttgt cctattgtca attcttctat
     3121 gatggcaaca aaagaactgc tagacttacc tcaaacctaa tacttataag taatgatata
     3181 ggagttctat caataccagc taaacataaa caagaataca acacattaat gctaaacttc
     3241 tatgaaacac tagaaggtga tgaagttata aaattcttat tagagaaatg tattacattc
     3301 tttgatgaat ataactataa atcatataag gagctgttta aaaatggcaa ctaaaatagc
     3361 taatggagaa ggctctatcg gaaaatataa agatggttgg cgttcccgta taatgattgg
     3421 ttacaatgaa gatgggaaac caataagaaa agaattttat ggtaagactc aaaaagaagt
     3481 taaagataaa ttagatgctt ataaaaaaca atattattta agctctgata tttcagatga
     3541 taaaataact ttagagcatt ggttttacaa ttggttattt aaatatagaa taaaagattt
     3601 aaaacctaag tcatttgaga gatatgaaag catctatcgc aattatatta aagatactga
     3661 tttagtcaat gttaaattaa atgacttaag agtatctcac atacaaaaat attacaatag
     3721 attgcttgat tccaataaaa gtatacctac tataaggcaa ataaacacta aactgaaaac
     3781 ctgcttatca gaagctgaaa aacaaggtct tatacaacgt aattattgta ctatggtaaa
     3841 tataccagtt gagaaaaagg aaaataagat tgaaatattg actcttgaac aacaaaagag
     3901 ttttataaaa gctatagatg gacataaatt agaggtatta tttttacttg ctcttggtac
     3961 tggacttaga ttaggagaat tattaggact taaatggttt gacattaatt ttaagaattc
     4021 taatttaaca gttaaaagaa cgcttcaaag aacatatttt atagataaaa atggagatag
     4081 agagcttaaa gtattagagc aagagccaaa gacatcaaat tcttacagaa ctgtacctat
     4141 tccaaagaat atcttaaata aattgaaaga acataaagta agtcaaaata cagatatatt
     4201 aaaggctgga gaattatata gaaatgataa ttatgtattt tgcaataaat taggtgtccc
     4261 tatagatgat aaaagacctt taaggaatct aaaatctata ttaaacagtc tagatataga
     4321 gcctataaag tttcatggtc ttaggaaaac ttatgcgact agactttttg agaataatgt
     4381 ccctcctaaa gctgtacaag ttttaatggg tcattatgat atatcaataa ctttaaatat
     4441 atatactcaa gttatggaag ataagaaagt tgaagctgta gaaaagttag ataaaatatt
     4501 ttcattgtaa aaatcaaaca cataaaaata ctatgatttc atatagttaa ttacaaaatc
     4561 aaaataatga tacaaattaa tttaatatta ataaatgagg tgataaaatt gattgaatgc
     4621 attccttatc caggatatag agaccgtgat ggttatggtc aaattaattc aaataaagaa
     4681 ggtactttaa gagctgcaag aatagtacta gaagagagac tacaaaggcc aattagaaat
     4741 ggataccaag cacatcatac ttgttacaat cgatgttgtg ttaatccaag tcatatagaa
     4801 gaacgtaaag tatctgaaaa tattcttgat aaatatttca atgatagacc agaaattaat
     4861 gagatagttt taactaaaga atcagtatat ttaacaaaat atttattaaa taatatgaat
     4921 aactctgaat ttaattattc aatagaagaa atagctaaat ttttatctat accattagag
     4981 ctattactct atattaaaaa aggtagtgta tggaattata tttatttaac taatgcagaa
     5041 aaagtagagg cattacataa acttgaaatt tttttcccta aagaagtctg tattgaagca
     5101 aattggggaa gtgaaaaata ctgtcctgta gtccataaaa ataacaaaca aataaaagca
     5161 agtaggtact cttatgacac ttatactaat acttcaagta gtggcaaata tattctacac
     5221 aattgtgata atccattatg tattagagca agtcacttaa gaactggcga ccaaaaagat
     5281 aatatgaatg ataaagttca gcgtagaaga actaaccgag attattattt gtataatcaa
     5341 gaaatcataa aaaagattgt acaattaaga gatgtggaaa attactcatt tagtaaaatt
     5401 ggtagggaaa ttgctatttt ggtgaaaaga gataaacctt atgaccataa aacagtaata
     5461 agtatttata aaaattacaa atatactcgt cgagaagaac ttaaagaaaa acctcctaca
     5521 caaagagaat tactaaataa acatatagat actcttattg accttaaaca taattataat
     5581 tattcattaa gacaaatatc acataaatta ggttctaatt taataggaaa accctttgat
     5641 ttaaatgtga ttgggcaagt atataaagaa catatatcaa aacaaaattt aatacctcca
     5701 aaaagttcta taaaattact agaagaaaat attactgaaa tattatctat gttagattgt
     5761 aacattagtc ttagagaaat agctaataga ttaactaaaa aattgaatcc taacaaacct
     5821 tttcatcata atcaaattac taaagttgta aataaatata aaaaataaca ttttaaccac
     5881 tatttttaga aaacgcgtgt gaaatagtgg tttatttaaa cgtatctaca gttcttttaa
     5941 ttttacaaaa ataaatatta aataattaaa ttgattttat atcaattcta aaaaacaact
     6001 atctacatac acctttttac ctcttctctt aaatcattcc atcctgttaa tcatctgcgt
     6061 ttttatacta atcacataaa atcttttacc aaaatttaaa gaacctcttt agaatataga
     6121 atcagaagtt tttgctatac aataagaact atgatatgca atcatcataa cacttccttt
     6181 tatttaagtg gttctattaa atcttctact atgtaacttt tactttaaaa atgtaatgat
     6241 tcataacaag gtatttgaaa tatcagtgtt atgtttaagt attttataaa cttcttctga
     6301 gttcatattt aaggcttctg ataaagctcc ctaaagtatg tattaaattc ctatcctctc
     6361 taatgtaatg ataagtaaat gttttcatac actcaaatgc acccccatat actctcaaaa
     6421 atacctcatt ttctgtggtt ggggtacggt tggtggattt tatctccaaa ataaaaaata
     6481 ctagtcaata aagactagta tttccaagta tttcatcatt cttttttcaa aattggtgga
     6541 gacgagggga gtcgaacccc tgtccgcaaa tcttttcctt gctgcatcta catgcttagc
     6601 caacttcact aatatctcac ctcacatagc gccaagtggc aggctctatg cttagccagt
     6661 cccaattaat ctacttcaag tcgagacacc cctaaagtag ttccctgcat aatgacgtcc
     6721 agtcttagtc ggcaggagga ctaagaggga cgagcagcaa ctaggctgct atcgcgaaat
     6781 tatcgtctgc gtttatttta aagttcccca gtttttacgt gacccagagt aacacgacat
     6841 gcagcctcaa gttccaaacc cacgtcgaaa cctttacgcc cccttgagta gtttatagtc
     6901 cagtagactt ttcgcaaact ttatcaccat ttttattaaa tttttaatgt atatataata
     6961 taatactttt aagaataaaa atcaatgtta tgttctagat aataaagaat tttattctaa
     7021 aaaatacagt tttatctagt ttaatactta cctgtcattt ctctttctat acgtctttgt
     7081 gcatctttct tagccatatc ttgacgttta tcatatagtt ttttaccttt tgctaaggca
     7141 atctctactt taactttacc gttttttaga tacaaactaa gtggtataag tgaatacccc
     7201 tttatagtag ttgctccaat aagctttctt atttcatgct tatgaagtaa taattttcta
     7261 actcttaaag aatcaacatt gaatatattt ccttgctcat atggacttat atgaacttgt
     7321 tttaaaaata cttcgctatt atctacagat gcaaatccat cacttaggtt aactttgcct
     7381 tgtcttattg attttacttc tgttcctttt aattcaattc cacattcata agtttcttct
     7441 ataaaatatt catgtcttgc tcttttgttt gttgctaata tttttttacc agccatataa
     7501 atctcacctt ctttgctaga aatttcttcc ataaatatta gaaatttact aaatatttaa
     7561 tgttgccaaa ttatttgact aacttgtatg ataacatatt taggcatttt agtcaatatt
     7621 tcactgatat attaagttag ttaggctata tataattaag taatataaat gtatgaaaat
     7681 agctaaaaaa ctacttcgaa tatacttaat actaaactaa ctctaaccag taatactaat
     7741 ttataaattt caaaacaaaa atattacata ttata
//
LOCUS       DAENCV010000160         7772 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAENCV010000160 DAENCV010000000
VERSION     DAENCV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.163"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            932..2290
                     /locus_tag="KQD82_003405"
     CDS             932..2290
                     /locus_tag="KQD82_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG4381850.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSILLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2397..3191
                     /locus_tag="KQD82_003406"
     CDS             2397..3191
                     /locus_tag="KQD82_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG4381851.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3184..5490
                     /locus_tag="KQD82_003407"
     CDS             3184..5490
                     /locus_tag="KQD82_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG4381852.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5629..6906
                     /locus_tag="KQD82_003408"
     CDS             5629..6906
                     /locus_tag="KQD82_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4381853.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7089..>7772
                     /gene="yqeC"
                     /locus_tag="KQD82_003409"
     CDS             7089..>7772
                     /gene="yqeC"
                     /locus_tag="KQD82_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG4381854.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIF"
BASE COUNT         3061 a          841 c         1448 g         2422 t
ORIGIN      
        1 atacaaatat agtaaaggag agtacattgg tacaactgag atacatagct tattaagttt
       61 ttctgtctta tgagaattat attataaaat attcataaat attttgagtt taaattaaat
      121 aattatttta tgcttagatt aattgatatt taattaattt aataacaaaa acagtgaaag
      181 agtaaataat attatttaca gggggtaaag caaagtatgt taaataaatt gtacttttgg
      241 ccccgtacat agagaaatct atgtaggaca caactttaaa accagtaaat cctaaagcct
      301 tgcaactaca ataaaagata gaaagaaact tttatgaatg tggtgaaaac cagaaacaat
      361 agtgaggatg gtatatggtg agagcctaag tacttaaaaa tggacatttg gtcgccaagc
      421 ctagaaatag gaaggtcaaa cgactatctc gagagagagt acactataag cgattgatag
      481 tggaaacggg ttgctcttag caggtaatgc tgaagatgaa gaaatagtct acgctcatat
      541 gaaagtatga ggtcactgga tttgaagtga catctataaa gtagcgttta taggtaaatg
      601 tttaaaactt gaaacatgta aatacttatg aaaacttata acttataatg attaagattg
      661 aaagatttaa attgaattaa atttactata aaaaattaaa aattcatagc tataatactc
      721 ttatatttgt aaagcatggt atataatgta tacaaaatta aagaatacgt ttatttgtta
      781 ataattaaat ttattgcatc tgttttttaa gactaaaaac ttaaatatat gttggagtat
      841 agttgatatt tatgaacatc attaatttaa tatattagat ttttaagtat ttatataatt
      901 caataagttt tatgtaacgg gagtttttat tatgaaagca aaaagtacat caaaatttga
      961 attagacgga ataccaccac taaaggaagc aataccttta gggttacaac acttaatggc
     1021 aatggtaata ggtagttgtt taccagcgat attgattgca aatgcagctg gacttaatca
     1081 taagatgtca atattattga ttcaagccgc attagttttt gcaggattat caactttatt
     1141 acaattatat ccaatacctt tgtttaaagg ttttaaatta ggaggaggaa taccagttat
     1201 aatgggagct acagctatgt ttataggagc tggggtatct gttgcaagta agtatggact
     1261 accagtatta tttggctctc aaataatagc tggtatagta ataattataa ttggttttgg
     1321 tcttaaaaaa atacgattta tatttactcc agctgtatca ggaacaatag ttgcatgtat
     1381 gggtcttgga ctttttccaa tagctataaa aaacttagct gggggtatgg gcaatgagac
     1441 ttttgggaat ccaattaatt ttatagttgg aatatctgta tctcttatga tattaggatt
     1501 aaataagtat ggaaaaggat tatttaaaga tgcatcaata ttagttagca taatttttgg
     1561 atacatttta tcattaattt tgggtatagt aaatttttct tcaattcagg agtttacttt
     1621 ggttgcactt ccaaaaccac ttgcttttgg gctagatata agacttgaag ttgttgtaat
     1681 gttttcaata atatatttag ttgagatagc tgatataatg ggtgcttgta cactttctgc
     1741 ggtaggaggg cttaatagac aagtaacaga tgaagaactt tcttctgctg ttttaggaaa
     1801 tggagtactc tctacaatag gtgcattatt ttctgcaact ccaatgggga tgtttggaca
     1861 aaattctgca atcgtttcca atacaaaggt tgtaagtaaa tttgtactag ctattggagg
     1921 aattggatta tttttggcag gaatatcgcc tttattagct aatttaataa gaacaattcc
     1981 tccctgtgtg gttggaggag caacactcgt tatattttca actcttacaa catctggttt
     2041 aagacttgtt agtatggatg gatttaatca agaaaatagc atgatattag gtctttctat
     2101 ggcttctggt attggattca tggtagctcc tcaagttctt gaaaaatttc caaaatttat
     2161 agagacatta cttgcagatt caagtgtagt ttcaggagca atggtagcca taataattca
     2221 agctttatat atggtaaaat ttcctggaaa taaaagtgat aaggttgagg ataaaaataa
     2281 aagtttataa tgtaaaggtt taatatatgt gaattaaata tactacaatt ataaatataa
     2341 ataaaagatt tattaaaaac ttttgtgttt taataagtta atgatggggg gataagatgg
     2401 ttactataaa ggaatattca gttccacaga gtttagaaga agcatataaa attttaattt
     2461 ctaaaaaaaa taatgttatt ttaggtggat gtggatttat aaaattaagt aataaaaata
     2521 taggaacagc gatagattta aaagatataa atctaaatta tataaaagag gataaaaaaa
     2581 atattttgat aggtgcagat acttcactta gaagtttaga aattgataaa actataaaaa
     2641 attactgtag tggaatactt tcaagcgctg tttctaatat agttggtgtt caatttagac
     2701 aaggggctag aattggtgca agtgtatttt caaagtatgg cttttctgac ttaataccag
     2761 cgcttttggt tgttggagcg aaggtgagat tatacaataa aggaatacta gagttaagtg
     2821 agtttttgga tagtgaatta agtagggata tattggtcga agtaatacta ccaaaaaaag
     2881 atgcaatagc tgtttttgac tcaataagaa aatgcacagg agatttttct gtattaaatt
     2941 cagcaatgct aaaagaaaat gatacttaca aaatagcaat tggggcaaga cctcaaaaag
     3001 ctagattagc acttgaagcg agtaatatat taaataaaga aaaagatata gataaggcat
     3061 ctattgttgc tagtaaggaa cttacttatg gaagtaatat gagagctagt aaagagtaca
     3121 gaaaggatat ggcaagtgca ttagttaaaa ggatgtataa tgctattgag gagggaatgt
     3181 ataatgatta ataaggggat aagaaagata gatagtacag caattgtcac aggaaaacct
     3241 ctatatactg aagacctcat tatgcataaa gatgttttga cattaaaact acttagaagt
     3301 ccacatgctc atgctaagat aaagaatata gatacatcaa aagccgaaaa aattccaggg
     3361 gttgtgggta tttatacgta taaagatgta ccacaggtca gatattgtgt gccaggtcaa
     3421 acagaaccag aagcatcacc ttatgataga gttttacttg aagatacagt tagatttatt
     3481 ggagatgaag tagctttagt ggctgctgaa gatgagaaaa cggcacttaa agccttaaaa
     3541 atgataaagg tagattatga ggttttaaag cctgtgtttg atgcaagaga agcagaagga
     3601 aatgaagttg ttatacatcc agaaaaagac atagtagagc catacccttt tggattggat
     3661 gcaaaaaata atatagtatc aaaagaattt tttgaatttg gagatgttga agctaagctt
     3721 aaagaaagtg attttgtggt tgaatcttca tacaaaaccc aaagtcaatc gcattgcatg
     3781 atggaaactc ataggtcata ttcatataaa gatataaatg atagaattgt tgtagtttca
     3841 tcattacaat caccacacaa tgtaaagaga atacttagaa aagtattggg tataccatct
     3901 tcaaaaataa gagtaattaa acctagaata ggtggtggat ttggaggaaa gaatatagca
     3961 gcaacagata tatttactgc atttgttact atgaaaactg gtagaccagc aaaatttgta
     4021 tttacaagaa gtgaaacttt tgtaggttct aatagtagac atgaaatgtt ttttgatgtt
     4081 aaagttggtg cagataaaga aggaaattta aaagcaatag agctaaagtc attaaacaat
     4141 acaggtgctt atggtgggaa tggtgtctct gtttcttccg aatctggaca caatacctta
     4201 ccaatatata atgatattga tgctgttaga tttaatgcta gaacagtata tactaatagg
     4261 ttaccagcag gagcacttag agggtatggt gctacacagg gaatatttgc aatagatagt
     4321 gctattgatg aattggcaca taagattgga atggatccac ttgaatttag aattaaaaat
     4381 ataatagata ggtataccaa tggaaaagca gctgaagacg aaatattaag ttgtgagctg
     4441 agacaatgta tagaaaaggg caagaaatta attaattggg atgataaata tccatgtaaa
     4501 gatataggaa acaacaaagt aagagcagta ggtatggcag tagcaaatca tggttctggt
     4561 ataccgcgtg tagatatggc aacagtcact ataagaatgg atgaagatgg cacatataaa
     4621 ctattatctg gtcttgctga cttaggtcaa ggggctgata caattcaagc tcaaattgct
     4681 gcggaagctt taaatactac tgtagataga atttctattt atacaggtga ttcagactta
     4741 tgtccctatg atacaggagc atttgcttct tgtacgacaa cggttacagg aaatgcaaca
     4801 ataaagacag caaataaatt aaaagatata ttactttcag ttgcagagtc aaagctagga
     4861 tttaaaaaag aagatttaga gttgggaaaa gacagggttt ttgttaaaaa cgataatagc
     4921 aagtttgttc tattagagga attgggtaga gaatctgttg gaggaatagg atttgctagt
     4981 gatgatgtac cagtagtttt ggaagcaaca gaatcttttg gcatgttgaa aaaaataaaa
     5041 ccatttatag caggatttgc agagatagaa ttagataaaa atacaggcga atataaagtc
     5101 ataaattttg catgtgtacc tgattgtgga acagttataa atccaacttt agctagaata
     5161 caagtagaag gtggtgtgat gatgggtata gggtttgcaa tgtatgaaga ccctatattt
     5221 gataaagatg gtagattatt aacagatagt ttcctacagt ataaagttcc tacaaaaaaa
     5281 gaagttggaa atatattggt agattttgct gagaatttcg acccaggagg tccttatggt
     5341 gctaaatcat taggagaaat agttatacat acaccagcac cagctatagc aaatgcaata
     5401 tttaatgcta caggagttcg tttaagagag cttcctatga cttttgaaaa agtttatatg
     5461 ggtatgaaaa ataataataa acaattataa taaataataa gatgtataaa agcaagtaaa
     5521 tttaaattat tttgtaaaat aaagtaatta aatggacatt atatatggtg ttttgtaaaa
     5581 ataccgtata taatgtctag tagagtatat tctaaaggag gtgagttcat gtcaaacaga
     5641 ataaaaaata tacactatgg aactcagaga caacaattaa aaaacttaac taaaaaagaa
     5701 tatcaagctc tacgagaaat gtgcttctta gctaagaact tatataatgt tggtacatat
     5761 aatgtaagac agcattattt caatcaagga gaacatctta actatgaagc taattatcat
     5821 gtatgcaaag aaaatgagaa ctataagctt ctcaatagta atatagcgca acaaatatta
     5881 aaagaagtag acagttcgtt taggtcattt tttggattaa ttaaattagc caaagaagga
     5941 aaatatgact ttagagggat aaaatcacct aaatatttag ataaagaagg attttttagc
     6001 ataataatag gtcaaataag aatcaaagaa gatgggattt taaatgtacc tatgtctcca
     6061 gcatttaaaa aactatatgg gaaagtaagt ataaaagttc cacaaaatat actagagaaa
     6121 aaaattaaag aaataagaat aatacctaaa cataaagcta ggttctttga aattcagtat
     6181 tgttatgaaa taccaaaatc agaggaagtt tcaaatcaaa aaaatgcact agctatagac
     6241 ttgggtttag aaaatttatg tacatgtgtt accaatttag gtgatagttt tatcatagat
     6301 ggaaaaaggc taaaatctgt gaatcagtgg gcaaacaaac agaatgataa aatagaagcc
     6361 ttaaaattaa aaaagaatat aactgcaata acaagaaaac aatttaaagt ttggaataag
     6421 agaaataatc aagttaaaga ctatgtaaat aaaacctgtt tttatataat tgattattgt
     6481 gtaaaaaata atattggaaa tttaattgta ggatataatg ctaagttaga aaaaagtgat
     6541 aatatggaga gaaaaatata tagaaatttt ataagaatcc catttggaga gataaaagga
     6601 aaattagaat atctgtgtaa aataaaaaat atcaatttta ttaggcaaga agaaagctat
     6661 actagtaaat cagatttttt tgctgatgat tttctacctg atatcaatgt ggacaatgtt
     6721 aaaaaatatg actttaaagg ggaaagaata acgcgtggac agtacaaatc cagtgttgga
     6781 atcattataa atgcagatgt aaatgcgagt cttaatatac taaaaaaatc acaagtagtt
     6841 aatttatcta atttacagaa taatcctcaa atattaaagc aaccacagag agtaagaata
     6901 tcttaattat atttaaaaga taaactgata gcgtaagctt acagcttagc ggcgaattgg
     6961 atccgccttg ttgtttacaa cagaatctat ttactttcga gtttatggaa tttcaaaaat
     7021 cacttttact aaaaaggtct aactataata attaagagat tagaaaactg gttataagag
     7081 gtgaaagtgt ggaactatca aatataatag aaaagaatga aataataact gtagttggag
     7141 caggaggaaa aacttcgctt ataaactatt ttgcaaattt ttatagagat aaattgaaag
     7201 ttctccttac aacaacaact aaaatatatg taccaaacga ttatgacaat ataattatta
     7261 caatagatgg aactgttatt ccatctattt gtcatggaat aacagtctgt ggcagttata
     7321 taaataatga aaataaatta gtaagtattg attcctctat tttggatgaa attgtagacc
     7381 aatttgattt agttttaata gagggagatg gctcaaagag aaaaaaactt aaaggttgga
     7441 atgctaaaga accagtagta tatcataaaa ctacaaaaac catagggata ttagatataa
     7501 catcatttgg aatgaatatt aatgaagaga atatacatag agtagaaata tttaaaaaaa
     7561 tagcaaattt agatacaagt agtattaata gtagttctac agtaagtatt gaaaacttaa
     7621 aaaatatagt attaaatcca aatggattat ttaaaaatta ttctggaaaa agagtattat
     7681 ttattaataa agttgaaaat gaaaaatata aaaatttagc tataaaatta atagaaaata
     7741 ttaaagaata tgaaagtgaa atagagatat tt
//
LOCUS       DAENCV010000161         7718 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAENCV010000161 DAENCV010000000
VERSION     DAENCV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.164"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            219..1520
                     /locus_tag="KQD82_003410"
     CDS             219..1520
                     /locus_tag="KQD82_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4381855.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1596..1871
                     /locus_tag="KQD82_003411"
     CDS             1596..1871
                     /locus_tag="KQD82_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4381856.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3313..3543
                     /locus_tag="KQD82_003412"
     CDS             3313..3543
                     /locus_tag="KQD82_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381857.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3536..4117
                     /locus_tag="KQD82_003413"
     CDS             3536..4117
                     /locus_tag="KQD82_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4381858.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4695..5372)
                     /locus_tag="KQD82_003414"
     CDS             complement(4695..5372)
                     /locus_tag="KQD82_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG4381859.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAIQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5561..6925)
                     /locus_tag="KQD82_003415"
     CDS             complement(5561..6925)
                     /locus_tag="KQD82_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG4381860.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLIVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7053..7574
                     /locus_tag="KQD82_003416"
     CDS             7053..7574
                     /locus_tag="KQD82_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG4381861.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3009 a          873 c         1333 g         2503 t
ORIGIN      
        1 caaataagat attgctatta tgtagtaaat tatataaaac aatgtatcag aataaatagt
       61 atattaataa tttgtagtat atcatgacga ataatacagt cataacaaat agtgagtcat
      121 aacaaataat gtatttataa ttgataatgt atttataatt gataatgtat ttaaaaatca
      181 aaatataaaa acttataagg aaagagagat taatttatat gggtaaatca aaagatgttg
      241 aaatagagaa gtctatttta ggcacaattc ttttagacaa taaattaagc tataagctag
      301 atgaattaaa tgaaaacatg tttatgaatg atatatgtct tgagatattt aaaattatga
      361 aagaacttaa aaaagaaaac atagtgattg atgtggccac tgtgaaatca aagatagata
      421 gaaaatctct agcaataaaa acaagtgatg tgactaatct aattacttgg ggtcagaatt
      481 ttggtcttga tggtcatatt aaaattttaa aagaaaattt agctagaaga agcataaatc
      541 aaaattgcca aaacctttta cacagcctaa accttggaga aaatatcgat acttgtattt
      601 acaaatttga aagtaatata aaggagattt tggacaagga tacttatgaa aatgatgatg
      661 taaatagtat agcagggaag gtgcttgatt ttttagaaaa caaaaaagat attggattta
      721 aatttggtat aaaattactt gataccacta taggaggtct ttttaaagga gagctaacca
      781 caatagcagc taaatcgggg gttgggaaga ctgctttggc acttcaaata atgttaaatt
      841 catttaagca aggtaaaaaa actttattta taagtagaga aatgaccagt gagcaagtat
      901 ttatgagaaa tatatgcaga gtaactggtg taagtacaag gaatatgaaa tctaaagaaa
      961 tagatgaaaa tgattggaaa cttattgtaa atgctatagg tgatttaagc gaaaataact
     1021 taatttatat caatgataag atagatacaa ttagtgcaat tagaaagagg ataaggcagg
     1081 taaagccaga tttattaata gtggattatg tgcaactcct tacatctcag aaaagcatgg
     1141 ataagagaga aagggaagta gctacttttt cgagagaact taaaaatatg actttagatt
     1201 ttaatatacc tgtaatccaa ttaagtcagt taaatgatga aatgaaagat tcaagacctt
     1261 atggagatag acctatgaga gatagcaaag ccatctacca cgatagcaac aatgtggttt
     1321 atatacatca attaaagggg agtgattatg aagaagcagt gagagatatt ggagaaagtg
     1381 aggaggcagt tagagcatca gaatacaggg gaattaagat ggttgattta atagttgcga
     1441 agtgtagaga tggacagact aggcataagc atttttgtta ctttggggat aagttacatt
     1501 ttcaagaact aaattattag gtcaataaag tagtaataac aagcttatta aaagtcgggg
     1561 aatagcaaat agattataaa atatgaggtg aaaatatgtt ggaattaaca aaaattatag
     1621 aattacttga agaacacaag atagaagtaa gtattccaaa ggagttacat aagtatagtt
     1681 tattggaata ccttgaattg tgtcaacaaa gtgggggaac atatttttat attaaaacac
     1741 tggaaggggc tttaaaagag gctagaaatg agcttatgat taaattgttt aatgggaaaa
     1801 attatatgga attagcgagg atgtttgaaa tgagtgttat taatgtcagg catattgtta
     1861 gaaaaaaata gatatttttg gagaatacaa gtgtgatata cttctgaaat agaagttatt
     1921 atagttgaaa taaaattgtg gttaataaat taaagttgtg tataagttta agattataga
     1981 ggagtataag tgtgattaac agcatgattt tgtgaataaa attgaggata tatatgagtt
     2041 aaatgtgtga ttaataaggt aaggttgtgt ataagattat gagtatttaa aaggaggggc
     2101 tataattaat agtgtagagt tgtgtgtttg cttaagatta tataataatt taatagatta
     2161 aactatacta ataaggtaaa atcgtgtgtt tagttaaagc ttattataaa atcttgatat
     2221 gtgattaatg atgcaatatg tgaataaagt taagattata atagatttaa cctgtggcta
     2281 atagaataaa attgtgaata aagttaagat tataatagat ttaacctgtg gctaatagaa
     2341 taaaattgtg aataaagtta agattataat aggtttaatc tgtgactaat agagtaagag
     2401 tgtaaataga gataaacttc aatatgagct taacctatga ttaatagagt aagagtgtaa
     2461 atagagataa acttcaatat gagcttaacc tatgattaat agagtaagag tgtaaataga
     2521 gataaacttc aatatgggtt taacttgtga ttaatagagt agggtgtaac taaagataaa
     2581 cttcaatatg agtttaactt gtgattaata gagtaaggtg caactaaaga taaacttcaa
     2641 tatgagctta acctatgatt aatagagtaa gagtgtaact aaagataaac ttcaatatgg
     2701 gtttaacttg tggttaatag agtaagagtg taaatagaga taaacttcaa tatgggctta
     2761 acttgtgatt aatagagtaa gagtgtaact aaagataaac ttcaatatga gcttaaccta
     2821 tgattaatag agtaagaatg taaatagaga taaacttcaa tatgagttta acttgtggct
     2881 aatagagtaa gagtgtaaat agagataaac ttcaatatgg gtttaacctg tggttaatag
     2941 agtagggtgt aactaaagat aaacttcaat atgagtttaa cttgtggcta atagaataag
     3001 attgtgaata aagttatagt tttagatggg tattaacctg ttactaatag aatatgatta
     3061 ggtgtaaaat tatagagctg tataagaaaa tattatagat atattaaata ttagataata
     3121 aatatttata tagatacaca aaagaaaagg cgatatgaag ctaaacttaa tgacttcata
     3181 tcgtttttta tttgaaactc ctttgtattt agagtgtgat ttttaatatt gaggttttat
     3241 gtgattaagt cacagataaa aagtaagtat cttgatataa ttcttataga ttaataatat
     3301 agggaggtta ttatgtctaa taaaaaaaag aagtatgcaa atattttagc aaatgagtgc
     3361 gttgcttgtg gtagctgtat aaaagcatgt ccaagaagtg ctatctcagt tccatgtggc
     3421 attagtgcaa aaattaatag agatttatgt gttggatgtg ggatttgtga aaagatatgt
     3481 ccagcatctg taattgagat tataacaata ttaaaagagg aagaaggtaa gtgtcatgaa
     3541 taataaaaaa cattggtatg actatttatg gattttttct tcaatatatt tgatattggg
     3601 atttgtaaat attattttcg cttggattgg gctaatttgt ttctttgttc cactagctat
     3661 ttctattgtt aagggaaata aagcttactg taataagtat tgtggaagag ggcaactgtt
     3721 taatttgctt ggaaataaat tgaagctgtc aagaaataga gatattccta aatttattag
     3781 aagtaagtgg tttagatatg ggtttttgac attttttatg gtaatgtttg ctaatatgct
     3841 tttttcaact tatttagtgt ttgaaggaag tagaaatttt aaagaagtag tgactttgtt
     3901 gtggactata aaaatgcctt ggcaatgggc gtatcatgga actttagttt caccttgggt
     3961 ggtacagttt gcatttggtt tttatagtgt tatgcttaca tcaactgttc ttggtttgat
     4021 tactatgata ttgtttaaac ctcgttcatg gtgtgtttat tgccctatgg gtactatgac
     4081 tcagatgata tgtaaggcaa agagtaagtc tgtatagaaa ggccttatgt gatatttaca
     4141 gttttgttca agtagagatg agtagtaggt ttgaatatat atgacaaata tatgagtata
     4201 ggggcataga tttagtttgt gtaaggcaaa ctgatttatg cttctttttt atgtcttaga
     4261 ttattggcta agtagaaaca atgaatttag atattaagaa ttattcagtt atttgaagaa
     4321 aatagaataa taattaacat tatatgaatg gatattcatt acatataagc agttaatagc
     4381 aagatttatt taatatgaaa taggtcaagt atttaaacta aataaggtca tgacaattaa
     4441 tataagtata gatataaaaa gattagaagt tataacttat aaaaggtatt ctatataagt
     4501 agtatgtgga aaatagtcaa attaacttat tagaaacaaa aaatatacat aaaaaataaa
     4561 tgtaaatatc aaataagata ctatataaaa atagatataa gtatcttttt tattattgaa
     4621 attatgaaaa tatattttat tttacacaaa aaagagtgcc tcatgaacta aaaagttcat
     4681 tttgagacac acttttacag tgtcaaagaa cgaagtttat ccttatccaa tacttttata
     4741 ccaccacgag ataaagacac aataccttct ctagcaaaat atttcaacat tctcgataca
     4801 acttctctgg cactacccat atacttagca atttgctcat gagtcatatt tatagtgcta
     4861 ctgccatttt tagctatctc atcaattaaa aaagatgcca aacgcttatc aaaactcata
     4921 aaaagaattt gttgtatagc ccacataaca tcagaaaaac gttcggtagc taatttatat
     4981 gaaaataact ctgcatgaat attttctgta gataattttg caaaaacaga ggaactaatc
     5041 tgtataatat cgcagtccgt ttcagcatct acatagacat caaaagttat tgtttttaga
     5101 atacaggaag ctgataaaat acatacatct ccatcatcaa gtcggaacag tgtaacctct
     5161 cgaccttcat ctgaaagtat gtatgtacgc actgttccag actttataat caaaatacca
     5221 atacagtcac tatcaccaca atgtactgga ttcccttttt tgtaatgcga ggtatttgct
     5281 gaagaaatta ataattcttt ttgtaagtca gtaagtttat cccaaaaagg caagctatgt
     5341 gtaagtatat caatttcatt ttgtgaaatc atacaatatc tcctttctta aattgttata
     5401 atcccatgat acaagtgaat agataaattg acaagtagtt tgttaagagg attatttagt
     5461 cagatagtaa tagtatgatt tatgtagaaa aaatatgata atatgtattc ttgctattaa
     5521 tgagaaagta atatgttttg atgatagtag gtttgtataa ctaatgcata ccgtttattt
     5581 ttaaaagatt aagttcctta tgtaacctta tagacataaa accagcaatt atagcagcca
     5641 tcaaatcggc tattggttgt gcatataaga taccatttaa cccatagaat acaggaataa
     5701 tcaaaattat agggataaaa cagacacctt gtctacacat acctaaaaat ccaccttcct
     5761 ttgcttttcc tagtgcgagg aataatgtag aatacactgt atagaatcca aatagcataa
     5821 atgaaaaccc atttgcccta agagctttac taccattttc tattaataaa atgtcaccac
     5881 ttgtaaattg tgaaattata gattttggga agagtatcaa tataagacca agcactacac
     5941 aaaatactgt agtccataat atggaagttt ttattgctat atgtaatcta tcgtagttct
     6001 ttgaaccaaa attataacca gcaattggtt ggaaaccttt tataaaacca aatacaataa
     6061 gactacctaa agatgttatt cgtgttacag cacccattcc agctatcaca gaatcaccat
     6121 actttgtagc ctccatatta gttaatgtaa tggcaagact agtcaataat tgaaatatta
     6181 aggtaggtat gccaattttt aggatttctg aaaaaatttc ttttgaaaaa cagcattcgt
     6241 taattctaaa actaaatata cttttcttgc ttaaaatata tcctacataa acaagtgttg
     6301 aagcaatctg tgagatagct gttgcaatag aagcacccct tacccccaga ccaaaagtat
     6361 aaatgaatat cgggtctaaa atcatattaa gcaaagcacc taataacata gcacacatag
     6421 ttgttttagc agctccttca cttgtgacaa tattagtcat agtgacattg aatatattaa
     6481 aaatagatga cacgacataa atgctagcat aagatattgc ataaggcata atactttttg
     6541 tagctcctaa caattttaaa attggattga gaaaaatcat aatcacaata ataactaatg
     6601 tacccactat aatactactg taaatagatg tacttaccac ttttcctgct ttttcattat
     6661 caccatgccc taaaagtctg gcaatatatg aagcagctcc atttccaaac aataatccta
     6721 atcccacaac aagctgacct aatggaaatg caacggatat agctcccatt tgacttgttc
     6781 caagtccacc cacaaagtat gcatcaacca aattgtataa tgcagttatc atcataccta
     6841 tcatggtagg taatcccata gataacaatg cttttgagat tggggcagac cctaataatt
     6901 caattctttt gttactttct ttcatactta acactcccct tctattgaca ttaagtctaa
     6961 attataatac tataaattat agtaagtatg gaaaatagta ctataattta tagtatttgt
     7021 caatagtaag aaaactaaag gagatgattt ttatggctac aatagattta attgtattgg
     7081 gaatagtaaa gcaacaaccc aagagtgctt atgatattca aaaggcagtg gaatacagga
     7141 atatatcacg ttgggttaaa ataagtacac catcaattta taaaaaagta attcaattag
     7201 aacaaaaggg atatattgaa agtaaaactg tgaagaatgg aaaaatgcca gaaaaagcag
     7261 tttattcatt aactgaatta ggtgagacac attttttaga tttaatgttt gatacatcta
     7321 gcaaaccaat aaatattctt ttagatttta atgctgttat agtaaattta aatagcctta
     7381 caccagaaaa acagaaatta tgtctcaata atattgaaga gaatgttcaa gagttaaaga
     7441 aaactattga ggaaaatata agtataaaga cagaagtacc agagacagca aaagcagtgt
     7501 tggagcaaca gttggtgcta gcacaagcat tggaaacctg gataaattct ttaaaagata
     7561 atatgggaaa ttagaattag agaagctaac ttaataatca ttaaacaaaa accagcatat
     7621 ttaataatga gatattagaa aaaataaaac aaatgatata aactgaaaag tatatttaaa
     7681 ataaagctca cttaattgag ggagaatgtt aaactttt
//
LOCUS       DAENCV010000162         7394 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAENCV010000162 DAENCV010000000
VERSION     DAENCV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.165"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(22..684)
                     /locus_tag="KQD82_003417"
                     /pseudo
     CDS             complement(22..684)
                     /locus_tag="KQD82_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(844..1380)
                     /locus_tag="KQD82_003418"
     CDS             complement(844..1380)
                     /locus_tag="KQD82_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG4381862.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="KQD82_003419"
     CDS             complement(1453..1866)
                     /locus_tag="KQD82_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG4381863.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIIFIHDRECSECQEKVK"
     gene            2359..2997
                     /locus_tag="KQD82_003420"
     CDS             2359..2997
                     /locus_tag="KQD82_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG4381864.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3605..3784)
                     /locus_tag="KQD82_003421"
     CDS             complement(3605..3784)
                     /locus_tag="KQD82_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4381865.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4010..4543)
                     /locus_tag="KQD82_003422"
     CDS             complement(4010..4543)
                     /locus_tag="KQD82_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG4381866.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQITVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5179..5498
                     /locus_tag="KQD82_003423"
                     /pseudo
     CDS             5179..5498
                     /locus_tag="KQD82_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6128..6700)
                     /locus_tag="KQD82_003424"
     CDS             complement(6128..6700)
                     /locus_tag="KQD82_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG4381867.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6762..7025)
                     /locus_tag="KQD82_003425"
     CDS             complement(6762..7025)
                     /locus_tag="KQD82_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG4381868.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7025..7246)
                     /locus_tag="KQD82_003426"
     CDS             complement(7025..7246)
                     /locus_tag="KQD82_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG4381869.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2599 a         1257 c          884 g         2654 t
ORIGIN      
        1 agtgtgcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacccgcc agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccatgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaaccttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactatt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttactta ctaaatataa gaaaataatt gctcaatttc tcaataaatg tgaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttaattcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgctaatt tttgtttatg
      961 actatcatag agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacactat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgcgctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaata gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct atcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattctctat catgaataaa
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccattcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaagt ttaaaactaa gaataaaaag agataaaaag gaagctacta atcaaaatat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgatattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag tcattaataa
     2221 aaacgttctc aatatagata tatttaacca tgttttgaat agcagataat tatatgatta
     2281 aaaattaaat atgacatagc gtttgtggaa tataacacct aaattatgtt ataataataa
     2341 aacaggagtg tgatagtgat ggatgaaaaa atattagagt tattacagga aatgcaaggt
     2401 aatattagta atatgcaagg tagtatcagt ggtatcgaag gcactattag cgatatgcaa
     2461 agcaatatca atggtatcga aggcactatt agcgatatgc aaagcaatat caatggtatc
     2521 gaaggcacta ttagtagtat gcaaggcaat atcaatggta tcgaaaacac tattagtggt
     2581 atgcaaggca ctattggcga tatgcaaggt agtatcagtg gtatgaatgg cactattagc
     2641 gatatgcaag gcagtatcag tagtatggat ggcactatta atagtatgca agacagtatt
     2701 actgatatgc aagttgaaat taaagaagtc aatagtaggt tagacaatat ggaacctgat
     2761 attaaagact taaaaactgg acaagttgaa actagtagta gacttgatac tattcagagt
     2821 gatgttacag aaattaagtc agatgtaaaa attaacagaa atacattaga tacagtgatt
     2881 aaacatacct cttcattagt aaaagataca tcagatatta aaaatgattt aaataatata
     2941 gagatagtca catctaagaa ttcgaatgat attgctaaat taaaatcagt taaataatat
     3001 actatagtaa aaaatacctg aaaatttcac atacttcttt aaagttatac caaattttat
     3061 acagaaatac tgattatccg acaacacact ttacttataa ataatttatt aaaaaaatac
     3121 tcattttatc ctaaaattaa cacgactaaa tgagtcttga tataataata aaaaataagg
     3181 gagattggac actaaaaagt tgtgtatctc ccttatatcc tataatatag ttatataatt
     3241 tttcagccta aaataaaagt ccatctttga ttaaaacaca tccccttatc acttataaaa
     3301 ggataaaatc ttgttttcat ttttccattt atatcttctt gcttaagagt aataattttt
     3361 tcttttgata ctttaagtgt ttggttaaat ttaaccaatc gactaagttt tgttcaacat
     3421 ctatttttca tattttccaa tacttaatct attttttata aataattctt tcatttctaa
     3481 ctcacttaca ttttttacat aaaacgaagc atataaattt caaccttatc ttgtatggtt
     3541 cttaatactt aatgagtagc ttaagtatca aacattataa attttcccct tacatttaac
     3601 actcttacca tttccaaaat atacaaaata tattttgaac atttgtacaa tgatgaaata
     3661 caatacatga aaatacaata tcataactgt tgtctaaaaa tagaagttct tcaacataac
     3721 ctttcacaaa aaacatatta tctaaatatt ttttgtctgc tccttgtttt cctgtttgta
     3781 gcatataaat tactgcatct aaacatataa ctactcacac gaaaaatgaa aataaacttc
     3841 caaaatacaa gctctaaaaa ctacctctaa agttgtataa tgtttactag gaataaggtt
     3901 gtataacata tagtttaaat aatcattttt taaaattgca agttattgct ttcaaaatta
     3961 gatgtttgta tatcaaataa atgattaaga atttctaaat ttaatggctt tataatgcac
     4021 tccctttctt tgggataaaa taatgttgca cagatacttt ttctaaagat aacaatttta
     4081 tttttctatc aattccacca gcatacccag tcaaactccc atttgttcct acaacacgat
     4141 gacatggaac aataatagag attccattat gccctactgc accaccgatt gcttgtgctg
     4201 acattttaga tattcctttt tgttgagcaa ctttatatgc aatttctcca taagtgattg
     4261 ttttaccata tggtatttgt tgtaaaattt tccacactgt aatctgaaaa ggagtaccta
     4321 ccatatggag tgatggtgta aaattgggtt cttttcccga aaaataaata tctaaccatt
     4381 tctttgtctg ctcgaaaatt ggcaattctt tttcttgatg ttcttggtca agattataag
     4441 cataatactt ttgattctca aaccaaagac cagttaaccc aatattatct gatgctaaaa
     4501 gaatatttcc aattggacta tggtaatagc taatatattg catacaattc ctccttatat
     4561 tctgaaaact tcactcaaat cattagacac ttcttttcta tctaccatta tgtaaaaaca
     4621 tactaatata atttccttct aactacactt tcgttataat atctatcctc atatcatatt
     4681 agttttatat ttacctgcat ctgttcatcc ttttttccct ttccttttga tgaatataaa
     4741 attcatacat ttaagcttcc tactgtatta acttttatag aagtgtatat tataatttta
     4801 tcactaaata gaccaaaaaa atatcgatat aacatcaagt aatatcataa tactttcaaa
     4861 tgggaaaagt aagaataggc tgtaaaaaag aaaagtattt ttatgttaga aatccagtaa
     4921 aatcgttagt aaatagaata acagaaaacg aacaattcgc tatttatcat tttacacata
     4981 aatataattg gcgcaataaa gtgttttcta ttctacaacg attaataagg ttagaaaaaa
     5041 gtaaaaatga actgtatatg tatgaagcat tagtttctgt ttctttcctt tggcttatta
     5101 tgataaaaaa tattgttttg ccaccaataa aacacaaaaa tataattaat atccatatgc
     5161 aaaaaatttt acgttatatt gaaaaatact attgttttac attatattga agggtactat
     5221 tgtgaaaact taattttgga gtactatcaa gcagtgataa tatcaataaa tcagagtgtt
     5281 ccagaggatt tgaactaagt ttaaatacta taccttgtaa atattttact gagttttgtt
     5341 tactaaaagc aactcaattt ctaaaaggca caaatgagct aataagtgac attgctataa
     5401 aagttgtttt tcatcaaata atctatttta gtaaatgttt taaagaaaaa acgggatatt
     5461 ctccaaaagt gtacagaaat ataaaaaaag agatatgatt ttttatttct tttgattatc
     5521 caaagagtaa ataaaaaata ttgtaaaaaa cttactaaca acggcaatgc tttgaactat
     5581 ggtaatactt tgaacttttg attggacttt ttttgctaaa ataatatatt taaatagcaa
     5641 agtaatctgt ttaatcaata aaaaataaga gataaaagaa atcaactaaa acttttacct
     5701 cttattttga tagtttatta gagctaatat ttacactctt tttatttttg atacttcatt
     5761 aaaaatgatg cttattctct tttcattttt aatacttcat tagaacttat gcttattctc
     5821 ttttcatttt taatacttca ttagaactta tgcttattct cttttcactt ttaattcttc
     5881 attagaactt attcttactc tccattcact tttaattctt cattagaact tattcttact
     5941 ctccattcac ttttaattct tcattagaac ttattcttac tctccattca cttttaattc
     6001 ttcattaaaa atgatgctta ttctccattc acttttgata cttcattaaa aatgatgctt
     6061 attctctgtt cacttttaat tctttattaa aactgatgct tactctccat tcacttttaa
     6121 ttattcatta aaactgatgc ttgcactctg taaactcata aacatccatt aatgcttcac
     6181 caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata tctttcaaat
     6241 cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt tgttcagcag
     6301 ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa cttgtagtaa
     6361 agtttactcc attagaatct actggctgaa gaccatgtcc aaaattagca taattactac
     6421 caagtccagc agccttaagc tcttctggag tacaattgcc tattaattga tataacactg
     6481 aagaaattat ctcaacatga gccatttcct ctgtacctat atcagttaaa agtgcacgag
     6541 attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct gccaactcac
     6601 cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct gcttgtctta
     6661 tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac tcctccttaa
     6721 tatatattta ataataaatc taattttaaa gtttaataat tttatttata aaatgatttg
     6781 tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc atatccaaaa
     6841 ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa tttatcgcag
     6901 tatctgttgt atgaatcaag agctttctta tcccttggat tgttatctaa atataaattt
     6961 aattctatac aggcaaattg atattctgaa attctctcta aaagttctct tctatttaat
     7021 gacattagta catgttaccc ccttttttag ctctaactct atttttagga tttgcatata
     7081 gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa gtacctcttt
     7141 ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg tatggtctag
     7201 caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat tatcctcctt
     7261 aatttttaga attttaaaat agcattattt ttaaaaattg tatactggtt ctatattata
     7321 actggtagtt ttaataggtc tttaataaga ttaaatgatt aaataagtat ctttttttct
     7381 tattttacct ctaa
//
LOCUS       DAENCV010000163         7358 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAENCV010000163 DAENCV010000000
VERSION     DAENCV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.166"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            161..3340
                     /locus_tag="KQD82_003427"
     CDS             161..3340
                     /locus_tag="KQD82_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG4381870.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMKLENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3625..4389)
                     /locus_tag="KQD82_003428"
     CDS             complement(3625..4389)
                     /locus_tag="KQD82_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4381871.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4545..4778)
                     /locus_tag="KQD82_003429"
     CDS             complement(4545..4778)
                     /locus_tag="KQD82_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381872.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPSTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4852..6345)
                     /locus_tag="KQD82_003430"
     CDS             complement(4852..6345)
                     /locus_tag="KQD82_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG4381873.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6673..7215)
                     /locus_tag="KQD82_003431"
     CDS             complement(6673..7215)
                     /locus_tag="KQD82_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4381874.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2702 a         1065 c          816 g         2775 t
ORIGIN      
        1 tataagataa ttcaataaaa atctaatagt aaattgttat ttacacatat attgtgatag
       61 ataaaagcta ttcatatctg ccttaatttt agtcttaaat ttagagtgta aattgaatgc
      121 atttagatta taatatatac agtgaggtaa ggtgaataat atgaaaatag atatgatata
      181 taaactaata agaagaaata cagaaccatc aagatggtca agaggtgtaa aatattatac
      241 taatcaatta gttgattata tagacataga tattgatgac ctacaaaata acctgttgac
      301 atttcgtggt ggagtcaatt ctgaagataa ttatagcttt tattttaatt caataactat
      361 agatataaag cactttaaga taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa
      421 gggcaaaagt atttttatat gtaagcatat agttgctatt gtgataaaaa gcttaaaaca
      481 aataaaaaat aaaaaagatg ttcctcctat tctaatagat atattaggtg cagataagga
      541 taagctttct aatacaaatt ctaaaaccag cttagattta agcaagcaat tactttcttt
      601 gttagacaat actaaaagag aaagggttaa tttagatgta ttgataaatg tggattcaaa
      661 taaagcaatt gctgaattta aaataggaat tgataaaatg tacattttaa aaaacattac
      721 tgaatttgca tattcaaaac ttaatggata tgaattagat tttgggaaga aatttactta
      781 caaccccaac actcattatt ttgctgatga agatgagaag ttagtagata ttatagaaga
      841 atatgggaca aatctttcat acaacttttc ttcatcacat tcaaaaaaat ttatggagat
      901 aaatgcaagc tctttaaaac aatttttaaa tgctattaag tacaaggatt ttacccttat
      961 tttttctaaa gaggaatatc atccaaagat attaaatgat actctacctg taaggtttga
     1021 ttttaaaaat atagaagata aaatagtctt atattctaaa gattcactac ctgttccact
     1081 tactaaaaaa aatgatgtac ttttatatga tggcaatatc tatttattat cacataaaaa
     1141 agctcatgat tattcaaaaa tatatcaaat attatcaaag actaaagagt taaaatttga
     1201 taaagaggat tctaaggatg ttttaggatt gctagttcct agattaaaat ctatatctca
     1261 agaagttcat ttagatgata acataaaaaa taatataaca aaagatttta aatctgagtt
     1321 ctactttgat atgattgatg gcaacatatg ttgtgatgta aagtatatat atgatgatga
     1381 agataaaaaa tttgtgctcc ccaacataca aaaggaagcc actattgaaa ataaacttac
     1441 ttcttcacaa tttacaaagg aaaataatca ttatgtgttt aaaggaactg accatgacct
     1501 atttacattt ttagatttac aattagaatc tctaaaggaa tttggagata tatattattc
     1561 tgagaagttt aaattgaaaa aaatatataa cgcttcatca attcaagctt caatcaatca
     1621 gactaaccag aattacctag aatttaattt taatatatct gatataaatc caaaagagta
     1681 caaggatatt ttgaaagctt ttcaagaaaa acgtactttt tataaactta aagatggaaa
     1741 ctttatagat ttatcagaaa gagaaacaaa agattttttt gaattagttg aaaatctaga
     1801 tataatggat aaatcaaaaa ataataaaat tcataaaaat aaagctctat atataaatga
     1861 tgtaatacgt agtaaaaatt taaagtttat agatggtaaa aaagaactag acagtatatg
     1921 tgaaaaattt agaaattttg atagcataaa tttagaaatt cccacaaatt taaatgcaaa
     1981 ccttcgtgac tatcaaataa atggcctcaa ttggtttaaa gttttagatt attataaatt
     2041 tggtggtata ttagctgatg aaatgggtct tggtaaaaca atacagacta ttgcattttt
     2101 attatcatta tctaataaaa aaagtcttat tgttacaccg acatcactca tttataattg
     2161 gaaaaatgaa tttgaaaagt ttgctccaga tataaaagtt ttattgatac atggaaataa
     2221 aagagataga gaaaaatgtt ttatgaaatt agaaaatttt gatgtaatac ttacaacata
     2281 tggtactctt agaaatgatt tagacaaata tagtgaaata aagtttgatt attgtatatt
     2341 agacgaagct caaaatataa aaaatccagt agctttagta actgagtctg taaaaagtat
     2401 aaatgcagaa aataaatttg cactcactgg tacacctatg gaaaataatc tactagaatt
     2461 atggtctata tttgatttta taatgcctgg ctatctatat agtaaagcaa agtttcagga
     2521 attgtttata aataaagaag ataatgttaa aaacctgaaa aaattgataa aaccttttat
     2581 tcttagaaga agtaaaaaac aagttatgaa agaacttcca gataagattg aaaaaaactt
     2641 ttttgttgaa ttgaataaag agcagaaaaa gatatatagt gtatattcaa aagatataca
     2701 agataaaatg aaagataaaa atctaaagaa agataaaata gttatatttt cttatttaac
     2761 taaactaaga caactttgtt tagacccaag tatagtagta aaagattaca ataaaaaaag
     2821 ttcaaaaatt gaaacatgtt tagaaatact tagggatagt ataaacgaaa accacaaaat
     2881 attattgttt tcacaattta cttctgtgct taagaacata agtaaggaac ttgataagta
     2941 taaaattaaa taccattata tagatggtaa aactaatgcc aaagaaagac ttgaacttgt
     3001 agatgagttc aataatagta tggataaaaa agttttttta atatctttaa aagcaggcgg
     3061 aactggttta aatcttacat ctgctgacat ggtaattcac tttgacccat ggtggaatcc
     3121 atcagtagaa aatcaagcta gtgatagagc tcatagattt ggtcaaaaaa attcagtaca
     3181 agttataaaa ttgattgcta aagggactat agaagaaaaa ataataaaat tacaagaaag
     3241 taaaaaagag cttataaatc aatttataaa tggagaactt tctaatgaag gtgtattaaa
     3301 aagtctatct gatgaggaga taataaactt atttaactaa attatttaat cctacagctt
     3361 aactatataa atttgttcta acatttatat agttaagcta aaatatctga ataaataaaa
     3421 tttaatattg ttatttaatc ccaactttac aatctaatga tatctctttc tatacatatt
     3481 gcatatacaa atgtaataac ttgactatta gaatattttg ttttaacaat actatttcat
     3541 cataataatg aagtatatgt acactcactt ttgtaagttt tgcaatccat tttacagttt
     3601 tcattattgt aaattttggt taaattattc ctctaaaaat ttctttgcta catttgaaaa
     3661 atctaatcca ttcgataaaa ttgcaggatg accaccttta tcaaacaaat acattttacc
     3721 atttttaatc ttttcaagaa gtaatgaaaa agtattttca aaaaaatcac tagatattag
     3781 agatacaaac tcatcttctc tacttcctgt taaaagcacc tctggttgca tagtttctag
     3841 aggcttatga aaaaactttc ctatagtttt ataatgttca aaaatagctt ctgtatcatt
     3901 atctacaact ttttcccagt tttctccttg attataaata taaaaagctt ttgcaccatc
     3961 atcttgctta gatgcttcac gctctatttt cacattttgt acaaatgact ctagaggaac
     4021 ttctccttca aaactatctg caattacctt attcactaaa tcaggtctct caagtgcaac
     4081 atttaatgca acaagagcgc ctccactact accaattatg tttacttttt tatagttcat
     4141 tgcttctaaa aaacatatca cttgcatagc ctcatcaaac cataagtcag cagaaaattt
     4201 atcgaccatt tgagattttc catgacctac aaaatcaatt aatattactt tatattcatc
     4261 tttataaaaa tcgattatat cattaaacat cttggaagag gctgtatttc catgtaaaaa
     4321 aagtaatggt gtaccatccc caatttcatt atagtagcac tgtttgtcgt tatagttaaa
     4381 atatgccata tatttccccc ctttaatatc cctataggta tatatttatt acttatattt
     4441 aaaatgacta ttccattagc cacttaaaaa tagtataact tagaatgtag ataaaatcaa
     4501 cattccttgc taatttatct attgtttgtc taatttatta taatttaact tctgtaaaat
     4561 ttcttttttg tacgaaaata ttccttccat ttacaacaat cattcaaatg aagtataaca
     4621 tgacgagtag aaggcataag caaaattcca gcaatatctt tattccccca aaagtccaac
     4681 aaccaaatag aatcttcatg ctttattact cctccagtca ccaaaatcct ttctaagtca
     4741 attggagaga cctttcgttt catatcttca gctttcaaat ccttaacaac ttctattgtg
     4801 ttttttgcaa cttcatttcg ataacaaagc aattcatcaa tattgactaa attatcgtat
     4861 atctcttttc actttaacca tttttttctt tataggtatc tccttatatt tttttaatat
     4921 caaatctccc ttataattta atatatctac atatgaacat aaatcttgaa cttcaattac
     4981 acctatatca tcattagtta taccttttat attactaaat gcaccaacta tatctaaaac
     5041 tcttattttc ttcttcttac cagcatttaa atataattta acaacttcac tttctatatt
     5101 tagttttata tctttacttt tgtaagaatt tttatcaact ttttttctct ttgatacttt
     5161 ttttaggatt ccttttgaat attcttcaaa caaaactttg ccatgtgtaa ttctactttt
     5221 ttctatatct tttaattcat ttattttata tcctatatac gtttctatat tctctatata
     5281 tttttcatcc ttttcagtta ctatagtaat tgcctttcca ctatttcctt ttctcccagt
     5341 tctacctatt ctatgtatat agttttcttt atcttgtggc acatcataat ttattactaa
     5401 agaaatatca tcaatatgta ttcctctgga cgctacatca ctactaacta atatattaaa
     5461 tttttggttt ttaaaatcct taattacaaa aattcttctt tcttgtgata aatctgcatg
     5521 taactctctt attaaaaaac cctctttact catcttgtta tataatttag atactttctc
     5581 tttggtatta caaaatatta tcacactctt tggattttcg ctgtatatta attcttttaa
     5641 aaggatgtac ttctctcttc catttgcctt gattattttg tcatctattt gtcttgtttt
     5701 ttgattagta tcgctctcat tttcttctat atttataaca ctgtaatcta gcatatattt
     5761 ttcacatata tattttatct cttcatctat tgtagctgaa aaaagactta ctattttctc
     5821 cttaggtaaa tttaataata ttttttccat ttgttcaaca aaacctttat taaacatttt
     5881 atctgcctca tctattacta aatactttac attttccaac ttaatagaac ctctatttat
     5941 atgatcaagt attctgcctg gagtagctac aacaatatga accctttgtt tcaattcagc
     6001 tatttggtct tttatagatt gtttgccaaa tatagcacta catctcacct tctttagtct
     6061 ccctatatct gatatctcat cttttacttg caaggctaat tctcttgttg gaaccactat
     6121 aagagcttga atattattgt aatctacatt tatattctcg cataaaggta ttgcaaaact
     6181 tgctgtcttt ccacttcctg tttttgacct aactactaag ttttgacctt ttaataattt
     6241 tggaataact tctctttgta ctctagaagg tatattatat cctaaactct tcaatgactt
     6301 aagtatcttt tcatttaact taaatttttc aaatgtatat ttcatatatt accaccttat
     6361 tatatttatc ttatctatat atttttctac attatattat tataatacaa ggttctacta
     6421 tttttatatt atttattaga ctgaagataa aaatatatac tctataaacc tatagtttag
     6481 tagcaaatta attaaatttt tatatttaaa tacctcctta aatatactga taaatcaata
     6541 ttttcaagga ggtatctctt gtcaataatc aaactaatct atcttttgaa agtttaaatt
     6601 atactaatac attgtatttt tatttatatc taatattaaa aggtattaat caatacctaa
     6661 atcaccataa tctcacatat ttatatcttt attcaatata ccttctgtaa tcaatcgtgc
     6721 tatataatca taacctgatt catttgcttt gttacaatca ttcacattat cacagaaaaa
     6781 actttcaatc tgtattgcaa ctggatttgt atttctcaat atatataaat tttctctttt
     6841 tttagctcct ctattttcaa ataattctcc aagtctatta gatactcttt tagcatattc
     6901 taatccttca gtgccatagt aaagcacttc tgtaccacat tgtgttccat cagctttatt
     6961 taaatgtagc tctactaata aatcgtattt accactgttt acttttggaa ctctatagaa
     7021 aaattcttct ctttctgaca taaagtgcct ttctggacaa attattacat ctactgtatt
     7081 accttgactt ataagcatct cagctaacat aggagctaat tttttattat aattgtattc
     7141 atcaactata ccatttacac ctgtactttt accacctttt agtatactat gacctactgt
     7201 tatgcatatt ttcatttact tttttcacct tcttttaagc caatttttat ataatagtaa
     7261 tataatttta aagttattac taataataat atattaattg aagaaggtaa aagttctccg
     7321 tgattatcat ttttttaaca tatttgtctt attttatc
//
LOCUS       DAENCV010000164         7233 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAENCV010000164 DAENCV010000000
VERSION     DAENCV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.167"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..476)
                     /locus_tag="KQD82_003432"
     CDS             complement(<1..476)
                     /locus_tag="KQD82_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG4381875.1"
                     /translation="MKIKKLLIIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNA"
     gene            667..1488
                     /locus_tag="KQD82_003433"
     CDS             667..1488
                     /locus_tag="KQD82_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG4381876.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            1613..3130
                     /locus_tag="KQD82_003434"
     CDS             1613..3130
                     /locus_tag="KQD82_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG4381877.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLNTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            3165..3437
                     /locus_tag="KQD82_003435"
     CDS             3165..3437
                     /locus_tag="KQD82_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4381878.1"
                     /translation="MSLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQ
                     LRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(3534..5198)
                     /locus_tag="KQD82_003436"
     CDS             complement(3534..5198)
                     /locus_tag="KQD82_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG4381879.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQILIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(5270..6517)
                     /locus_tag="KQD82_003437"
     CDS             complement(5270..6517)
                     /locus_tag="KQD82_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG4381880.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(6504..7178)
                     /locus_tag="KQD82_003438"
     CDS             complement(6504..7178)
                     /locus_tag="KQD82_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG4381881.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
BASE COUNT         2533 a         1132 c          917 g         2651 t
ORIGIN      
        1 gcattagtca atatgacttt atttactttg ttaattgagt ttatttcttt tgcgacatta
       61 tagcttgtct taattctatc actaccttga agtcttttaa tctctattcc tttgcctttt
      121 agaacagttt cagtatattt atcaatagag ttttctcctc caattatata tacttttttt
      181 gccttttcta atcttttaag tgtagcattt ggtatgttat ttttcttagt caaaagaatt
      241 ggagcgttta tagctcctgc aagtccactt gcacttaatc catcagccat tgtactgtct
      301 gcattaatta atatagctgt agtatagttt tgtttgtctg caattatacc agcagtttcg
      361 tatttatctg cacctttaat tgattcaatt ttatcaagag catttgcaga tatagggcaa
      421 ctagcaataa atatagataa agataaccct attattaata attttttaat tttcataata
      481 tctccttctc ttaaatattt tcacctaaat tatataatac ttttcaggaa aattttacca
      541 taaatttaaa aagttattat tacttaatat aatattaatg aaaaatatca ctcttaaatt
      601 aatgactttg gtagaatatg gtgataaaat aaaattaatg agagacaata taagggggat
      661 tatattatga taatcagtaa aaaaataaca gcaatattag tatcattttt aattatgata
      721 tcaatggttg gatgtgctcc aaaagaaagt gctacagaag tagctaaaaa gtaccttgat
      781 agtatagaaa tcaaagacta cgaagtagca tataatttat taagtaaaga cagtaaggaa
      841 agtgtcagtc tagaaaaatt tgaagaatat caagatactt tatttaaact tcaaacccta
      901 aaagattata aacttggtga agaaaataaa gtagagaaat acaaatattc taataaagag
      961 tacacaaatc ttgttaaaat aaaagaaact gatactataa agtatatcac tgaaaataaa
     1021 gaagatactg caaattttga tagatatttt ataatcgaag ataatgaata taaattatta
     1081 tggcataaag actttaaaag agctcttagc tctaattatg tcaaaatcgc taggtcagaa
     1141 acagaaaaag atgaagataa ggattataat aaagctgcta ttgatatcaa aaaagctata
     1201 gaaattgatg ataaaaatcc acttgcatat tatacacaat catatattta tatagagtta
     1261 caacgatatg atgaggcttt aaagtcaatt gataaatact tagaatatct agataaaaat
     1321 aatgaagctt ataaaataga cgctagtgat gcttacaatt taagaggtgt tatttttatg
     1381 agtaaatcag aatttaataa agctagagaa tcttacaata aggcgcttga acttaaccca
     1441 gataatgaat atgcaaaaac taatctaaat gatattaaag gattataagg cattaatatt
     1501 atagaaaata agtatgttga aagttaaatt aacttttgac atgcttattt tctatatttt
     1561 aaactctatt gatttactat ataatagtgc ataaggagtg tgattactta ttatgagtgt
     1621 tgccatatac ttaagaaaat cccgtgctga tgaagaggca gaaaaacaag gcgaatttga
     1681 aactttaagt agacataaat caaccctact aaaactagcc aaagaacaaa acttagatgt
     1741 aattgagata aaagaagaat tagtttctgg tgaaagtata atacatagac ccaaaatgct
     1801 tgaacttcta aaagaagttg aagaaaataa atatgatgca gtcttagtaa tggacttaga
     1861 cagacttggt cgtggagaca tgaaagacca aggtatcata ttagaaacat tcaaagaaag
     1921 taagaccaaa attatcacac ctagaaaaac atacgaccta acagatgaat ttgatgagga
     1981 gtattcagaa tttgaagcat ttatggcaag aaaagaatta aaacttatat caagaaggat
     2041 gcaaagaggt agaataaaat cagttgaaga agggaatttc ataggaacct ctgctccatt
     2101 tggatatgat gctgtaacaa ctggtagaaa agaaagaata cttgtaccaa ataaagatgc
     2161 tgatgtagtt aggactatct ttgacttata tattaatgaa gatatgggtt gtagtaaaat
     2221 atcaaaatac ctaaataact taggcattaa aactgctaca ggtgctaatt ggtacaattc
     2281 tgcaattaca aatataataa aaaataaggt atattgtggc tacattcagt ggcaaaaaaa
     2341 ggactacaaa aaatctaaaa atccaaataa aataaaaaca gttaaactaa gacctaaaga
     2401 tgaatggatt gaggcaaaag gaaagcatga acctttaata agtgaaataa catggaaaaa
     2461 agctcaaaat atactcaaaa aaaatggtca tgtcagctat ggtaatcaaa taaaaaatcc
     2521 acttgcagga atagtgattt gcaaaaattg tggtagacca ttagtatata gaccatatgc
     2581 agaccatgac tatataattt gttatcatcc tggttgcaat aaaagttctc gatttgaatt
     2641 tattgaagct gccattttaa aatctttgga agacactgta aaaaaatatc aattaaaagc
     2701 atctgatata gacttagata aaaataataa agatagtaat atagaatttc aaaaacgagt
     2761 cttaaagggc ttagaaactg aattaaaaga attaggcaag caaaaaaata agttatatga
     2821 tttactagaa cgaggaatat atgatgaaga tacatttatt gaaaggtcca acaatataag
     2881 ttcaagaact gaagaaatta aagattcaat taagacagta aaaaatagat tgaatactgt
     2941 taaaaaagac aattctaaaa taatagaaga tattaaaact gtgttatcac tttaccatga
     3001 ttcagattcc cttggaaaaa ataaacttct aaaatctgta attgataaag cagtttatta
     3061 taagtctaaa gaacaaaaac tagattcttt tgaattaatg gtccatttga aattacatga
     3121 agaccagtaa taccaataac atataaagag tagtatgtaa tgaaataagt cttttagata
     3181 tacttggtac agaggctaac tatgttcttg atgaagttga actaaaagtt caagttggaa
     3241 aactttacga acaactaaac aaaattctta ctcctagaga aagagaaatt gtccagttaa
     3301 gatatgggtt aactccttat ggctataaga cacaaagaga aattgcacaa aaactcgata
     3361 tatcaaggtc atatgtatct agaatcgaaa aaaaagctct taaaaaacta gaaaaagagc
     3421 ttgttcaaga aagttaatac tatattttaa aaggatatat cgtacatcat tagacaaaat
     3481 tttctatcta aaatcaaagt tacgatatat ccttaaattt tatagtacat aatttatttt
     3541 atactttcac atatttcctt aaatataggt gttgcagatt tatttccttt ttgagttcct
     3601 tctactaaca cagttattac atacttaggt ctttcttcag gataaaatcc tgttatccat
     3661 ccgtggtcta tagctttttt atttaaacta ctttgagctg taccagtttt aactccacaa
     3721 ccaccttcaa tatcttttag gtctttagct gtaccagtcc tagatacaga cttcatatat
     3781 tctttaactt gtgtacacac ataaggagaa ataagttctt ctttttttga agacttataa
     3841 gtttttattg tattcatatt attgtccaca agacttttat ataagtatag tggtttgaat
     3901 gttccattat tagctattat ttgagtcatt tgatttattt gaagtggtgt aaattctata
     3961 ttttcttgac ctatagctaa atttcttatt gatatgtttt ttggagcttc tctaattttt
     4021 tcttcatcaa gaccaatatc aaccttttca aataaatgta atttttcagc agatttgagt
     4081 atcttttctt ttccaagttt catagctata tctaaaaatg caggattgca tgaattagaa
     4141 aaagcttgtc ttaagttttg aaaaccatga ccatctcttt tattacatcg cagtatttca
     4201 ttagtattcc caactttagt cttacctgta cagttgtaag tatagttctc atctataaca
     4261 ccattttcaa gtgcagaaaa taaaactacc atcttaaata cagaccctgg tggatatgtc
     4321 gcttttataa ctctattttc aaattctcca ttttttcctt ttaaactttt tgatatgtca
     4381 ttttggtcaa aattaggtct agagcacatt gcaagaattt cacctgtaga tgcttccgag
     4441 attattgctg ctgtaggatt ttcttcttta tttagtattt gttctaattt tttttgaatg
     4501 ttataatcaa tagtagtttt tagatgtcta tctttatcct tattatcaac agtttttaca
     4561 cttcccttta gtatgtttag agatttttca tttcctgcat ctccagcttt aaaagcactt
     4621 atatactttt catttgaatt tctaagtaca ctatccatac tcttttcaat tcctgattga
     4681 cctgatttat catcttcttt tatatatcct attgtatgtg ataataaacc atcttttgcg
     4741 tacctcatag ttttttcttc taccattatt ccatttttct caagttcttt tttcatactc
     4801 ttatcgatat ttttagtctg aatctgtatt atagggcgtg tcaattgctc ttgtacagct
     4861 ttatatatat ctaaatcatt catcttagta gctttcttaa taagttccaa aattttatag
     4921 ttattattta atttttcttt ttcaacaatt aatatctgag atttacttgt atctgtaagc
     4981 ttcttgttgt ttctatcata gattattcct cttccactat ttaactcgac tttctcaacg
     5041 ctttgtgatt caacactttg tttgtattta tcacctttta atacttgtat atcaactagc
     5101 ctataaatta aaacactata aattattaaa attatagtaa atatacacca gcttctcttt
     5161 cccacttttt tcaaaaatgg agtcttcttt tttgacatga aatcacccct tagataattt
     5221 cattattgac aatttctaag gggctaatac tatttttatc aattttttat tattcttcaa
     5281 ccttttcttc tataactttt ctaagcatat aatctttacc aacttttact gatagtttta
     5341 acttcactat ttgtctagga tgaggagctg attctatagc ttctccttct tcattgtaca
     5401 tttcttcaat tttagtaaac atagtttctt tatatggtcc cataacttct atttcatcac
     5461 caacacacat tttatttctt tgctcaacaa caataagacc atcttcatct tctgtttctc
     5521 taactatacc tataaaatca tagtttctaa catatgatgc tgattgataa ttttggtctt
     5581 ctgttgttgg tttattcaag taaaaacctg atgtaaaatg tctatgacta ccttttttta
     5641 gttcttccat ccacattgga ttgaacttcc agttttctgg gtctctgtaa aattcatcta
     5701 ttgccattct gtatgctctt acaacagtag ctacatagta tgcagtcttc attctacctt
     5761 ctatttttaa acttgttata ccagatttga ttaactctgg tatatactct atcatacata
     5821 aatcttttga gttaaagaag aatgtacccc tttcatcttc gtatattgga aaatactcac
     5881 caggtctttt ttcttccacc aaatgatatt gccatctaca agattgagca caagaacctt
     5941 tgtttgcatc tcttccagtc atatagttgc ttattaaaca tctgccagaa taagaaatac
     6001 acatagcacc atgcataaac gcttctatat ccatatctaa aggtgttttt gctcttatct
     6061 cagatatttc atcaaaagat aattctcttg ccactacaac tctttttatt ccatgctcgt
     6121 accagaagtt tgcacttaga taattggttg tatttgcttg tgtacttaaa tgtatttcca
     6181 tattcggaat tactttttta atgacactaa gcactcctgg gtcagctatt ataactgcat
     6241 ctattcctat ctcttctagt tccaacaaat agtcttctaa ttgtagaaaa tcttcattat
     6301 gaggtattat atttactgtt acgaatactt tcttgcctct ttcatgtgca aaagctactc
     6361 cctctgccat atcttcctta ctaaaattct tcgctgcaga cctcattcca aaaatttctc
     6421 cacctatata aacggcatct gctccatatg ttatagctat tttcaatctc tccaaatcac
     6481 ctgctggagc taaaagttct actttattta attccatcta catcacctct tttactttct
     6541 ttataactta gtgctacacc atcacctatt ggtaccaatg atgtgcttaa atagtcacaa
     6601 ttacatatat aatctaaata atttctcatt cttttaacta tagtcttttt tcttcttaca
     6661 acaaaatcat catgagcaac cataccttta tacaaaatat tatcagatac taaaagtccc
     6721 ccatctttta atttatctat aactaaatca aaaaatagct tatattgacc tttggctgca
     6781 tctataaata taaggtcaaa ttcaccttgg acttgactaa gtttttcttc tgcatctcct
     6841 tctagtatag ttatgttatt gtcaaatcca gctaatttta tgttttcttt tgctttttct
     6901 atcatttttt catttctctc aacagttatt atatctgcat cttttcctat aatagttgca
     6961 aaaaaaatag aagaataacc aattgcacat cctacttcta aaattctctt tggtttttga
     7021 acctttaaca agacttttaa taaatctgaa acttctttat gtattatagg aacactattt
     7081 tcctctgcat aaatttctaa atcttttaaa aatccttctt tttcttttaa tgtcactctt
     7141 atataatctt caaccaaatc attaactata ttactcataa aaacactccc taaaataata
     7201 aatattcaag ttttatatta atttattatt ttt
//
LOCUS       DAENCV010000165         7123 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.168, whole genome shotgun sequence.
ACCESSION   DAENCV010000165 DAENCV010000000
VERSION     DAENCV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.168"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            414..923
                     /locus_tag="KQD82_003439"
     CDS             414..923
                     /locus_tag="KQD82_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG4381882.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            951..1145
                     /gene="rpmI"
                     /locus_tag="KQD82_003440"
     CDS             951..1145
                     /gene="rpmI"
                     /locus_tag="KQD82_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG4381883.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1201..1557
                     /gene="rplT"
                     /locus_tag="KQD82_003441"
     CDS             1201..1557
                     /gene="rplT"
                     /locus_tag="KQD82_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG4381884.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1867..2412
                     /locus_tag="KQD82_003442"
     CDS             1867..2412
                     /locus_tag="KQD82_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381885.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2399..2572
                     /locus_tag="KQD82_003443"
     CDS             2399..2572
                     /locus_tag="KQD82_003443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381886.1"
                     /translation="MKGNKSDLHGVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2847..4772
                     /locus_tag="KQD82_003444"
     CDS             2847..4772
                     /locus_tag="KQD82_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4381887.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYINEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDELTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5173..7077
                     /locus_tag="KQD82_003445"
     CDS             5173..7077
                     /locus_tag="KQD82_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4381888.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         2999 a          712 c         1348 g         2064 t
ORIGIN      
        1 aatacaatat agaatttgtt gggtagtata gtgttatatt agaatacaat ataggatttg
       61 ttatgtagaa tatatagagt catgtagaat atacatatat aaaaggattt tgggtataaa
      121 tttaaaaatt aatagcaaaa tgagtagtat agacatataa tgagtagtag gaagtaatta
      181 aatttttcct aaacaaaaaa aatagttgca aataatttga ttaagtgata tagtaaataa
      241 ggtaagaata aaacaaagaa gaagtccgct tctcacctta cgccggaggt tagtaaggta
      301 aatataagtt aaagcttaat tttaatttaa gtttgtttaa atttggtaac ttatattgcg
      361 gatgattacg tcatccgctt tttattttat aaaaattttg gaggtgtcct acaattagca
      421 aagaattagc aatcaatgaa caaataaaag ataaggaaat aagagtcctt tcaccaacag
      481 gtgaacaact tggagtaatg cctactaaag aagctcaagc aatggctaat agcaaaaact
      541 tagatttggt tcaaatctcg ccgaatgcaa atccaccagt atgtaagata atggattatg
      601 gaaagtttag atatgagcaa gcgagaaaag agaaagaagc taagaaaaaa caaaaaacta
      661 tagttgtaaa agaagtaaga cttagaccag gtattgaaca aaatgattta aatacgaaag
      721 ctaataatgc tataaagttc cttaaaaaag gggataaagt caaagttgaa cttagattta
      781 gaggaagaga attaggacat aaagacatag gtaaagaagt tatgcttaag tttttagata
      841 taataaaaga gtttggagaa ccaacaaaag cccctgcatt tgagggtaat aatatggttg
      901 taattataga tccaaaaaaa tagatgatta actgagagga ggaatttgtc atgcctaaaa
      961 tgaaaactca tagaggtgca gcaaagagat taaagaaaac aggaactgga aagttaaaga
     1021 gagctaaagc ttttaaaaaa catatattaa ctaaaaaatc tgcaaaaact aaaatgaact
     1081 taagaaaatc aactttagta agtgatggag atgctaagag aatagcacaa ttattaccat
     1141 actaagatac agattgtaaa taataactat agaagaggga tataaaagga ggtcataacg
     1201 atggcaagag ttaaaaaagc tatgaacgct cgtaaaaagc ataagaaaat attaaaactt
     1261 gcaaaaggat tcagaggatc tagaagcaaa ttatatagac cagcaaatac attcgtaatg
     1321 aaagcattaa aaaatgctta tataggaaga aagttaaaga agagagactt cagaaaactt
     1381 tggatacaaa gaataaatgc agctgctaga atgaatggaa tatcttattc aagattaatg
     1441 aatggattaa aattatctgg tgttgaagtt aacagaaaaa tgttatctga aatggctata
     1501 caagacccag aaggatttgc aaaattagca gaagttgcta aagcaaaatt agcttagtat
     1561 tgaatcatga gatgtaaagt ctttgagtta tgtaaataat tcaaggactt ttttattttt
     1621 tgaaaaatac ttattagtgt catgtacaac caattttata atgataaata aaataagcaa
     1681 tacaatagaa ttttatgaga tttattataa gaattgttac aattatttat gatagctata
     1741 aaaagtcata gtaaaaatga tattgatgta tgttgatata tgtataaagc tttcaatcaa
     1801 tcttgaatta atattatcaa catgataata ttatcttatt aataatataa ataaggggga
     1861 gataatatga atgaaaaact aatggattgt tttgtaaacc caattaaatg caagttattg
     1921 attgaaattt tttcaaataa tcgaactaca gctaaacaat taggagaaaa gtttcaagat
     1981 atatcaaagg caacacttta tcgttacctt aataaaatgt tgaaagatga tattataaaa
     2041 gtggttgaag aaaataaaat aaggggaact gttgaaaaag tatatgctat gaagttagat
     2101 atagctgatg atgtgaacaa aattatagat gagaactctg gagaagaata tgctcaactg
     2161 tttactcaat atatgatggg atttttaaga gaattttatg aatacgctga aagaaaggaa
     2221 aaggatatcc taaaagatgg ttcaggattt tctgtctgtc cagtatatgc tagtattgat
     2281 gagatagttg aagtgagtaa aaaaattgct agtatactag aacctctaag aaataactca
     2341 catgaagaag gtagaagaat gcattctata ggagtgataa ttactccacc taaagaggat
     2401 gaagggaaat aaatctgatt tacatggggt agagccaatt tatacagaga atcgttttat
     2461 gatttacata tgtatccaga gcaaagtatc tctgcttgga ttgtacttca ttgtaccatt
     2521 tcatccaatg acttggagtg gtacaatgaa gttttttaat ttttcatatt aatgctaaag
     2581 aaatgaactt tagaccacat atgcaattag gttgcattat acaattagac cacatataca
     2641 aaataaaaat cagtattttt atagcagata aattgactgt tattcttctt aaaaatcatc
     2701 tattagttat tgttagcata ttgaaagaaa ttatagtaat ctattgaata aatgaatatt
     2761 gcccataatc atataaactg ctatagttta aaataaagca ggacgttaaa ttttatagta
     2821 atctaaaaat ttaagagggg gattttatga aaataaaatt tgttattatg ccattggtga
     2881 tatttttaat ggtattattt gtggggtgtc aaagtacaaa agatacagaa gttaaagcaa
     2941 aagatacaga aattaaaata ttagggacag gtgatttaca ttcaattttg acagactcta
     3001 tggtttcata tataaatgag gaaagggaaa agaataaaaa tctgcttatg gtagatgcgg
     3061 gagatttcta tggtacacaa agtagagaaa tgtgggaatg gtcttctgga aaaaagttaa
     3121 ttaatattaa aagaaatggt agagcagagt atgatgacat aattaagagt agtaaagatg
     3181 aagtacctat agttaaagat atggcaaagt tgaaatatga tgctgtagtt ttgggagata
     3241 atgaatttat ttcaaatgat aagcaaagtc ttgataaact ggtttctgat tttaaaaata
     3301 ataatatgcc attggtatca gcaaatatat atgaacaatc tggagaaaat tatgtacaac
     3361 catatgtaat gaaaaatatt aagacagatg agggcaatgt aaaagttgga atactaggtc
     3421 ttactataaa agaagtaggg gaaacccttg gtcttgagga ttttgaaaag gataaaaaag
     3481 ctagagagct tggagaacaa gctgattaca aaggaaaatt atatgcaaat gatttagtag
     3541 aagatgcaaa gaaatggata aaagttatgg aaaaagaaag tccagacata atagttgcag
     3601 ttgtacactc aggggaagaa cctaaaacag atagaaatcc aggaaataga ataaaagagt
     3661 tagcaactac agtggatgga atagatgcaa ttgtagctgg tcatactcat gaaaaaatag
     3721 atgaacacaa atataaaaat aaatctggag atgaagtaat tgttacacaa ccaggttggc
     3781 atggagatag agtatcagag attaatttca aattaaataa agagaatgga aagtgggaca
     3841 taaaggaaaa gtctagtaaa ttaaaaataa ttgataaaga agtaagtata gttgctactg
     3901 cagatttaca ttcacatttc tctgatgaat taacagttga ccttgttaat gaacgtagta
     3961 acccaattga acctgttgta gtagatgcag gcgacttctt agaccctcaa acagatgaaa
     4021 tgactcaatg gtataaagag tggaaaagta taagagataa taatagtgat aaaacgatta
     4081 gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta gtacttggaa
     4141 atcatgaatt tgtatctgaa aaagatgaat tttggtcaga tgaacgcctt ttagatgctg
     4201 ttatagagga ttttgaacag gtggcaattt ctgtgttatc ggcaaatata tataggcaat
     4261 ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt ggagacgagt gaaggtaatg
     4321 taaaagtagg aatattggga cttactataa aagaagtagg taaaggtctt aaaaatgtaa
     4381 acttacaaga gcaatttcaa tataaggata aattgtatgc aaatgattta gtggaagatg
     4441 caaagaaatg ggtaaaagtt atgaaaaaga aaaaaccaga tataatagtt gcagttgtac
     4501 attcaggaga agaaccaaaa aatccaaaac atccaggaaa tagaataaaa gaactagcaa
     4561 ctacagtaga tggtattgat gcaattgttg ctagtcatac tcatgaaaaa atagatgagc
     4621 atgaatataa aaataagtct ggagaaaaag ttatagtgac acaaccaggt gagcatggta
     4681 aatattattc taaaattacc tttaaagtaa ataaagaaaa gggttcgtgg actattaatg
     4741 ataaattcag taaaacaatt gaggttgaat aactttataa aatttataga taaagtaaat
     4801 tatctgatat tgacatgata tataagataa agtttctaat attggtataa tgcataatgt
     4861 gaaatatttt aataataaga gaataaacaa gacaaataaa aatattttaa catgtaagat
     4921 atagtatgaa ctattatact aatgcttact ttataatttg aacatgttag aataagtctt
     4981 atttgaaaat tttagtgtat tagagtaagt attattttac tagtaaaaaa tattgagaac
     5041 tatatgtaga tttgcttaaa aaggaattta catatagttc ttttcgtata ataaatagta
     5101 caatcttata tggtaaagat aaagtgtgat attaaatttt attatcatcc aaaatctaag
     5161 gaggagtctc ttatgaaaat aaaatttatt atagtagcat caataatatt tttaatagta
     5221 ttacttgtgg ggtgtcaaag cactaaagat acagaaatca atgtactcgc cacaagtgat
     5281 ttacattcaa ttattccaga taatttaatt tcctatgtag aagaggagag aaaatatgat
     5341 gagaatctac ttatggtaga tgcaggagat ttttttgaca tgcaaagtgg tgagatgaat
     5401 aagtggttta ctggtcaaaa attagtaaga tttaaagatg ggataccaga gtacaaaaaa
     5461 atagctgaac cacgtgaagg agaagttcca ttggctaaaa aaatggcaaa gctgaagtat
     5521 gatgcagtta catttgggaa tcatgaattt gtatctaatg acaagcagag ccttgataaa
     5581 ttggtttctg attttaaaaa taacaatata ccattagtat cagcaaacgt atatgaacaa
     5641 tctggagaaa actacgtaaa gccatatgta atgaaaaata taaatactga caagggaact
     5701 gtaaaggttg gtatacttgg tctgactata aaagaagtag gggaacgttc aaggtttaaa
     5761 agttttgaac aagataaaaa ggctagagaa cttggggaac aacctcaata caaagggaaa
     5821 ttatatgcaa atgatttagt agaagatgca aataagtggg taaatgttat ggaaaaaaaa
     5881 gaaaagccag atataatagt tgccattgta cattcaggtg aagagccaaa aaatcctaaa
     5941 aatccaggaa atagaataaa agaactagca acaactgtag agggaataga tgctattgta
     6001 gcaggtcata cacatgaaaa aatagaacag catacatata aaaataattc tggtgaagaa
     6061 gtaatagtaa cacagcctgg agctcatggt aatagtatat caaagattaa ttttaagttg
     6121 gataagaaga atgataagtg gtttataaaa gacaaatata gtgaattaaa ggtttttgga
     6181 aaggaagtaa atataattac aacttctggt ctgaatccaa aattttcaga tgaaatggca
     6241 gttaatttgt ttaatgaaag agatagagat gttcaaccta tctttataga tactggagat
     6301 tttttaaata ctaatacaaa agaaatgact aagtgggcta aagagtggca atacaatgaa
     6361 aataaaggca tctataaaaa aataaatgaa tggccaattg tatatcttgg ttatgatgct
     6421 gtagtacttg gtagccatga atttacagag gataaagaga cttttgagga aaaaaaatct
     6481 acattggatt atattataga aaactttgaa gaaatgaaga taccagtatt atcagctaat
     6541 atatatgatg aatctggaga aaattatgta aaaccttata taattcataa tgtagaaact
     6601 agggaaggaa atataaaggt tggagtatta ggtcttacta ctactgataa ggatgaagaa
     6661 gacttaagta actcaagctt aagcaaagac tcaaagactt cgaaaattcc tgaagaaaca
     6721 ggaaagttat atactaataa tctagtaaaa gatgcaagcg aatgggtaaa agttatgcaa
     6781 aaggaaagtc ctgatgtgat tgttgcagtt gtacattcag ataataatag tactctaaaa
     6841 aatccaagta gtgaagtcaa aaaacttgca atgtcagtag atggaataga tgctattgtt
     6901 gtaggtcata cgaaaaataa ggtagaagaa catatttaca aaaataagtc tggggatgag
     6961 gttatagtaa cacaatctgg agaagattgt tattcaaaaa taagtcttga actaagaaaa
     7021 gaaaatggca agtggaatat cataaacaaa tatagtaaag caataaaaat gaagtgaatt
     7081 tattgatata taaaccatta ttaacactga tttttataat ata
//
LOCUS       DAENCV010000166         7018 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAENCV010000166 DAENCV010000000
VERSION     DAENCV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.169"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(147..998)
                     /locus_tag="KQD82_003446"
     CDS             complement(147..998)
                     /locus_tag="KQD82_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG4381889.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1047..2216)
                     /locus_tag="KQD82_003447"
     CDS             complement(1047..2216)
                     /locus_tag="KQD82_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG4381890.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2687..3604
                     /locus_tag="KQD82_003448"
     CDS             2687..3604
                     /locus_tag="KQD82_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG4381891.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3733..4662)
                     /gene="pfkB"
                     /locus_tag="KQD82_003449"
     CDS             complement(3733..4662)
                     /gene="pfkB"
                     /locus_tag="KQD82_003449"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG4381892.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            4929..5654
                     /locus_tag="KQD82_003450"
     CDS             4929..5654
                     /locus_tag="KQD82_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4381893.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5737..6891
                     /gene="nagA"
                     /locus_tag="KQD82_003451"
     CDS             5737..6891
                     /gene="nagA"
                     /locus_tag="KQD82_003451"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG4381894.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKNPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICMYKVNK"
BASE COUNT         2474 a         1061 c          909 g         2574 t
ORIGIN      
        1 attatatatg taatacttta ccaataaaag ctgatatttt tcatcaatta aatgaaaaaa
       61 atacaccatt tacatataat taatataaat ttttcatata acaaatatcc taaacttatc
      121 taaaataaat ttaggatatt ttatttttat actctattta cagacccaca cactttaatc
      181 ttttctttca caaaatcctt tactgattga agccctgcta atccatactt tttagggtct
      241 ataacctcag aatcttcatc taaaactttt ttaacccctt tactatatgc aattcttaat
      301 tcagttgcat agttaacttt tgcaatacct ctttttatag cttcttttat aatatcatca
      361 tgtattcctg aacctccatg caatactaaa ggtattgaaa ctacattttt aatttctgat
      421 aacctatcta aatctatttt aggttcaccc ttatacaatc catgtgcagt acctatagca
      481 atagccaatg aagatattcc tgttctatcg acaaactctt ttgcctcttt tggatttgta
      541 taatcattac tattcccgcc atctaaatca tcttcttttc caccaacttt cccaagttct
      601 gcttccactg gtataagaga aggactacat gcttttacta ctgatttaga tatagatata
      661 ttattttcaa agctttcatg agaaccatct atcattattg atgtatatcc acttcttaaa
      721 gctttcattg caaggtcaaa acttgaacca tggtccagat gcattgctac aggtatattt
      781 actctttctg cagcaacctt tgcatttgct agataataat caagacctgc atattttaca
      841 gtagatggag tagtttgtaa tattactgga gaatttaact cctcagcagc ttctataaca
      901 gcaattacca tttccatatt ttctacatta aatgctccta ctgcataccc ttcatcttgt
      961 gcctttttaa gcattgatgt tgttgttact aatgccacat tgataccctc ccaaattatt
     1021 tagttataat attttaatct tatcttttaa tattcatata ttgttacacc ttttactact
     1081 cgatttactg ttcctgtagc tgatggagta tctggcttat ttccaacctt taccgataca
     1141 aacaatgcta tagtttgtgc aaacaatatg tatggcattg caagatacac atctggtaat
     1201 ttattatact tttctttaaa ctcaaacttt gttcctgaga agttattttc attttctact
     1261 gacactgcac aagtgaattt agcaatttta tcccttttta tctcctctaa aacatctaaa
     1321 tcatattttc ttgtatataa acagttagat acaaattcaa atacaagtgt attttcatca
     1381 ataaatgatt ttggtccgtg tctaaacccc ataggagaat catacactgt agatattttt
     1441 ccggctgtaa gttctaaaag ttttagttgt gcttcttgtg tcaaaccacc taatcctcct
     1501 gaacctaaat atacaactct atcaaaatct ttatttatta actcatgtat ttcatctttt
     1561 ctatctataa catttcttcc catttcaatt attgcattta tatatgactt ttctacatca
     1621 tcttctaaac tatcgaatat taacatagca gataacatca tacacgaaaa acttcctgtc
     1681 atggcaaagc cttcatcatt tgattttgat ggcattaata acaagtaatt attttcgtcg
     1741 tttttagtca tcttagccag ttctccttct ggattacaag ttatagctaa atgataaaaa
     1801 ttatctacaa tcttattccc taaatttaaa gcagccaaac tttcaggact atttccactt
     1861 cttgcaaatg aaattaataa tgttggtata tcttttttta agtaatcata tgggtttgaa
     1921 actatatccg tagtaggtat tgcttcaaat atgtattttc ttatgtcatt tttattttta
     1981 aggtaaggaa gtattgaatt tcctatataa gctgaagttc cagcacctgt aaatataact
     2041 ctaaactgcc catgattatt acttattgta tctatgaatc tacttaattt ttctttgtta
     2101 gatttatata tttcataagt ttctaaccat aattcaggct gttgttttat ttcattagtt
     2161 gttattattg cacctaaatc ttttagtttt tgttcttcta atttaaacat tttcattctc
     2221 ctttcatact tgtaacgttt acatttggta atgaggttat aaccacttta aactataaaa
     2281 aaatatactc ttttataatt ttttgcttag attttttagt actacttaaa ctcactataa
     2341 aatatacact atagatttta attaatttta tagtttatct ttaaaaattt aataaaatca
     2401 tactctatat actttagtat ttttatttta cgataaaaac ttgttcagac caaagtggta
     2461 attaccacta atccaaatat aaattaattt gaattttttg tcaatacata aaatatttat
     2521 atcaatcaat tttatggata tcctacaaaa ttactttatc aatatttctt atttcgctaa
     2581 tttacttata taagctttaa atttattttt taatacaaag tttacaaatt tatgtgtctg
     2641 aattataatc aattctgtaa atataaaaac cttaggagaa agtattatga aaaatataaa
     2701 aatgggaatg ataggtcttg gctccatagc tcaaaaggca tatttaccta tattaactaa
     2761 gtctgagaga tttgagtttg taggagcttt tactccaaat aaagttaaaa gggaaaaagt
     2821 ctgtagtgat tatagaatca tgccattcga ttctattgaa tccctagcta aaaaatgtga
     2881 ttgtattttt ctacatagct ctacagaaac acactacgaa attataaaaa tattattaaa
     2941 tttaggtgtt catgtatatg tagacaaacc tcttgcttca acagtaagtc aaggagaaga
     3001 acttatcgaa ttaagtacga aaaaaaactt aaatttaatg gttggattca accgtagatt
     3061 ctgtcctatg tacaaagaaa ttaaaaataa tgctacagaa attgtatcta tcaatatatg
     3121 taaacatgga ttaaattcct taagaaatgt taaattcgac agtactctta tagatgacta
     3181 tatacatgtt attgacactg ctttatggtt agcaaatgaa gatgttgaaa ttagtggaga
     3241 ggatttattt ttaacagaca ataagaatct tatatttgta agtcataaac ttaaaggtaa
     3301 aaatttctct ataaacacct ctatgcatag agattctgga acaaagttag aacaagttga
     3361 aattctttca aagggtaata ttcaaagagt taagaatctt aatgtactgg aaatagaaga
     3421 aggtggaaac ctaactttaa agcaaagtgg tgcatgggta aacatattaa aacaaaaggg
     3481 ttttgaggat atatcaaatc attttattga ttgtatagaa aataatataa aaccagctat
     3541 taatggagaa gagtgtatca aggctcaaag attactagag aaaattataa attcagtaaa
     3601 atagatattt attataaaaa taggttaaaa atgaattttt ctaacctatt tttatataag
     3661 actatatctt ttatattaaa attaaaatct attacaacaa cattaaattt attatattcc
     3721 cattaaatgt atttaactta acttttttac ttcaactaaa tcttgatatt tatcaatttc
     3781 taccaaatct acaaatccag tttccatatt taaagcatta cttataccac aagcatttgc
     3841 aaatttaagt aaattttcta ttttataacc ccttaaaata cctacactaa atccagccac
     3901 tgtgctatct ccactaccta cagtatttat acactttaca actggtactt ttacatcata
     3961 tacattatct tcgtttaaat acaccatacc atcttttcct aaagatatac atacattctc
     4021 tgcacccatt tcaataagtt tttttcctga caagataatt tcttctcttg attctatatt
     4081 cattcctaat aaatgtctta tttcatctat atttggcttt attaaatatg gttttgattt
     4141 taaagctatt tttaatgctt ctcctgatgt atctaatata aactttatat tatttgctct
     4201 acatatattt cctatctttt cataatagtc tatcggcata ttctcacaat aactaccaga
     4261 agctaccagt attttagtat tttttagtac ttcctctata tcattttcaa atctctctaa
     4321 atcgcttttt tctactattg gtcctttttc taaaaattct gtactaactt tattgtcctc
     4381 aattatgttt aagcaatttc tagtttcctg ctctattttt gtaaatttat tttgtatatc
     4441 tagttttcta agttcatttt ttataaattc tccattaaat ccgcctagaa atcccatagc
     4501 taaaggattt tcttttaaaa ttttacatac cttagctacg tttaatcctt tacctccagc
     4561 agttgcattc ttacttacta ccctttgcac ctcacctatg tttatattat tcactctata
     4621 cattctatct atagatgggt tgaatgttat tactgtaatc atactagatt ctcctcatta
     4681 ttaattttta cttttctaaa caataaatta atctgtaaaa taaaaaatac ctcatataat
     4741 atatccaaag tggttattac caatttgaat ataaattatt ttacttgttt tgtcaatgta
     4801 ccataatttt ataataaatt aatagcctta cttattgaat tttgattaaa ctttattcat
     4861 tgacataaat aattctatat tctataatca aattgtggta ataaccactt agaaaggact
     4921 atatttttat gataaaaaga aatgataaaa gaccaatata tgaccagctt gttgagatat
     4981 taagaactaa gattgaaaat gagatggaac caaacgatag aatgctttcc gaaagaaaaa
     5041 tttgtgatga atatggagta agtagaacca cagttagact agctatggct gagttagagc
     5101 atatgggata tatatacaaa agacatggaa aaggaacttt tgttgctgca ctaagtcaaa
     5161 attctcaaaa cttaatggaa agttatagct ttacagatta tatgagaaca caaggaaaaa
     5221 agccaggtac aaaggtatta tcatttgagg tagtagaaag tacaaagtat ttctctgaaa
     5281 aattaggtat aactcctggt gaaaaaatga taaaaataat gcgtttaaga cttgctgatg
     5341 accttcctat gatgcttgaa agaacatatc ttcctatgaa agaatttata ggattgacaa
     5401 aagaaaaaat cgaacaaaaa cctttatatg aaatatttag agaagattat ggcgagatta
     5461 ttaaagttgc tgatgaagaa ttttcagctg gaataatctc tgataaggaa gctaagttat
     5521 tggaagtacc aggagattca ccttgtctga aattacttcg taccacatat aatgatgaca
     5581 atatagttat tgaatttact ttgagtgtcg ctagaagcga taaatttgta tatagagtaa
     5641 gacatgtaag atagtaagtt aaataacttt caagaaaaca gaatgatatt ataagcgtca
     5701 atgccacaat gatagtataa ttataaggag gttattttga aaagttttat ttatgcagat
     5761 aaatttttta tgaaacatgg aatcaaagaa aaaggttacc ttagtataat taatggcaaa
     5821 ttcggtgaat ttcaacaaga aagacctaat gttactgata atgttataga tttttcaggt
     5881 aaatatattg ctcctggctt agttgatact cacattcacg gtcttttagg tgcagatgtc
     5941 atggacaata catttgaagc aataaacaca atttcaaaag gcttattaaa atatggagta
     6001 acatctttcc tacctacaac tttaactgat tctgtagata cactaagtga ttctgtagaa
     6061 aatataagca atagcttcaa tcgagttgag ggtgcaaaaa tacaagggat ttatttagaa
     6121 ggtccatttt ttacagaaaa atataagggc gctcaaaatg aaaaatattt taaaaaccct
     6181 gacatcaata tattaaaatt atggcaaaaa aaatcgaaaa atttaataag aaaaatagca
     6241 attgctccag aacgtaaagg ttcaatagaa ttaacaagat atgcaactaa aaataatata
     6301 tcagtttctc ttggtcatag ttcagctaca tttgagcaag ttaaacaagt cgttaattca
     6361 ggtgctaaag tgtttgtaca tacatataat gggatgagtc ctttaaatca tagagaacct
     6421 ggaatggttg gggctgctat ggtactaaaa gatacttatg ctgaattaat ttgtgatggt
     6481 catcatgtaa gcccaatagc tgttaaaata atgatggatg ctaagtctag taataacata
     6541 gctttaatta cagattgtat gagagctggt gctatggagg atggaaaata tactcttggt
     6601 aagtttaatg taaatgtaaa aaatagtgtt gcaagattaa atagtgggag tttagctgga
     6661 agtgtattaa ctatggacaa ggctgtgaga aatatagttg attggaacat agccacttta
     6721 gaagatgcaa taaaaatggc tacatatatc cctgctttaa gttgtaatat tgataatgtt
     6781 tgtggctcca tacatcaagg tatggaggca gattttatag taacagataa ggactttaat
     6841 atatacgaaa cttatataag tggtatatgt atgtacaaag ttaataaata gattcaatat
     6901 aaagttatag aggcttccta aaaatagaga taagcctctt tttcattgtt aaaatgatga
     6961 aaatacattt gatttcatac aaaaagagtg cctcataaac taaaagtagg aaccttcc
//
LOCUS       DAENCV010000167         6996 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAENCV010000167 DAENCV010000000
VERSION     DAENCV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.170"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            62..442
                     /locus_tag="KQD82_003452"
     CDS             62..442
                     /locus_tag="KQD82_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG4381895.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            567..767
                     /locus_tag="KQD82_003453"
     CDS             567..767
                     /locus_tag="KQD82_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381896.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            963..1691
                     /locus_tag="KQD82_003454"
     CDS             963..1691
                     /locus_tag="KQD82_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4381897.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1713..2630
                     /locus_tag="KQD82_003455"
     CDS             1713..2630
                     /locus_tag="KQD82_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG4381898.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2772..3956
                     /locus_tag="KQD82_003456"
     CDS             2772..3956
                     /locus_tag="KQD82_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG4381899.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4063..4251
                     /locus_tag="KQD82_003457"
     CDS             4063..4251
                     /locus_tag="KQD82_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG4381900.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4400..5050
                     /locus_tag="KQD82_003458"
     CDS             4400..5050
                     /locus_tag="KQD82_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG4381901.1"
                     /translation="MKKNYEIILFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLDNLE
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            5153..6091
                     /locus_tag="KQD82_003459"
     CDS             5153..6091
                     /locus_tag="KQD82_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG4381902.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6117..6968
                     /locus_tag="KQD82_003460"
     CDS             6117..6968
                     /locus_tag="KQD82_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG4381903.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         2883 a          660 c         1266 g         2187 t
ORIGIN      
        1 ggtatattat taaatataaa aaatattagg tatttattta ataataaagg tgggtattgt
       61 tatgataaat aaaataggaa aaataactct ctatgtaaat aatcaagagg atgcaaagtt
      121 attttggaca gaaaaattga actttgtagt aacttatgaa aaggaaatgg gtccaaatat
      181 gaaatgggtg gaagtagcac caagtaaaaa tgaatttaca acatttgtgt tgtatgataa
      241 aaatctaatg atgaagcaaa acaaagagat aaatgtatct catccatcaa taattttaag
      301 tacaagagat attaatggaa cttatgaaca aatggcaaat aacgaggttg aggttgggga
      361 attaatggag atgccatatg gaaggatgtt ttcttttaaa gaccaagatg gaaatgatta
      421 tttggttaga gaggataaat aattttacaa agtattaaat ttactcaata tctgatataa
      481 ttagagacaa taattattat aaactagtct aggattagaa ttatataatt aaaaattaag
      541 tgtaaatttt gagtagaggt gataaaatgt ttaagaaaat ggcagtacta aaagatatag
      601 caactaaaat aggtcgtaaa aaagcgtatg aactattaga aatggttgaa ggtaatgatg
      661 cctttgtagc tgaggtaaag ataaaaaaga atggaataga atctaaaaaa gaagaaatta
      721 tgttaaaaga taatcaaaaa ataatattag agtatataga aggttaaatt ttaaatacat
      781 ataagttgat atactaaatt tctgagatga gtttacataa aactcatctt ttttactttt
      841 caataacttt gttttattat ttaatataca ttttatctcc ttagagttgc tgatatataa
      901 aaattactga tataataaaa gaatggatat taatgtaata ttttatagaa aagaggggta
      961 ttatgcttga aataaaaaat ctttccttta atgttgagtc taacaatgag gaactaggaa
     1021 taataaatga tgttagcctt tcatttgaga ggggaaaact tattgttatc acaggtccaa
     1081 atggtggagg aaaatctaca attgctaagc ttattatggg aattgaaaaa gctacgagtg
     1141 gtcagataat tttagatgga gaagatataa caaatcttag tattacagaa agagcaaaaa
     1201 aaggaatagg atacgcattt caacaaccac caagaataaa aggtatgaca gttgaaaatt
     1261 tacttacact tgctcatgga aaaccattgt ctactgatgt atgttgccaa taccttacag
     1321 atgttggatt gtgttcaaaa gactatttaa atcgtgaagt tgataactct ctttctggag
     1381 gggaaatgaa aagaattgag atagcaactc tttttgctag agatttaaag gtttctatat
     1441 ttgatgaacc tgaagcaggg atagaccttt ggagctttgg aaaactcaat gaaagtttta
     1501 agaaaataca tgaagagtct aatcagacta taatcataat ttctcaccaa gaaagaatat
     1561 tagagttagc tgatgaaata attgttctac aagatggttc agtaaaaagc catggaacaa
     1621 aagaggctat attacctgaa atcatgtgtc aagttaattc aagttgtgaa ttaatgaaag
     1681 acatgaatta attagaaagg aaggcaaatt aaatgaacaa aatagattta aatcttttag
     1741 aaaaagttgc tgaacttcgt ggaactccat taggagcata caatatacgt aaaaatgggc
     1801 aaggagtaga gagacattca actgaaaatg taactataat tcctaaaact gataaacctg
     1861 gaatagatat tatagtaaag ccaaatacaa aaggagaaaa tgtacatata ccagtaatag
     1921 taacagaggc tggtgtacag gatatggtat ataatgattt tgaaattgga gaaaatgcag
     1981 atgttgttat aattgctggg tgtggtatac ataactgtgg ttgtgatgat agtgaacata
     2041 atggtataca tagatttttc ttacgtaaag gagctcgcct aaaatatgta gaaaaacatt
     2101 atggtgaagg tgatggagaa ggaaagagaa ttttaaatcc agttactatt gtcaatatgg
     2161 atgaggattc atattgtgaa atggaaacaa ttcaaattaa gggtgtagat tcaactaaaa
     2221 gagaaatgga tgcaaaccta ggtaaaggag ctaaaatagt tgtatttgag aaattattaa
     2281 ctcatggtaa tcaagttgca gaatctaata tgaaggttat cttagagggt gaaaactctt
     2341 ctgctcaagt agtttctcgt acagttgggc aagataattc taagcaaaag tttaatcctt
     2401 gtgttattgg ggattccctt tgttctgcac atgttcaatg tgattcaata attatgggaa
     2461 attctcaaat tagtgctata cctgaaattg ctgcaaatca tccagatgct cttttagtac
     2521 atgaagcagc tattggaaag attgcaggag accaaatact aaagatgatg acattaggtc
     2581 ttactgaaga agaagccgag caacaaatac ttaattgctt cttaaagtaa attaaagatt
     2641 ttatttaatt taatataatt aatatagtat aatatagtga ataagtctaa aatattttta
     2701 tttttaggct tattttcttt atgtaagaaa agctattaga gaaattatat tgtaattagg
     2761 atgtggaata aatgaaaaat ttacttataa gaaatgggac aataatatct ccaacagatg
     2821 gtatcaaatt tgaaaaaaga gatatcttgg ttttacatgg gaaaatatat gctattggat
     2881 tgaaccttaa agagaaaata agaaaagaaa aattacttac aaataaagag ttcgagataa
     2941 taaatgccga taatatgtat atttccccag gatttataga tgttcacacc cattgttata
     3001 agagaaaatt acctacaggt atggattctg attcaattgg aattgaaaaa ggttctacag
     3061 ttatatttga tgcaggtaca gctggtccct caaactacta tgattttaaa aaaaagtata
     3121 tggatgagtg taaaacagaa gtatattctt ttttaaatgt tacaaatgag ggactaaata
     3181 tattgaatga atcaacttct ttagatgtta taaattttga caaggttgag tcaattgtag
     3241 aaaaaaatat agaaaagata aagggtataa aagtaaaagc gagtaaattt caatctaatg
     3301 aaagtggtat agatataatt aagaaatcaa agcaagttgc taaaaaatta aatttaccct
     3361 tagtggtata tataggagag tatccatcat atatagatga agtaatgaat attttgggaa
     3421 aaggagatgt ggttacaccg gtatatggaa atagtacaaa tggaatcctt aaagaatctg
     3481 gcacattaag aaaatcagta ataaatgcaa agaatagagg agtattgttt gatgtatgtc
     3541 atggagtgga ccaatttgat tttaaagttt ttaaaaaagc aataaagcaa ggtttagagc
     3601 ctgacctaat aagtactgat atgcatataa aaaacatgaa agacgtaaac tattctcttt
     3661 taaatgttat aaataaaatt atggaacttg ggatatccct agaaaagtgt atagaaaaag
     3721 taactgaata cccaacaaag attttcgatt tacatggaaa taaaggcaga ataaagattg
     3781 gtggagaagg tgattttaca atatttacaa tggaggagtg taatgatatt ataaaagact
     3841 acaataataa tgaacttgtt ttaaataaaa aattaagatt gcaatatact gtaaaatcat
     3901 ggatgaaaag cagtgaagtt tatagacatg gatacgaaaa tactataata aactaaacaa
     3961 tagtgataaa ataataaata tatgtttaaa ttggtatata ttggataaat atctaaggaa
     4021 acaacaatta aaaaataata attttagggg aggtatttta gtatgaaagc atttgttgat
     4081 aaggatgtat gtgtagcttg tggagcatgt ataggaacat gtccagacgt ttttagtatg
     4141 agcgatgaag ggcattcagt agcaattgaa ggagaaatac cagcagattt acaagattta
     4201 tgtaaagaag ccgaagaagg atgtccagtt tcagctataa cagttaaata gagctgttta
     4261 aatttatttt taaaataaat atataagatt taattggtac ttaagctata gtattagaaa
     4321 cttctattac tatagctttt taaaatagat aaacttaata tataagatat tactttttat
     4381 aaatatggag gactgaaata tgaagaaaaa ttatgaaata atattgtttg atttagatgg
     4441 aacacttaca gaccctaaag aaggaataac aaagtctatc cagtattctt taaatagctt
     4501 tggcatagaa gaagatttgg acaacttaga acaatttata ggaccgccat tacatgatac
     4561 ttttaaggaa tattataaat ttgaggataa aaaagctaaa gaagctgttg aaaagtatag
     4621 agaatatttt gctgataaag ggatatttga aaataaaatt tatgagaata tgaaagagat
     4681 attagaaatg ctctataaaa atggtaaaat attattagta gcaacttcta agccaacagt
     4741 ttttgcagaa actatactta gatattttga tatagataga tattttaaat atatagcagg
     4801 tagcaatctt gatggtacaa gagtgaataa aaatgaagtt atacaatatg tactagacct
     4861 atgtaatgta aaagataaag ataaagttat tatggttgga gatagaaagt atgacataat
     4921 tggagctaaa aagataggta tagattctat tggagtattg tatgggtatg gttcttttga
     4981 agagattagt gaaagtgaac ctacttatat tgtcgaaaat gtagagtcta taaaagatat
     5041 tttgttataa atatctttta tagacaaaaa tattacaaaa tggtgtcgca tatttaaaaa
     5101 tatgatatta tagttaagga agtcatttaa aaaagaggag gatgcactta aaatgaaatc
     5161 tattttaatt atgggtggaa gcgacttcat aggttctgca cttgctaagc gcttgattaa
     5221 atgtgggtat caaattgata tacttacaaa tggtaaaaag gaaatagatt ataatggttt
     5281 taaagaacat ctaatttgtg atagaaaagt tcgaaaagat atggaaaaca ttatcacagg
     5341 aagaaaatat gattacatat atgatatgac agcatataca aaagaagatg taagtaatct
     5401 gattgatttt ataagcatgg ataacttaaa aaagtatata gtattgtcag caggagcagt
     5461 atataaagat agtggaagaa atataaaaga agaaaatgag aaaggtgaaa atgaaaactg
     5521 gggaaaatat gggttaaata aaaaagaggc tgaagatttt attataaata gccctatacc
     5581 atatataatt attagaccaa cttacatata tggtgaaaat aataatttat atagagagta
     5641 ttatttcttt gaaaaaatag aaaaaaatga aaagatacca gtaccaaaag gaaaacaagt
     5701 tagtaatcaa tttatatata ttggggattt ggtaaaggta ttggaaagca taatgaaaaa
     5761 tcctcatgta agagaggcct ataatgtgac aaatccacag ttaatttctt gggatgacct
     5821 tatatataca tgtggagaaa ttattggcaa agaacctata ataaaatatg tagatatgga
     5881 aaaagtagag tttagagaaa gaacgtattt cccatttaga aatatagact ttaatcttga
     5941 tataaataag cttattgaac atgggcttta tataccaaac gtgttactaa aagaaggtct
     6001 tacagcaaca tataaatggt tttcagcaaa taagccaaaa atgcatgaca gaaaaatgaa
     6061 caaagttgag tctgtaatgc aaatagttta aaaaaataaa taagtaggta gatattttga
     6121 actcaaaaat taagtatttt ttaaaatttt ttatcttaat atttattttg atttttatat
     6181 actcagttgc gatagagccg agtctattaa ttgtgaaaaa atataatata agtgaaaatg
     6241 gagcattgat aaaaaatgat acaattgaga gtaaatctga aagtgtaaaa gtagttcaaa
     6301 tttcagatac tcaaataggt agtttttatt caactaagaa tctcaaaaaa gtagctaata
     6361 agattaacac tttgaatcca gatataatag tatttactgg tgatttaatt gattactcca
     6421 ataaaaatcc aagtgtagat gaaattacaa ctattttatc aagtatgaat gctaggttgg
     6481 gaaaattttc agtatttggc aatcatgatt atatgtataa acttcctagg tattataggc
     6541 agattatgaa aaattccaac ttcaatcttt tagtaaatga aaataaaaaa ataaaattaa
     6601 aagatgataa atatattaat atattaggtg ttgatgaaat attaaatgga aatcctaata
     6661 taaaatatct ggagagtcaa atagataata aaagttttaa tttattgtta gcacatgaac
     6721 cagatttagt agatatgctt agtaaagata caatgaattt agttctatct gggcatagtc
     6781 atggtggtca aataagatta cctattaagg gagcattagt tactcctcca tatggaagga
     6841 aatatactaa agggttttat gatataaatg gaaatcattt atatgtgagt tcgggtcttg
     6901 gaagtacaaa gttaccattt agatttttta atataccaga gataatagaa tttaatatta
     6961 taatctaaca taaaatattt agaaaatata attttg
//
LOCUS       DAENCV010000168         6954 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.171, whole genome shotgun sequence.
ACCESSION   DAENCV010000168 DAENCV010000000
VERSION     DAENCV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.171"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            240..1067
                     /locus_tag="KQD82_003461"
     CDS             240..1067
                     /locus_tag="KQD82_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG4381904.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2089..2553
                     /locus_tag="KQD82_003462"
     CDS             2089..2553
                     /locus_tag="KQD82_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4381905.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRALKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2820..3464)
                     /locus_tag="KQD82_003463"
     CDS             complement(2820..3464)
                     /locus_tag="KQD82_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381906.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGGVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3394..4683)
                     /locus_tag="KQD82_003464"
     CDS             complement(3394..4683)
                     /locus_tag="KQD82_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381907.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIIINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4680..5552)
                     /locus_tag="KQD82_003465"
     CDS             complement(4680..5552)
                     /locus_tag="KQD82_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381908.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQSLIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEEKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            complement(6081..6572)
                     /locus_tag="KQD82_003466"
     CDS             complement(6081..6572)
                     /locus_tag="KQD82_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG4381909.1"
                     /translation="MGMIGCYTKISEENVFKLQQSEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT         2542 a          950 c          823 g         2639 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacgaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acaccttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatattttt aaagattctt tttgtaacta ctagaatact aatttagtag
     1681 ttattgtatc tctaaagttt aagcatggat agatatacat ttgataattt gcctataatt
     1741 tacctactac ttggagtatt ggagcaaata caatatatta ataataagtt cctaaagcca
     1801 ataaattaac taatacacat aaagatattg atgttgatta tacaactata agaaacggat
     1861 ttaacagaat ttaatcaagc taagaaatta tatatatcgt gatgtttttg aattagaagg
     1921 taattcaaaa ttagtttata gtcttctgtg aatccaataa aaagtacagg gtacaagaat
     1981 tgttagtaaa atttttaaag attttatgga taaatataaa tagttttact tattttctaa
     2041 aactttaaaa taccattaat taccttaaat attttaatag gaggacatat gaatttaaat
     2101 aaagttaaat ttactgaaga acatgctatt gaagttacta attggaaata tgaaggtaaa
     2161 tattcaatat acaatctacc tccatgggag gaaattaaaa agaagaattt ttcactagca
     2221 aaagaagata aaagaaaaaa ttttataagt tttataaatg acaataaaga attgataggt
     2281 tttataagct tattagatga aggaagctct gtattctttg gtattggtat gaaaccgaat
     2341 tattgcggaa tgggaattgg taaagaaata attggcctag gattacaaga atgtagaaat
     2401 aaatatagta ctaaaccaat tgtattaaat gtaagaacct ggaacatgag agctttaaaa
     2461 tgttatgagt ctcaaggatt taaaatagtt gagactaagg tacagaaaac gcatttagga
     2521 gatggagaat tttttgttat gaggtatcaa tagtttataa caatagtctt attgttaata
     2581 caacaattaa aataaaaaaa tacatatgtg taggtcatag caataagtgt ctttgaaata
     2641 taaatttgag agcttttgat aaaatttggt atacatatat attttatact acggatatat
     2701 aaatctatct acatacataa agagattgta agtaggtttg tgtatataaa aatagattta
     2761 tatttcataa atgtaccttt tatttgtatt acctttattt tatctaagct ctcattttat
     2821 tatctaatct tcctttttac tggttttatt aagagagatg caattatcaa cacaacactt
     2881 ccaagtatca gccaatattt ctcatcaaaa ctaccttgac tcatagacat tataaaaaat
     2941 tttccaacat acttgccttt agtcatcata tttaaaatta aatatcctat agatatcata
     3001 taacctatga ttgtattatt aaataaattt gagaaaaaca ttcctaaact tcctataaac
     3061 atagctgatg cgaaacttcc aataatatac tcaactactg gaaaattaca tccaagatat
     3121 attaaaacat ttacaaaacc tatgattaat accactatac ctataattga catcagtatc
     3181 ctaattaatt gtacaataac ttgagataat gcttttgaag atactatctc atcaatttct
     3241 tttgattgct ctagcgaaaa tacaggtacc aacattactc cccctattat tgcaacataa
     3301 ttttctaaac atatagctgt tgaaattctg tctaaattag ctactccttg tattagagga
     3361 atcatacata gcaatataat tgaacatatt aaattagtta aagtgcttaa tttgaagttt
     3421 tcttttatta gaactaaaca gttttgaaaa ctttgcccaa acatcaagat tcccccttct
     3481 tttttgctca tataagaata tagctaacaa aacaagcacc aatgctccca agcttatcac
     3541 aattctatta attataattt gattgaagtt ttgtataata atatctccat ttaatacact
     3601 attatgtctt atcattagcc ccattgttga ataatcacct ttgagtcctg tagaatttat
     3661 actgtaaaac caccaaatta attgcacaat aattgctact ggtgtatctg ttaatatagt
     3721 aatactcatt cctacagcgg taactatcat aagagttggt agtatccacc ataatgtata
     3781 ttttataaat gctaagttat caatagatat acctttatct ttagcaaaga aaatcaaagg
     3841 aagtaaatct ttcataccta aaattaatac aggcaatatc atcataaaga caagtgcaaa
     3901 atatcttgat aaaactattt tgcatgaaga tacttgtttt gaataaataa tctctctcat
     3961 tcttgaacgc ttatctttaa gtgacataaa aactgccaca aatactggaa atatacttaa
     4021 aactatacct atataatcgc aaaaaattct tgcataccca ttactaactt tatctttttg
     4081 tattgtatag ttgtaattct tcatagcttc atcataggta actggaacta ctccaaatct
     4141 ttgtaacttg tcaaaagcat atgcagaccc tcctcctaaa atgtcgtcta ctctttgcat
     4201 tatttcttca aatctttcaa gagatacttt agaataatct atttttatac tttcatcagt
     4261 tttccaaagt gaatttattt cgctaatact tttaccagtt atttcttcta tatatttttc
     4321 tatggtcttc gttttatttt ggtctaattt tatatactta actacccctg ttggatacgt
     4381 tgtatatgtg tttttttgat attctctaat taggtttata gttgcatttg gaatgacctt
     4441 ttctgttttc atagattctt tttgaccata tggatattga ttgtgtacag aataatattc
     4501 ctcaatattc tctacttttt gtggagcaac taatggattt ccatcattag gaatattttt
     4561 acttacatca tttgaaactg aacttccaag ttgagaaata tagaaaaaaa ctaaaactac
     4621 aataaaaata taatatatca tactctttaa tatattttta cattctttta taaatagatt
     4681 catatattat ctcctcccat tataagcata tagccatctt caatagttgg attacaattt
     4741 actgctgtct caaaaggttt atcatctgat ataatcctaa gtatagcctt tccaccatga
     4801 gtcaacattc ctataactat aaatctactt tttatatttt caatatcttt tttatctact
     4861 tcaatactat atactttctc ttcagctagt tttgctaatg cttctgttga gccattgtat
     4921 atgacttttc cactattcaa tatagctacg ttttcacatg tagcttctat atctccaaca
     4981 atatgtgtag ataaaataac gattctatca tttgcaattt cgcatagtaa atttctaaat
     5041 cttatacgtt cttctgggtc aagacctgct gtcggctcat ctactattaa aactctggga
     5101 ttatgaatta atgattgtgc tatcccaagc cttctattca tacctccaga taaagatttt
     5161 actttcacat tttgataatt agtaaggttt actttgctta ataactttgc tataattttt
     5221 cttctttttc ttagtggtat atccgataaa actgctaaat aatccattgc tttatatact
     5281 gtcatgttag gatacattga aaagtcttgt ggcaaataac caaccatatt tctaatttcc
     5341 ttagtattct caataggaac tccacacata tgtattgaac cttctgtttt tgataataaa
     5401 gtggctaaaa ttctcataaa tgtggttttt cctgctccat ttcttcctaa aagaccaaac
     5461 ataccactgt cgatttcaag atttatatta tcaagtgctt gttttttccc aaatcttttg
     5521 ctaagattgg atactgctat tttcatttcc atatataaat acacccccag aaacaatttt
     5581 atagaatttt atttttataa atttttactg tattttcata ataaattcta aaacttaaga
     5641 tattatttat aaatttctaa attttttcta aagattttaa tttttctgtt tttaattaaa
     5701 ttaaagtatc tttgtttctg aaatttttta agtttatgaa gttataattt aatatatatt
     5761 tattttattt gtgagtctat ttttatatat aatgaaaaat gtgataagta tccaaaagaa
     5821 ttactggata taataagata gggtatttag ccttttaaga taaataaaaa tatctgcaac
     5881 tatatattgc aaaaatttaa aactaattta tatatgctaa tataaatatt tataaagaca
     5941 taagacaaat ttacctacag atttaaattt aacttatata ttttaaaagg ctaatataat
     6001 atgtattttc ttctatacaa tcaaaaggct tcatatctaa gatatgtgat gacactaata
     6061 ttcacaattt ttagtaatta tcacattata aaaaatatta cagctagtcc atcattagat
     6121 gcttcttgta taaacttttt aagctcaatt agatttgccc atacatattc aaaaaaatca
     6181 tcttcactct catcattcat aacaggataa atattatttt ctaacatctc attaatatta
     6241 aatctttttc taaatgcttg ttcttccaaa ctgttcattg cttttgatag tttttttaca
     6301 tcatttgctg taatcaacat gacaggaaat tctccatcaa tttccataag tatgtttcca
     6361 cttagtacta atttgctaaa tatattatca tcatctccac caaaaggaca acctgtcaaa
     6421 gtaaagtgaa tggcatgcca tgctttatca atattgatag tactattttc gttggcatct
     6481 tcaaaaacaa aatcttgtaa attttcttct gattgctgta atttaaaaac attctcttca
     6541 cttattttag tataacatcc tatcatcccc ataattacat tcctcctaag tagcgttttt
     6601 ttaattgtaa cttataatta aacttagtta caataaaata tattgagaaa agtattattt
     6661 catcttaaat ttaaatacct atattatcaa ctaaaattct gtgtttatag cgtaagtatt
     6721 attatttttt cctaaaaatt aaatgaatca gaagttctat aggcaatatt gactataaat
     6781 aaggactatt acttttaaaa aaaatttaag aaattatact gtagaatcat gctctactta
     6841 ttagcttcaa ataaaaaata aatcatgttc tcaaattaaa taataaataa caataaattt
     6901 aaattttaaa ctataagagt ttctgaagtg ttaataagca taccaataat tata
//
LOCUS       DAENCV010000169         6912 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAENCV010000169 DAENCV010000000
VERSION     DAENCV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.172"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            208..1089
                     /locus_tag="KQD82_003467"
     CDS             208..1089
                     /locus_tag="KQD82_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4381910.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1280..1447
                     /locus_tag="KQD82_003468"
                     /pseudo
     CDS             1280..1447
                     /locus_tag="KQD82_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1707..4274)
                     /locus_tag="KQD82_003469"
     CDS             complement(1707..4274)
                     /locus_tag="KQD82_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG4381911.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4277..4882)
                     /locus_tag="KQD82_003470"
     CDS             complement(4277..4882)
                     /locus_tag="KQD82_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381912.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5256..6716)
                     /locus_tag="KQD82_003471"
     CDS             complement(5256..6716)
                     /locus_tag="KQD82_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG4381913.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
BASE COUNT         2176 a         1312 c         1022 g         2402 t
ORIGIN      
        1 tttaattatt taatttttat tatttgtact tatttaaagt ttcatctaat tataaattgg
       61 acaattttta tgttgatttt attcatacaa attaaagtaa cttataaaat ctccacaata
      121 aaaaacattt tttattcact aaactgctcc atcattggtt tagcaataaa tatatttttt
      181 aggtctttaa tgtcaatagt gttaaatatg ccagcatatt tatttgatag taattctatt
      241 gcaaatattt acagagtatt tcctatatca tttagcttta tagttacagg actatatttc
      301 agttataaaa ataataaaaa taataagatt aactttgagg cagttttaga ggacaaaaaa
      361 aacttaattt tcttattgag ccttatgttt ggaatgtact tgtatctatg tttgagtttg
      421 tcaatatact atactgttgg aaatgtattg attttaaaat tatatggaat caagtctgcc
      481 ataatagttt tggtaggtaa ctatattgca gaaagatata gctttagaat tactcaaata
      541 aaaggttatg atattaaaaa tcaaatagaa tatgtagaaa gtataaagac agagtatgaa
      601 attaataaat tgaatgatat tgtatataca gatttactga caggcttcta caatagacct
      661 tttgtaaatc aaaaactatc agagtggaca tcaaatcact acaaatttac cttatgtttt
      721 actgatttaa atagcttaaa acctgtaaat gataaatttg gacaccaatt tggtgataaa
      781 tacatatcca ctactgctca aataataaaa aaacttataa atgtagaaaa ttccttacta
      841 tttagatatg gtggagatga atttttattc cttttacaga atacaagttg tgtagaagca
      901 gaaaaaataa tgattaatat aaattatgaa ttatcaaaat taagtaatac tgacgagtat
      961 ccttatgctc tttccataag ctatggatta gttgagtggg atggaatatc cgatattgaa
     1021 actttaatat caaaggcaga ttctttgatg tatgaaaata agcttaaaaa taaatctact
     1081 ttaaactagc tagttcagat attctacaaa atatatgtta taagagagta ctaagaatgt
     1141 caaaatatat agattttgca aatattgaat acagtttact aatgaatgca ctggatgtaa
     1201 gtgttagtaa acacctatta gatgaacact ttactgttgt ttgggctaat gaccattatt
     1261 atgaaatgtt cggctatacc tagctaaaaa gttaaaaatg accactgtag ccgagggaat
     1321 agagaccagt tttcaaaaag atttcttaca agaaatagac tgtgatatgt tacaaggata
     1381 cgtgttctct agacctttac caataaaaga ctttgaaaaa cttatgtttc aaaactcctc
     1441 caattaacaa tataaaattg cttattataa gtgatattat tgtatcaaca aataaaggaa
     1501 ttgtctcaaa attgattttt tctaatcaaa tttttatttt gagacattct catttttttg
     1561 cataagaaaa tacccaccaa gctacggttt ttcttaccgc tgttcggtgg gtattagtag
     1621 tattatgaaa tctgaaaact ttaagtatta ctacttccgc tagcgcaatc acaaaggtcg
     1681 aaagcttcaa agcgaaaata ggctttttac tcatcaaacg tcaccgtacc tgccaaccca
     1741 tccaccgtta tcatttgtcc gctcttaata gttcgtgtgg caatgtgtgt agccataacg
     1801 gctgggatgc catattcacg tgcgacaatg cttgaatggc taagaggacc accaatgtcc
     1861 gttacaatcg cacttgcaga tgaaaataat ggtgtccatg ctggagttgt ggtaactgca
     1921 accaaaacac ttccaggttg aaagctttca aaatctgctg gactattcag tacacaagct
     1981 ggagccgtca caacaccagt actggtacca atgcccgtca acacaatttt cccatctttc
     2041 tgcttctgtg gtgcttgtga tatagttttt ttgttttttt ctggcaactt ggatggtgac
     2101 acataaccca tgtatttttt aagttcagct tttcgaacag gtattatccc gttcatatca
     2161 gatagagact tgtttttatc caactgctct ataagtacct ccaattccga ttttgtgagc
     2221 caataaatat catctaaatg tgaaatagct cctcctcgta tgaatcgttc tgagatttcc
     2281 tgaaacatac aacgaatcag aggatgtccc attcccatca aataaatagc attttcacgc
     2341 ataggagagg tttcctgtgc ccaatggagc aacttaaaaa atagtttttt acgcaatcca
     2401 cctatgtgtt gtaaaatctc ttcttctgca tgttttctac gcttttcaaa cgtggtctgg
     2461 cgtaaaaaag gactttcccc ttttccttcc acaaatgttt ttacggattc aaaagttggt
     2521 gtaagtgttt cctgcggtgt ggaatgcgca aaatcaaact cataagcggt acgaccaaac
     2581 tctttaaagt acttattgat tcgatttttc cattctatcc atacttcctg tgaaactcca
     2641 acaggcacaa ctgaagacat gaaatcttct gctatcttta ttgcaggact gttttgcaga
     2701 taaaggttga tgctgttatt ttgctttacc cattctgaaa taccccacaa ctccttttcc
     2761 gattgcagaa caatcgtatc aaacccaagc aggaaaacgg aagtatccgt tataccagca
     2821 cgtcgaactg ctccttgaaa taattttgta aataatgttt cactgataga agcagcaggc
     2881 aatgtaagct gaatccttgt gaaataaata catgcggcat aaaatattgt ctgaataccc
     2941 tccataattt gatgggcgtt catagtatgc aggggctttt cttcccattg tttaatagca
     3001 tcctcatatc cttttcgtgc cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt
     3061 aaagagcgtg gggaaaggga tttgatagca atcaaaagtg gatttgattt agcagaaaga
     3121 taaacatatc cattaataac cgtatatgcc ccgttttttg gtaataattt tttcgcactt
     3181 ttaccaaaca tatctatcca taaaagctct gatgcacggt ttacgatttc aagaccaagc
     3241 gtggcgaaca aaggtgtaac aggatttggc aaatgttctg ccaaacttcc ctttgtgtat
     3301 acaacatcct gttctggcaa tacccattct ggtggcagaa cagtaattgg acgagcctga
     3361 acaatgtaca atttatcttt ttccagtgcc cactctacat ccataggcat ttgataatac
     3421 ttctcaattt tctttccaag ctgtatcagt tgcattacct gattacgagt aagagcatat
     3481 ttttttctca acctttcagg aaccatggtt tcttctgttc cttctgaagt acgaaccgtc
     3541 ataatctctt tatttgccac ttcatacgat ataattcgtt cggaatcctt atccactacg
     3601 attgtatcgg gtgtaactaa gctagaaacc accgcttcac caagccccca tgcagcgtta
     3661 ataatcattt cgctacgtct accattgatt gggtttaacg taaacataat accagatgaa
     3721 tcggaaaatg caagcttttg tactacaaca gcaagtgcga caatctcgtg ctttatatta
     3781 ttcttcacac gataagcaat agcacgagct gtccataagg aagcccagca tctctttaca
     3841 gcgtcaataa ctttatcttc accttgtatg ttaagataag tttcctgctg acctgcaaaa
     3901 gaagcgtcag gtaaatcttc agcagtcgca gacgaacgga cagccaccga tatgttccct
     3961 aatccggcat aagctgtttt aattgcgtcc gacacttcct gtggcatttc tccattatgg
     4021 aaaagctccc ctattttctt agatacatct tcaagttggc tggtattatt ggaatcaata
     4081 ccatctagca gtttattaat tcgagactga atatggttcg tctcaacaaa aatctggtat
     4141 gaagcagtcg taacatgaaa accgtctggc acagggatgc ctgctgtcaa tagttttgat
     4201 aaagacatcc cttttccacc aacaatttca atggttgctt gtttatgttc cagtggcaag
     4261 acataataaa tcataatcat tcacctccat cattcataat tccgtgcaga aatatattca
     4321 ttgtttcatc caacattttt tccgaactat ccaatggtct tgacgtgtaa ctgattgata
     4381 acatggagtt aattaacaaa cgtgtcacaa gagcggtatc cgttttgcga aagatgcctg
     4441 tggtcacacc attatcaatt accgatttta taatatcttg atatgcataa cgagcatctt
     4501 taagtctctt tcgatactct tcttctaaat aatcagattc tgtatttaac cacatcagta
     4561 cagttctata ctgctctgga aatctaagat gattaagcat gatttttcta aggcactgtt
     4621 gtggtgaagg ttcaccatca ataatcctat gaacttgttg gatagttttt tctatttctt
     4681 tctcaactgc atatacaaca atctcatctt tcgttttgaa aaaatcgtat agacttgact
     4741 ttcccatgtt ggctaaaact gcaatttctc tcattgatgt tttttggaat ccattttcag
     4801 ccatgagact caaagcaaca gcaactattt tttctcggcg ttctgcttgt tgttcgggtg
     4861 taagcacaat tccttttggc atagggcgat acctcctttt agcgaccact ggtcgtttct
     4921 tttttattta tcatacagcg accaacggtc acttgtcaag gttaattttt ttcattgtat
     4981 atggtactcg agataaaaat gttcatttta gaaattgctc tatttaagta gaaataggct
     5041 tcatcattag aaaacaagac tataaatttg agatatttat catgttcaaa tttcatcaat
     5101 atcaaagttt aaaatattaa taaaaaaaga attactaaga aataacatat aaagattcaa
     5161 aaaaggagtt gaacttaaaa tttaattaca aaaaatcaaa tttaagacaa ctcctttatt
     5221 ctttatctct actattcttt atctctattt atttattaca actccctaac atttaccact
     5281 actgccttag gtctagtcat agcctcaatt gaatacttaa caccttgagt tcccatacca
     5341 gatgccttta cacctaaaaa tgggaagtgg tctggacctc tttcagtctt attatttatt
     5401 tgaacagtac caacttcaag tttatcagct atataaaatg ccttatcaat atcacttgta
     5461 aatactgatg attgaagccc atactctgat tgattagcaa tttcaattgc ttgatttata
     5521 tctttaactc ttataattgg aagtactgga ccaaatggtt cttcccaagc aattttcata
     5581 tcaacattta cattatctaa caatgttgga tagaataaat tattttttct aacattgcct
     5641 gttattaact ttgctccttt atgtaatgca tcatcaacca acccttgaac aaaatcagtt
     5701 gctttattat ctataagagg agtgataact acttcatcca ttggatttcc aattgtcaac
     5761 ttctctacca atggctttat ttttccgact aatttatccg ccacactctc ttgtactaaa
     5821 attctcttta ctgccgtaca tctttgtcct gaatatgaat acgctccaga tactatattc
     5881 ttagctgcaa aatctaagtc tgcatcttct aatactatag ccgcatcttt tccaccaagc
     5941 tccaataata atggtaccat tccagatatt ttagatatat gctgaccaac ttcagtacta
     6001 ccagtaaagt taataaaatt aattccttta tgcgttacaa cataatctcc tatctcacta
     6061 cccctacctg taatagtatt aagtactcca tctggtatcc cagcttttct cataatttct
     6121 accacatgaa gtgcgctaat agccccttgt gtagcaggtt ttaatacgac tgcatttcca
     6181 ccaataagtg ctggagcaat ctttgacatt gataaattta caggataatt aaaaggagat
     6241 atagctaaaa cagtacctaa tggaaccctt gaaacataag acatcttatt tcttgttcca
     6301 ccagggaaat tatctccact aatggcttca ccctccatat tttttcccac atcagctgtg
     6361 tatcttaaaa aatctgctgt tctctcaact tctgcccttg cagattttat gtctttggct
     6421 atttcaagta ccagtatatt agctatctca tctatatttt ctaacaataa atcagcagcc
     6481 ttatgaaata tattggctct cttgtataca ggcatttcag cccatacttt tatactctct
     6541 tttgtgtttt ttataacctc atctacctca tgttttgtca tagattgtac tttcccaaca
     6601 agagaattat ctattggtga gtaaatttct attggctttc tcgaattaga ttctacccat
     6661 tttccattta ttaaattttt atatacattt tcttttgttt taagttcatt aaacatattt
     6721 ttattcctct ctctgatata actttgtgta tttaaattat agccatttat tcgttatgta
     6781 aacatttctt ttttccataa acgtttacct ataaaaaaaa ttttaagtta acgtttttct
     6841 acattcttct attttaataa catactcgct ccaaatctat ataggtcaca ctttaatatc
     6901 aatatactaa ta
//
LOCUS       DAENCV010000170         6901 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAENCV010000170 DAENCV010000000
VERSION     DAENCV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.173"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            43..614
                     /locus_tag="KQD82_003472"
                     /pseudo
     CDS             43..614
                     /locus_tag="KQD82_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(1627..2289)
                     /locus_tag="KQD82_003473"
     CDS             complement(1627..2289)
                     /locus_tag="KQD82_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381914.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2306..4858)
                     /locus_tag="KQD82_003474"
     CDS             complement(2306..4858)
                     /locus_tag="KQD82_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG4381915.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKSDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDYIDEKLNDFVEEGSLDD
                     IKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMKV
                     RVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNNE
                     IQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNLL
                     IRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAYI
                     NYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(5092..5286)
                     /locus_tag="KQD82_003475"
     CDS             complement(5092..5286)
                     /locus_tag="KQD82_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG4381916.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSSDDIERITKEKYTKEN"
     gene            complement(5298..5477)
                     /locus_tag="KQD82_003476"
     CDS             complement(5298..5477)
                     /locus_tag="KQD82_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381917.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEENVEVDNLGDIKIQVKKFDDK
                     TKPLELKLIKEFKLK"
     gene            complement(5482..5739)
                     /locus_tag="KQD82_003477"
     CDS             complement(5482..5739)
                     /locus_tag="KQD82_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381918.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKDKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5999..6901)
                     /locus_tag="KQD82_003478"
                     /pseudo
     CDS             complement(5999..6901)
                     /locus_tag="KQD82_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         2331 a         1044 c          699 g         2827 t
ORIGIN      
        1 aatttattta atcttttata taataataca aggggtaatt aattgaaaaa actagtaaaa
       61 atattaataa atattatttg cattattacg ctaactttct ctagcctttc gatttacata
      121 aaattatctg aatataaaaa gctgatgaag tatatacgga actcagagaa aacactatca
      181 ataatagcaa acatcaagaa ctttatgata aaaataatga ttatagattc tggcttaaaa
      241 taaacaacgt aaatatagac tatcctgtag tacaaggata taacaatgat ttctacctaa
      301 ctcatgattt ttataaaaat tatcttcctt taggtagtat tttcatggat tatagaaata
      361 attttgaaaa tgataagagt ttaatagtct atggtcatta tatgaaaaat aaaactatgt
      421 ttggtcagct agaaaactat actgatgaag tatttttcaa agaaaataat ttagtagaaa
      481 taaattataa agtacaaact tatacttatg aaattttttc agtatatact gctgacttaa
      541 ttaacagaga ttatttaagt atacacttta ataataatga tgagtttaaa tattctttga
      601 attatataac ctaaaaatca gtacacaaat ctgatataaa ggtagatatt tcagataaaa
      661 ttataacttt gtatacttgt aaatatgaat ttaaagatgc tcttactata gttcatgcta
      721 aattaatttc aaaaaagaga cctataagta ggtttgtgta aatgcagact tatctacagt
      781 cttttttgaa cgtaaaattg gtaagtatag aataatattc atattaaact tttgtaggca
      841 aagaaatgaa aaatcaaaga gaaataatta ttacagattt agattaccaa gctgaattaa
      901 gaaagtgtaa aactatggag gatgtagtta gtgaagataa tatataatag tttaacttat
      961 aaattatctt ggtgatatta atgaagtttc aaagtatata ttatttattg atataaataa
     1021 aattaaatct gttgctaata gatgtacctt tgtttggaga caaactattg aagaaatata
     1081 actaaaatgt tgaagtagct acctgacttt attttgaaat gcgaacaaga ttttggcatt
     1141 aagataattt agaaagaatc actggtacac aaggatgtga acttagaatg aatagaagta
     1201 ttcaggcata aggagctttc acccaaataa aacatagtat gaactttaaa agaattctaa
     1261 tcagaggcaa tcaagatatt ctttcagagt ttatacctgt agtgttagcc tatagtattt
     1321 ctaagtttca caaataagat aatcattaat aaatgtagta tgtatattca tcctctcaaa
     1381 actatttttt aatcttacac tttgtaaatt attctatttt aaaatggtat ttttatacct
     1441 ttatttctta tgaccgtttt caagctcaaa ttttgcataa atataaatta atgaaaagca
     1501 agtaaaaaaa gaactgtcgc acagcaattt aaaattactc attcgacagt ccttatttcg
     1561 tggatttaaa tagtggagaa gaatcacaag aataataccc acgattaaac ctttatacaa
     1621 tacattttat atgactttac catcactaat agttattata caatcagcca tcttagctat
     1681 ttcatcatta tgagttatca taaccaacgt ctgcttgtat tcatttacac aatactttag
     1741 taaattcatc acctcattag ttgttttact gtctaaattt ccagtaggtt catctgcaaa
     1801 aattattgat ggcttattgg caagtgatct tgctattgca actctttgtt gttgaccacc
     1861 tgataattca ttaggaaatt tattaatctg actttctaat cctaacttag ttattaaatc
     1921 attaatataa ttattatcta tctttccttt agcaattaaa ataggaagaa ctatattatc
     1981 ataaacattg ataacaggta ttagattata gttttggaat ataaacccaa attctttact
     2041 tctaagatta gatagctttt catcacttaa agtatataaa tctaaattat ccataaatac
     2101 attacctaaa gttggtttat caagccctgc catacagtga agtaatgtgc tttttccaga
     2161 accactttgt ccaattattg cactaaattt atggggttct atctctaaag taattccatc
     2221 aacagccttt atttcatttt ctccttttcc atatattttt ttaatatcta taacttttaa
     2281 catcttcata catttacctc ccaaattatt cattctcttt tataccttca ataatattca
     2341 tactttcaaa atctctatta cttacaacta ctgctattaa acatacaaat acagatatag
     2401 caaagaatag taaagattcc ttaattggga atgtaaaaga cataaaattc tctattccat
     2461 agtttatata cgcatcatta ttccaatttg taaatttaac tagatttata aatgatggaa
     2521 tcatagctat tataaagcta aaaactgcat atgctaatgc ttcatatata agcatttttt
     2581 tcatattttt aagactaaca cctaatgctc taagcgttga aatttctttt tttctaatta
     2641 gtagatttgt tcttatagta cagaaaatat taattgatgc tattattaaa actagtgcaa
     2701 ttattgctat ttgtgattta atgtaacttt tatatatact ttctgacatt tcatgataac
     2761 ttaatctact attaaataga gaccctggta tacttcttga aattttctgt atctcattat
     2821 taacaatctc atcatatgga ttttctaaat ttatgcctaa ttgagtgtat ttttgttctc
     2881 ctgttatgtt tttagagtga atattacttg ttataatttc tagatctggc ataaaatcgc
     2941 catctcccat agcaatccat tcatccttta aaattacagc cactcttacc ttcatctctt
     3001 tataaacaat tgtattattt tcttcaacag gaatttttac agttattatg tctcctatat
     3061 ttaattcttt gaatataggc tttatagtat gatcatcaac tatatcataa aaataattat
     3121 atactgctac atttggatat ccatcagtgt cctttttaat atcatctaag ctaccttctt
     3181 caacaaagtc atttaacttt tcatctatat aatcatcata aagctttaaa actaatttat
     3241 cttccattat ctcttttttt tctactccat tataggttgt aaattctttg tttatatcct
     3301 tattattaaa agtaaacatt ccatttgtta tttgaatttt atgtatactt ttaacaccct
     3361 taattttttc tatattctct acatcactat ccgtgtattt tgatatttgc gggtctgcat
     3421 ttactccaaa ttgcattgtt atattatatc tattttgcat atcttcaatt ttactgttga
     3481 cctcttcttg catacttgag aatgtagtaa tgaatatata tcctcctaaa gcaatagaca
     3541 acatagatat agcagctcta accttatttc ttccaatatt tttaagtccc ataaatccat
     3601 aaaatccaaa tattttgctt actagtctgt ctattccaag tctacttctg tgatttttaa
     3661 ttttgtctgt atttattatt gattcaatag gtgatatctt gccgatttta aaagttggta
     3721 taatacaaga tataaaaaca gaaattattc caaccaatat agcttttata actatttcat
     3781 tatttatata taatttaggg ttaagaccag cttcactgta taaaacttcg ttataattac
     3841 taattcctat atatgaatat gtaaccccag ctactaaccc aagtagtata cctattattg
     3901 ttataattaa tgcttgatag attatcatac accttacgtt tcttttcttt gcacccatca
     3961 gtctaaacag tcccatttct tttactgatt gatttagtgt aatattgaaa atattaaata
     4021 tgaaaattat agatactgta gttattaaaa catttctttt aaaaacattc ggatcactct
     4081 tatcatcttc tacataacga agttccctta caagaggtac atttggtaga aagctttttt
     4141 gtcctaactt atattttttc ataagataat cacattcacc ttcaatattt ctccatccac
     4201 ttttaaaact taatatacta ttataactaa tcgcatcatt aggtataatg ttgtttgttt
     4261 catcattgcc ataagttact gcttcgtatt gccaaaccac atcatagtac ccttttggtt
     4321 tttccataac tcctactatt ttaaattctt tatttataga aaataattga ttacttccgt
     4381 tagtatcttt atagttcttt tttattgtta aatttaatgt agatcctact ccttcactta
     4441 aattcattgc ttctagtgct tttttctcta atactatttc attgttgttt ttaggtagtc
     4501 taccttctat tattttgttg tatctacttt ttagatactc ttcgtttgat gaagttaata
     4561 taaatgagtt tcctttttta tctgaaatac gtcctagatt ttgtaccgaa ttaccctttg
     4621 atactttttc atcagtttct aattttttag catcatcaag tgtcaaatca ctaaactcta
     4681 catggtatcc actgtaaagt tcatgtattg ctactacatt gttttcattt tgtgaatctt
     4741 ttataacact aggtccaaat atcaacataa cacctaacat aactcctatt attaaaagta
     4801 atgttctaag tttatttttc tttaaatatg ctagtgacat ttttaagtaa atccccatat
     4861 aatcctccta ttttactata aattatttat ccaactcatc tgttaagtag attggtggat
     4921 attttaaatc ttttaattct tcattatttt ttgaagctta ctaccatctt cctcatgata
     4981 aacttgcagt atcaccttat ttacatgtag tgcataataa tcaccattat aaaaatatta
     5041 gtatttataa cctggatagt ttttgtaaaa tgatttataa agtatcccat cctaattctc
     5101 ttttgtatac ttttcttttg taatacgctc tatatcatca cttgaaactc cttgatatgt
     5161 catatattta tctatatatt tttcagcttc attccatttt ctaaaatagt tgttgtatat
     5221 aataaatcct attatggcta taatgcttat aattaaagtt aataaaatag ctttgtattt
     5281 tctcatagtg aatcttccta cttcaattta aattctttaa taagttttaa ttctaaaggc
     5341 ttagttttat catcaaattt cttaacttga atttttatat ctcccaaatt atcaacctct
     5401 acattttctt cacctttatc aaatacttct tcaatttttc taaaaaattc tttctcactt
     5461 atttctatat gaatcaatga tctatatgtt ataaatggcc attatgaatt tatcatcttc
     5521 tgacagcttt tcattataag aaaaatcttt aacacattga attgataata tttccttcgt
     5581 atccttcttg tccttagtta ttgaataatg taaaacttgg ctaggttgtt cttttgtata
     5641 ctccaagttt cccaaagaca aaacaaaact tccatctgat ttattttcag ctaccctaag
     5701 accactattt tctattgatt ctgatatttt ctttgacaag tcttcttgat actagaaatc
     5761 tgttcaatag tatatatttt ttcatttgta tctgaaatct tatttgtgca tcctgttatt
     5821 aaacctagtg ttaagcttat agctataacc atagttattg taattttttt atcttcatat
     5881 aatcctcctt atatattgaa aactcgttat ttctcataaa aaaaagacgc tatctttttc
     5941 tattaaaaga taacatccct atatgtttag tatcaatata tgttccccaa ctggtaggtt
     6001 aatcgaccca actatcaaaa ttttgattta atggtatata aatattcaat ataaatttgt
     6061 cttttttatc ttcaaatgat aaatctcctc tatatttttt tatagaagat ttgatgcttt
     6121 aaatccctat tccatgtaca agtttatctt ttttatctgt taataataaa cttttattct
     6181 ttctaattga atttatttta ctattctcac atttaattac gaaataggac tttgagatag
     6241 ttccttttat ttgtatatat ttatcaatac ttttatcatg tatcttatcg caagcttcta
     6301 ttgcattatc taagtatttg caaatatatt agatacatct atttgtttta taaaattcac
     6361 tttggaaaaa ttgatatcac aaatgaattt aatttttctt ttttcactta catcactttt
     6421 ttcatttatt attatatcca atataacatt acctgtattg taaattgatt taaattcttt
     6481 aatttaatct tttaaactat ttacataccc atcaattttc cttttttata tttgagattt
     6541 tctatacaat atatatgatt atttatatca tgataaagct tcttaacttt agcgtgtgat
     6601 tcttgaatac ttagataatg agaatactgc atatctaact tattttttat tacttcattt
     6661 tgcaatttta acttattttc cttaattatt tttataaaca ctataataat tactaagcta
     6721 ctagctagta ttaacctagg tatcacaata tacccaacta tatatatttc ctaataccca
     6781 ccaaatcgat acattttttt atttatataa attattaata tagtaaataa atttgttatt
     6841 aaagcaacag ataagtatat atagtttatt ttattatctt tatctagtcc caatctttta
     6901 t
//
LOCUS       DAENCV010000171         6820 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAENCV010000171 DAENCV010000000
VERSION     DAENCV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.174"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(232..1170)
                     /locus_tag="KQD82_003479"
     CDS             complement(232..1170)
                     /locus_tag="KQD82_003479"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012798.1,HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4381919.1"
                     /translation="MATFEKRSKRIKIEGNINKNNEKLIKGFLQAKKIAGLSEKTIYV
                     YKCDLYTWAKYLHDEMEDLLIEDCTEDDIMEYISFRMEDNHVNRIKVIFSGISSLYDH
                     LRRKRIVTENPVSLIERPKKGLPVVEKHFLNIEQAKELQDKLSKQDDLQLEVYINFAL
                     STAGRVTATSNLRWDNIDFDNRVCENIKEKGGKIVDFYFSEKVKDLLLKLKEVRKKED
                     IVNDYVFLVKYDKEYHKATSNVLRSWAKKAGKLIDIDNLAPHSLRRSFATIAKANNLP
                     LEDISTILNHESTDTTKIYIKEDKGKISKSKDMIGL"
     gene            complement(1205..1516)
                     /locus_tag="KQD82_003480"
     CDS             complement(1205..1516)
                     /locus_tag="KQD82_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381920.1"
                     /translation="MLSKEMVELLSQYGYTAILLMVLMLWLGKYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            complement(1517..1768)
                     /locus_tag="KQD82_003481"
     CDS             complement(1517..1768)
                     /locus_tag="KQD82_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG4381921.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKEK"
     gene            complement(1780..2397)
                     /locus_tag="KQD82_003482"
     CDS             complement(1780..2397)
                     /locus_tag="KQD82_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG4381922.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNFWED
                     GVSAFIDHLALYAGAKGYPKYSELVGKVEYKVNGTTLDPRHFPYLHGEAKTVESLSGK
                     WCPDMNYGQNIINICNKISSMKVENNDVKLEQIKTKVKELNEILG"
     gene            complement(2409..3224)
                     /locus_tag="KQD82_003483"
     CDS             complement(2409..3224)
                     /locus_tag="KQD82_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4381923.1"
                     /translation="MNIKTLTIHAGHNPDNKIGSGAIGNIKESTEARNVLKELLPLAQ
                     KECKVYDCTCNNGTSQSDILNKIIAKCNSYNTDLNVSIHFNSGGGRGVEVLVYNLNDK
                     ETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVDTSD
                     TKKYNAKDMAIDIYEGIFNKSVAGKPQDNKVKYAIVYEGDVDKVIAQLMAMNYKTNEV
                     SVCDLKNYVPGHCENLYVIGGASSKIKTSERFTKLQGDDRWATLHKVLDFIGK"
     gene            complement(3305..3475)
                     /locus_tag="KQD82_003484"
     CDS             complement(3305..3475)
                     /locus_tag="KQD82_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381924.1"
                     /translation="MYSILKRMIEQKNYETREELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTLVEPII"
     gene            complement(3501..3827)
                     /locus_tag="KQD82_003485"
     CDS             complement(3501..3827)
                     /locus_tag="KQD82_003485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381925.1"
                     /translation="MDIEKKIEILIEYGFENDLNNIDNYFIADGKKIFTPVIKNGELI
                     KTGEQVYNEWLELQNNPPKSEPSIEEINADKITILIENQKQQDSLLVDNAYRIAMLEL
                     NTNNVL"
     gene            complement(3842..5743)
                     /locus_tag="KQD82_003486"
     CDS             complement(3842..5743)
                     /locus_tag="KQD82_003486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381926.1"
                     /translation="MIYKDLLKKVNDIENLKEVDNIKCSTDTGEYTIENSKKGYLTNF
                     NVEGKTLIDLWGKTSSDFSLWKATFVDGKINILTENDIRYSNFFTINYTSYKPDTIYT
                     IIVDVDKNTLPSTSGIYIHSLGEENSVFIPNLTNIAIPGGVIGKFKYTFTTISDLNDC
                     NVVLRSVLDNDTLTSGYEISLKITILEGDYTDINIDYSNELLSVGQRDKIEFLSYQYS
                     GINIFNKNADFKDNYILQYLSGEELISEASNHKYTLDYIEIEPDTEYTFYNCSRNICW
                     YDINKSFITASLNERIIGDKDIFYIARSPKNAKYLRVTIIKDLHDNGNKTIITKGNKY
                     DKKTIPYTLRSLPNGIKDEIVYKNDKYYLIKRCEEHTYTDIGNLNLSHVYDNTLQFMG
                     TLTPQAVVDSLDIAYVLCNNLNGKSRNDFNNNDIEGCSTTGSGDIAFKILKSKLTTQD
                     GNGFSEWIKNNPITIIYQLSKPQEIELTSLNLEQYNNQTKFICNTGIVIPDISFESTQ
                     NLGSHIEVIRNNIKNYNVRIDFPFSINFLNGWQPYLGYASSVGNYCTNNNFVTINATI
                     NGGVTTAGTIIGKIPSKYAPRKNIIVIFQTTDGKYYNGIIRTNGEIEIYYNDITYRSW
                     LYLYVNYLI"
     gene            complement(5771..5953)
                     /locus_tag="KQD82_003487"
     CDS             complement(5771..5953)
                     /locus_tag="KQD82_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381927.1"
                     /translation="MNINNIVVRILAERILNGGLNPLKNRPFELDDVTNEDYRKAVED
                     CIIKESGVVERVETTK"
     gene            complement(5953..6246)
                     /locus_tag="KQD82_003488"
     CDS             complement(5953..6246)
                     /locus_tag="KQD82_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381928.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILQHDTITELHCIDVEYGS
                     IDYTKNRIIGINIETKQPILEEIPVFITEEEKRIQELENQILLNENEKVGGIL"
     gene            complement(6263..>6820)
                     /locus_tag="KQD82_003489"
     CDS             complement(6263..>6820)
                     /locus_tag="KQD82_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381929.1"
                     /translation="IINTLALKNIEASLNNTLVELSEKIKASFDSGDASIEDLTNKLT
                     QANNTIGQLNSKYKVASGSSVVNSCVNKYGFYHGSDYLFLFPGAIQINGLNFVPNIFF
                     TTFELIDSGYFRKFFVFACRGIFTQDFVITAHYHRLETYLKDFKVEGEVLKLNERDVY
                     MDNRGIQLPCQRQGSSFKWQAIKFI"
BASE COUNT         2118 a         1175 c          683 g         2844 t
ORIGIN      
        1 atgttatata agtatgtata tatttaataa gaagctattg tagacaaaaa aataaagccc
       61 tttaaagaaa actcatttaa gagtaatcct taaagggctt attatttatc tatctctttt
      121 tcttaccttt tttacgactt ttacatgcca tgatatcact tcttttctac tttatatcta
      181 tttataaacc tatcatatct ttagacttag aaattttgcc tttatatcta tttataaacc
      241 tatcatatct ttagacttag aaattttgcc tttgtcttct tttatataaa tcttagttgt
      301 atcagtggat tcgtgattga ggatagttga tatatcttca agtggtaaat tattcgcttt
      361 agctatagtt gcaaaacttc ttcttaatga atggggagct aagttatcta tatctattaa
      421 tttaccagct ttcttagccc aacttcttaa tacattagat gtagccttat gatattcttt
      481 atcatattta actaagaaaa cataatcatt tactatatct tctttttttc taacttcttt
      541 aagctttagt aataaatctt ttactttctc actaaaataa aaatctacta tttttccacc
      601 tttttcttta atattttcac atactctatt atcaaagtct atattatccc atcttaaatt
      661 acttgtggct gtaactctac cagcagttga tagagcaaaa tttatataaa cctctaattg
      721 taaatcatct tgttttgata acttatcttg tagctcttta gcctgttcta tattcaagaa
      781 atgtttttcc acaacaggta aacccttttt aggtctttcg attagagata ctggattctc
      841 tgtaacaatt ctcttccttc taagatggtc atatagagat gaaatgcctg aaaatattac
      901 ttttattcta ttaacatgat tgtcctccat cctaaatgaa atatattcca ttatatcgtc
      961 ttcagtacaa tcttcgatta ataaatcctc catttcatca tgcagatatt tagcccaagt
     1021 atataagtca catttatata catatatagt tttttcactt aaaccagcta ttttcttagc
     1081 ttgaagaaat cctttaatta acttttcatt atttttattt atgttgcctt ctatcttaat
     1141 tcttttactt cttttttcaa atgttgccaa atacttcact tccttataaa aaagactaga
     1201 gaaattaatc tctagtctct aatttatctt taatttttgt tatgtctgtt tcaactcttt
     1261 caagtctact gttattagta tttaatgctt cagcaaattt atctaattta tcatcaaact
     1321 tatctattgt attaccaaat aaagttctct gctctttaat ttctcttgaa aaataagctc
     1381 tttccttttt tctatcatct tgttctagtt gtctattttt ctctaaatat tttccaagcc
     1441 ataacatcaa taccattaaa agtatggctg tatacccata ctgacttaat aattcaacca
     1501 tttctttaga taacatctac ttttcctttc tgttctgtac ttgtatttga tttattccta
     1561 tagatgcccc ccaacataaa ataccttcaa aaacagcttc tattgacagc tctttcataa
     1621 acaaacaact acttatcatt gctataacta acaatataaa aggtatgtat ttatttttaa
     1681 tattagattc ttttataaat gttcctaata tgtaaagccc acctattaac attattaaat
     1741 tttcaggtat aaattgcatt atatccatgt gatttcctcc tatccaagaa tctcatttaa
     1801 ttcttttact ttagttttta tttgctctaa ttttacatca ttgttttcta ccttcattga
     1861 acttatctta ttacatatat ttattatgtt ttggccataa ttcatatctg gacaccattt
     1921 gcctgataaa ctttctactg ttttagcttc tccatgtaaa tatggaaaat gtctagggtc
     1981 taatgtagtt ccatttactt tatattcaac tttaccaact aattcactat attttggata
     2041 tcccttagct ccagcatata atgctagatg gtcaataaat gctgatactc catcctccca
     2101 aaaattaaat cttgtatgag ctgttgggtc taaatctcct ccacctttat ttcctttaag
     2161 accacacaca ttgcaataag aaggatttaa aactctacca aaattaaaat atccagtttc
     2221 tacaaatgct tgagcaattg caataactgg atttacacct tttttctctg cttctttata
     2281 tataattggt actatagctc tgcataaaaa atgtggtgga ttagagttat tttttaatgt
     2341 gtctaaatac gaaaagactt gttctaatgt agctgttgat ttatctaata tattcaattc
     2401 attacctcct acttgcctat aaaatctaac actttatgaa gtgtagccca tctatcatcg
     2461 ccctgtaact tagtgaatct ctcgctagtt ttaattttac tggatgctcc accaatcaca
     2521 tataggtttt cacaatgtcc aggaacataa tttttcaaat cacacacaga gacttcatta
     2581 gtcttataat tcatggccat taattgggct ataactttat ctacatcacc ctcataaact
     2641 attgcatatt tcactttgtt atcttgtggt ttaccagcta cagacttatt aaatattcct
     2701 tcataaatat ctatagccat atctttagca ttatattttt tagtgtcaga tgtgtctaca
     2761 aaacagcact caactaaaat tgactttgcc ttcgttcttc ttaaaaatgc tagagttttc
     2821 ttttctttaa ctccacgatt tttaaaatct ttatcacctt ttgcatgata agtttcagtt
     2881 atttttttac atattcttga tgctatttca acagtttctt tgtcatttaa attataaact
     2941 aaaacttcta ctcctcgacc accaccactg ttgaaatgga tacttacatt tagatcagta
     3001 ttatatgaat tacatttagc tataatttta ttcaatatat cactttgaga agttccattg
     3061 ttacaagtac agtcataaac tttacattcc ttttgagcta aaggtaataa ctcttttagt
     3121 acatttcttg cttctgtaga ttcctttata tttcctattg ctccagaacc tatcttatta
     3181 tctggattat gtcctgcatg aattgttaat gtttttatat tcaaaattac cactcctttt
     3241 aattaaaaga gaattgaaat taatcaatcc tctaaaattg acataaaaaa agaaccttta
     3301 tactttagat aataggttct actagtgtat cttctttatt taataaattt gttaactctg
     3361 tgtattcact ttcctttatc ctgttcatag cgtaaaatac atctaactta gtctgcaatt
     3421 cttctctagt ttcataattc ttttgttcaa tcattctttt taaaatacta tacatgttgt
     3481 ttcctccatt ttttatagtt ttataacacg ttatttgtgt taagttctaa catagcaatt
     3541 ctataagcat tatctactaa taaactgtct tgttgctttt gattttctat taaaattgta
     3601 attttatccg catttatttc ttctatactt ggctctgatt taggtggatt attttgtaat
     3661 tctaaccact cattataaac ctgttctcct gttttaatta gctctccgtt ttttatcact
     3721 ggagtaaata tttttttacc atctgcaata aagtaattat ctatattatt taaatcattc
     3781 tcaaatccat attctattaa aatttctatt ttcttttcta tatccatctt atttttcctt
     3841 tctaaattaa atagtttaca tataaataca accaactacg ataagttata tcattataat
     3901 atatctcaat ttctccattt gtacgaatga ttccgttata atactttcca tctgttgttt
     3961 gaaatattac tattatgttt ttacgaggtg catattttga aggtatcttt ccaatgatag
     4021 tacctgccgt tgtaacacct ccattaatag ttgcattaat tgttacaaag ttattatttg
     4081 tgcaatagtt tccaacagaa ctagcatatc caagatatgg ctgccaccca ttaagaaagt
     4141 ttattgaaaa tggaaaatca attctaacat tgtaattttt tatattattt ctgataactt
     4201 ctatatgact tcctaaattt tgtgtacttt caaaactaat gtctggtatc acaatacctg
     4261 tgttgcaaat aaatttagtt tgattattat attgttctaa atttagtgat gttaattcaa
     4321 tttcttgtgg ttttgaaagt tgatagataa tagttattgg attatttttt atccattcac
     4381 taaacccatt tccatcttgt gtagtaagtt tactttttaa tattttaaat gctatatcac
     4441 cactaccagt agttgagcat ccctctatgt cattattgtt aaaatcattt ctactttttc
     4501 catttaaatt attgcataaa acatatgcta tgtctaaact atctacaaca gcttgaggag
     4561 ttaatgttcc cataaattgc agagtattat catatacatg tgataaatta agatttccaa
     4621 tatcagtata agtatgttcc tcacatcttt ttattaagta atatttatca tttttataaa
     4681 ctatttcatc ttttattcca tttggtaaac ttcttaatgt atatggaatt gtctttttat
     4741 catatttatt tcctttagtt ataattgttt tattcccatt atcatgtaaa tcttttatta
     4801 tagtaactct tagatacttt gcatttttag ggcttctagc aatataaaaa atatctttat
     4861 ctcctattat tctttcgttt aatgatgctg ttataaaact tttatttata tcataccaac
     4921 aaatatttct actacaatta taaaaagtat actctgtatc tggttctatt tctatatagt
     4981 ctagggtata tttatgatta ctagcttcag aaataagttc ctctccacta agatattgta
     5041 aaatataatt atctttaaaa tcggcattct tattaaagat attaattcca ctatattgat
     5101 aacttaaaaa ttctatttta tctctttgcc caacacttaa tagttcatta gagtaatcta
     5161 tattaatatc tgtataatct ccttctaata tggtaatttt caaacttatt tcataaccag
     5221 atgtaagtgt atcgttatct aaaacactcc ttaaaacaac attacaatca tttaagtctg
     5281 atattgttgt aaatgtatat ttaaatttac caattactcc tcctggtata gctatatttg
     5341 tcagattagg aataaatact gaattttctt cgcctaagct atgaatatat attccacttg
     5401 tactcggtaa agtgttttta tcaacatcaa ctatgattgt atatatggta tctggtttat
     5461 atgaagtgta attgattgta aaaaaattag aatatcttat atcattttct gttagtatat
     5521 ttatttttcc atccacaaaa gttgctttcc ataaagaaaa atctgaacta gtttttcccc
     5581 ataaatcaat taatgtcttt ccttctacat taaaattagt taaatagccc tttttactat
     5641 tttctatagt atattctcct gtatcagtac tacattttat attgtcaacc tccttgagat
     5701 tttctatatc attgaccttt ttaagtaaat ctttatatat caaattaaaa cacctctctt
     5761 cacaaaaccc ctattttgta gtttctactc tttctactac tccactttct ttaattatac
     5821 aatcctctac tgcttttcta taatcctcgt tagttacatc atctaattca aaaggtctat
     5881 tttttaaagg attcaatcct ccatttaaaa tcctctctgc taatattctt accacaatat
     5941 tatttatatt cattataaaa ttcctcctac tttttcattt tcatttaata aaatttgatt
     6001 ttctaactct tgtattctct tttcttcttc tgttataaag actggtattt cttccaagat
     6061 aggttgttta gtttctatgt ttataccaat aattctattt ttagtatagt ctatacttcc
     6121 atactcaaca tcaatacaat gtaattcagt tattgtatca tgttgcaata tatctcctgt
     6181 tgcttctcct gtttgcaaga gtattttgcc tgtttggtca catataattc tatttgctct
     6241 gttcacttta tcacctcatt tattaaataa attttatagc ttgccactta aaagaagaac
     6301 cttgtctttg gcaaggaagt tgtatgcctc tattatccat ataaacatca cgttcattta
     6361 atttaagaac ttcgccttca actttaaaat cttttaaata tgtttctaag cggtgataat
     6421 gtgcagtaat tacaaaatcc tgagtgaaaa taccacgaca agcaaaaaca aaaaatttac
     6481 gaaaataacc gctatcaatt aattcaaaag tagtaaaaaa tatattagga acaaaattaa
     6541 gtccgttaat ttgaatggct ccaggaaata ggaatagata atcgcttcca tgataaaacc
     6601 catatttatt aacacaacta ttaacaacac tactaccact agctacttta tatttagaat
     6661 ttaactgacc tatagtgtta ttagcctgtg ttaatttgtt tgttaaatcc tcgatactag
     6721 catctccact atcaaaactt gctttaattt tttctgataa ttcaactaat gtattattta
     6781 aacttgcttc tatattcttt aatgctaaag tatttataat
//
LOCUS       DAENCV010000172         6783 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAENCV010000172 DAENCV010000000
VERSION     DAENCV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.175"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(284..1174)
                     /gene="metA"
                     /locus_tag="KQD82_003490"
     CDS             complement(284..1174)
                     /gene="metA"
                     /locus_tag="KQD82_003490"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG4381930.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1204..2469)
                     /locus_tag="KQD82_003491"
     CDS             complement(1204..2469)
                     /locus_tag="KQD82_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG4381931.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2579..2681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2735..5392)
                     /locus_tag="KQD82_003492"
     CDS             complement(2735..5392)
                     /locus_tag="KQD82_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG4381932.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5520..6263)
                     /gene="yaaA"
                     /locus_tag="KQD82_003493"
     CDS             complement(5520..6263)
                     /gene="yaaA"
                     /locus_tag="KQD82_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG4381933.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6273..6737)
                     /gene="bcp"
                     /locus_tag="KQD82_003494"
     CDS             complement(6273..6737)
                     /gene="bcp"
                     /locus_tag="KQD82_003494"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG4381934.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         2090 a         1243 c          801 g         2649 t
ORIGIN      
        1 aaatctatat atatatttta cactgatttc tcccttttta atctctttta aaagttaaaa
       61 tacaaattta ttgatactat aaaggtttag tctcccatta tcgcaccata tataaaatta
      121 aagccacctc tatctacata cacctaaagt gattttctta aaatataaaa ctactattat
      181 aaggtcaaat tttaaaaaaa taaggtataa agtatcatta agattaactt tatacctata
      241 aatttctata tttttttagt taacatttat atactcttaa aatttattgt atcaaatagt
      301 tttttatcca atttctaaac aacaattctg aatgtttttt ccattttaca gttggctctt
      361 ctgaagggtc atcattaata tagtaatttt gaggtataga tattttatct cctttattta
      421 tatccctctt atattcttta tcaagagtgt atttgtcata ctcgctatga cccattacga
      481 acacatctct tgaatttgta attatatagg gaccagcttc tttagaatga ctcacaatgc
      541 tcaactcttt attctctttc aaagcttcaa tatttacagt agtatgtctt gaatgtggag
      601 caaaaaattc attttcaaat ccatctacta ttttactatc tttatctacc ttatgcttaa
      661 atactccaaa acatttttga cttagtggaa gtttttttac attgtagtat ttgtaaagac
      721 ttgcttgagc tgcccaacat atatataatg tagatttagt ttttctctta gaataatcca
      781 taatttcttc tagttcattc caataatcaa cttcttcaaa ctccatttgt tcaactggag
      841 ctcctgttat tataaagcca tcaaaatact ctcctttggc ttcatcaaat gttttataaa
      901 attctttaac atattcatta caacttcttt tactttctct tgttgaaatt ttaataaatt
      961 ccacagacac gttgaatcct gttttatcta tccttcttag taaatctaat tctgtatcta
     1021 tctttatagg cattagattt agtatagcta tcttagtgtc tatgttttct tcatatttta
     1081 attccttttt aaaatcttct ttataaatta catctattcc ttcatctaat aatttattaa
     1141 taacaggtaa tccctttggt agtattaaag ccatatcttt tcacttctct ttctcttaga
     1201 atattagaaa tttaaagctt gatttaaatc agctattaaa tcctctacat tttctattcc
     1261 aacagataat cttattagtg aaggcaatac tccagatgca attaattgtt cttctgttaa
     1321 ttgtctatga gtagttgaag ctggatgtat aacacaagtt cttacatctg aaacatgtgt
     1381 aaccaaagat gctatctgta atttttccac aaattttttg gcattttcta acccacctct
     1441 tactccaaat gataaaacac cactacatcc tcttgataaa tactttttgg cattctcata
     1501 atacttgtta tcttcaagtc ctgggtaatt aatccaatct acattttcat gtttttctaa
     1561 aaatctagct attttaagtg cattttcact atgtctctcc attctaagat gtagtgtttc
     1621 aacatttaaa ttagtaagat atgcattgaa tgggcttaaa caatttccat agtctctaag
     1681 caactgaact cttgctttag ttgcatatgc ggcatttcca aatttttgag tatagcttat
     1741 accatgatat gttgggtctg gctcaactaa ttctggaaat tttccattat cccagttaaa
     1801 gtttccacca tccactataa ttcctccaac acttgaagca tgcccatcta aatattttgt
     1861 ggtagaatga gtaactatat ttgctccata ctttaaaggg ttacaaagca ctggagatgc
     1921 taatgaatta tcaacaataa atggaacatt aattctctta gctacatttg ctattttttc
     1981 aaaatctatg acttcaagag ttggatttgc aagtgtttct gcaaacacaa ccttagtatt
     2041 ttcctttgca agttctacta tctcatcttc acttgaatct aagtcaaacg atattaaatc
     2101 tataccaaat tttttaagac taggtcctag taaattgaat gttcctccat atacttttga
     2161 agaacaaagt atactatctc ctgatttaca tatatttaaa actgccaaca tatttgcaga
     2221 ttgccctgat gatacagcta cagaagatac tccaccctct agtaaactta ttttttcttc
     2281 aaaagcttga atagtaggat tgcttattct tgaatatata tgtccatcaa cttttaaatc
     2341 aaataattca gcaagttggt ctatactgct atatttaaaa gttgtacttt gatataaagg
     2401 tagtactctt ggttctccat tacctggttt ataatttcct tgcacacata ttgtttcttt
     2461 attatacatt cttgtatccc ccttagattt tagtaaaaaa aattaaacta tttaccagat
     2521 gagagttaaa ataaataaag cctgagtata taactcaggc tctgtataag cttaattata
     2581 ctcatctctc agctttcgcc gcaggattta gcaccaatgt atataaatat actggttgcc
     2641 gggtttcata gggccagtcc ctccacctct ctggataaga tttatttaat ttttctatgt
     2701 tttatatctt atatattttt cctaattttg tcaactattt ttttaatttt tttcttttaa
     2761 taattttccc aacctctact tgaattacag acattatggc taaaacaatc gttataagcc
     2821 actctaagtt atttaaaact gttaagttaa aagcatttct aaaaacaggt acaaatagta
     2881 ttactgctat taaaaatgca gaagttaaaa atgagattaa aagaaatgga tttttaccat
     2941 tatttctatt aaacattgaa tctgtatttg aacgctgact aaatgctcgt aacatctgtg
     3001 aaaatgctaa tacacaaaat gccattgttt gacctacaga atgattttct atattaagac
     3061 caattttata tgctaaaata gtcattattg ccacaaatat accttgggta acaactcttt
     3121 gtataagatt tttttcaaac aaagtggttg atttcactgg ctggtgcttc attatatttc
     3181 tgcttggtgg gtcaactcca agagcaaggg ctggaagcgt agcagttgca aggtttaccc
     3241 atagaatatg aacagcaaga agcggagtat cccaatttaa taatattgct acaaacaacg
     3301 taagtatctc tgctatatta ccaacaagca aaaattgtat aactttttgt atgtttctat
     3361 atacacgtct accttctttt attgcataag caattgttgt aaaactatcg tccattaaaa
     3421 tcatgtcaga agaatctttt gcaacatctg taccacttat acccatggct ataccaatat
     3481 cagcagactt taaggcagga gagtcattta caccatcccc agtcattgct ggtacttctc
     3541 caattctttt tagagattgt ataattctaa gtttatcaaa tggggataca cgagcaaata
     3601 ctgtagtatt tttaacagct tcatctagtt catcatcact caaattatca agttcatcac
     3661 cagatatcac agtattttct tcactccata ttcctaactc atgagcgatt gttgtggcag
     3721 taactttatg gtcacctgtt atcattatgg ttcttatacc tgcttcttta caagtactta
     3781 ctgcatcaat tacctcagtt cttggtgggt caatcatacc aaccatacct ataaatgtta
     3841 aatcatattc tatattttca ctatcttctt ttggtataga tgaaatctca cgcttagcaa
     3901 aaccaagtac acgcaatgca tctttagaca tgttaagaca taattcgtgt atattctttt
     3961 tatccacttc ggtaatgttt ctttcacctt ttgaagttaa tattttagta cataggtcaa
     4021 gaatttcgtc tactgctcct ttagtataag caataatctt ttcatctata ttatgaacag
     4081 ttgtcataca ttttctatct gaatcaaaag gctgttcaaa tacacgtgga tattcttctt
     4141 caagagcttc ttgatctatt ccaaattttt tggccataaa aattaatgcg ccctctgttg
     4201 ggtcacctaa tatttcatct gttttatctg gattaataca tgcatcatta caaagtgcac
     4261 cagcataaac aatctcttta tacactaatg gatgtttttc agatgcagta tctattgatg
     4321 tcacttttcc agattcaaaa tctccattga cagcaatatg tgtaacagtc attttattta
     4381 aggttaatgt accagtttta tcacaacata ttactgtagc tcctcctaat gtttcaacag
     4441 caggtaattt tctaattaaa gcatttcttt tagccatacg ctgtacgcct aaagccatta
     4501 caatagtagc tgttgcaggt aatccctctg gaataattga tatggcaagg gaaattgcaa
     4561 tcataaacat tggcaagaga ggtctctgat aaaatgcacc tattgcaaat ataacaacgc
     4621 atacaataat acctatgaca cttaatgtct ttccaacagt attaagttta cgttttatag
     4681 gagtatcaaa atcatcttgt gaatctaaaa gatgtgctat gttacccact tctgtatcca
     4741 taccagttgc tactacaaca cctacacctc taccatatgt gactattgaa gatgtatacg
     4801 ccatattgct acggtctcct aatacacatt ctttaggtaa gattgtatct gcatcttttt
     4861 ctgatggaac agattctcct gttaaagaag cctcttgaat ttttaaactt gatgtttcta
     4921 ttaaacgaag gtctgctgga accatagctc catcttctaa aaaaacaata tctcctacta
     4981 caagattact tgctggaata acattttctt caccatcacg cataacacga gctgtaggtg
     5041 aattcatatt ttttaatgct tctaatgatg attctgcttt tttctcttga acaattccaa
     5101 taactgcatt tactgttaca attattagta ttacaaaagc ttctgtaaat tcgccaaata
     5161 tcaaagataa tattgctgct cctattagta tcataaccat agcatctgta atttgactcc
     5221 aaagcatttg taaagttgtt ctaggtggtt tttttctaag ttcatttaag ccattctctt
     5281 taagccgttt ttccgcctcg gtatcggtga gcccctcctc tgatgaatct agactttcta
     5341 aaatttcatg cgttgctctt gaatgccaag gcatctgttt gtgattttcc attttggaag
     5401 accactcctt tcttttttta atttaagaat aattatattt taaaatcaag ttcaattcaa
     5461 tacacactaa gtatatttta cctaaattta acaatgccat ttaatattta acaaaacttc
     5521 tactctttta caaatatgta ttcactattg ttagataagt tttctttata cttataacct
     5581 tctaaatcaa actttttcag ttcttcaata tctcttatcc tattttttac aatgtatttt
     5641 gtcatcatac ctctagcttt ctttgcttgt gtagattcca ctttcaatat gccattttta
     5701 tttactttaa aagtacatgt aatatatgta tcactatctt taataaattt ttctatggat
     5761 ttgctgtatt catttgatga aagattaacg atagctgttt tttctttgat tagttcttta
     5821 tatatacgat ttccccaata ttcgtataaa ttcttaaact cacctacttg taattttgtc
     5881 tgcatttcta atctatattc atatatacta tcatatggtt ttacaacacc atacaatcca
     5941 cttatgattc gaatacagct atttgcaaac tcttcatctt tccttgtaaa attttctgct
     6001 tctatatttt tatattgtat tccatcataa gttaagattg cagggtttcc taatctatca
     6061 aatttaatgc tttcaaatct atttttattt aatacagcta atttttcatt aattttcatt
     6121 ttatttttaa aatcttctat accaaatgtc tttatatttt ccactatttc ttttgtgtta
     6181 tctataaagc aaggcaaact caaatctcta tcaaatactt ctatattctt catattttta
     6241 gctggtgata acattataat cataaattct tattactcct gtttttccaa atattcaaga
     6301 atctcttgtg catttgtgtc tggttttgct ttttcaaaga ctttttcaat aattccattt
     6361 tcatctataa tatatgtcgc acgtacaact cccaatgcag tttttccata catttttttc
     6421 tctttccata catcaaaagc ttttatagca actaagtcag ggtcagaaag taaaataaaa
     6481 ggaagttcat gcttttctgc aaatttttga tgtgatttta tactatcttt acttatccca
     6541 ataacttgta tatcttcctt tttaaatcca tcatatgcat ttctaaaagc acaagcttga
     6601 cgtgtacatc ctggtgtatt atctttagga taaaaatata ctactacttt tttaccttta
     6661 aaatctgaca tacttacctt atttccatct ttatcttcta gtgtaaattc tggtgctttt
     6721 gttcctatac tcaacataaa tatctctcct tttgttttta ttaataattt tatactatgc
     6781 tta
//
LOCUS       DAENCV010000173         6686 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.176, whole genome shotgun sequence.
ACCESSION   DAENCV010000173 DAENCV010000000
VERSION     DAENCV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.176"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            19..219
                     /locus_tag="KQD82_003495"
     CDS             19..219
                     /locus_tag="KQD82_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG4381935.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            854..1666
                     /gene="modA"
                     /locus_tag="KQD82_003496"
     CDS             854..1666
                     /gene="modA"
                     /locus_tag="KQD82_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4381936.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1676..2467
                     /locus_tag="KQD82_003497"
     CDS             1676..2467
                     /locus_tag="KQD82_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381937.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            2469..3164
                     /locus_tag="KQD82_003498"
     CDS             2469..3164
                     /locus_tag="KQD82_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4381938.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            3151..3882
                     /locus_tag="KQD82_003499"
     CDS             3151..3882
                     /locus_tag="KQD82_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381939.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            4196..4996
                     /locus_tag="KQD82_003500"
     CDS             4196..4996
                     /locus_tag="KQD82_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG4381940.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            5535..6251
                     /locus_tag="KQD82_003501"
     CDS             5535..6251
                     /locus_tag="KQD82_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381941.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2626 a          798 c          922 g         2340 t
ORIGIN      
        1 ttaataggag tatttttaat gaataatgga atagtaaaat ggtttaataa tgaaaaagga
       61 tttggattta tatcaatgga aagtggagat gacgtatttg ctcatttctc agctatacaa
      121 acttcaggat ttaaatcatt agaagaaggt caaaaagtaa gctttgatat agttaaaggc
      181 gctagaggtc ctcaagcaga aaatataact atattataat atatagattt ttcggaaaga
      241 ctcttttaag agtctttttt ttatttacgc attttgtaaa aaaggaaaaa ataacttcaa
      301 caaaaaaata cttaaaaata atacttagtc taaatacttt ttataaaata aatctgacaa
      361 aaatatattt ataaagtatg tacttaaacc tacaagtact cttttaatta gaatatatag
      421 taatataata attttagttg tggcacatca aaattttcaa ctatctcata aatacatact
      481 ctttcttttg tattcaattc tgatataata cttttagtaa tttgttaata aaaagcaatt
      541 aaaacatatc tatatgtttc aatgttctat tttttcactg aaatataata aatataaaat
      601 tagttaaaaa gtaaaaaatc tacaataagt taatttgatt taagttttta aatttaaaat
      661 actttaaagt attaataaaa attaaaaata aaataactac ctatggaaat gacattatga
      721 atagattttt tcttaactat acataacaaa acataacaaa acattataaa acacaacaaa
      781 aacttagaca tcgttttctt agtatgatat actaaaaaag aatttacact aatttaataa
      841 gggggaatat cttatgttta aaaaactttc agtactagct ttagctatga ttatttcatt
      901 aagtattact gcatgtaatt ctaaagaaaa caaagctaat gatgaatcta atactaaagt
      961 gacaacagct gatgattcaa aagaaaaaga gactcttttt gtctattcag gggcaggttt
     1021 aaagaaacca atggatgaaa tagcaaaaaa atttgagaaa gaaaataatg tcaaagttga
     1081 atatagctat gcaggttctg cacagctaat agctcaaatt gagacaagtc acaaaggaga
     1141 cgtatttatt gttggttctg aaccaattta ttcaaaggcc tttgaaaaag aacttgtagg
     1201 tgaacataaa acggttgcac atcatacacc tgcaatagtt gtacctaaag gaaatccagc
     1261 taatattcaa aaattggaag acttaaataa agatggagtt aaacttattt tgggagacaa
     1321 agagtctaat gctataggta agactacaca aaaaatactt gaaaaaaata atttaaaatc
     1381 tataaatgat aatgttgtct ctacagctgc aactgtaaat gaaatgattg tacagttaac
     1441 atcaagtaaa gctgatgcta caatagccac aaaagattcg gtctttggta atgaagatgt
     1501 agaagttata gaaattgccc aagataaaaa tatagaccaa ctcatatcag caggaatcgt
     1561 aaactatagt gataaaaaag atttagctaa taaatttata aattatatag agtcagacga
     1621 agctaaacag atatttaaaa aatatggttt tgaacctgtt aagtagtgat taaacttgaa
     1681 aaataaattt tttactactc tacttactgg attttttgct atagtctttt tgtttattgt
     1741 atcaccactt cttatgttaa tcattaaagg gataagttat gttcctatat gcttaaaatc
     1801 agtggaagta cagtatgcaa taacacttag tattaaaaca tctttaatat ctactatagt
     1861 atgcctttta ttagctattc cagtagcata ttttttacat attactaaat taccatttaa
     1921 aaaactgatt atacagataa taaatctccc tatgtctctt ccacacttag tatcaggaat
     1981 tgctctttta cttttgtttg gaagaatggg gattggagat tccatttata agatttttaa
     2041 gcttgatttt atatttacaa agcaaggtat agttttagca caaatatttg taaatcttcc
     2101 acttactatt aaaatacttc acacttcatt aaatgaaagt aatgaaaaga tgatatttgt
     2161 tgcacgtacc cttggatgta atagttggga agcatttaga tttataattc ttccaaatct
     2221 aaaaactgga atcatatctg caactgtaat gacttggtca agagcacttg gtgaatttgg
     2281 tgcagtagct atgatagcag gttctacacg tatgaaaact gaaattattc ctacatctat
     2341 atatttaaat atgtctactg gtgatataga cattgctatc ggaatagctg taattttgat
     2401 atttctctca cttacatgct tgatgctttt tgagatattt tttaatagag aggttgataa
     2461 gaattaatat gttaaaaata aaaaatttat ctcttgattt acctaatttt aaattacaag
     2521 aaaatagttt aaatattaat aatcatgaat actttgtttt attaggagct agtgggtcag
     2581 gtaaaactct atttctagaa acattagcag gaagatatcc atctgctaaa ggaaatgtat
     2641 catacaaaga aaatttaata tcaaacttac ctccagaaaa tagaaatata ggatttgtat
     2701 atcaaaattt tgaattattt ccaaatatga atgttgaaga taatataagg tttccactta
     2761 aattgagaaa aatacctata gatgaacaga ttcttaaggc taaccacttg tctaaattaa
     2821 tgcagataga agatataaaa aataggtatc ctaaaaattt aagtggaggc gaaaagcaac
     2881 gagttgcttt tgcacgtgcc ttaatagctg aacctgatat attactttta gatgaaccta
     2941 tgagttcatt ggattatatt acaaaaaaac atataagcaa aattttgaag gatgtctaca
     3001 taaaatacaa accaacagtg attcatgtta ctcatgatat ttctgaagca atgttttttg
     3061 ctcataagat aggaataatg aacaatggaa aaataaatca tatttttgat ttaaatgaag
     3121 aaataaaaaa taaaggagaa tcttttttct atgaatatat ttgatatttt gagagattat
     3181 ctattagtac aagtcgataa atataattta aatatggata tgataagtat tgtgtccaaa
     3241 tctctatctt caaaagaagc aattggaaat actaagcgta aagatttccc aattatagtt
     3301 ggtaaagaaa taatgcttga agctgacttt aaaggagcta aaggacaagc atttacttct
     3361 actccatcta catttgaagg ttctttaaaa gacatattat cattagattt acatgacaat
     3421 tctcatgata gaagtctttt tatagcatca ttaaatgcag ttatgaaata cttaggcaag
     3481 acggatagaa ccattcattg taaaaataac gagccagagg tatgtgcaaa aaaattccct
     3541 gagtttataa aaatggaatt tggtaatcca aaagtagcaa ttataggata tcaaccagcc
     3601 ataattgata atattaagga ctttttcgaa acaagagtat tagatttaaa tcctgaattt
     3661 gtggatacta ttcaatataa tgttaagatt gaagatggta tacgagatta tgaagatgta
     3721 atatcttggg ctgatttagt gatttgcaca ggaagtacat tatgtaataa ctcaattata
     3781 aattttctat cacttaacaa accagtttac tattatggaa ctacgatagc tggtgcatca
     3841 aatattttag gtttaaaaag actctgtttt tgtagtaaat aagcaatcat atcatcaagg
     3901 agtacacaaa tatatatgtg tactctttaa atttaaaaag ttattacttt tattctaagt
     3961 actaaagtaa aattctatta aaatgaatcc cgaataaatg tacaatccct ttaaatctca
     4021 attcctatca atcattaata ttttgattag tcttatacat aaatgttttc tttattttaa
     4081 actaataaaa aaattttatt ccattaatct actctatatt tttcagcatt taagaaataa
     4141 tttttagtac tttgtttgat aatataacta agccaacctt tcaggagatg attaaatgac
     4201 atttaagaat aaaaaattta ctataaataa atataaaatt ctatctatta ttctagtttt
     4261 atgtttgatt ttaaccgctt ttagtagcta tgtgaattat agtcataaaa aatttaaaga
     4321 ttatgttgtc ttctatgttc agcatcaaga tgatgaagtg ttgtgggctg ggagtgctat
     4381 tgttaatgct ataaaagaaa gaggaaaaaa tcatgtattt gtggttttag tatctactgg
     4441 atgtggtata tccgtctttg ataaatataa aaaatatgaa aacctaacaa atatacaaaa
     4501 atcagaatat agaaatagag aatttttagc ttctcttaat agccttggtg taataaggga
     4561 aaatataata ttattaccag aaacaaatac aaatggtaat actgacttca attacatgga
     4621 aaaaattgct cttacatttg aagaaaaatt taaaaatgta actcatatag cacatactta
     4681 taaattagat gaccatttac aacatttaaa aaatggttct gttatccaaa atttatataa
     4741 tgctggtaaa ataaaagatg ctaaatattt tgtaaaacct aaatttgctg ataaaataac
     4801 ttttaataat aagatagttt ataaagctca ttctactgag gactatgaaa aggttaaaaa
     4861 tgcctgtcaa caatacaaaa ttgttgatga attagaatat agagaaggta ttggctataa
     4921 gtctgaccat aaatcatttg ataagttatt aaataataaa gatgttcctg ctatacttca
     4981 tacacctaat ttataagaaa tatttattct ttaatttaag ttataaaaag gaaattattt
     5041 gagattttgt gatatagcac tttttgtgct atactacttt ttctcataca actattaccc
     5101 atatctaatt tcatcatctc ttaaaatata taaagatatc atcattaaaa cctataagcc
     5161 ttattaaatg ctttatacat acctacatta aactacctgt ttatattaaa ctacattaaa
     5221 ataaatattt gttataaatt cttaataatt atgaaactca tttgttattt atatttgtta
     5281 atatatgttt aaacctaaaa aattaataaa ccaaaataac ataagaagtg tgatagatta
     5341 ccctagatat tttcataaac ttttaccttc agattaatat gggcgtctat acttttaaat
     5401 ttactatatg cattgacaat atattaaaag tactagtttt tatttcataa tgaacgggct
     5461 tactgttttc taatacctct ttttggtata aaataaaaat tggttaaaaa taaaatttca
     5521 agggggttaa taaattgaat caaaatatat tggttgtaga tgatgataga gagatagttg
     5581 atgctataga gttctatttg agacctgata actttaatgt tttaaaggca tatgatggac
     5641 ttgaagcatt ggacatacta atcgaaaatg atattaaatt aataataatg gatgtaatga
     5701 tgcccaattt agatgggctt aaaactacac ttaaaataag agaaaaaagg aatataccaa
     5761 taattctctt atctgcaaaa tcagaagata tggacaaaat tataggtctt aatatgggtg
     5821 ctgatgacta tataactaaa ccatttaatc cactagaact tactgctcga gtaaaatctc
     5881 aacttagaag gtatataaat ttaggtgatt ttagtaatac aaataactca aatgatacta
     5941 attatattct aaaaagtggt ggcctagagc taaatacaga tacaaaaata gtttctttag
     6001 atggtgagga aattaaaatg acaccacttg aatataaaat acttacactt cttatgtcac
     6061 gaaaaggtaa aattttctct tctcatgaga tttatgaaaa tgtctggaat gaggatgctt
     6121 atagttgtga gagaactatt gctgttcata ttagaagaat tagagaaaag attgaaatta
     6181 atccaaaaga accaaaatat ttaaaggtgg tgtggggcat tggttacaaa attgaaaaac
     6241 ttaattatta atgattctaa agcaaaattt attttattat tattactgtt tctattagtt
     6301 tctatattat ttgaagcagt ttatgatttg aataataact tttctaatat taattattat
     6361 tatagagagt ttgctctccc tcatttttat tttttcggta ctcctgtaca tttgtcaaaa
     6421 gtagaattag agtttgtctg cataggtatt atattatcaa gttttttatt gactatacta
     6481 tgttttacat tgtataaaaa aaataaacaa actaaagtca aattcttaga gaatgtcata
     6541 aacctttttt ctaaggtaaa aatagaactt acacttatat taactttgat actattaacc
     6601 ttaaggtttc atgcatttac aagtaataaa atgttttaca acactaattt aataatctac
     6661 aattttataa ttatatctat agtcta
//
LOCUS       DAENCV010000174         6642 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAENCV010000174 DAENCV010000000
VERSION     DAENCV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.177"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     misc_binding    361..599
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            663..2582
                     /gene="thrS"
                     /locus_tag="KQD82_003502"
     CDS             663..2582
                     /gene="thrS"
                     /locus_tag="KQD82_003502"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG4381942.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3350..4441
                     /locus_tag="KQD82_003503"
     CDS             3350..4441
                     /locus_tag="KQD82_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG4381943.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            5107..6447
                     /locus_tag="KQD82_003504"
     CDS             5107..6447
                     /locus_tag="KQD82_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG4381944.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVRFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2626 a          608 c         1146 g         2262 t
ORIGIN      
        1 ttgattgtgt tacttattaa tagtatttaa gtatttactg tactacttat taactatatt
       61 aagtattgat tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt agaagttagt
      121 tttaataaaa attagtttta gtatttatct agtcatatct atcatgcatg tggatacgtt
      181 ttaaaaattt taaatttatg aaaatattat aatatcacta taaatcaaga gattaattta
      241 atataaaaat ataagaatat tggtttacag ctgattgaga tgaaaaataa tgggtaatta
      301 atatattaca ttaatgttga ttgatgtata actatctgtt ataatgaaaa tatattaaaa
      361 atcatagtgt tgaagaggaa gagtagagta gaaaccattc tcagagaatt tgggtctatg
      421 gctgagagcc catgtaatag gtctatttga aaactacctt ggagctgagc ttattagcag
      481 tatttgtatt tgtactatac gtgaaagtgc tcgggtgacg accttatagt catcaagagg
      541 gttgtatata cttgtatacg ccaacttggg tggaaccgcg aattaatatc gtcccaaaca
      601 tatttggggc gattttttat tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa
      661 taatgattaa agttgcttta aaagatggct caataaaaga atttgaaaat gctatttctg
      721 ttatggatgt agctaaatcc attagtgaag gattagctag aaatgtggta gcagcgtctg
      781 tgaacggaga ggttgtaggt ctggaccaca ttatagatac tgattgtgac ttgaatttat
      841 ttaagtttga agataaagaa ggtaaggaag tttttagaca tacatcagcg catatattag
      901 ctcaagctat aaaaagactt tacccagaag caaagcttgc tataggacca agtatagaaa
      961 atggattcta ctatgatata gatttagacc atagattagt tccagaggac ctagagaaaa
     1021 tagaagctga aatgaaaaaa atagctaaag aagatttaaa aatagaaaga tttgaacttc
     1081 ctagaaatga agctttagag cttatgaaag aacaaggaga agactataag gtagaactta
     1141 tatctgattt acctgaaagt gaaataatat ctttctacaa gcaaggtgat tttactgatt
     1201 tatgtagagg accacattta ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg
     1261 tagcaggtgc atattggcgt ggtgacgaaa ataataagat gcttcaaaga atatatggaa
     1321 cttcttttga gaaaaacaaa gacttagaag agtacttaca tttactagaa gaagctaaaa
     1381 agagagacca cagaaaatta ggtaaagaat taggattatt tatgatacct gaagaaggtc
     1441 caggattccc tatgttttta ccaaaaggta tggaacttaa aaatgaatta ttaaaattct
     1501 ggagagaaat acacagaaaa gctggatata ttgagataga atctccaatc atattaaata
     1561 gaaaattatg ggagacttca ggacactggt atcattacaa agaaaatatg tatacagtta
     1621 aaatagatga tgaggattat gctataaaac ctatgaactg tcctggtgga ttaatttact
     1681 ataactctca actacattca tatagagatt tcccaatgag agttgcagag cttggtagag
     1741 ttcatagaca tgagttatct ggtgcattac aaggattaat gagagttaga gcatttactc
     1801 aagacgattc acatatattc atgttaccag agcaaattaa ggacgaaata aaaggagtag
     1861 ctaacttaat agatggtatc tacaaaactt ttggatttga atataattta gaattatcta
     1921 caagaccaga gaactctatg ggaagcgacg aagagtggga agcagcagaa aatggattaa
     1981 gagaagcttt agaagagtta ggtctaccat atactattaa tgagggtgat ggagcattct
     2041 atggtcctaa gatagacttc catctaaaag attgtttagg aagaacttgg caatgtggta
     2101 ctatccaatt agatatgcaa cttccaagac aatttgataa tacttatatt ggtcaagatg
     2161 gtgaaaagca tagaccagtt atgattcata gggttgcttt tggtagtata gaaagattta
     2221 taggtatatt aatagagcat tatgctggta aattcccagt ttggttatct ccaactcaag
     2281 ttaagatact tcctatatct gataaattta tggattatgc aaatgaagtt aagaaagagt
     2341 tatttgataa gggtataaga gttgaattag atgatagagc tgaaaaaatt ggatttaaga
     2401 taagagaagc tcagcttgaa aaagtgccat acatgttaat agtaggtgaa aaagaagtag
     2461 ctgataataa tgtttctgtt agatcaagag ataaaggtga aataggtagt attaaattag
     2521 atgaatttat agctagtatt tcaaaagaaa ttgaatcaag agaaagtatt attcaagact
     2581 aatatatatt attttagtag ctaagatatt gttatataag ataagtttaa tcaatatgtt
     2641 gatatgttga tgcatagtga ttaaatttat gaaaagatga gtataattag aggtacagtt
     2701 aatcatattt ttatgattaa tgtacctcta gttttttgta tggtagattt attaataaat
     2761 atatcataga agtgttttga acaaggaaat atttatgtgc attctttaaa taaaagttta
     2821 ttttttgagt gataattaag tgcttgttgg taaggttatg gctgtaaaaa tcataacttt
     2881 attttcatat ttgttccatt ttcataaaaa ttattatacc tttaagaaaa tttcttaatc
     2941 ctcttgaaat atttcctttt tagtctaaag ttaaatttct ttcttcaaaa tgaatattta
     3001 aatgaactaa aatttaagta cattttctaa tcttatgagt ctatttttca tagataattg
     3061 ccctataata gaagaatcta tgttggtgct atgtggtgac aataacagca ttgtcagtga
     3121 ttaattaatg ctttataaat gattaattag tgttttatgt tatttttgga cttaccctct
     3181 atacatagat taatttattt aactttagat agagagagaa tgtcaagtac atacacagac
     3241 attagagttt aagaaagtgt ttatggagca agaactttta aaagaggtag tgacttagtt
     3301 taatttcaag aaatgataaa tacaaaagag gaaaaagaga ggtgttgaaa tgatagaaaa
     3361 agtagaagaa aaatctaaga ttattaaaac atcaaaagaa tataagcgaa ctataattga
     3421 agaaaaagat aaagaatgga ccaaaaaatt aaatgatata cttagtagaa gtagtttttc
     3481 taatgatgaa ttagataaaa tcaaggcgtt aattccaaaa gagattttga ctaatgaaaa
     3541 tgttggagaa gttgtgactg aagatggata tgctaagccc gagtatgatg aggtatttaa
     3601 atcattgttc actttaaata atgaatatga tttacttgta aattttataa atgatatcct
     3661 aaaagatgct ccatatgcta acaggaatat caaaccgttc actcaaataa agagaataat
     3721 tagagttgaa actgacccaa caattaatta cattggagag aaaagaccta gacttgatat
     3781 tttggctgaa gatgaagaaa ataatcatat aaatattgag atgcaaagag cttttgagaa
     3841 cgattatttg gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga
     3901 aggtaaagag tataaagaaa ttggaaaaac tattggaata catatactaa atcatgtaaa
     3961 atataatcac atagaagatt atgtaaattg cctgagatta acaatggacg ggcatccaga
     4021 tatttattct agtaaaactg cattatattt tatagagctt ccaaaaattc ataaatctga
     4081 ctatatagta aatagaatta tgctatgggg aaaacttatt agtaatcctt ctagcttaga
     4141 tgtaagagtt atagctaaga atgattctat tattaaaaaa gcacttgata gattaaaaga
     4201 attaggtagc aatgaagaat atttattaaa tctgaaaaga ggtgcttata tcatgaataa
     4261 aagtagaaat tttaaagaag agattgaaag agagacagca attagagttg caaaagaaaa
     4321 agattataaa ataattataa tgttgaaaaa aaatggtttt aaattagatg acatattaaa
     4381 taaatttaat gattttgact taagtgaaga tgagataaaa gaagtatatg aaaataattg
     4441 atgataaaaa gtgaacttat ataagtttgc tgaatcggag tctggaaata tttatgtgta
     4501 ttttttaatg aataaaaatt tattttaaaa acaataattt agaggtacag ttaattatat
     4561 ttttatgata ctgtacttct agttttttct attgtagatt tattatttac taataaatct
     4621 acaatagaag tgcttttgtt gtaagaatca ttttatctaa tgattgtttt ttataatagt
     4681 taaatttcca tttgtagtgt ttaaatataa atctataaca gcatttctat aaatgttata
     4741 aatcactata atgattcaac tcaaagttac tgcttaaaga agcagagatt ttatcaaggt
     4801 atatcatgga aaaaaatatt ttattgtaat atttataaga ttttattatg gaaataatgt
     4861 aaaaacttta taaataatat attttatacc tagttataga taaatttata tagttattat
     4921 taaaaaaagc aataaaaagt ttaattcaat ttagcaaatt tagtgatata attaaaaaag
     4981 aaaagaaata tggacttatt tggaataatt ttgacaatct atttactaca cgtgtagagt
     5041 cgtatataat taaattatac ataaaatata aaaaaataat ttagtaatac gttaggagaa
     5101 agtattatga taaagtcaag cttattttgg aatattttca ttgcgatatc ctttatgata
     5161 gaatggtttc tttgtaagaa aatgctagat tatacaagtg agaaaaggat ttctaacaaa
     5221 aaaataaact tttgtatggt aggtattatt gtttttgctg acataatata tttttttgat
     5281 gtacctccaa atataagagt aattatatct atgataatta ctattgcatt ttataaaatc
     5341 tcatatgatg tgaggtttat aaagtctgta ttaatatcct taatttattg gatgttacta
     5401 atcgccacag atgctttgag tatatcttta acagtttggc ttaattctat aaatgacatg
     5461 agtagtatgg ggacgggaga tatatacaga attgagtcta taatattggg aaagatactt
     5521 ttgattgtgt ctttgctttt atatagagcc ttaaagataa aacttaatat agaaataaat
     5581 aaaaggacat tattttatat agttattcct atagtcgcta atatagcatc tttttttgta
     5641 atttttgaat acatatttaa tccagggtct ggtagcttaa tacataattt ccaattcgtt
     5701 atgatttctg ttgtattgct tatatcaagt atcactttag ttttaattgc taaaaaatta
     5761 cagcaatata atactttagt tgtagaaaac aatatgatta aaaatacttt gaagtatgaa
     5821 aatgagtatt atgaagatat agaaaagaaa tatttaaaaa ctagattact aagtcatgat
     5881 atgaaaaatc acatattgtg tataaatgct atgattaaaa aaggtattga tgttacttct
     5941 tatttaaata atttgcaggt agaaattcag agtaatgact tgttatttaa tactggtaat
     6001 tttatattag atgctatatt aagtgaaaag aaagaggttt gtgataagaa tgatatagaa
     6061 ctaaaaatag gaataaattt tagttgttgt gaagctgttg aattagtgga tatatgtcat
     6121 atattttcaa atattttaga caatgcaatt gaggcatgta ataaaattga tagtggaaat
     6181 agagttattt gtgttaaagg tgatgttatg caaaattttt atttaataag agttgaaaat
     6241 actaagatga ataagattat tactaaaaat ggagatattt tgacagacaa aaaaggttct
     6301 cttcatggac ttggaataaa aagtgtaaaa aactctgtaa ataagtatgg tggtaatgtt
     6361 gtaattgaat atgatgataa aaaatttgta gttaaaattg ctattccact tagaaatgtg
     6421 aagaaagcag ttaatcatgt gatttaaatg tatataaatt tgatgtgtag aaatttaata
     6481 tataagaatt aatgtataaa agtgatgcat agaagtttaa tatataagaa ttaatgtata
     6541 aaagtgatgt atagaagttt aatatatagg aatttaataa gtataaaagt gatgcataga
     6601 aatttaatat ataagaattt aatatgtata aatgtggtgc at
//
LOCUS       DAENCV010000175         6584 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAENCV010000175 DAENCV010000000
VERSION     DAENCV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.178"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(619..1179)
                     /locus_tag="KQD82_003505"
     CDS             complement(619..1179)
                     /locus_tag="KQD82_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG4381945.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="KQD82_003506"
     CDS             complement(1225..2208)
                     /locus_tag="KQD82_003506"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG4381946.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="KQD82_003507"
     CDS             complement(2221..2583)
                     /locus_tag="KQD82_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4381947.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="KQD82_003508"
     CDS             3826..4473
                     /locus_tag="KQD82_003508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381948.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="KQD82_003509"
     CDS             4748..6385
                     /locus_tag="KQD82_003509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381949.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAENCV010000176         6575 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAENCV010000176 DAENCV010000000
VERSION     DAENCV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.179"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            395..1669
                     /locus_tag="KQD82_003510"
     CDS             395..1669
                     /locus_tag="KQD82_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG4381950.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVID
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENIFKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1876..2193)
                     /gene="trxA"
                     /locus_tag="KQD82_003511"
     CDS             complement(1876..2193)
                     /gene="trxA"
                     /locus_tag="KQD82_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG4381951.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2460..3212
                     /locus_tag="KQD82_003512"
     CDS             2460..3212
                     /locus_tag="KQD82_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG4381952.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGEKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3199..4080
                     /locus_tag="KQD82_003513"
     CDS             3199..4080
                     /locus_tag="KQD82_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG4381953.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4306..5661)
                     /locus_tag="KQD82_003514"
     CDS             complement(4306..5661)
                     /locus_tag="KQD82_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG4381954.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2524 a          941 c          827 g         2283 t
ORIGIN      
        1 tttttttcta attatataca atatattttc cattatgttc taatcaattt ctttactatt
       61 tttacaactt tattagtcat attcataatt atttctaatt agtgtttaac attttatgta
      121 aaatttataa aaaaatgtat ataaattaaa ataagtgact atactaaaaa tatgatacgg
      181 ataaacaata agttcaacct atctccccca aaatcaagtg agctacagtt tcccccaact
      241 gtagctcatt ttcgtgtaat ttaataaaca tctcaacaag aattaattaa cgaaacccct
      301 tataaaaaca taacatatat taaacaagct cttgattaaa acttgagctt ttttaataaa
      361 aaacagaata gtttattaga taaggggttt ttttatgcaa ttcatggatg tatctaaata
      421 cattgaaggt gcaaatctta aagttaaaac taaaaatggt gaaactgtta aaagaatata
      481 ttttaacaac tctgccactc cattagtttt aaaaaacgta atagataact taaactgtga
      541 gattccttgg cttacttata ttaatgctcc aggaataatc tcagaaaaaa atactttaaa
      601 atatgaaaat gtacgctcta ctatcctcaa acttattgat ggagacgaag aaaaggattc
      661 tgttatttat gtcaaaaatg ctacagaagg aataaacttc ttagcaaaat tttttaaaga
      721 tgaaaatcca gataaatttg ttattaccac agcaatggag catatggcta attacctgcc
      781 ttttaaagtt aattttccta caaaagttgt tggaattaca gaaaaaggag aacttgattt
      841 atgtcaatta gagaatatat ttaaaaatta tgctggtaat gtatctctcg ttacagttac
      901 aggagctagt aatgtcacag gtataaccac tcctatatat gaaatagcaa gaatagctca
      961 taagtatggt gcaaagatat tggtggatat tgtgcaagta attcagcata aacctttttc
     1021 aatgatgcca catgaatgtg atgagcatat agattttgtt gtttttagtg ctcataaatg
     1081 ttattctcca ctaaatggag gagctctggt aggacctaaa aaattctttg aaaaatttaa
     1141 tcctgtacta tatggctcag gttctaccaa atttgtaaat gataacaaaa tactatatgc
     1201 aaacgtaccc caacgttttg aagcaggata cccagagtat tttggaactc ttgcaatggg
     1261 aaaagcactg aacactttaa ataaaattgg tctaaacaga atttcaaaat acgaaagaga
     1321 attattttta tatgctaaag aagaaatgag taaaatacct aatataataa tatatggaga
     1381 taaatcaaat aatgtaatta tcccaaatat atcttttaat ataagtaata tgtattacaa
     1441 agatgtagca aaatatttag ttgataactt tggaatagaa actggagctg gagtagtagg
     1501 tgcagatatt tatgtacaaa aattattagg tataaatcca aaagaggcat acgtcagatt
     1561 tatgagggaa aatcctgtag gtcttgttag gataagctta ggaatgtaca atacttttga
     1621 tgaggtggat agctttatac atgctattaa aactttggct tttaaataag ctttttctat
     1681 tcttttttat caataaatag agagtagttt tgttttcaca aaaaagcaaa ttatatcaac
     1741 agtcaatttc attagcaatt agacactcat ttgtaactat acaaactatt ttacactata
     1801 caaacaaaaa actcattaca atgagttttt ttgtttatat atgagattta ttttaattac
     1861 ttcaatttac tttaattaca tatatgattc aattttgttt tttaaattct ccttaggcat
     1921 aaaacctatt aatctatcta caggttttcc atccttaaat attagcatag ttggtactgt
     1981 agatacttca aatttctgag ctaattctat actttggtct atgtccacct tcaagaagtt
     2041 tgctttttca accatctcat ttcctaaagc ttcatatatt ggtgatagca tcttacaagg
     2101 tccacaccaa gtcgcaaaga aatctacaac tactaaacca tctttatttt ctacttcatt
     2161 tataaattca ttattattta ttatttttac cataatatac atctccttta ttatcttctt
     2221 gatattaata tactacgccc tatataacag ttcaattagg cacttttttg taaccgccta
     2281 tttttaatag gtttaatatt atgataaata gatttataag attaaatatt tataagacta
     2341 aatatttcta atatatttta tttttttctt aatatagttg acattttttc ccttaaatta
     2401 taaaatcaaa ttaataacta cataggtagt tactaatctt ttatcaaagg agtttctata
     2461 tggataatat aattaatgaa ttacaaaata taggatttac taaatacgaa tctcaaatat
     2521 acatatcatt attaaaggaa agccctttaa caggatatga aataagtaaa ttatcaggtg
     2581 taccacaatc aaaagtctat gaaaatataa caaagctatt aaacaataat ataattatca
     2641 atgttggaac tgaccctatt aagtacatac caataaaccc taatgaatta ttaaaaaata
     2701 agaaaaaaga atttgacaat tctatggata atctaaagaa tagcttacag atattaaata
     2761 aaagtaaaag tgtagaatat gttttaaaca taaaaggaga aaaaaatata ttaaaaaaag
     2821 caatagaaat gataaaaaat gcaaaacaag aaattataat ttcttgccct tatgaagaaa
     2881 ttcttgaatt aaaaaaagaa ctcctaattg cttacaaaaa aaatattaat attgaaatac
     2941 tacttttaga aaacaaaagt ctgaaaggtc ttgataatat tcatattcat ggaggtaata
     3001 aaactacact tgaaaataaa atgctttaca aaggaattat tttgattgta gataatgatg
     3061 aaatattgac aggaaactta tcagaaggca gtgaggccat ttcaatttgg acaaaaaact
     3121 ctaacatttt atatattgga gttcaataca taaaacatga aatttatatt tcagaacaaa
     3181 taaaaggagg ttataaaaat gaacaaaatt aagattaaac aagttgatgc ttttacaact
     3241 ataccttttg gtggaaatcc tgctggtgtc gttactgatg cctctaacat ttctgatgaa
     3301 attaagcaga atgtggctaa agaaatgaat ttatcagaaa ctgcatttgt ttcagagtca
     3361 aatattgcag attttaaagt acaatttttc acaccaggtt tagaagttga tttatgtggt
     3421 catgcaacaa taggtacttt ccatgcactt ttggaagaag ggaagcttaa tactaataag
     3481 agtatatttt atcaagaaac aaaagctggt gtattatctg tagagcttaa gaaaataaat
     3541 aatgaaaata tatttatgat ggaacagaat acacctgaat ttgaaaattt agataattgc
     3601 agaaaagaac ttgcacaaat gataggtgtg catgaagata gacttcttga atatcctatt
     3661 ataaaggtaa atactggact ttggtggctt gtatttggtc taaaatcttt agatgattta
     3721 aaaaatatct ctccagattt aaataaaatc aaaaaattta gtggtgataa taatataatt
     3781 ggtataactc cattttgtat agaaactata gataaaaagt gtgactacca tttaagatct
     3841 atagcaccat atgttggagt tacagaagac cctgtatgtg gaactgggaa cgcttgcgta
     3901 tcatcttata tagtacatca caatatttta gataaaacta gttttattgg tgagcaaggt
     3961 aactttataa acagacctgg aaaggtatat gtaaatataa ctagagaaaa caatgacata
     4021 acttctgtaa aaattggagg aagtgcttat actgttttaa gtggtgaaat tatgtattaa
     4081 tcccatacct aataaaaaca aataaaaata ctagctaact aaagctagta tttttatttg
     4141 ttatttttat ttaataatca tactctaaca aatattataa ttatatatat cataatattg
     4201 tatttagaag taaaatttta tttttattat taattagtta taaatcaatc taatacaaca
     4261 taattacagt agattaacct ataaaacata ataattgctt actaattatt tatcatcctc
     4321 aacaagtcta cttaatgtct tatcagataa gaataataat atcgctgcaa caaatagtat
     4381 tactggaatt atagtatata ctttaatcat atcaaattta agagtaaatg catatatata
     4441 tccatatcct ttaccagcta taaaagtagc aaatgtccat actcccataa gaacacctag
     4501 aagcttctta ggtgcatact tacttacaaa agagttacca agtggtgaga ataacatttc
     4561 accaagactt aaaagaatac caaacatgat tatccatagt atactagctt tagcagtttc
     4621 aggagctcca actcctcttg aaaattctgc accaacaagc attaagaatg caagacctaa
     4681 gaatattaat ccaagacctg tctttttaaa taaactcata tctccttgtg gtctactagc
     4741 aagtttaaac cataatgctc caagaactgg tcctaatact atacatacaa gtgaatttaa
     4801 tgagtcaaac caagcaagag gaacatcaaa tcctccaaca ttcatgttta caaatgctgc
     4861 accataatca taaactgcaa gataagttag ataccagaat acccagaaaa ttacagagaa
     4921 gaatgatata agtataatag cccaaattct cttcttatcc atgcttgtaa gaggagactt
     4981 atctttttct tcaatttttt cagcaactac accttcttta aaaggtctct ttcctgcatt
     5041 cccaaggaat ctccatccat atatgaacca tattgcacct attacacaaa gtacagctgc
     5101 tataaagaaa cattgagaaa atccaagtac accatttttt gcaaatgttt taaggtaaag
     5161 aataccaact gctatagtac caataaaaga acctatatta acaaaagaat actgtactga
     5221 aaatgctgta tcaagttctt cttgactatc aaaaagctgt ccatttaccg cagatacgtt
     5281 acctttaaag aatgcagtac caattgaaac taagattatc attgcattta ccatagaaac
     5341 tgtagttgca atagaaccaa gatagtatcc tactcccatg ataagcatac ctataggtat
     5401 agtatatcta gctcctatcc atctatctga tatatatccc ccaattatag gtgcaaggta
     5461 tgtgaatgca acaaggttgg cttgcattat agcagcttgt cctttatcta ttccaagacc
     5521 accatgaact atagctgttg taaggaatat atatattaag tactttgaag cataatatgc
     5581 tgctctttca aatgagaaag ttgttgagca gatataaaat cccataggaa atttaccctt
     5641 cttttttact gttgagctca taattttaaa tcctttctta attgtatttt tattttttac
     5701 gttttgtcca aattctttct tttatgaaag atataatatt attattttgt aaaacaattt
     5761 gtaattatta ttaagataat tgcgcattta tttgtagagt acatattctc atggaataaa
     5821 attaatacac ttcttaatca taaatttaaa ttatataaat tcactatttt cagatatagt
     5881 tataaattaa tatattctca tctaatacca tgaaaaaacc ttcgccatat gcaattctta
     5941 ttttttacgt gatagtattt tacaaactaa aatccccctt ttgtagcaac ttatgtaatt
     6001 acattttagt gcttttttaa aggagtctcg aataaagcaa catatatttt gttcaaataa
     6061 tgtttttatt tattttttaa atttatatat tacttcaccc aaccaattct caacttaaat
     6121 aactaaacta ctatctattc acagattagg cttatttctt acacattagt ttataataaa
     6181 cactgtgaat ctaaacataa aaactgttat tttaaagtaa ttacccatat attcgctttg
     6241 atactattta ttgaggttat actctttatg tataatatat tatttataaa atacctcaaa
     6301 atatattatt cataaaatac ctcaaactga aaactttttc acgaatattt atttttcacc
     6361 tccccttttt tatttcacca tgtaaatcgt accataaata tgcacttata tgcaagaaaa
     6421 aaatctatta atcgacagta aataattaga aaaattcaaa ttatatttat caagtatatt
     6481 atataaaata tttcctatga aaatataaat taaattaatg ttcagaattg tttttagata
     6541 tgaaatcttg atttttttat tactttactt atttg
//
LOCUS       DAENCV010000177         6497 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.180, whole genome shotgun sequence.
ACCESSION   DAENCV010000177 DAENCV010000000
VERSION     DAENCV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.180"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(84..692)
                     /locus_tag="KQD82_003515"
     CDS             complement(84..692)
                     /locus_tag="KQD82_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG4381955.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(801..3539)
                     /locus_tag="KQD82_003516"
     CDS             complement(801..3539)
                     /locus_tag="KQD82_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG4381956.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3687..4478)
                     /locus_tag="KQD82_003517"
     CDS             complement(3687..4478)
                     /locus_tag="KQD82_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4381957.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4514..5170)
                     /locus_tag="KQD82_003518"
     CDS             complement(4514..5170)
                     /locus_tag="KQD82_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381958.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5163..6128)
                     /locus_tag="KQD82_003519"
     CDS             complement(5163..6128)
                     /locus_tag="KQD82_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG4381959.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6234..6340)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2053 a         1102 c          591 g         2751 t
ORIGIN      
        1 tcaaactaaa aattctataa aaaaataaga tacataccta taaaatacct tcctatagat
       61 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
      121 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atccttacta
      181 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      241 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      301 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      361 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      421 acattttcta tatttgcaga taaattcaag caattataca taccataaat taaataaaca
      481 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      541 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      601 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      661 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      721 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      781 acaataaatt tagacattta ttatttatat atatcaaaaa attctatctc aaacttttca
      841 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
      901 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
      961 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
     1021 ccactgtttt caatttgttt aaatcgatta aatatatcat ttaatttttc tttgtctatg
     1081 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1141 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1201 ctttctatcg attctgggtc aagccctacc ataacagttt cttcatctgt atcaaaaatt
     1261 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1321 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1381 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1441 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1501 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1561 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1621 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1681 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1741 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1801 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1861 aatgctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     1921 ggtataacat ccagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     1981 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     2041 tagtctccaa ctctaacttc ttcaacatgc tcttcacctt ctgagtcaga catcacaaaa
     2101 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2161 agatttaatc ttttaaactc cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2221 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2281 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2341 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2401 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcatcttta
     2461 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2521 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2581 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2641 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2701 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2761 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2821 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     2881 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     2941 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     3001 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3061 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3121 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3181 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3241 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3301 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3361 tcatccgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3421 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3481 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3541 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3601 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3661 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3721 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3781 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3841 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     3901 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     3961 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     4021 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4081 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4141 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4201 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4261 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4321 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4381 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4441 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4501 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4561 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4621 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4681 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4741 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4801 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4861 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     4921 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     4981 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     5041 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5101 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5161 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttact
     5221 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5281 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5341 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5401 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5461 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5521 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5581 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtc
     5641 gcttcatcac aaagaagtac ttgtggattt agtgctaaag ctctagcaat tgcaaccctt
     5701 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5761 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5821 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     5881 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     5941 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     6001 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6061 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6121 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6181 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6241 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6301 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat ttaattctat
     6361 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6421 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6481 taacacttta tatttta
//
LOCUS       DAENCV010000178         6330 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAENCV010000178 DAENCV010000000
VERSION     DAENCV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.181"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            43..681
                     /locus_tag="KQD82_003520"
     CDS             43..681
                     /locus_tag="KQD82_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4381960.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVIGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            819..1481
                     /locus_tag="KQD82_003521"
     CDS             819..1481
                     /locus_tag="KQD82_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4381961.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            1481..2662
                     /locus_tag="KQD82_003522"
     CDS             1481..2662
                     /locus_tag="KQD82_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4381962.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTLWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGAKFIIKFY
                     T"
     gene            complement(2843..5503)
                     /locus_tag="KQD82_003523"
     CDS             complement(2843..5503)
                     /locus_tag="KQD82_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4381963.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWDSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKISASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5500..6183)
                     /locus_tag="KQD82_003524"
     CDS             complement(5500..6183)
                     /locus_tag="KQD82_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4381964.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTYGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2222 a          973 c          871 g         2264 t
ORIGIN      
        1 accatcagta tactttttta tcaatagaaa ggaataattt atatgtcaga attatcaaaa
       61 agagataaag aaaaaataca aagagaaaat gagattattg ataaggcaga aaaattattt
      121 tgtcttaatg gatttgataa tacaactatg aatgaattag caaaagaagt tgaatatact
      181 aaacgcacca tatacaagta cttttcatgt aaagaagatt tattctttgc tgttgtctta
      241 agaggatata aaagactttg ggataatgtt aaaatagaaa gtgcaaaagg caaaacaggg
      301 tttgaaaaaa taaaactttc atactttgct tttcataaat tttactctgt agaaccttct
      361 ctattatgcc ttatgggaat gattggtatt gtaaactctg aaaaatcgga tactgatatg
      421 ctgtttaaag aaaagttttt ttcttttaat aaatttatgt ttgatgaaat acaaggaatg
      481 tttgaaattg gaaaaaatga tagaagtatt aggcatgata ttgaaatacc tactcttatg
      541 tattcatcaa tatttacact tacaggattt tttaatttac tatctgtaat aggaaaatca
      601 tacctgaaca actttaatat agatgaggaa aaatttattg aaacaactct tgctctttta
      661 atagattcaa ttaaagcata aacctaagtt tataaattaa agtttatata cttaaattta
      721 aatgataatt ttttctatct ctaatataaa aaatattaaa cataaaacta ataacttagt
      781 taatggaata actaaattac tgaaaggatt aaaagtaaat ggctaaaata cttcttttag
      841 aagatgatga tacaattaca tttggaataa aaacttccct tgaaaagaaa aatcacactg
      901 tatgttgtta taatacgatt gctgaagcta aaaaaaattt tgataaaact attgatttaa
      961 ttctgcttga tttaaatcta cctgatggta ttggatacga gttttgccaa tatgttaaaa
     1021 gtatagaaga tactccagtg atatttttga cagtccgtga tgatgaaaaa gacattatac
     1081 gtggattaga tatgggtgca gatgactatg tggttaaacc gtttttgctc tcagtacttc
     1141 aatccagaat taatgcagtt cttagaagaa ctcattcaaa tactaacaat actattacat
     1201 gtgaaaatat aagcttgaat aaggttgaag tcaaagcata tttagataat gttgaagtac
     1261 ctttgacagc aggagaatat aaacttcttc taagtcttat ggaaaataaa aatagagcta
     1321 tgactcgtac aaagttacta gaaatgctct gggatgtaga tggtaatttc gtaaatgata
     1381 acactcttac tgttacaata aaacgtctta gagaaaaatt aaaaaatcca ccttgtataa
     1441 aaacattaag aggcatagga tatagaatgg aggacaacta gtgaaaactc caagaaatat
     1501 caacagtaca gttgttttta taatattctg cttaattgta acttttttat tgagtgtaat
     1561 tctaacaact ataacttctt ttaatgaata caatgtactg tctaaggctc ttggtgcatc
     1621 atatgagtac aatcctaagt ctacagaaag tatactaaaa tcactgaaaa attctagtga
     1681 taaggatttt aaaacaggaa atgaaatttt atcaaaacat ggatattcta aaagtacact
     1741 ttggagtaaa aacttttttt attttctagt gatatcgttt ggtataattt caacaatggc
     1801 tacagttgta tatataatca aacatagaac aaataagcaa aaactgagta gaataaacaa
     1861 tcttactcaa tacttagaag aagtgaattt aggaaaagag tctgtacttt tacgatgtga
     1921 agatgaattt tctcaccttg aagatgagat atataaaaca gttggggaac ttcgttgttc
     1981 aaaagaattt gctttaaaag aaagacaaac tctttcagat aatcttgcag atattgctca
     2041 tcaacttaag actccaatta cttctatgtc acttatggca caattacttt cagaaaattg
     2101 tccagatgaa gaaatagatt atattaacag gcttacaaat caaatctcaa gacttgagag
     2161 actagtttca tctttactta cattatcaaa attggatgca agtactttaa tatttgaaag
     2221 caaacttata gatgtacatt ctcttcttac ttatgcaatt gagcctatag aaggttcttt
     2281 aagagaaaaa catcagacat ttactatcga ggcttctcct tcggtagaat ttgaaatcga
     2341 tattaattgg actttagaag ctcttttaaa tattcttaaa aattgtagtg agcatataga
     2401 agatggtgga tatatagttg cttattatag tcaaaatcct ctatatgttg agattatcat
     2461 agaagataat ggtaaggggt ttgctcagga agaacttcca tatattttta atagatttta
     2521 tcgtggtaaa aatgctagta aggacagtat tggtataggg cttgctctat caaagtctat
     2581 tatagaacgt caaaatggta ctctacatgc agaaaattgc cataatggtg gtgcaaaatt
     2641 cataattaaa ttttacacat aaaatgctgg attataggta aaatacctat aatccagcta
     2701 tatctatatt ctatgttatc tattgtttta gtaatatact tctatttaca tttaaggttt
     2761 tattaaacaa acaaattaac tatccctata cttgtaagta gtcttaaatc gaaaccttta
     2821 aataagttca aatagatttt gtttatattg tttcattttt tatagattct actatatttt
     2881 cattcttcaa ctgcttgtat cctgtcatat atgatgctat tacaataaaa agtatcaata
     2941 tagtaaaacc tattattgta gatataggtg caaatggtaa atactctata aaatatattt
     3001 catttatacg tagaaaagca tacactattc ctatttgcac aggaatacta agtagtaatg
     3061 gtgtaattcc taagaataaa ccttccaaaa taagcatctt ttttatgcct tctggagata
     3121 atccaacaga acgcaacata gcaaactctt gtcgtctact tctaatattt cctgacactg
     3181 tagcaagtac atttgataat ccaatcatag ccaataaacc tgtaataaag cctactatta
     3241 aattcatggt ctttattcca ctagcctttt gtttttcact ttctaaaaca tcctcaatac
     3301 tataatctcc tgaaccatag tctttattat aaatggactc taatttttct cttactctag
     3361 gaatgtcctc acgattattt actttaaata ctcctatcag cctatttgaa cgtaacctac
     3421 ttacttcatc ataattttta gatatttcct gcaccataga cattggcact atttgcatta
     3481 atgtatattt tgaactagta tctactattt taggaagtgt gcttgtaata gctcccactt
     3541 tcatgttgta agtataatcc cctttatctt cgtcatatac tttttcattt aatttaatgt
     3601 tatctcctat tgaaagattt aaataatcta tatatacttt atttctacgt gtactacgat
     3661 taatatcttc ctccttatta tatacaattg aggttggatt ttttgtatca taaaataact
     3721 ttgggtctac agaaatttgc ctacaatatt ctgtaaaact cttatcatca agcccaatta
     3781 tatttgaaat tatacgaaac ttgccatcac gttctatagg agagtatttt ttagatgaaa
     3841 ctatactttt aaatcctcca tttttcgcaa gttcttttga tgcatctttt tcagtcagcc
     3901 aagtggaagc agatattttt tgataatata gtgaactttt tacaccttct gtattatcta
     3961 atttttcaat gaaagcagac tctacaggtt cactattttc caaataaact gctatatctc
     4021 tttggtcctt ataattatca cttccataaa cagcttttgc tccttcttga tttgcaatta
     4081 tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa agatattgtt gcagtacgat
     4141 aagatttttt acgagcacgt agtgcgtttg atgcaagttc tccttctatt ccaaatatct
     4201 ttcctaatga atatttatgc gactttttga ttttaacact atctccttgt cgtattgctt
     4261 ctataggaga tatctttgat acttttcgtg ctggtataag tgcagaaatc cagactgtta
     4321 ctattgtaag taaaactgct ggcagaaaag caggaatacc ataagtaaat accacttctg
     4381 gaacatcagt atgataatta actgaattaa cataaactat aagcctatta cacaataacc
     4441 atcctaaaaa taaacctatt ggtaatggta taattgtcag taaaaatcct tcaaacacta
     4501 cagacctttt aatttgtttt ggtgatgcac caacactaga taatatccct aactgagtaa
     4561 gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac cattacaaac acggctactg
     4621 taaaaattgc tattgtaaga tacataatag gagttgaaaa ttttgatata ctactcatag
     4681 atgctttctg ttctggtgaa aagactaaca tctttaaaag ataattacta ttgtatttaa
     4741 gattgtattt tccatattca ttagtcttcc accccaatga tttagcaagt tttggaagtt
     4801 ccttatacgt gtctcttata tttttaaatc gcaagtacac agttatacta tcattaggtt
     4861 ttatacttgt tttatcaaga tatccaagag ctgtatatgc aggtattact gatgaagtag
     4921 aaacatctac ctttcctact attgtcaatg tagtctctga aattttttta aaactttccc
     4981 ctttcgattt tggggctatg ggttcaagag gctttccatt taggattcta tctcctattg
     5041 ggagagtaat tttatcacca atttttacac ttggattatt ctcaaaatat tgttttgata
     5101 atgcaatttc attagcatta tttggtcgac gaccctctat tatagtatcc ttttctggca
     5161 tagaatccca atactctgat gtagcatttc tataaattat ataatctctt ttctcatcat
     5221 taattttgcc tacataccat tgtcctttta ccattacatc atcaactgaa gaaaaatttt
     5281 caattaatgg taagtcactt ccataagtat agtcaaacaa ttcaccatgc cagttacctt
     5341 ctttctcaat tgaaagtctt ataaaatctg tccacatggt atacaacagt ccacacaaag
     5401 aagacatcat ggttgtagta agtaatatag ctaacattat agctatacta ctacgtttat
     5461 tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt catttcttca ccaccttttc
     5521 agagacaatc actccatctt ccatagttac aatacgattt gcctctagtg ctattttctc
     5581 atcatgagta ataagaagta ttgtctgatt aaatcgttta tttgataagt ttaacaaatc
     5641 tacaatttct tctgaatttt ttctatcgag attaccagtt ggctcatctg ctaataaaat
     5701 agctggtcta taaattaaac ttctagctat agataccctt tgttgttgtc cacctgaaag
     5761 ttgacttgga agatggttta atctatcaga taatcccaat atagatacaa tctcagagaa
     5821 tctatcctca tccacctttc ttttatcaag taacatgggt aataatatat tttttctaac
     5881 atcaagagta ggtataagat tataaaattg ataaataaga cctacttttc tacgtctaaa
     5941 aactgcaaga gcttcttcct ttaaacttga tatttctgta tcttcaacat aaatctttcc
     6001 atatgtaggt ctatctactc ccccaagcag atgtagcaat gtagatttgc cagaaccaga
     6061 tgctcctaca atggatacaa aatcaccttt ttgaactgat aaatttacat tattaagggc
     6121 tgtcactctg gtttgatttg aaccatatat ttttgtgaga ttttcacatt ttaatatttc
     6181 catatacaat catctccttt atggttaaat tttagcaacc taaaatgaca ttatagtgac
     6241 atgaatacaa atataaatta ctattaaaaa tattgtttag tttttgcaca ttatttaaaa
     6301 ttataatata aaacaaaaac tatgcaatat
//
LOCUS       DAENCV010000179         6211 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAENCV010000179 DAENCV010000000
VERSION     DAENCV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.182"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..392)
                     /locus_tag="KQD82_003525"
     CDS             complement(<1..392)
                     /locus_tag="KQD82_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381965.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTK"
     gene            complement(866..1750)
                     /locus_tag="KQD82_003526"
     CDS             complement(866..1750)
                     /locus_tag="KQD82_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4381966.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2041..2517)
                     /locus_tag="KQD82_003527"
     CDS             complement(2041..2517)
                     /locus_tag="KQD82_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG4381967.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            2784..3959
                     /locus_tag="KQD82_003528"
     CDS             2784..3959
                     /locus_tag="KQD82_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG4381968.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4040..4843)
                     /gene="proC"
                     /locus_tag="KQD82_003529"
     CDS             complement(4040..4843)
                     /gene="proC"
                     /locus_tag="KQD82_003529"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG4381969.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4883..5467)
                     /locus_tag="KQD82_003530"
     CDS             complement(4883..5467)
                     /locus_tag="KQD82_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381970.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2089 a         1034 c          669 g         2419 t
ORIGIN      
        1 attttagtaa attttacggt aagtaaaaac ctctctcctt caaatcctat agcaaatata
       61 cttacaagtt gctttgagaa aataactcct aatatagcta caactatgca tataccaact
      121 attatattta aaacattgtt taaaaacttt agtgcttgct tttcaccttg tttactactt
      181 atatcttgat acattggaat aaaagtagta actatagcag tacctattgc tgcaaatata
      241 atatttggta tattcatagc tgttaaataa ctctctgtat ataagcctgt accataaatt
      301 gatgataata ctaattctct acccattcca agaatcttag agattatagt aactatcatc
      361 aaataaaaag ttgctttagc taccttgctc attttatcac tccacttcta acatacctct
      421 ttttaatcaa gtaaaattta gctcattaga tataattcta tcaactaatt tgccttgctt
      481 agccctatca aaatataatt aacacctatt tcttaatata tcacaaacta cattattctt
      541 catatttttt tatttctcca ataagtttta catttttcct atataaattg tatagcttca
      601 attatattca aagaaatgga gttgctaccc gataacttta aaagtaaaaa acagatttta
      661 ttatcttttt aaactatatt ctaacatatt ttgtccatat tgagaaattt tactaaacta
      721 ttatgtactt tattaaataa ataaaataac agcacaccta taaaatacaa gtgtgctgtt
      781 cataaacaga ttttataaaa aaataattcc taagtagaga ttaacatgct ttctcatact
      841 ctcatatata atgtgtctaa aaatattaat aaaaatgctt taattgtttt aacttacttg
      901 gatgtcttaa ttttcttatt gctttagctt ctatctgtct tattcgttct ctggtaactc
      961 caaatacctt tccaatctgt tctagtgttt tagggtcatc atcatcaatt ccaaatctca
     1021 ttcttaaaac ttgctgttct tgctctccaa gacctgtaag tagttcttct atcttttctt
     1081 ttaaattatg ctgttcaact tcgtgtatta caacatcttt aaaatcagca tctgatggta
     1141 taaattcaac taaactacta tcctcatctt cttttaatgg agtatccaaa gatacaacat
     1201 ttttatctct tcttaataat tccaacactt tgtcaacttc taagccacat gcatctgcaa
     1261 tctcatctat agttggctct cttaataaca catttaaaag ttcttgtttc tttttcttta
     1321 caaaatttat tctctgatga agatgtatag gtatcctaat agtattttcg caatcgtcga
     1381 tatatcttgt tatactttgt tttatccacc aagttgcata agtgctaaat ttgtatcctt
     1441 ttttataatc gtacttttct acagctttta taagtcctat atttcctgct tgaattaaat
     1501 ccaacatatc aatactatct cttttatatc tctttgctat gctcactacc aacctataat
     1561 ttgaatttat aaatttttct tttgctactg aagatgagtt gattatttcc ttagccagct
     1621 ctacctcttc tcctgctgac aacattttgt attcctctat ttcctttaaa tacattttca
     1681 tcgcatttga tacatttgat gaacccacac aaatataatt ttcataatta ttttcttttt
     1741 tagtatccat aatcaaaccc cttctattca tagaatttgc taaaacaaaa ataaataatt
     1801 taaacctaca taatctacaa ttacataata tcccattctt taaatgaatt gttttgtata
     1861 ttatgtatac aaatttataa aaactatacc tataatatta ttctacttct aacagcaata
     1921 tcctccttat atatataatt tattttatta tatgttgtta tattcttagt tttttgaatg
     1981 atatatgtct aaaaaaagac tgtttactaa acagtctttt tttatatata tgctcaatat
     2041 ttaattttca tgatgatgat tacatccaca tccacaattt tcatcatggt gatgatgttc
     2101 ttctaaatct tttgcctgtt gttccatttc atgaaactct tttaccatta tatcataagt
     2161 tggaagagct gcctcagtat cgtattcttc ccctctccaa tcagcgtaca tcatagcatc
     2221 tccattagtt aaaaccaatc tacctgacat agttatatta tccacttctt ctaaaaattc
     2281 tatttgtttt tgtaaaactt ctccttctaa ctccacatct tcatctaaac attgcactat
     2341 cattacaggt gcataataat cttcttcatc tttaacattt acaacaatat ctaaaattcc
     2401 ttctgcacct tcttcaaatt ctgccaactc aacatttgta tcaatcatat cttgaggtac
     2461 taataaatct tctattaaat attttattac tctatctttt actaattctt ttgacattta
     2521 tttttctcct tccgattcta taacattatc ataatcattt ctatattata gtatactaca
     2581 ttaattgttt tttcaatctc ttacaaaaat tattttttta ttatcttcct tatattccct
     2641 tttttattac aaagttgtaa tcgcattgat ttaaaaaata gtttatattt agtataatag
     2701 aaatgtagca catattttta ttcaatatat taataaaaaa atagattgaa aatgtaatgt
     2761 atgttaaatc taggaggaat attatgaagt ttaaaaaaca aattagttct attgttgtgt
     2821 ctactatgct tgtacttggg agtatgaacc tttcttatgc agacggtaca aatgtagtta
     2881 cacttggagc taacttatca gagtctcaaa agcagcaaat gcttaattac tttggagtaa
     2941 aaaaagacca agtcttagtt ttagatgtaa caaatgcaga agaaagacag tatttacaag
     3001 gtgttgcaac agagggtcaa cttggaaaag ttactatctc ttgctcatac gttgaaccta
     3061 ctaaaaaagg aaatggaatt aatgttaaaa cagcaaactt aacttgggta actagttcaa
     3121 tgattgcttc aacccttaca acagctggtc ttgaagatgc aaatgttatt gctgctgctc
     3181 catatcctgt aagtggtact ggagcattga caggaatcat gaaagcattt gaggatgctt
     3241 caggaaagaa acttgatgaa actaagaaag aaattgcttc tgaagaattg gtagttacag
     3301 gtgacttagg agatgatata gggcaagaaa aagcaacagg tgtaatgaat gatataaaaa
     3361 cagaaattgt aaagaatggt acaaaagata ccaatcaaat agcaaataca ataaataatg
     3421 ttgtaaataa ttacaatatt actattacac cagaacaaaa agaacaaatt aaaggtgtaa
     3481 tgaaaaaaat agctgaccaa aattacgatt acaatagcat gaaaaataca ctagataatg
     3541 tatcagataa tgtaaatgaa caattaaaga aattaggtga aagtgttaag ggttctggta
     3601 tactagatac tattggtgga tggtttagtg gaataggaga ctggttctct ggtttattct
     3661 caggcggaga taaaaaagat ttgggaatct taaataacac taatgatgag tctcttggct
     3721 ctgatgctgt aataaattct acagaaggtg ttcaaataaa tccacaaaat aatactaatt
     3781 ctaacaatga tagtgataca aatagctcaa aaaatgatac ttcaagtaat aatgaaaata
     3841 acgatagttc tcaaacaaat gaaaacaaca caacaaataa ttcagaaaat caatcaaata
     3901 ataatgactc agttggtttc tttgaaaaaa taaaaaattg gctttcaggc ttattttaga
     3961 aattaaacaa ataaaaagaa ttgcctaaaa tggaaatttt ctataaaagg caattctctt
     4021 tatttacaat aaatttttat tactcactca tttttttaga tttttctata catgcacata
     4081 ttgcttttat cacagaagct ctaaaacctt cttcttcaag tactttaact gcttctatag
     4141 tagttccacc aggtgaacat accatatctt ttaattcacc aggatgtttt cctgtctcaa
     4201 gtaccatttt tgcagaaccc ataacagcct gtgatgcaaa cttatatgct tgtggtcttg
     4261 gcattcctgc aattactgct gcatctgcta tagcttctat aaataaaaat acatatgctg
     4321 gtgcagaacc acttgcacca attacagctt ctataagata ctctggtact acctcagtat
     4381 ttccaaaaga attaaatact tctgtaactg cttctaagtc ttctttgttt atatttttat
     4441 ttatagaaag agaagacatg gcttcattta caagagctgg agtatttggc atagttctaa
     4501 ctattttttt atcttctcct ataatagact ctatatcttt tatagatttt cctgctgcta
     4561 ttgtaactat aattttattg tcatctataa agtcttttat ctcttccaaa atatcatcat
     4621 atatatctgg tttaacagct acaattacta tatcagaggt tctagttact tccttcgagt
     4681 cttttgtagt atttattcca aaatttgcat ctattctatc gagagttcct tgactatatg
     4741 ctgatgctat aaccatatta ggttcaacta attttgaatt tactattccc cctatcatag
     4801 cgcttgccat atttcctgca cctatgaatc caattttttt cactttagta cctccttatt
     4861 gactaatagt aatcgttaaa atttaagctt ctttaactgc atcagacttt tcaaatggat
     4921 ttggtatact tactaccaaa attttcacta ccttattagt atagttgacc caattatgat
     4981 tgttcttttc actattatag tgtattgtgt ctcctggata catttctatt tgttcatctc
     5041 ccaaaaacac tgtaagagta ccctctaata catacacaaa ctcctcacct tcatgtacgt
     5101 aacaacaaat ttcctcacta ctcttacttg gaagaatttc aattaatctt ggtagcattg
     5161 ttttttcctt taaactagat gataaatgat aatgtataaa tgttgagttt tctacatcaa
     5221 atacttcttt ttcataactt cttaatacag ctcttttatg ctcttttggc ttcataaaaa
     5281 agtaagttaa ttctacatct agtacacttg ctatttttcc taaggaatct gttgccacac
     5341 ttgttaatcc tctttcaagt tgtgataaaa aaccaattga caagtttgtt ttttcactca
     5401 tatccttcaa cgtcatttgc ttttgagttc tgagttgctt tatttttgct cctatgtcct
     5461 tattcaaaac catcacccct aaataatttt aattttttca taatataaat acaaaaatat
     5521 aaatataggt actgaaccta tctttatttc atttaaaaaa atttttataa gtattactat
     5581 aatttatcaa tctttttttg tctacatttt tacgattatt tacgatttat ttttcacata
     5641 tgtatattta atcttgccaa ctaaatatac attttttata agcatttact ctttatataa
     5701 taaaacttaa gttgttcatg tagtcttctt ctggtttaat tattaaaatt tcaaattata
     5761 ttattatttc tacacattat tacatccttt agtacaatta taacataatt ttacaattta
     5821 atgatatcta ttcaatgtct ttttttactc tacaacatat tttattttag agtttaatac
     5881 tatatatatt ttttatatac agccttgtca ttttttagat tgtcaagagt tattgaagta
     5941 ttttatgtag atttagattt tatttcctaa tttctcattt aacaagaatt gcttatgata
     6001 aaaaataata tcatctcaat aagattcttg taaatatatc tatattctaa atataatttc
     6061 tttttttgtc aaaatttctt cattctttaa taattaattt attacttata aaataaacta
     6121 attatctata caaattttat aatttttaat aaaatacaca aaaaaattaa tttttatagc
     6181 tcatttcgcc aaaatatatc tttctttata t
//
LOCUS       DAENCV010000180         6110 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAENCV010000180 DAENCV010000000
VERSION     DAENCV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.183"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(196..2583)
                     /gene="cadA"
                     /locus_tag="KQD82_003531"
     CDS             complement(196..2583)
                     /gene="cadA"
                     /locus_tag="KQD82_003531"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG4381971.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKISIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEADDVINKIKEIINSTEPGLD
                     IQVESTNKVKGRTTEKPGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGISMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2606..2971)
                     /locus_tag="KQD82_003532"
     CDS             complement(2606..2971)
                     /locus_tag="KQD82_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381972.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3171..4004
                     /locus_tag="KQD82_003533"
     CDS             3171..4004
                     /locus_tag="KQD82_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381973.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNMNSNMGIPN
                     NMSNNMNSNMGMPNNMSGNMNNMYGNTSMPNNMNSNMGYNMNNTMSIPNFSCNQNMPP
                     NVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQTTNIEEFDEEE
                     M"
     gene            complement(4152..5240)
                     /locus_tag="KQD82_003534"
     CDS             complement(4152..5240)
                     /locus_tag="KQD82_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381974.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFARVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5365..6093
                     /locus_tag="KQD82_003535"
     CDS             5365..6093
                     /locus_tag="KQD82_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG4381975.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2121 a         1032 c          661 g         2296 t
ORIGIN      
        1 acaatataaa agctctacac taaataaata cattaaataa acataatgtg taatgaatat
       61 agatttatat atacaaaaac gctccaataa aaaggtacca ataaagttaa aagaaactta
      121 ttgatacctt ttattattat atacttcaat atataattat aagttttttg aatagtatgg
      181 atttacattt atcccttatc tcatagctct catagcattt actactgcta taagtgccac
      241 acctacatca gcaaatatag cttcccacat agtagctact cctccagcac ctagtatcat
      301 aactataatt tttactccta gagcaaatat tatgttttgc cacactatct tattagtctt
      361 gtttgctatt tcaattgctt tagatatctt gctaggctca tctgtcataa gcactacatc
      421 tgcagcttca atagcagcat ctgaacctaa gcctcccata gctataccaa catcagcacg
      481 agctaatact ggagcatcat ttattccatc acctacaaat gctattttct ctttttcagt
      541 tcttccttca tataaatctt ctaatctatc taccttttca ttaggaagta aatttgagta
      601 aactgtatcc aattctaatt cttgagcaat attttttgct actttctcat tatctccagt
      661 taacattaca acttctttta cccctattgc ttttaagctt cttatggcat ttttactatc
      721 ttcttttact tcatcagata taactatata acctctatat actttatcaa cagcaatata
      781 gactactgta cctacttctt tagctgatga ataagatata ttttcttttt tcataagttt
      841 ctcattacct gctaatataa aattgccatt atgctttact ctaattccat aagctgctat
      901 ttcctcataa ctgtctattg tatctaaatc aattttttta ttatagtaac ttaatataga
      961 ctttgctatt ggatgatttg aatttacctc cgcaatagct gcatattcta ataattcttc
     1021 ttctgatata ccttcaggat ttaacttagt tacattaaac acacctttag taagtgttcc
     1081 tgttttatca aatactactg tatctacact tcttaaagct tctaaatagt tacttccttt
     1141 aataaggata ccatttttag atgcaaaccc aattccacta aagaaactaa gtggtattga
     1201 taaaactaat gcacaagggc aagaaacgac taagaatatc aatcctctat ataaccaatc
     1261 actaaatacc tcacctgaga ttacaagtgg tggcacaaat gctattaata aagcagctat
     1321 tactactatc ggagtatagt atcttgaaaa cttagatata aagttttctg tctttgactt
     1381 tttaatgcta gaattttcaa caaggtcaag aatcttagaa actgttgatt ctccaaaact
     1441 ctttgtaact tcaatactca ataaagcatt tttatttata acacctgata aaatttcgtc
     1501 tcctttttca acttctctca gtactgactc tcctgtaagt gcagaagtat ctagcataga
     1561 aattccatcc actactacac catctaatgg tactttctca ccaggtttta ctactattat
     1621 atcgcctaca tttacttctt ctggtgacac caccttaact tcagaattaa cttttaaatt
     1681 tgcatagtct ggtcttattt gcataagtga agttatagac tttcttgatt tcccaactgc
     1741 aacaccttgt aaatactctc ctactttata gaaaagcata acccctactg cttctgataa
     1801 ctcccctatt gctaatgctc ctactgttgc tagagccatc aaaaagttct catcaaatac
     1861 tcttcctttt gatatatttc ttattgctct taaaagcaca tcgccaccta ctattacata
     1921 ggcaactata aatactatat tgctaaactg actttcataa cctgtagctg tttgatatat
     1981 accaaatata tatacaagtg ctccaattat aagtggaatt aattctttct tatttgtatc
     2041 atttacagct cctggttttt cagtagttct cccttttact ttgttagtag actctacttg
     2101 tatatcaagc cctggctctg ttgaatttat tatttctttt attttattta taacatcatc
     2161 cgcttcaaag ttattactta tatttactgt aagtttttta tttacaaagt ttaaattaga
     2221 agattcaact tcctttaatt tagaaacttt gttattaatt tcctctgcac aatgtgcaca
     2281 gtttaatcct cctaatataa gctctttttt aattgatatt ttactagatg cattttcttt
     2341 agaacttatt tgtatatcaa gacctggttc tgttgaatct attatatcta ttatcttttc
     2401 tatagtagta tcctcattaa agctttcctt tatatttact gtaagttttt tatttataaa
     2461 atttaaatta gaagatttaa cttcttgtag tttagaaact ttattattaa tttcttctgc
     2521 acagtgagca caatttaatc ctcctaggat aaattctttt ttaattaaat tatttgcttc
     2581 catatatatt cactcctctc atttatcatt tataagtctc tctaatatga tttaatccac
     2641 aatcaaatat ctgacttata tgactatcat ctaatgagta atacactgtt tttccttctc
     2701 ttctaaactt tactaatcta gcctgcttta atactctaag ttgatgagat attgcagagt
     2761 gagtcatatt taataaactc gctatatcac atacacacat ttcatttgca aataatgcat
     2821 ataatatctt tactctagta gtatccccaa atactttaaa taattctgct aaatcatata
     2881 acatctcttc atctggcata gttgatttag cctctgttac tatttcttca tgtactatat
     2941 tacaattaca atcatttatc tcttctctca tttttaatcc tccaattatt ttaatatatg
     3001 aataactgtt catatgttca ttatatgcta tcatttctaa atttgcaaca ataattttta
     3061 ttaatttaat ataattagaa tgcacatatt ataaactttt ttaatatata tattattaga
     3121 ataagttttt gtaaactttt ttatagtttt ctaggaggtg caagtttaat atgagtaaca
     3181 agaaaaaaaa ggatttagat actagttaca tgccttactt agacccagcg aactatgcag
     3241 attatattac taatgaagaa attcctcaag atgaattgag aaatgaccct caaatttcta
     3301 atggttatac aaaaatacct aataaccaga acataaataa cctatatcct aataatgtaa
     3361 atcattatcc taaaagtagt aattctccaa acaacttgcc taacacaaat aatacatccg
     3421 gaaatatgaa ttacaatacg tctgctacaa ataatatgcc taataacatg aataataata
     3481 cgggtatgcc taataatatg tctaacaata tgaatagtaa tatgggtatg cctaacaata
     3541 tgcctaataa catgaatagt aacatgggta tgcctaacaa tatgcctaat aatatgaata
     3601 gtaacatggg tatgcctaat aacatgaata gtaatatggg tatacctaac aatatgtcta
     3661 ataatatgaa tagtaatatg ggtatgccta acaatatgtc tggtaatatg aataatatgt
     3721 atggtaatac aagtatgcct aataacatga acagtaatat ggggtataat atgaacaata
     3781 ctatgagtat tcctaacttc tcatgtaatc aaaatatgcc tcctaatgta cttatgatga
     3841 taccaggagt tatatgtcat aatacaatgc aaggtatgcc tgtagttatg cctagtacta
     3901 tgcctcctaa tatatatcct actccttatg gttctagtaa tatgtctata caaggtatac
     3961 cacagactac taatattgaa gaatttgatg aggaagaaat gtaatatata tttttttaag
     4021 taagttgtat aaaacattaa taattgacca taataataaa tgtattcctc ataatattcc
     4081 cccaatatta tagaaataaa tttaagcgag attaaattca gttaatctcg cttaaataat
     4141 gtctaaattt attattccaa aaaatcatca aaatctaatg aacctgattc tgcaaagtca
     4201 tataatgcat tttgttcaaa gaaaaatttc tcttcaaatt taatatagtc actataatta
     4261 gattgactta tttcattgat ataattgcaa tcattttcgt tataagtacc aataaaaaac
     4321 ttatttccca ttaagtaata tatagctttc atatcattat ttattatata tacattttct
     4381 tttacatgat cattattaaa attaaatcta gtaaaaaata taccttcatt catattactt
     4441 tttaacaaaa atggattttc tctataaaat actcttgcaa attcttcagg tgaatttaca
     4501 ctgtatacac ttacaaattc acttttagaa atctcatcaa atacctcaaa atcatacata
     4561 ggttctttat ctgtcactaa tagagagtta attctaaaac cagtttcata acacttactt
     4621 atgcaaaaag caattttaca tatatagtat ccatcattat cttcatataa agcttcactt
     4681 atatatctgc cttgaccttt atcacttacc acatttttaa gtaatgttcc atttatatta
     4741 gtaatatgca tatttgcaat ctcgtcactt ccagaaaagt actttaaact ttctctatcc
     4801 catgctataa atctcatagt catataattt ataaattcaa cttcttcatc aactctttta
     4861 catattttat tgtataattt ttctatagtt aaatctgttt tatagttttt tataatatca
     4921 atatactcac atttatctcc tggcaatagc ttatcataat aaagattctt attaccaaaa
     4981 taagatacta aaaataaaga ttcatccttc gttaattcaa ctctatctga accaaatcct
     5041 cccatcaatc tttcttcttc catataagct tcttcttgtg ttgggttatt taaactcaca
     5101 taatctgcta atcctaatcc ctctgcatct aataaaaaat attgacaaaa gtgattgttt
     5161 tcatcatcta tccaatttat tataagacct aaacttccca taagtctaga atttgtagcc
     5221 tttgcaaaat taaaattcat atagtcacat cctttaaact ctaaaatact tgttttttaa
     5281 tatataaatt attatacata tattttaaca acttagatac aatttataat ataatataag
     5341 gaaatgatta attgaggtgg tcttatgaaa aatatagcag ctttttttga tatagatggt
     5401 actctttata gagactctct tatggttgaa cattttaaga aattaattaa atacgatata
     5461 attgaccaaa aaaattggta taaacatgct agagacactt ttatggattg ggataaaaga
     5521 caaggtaatt atgatgatta tctattagaa atatgtgacc tttatgttga ttcattaaaa
     5581 ggattagata aaacctgtat caactttaca agtgaccaag taattaaatt aaaagcagat
     5641 agagtctata aatacacacg ttcaaggatt gagtggcatc taaatcaagg acatatagtt
     5701 atatttatat ctggtagccc tggatttctt gttgaaaaaa tggcaaaaaa atataatgta
     5761 accgattttc taggaagtga ttatgtcttt gaaaataata tatttacagg cacagttatt
     5821 ccaatgtggg attctataag taagaataat gctataaacg attttgttgt aaagtatgat
     5881 ttagatttat caaactcgta tgcctatgga gatactaatg gtgatataaa tatgctaaaa
     5941 agagttggaa atccaatagc aataaatcca acaaaggaac ttttatctca aatatcactt
     6001 gatgaggaaa tttctaaaaa tgttgagata atagtagaaa gaaaagatat tatttattct
     6061 ctatctacag atgtaaatat agtggatatt taatttatac tatctatttt
//
LOCUS       DAENCV010000181         6105 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.184, whole genome shotgun sequence.
ACCESSION   DAENCV010000181 DAENCV010000000
VERSION     DAENCV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.184"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            198..1010
                     /locus_tag="KQD82_003536"
     CDS             198..1010
                     /locus_tag="KQD82_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG4381976.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1099..1290)
                     /locus_tag="KQD82_003537"
     CDS             complement(1099..1290)
                     /locus_tag="KQD82_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381977.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1593..1799
                     /locus_tag="KQD82_003538"
     CDS             1593..1799
                     /locus_tag="KQD82_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4381978.1"
                     /translation="MFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSTLDVAVLIGFNS
                     QNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2108..3727
                     /locus_tag="KQD82_003539"
     CDS             2108..3727
                     /locus_tag="KQD82_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG4381979.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            4101..4295
                     /locus_tag="KQD82_003540"
     CDS             4101..4295
                     /locus_tag="KQD82_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4381980.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(4854..5177)
                     /locus_tag="KQD82_003541"
     CDS             complement(4854..5177)
                     /locus_tag="KQD82_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381981.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(5222..5476)
                     /locus_tag="KQD82_003542"
     CDS             complement(5222..5476)
                     /locus_tag="KQD82_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381982.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(5740..6021)
                     /locus_tag="KQD82_003543"
     CDS             complement(5740..6021)
                     /locus_tag="KQD82_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381983.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDIVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         2249 a          764 c          742 g         2350 t
ORIGIN      
        1 taactaaatt gaaataatca ctttttcaag aatgttataa tttttttatt gtattctttt
       61 tgctatagtc tatgaataaa tcaatagaaa atcttctaag ttgcttttaa ataatgactt
      121 tatggttcaa ttgtactaaa atagtatatg taggttacaa ataatctaat aaatattaaa
      181 gtttggaggt aatagatatg gcagattgta attcatgtcc atcaaaggga aattgtaata
      241 gtcaatcaaa ttgttctatt gaaaataacc ctaacaataa atttggaaaa ataattggtg
      301 ttatgagtgg gaagggtgga gttggaaaat caactgtaac tgctttactt gctaataagc
      361 taaacaaaat gggatataag gttggtatat tagactcaga tataactgga ccaagtattc
      421 ctagacttat gggtgtaaaa aatgtaaaag cttattctga tggctcttat atttaccctg
      481 tagaaaactc aaataacata aaagtaatgt ctataaacct tatgatagat gatgaaaatg
      541 aacctgttgt ttggagagga cctttacttg gaggggttgt aaagcaattt tatactgatg
      601 tactttggga agaattagac tatttattaa tagatatgcc tcctggtact ggagatgttg
      661 ccttaacagt tatgcaatct attcctataa gtggaatagt tatggtttca gtacctcaag
      721 atttggtatc tatgattgta tcaaaagctg ttaatatggc taaaaaaatg aacataaatg
      781 tccttggagt tattgaaaat atgagttata tccaatgtcc tgattgttct aaaaaaatca
      841 agttatttga aggagaatct actgaaaagt ttttagatga tttagactta gaacttttag
      901 gagaacttcc tatgactaaa gaaattatag atattactca taatggagtt acagaaatta
      961 gtgatgattt agattcaata ttgactaatg ttgttgaaaa aattaaataa ataaaaattt
     1021 tataatttta tatttatcaa aaaaagaggc ttgtatttat tttataatca tacaaataaa
     1081 tataaacctc ttttatgttt atctatgttt taaatacaca aaatattctt ctacttcatc
     1141 tccatttttt acttttacta aatattcatt ataatgagca ttatatggct gtgtcatttt
     1201 gcattctatt acattttcta tgtcttctaa ttctaaaagt tctacatcct ttaaaaattt
     1261 tctttctatg agttccatta atctttccat aatatatcct cacttcccct ataattaaga
     1321 taaactatat atcctcttaa tacagagttt atcttaatta tattatatcc aatatattac
     1381 agtttctaac atcattcata aattatttta acaaatagag attgattttt taataaatat
     1441 gccttgataa aatgaaacat agaccaactc ttagttttat gtaaagaaaa cttaaagaaa
     1501 actatagatt atattcacaa aaattactca gaagatataa cagtaagtaa tatatgtaat
     1561 gaattacaac ttaataaaag ttgttttttt ctttgtttaa gaaaaaaagt ggatatactt
     1621 ttactaattt tttaagtaaa gttagagttg aaaaaagcaa aaagtatata ttaaaaaaag
     1681 acttgtctac tttagacgtt gctgtactaa ttggattcaa tagtcaaaat tactatagta
     1741 taatttttaa aaagtttaat aactttacac caattgaata taaaaatatt tataactaaa
     1801 aaatatcatt ctaaaaagat gttatttttt tacatatttt ttacaattat agttaattct
     1861 ttgttaaatt gattgtacat aaaatactaa aaatctaagt tcaagtattt aaattgctat
     1921 atatcataaa ttttctaaaa ttaacaacaa cttaaaaatt taacattctc ttaacgtata
     1981 cttaacaaaa cattaatatt tttattgaca aaatttgtta ttctatatta tggaaacaat
     2041 aaattttagt taataaagtt tataattcag ataaaaaaat tattaatttc ttaagggagg
     2101 aaatatcgtg aatatagtaa ttagccttat cggtggactt ggactttttc tttacggaat
     2161 gagtttaatg ggagaaggtt tacaaaaatc agcaggagac aaattaaaaa aaattattga
     2221 actcttaaca agtaatgttg ttatgggtgt actggtgggt acagtcgtta caggtattat
     2281 acaaagttca agtgctacta ctgttatggt tgtaggattt gtaaatgcag gtataatgaa
     2341 tctaagtcaa gctattggtg taataatggg ggcaaatata ggtactacag ttactgctca
     2401 attagtttca tttaatcttg aaggaattgc accaattgct ttaggtatag gtattttatt
     2461 ctatttattt acatcaaatc aaaaaacaaa gcatttatct gagatactta taggtttcgg
     2521 tatattattt acaggaatgg agtttatgaa agatgcagta gctccacttg ctgaatacaa
     2581 agcattcact gatgcacttt tatattttag taaaaatcct gtattaggta tattggcagg
     2641 atttgccata actggtattg ttcaaagttc aagtgcatct atgggtatgt taatagcact
     2701 agcttctcaa ggaatacttc ctctatcatc tgctttacct attctttatg gagataatat
     2761 aggtacttgt gttacttcac ttttatcaag tgttggtgca agtagaaatg ctcgacgtgc
     2821 agctgtaatg catttaagtt ttaatgtaat tggtacaata atatttatgc ttgttttaaa
     2881 taaacctata tcagccatag ttactcattt tgacccaact gatacagcta gacaaatagc
     2941 aaatgcacat actttattta atcttacaaa tgttataata ttattaccat tttctaaata
     3001 catagttaaa ttagcaaata gacttatacc tattaaagaa actgaatcag aaatagtaaa
     3061 taatactaag tacttagatg aaaggatgtt tagcactcca tctatagcac ttggtaatac
     3121 tgtacaagaa gttgtaagaa tgggtcataa agctacaaac tctttggaac attcaattgc
     3181 aggattttta aataagagca atgaagatat taataaagct tttgagtcag aaaaagttgt
     3241 caataagtta cagaaagata tattgaacta tttgttaaaa ctttctaaag aacctcttag
     3301 agatgatgaa cgctttagaa cagatttgct ttttaatact gtaaatgaca ttgaaagagt
     3361 ctctgaccat gcagaaaata tagctgaatt ggccatgagt gttaaggaaa tgaacattag
     3421 tttttctgat agtgcgataa gagaaatata cgaaatatac aataagacaa ttactaattt
     3481 taaggatgcc ttaatagttt tagatgttaa agattttgag ttagcaaata aagttttaga
     3541 agttgaaaat gaagtaaatt atttagaaaa aacttttaga aattcacata tgattagact
     3601 aaataatgga agttgtacaa ttgatgctgg tgtactttat cttgacttac ttactaatct
     3661 agaaagaatt tctgaccact caacaaatat agtaaaacaa gtactaaaac ttaaacaaaa
     3721 aatatagtga ttatttttat atataatctt aatcaatgag aagtaagaag ctatagataa
     3781 tgcttatatc atttatggct tcttattttt tatgtaatac tcaaattttt atagtattct
     3841 cttattatta aacagtttct gcctagaata ttccatatat atatgacttc tttcataaaa
     3901 cttgaactgc ccataaataa agtcacaagt atacataact attttttaat caattataac
     3961 caatatttgt tttatttatc ttcattttaa ttcttcattc aataatgtaa taagattatt
     4021 ttttatttgg atactattat atcatctaaa tatgttatta tatatttgga tattttcata
     4081 ttaataggag ggattctaca atgataaatt atatggtaga aattatatat agcgaaaaaa
     4141 taagcgaagt agaaaacatg ttgaaaattg aaaatataca atatgaattt acttctcgtg
     4201 atggctttat attttttggt ttttatgata tgacagacgc atatagattt gagactttat
     4261 gttcagatga aaaaattcga tttaaaagaa tataatagat aaaaaagtac atatttctaa
     4321 actatttgtt tttcaaattt aggttgtgtt ttaatgtagt gttgatatta taaacaattt
     4381 taataataaa aaagtaggga cattatatta atatgctcct actttttact catacttatt
     4441 aatattattt atcaaaatta ccctacaaac tggaatttat cattctttca taattttaaa
     4501 ctatcatatt taaaatatat aaaattctac atgatttttg tataaacata atatcaacca
     4561 ttatccaatt taaaaatatt ttttagtaat ttccatttca ttttctctta acatattaat
     4621 taaactttta tatgtaaatg gtgtatttac atattattta atatccaaca attatataaa
     4681 gaaatgtgct ctttgcctta taaagaatta taaagccaat tcgttttatt atattatttt
     4741 tctttatacc ttaaaaactt taccttcaaa ttcttattta tctatatatt ataaaaatgt
     4801 tcttaacctc ttttacgaga ttttcttgac atattatcag ttagataaaa taattaaata
     4861 atataatcag caagtatgtt ttttactctt ttaagtaaat ttccacctgc accccaatca
     4921 atttttaaaa ttccaattgc actgcctgtt gaagtagctt taataactgt ttttcctgtg
     4981 gtattttcat aaactactat taatcctgca tcactattat ttcttgcata atacttctca
     5041 aaagttgtaa ttagtataaa tttatcatct cctaaattat aagaatcact aaaaactaaa
     5101 tctgcatcaa gattttcttt tatcattttt tgtgcctcta agggtgtaat ttttacagta
     5161 aatatttctg ttgccatttt ttatctcctc ataagaattt attttagaca aaacataatc
     5221 tctagtattt tctgatatat ttatcaaaca atatccctat tcctcctaac agagctaata
     5281 tagaaccaaa atttctatat tgagaaatag attggtctag ataaatatta tattggtcag
     5341 tatccatgat tccaccattt tgttgaataa tatgctctgc actaaattca cctaaaccta
     5401 tacaacagaa taatactact actccaatta caaaagcaaa tatacatatt gttaatttaa
     5461 aatccttatt ttccatagtt atccctctca taagctaaat ttatttgatt atttttacta
     5521 gattttatat tactcttatt atacaataca acatatattt acatctattt ccaaaattgt
     5581 aacctttata gttttatata tttaaatttt ttaaacaaaa aatacacatc tataaattat
     5641 aaatgtgtat tctgtaccat tactaataat atattatact tttttacaaa tttctaatta
     5701 tttataacta ttcaaaaata aaattcatat aaaataattc tattgtacaa ttgaaacttt
     5761 aataatgtct ccattttttc tctccaagtg aactacttct ccagttaaat catagtcaaa
     5821 tgctttaact aatatttcag taactttttt accatctact atatcatctt ttccattaaa
     5881 taaatccaaa gcattattct caatcattct ctttgcaaaa tttacaggta actttatatg
     5941 tattttatcg cctgtatgtg ataagactct tattcttaaa aacttttcta aattgtttct
     6001 tggtgtttgt tttgatgcca tgctattaaa tcactctcct ttatttttta tttaataata
     6061 aattttattc ttaaaaatat ctttatatgt atttattatg aaagt
//
LOCUS       DAENCV010000182         6071 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAENCV010000182 DAENCV010000000
VERSION     DAENCV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.185"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..330
                     /locus_tag="KQD82_003544"
     CDS             <1..330
                     /locus_tag="KQD82_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4381984.1"
                     /translation="DIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGR
                     QIVKVGKFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLR
                     LITIQNK"
     gene            762..1016
                     /locus_tag="KQD82_003545"
     CDS             762..1016
                     /locus_tag="KQD82_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381985.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1052..1375
                     /locus_tag="KQD82_003546"
     CDS             1052..1375
                     /locus_tag="KQD82_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381986.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1649..1801
                     /locus_tag="KQD82_003547"
     CDS             1649..1801
                     /locus_tag="KQD82_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381987.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1995..2879
                     /locus_tag="KQD82_003548"
     CDS             1995..2879
                     /locus_tag="KQD82_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG4381988.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAIT"
     repeat_region   3185..3670
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3185..3212
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            3862..4014
                     /locus_tag="KQD82_003549"
     CDS             3862..4014
                     /locus_tag="KQD82_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381989.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4134..4948
                     /locus_tag="KQD82_003550"
                     /pseudo
     CDS             4134..4948
                     /locus_tag="KQD82_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5000..5161
                     /locus_tag="KQD82_003551"
     CDS             5000..5161
                     /locus_tag="KQD82_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381990.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5489..5953)
                     /locus_tag="KQD82_003552"
     CDS             complement(5489..5953)
                     /locus_tag="KQD82_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4381991.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVFEDMKNKILRERKYKKRR"
BASE COUNT         2480 a          578 c         1063 g         1950 t
ORIGIN      
        1 gatattattt gcatagaaga tttacaagta aagaatatga ttaggaatcg taaactatct
       61 cgctcaattt ctgatgtgtc atggtcagag tttattcgtc aattagaata caaggctaat
      121 tggcatggta gacaaattgt aaaggtgggt aaattctttg caagttcaca aatatgcaat
      181 aaatgtgggt ataaaaacga ggaagtgaag gacttaaata taagagaatg gatttatcct
      241 agttgtaatg aaactcacga tagagatata aatgcaagta taaatatatt aaaagaagga
      301 ttaagactaa taacaattca aaataaataa cgtatgtaag aaccgtagga actacgggga
      361 tagcctgtgg agaattagta agacatatgt tagtatgcaa aattctgtga agcaggaaac
      421 ctgtgacttt agtcatggga ggttcaggct agtattactc attattgtat tcaaaagcta
      481 aagtggaaac ctagtgaata tatgaattta gaagttaatt agagagcatt agcagccgac
      541 tcaatactta ttaaaattga agatgaagaa gaaacaatga aagaagctga aagagagaaa
      601 aagagaggta gaagaaggta gaatttaaag ttaaaatagt tacattgcta atatatgaaa
      661 atgttacaaa tttggaaata gatggatata tatgttgtat agtataatgt agataatata
      721 tcaccaagta aaaaacaaca aataaatcta aattatgagg ggtggaaaat aaagatatta
      781 aacttacaat atgtatattt gcttttgtaa ttggaataat agtgttattt tgctgtatag
      841 gtctaggtca atttaatatg gattgtatta taaaacaaaa tggtgggtca atagctaccg
      901 aacaatacaa catatattta gaacaatcta tttcacaata tagaaatttt ggttctatac
      961 tggttttact aggaggaata ggaatactgt ttgataaaag tgtcagaaaa tactaaaaaa
     1021 gtatattttt atttaggatg tgatgaatga tatggcaaga gatgtattta gtgtaaagat
     1081 gacaccacta gaagcacaga aaatggtaga aaaaaatttg gaagcagatt tagtttctgt
     1141 tgatatttat aacctagaaa ataataagtc tataatagtt acaatctttg aaaaatatta
     1201 tgcaagaagc aatagtgatg caggtttggt agtgatttgt gaaaatacta caggaaaaac
     1261 acttgttaaa attacctcaa ctggtagtgc aattggacta tttcagattg attggggaac
     1321 aggtaataat ttgattaaaa gagtaaaaaa aatacttatt gattatatta tttaattatt
     1381 ttatgtagat attcttaggt tggtatggaa tttgtactaa cctattaagt aatcaataaa
     1441 actaaataaa catagaaagc acttacaaat atgtaggtgc tttttattga aaaaatagaa
     1501 aataaaataa aaagtaagat ataggaaaaa tatgtaagaa ttatatgtta taataattgt
     1561 agcaagaaga tgtaatctac aatttataga gtggagttca tgtattaaaa aattatcctc
     1621 ccaacgataa gaagggaggt gaatatacat ggataatttt ttatttaatg tgttagctag
     1681 tgtaacagct agttacatag tttatttgat tagcaagtta atcaaaaaag taaaaagcca
     1741 ctctaccgca aagagtgact taaagattga tataaatttt aaatatcatc gtaaaaaata
     1801 actttcagtt atagaacttc actctaccgc aaatagattg tagttcttct tgtttttatt
     1861 ataccacaaa ttagaaaaaa tattgcatat aatatttttt atagtcaata aaaatatgaa
     1921 attttttata cacaatgatg atgaatctaa ctagatgaat aaatttaatt gtaaataaga
     1981 aagtacttac ttaaatggta ggtgcttttg ttttgccaaa ttatgctata atattgagtg
     2041 agatgagtaa tttgtggggg aattttataa tgtggatatt agtaccaata ataacaatag
     2101 tattacttat aatagctgtt tcttctatgc aatatatttt agttatgata gcttttttgt
     2161 tgattatata ttcttttata gaaaagaaaa tagttatggg tcttgtgtca gttctttttt
     2221 tcacttattc aatttatctt tgtgcaactt gggaagataa atccttgata gcagataata
     2281 aagttgagac tgttaaggca caaagagaaa ctgtagaaag agaaaaagaa atggaaagaa
     2341 gaagaataca agaagaagta gacaaagaaa gatatattga aaaacatgga atggaaatat
     2401 cagaaaatga tttaaaagtt aagctagagg ctttagtacc tcaagaatat aaggggaaga
     2461 aatatgaatt aaaagtagga aaatttaaaa gatatagtat gtattttgat ttaactgtac
     2521 aaaatgaaaa atttagtaat tctgaagaat gtaaaaaatt tgtaaaagaa attgctaatg
     2581 atcttaaaaa aattaaaata agcaaggcgt actttaagtt tcattcaaaa gatgacggcg
     2641 gtatatataa ttctgtttat attgattatt ttagaaacat acaaaataat gtagataatg
     2701 ttgaaaattt agaattcaat gagtttgaac ttaaaaccga agaagaagag aagcgagaac
     2761 aagagaaaat agaacaagaa aaaaacagtt ataataacta cattcaaaac agagtggttg
     2821 acccactaga tagaataaag aaactaaaag agttgctaga ttcaggagca attacatagg
     2881 aagaatataa taaaaagaaa aaagaattat tagaatagat aatatagtaa gcacttacag
     2941 gtatgtaggt gcttttaaat ttacaaagta ttccatttta attttatagt ttagatttta
     3001 tgatataata aaaatataga agttttgcag tgtgcgatat ttgttacaaa gtagggctta
     3061 atacttgaaa tctaagatgt tgagggtgcg tgataagtgt tatcaattgc actattgccc
     3121 cctcactgca attttaagag tattgtatat atgtaggtat tggaaatgct aagtttattt
     3181 tggggtttta gattaactat atggaatgta aaaatttttg acgctatgaa gaaatttact
     3241 acaaaatttg ttttagatta actatatgga atgtaaaaac ttttttcatc ctcccaaacc
     3301 tcttgttcgc tgtcgtttta gattaactat atggaatgta aaaaagaaaa taagtttaaa
     3361 aaaaggtttc attattatat cgttttagat taactatatg gaatgtaaaa ccaattatgt
     3421 acagtttagt tactgtgtta agtcttgttt tagattaact atatggaatg taaaatcctt
     3481 ttataaatat cttctgacat gtgtatatgc ttgttttaga ttaactatat ggaatgtaaa
     3541 tcggctacaa cggtataaat taaatttagc agggtacatt ttagattaac tatatggaat
     3601 gtaaattgct gtgatttcaa taataaaaca attaataatt gtgttttaga ttaactatgt
     3661 ggattcaaaa ttaaataaac aaagaaagca cttacaaata tgtaggtgct tttattctgc
     3721 tcaaaattgg tcggttgggt aaaataatta gaaaaagtta gtaaaaacct attgactgta
     3781 actcgttaca atattgttat taatgtaacg agttacagaa aagaggtgaa taaaatagca
     3841 actaaaagta gggcagagta tatgaaaaat cgtcgaaaag ataaaagagg ttttagtgta
     3901 cttttagaca aagaaaagtt agataaattt gatgaagtgt tagaggaaaa gaatctaacc
     3961 aagaaagaat ggctagaaga aaaaatcgac gaggaactgg aacaaaagga ataaaaaata
     4021 agggtcactc ccaccgacca aagtttgagt aacccctatg acgtatacta tcgtatatca
     4081 attatagtat atgtcattcc ttaaaaaaat caattattaa ggagtgtaat attatgaaaa
     4141 atgaattaat gatgtttgaa ggaaaagaga ttgaagtatt tgaatttgaa ggaagaattt
     4201 tattcaactc aaaagatgta gtaaactgtt tagacataaa aaatgttaat gagaatataa
     4261 gacttatgaa tgaaaagcaa gttgtaaagt taagaaattc ggatatcagt aatactgaca
     4321 tccgaaaact taataatgct ggagaaaatt ttcttactga aagtggagtg tataaattaa
     4381 tctttaaatc aagaaaagaa gaagctgaga gattccaagg ctggataagt gatgaagttt
     4441 taccaagcat aagacaaact ggtgcataca taaataataa tgcaataccg aaaagttgag
     4501 agaaaaagca agtgagattg aaaaattaca gttggtttat aacagtacat ctatgttaaa
     4561 agaattgcta gatgatgcag gctttgacaa taaatcaaaa ctattaacag ctaaaacatt
     4621 atataagaaa gcaggaattg atttaccaat agagataaac gaagaagaac attattttga
     4681 tacaaaacaa atagcatcta aactgaaaat atattctaag agtaataaac cagctcagat
     4741 ggcagtttgt gagattatta aaaagattga tttagaagat ggtgaagtta aaggtgtttg
     4801 ggaaattaat ggttcttgga ctggtactgt aaataaatat acaaagagtg taatagataa
     4861 ggtgagaact tggatagagg aaaataatag acctaccaag attgcaggtg agaagaagaa
     4921 ttattatgta ttttacaaaa ttgagtaaat ttatcagttg tattaaataa tttagtttag
     4981 ttttgggggg attaatacaa tgtgtgagaa tttacttgat aatatgtata ttgaaaagag
     5041 aaaagaagaa tatagaatta aacttttgaa aataagagaa acagatatag atatatataa
     5101 taagttagaa agtatagtat ataaactttc tgagaaaaaa ttagagaaaa ataataaata
     5161 gacatataaa gcacttggat attctactgt ttcaagtgct tgtttggtat aaaatggtat
     5221 aatggagata gagaattata ttaaccaaaa aatggagaaa atagagaaga tgatttagtt
     5281 gacgctttat cagtaggagt acctgtaggt aagacagaat ctctagtacg attagttgga
     5341 gatggaataa gtgatactca aaaaagttta ataaataaaa gagaatttag aaatataact
     5401 caaattggtg gaaatggaaa tgagaaagca tttagtgaag tagaaaactt agttaaatag
     5461 attagaaggt agcaactaga gttggttgtt accttctttt tttatacttt ctctctctaa
     5521 gtattttatt tttcatatct tcgaatacat ctaatgcagt agtataaaat tgtatattat
     5581 ttctataagg tatagctatt ggaagaatgc ccttgttaac taaaaaaata agaagtacaa
     5641 taaataaaag tgtcataatc attctaataa aataattagt gatatctgat aaatcaaatg
     5701 ttaagttaat tgttgtagaa aatacaacag ccataataga tatatacaaa gcaaaaatgg
     5761 ggttactatt tttttgtaac tgtcctttaa gcctagattg ctctatttct atatcttgaa
     5821 tactttttat ggaatccatt tcactataat acttataaac ttttaaataa tattttttat
     5881 acttgtttaa agcttcttca ttatctttaa ggtctttgtc acttaactct gatttaactt
     5941 ccccaaaatc cataaaatcc ctcctacaat gttatataat atactttaat ttatagcata
     6001 tttaagaact tatatcagca atttgtcgaa cgattttagc taaaaataga gtataatgat
     6061 aggcttatta t
//
LOCUS       DAENCV010000183         5981 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.186, whole genome shotgun sequence.
ACCESSION   DAENCV010000183 DAENCV010000000
VERSION     DAENCV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.186"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(19..2289)
                     /gene="slpA"
                     /locus_tag="KQD82_003553"
     CDS             complement(19..2289)
                     /gene="slpA"
                     /locus_tag="KQD82_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG4381992.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFADDTKVETGDQGYTVVQSKYK
                     KAVEQLQKGILDGSITEIKVFFEGTLASTIKVGSELNAADASKLLFTQVDNKLDNLGD
                     GDYVDFLITSPGQGDKITTSKLVALKDLTGASADAIIAGTSSADGVVTNTGAASGSTE
                     TNSAGTKLAMSAIFDTAYTDSSETAVKITIKADMNDTKFGKAGETTYSTGLTFEDGST
                     EKIVKLGDSDIIDITKALKLTVVPGSKATVKFAEKTPSASVQPVITKLRIINAKEETI
                     DIDASSSKTAQDLAKKYVFNKTDLNTLYKVLNGDEADTNGLIEEVSGKYQVVLYPEGK
                     RVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDRIE
                     TAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSKDK
                     LDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAGDD
                     RYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATPIV
                     VVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQAT
                     NAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNSDG
                     KPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQVGK
                     GIATSVINKLKDLLSM"
     gene            complement(2501..4090)
                     /locus_tag="KQD82_003554"
     CDS             complement(2501..4090)
                     /locus_tag="KQD82_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG4381993.1"
                     /translation="MKVNKRVLSIGLAISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4309..5910)
                     /locus_tag="KQD82_003555"
     CDS             complement(4309..5910)
                     /locus_tag="KQD82_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG4381994.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNLLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEDSL"
BASE COUNT         1728 a         1155 c          656 g         2442 t
ORIGIN      
        1 tatattatta tatttatatt acatacttaa taaatctttt aatttgttta taactgaagt
       61 agctatacct ttaccaactt gaactaagtt ttctccatta tctttatcaa gaactttact
      121 tatagataca ctttgatctg aagataaaga atcagtagct aatactatag gtgctggaga
      181 tactaattta tttttatcat tatcagaacc agttaatact ttaccatctt tatctactgg
      241 cttaccatca gaattaagag ttacaccaaa gtttgctgca actggagctg ctgctaaagc
      301 atcaactaat tgatcttctt tagtagaacc atctttagct acaaagaaat taactacttt
      361 tttatcatta tttaagttat cttgataata agaagattct tttataactt ttgcattagt
      421 tgcttgtcta tcatctccac tatatctatc tggagattta cctgtagcat catctattgc
      481 attttcaaca tctttagata cactgttttc tccacctatt atatcaactt gtgaatcatc
      541 taagaaatct tttacatcat catttatagt tttagctttt ccatctacaa ctactattgg
      601 tgtagcatca ccatcagcta aatccatttt accattagca tttcttaatt gagatgcaac
      661 tggagctata ctcatggcat ctgctaatcc tgttcctcca actacaaatg ctttatcatt
      721 atcaagacct acttcatcag ctatttttag agaagtttca tatctatcat ctcctgctaa
      781 tcttgtaact ttaagtccca tatcttttaa ttcattttct acttctttag atatagaatt
      841 aactccacca gctaaataaa ctttctttga agtatttata cctgttgtac tctttatatt
      901 cataactctc tttatttcag cttttacgct tgaatctaat ttatcttttg aagttaataa
      961 taaaggagct ttcttttcag aagctaaagg agaagctaca agtccatcaa ctattgcatt
     1021 tcctccaacc aatactacat tatcaactga atctctaaat atagcatttt catcatcaga
     1081 gttataatat ttttgactta atgctattgc agtttctatt ctatcttctc ctgctacttc
     1141 tatagcattt gaatatccat tattatatgt tcttaaatca tccacataat cttttaagtc
     1201 tttcttctta tctgacttaa gagttaattt aactggtgaa ttttcatcag caattgaagc
     1261 ctttgcagca ctcttagttg taactctttt tccttctgga taaagaacta cttgatattt
     1321 tccactaact tcttctatta atccattagt atctgcttca tctccattta atactttata
     1381 aagagtattt aaatcagttt tattaaatac atatttttta gctaaatctt gtgctgtttt
     1441 actagaacta gcgtcaatat ctattgtttc ttctttagca tttattattc taagctttgt
     1501 tattactggt tgaacactgg cacttggtgt tttttcagca aacttaacag ttgctttact
     1561 tccaggaaca acagtaagtt taagagcttt agttatatct ataatatcac tgtcccctaa
     1621 tttaacaatt ttttctgtag acccatcttc aaatgtaagc ccagttgaat aagttgtctc
     1681 acctgcttta ccaaatttag tatcattcat atctgctttt atagtaatct taaccgcagt
     1741 ttcagatgaa tctgtatatg ctgtgtcaaa aatagctgac attgcaagtt ttgttcctgc
     1801 tgaatttgtc tcagtagaac cactagcagc tccagtattt gtaacaacac catctgctga
     1861 agatgttcca gcaattatag catctgctga agcacctgtt aaatctttca atgcaacaag
     1921 tttacttgta gttattttat ccccttgacc tggagaagtt attaagaaat ctacataatc
     1981 tccatcacct aaattatcta gtttattatc tacttgtgta aacaataatt tacttgcatc
     2041 tgctgcatta agctcagaac ctacttttat agtagatgct aaagttccct caaagaaaac
     2101 tttaatttct gttatacttc catctaatat tcctttttgt aattgttcaa cagctttctt
     2161 atacttgctt tgtaccactg tatatccttg atcaccagtt tcaacttttg tatcatctgc
     2221 aaatacaggt gcagccgaag ctaaaactgt taaacctgac atagctattg ctatattttt
     2281 cttattcatt ccttaaattc ctcccaacat tatattaaaa ttacatctct tattatgtac
     2341 atagaataat catccatata ttcctaatat ttctcacatc tctataataa tccattttta
     2401 ttctttttgc aaggcatttt tttgaaatta ttatttttcc caagcatatg agtttttata
     2461 cccttaaatt tcaatattta ataacttaaa aaatttaatt ttacttattt aaaaattctt
     2521 gagtagcata cacttctcca ttaggcaata tatataatcc aaacccagta tatacaaaat
     2581 cactatttaa catattttca ttatatttat ctgacttttt ccaaactgta aatattacat
     2641 ttgctagttc tctagcatct tttgttgtat aagtgctctt tgtaacaagt ggtacaaaag
     2701 ctatattttc ttcacttctc aacccatatc cagtaaatac ttcaacagat tttttaccat
     2761 ctattacatg tgaaaattgg cctttatctg ccatgtactt agaccaggaa ttagaaagcc
     2821 cttctaagtt tttagaaact tgtaatggct ctttaccttt ttcttttcta tatgaattta
     2881 ccaaattata catttcacta gaaaccattc ttttaaactc actactattc atatcattag
     2941 tcttatattc aagtacattt acttctacac ttattgaggt agtctttcca ccaatatcag
     3001 tagcttttat agtaactttg tatttaccaa ccttatcagt attgatattt cctataagct
     3061 ctggtgtcaa atcgttacca ttgctatcct ttgctactat attaagttta cttatatcaa
     3121 acttatcacc ttgataaata tttaaatttc caacagttat tgttggtgca cttgcatcca
     3181 gtgatgatgc acttaaacat tgttctagtg tcttttcatc tattcctcct agtgctgtga
     3241 ttttatcagc acttctaagt acagacttat cactatttcc atcaaccaat acaataggtg
     3301 catttttagc tatagaagaa ccagcaactg catctactaa ttgataacct ttagaaagat
     3361 aaaattcttt tgctgaacta taaaaatgtt ttataacttt tttattagtc tcaaatctat
     3421 cttctccagc taatctaact gcattagttt ttttaactaa atcattactt attatctcct
     3481 ctgaaccaag agcatagcat tgcacacctt cttttttctc aaatggaaca tttttaccat
     3541 tagttaaaat tataggtgct ccatctcttg atgctactga tgacgcactc atagcatctg
     3601 cttcacctgt ataaccatta gttataaaaa ccttatctat aggttttatt gaagctattt
     3661 cttttgctat aagataactt gtttcaattc tatcattacc acctattctt ttaacttcta
     3721 tccctttttg ctttaattca ttttcaactg attttcctat tgaatcttca gttcctatta
     3781 tataaacctt ctttacatct tttaacattt tttctgtatc tgatggtata ctgtttcttt
     3841 gtgataataa tataggtgct ttaactgttc ctgctaatcc acttgcactt aatccatcag
     3901 ctagtgtatt atctgtattt actaatacta cattttcata tgtttgtttc tgtgcaattt
     3961 tggcagctgt ctcatatcta tctttccctt gtatcttttc tatacttgaa agtgcattga
     4021 tatttggtgc tcctgccatt attaatgata ttgccaatcc tattgataat actctcttat
     4081 ttaccttcat caaactcctc ctactaattt ttttatataa ttcagctaat cataaattac
     4141 atcctcagta gtgattccct caaaatctat aaactactaa aacaaaaaac acctaaaata
     4201 ccataacctg taaagcaata acaaaacaga ttacaaaaag tactttagat gtgattttca
     4261 ttttataata tcagtaaatt aacaaaaaaa caagatttat ggaatttttt acaagctatc
     4321 ttcatttcca tttaaaaact cctgagtagc atgcacttga ccatcagata atatataaag
     4381 cccaaatcca gtactataaa actcatcact aagcatgttt gcattgtatt ctggagactt
     4441 cttccacact tcaaatatag cctttgccaa gtcttttgca tcttgtgtcg tttgaacgtt
     4501 cttagaatct atataaatat atgctatatt ttcctcactt ctcattccaa attcagaaaa
     4561 tacttgtgga gcattttttc catctatata atgtgcaaat acttttttat ccatcatata
     4621 cttagaccat gcatttgcca taccttctag cctactagac actaccaaag gctctttgcc
     4681 tttttctttt ctataggaat tgattaagtt atacatttca gtagatacca tttttttaaa
     4741 ttctgggctg ttgtaatcat gagatttgtc atccaacact tttatttcta ctctttttcc
     4801 tgtacttttt ccccattcgt ctgtagcttt taatgtcaat atgtatgtac ctactctatt
     4861 tgtatctata aacccatcaa cttctattgg taataccttc ccagtgttat ctttagctac
     4921 tatatttaac ttgccagtgt caaacttttc acccttatat acctctgtac ttccaactgt
     4981 aattgttggg ggttgtccat ttgactttga agcacttata cattgttgta ttaccttctc
     5041 atctatttca cctacagagg ttatattctt agctccttct aatacagttt tatcgcttcc
     5101 atcatttact agtaccattg gagagttttt agtaagtggt gcggctgcta ttgcatctac
     5161 taattggtat ccatctgata catagaatcc atctgcacta ttaaaaaagt agtctattac
     5221 gtttttgtta gtttcaaatc ggtcagttcc ttctattctt actgagtttg tatttttaac
     5281 tagaagatta ctcattatct cctctgaacc tatacaatat gattgaatat tttttacatc
     5341 aaaaggcaca ctctttccat ctgtaagtat gattggagct ccatctctag tagctactga
     5401 tgatacactc attgcatctg cttctccact atatgcatta gttaatagta ctttatccac
     5461 tttttttata gttgctattt ctttagctat taaatagctt gtttttagtc tatcttctcc
     5521 accaattctt ttgacttcta tatttttaga atctagttct ttttctactg atttacttat
     5581 agtatcttct gtacctatta tatatgcttt tttgatattt tttagacatc tatttgtgtc
     5641 tgctggtatc ttattttgtt gtgtaaatag tattggagct tttgtagctc ccgataatcc
     5701 acttgcactt aatccgtctg caagtgattt ttctgtattt actaaaatta ctgtttcata
     5761 agtctgttta tcagctatct ttgctgctgt ttcatatcta tctttaccgt atattttttc
     5821 tactactgat gatgcattta cacttttgaa gtttactaag attaatgata ctgctagacc
     5881 taatgataat atttttttgt tcattttcat atccccctga tgatttttat attttatatt
     5941 tatattgtaa ttattttcca tttattttct ctttattttc c
//
LOCUS       DAENCV010000184         5979 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAENCV010000184 DAENCV010000000
VERSION     DAENCV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.187"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(1026..1577)
                     /locus_tag="KQD82_003556"
     CDS             complement(1026..1577)
                     /locus_tag="KQD82_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4381995.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1768..2730
                     /locus_tag="KQD82_003557"
     CDS             1768..2730
                     /locus_tag="KQD82_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG4381996.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIILTLNDSGLKYLSTDLYEEL"
     gene            3025..4869
                     /locus_tag="KQD82_003558"
     CDS             3025..4869
                     /locus_tag="KQD82_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG4381997.1"
                     /translation="MQKRILRNFIFTGKNILNKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICLNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIKEASHAKADYAEI
                     DVQEAKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDMYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5191..5775
                     /locus_tag="KQD82_003559"
     CDS             5191..5775
                     /locus_tag="KQD82_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG4381998.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2345 a          586 c          917 g         2131 t
ORIGIN      
        1 aaaaaataat taccttataa atagattgaa atttatgaat attcatacta taatttaaat
       61 ataaggagat gccctttgaa aataaaaatt aaaaaatatt taatgctact acaataggaa
      121 ctagaactac acttaataaa tatacagaaa ttagatttgg ctcaatataa aatacaaata
      181 tagaatttag gtgtttttta tgaaaaaaat tttatatgct ttatatagtt tcattgttat
      241 tatagctaat tttagattaa aagaaaaaaa ttataacttt attttattag caatcttttt
      301 tatattgctg ttaattcaaa atggagtttt aaaaacaggt tatcaaaata gagctacatt
      361 ttatcaagat aattatttta atggagagga taaaaatgat aagataaaat ttatgactat
      421 aaagattttt atatttttgt cattaccatt atgttttaat atactattca attatttaac
      481 ctcttaaatt atatttagat ataggagtct tatgataaaa gtaagcttat gtgttatttg
      541 tagtaaatat aaagtgcata tagtgataaa acaaaagttt catataaatg aagaaaaaaa
      601 tataaaataa tttatttaac tacttaaaaa taaactaaaa gggataataa aatagctttt
      661 aggttatggt aataaaggta tttaaaaaga gtgcataagc actccaattg tgagaaatac
      721 atacacaaat cattattaca acaatgaaaa atgtacatat aagagacgtt ttgtatttgt
      781 tttaagtgat aatattttaa tactaactaa ataattatca caatagtaaa aaagaaaaag
      841 gagtattttg tactccattt gtcaaaaata taaaactttt atataagctt attataacat
      901 aattgataaa agtgtaaaga tatataatga atttataaag aaaaataaaa taaaaaagat
      961 aaatcctaat caaaggattt atctttttgt atagaaaata attattctat tattaaatgg
     1021 catggctaaa ctggataata atttattatt actgctttca atgttttttc tccttgatta
     1081 tggtatgtat gaattgattc agctgaaaaa caaatagagt cattttctct aagttgataa
     1141 atagagtcat ttacttctaa ttttaattga ccttctataa tcataatata ttcctgtgat
     1201 ttttcagaat gaccaacaga tgtataacta tgaccttctt ctaattccat ttgaaataat
     1261 tcgaagtttc tagttggtgt attgggataa taacaataaa gacgataatc tttattttct
     1321 gaaacttgta catctatatc agtttttgat acaataaaag tttcattttg aggttgttcc
     1381 agtattgcag tatatggaac atttagtcca cttgcaattt tccaaattgt gtttactgtt
     1441 gggtttgagt ctcccttttc aatttgagaa agcattactt tacttacgcc agataattca
     1501 gcaagttgac ctagacttaa gttacgttca ttacgtaatc tatttaaatt tttagcaata
     1561 acagtattaa tactcatagt aactccttaa aatgtaaatt attgacaaat ataatttacg
     1621 aatgtataat ttatttaaca ttatttataa tttacaaatg tataatataa tgaacgttaa
     1681 atacactgta catccgtaat ataaattata ctgcataaag agttgttttt caacggatga
     1741 tagttgagag attaaggggg aattgtaatg cgtgtactta ataatgttac agaggctata
     1801 ggcaatactc atatgttaca tttatccaaa atagcagatg aatttggagt tcaaggtaat
     1861 atttatgcaa agatggaata cttaaatccg ggatttagta aaaaggaccg tgtagcatta
     1921 caaatgatta ccgaagcaga aaatgcaggt ttattagttc caggtcaagc agttgtggaa
     1981 ttaacaagtg gtaatactgg aacaggacta gcaatagctt gtgcatgtaa aggatataaa
     2041 tttatagcgt gtatgtcaaa aggaaattct atggaaagag caagaatgat gaaagcttta
     2101 ggtgctgaag tagttcttgt agaccaaatg cctaattcag ttcatggaca ggtatctggt
     2161 gaagatttgg cacttgtaga aattgaggct caaaaaatag caaaagaaag aaatgcattt
     2221 agagcagacc aatttaattt agaagcttgt aatcatgcac atgaaaagta tacagcagaa
     2281 gaaatgtggg aacaattgga tggaaatatt gatgtatttg tagattttct tggaagtggt
     2341 agtacttttg caggatgttc aaaaacactt aaaaaacata attcttctat caaatgttat
     2401 gttgttgaac ctgaaacagc agcaatttat tcaggtaaag aaataactga ccaagggcat
     2461 aaaatacaag gtggaggata ttctatggat ttgccattag tagacaagaa acttattgat
     2521 ggttatattc aagttacaga cgaggaggca actgatgtag caagaaaatt agctaaatta
     2581 gaaggtgtat ttgcaggatt ttcttcaggg gcaaatgtat gtgcagcaat taagttgtta
     2641 aagagcagtg aaaaaggaaa aaatataatt ttaactctta atgatagtgg tttaaaatat
     2701 ttaagtactg atttatatga agaattgtaa taatagcaca atagttatgc aatgtttttt
     2761 aaattaagta tatagattta aattataaaa agtatctcaa tgtaattttt gaaagttata
     2821 aaggagatac ttttttattt tgtaaatatg gagtatccta cttataagta ttaagctata
     2881 tagatattga tgattagcta tgcaataaaa aagagtatgt gtttttgcat ttatacatca
     2941 taacttggaa taatacatat taatgttcta aatgtattta aaatatatga taaaattaga
     3001 acaaatagat acaaggagat aaaaatgcaa aagagaattt taagaaattt tatatttacg
     3061 ggtaaaaata ttttaaataa acttgttttt ataataaaat ataattttag tacattaatt
     3121 ttatttgaat tatttcataa aggattagcc ttatttttta tatggccatc tattagatat
     3181 atatttgata ttttaataaa aagtttacat atagtctacc taaatatgga taattttata
     3241 aaagttttga ccaatcccat gtcaatagtt ctgataatac tatcagttat aatactagct
     3301 ttttatgcat tttttgaatt tacctctgta attatttgtt taaataaatc gataaaatat
     3361 gaaaaaatag ggttatttga actctttaaa atatcatttg agaactctat aaaattgtta
     3421 tatcctaaaa atatactatt atttatattt gtgatattaa taattccatt agtaaataca
     3481 gttttaattt cagggtttat aggaaaaata caaataccag agtacatact agattatata
     3541 aagtctgata taattttaaa tataatctat atgatttttc ttttaataat atatatttcg
     3601 gttattagat ggatttttag cattcatgag ataactttaa atacagaaaa ttttaaaaga
     3661 gcaagaaaag aaagtgttaa tcttataaag ggaaagatta taagaacaat tatttattca
     3721 ttaatattat ctttagtagt agctattatt ggatatgcga tttatcatag tggaattata
     3781 tttataggca tatggactaa atattgtact gatatggaat ttttgaagaa tgtatttata
     3841 aatagatgta ttgcatttga agaatatttt agattgattt tatcaatatt catttttata
     3901 ttggacattg gatttatctc agcaacttat tatcaatata aaggagtaag tatttctaaa
     3961 aataatatta aaaaagagaa aaaatctata tcaaaaattg tttttaattt agtattaata
     4021 ttattgattt cttatataga gtcagttgca ttttcttcac atataaatgg tatgtttaat
     4081 acgtcatttt cattttatac aacagcaatt gctcatagag caggagctga aatggctcca
     4141 gagaatactt tggcagcaat aaaagaagct tcacatgcta aagctgatta tgcagaaata
     4201 gatgttcaag aagctaaaga tggagaactt attgtacttc atgattcaaa ctttaagaga
     4261 actacaggag tagataaaaa tgtttgggat gtaaattata atgaagtaaa gacatatgat
     4321 gcgggaagct tttattatta tggaaaaggt tattatgatg aaaagtttgt tggggaaaag
     4381 ataccaacac tcaaagaaat gataaaatat tctaaaggta aggtgaaatt attaatagaa
     4441 ataaaactta atggacatga aaagggagac gtagtaaaaa aagttgttca gttaattaaa
     4501 gaaaataatt ttgaaaatca atgtatagtt gcatctatgg ataaaactat attacaaaaa
     4561 gtaaaaaaac tggatgagaa tttattgaca tgctatttta tggcaattgc atatggtgat
     4621 ttttcaaagt taaattatgt tgatatgtat ggaattgaat caacttttgt taataaaaca
     4681 gtagtagaaa agattcataa aatgaacaaa aaaatcttcg tttggactgt taatagtgac
     4741 agtttaattg agaaaatgct tgacctaaat gtagatagta ttattacaga taatccatac
     4801 cttatagaga gtgcaattta ttggaagaaa aatggattta ttagtcaagt gtcagattat
     4861 ttattttaaa tattctataa ttgtattatc cagtaaaaga gatattatta aaatcaataa
     4921 tatctctttt taacataaat aaggttgtta aattaaggtt attcttataa aaattgtgct
     4981 tatcagctat aaacaaggaa taaaagtgaa aaaattgctc tgagattatt acagttaaaa
     5041 gagttttaag atgacagaga gatagattgt caactgtata tttaataaat cttgttattt
     5101 attaaatttt atataagtaa aatatataaa ttaataaatc ttatgctaga atatatatga
     5161 aaaacaatat aaatttggag gattaaaaac atgacagagc gagaaaaaat gttggcagga
     5221 gaactttacg attgtggtga tattgagttg ttaactcaat ggcataaagc aaaagaccta
     5281 gtaagagatt ataaccaaac agattcctcg gatttggaaa aaaaagaaaa aatattaaat
     5341 gagttacttg gtggtaaggg taaaaactta tggattactg caccgtatta tgtagattat
     5401 ggaaataata tttattttgg aaataattgt gaagtcaata tgaattgtac gtttttagat
     5461 gataataaaa ttatcattgg ggataatgca ctaatcgcac caaacgtaca aatatatact
     5521 gcatttcatc caactaatgc tggtgataga tttggtaatg ccaaagaaga tggttcattt
     5581 gaattttgta aaactcaaac agctccagtt actattggag ataatgtttg gattggcggg
     5641 ggtgctatta tcatgccagg tgttactatt ggagataatg tagttatagg tgcaggaagt
     5701 attgttacaa aagatattcc aagtaatatg attgcatacg gaaatccatg ccgtattatt
     5761 cgagaaaata aataaagtta aattttcttg caaaatctca aaaaacacct ttcttacttt
     5821 atacataggg tattttctaa tactttcaca tccatattca tttgttaaat ttttgtggaa
     5881 tgtagttttt atcacttcta tcttacattt ccaccatttt tttataaaag tcattccttt
     5941 atatatttat ttataatagt atatattcta tttgatgtt
//
LOCUS       DAENCV010000185         5952 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAENCV010000185 DAENCV010000000
VERSION     DAENCV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.188"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            167..907
                     /locus_tag="KQD82_003560"
     CDS             167..907
                     /locus_tag="KQD82_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG4381999.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            919..2697
                     /locus_tag="KQD82_003561"
     CDS             919..2697
                     /locus_tag="KQD82_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG4382000.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2864..3781
                     /gene="pfkB"
                     /locus_tag="KQD82_003562"
     CDS             2864..3781
                     /gene="pfkB"
                     /locus_tag="KQD82_003562"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG4382001.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKGGALLITEEEEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            3818..5737
                     /locus_tag="KQD82_003563"
     CDS             3818..5737
                     /locus_tag="KQD82_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG4382002.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         2457 a          546 c         1090 g         1859 t
ORIGIN      
        1 aaattatgta aaatttaggt taattttgta taaagagata cttttatatt ttacaattaa
       61 taaccaaata ctaaaaaata ttttaaataa agaacttaaa attgtgataa aattaattta
      121 tagatattaa aaaaagtata atatatggtt tttaagaggt gctaagatga aaaattttag
      181 agatttaggt gggaacaaaa ctgaggatgg cagaactgta aagaagggat tattttatag
      241 gtctgcaaaa ttatcaaatc taagtgaaaa tgatataaag attttaaaag agttaaatat
      301 taaatatata tttgattata gaagtgatga agaagctcgt aaacatccat caacaataat
      361 atcaaatata aaaaatataa gaataccagc aatgagagag ctagaagaat caggaggaag
      421 ttttggttct attgaagata tgattgatgg attgtttgaa aaagatggtg catttaatat
      481 gttaaataat agttactata atttgcctat aaataatccg tcatataaaa aattagtaga
      541 acttataaga gactattcta atttaccaat attaaatcat tgtacagctg gtaaggatag
      601 aactggtgtg gggagtgcta taattcttat gattttaggg gtatcgagag agaatataat
      661 gaaagattat ctaaaaagta atgattttgc agataaagag atagaaagat ttatagaata
      721 taagcctaaa tttaaggaca ttccaaaaga aaatttgaaa tatatatttg gtgtaaatga
      781 agagtatatg aaaactgcat ttagaagaat tgatgaagaa tacattagtg tagaagcata
      841 tttatatgga gaatttaatt taaataaaga agaaataaga aaattaagaa atcaatatct
      901 agagtaaaag gtgagtttat gagtttaaat ataacagcaa gacagataaa tataataaaa
      961 atattgttaa attcaaaaga tacaattagt ggtattgcct taagtcaaga gataggatgt
     1021 tcttcaaaaa ctatacaaaa tgaaataaag gatattaata aacaacttag aaatggtaaa
     1081 attttgtcaa taagaggagt tggttataaa ctagaaggaa gttttgatga ggtaaatcta
     1141 aatagcaatc tctatgatga tgtagataga gtagaatata taatcagaaa aatattaacc
     1201 ttaagtaatg aagaaaaaaa tactataaaa cttgaagatt tagccgattc tatgtatgta
     1261 agtgtttcaa cagtaaaaaa tgatttaaaa gaagtaaaaa gaatattgga aaagtattca
     1321 atatcagtta aatctaagca taaacaaggt atatgtgttt tggaaagtga agataagata
     1381 ttaaattgta tagttgactt aagcaataag agagataatc aattgagttt aaatgatttt
     1441 ttaaatgatg atataaagga aagaaaacta ttaataaaaa aattattatt agacatatta
     1501 aatgaaaatg gattggtatt aactgatata gaatttaaga gtgtgttaaa taatatatta
     1561 gttatgttaa gcagacataa gtataatgaa aaagaattta tagagaatta tatagaaaat
     1621 tataggagca aaagaagctc aattttagaa aatgaacaaa acaaagaaat tataataaat
     1681 tcaataaaaa cattttgtaa aaatttaaaa ttagctacat caatagatat aagtaatgat
     1741 aaggtatttg aagagttttt atataagcat attagtagtt tatgtaaaaa aataaagtta
     1801 ggtataaatc aatcacctat aatggctcaa gatataaaaa taaaatatcc atttgctttt
     1861 gaactagcta atatagctaa aaagacaata gaaaaagatt tgaagataga aattaatgaa
     1921 gatgaagttg caaacatagc cctacatata ggaggagcag ttgaaagagc atcttataat
     1981 aataaagata aggttttcaa aactataata gtttgtactt caggcatagg cacatcaatg
     2041 ttaataaaag ctaaattaga gaatatattt aaggaaaaat tagaaatact aaagattatt
     2101 ccttcatact tagttgaata tataaaagtt atagatgttg attttgtaat atctactgtt
     2161 cctattgaag taggagatgt gccagtaata aatatatctc caattttaag tgagaaagaa
     2221 attagattaa ttgaaaaata tatagaaaca ggaaatatat acctagattt agatataaaa
     2281 agcctgtttg atagtgaatt gttttttact gatttggtgt ttaaaaggaa agaagaagtt
     2341 atagatttta tggcaagtaa acttgtacaa aagggctata tagatgagga tatgaaaaaa
     2401 tcttactttg agagagaaaa aatagcaaca acagaaatag ggaatatggt aactatacca
     2461 catggagcta ttggaaaaat ttttgataat aaaatagcaa ttggtatatt aaagaagcct
     2521 atttcttggg aaatatcaga tgttcgttta ataataatgt tagcccttga taaagataaa
     2581 atattagatt atgaagtaat attttcaaaa atatataaaa gagtagattc tatagcaaaa
     2641 gttataagta tatgtgaaaa tagtagtttt gaaaaatttg taaatttatt taattagatg
     2701 ggtagtattg tatatacacc tgtaaatttg gataatataa aactaaataa gataaaaacg
     2761 ataaatttct cttttaatta agagaaattt tttgactttc aaggatatta atataaagtt
     2821 aagatataaa cataaagcaa ataaataaaa aagaggtggc aatatgattt atacagtgac
     2881 attaaatcca tcaatagatt atatagttaa gctagatgag ctaaagacag gaagtacaaa
     2941 tcgtgtaaaa gaagaatacg tttatccagg aggaaaaggt ataaatgtat ctcgcatact
     3001 taaggaactt ggaaatgata acacatcttt aggttttata agtggtttta ctggagaata
     3061 tataataaga acattggaag aaaaagaatt gaagacagat tttataaaga taaaaagtgg
     3121 tttttcaaga ataaatgtaa aaataaaggc gttaaaggaa actgagatta atggacaagg
     3181 tccaaatata gatgatgaag acattgatac tttatataaa aaacttgata agctaaatca
     3241 agatgatatt ttaatattgg caggaagtat accatctact ttagatgaaa aactatacga
     3301 aaatataatg gctagattag aaaaaaagaa tataaaagtt gtagtagatg caactaaaaa
     3361 tcttcttctt aatgttttaa aatacaaacc ttttctaatt aagcctaata acgacgaatt
     3421 agaagaacta tttggtgtta aattaaattc aattgaagac atggtaaaat atgctagaag
     3481 gttaaaggaa atgggagcta taaatgtctt ggtatcaatg ggaaaaggtg gagcattgtt
     3541 gattacagag gaagaagaag tgcttataag tgatgttcca aaaggaaaag taaaaaattc
     3601 tgtaggagca ggtgattcaa tggtagcagg atttatatct gggtatttga atactgggaa
     3661 atatgattat gctcttaaat tgggagcagc ttcaggtagt gcaactgctt tttcatatga
     3721 cttagccaaa agagaataca ttgataaatt ggtaaatgag atatctgtaa agaagtttta
     3781 aaatattaaa aaatatatag aaaggtaatt aatatttatg aaaattatag atttattaga
     3841 tgaaaagtca attaagttaa atttaaattc aaaaaccaaa tcagaagcaa tagaagaatt
     3901 agtagatttg gtcgcaaata gtggaaattt aaatgataaa gaaaactaca aaaaagcaat
     3961 tttggctcgt gaagaaatga gtacaacagg aattggagaa ggtgtagcta tacctcatgc
     4021 taaaaattct tcagttacta aagcttgtat agcagcagca gtgtcaaaag aaggtataga
     4081 ttatgaatcg tttgacggaa gtttatcaaa tctatttttt atgatagctg caccagacgg
     4141 agctaacaat actcatcttg aagtattatc aagattatct acaatactta tggatgaaga
     4201 ctttagaaat aaacttataa attcaagctc tgaaaaagaa tttaaggaaa taatagataa
     4261 aaaggaaaga gaaaaattca gtgaagaata tcaagatgag aaagttactg aaaaaaatat
     4321 aatagatact aaagagaatg atgctaataa gtatcctaag gttttagcag taacagcatg
     4381 tccaactgga atagcacata catttatggc agctgaaagt ttaaataaaa tggcagaaaa
     4441 caaaggtgta tcaataaaag tagaaactaa tggttcagca ggtgttaaaa ataagcttac
     4501 aaaagatgaa atagaaaatg caacatgtat aatagtggca gcagataaaa atgttgagat
     4561 ggctagattt aatggcaaaa aagtcataaa aactaaggtg gctgatggta tacacaaagc
     4621 ggaagaactt atagacaagg ctgtaaatgg agatgctcca atttaccatg gaggagatgg
     4681 aagtcacaac gaatcaaatg aagaaagtga aagtggattt agaaaagtat acaaacactt
     4741 aatgaatggt gtatctaata tgttaccatt tgttattggt ggagggatac ttatagcgat
     4801 agccttttta cttgatgatt atactattaa cccttcaaac tttggttcaa atacacctat
     4861 agcagctttt tttaaaggaa taggagataa agcttttgga tttatgttac ctgtacttgc
     4921 tggatatata gcatactcta tatctgatag accagcgttt gttgtaggat ttgtaggagg
     4981 tgctctagca ggtgatggtg gttcaggatt tttaggtgca ctacttgcag gttttatagc
     5041 aggttattta gtagaagggc ttaagaaaat atttagtgta ctaccagctt cattggaagg
     5101 aataaaacca gtattgttat atcctttact tggaacactt ttgatgggaa ttataatgac
     5161 atttttagta atacctccag ttacagcaat taataatgca atggttggat ttttaaatgg
     5221 tttaggtgga acttcaaaaa tatttttagg attagtatta ggtggaatga tggctgtgga
     5281 tatgggtgga cctgtaaata aagcagcata tgtatttgga gttgcatcac ttgaatcagg
     5341 gcagtttgaa ataatggcag cagtaatggc tggtggtatg gttccgccac ttgctatagc
     5401 tcttgcaact acatttttta agaatagatt tactaaagaa gaaagagatt ctggtaaagt
     5461 taattatgta atgggattat catttgttac tgaaggagcg ataccttttg cagctggaga
     5521 cccacttcat gttataccag cttgtgttgg aggttcagca gtggcaggag cacttagtat
     5581 gttgttcaac gcagctcttc gtgcacctca tggaggagta tttgttatac cagtggttac
     5641 ccatccattt gcatatatat tagctatagc tgtaggcgct ttggttggaa tgatgctttt
     5701 agcattactt aaaaaaccat taaatcaaga agtatagcaa ttaaaggata ttcaagaact
     5761 taaaatatga tgttttataa aaattaatat attgctgaat tattttaaaa taaggatttt
     5821 atgaggctgt tatataacga tttgtaattg ttatcttgac agtcttttat ttttatataa
     5881 gtaaaataag tgtgttttgc tttatttgta tttaaattgt tttcaagtta gaacataaag
     5941 aaaattatat at
//
LOCUS       DAENCV010000186         5941 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.189, whole genome shotgun sequence.
ACCESSION   DAENCV010000186 DAENCV010000000
VERSION     DAENCV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.189"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            109..1737
                     /gene="aspD"
                     /locus_tag="KQD82_003564"
     CDS             109..1737
                     /gene="aspD"
                     /locus_tag="KQD82_003564"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG4382003.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEGLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1858..2853
                     /locus_tag="KQD82_003565"
     CDS             1858..2853
                     /locus_tag="KQD82_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG4382004.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3042..3425)
                     /locus_tag="KQD82_003566"
                     /pseudo
     CDS             complement(3042..3425)
                     /locus_tag="KQD82_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3651..3748
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3890..4385
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3890..3918
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4671..4829)
                     /locus_tag="KQD82_003567"
     CDS             complement(4671..4829)
                     /locus_tag="KQD82_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382005.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5188..>5941)
                     /locus_tag="KQD82_003568"
     CDS             complement(5188..>5941)
                     /locus_tag="KQD82_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG4382006.1"
                     /translation="DKLVNDTNSTRLGGVDRYDTNKKIVNKFYNGAKEFYIASGTDLV
                     YALVASPMAKNNPIVLVDIGSNKDILKNAAKVTGIGNLSDKIMLECENTINNISLPSS
                     NKNIICGLKIDFDSKHIIWQDKGSYLECYDDNLEYYTEKYKDPTVPINIENTSTQNLD
                     VTKLKVYGEANSTLYMGQYAKIKMDFNSFGVLKPGEKCTIYLDIISLDPSRSMTLTFS
                     YVGDKENYKYEQGLASRILFYNPDGEDFFEYK"
BASE COUNT         2108 a          840 c          827 g         2166 t
ORIGIN      
        1 atactttact ttttctataa acctattata attaaagtat aatcattttt gcaacttgtt
       61 tattttatta taaaaatgtg aaacttatta cataaggagg aatttaacat gaaaaatcat
      121 acttataaag aaatcaaaaa catatatgga aaaataagcc cttttgaatt taaggacaaa
      181 ttaatagata ttgcaaaata cactgcaaaa gaaaataaca gagaattact tgatgcaggt
      241 cgtggaaatc caaactggac ttgttcaact gctagagaag ccttttttac ttttggtcat
      301 ttcgctatta ctgaaactcg ttctaattgg gatttaggtc atttggctgg aatgcctcaa
      361 aaaaagggta ttaaagaaag attttttaaa tttataaatg aaaatataga tatgcctgga
      421 gcatatttag ctagagatat tataaacttt ggtataaatg aacttggatt tgatggagat
      481 gaatttgttc atgaactagc tgatggaatt attggtgata actatccact tccagataga
      541 atgttacctc atatggaaaa aatagtacac gattacctag ttcaagaaat gaaatatgat
      601 atatcaggta aatatggtga tgtagaaatt tttgctgttg aaggtgctac agctgctatg
      661 tgctatatat ttgattcatt aatggcaaat gaacttctaa aaaaaggaga tacaatagcc
      721 ttaatgactc caatatttac accttatctt gaaataccta accttcctcg ttacgacttt
      781 aaagtggtaa atataaatgc taatgaagtt gatgaaaaag gtgctcacac atggcaatat
      841 acaaaaaaag aattagagaa acttcgtgac aaatctatta aagctttatt cgttgttaat
      901 cctaataatc ctgcttctat agctatggat gaaacatctt gtaataatct aatagatgta
      961 attgaaaatt ataataaaga tttaatgata atatcagatg atgtatatgg aacttttgta
     1021 gaagattttt catccttaat gtcaaaacta ccttacaaca ctgttggagt atattcttat
     1081 tcaaaatatt ttggagttac aggatggaga cttggtacat ttgcccttca taagaaaaat
     1141 gtttttgata agaaaataaa tgatttaact ggagaattaa aaaaatcagt agataagcgt
     1201 tacagtgata tgagtcttaa cccttctagc ctttcattta tggaaagagt tgttgctgat
     1261 agcagacttg ttgctctcaa ccatactgct ggtctttcta ctccacagca agtacaaatg
     1321 gcgtttttct ctgcttttgc attgattgac aaagtagatg cctacaaaaa actcaatatg
     1381 agtatctgcc atagacgtca aaaactatta tttgaagctt tagagcttcc aattaatgaa
     1441 aacaaaaaca atgctgcata ttacactcaa tttgatattg aagaatgggc taaattaaac
     1501 tacggagaag gcttatttaa atttataagt aaaaatgcat ctccagtaga tgttctttat
     1561 aaattagcta atgactattc agtagtttta cttagtggaa atggattcta tggtcctgaa
     1621 tggtcaatta gaatatctct agcaaatcta tatgatgaag cttatactaa gataggaaaa
     1681 gccataagag agatattaaa tggttatatc gttgaatggc aaaaaattaa gaaataatta
     1741 tattacactg aaatatctaa attatatgaa taaaagagtt tatgtagacc ttaactactt
     1801 tttttgatat aatcctatcg gaattataaa ttttatttat gaaaggcaga gaatagtatg
     1861 agtattaaga aaaaagttgc catagttttt actggtggaa ctatatctat gacagtagat
     1921 gataaagtcg gagcagctat accaacttta tcaggtgaac aaatactatc aatggttacc
     1981 aacattgata aagttgcaga tgttgaagtt tttaattttg atgaaattcc aggacctcac
     2041 ataacacctt atagaatgat ggaattgaaa aactatgtaa atgatttatt agcaagaaaa
     2101 gatattacag gtgttgtaat aacacatggt acagatagtt tagaggaaac agcttatttt
     2161 ctagacctta caattgacaa catcaaacct gtaatagtca caggtgctat gagaagtagt
     2221 tctgaacttg gatatgatgg ttcaagtaat ttatctgcat cagtttgtac tgcagtatcc
     2281 aaagatgcta tgggaaaagg agtccttgta gttttaaaca atgaagtatt attagcttct
     2341 gaagctacta aaacaaatac tctttctcta aatacattta aatctctaac tagtggccca
     2401 ttgggaataa ttgactgcaa cgaacttgtc cttacaagag atattgtaaa tagaacaatt
     2461 atagatactg ataaggtaga atctaaagtt gccttattta aagcatatgt aggagaaaat
     2521 gctgacttta taagatttgc tgttgatagt ggttataaag gtatagttat tgaggcaatg
     2581 ggcagaggta atattcctcc tcaaatgctt tctggagttg aatatgctag agaaaaagga
     2641 cttcctgtag ttatagtttc tagatgtcat tcaggtagag tattcgatag ttatggttac
     2701 tttggttctg gtagagattt aaaaaatctt ggttgtatat ttggtgggga cttaccaggt
     2761 caaaaagcta gaataaaact tatcttagca cttggaaaaa ctgataacct tgatgaaatc
     2821 aaagatttct ttgaaaaagg aatctattgt taattctaat aacttctatt taattattta
     2881 ttgatatttc aacattttta aatcaaaatc taatacttat gtcagttaaa tgtcagcaaa
     2941 atattttatc cacaattttt acacaaattt tccacaactt atccccaata ttatccacaa
     3001 gaaaaaaggt agcacttata ttagtagcta ccttttattt attaatcttc atctttccag
     3061 ctttttatcc aatttcctat ttctattatt ttttcttcag ttatttcttc tccatcatgt
     3121 aattttgaaa ttagttctga taatggattg ttatgtagac ctccaaaaat tctttttccc
     3181 tctattttga gatattcttt ctcttttatt aatactctat agtgtacatg tttcccaatt
     3241 ctctgcgata tgaaaaaacc ttttttagtt aatctagcta aaagtgtaat tgtagttgta
     3301 tgcttccaag catatgtttt ttctatttaa tctgcaacat cttttgatat tacagtttta
     3361 taaccagtat tccatatgta tttcataatt tttaattctg catctggtaa ttttttattc
     3421 aacataatat attccttctt tcaatttatg tcacttcctc aaacttatct agcttaaatt
     3481 aataatagga gctttttatc acaaaaacta caggtaaatt tttctatata ttctacgtat
     3541 ttagtctgtt tttaagtgtg aaattacgtt catacctata aatttattca attttcaaac
     3601 aatcttgttg aataataact aattctattg tattatttag ttatagaata aaactaaatc
     3661 ggcaaaacta gagaaatcta gtgacgcaaa gctataggga ctaagactta tataaatatc
     3721 ttatgagtta tgttagccag ttgccaaaga gatattgttc tttttgtttt tatgaaagtt
     3781 ttttaggggg ataataattt atatgtttag agataaaatg gataaatgta cacatatatt
     3841 gactgcttat attagcagtt cgtatgatta ttgtaatttt ttggatacaa tttacattcc
     3901 atatagttaa tataaaactt gtgtttattg tgatagtgcc gaacaggtgt taatacgatt
     3961 tacattccat atagttaata taaagctcta atttaatagc tttattttta gcaaaatacc
     4021 caaatttaca ttccatatag ttaatataaa accggttccc tccgaagttc atagagattc
     4081 ttatcagcat gatttacatt ccatatagtt aatataaaac aaatgctgaa agataattcg
     4141 gttggggatt taatgaaatt tacattccat atagttaatg taaaactaaa tagagcagat
     4201 gaggcttttc aagaagccga tagatttaca ttccatatag ttaatataaa actctgatgt
     4261 tgtgagattt gaaaaaaacg gtaaattgaa tttacattcc atatagttaa tataaaacgc
     4321 tgataaagtt attaaaagta aaattgctga aatactattt acattccata tagttaatat
     4381 aaaaccccaa aataaattga gtatttccaa tgcctacaca tatacaaatc tctcaaattt
     4441 gcagtgaacc atgagtagtg caattgataa cacttatcac aagtcctcaa tgccttatat
     4501 ttcaactgtt aagccttaat ttcttcaaaa aatcgaacac tgcaaaattt ctatgacttt
     4561 attatatcat aaaaatatta tttttgaata tctgtaccaa tttgtgatat aataaaagca
     4621 agaagaacta caatctattt tgctgtagag tggagttcat aatttagaaa ttacttagtt
     4681 ttatggaatc tgatttttaa atcaaattcc caaccactct tagttgccgc tttgagtggt
     4741 tttttacgct ttctaaatag tttgctagct atataaacta ttaagctagc agatagactt
     4801 gctagtatgc cttgtaaaaa attatccatg tatattcacc tcccttctta gcgtcgggag
     4861 gataattttt tgatacatga actccactct ataaattgta gattacatct tcttgctaaa
     4921 aatattataa catataattc ttacatattt tacctattta ttacttttta cattataata
     4981 atattctttt aatcaaaaaa acacctacta ttaaagtaaa tgtttttaag tatttttaat
     5041 tttaaaatcc atatagttca tatgaaacta aatataataa ctcaacatat gagcaatatc
     5101 gagacattta cataccacat agttaatata aaacgaaaat aaaaaaatta aacttataaa
     5161 acccttatta atttacatac ccactagtta tttatattca aaaaaatctt ctccatctgg
     5221 gttataaaat aatattctac ttgccaatcc ttgttcatat ttataatttt ctttatcacc
     5281 tacataagaa aatgttaatg tcatgcttct tgatggatct aatgaaatta tatctaaata
     5341 aatagtacat ttttctcctg gctttaatac accgaaacta ttaaaatcca tttttatttt
     5401 agcatattgc cccatatata atgttgaatt agcttctcca taaactttta attttgttac
     5461 atctaaattt tgtgtacttg tattttctat atttatagga actgttgggt ctttatattt
     5521 ttcagtataa tattcaaggt tatcatcata acattcaaga taacttcctt tatcttgcca
     5581 aattatgtgc tttgaatcaa aatcaatctt taatccacat attatatttt tatttgaaga
     5641 cggaagacta atattattga tggtattttc acactctagc ataattttat cacttagatt
     5701 tcctatccca gtcacttttg cagcattttt aagtatatct ttattacttc caatatcaac
     5761 tagtacaata ggattatttt tagccattgg tgaagcaact aaagcataaa ctaaatcagt
     5821 accactagct atataaaatt cttttgcgcc attatagaac ttatttacta tttttttatt
     5881 agtatcatat ctatctactc cacctaatct agttgaatta gtatcattaa ctagtttgtc
     5941 a
//
LOCUS       DAENCV010000187         5933 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAENCV010000187 DAENCV010000000
VERSION     DAENCV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.190"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            199..1524
                     /gene="cdeA"
                     /locus_tag="KQD82_003569"
     CDS             199..1524
                     /gene="cdeA"
                     /locus_tag="KQD82_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG4382007.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1619..2413)
                     /locus_tag="KQD82_003570"
     CDS             complement(1619..2413)
                     /locus_tag="KQD82_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382008.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2414..3289)
                     /locus_tag="KQD82_003571"
     CDS             complement(2414..3289)
                     /locus_tag="KQD82_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382009.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3293..3661)
                     /locus_tag="KQD82_003572"
     CDS             complement(3293..3661)
                     /locus_tag="KQD82_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4382010.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3844..4503)
                     /gene="deoC"
                     /locus_tag="KQD82_003573"
     CDS             complement(3844..4503)
                     /gene="deoC"
                     /locus_tag="KQD82_003573"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG4382011.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIEDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4539..5726)
                     /gene="murJ"
                     /locus_tag="KQD82_003574"
     CDS             complement(4539..5726)
                     /gene="murJ"
                     /locus_tag="KQD82_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG4382012.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5803..>5933)
                     /locus_tag="KQD82_003575"
     CDS             complement(5803..>5933)
                     /locus_tag="KQD82_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG4382013.1"
                     /translation="EVKNLNIREWICPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         1972 a          930 c          652 g         2379 t
ORIGIN      
        1 acatatattt ctaaagattt tcttaagaaa cagttagatt ttactaatat aagtttatta
       61 attagcatta tactaattta tcttaatatg attttttgtt gattttaagc tgtttatcgt
      121 ataaataaaa tttttcttga ctatatagtt actatatagc ttattatata tagagagtaa
      181 taaaaggagg taaaatttat ggaaaattta tttacaagaa aattcactac ttttgaattt
      241 ctaaaatttg tttctcctgc aattatatcc atgatattta tatctttgta cacaataata
      301 gatggcatct ttgtatcgac attagttggt tctgatgctc ttgctagtat aaatattgta
      361 ctacctataa ttaaccttgt ttgtggattt ggaataatga tggcaactgg tggaggtgct
      421 atcgtttcta tacgtatggg tgaaaataga caggatgaag ccaactctac attttctttt
      481 atagttttgt tttcattgat tgttgggatt ttattcacag taatctcata tttctttatc
      541 aaagaaatat ctattttgct tggtgcaaca gataagttat taccatattg tataacttat
      601 ggtaaagtta tgattttatg tacaccattt tatattttaa aatttatatt tgagtacttt
      661 gcaagaactg atggaaattc taaatttagt ttatttctat cagtcattgg tggtgtaaca
      721 aatataatat tggattatgt atttattaaa tattttggaa tgggtctttt aggagctgca
      781 gttgcaactg ctataggtat tattttaact tgtgttttag gtattattta cttcttatct
      841 aataaatcta cactaaaatt aagaaaacca aaaaccgatt ttagacttat aagagatact
      901 atgataaacg gttcttctga aatggttaca gaattatcta caggaattac aacattctta
      961 tttaatgtag tagctttaaa attagcagga gaaaatggac ttgctgctct tactatagta
     1021 ttgtatgctc attttttaat gacatcagtc tatctaggat tcgctgctgg agtgtctcca
     1081 ttaataagct ataattttgg tgctgaaaac agtgataaat taaaagaaac atttaaacat
     1141 tctctaaaat ttatatttgt ttcttctctt ttagtgttta ttattgcttt agtatttgca
     1201 ccatttattg ttagggtctt tgtaagtcca gataacacag tatttaaact agccttacaa
     1261 ggattaaaaa tatttgcatt tgcttttttg tttgttggta taaatatatt tgcatcagga
     1321 ttttttacag catttcacaa tggaaaaatt tcagctatta tatcttttag tcgtgccttt
     1381 gtttttataa tcataggaat cataattctt cctcctatgt taaacatgac tggattatgg
     1441 cttacagttc catttgctga ggttataacc atatttatat ctattctatt tataaaaaaa
     1501 tataaatgta gatataagta ttaaaaatta atacttaaaa tcaacattta ttaaaactaa
     1561 taaacctgct ttaaaataca aatttacaaa tttgattttt aagcaggttt tattttagtt
     1621 ataataagtc atttgcatac tctttaattg gtgcttttct aagtaataaa attataaata
     1681 ttatgcaaat aactataatt cctacctttg ttccaaataa aataaaagga ttctgaaatg
     1741 aataaataat tttttctaaa tacagtagca ttttatgcat atttttagtt gcaaaattaa
     1801 ctatataaat taccattaat atcggtaatc ctatcaatat agtaatacta gtaacttttc
     1861 taactctata tgtaaaagct ccaagtaaca ctcctataaa acctatacat atttcattta
     1921 tacatatttg tacaaaataa tttaaataag ttagtggcat acttgatgtt gaaggatcta
     1981 tcagtgctga tatagatgtc caaatcatat ctctctttag taaaactgat agttctatac
     2041 tgtaacctgt tataatttct ataaataact tcgataaaaa aatcaaaact atacctaaaa
     2101 tagaaatcac aaagcttaaa ataattaaat ctatgacaat agcttttata caactttttc
     2161 tatctgctct tatacttaaa gctcctgaaa agtctttatt tgctatattt actccataca
     2221 caaatagcag tatagttata tatgttaaaa taggattcac aatcgtggat aaattagttt
     2281 tagatgcttc tccaaaaatc ttgagaataa acactggcaa aatattcata acaattccca
     2341 caatcataaa tataattata tacaatttag tatttttatt aaaaaatttt atatatggct
     2401 ttaattcatt caattacaat tcctccttct tagtcatgtt aacaaacata tcttgtagcc
     2461 ctatgtatcc tacatcaata tttgaatttt taagtatttt ttcatcactt tcactaaaat
     2521 caccatagta aaaataagca actgcatttc caaatgcctt tttaggtttt atatttttta
     2581 tacattctaa tttttctaac tcttctttat ttccagtaat ataatgagct ttttgtttta
     2641 tgacatcaat ttcttcatct ataataattt ttccctcatt taatattatt acatgctcca
     2701 gtaaatcatc tatttcatca atcaaatgag ttgatattat tattgtcctt ggatttttta
     2761 tataactatc taaaattatg ttataaaact cttgtctatt tactgcatcc aaaccaattg
     2821 ttggctcatc aaatatggtg atagctgaat tagaacaaat acctattata tttgaaagta
     2881 tagttttcat acctcttgac agttgtttat actttttttt agtatttaaa tcaaaatatt
     2941 tgcataattc tttttctaag cctttatcat aacttttata aaaactagaa gaaaattcaa
     3001 atatctgacc tactttaaaa tctggactat aaaattcttt ttccctgaca atacaaactt
     3061 cttccaaaac ttttatatct tcttttgggt tctttcctaa tatttctatc tcaccttcat
     3121 tttgaattaa ctgatttacc attatattta ataatgttgt ttttccagct ccattcttac
     3181 ctaataaacc atatatctta tttttttcaa agcttagaga catattatta aaaatatttt
     3241 gcttcttaaa actgtgagat aaattttcta tcttaataga actactcata tattaatcct
     3301 ccttgaaatt aattataata tcaaccaatt cttctctatt tatttctaat ttgttagctt
     3361 cttgaagtag gttttttaca aaattatgtt taaaattttc ttttctagct tcttttataa
     3421 tagtttttgc accatctgat acaaacatcc ctatccccct cttcttatat aaaatatttt
     3481 tatcaaccaa tatatttata ccttttaata ctgtagctgg attaatttta taaagctttg
     3541 aaagctctgt tgttgatgga acctgttctt cttcttttat tccatttgat agtatctcat
     3601 cttcaattgc tcgtgctatc tgaataaata taggttcttc attatttaat actaatttca
     3661 taggttcctc ctttctccat cagttagtta ctcaactaat taactatata acttataata
     3721 tcttgaaaaa tattttctgt caatactaaa taaaaaacag agggaaaatt ccctctgttt
     3781 tttgttcttt atatttataa ttaaatttta atttttgtaa tcctatccca taagttaaaa
     3841 actttaataa tctgttgtat cttcattttt taatatttta catattgagc tagttcctaa
     3901 cctttctgct cctgcctcta taaatttttc tgcatcagaa attgatttaa ctccccctgc
     3961 agcttttatt tttacatttt ttccaacatg ctctttcata agctttatat cttctaaagt
     4021 agctccacct gttcccattc cagtagatgt ttttataaaa tctgaacctg acattgttac
     4081 tatttcacac atttttattt tttcatcttc atctaaataa catgtctcta taatgacttt
     4141 taatacttta tcttctatag ctttttttat ttcttttatt tcttttccta tattatcata
     4201 atctttattt tttatatctg aaatgtttac aaccatatca acttcatctg ctccattttc
     4261 aatagcatct tttgcttcaa atacttttgt agcagtagtt tgatatccca aagggaaccc
     4321 tatcactgta catatcttta ttttaccttt tagatattct gatgctcttt tcacatatga
     4381 tggtggtatg caaactgatg ctacactcat atctacagct tcatcacata aaattttaat
     4441 atcttcccat gtagttgtag cttttaaaat agtatggtct actgttttta atatatgttt
     4501 cataaaatcc tcctaaattt ataattaata taattagatt acttttttaa atgcaatttt
     4561 ctctttgcaa tattcagtat catatcaacc tcttcaactt taaaaatagt cattagtagt
     4621 gtataaattc cacctccaac tataactgaa atagctagtg atacaaactc attaaatgtt
     4681 cctactccca aaattccaaa tataaattta tacgtaaagt atgataaaac acccataatt
     4741 aaagatgcaa ctaccgattt caaagttgct tttattattt tatcttgtcc aaaatatcca
     4801 acttttctct ttaggttcaa gaagagtaac aatatacatg caattgatga tgaactagtt
     4861 gcaagagcaa gaccaccatg agccattggt tttattaaaa ctaagtccaa tactatattt
     4921 acccctacag atataatccc atttaccata ggtgtttttg tgtcttgtaa tgaataaaaa
     4981 acctttccca aaatatctct taagccaaat gcagtcatac caactgcata aaaaattaag
     5041 gcagttgctg tcatttgggt tgccctcgca tcaaaagcac ctctctcaaa aatgattcta
     5101 acaacaggag ttgcaaaaaa aatagaagca actgaaatag gaatcataga aattattatc
     5161 atatttatac tagatttaac tgaactagta aacttctttt gattattttc tgcacttaac
     5221 ttagataaca taggatacat aacagatact attgaagctg taaatgtccc tgtaacaaaa
     5281 ccatttaatc tatctgcata agtaagtgct ggaatgcttc ctttaactaa tgttgaggct
     5341 aaagttgtat ctaccaatga gttcacttga ttaacagcaa cacctataaa tactggtgaa
     5401 agtaatgcta gtaatttaat aaggctatca tctttaaagt ttaagtagta taagtatttg
     5461 tagttcgttt ttttaacaaa aaacatgtaa aagagcagtt gaactaccat agcaaccaca
     5521 gcaccaattg gaagtatgta tggtccaaat atggtactta acattattga aataattatt
     5581 actatattat agggtatact tccaaatcca actacaataa agttctcttt aatttgtaaa
     5641 aatgctgaca taacacttgt aatacctatg aatattattc ctgttctgaa cctcccatga
     5701 ctaaagtcac agggttcctg cttcatagaa ttttgcatac taaaatatgt cttactaatt
     5761 ctccacaggc tatccccgta gttcctacgg ttcttacata cgttatttat tttgaattgt
     5821 tattagtctt aatccttctt ttaatatatt tctacttgca tttatatctc tatcgtgagt
     5881 ttcattacaa ctaggacaaa tccattctct tatatttaag ttcttaactt cct
//
LOCUS       DAENCV010000188         5788 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAENCV010000188 DAENCV010000000
VERSION     DAENCV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.191"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..821
                     /locus_tag="KQD82_003576"
     CDS             <1..821
                     /locus_tag="KQD82_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4382014.1"
                     /translation="MEYINQKSIKLVDIVENDIISYLIKLEKDNVSIATIARMISSIK
                     SFHDYLFLNHICANNPAKDIKKPKIKKENINILTEEEIERLLNFPKLTTPKLIRDKAI
                     FEVLYGTGIKVSELVEMNIEDIDLDIDYIYCNSCCKNQRVIPLCDITKLYLEKYLKEA
                     RPKMAVEGEKSLFVSSLGQRFTRQGLWKVIKKYSNLANIDKNINPTMLRHSFAIHLLN
                     EGANIAVVSKILGNVNLSSLQVYLNHIDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            863..2035
                     /locus_tag="KQD82_003577"
     CDS             863..2035
                     /locus_tag="KQD82_003577"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG4382015.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            2082..2894
                     /locus_tag="KQD82_003578"
     CDS             2082..2894
                     /locus_tag="KQD82_003578"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG4382016.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            3098..4423
                     /locus_tag="KQD82_003579"
     CDS             3098..4423
                     /locus_tag="KQD82_003579"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG4382017.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            4626..5705
                     /gene="mltG"
                     /locus_tag="KQD82_003580"
     CDS             4626..5705
                     /gene="mltG"
                     /locus_tag="KQD82_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG4382018.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         2349 a          600 c         1068 g         1771 t
ORIGIN      
        1 atatggaata tataaatcaa aaaagcatta aactagtaga tatagtagaa aatgatatta
       61 taagttactt aataaagttg gaaaaagata atgtatcaat agctacaata gcgaggatga
      121 tatcctctat aaaatctttt catgattatt tatttttaaa tcatatatgt gcaaataatc
      181 ctgcaaagga tataaaaaaa cctaaaataa aaaaagaaaa tataaatata cttacagaag
      241 aagaaattga aagactatta aattttccaa agttaacaac gcctaaattg attagggaca
      301 aggctatttt tgaagtatta tatggtactg gtataaaagt atctgaatta gtagagatga
      361 atatagagga tattgaccta gatattgatt atatatattg taattcatgt tgtaaaaatc
      421 aaagagtgat accactttgt gatattacaa aactttactt agaaaagtat ctaaaagaag
      481 caagacctaa aatggctgta gaaggtgaaa aatcattatt cgtaagttca ttaggtcaaa
      541 ggtttacacg acaaggtctt tggaaagtca taaaaaaata ttctaatcta gccaatatag
      601 ataaaaatat aaatcctaca atgcttagac attcatttgc tattcatttg ttaaatgaag
      661 gtgctaacat tgctgttgta agtaaaattt taggaaatgt taatttatct agtttgcaag
      721 tatatttaaa tcatatagat aaaaacgtga gaagagaaat aaaggagaaa catcctagaa
      781 atgatgttga tgtagaatta caaaaagcag aagctaaata gactatatag caaattttat
      841 aatttagaag gagcatattt atatgagcag agtaatttgg attgtaattg atagtgttgg
      901 gataggagca ttgcctgatg ctgaaaaatt tggtgatagt aaggatgtaa gtacccttgg
      961 aaatatattt aaggaatatc cagatattca gattcctaat atgagaaatt taggtatagg
     1021 aaacatagat ggtatagatt tttttgaaag tataaaagaa ccaataggtt gttttggaaa
     1081 gtgtaaggaa atgtctcagg gaaaagatac tactacaggt cattgggaaa tgactggtat
     1141 aatagttgac aaaccattta agacatttga acatggattc tctaaggaga taatagaaga
     1201 gtttgagaaa aaaactggaa gaaaagtagt aggaaataaa ccagcttcag gaactgtaat
     1261 aatagatgag tatggagagc atcaaataaa aactggagat gtaatagtat atacatcagc
     1321 tgatagtgta tttcagattg ctgctaatga agaagtaatt cccctagaag aactctataa
     1381 tatgtgcaaa atcgctaggg aaataatgat gggggataat gcagtagcta gagtaattgc
     1441 tagaccattc ataggtaaga aaaaaggtga gtttgtgagg acttcaaata gaagagatta
     1501 ttcactagac ccatttgaac caacagttct tgataatata aaagaatcag gacttgatgt
     1561 tttagcagtt ggaaaaatag aagatatttt taatggaaaa ggaattacag atgctatcca
     1621 cactaaaagc aatatggatg gagtggatga aactttaaat tatatgaaac aagataataa
     1681 aggtctaata tattcaaatc tcgttgattt tgattctaaa tatggtcata ggagagaccc
     1741 agaaggatat aagaaagctc ttgaagagtt tgatagtaga cttcctgaaa taatggctaa
     1801 tatgagagaa gatgatattt taataatcaa tgcagaccat ggaaatgacc caacatataa
     1861 aggtacagac catacaagag aatatatacc tgttatgatt tatggcaata agattaaaaa
     1921 aggttttaat ttaggagtaa aagatacttt tgctgatatt ggagcaactg ttgcagatat
     1981 actaaatgta aagttgccta aacatgggtc aagctttaaa gaagatttat tttaaactat
     2041 aaaatgataa aaacttagct taataggatg gagagattgg aatgtttgaa aagatagcac
     2101 aaagtagtaa atttataaat tctaaaagta atataaaacc taagattggt ttgatattag
     2161 gttctggact aggtgattta gcaaatgata tagaagaccc tgtcacaatt aaatatagtg
     2221 aaattccaaa cttccctgta tcaacagtag caggacatgc tggacaactt gtaattggaa
     2281 aacttgaagg aaaagaagtt attgctatgc aaggtagatt tcattactat gaaggatatt
     2341 ctcagaaaga agctactttt ccagtaaggg ttatgaaaga attaggagtc gagattctta
     2401 tagtaactaa cgctgctgga ggcgtaaata aagaatttaa agcaggagat ttgatgctta
     2461 taagagacca tataaacttt agtggaagta atccattagt aggaaaaaat gatgacagat
     2521 ttggtgctag atttccagat atgtcagatg catattcttc taaatatgta gatgtagtta
     2581 aaaactgcgc taaagaatgt aatatagatg tcaaagaagg cgtttatatg ttttttagtg
     2641 gtccaaacta tgaaacacca gcagaagtga gaatggcaca aattttggga gctgatgctg
     2701 taggtatgtc tactgttcca gaagtcatag tggcttctca ctctaatata ggagttattg
     2761 gtatatcttg tataacaaat atggctgctg gcatcttaaa tcaaccacta agtcatgagg
     2821 aagtaataga aactactcaa aaagttaaat cagaatttat gaatttagtt aaaacaacgg
     2881 ttaaaaattt ataagttaaa gcttattata gattttttga ggaagtttat aataaaatag
     2941 ttaatgttta atttgaaaag aattaataaa aagctgtctt gatatagaaa gttatttcta
     3001 ataaaggaca gtttttttat aatactacaa catttgaaag tgttttaaag tttaatattt
     3061 atttaaatta ttataggtta gaatggagat ttcaattatg agaatgtatg atattataaa
     3121 aaaaaagaga gataacctag agcttagtaa aaaagagata aactacttta tagaaaacta
     3181 ttcaaaggga gatatacctg actatcaagc ttctgcacta ttaatgtcga tttacttaaa
     3241 taagatgaat aaacaggaaa ctgtacatct tacagaagca atgatgaact ctggagacat
     3301 gataaaccta tctgagatag atggaataaa ggtagataag catagtactg gtggtgttgg
     3361 agataagact accatagctc ttataccttt ggttgcttca tgtggagcac cagttgcaaa
     3421 gatgtcaggt agaggtcttg gtcatactgg tggtactatt gataaattgg agtctatacc
     3481 aggtttttcg acagaaatgg agataagtaa atttattgat tctgtaaata agtctaaaat
     3541 agcagtttgt ggtcaaagtg caaaggtagc aattgcagat aagaaaatat atgctcttag
     3601 agatgttaca gcaactgttg ataatatatc attgatatca tcaagtgtaa tgtctaaaaa
     3661 gttagcttca ggagctgatg caattgtgct agatgtaaaa actggtgatg gagcatttat
     3721 gaagacatta gaagagtctt ttgagttagc taaatctatg gttgatatag gtactggtat
     3781 gaatagagat acaataggga ttgtaacaga tatggatgaa cctttaggtt ttgcagtggg
     3841 aaactcttta gaagttatag aggcaattga gacattaaaa ggaaatggac ctaaagacct
     3901 tgttgagcta tgcgaagtat taggagcata tatgttggtt ttatcaaagg tagcacatga
     3961 ttttgaagat ggtgttgaaa aaataagaga atcaataaaa tctggtactg ctatacaaaa
     4021 actcaaagag tttatagaga atcaaggtgg taataaagat gtagtagatg attattcttt
     4081 attcaaaaaa tcaaagtata aatatccagt taaatcaact aaatcaggtt atataagcaa
     4141 aattaaagct gaagatgttg gtgtatcggc tatgattctt ggagcaggta gagaaactaa
     4201 agatgatatt ttagatttat cagcagggat aattttagaa aagaaagtag gagattatgt
     4261 aaatgaaggt gaagttttag cttacatgta ttataatgat gaacaaaagc tttcattggc
     4321 aaagggaaga tttattgatt cctactctat tgtagattca aaaatagaaa aaaataaatt
     4381 aatatatggt attgtaacta aagaagagat taaaaagttt tagaaaagtg attataaaag
     4441 gcctaacatg tcaaaaataa tgttaggttt tttatttttt gataaactat aaattatacc
     4501 aaaaatatat attttaaaac attttttaca atataaataa gtttattcta caagatgatt
     4561 gaaaattaaa gtatctagtt ttataataga aaatggataa atatacataa ggagtagatt
     4621 atattatgaa ctttaaagaa aatagattga aaatagcagt attaataatt gttattttga
     4681 ttatcttagc aggtatattt gtgtttatac aaataggtcc ttatgataaa aataacaaaa
     4741 aagatgtaat tatcgacgtt ccaagtggag catctgttgg aaaaatatct gacatattat
     4801 atgaaaataa attaataaag aatgaactat tatttaaact tttggtaaag gttagcaata
     4861 aagctccgtc tataaaatca ggaacatact tacttaatca atcgtattca aacaatgata
     4921 taataagtct tttagtttca ggtaaaatat atcaggatgg tataaaagtg actattccag
     4981 agggagctac atctaaagaa attatagcaa tgttagtaag taaaaattta ggagataaag
     5041 ctacttttga aaatttaata aaaaaacctc aagaatttta tgataaattt ccatatttga
     5101 aggaagatgg aataacctct ttagaagggt tcttgtatcc agaaacttat tattttaatt
     5161 caaagaaaca atcagaagaa gatattttaa gcgaaatgtt aaaagtattt gacagtaaat
     5221 acacggacaa gtttaaaaag aaacaaaaag aattaaatat gactcttcaa gaagtaatgg
     5281 aaatggcatc aataattgag aaagaagctg ttcttgacaa agacagacct ataatagcaa
     5341 gtgtgtttta taatagactt aaggtaggta tgcctcttca atcagatgct acaatacaat
     5401 atatatttga agagagaaag aaaattgtaa catatgatga tttaaaaatt gattcaccat
     5461 ataatagtta taaaaataag ggattacctc ctacaccaat atcaaatcct ggtataaaat
     5521 ctattgaagc agcattatat ccagagaaaa cagactattt atattttgta gctaagattg
     5581 atggtggaaa taattatagt acaaactatc aagaccattt aaaatacgtt aaagaatata
     5641 aagaagcaag ggataaacaa tcaaaagata caaaagcaac aaataaagaa aatacaaaaa
     5701 aataaaccat gattataaaa agtctaacag ggtaagaata atgttgggct tttttattga
     5761 aaattgttat tcttagattt ataataaa
//
LOCUS       DAENCV010000189         5724 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAENCV010000189 DAENCV010000000
VERSION     DAENCV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.192"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..585
                     /locus_tag="KQD82_003581"
     CDS             <1..585
                     /locus_tag="KQD82_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382019.1"
                     /translation="IINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDLMNQLT
                     QANNTITQLNTKYKVASGTVTPFKGDDTKIAYPYLTDRVFKPSAWVKISNLDFKPNIF
                     FADFDYYDTEYKNNYKLFLFACCGVATQRGVDFSSVTSFVRKSSDEYFHANGWLYNNS
                     EGDVYFNNTGVQIPAYSFDSTQRHIYKWYAIKFI"
     gene            600..896
                     /locus_tag="KQD82_003582"
     CDS             600..896
                     /locus_tag="KQD82_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382020.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELHYIDIPYGS
                     IDYIKNRIVGINIETKQPILEEIPVYVSEAEKEKQELENQLLLLTNQEIGGGIL"
     gene            896..1078
                     /locus_tag="KQD82_003583"
     CDS             896..1078
                     /locus_tag="KQD82_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382021.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNRPFELDDVTNTEYRKAVED
                     YIIEHSGVVEGAEPAK"
     gene            1113..1343
                     /locus_tag="KQD82_003584"
     CDS             1113..1343
                     /locus_tag="KQD82_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG4382022.1"
                     /translation="MNEEVIKEKIKRNEIRINRHSDEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            1363..1620
                     /locus_tag="KQD82_003585"
     CDS             1363..1620
                     /locus_tag="KQD82_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG4382023.1"
                     /translation="MDSLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            1620..2417
                     /locus_tag="KQD82_003586"
     CDS             1620..2417
                     /locus_tag="KQD82_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG4382024.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCEIANRQNVDVAVQIHFNADHTTLNSMGTETIYKTNNGKIY
                     ADRVNTKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKILANVLSWGYSPSKVLVCDIKDYV
                     PGQTENLYVVGGGACEKISSITKEKFTMIKGNDRFDTLYKALDFINR"
     gene            2498..2860
                     /locus_tag="KQD82_003587"
     CDS             2498..2860
                     /locus_tag="KQD82_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382025.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            3026..3544
                     /locus_tag="KQD82_003588"
     CDS             3026..3544
                     /locus_tag="KQD82_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382026.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(3608..5194)
                     /locus_tag="KQD82_003589"
     CDS             complement(3608..5194)
                     /locus_tag="KQD82_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382027.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(5170..5691)
                     /locus_tag="KQD82_003590"
     CDS             complement(5170..5691)
                     /locus_tag="KQD82_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG4382028.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
BASE COUNT         2100 a          670 c          900 g         2054 t
ORIGIN      
        1 attataaata ctttagcatt aaagaatata gaggcaagtt taaataatac attagtcgag
       61 ttatcagaga aaataaaaac aagttttgat agttctgacg ctagtgtgca ggatttgatg
      121 aatcaattaa cacaagctaa taataccata acacagttaa atacaaaata taaagtcgca
      181 agtggcacag ttactccttt taagggtgat gacactaaaa ttgcttatcc atatctaact
      241 gatagagttt ttaaacctag cgcttgggtt aaaattagta acttagattt taagcctaat
      301 attttctttg ctgattttga ttactatgat actgaatata aaaataatta taaacttttc
      361 cttttcgctt gttgtggtgt tgctacacaa agaggtgttg atttttcaag tgttacatct
      421 tttgttagaa aaagtagtga tgagtatttt catgctaatg gctggctcta taataattcc
      481 gaaggagatg tttattttaa taatactggt gttcaaattc cagcttatag ctttgactca
      541 actcaaagac atatttataa atggtatgct attaaattta tttaagaagg agaagaaaca
      601 tgaatgttcc aaatagagta atatatgacc aaacaggtaa aataattttt gaaacaggtg
      661 agtcctgtgg ggatgtgtta ccacacaata aaataactga attacattat attgatattc
      721 catatggaag tatagattat attaaaaata gaattgtagg tataaatata gaaacaaaac
      781 agccaatttt ggaagaaata ccagtatatg taagtgaagc agaaaaagaa aaacaagagt
      841 tagaaaatca attactttta ttgacaaatc aagaaatcgg aggaggaatt ttataatgaa
      901 tataaataat gttgtggtaa gaatattagc agaaagaata ttaaatggag ggttaaaccc
      961 tttgaaaaac agaccttttg agttagatga tgtaactaac acagagtaca gaaaagcagt
     1021 agaggattat attatagagc atagtggagt ggtagaagga gcagaacctg caaaataggt
     1081 tcttttttaa tatcaaaaat taggaggctt acatgaatga agaagttata aaagaaaaaa
     1141 taaaacgaaa tgaaataaga ataaacagac acagtgatga aatagacgaa ttaaagatag
     1201 cgaatataga gtctaaagca gagttaaaag cattgtgtga gaatctaaac tcacttacaa
     1261 gtatgttgaa gtggctaatt ggtacaatga ttacaacact tgtaggattc tttatatttg
     1321 cagttcaaag aggaatattt taattaatta ggaggttaag gtatggatag tttaataagt
     1381 tttataccag agcaactgct tttattagta gttgctctta atgtattagg atttggattt
     1441 aagaaataca aacagttaga taataaatac attccaatca tattgcttgt gataggaatt
     1501 ggattttcaa tatggatgtt aggatttaat ccttcatcaa ttttacaagg aattttatgt
     1561 tggggagttg caataggggc aaatcaagtt tacaaacagt tgaaggatgg tgataaataa
     1621 tgaaaatagc aatagtacca ggacacactt taagtggaaa aggaacagga gcaactgggt
     1681 atatagatga aggaaaagaa aacagaatcc taactgattt aatagtaaaa tggttgaaac
     1741 aaggtggagc tactgtatat actggaaaag tagataagtc taataactat ttagcagaac
     1801 aatgtgaaat tgccaatagg caaaatgtag atgtagctgt acaaatacat tttaacgctg
     1861 accatacaac attaaattct atgggtacag agacaattta caaaactaat aacgggaaaa
     1921 tatacgcaga tagggtaaat actaaattag caactgtatt taaaaacaga ggtgcaaaat
     1981 cagatgtcag agggctttac tggcttagtc atacaaaagc tccagcgata ttaatagaag
     2041 tgtgcttcgt agatagcaaa gcagatacag attattatat tagacataaa gacatagttg
     2101 ctaagttaat agcagaaggt attttaaata aaaatataaa taatgaggga gttaaacaga
     2161 tgtacaaaca tacaattgtt tatgatggag aagttgataa gatacttgcg aatgtgctta
     2221 gttggggcta tagtccaagc aaagttttag tttgtgatat aaaagattac gtaccaggtc
     2281 agacggaaaa tttatatgtt gtaggaggtg gcgcatgtga aaagataagt tctattacta
     2341 aagaaaaatt tactatgata aaaggtaatg atagatttga tacactttat aaagcattgg
     2401 attttattaa tagatagata aaagttatca actagaagtg gttgtttgtt gtgataactc
     2461 tattattgta atataatgta aatatattat tgtgataatg gaggatttat gtatggatgc
     2521 attggtttat gagagatttg taagagcagc ttttagtatt aaacttaata acttgattaa
     2581 tagaagcgaa gatttaggtg gattggctga agctgatatc tttagagcag caaataattt
     2641 acatgaatta aatgagataa aaataggtac tgggtatgct attgcaatat ttaataatga
     2701 gatattagag tcttgtaatg tttctgataa tgatagcaat agaatgattg aactatttga
     2761 tagaagttta attgcaactt ctagggagga aatattggat attataagag aatatgaaac
     2821 ttatagagga cgatatctta cttttaattg gaagagatag aatgtttaag ttaggaagta
     2881 gtaattatag ttagttatta ctttctttca catattttta ttatttcaat aagattattt
     2941 ccaaataata atttattcaa tgataataag tagaaaataa aattttgaat caatataaaa
     3001 tttaatgaaa tgaggtagta aaatcatgga taaaaaaata gacatagcga agtttatagt
     3061 aaatagaatt gattactata ttgaaaaatc agataataaa gctagttttt tactattatt
     3121 aaatagtgca attataggtt ttttattttc agggaaggaa aaaataatga atcacttaag
     3181 tataagtaat atcagatgtt tagagatgct attttacatt gttatattat gtatatttgt
     3241 tatttcaatt tatttttcga ttatggtttt aaagccaagg aattctaaaa tagaaaatga
     3301 atataaatct attttttatt ataaagagat agcatcttta aataatgaac agtacaaaga
     3361 agcatttgaa aatgcattta aagacgaaga aaacttgatt aatgatgcac ttgttcaaat
     3421 aaaagaatta tctttaatct gtgataagaa aatgtataat gtaaaaaagt cagtagaagc
     3481 ttttatttta ggtggtatta tattgtttat atatatactt gtagtagttt ttaatgctat
     3541 ttagaaaatg ttttatttgg ttagtattcc tctatgaaga tagaatcaaa tcatagaaga
     3601 atactattta catatttgta gattcttgta attgattttc atttaatttt ttaaatgcta
     3661 gttttaaagt ttctttaaaa tcaggtactg tgttttcatt aaaatcttca agttcatcct
     3721 ctcctatcca tgcaagttca ttatgttttg atgtgggttc aaatttagaa atatcataat
     3781 catttattat ttctgcgaga gttataatgc ctttgtgtaa accatcactt tttttaactt
     3841 gataaattgc tagaggtata ggttggcaat catcttttct tgtgcaatca gtaataggtt
     3901 caatgtttat attaaaatct ttttcatatt catctttaat agtatttata attgaagttt
     3961 ctagacttgc cttagcacaa ccaaattccc atttactcgc attattattt ctattatcac
     4021 ttcttttcgc aattaatatt tttgaagttg atttattata acaaaccgca acacagtgta
     4081 attccattaa atctactttt atatagtctt tatctattgt atttttatca taactggggt
     4141 ttgttttaga aattacgtca cctatttttt caatcttatc actaaggttt ctgtctataa
     4201 gtattttatc caaagccttg tctacagaat taaacataaa agaatcaatt agtaatttac
     4261 tttctccttt tttattaaaa aaatattctc gaaccaattc attttcttct atttcatcaa
     4321 aagaaaaact atcatcaaat tctatatcat tattttttcc tttattatga taccaaatga
     4381 tagggtatag tttatctttc cagataccct ttaatttttt atatgtaatt atatgtaatt
     4441 tagataaaat atcagttttt cttgataata tataagctag ttcaaaacta agaacaagtc
     4501 tttctgggca tgtttgcttt gatattctga aaccagcatc aatatcattt cctaagaact
     4561 caaatatttt ataattattg gataaatcgt acatagcaga aatattttca tattgttcat
     4621 tattttctaa agcattaaaa ttaggatttc ttgatactat agcaatccag gccgctcctt
     4681 ttaatgaaat aatattttga agtttcatta agtatttttc agattcagaa aatccttcta
     4741 gtgtagagaa tatattacca ctttttatat ctttagcagt gcttgttaaa atatcaaaga
     4801 ttatatctat gtatttataa atttcatcat aattttttag tacgataatg aaaattacct
     4861 catcaccaag aactctccaa agttgtgctt taaggttttc ataaactcta tattgtaact
     4921 tacgaattat atgtgataat accttagccc atccgctata atttatattt ttatataatg
     4981 atgaattagc tatatcaaaa gaaaagaaca aaatcacttc tggatgatta ggttcttttt
     5041 ctttttcata aatatcagta ttaatatttt cttgaagttt ttccttatac tttttaagat
     5101 taaaagcgtt ttcgttttta gctgaattat gaacattatt tacattttga gttttatatt
     5161 ctttatcatc taccattgta aatatcctaa ccatttccct ctattagatg ctgcataaga
     5221 ggaaacatta aagtattctg caatttttga agtgtcgacc ttattaccta ctgtgttttc
     5281 atccataatt cttttatatt catgtttagg catcaacaag gctgctgcaa attcattgga
     5341 ttgatactcc ttattggtat tgccacttct aaaatactga tttccatctt gactattcca
     5401 tagttcatca tcaatgccat aacccatgtg taaaaataaa tgtccaagtt catgagcaat
     5461 ggtaaaattc tttctggtat ctggttgata aggagatacc actatttcaa atgaatcatc
     5521 aacttttcta ataaatccat cagagtatcc acttaaagaa ctatcttcta ttaccttgcc
     5581 tcctaaagca tcaacaactt gatttatatt tactattgga atttggattt tgtatgtttg
     5641 gataatatct tgggttatat cattaattag ctctctgatt ttgaaatcca taaaatcaca
     5701 tccttatata atattttatt taaa
//
LOCUS       DAENCV010000190         5720 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAENCV010000190 DAENCV010000000
VERSION     DAENCV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.193"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(839..2245)
                     /locus_tag="KQD82_003591"
     CDS             complement(839..2245)
                     /locus_tag="KQD82_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG4382029.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(2290..2997)
                     /locus_tag="KQD82_003592"
     CDS             complement(2290..2997)
                     /locus_tag="KQD82_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4382030.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     KVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            3168..4082
                     /locus_tag="KQD82_003593"
     CDS             3168..4082
                     /locus_tag="KQD82_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382031.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            4082..4843
                     /locus_tag="KQD82_003594"
     CDS             4082..4843
                     /locus_tag="KQD82_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382032.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4855..5592
                     /locus_tag="KQD82_003595"
     CDS             4855..5592
                     /locus_tag="KQD82_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382033.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1973 a          885 c          712 g         2150 t
ORIGIN      
        1 taaacttccc acttactaaa tttcttactt actactactt ataactaaac ttcccacttc
       61 ctaaatttct tacttactac cacttatcac taaacttcct actttctaaa tttcttgctt
      121 cttaagaata atgttaattt taattttaag attatagttt ttaccttgtt taattgaaac
      181 attctatata attatatcct tctattttag agttttttta tttatatatt acaaagttta
      241 aattttttca ttaattatat tataaatttt ccatatattt caacataaat acatatatat
      301 atatgttatt tctaaaaata ctacacaaaa actctacttt agtagaactt aatggcgcaa
      361 aatacttcaa tttaaaacat ttatcgtact ttaaataaat aactataata atatacttac
      421 aatagttaca tagaagctat tataactaca cagaacagcg tataattaaa acttttttag
      481 gatgtcatta tattttttct taaaattaaa aaacactata ccataattct attttagtag
      541 aatttttttg tcaataataa aattaaatat taactaaaaa ataaaaaaaa tggaattatc
      601 taaagaattt aataacctta caataattcc aaaatattat ttctatgaaa attttgatac
      661 catgagtaac tcagccataa gcgcataaag cacaagttac tctttacttt aataaaactt
      721 gacaagaaaa gaatttatcc ttattctact ctaatagagt taatttgtca ataaagaata
      781 aaattatttt caaaatattt tcattcttca ttcttatctg ttattatttt ataaaatatc
      841 acttaattag aggtaatttt agtattgttt ttagtccctg actatcaatg ctcgtaatat
      901 ctaatttacc tctatgtgca ttaataatgc cagatactat caaaatacct agaccatgat
      961 tttgaccttt ggcatatgcc tcacttagtc cattttggct ctttaataaa ctattaattc
     1021 tttcttgact catacctttt ccatcatctt caacaattat atagcaaaaa tctaaatcag
     1081 acttagaggt aacccttata ttacatcctt gttggttatg agttatacta ttttgtatta
     1141 aattactaat agcccttttt aataattttt tatctgcatc tataataata tcttcattta
     1201 gtatatcaag ttcaacgtaa aagtcattat ccaaattatt atttataaac tcagaaataa
     1261 gctctctcaa aaacgcagaa actctaatct tatcacaatt caatgcttgc atattatatt
     1321 caagtttaga cactaaattt aggtcattta ctaattctct gattttaatc ccttgattgc
     1381 atataataga cacttgctct tgttctctat ttgaaaggct actgctttct tcaagttctc
     1441 cagcatatcc aattatcatt gaaagtggag ttcttatatc atgagatatt cctgcaatcc
     1501 agttagacct agcttcctct ttcttcctaa gtttttcatc tttcatttgc aactctttag
     1561 atacagtatt aatagatttt gaaatatcac ttagtattcc tttttcttgt aaaagtactg
     1621 gctctccttt tgacataagc tttatgccag taataattgg ttttatagat ttcaccattt
     1681 ttaaacctat gaatagagct attaaaaaag taactattgc attaaccaaa aacataccta
     1741 ttattaacat aggaaattgt tctatttcct taaccgtata agtaaggtta aacttagtgt
     1801 acttgtcttt tggataacct gttactaata ttccatttgg atgttcccat acatacacag
     1861 gatagtcctt taaaaagaac cttgaaaact ttgctacatc tgtcaaagta taatgacttg
     1921 gaatttcttt tggtaaatca aatccccata ttctatctcc attgttatca attaacattg
     1981 cccaaacttt atttttatgt agtatttcct tactagaatc atctaaaata aactttccat
     2041 cttttaaaac caaattatct tcaatcataa tcaagtttga ttttatttct ctgctatcac
     2101 ttgtatctaa attatcagag tatttagata caaataacat atatgaaaac agaagaaaac
     2161 ctgctatatt taatacaata aaaagtaatg aaatcaagat acttgaacct ataaatcgcc
     2221 tcaatatgta ctttacacta tccaaaatta aatcactcct cttgatttct tttattctaa
     2281 tatctaaaat taattaatca tcaacttata tccaagacct cttactgtaa taagatgttt
     2341 aggtgtagat ggagtttttt ctatcttctc tctaaccctt ctaacatgaa ccataagggt
     2401 attttcatat ccatacatat catctcccca aatggcttga catagtgaat cattagtaac
     2461 aattctccct ttattttcat acaactttaa aagcaacgaa tgctctttag ctgttaaaga
     2521 aatctcttgc ccttctttct caacacatgc actatttaaa ttcacaacaa cagaatctcc
     2581 cagtttaaat actggcaact cctcttcttc tataggcaca tatacacgat ttaatatacc
     2641 cacaagcctt aaagtaagtt cctttggtaa aaatggcttt actatataat cgtcagcccc
     2701 taagcccaaa ccaatcaatc tatcttcatc ttcacctctt gcagagagaa aaatcactgg
     2761 tattttagat gtctctctaa tttttctcat aatagaaaaa ccatctccat ctggcaatac
     2821 aacatctaaa attgcacaat ctggctttac atttctaaat accttaacag cttcctcata
     2881 acttgatgct aaaaatactc tactaaatcc ctcttttctc aggatttcat ctatcatttt
     2941 taataattcc ttgtggtcat caaccacaag tattttttta tcttttattg actccataat
     3001 catcaccctc ctttagtata ccatataccc attttcacaa ctttgcctaa atagactttt
     3061 aaggttattg taaggtaaat ttcagctaat tgaaatgtaa ccaagttatt atactgatat
     3121 aagttaaaag aatcaataag cacagattaa aaaaggggat gaaacatatg gaaatgataa
     3181 ttagaacaca aagactttca aaggaataca ataaaatatt tagagtaaag gatatagacc
     3241 taagagttcc acaaggagct gtatatggtt ttttgggtcc taatggagct ggaaaatcta
     3301 ccactcttaa aatgcttctt ggacttgcta aacccacaca aggaaatatt aatattttag
     3361 gtaaagagtt aaatgaaaaa aatagaatat caattttaaa agatataggt tctttgatag
     3421 aatctccatc atactatggt catcttacag ggcttgaaaa catgaccata atgcaaagac
     3481 tcttagattt acctaaaaaa aatgtaaatg aagcattaaa aattgtaagg ttagaaaatc
     3541 aaaaaaataa aaaagttagt caatattctt taggtatgaa gcaacgtctt ggtattgcta
     3601 tggcaataat gaagtttccc aaattattaa tacttgatga acctacaaat ggactcgacc
     3661 cagcagggat tgaggaaatt agagaattaa taaaatctct accagaaaaa tacgggatga
     3721 ctgttctaat ctctagccat cttttaagtg aaattgacca aattgccaca tcaataggaa
     3781 taataaatca tggcgaactg gtttttcaag atagcataaa agaacttcat aatagaggtc
     3841 aaagtcagat tgctatcaaa actaagaatg ttgtatctgc acaaaaacta ttaaatacta
     3901 aaggatatat gtcacttata caagatgggt acttgactat tgaaaattta aaagactcag
     3961 atgtggctaa aataaatggt atgcttgtcc attcaaatat agatgtattt agaatagaag
     4021 aacataaaaa aagcttggaa gatatattct tagaacttac tggtaaggcg gtgactttat
     4081 aatgaaatac cttggtctag aattttataa acttaaaaga aagaagatta ttctgatgac
     4141 ctttttattt gcagctgttg aaataatgtg gtgtatcgta aattcaaata gagctattac
     4201 acgtaatcca gatatgttag gtggatttga atattcctat atgcttatga gttttgcaag
     4261 tttaaatggt ctattctttc ctatcttaat atcaataatc acatcacgta taagcgatat
     4321 agaacataaa ggagatacat ggaagctttt aaaatcttct gtgacatctt taaatagtat
     4381 ttatttatct aaatttttat gttctgctat tttggtatct atacctgtat tgatgcaagt
     4441 gttatctatt gtgttgtttg gacattttag aggggttatt gagtcaattt ctgtatcttt
     4501 acttttaaaa tacacattag gaacaattct tgtaagtatt gctatcattg cactacacct
     4561 atggattgca actgtaattc caaatcaaat gattgcaatt acatttggca tgattggtag
     4621 ttttataggt ttgacatcag gtttgtttta tcaaggtatt cgtagattgt ttatatggtc
     4681 ttattaccta gagttatcac ctttaagtta tgcttatgat aatgttttag gttcatctgt
     4741 ctatagaatt gatatgaatt tttctatcac gattttagtt ttcttaattg gaatgctaat
     4801 ttattatatt ggaagaaatc atttattaaa aaaagaagta taggagggat ttaaatgtta
     4861 aggaagtcag tcttgggaga aattctaaag ttaaaaaatt cttttatatg gtatgtatta
     4921 ttagctttgc cacttataag tgtgttgatt ggttctggta atttttatct aaatcaaggc
     4981 atactcaaaa aagaatggta cagcttatgg actcaagtta gtctttttta tggggaattt
     5041 ttctttccta tcttaattgc aatcttttgt gcatatattt gtaggcttga acatatgaat
     5101 cataattgga ataatgtaat gacactgcca gttaatttaa aaaatatctt tttatctaaa
     5161 cttacagtag tttcaatact tacagctatt acacaagttt tctttgttat cttttatata
     5221 gttgcaggaa atatgtttgg tttttctggt agtgtaccta aagaattgac tggttggatt
     5281 gctaaaggtt ggattatttc tatttcaata gcttctatac aattatacat gtctataaag
     5341 attagaagtt ttgcaactcc tattggtatt agtctttgcc tttctctatt tggtatggga
     5401 ttaatagtag caaaacctgc tgttggtata tttttccctt attctatgtt aggtagtgct
     5461 atgggggtta taaagcaatc tgctttaaat gttaatgaca ctatagttac aatatgtgtt
     5521 gcatttatta ctactatagt attttctgaa attgccaata gaaattttag aaagaatgat
     5581 gttatttctt aatggtaaat gctagatatt tttgggtttg aaatttatat tctttttagt
     5641 tgaaatctat atccatttta tttaaaaata tatatctttt atttgaattt tatatgtttt
     5701 atctactcgc tatataatag
//
LOCUS       DAENCV010000191         5700 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAENCV010000191 DAENCV010000000
VERSION     DAENCV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.194"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            268..414
                     /locus_tag="KQD82_003596"
     CDS             268..414
                     /locus_tag="KQD82_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382034.1"
                     /translation="MTAYIASLKEYYSFIETQIGDFIKKYGEDVVELCLHRVMILLCE
                     CGLA"
     gene            1246..2160
                     /locus_tag="KQD82_003597"
     CDS             1246..2160
                     /locus_tag="KQD82_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG4382035.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2550..3197
                     /locus_tag="KQD82_003598"
     CDS             2550..3197
                     /locus_tag="KQD82_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG4382036.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3364..3933)
                     /locus_tag="KQD82_003599"
     CDS             complement(3364..3933)
                     /locus_tag="KQD82_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG4382037.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3938..4837)
                     /locus_tag="KQD82_003600"
     CDS             complement(3938..4837)
                     /locus_tag="KQD82_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4382038.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4848..4997)
                     /locus_tag="KQD82_003601"
     CDS             complement(4848..4997)
                     /locus_tag="KQD82_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382039.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4990..5574)
                     /locus_tag="KQD82_003602"
     CDS             complement(4990..5574)
                     /locus_tag="KQD82_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG4382040.1"
                     /translation="MKRFFIVVLGCIFVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2091 a          858 c          659 g         2092 t
ORIGIN      
        1 taatttatat taaatatcta ctaactcaaa tattcataat taaactttaa actggataaa
       61 ttttctttta agaatgaaag tttatccagt tttgcgatta taataaccag ttttacatat
      121 ccacttgtaa aatatatcta agcaatatat catactatat agatttatga gtgaaataat
      181 atctgctatt tactgaatat ttttattttt cgttttttag ggggattagt tgtctttaaa
      241 gataagattg atgaatgtgt tcatataatg actgcatata ttgctagttt aaaagaatac
      301 tattcattca tagaaactca aattggtgat tttattaaaa aatatggaga agatgttgta
      361 gagttatgtc tccatagagt gatgattcta ctatgcgaat gtggtttggc ataaaattaa
      421 atgtattaaa atgggtaaga agaatcctat ataatattct tcttacccat tttaatatat
      481 caatttcata gcttatcgtt taaaatattt tacatccatt actagacaac tttacattct
      541 ctttttaatt ctagtatatt taaatcattt tagttgctcc ttatttattc ttattacttg
      601 ttttatttta taaataaaat aattcttctt aataatgttt tttattgata ttcctatcac
      661 acatccaatt atttatcatt gtcttgaaat tgtttgcata cttaatttaa tccaattgaa
      721 acaccccagc atagaatccc ctgtaaaata gaagttggat taaatcctaa catctataca
      781 gaaaatacta ctccaagcac aagcaatata atggaatata tttatcactt aattttttat
      841 actttttaaa tcccatacct aaaacattaa gtgcagctat tagaattaat aattgttgtg
      901 gtataaaatt atgcttgctt catatttatt cattttttgc tataaaagtt cttgttttac
      961 aaaaaacatt tgttttgtat caaaataata cttataaatt acataattct cataaaaatt
     1021 tctagtatta cttgtactct taataaataa tagccaacaa gtaccttaaa tcattatatc
     1081 tatatctaat aagaaacaca gtaagataat tttttcgccc ataacttaat aaaaactgtt
     1141 gatattagta gcaattgaat ttagttaatt tatttataat atacataaat ttaaatatta
     1201 cgaataaagt attataaata ggatactttc taaagggagg aaattatgat agataatcaa
     1261 aaatatgtta ttttatcact agaattacat ttattttttt caagaattat gaaagagcat
     1321 gctctttttt tagaagcagg attcacaaat aaaaattata atcttgctat ggaagctgac
     1381 cactataaaa agcaatttga agatttatta tcatacactg ttagtgctag taatggtata
     1441 attagacctg atatattata ttcagaagag cttgtaacta ctctcacatc agttgcagaa
     1501 caaaaaacag aagagtttac agggatagaa ataaacaaaa acatcactac aagagaatta
     1561 aatctacaaa gtggtgtaaa cccacaagtt ggtcaagact tagtgaacta tgtagctcaa
     1621 cttaactctg atgcaataag attacttgat gggcttatta attttaaaga aagagtctta
     1681 gatggtgtac tatcatgtac tatatttaca tcaaactacc ctctacttct tgaacatata
     1741 atacatgaag caaatttata tcgttcttat gtagttgacc ttgaaaataa aatagatatt
     1801 gagtcaaaaa acgctaaaga aatagaatta ttctgggacc atattatgat ggaacatgct
     1861 ctgtttatga gaggattact agacccctca gaaggtgaac taataaatac ttcaaatgat
     1921 tttgctataa aatttaatga attaattgaa aaaacaaacg aaatgactga ttctaatatc
     1981 aagaacatta cagaagaaac tctaaatgaa actgttgagt ttaaagattt taaagaagca
     2041 ggagcatcag gaatagaaca gtgtaagata aaatctataa tattaccact tttagcagac
     2101 catgttttaa gagaggcaaa tcattatatt agaatattgg agagttataa aaacatgtaa
     2161 ttaaaaatta cttttaaaat aatctttgca tatttagata aaataaaggt acagttaact
     2221 gtacctttat tttttataaa cttataaaat ttaaacaagt tattcataaa ataaaataat
     2281 aatcatctat aacccacaac tttattttat attaaataaa aagtattagt gattatgttt
     2341 tataggtatt tctttattca gttatttatt aaaaactaat agtaaatctt atactaatcg
     2401 catatttatg ttataactat attgtaacta ttttcgtaat cttaggaaaa ggttacttat
     2461 aaaattaacc ttatacaata atatatcgta taatcttaat tagcataata gttacaatat
     2521 taaaatttaa atatgggagt gattttagta tgataagaca aacaccatta gagaaacgat
     2581 atgacaagct aggtcctcat attgtaacag cactcaaaaa acgttatttt gatgcttatt
     2641 actgtaaaac tagaaatgaa gctttgcaac aaattttaga tttaattcct caaaatcatg
     2701 tagtatcatg gggaggatct gaaacactta aagaaatggg cgtacaaact gctgttagag
     2761 agaaaggatt taaagttatt gatagagatt tagcaaaaac tccagaagaa agaactgaaa
     2821 ttatgcgtca agcacttcta tgcgatactt ttttgatgag tagcaatgca attagtgaag
     2881 atggacaact ctttaatatt gatggaaatg gcaatcgtgt agctgcaatg atatttggtc
     2941 caaaaaatgt tatagttgta gctggtatga ataaaattgt aaaaaacatt gatgatgcaa
     3001 tacaaagaac tagaacaatt gcatctccag cagttgttca gcgttttcaa gatgtcaata
     3061 caccatgcta tattaatgga agttgtatag attgcacaag tcctgaaagc atttgtgcat
     3121 acatggttac tacaagaatc agtcgtccag caaaaaaaat aaaggtaata ttggtaggag
     3181 aaaatcttgg tctataaaat atacatacat gtaaatagct ctataaataa taaaatcata
     3241 tgaaataact tatttctaaa agtggtagaa taaaagcttc tacaagactt tcattctacc
     3301 acttttatat tcttttaatt tgtgtttcta tattattaat tgctccgatt ttcatatata
     3361 ttactaacta tttttatttt cataattttt ttgttcttca tcaagctgtt tataataatc
     3421 tttattatag tcagctagat aatctatacc acctcctgta atcacattta ataatttcat
     3481 tgcagattct tgattaactt tacaaacata attatcacca gatttaacaa gctctaatgt
     3541 aatattaaat tcttttttct ttgcagaatc aatatctttc atagttggat ttggcatcat
     3601 ttctcctagt ttaggaggat tcttttttaa aatagaaacc atatcagtat tgttaatttt
     3661 taaatctaca gtcactttat ccttattatt ctttatatct gaaatttccc aagtcatatt
     3721 tttagaatac attttaaatc cttctttatc ttcgtatata agattaccta accctgattt
     3781 tgggaaatac ttatcaacag tatctgcatc ttgtgttttt attgaattaa acatactatc
     3841 cacttcattt tttatcttat tatcattagt acaaccagct aacaccagta ctgatattgc
     3901 aaacattata atacatataa taaatttctt cattaaatta tatcccccta tacttttttg
     3961 cacaaatatt attctctttt ttatcttttg aaaaagttgt agtaattgca ttttgtggac
     4021 aaacttttat acattcacca cacctaatac agtctggact atttacatct ttatatattg
     4081 gtatatcaag cttacatttt gtttgacatg ctgtacaatt tgtacattta tctttatcaa
     4141 ttttaaatct atatattgaa ataggattaa aaaaaccata gattgctcct aaagggcata
     4201 tatacttgca aaatggccta taagtaatga ttgacatcaa taatatcaat agcaaaatac
     4261 ttaacttcca gctaaataaa aatcctattg attctcttaa attctcattt cctaaaataa
     4321 gtggtattcc tcccaaaagt gttcctgaag ggcaaatcca tttacaaaac caaggatttc
     4381 caccccctgc tacatttaca atagcaactg gcaaaataat tacaaacatt attaagacta
     4441 tatactttag atattttaga tatttatcac cataaacttt ttttatcttt actggaaagg
     4501 gtactttatg tagtaaatct tgtacaagtc caaagggaca aagccaacca caaacaaatc
     4561 tgccaaaaaa agcaccaaat gccattaaaa aaccaacaac ataaaatgag aatttatact
     4621 ctctgcttga taaaactgct tgtaatgaac ctattggaca tgctccaagt gcacctggac
     4681 atgaataaca attcaatcca ggtacacata attttttact ttctcctcca tatatagttc
     4741 cttctaaaaa tccatttaca tatccatttg ttaatgctgt aaatgcaact tgagtaataa
     4801 gtcttatatt attatttact ttagataata acttcattcc tcttctccta accaatacca
     4861 atacactcaa gacaaatcat aattgcttta gttaatactg tatttgtttc tccacgatat
     4921 gtacctaatg caataaatat aatactaatt gcaataatag aaatgccttt tttattatct
     4981 ttaataaaat tactcattgg cttttaccat ttttagtctt tcatctataa tttttttcca
     5041 atcttcctta ctatttgcac ctacaacctt ctcacccaca aagtttcctt tcttgtctac
     5101 aaaataagtt gttggaaact ccataactct tttaaaatca gtttttttaa gttcttcaga
     5161 tacagtcaat tgttggtaag ttgcatctga ctttttcata atgtctttaa ctaaattttt
     5221 ctcttcattt gaaagaccag ttctcatatc agttttatca acttctacaa ctaatccctt
     5281 tatagctaca ttggaattat tactctcata ttcttttgat aatgcttcaa gctctggcat
     5341 ttcacccaca catggtccac accaagtagt ccagacatta ataagagtaa gttctttatt
     5401 tgaaaatatc tcttttgtta ccttatttcc atcaacatcc tccgtatcta aattactaaa
     5461 ccattcacta ttattcttgt ttttttctac tggcttagat tcttttttgc tacatccaga
     5521 aatcatacct aaaacaaaaa tacatcctaa aactactatg aaaaaccttt tcatatctca
     5581 cactcctaga atttatatag tatataaaat cgctgcttga aataaaaata acttatcttc
     5641 tatattaaag taattatatt taataaataa gagctaattt aaaaaaagat atacagttaa
//
LOCUS       DAENCV010000192         5581 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.195, whole genome shotgun sequence.
ACCESSION   DAENCV010000192 DAENCV010000000
VERSION     DAENCV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.195"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            86..817
                     /locus_tag="KQD82_003603"
     CDS             86..817
                     /locus_tag="KQD82_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG4382041.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            897..1532
                     /locus_tag="KQD82_003604"
     CDS             897..1532
                     /locus_tag="KQD82_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG4382042.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1783..2376)
                     /locus_tag="KQD82_003605"
     CDS             complement(1783..2376)
                     /locus_tag="KQD82_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG4382043.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2447..3310)
                     /locus_tag="KQD82_003606"
     CDS             complement(2447..3310)
                     /locus_tag="KQD82_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG4382044.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3473..4642
                     /locus_tag="KQD82_003607"
     CDS             3473..4642
                     /locus_tag="KQD82_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG4382045.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            4743..5219
                     /locus_tag="KQD82_003608"
     CDS             4743..5219
                     /locus_tag="KQD82_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG4382046.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         2002 a          814 c          690 g         2075 t
ORIGIN      
        1 tataacaata ccttttggaa tatgatatat tatattaatt aaatttttat aagataagtt
       61 atacttatag gagggaacca tataaatgaa tatttatgct catagggggt ttagtggaaa
      121 gtatccagaa aatactattc tagcttttaa aaaatgtttg gatatggata tatatggaat
      181 agaacttgat gtccacagaa ctaaggatgg aaaaattgta gttattcatg atgaaaaggt
      241 tgatagaact tttaatggac atggttttgt aaaagacttt actttaagaa aattaaaaac
      301 tctaaactca tcttttgaag gatatcaaag caataaagaa tgtaaaattc caactttaga
      361 agaagtatta attctaattt cacctacaga ccttatttta aatatagaac taaaaactga
      421 caaaataaac tatcccaata tagaaaaaga cgttttagag cttattttaa agtataatat
      481 gaaaaataga gttcttatat ctagtttcaa cagcaatagc ttaaaaaact ttcataaatt
      541 agaccctagt ataaaaacag gattattgtg ttatttacct ataaacaatg tagtcaattt
      601 tgctaaattt ctgggtaata gttaccttca tccacctcta gttttagtaa atgaatctct
      661 aattgaactt tgtcacaaaa atctacttgg tgtcaatgta tatactgtaa atgaagagga
      721 tgatatacta cactgtctaa aactcaatgt agatggtatt tttactaact atccagatat
      781 tgcttcaaat cttttacact caaaacaata cagctaatta tacaatagtt tgtactcctt
      841 gacttttata taattaatat gtacaatttt attaaattta tcttggaggt ataactatgg
      901 ataacttatt aaatgaactt gaagctttaa agagtaattt agataatcta ccaatagaaa
      961 aaatagaaaa ttatgatttg agtaaaactg ctttatttat aattgatgtt aataatggct
     1021 ttgcaagaca aggtgcatta tactctccta gagtagagtc tttaataaag ccaattgaaa
     1081 tgtttacaaa aaaaatatca aacaaattaa atagagtaat agcatttact gattctcata
     1141 ctcctaaatc aatagaactt ttgagttatc ctgttcactg tttggaaaat gatgtagaaa
     1201 gtgaattggt tgatgaactt aagtcgatag aaaacctaca aattcttcct aaaaattcta
     1261 ctaatggttt ttttgcatta gaaaatttgg attttgataa tatagataat ataattatag
     1321 ttggcgattg cacagatata tgtatatatc aatttgcaat aactctaaaa tcatatttta
     1381 atcaacataa tattgaaaaa aatatagttg tacctatgaa tttagtagat acttatgata
     1441 ttcctaacgt tcaccctgct gagattttaa acttagtatt tttcaacagt atgattcaaa
     1501 atggagttaa tgtattaaaa gagattcgtt aagcttctaa tactttacat cccatataat
     1561 acaattatca gatatttatt tttaataaaa tttctaatag aaaaacacag ttttagtgag
     1621 aagattacaa tttaaaacac tatctattat tactaatctt cacaatataa aaaatcgcag
     1681 tttagatatt ccttatacaa atttataagg aataataaat tgcgattttt atttttaagt
     1741 agtacaatta attgatacta cttcagtatt taaattcatt ttttaataat catacttatt
     1801 cgtagtttcg cacattacta cctttatttt tttattaaac atattcttat attctaataa
     1861 aaactcattt agttctgaat ccataagatt tatttttcta tcactacaaa gcattatctt
     1921 ttcttggtcg attattacat taatatcatt taagtcattt tttataaatg ttgttataca
     1981 actctttggt tggatttttt ctccatattt ctcataaaat cctgcactct tcttaaatga
     2041 ctctaaatct tctgtagata ctttgctgga agtattatta ctcataaaac ctgctgtaga
     2101 agggtcgatt ccaaagtttc taagttgttt ttctacttct tcagtgtcca taccatatct
     2161 ctccataaat tgcttttgta ttttcataaa tttttcttga gatatgttgt tatctctcat
     2221 ataatcgaat atttttcttg aaaactcagc catagacatc tttccatcca taacagaaaa
     2281 atataagtta tatatgtatt gctcaaattt atctacatca gaatctttaa ctttgttctt
     2341 tagttcgtta aagtctatta ttctatcatc agacattttt catcctccta gctaaatttt
     2401 tacttgtata agattatacc atatttctat tatatttata tacacactat ttttttaatt
     2461 taacgatttt ttctgtagca tctaattcgc acattctgcc caaaggaata gttatcattg
     2521 ggatacaatg tcctgactga agattatata tagaaggttt attgacctta tttgcaaatt
     2581 ctattagtaa ttccatcaaa tcattttctt caatacaatc cttaaaatct ccaaaaatta
     2641 taccattaca ttcctcaagt tttcctgaag aatatagttg tgttaacatt ctatccaatt
     2701 tatattgagg ttctccaatt tcctcaataa acaaaatttt atcttttgca tctatttcat
     2761 attttgttcc cattgtggca attattaagg ataaatttcc tcccattatt attccctctg
     2821 ctttaccttc acatacagta taaatctttt cttcttttgg attttctaaa tataattcat
     2881 ctttaaattc taaagcattt accaatgatt tataggtaaa tttatcccat ttacctacat
     2941 ttgctgccat tattccatga tatggtaaaa gaccacaatt tttatagaaa gctaaattta
     3001 aacctgttat atcagaaaaa cctataaatg gttttggatt ttgctttatt atatcataat
     3061 ttattttatc caatattctt gttgttccat aacctcctct taagcacata attatatcta
     3121 catctttatc taaaaacata tcttctatat cccttgctct aatatcatca ttaccagcta
     3181 aataaccttt ataatttaaa ctacaacttt cagaaaactt aacttcgtaa ccataacttt
     3241 ctaaattaaa ctttatttcc tccaaacttg tatttcttaa tggcccagat ggcgcaatta
     3301 ttcctaacat ttaaacacct cttcttataa aatgatatat tcttctccat actaaatatc
     3361 tctaatataa ttataagata ttttatttgt acttgataca taataatgat ttacataaaa
     3421 taatataatt aatgaagaaa aataaatttt taatatagtt aggatgtgtt ttatgcttaa
     3481 tataaaaact ctttgtcatg agataaatga ttgggttata aatataagaa gagatttaca
     3541 taagactcca gaacttggat tagaagaatt tcaaactaag aaaaaaatta ttaaatactt
     3601 aaatgaaata ggtataaatt atattgaata taagaatcat acagggataa ctgcatacat
     3661 taacgtgagt ccaaactttg aaacagttgc tataagagct gatatagatg ccttacctat
     3721 aacagaagaa cttaattatt cttataaatc tattaatatt ggcaaaatgc atgcatgtgg
     3781 tcatgatgca cacactgcta ttttactggg tacttgtaat atactattta aattaaagga
     3841 ttacttaaat gtaaatgtaa aattcttttt tcaacctgcc gaggaaacta ttggtggggc
     3901 tcaacttatg atagaagatg gatgtcttga aaacccaaat gtaaaatata tatttggact
     3961 tcatgtaaat cccaatatca acaaaaatct aattgaatta aaatacaaca ctctaaatgc
     4021 ttccaccgat acacttcaat taactgtaca tggttcaaaa tgtcatggag cttatccaca
     4081 tcaaggtatt gatgctattg ttatatctgc tcatattatt acagctctac agacaatcgt
     4141 tagtcgaaac acaaatccaa ctgattctgt ggttatttct ctaggaaaga ttgaaggtgg
     4201 aatcaaagaa aatatagttt gtgacaaagt ggttataagg ggtactttga gaaccttgac
     4261 tcctgaaact agagagtttt caaaaaaacg aattagagaa atttgcgatt ttacttgtaa
     4321 aacatttgga ggaagtattt ctgttgaaat tgaggaaggt tatccagctc ttatcaattc
     4381 aaaccatcta gtagattacg tcaaacaaaa tgctgtagaa ttgtttggtg aagaaaatat
     4441 tatccttaaa gactcgccca ctcttggggc tgaagacttt tcgtattttc taagacactg
     4501 tgaaggagct ttttaccacc ttggttgtgc aaatcgtgaa aaaaacatta cttccccttt
     4561 gcatacctct acttttgata ttgacgaaga ttgccttatt actggtgtca tgctccatgt
     4621 aaaaaatgta ttatcattct aactattaat atttttattt attattttat attgaccctc
     4681 acatgatgtc atgttatacc ctaaaattac aaatgataaa ttaaatagtt ggaggtaata
     4741 ttatgaaaga aatattaata aatcctatag gtaaaatatg tattcaaggc gaagaggtct
     4801 ttattaaatt agacaaaaag tatgtacctg cattaaatga actcgacgat ttcagtcatt
     4861 taaatgtatt ttggtgggct gatgggtttg ataatcctga actaagaagt attttagaaa
     4921 ccccaaaacc ttataaaagt ggaccagacg ttataggaat atttgcaaca aggtcaccta
     4981 taagacccaa tccaatagca ttgactgctg tacaaattat caatatcgac catgaaaatg
     5041 gcataataaa aataccatat attgatgcta atgataatag tcctgttatt gacttaaagc
     5101 catatactcc aagcgttgat agggttgaaa atccgtccgt tcctaaatgg tgtagtcact
     5161 ggcctaagag tgtagaaaaa tcaggagaat ttaattggga agatgaaatt gaattttaaa
     5221 ttactttttt cttgatttca ttaacttgtt ttccacattt attttattta cacttaccaa
     5281 gattttatgt cacatataca tataatatta ggctgtatta tataatacaa cctaatatta
     5341 tataataaat ttctattttt cccttaaatc attcacattc tttctaagct acataagatt
     5401 tttctattat ttctttttga gtttaattta gcattatgtt gaggaaaaaa catttcatgc
     5461 attttaataa attaaatttt tcttttattc tgcgatttta taataatcaa tttaacttaa
     5521 aaataatacc taactatttt aactatgtta aactagtaaa tgtaaccact tatatgttac
     5581 a
//
LOCUS       DAENCV010000193         5484 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.196, whole genome shotgun sequence.
ACCESSION   DAENCV010000193 DAENCV010000000
VERSION     DAENCV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.196"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            297..1133
                     /locus_tag="KQD82_003609"
     CDS             297..1133
                     /locus_tag="KQD82_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4382047.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1486..3063
                     /locus_tag="KQD82_003610"
     CDS             1486..3063
                     /locus_tag="KQD82_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4382048.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRIAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3056..4240
                     /locus_tag="KQD82_003611"
     CDS             3056..4240
                     /locus_tag="KQD82_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4382049.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPIGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4258..4857
                     /locus_tag="KQD82_003612"
     CDS             4258..4857
                     /locus_tag="KQD82_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG4382050.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4881..5351
                     /locus_tag="KQD82_003613"
     CDS             4881..5351
                     /locus_tag="KQD82_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4382051.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2066 a          568 c         1041 g         1809 t
ORIGIN      
        1 taattaagtt gaaatacttg aaaatactgg aaaaaattga aaataatttt tgtaatataa
       61 aatgtcgatt gattttaaat aattaaatat acataagaaa taattaaata tctttcaatg
      121 aaaatatgat aaaatatgac aaattaattg atgacaataa aaaatatttt gttattgtag
      181 tattataggg taaattgcaa aatatatacg aaagtaaaat tttaaatatt gacattaaat
      241 taaatatact atactatgta agtgtgatga gagaattttt ttagagggga tttatagtga
      301 atataaaaaa gatatttaat aacaatgtag tagtaagctc cctagaagat ggcacagaaa
      361 taattgtcac tggtgctgga gtaggattta agaaaaaagt aggcgatttg atagatgaaa
      421 atttgatttc taagaaatat tttgttcaag acgatcaaag ggacaaatac aatcaaatac
      481 ttaataagac ctctattgaa tactttaaaa tttcagaaga aattatagaa aaggctaatg
      541 aagttttaaa tactcaagtg aatgattcaa taatattagc cttaactagt cacatagaat
      601 ttgctgtcca aagagaaaaa caaggtataa aattaccaaa cttaatattg aatgagacaa
      661 aacaacttta tagggaagaa tttgaatttg gattatgggc aattgatgaa attgaaaaaa
      721 ggataggaat aaaattgcct gaagatgaag caggatatat tgctattcac ataatcaatg
      781 gtttggaaaa ttctcaaaaa gatgaaggta ttaatatact agagttttca aaacaagtta
      841 ttcagataat tgaagaagta catggattta aattggatgt aaattcatta aattatacta
      901 gacttataac tcacttaaag ttttttgttc aaaggattct ccgaaaagaa acttacaatg
      961 atagtgatat tgaagatatg tataagttag tgaataaaaa ttacaataaa cctaaagtgt
     1021 gtactgaaga aattgcagca ttggtattag acagatttga atataagatt agtaaagaag
     1081 aagaattata tttaatgatt cacataaata aaataacaaa cagtggttgt taggattgta
     1141 actgataaag caggcaaaac ctaagtcatt gagcttaact attagattaa gttttttgat
     1201 ttaggttttt ttattagata caatcaaaat acagacagtg acttttagga ttgtaactgg
     1261 ttaagcaggc aaaacctaaa tcattgaatt taactattag gttaagtttt ttgatttagg
     1321 tttttttatt agatacaatc aaatacagac agtgactttt aggattgtaa ctggttgagc
     1381 aggcaaaacc taaatcatta aacttaatta ttaaattaag ttttttgatt taggtttttc
     1441 tataaatttg attatgtttt tagataaaaa taggagggga aaattatgaa aaaaaataag
     1501 ttaggtttat ggaacttctt ccaaatgtta ggaaaaacgt ttatgttccc aatagcacta
     1561 cttagtgttt ctgggatgat gctaggactt ggggcaggtt ttacagaccc taagatgatt
     1621 gaaatggttc catttttagg gaatgaatat gttaacttag ttttaaactt catgttaaca
     1681 ataggattat ttgcatttaa caacttggga gcattatttg caatggcaat tccactagga
     1741 ttattaaaaa aagaaaaaga gtttggtgct ttttcaggat tagtaggatt tatagcaatg
     1801 catataggta caaattttta tttaacaaga gctgatttgt tagtaccagc agaacagatg
     1861 tcaactaatg gtcaagctat gattatgggt atacaaacat ataatacaag tgttttaggt
     1921 ggtattgtag caggattaat tgtttatgca atatatgata aagttagtaa tttaaagata
     1981 ccagaatcat taggattcta cagtggacca agattagttc caattgttag tttaatagtt
     2041 atgagtatag ttggattatt aatacctatt gtttggccac catttttcaa tgcattccaa
     2101 agtttaggaa gatggatttc ttcagcagga ccattaggat atttttcata tgcagtggca
     2161 gaaagagtta caattccatt tggactaaat catcttgtaa catccgtatt ccgttttaca
     2221 ccaataggtg gaactgctgt tattgatgga gaaacgtact ttggtacatt gaacatgttt
     2281 atggcatatg tagaaaataa tcagattata cctcttgatt tagctggtaa aatggaacaa
     2341 ggtaaattga tgattcaata tggattagct ggtgctgcat tggctatcta ccgtatagca
     2401 aaaccagaaa atagaaaggc acttaaagga cttttaatat caggggtgtt gacagttatt
     2461 ataggtggta taagtgaacc aatagagttt ctattcttat tcatatcacc agcattattt
     2521 gctttccata catttatgaa tggtctagca aacatggtac ttccatactt aggcgtgttg
     2581 atgggattca caggagattt aattgcattt ataagttttg gtgtgttaag aggaacagct
     2641 acaggatggc caatagcagt attagtagca gttttatatt ttgcaatata ctattttgta
     2701 tttaagtggg caattattaa gtttaatata aagaccccag gacgtgagga taaagaaata
     2761 aaagtatctg aaggaaatgg ttcagatttc aaaaatttgt ctacatataa aggacaacaa
     2821 atgatatctg ctttaggtgg tcaatctaat atattatcac tagataattg tgtaactaga
     2881 ttgcgtttaa aactacaaga tgtatcatta ataaatgagg atgcaattaa agaggctggt
     2941 ggtatagcag ttgttaaatt ggatgagcat acaatacaag taattatagg aactcaagtg
     3001 tattctttaa gaaagcagat ggataaagct atggaaggtc atttggaggc atgtaatgat
     3061 taattttgat aaaaaagtag accgattggg tacatattgt acccagtggg actatgtaga
     3121 agatagattt ggtaagaagg atttgcttcc ttttacaatt tcagatatgg atttagagtt
     3181 tccatttgaa ataacagagg tgttgcagga aaggttaaat catagagttt tgggatacag
     3241 tagatggaaa catgatgatt ttagaaattc agttacgaat tggtatatta aaagatttaa
     3301 ttgtagcgtt gatagtaact ggatatatta tagtccaagt gtaatgtatt caattagcaa
     3361 acttattgag atgttaagta atgaaggaga tggaatcctt ataaatactc ctgcatacaa
     3421 tgctttttat gatgtgataa caaataacaa tcgtaatatt ataaagtcac atcttgtaaa
     3481 taggaatgga ttctattcaa ttgattttaa tgattttgag aaaaagtgta aaaaatctaa
     3541 gatatttatt ttatgtaatc ctcacaatcc aattggtaga gtatggagtg agttagaact
     3601 tgttaagatg ataggaattt gtaagaataa tgatgtcaag ataatatcag atgatataca
     3661 catggatatt gtatatgaca atagaatgac acctatctta aatgttgcaa aggactattt
     3721 agattctgta ttcatttgta catcagcgtc taaatctttc aatataccag cattgactgg
     3781 gtcatatgtg attataccaa atgaaggtat tagaaataaa tttgaaaata tcactagata
     3841 ccgtgatttt gtaaattcgc cagcaattct agcaatatta gctacaatgg tctgttatga
     3901 tgactgcgaa tattggttag atgaactatt aaaatacctt aaagagaatc ttaaatatac
     3961 gattgattat ataaaagata atttagaacc attgaaattg gtcatgccag aaggctgtta
     4021 ctttgcttgg atagattttt ccgaacttaa aataagtggt gagaaattcc aaaaattatt
     4081 gatggatatt ggagaagttg ctattatgtc ggggtctgta tatggtgaag aaggtgagtt
     4141 ttatttaagg ttaaatgtgg catgttcaag agaaaaactt gaagatggtt taaataggat
     4201 taaaaaaact atggattata ttgcaaaaag aattttatag taggaaggga gatagttatg
     4261 ggatatttaa aaacaatggt tgatgttatg actactgaaa tgaatgccat aagatgttta
     4321 atagatgaag caggaataga atatgaaagt atagttagtg aaattgcaaa ttgtgaaggc
     4381 aaagtaattt ttatgggtgt aggaaaatct gcacatatag gtaagaaatt ggcagctaca
     4441 tttgctagta ctggtacacc tagctttttt gtacatgcaa cagaggcagt gcatggagat
     4501 ttaggaatga ttgaaagtaa ggatattaca attttaatat caaatagtgg aaattctatg
     4561 gaagtggtaa actgtataaa atatattaaa gcaataggtt ctaagacaat tgcatttact
     4621 tcaaacagaa attcagtact ggcaaaagag tgtgattatg ctttaatata tcctgcaaag
     4681 gatgaagcag accatttaaa tctagcacca acaacatcat ctacaattac tttggtttta
     4741 ggagattcta ttgcatgtgc attatctaag agtagtaatt ttggttcttc agatttttat
     4801 aagtaccatc caggaggaag tttaggtgag aagttaaaaa cagctaacaa tggataaaag
     4861 caaaaaggag agtagttttt atgttcaaaa tgtttaaaaa aaaatcaata aaatcaccca
     4921 tatcaggaaa agtaatagag ttatctaaaa tacaagatgg tgtattttct caaaaaatta
     4981 tgggagaggg tgtagctata gattctacag gagacattgt atatgctcca atcaatggaa
     5041 agatagtagt agtagcagag actaaacatg catttgtaat agaattagaa aatgggatgg
     5101 aacttcttat tcatgttggt ttagatactg taaatttaaa aggcgaaggt tttgaggtat
     5161 tggtgagtat gggagaacaa gtgaaggaag gaacaccaat gttgaaaatt aaccgtcctt
     5221 tgattgaatc aaagggtata tcacttatta ctccagttac aataactaat cattcagagt
     5281 acaatatgaa tacatgtaat gtaggcaata gtgttgaagg tgggaaagat actgtaattg
     5341 agtttaaata aagaactaaa atacaaggtt aatatttata aatacctata aacttatgtt
     5401 ttataagatt gattgtatag atttgttgta taaaaaagaa ctctttaatt ataaattaag
     5461 gagttctttt ttattttgta aata
//
LOCUS       DAENCV010000194         5445 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAENCV010000194 DAENCV010000000
VERSION     DAENCV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.197"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(204..1388)
                     /locus_tag="KQD82_003614"
     CDS             complement(204..1388)
                     /locus_tag="KQD82_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG4382052.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KQD82_003615"
     CDS             1761..4112
                     /locus_tag="KQD82_003615"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG4382053.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDNLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KQD82_003616"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KQD82_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG4382054.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2136 a          613 c          866 g         1830 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttactatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgctg
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgataattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcac aaagaacttt gaaaattaaa cagtaggtta atttataaaa caagaaacaa
     5401 accataaagc cagatatttt gataacaata gtatctgagc ctgat
//
LOCUS       DAENCV010000195         5393 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAENCV010000195 DAENCV010000000
VERSION     DAENCV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.198"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(270..2549)
                     /locus_tag="KQD82_003617"
     CDS             complement(270..2549)
                     /locus_tag="KQD82_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG4382055.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEVEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2820..4274)
                     /locus_tag="KQD82_003618"
     CDS             complement(2820..4274)
                     /locus_tag="KQD82_003618"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG4382056.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4438..5307
                     /locus_tag="KQD82_003619"
     CDS             4438..5307
                     /locus_tag="KQD82_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4382057.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1852 a          837 c          564 g         2140 t
ORIGIN      
        1 tttatttttt aaaatatgta taacacacct atagaaatta ctattattac tatctaaagt
       61 attttgctat aatcccatat actcttacct caaaaacatt caaatagaaa aaatactaca
      121 taattaataa actttgccca ataattccta aaataatatc ataacaatga tttatgtttt
      181 ataatagtat atttttacat aaaaaagtga acattgatta acaaagttca cttttttata
      241 aatttttatg ttataattca caattttatt taatttgttt cttttctatt taaactatat
      301 ctatctaata ttaatttttc atattcttct attggcattg gtctagcaaa taaaaatcct
      361 tgagccatat cacaacctat aaaccttaaa aactctgctt gctctacagt ttcaacacct
      421 tctgaaacaa cttttatatc aagttgcttt gccatttgaa caatacttga aactacaatt
      481 tgactctttt ttgcatttcc cactgttata aagaattgcc tatctaattt caatacatca
      541 attggcatat ctttaagcat atttaaagaa gaataaccag aaccaaaatc atccatagaa
      601 attttaaatc caacagattt caaagtttgc attattttaa ataatatttt tgtattgtca
      661 aatattgcac tttctgtcaa ttcaatctca attaaatttg gttcaacttc atattctttt
      721 gttattcttt caagctcaga aataaagcct ggattctcaa gatgtactct tgataagttt
      781 attgatatag gaaatgtggg gaaattttca ttttcccaac gtttaaaatt tttacaaact
      841 tgggtaaata caaacatatc caattctgtt ataaaaccat ttttctcaaa aataggaata
      901 aatacacctg gacttattag acctttttta ggatgttgcc aacgtacaag tgcttcagca
      961 cctacaattt tagtagttga taaatcaacc ttaggctgta aataaacttt aaattgttca
     1021 ttttttaaag catcatgcat ttcttgttca atatcacgtt cttctatgag agtttctttt
     1081 aaattttcag cataaatctc ataaactttt aaatattttc tttttactgt agtttttgca
     1141 atcatagctt tgtcaagaca ggtagaaata tttgtttctc cctcttcaac aaaatataca
     1201 cctatagatg gaattaaatt gagttctact cccaaacttg tttttatcat tgacaatttt
     1261 ttacgtataa gttcacatat ttctataatt gactctctat tatttttctt ttcaaatatt
     1321 atagcaaaat tgtcatttga aattcttgaa aatacagatt gctctttaaa tatattctta
     1381 attatctctg ctatttcctt taatactcta tctccttctt catacccaaa cctatcattt
     1441 actagcttaa acttatctat atcaaaataa attaaaacta gattcttttt ctcatgagag
     1501 gataaaaatt ttcccccttc aagattaaat ttatttgaat tgccaatacc tgtaacgtta
     1561 tctttaaaag caaattcttc caatttttcc ttactctttt tatgtataaa tagtatataa
     1621 attgttaatg ccgtaaagat tataatcaag actacacatg ctgataatgt aagttttata
     1681 atagcatatg atttctcaga tatagcagtt ttaggaactt cagaaaaaag ataccaatca
     1741 ttgattccta ttggggcata acttaaataa tatccttttc caaacatact ataacttaat
     1801 gaacctgatt taaagttttt tatatttgac ttcatattat tgatactaga ttttgatatt
     1861 gaatcacttc tgccaatgta agaaaataaa ttttctttag tagagttggc gtttttactg
     1921 cttggattta aaacaatatc accattactt ctagttatat aagaatatcc ttttccatta
     1981 aatgtaggta cagaaagtat tttttcatac aacttcgtac tatgtgtggc aaatataact
     2041 cctttaacct cattatcttt ataaattggt acagaatata cattaatatt actattggta
     2101 tttttatcat cttctattac atcttttaat tttcctgtta taaccacttc tccttgcatt
     2161 gattttaaga aataatctct tttagagaag tcttctatat aaccatctgt agtgtatgct
     2221 gtaccatttg gtaaaataac tcccattctt ttaaatgaac tattaatagc ttgtcttttt
     2281 agtatagaaa ggatattatc tacttcaaaa gactcttctc cttcaataaa tattgaaatt
     2341 gactctagta actttatatc tccattaatt tgttttttta acacattgac gctttgattt
     2401 gctatttctg aaagatattg attcgtttct tcacctaatg tcttattcaa ccttgaacca
     2461 taaatcaaac aggaaattgt aattactaag atacttatga cagcaattat ggacaaacca
     2521 tacctattat tttttgcatc acttaacatt atgcttcctg cctttctcga atctatgtat
     2581 gtataatcat tatcttatga caattattgt aaacatttta taataatact tatttttcca
     2641 tatttactgt aattatacaa aattataaac tttattacaa caataaatta attaatatag
     2701 atattagtgt ttatattatt gcattatcta ttttacaatt atttacatat aaaaaaatgt
     2761 aatatcaaag tgaagcttga tattacattt ttgttcttgt attttgttta atttttatgc
     2821 tacatcttac ttaaacactc atttgcaact tctttatcat caaaatgatg cttaacttta
     2881 cctataattt gataagtttc atgtccctta cctgttataa ttacaacatc atcctttttt
     2941 gccatactta tagcttctgc tatagcttgt tctctatcaa caacaacatg gtaattttct
     3001 tttttcttgt caattccttc aagtatatct tttattattg cctctggttc ttcagttctt
     3061 ggattatcag aagttataat acataaatca gaatattttt gtccaattgc tcccatcaaa
     3121 ggtctctttt cagtatctct atctccacca cacccaaaca cagaaattat ttttccttct
     3181 gcaaattgtt gagttgtctt tagtacattt tcaagtgcgt caggagtatg agcatagtct
     3241 acaattacgc ttataccttt atcatttgga actgtttcaa accttcctga tactccatta
     3301 gaaagtctaa ggccttcttt atatattggt ttaggtatgc caagtacata gcaagcagct
     3361 ataactgcta aagtattata tactgtaaac attcctggaa ctggtataaa tattacttct
     3421 tcatatgaag gtgttattaa tctataagat actccatccg aatctgattt tatatctcta
     3481 gccataaaat cagccttagt atcaacacca taagtgtaac aaggtacatt tatacctttt
     3541 atattttcat atatcttttt tccaccttca tcatctatgt ttattatatt tgccatagta
     3601 gtcttaaaaa ataatttttc ttttgctttt ctataatcct ctaaatcttt atgaaaatct
     3661 aaatggtctg gtgttaaatt tgtaaatatg cctagtttaa agtctgtttc atcaactcta
     3721 tttaatgcca atgaatgtga tgaaacttcc atagcacagt agtcacatcc attatccaac
     3781 atattattaa aatgatattg taaatctata ctttctggag ttgttgaatt ggaaacaatg
     3841 tctttttctc catcaaaaat ttttatagtt cctataagtc caactttatt cttatttaaa
     3901 gttaatatct catttagtat agtagttata ctagtttttc cattagtacc tgtaactcct
     3961 ataacgttaa atttttgaga tggatgattg taaaaattat cagctacttt tgccatatct
     4021 tttcttgtat cttttacctt tataaatgta catcctttta tttctacatc ttcttcaaca
     4081 agaaatgcac ttgctccctt ttctatggca tcttttatat acttatgacc atctgacaca
     4141 aatcctttta tacatataaa taatgtacct tttgtaacct ttcttgaatc atattgtaca
     4201 ttattaatat ctatatttaa ttcacccttg acattgatta tatccaaccc ctgtataata
     4261 tcatttaatt tcataaatgc tttcctccct ttaaactctt tctgctaaaa ttacactaca
     4321 tataatatta tattaagaat tttttactat gtgaatggtt aaaaatgctt gaaacattgc
     4381 aaaaaatgct ctataatttt ctataatata actgaggaaa ggtcttctat aaaaactatg
     4441 gataataaaa tcgggtactt aaacgataac tttaaaatat ttaatattag agataaaaaa
     4501 gatatacaat ttgaatacca taatcatgat tttaataaga taattatatt tataaatgga
     4561 aatgtaactt attttgttga tggtattccc tataaactaa agccttggga tatactcttt
     4621 atcagcaata atgaaataca taaaccagtt atagattcaa atgtctttta tgagagaatt
     4681 gcaatatgga ttagcccaga atttatgaaa aaaaatagtg atgctaatag tgacttagaa
     4741 aaatgtttta aaatagctac agagaataaa tgcaatagat tgcgtttaaa tatacatgat
     4801 attgaatcta ttaagagttt tattgagcaa ataaatattt ccgaatccaa tactgaattt
     4861 ggaaatgaaa tattgagaaa tacattattt atacaactta tgatattctt aaacagaatt
     4921 ttcctaaaaa atgaaaatat ggaatcaaca gaagacgttt tttatgatga aactattaag
     4981 aaagtattaa actatataaa ttcaaatata gatgataagc tttcaataga tagcatatcg
     5041 tctgaatttt ttataagtaa gtattatctt atgagaaaat ttaaatctca tactggaacg
     5101 tctatacaca attatatagt tcaaaaaaga ttaattttgg caaaatcact aataaccaat
     5161 ggtcacctta tgagtgaagt ttgtaataaa tgtggattca acgattattc tagttttgta
     5221 agggcattta aaaaaaacta cggtgtatct cctaaaaatt atataaacaa taaaaatcct
     5281 ttggatatta ctcttttaga tgaatagata atttatgtaa attttatttt aataaaactt
     5341 gaagtattag tgtatttatc ttactaatac ctcaagtttt ttaatgtttt aaa
//
LOCUS       DAENCV010000196         5353 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.199, whole genome shotgun sequence.
ACCESSION   DAENCV010000196 DAENCV010000000
VERSION     DAENCV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.199"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            84..464
                     /locus_tag="KQD82_003620"
     CDS             84..464
                     /locus_tag="KQD82_003620"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG4382058.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            479..940
                     /locus_tag="KQD82_003621"
     CDS             479..940
                     /locus_tag="KQD82_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG4382059.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            961..1551
                     /locus_tag="KQD82_003622"
     CDS             961..1551
                     /locus_tag="KQD82_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382060.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1566..2723
                     /gene="alr"
                     /locus_tag="KQD82_003623"
     CDS             1566..2723
                     /gene="alr"
                     /locus_tag="KQD82_003623"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG4382061.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2756..3043
                     /locus_tag="KQD82_003624"
     CDS             2756..3043
                     /locus_tag="KQD82_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG4382062.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3012..3395
                     /locus_tag="KQD82_003625"
     CDS             3012..3395
                     /locus_tag="KQD82_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG4382063.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3566..4393
                     /locus_tag="KQD82_003626"
     CDS             3566..4393
                     /locus_tag="KQD82_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG4382064.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4386..5327
                     /locus_tag="KQD82_003627"
     CDS             4386..5327
                     /locus_tag="KQD82_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG4382065.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
BASE COUNT         2126 a          566 c         1080 g         1581 t
ORIGIN      
        1 ataataatgt gtattatcga tagtaaattt ataaattcat tattaattat tgaaagaagt
       61 attataaatt ttctggagga acaatgaata tatttgatat tggcgtagat ataattgaaa
      121 ttgatagaat aagaaaggct gtagacaaaa ataataggtt tttggagaag atatttacag
      181 atagagaaat agagtatttt aattctaaga attttaaagc agaaagtata gcaggtaatt
      241 tcgcagccaa ggaagctata agtaaatcta taggtacagg aattagagtg tttaatttta
      301 aagatataga agttctcaga aatgaaatgg gaaaaccaat agtaaaaact tataataatc
      361 tagcaaaaat gtgcatagat tataatgttt tagaaataaa agtttcaata tcacattcaa
      421 aagattatgc tatagcaaat gcgataacca taattaagga ctaaggagtg attacattat
      481 gatggataaa acagcaaaag aaataatgac aacagatgta atagtagcaa aacaagatga
      541 tagtatagca gatgtagcta atatgctaat tgcagaaaaa ataggaggtc tgccagttgt
      601 agattcagag aatagagttg ttggaataat ttctgaaaca gatattctta aaaaagaaaa
      661 atatatagaa gcacctctct atataaatct attacaagga ttgattttct tagatgactt
      721 aaaaaaagtt gaaaaagata taaaacaagt agctgcttat aaggttggag aacttatgtc
      781 taaagatata ataaaggttc atgaagatga taagtttgat gatgtggcta atgtaatgat
      841 taaaaaatca ataaacagag ttcctgtagt agatgatgac aacaaactta aaggtataat
      901 ttgtagatat gatataataa aagctttata caatgaataa actgaagtag gaggtgtgat
      961 gtgagaaaaa agtggaccat agtatgtatt atgttcttgg cactggttat aattgtaata
     1021 ggatgccaaa aacgacagtc cacaaaagaa gaagtataca aagattttca aaagcagatt
     1081 tctgatatga attattacag ttgtaaagct gaagtagaag tagttggaaa taaaagtcca
     1141 cacaattatg ttttaattca tacttataaa aaaaccgata actataaatt agaagtaatt
     1201 tccccaaaac atttaaaagg gaaaagtata gaatatcaag gtgataaaat attagttaaa
     1261 aatccaaaga ttagtgatgt agtggagtta cctaatacag gaaaaaataa tcaatatctt
     1321 tttgtaggag actttataaa aaactatctt caaaatgagg agatgaaagt aaagttatcg
     1381 aaaggacatt tggttttaga gacttttatt cctggggata ataaatattt taacaagcaa
     1441 gtattatatg taaatgctga cacaaaaaat cctgaaaaaa tggaagtgtt agataaagag
     1501 ggagtgccaa gatttacagt aaaatacaaa gattttgaat acagaaacta agggggacta
     1561 aagacatgca aaaaataaca gtgcctacat gggcagagat aaatctagat aacttaagat
     1621 ttaacttaaa taatattaaa aatttattag aagaagatat taagatttgt ggagtaataa
     1681 aagctgatgc atatggacat ggtgcagtag aagttgcaaa attgctagaa aaagaaaaag
     1741 tagattactt agcagtagca agaactgctg aaggaattga acttagacaa aatggcataa
     1801 cacttcctat tttgaacttg ggatatactc cagacgaagc ttttgaagat tctataaaaa
     1861 ataaaataac tatgacagtt tattctttag aaacagcaca aaagataaat gaaattgcaa
     1921 aatctttagg agaaaaagcc tgtgttcatg ttaaaataga ctcagggatg actagaatag
     1981 gtttccaacc taatgaggag tcagtacagg aaataataga attaaataaa ttagaatata
     2041 tagatttaga aggtatgttt actcattttg ctacagctga tgaagtaagt aaagagtaca
     2101 cttataaaca agctaataat tataaattta tgtctgataa attagatgag gctggtgtaa
     2161 aaatagctat aaaacatgta tcaaacagtg cagctattat ggattgccct gatttaagat
     2221 taaatatggt aagagcagga ataatattat atggtcatta tccatctgat gatgtattta
     2281 aagatagatt agaattaaga ccagccatga aattaaaatc aaaaatcgga catataaaac
     2341 aagttgaacc aggtgtagga ataagttatg gactaaaata cacaactaca ggtaaagaaa
     2401 caatagctac agttccaata ggatacgcag atggatttac tagaatccaa aaaaatccaa
     2461 aggttcttat taagggagaa gtgtttgatg tagttggtag aatatgtatg gatcaaataa
     2521 tggttagaat tgacaaagat atagacataa aagttggaga tgaggttata ctatttggag
     2581 aaggcgaagt tacagctgag cgtatagcta aagacttagg aactataaac tatgaagtgt
     2641 tatgtatgat atcaagaaga gttgaccgtg tttatatgga aaataatgag cttgtacaaa
     2701 taaacagtta tttgctaaaa taaaaccatg atattataat ctgggaggcg attttgtgta
     2761 cgataagagt aaagaaaaaa tagaagttac tttacctgac tctctgattt ctgaagttga
     2821 tagtatagtt cagatggaga acagtaacag agaagatttt gcaaaagctg cttttcagtt
     2881 ctatataact cagaaaaaga aaattcatat taaagaatct atgaaaacgg gttatagaga
     2941 aatgggtcaa attaatctgt cactagctga attaggtatg acagttgaag aagtatcttc
     3001 tgatgataaa aatgaaggga agattgccca aggtgagtac taattgtgaa ataaagcgtg
     3061 gtgatttata ttacgctgat ttaagtccag ttgttggttc agagcaggga ggcgttagac
     3121 ctgttctgat aatacaaaat gatgtaggta ataaatatag tcctacagtt atagtatcag
     3181 ctataacatc tcagattaat aaagccaaat tacctactca tgtagagata agttccaatg
     3241 aatatgggct taataaagac tcagtaattc ttttagagca aattagaact atcgataaaa
     3301 agagactgag ggaaaaaatt ggttgtctag atgaaaatat gatgataaaa gtagatgatg
     3361 gacttcaaat aagtttagga ttgtttactt tttaaagagg gatttgtctt ttattagaca
     3421 atcccttttt tcttttaaat atctcttaag gtttaatttt aatgtactat gttaataaaa
     3481 ttaaaaataa cgcaaaaaaa tagcataaaa gtgatataat aatatggaat tgatataaaa
     3541 ttacgaactc aagggaggta attgcatgag agaccataaa ggattaaatg agattgcgcg
     3601 tatcataaga agagatatag tttcaatgat acatagagcc aaatctggac atccaggagg
     3661 gtcattatca gtagttgaaa tacttactgc gttatacttt gatgaaatga atgtggattc
     3721 ttcaaatcct aaaatggagg atagagatag atttgtatta tcaaaaggac atgctgcacc
     3781 agcattatat gctacattgg ctgaaaaagg ctattttgat aaggaagaac tgaatggatt
     3841 aaggaagata ggaaggatgt tacaaggtca tccagacatg aaaggcacgc caggtgtgga
     3901 aatatctacg ggttctttag gtcaaggttt ttcagttgca tgtggtatgg ctatggcatc
     3961 aaagttggat aatgctccgt ggaatgtgta tactttgctt ggtgatggtg aggttcaaga
     4021 aggaattgtt tgggaagctg ctatgagtgc tgcacattat aaacttgata atttaattgc
     4081 ttttttagat aataatggac ttcaaataga tggagatata gaaagcgtaa tgagtttagg
     4141 ttcaatagtt gataaattta aagcttttgg ttggaatgta attgagatag atggacatga
     4201 ttttgaccaa atatttgcgg ctcttgatat agctaaatca acagttaaaa aacctacaat
     4261 gatagttgca aaaactataa aaggcaaagg aatatctttt atggaaaatc aagcaggatg
     4321 gcatgggaca gctccaagtg atgaggagct agaaaaagcc ttgcttgaat taggaggtgc
     4381 tgacaatgag taaaatggcg acgagagaag catatggtaa agcattagta aaattaggtc
     4441 aaataaatga caatgttgta gtattagatg cagatttatc aaagtctaca aaaacacatg
     4501 atttttacaa gtcatttcca gatagatttt ttaatatggg tatagctgaa caaaatttaa
     4561 taggcgctgc ttgtggatta tcaactgctg gtaaaatacc ttttgcaagt acttttgcta
     4621 tgtttgctac gggaagggct tttgagatta taagaaattc agtttgttat ccaaagctaa
     4681 atgtaaaaat atgtgctact catgcaggtc ttactgttgg agaggatgga gcatctcatg
     4741 aaagtgtgga agatattgct ataatgagag ctataccaaa tatgacagtt cttgttccag
     4801 ctgatggagt tgaaacagaa aaaattattt ttgaaatagc taagtataat ggacctgttt
     4861 atgttagatt agggagaagt tcagtaccag ttttatttga tgaagattat aaatttgaaa
     4921 taggaaaagg tacagtctta agagaaggaa aggatgtatc aattatagct tgtggaatta
     4981 tggtaaatga agctctatta gcacaagaaa agttgcaaga agaaggtata agtgctagag
     5041 ttataaacat gtcctcgata aaacctatag acaaagattt aatattagaa tcagcaaaag
     5101 aaacgaatgc tattgtaact gtagaagaac atagcataat tgggggatta ggttctgctg
     5161 taagtgaggt agtaggagag tcttgtccaa ccattgtaaa gaaagttgga attaaagata
     5221 catttggaga atctggtact ccaaatgaat tgttaaaaaa atatgaatta acttgtgatg
     5281 acatagtaaa gactgttaaa gaagctatta ttgcaaaaag aatgtaagag atataaatta
     5341 ttcatgtttg tta
//
LOCUS       DAENCV010000197         5339 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAENCV010000197 DAENCV010000000
VERSION     DAENCV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.200"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            104..1081
                     /locus_tag="KQD82_003628"
     CDS             104..1081
                     /locus_tag="KQD82_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382066.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            1082..2053
                     /locus_tag="KQD82_003629"
     CDS             1082..2053
                     /locus_tag="KQD82_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG4382067.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            2205..4247
                     /locus_tag="KQD82_003630"
     CDS             2205..4247
                     /locus_tag="KQD82_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG4382068.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSIAQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            4508..>5339
                     /locus_tag="KQD82_003631"
     CDS             4508..>5339
                     /locus_tag="KQD82_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG4382069.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     V"
BASE COUNT         2132 a          549 c          928 g         1730 t
ORIGIN      
        1 aaaaaaataa taaaatgtaa gaaaatgttg taatttaaat ttttataaaa tacaatcaaa
       61 aggtgtatgt tatcttaagt aattttgctg agaggagaaa ataatgagta aagcactaat
      121 tgaggttaaa gatttaaaag tttattttca tacagataaa gggattgtaa agagtgtaaa
      181 tgaagtaagt tttaatataa atgaaggtga aaccattgga atagttggag aatcaggatg
      241 tggtaagtct gtaacagcca tgtctttgat gaagttactt ccaacatcaa aaatagaagg
      301 tggagaaata atctttcgag gcaaagatat tttaaagatg aatgaagatg aacttatggg
      361 aataagaggg aatgagatat caatgatttt tcaagaacca atgacatctt taaacccagc
      421 gtttactatt ggaagtcaaa tcattgaagg tattatgata catcaagatt tatcaaagga
      481 agaagctaag aaaaaggtaa tcgatatgat aaaactggta gaaataccaa gagctgaaga
      541 aatatataat tcatatccac atgaattatc aggtggtatg agacaacgaa taatgatagc
      601 tatggcttta tcatgtaatc caaaattact tatagctgat gaaccaacaa cagctttaga
      661 tgttactatt caagctcaga ttttagatat aatgaaaaat ataaaagaaa aattaaacac
      721 atctataatg atgattacac atgatttggg agtagttgca gaaatgtgtg acaaagtatt
      781 agtgatgtat gctggcaaaa ttatagaagt ggcagaggtt gttgaattat ttaaaaatcc
      841 aaaacatcct tatactattg gacttctaaa atcaaagcct gctcttggaa aaaacaaaga
      901 taaaagattg tattcgattc caggacaagt accaaatcca attgggatgc cagattcatg
      961 ttattttagt gatagatgtg aaaaagtatg tgataaatgt agaactcaga tacctccttt
     1021 aatagaattg aatagtggtc atagtattgc atgttggtta tataaaaagg aggagatata
     1081 attgaaaaaa gaagagagtt tattaaaagt agagggtctt aaaaagtact ttgaagttaa
     1141 atctggatta tttgataagg aaattaaatg tgtaaaggca gtagatggaa tttcttttga
     1201 tttaaaaaaa ggagaaactt tggctatagt tggagaatcg ggatgtggaa aatcaactgc
     1261 aggaagaacc attcttagat taattgaaaa aactgaaggt aaagttgaat ttaatggtgt
     1321 agatatatat aagttagaga aggaagaact tagaaagtta agacccaaaa tgcaaatgat
     1381 atttcaagac ccatatagtt cattaaatcc aagattaaat gtggaacaaa taatatccga
     1441 ggcagttata gaacataatt tagtgccaaa gaatgaaata aaagataata tattaaatgt
     1501 tgtagaaagt tgtggtcttt ctaagtacca tttaaataga aaaccaggtg agttttctgg
     1561 aggtcaaaga cagagaatag gtatagcaag agcattagca ttaaatccag aatttatagt
     1621 ttgtgatgaa cctgtttctg cactagatgt ttctattcaa tctcaaatta taaatttact
     1681 tatggattta caggataaat ataaactttc atatttattt atatctcatg atttgagtgt
     1741 agtaaatcat attgcacata gagtttgtgt tatgtatcta ggttctataa tagaaattgc
     1801 aactaaagga gaactttact tacattctca acatccatat acaaaagcgc ttatgagcgc
     1861 agtaccaata caagacccta caattaagaa aaatagaatt atattaaaag gagacatacc
     1921 atctccaaca aatccaccaa gtggatgtaa gtttcataca agatgccctt attgtaagga
     1981 aatatgcaaa aaagagattc cagttttaaa agaaatatca agaaatcatt ttgttgcatg
     2041 tcatctagta tagatatttc aaaaattaaa tttgatgtat ataataagaa taatagaata
     2101 aactgaaaat ttttttgatt aagatgggat ttcatgctgt taatattaag ttagttgata
     2161 tataagttta atctataata ttattattga aaagagaggt agttatgtca attttagtaa
     2221 cattgttggg attattggtt tgtacaacga tttcaactgt atttagcaaa aagtggagta
     2281 atattcctct tgcaatatat caaattgtac tagggattat attgtctatt ttgccattta
     2341 aattctcatt ttcatttaat ccagaaatat ttgtaatatg tatcatagct ccattactat
     2401 tcagtgaagg acaaaatgta tcgagaaaag agctattaga gctcagaaaa ccaattttgc
     2461 ttcttgcatt tggtttagtt ttaataaccg tgtttgcagg tggaatcttt atacattttc
     2521 ttattcctgg gatgccatta tctgtttcat ttgctttggc agcagtaata tctccaactg
     2581 atttagtagc agtaaaatca attgcacaag gattaaattt tcctaaaaat atgatgagta
     2641 tacttgaagg tgaatcttta ttaaatgatg ctgctggagt agtagcattt aaggttgcag
     2701 tacttgccac agttacagga gttttttcaa tagaagaggc agggattcaa tttatgatta
     2761 cagcttttgg aggaataata gtaggaagta ttttaggata cataattata aaaataagac
     2821 ttagtttaca caagtggaat ttggaagaaa ttccaatggt aatagttata caaataatga
     2881 ctccactttt tgtatatttt gtagcagaag aaataggagt atctggaatc ctagctgttg
     2941 taatggcagg gatagctcat ggaattgaaa aagagcattt acaaaataca actactaaat
     3001 taaggataat atcagacaat acttggtatg ttttggagta tgtattgaat ggatttgtct
     3061 ttacactgct tggttttttg ttaccaagta tatatacagg acttagtagt aaaaatgaaa
     3121 atatggcatt ggaattgact tttatttcta ctttaattgt acttatttta tttgttataa
     3181 gatttatatg ggtatattta tggcataatt cttttataaa acctaaaaag aatcctttaa
     3241 ataatttttt tgtaggtttt ttaggtttta aggatgaaga agttgtaaaa gagagtattt
     3301 ctaaatgtaa atactcacta atagttgcta cttgtggtgt tcatggtaca tttacactag
     3361 caactgcctt atctatacct ttttatttgg ctgataaaga tgtatttcca atgagagata
     3421 ctgttttatt tatatcatca gaagttattc ttataagttt agtattggca acagttttac
     3481 tgccaagatt attaaaaaat aacgtacaag agtcagaacc tttattatct gataaagagg
     3541 catataaact aattttgaaa gaagccattt taaaacttag taatgaaaaa gcacaagaag
     3601 tacaaccagt tatacatgat ttaaatgaac agttggtaga ctcagaaaaa ggaatactaa
     3661 atactccaga taataaactt ataagtatga tgattgcaga agcagaccaa cagggattat
     3721 tagctgtttt gaaactatta gaagatggta aaatttctga tagagccttt aaattgtata
     3781 gattttatat tacaaaatca agaaaatctg ttcaaaaatc tatatttaaa attgtaaaat
     3841 taaaaataag catgtggatt ataaatagaa aagtaaagaa agaagtaaaa gaaaaaatta
     3901 caaaaatagt atctgagaat aaagaaataa tcaaagagtt tcaccatgca tatgcacttt
     3961 ctactaaaac tgctatatca tttataaata aaaatactga tgaagataac agacatgagg
     4021 ccttaatagc aatcagattc tataatcgtt atttgaattg gattactaga aagattcaaa
     4081 atgatgataa gtttgaggca agagtagaat actacagagt aatggcgata caaagtcaaa
     4141 gagacagtgt tcaacaatta gttgaaacta agcaaatatc aagagaagtg gctaaagagt
     4201 tattggaaaa tattctctat gatgaaatga tgatgataga atcataaata taatggaata
     4261 aaatttgatt tttgatgtta aaattaatac taaaaaatag gaaatagttt tcaataattt
     4321 acttgacata tatatttatt tataatagaa taaaaccata aattgaatac aatatataag
     4381 cgttaacaag ctggattgtt actggtaagc aggcaatacc taggttaagg tatacaggtg
     4441 aattgtatct taatttaggt atttttttat gaataaggga aattgtattt tgggagagat
     4501 attgattatg attattcaac aaatttataa taataatgtt gtattagttt tagatgaaaa
     4561 tataaaaaaa gaattaatat taacaggatg tggaattggg tttcaaaaaa agaaaggtca
     4621 agaaatagat aaaagtaaaa ttgaaagaac ttttgtaata caagatgaaa gctttttaga
     4681 taaaattagt aaactagcaa gccaggttga tgagaaattt tttgaggtta gtactgaaat
     4741 aattgcttat gcagaggaga atttgaatac taaactttat gaatatattt atgtagcact
     4801 tactgaccat attgcatttg caattaaaag atatcatgaa aacataacta taaagaatga
     4861 tttactacat gagataaaaa gaatacataa aaaagaatat gagattggga aatgggctgt
     4921 agattatata aataaagaat ttgatgtgaa atttccagta gatgaagctg gttttatagc
     4981 aatgcatata gtaaattcaa actataaggg aagttcaaag gaatcactgt taattactaa
     5041 aattgtaaaa gacatactaa atattataag atattattat agagttgaat ttaaagaaga
     5101 tgatataaat tatgataggc ttctgactca tttaaaattc tttgcaaaac gattagtaaa
     5161 aaaagaaaaa atcaatgata caaataatga aattatagat ataattaaag taaagtatga
     5221 aaaggattat gattgtgcat ataaaataaa aactcatgta gaaaagaatt atgattacta
     5281 tgtaagtcaa gatgaactac tttatttaac attacatatc aaaagagtta tatctgtac
//
LOCUS       DAENCV010000198         5335 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAENCV010000198 DAENCV010000000
VERSION     DAENCV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.201"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            95..1585
                     /locus_tag="KQD82_003632"
     CDS             95..1585
                     /locus_tag="KQD82_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4382070.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1865..3361
                     /locus_tag="KQD82_003633"
     CDS             1865..3361
                     /locus_tag="KQD82_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4382071.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3495..4421
                     /locus_tag="KQD82_003634"
     CDS             3495..4421
                     /locus_tag="KQD82_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4382072.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4539..4730
                     /locus_tag="KQD82_003635"
     CDS             4539..4730
                     /locus_tag="KQD82_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382073.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4953..5303
                     /locus_tag="KQD82_003636"
     CDS             4953..5303
                     /locus_tag="KQD82_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382074.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2112 a          536 c         1026 g         1661 t
ORIGIN      
        1 atttaaacat ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt
       61 atcgtataaa atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca
      121 caaattaaga catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac
      181 cagagaagca atggataata ttcagtatga aataataaaa ggtgagaaac ctacatacag
      241 atgctgtgtt tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatggggtt
      301 acaaccaggt caagataaga tagatttaga tacaatggat atagaagaag atgaacaaat
      361 tatatatatt ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga
      421 tatttgtaga ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac
      481 acatgtcgga ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa
      541 aaaagcttgt caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc
      601 aactaatgcc ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg
      661 tttaaactgt ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt
      721 gatagctcct gtagctagaa agttagttca aaaagagaaa atgtatgcgg tggtagctcc
      781 agctataaca ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa
      841 atcactagga tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca
      901 tgaaagctta gaatttgctg aaagaatgga aaagggagaa gcttttatga caaattcttg
      961 ttgtccagga tttgttggtt atatagaaaa aacataccca aatcatgtag gaaaaatatc
     1021 ttctacagtg tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc
     1081 taaagttgta ttcataggac catgtacagc caaaaagagt gaggcagcta agaaagaatt
     1141 aaaaggagct gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt
     1201 tgatatagac ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag
     1261 aggttttgga gctgctggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg
     1321 tattggtgtt aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat
     1381 gactatggct aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg
     1441 tggttgtata aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga
     1501 tagaaaaaat tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga taaaagattt
     1561 tagtaatata aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa
     1621 tagagttgtt ctaagttgaa gtttaacttt tggacaactc tatttttatt tatatattta
     1681 attattgaac aacaaaaata aaaattatat acacttaaaa taaaaattac atatataata
     1741 ttttgtatat aacttaacaa agtatatgcc tatgttataa taatttatag aaataaataa
     1801 aaaatacaat atacataata gtagaaatat agcaaaaagg tgtgatttat agggaggatt
     1861 acttatgttt gaatttgaaa cgcaactaaa aaggttaaaa catgaagttt taacagcagt
     1921 agcaagacta tctattgaag aaaatttaac aaaagaaaat atagaaaaaa ttccatatga
     1981 aataattaaa ggagaaaagg cgagatatag atgttgtgtt tataaagaga gagaagtagt
     2041 tcttgaaagg gcgcaacttg cattaagttt aactccaaat agtaaatatg gagatataaa
     2101 tccagattca gttagtttgg acacagatga gcaaattata tatatagttg aagcagcatg
     2161 tgatagatgt ccaataaatg aatttacagt tacagagctt tgtagagggt gtttggctca
     2221 tagatgtaag gaagcatgta aatttggggc aatttcttat ataaatggga gagcatatat
     2281 aaatcatgat ttatgtaagg cttgtggaat gtgcaaaagt tcatgtcaat atgatgctat
     2341 atcagaagtt gtaagacctt gtaaaagcgt ttgtccaaca ggagctttgg attttaatag
     2401 aaacactatg aaagcaatga tacatgaaga taattgtata aattgtggag catgtatgtc
     2461 agcatgtcct tttggagcaa tatctgataa gagtttgata gcacctgttg caaaaaaact
     2521 tgcaaagaaa gaaaatatgt atgcaatagt agctccagct ataacaggtc aaatagatag
     2581 taatattaca tatggtcagt taaaaaatgc tataaaatca ctgggatttg tagatatgat
     2641 agaagcttcc tgtggagcag atgcagttac agttcatgaa agcaatgaac ttattgaaag
     2701 aataaaatca ggagataatt acatgacaaa ttcatgttgt ccgggatttt ttaagtatat
     2761 agagaagatg ttcccagatg aaaaagatag aatatcatct acagtgtctc ctatggtagc
     2821 aacaggaaga ttagtaaaaa aaatagattc tgatgctaaa gttgtattta taggtccatg
     2881 tacagctaaa aagagtgagg caacagagaa aagtttaaaa gattcagtag attatgtact
     2941 tacttttgaa gaactaatgg cattatttga agcatttaat atagatttat caaaatgttc
     3001 aaatgaagat ataaatgatg gttcgatttt tggaagggga tttggagcat ctggtggact
     3061 tgccaaatca gtacagaact atattgctga aaaggggctt agttttgatt ttgaacctat
     3121 aaaagtatct ggtccaagac aaattaaaaa gacaatgaca atggctaaat tgggaagatt
     3181 agaaggtaat tttatagaag gaatgatgtg tgaagaaggt tgtattaatg gagcaggtaa
     3241 gtttttatca ggtgtaaaag ctaaaaatac ttttgataga aaaaatggac aatctactaa
     3301 aaagagtgtt ttatctaatg ataagattaa agaatttgaa gatgtaaatt tagaaagata
     3361 attgttttgg tacagtcatt tttttattat gccacaaata tgcctaaaat ttgccctttt
     3421 tatatgtgta ttttacagag ttaaggttgc tatactataa atatgtaata aataaaattt
     3481 ataggagctt ttggatgagg aaaatagtat taaaagatat cctgttttta gttactacat
     3541 tatctataat agtaatatta ttgcttgtaa caaatataac tactaatagt aagataaaat
     3601 caacaagtga atcttattta aaaagtgaca aaaatagtgt gaggcaaaat ggtatccaga
     3661 ggagcacaaa gagtagcaca aaaaatactg catttaagac tactatacta aacactggaa
     3721 agtctgattg catattggtt gagataggta acaaagtcat aatgattgat acaggagaag
     3781 ataaaaatgg aaaacaaata gtggataggt taaaagaaaa aggaataaat acacttgact
     3841 atttaatttt aactcattta gataaggacc atataggtgg agtagatagt gtgttatcta
     3901 gtgttaaagt aaagaatatt atacaagcaa attataagaa agatagtaag caatatgatg
     3961 aatatattga ctcacttaaa aaagctgata tagaaccagt gctactaaaa gataatatga
     4021 atattgtaat caatagtgca gaaataaaca tacatccggc ttcaaaaagc aagtacgaga
     4081 gttcaaatga ttattcaata atcactaata taagctatgg agcacataag tttttatttg
     4141 caggagatgc agaggaagaa agattgtcag agtttataaa tggaaatact ttaaaatatg
     4201 attttgttaa aatgcctcat catggaagat atgacaaatt aactgaaaca tttttagagt
     4261 ctatttcacc ccaatatgct gttattacat gttcagagaa aaaagaacca gaggaaggtg
     4321 ttttaaaaat attggaaaga ttgaatataa aaaccttttt gacttctaat ggagaggtag
     4381 ttatcaatag tgatggtaag acattgagtg tgaatcaata atttttttaa aaatatctaa
     4441 aaaaatttaa aaaaatcatt agcaaatatt accccttaaa catagtatat tttaggagct
     4501 gaaactccac atacaaaatg actaggaggt aacatattat gttggataga ttttttggtg
     4561 aaggcggatt tggtggatgt gcttggtgga taataatatt attcttctta ttcttagcat
     4621 tccaagattg ttgggctgaa atggacttat gtgcatggat acctttctta atattattat
     4681 taatactttg tgaatgtggt gacgtttttg atggaggatg tggatgctaa aaaaatcaat
     4741 ttagatgatt taataaagct tatggtgggt ataaataaaa taccaaacca atcacaatct
     4801 agtgtttgaa ggtagtcgga aatcccccgc taccttcttt tttttattgt taaaatttat
     4861 ttttgaatta tttcatttac atataataat ttttacgata aagtctgata taattaaaaa
     4921 gaaaataaaa tttatttata ggagatgtaa aaatggctat agtggataag attttcaatg
     4981 agataaaact tatggttgca agacttttat tgggaaaaaa gtacagtgaa tatgaagaaa
     5041 aagatttagt gtataataca gaagaagaag ttatacttat aactttaaaa agattagttt
     5101 ttcagggaaa tgtaaacgaa gcagaagaaa ttttatttga taaagctaag agtgtaaata
     5161 gtgaaaatat gcagtatata gcaatagaat tttatactat gcttatggaa aagactgatg
     5221 aagaattaga agctatgaat ttttctaaac aagaagttta tcaaggtata gaagatatta
     5281 gaaaagtatt gaatttagaa taaaagatat atgaaaatat tgaaataaat taatg
//
LOCUS       DAENCV010000199         5190 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAENCV010000199 DAENCV010000000
VERSION     DAENCV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.202"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            60..1064
                     /locus_tag="KQD82_003637"
     CDS             60..1064
                     /locus_tag="KQD82_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4382075.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1190..2302
                     /locus_tag="KQD82_003638"
     CDS             1190..2302
                     /locus_tag="KQD82_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4382076.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAES
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2791..3552
                     /locus_tag="KQD82_003639"
     CDS             2791..3552
                     /locus_tag="KQD82_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382077.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3555..4373
                     /locus_tag="KQD82_003640"
     CDS             3555..4373
                     /locus_tag="KQD82_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG4382078.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4553..5155
                     /locus_tag="KQD82_003641"
     CDS             4553..5155
                     /locus_tag="KQD82_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG4382079.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIKPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         2178 a          477 c          930 g         1605 t
ORIGIN      
        1 taggatgtaa aaataaatag gatataaaaa tatcgtaaat gtatgaggag tgaaatttaa
       61 tgcgtttcaa aagaatattg atattgatgt taactgtagc aatgatagct ggaatgttgg
      121 ttgggtgtgc taataaccca agcaataatc ctagctctag caaagataat aaaagtgata
      181 taagcgataa aagtaattca aaaaaatctg tatcaatgat actagatatt gaaggaacaa
      241 ataatgaagc tatgaataat tcagctttat tagcattaaa caatgctcaa aagaaactta
      301 atatagatac aaacaaagtg gaatcagatg attcctcaac tttttctaat agtatagata
      361 ttttatgtaa tgacaattac gatttaatta tagctgtagg agctagattt gcaaaaccat
      421 tggagatggt agccaagaaa tatccaaagc aacaatttgc aattatagac tatgagtatg
      481 acaagcaacc ttctaatata acttcaattt cttatgaaga taataaatct ggatatctag
      541 ctggattaat tgctggaaaa atgactgaaa gcgataaggt tggttttata ggaggaataa
      601 agagctctag tagggataaa tttgaatcag gttttagaga gggtgttaaa ttttctaata
      661 gtagtataaa ggatatttca gttgagtatg cagatgtttt taaagatagc aaatcagtag
      721 aatctatagc taaaaaaatg atggataatg gagtggatat tattttttct actactgaag
      781 atgattcaaa agctgtaata gatgcagtaa gggcaaaaaa taaaaaagtt atagcaacga
      841 ataaagacca acatgagtta gctccagaaa atgttataag ttctattgta aaagattttg
      901 agaatcctac ttataatctg atacaaagtt ttgtaaaagg aaattataaa ggcggaaaag
      961 ttatagaaaa cacagttaag tctggaagta ctgggcttgc ccaaaatagt tctcaaaata
     1021 ttcctccaga tgtgttagaa tatgttaata agaacaataa ataatctact ataatatcca
     1081 agaatactta tgactttagt tatgaggtga attggatgtt ataatagaca tatatagaaa
     1141 gacatatgca gaggtaggtc agacctatct aaatcaggga gtgaattttg tgaaaataaa
     1201 aaggatattg atggcttgct ttgtagtagt ttttacaatt tttatattag ctatatgtaa
     1261 ttttgatttt ggatttgata aagtaaataa gaataattct aatatagata gtgctgaatc
     1321 gaaacagaat gagtctaaaa gtttgccaac ttcagcagaa aaggataaat caaatagcaa
     1381 aagtaagaat aaagaagatg acacagaaga tgattcatat gtaagaaaaa ctattggagt
     1441 attgttagat accgaaaact taaataacga atcatataat aacaatgtac tatcagcctt
     1501 aaaaagggct gacgaagatt ttgatgttag gataaaactt gaaaaaccaa agacttctaa
     1561 aagttttaaa gagagtatag aaaaactgca taagtatgaa ccagatttga taatagcagt
     1621 tggagccaga tttgcagagc caattaaaga agctgctact aaatatccca aacagcaatt
     1681 tgcaatagtt gactatactt ataaagttca gccagctaat gtaatttctg tatcatttca
     1741 agataatgtt actggatatt tagcaggact tattgcaggt aaaatgactg atactgggaa
     1801 agtcggtttt ataggaggag ttgagggttc tagtagagat aagtatgaat ctggatttag
     1861 aaaggggctt aaaacttcaa ataaagatgc tgagttacta gttaagtatt caaatatatt
     1921 tgaaaaccct gaatcagcta gaactattgc aaaggaaatg agagattctg atagagatgt
     1981 tgtattttca gctacagaag atgatagtaa aagagctatt caggtagcaa gagaaaatgg
     2041 tggtaaagtt atagctatta ataaagacca atacgatatg gctccagata atgtaataag
     2101 ttcaattata aaagatgtag aacaacctgt atatgaactg ataaagaacg ttgcaaaaga
     2161 tggttttaag ggaagtaaag taatggaatt taaaataaaa gatggagaac ttggcttgac
     2221 ttcaaagagc agtagaaata taccaccaga tgttttggaa tacataaaaa aagagtataa
     2281 caaagtgaaa gatacatatt aattattgag tagaagatta taaaaatata ctgattaagt
     2341 ttagattggt gataaaagga tatatttatt aaatttgtgc taactgtaca aaatatattg
     2401 attaagttta gattagtgat aaaaagatat atttattaaa tttgtgttaa cagtatggaa
     2461 tatactgatt aagtttagat tggtgataaa aggatatatt tattaaattt agtctaactg
     2521 tacaaaatat attgattgag tttagattag caataaaaag atatatttat taaatttgtg
     2581 ctaactgtac aaaatatatt gattaagttt agattagtga taaaaggata tatttattaa
     2641 atttgtgcta actgtacaaa actatactaa ttaagcttag attaactata taaaattata
     2701 acaataagtg tgtgggcaga aaaattctgc tacacacttt atttatattc acataaaaaa
     2761 tttatcaaaa aaaagaaaaa ggagtaaaaa atggaattta caagagaacc atttatacta
     2821 gatgacgatt tgtgtctagc tttggtggac aaaagaatta gttatctaat ggaaaatgaa
     2881 ttaaaattaa gaaatataga gataataaaa acaatagaat gtaaagaatt atatgaaagc
     2941 ataaaatatc acccggatat atgtatttgt aatttaggaa aaggagatat aattgttgcg
     3001 ccgaatgtat ataatcaata caaagagtta ttaagtagat ataaatttaa tataattaag
     3061 ggagaagcta atttattaaa tagataccct tatgatatac cttacaatat tgcgatagtt
     3121 ggtgactatg ctatacacaa ttttaagtat actgacaaag caatattaga ttatattgaa
     3181 aataataaac ttaaaaagat aaatatagag cagggctatt ctaaatgctc aatctgtata
     3241 gtagatagaa cgtctataat aacatcagat aaaggtattt ggaaaagtat gaagaataca
     3301 gaaatagagt gtttattgat agaaaaaggt cacataaatt tatttgagat gaattatgga
     3361 tttataggtg gatgtacagg gttgatttct aaggataaac tagctttttg tggagatgtt
     3421 aaaaaacatc ctgactatga acgtataaaa agttttgtag aaagtaaaaa caaggagata
     3481 gttacactta gctgtgaaaa tttactagat ataggttcta tagttcctct catgacaaga
     3541 aaggagcgat aactttggtt gagaaggata atatttcttc catgaaattt ttgaggaata
     3601 ataatgtaaa cgatagatta atatatacga atgacattgt agaagtagtg atagattctg
     3661 caaagcaaga agtgttaaat aaaaaaatac ttaatggtac taaagctgaa caaagagctt
     3721 taaatattga tgagatatca acggaaataa ctagtatgat aataagtata atgaaagaga
     3781 aacttttgaa agaatacatt ctaaaatgct atgggcgtac atatcctgaa gataaaaata
     3841 atatatacat atattcactg aacatatttg ctaaaaaaga gatttttatg agagaaacag
     3901 tatttactag ggtgagaaat tatatagaag aaaatgattt tataaatgta gaaggtttta
     3961 ttagatttag aatgagagaa tttatgaaat atatttctgc tataggtgat attgcagttg
     4021 aggagtattt aataaagaaa gaccaagatg aatttataag agttttaaaa tactttatag
     4081 atactcaaga agaaaagatt gatttgctta aagttcatat aatggaagat aatacatttg
     4141 ttttatacga taaaaatgga aataagatag acagtataga tgatgaagaa attataaata
     4201 tggtaataag agaaaattta aattatgagg actttttaat aagtacctta ttaactctat
     4261 gtcccaaaaa aatagaaata ttggatttgt taaataataa ttgctcaaag gaaattatag
     4321 atactgtaga agcaattttt gaaaataaag ttagtataat tatggaaaac tagaatctaa
     4381 gcatataaaa gctatccata taaaattaaa aaaattcttt ttaaatcgac acaaaagact
     4441 tataattgta tataaagcta tagtataact agaattatta agtataaaga atatctactt
     4501 gattaataca atatagctgg caaatataca attgataagg agtaatttta taatggataa
     4561 tttaattagg atactgatat tagctgttat tggtggattt ataggttatg ttacgaatgt
     4621 agttgctata agactgatat ttagaccaat aaagcctatt aaaataccta ttttaaatat
     4681 agaaatagta ggtcttatac caaagagaag agcagaaata gcagcaaatg taggagaaat
     4741 aattcaagaa gaatttcttt ctatggacga aatacttgca aatatcatta ctgacgaaga
     4801 taaggaagaa gttgtaagat acataaaagc aagggtaaaa attataatac atgagaaagt
     4861 ttcatttatc ccgagtggta taaaaaacat gatacaagat tatctaggag agataattga
     4921 gtctgaagtt aagcaaagta tagatgaatt gagtaaaaat ataataaata aagcaaatga
     4981 acgtatagat atacaaaaaa tggtcgaaga taagattaat gaactagatt tatatgaatt
     5041 ggaagagata ataataagaa tagctaagaa agaattaaaa catatagagt ttttggggct
     5101 tgtcttaggg tttttaatag gtatagctca agggttaatt actatgttta tttaaatata
     5161 tgagaagtag catttattta agtactaatt
//
LOCUS       DAENCV010000200         5124 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAENCV010000200 DAENCV010000000
VERSION     DAENCV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.203"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(199..1791)
                     /gene="hcp"
                     /locus_tag="KQD82_003642"
                     /gene_synonym="priS"
     CDS             complement(199..1791)
                     /gene="hcp"
                     /locus_tag="KQD82_003642"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG4382080.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1851..2576)
                     /locus_tag="KQD82_003643"
     CDS             complement(1851..2576)
                     /locus_tag="KQD82_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG4382081.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2741..3445
                     /locus_tag="KQD82_003644"
     CDS             2741..3445
                     /locus_tag="KQD82_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG4382082.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(3609..4880)
                     /locus_tag="KQD82_003645"
                     /pseudo
     CDS             complement(3609..4880)
                     /locus_tag="KQD82_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1679 a          900 c          621 g         1924 t
ORIGIN      
        1 agtattataa atttaataca aaaattctta aaagaatata ctgaaagcag gtttattttt
       61 caattattac aaaaaataaa taaagggtca tctcaaaatt gattttttaa tcaaatttta
      121 agtttaaccc cttatcttaa ttgtggcata cttaaaaata ttttaactat aaatatcttt
      181 aagtatagat atatttattt atcctaaaat cttcttcata tcttcttcag gagtagatat
      241 aggtgtcaat gcaaatttat caaccaatat atctaataca ttcttagata agaatgctgg
      301 caaagaaggc cctatataga tatttttgat acctaaatga agtagtgtaa gaagtataca
      361 aacagctttc tgctcatacc atgacagtac catgctaaga ggcaaatcat ttactccaca
      421 ttcaaaagca tttgccaatg aaactgccac tttaattgca ctataagcat cattacattg
      481 tcccatatcc attaaacgag gtaagccacc tatatcccca aggtctaaat cattaaatct
      541 atattttcca catgcaagtg tcaatataac agtgtcttta ggagcttgtt ttacaaattc
      601 agtgtaataa tttcttccag gttttgcacc atcacaacct ccaaccaata aaaagtgtct
      661 tatagctcca ctctttacag catctactac tttatcttgt acagatgaaa ctgtacctct
      721 tgcaaaacca gtagttaatg tatctcctcc attaatacca gtcatatgaa catcttctct
      781 atatcctcca agttcaagag ctttttctat tacactagaa aaatctttat cttcatcaat
      841 atgaaccata cctggataag caactacttc tgtagtaaat actctatctc tataactatc
      901 tttaacaggc attaaacagt ttgttgtaaa taatattgga gcaggaatat tatcaaattc
      961 tttttgctgg ttttgccatg ctgtaccaaa attacctttt agatgtttat attcttttag
     1021 ttttggatat gcatgtgctg gtaataactc accatgagta tatatattta tacccttatc
     1081 tttcgtttgt tctaataatt tgtgtaaatc ataaaggtca tgtcctgtaa ctacaataaa
     1141 tggccctttc tcgattttca tagatacctg agtcggttct ggtgttccat aagtttcagt
     1201 atttgctcta tcaagcaatt ccatacattt taagtttatt tctccagttt tcataactag
     1261 acctaaaagt tcgtcaatag acaagtctcc tccaactgct attaatcctt catagaaaaa
     1321 ttcatttacg tctttgtctg tataaccaag taccattgca tgatatgcat aggcagccat
     1381 acctcttaat ccaaataaga ttaatgattt taatgaacgt atgtcctcat tatcattcca
     1441 taaatccttc atatcatagt tatcatttct attacaagtt gttgaacaat ctgaacatct
     1501 aggtacaagc ttactttttt ctacctcagt tttttcaatt tgtcttttta tagcttcatc
     1561 atcaaaactt acattcgtta tagtagtgaa tagcccctct atcataattt tatctgtttc
     1621 acttgttggt ctattcttat ttccaatagt tgctcttgaa agaccaatca acactcctgt
     1681 aagttcgtct tgtaagttag ctgtaccccc ctttttacca cagacaccat ttatagtaca
     1741 acctttacct cctgctgttt gttcacattg aaaacaaaac attttttcca ttttttattc
     1801 ctcccttttt atttattaaa atttattata tttttctata attcacttaa ttaaatttct
     1861 ccatctacaa tttttccatc tacagatata gtaactattt gccatggtat catttttcca
     1921 ctattttgta gagctgtctt aacagcattt actataccac cacagcaagg aacttccata
     1981 cgtgttacta ctatattttt aatatcattt tcttttaaaa tctctgtaag tttttcagca
     2041 tagtcaactt catcaagttt agggcatccg attaatgtaa ccttattttt cataaattta
     2101 ttatgaaaat ttccatatgc atacgctgta cagtctgctg ctattaaaag acttgcattc
     2161 tttaaatatg atgcattagt tggtactaat tttatttgta ctggccattg attaagttga
     2221 gaacctttta tctctactat atcattgctt atagacgtac tatttgaaac ctctctattt
     2281 attgacttag actgagaacc tggacatcca catgctaaag ttttcttttg tgctttcttt
     2341 gcttccatat ttgcttttac tgcagcttca tcatattctg ctgcttctct atactcaaat
     2401 gaaatcgcat ttgttggaca agttggcaga caatctccca gcccatcaca atagtcatct
     2461 cttaaaagtt ttgcttttcc atttaccatt cctatagctt cttcatgaca agcttcaaca
     2521 catagtccac aaccattaca cttttcctca tcaattttaa ttatttttct catcatttat
     2581 atcacctcat aaaatttttt taatttaaag tattgatttc ttatatattt atttttaata
     2641 cctatatttc tttatttcaa atttcttgta gtaggatttt tatatcccct tgcttttata
     2701 aacttagtat agtacaatga aaacaaatta tcggttgcat atgcaacaag gaagtgatta
     2761 tttgaaaaaa tttttagaaa agcatataga cctacttaaa aaaattcctc tatttgaagg
     2821 aattaaaagt gatgaacttg aagaaatgtt ggaatgttta ggtgttgtag ataagactta
     2881 cccaaaagat agtataatat tctctgttgg aagtgaagtt acttctattg gaattatgct
     2941 taaaggaagt gctcatataa taaaggaaga tatcgaaggg aataggaata ttgtagctga
     3001 actcttgcct ggagatttat ttggagaggt attcgcatgt actagactac ataaaagtaa
     3061 tgttacagta accacaacat cttcttgcga agttttattt ataaagttta aaagtgtaac
     3121 tggtatttgt tcatctgcat gtgtatttca taataggctt gttgaaaata tgctacaatt
     3181 aatagctgaa aaaaacattt tacttcacaa taaaatagaa ttactttctc gtaaaacaac
     3241 tcgcgagaaa ttgcttatgt atttttcaaa acagatagaa caaacaggta gccaccagtt
     3301 tacaattccc ttttcaagaa atgaaatggc tgatttttta tgtgttgaca gaagttctat
     3361 gtcaagagag cttggcaaga tgcgagatga aggcttgtta tcttttaaca aaaataaatt
     3421 tgaaatcttt tattttaaaa aataaaatag atatttaaac atatttattt caatatatac
     3481 tatattagtt gattcaaatt ataaatatca tatttgaatc ttaaatttat atttttactt
     3541 aaaaagttaa taggtatcta atttattaaa attaagatac ctattaactt tattaattaa
     3601 gtgtacaatt agcttatttt ctttgtagta attgctgttt tatctataag ccaatctttt
     3661 ccttctacta atcttgaaac taacattgat gatactgtat ttccagttgt atttaatata
     3721 gtattaggaa tatcagttat agtagttata actgttaaca gtggaattgc ctccactgga
     3781 aaaccgaaga tgctaaccac gagcatctca ccaataagtc agccacctgc tacaggagcc
     3841 ataaatactc caacaacaaa tacagttata aataattgac ctatcgttgc tggatttgcc
     3901 attccactac ctaaaatacc tgataaaaat gccactttta ttattactgc taaaactgac
     3961 ccatctttat gtatatttat tccaagaggt ataactgttt ctgatatatc atcaggaact
     4021 cctatatttt tactagcttc taaatttgat ggtatacatg ctgaacttga agaagtagct
     4081 agtgccgttg ctattggttt tatagcattt ctccagaatt gtctaaaccc ttgttttcct
     4141 gcagataaaa aagcatacag actgtaaaat aataagaaat acatggctgc tgttagtgaa
     4201 taaattacaa acacccttac atatccttga attattttac ttccaagttc cccaattatt
     4261 gtagctgtat aacagcctag ccctatagga gcataaagca tgaatatact tactactttt
     4321 aatattgctt ccgttccttc tgataaaagc tttcttattg tatttcccct atctcccatc
     4381 atagctattg ctgttccaat taaaactgca aatataactg attgtagtaa attactactg
     4441 gaaaataaat ctccaaaatt tttaactgta atacttgata ctacttggtc taataacgtt
     4501 ccttttgatt ctaaagtttc tgcatcttga gccaccatta aattttttac acttgataca
     4561 tctaatcctt tagacggatt aaataataat gcagatgtaa tacctaaaaa tgcaattaca
     4621 agtgttgttc ccataaatac tatagctgta cccttcatta tttttccaaa tcgttccata
     4681 ttttttatac tagcaacacc tgaagaaaca ctaagaaaaa ctaatggtac tactatcatt
     4741 aatagagcat ttatgaataa atctccaaat ggttttagta ttgaagcttt tggaccaagt
     4801 actaatccta ctatcgcccc tactatagta catgctaaaa gtataattga tgacttataa
     4861 ttttttaaaa agtccctcat ttatattttc ctttctaatg ctttatatta taaattaccc
     4921 caaatgctaa aaaaattaaa gtaaattatc taatgcgcat taataatttt tgtaattttc
     4981 cccaacagct actttagcaa atactggaat atatgtcaat ttctatagtt tttttgtatt
     5041 tttaacgttt tattaatagt tcatatctat atataaatat taataaaatt ctgcttttaa
     5101 aacttttttt ctagattaat ttta
//
LOCUS       DAENCV010000201         5049 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAENCV010000201 DAENCV010000000
VERSION     DAENCV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.204"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            40..1923
                     /locus_tag="KQD82_003646"
     CDS             40..1923
                     /locus_tag="KQD82_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG4382083.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2148..2828
                     /gene="pgmB"
                     /locus_tag="KQD82_003647"
     CDS             2148..2828
                     /gene="pgmB"
                     /locus_tag="KQD82_003647"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG4382084.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2825..>5049
                     /locus_tag="KQD82_003648"
     CDS             2825..>5049
                     /locus_tag="KQD82_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG4382085.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYDEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     P"
BASE COUNT         2083 a          457 c          958 g         1551 t
ORIGIN      
        1 tagattatat aaattattat aaaggaggga aattttaaaa tgtctaataa aaataaagaa
       61 aatataactt taagtaatca aatcaaagga acaattcaaa atagtttaac aaaagaagct
      121 atattacata taccaatgag caattatgcc tatggttatg atagagaaac actacatata
      181 agacttagaa ctaaaaaaaa tgaagcgaaa agagtaattt tacgaatagg agaccaatat
      241 gtgtgggata aaggaggtgc aggaggagga aatttaaatg catctggagt tggttggtct
      301 ggtggaacaa atatagtaat gagtaaagaa gtagaaactg aattatttga ctattggata
      361 gctaagtgta agcctttaaa taaacgctca cgatatggct ttataataga aggtcaagag
      421 gaaaaacttt tatttacaga aaaaagaata atagagttgg gaaatgaaaa tgatgaaaag
      481 gaattatgtg aaataggtaa tttttttgga tatccatatt taaattatat agatatacct
      541 aaagtgccaa attgggtgaa agatactgtt tggtatcaga tatttccaga tagatttgca
      601 aatggtgacc cgtctataaa cccagaagga gtagataaat ggggagctat tccaaccaga
      661 gataatttta caggtggaga tttacaaggt gtgatagaac acttagatta tttgagtgat
      721 ttgggaataa acggaattta cttttgccct attacaattg gaaaaaccaa tcatagatat
      781 gatacggtag attatatgga ggtagaccct acattaggag ataaagagac tttaaaaaaa
      841 ctaatagaag aagcgcataa gagaaatata aaaataatgt tggatgctgt ttttaatcat
      901 ataggatatt attctaaaca gtggcaagat gtagttcaaa ataaggaaaa ttctcgatat
      961 aaaaactggt tttatataaa agatatgagt aaaattgata ctccaataga acaaatagat
     1021 gaaaaaaata taccttatga aacctttggg tgtgaaaaat atatgcctaa acttaataca
     1081 gaaaattcag aagtaataaa atatttattg gatgtaggca agtactggat acaggaattt
     1141 gatatagatg cgtggagatt agatgtttca aatgaagtag accatgtttt ttggagaaag
     1201 tttagagaag aagtcaaaaa agtaaaacca gacatttata tattgggaga aatttggcat
     1261 ggtagtttac catggctaat gggtgaccaa tttgattcag ttatgaatta cttgatgtca
     1321 gaagccatga aaaaattctt ttgtacagat gagataaacg ccgaagaatt taagtatatg
     1381 ataaatgatg taatggtaag ttatccaata caagttagtg aagttatttt taatttactt
     1441 ggaagtcacg atactacaag aatattgact tatgctaatg gaaatattga tagatttaaa
     1501 cttgcatata tgtttatgtt tgtacaaaca ggatgtcctt gtatatacta tggtgatgaa
     1561 atcggaatgg aaggagaatt aagttcaata tctgaaggtc aaagaaaatg tatggaatgg
     1621 aatgaagaaa aatggaataa ggatatattt gattttatga agaagattat tagacttcgt
     1681 aaagaaaata aagaacttag gtctatatca aatgaatggg tgttagcaga taaggaaaat
     1741 ggaacgatta tattgaaaaa agaatctatt tctataataa taaataattc atctaaaaat
     1801 ataattttga cgttaccaga ttatttagca aataaaaaag taaaagattt atatgaagaa
     1861 gaaatagttg atttaaaaga agaactaatg ttagagaaat acaagttcat tattttaaaa
     1921 taatacaaaa aagtatttat tttagatttc atgttaaatt attatacagt ttaaataagt
     1981 ctttataaac gataaaaaaa tatggtctac aagtgagtga attgaaagta gattttaaat
     2041 taaagactga gttagagtag ataattttat aaataatatc ttaaatacaa gatagaaaat
     2101 aaattgatta ttagtctaaa aatttcttgt agaaagagga aataagaatg attgaagcat
     2161 ttatatttga tttagatggt gtaattacag atactgcata ttatcactat atggcttgga
     2221 gaaaactagc ccataaagtt gggatagata tagacacaaa atttaatgaa tctttaaaag
     2281 gcataagtag aatggagtca ttggacagga tattagaatt tggaaataag aaatattcat
     2341 tttcagaaga agaaaaagtg agaatggctg aagaaaaaaa taattactat gtatcattga
     2401 tagatgagat aacttcaaat gatattttgc caggtataga aagcttgtta attgatgtaa
     2461 aatctaataa tataaagata ggtctatctt ctgcaagtaa gaatgcaata aatgtattaa
     2521 atcatttggg tataagtgac aaatttgatt ttatagctga tgcaggaaaa tgtaaaaaca
     2581 ataaaccaca tccagagata tttcttatgt cagcgaaagg gcttaatgta aatccacaaa
     2641 attgcatagg gatagaggat gcaagtgcag gtatagacgc tataaactca gctaatatgt
     2701 tttctgttgg tgttggaaat tatgaaaact taaaaaaagc aaatttagtt gtagattcaa
     2761 caaatcaatt aaagtttgaa tatatacaag aaaagtataa tgagtatata gttaggagaa
     2821 ttatatgaaa tgtttattta gtaatgtaga tgagtggaag attatacaag ataagattga
     2881 aatcaaagaa aatagattag cagagtccat aatgagtata ggcaatggat atatgggtat
     2941 gagaggaaac tatgaggaaa attatagtgg agatacacac aaggggtctt atatagctgg
     3001 agtatggttt ccagacaaaa ctcgtgttgg ttggtggaaa aatgggtatc cagaatactt
     3061 tggtaaaatt cctaattcag tcaactatat aggtgtaaga attttcatag atgatgtaga
     3121 attagattta gcaaaatgta aagtagaaga tttttataga gaattagata tgaaaaatgg
     3181 tattttaaat cgtaggttta ttgtaaatat aaatgataaa aaatttgaag ttagggttgc
     3241 taggttttta agtatatctg ttaaggaact tgcagtaatt aaatacagta taaaatcatt
     3301 aaattctaat actaaaataa aatttgtacc atatttagat tcaaatataa aaaatgaaga
     3361 ttcaaactat gatgaaaagt tttggaatgg aatttcttca agttctttag aatataaagg
     3421 tagtattctt tctcgaacaa aggagaatgc atttaataca ccagtattca ctgtgggttc
     3481 aatgatgtat attaagacaa atggtgatgt agaagctgta tctcatgaga gtgattataa
     3541 ttactgtgaa aattcgataa caatggatgt tttaaaaaat gaggttgcaa gtatagaaaa
     3601 atatgtatct ataacttcaa ctagagatta taaggaaagt gagctattaa gaaaatgtga
     3661 agacatttta aatagagaac tatctaaaag ttatgatgat atattaaatg aacatacaaa
     3721 gttgtggaat aaaagatggg aaagtgcaga tgtaaaaata aatggagata aaagtagcca
     3781 acaaggtata agatataatt tattccaact attttcaaca tattatggag aagattcgcg
     3841 attaaatata ggtccaaaag gatttactgg tgaaaagtat ggaggagcta cttattggga
     3901 tacagaagcg ttttgtgttc cagtatatct aggtgtagca gataaggagg taactagaaa
     3961 tcttttagaa tataggtaca atcagttaga acaggcaaag gataatgcaa aaaaactagg
     4021 gcttaaaggt gctttatatc caatggtaac atttgatgga gtagaatgtc ataatgaatg
     4081 ggagataaca tttgaggaaa tccaccgaaa tggaagtata gtatatgcaa tatataacta
     4141 tacaaattat acaggtgact ataaatatat aaaagaaaaa ggaatagatg taataataga
     4201 aatagctaga ttttgggcaa gtagagttca tttgtctaca aaaaaagatt tgtatatgat
     4261 acatggagta acaggtccaa acgagtatga aaacaatatc aataataatt ggtatactaa
     4321 ttatatagct aaatggtgtt tagattatgc tgtagaaaat atcttacgat tagagaaaga
     4381 gtgtaatgaa tgtattgaca gaaataatgt gactagggaa gaagttgagt tttggaaaat
     4441 aatatcaaat aaaatgtatc taccgtatga tgaggaacta caaataataa ttcaaaatga
     4501 taatttttta gataaagaat ttgtagaagt aaaggattta ccaaaagaaa attttcctct
     4561 taatcaaaat tggtcttggg ataagattct taggtcgtgt tttataaaac aagcagatgt
     4621 attacaggga atttactatt ttggaaatag atttactaaa gaagaaaaaa agagaaattt
     4681 tgacttctat gaaaaatata ctgtacatga atcttcttta tcttctagta tttattctat
     4741 tattgcctca gaaatagata acctagaaaa ggcttatgaa ttatattcaa gaacagctag
     4801 gcttgattta gataattaca ataatgatac aaatgatgga ctgcacataa cttcaatgtc
     4861 tggttcatgg ctttccattg tacatggatt tgcaggcatg agaacatgga atgaaactct
     4921 atcttttgac cctaagttgc caaaagagtg gaaaaattat tctttcaata taaattatag
     4981 agataatatt attaatgtag atgtatgtag tgatttaatt aaaatagaaa atttaaaagg
     5041 tagaccaat
//
LOCUS       DAENCV010000202         4961 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAENCV010000202 DAENCV010000000
VERSION     DAENCV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.205"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            155..1348
                     /locus_tag="KQD82_003649"
     CDS             155..1348
                     /locus_tag="KQD82_003649"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG4382086.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1554..2672
                     /locus_tag="KQD82_003650"
     CDS             1554..2672
                     /locus_tag="KQD82_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG4382087.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYLKLLKYKEAID"
     gene            2803..3048
                     /locus_tag="KQD82_003651"
     CDS             2803..3048
                     /locus_tag="KQD82_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG4382088.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3035..3781
                     /locus_tag="KQD82_003652"
     CDS             3035..3781
                     /locus_tag="KQD82_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG4382089.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLAKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4064..4939
                     /gene="hslO"
                     /locus_tag="KQD82_003653"
     CDS             4064..4939
                     /gene="hslO"
                     /locus_tag="KQD82_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG4382090.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
BASE COUNT         1993 a          492 c          996 g         1480 t
ORIGIN      
        1 tatgcatatt aatttaataa aatataccaa aatggaaata aaaaataaat tttatgagaa
       61 tttaataact aataattaat atatttaggt tataataaat atgtgcgtaa aatctaacaa
      121 taagcaataa taccgtaaat ttaggaggaa taatatggat actttaaagg aactttttaa
      181 aataggaagt ggaccttcaa gttctcatac tatgggacca caaagagcag cagagagatt
      241 taagaatgaa aacccagatg cagaaagttt tagagcaata ttatatggta gtttggcagc
      301 tacagggaaa ggtcacttaa ctgactatat aattgaaaaa actatagcac ctaaaaaagt
      361 cgaaattgtt tgggaagaag atattataaa agattttcac ccaaatggga tgaagtttga
      421 agctttagat aaagataaaa atgtaacagc tgaatggact gtatattctg taggtggagg
      481 gactatagca gaagaaggtc aaagaaacag taaaagcaat agtatatatc acttagatac
      541 tatggatgag atagtaaaat ggtgcaaaga aaataataag actttggtag attttgtgtt
      601 ggaatgtgaa ccaaaagaca taaaagatta tataaaaact ataaaagatg ctatgagaaa
      661 atctattgat gatggactaa gtacagatga aataatacca ggcaagttat tattaaaaag
      721 aagagcaagt acattttata atgcatataa aaaagataag agcttctcaa cacttgtata
      781 tgcatatgca ctagcagctt cagagcaaaa cgcttctggt aatataatag ttacagcacc
      841 aacttgtggt tctgctggag taataccagg aatattcttt gcaatgcagg atttctataa
      901 ttatgatgac gaaaaaataa ttgaagcact acttgtagct ggaataatag gtaatataat
      961 aaaaactaac gcttcaatat ctggagcaga ggtagggtgt caaggtgaag ttggagccgc
     1021 ttgttctatg gcagcagcag cagtagcata cttaaaaggt ggaactatag accatattga
     1081 atatgcggca gaaatagcac ttgaacatca tttaggaatg acttgtgacc ctgtatatgg
     1141 atatgttcaa attccatgta tagaaagaaa tgcaatggca gctcaaagag cttatgatgc
     1201 agctaattat gcattactaa ctgatggctc acattctgtc tccttagacc aagttgttga
     1261 aacaatgaaa gaaactggta tagatatgat ggataagtac aaagaaacag ctaagggtgg
     1321 acttgctaaa catttcttct cttgctaaaa ataaaatttt ccaataataa ttcctctgtt
     1381 ttgatttatg aaacagggga attattatta atggtgaatt atttaataat gcaaaatatt
     1441 tttatattca atttagcttt ttatttttaa ttagtataac atgctatata atattttaat
     1501 tataaagaaa tatagaaata ttttttaata aattagaaat tggaggagat tacatgagag
     1561 aggcatcata tttacaagaa caactttata aacataggaa gtcattaaat cctattgcag
     1621 agattgggag aaaagagtat aagacttcaa aatatataag agattatctt gataaattag
     1681 gaattgagta tgaagtttat ttagaaacag gtatagtagg tgtaatagaa ggtaaaagac
     1741 ctaaaaaaga tattgctttt agagctgaca tagatggtct tcatagtgaa gaaggggtaa
     1801 aacatttatg tggtcatgat ggacatgcta ctatattact tggattaatt gagtatttaa
     1861 atgataataa agaagactta aatgataata tagtatttat attccaacca gcagaagaag
     1921 gtcctggggg agcaaaagct ttaatagatg aaggagtgct tagaaaatac aatattgatg
     1981 aaatatatgg gcttcatata tatccagagt taccagaagg ttatgtaggt acaagagagg
     2041 gctattttat ggctcaaata ggagatattg acatagaaat agtgtctaaa agtggacatg
     2101 gggctatgcc acaaaatggt attgatggta tagtgatagc ttcaaacttt gtaagctcac
     2161 ttcaaactat agtgtctaga aatataagcc caatagacaa tgcagtgttg actattggta
     2221 gaattgaagg gggagctaga agaaatataa tagctgaaaa tataaagatg gaaggtacta
     2281 tgagatgttt cagcccagaa gtgtatgaga aaatgaagct tagagttaga gaacttgcta
     2341 gagggtttga attggcttat aactgtaagg ttaatataaa tattatagat gattatattg
     2401 ctgttaaaaa tgacaaggat ttgtatcaag agtttgtaga ggctattgga agtgatactg
     2461 ttgtagagtt agagccactt atgatatcag aagatttttc ttattatcaa aaagaagttc
     2521 caggattatt ttttatgttg ggttcaagaa acgaagaaaa aggatttgta aatggtcttc
     2581 ataatataaa ctttaatttt gatgaaaaga tatgtgtaaa tgcattgaat gtttatttaa
     2641 aattattaaa atacaaagaa gcaatagatt aggaaaataa attataaaga ttaaaaagca
     2701 taaataccta aatttaaaaa aagtgtatta ataaataccc cttacatatt tgtataaatt
     2761 tattttaatt gacaaaaata caaataagaa ggggtgattt ttttgaaaga aaaagtacaa
     2821 agagaaaaga agataactaa tggcaagaat actaaaatat tgacaaatga tgatataatg
     2881 aaatatgaga tagcctcaga actaggtctt atggataaag ttggtgaacg tggatgggca
     2941 ggacttacag ctaaggaagc aggaagaatt ggaggtatgc ttacatcacg caaaaagagg
     3001 cttaaaaaga ttgtggagga gcagaataaa aaggatgagt caatatagtg attttgcgtt
     3061 tgtatatgat gaacttatga atgaagttga ttataatggt tgggttaaat atatagaaga
     3121 tataatagat agtgaaggtg taaaagtaaa aaatatattg gaattggcat gtggtacggg
     3181 taatcttaca attccattgg ctaaaaaaaa ttacgatata gctggtatag atatatctga
     3241 tgagatgctt agtgtagcaa gagaaaaggc agaaaaagaa ggagtagaat tagttttact
     3301 tcaacaagat atatcagagc ttgattttga aatatctgat ttggactgtg tactttgtgc
     3361 ttgtgatgga tttaattata taacttatga tgatgatttg gaaaatgtat tttcaaagac
     3421 atatgaatta ttaaagaaag atggaatatt catatttgat ataagttctt tttataaatt
     3481 agccaacata ttgggaaata acatgtatgg agaaaataga gaagatatag cgtatatgtg
     3541 gcaaaattac tttgatgaag aagagaatct aattgaaatg gaacttacat tctttattaa
     3601 agatgaatgt ggaaagtttg aaagatttga agagatacac caacaaagag catatactga
     3661 agatgagata gttgatatgc ttgaaaaatc tggatttaag gatataaaat attatgggga
     3721 ttttacattt gacaaaccta gtgaagaaag tcaaagaatc ttctttgtat gtaaaaaata
     3781 agatattctt gactatataa gataattatt aaaaataacc atgatataaa aatatagaaa
     3841 atgcagtttt atagattaaa agtaaagtga tgatacttta tgacttttat tttttaataa
     3901 ttataaagtt ctatagataa aaattaaaat atagtaacta ttacttagta aaattaaaat
     3961 aaaatgggtt ttgcccaagg ttaagataga taagtattta tagataaatt aaggtagtaa
     4021 tataataaaa ataagacaat taacgggaca tggaggaata ttaatgagag attatgtact
     4081 aagagcgaca tcaggaaatg gtcaagttag agcatttgtc gcaactacta gaaatacagt
     4141 agaagaagct agaagattac atgaaactac aaaagtagca acagcagcac ttggaagaac
     4201 attaacagcc acatctataa tgggattaat gatgaaaaat gatagtgata aattgactgt
     4261 aataataaaa ggtggaggtc caattggaac tattatcgcc acatcagact ctaagggaat
     4321 ggtaaaagga tatgtaggaa atccacaagt agaggttgaa gactatccaa atggaaagtt
     4381 aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa gttataaaag acttaggtct
     4441 tagagaacca tataatggga cttatccatt ggtaagtgga gaaattgcag aagactttac
     4501 ttattatttt gctgtatcag agcagactcc atcagtagta gctttaggtg ttcttacaaa
     4561 agaagatgaa gtagagtttg ctggaggatt tatagttcaa ttaatgccag atgcagaaga
     4621 agagactatt gctaagttag aggaaaatgt agctaaatta ccttctataa ctaacatgtt
     4681 aaaagaagga aaatcaccag aggatatatt aaatattgtt ttggatggtt tggaacctaa
     4741 aatacttgat acctgtgaag ttgggtttat gtgtgaatgt tcaaaagaaa gagtaaaaac
     4801 tgctttggtt gcaattggta aaaaatcatt ggctcaaata atagaagaag ataaaaaagc
     4861 agaagttggt tgccagttct gtaataagaa gtatatgtat agtgaagaag agttgcttga
     4921 aattttaaag gaaatgtaga ctataaaaat agttgtattg a
//
LOCUS       DAENCV010000203         4749 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.206, whole genome shotgun sequence.
ACCESSION   DAENCV010000203 DAENCV010000000
VERSION     DAENCV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.206"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            786..1448
                     /locus_tag="KQD82_003654"
     CDS             786..1448
                     /locus_tag="KQD82_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382091.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1457..3964
                     /locus_tag="KQD82_003655"
     CDS             1457..3964
                     /locus_tag="KQD82_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382092.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQIMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4295..4639
                     /locus_tag="KQD82_003656"
     CDS             4295..4639
                     /locus_tag="KQD82_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382093.1"
                     /translation="MYCLSVVLVIYGIYVFINCTEYIYTMLSTKQLTVTGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGSIENSILKK"
BASE COUNT         2093 a          419 c          701 g         1536 t
ORIGIN      
        1 atataaataa atattattat aatcgttatt aaagaccaaa aatcccatct tagaagtttg
       61 tatcatatga aacctttata gattatgaaa gcaataatat agtgtatgat atataaaata
      121 agttataaaa gtattttttt agatattcaa aaagacattg taagttatat taataatatg
      181 ggtatcaacg aattttggat ataatgagtt ttttaactta aagttatgta ataatgaatg
      241 taaagaatat ttaatgaaat cattttgttg gttatgtcta gctgaataca ataatctttt
      301 agataaagat tttatatttg aagataggtg tgaaaaaatt aagaagatat taacatcagc
      361 agtttagagg caaagcatgt aaagtaaagt ctgggtaact aaaagtagta atttagattg
      421 taactattgt tataaatatg aaaatgagga aacaggaaat ataagttagg atgtagaaaa
      481 taggttacta aataaaaagc ttaattatat aatcaaagaa ataggtatat actgtacaat
      541 ttcatggtga agaacttttc ctaaataaaa aggttggtaa aagagatata agaaataaaa
      601 ttcaattata agatatggta tgataactaa aggcactata tggagtgaat aggcggaaga
      661 attttttgaa aattataaaa atagtttctg ttgctacata tcaataagta tagatagaga
      721 ttgcaaaatc catagcaaaa atcgaattta taagtgtaaa taacatgtaa gacaaggagt
      781 aaattatgag tattttagag ataaaaaacc taaataaaat atatggtaaa tcggaaacag
      841 aggtgaaagc gttaagtcaa ataaatctaa aaataaatag tggagaattt gttgtgatag
      901 taggtaaaag tggaagtggg aaaagtactt tactacacat aatggcagga cttgaaactc
      961 caacaaatgg tgatgtgatt attgataata taaatatatc atctcttgat gaaaagaaaa
     1021 gatctgcatt acgtaaagaa aagttgggat taatatttca atcatataat ttgattccag
     1081 ttttaactgt tgaagaaaat ataaaactac caactataaa tataaaaaat aaagatgaag
     1141 cttatattaa tgaattgatg gagttactag gaataaaaga tagaagaatg tatttgccaa
     1201 atcaattatc aggtgggcaa caacaaagag tagcaatagc aagggctctt gtaaataaac
     1261 catcaattgt atttgctgat gaacctactg gaaatttaga tacaaagaca gagtcagatg
     1321 tattaagttt attaaaagaa tctcaaaaaa aatataatca gacaataata atgataacac
     1381 ataatataga tataacaaaa tatgctgata gagttataga aatagaagat ggatttataa
     1441 gagagtaggt agatttatga aacttaatta tattgtaaaa aaatatataa tgggaaataa
     1501 gaaaaataca ttattaataa ttgtaagtat tgtaatttca acagcgttgt ttttaattat
     1561 gaatataata agtgaagatg cgacaaactt aatgataaat caagctaaaa atgaatttac
     1621 attaaaacat gcacaatata tcaatccaaa agacgaagaa ataaaatata tagaaaataa
     1681 cacaagtata gataaagttg gaaaatcaat gcttttagga attagtgata ttggtaaagg
     1741 tcaaacacta caaattctat ctcaggataa agttgctgaa gaactaaatg atatttacac
     1801 actagaaaaa ggtaaattac cagtagctga aaatgaaatc gctatagatt catggtatat
     1861 taaacaaaaa aagataaaaa accctatagg aaaaaggata acattagatt atagaagaca
     1921 agggatagat caagaagatt tatatactgg ggaaaaagaa tttaaaataa ctggtatctt
     1981 aaatagtaat cctattttaa aagctcaagg aacatcaatt ggccttataa gtaacgattg
     2041 tgctatcaag aatattccta ctaaaaataa atatgatcaa attatgttta cttttaaaaa
     2101 ggaaaaaaat atacagagac aatcacaaaa acttattaaa aatggaaatt tgaatgagaa
     2161 taacatttat tttaataatg aattattaat agctatatct gatagtatga atttaaaaat
     2221 accttatata atagttaata tagtactagc actagcaact atattattaa tttataatat
     2281 tttttatata ttagtatcaa atagaataaa ggattttggg atatttaggg ctttagggtt
     2341 taatccaaat gatattttta aaataatgat tttagaagta tctatatact ctattataag
     2401 tatttctata ggtctaattt taggtgggat aatagctagt ttatcaaggg agtatgttat
     2461 aggagtaata tacaatgtca actatataaa ttctataaaa aaccagaact atataaacac
     2521 atacataatt tctatattat taagtttagg aactataata atatcagttt caaaacctct
     2581 tatgttatca gttaaaactg atccaatgat ttgtataaga agaaataatg aaaaagtgga
     2641 tataaaacaa aattccttta taaataaatt tatgataaaa cttttcaaag actatggtaa
     2701 catagcatca aaaaatatac aaagaaataa gaaacgtaca aatttatcta tagcatcaat
     2761 ggtaattata tttttcttaa tggcaacaat atacacaaag tctactagta actttttaag
     2821 tgatggtgta ttaaaatatt ggatacaagg agattattta ttacataata ttgatataag
     2881 tactatacgt tcaaataata aaggttatga taaaaatgta ctacaagaga taaaggcaat
     2941 tgatggtgta acaaaagtaa actcatatag gcataagtgg tttaatataa aaattgatga
     3001 taaaagtata aataagaact ctgattattg gaaaaaaaat aaagataata ttgaagctag
     3061 atcagaagta aaagatggaa ttaaggtata taataattct tttgaatttt taggaataga
     3121 agatacagat atactagagg atttcttgat aaatggaaag gaaaatatag ataaatttag
     3181 taaacaacca tatctgtata taacaaagaa atccagtgat tcgcttaata taaaaaaagg
     3241 agatagaata aaagtatact ttgatattat agattcaaaa acaaataatt ttaccaaaac
     3301 aatatctcaa gaatttatag tatgtggaat tataactaca ttaccagtaa cctcacaagt
     3361 gggagctgaa tttggcggag taatatcaac aaatcaattt aataagttta caggaatatc
     3421 atcttatgaa agatttgaca tatggacgag taaactagcc aatgataaat atgttgaaag
     3481 tgagttaaac aaaataacag aaaaaacaaa taaaggaatt ttaattccat acaaagctga
     3541 aacagctgaa tatgaaaaaa ttgataatca aaaaacacta attatggtat tagtaactgg
     3601 aattatagtt atattatctt tatttaactg ttgcaatact atagttacta gtattaatag
     3661 cagaagtagg gaatttgcac tatttagagg aataggaata tcaaaacatg aaataaataa
     3721 aattgttaaa ttggaaagtt atatatatat tatagtaagc ttttgtatat ctataatacc
     3781 tattttaatt gtaagaagta taataataaa accttttgaa agtatcaatt taataaattg
     3841 gaaatttata ggggctatta tcctaatagt atttatttta gtatctataa tcatgataac
     3901 tacactaaaa acattgaata aaattcaaaa tgaaaatttt atggaagaaa taaagacttt
     3961 atagtgaaca gcctataaat taaagaggtt ttatatgaat ttaattgcaa ttatcgataa
     4021 tggtgtagat attgatttta aataatttag aaataaaaat aaaataagtt atccaagtta
     4081 tatgtatgat gtaatagggg tacaatgtat agcaaatata aatattggct taataaaact
     4141 ctactcttaa gtctactgtc gttttaacac aagactaata ataagtgtaa cctaaaaatt
     4201 attgataata caataacctt gtttatgata aataacaaat attaagtgtt gtaaagatag
     4261 taagtttaaa aaaatatcaa agatttcaat cacaatgtat tgtttatctg tagtgttggt
     4321 tatttatggt atatatgtat ttataaattg tactgaatat atatatacta tgctatctac
     4381 aaaacagtta acagtcacag gaaatgaata taccattata aattattata tgagtaactt
     4441 tagtcagtat ataatatttg ctattttact tgcatcaatg ggatttgtaa tacaaaagat
     4501 agtgcatatg catttcagtg agatagaact tagtggtaac tattcaacaa ctgttagaaa
     4561 tataagtgat atctctttag ataatgataa tgaaatgttt aatagaggca gtattgaaaa
     4621 tagtatttta aaaaaataaa taatataaat atataaaatg tgattgaata aagcttagaa
     4681 tacttagtgt attttaagct ttttttatta ttaagatttc aaagtattaa attaactata
     4741 taaaatcat
//
LOCUS       DAENCV010000204         4737 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAENCV010000204 DAENCV010000000
VERSION     DAENCV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.207"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            101..1270
                     /locus_tag="KQD82_003657"
     CDS             101..1270
                     /locus_tag="KQD82_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG4382094.1"
                     /translation="MNKLCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1433..1975)
                     /locus_tag="KQD82_003658"
     CDS             complement(1433..1975)
                     /locus_tag="KQD82_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG4382095.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2123..2407)
                     /locus_tag="KQD82_003659"
     CDS             complement(2123..2407)
                     /locus_tag="KQD82_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG4382096.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2470..3234)
                     /locus_tag="KQD82_003660"
     CDS             complement(2470..3234)
                     /locus_tag="KQD82_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG4382097.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSITQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3516..3782)
                     /locus_tag="KQD82_003661"
     CDS             complement(3516..3782)
                     /locus_tag="KQD82_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG4382098.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(3824..4633)
                     /locus_tag="KQD82_003662"
     CDS             complement(3824..4633)
                     /locus_tag="KQD82_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382099.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1612 a          653 c          655 g         1817 t
ORIGIN      
        1 gggatagcct gtggagaatt agtaagacat attttagtat gcaaaattct gtgaagcagg
       61 aaccctgtga ctttagtcat gggaggttca gggtagaaag ataaataaac tttgtaaaga
      121 tgaagttgta gagttttgca atagaataaa aggtaaagtg gaaagtgttg caataagtgg
      181 agtatttgct ccagtaaata aacaacaaga agaagaagtt gcaaaatggg ttagagaaat
      241 tttaggggat attcctatat ctttatcaaa tgaaataggt aatataggta tattagaaag
      301 agaaaatgga gctattttaa attctgcatt gtctcaagta ggtaaagctg tatctttagg
      361 atttgaaaaa gcacttaaag acttaggtat agatgctcaa atatatttca gtcaaaatga
      421 tggaacatta atgaatttag agtatacaat gaaataccca atttttacaa ttggatgtgg
      481 aataacaaat tctataagag gagcttcctt cttatcaaaa gtaaaaaatg caatagtatt
      541 agatgttggt ggaactacaa gcgacttggg tgtactatat aatggattcc ctagagaatc
      601 atctattcca gttacaattg gaggagtaca tacaaacttt agaatgccag atgttttaac
      661 tattggtctt gctggtggta catgtgtaaa aggcgaaaaa gataatataa agataggtcc
      721 tgaaagtgtg ggatatagaa taactgaaga tgcaataata tttggtggag acactctaac
      781 tttatcagat gttgttacaa aattagatat ggcgtttgaa gatagaaaaa gcaatgttga
      841 aaacttagat aaagagtatt gtaatgaggt atataagaag gttattttaa aattagaaga
      901 tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa ttggttttaa ctggtggagg
      961 aagtataatt gttcctgaaa aactaaaagg cataagcaat gttataagac ctcctcatta
     1021 tactgctgca aatgctattg gtgcagcttt aggacaaata agtggagatg tagaaaaagt
     1081 atacagttta gataaaatgt cgagagaaga tgccatagaa gatgcaaaaa aagaggctgt
     1141 aaataaagct ctaaaagctg gtgctaaaga agatactata gaagtattaa atatagaaga
     1201 tgttccactt gcataccttc ctggtaatgc acttcttata aaagtaaaag ttgttggtga
     1261 tttaatttaa agaataaata taagtatttc cagaattata taaatatttc tggactcatt
     1321 tttaaactta ttttatcaat aaaaacagca taacttttga ctatattctt ataagtttcc
     1381 gaacagccga tatataccta ttttttgagg tttatcggct gtttgttatc ttttatcgca
     1441 ttccaactat ttttcttacc gtatttgctg tacgaattgt aatcttatca cttacatttg
     1501 aactggcagt ctttgaccac caatttgtct ttttataatc tttacgacta aaagaccaaa
     1561 agatagcatt tttataatga tatacttttt catactcttc tttaggattt ccaacctcat
     1621 cataaaactc gtcataagat aagtcaggaa acataaaaat aagattatca tatatttctt
     1681 tgttgttatc tccccaccaa ttaggagcat tatttagtat atcttttaac tcttcttgtg
     1741 aaataataaa aactggtatt tccaattcaa actgtttttt tatcattaat cttattgtat
     1801 ttgagataac cttgctgtca tctacatcac ttaaaaagac aacattacca ctattaaggt
     1861 atgtagaaac ttctgaataa ccaagttcta caaagcttgc ttttaattca gacataatta
     1921 tcttgttctt accacttata ttgatacctc ttaataatgc gatataactt ttcatataat
     1981 aatttcacct ctaaatattc acatttttct atgtagtaca aactggaatt tgcatctttt
     2041 ataattcttt caaaacttct tcatatgtaa tttttttaat ataaaaaaat cggtgcttaa
     2101 tttgtaccga tttttgtttt tcttagtaaa gaactcgttt ttttgcttct gctatagctc
     2161 tagcagcact ttttgcttgc attctttttt cttcttcttt tttaaattca tttattgttt
     2221 catttagatt cttatcatgt tcattttttt ctttatcttc gttttctctg tatactaata
     2281 tataatctat tattgattcg tttattatct gagatatact tttattttca ttaattccta
     2341 ttctcttaac ttccttaagt agagtctcat caatatgaac cgttgtttgc tttgtatttt
     2401 ttgccacctt aatcacctct taaaaaaaat tctaacatta aaattttttt tagtcaataa
     2461 attttaatat taaaattctt taaatgtttt atttgatttt ctaaaatttt ctattgtatt
     2521 tatcatctta ttttttatat tttcacctag tacagaagac tttagtctag gattttcgat
     2581 atggtcataa attggttcta aaaattcgac ttcgacagga acagagttaa ttttaccaat
     2641 aggttggtat ccttcataag tatcttttga attttttatt actagtggaa caatagggca
     2701 ttttgcttta tatgctatct ttaaagctcc acttctaaac tccgaaacca atgaatttgg
     2761 ttctttaatc catgttaaat ctccttctgg aaacactgcc atactttgac ctgtaagtat
     2821 attttgagag gcttgggtta tactctttat accctcacga ttgttttttc tgtttacata
     2881 tacacatctt agtaattttg cccattcttt aaatataggt atatttctcc aaactggttc
     2941 atctgcaatt acacatccaa ttggtctttc tacacttgct gttagtatga agctgtcaag
     3001 catactagaa tgatttacaa caaatagaag tggttctttt ggcagtgttt ccttaccaat
     3061 tatattaagc tctatgttca ctatatctaa agatttttta gcttgttttt gcataaattc
     3121 aaatttttcc tttaagctaa atttatcagg attcttttta attttaataa gcctaggtat
     3181 agaagatgta aaacctataa gttgatatgt agcaaatttt aagtaattat tcataaaatt
     3241 atctccttaa aataaaatta tttattactg taaagatttt aacataaaat aaaatttaag
     3301 tctattttaa ttcaattttg taacatatat tttaaatata cagagattga gattttatat
     3361 ttccaatttt agtaatcaga ttttatcaat aaattactat tctattaatt taattaaata
     3421 acctttaaat ttaattataa aaactgttgc agaaatttct acaacagttt aaaaaattta
     3481 ccttacaaaa acttatttta taacaagaga gtatattata aggctagtac agcccatttt
     3541 ttgcactgag aaattctttt gccatcatca ccttgttctt tgtcatatgc aaattgatta
     3601 agcatttcac atggaaatgt atcacataga ccacaatgtg taaggttttt atcttcacaa
     3661 caagatttta caggacattg ttcaccccaa aatggtttct caatctctgt acaaccttta
     3721 caattgactt tttccttgta tccacattca taacataaaa ttccacatct tgattctatc
     3781 attttaagtc actccttaaa tataaatttt atatgattgt atatcaaaat ttatactttt
     3841 cattgtaaaa atcggacaaa ttttttatat aggttgttgg attaacacca caaatagatt
     3901 taaaatcatt tataaaatga gattggtcgt aataaccaat atcttgtgct aaagttgcaa
     3961 atggtatttt attattttgt atttcttgta atacaaggtt tatacgaagt aatctaaggt
     4021 atgatttaac actcatacca agagaattat taaaaattct atttaaatgt cgttcactat
     4081 aacaactaat ttgtgaaatg tttttaacag acataataga gttttgtttt ctagcatttt
     4141 caagaattgg tatagtcata tctactatat ttgatttcaa taagtatgat aagaataaca
     4201 tatctaattg ttctactaat tcctttatag tacttgttct ttcaaaaatg gaattaacct
     4261 ctaagctaaa taatgtgtta aaatcttcaa gtgaaatttt taaatctgta gattctcttt
     4321 gagaaagacc tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat actctaaaaa
     4381 gtacattttc tatatcattt tttacaatag tagtttttgt tgtggctccc cagaatgcgc
     4441 tttctatacc ttttttgtca aatttaaata tcatacatcc actagcatca ggtattaatg
     4501 taagattttc tttaatactt gtatctggaa tagatattgt atagtgtgct atatactgtc
     4561 ttaacaattt atgtggcaaa atatatgtat actgtttgca ggtatttaaa atgttattga
     4621 ttattttatt cataagtata tcctcctgat atataaaaaa taagtatact aatttatttt
     4681 ttatataata aaccagtttt gttttaattg aaatattcta ataagatatt ttgttaa
//
LOCUS       DAENCV010000205         4733 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAENCV010000205 DAENCV010000000
VERSION     DAENCV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.208"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            154..1305
                     /locus_tag="KQD82_003663"
     CDS             154..1305
                     /locus_tag="KQD82_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG4382100.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1351..2526
                     /gene="thiI"
                     /locus_tag="KQD82_003664"
     CDS             1351..2526
                     /gene="thiI"
                     /locus_tag="KQD82_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG4382101.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2682..3485
                     /locus_tag="KQD82_003665"
     CDS             2682..3485
                     /locus_tag="KQD82_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG4382102.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3728..4369
                     /locus_tag="KQD82_003666"
     CDS             3728..4369
                     /locus_tag="KQD82_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG4382103.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1906 a          447 c          918 g         1462 t
ORIGIN      
        1 aaatttgtaa taaagtgaaa aaactagctt ttcatgaaat aatattttat aatttaagat
       61 tgtcttttta ataaagatta acaaaaaaaa tactataatt agtggtataa tagttagagt
      121 tgaagatttt aaataaaggt aggtgtaatt aagttggaaa tatacttaga caatagcgca
      181 actacaaaac cttatcaaga agttatagat aaaatggttt atgcacttaa tacggaatat
      241 ggaaatcctt cttctgtcca tagaaaaggt gtagaagttg aaaaagctat aaaagaagta
      301 agacaagata tagctaaatc tctaggagcg aaagaaaagg agatttattt tacatcaggt
      361 ggtacagagt gtaataatac tataataaga ggtattacta gtttaaataa aaaaagaaaa
      421 aatcacataa tatctactaa catagaacat ccttctgttt taaatacatt aaaagattta
      481 gaagaagatg gttttgaagt aacttattta gaggtcggaa aagatgggaa aataaatata
      541 gaggatttaa aaaatgcgat aaagtcaact acatgcttag taagcatgat gcatgttaac
      601 aatgaaatag gaactataca gccaataggt gaagttggaa agtatttgaa gggtttaaaa
      661 gaaaaaattt atttccatgt tgatgctatt caatcatatg gaaaaattaa ctttagacca
      721 tctaaataca atatagattt tatgagtgta agtgcacata aatttcatgg tccaaaggga
      781 attggattta tgtatataaa agaaaataat cgccttaagc ctatgttaac aggtggtggt
      841 caggaaattg gaattaggtc gggaacagaa aatgtacctg gaatatatgg tcttggagaa
      901 gctgttagaa tactaaacaa agatttagat gctgttattt cgaaagtaga taacttaaaa
      961 aatatattaa aaaatgaaat aattgataat atagaagata taaaaatcaa ttctccagaa
     1021 gatggagtat gtcatatatt aaatgtatca tttagaggga caaaaggaga agttttactt
     1081 cattacttag agcaaaaagg tatttatgta tcaactggct ctgcatgttc ttctaaaaag
     1141 aaaggtagtt acgtattaaa tgctataggt cttacaaatg aagaaataaa tggaacaata
     1201 agatttagtt tatctgatat gaatacagaa gaagaaatgt tagaagctgt gaaagttttg
     1261 aaagaatcaa tttgtgactt aaggtcaata atgaaaagaa aataaagaat actgtataaa
     1321 tagtgataat aaattagttt aagaggtgaa atggtgtata atatattaat agtaaagtat
     1381 ggagaaattg gagtaaaagg aaagaataga tatatatttg agaataggct tataagaaat
     1441 ataagaaata tgttaaaacc aattggaaga tttaatgtat ataaagagta tggaagaata
     1501 tatgttgatt tagaagacta tgattatgaa gaagtaattg aagaagttag aaaagtattt
     1561 gggatagtgg gtgtgtgccc tggagtaaga gctaaaaaag attatgatac attaaaagaa
     1621 atagctttaa aaatgttaga agaaaaaata gaggctggat ataaaacttt taaggtagag
     1681 tcaagaagag gagataagtc tttcggattg acttctcaag aaatgagtat ggatataggt
     1741 ggatacttat tatcaaaggt aggagatagg attaatgttg atgtaagaaa tccagaagtt
     1801 aaaataaagt gcgaatacag agaatttcat actatggtct atagtgatac tatacctgga
     1861 tatggtggat tgccacttgg aacgaatggt agagctatgt ctcttttatc aggaggtata
     1921 gatagcccag tagctacatg gatggttgca aaaagaggta tggaagtaga ggcagttcac
     1981 tttcatagtt atccatttac aagtgaaagg tcacaggaaa aagttaagga tttagctaaa
     2041 atactggcaa aatactgtgg tagagtaagg cttcataaag ttaatatctt agaaatacaa
     2101 aaagcaattg gcgagaattg taatgaagaa gaagctacta ttctttcaag aagatttatg
     2161 atgaggatag cacagagact ttctgaaaaa agacattgtg atgctttaat tacaggtgaa
     2221 agtattgggc aggttgcatc tcaaactata caaggactta cttgtactaa tgccgttgta
     2281 gacttaccag tgtttagacc acttatagca atggataaat cagatatagt tgatatagcc
     2341 aaaaaaatag ggacttttga aacatctata gtgcctgaag aagattgttg tagtgtattt
     2401 tctcctagaa agcccgtcac taaacctaga ttagagaaaa tagaaaaatc tgaaacagca
     2461 cttgacattg aaaaattagt acaggatgct attgacaaaa tagaggtaga ggatattgag
     2521 ttttagcata caagttattt aattattaaa ttgaatattg agtatattag tagttctgta
     2581 agtatattag tagttctgtt gaaaggattt atgtagacaa tttattaata tgtagtattt
     2641 aaaaataagt actataattt tatttaatgg gaatgattat tatgaataaa aagggtttta
     2701 cattaattga attgttggta gttatatcta taataggaat tttagttata gtagctgttc
     2761 cagcgttatt tagaaatata gaaaaaagta aagcagttac atgtctttct aatagagaaa
     2821 atataaagac tcaaattgtt attgcaatgg ctgaggaatc aagtaaagac aagaatgaag
     2881 tcataaaaga ggtattagaa aacaaagatg gtaagtactt tgaaacagaa ccaaagtgta
     2941 agtcaggtgg aatatattca gcaacgtttg atgatggtta tgatggaata actggaatag
     3001 aaagcattgc aaaagtgtat gttacttgta caaaacatcc agacggtgtt gaaatggcta
     3061 gggatgtgca tcaaagtatg aaagatttga ttgcatcatt tgcacaagac ccttctataa
     3121 taccaggagc ttcaaagggc aatgatgatt ttagaaaata tttattagac aataaatata
     3181 aaaatgggtg gcctacaatt ccagatgaat ttaaggcaaa atatggatta agtaaggata
     3241 cactatatat acaaccatat gcatataatc ctactaaatc tgatgctact gtagttgtat
     3301 ttgcaaataa taagactgga ggtaattggt atacttccct agtttacgat tatgatgaag
     3361 gtagatggta taaaggtaaa aatggtattt ctgttgcagg taggtcatgg gatgttgaca
     3421 cagatagtgt taagtctgta aaaacagaga ttcattctaa agagggatgg ggtcctttaa
     3481 attaatatat catgttttat taatctggta ttgattaaac tatagaaaag agcagtttct
     3541 atagatgacg aataccagat tttcatttat atatataatt gagatatatt aaaagtgttt
     3601 atatttttaa gtcttcacat acaagctaaa aataattata ataatattgt aaagaaattt
     3661 aattataaga ttaattttct gaagatttag tatttgtact aagaaataca taaaaagggg
     3721 aatagatatg aataataatt ttcaagacaa ccaaacaata aatttaatac aaagtcgtag
     3781 aagtatacga aaatttacga cagaacaaat tagtgatgag caagtaaaca cattattaca
     3841 ctgtgcattt gcagcacctt caggatgtaa taaacagcct tggcacataa ctgttgtgca
     3901 agaccagaaa cttttaaaag agattagtga tgatacttta tctaggattc atgaggtgag
     3961 taatgttgag ataaataaaa actttaagtt attttatggt gctccaaccg ttttatttat
     4021 ttcttatgat gaaagtagtt catgggctcc atatgatata ggaatactta cagggaatat
     4081 aacaacagct gcacaagcac tagggcttgg aagttgtata ataggaatgg ttagaggttt
     4141 atttacacct gttgaacaag gggatattga aggtcttgtg agtgtattgg ataaagaaga
     4201 tgtaaaagaa tcagagtcaa taaagatgaa gtttgataca aataaaaaat atagagaact
     4261 acttgatata ccagaagggt attctgtacc atttggtatt gctgtaggta ttccagatgg
     4321 aaatttacca aatgctagag aggtagttta taaagtttcc agagtttagg atgaagtatt
     4381 gcaagaaaca atcagttact tgaagaatta aatcagtaat atacaaataa attgatggaa
     4441 gaggaagcta tttaaaaagt agtttagatt tttcgttgta aaataattaa ataaagaata
     4501 aggtgataag tattttattt attatcttat tcttttttat ttttgttttg tagaatgata
     4561 aaaataagga ttaagtgtaa taaaatagaa aacaagtatg ttaattttaa gtatatgctt
     4621 taaaatttat ccaagttaaa aatttaagat atcttttcag tataatggaa aaaatgtcaa
     4681 aatattgaaa tagggaaaaa atagtgatat aataagaaaa aatagagaaa aaa
//
LOCUS       DAENCV010000206         4683 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAENCV010000206 DAENCV010000000
VERSION     DAENCV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.209"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            338..1813
                     /locus_tag="KQD82_003667"
     CDS             338..1813
                     /locus_tag="KQD82_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG4382104.1"
                     /translation="MDSEFANVVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1900..2313
                     /gene="speD"
                     /locus_tag="KQD82_003668"
     CDS             1900..2313
                     /gene="speD"
                     /locus_tag="KQD82_003668"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG4382105.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2343..3194
                     /gene="speE"
                     /locus_tag="KQD82_003669"
     CDS             2343..3194
                     /gene="speE"
                     /locus_tag="KQD82_003669"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG4382106.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3184..4062
                     /gene="speB"
                     /locus_tag="KQD82_003670"
     CDS             3184..4062
                     /gene="speB"
                     /locus_tag="KQD82_003670"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG4382107.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4242..4442)
                     /locus_tag="KQD82_003671"
     CDS             complement(4242..4442)
                     /locus_tag="KQD82_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG4382108.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1894 a          513 c          781 g         1495 t
ORIGIN      
        1 agtttagtaa taaaatactt ataaattaaa aatagtaaac tcaaaagctt actaaaactc
       61 aaaaaagtaa aaaaaccaaa ataataaaaa ataagaaagt ttataaatac taagttttaa
      121 aaaaattaat ataaacaaca aaaaattaaa aagtttataa atattgatat agctaatgta
      181 tatgtttttg agtaacttca aaaaaacact tgtaaaaatg ctgagatgtt aatataataa
      241 atatcatgag tttaataaaa gtaaaaataa aaaatagaaa taataaataa taaatattaa
      301 aaatatttga atatagagag gagatgaatt tgaaatcatg gatagcgagt ttgcaaatgt
      361 agttgaatta agctatgagt gtgctccatt attagagtcg ttaaaggaat actcagagaa
      421 agatatagct tgttttgatg tgcctggaca tgtaaaaaat agaggtgtag ctatattaaa
      481 taaatatcta ggtgaaagtc tcatgaaaat ggatataaat tcatcaccaa ctatggataa
      541 tgtatctgcg ccaaatggga ttataaaaaa tgcacaagat ttgttagcac aagcatatat
      601 ggctgatgag gcattcttta taacgaatgg cacaactcaa gcaatacatg caatgatttt
      661 gagtgttata aaaccaggtg aaaaagtatt acttccaaga aacatacata aatcagtaat
      721 aaatgcgtta atactttgtg gaggaatacc aatatttata caaccagaat ttgatgaaaa
      781 actaggtata agtttaaata ttactcttga aaaagttaga acagagatag aaaaagattg
      841 taatataaaa gcattatttt tccttaatcc aacttactat ggagtttgtg cagaccttga
      901 gagtattata gaactatgcc ataaaaataa tgttttagtg ttagttgatg aagctcatgg
      961 tgcacatttt ccatttcatt tagatttacc tccttctgca ataagtttgg gagctgatat
     1021 ggtagctgta agtatacata aaactggagg agcattgaca caatcttcag cactcttatt
     1081 gaatagagat aatgtaagtt ttgaaaaggt tcttcaatca atcaatatgc tacaatcaac
     1141 atcagcatct tatttgctta tggcaagtat agatggtgct agagttaatc tagttgagaa
     1201 tggtgaaaag caactgtcaa aagcattaaa tctatctagg tatgcaaaat ctaaattaaa
     1261 caaaattgat ggaataaaag ttttatctac agaaatattg aaacaaaaag gtgttaaatt
     1321 tatagatgaa acaaaattgt gtataaatgt aaaagaatta aatttgactg gatttgaagt
     1381 atacgattta ttatataaaa atttttcaat tcaagtagaa ttgggagatt catataatat
     1441 actggcactt gtgtccattg gaacaaataa atcagatata gatagattag taaaggcact
     1501 ttctataatt gcaaagattt atagaaaaga atcaacatta aatgaattta atatggttca
     1561 aataaatcca attattaaac ttaatccaag agaagctttt tatgcaccaa aggaaagtgt
     1621 agaaatcaat tcatgtatag atagaatttg tggagaatca ataatggcat atccaccagg
     1681 tataccgatt attgctccag gagaattgat aacagaagaa attatggagt atattatttt
     1741 cttaaaaaat agcaatgcgt atcttacaga tgtacaagat aaaaacttag atagaatact
     1801 agtaataaaa taaattactt tattttgcaa gggcaagtat ttgttaagta gagtttatca
     1861 aatagaactt attaaaagta taaaggagtt ggggttgaaa tgaaatttga aacactagga
     1921 agacatatac tagtagaata ctataattgt gatgaaaacg tacttaaaga cccacgtctt
     1981 atagaaaatt ttatgaatga ttcagcatta aatgcaaaag cgacaattgt ggattctgta
     2041 ttccaccatt ttaatccttg gggggtttca ggagctgtta taattgctga atctcatttg
     2101 acaatacata catggcctga atatggttat gcagcagcag atttttttac ttgtggtgat
     2161 atagacccat ggaaaagttt tgaattatta gagcaacttt taaagtctga aagaagcgag
     2221 tctacagaaa ttccaagagg cttaactaca aaaattaaaa aatattcaaa aaaagattta
     2281 ggtaagatta ctcataaacc agaagcagta taaaacatac aagagaaagt gaggattatt
     2341 atatggaact ttggtataca gaagaatgga cagaaaatgt aagattttct ataaaagtaa
     2401 ataaacatct atttgaagga aagtctcaat ttcaaaggat agatgtattt gatagtgatg
     2461 aatttggaaa atttctaaca atagatggat taatgatggt aacatataaa gatgaattta
     2521 tataccatga gatgataact catgttccta tggctacaaa tttaaacata aagaaagtat
     2581 tagtcatagg tggaggtgat ggaggtacag taagagaatt aagccgttat cctcaaatag
     2641 aaaagattga catggttgaa atagataaaa tggttgtaga tgtatctaag gagtatatgg
     2701 atatatgctc atgtaaatta gatgataaaa gagtgagttt atattttgaa gatggtgtta
     2761 attttgttaa gtgtgcacat gataaatcat atgacctgat aatagtagat tcaactgacc
     2821 caataggacc aggcgaaggg cttttctcaa ctgattttta taaggattgt tatagaatac
     2881 tgactgatga tggaatttta gtaaatcaaa gtgaaagtcc gtattttgac tttaatgcta
     2941 aagagatgaa aagagctaat aaaaaattaa aacagatatt tccaatatca gaagtttatc
     3001 aagcacatat accaacatat ccatctggtc actggttgtt tggatttgct tcaaaaaaac
     3061 ttaatccagt aaaaaatcaa gatagaaatg gatgggagaa attaagttta aaaactaaat
     3121 attataatag tgatatacat ttaggttcgt ttatgttacc tcaatacgtg aaggagatgc
     3181 tagatgaaga ataatttcta tcatatgaac acttttatga gtatggacaa aaattatgaa
     3241 gaatctaatc ttatagtatt tggtgttgga tttgatggaa ctacttctaa cagacctggg
     3301 gctagatttg caagtagctc tatgagaaaa gaattttacg gtcttgagac atatagtcct
     3361 tttttagatt tggatttaga ggattataat atatgtgatt atggagattt agaaattagt
     3421 gttggaagta cagaacaagt cttaaaagaa atctatcaag agacatataa gattgttaga
     3481 gattcaaagg taccctttat gattggagga gagcatttag ttacattacc agcctttaaa
     3541 gcagtacatg aaaagtacaa tgatatatat gtaattcatt ttgatgccca tactgatttg
     3601 agggaagaat ataataatag taaaaattct catgcaacag taattaaaag aatatgggat
     3661 attgtaggtg ataataaaat atttcaattt ggtataaggt ctgggacaaa agaagaattt
     3721 aaatttgcta cagaagaaaa acacacatac atggaaatag gaggaataga tacgtttgaa
     3781 aatatagtta atatgctaaa tggaaagaat atttatctaa ctatagattt agatgtattg
     3841 gatgcatctg tgtttccagg aacaggtaca ccagaacctg gtggtgtaaa ttatagagag
     3901 tttcaagaga tttttaagat tataaaaaac tctaatataa atatagttgg ttgtgacatt
     3961 gtagagttaa gccctgatta cgatacaaca ggtgtatcga cagttatagc ttgtaaaatc
     4021 ttaagagagt tatgcttaat aatatctgat aaaattaaat agagtatttt atataaatag
     4081 tatatattat aatataagaa gttattttaa aaaagttagt ttaagtttga cgcttttcta
     4141 aagtaacttc ttatttttgt aattaaaaat aaatataata aacaacaaaa aagagccctc
     4201 gatagctcct tttctaaaat atatatattt aaattaaata attatattct atttacgttt
     4261 tcagcttgag gtcctctatt accttgagtt atatcaaagc taaccttttc gccttcttct
     4321 aaagttttgt atccatcatt ttgtatagct gagaaatgta cgaaaacatc atcttctccc
     4381 tctacagata tgaacccaaa tcctttttca ttgttaaacc attttactat tccgtttttc
     4441 attataagaa aacctccgca ttattaaaaa atacaaacta aaaaaataag tctgttctat
     4501 taatataaca taaattttaa ataaaagcaa ataaaatttt taaaaaaatt aattactttt
     4561 agaatttaat aaaaattgtt aaattataca gtgataagta ttgtaaaatt tgacgaaaat
     4621 gtaaatgtag ataataaatt taatatcaaa aaatgtaaca aaaaggtatg taagtaaaaa
     4681 gca
//
LOCUS       DAENCV010000207         4671 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAENCV010000207 DAENCV010000000
VERSION     DAENCV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.210"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            110..3151
                     /locus_tag="KQD82_003672"
     CDS             110..3151
                     /locus_tag="KQD82_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG4382109.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNVFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(3312..4586)
                     /locus_tag="KQD82_003673"
     CDS             complement(3312..4586)
                     /locus_tag="KQD82_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG4382110.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSINDK"
BASE COUNT         1714 a          669 c          704 g         1584 t
ORIGIN      
        1 aaagctatac ttataaaagc tttttttatt gataataatc tgttgattaa aataattaag
       61 aatgttatgc taattataaa ttattaaaaa gggaaagagg taatgtaata tgtttaagca
      121 aaggctctct aaattattat ctagtacatt agtattgagt atgttattta ctgctgctcc
      181 aaatattact tttgctgata atacaaaaga taattccgaa aaatatcaaa gttctgatat
      241 agaattacat gattatagta aaaacgcgga atcttacaca aaaactaaag ctcttgctaa
      301 agagaaaatc caaactttgc tatcaaagta tggtgctgta agtgctcaat atgctttgat
      361 tgataatggc aaaattgaga tttctggtaa tggtggagtt tacagcaaac aggataacaa
      421 aaacttaaat aaggataata tgtatagtat cgcatctatc agtaaaatgt ttactacaac
      481 agctgttatg aagttggttg atgatggaaa attaaattta gatactcctg ttgtaaagta
      541 tatacctgaa tttaaaatgg ctgatgatag atacaaggaa atcactccaa gaatgttatt
      601 aaatcattca tcagggttga tgggaagtag ttttaaaaac actattttat tagcagacaa
      661 tgattcttat ggtcatgata atttcttaaa agaattacaa aaacaaagat taaaagctaa
      721 accaggagct ttttccgttt attgtaatga tggatttact ttagctgaaa ttttagtaga
      781 aagagtatct ggtatgtcat ttactaattt tcttgataaa tacataaata atccacttaa
      841 cttacaaaat acaaaaacta cagaaaatag ctttgatagt tcaaaactag caaaagcata
      901 cgtaccttat tgggaagatg cagttccaca agataacctt aatgctatag gtgcaggtgg
      961 tttatattca agtgcagaaa atctatgtac atttgcacaa acatttatga aaaattcaaa
     1021 tggtattctt tctcctgctt cagtaaaagc tatggaaaac aaagaatatt taaatggtct
     1081 atggcctgaa ggagaagact ctatacttgg atatggttta gggtgggatt gtgtcaacac
     1141 ttatccattt aatcagtata atttaaaagc tcttacaaaa ggtggagatt ctttattatt
     1201 ccacagtaat cttatagttc ttccagatga aaatatggca gttgcagttc tttcttctgg
     1261 tggaagtagt caacttaatg aaataattgg acaagaaatc ttactatctg cattaaaaga
     1321 aaaaggtaaa attaaagaaa taaaacctga taaaacattt agtaagccac aacaagttaa
     1381 aatgccaagc tctttaaaag aaaatagtgg tctttatgct tcttcaaata tgataaaagt
     1441 agatgttaat gataatggta ctttaacagt atcttctcct tatatagaaa atggacctga
     1501 agataaatac gtatacatag gtcaagatag atttgtatct gaaaaaggta atagttgttt
     1561 aaaatttgta aaagaaaaaa ataatatcac atatttaaat atgagctctt atgatgatgt
     1621 ccctggatta ggtcagacag caagtttata ctatgtagct caaaaagtag atgacaacaa
     1681 tatatcaaat agtgtcaaag aagtgtggaa aaaaagaagt ggcaaaggtt actatctagt
     1741 ggatgaaaaa tatacttctc aaagctatat gtttggctct gtaaaagcaa gttttagtct
     1801 ttctgatgaa actccaggtt atatagttaa cactaaaata atggatgaga ataactcaaa
     1861 tgcttttata gaaatacctg gtgtaatagg tcgtgattta tccgacatta aacttcataa
     1921 agaaaatggt acagagtatt tatcttttgg aacactaacg tatgttagtg aagattctat
     1981 aactaatttg ccagcagaaa aatcttttac ttgtgaacta gaatccaatg gttatgcaaa
     2041 atggtataag attggtgacg atatcgcaaa taaaaaaata gaagttaatc ttcctcaaaa
     2101 ttcagctttt gctgtttatg atgataaagg tgttcctgta aactattctt tagttacaaa
     2161 gaacaataga gttagattgc caaaaggagg cgtaattgtt ttcttaggaa gtccaaatgc
     2221 tagatttgag gttacatatc aagatgaagt aaatgcaagt gcacttacag gaactgatag
     2281 atatgaaact tctattaaga taagtcaagc aggttgggaa aatgctgaaa atgcagtttt
     2341 aataaacgat tcagcaattg cagatgcttt ggctgcaaca ccatttgcat acaagaaaaa
     2401 cgctcctata ttattgactg gaagctctca aataaatgaa aaaactttgg ctgaattaaa
     2461 gagattaaaa gttaaaaatg tatatgtagt tggtggagaa gcttctataa atgaaaaatc
     2521 tttagatact ataaaatcaa acaatatctc tgtatctaga atttcaggaa gtgatagata
     2581 tcaaacttct atgaacattg ctaaagaatt gaacaatatt agcaatatat caaagatttc
     2641 agtagttaat ggcgaaaaag gattagccga tgcagtaagc attggagctg tatcagctca
     2701 aaatgatatg cctataatac ttactaatga aaatagtaat ataactgaaa taaataacgt
     2761 atttaaaaac aaaaagattg ataaatctta tgtaatcggt ggagaatata cagtttctaa
     2821 aaatatagaa agtaaacttc aaaatccaca gagaatatca ggtagcacta gaaatgaaac
     2881 taatgcaaaa gtaataaaag aattttacaa ggattctaag atagacaatt tatatgttgc
     2941 taaaaatgga atgaataaac aagatgattt aatagacgga ttatctgttg gagttttggc
     3001 tggaaaaact aaatctcctg taatgctagt aggaaactca ctagattata atcaaaaaga
     3061 actatttaaa actatgagat ttaaatctgt aacacaaatt ggtggaaatg gaaatgaaaa
     3121 ctcatttaag caaataaaag aaatagctta acataactaa aaaaggctgt attcaagtaa
     3181 tattaattga atatagcctt tttttattta agttataagt tttatatgtg taaaaattaa
     3241 atttaaatat taaatgataa gtttattaag atatagataa gtctaaaact ttgccttttt
     3301 taagttagtt tctatttatc attaatactt aacaatttat ctaaatatac tgactttaat
     3361 tgtttactgg ctaaaatctt tttagctggt tcaagcttta atatagctga cagtatagct
     3421 cccatagctc tatgactagc cagagcatta ttatcaaaaa ttaaattttg taactgccct
     3481 ttttcaattg ccataagaac tgctctatgt actgaatttg ctggagttat tattttttca
     3541 tctctcttta ataacataat gctattttta tggcaatttc taacacaaac tccacaacct
     3601 agacatctat cctcatcaat ttttatatat tctttaccat cttccttaac cttgcttatc
     3661 gcatctattg gacatgcagt tatacattta ccacacttta cacaattttc atggtttata
     3721 tttggtataa aacttgttgt ctgtactgga tgaagatttc caaatctttt agctgctacc
     3781 attgcttcac agcaacatcc acagcaatta catataaacg taaccccttt cctaacattt
     3841 tctccacatt gaactaagtt atgttcatat gcttgatgaa gtagttcttt acattctatt
     3901 ttatccacac gtcttgcaaa tttattattt attaaagaat ttgctacatt gtcaaacgtc
     3961 atacatatat ccatgggagc atcacaagct tttccaacat gttgcattct atgtctacag
     4021 taacacatac ttatacctat atgagttgat tcatcaatta tatgagtagc cctttcataa
     4081 tctaaaatac taacctcatt atcattagtt aaaacttcct cttgaacgta tactcttcca
     4141 agcttcgttt ctgttgaata aaataaatct ttgataaaat cttcttctac attcatatat
     4201 tgatagtaaa gctctgccaa aagtttttgg tctatatcgt gtctcgttct catcatggca
     4261 aactcaaaaa aaccagccat tggaggaggc attatatatt tcttcccttt attatcttct
     4321 atatctagta tcagagcctt actagcaagt ttatccaata ctctatatgc ttcactttca
     4381 cttacactcc aaattttagc tgctgttttt actctgaaag gctttattgg aagctgagca
     4441 actaacttcg cctcttgttc tgtatatagt acatttagta ttttgtataa tgtatcagat
     4501 ggtggagcac cttgtggaaa cttgtttatt ctttcctcta aacttttata catattcttc
     4561 gcagttatat gagaaatagt aatcaccttc tttttctttt gttttccaat aaaatcaaat
     4621 attctttctt attatatatt ctcttacttt atataatacc aattctttct a
//
LOCUS       DAENCV010000208         4668 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAENCV010000208 DAENCV010000000
VERSION     DAENCV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.211"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(147..950)
                     /gene="proC"
                     /locus_tag="KQD82_003674"
     CDS             complement(147..950)
                     /gene="proC"
                     /locus_tag="KQD82_003674"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG4382111.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1275..3644)
                     /locus_tag="KQD82_003675"
     CDS             complement(1275..3644)
                     /locus_tag="KQD82_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG4382112.1"
                     /translation="MSRGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3649..4557)
                     /locus_tag="KQD82_003676"
     CDS             complement(3649..4557)
                     /locus_tag="KQD82_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG4382113.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1374 a          907 c          512 g         1875 t
ORIGIN      
        1 aagtaataaa ttctcactca atcattaaat tcctttttat agtaaattac tcttatttta
       61 cttataccaa atacatttat gtatagatat aaataagcac taaaaaaaca attcaagaaa
      121 ccatatagta aacttaatta catctgttat ttgctcatat ctttagactt atctatacaa
      181 gcaacaatag attttataac tgctgctcta aagccttctt cttctaatac cttaacagct
      241 tctatagtag ttcctgctgg agaacaaacc atatctttta attcacctgg atgttttcca
      301 gtttcaagaa ccatcttagc tgaccccatt actgcttgag atgcaaattt ataagcttgt
      361 tgtcttggca ttccagctaa tactgctgca tctgccatag cttctataaa cataaataca
      421 tatgcaggag aagaaccact aatacctgta actgtatcca ttaaatattc tgatactatt
      481 tctgcctcac caaaagattc aaacagagcc ttaatcatat ttaactcttc atctgttaca
      541 ttactatttt tacaaagagc tgacattcct tcgccaacaa gggctggagt attaggcatt
      601 actcttacta tttttttatc tgaacctata aaactttcca gactttctat agttttacct
      661 gctgctatag atactattat tttatttgca tctattaatt ctttaagtgg agttaaagcc
      721 ttttcgcata tattaggttt aagtgctact attaccacat cagaatttgt aacagctacc
      781 tttgaatcag ttgtagtatt tactccaaat tcttctttta ctttatctaa ggcaacttgg
      841 cttaaatctg atacagttat atcttctgcc tttactaatc ctgactgtac tatgccacct
      901 atcattgcac ttcccatatt ccctgaacca ataaatccta aagttttcat atcaaatctc
      961 cttttttcta ttagttttat tttaaagtag ctaaattgac tttattttaa ctgcttaaaa
     1021 ttatcttatt tttataaaat ataaccttat tataactata ataaattatt cttttgtatg
     1081 tattacttac taatatgtgt atgatttact aaatataaat ttaacgattt tttcattaaa
     1141 cttaacgagc taacttccta tttagaaagt tagctcataa ttaatctaaa attttaaaaa
     1201 tggaatcaaa ccatttatac tttattatat cagttacgat tttatatagt aagaagtttt
     1261 atttacaagc aaacctagaa tgtttgctca gttcttccta ttatctcatc ttgtagtgtt
     1321 ctacttaaat tattgaaatg gtcactatat cctgcaactc taactattaa gtctttataa
     1381 tcctgaggat tcttttgagc ctctaacagt acatttttat caaatacatt aaattgtata
     1441 tgatgaccat ccatattaaa gtatgctctt actagattag ccatattatc taaccctttt
     1501 tctccttgaa ctactgatgg tgcaaatctt tgatttaata atgttccacc agtttttatg
     1561 tggtccatct tagcacaaga ttttataact gctgtaggtc cattagtatc tccacctttt
     1621 tcaggtgaaa ttccctctga aactggtttt gcacaaagtc taccatttgg acttgctccc
     1681 ataatttctc caaagtatat atgacaagtt gttggtaaca tatctactct gtattctcca
     1741 ccacaaacag ttggtctacc agttacctca ttgtaatatg cattgaatat ctcttgcatt
     1801 atttcatcag cataatcatc atcatttcca tatttaggag tcttatttga aactaaatta
     1861 agtatagctt catatccttc aaaattagca tcaagtgctt taagcaatgt atccatatcg
     1921 aattttttct tatcaaatac attatatttt attgctgtta aactatctgt tatagttcct
     1981 ataccaacac cttgtatata tctagtgttg tatcttgctc cacctctttg gtaatccttg
     2041 cctttttcta tacaatcatc aactattgta gacataagtg gacaaggcat gtgttttgca
     2101 catatatttt ctatcacagc atttccttct atttttatgt caatcatata atgtaattgc
     2161 tttttaaatg cttcaaatag ttcctcataa gattggaaat ttctagggtc tccagtttca
     2221 atacctattt gttttttact aattgggtca tatccattat ttaatgttaa ttcaagtatt
     2281 tttggaatgt tcatatatcc agttagaaca tatgcttctt taccaaaaca tccagtttca
     2341 acacaaccac tggttccgcc aagtcttgca tcttctatag tttttccagc ttccataagc
     2401 tcttgaacta ttgcttcagt attataaaat gctggttgac cccatccttt tcttgatatt
     2461 tcacaagctc tctttaagaa tttttgagga gtctttttac ttatttgaac atttgaacta
     2521 ggttgtatca atttcatttc atccataaca tcaagtatta tataactaac ttcattaaca
     2581 ccatcttgac catctggatt tattccacca gtgtttatat ttgcaaaatc tgtatatgtg
     2641 ctactttctt ttaatgttat accaactttt ggtggtgctg gctgattgtt aaatttaacc
     2701 cataaacatt caagtaattc ttgtgctcta tctctatcta gtattccatt ttctacatct
     2761 ctttcataga aaggatttag atgttggtca agtcttcctg gagtaaaagc atcccatata
     2821 ttaagttcag tagtaacccc tatatgaaca aaccaataca tttgtatagc ttgatgataa
     2881 gtttctggtt tgtgagctgg tacaacatca caagtttcag caattagtaa taaatctttt
     2941 ttcttagctt catctgtttc ttcttctgcc atttgtcttg ctttctcagc atatctttta
     3001 cctaatataa ccattgcatc acaagctatt gccatagctt ctaaatctgc ttttttatta
     3061 tatgcatcta aatcattaag aaaatctagc tcttttaatc ttgcttctat gtctttcttt
     3121 aaatctaaaa atcctttttt atatattgta tctccacaaa ctgtatgtcc tggagctctt
     3181 tgttccataa actctgtaaa catacctgct tcatatgctg ctaaccactc aggtgtcatt
     3241 gcatttatta tcttatctct tgtttgtctc tttttccaaa aaggaattat ttcttcttta
     3301 tgtatttttc tagcttcttc tgatacgcta aaatttatta tatctctatt gtgcattact
     3361 tctaaatctt ccattgtatg acaacaaatt tctggatatg ttggtgctcc attaggagaa
     3421 tcaccttttt cacctactat taattctcca tcatttatgc taagagttct attctcaata
     3481 taatgcttaa atgataaagc acgaagtaca ggaatctcta cacttccctc atatttttta
     3541 taagcttctg tcataagtac tgctctttca atagatatgt gtggttctgc attaatactt
     3601 tcttctctta attttttagt tctctcaaaa gttcctcttg acattttatt accctccaat
     3661 cttagtattg aaattacttg cttcaaacag tctttttatt tcctctaatt tttcttctga
     3721 tggtctttgt agttcttctc cttcatactt tttataaatt ttatcatact tgtgtttacc
     3781 aatattatga tatggaagaa gacttactgc ttgtatattc aaaggcttta aaaactctat
     3841 cattttttta acctctaaat tttcatcatc tacatttact cctacaatta atggtattct
     3901 tatatttatg ttagccccta attcacttaa aatcttaaca tttttcagta ttaagtcatt
     3961 tgattttcct gtaaatttta tgtgcttatc ttcatcaata agcttaatat catataaaaa
     4021 caaatctgca catttagcaa ctctttcata attttctgac tttgcatagc cacatgtatc
     4081 aattgctaca tggattccct ttgatttgca catgtttatt actccacaaa tgaagtccat
     4141 atcttgagcc attacttctc cacctgatag agttactcca ccacctgatt cttcatagaa
     4201 aattctatcc ttctctatct ctttaaccaa atctcttaca gaatactctt gaccaacaat
     4261 ctctcttgca ttgtttacac aatagtctaa gcaagtttca caaaactcac acttatcttg
     4321 gtctaagcat atttttgttt cacctttgta aattgcctta tgagggcata tatttataca
     4381 tgcttcacat tttgaacatc tttcttcatt atacaaaact tgttttgtat atgtttgact
     4441 ctctggatta tgacaccaaa cacattcaag tggacaccct ttaaaaaata ctgtacttct
     4501 tatccctggt ccatcatgta tgctacattt ttgtaagttt ataactaatg gattcatgat
     4561 ttttcacccc taaataatat ccattccatt ttttcatttt tatgcaattc atcttcatag
     4621 aatataccta tgaaaagcat ttctacaatt acataatact atatttgt
//
LOCUS       DAENCV010000209         4643 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAENCV010000209 DAENCV010000000
VERSION     DAENCV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.212"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            610..1917
                     /locus_tag="KQD82_003677"
     CDS             610..1917
                     /locus_tag="KQD82_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382114.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2003..2265
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2391..3194
                     /locus_tag="KQD82_003678"
     CDS             2391..3194
                     /locus_tag="KQD82_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4382115.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPNNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3232..3843
                     /locus_tag="KQD82_003679"
     CDS             3232..3843
                     /locus_tag="KQD82_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG4382116.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3875..4546
                     /locus_tag="KQD82_003680"
     CDS             3875..4546
                     /locus_tag="KQD82_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG4382117.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1874 a          423 c          828 g         1518 t
ORIGIN      
        1 acaattattt ataactaaga taataaaaca acaatggtgc taatttatat gtataaatta
       61 attatgaaaa atttatataa gtaaaatata ttaacataaa ggtaaaattt tgtatactaa
      121 aagggaatat ttacagttta atggcagttt taaaataata aattaaagct gtcattttta
      181 tttaaaaatt tttagaaaaa gtacaatagt gttaatgtgt gaattagata aggtgtatat
      241 aatagtttag atgatatgat ataaaatatg ttgtaaatta aatgttttct ttgacaatat
      301 atacacaagg atgataatat ctatatgaat aaaagatagt gtgtaatttg ataatatgta
      361 attataacat tatgatataa ttacaataaa gtatgtaata atctgtagaa atttcaagct
      421 agttatctaa gtcggataac taaatctaaa cggaatacat aatgtattaa ataaatgatg
      481 atactaaaat gatactattt ttatagaaat ttattgaggt atataattaa tgaaaataat
      541 atattaaatt atataaatta aattatataa tatattgagt tttacataaa agcaaaggtg
      601 ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag ctatggtatc
      661 aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa aaaatataga
      721 tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt taaagcagac
      781 ataggttgtg ttgaagagat agacaatgaa gatgacaatt tagctttaga ttatgataat
      841 gctattcttg aagttcatgg atataaaatt atttctataa caataaatac tttaagtgaa
      901 tttagtgtag aaggttatat caatgatgac tgcataaaat ctaagaactc ctatgttgaa
      961 tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactcat taaatacaat
     1021 gataaggtat acgcaggtag aattgaattt gatataaatg attatgttgt aactatagac
     1081 aagcgatatg attatagaaa agaattaaaa tcagaattaa agtctaaatc tggagcaata
     1141 ataactcata taggtaggat tagaagaaaa gatggtagaa tttttagaac aaataataca
     1201 ataaatcttt tagataggat atcaacagct ttgagtttta tgtgtggaag atatgtaggg
     1261 ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg gaatgaaaat
     1321 caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa ttatcataat
     1381 atggaaaaat atataagcct tatgtgtaaa aagctagaag atttttatta tggttctgct
     1441 ataaagagtg ttgttgattg gtacatagaa tctcttggaa gtgctaccat ggaaaacaat
     1501 ataatatctg tacaaattgc acttgaaact ttgtcatatg taattctagt ggaacaaaat
     1561 aagatactta cagatgaagt atttgactgt aatttggcat ctaaaaatat aagactctta
     1621 cttgatacat gtaagatacc atatggtaaa catgaattaa atatatttga taatataata
     1681 aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag catagttcat
     1741 ccaagtagaa agactcatag agctgtgtta gaggttgaag atatttggaa tataatttca
     1801 ataggaacga gatatattga acttgtttta ctctttattt taggttatag aggagaatat
     1861 tcaaacagat tagtagaaag atgttatggt gaagtggaag ttgtaccttg gaactagatt
     1921 tttatattta ttttaaaata tatattgaca tatattgcct tatgctataa aatcaatatc
     1981 ataataataa ttcagaataa cattaaatgc taagaaaaga aatagtaaaa gtagttaatc
     2041 ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga acacatcttt
     2101 gagcaggact ctgaaagagt agaaatactt tattaggaga tttccggaat ccttgcaccc
     2161 gttatagtgc tagagtataa taattttatt gtacttaaag aggttagtgt cgtgaggcat
     2221 taatgaacag aggtggaacc acgagagtta actcccgtcc tctagcagaa gctagaggcg
     2281 ggagtttttt aatataattt tttaagcgcg cagagaatta tattgcaata gatacacttt
     2341 ttaaaaaata atcaaaatat tttaaaaatt aaaagacgag ggagaatatt atgaaaaata
     2401 tattaaaaaa agtaggaata tttacaatta tgttaggttt attaggtgga gttgtaggtt
     2461 gttcaaaacc aaataatgaa aaagataagg atgcttcaaa ggaatctaaa aaagaagtag
     2521 ttgtagggtt tgataatacg tttgtaccaa tgggattttt agatgaaaag ggagatacag
     2581 taggatttga cgtagactta gctaaagaaa catttaaaag acttggaatg gaagttaagt
     2641 ttcagcctat agattggtca atgaaggaaa ctgaattaaa tgattcaaaa actgtagatg
     2701 tattatggaa tggatattct ataacagacg aaagaaagaa aatagtatca tatacagaac
     2761 catatttaca aaataaacag ataatagtta ctctaagtga ctcaaaaata aattcaaaag
     2821 ctgatttaaa agataaagaa gttggaacac aacaaggttc aacagcactt gatgcagtgg
     2881 aaaaggataa agattttatg aatagtttaa aaggtggagc accagttctt tatgatactt
     2941 atgataaagc acttagagat ttagagatag gtagaactag tgcagttgtt ggtgatgaag
     3001 ttttaattag atactatatg ggacaaaagg gtgaagacaa atacaaggta ttaaaagacg
     3061 attttggatt agaagattat gttgtagcaa cttctaaaga aaatccagaa ctttgtgaga
     3121 aaataaatga aacattaaaa gaaatgaaaa aagatggaac ttttgacaaa atttatgata
     3181 agtggtttaa atagaagata agttagcagt taagaagggg gcactaaagg catgttacaa
     3241 ggtttagaga tagttatagc aatgttttgt ataacattga tagtatcaat tccattgggg
     3301 attggagttg catttttaag attatcaaag aataaattag taagtggtat tacacaatgt
     3361 tatatattga taatgagagg tacaccttta cttttgcaaa tgatagtcat attttacgga
     3421 ttacctcttt taggaattgt atttgacaga tttacagctg gagtagtagc tttcttctta
     3481 aattatgcag catattttgc agaaatattt agaggtggaa tacagtcaat cgatagagga
     3541 caatatgaag cttcaaaagt actaggattt gataagttta ctatgtataa gagagtaatc
     3601 tttccacaag tttttaaaag aatactagca cctatttcaa atgaagtaat cacattagta
     3661 aaagatacat ctttagtata tatattagga ttaaatgata ttttaagaat atctcaaata
     3721 gcaatgaaca gggaagcaag tctgttacca ttatttgagg caggagcgat atatcttata
     3781 tttgtagcta tacttactaa gggatttgaa ttacttgaaa aaaaatattc ttattataga
     3841 taaagttaca atattatagg aggaggtttt ttatatgttg aaaatcaaaa acttaaataa
     3901 atcatttaaa aaaaatagag tacttaaaga tatttctttt gagttagaag aaggtcagat
     3961 tggagtttta cttggtaagt ctggtgcagg aaagaccaca atattaaggt gtataaatgg
     4021 tcttgaagaa tttgatagtg gagagattat aatagataat gaggtaataa aaaataagag
     4081 agatatggca aaaatacgag gaaaaatagg tatggttttt caaaatttca acttatttcc
     4141 tcatatgaca gtccttgaaa atataattga atccccagtt aatgttttta aagttcctag
     4201 aaaagaggct gaagagaggg ctagagaact tttaagatta gttgatttag aagataaatt
     4261 aaattcttat ccatttgaat tgtcaggagg acagcaacag agagttgcta tagctaggtc
     4321 atgtgcattg atgccaaaag tcctttgttt tgatgaacct acttctgcac ttgatattga
     4381 tactattcaa agagtagtca atataatgaa cagacttaaa gacaaaggta tgacaatatt
     4441 aataattaca catgatgttg tcttctcaaa taatgttgca gataaaatta taagtataaa
     4501 agatggtata gtagaaaatg ttcaaataaa agaaaaaatt gtttagagtt ttataaatat
     4561 aaactagggt agaattttat aatataaata aagggcaagt aaaatcaaat atactatgcc
     4621 cttttgttat ctcaaaattt aat
//
LOCUS       DAENCV010000210         4581 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAENCV010000210 DAENCV010000000
VERSION     DAENCV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.213"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            325..3159
                     /locus_tag="KQD82_003681"
     CDS             325..3159
                     /locus_tag="KQD82_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG4382118.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISSKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDLTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3202..4446
                     /locus_tag="KQD82_003682"
     CDS             3202..4446
                     /locus_tag="KQD82_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG4382119.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1796 a          605 c          687 g         1493 t
ORIGIN      
        1 tataaattat tcttgacctt atcagttttt tcatatatct tcatctatac caaccatata
       61 ttaataaatg atattacata gacttaagta tattaaaaaa cttaaattta gttatttaac
      121 aacataatac tctaatttaa tctataaaaa tccatactta atataatgca actcttattt
      181 taacaccatc tattacacaa aacaacatat taaaaagtaa aatacaaaat atccactata
      241 aaccttattt taataccgaa tatatattat aatatattta atgtagctta aatatagttt
      301 aaacataaag aaaggaaacg gtatatgcta agaaaagata aagttctatt atctctacat
      361 aaattatgtg aaaatatgac tttaaaagac cttcaatcag ataaaacagg attcactaca
      421 atagaaattg ctaaccattc atctcttgat agaagtaata ccagtaaaga attaaataac
      481 ctatacaatg aaaaaaaagt tctgaaaata tctagtaagc caattttatt tctacatgct
      541 agtattatag aaaatttaat tggccgtact ttgaacagtg atgaattttc tataaataat
      601 tgtaatgagc tacttaaatc aagtaaaaat gactcagaag atattttttc aacattactt
      661 ggacatgatt ctagtcttaa actacctata gaaaaagcta aatctgctat actctatcct
      721 ccaaaaggtc ttaatacgtt actcatagga cctactggtg ttggaaaaag tacatttgcc
      781 gaaacaatgt acaaatatgc tgtagaaagt aaaatctttt cttctgaaag caaattcgta
      841 gtatttaact gtgctgaata tgcagaaaat gctaacttat tattaagtaa tttatttggt
      901 tatgtcaaag gttcttttac tggtgctaat aaggataaat tcggcatagt tgcacaggct
      961 gatggaggca tattattctt agatgaaata cacagattac caccagaagg tcaagagatg
     1021 ctttttcttt tgatggacaa aggcatatac agacgattag gagaatctga cattgtgcac
     1081 aaagctaatg tctttattgt gtgtgccaca acagaagata tacattcttc tctgcttggg
     1141 acttttttaa gaagaatccc tgttacaatc aacctacctg ctttacagga taggtcttta
     1201 aaagaaagat tatctcttat taaatacttc tttattatag aatcaaaata caccaatgca
     1261 actataaaag ttcacaaaga tgtaattaaa tcattactcc tttatgattg ttttggtaat
     1321 ataggtcagc ttaaatctga catacaactt atgagtgcaa gagccttctt aaattacaaa
     1381 actggactta aggataatat agaaatagat ttatcccttg ttcctgatta catctatatt
     1441 ggtcttttaa aattgaatga aaatcgtagt aatctaaata aattatcaga acttgattat
     1501 atgctttttt atgaatttaa tggtaataac ttagaagttg aatatacaaa agaactatct
     1561 aaatacaata tagatacatc aaacaatgga tttaaatact ctacagagat tccttatctg
     1621 gaaaatgata taaggagtat gtttataaat tacatggaaa agtactctac aaaattactt
     1681 tcgatatctt cagtaactca ttctgattat acaagtaatg agatttttaa agtaatcaat
     1741 ccaaagattt actatgctgt tgaacattct ttaaaagagg ctgaaaaaat atcgagaaac
     1801 aaatacacaa aacaaactta tattgctctt tcaatgcata taagtgcact tatagaaaat
     1861 ataaatcaag acacatataa tcctaagtac aatttatata atgataaaat tttcttagat
     1921 aaagatttaa atatagctaa aatagtttta agtataattg aaaatgactt acaaacatct
     1981 ttacctaaag atgaagtaaa atttatatcc atgtttctaa atagcatgta tcttgaacca
     2041 aacaatcaat ctgaaagtat aggagtcata atacttgctc atggaaattc aacagcaagc
     2101 agtatgtgtg atgtagcaaa ttcattgctt ggtacaaacc attgtaatgc aattgacatg
     2161 cctcttgatg taaaagtaag taccatactt gataaaacta tagagttggt aaaagaatgt
     2221 gacgaaggta agggtgtact tcttttaaca gatatgggct ctcttatagc ttttggagaa
     2281 cttatttcag aaaaaacaaa aatcaaaact cgctcaattg aaatggtatc tactccaata
     2341 gttttagaag cagtaagaaa aagtacacta ccagaaatga ctttggatga attagctaat
     2401 tccttaagct ctttaaatcc atatattgga cgagcagcaa tcgatttaac agaaataaac
     2461 tatcctgaaa agtcaagaga gtatgtaata ataactactt gtataacagg atatggttca
     2521 gctatgaaag tagcagactt tataaattct tcacttaaaa atattagtaa atacaatatt
     2581 aatttagttc ctcataatat agaaagtttt aaaatgtata agaataaatt tagtaatact
     2641 aaaattctgg cagttgttgg aagtgtagat tttaatgtag ccaacacccc ttttatacac
     2701 atagaggaac tactaaatgg atatggccta gatatgctaa acaaaataat agaaggtaca
     2761 ttcaatgcca atagtattac aaatataaaa gaaacaaatc tatcatacaa cagtttaata
     2821 ataaacttta tgacccaaaa cttagagctt ttggactctg ttaaattgtt caactatgtc
     2881 aattcttcct tatttacaat aatagaatta atagatgaaa acttaaagag caaatttaag
     2941 ctaggatata tactacattg ctcttttatg atagaaagat gttttaaaaa tacaactttt
     3001 ccatataata acattactga attaattagt aaaaatcatg aactatatag tttaatacgt
     3061 gaagccttta agccaacaga agcttatttt aatattttga taccagacga agaaattgct
     3121 tatataatag acatgattaa tgagtatcta aataaataaa atttatagca cttattttgt
     3181 gcatattaat taatatatga aatggaggac gagattttga aaggaaaaat aaaacagtta
     3241 acaatacttg cattaatttt tatatttata actcctgtat ttgcatttgc agatactcca
     3301 ccagtaccaa attcttcaag agcagcacta cttatagacc aggaaactaa aagaatatta
     3361 tttgaaaaaa acattgatga aaaaatgcca ttagcgagtt taagtaaaat gatgaccttt
     3421 ttattggcaa tagaagcagt tgataaaaat caagttaaag agactgatat ggtgaaaata
     3481 gacaagtcta cagcttcagt aggaggttct acttgtaaac ttaaagatgg tgatgaaata
     3541 tctctaggag agcttatgca gggtctaatg ctagtatctg gaaatgatgc tgctatagcc
     3601 atagctaaac atattggtaa aactgaaaag aattttgtaa atatgatgaa caagaaagca
     3661 gaagaaatag gaatgattga tacgtattat tttaatccaa atgggcttcc tatatatact
     3721 gacccagaac ataaagaacc tcctattgaa aatatgtcaa ctgctcacga tatagttaca
     3781 cttggaaagt atatgtacga ccattatgaa aatcaagtta caagaataac cactatgcaa
     3841 gtatataatg atactaaaaa ggattttaca cattataaca caaatcctct gcttgtttca
     3901 gtacctggtg ttgatggaat aaagacagga tatacagata atgctggata ttgccttgca
     3961 ttttctatga tggtacctaa agatgctaaa aacgaaagaa atcatagatt gataggtgtt
     4021 gtacttggtg atggtaacaa gaaaaataga atttcttctt ctgctacttt attgaaatat
     4081 ggtaaagata atttccattc taaaaagata gcacataagg gtgatattat agagactcct
     4141 tgtgttgatg gtattgatga ttttaagata acagttaagg ttgataaaga tttgtatggt
     4201 gttgtttcag ataacgagaa tatcaatcct aaggtagtgt ttaaaaatat gaattatcct
     4261 atccataagg gagatattgt tggtgttgca aagtattata atgactctgg taaatttgtt
     4321 ggaagtgttg atgtaaaaag tgaaagtaat ataggatgta ttcctttgaa ggataagatt
     4381 aagattaagg ttgctaagat aaataaaaaa cttgagatta aaaattctgt ttgttttaaa
     4441 gcatagtaaa gcatattatt aaaagggtat gttaaattta taaagactcc actttgtgga
     4501 gtttttttgt tttttacaaa aaattaaaat agtggcaaca attgaggatg tgagggagag
     4561 ttgccactat tttgtatagg g
//
LOCUS       DAENCV010000211         4568 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAENCV010000211 DAENCV010000000
VERSION     DAENCV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.214"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            76..2487
                     /locus_tag="KQD82_003683"
     CDS             76..2487
                     /locus_tag="KQD82_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG4382120.1"
                     /translation="MRKYKLKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATEPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2784..4361
                     /locus_tag="KQD82_003684"
     CDS             2784..4361
                     /locus_tag="KQD82_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG4382121.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         1839 a          543 c          849 g         1337 t
ORIGIN      
        1 ttttatcaaa ataaaatatg accttaattt agaaatctga tataataaaa attaaataca
       61 tataaggggg taaacatgag aaagtacaaa ttaaaaaaat tgtcaaagct actagcattg
      121 ttgacagttt gctttttaat agtgtcaaca atacctgtct cagcagaaaa ccataaaact
      181 ctagatggag tagaaactgc agagtattct gaaagctatc ttcaatacct agaagatgtc
      241 aaaaatggag acacagcaaa atataatgga gtaatacctt ttccacatga aatggaaggt
      301 acaacacttc gaaataaggg tagaagtagt cttccatcag catataaatc aagtgtagca
      361 tacaacccaa tggatcttgg tcttactaca ccagcaaaaa atcaaggaag tcttaataca
      421 tgctggtctt tttcaggtat gtcaacttta gaagcatatc ttaaattaaa aggatatgga
      481 acgtatgact tatcagaaga acatttaaga tggtgggcaa ctggtggaaa atatggatgg
      541 aacttagatg atatgtcagg gagttcaaat gtaacagcta taggatatct gacagcatgg
      601 gcaggtccta agttagaaaa ggacatacca tataatctta aatctgaggc acaaggtgca
      661 actgaacctt caaatatgga tactgcacct actcaattta atgtaacaga tgttgttcgt
      721 cttaataaag ataaggaaac tgtaaagaat gctataatgc aatatggttc agtgacatca
      781 ggttatgcac attattcaac atattttaat aaagatgaaa cagcatataa ctgtacaaat
      841 aagagagctc cattaaatca cgctgtagcg atagtaggat gggatgataa ttattcaaaa
      901 gataattttg catctgatgt aaaaccagaa tcaaatggag catggttagt aaaaagcagt
      961 tggggagagt ttaactctat gaaaggattc ttctggattt cttatgaaga taaaactcta
     1021 ctaacagata cagataacta tgcaatgaaa tcagtatcaa aaccagatag tgataaaaaa
     1081 atgtaccaac ttgaatatgc tggtcttagc aagataatgt caaataaagt aacagcagca
     1141 aatgtatttg attttagcag agactctgaa aaacttgatt ctgttatgtt tgaaacagac
     1201 tctgtaggag caaaatatga agtatattat gcaccagtgg taaatggagt tcctcaaaac
     1261 aattcaatga caaaacttgc aagtggaaca gtatcatatt ctggatacat aaatgtacct
     1321 actaattctt acagcttacc aaaaggtaaa ggagcaatag tagtagttat agacaacaca
     1381 gcaaatccta atagagaaaa atcaacttta gcatatgaaa ctaatataga tgcatattat
     1441 ttatatgagg ctaaagcaaa cttaggtgaa agctacatac ttcaaaacaa taagtttgag
     1501 gatataaata catatagtga attttctcct tgtaactttg ttataaaagc tataacaaaa
     1561 acatcttctg gacaagctac ttcaggagaa tctttaactg gagcagatag atatgaaaca
     1621 gcagttaaag ttagtcaaaa aggatggact tcttcacaaa atgcagtatt agtaaatgga
     1681 gacgcaatag tggatgcttt aacagctaca ccatttacag cagcaatcga ctctccaatt
     1741 cttttgacag gaaaagataa cttagattca aaaactaagg cagagttaca aagattagga
     1801 actaaaaaag tttatctaat aggtggagaa aattctttaa gcaagaatgt acaaactcaa
     1861 cttagtaata tgggtatatc agtagaaaga atttcaggta gtgatagata caagactagt
     1921 atatctctag ctcaaaagtt aaatagtata aaatctgttt cacaagttgc agtggcaaat
     1981 ggtgtaaatg gacttgcaga tgcaataagt gttggtgcag cagctgctga taacaatatg
     2041 ccaataatac ttactaatga aaaaagtgag ttgcaaggtg ctgatgagtt tttaaattca
     2101 tcaaaaataa ctaagtctta tataattggt ggaacagcta ctttatcatc aaatttagaa
     2161 agtaagcttt caaatccaac aagacttgca ggaagtaata gaaatgaaac taatgctaag
     2221 ataatagata aattctatcc tagctcagat ttgaaatatg cttttgttgt taaagatggt
     2281 tcaaagagtc aaggagactt gatagatgga ctagcagtag gagcattagg tgctaaaact
     2341 gattcaccag tagttctagt tggaaataag ttagatgaaa gtcaaaaaaa tgtacttaag
     2401 tctaagaaaa tagaaactcc tattagagtt ggtggaaatg gaaatgaaaa tgcttttaat
     2461 gaactaaata ctcttttagg aaaatagtga atggaaagat agtaaggata tacattttat
     2521 tattaaaaaa tgttcaatag aagtttataa atataaaata acaaaaaggg gtgtctcaaa
     2581 atgaactttt tagttcatga ggcactcttt tttgttgttt tatattttta tcatttaaat
     2641 aatgaaaaat aggcttgtct atatttttag atagttttct tattattgat gaaaaatgta
     2701 ataattttaa actgtaaaat agtgtaaagt tattttataa tataaatata gtaagatttt
     2761 aaacaaaata agggggaata ataatgaaag caccaaaaac tattttaaca atactgacaa
     2821 tagcgcttac tttaagtagc atttctataa taccatcata tgcacttaca gaggaaaaat
     2881 taataggtaa tggtagatat gaaactgctg taaagataag tcaaaaggca tacagttcta
     2941 gttctaatgt agtgttagtt aatgacaatt cattagctga tgcactttca gccacaccat
     3001 ttgcaaaggc taaaggagca ccaatacttt tgacagaaag tgataagcta gatgatagga
     3061 ctgaaaaaga gataaaacgt ctaggagcta aagatattta tttgatagga ggtactgctg
     3121 tacttaataa agatatagag aacaagttaa aaggtaatgg acttaatgta gaaagaatta
     3181 atggtaagga tagatatgaa acttcactga ttttggctaa taagcttaag gatataaaag
     3241 atattaagga agttgctgta gtaaatggtg aaaaaggatt aagtgatgct gttagcgttg
     3301 gagcaccagc agctcaaaat aagatgccta taattttgtc tagtcctaag gacggagtag
     3361 aagcatttga taaatttata agggatgaga aagttataaa agcatatgta attggaggta
     3421 cgacttcagt ttcaagagct gtagaaaaga gtcttcctaa tgcagaaaga gtgagtggga
     3481 aagatagaaa tgagactaat gctaaggtca tagaaaagtt ttacactgat acaaatttaa
     3541 gtaatttata tgtaactaag gatggtagta aaaatgagaa tcaactaata gactcgttag
     3601 ctgttggagt attggctgca aaaaatgagt ctcctgttgt tttggttggt aataaattaa
     3661 acacaaaaca aagggacata cttagtacta aaaaattaaa tactataaca caggtaggtg
     3721 gaaatggaaa tgaagaagct tttgatgaaa ttaaaagtct acaagaaaaa actgtgtttg
     3781 aagcaaaaac tgttgaggaa ttaactgata tgataaatat agcaagtcct aatgatatta
     3841 taaactttaa accaaaagag aatactgtaa atgaagcttt tagaatggta acaaataaac
     3901 caataactgt gaatataaag ggtgattgtt caaagactct tacagtagat atgcctaatg
     3961 gtgaagtgaa taactatgct actttggtaa atgttatagt tagaaatatt ggagaaggtg
     4021 gatttaataa ccatgataca atcactatat tgtcagttcg tgacaaaaat ggtagagtta
     4081 tagaaaatac tagaaattca gatatagata cgttgatgat attagcttct gctaatgata
     4141 caaaattgat taatgatgga tatataggaa aacttataga taattcatct aatagtgata
     4201 taactaataa tggtactata gataaaaaag ttaatcaggt tgaagattta gaggctaaag
     4261 tagattcaat tgaaaaagct atagattcta taagtcaaaa agtcaataaa atacaagata
     4321 tactagataa gcttggattt ctaaagaaat ttcttagcta agaatatata tatttaagtg
     4381 aagtgtgtgt tgtaattgtt ctaaatttat gtagttaaac aggtttatat acaaatagat
     4441 ttatagataa atatgtagtt tagtatatat ttgaataagc tttggggagg taacatgggg
     4501 attggaattg aatgtaacaa aaaaataaat aaaagtattg acaaaactag aataaagaaa
     4561 aataatct
//
LOCUS       DAENCV010000212         4411 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAENCV010000212 DAENCV010000000
VERSION     DAENCV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.215"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            99..446
                     /locus_tag="KQD82_003685"
     CDS             99..446
                     /locus_tag="KQD82_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG4382122.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(595..1644)
                     /locus_tag="KQD82_003686"
     CDS             complement(595..1644)
                     /locus_tag="KQD82_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4382123.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKVGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            2030..2764
                     /locus_tag="KQD82_003687"
     CDS             2030..2764
                     /locus_tag="KQD82_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG4382124.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(2907..3398)
                     /locus_tag="KQD82_003688"
     CDS             complement(2907..3398)
                     /locus_tag="KQD82_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG4382125.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            3747..4130
                     /locus_tag="KQD82_003689"
     CDS             3747..4130
                     /locus_tag="KQD82_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382126.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKEYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT         1627 a          561 c          619 g         1604 t
ORIGIN      
        1 ataaaagaat tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa
       61 taatagtaaa tctaatatag aaaagaggta tttctagtat gcatgaaaat aaaaagaatc
      121 tattagacgg aagcttagct caatataatg atacagcagt tccaaaaata ccagtatttg
      181 ctggtaacga tataacttca gaagtttatt attttaaacc taatcaagtt cttaatgctc
      241 atagacaccc taatggtgaa caaatatttg tgttcctaaa aggagaaggt aaaatgaaac
      301 ttggtgagca tgaatgtgat gtaaaaaacg gagatactgt atttgtacca acaggagagt
      361 ggcatgaaat tactaatgga agcaatgaag aaatggtagc cgttcaaatt acaaaaatta
      421 atgctggagc agaatacaga ggatagatat ataagtacag ttagtacttt aatatctatt
      481 ttattatata aactttaatc tcaaatataa aaagagtctt ttaaaaggct ctttttatat
      541 ttttgttaat tttcattata aaaaaagaaa atatctagct aaaactaata tattttattt
      601 aatatttttt atagcattgt ttgcaaactc agtatttacg atatctttat aaggtgcttc
      661 cttatctagt tctttagctt ctttcataac agtcatcaat ttcttcaaac cttcctcttt
      721 taatacagga ttttgagacc atgcatctat tgatttatat ctatctacaa ctgttgttaa
      781 atcttctaaa gatatatctt caaagaaagg ttgcatagcc ttagctactt ctttgctaga
      841 tgcttgttga atccataatt gacctttata aacagcattt gtaaatcttt gaattaaatc
      901 tttattgttt ttcatgtatg atctcgtagt agagtatgct gtaaaaggta tttctccaga
      961 atcttttcct atagatgtaa ctatatatcc actaccttct ttttccattg ctgttgctgt
     1021 aggttcaaat gctgttgtaa agtcaccctc tccacctgca aaagctccag ccattacacc
     1081 aaattgtaca tcagttctta cattaacatt gcctactttt agattttctc caatagtaag
     1141 acctttttct tttagaacat attcaaaagt cattagtgga actccacctt ttctaccacc
     1201 taatatttcg gttcctttta aatcattgta agagaaatta ggcatttttt tcctagcaac
     1261 tagaaaacta ccatctcttt ttgtcaattg agcaaagttc actgcataat cgtttttgtt
     1321 ttgattatat acatatatag atgcttcagg acccattaaa cctacgtttg cttcacctga
     1381 aatcaaagct gccatagttt tatctgcacc ttgggtattt ataaggtcaa tctttatacc
     1441 ttcatcttca aagaatcctt tcgtaatagc agcatactgt ggagcataaa aaacagagtg
     1501 agtaacttct gctatagtta ctttatccag atttttagac gaagaagatt cacctttttt
     1561 gtcattagag ttaaaatcac atccagttag acatattgta cttacaatga tagagcttgc
     1621 tattgcgact atttttttaa acataataaa tacctcccat aagacatacg aatagtatat
     1681 agtattaaaa atttattgta gttgttacaa aaatgtatct atgcttataa tatttctaca
     1741 aaaacttaat aataattttc agttttatgt ttagtttttt tagtaatgtg taatattgga
     1801 taaattatat tgattattta taaactaagt aataaaatta gaataatatc acttttttgt
     1861 ctattatata taaggaaaaa ttttttaata atataaatca tattcattta tattattaaa
     1921 aaatttattt atagaaactt ttattatttt atataaaagt gataaaaact tatattttgt
     1981 gtttagtaga gtgttgctaa gtgaatatat ttaaaatgga ggaaacttta tgaaacaaaa
     2041 taaatatgat gatgatagat tttttaataa atacagtaaa atggaacgtt ctacaaatgg
     2101 attagcaggt gctggagagt ggcatgtttt aaaaaaaatg ttacctgatt tcaaaggaaa
     2161 aagagtttta gatttaggat gtggatttgg atggcattgt caatatgcag ttgagaatgg
     2221 agcaatttct gctgttggag ttgatatttc agagaaaatg ttaaaagaag ctagaaacaa
     2281 aacgcaactt gataatataa attacatatg tatgcctatt gaagatatta attttccaaa
     2341 agatagtttt gatgtagtta ttagttcctt ggcatttcat tatattcaat catttgaaga
     2401 tatatgtaag aatataagta attgtctttc aaataaaggt aattttgtat tttctgttga
     2461 acatcctata tttactgcat atggaagtca agagtggtac tgtgataaag atggcactaa
     2521 tctacactgg ccagtagata gatattttga agaaggagta cgtaagtcaa attttctagg
     2581 agaagaggtt ataaaatatc ataaaacatt aacaacttat ttaaatacgt taatgaaaac
     2641 aggatttgaa atattagaaa ttattgaacc tcaaccagaa gaaaaactgt tagatacaat
     2701 tcctgatatg aaagatgagc tacgtagacc tatgatgtta cttgtatctg caaaaaaaag
     2761 ataagtaaat ataaataatc attattgtag aaaaaaagtt gtagaaaata attatactaa
     2821 tggagatgat tataccagtg gtattaatta caccagtagt aaggaggcaa aagcattcgt
     2881 tgcttttgcc attttttaag ttgcaattat acttctacgt agtatatttc gtttccattt
     2941 tcttctataa gctttttcat atcttcaaat tttaaaaata cagtagcagt attatcattt
     3001 ggatgtatac ctattttatc catttttaat aaatccttat caaatacaac ttttacaaca
     3061 gcctctttat catttaatat accaaaagga gtcacagctc ctttagtaag tttcaagtat
     3121 ttttgaagtc tttcttctga tgcaaaacta agaggtttac tatcaatttg ttgacgaata
     3181 tcttttaaat ctgctttttt gtctttacct aatacaacta aaaaatgttc ttttccttta
     3241 gcatcacgaa gaaataggtt ttttacaaca aattccatat gtaatgtttt taattcttcc
     3301 atttcttcaa ttgtataaac tggtggatga tttacagcct cataaggtat gcttaactca
     3361 tctaattttt tatatacttc ctgttgttga ttgttcatga tttcctccca ttagaaattt
     3421 attttatata taaataatat tatcacagaa aactttaaaa gaaaacgttt tatataatat
     3481 agacaataga ttatattttt ataaagaagc aagaatactc aataaaactt aaagaaatta
     3541 aacaatttct aaaaatacct tgaaaataga accttatttg ccatttataa attatataaa
     3601 aagggattat ataaagtcat atatgaaaaa actatattat actatctttt ctttttggaa
     3661 taaactatac tttaaatata gatatttaat ttaaataaat tttacagtat taatagtaaa
     3721 ttatcttaga aaggaatgtg ctttatatgt ctacagacaa agttgttgga atcatagatg
     3781 aagaaacggc agaacttgca ggaattgaat atactggtaa aatttatgct tcttctggtg
     3841 ttataaaaca tataaagaaa aaacatagat gtcaactgag taaagatatt tttaatgata
     3901 taatagacac tataaaaatg gtattaaagt cacctgaata tataggaagt catcctaaaa
     3961 aacctggaaa aagtgtggag tttataaaaa aaattgatga ttatatttta gtagctacag
     4021 aattagatac aaaaaaagaa tatttatatg tatcttcaat gtatgctata aaagaagcaa
     4081 aattagaaag tagagtaaac agtggaagag taaaacaata taatggttaa cataatatta
     4141 ttaattttcc ctaaaatatt gaaaaatatt attatataag ttataatatt aatatacacg
     4201 tagatattta ttagttagat ttgaggttgg aaaaggcacc caatacgcta ctatagagta
     4261 gttaatagag atgcaggata cgccgccctg ccaaatctaa ttaaattaaa aagagaggat
     4321 taattcctct cttattgttt ttttgagata ctaatttaat ctatatagaa agattgaagg
     4381 tcatttatac taaaagtttt cagttatata c
//
LOCUS       DAENCV010000213         4374 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAENCV010000213 DAENCV010000000
VERSION     DAENCV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.216"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            350..1948
                     /locus_tag="KQD82_003690"
     CDS             350..1948
                     /locus_tag="KQD82_003690"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG4382127.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2183..4030)
                     /locus_tag="KQD82_003691"
     CDS             complement(2183..4030)
                     /locus_tag="KQD82_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG4382128.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGNTGV
                     TGNTGVTGSIGPTGATGPTGVTGEIGPTGVTGPTGVTGEIGPTGATGATGVTGSIGPT
                     GATGPTGATGVTGEIGPTGEIGPTGTTGPTGVTGEIGPTGATGPTGNTGVTGEIGPTG
                     ATGPTGNTGVTGEIGPTGATGPTGNTGVTGEIGLTGATGPTGNTGATGSIGPTGVTGP
                     TGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVD
                     ANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVL
                     SGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1362 a          729 c          635 g         1648 t
ORIGIN      
        1 taatttttat attaagaccg ttaatttttt aatgataata tatatacaaa attttttaca
       61 gtataaaata tttataaatt tttagtttat tgacgtatta attaattttt tcccaggtag
      121 atttaatcaa tttattttga ataaaagtaa atttaattcg atatttgatt gggtaaaagt
      181 atatataata ataattttta tattaaattt gtagtattta tgtaagaaat aaattttata
      241 taatattttt tcactaaatt tttttctttt tacaacaaat actcactacc ttttgatata
      301 atatttttat caattattat tatattcaag ttttgaaagg agtattaaaa tgagtttatt
      361 agttttcgga cataaaaatc cagatacaga ttcaatatgt tctgcaattt cattagcaca
      421 tttaaagaac aaactaggaa taaaagcaac agcttatgct ttaggagata tcagaaaaga
      481 agctaaatat gccttggatt actttaaggt agatgcacct gaaattttag ataatgttag
      541 aatacaagtt agagatttaa attatgataa agtagtacct ttaacaccaa cttcatctat
      601 attagaggcc tataacctta tggatgaaaa aaatgttaaa acactacctg taacttatga
      661 tgatggaact tttgctggta tcattactat gaaagaaata gctaaaaatc tacttcatca
      721 acactttaca actatacaca catcactatc aaacatttgt aaaaacctta atggtactat
      781 tcttgtggac tgtggtgaag atataaaagg tagagtagtc actttatcat ttggaatgga
      841 gactctaata gagactttaa aagaaggaga catagctatt gttggagata gatatgatag
      901 tattgaatat gctattgata caaaagttaa acttttaata cttactaatc atacagagat
      961 ttcaaaagat ttgctagctc ttgctaagat aaatggagtg tctgttgttt ctgttgaaag
     1021 tgacaattat aaaacttcta atgtaataaa tcaatgtagt ttcttagact ctatagaagc
     1081 tacagaaaat ttaattacat tcaaagaaga tgattatatg gatgaaataa aagagattat
     1141 gctagaaaca aacttcagat cttatccaat attagatgat gataataagt ttttaggttt
     1201 agtttcaaaa ggtcatctat taaacccatc taaaaagaat gttgttttag ttgaccataa
     1261 cgaatatgct caaagtgctg atggaattga acaagcaaac atagttgaaa taattgacca
     1321 tcataaactt ggtggtattt ctactgatgt tcctatgtca tttagagtta tgcctgttgg
     1381 atgcaatagt actataattt atcaaatgta caaagaaaat aatgttgaga taccttatga
     1441 aatagccggg cttcttttat ctgctatatt atcagataca ttattattta agtctccaac
     1501 aacaactgat atagataaga aagcttgtga agaattaagt aaaatagcaa aagtagacat
     1561 ggaaaaatac gctatggata tgtttaaatg cggtacttct ttagatgaat atactataga
     1621 agaaatagta aatatggact ttaaagaatt taatatgagt ggacatagag ttggtattgg
     1681 acaagtattt accttagaca tagactctat atttgctaaa aaagatgagt ttttatctta
     1741 tataaattcg actgattatg ataaattaat tctttcagtt acagatataa tcaaagaagg
     1801 ttcttattta atctataaag cagaagatag cttaattgct aacgcatttg gtgttgaagg
     1861 tgttcaaggc acatttgcac caggtgttgt atcaagaaaa aaacaattag tccctaattt
     1921 aactactgca atcaaaaatt ttaaataata taaaattttt aaataacgaa aactgtctca
     1981 aatctatatt tgagacagtt ttttattata tatatattga taataaagtt aaataaaaat
     2041 ccataaattt aaaattttta tgaatatatt ttattattat tgttatttta ttcattctaa
     2101 aataggtaaa actcaatata taaaaatact tttgtcattt aaataaagcc taaatagttt
     2161 ttaagattat tttagacacg ttttaagtca taacagtatc agctattcta aatatcgtca
     2221 aagtagccgc agttcctgct gattgcccat ttcgtatccc tactgttgta gatgaaaaat
     2281 tatttattgt aaaagtagtc ccaccaaata aaaaaccagc aaaatatcca gaaagtactc
     2341 ctccgcctcc tggctcagta gtaagccctg ccaaatcttt tcctcctaaa attgggaata
     2401 ggatagcgaa agaatattga tttatacttg atgaacctgg ttgtaatgct actgaaactg
     2461 atatataata cacaccattt tcttgtactg taaacgtacc atttgcatct actgttatac
     2521 ttgaactatt tcctattatt gtttgccaac ctgttatagc atctccatta tttagtgttt
     2581 gtggagatgg tgcatttacg aggaagttag caactggctg agaggaattg cctgttgctc
     2641 ctgttggacc tgttgggcct gttactcctg tcgctcctgt tggtcctgtt actcccgttg
     2701 gccctatact tcctgtcgct cctgtatttc ctgttgggcc tgttgctccc gttagtccta
     2761 tttctcctgt cactcctgta tttcctgtcg gacccgttgc tcccgttggt cctatttctc
     2821 ctgttactcc tgtatttcct gttgggcctg ttgctcccgt tggtcctatt tctcctgtta
     2881 ctcctgtatt tcctgttggg cctgttgctc ccgttggtcc tatttctcct gtcactcctg
     2941 ttgggcctgt tgttcccgtt ggtcctattt ctcctgttgg ccctatttct cctgtcactc
     3001 ctgtcgctcc tgttggacct gttgctcctg ttggtcctat acttcctgtc actcctgtcg
     3061 ctcctgttgc tcccgttggt cctatttctc ctgtcactcc tgttgggcct gttactcccg
     3121 ttggtcctat ttctcctgtc actcctgttg ggcctgttgc tcccgttggt cctatacttc
     3181 ctgtcactcc cgtatttcct gtcactcccg tatttcctgt cactcccgta tttcctgtcg
     3241 gacctgttac tcctgttggt cctatacttc ctgtcgctcc tgtacttccg gttgggcctg
     3301 ttactcccgt tggccctata cttcctgttg ctcctgtatt tccggttggg cctgttggtc
     3361 ctatgcttcc tgtcgctcct gtacttccgg ttgggcctgt tactcccgtt ggtcctgtac
     3421 ttcctgtcgc tcctcttggt cccattggac ctgtaattct aattggaaga cactcacatt
     3481 taggataaca attatctata attctatctt caaatgttac aagtatatac ggtttgttac
     3541 aacctctact agatgtaaat tcaagaatct gataagggta atttaaagat aaagctaacc
     3601 cataattagg atatttattc attgcccatg cttttatagt tcctgtaata tttaaacaaa
     3661 tatagttgga tgtatcactt atcccaactg gtaaatatcc cctaatattt tccatagaca
     3721 ctctatcatt ccatgttata gttcgtaaat tataatattc acttattctt ttaatttcaa
     3781 tatctacttt ttctttaaaa atatgattct tatttgaatc aatatgaata aataactctg
     3841 cttttaatat acttttgtct tttaatatat ctggtattct cactttaaaa aaagtaatca
     3901 acttttcttt tacaaaacta tttgaacatt tacttcctat taagcaataa tctaaattac
     3961 taaagttttt atctggatat tttttagata taaaagtatc atcatttaaa taaagtataa
     4021 tatttctcat ttataatccc tccattttgg gtctatatca tactatgttt aaaatacatg
     4081 cttagttaca atataattaa aatttatagt attaaatttt tatataataa tgctgatttc
     4141 tacaaaaaaa gaacatgtca ttagaaattt ctaatagcat gttctttttc attattatat
     4201 attacacttt aaattattcc ttgactggct tagctggtgt aaactaaacc agtctttcta
     4261 aaatattaag cacttttttc ttattatgtt ttttaggatt tattgtctat attattgtaa
     4321 accaaagtta taaaattgta aatagaatat attaccataa caatatcttt ttat
//
LOCUS       DAENCV010000214         4307 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAENCV010000214 DAENCV010000000
VERSION     DAENCV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.217"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            80..961
                     /locus_tag="KQD82_003692"
     CDS             80..961
                     /locus_tag="KQD82_003692"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG4382129.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            1386..2387
                     /locus_tag="KQD82_003693"
     CDS             1386..2387
                     /locus_tag="KQD82_003693"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG4382130.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            2504..3391
                     /locus_tag="KQD82_003694"
     CDS             2504..3391
                     /locus_tag="KQD82_003694"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG4382131.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            3458..4207
                     /locus_tag="KQD82_003695"
     CDS             3458..4207
                     /locus_tag="KQD82_003695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG4382132.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
BASE COUNT         1587 a          530 c          784 g         1406 t
ORIGIN      
        1 attaaaatta attaatttgt actataatta ttattgtact taaaaaatta aatttagata
       61 aaaaaggaga tttgattata tgttatttaa aggttctgct gttgctttag ttaccccatt
      121 tacagaggat aataatgtca actttgaaaa attaggtgaa ttaatcgaat atcacattga
      181 gaatggcaca gatgcactag ttgtttgtgg aacaacaggt gaggcgacta ctatgagtga
      241 atctgaaatt tttgctgtaa taaaatatac agttgaaaaa gttaataaga gaatacctgt
      301 tatagcaggt actggttcaa acaatactat gttgtctgtt catatgagtc aagaagctga
      361 aaaattaggg gtggatggtc ttttaataat aactccttac tataataaga ctaatgagaa
      421 aggtcttaaa cttcactttg aaacaatagc aaatagcgtt aagttgccaa ttattttata
      481 caacgtacca ggaagaacta aagtgaatat aaagccatct gttgtggctg aacttgctaa
      541 aatagataat atagttgctg tgaaggaagc aagtggagac ttagctcaag ttgcagaaat
      601 agctaaatta gtacctaaag actttgctat atactccggt aatgatgata ctatactacc
      661 actattatca ttaggtggaa gtggtgttat ctctgtactt gctaacatat gtcctaaaga
      721 aacacatgat ttagtaacta aattctttga aggtgatatt gaaggctcaa aaaaattaca
      781 acttgatatg gatgctttaa ttgctgcctt atttatagaa gtaaatccag ttcctgttaa
      841 aactgccatg aacattttag gttttaatgt tggtgattta agacttcctc tagctgaaat
      901 ggaagaaact aatcttaatg tgttaaaaca agaattgaca aattttggat ttaaatttta
      961 gcaatattta tataaataag ttgatttttt cttaaatatg tattattatt agaataatca
     1021 aattttaata tcctattaac ctagggtaga ggtagctgta actaagagta tccaatagta
     1081 gctggcaggt gttaatgatt ggaaaaagga tttacagccg aagaaaatat ttcttgcaat
     1141 aatattttct gggattgcac ataacatgtg taatactgtc acttattttt gtaggtgatg
     1201 agctacaggg tttactttga gtgataaatt agacgtacaa agtatacctt tgtacgtttt
     1261 ttgtatataa ttaaagttgt taatataata ttaaagtttt tggatatata aaaatattaa
     1321 catatcttac ttgtataact aaaataaaat aatgttttaa taatttaaaa ttggggtgaa
     1381 tttatatgaa aaaagtaaat gtagcaatag ttggtgctac tggaatggta ggtagaacat
     1441 ttttaaaagt attagaagaa agaaattttc caatagaaaa tctattttta ttctcttctt
     1501 ctaaatcagc tggttctaaa gtaatgtttt gtggtaaaga atatatagta gaagaattaa
     1561 aggaaacttc atttactgat agaggcatac aaatagcatt attctctgct ggtggaagta
     1621 ttagtgaaaa gtatgctcca atagctgctt caaatggaat cttagtagta gataactcta
     1681 gtcaatggag aatgaatcca gatgtgcctt tagtagttcc tgaggttaat ccagaagcca
     1741 taaaaaatca taagggtata atagctaatc ctaactgctc tactatacaa gctatggttc
     1801 ctcttaaagc tcttgatgat aaatacaaaa ttaagagagt tgtttactct acttaccaag
     1861 ctgtatctgg ttctggtgtt aaaggtgttg aagatttaga gaaaggaatg aacggtaatc
     1921 ctactggatt ctaccctcat caaatttctt acaactgtct tcctcatata gattcattca
     1981 ctgaaaatgg atacactaaa gaagaaatga aaatggtaaa tgaaactatg aaaatacttg
     2041 ataactacga tttaaaaata actgcaacta ctgtaagagt tccagtaaaa aatggtcata
     2101 gtgaatctat aaatgttgag tttaacaatc catttgagtt agatgattta gtaaaaacac
     2161 ttgaagaagc tccaggtgtt gtagtagttg ataatcctgc taaaaacgaa tatccaactg
     2221 ctgtagaatt tgatgataga gatgaagtat ttgttggaag aataagaaga gattttagtg
     2281 ttgataatgg tgtaaatctt tgggttgttg ctgataatat aagaaaaggt gctgcaacta
     2341 acgcaattca aatagctgaa atggctttat catacgattt agtataatat acaaacttgt
     2401 ataaatatat aattttgtaa aatattaata gtaactcaaa aacaagcatt agaagtaact
     2461 gtaatataaa acacaaaaat aaaaaagagg ggattgattt tatatgatat ttaaaggttc
     2521 tgctgttgct ctagttacac catttacaaa ggataacaaa gttgattttg acaaattagg
     2581 tgaattagtt gaataccaaa tagctaatgg tactgatgct atagtatctt gtggtactac
     2641 tggtgaagct aatactatga ctgatgaaga acaacttgct actattaagt atgttgttga
     2701 aaaagttaat aaaagagttc ctgttatagc tggaagtggt tctaatgata ctatgcactc
     2761 agttaactta agtcaagaag ctgaaaaatt aggagtagat gctctattaa taataacacc
     2821 ttattacaat aaagctaata aagctggact taagcgtcat tttgaaacaa tagctaacag
     2881 tgttaaactt ccaattatac tttacaatgt ccctggaaga acttgtgtaa atataagtcc
     2941 tagccttata gttgaacttg ctaaaattga taacatagtt gctgtaaaag aagctagtgg
     3001 agacctagga caggttgcag aaatagctag tttagttcca gatgactttg ctatctactc
     3061 tggtaatgat gatactatac ttcctttatt atctttagga ggtcaaggag ttatatcagt
     3121 acttgctaat gtttgccctc aagaaactca cgatttggta gctaaattct ttgaaggcga
     3181 tattgaaggt tctagaaaat tacaacttgg tatggatgct ctaatcgcag ctttatttat
     3241 agaagtaaat cctataccag ttaagactgc aatgaattta ttgggattca atgttggaga
     3301 tttaagatta cctctagcag aaatggaccc agcaaattta gaagtattaa agaaagaatt
     3361 agttaacttt gggctaaaag ttaatgcata gtaaagatta atgcatggta aaaagtaagt
     3421 ttggtcaaaa gttagccctt aatcatagga ggaaattatg ttaaaagtta tagcaactgg
     3481 ttatgatgga aaaatgggta agattttagc cgatactata agagaagata atgaattaga
     3541 attggtttgt gttgcagcta gaggtctgga tagctatgaa ggcgacctta aaatttatga
     3601 agatatgagt actatagttg aggaagctga tgtagttata gatttttctc atcactctaa
     3661 tttagataat atcttatctt acgttttaaa gactaaaaca cctttagtaa ttgctactac
     3721 tggatataat gatgacgaaa tgaacaaaat acgtgaagcg gctaaagtta ttcctgtatt
     3781 ccaatcatat aatatgtctc ttggtgtaaa tgtattagta aaattggtta aagatgcagc
     3841 taaattacta gaaggatttg atatagaaat aatagaaaaa catcacaaca gaaaagtaga
     3901 tgccccaagt ggcacagctg ttatgattgc taacgctata aaagaggttt tgccaaacgt
     3961 tgaaaataat tatggtagat atggtcgtag tgcaaaaaga caaccaaatg aagttggtat
     4021 tcatgctata agaggtggaa atattgttgg cgaacatgat gctctatttg ctggagacaa
     4081 tgaggttctt acaataagcc atcaagctca atctcgtggt atctttgcaa gtggttctat
     4141 agctgctgcc aaatacttag ttaagcaaaa accaggattt tataatatgg acagcatgtt
     4201 gggataatag tataaatata ataatacaca atataaatta aacatataca atatatatat
     4261 aataatatgc aatacaaatc aaacatatat aataaattta tttaata
//
LOCUS       DAENCV010000215         4296 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.218, whole genome shotgun sequence.
ACCESSION   DAENCV010000215 DAENCV010000000
VERSION     DAENCV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.218"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            224..1303
                     /locus_tag="KQD82_003696"
     CDS             224..1303
                     /locus_tag="KQD82_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG4382133.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEDRMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1627..1995
                     /locus_tag="KQD82_003697"
     CDS             1627..1995
                     /locus_tag="KQD82_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG4382134.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2112..2909
                     /locus_tag="KQD82_003698"
     CDS             2112..2909
                     /locus_tag="KQD82_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG4382135.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGDTSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3308..4198
                     /locus_tag="KQD82_003699"
     CDS             3308..4198
                     /locus_tag="KQD82_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG4382136.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT         1641 a          479 c          808 g         1368 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atagtttaac agtaggaaat atcgtatgag
      121 taagactatc gtattttaca taaagattta gcaatatgac acactgttgt cgtgttctgt
      181 agggtatgat taataaatca gatatgaaag gagagtgagt aatatgccag aattactaaa
      241 aaatatgtat aatcgtgagt cactttatga agttgcagta gctatccaat cagtttataa
      301 ctcatttaag gtagacgagt tcataaaatc tacaatggat gagacatgga ataacttaga
      361 attaaaagcc agatgtcgaa agataagtat gagtttagga atgtatttac cagaagacta
      421 taaggaagct ctttccattc ttgaaaaatc agttacaggt ttttattttg cttttttctt
      481 tccagatttt gttgaagttt atgggcagga cgacataaat tgggatttat ctataagtgc
      541 attagaaaga aatactgagt attggtcttc agaatttgct gtaagagcat ttatcatcaa
      601 ggatgaagat cgtatgatgg cacagatgcg taaatggtct aagcataaga gcgaacatgt
      661 acgaagactt gcaagtgaag gttgtcgtcc ccagttgcct tggggacaag caatatccaa
      721 atttaaaaaa gaccctacac ctgtactgcc tatattggag caacttaaaa cagatacatc
      781 aacgtatgta caaaagagtg ttgctaataa tctaaatgat atctcaaaaa cacatccaga
      841 tttagtcata agcattgcaa aggattggta cggtaaaaat aaatccacta actggattgt
      901 aaagcatggt tgcagaactc tgctaaaaaa aggcaatcgt gatgtattag ccctttttgg
      961 atatgatgat actacttcta taaatcttca ggactttact ttagaaacaa cgtctatttc
     1021 tattggagag gatttaactt tttcatttaa tattttagca aaaaaagcga caaaaacaag
     1081 gctcgagtac ggaattgatt atattaaatc taatggaaaa aggaatagga agatatttaa
     1141 aatatcggag gtttcattaa aagaaaatga gaaaaagtca tatatgaaaa aacattcatt
     1201 tgcagatgta agtgtaagga gacattatcc aggcatccac tcgattgcta ttattattaa
     1261 tggtatagaa aaagataaac ttgattttga attaggtata taattttact aacagaataa
     1321 tctatatcag atactttgct aaacttatat aatatatttt actaaattta tattaggtag
     1381 tttactactt gtttactgaa tttaaataca aagaaataca ggtgttaatc cctgcccatt
     1441 aaaggaatat gagcataaca caaaagtaga acttatcaat tctgttacaa gtacagtagt
     1501 aaatcctcat aatcacaatt agcaaatgaa gtgtaaaaaa taagttagtg ttgattttag
     1561 ttgctagtga tattgaatag ttatacaata gccatataaa ttagaatttg tagaggagaa
     1621 atgtaaatgg atgtatatag tttttggaat gacactttgg cacaagatgc tgttaagata
     1681 agaaaatatt ttcatgaaga tgcttatatt aattggcatg atacaaatga gcattttact
     1741 ttagaagaat ttattattgc aaattgtgag tatcctgata aatggaatgg taaagtagaa
     1801 aaggtgctag aatttggaaa tttagtaatt acagtgacac atgtttatgc aataaataaa
     1861 ccactatcat ttcatgtcgt ttcatttatc agccttaaag atgataagat tatttctatt
     1921 gatgaatatt ggggagagga tgatgatgtg ccacagtgga gattagataa acatattgga
     1981 aagccaataa aatagtgttt gttctgtttt ataactgaaa ttttgataac atttgatact
     2041 ttgatttgtc attggtcaac atagtcgtta gtgtaataat ttaaaaaagt agaatttgga
     2101 gagtgagcac tatggaagaa aaatacatct tatctgaccg aggtaaagta tattattgga
     2161 taaaagaaag taatagtgtg tttgcaaaga acttaatttt tttgccagga ctaacagcaa
     2221 atcatcatct ttttgatttc caaatacagt acttttcaaa aaatcataat gtattggtat
     2281 gggacgctcc tgctcatgga aaatcaagac cttacttaga tttctcatat tctaacttgg
     2341 tagaagaatt gaaagtaatt ttagataaac aaaatatgaa aaaggttgta cttatagggc
     2401 aatcggcagg gggatttgtg gcacagtcct ttattgtaaa atatccaaat atggtagaag
     2461 gattaatgat gattggaact tgtccatatg gaactgacta ttatagtaag tcagatattt
     2521 tctggttaaa acagactgaa tggatgttaa agttattccc agataaattg ttgagaaaca
     2581 taatatctaa gatgtgtgga gatacgtctg aaggcagaag gagtatgatg aaaatgttag
     2641 aaggctacga aaaaaaagaa ctgtgtcatc tcatgtatct gggatttgct gggttcatac
     2701 cagaaattca agacttaaat atacattgtc cagtttgttt ggtggttgga gataaagaca
     2761 agacgggtaa agtgagaaaa tataatgagc agtggcacaa taagactggg tatccattgc
     2821 acattataaa aaatgcttca cataatgtaa atgtcgataa acctaatgaa ctaaatcaga
     2881 ttatggatat atttattaag caattataat atataaaaat tacataccca ttaggtaaat
     2941 tatgtagtat atgtgtttac agatggttat agaaataaat atgtaataaa ctacttaaca
     3001 cttaaaaagt ggatttgtcc agagcataat acaaagcatg atatatataa atttttaagc
     3061 gaaatacatc aaaagaagga ttagaattag tggagacaga acagctatta tagattataa
     3121 ataaatagtt ttaaaggatg ggtactacga atatagagga aggtaaatca tacgttattc
     3181 aaggattata aatgattagt ttaaaagtac tatcggatag gagtctccat atttaaacct
     3241 tagtaaaggt agttaaagta gtagtttaaa aactaaaaaa ttatataaag gaattaggta
     3301 taagaaaatg attgagagta taccagcaaa acaaatttta caaaaagtga aatttgacaa
     3361 tacaagatgg tttggaattg actataatat gaatctctat cgtggctgtt ctcatggatg
     3421 tatatattgt gatagccgta gcacaatata caacatagag aattttgaca aagttagata
     3481 taaagaaaat gtgatagaaa ttttaagtaa agaattgcgt tcaaaaagaa aaaaaggggt
     3541 agtaggcatt ggagctatgt ctgacacata taatcctttt gaaaagcaac tttgtattac
     3601 aaagcaagca ctagatttga tttcggaaaa tcattttgga gtatctattg atactaaaag
     3661 tagtttggtg gttagagata taccgatttt gcagaaaatt aaaaaaaata attctgctat
     3721 tgtaaagttg actataacca ctgcaaatga tgaattatca aagaaaatag aaccttatgt
     3781 aaatccaagt tctgtacgat ttgacgcagt gaaagaactt aatgattctg gaattttttg
     3841 tggcatatta ttgactccta tgcttccatt tttgactgat aataaagatg aaatacgagc
     3901 aattgtagaa aaagcacaca aggctaatgc aaaatttatt tattgcatgt atggcgtaac
     3961 aatgcgtagt ggtcagagag agttttttta tgagcattta agagatatat caccaaaatt
     4021 ggtatttaaa tatcagaaaa catatggttt aaactatgta tgtactgtac aaaataaaga
     4081 ttgttgtgag aagttgttaa gagaagaatg tgcacgttat ggtattttga cggatatgaa
     4141 agagattata aaagcgtaca agaggtcgga gtcatatatt caaataaaat ttttttaaga
     4201 taggctttgg atgagatata atagtggtga ttgatgtgtt gatattatag ttgattgtta
     4261 gagaggatat tatagatgaa tattagtatt gatatt
//
LOCUS       DAENCV010000216         4259 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAENCV010000216 DAENCV010000000
VERSION     DAENCV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.219"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            67..603
                     /locus_tag="KQD82_003700"
     CDS             67..603
                     /locus_tag="KQD82_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG4382137.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            635..1024
                     /locus_tag="KQD82_003701"
     CDS             635..1024
                     /locus_tag="KQD82_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG4382138.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            1061..1447
                     /locus_tag="KQD82_003702"
     CDS             1061..1447
                     /locus_tag="KQD82_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG4382139.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            1574..3016
                     /locus_tag="KQD82_003703"
     CDS             1574..3016
                     /locus_tag="KQD82_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG4382140.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            3313..4128
                     /locus_tag="KQD82_003704"
     CDS             3313..4128
                     /locus_tag="KQD82_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG4382141.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         1639 a          448 c          817 g         1355 t
ORIGIN      
        1 aaatgagata ctatataaat atagaaatag taatcattac tattattaaa aagggaggaa
       61 ttaattatga acttaaaagg aactaaaaca gaaaagaatt taatggcagg atttgcagga
      121 gagtcagaag caagaaataa atatacatac tacgcttcaa aagcaagaaa agaaggttat
      181 aatcaaatag ctgctatatt tgaagaaact gctaacaatg aaaaggaaca tgctaaatta
      241 tggtttaaac ttttacatga tggtatgcct tcaacagagg aaaatttaaa ggatgcagca
      301 gcaggtgaaa actatgaatg gactgatatg tatgcaaaat ttgctaaaga agctagagaa
      361 gaaggatttg acaagatagc ttatttattt gaagctgttg gaaaaataga gaaagagcat
      421 gaagaaagat acttaaagct tttagaaaac ttaaatgaag gaaaaatatt taaaagagat
      481 gaagaagttg tttggcaatg tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca
      541 gaaaaatgcc cagtatgtga ccatccaaaa gcatatttca atataaaagc tgaaaattat
      601 taaaaattat cattattaaa aaggaatgtt aactatgaaa ttttctaaac aacgagaact
      661 gattttaaat gaaatattaa ataatccggt tcatcctact gcggattacc tatatgaaaa
      721 cttaaaaaaa gataatccaa atttaagctt aggaactgtg tatagaaatt tagctcagct
      781 aacggaacat ggctttataa gaaaggttag tattccaggg tatccagata gatttgatgg
      841 tcgaatagat aatcactatc atatcatatg tgaagtatgt ggagaggtat acgatttaga
      901 atccgaagtt cttaataatt tacaagaatt aatatctgag gagacagaca taaaaataac
      961 atcttataac ataagtttta aagggatttg taataattgt aaaaggtgta gccaagtagg
     1021 ttagttttca aattctaaaa ataattaatg aggtgagttt atgtgtagtg aacaaaaatt
     1081 ttttatatgt gagacttgtg gaaaccttgt aggaatgata caaagtggtg gtgtacctat
     1141 tttctgttgt ggaaaaccta tgaaggaact tgttcctaat acaacagatg cagcagttga
     1201 aaaacatgta cctgtaattg aagtggatgg aaataatgtt actgttaaag taagtagcac
     1261 aactcatcca atgactaaag aacatcatat agcatgggtg tatcttatga ctgaacaagg
     1321 tggtcaacgc aaatgtctag cagtagatgg agaacctgta gttaaatttg ctttgaatga
     1381 tgatgataaa gtgatttctg cctatgctta ctgtaattta catggattat ggaaggctga
     1441 actgtaatat ttaaataaat tgagtcaaaa aggaggctat aaatgtattt tcatacaaat
     1501 agcctcctat aaatttatat aaattcaagt tttaattttg tttaatgtca atataatata
     1561 aggagaaaat aatatgtcaa tttataaatg tagtgtttgt ggatatattt atgatgaatc
     1621 taagaatgat aaaacatggg atgaattaag tgaagattgg gaatgccctg tatgtacaaa
     1681 aggtcgtagt tattttggaa agataagtac tgtttactat gaagaagatg aaaagatagc
     1741 agaagacata gttgaagatg aatctaaatt aaatactgaa aaagagggag atttaaatta
     1801 cttaagtaca tatttaagaa gagatgatga agttgagaaa catatggata taattcacga
     1861 aatggctgtt acaggaaaat ctataataga acctatgaga actaaactac cagtaatttc
     1921 ttgggatgat attttaatta tgggtgctca attaaatcca ttacctttaa atgagcatga
     1981 tgaagtaaat acaacaacaa taataggaaa aaaagcaaag aaacctatga ttatagaaaa
     2041 tccagtttat atttcccaca tgtcatttgg agctttgtct aaagaactaa aaatagcttt
     2101 agcaaagggt gcagcccaaa ataagacagc tatgtgtagt ggtgaaggag gtatattacc
     2161 agaagagaag gaagcttcat acaagtatat ttttgagtat gtaccaaata aatatagtgt
     2221 aactgaagaa aatcttaaaa attctgatgc tatagagata aaaattggtc aaggaacaaa
     2281 acctggtatg ggaggtcatc ttcctggcga aaaagtaact gaagaaatag caaaagtaag
     2341 aaataagcct gtaggtcaag atgtaataag tccatcttgt tttgaggaaa tacaatccaa
     2401 agaagattta aagaaacttg tagatgaact tagagaagtt tcagaagggc gtccaatagg
     2461 tgttaaaatt tcagcaggtc atattgaaaa agatatggaa tttatagctt atgctaaacc
     2521 tgattttgta actattgatg gtcgtggtgg agctactgga gcaagtccaa agttgcttaa
     2581 agatgcaact tcaattccaa caatatttgc tctttatcgt gctagaaaat acatagatac
     2641 acatggatta gatatagatt tggttataac aggtgggctt cgtatatcta ctgattttgc
     2701 taaggcaatt gctatgggtg ctgatgcagt tgctattgca agttctgctc ttatggcagc
     2761 ggcgtgtcaa caatatcgta tatgtggttc tggtaagtgt cctgtaggag tagctactca
     2821 ggatgaggaa ttgagaaaaa gattacatat agaaaacagt gctaatagag tggcaaactt
     2881 tttaaatgta agtttagaag aacttaagac ttttgctaga atttctggtc ataaagatat
     2941 acatgattta agtgttgatg atttatatac tgttaactct gaaatatcaa attatacaaa
     3001 catacagcat gtatagattg aagtttgaaa ctattagatt ttagatagct ataaaagtaa
     3061 atgataaata tattttataa aagagagtgt cttaaaatga actttttagt tcataagtca
     3121 ctcttttttt gatgaagcca aatatattta tcattttagc aatgaaaaag aatcttatct
     3181 ctttattttg agatagactc cttctattta tgaaaattaa agatattaaa ttgtaaaata
     3241 ttctgtgatg tcgtactatt aaactaaaga ttctgtactt taaagtaaat aaaaatttaa
     3301 ggagtgatta ttatggaaag attgaagata caagttttag ttgacaataa tacttatatt
     3361 gatagatact ttgttggaga accagcagta agctattata ttgagattga tggaaatagg
     3421 attttgtttg actcagggta ttcagatgta ttcatatcaa acgctgaaaa attaaatata
     3481 gatttaggaa atttaactca tgttgttttt tctcatgggc ataatgacca tacaagaggt
     3541 atccagtttc tagaaaatag gtatgattta tctacagttg aacttatttc acatcctaac
     3601 tgttttatac ctagaaaaca tggagagaaa agcataggag ctccattttc tgctgaggaa
     3661 attaaaaata tttttatgta taatcctaag gataagccat ttaatctaag taagaattgt
     3721 gtgtttttag gtgaaatacc ttctataaat gattttgaaa aaagagctaa aattggtaaa
     3781 tgtaaaatag gagatttgtg ggaagatgat tatgttttag atgattctgc tatagtttgt
     3841 aaaactgata aaggtatttt tattgttaca ggatgttctc atagtggaat ttgtaatata
     3901 gtagagtatg ctaaaaaggt atgtggagat gatagagtta ttggtatact aggtggtttt
     3961 catctttttg aattgaataa taggttagat agtacaatac aatatttgga taaagaagaa
     4021 atgagaatgt tgtacccttg tcactgtgtt tcttttaagg taaaagcgaa gatgaatgaa
     4081 atattgaata taaatgaagt tggagtaggg ctgaccataa gtatatagta tttgaaagta
     4141 tataagattt aagttgaagg ttaaaaagat attttctata gaaaatatct tttttgttac
     4201 gtttatttta agagaaatca atatactttt atatgaaata ttgtaaaaaa atttatttt
//
LOCUS       DAENCV010000217         4250 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAENCV010000217 DAENCV010000000
VERSION     DAENCV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.220"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            215..1354
                     /locus_tag="KQD82_003705"
     CDS             215..1354
                     /locus_tag="KQD82_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG4382142.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEGIEILSKEKNIYKVNDKYLKMLDID"
     gene            2352..>4250
                     /locus_tag="KQD82_003706"
     CDS             2352..>4250
                     /locus_tag="KQD82_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
                     /protein_id="HBG4382143.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSGGSSGGSSSSKVGQIKFSATDKQIKMASGDKTVDPSDNTYILKLTNGTVKDGDVKA
                     NVKVAGLPSGLDYTAVGNRSANTITITVSGTASQSIQNDLNNVSVLVKAGAVSGTGAT
                     DSIANTTFE"
BASE COUNT         1820 a          437 c          689 g         1304 t
ORIGIN      
        1 agatattata atatgtaact aatgtattat aaataatagt tgtaaacttc ctgtgccata
       61 tattttaaat gcttattctt aaatttttgc attatttatt aaaatttatt atgctttaac
      121 gctaaattga gtttgataat tataaaacaa ataatcaaaa atagtgtaaa aattaatact
      181 ttttgttaat attaatagat aaagataatc taaggtggtg attaaactga caatagaaga
      241 atacaaaaac aaatacttta ctggaaataa aatcgtactt tctataaata gttttgataa
      301 taaagaacta tactatcatg aaactgaaaa tatattaaat ttaataaatg aaatagataa
      361 attaacatca agtaagatat ttaaaataat agaagataaa aatgatttgg cactagagtc
      421 ttattattct agtgtaatag aaggtgcttt ttcaaataga aagattgcaa aatctataat
      481 tagaggaaag atgaaaccta gtaataaaag tgaatatatg atttacaata atcatagagc
      541 cttagaatat ggattagaca acttagataa attatatagt cacaaattta tatatgattt
      601 gcatcatata ttgggcgaaa actgcttgga ttcagaagaa tatgagtata gaactgaaaa
      661 agtatatgta tgtgattcaa aaggtgagat tatacatata gggcttgaac ctttgaagat
      721 atatgacttt atgagtaaat taatagattt tatggaaaat agcaaagtaa gtaatttaat
      781 taaaagtgct ataattcatt tttactttgt atatgttcat ccttttagtg atggtaatgg
      841 aagaacatca agagccttat catatctata tttgattgat aaaggatatg atactttcaa
      901 ggagttttca atatcatata tgatatctaa aaacagaact aagtattata aggctatact
      961 tgatgtagaa aacaaaggaa ataatttaac tgtatttata gagtttatgc tcaagtctat
     1021 aatacaatct attaatgaga tgcgtaatat gcatgataga aaatctttag aaagtatatt
     1081 aaaagaagaa ttgtttgaaa atgacataac tctatcagca acagaagaac atatattaaa
     1141 atatatttgc aataaagata actattctat gacattagaa aattatataa aaaagaataa
     1201 aagtagatac ttaaaggctg gaataaagga aattgaatta gttgaccagt tgatggaagt
     1261 gtttaataat cttgaaggga tagagatttt gtctaaagaa aaaaatatat ataaagttaa
     1321 tgataagtac ttaaaaatgc ttgatataga ctaatttaga taatctggag agggaatata
     1381 taaataataa gaaaaaggca tttttgattt ctggggctat aaacgtaatt tcattaatta
     1441 ttgttttttc attgtttcat tataatatta tagaaaatag tacacaaaat atgataataa
     1501 ctgtaatctt aatttttatt tttgaaatga taaaaataaa aatcatagag aaatactata
     1561 aactataaac aagaaatatc ttttctaaga gcatattgta caatatgctc ttaatttagt
     1621 tatatagatt taatataaat aatgtatttt ttgtagatga tttatgtaag atttatgacc
     1681 aatttgtgta tgatttgtgt tctttaatca tgacaatatt atattgtaaa aaataaattt
     1741 aataaaaaat gacttggaat ttatattcta ggtctttttt atataaaaat ttaataagtt
     1801 tcaaatgttg gtaactttga cagtagaaaa cgtctattga gataacgagg attctaattg
     1861 agggggagat atattagcac ttaaagtagt atatgcaaca gatataatct ttataaaagt
     1921 aattcgaatt aaaatgtaaa tataaagaat aattaaaaaa atatcgcaat tatttgtttt
     1981 tcatatggat aaaattggtt atttaatata taattacaag tataaataat aaaatttatt
     2041 tgtaaaaaga gtagcgcttt taattctaaa ggctactttt tttatacaaa accctatttt
     2101 ttataacaat tcattaacat aaaaatcaaa cttaccttaa aatataaatt tacaaatttt
     2161 tcaatcaata agaaaatttc tttattcctt acataatgac tgcaatgtaa caaaataatt
     2221 acaaattaat tatgaaatca ttatagttag aaacctcttt aaaattagat attaagtatc
     2281 ctttagaatt agaacgggaa caaagttaaa aaattaagta atatcaaatg gacaaggggg
     2341 aagaaaataa aatgaaaata ccaaagaaga ttgctgcaat gcttacagtt acaatgatag
     2401 ctggaagttc aacagcaggc atagctagtg cacaaactgt ggcaacaaat ttaacagggc
     2461 aagaaagata tgaaactgct gttaaaataa gtcaagatgg atggaaaaat gctgatgagg
     2521 tagtaatcgt aaacgactca tctatagcag atgctctatc tgcaacacca tttgcaaaag
     2581 caaaaaatgc tccaatactt ttaacaagta aagataaatt aaatgataag acgaaagcag
     2641 aaatacaaag attaaaagct aaaaaagtat acttaatagg tggaacttct gtattaagta
     2701 caaatataga aaaagaaata aaagatttaa aaatatcttt tgagagaatc tctggagctg
     2761 agagatatca aacatcctta gaattagcaa agaaattaga tgctataagt gatgttaaga
     2821 aaatagctgt agttaatggt gaaaaaggac ttgcagatgc tgtaagtgta ggagcaccag
     2881 ctgcacaaaa caatatgcca gtaatacttg ctgattctaa aaatggaaca gcagttgcag
     2941 acaaatttat aaaagatgct ggaataactc aatcttatgt agtaggtgga gagagttcaa
     3001 tttctgaagc tgttaaaaat aaactaccaa atagtacaag actaggtgga acagatagaa
     3061 atgatacaaa tgcaaaagta ataaaagaat tttataaaaa gacagattta aaaaatgctt
     3121 atgtaactaa agatggtatg aataaacaag accaacttat agatgcacta gcagtaggtg
     3181 tattaggtgc taaaaatcaa tcaccagtag ttttagttgg aaagaactta tcagcttcac
     3241 aaaaatctct agttaactct aagagttttg ataagataac taaagttggt gggaatggta
     3301 atgagacagc atttaatgaa atgaagtcat tacaagaagt aaaaacagtt gaagctaaaa
     3361 caatttctga attaaaatca gctatagata aagctactgc taatgatgtt ataaacttta
     3421 agccaactag tgaggttaaa gaagcattta caatacaaac tgataaagca gtaacagtaa
     3481 acttaaatgg tacatataca aagactgtga ctatcaacat gcctaatggt gatgtaaata
     3541 actatgcaaa agtggatgat gtagttatag atgatgtaaa agatggaact tttgttaact
     3601 acggaaaaat aactaactta aaggttaatg ataaaaatgg agccaagata gaaaataatt
     3661 ctaaaggtga gattggtagt ttaacagtag catctggagc aagtcaagta aaagtgacta
     3721 atggtggtaa gataactaca gttacaaata attctaaagg gactactata gataataaag
     3781 gtacaataag tagtgttaag ggagataatt ctccaacaat aagtggaaat agtccaagtt
     3841 ctaattcatc aggaggaagt tcatcaagtg gaggttcttc aggaggaagt agtggaggaa
     3901 gttcttcatc aaaggtaggt caaataaaat ttagtgcaac agataaacaa ataaaaatgg
     3961 catcaggaga taagacagta gatccatcag ataacacata tatactaaaa ttaacaaatg
     4021 ggacagtaaa agatggagat gtaaaagcta atgttaaagt agcaggatta ccatcaggac
     4081 tagattatac agcagtagga aatagatcag caaatacaat aacaattaca gtatcaggaa
     4141 cagcaagcca atcaatacaa aatgatttaa ataatgtaag tgtgctagtt aaagcaggag
     4201 cagtatctgg aacaggagct acagattcaa ttgcaaatac aacatttgaa
//
LOCUS       DAENCV010000218         4120 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAENCV010000218 DAENCV010000000
VERSION     DAENCV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.221"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            168..2267
                     /gene="nrdE"
                     /locus_tag="KQD82_003707"
     CDS             168..2267
                     /gene="nrdE"
                     /locus_tag="KQD82_003707"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG4382144.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEAFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2280..3251
                     /gene="nrdF"
                     /locus_tag="KQD82_003708"
     CDS             2280..3251
                     /gene="nrdF"
                     /locus_tag="KQD82_003708"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG4382145.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3327..3953
                     /locus_tag="KQD82_003709"
     CDS             3327..3953
                     /locus_tag="KQD82_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG4382146.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1645 a          452 c          768 g         1255 t
ORIGIN      
        1 atagataaag cactgactat atgtagtgct tttgtttttg aaaaatactt attgcatttc
       61 tataattata gattaatttt gggatttcaa ctgataagta ccaaaagaaa taataagcaa
      121 attaagtgaa attatggcac aagattatgt taaaaggacg gtaaataatg aattggatag
      181 agcttaacaa tcaagtgata attaaaaatg aacaaggcaa gtaccaattg gaaaaggata
      241 aggaagcctt aagtagttac atagaagcat ttatacagcc aaagttgaga aaatttaata
      301 acctagaaga aagattgaag tatttaatag atgaaggtta ttattcaaaa gaagtaataa
      361 atcagtatag catagatgtt attaaaaata tatatgatat gattgataaa cataagtttg
      421 aatttcaatc atatatgagt gcaaataagt tttatcaaaa ttatgcatta aagagcaatg
      481 atggaaagga aatattagaa acatataatg ataaagtgtt aattgtagct ttgacactag
      541 gaaatggcga taaagattta gctttaaact tggcagataa aatagtaaaa caagaatttc
      601 aaccagctac acctactttt ttaaatgctg gaagaaaaag ggctggagaa atggtatcat
      661 gttttttact atctgtagaa gatagtacag aaggtatatc gtatgctata tcttcatcaa
      721 atcacttatc aaagattggt ggaggagttg cactaaattt atctaaactt agagcatctg
      781 gcgaatctat caaagatata gaaggtgcag ctggaggtgt tgttggagta gctaaaatgt
      841 tagaacaatc atttagttac tttaatcaaa tgggagctag acaaggttct ggtgcggtgt
      901 accttactgt attccatcct gattttgaat tgttgatgga tactaaaaag ataaatgcag
      961 atgaaaaaat aaggcttgct acattatcat taggagctat aataccagat aaatttatgg
     1021 agttggcaga aaaaaatgaa gtagcatatg cattttatcc tcatactgtg tataagaaat
     1081 atggagtttc tcttgatgag atagaaatgg atgagtggta tgataaattg gttaatgata
     1141 gtgatataag aaaaaaagaa ataaatccta ggcagatgtt gacaaaaata gcacagatgc
     1201 aacaagaaag tggatatcca tatgtagtgt acatagatac agctaataga gaacatacat
     1261 taaaagatgt tggaattatt aaaatgtcta acttatgttg tgagatattt caatatcaaa
     1321 caccttcaga aatagaggga tatggaggaa aaaatgaatg gggacaagat ataagttgta
     1381 acctaggttc tttaaacata gccaatgtaa tggataataa aactatagaa agtacagttg
     1441 aaacagcaat aagagcactg tcatttgtag cagatagcac agatataaaa ccagtgccaa
     1501 cggtaagcaa tagtaacagc aagtctcatt ctataggatt aggagctatg aatttacatg
     1561 gatacttagt aagagaaaat attttatata cttcagatga tgctatagat ttttctaatg
     1621 tattttttgc aatggttaga tactattcaa ttaaagcttc tatgaaaata gctatagaaa
     1681 gaaatcaaac ttttgaagga tttgataaat cagaatatgt taagggaaga aatagcaagg
     1741 ttttatcaaa atactatgaa caatcttatc tacctaagtc agaaaaggta agagcattat
     1801 ttgaagggat ttatatacca acaaaagaag actggacaaa attattggat gaggtaaaag
     1861 aaaaagggat atataatgca tatttaatgg cagtagcacc aactcaaagc attagttatg
     1921 ttcaaaatgc tacatcaagt ataatgccaa ttactgagcc agtagaagta agaacatatg
     1981 gagactctac tacaatatat ccaatgccat ttttaacaaa tgataatatg ttgtattatc
     2041 aatcagcata caggatggat atgagaaagg tcatagattt agtggcaaca gttcaaaacc
     2101 atgttgacca aggtatatca acaacactgt ttgttacaga tgaaaagaca actagagata
     2161 tagctagaca ttacatatat gcctataaaa aaggtttgaa aagtttatat tacacaagaa
     2221 caaagatgac aagagatact catgagtgtc tagtgtgttc agtataggga gagaaatata
     2281 tgacatttaa tttacataaa atacataatg cagttaactg gaatagagaa gaagatggat
     2341 ttacacaagc tttttgggaa caaaatgtaa aacaattttg gcttcctgaa gaaatatctg
     2401 tttctaaaga cataaaggta tggaatgaac taagtaataa agaaaaggaa ttatataaaa
     2461 aagtattggg tggtttaaca ttactggata caaaacaggg aaataatgga ataccatcta
     2521 tgatgagttt aacagaaaac ctacaaagaa aagctgtctt gtcttttatg gggactatgg
     2581 aagaaataca tgcaaaaagt tattcttcta tatttatgac tctactttct aatttagaaa
     2641 tagatgaatt atttgaatgg atagaaacag aaccaacatt acaaagaaag gctgatttag
     2701 ttttagctca atacgaaaat actacaaatc aagagggatt atacctatca atggttacaa
     2761 gtgtattttt agagagtttc ttgttttatt caggattctt ttatccattg tatctatctg
     2821 gtcaaggaaa gatggtagct agtggagaaa taatatcgct tattcttaga gatgagtctt
     2881 tacatggtaa gtatatagga ttgctagcac aagaaatata cgattcattt gataaaatgg
     2941 ataaggagat gttagaagag aagatgtatt ctatacttta tagccttatg gagaatgaga
     3001 tagagtatac aaatgttata tacagagaaa gtgggcttga aaaagaggtt gtaaactttt
     3061 taaaatacaa tgcgaataga gctttagaaa atctagggtt tgagaagcta tatactgtag
     3121 atgctataaa tccaattgta ttaaatggtc ttagcactga aaccaaaaca catgatttct
     3181 tttcaactaa gggtaatgga taccaaaaag gagtgtatga ggaattagag gatgaagatt
     3241 ttataatata atttataatt atgacacact gttgtcactt ttagcgtgat aatatcaatg
     3301 tataaaatat atatggaggt atttttatga aatatgaatg gagaaaaaga gataaagagt
     3361 tatatttacc taaaaagaat cctgtaatag ttgaaatacc agaacagaaa tttatcatgt
     3421 taaaagggag tggagaccca aattctaaag aatttactga agcaatagga gtactttatt
     3481 ctttggcata tgcaataaaa accatgccta aaaaaggaat tgtaccagaa gggtattttg
     3541 actatacggt ttttccttta gaaggtatat gggataaggg agaaaaaagt aaaggcttag
     3601 atgtacttat taaagaagat ttaatttaca ctataatgat acgtcagcca gattttgtta
     3661 cacaagacct gttggataaa gctactgata tagtaaagaa gaaaaaacct cataatcttc
     3721 ttgaaaaagt tagatttggg tctattaatg aagggctatg tgttcaaatg cttcatatag
     3781 gttcatatga taatgaacca gaaagctttg acattatgaa cgaattttgc aaaaaaaata
     3841 aacttataag aaaatcttac acacatcgtg aaatttatat aagtgatgct agaaaagtat
     3901 cgcctgaatc attgaagacg gttttacgtt ttgaagtaga aagggaagta taaatatttt
     3961 gaataattta taagagttat aaaaagtgaa gtctgttgag acttcatttt ttgatgcaat
     4021 tttaaaatta aatttgatgt ttcataatat tttaaaaact ttctactttt attagtgtga
     4081 ataactgttg ctattaaata ataatactaa ttatataagt
//
LOCUS       DAENCV010000219         4120 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.222, whole genome shotgun sequence.
ACCESSION   DAENCV010000219 DAENCV010000000
VERSION     DAENCV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.222"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            156..1559
                     /locus_tag="KQD82_003710"
     CDS             156..1559
                     /locus_tag="KQD82_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4382147.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1650..1890
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1933..2631
                     /locus_tag="KQD82_003711"
     CDS             1933..2631
                     /locus_tag="KQD82_003711"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG4382148.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2654..3655
                     /gene="trpS"
                     /locus_tag="KQD82_003712"
     CDS             2654..3655
                     /gene="trpS"
                     /locus_tag="KQD82_003712"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG4382149.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1553 a          457 c          733 g         1377 t
ORIGIN      
        1 taagatttga tttcaaagta tttaaggttt attattaaaa ctgtttttga atatgcaaat
       61 attttgaaaa atatcagttt aataataatg aatctatttt attaattttg aaattaaatt
      121 taaaataatt aatagagagg ggaaattaaa ttaggatgga aaagaaaaaa ataggcctat
      181 ggagtttggt atttatgaat gtatcagctt tatatggtat aagatggata gctaaatcta
      241 cagcatcaag ctttggttta ggattaggtg caatacctat gtggttatta ttctcattta
      301 tatactttgt accaggagct ttaatttgtg ctgaattagc ttctacatat caaaaaggtg
      361 atggaggtct ttacgattgg gttaaggaag cctttggaga aaagtatgga tttttagttt
      421 cttggttaaa ctggacagct aaaatatttt ggtattcttc atttttaact ttcttagcag
      481 taaatgtttc ttatacaata ggaaatccag cccttgctga taataagatg tttgtactta
      541 tattatcttt aataatattc tgggcattat ctcttatagc aactaaagga ataagtttta
      601 caaaaatatt tactaataca ggtgcattag gttcaacaat accaagtgct atacttataa
      661 tattttcatt tgtagcagta tttgtgttga aaaaacatga tatagcttct acatacacaa
      721 tagcttcaat gactccaaaa cttaatatgg atgcatttgt tgctatatca gctataatgt
      781 ttggtttagc aggagcagag actgctgcta acttcataac agaaatagat aagcctgaaa
      841 agaacttccc taaggcaata ttaatatcag ctggaatagt agcttcatta tatgtattag
      901 gttcaattgc aataacaatg ataataccac cagaccaaat tactgcttct aaaggtgtat
      961 tagatgcact ttctgctgtt tgtgcgtctt taggaattgg ttcttggttg gttcaattaa
     1021 tagcttttgg tatagctttt tcagtattag gagcaatagt tctttacata gcatcaccaa
     1081 taaaaatgtt atttggtagt gttaaaaaag gaatatttcc agatagttta gtagaggtaa
     1141 atgaacacaa gataccttct aaagcagtta tacttcaagc aataatagtt actataatac
     1201 tattggtgac tacattaatg ccttctgttg atgctatata caatgttctt gtaactatga
     1261 cagcattaac tgcattattc ccatatgtat tgttatatgc ttcttatatt aaactaagaa
     1321 aagagagacc agatgaaatt agaccttata ctatggcaaa atcaacaagt acttgcctag
     1381 gtttagcaaa aatggtttta gttgtaactg tagtgggtat attattatct gcagcaccag
     1441 ttatgcctac atttgctgaa aatgttatat atgagataga gatgattggt ggaggtcttc
     1501 tagttatatt atctggtcta tggttatgga atagatatga aaagaaaaca gctaaataaa
     1561 atgtatttta aaaatttctt ttggatatta taatatatag ttgacaattt atagtgtaag
     1621 gaatattata aaataaagaa attaataaat taaatcctga gataagaaat agtaaataaa
     1681 tatatttttg cacagagaat taaggatgct gagaacttaa taaaaagtat ttattgaatg
     1741 ggtctttgag gagtaaaatg aaatgtaatt taagattaca gagtatttga tatcgtgtat
     1801 gcacgttata gcatatatta agatatatat attcaatttt gagtgtatat aaatttaggt
     1861 ggtaccacgg aaaatatatc cgtcctattg atttaggacg gattttttaa tatagaaaat
     1921 ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa tggatgaaga agttagtatt
     1981 ttagttgatt taatggatat tagagagact ataaaaaaag ctagcttaga attttataag
     2041 ggaattttgg aaggtaagaa tgtagtatta gtaaagtgtg gcataggtaa ggttaactct
     2101 gctctttgtg cccagatact aataagcgaa tttaaggttg atgctattgt taatacaggt
     2161 gttgcaggag ctttaaacga aaaacttgat gttaatgata tagtaatatc aactgatgct
     2221 atacagtatg atgtagacac tacagcattt ggagacccta agggagttat accaagaatg
     2281 aagacttctg tatttaaagc agatgaaaga cttatagatg ctgcttacaa atcttctgtg
     2341 gaagaagtaa agactcataa agtattaaaa ggaagagtag ttacaggaga taaatttata
     2401 aattctaaag aattaaaaga agaattagta aatgattttg gtggatattg tggagaaatg
     2461 gaaggtggag ctatagctca tgtatgctat ttaaataata ctccatttgt aataataaga
     2521 gcaatgtctg ataaggcaga tggaagtgct gatgttactt atgatgtatt tgtacatgat
     2581 gctgccaata actctaaaga catagtttta aatatgttaa aatcaatata aatatataag
     2641 gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt ggagcacagc catcaggcaa
     2701 aatgacttta ggaaattatt taggagctat aaaaaactgg actgaacttc aagacaatta
     2761 tgattgttat tatagtatag ttgatttaca tgcaataaca gtacctcaag accctaaagt
     2821 tttaagagcc aatactatag aattacttgc tcaatatata gcttgtggat tagacccaga
     2881 aaaaaatact atatttatac aatcacatgt caaagagcat gttgaactta tgtgggtatt
     2941 gaatactatg acttatatgg gtgaattaag tagaatgact caattcaaag ataaatcaca
     3001 aaagagtgaa gcaaatctaa atgcaggttt attcacatat ccagttctta tggctgctga
     3061 catattgttg tatcaaactg acctagttcc agttggagat gaccaaaaac aacatttaga
     3121 attagctcgt gatttagcaa atagatttaa taatagattt tcacctactt ttgtagttcc
     3181 agaaggatac tacccaaaag gtggagcaag agtgatgagt ttgcaagagc caactaaaaa
     3241 aatgagtaaa tccgattcaa atgaaaatgc atttatacta ttagcagatg attcagattc
     3301 tataaagaga aagataaaaa gaagtgttac tgattcttta ggtgttgtaa aatacaatga
     3361 tgaacaacca ggtattaaaa atctaattga tatatattct aatttaagta aaaagagtgt
     3421 agaagagatt gtaaacatgt atgaaggaaa gggttatggc atatttaaag aagatgtggc
     3481 tgaagtagta tcagaagctt taagacctat aagagaaaaa tatgttgacc ttcttaataa
     3541 taaagattat ttagaaaaaa tatatagtat gggagcagaa aaagccgaaa aacaagctag
     3601 aaaaactctt agaaaagttt ataaaaaagt tggatttata gagagaagat actaattatt
     3661 agctatcttt acttgataaa taaattttaa agttattaaa aaggtatatg atatgaaaga
     3721 gagagtctca aagtgaactt ttgttcatga ggcactcttt tttgtatgaa ataaaatata
     3781 ttttaatcat ttcaacaatg aaaaagagga ttatctctat tttgagacag catcttttga
     3841 taataaaaaa agatgagctt ttgctcatct caaaggggtt agattattct tactttgggg
     3901 aaagtagaat aaaggtaaaa gttaactgat gtcaactttg tcttttttgc atatcactac
     3961 tgagcataaa aaaatcagaa aaccatagcc ttctgaaaca acttctaaca tacattgtat
     4021 tttataccca atatgtaatt tatgagttaa ttttaactct tataatgaaa attgtcaata
     4081 atatatcaaa aaaatatttt ttaaatttat ataattaaat
//
LOCUS       DAENCV010000220         4117 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAENCV010000220 DAENCV010000000
VERSION     DAENCV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.223"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..302)
                     /locus_tag="KQD82_003713"
     CDS             complement(<1..302)
                     /locus_tag="KQD82_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382150.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALE"
     gene            complement(420..2243)
                     /locus_tag="KQD82_003714"
     CDS             complement(420..2243)
                     /locus_tag="KQD82_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382151.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2256..4031)
                     /locus_tag="KQD82_003715"
     CDS             complement(2256..4031)
                     /locus_tag="KQD82_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382152.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1340 a          732 c          368 g         1677 t
ORIGIN      
        1 tcttcaagag ctattctctt ataagcttct cctacttctg gataaccttc tctatcagct
       61 tgtctactca ttgctaaata cattcctact tctgtacact caccaacaaa gttagctctt
      121 aatccttcga ttatttcttc atctacacct tgagctactc ctattctgtg ctcatcagcc
      181 caattttttt cgcctttcat ttcttcaaat ttatcagctc caactttaca tactggacat
      241 tgtgctggtg cagcatctcc ttcatgtata tatccacata ctgtacaaac aaatttcttc
      301 ataattactt tcctcctaaa ttttatatat caaattaaat tttttattta ttaagttagt
      361 ctatatatac ccgcaccata tacctttaaa caattttttt aaaagttttt tctaaatact
      421 tattttccct gtgtcaataa tatgtttcta taataatgtc cctttaattt tatcaattct
      481 ttatgatttc ctgattccac aattctacca ttttctaaaa ctattatatt atcacaattt
      541 gatatagtac ttaatctatg agctataatt atacttgtct ttccataagt taaattattt
      601 attgcttctt gaattttagc ttctgttgct atatctatgt tacttgtagc ctcatccaaa
      661 ataagtattg gagcattttt taatactgct ctagcaatac ttattaattg tttttgacct
      721 tcacttaaat cttcacccct atccgataat acagtactat atccttcacg cattttaact
      781 ataaatttat gtgcatttgc tattttacta gcatatataa cttcttcatc agtagcctcc
      841 agatttccat accttatatt ttccataact gtatcattga atatataacc ttcttgtgga
      901 actactccaa aacaagttct caaatagttt ctgttaatag agtatatatc cttaccatct
      961 aaaaatatac ttccttcatc tatatgataa aaatttgtaa ttagatttat aatagttgtt
     1021 tttccacttc ccgtctctcc tatgatggca tttgatgtgc cagatttgat ttttaggttt
     1081 atattatcta aaattttctt ttttgaatca taagaaaaac ttacatcttt aagctcaatt
     1141 tctcccttta caaaatgaga gtttaaatta ccatctttat tatcgttaat ctcactttta
     1201 aacatgttat gcacttcttg ctcttcttgt tcatctataa tttcaaaaac tctttcacat
     1261 ccagctatgg ctgaaagtaa tgtattaaat agatttgcta attcatttaa aggtcttgtg
     1321 aattgtctta cataacttaa aaaactagct ataaccccta tggttataat atctttagat
     1381 gcaagaatgc ctcctataat acatatagtt gcaaatccta tattactaat cacattcata
     1441 ataggcatta aaagagaagt gtaaatttgc gatttaaggg aaatatttag aagtctatca
     1501 ttgacttctt taaaatcatt tacagatttt ctttcataat taaaagattt tactacattt
     1561 atgtttgata tcatttcttc aatatgtgca tttagttttc ccaactcaac ttgttgttgc
     1621 ttaaaaaaaa ctctagttct ctttgcaata aaattactca caaacattac tatcggtatt
     1681 aatattacac tgcaaaaggt taaaattggg cttaatacaa acatcataat aaaagtcatc
     1741 aatatgttta agattcctgt aattaactgt actaatgaat ttgatattcc tgtactgata
     1801 ttatcgacat catttgtcac tctactcata atatctccag tagaactttt atcaaaaaag
     1861 gttattggta gatttttaaa ttttaaaaat aaattacttc tcatttctct gactatactt
     1921 tgagataatc tagctgtaca tatcccctta gacagtttaa ctgaaaactc aattatataa
     1981 aatgaaagta aaataattat tccttttaag aaaatggatt ttgaataagt atcgatatct
     2041 attatgtcaa ctacctttcc aatagtatat ggtattacag ttgatataat tgaatcaatt
     2101 attactagca aaaaaataaa tctagtaatc ttcttatctt ttttaaagta cataaaaatt
     2161 cttttaaacg tttttttaaa atctttaggc ttttcttttt tctttaatct actgaccttc
     2221 tgatttccag tacttaaatt caatatactt cctccttaat ttacatcttc acttactaaa
     2281 ttttgcaacg caaaaatttt tttataaatt ggagaactaa ccagtaaatc ttcatgtgta
     2341 gcaaatccaa ccacctcacc ctcatccaag actaaaatct tatcacagtc aattatagaa
     2401 ctaatctttt gtgaaatagt tattacagta catttcttac tttccttttc tcttaaatta
     2461 cttatatact cttttatatt ctttcttata attgtctctg tattaatatc cattgcacta
     2521 aaacaatcat caagaattaa tatttgtggt tttttggcta tagctctagc tatagtaatt
     2581 ctttgtctct gtcctcctga taaattttta cccctttgga ataaaacttc atcataagta
     2641 ttttcgagtt ttgttataaa ttgatttgca tttgctattt cacatgattt tacaacatcc
     2701 tctttgcata tatctctatt tcccataact acattctcca atatactttt tccaaacaca
     2761 ctactatttt gaaatactac acctattctt gcccttaaac tttcttcaga aatactgtat
     2821 atactacgtc ctccaatctt aatatcacct ttatcaatta tatataattt ttgaattaaa
     2881 tttactaaag aagtctttcc tgaaccagtt tgaccaatca caccaatatt ttgaccttct
     2941 tctattttaa aggatacgtt ttttaatatt ttttcatctg tatttttgtt atacgaaaaa
     3001 tctacatttt caaaaattat atcacagttt acaaacttta aatctttttc tccaacatct
     3061 aaactttctt ctctctcaat tacttcttca ttctcatcaa ccaaaacctc acttattctc
     3121 tcataagatg cttttgccct aacaaaaaca ttaaaaacat gtgaaatcat tataagagat
     3181 gttaatattc tagtcatata gtttacataa gcaactataa tcccaatttt aatttctcct
     3241 gaataaatta aatgccttcc aatcagtaat attattatta tggaaacatt aatgattatt
     3301 gtaattgtcg gtctaaaaaa tgacatcata gttgtagctt ttctactatt tttaaaaagt
     3361 tcatcattta actcttcaaa gctttggttc tcataatcgt atctacaaaa tagtttaact
     3421 acttttattc cacttaaaaa ctgtcttaat tttgaattta ctttatctgt actttcttgt
     3481 acatttttaa ataatctata ccctatcttc atattaagca atattataat aaatataatt
     3541 aaaactgcaa ttagaataat tattgacatc tttaaattta attttatagc tataattaaa
     3601 cttcctatac acattattgg tgactttata aaaactctca tcattccatt aacgaattgt
     3661 tgcatttgat tgatatcatt agtaattctt gttattaatg aagctctacc aaatttatct
     3721 atttgtttta atgagtatgt atttatcttt tcaaatacat cctgtcttaa atcataagaa
     3781 aaatgctgag aaactcttgc cgagattaca cttcttatta ttgcaaatat tgcacctaaa
     3841 aaagttatac ctatcattat agctcctgtt gatagtatgt agtctaagtt cttatttgaa
     3901 acccctatat ctattatttt tgccattata gttggttgag ctaaatcaca aaaagcttcc
     3961 aatgttaaaa aaaatactgc tgttagaaat aatactatat atttttttat gtatttttta
     4021 aaatacctca ttcaatcatc ccttcataga attttctcat taccaaaagt atatcataat
     4081 ggctaattta attgattata tcaattataa atatttg
//
LOCUS       DAENCV010000221         4102 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAENCV010000221 DAENCV010000000
VERSION     DAENCV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.224"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..282
                     /locus_tag="KQD82_003716"
     CDS             <1..282
                     /locus_tag="KQD82_003716"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382153.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            540..1589
                     /gene="carA"
                     /locus_tag="KQD82_003717"
     CDS             540..1589
                     /gene="carA"
                     /locus_tag="KQD82_003717"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG4382154.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1782..>4102
                     /gene="carB"
                     /locus_tag="KQD82_003718"
     CDS             1782..>4102
                     /gene="carB"
                     /locus_tag="KQD82_003718"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG4382155.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         1701 a          415 c          809 g         1177 t
ORIGIN      
        1 aatagcttaa gagaaaatgg aatagaggct gatatagtaa atagagtaga agaatctaga
       61 ccaaacatat tagatgctat aagaaataaa caagttgata tagtaataaa tacaccaact
      121 aaaggaaatg actctacaag agatggattt aagataagaa gaacagctat tgaattttct
      181 actgaaataa tgacatcttt ggatacacta aaagctttag tagaagttaa gaagaaacat
      241 ttaaataaag atgagttaaa agtttacaat atagctgaat agaaattaag ttgattgtat
      301 agtttaagaa tttaattatt aaataaaaaa aaagaggtta ttttttctag gagtttttac
      361 ttttgagata acttcttttt tttaagaaat ttgtagtata atagaaaaag ataaaaaata
      421 aatgtaaata agtataaaac tagaatgtaa aagtgaaaaa tagatataga tatttaaaag
      481 cttatattta gttaatgttc tttagtttgt ctaaagagaa ttataggagg tactgtagta
      541 tgaaagcaag gttaatatta gaagatggaa cagtttttat aggaaaggca tttggatatt
      601 tagaagaaag tgtaggagaa gtagtattta atacttcgat gataggatat ggtgaagttt
      661 taacagaccc ttcttactat ggtcaaatag ttactatgac ttatcctctt gtaggaaatt
      721 atggtataaa tttatcatca gcagagtctg aaaaagttca agtaaaggga ttcatagtaa
      781 gggagaaaag tgattctcca agtaatttta gatgtgaaat tgatatagac caatatttaa
      841 agcaaaataa agttatcgga cttgaaggaa tagatacaag agctttgact aagatactta
      901 gaaataatgg aacaatgaaa ggtataatta cattagaaga cagtaaatta gaagatgtta
      961 aacataaatt ggataagttt tcaaatacag aagcagttag aactgttaca agaaaagaag
     1021 tagagcatat aaaaggaaat ggtccaaaag tggctgttat ggattttgga gtaaaaagaa
     1081 atatattaag atcatttata gctcgtggat gtgatataac aatatttcca gctacaacat
     1141 ctccagaaga tgtattgagt ataaaccctg acttaatatt cttgtcaaat ggacctggag
     1201 acccagaaga cttagaagat gttatagaaa atataaaagc tcttattggt aaaaaaccta
     1261 tagttggaat atgcttagga catcaacttt tagctttagc tcttggagga aaaactgcta
     1321 aacttaaatt tgggcataga ggtggaaatc atccagttaa agacttagaa gaaggaaaag
     1381 tatttataac ttctcaaaat catggttatt atgtttcaga agttcctgaa caaatgaaag
     1441 tgacacatat aaatttaaat gataatactg ttgaaggaat gagacatgag aagctagatg
     1501 tatacagtgt tcaataccat cctgaagctt gcccaggacc aaaagataat gactatattt
     1561 ttgataaatt tttagaatta gtaaaataaa caacattaga atagctaact aaaaaacaag
     1621 aataacttaa atcaaaaagt ctggtttaat taacatagat gtattaaata gatgtattga
     1681 tgagtaaatt tgaacttaag ataaaataaa gtaattaaag ttgattgtat agatgatgaa
     1741 gataagaact gtgaaaattg tttagataag gagaaagagt tatgcctaaa ttagatagta
     1801 taaagaaaac tttagtatta gggtcagggc caataataat aggtcaagct gcagagtttg
     1861 actattcagg aacacaggcc tgccaagcat taaaagaaga gggaattgaa gttgtattag
     1921 taaatagtaa cccagctact ataatgactg ataaagaaat tgcagataaa atatatatag
     1981 aaccattaac aatagaattt atagaaaaaa taatagaaaa agaaaaacca gatagtttac
     2041 ttgcaggtat gggtggtcag acaggattaa acttagctgt agaattacat gatgctggta
     2101 tattagataa atataatgta aaagttatag gaacatctat agaatctata aaaaagggcg
     2161 aagatagaga tttatttaga gaagtaatga aagaaataaa tcaaccagta atagttagtg
     2221 atatagttac aaatttagaa gcaggtcttg agtttgccaa taagatagga tatccagtag
     2281 ttgtaagacc agcatatacg ctaggtggaa ctggtggagg tatagctgat actgaggaag
     2341 aattaagaga aatactttca catggactac agctaagtcc tgttggacaa gttcttcttg
     2401 aaaagagtat aaaaggttgg aaagaaatag aatatgaagt aatgagagat ggaaatggaa
     2461 actgtataac tgtatgtaat atggaaaatg tagacccagt tggagttcat acgggagaca
     2521 gtatagttgt tgctccaagt caaactctaa gtgatgaaga gtatcaacta cttagaaagg
     2581 catcaataga cataataaat gcaatagagg ttcaaggtgg atgtaacgtt caaatagctt
     2641 taaacccaca tagtttagaa tatgcgataa tagaaataaa cccaagagtt agtaggtcat
     2701 cagccctagc atcaaaagct acaggatacc caatagcaaa agtagcagct aagatagctt
     2761 taggttatac tttggatgag atagaaaatg cagtaactaa aaaaacttat gcttgttttg
     2821 agccaacatt agattatgtg gtagtaaaaa taccaaagtg gccatttgat aaatttagac
     2881 atgccaatag aaaattagga actaagatga tggcaactgg tgaaatcatg agtataggaa
     2941 gtaactttga ggctgctatt cttaaaggta taagatcact tgaaacagga aaatattcat
     3001 tggttcatgc tccatcagaa ggtagaacat tagaagaatt aaaggctaga gtagtagtac
     3061 ctgatgatga gagactattt gacttagctg aaatgataag aagaggctat aaagtagaaa
     3121 tgatatcaga aataactggt gttgataagt ggtttataaa taaatttaaa tggatagtag
     3181 aacaagaaga aaaattaaaa gttctaaaaa tagaagactt agataaagaa tatttacatg
     3241 aattaaagaa aaagggattt tctgataaag gtatatctga cttaatgaag ataagcccag
     3301 aaaaattata tgaattgaga agtttatata atatacaacc agtttataaa atggttgata
     3361 catgtggagg agaatttgaa gctctatcac cttattacta ttcaacttat gaacaatatg
     3421 atgaggtagt agttagtgat aagagaaaag ttgtagtatt gggttctggt ccaataagaa
     3481 tcggtcaagg aatagaattt gactattgtt cagttcattg tgtaaaatct ttaagaaaaa
     3541 tgggaataga aactataatt gtaaacaata accctgaaac tgtaagtacg gattttgata
     3601 catctgataa gttgtatttt gaaccattaa cagaagaaga agttttaaat ataatagaaa
     3661 aagaaaaacc agaaggtgta atactacagt ttggtggaca aacagctata aaattggcta
     3721 agtttttaca tgaaaaaaat atacctatac tgggaacaga ctttagagat atagatgcag
     3781 cagaagatag agaaaaattt gatgatttac tagagagact agatataaat agaccaaaag
     3841 gaaaaggtgt atggacaact aatgaaggtg ttgaaattgc taaggagtta ggttatccag
     3901 tattagttag accatcttat gtacttggtg gtcaaggtat ggaaataact tataacgaag
     3961 aaaaactaat tcagtatttg gatgatgcat ttgatagaga ccataaaaat ccagtactta
     4021 tagataaata tctaactggt agagaaatag aagtagatgc tatatgtgat aaagaagata
     4081 tattgatacc tggtataatg ga
//
LOCUS       DAENCV010000222         4042 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAENCV010000222 DAENCV010000000
VERSION     DAENCV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.225"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            162..1115
                     /locus_tag="KQD82_003719"
     CDS             162..1115
                     /locus_tag="KQD82_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG4382156.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRANIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1105..2061
                     /locus_tag="KQD82_003720"
     CDS             1105..2061
                     /locus_tag="KQD82_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG4382157.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2058..2813
                     /locus_tag="KQD82_003721"
     CDS             2058..2813
                     /locus_tag="KQD82_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4382158.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2905..3870
                     /locus_tag="KQD82_003722"
     CDS             2905..3870
                     /locus_tag="KQD82_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG4382159.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1640 a          380 c          670 g         1352 t
ORIGIN      
        1 taaattttaa tgttactaga agtttcaatt ctagtaacat tttttatgtt aaaaacagat
       61 atagatatgt attgacaatg ataattatta ttgataaact ataaaagtga aaatgataat
      121 aattttcatt ttaattaata aaagattttt aaggagggag aatgaagaaa aggtatttag
      181 tcataggaat aataattttg tcttattttt ctttatttat aggagctgaa gatataaata
      241 taatgcatat atttgccaaa aatcagcata aacttatgat atttatcatg agtagaatac
      301 caagattaat aagtatattg attgctggtg taggaatgag catagcaggt cttataatgc
      361 aacagataag taagaataaa tttgtatcac ctacaacagg tgctacaata gatgcagcac
      421 aatttgggat agtaatatgt atgcttttag taccaactgc ctctattttt acaaagacaa
      481 taatagcatt tgtattttct cttgtaggga cttttacgtt tatgaaaata atagggaagt
      541 tacaatttaa aaatataatc tttgtaccat tagtaggtat aatgtttgga aatataatag
      601 gttctatgac ggattttatc gcgtataaat acgatttgag tcaaaatgtt agttcttgga
      661 tgcaaggtga cttttctatg atacttaaag gaaattatga gatattatat atcacaatac
      721 cccttataat attagcatat atttatgcaa ataaatttac tgttgttggt atgggtatgg
      781 attttgcgac aaacttaggt cttagttaca aaagaattgt caatataggt ctgataatag
      841 tagcacttgt tacagtatgt gtagtcgtta cagctggtaa tatccctttt ataggtctaa
      901 tagttccaaa tatcgtatcc ttgtatatgg gtgataatat aagagcaaac atatggtata
      961 ctggtttact tggagcgata tttgtactta tatgtgatat ttttggaaga attattattt
     1021 atccatatga aatatctata gggcttacag ttggagttat aggaagtata ctattccttt
     1081 atttaatatt aaggaggaat gtaaatgaag cttagtctca atcaaaaaat ttactttgta
     1141 agtatattac tattcatatc aatagcactt tttataattt atggaataga catgaatcat
     1201 ttagaatatg cattatcaca aagaatacca aaaatatttg ctatggtatt aggtggagga
     1261 tgtatagcat ttactactgt agtgtttcaa acaattacaa acaatcaaat attaacacca
     1321 agtgtactag gtttagattc attgtatgta atgattcaaa ctataatagt attcatattt
     1381 ggttcaagca gtagtctgat tataaatgaa aattataatt ttattattaa tgtagctatg
     1441 atgattggag cttcattgtt attatataag atgttatttg aaaaaaataa aaataatatt
     1501 ttctttttaa tcctagtagg aatgatattt ggaaccttat ttaaaagtgc aacaacattt
     1561 attcaggtaa tgatagaccc gaatgaattt ttagctttac aaactagtat aatggcaagt
     1621 ttaaacaata taaacactaa tgtattgtta atagcattta taataattat tgccataata
     1681 ccttttatat atgatgaaat aaagtattta gatgtattgt cgttaggtaa agaacaagca
     1741 attaatttag gtgtagattt tgataaagca gttaaaaaaa tgataatatt aatagcaatt
     1801 ttagtctcaa tatcaacagc acttattgga cctatgacat ttttaggact tttacttgct
     1861 aatataacaa gagaaatttt taaaacatat aagcatacat acttgatttc gggttctatg
     1921 ttgataggga tgattacatt gataataggt caatttttta tacaacatgt atttaagttt
     1981 gatacaactt taagtgtagt aataaatttt ataggaggaa tttactttat tcaattacta
     2041 ttaaaggggg caaatagatg atagagataa aaaatatatt taaaagatat aagaataaaa
     2101 atgttgtaga tgatgtctct tttagtatag aaaagggaaa gataacatct tttataggac
     2161 caaatggagc tggaaaaagt acagtactat caatagttac aagacttata ggaggtgatg
     2221 gtggagaagt aataatagaa ggaaaaagct tgactaatta tagcaataaa gaattagcaa
     2281 aaaaaatagc tattcttaaa caatctaaca atataacttt aaagcttaca ataagagaat
     2341 tagttggatt tggaagattt ccatatagtg aaggtaatct aactaaagag gatgaaaatt
     2401 atattgatga agcaatagaa tatatgaagt tgactgatat acaacataag tatttagatg
     2461 aattgagtgg aggtcaaaga caaagagctt atatagcaat ggtaatagct caagatacag
     2521 agtacatatt acttgatgaa ccactgaaca atttggatat gaaccattcc gtacagatga
     2581 tgaaggtact tagaagtctt tgtgatgagt tagataagac tatagtatta gtaatgcatg
     2641 atataaattt tgcatcatgt tattcagata atatagttgc attaaaaaat ggaaaagttg
     2701 aaaaagtagg tcgtacagat gagatagtaa atgaaaaagt tttagaagat atttatgaga
     2761 tgaattttaa cattaagaat ataaatggaa atcgaatctg tatttatttt taaaatattg
     2821 tatatgaaaa taaacttgct atataatttt ttataaagtt tttaaattaa aataaagatt
     2881 aataggagag aggaaaatat tgatatgaat aaaaaagcag caatagtagc agcagtagct
     2941 attataggat tagtgacagt gtttgcatta ggtggaagta aaaagaatga atcaaaaaca
     3001 tcagaaaatt caaataatac cataaagata actcataact tgggagaaac agatgtaaaa
     3061 ttaaatccta aaaaagtagt agtatttgat tattcggctt tagatacaat ggatgcatta
     3121 ggtgtagctg agaatttagt aggacttcca aaagcgagtt taccagcatc tttagaaaaa
     3181 tataaggatg acaagtatgc agatttagga ggattaaaag aaccagattt agaaggtata
     3241 aagtcggcaa atccagattt aattataata aatggaagac aagaagattt ttatgagcaa
     3301 ttgtcaaaaa tagcaccaac aataagtaca agtaaagatg ataagaagta cttagaatct
     3361 gttaaaaata atatagacaa aatagcaaaa atatttggag tagaagaaaa agcaaatcaa
     3421 gaatttagta aaattgaaaa gaaaatagaa actttgaata aaaaagtaac agataaaaat
     3481 ttaaatgctt taactataat ggtaaatgaa ggtaatttaa gtgtatttgg tgaagaatca
     3541 agatttagta tattatacaa tagttttgga tttgaaaata aagataaaaa tataaaagaa
     3601 tcaagtcatg gacaaaatat tacttttgaa tatatagcta aacaaaatcc agaagttatg
     3661 ttcgttatag atagaggaat agctactggc agtgatgtaa aagaaagctc aactgcaaaa
     3721 tctgttttga acaatgatat aataaaatct atggatgcat ataagaatga taatataata
     3781 tatttagatt caccaacatg gtatgtaaat gatggtggtc taacatcttt aaataaaatg
     3841 atagatgatg cttcaaaagc ggtaaattaa taatccgata taaccaaaat gtatttttta
     3901 taaaatgatt atcaaataaa ctaggagttt ttaagaactc ctagtttatt ttttgcataa
     3961 aaaagatata gtttaaacaa taatattatc tataataaga taaatctatt caaaatctaa
     4021 taaaatatga agttatacta ga
//
LOCUS       DAENCV010000223         4038 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.226, whole genome shotgun sequence.
ACCESSION   DAENCV010000223 DAENCV010000000
VERSION     DAENCV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.226"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            425..1681
                     /locus_tag="KQD82_003723"
     CDS             425..1681
                     /locus_tag="KQD82_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG4382160.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSEVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1744..2937
                     /locus_tag="KQD82_003724"
     CDS             1744..2937
                     /locus_tag="KQD82_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG4382161.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            3138..3518
                     /locus_tag="KQD82_003725"
     CDS             3138..3518
                     /locus_tag="KQD82_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4382162.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1401 a          415 c          738 g         1484 t
ORIGIN      
        1 attttaattg ataggttata ttatttataa cattaaaaat acaatttaca taaaatagac
       61 atatgaaaaa taaaaaatta tataataatt atgggaatga ggttctccct tggatgataa
      121 tatcctaaac tgccaatgag ctgatgactt ctatgatatc atagatgcca ccagcttttt
      181 ttgttgttta aatcaataaa atggaggtga agtacttact aaatttttat tttttaaatt
      241 tgaagaaata tttaattaat agaaaaaata gtagattata tgttaattat tttattaaga
      301 atgggttaag agtgtaccta tatcctatca atttaaaaaa caattatgat agtcattaca
      361 ttgactacag ttgatgcaat aaaactttca aaaaatctta tgatgcaaat agaaaggaat
      421 taaaatgata gaaaaattta aaactttaat atggattaaa atgataataa gaattaaaag
      481 cgtttataaa acatatggtg gactattttt tttaggactc ataggtatac cagttggtgc
      541 aattataggt cttatagaca ccatatttgg aacggttctt ctaaaagtta cagatattcg
      601 agaaacatat ccaatgtatc tgataccatt tttagcagta gtaggagttg ttattgctta
      661 ttgctatttt aaatttggag gaaaaagtag taaaggaatg aatctaatat ttgaggtagg
      721 gcatggagaa gaagaaatta ttcctttaag attggttcca tttataatat caggtacatg
      781 gttaacccat ttatttggtg gtagtgcagg acgagaagga gttgctgttc agattggtgc
      841 aactttttca cattgggtag gtaaaaggtt gcctataaaa aatgcttcta gtatttttct
      901 agtaactggt atggcagctg gttttgcagg attatttgag acaccaatag cagcaatact
      961 atttgctatg gaggttttgg ttgctggttc actggagtat caatcgcttt ttcctgcgtt
     1021 tactgcttct tttacagcaa gtgcagtatc aaaagcttta gggttagaga aattttcttt
     1081 tgcactttct tctgaagttg tatttgactt gtcaatattt tggaagctta ttgttttagg
     1141 aattattttt ggaatggttg gtggagcatt tgcatggtgt ctgaaattgt caaaaagaaa
     1201 aatagggaat agattaaaaa atcctatgat aaggattgct attattggag tatgtcttag
     1261 tgtattattc cttttatttt ataaaggaag atattcaggt ttagggacaa atttaattca
     1321 aaatagtttc tatggtggag aaatttattc atttgactgg ctgttaaagt ttatacttac
     1381 aatactgact ttgtcagcag gttttcaagg aggagaggtt acacctttgt tttccattgg
     1441 agctagtctt ggtgtattgt tagcaggatt ttttaatttg ccaattgaat tagttgcagc
     1501 gcttggctat gctagtgtct ttggtagtgc tacaaatact ttttttgcac ctgtatttat
     1561 aggggcagaa gtttttgggt acagctattt accttatttc tttgtagtat gcgctatttc
     1621 atatatattt aatatggata aatctattta ttcactacaa aaaatttcaa caaaacaata
     1681 aataaaacaa cataatgtat gattagaaga gaaagaatat tagagaaagg atagggtagg
     1741 aatatgttga caagtttagc tttgattttt ttattaggaa tggcctcggg aggtattttt
     1801 aagaggataa agttacctaa cttattggga atgttactta caggaattat attaggtcca
     1861 tatgttttaa atttgattga taattcaata ctagatattt cttctgacct tagaaagatt
     1921 gcattgatta ttatattaac aagggctgga ttatctttag atataaacga tttgaagaaa
     1981 gtgggaagac ctgctgtact tatgtgcttt attccagcta cttttgaaat tatcggaatg
     2041 atagttttag caccaaagtt actaggcgtt tctatattgg aagcagcagt catgggtgca
     2101 gtagtaggag cagtttctcc cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa
     2161 ggttatggta cagaaaaaag tattccacag atgcttttag caggaacttc tatagacgat
     2221 attttcgtaa ttgtgatgtt tacagtattc acagggttgg cacaaggaaa tagtatatca
     2281 gctattagtt ttttacaaat tccagtatca attatattag gtgtaatagc tggagcagtg
     2341 attggaatct gtttagcagt attttttaag aaggttcata tgagagattc tgctaaaggg
     2401 gtgttgcttt taagtatctc atttttaatg ataagtttag agactgcttt agagggtatt
     2461 gtgccatttt ctggattatt agcagttatg agtattggga ttttccttca gataaaatat
     2521 cgtgtagttg caagaagatt gtctataaag tattcgaaac tatgggttgg tgctgaaatt
     2581 ctcttgtttg ttttggtagg ggcaacagta gatataagtt atgcatttaa agcaggaatt
     2641 ggagctgtaa ttttaatatt tggagttctt ttattccgta tggtaggagt attcttctgc
     2701 cttattaaga caaatttgac aataaaggaa aggttgttct gtatgattgg ctatattcca
     2761 aaggctacag tacaggcagc aataggaggt gtaccacttg caatggggat ggcatctgga
     2821 caacttattt tgacgttagc agttctagct atacttataa ctgcacctct tggagctttt
     2881 ggaattgatg taacttataa aaaattatta acttctgtaa aaggcgagag taagtaaaaa
     2941 taaagattat tttaactggt aagttatatt ttggaaaagg attatactat tgaatacaat
     3001 ataaaaaaat catttgggag attaagaagg tagaagttaa gaacagattt taaggtatgt
     3061 tatgattatt agaattgatt ttgtgaaatt attattgtaa tatttaatta tatagataaa
     3121 gaagcggagt gataactatg tgtttaaaaa aattatcaaa attagagtta gttattatga
     3181 aatttatttg gaatttagat ataaaaacca attcatatga gattattgat tatatgaaag
     3241 aagaacataa tttaccagaa aaagtagctt taaaaacttt atcaaaacta tcaaagaaaa
     3301 gattcttata tgttcaagaa acaggcaagt gtatgtatta tacagttgca ataaaagaaa
     3361 aggcatattt agagtttata tcaagaaatg tgcaaaatct tttgaagaat aattttataa
     3421 ggaatttatt agtatcattt cacgaagagg agctaacaga ggaaaagata aagtcgttag
     3481 aaaactgggt agtaaattgg gaagaagctt atgtataaat gttattattt ttattacaat
     3541 ttagataatt ttggttttgt aagctctgca actattttta gatggttgca gagtttattt
     3601 ttatttttta ttcccttaag caatacttaa aagtaaacgc atttatattt actaacttca
     3661 attacttcta atacttaaat tactttttaa aaatattttg atatgtcatc tctatagtaa
     3721 tctagaactt gtttctattc tttatataaa gtttttatga atgatatata taatattcgt
     3781 tcatttgatt aaaaataaca aaatattaaa taattctact ctgatagttt gttaaaaaaa
     3841 taataaaaaa tattaataaa caaaaaaaaa ttatcttaag agaggagaat gttctaaaat
     3901 ataaaaaggt ttctagattt cataaaagat actattttag tcttgaaaat atttagtttg
     3961 aaaagatttt aatttaatga ttgattaagt taaaaatgtg tatgtaataa attttatttt
     4021 atttattgac taaattat
//
LOCUS       DAENCV010000224         4033 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAENCV010000224 DAENCV010000000
VERSION     DAENCV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.227"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            129..1019
                     /locus_tag="KQD82_003726"
     CDS             129..1019
                     /locus_tag="KQD82_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG4382163.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1237..2523
                     /locus_tag="KQD82_003727"
     CDS             1237..2523
                     /locus_tag="KQD82_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG4382164.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTKKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2612..3055)
                     /locus_tag="KQD82_003728"
     CDS             complement(2612..3055)
                     /locus_tag="KQD82_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG4382165.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3079..3873)
                     /locus_tag="KQD82_003729"
     CDS             complement(3079..3873)
                     /locus_tag="KQD82_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG4382166.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         1555 a          473 c          636 g         1369 t
ORIGIN      
        1 agttacttga catagtttac acttttaatt atagtataaa ttggaatatt tagataatta
       61 ttatttaatg tataagctaa gtttttaatt aatatggctt tatacattta tatagaaaag
      121 aggtacgtat gagtagcaca atcaaatcaa atcgaataaa aggtataatg tttataatag
      181 catcagcgct tggctttgca atgatgtctg cattcgtaaa gctagctgga gatttaccaa
      241 gttttcaaaa agtgttcttt agaaatctgg tatcagctat aatagctttt tggcttatca
      301 taaaacataa aggaagtcta actggtaaaa aagaaaattt aaaagtactt ttatataggt
      361 caatatttgg aactctagga gttatattta actactatgc aattgacaag ttgatgcttt
      421 cagatgctaa tatgctaaat aaaatgagtc ccttccttgt agtaatattt tgtgcaattg
      481 ctctaaaaga aaaaataaat gtaaagcaga taggagctat tataattgca tttataggag
      541 ctttattcat aataaaacca acatttagtg tagaagtaat tccatatctt gggggaattg
      601 caggagctgt atttgcagca atggcatata cttgtttaag agttcttgga gatagagaag
      661 attactatac aattgtattt ttcttttcag ttttttcatt agttactgta gggccaattg
      721 cttttgcagt atatgaacca atgaccctta tgcaacttat atatctatta ctaggtggag
      781 tatttgcaag tctaggtcaa tttggaataa ctctagcata taaatatgca cctgctaagg
      841 aaatctctat atttgactat agtaatataa tcttttcagc aattttaagt atattcttat
      901 tcaatgtcta tccagatatg ttaagtgttg ttggatattt aatagtattt tcggcagcat
      961 tttatatgtt tttatacaat aagaagttag ataaattaga caaagaaaag aagaagtaaa
     1021 ctagataaaa gtgtttttat aatttgtatt gaatatttaa gtctttgtat taaaattaaa
     1081 gaaaatttaa ttaaaaatgt ttggtatatt aacttataac agcagttaaa attgaaaaat
     1141 tataactgct gtttctttta ttaaaaatat ccaaatgtct gtaaaaatag actataatag
     1201 agtagtataa ataatataaa ggggataaat taaagtatgg aaaaagtgaa agttgctgtg
     1261 attggagcag gtaatagagg aacgtatgca tatgctccat atatttatga aaattctgat
     1321 gtatgtgaaa tagttgctgt agcagaacct aaaaaaggaa gacgagaact atttacaaaa
     1381 aaatataacc tagatagtaa aaatgtattt gaaactttag aagacttttt taaacatgac
     1441 aagatggcag atgcagtaat aatagctaca aatgatgata gacattatga tgtagcaaaa
     1501 ctagctcttg aaaaaggtta tcatgtacta ttagaaaagc caatgtctaa tagtttagat
     1561 ggtttggtac atatagatga cttgtgtgat aagtataaag ataagatttt tatgatttgc
     1621 catgtactga gatattctcc attttataat aaattaaaag aaattgtaga aagcaaaaaa
     1681 ttaggagagc tagttagtat acaatataat gaaaatatag gatattggca ttttgcacat
     1741 agctttacta gagggaattg gagaaatagc aatgaaacca gtcctttaat tttagcaaag
     1801 agttgtcatg acatggatat tttgttatat ttagtaggaa gtagatgcaa aaaaatatct
     1861 gcatttggaa gcttaaagca tcttaataac caaaatgcta gtggagagat ggcacaaaat
     1921 tgtcttcagt gtttagttga aaaaaaatgt ccatattcag caaagagaat ttatttagaa
     1981 aaagatagaa gtataaatag agctgttcac ataaatccaa ctgaagagaa tctattaaat
     2041 atacttaaga caagtccata tggaagatgt gtttacagat gtgataataa tgttgtagac
     2101 aatatggtaa atatactaca gtttgaaaat ggagtgacgg ctacttttaa tttatgtgca
     2161 tttactaaag aaaatggaag aactataaag ttaatgttta gccatggtga agttggtggg
     2221 gatttaaata aaaatgagat aagaattaaa gaatttggaa aaaacgaaga aatagttatg
     2281 aatccatcaa atcaacagaa tatggtagag gaatatgata gaaatcttat agctgaattt
     2341 attaaattag tttcaaataa agaactagaa aagggtagag tagcagcaaa agaagctata
     2401 caaagtcatg taatggcatt tgcagcagaa tattctaggg tcagtgatga agtagtgtat
     2461 atagaagaat tttttgatag tgctaagcaa atgactaagg agatagaaga aactatattt
     2521 taattaataa aaatttatta aataaaataa agatagatta aataatagaa atacgaaaat
     2581 atgtgttcta ttaaggaatc tatctttttt attaaggttt tttcttatcc ctatcaaaaa
     2641 aatccataca ttctgataaa agataagaaa gtaaagcgaa taagatttct gtacttaaag
     2701 gaatcgttac tgaatccata aaggtatcaa ttatgttcat agctagaaaa gttagtataa
     2761 aatcaattat aaattcgcaa agtttatata ttgagtatgg taatttgttt acatatctga
     2821 atatatctaa aatagtagtg catagaaaat caattggagt ggttatacaa aaatatatta
     2881 aaaagaaaat taatacagct ccaagagatt tatattcaaa tcctaagaat gataagaagc
     2941 caattcctat aaccgtgaaa atacaaaaag ttataccaaa aaaaacagca aaaaataaaa
     3001 atacagaaat aaattcgcct aaaggaatcc ttcttttatt acttatctta atcaactcct
     3061 taatttatga tatgaaattt atataaataa taacattctt aaaaattcat aatagtcctc
     3121 tgtttcaaat agtactgttt gagaatctat ttttttcata tttggataaa aagaggcctt
     3181 aaaagcttta taatgttctc taaattttat ttctatctta aatttatctg gcaattttat
     3241 taagtgccta gataaatctc cagataaagc ttttttgaca cctttttcaa ttttttttag
     3301 tgcaaggttt ggatggattg aaattgaacc atttccaatt ccttttgaca caggaacagt
     3361 gactatattt ggatttagtt tttttgcact ctcacaaagc atttcatctc cacttaaaaa
     3421 tgctacagga acattataat atgcagcaat ataagcattt atagtaaatt cagatgctat
     3481 ttcatcattt attttaaaat agtcataatc aatatccata gtatgagaaa gtggattacc
     3541 attacaagta gctccagagt gatagccggt gaatatacat gcatcaaagc tttcatcaat
     3601 tcctgccatc ataactagtg ggtctttggt ccatcctcta agtattttga cgttttcagg
     3661 taaaatattt ggattaatat ttctaccact gtcatgtgca tctttaacaa gtatttcatg
     3721 tacaccagat tgagtagctc catcacatgc tgctgttatc tctttactca tttgtgttct
     3781 atagtgttgc gaaaattcac attcaagttt tgtttcatcc caatttacaa taccacaagt
     3841 accttctata tcagcactta aatacaattt cataatatat acccccttat gtttattatt
     3901 gatgataata acagataaca gaaaatacat aatcaataaa gggcataaaa atattatgtc
     3961 ttactattaa ctattattaa atctgtaact gaaaaataac taaattggta taaaaaaata
     4021 taacactatg tgt
//
LOCUS       DAENCV010000225         4000 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAENCV010000225 DAENCV010000000
VERSION     DAENCV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.228"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     regulatory      5..102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            157..333
                     /locus_tag="KQD82_003730"
     CDS             157..333
                     /locus_tag="KQD82_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382167.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            559..759
                     /locus_tag="KQD82_003731"
     CDS             559..759
                     /locus_tag="KQD82_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG4382168.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            1695..2539
                     /locus_tag="KQD82_003732"
                     /pseudo
     CDS             1695..2539
                     /locus_tag="KQD82_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2763..2927)
                     /locus_tag="KQD82_003733"
     CDS             complement(2763..2927)
                     /locus_tag="KQD82_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382169.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(3468..3800)
                     /locus_tag="KQD82_003734"
     CDS             complement(3468..3800)
                     /locus_tag="KQD82_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG4382170.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT         1569 a          501 c          442 g         1488 t
ORIGIN      
        1 aataaaacta aatcggcaaa actagagaaa tttagtgacg caaagctata gggactaaga
       61 cttatataaa tatcttatga gttatgtcag ccagttgcca aagagatagt gttctttttg
      121 tttttatgaa agttttttag ggggataata atttatatgt ttggaaataa aatggataaa
      181 tgtacacatg tattgactgc ttatattagc agttcgtatg attattgtaa ttttttagat
      241 acacagttag atgattttat attagagtac ggagaaaatg tagtagagtc ttgtttacac
      301 caagtaatgg tattggtaag taagtataat tagatagaca ttgatttcta gaaaaccctt
      361 tttagtttta tctacattta tattaatata tagcaatgta aaacgtttaa atatattttt
      421 aatattaaga ataattaaaa tgttaactaa aaaatataat taagtagaca tttctattaa
      481 tctatgatac aatagtattt gtggtaatac acaattaaat caagtatatc ttgatatagg
      541 ttaataggag tatttttaat gaataatgga atagtgaaat ggtttaataa tgaaaaagga
      601 ttcggtttta tatcaatgga aggtagagat gatgtgtttg ctcatttctc agctatacaa
      661 acttcaggat ttaaatcatt agaagaaggt caacaagtaa gctttgatat agttaaaggt
      721 gctagaggtc ctcaagcaga aaatataact atattataat atatagattt ttcgggaaag
      781 actcttttta gagtcttttt ttattgttaa tatatgtaag ataatcattg gtgaagtaaa
      841 taaggtatat tgagtaatgt tcttgatagt ttatatgcta atgaatttca aaaaaataag
      901 cactcctata aaaagagtgc tcttaatgta aatataaacc ttcattggtc cagcctaata
      961 ccccgtattc tccaagttct aactattttt tatatacatt atcactttca tccatatcta
     1021 acacaattat ttcttcttta gaatcaattt gtaaaatatg aggactgtgt gttgtgacaa
     1081 ttatttgtgt ttttaggaga attgttttta atgtaataaa aattttacat accacatagt
     1141 tgatataaaa cgcccagatt ttctacaact tccatatttc taaacatttt tacataccac
     1201 atagttgata taaaacccaa aataaactta gcatctccaa tgtttacaca tatacaatgc
     1261 tcttaaaatt gcagtgaacg cgcaatagtg caattgataa tacttatcat atattctcaa
     1321 taccttatat ttcaactgct aaacctcaat ttatcacaaa tatcgctcac tgcaaaattt
     1381 ctatattttt attatatcat agcatataat taatattttc aattaatttt tacatttaat
     1441 tatgtttaaa ttctataata atgcaacttt aatgtttctt tctggaacta atataaaaac
     1501 tatttcagaa agattaggtc atgcagatat aaaaataact atgaatagct actctcagtt
     1561 tttagaagag atggacagag aagcatttga aaatttgagc aaaatactat ttattatatt
     1621 ttaaagaaga tgacttattc aatatttgat gctagaatat aattaataaa aatttaatta
     1681 taggtgattg tattatgaat attattaaaa gcataacttt actcttaaca ttattaattg
     1741 tgatattaat gacaggctgt tccccaagag aaagtgctag tgaagttgca aaaaaatata
     1801 ttgataagat gaactctggt gactatgaaa cagcatatag tttattaagt aaagatagta
     1861 aaaaaaaaat tagtatagaa aatcttgaag aatttcaaaa tattttagtt agtacaaaaa
     1921 gaataattag ttataaaata ggtaaagaaa aaatcataaa aaaatataaa catgatggaa
     1981 aagaatataa aaatgttgtt aaattagaag aaacttttaa tataaaaaac cttctttata
     2041 aaaaagatga ttctctaaaa gtaaatagat attttgtagt tgaaaataac aaatacaagc
     2101 tattattgta tgaaagtttc aaaaaagaat ttggtattaa tagtatggac cttgctcaac
     2161 ttaagcttaa tgattttgga ctaaaatact ttaaagaagt tgaccttata ctaaaaaagc
     2221 tattgctatt aatcctactg attcaaagct ttattatgcc caagcatgta attatatgaa
     2281 ttttgatgga tttcctggtt attcagatac aacatataat gctttagatt caattaataa
     2341 atctttaaag tatttagata aaaatgattc tgactacaaa aaaaatgcaa gtaatatata
     2401 taacttaaaa ggagttattc ttatgtttca caaaagattt gatgaagcac aacaatattt
     2461 aaataaagcc ttatacttta acaaagataa tgaagaccca aaatataatc taaatacaat
     2521 aaaaaaacat ctaaactaga tatttaattt ttgaatttat aaaatatttt tttatgtata
     2581 attcagcact gaatcataat ctctactaaa ctaattacaa gtttaaatta taaagactta
     2641 tttctatata aatattactc tataacaata agtttcttat ttaaatttta aaatattatc
     2701 taaaaatcat aaaaaagaag gtataaagag ctaccctcaa gctcaatata ccttataaat
     2761 atctaaactt cattttttac taatttttct atatctttga taatatgttc tttagatttt
     2821 tttccagcta tggcatcaaa aattgcaaca gcctttttct ctccaaactt ttctgctaca
     2881 tcattaaagt ctaaaaatct aacactttct tttggtttac caaacatata acacctctca
     2941 tacattttta tatatttatt ataacttcta tagacttatt tttcaccaat aaacgttaga
     3001 caaattagtc cctttatttt tttaaactag ctttaccttc aaaattctta acaaaaacag
     3061 tttattctac tctataaata aaaatagaca atttaatttg taggaaactg ataatagaat
     3121 acagtaaata aaactttgtt taatctatct gaaaaagtta tctattaaaa ctgctatgaa
     3181 tttataaaga atattctagt tcataaaaat acaaatatgt aaatataatc accttaatat
     3241 taatttattt aaaataaaaa atgtataaaa gatttaataa tgacaatata ctattctcaa
     3301 tacaatctat gatgtaaata atttaaaact actatattat ttaaaaattt atatttatat
     3361 atgcaattct aaaaacttaa taagaagtga acttagaaaa taaagcttaa tttcaaagac
     3421 ttaaatagac tattattatt agtctaaata aactacgtct atcaaaatta tatattaaat
     3481 tcttctaaaa tttttattgc aaatcttgcg tttggatatg taatagaccc gccaccaatt
     3541 acacctattt ttatagcttc tataatttca tctttacttg ctccagaatt aatacaaccc
     3601 tcaatatgat aacaaataca ctcatcacat cggcttaaaa ttgaaatagc tagtcccatt
     3661 aattccttat tttttttact tatagcccct tctgaataag tcttatcatc aagacttcca
     3721 aatactctaa aaaactcatc tgtttcgctt aattttttat tgtaaaatac cctatattct
     3781 ttcatttctc tagtattcat cttctacctc ctatgttttt cttataagaa tatcacactg
     3841 tacttatatt ttcaatatta aatacaaatt ttcattttat gttcattagc ctaatttata
     3901 ttattttttg aaatctcaat aagttatcat ataagtaaga ttttttcata actaaaatag
     3961 aaatcttagt aatttcttta ttacctatct cagttctgaa
//
LOCUS       DAENCV010000226         3922 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAENCV010000226 DAENCV010000000
VERSION     DAENCV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.229"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..601
                     /locus_tag="KQD82_003735"
     CDS             <1..601
                     /locus_tag="KQD82_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG4382171.1"
                     /translation="YLIIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYMF
                     NLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNIN
                     FGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIK
                     ALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            555..983
                     /gene="ytfJ"
                     /locus_tag="KQD82_003736"
     CDS             555..983
                     /gene="ytfJ"
                     /locus_tag="KQD82_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG4382172.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1231..1671
                     /locus_tag="KQD82_003737"
     CDS             1231..1671
                     /locus_tag="KQD82_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG4382173.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1687..3138
                     /locus_tag="KQD82_003738"
     CDS             1687..3138
                     /locus_tag="KQD82_003738"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG4382174.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3229..3897)
                     /locus_tag="KQD82_003739"
     CDS             complement(3229..3897)
                     /locus_tag="KQD82_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG4382175.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSITAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1639 a          354 c          671 g         1258 t
ORIGIN      
        1 gtatttaata atagctgtaa tttttattat aagtgtaact atgttgattt taatatctca
       61 tataaacatc ttaataatag ctgatataaa aaacaaagat atttgtttaa agcttaatat
      121 aaaatacatg tttaatttaa taaatataaa tagacaactt tatccagctg aaaatagtaa
      181 aaataatgat aaaaaagagg ggatgaagaa taatatagat tcaagtatat tattagctga
      241 tgaccttttg agcatttata gattattaaa aaagataaaa atacatgaac tttattcaaa
      301 tattaatttt ggaactggaa atattggctt aacatcttct gtatatgtat tgataaatac
      361 tttgtatgga aatttattta atatgattga tgcagaaaaa atgtatttaa atgtaaatcc
      421 agattttaca aaagattatg ttttaggaaa tataaggata catataaggc caagaataaa
      481 agctttattt aatattataa taatgattaa taaaattatg aataaaaata aaggaaataa
      541 ggaaggtgat agtaatgaga gcaacaggtt cgatacagag tcttatggaa acaactcttg
      601 aaacaattaa aggttctata gatgcaaata ctattatagg agaccctata aaaacagata
      661 ctacagtggt tgtacctata tcaaaggtta caattggctt tggtatagga ggaggagaat
      721 actcaaaagg atatgatgat aaagataggg aaattgaatt aaaaaatgaa aaatcagata
      781 cgaattttgc tggaggtagc gcaggggcta tatctgtaca accagtagca tttgtagtag
      841 ttgaaagtgg agagactaga ataatgagtt tagatagtaa tatcaatcta gtggataata
      901 tattatctat aactcctaga gtattggaga aaattcaaaa tatatcacaa agtaataaga
      961 atcaagataa aaacaatatg taagaataaa atatttttaa atatgtatat agttttaagt
     1021 aaagtagtgg ggttaatata aatcccacta ttttgttgtg tactttaaac atttatatct
     1081 aataagattt tagtttaaaa caatttaaat agataataaa gttgatatat agtataatta
     1141 taaaaagaaa agaagtatta tattatagat atataaacgc atttgagata taagagggag
     1201 ttataaatca ttagttttga ggtgaatgtt atgagcgata aagaattatc tattttgttg
     1261 ttagaaagta tgaataattt tcataattta ataaagatta ttaataacga aagatataaa
     1321 aaagataagg ttcttacaga aagacagttc tttgccttag taaaaataag aaaacatgat
     1381 aaaatagagc ttaaaaatct aagtagagat ttgcatgtat ctacttctag tctgtgtata
     1441 ctacttaata aattggttga gcaagagtat gtatataggg aagaagatag tagagataga
     1501 cgaaatactt tttatggaat aactaaaaat ggagaaaaga tattggataa tgaaatacta
     1561 aagtttgtat ctataataag tgataaaatg gattgcctag atatagataa taaagataaa
     1621 ttatttactt ctttggaaga gtctaaaaat ataatagaac aattatttta aaaaggagaa
     1681 tttattatga atataaagta ttctaaagag atattaaaaa gtttagaaaa tgctataaca
     1741 gtagtagctc tatttgaggg agaagatgtt tttacgacat ttgataaaat aaatatgata
     1801 agcagaaatt ttaattctaa agaaaatagt atagatactg taacattaat tgaaaataac
     1861 aatttggtaa atgtttgttt tgttggtttt ggtaagagag agttattgaa tagagaaaaa
     1921 attagaatca taggtgggaa tttgtgtaaa aaattaaaaa atatattgaa aaatgaagca
     1981 tataagagta gtgatgttaa tgtactgaat cttaatttaa atagtgatga aataggttgt
     2041 tttgtagaag ggatattact tggaaattat aagtttgata agtataagac aaaaagtaag
     2101 gattttgaaa gaaaagaagt tgatactata actatattta ctgaaaaaga agttgagtgt
     2161 caaatagaaa aggctaaaat attagctaat tcaactataa tagctagaaa tctagttaat
     2221 gaaccatcaa acgtaatata tccaaaaaca ttagcagtag aaacagttaa acttggtgca
     2281 gagtttgggt ttaatgttga tgtttatgaa gaagaaaaaa taaaggcatt gggaatggat
     2341 gcttttttcg ctgtttcaag aggttcaatt aataaaccaa gattcatagt aatgagatac
     2401 tttggagata aagaaagtac agatatatta ggtctagtag gtaaaggatt gacttatgat
     2461 actggtggat attctttaaa atcaaatgct tctatgctag atatgaagac agatatggct
     2521 ggtgctgcga gtgttatagg tgctatgtgt gctatatctc aaagtaaatt aaagaaaaat
     2581 gttatagcag tagtggctgc atgtgagaat gctttatcag gaggttcata caaacctgga
     2641 gatataatta gttctatggc aaagaaaact attgaagttt taaatacgga tgcagaaggt
     2701 agacttacat tagctgatgc aatatactac attataaaca atgaaaaagt aacaaaggtg
     2761 gttgatgtag ctacattaac tggtgctgct ttaacattgt taggtaatgt tgcaactcct
     2821 atagtaacaa ataatgatga tttttattgt gaattagaaa aagctgccac tttatcaggg
     2881 gaaagggtat ggaagatgcc aatttatgat gaatttaaag atatgataaa aggagaagaa
     2941 gcagacctta aaaacactgg tggtaaaaat gctggatgta taacagcagg agcttttatt
     3001 ggtgaatttg ttgggaatac tccttggata catatggata tagcggggac aagtacatca
     3061 tcaaaatcaa ttggatacaa agcaaagggg gctacaggag aacctgttag aacattgtac
     3121 tatttggcag aaatctaaaa aacaattata aaatataatt tataaagcca cgtggatatt
     3181 tttgtaattt aagattatct atgtggcttt attttttatt taagtaaatt atttactctt
     3241 tttctttttt tcaagtataa aagtatctct attaaataaa agagcaggta tgtacataat
     3301 actagtaaca aaaataaacg taaaaatagc agttatgcta tgacttaaaa aaccataata
     3361 gtacctagag ttggtaaatg tgtttagtaa aaatatgaat gaaaaataaa taaaacatat
     3421 agctaagtta tctctaagtg catatctgtt aactctaaat tttctaacat caaccatata
     3481 tatatctgca taaattatca agcacttaga gaagataaag ataaaatata ttaagctagg
     3541 aaattcatag cccaagtttt tagaaaaaat gagttctcca actgaacaaa ctaaactcca
     3601 agaaaaaaat atattaaaaa gatgatactg cttaaaaatt agtatggcta tacacagata
     3661 tatacaaata taattgattc caattggtaa agagtttaga gtactataat tacctgaaga
     3721 aataagtaat aattgctcta gagccataat tattacaagt aatgtacaaa ttatcttctc
     3781 aactgtatag ctataaggta aaatattttt ggttagtttt ggacaatagt ataaaaataa
     3841 tccaaatata aagagactta caaattgttc actagaaaaa agaaaaattt taaacatata
     3901 acattctctc ctataataaa tt
//
LOCUS       DAENCV010000227         3825 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.230, whole genome shotgun sequence.
ACCESSION   DAENCV010000227 DAENCV010000000
VERSION     DAENCV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.230"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            190..573
                     /locus_tag="KQD82_003740"
                     /pseudo
     CDS             190..573
                     /locus_tag="KQD82_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      929..1022
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1105..1590
                     /locus_tag="KQD82_003741"
     CDS             1105..1590
                     /locus_tag="KQD82_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG4382176.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            1946..2344
                     /locus_tag="KQD82_003742"
     CDS             1946..2344
                     /locus_tag="KQD82_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382177.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    2780..3043
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3194..3745
                     /locus_tag="KQD82_003743"
     CDS             3194..3745
                     /locus_tag="KQD82_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG4382178.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1674 a          331 c          567 g         1253 t
ORIGIN      
        1 gtttgaaagt taaataaatt tatagatatg aacattattt tacacttaaa aacagactaa
       61 acacgtagag tacctggtaa aaattaaata tagtttttgt tctaaaaagc tcctattata
      121 atttaagcta gataaatttg aagaagtgac ataagaagaa gtgacataaa ttgaaagaag
      181 gaatatatta tgctaaataa aaaattacca gatacagaat taaaaattat gaaatgcata
      241 tggaatactg gttataaaac tgtaatatca aaagatgttg cagatgaaat agaaaaaaca
      301 tatggttgga agcatacaac tacaattata cttttagcta gattaaccaa aaaaggtttt
      361 ttaatatcat agagaattgg gaggcatgta cactatacag tattaataaa agagagagaa
      421 tatctcaaaa tagagggaaa aagagttttt agaggtttac ataacaatcc tttatcagaa
      481 ttaatttcaa aattacatga tggagaagaa ataactgaag aaaaaataat agaaatagga
      541 agttggataa aaagctggaa atataaagat taataaataa aaggtagcta ataatataag
      601 tgctaccttt tttatgtgga taatattggt gataagttgt ggaaaactta tataaaaatt
      661 gtggataaag tgttttgctg acattttact gacaaaagtt aaaaaaataa atgttatata
      721 tattgtaaaa tgttgaaaaa tagccatata tataaaatat aagtaacact atagataaat
      781 tactgtcttt tatattgact ataaagaaca gtaattgaat aaatataaag atattttttg
      841 attattatta attaaaaatt taatttatat ttaaataata taaaatttga ggtaatatta
      901 ttttttaatt gttcaaatta tgttattatt cagatatagg caatattaga taaatctaat
      961 gacgcaaaac tatagggact aaaatcttta tgtaataaag gtcatgtcag ccagttgcca
     1021 aaaagataat aattcttttt gttaaaagta tttttagagt tatatatttt aataacttaa
     1081 tatttggcaa gaggagggct taagatgata tctgaattaa tgttaaaaaa ttttcgcaaa
     1141 tctagcatca attttaagac taaaattaaa ttgaatttaa gagaaaaacc agacataaat
     1201 agtttgaaat taaaaagtat accagaaggt aaaattgtta agttaaagtg tgtagatgga
     1261 atatgggcag aagttgagag taattatgac aaaggttggt tactttataa atatttagaa
     1321 agattaagta atgctaaaaa caatagtaat gatacaataa aaaatagaaa aaagaattac
     1381 ataggaaata taagaacaaa tggattaagt ttagaactta gaaatgatag aacattagaa
     1441 tctaaagtta taactacaat ccctgatggt tttaaagttg aagtatgtta tagcgttgga
     1501 aaatgggcaa gagttaatat caataaaaat gaaaaacgat attctggata tgtatataat
     1561 caatacatag aagagcattt aagtttataa tacgatacta gcaagcttta aatatattaa
     1621 taaatcacaa aaaagaatcc cattaatggg attctttttt gtagaaacat tataacagaa
     1681 ctaaagagaa taaattaact aatttatttt tattataaag taattataaa tggtaaattt
     1741 aattgaaagt agatttaact tatactttat acaccaagta tcataaatta atactacttt
     1801 gaaaaatctt aaaaaaagta aaagttatga taataaaaat aatattaaag ataataagat
     1861 atataagaaa cttgaaaaaa tttgtaattt ttagttatat atatattata atgaactagt
     1921 taaaaatttt tatgaggggg aaaatatgaa taagaagaaa aaattgattg ggatagctat
     1981 atcttttcta cttataatca gcattataaa tccattacaa gtagaagcag aagctattat
     2041 tgagtctagt gtatacattt cttcaataaa agagaattat acatatgata atactaaatt
     2101 taaaactatt aaatctaaaa gtagacataa aagtaggaaa tataataaaa gtaaagtaaa
     2161 gttgaaaaaa cgaagtataa gaactaaaaa gtatggatat caaactataa ataaatataa
     2221 aagacaaaac gcaagaagac ataatatcct aacaaaatta atattgtcca taatagtcat
     2281 atttttggtt atagttttag tagtgctaat atttattaaa agaaagaaga aaaaatttta
     2341 ctaaaaataa ttaaaagaat atcatttttt taatcattgg tatctattta ataatatgat
     2401 gtaattaaaa tggagtaata ttctgtttca aaggaggcat ataaatacag gaatgagtat
     2461 taaatgatgg ttaattaagt aaatttattg ataaatgtac ttaattaact ttgaagaatt
     2521 taattcctca tgaatcaaaa tataaaatta catcaacagt tattcacaaa attaatataa
     2581 gaagaattag agccaaagag cagactatta gaccattaaa gtaattattc atagaatttt
     2641 gtatatgaaa agaaatgtat agtataattt acttttttta taaaaaatag attacaaaat
     2701 atatttttta taaaaaggta ttgacatttt ttagaaaaaa gaatatcatt attttaacaa
     2761 caaaattcaa tacaataaat taaatcctat gacaaggaag agtagttaaa tttaggttca
     2821 gtatagagag ctgaggatgg tggaatctta gcaagaaaaa atttgatgaa tggcccttag
     2881 aggaataaat tgaaatcata gttttgagag tagactttat cgtcagtcgc acgttatagc
     2941 gaataaggta tgtaagtacc tgataatgag atgtatgaaa attgaatttt atattaattt
     3001 ttatataatt taggtggcaa cgcggataat ctccgtccta ttagtttagg acggagtttt
     3061 ttattttact gaaaatttaa aagatagaag gtggtgagaa agatgttatg aatttcaaaa
     3121 gctaaagatg atgatatagt aggttggcaa aaaaataaat tgaaaattaa aaaaatagga
     3181 ggaagtacaa aaaatgaaaa caaatacaaa aaaattaact ttaaatgcaa tacttttagc
     3241 gatgggatta ttaatacatc aaatcacacc agctatagga ttaccaatga agccagatgt
     3301 accactagca atgttatttg taatattggt tttaaataga gatgattata aaacttgttt
     3361 gatagcgggt atagtaacag gaatatttac agcacttaca tcaagttttc cgggaggtca
     3421 aattcctaat gtaatagata aaactcttac agcaaatata gtatttttat taatgaatat
     3481 tagttataat atgcctttta taaaaaaatt agctaagaaa acacaagata ctgtagtagt
     3541 tgcaataata atgccaatag gaactcttgt aagtggaact atctttttat tagcagctca
     3601 agctatagtt ggtcttccag gggcttcatt tacagcttta ttcttagcag tagtattacc
     3661 agctgtttta ataaatctag ttgcaggaat atttttattc aagattgtta gtctatcaat
     3721 aagaagagtt agttatcaag cttaaacacg aaataatatt ttactttata aagcattata
     3781 ataactaaat aacataagaa taaagtagat tataactatt ttatt
//
LOCUS       DAENCV010000228         3810 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.231, whole genome shotgun sequence.
ACCESSION   DAENCV010000228 DAENCV010000000
VERSION     DAENCV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.231"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(220..585)
                     /gene="rplL"
                     /locus_tag="KQD82_003744"
     CDS             complement(220..585)
                     /gene="rplL"
                     /locus_tag="KQD82_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG4382179.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(643..1149)
                     /locus_tag="KQD82_003745"
     CDS             complement(643..1149)
                     /locus_tag="KQD82_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG4382180.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(1192..1320)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1372..2070)
                     /gene="rplA"
                     /locus_tag="KQD82_003746"
     CDS             complement(1372..2070)
                     /gene="rplA"
                     /locus_tag="KQD82_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG4382181.1"
                     /translation="MAKKGKRYAGALEKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(2141..2566)
                     /gene="rplK"
                     /locus_tag="KQD82_003747"
     CDS             complement(2141..2566)
                     /gene="rplK"
                     /locus_tag="KQD82_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG4382182.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(2605..3147)
                     /gene="nusG"
                     /locus_tag="KQD82_003748"
     CDS             complement(2605..3147)
                     /gene="nusG"
                     /locus_tag="KQD82_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG4382183.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(3167..3388)
                     /gene="secE"
                     /locus_tag="KQD82_003749"
     CDS             complement(3167..3388)
                     /gene="secE"
                     /locus_tag="KQD82_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG4382184.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(3414..3563)
                     /gene="rpmG"
                     /locus_tag="KQD82_003750"
     CDS             complement(3414..3563)
                     /gene="rpmG"
                     /locus_tag="KQD82_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG4382185.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT         1064 a          753 c          452 g         1541 t
ORIGIN      
        1 tatatacttt atatacagac attacaacaa ttgtttatat tttaatttga taataatgtt
       61 aaattaaaat accattacac catatatgat acccttaatt tagtactatt atataaattt
      121 ttttatttta taaaaatact ttctttataa ttccaaatac tttctttata gttttttata
      181 aataaaaaaa gatacccaat gggtatcttt ttaaagaaac tacttaactt ctactttagc
      241 tccagcagct tctaatttct cttttatttg atcagcttct tctttagaag ctccttcttt
      301 aactgtctta ggagcattgt caactacttc tttagcttct tttaatccta atcctgttat
      361 ttctctaact gcttttataa ctccaacttt tgaagaacca acgtcagtta atactacatc
      421 aaactcagtt ttttcttcag cagctggtcc accagcagct gcaccagcta ccattactgg
      481 agctgaagct gatacaccga atttttcttc agcagcttta actaattcat ttaattctaa
      541 aactttcata ttttctatag cttctaatat ttgttctatt gtcattttta gcacctccga
      601 attttatttc aaattaatat ttataatttt ttagttaagc aattaagctt cttcttgacc
      661 ttctgccttt ttcgctattg catctattaa gtatgcgaag tttgatactg gagcttttaa
      721 gctaccaagt aatttagcga taagaacttc tcttgatggt atgttagcca tttcaacaat
      781 cttagattca tcatagaatg caccttccac aatacccatc tttaatttca tttttggatg
      841 agaatccatg aatcctttta atattctagc tggagcaact ggatcatcat atccaaatgc
      901 tatggcatta gttccaacta ataattcatc attaaattgc tctataccaa cttctttagc
      961 tgctcttcta actaaagtat ttttgtatac tttgtaatct acaccagctt ctctcatttg
     1021 ttttcttaac tcagttactt cttcaactgt taatcctttg taatctacaa caacagcagc
     1081 agaagatttt tgtaactttt caactatctc agaaacaact tctgatttaa tttctatagc
     1141 ttttctcata tagatgtgca cctccttcac caaataggct tagtatttac cgcaacatta
     1201 taaaaagctt ttgttgccaa aagtagacat caaaagcttt agtaatctca atttatctaa
     1261 aacctcggcg ggatattaag ctttcgcacc cactatctac ggcaaatcta ttctttttta
     1321 cattgacaag tgatataata acatttttgt caacctgtgt caacagtttt attattcagc
     1381 tgttcttgct gggtttattt taactccagg tcccattgaa gaagctactg ttatacttct
     1441 taaatattgt cctttagcag cagctggttt agcttttatt atagcatcca ttaatgcagt
     1501 aaagttttca gttaattttt ctccaccaaa tgatactttt cctactggaa cgtgtattat
     1561 gttagttttg tctaatctgt attcaacttt accagctttt atttcatcta tagctttagc
     1621 tacatcaaat gtaactgttc ctgatttagg gtttggcatt aaacctttag gtcctaatac
     1681 tctacctaat ctacctacta cacccatcat atctggagta gcaactacta tatcaaagtc
     1741 aaaccagttt tcaccttgta ttttttgaac taattcttca gctcctacaa agtcagcacc
     1801 agcttgttca gcttctttag ctttttcacc tttagcaaaa actaaaactc tttttgtttt
     1861 accagttcca tgaggtaata caactgcacc tcttacttgt tggtcagcat gtcttgagtc
     1921 aacacctaat ttaatgtgtg cttctactgt ctcatcaaat ttagctccag ctatatctga
     1981 aactaatgtt aatgcttctg atgcatcata aaatttagtt ctatctactt tttctaatgc
     2041 acctgcatat cttttacctt ttttagccat tttattttcc tcctcgtggt taatttcggc
     2101 ttctagcctc ccactttata aaaaaaagtg tatcttttaa ttagtcttcg attacaacac
     2161 ccatgcttct agcagtacca gctatcatac tcatagcagc ttctactgat gcagcattta
     2221 agtcaggcat ctttaattca gctatttctc ttacttgagc tgaagtcatt ttagcaactt
     2281 ttttcttgtt tggttctcca gaacctgatt ttaagtttaa tgctttttta attaaaactg
     2341 cagctggtgg agtttttgtt ataaaactga atgatctatc ttgatataca gttataacaa
     2401 ctggtattat cattccagct tgatctgcag ttttagcatt aaattctttt gtgaatccca
     2461 ttatattaac accatgttgt cctaatgctg gtccaactgg tggcgctgga gtagcctttc
     2521 ctgcaggtat ttgtaatttt atttgaccta taactttttt agccattttg agcgcacctc
     2581 ctcagatttt tctataatat agtattagat tttttctata ccttcaagct ctattacaaa
     2641 tagagtcctt ttaccaaatg catttatgca tactttgact tctctatttt ctaagtcaat
     2701 ttcttctatg ttacctattt gtccactaaa tggtccttga gatactttta ctgtatctcc
     2761 tatttcaaaa tcaatatcgc tatttactac tttaggatct gttgtgtcta tccccatagc
     2821 tttgacttcg tcttcactta atggcactgg ttttgaacca ggtccaacga aacctgtaac
     2881 tcccttagta tttcttacta cataccaaga ttcatcagtt attatcattt taaccaaaac
     2941 ataacttgga taaactttac gttgtctagt tttttctttt ccagttttag tagtttccac
     3001 tacttcttct gttggaacaa ctacttgtct tatgcagtct tccatacctc ttgttttaac
     3061 tgctttctca attgtagctt taactttatt ttcatgtccc gaataggtat gaactacata
     3121 ccaacttgct tcttgtaatt ctgacattta acttacctct tttcgtctat tttagtaata
     3181 acgctagtgc accagataat atagtgtcta taccccatac taatatagtg catgaaataa
     3241 ctacagttaa aactatactt gtgtttttaa acagttcctt ttttgtcggc catgtaactc
     3301 tttttaattc ttgtttagtt tcttttaagt atccaaacaa actaaacctt tttttagttt
     3361 ttgcaccgtc atttttttgg gcagccattc catactcaca tccttataaa aaactatttt
     3421 gtttctttat gagttgtatg ctttttacag aatctacaat atttttgtaa ttctattctg
     3481 tctggattgt ttttcttatt cttagtagtg ttgtagtttc tttgcttaca ctctgtacat
     3541 gctaaagtta ctttaactct catctgatac acctccaact ttgtaataca ataatatata
     3601 ttgttaatat atgtgaatat aatatcttta aaatatgtat ttattaaaaa tctacagtat
     3661 tcaagatatt attaacttcc tataatcatt accttgtata agttatcaca aaatcctatg
     3721 ctgtgtcaat gtttttgtat ttctatgtta aaaaaagagg agcataaccc ctctttttgt
     3781 tattcctttt aatccatcag tttagtaaag
//
LOCUS       DAENCV010000229         3798 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.232, whole genome shotgun sequence.
ACCESSION   DAENCV010000229 DAENCV010000000
VERSION     DAENCV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.232"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            71..1183
                     /locus_tag="KQD82_003751"
     CDS             71..1183
                     /locus_tag="KQD82_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG4382186.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1391..1867)
                     /locus_tag="KQD82_003752"
     CDS             complement(1391..1867)
                     /locus_tag="KQD82_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382187.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2066..3691)
                     /locus_tag="KQD82_003753"
     CDS             complement(2066..3691)
                     /locus_tag="KQD82_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4382188.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1320 a          610 c          421 g         1447 t
ORIGIN      
        1 tatttcaaaa ttattttgtg atatatatat gttataataa atgtataaaa ttttgattgg
       61 tgggttatat atgaatgcat ttattagaaa aagaaataaa aactatgttg tttacctgga
      121 atttgaagat gaaaagacag gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa
      181 aagggaagca agtaaaagac taaatgaggt aaaagaaagc ttatataatg atggtttttt
      241 agtaccaagc gaaattacag tgtcagaatt tctattagat tttctaaata aatatagtga
      301 aaatatatct ttagctacat ataataatta tgttagtatt tgtaagaatt acattaatcc
      361 atctattgga aaatataaaa tacaagactt acatccagtt cacatacaaa actacataga
      421 taaactatct tataaactaa atcctcaatc aattaagatt catataaata tattgaaact
      481 tgcaataaaa agagcttata gactcaaatt aataaaagaa aatgttatgg atagtataga
      541 ggctccaaga tataaaaaat ttaaaaatga gatttatgat agagaacaaa tgataaaatt
      601 attagattta gcaaaaaata cagaaatgga attacctatt aatttggcta ttggattagg
      661 tcttagaatt tcagaagttt taggtctaac ttgggataat attgattttg aagaaaatac
      721 aataacagta aataagataa cttcccgaat taatggttct gttattctca aagagccaaa
      781 gacagaaagc tctgttagaa aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga
      841 atataaaata aaacagaata aaaatttatt aaaatcttcc attagaaata ataataattt
      901 gttgtttttc aataagaaat gtgaacctat tgctgaagat gttatgagta aaaaatttaa
      961 aagattttta gaaaagaatg aattacctca tattagattc catgatttaa gacactcaca
     1021 tgttacttta cttataaatt ctaaagtacc tataaaggtt atatccgaga gagtaggaca
     1081 ctcaaatata agtactactc taagcgttta ttctcatgta cttaaagaaa tggataaaga
     1141 ggcttctgac aaaatttctg aaagtttatt taatgctaat taatattaat ttatcttagc
     1201 tttaaattag cttagcttat tgagatagat tcattaatat taaatgtatc tatctcaaat
     1261 taataactac ttaaataatc tgttttattt taatttatcg cctcagttat tatacttttt
     1321 tacgaaaaat tatactttac aataaaaaag atattccctt atagaaaata tctttttaaa
     1381 tactatatta ttatatactt gctaacattg gaatatctaa catcttcttc attacatcat
     1441 ctactttttt tgctttatat tcattgtatc caattaaata tcctttttca tatgaacttt
     1501 ttaaaatctc tttttcatat aaattatcag atttggcttc tgtgacactt aaattataaa
     1561 gccctttaca cccatcttca taacctttat tatacccttt ttctgcaaaa tagtttttat
     1621 gatttttttc gttatgtact gaagatattg ttactccaga attttctttt gtactagcat
     1681 aaggacaaaa acctttattg tgttgatgtg ctgaatgtcc tccacaataa taacgtgtta
     1741 actctgattt atttttatta tgatgacctc ctgatgaatt atgccctcct gagtgagcat
     1801 atgaaaaatt ccctacacta atcatagcta aactaattac tacagctact aaccttttct
     1861 taaccataat ctattccccc caaaatgtta tatattttta ttatatcatc tactatatta
     1921 tatttaaatt cttaatatac aaaatattac tctattctgc taataaacat gtttaaacaa
     1981 cataaaaaac ctcctaatat ttgattaaaa atttacacac tcatgcatag catgtctcaa
     2041 atattaaaag gttttatact aataattatt tatcttttcc atacatttta gctaattggt
     2101 cttgtatctc tttactttct aaatactcat caaagtccat ttttctatcc attattccat
     2161 ttggagttat ctctataatt ctatttgcta tagtagatat aaattcatgg tcatgagaag
     2221 taaataaaag cactcctggg aacttttcaa gtcctttatt tacagaagtt atactttcta
     2281 ggtctaagtg gttagtaggg tcatctaata caagtacatt tgcatttgaa agcattagct
     2341 tactaagcat acatctaacc ttttctcctc cagatataac ttgagcttct tttaatgctt
     2401 cttcacctga gaataacatt ctacctaaaa atcctcttat aaaactttca cttttttctt
     2461 cagagaactg tctaagccag tcaaccaaag aatattctac tccgtcaaaa aacttattat
     2521 gattttttgg aagataatct tgagaagtag ttactcccca tttatactct ccactgtctg
     2581 gttcaagttc tcccataact atgttgaaaa gtgcagtagt agctatttca tctcccataa
     2641 atactacttt atcatctcta tccaatctaa atgacacatt atcaagaacc ttaaccccat
     2701 ctatagtttt agttaaattg tgtacttcta aaacttcatt cccaatttct cttgcaggtg
     2761 taaatcctac atatggatat cttcttcttg atggttgtat atcttcaact tctaatttct
     2821 ctaactgttt ctttcttgat gttgcttgtt tagcctttga agcattagaa ctaaatctag
     2881 ctataaattc ctttaattga gctatctttt cttcagtctt tttattttgg tccttagcca
     2941 attgtaaagc caattgactt gactcatacc agaaatcata gtttccaacg tacatttgta
     3001 ttttaccaaa atctacgtca actatattag tacatatttg atttaggaaa tgtctatcat
     3061 gtgatactat tataactata gattcttcta aatccattat aaaattattt aaccagttta
     3121 ttgatttaaa atctaagtgg tttgtaggtt cgtccattaa tagtatttca ggcttaccaa
     3181 aaagtgattg tgctaacaat accttaacct tttccccacc aactagttcc ttcatttgtt
     3241 tgtaatgcat atctttcgtt atgccaaggc ccataagtat tttctcagca ttagtttctg
     3301 catcccatcc gtcaagctct gcaaactcac cttcaagttc ggcagctttt atcccatctt
     3361 cttcattaaa gtcttccttc atatacaatg catctttttc tttcattatg ttccaaagtc
     3421 tctcatgacc tcttataaca acatttaaaa cagtttcttc ttcatattca aagtggtcct
     3481 gttttaaaac tgacattctt tctttatcag ttatagatat actacctgta ttaggctcta
     3541 tttctcctga taatattttt aaaaaagtag attttcccgc accattagct cctattattc
     3601 catagcaatt acctttagtg aattttaagt taacatcttt aaatagttcc ttatcaccaa
     3661 atctaagtcc aacacctgta acttgtaaca ttaatatatt ttcctttctt tttgctcata
     3721 atttatttta tcataaaact ttgataacat ttttattata tattatcacg taaatagtat
     3781 attttttata ctaaattt
//
LOCUS       DAENCV010000230         3766 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAENCV010000230 DAENCV010000000
VERSION     DAENCV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.233"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(91..378)
                     /locus_tag="KQD82_003754"
     CDS             complement(91..378)
                     /locus_tag="KQD82_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382189.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(398..673)
                     /locus_tag="KQD82_003755"
     CDS             complement(398..673)
                     /locus_tag="KQD82_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382190.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(1310..1489)
                     /locus_tag="KQD82_003756"
     CDS             complement(1310..1489)
                     /locus_tag="KQD82_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382191.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1774..3240)
                     /locus_tag="KQD82_003757"
     CDS             complement(1774..3240)
                     /locus_tag="KQD82_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4382192.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         1333 a          599 c          371 g         1463 t
ORIGIN      
        1 agttcaacta tactggttta aataggtttc ctttagttta aataattcac ctatacctac
       61 ttgaatagtt cacttttatt tgcttaaatt ctataccaat actggcttaa acattatact
      121 accatgattg tattgtctat cttcaactac aataaaaaca ggttctaaat ctttagtaga
      181 tgttattttt aattctaaaa aaacattatc agttggtaat atttttacat cataattatt
      241 taaattatta ttcgtgatac tattaaatac atcaccttga cgttcaaaaa aaacattatc
      301 aactattaca aaataaacat cttcttttct actattttca acctctatag tattttgttt
      361 agttacattt agtctcatct tccacctcct attaatatta cttaattata ttaatacttg
      421 taattgttgc attatgttca tcacaaggaa gaggtttaaa aacaactatc tctgctaaat
      481 aattctgtgg aggatttgta actgaagtag cattgcaaat aaatttaaaa tttgttcctg
      541 ctacaacttg agtagaaaca ataagtggtt cataaccaac cccttctaac attcctacag
      601 cttcattaaa tacttttaaa tcttcttctt cgacagctct gaattttgac catccaccaa
      661 caaaaagact catataaaaa cctcctttat atatatattt atttatatat atagtagtac
      721 caattttctc aatattcaat attttctgtg taactggtca atgaaaatgc ataactggat
      781 aaaaatactt ataaaataac ttaactttac atataaatag cttactttac atgtaataag
      841 attattctac aatatgaaat gcatcttgtt ttgaatatat tctaaataca ataatggata
      901 ttttcaatta ttataattag aaaatatcca ttacttaact tgttttattt aaaatcacat
      961 agagacttta ttattttttt tattgcatga atagctattt caggtatcat aagttgatat
     1021 accccctcct tgaaaccaca aaaaacataa caaactttac gaatcttgtc ttaaaagacc
     1081 acaggtacat tccttatttg tggattggag tagccataac aagcaaattt tacaattttt
     1141 atgatgacaa aaatcaataa aatcgcttag tatataacac gaaacactac ttgttactat
     1201 taagaatagt tagataggaa gtatgcatta taccaataaa ggaatcaata aagaaagtta
     1261 aaacatccct acaaatcaaa aatctgttac atttttctat atttaaacgt tatttactta
     1321 caattggaga aataacaggt tttccttctc ggtcaagtaa aggcgtaaat ccggctgcat
     1381 ggccataata gtggaataca tagttcacac ctgtttcttt atccacccaa atctccgttg
     1441 catttatttt tccttgtgaa tatacttttt caaatctatc ttttcccatt ttataatcct
     1501 ccatattttt gattggtcta tgccttcttc acttaagtag aaaaaataca taacttattt
     1561 ttttaattct aatatgagca cgtaaaaatg tcaaacagct tatttaattt cataacttca
     1621 tgttactgtt cgtaaccttg tctgtttact tacactgcat tacagcacca ttttatcctt
     1681 tacattattg tattgcacac agtaaaaatg cttactttat gggatttttc aactaaaaaa
     1741 aggattttat ctccacaaaa catttactat ttgttacaat atacctaatt ttaatctcat
     1801 atcaacattt aatgaagata taaccttttt caaatattgt aaagactcct taaaaatatt
     1861 agaaaaagat tctgcaaatc tctcaacatc tccttttttt aaatacctaa gtatctcaaa
     1921 acatacatca aaacgtgttt tcaaaatttc attatattct tcatgaaaaa atctctctaa
     1981 atagattcca aatatcatat attgttttaa atgcaaaaat gtttcctgta atacataaat
     2041 ttgtgtataa tctactatct tacaaagtaa aacaaaatct ggataaacct tacactcttc
     2101 acaattattt tgtaagtacc ttaaatgagt ttctatttta tctacagcta tcaaatccat
     2161 tttatttcct agtaaaagta ctacatcctc aagagtaatc gtaacaaact ccatagcatc
     2221 caaaagcaaa ctcaaccttt tagctccttc ttctgtattt atataatcat catcatattc
     2281 agaaaaaagt gttacatatg ttcccttgcc attaattgtt ttagcaattc ccaattcatt
     2341 tagctcatta taggcacttc taactgttgg aagagataca ttatatctat taattatcga
     2401 agtaattgat ggtagacaat ctcctatttt aatattgcct tcaaaaattt gacaaattac
     2461 atctgaaaca attaagtcat ataaataaca tttttccaac gttacagaat taaaactact
     2521 tgtatctata ctttgtggag cacgattttc taccatttta agtatggatt ctctacaagc
     2581 tttatacata tatgttaata gttttttaaa ttcaattaga ttcccgtcca atccatataa
     2641 gtaagttttt cttacctgct ctatgtattt attacgaaaa aagtctatac aacaattatc
     2701 caagttatcg aaattatagg ccaattgtga aataatgaca tcatgctgaa taatctcaca
     2761 aaaatggtga atcaggaaat tatttgtttt acttattagt ttatttatat attttagcct
     2821 ttgttcttta aagactaaat aattagaact atttttttcc atacaatcga taatctcgtt
     2881 taattcttca aagtctttag atgtaaatct tttaatattg tacatagcaa tagaaggaaa
     2941 tattaaacta aaaatcttat atatttctga tatttgtaaa atcttctcat ataataagtc
     3001 agattctatt gattgtttac tcgtactctg gattaaaact ggtctagaac cagcttttgt
     3061 ctctataaag cccttttctg agagcatttt tattacagtt tttatagtat tttttgatac
     3121 catatattct tctgacatgc tttttataga atcaagccta tctccaatag taattttacc
     3181 agtataaatt tgttgaatta tatgctcaaa aataattaaa tattgtggtt ttctcttcat
     3241 atttttcctc ttcgataaat tagatttatt gttttttgta aggtatattt acttttattt
     3301 aatagggcaa aatagttgct tattttttcc tcataatcta taattaatat aaaccattta
     3361 tatatatggt ctaagtttta taatactgta atgcagtaaa atagcatatt gtagctatac
     3421 tacagcaaat taacatacca ttgtagtata ttaattggaa taattttaag tacttcaaaa
     3481 ttccagcaaa acatatatgt gtttttgata ttatgtatat tcgttaatag aatagctata
     3541 cttgttttac ataataaatt atatgatact tgctatatgt aattactata tttaatacac
     3601 ataactatta tacttactat atgtaattac tatatttgat acacataatt attatactta
     3661 ctatatataa ttactatact tggacacata actattatac ttgctatatg taattactat
     3721 actttataca catagttatc atacatgcta tatataatta ctacac
//
LOCUS       DAENCV010000231         3538 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAENCV010000231 DAENCV010000000
VERSION     DAENCV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.234"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(132..887)
                     /locus_tag="KQD82_003758"
     CDS             complement(132..887)
                     /locus_tag="KQD82_003758"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG4382193.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1327..2316
                     /locus_tag="KQD82_003759"
     CDS             1327..2316
                     /locus_tag="KQD82_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG4382194.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLRMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLIVLCGNKDAQPVVNSSSIY"
     gene            complement(2768..3484)
                     /gene="dapD"
                     /locus_tag="KQD82_003760"
     CDS             complement(2768..3484)
                     /gene="dapD"
                     /locus_tag="KQD82_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG4382195.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYDDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
BASE COUNT         1272 a          488 c          494 g         1284 t
ORIGIN      
        1 aagaaaaatg attatattta aaaggtaagc ataaaattta taaactttta gaatagaaat
       61 ctcaatcaat tagatattca aattgatgaa tatgaatacc taaatagatt gagatttatt
      121 atgtacaaaa cttatcctag tatatctgac attttataaa gtccagattg cttaccaatt
      181 aaaaatttag ctgcaactat agaaccgttt gcaaatactt catcagattg agaatgatgg
      241 cttaatgtaa gaacttcatt atcacctgca aatataacat catggtcgct gattatattg
      301 ccacctctta tagagtgtat tccaatttca tttggttttc ttttacaatc tctaccatat
      361 ctaccatgtg tattttcact ttcaggcaag acttctctaa ttgcattagc tatcatagta
      421 gcagttccac taggggcatc ttcttttcta ttgtgatgtt tttctatgat ttcaatatca
      481 aaaccttcta acatctttga agcatcctta actaatttta ttaatatatt tactccaaga
      541 gatgtatttg atgataataa tataggaatg ttttttgatg cttcttctat tttatttaag
      601 tcattttcgt catatccagt agtagctaat actacaggtg tattattttt tagagcatat
      661 gacaatattg gctctaaagt ctcatgatgt gaaaagtcta ttatgacatc agttttttca
      721 gttacatcag tcattgtttt gtaaagcttt gtatcactat gatgaccttt ttcatcagca
      781 gacctagcta caccacaaac caactctaat tcattatctt tttttacaca ttctgctaaa
      841 actcttccca ttgttccatt atagccacta attataactt tcaacataaa ttcctcctat
      901 gaaattaata aaaatttgtt atatttctat aagtttattt tatcactaat ataatttata
      961 taaaataata ttagtgataa taagcttaca atttatctta cattaaaaaa tttaagtgga
     1021 tttttcaaaa tatagataaa tttctttttc attgtttaaa tgataaaaat atatttgact
     1081 tcataccaaa aagagtgccc catgaactaa aaagttaatt ttgaaatacc atattcatat
     1141 atcatatgat ttatttggaa atcaatatta atacattaaa ataactaaat acatgtatgc
     1201 ataaagatgt ataaaaaata tataaacata tagtattaat tttaaattca acaatttaat
     1261 tgttaaaagt gttattatta aattagagat atcttaactt aatatactga gatgggagtg
     1321 atagatatgt taaaagaaga aaaaacttat caagagaaag acgcaaaagt attcaatgga
     1381 gtggcagctt tgataatgat tttattattt ttagtagttt caataggtat catggttttt
     1441 gggatagtaa aacttgatag tggatttact ggaataggtt cacttatgtt aattttggga
     1501 cttgtattta tagtagtaga ttttatccta ttttttgggc taagaatgat taatcctaaa
     1561 gaagctatag ttctagtttt gtttggtaac tattatggaa ctataaaaaa agaagggtac
     1621 tattgggtga atccattttg ttcagcaata aacccagcag tatcaagaat taatgtatcg
     1681 aaatcttcat ctgatgaaaa ggtagatata tcaactaatt caaggggcaa aaaagtatct
     1741 ttaaaaacaa tgactcttaa taatgaaaag cagaaagtca atgatttatt gggaaatcca
     1801 attataatag gagtggttgt aatttggaag gttatagatg caacaaaggc agtatttaat
     1861 gtggataatt ataatacatt cttatctatt cagtgtgatt ctactattcg caatgtttca
     1921 agactttatc catatgatgt atctgaagat ggagatgaaa aatctcttcg tggtagtagt
     1981 caggaaatag cagatagatt aaaagatgaa ttacaatcaa gggttgacat tgcaggaatt
     2041 gaagtgtgtg aagtgagaat tactcatctt tcttatgcac cagagattgc agcagcaatg
     2101 ttacaacgtc aacaagcaga ggctataata gcagcacgta aaaagattgt tgagggggct
     2161 gtaagtatgg tagagatggc attgactaat ttaagtgaaa ataatgttgt gactttggac
     2221 gaagaacgta aagcagctat ggtaagtaat ctgttaattg tgctttgtgg caataaagat
     2281 gcacaaccag tagtaaacag cagttctatt tattaaatta taaaaagttt aatagtttat
     2341 tttattgata tatagaaaat ttagtagttc attttattaa attatagaaa gattaatggt
     2401 tcgctttatt gaattataaa aagtttaata atttattcta ttgatatata gaaaatttag
     2461 tagttcattt tattaaatta tagaaagatt aatggttttc actttattaa attataaaaa
     2521 gtttaatagt ttattacatt gatatattga aaaattgaag gaaataaaaa tacttgtcag
     2581 caagaaataa aaatgatata agaatgaata agagaattgt aaaaaaataa aatgattata
     2641 aataaaagat aaaaatattg taaaaaagag ggtgcctctg gtgaattaaa ttcacacaga
     2701 gacacctttt taattaattt tttaaattag tatattattg tttaattaat agtatagtac
     2761 tatttaatta gtctaaattt ctaaggtctt ccataagctt agttttgtca gccgtttttt
     2821 catctttcat ttttataact ttagcaggag aaccagcaac aacagcacca gcttctacat
     2881 cagtagttac aacagcacca gctgcaacaa cagcaccttt accgattcta acaccttcaa
     2941 gaataactgc attagctcct attaaaacat catcttcaac tataacagga gtagcagaag
     3001 gtggctctaa aacaccagca acaacagcac cagcacctaa gtgaacattt ttaccaagag
     3061 ttcctctagc tccaataaca gcattcatat ctaccataga accttcacca ataacagctc
     3121 ctatatttat aacagctccc atcattacaa cagcattttt ttctatagta accatgtctc
     3181 ttattattgc acctggctca attcttgcat gttcacttaa agtatttttt agaggaatag
     3241 cagaatttct tctatcatat tctacatgag tatctgttat atcatttcca tgtttatcta
     3301 atacttccat tatatcatca tagtcaccta caaatatata agaaccatta tttccaaata
     3361 ccttaaattt atcagttgat tggaagtgta aagagttagt atttacatat accttaacag
     3421 gtgttttctt tttaacttct tttatatacc ttgctatttc ataagcatcg tttaaattta
     3481 ccatttgtta attctccttg tatttcatta tatttctttt attatatact tatttgtc
//
LOCUS       DAENCV010000232         3537 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAENCV010000232 DAENCV010000000
VERSION     DAENCV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.235"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            102..731
                     /locus_tag="KQD82_003761"
     CDS             102..731
                     /locus_tag="KQD82_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382196.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            732..1514
                     /locus_tag="KQD82_003762"
     CDS             732..1514
                     /locus_tag="KQD82_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382197.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1507..2385
                     /locus_tag="KQD82_003763"
     CDS             1507..2385
                     /locus_tag="KQD82_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG4382198.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2418..3377
                     /locus_tag="KQD82_003764"
     CDS             2418..3377
                     /locus_tag="KQD82_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG4382199.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLIANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
BASE COUNT         1409 a          348 c          644 g         1136 t
ORIGIN      
        1 aattgtttta ttaagaaaaa attaatatat tgacaaaatt taagtgctca tttaaactat
       61 taattgataa taatattcaa tattaaataa taggaggcgt tatgaataaa tatcttctaa
      121 taaatccagt ggcagaaaga atgtatggag aaaagtttca ccttattaaa gcaaatttga
      181 taaaaaaggg gtacataata gcagaatgtg aaccacagtt agaatatgta aaaaagcagt
      241 acaaagagta tacaaaaaag actaaaaata caatgttgga ttgtagatgt ccagaaagta
      301 taagtctttt aaaaagaaat aatttgataa atgattttga agtacctatg atagaaccaa
      361 tattagtaag gacttgtagg gttttatacg ataaatatat aaaaagtaaa gatgatatgt
      421 tggtagtaac ctgtccatgt acacaattaa gagactttgc aagtgaaaag tttaaggata
      481 agggtaatgc tatgttttat acatggaaag agtttgctga acaagaaggt gtaaaatcac
      541 ttggaaaaat agatgaatca ccaattccat taggatattt caaagatact tttgaaaaag
      601 ttcttgaagt atcaagtgaa gacgaaatat taagtgaaat acaaaaaata agaaatggct
      661 ttgaagataa gtatgacata gtagaaatgt tttattgtaa agatggttgt aataatggag
      721 atggtttgta aatggtcaat aaatttaata ataagttttt aaggctagta aagagtgttc
      781 tgatattttt tataattctt ttattatgga aaattacaaa ttatcttggt atttggagtg
      841 attatatatt accatcaccc gaaaaggtat atagtacatt tttaaatatg ataagtgatg
      901 gttcaatatt tattaatgta tatgcaagta tgaaaagagt tttgataggt tttgcaataa
      961 gtactgctat aggtgtgcct ttagggatat tctttggaat ttatagtgga gtatatgaat
     1021 actttaaatc tctaataaat tttttaagga atacacctcc ccttgcttta ataccaatgc
     1081 ttatattatg gtttggtata ggagaggaat caaaaattat aattatagtt ttagcatcct
     1141 ttttcccaat ctttacaagc acattgaaag ggattaaaaa ttgtgactca aaactgattg
     1201 aagtgggtag agtatttgag ttttcaaagc ttcagataat atttaaaata ataataccaa
     1261 atgccattct tgatattgct gttgggttaa agttagctct tggatatagc tttagagcaa
     1321 ttattggggc agaacttgta gcagcttcat ctggtcttgg atacttgata tctgatggaa
     1381 aagaaatgtc tagaacagat gttgttattg taggtataat cgtaattgga cttttaggaa
     1441 taattacaga ttacatattt tcgattattg tgaagaaggt aagtaaggga aagatggtgg
     1501 aagcatatga ataatttagt atgtgactct ggatatataa ttagaaattt atataaaagt
     1561 tataaagttg acaatgaaga acatgtagtt cttagtgata tctctttaaa catagataga
     1621 aaccatataa cagtaatttt aggtgaaagt ggttgtggaa agactacctt gcttagagca
     1681 attgcatcac ttgaaggtat aaatagtggt gaaattaggt ttattaatga taataaagag
     1741 tatagaccta atgttggttt tgtttttcag gagagcagat taatgccttg gttaaacgta
     1801 agtgaaaata tagcttttca taatcaaaga aaaaatacta ttttaaacaa ggtgcttaga
     1861 aaatttaaac acaataaaag aattagcagt aaaattataa atgataattt tagaagctta
     1921 gaaacaaatg ataatttgaa caaatcaaat aaatataata ggaaaagtaa tattgatgta
     1981 gaaaaatatc ttaagatgat gaacttgcag aaatttaaaa actcttatcc aaatgaattg
     2041 tcaggaggta tggcacagag agtaagcata gctagagctc tttctttcaa tccagatatg
     2101 ttgcttatgg acgagccttt ttcggcatta gattatttta caaggattga tatgcaaaat
     2161 gaagttataa gaatacataa gtcaactaat aaaggcgtga tatttgtaac tcatgatatt
     2221 gatgaagcgt taaagatagg caagaaaata gttgttttca cagacgaaag aaaaataaaa
     2281 gaatttaata tagaagatgg ttataataga gatttaactt cagattacta tattaaatta
     2341 aaaaaagata ttttaatgac aatgaaatca aataaaggca tttaaagaaa ttaataatat
     2401 tttaaggagt aaagtaaatg aaattaaaaa agctattgat aggtataacg atattaacac
     2461 ttgcaacagc atctttagct ggatgtgcta aaaaaaataa tggagaaaaa ctttcagaaa
     2521 taaatttaac ttatgtaaaa tcaccattaa atgttccttc tataattcag aaacaggatg
     2581 atttatttgg aaaggaattt aaaaaggata atataaaagt taacttccac gaaattacta
     2641 caggtccaga gcaaacacaa gctttagctg ctggagaaat agacttctta catgcactag
     2701 gtggtacatc agctttaata gctgcatcta atggagtaga gttaaagata ttaaacactt
     2761 atagcaggtc accaaaagga tttatgattt tgactaataa taattcaata aaatcagcag
     2821 cagatttagt aggtaagaag gtggcaggcc caaaagggac tatattacat caggtattga
     2881 tttcagcact tgataaagaa ggattaagta tggatgatgt ggaatttgtt aatatgggta
     2941 ttccagaggc atcagcagct ctttctgatg gcagcgtaga tgctgcattg attgctggtc
     3001 cagcagctct taaagctatg aagtcaggta gtaaacttat agcaaatggt gaaggtttag
     3061 tagatggtat aatagtaact gctgttagca cagactttgc agagaaacat ccagaaatag
     3121 ttgaaagatt tatgaaggtt gaaaaggaaa ctttagaata tgtaaataat aattttgatg
     3181 aagctatgga aaaagtagct aaagaagttg atttaagtct tgaagaaact aaagaattat
     3241 atgcttggta tgattttagt ttagatataa cagataaaga catatcttcg cttgaagata
     3301 cacaagactt cttaataaaa aataagttac aagagaaaaa agtgaacata aaagaattaa
     3361 tctataatgc taagtagaaa ctatgataaa ataatactaa gtaagaaata taataaaagt
     3421 ttaagttacc ttattaaggt aactttttct attttgaatt agtaatttca atttatttta
     3481 gaatgattag ttgataataa tgattaatta atcataaaaa gatacttttt tatagag
//
LOCUS       DAENCV010000233         3452 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAENCV010000233 DAENCV010000000
VERSION     DAENCV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.236"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     misc_binding    312..522
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            748..1563
                     /locus_tag="KQD82_003765"
     CDS             748..1563
                     /locus_tag="KQD82_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG4382200.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1591..2268
                     /locus_tag="KQD82_003766"
     CDS             1591..2268
                     /locus_tag="KQD82_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG4382201.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2327..3049
                     /locus_tag="KQD82_003767"
     CDS             2327..3049
                     /locus_tag="KQD82_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG4382202.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1392 a          340 c          631 g         1089 t
ORIGIN      
        1 gtgtattatt taacttatta gaaaaagaaa taagggttta atatattatt tatagttgtt
       61 tagaaaaagc aacaatgtgg aaaatattat ttagtattat taaagaaaag caacaatgtg
      121 aaagatatta tttagtatta tttaagaaat agctaaaaat atgaaatata ctatttataa
      181 ttacacaaat aatatcaata aatataaaca gtagtattta gataatttac aagtaaaaat
      241 tattaaaaaa ataaaaaact tattgacaaa aatattataa ggaagtacaa ttgtattaac
      301 aatttaagga aacaaatcca atgatagaga aagtagcata tatggattta cagcgagtta
      361 ggggttggtg taagcctagc aatgaagtct atgtgaagag agctctggag gagattatca
      421 gaaactttag tatgataatt ggtttacccg ccttaaggga ttgagaggat aagtacttac
      481 ttatcaaaaa gagtggtaac gcggatataa ttcgtctctt agctgtaaag ctaagggact
      541 tttttgattt taaaataaaa atatccatac agcttaaaag ctaacagtca caaattttga
      601 aagtaatata agtatttatc aataagagtg gtaacgcggt aaagtcgtct cttagtcatt
      661 aaagactgag agactttttt tgtaactcga aacatttaag tattaaaatc tgagggaaat
      721 tataattaaa agagaggggt agttaaaatg aaagtattta aaaaattatt aagtttagga
      781 ttagtattag gattgacatt atcgctagta ggatgtagtg gtggaggaga aaaaacaaag
      841 ttagaacaaa taaaagacaa tggaaaatta gttgttggta ctagtgcaga atttcctcca
      901 tttgaatttc ataaggtagt agatggaaaa gattcaataa aaggattcga tataatgttg
      961 gctgaagaat ttgcaaaaga gcttggtgta aaggttgaaa ttaaggatat gtcatttgat
     1021 ggattaatag gtgctttaaa tgcagaccaa gttgatattg ttcttgcagg tatgtctcca
     1081 acaccagaga gagaaaagag tgttgatttt tcagaattat attatttaag tagaaatgca
     1141 gttatagtta aagatgcaga tatagacaaa gtaaaaactg aagatgattt aaagaaactt
     1201 agagttggag ttcaagcagg aagtatccaa gaagaatatg tagttaacac tttaaaaatg
     1261 acaactacaa aatctttgaa agcaatacct gatttaatta cagaattaaa aaatggaaac
     1321 atagatgcag ttgttactaa tgaagcagtt tctttaataa atgttaaaaa atatgatgga
     1381 ataaaaatgg caaatactga agttgggaaa gatgtaacag aaggtatggc agcagcaatc
     1441 aaaaaatctg acaataacaa agattttata gaattattaa acaaaaagat aaaagaatta
     1501 caagatggca agaagataga agaattttta aatgaagcat ctactgaagc tgcttcaaat
     1561 tagtaagaaa gttatgtggg agatgaaatt atgagtttag attttagttt tttaagtaga
     1621 tttgggactt cttttttgga aggaacaggt gtgacagtat caatttctct tgtggcatta
     1681 tgctttggat ttataatagg tataattatc tgtatggcaa aaatatcaaa gagcaaagta
     1741 ttaagagcaa tatcatcaat ctatattgag gttttaagag ggacaccatt attagtacag
     1801 atatatatag tatggtttgg tttacctcaa ttaggaataa gattccctat gttgtttggt
     1861 ataccaagtg aatttatagc tagtgcattt gcattatcag taaactctgg tgcatacgtg
     1921 gcagagatac ttagatcagg tatacaatca gttgataatg gacaaatgga agcaagtaga
     1981 tcattaggat taaattattg gtcaactatg agatatataa taatacctca agctataaaa
     2041 aatatattac cttcattagc aaatgagttt attacacttg taaaagaatc ttcaataata
     2101 tctgtaatag gagttgttga gataatgcgt actgctgata tagtaaaaaa tgcagcattt
     2161 agagcattag agccattaat agtagcagca gcaatttact ttgttataac atttactcta
     2221 tcaagattag ttggattatt agaaaagaaa ctatctgtgt ctaactagat gagagcatgt
     2281 ttagcattta gggaaaacat agaatagtaa aaaggaaagt gataatatga ttacaataaa
     2341 aaatttaagt aaatcttttg gagatttaaa tgtattaaaa aatatagact tagaaatcgc
     2401 taaaggcgaa ataatggtta tagtaggtcc aagtggttct ggtaaaagta cacttcttag
     2461 atgtatgaat ttactagaaa tacctacagg tggagaaata atatttgaag gaaaaaattt
     2521 ggtcgataaa aaaactaata ttgatgaggt tagacaaaat ataggtatgg tgtttcagaa
     2581 ctttaattta ttcccacata agactatttt agataatata acattagcac caattaaatt
     2641 aaaaaagatg actaaggaag aagcagaaaa aaaagcagaa attttgttga gtagagttgg
     2701 tcttttagat aaaaaagatt catatccatc acaattatct ggaggacaaa agcaaagaat
     2761 agcaatagct agagcacttg ctatggaacc agatatgatg ttgtttgatg agccaacatc
     2821 tgctcttgac ccagaaatgg taaacgaagt tttggatgtt ataaaagaac ttgctaaaga
     2881 aggtatgaca atggcaattg taactcatga gatgggattt gctaaggaag ttgcagatag
     2941 agttattttt atagatggag gaagtatact tgaagataat actccagaag aagttttcgg
     3001 aaatccaaaa catgaaagaa caaaagcatt tttagcaaaa gtgctataat atataaaaag
     3061 gatgttattg atattagaaa ttcaatacat ccttttttag tgatggttaa tattaatttt
     3121 aaaattaaag ttatattaat attaacttta tttttacgat tttttgcaga aaaaaaaaga
     3181 gctcggggta agctcttttt ttccaccgaa ttaatttccg ttactaatcc gatttttaat
     3241 aaatataaaa tatatcctta tgaaagggta ttgggaatag atatattaac taactagggt
     3301 taccaggggg ataaccttaa tttattataa caaaatgcga cacaaaaatc aatatttttt
     3361 ttgaaataaa ttataaaagt tatttttata acaagtaagc ttataaattt aacttttata
     3421 gtaaaaataa gtatatgtat aatataatat at
//
LOCUS       DAENCV010000234         3438 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAENCV010000234 DAENCV010000000
VERSION     DAENCV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.237"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     repeat_region   104..1514
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=104..132
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            2161..3432
                     /locus_tag="KQD82_003768"
     CDS             2161..3432
                     /locus_tag="KQD82_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG4382203.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1345 a          478 c          423 g         1192 t
ORIGIN      
        1 tgttatctgg taattattta ctacaaatgc taaaaaataa ttatatttta aaggagaaaa
       61 tgctgtataa attattgggg tattttacct atacttgttg caaatttaca taccacttag
      121 ttaatataaa acaaatttca aagagattgg acaacgaata aaaattgcaa tttatatacc
      181 atatagttaa tataaagcat atttgtgtcg atatatagtt ctaactaagt ataagattta
      241 catacctcat agttaatata aaacacatat tttacatgtt tgaagatttt atcagaaacc
      301 ctatttacat accgcatagt taatataaaa ctattaatag tgctatgttg tttatttgca
      361 ttaacaagat ttacatacca cttagttaat ataaaacttg taatttagat aaatgttctg
      421 ttggagattt aaccaattta cataccactt agttaatata aaactgcaac agttcagcaa
      481 ggagctacaa atatgtataa atttacatac cacttagtta atataaaaca atatactata
      541 tttatcaact gatgaagtgt tgcaatattt acataccact tagttaatat aaaacttaac
      601 gaaattaaaa ttgaatctaa taattagcta taatttacat accacttagt taatataaaa
      661 cggcttgcaa accattcgac aatgctattt attatttcat ttacatacca cttagttaat
      721 ataaaactga aacttttaac actctacgcc atctctaaat attatttaca taccacttag
      781 ttaatataaa acagttgaat atcattttaa attaaaactt acatatttat ttacatacca
      841 cttagttaat ataaaactta taataagtat aaccaactat gcaatttgaa aaagaattta
      901 cataccactt agttaatata aaacttgtag acttcaaccc tacaatgcta gtaggttcag
      961 tatttacata ccacttagtt aatataaaac ctgtacctat tttaacagga aatctattgc
     1021 aatctttatt tacataccac ttagttaata taaaactagt ctcaactttc aaataatctt
     1081 ctttttctat cagatttaca taccacttag ttaatataaa acatcctagt cttgatgctg
     1141 gagataatat tgtaattcat ttacatacca cttagttaat ataaaacctc tttctggagt
     1201 tgcttacgat ttaccttata ctccatttac ataccactta gttaatataa aaccaattaa
     1261 tcccgacgtt cctatagttg gcattgattt atttacatac cacttagtta atataaaacg
     1321 accaagtacg tgttttaaag aaaaaacaag aagttattta cataccactt agttaatata
     1381 aaacacaata aaagtcagct cgaaatgttc atggaaaaca atttacatac cacttagtta
     1441 atataaaacg ctataataaa aaacagatgg ggtggagctt ctttgattta cataccactt
     1501 agttaatata aaaccccaaa ataaactaag tattttcagt acttacacac atacaactct
     1561 ctcaaatttg cagtgaacca tgagtagtgc aattgataac atttatcaca caccctcaat
     1621 cccttacatt gcaactgtta aaccctactt tatcaaaaat atcgctcact gcaaaattta
     1681 tatattttta ttatatcata aaattcaaac tataaaatta aaatggaata atttatcaat
     1741 ttaagaacac ctacatattt gtaaatactt actatattat ctattctaat aattcttctt
     1801 ctagctggaa ctaatataaa aatcatctct gaaagattag gacatactaa tataaaaata
     1861 actatgaata gatattttct tgttttaaac gatatggata aaaaagcttc tgaaaatata
     1921 atcaaagttc tctttaaata acattccatt ttatgtaagt aggcattata tttttcttaa
     1981 atgtcactta aatgtcagtt atatataata atagtaaaca actgtattgc actaatattt
     2041 gctattatct aatagtattt atagtttaga tttgatataa tacatgtggt aaaattttga
     2101 tttgtaaaac aaaattttac ctacatatat taaaacaaat ttaaaactaa ggagacctat
     2161 atgttaaaag tattaaaagg aaatataatt tttacaaaaa cttcagaaac tttcacagta
     2221 tttgaaaata gttatattat actggaaaaa ggtaaagtaa agggaatatt taaagagata
     2281 cctaaggaat acaaaaacct aaaagtagta gattacactg ataagcttat aatacctggt
     2341 atgaatgacc tccatgccca tgcctctcaa tttaaaaatc ttggaatggc tatggataaa
     2401 gagcttttgc catggcttga aacttataca tttccagaag aatcaaatta taaagatata
     2461 gaatatgcaa caaaaatgta taaaaaattt gtaaaagaac tttggaaatc tggaactaca
     2521 agaatagcag tgtttgcaac agtacataaa gaagcctcta tgaaacttat ggatatattc
     2581 aaagaagttg gtcttggagc atacgttggt aaagtaaata tgaatatgaa ttgccctgat
     2641 actttattag aaaacactca ggattctatt actgatacag aagaaattat aaataagtat
     2701 agtgatacag attctattgt aaaaccaata ataacaccta gatttatacc aagctgtaca
     2761 agtgaacttt taaaaggttt aggagattta tgtttaaaat ataatattcc tattcaatca
     2821 cacttatctg aaaattatga tgagattgaa tgggttagaa gtctagaacc tgaatctaaa
     2881 ttctatggag atgcatataa tagatataat ttatttggtc aaactcctac tttaatggct
     2941 cattgtgttc attgttctca ggatgaaata gatttaatga gagaaaataa tgttatggca
     3001 gttcactgtc cagcttctaa ctttaatgtt ggtagtggtg ctatgcctat cagaaaatta
     3061 atcgataata atattagatt agcacttgga tcagatataa gcggtggaca tacattgtca
     3121 atatttaaag caatggtatc tgcaatacaa ttatctaaat tatattgggt taattctggt
     3181 aagaagtata atttcttatc tttatctgag gctttttata ttgcaaccaa aagtggtgga
     3241 agtttctttg gaaaagttgg tagctttgaa gaaggttatg attttgatgc attgattatt
     3301 gatgattcaa atttaaatca tgacaattat tctatacttg aaagacttga aagatttatc
     3361 tatgttggtg atgatagaaa tatcattcat agatatgtat gtggaaaact aattgaagaa
     3421 cctaatatat aattataa
//
LOCUS       DAENCV010000235         3438 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.238, whole genome shotgun sequence.
ACCESSION   DAENCV010000235 DAENCV010000000
VERSION     DAENCV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.238"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            55..1065
                     /locus_tag="KQD82_003769"
     CDS             55..1065
                     /locus_tag="KQD82_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG4382204.1"
                     /translation="MVYFVSIELKDRSLVNNQYKGKIKSANLSVDYEINQVMKDIDKL
                     GLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKL
                     YETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEII
                     DFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKP
                     VNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNH
                     TPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSW
                     YE"
     gene            1119..2243
                     /gene="wecB"
                     /locus_tag="KQD82_003770"
     CDS             1119..2243
                     /gene="wecB"
                     /locus_tag="KQD82_003770"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG4382205.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLV
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2321..3295
                     /locus_tag="KQD82_003771"
     CDS             2321..3295
                     /locus_tag="KQD82_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG4382206.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1434 a          331 c          603 g         1070 t
ORIGIN      
        1 tttaaattaa aaagaagtat tttaatctca ttaataatct taatttttat atttatagtt
       61 tactttgtaa gtattgagtt aaaagatagg agtctagtaa ataatcaata taaaggtaag
      121 attaaatcgg ccaatttatc tgtagattat gaaataaatc aagttatgaa ggatatagat
      181 aaattgggat tgaatacagt aaatgtacct ataattataa atgtagagtc cattaattct
      241 gatattatgt caatagataa taatagtaaa gaaaaggcaa ttaagttaat aaaaaaatta
      301 aataaaaaag gtatttcaac aatattagag gcatatccat ggattgaaaa tggaaaattg
      361 tatgagactg actggaaccc tataaataaa aaaagatttt tttatacttg gcaaaatgcc
      421 atattaaatg aacttataat tgatgtagca aatccactgg atgtaaatgt tttaaatatt
      481 ggttctaatt ttgtacattt ggaagaatat caacaaaatt ggggtgaaat aatagatttt
      541 gtacaatcca aatttgacgg tcttgtaacg tacagaacta attggtggta tacaaaaaaa
      601 gatgatttaa gtagtaagtt gttttatgaa caaaagttga acaataattt ttttgataag
      661 ttagatttta tatctatagc agcatatttt gagttaagta ataaacctgt taatacagtg
      721 gatgaattaa tttctgcctt acactcatct actgtaaaca atagaggaca aaatataaaa
      781 caagaaatat ataaccttta taaagctcat agaaaaccta tattttttgg agaattaggt
      841 ttttcaaata gagaaagtgc aagttctcag ccgtggaatc atactccttc aaaagctgta
      901 aatggcgaag aacaagcaag gtgttttgag gcatataaga aagtttttga aaatgaagac
      961 tggataaatg gtttttctgt gttttgtgta ggtaaaatag atgacgaaaa aaatttttac
     1021 ccatcaaaag agagtataaa agttattaag tcttggtatg aataattttg aaagtgtaga
     1081 atttatatag attaaataga tgattaggag agatataaat gaatgacatc aaggttatga
     1141 ctgtatttgg aactagacct gaagctatca aagtagcacc tttaataaag gaacttgaaa
     1201 aaagagagaa tataaaaagt attgtttgcg ttacagctca acatagagaa atgcttgacc
     1261 aagttataga aacctttaat ataaatgtag actatgattt ggatataatg gaaaaaggac
     1321 aaagcctgaa tgatataaca tgtaagattt taaataaact tccgttgatt ttaaacaagg
     1381 aaaatccaaa tataatattg gtacatggag atacaaccac aactctagct acaagtttga
     1441 ctgcatttta taataaaact cttgttggtc atatagaagc tgggcttaga acctatgata
     1501 aatattctcc ttttccagag gagttaaata gacaacttac aggtattatt gctgatatgc
     1561 actttgctcc tactaatttg gctagaaaaa acttggtttc tgaaggaaaa cctaataata
     1621 atatatttgt aactggtaat actgctattg atgctttaaa gatgacaata aaagaaaact
     1681 ataatcatcc tataattgat gagattggta acgataggat gattttgtta acctctcaca
     1741 gaagagaaaa tttgggtaaa cctatgaaaa atatattcag agctataaag cgaatagtag
     1801 atgactttga agatgtacag atagtatatc ccatacattt aaatcctaaa atacgaacta
     1861 tagctgatga gatatttgga aaatttcctg aaaaaattca tattatagag cctttagatg
     1921 tagctgattt tcataacttt ttaaataaat cttatatgat aatgactgat agtggaggaa
     1981 tacaggaaga agctccatca ttaggtaagc cagttcttgt gttgcgtgat aaaacagaaa
     2041 gaacagaagg aattgaagca aagacactta aattagtagg tactaatgaa gatagaatat
     2101 ataattcagt gagcgattta ttaataaata aagataacta tgttcagatg agtaaagcat
     2161 ctaatccata tggagatgga aatgcaagca aatatatagt ggatataata attaaaaaat
     2221 ttaattgcaa atatttaaat tagataaggc ctaaaacatt aactaattga tataagattt
     2281 atgataatat taaggaatac aatttttaag ggggagtaat atggctagaa aattaataaa
     2341 aaatttgggt aaaagtaaaa gtgtaaagag agtgaaactt ttatttaaga agatttttat
     2401 tactgtattc atagtagcaa gtatagttgc tatttttaat ataacgaaat attttgagga
     2461 attatacaaa gtaagggatt taaaaagtac taaaattgaa tactatatgg atgtggctga
     2521 tgaagcagga gatggaaagg tccaattaag ttggaaggcc ttacttgcta tagacatggt
     2581 aatacatgat gaagatttaa gtaatataaa aaagaaagac acattggata taggggaaaa
     2641 gtttatagta gaagataaaa atggtaaagg cgaaaaggtg tataaggtaa aaaagtttaa
     2701 taaggtatta agcgaattga aatttgactc ttctcaaaaa agtagagcaa gaaaatacat
     2761 gaaagattta gaatacacat accttggaaa taaacaatta gatagtagtg atgaaaaaat
     2821 taaatttata aagaagttag aagactcagc tataagagaa tatattgatt atggaatatt
     2881 gccctctata acaattggac aagctatatt agaatctggt tggggaaatt ctaaacttac
     2941 aaaacagagt aataatttat ttggtataaa agcagataaa gcatggaaag gaaaaagtgt
     3001 agaaatttca acttcagagc attataatga aaaaattgta gctagtttta ggtcgtataa
     3061 ttcattacaa gattctgtca aagatcacag tttattttta attaataata aaagatatag
     3121 aaaacatggg ttgtttgagg caaaagatta tattagtcaa gctcaagcat tagaaaatgc
     3181 aggatatagt acagcagaag ataaaaaagg aaatcgtata tatgcagagc tattaataga
     3241 tgttattagg agttataatt tgcaattgat agataataaa gttgagacaa aataattgtt
     3301 aactttatat gaggagataa caagataaga tttttttcta caaaaagaat agaaaaattg
     3361 taaaaaaaac taaaaaaatg tattgacttg ttatttaaaa ggtgatatta ttattactgt
     3421 actcgaaaga gaacaaaa
//
LOCUS       DAENCV010000236         3404 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAENCV010000236 DAENCV010000000
VERSION     DAENCV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.239"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(122..298)
                     /locus_tag="KQD82_003772"
     CDS             complement(122..298)
                     /locus_tag="KQD82_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382207.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(352..441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(621..821)
                     /locus_tag="KQD82_003773"
     CDS             complement(621..821)
                     /locus_tag="KQD82_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382208.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(823..1173)
                     /locus_tag="KQD82_003774"
     CDS             complement(823..1173)
                     /locus_tag="KQD82_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG4382209.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1335..1524
                     /locus_tag="KQD82_003775"
                     /pseudo
     CDS             1335..1524
                     /locus_tag="KQD82_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2001..2171)
                     /locus_tag="KQD82_003776"
     CDS             complement(2001..2171)
                     /locus_tag="KQD82_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382210.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2560..3048)
                     /locus_tag="KQD82_003777"
     CDS             complement(2560..3048)
                     /locus_tag="KQD82_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG4382211.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSITIPNDKNMIITFLSNKS
                     DN"
BASE COUNT         1332 a          446 c          376 g         1250 t
ORIGIN      
        1 aatttttagt taacattttt atttattctt aatatgaaaa taaatttaaa cattttatat
       61 taccaatata ttaatgtaaa tgtagataaa actaaaaagg gttttctaga aaaaaatctg
      121 tctaattata cttacttacc aataccatca cttgatgcaa gcaagattcc acaacttttt
      181 ctccgtactc taatataaaa tcatctaact gtgtatctat aaaatcacaa taatcatatg
      241 aactaccaat ataagcagtt aacatatgtg tacatttatc cattttatct ctaaacataa
      301 tgtatttatc cccctaaaaa actttcatga aaaacaaaaa gaaaatatct ttttggcaac
      361 tggctgacat aacttatttt ataagcgtta gtccctatag ctttgcgtca ctaaatttct
      421 ctagttttgc cgatttagtt ttattctaca gattaataat acaatagaat tagttattat
      481 tcaacatatc tagctgaaaa attaataaat ttatatatga acgttaaatt atctgaaaaa
      541 ataaaaagcc cctctgtcag gagtgactta aaaattgaat ttaagtttgt atttaaaaca
      601 catttttaat aatgcaaaaa ctaatcaaaa ataaacaatt catcaatagg tgtatctact
      661 atttttgata tatctattgc taatttcaaa gaaggattat actttcctgc ttctaaacgc
      721 ataatggttt ctcttctaac tcctactttt tctgctaatt gttcttgagt taattctttt
      781 aattgtctat atttttttag gttacaagtg aatttaggca tattattttt cctctaaaat
      841 ataaagtttt atagcataaa catttaaagt gattgcatat gtaattgata gtaaaacaca
      901 cattaattct atattcctag acactatagt aatacaccat aaaatgaata attctataat
      961 agcaagctgt cctgtaaatg ccatagctgc tttttcatct tgtacatacc tttcatctgc
     1021 tttatcacca tttatttttg ctataataaa gtttgagaaa aaagcaaaaa atccaatata
     1081 tgtaaggtca gcaacattgc cagatgaaaa atatctaaga cttaaaaatc caaggaaacc
     1141 acaaaaaccc ataaaaaatt ttttctgaat cattttttat cctccttagt aattttaaaa
     1201 gagatatatt tatatcttta tgatacaaat atatctcttt tatattcttt tgtcaataga
     1261 aaatcagtat tatacaatat ttgtactgca ccataattgt tagagcaaaa atctactgct
     1321 tgagatgttt atgtatgact aaatatgatt ttaatttcaa attaaaagta gctaaatttt
     1381 atttgaatag agaaggaaga gatattttaa tagtaaaaca atatggagtt tataaccatt
     1441 ctcaagttga aagatgggtt aatatattag gagagtgtgc actaaaaata aaaaaaattt
     1501 tctatactat atggttcaag ttagatgtta taaactatat gatgataact aaatatagtg
     1561 tacaataaat cgctaagtat tttggaataa acaatagcac agttatagca acatgaaaat
     1621 aaaaattttt acatagtggt gttgatgcct tttctaaaat gaaaggaaga ccataatgaa
     1681 taaaaacaaa aacactaaag aaaaaataaa ttaacaagag aacaagaatt agaatatgaa
     1741 aataaatttt taaaagatga gcttgagttt ttaaaaaaga tgtgtgtctc agggaagcca
     1801 cattaaaaaa cagtggtatc attattaatc aaaagaaagt tagatgtatt atcaaaaaat
     1861 tagaacttaa atgcatagca ttttttcata aatcacataa atataactcc tataaaggta
     1921 taattggaaa agtaaaaaat aataaaacaa atagaagatt taaaacatca atagcatatc
     1981 agaaaataac aaatgatatg ctagaattta taaaatatat ctaatgataa aaatggaaaa
     2041 tcaattataa aaaaggcata tctcgattca ttttttgtta tatagtcaaa taagaatact
     2101 atagatgaaa gatacgattt tgcatatgta ttaagtaagt attctgatgt ttttggttga
     2161 tacacatgca ataaagaagt tgtgtttgca gataaattag caaaactata gtataagttg
     2221 caacaataaa gaaaatgaaa attatcatac taaagcataa ggcatgttac aattttatta
     2281 gataggacat taacatacac taaagtactt tttattataa agagtactta tttttttgaa
     2341 attcattaat gtataaacta tcaagaatat tactcaacat aatttaaaaa caacttgaat
     2401 aggtattttt aaattatgtg atataataaa aaatagaagt gtatgccaat acacttctgt
     2461 agttataact aagcattcta ttcttgagga gtgctttttt catttttata accaactctt
     2521 tttactttaa caataattat cagacctata tatttatttt taattgtctg atttattaga
     2581 taaaaaagtt attatcatat ttttatcatt tggtattgtt attgaactac cataatcttc
     2641 ttctaattta tctagaccac ttttacttat ttctccatct ataattatgt ttaattctaa
     2701 gtattcttca ctaccattag atgacactaa agtgtacgaa ccattataag gcatgcctaa
     2761 attttctatt ttatgtgcca aagggtcttc atcaccaata ccagagttta ctgttatttt
     2821 tccatctttt ttagtccacc atattatgtt tccttcagta tattttgatt catctgcata
     2881 gtagctaaga gacgcacttt ttattgcact tatatcagct tcaagtttag ctatctttgc
     2941 tttttctata tttttaaata atgctggaag tgctactatt gccaatattc ctattatagc
     3001 aattactact aataattcca ctagagtaaa tcctttttta tttttcattt atttcccctc
     3061 catataaaat taataatata taaattttta ttttgttgat ttctactttc tatattttac
     3121 catatataga caaaaaaatc actttctatg acaaatatac tattatttta caatttatgg
     3181 aaatgattta aaatgacatt tcttgttaga aaagatataa gaaatggatt aaaataaaga
     3241 aaactacagt catatcaatg tcaataatat tatttaaaag cagatgaaaa taaaaagagg
     3301 actcagaaaa taatgtctat ttaattatac ttacttacca ataccataac ttgatataaa
     3361 caagattata ctacattttt ttatgctcta gtataaaatc atct
//
LOCUS       DAENCV010000237         3362 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.240, whole genome shotgun sequence.
ACCESSION   DAENCV010000237 DAENCV010000000
VERSION     DAENCV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.240"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            63..401
                     /locus_tag="KQD82_003778"
     CDS             63..401
                     /locus_tag="KQD82_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG4382212.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAKAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            868..1371
                     /locus_tag="KQD82_003779"
     CDS             868..1371
                     /locus_tag="KQD82_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG4382213.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            1418..2377
                     /gene="moaA"
                     /locus_tag="KQD82_003780"
     CDS             1418..2377
                     /gene="moaA"
                     /locus_tag="KQD82_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG4382214.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLLENKCSDTNRRMYEIGG"
     gene            2381..2851
                     /gene="moaC"
                     /locus_tag="KQD82_003781"
     CDS             2381..2851
                     /gene="moaC"
                     /locus_tag="KQD82_003781"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG4382215.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2887..3318
                     /locus_tag="KQD82_003782"
     CDS             2887..3318
                     /locus_tag="KQD82_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG4382216.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1401 a          300 c          653 g         1008 t
ORIGIN      
        1 taaaaaaatt gtgagttaca tgatataatg tattaaatag gatactaagg agttgatata
       61 ttatgttaat agtaactaca gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat
      121 tagtgagagg aagtacaata agagcaaaac atgttggaaa agatatagga gcaagtttta
      181 agaatcttgt tggaggagaa cttactggat ataatgaaat gctcactgaa gcaagacaaa
      241 ttgccatagg tagaatggtt gaagatgcaa aagctaaagg tgcaaacgca gtaatagcat
      301 ttagactgtc ttcagcttca gttatgcaag gagcagcaga aatgcttgct tatggaacag
      361 cagttgtttt agaagatgat aatagtattc ttgaaaaata aaatataaat agttaaaaag
      421 aggcatcttt taaaaatttt atggatgcct ttttttattt gaaaaaacta atttgagaag
      481 aaattttaac tattatgaca ataaaattag ggtgatatta tataaataag taaaattata
      541 ttatataatg tatggtatag actttaaaaa ttttatttgt agagaaaaat atggacaatg
      601 ttataataat tatagaaatt aaaagtactt acattcatta tgtatgtgac atgtataatt
      661 agttttatat ttaatacagt tttgaagtaa tatcttcatt ttgagaacgt tggaataata
      721 aaaaatgtat gtattatagg tgatataaat aaaaaataaa tataatatgt tatattgctg
      781 atagaaaaga gaataaaaga aaataggttt tattaaaaga gatttttatt tataggactt
      841 aatcgatgta tagaggggtg agaacatatg tttaatgttg caattataac acttagtgat
      901 aaaggatatg aaggaaaaag agaagatata acaggaaaaa agttaactga atttgttgaa
      961 aatacaggag cttataaagt tactgaatat gtacttataa aagatgataa ggaaatgcta
     1021 aaggaaaata ttataagact ttgtaatagt aataaaatag atttaattct tacaaatgga
     1081 ggtactggtt tttcaaaaag agatataacc ccagaagcaa ctaaggaagt gttggaaaaa
     1141 gaaataccag gtctgagcga atatatgaga atgaaatcca cagaaattac taaaaaggcc
     1201 atactaagta ggggtgtgag tggaataaga aataattcta taataataaa tttacctgga
     1261 agtcctaaag gtgcagttga aaatctaagt tttataattg atgttttaga ccatggcata
     1321 gaagttttaa gaggagaagc tacagaatgt gccactaaaa aaagtgaata gtatataatc
     1381 tagatagata aaaaatagac aaagaggtag ataaagtatg attgataagt atggaagaaa
     1441 aatagactac cttcgtattt cacttacaga taagtgtaat ttaaggtgtg tctattgtat
     1501 gccaccagat gtaaaatttg ataaaaatta tataaatgaa aatctaagct ttgaagacta
     1561 caagtttatt ataaaggcta tggctgaaca aggtataaca aaaattagat ttactggagg
     1621 agaacctctt ttatatccta aattaaatga gcttataaaa tttacaaaag aagaatgtgg
     1681 aataaataac ataggaatga ccactaatgc tattgggctt tctgatagaa tctttgagct
     1741 tgaaaaatgt ggcttaaata aagttaatat aagtttagat tctttgaagg aatataagta
     1801 taaatccgta acaagaggag gaaacttaaa agatgtctta aaatcgattg aatcttgttt
     1861 aaacttaggt ataaaagtaa agttaaattg cgttgctata agtgggttta atgatgatga
     1921 gcttaaagat tttatgtttt taacagttaa atcaccaatt gatgtaagat ttatagagct
     1981 tatgccatta ggtgaggcta atagagttta taagaagggt tattttaatg taaaagaaat
     2041 gattgaaaaa atatatggtt tatataaagt caaaaatgat gaaaaaagta cagcagaata
     2101 ttatatgttt aaaggagcta aaggaagaat aggaattata actccattaa gctgttcatt
     2161 ttgtaacact tgtaatcgta ttagacttac ttctagtggt gcaataaaat tatgtctaca
     2221 ttcaaaagaa gaaatagata taaaaccatt tttacataaa ccattgttat ttagagaatc
     2281 tatgaaagat ataataaaac aaaaaccaga aaaacatcat ttactagaaa ataaatgtag
     2341 tgatacaaat aggcgtatgt atgaaattgg aggataagta atgttaacac atataaatga
     2401 aaaagggtat gccaagatgg tggacataag tgaaaaagat gatactaaaa gaacggccgt
     2461 agctacagct acaattactc ttagtaagga agcattagaa aaagtaaaaa atggtcagat
     2521 aaaaaaagga gacgtattgt ctgtagctca agttgcaggt ataatgggag ctaaaaatac
     2581 atcttcaaac ataccaatgt gccatcagat taaattagtt ggatgtgatt tagaatttga
     2641 tgtagatgat tctatgtctg ctattaagat atacgcaacc tgtaaatctc ttgggaaaac
     2701 tggagtagaa atggaagctt taaatgcatg ttctttagca gcacttacaa tttatgatat
     2761 gtgtaaagca gttgataaaa aaatggttat aagtaatatc catcttgtag agaagactgg
     2821 tggaaaatca ggagatttta agttcgagta aattaataat atattgggtt aaaatttgga
     2881 ggaaatatgg cgaaggtagt gtcaataaat ataagtgaga aaaaaggtgt aattaaagtt
     2941 cctgtacaat gtgcagagct aaaaataaat catgggatag ttggagatgc acatgcagga
     3001 aactggcatc gtcagataag cttacttgga aaagaaagca tagataaaat gaaaatgaaa
     3061 ggctttgacc aattaaaata tggagatttt gctgaaaatg taactacaga agggatagaa
     3121 gttttttccc tgcctgttgg tacaaagtta agaatagggc aatgtgaagt tgaagtaact
     3181 caaataggga aaaaatgtca caatggatgt gaaattaaga aattgacagg tgactgtgtg
     3241 atgccacgag aaggaatttt tgtaaaggtt ctaaaagaag gaattataaa tgtgaatgat
     3301 tcaatagatg taatctaatg aagtgactat taaaatataa ttataagtaa atactattaa
     3361 ga
//
LOCUS       DAENCV010000238         3340 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.241, whole genome shotgun sequence.
ACCESSION   DAENCV010000238 DAENCV010000000
VERSION     DAENCV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.241"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     misc_binding    164..375
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            538..1827
                     /locus_tag="KQD82_003783"
     CDS             538..1827
                     /locus_tag="KQD82_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG4382217.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGALLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1881..2960
                     /locus_tag="KQD82_003784"
     CDS             1881..2960
                     /locus_tag="KQD82_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG4382218.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2981..>3340
                     /locus_tag="KQD82_003785"
     CDS             2981..>3340
                     /locus_tag="KQD82_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG4382219.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYE"
BASE COUNT         1210 a          359 c          663 g         1108 t
ORIGIN      
        1 aaatatattt tgatgaaatt taaaaaaata cattatataa atatagtaat agtatgaaaa
       61 tttacatata aaattaaaaa atgcaagtaa ttgacttttt aagttgacaa aatattgcat
      121 atatattatg atataaatca taaattaact acaaatcaat attatatatt cgtagaagaa
      181 gaaagtagca taaaggaatt ttacagagag ttagatttgg ctgagaacta atattggaat
      241 ttatgtgaaa aggactttgg agaatactat tgtaattatg ttagaatagt ggtttaattc
      301 gccttaagaa aatgagtgag taaaattaat ttttactaat aagagtggta acacggattt
      361 atattcgtct cttggatttt tccaagaggc gttttttatt ataaacttat ttcagaaaat
      421 ttaaaactat atattaaatg aaaattataa aaatatgata gttaataaat ttataaatta
      481 gaaaattgta aaatatgcta aggtcagtag ttttacgatt taaatggagg gatgaaaatg
      541 gaagaagaaa aaattaacta tgctctggaa cagattccag agaatcaaaa acgtggttgg
      601 gtagctatgt tttcagtttt agtagcaata ggtgtggatt tatcatctgt tattttaggc
      661 gctgagttgg cacagagtat gccaatgaaa caagcaatat tgtcagtaat tgttggttca
      721 tttttttctg caattttgta tactacatgc tctttggttg gttcatctac aagtttatct
      781 acatctatga taacaaagta tgtatttgga gaagctggag caaaaatatt ttctctagtt
      841 ataggggttt ctctattagg ttggtttgga gttcaagtag gtttctttgc tcaaaatgca
      901 cagataataa taaaagatat atttaattta gatgtaagta tgcagatttt aagcctaata
      961 ggtgctctct tgatgatgag tactgcaatt tatggatata aggcaatgga gaagttgagt
     1021 gtgtattcag taccattttt attagttctt atgatgctaa ctatattctt agcttttaga
     1081 gcaaatggaa tttcagttga tgataatatg aaatctacta tgacatttgc aggtggagtt
     1141 tctctgtcaa tgagtataat aatagttggt gcaattgtat ctcctgatat aagtcgttgg
     1201 gctaaaagta gaagagactg tgctctttca agctttttgg gaatacaatt tggaaatgct
     1261 tttatgataa tagtttcgat agtattagta aaatgtatgg gaacttcaga tattatgaga
     1321 atttttatta cattaggaat tgctatacca ggtataatag tacttacatt agcacaatgg
     1381 actacaaata caagtaatgt atattcagca tcattaagta tagctcttgt acttaagaag
     1441 gctcctgaga aagtattaac tatagtcctt ggtattatag caacattatt ggctgtattt
     1501 ggaatatatg aaggttttat aggattttta aatttacttg gaattgtaat tgcacctgtt
     1561 ggtggtgtat atacagcaga atattacata gtaaagcaag aacttaaagg ctttgataaa
     1621 ggagtattat ataaaccaat agttaaacgt tcaatagtgt cttggataat tggtatatta
     1681 ataacttacc tttcaacata tggatttata actcttacta ctatagcacc tttagatggt
     1741 tttatagcag ggtttgtagt acaatctatt attggaaaag tgctttgttc aacaaaaaat
     1801 aagcaagaaa tagacaaagc agtataaaga aatttgttta taaagtataa aataaattag
     1861 aaatatgagg agaaagtaac atgagatttt taacagaaga aagtgtagat aaaatagcag
     1921 ttggtgcagc agtgcttgga actggaggtg gaggtgaccc atatgtaggt aaattggttg
     1981 caaaacaagc cattaaaaag tatggtcctg taaaagtaat aagtttagat gaacttgatg
     2041 atgatgcttt ggtagttcca gtatctggta tgggttctcc tgtaattaca atagaaaaat
     2101 tactttcaga agttgagtta actacccctt tagaaataat ggagaaatta ttaaatagaa
     2161 aagtggatgt tataattcct atagaaatag gagggataaa ctcacttatg cctatagcag
     2221 ttgctgctaa aaaaggactt cctatcttag atgcagattc tatgggtaga gctttccctg
     2281 aagctcaaat ggtaactttt tatcttgaag gatatgaggc atctcctgcc gtaatgtctg
     2341 atgaaaaagg taattctgcc atcctttatc ctgtggatgg aatttggtca gaaagattag
     2401 ctagaacttt aactgtagaa atgggaggaa gttcttctat tagtgactat aatttaagtg
     2461 gagctcaagt taaaaaagct gctataggta atactcttac aatagctgaa actataggag
     2521 ggtttttact tgaaaataaa tctgattcaa aaggtgctgt tgagaatata ctaaaagaat
     2581 tgcgtggata caagttattt gaaggaaaag taattgatat taagagagag ttgaaaggtg
     2641 gatttactag aggacatgcc ttttttgctg ggattaatga gtatgatgga gagtattcta
     2701 tattattcca aaatgagaat ttaattgcca aaaaaggtga tacaccactt tgtattacac
     2761 cagatttaat agcagtatta gatttagaaa ctggatttcc aataactaca gagagaataa
     2821 aatatggctc tcgtgttatg gtagtagctt ttccttgtaa tgaaaagtgg agaacagaaa
     2881 aaggaattga gacagttgga cctggatact ttggatatga tgtggaatat aaaacagtag
     2941 aagaattaca aggaaaataa aagtttagga ggaaattttg atgtatcgtg taggaattga
     3001 cgttggagga acaaatacag atgcttgtat actggatggg gaattaaaag taattcattc
     3061 agttaaggtg gcaactacaa aagatgttga aactggtgtt tacaatgcct taaaaaaagt
     3121 tatagatgaa agtaagattg accattcatt aataaaatat gctatgcttg gtacaactca
     3181 ttgtacaaat gctattgtag aaagaaaaag acttaataat gtggctatga taagaatagg
     3241 aaagccagcc actactgcca ttcttccatt tatagattgg ccagaagact taagagaaaa
     3301 aatagaatta gattcaatat tggtatctgg tggatatgag
//
LOCUS       DAENCV010000239         3206 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.242, whole genome shotgun sequence.
ACCESSION   DAENCV010000239 DAENCV010000000
VERSION     DAENCV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.242"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            141..1646
                     /locus_tag="KQD82_003786"
     CDS             141..1646
                     /locus_tag="KQD82_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG4382220.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2036..3133
                     /gene="dinB"
                     /locus_tag="KQD82_003787"
     CDS             2036..3133
                     /gene="dinB"
                     /locus_tag="KQD82_003787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG4382221.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
BASE COUNT         1276 a          336 c          574 g         1020 t
ORIGIN      
        1 tcttgaaaaa aattaatctt aattgtataa tattactttg agtgaaaaag tattaacatt
       61 tttttataag atgttttact aaaaaaatat atataaatat actgttatca ataagaaatt
      121 taatttagtg aggtgttaag atgaaagtag gatttgacca caaaaaatat ttagaagagc
      181 agtctaaata tatcttagaa agagtaaata attttgacaa actttattta gagtttggag
      241 gaaaattgat gggagacctt catgcaaaaa gagtacttcc aggatttgat gaaaatgcaa
      301 agataaaagt attacaacat atgaaagaaa aagttgaagt tgtaatttgt gtctatgcag
      361 gagatataga aagaaacaaa ataagaggag actttggtat cacttatgat atggaagttt
      421 taagattaat agatgattta agaggttatg aactagatgt aaatagtgta gtaattacta
      481 gatttgaagg acaacctgct acaactgtat ttataaataa attagaacgt agaggaatta
      541 aagtttataa gcattatcca acaaagggat atccatcaga tgttgagact atagttagtg
      601 atgagggata tggagcaaat gaatatatag aaactacaaa gccaatagta gtagtcacag
      661 caccaggacc taatagtgga aaacttggta cttgtctaag tcagttgtat catgaaaata
      721 aaagaggtaa tgtagttgga tattctaagt ttgagacatt cccagtttgg aatgtacctg
      781 taaaacatcc tttgaatata gcttatgaag cagctacagt tgatttaaaa gatgtgaata
      841 tgatagattc attccacctt gaaacgtatg gtgaaatgtc agtaaactac aatagagatt
      901 tggaattatt tccagtgctt aaaaaaataa tagaaaagat aactggaaaa gaatcaattt
      961 tccaatctcc aactgatatg ggtgttaata gagttggctt tggaataata gatgatgaag
     1021 taataagaaa agcttctata gaagaaataa taagaagata ctttaaaact gcctgtgagt
     1081 ataaaaaggg acaagttgat aaaggtgctt atgatagaat gaaaatgatt atggaagagc
     1141 ttaacttaaa gccagaagat agaaatgttg tattacctgc tagaaactac agtaataaat
     1201 tgaaagaaag tgccgacaag aatgatactt gtcctgtagt tgcattagaa ttagaagatg
     1261 gtacaatatt aactggtaag ggttctgatt taatgaatgg aactgcagca gtagtattaa
     1321 atgccattaa acatttagcc aatatatctg atgatatgca cttaatatca cctgtaatat
     1381 tagaacctat cataaactta aaaacaaaga ctttagctag tagaaatgtt tcacttagtt
     1441 gccaagaggt gttaactgca cttagtattt gtgctgtaac taatccaact gcacaatttg
     1501 ctatggaaaa gttagcatta ttaaaagggt gtcaagctca ttctactact atattaaata
     1561 gagatgatga acaaacattt agaaagcttg gtatagatgt aacttgtgac ccagaatatc
     1621 cttcacaaaa tctttactat agttaaaatt atataaaaaa atatgggctg ttttaaagta
     1681 atagtttgag cagtccatta caattataat aaaatcagta ggctgtcttg tataagacag
     1741 tcttttgttt ttaaaacatc ccataaatta aatatatata ctgttatcca taaggaattt
     1801 taaatttagt tccttggaat acctattgta aagcttttat atagtttttg caagtgtttt
     1861 taaggatttt ttataaatta tattgaagtt aatggattat agatggtttt atttatggaa
     1921 aatatgaata tataaagaaa ataatgaaaa aataccaaaa agaaacataa gtttggtata
     1981 attaaaaagt aatataaata aaattaatct atttatactt taaaaggtga taataatgaa
     2041 acattataga aaaataattc atatagatat ggacgctttt tatgcatcta tagaacaaag
     2101 agataataaa aaattaaaag gaagacctgt tattgtcggt ggaaatccac aaagccgagg
     2161 tgtggttgca acgtgttctt atgaagctag aaaatttggt atccattcag ctatgccatc
     2221 agctgtagct tacaataggt gtccatatgc agtttttgta agacctcgat ttgatgttta
     2281 taagagtgtg tcagagcaaa taagagatat attttataga tacacggatt tagttgaacc
     2341 attatcttta gatgaagctt acttagatgt aacaaaaaat aaaaaaaata ttgattcatc
     2401 aattgagata gcaaagcaaa taaaaaaaga tatatttaga gaagttggtc ttacttcttc
     2461 agctggtgtg tcatacaata agtttcttgc taagattgca tcagacttaa gaaagccaaa
     2521 tgggttaact gtaataaccg aagaaaatgc ccaggacttt ttagataaat tacctgtgaa
     2581 taaatttttt ggtgttggaa aagttacttc aaatactctt aaaaatttag gtataaaaac
     2641 tggatatgat ttaaggtgtt taaatttatt tgaacttgaa aatatattca aaaaaagagg
     2701 ttatgaatta tataaatttg ctagaggaat agacgataga ccagtggaac caaatagggt
     2761 tagaaaatct gttggggctg agactacatt aagtcataat ttggatattg atgaagaaga
     2821 aacacgcaac attcttgatg aactttgtga agaagtatgt catagattaa aaaactctga
     2881 gaagtttgga aaaacattaa cattaaaaat taaatatgag gactttacaa agataactag
     2941 aagccttagt ttagaacact atattgacga atataatgat ataagaagtg gtgtagataa
     3001 tttgttgaga aatgttgagg ttaatggaaa acaaattagg ctattgggag ttacaatatc
     3061 aaacctaagt gataaaaaag aaacttataa agatattaca ttatttgaat acatggatag
     3121 tatccaaatg tgagttttgt ttaaatgaaa taacactagc tttaatacta tataattgag
     3181 gctagtgtta ttttatgaat attttt
//
LOCUS       DAENCV010000240         3161 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.243, whole genome shotgun sequence.
ACCESSION   DAENCV010000240 DAENCV010000000
VERSION     DAENCV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.243"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            139..>3161
                     /gene="cbpA"
                     /locus_tag="KQD82_003788"
     CDS             139..>3161
                     /gene="cbpA"
                     /locus_tag="KQD82_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG4382222.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDANAGNAGGDTNAGNAEGDANAGNAGGD
                     ANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAEDPTSVGDFKI
                     SGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKT
                     ASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRD
                     IEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNT
                     ESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAG
                     IGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTN
                     RGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLE
                     KKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDK
                     FTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRY
                     DGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEETMEPDINLG
                     SDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESK
                     AKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSE
                     SHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVD
                     GDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDK
                     NIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNG
                     GNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKP"
BASE COUNT         1197 a          371 c          716 g          877 t
ORIGIN      
        1 caaatataag ttttaagtat atattaaaaa ttattagagt gtatataagg atgtatacag
       61 tgtatattaa gaattattag agtgtatata aggatgtata cagtgtatat taaaaattat
      121 taaggggagg gggaatttat gcaacaaaaa tgtaaaaaga aagcactatc aatgatttta
      181 agtatctcaa tgggacttgg atcagtagca tcatttaatg cgtcaaatac tttcgcagat
      241 actatagaag aaagtactaa tgcaggaaat gcagaaggag atactaatgc aggaaatgca
      301 ggaggggatg ctaatgcagg aaatgcagaa ggggatgcta atgcaggaaa tgcaggaggg
      361 gatgctaatg caggaaatgc aggaggagat actaatgcag gaaatgcaga aggagatgct
      421 aatgcaggaa atgcaggagg agatgctaat gcaggaaatg cagaaggaga tgctaatgca
      481 ggaaatgcag aagaagattc taatgcagga aatgcagaag aagattctaa tgcaggaaat
      541 gcagaaggag atatgaatgc attagttgtg gaagaagcca ctgcagaaga tcctacatct
      601 gtaggagact ttaaaattag tggtggagaa ataaatgtcg attttaaatt tgcaagtggc
      661 gttttaacta ttcttagtgg aaaacaaatg acaattcaaa ataataatcc aaatactcca
      721 acaacacata ggattgttat acctaaagat gtaaaagctg aattaatatt tgatggagta
      781 aatattaaaa ctgcatctaa tagtccattt actttaacac cagataaaga tggtggtgca
      841 tatgcacatg ttatattaca agatggtagt gaaaacaagc tagagtcagg tgatgtatat
      901 tatccaggta tgagagcagg aaaaggtact actctaatta ttgatgattc tgttgtaaat
      961 agagatattg aaggcgagtt tatagttcct gagggtggaa aaattcctag agatgtaact
     1021 ctagaaaatg gagacaagtt aaaaaaagga gatcgcctta cactactaga tagtaataat
     1081 agaggaagtt taaaagttgt tggaaattat tattctgcag gtattggcgg tggaaatact
     1141 gaaagtggag gaaatattat tattaatggt ggtgctataa ctgctatagg tcaagaatcg
     1201 agtcgtaata caggatatgg agctggaata ggtggaggcg gtaatggtag tggtggagat
     1261 atcacaatca atggtggtac tataactgct aaaggtgcat accatggagc tggaataggt
     1321 ggaggatact atacaacaac aaagaatgca ttaagtccct cagtagtaga tcctagcaca
     1381 ggttctggtt atagtgggaa tattacaatt aatggtggac tttcctattc atatggaggt
     1441 gcacatggta gtgcatttgg ggatggatgt gttaatccag gaacaaatag aggatatcgt
     1501 atacttgtaa ctggaggaac gattttacct tatatgacag gagtgggaga ttggattttt
     1561 gacctaagtg ctacaacggg agatgttata gtaacaggtg gttcattaaa agcaacaaca
     1621 ttcaaaagtt taggtggagc agttgcatac ggtgatttag aaaagaaaac aaaagttttt
     1681 atgaataaaa tatctctaac gtatttaggg caagataaag ttagtactac actagttgat
     1741 gattttaaaa tgctaattga tggaaaaatt tatccttatg gagcacctag ttatacagat
     1801 gctcaaggga ttctttactt atggttctct gataaattta ctggatctga agtatctgtt
     1861 aatttagttg ttatagataa aattactgga gaagttctaa aaccagatga tttttatatt
     1921 aaagatatag gttcaggttc tcaattttta aagcaatatg aagaatttac tattgatgat
     1981 actccaccat atttgattat taaaagatat gatggattac catttgaaga agacgtagaa
     2041 aaatcatttt tagattggct attaaaagat ggtggtattc caataactac tcctaaaggt
     2101 gaaaaactca cagataaaaa atttatgact attagttctc aacgtttaaa tgaggaaacg
     2161 atggaaccag atataaattt aggaagtgat gatcttacaa aacaagttga tgctgggaaa
     2221 tatcaattaa gcattacatc aacacagttt tcgagtatat atccttttaa ggatgcttat
     2281 tgggggcata gagcatacta taaggcagaa attattcctg ctgatacaga aacttcatta
     2341 gttgtagaag agtctaaagc taaaactgat gtttttaaac caacagatac gtttacatta
     2401 aatgttacta tttcaccaga taagaaagaa ggaccagatt gtgcctctcc aagaggttac
     2461 gtacaattct atattaatgg aaaggaatat aaagatccag tagaattaaa aactcataat
     2521 aagggtagtg agagtcataa ttatagtact gcatctattg aatggagacc tttagatagc
     2581 aatcatcata tttttactac tgaacaaact attactgtaa aatatattgg agatgatata
     2641 aattacattg aaggtagtga ggatgaaaag aagttaaagt tagatatgac aaatgtagat
     2701 gtagatgggg atggaatacc ggatataaat atagacactg atggtgatgg aaggccagat
     2761 atcaatatag atacgagtgg tgattggaag ccagatatta acatagatac tgataataca
     2821 ggtgaatgga agcctagtac agaaggtgga aatggagatg gtatttggaa acctgataaa
     2881 aatatagata ccaatggaga tggaaaccct gatacagatt ataatcgtcc agcaatagat
     2941 acagataatg atggagtaga tgattattgg aagcctgata aaaatgtaga tactggctca
     3001 ggaggttatg atacaggtaa tccaaatcta aataagccag atccagataa tggaggaaat
     3061 agcaagccgg atccagataa tggaggaaat agcaaaccag attcaggtaa tggaggaaat
     3121 agcaaaccag attcaggtaa tggaggaaat agcaaaccag a
//
LOCUS       DAENCV010000241         3068 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.244, whole genome shotgun sequence.
ACCESSION   DAENCV010000241 DAENCV010000000
VERSION     DAENCV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.244"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            72..1514
                     /locus_tag="KQD82_003789"
     CDS             72..1514
                     /locus_tag="KQD82_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG4382223.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1556..1909
                     /locus_tag="KQD82_003790"
     CDS             1556..1909
                     /locus_tag="KQD82_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382224.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2100..2921
                     /locus_tag="KQD82_003791"
     CDS             2100..2921
                     /locus_tag="KQD82_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG4382225.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1180 a          315 c          550 g         1023 t
ORIGIN      
        1 actatagcgt agatgtaata ctatgttata gtcatttttg aattaaactt gatatatcaa
       61 gggggaagaa tatgagaaca attagaagag ttttagcatt aggtttaaca ttagctatat
      121 ttctaataaa tgtacctaat gtagatgcat taacatcaga tacaattaaa ggtaacaata
      181 tatatgagac ggctggatta atagcagata aaaagagtta tgatacagca attatggtaa
      241 atatggataa ttctatagca gatggtcttt cagcaagtgg tcttgcaggt gctgttgatg
      301 ctccaatttt gcttgcacaa aaaaataaga taccaaatga aacaaaacaa agattaaaaa
      361 atgttaaaaa gatatacata ataggtaaag aattatcaat aagtaagtca gtagaaactg
      421 aactaaaaaa cacaggggca caggtaacta gattgggtgg agatgacaga ataaagacta
      481 gttatagtgt tgctaaagag gtaaatggta tcaaaaaagt tgatgaagtg atattaacta
      541 atgcatataa aggagaagca gatacaataa gtgctgcacc tgtatcagta agagatatag
      601 ctcctatagt acttacagat ggaaagagtg tgcctttttc aacaagtaat gtaaaaacat
      661 atgcagtagg tgggagtata tcaatgagca ctagtttagt taataaaact aatgctaaaa
      721 gacttggtgg ttcagataga tatgatacaa ataagaaagt tataaaagag ttttatccag
      781 atgcatcaga attttatttg agtgatggat atgatttagt aaatgcactt acaggttcta
      841 caattgctaa ggaaaatcca attgtattgg tgtcagaaag tagtgataag tctatattag
      901 caggagcaga taagattact agattaggtt caataagtga cagtgtgtat aataagtgtg
      961 tttctgctgc acaaaataat ggcgattcgt ctacaaaagg tgagtcacct atgaaaaatg
     1021 agacaagcat attaggtcag ccaacagcta gtttagaagc gtgcttaaaa tgggcaaaat
     1081 ctaaaaaagc taatgattta tttatagagt taataccaat attatatgat actgctgttc
     1141 aggaaggtgt taatcctgtt ttggcagtag ctcaatctgc aaaagaaact ggtttttgta
     1201 attttggtgg agtattagat gcatcattta agaatccttg tggacttaaa acttctgtag
     1261 gtggttctga tactgataag aatgcccatt caagatttga tacttgggaa gaaggaatat
     1321 tagctcaaat tcaacattta tgcttatatg caggacaaga tggttaccca ctttcaaatc
     1381 cagtagaccc tagacatgaa aaatctttat ttggtaaggc taaaacagtt gaaagtttat
     1441 ctaataattg ggctggaggt caatatggac aagatttagt aagaatgatg ggagaaattg
     1501 aggcaacaaa ataattgaag attatcaaat atccaataaa taaggattga ttattatgaa
     1561 aaagagtaat atttttagta tcatatttcc tacagtcata atggtgctaa tgtttttaac
     1621 ttttgctaca gatatattta atattccaga tattcaatct aaaagtattt ttgttgtggg
     1681 aatggttttg atttttccaa tttctttttt gatacaaggg ataatttgtg tattaaataa
     1741 gactaattgg attttatctc ttattgtgtc tttagtaact tatatagtat taatgtatat
     1801 atttttaaat gattctgcat atatatatgt tttactttat acagcttttt atatgattgg
     1861 acatataaca gctaaactct attacaaaat taagactttt aaaaattaaa ttgaagtata
     1921 taaaagtaac attaagagaa gtatccttaa tgattttcta aaatcagaaa taggtgcttc
     1981 tcttttttgt aaaaataaaa gtatgtaaat aaaatttaac cattataatt ttcttttaac
     2041 aagtgtatac aataaacttg ttgtataatt aagatagata aaataaaggg agggataata
     2101 tgaatttaat agataatcat attcatacta atttttcaag tgatggaaag gattccatgg
     2161 aagatacaat aaaaaaagct ataagtattg gtgttagata tttgactttt acagaccatt
     2221 tagaacatga tgaagataga ggattttgca taaattataa tgactatgtt ccagtattta
     2281 ataagtttaa agaaaaatat aaaaaggaca tagagttatt actaggtgtt gaagtgggtt
     2341 acagaaaaca tctaaaaaat gaaatagaag aaataataaa ttctaatcct tttgattttg
     2401 tattgtgctc aactcataca attgacaatg tacctgttcc atcaaaggcg tattttaaag
     2461 gtcttagcaa agaagatgcg tattataaat attttaatag catacttgaa actaatcgtg
     2521 aatttggaga ttacaatata tatggtcatt tggattatat atctaggtat ggaatatatt
     2581 cagataatag agttatatac aatgacttta aagatgttat tgatgaggtc ttaaagtcaa
     2641 taatcaataa tgggagtggt atagagctaa atacttctgg ttatagatat ggactaaatg
     2701 ctatacatcc aaatgaagat attctaaaaa gatataaaga acttggagga tttatagtta
     2761 ctgtaggttc tgattctcat agggtagaag atatatgcaa agattttgat gtggcctatg
     2821 atatgcttaa gtatcttgac tttaaatatg tatctttatt taaagaaaga gaaacttatt
     2881 ttgtaaatat agaaaaagtt aaatctaaca atatagctta gagtgtgttt tgaagtaaat
     2941 taatggatag ttaaaaagtt aatagttata atattgtatc ctctagaaaa atactagagg
     3001 tttttatttt gctaaaataa ctaaatagaa ttaaatttaa atagtatagc ttattttaaa
     3061 ttgaatag
//
LOCUS       DAENCV010000242         3061 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.245, whole genome shotgun sequence.
ACCESSION   DAENCV010000242 DAENCV010000000
VERSION     DAENCV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.245"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            101..679
                     /locus_tag="KQD82_003792"
     CDS             101..679
                     /locus_tag="KQD82_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG4382226.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNKLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            970..2220
                     /locus_tag="KQD82_003793"
     CDS             970..2220
                     /locus_tag="KQD82_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4382227.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            2381..2950
                     /locus_tag="KQD82_003794"
     CDS             2381..2950
                     /locus_tag="KQD82_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG4382228.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVNELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         1142 a          401 c          454 g         1064 t
ORIGIN      
        1 atcacttgac aaatttataa gtagacaata ttatatatct atgactcaaa atattttcaa
       61 agtcatatat atcatatata gacagaaagg attgataaaa atggctaaaa gttttacaga
      121 aatagaaaaa gacaatataa gaaaaagact aatttcagaa tgtgaaatca actggtctaa
      181 atatggatac aaaaaaacta atattgatga actttgttca aaatcaggaa ttgcaaaggg
      241 ttctttctat acattttttg attcaaaaga aaagttattt tttgaagtat taaatggaat
      301 ccaaaacaaa cttatatcat cagtagatga aattttaagt aacatgcctc caaaagaagg
      361 ctttgcaaaa tccttaaaaa tgttatatcg attatatgat aaaaatccat ttttatattc
      421 accaaataat cctgatttca tcagcctaat aaataaacta cctcaagata tgtttttaca
      481 acttgagtcc aataacatac aaagttttta cgacttaatt gagagacaca atctcaagtt
      541 gaagatagaa aaagaaaaag tttttggtgt ttgtaatgct ctactatcaa gtctattggt
      601 aaaagaaaat attgaatata actatattga agtgtttgac tttatggtgg ataatcttgt
      661 agaacacata cttgaataac aatttaaaat aatttttcta agtatttttt tatactaaat
      721 aggattaata tgattgaatt aatgaaaaaa ataaacaata taatttataa aataaatgat
      781 aaactaaata tcacacaaaa cttatagacc ctagagcaaa cccattccac tctactctaa
      841 gccatctata atagtttgta tatttctaat aatattagat attaaagtaa gaaaaaaatt
      901 ccttataata attgctatta tatcaattat tataaatatt ttaataatct tagataggta
      961 ggtacatata tggtaaaatc ctcgaacact tttaataaaa acttcaactt gatgatatta
     1021 ggtcaaataa tctcattgtt tggtgcatca atactcaagt ttgctttgtc tttatacata
     1081 cttgatataa ctggtaaagc agaaatattt gctactatac tagcagtatc ttcaatccca
     1141 attatagtat tctcccctat tggtggagct attgcggata tattcaacag aagaaactta
     1201 atggttattt ttgatttttc aagtagtctt acagtactaa tcttagcagt attcttattc
     1261 aacaataatg gttcaatatt acttgtagga attattatga ccatattatc agtcataagc
     1321 acaatgtatc aaccaactgt tcagtcaagt acaccccttt tagttgataa tgagcattta
     1381 atgaatgcaa atggtattgt agctggagtt gcatctctta caaatattgc aggacctgta
     1441 ttaggaggtg tcttgtatgg tgtgattgga attaatgcaa ttatagttat aagttgtgta
     1501 agtttctttc tatctgcaat tatggagata tttatacaga ttccttttat caaacaagag
     1561 caaaacagca acattataaa aactatattt actgatataa aggatggtat tgttcatata
     1621 tgtaaagaaa atagatttat actaaaaata ctttttttag ctatagcaat aaacttattt
     1681 ttaagctcta tgttaatagt tggtatcccc tatatagtaa aaatcactat gggagctagt
     1741 aacttcttat atggtctagc agaaggagca atttctttta gttcactggt agctgctgtt
     1801 agtattggta ttttctcaaa acatctaaaa atcactaatt tgcacatttc ctttattata
     1861 tgtgcagtta tgttcatacc aatggcggtt tcagtatatc catttatatt aaacacagga
     1921 tttccagttc catttttaat ctttatagta tcatctatgc taatattttt tacaacgact
     1981 cttatctcaa tatatgttat aacagtcaca caattaaaaa ctccaaatga actcttagga
     2041 aaagtaatgt caattctttt tgcaggaaca gcaattgcaa tgccccttgg tcaggttatg
     2101 tatggtaaac tttttgattt attatcagat agagtgtaca tcattatact aggagttgga
     2161 tttattacat tacttatagc atttgtatca aagataacat tgaggaacga aaatctttag
     2221 atacaaagaa tttagatata aagatttaat tttcactaat ttctgaattt aaatgcaaaa
     2281 ttgtgtatca gtatgttgac gttttagaaa ttgattgata taatcaatgg aaaataaact
     2341 ttataaaaat tcaaaatatt cagctagagg agtgataaaa atgagttaca tgccaacttt
     2401 agaacaagca tgggaaatat taagaaaata caataaggaa gaatttcata taagacatgc
     2461 tcaaattgta tctggagtta tgagatacta tgcaaaggag tacgaccctg aaagagtaga
     2521 attttgggaa atagttggac ttttacatga tttagatttt gaaatgtatc ctgatgaaca
     2581 ttgtgttaaa caaagagaat taatgacaga gcttgattta gataaatcaa taatcgattc
     2641 tacaataagt catggatatg gattaactgg ttcagatgtt aaaccagagc aatttatgga
     2701 gaaagtatta tttgctgtga atgaacttac tggtttaatt ggtgctgctg caataatgcg
     2761 tccttcaaag agcgtttctg atttagaatt aaaatctgtt aagaagaaat ttaaagataa
     2821 atcatttgct gctggctgtt ctcgagacgt tataagaagt ggtgcagaaa tgcttggatg
     2881 ggaacttgat aagcttatcc aaacaactat agaagctatg agaagcttga ttccagagat
     2941 ggcaatatag ctcctccata catataccat agaagttaca tctttaaaga gcatttctct
     3001 ttttgatgca acttcttttt atattttgta gaataaaaag tttaatattt taaactcact
     3061 a
//
LOCUS       DAENCV010000243         3011 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.246, whole genome shotgun sequence.
ACCESSION   DAENCV010000243 DAENCV010000000
VERSION     DAENCV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.246"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            137..1030
                     /locus_tag="KQD82_003795"
     CDS             137..1030
                     /locus_tag="KQD82_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG4382229.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1473..1963
                     /locus_tag="KQD82_003796"
                     /pseudo
     CDS             1473..1963
                     /locus_tag="KQD82_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1991..2938
                     /locus_tag="KQD82_003797"
     CDS             1991..2938
                     /locus_tag="KQD82_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382230.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQKGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1319 a          259 c          424 g         1009 t
ORIGIN      
        1 atattataac aatagtagtg cttttaatta aatatatttc ataaatcttc ataaaatctt
       61 caaaatttcg taaagaatat gtcacaaaag taaggtatat tatattcagc agatagtata
      121 agggaggaca atatttatga agaataaaaa attcattgtt ataatactga taatatcaat
      181 tttaataggt gtattagtga aggaatattc atctagagag ataaaaaagg atgatataaa
      241 tgtcagtaag tatataaaat atgctgattt agcaagtaaa aataatgcac aagttaactg
      301 gaagtatgta gcttccattg ttgctgtgtt aaataaaaat aacttaaaaa acgttaagga
      361 ttcacagata caagaagtat cagatttatt tgttaagaat ttttctaaaa ataataaaat
      421 aaataagtta agtgatattt tggatgagtt agaatttagt aatagacaaa aaagattggt
      481 tgataattat atagacaatc ttaaggatta tggtataaaa ccagaaagat tgaagtctga
      541 tacaaaatat atgaagttta ttgatgaaat taaaacagaa gcaatacaaa attataagga
      601 ttataaaata cttccatcta taactattgc acaagctata atagaatcta gctggggcaa
      661 gtcaactttg gcaaaacaat ataacaatct atttggtata aaggctgacg cttattggaa
      721 aggtaagtca gtgactttag aaacgaaaga acatttagat acaattatag atgataaatt
      781 tagaatatat gatgataaaa atgaatctat aaaagaccat gcaaaatttt tagccacaaa
      841 taaaaggtat aaaaacaatg gtgtatttga tgcaaaaacg tatatatatc aagctaaagc
      901 attagaaaag gcaggttata gtactgcgaa ggatgaaaat ggcaatagta tatatgcagc
      961 tcgtctaata gaattaattc aacagtataa tcttcaatta atagacagtg aaattcaaag
     1021 tgaaatgtaa ctttgattaa atttttattg ataaggctat gatttttaaa atcatagcct
     1081 tattttgaca tgtttttact tttaattatt aaatcgatat cattatcttt caaaatttac
     1141 tttatactgt aaattaaatt atttaagtta aaacttggaa ttatttataa aaggattatt
     1201 tactttatct aaattctgca cttaaagaac atcttttccc ttaagactga ccgttagtct
     1261 ataaaaatta taattactta taaaaaagtg aatagagaga tatatatata attttcagaa
     1321 agacttttac ttgtaaattg aaattaatga agtaaaaaag gattctttct taaaagtaaa
     1381 taacaactag atagtgaata ataaaaaatg gtaatttgaa ataattttta tgaatagtat
     1441 taatatatag atacagggag attgatgcga ttatgtatga aaataataag gtaatataca
     1501 accttgaaag atgtgaaaaa ctagaaaaga ctctttattt atctcaattt aatacatata
     1561 aaaattttaa attagcttct aaaaaaactg taataaattc attagagaca tatgtttaca
     1621 atatgaatat taacaatata agtcttgttg atactataga atgtatatca ttagaagatg
     1681 aacaacttac aggaaagaag ttgattttag ttggtgatat agatattgat gcaatattag
     1741 attatgcagg aaataaaaga aatagaaatt ctagtaaaaa aatacattta aattaaaaat
     1801 accctttagt acatttatac aaatgcctag aaagatagaa aataaagata aaattaattt
     1861 aaagtatctt atacaagata ttacttcatc tattttagat gataatttat ttattagtgt
     1921 tactgcaatt ataagttatg aaaatagttc caagatagag taatctttaa attatagaaa
     1981 ggtggatgca atgagaataa cgaaaactac ttttaatgat gtattttcaa atgatgcaaa
     2041 tggaagtatt ataaaagtag aaaaatatgt tgataaaaaa tgtgcatctg tatttgagat
     2101 tttgacctac actattattg ttacaaatac aagtaggtac aaaactggaa atatattttt
     2161 caaagattat atttctaaat atatagagtt tataaataat actgtaaaag ttaatggcat
     2221 tataaaacgt ggattagacc cacagaaagg tttttacata gggagaatag atgcaagttg
     2281 taaaaaaata atatctttta agagtgtagt tttaccaaat tctgcacata ggattataaa
     2341 gaatagtgct aatatatact attattataa gtgtaatcta gataaattcc caacaagaat
     2401 atcaatagaa agtaatagag tttatactaa tgtaaataaa attgtattca aacaattgaa
     2461 tataagtaat actttaaaag ctcctaaaaa tctaagagat atattaaaag taaatgtaaa
     2521 tagtaaaata tttgatgtta aacctatgaa aaatcttata aacaaaggta gtaatttgaa
     2581 attatgtaac ttgatagttt ttgggtgtat agaagttgaa atagactata gttgtaaaaa
     2641 taggagtaat tttaaaacag taaataagtc taatgacatt aaaaaagata agtattataa
     2701 aaatgatata caaaataaca atgaaaatat aaacgattgt aaaaagcaaa taagttttgg
     2761 tgataataag gtaaaaaaaa taacaaaaac ttttggattt tcttgtttca tttgttctcc
     2821 agttggaatt atttatgaag atatgaaaaa tataaatata aaaattgaac atacatcaat
     2881 aaatgaatta aatccagaag aattatttgt aaatacatca cttttattgt attattaagt
     2941 attttaaagc taaaacttaa acttattaaa atagcatttg gttaaaggaa aatagatatt
     3001 gatatattta t
//
LOCUS       DAENCV010000244         2980 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.247, whole genome shotgun sequence.
ACCESSION   DAENCV010000244 DAENCV010000000
VERSION     DAENCV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.247"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            247..585
                     /locus_tag="KQD82_003798"
     CDS             247..585
                     /locus_tag="KQD82_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382231.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            607..924
                     /locus_tag="KQD82_003799"
     CDS             607..924
                     /locus_tag="KQD82_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382232.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1074..1751
                     /locus_tag="KQD82_003800"
     CDS             1074..1751
                     /locus_tag="KQD82_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382233.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2079..2846)
                     /locus_tag="KQD82_003801"
     CDS             complement(2079..2846)
                     /locus_tag="KQD82_003801"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG4382234.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1199 a          380 c          355 g         1046 t
ORIGIN      
        1 gctataatta acatattatt aattcgtttt attattgtag tatggctatg aaattatgaa
       61 aatatttctc taattaaaaa tatgatttat ccatatttgt ttattgtaga tacagactat
      121 acaaaaataa atagcactta tttcagtata aaaacatata tagtctatat ttagactaaa
      181 aaagtataaa tagtctatat aaaacaactt aaactatgaa tataatctaa agtggaggga
      241 taataattgg caaatatagg aaaaataata ggagatagaa taaaagtcta tagaacaaaa
      301 ttgggatata gtcaagagtt tctagcagaa aaggcaggtt tacatccaac atatattggt
      361 cagattgaaa gagctgagac aaatacatct atcaatatta taatgaaaat tgctactgct
      421 ttagacatac cacttgaatt gttatttgca aatattatta caacagaagg cgttgataac
      481 tctgtacctt tagaatgtta tgaaatgatt aataaattaa ctgaaaaaga acaaattgca
      541 atgctagagt taataaagtg catcattaaa tacaaagaaa tataagtgag ggaaatagta
      601 acaataatga attatatagg taaaagactt aaagaagaac ggaaaaaagc aaatttaact
      661 tcaaaagaat ttgcaaatat ggttggagta tcgccttggt atataactca aatcgaaagt
      721 ggtaaaaaga atccaagtct taaaacattc ataaaatttg taaatatatt aaatatttct
      781 gcagatgttc ttataaaaga tattacttcc actggaaaaa catatttaga aaatgatata
      841 aatgaagagt taaaagactt aaattcaaga gaactaaatc tgatatcaca gattatagac
      901 atcataaaaa agaataaaat ataattcttt atattttata taaaaaaaaa ggttatttga
      961 aataaataac ctttttttgt ctttttgtgt taacattgtc ttatagtgct aatgagtact
     1021 attagaccat gttctatggc cttattttaa tataaaacga ttggagatag gctatgattg
     1081 tgattgaagg tagcgataaa tttaagattg caaaagaata tattgatgta gaatatactc
     1141 tttttagcaa agtaactttt aggtatgaaa agttgaaatt taaagataat gctgaattgg
     1201 aaaaaattaa gatgtttaaa tataaaaatg gctacatccc taataaatta aacctttctt
     1261 ttggatatgg attctcttct tataaaaagc aaataattag agaaactgta gatactttaa
     1321 gattgacaga aattttttca agcgagaaca tagaagatat taaatttata aaagatggta
     1381 caaaaaaatt agaaattagc atagagaaag ttgtgaaatt taaacgtcga aaaaaaagaa
     1441 attatgtttg ttgctattgc cctgatatgt atagagacat aaaactcgac aaagaatcta
     1501 tcaataagat atacaacaga aaaataaaaa tagaaagaga agttaatatt tttgaagatg
     1561 aggatgttat aataaacaag aaagtattga agtttccaaa gtcttggaca aagaatatgc
     1621 aaaaatattg gttaagtgaa aataagtatc ccatacattc tactgtaatt gatgatgata
     1681 gatataaatg ttgtaatgta caatatacaa aaaatagagt gataatatta tattacatat
     1741 ataaccatta atatacataa ttacaaaaaa ataatgacaa atatacataa tacaaatata
     1801 tcttctactt tagataaaac agttattatg aaaacttact atctataaat aatagaaatg
     1861 ctagtatacc atctataatc taatgtctag taggttttaa tggcatattt taaatatgcc
     1921 atagttgatt tataagattt tctatatcac tttcaaaata cacactaaat agttgctttt
     1981 cagatataat tgcccaattt aaattttact atctgaaaag ttttttatat ttaatttata
     2041 tcttcaaaaa taccgtctaa tatttctata taattaattt aattaatact cttatgtaat
     2101 agattaagta ctgaatttaa ctctttaaat gatatttgcc ctggtgctga aacacttttt
     2161 gcagctccaa aagtcaatgc agaaccaaat atttcaccac acaatctact tataacaccc
     2221 attcctgaca ttgacattgt tattattggt ctatctgcat aaattttaaa catttcattt
     2281 gtagcttcaa gtagcactaa cacatctttt tcattttgcg gcataacagc tattttgggt
     2341 aaatctgcgc ccaattcctg cattctacat aaacgagata caatctcttc tttttttgga
     2401 gttttattaa aatcatgatt agatataatt actttcactt cttttttatg agcaaaatta
     2461 actacttcat ctatgacctc atctcccata aataactcta catctattaa atctactaaa
     2521 cctgtattag aaatttcttt attcaaagta gtataataat ctcttgatat taatttttct
     2581 cccccttcta ctacacttct aaatgtgaat aataaaggga tatcatgtat ataacttctt
     2641 aattcataga gaacttcctt aacttcttta atattttcta cattctcaaa aaaatcaact
     2701 cgccactcta taatatccaa acatgcatct tttaactctt tagcctcttt tataatatct
     2761 tttttatttt tcccaataat tggtacacaa attttaggtc taccttctcc aatagtaata
     2821 ttttttactt gaacttttct tttcatattc tcctccataa tcagaaattc tataggactt
     2881 taaattctat tttctgcaca atttaattat acattaaaaa tactatacat tataaaaata
     2941 ctaatttaaa aataattttt attctaattc ttaaaacaat
//
LOCUS       DAENCV010000245         2953 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.248, whole genome shotgun sequence.
ACCESSION   DAENCV010000245 DAENCV010000000
VERSION     DAENCV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.248"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            103..2460
                     /locus_tag="KQD82_003802"
     CDS             103..2460
                     /locus_tag="KQD82_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG4382235.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMVLAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2546..2863)
                     /locus_tag="KQD82_003803"
     CDS             complement(2546..2863)
                     /locus_tag="KQD82_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG4382236.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1115 a          329 c          485 g         1024 t
ORIGIN      
        1 aataaaatat aaaaattgag aattttataa tatatttgta gatttgagta taaaaaagtg
       61 ctaagttgaa aggagtaaaa gctaagttga aaggagtaaa atatggataa ggttaattta
      121 gaaaggtata gttgtgatat aaatgttggt ttaaataaaa atcaacttca aagtagaata
      181 gatgataatt taattaatgc atttgataat ggaaaaacca aaacatataa acaaattttt
      241 aaagacaata tatttacact ttttaatctc ataaatattg ttttagtctg tttattagtt
      301 tcagttggtt cttttaaaaa taccttattt atatttattg ctgtaattaa tactattata
      361 ggagtaatac aagagataag agctaaaaga ttattggaca atctatctgt tatagtttca
      421 aataaggtat ctgtcataag agaaaaagag aaaaaagaga ttgatgtaca agaattagta
      481 ttagatgata ttatgatttt aaaaacaggt aatcaaattt gtgcagattc aaaagtttta
      541 aatggaaaat tagaggtaaa tgagtccttg gttacaggtg aatctgatat tattataaaa
      601 aataaaggcg attttttata ttctggaagt tttgtagttt ctggagaagc ttttgtaaga
      661 gttgaaaata taggaaaaga taactatgca aataagatag ctaatgatgc aaagatttct
      721 aaaaaacata attctcaact tagaaattca ttaaatgcaa tattaaaaat tgtaggtata
      781 ataataattc ctctaggaat tatattgttt gcaaaacaac attttggaaa tggagattct
      841 atagctacat ctgtagtagg tacagtggca gctgtaaatg gtatgatacc agagggatta
      901 acattactta cgagcattgc tctagctgta ggaactataa aacttgctag tcataaaact
      961 ttagtgcaag aactgtactg tgtagagact cttgctagag ttgatacact atgtcttgat
     1021 aagacaggta caattacaga aggtaaaatg caagttgatg atatagtaat gttggaagaa
     1081 gtggatatta atgaaataat ggggaatata tgtaattctc tcaaagatga taatgcaaca
     1141 ctaaatgcaa taagagataa gtataatgtt ttagatacgt ataaagctaa gaatttaata
     1201 ccattttctt cagaaagaaa atacagtggg gttacctttg aaaataaagg tacatattta
     1261 ttaggtgcat ttgagtttat atttaaggaa aaaaataaaa aacttaggat agaagttgaa
     1321 agatattcta aaaaaggaaa cagagtaata gttttagctc atagtgattc ttatatggaa
     1381 gataataata ttcctgaaga tataaagcca ctagctttta tattgatatt ggataaaatt
     1441 agagatgaag ctaaggaaac tttagaattt ttttacaatg aaggtgtaga tataaaaatt
     1501 atatctggtg acaatcctat aacagtaagt gaggtagcta gaaaagcagg tcttaaaaca
     1561 gctagtaatt ttattgatgc aacaacactt aaaaatgata gtgatatata caaagcagtt
     1621 gataaataca gtgtttttgg aagggtaagc ccacaacaga aaaaacaaat gattttagca
     1681 ttaaaaaaac aaaatcatac agtagctatg actggagatg gtgtaaatga tgtgctagca
     1741 cttaaagaag cggattgtag cattgccatg gcatctggga gtgatgttac taagaatgtg
     1801 tctaatttag tcttattaga ttcaaacttt gcatcaatgc caaaggttgt tatggaggga
     1861 agaaaagtaa ttaataacat tcaaagagca tcttctttgt ttttagtaaa gacaattttt
     1921 tcattcttct tgtcgttatt aacattattc ttcttcagta gacagtatcc atttgttcct
     1981 atacaattat cactcattgg ggctgtaaca gtgggtatac catcattctt cttagctctt
     2041 gaagcaaaca aaagtagaat ttcaggaaat tttttactca atatattaaa aaatgcttta
     2101 ccaggagcac tatgtgtgac agtaaatgta attattgttt acaatatagt tgaatattta
     2161 ggttatagtt caagtgtatt ttccacaatg tgtgctatat taactggtgt atgtggtctt
     2221 ttaatattaa gacaacaatg tttgccattt aataaagaaa gattattttt aatagtaagt
     2281 atgactattg catttgttat tgggattcta tttttaggtg gattattctc atttgtagca
     2341 ttgagtagag aattaattct cataactatg gtattagcaa tattaatgcc aattatgata
     2401 aaagttatgt tatttgtctt agatgagata ttagagacaa ttgttccaga tttaaattaa
     2461 ttttaaaatg atataaataa gagcagtttt agttgaactg ctctttttag atgtgtttaa
     2521 ttttatgtaa gaatatgtat ttttattaat cagctatata tgagcaacag tagtcaacaa
     2581 cttcattaac ctttaagtca aaacttgaat cactaggaac attaaatttt tgcccttctt
     2641 tataagttac aaactcattg cttccaggca gttttacatc catacttcca gcaagcatct
     2701 ccattatttc ttcttctcta gtagaaaaag tgtattctcc tggcaacatt aatcctaaag
     2761 tttttctttc tccattaggc agtattatta ctctgctact taccttacca tcaaaataaa
     2821 cgtttgcctt ctttactgca gtaacattct taaattcact cattagataa aacctccaaa
     2881 attatttgta catatatatt atataaaaat aatgtaaaaa atgctatata tttacaaaaa
     2941 aatttagtat tta
//
LOCUS       DAENCV010000246         2945 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.249, whole genome shotgun sequence.
ACCESSION   DAENCV010000246 DAENCV010000000
VERSION     DAENCV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.249"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            49..555
                     /locus_tag="KQD82_003804"
     CDS             49..555
                     /locus_tag="KQD82_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG4382237.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            683..1699
                     /locus_tag="KQD82_003805"
     CDS             683..1699
                     /locus_tag="KQD82_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG4382238.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2052..2756
                     /locus_tag="KQD82_003806"
     CDS             2052..2756
                     /locus_tag="KQD82_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG4382239.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLGDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1241 a          276 c          523 g          905 t
ORIGIN      
        1 ataaatatag aatataaata tatccattta ataataagga gaagagatat gaaagagtat
       61 gtagttgtgg atttagaaac tacaggatta gatccatata aaggttgtga aataatagaa
      121 ataggaataa cagaaataaa aaatgaacaa attgtaaaaa actattcaag gttgataaaa
      181 cctaagggga taatttcttc atttataaca gaattgacac atataagtaa tgaaatggta
      241 gaaaatgaag aaccactaga attagtttta cctagattta gaaaatacat aggtgataga
      301 actataattg cacacaatgc gaaatttgat ttaaaatttt taaattacta tcttagaatg
      361 ttagatttag aaccaataaa caattatata tgtacagtag agttattaaa aaaatgtaaa
      421 agttataaag gaaaaaacaa gaaacttgaa acagcttgtg cttattacaa catagagaat
      481 ataaatgctc atagagctga cagtgataca ttagcaactg caaaactatt tttgaaaata
      541 aaagatgaat attaattata aatatagaga aaattagtga aaatatcctt tataaaattg
      601 acccttaagc ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct
      661 tatatgaaga agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag
      721 acgtaaaaaa attagtaaca acaaagatag taataaaaaa tctaaacatt cttatacttc
      781 ggcaaataaa aaaaataaaa gtcctaatac aagttccagt aaaagaatga gtgaaaaagt
      841 agattcacag aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc
      901 tttaaataca gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa
      961 aaaattaaaa agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gggcattatt
     1021 atctattatg ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc
     1081 agctataaaa gggtcaccag aagttactaa aaagttaatt aggtctaaat atataagtag
     1141 tgaggttgta agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttccataga
     1201 agatgagaga ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt
     1261 aaacaatgtg tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa
     1321 aaacctgctt actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc
     1381 taagcagatg aataagatta tgactaagga tgaaattctt gaagcatatt taaataatat
     1441 gtatctaggt aaaaatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa
     1501 ggttagtgaa ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc
     1561 aaggtatatt gaccatggag aggctaagaa gagacaagtg attgtgttgt ataagatgca
     1621 tcagcttggt tatataactg ataaggagta tagaatagca aaagctgatg acactccatt
     1681 taaatcagaa attgattaaa attgtaagaa tatagtattt caatagttta aacaaacgaa
     1741 aatgtaataa aaaaggtgat ttgccaccca tttgccacca taattataat ttaatagcaa
     1801 attgttttaa tgtacaaatc cgataatata atattgtttt aaatactaca ttatgctaaa
     1861 atttaattgt attttgtaaa ttaaaaaact tataatttag ttttctaggg ttccgcattt
     1921 tataatggtc aggtccaaga gaaaacgcac aattgttgtg tacacggaag gataaaagcc
     1981 tgggagatat ttaataatat ttctataggc ttttttattt ttacatattt taaatttgag
     2041 gaggttttct aatgaaaaaa tttgttattg aggatgcatt ttggaatcta tttcctagtg
     2101 caaaaattgg agttgttatt tgtaatgata tagataactc tataaaagat gaagactata
     2161 agaaaataat tttgaaggga gaagaagaag cattaaaata cttgggggac tcagaattta
     2221 gcaacaacca agtgattaaa gtatggagag aagcatttaa aaaatttaaa acgaaaaaag
     2281 gagcaaggtc ttctattgaa gcattattaa aaagagttca taatggaaat aatataggaa
     2341 ctattaaccc tttagtggat atctacaatt ttatctcatt aaaatatgca ttaccttgtg
     2401 gaggggagga tatagataaa tttataggag atattaggct aactagagca attggtaatg
     2461 aggagtttat cccactaggg acagatgaaa attcatttcc atatgaagga gaaataattt
     2521 ataaagatga tggtggagca atttgtagat gttggaattg gcgtgaagca gtgagaacaa
     2581 tgcttactga agatactaac aatgcctttt tatgcattga attagtagat gaaagtagat
     2641 ttaaagaatt tgaaaatgct ttaaatgaat tagctaaaat agttcaagaa aaattgggtg
     2701 ggaattgtaa aatttcaatt ttagatattg ataataaaga ggtatcagtg gattaatatg
     2761 gaggtgctgt tgtaatggac tattctagtt ataaagatac ttatatttag ggtaagaagg
     2821 tctattttag atataacgat tatcaaagga tataatatag aaaataaaaa tgtagaatat
     2881 aaaaagcctt taaaataaga aaaaaatgta ttttaaaggc ttttattata ctctttttta
     2941 tttgc
//
LOCUS       DAENCV010000247         2935 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.250, whole genome shotgun sequence.
ACCESSION   DAENCV010000247 DAENCV010000000
VERSION     DAENCV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.250"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            100..2328
                     /locus_tag="KQD82_003807"
     CDS             100..2328
                     /locus_tag="KQD82_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG4382240.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNNTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2566..2865)
                     /locus_tag="KQD82_003808"
     CDS             complement(2566..2865)
                     /locus_tag="KQD82_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG4382241.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
BASE COUNT         1099 a          397 c          395 g         1044 t
ORIGIN      
        1 attaagacaa aattttaact ttactatttg tgtaaaaagt aatataattt aaaattgatt
       61 ataaatctaa cttttttctt tatcaaaata ttttaggagg tgagacattt gaaaaaaact
      121 aatagtttaa aagaaatttt aaactggaat aatatcaaga atttaagacc ttcaaagggt
      181 ttaaaaaata tattagaaat ttttaaaagt gacctaaaag atatacgtgg caattatgcc
      241 gtacttgtaa tagttttagg tcttgctttt ttaccatctc tgtatgcttg gttcaatata
      301 aaggcatctt gggaccctta tggaagcact gctaatatgc ttgtagcagt aacaaatgaa
      361 gataaaggaa atgaaatagc aggacaaaag attaatgtag gagaccaatt aattgaacaa
      421 ctcaaagata acaagagttt aggttggcag tttgtagatg aaaaaacggc tcttgaaggt
      481 gttaaaaaag gtacttatta tgcatcactt aagattccaa aaaattttac aaaagatttg
      541 acatctttaa ttacagatga tgttaaaaaa ggggaaatta tctatactgt taatgaaaaa
      601 ataaatgcag ttgcaccaaa gattacagat aaaggtgctt ctacaataca actacaggtt
      661 aatcagacta tagtaaaaac agttagtgaa atcgttcttg gtatatcaaa cggagttggt
      721 gcaggaattg aacacagtct tccaaaattg acaaaagttg aaaattccct tattgacctt
      781 caaaagagat ttgaccatat aaataagact gttgatttag cttctgatgc tacttcaaaa
      841 ttatctgata cagttaaaga tattaaatct gatttaccta caattaaaaa aactctgaat
      901 gatacaaaac tgttatcaag tgacttaaaa aaattcttag aggatacaaa tgataattta
      961 gacgaacttt ctcctcttat taagtctgac ttaaatttaa tggttgacct ttcatcttct
     1021 gcttcttctc ttacattaaa tttaattgat gcagtaaata gtggcagtga agatgtacct
     1081 aagttgatag ataatttatc agaaaaactt tctaatttgc aatctttaaa taatactttg
     1141 gtggattttt taactaaatt aaaccaactt acatcaaata atagattgga tgatgtcata
     1201 gataatcttg aagactctag caataaaata gattcatcta tatcaacatt aaatgatatt
     1261 aaaaacaaag ttatatctgg acaacaacca tctataagtg ctttgaataa tgttttaagt
     1321 ctatcaaatg gtattggaag aattaattta aatattttaa ataattttga ctcaaaaata
     1381 tcaaaaccta taaatagtat atttgcaaac agtataaaag tagcaaatga tataattact
     1441 gttttagata aggcagaagc aaaacttcca aaggttgaag aaatacttac tacttctcta
     1501 aaactttcag gaaatgcaca ggaaagtata tcattgataa gagaaagact tcctttagca
     1561 aaaggaatgt tggatgattt gattgatact ttaagcaaaa ttagtaatgg tgaagatatg
     1621 aaaaaattag tcagcttgct tgaaagtgat atattgacaa agtctaattt cttaaaagaa
     1681 cctgttgagg tgaaaacaga gaaattatat ccaattgaaa attatggttc tgcaatgaca
     1741 ccattttata gtgttctttc tacatgggtt ggtatattac ttatttcagc actattgtct
     1801 acatctgtac atggaaacta taaaccttat gaaatttact ttggaagagg ccttactttt
     1861 ttaagtatag ctcttgtcca aggttttata ataggaactg gagatatatt attgttagga
     1921 gtaacagcaa aacatccaat tttattgata gttctgctta tgtttactag tatggtattt
     1981 aactttatag tgtattcttt agtttctgta tttggaaatg taggaaaagc aattgcaata
     2041 gtactattag tcgttcagat tggttcttct ggtggtacat tccctataca agttacacct
     2101 acattcttcc aaagaataaa ccctataatg ccatttacct atgcaatagg tgctacaagg
     2161 gaagcagttg gtggtatata tgctccaaat ctaactaaag atatactagt tctattacta
     2221 tttatgattc tgtctatttg tttaaatgta cttctaaaag gacctataaa tagagttata
     2281 aaaccattta atgataaatt tggcgatagt aatttaactg gtcattaaaa aattaattct
     2341 gtctttaaat atattttttt aataatattc taagttcata ttaaaaggta gtagctaaaa
     2401 agctactacc tttttccaac ttaaaaacta atatttacta caaaaactgt catagaaaaa
     2461 aacgaaaaaa ttcgttcttt cctaaattta tcttataaat atttagatat attcctactt
     2521 aataagcaga gctatacata gtatttaatg tataacttac attatttaac tcatataatc
     2581 aattattcct gaatttaaat tatataaatg ctcaaaacct tcttcagcta acaaattaca
     2641 tgcagtaaca cttctatgac cacttctgca ataaactact actttcttat ctttataatc
     2701 gtaaatttca tctatattat tgattaactc ttgtaaagat atatttatag cattttctat
     2761 tttatattcc ttaaattcat ctttagttct tacatctaat aataaaacat ccttttcatt
     2821 tttcatcatt tctttaagtt gcttcttatt tatattctta tacatatgtc taaaattcct
     2881 ccattttatt tatatattac ctataccctt tattctttat acaacaacaa atatt
//
LOCUS       DAENCV010000248         2920 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.251, whole genome shotgun sequence.
ACCESSION   DAENCV010000248 DAENCV010000000
VERSION     DAENCV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.251"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            418..2229
                     /locus_tag="KQD82_003809"
     CDS             418..2229
                     /locus_tag="KQD82_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG4382242.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAVLSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQIENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2317..2898)
                     /locus_tag="KQD82_003810"
     CDS             complement(2317..2898)
                     /locus_tag="KQD82_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382243.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     YSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1106 a          297 c          413 g         1104 t
ORIGIN      
        1 aaaggaatat atttacataa tatgataaaa taaattcaaa gagttaaatt gtaggtaaat
       61 agtattttaa ttatttatta aaatatagat tatgtatatg ccaaatttaa aaagagacat
      121 aaagacatta tacaaatata ttattggggt gatattttat aagaaaagaa gtgatgcagt
      181 aaattaattg agcaatttaa aaatgattta atgatagtgt tgatgaatgt ttaaaaagaa
      241 attttagaaa aacgtatatt gctgtttgtg ttggaggtga tgaatttgta taatttatag
      301 gatataaatg attaaactat ttaagataaa tacatatcac tgtaaaatat tgataatcat
      361 tttttataag agattgatta tttgcaaatt atatatatta caaatgaggt aacaataatg
      421 aagaaaaaat atgatttttt aatttattct gctatttggc tagttattat tattcttttt
      481 atattttctc tatatcatag tatagagcat attaaaatta taagtcacac tggtactatt
      541 cgcagtgaaa ctcaaaaggt tgttaagcaa gaattgaata atgaaagaaa tgatgacttg
      601 ataaaacgac tggataatat tctgataaag ctaagaacag gtaatggaga aaatggattt
      661 caaagatgtg ataataaaga atttcaacaa aaactaaatc aaatggattc tatgtgggaa
      721 tctatgaaga aagaaattat aaaagttaga aatggtgctt caggtgataa attatataaa
      781 ttgagtgaag agtattctgt gttgtctaat caaattgttt ttatatctga aaaacactct
      841 aatgcaaaat tgtattcttt tgctactgca ctatttattt atcttatatt ctcaacaata
      901 agtcttttaa tttgggaata ttataataaa aaaaggttta aaagaatatt ttatactgat
      961 aatttaacaa aaataaaaaa tcaggttgca tttgaaaaca gagcgattga aattttgcac
     1021 aatgcatcta acaaagagta tgtactgcta aatattgata ttgacaattt taaatatatt
     1081 aatgatacac atggatatga atatggagat aaagttctaa taatagttgc agctgtactt
     1141 tctaaaactt ttaatatcaa agaaacttgt gcaagaattg gttcagataa ttttgtaata
     1201 cttgcaaaat atagagattc tcttttagag gatattcggg aaatgctaac taatgctata
     1261 atctctgaat tagatatgaa tgtaactcaa actatatctt attgtatagg agcgtactta
     1321 gttgaaattg ataatttagg ttataaatcc atcaattcta taatggataa ggcaaatatt
     1381 gcacacaagg taagtaaaac aagaggaatt tcctccacag tatggtataa tgaaaatctg
     1441 ttaaaacagt tacaaataga aaatagtatt tataattata tgtataaagc gatagaaaat
     1501 gaagaatttc atatgtattt gcaacctaaa tttcaaatat cttctttaaa tgttgtgagc
     1561 gcagaagctc ttgtaagatg gttttctcca gaacttggtt ttttatcacc agatgaattt
     1621 attccattgt ttgaaaaaag tggatttatt attgaattgg acttttatat gttaaaaaaa
     1681 gcgtgttcat ttgtaagaaa aacgtttatg aaaaaaaatc aatatacata tccgattgca
     1741 gttaattttt caagggttac tatttaccaa aatagttttt atcaaaggtt tttagatact
     1801 gttaaagaat atgaaattcc ttttaagtat atagagatag aggtaactga aagtgctttt
     1861 aatgaaatat ctcaaccagt tatttcgatt ttagaggagc ttaaaaagtt aggattttta
     1921 atttcaatgg atgattttgg atcaggatac tcttcattaa gtcttttatg ttctctatct
     1981 attaatggat tgaagttaga taaaagctta cttaaagaaa catttaaccg ggagaaagta
     2041 tatagtatta ttcagtgtat tattgaaatg tcacatcgta taggcatgtc tgttgtatgt
     2101 gaaggtattg aaactaaaaa agacttggaa tttttaaata cagttaaatg tgatgtagga
     2161 caagggtttt atttttctaa accgatagaa gaaaaagaat tttttaacaa atatgtaact
     2221 aaaaaataat ataattattt ttatcagtct atacattgag tcattttata tctcgttaaa
     2281 tgactcaatg tattgttatg tattttaaaa tataaattat ttaaaacgtt ttttgtactt
     2341 atatagaaat tcttctatat tttttgattg ttctcttatt tcaactgctt ctatatctag
     2401 taaataaagt atagaataat ttgtaagttc aacatattca ccatcttctg aaccgagtgc
     2461 atgtctctca atttttaatc ctttttcttc ataatatttt tttgtttcta tatatagtga
     2521 ttctcgcttt tgtatgtagt ctttcacttc attatttgga aagttatatg aatccactaa
     2581 tgtttcgcca tttataaaag ttttctcggt agcaactttt tcatcccaac aatggatttc
     2641 ttcccaagta aaaaatatct cagttccatc tggaatgaat gaaaacctgg taataatata
     2701 taaataatta ttgaaattta cttcataata ttcaggaata tcttcaaatt ccatatagta
     2761 atttgtataa ttaaaattac tattatcttt ttttacaaag ggtatttttt caataaattt
     2821 atatattgat tgttcatttt taaaaattcc tatatcataa cttttttcaa attgattaat
     2881 ttttaataaa tacaacattc tttctccttt tattcaaaat
//
LOCUS       DAENCV010000249         2868 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.252, whole genome shotgun sequence.
ACCESSION   DAENCV010000249 DAENCV010000000
VERSION     DAENCV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.252"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(16..462)
                     /locus_tag="KQD82_003811"
     CDS             complement(16..462)
                     /locus_tag="KQD82_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4382244.1"
                     /translation="MIKFEKLNIINNKDMKEILDTWESSVRATHDFLSEEKIISIKPQ
                     VKEGAKYVSNFLCVRDKKGLIKAFMGIHDFKIEMLFVGNYSIGNGIGKRLAEYAIEVL
                     NVNYVDVNEQNQQALGFYKHMGFKVFKKSEFDEQGNPFPILHMKLK"
     gene            1232..2071
                     /locus_tag="KQD82_003812"
     CDS             1232..2071
                     /locus_tag="KQD82_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382245.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            2061..2504
                     /locus_tag="KQD82_003813"
     CDS             2061..2504
                     /locus_tag="KQD82_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382246.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            2515..>2868
                     /locus_tag="KQD82_003814"
     CDS             2515..>2868
                     /locus_tag="KQD82_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382247.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLL"
BASE COUNT         1162 a          253 c          435 g         1018 t
ORIGIN      
        1 ctatagttta tattgttatt ttaatttcat atgcagtata gggaatggat taccttgttc
       61 atcgaactca gattttttaa agactttaaa tcccatatgt ttataaaagc ctaatgcttg
      121 ttgattttgc tcattcacat ctacataatt tacatttaat acttctattg catattctgc
      181 aagtcttttt ccaattccat ttcctatact ataattacct acaaataaca tttcaatttt
      241 aaaatcatgt attcccataa aagcttttat aagacctttt ttgtctctta cacataaaaa
      301 attactaaca tatttagcac cttctttaac ttgaggcttt attgatatta ttttttcttc
      361 acttaaaaag tcatgtgttg ctcttactga tgattcccat gtatctagta tttctttcat
      421 atctttattg ttaataatat ttaacttttc aaattttatc atttaataat tcctcttaag
      481 taagtatgtt acttaatatt atatgttaaa tatgatttaa aaaggttata agaatagttt
      541 tgtattaaat ataatcaaat aaatatattt atttgtcata aattaaatgt ccactaaaat
      601 agaaaaaata agaagaaata ggtaataaat gatagcttat agcaaaataa agatgtaagt
      661 ataacaatag atgagattgt aattaagtaa ctatacctaa cagaacattc ttagacgaat
      721 atagaagact taaaaaagaa aagagaagta gataagaatt tattaatgat tacatataaa
      781 ataaatgaat tataatttta ttaatgatat aataataatt aacaaaaaat aaaagaggag
      841 cgagttaatg tttgatggca tacactcatt ttctaaccaa tgaaacaata gattcaatta
      901 aggttactaa agtctaatta acacataaac atataggata aatacatctt tttaatatat
      961 taattatgtt agacttacaa aaaatgtaaa tatatagctt gttaaatctt gattgaacct
     1021 taaataataa gggataaaaa atgacttgtt caaaggcaaa gaatatacta ttaaatgata
     1081 taagtaactt agagataata tgtagtaagt caaaaggact atatagcatc agtaagtaaa
     1141 aagagtaaaa acttacataa tgcactataa aataaatgag ttagaaaata aatataattt
     1201 atataataaa gtagtacaaa aaggagtatt tatgtttgga tatgttaaaa taaacaaaat
     1261 ggatttaaca tttagagaat atgattatta caaagcttac tattgtggac tatgtaaata
     1321 cttaaaaaga aatcatggag aaatctctag gttttctttg aactatgata taacattttt
     1381 aattgtttta ttaacagcag tatataaccc agagtctata agtacagagg aagtgtgtat
     1441 agtaaatcca tttaaaaaga aaaaggtaat tacaaatgat attacagaat atgcagcaag
     1501 tatgaatatt cttttaacgt attataaatt ggaagataat ttaatggatg ataaacgcat
     1561 aaaagataaa ttggcatatt atatatataa aaacaaatta aaattagcat atgaaaagta
     1621 tcctgaaaaa gcagagtata taaaacaaca actaaatgaa ttaaataagt tagaaaaaga
     1681 taaaaatata aatatagatg aagtatctag tatatttgga aatataatgg gtgaagtatt
     1741 tgtctataaa aaagatgaaa atgagagaaa cttaagaatg ataggtttta atataggtaa
     1801 gtatatatat ctactagatg cctatgaaga tttagatgaa gattttaaga aaggaagata
     1861 taatccattt atagagtata ttgataaaaa tgatgagtta aaagaaaaag taaaaaaaat
     1921 aatagtaaca tcattaggat ttttagctag gggaattgat aatctaaatc taaataaaaa
     1981 tgttggaata atagaaaata taatttattc tggtgtttat ttaagatata ttaatatatt
     2041 agaaagtaga ggtggaaaga atgtacagta atacttataa taatgaaaat tttatgagag
     2101 caagatcatt catagaaaat agtgaattta aaaaagcata tgatttttta aaaactttga
     2161 ctgacaaatg tgcagagtgg tattatttaa ctggtttttc tgcaatgaac ataggatatt
     2221 atgaagaagg tgaagacttc ttaaaaagag caaaatttat ggagcctgaa aatagtgaat
     2281 atagtgatgc acttagaagt tatacacaat atagaaatga ttatagcaat agggctgaca
     2341 attataatag aagaagacgt aatgatttag atggatgttg ttgctgttgc tgtgatgatt
     2401 gctgttgttg tctaggtgac gattgttgtg aaaattgtgc taagctttgg tgtttagatt
     2461 catgttgtga atgttttggt ggagatttaa taacttgttg ttaggatgat aaatatgagt
     2521 ataaaagttg cttatggagg agttttatta gcattaaatg tgatattgtt gactcttaca
     2581 aatattattc ctgtaaatac attgtttatt atggggttag catcactttt agtatcaatt
     2641 gtaataatgg aatggggctt taagagtgga atcgcttttt atataggttc tatagtttta
     2701 ggatttatag tgatggcaag taagtctcaa tggattgtat attcacttac ctttggaata
     2761 tatggtattg taaaatatct tatagaaaaa gataggtcta tatatattga gtattttatg
     2821 aaattagtat ttgcaaatat aatgatactt atattgtatt ttttatta
//
LOCUS       DAENCV010000250         2821 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.253, whole genome shotgun sequence.
ACCESSION   DAENCV010000250 DAENCV010000000
VERSION     DAENCV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.253"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            221..1144
                     /locus_tag="KQD82_003815"
     CDS             221..1144
                     /locus_tag="KQD82_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG4382248.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIESDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SATYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNILKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1119..1946
                     /gene="panB"
                     /locus_tag="KQD82_003816"
     CDS             1119..1946
                     /gene="panB"
                     /locus_tag="KQD82_003816"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG4382249.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1965..2813
                     /locus_tag="KQD82_003817"
     CDS             1965..2813
                     /locus_tag="KQD82_003817"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG4382250.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1122 a          333 c          459 g          907 t
ORIGIN      
        1 cataattttt ttactgatat tttattatat aaattttata gaattaattg tcaatttttt
       61 atgaaaatgg taagatattt atggatggta tcttgttata agaactatac tttaaatgtt
      121 tttaattatt ttgtcttatg taactcatat cctcaggaat gagattagaa attaagatgt
      181 tgtatcacaa agtggaggtg atactattaa aataggcttt attggttctg gaaaagttgg
      241 aacatctttg gcaaattatt ttttatcaaa aggttattgt gtttcaggct tttatggaag
      301 gaatcaatta tctttattag aatcaacaaa tttaacaaaa acaaagattt acagcaattt
      361 gaaagatata atttatgaaa acgatatatt atttattaca acatcagatg attctattga
      421 aattatagac aaaaaacttt ccaaatttaa tttaaaacat aaatacattt gccacacaag
      481 tggctcttta aaatctagta tattgttctg ttcaaaaaaa gcaggtgcat taatttattc
      541 tatccatcct atatttgcat tttccaataa aaataccaac ataaaaaaaa tgaaagacat
      601 ttgtttttcc atagaaagtg ataatgaaca agatgattta gttattcaaa atttcataga
      661 tgatttggga aaccaatttt ttataagaga taaggatact tcagccactt atcatttagc
      721 taatgtttta gtttcaaatt tagtattatc tttactagat ataggagtaa gttactttat
      781 taaattaggt ttatctgaag aggaatcttt aaaagctatc agtcctttag taaaaaaaaa
      841 tatagaaaat atacttgata atggctttac taaagctctt actggtcctg tttctcgtgg
      901 agatataaca ccaataagaa aacatctatc tgtattggat aaaaaagatg aggatattta
      961 caaaatacta tccttaaaca tactaaaaat aattgcctta ggaaatgaca attcattaaa
     1021 aggtataaaa aatattacta tagaaaatgc aatcgaaaat ttaataagta aatctgaaaa
     1081 atacaaagaa atttacaaac ttttaggagg aaaaaacgat gaaaaatacc atacagactt
     1141 ttaagaatgc taaatatgaa ggaaaaaaac tatctatgct tactgcttat gactactcaa
     1201 ttgcaaaaat aatggatgag tgtgatataa atggtatttt aataggagat tcattaggaa
     1261 tggttataaa aggtgaagaa aacactctat ctgttaccat agacgaaatt atatatcata
     1321 caaaagctgt aaaaaatggt gttaaaaatg ccttaatcgt aagtgatatg ccttttctat
     1381 cttaccatgt ttctatagaa gatgcagtta aaaacgccgg tagacttata aaagaaggag
     1441 gagctcatgc tgtaaagtta gaaggtggct caaacgttat aaagcaaata gaaagcattg
     1501 taaatgctca aatacctgta atggggcatt taggattaac tcctcaatca gtaaattcat
     1561 ttggtgggtt taaggttcaa ggaaatacca gtgaaacagc aagacaactt attgaagatg
     1621 ctaaattaat agaaaaagca ggtgcattct ctattgtcct tgaaggagtg cctacgaaaa
     1681 tagctgagat ggtaactaat tctatttcaa ttcctacaat aggtataggg gcaggaatta
     1741 actgtgatgg acaaatttta gtttatcaag atatgttggg aatgtttgga gattttgttc
     1801 ctaagtttgt aaagcagtat gcaaatatag gagatataat gaaagattca attaaaaatt
     1861 acatcctaga agttaataca ggtgcatttc cacaagaaaa acacagcttt tcaataaatg
     1921 aatctgagtt agaaaaatta tactaatatt aaggatggta caaaatgctt gtaaaagaaa
     1981 ttaaattact aagaaatata atcaaagatt ggagaaaaca tggctattct atagggcttg
     2041 ttacaacaat gggattttta catgaaggac accaaagtct tataaaaaaa gctataaaag
     2101 aaaatgataa agtagtagtg agtgtatttg taaatccaac tcaatttgga ccaaatgaag
     2161 attttaatag ctatccaaga gatatagata aagattttaa atattgtatg gatagtggag
     2221 caacagtagt ctttaaccca tcaccagaag aaatgtattt aaaaggtaat tgtactacca
     2281 ttaatgtttc tggacttaca gattttcttt gtggagctaa aagacctgtt cattttggag
     2341 gtgtctgcct agttgtttca aagtttctaa atatagtaac acctgataaa gcttattttg
     2401 gagagaagga tgcccaacaa ttagctgtaa ttaaaagaat ggtcaaggat ttaaacatag
     2461 atacagaaat aataggctgt cctataattc gtgaaaatga tggattagca aaaagttcga
     2521 gaaatacata tctctctgag gaagaacgaa aatctgctct aatattaaat aagagcttaa
     2581 gtttagcaaa agaagaatta gtaaaaggca atctaaatcc agagaatata aaagaattaa
     2641 taacagctaa aataaattct gaacacttag caaaaataga ctatgttgaa attgtagata
     2701 gtgagacatt acaacctgtt aagcaaatag aacattctat acttgtggca attgctgtgt
     2761 ttataggaaa aaccagacta attgataatt ttacatttga gttaaatata taatctataa
     2821 t
//
LOCUS       DAENCV010000251         2815 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.254, whole genome shotgun sequence.
ACCESSION   DAENCV010000251 DAENCV010000000
VERSION     DAENCV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.254"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            19..693
                     /locus_tag="KQD82_003818"
     CDS             19..693
                     /locus_tag="KQD82_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG4382251.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      1048..1144
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1198..1374
                     /locus_tag="KQD82_003819"
     CDS             1198..1374
                     /locus_tag="KQD82_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382252.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(1561..1800)
                     /locus_tag="KQD82_003820"
     CDS             complement(1561..1800)
                     /locus_tag="KQD82_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382253.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1945..2754)
                     /locus_tag="KQD82_003821"
     CDS             complement(1945..2754)
                     /locus_tag="KQD82_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4382254.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT          961 a          420 c          331 g         1103 t
ORIGIN      
        1 gaagggggtg ctagataaat gaaccatcat atattgattc aatttgaata tatagcaaga
       61 atcattactg ctggtttctg tggggcttta attggatatg agagaaagaa tagactaaaa
      121 gaagctggaa tacgcactca ttttatagtg gcattagctt cagcacttat aatcattgtt
      181 tccaagtatg gatttaatga tatagtgggt actccaggta ttggtctaga cccagcccgc
      241 gttgcagcac aagttgtcag tggtataggt tttttaggtg ctggattaat atttattaga
      301 aatcaatcag taagtggtct tactacagct gctggtatat gggcaacagc cggtacaggt
      361 cttgccattg gctctggatt atacttagtt ggtattgttt caagtatcat tattgttatt
      421 gtacagataa cacttcatag agataggaaa tggatgaaac tccctacagc agagcaagtt
      481 tctcttaaaa tttctaacag cactgattcc atagaatata ttgaaggtaa atttaaaaaa
      541 tctaatattg aaattataaa catgaatatt aaacgccttg atggggattg gttagaagta
      601 gttatatata ctaagttacc taaaggatat aaaaaaacaa atttattaaa attatttagc
      661 gataatcctc ttatagaaac tttagaaata taatataaaa attgaagctt aaaaataata
      721 atatatttaa tcaaggtcaa tatataaata attacgggat gagtctatat aaaattcatc
      781 ccttgttttt tgccattcat atatattttt tcaagatatt tttgttcatc tattatctag
      841 ccaagtaacc cttgtgaact aaacttttta atataaaaac ttcaatacaa aactaaatat
      901 aatttttatt aaacattcta tacatatagt ttgtttcttg agtgaaaaat agatttcata
      961 ttgttaagat attgttaaat atttcaattt tctgacaagt atgttgaaca ataacttatc
     1021 ctagtgtatt attttagttg tagaataaaa ctaaatcggc aaaactagag aaatttagtg
     1081 acgcaaagct atagggacta acgcttataa aataaactat gagttatgtc agccagttgc
     1141 caaaaagata ttgttctttt tgttttatga aagtttttta gggggatagt aatatatatg
     1201 tttagagatg aaatggataa atgtacacac atgttaactg cttatattag tagtttatat
     1261 gattattgtg attttataga tacacagcta gatgatttta tactagagta cggagaaaat
     1321 gtagtagaat cttgtttaca tcaagtgatg gtattggtaa gtaagtataa ttaaatagaa
     1381 attgttttct agagaaccct ttttagtttt atctacattt aaaaatgtta ttatattaaa
     1441 aaactaatct cccctatatg caaagtttct tccatacaac aatttcttcc catttatact
     1501 ttttcacttg tatctttaat aaaattcaac ttcaaatatt aatatacttc aaaaattatt
     1561 ctatttctta tctttacacc aattcctatc agtattatcc tttatatagg ttctaactat
     1621 ttctccacaa tctaaacata aatctgaata tatagtagat tgtactggaa aagttttatt
     1681 tataacttta tctaattttc catctcctct atattttaat ccaatagttt cagcatatat
     1741 gccaccagtt gttaaaattc ccttttcaat atttacactt ccacaattag aacatttcac
     1801 atttataacc ccctaaacaa ttattttttc atgctatctt ataatttaaa atataatttg
     1861 ctaccgattt tataacacag tgacaaatcg gtggcaaatc aacatttttt cattctattt
     1921 accatctaat ccattgaaat atagctattt agttatagga acttgaattt ccgttacaaa
     1981 ctcctctaca attccagtct catgtatatc tatcttgtag atttctatag ggtcagcaat
     2041 tatcttataa ttgttttctt ctataaattt aaacaccttc tccatataca tcttattatt
     2101 agaattactc ccagtataat ttaaagttac ataatatcct tcatcaaaaa ctatattata
     2161 tactttctca ttactttcca gaaaacaaaa tacagattta aactcattaa atataccttt
     2221 attaattgct tcagtagaaa aggcagaacc aatattgtta tttccaagta tattaaatct
     2281 atcttcatat tctttctgaa gtttttgaat taaaaaatcg aagtcttcat ccctctttat
     2341 atcagcattt aattctaaag cttttctttt ttctatatat acaacctcta tctcatctat
     2401 ttttgtattt tgaattacac tttcaattga actcaatctt tttataatat tttccttatg
     2461 gcttataaat tcagctattt ttttatcaat taaatcgagt tcttcattta aaatattttt
     2521 cgtagactct atacttctgt catctaggta ttcttttatt ttcttcattg gaaagttcaa
     2581 gcttctaaat tcttttatta aatttaattt ccatatatct gatatattat acattctata
     2641 tccatttata tctctaactg gtcttagaat gcctatttct tcataataca ttaatgaatc
     2701 tctacctatt ccatatattt tagatatctc tcctatttta tagtaatctt tcatttatat
     2761 aataaactcc tccgaaatct tatttttatt ttaaatcaac attattactc tataa
//
LOCUS       DAENCV010000252         2798 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.255, whole genome shotgun sequence.
ACCESSION   DAENCV010000252 DAENCV010000000
VERSION     DAENCV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.255"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            343..1497
                     /locus_tag="KQD82_003822"
     CDS             343..1497
                     /locus_tag="KQD82_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG4382255.1"
                     /translation="MSNNINQNLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            1548..>2798
                     /locus_tag="KQD82_003823"
     CDS             1548..>2798
                     /locus_tag="KQD82_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG4382256.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
BASE COUNT         1043 a          309 c          518 g          928 t
ORIGIN      
        1 gaaaaatcta tttatgataa aaaaactaat gcttatatgt aattatttat attgtgatat
       61 aaaaataggt atatcttagg atgactttag ttattctaag atatacctat ttttgtattg
      121 actattttaa acattgtaaa aaaaatatca aaaacatatt gactctatag ttactataga
      181 gtttataatt agaactgtaa aaaaacaaaa taagtgtcat atttttagaa attataacaa
      241 ataaaaaagt atacttgaaa aaatgtatta agactaagct aaattgatga aatttggcag
      301 ggaaaacgtt gtataaataa gtcaaaatgg gaggaaatca aaatgagtaa caatattaat
      361 caaaatttag agacaaaaat aatacattgg ggacatagtg cagacccaac aacaggtgca
      421 ttggctacac caatatgtca aacagcaaca tttgctgcaa agacagtaga gcattttgaa
      481 gagttgtgta tgacatgggg atatgtgtat acaagagagt gtaatcctac tcttacagag
      541 ttagaagcta agttggctat gcttgaaaat gcagaaagtg ccatatctag tacatcaggg
      601 atgggggcaa taacctctac tatattagca ttggtaaaaa gtggtgacca tatagttagt
      661 tcagatggaa tattttcaca cactaaatta ttcatgtctg agttgttatc taaatttgga
      721 gttgaagtta catttgtaga tgcagtaaat ccacaaaatg taaaagaagc aatgagacca
      781 aatactaaaa tagtttatat agaaagtcct ttaaatccat cattagactt agtagatata
      841 aaaacaatag cagaaattgc tcatgaaaat aaaagcttag ctatagttga tagtactttt
      901 gggacaccaa ttgtacaaag accaatagat ttaggtgcag atttagtaat acatagtctt
      961 actaagttta taaatggaca tggggatact ttagggggag cagtagcagg ttcaaaagaa
     1021 ttaatagatt tagttagatg gccaagttta tgttgtttta caggagcatc tttaccacca
     1081 atgagtgcat ggatgatttt aaggggtatg aaaacgttag atatgaggat gaaaaagcat
     1141 tgtgaaaatg gattagcagt agcagaattt ttagaggaag aagagaatgt ggaattggta
     1201 aagtatccag cattaaaatc tcatcctcaa tatgagcttt gtaaaactca aatgaatggt
     1261 ttaggtggag gtgtagtttc atttaaactt aaggatggta tcaatggatt gacaagagac
     1321 caagctagta gaagattaat gaattcttta gagcttgcaa caatagcaac aagcttaggg
     1381 gaagagcata cattagttca aatgaatggg gaaaatctaa taagaatagc tgtaggatta
     1441 gaatcttcaa atgatataat aaatgatttt aaacaagcaa tagaaaaatt aaaataaaat
     1501 tatatgaatg agattataaa caataaaatt ttaaggagag agaatcaatg gaaagcaaaa
     1561 ataatgttaa aagatttttg ataatattca tattggcatt tggaacaaca gctatgtata
     1621 gtttaccata tatgaaatca tcattttatg acccaatgca acaagcttta gcattaagtc
     1681 atacacaaat agggaactta ttgagtttat atggattagt aggaatggta tcttatttta
     1741 taggaggatg gtttgcagat agattctcag ttagaaaatt aataactttt tctttaattg
     1801 cttcaggaat acttggattt tatttctcta cattcccatc atatagtatg atacttttaa
     1861 tatttgtatt atggggattt acgacaatat taaccttctt ctcagcctct gttaaggttg
     1921 ttagaatgca aggaagtgag tcagagcaag gaagaatatt tggtttttat gaagggttat
     1981 caggcgtatc aggtacatta atttctttta taggtcttta tttctttggt aaatttgctg
     2041 aaataacagt aggattcaaa tatgtagtat ggttatattc tgcagcatct ataatatgtg
     2101 gtatacttct atttttctta gtagaagaga aaaaagatag tggagcatca gatgaaggat
     2161 taagtattaa gtcattgtta aaagtagtta caatgccaaa ggcttggtta attggtctta
     2221 taatattttc aacttattta gtattctcaa gtttaactta tttaagtcca tacttatcag
     2281 aagtttatgt aatgccaatg actctagttt ctgctttatc aataataaga acttatgtta
     2341 taaaaatggg agcatctcca gttgctggtg ttataacaga taaagttggt tcatctataa
     2401 gagtaatgtt tgtaggattt atattaatga cagtaagtac agctgcttac ttggttattc
     2461 ctaagagtac aggatttata tggatagctg taataaacat gataatacta agtgtaattt
     2521 tatttggatt tagaggtata tactttgcat ctgtttcaga gtctaatata tctttagaaa
     2581 ctacaggagc agtagttgga tttgcttcat ttataggatt ttctccagat gcgttttatt
     2641 acactattgc aggtaattgg ttagataaat atggacaaac aggatataca tatatattta
     2701 ttttatctgt agtatgtgca gttataggga tatttgctac ttatgcttta aataaaataa
     2761 ataaaagaga aaataaaggt ttaaacaata aaattgct
//
LOCUS       DAENCV010000253         2729 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.256, whole genome shotgun sequence.
ACCESSION   DAENCV010000253 DAENCV010000000
VERSION     DAENCV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.256"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            218..1636
                     /locus_tag="KQD82_003824"
     CDS             218..1636
                     /locus_tag="KQD82_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG4382257.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1712..2524
                     /locus_tag="KQD82_003825"
     CDS             1712..2524
                     /locus_tag="KQD82_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4382258.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1015 a          277 c          483 g          954 t
ORIGIN      
        1 ttaataaaaa aaaattcaaa ttataaatat taaatgaata taattaaaac aaatatataa
       61 atttaagtat aacactcaca ttttaatgta ttgtgaaatt tttgttgaac tttatgtacc
      121 ataacttata aaatttatgt catgttatat ttgaataagt aataaaattg tagatgtaaa
      181 ttaatctaca aaatttatat gaaatggagt tgatgctatg acagaattaa tgattataag
      241 tgggttagta ctattgattt gtataacttc tagcaaggta ttgtataaat ttggagtacc
      301 aatattattg atttttatct tactagggat gttatttggg tctgatggga tggtaggaat
      361 ctattttgat aactatgaac tgacaaaaca attatgttct attggactaa tatttataat
      421 gttttttgga gggtttggaa caaactggaa tatggctaaa cctgttgcta taccttctat
      481 attaatgtct tcactgggtg taataattac ggctggcgtt acaggtatgt tttgccacat
      541 ggtaatagga actagtttac ttgaaggttt attaattggg gcaattgcag cttcaacaga
      601 tgctgcatct gtgtttgctg tgttgcgttc tcaaaaacta aacttaaagg gttcacttgc
      661 atcattatta gaagttgaaa gtggaagtaa tgacccaata gcgtatatgt tgacactaat
      721 tcttttgaca ttgatgaata attctggggt agaacttatt gcaccaatgg taataaaaca
      781 aatagctttt gggttagcta taggatttat actagcaaaa ttatctgttt atattttaag
      841 acattcaaac ttcgagatag aaggatttta tactatattt ataacagcta ttgcaatact
      901 ttcttatgca tttagcgagt atcttggagg aaatggatat ttaagtgttt acatttcagg
      961 tattattata ggaaatagta ggatacctct taaaaaaagt ttggttcatt tctttgatgg
     1021 aatttcatgg attatgcaaa taatgttatt ctttatgtta ggattgttat ctttcccttc
     1081 taagttacca aatgtatttg gtatagcaat ggccatatct atattcatga tagttattgc
     1141 aagaccatta gcaacattta tagttctagc aaaatttaaa ttctcaaata aagaaaaatt
     1201 attcatatcg tgggtaggtc ttagaggtgc ggcttcagta gtttttgcaa tttatgctgt
     1261 tactcaagga gtagccatag agaatgacat attccatata atatttttta tagcgttgct
     1321 ttcagtagga atacaaggtt cattgatacc aactgtagcc agaaagttgg atttagttga
     1381 tgataataca cctgtattaa aaacatttaa tgattatgat ggagaaatta gctctaaact
     1441 tatcgaagta aatgttgatg agggtaataa atgggctaat aagagtataa tagattctaa
     1501 tataccagac gatatattaa tagttatgat aaaaagaaag gaacaggttt taatacctaa
     1561 aggttcaacc attataaaaa caggggatat tttggtattg agtggagata gaattgaaga
     1621 attaatacaa ttataattta ttttgtattg actttaacct aaggggaaag tttaaactta
     1681 aattaatcaa atctaatgcg caggtgatga gatgttttct ataggaatgt tttctaaaat
     1741 aaataaaata actacaaaga cattaaggta ttatgaagat atagggttgt taaaaccaga
     1801 atatgtagat gaatttacaa aatataggta ttatacaaca gagcaattac ctaaattaca
     1861 tcagataata actttaaaac aaatgggttt atcattagct gagataaaaa aagctgtaga
     1921 aaatcctgaa gaagttgaaa atattttaat gaataaagaa aaagaaatgt tggatgtaat
     1981 taaaagtgaa gaatataaaa taattaaact acgtaattac cttatgaatt tgaaaggaga
     2041 agtcaatatg aatgatgtaa ttattaagtc tttaccaaaa gttaaagttg cttctatgag
     2101 aaaagtgtta aaggggtatg atgaactttt taatctattt ccaaatgtaa tggctgaaga
     2161 aatgatgaaa acagggtgta catgtgcagt tcctgattat tgttttaata tatatcatga
     2221 tggtgaatat agagagactg atatggatgt tgaaatgtgt caagctataa atgatttaaa
     2281 ggaagatacg gatatattaa aatttaaaga attagatgca gttgaaaatg cagtttgtat
     2341 attacacaat ggtccatatt caacattagg aaaaacttac gcagatgcat ttaaatggat
     2401 agaggacaat aaatataaag tggttggaaa tcctagagaa tcttatatag atggtatatg
     2461 gaataaagag agtgaagaag actggttgac agaagttcag ataccagtga aacgcatgtt
     2521 ataatatgtc tacaaagtat aaataagaat aaaaaaggtg gtgtctcaaa gtaaacattt
     2581 tagtttatga gacactcttt ttcatatgaa atcaagtata tttttataat tacgattatg
     2641 aaaaaaggct tatctatatt tttagacagc ctcttatttt tttattataa tagttttcta
     2701 aagatgctct atttataaat ttttattaa
//
LOCUS       DAENCV010000254         2725 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.257, whole genome shotgun sequence.
ACCESSION   DAENCV010000254 DAENCV010000000
VERSION     DAENCV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.257"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            299..955
                     /gene="nth"
                     /locus_tag="KQD82_003826"
     CDS             299..955
                     /gene="nth"
                     /locus_tag="KQD82_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG4382259.1"
                     /translation="MKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILS
                     AQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLC
                     ELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPN
                     PQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK
                     "
     gene            1149..1619
                     /gene="trmL"
                     /locus_tag="KQD82_003827"
     CDS             1149..1619
                     /gene="trmL"
                     /locus_tag="KQD82_003827"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG4382260.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1655..2503
                     /locus_tag="KQD82_003828"
     CDS             1655..2503
                     /locus_tag="KQD82_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG4382261.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         1128 a          262 c          521 g          814 t
ORIGIN      
        1 aatggataag aaagatataa ttgagaagga agagataaat aaaaaaggaa tgaataagaa
       61 agatataatt gagaaggaag agataaatga aaaaggaata gataaaatga ttggtaaaaa
      121 agatgagata attgatgaga aagatataga gaattttatg gatattgaag aagataaaaa
      181 aagtatcaaa gtaagtaaaa aaagtactaa aaaaggtgaa acggagaaaa aaacaaagaa
      241 gagtagttct aataaggcta cacaaaaaac aagtaaagaa actaaaagta agtctaaaat
      301 taaaaatgag tcagcaaatg aaaaagacgt aaataagatt ttagacgagt tagaaaaatt
      361 gtatccagat gctaaatgtg agttaaatta tgggacagcc tttgaacttt taatagcaac
      421 tattttatca gcacaatgta ctgatgtaag agtgaataaa gttacttcag agttgtttaa
      481 aaagtacaac acagctagag actttgctaa tttgagcatt gaagaaatta gtaaagagat
      541 aaagagttgt ggtctttaca aaagcaagtc tcaaaaaata aaggatacat ctgaacagct
      601 atgtgaatta tatgatggtg aagtacctga tagcttagaa aagcttataa aattacctgg
      661 tgttggaaga aaaacagcag gggttgtact tagtaacgct tttaatcatc cagcaatagc
      721 tgtagatact catgtattta gagttagcaa tagaatagga atagtggacg agccaaatcc
      781 tcaaaaaaca gaatttgcac ttatggaagc tataccaaaa gaaagatggt cacactcaca
      841 tcatgtatta atttttcatg gaagaagaat gtgtaaagct agaaatccag aatgtgcaag
      901 ttgcccaata aaagaagatt gtaattatta taaagaactt aatgaaacga aataactttt
      961 agaaagtaag ttgtacaaga gggattttct gatataataa tacttgtgaa aatgttagat
     1021 aaaaaactat tatatacatg aaataaaaat tattattaac attattttat ataatagtat
     1081 aatattattg ttattctgtt tataaaataa aatttgataa ataaaagcga tacttaggga
     1141 ggattaaaat gtcattaaat atagtattag tagaaccaga aatacctcaa aatacaggaa
     1201 atataataag aacctgtgca gcaacaggtt caactttaca tttagtaaaa ccactgggtt
     1261 tttcattgga tgataaacac ctaaaaagat gtgggcttga ctattgggat atagctgata
     1321 tacaatatta cgatagcttt gaagaattac aagaaaagta tccaaatagt aaatactttt
     1381 tttctacaac aaaggctaaa cagtcacatt cagatgtgaa atatgaagaa aattgtttta
     1441 tagtatttgg gaaagaaaca aagggattac cagaaccttt gttgaaagca aatatggata
     1501 cttgtattag agtgcctatg ttggatgttg aaaaggctag gtcattaaat ttatcaaatt
     1561 cagttgctat tgtagtgtac gaggctttaa gacaaatagg atacccaaat atgaggtagt
     1621 ttcaacaagc ctaaatgaga aaggagcata cttaatgaaa aatctaaaga taatctgtga
     1681 cagtttatcg gacgtgccaa aagatttgtt agataaatat gatatagata tagtttcatt
     1741 aacagttata ctggaggaaa gagaatacaa ggatagaatt gatatatctg gagatgaatt
     1801 ttataaaagg cttagagaag aaaatgtata tccaaaaact tctcaagcta cttatgccca
     1861 atttaaggaa atttttgaga aatatacaaa tgaggataga gagatacttt atatagcagg
     1921 gtctgccaca gctacaggta cgtatcaaag tgctgtaatg gcaaagaatg atgtagatgg
     1981 taaaatatat acatatgact ctaacttgct ttgtatggga actggtttac ttgtaataaa
     2041 agcaggagaa cttgcaagtg aaggaaagtc tgttgctgaa atattgcctg ttttggatga
     2101 attaaaagag aaggcttttg ttatattttc agtagataca ttggaatatt tacaaaaggg
     2161 aggtagaata tcatctacta aggcagcaat aggaagtata ttaaatataa aaccgattct
     2221 tgaaataaga gatggattag tttcgcaaat atctcaagta agaggaaaga agaatgtaat
     2281 ttctaagatg atggaattga taaaatcaaa ttgtggtgat gatttatcag accaaattgt
     2341 gtatatagga tatagtgatg attttaagga aaaggaacaa ttatctaagg ttatgcaaga
     2401 tgaattaaga cctaaagaaa ttagattttt ccaaattggt gtgtgtatag gtgcacatgc
     2461 tggtcctggt gttactggta tcatatgctt aaaaaataaa taatatattt ttgggtgaca
     2521 ttatttattt ttttattaaa ttaggataga atattaatta ttgagatgtt aaattggatt
     2581 tattttgtat gaaattaagt gttgatatat tagatttagt ttgtagggaa gtgtgtaaat
     2641 agataagaat ctattgcaca cttttttata gtaagattta ttaaaaaaat ttcaaaatgt
     2701 acttgtaaat tgtatataaa aaaat
//
LOCUS       DAENCV010000255         2701 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.258, whole genome shotgun sequence.
ACCESSION   DAENCV010000255 DAENCV010000000
VERSION     DAENCV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.258"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            298..2433
                     /gene="rnr"
                     /locus_tag="KQD82_003829"
     CDS             298..2433
                     /gene="rnr"
                     /locus_tag="KQD82_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG4382262.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQIGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1173 a          263 c          496 g          769 t
ORIGIN      
        1 accaatccta tttgatggtg tataattagg tgataaagag taatttaagc aattatgata
       61 aagaataact taagtaatta taatgatatt tacaaataat ttataaaaca tcataatttg
      121 tttgaagtta gtatgacaaa gcttacatta aatcaaaaat ataaataagt aattttaact
      181 tgtaataata ataaggaaat atagaaaatt atgtgataag taaaaaatgt tttgtctgaa
      241 gaaatataaa agacaatata aataataata taatgaaaag taaaacagga gggaaaaatg
      301 ataccaggac ttaaagaaag tttgctagga ttaataaatg aaagtgcata taatcctcta
      361 aaaaaagaag aattagcaga aatatttgat atacattcat cagaaatgcc aatgttttat
      421 aattttttaa atgaattaga agaagatggt tatatctgct ttactaaaaa aggaaaaata
      481 gtatctccca atcaaattgg atactttgtt ggaaaatttg tatcacacaa aaagggtttt
      541 ggatttgtag aatctgatgt tgaatataca caggatttat ttatctcaag tgacaatata
      601 aatggtgcta tgcataatga tagagttatg gcagaaatag tagtacctgc aactgaagaa
      661 aagagagcag aaggaagaat agtaaaagta ataaagagag aaataactag aatagtaggt
      721 accttccaat caagtaaaac ttttggattt gtaacaccag acaacaagaa atttactaaa
      781 gatatatata tacctaaaaa acattttagt ggagcagtag ataatgataa ggtagtatgt
      841 gaaattacag tatggccaca agaggataga aaaccagaag gcaaaattat agagatacta
      901 ggtcaaaaag gtgaaagagg cgtagaaata gattctataa taagagaaca tggtcttcca
      961 gaagaatttc caaagaaagt attgcaagaa gcagaagctg tagctgttga gattcctcaa
     1021 gaagagataa aaagaagaag agatttaaga gatttaaaca tatttactat agatggagat
     1081 gacgcaaaag acttagatga tgccatatca atagaagtat tacctaatgg aaacttcaaa
     1141 ctaggagttc acatagctga tgttacacat tatgtaagag agaaaaataa acttgacaag
     1201 gaagccttaa aaagagctac atcagtttat ttagtagata aagtaattcc aatgttgcca
     1261 aaaactcttt caaatggagt atgtagctta aatccatttg aagataagtt aactctttct
     1321 atatttatgg aaatagacca taaaggaaat gtggttaaac atgaaatatg tgaatctgta
     1381 ataaattcaa aagctagaat gacttataca gaagtatctg atatattaga aaaggatgat
     1441 gaaaaactta aaaagacttt tgaacatgtt gttgaagatt ttaaaaatgc tgaaatttta
     1501 gctaggattc ttatgtcaag aagggaaaaa agaggagcta tagacttcaa tttcccagag
     1561 gcaaagataa ttctaaatgg aaaaggcgaa gttgtagata taaagccata tgaaagaaga
     1621 atatctaata agataataga agaatttatg cttataagta atgagacaat tgccgaacat
     1681 ttttactgga tgggaatacc gtttgtgtat agaatacatg agacaccatc tttggaaaaa
     1741 atggaagaat tgagtaagtt tatatctaca tttggatata taataaaagg tgataaagag
     1801 gaagtacatc caaaagcatt acaaggtata atagagaaaa taaagggcaa aaaagaagaa
     1861 ggagctatca gtactataat gcttcgttct ttaaaacaag ctaagtactc accagaatgt
     1921 agtggacatt ttggattagc agctaaattc tactgtcact ttacttcacc aataagaaga
     1981 tatcctgacc ttcaaattca tagaataata aaagaatctt taaataataa aataagtgga
     2041 aaaagacaag aacagcttac tactatagtg gattatgcat ctgttcaatc atccgagaaa
     2101 gaaagaaagg cagagttagc tgaaagagat gtacatgatt tctataaggc tctttatatg
     2161 aaggataaag ttggtcaaga atttgagggt gttgtatcta gcgtaacttc atttggaata
     2221 tttgttgagt tagataatac tatagaaggg ttgataagac ttgcaaatat gaatgatgat
     2281 tactatattt atgatgagag ttcttatagt gttttaggag aaagaactaa gaagtcatat
     2341 aagataggag accttgttaa gataaaggtt gaaagtgtta atgtagattt taaagaaatt
     2401 gactttgaga tagttgaaaa aatagaggag tagataaaac agctaaaata agagataaca
     2461 agttgtagta tttatgatat gtataaatat atagtgcaca atactctata aaataaatga
     2521 taaaaatagg taattgagaa atatctaatg gaaatagtta actgaaagat aaataattaa
     2581 gaaatattta atgaaagtag ctaattgaaa aataagtaat tgagaaatgt ttaatagaaa
     2641 gagctaactg aaagataagt aattaagaaa catttaatag aaaaagccaa ctgaaaaata
     2701 a
//
LOCUS       DAENCV010000256         2463 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.260, whole genome shotgun sequence.
ACCESSION   DAENCV010000256 DAENCV010000000
VERSION     DAENCV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.260"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            83..682
                     /locus_tag="KQD82_003830"
     CDS             83..682
                     /locus_tag="KQD82_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382263.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(852..1319)
                     /locus_tag="KQD82_003831"
     CDS             complement(852..1319)
                     /locus_tag="KQD82_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382264.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            1430..2374
                     /locus_tag="KQD82_003832"
     CDS             1430..2374
                     /locus_tag="KQD82_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG4382265.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
BASE COUNT          951 a          270 c          389 g          853 t
ORIGIN      
        1 tataaaaata tagaaatact aaattaagaa agaaaattat taaaattttt tatagtataa
       61 aacatctagg agggtaataa aaatgaaatt aaaaagaact ttatttgtag gattagcact
      121 agtattagct ataggtataa ctgcatgtac taaaaataat gaaaaaccaa gtgaaaatac
      181 aggaaaaaat cctaatgaat ctgtaaataa taatgaagga tatatgaatt attattcaac
      241 aagctataat gattatatag caggtctaaa tagatattct atttatgata ctccagaaag
      301 tataaataat cagtttaaag ataaagaata tccaggaaat gaaaaatatc taagtgatgt
      361 aaaggctgca tataaagata gtagagataa aatacaatca tttgtaacat ctcttaaaaa
      421 agacggtaaa acagaagata cagagttaaa gaaaatgaat gatgacctaa tcgctgaagg
      481 ggaaagatta gtaaaagata tagataagag aatagaaaaa ttagataagg tatctgatga
      541 agatatgaaa aaaggtcaaa atgactttat aaaattagtt catggaactg aagatgttgg
      601 aaatgatata ggtagtggtt ttagaaaaat gataaaagat atgaatgata gattaggaat
      661 aacaggaaat aataagaaat aatagaataa atagttttga aatttgaaat aaattatttg
      721 agatatagcg aagccttata aataatacat tttttggctt cgcttttttg catgttatta
      781 agatattaat ttttatcttg taaccttaat atttttatta tgatttattt agggattatt
      841 ttcatatgtt tttacttgtt ttttctcttt ttaaattttt gttttgcaaa attgtaagat
      901 tctcctatta aagttttaaa gttctcaaat gtcttttcag tagggcttag gacgcatatc
      961 catcccatcc atgagtagat aggatgtggc atgattgtgt ccaattttgt aaaatcaaaa
     1021 tccatatcaa ctatttgacc tacacctgga cgctttggaa tatatccaaa catttcaata
     1081 aaagtttctt tttttaatcc tatattcaca cggtatacat ttggacgact aactaacgaa
     1141 cctttatcat gattactgtc tttttcttta atggtaagaa catatactcc tcgttttaaa
     1201 ataccattag gattgtaata aattgctcgt tccccccaac tattcattaa taaaacatca
     1261 tctaagtttg atagacaata attgataata tcatcagcct taatagtatt attttccata
     1321 aagtatcagt cccttctttc tttactatta ttattgattt aaatgtaaga atatcttatc
     1381 atataatagt gacaattatt gtcactatat ttaaattagg agtataaata tgtcaagagc
     1441 tgagcgttta attgaactga tgataacaat aaatgcaaaa agaagtttta cagcaggaga
     1501 attagcagaa gaattttctg tatcaaaacg tactatacta agagatttac aggtgctgga
     1561 gagtattgga tttccattat attcaaaagt tggagcggct ggtggatatc atgtacttaa
     1621 agagcgtata cttccaccga ttgcattttc agaaagtgag gtaaaatcta tattctttgc
     1681 atatcagtca ctagaatatt ataatgattt gccatttgag caagaaacta tttctgtttt
     1741 aaaaaaattt ttaaattgta taccaaatga tattcaatat aatattgaga atataaggcg
     1801 taaatttgtt ttttggacac cagataggca ctgttcaaca ccattattga aagaattgtt
     1861 taatatagta atgaatgaat ctacaattaa aattgaatat tcatcaaagc aaaaaaatag
     1921 tgtacgtacc attgttccta ttggtttgta tgccatgaat ggactttggt attgtccagc
     1981 ttactgtata aaatcagaat ctattaagga atttcgtgta gacagaattg tgaagatttt
     2041 gagtatagaa aacttatcaa gtaagaaata taaggtttta tcttctatac atgattatct
     2101 taagaatatg gaagtaggta cagattatca tattaaaatt aatttgacag atgagggagt
     2161 aaaacgttgt gaaacagaat ttttgttggc aagagggttg aaaatcttgt ctaaaggtgg
     2221 atacatagat atgtatatac caaaatctac acttaactgg gtggctgaat attttttaac
     2281 ttttgggaaa aatgctacaa taatagaacc tatagaactt aaacatttga taaagtctaa
     2341 agtactagag ctatataatc atcattgtat ttaaattatt ttatttaata gtaggataga
     2401 gtttggataa aatgttaatt ttcttaattt actttacaga tgatttagat agattgatgt
     2461 tat
//
LOCUS       DAENCV010000257         2417 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.261, whole genome shotgun sequence.
ACCESSION   DAENCV010000257 DAENCV010000000
VERSION     DAENCV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.261"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(798..1835)
                     /locus_tag="KQD82_003833"
     CDS             complement(798..1835)
                     /locus_tag="KQD82_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG4382266.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNSTKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVFEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(1932..>2417)
                     /locus_tag="KQD82_003834"
     CDS             complement(1932..>2417)
                     /locus_tag="KQD82_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4382267.1"
                     /translation="LGTCPSMTVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGIS
                     LDLKKYLDIKVQYLSGGQRQSLSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDK
                     IVREKNITTLMVTHNLKHALQYGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYA
                     V"
BASE COUNT          799 a          358 c          308 g          952 t
ORIGIN      
        1 cctttgaaaa tatttttata attttaatta tttgctaatt ttgtgtataa aaaaaccaca
       61 tagtgtacaa aactatgtgg taaaaacacg aataagccac atagtttaat ctatgtggct
      121 cttatatagt taaatgaaaa ctttataaac aatatagcca acatagatac aatcccaaaa
      181 ttttagtagt cttaaggggt ctgtatctat gttaaaacgt aggtacaaac cctaaaaata
      241 gtggctataa taataataat attttctatt gtttaataaa gtattcataa ttatctctcc
      301 cttttaatat tttaattagt cagaaaatta tatgtattct cgacttgtta taaattataa
      361 attatacaat aataaaagtc aactgctaaa tttaattaaa atactaaata atgtatactc
      421 agaatacttt taaattaatg tattgtttgc aaaaaaacgt tatcctaagt tataagtgaa
      481 tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt atatcaatca
      541 aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag ctcaagtagt
      601 gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt ttatggaaaa
      661 tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc aaagggttag
      721 ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata ttacctattt
      781 tttgaaatta ataggtttta ttttgcagat tttgcatttt catctgactt tatatacttt
      841 atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc gttaattatt
      901 atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc ttctaatact
      961 tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc acaacaagct
     1021 aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt gttttcatta
     1081 gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg gacatataaa
     1141 acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga agagctgatt
     1201 cctttttcaa atacttcata attatttttc ttagcataat catgtagaga atcaacttgg
     1261 atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc ttttggagtc
     1321 aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt accagaaaca
     1381 tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc tgtaacagca
     1441 gtcattatta tcggaatatc tttagtagaa ttataagctg cttgtgcaga tggagtagat
     1501 atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact ttgtgtagta
     1561 ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc tttatagcct
     1621 ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagatgg atgttccact
     1681 aattgactaa tacctatatt tttttgtttt ttgctatctg tttctttatt ttcatttgaa
     1741 gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag tactcctgct
     1801 ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct taaaatttat
     1861 taattatatt tgttcatata ttcatatgat agattataaa tatttctgat taaaactaag
     1921 attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac tgttaaatct
     1981 tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat tatcaaccta
     2041 tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt tatatttttt
     2101 tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg gtcaagtgct
     2161 gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat aattagtgat
     2221 agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata tttttttaaa
     2281 tccaaagaaa ttccttcaag taagtgttcc aagtcattcg ttttatgacg aagacatttt
     2341 tttaagttca ataatttccc tttattaagt gctagagata aattttctct aaccgtcata
     2401 gaaggacaag ttccaag
//
LOCUS       DAENCV010000258         2411 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.262, whole genome shotgun sequence.
ACCESSION   DAENCV010000258 DAENCV010000000
VERSION     DAENCV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.262"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            74..1588
                     /gene="glpK"
                     /locus_tag="KQD82_003835"
     CDS             74..1588
                     /gene="glpK"
                     /locus_tag="KQD82_003835"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG4382268.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1702..2331)
                     /locus_tag="KQD82_003836"
     CDS             complement(1702..2331)
                     /locus_tag="KQD82_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG4382269.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
BASE COUNT          896 a          280 c          456 g          779 t
ORIGIN      
        1 atttattgaa atatgtgata ttaaattttt ctttttaata aattaaatat tatatgataa
       61 ggagaatatg gtgatggaaa agaagtatgt aatggcatta gaccaaggaa caaccagttc
      121 aagagctata ctatttaata aaaaaggcga aatagtgaaa attgcacaaa aagagttcaa
      181 tcaaatttat ccaaaagctg gatgggtaga gcatgatcct atggaaatat gggggtcaca
      241 aagtggggta atgagagaag ttatagaaac agcaggtata agaccagaag aaattgcttc
      301 aatagggata actaatcaaa gagagactac ggttgtttgg agtaggtata ctggtaaacc
      361 tatttataac gcaatagtat ggcagtgtag gagaacgtca gaaatatgtg atgaacttaa
      421 aaataaagga ttggaagaat caataaaaga aaaaacaggt cttttaatag atgcatactt
      481 ttcagcaaca aaagtaaaat ggatattaga taatgtagaa ggagctaggg aaaaagcaga
      541 acaaggagag ttattatttg gaactataga tacatggtta atatggaatt taactcgtgg
      601 aaaagtacat gtaacagatt actcaaatgc atctagaaca atgatgtaca atataaatga
      661 tttagaatgg gatgaggata tattacgaga attggacata ccaataagca tgttgccatt
      721 agtaaaacct tctagctatg tttatggtca tacagatgag cgcatgttat caggagctaa
      781 aatacctatt gcgggatgtg ctggagacca acaagcagca ttatttggtc aaaattgtgt
      841 tgaagaaggt actgcaaaaa atacttatgg aacgggttgt ttcttgctta tgaatacagg
      901 cagtaacata gttaaatcta agcatgggtt acttacaaca attgcttggg gagttgatgg
      961 aaaagtaaca tatgcattag aaggaagtat atttattgga ggagcatctg ttcaatggct
     1021 tagagatgaa ttaaagataa ttgaatctgc aaaagatagt gaaatgtatg caaatagggt
     1081 agaggatact aatggtgtat atgtagtgcc agcatttaca ggtcttggag ctccatattg
     1141 ggatatgtat gctaggggtt caattttagg acttacaaga ggagcaaaaa aagaacatat
     1201 agtaagagcc acattggaat caattgctta tcagacaaag gatgttttag aagcaatgca
     1261 aaacgattca aaattaaaac taaaatcatt aaaagttgat ggtggagcaa gtaataataa
     1321 ttttttaatg caatttcaat cagatatatt aaatgtagat atagatagac ccaaaatagt
     1381 agaaactaca gctttaggag ctgcttattt agcagggttg tctgttggat tctatattgg
     1441 aagaaatgaa attacttcaa agtggtcagt agaaaaagag tttaatccaa atatgagtga
     1501 agagaagaga tgtaaattat ataaaggatg gaaaaaagct gtatcgagag ctttaagctg
     1561 ggaaaaagaa gatgaattgg atatttaaaa attaatcaaa gttaaattta tttgaattta
     1621 aaaagagctt tatgtaaaat atgaagctct ttttttgttg ttaatcttgc atttttatat
     1681 aatcacacta aattatataa tttatttttc gttttcatat aaaaacattg gtatagaatt
     1741 gtaaatgtgt tggtcaccaa ctactaagag aatgtctcct tctaaaaatt caatatatgg
     1801 tccaggagat attataagtt cttcacctct tttaattgca actattgtag cacctgtatt
     1861 ttgccaaaat ttagtttgtg cagctgtttg accaatatat ttgcaattag aatttatggt
     1921 gaactgaacg ggcactaaag gattgattat ttcaagtctg ttagaatagt ctaaaatttt
     1981 atttatagta tctaatattt cattttctaa attagattta gtatctatta aattataaag
     2041 tgttgattta tatgatgata tatttgtaat atctctatat ttttttataa aagaagaagc
     2101 tttgtctgga gatagtacaa taacaccttt accctttatg cttttaacaa cgccaatgtc
     2161 ctctaaaatt ttgacagctc ttcttatcgt ttctggagat acattgtatt taccagccaa
     2221 aacagagcgt ccaaatagta ctgaatcctc ttgtatttca cccgtgtata ttttatttgc
     2281 aatatccaac gctatttttt ggtatattgg catcgtatag tttttatcca ttgtttttcc
     2341 cccaatacaa aatttatgtt aaaaaaatat ataatcttaa caatactatt gtataattta
     2401 taggtttata t
//
LOCUS       DAENCV010000259         2396 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.263, whole genome shotgun sequence.
ACCESSION   DAENCV010000259 DAENCV010000000
VERSION     DAENCV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.263"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            263..2335
                     /locus_tag="KQD82_003837"
     CDS             263..2335
                     /locus_tag="KQD82_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG4382270.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT          883 a          256 c          457 g          800 t
ORIGIN      
        1 tatgttcaat tttaatatat tatttaaaat ttaatataaa ataataattt taaaacatta
       61 agattgttac tgataatgca ggcaagactt atgtttataa aaaaattata atagtttatt
      121 tttaattctg aaattaggtt aatataggtt tgtttatatt aacataagtt taattttaga
      181 attaagtata ctaatgtaac tttttataaa cttaagtctt ttttatttaa aaaaatataa
      241 aataataagg agagaaaagt aaatgaaaaa agtatttggt gttttacaaa aagtaggaaa
      301 atctctaatg ctaccagttg cattacttcc tgcagcaggt atattacttg gcgtgagtaa
      361 tgctttagca ggagacgcat tgttggctca ttttccagct ttggcgaatc ctacatttca
      421 acttataata agtataatgg aaagttcagg tcaaattata tttaataact taccacttat
      481 atttgcagtt ggggtagctg ttggtttaac tgaaggagaa ggtgttgcag cattggcagc
      541 catagtaggt tttttaattc tcaatacatc tatgggtgtt atggcaggtg ttacagaaga
      601 tatggtagac aattctatgt atgcaaatat tgttggaata ccaacaattc aaacaggggt
      661 atttggtggt attattatag ggatagtggc tgcttatctt tataaaaagt tttataaaat
      721 tgaattacct cagtatctag gtttttttgc aggtaaaaga tttgtaccaa tcgttacttc
      781 attagttgct atagcaatag gaattatatt gtcttttatt tggcctccaa tacaagaggg
      841 attacttagt ttctcaaaaa atgttataga agcaaatcaa acactagcag catttatgga
      901 aggttctttg gaaagactat taataccatt tggtttgcat catgttttcc ataatccatt
      961 ttggtatcaa tttggagaat atgtaaataa agctggtgag cttgtaatgg gtgaccaaaa
     1021 gatattcttt gctcaactta aagatggagt aaatttcaca gcaggtactt ttatgacagg
     1081 tgcatttcca tttatgatgt ttggtcttcc agcagcagct cttgctatgg tgcatgaagc
     1141 aaagccagaa aagaaaaaat ttgtagcagg tataatggca tcagcagcat taacctcttt
     1201 tttaacagga ataaccgagc caatagagtt tgcgttttta tttgttgcac cagtattatt
     1261 tgcaatacat tgtttatttt caggtttatc atacgcaatt atgcaattat taggagttaa
     1321 aattggattc acattttcag gaggattaat agatttctta ttatttggtg ttcttcctaa
     1381 tagaactcca tggtacttgg ctatcttggt aggtattggt tttgcagtat tatactatag
     1441 tgtatttaga tttatgataa gaaaatttaa tttaaagaca ccaggaagag aagatgagtc
     1501 agaaactaac gctgaagtca ttgaaatgtc taagaatgaa ttagcatcag agattttgat
     1561 tgctttagga aataaagaca acttgaagag cctagatgcg tgtataacaa gacttagatt
     1621 ggttgtaaat gatataaata aagtagataa agaaaaattg aaatcacttg gagcagcagg
     1681 tgttatggtt attggcaaca atgttcaagc tatatttgga ccaaaatcgg attcaataaa
     1741 gtctgatata aaagatataa tagagggaaa aacaaaggtt gaaagtactg ttactaaaaa
     1801 tgttgataaa aataaagtga aaaaaaatat ggaagttgca aatatggaaa atgctttagt
     1861 agaaaagatt gattcattaa caaatggaga gatattagat atagaagaag tgccagattc
     1921 tgtattttca agtaaactga tgggggatgg atttgcaatt aaatctagtg atggattgat
     1981 ttattcacca gttgatggaa ctattggagt tatatttcca actaagcatg caataataat
     2041 aaaatcccaa aaaagtggag tagaaatact tatacattta gggatagaaa ctgtaaattt
     2101 agaaggaaat ggatttgaag tatttgtgaa tgtaggagat gaagtaaaag cgggagaaaa
     2161 attagttaaa atggacttag aatatattga aaaaaatggt ctatcaacaa taagtcctgt
     2221 agtatttacg aatttagaac aaaatcagaa attaagcatt aaaaaaggag taaccacagc
     2281 aaaagaggag aatagagtaa gtatattaag agatgatatt gaaaaaatag gatagctata
     2341 tgatagtgta actaaaagtt atgtgagatt taataaatat ataaataggt tcagtt
//
LOCUS       DAENCV010000260         2363 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.264, whole genome shotgun sequence.
ACCESSION   DAENCV010000260 DAENCV010000000
VERSION     DAENCV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.264"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            131..319
                     /locus_tag="KQD82_003838"
     CDS             131..319
                     /locus_tag="KQD82_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG4382271.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="KQD82_003839"
     CDS             complement(479..631)
                     /locus_tag="KQD82_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG4382272.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="KQD82_003840"
     CDS             complement(729..1205)
                     /locus_tag="KQD82_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG4382273.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="KQD82_003841"
     CDS             1424..2185
                     /locus_tag="KQD82_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG4382274.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
BASE COUNT          880 a          323 c          312 g          848 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac aactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccgc ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctatagaccc tctatttttt agtaaagata gtctataaat
     1261 tttatttttt aattttcatt agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatgta ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaaa ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaaca aaaaacttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttga gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcagat atatttttat cattttatca
     2281 atgaaaaaca ggtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa att
//
LOCUS       DAENCV010000261         2269 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.265, whole genome shotgun sequence.
ACCESSION   DAENCV010000261 DAENCV010000000
VERSION     DAENCV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.265"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(137..1429)
                     /gene="brnQ"
                     /locus_tag="KQD82_003842"
     CDS             complement(137..1429)
                     /gene="brnQ"
                     /locus_tag="KQD82_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG4382275.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNNNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          759 a          373 c          247 g          890 t
ORIGIN      
        1 aataaatttt taaatttatc aaataggatt aataatgttc ttttatctac aataatttaa
       61 taattctttt aaatataaaa aggaattgcc tatataattt aaaggcaatt ccataattat
      121 caattagttt ttattattat gcagttttac tgcttttaga tggaataaag tttcctatta
      181 ttcctgctat tacaactgga actatccagt taaatcctaa tgaagcaaat ggtaagctat
      241 taactagagg aatttttaca ccaaaacttg ttgcaactgt taatacactt actaataatg
      301 ccatgtatgc agcacatttg aacacgttgt tatttttaat tttattacta aataatgcta
      361 gtataactag agttatagtt gctggatata ccatacttag tataggagaa gagaattgta
      421 ttattgtact tactccaaag tttgatatga ttgcactaaa tacacaaact actataacta
      481 tatgctcata ctttaatttt ccatctgtaa gtgttgagaa atattgacca gctgctgaag
      541 aaagacctat agctgttgtt aaacatgcta atgcaactat aatagccaat aaaattttac
      601 ctgtagttcc aagtaaagct gctgttattg caactattaa agctgtttgt tgaacatctt
      661 gtccatattg ttgagacaca gttgctccaa gataacataa tccaccataa actaatgcca
      721 ttcctattac tgcaattaca cctgcactta ttgtggcttt catcttttct ttctcacctg
      781 tataaccttt agctattact gacatgatta taattgatgc aaatacacta gctgccaatg
      841 catccattgt ttgataacct tgagttatac cttctgcaaa tacattttgt attaaagaac
      901 tgtctcttat ttctcctaat ggagatacga tacctttaac tattaatact gccaaagcta
      961 taagtaaagc tggagttaat attgacccaa ctatatctac aacttttgat ggtcttattg
     1021 ttaaaaccaa tactagagca aagaatacta tagagaatat tactgggctt attccagaac
     1081 ctattgtagg taatataccc atttcataag ttgttgctgc tgttctaggt attgctaaaa
     1141 gtggccctat acagattacc attgcacatc ctaatgcaat acttaatcct ttgcctgctc
     1201 ttgagaacat cttcgatgtg tctccattga acttagttgc tgctataact gctaataatg
     1261 ccaaaccacc atctgcaaaa aggaatccta tgaaagctac tagccaacta cttccagata
     1321 aaactcctag atatggaggg aagattaagt tccctgcacc aaaaaacatt gcaaatagtg
     1381 caaatccaat tacaattaca tctttaaaat tgatttgttt ctcacccatt taaagcaccc
     1441 ccatttgaat tttactacct tttactaaaa aataagtcca atctcttaat taaatgaaaa
     1501 ccttttgacg agattttatt taattataca tttttgactt aaatttgtca attatgtttc
     1561 ataatttata aaatcttcac aatatttaat acaactttct taattaaaca aaatcaacct
     1621 gtatacttta aacatagttg gttttttgtt ttttctctaa tttttcttga taaaattaac
     1681 tattccgaat aattaaaata ttaagacttt ttagattatt cgatttatta ttaagttgta
     1741 ttgctttata tactgtatat atcactctcc ttttttacat tcatataatc tttttaattc
     1801 aatttaaatt ttaaatattt tgtgtttatt atataatctg aaaaaaaagc agtcaatagc
     1861 atttttaatt tttttaattt tcaaaattat gattcttaaa aaatcgtttt ccttttatca
     1921 taacacctat ttacgttaat tcttgtagta ttttgtattt attttgctaa attgacagtt
     1981 aactttattc aatttttatt ctacttgcat agatttgcct cttgtttttt actcttaaaa
     2041 ccattttttc tgaaaattcc ttattatgat ttatcaactt atttttcttc ataacaatta
     2101 ctctctaact tccaacttac ttaaactttg cagttgctag tattcacgtt tacatactta
     2161 tatgtcataa ttaactaaaa attcttaata tatctctcaa tgaaaacaat ttactcattt
     2221 atttaataca tttatataat catatgtctt aaattttgtt ttattattt
//
LOCUS       DAENCV010000262         2222 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.266, whole genome shotgun sequence.
ACCESSION   DAENCV010000262 DAENCV010000000
VERSION     DAENCV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.266"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(102..1669)
                     /locus_tag="KQD82_003843"
                     /pseudo
     CDS             complement(102..1669)
                     /locus_tag="KQD82_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(2005..2187)
                     /locus_tag="KQD82_003844"
     CDS             complement(2005..2187)
                     /locus_tag="KQD82_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382276.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          686 a          309 c          250 g          977 t
ORIGIN      
        1 agataatatt taaataaaaa ataccttata aagttaattt tttaatactt tataaggtaa
       61 atacatattg tattgtgggg ataaatttaa atcatttgat actaatcaac actaagaacc
      121 aataactctc tatcatcttg agtaattgta tatattctta atttcttgta tgtcataata
      181 ttttctccac ctgtaaaata actcctaaga ttaacacaat cagttttagg ttgatttgta
      241 tctcctaaat aattacgcaa ttctttatct ttagtttcaa tatctttaaa tccaaaatat
      301 tgtaaagtag cttcaactct atatttttgg acataatcga gagcttcttt attagtttat
      361 gttggtgtaa aatacatacc atttatataa gcctttattg tacttggatt aaatttaaaa
      421 tctgcagaat atattttatg tgtaactatt atttcttgaa cagttggaat tttaacttct
      481 ggttcatcaa gtatttcagg agtactattt agctctgtaa tagataagtt atctaagtat
      541 attgtatcac tatcaattaa tgctatattt atattagaag aatctttttc agtagtttca
      601 aattcataac taaattttgt atatccttgt tctagtacca aataatccgt tttttcttct
      661 tttactttta tatttacaat tattgaatta tatgttaaag gattttttga attttactaa
      721 aaacataacg tttataaggt tttagttcag acataggtat tttaatcttc gtctcaccat
      781 ttaatctatt tgctgagcct tttaaaccat cttgatttgt agtattaaca ttactccata
      841 tactagggta attattgtaa tcatcaaaat tagtaaagta agttggtgtt tttataagta
      901 tattcattcc tctttcaagt ttaacattac atatttgttt atcagacaaa gtttttaaac
      961 tgtctttaat cttattgcct atattatcat caaatatgag ttcaacacaa ctttcatcta
     1021 ttggtatatt attaaaatat aacaaaccat cttttttagt agtgccaaaa gttttttcaa
     1081 ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg gacctgtaaa cttttagcag
     1141 taattcttct tttgtaagat tcattttctg tatctaatat aatagataca gaaatactgt
     1201 caatttgacc tatatcgtct taccaactat ttcattctgt tactatttgt ccagaaccat
     1261 tttttgtacc aaaatttcca cttacttgtg ttgtttctaa tttaatttgc tttccatcat
     1321 ctaatttttt taattaatta taatttatag gaatcagtct agaactaaat tgatacattg
     1381 tgttaagagc cataggtgaa aatccttttt tgagataagt atttccagga gatagattat
     1441 tgccaatttg attttcttgt gctttgatag ttgataatgc atctccatct aacactaaat
     1501 ttgttgttgg tgtcactttg tacataggtg cagtacctgt gttgtagtat ctaatatttg
     1561 catttatata tgcagattct cctttgttta tacttaatcc agtatttcat gattatccat
     1621 tgctattttg aacagcagtt gaattatctg ttgtatggta ataatttgtc ttgtatagtt
     1681 tctttaaact cattaaattt ttctaatgaa atttcgtttt gattttttgt tcttattact
     1741 ttattaaatg caaatttttc tggagattca aaataatata catacatagg tttatctatt
     1801 ttattttttg atatagcatt tcgaagttct ctatattctt tttctcaaga atttgcttct
     1861 atttgataat cataaaaata atttcttgtc tgagaatatt tgcttatctc tatagaatct
     1921 tttttatatg atcctaatac ttctttttca gatttttcaa gtttttgatc ttttctttct
     1981 cattctttag ccttttcttt atctttaaaa aaatccttct gatctttcta taggagcctt
     2041 gtaagtagta ttacaaactt ttccactata gctttgtaaa tcttgtgcat aaatgttatt
     2101 aggtaacaaa ctacctaaag ttagatataa tatcaacaat atagatatac aattactaat
     2161 acttttatgt ttcctaaatt ttttcattta ttctccctac aaatattagt ttagttaact
     2221 tt
//
LOCUS       DAENCV010000263         2165 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.267, whole genome shotgun sequence.
ACCESSION   DAENCV010000263 DAENCV010000000
VERSION     DAENCV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.267"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            542..1870
                     /locus_tag="KQD82_003845"
     CDS             542..1870
                     /locus_tag="KQD82_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG4382277.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          784 a          234 c          360 g          787 t
ORIGIN      
        1 aaacatattt agattttaac atctaaaagg agttttcaaa gctaatattt catttttaaa
       61 taaaaaaata aaatattagt tttgatttct ggtttaaatt gggttataat aaattgtcaa
      121 attatttatt aatataaaat agactataaa acaagcaaaa atcatactat tatataaaag
      181 accttaaaga tagaaaacta cagaaagaag ccaaaatgtt tttgaaaaaa atgaaatttt
      241 attgaaaaaa aatcaaaatg aatttaactt aatagttata atctattttt agtgttacaa
      301 atatacaatg tatattatac aatataaaaa atacatttac ttagaagtat tattatattt
      361 ggatttttgg tttttataat ttttttatat ttaaacatag ctaaaaataa aagattacga
      421 aagctaagta gagtttattt tattttaata aataataaag ctatttttct atatattggc
      481 ataatatatg cttatttaac tatgaaatta aaattaaata ggattaatga ggaggaaaaa
      541 aatgggaagt aatgaactga aaaagactct tggcgtatca gcagcattat caactgttgt
      601 tggttccgtt ataggagcgg gcgtattttt taaaccacaa gcagtctata cattaactgg
      661 aggagctcca ggtcttggta tattggcatg gttaatagct ggaatcataa ctataacagc
      721 tggtcttaca gctgcagagg tatcagttgc gataccaaaa actggtggaa tgatggtata
      781 tataaaagaa atttatggag aaaaattagg ttttttaact ggatggatgc aaattgtatt
      841 attttatcca gggatgatgg cagcattagg tgttatattt ggagaacaag catcagcact
      901 aattggtagt ccatcacttt tacttccaat agcaattgga attatagtaa tagttgcagg
      961 gcttaatatg ttaggttcaa aaactggagg agtcatacaa actgtttcta caatttgtaa
     1021 gttaatacca cttattttaa taatggttgt tggatttatt aaaggtggtg gaaataatcc
     1081 aatacttaca cctatggttg gtgaaggatt aagcctgggt agtgtactag gtcaagtttt
     1141 aattgctata ttatttgctt ttgatggatg gatgaatgtt ggtactttag ctggagaaat
     1201 gaagaatcct ggaaaagacc ttccaaaagc tataataggt ggattatcag ttgttatggc
     1261 tgtttatttt ataataaatt tagcgtatct ttgggtatta ccagctaatg aattagcaaa
     1321 ttatgcttct cctgcatcag cagttgctga agttatattt ggttcaatgg gtggaaaaat
     1381 aatatctgta ggtatattaa tttcagtgtt tggagcatta aatggatttt tactaactgg
     1441 ttcaagggtg gcatatactt tagctacaga taagacttta ccaaagtatg gtatattttc
     1501 taaattaaat tctgctcaag ttccagcaaa tgcaatagca ttagtatctg ttattgcatc
     1561 tatatatgct ttgtcaggtc aatttaattt attaactgac ttagctgtat ttgctacttg
     1621 gatattctat gtattaactt ttataggggt tatgaaactt agaaagactc atccaaatat
     1681 accaagagag tataaagttc cattatatcc tattgttcct ataatagcta tagcaagtgg
     1741 tatatttgtt gtagttaatc aattgtgttt tgcaggtatg aaaactacta tgatatctat
     1801 aggaggtctt gtaataacag ctataggctt accagtgtat gcttatatga ctagaggagt
     1861 aaaaagataa accaagtagt ttacaaatgc ttcataatta aaataaaaga gactgtctca
     1921 aatagagata agtctctttt ttattgttaa aattgtaaag atatgtttaa ttttacaaaa
     1981 aaagagcgtt ccatgaacta aaaagtttat tttaaggtat tcattttatt tttagttttt
     2041 tgattataaa attaaacatc taaaaaatta tttattttaa aatactttat tttaaagtgt
     2101 tttttatcaa actaagtggt atctgtacaa caaatattat acatagcttt tatgaaaatt
     2161 aaatc
//
LOCUS       DAENCV010000264         2145 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.268, whole genome shotgun sequence.
ACCESSION   DAENCV010000264 DAENCV010000000
VERSION     DAENCV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.268"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(138..677)
                     /gene="scpB"
                     /locus_tag="KQD82_003846"
     CDS             complement(138..677)
                     /gene="scpB"
                     /locus_tag="KQD82_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG4382278.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENKLIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(679..1398)
                     /locus_tag="KQD82_003847"
     CDS             complement(679..1398)
                     /locus_tag="KQD82_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG4382279.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1428..2024)
                     /locus_tag="KQD82_003848"
     CDS             complement(1428..2024)
                     /locus_tag="KQD82_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG4382280.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
BASE COUNT          689 a          340 c          191 g          925 t
ORIGIN      
        1 ctatttatac ttaattatcc atatttattt taagagaatt ataattttat atttatacaa
       61 aaaaacacaa caacttagag cattttaaat cttttaaaat ttaaaattac atactaaatt
      121 attgtgattt tatattttta ttcatttgtt gccacttctt gataattttc tataggtggc
      181 aattcttcaa gactttctat attcaacaac tttaaaaatt catctgttgt tttataaatt
      241 attggtttcc ctatcttatt caatctacca gcttctctta taagtttatt ttcaaacaaa
      301 gtttgtaata ccttatcaca ttttactcct cttatatcct ctatttctac ctttgttatt
      361 ggttgtttgt aggcaattat agtaagagtt tctaatgtag cttggctcaa acttttcttt
      421 ttctttggtt caataatctt ctttatatat tctgcatagt ctttatttgt acacatttgg
      481 tacttattct ctaacttgat tatttgaata cctctatttt gttctctgta ctcctcaata
      541 agtaaattta acataatctc tatttctttt gatgaaagtt ctttatttat tatatagttt
      601 aattctttta tacttattgg ttcaccatat gcaaacataa cagattctat aatatacttt
      661 atatcttctc ttttcatttt aacactccga actttttctt attagaatat catcaaaaaa
      721 tatatcttgt actacgacta tttctttttc ttttataagc tctaatatag ataaaaaagt
      781 agctattatt tcatcatttt catatgattt tataatattt gtaaaagtaa cttcaatctt
      841 atcttttata atatctctta tatataatat tttttcttct acagatatta ttttcttcct
      901 tactatttta tctagtttct catcattagc attttctatc tgtgatgttt taactttaaa
      961 tatgtatgga agtatatttt ttattgcttc aattgatata tcttctaaat ccacattgtc
     1021 atcaataatt atctcttctt tatttctata aaatctctca ttaacataag ttatattttc
     1081 ttttatatct tgtgatgcta ccttaaattt tctatattct tctaactttt ccataagttc
     1141 tattcttggg tcttcttctt catctttttg cttataaagc agatatttag actttatctc
     1201 taaaagtttt gatgccatag ttataaattc actagttatc tctaaatcca ttttatctag
     1261 cattttcaaa taatttagat actgcttagt tatatctatt atagatatat cctttatatc
     1321 tattttatgc tttgttatta aatcataaag taaatctaat ggtccctcat agacttgtaa
     1381 ttgtatatta tacttcatat caatccctct ttaatctgtt tattatatta aacaaataaa
     1441 tatactattt ttagcaacac tgtatatatt ggtgatagta ttatttgata tataccagta
     1501 aataaagcaa ttaataaaat tatataactc atactttcaa atttatacaa atattcattc
     1561 catttatatg gtacaaatgt agatataact ccccatccat caagtggagg tatcggaagt
     1621 aagttaaaag ctgcaaatcc catactatat aaaagcacat atccagctat tgtatttata
     1681 gcgtacatat gcacatactt attgataatt acacacagta tagcagtcaa aacattacaa
     1741 aaaacgcctg caagtgaaac tatcaggttt cctatcttat agtttttaaa gttatttgga
     1801 tttactggta ctggctttgc ccaccctatc ttaaggataa acatagccaa aagacctatt
     1861 gggtcaacat gtcttacagg atttaatgtc attctaccat aagctttagc tgtatcatct
     1921 cccaacaagt gagctgaata agcatggcca aactcatgta ttgatattgc catagctata
     1981 ccaactaaac ttgctactat ttctcctata ttcatactta acattaatgt acatctccta
     2041 aattaaattt ttaatattat tataacaatt aaatagctta taatcaaaat tattttacat
     2101 tataataatt ttaacttcta ggcataaaat taaaatttgc ttttt
//
LOCUS       DAENCV010000265         1981 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.269, whole genome shotgun sequence.
ACCESSION   DAENCV010000265 DAENCV010000000
VERSION     DAENCV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.269"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            230..844
                     /locus_tag="KQD82_003849"
     CDS             230..844
                     /locus_tag="KQD82_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG4382281.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            862..1224
                     /locus_tag="KQD82_003850"
     CDS             862..1224
                     /locus_tag="KQD82_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382282.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1330..1827
                     /locus_tag="KQD82_003851"
     CDS             1330..1827
                     /locus_tag="KQD82_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG4382283.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          752 a          215 c          331 g          683 t
ORIGIN      
        1 atttatataa ctcaattaat tttttaaata caactttata atataaaact tataataaaa
       61 gtaaatttat atgttaaatg aggttgattt tttattattt tttatttatg ctagtactaa
      121 agacttgcaa acgttttttg acaagtcttt attttgtcta aattaaacaa acttttttac
      181 aaataaatac ataaaattta aactaatcta tttattggag gagataaaat tggaagaagt
      241 aattgcaaaa attattacta tagaaaatgg attcaaagaa attgaaaaca ttgctaaaaa
      301 aattgtaaaa aataatgact caaaaaagtg ttattatcta tcaatctact tatataggtc
      361 agaatattat caagtaagag aattggctgt gtttatactt ggttttatat cagtaactat
      421 ttctgaagct ttgttatttt taaaagataa tgtgagtaaa gatgaaaact ggagagtaca
      481 agaaatacta gctaaatcat ttgattatta ttgcagtgaa gtaggatatg aaaaggctct
      541 tcctgttata aaagaatggc ttaattctga tagtccaaac ataaaaaggg ctgtgacaga
      601 aggtcttcga atttggacta gtagagacta ttttaaatct aatcctgacg ttgctatatc
      661 actactttct agtttaaaag aagatgatag cgaatacttg agaaagtctg taggtaatgc
      721 tcttagagat ataagtaaaa aatatccaga tttagtaaaa aaagaattag attcatggga
      781 tatatcaaat aaaaaagttg agtttgtata taagcttgct tcaaaatgta tcttggcaaa
      841 gtaaaatgga ggtgaactta tttgaaactt cgtgatgagt atacttgccc attagaacta
      901 acacatgata tgataaaagg caaatggaaa tctattatat tatggagatt acgtatagga
      961 ccaacttcct tatcaaaact tgaaaaggaa atcaatggta ttaatcaaaa gatgttactt
     1021 gaacaactta aagaattaat tgaatttggt cttgtagata aaaagacata tgaaggttac
     1081 ccacttaaag ttgaatactc acttacacct attcgaggga aacaaatatt ggatgctttg
     1141 gctataatgc aagatatagg aattagcttt atgttggata atggtatgga agatattctt
     1201 aaggaaaaag gtgttttaaa gtaaatagat acccacaaaa aagtaagtaa tttaccatta
     1261 ttttcaactg gtatataatt aaattataga tatattctat taatttatta taataggagg
     1321 taaattatta tgaaagtaac tagtacaggt atagtaaatg gtgttataga agataaatac
     1381 ggtaaaagag gaactcagtt caatgaaggt ggtatgccta catattcact acctctaaag
     1441 tttgaagatg ctccaaagaa tacagtatcc tttgctgtat ttttagaaga taaagactct
     1501 gttcctgtat gtgggtttgc atggatacat tggttagctg taaatataac aaaagatgag
     1561 ttaaatgaaa atgaaagtgt aacagctact gattttgtac aagggactac tagttggcat
     1621 ggtaaactag gaggtcttga cagattagag gccagtgtat atggtggaat ggctcctcca
     1681 gacagacctc atgtctatga aatacatgta tttgcacttg atactatcct tgaccttgaa
     1741 aaaggattct atatgaatga atttttcaaa gctattgaag ggcatgtttt agatacattt
     1801 acactaaaag gaagttattc taattaatta ggttgactat agctaatcat taattaaatg
     1861 ctacatttaa ttgatgatta gcttaattgt tattagaata ttttctgcac tttatcttaa
     1921 tatatatttt agttgactgg tattttaatt tctattagat tattatcaat ttatataaat
     1981 t
//
LOCUS       DAENCV010000266         1949 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.270, whole genome shotgun sequence.
ACCESSION   DAENCV010000266 DAENCV010000000
VERSION     DAENCV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.270"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(39..1256)
                     /locus_tag="KQD82_003852"
     CDS             complement(39..1256)
                     /locus_tag="KQD82_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG4382284.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1447..1860)
                     /locus_tag="KQD82_003853"
     CDS             complement(1447..1860)
                     /locus_tag="KQD82_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4382285.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          668 a          322 c          219 g          740 t
ORIGIN      
        1 ttagattata tattttaata tactttctta tataagaatt attttaaatc aagattatct
       61 agtattttta aaaatatttt tacagcagta tccaagtcat ctacagaaac actttcatta
      121 ggattatgac ttacgccccc agcgcatctt ataaatacca taccaatatc agttatatta
      181 tccatttcct gagcatcatg gccagcacca ctataaatat aaaaaggatt aagatttaaa
      241 tctataaaac ttttttctat gatttttgtt attttattag aacatggtac aggaacattt
      301 tcaaaagcaa gttcacttgt ataattaagt tttctatttt cacatatatg tgatatttca
      361 ttaaatatct tttctacaga attatcaaga atttcttgtg aacaagaacg tacatcgagt
      421 gtaaattcag ctctttcagg taccacattt gatgaagatg gatagaaatt catctttcct
      481 acagtagcaa ccaaatcagc agtcgtttta gctacttttt cgatagctaa tacacattca
      541 caggcacaac atccagcatc aagtctcata ttcattggaa gtgtaccagc atgtccagac
      601 ttaccattta ctgatatctt aaaactttta aatcctgtaa tacttgatac aattccagca
      661 ggaagagatt cattttctaa aacaggaccc tgctcaatat gaaattctaa atatgcatct
      721 acatcttttc taggatgcaa atttgttaag ttttttgtat taaatccaaa ctttgttaca
      781 gcttcttcaa gtgtaattcc attttcatca acagatttaa gaagttcctc tttaaaagta
      841 ccagcaaaaa ctttacttcc cattaaagaa gtttcaaatc tagttccttc ctcttcagcg
      901 aaagcaatta gcttaatatt gaaaggataa gacctattat tgtgttttgc ttcttcaaga
      961 ccaactagag gcactattat acctaacata ccatcaaata tacctccaca ctttacacta
     1021 tcttgatgag atgcaactac taatgtggga gcatttggaa agcttgattt atatgtacct
     1081 ataacgtttc caatgtcatc aatttcaata tcaagattta aaccttccat ccatgaacta
     1141 attaaatcaa tagatttttt atgttcttga gtcaagataa gtcttgtaag accatttgtt
     1201 gttgaactta tttttcctaa tgtttgtaga taatccatac atttttttcc taacatattt
     1261 acctcctaaa aatataattt ttatttaagc taagattagc ttttatactt ataatttttg
     1321 aactttcaaa taattttgta tgtatagtaa aatactattt tagtattaat aatgatataa
     1381 aaatataaag tctgtagacg aagaacagaa gttttaagtt aaaaatatga aatatgattt
     1441 ttattattat agtatttttc tataataata tctctttcca gttagaggat aattttcaag
     1501 tgaaaatact ttatggtatc caaatttctc ataaaattta ggtgcttgaa aactgaatgt
     1561 atctaaaaaa acatatttac aacctcgttc tttagcaatt ttttcagcct tatataaaag
     1621 ctgacttcca gtacctttat atctaagctt ctcactaacc cataagtatt ttatagaaag
     1681 ccaattacca tgagtaacac ctataaaacc tgcaacaatt ttacctgctt tatctcgaat
     1741 atatattcct aaatccactg ggtttttatc ttcaagtcta gcaagattat attttaataa
     1801 tccttgaaaa atagtatctt catcttgttt ttctattttt tctgtaattt ctaattccat
     1861 atttgaactt tctccttgta taatcttttg tatattggta ttttttaata aaatcagtag
     1921 aaagaatata gtttaacatt attctaata
//
LOCUS       DAENCV010000267         1926 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.271, whole genome shotgun sequence.
ACCESSION   DAENCV010000267 DAENCV010000000
VERSION     DAENCV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.271"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            178..462
                     /locus_tag="KQD82_003854"
     CDS             178..462
                     /locus_tag="KQD82_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382286.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1233..1448)
                     /locus_tag="KQD82_003855"
     CDS             complement(1233..1448)
                     /locus_tag="KQD82_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382287.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1459..1926)
                     /locus_tag="KQD82_003856"
     CDS             complement(1459..1926)
                     /locus_tag="KQD82_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG4382288.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFFDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          786 a          204 c          236 g          700 t
ORIGIN      
        1 atgaaaatat acttatattt tactaatata catttaaaaa ttataattta aagcattaaa
       61 aaataaattt gtattttgtt ttatataaag taatttgaag aatatggaaa aacttgatta
      121 gtacattatt aattagggta taaaatggtg taaatagaat gataaggggt ggatgaaatg
      181 cttgtatata ataaaagttt ttatcctaat gacatatttc caagattaga tttttcaaaa
      241 ataaaaaaac agttaaaatt gatagataat gacctgtcag attttggaag catatgtata
      301 atagaaaaag aacattatac aataagtgta aacagtatag gtgaaataaa tgtgtattat
      361 gatttagagt acgaaaataa ggtgtataga atagtttatg agattgaaaa gttatttaaa
      421 tctcaagttg gaaggtttag catatctaca tacagaaatt gataattaaa aaagtagaaa
      481 ttaaaaaact taatactaaa gatatagata aatatatgag gtaacagcta gagagagttg
      541 ttaccctttt taaatactaa gtatatatta ttcttattaa ttttttgtaa attcactaaa
      601 agaccttttt atagaatatt attttctttt tttattaaat aggtaaatca gtaaaaatgc
      661 aattccagtt ccaattatta ttccaatgat attttctata attaattttt ttaatataaa
      721 agtaaataat aaaaatgcta tacataaacc taaaatatat cctttaaaat aggttgaaag
      781 ttttcttttc atataatcac caacttgtat cagatttagt ttacttaatt agagtataac
      841 agtgttgtaa cttcaaagag tatctaaaag attacaaatg ttagataaaa tcatcaataa
      901 tgtaatttat aacacgtagc tataaactat gcaataatat aaatggaggt ataagtaaat
      961 gaaaaagttc catgttttgc taacagttta aacaaatatt aggtagaaaa gaatgtatat
     1021 aggaatctgt ggtaaaatta tcaatttaaa agtatgattt agtgtgatac agttcttata
     1081 ggtaatggat aacaaataga taaaggaaca gtttctttct tagaacatat tttgttatga
     1141 ctttaaacaa agttaatatt aatgtcaata atatagtaaa agaataaaaa tcatatagta
     1201 taataaaaaa gagtgtttct aaacactctt ttttaatcat tttcatcttc tacatattca
     1261 agtatatcac ctggctgaca gtctaaagct ttgcaaattt cgtttagagt ggtaaatcta
     1321 atagcttttg cttttccagt ttttaaaatt gataaatttg catttgtaat atctattttt
     1381 tctgccaaat ctttaagtga tattttcctt tttgccatca ttacatctag gttaactact
     1441 attgccataa catcacctct atattgttaa atcattatct tcttttattt ttatagcttt
     1501 atcaaatgtt tctgcaatta caaagcacat tagtgcaaat ataaaagaga taaccataag
     1561 tggtgagata ccgccactat cagttccaaa aattcttata ttattagtat ttaaattata
     1621 accatttata cattcaatta tagtgtttat caataaacaa catcccatta ccttaaacct
     1681 ttttacattt tcaagtacga atggggaata cttagtacta ttaattataa atatcaagaa
     1741 taagatgact ataaagtagt caaaaaaata tagtattctt gcaattaaag cgattacttt
     1801 gtcagaacta aaatcatata aaaacatagg tatcatacat aagaatatta taccagcaac
     1861 tcctaaaagt gataatagaa aatatagaaa tactgataat atattactaa aaaattttgt
     1921 aatcat
//
LOCUS       DAENCV010000268         1901 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.272, whole genome shotgun sequence.
ACCESSION   DAENCV010000268 DAENCV010000000
VERSION     DAENCV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.272"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(209..457)
                     /locus_tag="KQD82_003857"
     CDS             complement(209..457)
                     /locus_tag="KQD82_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG4382289.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(488..949)
                     /locus_tag="KQD82_003858"
     CDS             complement(488..949)
                     /locus_tag="KQD82_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG4382290.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(987..1820)
                     /locus_tag="KQD82_003859"
     CDS             complement(987..1820)
                     /locus_tag="KQD82_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382291.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
BASE COUNT          674 a          276 c          213 g          738 t
ORIGIN      
        1 atttgataat gtccaattca tatatgcaaa atttatatac tttaaaatat ctaagaaatt
       61 attcaataaa aaaatactat tttttaaaaa ttatcattat tgataaagct ctactattta
      121 tattttttag tgtattcatg tataataagt aaaagcacaa cggtatatac tgttgtgctt
      181 ttttgtaatt aataaacaat attttggtct ataacaatga aaaatggcta ctttccataa
      241 ttgatttcat ctgacataag caaaaagggt gtccagctgg gtcaatcata actctccagt
      301 catcagtaaa ttgctcctct gcgattgtag ctccacaatg aattgcatat tgaactgctt
      361 cttctaaatc attaacagcg aaatccatat gagccatttg ttgttgagat tcaggttttt
      421 ctggttctat tatgccccaa gcctttgttt tttgcaatat aaagaaaaaa ttatatattt
      481 taatatttta taattgttga aattgtgtaa taaattcaac tggatacata gtgcgttttg
      541 aaatttcttc taaagctaaa ccttgtttta aaagtctctt tatgacgctt tccatacttt
      601 ctgaaatttc tataatattt ttatcaaaat cataaaacct aaaagttctt tgaccccatg
      661 ggtattcttt tatataatgc aaaaattcaa tatttcctat tctatttatc ttactttccc
      721 attgtttgat gtcattaacc tcaaaataca actgaaagtt atttgattgt tgatatattg
      781 gtaaatcata tccaattata gctttataat tactttgtaa tccaaatcca ttttcaaatg
      841 atacatgttc tcctaaatct aatgtgattt tttgctccat aacatcttca taaaactttt
      901 ttgatttatt tatgtctttt acaattagca atactcctcc aaatttcatc ttatcactat
      961 cctttctttg ttataagtat tataacttaa aattttgcaa tttcactgta aaaatcagac
     1021 atattagctc ggtatttttg aggagttatt ccacagacaa ttttaaaatc tttaataaaa
     1081 tgtggtatat catagaatcc taattcatta catatagaca tcaatgaagt tttattattg
     1141 tttagaagtt ttatagattt attaatacgt accaaacgag aaaatgactt catactcaac
     1201 cctaggtact gattaaagag tctgtttaaa tgcctagaac tataaaatac ttttttagaa
     1261 atttcttgtg aagatatatt tccagagttt tgaataatca actgaattgc caaaataaac
     1321 tcatatgagt attcaatttt aatattagaa atcaataatt cttccaactt taaaatcaat
     1381 ttatctatag ataaagattc attaaatatt tttttaattt ctaaatctaa tgaagtatta
     1441 attatagaaa atggaacaac tttatcaata agttcacttt gctgtatacc tataagcggg
     1501 aaaaaaccag caggttgaaa ttctataatt agtataacat cacatctatt agcaatattt
     1561 cctactgttt ttggtttagt agttggacca aataatagac tatgtaaccc tgtggcatca
     1621 taaaaaaaca ccaaagttac acttccatga ggaataatcg tgtaattatc agaaattatt
     1681 gtttgatttg gaaacgatat agtaaaattc gatatccaat ttcttaattg tatacaaggc
     1741 aagagataaa caaaattgtc ttcacaaact ataagtgacc tatttttatt tataaaatga
     1801 atactttctt tttttaacat atattatatc ctgtcctatc gtgaatattt attaaagtaa
     1861 gaagaatcca tattatctct atatatcatt aactgctttt a
//
LOCUS       DAENCV010000269         1812 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.273, whole genome shotgun sequence.
ACCESSION   DAENCV010000269 DAENCV010000000
VERSION     DAENCV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.273"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            122..511
                     /locus_tag="KQD82_003860"
     CDS             122..511
                     /locus_tag="KQD82_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4382292.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEVMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            834..1343
                     /gene="vanZ1"
                     /locus_tag="KQD82_003861"
     CDS             834..1343
                     /gene="vanZ1"
                     /locus_tag="KQD82_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG4382293.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1445..1639)
                     /locus_tag="KQD82_003862"
     CDS             complement(1445..1639)
                     /locus_tag="KQD82_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG4382294.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
BASE COUNT          716 a          179 c          255 g          662 t
ORIGIN      
        1 aaataaatct tatattgcat atggaataaa ttggaaaata tacttatcct aatttaattg
       61 attacatact attattagta taaaatttag ttatacataa attggtaaga ggtgtaaact
      121 aatgaagatt aataaattac cacatgcaga actaaaacta atgaagtata tttggggagt
      181 agatgatgta ttagcctcaa gagatatcat agaagtcatg aagttgaagt atgattggaa
      241 aaagagcaca acattgacat ttttaaaaaa tttagtagat aagggctttt taactactga
      301 taaggtagat agatgtacgc attatactat agctataaaa gagaaagatt acttaaaagt
      361 tgagacaaag agttttttta gttttatgca taacaattca tttaagagtt ttatttcagc
      421 attacatgat gatgaagttt tagatagtaa gagcttggac aaattagaag aatactttaa
      481 aaatttgaaa gaagaagata ttgatgacta ataaagctat cagtatcttt ttagtttgag
      541 acaattgaga gtagcgtttg gatttaatgt tagttttatg tgatggtaga acattatata
      601 aagaaaagtg cttatttcct tagtatctga tagtaaaaaa aaatatagaa aataatacca
      661 attaaaattt aaagatatat gaaatactac aatagcttaa atcatgtaaa tatgataaag
      721 atattgaaat tataaaagga atataatttt agaagaaata gttgacagat aaattacctt
      781 actatattat tgtattaata aaatgataca ataatatagt aaggaggtaa taaatgaaat
      841 ctagaaaaca taatattaca aaaggattat ttatagttta cataattatt ttaacatgga
      901 taatactatt caagttgcaa tttgatatct catcccttga aaccatgaat cttagaagca
      961 taaacttagt accttttgct gggtcactta taattaataa tagagtagat atatcagaga
     1021 ttatattaaa tgtggctata tttgtaccat ttgggatata tgtgtgtatg ctaaaagaag
     1081 aatggagctt tataaaaaaa gttataccta tttttataac tagtttggca tttgaaacac
     1141 ttcaatatat atttgcacta ggtgcaagtg atataacgga tttgatagga aacacactag
     1201 gaggaatcat aggaatagct gtttttatgc tattatcgaa aatatttaag aataatacca
     1261 taaaaattat caatgtattg gcattgattg tgacaattat tgttgtatta tttttaggat
     1321 tagttatttt tgcaaactta tagaaataag tatatatgtt tagcttaaaa tagaacgtaa
     1381 gatacagtta catataaaat tatttacaca taaaaagcac ctatacaggt gctttcctaa
     1441 attattaaag aattattgta tcttgaaact cttgtatgtt caattcttga ttttctcttg
     1501 ttttttctaa taaatctaac gcacaattta tacaaacttt aatttctgca ttggtatttt
     1561 tctcataata agttatatat ttatttctat atttatctat ttcttttcca caacgactac
     1621 acattacaat tacgctcata tcttagttcc tccatctcta attattctta atatattcaa
     1681 tagtatcact tttctgaaaa taatgattgt taaaactgta tcaaatttag atttactttt
     1741 tattataatg cattttaata aaaaaataaa ggttatgggt aaaatattta tagaaaaaag
     1801 tatattattg aa
//
LOCUS       DAENCV010000270         1800 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.274, whole genome shotgun sequence.
ACCESSION   DAENCV010000270 DAENCV010000000
VERSION     DAENCV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.274"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            55..624
                     /gene="mocA"
                     /locus_tag="KQD82_003863"
     CDS             55..624
                     /gene="mocA"
                     /locus_tag="KQD82_003863"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG4382295.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            621..1640
                     /locus_tag="KQD82_003864"
     CDS             621..1640
                     /locus_tag="KQD82_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG4382296.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          715 a          166 c          345 g          574 t
ORIGIN      
        1 tattataaaa atcatagaac tatcaatata ttataaatac caaggatgat atatatgata
       61 aatgcagtga taatggcatc aggctttgcc agaagaatgg gagcaaataa acttttatta
      121 aattataaag gctgttctat aatagaacat gtatttagag aagttagcaa gattgatttt
      181 catcaagttg tagtagtaag tcaatatgaa gaggttttgt tattgtgtaa taaatatggt
      241 tttgaatata tagataataa aaatgcaaat ataggtcaaa gtgaatctat aaaattagga
      301 gttttaaata gctcaaaatg tgatggttat atgttctttg taggagacca gccatttatt
      361 gataacttat atatagataa aattataaat acttttaaat tagatagaga ttttatagta
      421 attccaagat acaaagatat gtgtggaaat ccagtcatat tcccatatag caagaaagaa
      481 gaattgttgg cacttaaaga agatgaaaaa ggtaaaacca ttttaaaaaa ttcatcaaaa
      541 ataaagtatg tagaagttcc taaagaaatg ttatttgata tcgatacaaa aatggactat
      601 gagagaattg ggggtaaatt atgaagctaa taaaaactat agatgctgtt ggacaagttt
      661 tatgtcacga cataactcaa ataatacctg gagaatttaa aggaagaaaa tttaaaaaag
      721 gccacatagt aaaagaagaa gatattccag tgttattatc tcttggtaaa gataatttgt
      781 atgtatggga aaagtcagaa ggaatggttc atgaaaatga aggcgcattg tttttaaaag
      841 aactaactgc tggagaaaat ttagatttta gtgaaataaa agaagggaaa atagatttta
      901 tagcagcatg tgatggtcta ttaaagatag atgttgatgc actttttgat ttgaattgtg
      961 ttggagaaat aatgatggct actcttcata ataattttat agtaaataaa ggtttaaaag
     1021 tagctggaac aagagtgata cctttggtta tagatgagaa aaaattagaa gatgcaaaaa
     1081 aagtagttgg taatagaaaa atagtaaatg tagtaccttt taaacctaag aaagtaggaa
     1141 ttgttactac aggaaatgaa gtgttttata gcagaattgt tgataaattt ggacccgtaa
     1201 tagaagaaaa agtaaaaggc tttggttgtg aagttatagg acagactata tgcccagatg
     1261 ataaagagat tataaagagt gcaataaaag aatttataag tcaaggagca gaacttatct
     1321 gttgtactgg tgggatgagt gtagacccag atgatgttac accaacagct ataaaagaaa
     1381 ctggagctga tttagtgaca tatggttcac caatattacc aggagctatg tttttacttg
     1441 cttattatgg agaagttcca attatgggta taccaggatg cgcaatgtat cacaaaacta
     1501 cagtatttga tatagtttta tctagggttt taattgatga aaagcttgat aagtatgata
     1561 tagctagata tggtcatggt ggcctttgta tgaactgtga tgtatgtact tatcctgcat
     1621 gtaattttgc taaaatataa agttattaaa aaagagtatt tacaaattaa gtactctttt
     1681 taaatatgaa acaaaacagc tagaatgatg ttttaagaaa agcatattta tttagaaatt
     1741 ataataactt attttcaatg tgtaaaagaa tgaataaagt tgcaatttga tttctttata
//
LOCUS       DAENCV010000271         1757 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.275, whole genome shotgun sequence.
ACCESSION   DAENCV010000271 DAENCV010000000
VERSION     DAENCV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.275"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            159..1595
                     /gene="miaB"
                     /locus_tag="KQD82_003865"
     CDS             159..1595
                     /gene="miaB"
                     /locus_tag="KQD82_003865"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG4382297.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          750 a          174 c          307 g          526 t
ORIGIN      
        1 aattatatga ttttatattt tagaaatata gtatatataa atcattagat ttactattaa
       61 aacacaacta gaaaaataat aatatcttta aaagattttt cttatttgat ataattaagg
      121 ttatgtgtaa tataagaaat taactaggag gaatagacgt gagcaaaaga aaacaagtaa
      181 caatacctat agagaaaata caagaacaag ataaatatat agaacaaata cacagacaaa
      241 atgaagaata cttcaacaga actggaaaga gaaaactcgt gtttactcaa accttcgggt
      301 gtcaaatgaa tgaacatgat tcagaaaaat tatgttcaat gttagaagag atgggatacc
      361 aaatgtcaat gatggttgaa gaaagtgatt taataatata taacacctgt gcagttagag
      421 aaaatgcaga gctaaaagta tatggtaact taggacaatt aaaacatcta aaaggaaaaa
      481 atccagatat gaaaatagct gtatgtggat gtatgatgca acaaccacat gttgtagagg
      541 aattaagaaa aaaatataaa catgtagacc ttatatttgg aacacataat ctatataaat
      601 ttccgcaatt acttacagaa tcaataaatt ctgacaaaat gttagtggat gtatgggatg
      661 ttgatggaga agtaatagaa ggtcttagaa gtaatagaaa atttgaatta aaagcatttg
      721 taaatataat gtatggatgc aataatttct gcacatattg tatagtccca tacacaagag
      781 gaagagaaag aagtagaact cctgaagata taattaacga gataaaagag cttgttgcaa
      841 atggaacgaa agaaatcact ttacttggac aaaatgtaga ttcttatggt aagacacttg
      901 aaaatccagt ttcattctca gaacttttaa gaaaagtgaa tgatatagaa ggaattgaaa
      961 gggttcgatt tatgacatct catccaaaag atatatctga tgaagtaata tacgcaatta
     1021 gagattgtga taaggtgtgt gagtttttac atctaccaat acaatgtgga agcagtagcc
     1081 ttttgaaaaa aatgaataga cattatacaa aggaatatta tttagaaata atagaaaaag
     1141 ctaaaaagga agttccaggt attgcttttt ctacagactt aatgattggt ttcccaggag
     1201 aaacagaaga agatttattg gacactttag atgtggttga aaaagttaga tatgattcag
     1261 cttttacatt tatttattct aaaagacagg gaactcctgc tgctaaaatg gaaaatcaaa
     1321 taccagaaga tataaagcat gatagattta atagggtgtt agaagctgta aataggattt
     1381 ctgcagaaat aaatgatgga tataaagata gaatagttga agttctagtt gaaggaagaa
     1441 gtaaaaataa tgaaaataag tttgctggta gaactagaca aaataaactc gtaaatttcg
     1501 aaggtggaaa tgatgattta attggtaaat tagttatggt caaaattact gaacctagaa
     1561 ctttttcatt aaatgggata ttagtaaata attaatattg tacaaaataa taaaattatg
     1621 caaactaatc aaaatagatg tactatagaa caaattatat taaaaatttt tattttaagc
     1681 gaatttgtca tatgttatac taatgtaagt ttttgctatt aataaaaata aatttgatat
     1741 aagatataat ttcattg
//
LOCUS       DAENCV010000272         1619 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.276, whole genome shotgun sequence.
ACCESSION   DAENCV010000272 DAENCV010000000
VERSION     DAENCV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.276"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            268..969
                     /locus_tag="KQD82_003866"
     CDS             268..969
                     /locus_tag="KQD82_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG4382298.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGKHTLDDVQETVNSSYVFDTIIDSLKEKITHNFLRNLHSSLIFNTTLN
                     SRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT          642 a          172 c          207 g          598 t
ORIGIN      
        1 gataaaaata cgttttatat gtttttagat taatataaaa tatcacaatg aaaagttgta
       61 caaagtgata tataataatt atatgaacta taaaattgac taaacttatt gactaataaa
      121 ttagatgaaa agttgagact tactgaaaat aaatacaatg aaatatatac tacaagtatt
      181 aaattaaatc tgaactttac atactattag aataagtatt ataagattat aatgatttaa
      241 aaatattaaa ataattgaga tgataacatg aacaattcct ttttagataa attgattgag
      301 acaaaagaac ttaaaaattc actttataat gtgttaaaac acaattttct ttatcattct
      361 aataagatag agggcagtac ttttactact gaagcattag cacttttact cgataagaat
      421 gttgttacag gaaaacatac tttagatgat gtacaagaaa ctgttaattc tagttatgta
      481 tttgatacaa taattgactc actgaaagag aaaataacac ataatttctt aaggaatcta
      541 cattcaagtt taatcttcaa tacaacccta aattccaggg ggatgtctgg tatatataaa
      601 actataccca atatgatact aggaactgat gtaagcatag cacaaccatt tgaagttgag
      661 ccaaagcttg atgaattaat tgaatggtac tatagtcaga gtgaagtatc tattaaagtt
      721 atagctgaat ttcattatag atttgaattg atacatccat ttcaagatgg taatgggcgc
      781 ataggtagat ttgttatgct aaaacagatg ttagaaaata atcttcctat aaaaattgta
      841 tcttgggata gtgaggactt gtatagaaac tccttaaatt cttgtagttt aggtaattat
      901 gtccctttaa tcgaatattt atcaagttta gaagatttta gagaagtata taaaatgtta
      961 tggaaataat tgtttgcagc aaaaaaaagt ttttatataa atatattttt atctatgcta
     1021 tttaacgctg atttcttagt tattcttagc ttggactata tccactattt taactatata
     1081 aaatagtgga tatagtttaa ctatctcttt ataactattc ttatgtatct tagttgtttc
     1141 ttaatatggg gacagttcat aaaactaaaa gtgattttta attttatcat ttataattta
     1201 ccattacatc agaaaaatac ttatcattag aattttcata aaaggtacct atttttttca
     1261 attaactaaa tcaattctat tagtcttaat ctttcatatt tcaaattgat aatataagaa
     1321 aataaaaaat aaaaaatttt aaatttttta tttattgcga aaaaaataaa aaggtgctat
     1381 actttttgta acatattatt caaattatta agagatagta aacatttata gtttaatttg
     1441 tagtacacta gcatgatttg cagaacaatg aattaatcta tagtataata gtttaattta
     1501 aagtacaacg atttaatcta tagtacaata acttgatttg aagtacaacg atttaatcta
     1561 tagtacaata acttgatttg caatacaatg aattaatcta tggtataata gtttaattt
//
LOCUS       DAENCV010000273         1566 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.277, whole genome shotgun sequence.
ACCESSION   DAENCV010000273 DAENCV010000000
VERSION     DAENCV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.277"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(187..498)
                     /locus_tag="KQD82_003867"
     CDS             complement(187..498)
                     /locus_tag="KQD82_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382299.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(554..1147)
                     /locus_tag="KQD82_003868"
     CDS             complement(554..1147)
                     /locus_tag="KQD82_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG4382300.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          567 a          203 c          197 g          599 t
ORIGIN      
        1 atagaaaatt aatttaaaga aatacaatac attaattttt aagtaacatt aaattaatgt
       61 aagtaaaata atgttcatat gaatatttaa aggtgcattt tctgaagcta agtatatgtt
      121 gtagagtgtt atgtctctct atcctgtgaa gtttaatagc tcaagaaaat gcactattta
      181 tgatgattat tttgttttat ttccaatttt ggaaataact attctaatta aatctaaaat
      241 tataccataa ataagaaaag aaagtataga tgctatgtac caataaatcg aaattgaccc
      301 tgtaattgtt ctacatggga tttgcatacc tgaatccata aaaacaaaaa aactatcaac
      361 tctggaaata atagacaaaa taggatttaa acctattaga acaatacttt ttgagtcttg
      421 accaatttga tgcatataaa tctcaaataa tgcaattaaa aaaaaccaag atgtaaaaga
      481 tttaataatt tttttcatca aaatacctca cataatataa attctgtttg cactatttgc
      541 ttactacaac tatttatctc cattcagtca catcacttaa ttgtaatcta ggtcttcttg
      601 atggttcttc atcaggataa cctaaagata ttgctgccac taattgacta tctgtattta
      661 accattcaca taattcacga tatgcaaagt atacatcaca aatccaaaga ctacctagac
      721 ctaattccac agctgtaagc gacatattct gaattgcagc tccaattgat tgcatatttg
      781 ccatttcata gaatcgttct tcaggggata gcttttctaa aggtgattta cctaatatat
      841 ttaaaataaa aattgttact ggagcttgtt tcatgatttc aactgtgtaa tttgcaccag
      901 cgatatgttg acgacttcct ggaagaagcc cattatcatt aatttcattt tgaatacctt
      961 tgctcatggc tttaagcata gattcttttt ccttttcagt gatgacaaca aaacgccatg
     1021 gttgcctgtt tttagatgaa ggtgcattta ttccagcttc aattattttc tctatagttt
     1081 catgtgaaat gctttgattt ttatattttc tgatacttcg tctttttgat atagaatcac
     1141 ttatcatttt aagtaacctc cgtttatttt ttggaaaaat ctatttaatt tttattttct
     1201 attttaatta ttttagcatt aatcagaaaa aaagtcattg attttagtat acaattatta
     1261 atgattatat taagaattgt atcaaaaagt atgtgattat actaagggat tatattaaac
     1321 attatattta aaattgtatg aaaggttgta tttaatatta tattgagata tatatatatt
     1381 aggatttata tatattcaga tttattatta tattgagtct tatattaaat taaggactat
     1441 aatttgaaaa taagctataa aaacttaata ttgttttgtt cagagataaa aaaagaatac
     1501 tttaggtgat ttttattaaa aattaagagt gataaaatta tatactttat aaatgtaaat
     1561 atgacg
//
LOCUS       DAENCV010000274         1564 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.278, whole genome shotgun sequence.
ACCESSION   DAENCV010000274 DAENCV010000000
VERSION     DAENCV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.278"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            142..>1564
                     /locus_tag="KQD82_003869"
     CDS             142..>1564
                     /locus_tag="KQD82_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG4382301.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYL"
BASE COUNT          638 a          165 c          266 g          495 t
ORIGIN      
        1 ctaaatgaat aattgtatta atatttattt gtaatatgct taatataatt tgcaatttta
       61 cataagtttg ttaagtacat aactaattta agaatatgta tttatatatt aaaaatttat
      121 tatgaggggg acaattttat tatgaaattc tataaaagaa tattaacatt gactttaagt
      181 atttcttttg tatttgcaaa tattcaatta gtagatgcaa tatcatctgt agaaaaaata
      241 caaggaaaaa ataagtatga aatcgcaggt aaaatagcag ataaaaatgc atataaaact
      301 gctatattaa ttaatactag taactctatt gcagatggat taagtgcaag tggactagca
      361 ggagctttaa atgcccctat actattaact gaaaaaaata ctataccaac tgagacatct
      421 gcaagattaa aaaatgtatc aaaagtttat ataattggag gaacatattc tattagtaca
      481 tctgtcgaaa attcattaaa atctaagaaa atgaaagttg taagaattaa gggaaatgac
      541 agaataaaaa caagttataa tgttgcaaaa gaaataaatt caataaaaaa agtcaatact
      601 gttatgctaa ccaatgcata taaaggagaa gcagatgcta taagtattgc atctgtagca
      661 gcaagagata aagcaccaat aattcttact aatggacaaa gtataccttt ttcaactagt
      721 ggtttaaagt cttatgtaat aggaggaaca gcttcaatga gtaccacttt agtaaacaat
      781 actaaatcta ctagactagg ggggagtact cgttttgaaa ctaataaagc aataataaat
      841 aagttttata aggatgctag agagttttat atagctggag cttatgaatt aacaaatgca
      901 cttgtaggat cttctttatc aaaacatggt ccaatggttt tagttaatga tggtagcaat
      961 aaaagcattt taaaaaatgc taagaagata acttctatag gatatataga ttcaaatata
     1021 gtacaacagt gtttaaatat aacaaatgga attggagata ttaatactgg ggttgtaaaa
     1081 aatgttaaac ctactacaaa gactataaaa gatggaatgt ataaggttgg aaaagatatc
     1141 tcagcaggtg aatacttaat aacttcaaac tcaggaagtt atgcttctta ttatgaagtt
     1201 acaagtgata gtacaggtaa tgctgatagt atactttcaa atgatatttt tagtgggact
     1261 agatatatca ccttaaagaa tggtcaatat attaaaattg aggattcaac aatgacatta
     1321 gcaaagtatg ctaaagccca gaaggccaag aatggtaagt ttggaaatgg tatgtataaa
     1381 attggattag aaattcctgc tggagaatat attataatgt caaattcttc tgatgcatat
     1441 tatgaagtta gaaatgatag cttaggtaat gcagagggaa tagttactaa tgatactttt
     1501 tcaggaagaa gatatataac ggttgaggaa ggtcaatatt taatattaaa tgattgttat
     1561 ttaa
//
LOCUS       DAENCV010000275         1548 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.279, whole genome shotgun sequence.
ACCESSION   DAENCV010000275 DAENCV010000000
VERSION     DAENCV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.279"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..600
                     /locus_tag="KQD82_003870"
     CDS             <1..600
                     /locus_tag="KQD82_003870"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG4382302.1"
                     /translation="WIMQGYGTMAKEAIEQLKEYGVDRPTHVFVQAGVGSLAGAVQGY
                     VASIYDECPITVVVEADEADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFE
                     VLKNYSSAFVSAPDWVSAKGMRILGNPLRGDEKVISGESGAVTTGALVSILESEDLKD
                     LREALKLDENSKVLLISTEGDTDPDKYRDIVWNGECQSK"
     gene            933..>1548
                     /locus_tag="KQD82_003871"
     CDS             933..>1548
                     /locus_tag="KQD82_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4382303.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMA"
BASE COUNT          668 a          165 c          312 g          403 t
ORIGIN      
        1 tggataatgc aaggatatgg aactatggct aaagaagcta tagaacaatt aaaagaatat
       61 ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact tgctggtgca
      121 gttcaaggat atgttgcttc aatatatgat gaatgcccaa taacagtagt agtagaggct
      181 gatgaggctg attgttacta taaatcagca gaagctggag atggaaaacc aagatttgtt
      241 ggaggagata tgccaactat aatggcaggg cttgcttgtg gagaaccaaa cactataggt
      301 tttgaagtgt taaaaaatta ttcatcagca ttcgtttcag caccagactg ggtatctgct
      361 aaaggtatga gaatattagg aaaccctcta agaggagatg agaaagtaat ctctggagaa
      421 tctggagcag ttacaactgg agcattagtt tcaatccttg aaagtgaaga tttaaaagac
      481 ttaagagaag ctttgaaact agatgagaac tctaaagtat tattaataag tacagaagga
      541 gatactgacc ctgataaata tagagatata gtttggaatg gtgaatgcca aagtaaataa
      601 aagacttaag gaaaagaaaa acttaacaaa taaatgaaaa atttaaggaa aataaaaact
      661 taaataagtg aaaactttaa ataaaataaa aactaaaaca aatgaaaaat ctaaataaat
      721 gaaaaagtaa agcaaatgaa aaattcaaaa taaaaactaa ataaaattaa ataaaaccaa
      781 ataaaattaa aataaagtct aaataaaaat ttaataaata aaatataaag aaataaaaaa
      841 tcaatagatt aagtgaaatg ctaataaatt aaaaaagtga aatatcaaaa taaaacgtca
      901 acaaactaaa aattatatgg gggaatatca aaatgttaaa tgaaacaaga aaaaaacaat
      961 taactgaagt atgccaagac ttaataagaa atgctagtta ttcaggtcaa gaagaaaacg
     1021 tagtaaaagc tatagaagaa aattttgctt tactaggttt tgatagttgg tcaagagata
     1081 gatatggaaa tatagtagga tgtataaaag gaaataaacc aggtaaaaaa atattatttg
     1141 acgggcatat agatacagtt ccagttccag atgcaagtaa atggagtgtt cctccttttg
     1201 aagcgagaat agtagatggg aaaatatatg gtagaggaac ttctgatatg aaggggcaag
     1261 taagtgctat gatggctgct gtttcatatt ttgcagaaga tactaacaaa gactttgaag
     1321 gtgaattgta tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt tcagctagag
     1381 agataagtaa ggctgttcaa cctgattatg ttgtaatagg tgaatcttca gaattaaacc
     1441 taaagatagg tcaaagaggt agaggagaaa tagtagtaga aacttttggt aaaccagctc
     1501 actctgctaa tccagaaaaa ggtataaatg cagtttataa aatggcta
//
LOCUS       DAENCV010000276         1518 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.280, whole genome shotgun sequence.
ACCESSION   DAENCV010000276 DAENCV010000000
VERSION     DAENCV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.280"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(335..1042)
                     /locus_tag="KQD82_003872"
     CDS             complement(335..1042)
                     /locus_tag="KQD82_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG4382304.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          478 a          268 c          137 g          635 t
ORIGIN      
        1 ataaaatttt tagtcttcat caataccttt tttgctttca ttgttaaagc tattttttct
       61 attagtttat ttcttataaa acaaacattt agtattaaaa tatatatcac acaatagtat
      121 ttgcaattta actttaaaaa aataaaaata cccaactgac ctaagcaact cgattgggta
      181 taaataatct aaaaaatcat ttttgtataa actctttatt taacttacat ttttatttac
      241 cttctacttt aattcaactt atatttctat gcttcttata ctctcattca acttatattt
      301 ctacttatct tatactttta taaattcatc acaattattt agttctcgct atcaaagaaa
      361 ttgtattaaa atactttcca ttctctactt ccatcatttt tttgtcattt atagcatatg
      421 aattatcaca atttatccaa tccttccaag cttcatcatg acaatttaaa gcttcacatt
      481 tctctacaat cacagactcc gattcagacc aaagtttatt ccaccatgta attgaatgga
      541 agttcatatt atcttgccaa aatggaatga gttcatctgg tatacaatcc acgaaatctt
      601 cttttaatcc aggcattgcc atagcaagta ttcctccctc ttttacaaga ggtgatatat
      661 gattgtctaa aaatccttct ttgtttccaa agtagtgata agaatcgata cttatcaccg
      721 catcaaagaa accttctgca taaggtagtt catgtgcctc tgcctgtatt ggaaatattt
      781 tatcatccaa tttaaactct ttaaatcttt tatagttttc agttggttct atccataaat
      841 ctgttgcaaa tacagtagca tcaaattcct ttgctaaaaa tatcgaagat atacccttac
      901 cacaacctaa gtctaaaata cgcattccct tttctagttt tatcttactt gttaattctt
      961 ccaatatttt tatacaatta ggtcccatca tatttttcat gagaaagtcc ttatcgtatt
     1021 tattactttt tgtaaatatc attctatctt tcctttccct atcagttatt atttattaaa
     1081 ttttatacgt ttgatttttt attttttata tgcttcttat attgagcaat ctctttttta
     1141 ttcattcttt aacagacaat tttaatgtgt aattttatcc actctgtgat agtaacttta
     1201 atgaataatc ttatctactg tgatagttaa ctttgatggg taattttatc tactccacag
     1261 caatcgcttt aatgagtaat cttatccact ctatgatagt tattttagtg agtaatttta
     1321 tctactctac agtaatcgct ttaatgagta atcttatcca ctctataata gtcactttaa
     1381 tgagtaatca taatttttat atcattttag atacttactc taataagaag tcattttcta
     1441 atacttaata agttttaatt acttccactt taacctatat actaattaac ttatttaatt
     1501 tagtgcatat ttgacact
//
LOCUS       DAENCV010000277         1466 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.281, whole genome shotgun sequence.
ACCESSION   DAENCV010000277 DAENCV010000000
VERSION     DAENCV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.281"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            32..643
                     /locus_tag="KQD82_003873"
     CDS             32..643
                     /locus_tag="KQD82_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG4382305.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            941..1279
                     /locus_tag="KQD82_003874"
     CDS             941..1279
                     /locus_tag="KQD82_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG4382306.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSNKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          577 a          162 c          194 g          533 t
ORIGIN      
        1 atttttaata atattatttt aggaggtaaa aatgataaac gatattaaaa ataaattcaa
       61 aaattacaaa ccatatataa atggatacaa gaatatgaag agagcatctg ttttaattcc
      121 aatagtagaa ataaataaca cacactatat attatttgaa gttcgctcaa aaaatttgaa
      181 gcatcaacct agcgaaattt cttttccagg tggtaaaata gaatcaggtg agactccata
      241 tgaagctgtt attagagaaa cttgtgagga acttggaact tttagtgaca atatagaagt
      301 aatatcaagt cttgacctcc ttataacacc agtaaagttt atcatacatc catacttagg
      361 atatataaaa aatattaata atttaaatat aaacaaggat gaagttgacc atactttttt
      421 ggttcctata aaatatcttt tagagaatcc tccaaatact tatagtaata ctgtaaaagt
      481 ttttccagat gaaaatctac catatggttc aattccaaac caaaaagatt ataaatttga
      541 aagtggaagt tatcctattc tgttttataa atataaagat tatgtaattt ggggaatgac
      601 agcaaaaata ctagaaaact ttttaaattt tttatcagaa taacactcat aataaatcat
      661 caaaactttt acataagaaa tttataaaag tttctaaatt taacaattct aaaatatcaa
      721 taactatatt ttgtagcact tttttatttt attattgttt gaatatttga tggttttcaa
      781 tgcaatttca acataaatac tatgtattag tgtatactgt tgatgaatta aagtatgata
      841 atctatttaa taaagtatga agtaaatgat tttaatgtgt ctttatggta aaattactaa
      901 taagtaataa aattaattaa aatatatgga ggtgcttata atgtttgctg taggatttgt
      961 tgttataatt gcacttgcct ttttcatttt aagtgtacta ttatttaatg gaaaagccag
     1021 tatgctacta gcaggttata actctatgag taataaagaa aaagcacgat acgataaaaa
     1081 aaaattatgt agaagttcta gcattgttac ttttattgtc tctataatgc tatttattat
     1141 ggcgtttttg ggatatagaa tagagagtgg tcaaatggac gaaaaaaaaa tggttccctt
     1201 ttctataata tttataatag tcatactttc cagtgtattt tttaatatcc tctactctaa
     1261 caaaaaatgt aaaaaataaa tagttgtaag ttgagcagtt aattataaaa gattgactgc
     1321 tttttcatgt atattgacta ctcttattaa atattttaac atttaaaaat ctatgtaaaa
     1381 ataatgaata tagtacaaac ttctaataat gattttacct ttgtggttac agtccactat
     1441 atataattta atatgataac agggaa
//
LOCUS       DAENCV010000278         1461 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.282, whole genome shotgun sequence.
ACCESSION   DAENCV010000278 DAENCV010000000
VERSION     DAENCV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.282"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            124..960
                     /locus_tag="KQD82_003875"
     CDS             124..960
                     /locus_tag="KQD82_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG4382307.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            981..1403
                     /locus_tag="KQD82_003876"
     CDS             981..1403
                     /locus_tag="KQD82_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG4382308.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
BASE COUNT          604 a          187 c          226 g          444 t
ORIGIN      
        1 acctactgta agtataatat tatattgaca tgaattgaca ttattgtctc ttagtcaata
       61 ttatacaaga agtataaaaa aatatgtgtt acactttgta taacatgaaa ggaggttacc
      121 taagtgagac aagaagaacg cacagtatgc tttgataggg aattaaaaat tgaagcatat
      181 agatttaaag gcataatgca aaaattccca aaccattttc atgaacatta tgtaattggt
      241 tttatagaaa aaggtcaaag atatttatct tgtaaaaata aagagtatac cacaagtact
      301 ggagatttgc tactttttaa cccttttgat agtcatactt gtgaacaaat agatgataaa
      361 gtacttgatt acagatgtat taatattaag ccagaaataa tgaaaaaaac cgtctttgaa
      421 attacaggta aaaattattt accaaaattt aatcaacctg ttatatttcg cagtgagcta
      481 gtacctctac tacaagaatt acactatata atcatggaag aagaattgga ttttaaaaag
      541 gaagagcttt ttttcttcct aattgaacaa ttaattgaag aacatacaga gccaaatttg
      601 caatctaact tggaaaatac aaatatagaa attcaagcag tatgtgatta tctagaaaat
      661 aattatgctg aacatatagt acttgacgaa ttaagcacta tagcaggtat gaataagtac
      721 tctctacttc gtaattttac gaaattaaaa ggtattacac catatagata cctagaaaat
      781 atacgggtaa acaaagctaa aaagctttta gaaaaaggcg tagaacctat agatgctgcg
      841 atacaaacag gatttgtaga ccaaagtcac tttacaaact tttttaagaa ttttattggt
      901 cttacaccaa agcagtatca aaatatattt ataaatgatg attcaaacaa ctttaaataa
      961 ggtaaaaagg agaaataaaa atggaaaata aaaattcaaa atgtgtcatg gtaatagacg
     1021 aaaatttacc aatgggaatt atatccaata cagcagctat aatgggaatt acactaggaa
     1081 aacatgcccc agaaactgtt ggcccagatg taattgacaa aacaggtaat agccacttag
     1141 gaattattga cattcctgta cctattttaa aaggaaacaa agaaataata aaagatttac
     1201 gtaaaaaatt atatactttg gaatttaacg atttaactgt tgtagatttt tcagatgtag
     1261 cacaaagttg caatctttat gaagaattta cacaaaagat tgctagtgtt ccagaagatg
     1321 agttacagta ttttgggata gcaatttatg ggaataaaaa gaaagtgaat aaacttactg
     1381 gtagtatgcc tttacttaga taattgttaa acaaccctat atatgatatt aaatcaaatg
     1441 atattttaag atacataaac t
//
LOCUS       DAENCV010000279         1415 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.283, whole genome shotgun sequence.
ACCESSION   DAENCV010000279 DAENCV010000000
VERSION     DAENCV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.283"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..130
                     /locus_tag="KQD82_003877"
     CDS             <1..130
                     /locus_tag="KQD82_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG4382309.1"
                     /translation="KEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            185..322
                     /locus_tag="KQD82_003878"
     CDS             185..322
                     /locus_tag="KQD82_003878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBG4382310.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
     gene            complement(308..619)
                     /locus_tag="KQD82_003879"
     CDS             complement(308..619)
                     /locus_tag="KQD82_003879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382311.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSVNSLINNAVHSFCISHWLIY
                     L"
     gene            740..910
                     /locus_tag="KQD82_003880"
     CDS             740..910
                     /locus_tag="KQD82_003880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382312.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            913..>1415
                     /locus_tag="KQD82_003881"
     CDS             913..>1415
                     /locus_tag="KQD82_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382313.1"
                     /translation="MNNLQIFEKLEFGQIRMVEVDKKPYFVATDIAKCLGYANTSKAI
                     NDHCRWVTKSYIPHPQNENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPT
                     IRKTGGYIHTTEDMTDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADSV
                     ASSDNSIL"
BASE COUNT          595 a          172 c          250 g          398 t
ORIGIN      
        1 aaaagaaggt acaagagtac atccagatgg tcatacaagt attactaaaa cacctaagat
       61 tactggtaag ggacaaatat acttcattaa taaatttaaa agtagcaaac aattatcaat
      121 gttaagttaa aacataacag taccttgaaa actaaataca gaatattcaa aagaggtgat
      181 tgaattgaaa cacgagcaaa caacgcttcg tataccagaa gacttgtaca aagcgttgat
      241 agatttaagt aaggaaatcg gaatgcctat tgcatctatt gtaataattg cgtgttggtt
      301 gtatatatca aagataaatt aaccaatgag atatgcaaaa tgaatgcaca gcattattta
      361 ttagagaatt gactgataaa ttattttctt tagctgtttt ctctagcaac tcttttagga
      421 caccaggaat cctaagagta gaacgaacag tatcatcact tttatacgag attgattgaa
      481 gttcattaac ttttgaattt ctaataatat cattaatagc gtatagaata agcgaagatt
      541 tgagaatgcc tgtttgataa gaaatttttt ctaaattttc atttaatggt gtatatattc
      601 ttacagtaat agtagtcatt ttgacacctc cctaatgaca ttatacaaaa aatatttaaa
      661 aaagtcttga cactaaaata gtgtcataat ataatgatta tagacagtga ataagtgtca
      721 taaggaggga ggaataagaa tggaaaaagt aagaatgaca gtaaggctcg tcccaaaatt
      781 aaatcaatat gtacagaata tagccaaaga atgtggaaaa agtaaaaatt ctattattgt
      841 ggatgcatgt tgggagttta ttgagaaaat aaaaaaagaa aataaattca atgtagaaag
      901 cgaggaataa atatgaataa tctacagata tttgaaaaat tggaatttgg tcaaataaga
      961 atggttgagg ttgataagaa accatatttt gttgcaacag atatagctaa atgtttagga
     1021 tatgcaaaca caagtaaagc aataaatgac cattgtaggt gggtaacaaa aagttacata
     1081 cctcatccgc aaaatgaaaa taagatatta gaagtaaata caatacctga aagtgatatg
     1141 tatagattga ttgtaaatag taaactacca aatgcagaaa aatttgaaag ttgggtattt
     1201 gatgaagttt taccaacaat acgtaaaact ggaggatata tacatacaac agaagatatg
     1261 acagacgacg aaataatggc aagagcatta caagtagctc aaaagacaat agaaaagaaa
     1321 agcagagaga tagaagaaaa agataaggta atccagttac aacaaccaaa agtactgttt
     1381 gctgattcgg tagcgtcttc tgacaattca atcct
//
LOCUS       DAENCV010000280         1415 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.284, whole genome shotgun sequence.
ACCESSION   DAENCV010000280 DAENCV010000000
VERSION     DAENCV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.284"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            97..342
                     /locus_tag="KQD82_003882"
     CDS             97..342
                     /locus_tag="KQD82_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG4382314.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(560..829)
                     /locus_tag="KQD82_003883"
     CDS             complement(560..829)
                     /locus_tag="KQD82_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG4382315.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          567 a          213 c          155 g          480 t
ORIGIN      
        1 tataataaaa aattaaatat taaatattaa ctataataaa aaaatatctg gtaataggta
       61 taatataaaa agaatatacc agaaagaagg tgaagtatgg gagaactaca aaaaattcca
      121 ggggtaggga aagcaacaga aaaatcttta atcatgttag gatatacaac tattaaatca
      181 cttaaagatg caaacccagc acaaatgtat gaaaaggaat gtttaatgcg tggacagcac
      241 atagacagat gtcagttata tgtatacaga tgtgcagtat attttgcatc aactgaaaat
      301 ccagaaccag aaaaattaaa gtggtggtat tggaaagatt aaaaaatcaa aaaagttaga
      361 cataaaaaag agttgaatat ccgttttaaa taataaaaac taatactcaa ctctttttta
      421 gtcgtaaatt ttatgtaaat ctatttgtat ggctaaatat acccatcaat aaatgtataa
      481 atatactaat caacaaatga ctaaacatac tacctaacaa aggattaaat aaattcctta
      541 acaaagcatt ctaaaacaat taattagaaa ctgtttcttc aagccatttt ctacattgct
      601 caatatttac catagggtca aaaccttggt cttttccctt atttaaaagc atatcacaag
      661 ggaaagtatc acattcacca caatgattaa gttccttact ttcacagcaa gttttaacct
      721 tacattctcc accccaaaat ggtttttcca tagttgggca accagtgcaa tttacttgtt
      781 ctttttttgt acaactgtca caacagacac cacatcttga ttcaaacata ttcaaacatc
      841 ctttccatta tttaaaaata ttataaaatg attttagatg aatacaaaag tgcaaatagt
      901 tgattttagg tcatctaaat gttaaacata aaattattag tactggtaaa tttataaaaa
      961 ctattccaaa aatctttttg aatatttcaa aacacaataa attacttcaa atccccttga
     1021 atatatttta cagcattata gattgcttta aatctctttg aatattctac aagactatag
     1081 attagtttaa atccctttga atatatttta caacattata gattgtttta aatctctttg
     1141 aatattctac aagactatag attagtttaa atccctttga atatttcaaa acaccataga
     1201 ttactttaaa tccctttgaa tgttctataa cactataaat tacttttaac ttttatataa
     1261 ccataaagtt tttatctggc actttaaatg gagttttata tcaaatttgc cttccatcga
     1321 ataaccaaac ttaatttaat tacacattcc aagattcaaa tattcattca atatatttga
     1381 taattctatg ttatagctat aattaaatat agatt
//
LOCUS       DAENCV010000281         1405 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.286, whole genome shotgun sequence.
ACCESSION   DAENCV010000281 DAENCV010000000
VERSION     DAENCV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.286"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(73..1251)
                     /locus_tag="KQD82_003884"
     CDS             complement(73..1251)
                     /locus_tag="KQD82_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG4382316.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYRLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYINNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT          498 a          148 c          171 g          588 t
ORIGIN      
        1 aaattataat gatagagata ataagataaa aaacatagtg agactacaag taagctcatg
       61 taaatattca ttttagttat tgttcctttt atgattattt tgtttattag tgtacattct
      121 aatatcagca atattaataa tttcttctac agtcattttg ttttcgctag aaacttctac
      181 aataccatag ctaaaagata tcgtattatc cataatttta cttattttct ctataaattc
      241 ttttgtggtg ttttcattag atttaggcat aataactata aactcatctc ctcctattct
      301 agcaaatatg tcatctattc ctaagttttc aataactata cttgagaagt taataatata
      361 gttatcccca gctaaatgtc catatgtatc atttatcgtc tttaatttat ccaaatcgat
      421 atatatcaat gaaaataata aattgttatt aatgtaatgc tgtatttttt caaaagcaaa
      481 ttttcgatta tatgctgatg ttaattcatc aatgttagct tttttcttta ataaatttac
      541 ttttagtaat tgttcttgtt ttatcatttt tagtctatga tgttcttttt ctaaataaat
      601 atttttttca atggaatata tatgattgat gaatataatt agttgtaaga ttaataatat
      661 gttacttccc aatataaagt aggaagttag agaacttggt ggagcaaatg taatttgtat
      721 actatcaaat aatatatatc ctaaagtgaa ccagataaat tttaaaaata gactgaactc
      781 tatttgattt tgagttatta tataaatatg ctcttttatt ttattcatag agtaccataa
      841 gataaaatct aaaatagaag ctattaaaat actaattaac ctataggtca tatttgcaaa
      901 tattatatct ggacttgtat ttttaacaag tgatattgta cttacagtaa taaggtgtaa
      961 aatacaaaaa cttattactg ccatattggg aataaaatgg ttgttagtag aactttttct
     1021 gcttaagagc aatttatgac ttactatgta aaacaatata taatatagtg gtaatggtaa
     1081 ttttgtgaat attattatca agaaggttaa tggttttatt aaaaaaatat atttgtaatt
     1141 cttatgtata gttatatttt tgtttccaaa agatgctatt ttgcatatgt aaatgttaca
     1201 tagttcgatt attaatatag tagttatgat taaaaatata tggtatttca tagtacatcc
     1261 tcttttaaag tgaaattgta tttacttaaa atagattaga ttttatatat tctattttac
     1321 agtttttatt taggtacatg caaagagctt attatggata ccttcttttt tattgcatgt
     1381 gtctgtgtta tataaaattt aaaaa
//
LOCUS       DAENCV010000282         1381 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.287, whole genome shotgun sequence.
ACCESSION   DAENCV010000282 DAENCV010000000
VERSION     DAENCV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.287"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            40..438
                     /locus_tag="KQD82_003885"
                     /pseudo
     CDS             40..438
                     /locus_tag="KQD82_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            426..1364
                     /locus_tag="KQD82_003886"
     CDS             426..1364
                     /locus_tag="KQD82_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG4382317.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
BASE COUNT          497 a          169 c          188 g          527 t
ORIGIN      
        1 taaatttaac taatttatat aaaatcttaa ggagttgcca tgataaaaaa aatagagcat
       61 gtggtcatta ctaataataa tatagaaaat tatatcaatt tttataaaaa atttggtttt
      121 tcatctaaag aaatgagcaa aaattataaa cttaatgcta gaaatgttaa gataaatctt
      181 aacataaaag gttataaatt tttaccttat gttaaaaatt caaaagcaag tagagataac
      241 ttatgtttta aaataaacac aaatattgat gagccaaaaa aatacttaga acaaaatgag
      301 attcaaatta agtgaggaat tgtagaatgt acaggtgtct ttggtaatat gaaatctatt
      361 tatttgaaaa aaactaatgg aaatttaatt gaattatcaa atcatagaat cacggagagt
      421 aaaccttgga aaatttaatc ctatcaataa atgttgttct tcctttgttt ctcacaatgt
      481 cacttggata tgttctaaaa aaattaggtc tatttgatac tcatacttta aataaaatga
      541 ataatgtctg cttcaagtct ttcttgccat tattattatt ttttaatata tacaatacag
      601 atttagatgg ttctcttaat tttaggctta tggctgtagc tgttataatt attttttcaa
      661 tcttcttaat cttagtcttt attattccta aaattgaaaa agataataag acaaggggtg
      721 cattaataca aggtgtattt cgtagcaatt tcgttatatt tggtattcct gtgacaattt
      781 cactatttgg cgataagagt attggagtca catctttatt aattgcagtt attgtaccta
      841 tgtttaatgt attatctgtt gtaattcttg aaatttttag aggtggcaaa gtaaatataa
      901 aacatatgat taaaagcgtc ttaacaaatc cactcataat cgcctctttt ataggattga
      961 caatgctaat tttaaatatt aatataccta catttttaca caaaactatt ggtgatattt
     1021 ctaaaatagc tactccttta tcacttataa ttctaggtgg ctcttttagt ttttctgcta
     1081 taaaggggca tataaacccc attatattgg gcgtatgtgg aaaacttata cttgtacctg
     1141 ctatattttt accaattagc atttttcttg gatttagaaa tgtagaatta gcttcactta
     1201 tgataatgtt atctgctcca actgcagttt cttcatttac aatggcacaa cagatggatt
     1261 ctgacagtga attagctgga caaattgtag tatttacttc tggattttct ataattacag
     1321 tatttttaat aatatttatt ttaaagcaga ataatcttat ttaatgatat ttgataacac
     1381 a
//
LOCUS       DAENCV010000283         1344 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.288, whole genome shotgun sequence.
ACCESSION   DAENCV010000283 DAENCV010000000
VERSION     DAENCV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.288"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(21..1274)
                     /locus_tag="KQD82_003887"
     CDS             complement(21..1274)
                     /locus_tag="KQD82_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG4382318.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT          423 a          254 c          117 g          550 t
ORIGIN      
        1 aatttaagaa cattattaca ttatgtatat attcttattt ttttaattct atttttttct
       61 atactttcta tacaaaatct tatattatca acttcaatta cttcattttc ttctggcaat
      121 ctgcttaggt gtccaattat aaaacctcct atggaatcaa actcctcaga ttccaagttt
      181 acaccaataa gttcattaac atcaccaatt ttagtacttc catctacaat atactcatct
      241 tcttttataa cttgtatttc atcctcttct tcatcatatt catcctctat atctccaaca
      301 atgacttcta ctaaatcttc tatagttaaa agtccagatg ttccaccata ctcatctaca
      361 actatagcca tctgactttt ttcaagtttc atctcttcaa gaagttgtgt tatctttttg
      421 aactcatacg tgaaaaaagg ttctctcata taatttttaa tatcaaatga ctctatttct
      481 tcatcagata aaaatataat atcttttata ttaagtattc ctacaatatc atctatagtt
      541 tcttcataca caggcattct gcttaatttt tcagttttaa atacctgtat tatctcatca
      601 taactatctt ccatatctat agctaccata tctattcttt gtaccattgc atttttagct
      661 tgcatatctc caaattcaaa aacattattt atgatttctc tctcttccat ttcaaggacg
      721 ccttcttcat gacttacatt taccatagtt tttagctctt cttcagttat gaatgattta
      781 gctcctttat ttgttatacc aaataattta aatataatat ttgtaattat attaaatatc
      841 catactattg gtcttaatat aaatataatt gcttttatag gtttagatac taatagagaa
      901 accttttctg aattatttgc tgctattgtt tttggagtaa tctcaccaaa tattaatact
      961 aatactgtca taacagctgt agcaagtgcc actccttttt ctcccattaa tcctataaac
     1021 aaagatgttg atatggatgt tgctgctata tttacgacgt tatttccaac caatatagaa
     1081 cttaaaagct tatttggatt ttctattaat gaacttacta acttagctcc tttaactcct
     1141 tcatcttgca tatatctgat tctgatttta ctcaacgaca ttaaggccgt ttcagatgct
     1201 gagaaaaatg cagaccctat aagtaatact attaaaaaaa taatctgaat caaattattg
     1261 ggcgattcca acacttacca cactcctact tacatgtttt tatattaata ttaattataa
     1321 ataactttaa tattagtttt tatt
//
LOCUS       DAENCV010000284         1342 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.289, whole genome shotgun sequence.
ACCESSION   DAENCV010000284 DAENCV010000000
VERSION     DAENCV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.289"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..600
                     /locus_tag="KQD82_003888"
     CDS             <1..600
                     /locus_tag="KQD82_003888"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG4382319.1"
                     /translation="WIMQGYGTMAQEAIEQLKEYGVDRPTHVFVQAGVGSLAGAVQGY
                     VASIYDECPITVVVEADEADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFE
                     VLKNHAAAFVSAPDWVSAKGMRTLGNPLNGDEKVISGESGAVTTGLLVAAMEREDLAD
                     LRKDLKLDENSRILLISTEGDTDPDKYRSIVWDGEYPSI"
     gene            727..>1342
                     /locus_tag="KQD82_003889"
     CDS             727..>1342
                     /locus_tag="KQD82_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG4382320.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMA"
BASE COUNT          509 a          151 c          303 g          379 t
ORIGIN      
        1 tggataatgc aaggatatgg aactatggct caagaagcta tagagcaatt aaaagaatat
       61 ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact tgctggtgca
      121 gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt agtagaggct
      181 gacgaagctg attgttacta taaatcagca gaagctggag atggaaaacc aagatttgtt
      241 ggaggagata tgccaactat aatggcaggg cttgcttgtg gagaaccaaa tactatagga
      301 tttgaagtat taaaaaatca tgcagcagca tttgtttcag caccagactg ggtttctgct
      361 aaaggtatga gaacattagg taatccttta aatggtgatg aaaaggtaat ttctggagaa
      421 tctggagcag ttacaactgg tcttttagta gcagctatgg aaagagaaga tttagcagat
      481 ttaagaaaag atttaaaatt agatgaaaat tcaagaatac ttttaataag cacagaagga
      541 gatactgacc ctgacaaata tagaagtata gtttgggatg gagaatatcc aagtatataa
      601 ggaattacta aaatgcaatt attagatatt ggtttacata tttagaagat atataagttc
      661 taaaatttag aaaatatata agtatataaa atacatacat tataaaaaat ctagggggat
      721 aatgaaatgt taaacgaaca aagaaaacaa gaagtaactg aaatatgcca aaaattaatt
      781 caaaatccta gttcttctgg aaatgaagaa ggtgttgtaa aagcaataga agagtctttt
      841 gaaaaattag gttttgatag ttggtcaaga gatagatatg gaaatatagt aggatgtata
      901 aaaggtaaca agccaggtaa gaaaatctta tttgatggac atatagatac agttccagtt
      961 ccagatgcaa gtaaatggag tgttcctcct tttgaaggaa aaatagttga tggaaagata
     1021 tatggtagag gaacttctga catgaaaggt caatatactg caatgatgtc tgctgtagcg
     1081 tattttgcta aagacactaa tagagatttt gctggagaac tatatgtagc aggtgttgtt
     1141 catgaagaaa tatttgaagg tgtttcagct agagagataa gtaaggctgt tcaacctgac
     1201 tatgttgtaa taggtgagtc ttcagagtta aacttaaaga ttggtcagag aggtagagga
     1261 gaaatagtag tagaaacttt tggtaaacca gctcactctg ctaatccaga aaaaggtgta
     1321 aatgcagttt ataaaatggc ta
//
LOCUS       DAENCV010000285         1337 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.290, whole genome shotgun sequence.
ACCESSION   DAENCV010000285 DAENCV010000000
VERSION     DAENCV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.290"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            488..1168
                     /locus_tag="KQD82_003890"
     CDS             488..1168
                     /locus_tag="KQD82_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG4382321.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          587 a           83 c          199 g          468 t
ORIGIN      
        1 tattataaaa ataaaagtga tatattgtgt aataatcgaa attttatact ttggttaaaa
       61 taattttata tatagtgcct aatgtataac ctaatgctcg tagacaataa tgtggatatt
      121 atcaaattta tttttataat agaaatttta atttatatct atattaaatt gttaaagata
      181 taattatgag aataaaaaat atagctttga attatataat tgtatatgct tatctttata
      241 cagtaaataa aaaatcaaat gaaataccaa ataaagtgta tatcttaaaa taacttacta
      301 gagatacaag gatgctatgt gaattttgaa gtgtgcagtt tagataataa atatatataa
      361 ataataaaga aaatggattt atagtattta aaatttctta taagggtaaa aaatgctatt
      421 agaaaatgta ctaaaaatat ttttccaatt ctttatagaa aaataatgat atttaggaga
      481 gattgtaatg caaattgatg tattgataat tgatggagat aagaatagtt ctcaagagct
      541 gaaaaatttt ttagaaggaa aggggataaa tgtctattta gcatataatt gtaaagatgc
      601 tattataaaa atatttagca agaaatttga cttaatattt ttagagataa ttttagttga
      661 tggtgatggt tgggattttt gtaaaaaaat tagaaatata actacttgcc caattattca
      721 tataacttat ataaatgaaa atcaaagtat tttaaatgct ttaaattctg gaggagatga
      781 ttatttaata aagcctttaa atttagaaat attatatgcg aaagtgaaag ctattttaag
      841 aagaagagat ttatttttaa ataataataa aaataatata aaactagaag attataatag
      901 agctagtgga gtaataaagt tagaaaataa aatgataaag ttaactccaa cagaaaatag
      961 attattaaat tactttatag agaatgctga aaagactcta actattaagg agatatatga
     1021 acatgtatgg atgaatgagt atttagatga taattatagt gtagtggttg ctgtgagtgg
     1081 attaagaaag aaaatagaga aagattataa aaatcctcaa aaaattataa cgataagaga
     1141 agtagggtat tactttaaca agatataaag ctttataaac agaatgaatg ataaggcata
     1201 gataatatgt tagtatatta caagagtttt tgttctaacg atatatttgt aaaattagat
     1261 ttaatttact taattaaatt atagcagtag aataaatata aaaaatgtct gaaatattat
     1321 agacatcagt aaaatat
//
LOCUS       DAENCV010000286         1259 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.291, whole genome shotgun sequence.
ACCESSION   DAENCV010000286 DAENCV010000000
VERSION     DAENCV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.291"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            235..945
                     /gene="rgaR"
                     /locus_tag="KQD82_003891"
     CDS             235..945
                     /gene="rgaR"
                     /locus_tag="KQD82_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG4382322.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          501 a          100 c          198 g          460 t
ORIGIN      
        1 atatagggta ataaatacat attatattta ttttactata tttttttaca taatatatct
       61 agaaaagtat aggaatttat cgtaatttaa gtttaaatat taaagagaat ttctataagt
      121 agtttaatcg acaatttaaa tttataaaga aatttaaatt tgaaagctag tgagtacagt
      181 ttggaaattg aaataaagta aactaaatga tattttagat ttggaggtga tattatgttc
      241 agggtagtta tatgtgatga cgaaaaaata caaagaagca tactaaaaga gtttacagaa
      301 aaacttttgt ctgaaagatg tctaaattat caaatactgg aattttcctg tggagaagat
      361 ttaatatcca aatacccaga aaaaattgat attatatttt tagatatcca aatgaaagat
      421 atcaatggaa ttgaggtggc taaaaaaata agaaagtttg atgaaaaagt agaaattata
      481 tttacaacgg ctttttcaga gtatgccccc caaggttatg aggttcgtgc ttatagatat
      541 ttagtaaagc ctatagagta tagtaacttt gcaatgggtg taaatctttg tattgataac
      601 cttcaaagaa agaaggagcg ttatatagtt ttaaatagta aaaaaggatt taatagaata
      661 ctgatagatt ctattttata tgttgaaact gtaaagagag atttagtcgt acatacaata
      721 ggtagagatt ataaagctaa tatgagcatg aaacaagcag aaaaattact gtgtgaaaat
      781 ggctttttta gatgtcatac aagttttatt gttaatttaa gaagaataga agatcttagg
      841 gataatatga ttactattaa tgaacaattt atacctataa gtaagtatag acttaaagat
      901 ttaaaagtag cattaacaag cttgttatgt gatataatgt attaatttta aaaaggtaat
      961 tgaaaatatc gtaaaaaaga tttaatgtca tcacagggct attgattgaa actgtcttaa
     1021 tatataaaat gttatttata ttaagacagt tttattatgc aaaatagtag tttatagatt
     1081 tttaatatgg tttaacatgt atatgtagag tttccattat aatagcatga ttattaatta
     1141 gtagcttgat tatcaatata ctattattga tttttatatg cattatatgt atttttagaa
     1201 tatgattacc tttttaaaat aatgactatt tataaaactc tatagatatt tgtttcatt
//
LOCUS       DAENCV010000287         1226 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.292, whole genome shotgun sequence.
ACCESSION   DAENCV010000287 DAENCV010000000
VERSION     DAENCV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.292"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            332..706
                     /locus_tag="KQD82_003892"
     CDS             332..706
                     /locus_tag="KQD82_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4382323.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            811..1184
                     /locus_tag="KQD82_003893"
                     /pseudo
     CDS             811..1184
                     /locus_tag="KQD82_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          556 a          109 c          172 g          389 t
ORIGIN      
        1 tcataaaaac aaaaataaaa tatgctttta gtaacttgtt aacataactt ataatttatt
       61 ttataagcat tagcctatat agctttgtgc tactaaatct ctttgattct aacgatttag
      121 tgctattcta caactaaaat aatacaatag agctatttat tgtttggtag cattgtttgt
      181 aaattaaaca aatttataca tatgaatatt attttacatt caaaaaaaca gactaaatat
      241 gtagaatatt tggtaaaact tacctctagt ttttgtctta aaaagctcct attattaact
      301 taagttagaa gaattaagga agtgacataa attgaaagaa gaaatattaa tagaaaaatt
      361 attaagagca gaattaatag taatggagta tttatggaaa aaagacactc taatgcctaa
      421 aaaagaaatt gtaaaagaaa taaaacagat ttatggatgg cataaaagta caataaaaat
      481 actactaaaa aggctagttg ctaaaaggta tttggctaga tatattataa attctcaatc
      541 tcattataaa ataatagata gtaaagaata ttatactttt aagaaaaagg tactaaaatc
      601 tagtaaaagc ataaaaataa tgcgttcact taccactaca cataaaaatg taagtaaaga
      661 gaagttagac ctattagatg agtattatag aaacttaaaa gaatgatata gtaaaaatcg
      721 aatgtaatta tttaccttaa ttcttttgtt tgtaaaactt tattgataca gaatttaaca
      781 tatacataaa ttggtaaagg aatgtatact atgaaaatta gtaaattacc agaagcggaa
      841 ttaaaagtta tgagatatat ttgggaatca gaaaaggtat taatatcaag agaaatcacc
      901 aagtctatgg aacaaaaata tgaatggaaa gatacaacta tatttacagt attaaaaaga
      961 ttactaaaga agagagtttt tagaagcgga gaaaatagat aagtgtacac actataatgt
     1021 attagtaaaa aaaataatat cagagatttt aaacaaaaga atttttaaag aatacacata
     1081 aaaattcaat attaagttta atgtcagcac tatgtagtaa ggatgatgaa gtagatggtg
     1141 atattttaaa tttattagaa gaatatttta agaatttgaa ataagaaaat gacaaattga
     1201 tacataataa ataaaatgaa ggtatg
//
LOCUS       DAENCV010000288         1201 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.293, whole genome shotgun sequence.
ACCESSION   DAENCV010000288 DAENCV010000000
VERSION     DAENCV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.293"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            7..>1201
                     /locus_tag="KQD82_003894"
     CDS             7..>1201
                     /locus_tag="KQD82_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG4382324.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSSISFSLDDTIFKTLISY
                     DIKATN"
BASE COUNT          509 a          162 c          146 g          384 t
ORIGIN      
        1 tattttatga acatacttgg attaatagtt gaatataacc catttcataa tggacacata
       61 tatcatttat ctaaatctat agaaaaaaca aatgcaacac atactgtagc aataatgagt
      121 ggaaattttc tacaaagagg cgaaccagct ttatttgata aatacactag agcaaaaatt
      181 gctgtcgaaa atggtgtaga cttagttata gaattaccaa ctttatttgc ttgtcaaagt
      241 gctgaaattt tttctcatgg tgctataaca attttaaatt cactaaactg cataaattct
      301 atatgttttg gtagtgaaga aggaaataca gaaattcttt ataatatagc tgaaatttta
      361 gcaaatgaac caaatgaatt taagttactt ttaaaaaaat atttagatga tggtctgtta
      421 tttccaacag ctagagctaa tgcacttttt gattatatac atcaagacaa aaaaaataat
      481 tttatacaaa attattcaaa ggaacaatta tcaaaaacat taaattcatc aaacaacata
      541 ctgggtatag aatacataaa aaacctcatc catttaaaca gtagtataaa accatttact
      601 atctccaggg tccaatcaga atataattcc gaagaaattg agagtgatat ttgttctgct
      661 actgctataa gaacttcact taaagaaaat acagaccttt ttcatttaaa aaatgttatt
      721 ccagaaaaaa cttataacat cctaaatgaa aaaatagata atggtttttt acctatgttt
      781 gatgacttct tctttgatat tttaaaaaca attatactta gagatatgga cactttaaaa
      841 gagtattttg aagtaaatga aggcatagaa aacagaatct ataaagatat ctttacatct
      901 gaatttctac atgaactaca attagcaatt aaatccaagc gatacacttt aaccaaaatc
      961 aaacgaactc taaacaatat attgctaggt attaaaaaaa gcgacatgca acttataaaa
     1021 aacatagata ctatacctta tataagaata cttgctttta atgataaagg tagagaaatt
     1081 ctaaaagaga taaaaaataa atctgaaata aaaataataa acaagttttc aagtatatca
     1141 ttttcattag atgatacgat ttttaaaaca ctcataagtt atgatataaa agctacaaat
     1201 a
//
LOCUS       DAENCV010000289         1176 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.294, whole genome shotgun sequence.
ACCESSION   DAENCV010000289 DAENCV010000000
VERSION     DAENCV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.294"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(236..1105)
                     /locus_tag="KQD82_003895"
     CDS             complement(236..1105)
                     /locus_tag="KQD82_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG4382325.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITSEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          447 a          182 c          108 g          439 t
ORIGIN      
        1 attctaatca atcctaaata ataatctaaa atacatcaaa aaataaacta aaaaatatcg
       61 aaaaataatt taaaagacat caaaactaaa tcttctaatc agcaattaaa agtctatttt
      121 cttagtttaa aaaataaaga tagatttttt caaaacactg gataaatagg taaaaaaata
      181 aacagttagt aaaataaatt tttactaact gtttattttt attgtttaat atgtcctata
      241 aatcctctaa gtaatatttt ccttgcaaaa attctgattc taaacctaga tttttaaaat
      301 aatcgaccat gcccatacac gcaaattttt ctgtagaata atgagtagca cctataacat
      361 taattaaatt atcttttgct atttgatgaa actctaatac tggttcaaaa tgttttaaag
      421 gcttagtaaa cccagtaata tatagattta taccaagttc agcaacttct cttgctacaa
      481 atggataact tcctccccct gctgcaatag caactaaacc atcttttaat acagaatctc
      541 cataatcata caatttcaca tcatgaccaa cagatttttt aattaattcc ttaatctctt
      601 ctattgattt tagtgatgtt ttacatataa ctcctacctc aattttatcg tcatatttac
      661 aaaatggttt gactatttct aaacccatag cttttgcaaa actaacagaa gttgaatagt
      721 cactatagtt gtctagtgga gaatgtaata cataaaaaga aattctacga tttttcatct
      781 cttccatata agatagtggt atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc
      841 ccattgcatg atgtgaaaaa attaaaatat ctttttcatc tttatctata acctgcttaa
      901 ttatttcttt atcaggaaaa gtagttgtgt atactttatt aatattttga gcattatcta
      961 atactaatcc tatgtatttt tttctaaatt ctgaagtgat aaaatcattt tcaaaattca
     1021 taaaactcca atcatcatta atatttttaa tattaaattc taaatctaat tttttataaa
     1081 gttcaattgc cttaataata accactcctt tataataata ttgaattact acatattaat
     1141 atagaaattt cacgataata aactttatac gtttaa
//
LOCUS       DAENCV010000290         1167 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.295, whole genome shotgun sequence.
ACCESSION   DAENCV010000290 DAENCV010000000
VERSION     DAENCV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.295"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(301..1047)
                     /gene="cdtR"
                     /locus_tag="KQD82_003896"
     CDS             complement(301..1047)
                     /gene="cdtR"
                     /locus_tag="KQD82_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG4382326.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          436 a          140 c          122 g          469 t
ORIGIN      
        1 tttcataaaa tatgtaggta tatatcataa aaatatgtat ttatatgata tgtcagtaca
       61 aaataacaac tgaatatttt aaagagacta tttcgtactt ttatgattta atgagctatc
      121 ttatcgaatc ataatctagg aacaattgtt ataatatcta atagtagtta tatatgatat
      181 agtcagtatg tgttttttca caatatgatt attatataga ggattcttag ggattactct
      241 ttttgtataa aaaagagatg gttcaaattt ttatcttgag acatctcctt tttctattta
      301 ttatgtttta ataatattct ttaaaatatt tctatatgta ttagatatac gacatatatg
      361 gccattactc attttaatat tatttttctt taggtcgaga gaaacaatat gatatttatt
      421 aactacaaaa gatttatgta cacggataaa gcatgcatct aaatctggta aagactttaa
      481 acttttatag aattcaaata ttctagaagt agtcactaat tttattttat gtggaatagt
      541 gcttgtttca aaatatataa cttcatcaaa tgaaattttc catagtgaga aatctgaata
      601 gatagtaaaa aaattagagc gataattttt ttctttaatg tatcttttgt gagcaatttt
      661 aatacaagct tttattttac tttcagcaat ttccatatca tgttttagta taaagtccat
      721 aacttctaac ctataatcga agatccctaa aatcatttct atatgatttg taagaaatat
      781 gattggacta atataatctg attctctaat tttttgtgct atccataatc catcacataa
      841 ttcattactt atcttatatt ttgcgtctac aactataaaa tatattctta ctttattttt
      901 ttcagtaagc tcttttaata acaaatttgc attatgatat aatcttacaa catcattatc
      961 aaaaccttct tttattaaaa tattatttat tttttctttt agtttcattc caaaacaaac
     1021 atcattatca aagattaata tatccactaa ataccctcct ataaaaaatt caaaagtaaa
     1081 aaaacaaaag tattttatag aaaatacata aaataacact aggagataaa taagattaag
     1141 gatagttaga attaaggtat atttatg
//
LOCUS       DAENCV010000291         1070 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.296, whole genome shotgun sequence.
ACCESSION   DAENCV010000291 DAENCV010000000
VERSION     DAENCV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.296"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(62..451)
                     /locus_tag="KQD82_003897"
     CDS             complement(62..451)
                     /locus_tag="KQD82_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG4382327.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          347 a          190 c           75 g          458 t
ORIGIN      
        1 aatatattac ataaatacac ttacatacac tttatattga tgctataaga aatacgtttg
       61 cttatacgtc tttctcctct tcttttagat tttttatcca ctcttcaata agtaaaatat
      121 catctttgct caaattaata ttttcatgta atgacaacaa taaacttttt atagaagaat
      181 catgaatatt tctaaaaaaa tctcttgtct caactcctat gtattccttt tctttaatta
      241 aaacttcata atgcgtatat ttatctatct tttgagaatt tagaaatctt tttactacta
      301 atctagataa aagagtcaaa gttgtcgttt gtttccattg atatttttgc tccattccta
      361 aaacaatatc tctagatgtt actttggaat ctgatttcca tataaatttc attatcttta
      421 attctgattg tggcaacttt tttatagtca tgataaaacc ttgctactca gaatatagac
      481 aagcaatatc cataataaaa tttttcgatt aaatacatct tatacggatt tatataagct
      541 cagacttata agatattttt caatcttaat cctaaattta gctatttgta cacggtaaat
      601 atatataaat aatgtcaaaa ttctcttgta ttatattgct taattttatg aaatattttg
      661 tactcagcta tctattttgg atacttgtcc agtagctctc ctttctttta aactctttga
      721 ttttaaatat ttgtatttca gtaaatccta cttcctaaat tttttattta ctacaactta
      781 ttactgaact tcctacttcc taaacttttt atttactaca acttattact gaacttctca
      841 cttactaaat ttcttactta ctataaatta taactaaact tctcacttac taaatttcct
      901 actttctaaa tttcttactt actacaaatt attactaaac ttcctactcc ctaaatttct
      961 tacttgctat aaattattac taaacttcct actccctaag tttcttactt gctacaaatt
     1021 attactaaac ttcctacttc ctaaatttct tacttgctat aaattattac
//
LOCUS       DAENCV010000292         1032 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.297, whole genome shotgun sequence.
ACCESSION   DAENCV010000292 DAENCV010000000
VERSION     DAENCV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.297"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            183..962
                     /gene="hisJ"
                     /locus_tag="KQD82_003898"
     CDS             183..962
                     /gene="hisJ"
                     /locus_tag="KQD82_003898"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG4382328.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
BASE COUNT          423 a           81 c          184 g          344 t
ORIGIN      
        1 ttaaaaaata atattaatgt taagttgaaa atagtactaa tgataagata cttaaaaggc
       61 agtttaaaat tactctaata ttagatataa taaaaattag catgattaaa aatattattt
      121 tgaagttaaa agatttatgc atttatatta gattttttct ttaaaaaagg agtgaattat
      181 ttatgaaaga tggtcatata cattcacctt attgtcctca tggaagtaag gatgattttg
      241 aaaaatatgt acaaagagca atggatgttg gaataacaga aatgacattt acagaacatt
      301 ttccgtatgc aaatggattt agagaccctg ctcctgaaaa tgatagttgt atgagtattg
      361 aagatttacc aaagtatttt aatgatgtca agaagttgaa agaaaagtat gagggtaaaa
      421 tcaagataaa tgttgggtcg gaagtggatt ttatagaagg atacgaagaa gggataaagg
      481 agaatttaaa taaatatgga aaggaattag aggattctct tttatcagta catataatta
      541 agattgatga tgattattat tgtgtagatt acagtgtaga ggagtttcaa aatttaatcg
      601 acaaattagg aagtattgaa gctgtttata ataagtatta tgaaacattg ataaaagctg
      661 taaattcaga tttaggtata cataaaccaa aaagaatagg tcatttaaat ttagttagaa
      721 agtttaatca ggtatttcca tacgattata aaggaaatac aattcttgaa gagttaataa
      781 agttaattaa agaaaaaggt tatgaacttg attataatgt ttctggaaat agaaaagagt
      841 actgcaaaga gccttatata gatggatact tattagagtt ggtaaaaaaa tacgatattc
      901 cattagtttt aggttctgat tctcactgtg cagaggaaat aaataaatac gatattattt
      961 agatatattc acacaaaaga ctgtagataa gtttgtataa aataaattta tgtacagtct
     1021 tttgtaaatt ta
//
LOCUS       DAENCV010000293         1029 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.298, whole genome shotgun sequence.
ACCESSION   DAENCV010000293 DAENCV010000000
VERSION     DAENCV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.298"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            33..917
                     /locus_tag="KQD82_003899"
     CDS             33..917
                     /locus_tag="KQD82_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG4382329.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNNKLIYSNMIDK
                     KTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVP
                     YNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNK
                     DAKFSTKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFL
                     RYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSE
                     GEIELPDPIIMENDKLMK"
BASE COUNT          472 a          109 c          124 g          324 t
ORIGIN      
        1 cgacacaata aatttaaaaa ggagaactaa atatgaaaaa aagattaata ataatgatat
       61 tgtctgttgt cttagtacta tcaagtatac ttacgatatt tgcatattca aatattaaat
      121 ataataataa taataaattg atatattcta atatgataga taaaaaaact caaaatagtg
      181 taaaggaaat cctaaaagaa aataaaatta atgaaaaaga catagataca tttattaaag
      241 cagtcaacaa ttataataaa ttgcaagtta aaatacttca aaataatata aatatatcta
      301 aatctggata ttcttcaatc aatgcaaaac aagttccata taacctagaa aaattgcaag
      361 ataattgggt gaaaaaattc cctgattata tggatgtaaa ttgtagaata actgctttta
      421 gactttttaa agattttata aattcaaata agaaatttac tggagactct attgacttga
      481 atgtcgactt ggacactata atgaataata aagatgctaa atttagcacc aaagatgtag
      541 aaaaatttat caacttcttt tctgcaatac cagctaaaga tacagatgat acaatcaaaa
      601 tttcagaaca aataaaaaat gaatggaaaa aaagaaaaat atcttttaaa aataataaaa
      661 atatatctat tataaatgga tttcttcgtt atccagaaac taaaaacgta tttataggtc
      721 atacaggcat atgcattaaa actaaaaatg gcattctatt tttagaaaaa tatggtgtta
      781 catcacctta tcaagttact aaatttaaaa ataaagaaga tgtaaaaaat tatatgttta
      841 atagattgaa gatgtcagaa ggtgaaattg aattacctga ccctataata atggaaaacg
      901 ataaacttat gaaataataa ctttaaacgc aaaatagtat ttataattac taagtcaata
      961 taatagagaa ggtctataga tttgtgtaaa tacaagctta tctacaatct tatataagaa
     1021 tgtttatat
//
LOCUS       DAENCV010000294         1025 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.299, whole genome shotgun sequence.
ACCESSION   DAENCV010000294 DAENCV010000000
VERSION     DAENCV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.299"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            779..1024
                     /locus_tag="KQD82_003900"
     CDS             779..1024
                     /locus_tag="KQD82_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG4382330.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYAEN
                     IKSNTAILDSQEEVPISRYRYKDIKEGFLKFIGDRIC"
BASE COUNT          507 a          125 c          112 g          281 t
ORIGIN      
        1 agattgacta aacaatagat atttattaat gactcaattt ataaacttta aaagttatat
       61 cccaataata ccccaaccac aaaaataaac aataaaaaat aaccaaaaaa atcagtctaa
      121 tatcataact aaatagctat atacctacat tcacaaaaac caacaaaaca atcaaataac
      181 cacttataga ctcccctcgt ctccaccatt gaaatccacg aatacctata tccaattgga
      241 tataggtatt tttatacatt tttagtttaa atattattga taaatacatt taaaaagttt
      301 taacctaaag tgaagaatct aaataaaaat aaaatattag gagaggttat cattacaata
      361 acagcttgtg atgatgaaaa agaaatacaa atcgtaataa aaaatcaagt tgataatata
      421 ttaaaaaata aaaacaaaga atatcgaatt aaaaaaatat agctcaaaag aagaaccatt
      481 acaaagttaa ttgagaggat acactactct tagatataaa aatgccagaa ctaaatagta
      541 tagatatcgc aagaaacata agaaaagtag ataataatat ttataacatc tctaataaat
      601 tatgtacagg agagatatga agtaagagat tattgatact tattaaagcc aataaatttt
      661 caagaactag aaaaacacat attaacatat atcaaaaaaa ttgaaaaaaa taaaaataag
      721 tctaacacat acaaaatcta ctctagcaaa aaaatagata gaagttcaaa aaaagaacat
      781 gctaatacat acaaaaaata aaacttttga tgctatatgt atcctagaaa aaatagaaaa
      841 agatttaaat atagaggaac ttgtaagatg tcataaaagt tatataatta acttaaatta
      901 tgcagaaaat ataaagtcaa atactgcaat attagatagt caagaagaag tacctattag
      961 tagatataga tataaagata taaaagaggg gtttctaaaa tttattggtg atagaatatg
     1021 ctaaa
//
LOCUS       DAENCV010000295         1016 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.300, whole genome shotgun sequence.
ACCESSION   DAENCV010000295 DAENCV010000000
VERSION     DAENCV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.300"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(34..642)
                     /locus_tag="KQD82_003901"
     CDS             complement(34..642)
                     /locus_tag="KQD82_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG4382331.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(902..>1016)
                     /locus_tag="KQD82_003902"
     CDS             complement(902..>1016)
                     /locus_tag="KQD82_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG4382332.1"
                     /translation="AKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRYFGK"
BASE COUNT          382 a          166 c           82 g          386 t
ORIGIN      
        1 taaatctttc tatatatttt ataataatct aaatcatgct aaatatttag acagaaaact
       61 tttttcttta atagtaaata cttttgtttg aatatcagaa taattatttg aatatctatc
      121 tataaaacca tgatttgcat ctaaaatctc aatttggata ttttgtttcc tagaaagttg
      181 gtaagaaata gaatcaacat caaatgaatc gtgctttgca aatactaata aggtaggaca
      241 ttttggaatt acatccatgt aatctctaat ccgagagcca taacaacaaa ttatgccatc
      301 acaaaaagaa tcttcactgc atctccatgc aatagttgct cccacgctat acccagcaac
      361 aaatacatat ttatactctg attttaaatc atttataagc ttttcaactt ttttataaat
      421 atcaaaacca acattattta taaaaaaatc atatgcctct tggacttttg aatatggaaa
      481 aactcttttt tctcccagaa gttcaggaca aaatacatca tatccatatt tataatactt
      541 ttgacaaaca tcttctataa agctattaat cccatagatt tcatgcaaaa caacaatagc
      601 tacatcactt ttatgtatat actttatcag attatccacc acctttacaa aacagcaaat
      661 tttttatact tagtttaata tattcttctt tacatgtcaa tttacttttc taaaatcaaa
      721 tttatttttg gattaatcat ttatttttaa aagttgtgca ttattactat taaattattc
      781 tctattatta aaaatataat cgttagaaat aaaaaataaa gtacaaataa ttaaaaaata
      841 tctcactcat aactttaaaa attataagtg agatactcct aaaatctact ttatattaaa
      901 tttattttcc aaaatatcta tttaataatc ctaaaaatgc tttaccatgt ctagcttcat
      961 ctttacacat ttcatgtact gtatcatgta tagcatctaa tcctaattct tttgct
//
LOCUS       DAENCV010000296          990 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.301, whole genome shotgun sequence.
ACCESSION   DAENCV010000296 DAENCV010000000
VERSION     DAENCV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.301"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            56..808
                     /gene="cwlD"
                     /locus_tag="KQD82_003903"
     CDS             56..808
                     /gene="cwlD"
                     /locus_tag="KQD82_003903"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG4382333.1"
                     /translation="MEYYGVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKALSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          435 a          106 c          154 g          295 t
ORIGIN      
        1 accaataaat aaagatatat caatattaaa aaaatttaaa tgtttgtact aatatatgga
       61 atattatggt gtttttataa gtatacatat acagggtgat gttgtgagaa agtacataaa
      121 acatataatt tttagttttg caatgatatg tctagtagta gtatccatat ttgaaattaa
      181 aaatatttct gaagatgtta tcaagtatat gccagtaaca aacaaaacta taattttaga
      241 tgctggtcat ggaggcattg atccaggtgc attaaataag gataagagta catctgaaaa
      301 ggatattaac ctagcaataa cacttaagct tagagagctt atagaatcaa gtggtggcct
      361 tgtaatatta actcgagaag atgacagtag cctttataaa gaagaaaata ataaaactac
      421 aagacaaaaa tataatgaga atttgaaaaa tagaaaagaa ataatatcta attctaatgc
      481 caatatgttt gtctctatac atcttaatgc atttgaacaa tcaaaatact atggagctca
      541 aactttttat cctaaagaca aacaagacag taaagcatta tcgaagtgta ttcaagaaga
      601 acttaaaaga gtagtagata agacaaacaa cagagaagtc aaaccaaggg atgatatata
      661 tcttttgaaa gaaaataata ttccatcagt actgatagaa tgtggttttt tatcaaatga
      721 aaaagagtgt aaactcttaa ctgatgaaac atatcaagaa aaaatagcat gggcaatcta
      781 cataggaata caaaaatatt taagttgaaa taactataaa aataggtcta aatatgtaaa
      841 tagcttgtat taaagcagtt aaaatgcatt aatataggct atttttattt tgacaaaaaa
      901 atatttaaaa taaaagttaa aaagttgttg acttagaata atatagatga tattatatat
      961 gagtgcccaa aaggagcacc aaaataagac
//
LOCUS       DAENCV010000297          982 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.302, whole genome shotgun sequence.
ACCESSION   DAENCV010000297 DAENCV010000000
VERSION     DAENCV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.302"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            175..681
                     /locus_tag="KQD82_003904"
     CDS             175..681
                     /locus_tag="KQD82_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG4382334.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          393 a           91 c          154 g          344 t
ORIGIN      
        1 atattttaca cttataaaat atgaaaacta cttattgcga aagtaattga tttgttgtat
       61 tataatttac atgaattatg taggacaatc aaaagtcagt attataagag tacatatatt
      121 caaattttta acaattaatt tatataatta aaaaatgaag ggtaggatga agtcatgggt
      181 tttagagaag taaaaattga ggaattacag tttaatccat ttactaagat tggcaaagaa
      241 tggttactta taacagctgg tgattcagaa aagtttaaca caatgactgc tagctggggt
      301 ggtgttggag tctattgggg taaaaatgtt gttacaactt acataagacc acagagatat
      361 acaaaagaat ttgtagatag taatgataca tttacagttg ccttctttga tgaaacgtat
      421 agagaagctt taaatatttg tggcactatt tctggtagag atataaataa aatagaaaaa
      481 gcaggactta caccatattt tgtggatgat acggtagctt ttgaagaagc taatatgatt
      541 atagtatgta aaaagctata tcatggtaat atgccaccag aaaactttga tgctaaagaa
      601 aatgataaaa aatggtatcc agagaaagat taccatacta tgtacatttc agaaattata
      661 aaagttcttg taaaagaata attataataa aaaatatatg aaaagaactt attataagta
      721 aatgactgtg ctttcttatc tgatatctaa gtagatgcta gtaactaaat aaatattaat
      781 tttaaataaa tactattata aaaaagatga actatattta ctatagttca tctttttatt
      841 tatataagca cttatataag tagagattta tttgaataaa agtatttatt tgaaatactt
      901 gagtatactt tataagtatt tttatgctaa ctagaaacac ttaatttatg gcatttgtat
      961 ttgatttatt ttaatgtaaa ta
//
LOCUS       DAENCV010000298          949 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.303, whole genome shotgun sequence.
ACCESSION   DAENCV010000298 DAENCV010000000
VERSION     DAENCV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.303"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            93..863
                     /locus_tag="KQD82_003905"
     CDS             93..863
                     /locus_tag="KQD82_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG4382335.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKVDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT          310 a          112 c          193 g          334 t
ORIGIN      
        1 gttaataaaa tttattatat atcatattgt tattatataa ttattgccat aaaaagacaa
       61 gggaaagtat aatttttaag ggggaaggat ttatgagtaa aggattcttg acacctgggg
      121 aaactgcaca agcaactata agtgcaggca ttaaaaaagc aaatatttcg acacaaaatg
      181 caattctatt aggtttattt ggaggagcat ttataggttt tggggcattg gggagtatga
      241 tagttagtca aacttttggc aaggtagacc caggggtagc tagcttttta agtgctatgg
      301 tctttccagt aggtcttatg cttgtagtag tggcaggagc agaattattc actggaaaca
      361 cattaatggc tattgcactt atggacaaaa aaataacatt aggaaaaatg ttaagaaact
      421 ggggactggt ttattttgca aatttagtag gttcaatttt gctagttgca cttgtgtttt
      481 attcttctac attgacagga gatgcagcta caaaagctat agctgtggca gaagctaagg
      541 ttaattcaac tatagcatca atgtttttaa aagcaatact ttgtaatatt ctagtcgttt
      601 tagctgtatg gatggctaca gcatctcaag atataatgtc aaaattggct tcatgttgga
      661 cagtaataat gctatttgta ttatgtggat tccaacatag tgttgcaaac atgtttttta
      721 taccaatggg aatggcatta ggagcaaata taactatggt tcaatttata actaatttgg
      781 tatttgtaac gcttggaaat atcgttggag gttccatagt agttggaggg atttattatc
      841 tttgctatgt caaaaatagt tagataaata atacgaatta aattgccttt taaagcattg
      901 atatcagtga tattgatgct ttttttaaat tatttatatt cttttatat
//
LOCUS       DAENCV010000299          917 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.304, whole genome shotgun sequence.
ACCESSION   DAENCV010000299 DAENCV010000000
VERSION     DAENCV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.304"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(189..656)
                     /locus_tag="KQD82_003906"
     CDS             complement(189..656)
                     /locus_tag="KQD82_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBG4382336.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRHFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          349 a          156 c           62 g          350 t
ORIGIN      
        1 tatatatttt ttctaaattt aaactttagt ataaatatat aagaaatatc ataatatttt
       61 taaaaactca aataaagcta aacaagtatt atattcttta ttttagaaaa ataaaaactg
      121 taagcaaatt tgttttcaca caagtttact tacagccttc acctcaattt tactttaata
      181 taatccatct attctaaaac tgattcaata tattcgacag ttacatccat tcttttagct
      241 acctcatcta ttgaaagacc actatctaaa aaatgtctac acacagatga catagtttct
      301 ccaacctcaa taatatgccc atctaaatca taaaatctaa ttgctctttg accccaccta
      361 tgctctatag ctagatgaac ataatctata tctttcattg tactcaatct ttcaacaaaa
      421 gtatcaaaat tatcttcctc aaaatataac tcagcatcat ttccattgaa cttaacttca
      481 ctgtcactct tattaataaa ttctaaccaa agcttttttt cttgtaatga tatattacct
      541 tctagtacaa cattttctcc aaaatcacag attaccttta accccaaaac atcataataa
      601 aacttttttg atttctcaat atcttcaact actactaaaa catttaaaaa cttcatatta
      661 cctccttaat atacacatac tttatttata atatataatt agaattaaac tataaaggtt
      721 tcaataaggt ctagtataaa gaaaaaaatt ataagtctta ttgaaatagt taagttaatt
      781 ttatcatata atataaattt taatcttgta aaaaacagac atgttcatac ttttaggttt
      841 aaatctatat gtattcttat taattttgct aaaactgtta tttttgtaga ctattttaga
      901 ttactgaaat atctatg
//
LOCUS       DAENCV010000300          842 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.305, whole genome shotgun sequence.
ACCESSION   DAENCV010000300 DAENCV010000000
VERSION     DAENCV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.305"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..528)
                     /locus_tag="KQD82_003907"
     CDS             complement(<1..528)
                     /locus_tag="KQD82_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG4382337.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQ"
BASE COUNT          308 a          109 c           68 g          357 t
ORIGIN      
        1 ttgtaataaa tttattattt ctataaatgt agaaatatct atatatctgt gacctaaatc
       61 aaatatatct gagataaagc taggtacttt ttctggaact cctggagtta tagctatttt
      121 tatattttta tagttattat cattcatata tggaattggt ctaatattag tcactccaat
      181 tttataaaat aaacttatag tttctagagt agtttcatca gaatcattta cgattagaac
      241 atttgtaccc tgtggtaaag atagtagatt ataaatttta tcttccctaa aagttcttct
      301 taccacaatc acttttttct tatctagtat attatttata attttgtcta atctttctac
      361 tgacattact aatatcacat catcatttat aatatcatta tcatttattt catttatgaa
      421 gtatctatta atatttatag aatctccaaa aactaatttc aaattatttt ctaaaaagtc
      481 tattaaatct tttctactat catttaccag agctactgat tttttcattt atttctcctt
      541 atataataaa tagtttccaa caaaatagtt gtataaagca atataatttt gataatttaa
      601 atttatcaaa taaaaaagaa agtgtctata atgactttat aaaagttgca ttaagacact
      661 ttctttctta taattaaaaa tcttgtcacc aaatccccct aattgttgct taaaacttaa
      721 actgtatgta tatttttcat ttctattcga ttaaattata atatacaccc aaaaaaattt
      781 attttaataa attctattga taatataatt ttgtaaatat agaattatat taaaataaat
      841 ta
//
LOCUS       DAENCV010000301          807 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.306, whole genome shotgun sequence.
ACCESSION   DAENCV010000301 DAENCV010000000
VERSION     DAENCV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.306"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>807
                     /locus_tag="KQD82_003908"
     CDS             <1..>807
                     /locus_tag="KQD82_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG4382338.1"
                     /translation="ISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGASIVAF
                     SLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLRIMGK
                     SNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQMIKSL
                     GINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKK
                     FTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGN"
BASE COUNT          240 a          120 c          131 g          316 t
ORIGIN      
        1 ttatatctgt aatgacgcaa agtttaattt tatcgattat ggcactgggg gtttatataa
       61 cttacaaaat cttagatttt ccagatatgt ctgctgatgg tagctacaca atgggtgctt
      121 ctattgtagc attttctctt actaatggta tttctcctgt agtagcaact ttaatggcta
      181 tcttatgtgg ttgtactgct ggtcttgtta cagggattct tcacattaag tttaagatat
      241 ctaatttact ttctggtatt ttagttatgg gaatgctata ctctatcaat ttaagaataa
      301 tgggaaaatc aaatattcct ctatttagct ttaaacactt attcaatggg gaaatatctc
      361 ctatagtttt agccttagct tttgtattca tctgtaaagt ccttttagat ttattcctaa
      421 aaacaggtct tggatacact ttaaaaggtg ttggtgataa ctctcaaatg attaaatccc
      481 taggtataaa tataggttct ataaaaatac taggacttat gatttcaaat ggacttatag
      541 ctttatctgg tagtcttatg gctcagttct tgggattctc agatgtaaat atgggtatag
      601 gaactttagt tcttggaatt gcctctatca taattggtat tacactattt aagaaattta
      661 cttttatcaa agatactact gcaataattg ttggctcttt tatataccaa ttcactatat
      721 actttgcaat gagtttagga atgttatcaa ctgacttaaa actaataact gccatagtta
      781 taatagcttt cctagctact ggtaact
//
LOCUS       DAENCV010000302          800 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.307, whole genome shotgun sequence.
ACCESSION   DAENCV010000302 DAENCV010000000
VERSION     DAENCV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.307"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            53..751
                     /locus_tag="KQD82_003909"
     CDS             53..751
                     /locus_tag="KQD82_003909"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG4382339.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          324 a           90 c          102 g          284 t
ORIGIN      
        1 tagattttta ttttatacat aaatatattt atatataaag gagataattt ttatgaatga
       61 aaaattaatt ggtattttgg ctggaatggg tccaaggtca acagcccctt ttgttgattt
      121 aattattgat gagtgtcaat ctcaatatgg agcaaaatat gatgatgaat ttccaaagat
      181 gatgatatat tcattaccta ccccatttta tattgaccgt ccaataaatc atgaacttat
      241 gaaagaaact ataattgatg gattacaaaa gcttgaatca actggtgtaa attttattgc
      301 aatgccatgc aattcagcac atatatactt taaagaatta aaagagtcta ttaatatacc
      361 aatattaaat attgtagaag aaacagttaa aaaattacct actatctcac aaaaagtaac
      421 tcttttttcg actagttcaa cttttgagtc tacaatttac cagaatggta taatacataa
      481 tggacatgaa tttatattta aagatgaatg gcaaataaaa ttaaacaatc ttattcaaaa
      541 tataaaaatg gataaagaaa atccacacaa tattgatatt tggaataaac ttattgaaga
      601 tgttaaaaat gaatctatag aaaatatagt aattgcttgt actgatttaa atgtagtttt
      661 agaaaaatca tatccttcaa taaatattgt tgattcatca aaatgcttgg ctgaatcagt
      721 tgtcaataaa tatctaaaat tagttaaata aatttttcat gcactatatt ataaaaaata
      781 tatgttataa gtttatttta
//
LOCUS       DAENCV010000303          753 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.308, whole genome shotgun sequence.
ACCESSION   DAENCV010000303 DAENCV010000000
VERSION     DAENCV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.308"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>753
                     /locus_tag="KQD82_003910"
     rRNA            <1..>753
                     /locus_tag="KQD82_003910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAENCV010000304          747 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.309, whole genome shotgun sequence.
ACCESSION   DAENCV010000304 DAENCV010000000
VERSION     DAENCV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.309"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            15..105
                     /locus_tag="KQD82_003911"
     tRNA            15..105
                     /locus_tag="KQD82_003911"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ser,seq:gct)
     gene            114..190
                     /locus_tag="KQD82_003912"
     tRNA            114..190
                     /locus_tag="KQD82_003912"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            276..351
                     /locus_tag="KQD82_003913"
     tRNA            276..351
                     /locus_tag="KQD82_003913"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Trp,seq:cca)
     gene            412..488
                     /locus_tag="KQD82_003914"
     tRNA            412..488
                     /locus_tag="KQD82_003914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Pro,seq:tgg)
     gene            495..571
                     /locus_tag="KQD82_003915"
     tRNA            495..571
                     /locus_tag="KQD82_003915"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Ile,seq:gat)
     gene            575..650
                     /locus_tag="KQD82_003916"
     tRNA            575..650
                     /locus_tag="KQD82_003916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:608..610,aa:Phe,seq:gaa)
     gene            658..734
                     /locus_tag="KQD82_003917"
     tRNA            658..734
                     /locus_tag="KQD82_003917"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Met,seq:cat)
BASE COUNT          176 a          171 c          215 g          185 t
ORIGIN      
        1 aaatgactga atttggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
       61 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccattcaa aaacgaggtg
      121 tagcgcagtt tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc
      181 acctcgacca acttaagctt ttagttcagt tggttagagt gcccaactca tggctagtaa
      241 ttctagggtt tgagtccatg aaggttattc ttcatagggg tgtagctcag ttggtagagc
      301 gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc cacccctgcc aaataaaaag
      361 gacttgaaaa aagttggatt ttagagtagt ataagatagt aaataagatt tcgaggtgta
      421 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
      481 ctcgaccaag aaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag
      541 gtcgctggtt cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca
      601 ggggactgaa aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc
      661 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      721 tattaccgct accatctaac tatatca
//
LOCUS       DAENCV010000305          713 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.310, whole genome shotgun sequence.
ACCESSION   DAENCV010000305 DAENCV010000000
VERSION     DAENCV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.310"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>713
                     /locus_tag="KQD82_003918"
     rRNA            <1..>713
                     /locus_tag="KQD82_003918"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          199 a          153 c          205 g          156 t
ORIGIN      
        1 ctaagcacac tgttggttat gtgtgcccaa gcattgaggc agtcaaagta ggcaaatccg
       61 ctttgataat gctgggatgt gatggggagc gaaatttagt agcgaagtag ctgatttcac
      121 actgtcaaga aaagtctcta tcgaggttaa aggtacccgt accgcaaacc gacacaggtg
      181 ggtgaggaga gtatcctaag gccagcgaga gaactgttgt taaggaactc ggcaaaatga
      241 ccccgtaact tagggataag gggtgccacc atcaggtggc cgcagagaat aggcccaagc
      301 gactgtttac caaaaacata ggtttctgct aagtcgcaag acgatgtata ggagctgacg
      361 cctgcccggt gctggaaggt taaggggatc tgttagagca atcgaagcag tgaacttaag
      421 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
      481 aagttccgac ccgcacgaaa ggcgtaacga tttgggcact gtctcaacaa cagactcggt
      541 gaaattgtaa ttccggtgaa gatgccggat acctgcgaca ggacggaaag accccatgga
      601 gctttactgt agcttgacat tgggtcttgg tactacatgt acaggatagg tgggaggctt
      661 tgaaaccagg acgccagttt tggcggagcc atccttggga taccaccctt gta
//
LOCUS       DAENCV010000306          703 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.311, whole genome shotgun sequence.
ACCESSION   DAENCV010000306 DAENCV010000000
VERSION     DAENCV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.311"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(133..273)
                     /locus_tag="KQD82_003919"
     CDS             complement(133..273)
                     /locus_tag="KQD82_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382340.1"
                     /translation="MKNENISYLALGLCFGVILGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
BASE COUNT          274 a          104 c           53 g          272 t
ORIGIN      
        1 aataattata taatatattt ttaggtcgtt taaactgtat ttttagtttt taaataacaa
       61 atattttcta ttgttgattt ccaatgattt tatacaaaaa tagtataatt gttatatgtt
      121 actatagaag ttttatttat ttttcttact gtctaatact actccaataa ctaatccaaa
      181 ggataaacca atagctaagt ttttaaataa tgaacccaaa ataacaccaa aacataatcc
      241 taacgccaaa taacttatat tttcattttt catatattac tcctttaaat ttaattgtaa
      301 cattttaact tttctaattg attcttttga agttttgact atatcaacta aacctcttat
      361 ctcatagtta ttacactcac ttagcactcc ttttactaat attttaagat atctccccta
      421 atttataaac agtagtagta aaaaatggtt tttaaacata tattttcaaa gtctaatata
      481 tataaaacta tttttatgaa tctttactaa ttccaactgc tcttaaagga ccgagtaacg
      541 acattctaac aagtatcagt accatttctt cagggtaatc tataacctaa ctaaacctaa
      601 aaaataaaat taattataaa caaaaaataa atataataaa agggcttaca aattataagt
      661 ccttttatcg aacataaaat caaattattt tccttaaata gga
//
LOCUS       DAENCV010000307          701 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.312, whole genome shotgun sequence.
ACCESSION   DAENCV010000307 DAENCV010000000
VERSION     DAENCV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.312"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            3..79
                     /locus_tag="KQD82_003920"
     tRNA            3..79
                     /locus_tag="KQD82_003920"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="KQD82_003921"
     tRNA            90..164
                     /locus_tag="KQD82_003921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="KQD82_003922"
     tRNA            174..247
                     /locus_tag="KQD82_003922"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
BASE COUNT          243 a           95 c          128 g          235 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa cttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataaatt atagtaaact tttatattgt ttaaaaagac
      301 acaggaaaca tgtatattac ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tacagtatat tctaatagag ttacagtata ttcattaata ttgtaaacat acggagggta
      421 atgtataata gtctacaaga tgtttatgaa ggcatcagtt aaatataata aatataacta
      481 attagtaaga gttatataca ttatattgta tactttatgg tatttatata ttgaaataac
      541 ttatgaagtt atttataaag agattcatga gcatattttt gtctaaacaa taagtataag
      601 aaattataaa aaattagaga ctatagtgaa tttacaattt aatgtaatat ttataagtta
      661 tactaaataa attattatac tgtgaaatgg atatataact t
//
LOCUS       DAENCV010000308          697 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.313, whole genome shotgun sequence.
ACCESSION   DAENCV010000308 DAENCV010000000
VERSION     DAENCV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.313"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            7..282
                     /locus_tag="KQD82_003923"
     CDS             7..282
                     /locus_tag="KQD82_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG4382341.1"
                     /translation="MKYIEVQKKDMLIHTINKNFDIKYSLGKIEKELNPYKFIRCHKS
                     FIVNLRYVENIKPNTAILESGEEVPISRYRYKEVKEKFLKFLGDTIC"
BASE COUNT          282 a           54 c           96 g          265 t
ORIGIN      
        1 aatgaaataa aatatataga agttcaaaaa aaagatatgc taatacacac aataaacaag
       61 aattttgata taaaatatag tttaggcaaa atagaaaaag aattaaatcc atataaattt
      121 ataagatgcc ataaaagttt tatagtaaat ttaagatatg ttgaaaatat aaagcctaat
      181 actgcaatac tggaaagtgg agaagaggta cctattagta gatatagata taaagaagtt
      241 aaggagaaat ttttaaaatt tcttggtgat acaatatgct aaactttatg actgagctat
      301 tagtagtttt atctatattt atacagtttt ctataatgaa cggagtttta atttataaaa
      361 aggatagtaa aaaaaatgaa aaaatagtat tctatattgt cttgtttctt atttatatta
      421 taattcagat aagtagatta tttaatggag gaaattactt tagtaatata aactatttat
      481 tttatatatt agcatttatt gcttggggag gatactataa ttatttttat aagatgggtt
      541 atattaagta ttccattttc ttttatctat ttagtgtata ttataaatgt ttggatgctg
      601 ttatatatag aatactattt tttgcaatta caggtaatgt tatttcatct aaagaatctt
      661 tattagtacc tcacttagaa tctataaaaa ttcagat
//
LOCUS       DAENCV010000309          696 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.314, whole genome shotgun sequence.
ACCESSION   DAENCV010000309 DAENCV010000000
VERSION     DAENCV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.314"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..665)
                     /locus_tag="KQD82_003924"
     CDS             complement(<1..665)
                     /locus_tag="KQD82_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382342.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          219 a          120 c           37 g          320 t
ORIGIN      
        1 acttttaatt tattgttatt taatctattt tgataaacat tatttatttt attattattt
       61 aaatctttaa aatctaaact tttacattgt tcatcatctt cccatattcc tgcttgttta
      121 tttccatcat ttttatataa tacaccctta ccatttttct tattatctaa aaaatatccc
      181 ttataaatgt cattattggg atatgttaat tttcccaaac cattttttaa tccgtattta
      241 aattcacctg tatatattga accatctcta taagtatatt ttcctattcc atgcatcttg
      301 tcatctttcc ataatccttc atacttttcc ttacttgaaa aaatatatat tccaaatcca
      361 tttttctttc catcaacaaa ctcaccttta tataaatcac cattatcgta tttaaatact
      421 ttttctccct ttaatttctt attgttactc ttatttttat tattattgtt atcctttata
      481 tgtatttctt ctcttctggc ttcaataaaa tctctatcat acctaaatcc gtttcttgat
      541 ttaatttttt gattaatctt cttcttatta ataaatactc tagctagttg agtaaatata
      601 aatacagatg ataagcatat aaatacacat agtattgttg taaaataact agttatattt
      661 tccataaaaa atcacccttt aaaaaattta tttaaa
//
LOCUS       DAENCV010000310          664 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.315, whole genome shotgun sequence.
ACCESSION   DAENCV010000310 DAENCV010000000
VERSION     DAENCV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.315"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..>664)
                     /locus_tag="KQD82_003925"
     rRNA            complement(<1..>664)
                     /locus_tag="KQD82_003925"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAENCV010000311          659 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.316, whole genome shotgun sequence.
ACCESSION   DAENCV010000311 DAENCV010000000
VERSION     DAENCV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.316"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            60..>659
                     /locus_tag="KQD82_003926"
     CDS             60..>659
                     /locus_tag="KQD82_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG4382343.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKLMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          231 a           55 c           86 g          287 t
ORIGIN      
        1 gtatttttta tgatatattt tataaaaaat ataatttaat catttttgag gatattatta
       61 tgttagatgc atttataaca attgtttctt ctattataga aatatggtca tgtaaaaaag
      121 tttttgatta tactagtcag tcaaagaccg acttaaaaaa actaaatatg tattttctct
      181 tttctgtttt tctcattatt tcttctttat atataaatat aggagctaat agtagaatat
      241 tgatatgtat atttgtaatg ttcgttttct ataagtctaa ctataaggtt aatatatata
      301 aatgtctggt aatctcattt ttttattggt taatattaat aggtataaat ggattaagta
      361 tgttattagt agcaacagta agcaatttgg ataatatgaa tgttttactt agtggcagtt
      421 tatatagata tgaagcaata ttcttgggaa agctaatgtt aggtatatta ctttatgttt
      481 ataaggcttc ttttaaaaat tttaaaataa gtaaaaaaga gatatacata tgtattccta
      541 tgatagcaaa tttattgtca ttttttgtac tttatgatta tattattaat cttgtaaaaa
      601 tctctcctga aagaagaact gcttttttag taatagtttt actattaata ttatctaac
//
LOCUS       DAENCV010000312          639 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.317, whole genome shotgun sequence.
ACCESSION   DAENCV010000312 DAENCV010000000
VERSION     DAENCV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.317"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            108..578
                     /locus_tag="KQD82_003927"
     CDS             108..578
                     /locus_tag="KQD82_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG4382344.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTVIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          278 a           52 c          118 g          191 t
ORIGIN      
        1 taaaaaagta aaaataaagg attaataaaa ggtactgaag agatagagaa aaaaatgtta
       61 atagaagata ctgaaaaaat agaaataaaa aattaggagg aaaatttatg aaatatcagg
      121 tttcattatt agcagtgaag gatattgaag tatcaaaaaa attttacaaa gaattatttg
      181 agcaagaaat tgaattggac ttaggtaaaa atgtaacatt taaaggagga tttgctatac
      241 aagaagactt cgcatggctt acagggatta atccagatac agttatacaa aaatcaaata
      301 atatggaact ttattttgag gttgatgatt ttgacgaatt tattgaaaaa ctcaattatt
      361 ataaagatgt agagtttgtc cataagccac taaaacatga atggcaacaa agagtagtgc
      421 gtatttatga cccagataag catattattg agataggaga gtctatggaa gttatagcta
      481 gaagatattt atctgaggga tattctgtag aagaaacttc taaaataatt caacatccaa
      541 ttgaatttgt aaagcagtgt gaaaatagta aggaatgatt gtttaaatta taattattta
      601 gtagaaatta attgtggtag aactaattca agataatat
//
LOCUS       DAENCV010000313          628 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.318, whole genome shotgun sequence.
ACCESSION   DAENCV010000313 DAENCV010000000
VERSION     DAENCV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.318"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(90..569)
                     /locus_tag="KQD82_003928"
     CDS             complement(90..569)
                     /locus_tag="KQD82_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG4382345.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          248 a          104 c           50 g          226 t
ORIGIN      
        1 agtttatgta ttatacccta atatatttta aattactagt taatcactct tttcgggtta
       61 tttatctcct atatattaaa gtgtcacacc taaatagtta aatcattatc ctcttttatc
      121 tttattgcct ttacaaatcc atctgcaatt acaaaactca tcagagaagt tataaaatat
      181 acaaatactg gaacagtaaa ataaaatcca aatcctaaat ctagaaatct cattcctttt
      241 ccagtagtac caaaagttga aataaactca attagagagt ttaaaagcaa aagatatcca
      301 ataatcctaa atctttttac attctctttt acaaataggc tatattcaga actacctaat
      361 acttctatca atgattttat aatgataaaa tatgatacac ctaaaataaa cattaatatt
      421 ccataaatag taccttgtag agttgcttct ccactaaaac agaggtacat aaaactttta
      481 aatcctcctg caacaattac taatcctaaa agaaaaaata aaatttgtaa tataccactt
      541 agtaaatttt ttataagttt attagacatc atgccaactc ctttaaaata ttttatatat
      601 agtataatac attatattat atcgtttt
//
LOCUS       DAENCV010000314          589 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.319, whole genome shotgun sequence.
ACCESSION   DAENCV010000314 DAENCV010000000
VERSION     DAENCV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.319"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            228..>589
                     /locus_tag="KQD82_003929"
     CDS             228..>589
                     /locus_tag="KQD82_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4382346.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIES"
BASE COUNT          240 a           45 c           70 g          234 t
ORIGIN      
        1 atataaaata tattgtaata ttaaaaaaat aatagtaaac tgattgcata aaaatgattt
       61 ttatataaaa tttaagcaga agaataaatt tctaacgaaa ttggatattt cttctgtaaa
      121 tacatattca aaaaaagaat tataagagtt tagtcagcag tcgtgtaagt aataaggact
      181 ttataaatat agaaatataa tgatatgagt ataagtagaa aaattttatg atttttacaa
      241 ttatttttta taaaaaaggg aggttatgtt tgttgaattt acaatcaaaa tgggtaaact
      301 ttttaaagcc aacatatgtc agtgatgaaa taaaaagaaa aaaactacaa gttataatat
      361 ttttttctat aacttatggt ttatcttatt cactaggttt attagcaata tgttttaatc
      421 atttaataga ttcagaaatt ttagctcaat ttatgatgat attgccatta tcttcagttt
      481 ctattgcaaa attttacaca gagggaagaa ataatgataa atataatttt tattcatcag
      541 taatattatt ttttattatt tactttttta tatttattat tgaatcatt
//
LOCUS       DAENCV010000315          560 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.320, whole genome shotgun sequence.
ACCESSION   DAENCV010000315 DAENCV010000000
VERSION     DAENCV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.320"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>560
                     /locus_tag="KQD82_003930"
     rRNA            <1..>560
                     /locus_tag="KQD82_003930"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          151 a          115 c          184 g          110 t
ORIGIN      
        1 tgattagcta gttggtaagg taacggctta ccaaggcgac gatcagtagc cgacctgaga
       61 gggtgatcgg ccacattgga actgagacac ggtccaaact cctacgggag gcagcagtgg
      121 ggaatattgc acaatgggcg aaagcctgat gcagcaacgc cgcgtgagtg atgaaggcct
      181 tcgggtcgta aaactctgtc ctcaaggaag ataatgacgg tacttgagga ggaagccccg
      241 gctaactacg tgccagcagc cgcggtaata cgtagggggc tagcgttatc cggatttact
      301 gggcgtaaag ggtgcgtagg cggtctttca agtcaggagt gaaaggctac ggctcaaccg
      361 tagtaagctc ttgaaactgg gagacttgag tgcaggagag gagagtggaa ttcctagtgt
      421 agcggtgaaa tgcgtagata ttaggaggaa caccagttgc gaaggcggct ctctggactg
      481 taactgacgc tgaggcacga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
      541 acgctgtaaa cgatgagtac
//
LOCUS       DAENCV010000316          550 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.321, whole genome shotgun sequence.
ACCESSION   DAENCV010000316 DAENCV010000000
VERSION     DAENCV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.321"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          205 a           56 c           60 g          229 t
ORIGIN      
        1 tttttataat cttttatgac ttatttatat ttttcatttt tataaaaaaa caataattaa
       61 gaatataact atattctaca tgtaaaaatt tttttattta aactcaattt tctgataatt
      121 tgataatttt tcaaaattta tagttgactt ttatttttta ctgtattaaa ctaattacta
      181 tacaaaacag aaaacaaaat tttcaaaatt attaaattaa aaggagaggt aaatatgaag
      241 actttattaa acaaacaagt acgatttttt agtagccaat ttagttaggg tttgtaccta
      301 agttaagaca aattgtcata acatagatac aagcccctta ggattatgat ttttaaggat
      361 tgtatctatg ttggctatag taattgttgt taaagttttt attttatcat cataatatag
      421 agccacatgg attatactgt gtggcttatt cgaatttttg ccatacagtt atatatactg
      481 tgtggttttt ttatatcaaa atcatttttt aaaaccaaat ttaaaaaacc atttaaaata
      541 ttttcaaagg
//
LOCUS       DAENCV010000317          545 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.322, whole genome shotgun sequence.
ACCESSION   DAENCV010000317 DAENCV010000000
VERSION     DAENCV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.322"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..>545)
                     /locus_tag="KQD82_003931"
     CDS             complement(<1..>545)
                     /locus_tag="KQD82_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG4382347.1"
                     /translation="CVIVYSYKITSLYIFKNFKIGILLFFYFLFSQIIFGLIISDNQF
                     DYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWH
                     LPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIF
                     NDSEIIINYHSIIQRLIFILL"
BASE COUNT          244 a           72 c           53 g          176 t
ORIGIN      
        1 ataatagtat aaatatcaat ctttgtataa tactatggta attaataatt atttcagaat
       61 cattgaaaat taatcctaag ctattaatta gaacatgcat tatagagcaa aaccatatgt
      121 tcttagtttt catataaaca taacctaaaa atatagaaag tccaatacca tatatcaaat
      181 gggctatagc tccaattatt ggagtttgag gactatatag tgtaaattgt aaaggtaagt
      241 gccataaact ccaacaagtg cctaccatta caatacctat ttttttgcca aatctatcaa
      301 ataaaatact ttgcaaaaaa gatctccaac catactcttc acataaaaaa ggataaatgt
      361 atactaatga tattatagga agaagaatat aattcaagaa attcttataa tcaaattgat
      421 tatcacttat tattaatcca aaaataattt gagaaaataa aaagtaaaaa aatagcaaaa
      481 tacctatctt aaaattttta aaaatatata aacttgttat tttataagaa taaacaataa
      541 cacat
//
LOCUS       DAENCV010000318          519 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.323, whole genome shotgun sequence.
ACCESSION   DAENCV010000318 DAENCV010000000
VERSION     DAENCV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.323"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            2..75
                     /locus_tag="KQD82_003932"
     tRNA            2..75
                     /locus_tag="KQD82_003932"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Met,seq:cat)
     gene            90..166
                     /locus_tag="KQD82_003933"
     tRNA            90..166
                     /locus_tag="KQD82_003933"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Ile,seq:cat)
     gene            190..266
                     /locus_tag="KQD82_003934"
     tRNA            190..266
                     /locus_tag="KQD82_003934"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Pro,seq:tgg)
     gene            274..350
                     /locus_tag="KQD82_003935"
     tRNA            274..350
                     /locus_tag="KQD82_003935"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:His,seq:gtg)
     gene            359..434
                     /locus_tag="KQD82_003936"
     tRNA            359..434
                     /locus_tag="KQD82_003936"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Lys,seq:ttt)
     gene            442..515
                     /locus_tag="KQD82_003937"
     tRNA            442..515
                     /locus_tag="KQD82_003937"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:474..476,aa:Cys,seq:gca)
BASE COUNT          119 a          120 c          142 g          138 t
ORIGIN      
        1 tggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
       61 ctcctattac cgctactaaa atttaatgtg ggccattagc tcagttggtt agagcgcccg
      121 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacatt aaattaatta
      181 atataaattc gaggtgtagc gcagtttggt agcgcacatg gtttgggacc atggggccgg
      241 gggttcgagt cccttcacct cgaccaatat aaggtgggta tagctcagtt ggttagagcg
      301 ccagattgtg gctctggagg tcgtgagttc gactctcatt atccacccca attcatatgg
      361 tctattagct cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg
      421 cggatagatc accaaaaagg tggcgacata gccaagtggt aaggcagtgg actgcaactc
      481 cttgatcccc agttcgaatc tgggtgtcgc ctccaaaat
//
LOCUS       DAENCV010000319          462 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.324, whole genome shotgun sequence.
ACCESSION   DAENCV010000319 DAENCV010000000
VERSION     DAENCV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.324"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..>462)
                     /locus_tag="KQD82_003938"
     CDS             complement(<1..>462)
                     /locus_tag="KQD82_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382348.1"
                     /translation="GYVLTKDSYKPTGYTPTSEYYFDNVVMESGGNTAGAYGYALITL
                     LQALPFLTVSSYNSTQATFKADHTDPTLLTKIEVFIDDVLKETITTDLTTEKTINYTL
                     EDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAG"
BASE COUNT          133 a           69 c           79 g          181 t
ORIGIN      
        1 cctgcattaa cctcaactaa ttttgttgat atatcattta aatttacatt ttcgggcagt
       61 ggcattatat tcttacttat acttaaaact ttttcagcag ttgcattatt gctgtctgta
      121 acaactattt taagcgtgtg cagtgcatta tcttctaatg tatagttaat tgttttctct
      181 gttgttaaat ctgttgttat agtttctttt agtacatcat ctataaatac ttctatctta
      241 gttaatagtg tagggtctgt gtggtcagct ttaaatgttg cttgtgtgga gttataagag
      301 gatacggtta aaaaaggtaa tgcttgtagt aatgttatta gggcataacc ataagcacca
      361 gcagtatttc caccagattc cataacaaca ttatcaaaat aatattcaga tgttggtgtg
      421 tagccagtag gcttataact atctttagtt aatacgtagc ca
//
LOCUS       DAENCV010000320          456 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.325, whole genome shotgun sequence.
ACCESSION   DAENCV010000320 DAENCV010000000
VERSION     DAENCV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.325"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          175 a           42 c           52 g          187 t
ORIGIN      
        1 actttattat agattatttt aataccatta gtactgatgg tgtattttaa aattggtata
       61 agcattacaa atacgaatat gccaactgta atatgtaata taagtagcta tatacatatg
      121 tgctgtccca tatattgtat cattgtttat attaaaaaga ttatgtaaat tggtatcaat
      181 caataaatca ttttcagaag aaatacctaa aaacttaaaa aatacctata tgtgtattta
      241 gttagatttg atatttttac atttaatttt atagtgacaa tattatatta tttttatgat
      301 gtctatttat atgcaattac tataatatca gaacttatag tatcatttgt ttcattatca
      361 ataaggtttt ttactttagt tttgtcaatt ttattaaaaa aagttattga tataaaagat
      421 aagaaggata aaactatatg acggatttca aacata
//
LOCUS       DAENCV010000321          451 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.326, whole genome shotgun sequence.
ACCESSION   DAENCV010000321 DAENCV010000000
VERSION     DAENCV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.326"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>451
                     /locus_tag="KQD82_003939"
     rRNA            <1..>451
                     /locus_tag="KQD82_003939"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          110 a          102 c          144 g           95 t
ORIGIN      
        1 actgtcaggt gggcagtttg actggggcgg tcgcctccca aaaggtaacg gaggcgctca
       61 aaggttctct cagtacggtc ggaaatcgta cgtagagtgt aaaggcaaaa gagagcttga
      121 ttgcaagaca tacaggtcga gcaaggatga aaatcggact tagtgatccg gtggttctgc
      181 gtggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      241 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcatca catcctgggg
      301 ctgtagtagg tcccaagggt tgggctgttc gcccattaaa gtggtacgcg agctgggttc
      361 agaacgtcgt gagacagttc ggtccctatc cgtcgcaggc gtaggaaatt tgagaagacc
      421 tgtccttagt acgagaggac cgggatggac g
//
LOCUS       DAENCV010000322          401 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.327, whole genome shotgun sequence.
ACCESSION   DAENCV010000322 DAENCV010000000
VERSION     DAENCV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.327"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            88..>401
                     /locus_tag="KQD82_003940"
     CDS             88..>401
                     /locus_tag="KQD82_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382349.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGG"
BASE COUNT          133 a           45 c           92 g          131 t
ORIGIN      
        1 tagaagaaaa ggtaaatggt ggtttatcta atattgcaac tagtgtaaat gagttaataa
       61 cttattgtta gagaggagag tgagaaaatg cagactgaat ggaattttgg ttataatggt
      121 tcgccacaaa gtgttatatt gaaacctggc aaatataaat ttgaatgctg gggttcttct
      181 ggaggtatca acaattcttc ttggcatact gatgctaaag gcggatattc taaaggtgaa
      241 attacattaa aaaaacaaac tacattatat gtttacgtcg gcgaaagtgg ttttgcttct
      301 tcatctacga gtaataacac taaaagtggt tttaatggcg gtggtaaagg ttatttaaat
      361 caacaggtta tgggtactta ttattctatg tacggtggtg g
//
LOCUS       DAENCV010000323          399 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.328, whole genome shotgun sequence.
ACCESSION   DAENCV010000323 DAENCV010000000
VERSION     DAENCV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.328"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            86..>399
                     /locus_tag="KQD82_003941"
     CDS             86..>399
                     /locus_tag="KQD82_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382350.1"
                     /translation="MQTEWTFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYIYVGESGYASSSTANNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGG"
BASE COUNT          134 a           43 c           90 g          132 t
ORIGIN      
        1 tagaagaaaa attgagtggg agtttatcta acattgcaac aagtgtaaat gagttgataa
       61 cttattgtta aaggagagtg aaaaaatgca gactgaatgg acttttgggt ataatggttc
      121 gccacaaagt gttatattga aacctggtaa atataaattt gaatgttggg gttcttctgg
      181 aggtatcaac aattcttctt ggtatactga tgctaaaggt ggatattcca agggtgaaat
      241 tacattaaaa aaacaaacta cattatatat ttatgtcggc gaaagtggtt atgcttcttc
      301 atctacagct aataacacta aaagtggttt taatggtggt ggcaaaggtt atttaaatca
      361 acaggttatg ggtacttatt attctatgta tggtggtgg
//
LOCUS       DAENCV010000324          393 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.329, whole genome shotgun sequence.
ACCESSION   DAENCV010000324 DAENCV010000000
VERSION     DAENCV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.329"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="KQD82_003942"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="KQD82_003942"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="KQD82_003943"
     tRNA            276..364
                     /locus_tag="KQD82_003943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
BASE COUNT          131 a           69 c           84 g          109 t
ORIGIN      
        1 taactctata tttttaaact tgtcaaaact tatggatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacacaata agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 actgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag ttg
//
LOCUS       DAENCV010000325          370 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.330, whole genome shotgun sequence.
ACCESSION   DAENCV010000325 DAENCV010000000
VERSION     DAENCV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.330"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            85..201
                     /gene="rrf"
                     /locus_tag="KQD82_003944"
     rRNA            85..201
                     /gene="rrf"
                     /locus_tag="KQD82_003944"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            208..282
                     /locus_tag="KQD82_003945"
     tRNA            208..282
                     /locus_tag="KQD82_003945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asn,seq:gtt)
     gene            287..361
                     /locus_tag="KQD82_003946"
     tRNA            287..361
                     /locus_tag="KQD82_003946"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Glu,seq:ttc)
BASE COUNT          117 a           74 c           90 g           89 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaaatgtggt tataatagcg gagaagatac acctgtttcc
      121 attccgaaca cagaagttaa gttctctagc gctgatggta cttggtggga aactacctgg
      181 gagagtagga catagccacg tgaatgttcc aaggtagctc aacggtggag caaccggctg
      241 ttaaccggta ggctgtgggt tcgagcccca cccttggagc cattatggct cgttggtcaa
      301 gaggttaaga caccgccctt tcacggcggt aacaggggtt cgattcccct acgagtcacc
      361 atttgtggga
//
LOCUS       DAENCV010000326          366 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.331, whole genome shotgun sequence.
ACCESSION   DAENCV010000326 DAENCV010000000
VERSION     DAENCV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.331"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            80..>366
                     /locus_tag="KQD82_003947"
     CDS             80..>366
                     /locus_tag="KQD82_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4382351.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KSDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKE"
BASE COUNT          169 a           39 c           48 g          110 t
ORIGIN      
        1 attgttcacc acagcattat ataatatatt aaatatgata aaatcattaa aaagtgtatc
       61 aaaaaacaat taacgtgagg tgatagcagt gaaaaaagcc tataagttta gaatgtatcc
      121 aaataaaaaa caacaagagt taattaacaa aacttttgga tgttgtagat ttgtatataa
      181 taaatatctt gctaaaagaa tagatgttta taaaagcgat aaagaaactt ttacttataa
      241 acagtgtagt tctgatttga ccaatttaaa aaaagaatta aaatggctta aggaacctga
      301 caaattttca ctacaaaacg ccttaaaaga tttagataat gcatataaaa aattctttaa
      361 agaaaa
//
LOCUS       DAENCV010000327          363 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.332, whole genome shotgun sequence.
ACCESSION   DAENCV010000327 DAENCV010000000
VERSION     DAENCV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.332"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="KQD82_003948"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="KQD82_003948"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="KQD82_003949"
     tRNA            290..361
                     /locus_tag="KQD82_003949"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAENCV010000328          323 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.333, whole genome shotgun sequence.
ACCESSION   DAENCV010000328 DAENCV010000000
VERSION     DAENCV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.333"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..>323)
                     /locus_tag="KQD82_003950"
     rRNA            complement(<1..>323)
                     /locus_tag="KQD82_003950"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           65 a           89 c           64 g          105 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gtt
//
LOCUS       DAENCV010000329          315 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.335, whole genome shotgun sequence.
ACCESSION   DAENCV010000329 DAENCV010000000
VERSION     DAENCV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.335"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..268)
                     /locus_tag="KQD82_003951"
     rRNA            complement(<1..268)
                     /locus_tag="KQD82_003951"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           82 c           53 g          103 t
ORIGIN      
        1 ggctaatccg cgttcgctcg ccgctactta cggaatcgaa tttctttctc ttccttcagg
       61 tacttagatg tttcagttcc ctgagttccc ctcattaagc tatgtattca cttaatgata
      121 cttagacatt actctaagtg agtttcctca ttcggaaatc ttcggatcaa agtttacgtg
      181 caactccccg aagcttatcg cagcttatca cgtccttcat cggctcctag tgccaaggca
      241 tccgccctgc acccttaata acttgaccag ttaaaaaggt ttgatagatt agaagctatc
      301 aacttcgata atttt
//
LOCUS       DAENCV010000330          291 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.336, whole genome shotgun sequence.
ACCESSION   DAENCV010000330 DAENCV010000000
VERSION     DAENCV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.336"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            28..102
                     /locus_tag="KQD82_003952"
     tRNA            28..102
                     /locus_tag="KQD82_003952"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60..62,aa:Thr,seq:tgt)
     gene            117..201
                     /locus_tag="KQD82_003953"
     tRNA            117..201
                     /locus_tag="KQD82_003953"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Tyr,seq:gta)
     gene            211..284
                     /locus_tag="KQD82_003954"
     tRNA            211..284
                     /locus_tag="KQD82_003954"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Gly,seq:tcc)
BASE COUNT           74 a           76 c           71 g           70 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
       61 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      121 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      181 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      241 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa g
//
LOCUS       DAENCV010000331          280 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.337, whole genome shotgun sequence.
ACCESSION   DAENCV010000331 DAENCV010000000
VERSION     DAENCV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.337"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          121 a           22 c           37 g          100 t
ORIGIN      
        1 ttaatatgaa aatataggag attagaagtg tttgataatt acagtacatg ttagatatat
       61 cattgtaaat taacatagct ttgaatataa taatgttcaa aaggcaatat ttttgaggtc
      121 tgaatccaat aataatatag tcttatgatt gtagatatca aaaaatataa gttactaaga
      181 atacgttttg tagatttata tatgattaat aactaaagct ataaataaac aaaataaaaa
      241 gagtatgttt caacctatag cttttagatt taaaatatac
//
LOCUS       DAENCV010000332          271 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.338, whole genome shotgun sequence.
ACCESSION   DAENCV010000332 DAENCV010000000
VERSION     DAENCV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.338"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            24..>271
                     /locus_tag="KQD82_003955"
     CDS             24..>271
                     /locus_tag="KQD82_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG4382352.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIV"
BASE COUNT          108 a           31 c           43 g           89 t
ORIGIN      
        1 atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag agtttcccta
       61 atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata gaagatggtg
      121 aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta aatataatat
      181 ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact ataacaaatt
      241 tagctgagta taaaagaact caaatagtaa g
//
LOCUS       DAENCV010000333          263 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.339, whole genome shotgun sequence.
ACCESSION   DAENCV010000333 DAENCV010000000
VERSION     DAENCV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.339"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          129 a           26 c           22 g           86 t
ORIGIN      
        1 aaaatgaaat aagtttgtat tttatttata taaattttaa tagaatatgt atagtatata
       61 gtagatagaa tgtataaaaa taaaaactgt tcaataagaa ttaatataca ttaattcagc
      121 tgaatccaat caaaatactt aaataaaagt aatctgaaaa taacataatt caaatcaatt
      181 tattcaaata aacctcaatt tatctcaaat cttaagaagt cattaaacaa acttgctaaa
      241 ttacaaagag aattatcaag aaa
//
LOCUS       DAENCV010000334          257 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.340, whole genome shotgun sequence.
ACCESSION   DAENCV010000334 DAENCV010000000
VERSION     DAENCV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.340"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            <1..>257
                     /gene="tuf"
                     /locus_tag="KQD82_003956"
     CDS             <1..>257
                     /gene="tuf"
                     /locus_tag="KQD82_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG4382353.1"
                     /translation="MITGAAQMDGAILVCSATDGPMPQTREHILLSRQVGVPYIVVFL
                     NKCDMVDDEELLELVEMEVRDLLTEYDFPGDDTPIVRGSAL"
BASE COUNT          101 a           34 c           57 g           65 t
ORIGIN      
        1 atgataacag gagcagcaca aatggacgga gcaatattag tttgttcagc aacagatgga
       61 ccaatgccac aaacaagaga gcatatacta ttatcaagac aagttggagt accatatata
      121 gtagtattct taaacaaatg tgacatggta gatgatgaag agttattaga gttagtagag
      181 atggaagtaa gagatttatt aacagaatat gatttcccag gagatgacac tccaatagta
      241 agaggttcag cattaat
//
LOCUS       DAENCV010000335          250 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.341, whole genome shotgun sequence.
ACCESSION   DAENCV010000335 DAENCV010000000
VERSION     DAENCV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.341"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT           88 a           27 c           21 g          114 t
ORIGIN      
        1 taaaaagaaa aaaaatacaa attattatct ttttctttat aaactttgat ttaacttatt
       61 cattaggttt actgttatat tataataaat ttattgatcc agaaaacttt gcatcattca
      121 tgatgatatt acctctatct tcagttgcta ttgcaaagtt ttatactgaa ggattaaata
      181 atgatatgta tgaattttat tcactaatca tattattttt tcttatttat tcattattaa
      241 ttgttcttaa
//
LOCUS       DAENCV010000336          250 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.342, whole genome shotgun sequence.
ACCESSION   DAENCV010000336 DAENCV010000000
VERSION     DAENCV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.342"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          113 a           12 c           29 g           96 t
ORIGIN      
        1 tgaattattt atcgtttcat ttatgtaatg aaaatgttat ataaatgaaa caataaataa
       61 tttttatttt agaaatttat atttaaataa tcaagttgaa agtgagatac taaaaaaatt
      121 aatttaggaa ttatttgatt tattcattta taaaaattaa ttactgattt gtcgaaacca
      181 ttgtaataag aaaaaaatga aaaaataaag tgtgaatatg ttgcaaatac tgtattttaa
      241 taaaaagtag
//
LOCUS       DAENCV010000337          243 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.343, whole genome shotgun sequence.
ACCESSION   DAENCV010000337 DAENCV010000000
VERSION     DAENCV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.343"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT          101 a           20 c           31 g           91 t
ORIGIN      
        1 atgcgtcaga tccaatatca acaataggaa aaatatcttc tgggctatca agtttattta
       61 atatattaaa ataaataatg aaatatgaat aaaaattagt ttaataagat gtaaattttc
      121 ttgatgatac tagtattggc tgtaggattt ttaaaataat ttttctacag ccttttaaca
      181 tataagaata gtataaatct gaaataatgt ttgaataaag gtatttaata agtatttata
      241 cta
//
LOCUS       DAENCV010000338          211 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.345, whole genome shotgun sequence.
ACCESSION   DAENCV010000338 DAENCV010000000
VERSION     DAENCV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.345"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
BASE COUNT           85 a           14 c           26 g           86 t
ORIGIN      
        1 atatacccat atatttagtt atattgcagg cagaatgtgg tttaccataa ccaaaaggat
       61 aataaaaaat atttttctat atttttatta tctttttgta aaagatttat tatttattga
      121 attaaattaa taggcagtag aggcatagtt gataaaatta atgaagaaat taaacttatg
      181 aatataaatt tattattgag ttttaatatt a
//
LOCUS       DAENCV010000339          207 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.346, whole genome shotgun sequence.
ACCESSION   DAENCV010000339 DAENCV010000000
VERSION     DAENCV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.346"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..>207)
                     /locus_tag="KQD82_003957"
     CDS             complement(<1..>207)
                     /locus_tag="KQD82_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HBG4382354.1"
                     /translation="LVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLV
                     GETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           61 a           39 c           32 g           75 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaat
//
LOCUS       DAENCV010000340          206 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.denovo.347, whole genome shotgun sequence.
ACCESSION   DAENCV010000340 DAENCV010000000
VERSION     DAENCV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.denovo.347"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(<1..167)
                     /locus_tag="KQD82_003958"
     rRNA            complement(<1..167)
                     /locus_tag="KQD82_003958"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAENCV010000341        22269 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.guided.055, whole genome shotgun sequence.
ACCESSION   DAENCV010000341 DAENCV010000000
VERSION     DAENCV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.guided.055"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            246..863
                     /locus_tag="KQD82_003959"
     CDS             246..863
                     /locus_tag="KQD82_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG4382355.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="KQD82_003960"
     CDS             971..3502
                     /locus_tag="KQD82_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG4382356.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="KQD82_003961"
     CDS             3936..4637
                     /locus_tag="KQD82_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG4382357.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /locus_tag="KQD82_003962"
     CDS             4687..5769
                     /locus_tag="KQD82_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG4382358.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="KQD82_003963"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="KQD82_003963"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG4382359.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="KQD82_003964"
     CDS             7127..7933
                     /locus_tag="KQD82_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG4382360.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="KQD82_003965"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="KQD82_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG4382361.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGVKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10403..11023
                     /locus_tag="KQD82_003966"
     CDS             10403..11023
                     /locus_tag="KQD82_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG4382362.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11189..11725)
                     /locus_tag="KQD82_003967"
     CDS             complement(11189..11725)
                     /locus_tag="KQD82_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG4382363.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11838..12512)
                     /locus_tag="KQD82_003968"
     CDS             complement(11838..12512)
                     /locus_tag="KQD82_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG4382364.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12715..14589
                     /gene="asnB"
                     /locus_tag="KQD82_003969"
     CDS             12715..14589
                     /gene="asnB"
                     /locus_tag="KQD82_003969"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG4382365.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14681..16117)
                     /locus_tag="KQD82_003970"
     CDS             complement(14681..16117)
                     /locus_tag="KQD82_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG4382366.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKGSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIVFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16446..17024
                     /locus_tag="KQD82_003971"
     CDS             16446..17024
                     /locus_tag="KQD82_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4382367.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17063..17641
                     /locus_tag="KQD82_003972"
     CDS             17063..17641
                     /locus_tag="KQD82_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4382368.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17727..18356
                     /locus_tag="KQD82_003973"
     CDS             17727..18356
                     /locus_tag="KQD82_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG4382369.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18346..21105
                     /locus_tag="KQD82_003974"
     CDS             18346..21105
                     /locus_tag="KQD82_003974"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG4382370.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGRDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21192..>22269
                     /locus_tag="KQD82_003975"
     CDS             21192..>22269
                     /locus_tag="KQD82_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382371.1"
                     /translation="MGDSKVFEKIFSNQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSK"
BASE COUNT         8567 a         2399 c         3900 g         7403 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattacat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggt catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtt gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatattaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattattt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatggt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattttttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgtgt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt atgaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt acctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtattgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acaatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggac
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc ttcctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaaa ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggc
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtgtaa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcaaa tgaattattg agcagagtag gaaggaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttaatctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agctataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
    10321 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
    10381 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ttacttgatt tgactcaaaa
    10441 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
    10501 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
    10561 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
    10621 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
    10681 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
    10741 gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
    10801 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
    10861 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
    10921 gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca
    10981 tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac
    11041 gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt ttgtattaag
    11101 gtacaaaatt tttagttctt ttgtaaacta aaatatataa aacttattta tacttagttc
    11161 aaaatattgt tatttatttt ttctacaact ataataaaat ataaaattga tatccaatca
    11221 aaaaaataac tatgaaattt aatagcttag atatgaattt ttctgttttt gagttagttt
    11281 ttataggtag taaagaaatg tttatggtaa tgggataaaa caattcaata ccttctttag
    11341 taataaggtc aagacacaag tgagatagat atcctattat aaacccactg gacaaacatg
    11401 agaaaactat attattgtta gtgaagattg tcagagattt aaaaaaacta taaataagta
    11461 ataaggcaat tagactgtga gtaaaccctc tatgtctaaa cctacttcca aataatttgt
    11521 atattaacat aaatttttta cttatgtaag aacctctcat atctatatct ggaaataaag
    11581 aacctaaata tgcaaaataa atataaatta taattaatac tattttatat gcaatatcgt
    11641 atttaactag gtatagattg tttataaagg ggagtatcaa aagtgcgaat atataacctc
    11701 ccctttcatg tgtttctttt atcatataaa aatcctcctg aatcgtcact ataaacatta
    11761 tattggtaaa tttagactat cttttattat attatatagc aaacataagt ttttttcaaa
    11821 aggattttaa tatttaatta atcttgagat tttaaatttt tgtaattttc taaagcacca
    11881 ttccaattta ctacattaaa ccagttgtca atgtattcat ttcttctatt ttgatatttt
    11941 aaatagtaag catgctccca aacatcaagt cctattatag gagttaggtt tttacttaca
    12001 gggctatcct gatttggagt agtcataata gataatttcc catctttagt agctacaagc
    12061 caagcccaac cagaaccaaa gacatctaaa gcagattttt ggaactcttg cttaaatttt
    12121 tcaaaagaac caaagtctct atcaatagct tcttttaaag attcagaagg tatggttttt
    12181 tctggtgtca ttatatcaaa aaagaattta tgattataag ctccacctgc attatttctt
    12241 acagttgtag caatatcttt aggtaaagaa tctaaatttt gcaataattc acataaagaa
    12301 taattataaa gctcaggata tttttcaaga gcagcattta atttatcaac ataagcttga
    12361 taatgcttat catgatgcag tttcattgtt tctttatcta tataaggttc aagtgcatca
    12421 tatgcataag gtaatggttt aaccttaaat ttgttatttt caggtgtaaa agcaaatgaa
    12481 gttatgcact gtgagattat aaataaagac ataataactg gtattaatat ttttttcttc
    12541 ataaaaatca cctcctaata tagtatttct agaaataaaa aaaatatgaa atattaacag
    12601 ataatttata agaatttaat agcaatttca ttttaaattg aataagtttt aaagccaaag
    12661 acaaaactat gaaatataga ataagactaa aggacagaaa ggaattttga ttatatggac
    12721 ggcaatattt caatttatta taaaggtgat atatctaaaa aagaagatat taaatttaaa
    12781 ataaaagaat ctgtaaataa tacaagactt tctagtagaa tagagtataa ggatgaggaa
    12841 aaaacttttg attttgaaag atttaatgta attgatgcta tttatggaaa taaaccattt
    12901 gaaaaagatg gaaaagttat tgtttttaat ggaaatatat ataactatca tgaaataaaa
    12961 gaagaattaa ttaaaaaagg acacaatttt actacaaatg atgatattga agtcttattg
    13021 gcatcttata tagaatttgg taagaattgt gtacataaga ttaaaggaat gttcaacttt
    13081 ataatctatg atagagaaaa tgaaagtata tttggagcaa gagatttatt tgggataaaa
    13141 ccactctatt atataaataa ggaaaatgca ataatttttt cctcagagta taagttttta
    13201 cttgaatata tgaaaaattt aaatataaat gaaagaagct tacaaagtta tttttcattt
    13261 caatatgtac ttcctgaaga tactatgata caaggaataa ggctgatacc agcaggtcac
    13321 tattttaggg tagaaaatgg tatactatct ttaaagagat ataataaact tgagtttaga
    13381 agtagtacaa aattttttta tactaaaaat cacttaggca atagagatgt gaacgaggat
    13441 gatataagaa atatagtagt agattcaata gtaactcata tggaagagga gaaagaaatt
    13501 ggaacatttt tgtctggtgg aatagattca tctattataa ctactgtggc ttcacaaatt
    13561 aatccaaata taaaatcttt ttctgcaggc tttagtgtca aaggatatag tgaattagaa
    13621 gtagcaaaga aaacagctga taagttaggt atagaaaata tccagattaa tataacacaa
    13681 gatgaatata taaaatcatt accgaatgta atttattctt tggatgaccc tatagcagac
    13741 ccttcagaag taggtcttta tttcttaatt aaagaggcag gtaaacatgt taaagtagca
    13801 ttgtctggag aaggagcaga tgaattattt ggaggatata atatatataa agagtatagt
    13861 actatgaaat cagttgtcaa ttcacctact tatattaaaa gtatattagg aagagtttca
    13921 gaacttatgc caaacataaa aggaagaaat tatctttata gagctacaac accattagaa
    13981 aaaagatata taggaaatgc aaaagttttt gaaaacagcg aagtaaaaag attctttttt
    14041 aagtataagg aaaagaatat atatgaatac ctgttgtcta atttgtatag agatgcacaa
    14101 aaaaataatt acgattatat aagtaaaatg caacatatag atgtgaatac atggcttcaa
    14161 ggcgatatac ttcaaaaaat tagcaaactt tctacagctg agcaggttga acttagagta
    14221 ccatttttat ataaagatgt atttgatgta gcaaaaaatt taagaatgga gcaaaaaata
    14281 aataagaata atacaaaagt tttacttaga gaggcgttta gagaaatagt cccagaacat
    14341 gttgttcaaa ggaaaaaatt aggatttcca actccaataa gagtttggtt aaaagattct
    14401 ctaggggata ttgtaaagga aactatttat aattcaaatg tagatgaatt tatagataaa
    14461 aaatatgcaa taaaactttt agatacgcat cttaaaggtc atagagataa ttcgagaaaa
    14521 atatggacta tatttacatt ttgtctatgg catcaattat ttattgagca taaaaatgtc
    14581 gagtattaat acttttttca atattaatca gagggcaaaa gcatgcaatg ttttcaccct
    14641 cctgattatt atggaatagg ataagtagaa ctttgattga ttagtttact atctcaataa
    14701 aattattggc aaatattttg acaagattac tttcaacagc taaatttaag ttttgtccat
    14761 catcatatcc agcggaaatc aatcctagta agtttccaaa catatctaaa agagctcctc
    14821 ctgaactacc actagagatt ggagctgtaa attgtatcat ttgaaccgtt tcaaaatcac
    14881 gaaaagctga aataattcca tctgaaactg tattaaaaag acctaaaggg cttccaattg
    14941 caactatttt ttgacctctc acaggttttt tactgacttt aacaggtatt ggcttgcatt
    15001 ttctattaac ctttatcaca gctaaatcat aatctgaatg gtattttata atttggtaag
    15061 atgtataaac tttatcatca ttttcaaatc taactaaaaa tgaataccca tcattgacta
    15121 catgaagatt cgtaagtata taaccttcat tatttattac tactccagaa ccgctcttaa
    15181 acggtttatt ttcgttattg cacacaacaa tcataacaac agaagctgat aactttgcaa
    15241 ggtcttcata agaaagttct acttcctcat cttgtacaat ttttttagat gttagatttg
    15301 tgttattaag tgtattttta gagttaggtt tattcgtagt aggagaagtc ttattggtat
    15361 tttcactgta atttatattt gtaatatgcc tttcaaaagg tatttctaat atatttggag
    15421 tcttcctaga aagttcaaat agagtagaaa attttgaaca ttcaggatat aaatcaagaa
    15481 tatctttatt tggatttata tataaaatat cacacccaag ttgtgataaa aaatataaga
    15541 aaaaaagttc ttgcctttta atatttccaa agtatactat ctttggtgaa atagtagtct
    15601 tcaccttgtt ataaaataat ttaggtagaa aatagtccat ccaacacaaa agtttaactg
    15661 caaagttttt gatgatagag gatgtacaat ttggcatact tgtggaaaat aaatctaaag
    15721 tatttttaaa tacaatcttt ttagtccaat ttaaggtgtc attttctatt ttacatggaa
    15781 agtcgatatt atataaatcc caactgttaa acttagaact atcataaata ttaaaaattt
    15841 taagcatctt atctgtttgt tctttgtcaa aatttatttt tagattgttt aatctaatat
    15901 agggaattgt aggattatta aaaaaataag tatctatatt tttaattttt tctatgtatt
    15961 tatcaattga atcatcaaca cctatatatc ttgtaaagta gacttcttta atatatgaag
    16021 aacccttatt tttataggta gtatcagata taaattcaat tagaggttca gtagactttt
    16081 ttattgaaat ctttaatggt ttaatagtac aaatcatatt ttttctccta taataagatg
    16141 atataatcta ttataaaaca acttaaaaca atatagtaca ataattttgt agtttcaaaa
    16201 taaaatatgt acttgtttat ataaatgata tataattaca tattaaacat agcttttata
    16261 catttaataa agtatgtaaa atctatgtaa aaagtaagaa atataaacat ttttttaatt
    16321 atattttaaa aatatcaagc aagttttctt cacaaatgaa taaaaaatgt tataatatga
    16381 ttaaagaaaa agataaataa ttagcaagat ttgtttagct aaaattaaca agggagcgat
    16441 aaattatggg tataacatta tcaaaaggcc aaaaggttga tttaactaaa ggaaatccag
    16501 gtcttaaaaa tattttagta ggtttaggat gggatactaa caagtatgat ggtggatttg
    16561 actttgattt agatacagca gcatttttaa caggagcaag tggaagtgtt actaatgatg
    16621 gagattttgt attttacaac aatttaaaac atacatctgg agcagttgag catctaggag
    16681 ataacagaac tggcgaaggt gatggagatg atgaacaaat agttgttgat ttatctaaga
    16741 ttcctggaga aataagcaag atttctttta ctgttacaat tcatgatgca acagaaagaa
    16801 gacaaaattt tggacaagta agtaattctt atataagaat agtagataaa gacacaaatg
    16861 aagagttaat aaagtatgaa ttaggagaag attttagtat agaaacagct atagtagtag
    16921 ctgaaatata taagcacaat ggagaatgga agtttaatgc actaggttct ggctttgaag
    16981 gtggtcttgc tgcattatgt ggaaactttg gaataaactt ataatttcaa ctataacaat
    17041 aaactaaagg gggatatttt ccatggctat agtattaaaa aaaggacaaa aaatcgattt
    17101 aactaaggga aatccaggtc ttaaaaatat aaaacttgga ctaggatggg atacaaattc
    17161 atttgatagt ggctatgatt atgatttaga tgtaagtgtt ttcatggttg gagaatctca
    17221 aagagttgaa aaagatgaag attttatatt ctacaataac ttaaaacatc catctggagc
    17281 agttgaacac ttaggtgata atagaactgg tgaaggtgat ggagatgatg aggaaatatt
    17341 agtgaattta gagacaatgc caaagcatat acaaaagata gctgtaacag ttacaatata
    17401 tgaggctgct gaaagaagac aaaattttgg acaagtaagc aactcatata taagagtatt
    17461 aaattctgaa aatgatgaag aaatattaag atatgactta ggtgaagaat ttagtataga
    17521 aactgcaatt acagtatgtg agatatataa gtataatgga gaatggaagt ttagtgcagt
    17581 tggagcagga tttgaaggtg gcttagaagc actttgtaga aactatggct taaatgttta
    17641 atttaagcaa tataatatat taaatataaa taacctatac aaataatgta taggttatat
    17701 tatataaaat tttgtggagg tttagtatgt cgattaattt atcaaaaggt gataaaatag
    17761 atttaaaaaa atctaaccca ggactgagta acatacttgt aggtctagga tgggaccctg
    17821 tgcaacaatc tggaggaggt ttttttaaat cgttatttgg tggaggacaa gctgatatag
    17881 attgtgatgc atctgtattt atgcttaatc aagaaggtaa attaagtgga ataaaagatt
    17941 taatttattt tggaaatcta aaaagtgcct gtaaatcagt tttgcataca ggagacaatc
    18001 taactggtga gggtacaggt gatgatgaac aaatcctagt taatttggat aaagttccat
    18061 caaatattca taaattattg tttgtagtaa atatatacaa ttgtgtagat agaaaacaac
    18121 actttggaat gatagaaaat gcctatataa gagttgaaga ccaaggtaat aagaaagaga
    18181 tagcaaaata caatctatct gataattatt ctgaaaaaac tactcttata gtaggagcta
    18241 tatatagaaa agatggaagt tggcaattta aagcaattgg tgaaggaaca aaagatgcgg
    18301 gtcttaaaga agtaatgcaa aatttagaca ggattgagtg tgcatatgga atttaataat
    18361 gatatcaaat attataatgt agataaagaa gaagttacaa aagacttatc tgttaatcct
    18421 gaaaaaggtt tatcagaatc agaagtcaag actagaagag aaaaatatgg tttgaatgaa
    18481 tttactccaa aagaagaggg tagtttttgg gatgatttaa aggaaagttt aagtgaacca
    18541 atgatagtta tcttgattat tgcagcagta gtaagtgcag taattgggga aactcatgat
    18601 gcaataggga ttgtaggtgc tatagcaata ggtatagcaa taggtatgat tacagaaggt
    18661 cgttctaaga aagcagcaga ggctctttct aagttgactg aaaacataga agtaaaagta
    18721 ttgagagatg gggaagtaca tcaaatttct aagagtgaat tggtaccagg tgatatagta
    18781 tatattgaaa ctggtgacat ggtacctgct gatggtagac ttatagaaag tataaacctt
    18841 aaaattagag aagatatgtt aacaggagaa tctgatgacg tatctaaaaa gtgtgatgta
    18901 gtagtttcta tggaagagat agaatctaaa ggagcagtag tagtacagga gccaatacct
    18961 gcaaaacaga taaatatggt gtttggtggt acacttgtag cttatggtag aggagctcta
    19021 gttgtaactt caattggaga taatagtgaa atgggtaaaa ttgctcaaaa cttatcagaa
    19081 acagatgagc aaactccact tcagataaaa cttggaaatt taggtggttt gatagccaaa
    19141 gtttctagtg cagtagcagg attactattt atatttatgg ttttccaaat gataagtaag
    19201 aatgtattaa atgtagatat gtctggaata ttaccgtttt tagaatcaat agacccagtt
    19261 aaaacagcat ttacagtatg tgttgcgttg atagttgcag cagtgccaga aggtcttcca
    19321 actatgataa atatgacact tgcaataaca atgcaaaaaa tggctaaaat aaatgcatta
    19381 gttaccaaaa aagaagcttg tgaaacgata ggttctgttt ctgtaatatg ttcagataaa
    19441 actggtacat taactgaaaa tagaatgact gtagaagttg catatgttga tggcagatat
    19501 attgaaagtt cagaagagga aataaatagt tattttgaag aaaactgtat gataaactct
    19561 acagctgatg tagagcacaa tgatggtgat attaagtatt taggaagtgc tactgaatgt
    19621 gcattgcttt tatattacaa aaacgtagat tatagacaag ttagaaaaaa ttctaatata
    19681 gtagctcaaa atccttttac ttcagattca aagagaatga cttctgttat aggtcgagat
    19741 aatcatcatg tgctattatc aaaaggagca ccagaagtat tacttgaatt atgttcacat
    19801 attcaaagag gaaaagacat agttccatta actgaaaata taaaacatga gattttagaa
    19861 gaaataaaga aattgcaaat aaaatcaatg agaactttag ggtttgcata taaagaaatc
    19921 tcacaagctg aagaagaagc agcagtaact gctgaaagtg atgctatgaa tgtaagtgca
    19981 atggaaaaca atttagtatt tagtggtttt gttggtataa gagacccact tagaaaagat
    20041 gttatagagt ctgtaaatac agcaaataaa gctggtgttt ctgtaaaaat gcttacagga
    20101 gataatataa atacagcaag agctataggt gaagagttag gcctattaaa aaataatatg
    20161 agagtggttg aagcttcata tatagatact ttagatgatg aagaattaaa acaagagata
    20221 caaagtattt caatcgtggc aagaagtaaa ccagatagta aaatgagaat agtttcagct
    20281 ctgcaaaaga gcggagatgt tgttgcagta acaggtgatg gtataaatga tgctccagca
    20341 ctttcaaaag cagatgttgg tatagcaatg ggtatatctg gtacagaggt atctaaaaac
    20401 gcagcagaca taattttaac tgatgatagt tttagtacca ttgtaaaagg tattaaatgg
    20461 ggtagaggaa tttatgataa cttccaaaga ttcgttcaat tccaattaac tgtaaatgta
    20521 attgcatttt tagtggctat aatatctcaa gttatgggac aagagatgcc atttactaca
    20581 atacaattat tatgggtaaa catcattatg gatggaccac cagcactagc attaggttta
    20641 gagccagtta gggaccatgt gttaaacaga aaacctgttg atagaaaagc caatataatt
    20701 tctaagtcaa tggtgtatac aatagtatta aatgcatttt atataacagc aatactaatg
    20761 ttacaatcta catttaattt cttaggagtt gatgaagcgc ataagggaac agtaatgttt
    20821 gcgttatttg cttttagtgc actgtttaat gcatttaact gtagagagtt taatgcagat
    20881 agtatcttcc ctaattttac acataacaaa ttagcactac aaataatagt gttgacagga
    20941 attgcacaag taatatttac tcagctattc caagatttct ttaactctgt agcaatggat
    21001 tttatgacat gggtaaaagt tttatgtgta gcagcaagtg taattgtggt taatgaagtt
    21061 gtaaaatgta taataagact ggtaaaacca aaaaagactg cttaaaacaa attttttaga
    21121 agaaaacaga ctgactcaaa atatgaattt tgggtcagtt ctgaataaac atttaaattg
    21181 ggaggatagt tatgggtgac tcaaaggtat ttgaaaaaat attctccaac caatcagata
    21241 gaggaaatta cactccttca aagggatatc taagttattt tatttcgtat attggtttag
    21301 aagatgaagt tttgtacaat ctcgagatat ttaaaacaaa acaaaatatt gatagcaaaa
    21361 aagatattgc tttatttact gatgttatag caaatcctag tgattttgat attataaatt
    21421 attttaaaag tggacttcaa aaatatagaa ctagcatgga agatgtagat ataaatattt
    21481 tgggatttga agagattgat tataaaataa agcaagcaat ggatagagtg ttaaaagaag
    21541 aggaaaaaga atttactaat gatagagtta aacaaaattt tatagtgaaa ataatggcat
    21601 ggataaaaat ttatatcggt gctttagata taaataaaaa tgaagctcca aaagtaatat
    21661 tttatggaga tataaaaaaa catgaagttt atttactctt aatattatac ttggcaggtt
    21721 ttgatgtttt atatcttaat ccaaattcaa agagtaatat agatatttta aaatcagaaa
    21781 gatacaatat agagtttgaa gaagccaaca taatagaaga aaaaatttct tttgaagaaa
    21841 gggttatttt aggggaaaaa atagataaaa gttctgtaaa aaaagctttt actgttgggg
    21901 cagaagcatc aaagcgtata agtgaggaat tgttaaatga tgcaggattt ataaaacctt
    21961 ggcaactcca agatagaaaa ataaaaaatc tacttctaag ttcaactgtt gatgaaatat
    22021 caatatactg gaatcaacca cttaaattaa gaccaggatt taagtttaat gatgctattg
    22081 tagaagctcc aaattttctt tctaagatta atggaatata taacgataag aatgaatata
    22141 ttaagttttt agatttatta agagattcgg aaagttcaac atttattgaa tttaatggag
    22201 atgtagatag attttcaaaa gcttttacaa gagaagcttt tagtttatcc tttttattgg
    22261 attcaaaag
//
LOCUS       DAENCV010000342        11161 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.guided.120, whole genome shotgun sequence.
ACCESSION   DAENCV010000342 DAENCV010000000
VERSION     DAENCV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.guided.120"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            278..616
                     /locus_tag="KQD82_003976"
     CDS             278..616
                     /locus_tag="KQD82_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382372.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            638..955
                     /locus_tag="KQD82_003977"
     CDS             638..955
                     /locus_tag="KQD82_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG4382373.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1105..1782
                     /locus_tag="KQD82_003978"
     CDS             1105..1782
                     /locus_tag="KQD82_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382374.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2110..2877)
                     /locus_tag="KQD82_003979"
     CDS             complement(2110..2877)
                     /locus_tag="KQD82_003979"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG4382375.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3549..4364)
                     /locus_tag="KQD82_003980"
     CDS             complement(3549..4364)
                     /locus_tag="KQD82_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG4382376.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIGNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PYIFMHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            4600..5163
                     /locus_tag="KQD82_003981"
                     /pseudo
     CDS             4600..5163
                     /locus_tag="KQD82_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5251..5871
                     /locus_tag="KQD82_003982"
     CDS             5251..5871
                     /locus_tag="KQD82_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG4382377.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            6027..6809
                     /locus_tag="KQD82_003983"
     CDS             6027..6809
                     /locus_tag="KQD82_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG4382378.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6934..7860
                     /locus_tag="KQD82_003984"
     CDS             6934..7860
                     /locus_tag="KQD82_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG4382379.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            7874..8323
                     /locus_tag="KQD82_003985"
     CDS             7874..8323
                     /locus_tag="KQD82_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG4382380.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            8683..9090
                     /locus_tag="KQD82_003986"
     CDS             8683..9090
                     /locus_tag="KQD82_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG4382381.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            9092..9322
                     /locus_tag="KQD82_003987"
     CDS             9092..9322
                     /locus_tag="KQD82_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG4382382.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9712..9915
                     /locus_tag="KQD82_003988"
     CDS             9712..9915
                     /locus_tag="KQD82_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG4382383.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9993..10346
                     /locus_tag="KQD82_003989"
     CDS             9993..10346
                     /locus_tag="KQD82_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG4382384.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10418..11050
                     /locus_tag="KQD82_003990"
     CDS             10418..11050
                     /locus_tag="KQD82_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG4382385.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4125 a         1574 c         1741 g         3721 t
ORIGIN      
        1 acatatctaa tatgtaatta caataaaaaa tgctataatt aacatattat taattcgttt
       61 tattattgta gtatggctat gaaattatga aaatatttct ctaattaaaa atatgattta
      121 tccatatttg tttattgtag atacagacta tacaaaaata aatagcactt atttcagtat
      181 aaaaacatat atagtctata tttagactaa aaaagtataa atagtctata taaaacaact
      241 taaactatga atataatcta aagtggaggg ataataattg gcaaatatag gaaaaataat
      301 aggagataga ataaaagtct atagaacaaa attgggatat agtcaagagt ttctagcaga
      361 aaaggcaggt ttacatccaa catatattgg tcagattgaa agagctgaga caaatacatc
      421 tatcaatatt ataatgaaaa ttgctactgc tttagacata ccacttgaat tgttatttgc
      481 aaatattatt acaacagaag gcgttgataa ctctgtacct ttagaatgtt atgaaatgat
      541 taataaatta actgaaaaag aacaaattgc aatgctagag ttaataaagt gcatcattaa
      601 atacaaagaa atataagtga gggaaatagt aacaataatg aattatatag gtaaaagact
      661 taaagaagaa cggaaaaaag caaatttaac ttcaaaagaa tttgcaaata tggttggagt
      721 atcgccttgg tatataactc aaatcgaaag tggtaaaaag aatccaagtc ttaaaacatt
      781 cataaaattt gtaaatatat taaatatttc tgcagatgtt cttataaaag atattacttc
      841 cactggaaaa acatatttag aaaatgatat aaatgaagag ttaaaagact taaattcaag
      901 agaactaaat ctgatatcac agattataga catcataaaa aagaataaaa tataattctt
      961 tatattttat ataaaaaaaa aggttatttg aaataaataa cctttttttg tctttttgtg
     1021 ttaacattgt cttatagtgc taatgagtac tattagacca tgttctatgg ccttatttta
     1081 atataaaacg attggagata ggctatgatt gtgattgaag gtagcgataa atttaagatt
     1141 gcaaaagaat atattgatgt agaatatact ctttttagca aagtaacttt taggtatgaa
     1201 aagttgaaat ttaaagataa tgctgaattg gaaaaaatta agatgtttaa atataaaaat
     1261 ggctacatcc ctaataaatt aaacctttct tttggatatg gattctcttc ttataaaaag
     1321 caaataatta gagaaactgt agatacttta agattgacag aaattttttc aagcgagaac
     1381 atagaagata ttaaatttat aaaagatggt acaaaaaaat tagaaattag catagagaaa
     1441 gttgtgaaat ttaaacgtcg aaaaaaaaga aattatgttt gttgctattg ccctgatatg
     1501 tatagagaca taaaactcga caaagaatct atcaataaga tatacaacag aaaaataaaa
     1561 atagaaagag aagttaatat ttttgaagat gaggatgtta taataaacaa gaaagtattg
     1621 aagtttccaa agtcttggac aaagaatatg caaaaatatt ggttaagtga aaataagtat
     1681 cccatacatt ctactgtaat tgatgatgat agatataaat gttgtaatgt acaatataca
     1741 aaaaatagag tgataatatt atattacata tataaccatt aatatacata attacaaaaa
     1801 aataatgaca aatatacata atacaaatat atcttctact ttagataaaa cagttattat
     1861 gaaaacttac tatctataaa taatagaaat gctagtatac catctataat ctaatgtcta
     1921 gtaggtttta atggcatatt ttaaatatgc catagttgat ttataagatt ttctatatca
     1981 ctttcaaaat acacactaaa tagttgcttt tcagatataa ttgcccaatt taaattttac
     2041 tatctgaaaa gttttttata tttaatttat atcttcaaaa ataccgtcta atatttctat
     2101 ataattaatt taattaatac tcttatgtaa tagattaagt actgaattta actctttaaa
     2161 tgatatttgc cctggtgctg aaacactttt tgcagctcca aaagtcaatg cagaaccaaa
     2221 tatttcacca cacaatctac ttataacacc cattcctgac attgacattg ttattattgg
     2281 tctatctgca taaattttaa acatttcatt tgtagcttca agtagcacta acacatcttt
     2341 ttcattttgc ggcataacag ctattttggg taaatctgcg cccaattcct gcattctaca
     2401 taaacgagat acaatctctt ctttttttgg agttttatta aaatcatgat tagatataat
     2461 tactttcact tcttttttat gagcaaaatt aactacttca tctatgacct catctcccat
     2521 aaataactct acatctatta aatctactaa acctgtatta gaaatttctt tattcaaagt
     2581 agtataataa tctcttgata ttaatttttc tcccccttct actacacttc taaatgtgaa
     2641 taataaaggg atatcatgta tataacttct taattcatag agaacttcct taacttcttt
     2701 aatattttct acattctcaa aaaaatcaac tcgccactct ataatatcca aacatgcatc
     2761 ttttaactct ttagcctctt ttataatatc ttttttattt ttcccaataa ttggtacaca
     2821 aattttaggt ctaccttctc caatagtaat attttttact tgaacttttc ttttcatatt
     2881 ctcctccata atcagaaatt ctataggact ttaaattcta ttttctgcac aatttaatta
     2941 tacattaaaa atactataca ttataaaaat actaatttaa aaataatttt tattctaatt
     3001 cttaaaacaa tcccacatat tttagaaaag gtactctata atatacaaac acttcttttt
     3061 tacctaaatt tattttaaca tcctataatt ttcataatat tctttaaatt ttattcatat
     3121 aaacaatatt attctggatg ctaatagaat taatctataa atcatagtat aattatgcag
     3181 aaataactcg aatttcacta cttatgaaga gattgaatat taatgaatct aaatcatttg
     3241 cattatttaa gagttttgac taaagttgaa caccatacaa aagtagctac tcaattgttt
     3301 ataccccaac caagcttaag taatgttata tagattctcg aaaaagaatt tagtatcttt
     3361 ttatttgaaa aacttatatt aaatttgatt atcttttcat aagttggtaa atgtcgatat
     3421 atcctgtatc tacaaggagt atcgacattt tctttttgga agatactttg cataagttgg
     3481 catttaaccg tataagaatg ccatcaaaag tagtaaataa gtagtaaact ctagtcatac
     3541 tactaggctt ataaccgttc catttcagct ctcgcagaac taaatgaacc atgtgcataa
     3601 tagccaagcg tcatagtaat gtttttatgc cccataatat attgcaatat attaggactc
     3661 attcctttgt tagccatatt tgtacagagt gtatgcataa agatatatgg cgtaatcttt
     3721 ggtagcttgt cctcatgggt cttgttatac ttcttgacta acgctcttaa catgctttga
     3781 taatgagttg cttgttttgg taatccatct tgtttaagaa ataggaagtt ctggtaacca
     3841 tcaacaatga ttggttctgc tttagcgttc ttacgccgtt caacgatacg ttcaaatgat
     3901 tgataagctc tttcagtcat tggaatttct cgcttaccct gtgtggtctt tggaacttct
     3961 acataatagc caatcttagt atcactcaac aattgatggt ctaccataat ggctttattt
     4021 ttcatatcca attctaaagt caaaccgcat aattcagaaa tgcgtagtcc tgtttctaag
     4081 aggataacaa catcatcata atatttacaa taagtcttat cgctctccat aaaggataat
     4141 aagcccattt cctgttctgg tgttagagcg atttttggtt ctgttgtatc ttccaaaaca
     4201 tcacttaact gaaaattaaa tggattcttt ctaatacagt cgtcttgaat tgccatgaaa
     4261 aacgacgctt tcagtgagcg cttataattg ccaatggatt tataagcaca cccatcttca
     4321 ctcattcgga tagcccagct tttagcgtct gataatttaa ccatatcaat gctttttgca
     4381 cctagtgggt cattgatagt atctatattt tgatttataa tttatacatt tttttaaaaa
     4441 cagactttta actgtaaaga atctatgtat aaacttagct atgttgtccg tgtatctaca
     4501 aaggatcttg acgagctaaa aaggcattgt gatgaagtca aagactttta cgacgactta
     4561 tcaatcaagc tcgtgcatcc ctttggggat atgttgggat tgcatggcga atttattcct
     4621 gtcagcaaac gttacatcaa tgattatatt caatatgtca cttcggattt tttggctgga
     4681 cttggttttg gagcgacaca aacccttggc gaacatgagg atatctatgt tagctacaat
     4741 ctggacactg gaaagaatgt ctatctaaaa cctgcccttg ctagtcaagg ggtaaagggt
     4801 tctattacca atgcgttagc gtctgcgttt atcggttcat tgggtggtgg taaattattc
     4861 agtaacaata tgcttgttta ctatgcggta ctattcggcg gataggcggt catagttgac
     4921 cctaaagcaa aacgcggcaa atggaaagaa acgctaccag aaatagccca tgagataaac
     4981 attgtcaatt taaccagtga tgatgaaaac aaaggattgc ttgaccatta cgtcattttg
     5041 agccgtaaaa aggattctga aagtttagcg attgatatcc taacatttct tactggtatc
     5101 tccagtcgtg acagtgataa gtttttgcag aaaaatgaca actggaaaat aataatggaa
     5161 tagtttctat gtaaatatat ttttactatc ttacctaact aattgaaaga tgatataatt
     5221 taatcaaaga tttggtaagg agggatgaat atgcatttaa ttgattttat gaatgaagat
     5281 attaaaaaga agcttgttaa aaaaacgtat aaaaagaaag agactattct ttttgctgag
     5341 caagaaaatg aatatgtaat tcttatgatt gacggtattg cagaagcatt catcttaaat
     5401 gtgaaaggaa acatctcaac gattcatctt tatagaagtg gtagtttttt tggagaaatg
     5461 gaacaattta atgatgggaa aaaacccgtt gaaattgtcg ctttcacaga ttgtaatgta
     5521 tataaacttc atagagatga ttttttaaac tggttaaggg gagattttga agctacaaaa
     5581 tttctgattc gagaaatatc tagtaaatta gttgtaaatg ctgaattaat tgaagaatta
     5641 tcatctttaa gtgttaaaga acgacttctg aggtgtatat ccttacacta ttatagaagt
     5701 gaattaaatc tgcttaccaa aaaacagctt gctagcgaag taaacgctcc aattagaagc
     5761 attaatcgtg ctataaatga atgtgtacaa gaacatctaa ttgcttatga aaacaaaaga
     5821 tttagtgtaa ttaatcagcg agaggtatta aagaatcttc ctacaacata agtataattt
     5881 caaaaaagta ttgaataata attattcaat gcttttttat ttcgggtcaa gtgacctatt
     5941 tttttttatt tcttcattat ataatactcc tatgcataat tatgttggat tgatagctaa
     6001 taaaagacag tgagaggtgt tatattatga ctaaaacaca acagaaaatg atacaaaacg
     6061 tattagaatt ttcaaaaaga gatagtagga taataggact tgcaattagc ggttcttata
     6121 tcacaaaatc gttagatgaa tattctgatt tggattttct gattgtagca gatgataaat
     6181 cctatgaaga agttatcgaa gagagattac ttattgtgga acagtttgga acattagttt
     6241 ctgcttttac tggcgaacat gttggagaac ctcgattgat aatttccctc tatgattccc
     6301 ctgttcttca tgttgatttt aagtttacaa ccttagatgg tttactagat agggttgaag
     6361 attcagctat tctctatgaa gaaaacaact gcataacaga aatttattct aaaaaagaag
     6421 catacttccc aactccagac ttacagtgga ttgaagatag attttggatt tgggttcatt
     6481 acgcttgcac taagattgga cgtagagaac tatttgaaac aattgatttt ttatcttttt
     6541 taagacaatc agtacttgcc ccattaatac aacttctctt aggtaaatta cctcgtggag
     6601 ttagaaaaat tgaattggac gcaccccaat atattgaagc attagaaaag acggttgcta
     6661 accatgaata taaaagctgt gttaatgcat tgcaggctac tatcaacctg tatcttgagt
     6721 taagagagga attatcacaa agtcctattg tccgaaaggt tgaagctgaa agggttgcat
     6781 tacagtattt tcaagattta atcaaataaa cccaaagtaa aaaaatattg aatagtaaat
     6841 attcagtgtt ttttcatttt tggtcaaatg acctagatta gttgttgtaa caattctata
     6901 attattttga aagtaacagt atggaggata actatgagtg atattttaag tagaattaca
     6961 aataaaattg gggataagaa tatagttgat aagttatcag ctctatcaaa atcagatttg
     7021 aactcacttc tattagaagt gtttgataga caagcaaata ctttaactgc aactgatatt
     7081 ctaaagtcat accaactaaa tagatttact atccctagcg gtatagatcc agaagaaatt
     7141 catgctttgg aatcaaattt attgagaaaa gcattcaata tggatatcaa gacgattatg
     7201 ctttctccct ctgccccatt aggtagctgt agcgtttttg gctccgtaga tcaatataac
     7261 gtagtaagtg ctctacgagg gacagaaaca cttgcagacc cctcaaatat gttggcaata
     7321 attattgctg ataaattaaa aagaaaagaa gaaaataata ccatacctat ccacatggct
     7381 actactgcta gggtaataag agcacaaaat tttgtaggta aaggtttata ttcacatttt
     7441 ggcttatatt gtatggtttc atctggtata gatacagggt cttacacttg tgaaaaaatg
     7501 ttgttagaaa agcacttaaa ctattataaa gatatattaa gtgaaataga aaatgttcgt
     7561 ttctctatta ttctacgcaa aagaaacgga tacaaagata atgacggatt tttcgacaga
     7621 atggttgaaa ctatcaaact tatttttcct aatatagagt tatcaattca taatgatgat
     7681 gttgataata attattataa aggtatcaat ttcaaaatat cggtcaaaca aggaaatgaa
     7741 accgttgaaa tagctgacgg tggatttgtt gactggactt atcaaatgct tggaaataaa
     7801 aaagaacgct gtttgataag tggtattggc ttagaaagat ttcttgtagc tgtttcataa
     7861 aaggagaaga aaaatggaac ttatagagtg gaattatgct tatattaccg agttagttga
     7921 gtttgcgaat gataaagaaa ttgcaaacaa tcttcgtgac aacttccccc acccttatag
     7981 ggagcaaaac gctagagatt ttattagttt ttgtttggca actccagatg ttaaacaact
     8041 tagctatgct attttttatc aagaaagggc tattggaagt atcagcctaa catttgaaga
     8101 agatacctct attaataaaa atgctgaatt aggttactgg cttgctagga actattgggg
     8161 caaagggata gcaacaaatg caataaaaga aatatgcaaa attgcctttg aaaaatacaa
     8221 ccttaacaaa attcatgcta atgtatattc ctacaataga gggtcaagta aagtattgga
     8281 aaaatgtggt tttaagattg aagaatactg tcacaattca taataaagaa tagaaacatg
     8341 attgattgcc atgtatatag tttaattcga ccgcaatagc atttattaga attaacctac
     8401 tttttaataa cagaataagt ttttcccctc tattaatttc ttatgcatct ttgtattttt
     8461 atgaaaagct agccaatgca tatttccata catcaatatg gtggtggctt acaaccaata
     8521 taaattaaat acgttttaaa gaatacacat ttttgcccta taggcagagt gtgtattttt
     8581 ttctcttttt tttgaaattt atcgaagatt taattccctc ccaccgtgat aggagtaagc
     8641 aaggttattt aactccttgc gatggaaaga gggtgaaatc ttatggttga tgaaaagaca
     8701 attgaacatc aattcgacag tttctgtaaa acagttttgc gtaactatgc tagagatatt
     8761 tatgacgaaa ataaacgcag aaatgattat ttggtatctc tcgaatcttt gtcacttgca
     8821 gaattaagta aattgagtat tttagatgat tatgattcta actacatctg tatggtttct
     8881 tacgactaca acatacgaat tgaagatgta ttaatggcac aagcaatcgg aaaacttaca
     8941 aaaagaaaac aagatataat tctactttca ttctttctca acatgacaaa tgctgacata
     9001 gcaacattga tggatttagc agaaaatacc atacattatc acaaaacaaa tgcactaaaa
     9061 gagttgaaag aactcatgga ggaacattga aatgaaacct actaaagaag tattaccttt
     9121 tacaattatt ttacttgctg tacatggcga tgtaaatgca ataaacggaa tacttaaaca
     9181 ttttgaacac tatatcattc gtctttcaca gaaaacttta tttgatgaat acggaaatcc
     9241 ctatattcat gtagaagaag aaacaaagcg aatgctagga acaaaactaa tcactgctat
     9301 tttgagattt aatttgtctt aatctcgccc tctctccatg tatcaagcta tttggtacat
     9361 ggagaatcac taattatttg tctataaaag cccacaagct attgtgattt tttataggca
     9421 aatcaatgcc gactgttctt tgacaactga ataaagtaga tagatacgtt tgtgatacag
     9481 atagccaaca aatactatac gccatgacct taatcaagag agctactata tagtgatttg
     9541 tgaataacct ttggaacggt tgttttgccg taacctgtac cacagtataa tgatacacct
     9601 gtatgacacg gttctgccca taagaatggg attggttgaa ataccaatgg agcttaccaa
     9661 agccatctat ctacttctga actacgaaca tagaaaggag tgatacattc tatgaacaat
     9721 actgatgtac ctatttggga aaaatatacc ttgacgattg aagaagcgtc aaaatacttt
     9781 cgtattggag agaaaaaatt acgtaaactt gctgatgaaa accttgattc tggctgggtc
     9841 attatgaata gcaatcgcat tcaaataaag agaaaacaat ttgagaaaat cgttgataca
     9901 cttgacgtta tctaatgaaa ttgagcctta tatgttataa tattcatatg acgtatcaag
     9961 ctcaattcgg ttattgagag gagcttctta tcatgtcaac tgaaaaaaga cgagataata
    10021 aagatagaat tttaagaact ggagagagcc agagaaaaga tggaagatac gcatataagt
    10081 atgtatattc atttggtaaa ccacaatttg tttactcttg gaaacttgta cctactgata
    10141 aacccccaaa gggaaaacgt gatgatatat cattaaggga taaggaaaaa gcaatacaaa
    10201 atgaccttga tgatagcatt gacactattg gtaagaaaat gacagtttgt cagttatacg
    10261 ctaaaaagaa tgtactaaga aagaatatcc gacttaacac taaaaaagga cgccattatc
    10321 ttatgaagat tcttaacaag gagtgattct gtgaagagat gtattgtagt agtggaatag
    10381 tcaaaaacat aatatatatt aaaggagaga tttaaatatg actattccaa gttcaaataa
    10441 ggtatatcca agaagtaatg attatcaaac tgtatatctt aaaaatgtaa taacaaggga
    10501 aaatatagaa gttggagatt acacaatata taatgacttt tataatgacc caagagattt
    10561 tgagaaaaac aatgtattgt atcattaccc tataaacaat gataaattaa taattggtaa
    10621 gttttgctca atagcatgta atgcaaaatt tctaatgact agtggaaatc actcaatgaa
    10681 atcaaaatcc acatatactt ttcccatctt ttatgaagaa tggaaactcg atatcaacca
    10741 tataacggac gcttgggaca ataagggtaa cattataatt ggaaatgatg tgtggatagg
    10801 ttatgattct ataattatgt caggtgtaaa tattggtgat ggtgcaatta ttggaacaag
    10861 agcagtagtt actaatgacg tcccaccgta ttctattgtt ggaggagtac ctgcaaaaat
    10921 tataaaaaag agatttaata atgatacaat ttcaaaatta ttagaaatta aatggtggga
    10981 ttggccatat aaaaaaattc aatcaaacat taagcatatt caatcaggaa atattgataa
    11041 attagtttaa tattttataa tttatttata ttaaacttta ctttaataat cattttataa
    11101 gagctttttc aaaatttgta tattatttta atatatataa atttaataca acattaaata
    11161 a
//
LOCUS       DAENCV010000343         8662 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.guided.154, whole genome shotgun sequence.
ACCESSION   DAENCV010000343 DAENCV010000000
VERSION     DAENCV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.guided.154"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            complement(214..2301)
                     /locus_tag="KQD82_003991"
     CDS             complement(214..2301)
                     /locus_tag="KQD82_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBG4382386.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENTDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNADILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYIVIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            2662..3240
                     /locus_tag="KQD82_003992"
     CDS             2662..3240
                     /locus_tag="KQD82_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBG4382387.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            3233..3892
                     /locus_tag="KQD82_003993"
     CDS             3233..3892
                     /locus_tag="KQD82_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBG4382388.1"
                     /translation="MNKQKFHKINLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(4103..5977)
                     /locus_tag="KQD82_003994"
     CDS             complement(4103..5977)
                     /locus_tag="KQD82_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG4382389.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMIKDRETINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(5982..7646)
                     /locus_tag="KQD82_003995"
     CDS             complement(5982..7646)
                     /locus_tag="KQD82_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG4382390.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(7660..8481)
                     /locus_tag="KQD82_003996"
     CDS             complement(7660..8481)
                     /locus_tag="KQD82_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG4382391.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
BASE COUNT         2933 a         1554 c          942 g         3233 t
ORIGIN      
        1 tggcaactct ccctcacatc ctcaattgtt gccactattt aattttttgt atgaagttaa
       61 atatattttc atcatttcaa caataaaaaa gaggtctatc tctattttga aacaacctct
      121 ttaattattt tagagtttac taagaagtct aaaaacaatt tatctaaaat attattctca
      181 actgagtaag attaatttcc aaattgagta aatttaatct tcaaacactg atttaacaat
      241 aggaataaca taatgacttc ccccacgact ctttacatct tcaatcatag caattactat
      301 atattgttta tcactatttt catctacagt aaaagcatta aaccatccaa gttctgtccc
      361 tgtaacatca tcttttgatg ctttaatttc tgctgtacca gttttcccag caatgctaat
      421 tcctttaatc atagcactat gacctgtacc atcagggctt tcaacaactt gaagtaagtc
      481 atcacgaata atttttacag cctctttaga aaacacacct ttcttccaaa attcagcctt
      541 tttaaattca agatggggtt ttatcatgtc tccatcattt acaaaagctg aatatattga
      601 tgccgtatgt actggattta caagaagctt accttgtcca tagccagtat ctgctaattg
      661 tatttcagtc tcaaatttat tgtcagtgcc aaattttgaa gaagacaatc caaattcaaa
      721 tggcatactt tcatcaaatc caagcttcag caactctttt gctaatacat cttcacctat
      781 ttttaaggct gccttagcaa aataaatatt gtcagaataa atcaaagcat ttttcatatt
      841 tgcagtattt ccataatctt ttaacgtggt taccttataa cttccccaac tagaatcttt
      901 ttgccaactt agactgctgt gtccaaagtt ttcatttgga ttaatctttc ctgttgttat
      961 accaatacca gcagtaactg gtttaaaaga agacccagga cataatcgtg ctttaaatct
     1021 attgtacatt ggtttatttt catttttatt gagttcattc catctatctt ctgacattcc
     1081 caatataaac tcatttggat tgtacgacgg actacttaca agtgataaaa cctctcctgt
     1141 tttaggattc ataactactg cacaaccttt atcatttttc atttggtcat atagtttact
     1201 ctgtagtttt gaatctatag ttagctttac atcttctcca tctacctttg gctcagttac
     1261 taaagtttct ttcttgtcac catatctatc agctattata atctcatatc catcaatacc
     1321 acgtattcta tcttccagta aattttctaa accaatctta ccaattatac tattatcgtt
     1381 gtacctctta ccctcttttt cctttagtat atctgcattt acattttgaa cataaccagt
     1441 taaatgaccg gttttttcac caaatggata tactctaacc tctgtattag atattttaac
     1501 tccaggaata ctaagaagtg actcttgtag ttccttatct tcttctttta cagtaccatc
     1561 tgtattttct tttatttttt caatagtttt tataggtacc attgaatttt cttttaccca
     1621 agatgcattt aatttctttt caatttgttc aactgataca ttcaataaag tagataattt
     1681 ttttaagtct tctatattat cactcatttt tccaggaact aatcctatca tagatgcaag
     1741 accttgacca gcaatcatta caccgttcct atccaggata cgtcctctct tacctttcag
     1801 ctttgatacc cttacagtat tatcttcttc aagttctgga aatatgacgt ttgactgcca
     1861 ttttatttta taatttttct gtctatctct agttaatagt acttcatttg aaaatgaaat
     1921 ttcacctgct aaagtatcca tcttagaatc atagtttatt actttatcat tatttttatt
     1981 ttttttaata tcattaatct taatacttat atcgtgtgca tcaattcctt catagatttt
     2041 tttatttctt aaaacaaaat cttctttttt attttcagac ttgctctttt catctaataa
     2101 atcatacatt ttctcatatt gttttttatt tgcaaaagac atatactgct taaacacttc
     2161 atctggtttg tttttattga gtccatctag tatgaatttt gctaaaaaaa caataataac
     2221 tataagcaga cacattagaa aaccaaaaat tatcattttt cttgtttttt tattttttct
     2281 catataaacc acctttatca tttaatatat tacatacgta agctttaagg ttaaaatact
     2341 cttatagaca taactaatct atgtttttat tatgttttta agtatctcat aaaaatatgt
     2401 aagcagtata attgatagta acttatatat tacacatgta agttttaaaa gtcaataata
     2461 ttatcattat ttaattattt tttacatcaa cttaaaaata aatcagttcc attatattct
     2521 tttgtattct tcaactattt tataaattaa ccttattcta ttgtaattat tttatagtat
     2581 ataactttaa cttgacaaaa cgacacaagt gtctcataat taaaagtgac acttatgtcg
     2641 ttaaaaagga ggttaaaaca tatgccaaca aaaggagaac aaactaaaca atttatcatt
     2701 gatacatcaa taatgctatt ttctgaaaaa ggatatagta atgtgacaat gaaagatata
     2761 tgtgaagcat gtaatttaag ccgtggtggc ctatatagac actttaattc accaaaagaa
     2821 atttttatca gtatattaaa cactcataaa tactactctc aaaatttgtt ggataaaggt
     2881 atcaaggaaa atatatcagc taagcatatg ctagaatatt ttttccaaca acaaaaggct
     2941 gacataaatg gcaaatattg tggatttgat tttgcaatac acgaatttgc ttttgtagaa
     3001 aaagaccaac gtgattatat gaataaaaga tacagttcag ctatcacaat gctttcaaat
     3061 ttaatcgatt atggacaatc taaaagtgaa tttaaaaaat gtgattctaa atcagttgca
     3121 atacacataa tgttcttatt tgatagcatg aaaacatctg cttccatttt gaaacttaca
     3181 aataaagaca ttgataatca aataaatatt ataaaggaca tggtgatttt atatgaataa
     3241 acaaaaattc cataaaatta atttacaaaa ctgggaacga tatacacatt tttactactt
     3301 ttcaaaaatg acaaatgtag ggtttaattt gactatagaa atagatatta ctaatttgct
     3361 taaggaaacg aaaaaaagaa atattaaatt ttatccagcg tatttatata caataactac
     3421 ttgcttaaat cgatatagag aactaaaaat cgcatatgat gaagatgaac tcggatattg
     3481 gaacacgtta acgccacttt atgcgatttt tcatgatgat aataagactt tctcatcaat
     3541 atggacagaa tatgatgaaa atttctcaat attctatgaa aattatttag ctgatgttaa
     3601 agaatatgga aaaaatcatg gttttctagc aaaaccaaat ccgccaagaa actgctatac
     3661 tgtttcctgc cttccatgga ttagctttaa gagttttact gtacataatc atggtcttga
     3721 aaattatttt ttccctacaa ttgaagcagg taaatactat caaaaagatg aaaaaatact
     3781 tcttcctctt tctataacag tgcatcacgc aactactgat ggataccatg taagttgttt
     3841 tattgattac atacaaaaaa tgtgctttaa tactaagata tggctacact aaaaatttat
     3901 acaatataaa aagaactgtc gcatgagcaa attaaatttg ctatgagaaa gctctttttt
     3961 acttacaata gttaatatga gatgaattat ttcttatact ttgaacttac caatagttaa
     4021 tatgaaatga attatttctc atactttaaa aagattgaat tactcttgat aatcttcttt
     4081 tgtagtcatt acatatcacc tactactcaa cacatattct ttgacctatt tttacatcct
     4141 tgtttatatc caaaatattt accttcttac catctggaaa caataccatt gttaaagtag
     4201 actttcctaa ttcgtgtatt ctctttaaat caacattaac aagaacgtct ccctgtttaa
     4261 ccatatctcc ttgtttaaca gcaacatcaa agccatctcc atttaatgca actgtatcaa
     4321 ttccaacatg aattaatact tctccattag ttcctttaat cccaaatgca tgtccacttg
     4381 aaaatactgt ttctaattta cctgaaatag gagaaacaat ttttccatct ataatatcaa
     4441 tagcaaaacc atcacctaac atttttttag aaaatgtttc atctggtaca ttttctaatg
     4501 caacaatttt ccccttagct ggagacacaa agttttcatt attttcatta aacactaatt
     4561 cttcgtcttc atcttcattt atagcttctt taggcactcc aaataaacat gttacaataa
     4621 agcagaatgc aaccacaaat attgatacta ataagtatgt gattaaatta tatccatcat
     4681 agatatacat taaataagaa ggtaaaactg ctaatccata tgaattagat tgaatatgta
     4741 aaattgataa tattgctgct cctaaacctg aagatgcaca cataataatc aaaggcttta
     4801 aattatatcg aagctgaatc ccaaataaaa caggctcact tataccaaaa agttgagata
     4861 gcattgctcc aactgcagtt tgtttaactt gattattttt agattttttc ataaatgcta
     4921 gacatgttcc aatattcgca aatccataca ttgcacaaag agtaataagg gcattaaatc
     4981 ctgtatttgc taatagtgat gtttcaatca ttacataagt atgatgtaag ccagtaataa
     5041 cagctaatgg atatgtaaat ccaataataa atcctccaat tcctaaaggc aaatcaatca
     5101 ataagctgat gatatcaacc atttttaatt ctattgtatg cattataggg cctacaccta
     5161 ataaaataac taacattgtt attagcataa caaagaatgg tgtgaaaata aggtctaata
     5221 cattaggcat atgtttacgg aaccattttt caagattagc tccaataaat gctgtaacaa
     5281 tagctgttaa aacacttcct tgacaaccta caataggaat aaatccaaat gccataattg
     5341 cttcaatttc atttgatggg tcagcaacag aatatgcatt aggaagtatt ggggaaacta
     5401 acataagtcc aataacaagt ccaataacag gtgttcctcc aaatacttta aatgctgacc
     5461 atacaataat tgctggtaaa aaagcaaatg ctgtttctgt aagaaccgaa acaagtgtct
     5521 gaatatacaa tggaatattt gcagaagaca ttccaaataa tccaagtaca ttatcattaa
     5581 acaaacaacc ctttaatccc aagaataatc ctgttgctgc aataactgga ataataggaa
     5641 caaatatatc tgctagagtt ctcataactt tcttaaatcc agtttcatta tttttaacta
     5701 attcatcttg ttcttttttt gataaagttt ttaatcccat tttttcaact tctgcataaa
     5761 ctttattaac tattcctgtt cctaaaataa tttgatactg tccagaagta aagaaaacac
     5821 ctttaacctc attgaccttt tccactttct tatcattaat agtttcccta tctttaatca
     5881 tcacccttag tcttgttgca caatgtgtaa tagaaataat attatcttca ccaataattt
     5941 taataagctc atttgctatt ttattatatt tattcatttt tctacctcgc tttcaataat
     6001 actgcatgaa atggtttcaa acacactgtt ttatttaata gatttatatc atctaaattt
     6061 tggaatacaa tatcatattt ttcttctact cttatttcta caggtttatc agaaaaatta
     6121 taataacaat caataactac caaatctaat tgccttctat acgcaataac atcttcactt
     6181 tctatatcaa taggttctat tgaaccataa atcaaacaat cagaatactc actatcttgt
     6241 ctcaacttta tcatcttttt ataaaattct aaaatactct ctgaattgtt aatctgactc
     6301 tgtgcattaa tatctttata attttcaata acctttaacc atggtgttgt atctgaaaaa
     6361 cctgcatttt ctttatctgc ccatggaaat ggcgttcttg cattatctcg gcttcttaaa
     6421 ttcacaaatt ctaacgcttc ttcttctgag taaccttcat ctattgaacg atagtattgg
     6481 tcaatgctag ataaatcatt aaaatcattt atggtatctc ttttaaaatt tgtcattcct
     6541 aattcctgcc cctgataaat aaatggggtt cctcttaaaa agaaatacat tgctcccaac
     6601 atttttactg catcatcatt cctttcgtat tgcaaaagat actttgttgt agctcttggt
     6661 tggtcatgat tttcaataaa attagctgcc catccatgtt cctgaacata catttgacta
     6721 tctaaaatgc attttcttaa atctggaatt gtccatgaaa ttcttttaaa ccactcacta
     6781 ccagaagcaa catctaagtc agcatgttta aagtcaaaga ccattgaaaa gaaaccatct
     6841 tctccaataa aatcacctaa ctggtcatag ctaatacctg ctgcttcagc aacagtcata
     6901 caatcatatt ttttaaacgt attttctttt aattcataaa gcattttttc tattccaggc
     6961 tgatttctaa ctgtttttgt acatttaaca agaccatcaa caccatcact ttctaaactt
     7021 tgaaaactta aattcttttt gatgaatgta atagcatcaa ctctaaatcc tgccactccc
     7081 atttgagacc aggtattaat catttggtaa agttcacttc ttaatattgg attttcccaa
     7141 ttaaggtcag gttgtttttt accaaaagaa tgaaaataat attcgtctct attctctacc
     7201 ttttcccaaa cacttccacc aaaaacagac ctccagttat ttggtaaatc gtttcctttt
     7261 ttaaaaatat aataatcatg ataaggactt tctggatttt ttaatgcttc ttgaaaccaa
     7321 ggatgttcat cacttgtatg gttaagaact aaatctaata taattttgat tcctctatct
     7381 ttactttctt taattaactc ttttaaatct tccatacttc caaattcact tgcaatatcc
     7441 aaataatcac taatatcata cccattatcg tccataggtg atttaaaaat tggacataac
     7501 cacaatgatg taatacctaa atcttttaaa taatctaaac gagaaataat tccctgtaaa
     7561 tctccaattc catcattatt actatcttga aagcttcttg gataaatttg ataaacgatt
     7621 tctttttgcc accatttctt ttccataaat tactcctttt tatatgatat ttcttctttt
     7681 taattcttta taaattccat tatcgaatat gtcctcacat atagatgtcg ctatatcttt
     7741 aagttgttga tgactatttt tcattgcaat tgtaacgtct gatgcttgaa acatgacaat
     7801 gtcattttga ccatcaccaa aacatatggt ttctttttga gttactccta atctttcttg
     7861 taatctctta attgctttgc ccttatgaaa atctttttga ataatttcat aatactgact
     7921 agaaatatct ctctgtgcta attccatttt atcttgtagt atatctttca cttcgtcaaa
     7981 taccttttca tttgaccata aacaaatttt atgtatatct tgtgacttat actcttcaat
     8041 gttattttca tatgttattt tttcttggat atgttgttta tttatacaag aattagttcc
     8101 tttcaactga ttcattgttt caaatatttc tttggctttt tgattcatga aaactttttc
     8161 ctgactctca atcgaaaaag caacttccct cttttttaat aaacaaacaa cttcctttat
     8221 caatctttga ttaaaagatt gattataaag taattcacca tgatattgaa tgtaattacc
     8281 tcctcctgca atatatccat caactcctaa tgataaaaca tcatcttgta ttgtacccat
     8341 acttcttcca gtacaaataa caacactaca atgattcttt tgacataaac gtatagcatg
     8401 ctttgcagat tctgggattc catatacctc atctctaagc gtaccatcta tatcaagtat
     8461 taataatttg tacaatttca tcttcctttc ttactatcaa aatcataata gcattcaaaa
     8521 aaaaagatat caatattttg atatctttta aaaaatatgt tcatttttag cacaaatttt
     8581 cattttcggg gaatatgttc actttctaaa tacatcttgt atttaatata aagcagttca
     8641 attaaaataa aataaggtgt tg
//
LOCUS       DAENCV010000344         2522 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.guided.259, whole genome shotgun sequence.
ACCESSION   DAENCV010000344 DAENCV010000000
VERSION     DAENCV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.guided.259"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            83..298
                     /locus_tag="KQD82_003997"
     CDS             83..298
                     /locus_tag="KQD82_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBG4382392.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            315..587
                     /locus_tag="KQD82_003998"
     CDS             315..587
                     /locus_tag="KQD82_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBG4382393.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            589..738
                     /locus_tag="KQD82_003999"
                     /pseudo
     CDS             589..738
                     /locus_tag="KQD82_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            799..1140
                     /locus_tag="KQD82_004000"
                     /pseudo
     CDS             799..1140
                     /locus_tag="KQD82_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1265..2002
                     /gene="erm(B)"
                     /locus_tag="KQD82_004001"
     CDS             1265..2002
                     /gene="erm(B)"
                     /locus_tag="KQD82_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG4382394.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2148..2432
                     /locus_tag="KQD82_004002"
     CDS             2148..2432
                     /locus_tag="KQD82_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBG4382395.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          956 a          379 c          482 g          705 t
ORIGIN      
        1 tcacaagtga ttaatcacaa atcacttgtg atttgtgatt gttgatgata aaataagagt
       61 aagaagaaat agaaagaagt gagtggttgt gggaaattta ggcgcacaaa aagcaaaacg
      121 aaatgataca ccaatcagtg caaaaaaaga tattatgggg gataagacgg ttcgtgttcg
      181 tgctgacttg caccatatta taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
      241 agttatggat aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa
      301 ggagtgaaaa tgaaatggca gttacttatg aaaaaacatt tgaaatagag atcattaacg
      361 aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaatcat gaattaaata
      421 aaaatgactc tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg
      481 taaatctttt tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa
      541 tgaatcgcta ttcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata
      601 gtcaatttta ctgacaaaca atttgaaaat cgcttaaatg ataatttaga agaattggtt
      661 caaggaaaaa aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca
      721 gggaaaacga gtttgcgaag aagatgattg agcaggcagg aacaaagtga taaattcaaa
      781 ttgtgaggga aaatatagat gaatcaagga agaattattg tgattacagg ctcaccgggg
      841 acaggaaaaa caacaactgc gtcaattgtc gcaaaggaat cagatatgga taaatcagtg
      901 catatgcaca ccgatgactt ttttcattac ttaagcaaag gcgcaatacc accacatttg
      961 ccagaatcca acaagcaaaa tctggttgtc attgaagcct ttttagaggc tgcaaagcga
     1021 tatgctcgtg gcggatatga tgtaattgta gatgggattg tcgggccatg gtttttggag
     1081 ccatggaaag ctcttgccca agaagattac gaggtacact atattgtatt aagagcgagt
     1141 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
     1201 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga
     1261 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     1321 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     1381 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     1441 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     1501 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     1561 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
     1621 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     1681 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     1741 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     1801 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     1861 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1921 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1981 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
     2041 ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc aaggagctaa
     2101 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     2161 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     2221 cgttttatgg ttgttcaaat tatcctgaat gtaccttcac tttagctgaa cattttagaa
     2281 agaaaaaact caccaaaaca aatgtaaaag aattactaga gggaaaagaa accctggtaa
     2341 aaggaatcaa aacgaaagat agaaagtcct acaatgccgt tgtaaaaatc ggagaaaagg
     2401 gatatattga ttttatctct ttttcaaaat aagcataaaa gccctttaaa gagggctttt
     2461 atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     2521 at
//
LOCUS       DAENCV010000345         1407 bp    DNA     linear   BCT 11-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-00633
            SAMEA5842931-rid12448453.guided.285, whole genome shotgun sequence.
ACCESSION   DAENCV010000345 DAENCV010000000
VERSION     DAENCV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5842931
            Sequence Read Archive: ERR3465337
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001066048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001066048.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/11/2021 01:57:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,003
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 27 of 61
            Pseudo Genes (internal stop)      :: 22 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5842931-rid12448453.guided.285"
                     /isolate="CD-15-00633"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Germany"
                     /collection_date="2013"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KQD82_004003"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KQD82_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG4382396.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          598 a          113 c          233 g          463 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaatgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatat
//
